Spreadsheet: 12ad85d6-e1d0-4b22-a806-a142a0689d56
Sheet: Sheet1

Table S7: Overlap with related RNAi screens in Drosophila and human systems | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5
Table legend: Novel modifier genes not previously implicated in Glioblastoma are highlighted in green (suppressors) and red (enhancers). *Drosophila genes that did not emerge from the annotated RNAi screens, but that have well established roles in RTK and PI3K signaling and/or cell proliferation and survival in vivo.
[NAME] name | CG number | human orthologs | dEGFRλ; dp110CAAX | hits in cell-based Drosophila RNAi screens | hits in GBM genomic studies, known genetic mutations, and GBM cell-based RNAi screens
For | CG10033 | PRKG1 , PRKG2 | SS | cell cycle kinome screen (Bettencourt-Dias et al., 2004)
[NAME]* | CG10079 | EGFR, ERBB2, ERBB3, ERBB4 | SS |  | EGFR: established gliomagenic gene (Furnari et al., 2007)
S6K | CG10539 | [NAME], RPS6KB2 | SS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006) | established genes in gliomagenic signaling pathways (Furnari et al., 2007)
Cdk8 | CG10572 | CDK8 | WS | cell cycle screen (Bjorklund et al., 2006) | hit in an shRNA screen on GBM cell lines U87MG and [NAME] (Wiedemeyer et al., 2010)
Eip63E | CG10579 | PFTK1, PFTK2 | S | cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006) | PFTK1: hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
Madm | CG1098 | NRBP1, NRBP2 | S
CG11221 | CG11221 | [NAME], [NAME], NUAK1 | S
Hippo | CG11228 | [NAME], MST2 | E | cell cycle kinome screen (Bettencourt-Dias et al., 2004) | hippo pathway components function in aggressive glioblastoma (Bhat et al., 2011)
CG11660 | CG11660 | RIOK1 | SS
CG11859 | CG11859 | RIOK2 | SS
Warts | CG12072 | LATS1, [NAME] | E | cell cycle kinome screen (Bettencourt-Dias et al., 2004), growth and viability screen ([NAME], 2004) | hippo pathway components function in aggressive glioblastoma (Bhat et al., 2011)
Rolled | CG12559 | [NAME], ERK2 | SS | MAPK screen (Friedman and [NAME], 2006) | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Tkv | CG14026 | ACVRL1, ACVR1, ACVR1B, ACVR1C, BMPR1A, BMPR1B, TGFBR1 | S | cell cycle kinome screen (Bettencourt-Dias et al., 2004) | GBM stem cell RNAi screen (Wurdak et al., 2010), U87 LN229 shRNA screen ([NAME] et al., 2010),
Tao-1 | CG14217 | TAOK1, TAOK2, TAOK3 | E | MAPK screen (Friedman and [NAME], 2006)
Ack | CG14992 | TNK1, TNK2 | S | cell cycle kinome screen ((Bettencourt-Dias et al., 2004)
Dsor | CG15793 | MAP2K1, MAP2K2 | SS | MAPK screen (Friedman and [NAME], 2006) | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Taf1 | CG17603 | TAF1, TAF1L | SS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004)
InR | CG18402 | IGF1R,  INSR, INSRR | S | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004) | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Sax | CG1891 | ACVRL1, ACVR1, ACVR1B, ACVR1C, BMPR1A, BMPR1B, TGFBR1 | WS | cell cycle screen (Bjorklund et al., 2006) | GBM stem cell RNAi screen (Wurdak et al., 2010), hit in an shRNA screen on GBM cell lines U87MG and [NAME] (Wiedemeyer et al., 2010, established gliomagenic signaling pathway (eg. Ikushima et al. 2009)
CK1alpha | CG2028 | CSNK1A1 | SS | cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006)
Phl/Raf | CG2845 | ARAF, BRAF, [NAME] | SS | MAPK screen (Friedman and [NAME], 2006) | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Ksr | CG2899 | KSR1, [NAME] | SS | MAPK screen (Friedman and [NAME], 2006) | [NAME]: hit in an shRNA screen on GBM cell lines U87MG and [NAME] (Wiedemeyer et al., 2010), established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Drak | CG32666 | STK17A/DRAK1, STK17B/DRAK2 | S
Smg1 | CG32743 | SMG1 | E |  | hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
Cdk7 | CG3319 | CDK7 | SS | genome-wide cell-cycle screen (Bjorklund et al., 2006)
Nipped-A | CG33554 | TRAPP | S | MAPK screen (Friedman and [NAME], 2006) | hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
[NAME] | CG34412 | TLK1, TLK2 | SS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004)
Akt1 | CG4006 | AKT1, AKT2, AKT3 | SS | MAPK screen (Friedman and [NAME], 2006),            cell cycle screen (Bjorklund et al., 2006) | established gliomagenic gene (Furnari et al., 2007)
dp110* (PI3K92E) | CG4141 | PIK3CA (p110alpha), [NAME] (p110beta), PIK3CD (p110delta), [NAME] (p110gamma) | SS |  | established gliomagenic gene (Furnari et al., 2007)
CG42366 | CG42366 | MAK | SS | cell cycle screen (Bjorklund et al., 2006)
Pitslre | CG4268 | CDK11B, CDK11A | E | cell cycle screen (Bjorklund et al., 2006)
Cdk4 | CG5072 | CDK4, [NAME] | WS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004),   cell cycle screen (Bjorklund et al., 2006),               growth and viability screen ([NAME], 2004) | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Tor | CG5092 | MTOR | SS | MAPK screen (Friedman and [NAME], 2006),             cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006), | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Cdk9 | CG5179 | [NAME] | SS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006)
Cdc2 | CG5363 | [NAME] | S | MAPK screen (Friedman and [NAME], 2006), cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006) | established gene in gliomagenic signaling pathways (Furnari et al., 2007)
JIL-1 | CG6297 | RPS6KA5, RPS6KA4 | WS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004) | RPS6KA4: hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
CG6498 | CG6498 | MAST1, MAST2, MAST4, MAST3 | E | MAPK screen (Friedman and [NAME], 2006),             cell cycle kinome screen (Bettencourt-Dias et al., 2004) | MAST3: hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
Ial (auroraB) | CG6620 | AURKB, AURKA | S | cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006) | AURKB: expressed in aggressive GBM ([NAME], 2007)
Cdk12 | CG7597 | CDK12 | SS | MAPK screen (Friedman and [NAME], 2006),            cell cycle kinome screen (Bettencourt-Dias et al., 2004), cell cycle screen (Bjorklund et al., 2006)
Fray | CG7693 | STK39/SPAK, OXSR1 | S | cell cycle kinome screen (Bettencourt-Dias et al., 2004) | OXSR1, STK39: hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
[NAME] | CG7826 | DYRK1A, DYRK1B | WS | cell cycle kinome screen (Bettencourt-Dias et al., 2004) | DYRK1A: hit in a GBM stem cell RNAi screen (Wurdak et al., 2010)
Src42A | CG7873 | SRC, YES, FYN, LYN, HCK, LCK | SS | MAPK screen (Friedman and [NAME], 2006) | LCK: hit in an shRNA screen on GBM cell lines U87MG and [NAME] (Wiedemeyer et al., 2010),  FYN: hit in a GBM stem cell RNAi screen (Wurdak et al., 2010), established gene in gliomagenic signaling pathways (Furnari et al., 2007)
Punt* | CG7904 | ACVR2A, ACVR2B, BMPR2, AMHR2, [NAME] | S |  | established gliomagenic signaling pathway (eg. Ikushima et al., 2009)
[NAME] | CG8222 | PDGFRalpha, PDGFRbeta, KDR, FLT4, FLT1 | S | MAPK screen (Friedman and [NAME], 2006),            cell cycle screen (Bjorklund et al., 2006),               growth and viability screen ([NAME], 2004) | PDGFRalpha: established gliomagenic gene (Furnari et al., 2007), GBM stem cell RNAi screen (Wurdak et al., 2010)
CG8878 | CG8878 | VRK1, VRK2 | SS | MAPK screen (Friedman and [NAME], 2006) | VRK2: hit in an shRNA screen on GBM cell lines U87MG and [NAME] (Wiedemeyer et al., 2010)
References
Bettencourt-Dias, M., Giet, R., Sinka, R., Mazumdar, A., Lock, W.G., Balloux, [NAME], Zafiropoulos, P.J., Yamaguchi, S., Winter, S., Carthew, R.W., et [NAME]. (2004). Genome-wide survey of protein kinases required for cell cycle progression. Nature 432, 980-987.
Bhat K.P., Salazar [NAME], Balasubramaniyan V., [NAME], [NAME], Hollingsworth F., [NAME], [NAME], [NAME], [NAME], [NAME], [NAME], [NAME], [NAME], [NAME], [NAME], Lang F.F., [NAME], [NAME], [NAME], Aldape K.D. (2011) The transcriptional coactivator TAZ regulates mesenchymal differentiation in malignant glioma. Genes Dev 25, 2594-609.
Bjorklund, M., Taipale, M., Varjosalo, M., [NAME], [NAME], Lahdenpera, J., and Taipale, J. (2006). Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439, 1009-1013.
Boutros, M., Kiger, A.A., Armknecht, S., Kerr, K., Hild, M., [NAME], [NAME], Haas, S.A., Paro, R., and [NAME], N. (2004). Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303, 832-835.
Furnari, F.B., Fenton, T., Bachoo, R.M., Mukasa, A., Stommel, [NAME], Stegh, A., Hahn, W.C., Ligon, K.L., Louis, D.N., Brennan, C., et [NAME]. (2007). Malignant astrocytic glioma: genetics, biology, and paths to treatment. Genes Dev 21, 2683-2710.
Friedman, A., and [NAME], N. (2006). A functional RNAi screen for regulators of receptor [NAME] and ERK signalling. Nature 444, 230-234.
[NAME], [NAME], [NAME], [NAME], [NAME], [NAME], (2009). Autocrine TGF-beta signaling maintains tumorigenicity of glioma-initiating cells through Sry-related HMG-box factors. Cell Stem Cell 5, 504-14
Wiedemeyer, W.R., Dunn, I.F., [NAME], S.N., Zhang, J., Chheda, M.G., Dunn, G.P., Zhuang, [NAME], [NAME], [NAME], [NAME], S., Xiao, [NAME], et [NAME]. (2010). Pattern of retinoblastoma pathway inactivation dictates response to CDK4/6 inhibition in GBM. Proc Natl Acad Sci U S A 107, 11501-11506.
Wurdak, H., Zhu, S., [NAME], [NAME], [NAME], M., Watson, J., Chiang, C.Y., Zhang, J., Natu, V.S., Lairson, L.L., Walker, J.R., et [NAME]. (2010). An RNAi screen identifies TRRAP as a regulator of brain tumor-initiating cell differentiation. Cell Stem Cell 6, 37-47.
[NAME], W.F., Navaratne, K., Prayson, R.A., Weil, [NAME] (2007). [NAME] expression correlated with aggressive behaviour in glioblastoma multiforme. J [NAME], 218-21.