Spreadsheet: 268726d1380756613-help-circumference-error-circumference-error
Sheet: Table 3 Intra

[NAME]
Animal Number | Total | # of Events R1 | # of Events R2 | # of Beads Per Test | Test Volume
MF39741M | 9590 | 7988 | 843 | 50602 | 50
 | Animal No. MF39741M
 | Relative Percentage
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
Replicate 1 | 55 | 9.5 | 16 | 37.5 | 21.8 | 90.1
Replicate 2 | 55.4 | 9.5 | 16 | 38.9 | 21.5 | 90.6
Replicate 3 | 54.8 | 9.4 | 16.4 | 38.1 | 21 | 90.6
Replicate 4 | 55.6 | 10.4 | 16.1 | 40.2 | 21.2 | 91.8
Replicate 5 | 54.9 | 10.3 | 16.6 | 41.5 | 21.7 | 91.9
Replicate 6 | 55.4 | 8.9 | 17.3 | 38.8 | 20.5 | 91.3
Mean | 55.2 | 9.7 | 16.4 | 39.2 | 21.3 | 91.1
SD | 0.3 | 0.6 | 0.5 | 1.5 | 0.5 | 0.7
%CV | 0.5 | 6.2 | 3 | 3.8 | 2.3 | 0.8
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
 | Absolute Counts (Cells/µL)
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
Replicate 1 | 5275 | 501 | 1534 | 575 | 2091 | 1884
Replicate 2 | 5313 | 505 | 1534 | 597 | 2062 | 1868
Replicate 3 | 5255 | 494 | 1573 | 599 | 2014 | 1825
Replicate 4 | 5332 | 555 | 1544 | 621 | 2033 | 1866
Replicate 5 | 5265 | 542 | 1592 | 661 | 2081 | 1912
Replicate 6 | 5313 | 473 | 1659 | 644 | 1966 | 1795
Mean | 5292 | 512 | 1573 | 616 | 2041 | 1858
SD | 31 | 31 | 48 | 32 | 47 | 42
%CV | 1 | 6 | 3 | 5 | 2 | 2
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
 | Mean Fluorescence Intensity of AF488
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
Replicate 1 | 363 | 1278 | 571 | 1049 | 1915 | 2095
Replicate 2 | 367 | 1340 | 539 | 959 | 1947 | 2119
Replicate 3 | 365 | 1319 | 550 | 1008 | 1970 | 2145
Replicate 4 | 374 | 1289 | 587 | 1046 | 2070 | 2230
Replicate 5 | 368 | 1255 | 564 | 979 | 2023 | 2174
Replicate 6 | 360 | 1286 | 553 | 989 | 1984 | 2145
Mean | 366.2 | 1294.5 | 560.7 | 1005 | 1984.8 | 2151.3
SD | 4.8 | 30.3 | 17 | 36.5 | 55.2 | 46.9
%CV | 1.3 | 2.3 | 3 | 3.6 | 2.8 | 2.2
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
[NAME]
Animal Number | Total | # of Events R1 | # of Events R2 | # of Beads Per Test | Test Volume
MF39735M | 9686 | 7743 | 809 | 50602 | 50
 | Animal No. MF39735M
 | Relative Percentage
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
Replicate 1 | 61.1 | 7.1 | 17 | 60.1 | 19.4 | 96.3
Replicate 2 | 60.9 | 6.9 | 17 | 56.4 | 19.9 | 95.7
Replicate 3 | 62.4 | 7.3 | 17 | 57.6 | 18.8 | 96.2
Replicate 4 | 62.4 | 6.8 | 16 | 58.9 | 19.7 | 96.8
Replicate 5 | 62.3 | 7.5 | 16.5 | 59.3 | 19.4 | 95.7
Replicate 6 | 61.3 | 6.6 | 17.5 | 56.9 | 19.2 | 96.4
Mean | 61.7 | 7 | 16.8 | 58.2 | 19.4 | 96.2
SD | 0.7 | 0.3 | 0.5 | 1.5 | 0.4 | 0.4
%CV | 1.1 | 4.3 | 3 | 2.6 | 2.1 | 0.4
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
 | Absolute Counts (Cells/µL)
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
Replicate 1 | 5918 | 420 | 1647 | 990 | 1879 | 1809
Replicate 2 | 5899 | 407 | 1647 | 929 | 1928 | 1845
Replicate 3 | 6044 | 441 | 1647 | 949 | 1821 | 1752
Replicate 4 | 6044 | 411 | 1550 | 913 | 1908 | 1847
Replicate 5 | 6034 | 453 | 1598 | 948 | 1879 | 1798
Replicate 6 | 5938 | 392 | 1695 | 964 | 1860 | 1793
Mean | 5980 | 421 | 1631 | 949 | 1879 | 1807
SD | 68 | 23 | 50 | 27 | 37 | 36
%CV | 1 | 5 | 3 | 3 | 2 | 2
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
 | Mean Fluorescence Intensity of AF488
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
Replicate 1 | 311 | 1222 | 908 | 1299 | 4522 | 4685
Replicate 2 | 307 | 1237 | 854 | 1255 | 4492 | 4684
Replicate 3 | 314 | 1200 | 901 | 1337 | 4520 | 4691
Replicate 4 | 307 | 1214 | 910 | 1318 | 4519 | 4658
Replicate 5 | 321 | 1284 | 883 | 1271 | 4554 | 4748
Replicate 6 | 308 | 1282 | 840 | 1226 | 4371 | 4524
Mean | 311.3 | 1239.8 | 882.7 | 1284.3 | 4496.3 | 4665
SD | 5.5 | 35.5 | 29.6 | 41.4 | 64.5 | 75.2
%CV | 1.8 | 2.9 | 3.4 | 3.2 | 1.4 | 1.6
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass