Spreadsheet: 1d78f211-01b0-4b37-ac9e-73610d57d455
Sheet: Sheet1

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 | iTRAQ/MudPit Mass Spec Sample Submission Form
 | Instructions: Please enter all requested information below and then click the Finalize Worksheet button before uploading the worksheet to the Proteus LIMS system.   NOTE: If using Microsoft Excel 2007, please save the file in Excel 97-2003 format.  If using a MAC, you may need to use Excel 2004 or 2011 in order to run the VBA macros in this document.
 | Section 1: Please fill in all of your lab's information.
 | Researcher Name:
 | Phone #:
 | [NAME]:
 | Principal Investigator:
 | Department
 | Experiment Name:
 | Lab Address and [NAME] if Outside of HMC: | We are an HMC lab.
 | Section 2: Please fill in all sample names as well as any special conditions for samples that have the named iTRAQ label.
 | iTRAQ Label | Sample Names |  |  | Special Conditions
 | 113
 | 114
 | 115
 | 116
 | 117
 | 118
 | 119
 | 121
 | iTRAQ Reagents Lot #
 | Section 3: Please fill in all sample information.
 | Samples Were Digested With…? | Samples Already Alkylated? | Total Protein (ug) in Submitted Pool Sample (Number Only) | Volume (uL) (Number Only) | Species | Tissue Source | [NAME]
 | Sample/Name | UDT/DNA Sequencing/Concentration | UDT/DNA Sequencing/Primer Name | UDT/DNA Sequencing/Primer Concentration | UDT/DNA Sequencing/Template Size |  | UDT/DNA Sequencing/Notes
 |  | Section 4: For 4PLEX [NAME] ONLY, fill in iTRAQ Correction Values below for all labels used (from sheet that came with iTRAQ label kit).
 |  | iTRAQ Correction Table | % of -2 | % of -1 | % of 0 | % of +1 | % of +2
 |  | iTRAQ114 | 0 | 1 | 92.9 | 5.9 | 0.2
 |  | iTRAQ115 | 0 | 2 | 192.3 | 5.6 | 0.1
 |  | iTRAQ116 | 0 | 3 | 92.4 | 4.5 | 0.1
 |  | iTRAQ117 | 0 | 4 | 92.3 | 3.5 | 0.1
 |  |  |  |  |  |  | <TABLE HEADER> | Sample/Name | UDT/iTRAQ_MudPit/Alkylation | UDT/iTRAQ_MudPit/Total Protein Amount (ug) | Sample/Volume | UDF/Species | UDF/Tissue Source | UDF/Subcellular Fraction | UDT/iTRAQ_MudPit/Not Digested | UDT/iTRAQ_MudPit/Trypsin Digested | UDT/iTRAQ_MudPit/Glu-C Digested | UDT/iTRAQ_MudPit/Lys-C Digested | UDT/iTRAQ_MudPit/CNBr Digested | UDT/iTRAQ_MudPit/Other Digestion Method | UDT/iTRAQ_MudPit/113 Label Sample Name | UDT/iTRAQ_MudPit/114 Label Sample Name | UDT/iTRAQ_MudPit/115 Label Sample Name | UDT/iTRAQ_MudPit/116 Label Sample Name | UDT/iTRAQ_MudPit/117 Label Sample Name | UDT/iTRAQ_MudPit/118 Label Sample Name | UDT/iTRAQ_MudPit/119 Label Sample Name | UDT/iTRAQ_MudPit/121 Label Sample Name | UDT/iTRAQ_MudPit/iTRAQ_Reagent_LotNumber | UDT/iTRAQ_MudPit/114-2 | UDT/iTRAQ_MudPit/114-1 | UDT/iTRAQ_MudPit/114-0 | UDT/iTRAQ_MudPit/114+1 | UDT/iTRAQ_MudPit/114+2 | UDT/iTRAQ_MudPit/115-2 | UDT/iTRAQ_MudPit/115-1 | UDT/iTRAQ_MudPit/115-0 | UDT/iTRAQ_MudPit/115+1 | UDT/iTRAQ_MudPit/115+2 | UDT/iTRAQ_MudPit/116-2 | UDT/iTRAQ_MudPit/116-1 | UDT/iTRAQ_MudPit/116-0 | UDT/iTRAQ_MudPit/116+1 | UDT/iTRAQ_MudPit/116+2 | UDT/iTRAQ_MudPit/117-2 | UDT/iTRAQ_MudPit/117-1 | UDT/iTRAQ_MudPit/117-0 | UDT/iTRAQ_MudPit/117+1 | UDT/iTRAQ_MudPit/117+2 | UDT/iTRAQ_MudPit/ExternalAddress | UDT/iTRAQ_MudPit/SpecialConditions | Container/Name | Container/Type | Sample/Well Location
 |  |  |  |  |  |  | </TABLE HEADER> |  |  |  |  |  |  |  | False | False | False | False | False | False | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | 0 | 1 | 92.9 | 5.9 | 0.2 | 0 | 2 | 192.3 | 5.6 | 0.1 | 0 | 3 | 92.4 | 4.5 | 0.1 | 0 | 4 | 92.3 | 3.5 | 0.1 | We are an HMC lab. | [NAME] | iTRAQ201495143749 | Tube | 1:1
 |  |  |  |  |  |  | <SAMPLE ENTRIES> |  |  |  |  |  |  |  | False | False | False | False | False | False | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | [NAME] | 0 | 1 | 92.9 | 5.9 | 0.2 | 0 | 2 | 192.3 | 5.6 | 0.1 | 0 | 3 | 92.4 | 4.5 | 0.1 | 0 | 4 | 92.3 | 3.5 | 0.1 | We are an HMC lab. | [NAME] | iTRAQ201495143749 | Tube | 1:1
 |  |  |  |  |  |  | </SAMPLE ENTRIES> |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | 2014-09-05 14:37:49.420000
 | False | No Alkylation |  |  | Human | Serum | [NAME]
 | False | Iodoacetamide |  |  | Rat | Plasma | [NAME]
 | False | Iodoacetic Acid |  |  | [NAME] | Red Blood Cells | Nuclear Prep
 | False | MMTS |  |  | Yeast | White Blood Cells | Membrane Prep
 | False | Vinylpyridine |  |  | Bovine | [NAME] | Mitochondrial Prep
 | False | Other (type in directly) |  |  | Pig | CSF | Microsomal Prep
 |  |  |  |  | Monkey | Cell Culture | [NAME]
 |  |  |  |  | E. coli | Brain | Supernatant fraction
 |  |  |  |  | Compound (chemical) | Colon | Immunoprecipitation
 |  |  |  |  | Arabidopsis | Heart | Molecule or chemical
 |  |  |  |  | C dificile | Kidney
 |  |  |  |  | Other | Liver
 |  |  |  |  |  | Muscle
 |  |  |  |  |  | Pancreas
 |  |  |  |  |  | [NAME]
 |  |  |  |  |  | Skin
 |  |  |  |  |  | [NAME]
 |  |  |  |  |  | Stomach
 |  |  |  |  |  | Guard Cells
 |  |  |  |  |  | Other Tissue
 |  |  |  |  |  | Chemical Compound