Spreadsheet: 23453e0f-5e9f-4c5e-902a-a75555f0fa4e
Sheet: Ung-

mutant number | mutated gene | position in Chr. V (distance from telomere) | distance between adjacent mutations | >1 mutation in gene? | WT base(s) | mutant base(s) | deletion/insertion (-/+ number of bases) | 5′ flanking sequence | 3′ flanking sequence | type of mutation
027-048-BA01A | 3′ LYS2 | 2900 | 791 |  | G | C |  | TGTACTCAGCAGCCCATTTG | ACTGACCATATCCACCAGTG | substitution
 | ADE2 | 3691 | 2758 |  | G | C |  | ATGTGCTAACGATTGAGATT | AGCATGTTGATGTTCCTACA | substitution
 | URA3 | 6449 | 1186 |  | G | A |  | ACTAGGATGAGTAGCAGCAC | TTCCTTATATGTAGCTTTCG | substitution
 | CAN1 | 7635 | 143 | + | G | A |  | TTTCCCGACGAGAGTAAATG | CGAGGATACGTTCTCTATGG | substitution
 | CAN1 | 7778 | 354 | + | G | A |  | [NAME] | GTTTATCCACACCTCTGACC | substitution
 | CAN1 | 8132 | 240 | + | G | A |  | TCAAATATTACGGTGAATTC | AGTTCTGGGTCGCTTCCATC | substitution
 | CAN1 | 8372 |  | + | GC | AG |  | CTGAACTAGTTGGTATCACT | TGGTGAAGCTGCAAACCCCA | complex
027-048-BA01B | 3′ LYS2 | 2657 | 2378 |  | G | A |  | [NAME] | GAGGATTCAAAGACGTAGCA | substitution
 | ADE2 | 5035 | 1197 |  | G | A |  | CTATTAATAATAGTACGAAC | CTGCGCTGTTGGCTGTCAGA | substitution
 | URA3 | 6232 | 189 | + | G | A |  | GTAAAAAATTGTACTTGGCG | ATAATGCCTTTAGCGGCTTA | substitution
 | URA3 | 6421 | 1563 | + | G | A |  | TGATATTAAATAGCTTGGCA | CAACAGGACTAGGATGAGTA | substitution
 | CAN1 | 7984 |  |  | G | A |  | TACATGTATTGGTTTTCTTG | GCAATCACTTTTGCCCTGGA | substitution
027-048-BA03 | ADE2 | 4596 | 329 | + | G | C |  | ATGGCGGAATGTGAACAAAG | CTGAACTACATTACAGGTAG | substitution
 | ADE2 | 4925 | 944 | + | G | A |  | GACACCACTTCCTGTCATCG | TGTGCCCGTAAAAGGTTCTT | substitution
 | URA3 | 5869 | 83 | + | G | A |  | TGTCATCTAAACCCACACCG | GTGTCATAATCAACCAATCG | substitution
 | URA3 | 5952 | 315 | + | G | A |  | CCGATAACAAAATCTTTGTC | CTCTTCGCAATGTCAACAGT | substitution
 | URA3 | 6267 | 1539 | + | G | A |  | GGCTTAACTGTGCCCTCCAT | GAAAAATCAGTCAAGATATC | substitution
 | CAN1 | 7806 | 149 | + | G | A |  | CACACCTCTGACCAACGCCG | CCCAGTGGGCGCTCTTATAT | substitution
 | CAN1 | 7955 | 757 | + | G | A |  | TTTCTCCAGCATTTGGTGCG | CCAATGGTTACATGTATTGG | substitution
 | CAN1 | 8712 |  | + | G | A |  | CCTGTCAAGGACCACCAAAG | TGGTGTTCCATACATTGCAG | substitution
027-048-BA04 | 3′ LYS2 | 2633 | 2153 |  | G | A |  | TTGGGTGACCCGTTACTTGA | CAACGGCCAATTCATTTTCT | substitution
 | ADE2 | 4786 | 1276 |  | G | A |  | TTGAAGTGACAATAGTCTCT | CTCATAGAACTCCACATAGG | substitution
 | URA3 | 6062 | 3052 |  | CGTC | AGTA |  | AGGTTCCTTTATTACTTCTT | CGCCTGCTTCAAACCGCTAA | complex
 | CAN1 | 9114 |  |  | G | A |  | ATTCAGATGCAGATTTATTT | GAAGATTGGAGATGTCGACA | substitution
027-048-BA06A | ADE2 | 4355 | 345 | + | G | A |  | TCAATTTGAAGCTCATTTGA | ATCAATATTGGATTTGCCAA | substitution
 | ADE2 | 4700 | 138 | + | G | A |  | ATTGGTTGGAATCATCATGG | ATCAGACTCTGACTTGCCGG | substitution
 | ADE2 | 4838 | 1316 | + | G | A |  | TGCTATTTCCGCAAGCAAGC | TGG-AATTAAAACAATTATC | substitution
 | URA3 | 6154 | 36 | + | G | A |  | CTGCTATTCTGTATACACCC | CAGAGTACTGCAATTTGACT | substitution
 | URA3 | 6190 | 1559 | + | G | T |  | TGACTGTACTACCAATGTCA | CAAATTTTCTGTCTTCGAAG | substitution
 | CAN1 | 7749 |  |  | G | A |  | TATTGGTATGATTGCCCTTG | TGGTACTATTGGTACAGGTC | substitution
027-048-BA06B | 3′ LYS2 | 2216 | 70 | + | G | A |  | TTCCACGAGCACCAGCACCT | AAGCAACTAGACTTATTTGC | substitution
 | 3′ LYS2 | 2286 | 2263 | + | G | A |  | AAGTTTGTCATTATGAGTTG | TGGAGGTAAAAATCCAACCT | substitution
 | ADE2 | 4549 | 1840 |  | G | C |  | [NAME] | GTCACATAAATATTATTGCC | substitution
 | URA3 | 6389 | 1378 |  | G | A |  | TGAAGCACACAAGTTTGTTT | CTTTTCGTGCATGATATTAA | substitution
 | CAN1 | 7767 |  |  | G | A |  | TGGTGGTACTATTGGTACAG | TCTTTTCATTGGTTTATCCA | substitution
027-048-BA07 | 3′ LYS2 | 2658 | 1573 |  | G | A |  | GGCCAATTCATTTTCTTTGG | AGGATTCAAAGACGTAGCAA | substitution
 | ADE2 | 4231 | 72 | + | G | A |  | TTCCCGGTTGTGGTATATTT | GTGTGGAAATGTTCTATTTA | substitution
 | ADE2 | 4303 | 1586 | + | G | A |  | CCCCAAGGCCTCACAACTCT | GACATTATACCATTGATGCT | substitution
 | URA3 | 5889 | 496 | + | G | A |  | GGTGTCATAATCAACCAATC | TAACCTTCATCTCTTCCACC | substitution
 | URA3 | 6385 | 1617 | + | G | A |  | CCAATGAAGCACACAAGTTT | TTTGCTTTTCGTGCATGATA | substitution
 | CAN1 | 8002 | 409 | + | G | A |  | TGGGCAATCACTTTTGCCCT | GAACTTAGTGTAGTTGGCCA | substitution
 | CAN1 | 8411 | 467 | + | G | A |  | CCAGAAAATCCGTTCCAAGA | CCATCAAAAAAGTTGTTTTC | substitution
 | CAN1 | 8878 |  | + | G | A |  | ATCTCGCACATCAGATTTAT | CAAGCTTTGAAATACCGTGG | substitution
027-048-BA08 | ADE2 | 4889 | 1500 |  | G | A |  | TGGGGCTGCTCACTTGCCAG | TATGGTGGCTGCAATGACAC | substitution
 | URA3 | 6389 | 1767 |  | G | A |  | TGAAGCACACAAGTTTGTTT | CTTTTCGTGCATGATATTAA | substitution
 | CAN1 | 8156 |  |  | GTTTTA- | CTTTTTG | +1 | TCTGGGTCGCTTCCATCAAA | [NAME] | complex
027-048-BA09 | ADE2 | 4217 | 41 | + | G | A |  | TGCAATCAAATCTTTTCCCG | TTGTGGTATATTTGGTGTGG | substitution
 | ADE2 | 4258 | 1755 | + | G | A |  | AAATGTTCTATTTAGAAACA | GGGAATTGCTTATTAACGAA | substitution
 | URA3 | 6013 | 2110 |  | G | A |  | TAGATAGGGAGCCCTTGCAT | ACAATTCTGCTAACATCAAA | substitution
 | CAN1 | 8123 |  |  | G | A |  | TGTTCCCTGTCAAATATTAC | GTGAATTCGAGTTCTGGGTC | substitution
027-048-BA10 | 3′ LYS2 | 2235 | 246 | + | G | C |  | TGAAGCAACTAGACTTATTT | CGCTTGAGTTAGTTCTATAC | substitution
 | 3′ LYS2 | 2481 | 1537 | + | G | A |  | TAAGACCATGTGTAGCAAAG | ATACAACGCATTTTCTTCAT | substitution
 | ADE2 | 4018 | 81 | + | G | A |  | AGGATCGTCCTTTGTACGCC | AAAAATGGGCACCATTTACT | substitution
 | ADE2 | 4099 | 2011 | + | G | A |  | TAGTGTTTTCTTACCCAATT | TAGAGACTATCCACAAGGAC | substitution
 | URA3 | 6110 | 275 | + | G | A |  | ACCTGGACCCACCACACCGT | TGCATTCGTAATGTCTGCCC | substitution
 | URA3 | 6385 | 1375 | + | G | A |  | CCAATGAAGCACACAAGTTT | TTTGCTTTTCGTGCATGATA | substitution
 | CAN1 | 7760 |  |  | G | C |  | TTGCCCTTGGTGGTACTATT | GTACAGGTCTTTTCATTGGT | substitution
027-048-BA13 | ADE2 | 3531 | 610 | + | G | A |  | TTGGGACGTATGATTGTTGA | GCAGCAAACAGGCTCAACAT | substitution
 | ADE2 | 4141 | 558 | + | G | C |  | ATATTTGTGACTTATGTTAT | CGCCTGCTAGAGTTCCGGAC | substitution
 | ADE2 | 4699 | 456 | + | G | A |  | CATTGGTTGGAATCATCATG | GATCAGACTCTGACTTGCCG | substitution
 | ADE2 | 5155 | 636 | + | G | A |  | AAGCACAAAAGTTAGAAACT | TCGGTTACGAAGCTTATCTA | substitution
 | URA3 | 5791 | 2349 |  | G | A |  | TTCCAACAATAATAATGTCA | ATCCTGTAGAGACCACATCA | substitution
 | CAN1 | 8140 |  |  | G | A |  | TACGGTGAATTCGAGTTCTG | GTCGCTTCCATCAAAGTTTT | substitution
027-048-BA14A | ADE2 | 4189 | 2023 |  | G | A |  | AACTTAAGGCGAAGTTGTTG | CAGAAAATGCAATCAAATCT | substitution
 | URA3 | 6212 | 17 | + | GT- | ATA | +1 | AAATTTTCTGTCTTCGAAGA | AAAAAATTGTACTTGGCGGA | complex
 | URA3 | 6229 | 1850 | + | G | A |  | AGAGTAAAAAATTGTACTTG | CGGATAATGCCTTTAGCGGC | substitution
 | CAN1 | 8079 |  |  | G | A |  | GGCATGGATTAGTATTTTTT | GGTAATTATCACAATAATGA | substitution
027-048-BA14B | 3′ LYS2 | 2779 | 1524 |  | G | A |  | CCTTTCAAAAATCTCAATAA | AAATCATCTGTGTTTGAAGA | substitution
 | ADE2 | 4303 | 1710 |  | G | A |  | CCCCAAGGCCTCACAACTCT | GACATTATACCATTGATGCT | substitution
 | URA3 | 6013 | 1971 |  | G | A |  | TAGATAGGGAGCCCTTGCAT | ACAATTCTGCTAACATCAAA | substitution
 | CAN1 | 7984 |  |  | G | A |  | TACATGTATTGGTTTTCTTG | GCAATCACTTTTGCCCTGGA | substitution
027-048-BA14C | ADE2 | 5060 | 976 |  | T | G |  | GCTGTTGGCTGTCAGACTGC | TGGCGCTTATGATTCAAGTT | substitution
 | URA3 | 6036 | 49 | + | G | A |  | AATTCTGCTAACATCAAAAG | CCTCTAGGTTCCTTTATTAC | substitution
 | URA3 | 6085 | 375 | + | A | C |  | CCGCCTGCTTCAAACCGCTA | CAATACCTGGACCCACCACA | substitution
 | URA3 | 6460 | 1524 | + | G | A |  | TAGCAGCACGTTCCTTATAT | TAGCTTTCGACATGATTTAT | substitution
 | CAN1 | 7984 |  |  | G | A |  | TACATGTATTGGTTTTCTTG | GCAATCACTTTTGCCCTGGA | substitution
027-048-BA15 | ADE2 | 4014 | 1084 | + | C | G |  | CTGAAGGATCGTCCTTTGTA | GCCGAAAAATGGGCACCATT | substitution
 | ADE2 | 5098 | 1195 | + | C | A |  | GTTATACAACGAAAATGGAA | AGTTTTTATTAAAGCAAGAA | substitution
 | URA3 | 6293 | 1467 |  | G | A |  | ATCAGTCAAGATATCCACAT | TGTTTTTAGTAAACAAATTT | substitution
 | CAN1 | 7760 |  |  | G | A |  | TTGCCCTTGGTGGTACTATT | GTACAGGTCTTTTCATTGGT | substitution
028-048-CA1A | 3′ LYS2 | 2554 | 265 | + | G | C |  | TTCCATTTAGAATAGCTTTC | ATTTCGACATCGTAACCATA | substitution
 | 3′ LYS2 | 2819 | 866 | + | G | A |  | AACCATTGGCAGAGGCACCT | TTACGTAACCTGGTCTGACA | substitution
 | ADE2 | 3685 | 2590 |  | G | A |  | AATGTGATGTGCTAACGATT | AGATTGAGCATGTTGATGTT | substitution
 | URA3 | 6275 | 256 | + | C | A |  | TGTGCCCTCCATGGAAAAAT | AGTCAAGATATCCACATGTG | substitution
 | URA3 | 6531 | 1601 | + | G | A |  | AGTTGGGTTAAGAATACTGG | CAATTTCATGTTTCTTCAAC | substitution
 | CAN1 | 8132 |  |  | G | A |  | TCAAATATTACGGTGAATTC | AGTTCTGGGTCGCTTCCATC | substitution
028-048-CA3A | ADE2 | 3804 | 385 | + | G | A |  | [NAME] | CATTTAATCAAAAATGGTAT | substitution
 | ADE2 | 4189 | 927 | + | G | A |  | AACTTAAGGCGAAGTTGTTG | CAGAAAATGCAATCAAATCT | substitution
 | ADE2 | 5116 | 1177 | + | G | A |  | AACAGTTTTTATTAAAGCAA | AAGAAGAAGTTCTTGTCAAA | substitution
 | URA3 | 6293 | 1767 |  | G | A |  | ATCAGTCAAGATATCCACAT | TGTTTTTAGTAAACAAATTT | substitution
 | CAN1 | 8060 | 270 | + | G | A |  | CGTACAAAGTTCCACTGGCG | CATGGATTAGTATTTTTTGG | substitution
 | CAN1 | 8330 |  | + | G | C |  | GGGTTTCCTCTTTGATTAAC | CTGCCTTCACATTTCAAGGT | substitution
028-048-CA3B | ADE2 | 3515 | 289 | + | G | A |  | ATTAGGAGGGGGACAATTGG | ACGTATGATTGTTGAGGCAG | substitution
 | ADE2 | 3804 | 385 | + | G | A |  | [NAME] | CATTTAATCAAAAATGGTAT | substitution
 | ADE2 | 4189 | 927 | + | G | A |  | AACTTAAGGCGAAGTTGTTG | CAGAAAATGCAATCAAATCT | substitution
 | ADE2 | 5116 | 1177 | + | G | A |  | AACAGTTTTTATTAAAGCAA | AAGAAGAAGTTCTTGTCAAA | substitution
 | URA3 | 6293 | 1767 |  | G | A |  | ATCAGTCAAGATATCCACAT | TGTTTTTAGTAAACAAATTT | substitution
 | CAN1 | 8060 | 270 | + | G | A |  | CGTACAAAGTTCCACTGGCG | CATGGATTAGTATTTTTTGG | substitution
 | CAN1 | 8330 |  | + | G | C |  | GGGTTTCCTCTTTGATTAAC | CTGCCTTCACATTTCAAGGT | substitution
028-048-CA4 | ADE2 | 3928 | 534 | + | G | C |  | TCTTGAAGTCGAGGACTTTG | CATACGATGGAAGAGGTAAC | substitution
 | ADE2 | 4462 | 1833 | + | G | C |  | ACAAACATACAAAAGATAAA | [NAME] | substitution
 | URA3 | 6295 | 1967 |  | G | - | -1 | CAGTCAAGATATCCACATGT | TTTTTAGTAAACAAATTTTG | deletion
 | CAN1 | 8262 |  |  | G | A |  | GAGAAACCCAGGTGCCTGGG | TCCAGGTATAATATCTAAGG | substitution
028-048-CA7A | ADE2 | 4025 | 1740 |  | G | A |  | [NAME] | [NAME] | substitution
 | URA3 | 5765 | 503 | + | G | C |  | ATCCCTTCCCTTTGCAAATA | TCCTCTTCCAACAATAATAA | substitution
 | URA3 | 6268 | 2675 | + | G | A |  | GCTTAACTGTGCCCTCCATG | AAAAATCAGTCAAGATATCC | substitution
 | CAN1 | 8943 |  |  | G | A |  | TAGAGCTAAATTAATGCCCG | CTTGGCTTATTATGCGGCCA | substitution
028-048-CA7B | ADE2 | 5063 | 787 |  | G | A |  | GTTGGCTGTCAGACTGCTTG | [NAME] | substitution
 | URA3 | 5850 | 14 | + | G | C |  | TGACCCAATGCGTCTCCCTT | TCATCTAAACCCACACCGGG | substitution
 | URA3 | 5864 | 1941 | + | C | T |  | TCCCTTGTCATCTAAACCCA | [NAME] | substitution
 | CAN1 | 7805 | 400 | + | G | A |  | CCACACCTCTGACCAACGCC | GCCCAGTGGGCGCTCTTATA | substitution
 | CAN1 | 8205 |  | + | G | A |  | CTGTTTTTGTATGGTTTGTG | TGCTGGGGTTACCGGCCCAG | substitution
028-048-CA10 | ADE2 | 3786 | 1314 | + | C | G |  | ACAATCAGATTGATACAAGA | [NAME] | substitution
 | ADE2 | 5100 | 1132 | + | G | A |  | TATACAACGAAAATGGAACA | [NAME] | substitution
 | URA3 | 6232 | 1403 |  | G | A |  | GTAAAAAATTGTACTTGGCG | ATAATGCCTTTAGCGGCTTA | substitution
 | CAN1 | 7635 | 406 | + | G | A |  | TTTCCCGACGAGAGTAAATG | CGAGGATACGTTCTCTATGG | substitution
 | CAN1 | 8041 | 382 | + | G | A |  | CAAGTCATTCAATTTTGGAC | TACAAAGTTCCACTGGCGGC | substitution
 | CAN1 | 8423 | 459 | + | G | C |  | TTCCAAGAGCCATCAAAAAA | TTGTTTTCCGTATCTTAACC | substitution
 | CAN1 | 8882 |  | + | G | C |  | CGCACATCAGATTTATGCAA | CTTTGAAATACCGTGGCATC | substitution
028-048-CA12 | ADE2 | 3649 | 587 | + | GAAAAAC | -AAAAAA | -1 | TTTCCAATCCTCTTGATATC | [NAME] | complex
 | ADE2 | 4236 | 1835 | + | G | A |  | GGTTGTGGTATATTTGGTGT | GAAATGTTCTATTTAGAAAC | substitution
 | URA3 | 6071 | 1690 |  | G | A |  | TATTACTTCTTCTGCCGCCT | CTTCAAACCGCTAACAATAC | substitution
 | CAN1 | 7761 | 299 | + | G | A |  | TGCCCTTGGTGGTACTATTG | TACAGGTCTTTTCATTGGTT | substitution
 | CAN1 | 8060 | 112 | + | G | C |  | CGTACAAAGTTCCACTGGCG | CATGGATTAGTATTTTTTGG | substitution
 | CAN1 | 8172 | 508 | + | G | A |  | CAAAGTTTTAGCCATTATCG | GTTTCTAATATACTGTTTTT | substitution
 | CAN1 | 8680 | 291 | + | G | A |  | GGTCTATCAAAGAACAAGTT | GCTCCTAAATTCCTGTCAAG | substitution
 | CAN1 | 8971 |  | + | G | A |  | TATTATGCGGCCACATTTAT | ACGATCATTATCATTATTCA | substitution
028-048-CA13 | ADE2 | 5005 | 996 |  | G | C |  | TGCCTAGAGGTGTTCCAGTA | CTACCGTCGCTATTAATAAT | substitution
 | URA3 | 6001 | 1766 |  | G | A |  | TATATTCTCCAGTAGATAGG | AGCCCTTGCATGACAATTCT | substitution
 | CAN1 | 7767 | 545 | + | G | A |  | TGGTGGTACTATTGGTACAG | TCTTTTCATTGGTTTATCCA | substitution
 | CAN1 | 8312 |  | + | G | C |  | AAGGGAGGTTCTTAGGTTGG | TTTCCTCTTTGATTAACGCT | substitution