Spreadsheet: 80dd9903-f176-4efb-84b0-b9e086a91248
Sheet: Sheet1

Table S8: Results of candidate gene overexpression on cellular FC levels | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7
For 29 genes where knockdown with ≥1 siRNA increased or reduced free cholesterol (FC) levels, consequences upon overexpression of a representative cDNA (carboxy-terminally linked to EGFP) on FC were analyzed (see Methods). Filipin staining was performed 24h upon cDNA-transfection and fraction of GFP-positive cells ± SEM was determined. Ratios of FC in GFP-positive relative to non-expressing cells (within the identical dish) and ratios of FC in GFP-positive relative to mock-transfected cells are given as means ± SEM (n=3-4 experiments). Effects were considered as significant (increase, red; decrease, blue) when a two-tailed Student's t-test resulted in p-values <0.01 in 2 (*) or 3-4 (***) experimental replicas.
Gene | cDNA construct |  | Fraction of GFP expressing cells |  | FC-ratio GFPcells/nonGFPcells |  | FC-ratio GFPcells/MOCK
control | GFP-GFP |  | 0.62 ± 0.02 |  | 1.01 ± 0.02 |  | 0.85 ± 0.03
LDLR | LDLR-GFP |  | 0.70 ± 0.04 |  | 5.56 ± 0.43 *** |  | 4.39 ± 0.01 ***
APOA1 | APOA1-GFP |  | 0.49 ± 0.02 |  | 1.00 ± 0.07 |  | 1.01 ± 0.18
APOC1 | APOC1-GFP |  | 0.76 ± 0.07 |  | 1.00 ± 0.04 |  | 0.83 ± 0.01
B4GALT4 | B4GALT4-GFP |  | 0.23 ± 0.04 |  | 1.15 ± 0.07 * |  | 0.89 ± 0.11
BAZ1B | BAZ1B-GFP |  | 0.02 ± 0.00 |  | 2.19 ± 0.32 *** |  | 1.92 ± 0.33 ***
CLPTM1 | CLPTM1-GFP |  | 0.60 ± 0.02 |  | 1.16 ± 0.05 * |  | 1.06 ± 0.07
CXCL12 | CXCL12-GFP |  | 0.28 ± 0.04 |  | 1.12 ± 0.11 |  | 0.71 ± 0.06 ***
FAM174A | FAM174A-GFP |  | 0.66 ± 0.04 |  | 1.20 ± 0.05 * |  | 1.19 ± 0.11 *
FOLH1 | FOLH1-GFP |  | 0.03 ± 0.01 |  | 1.54 ± 0.08 *** |  | 1.33 ± 0.05 ***
HAVCR1 | HAVCR1-GFP |  | 0.25 ± 0.05 |  | 1.88 ± 0.05 *** |  | 1.75 ± 0.06***
LPL | LPL-GFP |  | 0.39 ± 0.04 |  | 1.24 ± 0.07 *** |  | 1.04 ± 0.08
MAU2 | MAU2-GFP |  | 0.51 ± 0.07 |  | 1.28 ± 0.06 *** |  | 1.15 ± 0.03 ***
MLXIPL | MLXIPL-GFP |  | 0.49 ± 0.04 |  | 1.20 ± 0.07 * |  | 1.18 ± 0.20
MYBPHL | MYBPHL-GFP |  | 0.54 ± 0.02 |  | 1.23 ± 0.05 *** |  | 1.12 ± 0.07 ***
NCAN | NCAN-GFP |  | 0.15 ± 0.03 |  | 1.27 ± 0.07 *** |  | 1.11 ± 0.17 *
PAFAH1B1 | PAFAH1B1-GFP |  | 0.73 ± 0.03 |  | 1.18 ± 0.05 |  | 0.73 ± 0.16
POP4 | POP4-GFP |  | 0.45 ± 0.08 |  | 1.23 ± 0.05 * |  | 1.00 ± 0.08
[NAME] | PSRC1-GFP |  | 0.51 ± 0.05 |  | 1.13 ± 0.09 * |  | 0.89 ± 0.04
SEZ6L | SEZ6L-GFP |  | 0.43 ± 0.06 |  | 1.48 ± 0.04 *** |  | 1.17 ± 0.09 *
SIK3 | SIK3-GFP |  | 0.19 ± 0.04 |  | 1.45 ± 0.09 *** |  | 1.05 ± 0.16
SMAD3 | SMAD3-GFP |  | 0.66 ± 0.03 |  | 1.28 ± 0.04 *** |  | 1.10 ± 0.04 *
SORT1 | SORT1-GFP |  | 0.64 ± 0.02 |  | 1.39 ± 0.03 *** |  | 1.25 ± 0.09 ***
TBL2 | TBL2-GFP |  | 0.70 ± 0.05 |  | 1.04 ± 0.09 |  | 0.88 ± 0.09
TM6SF2 | TM6SF2-GFP |  | 0.70 ± 0.05 |  | 1.12 ± 0.02 |  | 0.89 ± 0.03
TMEM57 | TMEM57-GFP |  | 0.07 ± 0.01 |  | 1.36 ± 0.14 * |  | 1.21 ± 0.17 *
TOMM40 | TOMM40-GFP |  | 0.16 ± 0.03 |  | 1.16 ± 0.06 |  | 0.94 ± 0.03
TSSK6 | TSSK6-GFP |  | 0.57 ± 0.03 |  | 1.17 ± 0.06 *** |  | 0.90 ± 0.07
WDR12 | WDR12-GFP |  | 0.13 ± 0.02 |  | 1.36 ± 0.06 *** |  | 1.32 ± 0.10 ***
[NAME] | YJEFN3-GFP |  | 0.60 ± 0.01 |  | 1.39 ± 0.11 *** |  | 1.06 ± 0.08