Spreadsheet: 05f667bf-4c65-446a-8279-7afe018455db
Sheet: Sheet1

Table S3. List of tested mutants with shortened CLS. | Unnamed: 1 | Unnamed: 2
ORF | GENE | function
YBL064C | PRX1a | Mitochondrial peroxiredoxin; regulation of cell redox homeostasis
YBL078C | [NAME] | macroautophagy; membrane fusion during autophagosome formation
YBR044C | [NAME] | assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone
YBR103W | SIF2 | Sir4p-Interacting Factor
YBR217W | ATG12 | autophagy and cytoplasm-to-vacuole targeting
[NAME] | SGF29 | SAGA associated Factor; histone acetylation
YCR005C | CIT2 | Citrate synthase;  citrate, glycoxylate and  glutamate biosyntesis
YDL106C | BAS2 | PHO2, cellular reponse to phosphate starvation, purine and histidine biosyntesis
YDR080W | VPS41 | essential for membrane docking and fusion at the Golgi-to-endosome
YDR448W | ADA2 | Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT
YDR477W | SNF1 | AMP-activated serine/threonine protein kinase; signal transduction; nutirent response
YEL009C | GCN4 | transcriptional activator; amino acid biosynthesis
[NAME] | FTR1 | High affinity iron permease; transport of iron; forms complex with Fet3p
YFR008W | FAR7 | Protein involved in G1 cell cycle arrest in response to pheromone
YFR021W | ATG18 | autophagy and the cytoplasm-to-vacuole targeting
YGL252C | RTG2 | transcriptional activator of the retrograde (RTG) and TOR signaling pathways
YGR252W | GCN5 | histone acetyltransferase; catalytic subunit of SAGA
[NAME] | ECM34 | cell wall organisation and biogenesis
YHR013C | ARD1 | Subunit of the N-terminal acetyltransferase [NAME] (Nat1p, Ard1p, Nat5p)
YHR171W | ATG7 | autophagy
YIL033C | BCY1 | regulatory subunit of the cyclic AMP-dependent protein kinase (PKA)
YIL065C | [NAME] | mitochondrial fission
YIL098C | [NAME] | mitochondrial matrix protein
[NAME] | POG1 | putative transcriptional activator that promotes recovery from pheromone induced arrest
YIR024C | GIF1 | unknown function; in mitochondrion, proposed respiration
YJR004C | SAG1 | Alpha-agglutinin of alpha-cells, agglutination during conjugation with cellular fusion
YJR127C | ZMS1 | RSF2, required for glycerol-based growth, respiration
YKL040C | NFU1 | protein involved in iron metabolism in mitochondria
YKL041W | VPS24 | late endosome to vacuole transport
YLR079W | SIC1 | Inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition
YLR182W | [NAME] | Transcription cofactor that regulates transcription at the G1/S transition
[NAME] | SIR3 | gene silencing at HM and telomeres.
YMR058W | FET3 | Ferro-O2-oxidoreductase;high-affinity iron uptake and resistance to copper ion toxicity
YMR135C | DCR1 | [NAME]; Protein of unknown function; negative regulation of gluconeogenesis
YMR139W | [NAME] | glycogen synthase kinase 3 activity
YMR159C | ATG16 | essential for macroautophagy
YMR169C | [NAME] | Cytoplasmic aldehyde dehydrogenase
YNL082W | PMS1 | ATP-binding protein required for mismatch repair in mitosis and meiosis
YNL167C | SKO1 | Suppressor of PKA Overexpression; negative regulation of transcription
YNL242W | ATG2 | Peripheral membrane protein required for vesicle formation during autophagy
YOL013C | HRD1 | Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation
YOL067C | RTG1 | Transcription factor involved in interorganelle communication
YOL076W | MDM20 | mitochondrion inheritance, cytoskeleton biogenesis
YOR179C | [NAME] | Subunit of the APT subcomplex of cleavage and polyadenylation factor
YPL031C | PHO85 | Cyclin-dependent kinase, involved in respons to nutirent levels
YPL057C | SUR1 | mannosyltransferase activity; sphingolipid metabolism
YPL120W | VPS30 | ATG6, autophagy and cytoplasmic-to-vacuole targeting
YPL149W | ATG5 | autophagy and cytoplasm-to-vacuole targeting
YBL106C | SRO77b | Protein with roles in exocytosis and cation homeostasis
YBR128C | ATG14 | Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I
[NAME] | YIH1 | protein impacting gene expression in response to starvation
YDL149W | ATG9 | [NAME] protein involved in formation of Cvt and autophagic vesicles
YDR226W | ADK1 | Adenylate kinase
YER020W | GPA2 | Subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p
YGL096W | [NAME] | transcription factor
YGL153W | PEX14 | Peroxisomal membrane peroxin
YGR141W | VPS62 | vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins
YHR096C | [NAME] | Hexose transporter with moderate affinity for glucose
YJL187C | SWE1 | Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity
YJL210W | PEX2 | RING-finger peroxin, peroxisomal membrane protein
YKR035W-A | DID2 | Class E protein of the vacuolar protein-sorting ([NAME]) pathway
[NAME] | ATG10 | E2-like conjugating enzyme; cvt and autophagy pathways
YLR043C | TRX1 | Cytoplasmic thioredoxin isoenzyme; protects cells against oxidative and reductive stress
YLR055C | SPT8 | required for SAGA-mediated inhibition at some promoters
YLR207W | HRD3 | plays a central role in ER-associated protein degradation
YLR236C | YLR236C | Dubious open reading frame
[NAME] | ADE17 | Purine biosyntesis pathway
YNL257C | [NAME] | Protein that activates transcription through interaction with DNA-bound Snf1p
YPR185W | [NAME] | autophagy
aMutants printed in bold had strong CLS shortening (more that 3 days difference compared to WT).
bMutants not printed in bold had weaker effects (3 days or less difference compared to WT)