Spreadsheet: ae7f5c0e-e550-469e-97e0-72ced7bfce93
Sheet: Sheet1

Table. S3. Proteins significantly changed in the culture supernatants of (WT + gramicidin) compared to (WT - gramicidin). | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7
 |  | WT - gramicidin |  | WT + gramicidin
Protein1 | Description2 | Mean3 | SD4 | Mean3 | SD4 | p value5 | WT+gramicidin/WT-gramicidin6
gi|150373067 | |dbj|BAF66327.1| immunoglobulin G binding protein A precursor (protein A) | 3.7289126057529 | 2.523991169930421 | 14.87365166238561 | 3.359169433010751 | 0.01007546784150598 | 3.988736995186964
gi|150373071 | |dbj|BAF66331.1| siderophore compound ABC transporter binding protein | 2.739763756624713 | 0.6117993334448049 | 9.233152921785829 | 3.820778819976826 | 0.04382785527973661 | 3.370054406866354
gi|150373089 | |dbj|BAF66349.1| superoxide dismutase | 2.050190282642256 | 1.007516450815842 | 9.217158933868884 | 2.331386291252311 | 0.008115697840013716 | 4.495757789852532
gi|150373376 | |dbj|BAF66636.1| conserved hypothetical protein | 6.508551479715034 | 0.7313324124915768 | 9.508471299869838 | 1.178457022869738 | 0.02000983161125404 | 1.460919734522274
[NAME] | |dbj|BAF66660.1| exotoxin NM1 | 42.7774066453159 | 2.937348804982667 | 109.9701786630157 | 12.63074335513859 | 0.0008530311791369828 | 2.570753752671853
gi|150373401 | |dbj|BAF66661.1| exotoxin NM2 | 19.53632692816125 | 3.124180931805955 | 43.2893380686813 | 8.019654597792591 | 0.008774507533688268 | 2.215838126985914
gi|150373403 | |dbj|BAF66663.1| exotoxin NM4 | 22.22554307843239 | 1.947839191034716 | 48.91134782028806 | 6.35119517133093 | 0.002242481682595416 | 2.200681785263169
gi|150373404 | [NAME] exotoxin NM5 | 6.48458728491974 | 1.067940549189862 | 19.0169425997397 | 2.356914045739476 | 0.001104809051951799 | 2.932637308154466
gi|150373406 | [NAME] exotoxin NM7 | 100.8634722731716 | 5.502510656140882 | 309.7243779046121 | 17.07694341233764 | 3.57135313395571e-05 | 3.070728886526693
gi|150373409 | |dbj|BAF66669.1| exotoxin NM10 | 16.43314848425809 | 1.310784952515455 | 20.37610292909261 | 1.011407091290138 | 0.01455349046329203 | 1.23993907488949
gi|150373412 | [NAME] exotoxin NM11 | 8.561360979120275 | 0.7730987799103646 | 12.88571863490741 | 0.7710793862892245 | 0.002364817542888809 | 1.505101661562164
gi|150373514 | [NAME] 50S ribosomal protein L7 | 1.715978223685078 | 0.6118057645208506 | 9.536791280448789 | 1.454410733153501 | 0.001011277181744345 | 5.557641203609476
gi|150373520 | |dbj|BAF66780.1| 30S ribosomal protein S7 | 2.392259991888395 | 0.5753802027452452 | 7.142431967026352 | 1.144094893480524 | 0.003017770486135603 | 2.985642025216616
gi|150373753 | |dbj|BAF67013.1| glyceraldehyde 3-phosphate dehydrogenase 1 | 3.779460212106474 | 1.275176862562681 | 13.98738857172075 | 4.25227587999522 | 0.01636419190657038 | 3.700895838753892
gi|150373755 | |dbj|BAF67015.1| triosephosphate isomerase | 2.376349069346267 | 1.524449661940729 | 19.4172058893529 | 3.295138287868056 | 0.0012454062031055 | 8.171024257263083
gi|150373770 | |dbj|BAF67030.1| extracellular matrix and plasma binding protein | 96.18247109690067 | 5.127269771582084 | 108.0055756139643 | 2.473650218499564 | 0.02281452799425199 | 1.122923692666954
gi|150373961 | |dbj|BAF67221.1| histidine-containing phosphocarrier protein | 4.45574198030979 | 0.6896242837602813 | 22.7359792656051 | 1.072015474440787 | 1.559202776467595e-05 | 5.102624740408409
gi|150374069 | |dbj|BAF67329.1| thioredoxin | 4.105618998810486 | 0.08448882919355775 | 12.57408328228597 | 2.225658674357964 | 0.002752963950153798 | 3.062652254368718
gi|150374079 | |dbj|BAF67339.1| formyl peptide receptor-like 1 inhibitory protein | 25.66555279805571 | 2.824849750037904 | 48.4002996094351 | 9.058264896514137 | 0.01425980309282899 | 1.885807798112248
gi|150374081 | |dbj|BAF67341.1| conserved hypothetical protein | 32.8874463245843 | 4.279972099008154 | 47.50853452588782 | 1.157752809991331 | 0.00464702027147026 | 1.444579614269833
gi|150374160 | |dbj|BAF67420.1| 30S ribosomal protein S16 | 2.052809499405243 | 0.04224441459677152 | 8.161636963178907 | 1.162442424592986 | 0.0008100336116677296 | 3.975837487864103
gi|150374179 | |dbj|BAF67439.1| elongation factor Ts (EF-Ts) | 8.866174787791982 | 2.457789482528752 | 26.2621624940473 | 6.412313094351165 | 0.011804429653912 | 2.962062346233937
gi|150374325 | |dbj|BAF67585.1| major cold-shock protein CspA | 2.052809499405243 | 0.04224441459677152 | 13.03898352701557 | 6.305837656912007 | 0.03925764720869206 | 6.35177474129642
gi|150374394 | |dbj|BAF67654.1| DNA-binding protein [NAME] | 4.116291487826638 | 1.10027908531478 | 12.86972464699046 | 2.079167058630325 | 0.002982164920911104 | 3.126533843643214
gi|150375055 | |dbj|BAF68315.1| conserved hypothetical protein | 1.713359006922091 | 0.6032135811637662 | 5.773440646087472 | 0.5944197347999641 | 0.001148647959300536 | 3.36966194636522
gi|150375098 | |dbj|BAF68358.1| alkaline shock protein 23 | 4.808484178572082 | 1.653664125628624 | 9.494311309580361 | 2.012649110593887 | 0.03567343104514564 | 1.974491535584042
gi|150375146 | |dbj|BAF68406.1| 50S ribosomal protein L30 | 2.052809499405243 | 0.04224441459677152 | 7.783530657813631 | 2.409896082059815 | 0.0146341910021 | 3.791647817329733
gi|150375159 | |dbj|BAF68419.1| 50S ribosomal protein L22 | 2.052809499405243 | 0.04224441459677152 | 8.831055634545137 | 0.620778200169204 | 4.646323014076019e-05 | 4.301936266908228
gi|150375327 | |dbj|BAF68587.1| 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | 2.052809499405243 | 0.04224441459677152 | 25.132172576655 | 2.390838932834964 | 7.50233156658896e-05 | 12.24281774998435
gi|150375511 | [NAME] dehydrogenase 2 | 2.066101205184383 | 1.070609267455057 | 5.781437640045945 | 1.625122522841972 | 0.02974546612366476 | 2.798235452135075
gi|151220271 | |ref|YP_001331094.1| siderophore compound ABC transporter binding protein | 2.739763756624713 | 0.6117993334448049 | 9.233152921785829 | 3.820778819976826 | 0.04382785527973661 | 3.370054406866354
gi|151220605 | |ref|YP_001331427.1| exotoxin NM6 | 6.5377541080363 | 3.251415766669826 | 20.44257388183646 | 3.3252405491202 | 0.006612900329576802 | 3.126849609823678
gi|150373053 | |dbj|BAF66313.1| conserved hypothetical protein | 55.85049126658973 | 7.223355268819253 | 31.22341157169491 | 0.7059299343105263 | 0.004187695693332151 | 0.559053481242573
[NAME] | |dbj|BAF66438.1| coagulase precursor | 29.47402001651925 | 5.49344403606542 | 18.71763323978024 | 2.150951699881132 | 0.03425044935331416 | 0.6350553208992057
gi|150373261 | |dbj|BAF66521.1| 5'-nucleotidase | 148.2761796780023 | 12.93361669815464 | 119.0104781473783 | 10.77773441790676 | 0.03951690169072387 | 0.8026270868714207
gi|150373274 | |dbj|BAF66534.1| truncated triacylglycerol lipase precursor | 53.78196646660657 | 8.609014911610979 | 33.17686162226317 | 5.842202096641583 | 0.02652901606523402 | 0.6168770649705196
gi|150373414 | |dbj|BAF66674.1| conserved hypothetical protein | 9.577093239806745 | 0.5549849675432533 | 5.073867996514823 | 0.8511832483072301 | 0.001548831731009611 | 0.5297920641960053
gi|150373441 | |dbj|BAF66701.1| N-acetylmuramoyl-L-alanine amidase aaa precursor | 7.208797442713643 | 1.94395691867138 | 2.024250002015633 | 0.9638080990555937 | 0.01439236579552343 | 0.2808027300117389
gi|150373597 | |dbj|BAF66857.1| conserved hypothetical protein | 103.0246477696803 | 13.83695595709012 | 64.20008376602497 | 4.025818772521615 | 0.009543900808865803 | 0.6231526644919896
gi|150373646 | |dbj|BAF66906.1| secretory antigen SsaA-like protein | 14.73570039987813 | 2.386189405750485 | 8.11732299468301 | 1.484628502390148 | 0.01511090574809269 | 0.5508610228496598
gi|150373768 | |dbj|BAF67028.1| clumping factor A | 3.418664741591016 | 0.5701684437248558 | 0.6552586810767537 | 1.134941327725508 | 0.01963067370658155 | 0.191670938979469
gi|150373934 | |dbj|BAF67194.1| bifunctional autolysin precursor | 88.35055870910027 | 7.925492411488895 | 60.81234443595287 | 3.891122645163768 | 0.005683602387776476 | 0.68830741225057
gi|150374078 | |dbj|BAF67338.1| conserved hypothetical protein | 16.77521819350423 | 1.427252113112418 | 7.447904323316781 | 1.349807911264083 | 0.001191784510508239 | 0.4439825603103504
gi|150374085 | |dbj|BAF67345.1| alpha-hemolysin precursor | 40.73526963492682 | 2.971969512211345 | 2.691834675754393 | 2.573051301740144 | 7.423206141675377e-05 | 0.06608117977072105
gi|150374089 | [NAME] conserved hypothetical protein | 4.458361197072778 | 1.238303791805821 | 1.346834336690931 | 0.5361534471335606 | 0.01621229204323213 | 0.3020917949795591
gi|150374717 | |dbj|BAF67977.1| serine protease SplB | 6.142517575673601 | 1.683234588528453 | 2.052569982594584 | 1.047370748839264 | 0.02330717755836568 | 0.3341577711919685
gi|150374730 | |dbj|BAF67990.1| leukocidin LukD precursor | 82.10452232592074 | 3.197879377362662 | 30.21036957187336 | 2.325593431352798 | 2.218391774492742e-05 | 0.3679501288851153
gi|150374731 | |dbj|BAF67991.1| [NAME] precursor | 68.10069411216129 | 3.8451741453707 | 35.17384907162575 | 10.02777382534955 | 0.006045042570814122 | 0.51649765880058
[NAME] | |dbj|BAF68199.1| leukocidin/hemolysin toxin family F subunit | 131.7087815873668 | 3.860069557725605 | 45.20713214816072 | 3.9688612113407 | 1.108627187017435e-05 | 0.3432355200869682
gi|150374940 | |dbj|BAF68200.1| leukocidin/hemolysin toxin family S subunit | 243.4472156430848 | 20.75358841956404 | 102.9686855954355 | 12.3419631061812 | 0.0005455949855968529 | 0.4229610321212166
gi|150375329 | |dbj|BAF68589.1| immunoglobulin G-binding protein Sbi | 51.65706876085124 | 0.8073664453871837 | 23.12208256492884 | 1.846315661834722 | 1.639860247228852e-05 | 0.4476073288628432
gi|150375330 | |dbj|BAF68590.1| gamma-hemolysin component A | 103.7174273263183 | 5.861405095099471 | 61.65535098604774 | 9.42487529659713 | 0.002786633329938548 | 0.5944550744791052
gi|150375331 | |dbj|BAF68591.1| gamma-hemolysin component C | 120.3891267123166 | 5.125672489232089 | 61.93683236046275 | 7.784258134056463 | 0.0004076306632516185 | 0.5144719797533525
gi|150375332 | |dbj|BAF68592.1| gamma hemolysin | 127.3992483706275 | 11.82804186012343 | 60.95394433884763 | 9.466817699036334 | 0.001611114481686683 | 0.4784482257031969
gi|150375411 | |dbj|BAF68671.1| fibronectin binding protein A precursor | 2.72909126760856 | 0.544297618324446 | 1.368991320938879 | 0.6323186304772345 | 0.04765463393657245 | 0.5016289990691645
gi|150375555 | |dbj|BAF68815.1| N-acetylmuramoyl-L-alanine amidase domain protein | 7.550867151959781 | 2.260140641345462 | 1.027201990111027 | 1.050060499647811 | 0.01054559636128161 | 0.1360376191818476
gi|150375581 | |dbj|BAF68841.1| lipase precursor | 36.29543857715916 | 4.305061807902696 | 25.77760026614581 | 1.423610238789816 | 0.01589523137446398 | 0.7102159741463419
gi|151220614 | |ref|YP_001331436.1| conserved hypothetical protein | 9.577093239806745 | 0.5549849675432533 | 5.073867996514823 | 0.8511832483072301 | 0.001548831731009611 | 0.5297920641960053
gi|151221917 | |ref|YP_001332739.1| serine protease SplB | 6.142517575673601 | 1.683234588528453 | 2.052569982594584 | 1.047370748839264 | 0.02330717755836568 | 0.3341577711919685
gi|151222321 | |ref|YP_001333143.1| truncated MHC class II analog protein | 52.69133116084282 | 2.841878060808183 | 13.91908362134943 | 0.3822865069333359 | 1.970439629331395e-05 | 0.2641626870055866
r-gi|150374378 |  | 3.065922543328723 | 0.9706643429815582 | 1.019204996152554 | 0.03366130987499 | 0.02177217717024163 | 0.3324301190746934
1 Protein ID in NCBI for S. aureus strain Newman.
2 Protein function based on BLAST searches for S. aureus strain Newman.
3 Mean of the number of spectra detected in the three samples analyzed for this condition.
4 Standard deviation among the number of the spectra detected in the three samples analyzed for this condition.
5 p-value as obtained by applying Student's t-test.
6 Ratio of the mean of number of spectra detected in the presence of gramicidin to that in its absence.