Spreadsheet: 0afe2010-666f-4b10-8aeb-d7b87a550f8a
Sheet: Sheet1

Supplementary Table 8: Analysis of the 320 protein families inferred by Dollo parsimony to be gained at the ancestor of the Microsporidia (Figure 4 main text). Min_tm and max_tm give the smallest and largest number of transmembrane domains found in proteins of the respective family. Signal peptide predictions (SignalP) are based on T. hominis only. Protein families with no significant similarity (BLASTP cut-off e-value ≤ 0.01, HHsearch e-value cutoff ≤ 0.01 probability > 90%) to existing sequences detected by any method are annotated as Microsporidia-specific novel protein familes. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13 | Unnamed: 14 | Unnamed: 15
T.hominis ORF(s) | Best KOG hits based on BLASTP  (cut-off e-value ≤ 0.01) | KOG category | KOG super-category | T.hominis ORF(s) present in the spore proteomics data | No. of Thom genes in protein cluster | No. of Nce genes in protein cluster | No. of Ebi genes in protein cluster | No. of Ecu genes in protein cluster | No. of Ein genes in protein cluster | Hhsearch for proteins not in KOG (e-value cutoff ≤ 0.01 and a probability > 90%) | Putative T.hominis transporters | min_tm | max_tm | signalp | Microsporidia-specific novel protein family
orf_2670 | KOG2687 Spindle_pole_body_protein,_contains_UNC-84_domain | [D] | Cellular processes and signaling |  | 1 | 1 | 10 | 1 | 1 |  |  | 0 | 1 | 28-28
orf_1271 | [NAME] | [D] | Cellular processes and signaling |  | 1 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_3291 | KOG0987 DNA_helicase_PIF1/RRM3 | [D] | Cellular processes and signaling |  | 1 | 2 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_1027 | [NAME] | [D] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1708 | [NAME] | [D] | Cellular processes and signaling | orf_1708 | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2633 | KOG2011 Sister_chromatid_cohesion_complex_Cohesin,_subunit_STAG/IRR1/SCC3 | [D] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_104 | [NAME] | [D] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1071 | KOG1126 DNA-binding_cell_division_cycle_control_protein | [D] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_390 | [NAME], KOG0118 FOG:_RRM_domain | [D][R] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1618, orf_1684, orf_2792, orf_524 | [NAME] Predicted_mechanosensitive_ion_channel | [M] | Cellular processes and signaling | orf_1684 | 4 | 4 | 4 | 3 | 3 |  | yes | 0 | 6 | 20-26
orf_1922 | [NAME] Predicted_mechanosensitive_ion_channel | [M] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  | yes | 6 | 6 | 23-25
orf_1619, orf_527 | [NAME] Predicted_mechanosensitive_ion_channel | [M] | Cellular processes and signaling | orf_527 | 2 | 1 | 1 | 1 | 1 |  | yes | 4 | 6 | no_sigp
orf_2925 | KOG1550 Extracellular_protein_SEL-1_and_related_proteins | [MOT] | Cellular processes and signaling | orf_2925 | 1 | 1 | 1 | 1 | 1 |  |  | 1 | 1 | 20-26
orf_1691 | KOG3457 Sec61_protein_translocation_complex,_beta_subunit | [O] | Cellular processes and signaling |  | 1 | 0 | 0 | 0 | 1 |  |  | 2 | 2 | no_sigp
orf_1627 | [NAME] Ubiquitin_protein_ligase_RSP5/NEDD4 | [O] | Cellular processes and signaling |  | 1 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_2178 | [NAME] Predicted_E3_ubiquitin_ligase | [O] | Cellular processes and signaling |  | 1 | 0 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1509 | [NAME] Predicted_E3_ubiquitin_ligase | [O] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_3156 | KOG0778 Protease,_Ulp1_family | [O] | Cellular processes and signaling |  | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2640 | KOG2042 Ubiquitin_fusion_degradation_protein-2 | [O] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_924 | [NAME] | [O] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | 17-17
[NAME] | KOG0191 Thioredoxin/protein_disulfide_isomerase | [O] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 1 | 17-19
orf_45 | [NAME]) | [O] | Cellular processes and signaling | orf_45 | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | 18-25
orf_3256 | [NAME]) | [O] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1882 | KOG2014 SMT3/SUMO-activating_complex,_AOS1/RAD31_component | [O] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1912 | KOG2177 Predicted_E3_ubiquitin_ligase | [O] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 1 | no_sigp
orf_22 | [NAME]), [NAME] | [O][R] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | 29-29
orf_646 | [NAME] | [OR] | Cellular processes and signaling | orf_646 | 1 | 0 | 1 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_1443 | [NAME], LSE0039 A_Disease_resistance_protein_(TIR-NBS-LRR_class) | [R][VT] | Cellular processes and signaling |  | 1 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_3228 | [NAME] RasGAP_SH3_binding_protein_rasputin,_contains_NTF2_and_RRM_domains | [T] | Cellular processes and signaling | orf_3228 | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2203 | [NAME] | [T] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1651 | [NAME] Protein_tyrosine_phosphatase,_contains_fn3_domain | [T] | Cellular processes and signaling |  | 1 | 1 | 2 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_562 | [NAME] | [TU] | Cellular processes and signaling |  | 1 | 0 | 0 | 1 | 1 |  |  | 0 | 1 | no_sigp
orf_3078 | KOG4203 Armadillo/beta-Catenin/plakoglobin | [TZ] | Cellular processes and signaling |  | 1 | 0 | 4 | 0 | 1 |  |  | 0 | 0 | no_sigp
orf_179 | KOG0812 SNARE_protein_SED5/Syntaxin_5 | [U] | Cellular processes and signaling |  | 1 | 1 | 0 | 0 | 0 |  |  | 1 | 1 | no_sigp
orf_1345 | KOG3834 Golgi_reassembly_stacking_protein_GRASP65,_contains_PDZ_domain | [U] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1877 | KOG1061 Vesicle_coat_complex_AP-1/AP-2/AP-4,_beta_subunit | [U] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1186 | [NAME] Vacuolar_assembly/sorting_protein_VPS9 | [U] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 1 | no_sigp
orf_2448 | [NAME] SNARE_protein_TLG2/Syntaxin_16 | [U] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 1 | 1 | no_sigp
orf_504 | [NAME] | [U] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 1 | 1 | no_sigp
orf_881 | KOG1691 emp24/gp25L/p24_family_of_membrane_trafficking_proteins | [U] | Cellular processes and signaling | orf_881 | 1 | 1 | 1 | 1 | 1 |  |  | 1 | 2 | 17-22
orf_2650 | [NAME] | [U] | Cellular processes and signaling | orf_2650 | 1 | 1 | 3 | 1 | 1 |  |  | 0 | 2 | 22-29
orf_802 | KOG2218 ER_to_golgi_transport_protein/RAD50-interacting_protein_1 | [UD] | Cellular processes and signaling |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1507 | [NAME]/Nup116) | [YU] | Cellular processes and signaling |  | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_3194 | [NAME]/Nup116) | [YU] | Cellular processes and signaling |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2393 | [NAME]/SPC98_component | [Z] | Cellular processes and signaling |  | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_3247 | [NAME] | [TG] | Cellular processes and signaling / Metabolism |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2161, orf_834 | [NAME] | [TG] | Cellular processes and signaling / Metabolism |  | 2 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2863 | [NAME]specific_nuclease_Dicer_and_related_ribonucleases | [A] | Information storage and processing |  | 1 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_3154 | [NAME] | [A] | Information storage and processing |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2989 | [NAME] | [A] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1495, orf_995 | KOG0306 WD40-repeat-containing_subunit_of_the_18S_rRNA_processing_complex | [A] | Information storage and processing |  | 2 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_139 | KOG1856 Transcription_elongation_factor_SPT6 | [A] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2858 | KOG3322 Ribonucleases_P/MRP_protein_subunit | [A] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_860 | [NAME]) | [AJ] | Information storage and processing | orf_860 | 1 | 0 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2759 | [NAME]/PUF3_and_related_RNA-binding_proteins_(Puf_superfamily) | [J] | Information storage and processing |  | 1 | 1 | 8 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2197 | KOG2315 Predicted_translation_initiation_factor_related_to_eIF-3a | [J] | Information storage and processing |  | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_607 | [NAME] | [J] | Information storage and processing |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 1 | no_sigp
orf_3145 | KOG0401 Translation_initiation_factor_4F,_ribosome/mRNA-bridging_subunit_(eIF-4G) | [J] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1746 | [NAME] Translation_initiation_factor_2B,_epsilon_subunit_(eIF-2Bepsilon/GCD6) | [J] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1327 | KOG0467 Translation_elongation_factor_2/ribosome_biogenesis_protein_RIA1_and_related_proteins | [J] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | 19-19
orf_1445 | [NAME] | [K] | Information storage and processing |  | 1 | 1 | 9 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_163 | KOG2473 RNA_polymerase_III_transcription_factor_(TF)IIIC_subunit | [K] | Information storage and processing |  | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_723 | [NAME] Transcriptional_activator_of_the_JUN_family | [K] | Information storage and processing |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2334 | [NAME]) | [K] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1231 | KOG0384 Chromodomain-helicase_DNA-binding_protein | [K] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1369 | KOG2294 Transcription_factor_of_the_Forkhead/HNF3_family | [K] | Information storage and processing |  | 1 | 2 | 2 | 2 | 2 |  |  | 0 | 0 | no_sigp
orf_68 | [NAME] RNA_polymerase_II_transcription_initiation/nucleotide_excision_repair_factor_TFIIH,_5'-3'_helicase_subunit_RAD3 | [KL] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2024 | [NAME] 5'-3'_exonuclease | [L] | Information storage and processing |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_670 | KOG4751 DNA_recombinational_repair_protein_BRCA2 | [L] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | 43-43
orf_749 | KOG2228 Origin_recognition_complex,_subunit_4 | [L] | Information storage and processing |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_3195 | [NAME] mRNA_splicing_protein_CDC5_(Myb_superfamily) | [AD] | Information storage and processing / Cellular processes and signaling |  | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1482, orf_34 | [NAME] mRNA_splicing_protein_CDC5_(Myb_superfamily), [NAME] Transcription_factor,_Myb_superfamily | [AD][K] | Information storage and processing / Cellular processes and signaling |  | 2 | 3 | 1 | 3 | 3 |  |  | 0 | 0 | no_sigp
orf_1166, orf_2860, orf_2894, orf_2896 | TWOG0263 ATP/ADP_translocase (NTT) | [C] | Metabolism | orf_2860 | 4 | 4 | 4 | 4 | 4 |  | yes | 9 | 12 | 29-29
orf_17 | KOG4513 Phosphoglycerate_mutase | [G] | Metabolism | orf_17 | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2220 | KOG2208 Vigilin | [I] | Metabolism |  | 1 | 0 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2688 | KOG3386 Copper_transporter | [P] | Metabolism |  | 1 | 1 | 0 | 0 | 0 |  |  | 2 | 2 | no_sigp
orf_2669 | [NAME] | [P] | Metabolism |  | 1 | 1 | 0 | 1 | 1 |  | yes | 8 | 12 | 22-44
orf_1807, orf_2247, orf_2461, orf_2966, orf_3025, orf_586, orf_951, orf_952 | KOG0017 FOG:_Transposon-encoded_proteins_with_TYA,_reverse_transcriptase,_integrase_domains_in_various_combinations | [R] | Poorly characterized |  | 8 | 2 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_1623 | TWOG0131 mutator-like_transposase | [R] | Poorly characterized |  | 1 | 0 | 9 | 1 | 1 |  |  | 0 | 1 | no_sigp
orf_303 | KOG0274 Cdc4_and_related_F-box_and_WD-40_proteins, [NAME] WD40_repeat_protein | [R] | Poorly characterized |  | 1 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_614 | KOG2979 Protein_involved_in_DNA_repair | [R] | Poorly characterized |  | 1 | 0 | 1 | 1 | 0 |  |  | 0 | 1 | no_sigp
orf_2997 | KOG1844 PHD_Zn-finger_proteins | [R] | Poorly characterized |  | 1 | 1 | 0 | 1 | 0 |  |  | 0 | 0 | no_sigp
orf_1020, orf_1021 | [NAME] | [R] | Poorly characterized |  | 2 | 0 | 1 | 0 | 0 |  |  | 1 | 2 | 24-27
orf_497 | KOG0490 Transcription_factor,_contains_HOX_domain | [R] | Poorly characterized |  | 1 | 0 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_612 | [NAME] Predicted_chitinase | [R] | Poorly characterized | orf_612 | 1 | 0 | 1 | 1 | 1 |  |  | 0 | 0 | 20-21
orf_1085 | KOG1539 WD_repeat_protein | [R] | Poorly characterized |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 1 | no_sigp
orf_1683 | TWOG0932 Zn-finger-containing_protein | [R] | Poorly characterized |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_362 | KOG1293 Proteins_containing_armadillo/beta-catenin-like_repeat | [R] | Poorly characterized |  | 1 | 1 | 0 | 1 | 1 |  |  | 0 | 0 | no_sigp
[NAME], orf_2286, orf_2829 | KOG1075 FOG:_Reverse_transcriptase | [R] | Poorly characterized |  | 3 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_1160, orf_2304, orf_59 | KOG1075 FOG:_Reverse_transcriptase | [R] | Poorly characterized |  | 3 | 1 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_2488 | [NAME] | [R] | Poorly characterized |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2959 | KOG4840 Predicted_hydrolases_or_acyltransferases_(alpha/beta_hydrolase_superfamily) | [R] | Poorly characterized |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_2517 | [NAME] Protein_kinase | [R] | Poorly characterized |  | 1 | 1 | 1 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_1804 | KOG2602 Predicted_cell_surface_protein_homologous_to_bacterial_outer_membrane_proteins | [R] | Poorly characterized |  | 1 | 1 | 2 | 1 | 1 |  |  | 0 | 0 | no_sigp
orf_837 | KOG1675 Predicted_cyclin | [R] | Poorly characterized |  | 1 | 1 | 3 | 1 | 1 |  |  | 0 | 2 | no_sigp
orf_1121 | KOG4679 Uncharacterized_protein_PSP1_(suppressor_of_DNA_polymerase_alpha_mutations_in_yeast) | [R] | Poorly characterized |  | 1 | 2 | 0 | 2 | 2 |  |  | 0 | 0 | no_sigp
orf_1157, [NAME], [NAME], orf_2458, orf_2777, orf_2854, orf_3023, orf_954 | KOG0017 FOG:_Transposon-encoded_proteins_with_TYA,_reverse_transcriptase,_integrase_domains_in_various_combinations, LSE3957 C_Unnamed_protein | [R][X] | Poorly characterized |  | 8 | 5 | 0 | 0 | 0 |  |  | 0 | 0 | no_sigp
orf_1005 | KOG4051 Uncharacterized_conserved_protein | [S] | Poorly characterized |  | 1 | 0 | 1 | 0 | 0 | pfam07904 CT20 CT20 family. The S. cerevisiae member of this family is part of NuA4, the only essential histone acetyltransferase complex in Saccharomyces cerevisiae involved in global histone acetylation. Probab=98.40  E-value=6.2e-10 |  | 0 | 0 | no_sigp
orf_2489 | KOG2318 Uncharacterized_conserved_protein | [S] | Poorly characterized |  | 1 | 1 | 0 | 0 | 0 | [NAME] conserved protein (Probab=97.99  E-value=2.1e-08), NUC153 domain;  InterPro: IPR012580 This small domain is found in a novel nucleolar family.; GO: 0005634 nucleus Probab=95.59  E-value=0.0012 |  | 0 | 0 | no_sigp
orf_611 | KOG3237 Uncharacterized_conserved_protein | [S] | Poorly characterized |  | 1 | 0 | 0 | 1 | 1 | PF03998 Utp11:  Utp11 protein;  InterPro: IPR007144 A large ribonuclear protein complex is required for the processing of the small-ribosomal-subunit rRNA - the small-subunit (SSU) processome. (Probab=99.22  E-value=9.1e-15) |  | 0 | 0 | no_sigp
orf_2687 | KOG1106 Uncharacterized_conserved_protein | [S] | Poorly characterized |  | 1 | 0 | 1 | 1 | 1 | 2e9x_C GINS complex subunit 3; eukaryotic DNA replication; HET: DNA; 2.30A {Homo sapiens} SCOP: a.278.1.3 d.344.1.4 PDB: 2eho_D* 2q9q_D* (Probab=99.15  E-value=3.8e-14) |  | 0 | 0 | no_sigp
orf_192 | KOG4784 Uncharacterized_conserved_protein | [S] | Poorly characterized |  | 1 | 1 | 0 | 1 | 1 | pfam09814 DUF2351 Uncharacterized conserved protein (DUF2351). Members of this family of proteins have no known function. (Probab=98.52  E-value=1.8e-10) |  | 0 | 1 | no_sigp
orf_2492 | KOG4534 Uncharacterized_conserved_protein | [S] | Poorly characterized |  | 1 | 1 | 0 | 1 | 1 | pfam08214 KAT11 Histone acetylation protein. Histone acetylation is required in many cellular processes including transcription, DNA repair, and chromatin assembly. This family contains the fungal KAT11 protein (previously known as RTT109) which is required for [NAME] acetylation. Loss of KAT11 results in the loss of [NAME] acetylation, both on bulk histone and on chromatin. KAT11 and [NAME] acetylation appear to correlate with actively transcribed genes and associate with the elongating form of Pol II in yeast. This family also incorporates the p300/CBP histone acetyltransferase domain which has different catalytic properties and cofactor regulation to KAT11. (Probab=99.33  E-value=8e-16) |  | 0 | 0 | no_sigp
orf_1317 | [NAME]; Blast hits to Microsporidia, Firmicutes, Ascomycota, Chordata, [NAME], Cnidaria, [NAME] | [X] | Poorly characterized |  | 1 | 1 | 0 | 0 | 0 | No hit at cutoff evalue ≤ 0.01 |  | 0 | 0 | no_sigp
orf_780 | LSE2351 E_Unnamed_protein; Blast hits to only Microsporidia | [X] | Poorly characterized |  | 1 | 1 | 15 | 2 | 2 | No hit at cutoff evalue ≤ 0.01 |  | 0 | 2 | no_sigp
orf_2549, orf_2552, orf_2553, orf_2707, orf_2848, orf_2851 | [NAME]; Blast hits to [NAME], Firmicutes, Microsporidia, Proteobacteria, Actinobacteria | [X] | Poorly characterized | orf_2553 | 6 | 8 | 1 | 4 | 2 | d1qxma1 b.42.2.1 (A:4-148) Hemagglutinin component Ha1 {Clostridium botulinum D phage [TaxId: 29342]} (Probab=97.46  E-value=7.8e-07) |  | 0 | 1 | 15-37
orf_2765 | TWOG0997 Unnamed_protein; Blast hits to Microsporidia, Ascomycota, Basidiomycota | [X] | Poorly characterized |  | 1 | 1 | 1 | 1 | 1 | PF02200 STE:  STE like transcription factor;  InterPro: IPR003120 This family consists of transcription factors related to STE and is found associated with the C2H2 zinc finger in some proteins.; GO: 0003700 sequence-specific DNA binding transcription factor activity, 0006355 regulation of transcription, DNA-dependent, 0005634 nucleus Probab=100.00  E-value=0 |  | 0 | 0 | no_sigp
orf_1322 | TWOG0266 Unnamed_protein;                            Blast  hits to Microsporidia, Placozoa, Apicomplexa, Ascomycota, [NAME], Basidiomycota, [NAME], Bacillariophyta, [NAME] | [X] | Poorly characterized |  | 1 | 1 | 1 | 1 | 1 | 2roh_A RTBP1, telomere binding protein-1; plant, nucleus, DNA binding protein; NMR {Oryza sativa} (Probab=98.59  E-value=9.1e-11) |  | 0 | 0 | no_sigp
orf_675, orf_3044 | LSE3879 [NAME];                  Blast Blast hits to [NAME], Microsporidia, Chordata | [X] | Poorly characterized | orf_3044 | 2 | 0 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | 27-27
orf_3092 | [NAME]; Blast taxa hit only Microsporidia; | [X] | Poorly characterized |  | 1 | 2 | 0 | 2 | 2 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp
orf_1803, orf_1997, orf_892 | LSE4146 E_Unnamed_protein;                            Blast hits to [NAME], Microsporidia, Nematoda, [NAME], Cnidaria | [X] | Poorly characterized |  | 3 | 2 | 0 | 2 | 2 | PF00620 RhoGAP:  RhoGAP domain;  InterPro: IPR000198 Members of the [NAME] family of small G proteins transduce signals from plasma-membrane receptors and control cell adhesion, motility and shape by [NAME] formation. Like all other GTPases, Rho proteins act as molecular switches, with an active GTP-bound form and an inactive GDP-bound form.  (Probab=99.45  E-value=4.4e-17) |  | 0 | 0 | no_sigp
orf_3144 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | 26-26 | Yes
orf_2186 | Blast hits to [NAME], Hemichordata, Microsporidia, Placozoa, Intramacronucleata, Ascomycota, [NAME], Basidiomycota, Cnidaria, [NAME], Streptophyta | NOT in KOG |  |  | 1 | 1 | 7 | 1 | 1 | TIGR00272 DPH2 diphthamide biosynthesis protein 2 (Probab=99.22  E-value=9.3e-15) |  | 0 | 0 | no_sigp
orf_2528 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | 24-24 | Yes
orf_1665 | Blast hits to Arthropoda, Microsporidia | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | PRK04036 DNA polymerase II small subunit; Validated (Probab=99.21  E-value=1.1e-14) |  | 0 | 0 | no_sigp
orf_2629 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | pfam12253 CAF1A Chromatin assembly factor 1 subunit A.  Probab=98.22  E-value=3.4e-09 |  | 0 | 0 | no_sigp
orf_1396 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1396 | 1 | 1 | 2 | 1 | 1 | pfam10455 BAR_2 Bin/amphiphysin/Rvs domain for vesicular trafficking. This [NAME] entry includes proteins that are not matched by pfam03114. Probab=99.18  E-value=2.1e-14 |  | 0 | 0 | no_sigp
orf_1750 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | pfam10198 Ada3 Histone acetyltransferases subunit 3. Ada3 is a family of proteins conserved from yeasts to humans. It is an essential component of the Ada transcriptional coactivator (alteration/deficiency in activation) complex.  Probab=94.76  E-value=0.0052 |  | 0 | 0 | no_sigp
orf_970 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | [NAME] domain. MAN1 is an integral protein of the inner nuclear membrane which binds to chromatin associated proteins and plays a role in nuclear organisation. The C terminal nucleoplasmic region forms a DNA binding winged helix and binds to [NAME]. This C-terminal tail is also found in S. cerevisiae and is thought to consist of three conserved helices followed by two downstream strands.Probab=99.85  E-value=5.6e-26 |  | 2 | 2 | no_sigp
orf_2428 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 1 | 2 | 1 | 1 | pfam08286 Spc24 Spc24 subunit of Ndc80. Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation. Probab=98.38  E-value=7.6e-10 |  | 0 | 0 | no_sigp
orf_2631 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | pfam06870 RNA_pol_I_A49 A49-like [NAME] polymerase I associated factor. Saccharomyces cerevisiae A49 is a specific subunit associated with [NAME] polymerase I (Pol I) in eukaryotes.  Probab=98.54  E-value=1.4e-10 |  | 0 | 1 | no_sigp
orf_304 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | pfam05773 RWD RWD domain. This domain was identified in WD40 repeat proteins and [NAME] finger domain proteins. The function of this domain is unknown.  [NAME] |  | 0 | 0 | no_sigp
orf_1724 | Blast hits to Microsporidia, Parabasalia | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | pfam05327 RRN3 RNA polymerase I specific transcription initiation factor [NAME]. This family consists of several eukaryotic proteins which are homologous to the yeast RRN3 protein. RRN3 is one of the RRN genes specifically required for the transcription of rDNA by [NAME] polymerase I (Pol I) in Saccharomyces cerevisiae. Probab=99.53  E-value=3.7e-18 |  | 0 | 0 | no_sigp
orf_594 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | pfam04177 TAP42 TAP42-like family. The TOR signalling pathway activates a cell-growth program in response to nutrients. [NAME] (pfam04176) interacts with TAP42 and negatively regulates the TOR signaling pathway. Probab=98.50  E-value=2.3e-10 |  | 0 | 0 | no_sigp
orf_2622 | Blast hits to Microsporidia, Chordata | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | pfam04130 Spc97_Spc98 Spc97 / Spc98 family. The spindle pole body (SPB) functions as the microtubule-organising centre in yeast. Members of this family are spindle pole body (SBP) components such as Spc97 and Spc98 that form a complex with gamma-tubulin. This family of proteins includes the grip motif 1 and grip motif 2. Probab=99.84  E-value=1.6e-25 |  | 0 | 0 | no_sigp
orf_2701 | Blast hits to [NAME], Microsporidia, Ascomycota, Chordata,                                  Basidiomycota, Cnidaria | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | pfam04097 Nic96 Nup93/Nic96. Nup93/Nic96 is a component of the nuclear pore complex. It is required for the correct assembly of the nuclear pore complex. In Saccharomyces cerevisiae, Nic96 has been shown to be involved in the distribution and cellular concentration of the GTPase Gsp1. The structure of Nic96 has revealed a mostly alpha helical structure. Probab=99.70  E-value=4e-21 |  | 0 | 0 | no_sigp
orf_1772 | Blast hits to [NAME], Microsporidia, Hemichordata, Chordata | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | pfam03999 MAP65_ASE1 [NAME] associated protein (MAP65/ASE1 family). Probab=99.36  E-value=4.8e-16 |  | 0 | 0 | no_sigp
orf_2255 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2255 | 1 | 1 | 1 | 1 | 1 | pfam03399 SAC3_GANP SAC3/GANP/Nin1/mts3/eIF-3 p25 family. This large family includes diverse proteins involved in large complexes. The alignment contains one highly conserved negatively charged residue and one highly conserved positively charged residue that are probably important for the function of these proteins. The family includes the yeast nuclear export factor [NAME], and mammalian GANP/MCM3-associated proteins, which facilitate the nuclear localisation of MCM3, a protein that associates with chromatin in the G1 phase of the cell-cycle. Probab=96.35  E-value=0.00014 |  | 0 | 0 | no_sigp
orf_1526 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | PF12923 RRP7:  Ribosomal RNA-processing protein 7 (RRP7) Probab=95.40  E-value=0.0017 |  | 0 | 0 | no_sigp
orf_1365 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | No hit at cutoff evalue < 0.01. |  | 0 | 0 | no_sigp | Yes
orf_135 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | PF11699 Mif2:  Mif2/CENP-C like; PDB: 2vpv_A. Probab=97.50  E-value=6.1e-07 |  | 0 | 0 | no_sigp
orf_2363 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | PF11571 Med27:  Mediator complex subunit 27;  InterPro: IPR021627  Mediator is a large complex of up to 33 proteins that is conserved from plants to fungi to humans - the number and representation of individual subunits varying with species.  Probab=94.41  E-value=0.0083 |  | 0 | 0 | 25-25
orf_2810 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue ≤ 0.01. |  | 0 | 0 | no_sigp | Yes
orf_591 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 4 | 0 | 1 | PF11221 Med21:  Subunit 21 of Mediator complex;  InterPro: IPR021384 The Mediator complex is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.  (Probab=93.91  E-value=0.01) |  | 0 | 0 | no_sigp
orf_713 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | PF10382 DUF2439:  Protein of unknown function (DUF2439);  InterPro: IPR018838 This domain is found at the N-terminal of proteins implicated in telomere maintenance in Saccharomyces cerevisiae ([NAME]'s yeast) and in meiotic chromosome segregation in Schizosaccharomyces pombe (Fission yeast). Probab=97.75  E-value=1.2e-07 |  | 0 | 0 | no_sigp
orf_2177, orf_2176, orf_895 | Blast hits to [NAME], Microsporidia, Ascomycota | NOT in KOG |  |  | 3 | 1 | 0 | 1 | 1 | PF10296 DUF2404:  Putative integral membrane protein conserved region (DUF2404);  InterPro: IPR019411  This is entry represents a conserved unknown function domain which is found in mitochondrial distribution and morphology proteins.  Probab=96.37  [NAME] |  | 0 | 1 | 16-27
orf_2826 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | PF09807 DUF2348:  Uncharacterized conserved protein (DUF2348);  InterPro: IPR018627  Members of this family of putative uncharacterised proteins have no known function.  Probab=96.07  E-value=0.00033 |  | 0 | 0 | no_sigp
orf_2865 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF09797 NatB_MDM20:  N-acetyltransferase B complex (NatB) non catalytic subunit;  InterPro: IPR019183  This is the non-catalytic subunit of the N-terminal acetyltransferase B complex (NatB). The NatB complex catalyses the acetylation of the amino-terminal methionine residue of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met. In Saccharomyces cerevisiae ([NAME]'s yeast) this subunit is called MDM20 and in Schizosaccharomyces pombe (Fission yeast) it is called Arm1.  Probab=94.94  E-value=0.0039 |  | 0 | 0 | no_sigp
orf_1290 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | PF09770 [NAME]:  Topoisomerase II-associated protein [NAME];  InterPro: IPR019167  Proteins in this entry are necessary for accurate chromosome transmission during cell division.  [NAME]  E-value=5.2e-11 |  | 0 | 0 | no_sigp
orf_1878 | Blast hits to Microsporidia, [NAME], Ascomycota, Basidiomycota | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF09763 Sec3:  Exocyst complex component Sec3; This entry represents the subunit Exoc1 (Sec3). Sec3 binds to the C-terminal cytoplasmic domain of GLYT1 (glycine transporter protein 1). Sec3 is the exocyst component that is closest to the plasma membrane docking site and it serves as a spatial landmark in the plasma membrane for incoming secretory vesicles. Sec3 is recruited to the sites of polarised membrane growth through its interaction with Rho1p, a small GTP-binding protein.  Probab=99.93  E-value=5.5e-31 |  | 0 | 0 | no_sigp
orf_2199 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF09637 Med18:  Med18 protein;  InterPro: IPR019095 The Mediator complex is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes.  Probab=97.02  E-value=8.6e-06 |  | 0 | 0 | no_sigp
orf_1632 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 5 | 0 | 0 | PF09340 NuA4:  Histone acetyltransferase subunit NuA4;  InterPro: IPR015418 The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. NuA4 complexes are highly conserved in eukaryotes and play primary roles in transcription, cellular response to DNA damage, and cell cycle control.  Probab=96.87  E-value=1.7e-05 |  | 0 | 0 | no_sigp
orf_3113 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_3113 | 1 | 1 | 0 | 1 | 1 | PF09090 MIF4G_like_2:  MIF4G like;  InterPro: IPR015174 This entry represents an MIF4G-like domain. MIF4G domains share a common structure but can differ in sequence. This entry is designated "type 2", and is found in nuclear cap-binding proteins and eIF4G. The MIF4G domain is a structural motif with an ARM (Armadillo) repeat-type fold, consisting of a 2-layer alpha/alpha right-handed superhelix.  Probab=97.12  E-value=5.1e-06 |  | 0 | 0 | no_sigp
orf_3288 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | PF08700 Vps51:  Vps51/Vps67;  InterPro: IPR014812 The VFT tethering complex (also known as GARP complex, Golgi associated retrograde protein complex, Vps53 tethering complex) is a conserved eukaryotic docking complex which is involved in recycling of proteins from endosomes to the late Golgi. Vps51 (also known as Vps67) is a subunit of VFT and interacts with the SNARE Tlg1 (Probab=92.84  E-value=0.037 ) |  | 0 | 0 | no_sigp
orf_1070 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cut-off evalue < 0.01 |  | 0 | 0 | no_sigp | Yes
orf_1293 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | [NAME] GIT_SHD:  Spa2 homology domain (SHD) of GIT;  InterPro: IPR013724 [NAME] proteins are signalling integrators with GTPase-activating function which may be involved in the organisation of the cytoskeletal matrix assembled at active zones (CAZ). The function of the CAZ might be to define sites of neurotransmitter release. Mutations in the Spa2 homology domain (SHD) domain of  GIT1 described here interfere with the association of GIT1 with Piccolo, beta-PIX, and focal adhesion kinase.  Probab=95.51  E-value=0.0014 |  | 0 | 0 | no_sigp
orf_525 | Blast hits to [NAME], Microsporidia, Nematoda, [NAME], Euryarchaeota, [NAME] | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF08423 Rad51:  Rad51;  InterPro: IPR013632 This domain is found at the C terminus of the DNA repair and recombination protein Rad51. It is critical for DNA binding. Rad51 is a homologue of the bacterial RecA protein. Rad51 and RecA share a core ATPase domain.; Probab=99.76  E-value=1.1e-22 |  | 0 | 0 | no_sigp
orf_3006 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_3006 | 1 | 1 | 1 | 1 | 1 | PF08389 Xpo1:  Exportin 1-like protein;  InterPro: IPR013598 Exportin 1 is a nuclear export receptor that translocates proteins out of the nucleus; it interacts with leucine-rich nuclear export signal (NES) sequences in proteins to be transported, as well as with RanGTP.  Probab=95.66  E-value=0.00097 |  | 0 | 0 | no_sigp
orf_2927 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF07524 Bromo_TP:  Bromodomain associated;  InterPro: IPR006565 This bromodomain is found in eukaryotic transcription factors and PHD domain containing proteins (IPR001965 from INTERPRO). The tandem PHD finger-bromodomain is found in many chromatin-associated proteins. It is involved in gene silencing by the human co-repressor KRAB-associated protein 1 (KAP1). Probab=96.83  E-value=2e-05 |  | 0 | 1 | no_sigp
orf_1448 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | PF07393 Sec10:  Exocyst complex component Sec10;  InterPro: IPR009976 This family contains the Sec10 component (approximately 650 residues long) of the eukaryotic exocyst complex, which specifically affects the synthesis and delivery of secretory and basolateral plasma membrane proteins. Probab=99.00  E-value=4e-13 |  | 0 | 0 | no_sigp
orf_3263 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | PF07202 Tcp10_C:  T-complex protein 10 C-terminus;  InterPro: IPR009852 Proteins in this entry include T-complex 10, involved in spermatogenesis in mice, and centromere protein J, which not only inhibits microtubule nucleation from the centrosome, but also depolymerises taxol-stabilised microtubules. Probab=97.64  E-value=2.6e-07 |  | 0 | 0 | no_sigp
orf_2071 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 0 | 0 | PF07159 DUF1394:  Protein of unknown function (DUF1394) (Probab=94.95  E-value=0.0039) |  | 0 | 0 | no_sigp
orf_3284 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_3284 | 1 | 1 | 2 | 1 | 1 | PF05907 DUF866:  Eukaryotic protein of unknown function (DUF866);  InterPro: IPR008584 This family consists of a number of hypothetical eukaryotic proteins of unknown function with an average length of around 165 residues.; PDB: 1zso_B. Probab=98.72  E-value=1.9e-11 |  | 0 | 0 | no_sigp
orf_774 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF05859 Mis12:  Mis12 protein;  InterPro: IPR008685 Kinetochores are the chromosomal sites for spindle interaction and play a vital role for chromosome segregation. Fission Saccharomyces cerevisiae kinetochore protein Mis12, is required for correct spindle morphogenesis, determining metaphase spindle length. Probab=97.00  E-value=9.7e-06 |  | 0 | 0 | no_sigp
orf_2032 | Blast hits to Microsporidia, Ascomycota, Chordata, Hemichordata, Cnidaria, [NAME], Basidiomycota, [NAME] | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF05793 TFIIF_alpha:  Transcription initiation factor IIF, alpha subunit (TFIIF-alpha);  InterPro: IPR008851 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) or RNA polymerase II-associating protein 74 (RAP74) is the large subunit of transcription factor IIF (TFIIF), which is essential for accurate initiation and stimulates elongation by RNA polymerase II. Probab=98.72  E-value=2e-11 |  | 0 | 0 | no_sigp
orf_2282 | Blast hits to [NAME], Microsporidia, Apicomplexa, Nematoda, [NAME], Basidiomycota, Cnidaria, Echinodermata | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF04959 ARS2:  Arsenite-resistance protein 2;  InterPro: IPR007042 This entry represents Arsenite-resistance protein 2 (also known as Serrate RNA effector molecule homolog) which is thought to play a role in arsenite resistance, although does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Probab=97.42  E-value=1e-06 |  | 0 | 0 | no_sigp
orf_2924 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2924 | 1 | 0 | 0 | 1 | 1 | PF04478 Mid2:  Mid2 like cell wall stress sensor (Probab=93.16  E-value=0.029) IPR007567 This family represents a region near the C terminus of Mid2, which contains a transmembrane region.The remainder of the protein sequence is serine-rich and of low complexity, and is therefore impossible to align accurately. Mid2 is thought to act as a mechanosensor of cell wall stress. The C-terminal cytoplasmic region of Mid2 is known to interact with Rom2, a guanine nucleotide exchange factor (GEF) for [NAME], which is part of the cell wall integrity signalling pathway. ??? TIGR01167 (Probab=99.42  E-value=1.4e-16) LPXTG-motif cell wall anchor domain. TIGR03304 (Probab=99.05  E-value=2.7e-13) outer membrane insertion C-terminal signal. TIGR03057 (Probab=97.90  E-value=6e-08) X-X-X-Leu-X-X-Gly heptad repeats |  | 1 | 1 | 27-27
orf_224 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | PF04109 APG9:  Autophagy protein Apg9 ;  InterPro: [NAME] is a bulk degradation process induced by starvation in eukaryotic cells. In yeast, 15 Apg proteins coordinate the formation of autophagosomes. No molecule involved in autophagy has yet been identified in higher eukaryotes. Probab=99.69  E-value=7.1e-21 |  | 4 | 5 | 23-26
orf_2046 | Blast hits to Microsporidia, Basidiomycota, Ascomycota | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF04048 Sec8_exocyst:  Sec8 exocyst complex component specific domain;  InterPro: [NAME] is a component of the exocyst complex involved in the docking of exocystic vesicles with a fusion site on the plasma membrane.  Probab=98.56  E-value=1.2e-10 |  | 0 | 0 | no_sigp
orf_1542 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 0 | 1 | PF04004 Leo1:  Leo1-like protein;  InterPro: IPR007149 Members of this family are part of the Paf1/RNA polymerase II complex. The Paf1 complex probably functions during the elongation phase of transcription (Probab=88.28  E-value=0.27) |  | 0 | 0 | no_sigp
orf_2445 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | PF03914 CBF:  CBF/[NAME] family;  InterPro: IPR005612 This domain is present in the CAATT-binding protein which is essential for growth and necessary for [NAME] ribosomal subunit biogenesis. Other proteins containing this domain stimulate transcription from the HSP70 promoter. Probab=96.98  [NAME] |  | 0 | 0 | no_sigp
orf_912 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 10 | 1 | 1 | PF03909 BSD:  BSD domain  ;  InterPro: IPR005607 The BSD domain is an about 60-residue long domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found. Additionally, it is also found in several hypothetical proteins.  Probab=82.14  E-value=0.98 |  | 0 | 0 | no_sigp
orf_3182 | Blast hits to Microsporidia, Apicomplexa | NOT in KOG |  |  | 1 | 1 | 2 | 1 | 1 | PF03908 Sec20:  Sec20;  InterPro: IPR005606 Sec20 is a membrane glycoprotein associated with secretory pathway. Probab=98.17  E-value=5.1e-09 |  | 0 | 1 | no_sigp
orf_2476 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | PF03813 [NAME]:  [NAME] protein;  InterPro: IPR005554 Members of this family are nucleolar RNA-associated proteins ([NAME]) which are highly conserved from yeast (Saccharomyces cerevisiae) to human. n the mouse, [NAME] is ubiquitously expressed and is specifically localized in the nucleolus. [NAME] is a large nucleolar protein (of more than 1000 amino acids). [NAME] appears to be associated with ribosome biogenesis by interacting with pre-rRNA primary transcript (Probab=98.15  E-value=5.9e-09) |  | 0 | 0 | no_sigp
orf_6 | Blast hits to Microsporidia, Nematoda | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF03661 UPF0121:  Uncharacterised protein family (UPF0121);  InterPro: IPR005344 Uncharacterised integral membrane protein family.; GO: 0016021 integral to membrane [NAME]  E-value=8.8e-12 | yes | 3 | 5 | 24-24
orf_2234 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF01770 Folate_carrier:  Reduced folate carrier;  InterPro: IPR002666 The reduced folate carrier (a transmembrane glycoprotein) transports reduced folate into mammalian cells via the carrier mediated mechanism (as opposed to the receptor mediated mechanism) it also transports cytotoxic folate analogues used in chemotherapy, such as methotrexate (MTX). Probab=99.79  E-value=1.4e-23 | yes | 8 | 12 | no_sigp
orf_1784 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF01105 EMP24_GP25L:  emp24/gp25L/p24 family/GOLD;  InterPro: IPR009038  The GOLD (for Golgi dynamics) domain is a protein module found in several eukaryotic Golgi and lipid-traffic proteins. It is typically between 90 and 150 amino acids long. Most of the size difference observed in the GOLD-domain superfamily is traceable to a single large low-complexity insert that is seen in some versions of the domain.  Probab=97.08  E-value=6.5e-06 |  | 1 | 1 | 17-29
orf_3286 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_3286 | 1 | 1 | 2 | 1 | 1 | PF01105 EMP24_GP25L:  emp24/gp25L/p24 family/GOLD;  InterPro: IPR009038  The GOLD (for Golgi dynamics) domain is a protein module found in several eukaryotic Golgi and lipid-traffic proteins. It is typically between 90 and 150 amino acids long. Most of the size difference observed in the GOLD-domain superfamily is traceable to a single large low-complexity insert that is seen in some versions of the domain.  Probab=92.39  E-value=0.049 |  | 1 | 1 | 17-26
orf_2468 | Blast hits to Cyanobacteria, Microsporidia, Korarchaeota, Aquificae, Actinobacteria, Bacteroidetes/Chlorobi group, Firmicutes, Proteobacteria, Euryarchaeota, Crenarchaeota | NOT in KOG |  | orf_2468 | 1 | 1 | 1 | 1 | 1 | PF00924 MS_channel:  Mechanosensitive ion channel;  InterPro: IPR006685 Mechanosensitive (MS) channels provide protection against hypo-osmotic shock, responding both to stretching of the cell membrane and to membrane depolarisation. Probab=97.62  E-value=2.9e-07 |  | 0 | 0 | no_sigp
[NAME], orf_2257 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2257 | 2 | 0 | 1 | 1 | 1 | PF00566 TBC:  TBC domain;  InterPro: IPR000195 Identification of a TBC domain in GYP6_YEAST and GYP7_YEAST, which are GTPase activator proteins of yeast Ypt6 and Ypt7, imply that these domains are GTPase activator proteins of Rab-like small GTPases. Probab=98.59  E-value=9e-11 |  | 0 | 2 | no_sigp
orf_2735 | Blast hits to Tenericutes, Microsporidia | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | PF00448 SRP54:  SRP54-type protein, GTPase domain (Probab=94.93  E-value=0.004 ).  TIGR03265 - PhnT2 putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (evalue 1E-24), PTHR19222 - ATP-binding cassete (evalue 1e-23) |  | 1 | 1 | 20-33
orf_1513 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1513 | 1 | 0 | 1 | 1 | 1 | PF00335 Tetraspannin:  [NAME] tetradecapeptides family [NAME] family RDS_ROM1 subfamily; Probab=39.03  E-value=30  Score=18.62  Aligned_cols=91  Identities=11%  Similarity=0.254  Sum_probs=50.7 | yes | 3 | 4 | 29-36
orf_916 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 2 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_117, orf_2882 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 2 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 | yes | 4 | 5 | no_sigp | Yes
orf_1554 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_500 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 1 | 1 | no_sigp | Yes
orf_391 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2505 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_314 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_871 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_600 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_768 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_567 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1530 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 1 | 1 | 17-22 | Yes
orf_144 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_169 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1334, orf_1382, orf_150, orf_257, orf_3244, orf_51, orf_52, orf_53 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 8 | 0 | 0 | 2 | 2 | No hit at cutoff evalue < 1 | yes | 2 | 4 | 25-59 | Yes
orf_368 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 10 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1576 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1576 | 1 | 1 | 15 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 18-22 | Yes
orf_2624 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 0 | 0 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1745 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 0 | 0 | No hit at cutoff evalue < 1 |  | 1 | 2 | 20-20 | Yes
orf_2016 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 0 | 0 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_505 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1869 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_3259 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_105 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_3088 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_501 | Blast hits to Microsporidia, Echinodermata | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp
orf_210 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_3208 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_972 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2990 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_742 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_742 | 1 | 1 | 0 | 0 | 1 | No hit at cutoff evalue < 1 |  | 1 | 1 | 22-24 | Yes
orf_2301 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2189 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2020 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_397 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 26-26 | Yes
orf_1364 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1364 | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 17-22 | Yes
orf_1779 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2871 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1109 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | no_sigp | Yes
orf_2275 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2656 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2827 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 22-22 | Yes
orf_1783 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_185 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1785 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_1145 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2732 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | 16-17 | Yes
orf_2446 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 | yes | 3 | 4 | no_sigp | Yes
orf_2196 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 25-25 | Yes
orf_2052 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2825 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | no_sigp | Yes
orf_1274 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 18-23 | Yes
orf_116 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_3293 | Blast hits to Microsporidia, Proteobacteria | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 1 | 1 | 25-25
orf_2878 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 | yes | 5 | 7 | 28-37 | Yes
orf_1333 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2494 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 |  | 2 | 7 | 33-43 | Yes
orf_5 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | no_sigp | Yes
orf_44 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 | yes | 2 | 5 | 25-25 | Yes
orf_1106 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 31-31 | Yes
orf_1735 | Blast hits to [NAME], Microsporidia, Apicomplexa, Ascomycota, Chordata, Proteobacteria, [NAME] | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | no_sigp
orf_3112 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2356 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 1 | 3 | no_sigp | Yes
orf_341 | Blast hits to Microsporidia, Parabasalia | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp
orf_1756 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1756 | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | 16-24 | Yes
orf_2515 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_3203 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_699 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_627 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp
orf_686 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_686 | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 4 | 4 | no_sigp | Yes
orf_2900 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2172 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2172 | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | 18-25 | Yes
orf_152 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_417 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_248 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 0 | no_sigp | Yes
orf_2715, orf_2716 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 2 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 1 | yes | 6 | 8 | 23-24 | Yes
orf_1343, orf_1361 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1361 | 2 | 5 | 0 | 0 | 0 | No hit at cutoff evalue < 1 |  | 0 | 1 | 17-19 | Yes
orf_1575 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1575 | 1 | 2 | 0 | 2 | 2 | No hit at cutoff evalue < 1 |  | 0 | 0 | 17-30 | Yes
orf_1838, orf_1840, orf_1843, orf_1643 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1838, orf_1843, orf_1643 | 4 | 0 | 0 | 2 | 2 | No hit at cutoff evalue < 1 |  | 0 | 0 | 15-27 | Yes
orf_2110, orf_2994, orf_3181 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2110, orf_3181 | 3 | 2 | 1 | 1 | 1 | No hit at cutoff evalue < 1 |  | 0 | 1 | 18-24 | Yes
orf_2317 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | no_sigp | Yes
orf_2632 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | no_sigp | Yes
orf_2037 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | no_sigp | Yes
orf_2971 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2971 | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | no_sigp | Yes
orf_635 | Blast hits to Microsporidia, Placozoa, Chordata | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 0.01 |  | 0 | 0 | no_sigp
orf_610 | Blast hits to Microsporidia, Cnidaria | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff evalue < 0.01 |  | 0 | 0 | no_sigp
orf_1979 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue <  0.01 |  | 0 | 0 | no_sigp | Yes
orf_1287 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff evalue < 0.1 |  | 0 | 0 | no_sigp | Yes
orf_2002 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | No hit at cutoff < 0.1 |  | 0 | 0 | no_sigp | Yes
orf_2045 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | 3aiz_A DNA polymerase sliding clamp B; protein-protein complex, replication; HET: DNA; 2.80A {Sulfolobus tokodaii} PDB: 3aix_B* Probab=92.33  E-value=0.051 |  | 0 | 0 | no_sigp
orf_2614 | Blast hits to [NAME], Microsporidia, Platyhelminthes, Basidiomycota | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | lcl|consensus Probab=98.46  E-value=3.5e-10. PF12895 Apc3:  Anaphase-promoting complex, cyclosome, subunit 3; PDB: 2c2l_D 3kae_A. Probab=93.40  E-value=0.024 |  | 0 | 0 | no_sigp
orf_7 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | lcl|consensus Probab=97.90  E-value=4.1e-08 |  | 0 | 0 | no_sigp
orf_1659 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | lcl|consensus Probab=97.38  E-value=1.3e-06. PF04100 Vps53_N:  Vps53-like, N-terminal ;  InterPro: IPR007234 Vps53 complexes with Vps52 and Vps54 to form a multi-subunit complex involved in regulating membrane trafficking events. Probab=97.19  E-value=3.7e-06 |  | 0 | 0 | 23-23
orf_2756 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2756 | 1 | 1 | 1 | 1 | 1 | smart00553 SEP Domain present in Saccharomyces cerevisiae Shp1, Drosophila melanogaster eyes closed gene (eyc), and vertebrate p47. Probab=95.48  E-value=0.0015. TIGR00264 alpha-NAC-related protein. This hypothetical protein is found so far only in the [NAME].  Probab=94.89  E-value=0.0042 |  | 0 | 0 | no_sigp
orf_2048 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | [NAME] beta'-subunit; beta-propeller, alpha-solenoid, transport protein; 2.50A {Saccharomyces   cerevisiae}   Probab=99.96  E-value=2.4e-27 |  | 0 | 0 | no_sigp
[NAME] | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | lcl|consensus Probab=96.77  E-value=2.7e-05. PRK10696 tRNA 2-thiocytidine biosynthesis protein TtcA; Provisional Probab=96.04  E-value=0.00036 |  | 0 | 0 | no_sigp
orf_956 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff < 0.01 |  | 0 | 0 | no_sigp | Yes
orf_979 | Blast hits to [NAME], Microsporidia, Intramacronucleata, Chordata, Basidiomycota, Cnidaria, Platyhelminthes | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | HHPred web server: hit PTHR14856-unknown function (evalue = 0), PQ loop repeat family protein (evalue = 3.9e-19) | yes | 4 | 6 | 16-27
orf_1991 | Blast hits to [NAME], Microsporidia, Chordata, [NAME] | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | d2vgla_ a.118.1.10 (A:) Adaptin alpha C subunit N-terminal fragment [NAME] (Mus musculus)  Probab=99.10  E-value=8.4e-14. 2vgl_A Adaptor protein complex AP-2, alpha 2 subunit; cytoplasmic vesicle, alternative splicing, endocytosis, lipid-binding, golgi apparatus, adaptor, membrane, transport; HET: IHP; 2.59A {Rattus norvegicus}  Probab=98.73  E-value=1.7e-11 |  | 0 | 0 | no_sigp
orf_950 | Blast hits to Microsporidia, Chordata, Actinobacteria | NOT in KOG |  | orf_950 | 1 | 1 | 2 | 1 | 1 | [NAME]_ [NAME] (A:) Rac-alpha serine/threonine kinase {Human (Homo sapiens) [TaxId: 9606]} Probab=98.82  E-value=5.3e-12. 2d9y_A Pleckstrin homology domain-containing protein family A member 6; PH domain, PEPP-3, structural genomics, NPPSFA; NMR {Homo sapiens} Probab=98.74  E-value=1.6e-11 |  | 0 | 0 | no_sigp
orf_428 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | d1tbga_ b.69.4.1 (A:) beta1-subunit of the signal-transducing G protein heterotrimer {Cow (Bos taurus) [TaxId: 9913]} Probab=95.45  E-value=0.0016 |  | 0 | 0 | no_sigp
orf_1904 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | 3ott_A Two-component system sensor histidine kinase; beta-propeller, beta-sandwich, transcription; HET: TBR;   2.30A {Bacteroides thetaiotaomicron}   Probab=97.05  E-value=0.0028 |  | 0 | 0 | no_sigp
orf_3267 | Blast hits to  Spirochaetes, Microsporidia, Ascomycota, Actinobacteria, Basidiomycota, [NAME], Chlamydiae/Verrucomicrobia group, Platyhelminthes, Proteobacteria, Bacillariophyta | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | d1fx2a_ d.58.29.1 (A:) Receptor-type monomeric adenylyl cyclase {Trypanosome (Trypanosoma brucei), different isoform [TaxId: 5691]} Probab=98.85  E-value=3.7e-12 |  | 0 | 1 | 17-64
orf_1000 | Blast hits to  Spirochaetes, Bacteroidetes/Chlorobi group, Microsporidia, Deferribacteres, Firmicutes, Nitrospirae, Chlamydiae/Verrucomicrobia group, Synergistetes, Planctomycetes, Actinobacteria, Cyanobacteria, Fibrobacteres/Acidobacteria group, Thermotogae, Aquificae, Fusobacteria, Proteobacteria, [NAME] | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | COG0283 [NAME] kinase [Nucleotide transport and metabolism] Probab=100.00  E-value=0. PRK12269 bifunctional cytidylate kinase/ribosomal protein S1; Provisional Probab=100.00  E-value=0 |  | 0 | 0 | no_sigp
orf_230 | Blast hits to [NAME], Microsporidia, Intramacronucleata, Ascomycota, Basidiomycota, [NAME], | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | COG0170 SEC59 Dolichol kinase [Lipid metabolism] Probab=98.99  E-value=5.1e-13. PF01148 CTP_transf_1:  Cytidylyltransferase family;  InterPro: [NAME] cytidylyltransferase (2.7.7.41 from EC (also known as CDP- diacylglycerol synthase) (CDS). Probab=96.31  E-value=0.00016 |  | 2 | 7 | 20-30
orf_1395 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | [NAME] Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like.  Probab=96.85  E-value=1.9e-05 |  | 0 | 1 | no_sigp
orf_3041 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_3041 | 1 | 1 | 2 | 1 | 1 | cd07308 lectin_leg-like legume-like lectins: ERGIC-53, ERGL, VIP36, VIPL, EMP46, and EMP47. The legume-like (leg-like) lectins are eukaryotic intracellular sugar transport proteins with a carbohydrate recognition domain similar to that of the legume lectins.   Probab=98.55  E-value=1.3e-10 |  | 1 | 1 | 21-22
orf_1753 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1753 | 1 | 0 | 1 | 1 | 1 | cd05831 Ribosomal_P1 Ribosomal protein P1. This subfamily represents the eukaryotic large ribosomal protein P1.  Probab=98.58  E-value=9.4e-11 |  | 0 | 0 | 23-23
orf_2652 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | 3a1j_A Cell cycle checkpoint control protein RAD9A; DNA damage, DNA repair, exonuclease, hydrolase, nuclease,   nucleus, phosphoprotein, polymorphism, cytoplasm; HET: SUC; 2.50A {Homo sapiens} PDB:  3ggr _A (evalue 0.0096) |  | 0 | 0 | 23-23
orf_2911 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2911 | 1 | 1 | 0 | 1 | 1 | Peregrin; four-helix bundle, transcription activator, structural genomics, NPPSFA, national project on   protein structural and functional analyses; NMR {Homo sapiens} (evalue=0.0017), second best hit: Bromodomain-containing protein 7; helix, LEFT-handed four-helix bundle, transcription; NMR {Homo sapiens} (evalue=0.0058) |  | 0 | 1 | 21-21
orf_2335 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_2335 | 1 | 0 | 0 | 1 | 1 | 3q87_A Putative uncharacterized protein ECU08_1170; SAM-methyltransferase, methyltransferase, methylation, trans activator-transferase complex; HET: SAM; 2.00A {Encephalitozoon cuniculi} Probab=100.00  E-value=8.9e-36 |  | 0 | 0 | no_sigp
orf_601 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | 3pev_B Nucleoporin GLE1; RECA, heat, DEAD-BOX, ATPase, helicase, mRNA export, nuclear hydrolase; HET: IHP; 2.50A {Saccharomyces cerevisiae} PDB: 3peu_B* 3pex_B* 3pez_B* Probab=98.45  E-value=3.7e-10 |  | 0 | 0 | no_sigp
orf_146 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 0 | 0 | 3oym_A PFV integrase, P42IN; protein-DNA complex; HET: DNA; 2.02A {Human spumaretrovirus} PDB (Probab=95.89  E-value=0.00055) |  | 0 | 0 | no_sigp
orf_2556 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 1 | 13 | 1 | 1 | 3n4x_A Monopolin complex subunit CSM1; meiosis, rDNA, replication (Probab=94.08  E-value=0.012) |  | 0 | 0 | no_sigp
orf_2984 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | 1oqy_A HHR23A, UV excision repair protein RAD23 homolog A; DNA repair, proteasome-mediated degradation, protein-protein interaction, replication; NMR {Homo sapiens}    Probab=97.13  E-value=0.0016 |  | 1 | 2 | no_sigp
orf_3278 | Blast hits to Microsporidia, Cnidaria | NOT in KOG |  | orf_3278 | 1 | 1 | 0 | 1 | 1 | 3m1i_B RAN-specific GTPase-activating protein 1; heat repeat, GTP-binding, nucleotide-binding, NUCL protein transport, transport, cytoplasm, GTPase activation; HET: GTP; (Probab=98.12  E-value=7.5e-09) |  | 0 | 0 | no_sigp
orf_1130 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1130 | 1 | 1 | 1 | 1 | 1 | 3l1w_A Uncharacterized protein; APC29019.2, conserved protein, enterococcus faecalis V583, [NAME], MCSG, structural genomics; 1.60A {Enterococcus faecalis} Probab=98.75  E-value=1.4e-11. 3g6s_A Putative endonuclease/exonuclease/phosphatase family protein; alpha-beta protein, structural genomics, PSI-2; 2.50A {Bacteroides vulgatus atcc 8482} Probab=98.74  E-value=1.6e-11 |  | 0 | 0 | 14-22
orf_385 | Blast hits to Firmicutes, Microsporidia | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | 3k49_A MRNA-binding protein PUF3; [NAME], pumilio, RNA binding, mitochondrial mRNA,, cytoplasm, membrane, mitochondrion; HET: CIT; 2.50A {Saccharomyces cerevisiae} PDB: 3k4e_A Probab=98.96  E-value=8e-13 |  | 0 | 0 | no_sigp
orf_1431 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | 3hxj_A Pyrrolo-quinoline quinone; all beta protein. incomplete 8-blade beta-propeller., structural genomics, PSI-2; 2.00A {Methanococcus maripaludis C7} Probab=95.76  E-value=0.00077 |  | 0 | 0 | no_sigp
orf_1547 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1547 | 1 | 4 | 0 | 1 | 1 | 3hbe_X Class IV chitinase CHIA4-PA2; endochitinase, family 19, conformational changes, chitin-binding, glycosidase, hydrolase; 1.55A {Picea abies} PDB: (Probab=98.00  E-value=2.1e-08 ). |  | 0 | 0 | 16-23
orf_2288, orf_2675, orf_2837 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 3 | 1 | 0 | 0 | 0 | 3g91_A MTH0212, exodeoxyribonuclease; double-strand specific 3'-5' exonuclease, AP endonuclease; HET: PG4; 1.23A Probab=97.53  E-value=5e-07 |  | 0 | 0 | no_sigp
orf_565 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | 3ei3_A DNA damage-binding protein 1; UV-damage, DDB, nucleotide excision repair, xeroderma pigmentosum, cytoplasm, DNA repair; HET: DNA PG4; 2.30A {Homo sapiens}  Probab=99.96  E-value=4e-34 |  | 0 | 0 | no_sigp
orf_1781 | Blast hits to Microsporidia, [NAME] | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | 3dtp_E RLC, [NAME] light chain; muscle protein, smooth muscle, [NAME] subfragment 2, heavy meromyosin, essential light chain; 20.00A {Avicularia avicularia} (Probab=96.73  E-value=3.2e-05 ). d1hqva_ a.39.1.8 (A:) Apoptosis-linked protein alg-2 {[NAME] (Mus musculus) (Probab=96.48  E-value=8.6e-05 ) |  | 0 | 0 | no_sigp
orf_1359 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 0 | 0 | No hit at cutoff < 0.01 |  | 0 | 0 | no_sigp | Yes
orf_1355 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | 2ziu_B Crossover junction endonuclease EME1; helix-hairpin-helix, alternative splicing, DNA damage, DNA recombination, DNA repair, hydrolase, magnesium; 2.70A {Homo sapiens} PDB: 2ziw_B 2zix_B 2ziv_B Probab=97.53  E-value=5.3e-07 |  | 0 | 0 | no_sigp
orf_1023 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1023 | 1 | 1 | 0 | 1 | 1 | 2y6e_A Ubiquitin carboxyl-terminal hydrolase 4; HET: CME; 2.40A {Homo sapiens} Probab=98.31  E-value=1.4e-09 |  | 0 | 0 | no_sigp
orf_1488 | Blast hits to Microsporidia, Ascomycota, Chordata, Cnidaria | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | 2xub_A DNA-directed RNA polymerase III subunit RPC3; transcription, winged helix; 2.80A {Homo sapiens} PDB: 2xv4_S Probab=99.87  E-value=1.3e-26 |  | 0 | 0 | no_sigp
orf_2880 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 1 | 1 | 1 | 2wa0_A Melanoma-associated antigen 4; immune system, multiple anomalous dispersion, cancer/testis antigen 1.4, melanoma antigen family A 4, CT1.4, MAGE-41; 2.30A {Homo sapiens} Probab=97.21  E-value=3.3e-06. PF01454 MAGE:  MAGE family; |  | 0 | 1 | no_sigp
orf_1730 | Blast hits to Bacteroidetes/Chlorobi group, Microsporidia, Firmicutes, [NAME], Ascomycota, Actinobacteria, Cyanobacteria, Fibrobacteres/Acidobacteria group, Thermotogae, Basidiomycota, Chlamydiae/Verrucomicrobia group, Fusobacteria, Planctomycetes, Proteobacteria, Dictyoglomi, Euryarchaeota | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | 2vyo_A ECU11_0510, chitooligosaccharide deacetylase; CE4 esterase, native protein, microsporidian, chitin deacetylase, hydrolase, inactive; 1.50A {Encephalitozoon cuniculi} Probab=100.00  E-value=0 |  | 0 | 0 | 17-32
orf_1123 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_1123 | 1 | 0 | 1 | 1 | 1 | 2pi2_E Replication protein A 14 kDa subunit; FULL-length RPA14/32, ssDNA binding protein, OB-fold, dioxane, DNA binding protein; 2.00A {Homo sapiens} SCOP: [NAME]: 2pqa_B 2z6k_C Probab=97.98  E-value=2.3e-08  Score=69.52 |  | 0 | 0 | no_sigp
orf_1592 | Blast hits to Microsporidia, Euryarchaeota, Thaumarchaeota | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | [NAME]; polyadenylation, metallo-B-lactamase, PRE-mRNA processing, artemis, V(D)J recombination, double-strand break repair (Probab=99.51  E-value=8e-18 ) |  | 0 | 0 | no_sigp
orf_743 | Blast hits to Spirochaetes, Cyanobacteria, Microsporidia, Intramacronucleata, Chlamydiae/Verrucomicrobia group, [NAME], Ascomycota, Chordata, Bacteroidetes/Chlorobi group, Basidiomycota, Cnidaria, Proteobacteria, [NAME], Euryarchaeota, [NAME] | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | 2ctq_A DNAJ homolog subfamily C member 12; J-domain, chaperone, helix-turn-helix, structural genomics, NPPSFA; NMR {Homo sapiens} (Probab=97.25  E-value=2.7e-06) |  | 0 | 0 | no_sigp
orf_1447 | Blast hits to Microsporidia, Ascomycota | NOT in KOG |  |  | 1 | 1 | 0 | 0 | 0 | 2cay_A VPS36 PH domain, vacuolar protein sorting protein 36; transport, ESCRT-II, lipid-binding, multivesicular bodies, membrane; 1.9A {Saccharomyces cerevisiae} SCOP: b.55.1.12 (Probab=96.26  E-value=0.00018) |  | 0 | 0 | no_sigp
orf_2259 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | 1z7x_W Ribonuclease inhibitor; leucine-rich repeat, enzyme- inhibitor complex, structural genomics, protein structure initiative, PSI, CESG; HET: CIT; 1.95A {Homo sapiens}  Probab=94.16  E-value=0.01 |  | 0 | 0 | no_sigp
orf_1978 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex, and is degraded during the mitotic phase of the cell cycle. It has been proposed that geminin inhibits DNA replication during S, G2, and M phases and that geminin destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.                          Probab=96.74  E-value=0.0039 |  | 0 | 0 | no_sigp
orf_172 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | 1a79_A TRNA endonuclease; 2.28A {[NAME] jannaschii} SCOP: c.52.2.1 d.75.1.1 Probab=96.52  E-value=7.3e-05 |  | 0 | 1 | no_sigp
orf_28 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | d1nr0a1 b.69.4.1 (A:2-312) Actin interacting protein 1 {Nematode (Caenorhabditis elegans) [TaxId: 6239]}   Probab=100.00  E-value=3.1e-30 |  | 0 | 0 | no_sigp
orf_1909 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 1 | 0 | 1 | 1 | No hit at cutoff < 0.01 |  | 0 | 0 | no_sigp | Yes
orf_2137 | Blast hits to Spirochaetes, Bacteroidetes/Chlorobi group, Microsporidia, [NAME], Firmicutes, Actinobacteria, [NAME], Fusobacteria, Proteobacteria, Bacillariophyta | NOT in KOG |  |  | 1 | 1 | 45 | 0 | 0 | d12asa_ d.104.1.1 (A:) Asparagine synthetase {Escherichia coli [TaxId: 562]} Probab=100.00  E-value=0 |  | 0 | 0 | no_sigp
orf_1674 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 1 | 1 | 1 | cd05791 S1_CSL4 S1_CSL4: CSL4, S1-like RNA-binding domain. S1-like RNA-binding domains are found in a wide variety of RNA-associated proteins. ScCSL4 protein is a subunit of the exosome complex. The exosome plays a central role in 3' to 5' RNA processing and degradation in eukarytes and archaea. Its functions include the removal of incorrectly processed RNA and the maintenance of proper levels of mRNA, rRNA and a number of small RNA species. In S. cerevisiae, the exosome includes nine core components, six of which are homologous to bacterial RNase PH. These form a hexameric ring structure. The other three subunits (RrP4, Rrp40, and Csl4) contain an S1 RNA binding domain and are part of the "S1 pore structure". Probab=98.09  E-value=9.7e-09 |  | 0 | 0 | no_sigp
orf_3037 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_3037 | 1 | 0 | 1 | 1 | 1 | PF00789 UBX:  UBX domain;  InterPro: The UBX domain is found in ubiquitin-regulatory proteins, which are members of the ubiquitination pathway, as well as a number of other proteins including FAF-1 (FAS-associated   factor 1), the human Rep-8 reproduction protein and several hypothetical proteins from yeast. The function of   the UBX domain is not known although the fragment of avian FAF-1 containing the UBX domain causes apoptosis of  transfected cells. Probab=97.32  E-value=0.0046 |  | 0 | 1 | 24-24
orf_543 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff < 0.01 |  | 2 | 2 | 29-32 | Yes
orf_2168 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | TIGR03304 (E-value=2.7e-15)outer membrane insertion C-terminal signal. TIGR01167 (E-value=1.1e-11) LPXTG-motif cell wall anchor domain. TIGR03057 (E-value=8.2e-08) |  | 2 | 4 | 25-27
orf_1451 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | TIGR03304 (Probab=99.45  E-value=6.2e-17) outer membrane insertion C-terminal signal. TIGR01167 (Probab=99.09  E-value=1.4e-13) LPXTG-motif cell wall anchor domain. TIGR03057 (Probab=97.53  E-value=7.2e-07) X-X-X-Leu-X-X-Gly heptad repeats. TIGR00756 (Probab=95.53  E-value=0.0019) pentatricopeptide repeat domain |  | 1 | 2 | no_sigp
orf_3050 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 1 | 1 | No hit at cutoff < 0.01 |  | 0 | 1 | 17-24 | Yes
orf_1776 | Blast hits to Microsporidia, Actinobacteria | NOT in KOG |  | orf_1776 | 1 | 0 | 0 | 1 | 1 | TIGR01167 (E-value=7.9e-17) LPXTG-motif cell wall anchor domain. TIGR03304 (E-value=4.9e-14) outer membrane insertion C-terminal signal. TIGR03057 (E-value=6.6e-08) X-X-X-Leu-X-X-Gly heptad repeats. TIGR00756 (Probab=94.33  E-value=0.013) pentatricopeptide. PIRSF032522 (Probab=93.58  [NAME]) TcaA repeat domain; |  | 1 | 1 | no_sigp
orf_981 | Within-Microsporidia Blast hits only | NOT in KOG |  |  | 1 | 0 | 0 | 0 | 1 | PF10197 Cir_N:  N-terminal domain of CBF1 interacting co-repressor CIR;  InterPro:  This entry represents a 45 residue conserved region at the N-terminal end of a family of proteins referred to as CIRs (CBF1-interacting co-repressors).  Probab=99.49  E-value=1e-13 |  | 0 | 0 | no_sigp
orf_777 | Within-Microsporidia Blast hits only | NOT in KOG |  | orf_777 | 1 | 1 | 1 | 1 | 1 | No hit at cutoff < 0.01 |  | 0 | 0 | no_sigp | Yes