Spreadsheet: 00106cd7-b1a1-486f-afff-af9de1491bbe
Sheet: Table D.2 - Enzy Perf & Cost

[NAME] |  |  | PROPRIETARY/BUSINESS SENSITIVE
CE=(EL*EP/Y/BN)
EP=(BP+PP)*TB/P / (1-GM)
Y=(FC*CC*CG/RC + FH*CH*CX/RH) / (FC+FH)
Table B:  Enzyme Performance & Cost Contribution |  |  | Benchmark | Intermediate Target | Final Target |  | To calculate [NAME] or bioproduct per gram cellulose:
Applicant will need to substitute correct values for biofuel or bioproduct chosen, viz. density of chosen biofuel rather than that shown for ethanol. |  | % Improvement in cost contribution from benchmark | ----- |  |  |  | Fc      x | Cc          x | 1.111      x | CG       x | 0.5119     x | .001   x | 1.2674   x | 0.2642
CE | Enzyme Cost | $/gal Biofuel or $/lb bioproduct |  |  |  |  | g cellulose | g cellulose converted | [NAME] | g glucose converted | [NAME] or bioproduct | 1 kg | 1 L Biofuel or bioproduct | gallon
EP | Enzyme Price | $/L-product |  |  |  |  | g feed | g cellulose | g cellulose | [NAME] | [NAME] | 1000 g | 0.789 kg ethanol (modify for biofuel or product chosen) | 3.785 L
EL | Enzyme Loading (7 day residence time) | g protein/ (g cellulose + g xylan entering enzymatic hydrolysis)
BP | Broth Price | $/L-broth
PP | Post-ferm Treatment Cost | $/L-broth
TB/P | Broth-to-Product Ratio | L-broth/L-product
GM | Manufacturer's Gross Profit Margin | %
BN | Enzyme Concentration | g protein/ L-product |  |  |  |  | To calculate [NAME] or bioproduct per gram xylan:
Y | Biofuel or bioproduct Process Yield | gal [NAME] or g bioproduct/ (g cellulose + g xylan in raw feedstock) |  |  |  |  | FH     x | CH         x | 1.136      x | Cx      x | 0.5119     x | .001   x | 1.2674   x | 0.2642
 | Biofuel or bioproduct Process Yield | gal [NAME] or g bioproduct/ ton biomass |  |  |  |  | [NAME] | [NAME] converted | g xylose | g xylose converted | [NAME] or bioproduct | 1 kg | 1 L Biofuel or bioproduct | gallon
FC | Cellulose Fraction in Raw Feedstock | [NAME] |  |  |  |  | g feed | [NAME] | [NAME] | g xylose | g xylose | 1000 g | 0.789 kg ethanol (modify for biofuel or product chosen) | 3.785 L
FH | [NAME] in Raw Feedstock | [NAME]
CC | Cellulose Conversion to [NAME]  (including pretreatment conversion) | g cellulose converted/ g cellulose total | 0.9
CH | [NAME] to [NAME] (including pretreatment conversion) | [NAME] converted/ g xylan total | 0.9 |  |  |  | To calculate yield YT ([NAME] or bioproduct per ton biomass) from yield Y ([NAME] or bioproduct per g [NAME]):
CG | [NAME] to Biofuel or bioproduct | [NAME] | 0.95 |  |  |  | Y       x | (Fc + FH)         x | 1000          x | .4535      x | 2000
CX | Xylose Conversion to Biofuel or bioproduct | g xylose converted/ g xylose total | 0.85 |  |  |  |  | g cellulose + xylan | 1000 g | [NAME] | 2000 lb
RC | Theoretical Cellulose per [NAME] or bioproduct | [NAME] or lb bioproduct | 5250 |  |  |  |  | g biomass | [NAME] | 2.205 lb | ton
RH | Theoretical Xylan per [NAME] or bioproduct | [NAME] or lb bioproduct | 5135
Table B-ex:  Enzyme Performance & Cost Contribution Example |  |  | Benchmark | Intermediate Target | Final Target
 |  | % Improvement in cost contribution from benchmark | -----
CE | Enzyme Cost | $/gal Biofuel or lb bioproduct
EP | Enzyme Price | $/L or lb-product | 0.5
EL | Enzyme Loading (7 day residence time) | g protein/ (g cellulose + g xylan entering enzymatic hydrolysis) |  |  | 0.0045
BP | Broth Price | $/L-broth
PP | Post-ferm Treatment Cost | $/L-broth
TB/P | Broth-to-Product Ratio | L-broth/L-product
GM | Manufacturer's Gross Profit Margin | %
BN | Enzyme Concentration | g protein/ L or lb-product | 150
Y | Biofuel or bioproduct Process Yield | gal [NAME] or lb bioproduct/ (g cellulose + g xylan in raw feedstock)
 | Biofuel or bioproduct Process Yield | gal [NAME] or lb bioproduct/ ton biomass
FC | Cellulose Fraction in Raw Feedstock | [NAME] | 0.374
FH | [NAME] in Raw Feedstock | [NAME] | 0.211
CC | Cellulose Conversion to [NAME]  (including pretreatment conversion) | g cellulose converted/ g cellulose total | 0.9
CH | [NAME] to [NAME] (including pretreatment conversion) | [NAME] converted/ g xylan total | 0.9
CG | [NAME] to Biofuel or bioproduct | [NAME] | 0.95
CX | Xylose Conversion to Biofuel or bioproduct | g xylose converted/ g xylose total | 0.85
RC | Theoretical Cellulose per [NAME] or bioproduct | [NAME] or lb of bioproduct | 5250
RH | Theoretical Xylan per [NAME] or bioproduct | [NAME] or lb bioproduct | 5135
 |  |  | J0507B, roughly | SWAG | J0601A with only glucose and xylose fermented and no contamination losses