Spreadsheet: 912a7455-7864-48bf-ae09-273c8521b691
Sheet: Sheet1

Table S2. Suppressors of GAL-MPS3-G186K. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4
ORF name | Protein* | Standard Name | Alias | Description
YAL013W | YES | DEP1 | FUN54 | Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation
YAR002W | YES | NUP60 |  | Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; involved in nuclear export and cytoplasmic localization of specific mRNAs such as ASH1
YBL079W | YES | NUP170 | NLE3 | Subunit of the nuclear pore complex (NPC), required for NPC localization of specific nucleoporins; involved in nuclear envelope permeability and chromosome segregation; has similar to Nup157p; essential role, with Nup157p, in NPC assembly
YBR139W | YES |  |  | Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
YDL005C | YES | MED2 |  | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YDL160C | YES | DHH1 |  | Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping, coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation
YDR290W | YES |  | RTT103 | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
YDR532C | YES | KRE28 |  | Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components
YER106W | YES | [NAME] |  | Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle
YER156C | YES |  |  | Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for [NAME], a copper-responsive transcription factor; similarity to mammalian MYG1
YGL025C | YES | PGD1 | HRS1 , [NAME] | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YHL029C | YES | OCA5 |  | Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
YHR121W | YES | LSM12 |  | Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS
YIL009W | YES | FAA3 |  | Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YIL154C | YES | IMP2' | IMP2 | Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YKL160W | YES | ELF1 |  | Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
YKR103W | YES | [NAME] |  | Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds
YLR018C | YES | [NAME] |  | Integral membrane protein of the nuclear pore; has an important role in maintaining the architecture of the pore complex
YLR200W | YES | YKE2 | GIM1 , PFD6 | Subunit of the heterohexameric [NAME]/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
[NAME] | YES | SIR3 | CMT1, [NAME], STE8 | Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p
YML010W-A | YES |  |  | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition (deletion of C-terminus of essential SPT5 gene)
YML103C | YES | NUP188 |  | Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with [NAME] and Nic96p
YMR066W | YES | SOV1 |  | Mitochondrial protein of unknown function
YMR119W | YES | ASI1 |  | Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and [NAME] to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
YMR129W | YES | POM152 |  | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase
YMR153W | YES | NUP53 |  | Subunit of the nuclear pore complex (NPC), interacts with karyopherin [NAME] or with [NAME] via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex
YMR266W | YES | RSN1 |  | Membrane protein of unknown function; overexpression suppresses [NAME] sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
YNL133C | YES | FYV6 |  | Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
YNL288W | YES | CAF40 |  | Evolutionarily conserved subunit of the [NAME]-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
[NAME] | YES | AGA1 |  | Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
YOR083W | YES | WHI5 |  | Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
YOR141C | YES | ARP8 |  | Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YPL161C | YES | BEM4 | ROM7 | Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YPL183W-A | YES | RTC6 | TAE4 | [NAME] of the prokaryotic ribosomal protein [NAME], likely to be a mitochondrial ribosomal protein coded in the nuclear genome; null mutation suppresses cdc13-1 temperature sensitivity
YBR246W | TRUNC | RRT2 |  | Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; null mutants display a weak carboxypeptidase Y missorting/secretion phenotype
YAL017W | NO | PSK1 | FUN31 | One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status
YAR035W | NO | YAT1 |  | Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
YBR206W | NO |  |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3
YBR231C | NO | SWC5 | AOR1 | Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YBR233W | NO | PBP2 | HEK1 | RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length
[NAME] | NO |  |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; [NAME] is not an essential gene
YDR133C | NO |  |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
YDR142C | NO | PEX7 | PAS7  PEB1 | Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
YDR195W | NO | REF2 |  | RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes
[NAME] | NO | MNN10 | BED1  SLC2  REC41 | Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, [NAME], and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
YER119C-A | NO |  |  | Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YGL057C | NO | GEP7 |  | Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin ([NAME]) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL180W | NO | ATG1 | AUT3, CVT10, APG1 | Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and [NAME]
YGL216W | NO | KIP3 |  | Kinesin-related motor protein involved in mitotic spindle positioning
YGL231C | NO | EMC4 |  | Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; human ortholog TMEM85 may function in apoptosis
YGL257C | NO | MNT2 |  | Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans
YGR217W | NO | CCH1 |  | Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
YGR289C | NO | MAL11 | AGT1 | Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
[NAME] | NO | PTC7 |  | Type 2C protein phosphatase; [NAME] is alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope
YIL110W | NO | HPM1 | MNI1 | AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
[NAME] | NO | [NAME] |  | Protein of unknown function
YJR055W | NO | HIT1 |  | Protein of unknown function, required for growth at high temperature
YKL137W | NO | CMC1 |  | Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif
YLR247C | NO | IRC20 |  | Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci
YLR404W | NO | FLD1 |  | Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
[NAME] | NO | BUL2 |  | Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1
YNL072W | NO | RNH201 | [NAME] | [NAME] catalytic subunit, removes RNA primers during Okazaki fragment synthesis; homolog of RNAse HI (the S. cerevisiae homolog of mammalian RNAse HII is [NAME]); related to human [NAME] that causes [NAME] syndrome
[NAME] | NO | YNG1 | YOR29-15 | Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1
[NAME] | NO | IES4 |  | Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses
YOR233W | NO | KIN4 | KIN31 , [NAME] | Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck
YPL008W | NO | CHL1 | [NAME], LPA9, MCM12 | Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor [NAME]; associates with acetyltransferase [NAME]
YPL037C | NO | EGD1 |  | [NAME] of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
YPL054W | NO | LEE1 |  | Zinc-finger protein of unknown function
YPL069C | NO | BTS1 |  | Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YPL101W | NO | ELP4 | HAP1 , [NAME],  KTI9 | Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
YPL145C | NO | KES1 | LPI3 , OSH4, BSR3 | Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex
YPL248C | NO | GAL4 | GAL81 | DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p
YPR038W | NO | IRC16 |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci
YBL027W | LOW | RPL19B |  | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal
YBR156C | LOW | [NAME] |  | Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly
[NAME] | LOW | RPS11A |  | Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins
YER117W | LOW | RPL23B |  | Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E. coli [NAME] and rat [NAME] ribosomal proteins
YFR055W | LOW | [NAME] |  | Putative cystathionine beta-lyase; involved in copper ion homeostasis and sulfur metabolism; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner
YGR063C | LOW | SPT4 |  | Protein involved in the regulating [NAME] I and Pol II transcription, pre-mRNA processing, kinetochore function, and gene silencing; forms a complex with Spt5p
YHL019C | LOW | APM2 |  | Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
[NAME] | LOW | RPL2B | RPL5A , LOT2 | Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures
[NAME] | LOW |  |  | Putative protein of unknown function
YIL103W | LOW | [NAME] |  | Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
[NAME] | LOW | MET18 | MMS19 | DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; involved in telomere maintenance
YIR009W | LOW | MSL1 | [NAME] | U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
[NAME] | LOW | GAL2 |  | Galactose permease, required for utilization of galactose; also able to transport glucose
YML014W | LOW | TRM9 | KTI1 | tRNA methyltransferase, catalyzes esterification of modified uridine nucleotides in tRNA(Arg3) and tRNA(Glu), likely as part of a complex with Trm112p; deletion confers resistance to zymocin
YML026C | LOW | RPS18B |  | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins
YMR224C | LOW | MRE11 | RAD58 , XRS4 , [NAME] | Subunit of a complex with Rad50p and Xrs2p (MRX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for MRX function
YOL051W | LOW | GAL11 | SPT13, [NAME], MED15, RAR3 , [NAME] | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors
YOR100C | LOW | CRC1 |  | Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation
* YES indicates that full-length Mps3-G186K was expressed in the mutant at levels equivalent to wild-type control cells, NO indicates that no expression of the dominant protein was observed, TRUNC indicates that a truncated version of Mps3-G186K was observed, and LOW indicates that a reduced levels of the mutant protein was observed.