Spreadsheet: 151e628a-e66a-43b6-a8b5-4386a348d9c4
Sheet: Primers1

OGS number | GT (Gland transcriptome) number | Usage of primers | Primer-forward | Primer-reverse | Expected length(bp) | Ta-Taq polymerase | Ta-Phusion polymerase
TC000889 | GT001 | dsRNA | ATAGGTATGTGACGGACTTGTGGACT | TGATGTCAGCGTTCTCTGTCTTTGT | 1070 | 63 | 70
TC000917 | [NAME] | dsRNA | ATGTCCATCACTTACCCATAAACCAG | CGAGGATAAGTTTTGCGATGCC | 624 | 63 | 70
TC001815 | GT003 | dsRNA | GTAGAAACTACAAGGTGTCAACTGTCA | CTTCTCGCAATCGGTTCGTT | 235 | 60 | 67
TC001937 | GT004 | dsRNA | CGGTTAGCACTGTCAAACGAATCC | GTCGGGTTTTGCTACAACTTTAGGC | 740 | 65 | 71
TC002417 | GT005 | dsRNA | CTCTGCCAAAATCCTGTATGCTAAT | ATTCCGAGCGTAGTCTTGGGTT | 897 | 62 | 68
TC002483 | GT006 | dsRNA | GCCACTCTGAACAAAAAAGACGACTC | GGTCGAGTCTGGACTTTTGCCTG | 698 | 65 | 72
TC002669 | GT007 | dsRNA | ACGACAACAAAAACGCCTACAATG | ATTTACTGTCGTCGTCCTTTATGGC | 648 | 63 | 70
TC002938 | GT008 | dsRNA | CTTGCACCAAAAAGTGTAACGAGCC | AGAACACTTCAAGCAACGCCTCCT | 585 | 66 | 73
TC003110 | GT009 | dsRNA | GCCAATAATAAAGTATGATGATGCT | TTTCTTCATCATTAACTTCTACGTTTT | 434 | 58 | 64
TC003112 | [NAME] | dsRNA | CTATGACAAAAATCTTTCAAACCCTG | ATCTTTAGGTGTTTCCACAATCTCATC | 409 | 62 | 68
TC003284 | GT011 | dsRNA | GCATAATCACCCAAACCTCTGTCCT | CACACAAGACCTGCCACAACAACTAT | 610 | 65 | 72
[NAME] | GT012 | dsRNA | TTTGGAAAATACGGTCCGAGTGGT | GTCTTGAAACATCGCCAGCATTACA | 778 | 66 | 73
TC003771 | GT013 | dsRNA | GATCACCGGCCATTTTATTGTCGT | GGCATTTTTTCAGAATAACCTGCTCCT | 232 | 67 | 72
TC003835 | [NAME] | dsRNA | CGCCTCGACAACCTGTACCTGCC | CCACCTCCACTTCCACCCCCACTA | 410 | 70 | 77
TC004085 | GT015 | dsRNA | ACCGAAATAATCGAAAAACACATAGGT | AAGGGGCAAGATATTTCACAGGAG | 520 | 63 | 69
TC004334 | [NAME] | dsRNA | CTCCTCCCGATAACGAACCCCTC | CCAGCTCGGACATAGTTTTAACGGC | 165 | 67 | 74
TC004655 | GT017 | dsRNA | GGCCTGTGCCTGTTGTTAATTTCCC | GAGACAGGGCACTTTCAGAATGGGC | 345 | 69 | 75
TC005106 | GT018 | dsRNA | GCGGTCATTTCAGACTTGGGCAC | TTTAAGGGGGCCAGAATCTAGAGCAG | 879 | 68 | 74
TC005384 | GT019 | dsRNA | GGACTGAAGCAGAAGAATATGGGCG | GGGCTTGGGAAGTTATGTCATCGTC | 877 | 67 | 74
TC005389 | GT020 | dsRNA | AACTGTGGGAACCCCCGAAAC | ATAAGGAGTGAAGAAAAATAGGGCTGTT | 517 | 64 | 69
TC005529 | GT021 | dsRNA | TCTTTTACTGTTTGGTGACCCTTTCG | TGTTCATCCTCTTTTTCCTCGTGCT | 291 | 65 | 72
TC005635 | [NAME] | dsRNA | TAATTGTACGGTCGTCCCAGCATT | GCATCAGAGAGTAGATTAGGTGTCGTGT | 1167 | 64 | 71
TC006131 | GT023 | dsRNA | GGGACGTTTTTTGTACGGTGAGAATT | AACAGTGTTTAAGGGCGACCATGTC | 284 | 66 | 72
TC006800 | GT024 | dsRNA | ATGACAAAGTTTTCAATCTCGCAAG | CATTTTTCCCCAAACTAACTTCCTG | 598 | 63 | 69
TC007254 | GT025 | dsRNA | GAAACCACGACGGAACAAGAAACTAC | AGGCTGGCAAAATTCCTGTTAGAAG | 553 | 65 | 71
TC007317 | GT026 | dsRNA | TCGGTTGCTTTCGGTATTGAGTGTA | AGTCATGTCCATCATGGCTTCATAAGT | 534 | 65 | 71
TC008412 | GT027 | dsRNA | CCATAGTTGCGATGTTTGAAGAGGAT | GCGAATAATCGGCTAAGTTTCCAAA | 798 | 65 | 71
TC008413 | GT028 | dsRNA | CTTTTATGGTCCTTTGCCTGGTTCT | TCTTCCTCTCTTGCATCTTATGTTCGT | 787 | 64 | 71
TC008414 | GT029 | dsRNA | GATTCGCCACGACAACATCCAAG | TGACCATTTCGTATCAACAACCCAA | 620 | 66 | 73
TC008677 | GT030 | dsRNA | ATTTTTGTTAGCTCTTGCTTGCCTC | GTTACATTTTGCACATCCGTTTTGT | 214 | 63 | 69
TC008780 | GT031 | dsRNA | GATGAAAACGAATGGGGACTACAGC | GACACTAGCCTCCGTCCCTAAAATG | 466 | 65 | 72
TC008804 | GT032 | dsRNA | [NAME] | ACACTATTTGTGACAGCCTCCTCCATC | 958 | 67 | 73
TC010236 | [NAME] | dsRNA | GTGGACCCCGACTACATCAAGCAAG | TCACTCTCTTGACTGTCTACAACCGCC | 749 | 68 | 74
TC010286 | GT034 | dsRNA | ACCTCCTAGCCATTCAGGTGGAAGAC | TCTCCTTGGTTGTGACTCCTGGGAG | 95 | 68 | 74
TC010551 | GT035 | dsRNA | AACTTTTGTGCTGTTTATTGTCATTG | TCTGGTAGTAGTGGAAGTAGTTCTTGG | 235 | 60 | 67
TC010909 | GT036 | dsRNA | CACCTTGTGCCTGTTCCTTGTGTAT | CAGCCTCAGGGAGACCATGAATT | 410 | 65 | 72
TC011094 | GT037 | dsRNA | GTGAAGTGATTTGGGTTTTGTGCTG | [NAME] | 459 | 65 | 71
TC011149 | [NAME] | dsRNA | ATGAATACTCTAACGGTCGCTTTTT | GTACCCGAAATGAGTAGTACCAACG | 372 | 61 | 67
TC011211 | GT039 | dsRNA | AACCAGGTATGGGTGGACAAAATG | TGTAACCCCTTCAAATCTATCCTCATC | 528 | 64 | 70
TC011236 | GT040 | dsRNA | CGACTTTTCGTGACTGTATTTGGGC | CACCACTGACTGCGACAGGAAGAAT | 441 | 67 | 73
TC011337 | GT041 | dsRNA | TGTGATGACGAAGATGACTATGGAA | ATGACAGGTGCTTGTTCACTAGCTC | 590 | 62 | 69
TC012298 | GT042 | dsRNA | TGTTGCCATCCACGAGTGTAGCG | GCATTTTTCTCCATTGTCACCCCAT | 1038 | 68 | 74
TC012613 | [NAME] | dsRNA | AGAGACAGCAAGGTCCACGACAATG | GCCGTTGCTTTTGTATTTCGTTGAG | 658 | 67 | 73
TC012640 | GT044 | dsRNA | GGCAGTCTCCGAAGTCACCAAATAC | TCCTCCAATCACACCCACATTTTTC | 586 | 66 | 73
TC012841 | GT045 | dsRNA | GAGTTTAGCAAAGAAGCGAGTCAGAT | AACAAAGTCAATGTGAGAATAACCCTG | 591 | 63 | 69
TC012946 | [NAME] | dsRNA | GCAGCCAATAACTCTCCCATCAACA | GCGACACCAACCTTGCTCTACCATA | 456 | 67 | 74
TC013059 | [NAME] | dsRNA | ATGCGAGCCGCTTTGATTGTCTTT | GCCACGGTCACGAAGACTTGGTAAT | 416 | 68 | 75
TC013065 | GT048 | dsRNA | GTGCTCGTAAAATCATCAACTGGCG | ATTTTTCGGGGGGCAGTAACATTG | 1040 | 67 | 74
TC013108 | GT049 | dsRNA | GGTTCAGGAAGTGTGACCTTATGGC | GTCTCCAGTAGAATAATGTCCGCACC | 519 | 65 | 71
TC013136 | GT050 | dsRNA | TAGTGTTAGTCGTGTGTTTCGCAGC | CCGTTCTCATCAGCAGTGTAGGC | 268 | 64 | 71
TC013434 | GT051 | dsRNA | ATCTCTTCGTGGCAACACTTTTCCT | CATACTCCTCGCTCGGTTTCTCC | 612 | 65 | 72
TC013474 | GT052 | dsRNA | GCCGTTCTGTCGCAATATAGCAGG | CGGCTTCTCCCCTGTGTGAATGTA | 744 | 67 | 74
TC013630 | GT053 | dsRNA | CGAAACTGAACAGTTCCTTCATGGG | GTTCTATCACATTCGTGCTCAGTCTCAAA | 508 | 66 | 73
TC013755 | [NAME] | dsRNA | AGGCGACTTCTTCAGAGATTGTGGT | AGAATGGTTGCTTAAAGGCATCAGAC | 742 | 65 | 71
TC013827 | GT055 | dsRNA | AGTGCTTGTGTTTCTGACGCCTTGT | GTACTGCATGTCCTTCTTTGCTCACG | 523 | 67 | 73
TC014185 | [NAME] | dsRNA | CAAGCTGCTGAAAAGCGACAAGGAG | GCGGAATAGCGTAGCTGCCGTAAC | 350 | 69 | 75
TC014359 | GT057 | dsRNA | AATATCCTGACAGCAAGTTAGGGAAC | TTCAAAACGTCGTCGTAAGTGTATTT | 442 | 62 | 68
TC014388 | GT058 | dsRNA | GACAAAAACGCAAAAGCACGACA | AAATCCAGAACAGGAGTTGCCAGTC | 1076 | 65 | 72
TC014479 | GT059 | dsRNA | [NAME] | TTTGTCGTCACTGTCCAGACTGTAGGT | 573 | 65 | 72
TC014748 | GT060 | dsRNA | GAGACGAATAGACGAAAAGAGAGAA | TTTCATCTTTGACTATTGTAGCGTAA | 653 | 59 | 65
TC015044 | GT061 | dsRNA | ATTCGGTGCCATTTTGGAAGACTC | ATGTCGTCACAGGTGGTAACACTCTTG | 294 | 66 | 73
TC015151 | GT062 | dsRNA | TTTTACGGATTTTAACAGCACGGC | TATTAGTAGTCTCGCACGGGTCATTTT | 1007 | 65 | 70
TC015346 | [NAME] | dsRNA | CTTCTCACCGAATACGAGCAAACG | TGAGGCAATAATGCGTCAAACAAGT | 1096 | 65 | 72
TC015382 | [NAME] | dsRNA | CCAGAGGGTTCTTTTACAGCCACAT | TACCATGAGACTTTGAACTGTTCCGAC | 220 | 65 | 72
TC015448 | GT065 | dsRNA | TTAACAAACAACCCCATCTTCCGAA | GAAGGGCTTCATCAAGGCTAGGATT | 530 | 66 | 72
TC015546 | GT066 | dsRNA | GTTTCCCCCATCTGTCTAATCGTCG | GGCACTGTCGTGATTCCCATTCTT | 479 | 67 | 74
TC015603 | GT067 | dsRNA | GGAGACACCGAGGCTTGGAGGA | CTGGCTGTGTAGGCATTGATTCCG | 179 | 68 | 75
TC015620 | GT068 | dsRNA | CTTTTAGTCACGCCAGCCCTCTC | GCACCCTCTTCCCACTGCTATACTC | 522 | 65 | 72
TC015722 | GT069 | dsRNA | GCTCAGAACCAGTCCCAGACGAC | ACGCCGATGTTGACGATTTTTTTAC | 1216 | 65 | 72
TC015886 | GT070 | dsRNA | GACGATTTCTTGCTAATGGATAACTC | TATAGGCGACTGCTGTAGTATTGTCA | 685 | 61 | 67
TC015918 | [NAME] | dsRNA | TTCCACGACTGTATTAAAACCTTCCTGA | CAAACGGAACATTCTCATCCACCA | 634 | 66 | 72
TC015932 | GT072 | dsRNA | AAAATCTCGAAGCGTTTCTACAACCATG | GAAACGACCAGACCGTGTCCTCAAC | 400 | 68 | 73
TC015950 | [NAME] | dsRNA | TGCGTTTTTGATGTTATTAGGTGCTG | TGTTGTGGCTCAAGAAGTGGATGAC | 260 | 65 | 71
TC016081 | GT074 | dsRNA | TTTACGAAGCCTGGACCTCTGCCT | CAGAGCAATGTGTAAGTAAGCGGCG | 920 | 68 | 74
TC016280 | GT075 | dsRNA | ATGTCATCCCCTGTCCCAGTCGT | GGACCTAAGGCTGCGATCAAATAATAA | 497 | 66 | 71
TC008161 | [NAME] | dsRNA | The clone was obtained from Dr. [NAME] and [NAME]. |  | 393
TC008674 | GT077 | dsRNA | The clone was obtained from Dr. [NAME] and [NAME]. |  | 375