Spreadsheet: 13a0aa53-ec5d-4d2b-a957-cf1db4bb0453
Sheet: Sheet1

Unnamed: 0 | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | In-frame to out-of-frame ratio | Unnamed: 11 | Unnamed: 12 | Most common disruptions induced by X4-ZFNs3 | Unnamed: 14 | Unnamed: 15 | Unnamed: 16 | Unnamed: 17 | Unnamed: 18 | Unnamed: 19
CCR5 genotype | Donors | # Reads | Day1 | Ad5/F35 X4-ZFN MOI | HIV | %NHEJ | %Insertions | %Deletions | %Multiple2 | Total | Deletions | Insertions | CXCR4Δ25 | [NAME] | CXCR4Δ15 | CXCR4Δ12 | CXCR4Δ9 | CXCR4Δ2 | CXCR4+4
wt/wt | ND306 | 4940 | 5 | 300 | Mock | 10.8 | 1.6 | 8.4 | 0.7 | 0.93 | 1.29 | 0.09 | 0.01773 | 0.09453 | 0.0384 | 0.02954 | 0.0517 | 0.00295 | 0.0192
 |  | 4431 | 5 | 600 | Mock | 23.6 | 3.8 | 18.3 | 1.3 | 0.89 | 1.25 | 0.05 | 0.01178 | 0.12047 | 0.05163 | 0.00996 | 0.05163 | 0.01812 | 0.01812
 |  | 4772 | 5 | 1000 | Mock | 28.3 | 4.8 | 21.6 | 1.9 | 1 | 1.44 | 0.11 | 0.01897 | 0.09105 | 0.0569 | 0.02959 | 0.05008 | 0.02049 | 0.02959
wt/wt | ND335 | 4801 | 13 | 600 | Mock | 24.3 | 4.1 | 18.3 | 1.8 | 0.88 | 1.27 | 0.08 | 0.01347 | 0.11886 | 0.05784 | 0.0317 | 0.05784 | 0.01426 | 0.03487
 |  | 5080 | 13 | 600 | Bk132 | 42.1 | 6.9 | 32.7 | 2.5 | 0.92 | 1.31 | 0.08 | 0.01606 | 0.13342 | 0.05087 | 0.03213 | 0.06247 | 0.01249 | 0.03793
 |  | 5108 | 13 | 600 | HxB2 | 29 | 4.2 | 22.5 | 2.3 | 0.87 | 1.14 | 0.12 | 0.01451 | 0.13565 | 0.05174 | 0.02587 | 0.03785 | 0.01073 | 0.02776
 |  | 4902 | 13 | 600 | [NAME] (1) | 38.2 | 5.3 | 30.1 | 2.8 | 0.94 | 1.27 | 0.07 | 0.01778 | 0.1356 | 0.05688 | 0.02895 | 0.0452 | 0.00914 | 0.03047
 |  | 4546 | 13 | 600 | [NAME] (2) | 40.9 | 5.2 | 33 | 2.6 | 1.07 | 1.42 | 0.09 | 0.01486 | 0.14139 | 0.0707 | 0.03074 | 0.07377 | 0.01537 | 0.02818
wt/wt | ND221 | 3261 | 11 | 600 | Mock | 19.6 | 2.8 | 15.6 | 1 | 1.08 | 1.42 | 0.2 | 0.01499 | 0.11853 | 0.04496 | 0.0327 | 0.05858 | 0.0109 | 0.01771
 |  | 3979 | 18 | 600 | Mock | 19 | 2.8 | 15.4 | 0.8 | 0.98 | 1.39 | 0.11 | 0.00343 | 0.12815 | 0.06407 | 0.02746 | 0.04805 | 0.0103 | 0.02975
 |  | 3503 | 24 | 600 | Mock | 17.9 | 2.5 | 14.3 | 0.9 | 1.09 | 1.53 | 0.16 | 0.00412 | 0.14952 | 0.04527 | 0.03155 | 0.05075 | 0.01509 | 0.02332
 |  | 3291 | 11 | 600 | Bk132 | 18.1 | 2.5 | 14.8 | 0.8 | 0.92 | 1.23 | 0.08 | 0.01445 | 0.13873 | 0.05202 | 0.03468 | 0.0578 | 0.01156 | 0.03468
 |  | 2832 | 18 | 600 | Bk132 | 70.2 | 9.6 | 57.3 | 3.3 | 1.05 | 1.44 | 0.07 | 0.01835 | 0.14872 | 0.06567 | 0.0338 | 0.07098 | 0.01014 | 0.0338
 |  | 2475 | 24 | 600 | Bk132 | 83.3 | 12.1 | 67.5 | 3.6 | 1.16 | 1.62 | 0.11 | 0.01265 | 0.15558 | 0.06935 | 0.0403 | 0.07357 | 0.01874 | 0.0314
 |  | 2997 | 11 | 600 | HxB2 | 20.2 | 3.7 | 15.6 | 0.9 | 0.94 | 1.46 | 0.07 | 0.01156 | 0.11994 | 0.0448 | 0.03468 | 0.06792 | 0.01156 | 0.03613
 |  | 2839 | 18 | 600 | HxB2 | 48.9 | 6.9 | 39.4 | 2.7 | 1.04 | 1.45 | 0.07 | 0.00815 | 0.13655 | 0.05774 | 0.0394 | 0.06861 | 0.01019 | 0.0197
 |  | 3070 | 24 | 600 | HxB2 | 61.9 | 7.7 | 51.1 | 3.1 | 1.12 | 1.51 | 0.09 | 0.01458 | 0.14228 | 0.06737 | 0.04274 | 0.06536 | 0.01257 | 0.02011
 |  | 3640 | 11 | 600 | R3A | 21.5 | 2.6 | 17.9 | 1 | 1.06 | 1.44 | 0.09 | 0.00338 | 0.1295 | 0.05856 | 0.02815 | 0.06194 | 0.00901 | 0.02477
 |  | 2784 | 18 | 600 | R3A | 61.2 | 7.7 | 50 | 3.5 | 1.18 | 1.62 | 0.1 | 0.01064 | 0.15574 | 0.06667 | 0.04202 | 0.05994 | 0.00616 | 0.03137
 |  | 2793 | 24 | 600 | R3A | 75.4 | 9.5 | 62.4 | 3.5 | 1.21 | 1.63 | 0.11 | 0.01188 | 0.16773 | 0.05667 | 0.03976 | 0.07038 | 0.00868 | 0.01965
wt/wt | ND336 | 734 | 5 | 600 | Mock | 38.1 | 5.6 | 30.6 | 1.9 | 0.84 | 1.09 | 0.13 | 0.04153 | 0.10224 | 0.04473 | 0.01597 | 0.02875 | 0.00958 | 0.02556
 |  | 1924 | 16 | 600 | Mock | 28.8 | 3.7 | 23.2 | 1.8 | 0.74 | 0.94 | 0.09 | 0.03438 | 0.08438 | 0.07187 | 0.01875 | 0.01875 | 0.01094 | 0.02813
 |  | 1827 | 26 | 600 | Mock | 29.3 | 3.7 | 23.8 | 1.8 | 0.71 | 0.95 | 0 | 0.02663 | 0.10355 | 0.05128 | 0.02564 | 0.03057 | 0.01381 | 0.03057
 |  | 1636 | 16 | 600 | Bk132 | 58.6 | 7.2 | 48.2 | 3.2 | 0.68 | 0.83 | 0.09 | 0.02564 | 0.08974 | 0.03846 | 0.02244 | 0.03526 | 0.01603 | 0.02885
 |  | 1587 | 26 | 600 | Bk132 | 87.3 | 8.6 | 74.9 | 3.7 | 0.74 | 0.88 | 0.08 | 0.03297 | 0.08571 | 0.05275 | 0.03297 | 0.03297 | 0.01538 | 0.00879
 |  | 704 | 16 | 600 | HxB2 | 40.4 | 5.8 | 32.2 | 2.3 | 0.81 | 1.02 | 0.19 | 0.01927 | 0.12661 | 0.04679 | 0.01284 | 0.03028 | 0.01193 | 0.02752
 |  | 1294 | 26 | 600 | HxB2 | 91.3 | 10.5 | 77.2 | 3.5 | 0.85 | 1.05 | 0.06 | 0.021 | 0.10824 | 0.05654 | 0.01939 | 0.02585 | 0.01292 | 0.02423
 |  | 922 | 16 | 600 | R3A | 44.4 | 3.4 | 40 | 1 | 0.72 | 0.79 | 0.16 | 0.02958 | 0.12254 | 0.05493 | 0.02042 | 0.02887 | 0.00915 | 0.02535
 |  | 1887 | 26 | 600 | R3A | 88.2 | 7.6 | 75.7 | 4.8 | 0.8 | 0.97 | 0.05 | 0.02869 | 0.12623 | 0.05574 | 0.03689 | 0.02459 | 0.01475 | 0.02541
 |  | 2666 | 16 | 600 | Tybe | 36.8 | 4.5 | 30.3 | 2 | 0.75 | 0.93 | 0.1 | 0.02055 | 0.12155 | 0.06283 | 0.02114 | 0.02701 | 0.02055 | 0.02877
 |  | 2929 | 26 | 600 | Tybe | 92.2 | 7.1 | 80.3 | 4.7 | 0.87 | 1.02 | 0.05 | 0.02424 | 0.13242 | 0.07453 | 0.02677 | 0.02279 | 0.0123 | 0.02822
Δ32/Δ32 | [NAME] | 2720 | 5 | 600 | Mock | 32.9 | 5.9 | 25.3 | 1.7 | 0.77 | 1.18 | 0.05 | 0.0159 | 0.1004 | 0.04076 | 0.02684 | 0.04573 | 0.0169 | 0.01193
 |  | 2583 | 13 | 600 | Mock | 31.8 | 5.1 | 24.9 | 1.9 | 0.68 | 0.98 | 0.1 | 0.0097 | 0.11315 | 0.04095 | 0.02909 | 0.03233 | 0.01509 | 0.02694
 |  | 2341 | 26 | 600 | Mock | 32.7 | 4.7 | 26.7 | 1.4 | 0.78 | 1.08 | 0.04 | 0.01161 | 0.11847 | 0.04297 | 0.02787 | 0.03833 | 0.01858 | 0.02207
 |  | 2035 | 13 | 600 | Bk132 | 42.3 | 7.7 | 33 | 1.6 | 0.69 | 1 | 0.06 | 0.00952 | 0.12063 | 0.04868 | 0.02751 | 0.03175 | 0.00635 | 0.02646
 |  | 2105 | 26 | 600 | Bk132 | 89.2 | 13.4 | 72.4 | 3.4 | 0.86 | 1.19 | 0.08 | 0.01731 | 0.14155 | 0.06976 | 0.02851 | 0.04379 | 0.0112 | 0.02648
 |  | 2398 | 13 | 600 | HxB2 | 31.7 | 4.1 | 26 | 1.7 | 0.84 | 1.1 | 0.14 | 0.01397 | 0.12456 | 0.05588 | 0.02328 | 0.04191 | 0.00698 | 0.02445
 |  | 4416 | 26 | 600 | HxB2 | 83.1 | 10.6 | 68.3 | 4.1 | 0.89 | 0.83 | -0.04 | 0.0186 | 0.13049 | 0.04787 | 0.02597 | 0.04424 | 0.01315 | 0.02116
 |  | 2165 | 13 | 600 | R3A | 58 | 8.9 | 46 | 3 | 0.81 | 1.16 | 0.06 | 0.01786 | 0.11756 | 0.05208 | 0.02976 | 0.04836 | 0.01488 | 0.02455
 |  | 1686 | 26 | 600 | R3A | 89.7 | 13.2 | 72.6 | 3.7 | 0.83 | 1.16 | 0.07 | 0.02087 | 0.15054 | 0.04997 | 0.03163 | 0.04428 | 0.01518 | 0.03542
1Days refers to days post stimulation. Cells were transduced with the X4-ZFNson day 1 and HIV infected on day 5.
2Multiple refers to sequences with multiple insertions and deletions.
3Refers to disruptions shown in Figure 1B.