Spreadsheet: 47a48b89-675f-4100-9a52-67f6932cb229
Sheet: LIBRARY DESIGN

Sequence # | 12-mer (5' to 3') | core | binding to Her1 |  |  | binding to Her7:Hes6 heterodimer |  |  | promoter of origin | distance from ATG | tested in Y1H | fragment | Interaction with Her1 in Y1H
 |  |  | [NAME] affinity | standard error | R2 of fit | [NAME] affinity | standard error | R2 of fit
1 | GCCCACGGGCCC | CACGGG | 0.1442976104756832 | 0.03133015158369224 | 0.5115754 | 0.02792606747000886 | 0.001712640025764204 | 0.8974 | [NAME] | -7531 | N | n.a. | n.a.
2 | TGGCACGTGTCC | CACGTG | 1 | 0.03212932750518172 | 0.9536019 | 1 | 0.05121459434797778 | 0.891 | [NAME] | -6129 | N | n.a. | n.a.
3 | AGCCTCGTGTAA | [NAME] | 0.03627877159124064 | 0.006270893058057563 | 0.3029703 | 0.05162502885054078 | 0.002440273207911758 | 0.9201 | [NAME] | -6062 | N | n.a. | n.a.
4 | CTGCACGTGCAT | CACGTG | 0.03914082944983212 | 0.002322947588571041 | 0.8724591 | 0.1580374117710207 | 0.002225355733877245 | 0.9924 | [NAME] | -3176 | N | n.a. | n.a.
5 | CGACACGCGGTG | CACGCG | 0.04075308248726096 | 0.002246365212394726 | 0.8300443 | 0.08486014868092644 | 0.001744796972706046 | 0.9842 | [NAME] | -3137 | N | n.a. | n.a.
6 | ATCCACGTGTCC | CACGTG | 0.3028484957176648 | 0.01454247849909928 | 0.9116973 | 0.2559316175089236 | 0.003860881887227933 | 0.9915 | [NAME] | -2931 | Y | -2578 to -3063 | Y
7 | CATCACGTGAAA | CACGTG | 0.01799212503580138 | 0.00635920757838159 | -0.7324432 | 0.03437253428517753 | 0.003479540001610262 | 0.4531 | [NAME] | -2351 | Y | -1650 to -2598 | N
8 | CAGCGCGTGCCT | CACGCG | 0.2270668212036935 | 0.01067816058110205 | 0.9010242 | 0.463624164676203 | 0.004259779930758702 | 0.9967 | [NAME] | -1437 | Y | -1094 to -1672 | Y
9 | TTGCTCGTGCTT | [NAME] | 0.02350633985476018 | 0.001917668882113767 | 0.632432 | 0.1091599796033386 | 0.001951018491183811 | 0.9842 | [NAME] | -1394 | Y | -1094 to -1672 | Y
10 | AGACACGCGCTA | CACGCG | 0.1180267620299313 | 0.003851763122932777 | 0.9483578 | 0.2749030890206919 | 0.002667628888113577 | 0.9964 | [NAME] | -1359 | Y | -1094 to -1672 | Y
11 | TGACGCGTGTGA | CACGCG | 0.1180318478327788 | 0.005953663874828577 | 0.8394681 | 0.386804755642629 | 0.005299561471780146 | 0.9931 | [NAME] | -803 | Y | -719 to -1114 | N
12 | TGTCGCGTGCCA | CACGCG | 0.5967167127511784 | 0.02459527270164093 | 0.8903387 | 0.531492552534822 | 0.009638941520624783 | 0.9886 | [NAME] | -672 | Y | 0 to -736 | N
13 | TATCTCGTGCTG | [NAME] | 0.02895690183597483 | 0.00109976114571539 | 0.945219 | 0.07581879176618984 | 0.008242473363570488 | 0.6755 | [NAME] | -468 | Y | 0 to -736 | N
14 | AAACACGAGCCA | [NAME] | 0.05618952021249734 | 0.002472044590891774 | 0.8883146 | 0.2084552212769384 | 0.003060625855452081 | 0.9918 | [NAME] | 2 | N | n.a. | n.a.
15 | CTGCTCGTGCTT | [NAME] | 0.01974701253695015 | 0.001161657150861865 | 0.7009055 | 0.103264431979818 | 0.003146876895413435 | 0.948 | [NAME] | 203 | N | n.a. | n.a.
16 | CAGCGCGTGTGC | CACGCG | 0.05696598847039572 | 0.001497622610230315 | 0.9540907 | 0.1788387858618931 | 0.00224771637905585 | 0.994 | [NAME] | 1083 | N | n.a. | n.a.
17 | TGCCGCGTGCAC | CACGCG | 0.03299864289366122 | 0.002144330623581886 | 0.4913351 | 0.1625289176350608 | 0.001879307587021282 | 0.994 | [NAME] | 1373 | N | n.a. | n.a.
18 | GTACACGTGGCG | CACGTG | 0.2372335195450972 | 0.007931380920387556 | 0.9104066 | 0.2747992807493089 | 0.002805774938944205 | 0.9957 | [NAME] | 1508 | N | n.a. | n.a.
19 | TTCCACGGGTCT | CACGGG | 0.04105906044699746 | 0.002122268946177037 | 0.9141594 | 0.05873766136174553 | 0.002065654169238615 | 0.9379 | [NAME] | 3492 | N | n.a. | n.a.
20 | TTGCACGTGTAC | CACGTG | 0.05558787865809754 | 0.002102399517473302 | 0.9139993 | 0.1784968197310861 | 0.002368547274630311 | 0.9932 | her7 | 1 | N | n.a. | n.a.
21 | AGGCGCGTGGTG | CACGCG | 0.07702145048881703 | 0.005061881729208659 | 0.4356447 | 0.1566663803977349 | 0.004027340651082902 | 0.9644 | her7 | -160 | Y | 0 to -669 | Y
22 | GGGCACGTGCGC | CACGTG | 0.4060471088103058 | 0.008988621185313272 | 0.9576017 | 0.5418110088296074 | 0.01660301655886852 | 0.9661 | her7 | -591 | Y | 0 to -669 | Y
23 | GCCCACGCGCCG | CACGCG | 0.1188437025715425 | 0.007291247868647097 | 0.602333 | 0.1676653337269531 | 0.002506061565712139 | 0.9891 | her7 | -640 | Y | 0 to 669 | Y
24 | CGGCGCGTGCCA | CACGCG | 0.7303811586172684 | 0.03161381446812763 | 0.7903684 | 0.9590333056010305 | 0.03564233380746625 | 0.8784 | her7 | -1265 | Y | -660 to -1667 | N
24 | CGGCGCGTGCCA | CACGCG | 0.7303811586172684 | 0.03161381446812763 | 0.7903684 | 0.9590333056010305 | 0.03564233380746625 | 0.8784 | her7 | -1690 | Y | -1647 to -2491 | Y
25 | GGTCGCGTGTGC | CACGCG | 0.1806875113203726 | 0.01145612781247128 | 0.6859424 | 0.1822917796087062 | 0.003165975148278361 | 0.9853 | her7 | -1794 | Y | -1647 to -2491 | Y
26 | GGGCGCGTGCCG | CACGCG | 0.5761205495879161 | 0.01013044638180817 | 0.9771916 | 0.7475945358417648 | 0.02104701967204316 | 0.9632 | her7 | -1829 | Y | -1647 to -2491 | Y
27 | TGCCGCGTGCCG | CACGCG | 0.2157279791428549 | 0.01040028837394392 | 0.8743106 | 0.3689709347575212 | 0.003226359464319261 | 0.9971 | her7 | -1836 | Y | -1647 to -2491 | Y
28 | GCGCTCGTGCGC | [NAME] | 0.02913239772405861 | 0.005076800084228034 | -0.1048244 | 0.08606226349265989 | 0.00202288505407799 | 0.9713 | her7 | -1868 | Y | -1647 to -2491 | Y
29 | GTTCGCGTGTGC | CACGCG | 0.04524025957223936 | 0.001232592501385212 | 0.9433401 | 0.1004165749711494 | 0.002058457905048174 | 0.9777 | her7 | -1885 | Y | -1647 to -2491 | Y
30 | CAGCACGTGTCT | CACGTG | 0.4564255549993888 | 0.009803670165163678 | 0.9809582 | 0.6788126996054856 | 0.01399101473390408 | 0.984 | her7 | -2055 | Y | -1647 to -2491 | Y
31 | AGCCACGTGGCT | CACGTG | 0.570228067029097 | 0.01928570345129719 | 0.9367141 | 0.3466094844475457 | 0.00812751670647594 | 0.9745 | her7 | -2159 | Y | -1647 to -2491 | Y
32 | CCGCGCGTGCCA | CACGCG | 0.2789617288874494 | 0.01294774025288039 | 0.8439367 | 0.5159491693728027 | 0.01791634148305199 | 0.9573 | her7 | -3063 | Y | -2471 to -3031 | Y
33 | GTTCTCGTGTTT | [NAME] | 0.01708339021402307 | 0.001399273890112753 | 0.6452129 | 0.02649427014841255 | 0.001599076782695043 | 0.8884 | her7 | -3332 | Y | -3010 to -3513 | Y
34 | TCCCACGGGAAT | CACGGG | 0.01025193461263581 | 0.0008300423727679348 | -0.01001227 | 0.01031483320361772 | 0.001083703067550522 | -0.1648 | her7 | -4153 | Y | -3490 to -4185 | N
35 | AAGCACGTGATC | CACGTG | 0.04231193459479089 | 0.001269561826856073 | 0.9416667 | 0.09117323743324118 | 0.00179661147043826 | 0.9824 | her1/her7 | -6720 from her7 -5584 from her1 | N | n.a. | n.a.
36 | CTCCTCGTGTTG | [NAME] | 0.01769034955525992 | 0.0005566485273031771 | 0.9596061 | 0.06046762030004563 | 0.002761111618045678 | 0.9316 | her1/her7 | -7812 from her7 -4492 from her1 | N | n.a. | n.a.
37 | CAACACGGGGTG | CACGGG | 0.02602022543490306 | 0.009778999560122665 | -0.5231291 | 0.0311086497946915 | 0.003203616650116744 | 0.3306 | her1 | -3567 | N | n.a. | n.a.
38 | CGACACGTGCTC | CACGTG | 0.4161092359071957 | 0.009329646572391942 | 0.9607186 | 0.6376527736775718 | 0.02955216982904377 | 0.9077 | her1 | -3157 | Y | -3126 to -3220 | Y
39 | CTCCACGTGCCC | CACGTG | 0.2692985250279496 | 0.006645333062090514 | 0.9560956 | 0.2967451759212045 | 0.009261071898231395 | 0.9643 | her1 | -3072 | Y | -3034 to -3128 | Y
40 | [NAME] | CACGGG | 0.02091495377719009 | 0.002568887199569581 | 0.4623883 | 0.02066892998040847 | 0.001684029414132739 | 0.7786 | her1 | -2334 | Y | -1972 to -2685 | Y
41 | GTTCACGTGTGC | CACGTG | 0.03413966506865388 | 0.001384890882761537 | 0.8630677 | 0.1020982797026381 | 0.004861791685676713 | 0.9118 | her1 | -2171 | Y | -1972 to -2686 | Y
42 | TTCCACGAGAAA | [NAME] | 0.01285007499328585 | 0.0006724879479856207 | 0.8252285 | 0.02856498752046375 | 0.001268632081801347 | 0.9241 | her1 | -1152 | Y |  | N
43 | ATACACAGTGCGC | CACNGTG | 0.01280704196322697 | 0.0004249606742525432 | 0.9110474 | 0.02083871071629854 | 0.00209984809854808 | 0.5723 | her1 | -2493 | Y | -1972 to -2685 | Y
44 | TCACACCTGTGCAG | CACNNGTG | 0.009426223291727896 | 0.0002862718120715546 | 0.9136933 | 0.02002931751697485 | 0.001332758648452806 | 0.7427 | her7 | -1851 | Y | -1647 to -2491 | Y
45 | GCTCAGGTGCGT | CACCTG | 0.00829409234925725 | 0.0006072544070266166 | 0.1890715 | 0.01343025683690722 | 0.0009292203644561338 | 0.7889 | her1 | -3089 | Y | -3034 to -3128 | Y
46 | CTCCACAAACCC | CACAAA | 0.01054193244447776 | 0.0006699720816191054 | -0.2248723 | 0.01310490861758944 | 0.001078571696948552 | 0.7209 | control | n.a. | n.a. |  | n.a.
47 | CTCAAAAAACCC | AAAAAA | 0.01045005786216047 | 0.0005394746633332352 | 0.6466395 | 0.01372934167091597 | 0.001123497490673895 | 0.5137 | control | n.a. | n.a. |  | n.a.