Spreadsheet: 9656c155-f310-47f5-9371-44846b2f45cb
Sheet: Sheet1

Supplemental Table S4. Complete listing of Sporozoite Conserved Orthologous Transcripts (SCOT) genes and previously annotated conserved genes. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13 | Unnamed: 14 | Unnamed: 15
A) Sporozoite conserved orthologous transcript (SCOT)  genes were identified as those genes with greater than 2 fold upregulation in sporozoites relative to average asexual gene expression in all species.B) Genes upregulated in P. vivax sporozoites (UVS) were identified as those P. vivax genes upregulated greater than 4 fold and expression level above 600 units in sporozoites, with P. falciparum orthologs upregulated less than 2 fold. C) Genes downregulated in P. vivax sporozoites (DVS) were those genes with expression greater than 600 units and fold change greater than 4-fold in P. falciparum sporozoites relative to average asexual expression, and P. vivax orthologs with expression less than 600 units and fold change less than 2-fold in P. vivax sporozoites relative to average asexual expression.D) P. vivax specific genes without P. falciparum or P. yoelii orthologs that are highly expressed in sporozoites. Exp is the average gene expression for the sporozoite hybridizations. Rank is the ranking of each gene according to the average sporozoite gene expression for all genes. Fold change is equal to the average sporozoite expression divided by the average expression of all asexual samples. P. falciparum salivary gland sporozoite and P. yoelii midgut and salivary gland sporozoite gene expression values were taken from previously published data (LeRoch, et [NAME]. 2003, and [NAME], et [NAME]. 2008). * PF11_0545 was originally annotated on the opposite strand, so previous gene expression data is not accurate. ND = Not Determined
A)
Alias | Description | P. vivax Gene | Exp | Rank | Fold Change Spz/Asex2 | Fold Change Spz/Avg Asexuals | P. falciparum Gene | Exp | Rank | Fold change | P. yoelii [NAME] | Midgut Exp | [NAME] | Rank | Fold Change
[NAME] | hypothetical protein, conserved in Plasmodium species | Pv_PKH_141170 | 17726 | 1 | 18.2 | 9.3 | PF_PKH_141170 | ND | ND | ND | PY_PKH_141170 |  | ND | ND | ND
UIS41 | UIS4, ETRAMP10.3 | Pv001715 | 16095 | 2 | 329 | 216.2 | PF10_0164 | 10763.7 | 3 | 8.875500636102343 | PY00204 | 21021 | 1 | 472.38202247191
SCOT2 | hypothetical protein, conserved | Pv092505 | 13536.05 | 3 | 99.0201170446232 | 52.8256712457071 | PF11_0545* | ND | ND | ND | PY02432 | 4107 | 6846 | 17 | 66.790243902439
CSP1 | circumsporozoite protein precursor, putative | Pv119355 | 13188.85 | 4 | 92.4074268698546 | 106.861529735861 | PFC0210c | 28684.1 | 1 | 93.19070825211175 | PY03168 | 21021 | 18180 | 2 | 303
AMA1 | apical merozoite antigen 1 | Pv092275 | 4855.55 | 10 | 79.3714752758479 | 59.2501525320317 | PF11_0344 | 1513.4 | 132 | 6.250213870615653 | PY01581 | 615 | 284.666666666666 | 1255 | 3.09420289855072
ECP12 | cysteine protease serine-repeat antigen (SERA) | Pv003790 | 4799.4 | 11 | 119.611214953271 | 94.6813967251923 | PFB0325c | 464.8 | 317 | 46.48 | PY02063 | 3102 | 901 | 498 | 11.0552147239263
 | Falstatin | Pv099035 | 4357.85 | 13 | 3.99793582715992 | 7.10870593608795 | PFI0580c | 2592.1 | 93 | 4.286031487321216 | PY03424 | 620 | 2233 | 198 | 7.79406631762652
PvROM13 | rhomboid-like protease 1, putative | Pv091350 | 3501.25 | 15 | 20.940490430622 | 28.2563957711242 | PF11_0150 | 524.8 | 292 | 6.492753623188405 | PY00729 | 789 | 585.333333333333 | 695 | 5.62820512820512
SCOT3 | hypothetical protein, conserved | Pv085040 | 2794 | 23 | 8.7 | 10.6 | PF14_0279 | 50.3 | 1976 | 3.8 | PY01270 | 97 | 136 | 2248 | 4.13
SCOT4 | hypothetical protein, conserved | Pv123360 | 2793.4 | 24 | 13.8184516448182 | 17.2261963492846 | PFL0650c | 65 | 1701 | 3.350515463917526 | PY05921 | 516 | 539.333333333333 | 748 | 5.36650082918739
SPECT4 | sporozoite microneme protein, putative | Pv083025 | 2671 | 29 | 8.5 | 11.9 | MAL13P1.212 | 1528.7 | 131 | 152.87 | PY07307 | ND | ND | ND | ND
D135 | CCCH zinc finger domain D13 protein, putative | Pv089510 | 2281.75 | 35 | 11.8779281624154 | 5.83508081014729 | MAL8P1.70 | 528.3 | 290 | 3.02354672553348 | PY03958 | 3190 | 2985 | 109 | 21.8681318681318
SCOT5 | hypothetical protein, conserved | Pv094795 | 2063.25 | 43 | 4.43900602409638 | 6.58533082250805 | PF10_0112 | 427.1 | 341 | 4.850653038046564 | PY06766 | 818 | 3106.66666666666 | 102 | 41.7002237136465
S146 | S14 P. yoelii Sporozoite-specific gene | Pv084410 | 2037.2 | 46 | 8.93019178082191 | 9.99803690616411 | PFL0370w | 733.6 | 223 | 73.36 | PY04162 | 4023 | 900.333333333333 | 499 | 13.6414141414141
 | mitochondrial ribosomal protein L22/L43 | Pv094720 | 1968.15 | 53 | 7.21528732471817 | 11.9180695167736 | PF10_0097 | 330.5 | 442 | 3.050702182369619 | PY04009 | 254 | 632.333333333333 | 665 | 2.30778588807785
SCOT6 | hypothetical protein, conserved | Pv123035 | 1783.15 | 59 | 4.01497326203208 | 3.38454968207269 | PF08_0072 | 115.7 | 1087 | 7.151434878587198 | PY05423 | 4022 | 1026.66666666666 | 453 | 41.0666666666666
SCOT7 | hypothetical protein, conserved | Pv123155 | 1623.55 | 66 | 17.0855038147855 | 12.0111711178515 | PF08_0088 | 200 | 693 | 20 | PY01796 | 4076 | 422.333333333333 | 910 | 14.5632183908046
 | SPATR-like protein, putative | Pv002900 | 1610.15 | 68 | 4.73086528573527 | 5.88225623789865 | PFB0570w | 1203.7 | 153 | 4.118130055472741 | PY02991 | 2511 | 589.333333333333 | 690 | 2.93200663349917
SCOT8 | hypothetical protein, conserved | Pv095375 | 1335.85 | 83 | 8.58101814678014 | 2.92988112470939 | PFC0750w | 726.5 | 227 | 28.65070422535211 | PY00153 | 310 | 225 | 1499 | 8.8235294117647
SCOT9 | Latency-associated protein, putative | Pv080665 | 1316.25 | 86 | 12.5 | 11.675093134646 | PFE0565w | 75.8 | 1520 | 7.58 | PY00913 | 6421 | 6055 | 25 | 173
S16 | [NAME] yoelii Sporozoite-specific gene, Zinc finger protein | Pv094625 | 1312.75 | 87 | 16.246905940594 | 12.9436994675606 | PF10_0083 | 66.8 | 1663 | 6.68 | PY05712 | 18180 | 3791.66666666666 | 68 | 90.2777777777777
ASP7 | aspartyl protease, putative | Pv088125 | 1212.1 | 95 | 6.16687865683032 | 6.08300712636755 | PF08_0108 | 986.1 | 179 | 4.747713047664901 | PY06692 | 4033 | 2920.33333333333 | 117 | 8.00091324200913
SCOT10 | hypothetical protein, conserved | Pv119825 | 1198.65 | 100 | 3.11236611489776 | 2.07705903758512 | PFC0605w | 66.5 | 1666 | 5.002686727565825 | PY02286 | 199 | 429.666666666666 | 896 | 14.087431693989
SCOT11 | hypothetical protein, conserved | Pv084350 | 1172.65 | 102 | 11.3491410597628 | 5.92786371448791 | PFL0315c | 49.5 | 2005 | 4.95 | PY01844 | 766 | 894 | 504 | 16.2545454545454
 | phospholipid scramblase 1, putative | Pv111580 | 1094.4 | 117 | 8.20543580131209 | 8.81940527036828 | PF10_0220 | 468.9 | 314 | 3.748415462799063 | PY01663 | 619 | 331.666666666666 | 1109 | 5.97597597597597
UIS31 | early transcribed membrane protein (ETRAMP13) | Pv121950 | 1082.15 | 119 | 10.4555555555555 | 7.27740416946873 | PF13_0012 | 112.8 | 1116 | 11.28 | PY03011 | 2854 | 14050 | 3 | 62.0309050772626
SCOT12 | hypothetical protein, conserved | Pv096185 | 1074.7 | 121 | 4.23485370899418 | 2.07719664463257 | MAL7P1.125 | 1396.7 | 141 | 29.15431638586551 | PY02049 | 4528 | 1312.33333333333 | 405 | 21.5136612021857
UIS141 | lysine decarboxylase, putative | Pv000955 | 1049.8 | 124 | 7.38645558487247 | 9.36652391149179 | PFD0285c | 152.8 | 859 | 8.00898539872707 | PY00349 | 80 | 488.333333333333 | 806 | 6.87793427230046
 | 3',5'-cyclic-nucleotide phosphodiesterase, putative | Pv084505 | 1032.4 | 130 | 5.9581589958159 | 6.14523809523809 | PFL0475w | 128.7 | 999 | 6.256249999999999 | PY01829 | 302 | 208 | 1609 | 2.08
 | WD domain, G-beta repeat domain containing protein | Pv092555 | 1016 | 134 | 3.8 | 4.7 | PF11_0399 | 979 | 180 | 9.177715280567833 | Py05176 | 41 | 1861 | 279 | 43.3
SCOT13 | hypothetical protein | Pv116750 | 992.4 | 138 | 3.42709142709142 | 3.30370518326176 | MAL13P1.125 | 524.8 | 291 | 52.48 | PY00521 | 24 | 112.333333333333 | 2590 | 11.2333333333333
SCOT14 | hypothetical protein, conserved | Pv101130 | 983.9 | 139 | 4.3787271918113 | 2.75170600738337 | PFL2145w | 935.7 | 182 | 7.792397834751056 | PY00985 | 10123 | 2838.33333333333 | 127 | 4.86015981735159
SCOT15 | hypothetical protein, conserved | Pv118040 | 811.95 | 176 | 8.30639386189258 | 6.75274451097804 | PF14_0467 | 645 | 243 | 64.5 | PY05966 | 4596 | 7966.66666666666 | 14 | 5.67426400759734
 | erythrocyte binding protein 1 | Pv092975 | 811 | 178 | 5.701230228471 | 5.77758780366175 | PF11_0486 | 343.8 | 423 | 25.82188841201717 | PY05977 | 11745 | 487.666666666666 | 809 | 3.98095238095238
 | nifU, Iron-sulfer cluster assembly protein, putative | Pv117755 | 717.7 | 218 | 5.17447728911319 | 6.65152919369786 | PF14_0518 | 806.1 | 204 | 5.657125670459672 | PY00856 | 2852 | 4699 | 36 | 5.59071980963712
SCOT16 | hypothetical protein, conserved | Pv089965 | 704.2 | 223 | 1.79231356579282 | 2.06358974358974 | PFD0840w | 232 | 621 | 3.346728490468831 | PY04535 | 148 | 151.666666666666 | 2058 | 2.0919540229885
 | hypothetical protein, conserved | Pv091430 | 618 | 260 | 3.6 | 3.2 | PF11_0167 | 186 | 743 | 2.3 | PY02160 | 43 | 177 | 2362 | 3.5
SCOT17 | hypothetical protein, conserved | Pv092720 | 597.75 | 271 | 4.91065927295132 | 3.069004466807 | [NAME] | 357.6 | 402 | 14.32036613272311 | PY05500 | 141 | 1056.33333333333 | 449 | 9.6030303030303
SCOT18 | hypothetical protein, conserved | Pv111520 | 535.15 | 318 | 3.16002361972246 | 2.04466434875635 | PF10_0232 | 1096.7 | 169 | 2.869815517466963 | PY02297 | 826 | 1234.33333333333 | 426 | 3.35416666666666
SCOT19 | hypothetical protein, conserved | Pv000940 | 527.95 | 325 | 2.52305854241338 | 2.27231643281398 | PFD0300w | 65.9 | 1681 | 2.258506731946144 | PY05103 | 603 | 871.666666666666 | 516 | 3.50066934404283
SCOT20 | ubiquitin domain containing protein | Pv092205 | 521.55 | 331 | 1.27253873368305 | 2.00928458604615 | PF11_0329 | 210.2 | 671 | 6.040229885057472 | PY03631 | 258 | 179.333333333333 | 1823 | 4.54008438818565
TRAP/SSP2 | TRAP, Thrombospondin-related anonymous protein, SSP2, sporozoite surface protein 2 | Pv082735 | 496.1 | 358 | 4.88888888888888 | 3.73597409443482 | PF13_0201 | 12475.8 | 2 | 347.6536624203821 | PY03052 | 14031 | 9849.33333333333 | 8 | 121.59670781893
SIAP-18 | SIAP-1, Sporozoite Invasion-Associated Protein -1, [NAME] yoelii Sporozoite specific gene | Pv000815 | 492.35 | 360 | 5.85608088016651 | 3.55667124178285 | PFD0425w | 398.9 | 359 | 39.89 | PY00455 | 8038 | 690 | 631 | 8.41463414634146
SCOT21 | hypothetical protein, conserved | Pv001015 | 489.3 | 367 | 2.33138773079213 | 2.11689884918231 | PFD0215c | 792.1 | 207 | 79.21000000000001 | PY01340 | 2115 | 2891 | 119 | 47.7851239669421
SCOT22 | hypothetical protein, conserved | Pv084815 | 479.1 | 385 | 3.3840720466184 | 2.5385471308218 | PF14_0325 | 368.9 | 390 | 2.735922021507655 | PY03139 | 2586 | 1416.66666666666 | 383 | 8.45771144278607
SCOT23 | sugar transporter, putative | Pv099390 | 472.45 | 395 | 2.64603752450294 | 2.08109417672451 | PFI0955w | 238.6 | 608 | 19.91890280262373 | PY05332 | 2840 | 926 | 483 | 4.70050761421319
PSOP97 | plasmodium secreted ookinete protein 9 ortholog | Pv088910 | 461.9 | 409 | 13.4370909090909 | 10.7468590041879 | PF08_0008 | 259.8 | 552 | 5.468651330626974 | PY07130 | 3271 | 2753.33333333333 | 143 | 19.5966785290628
P52 | p52, 6 cysteine-motif domain family protein | Pv001020 | 459.2 | 413 | 5.50104821802935 | 3.10144535998919 | PFD0215c | 792.1 | 207 | 79.21000000000001 | PY01340 | 2115 | 2891 | 119 | 47.7851239669421
[NAME] | TRSP, Thrombospondin-related sporozoite protein | Pv081560 | 457.4 | 418 | 3.96962464742894 | 2.67516668616212 | PFA0200w | 1433.7 | 136 | 143.37 | PY07092 | 7615 | 8352 | 12 | 273.83606557377
PTRAMP10 | Plasmodium thrombospondin-related apical membrane protein | Pv123575 | 432.35 | 460 | 5.20746763023185 | 3.1643855668594 | PFL0870w | 480.4 | 309 | 5.892929115920441 | PY04732 | 233 | 233.666666666666 | 1463 | 4.58169934640522
 | phospholipase [NAME], putative | Pv123050 | 419.6 | 487 | 3.67586508979413 | 3.98367036931548 | MAL8P1.91 | 261.5 | 549 | 7.218059936908517 | PY02403 | 152 | 738.666666666666 | 595 | 18.4666666666666
SCOT24 | RNA-binding protein of pumilio/[NAME] family, putative | Pv089945 | 411.65 | 512 | 4.23181701362117 | 3.1873790166473 | PFD0825c | 124.8 | 1030 | 12.48 | PY04369 | 2095 | 1948.33333333333 | 257 | 66.045197740113
SCOT25 | hypothetical protein, conserved | Pv086045 | 386.35 | 579 | 5.60536815379035 | 2.60185871102431 | PF14_0074 | 2819.3 | 87 | 281.93 | PY04135 | 9680 | 3032.66666666666 | 105 | 23.7856209150326
[NAME] | FIKK calcium-dependent protein kinase 3, putative | Pv091755 | 374.8 | 608 | 4.04533189422558 | 3.43254876820221 | PF11_0239 | 2172.2 | 103 | 30.64060453400504 | PY04265 | 465 | 155.333333333333 | 2023 | 3.93248945147679
 | ubiquitin-specific protease, putative | Pv001075 | 334.8 | 743 | 4.8329123060267 | 3.90073400908773 | PFD0165w | 103.6 | 1193 | 6.114671163575042 | PY02443 | 156 | 203 | 1648 | 6.88135593220339
SCOT26 | hypothetical protein, conserved | Pv123580 | 324.55 | 771 | 5.02399380804953 | 3.21942267632179 | PFL0875w | 111.8 | 1123 | 11.18 | PY04840 | 4400 | 586 | 694 | 9.76666666666666
SCOT27 | hypothetical protein, conserved | Pv083440 | 275.9 | 1030 | 3.56 | 2.04446091144868 | [NAME] | 476.5 | 310 | 47.65 | PY06979 | 649 | 735 | 600 | 35.8536585365853
 | iron-sulfur subunit of succinate dehydrogenase, putative | Pv123345 | 208.8 | 1591 | 2.06579272817215 | 2.20276400464184 | PFL0630w | 2383.2 | 97 | 19.52071144395039 | PY04921 | 2891 | 4080.66666666666 | 49 | 9.24273310683276
PPL111/ SPECT212 | MAC/Perforin, [NAME] yoelii Sporozoite-specific gene, PPL1, SPECT2 | Pv000810 | 202.95 | 1656 | 3.5511811023622 | 2.10332676961343 | PFD0430c | 4333.1 | 17 | 133.5315870570108 | PY00454 | 755 | 3865.33333333333 | 65 | 63.8898071625344
SCOT28 | hypothetical protein, conserved | Pv094705 | 192.15 | 1799 | 4.6357056694813 | 3.45035015263063 | PF10_0095 | 572.2 | 264 | 2.754935002407318 | PY02531 | 607 | 208 | 1608 | 2.5679012345679
SCOT29 | hypothetical protein, conserved | Pv001000 | 167.6 | 2175 | 3.87514450867052 | 2.57965214714483 | PFD0235c | 118.8 | 1062 | 11.88 | PY01067 | 4132 | 283.333333333333 | 1261 | 15.7407407407407
 | S-adenosylmethionine decarboxylase-ornithine decarboxylase, putative | Pv111070 | 149.9 | 2531 | 2.95660749506903 | 2.60831738298242 | PF10_0322 | 5296.6 | 12 | 2.882088873514791 | PY04754 | 801 | 1419.33333333333 | 382 | 3.18951310861423
SCOT30 | hypothetical protein, conserved | Pv092945 | 144.95 | 2642 | 3.4450386215092 | 2.82278481012658 | [NAME] | 1963.7 | 109 | 196.37 | PY03923 | 705 | 357.666666666666 | 1044 | 12.7738095238095
[NAME] | cysteine repeat modular protein, putative | Pv099005 | 129.9 | 3020 | 2.73186119873817 | 2.00679746639888 | PFI0550w | 76.3 | 1513 | 7.63 | PY00337 | 147 | 185 | 1786 | 2.78195488721804
SCOT32 | hypothetical protein, conserved | Pv118150 | 126.5 | 3119 | 3.06480920654149 | 2.04361873990306 | PF14_0447 | 806 | 205 | 14.47780343854247 | PY06896 | 1658 | 921.666666666666 | 488 | 20.2564102564102
Pfs3813 | Pfs38, putative | Pv097960 | 112.45 | 3535 | 1.78633836378077 | 2.00017787264318 | PFE0395c | 579.2 | 259 | 2.777461894159959 | PY02738 | 543 | 432.666666666666 | 890 | 5.73068432671081
B)
Alias | Description | P. vivax Gene | Exp | Rank | Fold Change Spz/Asex2 | Fold Change Spz/Avg Asexuals | P. falciparum Gene | Exp | Rank | Fold Change | P. yoelii [NAME] | Midgut Spz Exp | [NAME] | Rank | Fold Change
UVS1 | hypothetical protein, conserved | Pv089045 | 5424.15 | 9 | 3.36032338501091 | 5.74166402032391 | PF08_0021 | 20.6 | 2942 | 0.479627473806752 | PY05573 | 37 | 54.3333333333333 | 3851 | 0.498470948012232
DIM1 | DIM1 rRNA dimethylase, putative | Pv100520 | 3383.4 | 17 | 4.77628374801482 | 8.1380637402285 | PFL1520w | 52.8 | 1937 | 0.421388667198723 | PY07357 | 108 | 153.666666666666 | 2044 | 0.51565995525727
S116 | S11 P. yoelii conserved hypothetical protein1 | Pv086200 | 3055.7 | 20 | 41.560013600816 | 21.5250774866159 | [NAME] | 9.5 | 3486 | 0.95 | PY06764 | 438 | 1811.66666666666 | 295 | 51.0328638497652
DHFR-TS | bifunctional dihydrofolate reductase-thymidylate synthase 1, putative | Pv089950 | 2729.1 | 25 | 7.0419300735389 | 10.382727791516 | PFD0830w | 10.8 | 3404 | 0.9225137278828555 | PY04370 | 2073 | 2939.66666666666 | 115 | 13.0651851851851
UVS2 | protein tyrosine phosphatase, putative | Pv091305 | 2712.95 | 26 | 5.61861861861861 | 4.12603418907409 | PF11_0139 | 1572.6 | 128 | 1.354538630966297 | PY05564 | 4062 | 5308.33333333333 | 28 | 2.03501373714139
UVS3 | hypothetical protein, conserved | Pv115405 | 2696.5 | 27 | 4.37122593718338 | 6.21915217491581 | MAL13P1.347 | 8.4 | 3573 | 0.3027027027027027 | PY04251 | 2042 | 920 | 490 | 1.98704103671706
G10 | G10 protein, putative | Pv080110 | 2499.25 | 32 | 13.4948704103671 | 14.8200308349146 | PFE1140c | 7.8 | 3614 | 0.1444635533800767 | PY07622 | 1337 | 214 | 1900 | 1.5
UIS2 | UIS2, Ser/Thr protein phosphatase | Pv117230 | 2332.2 | 34 | 9.42207857792142 | 11.5113524185587 | [NAME] | 802.6 | 206 | 1.80082056542086 | PY04404 | 470 | 5024 | 30 | 27.9111111111111
UVS4 | hypothetical protein, conserved | Pv091250 | 2272.75 | 36 | 2.06928732387954 | 4.00110909635054 | PF11_0126 | 3485.5 | 27 | 1.798298145206761
UVS5 | hypothetical protein, conserved | Pv122540 | 2021.7 | 47 | 4.83111296971145 | 6.85066585341059 | PF13_0081 | 36.3 | 2358 | 1.010137149672033
UVS6 | adenosine/AMP deaminase, putative | Pv082415 | 1835.35 | 56 | 14.2773239984441 | 14.9203316803511 | MAL13P1.146 | 114.6 | 1100 | 1.70065719737121 | PY01044 | 79 | 34.6666666666666 | 4607 | 0.693333333333333
UVS7 | hypothetical protein, conserved | Pv086190 | 1785.15 | 58 | 4.56064380149453 | 4.99244903095897 | PF14_0046 | 120.4 | 1053 | 1.471240289779174
UVS8 | heat shock protein, putative | Pv122065 | 1702.95 | 65 | 13.3486184597295 | 14.9762553865095 | PF13_0021 | 0 | 4318 | 0
[NAME] | hypothetical protein, conserved in Plasmodium species|hypothetical protein, conserved|missing gene in syntenic region|correct Pf, Pk orthologs | Pv096306 | 1497.1 | 74 | 12.9394987035436 | 11.0097073098985 | MAL7P1.105 | 4.5 | 3837 | 0.45
UVS10 | hypothetical protein, conserved | Pv122570 | 1471.05 | 75 | 10.6501357466063 | 6.34428774744469 | MAL13P1.77 | 1369.2 | 142 | 0.7866575834926993 | PY04620 | 36 | 37 | 4483 | 0.850574712643678
UVS11 | LisH domain protein, putative | Pv122010 | 1345.95 | 82 | 4.54137494727962 | 4.1457216780632 | PF13_0018 | 22.3 | 2873 | 0.4470217640320733 | PY02930 | 105 | 188.333333333333 | 1772 | 0.467908902691511
UVS12 | hypothetical protein, conserved | Pv092045 | 1295 | 88 | 5.54069954005776 | 5.15874596661753 | PF11_0296 | 61 | 1777 | 1.515527950310559 | PY03468 | 202 | 138 | 2224 | 0.542239685658153
UVS13 | hypothetical protein, conserved | Pv117560 | 1256.3 | 92 | 5.20099358310908 | 4.76991419242159 | [NAME] | 0 | 4344 | 0
UVS14 | hypothetical protein, conserved | Pv098610 | 1183.6 | 101 | 2.69198840052311 | 4.37107615038038 | PFI0160w | 11.7 | 3359 | 0.4216216216216216 | PY01109 | 63 | 71 | 3376 | 0.442367601246105
UVS15 | hypothetical protein, conserved | Pv085765 | 1169.6 | 103 | 6.31022390072835 | 4.39831528279181 | PF14_0135 | 23.8 | 2817 | 0.5896301539550521 | PY01402 | 198 | 194.333333333333 | 1716 | 2.662100456621
UVS16 | alternative splicing factor ASF-1, putative | Pv091625 | 1021 | 132 | 3.24668097623022 | 5.31687757121283 | PF11_0205 | 0 | 4329 | 0 | PY01659 | 541 | 52 | 3949 | 0.141112618724559
UVS17 | hypothetical protein, conserved | [NAME] | 1018.5 | 133 | 5.39745627980922 | 5.78430258973194 | PF13_0357 | 19.6 | 2978 | 0.9931234165761853 | PY05706 | 27 | 29 | 4859 | 0.674418604651162
UVS18 | WD domain, G-beta repeat domain containing protein | Pv092555 | 1016.05 | 134 | 3.86771983250856 | 4.73948129489691 | PF11_0399 | 979 | 180 | 9.177715280567833 | PY05176 | 39 | 1861.66666666666 | 279 | 43.2945736434108
UVS19 | AP2 transcription factor, putative | Pv081810 | 943.5 | 144 | 6.19094488188976 | 6.00190839694656 | PF07_0126 | 318.5 | 452 | 0.6029260641462492 | PY01583 | 2796 | 1128 | 441 | 5.1156462585034
UVS20 | hypothetical protein, conserved | Pv101250 | 900.4 | 157 | 5.77087005287614 | 5.13282407935241 | PFL2260w | 9.1 | 3519 | 0.1578490893321769 | PY00402 | 165 | 81 | 3165 | 3
UVS21 | hypothetical protein, conserved | Pv003740 | 890.5 | 158 | 10.4 | 6.19952659426343 | PFB0270w | 92.3 | 1320 | 0.8148568545844368 | PY05037 | 300 | 560.666666666666 | 723 | 15.574074074074
UVS22 | hypothetical protein, conserved | Pv084545 | 852.55 | 165 | 7.61375306988167 | 7.75397908140063 | PFL0515w | 28.1 | 2662 | 1.903241412675375 | PY07010 | 241 | 47.3333333333333 | 4108 | 0.706467661691542
UVS23 | hypothetical protein, conserved | Pv000570 | 843 | 168 | 3.87052341597796 | 4.44596803966035 | MAL7P1.84 | 244.6 | 593 | 1.029059109895724 | PY04342 | 391 | 295.333333333333 | 1217 | 3.47450980392156
UVS24 | P-type ATPase2, putative | Pv123625 | 794.2 | 180 | 3.94780663601342 | 6.25009837097662 | PFL0950c | 253.2 | 567 | 0.5590197284382835 | PY01853 | 214 | 69.3333333333333 | 3431 | 0.214987080103359
UVS25 | phosphomannomutase, putative | Pv001740 | 779.4 | 187 | 5.35210300429184 | 4.91983335437444 | PF10_0169 | 254.9 | 563 | 0.9974843470483006
UVS26 | hypothetical protein, conserved | Pv085355 | 725.05 | 214 | 3.85408637873754 | 4.05781284978732 | PF14_0215 | 32.2 | 2501 | 0.8679245283018868 | PY00025 | 2637 | 326 | 1124 | 1.99388379204893
UVS27 | hypothetical protein, conserved | Pv111240 | 700 | 224 | 6.4 | 4 | [NAME] | 11 | 3396 | 1.1 | PY02078 | 22 | 186 | 1777 | 12.4
UVS28 | hypothetical protein, conserved | Pv096071 | 647.55 | 238 | 7.21705210365004 | 6.98317696538336 | MAL7P1.141 | 163.6 | 813 | 0.236599349207169
UVS29 | N-acetylglucosamine-1-phosphate transferase, putative | Pv095175 | 605.85 | 266 | 4.0376541152949 | 4.20933787257694 | PFC0935c | 63.4 | 1724 | 0.9017575942294015 | PY00432 | 136 | 170.666666666666 | 1894 | 1.01587301587301
C)
Alias | Description | P. vivax Gene | [NAME] | Rank | Fold Change Spz/ Asex2 | Fold Change Spz/Avg Asexuals | P. falciparum Gene | Spz Exp | Rank | Fold change | P. yoelii [NAME] | Midgut Spz Exp | [NAME] | Rank | Fold Change
DVS1 | phosphatidylinositol N-acetylglucosaminyltransferase protein | Pv098990 | 158.9 | 2331 | 1.25662317121391 | 1.14374145253005 | PFI0535w | 9362.7 | 4 | 4.37548777760345
LSAP-28 | Phist protein, [NAME] Protein-2 | Pv003535 | 94.25 | 4164 | 0.713609691463183 | 0.797849826462372 | PFB0105c | 6534.8 | 8 | 10.4352864687296
DVS2 | hypothetical protein | Pv082765 | 591 | 274 | 0.898722627737226 | 0.845566143016568 | PF13_0203 | 3936.3 | 19 | 4.71695626123427 | PY01502 | 676 | 268 | 1304 | 0.402402402402402
DVS3 | hypothetical protein | Pv082425 | 268.15 | 1073 | 1.11485292589128 | 1.70081187365216 | PF13_0163 | 3555.1 | 23 | 4.85385215525648 | PY01355 | 601 | 2068.66666666666 | 232 | 5.4438596491228
DVS4 | Major Facilitator Superfamily metabolite transporter, putative | Pv003745 | 114.75 | 3469 | 2.50956806998359 | 1.68477462927617 | PFB0275w | 2856.2 | 86 | 8.94759454016558 | PY05039 | 6703 | 4334 | 42 | 16.6692307692307
DVS5 | hypothetical protein, conserved | Pv097905 | 144.75 | 2647 | 0.564382493420411 | 0.836656840645049 | PFE0340c | 2785.8 | 89 | 5.20383737841408 | PY04351 | 575 | 162.333333333333 | 1971 | 0.594627594627594
DVS6 | hypothetical protein, conserved | Pv084470 | 367.85 | 633 | 0.818308214226127 | 1.171646069563 | PFL0440c | 2705.7 | 90 | 9.62613402454829 | PY06576 | 289 | 273 | 1287 | 2.37391304347826
[NAME] | hypothetical protein, conserved | Pv097925 | 82.55 | 4577 | 1.37468776019983 | 0.992664742664742 | PFE0360c | 2694.7 | 91 | 87.9594311028211 | PY04330 | 95 | 35.6666666666666 | 4546 | 1.13227513227513
DVS8 | hypothetical protein, conserved | [NAME] | 137.75 | 2802 | 1.54601571268238 | 1.30519234413492 | PFE0385w | 2148.4 | 104 | 16.5288783865472 | PY02736 | 606 | 444.333333333333 | 873 | 1.04181320828448
[NAME] | p25-alpha domain containing protein | Pv100780 | 71.55 | 4922 | 0.977459016393442 | 0.931276844982428 | PFL1770c | 2080.2 | 106 | 62.1352677618946 | PY05543 | 380 | 144 | 2152 | 3.74025974025974
DVS10 | hypothetical protein, conserved | Pv091065 | 94.15 | 4173 | 1.74351851851851 | 1.31936659192825 | PF11_0091 | 1933.7 | 111 | 4.02327309475686 | PY07039 | 2644 | 836 | 532 | 1.79784946236559
DVS11 | hypothetical protein, conserved | Pv085180 | 76.5999999999999 | 4773 | 1.29064869418702 | 0.969988603267063 | PF14_0250 | 1695.4 | 120 | 4.72717133695803 | PY07137 | 224 | 3780 | 69 | 94.5
DVS12 | hypothetical protein, conserved | Pv099670 | 118.9 | 3337 | 1.94439901880621 | 1.64362731545479 | PFI1230c | 1613.3 | 125 | 161.33 | PY00930 | 3701 | 2637.66666666666 | 154 | 18.1282932416953
DVS13 | hypothetical protein, conserved in Plasmodium species | Pv114891 | 173 | 2088 | 1.7858064516129 | 1.79927197087883 | [NAME] | 1538.7 | 130 | 7.18515059537707
DVS14 | hypothetical protein, conserved | Pv091870 | 149.25 | 2546 | 1.06436084863612 | 0.651234837245833 | PF11_0261 | 1457 | 135 | 7.16674864731923
PDI | Thioredoxin, PDI-like protein, putative | Pv099380 | 230.8 | 1379 | 2.53139566767205 | 1.17647058823529 | PFI0945w | 1407.2 | 140 | 42.3764250376425 | PY05336 | 4465 | 634.666666666666 | 661 | 3.14191419141914
DVS14 | EH (for Eps15 Homology) domain containing protein | Pv119335 | 53.9 | 5287 | 0.657317073170731 | 0.70893068525582 | PFC0190c | 1261.7 | 149 | 4.53348048148243
DVS15 | AP2 domain transcription factor | Pv090110 | 200.65 | 1689 | 1.9409915356711 | 0.994054991330195 | PFD0985w | 1194.8 | 155 | 4.79495485165543 | PY06328 | 680 | 1476.66666666666 | 368 | 3.46635367762128
DVS16 | hypothetical protein, conserved | Pv113915 | 61.7 | 5155 | 1.4813925570228 | 1.15047548014171 | MAL6P1.270 | 1171.3 | 156 | 4.56139082058414 | PY05535 | 41 | 35.3333333333333 | 4566 | 0.686084142394822
DVS17 | hypothetical protein, conserved | Pv100705 | 555.75 | 299 | 0.851985282845316 | 1.17234468937875 | PFL1695c | 1157 | 159 | 34.3250688705234
DVS18 | STAR domain [NAME] binding protein, putative | Pv098995 | 82.1999999999999 | 4591 | 1.53001395998138 | 1.03409233865895 | PFI0540w | 1135.8 | 162 | 12.4024647063411 | PY00335 | 247 | 75.3333333333333 | 3284 | 0.86095238095238
DVS19 | suppressor of Ras1 3-9, putative | Pv089505 | 202.3 | 1665 | 0.933656397830852 | 1.01986287557975 | MAL8P1.69 | 1135.5 | 163 | 0.68362432269717 | PY01841 | 233 | 399.666666666666 | 965 | 0.3054387976054
CyC | cytochrome c, putative | Pv086230 | 150.4 | 2515 | 1.29348527198452 | 1.13304203706493 | PF14_0038 | 1087.2 | 171 | 4.19456003527434
L15 | ribosomal protein L15, putative | Pv085055 | 117 | 3401 | 0.613931523022432 | 0.687507345163944 | PF14_0276 | 1003.9 | 176 | 4.91625857002938 | PY01273 | 480 | 336 | 1099 | 0.734426229508196
ALP1 | ALP1 actin-like protein homolog | Pv091185 | 91.4 | 4285 | 0.9009364218827 | 0.844107868489102 | PF11_0114 | 896.5 | 188 | 8.23988970588235 | PY01744 | 104 | 71 | 3375 | 0.771739130434782
DVS20 | hypothetical protein, conserved | Pv115085 | 54.55 | 5278 | 0.278280831526591 | 0.328159778619984 | MAL13P1.312 | 846.5 | 196 | 60.5879345603272 | PY00978 | 46 | 105.666666666666 | 2688 | 1.66404199475065
CPK4 | calcium-dependent protein kinase 4, putative | Pv002805 | 195.95 | 1752 | 1.53988212180746 | 1.25343823962131 | PFB0665w | 831.2 | 198 | 5.14015636733071 | PY06391 | 4027 | 1832.66666666666 | 289 | 23.0524109014675
DNA Pol | DNA polymerase delta catalytic subunit, putative | Pv001720 | 198.15 | 1722 | 1.44004360465116 | 1.38430906804527 | PF10_0165 | 818.7 | 199 | 5.79933211900424 | PY00203 | 32 | 444.666666666666 | 872 | 5.85087719298245
DVS21 | hypothetical protein, conserved | Pv090035 | 275.5 | 1035 | 1.89054726368159 | 1.47737022737022 | PFD0915w | 808.5 | 202 | 36.9901960784313 | PY05601 | 5182 | 3797 | 67 | 97.3589743589743
SRPR | signal recognition particle receptor, putative | Pv115290 | 129.2 | 3033 | 0.957746478873239 | 0.936367589505725 | PF13_0350 | 790 | 208 | 5.84103512014787 | PY04912 | 134 | 156.333333333333 | 2020 | 1.31372549019607
SCD | stearoyl-CoA desaturase, putative | Pv080675 | 117.1 | 3400 | 0.867728788440163 | 0.736107618808146 | PFE0555w | 770.9 | 213 | 17.2653975363941 | PY04895 | 251 | 380.333333333333 | 999 | 1.18299637117677
DVS22 | CPW_WPC six  cysteine tandem repeats protein | Pv082875 | 277.65 | 1016 | 1.08024511234315 | 0.890646051196509 | MAL13P1.195 | 746.5 | 219 | 10.77644875232
DVS23 | hypothetical protein, conserved | Pv116515 | 392.2 | 564 | 1.85021818610685 | 1.45393883225208 | PF13_0161 | 732.4 | 225 | 7.4702025353344 | PY00261 | 1006 | 409.333333333333 | 933 | 3.36899862825788
E2 | ubiquitin-conjugating enzyme E2 I, putative | Pv113480 | 245.7 | 1245 | 1.49316317228805 | 1.09765904217298 | MAL6P1.65 | 664.9 | 238 | 4.72134307161696 | PY03157 | 87 | 30.6666666666666 | 4788 | 0.601307189542483
[NAME] | dihydrolipoamide acetyltransferase, putative | Pv001900 | 147.05 | 2599 | 1.06500090530508 | 0.754721823034284 | PF10_0407 | 653.6 | 240 | 25.9733181947204 | PY04573 | 242 | 252 | 1371 | 3.6
DVS24 | glycerol kinase, putative | Pv083470 | 81.55 | 4615 | 0.480341628626122 | 0.669485263935637 | PF13_0269 | 637.8 | 244 | 12.0550830295666 | PY00935 | 287 | 27 | 4944 | 0.0163487738419618
DVS25 | [NAME], putative | Pv084580 | 177.1 | 2017 | 1.49105451483898 | 0.948682236983072 | PFL0545w | 631.8 | 247 | 45.36 | PY02427 | 2850 | 777 | 563 | 6.02325581395348
DVS26 | serine/threonine protein kinase, putative | Pv081395 | 319.6 | 793 | 2.87733513391852 | 1.4509465655786 | PFA0380w | 626.1 | 248 | 62.61 | PY06752 | 430 | 5055 | 29 | 53.2105263157894
DVS27 | hypothetical protein, conserved | Pv100610 | 153.35 | 2455 | 0.538211810125471 | 0.567164731119165 | PFL1595w | 625 | 250 | 7.63991967170173 | PY04707 | 467 | 37.3333333333333 | 4462 | 1.24444444444444
D)
Alias | Description | P. vivax Gene | Exp | Rank | Fold Change Spz/Asex2 | Fold Change Spz/All Asexual | P. knowlesi [NAME]
PvSpz1 | hypothetical protein | Pv092255 | 8649 | 5 | 1.34179876121365 | 2.11663130382432
PvSpz2 | hypothetical protein, conserved | Pv113796 | 6201.9 | 8 | 0.984580947051329 | 0.628738963689283
PvSpz3 | hypothetical protein | Pv114765 | 3074.25 | 19 | 6.34585612550314 | 6.26541259909919
PvSpz4 | hypothetical protein | Pv118250 | 2688.3 | 28 | 7.67537473233404 | 5.03870447772384
PvSpz5 | hypothetical protein, conserved in Plasmodium species | Pv091306 | 1978.15 | 50 | 17.3979771328056 | 15.1675356540407 | PKH_091040
PvSpz6 | hypothetical protein | Pv098775 | 1754.3 | 60 | 3.22748597185171 | 3.22327564031896
PvSpz7 | RNA binding protein, L30e domain | Pv099860 | 1548 | 69 | 3.15210751374465 | 4.28002654280026 | PKH_072620
PvSpz8 | hypothetical protein, conserved | Pv087150 | 1322.7 | 85 | 5.0794930875576 | 2.67044880983626
PvSpz9 | hypothetical protein | Pv111505 | 765.9 | 191 | 5.62851368730479 | 3.59966160642947 | PKH_060830
PvSpz10 | hypothetical protein|missing gene in syntenic region | Pv084278 | 696.45 | 226 | 5.14269891083625 | 3.16251929888293 | PKH_130340
PvSpz11 | hypothetical protein | Pv002570 | 564.25 | 291 | 5.35468564650059 | 3.79072892173328 | PKH_040070
PvSpz12 | hypothetical protein | Pv084550 | 558.2 | 296 | 7.06805951250395 | 6.95749719556275 | PKH_130940
PvSpz13 | hypothetical protein | Pv111275 | 543.95 | 305 | 9.13050776332354 | 8.5513284074831 | PKH_061330
PvSpz14 | hypothetical protein | Pv118675 | 542.2 | 308 | 4.76554603383871 | 3.03430522133303 | PKH_127020
PvSpz15 | hypothetical protein | Pv092915 | 537.55 | 313 | 3.07478907478907 | 2.5705336648814 | PKH_094380
PvSpz16 | hypothetical protein | Pv091520 | 534.25 | 320 | 2.14106802925558 | 3.02160511283298
PvSpz17 | Pf52-like protein, Sexual stage antigen s48/45 domain | Pv001015 | 489.3 | 367 | 2.33138773079213 | 2.11689884918231 | PKH_031010
[NAME] | hypothetical protein | Pv121955 | 468.05 | 400 | 2.85570469798657 | 2.87853628536285
PvSpz19 | hypothetical protein | Pv091475 | 429.7 | 468 | 3.27765064836003 | 2.31956815114709 | PKH_091430
References
1 | Kaiser K, [NAME], Camargo N, Ross J, & Kappe SH (2004) Differential transcriptome profiling identifies Plasmodium genes encoding pre-erythrocytic stage-specific proteins. Molecular microbiology 51(5):1221-1232.
2 | Aly AS & Matuschewski K (2005) A malarial cysteine protease is necessary for Plasmodium sporozoite egress from oocysts. The Journal of experimental medicine 202(2):225-230.
3 | Baker RP, Wijetilaka R, & Urban S (2006) Two Plasmodium rhomboid proteases preferentially cleave different adhesins implicated in all invasive stages of malaria. [NAME] pathogens 2(10):e113.
4 | Ishino T, [NAME], Chinzei Y, & Yuda M (2004) Cell-passage activity is required for the malarial parasite to cross the liver sinusoidal cell layer. (Translated from eng) PLoS Biol 2(1):E4 (in eng).
5 | Daubenberger CA, et [NAME]. (2003) Identification and characterization of a conserved, stage-specific gene product of Plasmodium falciparum recognized by parasite growth inhibitory antibodies. Infection and immunity 71(4):2173-2181.
6 | [NAME], et [NAME]. (2002) Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stage. The Journal of biological chemistry 277(44):41948-41953.
7 | Ecker A, Bushell ES, Tewari R, & Sinden RE (2008) Reverse genetics screen identifies six proteins important for malaria development in the mosquito. Molecular microbiology 70(1):209-220.
8 | Siau A, et [NAME]. (2008) Temperature shift and host cell contact up-regulate sporozoite expression of Plasmodium falciparum genes involved in hepatocyte infection. [NAME] pathogens 4(8):e1000121.
9 | Labaied M, Camargo N, & Kappe SH (2007) Depletion of the Plasmodium berghei thrombospondin-related sporozoite protein reveals a role in host cell entry by sporozoites. Molecular and biochemical parasitology 153(2):158-166.
10 | Green JL, [NAME], Grainger M, Knuepfer E, & Holder AA (2006) Plasmodium thrombospondin related apical merozoite protein (PTRAMP) is shed from the surface of merozoites by PfSUB2 upon invasion of erythrocytes. Molecular and biochemical parasitology 150(1):114-117.
11 | Kaiser K, et [NAME]. (2004) A member of a conserved Plasmodium protein family with membrane-attack complex/perforin ([NAME] localizes to the micronemes of sporozoites. Molecular and biochemical parasitology 133(1):15-26.
12 | Ishino T, Chinzei Y, & Yuda M (2005) Two proteins with 6-cys motifs are required for malarial parasites to commit to infection of the hepatocyte. Molecular microbiology 58(5):1264-1275.
13 | Thompson J, Janse CJ, & Waters AP (2001) Comparative genomics in Plasmodium: a tool for the identification of genes and functional analysis. Molecular and biochemical parasitology 118(2):147-154