Spreadsheet: bd1c482a-951e-448e-adfd-d33c2095a895
Sheet: Sheet1

Primer Name | Sequence | Function
Avh_307-SPF-BamHI | atGGATCCATGGAGGCTGCTGAATCGGCTG | Avr3b P6497 and P7076 allele construct for soybean cobombardment (without signal peptide)
Avh_307R-SphI | atGCATGCCTAATTAAACAACAATCGTACTG | Avr3b P6497 allele construct for soybean cobombardment
Avh_307R2-SphI | atGCATGCTTACCGTACCCCTCCTTCTC | Avr3b P7076 allele construct for soybean cobombardment
pFF19-3bNdx-F | ctaggatccATGGGTCGTAGCGTGGTATGCAATG | Avr3b deletion mutant (16-174 a.a.) for soybean cobombardment
pFF19-3bW-R | ctagcatgcCTATACCACGCTACGACCG | Avr3b deletion mutant (170-315 a.a.) for soybean cobombardment
Avh_307RTF | [NAME] | Avr3b transcriptional profiling for P6497 (Real Time RT-PCR)
Avh_307RTR | CTAATTAAACAACAATCGTACTG | Avr3b transcriptional profiling for P6497 (Real Time RT-PCR)
Avh307QRT-F3 | GGTCGTAGCGTGGTATGCAATG | Avr3b transcriptional polymorphism for P6497 and P7076 (Real Time RT-PCR)
Avh307QRT-R3 | TTCTTCCAGGACCTCACGCTTC | Avr3b transcriptional polymorphism for P6497 and P7076 (Real Time RT-PCR)
Avh307MF | CTGTTTCGACATCGCGAAGC | Avr3b Polymorphism sequencing (PCR for product)
Avh307MR | TCAAGTGAAATCTCTCTATTCC | Avr3b Polymorphism sequencing (PCR for product)
Avh307MFa | CCTAAAGGTCTCAGCGAGAA | Avr3b Polymorphism sequencing (Primers for sequencing)
Avh307MFb | CCTGCAGAGCCATCACAAG | Avr3b Polymorphism sequencing (Primers for sequencing)
PsActin1F | ACTGCACCTTCCAGACCATC | Actin for control  (Real Time RT-PCR)
PsActin1R | CCACCACCTTGATCTTCATG | Actin for control  (Real Time RT-PCR)
A3b-flag-F | gATGGATTACAAGGATGATGATGATAAGGAGGCTGCTGAATCGGCTG | Avr3b expression in PVX vector plus Flag-tag
A3b-flag-R | aCTAATTAAACAACAATCGTACTG | Avr3b expression in PVX vector
A3b-flag-16-174R | aCTATACCACGCTACGACCG | Avr3b deletion mutant (16-174 a.a.) expression in PVX vector
A3b-flag-170-315F | gATGGATTACAAGGATGATGATGATAAGGGTCGTAGCGTGGTATGCAATG | Avr3b deletion mutant (170-315 a.a.) expression in PVX vector
3bQQQQ-F | CTTATGGGGCGGCGAAGCAACAAGTCCTGCAACAAGGAGGGGTACGGTATCG | Avr3b QQQQ mutant expression in PVX vector
3bQQQQ-R | CGATACCGTACCCCTCCTTGTTGCAGGACTTGTTGCTTCGCCGCCCCATAAG | Avr3b QQQQ mutant expression in PVX vector
NbActF | ACCATCAATGATCGGAATGG | [NAME] (Real Time PCR for virulence assay)
NbActR | GCTCATCCTATCAGCAATGC | [NAME] (Real Time PCR for virulence assay)
PcActF | [NAME] | P. capsici actin amplication (Real Time PCR for virulence assay)
PcActR | CCGACTCATCATACTCGG | P. capsici actin amplication (Real Time PCR for virulence assay)
Avh309RTF | CAAAGTGGCAAGCTTCGTAG | RT-PCR to detect the presence of transcript
Avh309RTR | [NAME] | RT-PCR to detect the presence of transcript
Avh308RTF | CAAGGTGAGCGCAATAGACG | RT-PCR to detect the presence of transcript
Avh308RTR | CTAGAGCACAGCTTTCATTTG | RT-PCR to detect the presence of transcript
Avh9RTF | AGCCCACGATAACGGCGCTC | RT-PCR to detect the presence of transcript
Avh9RTR | CACAGGCTTTTTGTCGAGATG | RT-PCR to detect the presence of transcript
Avh198RTF | ATCGAGCGTTTTATTTGTGG | RT-PCR to detect the presence of transcript
Avh198RTR | TAGCCAGGGATTGCCCAGTTG | RT-PCR to detect the presence of transcript
Avh340RTF | ACGAAGAACGATGCGTCTCC | RT-PCR to detect the presence of transcript
Avh340RTR | CTTCGTAATAAGTATTGTACC | RT-PCR to detect the presence of transcript
Avh238RTF | TGCCGAGGCGAAGCTCTTGTC | RT-PCR to detect the presence of transcript
Avh238RTR | CTACACAGGCTTGGCCAACC | RT-PCR to detect the presence of transcript
Avh302RTF | ATGCCTTCGGTCCGAAGAAGC | RT-PCR to detect the presence of transcript
Avh302RTR | TCATTTGCCCTCTTCCACTG | RT-PCR to detect the presence of transcript
AVH307-T7F | GTAATACGACTCACTATAGGGGGGTACGGTATCGTTCAGACAT | P. sojae Avr3b in vitro synthesized dsRNA
AVH307-T7R | [NAME] | P. sojae Avr3b in vitro synthesized dsRNA