Spreadsheet: 87a91c2b-5c79-4022-a5e0-bc1317378519
Sheet: Sheet1

Table S1. Microarray expression analysis derived from CH12F3-2A cells after Spt4 or Spt5 knockdown. The fold change in gene expressions in Spt4 or Spt5 RNAi-treated samples relative to control of three replicates was calculated. The genes that were significantly different using a 2-fold cut-off with a P-value of <0.05 are listed. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7
Up-regulated transcripts by Spt4 knockdown
Name | oligo_sequence | Ensambl_id | Gene_Symbol | RefSeq | description | siSpt4/siControl
 |  |  |  |  |  | ratio | LOG2_ratio
IGHG3 | CAGTTGGTTGCAAGGAGAAATTTTTACCTGCTCCGTGGTGCATGAGGCTCTCCATAACCACCACACACA | ENSMUSG00000076615 | IGHG3 | NM_198640 | Immunoglobulin heavy chain C gene segment [Source:IMGT/GENE-DB;Acc:IGHG3] | 2.712323328035574 | 1.439529168131158
[NAME] | TTGAGCTTGGAGTTCAACCTCAGGGAGTTCCAGGGAATTAAGGCTCCTTCCACATCCCCACTTATAGCCA | ENSMUSG00000047798 | [NAME] | NM_145634 | CD300 antigen like family member F [Source:MarkerSymbol;Acc:MGI:2442359] | 2.675426137038656 | 1.419768699674093
- | GACTTCTTACAACAAGCAGGTCCCAGCAAAGGCAGATATGAGTACTAGAGCCTTCCAAGTCAGCTCCTGA | - | - | - | - | 2.650508915520686 | 1.406269393414251
9130404D14Rik | TGTCCTTGTGAGCCATTTGGGCTTGTCTTTCCCTCTTAGTCACAAGGGTCAAACCAAGAGTCAGTTAGCT | ENSMUSG00000026796 | 9130404D14Rik | NM_146119 | RIKEN cDNA 9130404D14 gene [Source:MarkerSymbol;Acc:MGI:2442910] | 2.610608721237399 | 1.384386242318813
Abca1 | GGTACTTGGTTCTCTTGTTCAGTGTTGCCCATGCCCCTGTCATGGCTTGCCATAGCATCTTTCTGAGAC | ENSMUSG00000015243 | Abca1 | NM_013454 | ATP-binding cassette, sub-family A (ABC1), member 1 [Source:MarkerSymbol;Acc:MGI:99607] | 2.522894830181412 | 1.335080067139357
[NAME] | CATTTCCTTGATTGCGTTCTTTTTACTCATCATCTCCTATGTCTTTCTTCTCACCACTGTCCAGAAGCAG | ENSMUSG00000074955 | [NAME] | NM_146401 | olfactory receptor 1305 [Source:MarkerSymbol;Acc:MGI:3031139] | 2.507769872732287 | 1.326404964287997
Il10ra | CCTGTCTGTATGCAAAGCTTGGAAATTGAAATGTACCTGACGTGCTCCATCCCTAGGAGTGCTGAGTCCC | ENSMUSG00000032089 | Il10ra | NM_008348 | interleukin 10 receptor, alpha [Source:MarkerSymbol;Acc:MGI:96538] | 2.48883125506985 | 1.31546841765254
8030423J24Rik | GGCTGTCTCCTGTTTGATGTGCAGGCCTGGAAACCACCAGGAAAGGCTTCTGTGAGAATGGAGTGAAAT | ENSMUSG00000021600 | 8030423J24Rik | XM_127424 | RIKEN cDNA 8030423J24 gene [Source:MarkerSymbol;Acc:MGI:1924416] | 2.459381319007243 | 1.298295437410956
2210403B10Rik | CCCAGCTAACTCCCAATCATTGGACAGGTCAGTTTTACCTGAGACCTTATTGGGTCATAAGCACAGATGG | ENSMUSG00000034209 | 2210403B10Rik | NM_145216 | RIKEN cDNA 2210403B10 gene [Source:MarkerSymbol;Acc:MGI:1922918] | 2.433905289104316 | 1.283273029350733
- | CAAGGAATTGGCCTTTCGCAAACCTTCCCAAAGCTTGATCCTCCCCAGACCATCACGTCATAGTGTTGCT | - | - | NM_198112 | - | 2.424006951405455 | 1.277393836081407
Olfr981 | AGGGACTATCCACTCACTTTTCCAAACGAGTTTCATATTCAGGCTGCCTTTCTGCGGCCCAAACCGAGTT | ENSMUSG00000046678 | Olfr981 | NM_146286 | olfactory receptor 981 [Source:MarkerSymbol;Acc:MGI:3030815] | 2.3748096079306 | 1.2478118550416
Grn | CCGGCAGGGTTTCAGTGTCACACAGAGAAAGGAACCTGCGAAATGGGTATCCTCCAAGTACCCTGGATG | ENSMUSG00000034708 | Grn | NM_008175 | granulin [Source:MarkerSymbol;Acc:MGI:95832] | 2.330410020918261 | 1.220583810288976
ENSMUSG00000055181 | ATAAAGACAGGCAGCACTCATGACTGATTTCATTCTGCTCTGGGCAATGCCTGCCAACATTGCAAAGGTA | ENSMUSG00000055181 | ENSMUSG00000055181 | - | predicted gene, ENSMUSG00000055181 [Source:MarkerSymbol;Acc:[NAME]] | 2.296918362492297 | 1.199699581086523
Clcf1 | AGAACTATGAGGCGTACAGTCACCTCCTGTGTTACTTGCGTGGCCTCAACCGTCAGGCTGCCACAGCTG | ENSMUSG00000040663 | Clcf1 | NM_019952 | cardiotrophin-like cytokine factor 1 [Source:MarkerSymbol;Acc:MGI:1930088] | 2.275464972805755 | 1.186161378462387
Il28ra | CAGTGGCAACCTTTCAGCCCAGTGGACCTGAGTTCTCTGATGACTTGATCCTTTGTCCCCAGAAGGAACT | ENSMUSG00000062157 | Il28ra | NM_174851 | interleukin 28 receptor alpha [Source:MarkerSymbol;Acc:MGI:2429859] | 2.233643829262026 | 1.159399155953982
Rab13 | CCTCAAGCACTGGCCTGAAAACATCTGACAAGAAGAAGAACAAGTGCTTGTTAGGCTGAGAGCATTTCT | ENSMUSG00000027935 | Rab13 | NM_026677 | RAB13, member RAS oncogene family [Source:MarkerSymbol;Acc:MGI:1927232] | 2.213849409602838 | 1.146557090498817
[NAME] | GAGATCATGACATATCTACACGACTTAGAGATGAAGAACATGGAGGCCCGAGAGAACAATCAGGAGCTCA | ENSMUSG00000073116;ENSMUSG00000070933;ENSMUSG00000048703;ENSMUSG00000067698;ENSMUSG00000073208;ENSMUSG00000051940;ENSMUSG00000073117;ENSMUSG00000033219;ENSMUSG00000069720;ENSMUSG00000073119;ENSMUSG00000067700 | [NAME] | NM_207657 | - | 2.210161315020505 | 1.144151672732869
Gucy2c | TGTGTGTTCCTGACAGTGTGATGAGTGTATACGTCAGAGCCCTGTCCCACAGTTCTCCATGGAGCATCAA | ENSMUSG00000042638 | Gucy2c | XM_132928 | guanylate cyclase 2c [Source:MarkerSymbol;Acc:MGI:106903] | 2.184066873946444 | 1.127017030815386
Cxcr3 | CCTCCCAGATTTCATCTACCTATCAGCCAACTACGATCAGCGCCTCAATGCCACCCATTGCCAGTACAAC | ENSMUSG00000050232 | Cxcr3 | NM_009910 | chemokine (C-X-C motif) receptor 3 [Source:MarkerSymbol;Acc:MGI:1277207] | 2.162317393463415 | 1.112578303076128
Upb1 | ACCAGAAAGAACCACATCCCCAGAGTGGGAGACTTCAACGAGTCCACTTACTACATGGAGGGAAACCTG | ENSMUSG00000033427 | Upb1 | NM_133995 | ureidopropionase, beta [Source:MarkerSymbol;Acc:MGI:2143535] | 2.132608197500249 | 1.09261893827849
- | AGATGTGAAGCTAAGGGACAAGGGAAGGATCTTGTCACTCAGATGCCAACACCTAGGCACATCCACTCTT | - | - | - | - | 2.126941138705062 | 1.088780108487431
Fcgr2b | CTGGGATTGCTGTCGCAGCCATTGTTATTATCCTAGTATCCTTGGTCTATCTCAAGAAAAAGCAGGTTCC | ENSMUSG00000026656 | Fcgr2b | NM_010187 | Fc receptor, IgG, low affinity IIb [Source:MarkerSymbol;Acc:MGI:95499] | 2.119256925964323 | 1.083558501983348
[NAME] | TGTAAGCAGCGAAAGGGTGATCCGTTCTGAAGATGGAGAGACACTGGCCAGGGAATATGGTGTTCCTTT | ENSMUSG00000020732 | [NAME] | NM_021411 | RAB37, member of RAS oncogene family [Source:MarkerSymbol;Acc:MGI:1929945] | 2.113386886473076 | 1.079556897941742
Kti12 | GAAAACCGAGCTTCCTGTTCCCTTACACTCTTACGGGACCTTTTGTTTCTATGGGAAACCGGAACTCTAA | ENSMUSG00000053880 | Kti12 | - | KTI12 homolog, chromatin associated (S. cerevisiae) [Source:MarkerSymbol;Acc:MGI:1923547] | 2.061087550205617 | 1.043405788508802
Vdr | CTACCACCCAAAAGTCAATGTAGACCTACGAAACGGTCAGGTGATGGGCTAGGGACGTATCTTCAAACT | ENSMUSG00000022479 | Vdr | NM_009504 | vitamin D receptor [Source:MarkerSymbol;Acc:MGI:103076] | 2.05412322786199 | 1.038522732236792
- | ACCTGCTAAATAAGTCATCAGAACCCGGACACCAGCGCTGCAACTCTTGGGATGGCAGTCATCTTTCCAG | - | - | NM_146189;NM_178067 | - | 2.050241288357204 | 1.035793707307148
- | GAGACTTAATGGTGGTGTTCAAGTCCCTTCCGTTGTACAGTAGTTATAGGGGAGGTCTAGGTGTTTTTCT | - | - | NM_010658 | - | 2.047610582420393 | 1.03394136756909
Vav3 | TCCAGCAAACCCATCAGTTTAGCTTTCTCTTACTTCATTGGGCCAGACACCATGGATCCAGCCATTAGGA | ENSMUSG00000033721 | Vav3 | NM_020505;NM_146139 | vav 3 oncogene [Source:MarkerSymbol;Acc:MGI:1888518] | 2.046604451776403 | 1.033232298940468
1700023I07Rik | CTCAGCTGAACAGCCCAGCCTGACCTTCAAAAAGCTGAATAAGAAGTCCCAGACCTGGGATGAAGCTAAC | ENSMUSG00000052025 | 1700023I07Rik | XM_284686 | RIKEN cDNA 1700023I07 gene [Source:MarkerSymbol;Acc:MGI:1923955] | 2.041922423304791 | 1.02992805640207
A330070K13Rik | TGTCAGAGAAAACTGCACATCAGAGCCACTCAGAGTAGAGTGCCAACCATTGAAGGACCCCTGGGTTGGA | ENSMUSG00000052014 | A330070K13Rik | NM_198665 | RIKEN cDNA A330070K13 gene [Source:MarkerSymbol;Acc:MGI:2685897] | 2.038883044753503 | 1.027779021262584
Edg1 | GAAATGTGAGAGTTAAGTCGTGCAGACCAAGAGCTGTTTCTTGCTGTCTTTCTCTAACCCCTACAGCACC | ENSMUSG00000045092 | Edg1 | NM_007901 | endothelial differentiation sphingolipid G-protein-coupled receptor 1 [Source:MarkerSymbol;Acc:MGI:1096355] | 2.038349322268613 | 1.027401314662986
Gna15 | GCCTAGTGATGACCCAGGACCCCTATAAAGTGAGCACATTCGAGAAGCCATATGCAGTGGCCATGCAGT | ENSMUSG00000034792 | Gna15 | NM_010304 | guanine nucleotide binding protein, alpha 15 [Source:MarkerSymbol;Acc:MGI:95770] | 2.038167714765219 | 1.027272771476571
Gc | AGATTGATGCAGAAATGATAGACACCCTGCAGTCCTGATCAGGGCCCGTGCACTAGCTTGGATCTTGAA | ENSMUSG00000035540 | Gc | NM_008096 | group specific component [Source:MarkerSymbol;Acc:MGI:95669] | 2.036597924631738 | 1.026161184331066
Bzrap1 | ATTCATTCTGCTGCCCAGAAAACCTCCCGACCTATGGTGGCTGCATTTGACTATAATCCTCGGGAGAACT | ENSMUSG00000034156 | Bzrap1 | NM_172449 | benzodiazapine receptor associated protein 1 [Source:MarkerSymbol;Acc:MGI:2450877] | 2.03478702273529 | 1.024877798263865
Tnxb | CACAGATACCTGATAACCCTATATGGCTTCCATGATGGGCAGCCTGTAGGCTCTGCTCAAATTGAGGCCC | ENSMUSG00000033327 | Tnxb | NM_031176 | tenascin XB [Source:MarkerSymbol;Acc:MGI:1932137] | 2.031844233886824 | 1.022789805843985
Tacr1 | TCATCCTTGGATCTCACCTCCAATGGCTCCTCTCGAAGCAACTCGAAGACCATGACAGAAAGCTCCAGCT | ENSMUSG00000030043 | Tacr1 | NM_009313 | [NAME] 1 [Source:MarkerSymbol;Acc:MGI:98475] | 2.015017621939462 | 1.010792455613544
Cyp4v3 | TCCTTCTATATTTAGTTACACTGTATGATCGCACTTGGTTCAGCCCGAGACATCTAATTAACCCAGCCC | ENSMUSG00000031640 | Cyp4v3 | NM_133969 | [NAME], plasma 1 [Source:MarkerSymbol;Acc:MGI:102849] | 2.013972941206263 | 1.010044300102503
Myo1b | GCACGACTCTCAGCCAAACTAAACAGAAGCTCAACATCGAGATTTCCGATGAGTTCCTGGTACAGTTCA | ENSMUSG00000018417 | Myo1b | NM_010863 | myosin IB [Source:MarkerSymbol;Acc:MGI:107752] | 2.013101889271426 | 1.009420193335559
Casr | ATGGAGGGAGAGGTTACCCGGTTGAGAGTTTTTCTTAGAGTCCCAGGAAAAAGGATGGGTCTTCCCAGG | ENSMUSG00000051980 | Casr | NM_013803 | calcium-sensing receptor [Source:MarkerSymbol;Acc:MGI:1351351] | 2.011621990046457 | 1.008359229436857
Zfp467 | [NAME] | ENSMUSG00000068551 | Zfp467 | NM_020589 | zinc finger protein 467 [Source:MarkerSymbol;Acc:MGI:1916160] | 2.007767819022292 | 1.00559244373472