Spreadsheet: 0539c4f2-0ea9-47d4-897b-31c251db2955
Sheet: Sheet1

Supplementary table 5: Signifcantly upregulated pathways analyzed by Gene Set Enrichmen Analysis (GSEA).
 |  |  | ST3_STV |  | ST6_STV |  | ST6_ST3 |  | SR3_SRV |  | SR6_SRV |  | SR6_SR3 |  | STV_SRV |  | [NAME] |  | ST6_SR6
KEGG ID | Specific Pathway | General [NAME] | down | up | down | up | down | up | down | up | down | up | down | up | SR up | ST up | SR up | ST up | SR up | ST up
DME00252 | Alanine and aspartate metabolism | amino acid metabolism | X |  |  |  |  |  |  |  | X |  |  |  |  |  |  |  |  | X
DME00330 | Arginine and proline metabolism | amino acid metabolism | X |  | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00251 | Glutamate metabolism | amino acid metabolism | X
DME00260 | Glycine, serine and threonine metabolism | amino acid metabolism | X |  |  |  |  | X |  |  | X |  | X |  |  |  |  | X |  | X
DME00340 | Histidine metabolism | amino acid metabolism | X |  |  |  |  | X |  |  | X
DME00310 | Lysine degradation | amino acid metabolism |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00271 | Methionine metabolism | amino acid metabolism |  |  |  |  |  |  |  |  |  |  | X
DME00360 | Phenylalanine metabolism | amino acid metabolism |  |  | X |  |  | X |  | X | X |  | X |  |  |  | X |  |  | X
DME00380 | Tryptophan metabolism | amino acid metabolism | X |  |  |  |  | X |  |  | X |  | X |  |  |  |  |  |  | X
DME00350 | Tyrosine metabolism | amino acid metabolism | X |  | X |  |  | X |  | X | X |  | X |  |  |  | X |  |  | X
DME00220 | Urea cycle and metabolism of amino groups | amino acid metabolism |  |  |  |  |  | X |  |  | X |  | X |  |  |  |  |  |  | X
DME00290 | [NAME], leucine and isoleucine biosynthesis | amino acid metabolism |  |  |  |  |  |  |  |  |  | X
DME00280 | [NAME], leucine and isoleucine degradation | amino acid metabolism |  |  |  |  |  | X |  |  |  |  |  |  |  |  |  |  |  | X
DME00960 | Alkaloid biosynthesis II | biosynthesis of secondary metabolites | X |  | X |  |  | X |  | X | X |  | X |  |  |  |  |  |  | X
DME00903 | [NAME] and pinene degradation | biosynthesis of secondary metabolites | X |  | X |  |  | X |  | X |  |  |  |  |  |  |  |  |  | X
DME00530 | Aminosugars metabolism | carbohydrate metabolism |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00650 | Butanoate metabolism | carbohydrate metabolism | X |  |  |  |  |  |  |  |  |  | X |  |  |  |  |  |  | X
DME00020 | Citrate cycle (TCA cycle) | carbohydrate metabolism | X |  | X |  |  |  | X |  | X |  |  |  |  |  |  |  |  | X
DME00052 | Galactose metabolism | carbohydrate metabolism | X |  |  |  |  |  |  |  | X |  | X |  |  |  |  |  |  | X
DME00010 | Glycolysis / Gluconeogenesis | carbohydrate metabolism | X |  |  |  |  | X |  |  | X |  | X |  |  |  |  |  |  | X
DME00630 | Glyoxylate and dicarboxylate metabolism | carbohydrate metabolism | X |  | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00562 | Inositol phosphate metabolism | carbohydrate metabolism |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00040 | Pentose and glucuronate interconversions | carbohydrate metabolism | X
[NAME] | Pentose phosphate pathway | carbohydrate metabolism | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00640 | Propanoate metabolism | carbohydrate metabolism | X |  | X |  |  | X |  |  |  |  |  |  |  |  |  |  |  | X
DME00620 | Pyruvate metabolism | carbohydrate metabolism | X |  |  |  |  |  |  |  | X |  | X |  |  |  |  |  |  | X
DME00500 | Starch and sucrose metabolism | carbohydrate metabolism | X |  | X |  |  | X |  |  |  |  |  |  |  |  |  |  |  | X
DME04110 | Cell cycle | cell growth and death |  |  |  |  |  |  |  |  |  | X
[NAME] | Nitrogen metabolism | energy metabolism | X |  | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00190 | Oxidative phosphorylation | energy metabolism | X |  | X |  |  |  | X |  | X |  | X |  | X |  | X |  |  | X
DME00720 | Reductive carboxylate cycle (CO2 fixation) | energy metabolism | X |  | X |  |  |  |  |  | X
DME03050 | Proteasome | folding, sorting and degradation |  |  |  |  |  | X |  |  |  |  |  |  |  |  |  |  |  | X
DME03060 | Protein export | folding, sorting and degradation |  |  |  | X |  |  |  | X |  |  |  |  |  |  |  |  |  | X
DME04130 | SNARE interactions in vesicular transport | folding, sorting and degradation |  |  |  |  |  |  |  | X |  | X
DME04120 | Ubiquitin mediated proteolysis | folding, sorting and degradation |  |  |  | X |  | X
DME01032 | Glycan structures - degradation | glycan biosynthesis and metabolism | X |  | X |  |  | X
DME00531 | Glycosaminoglycan degradation | glycan biosynthesis and metabolism | X |  | X |  |  | X |  |  |  |  |  |  | X |  | X
[NAME] | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis | glycan biosynthesis and metabolism |  |  |  |  |  | X
DME00510 | N-Glycan biosynthesis | glycan biosynthesis and metabolism |  |  |  | X |  | X |  | X |  |  |  |  |  |  |  |  |  | X
DME00511 | N-Glycan degradation | glycan biosynthesis and metabolism | X |  | X
DME00592 | alpha-Linolenic acid metabolism | lipid metabolism |  |  |  |  |  |  |  | X |  | X
DME00150 | Androgen and estrogen metabolism | lipid metabolism | X |  | X |  |  | X
DME00120 | Bile acid biosynthesis | lipid metabolism | X |  |  |  |  | X
DME01040 | Biosynthesis of unsaturated fatty acids | lipid metabolism
DME00565 | Ether lipid metabolism | lipid metabolism |  |  |  |  |  | X |  | X |  |  |  |  |  |  |  |  |  | X
DME00061 | Fatty acid biosynthesis | lipid metabolism |  |  |  |  |  |  |  |  |  |  | X
DME00071 | Fatty acid metabolism | lipid metabolism | X |  | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00561 | Glycerolipid metabolism | lipid metabolism | X |  |  |  |  |  |  |  |  |  | X |  |  |  |  |  |  | X
DME00564 | Glycerophospholipid metabolism | lipid metabolism |  |  |  |  |  | X |  | X |  |  |  |  |  |  |  |  |  | X
DME00591 | Linoleic acid metabolism | lipid metabolism |  |  |  |  |  |  |  | X | X | X | X
[NAME] | Sphingolipid metabolism | lipid metabolism |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X |  | X
DME00072 | Synthesis and degradation of ketone bodies | lipid metabolism |  |  |  |  |  |  |  |  |  |  | X |  |  |  |  |  |  | X
DME00790 | Folate biosynthesis | metabolism of cofactors and vitamins | X |  |  |  |  |  |  |  | X |  | X |  |  |  |  |  |  | X
DME00760 | Nicotinate and nicotinamide metabolism | metabolism of cofactors and vitamins | X |  |  |  |  |  | X |  | X
DME00670 | One carbon pool by folate | metabolism of cofactors and vitamins |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X |  | X
DME00860 | Porphyrin and chlorophyll metabolism | metabolism of cofactors and vitamins | X |  |  |  |  | X
DME00440 | Aminophosphonate metabolism | metabolism of other amino acids |  |  |  |  |  | X
DME00410 | beta-Alanine metabolism | metabolism of other amino acids | X
DME00460 | Cyanoamino acid metabolism | metabolism of other amino acids |  |  | X |  |  |  |  |  |  |  |  |  | X |  |  |  |  | X
DME00480 | Glutathione metabolism | metabolism of other amino acids | X |  | X
DME00450 | Selenoamino acid metabolism | metabolism of other amino acids |  |  | X |  |  |  |  |  |  |  |  |  | X |  | X
DME00430 | Taurine and hypotaurine metabolism | metabolism of other amino acids |  |  | X |  |  |  |  |  |  |  |  |  | X
DME00230 | Purine metabolism | nucleotide metabolism |  |  |  | X |  | X |  | X |  | X
DME00240 | Pyrimidine metabolism | nucleotide metabolism |  |  |  | X |  |  |  |  |  |  | X |  |  |  |  |  |  | X
DME03410 | Base excision repair | replication and repair |  |  |  | X |  |  |  | X |  | X
DME03030 | DNA replication | replication and repair |  | X |  | X |  | X |  | X |  | X |  | X
DME03430 | Mismatch repair | replication and repair |  |  |  | X |  | X |  | X |  | X
DME03420 | Nucleotide excision repair | replication and repair |  |  |  | X |  | X |  | X |  | X
DME04070 | Phosphatidylinositol signaling system | signal transduction |  |  |  |  |  |  |  |  |  |  |  |  | X
DME03020 | RNA polymerase | transcription |  |  |  | X |  | X |  | X |  |  | X |  |  |  |  |  |  | X
DME00970 | Aminoacyl-tRNA biosynthesis | translation |  |  |  | X |  | X |  |  |  | X
DME00624 | 1- and 2-Methylnaphthalene degradation | xenobiotics biodegradation and metabolism | X |  | X |  |  | X |  | X | X |  | X |  |  |  |  |  |  | X
DME00632 | Benzoate degradation via CoA ligation | xenobiotics biodegradation and metabolism | X |  | X |  |  | X |  | X | X |  | X |  |  |  |  |  |  | X
DME00930 | Caprolactam degradation | xenobiotics biodegradation and metabolism | X |  | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
[NAME] | Drug metabolism - cytochrome P450 | xenobiotics biodegradation and metabolism | X |  | X |  |  | X
DME00983 | Drug metabolism - other enzymes | xenobiotics biodegradation and metabolism | X
DME00642 | Ethylbenzene degradation | xenobiotics biodegradation and metabolism | X |  | X |  |  | X |  | X | X |  | X |  |  |  |  |  |  | X
DME00361 | [NAME] degradation | xenobiotics biodegradation and metabolism | X |  |  |  |  |  |  |  | X |  | X |  |  |  |  |  |  | X
[NAME] | Metabolism of xenobiotics by cytochrome P450 | xenobiotics biodegradation and metabolism | X |  | X
DME00626 | Naphthalene and anthracene degradation | xenobiotics biodegradation and metabolism | X |  |  |  |  |  |  |  | X |  |  |  |  |  |  |  |  | X
DME00643 | Styrene degradation | xenobiotics biodegradation and metabolism |  |  | X |  |  |  |  |  |  |  |  |  |  |  |  |  |  | X
DME00710 | Carbon fixation |  | X |  | X |  |  | X | X |  | X |  |  |  |  |  |  |  |  | X
DME00940 | Phenylpropanoid biosynthesis |  |  |  |  |  |  |  |  | X
Pathways identified using GSEA as significantly differentially expressed ([NAME] ≤ 0.25). The direction of regulation is relative to the first category in each comparison, for example in the comparison of [NAME] the pathway of amino acid metabolism is down-regulated in ST3. down = significantly down-regulated; up = significantly up-regulated; ST = spermathecae, SR = Seminal receptacle, V = virgin, 3 = 3 hours post-mating, 6 = 6 hours post-mating.