Spreadsheet: 10633c66-f93c-406d-90b0-64d73590ac29
Sheet: TableS8-ParametersForModel

 |  |  |  | Growth  measurements used in the model
 |  |  |  |  | Specific exponential growth rate per hour*
 |  |  |  |  | glucose |  | maltose |  | Lag distributions in maltose, in hours*** | Percent growing within 24 hours.
 |  |  |  |  | average (n=5) | sd | average (n=5) | sd
 |  |  |  | Isolate 1 | 0.352** | 0.003160632 | 0.329020139 | 0.006683562 | rnorm(50000,mean(SCLag****),sd(SCLag)) | 99.4% divide within 24 hours
 |  |  |  | Isolate 6 | 0.448969418 | 0.006365841 | 0.31071292 | 0.007706673 | runif(50000)*16+7.5***** | 4.95% divide within 24 hours
 |  |  |  |  | * These growth rates were determined for cultures that had adapted to identical media as that used in the glucose>maltose switching experiments. Cells were adapted for 8 hours in glucose or maltose media (growth here in a 96-well plate shaking in an orbital plate shaker), diluted to approximately 1-2000 cells into 5 ml of pre-warmed media identical to that which they had adpated, and 100 ul of this culture was then spread out on YPD agar plates such that  ~1-200 single colonies would develop within two days of incubation at 30 C. The cultures were placed on a roller wheel at 30 degrees and allowed to grow for an additional 12 to 16 hours. After this period of time, cells from these cultures were  diluted to ~1-2000 cells / ml, and 100 ul of this cell suspension was again spread out on YPD agar plates and allowed to develop to single colonies. These growth rates represent the ln(CellDensFinal/CellDensInitial)/deltatime .
 |  |  |  |  | ** In the cultures cycling between 12 hours of growth in maltose followed by 12 hours of growth in glucose, we found that Isolate 1 grew 0.701 +/- 0.007 times more slowly than Isolate 6, about 10% more slowly than when both populations are adapted to glucose as measured above. Therefore, for this regime of environmental change, we used a growth rate of 0.315 per hour as a growth rate for the glucose leg of the growth for Isolate 1.
 |  |  |  |  | *** Lag distributions in maltose media were determined for these strains after 20 hours of growth in glucose as in Figure 3a. These measurements were used to obtain lag phase distributions using a random normal distribution (R function rnorm) or a uniform distribution (R  function runif).
 |  |  |  |  | **** SCLag = the vector of single-cell lag times measured for this strain, representing 175 single-cell measurements. Cells drawn from the normal distribution were scored by the model to have a lag phase = 0 by the model.
 |  |  |  |  | ***** This random uniform distribution combined with cell statuses provided a close match to that observed for the 2,104 single-cell observations of Isolate 6.
 |  |  |  | List of empirically measured lag phases
 |  |  |  | Isolate 1 Lag time (hours) | status(Isolate1) | Isolate 6 Lag time (hours) | Status (Isolate 6) |  | Status of 1 means the cell was observed to grow. If the cell did not begin growth, its status was = 0 and the latest time point of the movie was recorded.
 |  |  |  | 2.337333 | 1 | 4.741 | 1
 |  |  |  | 9.228333 | 1 | 7.265 | 1
 |  |  |  | 10.543333 | 1 | 7.692 | 1
 |  |  |  | 5.946333 | 1 | 7.769 | 1
 |  |  |  | 3.980333 | 1 | 8.013 | 1
 |  |  |  | 4.960333 | 1 | 8.013 | 1
 |  |  |  | 2.667333 | 1 | 9.006 | 1
 |  |  |  | 5.618333 | 1 | 9.48 | 1
 |  |  |  | 8.900333 | 1 | 9.498 | 1
 |  |  |  | 3.980333 | 1 | 9.514 | 1
 |  |  |  | 10.543333 | 1 | 9.515 | 1
 |  |  |  | 3.652333 | 1 | 9.709 | 1
 |  |  |  | 8.903333 | 1 | 9.722 | 1
 |  |  |  | 8.903333 | 1 | 10.221 | 1
 |  |  |  | 10.218333 | 1 | 10.221 | 1
 |  |  |  | 8.573333 | 1 | 10.741 | 1
 |  |  |  | 9.222333 | 1 | 10.753 | 1
 |  |  |  | 2.998333 | 1 | 10.76 | 1
 |  |  |  | 3.327333 | 1 | 11.227 | 1
 |  |  |  | 2.013333 | 1 | 11.454 | 1
 |  |  |  | 8.903333 | 1 | 11.466 | 1
 |  |  |  | 4.314333 | 1 | 11.489 | 1
 |  |  |  | 3.987333 | 1 | 11.5 | 1
 |  |  |  | 3.002333 | 1 | 11.705 | 1
 |  |  |  | 3.002333 | 1 | 11.764 | 1
 |  |  |  | 2.344333 | 1 | 12.496 | 1
 |  |  |  | 0.333333 | 1 | 12.523 | 1
 |  |  |  | 3.987333 | 1 | 12.527 | 1
 |  |  |  | 2.344333 | 1 | 12.74 | 1
 |  |  |  | 7.264333 | 1 | 12.764 | 1
 |  |  |  | 13.185333 | 1 | 13.002 | 1
 |  |  |  | 5.625333 | 1 | 13.192 | 1
 |  |  |  | 3.002333 | 1 | 13.211 | 1
 |  |  |  | 5.954333 | 1 | 13.249 | 1
 |  |  |  | 10.880333 | 1 | 13.461 | 1
 |  |  |  | 6.941333 | 1 | 13.739 | 1
 |  |  |  | 9.239333 | 1 | 13.759 | 1
 |  |  |  | 3.662333 | 1 | 14.018 | 1
 |  |  |  | 15.519333 | 1 | 14.206 | 1
 |  |  |  | 4.645333 | 1 | 14.476 | 1
 |  |  |  | 4.317333 | 1 | 14.498 | 1
 |  |  |  | 7.595933 | 1 | 14.501 | 1
 |  |  |  | 10.554333 | 1 | 14.971 | 1
 |  |  |  | 3.335333 | 1 | 15.024 | 1
 |  |  |  | 2.348333 | 1 | 15.189 | 1
 |  |  |  | 3.662333 | 1 | 15.216 | 1
 |  |  |  | 3.990333 | 1 | 15.445 | 1
 |  |  |  | 4.645333 | 1 | 15.479 | 1
 |  |  |  | 10.225333 | 1 | 15.745 | 1
 |  |  |  | 15.519333 | 1 | 15.769 | 1
 |  |  |  | 9.568333 | 1 | 15.992 | 1
 |  |  |  | 3.335333 | 1 | 16.02 | 1
 |  |  |  | 7.595333 | 1 | 16.219 | 1
 |  |  |  | 4.972333 | 1 | 16.265 | 1
 |  |  |  | 2.685333 | 1 | 16.267 | 1
 |  |  |  | 4.324333 | 1 | 16.515 | 1
 |  |  |  | 3.013333 | 1 | 16.762 | 1
 |  |  |  | 3.998333 | 1 | 16.976 | 1
 |  |  |  | 3.670333 | 1 | 17.195 | 1
 |  |  |  | 8.260333 | 1 | 17.231 | 1
 |  |  |  | 9.247333 | 1 | 17.485 | 1
 |  |  |  | 11.549333 | 1 | 17.506 | 1
 |  |  |  | 4.652333 | 1 | 17.711 | 1
 |  |  |  | 8.260333 | 1 | 17.759 | 1
 |  |  |  | 5.636333 | 1 | 17.763 | 1
 |  |  |  | 10.232333 | 1 | 17.957 | 1
 |  |  |  | 4.324333 | 1 | 17.977 | 1
 |  |  |  | 4.983333 | 1 | 17.977 | 1
 |  |  |  | 6.624333 | 1 | 17.979 | 1
 |  |  |  | 6.952333 | 1 | 18.004 | 1
 |  |  |  | 5.310333 | 1 | 18.241 | 1
 |  |  |  | 25 | 0 | 18.739 | 1
 |  |  |  | 4.983333 | 1 | 18.962 | 1
 |  |  |  | 3.345333 | 1 | 19.184 | 1
 |  |  |  | 6.296333 | 1 | 19.254 | 1
 |  |  |  | 9.579333 | 1 | 19.46 | 1
 |  |  |  | 12.211333 | 1 | 19.505 | 1
 |  |  |  | 4.983333 | 1 | 19.742 | 1
 |  |  |  | 3.673333 | 1 | 19.742 | 1
 |  |  |  | 5.310333 | 1 | 20.235 | 1
 |  |  |  | 8.264333 | 1 | 20.462 | 1
 |  |  |  | 2.359333 | 1 | 20.933 | 1
 |  |  |  | 2.359333 | 1 | 21.19 | 1
 |  |  |  | 3.017333 | 1 | 21.215 | 1
 |  |  |  | 3.345333 | 1 | 21.228 | 1
 |  |  |  | 4.983333 | 1 | 21.244 | 1
 |  |  |  | 8.264333 | 1 | 21.263 | 1
 |  |  |  | 3.676333 | 1 | 21.443 | 1
 |  |  |  | 8.268333 | 1 | 21.491 | 1
 |  |  |  | 9.254333 | 1 | 22.007 | 1
 |  |  |  | 4.005333 | 1 | 22.247 | 1
 |  |  |  | 3.349333 | 1 | 22.408 | 1
 |  |  |  | 8.268333 | 1 | 22.456 | 1
 |  |  |  | 8.268333 | 1 | 22.758 | 1
 |  |  |  | 2.035333 | 1 | 22.964 | 1
 |  |  |  | 6.300333 | 1 | 22.992 | 1
 |  |  |  | 3.021333 | 1 | 22.994 | 1
 |  |  |  | 5.314333 | 1 | 23.002 | 1
 |  |  |  | 6.300333 | 1 | 23.505 | 1
 |  |  |  | 13.203333 | 1 | 24.277 | 1
 |  |  |  | 7.610333 | 1 | 24.48 | 1
 |  |  |  | 5.647333 | 1 | 24.499 | 1
 |  |  |  | 3.353333 | 1 | 25.507 | 1
 |  |  |  | 3.353333 | 1 | 25.507 | 1
 |  |  |  | 1.709333 | 1 | 26 | 0
 |  |  |  | 3.353333 | 1 | 26 | 0
 |  |  |  | 6.303333 | 1 | 26 | 0
 |  |  |  | 4.335333 | 1 | 26 | 0
 |  |  |  | 2.696333 | 1 | 26 | 0
 |  |  |  | 5.647333 | 1 | 26 | 0
 |  |  |  | 5.318333 | 1 | 26 | 0
 |  |  |  | 0.333333 | 1 | 26 | 0
 |  |  |  | 4.008333 | 1 | 26 | 0
 |  |  |  | 3.353333 | 1 | 26 | 0
 |  |  |  | 4.008333 | 1 | 26 | 0
 |  |  |  | 7.613333 | 1 | 26 | 0
 |  |  |  | 6.303333 | 1 | 26 | 0
 |  |  |  | 10.902333 | 1 | 26 | 0
 |  |  |  | 4.663333 | 1 | 26 | 0
 |  |  |  | 3.024333 | 1 | 26 | 0
 |  |  |  | 5.318333 | 1 | 26 | 0
 |  |  |  | 5.318333 | 1 | 26 | 0
 |  |  |  | 3.032333 | 1 | 26 | 0
 |  |  |  | 7.949333 | 1 | 26 | 0
 |  |  |  | 4.670333 | 1 | 26 | 0
 |  |  |  | 5.983333 | 1 | 26 | 0
 |  |  |  | 6.967333 | 1 | 26 | 0
 |  |  |  | 12.554333 | 1 | 26 | 0
 |  |  |  | 3.032333 | 1 | 26 | 0
 |  |  |  | 4.016333 | 1 | 26 | 0
 |  |  |  | 4.343333 | 1 | 26 | 0
 |  |  |  | 5.654333 | 1 | 26 | 0
 |  |  |  | 4.997333 | 1 | 26 | 0
 |  |  |  | 4.343333 | 1 | 26 | 0
 |  |  |  | 7.949333 | 1 | 26 | 0
 |  |  |  | 3.032333 | 1 | 26 | 0
 |  |  |  | 4.670333 | 1 | 26 | 0
 |  |  |  | 8.278333 | 1 | 26 | 0
 |  |  |  | 9.953333 | 1 | 26 | 0
 |  |  |  | 4.016333 | 1 | 26 | 0
 |  |  |  | 4.343333 | 1 | 26 | 0
 |  |  |  | 3.691333 | 1 | 26 | 0
 |  |  |  | 5.658333 | 1 | 26 | 0
 |  |  |  | 5.987333 | 1 | 26 | 0
 |  |  |  | 3.364333 | 1 | 26 | 0
 |  |  |  | 21.364333 | 1 | 26 | 0
 |  |  |  | 5.329333 | 1 | 26 | 0
 |  |  |  | 8.940333 | 1 | 26 | 0
 |  |  |  | 0.333333 | 1 | 26 | 0
 |  |  |  | 3.364333 | 1 | 26 | 0
 |  |  |  | 2.050333 | 1 | 26 | 0
 |  |  |  | 6.314333 | 1 | 26 | 0
 |  |  |  | 4.346333 | 1 | 26 | 0
 |  |  |  | 3.691333 | 1 | 26 | 0
 |  |  |  | 0.333333 | 1 | 26 | 0
 |  |  |  | 4.674333 | 1 | 26 | 0
 |  |  |  | 4.346333 | 1 | 26 | 0
 |  |  |  | 5.658333 | 1 | 26 | 0
 |  |  |  | 6.314333 | 1 | 26 | 0
 |  |  |  | 5.662333 | 1 | 26 | 0
 |  |  |  | 7.628333 | 1 | 26 | 0
 |  |  |  | 8.943333 | 1 | 26 | 0
 |  |  |  | 5.005333 | 1 | 26 | 0
 |  |  |  | 11.575333 | 1 | 26 | 0
 |  |  |  | 2.711333 | 1 | 26 | 0
 |  |  |  | 4.678333 | 1 | 26 | 0
 |  |  |  | 5.990333 | 1 | 26 | 0
 |  |  |  | 9.601333 | 1 | 26 | 0
 |  |  |  | 9.928333 | 1 | 26 | 0
 |  |  |  | 5.662333 | 1 | 26 | 0
 |  |  |  | 9.272333 | 1 | 26 | 0
 |  |  |  | 7.628333 | 1 | 26 | 0
 |  |  |  | 5.333333 | 1 | 26 | 0
 |  |  |  | 7.956333 | 1 | 26 | 0
 |  |  |  | 6.318333 | 1 | 26 | 0
 |  |  |  |  |  | 26 | 0
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