Spreadsheet: a031fb64-b26b-4f2b-9716-49383c25c11a
Sheet: Sheet1

Supplementary Table 9: Details of the 93 protein families that may be unique to microsporidia including representation in the spore proteomics data. * denotes ORF that is present in the spore proteomics data. Min_tm and max_tm give the smallest and largest number of transmembrane domains found in proteins of the respective family. Signal peptide predictions (SignalP) are based on T. hominis only. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11
T.hominis ORF(s) | Best KOG hits based on BLASTP  (cut-off e-value ≤ 0.01) | Gene product annotation | No. of Thom genes in protein cluster | No. of Nce genes in protein cluster | No. of Ebi genes in protein cluster | No. of Ecu genes in protein cluster | No. of Ein genes in protein cluster | Putative T.hominis transporters | min_tm | max_tm | signalp
orf_3144 | Within-Microsporidia Blast hits only | Projectin/twitchin and related proteins | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | 26-26
orf_2528 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | 24-24
orf_1365 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2810 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1070 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_916 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 2 | 1 | 1 |  | 0 | 0 | no_sigp
orf_117, orf_2882 | Within-Microsporidia Blast hits only | uncharacterised conserved transporter | 2 | 1 | 0 | 1 | 1 | yes | 4 | 5 | no_sigp
orf_1554* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_500 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 1 | 1 | no_sigp
orf_391 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2505 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_314 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_871 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_600 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_768 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_567 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1530 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 1 | 1 | 17-22
orf_144 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_169 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1334, orf_1382, orf_150, orf_257, orf_3244, orf_51, orf_52, orf_53 | Within-Microsporidia Blast hits only | uncharacterised conserved transporter | 8 | 0 | 0 | 2 | 2 | yes | 2 | 4 | 25-59
orf_368 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 10 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1576* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 15 | 1 | 1 |  | 0 | 0 | 18-22
orf_2624 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 0 | 0 |  | 0 | 0 | no_sigp
orf_1745* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 0 | 0 |  | 1 | 2 | 20-20
orf_2016 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 0 | 0 |  | 0 | 0 | no_sigp
orf_505 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1869 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_3259 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_105 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_3088 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_210 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_3208 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_972 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2990 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_742* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 0 | 1 |  | 1 | 1 | 22-24
orf_2301 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2189 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2020 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_397 | Within-Microsporidia Blast hits only | Extracellular matrix glycoprotein Laminin subunits alpha and gamma | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | 26-26
orf_1364* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | 17-22
orf_1779 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2871 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1109 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 1 | 1 |  | 0 | 1 | no_sigp
orf_2275 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2656 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2827 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | 22-22
orf_1783 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_185 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1785 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1145 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2732 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 1 | 16-17
orf_2446 | Within-Microsporidia Blast hits only | uncharacterised conserved transporter | 1 | 1 | 0 | 1 | 1 | yes | 3 | 4 | no_sigp
orf_2196 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | 25-25
orf_2052 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2825 | Within-Microsporidia Blast hits only | protein containing Zinc finger, [NAME]-like | 1 | 1 | 0 | 1 | 1 |  | 0 | 1 | no_sigp
orf_1274 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | 18-23
orf_116 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2878 | Within-Microsporidia Blast hits only | uncharacterised conserved transporter | 1 | 1 | 0 | 1 | 1 | yes | 5 | 7 | 28-37
orf_1333 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2494 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 2 | 7 | 33-43
orf_5 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 1 | no_sigp
orf_44 | Within-Microsporidia Blast hits only | uncharacterised conserved transporter | 1 | 1 | 1 | 1 | 1 | yes | 2 | 5 | 25-25
orf_1106 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | 31-31
orf_3112 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2356 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 1 | 3 | no_sigp
orf_1756* | Within-Microsporidia Blast hits only | putative polar tube protein 2 (PTP2) | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | 16-24
orf_2515 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_3203 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_699 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_686* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 4 | 4 | no_sigp
orf_2900 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2172* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 1 | 18-25
orf_152* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_417 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_248 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2715, orf_2716 | Within-Microsporidia Blast hits only | uncharacterised conserved transporter | 2 | 1 | 0 | 1 | 1 | yes | 6 | 8 | 23-24
orf_1343, orf_1361* | Within-Microsporidia Blast hits only | hypothetical protein | 2 | 5 | 0 | 0 | 0 |  | 0 | 1 | 17-19
orf_1575* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 2 | 0 | 2 | 2 |  | 0 | 0 | 17-30
orf_1838*, orf_1840*, orf_1843*, orf_1643* | Within-Microsporidia Blast hits only | hypothetical protein, orf_1643 - protein containing Ricin B-related lectin | 4 | 0 | 0 | 2 | 2 |  | 0 | 0 | 15-27
orf_2110*, orf_2994*, orf_3181* | Within-Microsporidia Blast hits only | hypothetical protein | 3 | 2 | 1 | 1 | 1 |  | 0 | 1 | 18-24
orf_2317 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2632* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2037 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2971* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1979 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1287 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_2002* | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp
orf_956 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_1359 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 0 | 0 |  | 0 | 0 | no_sigp
orf_1909 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 0 | 1 | 1 |  | 0 | 0 | no_sigp
orf_543 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 2 | 2 | 29-32
orf_3050 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 0 | 0 | 1 | 1 |  | 0 | 1 | 17-24
orf_777 | Within-Microsporidia Blast hits only | hypothetical protein | 1 | 1 | 1 | 1 | 1 |  | 0 | 0 | no_sigp