Spreadsheet: 239d69d5-acdd-49c5-b96c-233ffb6164be
Sheet: Sheet1

ADDITIONAL FILE 1 | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13 | Unnamed: 14 | Unnamed: 15 | Unnamed: 16 | Unnamed: 17
Clinical and biological characteristics of 90 NT specimens included in the study.
INSS= International Neuroblastoma Staging System; Diagnosis: NB = neuroblastoma, GNB = ganglioneuroblastoma, GN = ganglioneuroma.
Disease status: A = alive, D = dead; EFS= Event free survival; qRT-PCR= Quantitative real-time PCR; IHC= immunohistochemical analysis; [NAME] not available data.
Immunohistochemical analysis of CHD5 expression in NTs.
Results are displayed as percentage of CHD5 immunopositive cells present in each tumor specimen.
Staining intensity: 0 = negative; 1 = weak staining; 2 = strong staining, 3 = very intense staining. Proportion of positive cells values (<25% = 1; 25-75 = 2; >75% = 3).
Intensity and positive cell values were multiplied together to provide a single score for each case.
 |  |  | PATIENT AND TUMOR CHARACTERISTICS |  |  |  |  |  |  |  | IMMUNOHISTOCHEMICAL ANALYSIS OF CHD5
Tumor ID# |  | Diagnosis | INSS stage | Age | MYCN | 1p | Follow-up | Status | EFS | Event |  | Neuroblastic cell |  | [NAME] cell |  | Schwannian cell
 | Specimen type |  | 0=stages 1,2,3;         1= stage 4; 2=stage 4s | 0<12m; 1>12m | 0= Non- Amplified;          1= Amplified | 0=normal;  1=LOH | months | D = Dead;        A = Alive | months | 0= no event;     1= event | % pos. Cells | Intensity | % pos. Cells | Intensity | % pos. | Intensity | IHC Score
NT1 | Dx | NB | 1 | 0 | 0 | 0 | 45.73 | D | 39.1 | 1 | < 25 | 0 |  |  |  |  | 0
NT2 | Dx | NB | 1 | 1 | 0 | 1 | 112.7 | A | 112.7 | 0 | < 25 | 0 |  |  |  |  | 0
NT3 | Dx | NB | 1 | 1 | 0 | 0 | 55.2 | A | 53.7 | 1 | 25-75 | 1 |  |  |  |  | 2
NT4 | Dx | NB | 1 | 1 | 0 | 0 | 33.9 | A | 2.3 | 1 | < 25 | 0 |  |  |  |  | 0
NT5 | Dx | NB | 1 | 1 | 0 | 1 | 70.66 | A | 18.7 | 1 | 25-75 | 1 |  |  |  |  | 2
NT6 | Dx | NB | 1 | 1 | 1 | 1 | 47.87 | A | 47.87 | 0 | <25 | 1 |  |  |  |  | 1
NT7 | Dx | NB | 1 | 1 | 0 | 0 | 53 | D | 1.77 | 1 | <25 | 1 |  |  |  |  | 1
NT8 | Dx | NB | 1 | 1 | 0 | 0 | 49.17 | A | 45 | 1 | <25 | 1 |  |  |  |  | 1
NT9 | Dx | NB | 1 | 1 | 0 | n.a | 27.3 | A | 27.3 | 0 | <25 | 1 |  |  |  |  | 1
NT10 | Dx | NB | 1 | 1 | 0 | 0 | 94.4 | A | 19.57 | 0 | 25-75 | 1 |  |  |  |  | 2
NT11 | Dx | NB | 1 | 1 | 1 | n.a | 28.63 | A | 28.63 | 0 | >75 | 2 |  |  |  |  | 6
NT12 | Dx | NB | 1 | 1 | 0 | 0 | 28.13 | D | 28.13 | 1 | < 25 | 0 |  |  |  |  | 0
NT13 | Dx | NB | 1 | 1 | 1 | 1 | 9.9 | D | 7.2 | 1 | < 25 | 0 |  |  |  |  | 0
NT14 | Dx | NB | 1 | 1 | 0 | 0 | 25.73333333333333 | D | 25.73 | 1 | < 25 | 0 |  |  |  |  | 0
NT15 | Dx | NB | 1 | 1 | 1 | n.a | 34.43 | A | 5 | 1 | <25 | 1 |  |  |  |  | 1
NT16 | Dx | NB | 1 | 1 | 1 | 0 | 8.966666666666667 | D | 8.97 | 1 | < 25 | 0 |  |  |  |  | 0
NT17 | Dx | NB | 1 | 1 | 1 | 1 | 1.7 | D | 1.7 | 1 | <25 | 1 |  |  |  |  | 1
NT18 | Dx | NB | 1 | 1 | 0 | 1 | 172.23 | A | 172.23 | 0 | <25 | 1 |  |  |  |  | 1
NT19 | Dx | NB | 1 | 1 | 0 | 1 | 84.8 | A | 84.8 | 0 | <25 | 1 |  |  |  |  | 1
NT20 | Dx | NB | 1 | 1 | n.a | n.a | 6.13 | D | 6.13 | 1 | <25 | 1 |  |  |  |  | 1
NT21 | Dx | NB | 1 | 1 | n.a | n.a | 23.73 | D | 11 | 1 | <25 | 1 |  |  |  |  | 1
NT22 | Dx | NB | 1 | 1 | n.a | n.a | 11 | D | 11 | 1 | <25 | 1 |  |  |  |  | 1
NT23 | Dx | NB | 1 | 1 | 0 | 1 | 36.93 | D | 26.5 | 1 | <25 | 1 |  |  |  |  | 1
NT24 | Dx | NB | 1 | 1 | 0 | 0 | 40.27 | D | 2.6 | 1 | <25 | 1 |  |  |  |  | 2
NT25 | Dx | NB | 2 | 0 | 0 | 0 | 145.47 | A | 145.47 | 0 | > 75 | 2 |  |  |  |  | 6
NT26 | Dx | NB | 2 | 0 | 0 | 0 | 118.37 | A | 118.37 | 0 | > 75 | 2 |  |  |  |  | 6
NT27 | Dx | NB | 2 | 0 | 0 | 1 | 52.86666666666667 | A | 52.87 | 0 | > 75 | 3 |  |  |  |  | 9
NT28 | Dx | NB | 2 | 0 | 0 | 0 | 34.93333333333334 | A | 34.93 | 0 | > 75 | 3 |  |  |  |  | 9
NT29 | Dx | NB | 2 | 0 | 0 | 0 | 200.37 | A | 39.27 | 1 | >75 | 1 |  |  |  |  | 3
NT30 | Dx | NB | 2 | 0 | 0 | 0 | 121.13 | A | 39.86 | 1 | >75 | 3 |  |  |  |  | 9
NT31 | Dx | NB | 2 | 0 | 0 | 0 | 105.7 | A | 105.7 | 0 | >75 | 3 |  |  |  |  | 9
NT32 | Dx | NB | 2 | 0 | 0 | 1 | 192.2950819672131 | A | 192.3 | 0 | > 75 | 2 |  |  |  |  | 6
NT33 | Dx | NB | 0 | 1 | 0 | 0 | 221.9333333333333 | A | 221.93 | 0 | <25 | 3 |  |  |  |  | 3
NT34 | Dx | NB | 0 | 0 | 0 | 0 | 209.1147540983607 | A | 209.11 | 0 | 25-75 | 2 |  |  |  |  | 4
NT35 | Dx | NB | 0 | 0 | 0 | 0 | 136.83 | A | 136.83 | 0 | 25-75 | 2 |  |  |  |  | 4
NT36 | Dx | NB | 0 | 0 | 0 | 0 | 121.37 | A | 121.37 | 0 | > 75 | 3 |  |  |  |  | 9
NT37 | Dx | NB | 0 | 0 | 0 | 0 | 115.0491803278689 | A | 115.05 | 0 | 25-75 | 3 |  |  |  |  | 6
NT38 | Dx | NB | 0 | 0 | 0 | 0 | 112.5 | A | 112.5 | 0 | >75 | 2 |  |  |  |  | 6
NT39 | Dx | NB | 0 | 0 | 0 | 0 | 108.0666666666667 | A | 108.07 | 0 | <25 | 1 |  |  |  |  | 1
NT40 | Dx | NB | 0 | 0 | 0 | n.a | 64.3 | A | 64.3 | 0 | >75 | 2 |  |  |  |  | 6
NT41 | Dx | NB | 0 | 0 | 0 | 0 | 33.9 | A | 33.9 | 0 | 25-75 | 3 |  |  |  |  | 6
NT42 | Dx | NB | 0 | 0 | 0 | 0 | 32.86666666666667 | A | 32.87 | 0 | 25-75 | 3 |  |  |  |  | 6
NT43 | Dx | NB | 0 | 0 | 0 | 1 | 192.27 | A | 21.7 | 1 | >75 | 2 |  |  |  |  | 6
NT44 | Dx | NB | 0 | 0 | 0 | 0 | 154.5221052631579 | A | 8 | 1 | <25 | 1 |  |  |  |  | 1
NT45 | Dx | NB | 0 | 1 | 0 | 0 | 129.3114754098361 | A | 129.31 | 0 | 25-75 | 3 |  |  |  |  | 6
NT46 | Dx | NB | 0 | 1 | 0 | 0 | 26.83333333333333 | A | 26.83 | 0 | >75 | 3 |  |  |  |  | 9
NT47 | Dx | NB | 0 | 1 | 0 | 0 | 25.8 | A | 25.8 | 0 | 25-75 | 2 |  |  |  |  | 4
NT48 | Dx | NB | 0 | 1 | 0 | 0 | 148.87 | A | 148.87 | 0 | >75 | 1 |  |  |  |  | 3
NT49 | Dx | NB | 0 | 1 | 0 | 0 | 117.2 | A | 6.13 | 0 | >75 | 2 |  |  |  |  | 6
[NAME] | Dx | NB | 0 | 1 | 0 | n.a | 35.03 | A | 35.03 | 0 | 25-75 | 2 |  |  |  |  | 4
NT51 | Dx | NB | 0 | 1 | 0 | 0 | 81.43 | A | 81.43 | 0 | >75 | 2 |  |  |  |  | 6
NT52 | Dx | NB | 0 | 1 | 0 | 1 | 121.3 | A | 121.3 | 0 | >75 | 2 |  |  |  |  | 6
NT53 | Dx | NB | 0 | 1 | 0 | 0 | 46.97 | A | 46.97 | 0 | >75 | 2 |  |  |  |  | 6
NT54 | Dx | NB | 0 | 1 | 0 | 1 | 41.37 | D | 9.53 | 1 | <25 | 1 |  |  |  |  | 1
NT55 | Dx | NB | 0 | 1 | 0 | 0 | 81.53 | D | 7.7 | 1 | <25 | 1 |  |  |  |  | 1
NT56 | Dx | NB | 0 | 1 | 1 | 1 | 22.33333333333333 | D | 13.5 | 1 | 25-75 | 1 |  |  |  |  | 2
NT57 | Dx | NB | 1 | 1 | 1 | 1 | 25.97 | D | 25.97 | 1 | <25 | 1 |  |  |  |  | 1
NT58 | Dx | NB | 0 | 0 | 0 | 0 | 162.9 | A | 162.9 | 0 | >75 | 3 |  |  |  |  | 9
NT59 | Dx | NB | 0 | 1 | 0 | 0 | 145.5 | A | 145.5 | 0 | <25 | 1 |  |  |  |  | 1
NT60 | Dx | NB | 0 | 1 | 1 | 1 | 6.87 | D | 6.87 | 1 | <25 | 1 |  |  |  |  | 1
NT61 | Dx | NB | 0 | 1 | n.a | n.a | 13.23 | D | 13.23 | 1 | <25 | 1 |  |  |  |  | 1
NT62 | Dx | NB | 0 | 1 | n.a | n.a | 14.1 | D | 14.1 | 1 | <25 | 1 |  |  |  |  | 1
NT63 | Dx | NB | 0 | 1 | 0 | 0 | 67.2 | D | 8.87 | 1 | >75 | 2 |  |  |  |  | 6
[NAME] | Dx | GNB |  | 0 | 0 | n.d | 10.96666666666667 | A | 10.96666666666667 | 0 |  |  | > 75 | 2 | <25 | 0
NT65 | Dx | GNB |  | 1 | 0 | n.d | 210.6885245901639 | A | 210.6885245901639 | 0 |  |  | > 75 | 2 | <25 | 0
NT66 | Dx | GNB |  | 1 | 0 | n.d | 112.9 | A | 112.9 | 0 |  |  | > 75 | 1 | <25 | 0
NT67 | Dx | GNB |  | 1 | 0 | n.d | 84.2 | A | 84.2 | 0 |  |  | > 75 | 2 | <25 | 0
NT68 | Dx | GNB |  | 1 | 0 | n.d | 71.2 | A | 71.2 | 0 |  |  | > 75 | 2 | <25 | 0
NT69 | Dx | GNB |  | 1 | 0 | n.d | 71.2 | A | 71.2 | 0 |  |  | > 75 | 1 | <25 | 0
NT70 | Dx | GNB |  | 1 | 0 | n.d | 66.42622950819673 | A | 66.42622950819673 | 0 |  |  | > 75 | 2 | <25 | 0
NT71 | Dx | GNB |  | 1 | 0 | n.d | 2.524590163934426 | A | 2.524590163934426 | 0 |  |  | >75 | 2 | <25 | 0
NT72 | Dx | GNB |  | 1 | 0 | n.d | 30.4 | D | 20.3 | 1 |  |  | > 75 | 2 | <25 | 0
NT73 | Dx | GNB |  | 1 | 0 | n.d | 6.7 | D | 6.7 | 1 |  |  | > 75 | 1 | <25 | 0
NT74 | Dx | GNB |  | 1 | 0 | n.d | 11.96666666666667 | A | 11.96666666666667 | 1 |  |  | > 75 | 2 | <25 | 0
NT75 | Dx | GNB |  | 1 | 0 | n.d | n.a | n.a | n.a | n.a |  |  | >75 | 3 | <25 | 0
NT76 | Dx | GNB |  | 1 | 0 | n.d | n.a | n.a | n.a | n.a |  |  | >75 | 3 | <25 | 0
NT77 | REL | GNB |  | 1 | 0 | n.d | n.a | n.a | n.a | n.a |  |  | >75 | 2 | <25 | 0
NT78 | Dx | GN |  | 1 | 0 | n.d | 34.1 | A | 34.1 | 0 |  |  | > 75 | 2 | <25 | 0
NT79 | Dx | GN |  | 1 | 0 | n.d | 146.4 | A | 146.4 | 0 |  |  | > 75 | 1 | <25 | 0
NT80 | Dx | GN |  | 1 | 0 | n.d | 96.83 | A | 96.83 | 0 |  |  | > 75 | 3 | <25 | 0
NT81 | Dx | GN |  | 1 | 0 | n.d | 84.3 | A | 84.3 | 0 |  |  | > 75 | 1 | <25 | 0
NT82 | Dx | GN |  | 1 | 0 | n.d | 84.3 | A | 84.3 | 0 |  |  | > 75 | 1 | <25 | 0
NT83 | Dx | GN |  | 1 | 0 | n.d | 75.53333333333333 | A | 75.53333333333333 | 0 |  |  | > 75 | 3 | <25 | 0
NT84 | Dx | GN |  | 1 | 0 | n.d | 66.36666666666667 | A | 66.36666666666667 | 0 |  |  | > 75 | 1 | <25 | 0
NT85 | Dx | GN |  | 1 | 0 | n.d | 55.2 | A | 55.2 | 0 |  |  | > 75 | 3 | <25 | 0
NT86 | Dx | GN |  | 1 | 0 | n.d | 53.87 | A | 53.87 | 0 |  |  | > 75 | 2 | <25 | 0
NT87 | Dx | GN |  | 1 | 0 | n.d | 1319.4 | A | 1319.4 | 0 |  |  | > 75 | 3 | <25 | 0
NT88 | Dx | GN |  | 0 | 0 | n.d | n.a | n.a | n.a | n.a |  |  | >75 | 2 | <25 | 0
NT89 | Dx | GN |  | 1 | 0 | n.d | n.a | n.a | n.a | n.a |  |  | >75 | 3 | <25 | 0
NT90 | Dx | GN |  | 1 | 0 | n.d | n.a | n.a | n.a | n.a |  |  | >75 | 2 | <25 | 0