Spreadsheet: 38e1f61e-0d34-4035-aed1-48fd875b15b1
Sheet: Sheet1

PA14_Locus | PAO1 Locus | mutant | Plate | Well | Mutant ID | Sequence | GID | gene Name |  | PA14_Locus | PAO1 Locus | mutant | LT50 mutant/WT | STEM | Functional Class | Functional Class Short Name
PA14_30650 | PA2586 | gacA | PAMr_nr_mas_06_3 | G12 | 34781 | correct | GID3719 | gacA | response regulator GacA | PA14_30650 | PA2586 | gacA | 2.11743475846752 | 0 | 25 | Transcriptional regulators
PA14_08370 | PA0652 | vfr | PAMr_nr_mas_12_4 | A4 | 52692 | correct | GID3659 | vfr | cyclic AMP receptor-like protein | PA14_08370 | PA0652 | vfr | 1.8046875 | 0 | 25 | Transcriptional regulators
PA14_09300 | PA4223 | pchH | PAMr_nr_mas_01_2 | E5 | 23790 | correct | GID610 | pchH | putative ATP-binding component of ABC transporter | PA14_09300 | PA4223 | pchH | 1.78 | 0.1 | 27 | Transport of small molecules
PA14_12030 | PA4005 | PA4005 | PAMr_nr_mas_01_1 | F9 | 23465 | correct | GID4961 |  | conserved hypothetical protein | PA14_12030 | PA4005 | PA4005 | 1.75741155100853 | 0.331335918155831 | 14 | Hypothetical, unclassified, unknown
PA14_27700 |  | PA14_27700 | PAMr_nr_mas_05_3 | H5 | 32578 | correct | GID6170 |  | putative transcriptional regulator | PA14_27700 |  | PA14_27700 | 1.75559202127732 | 0.102705231814679 | 25 | Transcriptional regulators
PA14_69000 | PA5224 | pepP | PAMr_nr_mas_08_1 | E7 | 37818 | correct | GID1040 | pepP | aminopeptidase P | PA14_69000 | PA5224 | pepP | 1.75286464885224 | 0.146012399418579 | 26 | Translation, post-translational modification, degradation
PA14_61680 | PA4664 | PA4664 | PAMr_nr_mas_15_3 | C8 | 46240 | correct | GID2803 |  | putative methyl transferase | PA14_61680 | PA4664 | PA4664 | 1.73 | 0.09 | 4 | Biosynthesis of cofactors, prosthetic groups and carriers
PA14_52580 | PA0904 | lysC | PAMr_nr_mas_09_2 | E12 | 40436 | correct | GID1504 | lysC | aspartate kinase alpha and beta chain | PA14_52580 | PA0904 | lysC | 1.72348705125364 | 0.0746541880600337 | 2 | Amino acid biosynthesis and metabolism
PA14_30580 | PA2591 | vqsR | PAMr_nr_mas_12_4 | C2 | 52787 | correct | [NAME] | vqsR | transcriptional regulator, LuxR family | PA14_30580 | PA2591 | vqsR | 1.7 | 0.09 | 25 | Transcriptional regulators
PA14_72390 | PA5484 | kinB | PAMr_nr_mas_07_1 | D4 | 35639 | correct | GID568 | kinB | putative two-component sensor | PA14_72390 | PA5484 | kinB | 1.68 | 0.12 | 28 | Two-component regulatory systems
PA14_04410 | PA0337 | ptsP | PAMr_nr_mas_07_2 | G7 | 36275 | correct | GID245 | ptsP | phosphoenolpyruvate-protein phosphotransferase | PA14_04410 | PA0337 | ptsP | 1.65199797673242 | 0 | 27 | Transport of small molecules
PA14_54640 | PA0745 | PA0745 | PAMr_nr_mas_08_1 | B1 | 37629 | correct | GID2844 |  | probable enoyl-CoA hydratase/isomerase | PA14_54640 | PA0745 | PA0745 | 1.6128287511955 | 0.0583736030916402 | 20 | Putative enzymes
PA14_19120 | PA3477 | rhlR | PAMr_nr_mas_08_1 | G7 | 37943 | correct | [NAME] | rhlR | acylhomoserine lactone dependent transcriptional regulator | PA14_19120 | PA3477 | rhlR | 1.61 | 0.1 | 25 | Transcriptional regulators
PA14_31580 | PA2550 | PA2550 | PAMr_nr_mas_06_3 | H7 | 34827 | correct | GID1348 |  | putative acyl-CoA dehydrogenase | PA14_31580 | PA2550 | PA2550 | 1.60927882346946 | 0.057572925558859 | 13 | Fatty acid and phospholipid metabolism
PA14_22020 | PA3244 | minD | PAMr_nr_mas_12_4 | D7 | 52889 | correct | GID3011 | minD | cell division inhibitor MinD | PA14_22020 | PA3244 | minD | 1.6 | 0.35 | 6 | Cell division
PA14_43900 | PA1592 | PA1592 | ExMr_nr_mas_01_2 | F7 | 6472 | correct | GID5340 |  | conserved hypothetical protein | PA14_43900 | PA1592 | PA1592 | 1.54 | 0.22 | 14 | Hypothetical, unclassified, unknown
PA14_69810 | PA5288 | glnK | PAMr_nr_mas_06_2 | A4 | 33817 | correct | GID5042 | glnK | Nitrogen regulatory protein PII | PA14_69810 | PA5288 | glnK | 1.53 | 0.41 | 8 | Central intermediary metabolism
PA14_52670 | PA0898 | aruD | PAMr_nr_mas_05_1 | F1 | 31424 | correct | GID879 | aruD | succinylglutamate 5-semialdehyde dehydrogenase | PA14_52670 | PA0898 | aruD | 1.53 | 0.1 | 2 | Amino acid biosynthesis and metabolism
[NAME] | PA5203 | gshA | PAMr_nr_mas_10_3 | C2 | 42600 | correct | GID672 | gshA | glutamate--cysteine ligase | [NAME] | PA5203 | gshA | 1.50263874738002 | 0.163949869874402 | 4 | Biosynthesis of cofactors, prosthetic groups and carriers
PA14_38440 | PA2015 | PA2015 | PAMr_nr_mas_08_3 | E8 | 38983 | correct | GID1609 |  | putative isovaleryl-CoA dehydrogenase | PA14_38440 | PA2015 | PA2015 | 1.48466893050865 | 0.102282772068247 | 13 | Fatty acid and phospholipid metabolism
PA14_05960 | PA0456 | cspB | PAMr_nr_mas_07_2 | D5 | 36116 | correct | GID5392 | cspB | putative major cold shock protein | PA14_05960 | PA0456 | cspB | 1.47754483737351 | 0.0493053362912686 | 25 | Transcriptional regulators
PA14_53950 | PA0795 | prpC | PAMr_nr_mas_09_3 | G9 | 41035 | correct | GID1675 | prpC | citrate synthase 2 | PA14_53950 | PA0795 | prpC | 1.4516335407239 | 0.0352839275807952 | 5 | Carbon compound catabolism
PA14_52260 | PA0928 | gacS | PAMr_nr_mas_15_2 | H9 | 42741 | correct | GID113 | gacS | sensor/response regulator hybrid | PA14_52260 | PA0928 | gacS | 1.45 | 0.29 | 28 | Two-component regulatory systems
PA14_45940 | PA1432 | lasI | PAMr_nr_mas_08_4 | B10 | 39292 | correct | GID3828 | lasI | autoinducer synthesis protein LasI | PA14_45940 | PA1432 | lasI | 1.43975580686736 | 0.0483923977811543 | 1 | Adaptation, protection
PA14_09320 | PA4222 | pchI | PAMr_nr_mas_07_1 | E2 | 35711 | correct | GID624 | pchI | putative ATP-binding component of ABC transporter | PA14_09320 | PA4222 | pchI | 1.43765724325169 | 0.08056785615446539 | 27 | Transport of small molecules
PA14_52690 | PA0897 | aruG | PAMr_nr_mas_01_3 | B11 | 24118 | correct | GID2024 | aruG | arginine/ornithine succinyltransferase AII subunit | PA14_52690 | PA0897 | aruG | 1.43 | 0.14 | 2 | Amino acid biosynthesis and metabolism
PA14_25690 | PA2965 | fabF1 | PAMr_nr_mas_06_2 | E4 | 34095 | correct | GID1367 | fabF1 | beta-ketoacyl-acyl carrier protein synthase II | PA14_25690 | PA2965 | fabF1 | 1.42735371008913 | 0.0510892586386926 | 13 | Fatty acid and phospholipid metabolism
PA14_30230 | PA2620 | clpA | PAMr_nr_mas_08_4 | C11 | 39351 | correct | GID247 | clpA | ATP-dependent clp protease, ATP-binding subunit ClpA | PA14_30230 | PA2620 | clpA | 1.40977230364556 | 0.064005128053103 | 26 | Translation, post-translational modification, degradation
PA14_52660 | PA0899 | aruB | PAMr_nr_mas_05_3 | E5 | 32331 | correct | GID1065 | aruB | succinylarginine dihydrolase | PA14_52660 | PA0899 | aruB | 1.39 | 0.09 | 2 | Amino acid biosynthesis and metabolism
PA14_41730 | PA1766 | PA1766 | PAMr_nr_mas_13_4 | H4 | 55833 | correct | GID2087 |  | conserved hypothetical protein | PA14_41730 | PA1766 | PA1766 | 1.38318080682594 | 0.0139414953323338 | 14 | Hypothetical, unclassified, unknown
PA14_48590 | PA1216 | PA1216 | PAMr_nr_mas_12_2 | G3 | 47923 | correct | GID3100 |  | conserved hypothetical protein | PA14_48590 | PA1216 | PA1216 | 1.37547787054578 | 0.102678921794136 | 14 | Hypothetical, unclassified, unknown
PA14_51380 | PA1000 | pqsE | PAMr_nr_mas_11_3 | C6 | 45262 | correct | [NAME] | pqsE | Quinolone signal response protein | PA14_51380 | PA1000 | pqsE | 1.35928507596068 | 0.0273815907059835 | 21 | Quinolone signal response
PA14_43350 | PA1636 | kdpD | PAMr_nr_mas_05_2 | A3 | 31640 | correct | GID134 | kdpD | two-component sensor KdpD | PA14_43350 | PA1636 | kdpD | 1.35 | 0.01 | 28 | Two-component regulatory systems
PA14_41710 | PA1767 | PA1767 | PAMr_nr_mas_13_2 | A2 | 53876 | ND* | GID791 |  | putative membrane protein | PA14_41710 | PA1767 | PA1767 | 1.34202228070132 | 0.08101508645671429 | 15 | Membrane proteins
PA14_53940 | PA0796 | prpB | PAMr_nr_mas_08_3 | [NAME] | 39064 | correct | GID2652 | prpB | carboxyphosphonoenolpyruvate phosphonomutase | PA14_53940 | PA0796 | prpB | 1.34 | 0.08 | 5 | Carbon compound catabolism
PA14_42950 | PA1665 | fha2 | PAMr_nr_mas_02_1 | H8 | 25708 | correct | GID1569 | fha2 | conserved hypothetical protein | PA14_42950 | PA1665 | fha2 | 1.29878731106044 | 0.0643411805596783 | 14 | Hypothetical, unclassified, unknown
PA14_05310 | PA0407 | gshB | PAMr_nr_mas_10_3 | E9 | 42799 | correct | GID2256 | gshB | glutathione synthetase | PA14_05310 | PA0407 | gshB | 1.28 | 0.03 | 4 | Biosynthesis of cofactors, prosthetic groups and carriers
PA14_52720 | PA0895 | aruC | PAMr_nr_mas_14_2 | C8 | 56790 | correct | [NAME] | aruC | N-succinylglutamate 5-semialdehyde dehydrogenase | PA14_52720 | PA0895 | aruC | 1.24 | 0.21 | 2 | Amino acid biosynthesis and metabolism
[NAME] |  | wbpL | PAMr_nr_mas_07_1 | G5 | 35855 | correct | GID3451 | wbpL | putative glycosyltransferase L | [NAME] |  | wbpL | 1.23428742884453 | 0.0998746249892389 | 7 | Cell wall / LPS / capsule
PA14_14850 | PA3805 | pilF | PAMr_nr_mas_02_4 | H5 | 27116 | correct | [NAME] | pilF | type 4 fimbrial biogenesis protein PilF | PA14_14850 | PA3805 | pilF | 1.18 | 0.06 | 16 | Motility & Attachment
PA14_23430 |  | ORF_11 | PAMr_nr_mas_01_2 | E4 | 23782 | correct | GID6268 | ORF_11 | O antigen synhesis | PA14_23430 |  | ORF_11 | 1.17423688966058 | 0.149198953393506 | 7 | Cell wall / LPS / capsule
 |  |  | *another allele (mutantID #38780) with the same phenotype was sequenced