Spreadsheet: 6bfcdd84-b8fa-4aa1-a162-00a232239052
Sheet: Sheet1

Table S2. Experimental validation of miR-34a target genes. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8
 |  | Pull-down enrichment ratio |  | mRNA down-regulation after miR-34a over-expression
 | Gene name | HCT116 | K562 | HCT116 | K562
Known targets | ACSL1 | 0.235449982 | 2.392713263 | 0.851427489 | 0.999893015
 | ACSL4 | 8.421079992 | 84.89581553 | 0.510611028 | 0.556566703
 | AXL | 16.96500242 | 18.3234053 | 0.34967983 | Absent
 | BCL2 | 1.78 | 1.05 | Absent | Absent
 | BIRC5 | 1.765350893 | 2.14085242 | 0.459508871 | 0.765067566
 | CCND1 | 3.66 | 40.29 | 0.8264462809917356 | Absent
 | CCNE2 | 3.65 | 6.69 | 0.3125 | 0.7299270072992701
 | CD44 | 1.387453329 | 2.370462369 | 1.027772568 | 0.928089582
 | CDK4 | 3.45 | 2.73 | 0.4237288135593221 | 0.6329113924050632
 | [NAME] | 22.8 | 62.52 | 0.3460207612456747 | 0.4975124378109453
 | DLL1 | 8.3 | Absent | Absent | Absent
 | E2F3 | 2.71 | 5.2 | 0.5882352941176471 | 0.7194244604316548
 | EFNB1 | 5.326698546 | 3.881909452 | Absent | Absent
 | FOXP1 | 16.98 | 6.69 | 0.5847953216374269 | 0.6097560975609756
 | HNF4A | 8.35 | Absent | Absent | 0.6451612903225806
 | JAG1 | 0.99 | Absent | 1.149425287356322 | Absent
 | LDHA | 1.431293613 | 1.169495538 | 0.938372885 | 0.920854823
 | LEF1 | Absent | 32.65123086 | Absent | 0.545864966
 | MAGEA2 | Absent | Absent | Absent | Absent
 | MAGEA3 | 1.19863966 | 0.994838754 | 1.02126066 | 0.92757674
 | MAGEA6 | 1.271017251 | 0.951420312 | 1.021581554 | 0.98411646
 | MAGEA12 | Absent | 1.293448061 | 1.039548914 | 0.95945426
 | MEK1 (MAP2K1) | 2.71 | 1.92 | 0.5586592178770949 | 0.7194244604316548
 | MET | 6.77 | 0.53 | 0.3496503496503497 | Absent
 | MTA2 | 8.372052903 | 11.22076677 | 0.656888885 | Absent
 | MYB | 8.94 | 2.47 | 0.3690036900369004 | 0.641025641025641
 | MYC | 1.47 | 4.8 | 1.063829787234043 | 0.9009009009009008
 | MYCN | Absent | Absent | Absent | Absent
 | NOTCH1 | 7.13 | 3.22 | 0.6756756756756757 | 0.7633587786259541
 | NOTCH2 | 7.18 | Absent | 0.47 | Absent
 | RRAS | 63.86021783 | 32.41541838 | 0.246098504 | Absent
 | SIRT1 | 9.84 | 10.22 | 0.5494505494505494 | 0.6535947712418301
 | TPD52 | 13.30839924 | 1.005794493 | 0.190139981 | Absent
 | VCL | 6.196882846 | 1.812687982 | 0.422874356 | 0.88230511
 | WNT1 | Absent | Absent | Absent | Absent
 | YY1 | 4.83448716 | 52.81919248 | 0.753683874 | 0.802352265
 |  |  |  |  |  | Experimental validation of candidate genes
 |  |  |  |  |  | 3'UTR luciferase | 3'UTR MREs | Protein down-regulation in HCT116
Random genes | ACSM3 | 15.96 | 55 | Absent | 2.127659574468085 | NO | NOT TESTED | NOT TESTED
 | AGBL5 | 3.89 | 14.73 | 0.9615384615384615 | 0.8849557522123894 | YES | NOT TESTED | NOT TESTED
 | AXL | 16.97 | 18.32 | 0.3496503496503497 | Absent | YES | NOT TESTED | NOT TESTED
 | CCNG2 | 6.68 | 21.9 | 0.9433962264150942 | 0.6060606060606061 | YES | NOT TESTED | NOT TESTED
 | CDK4 | 3.45 | 2.73 | 0.4237288135593221 | 0.6329113924050632 | YES | NOT TESTED | NOT TESTED
 | FOXP1 | 16.98 | 6.69 | 0.5847953216374269 | 0.6097560975609756 | YES | NOT TESTED | NOT TESTED
 | MAD2L2 | 4.23 | 8.79 | 0.7407407407407407 | 0.8 | YES | NOT TESTED | NOT TESTED
 | PCYOX1L | 2.89 | 9.66 | 0.8771929824561404 | 1.298701298701299 | YES | NOT TESTED | NOT TESTED
 | PDRG1 | 2.69 | 3.27 | 0.8064516129032259 | 0.7518796992481203 | YES | NOT TESTED | NOT TESTED
 | PSMD5 | 2.64 | 2.61 | 0.62 | 0.76 | YES | NOT TESTED | NOT TESTED
 | RBBP4 | 4.65 | 4.18 | 0.4926108374384237 | 0.8333333333333334 | YES | NOT TESTED | NOT TESTED
Growth factor genes | ARAF | 8.29 | 14.64 | 0.4329004329004329 | 0.6896551724137931 | YES | YES | YES
 | AXL | 16.97 | 18.32 | 0.3496503496503497 | Absent | YES | YES | YES
 | MEK1 (MAP2K1) | 2.71 | 1.92 | 0.5586592178770949 | 0.7194244604316548 | YES | YES | YES
 | MET | 6.77 | 0.53 | 0.3496503496503497 | Absent | YES | YES | YES
 | PIK3R2 | 2.33 | 2.3 | 0.6289308176100629 | 0.8928571428571428 | NOT TESTED | YES | YES
Cell cycle genes | CCND3 | 4.45 | 2.9 | 0.7692307692307692 | 1.149425287356322 | YES | YES | Antibody not available
 | MCM2 | 2.29 | 1.44 | 0.4464285714285714 | 0.9259259259259258 | YES | YES | YES
 | MCM4 | 2.27 | 1.84 | 0.6993006993006994 | 0.9615384615384615 | NO | NO | YES
 | MCM5 | 2.83 | 2.44 | 0.4016064257028112 | 0.970873786407767 | YES | YES | YES
 | [NAME] | 3.5 | Absent | Absent | Absent | NO | NO | YES
 | PLK1 | 3.32 | 1.84 | 0.7407407407407407 | 0.8771929824561404 | YES | NO | YES
 | [NAME] | 4.65 | 4.81 | 0.7299270072992701 | 0.684931506849315 | YES | YES | YES