Spreadsheet: 567274b5-a8f4-46f4-b9d5-d426215b8768
Sheet: Sheet1

 | Polymerase |  |  | [NAME] pol |  | [NAME] pol |  |  | [NAME] pol |  | [NAME] pol
 | Genotype notes |  |  | URA3 OR1 |  | msh2Δ URA3 OR1 |  |  | [NAME] |  | msh2Δ ATT0 URA3 OR1
 | Mutation rate (x10^-7) |  |  | 0.18 |  | 7.2 |  |  | 0.67 |  | 7.6
 | 95% Confidence range |  |  | (0.14-0.23) |  | (3.5-15) |  |  | (0.50-0.90) |  | (5.9-9.8)
 | Total Mutants Sequenced |  |  | 211 |  | 195 |  |  | 156 |  | 92
 | # w/ WT yURA3 |  |  | 70 |  | 15 |  |  | 32 |  | 14
 | # w/ single mutation |  |  | 141 |  | 180 |  |  | 122 |  | 78
 | # w/ multiple mutations |  |  | 0 |  | 0 |  |  | 2 |  | 0
 | Total URA3 mutations |  |  | 141 |  | 180 |  |  | 126 |  | 78
 |  |  |  | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor
 | A to T |  |  | 9 | 0.0077 | 1 | 0.037 | 4.8 | 7 | 0.030 | 0 | ≤ 0.083 | [NAME] 2.7
 | T to A |  |  | 7 | 0.0060 | 1 | 0.037 | 6.2 | 1 | 0.0043 | 0 | ≤ 0.083 | [NAME] 19
 | position | original sequence | mutation(s) | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor
 | 279 | A | T | 4 | 0.0034 | 0 | ≤ 0.037 | [NAME] 11 | 2 | 0.0086 | 0 | ≤ 0.083 | [NAME] 9.6
 | 686 | A | T | 2 | 0.0017 | 0 | ≤ 0.037 | [NAME] 22 | 1 | 0.0043 | 0 | ≤ 0.083 | [NAME] 19
 | Polymerase |  |  | pol2M644G |  | pol2M644G |  |  | pol2M644G |  | pol2M644G
 | Genotype notes |  |  | URA3 average |  | msh2Δ URA3 ave. |  |  | [NAME] |  | msh2Δ ATT0 URA3 OR1
 | Mutation rate (x10^-7) |  |  | 1.2 |  | 180 |  |  | 1.3 |  | 170
 | 95% Confidence range |  |  | (0.71-2.3) |  | (100-270) |  |  | (0.87-1.8) |  | (100-280)
 | Total Mutants Sequenced |  |  | 588 |  | 587 |  |  | 212 |  | 361
 | # w/ WT yURA3 |  |  | 228 |  | 73 |  |  | 94 |  | 37
 | # w/ single mutation |  |  | 359 |  | 498 |  |  | 116 |  | 324
 | # w/ multiple mutations |  |  | 1 |  | 16 |  |  | 2 |  | 0
 | Total URA3 mutations |  |  | 361 |  | 530 |  |  | 120 |  | 324
 |  |  |  | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor
 | Substitutions |  |  | 317 | 0.60 | 281 | 87 | 140 | 90 | 0.55 | 168 | 79 | 140
 | Transitions |  |  | 60 | 0.12 | 175 | 54 | 450 | 27 | 0.17 | 116 | 55 | 330
 | G-dT |  |  | 21 | 0.037 | 65 | 16 | 440 | 8 | 0.049 | 11 | 5.2 | 110
 | Transversions |  |  | 257 | 0.45 | 106 | 33 | 73 | 63 | 0.39 | 52 | 24 | 63
 | T-dT |  |  | 174 | 0.17 | 10 | 2.8 | 17 | 30 | 0.18 | 14 | 6.6 | 36
 | C-dT |  |  | 20 | 0.039 | 36 | 8.9 | 230 | 11 | 0.067 | 20 | 9.4 | 140
 | Frameshifts |  |  | 26 | 0.053 | 247 | 73 | 1,400 | 24 | 0.15 | 154 | 73 | 490
 | -1 |  |  | 20 | 0.041 | 214 | 62 | 1,500 | 23 | 0.14 | 147 | 69 | 490
 | ΔT |  |  | 7 | 0.010 | 114 | 33 | 3,300 | 11 | 0.067 | 72 | 34 | 500
 | +1 |  |  | 6 | 0.0092 | 33 | 9.9 | 1,100 | 1 | 0.0061 | 7 | 3.3 | 540
 | Multi-base Errors |  |  | 18 | 0.035 | 2 | 0.62 | 18 | 6 | 0.037 | 2 | 0.94 | 26
 | Total |  |  | 361 | 0.71 | 530 | 160 | 230 | 120 | 0.74 | 324 | 150 | 210
 | position | original sequence | mutation(s) | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor
 | OR1 174 | A | Δ | 0 | ≤ 0.0050 | 55 | 30 | ≤ 6,000 | 4 | 0.025 | 57 | 27 | 1,100
 | OR2 201 | T | Δ | 0 | ≤ 0.0034 | 11 | 7.8 | ≤ 2,300
 | OR2 255 | T | Δ | 1 | 0.0034 | 29 | 21 | 6,100
 | OR1 279 | A | T | 24 | 0.12 | 1 | 0.54 | 4.5 | 18 | 0.11 | 2 | 0.94 | 8.5
 | OR1 310 | C | T | 1 | 0.0050 | 3 | 1.6 | 330 | 2 | 0.012 | 1 | 0.47 | 38
 | OR1 345 | G | T | 2 | 0.0099 | 6 | 3.2 | 330 | 3 | 0.018 | 2 | 0.94 | 51
 | OR2 608 | G | A | 1 | 0.0034 | 8 | 5.7 | 1,700
 | OR1 679 | G | T | 0 | ≤ 0.0050 | 14 | 7.6 | ≤ 1,500 | 1 | 0.0061 | 8 | 3.8 | 610
 | OR1 686 | A | T | 136 | 0.68 | 0 | ≤ 0.54 | [NAME] 0.78 | 9 | 0.055 | 4 | 1.9 | 35
 | OR2 764 | G | A | 1 | 0.0034 | 15 | 11 | 3,200
 | Polymerase |  |  | pol1L868M |  | pol1L868M |  |  | pol3L612M |  | pol3L612M
 | Genotype notes |  |  | URA3 average |  | msh2Δ URA3 ave. |  |  | URA3 average |  | msh2Δ URA3 ave.
 | Mutation rate (x10^-7) |  |  | 1.4 |  | 530 |  |  | 1.8 |  | 220
 | 95% Confidence range |  |  | (1.2-7.6) |  | (350-860) |  |  | (1.1-2.7) |  | (170-280)
 | Total Mutants Sequenced |  |  | 544 |  | 350 |  |  | 488 |  | 342
 | # w/ WT yURA3 |  |  | 256 |  | 6 |  |  | 117 |  | 4
 | # w/ single mutation |  |  | 288 |  | 344 |  |  | 371 |  | 338
 | # w/ multiple mutations |  |  | 0 |  | 0 |  |  | 0 |  | 0
 | Total URA3 mutations |  |  | 288 |  | 344 |  |  | 371 |  | 338
 |  |  |  | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor
 | Substitutions |  |  | 248 | 0.63 | 308 | 470 | 740 | 319 | 1.2 | 163 | 110 | 94
 | Transitions |  |  | 99 | 0.24 | 182 | 250 | 1,000 | 208 | 0.76 | 141 | 90 | 120
 | G-dT |  |  | 52 | 0.10 | 135 | 150 | 1,500 | 123 | 0.45 | 102 | 67 | 150
 | Transversions |  |  | 149 | 0.34 | 126 | 150 | 440 | 111 | 0.37 | 22 | 11 | 30
 | C-dT |  |  | 80 | 0.15 | 109 | 120 | 770 | 51 | 0.15 | 17 | 5.2 | 35
 | Frameshifts |  |  | 26 | 0.066 | 35 | 53 | 800 | 54 | 0.19 | 173 | 110 | 610
 | -1 |  |  | 22 | 0.054 | 30 | 44 | 820 | 48 | 0.17 | 162 | 100 | 630
 | ΔT |  |  | 6 | 0.015 | 9 | 13 | 860 | 15 | 0.043 | 98 | 64 | 1,500
 | +1 |  |  | 4 | 0.0089 | 5 | 5.9 | 660 | 6 | 0.020 | 11 | 7.2 | 350
 | Multi-base Errors |  |  | 14 | 0.036 | 1 | 2.8 | 78 | 9 | 0.021 | 2 | 2.6 | 120
 | Total |  |  | 288 | 0.73 | 344 | 520 | 710 | 382 | 1.4 | 338 | 220 | 160
 | position | original sequence | mutation(s) | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor | mutation count | rate (x10-7) | mutation count | rate (x10-7) | correction factor
 | OR2 174 | A | Δ | 1 | 0.0055 | 2 | 5.7 | 1,000 | 1 | 0.0095 | 20 | 26 | 2,800
 | OR1 201 | T | Δ | 0 | ≤ 0.0047 | 2 | 6.4 | ≤ 1,400 | 1 | 0.0057 | 25 | 33 | 5,800
 | OR1 255 | T | Δ | 1 | 0.0047 | 1 | 3.2 | 680 | 0 | ≤ 0.0057 | 28 | 37 | ≤ 6,500
 | OR2 310 | C | T | 1 | 0.0055 | 3 | 8.5 | 1,500 | 45 | 0.43 | 32 | 42 | 98
 | OR2 345 | G | T | 22 | 0.12 | 11 | 31 | 260 | 24 | 0.23 | 7 | 9.2 | 40
 | OR1 608 | G | A | 7 | 0.033 | 6 | 19 | 580 | 7 | 0.040 | 4 | 5.3 | 130
 | OR2 679 | G | T | 11 | 0.061 | 41 | 120 | 1,900 | 4 | 0.038 | 3 | 3.9 | 100
 | OR1 764 | G | A | 12 | 0.057 | 51 | 160 | 2,900 | 17 | 0.097 | 16 | 21 | 220