Spreadsheet: 400af4cd-4d8f-4388-a469-4828fc87a651
Sheet: Sheet1

Table S8. List of novel transcripts coding for ORFs with | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8
homology to annotated gene products in T. cruzi and/or L. major.
Novel transcript | Predicted ORF | ORF length | Hit | Query_seq | T. cruzi hit | L. major hit | TriTrypDB Annotation | Predicted amino acid sequence
Tb2.NT.49 | orf_1_80_349 | 90 aa | Tcruzi hypothetical protein | MGHLHQWRSRQKIGMGKGSRHCVICSNQKALIRKYELNVCRQCFRENAENIGFVKLR | Tc00.[PHONE].244 | no |  | [NAME]
Tb3.NT.22 | orf_1_160_546 | 129 aa | Lmajor hypothetical protein | VPVYALPDQPYLETTVLPLLLRGLEEVVKVRPADPLAFLAAYLLSNNPQR | no | [NAME] |  | MAALLPLVLSASLVVVAVVLSILACTVVAGSNVLPLFSLLLSFITPLPFLFFGRSESTFDDDGEINGFVVFLSGALAVSAPSLSIVLYHTGYSSLGAFLLSLGSQVTLLGAAAFLQSGERQDEEGNYDL
Tb3.NT.43 | orf_1_40_1488 | 483 aa | Tcruzi, Lmajor hypothetical protein | LFNESLRTGVVPPAWKTGVIIPI---LKAGKKAEDLDSYRPVTLTSCLCKVMERIIAARLRDTVESQLTPQQSGFRPGCSTLEQLLHVRAALCRPTHQSRTGAVFVDYEKAFDTVDHDKIAREMHRMKVSPHIVKWCVSFLSNRTGRVRFKEKHSRSRTFERGVPQGTVLGPI | Tc00.[PHONE].118 | LmjF29.0870 | Tb927.3.3460 | MFCVSAARLTALASHQPILPPSVSTRVAVMRFLGDGRSRMPNINTINTLITKTGAKGLAGNASVLGKAMFYLKRGNPVGAIAEIDKILPEISDDALKRFAVGIRLRARNDLLDMKESALKVHGGITELEVSDIRTALRDDYKVLEAASPGCWLFELAVAEYRLYEGCAKDAYDSFRLVEKSIQEYILSLKKPSPSQALHGTDNHASLVLAFQLSRARTPNVPKVSSKIVDEAIKGIVEGYSTKEATLAFKKDLGAALTDEEVVELVYIFEAAHIRHHFHDYFPLHEDYRGWGSERANAAKGTLASHSTSSVFLDDKLLASIRTSVPHQPFYAGEDELRRFMSSDHKDLIAELRHTFGSGPKTSLAKSEDAYFDALKAVENSPMSADAAGSAAYPVSQNDICLCLAQQLLYRTRVNIAVALTQLDRLEEAVPILDGVINADEYIYMWRALLARGEANKRFGHIGKSDEDFRQLLQLKRITSADA
Tb5.NT.36 | orf_1_207_506 | 100 aa | Lmajor hypothetical protein | [NAME] | no | LmjF28.1995 |  | MFCSTCVSLVGVISALMLLSYLGFVIIYVPVYRYLAITVLLLLLSYRLSVWRFLPTERAHVRRLAEKIVERRRAYALPTSEELRSDVGANALYVPAAPYL
Tb5.NT.82 | orf_1_243_560 | 106 aa | Tcruzi, Lmajor hypothetical protein | MGFFDSDLSYPTVRTERMKHKRRRLVQGPNSYFMDVKCPGCKNITVVYSHATSEVKCNGCATMLCRPTGGKAILVTGCGFRKKPDH | Tc00.[PHONE].25 | LmjF16.0650 |  | MSNTNSSGCMDVSIPVTLRGAALFCTHEHSELQSILCLICRAHLADHCVHCSTSSGFSLPTSDCLVVKGECGHKFHAHCIGDWGEQHQVCPACRKQWVAAERIARS
Tb7.NT.16 | orf_1_728_1855 | 376 aa | Tcruzi, Lmajor hypothetical protein | KLSRSRTFERGVPQGTVLGPIMFIIVMNSLSQRLAEVPLLQHGFFADDLTLLARHTERDVINQTLQCGLNVVLQWSKEYFMSI-NVAKTKFTLFGCIGRHPLTLQLDGERIGADRTPKLLGVTFQCLQGMATHATETRRKMDFRLLQITVISASIWGPIRQVLRAFYLALVQARTMCGIEVWYWDASERSRDLLAAAQHKASRIIAGIPHGTRKE | Tc00.[PHONE].66 | LmjF22.0160 | Tb927.7.2140
Tb7.NT.18 | [NAME] | 1,370 aa | Lmajor hypothetical protein | RKTILLAAPSEAEPRFHSLPRGCIVPHSSMEDTLSSFLDQLAELQVETMERNRVLKVLNTEVEAAKDLKERKVSDSQRIASDEANSPADIVEAPISVIPSRNLTRKSLMFALRAVGVEKAKERYKILEDVDTVVPREKARTILEYYGSRCLTEKVMSMFVAYDEFYSGEVPLSIAFEILEYCGLVTQTVGKFKIFLRPQVSQGQILLCVADQPQEATENIVDDAEGEKRWIQPQTYETT----VRCCGEGWKYGDH-KVYWNGESGDVFVGSFIVAKGITERKVAVQLLRYIEAFLYVCHAIMVVTVGTGMKLRVLYTPLVEK--------LLNPTVKGVTDATS----FLY-ELAFGSVFNPKKYVKLLANNS---NLRDRYLCEGDDAVLHDT--IRPKHLVPKAPFMINGDKDDELNTLLMSTANSYTTVSANIWTQKGVEGCYLEVGVEKLRISEKVPENARCYCLVSVRKSKDSEWMTAEV-LQAVSVGKANXXXXXXXXXXXXXDR----LFFTGAIEDSLYVEACYDIVDA----------------------SGETTTYCVGHVLVPCGKLKSGKLPVRPGSCYTYTPLDE------------ALKQPVEASGGFCAPRRKTAGPPA--KSLTVTIKRLRSKD-VPDLKTLPSRCLSLHRHVSLMSLMCAAV-ISGRDGGSHPLRWLRQQQVQCCFMVTADSELMDILQGLWKRVMKGKLGPKPQNLGQKYKRILILATKVVAAHNNITGGDQGIITDVVAGK | no | [NAME] | Tb927.7.2150
Tb9.NT.72 | [NAME] | 118 aa | Tcruzi, Lmajor hypothetical protein | MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGGVMEPTLEALAKKYNWEKKVCRRCYARLPVRATNCRKKGCGHCSNLRMKKKLR | Tc00.[PHONE].26 | LmjF35.2580 |  | MAMSGDGGGGGEKPPLGLDERTRQNMRIAEKIGSECFEQNTQILRGLAWRGLRAFFVFGGGVAAFGYAMRRQRSRKEAMDASGDPTQRYLEEMRGLGFDVDTVEEELEVSRRSGESA
Tb11.NT.10 | orf_1_73_357 | 95 aa | Tcruzi, Lmajor hypothetical protein | EALKPKSKEEQLPI-KNECTLDRREGKQIVAEKWSEVVVDDNESQERSNESVSESQVYDTEEELLAAGEDLSGDALKDFLRKNFVDFIDEKVARVGDLDLM | Tc00.[PHONE].64 | LmjF25.0660 |  | [NAME]
Tb11.NT.100 | orf_1_141_434 | 98 aa | Tcruzi, Lmajor hypothetical protein | MFCST--CVSLVGVISALMLLSYLGFVIIYVPVYRYLAITVXXXXXSYRLSVWRFLPTERAHVRRLAEKIVERRRAYALPT | Tc00.[PHONE].79 | LmjF28.0600 |  | MSQKGLKGKTRGKSEGALRKYVGEAKKKTVFNKGRKTLQTKARANYISAVESHMASRVPSDQRDRLRVVKATGGLQPKKKHMKKPLTRGRKRKGDKK
Tb11.NT.118 | orf_1_15_227 | 71 aa | 60S ribosomal protein L29 | MLLGGFVPRRFSQFNRDPCWMFFIFSVGFWLGEYPAMMIKYNARDLVYDPHRYVWSHHDDHH | Tc00.[PHONE].30 | LmjF36.3390 | Tb11.01.1790 | MAKSKNHTNHNQSRKNHRNGIKPPLPLYMYNSKRGGWLPALVNTRRVRKNNQKAALKARRERLAAHQAAQK
Tb11.NT.126 | orf_1_20_358 | 113 aa | Tcruzi, Lmajor hypothetical protein | MLRRAGSRVACACSVP---QARSLHFPITPPPIEIEYLDNDPLEFAVRTEARKWRFDDMGYMRELAFVRINNNPTVGDFRNMSPDERRNLFWGSDRQDFFRHLTCTLTGSPEHLYHRGW | Tc00.[PHONE].80 | LmjF28.2970 |  | [NAME]
Tb11.NT.128 | orf_1_172_462 | 97 aa | Tcruzi, Lmajor hypothetical protein | CTVVAGSNVLPLFSLLLSFITPLPFLFFGR----SESTFDDDGEINGFV---VFLSGALAVSAPSLSIVLYHTXXXXXXXXXXXXXXQVTLLGAAAFLQSGERQDEEGN | Tc00.[PHONE].104 | LmjF28.2720 |  | MAEKIASDKITLHLAAKTGITEALGNMECDPAETLGKVLSRIKKKLKRPALYLFLMRGSEGFIPTPDQTIGSLLHAYAESSDQRHLSFAVSTAIFHG
Tb11.NT.133 | orf_1_140_409 | 90 aa | [NAME] chain | RVWNLMDIRR--RPPVLNRLRGVLQFAQQ--PGALSRHRQGGDYNCSMRVSIYSRPG-KVSRLNNADATWHNRSRKEKPPDFDPSAFRRRYRDS | Tc00.[PHONE].104 | LmjF28.2130 | Tb11.0845 | MTQLPSATIKLIEMPKHMENYAVNCAQDGFTKYYTEKDVASHIKKEFDKKYGPTWNCFVGRNFASYVTHETKHYIYFYMGQMGVLLFKSS
Tb11.NT.135 | [NAME] | 57 aa | ribosomal protein S29 | MSGIRVAIFSKYFAAGVTVGALLCSRHCSWMLATHHADIQQHYGNIMRYAEQVTSLEE | Tc00.[PHONE].15 | LmjF28.2205 |  | MGHLHQWRSRQKIGMGKGSRHCVICSNQKALIRKYELNVCRQCFRENAENIGFVKLR
Tb11.NT.138 | orf_1_95_265 | 57 aa | ribosomal protein S29 | CVICLEQWSDPVELLPCTHIFCRGCVSTATVCPICRAEVTGLRTANRYLVDASMSL | Tc00.[PHONE].15 | LmjF28.2205 |  | MGHLHQWRSRQKIGMGKGSRHCVICSNQKALIRKYELNVCRQCFRENAENIGFVKLR
Tb11.NT.151 | orf_1_28_828 | 337 aa | ribonucleotide reductase | MGKRDLEKERQIELAAIWGAEVDGENRFPRRRGLSGKRSTGASCLXXXXXXXXXXXXXXDLGSETRSEECNNXXXXXXXXXXXXXXXTNSTYRLHCFVGPGTERNDVRTVFEAYEPKVELRTSQQGSLLNKTHFAVLTFRNKAMALHAVKVLDGTNQYDRLGVRKLKLGLMLSRKEHSKIRQKLRKRMREDHERRLAEEVSEEAEFVKKFIESYVPSAG | Tc00.[PHONE].80 | LmjF27.2050 | Tb11.v4.0005
Tb11.NT.154 | orf_1_41_424 | 128 aa | ubiquitin-fusion protein | FQLDWECSYQEYMKMIYHHATNYVPPRVSQERHLITCRELLNEAQRECKGGKYDLSFFHYLRCMEILTKVGIPSTYKDSMVLKK----QCMDAIEALMNSALKNHYEKMVKELKSKK | Tc00.[PHONE].4 | LmjF31.1900 |  | MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGGVMEPTLEALAKKYNWEKKVCRRCYARLPVRATNCRKKGCGHCSNLRMKKKLR
Tb11.NT.155 | [NAME] | 128 aa | ubiquitin-fusion protein | MRGNRLARRGDPMKTXXXXXXXXXXXXXXXXWAQRARKRLCRVIAKRCAREEDSHMTAIMQSYAI | Tc00.[PHONE].4 | LmjF31.1900 |  | MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGGVMEPTLEALAKKYNWEKKVCRRCYARLPVRATNCRKKGCGHCSNLRMKKKLR
Tb11.NT.159 | orf_1_36_383 | 116 aa | Lmajor hypothetical protein | AMMIRLIEEQIWCGYEDVETAVNASCEGENVTYLRENVFPTLVPALYELVKRQQQCALEPDEVSHRYGPTGNTHPISWLSQYLLRNNTRH-SKKICGHPYVMVNNELL | no | LmjF23.0370 | Tb11.01.4702 | MLRRAGSRVACACSVPQARSLHFPITPPPIEIEYLDNDPLEFAVRTEARKWRFDDMGYMRELAFVRINNNPTVGDFRNMSPDERRNLFWGSDRQDFFRHLTCTLTGSPEHLYHRGW
Tb11.NT.186 | orf_1_159_605 | 154 aa | Tcruzi, Lmajor hypothetical protein | MLRRSYSRLGHQLIKEPLGHEQTTFARGQRTMANTLKGFYALCAFSAVGCVFASYSLVTGMNRVYPCVNSKGTLSKDNSPFLWWKY | Tc00.[PHONE].40 | LmjF32.1800 |  | MRGNRLARRGDPMKTRSERRRERKKKISSSSWAQRARKRLCRVIAKRCAREEDSHMTAIMKSHRRVDARRYPGREAKALRERQSKARTQSLY
Tb11.NT.191 | orf_1_22_411 | 130 aa | Tcruzi hypothetical protein | RTRQNMRIAEKIGSECFEQNTQILRGLAWRGLRXXXXXXXXXXXXXXXMRRQRSRKEA-----MDASGD--------PTQRYLEEMRGLGFDVDTVEEELEVSRRSGESA | Tc00.[PHONE].20 | no |  | [NAME]
Tb11.NT.20 | orf_1_70_486 | 139 aa | Tcruzi, Lmajor hypothetical protein | MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGGVMEPTLEALAKKYNWEKKVCRRCYARLPVRATNCRKKGCGHCSNLRMKKKLR | Tc00.[PHONE].40 | LmjF27.1270 | Tb11.46.0014 | MSFFQLDWECSYQEYMKMIYHHATNYVPPRVSQERHLITCRELLNEAQRECKGGKYDLSFFHYLRCMEILTKVGIPSTYKDSMVLKKQCMDAIEALMNSALKNHYEKMVKELKSKKEGVDAVDYKLYGDRLAHVSGGNS
Tb11.NT.47 | orf_1_243_455 | 71 aa | Tcruzi, Lmajor hypothetical protein | SETRVEVLQRTLQLLRQEILVPYFEPVERLRVTNNRAAYVHDIVDALHRS | Tc00.[PHONE].10 | LmjF13.1120 |  | MIIPVRCFTCGNVIADKYLLYLDLVAEGSSEEAALDAFNLWRFCCRRMFLTHVDFSDLLLKYNPADSSVAV
Tb11.NT.57 | orf_1_65_841 | 259 aa | Tcruzi, Lmajor hypothetical protein | LASHQPILPPSVSTRVAVMRFLGDGRSRMPXXXXXXXXXXKTGAKGLAGNASVLGKAMFYLKRGNPVGAIAEIDKILPEISDDALKRFAVGIRLRARNDLLDMKESALKVHGGITELEVSDIRTALRDDYKVLEAASPGCWLFELAVAEYRLYEGCAKDAYDSFRLVEKSIQEYILSLKKPSP---SQALHGTDNHASLVL-AFQLSRARTPNVPKVSSKIVDEAIKGIVEGYSTKEATLAFKKDLGAALTDEEVVELVYIFEAAHIRHHFHDYFPLHED-YRGWGSERANAAKGTLASHSTS-SVFLDDKLLASIRTSVPHQPFYAGEDELRRFMSSDHKD--LIAELRHTFGSGPKTSLAKSEDAYFDALKAVENSPMSADAAGSAAYPVSQNDICLCLAQQLLYRTRVNIAVALTQLDRLEEAVPILDGVINADEYIYMWRALLARGEANKRFGHIGKSDEDFRQLLQLKR | Tc00.[PHONE].110 | LmjF13.0140 | Tb11.02.2250 | MRLHTVRAPIITRAAMRGYSEARSNYDGTSLPAWPAPGKKPTYPAALSELRLPQPRMRKTRTEWMYYHGHGGCPGKYGPSREIADFEYADGTPASISGRRFAFKHHQDHLLVQLIRAAATVERYDASGLLPRIPGTAEQRNWDPAIPLFLDDVDEQGRPAPLRTAGDAPGTMVSHVCSRVVDERMGTPTHTPNELANRHEGETLEANTMFATNDPSAFVSDTVKLRDDKRPYWSRRRWALTDKFLVPKSPKPKNTIKDE
[NAME] | orf_1_90_746 | 219 aa | Tcruzi, Lmajor hypothetical protein | AYVDALDHLSTKLRIPGSAVEPTNAMYDDGLVPGVCRLYMEGRCRQGSRCFQVHANPEVVEKLRQEALEQPTCCFFHGAPCTSSGLPLNLQV-----------------------------------------------NVGGT----SITLPRMNSTNCLWSAYAERGDSTLDLPREKVCREHRRGLCRFGEECGFLHICREIAF-VGED------------ASSTGSGLHHSRTSASASPLGS | Tc00.[PHONE].100 | [NAME] |  | [NAME]
Tb11.NT.95 | orf_1_57_338 | 94 aa | Tcruzi, Lmajor hypothetical protein | MTQLPSATIKLIEMPKHMENYAVNCAQDGFTKYYTEKDVASHIKKEFDKKYGPTWNCFVGRNFASYVTHETKHYIYFYMGQMGV | Tc00.[PHONE].35 | LmjF28.1170 |  | MSVVCAFKGCSNLTYTALPACEHCSQRMCTSHLLPEVHGCGDRAKNVAQRKATADAAEQRQQRKHIGLDDAKARLTRRREELAAQRQKKPIKKK