Spreadsheet: 3a4c287d-4cc3-4124-9292-7ee2a9f5ef02
Sheet: Sheet1

Supplementary table 1: Categories of genes highly expressed in the ST and SR
 |  | Genes highly expressed in ST |  |  |  | Genes highly expressed in SR
 |  | STV | ST3 | ST6 |  | SRV | SR3 | SR6 | Upregulated Compared to whole body(Flyatlas)
[NAME]
 | CG1299 | 2501 | 3861 | 4042 |  | 334 | 60 | 144 | Head, crop, [NAME] (a,l),
 | CG9897 | 1 | 14 | 11 |  | 1487 | 807 | 5681 | ST(m,v)
 | CG10469 | 89 | 134 | 93 |  | 98 | 398 | 214 | H.gut, [NAME] (a,l), ST(m)
 | CG13318 | 508 | 590 | 459 |  | 42 | 9 | 16 | Head, ST (m,v)
 | CG17012 | 4 | 15 | 23 |  | 413 | 36 | 157 | ST (m,v)
 | CG17234 | 73 | 2 | 3 |  | 737 | 2 | 1180 | ST (m,v)
 | CG17239 | 12 | 1 | 1 |  | 552 | 27 | 1858 | ST (m,v)
 | CG18125 | 137 | 5 | 6 |  | 1206 | 59 | 2754 | ST (m,v)
 | CG30371 | 26 | 10 | 16 |  | 1182 | 118 | 2563 | Crop, M.tubule (a,l), SG (a,l), ST (m,v)
 | CG31681 | 6 | 7 | 5 |  | 380 | 40 | 1576 | ST (m,v)
 | CG32277 | 27 | 35 | 34 |  | 2063 | 691 | 5569 | ST (m,v)
 | CG32834 | 20 | 26 | 12 |  | 2296 | 1199 | 6506 | ST (m,v)
Female gamete generation/ lipid particle
 | Yp1 | 7 | 11 | 7 |  | 81 | 25 | 41 | None
 | Yp2 | 28 | 41 | 14 |  | 253 | 61 | 115 | None
 | Yp3 | 34 | 67 | 24 |  | 219 | 107 | 173 | Head
Ion transport
 | blw | 113 | 148 | 139 |  | 60 | 66 | 45 | Head, Crop, M.gut(a,l), H.gut(a,l), M.tubule, TAG, SG(a,l), ST (m,v)
 | CG10413 | 717 | 723 | 798 |  | 159 | 23 | 29 | Crop, M.gut (a,l), H.gut(a,l), M.tubule(a,l), Ovary, Acc, TAG, SG, ST(m,v)
 | CG4462 | 2523 | 2615 | 3470 |  | 193 | 16 | 38 | Brain, Head, M.gut(a,l), H.gut(a,l), M.tubule, TAG, SG(a,l),
 | CG5687 | 1516 | 2352 | 3002 |  | 45 | 768 | 160 | Brain, Head, TAG
 | CG8029 | 255 | 323 | 298 |  | 69 | 96 | 69 | Brain, M.gut(a,l), H.gut(a,l), M.tubule(a,l), SG (a,l),
 | Esp | 974 | 1312 | 1344 |  | 78 | 90 | 39 | H.gut(a,l), ST(m,v)
 | l(2)01810 | 546 | 735 | 904 |  | 59 | 56 | 44 | M.gut (a,l), [NAME] (a,l), M.tubule, SG,
 | [NAME] | 81 | 112 | 106 |  | 54 | 121 | 102 | Brain, Head, Crop, M.gut(a,l), H.gut(a,l), M.tubule(a,l), [NAME], TAG, SG(a,l), ST(m,v)
 | sesB | 115 | 128 | 133 |  | 40 | 89 | 60 | H.gut(a,l), M.tubule, TAG, SG,  M.gut(l), ST(m,v)
 | vha55 | 233 | 325 | 412 |  | 118 | 126 | 74 | Brain, [NAME], M.gut, H.gut(a,l), M.tubule (a,l), Ovary, TAG, SG(a,l).
 | vha68-2 | 170 | 216 | 216 |  | 22 | 33 | 19 | Brain, [NAME], M.gut, H.gut(a,l), M.tubule (a,l), Ovary, TAG, SG(a,l).
 | VhaPPA1-1 | 265 | 345 | 299 |  | 66 | 168 | 107 | Brain, M.gut (a,l), [NAME] (a,l), M. tubule (a,l), TAG, SG (a,l)
Transmembrane receptor
 | CG8444 | 396 | 506 | 418 |  | 86 | 361 | 327 | Brain, Head, Crop, M.gut(a,l), H.gut(a,l), M.tubule (a,l), Fatbody, TAG, SG (a,l)
 | dscam | 57 | 68 | 62 |  | 303 | 2099 | 1856 | Crop, M.gut (a,l), M. tubule, Acc, Fatbody, SG(a,l), ST(m)
Carbohydrate metabolism
 | CG7998 | 110 | 155 | 148 |  | 65 | 163 | 124 | Brain, Crop, M.gut(a,l) , H.gut(a,l), [NAME]), SG(a), ST(m,v)
 | tps1 | 68 | 127 | 76 |  | 486 | 296 | 690 | Brain, M. tubule, ST(m,v)
 | treh | 175 | 266 | 314 |  | 26 | 169 | 49 | Brain, [NAME], M.gut, [NAME], Testis, [NAME], TAG, SG(a,l),
Lipid metabolism/transport
 | CG3523 | 76 | 107 | 82 |  | 262 | 54 | 97 | Head, Crop, M.gut (l), ST (v,m)
 | RfaBp | 97 | 104 | 51 |  | 343 | 95 | 104 | Head, Fatbody, ST(m,v)
 | Sap-r | 85 | 84 | 73 |  | 85 | 65 | 55 | Head, Crop, M.gut(a,l), H.gut(a,l), M.tubule(a,l), [NAME],  SG(a,l), ST(m,v)
Oxoreductase
 | Adh | 22 | 42 | 26 |  | 39 | 34 | 33 | Head, Crop, M.tubule, Fatbody, SG (a)
 | Aldh | 66 | 118 | 68 |  | 91 | 136 | 98 | Head, Crop, M.tubule, SG(a), ST (m,v)
 | ERp60 | 77 | 48 | 47 |  | 21 | 7 | 7 | M.tubule(a,l), Acc, Fatbody, SG(a,l), M.gut(l), [NAME]), ST(m,v)
 | Gapdh2 | 141 | 301 | 186 |  | 104 | 128 | 88 | Head, Crop, M.gut (l), [NAME] (a,l), M.tubule, Ovary, TAG, SG (a), ST(m,v)
 | Gpdh | 202 | 327 | 226 |  | 218 | 94 | 137 | Head, M.tubule(a), SG(a), ST(m,v)
Immunity/Defense
 | Def | 1216 | 1100 | 655 |  | 18 | 17 | 15 | ST (m,v)
 | Drs | 29 | 31 | 31 |  | 29 | 114 | 57 | Fatbody, SG(a), ST(m,v)
 | Rel | 767 | 630 | 796 |  | 741 | 76 | 175 | Head, Crop, M.gut(a,l), H.gut(a,l), M. tubule(a,l), Acc, Fatbody, SG(a), ST(m,v)
 | TepIV | 212 | 190 | 174 |  | 55 | 58 | 27 | Head, Crop, M.gut, [NAME] (a,l), [NAME] (l), Fatbody, ST(m,v)
Chemosensory
 | pst | 140 | 133 | 112 |  | 178 | 85 | 89 | Head, Crop, M.gut(a,l), H.gut(a,l), M.tubule, Acc, Fatbody, SG(a,l), ST(m,v)
 | visgun | 283 | 400 | 342 |  | 100 | 99 | 80 | Brain, Head, Crop, H.gut(a,l), M.tubule(a,l), Acc, Fatbody, TAG, SG(a,l), ST(m,v)
Metal ion binding
 | Aph-4 | 448 | 1102 | 1625 |  | 97 | 1152 | 274 | M.tubule(a,l), [NAME]
 | FER1hch | 78 | 126 | 104 |  | 64 | 44 | 48 | Head, M.gut(a,l), H.gut(a,l), M.tubule(a,l), Fatbody, TAG, ST(m,v)
 | FER2lch | 119 | 165 | 166 |  | 73 | 67 | 56 | Head, M.gut(a,l), [NAME], M.tubule(a,l), TAG, SG(a,l), ST(m,v)
 | MtnA | 102 | 154 | 137 |  | 7 | 153 | 76 | M.gut, [NAME], M.tubule(a,l), SG,
 | Vha13 | 263 | 352 | 402 |  | 94 | 143 | 101 | M.gut(a,l), H.gut(a,l), M.tubule(a,l), TAG, SG(a,l)
 | [NAME] | 139 | 139 | 160 |  | 17 | 20 | 12 | M.gut(a,l), H.gut(a,l), M.tubule(a,l), SG(a,l),
 | Vha26 | 136 | 187 | 178 |  | 32 | 41 | 18 | Brain, M.gut(a,l), H.gut(a,l), M.tubule(a,l), TAG, SG(a,l),
Muscle contraction/development
 | Mp20 | 687 | 1186 | 1490 |  | 44 | 102 | 58 | Crop, M.gut(a,l), H.gut(a,l),
 | TM1 | 363 | 782 | 1205 |  | 20 | 238 | 100 | Acc, SG(l)
 | Tm2 | 559 | 1192 | 1596 |  | 50 | 141 | 64 | H.gut(a,l),
 | wupa | 142 | 267 | 430 |  | 2 | 4 | 2 | Crop, H.gut(a,l), [NAME]
 | Mhc | 245 | 459 | 582 |  | 38 | 72 | 35 | Head, Crop, H,gut(a,l)
 | Zeelin1 | 335 | 640 | 1012 |  | 62 | 64 | 40 | Crop, H.gut(a,l)
Cell morphogenesis
 | mfas | 1916 | 3014 | 3032 |  | 186 | 37 | 96 | Brain, head, crop, H.gut(a,l), testis, [NAME], TAG
 | moe | 406 | 241 | 355 |  | 115 | 83 | 59 | Head, crop, M.gut(a,l), H.gut(a,l), M.tubule(a,l), Ovary, Acc, TAG, SG(a,l), ST(m,v)
Receptor binding
 | Idgf4 | 190 | 238 | 206 |  | 12 | 45 | 34 | Head, H.gut(a,l), Fatbody, TAG, ST(m,v)
 | Idgf1 | 582 | 432 | 362 |  | 356 | 78 | 262 | Head, Crop, H.gut(a,l), Fatbody, ST(m,v)
Other
Signal transduction | CG18067 | 25 | 3 | 2 |  | 16 | 50 | 31 | Head, H.gut, Acc, Fatbody, TAG, SG, ST(m,v)
Unknown (Serine protease) | CG18628 | 997 | 2706 | 1414 |  | 1 | 1 | 1 | Testis
sugar binding | CG3244 | 1096 | 1137 | 1056 |  | 170 | 71 | 92 | Head, Crop, H.gut(a,l),ST(m)
Carbon-oxygen lyase | CG5379 | 355 | 83 | 458 |  | 6845 | 2495 | 11985 | Brain, M.tubule, TAG, ST(m,v)
Calcium ion binding | CG6426 | 2 | 8 | 4 |  | 748 | 271 | 1130 | Head, Crop, H.gut(a,l), ST(m,v)
Water transport | CG7777 | 166 | 202 | 153 |  | 77 | 179 | 86 | Ovary, M.tubule (l), TAG, SG(a), [NAME]), ST(m,v)
neurotransmitter | Chc | 192 | 156 | 212 |  | 256 | 97 | 82 | Brain, Head, Crop, M.gut(a,l), H.gut(a,l), M.tubule(a,l), Ovary, Acc, Fatbody, TAG, SG(a,l), ST(m,v)
Cysteine proteinase | Cp1 | 83 | 151 | 127 |  | 51 | 122 | 105 | Head, M.gut, [NAME]), [NAME], M.tubule(l), ST(m,v)
protease inhibitor | [NAME] | 257 | 282 | 310 |  | 217 | 84 | 135 | Head, Crop, H.gut(a,l), M.tubule, Acc, Fatbody, SG(a,l), ST(m,v)
Chaperone binding | Hsc70-4 | 74 | 46 | 80 |  | 19 | 21 | 28 | Crop, H.gut(a,l), M.gut(a,l), M.tubule(a,l),Acc, SG(a,l), ST(m.v)
JH metabolism | jhamt | 6320 | 2401 | 2095 |  | 1555 | 74 | 81 | Brain, Head
Intracellular signaling cascade | regucalcin | 614 | 1591 | 1316 |  | 88 | 120 | 119 | Brain, Head, M.tubule(a,l), testis, Acc, Fatbody, SG(l),
metalloprotease | sda | 1826 | 1111 | 1286 |  | 227 | 52 | 61 | Brain, Head, M.gut(a,l), H.gut(a,l), M.tubule, [NAME], TAG, SG(a,l), ST(m,v)
[NAME] | Sec61beta | 138 | 79 | 70 |  | 318 | 227 | 232 | M.gut(a,l), [NAME]), M.tubule, Acc, Fatbody, SG(a,l), ST(m,v)
Serine protease inhibitor | Spn5 | 155 | 217 | 213 |  | 11 | 15 | 9 | Head, [NAME]), ST(m,v)
[NAME] binding | twinstar | 144 | 110 | 123 |  | 74 | 57 | 50 | Crop, M.gut(a,l), H.gut(a,l), M.tubule(l), Sg(l)
Ranked gene expression of genes in the spermathecae and seminal receptacle. For example, CG1299 is the 2501th most expressed in the virgin spermathecae (STV); it is the 334th most expressed gene in virgin seminal receptacle (SRV). Shading indicates that the gene was among the top 100 expressed genes. Brain; Head(including brain); Crop; M.gut = Midgut; [NAME]; M.tubule =  Malpighian tubule;  Ovary; Testis; Male Accessory Glands = Acc; Fatbody;  ST = Spermatheca; Thoracico-abdominal Ganglia = TAG, [NAME] = SG; l = larval, a = adult; m = mated; v= virgin; SR = seminal receptacle. V= virgin; 3 = 3 hours post-mating; 6 = six hours post-mating.