Spreadsheet: 03394494-423f-49e1-a700-9cf7babba14a
Sheet: Sheet1

Table S3. Experimentally derived requirements and estimated effluxes from intermediate and amino acid pools for biomass synthesis of Mycobacterium bovis BCG (BCG) and Mycobacterium tuberculosis (MTB) growing in a chemostat | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9
The precursor requirements for amino acid pools were derived from measurements of the macromolecular composition of biomass hydrolysates.
Species |  | BCG |  |  |  |  | MTB
intermediate | amino acid | Requirement [µmol gCDW-1] |  | Biomass efflux [mmol gCDW-1 h-1] |  |  | Requirement [µmol gCDW-1] |  | Biomass efflux [mmol gCDW-1 h-1]
 |  | µ = 0.01 h-1 (td = 69 h) | µ = 0.03 h-1 (td = 23 h) | µ = 0.01 h-1 (td = 69 h) (Solution 1) | µ = 0.01 h-1 (td = 69 h) (Solution 2) | µ = 0.03 h-1 (td = 23 h) | µ = 0.01 h-1 (td = 69 h) | µ = 0.01 h-1 (td = 69 h)  (Solution 1) | µ = 0.01 h-1 (td = 69 h) (Solution 2)
G6P |  |  |  | 0.00170153 | 0.00164289 | 0.00608908 |  | 0.00293883 | 0.00319807
F6P |  |  |  | 0.000582026 | 0.000574895 | 0.0020512 |  | 1e-14 | 0.00113851
GA3P |  |  |  | 0.00147822 | 0.00147808 | 0.00500954 |  | 0.000646452 | 0.00175675
R5P1 |  |  |  | 0.00746957 | 0.00685065 | 0.0296062 |  | 0.00906335 | 0.00964754
 | HIS | 0.0 ± 0.0 | 0.8 ± 0.2 | < 10-3 | < 10-3 | < 10-3 | 0.2 ± 0.1 | < 10-3 | < 10-3
E4P |  |  |  | 0.00219696 | 0.00212716 | 0.00765986 |  | 0.00118571 | 0.00235505
PEP2 |  |  |  | 0.00487107 | 0.00464715 | 0.0167035 |  | 0.00615966 | 0.00669334
 | PHE | 0.1 ± 0.0 | 1.2 ± 0.3 | < 10-3 | < 10-3 | 0.00119856 | 0.4 ± 0.1 | < 10-3 | < 10-3
 | TYR | 0.1 ± 0.0 | 0.7 ± 0.1 | < 10-3 | < 10-3 | < 10-3 | 0.3 ± 0.1 | < 10-3 | < 10-3
PGP |  |  |  | 0.0105074 | 0.0097402 | 0.034991 |  | 0.0160292 | 0.0194704
 | SER | 0.0 ± 0.0 | 1.1 ± 0.3 | < 10-3 | < 10-3 | 0.00111534 | 0.3 ± 0.3 | < 10-3 | 0.00143788
 | GLY | 0.1 ± 0.1 | 5.9 ± 1.1 | < 10-3 | < 10-3 | 0.00613473 | 0.9 ± 0.6 | < 10-3 | 0.00267917
PYR3 |  |  |  | 0.021352 | 0.0181945 | 0.0690104 |  | 0.0112718 | 0.0392631
 | ALA | 1.2 ± 0.7 | 15.0 ± 3.6 | 0.00126857 | 0.0011331 | 0.0149549 | 4.2 ± 1.7 | < 10-3 | 0.010746
 | VAL | 0.3 ± 0.1 | 4.0 ± 1.0 | < 10-3 | < 10-3 | 0.00400888 | 1.3 ± 0.4 | < 10-3 | < 10-3
 | LEU | 0.3 ± 0.2 | 5.6 ± 1.6 | < 10-3 | < 10-3 | 0.00592841 | 1.7 ± 0.6 | < 10-3 | < 10-3
ACCOA4
MALOAA |  |  |  | 0.017986 | 0.0138948 | 0.0175685 |  | 0.0246375 | 0.0267717
 | ASP | 0.0 ± 0.0 | 2.9 ± 0.9 | < 10-3 | < 10-3 | 0.00267503 | 0.6 ± 0.6 | < 10-3 | 0.00221868
 | THR | 0.1 ± 0.0 | 2.1 ± 0.3 | < 10-3 | < 10-3 | 0.00207155 | 0.8 ± 0.4 | < 10-3 | 0.00212485
 | ILEU | 0.1 ± 0.1 | 2.2 ± 0.6 | < 10-3 | < 10-3 | 0.00203351 | 0.8 ± 0.2 | < 10-3 | 0.00111066
 | MET | 0.0 ± 0.0 | 0.5 ± 0.1 | < 10-3 | < 10-3 | < 10-3 | 0.2 ± 0.1 | 0.0066413 | < 10-3
 | LYS | 0.1 ± 0.0 | 1.9 ± 1.0 | < 10-3 | < 10-3 | 0.00173118 | 0.5 ± 0.2 | 0.00121784 | < 10-3
OXG |  |  |  | 0.00257575 | 0.00425124 | 0.0343788 |  | 0.000829066 | 0.0017183
 | GLU | 0.0 ± 0.0 | 1.8 ± 0.7 | < 10-3 | < 10-3 | 0.00178133 | 0.4 ± 0.8 | < 10-3 | < 10-3
 | PRO | 0.4 ± 0.2 | n.d. | < 10-3 | < 10-3 | < 10-3 | 1.2 ± 0.5 | < 10-3 | < 10-3
 | ORN | 0.0 ± 0.0 | n.d. | < 10-3 | < 10-3 | < 10-3 | 0.0 ± 0.0 | < 10-3 | < 10-3
[1] R5P is also needed for synthesis of tryptophane (TRP).
2 2 molecules of PEP are needed for synthesis of aromatic amino acids.
3 2 molecules of PYR are needed for synthesis of branched chain amino acids. In addition PYR is needed for synthesis of isoleucine (ILEU) and lysine (LYS).
4 ACCOA is also needed for synthesis of leucine (LEU).