Spreadsheet: ad0a5515-8d1b-4216-9db6-c4508541ef43
Sheet: Sheet1

Unnamed: 0 | Unnamed: 1 | paste your sequences here (5' - 3') | length | Nb A | Nb C | Nb G | Nb T | Tm NNM (10-40 bp) | Tm LP(n>40 bp) | Choose a Range of size | Unnamed: 11 | Unnamed: 12
 |  |  |  |  |  |  |  |  |  | min | [NAME]
Probe A | â | GCCCCGGGCGGAGAAGGGCGAGGACGAAGAGCGCCGGGCCGCGACA | 46 | 10 | 14 | 22 | 0 | 96.96599886217729 | 85.35781696104061 | 1 | 20 | á
Probe B | â | GCCCCGGGCGGAGAAGGGCGAGGACGAAGAGCGCCGGGCCGCGACT | 46 | 9 | 14 | 22 | 1 | 96.0190376513599 | 85.35781696104061 | 1 | 20 | á
[NAME] | â | AGGAGCCCACCCGCGCCCTCCGACTGCAGACATGGGGAAGAGACGCGGGGACTCCAAAG | 59 | 15 | 20 | 20 | 4 | 95.99238911964159 | 83.46245955499789 | 1 | 20 | á
 |  | Concentration of Monovalent Ions Na+, K+ in [NAME] (default 90 mM) | 90 |  |  |  |  | á
 |  | Concentration of probe in nM (default 0.25 nM) | 0.25 |  |  |  |  | á
 |  | formamide (%) (default 0 mM) | 0 |  |  |  |  | á
Sequences |  | Derived sequences | length | Nb A | Nb C | Nb G | Nb T | Tm NNM (10-40 bp) | Tm LP(n>40 bp)
Probe A | 1 | AAGAGCGCCGGGCCGCGACA | 20 | 5 | 7 | 8 | 0 | 70.18154531721251 | 69.8904256566928
 | 2 | AGAGCGCCGGGCCGCGACA | 19 | 4 | 7 | 8 | 0 | 69.27045913736845 | 70.19305723564017
 | 3 | GAGCGCCGGGCCGCGACA | 18 | 3 | 7 | 8 | 0 | 68.59094714326676 | 70.52931454558168
 | 4 | AGCGCCGGGCCGCGACA | 17 | 3 | 7 | 7 | 0 | 66.92833485497954 | 68.49336683316338
 | 5 | GCGCCGGGCCGCGACA | 16 | 2 | 7 | 7 | 0 | 66.08194209417024 | 68.7654256566928
 | 6 | CGCCGGGCCGCGACA | 15 | 2 | 7 | 6 | 0 | 62.18206537475714 | 66.34042565669279
 | 7 | GCCGGGCCGCGACA | 14 | 2 | 6 | 6 | 0 | 56.62585020234916 | 63.56899708526422
 | 8 | CCGGGCCGCGACA | 13 | 2 | 6 | 5 | 0 | 51.39880691331648 | 60.37119488746202
 | 9 | CGGGCCGCGACA | 12 | 2 | 5 | 5 | 0 | 45.51456389180128 | 56.64042565669279
 | 10 | GGGCCGCGACA | 11 | 2 | 4 | 5 | 0 | 36.87048382182416 | 52.23133474760189
Probe B | 1 | AAGAGCGCCGGGCCGCGACT | 20 | 4 | 7 | 8 | 1 | 68.54351533857573 | 69.8904256566928
 | 2 | AGAGCGCCGGGCCGCGACT | 19 | 3 | 7 | 8 | 1 | 67.56749581733419 | 70.19305723564017
 | 3 | GAGCGCCGGGCCGCGACT | 18 | 2 | 7 | 8 | 1 | 66.82455122788349 | 70.52931454558168
 | 4 | AGCGCCGGGCCGCGACT | 17 | 2 | 7 | 7 | 1 | 65.13866710065383 | 68.49336683316338
 | 5 | GCGCCGGGCCGCGACT | 16 | 1 | 7 | 7 | 1 | 64.2227873295555 | 68.7654256566928
 | 6 | CGCCGGGCCGCGACT | 15 | 1 | 7 | 6 | 1 | 60.2772927981673 | 66.34042565669279
 | 7 | GCCGGGCCGCGACT | 14 | 1 | 6 | 6 | 1 | 54.69754801278992 | 63.56899708526422
 | 8 | CCGGGCCGCGACT | 13 | 1 | 6 | 5 | 1 | 49.44205812244939 | 60.37119488746202
 | 9 | CGGGCCGCGACT | 12 | 1 | 5 | 5 | 1 | 43.54104021880124 | 56.64042565669279
 | 10 | GGGCCGCGACT | 11 | 1 | 4 | 5 | 1 | 34.94113547386353 | 52.23133474760189
[NAME] | 1 | AGGAGCCCACCCGCGCCCTC | 20 | 3 | 11 | 5 | 1 | 67.9160171202318 | 71.9404256566928
 | 2 | AGGAGCCCACCCGCGCCCT | 19 | 3 | 10 | 5 | 1 | 66.36713453177146 | 70.19305723564017
 | 3 | AGGAGCCCACCCGCGCCC | 18 | 3 | 10 | 5 | 0 | 65.56625189502654 | 70.52931454558168
 | 4 | AGGAGCCCACCCGCGCC | 17 | 3 | 9 | 5 | 0 | 62.12461645306518 | 68.49336683316338
 | 5 | AGGAGCCCACCCGCGC | 16 | 3 | 8 | 5 | 0 | 58.2444156695937 | 66.2029256566928
 | 6 | AGGAGCCCACCCGCG | 15 | 3 | 7 | 5 | 0 | 53.66833755240076 | 63.60709232335947
 | 7 | AGGAGCCCACCCGC | 14 | 3 | 7 | 4 | 0 | 47.24425408993419 | 60.64042565669281
 | 8 | AGGAGCCCACCCG | 13 | 3 | 6 | 4 | 0 | 41.05318115640755 | 57.21734873361587
 | 9 | [NAME] | 12 | 3 | 6 | 3 | 0 | 32.35731453680142 | 53.22375899002613
 | 10 | AGGAGCCCACC | 11 | 3 | 5 | 3 | 0 | 23.80159163620998 | 48.50406202032916