Spreadsheet: 81a6cd3c-ca39-4faa-80cc-c29297550bd8
Sheet: Sheet1

Table S2: Primer sequences used in this study | Unnamed: 1 | Unnamed: 2 | Unnamed: 3
Transcription start site mapping:
Name | Forward | Reverse
Angptl4 5’RACE F | GAGAGTGCTGCGACAGCTAA
Angptl4 5’RACE R2 |  | GCAGCTCCTCTCCTCTCCAT
Angptl4 5’RACE R1 |  | ATCATCCCACGCTGCATATT
Promoter reporter analysis:
Name | Forward | Reverse | Cloning Method
cfos | TAATCTCGAGGTTTAAACCCAGTGACGTAGGAAGTCCA | TAATGGATCCCGAAGTTTGGGGAAAGCCCG | BamHI/XhoI
Dr [NAME] | TAATCTCGAGGCGATATTTTACTGTTCTGC | TAATGGATCCCTTGCTTGTTTTGTGTCTGTAG | BamHI/XhoI
Dr -3.5kb | TAATCTCGAGCTTCTGTGATGCAGATGTTGTG | TAATGGATCCCTTGCTTGTTTTGTGTCTGTAG | BamHI/XhoI
Dr -1kb | TAATCTCGAGTCCTGGCTTCTGCTCCACTTAC | TAATGGATCCCTTGCTTGTTTTGTGTCTGTAG | BamHI/XhoI
Truncation mapping:
Name | Forward | Reverse | Cloning Method
Dr in3 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTATAAGTCAGTTTATCATT | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recombination
Dr in3.1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTATAAGTCAGTTTATCATT | GGGGACCACTTTGTACAAGAAAGCTGGGTGAATCAGAGCCTTCATTACTTGA | Gateway Recombination
Dr in3.2 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTCAGTTAATGTAGGGCATCC | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recombination
Dr in3.3 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTCAGTTAATGTAGGGCATCC | GGGGACCACTTTGTACAAGAAAGCTGGGTGGTGTGTTTGGTTAAACATCTGC | Gateway Recombination
Dr in3.4 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCTTGTAGGCTGTTGGAAATAC | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recombination
Dr in3.5 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCTTGTAGGCTGTTGGAAATAC | GGGGACCACTTTGTACAAGAAAGCTGGGTGCAAATGACCTTTGCCCAAG | Gateway Recombination
Dr in3.6 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCTTGTAGGCTGTTGGAAATAC | GGGGACCACTTTGTACAAGAAAGCTGGGTCTGTACCCAAACAAAGATGT | Gateway Recombination
Dr in3.7 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCTTGTAGGCTGTTGGAAATAC | GGGGACCACTTTGTACAAGAAAGCTGGGTTTGAACATGTCACCCAAGAACC | Gateway Recombination
Dr in3.8 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGGTCCAGTGTTTGAGATAAG | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recombination
Dr in3.9 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACTCCATGCTGGCTTCTGGG | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recombination
Dr in3.10 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTTTAATAACAACACTTCAG | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recombination
Dr in3.11 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACTCCATGCTGGCTTCTGGG | GGGGACCACTTTGTACAAGAAAGCTGGGTGCAAATGACCTTTGCCCAAG | Gateway Recombination
Dr in3.12 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACTCCATGCTGGCTTCTGGG | GGGGACCACTTTGTACAAGAAAGCTGGGTAACGCTCACACGTCCAGGAT | Gateway Recombination
Dr in3.13 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTCAGTTAATGTAGGGCATCC | GGGGACCACTTTGTACAAGAAAGCTGGGTGAGGAAAGTCTCTGCTTGGG | Gateway Recombination
Dr in3.14 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTCAGTTAATGTAGGGCATCC | GGGGACCACTTTGTACAAGAAAGCTGGGTGGGAGGAAAGAATGCTTGTA | Gateway Recombination
Dr in3.15 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTGGAGAGCCGGACTAAAGG | GGGGACCACTTTGTACAAGAAAGCTGGGTGGTGTGTTTGGTTAAACATCTGC | Gateway Recombination
Dr in3.16 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACCAGCAGCCACGGCATTCC | GGGGACCACTTTGTACAAGAAAGCTGGGTGGTGTGTTTGGTTAAACATCTGC | Gateway Recombination
Dr in3.17 | GGGGACAAGTTTGTACAAAAAAGCAGGCTACCAGCAGCCACGGCATTCC | GGGGACCACTTTGTACAAGAAAGCTGGGTGAGGAAAGTCTCTGCTTGGG | Gateway Recombination
in3.4_ds-inv | TAATAGATCTGGCCGGCCCCTTGTAGGCTGTTGGAAAT | TAATAGATCTGGCGCGCCCTGAAAGACACAAACACATG | BglII
Site-directed mutagenesis:
Name | Forward | Reverse | Cloning Method
Sub1 | ACCCTGGAGTTCAAAACAAAAGATCGATGTGCTTCTGGGTTCTTGGGTGA | TCACCCAAGAACCCAGAAGCACATCGATCTTTTGTTTTGAACTCCAGGGT | Circular PCR/Dpn1/ClaI
Sub2 | CAAAACAAACTCCATGCTGGAAATCGATGGCTTGGGTGACATGTTCAAGG | CCTTGAACATGTCACCCAAGCCATCGATTTCCAGCATGGAGTTTGTTTTG | Circular PCR/Dpn1/ClaI
Sub3 | [NAME] | GTGACAAACCGAACCCAGAAGCCAGCATGG | Linker mediated PCR
Sub4 | TCTGGGTTCTTGGGTGACATTAATCGATGACAGTGTTTGAGATAAGGATT | AATCCTTATCTCAAACACTGTCATCGATTAATGTCACCCAAGAACCCAGA | Circular PCR/Dpn1/ClaI
Sub5 | TGGGTGACATGTTCAAGGTCACATCGATTCGATAAGGATTTAGTACACCA | TGGTGTACTAAATCCTTATCGAATCGATGTGACCTTGAACATGTCACCCA | Circular PCR/Dpn1/ClaI
Sub6 | [NAME] | ATTGTTTAATGGTGTACTAACCATCGATTGCAAACACTGGACCTTGAACA | Circular PCR/Dpn1/ClaI
Sub7 | CCAGTGTTTGAGATAAGGATGCATCGATAAATTAAACAATAAGATAAACA | TGTTTATCTTATTGTTTAATTTATCGATGCATCCTTATCTCAAACACTGG | Circular PCR/Dpn1/ClaI
Sub8 | GATAAGGATTTAGTACACCAGAATCGATGCAGATAAACACTTATCCTGGA | TCCAGGATAAGTGTTTATCTGCATCGATTCTGGTGTACTAAATCCTTATC | Circular PCR/Dpn1/ClaI
Sub9 | TAGTACACCATTAAACAATACAATCGATGGTTATCCTGGACGTGTGAGCG | CGCTCACACGTCCAGGATAACCATCGATTGTATTGTTTAATGGTGTACTA | Circular PCR/Dpn1/ClaI
Sub10 | TAAACAATAAGATAAACACTGCATCGATGAGTGTGAGCGTTTTAAATACT | AGTATTTAAAACGCTCACACTCATCGATGCAGTGTTTATCTTATTGTTTA | Circular PCR/Dpn1/ClaI
Sub11 | ATAAACACTTATCCTGGACGGTATCGATGGTTAAATACTTTGGCAACTTT | AAAGTTGCCAAAGTATTTAACCATCGATACCGTCCAGGATAAGTGTTTAT | Circular PCR/Dpn1/ClaI
Sub12 | ATCCTGGACGTGTGAGCGTTGCATCGATGGTGGCAACTTTAACATCTTTG | CAAAGATGTTAAAGTTGCCACCATCGATGCAACGCTCACACGTCCAGGAT | Circular PCR/Dpn1/ClaI
Sub13 | TGTGAGCGTTTTAAATACTTGTATCGATGGAACATCTTTGTTTGGGTACA | TGTACCCAAACAAAGATGTTCCATCGATACAAGTATTTAAAACGCTCACA | Circular PCR/Dpn1/ClaI
Sub14 | TTAAATACTTTGGCAACTTTCCATCGATGTTTTGGGTACAGCCTTGGGCA | TGCCCAAGGCTGTACCCAAAACATCGATGGAAAGTTGCCAAAGTATTTAA | Circular PCR/Dpn1/ClaI
Sub15 | TGGCAACTTTAACATCTTTGGCATCGATACGCCTTGGGCAAAGGTCATTT | AAATGACCTTTGCCCAAGGCGTATCGATGCCAAAGATGTTAAAGTTGCCA | Circular PCR/Dpn1/ClaI
Sub16 | AACATCTTTGTTTGGGTACATAATCGATACAAGGTCATTTGCAGATGCTT | AAGCATCTGCAAATGACCTTGTATCGATTATGTACCCAAACAAAGATGTT | Circular PCR/Dpn1/ClaI
Sub17 | TTTGGGTACAGCCTTGGGCACCATCGATGGGCAGATGCTTGAACATGTGT | ACACATGTTCAAGCATCTGCCCATCGATGGTGCCCAAGGCTGTACCCAAA | Circular PCR/Dpn1/ClaI
Angptl4 intron 3 cloning for sequencing from multiple species:
Name | Forward | Reverse | Cloning Method
Danio_angptl4_ex3-4 | CTGAACGCGAGCACTGAACAGCGAG | AACAACTCATGACAGTCAGAAGCCA | Topo TA
Cyprinoforme_anpgptl4_ex3-4 | CTGAACGCGAGCTCTGAACAGCGAG | AACAGCTCATGACAGTCAGAAGCCA | Topo TA
Ip_angptl4_ex3-4 | CCCGAGTGCTGATGAAGTTC | TCTCTCCACGTAGGAACACA | Topo TA
Danio_in3.2_F | GTCAGTTAATGTAGGGCATCC | AACAACTCATGACAGTCAGAAGCCA | Topo TA
Intron 3 for reporter assay from multiple species:
Name | Forward | Reverse | Cloning Method
Cyprinoforme_in3.2 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGTCAGTTAATGTAGGGCATCC | GGGGACCACTTTGTACAAGAAAGCTGGGTACTGAAAGACACAAACACA | Gateway Recompbination
Ol_in3.2 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCTGCTGATGAAGGAATCC | GGGGACCACTTTGTACAAGAAAGCTGGGTCTGAAAGACATTTTAGGGC | Gateway Recompbination
Ip_in3.2 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAGTGACTTACACAGCGAG | GGGGACCACTTTGTACAAGAAAGCTGGGTCTGCAAAATAACATTAAACAC | Gateway Recompbination
qRT-PCR assays:
Gene | Forward | Reverse
angptl4 | CGAGCGCATCAAGCAACA | TCGCTCGTTTTTCATCGTAATCT
egfp | GAAGAAGTCGTGCTGCTTCA | CCTGAAGTTCATCTGCACCA
18S | CACTTGTCCCTCTAAGAAGTTGCA | GGTTGATTCCGATAACGAACGA
tdT | CACCATCGTGGAACAGTACG | GGCCATGTTGTTGTCCTC
rpl32 | CCCTCACCAAACCTAAGATCGT | CTCCAGTTTGCCCTGATCTTG
Dnase I hypersensitivity:
Region | Forward | Reverse
TATA box | CTGAGCAGACTCGCACACTC | CTCATGCTGTTGAAAGGCTAACT
in3.3 | GATGTCATCAGAGGGGTGCT | CTCAGTCCCTCAGAGCTCTACAA
in3.4 | CTGGACGTGTGAGCGTTTTA | GACACAAACACATGTTCAAGCA