Spreadsheet: 019d53d5-7775-4d4b-8997-6fb1f65f63a6
Sheet: Sheet1

Test Referenced | Entry Type | Mayo ID | Description | Reporting Description | Method | Collection Container | Temperature | Stability | [NAME] | Unit of Measure | [NAME] | Minimum Volume | Reject Due To | Reference Values | Days/Times Performed | Analytic Time | Maximum Lab Time | CPT Units | CPT Code | LOINC® Code | LOINC® Attributes | Always Performed | Orderable Separately | Performing Location
PPFWE | Test | PPFWE | Protoporphyrins, Fractionation, Washed Erythrocytes | Protoporphyrins, Fractionation, RBC | High-Performance Liquid Chromatography (HPLC) with [NAME] separate quantitation of zinc protoporphyrins and free protoporphyrins. | Green-Top Tube (Sodium Heparin): GrnNaHp |  |  | 5 | mL | All porphyrin tests on erythrocytes can be performed on 1 draw tube.    Collection Container/Tube:  Preferred: Green top (sodium heparin) Acceptable: Metal free, lavender top (EDTA) or green top (lithium heparin) Submission Container/Tube: Plastic vial Specimen Volume: Washed erythrocyte suspension Collection Instructions: 1. Patient should abstain from alcohol for 24 hours. 2. Collect and process whole blood specimen as follows: a. Immediately place specimen on wet ice. b. Transfer entire specimen to a 12-mL graduated centrifuge tube. [NAME] specimen for 10 minutes at 2,000 rpm. d. Record volume of packed cells and the total volume of the specimen. [NAME] supernatant plasma. f. [NAME] packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing. g. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix. Additional Information:  1. Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen. 2. Include a list of medications the patient is currently taking. Forms: 1.	1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions. 2.	2. If not ordering electronically, submit a Biochemical Genetics Request Form (Supply T439) with the specimen. | 1 mL of washed and resuspended e | Hemolysis	NA Thawing	Warm reject; Cold reject Lipemia	NA Icterus	NA Other	Cell suspension not available | FREE PROTOPORPHYRIN <20 mcg/[NAME] packed cells  ZINC-COMPLEXED PROTOPORPHYRIN <60 mcg/[NAME] packed cells See The Heme Biosynthetic Pathway in Special Instructions. | Monday through Friday, 8 a.m. | 2 days (not reported on Saturday or Sunday) | 3 days | 1 | 84202 |  |  |  |  | Rochester
PPFWE | [NAME] | WRBC | Washed RBC |  |  |  | Frozen | 14 days
PPFWE | Order Question | [NAME] | Total cell suspension |  | Plain Text
PPFWE | Order Question | BG572 | Packed cell volume |  | Plain Text
PPFWE | Result | 31932 | Zinc-Complexed Protoporphyrin | Zinc-Complexed Protoporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 2895-1 | Protoporphyrin.zinc:MCnc:Pt:RBC:Qn:
PPFWE | Result | 31933 | Free Protoporphyrin | Free Protoporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | In Process
PPFWE | Result | INTP6 | Interpretation | Interpretation | Alphanumeric
PPFWE | Result | [NAME] | Total cell suspension | Total cell suspension | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | In Process
PPFWE | Result | BG572 | Packed cell volume | Packed cell volume | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | In Process