Spreadsheet: 268726d1380756613-help-circumference-error-circumference-error
Sheet: Table 7 proc stab(% and MFI)

Time=0
 | Relative Percentage
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 54.9 | 11 | 22.8 | 29.2 | 18.2 | 93.4
MF39735M | 61.1 | 9.4 | 17.9 | 64.1 | 18.3 | 97.4
MF39746M | 61.4 | 5.6 | 15.9 | 34.6 | 13.5 | 89.8
MF39758F | 70 | 14.9 | 13.8 | 55.8 | 13.6 | 95.4
MF39755F | 56.7 | 28.2 | 17.4 | 38.5 | 24.5 | 94.9
MF39754F | 64.8 | 22.7 | 23.2 | 67.9 | 8.1 | 94.8
MF39756F | 61.6 | 7.9 | 26.5 | 34.7 | 6.6 | 78.1
MF39757F | 65.8 | 21 | 21.6 | 44.7 | 10.2 | 87.5
MF39743M | 72 | 3.9 | 10.9 | 35.8 | 9.8 | 91.8
MF39720M | 68 | 9.1 | 23.8 | 29.9 | 4.3 | 90.8
Mean | 63.6 | 13.4 | 19.4 | 43.5 | 12.7 | 91.4
SD | 5.5 | 8.1 | 5 | 14.2 | 6.2 | 5.5
Time=24
 | Relative Percentage
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 53.7 | 4.4 | 24.6 | 7.7 | 14.8 | 81.4
MF39735M | 58.9 | 2.5 | 18.9 | 38.8 | 17.8 | 97
MF39746M | 67.4 | 3.6 | 16.5 | 27.4 | 12.9 | 82.6
MF39758F | 68.8 | 7.6 | 14.2 | 24.8 | 12.3 | 91
MF39755F | 56 | 14.1 | 17.6 | 15.9 | 24 | 88.7
MF39754F | 63 | 16.9 | 24.5 | 47.9 | 7.4 | 92.7
MF39756F | 60.1 | 5.7 | 27.4 | 27 | 7.2 | 71.2
MF39757F | 64.5 | 9 | 23 | 24.7 | 11.1 | 76.9
MF39743M | 70.5 | 2 | 11.5 | 28.4 | 14.1 | 87.3
MF39720M | 69 | 5.7 | 23.5 | 21.2 | 5.2 | 82.3
Mean | 63.2 | 7.2 | 20.2 | 26.4 | 12.7 | 85.1
SD | 5.8 | 4.9 | 5.2 | 11.1 | 5.6 | 7.7
Time=48
 | Relative Percentage
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 54.8 | 4.4 | 22.7 | 8.9 | 17.2 | 81.3
MF39735M | 59.3 | 2.5 | 18.7 | 42.5 | 18.9 | 96.6
MF39746M | 68.2 | 4.4 | 15.8 | 30.3 | 13 | 85.1
MF39758F | 67.5 | 8.3 | 14.8 | 25.1 | 13.1 | 91.8
MF39755F | 56 | 14.4 | 17.9 | 14.9 | 24 | 88.5
MF39754F | 61.9 | 19.6 | 24.6 | 53.7 | 8.3 | 93.1
MF39756F | 61.1 | 7.5 | 26.5 | 30 | 7.7 | 72.1
MF39757F | 63.5 | 10.1 | 22.4 | 26 | 12.2 | 78.7
MF39743M | 70.4 | 2.6 | 11.7 | 28.4 | 15.1 | 89.4
MF39720M | 66.7 | 6.5 | 25.3 | 25.7 | 6.1 | 79
Mean | 62.9 | 8 | 20 | 28.6 | 13.6 | 85.6
SD | 5.3 | 5.5 | 5 | 12.6 | 5.5 | 7.7
 | SUMMARY
 | Mean (Relative Percentage)
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
0 hr | 63.6 | 13.4 | 19.4 | 43.5 | 12.7 | 91.4
24 hr | 63.2 | 7.2 | 20.2 | 26.4 | 12.7 | 85.1
48 hr | 62.9 | 8 | 20 | 28.6 | 13.6 | 85.6
 | % Difference
0 vs 24 hr | 0.6 | 60.2 | 4 | 48.9 | 0 | 7.1
Pass/Fail | Pass | Fail | Pass | Fail | Pass | Pass
 | % Difference
0 vs 48 hr | 1.1 | 50.5 | 3 | 41.3 | 6.8 | 6.6
Pass/Fail | Pass | Fail | Pass | Fail | Pass | Pass
Time=0
 | Mean Fluorescence Intensity of AF488
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 352 | 1427 | 416 | 940 | 1977 | 2093
MF39735M | 346 | 1404 | 1088 | 1560 | 5190 | 5320
MF39746M | 253 | 1286 | 535 | 1204 | 4172 | 4617
MF39758F | 438 | 1457 | 850 | 1308 | 3366 | 3517
MF39755F | 588 | 1342 | 613 | 1181 | 2607 | 2731
MF39754F | 548 | 1485 | 1253 | 1580 | 3915 | 4111
MF39756F | 267 | 1157 | 645 | 1233 | 1804 | 2241
MF39757F | 644 | 1661 | 991 | 1634 | 4909 | 5596
MF39743M | 282 | 2168 | 738 | 1430 | 3840 | 4164
MF39720M | 342 | 1454 | 631 | 1360 | 2817 | 3058
Mean | 406 | 1484 | 776 | 1343 | 3460 | 3745
SD | 141 | 274 | 265 | 215 | 1157 | 1226
Time=24
 | Mean Fluorescence Intensity of AF488
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 318 | 1517 | 358 | 1188 | 1377 | 1579
MF39735M | 308 | 1532 | 710 | 1268 | 3524 | 3623
MF39746M | 266 | 1077 | 469 | 1041 | 2829 | 3360
MF39758F | 348 | 1245 | 595 | 1288 | 2330 | 2531
MF39755F | 455 | 1193 | 508 | 1411 | 1885 | 2069
MF39754F | 425 | 1102 | 809 | 1310 | 2677 | 2861
MF39756F | 271 | 935 | 537 | 1048 | 1208 | 1580
MF39757F | 457 | 1399 | 641 | 1267 | 2936 | 3754
MF39743M | 280 | 1738 | 612 | 1197 | 2358 | 2655
MF39720M | 318 | 1180 | 528 | 1148 | 1876 | 2177
Mean | 345 | 1292 | 577 | 1217 | 2300 | 2619
SD | 74 | 248 | 127 | 116 | 725 | 787
Time=48
 | Mean Fluorescence Intensity of AF488
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 341 | 1248 | 397 | 1069 | 1307 | 1484
MF39735M | 346 | 1562 | 717 | 1167 | 3064 | 3158
MF39746M | 313 | 994 | 492 | 974 | 2662 | 3067
MF39758F | 383 | 1144 | 634 | 1359 | 2167 | 2327
MF39755F | 474 | 1130 | 509 | 1300 | 1747 | 1915
MF39754F | 471 | 1111 | 839 | 1232 | 2470 | 2624
MF39756F | 316 | 862 | 583 | 1030 | 1195 | 1525
MF39757F | 497 | 1363 | 668 | 1269 | 2746 | 3416
MF39743M | 337 | 1541 | 631 | 1192 | 2194 | 2408
MF39720M | 364 | 1102 | 578 | 1112 | 1721 | 2033
Mean | 384 | 1206 | 605 | 1170 | 2127 | 2396
SD | 70 | 226 | 124 | 124 | 626 | 673
 | SUMMARY
 | Mean Fluorescence Intensity of AF488
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
0 hr | 406 | 1484 | 776 | 1343 | 3460 | 3745
24 hr | 345 | 1292 | 577 | 1217 | 2300 | 2619
48 hr | 384 | 1206 | 605 | 1170 | 2127 | 2396
 | % Difference
0 vs 24 hr | 16 | 14 | 29 | 10 | 40 | 35
Pass/Fail | Pass | Pass | Pass | Pass | Fail | Fail
 | % Difference
0 vs 48 hr | 6 | 21 | 25 | 14 | 48 | 44
Pass/Fail | Pass | Pass | Pass | Pass | Fail | Fail
Time=0
 | Absolute Count
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 2260.233 | 249 | 938.676 | 274 | 749.294 | 700
MF39735M | 5512.442 | 518 | 1614.938 | 1035 | 1651.026 | 1608
MF39746M | 2290.834 | 128 | 593.229 | 205 | 504 | 453
MF39758F | 4534.599999999999 | 676 | 893.964 | 499 | 881.008 | 840
MF39755F | 2484.027 | 700 | 762.294 | 293 | 1073 | 1018
MF39754F | 3959.928 | 899 | 1417.752 | 963 | 494.991 | 469
MF39756F | 6280.12 | 496 | 2702 | 938 | 672.87 | 526
MF39757F | 3887.463999999999 | 816 | 1276.128 | 570 | 603 | 528
MF39743M | 4967.28 | 194 | 751.991 | 269 | 676.102 | 621
MF39720M | 3972.56 | 362 | 1390.396 | 416 | 251.206 | 228
Mean | 4015 | 504 | 1234 | 546 | 756 | 699
SD | 1373 | 268 | 617 | 319 | 386 | 387
Time=24
 | Absolute Count
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 2210.829 | 97 | 1012.782 | 78 | 609.316 | 496
MF39735M | 5313.958 | 133 | 1705.158 | 662 | 1605.916 | 1558
MF39746M | 2514.694 | 91 | 615.615 | 169 | 481.299 | 398
MF39758F | 4456.864 | 339 | 919.8759999999999 | 228 | 796.7940000000001 | 725
MF39755F | 2453.36 | 346 | 771.056 | 123 | 1051.44 | 933
MF39754F | 3849.93 | 651 | 1497.195 | 717 | 452.2140000000001 | 419
MF39756F | 6127.195 | 349 | 2793.43 | 754 | 734.0400000000001 | 523
MF39757F | 3810.66 | 343 | 1358.84 | 336 | 655.788 | 504
MF39743M | 4863.795 | 97 | 793.385 | 225 | 972.7589999999999 | 849
MF39720M | 4030.98 | 230 | 1372.87 | 291 | 303.784 | 250
Mean | 3963 | 268 | 1284 | 358 | 766 | 666
SD | 1293 | 176 | 639 | 255 | 374 | 378
Time=48
 | Absolute Count
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 2256 | 99 | 934.559 | 83 | 708.1239999999999 | 576
MF39735M | 5350 | 134 | 1687.114 | 717 | 1705.158 | 1647
MF39746M | 2545 | 112 | 589.498 | 179 | 485.03 | 413
MF39758F | 4373 | 363 | 958.7440000000001 | 241 | 848.618 | 779
MF39755F | 2453 | 353 | 784.199 | 117 | 1051.44 | 931
MF39754F | 3783 | 741 | 1503.306 | 807 | 507.213 | 472
MF39756F | 6229 | 467 | 2701.675 | 811 | 785.015 | 566
MF39757F | 3752 | 379 | 1323.392 | 344 | 720.776 | 567
MF39743M | 4857 | 126 | 807.183 | 229 | 1041.749 | 931
MF39720M | 3897 | 253 | 1478.026 | 380 | 356.362 | 282
Mean | 3950 | 303 | 1277 | 391 | 821 | 716
SD | 1304 | 203 | 619 | 283 | 385 | 390
 | SUMMARY
 | Mean (Absolute Count)
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
0 hr | 4015 | 504 | 1234 | 546 | 756 | 699
24 hr | 3963 | 268 | 1284 | 358 | 766 | 666
48 hr | 3950 | 303 | 1277 | 391 | 821 | 716
 | % Difference
0 vs 24 hr | 1 | 61 | 4 | 42 | 1 | 5
Pass/Fail | Pass | Fail | Pass | Fail | Pass | Pass
 | % Difference
0 vs 48 hr | 2 | 50 | 3 | 33 | 8 | 2
Pass/Fail | Pass | Fail | Pass | Fail | Pass | Pass
 | Calculations for Lymphocyte Count
Animal | # of Events R1 |  | # of Events R2 |  | # of Beads Per Test | Test Volume | Total
MF39741M | 7168 |  | 1762 |  | 50602 | 50 | 4117
MF39735M | 8032 |  | 901 |  | 50602 | 50 | 9022
MF39746M | 6871 |  | 1864 |  | 50602 | 50 | 3731
MF39758F | 7969 |  | 1245 |  | 50602 | 50 | 6478
MF39755F | 7437 |  | 1718 |  | 50602 | 50 | 4381
MF39754F | 7808 |  | 1293 |  | 50602 | 50 | 6111
MF39756F | 8341 |  | 828 |  | 50602 | 50 | 10195
MF39757F | 7682 |  | 1316 |  | 50602 | 50 | 5908
MF39743M | 7846 |  | 1151 |  | 50602 | 50 | 6899
MF39720M | 7868 |  | 1363 |  | 50602 | 50 | 5842