Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - December 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 184
Report Period
Issued 10 January 2022

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
December
2021
%
Total 2021
%
Submissions
238
3952
Samples
Total (%)
13117
100.00 %
293371
100.00 %
England
10441
79.60 %
227569
77.57 %
Wales
2676
20.40 %
63779
21.74 %
Scotland
2023
0.69 %
Parallel Tests
PAR-CUL-N
987
7.52 %
110595
37.70 %
PAR-CUL-P
90
0.69 %
12040
4.10 %
PAR-HERD-S
0
0.00 %
1633
0.56 %
PAR-IR-EX
24
0.18 %
449
0.15 %
PAR-IR-SEV
274
2.09 %
2885
0.98 %
PAR-NEW-IN
1948
14.85 %
79242
27.01 %
PAR-OTHER
849
6.47 %
10097
3.44 %
PAR-PERSIS
2480
18.91 %
39239
13.38 %
PAR-POSTM
61
0.47 %
342
0.12 %
PAR-RAPID
65
0.50 %
1146
0.39 %
PAR-RECUR
5443
41.50 %
28586
9.74 %
Total(% of all samples)
12221
93.17 %
286254
97.57 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
26
0.01 %
SER-FLEXI
892
6.80 %
7024
2.39 %
SER-FRAUD
0
0.00 %
SER-NSR
4
0.03 %
8
0.00 %
SER-OTHER
0
0.00 %
59
0.02 %
Total(% of all samples)
896
6.83 %
7117
2.43 %
Total
13117
100.00 %
293371
100.00 %
Retests
Total (% of all samples)
979
7.46 %
21488
7.32 %
England
753
5.74 %
17074
5.82 %
Wales
226
1.72 %
4253
1.45 %
Scotland
161
0.05 %
Resamples
Total (% of all samples)
852
6.50 %
17680
6.03 %
England
657
5.01 %
14147
4.82 %
Wales
195
1.49 %
3396
1.16 %
Scotland
137
0.05 %
Rejects
Total (% of all samples)
29
0.22 %
923
0.31 %
England
22
0.17 %
751
0.26 %
Wales
7
0.05 %
160
0.05 %
Scotland
0
0.00 %
12
0.00 %
Table 1. Monthly Totals for December 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CUL-N
987
7.5 %
PAR-CUL-P
90
0.7 %
PAR-IR-EX
24
0.2 %
PAR-IR-SEV
274
2.1 %
PAR-NEW-IN
1948
14.9 %
PAR-OTHER
849
6.5 %
PAR-PERSIS
2480
18.9 %
PAR-POSTM
61
0.5 %
PAR-RAPID
65
0.5 %
PAR-RECUR
5443
41.5 %
SER-FLEXI
892
6.8 %
SER-NSR
4
0.0 %
Total
13117
100.00 %
Figure 1. Number of samples submitted in each category during December 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
Lincs
Lancashire
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Avon
Wales
England
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during December 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
Wigtown
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
3
187
1.43 %
13
6.95 %
164
87.70 %
10
5.35 %
9
4.81 %
1
0.53 %
PAR-PERSIS
1
85
0.65 %
0
0.00 %
80
94.12 %
6
7.06 %
5
5.88 %
0
0.00 %
PAR-RECUR
2
53
0.40 %
3
5.66 %
38
71.70 %
4
7.55 %
12
22.64 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
2
87
0.66 %
1
1.15 %
84
96.55 %
4
4.60 %
2
2.30 %
0
0.00 %
PAR-PERSIS
3
786
5.99 %
30
3.82 %
684
87.02 %
79
10.05 %
71
9.03 %
1
0.13 %
PAR-RECUR
8
1,434
10.93 %
71
4.95 %
1294
90.24 %
74
5.16 %
56
3.91 %
13
0.91 %
Derbyshire
PAR-RECUR
1
29
0.22 %
0
0.00 %
17
58.62 %
12
41.38 %
12
41.38 %
0
0.00 %
Devon
PAR-CUL-P
1
26
0.20 %
1
3.85 %
24
92.31 %
1
3.85 %
1
3.85 %
0
0.00 %
PAR-NEW-IN
1
22
0.17 %
1
4.55 %
10
45.45 %
11
50.00 %
11
50.00 %
0
0.00 %
PAR-PERSIS
5
876
6.68 %
49
5.59 %
767
87.56 %
74
8.45 %
58
6.62 %
2
0.23 %
PAR-RECUR
7
1,145
8.73 %
30
2.62 %
1061
92.66 %
67
5.85 %
54
4.72 %
0
0.00 %
Dorset
PAR-CUL-N
1
14
0.11 %
0
0.00 %
6
42.86 %
10
71.43 %
8
57.14 %
0
0.00 %
PAR-OTHER
1
4
0.03 %
0
0.00 %
2
50.00 %
3
75.00 %
2
50.00 %
0
0.00 %
PAR-RECUR
2
247
1.88 %
39
15.79 %
202
81.78 %
6
2.43 %
6
2.43 %
0
0.00 %
Gloucs
PAR-CUL-N
3
298
2.27 %
46
15.44 %
231
77.52 %
28
9.40 %
21
7.05 %
0
0.00 %
PAR-PERSIS
1
14
0.11 %
3
21.43 %
11
78.57 %
1
7.14 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
2
0.02 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Heref
PAR-OTHER
2
21
0.16 %
3
14.29 %
17
80.95 %
4
19.05 %
1
4.76 %
0
0.00 %
PAR-RECUR
5
778
5.93 %
38
4.88 %
710
91.26 %
38
4.88 %
30
3.86 %
0
0.00 %
Shropshire
PAR-PERSIS
3
353
2.69 %
12
3.40 %
307
86.97 %
38
10.76 %
34
9.63 %
0
0.00 %
PAR-RECUR
4
323
2.46 %
18
5.57 %
280
86.69 %
25
7.74 %
25
7.74 %
0
0.00 %
Somerset excl N
PAR-CUL-N
6
564
4.30 %
16
2.84 %
512
90.78 %
37
6.56 %
36
6.38 %
0
0.00 %
PAR-CUL-P
2
64
0.49 %
4
6.25 %
45
70.31 %
17
26.56 %
15
23.44 %
0
0.00 %
PAR-RECUR
9
521
3.97 %
61
11.71 %
441
84.64 %
19
3.65 %
18
3.45 %
1
0.19 %
Staffs
PAR-CUL-N
2
11
0.08 %
0
0.00 %
5
45.45 %
6
54.55 %
6
54.55 %
0
0.00 %
PAR-NEW-IN
4
381
2.90 %
12
3.15 %
354
92.91 %
20
5.25 %
15
3.94 %
0
0.00 %
Table 2a. Summary by County for December 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
5
580
4.42 %
25
4.31 %
519
89.48 %
47
8.10 %
36
6.21 %
0
0.00 %
Wiltshire
PAR-CUL-N
1
13
0.10 %
0
0.00 %
10
76.92 %
3
23.08 %
3
23.08 %
0
0.00 %
PAR-NEW-IN
1
146
1.11 %
9
6.16 %
118
80.82 %
12
8.22 %
15
10.27 %
4
2.74 %
PAR-OTHER
1
282
2.15 %
5
1.77 %
269
95.39 %
11
3.90 %
8
2.84 %
0
0.00 %
PAR-PERSIS
1
9
0.07 %
1
11.11 %
4
44.44 %
5
55.56 %
4
44.44 %
0
0.00 %
SER-FLEXI
1
3
0.02 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Bucks
PAR-NEW-IN
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
1
326
2.49 %
13
3.99 %
297
91.10 %
14
4.29 %
16
4.91 %
0
0.00 %
PAR-RECUR
1
15
0.11 %
0
0.00 %
12
80.00 %
4
26.67 %
3
20.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
291
2.22 %
5
1.72 %
258
88.66 %
32
11.00 %
28
9.62 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
42
0.32 %
1
2.38 %
39
92.86 %
3
7.14 %
2
4.76 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
57
0.43 %
3
5.26 %
32
56.14 %
10
17.54 %
22
38.60 %
0
0.00 %
Oxon
PAR-NEW-IN
1
26
0.20 %
3
11.54 %
22
84.62 %
1
3.85 %
1
3.85 %
0
0.00 %
PAR-PERSIS
2
318
2.42 %
10
3.14 %
299
94.03 %
15
4.72 %
9
2.83 %
0
0.00 %
England - LRA
Lancashire
SER-NSR
1
4
0.03 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Lincs
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
England
104
10441
79.60 %
527
5.05 %
9235
88.45 %
753
7.21 %
657
6.29 %
22
0.21 %
Wales - High TB
Carms
PAR-IR-SEV
6
31
0.24 %
11
35.48 %
18
58.06 %
3
9.68 %
2
6.45 %
0
0.00 %
PAR-OTHER
1
2
0.02 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
6
0.05 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
11
0.08 %
3
27.27 %
7
63.64 %
2
18.18 %
1
9.09 %
0
0.00 %
SER-FLEXI
2
279
2.13 %
26
9.32 %
237
84.95 %
20
7.17 %
16
5.73 %
0
0.00 %
Ceredigion
PAR-IR-SEV
4
23
0.18 %
4
17.39 %
18
78.26 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-RAPID
3
4
0.03 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
Gwent
PAR-IR-SEV
1
2
0.02 %
0
0.00 %
1
50.00 %
2
100.00 %
1
50.00 %
0
0.00 %
Pembs
PAR-IR-EX
7
20
0.15 %
6
30.00 %
14
70.00 %
2
10.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
13
83
0.63 %
30
36.14 %
51
61.45 %
2
2.41 %
1
1.20 %
1
1.20 %
PAR-PERSIS
3
26
0.20 %
0
0.00 %
22
84.62 %
5
19.23 %
4
15.38 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
15
18
0.14 %
4
22.22 %
13
72.22 %
1
5.56 %
1
5.56 %
0
0.00 %
PAR-RECUR
7
292
2.23 %
12
4.11 %
261
89.38 %
19
6.51 %
18
6.16 %
1
0.34 %
SER-FLEXI
5
601
4.58 %
51
8.49 %
510
84.86 %
37
6.16 %
36
5.99 %
4
0.67 %
Powys
PAR-IR-SEV
3
28
0.21 %
3
10.71 %
20
71.43 %
5
17.86 %
5
17.86 %
0
0.00 %
PAR-OTHER
2
224
1.71 %
10
4.46 %
193
86.16 %
22
9.82 %
21
9.38 %
0
0.00 %
PAR-PERSIS
2
5
0.04 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
5
0.04 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
24
0.18 %
0
0.00 %
19
79.17 %
5
20.83 %
5
20.83 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-EX
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
121
0.92 %
7
5.79 %
103
85.12 %
14
11.57 %
11
9.09 %
0
0.00 %
NE Wales
PAR-IR-SEV
11
107
0.82 %
15
14.02 %
92
85.98 %
1
0.93 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
331
2.52 %
13
3.93 %
290
87.61 %
33
9.97 %
28
8.46 %
0
0.00 %
PAR-OTHER
3
6
0.05 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-POSTM
6
32
0.24 %
3
9.38 %
26
81.25 %
3
9.38 %
3
9.38 %
0
0.00 %
PAR-RAPID
9
21
0.16 %
5
23.81 %
16
76.19 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
2
0.02 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
2
0.02 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
6
0.05 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
51
0.39 %
3
5.88 %
43
84.31 %
6
11.76 %
4
7.84 %
1
1.96 %
PAR-POSTM
3
23
0.18 %
1
4.35 %
21
91.30 %
1
4.35 %
1
4.35 %
0
0.00 %
NE Wales
PAR-NEW-IN
2
271
2.07 %
16
5.90 %
224
82.66 %
36
13.28 %
31
11.44 %
0
0.00 %
PAR-POSTM
2
6
0.05 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
9
0.07 %
1
11.11 %
6
66.67 %
2
22.22 %
2
22.22 %
0
0.00 %
Wales
134
2676
20.40 %
232
8.67 %
2242
83.78 %
226
8.45 %
195
7.29 %
7
0.26 %
Grand Total
Sum:
238
13117
759
11477
979
852
29
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
7
882
0.30 %
66
7.48 %
774
87.76 %
46
5.22 %
39
4.42 %
3
0.34 %
PAR-CUL-P
7
829
0.28 %
48
5.79 %
721
86.97 %
85
10.25 %
60
7.24 %
0
0.00 %
PAR-HERD-S
3
397
0.14 %
38
9.57 %
344
86.65 %
19
4.79 %
15
3.78 %
0
0.00 %
PAR-OTHER
9
511
0.17 %
47
9.20 %
422
82.58 %
54
10.57 %
40
7.83 %
2
0.39 %
PAR-PERSIS
13
1,764
0.60 %
211
11.96 %
1415
80.22 %
154
8.73 %
129
7.31 %
9
0.51 %
PAR-RECUR
3
54
0.02 %
3
5.56 %
39
72.22 %
4
7.41 %
12
22.22 %
0
0.00 %
SER-ANOM
3
26
0.01 %
0
0.00 %
22
84.62 %
3
11.54 %
3
11.54 %
1
3.85 %
Corn & Scilly
PAR-CUL-N
102
9,111
3.11 %
403
4.42 %
7874
86.42 %
893
9.80 %
748
8.21 %
86
0.94 %
PAR-CUL-P
19
3,572
1.22 %
282
7.89 %
3073
86.03 %
245
6.86 %
215
6.02 %
2
0.06 %
PAR-OTHER
6
652
0.22 %
83
12.73 %
514
78.83 %
40
6.13 %
31
4.75 %
24
3.68 %
PAR-PERSIS
13
1,993
0.68 %
71
3.56 %
1796
90.12 %
147
7.38 %
125
6.27 %
1
0.05 %
PAR-RECUR
16
2,587
0.88 %
159
6.15 %
2289
88.48 %
153
5.91 %
126
4.87 %
13
0.50 %
SER-FLEXI
4
604
0.21 %
30
4.97 %
552
91.39 %
27
4.47 %
22
3.64 %
0
0.00 %
SER-OTHER
1
7
0.00 %
0
0.00 %
6
85.71 %
3
42.86 %
1
14.29 %
0
0.00 %
Derbyshire
PAR-RECUR
2
544
0.19 %
10
1.84 %
493
90.63 %
42
7.72 %
41
7.54 %
0
0.00 %
Devon
PAR-CUL-N
348
37,178
12.67 %
2282
6.14 %
32670
87.87 %
2532
6.81 %
2078
5.59 %
148
0.40 %
PAR-CUL-P
16
1,581
0.54 %
90
5.69 %
1361
86.08 %
161
10.18 %
126
7.97 %
4
0.25 %
PAR-NEW-IN
13
1,025
0.35 %
64
6.24 %
852
83.12 %
109
10.63 %
106
10.34 %
3
0.29 %
PAR-OTHER
5
365
0.12 %
27
7.40 %
322
88.22 %
20
5.48 %
16
4.38 %
0
0.00 %
PAR-PERSIS
24
4,118
1.40 %
182
4.42 %
3704
89.95 %
299
7.26 %
227
5.51 %
5
0.12 %
PAR-RECUR
27
4,560
1.55 %
131
2.87 %
4147
90.94 %
337
7.39 %
280
6.14 %
2
0.04 %
SER-FLEXI
5
117
0.04 %
13
11.11 %
99
84.62 %
7
5.98 %
5
4.27 %
0
0.00 %
Dorset
PAR-CUL-N
76
9,417
3.21 %
576
6.12 %
8370
88.88 %
595
6.32 %
466
4.95 %
5
0.05 %
PAR-CUL-P
4
581
0.20 %
56
9.64 %
511
87.95 %
18
3.10 %
14
2.41 %
0
0.00 %
PAR-OTHER
4
148
0.05 %
8
5.41 %
133
89.86 %
10
6.76 %
7
4.73 %
0
0.00 %
PAR-PERSIS
2
405
0.14 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
247
0.08 %
39
15.79 %
202
81.78 %
6
2.43 %
6
2.43 %
0
0.00 %
Gloucs
PAR-CUL-N
88
8,709
2.97 %
448
5.14 %
7575
86.98 %
781
8.97 %
649
7.45 %
37
0.42 %
PAR-CUL-P
2
189
0.06 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
PAR-OTHER
1
66
0.02 %
2
3.03 %
62
93.94 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-PERSIS
11
2,265
0.77 %
183
8.08 %
1927
85.08 %
226
9.98 %
151
6.67 %
4
0.18 %
PAR-RECUR
4
254
0.09 %
5
1.97 %
233
91.73 %
18
7.09 %
14
5.51 %
2
0.79 %
Heref
PAR-CUL-N
15
954
0.33 %
48
5.03 %
801
83.96 %
87
9.12 %
79
8.28 %
26
2.73 %
PAR-CUL-P
1
271
0.09 %
18
6.64 %
243
89.67 %
15
5.54 %
10
3.69 %
0
0.00 %
PAR-OTHER
7
747
0.25 %
33
4.42 %
644
86.21 %
78
10.44 %
70
9.37 %
0
0.00 %
PAR-PERSIS
7
1,049
0.36 %
38
3.62 %
572
54.53 %
452
43.09 %
439
41.85 %
0
0.00 %
PAR-RECUR
20
2,522
0.86 %
150
5.95 %
2259
89.57 %
133
5.27 %
112
4.44 %
1
0.04 %
SER-OTHER
1
52
0.02 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
27
4,557
1.55 %
175
3.84 %
4144
90.94 %
253
5.55 %
220
4.83 %
18
0.39 %
PAR-NEW-IN
2
233
0.08 %
13
5.58 %
212
90.99 %
10
4.29 %
8
3.43 %
0
0.00 %
PAR-OTHER
9
1,050
0.36 %
63
6.00 %
950
90.48 %
42
4.00 %
29
2.76 %
8
0.76 %
PAR-PERSIS
4
595
0.20 %
31
5.21 %
518
87.06 %
53
8.91 %
46
7.73 %
0
0.00 %
PAR-RECUR
23
2,745
0.94 %
135
4.92 %
2467
89.87 %
169
6.16 %
140
5.10 %
3
0.11 %
Somerset excl N
PAR-CUL-N
117
12,649
4.31 %
782
6.18 %
11322
89.51 %
697
5.51 %
530
4.19 %
15
0.12 %
PAR-CUL-P
21
3,020
1.03 %
122
4.04 %
2758
91.32 %
161
5.33 %
127
4.21 %
13
0.43 %
PAR-NEW-IN
2
370
0.13 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.16 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
6
969
0.33 %
35
3.61 %
892
92.05 %
59
6.09 %
40
4.13 %
2
0.21 %
PAR-RECUR
26
3,158
1.08 %
167
5.29 %
2844
90.06 %
183
5.79 %
145
4.59 %
2
0.06 %
SER-FLEXI
3
55
0.02 %
9
16.36 %
42
76.36 %
2
3.64 %
2
3.64 %
2
3.64 %
Staffs
PAR-CUL-N
82
11,523
3.93 %
410
3.56 %
10373
90.02 %
865
7.51 %
738
6.40 %
2
0.02 %
PAR-CUL-P
5
471
0.16 %
42
8.92 %
406
86.20 %
39
8.28 %
23
4.88 %
0
0.00 %
PAR-NEW-IN
7
425
0.14 %
14
3.29 %
390
91.76 %
27
6.35 %
21
4.94 %
0
0.00 %
PAR-OTHER
2
188
0.06 %
5
2.66 %
157
83.51 %
25
13.30 %
26
13.83 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
3
645
0.22 %
15
2.33 %
583
90.39 %
52
8.06 %
46
7.13 %
1
0.16 %
PAR-RECUR
13
1,863
0.64 %
80
4.29 %
1655
88.84 %
151
8.11 %
128
6.87 %
0
0.00 %
Wiltshire
PAR-CUL-N
40
4,027
1.37 %
301
7.47 %
3436
85.32 %
339
8.42 %
281
6.98 %
9
0.22 %
PAR-CUL-P
13
1,096
0.37 %
40
3.65 %
988
90.15 %
62
5.66 %
49
4.47 %
19
1.73 %
PAR-NEW-IN
3
337
0.11 %
35
10.39 %
270
80.12 %
27
8.01 %
28
8.31 %
4
1.19 %
PAR-OTHER
1
282
0.10 %
5
1.77 %
269
95.39 %
11
3.90 %
8
2.84 %
0
0.00 %
PAR-PERSIS
12
1,760
0.60 %
77
4.38 %
1577
89.60 %
120
6.82 %
99
5.63 %
7
0.40 %
PAR-RECUR
3
439
0.15 %
11
2.51 %
403
91.80 %
27
6.15 %
23
5.24 %
2
0.46 %
SER-FLEXI
4
225
0.08 %
23
10.22 %
188
83.56 %
4
1.78 %
4
1.78 %
10
4.44 %
Worcs
PAR-CUL-N
2
128
0.04 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
PAR-PERSIS
3
323
0.11 %
45
13.93 %
263
81.42 %
16
4.95 %
15
4.64 %
0
0.00 %
England - Edge
Berks
PAR-CUL-P
2
319
0.11 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
20
1,480
0.50 %
83
5.61 %
1296
87.57 %
112
7.57 %
101
6.82 %
0
0.00 %
PAR-PERSIS
4
247
0.08 %
9
3.64 %
234
94.74 %
7
2.83 %
4
1.62 %
0
0.00 %
PAR-RECUR
1
42
0.01 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
17
1,995
0.68 %
58
2.91 %
1858
93.13 %
96
4.81 %
77
3.86 %
2
0.10 %
PAR-PERSIS
4
379
0.13 %
7
1.85 %
353
93.14 %
22
5.80 %
19
5.01 %
0
0.00 %
Cheshire
PAR-CUL-N
68
10,220
3.48 %
333
3.26 %
9424
92.21 %
615
6.02 %
462
4.52 %
1
0.01 %
PAR-NEW-IN
43
6,646
2.27 %
188
2.83 %
6115
92.01 %
424
6.38 %
335
5.04 %
8
0.12 %
PAR-PERSIS
4
348
0.12 %
12
3.45 %
316
90.80 %
22
6.32 %
19
5.46 %
1
0.29 %
PAR-RECUR
1
15
0.01 %
0
0.00 %
12
80.00 %
4
26.67 %
3
20.00 %
0
0.00 %
Derbyshire
PAR-CUL-N
7
985
0.34 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
132
14,085
4.80 %
578
4.10 %
12248
86.96 %
1412
10.02 %
1191
8.46 %
68
0.48 %
PAR-RECUR
2
309
0.11 %
8
2.59 %
247
79.94 %
63
20.39 %
54
17.48 %
0
0.00 %
E Sussex
PAR-NEW-IN
11
848
0.29 %
83
9.79 %
699
82.43 %
57
6.72 %
49
5.78 %
17
2.00 %
Hampshire
PAR-CUL-N
3
220
0.07 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
21
2,962
1.01 %
93
3.14 %
2636
88.99 %
247
8.34 %
231
7.80 %
2
0.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
1
76
0.03 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
40
4,599
1.57 %
201
4.37 %
4148
90.19 %
311
6.76 %
249
5.41 %
1
0.02 %
PAR-PERSIS
3
910
0.31 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
45
2,939
1.00 %
111
3.78 %
2652
90.23 %
240
8.17 %
175
5.95 %
1
0.03 %
PAR-PERSIS
3
184
0.06 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
10
1,219
0.42 %
46
3.77 %
1099
90.16 %
102
8.37 %
73
5.99 %
1
0.08 %
Oxon
PAR-CUL-P
2
111
0.04 %
6
5.41 %
88
79.28 %
17
15.32 %
17
15.32 %
0
0.00 %
PAR-NEW-IN
73
5,410
1.84 %
212
3.92 %
4834
89.35 %
433
8.00 %
360
6.65 %
4
0.07 %
PAR-PERSIS
16
3,050
1.04 %
130
4.26 %
2785
91.31 %
168
5.51 %
135
4.43 %
0
0.00 %
PAR-RECUR
3
452
0.15 %
17
3.76 %
405
89.60 %
34
7.52 %
30
6.64 %
0
0.00 %
Warks
PAR-NEW-IN
60
4,463
1.52 %
164
3.67 %
4017
90.01 %
314
7.04 %
272
6.09 %
10
0.22 %
PAR-PERSIS
3
163
0.06 %
10
6.13 %
141
86.50 %
13
7.98 %
12
7.36 %
0
0.00 %
PAR-RECUR
3
148
0.05 %
1
0.68 %
77
52.03 %
7
4.73 %
6
4.05 %
64
43.24 %
England - LRA
Beds
PAR-NEW-IN
7
926
0.32 %
34
3.67 %
817
88.23 %
93
10.04 %
69
7.45 %
6
0.65 %
Cleveland
PAR-NEW-IN
2
194
0.07 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
19
2,798
0.95 %
70
2.50 %
2463
88.03 %
293
10.47 %
254
9.08 %
11
0.39 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.03 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Essex
PAR-NEW-IN
3
247
0.08 %
14
5.67 %
203
82.19 %
19
7.69 %
14
5.67 %
16
6.48 %
Gr Manchester
PAR-NEW-IN
8
578
0.20 %
20
3.46 %
535
92.56 %
29
5.02 %
23
3.98 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.04 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Humberside
PAR-NEW-IN
1
118
0.04 %
4
3.39 %
109
92.37 %
6
5.08 %
5
4.24 %
0
0.00 %
Lancashire
PAR-NEW-IN
8
803
0.27 %
24
2.99 %
734
91.41 %
45
5.60 %
41
5.11 %
4
0.50 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Lincs
PAR-NEW-IN
22
1,822
0.62 %
57
3.13 %
1629
89.41 %
154
8.45 %
129
7.08 %
7
0.38 %
N Yorks
PAR-NEW-IN
15
532
0.18 %
27
5.08 %
390
73.31 %
123
23.12 %
113
21.24 %
2
0.38 %
Suffolk
PAR-NEW-IN
4
328
0.11 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.07 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England
2028
227569
77.57 %
11338
4.98 %
201333
88.47 %
17074
7.50 %
14147
6.22 %
751
0.33 %
Wales - High TB
Carms
PAR-IR-EX
9
48
0.02 %
21
43.75 %
24
50.00 %
2
4.17 %
2
4.17 %
1
2.08 %
PAR-IR-SEV
62
510
0.17 %
139
27.25 %
340
66.67 %
34
6.67 %
31
6.08 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
23
607
0.21 %
105
17.30 %
472
77.76 %
37
6.10 %
28
4.61 %
2
0.33 %
PAR-PERSIS
38
4,211
1.44 %
157
3.73 %
3889
92.35 %
219
5.20 %
161
3.82 %
4
0.09 %
PAR-RAPID
82
247
0.08 %
76
30.77 %
164
66.40 %
8
3.24 %
7
2.83 %
0
0.00 %
PAR-RECUR
8
581
0.20 %
27
4.65 %
530
91.22 %
33
5.68 %
22
3.79 %
2
0.34 %
SER-FLEXI
12
535
0.18 %
53
9.91 %
457
85.42 %
32
5.98 %
24
4.49 %
1
0.19 %
Ceredigion
PAR-IR-EX
10
31
0.01 %
7
22.58 %
24
77.42 %
1
3.23 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
49
340
0.12 %
82
24.12 %
241
70.88 %
21
6.18 %
15
4.41 %
2
0.59 %
PAR-OTHER
3
64
0.02 %
6
9.38 %
53
82.81 %
7
10.94 %
5
7.81 %
0
0.00 %
PAR-PERSIS
17
1,413
0.48 %
104
7.36 %
1225
86.69 %
96
6.79 %
71
5.02 %
13
0.92 %
PAR-RAPID
47
125
0.04 %
31
24.80 %
87
69.60 %
10
8.00 %
7
5.60 %
0
0.00 %
PAR-RECUR
2
7
0.00 %
1
14.29 %
4
57.14 %
3
42.86 %
2
28.57 %
0
0.00 %
SER-FLEXI
6
215
0.07 %
18
8.37 %
190
88.37 %
8
3.72 %
6
2.79 %
1
0.47 %
Gwent
PAR-IR-EX
3
29
0.01 %
11
37.93 %
18
62.07 %
2
6.90 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
10
83
0.03 %
11
13.25 %
66
79.52 %
8
9.64 %
6
7.23 %
0
0.00 %
PAR-OTHER
7
389
0.13 %
18
4.63 %
349
89.72 %
33
8.48 %
21
5.40 %
1
0.26 %
PAR-PERSIS
12
485
0.17 %
34
7.01 %
421
86.80 %
31
6.39 %
28
5.77 %
2
0.41 %
PAR-RAPID
9
27
0.01 %
6
22.22 %
21
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
116
0.04 %
10
8.62 %
100
86.21 %
6
5.17 %
6
5.17 %
0
0.00 %
SER-FLEXI
2
28
0.01 %
5
17.86 %
19
67.86 %
4
14.29 %
4
14.29 %
0
0.00 %
Pembs
PAR-HERD-S
4
19
0.01 %
2
10.53 %
12
63.16 %
6
31.58 %
5
26.32 %
0
0.00 %
PAR-IR-EX
40
208
0.07 %
62
29.81 %
136
65.38 %
12
5.77 %
9
4.33 %
1
0.48 %
PAR-IR-SEV
181
1,387
0.47 %
343
24.73 %
992
71.52 %
62
4.47 %
50
3.60 %
2
0.14 %
PAR-OTHER
35
1,310
0.45 %
82
6.26 %
1170
89.31 %
66
5.04 %
55
4.20 %
3
0.23 %
PAR-PERSIS
111
6,871
2.34 %
292
4.25 %
6261
91.12 %
407
5.92 %
308
4.48 %
10
0.15 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
194
516
0.18 %
158
30.62 %
330
63.95 %
35
6.78 %
28
5.43 %
0
0.00 %
PAR-RECUR
41
5,091
1.74 %
195
3.83 %
4697
92.26 %
239
4.69 %
184
3.61 %
15
0.29 %
SER-FLEXI
63
2,906
0.99 %
227
7.81 %
2551
87.78 %
154
5.30 %
116
3.99 %
12
0.41 %
Powys
PAR-HERD-S
1
10
0.00 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-IR-EX
16
99
0.03 %
26
26.26 %
69
69.70 %
6
6.06 %
4
4.04 %
0
0.00 %
PAR-IR-SEV
27
160
0.05 %
31
19.38 %
119
74.38 %
12
7.50 %
10
6.25 %
0
0.00 %
PAR-NEW-IN
1
239
0.08 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
19
1,163
0.40 %
51
4.39 %
1033
88.82 %
87
7.48 %
70
6.02 %
9
0.77 %
PAR-PERSIS
43
2,064
0.70 %
84
4.07 %
1754
84.98 %
237
11.48 %
216
10.47 %
10
0.48 %
PAR-RAPID
33
65
0.02 %
10
15.38 %
52
80.00 %
4
6.15 %
3
4.62 %
0
0.00 %
PAR-RECUR
16
1,810
0.62 %
65
3.59 %
xx-xxxx-xxxx
90.94 %
112
6.19 %
92
5.08 %
7
0.39 %
SER-FLEXI
13
249
0.08 %
24
9.64 %
194
77.91 %
35
14.06 %
31
12.45 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.35 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
3
25
0.01 %
7
28.00 %
18
72.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
5
140
0.05 %
5
3.57 %
122
87.14 %
14
10.00 %
12
8.57 %
1
0.71 %
PAR-PERSIS
2
136
0.05 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.00 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-IR-EX
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
16
1,392
0.47 %
114
8.19 %
1188
85.34 %
108
7.76 %
74
5.32 %
16
1.15 %
PAR-PERSIS
6
435
0.15 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
10
19
0.01 %
5
26.32 %
14
73.68 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.07 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.03 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
8
0.00 %
6
75.00 %
2
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
18
0.01 %
2
11.11 %
16
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
106
0.04 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
73
0.02 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
4
21
0.01 %
12
57.14 %
9
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.05 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.05 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.16 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
Mid Glamorgan
PAR-OTHER
1
8
0.00 %
3
37.50 %
5
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
4
10
0.00 %
4
40.00 %
6
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
48
349
0.12 %
53
15.19 %
286
81.95 %
13
3.72 %
10
2.87 %
0
0.00 %
PAR-NEW-IN
129
10,842
3.70 %
352
3.25 %
9848
90.83 %
771
7.11 %
629
5.80 %
13
0.12 %
PAR-OTHER
8
112
0.04 %
7
6.25 %
98
87.50 %
10
8.93 %
7
6.25 %
0
0.00 %
PAR-PERSIS
26
2,113
0.72 %
56
2.65 %
1955
92.52 %
139
6.58 %
102
4.83 %
0
0.00 %
PAR-POSTM
39
175
0.06 %
11
6.29 %
154
88.00 %
11
6.29 %
10
5.71 %
0
0.00 %
PAR-RAPID
31
96
0.03 %
21
21.88 %
71
73.96 %
5
5.21 %
4
4.17 %
0
0.00 %
PAR-RECUR
5
267
0.09 %
24
8.99 %
232
86.89 %
13
4.87 %
11
4.12 %
0
0.00 %
SER-FLEXI
23
830
0.28 %
49
5.90 %
752
90.60 %
42
5.06 %
29
3.49 %
0
0.00 %
Powys
PAR-IR-EX
2
5
0.00 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
20
1,884
0.64 %
53
2.81 %
xx-xxxx-xxxx
89.28 %
163
8.65 %
146
7.75 %
3
0.16 %
PAR-OTHER
3
126
0.04 %
0
0.00 %
123
97.62 %
5
3.97 %
3
2.38 %
0
0.00 %
PAR-PERSIS
3
44
0.01 %
5
11.36 %
32
72.73 %
8
18.18 %
7
15.91 %
0
0.00 %
PAR-POSTM
9
24
0.01 %
4
16.67 %
19
79.17 %
2
8.33 %
1
4.17 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
196
0.07 %
8
4.08 %
169
86.22 %
23
11.73 %
18
9.18 %
1
0.51 %
S Glamorgan
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
15
0.01 %
8
53.33 %
7
46.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
221
0.08 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-IR-SEV
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
126
0.04 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Low TB
Gwynedd
PAR-HERD-S
3
57
0.02 %
1
1.75 %
55
96.49 %
1
1.75 %
1
1.75 %
0
0.00 %
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
53
2,423
0.83 %
72
2.97 %
2178
89.89 %
218
9.00 %
168
6.93 %
5
0.21 %
PAR-OTHER
2
51
0.02 %
0
0.00 %
50
98.04 %
2
3.92 %
1
1.96 %
0
0.00 %
PAR-POSTM
19
60
0.02 %
1
1.67 %
55
91.67 %
5
8.33 %
4
6.67 %
0
0.00 %
PAR-RAPID
4
5
0.00 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
65
0.02 %
7
10.77 %
53
81.54 %
6
9.23 %
5
7.69 %
0
0.00 %
NE Wales
PAR-HERD-S
4
111
0.04 %
9
8.11 %
99
89.19 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-IR-EX
2
7
0.00 %
1
14.29 %
5
71.43 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-NEW-IN
50
3,555
1.21 %
169
4.75 %
3102
87.26 %
327
9.20 %
277
7.79 %
7
0.20 %
PAR-OTHER
4
152
0.05 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.01 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
PAR-POSTM
40
83
0.03 %
4
4.82 %
77
92.77 %
6
7.23 %
2
2.41 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
11
962
0.33 %
60
6.24 %
851
88.46 %
58
6.03 %
51
5.30 %
0
0.00 %
Wales
1902
63779
21.74 %
3919
6.14 %
56304
88.28 %
4253
6.67 %
3396
5.32 %
160
0.25 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.06 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
9
892
0.30 %
114
12.78 %
709
79.48 %
66
7.40 %
59
6.61 %
10
1.12 %
PAR-PERSIS
1
108
0.04 %
7
6.48 %
97
89.81 %
7
6.48 %
4
3.70 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.00 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Wigtown
PAR-NEW-IN
6
811
0.28 %
13
1.60 %
734
90.51 %
74
9.12 %
63
7.77 %
1
0.12 %
Scotland
22
2023
0.69 %
150
7.41 %
1724
85.22 %
161
7.96 %
137
6.77 %
12
0.59 %
Grand Total
Sum:
3952 293371
15407
259361
21488
17680
923
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol December 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
102
10434
99.9%
526
5.0%
9230
88.5%
752
7.2%
656
6.3%
22
0.2%
Serial/Extended
2
7
0.1%
1
14.3%
5
71.4%
1
14.3%
1
14.3%
0
0.0 %
Total
104
10441
100.0%
527
5.0%
9235
88.4%
753
7.2%
657
6.3%
22
0.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
126
1787
66.8%
154
8.6%
1489
83.3%
167
9.3%
141
7.9%
3
0.2%
Serial/Extended
8
889
33.2%
78
8.8%
753
84.7%
59
6.6%
54
6.1%
4
0.4%
Total
134
2676
100.0%
232
8.7%
2242
83.8%
226
8.4%
195
7.3%
7
0.3%
GB
Parallel
228
12221
93.2%
680
5.6%
10719
87.7%
919
7.5%
797
6.5%
25
0.2%
Serial/Extended
10
896
6.8%
79
8.8%
758
84.6%
60
6.7%
55
6.1%
4
0.4%
Total
238
13117
100.0%
759
5.8%
11477
87.5%
979
7.5%
852
6.5%
29
0.2%
104.00
134.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
2006 226479
99.5% 11263
5.0% 200370
88.5%
17026
7.5%
14108
6.2%
738
0.3%
Serial/Extended
22
1090
0.5%
75
6.9%
963
88.3%
48
4.4%
39
3.6%
13
1.2%
Total
xx-xxxx-xxxx
100.0% 11338
5.0% 201333
88.5%
17074
7.5%
14147
6.2%
751
0.3%
Scotland
Parallel
21
2019
99.8%
150
7.4%
1721
85.2%
161
8.0%
137
6.8%
11
0.5%
Serial/Extended
1
4
0.2%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
22
2023
100.0%
150
7.4%
1724
85.2%
161
8.0%
137
6.8%
12
0.6%
Wales
Parallel
1761
57756
90.6%
3448
6.0%
51038
88.4%
3905
6.8%
3125
5.4%
145
0.3%
Serial/Extended
141
6023
9.4%
471
7.8%
5266
87.4%
348
5.8%
271
4.5%
15
0.2%
Total
1902
63779
100.0%
3919
6.1%
56304
88.3%
4253
6.7%
3396
5.3%
160
0.3%
GB
Parallel
xx-xxxx-xxxx
97.6% 14861
5.2% 253129
88.4%
21092
7.4%
17370
6.1%
894
0.3%
Serial/Extended
164
7117
2.4%
546
7.7%
6232
87.6%
396
5.6%
310
4.4%
29
0.4%
Total
3952 293371
100.0% 15407
5.3% 259361
88.4%
21488
7.3%
17680
6.0%
923
0.3%
#######
#######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
892
6.8%
79
8.9%
755
84.6%
59
6.6 %
54
6.1 %
4
0.4 %
Parallel - IRs in Exit Strategy for persistent her
9
24
0.2%
6
25.0%
18
75.0%
2
8.3 %
0
0
Parallel New Infection
24
1948
14.9%
79
4.1%
xx-xxxx-xxxx
86.8%
179
9.2 %
174
8.9 %
5
0.3 %
Parallel - new infection cull area
15
987
7.5%
63
6.4%
848
85.9%
88
8.9 %
76
7.7 %
0
Parallel - Other
17
849
6.5%
42
4.9%
754
88.8%
65
7.7 %
52
6.1 %
1
0.1 %
Parallel Persistent Infection
23
2480
18.9%
105
4.2%
2186
88.1%
224
9.0 %
186
7.5 %
3
0.1 %
Parallel - persistent infection cull area
3
90
0.7%
5
5.6%
69
76.7%
18
20.0 %
16
17.8 %
0
Parallel – post-mvt test coupled with IDEXX an
11
61
0.5%
5
8.2%
52
85.2%
4
6.6 %
4
6.6 %
0
Parallel - Rapid Testing Two-times IRs
34
65
0.5%
15
23.1%
46
70.8%
5
7.7 %
4
6.2 %
0
Parallel - Recurrent breakdowns
54
5443
41.5%
297
5.5%
4856
89.2%
320
5.9 %
275
5.1 %
15
0.3 %
Parallel - severe IRs - in persistent herds
38
274
2.1%
63
23.0%
200
73.0%
14
5.1 %
10
3.6 %
1
0.4 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
1
25.0 %
1
25.0 %
0
Total
238
13117
100.0%
759
5.8%
11477
87.5%
979
7.5 %
852
6.5 %
29
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (December 2021)
England - HRA
Extended Flexible IFN-GAMMA test
1
3
0.0%
1
33.3%
2
66.7%
0
0
0
Parallel New Infection
6
549
4.2%
22
4.0%
482
87.8%
43
7.8 %
41
7.5 %
4
0.7 %
Parallel - new infection cull area
15
987
7.5%
63
6.4%
848
85.9%
88
8.9 %
76
7.7 %
0
Parallel - Other
7
494
3.8%
21
4.3%
452
91.5%
28
5.7 %
20
4.0 %
1
0.2 %
Parallel Persistent Infection
14
2123
16.2%
95
4.5%
1853
87.3%
203
9.6 %
172
8.1 %
3
0.1 %
Parallel - persistent infection cull area
3
90
0.7%
5
5.6%
69
76.7%
18
20.0 %
16
17.8 %
0
Parallel - Recurrent breakdowns
44
5112
39.0%
285
5.6%
4564
89.3%
292
5.7 %
249
4.9 %
14
0.3 %
England - HRA
90
9358
71.3%
492
5.3%
8270
88.4%
672
7.2 %
574
6.1 %
22
0.2 %
England - Edge
Parallel New Infection
9
745
5.7%
25
3.4%
651
87.4%
60
8.1 %
69
9.3 %
0
Parallel Persistent Infection
2
318
2.4%
10
3.1%
299
94.0%
15
4.7 %
9
2.8 %
0
Parallel - Recurrent breakdowns
1
15
0.1%
0
0.0 %
12
80.0%
4
26.7 %
3
20.0 %
0
England - Edge
12
1078
8.2%
35
3.2%
962
89.2%
79
7.3 %
81
7.5 %
0
England - LRA
Parallel New Infection
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
1
25.0 %
1
25.0 %
0
England - LRA
2
5
0.0%
0
0.0 %
3
60.0%
2
40.0 %
2
40.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
7
880
6.7%
77
8.8%
747
84.9%
57
6.5 %
52
5.9 %
4
0.5 %
Parallel - IRs in Exit Strategy for persistent her
7
20
0.2%
6
30.0%
14
70.0%
2
10.0 %
0
0
Parallel - Other
3
226
1.7%
11
4.9%
194
85.8%
22
9.7 %
21
9.3 %
0
Parallel Persistent Infection
6
37
0.3%
0
0.0 %
33
89.2%
5
13.5 %
4
10.8 %
0
Parallel - Rapid Testing Two-times IRs
24
38
0.3%
7
18.4%
27
71.1%
5
13.2 %
4
10.5 %
0
Parallel - Recurrent breakdowns
9
316
2.4%
12
3.8%
280
88.6%
24
7.6 %
23
7.3 %
1
0.3 %
Parallel - severe IRs - in persistent herds
27
167
1.3%
48
28.7%
108
64.7%
13
7.8 %
10
6.0 %
1
0.6 %
Wales - High TB
83
xx-xxxx-xxxx
12.8%
161
9.6%
1403
83.3%
128
7.6 %
114
6.8 %
6
0.4 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
2
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Parallel New Infection
4
331
2.5%
13
3.9%
290
87.6%
33
10.0 %
28
8.5 %
0
Parallel - Other
7
129
1.0%
10
7.8%
108
83.7%
15
11.6 %
11
8.5 %
0
Parallel Persistent Infection
1
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel – post-mvt test coupled with IDEXX an
6
32
0.2%
3
9.4%
26
81.3%
3
9.4 %
3
9.4 %
0
Parallel - Rapid Testing Two-times IRs
10
27
0.2%
8
29.6%
19
70.4%
0
0
0
Parallel - severe IRs - in persistent herds
11
107
0.8%
15
14.0%
92
86.0%
1
0.9 %
0
0
Wales - Intermediate TB
41
632
4.8%
49
7.8%
540
85.4%
53
8.4 %
43
6.8 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
9
0.1%
1
11.1%
6
66.7%
2
22.2 %
2
22.2 %
0
Parallel New Infection
4
322
2.5%
19
5.9%
267
82.9%
42
13.0 %
35
10.9 %
1
0.3 %
Parallel – post-mvt test coupled with IDEXX an
5
29
0.2%
2
6.9%
26
89.7%
1
3.4 %
1
3.4 %
0
Wales - Low TB
10
360
2.7%
22
6.1%
299
83.1%
45
12.5 %
38
10.6 %
1
0.3 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
157
7024
2.4%
546
7.8%
6147
87.5%
388
5.5 %
304
4.3 %
27
0.4 %
Parallel - Herd or Group Slaughter
22
1633
0.6%
98
6.0%
1465
89.7%
78
4.8 %
65
4.0 %
5
0.3 %
Parallel - IRs in Exit Strategy for persistent herds
89
449
0.2%
139
31.0%
292
65.0%
24
5.3 %
16
3.6 %
2
0.4 %
Parallel New Infection
876
79242
27.0%
3088
3.9%
70478
88.9%
6509
8.2 %
5457
6.9 %
219
0.3 %
Parallel - new infection cull area
984 110595
37.7%
5905
5.3%
97913
88.5%
7842
7.1 %
6423
5.8 %
354
0.3 %
Parallel - Other
176
10097
3.4%
696
6.9%
8759
86.7%
704
7.0 %
574
5.7 %
68
0.7 %
Parallel Persistent Infection
399
39239
13.4%
1878
4.8%
34780
88.6%
3082
7.9 %
2510
6.4 %
71
0.2 %
Parallel - persistent infection cull area
92
12040
4.1%
742
6.2%
10589
87.9%
821
6.8 %
653
5.4 %
56
0.5 %
Parallel – post-mvt test coupled with IDEXX anti
107
342
0.1%
20
5.8%
305
89.2%
24
7.0 %
17
5.0 %
0
Parallel - Rapid Testing Two-times IRs
423
1146
0.4%
330
28.8%
767
66.9%
63
5.5 %
49
4.3 %
0
Parallel - Recurrent breakdowns
236
28586
9.7%
1296
4.5%
25691
89.9%
1795
6.3 %
1484
5.2 %
115
0.4 %
Parallel - severe IRs - in persistent herds
384
2885
1.0%
669
23.2%
2090
72.4%
150
5.2 %
122
4.2 %
4
0.1 %
Serial - Other
2
59
0.0%
0
0.0 %
57
96.6%
4
6.8 %
2
3.4 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
6
75.0%
1
12.5 %
1
12.5 %
1
12.5 %
Total
3952 293371
100.0%
15407
5.3%
259361
88.4%
21488
7.3 %
17680
6.0 %
923
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
16
1001
0.3%
75
7.5%
881
88.0%
40
4.0 %
33
3.3 %
12
1.2 %
Parallel - Herd or Group Slaughter
3
397
0.1%
38
9.6%
344
86.6%
19
4.8 %
15
3.8 %
0
Parallel New Infection
27
2390
0.8%
166
6.9%
2041
85.4%
186
7.8 %
176
7.4 %
7
0.3 %
Parallel - new infection cull area
904
99135
33.8%
5503
5.6%
87451
88.2%
7091
7.2 %
5830
5.9 %
351
0.4 %
Parallel - Other
46
4476
1.5%
295
6.6%
3862
86.3%
317
7.1 %
285
6.4 %
34
0.8 %
Parallel Persistent Infection
98
15886
5.4%
896
5.6%
13629
85.8%
1596
10.0 %
1332
8.4 %
29
0.2 %
Parallel - persistent infection cull area
88
11610
4.0%
733
6.3%
10209
87.9%
794
6.8 %
630
5.4 %
38
0.3 %
Parallel - Recurrent breakdowns
139
18973
6.5%
890
4.7%
17031
89.8%
1223
6.4 %
1027
5.4 %
25
0.1 %
Serial - Other
2
59
0.0%
0
0.0 %
57
96.6%
4
6.8 %
2
3.4 %
0
England - Edge
Parallel New Infection
472
46646
15.9%
1817
3.9%
41602
89.2%
3748
8.0 %
3113
6.7 %
114
0.2 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - new infection cull area
79
11442
3.9%
402
3.5%
10456
91.4%
741
6.5 %
583
5.1 %
1
0.0 %
Parallel Persistent Infection
38
5357
1.8%
199
3.7%
4902
91.5%
307
5.7 %
254
4.7 %
2
0.0 %
Parallel - persistent infection cull area
4
430
0.1%
9
2.1%
380
88.4%
27
6.3 %
23
5.3 %
18
4.2 %
Parallel - Recurrent breakdowns
10
966
0.3%
27
2.8%
779
80.6%
111
11.5 %
96
9.9 %
64
6.6 %
England - LRA
Parallel New Infection
97
8753
3.0%
288
3.3%
7678
87.7%
856
9.8 %
734
8.4 %
53
0.6 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
1
25.0 %
1
25.0 %
0
England
xx-xxxx-xxxx
77.6%
11338
5.0%
201333
88.5%
17074
7.5 %
14147
6.2 %
751
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
96
3933
1.3%
327
8.3%
3411
86.7%
233
5.9 %
181
4.6 %
14
0.4 %
Parallel - Herd or Group Slaughter
10
1059
0.4%
49
4.6%
960
90.7%
52
4.9 %
45
4.2 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
78
415
0.1%
127
30.6%
271
65.3%
23
5.5 %
15
3.6 %
2
0.5 %
Parallel New Infection
2
245
0.1%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
92
3673
1.3%
267
7.3%
3199
87.1%
244
6.6 %
191
5.2 %
16
0.4 %
Parallel Persistent Infection
223
15180
5.2%
673
4.4%
13679
90.1%
995
6.6 %
789
5.2 %
39
0.3 %
Parallel - Rapid Testing Two-times IRs
368
988
0.3%
282
28.5%
661
66.9%
58
5.9 %
45
4.6 %
0
Parallel - Recurrent breakdowns
70
7605
2.6%
298
3.9%
6977
91.7%
393
5.2 %
306
4.0 %
24
0.3 %
Parallel - severe IRs - in persistent herds
332
2505
0.9%
613
24.5%
1776
70.9%
137
5.5 %
112
4.5 %
4
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
30
1063
0.4%
77
7.2%
951
89.5%
51
4.8 %
34
3.2 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
8
26
0.0%
10
38.5%
16
61.5%
0
0
0
Parallel New Infection
155
13319
4.5%
427
3.2%
12035
90.4%
1000
7.5 %
836
6.3 %
21
0.2 %
Parallel - Other
32
1745
0.6%
130
7.4%
1508
86.4%
133
7.6 %
90
5.2 %
17
1.0 %
Parallel Persistent Infection
37
2665
0.9%
101
3.8%
2433
91.3%
177
6.6 %
131
4.9 %
0
Parallel – post-mvt test coupled with IDEXX ant
48
199
0.1%
15
7.5%
173
86.9%
13
6.5 %
11
5.5 %
0
Parallel - Rapid Testing Two-times IRs
50
152
0.1%
46
30.3%
102
67.1%
5
3.3 %
4
2.6 %
0
Parallel - Recurrent breakdowns
17
1042
0.4%
81
7.8%
904
86.8%
68
6.5 %
55
5.3 %
2
0.2 %
Parallel - severe IRs - in persistent herds
51
379
0.1%
56
14.8%
313
82.6%
13
3.4 %
10
2.6 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
15
1027
0.4%
67
6.5%
904
88.0%
64
6.2 %
56
5.5 %
0
Parallel - Herd or Group Slaughter
7
168
0.1%
10
6.0%
154
91.7%
6
3.6 %
4
2.4 %
0
Parallel - IRs in Exit Strategy for persistent herd
3
8
0.0%
2
25.0%
5
62.5%
1
12.5 %
1
12.5 %
0
Parallel New Infection
103
5978
2.0%
241
4.0%
5280
88.3%
545
9.1 %
445
7.4 %
12
0.2 %
Parallel - Other
6
203
0.1%
4
2.0%
190
93.6%
10
4.9 %
8
3.9 %
1
0.5 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel – post-mvt test coupled with IDEXX ant
59
143
0.0%
5
3.5%
132
92.3%
11
7.7 %
6
4.2 %
0
Parallel - Rapid Testing Two-times IRs
5
6
0.0%
2
33.3%
4
66.7%
0
0
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
1902
63779
21.7%
3919
6.1%
56304
88.3%
4253
6.7 %
3396
5.3 %
160
0.3 %
Scotland - Scotland
Parallel New Infection
20
1911
0.7%
143
7.5%
1624
85.0%
154
8.1 %
133
7.0 %
11
0.6 %
Parallel Persistent Infection
1
108
0.0%
7
6.5%
97
89.8%
7
6.5 %
4
3.7 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
22
2023
0.7%
150
7.4%
1724
85.2%
161
8.0 %
137
6.8 %
12
0.6 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
0
84
5
12
March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
4
0
9
0
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
4
0
4
0
1083
0
92
0
6
July
298
24841
164
1077
30
0
0
0
0
61
4
0
4
0
936
0
40
0
2
August
312
17920
167
912
3
26
0
0
0
0
35
0
0
7
2
755
2
80
1
1
September
274
15805
130
890
13
0
0
0
0
95
0
0
7
1
743
1
27
0
2
October
276
19790
140
814
20
0
0
0
0
45
0
12
8
1
692
33
1
1
1
November
329
25737
195
1349
340
0
0
0
0
68
0
19
0
0
874
48
0
0
0
December
238
13117
138
759
386
113
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Totals (last
12 months)
3952
293371
2291
15407
395
780
0
0
0
0
930
28
31
86
4
12049
85
720
10
25
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2028
227569
1331
11338
584
0
0
0
0
805
12
17
44
3
9303
10
84
0
2
Scotland
22
2023
15
150
0
0
0
0
0
47
1
0
6
0
53
2
21
3
17
Wales
1902
63779
945
3919
196
0
0
0
0
78
15
14
36
1
2693
73
615
7
6
Totals
3952
293371
2291
15407
780
0
0
0
0
930
28
31
86
4
12049
85
720
10
25
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2020
2021
IFNg Positive herds
IFNg Negative herds
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
50
100
150
200
250
Wiltshire
Staffs
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
NE Wales
Lincs
Lancashire
Heref
Hampshire
Gwynedd
Gwent
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during December 2021 (% submission per
County)
County
%Resampled %Retested
Avon
8.00 %
6.15 %
Bucks
Carms
6.62 %
8.61 %
Ceredigion
11.11 %
11.11 %
Cheshire
5.57 %
5.28 %
Corn & Scilly
5.59 %
6.81 %
Derbyshire
12.50 %
13.75 %
Devon
5.99 %
7.39 %
Dorset
6.04 %
7.17 %
E Sussex
4.76 %
7.14 %
Gloucs
6.69 %
9.24 %
Gwent
50.00 %
100.00 %
Gwynedd
6.76 %
9.46 %
Hampshire
38.60 %
17.54 %
Heref
3.88 %
5.26 %
Lancashire
25.00 %
25.00 %
Lincs
100.00 %
100.00 %
NE Wales
8.17 %
9.58 %
Oxon
2.91 %
4.65 %
Pembs
5.77 %
6.35 %
Powys
11.00 %
11.68 %
S Glamorgan
Shropshire
8.73 %
9.32 %
Somerset excl
6.01 %
6.35 %
Staffs
5.86 %
7.51 %
Wiltshire
6.62 %
6.84 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
10307
527
5.1%
10441
527
5.0%
Wales
2633
232
8.8%
2676
232
8.7%
GB
12940
759
5.9%
13117
759
5.8%
Table 6a (i): Number of animals and submissions tested in December 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
210457
11335
5.4%
227569
11338
5.0%
Wales
59047
3918
6.6%
63779
3919
6.1%
Scotland
1386
150
10.8%
2023
150
7.4%
GB
270879
15403
5.7%
293371
15407
5.3%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19680
1005
5.1%
19851
1005
5.1%
Wales
3721
193
5.2%
3825
193
5.0%
Scotland
453
30
6.6%
470
30
6.4%
GB
23853
1228
5.1%
24146
1228
5.1%
Table 6a (ii): Number of animals and submissions tested in December 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
209635
8856
4.2%
221766
8856
4.0%
Wales
51713
3116
6.0%
56402
3117
5.5%
Scotland
5354
161
3.0%
6279
161
2.6%
GB
266699
12133
4.5%
284447
12134
4.3%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Wiltshire
PRV-SE
2
20
0
0
Total
2
20
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples December 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
2
2
0
0
Derbyshire
PRV-SP
2
2
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
2
3
0
0
Dorset
PRV-SE
1
18
5
27.78 %
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
7
382
18
4.71 %
PRV-SP
4
6
0
0
Staffordshire
PRV-SP
2
2
0
0
Wiltshire
PRV-SE
3
30
0
0
PRV-SP
1
3
0
0
Worcestershire
PRV-SE
1
1
0
0
PRV-SP
4
6
0
0
Total
37
535
27
5.05 %
Isle of Man
SER-IOM
7
8
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
7
44
14
31.8%
Ceredigion
BOV-IR-C
4
26
3
11.5%
BOV-IR-EX
1
2
0
0
North East Wales
BOV-IR-C
11
118
10
8.5%
BOV-POSTM
9
21
9
42.9%
North West Wales
BOV-IR-EX
1
1
0
0
BOV-POSTM
1
20
3
15.0%
Pembrokeshire
BOV-IR-C
18
113
27
23.9%
BOV-IR-EX
5
20
5
25.0%
BOV-OTHER
3
393
12
3.1%
BOV-P
4
180
11
6.1%
Powys
BOV-IR-C
4
34
7
20.6%
BOV-IR-EX
1
7
2
28.6%
South Wales
BOV-IR-EX
1
13
5
38.5%
Wales
70
992
108
10.9%
All GB
70
992
108
10.9 %
Table 8a Number of Cattle Antibody Tests December 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
3
594
21
3.5%
Devon
1
95
6
6.3%
BOV-P
2
47
0
0
Staffordshire
BOV-P
1
30
1
3.3%
Wiltshire
BOV-P
1
189
8
4.2%
England
8
955
36
3.8%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
50
533
129
24.2%
BOV-IR-EX
6
45
13
28.9%
BOV-OTHER
3
80
4
5.0%
BOV-P
2
87
3
3.4%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
43
417
68
16.3%
BOV-IR-EX
6
34
8
23.5%
BOV-OTHER
6
240
16
6.7%
BOV-P
2
79
2
2.5%
North East Wales
2
24
6
25.0%
BOV-IR-C
40
391
54
13.8%
BOV-IR-EX
2
5
1
20.0%
BOV-OTHER
5
150
5
3.3%
BOV-P
16
1,550
63
4.1%
BOV-POSTM
66
187
44
23.5%
OTHER
1
48
4
8.3%
North West Wales
BOV-IR-EX
1
1
0
0

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-P
1
42
0
0
BOV-POSTM
5
34
7
20.6%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
155
1,595
287
18.0%
BOV-IR-EX
25
185
38
20.5%
BOV-OTHER
21
1,248
60
4.8%
BOV-P
20
1,141
56
4.9%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
31
222
43
19.4%
BOV-IR-EX
5
62
14
22.6%
BOV-OTHER
5
227
30
13.2%
BOV-P
4
77
4
5.2%
BOV-POSTM
4
8
1
12.5%
South Wales
4
14
5
35.7%
BOV-IR-C
12
112
23
20.5%
BOV-IR-EX
2
34
6
17.6%
BOV-P
2
87
1
1.1%
Wales
577
9,299
1039
11.2%
All GB
585
10,254
1,075
10.5 %