Spreadsheet: 01b01248-f970-4bb9-a306-2af4d9b813f7
Sheet: Sheet1

Supplemental Table S1. Identification of differentially expressed proteins of A. catenella CI01 at different toxin biosynthesis stages. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13
Spot ID a. | NCBI b | Protein name | Theoretical  pI | Theoretical  MW | No. of matched peptides | Sequence Coverage (%) | Protein score | Protein score C.I.% | Function | T28 vs T24 |  | T34 vs T24
 |  |  |  |  |  |  |  |  |  | Fold change c | P-Value | Fold change | P-Value
Protein modification
1 | 26991410 | chaperone protein DnaK | 4.83 | 68.87 | 10 | 38 | 88 | 98.32 | protein folding; response to stress | 0.46 | 0.031 | 1.2 | 0.102
3 | 215400773 | Hsp70-type chaperone | 5.04 | 68.81 | 8 | 45 | 137 | 100 | Heat shock protein 70 family; protein folding | 0.76 | 0.839 | 2.67 | 0.006
7 | 148549568 | [NAME] | 4.97 | 56.75 | 13 | 51 | 334 | 100 | Protein and ATP binding | 0.32 | 0.021 | 1.34 | 0.518
12 | 148549568 |  | 4.97 | 56.75 | 23 | 89 | 730 | 100 |  | 2.2 | 0.002 | 1.5 | 0.106
Metabolism
8 | 170723674 | [NAME] | 5.85 | 45.18 | 6 | 80 | 101 | 99.91 | L-serine,glycine,one-carbon metabolic process | 0.44 | 0.015 | 0.83 | 0.572
42 | 70732651 |  | 5.74 | 45.15 | 5 | 46 | 93 | 99.49 |  | 0.35 | 0.002 | 0.62 | 0.023
15 | 35210480 | glyceraldehyde-3-phosphate dehydrogenase | 4.81 | 26.21 | 1 | 63 | 248 | 95.24 | Glucose metabolic process;oxidation reduction | 1.2 | 0.621 | 2.06 | 0.003
16 | 35210480 |  | 4.81 | 26.21 | 1 | 77 | 248 | 100 |  | 2.21 | 0.093 | 3.67 | 0.003
17 | 35210480 |  | 4.81 | 26.21 | 1 | 31 | 101 | 100 |  | 0.3 | 0.241 | 2.45 | 0.018
83 | 35210480 |  | 4.81 | 26.21 | 1 | 89 | 248 | 100 |  | 0.49 | 0.015 | 0.25 | 0.002
19 | 58613475 | phosphoglycerate kinase | 6.16 | 27.32 | 1 | 48 | 271 | 100 | Glycolysis | 2.19 | 0.074 | 3.47 | 0.003
37 | 58613.455 | Chloroplast ferredoxin-NADP+ reductase | 4.78 | 28.67 | 2 | 90 | 389 | 100 | ferredoxin-NADP reductase type 1 family;Oxidation reduction | 0.13 | 0.007 | 1.19 | 0.641
40 | 170723385 | ornithine carbamoyltransferase | 5.92 | 38.1 | 15 | 68 | 296 | 100 | Cellular amino acid metabolic process | 0.45 | 0.088 | 1.61 | 0.003
31 | 46909371 | Methionine (S-) adenosyltransferase | 5.81 | 34.39 | 4 | 29 | 145 | 100 | Transferase; one-carbon metabolic | 1.26 | 0.45 | 0.4 | 0.043
32 | 158524698 |  | 5.59 | 35.3 | 3 | 73 | 149 | 100 |  | 1.89 | 0.173 | 0.47 | 0.033
33 | 71370920 |  | 6.82 | 35.08 | 3 | 77 | 202 | 100 |  | 1.15 | 0.767 | 0.4 | 0.034
34 | 225685869 |  | 5.54 | 45.52 | 3 | 90 | 121 | 100 |  | 0.92 | 0.942 | 0.46 | 0.042
36 | 225685865 |  | 5.73 | 51.08 | 4 | 93 | 92 | 99.248 |  | 2.5 | 0.007 | 0.18 | 0.091
41 | 104782658 | succinyl-CoA synthetase subunit beta | 5.83 | 41.47 | 3 | 39 | 92 | 99.27 | Tricarboxylic acid cycle | 0.32 | 0.024 | 1.55 | 0.058
45 | 134037072 | plastid oxygen-evolving enhancer 1–2 precursor | 5.37 | 27.84 | 1 | 95 | 333 | 97.61 | Photosynthesis; photosystemⅡ stabilization | 0.38 | 0.198 | 1.24 | 0.801
47 | 118577178 | Chorismatemutase/prephenatedehydratas | 6.6 | 25.75 | 7 | 70 | 85 | 96.79 | L-phenylalanine biosynthetic process | 0.48 | 0.135 | 3.11 | <0.001
49 | 149071995 | photosystem I assembly protein ycf1 | 9.79 | 26.39 | 2 | 85 | 80 | 100 | photosynthesis | 0.1 | 0.205 | 3.63 | 0.002
52 | 170723490 | nucleoside-diphosphate kinase | 5.23 | 15 | 7 | 85 | 298 | 100 | CTP,GTP,UTP biosynthetic process | 0.55 | 0.845 | 2.52 | 0.125
54 | 56693619 | protein kinase C conserved region 2 | 8.7 | 23.29 | 2 | 64 | 64.3 | 99.98 | Transferase;kinase activity | 0.92 | 0.995 | 2.12 | 0.073
55 | 26987276 | inorganic pyrophosphatase | 4.77 | 19.18 | 2 | 82 | 87 | 97.73 | Phosphate metabolic process | 1 | 1 | 11.55 | 0.003
56 | 3355306 | light-harvesting polyprotein precursor | 5.26 | 22.37 | 2 | 98 | 1671 | 100 | Photosynthesis,light harvesting | 0.46 | 0.008 | 0.54 | 0.018
67 | 219114000 | fructose-bisphosphate aldolase | 9.71 | 27.23 | 1 | 90 | 185 | 100 | Glycolysis | 2.18 | <0.001 | 1.06 | 0.947
69 | 71000213 | enolase 2 | 5.07 | 40.57 | 7 | 20 | 205 | 100 | glycolysis | 2.88 | 0.006 | 0.8 | 0.923
78 | 148546281 | arginine deiminase | 5.66 | 46.73 | 18 | 71 | 660 | 100 | arginine catabolic process; Inhibits Cell Proliferation; Arresting Cell Cycle and Inducing Apoptosis | 3.3 | <0.001 | 0.2 | 0.008
60 | 75284311 | ribulose 1,5-bisphosphate carboxylase, chloroplastic; | 7.01 | 28.88 | 2 | 95 | 476 | 99.99 | Oxidation reduction; photorespiration; reductive pentose-phosphate cycle | 0.46 | 0.072 | 0.87 | 0.861
68 | 75284311 |  | 7.01 | 28.88 | 2 | 94 | 476 | 99.99 |  | 2.14 | 0.005 | 1.02 | 1
75 | 37730371 |  | 6.26 | 60.21 | 6 | 94 | 95 | 99.64 |  | 2.52 | 0.008 | 0.81 | 0.903
71 | 75284311 |  | 7.01 | 28.89 | 1 | 97 | 476 | 99.43 |  | 8.11 | 0.001 | 1.08 | 1
70 | 37731771 | ribulose 1,5-bisphosphate carboxylase oxygenase form II (small subunit) | 6.09 | 34.03 | 5 | 95 | 144 | 100 | Oxidation reduction; photorespiration; reductive pentose-phosphate cycle | 0.37 | 0.298 | 2.21 | 0.037
72 | 33317821 | ribulose 1,5-bisphosphate carboxylase oxygenase large subunit | 5.66 | 50.47 | 5 | 95 | 89 | 98.57 | Oxidation reduction; photorespiration; reductive pentose-phosphate cycle | 3.05 | 0.022 | 1.32 | 0.901
73 | 33317821 |  | 5.66 | 50.47 | 4 | 93 | 135 | 100 |  | 2.96 | 0.048 | 1.13 | 0.996
74 | 33317821 |  | 5.66 | 50.47 | 4 | 95 | 135 | 100 |  | 3.36 | 0.11 | 1.07 | 1
76 | 211939908 | adenosylhomocysteinase (AdoHcy) | 5.74 | 27.97 | 8 | 79 | 150 | 100 | one-carbon metabolic process; an enzyme that converts SAH to homocysteine and adenosine | 2.2 | 0.003 | 0.49 | 0.117
79 | 211939908 |  | 5.74 | 27.97 | 7 | 83 | 116 | 99.99 |  | 3.32 | 0.003 | 0.88 | 0.985
77 | 211939908 | S-adenosylhomocysteinase (SAH) | 5.74 | 27.97 | 9 | 89 | 120 | 99.99 | one-carbon metabolic process; an intermediate in the synthesis of cysteine and formed by the demethylation of SAM | 2.89 | 0.001 | 0.86 | 0.905
82 | 257480821 | plastid protein NAP50 | 5.72 | 51.89 | 6 | 91 | 111 | 100 | Nitrogen-associated protein 50. | 0.99 | 1 | 2.2 | 0.011
88 | 257480821 |  | 5.72 | 51.89 | 7 | 75 | 138 | 100 |  | 1.1 | 0.991 | 2.26 | 0.051
89 | 257480821 |  | 5.72 | 51.89 | 7 | 75 | 90 | 98.91 |  | 2.02 | 0.073 | 1.33 | 0.735
93 | 27450755 | chlorophyll A–C binding protein | 5.26 | 22.37 | 2 | 69 | 183 | 100 | photosynthesis | 0.39 | 0.102 | 1.78 | 0.041
94 | 115901552 | similar to sulfotransferase (SULT) | 4.7 | 35.22 | 1 | 88 | 122 | 96.24 | sulfotransferase activity | 0.44 | 0.043 | 1.24 | 0.459
Cell cycle regulation
9 | 163750818 | Cell division protein FtsH | 4.89 | 71.9 | 10 | 64 | 135 | 100 | Cell division; proteolysis | 3.43 | 0.058 | 0.79 | 0.99
38 | 169831563 | formamidopyrimidine-DNA glycosylase | 9.73 | 31.35 | 7 | 83 | 77 | 95.44 | DNA damage ;DNA repair ;base-excision repair | 0.37 | 0.628 | 4.42 | 0.003
39 | 169831563 |  | 8.7 | 34.35 | 7 | 57 | 77 | 95.44 |  | 0.48 | 0.963 | 5.22 | 0.039
62 | 134037078 | DNA damage checkpoint protein rad24 | 5 | 26.53 | 8 | 33 | 158 | 100 | Protein domain specific binding | 0.3 | 0.005 | 0.97 | 0.993
64 | 134037086 | DNA damage checkpoint protein rad17 | 4.96 | 27.33 | 9 | 17 | 168 | 100 | 14-3-3 family; Protein domain specific binding | 0.38 | 0.136 | 1.63 | 0.13
Oxidative stress response
10 | 112253644 | heat shock protein 70 | 5.1 | 70.14 | 21 | 91 | 492 | 100 | Response to stress | 0.82 | 0.741 | 2.24 | 0.002
11 | 112253644 |  | 4.93 | 28.48 | 1 | 35 | 444 | 97.85 |  | 2.01 | 0.002 | 0.53 | 0.075
14 | 294873483 |  | 7.22 | 34.64 | 1 | 87 | 55 | 99.9 |  | 0.79 | 0.984 | 3.73 | 0.018
18 | 294873483 |  | 7.22 | 34.64 | 2 | 33 | 111 | 100 |  | 2.1 | 0.365 | 3.43 | 0.031
53 | 26987945 | DNA-binding stress protein | 5.2 | 17.85 | 8 | 19 | 172 | 100 |  | 0.34 | 0.626 | 4.88 | 0.002
59 | 118490698 | cytochrome c oxidase subunit, putative | 5.63 | 22.69 | 2 | 74 | 198 | 99.91 | Cellcular iron ion homeostasis; oxidation reduction;response to stress | 0.19 | 0.004 | 0.37 | 0.014
84 | 26991362 | ketol-acid reductoisomerase | 5.48 | 36.58 | 3 | 27 | 103 | 99.95 | Amino-acid biosynthesis; oxidoreductase | 0.39 | 0.302 | 1.41 | 0.583
95 | 26990544 | alcohol dehydrogenase | 5.61 | 35.89 | 6 | 68 | 110 | 99.99 | Oxidation reduction;zinc ion binding | 0.45 | 0.871 | 7.63 | 0.001
96 | 219848105 | RNA binding S1 domain protein | 5.42 | 27 | 1 | 65 | 54 | 100 | similar to cold shock protein, Response to stress | 0.88 | 0.928 | 2.24 | 0.004
20 | 166030338 | luciferin-binding protein | 5.59 | 75.06 | 26 | 95 | 299 | 100 |  | 0.46 | 0.393 | 2.3 | 0.026
22 | 166030338 |  | 5.59 | 75.06 | 28 | 95 | 519 | 100 | Specific substrate-binding protein, clock-controlled repressor | 2.89 | 0.003 | 1.25 | 0.832
23 | 166030338 |  | 5.59 | 75.06 | 21 | 90 | 108 | 99.98 |  | 0.46 | 0.056 | 0.67 | 0.578
24 | 166030338 |  | 5.51 | 29.08 | 1 | 55 | 460 | 95.04 |  | 1.34 | 0.919 | 6.22 | <0.001
25 | 166030338 |  | 5.59 | 75.06 | 12 | 92 | 107 | 99.98 |  | 1.02 | 1 | 2.03 | 0.013
26 | 166030338 |  | 5.59 | 75.06 | 14 | 97 | 165 | 100 |  | 1.36 | 0.326 | 2 | 0.009
27 | 166030338 |  | 5.59 | 75.06 | 13 | 97 | 96 | 99.75 |  | 0.62 | 0.097 | 0.2 | 0.004
28 | 166030338 |  | 5.59 | 75.06 | 16 | 99 | 330 | 100 |  | 0.39 | 0.008 | 0.82 | 0.453
29 | 166030338 |  | 6.03 | 28.81 | 1 | 95 | 640 | 99.14 |  | 0.4 | 0.02 | 0.22 | 0.006
Translation
6 | 301500981 | elongation factor [NAME] | 4.73 | 26.88 | 2 | 99 | 316 | 100 | Protein biosynthesis; translation elongation factor activity | 0.45 | 0.015 | 0.79 | 0.363
91 | 30468209 |  | 9.81 | 27.69 | 2 | 97 | 141 | 100 |  | 2.89 | 0.013 | 2.34 | 0.058
92 | 30468209 |  | 9.81 | 27.69 | 2 | 95 | 141 | 100 |  | 0.69 | 0.084 | 0.49 | 0.011
61 | 26988588 | elongation factor P | 4.73 | 21.35 | 5 | 99 | 140 | 100 | Elongation factor P family; Protein biosynthesis | 0.49 | 0.077 | 1.1 | 0.921
63 | 167032445 |  | 4.77 | 21.38 | 4 | 95 | 127 | 100 |  | 0.49 | 0.027 | 0.89 | 0.809
50 | 167033775 | Hcp1 family type VI secretion system effector | 5.64 | 18.59 | 6 | 29 | 340 | 100 | as an extracellular T6SS translocon | 0.1 | 0.379 | 4.47 | 0.002
Signal transduction
5 | 26991228 | hypothetical protein PP_4544 | 4.87 | 45.27 | 14 | 99 | 437 | 100 | Signal peptide; chain | 0.69 | 0.22 | 2.92 | <0.001
Transport
2 | 41055863 | nuclear pore complex protein Nup155 | 5.77 | 26.56 | 1 | 99 | 196 | 95.2 | Nucleocytoplasmic transport | 2.07 | 0.02 | 2.32 | 0.007
65 | 41055863 |  | 5.77 | 26.56 | 1 | 97 | 196 | 95.58 |  | 2.1 | 0.083 | 0.97 | 1
97 | 41055863 |  | 5.77 | 26.56 | 3 | 94 | 196 | 97.13 |  | 0.44 | 0.014 | 1.2 | 0.405
4 | 26992088 | ATP synthase F1, beta subunit | 4.88 | 49.39 | 12 | 89 | 445 | 100 | ATP synthesis coupled proton transport | 0.67 | 0.933 | 2.59 | 0.06
48 | 222053555 |  | 4.89 | 51 | 7 | 95 | 355 | 100 |  | 0.89 | 0.963 | 2.01 | 0.022
46 | 156350099 | hypothetical protein NEMVEDRAFT_v1g142315 | 5.77 | 26.56 | 1 | 85 | 310 | 95.57 | Nucleocytoplasmic transport | 0.39 | 0.007 | 2.02 | <0.001
81 | 156350099 |  | 5.77 | 26.56 | 1 | 95 | 310 | 95.39 |  | 0.82 | 0.984 | 2.04 | 0.195
58 | 294929773 | UDP-N-acetylglucosamine–dolichyl-phosphate | 8.17 | 28.52 | 2 | 43 | 416 | 100 | transferase | 1.12 | 0.97 | 2.37 | 0.012
66 | 26988814 | [NAME] family protein | 4.72 | 37.22 | 2 | 93 | 700 | 100 | Membrane; porin activity | 0.49 | 0.075 | 1.32 | 0.294
87 | 26987153 | polyamine ABC transporter, periplasmic polyamine-binding protein | 5.62 | 37.89 | 4 | 65 | 118 | 99.99 | Transporter activity | 1.15 | 0.926 | 2.13 | 0.005
Protein biosynthesis
85 | 134037068 | [NAME] | 5.26 | 41.95 | 8 | 57 | 187 | 100 | Protein and  ATP binding | 0.9 | 0.998 | 3.79 | 0.013
98 | 86355884 | formyltetrahydrofolate deformylase | 6.26 | 32.96 | 4 | 41 | 156 | 100 | de novo' IMP biosynthetic process | 0.82 | 0.929 | 2.44 | 0.014
Uncharacterized proteins
13 | 294955276 | hypothetical protein Pmar_PMAR010017 | 11.15 | 27.93 | 1 | 99 | 485 | 95.07 | uncharacterized | 0.78 | 0.336 | 2.1 | <0.001
35 | 294950255 | hypothetical protein Pmar_PMAR005244 | 9.18 | 25.3 | 1 | 66 | 416 | 98.9 |  | 0.5 | 0.002 | 0.05 | <0.001
57 | 294950255 |  | 6.03 | 27.81 | 1 | 47 | 416 | 97.84 |  | 0.64 | 0.181 | 0.48 | 0.053
90 | 294950255 |  | 6.03 | 27.81 | 1 | 68 | 416 | 97.71 |  | 1.57 | 0.162 | 0.48 | 0.209
Unknown proteins
21 |  |  | 8.89 | 20.88 | 2 |  | 165 | 100 |  | 0.92 | 0.977 | 2.05 | 0.01
44 |  |  | 10.25 | 24.23 | 1 |  | 38 | 96.9 |  | 0.44 | 0.098 | 1.91 | 0.014
Unidentified proteins
30 |  |  |  |  |  |  |  |  |  | 0.18 | <0.001 | 0.36 | 0.001
43 |  |  |  |  |  |  |  |  |  | 0.48 | 0.104 | 0.82 | 0.753
51 |  |  |  |  |  |  |  |  |  | 1.34 | 0.73 | 2.63 | 0.011
80 |  |  |  |  |  |  |  |  |  | 0.5 | 0.022 | 0.72 | 0.18
86 |  |  |  |  |  |  |  |  |  | 0.34 | 0.059 | 0.72 | 0.525
99 |  |  |  |  |  |  |  |  |  | 0.46 | 0.145 | 1.1 | 0.959
100 |  |  |  |  |  |  |  |  |  | 0.35 | 0.009 | 0.78 | 0.368
101 |  |  |  |  |  |  |  |  |  | 0.32 | 0.094 | 1.35 | 0.478
102 |  |  |  |  |  |  |  |  |  | 0.36 | 0.862 | 7.44 | 0.001
a  Spot ID represents the protein spot number on the 2-D gels.
b  Accession numbers according to the NCBI database.
c  Spot abundance is expressed as the average optical density of up-regulated or down-regulated proteins at T24, T28 and T34.