Spreadsheet: 37fb5ab9-6458-4b43-9429-2d4e28e59313
Sheet: Sheet1

ChIP-qPCR Data Analysis for % Input and Fold Enrichment Calculations | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | SIGMA | Unnamed: 11
Enter the raw Ct values from the RNAP ChIP-qPCR data for [NAME] and ZNF333-3' primers in the grey cells
Enter the raw Ct values from your antibody ChIP-qPCR data for target and non-target primers in the blue cells
Results for RNAP ChIP will be calculated and recorded as % Input values in green and Fold Enrichment values in yellow
Results for your Ab ChIP will be calculated and recorded as [NAME] values in pink and Fold Enrichment values in orange
The default Input fraction is 1% which is a dilution factor (DF) of 100 or 6.644 cycles (i.e. log2 of 100), NS=Non-Specific Ab (Mu/Rb IgG)
Positive Control RNAP ChIP
 | Target | Input normalized | Yield | Control adjusted Ct | Fold |  | Inter-genic | Input normalized | Yield | Control adjusted Ct | Fold
 | Promoter | Δ Ct | % In | DDCt | Enrichment |  | region | Δ Ct | % In | DDCt | Enrichment
Antibody | [NAME] | (Ct[IP]-Ct[InputxDF]) | ((2-∆Ct)x100%) | (∆Ct[IP]-∆Ct[NS]) | (2-∆∆Ct) |  | ZNF333-3' | (Ct[IP]-Ct[InputxDF]) | ((2-∆Ct)x100%) | (∆Ct[IP]-∆Ct[NS]) | (2-∆∆Ct)
[NAME] |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
RNAP |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
1 % Input |  | 6.644 | 0.9999003233158655 | 0 |  |  |  | 6.644 | 0.9999003233158655 | 0
Your Antibody 1* ChIP
 | Target* | Input normalized | Yield | Control adjusted Ct | Fold |  | Non-target* | Input normalized | Yield | Control adjusted Ct | Fold
Antibody | Promoter | DCt | % In | DDCt | Enrichment |  | Promoter | DCt | % In | DDCt | Enrichment
NS* IgG |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
Your* Ab |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
1 % Input |  | 6.644 | 0.9999003233158655 | 0 |  |  |  | 6.644 | 0.9999003233158655 | 0
* Customize these fields with your ChIP specific information
Your Antibody 2* ChIP
 | Target* | Input normalized | Yield | Control adjusted Ct | Fold |  | Non-target* | Input normalized | Yield | Control adjusted Ct | Fold
Antibody | Promoter | DCt | % In | DDCt | Enrichment |  | Promoter | DCt | % In | DDCt | Enrichment
NS* IgG |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
Your* Ab |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
1 % Input |  | 6.644 | 0.9999003233158655 | 0 |  |  |  | 6.644 | 0.9999003233158655 | 0
* Customize these fields with your ChIP specific information
Your Antibody 3* ChIP
 | Target* | Input normalized | Yield | Control adjusted Ct | Fold |  | Non-target* | Input normalized | Yield | Control adjusted Ct | Fold
Antibody | Promoter | DCt | % In | DDCt | Enrichment |  | Promoter | DCt | % In | DDCt | Enrichment
NS* IgG |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
Your* Ab |  | 6.644 | 0.9999003233158655 | 0 | 1 |  |  | 6.644 | 0.9999003233158655 | 0 | 1
1 % Input |  | 6.644 | 0.9999003233158655 | 0 |  |  |  | 6.644 | 0.9999003233158655 | 0
* Customize these fields with your ChIP specific information