Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - May 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 177
Report Period 1st - 31st May 2021
Issued 16 June 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
Parallel blood testing of skin test-negative cattle
in breakdowns herds occurring within 6 months
of lifting restrictions after a previous breakdown
NO (APHA discretion -
Wales only)
PAR-POSTM
Post movement blood test of cattle (coupled
with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Test Criteria

Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
May 2021
%
Total 2021
%
Submissions
357
1870
Samples
Total (%)
27182
100.00 %
148333
100.00 %
England
20660
76.01 %
114111
76.93 %
Wales
6394
23.52 %
33355
22.49 %
Scotland
128
0.47 %
867
0.58 %
Parallel Tests
PAR-CUL-N
11922
43.86 %
67476
45.49 %
PAR-CUL-P
1501
5.52 %
5075
3.42 %
PAR-HERD-S
48
0.18 %
1088
0.73 %
PAR-IR-EX
29
0.11 %
146
0.10 %
PAR-IR-SEV
338
1.24 %
914
0.62 %
PAR-NEW-IN
7153
26.32 %
44785
30.19 %
PAR-OTHER
1080
3.97 %
5351
3.61 %
PAR-PERSIS
2918
10.74 %
14893
10.04 %
PAR-RAPID
59
0.22 %
480
0.32 %
PAR-RECUR
913
3.36 %
4908
3.31 %
Total(% of all samples)
25961
95.51 %
145116
97.83 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
12
0.01 %
SER-FLEXI
1221
4.49 %
3149
2.12 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
52
0.04 %
Total(% of all samples)
1221
4.49 %
3217
2.17 %
Total
27182
100.00 %
148333
100.00 %
Retests
Total (% of all samples)
xx-xxxx-xxxx
6.21 %
9988
6.73 %
England
1326
4.88 %
7886
5.32 %
Wales
354
1.30 %
2035
1.37 %
Scotland
7
0.03 %
67
0.05 %
Resamples
Total (% of all samples)
1389
5.11 %
8587
5.79 %
England
1105
4.07 %
6874
4.63 %
Wales
281
1.03 %
1653
1.11 %
Scotland
3
0.01 %
60
0.04 %
Rejects
Total (% of all samples)
74
0.27 %
529
0.36 %
England
59
0.22 %
418
0.28 %
Wales
15
0.06 %
101
0.07 %
Scotland
0
0.00 %
10
0.01 %
Table 1. Monthly Totals for May 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
11922
43.9 %
PAR-CUL-P
1501
5.5 %
PAR-HERD-S
48
0.2 %
PAR-IR-EX
29
0.1 %
PAR-IR-SEV
338
1.2 %
PAR-NEW-IN
7153
26.3 %
PAR-OTHER
1080
4.0 %
PAR-PERSIS
2918
10.7 %
PAR-RAPID
59
0.2 %
PAR-RECUR
913
3.4 %
SER-FLEXI
1221
4.5 %
Total
27182
100.00 %
Figure 1. Number of samples submitted in each category during May 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Ayrshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Leics & Rut
Heref
Hampshire
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during May 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
1
30
0.11 %
3
10.00 %
22
73.33 %
5
16.67 %
5
16.67 %
0
0.00 %
PAR-PERSIS
1
61
0.22 %
1
1.64 %
55
90.16 %
5
8.20 %
5
8.20 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
15
1,382
5.08 %
56
4.05 %
1246
90.16 %
95
6.87 %
80
5.79 %
0
0.00 %
PAR-CUL-P
5
1,395
5.13 %
147
10.54 %
1171
83.94 %
81
5.81 %
77
5.52 %
0
0.00 %
SER-FLEXI
2
264
0.97 %
10
3.79 %
250
94.70 %
6
2.27 %
4
1.52 %
0
0.00 %
Devon
PAR-CUL-N
40
4,969
18.28 %
307
6.18 %
4429
89.13 %
255
5.13 %
198
3.98 %
35
0.70 %
PAR-CUL-P
1
50
0.18 %
4
8.00 %
32
64.00 %
14
28.00 %
14
28.00 %
0
0.00 %
PAR-NEW-IN
3
397
1.46 %
36
9.07 %
298
75.06 %
64
16.12 %
63
15.87 %
0
0.00 %
PAR-OTHER
2
23
0.08 %
2
8.70 %
17
73.91 %
4
17.39 %
4
17.39 %
0
0.00 %
Dorset
PAR-CUL-N
11
1,042
3.83 %
69
6.62 %
941
90.31 %
44
4.22 %
32
3.07 %
0
0.00 %
Gloucs
PAR-CUL-N
10
783
2.88 %
28
3.58 %
696
88.89 %
73
9.32 %
56
7.15 %
3
0.38 %
PAR-CUL-P
1
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Heref
PAR-OTHER
1
562
2.07 %
19
3.38 %
482
85.77 %
63
11.21 %
61
10.85 %
0
0.00 %
Shropshire
PAR-CUL-N
1
12
0.04 %
2
16.67 %
8
66.67 %
3
25.00 %
2
16.67 %
0
0.00 %
Somerset excl N
PAR-CUL-N
14
975
3.59 %
49
5.03 %
879
90.15 %
61
6.26 %
47
4.82 %
0
0.00 %
PAR-PERSIS
1
297
1.09 %
13
4.38 %
270
90.91 %
21
7.07 %
13
4.38 %
1
0.34 %
Staffs
PAR-CUL-N
10
1,208
4.44 %
67
5.55 %
1064
88.08 %
86
7.12 %
77
6.37 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
0
0.00 %
Wiltshire
PAR-CUL-N
2
69
0.25 %
2
2.90 %
64
92.75 %
2
2.90 %
2
2.90 %
1
1.45 %
PAR-CUL-P
2
21
0.08 %
2
9.52 %
12
57.14 %
6
28.57 %
6
28.57 %
1
4.76 %
PAR-PERSIS
2
428
1.57 %
23
5.37 %
391
91.36 %
11
2.57 %
7
1.64 %
7
1.64 %
SER-FLEXI
2
172
0.63 %
13
7.56 %
148
86.05 %
1
0.58 %
1
0.58 %
10
5.81 %
England - Edge
Berks
PAR-NEW-IN
4
539
1.98 %
31
5.75 %
463
85.90 %
48
8.91 %
45
8.35 %
0
0.00 %
PAR-PERSIS
1
25
0.09 %
0
0.00 %
23
92.00 %
3
12.00 %
2
8.00 %
0
0.00 %
Bucks
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-CUL-N
10
1,479
5.44 %
21
1.42 %
1386
93.71 %
85
5.75 %
72
4.87 %
0
0.00 %
Table 2a. Summary by County for May 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
6
604
2.22 %
17
2.81 %
554
91.72 %
35
5.79 %
33
5.46 %
0
0.00 %
PAR-PERSIS
1
273
1.00 %
5
1.83 %
259
94.87 %
12
4.40 %
9
3.30 %
0
0.00 %
Derbyshire
PAR-NEW-IN
13
1,207
4.44 %
55
4.56 %
1081
89.56 %
105
8.70 %
71
5.88 %
0
0.00 %
Hampshire
PAR-CUL-N
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Leics & Rut
PAR-PERSIS
3
910
3.35 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
3
74
0.27 %
4
5.41 %
67
90.54 %
5
6.76 %
3
4.05 %
0
0.00 %
PAR-PERSIS
1
13
0.05 %
2
15.38 %
9
69.23 %
3
23.08 %
2
15.38 %
0
0.00 %
Oxon
PAR-NEW-IN
8
1,204
4.43 %
38
3.16 %
1116
92.69 %
58
4.82 %
50
4.15 %
0
0.00 %
Warks
PAR-NEW-IN
5
182
0.67 %
8
4.40 %
161
88.46 %
14
7.69 %
13
7.14 %
0
0.00 %
England
185
20660
76.01 %
1058
5.12 %
18438
89.24 %
1326
6.42 %
1105
5.35 %
59
0.29 %
Wales - High TB
Carms
PAR-IR-EX
1
11
0.04 %
8
72.73 %
3
27.27 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
39
0.14 %
6
15.38 %
32
82.05 %
1
2.56 %
1
2.56 %
0
0.00 %
PAR-OTHER
3
45
0.17 %
8
17.78 %
36
80.00 %
1
2.22 %
1
2.22 %
0
0.00 %
PAR-PERSIS
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
8
0.03 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
30
0.11 %
10
33.33 %
20
66.67 %
1
3.33 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-IR-EX
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
8
63
0.23 %
14
22.22 %
46
73.02 %
5
7.94 %
3
4.76 %
0
0.00 %
PAR-RAPID
3
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Gwent
PAR-IR-SEV
1
7
0.03 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-PERSIS
1
9
0.03 %
0
0.00 %
4
44.44 %
5
55.56 %
5
55.56 %
0
0.00 %
PAR-RECUR
2
115
0.42 %
10
8.70 %
100
86.96 %
5
4.35 %
5
4.35 %
0
0.00 %
Pembs
PAR-IR-EX
4
8
0.03 %
3
37.50 %
2
25.00 %
3
37.50 %
3
37.50 %
0
0.00 %
PAR-IR-SEV
22
170
0.63 %
55
32.35 %
110
64.71 %
7
4.12 %
5
2.94 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
8
294
1.08 %
14
4.76 %
264
89.80 %
23
7.82 %
15
5.10 %
1
0.34 %
PAR-RAPID
18
38
0.14 %
15
39.47 %
21
55.26 %
4
10.53 %
2
5.26 %
0
0.00 %
PAR-RECUR
3
426
1.57 %
28
6.57 %
391
91.78 %
13
3.05 %
7
1.64 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
10
578
2.13 %
67
11.59 %
491
84.95 %
25
4.33 %
17
2.94 %
3
0.52 %
Powys
PAR-IR-EX
2
8
0.03 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
16
0.06 %
5
31.25 %
11
68.75 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
4
27
0.10 %
1
3.70 %
15
55.56 %
12
44.44 %
11
40.74 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
4
442
1.63 %
54
12.22 %
368
83.26 %
23
5.20 %
18
4.07 %
2
0.45 %
PAR-PERSIS
1
13
0.05 %
3
23.08 %
10
76.92 %
1
7.69 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
4
0.01 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.51 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
NE Wales
PAR-IR-SEV
8
43
0.16 %
6
13.95 %
34
79.07 %
3
6.98 %
3
6.98 %
0
0.00 %
PAR-NEW-IN
18
1,921
7.07 %
82
4.27 %
1760
91.62 %
92
4.79 %
76
3.96 %
3
0.16 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
565
2.08 %
18
3.19 %
522
92.39 %
32
5.66 %
25
4.42 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
12
0.04 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
SER-FLEXI
2
75
0.28 %
5
6.67 %
70
93.33 %
2
2.67 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
3
356
1.31 %
12
3.37 %
321
90.17 %
23
6.46 %
21
5.90 %
2
0.56 %
S Glamorgan
PAR-RECUR
2
221
0.81 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
5
227
0.84 %
10
4.41 %
200
88.11 %
18
7.93 %
14
6.17 %
3
1.32 %
NE Wales
PAR-HERD-S
2
48
0.18 %
2
4.17 %
44
91.67 %
2
4.17 %
2
4.17 %
0
0.00 %
PAR-NEW-IN
5
312
1.15 %
12
3.85 %
275
88.14 %
25
8.01 %
24
7.69 %
1
0.32 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
101
0.37 %
3
2.97 %
95
94.06 %
4
3.96 %
3
2.97 %
0
0.00 %
Wales
171
6394
23.52 %
488
7.63 %
5610
87.74 %
354
5.54 %
281
4.39 %
15
0.23 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
1
128
0.47 %
18
14.06 %
107
83.59 %
7
5.47 %
3
2.34 %
0
0.00 %
Scotland
1
128
0.47 %
18
14.06 %
107
83.59 %
7
5.47 %
3
2.34 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Grand Total
Sum:
357
27182
1564
24155
xx-xxxx-xxxx
1389
74
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
5
867
0.58 %
66
7.61 %
766
88.35 %
39
4.50 %
32
3.69 %
3
0.35 %
PAR-CUL-P
3
523
0.35 %
31
5.93 %
457
87.38 %
42
8.03 %
35
6.69 %
0
0.00 %
PAR-PERSIS
8
782
0.53 %
132
xx-xxxx-xxxx %
565
72.25 %
85
10.87 %
76
9.72 %
9
1.15 %
SER-ANOM
1
12
0.01 %
0
0.00 %
9
75.00 %
2
16.67 %
2
16.67 %
1
8.33 %
Corn & Scilly
PAR-CUL-N
65
6,498
4.38 %
293
4.51 %
5622
86.52 %
642
9.88 %
570
8.77 %
13
0.20 %
PAR-CUL-P
11
2,484
1.67 %
183
7.37 %
2151
86.59 %
159
6.40 %
148
5.96 %
2
0.08 %
PAR-PERSIS
3
590
0.40 %
30
5.08 %
532
90.17 %
31
5.25 %
28
4.75 %
0
0.00 %
SER-FLEXI
2
264
0.18 %
10
3.79 %
250
94.70 %
6
2.27 %
4
1.52 %
0
0.00 %
Devon
PAR-CUL-N
209
23,232
15.66 %
1629
7.01 %
20352
87.60 %
1332
5.73 %
1151
4.95 %
100
0.43 %
PAR-CUL-P
8
1,084
0.73 %
64
5.90 %
946
87.27 %
90
8.30 %
73
6.73 %
1
0.09 %
PAR-NEW-IN
10
993
0.67 %
62
6.24 %
834
83.99 %
97
9.77 %
94
9.47 %
3
0.30 %
PAR-OTHER
3
58
0.04 %
4
6.90 %
49
84.48 %
6
10.34 %
5
8.62 %
0
0.00 %
PAR-PERSIS
2
423
0.29 %
22
5.20 %
378
89.36 %
25
5.91 %
23
5.44 %
0
0.00 %
Dorset
PAR-CUL-N
49
4,682
3.16 %
311
6.64 %
4145
88.53 %
271
5.79 %
221
4.72 %
5
0.11 %
Gloucs
PAR-CUL-N
55
5,517
3.72 %
292
5.29 %
4791
86.84 %
462
8.37 %
400
7.25 %
34
0.62 %
PAR-CUL-P
2
189
0.13 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
Heref
PAR-CUL-N
9
596
0.40 %
44
7.38 %
487
81.71 %
48
8.05 %
46
7.72 %
19
3.19 %
PAR-OTHER
2
563
0.38 %
19
3.37 %
483
85.79 %
63
11.19 %
61
10.83 %
0
0.00 %
PAR-PERSIS
2
110
0.07 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
SER-OTHER
1
52
0.04 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
24
4,148
2.80 %
172
4.15 %
3751
90.43 %
241
5.81 %
214
5.16 %
11
0.27 %
PAR-OTHER
5
523
0.35 %
20
3.82 %
488
93.31 %
17
3.25 %
7
1.34 %
8
1.53 %
Somerset excl N
PAR-CUL-N
54
4,677
3.15 %
388
8.30 %
4099
87.64 %
237
5.07 %
180
3.85 %
10
0.21 %
PAR-NEW-IN
2
370
0.25 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.31 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
1
297
0.20 %
13
4.38 %
270
90.91 %
21
7.07 %
13
4.38 %
1
0.34 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Staffs
PAR-CUL-N
50
7,674
5.17 %
306
3.99 %
6857
89.35 %
562
7.32 %
509
6.63 %
2
0.03 %
PAR-CUL-P
3
26
0.02 %
2
7.69 %
18
69.23 %
6
23.08 %
6
23.08 %
0
0.00 %
PAR-NEW-IN
3
44
0.03 %
2
4.55 %
36
81.82 %
7
15.91 %
6
13.64 %
0
0.00 %
Wiltshire
PAR-CUL-N
23
2,205
1.49 %
184
8.34 %
1871
84.85 %
166
7.53 %
143
6.49 %
7
0.32 %
PAR-CUL-P
6
450
0.30 %
19
4.22 %
395
87.78 %
26
5.78 %
18
4.00 %
18
4.00 %
PAR-NEW-IN
2
191
0.13 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
5
639
0.43 %
40
6.26 %
573
89.67 %
24
3.76 %
19
2.97 %
7
1.10 %
SER-FLEXI
2
172
0.12 %
13
7.56 %
148
86.05 %
1
0.58 %
1
0.58 %
10
5.81 %
Worcs
PAR-CUL-N
2
128
0.09 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
England - Edge
Berks
PAR-CUL-P
2
319
0.22 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
12
947
0.64 %
58
6.12 %
811
85.64 %
86
9.08 %
78
8.24 %
0
0.00 %
PAR-PERSIS
3
119
0.08 %
8
6.72 %
108
90.76 %
5
4.20 %
3
2.52 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
9
1,360
0.92 %
30
2.21 %
1283
94.34 %
60
4.41 %
46
3.38 %
1
0.07 %
PAR-PERSIS
2
352
0.24 %
5
1.42 %
330
93.75 %
20
5.68 %
17
4.83 %
0
0.00 %
Cheshire
PAR-CUL-N
41
5,303
3.58 %
134
2.53 %
4941
93.17 %
267
5.03 %
227
4.28 %
1
0.02 %
PAR-NEW-IN
25
3,606
2.43 %
113
3.13 %
3297
91.43 %
234
6.49 %
195
5.41 %
1
0.03 %
PAR-PERSIS
3
287
0.19 %
6
2.09 %
268
93.38 %
15
5.23 %
12
4.18 %
1
0.35 %
Derbyshire
PAR-CUL-N
7
985
0.66 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
76
8,096
5.46 %
352
4.35 %
6953
85.88 %
836
10.33 %
724
8.94 %
67
0.83 %
E Sussex
PAR-CUL-N
4
709
0.48 %
74
10.44 %
586
82.65 %
38
5.36 %
34
4.80 %
15
2.12 %
PAR-NEW-IN
4
56
0.04 %
8
14.29 %
36
64.29 %
14
25.00 %
11
19.64 %
1
1.79 %
Hampshire
PAR-CUL-N
3
220
0.15 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
12
1,892
1.28 %
62
3.28 %
xx-xxxx-xxxx
89.43 %
150
7.93 %
136
7.19 %
2
0.11 %
PAR-PERSIS
1
76
0.05 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
22
2,472
1.67 %
118
4.77 %
2211
89.44 %
164
6.63 %
143
5.78 %
0
0.00 %
PAR-PERSIS
3
910
0.61 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
25
1,670
1.13 %
53
3.17 %
1520
91.02 %
120
7.19 %
97
5.81 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
3
184
0.12 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
1
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Oxon
PAR-NEW-IN
40
3,278
2.21 %
113
3.45 %
2978
90.85 %
206
6.28 %
183
5.58 %
4
0.12 %
PAR-PERSIS
5
791
0.53 %
28
3.54 %
733
92.67 %
35
4.42 %
30
3.79 %
0
0.00 %
Warks
PAR-NEW-IN
41
3,083
2.08 %
124
4.02 %
2770
89.85 %
201
6.52 %
179
5.81 %
10
0.32 %
PAR-PERSIS
2
29
0.02 %
3
10.34 %
18
62.07 %
8
27.59 %
8
27.59 %
0
0.00 %
England - LRA
Cleveland
PAR-NEW-IN
2
194
0.13 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
13
2,162
1.46 %
68
3.15 %
1980
91.58 %
125
5.78 %
104
4.81 %
10
0.46 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.06 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
6
540
0.36 %
18
3.33 %
503
93.15 %
24
4.44 %
19
3.52 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.07 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Lancashire
PAR-NEW-IN
7
605
0.41 %
18
2.98 %
549
90.74 %
35
5.79 %
34
5.62 %
4
0.66 %
Lincs
PAR-NEW-IN
12
1,134
0.76 %
41
3.62 %
1015
89.51 %
81
7.14 %
72
6.35 %
6
0.53 %
N Yorks
PAR-NEW-IN
10
420
0.28 %
27
6.43 %
353
84.05 %
45
10.71 %
39
9.29 %
1
0.24 %
Suffolk
PAR-NEW-IN
4
328
0.22 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.14 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
1044
114111
76.93 %
6095
5.34 %
100724
88.27 %
7886
6.91 %
6874
6.02 %
418
0.37 %
Wales - High TB
Carms
PAR-IR-EX
6
22
0.01 %
9
40.91 %
10
45.45 %
2
9.09 %
2
9.09 %
1
4.55 %
PAR-IR-SEV
13
94
0.06 %
24
25.53 %
65
69.15 %
6
6.38 %
5
5.32 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
15
357
0.24 %
46
12.89 %
290
81.23 %
23
6.44 %
19
5.32 %
2
0.56 %
PAR-PERSIS
17
880
0.59 %
54
6.14 %
790
89.77 %
47
5.34 %
36
4.09 %
0
0.00 %
PAR-RAPID
36
69
0.05 %
22
31.88 %
46
66.67 %
1
1.45 %
1
1.45 %
0
0.00 %
PAR-RECUR
5
245
0.17 %
9
3.67 %
225
91.84 %
13
5.31 %
10
4.08 %
1
0.41 %
SER-FLEXI
4
89
0.06 %
20
22.47 %
68
76.40 %
4
4.49 %
1
1.12 %
0
0.00 %
Ceredigion
PAR-IR-EX
3
7
0.00 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
20
131
0.09 %
36
27.48 %
89
67.94 %
8
6.11 %
5
3.82 %
1
0.76 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
2
39
0.03 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
11
930
0.63 %
60
6.45 %
815
87.63 %
59
6.34 %
42
4.52 %
13
1.40 %
PAR-RAPID
20
57
0.04 %
14
24.56 %
41
71.93 %
3
5.26 %
2
3.51 %
0
0.00 %
PAR-RECUR
1
5
0.00 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
SER-FLEXI
4
147
0.10 %
13
8.84 %
129
87.76 %
5
3.40 %
4
2.72 %
1
0.68 %
Gwent
PAR-IR-SEV
4
23
0.02 %
0
0.00 %
22
95.65 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-OTHER
4
159
0.11 %
9
5.66 %
146
91.82 %
7
4.40 %
4
2.52 %
0
0.00 %
PAR-PERSIS
9
364
0.25 %
24
6.59 %
320
87.91 %
20
5.49 %
19
5.22 %
1
0.27 %
PAR-RAPID
4
12
0.01 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
115
0.08 %
10
8.70 %
100
86.96 %
5
4.35 %
5
4.35 %
0
0.00 %
Pembs
PAR-IR-EX
11
52
0.04 %
10
19.23 %
39
75.00 %
3
5.77 %
3
5.77 %
0
0.00 %
PAR-IR-SEV
56
457
0.31 %
115
25.16 %
326
71.33 %
20
4.38 %
16
3.50 %
0
0.00 %
PAR-OTHER
30
1,290
0.87 %
78
6.05 %
1154
89.46 %
66
5.12 %
55
4.26 %
3
0.23 %
PAR-PERSIS
73
4,477
3.02 %
170
3.80 %
4140
92.47 %
217
4.85 %
160
3.57 %
7
0.16 %
PAR-RAPID
82
254
0.17 %
76
29.92 %
171
67.32 %
12
4.72 %
7
2.76 %
0
0.00 %
PAR-RECUR
19
3,304
2.23 %
145
4.39 %
3054
92.43 %
120
3.63 %
94
2.85 %
11
0.33 %
SER-FLEXI
37
1,279
0.86 %
119
9.30 %
1121
87.65 %
48
3.75 %
32
2.50 %
7
0.55 %
Powys
PAR-IR-EX
8
57
0.04 %
17
29.82 %
36
63.16 %
5
8.77 %
4
7.02 %
0
0.00 %
PAR-IR-SEV
11
75
0.05 %
22
29.33 %
52
69.33 %
3
4.00 %
1
1.33 %
0
0.00 %
PAR-NEW-IN
1
239
0.16 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
16
804
0.54 %
41
5.10 %
714
88.81 %
58
7.21 %
43
5.35 %
6
0.75 %
PAR-PERSIS
27
939
0.63 %
30
3.19 %
816
86.90 %
91
9.69 %
85
9.05 %
8
0.85 %
PAR-RAPID
13
29
0.02 %
3
10.34 %
26
89.66 %
1
3.45 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
452
0.30 %
20
4.42 %
409
90.49 %
22
4.87 %
21
4.65 %
2
0.44 %
SER-FLEXI
6
115
0.08 %
17
14.78 %
89
77.39 %
12
10.43 %
9
7.83 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.69 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
1
18
0.01 %
4
22.22 %
14
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Glamorgan
PAR-OTHER
2
67
0.05 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.09 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.01 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
5
660
0.44 %
65
9.85 %
566
85.76 %
36
5.45 %
26
3.94 %
3
0.45 %
PAR-PERSIS
6
435
0.29 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
3
5
0.00 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.15 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.06 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-OTHER
3
106
0.07 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.05 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
1
4
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.09 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.10 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.31 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
16
116
0.08 %
20
17.24 %
88
75.86 %
10
8.62 %
8
6.90 %
0
0.00 %
PAR-NEW-IN
58
4,779
3.22 %
207
4.33 %
4299
89.96 %
309
6.47 %
266
5.57 %
7
0.15 %
PAR-OTHER
5
106
0.07 %
4
3.77 %
95
89.62 %
10
9.43 %
7
6.60 %
0
0.00 %
PAR-PERSIS
10
1,013
0.68 %
36
3.55 %
924
91.21 %
70
6.91 %
53
5.23 %
0
0.00 %
PAR-RAPID
8
38
0.03 %
10
26.32 %
27
71.05 %
1
2.63 %
1
2.63 %
0
0.00 %
PAR-RECUR
2
116
0.08 %
19
16.38 %
92
79.31 %
5
4.31 %
5
4.31 %
0
0.00 %
SER-FLEXI
8
224
0.15 %
24
10.71 %
192
85.71 %
15
6.70 %
8
3.57 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
14
1,487
1.00 %
50
3.36 %
1321
88.84 %
125
8.41 %
113
7.60 %
3
0.20 %
PAR-PERSIS
1
14
0.01 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
92
0.06 %
6
6.52 %
81
88.04 %
7
7.61 %
4
4.35 %
1
1.09 %
S Glamorgan
PAR-RAPID
2
7
0.00 %
5
71.43 %
2
28.57 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
221
0.15 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
126
0.08 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
15
527
0.36 %
22
4.17 %
457
86.72 %
58
11.01 %
45
8.54 %
3
0.57 %
PAR-RAPID
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
19
0.01 %
5
26.32 %
13
68.42 %
1
5.26 %
1
5.26 %
0
0.00 %
NE Wales
PAR-HERD-S
2
48
0.03 %
2
4.17 %
44
91.67 %
2
4.17 %
2
4.17 %
0
0.00 %
PAR-NEW-IN
29
2,431
1.64 %
122
5.02 %
2120
87.21 %
219
9.01 %
185
7.61 %
4
0.16 %
PAR-OTHER
4
152
0.10 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.03 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
SER-FLEXI
5
607
0.41 %
49
8.07 %
521
85.83 %
43
7.08 %
37
6.10 %
0
0.00 %
Wales
817
33355
22.49 %
2083
6.24 %
29518
88.50 %
2035
6.10 %
1653
4.96 %
101
0.30 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
6
843
0.57 %
113
13.40 %
663
78.65 %
65
7.71 %
58
6.88 %
9
1.07 %
Dumfries & G
PAR-NEW-IN
1
8
0.01 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.01 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
9
867
0.58 %
114
13.15 %
683
78.78 %
67
7.73 %
60
6.92 %
10
1.15 %
Grand Total
Sum:
xx-xxxx-xxxx
8292
130925
9988
8587
529
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol May 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
181
20224
97.9%
1035
5.1%
18040
89.2%
1319
6.5%
1100
5.4%
49
0.2%
Serial/Extended
4
436
2.1%
23
5.3%
398
91.3%
7
1.6%
5
1.1%
10
2.3%
Total
185
20660
100.0%
1058
5.1%
18438
89.2%
1326
6.4%
1105
5.3%
59
0.3%
Scotland
Parallel
1
128
100.0%
18
14.1%
107
83.6%
7
5.5%
3
2.3%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
128
100.0%
18
14.1%
107
83.6%
7
5.5%
3
2.3%
0
0.0 %
Wales
Parallel
155
5609
87.7%
403
7.2%
4933
87.9%
322
5.7%
261
4.7%
12
0.2%
Serial/Extended
16
785
12.3%
85
10.8%
677
86.2%
32
4.1%
20
2.5%
3
0.4%
Total
171
6394
100.0%
488
7.6%
5610
87.7%
354
5.5%
281
4.4%
15
0.2%
GB
Parallel
337
25961
95.5%
1456
5.6%
23080
88.9%
xx-xxxx-xxxx
6.3%
1364
5.3%
61
0.2%
Serial/Extended
20
1221
4.5%
108
8.8%
1075
88.0%
39
3.2%
25
2.0%
13
1.1%
Total
357
27182
100.0%
1564
5.8%
24155
88.9%
xx-xxxx-xxxx
6.2%
1389
5.1%
74
0.3%
185.00
171.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
1038 113611
99.6%
6072
5.3% 100266
88.3%
7876
6.9%
6866
6.0%
407
0.4%
Serial/Extended
6
500
0.4%
23
4.6%
458
91.6%
10
2.0%
8
1.6%
11
2.2%
Total
1044 114111
100.0%
6095
5.3% 100724
88.3%
7886
6.9%
6874
6.0%
418
0.4%
Scotland
Parallel
8
863
99.5%
114
13.2%
680
78.8%
67
7.8%
60
7.0%
9
1.0%
Serial/Extended
1
4
0.5%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
9
867
100.0%
114
13.1%
683
78.8%
67
7.7%
60
6.9%
10
1.2%
Wales
Parallel
744
30642
91.9%
1808
5.9%
27186
88.7%
1898
6.2%
1556
5.1%
92
0.3%
Serial/Extended
73
2713
8.1%
275
10.1%
2332
86.0%
137
5.0%
97
3.6%
9
0.3%
Total
817
33355
100.0%
2083
6.2%
29518
88.5%
2035
6.1%
1653
5.0%
101
0.3%
GB
Parallel
1790 145116
97.8%
7994
5.5% 128132
88.3%
9841
6.8%
8482
5.8%
508
0.4%
Serial/Extended
80
3217
2.2%
298
9.3%
2793
86.8%
147
4.6%
105
3.3%
21
0.7%
Total
xx-xxxx-xxxx
100.0%
8292
5.6% 130925
88.3%
9988
6.7%
8587
5.8%
529
0.4%
#######
817.00
9.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
1221
4.5%
108
8.8%
1075
88.0%
39
3.2 %
25
2.0 %
13
1.1 %
Parallel - Herd or Group Slaughter
2
48
0.2%
2
4.2%
44
91.7%
2
4.2 %
2
4.2 %
0
Parallel - IRs in Exit Strategy for persistent her
9
29
0.1%
11
37.9%
15
51.7%
3
10.3 %
3
10.3 %
0
Parallel New Infection
76
7153
26.3%
323
4.5%
6405
89.5%
495
6.9 %
416
5.8 %
9
0.1 %
Parallel - new infection cull area
114
11922
43.9%
601
5.0%
10715
89.9%
705
5.9 %
567
4.8 %
39
0.3 %
Parallel - Other
15
1080
4.0%
84
7.8%
910
84.3%
91
8.4 %
84
7.8 %
2
0.2 %
Parallel Persistent Infection
28
2918
10.7%
104
3.6%
2661
91.2%
183
6.3 %
143
4.9 %
10
0.3 %
Parallel - persistent infection cull area
10
1501
5.5%
156
10.4%
1241
82.7%
107
7.1 %
103
6.9 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
29
59
0.2%
22
37.3%
34
57.6%
5
8.5 %
3
5.1 %
0
Parallel - Recurrent breakdowns
10
913
3.4%
67
7.3%
816
89.4%
40
4.4 %
30
3.3 %
0
Parallel - severe IRs - in persistent herds
44
338
1.2%
86
25.4%
239
70.7%
17
5.0 %
13
3.8 %
0
Total
357
27182
100.0%
1564
5.8%
24155
88.9%
xx-xxxx-xxxx
6.2 %
1389
5.1 %
74
0.3 %
Table 4a. Summaryof IFN Gamma results by submission reason (May 2021)
England - HRA
Extended Flexible IFN-GAMMA test
4
436
1.6%
23
5.3%
398
91.3%
7
1.6 %
5
1.1 %
10
2.3 %
Parallel New Infection
4
398
1.5%
36
9.0%
299
75.1%
65
16.3 %
63
15.8 %
0
Parallel - new infection cull area
103
10440
38.4%
580
5.6%
9327
89.3%
619
5.9 %
494
4.7 %
39
0.4 %
Parallel - Other
3
585
2.2%
21
3.6%
499
85.3%
67
11.5 %
65
11.1 %
0
Parallel Persistent Infection
4
786
2.9%
37
4.7%
716
91.1%
37
4.7 %
25
3.2 %
8
1.0 %
Parallel - persistent infection cull area
10
1501
5.5%
156
10.4%
1241
82.7%
107
7.1 %
103
6.9 %
1
0.1 %
England - HRA
128
14146
52.0%
853
6.0%
12480
88.2%
902
6.4 %
755
5.3 %
58
0.4 %
England - Edge
Parallel New Infection
40
3811
14.0%
153
4.0%
3443
90.3%
265
7.0 %
215
5.6 %
0
Parallel - new infection cull area
11
1482
5.5%
21
1.4%
1388
93.7%
86
5.8 %
73
4.9 %
0
Parallel Persistent Infection
6
1221
4.5%
31
2.5%
1127
92.3%
73
6.0 %
62
5.1 %
1
0.1 %
England - Edge
57
6514
24.0%
205
3.1%
5958
91.5%
424
6.5 %
350
5.4 %
1
0.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
11
608
2.2%
77
12.7%
511
84.0%
26
4.3 %
17
2.8 %
3
0.5 %
Parallel - IRs in Exit Strategy for persistent her
9
29
0.1%
11
37.9%
15
51.7%
3
10.3 %
3
10.3 %
0
Parallel - Other
7
52
0.2%
9
17.3%
42
80.8%
1
1.9 %
1
1.9 %
0
Parallel Persistent Infection
14
332
1.2%
15
4.5%
285
85.8%
40
12.0 %
31
9.3 %
1
0.3 %
Parallel - Rapid Testing Two-times IRs
27
53
0.2%
18
34.0%
32
60.4%
5
9.4 %
3
5.7 %
0
Parallel - Recurrent breakdowns
5
541
2.0%
38
7.0%
491
90.8%
18
3.3 %
12
2.2 %
0
Parallel - severe IRs - in persistent herds
36
295
1.1%
80
27.1%
205
69.5%
14
4.7 %
10
3.4 %
0
Wales - High TB
109
1910
7.0%
248
13.0%
1581
82.8%
107
5.6 %
77
4.0 %
4
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
76
0.3%
5
6.6%
71
93.4%
2
2.6 %
0
0
Parallel New Infection
21
2277
8.4%
94
4.1%
2081
91.4%
115
5.1 %
97
4.3 %
5
0.2 %
Parallel - Other
5
443
1.6%
54
12.2%
369
83.3%
23
5.2 %
18
4.1 %
2
0.5 %
Parallel Persistent Infection
3
578
2.1%
21
3.6%
532
92.0%
33
5.7 %
25
4.3 %
0
Parallel - Rapid Testing Two-times IRs
2
6
0.0%
4
66.7%
2
33.3%
0
0
0
Parallel - Recurrent breakdowns
5
372
1.4%
29
7.8%
325
87.4%
22
5.9 %
18
4.8 %
0
Parallel - severe IRs - in persistent herds
8
43
0.2%
6
14.0%
34
79.1%
3
7.0 %
3
7.0 %
0
Wales - Intermediate TB
47
3795
14.0%
213
5.6%
3414
90.0%
198
5.2 %
161
4.2 %
7
0.2 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
101
0.4%
3
3.0%
95
94.1%
4
4.0 %
3
3.0 %
0
Parallel - Herd or Group Slaughter
2
48
0.2%
2
4.2%
44
91.7%
2
4.2 %
2
4.2 %
0
Parallel New Infection
10
539
2.0%
22
4.1%
475
88.1%
43
8.0 %
38
7.1 %
4
0.7 %
Parallel Persistent Infection
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
15
689
2.5%
27
3.9%
615
89.3%
49
7.1 %
43
6.2 %
4
0.6 %
Scotland - Scotland
Parallel New Infection
1
128
0.5%
18
14.1%
107
83.6%
7
5.5 %
3
2.3 %
0
Scotland - Scotland
1
128
0.5%
18
14.1%
107
83.6%
7
5.5 %
3
2.3 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Extended Flexible IFN-GAMMA test
77
3149
2.1%
298
9.5%
2730
86.7%
144
4.6 %
102
3.2 %
19
0.6 %
Parallel - Herd or Group Slaughter
10
1088
0.7%
47
4.3%
996
91.5%
46
4.2 %
40
3.7 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herds
30
146
0.1%
39
26.7%
97
66.4%
10
6.8 %
9
6.2 %
1
0.7 %
Parallel New Infection
478
44785
30.2%
1914
4.3%
39697
88.6%
3474
7.8 %
3025
6.8 %
149
0.3 %
Parallel - new infection cull area
602
67476
45.5%
3974
5.9%
59418
88.1%
4444
6.6 %
3860
5.7 %
224
0.3 %
Parallel - Other
98
5351
3.6%
321
6.0%
4699
87.8%
352
6.6 %
307
5.7 %
24
0.4 %
Parallel Persistent Infection
203
14893
10.0%
745
5.0%
13363
89.7%
903
6.1 %
736
4.9 %
49
0.3 %
Parallel - persistent infection cull area
35
5075
3.4%
337
6.6%
4407
86.8%
341
6.7 %
292
5.8 %
39
0.8 %
Parallel - Rapid Testing Two-times IRs
173
480
0.3%
137
28.5%
332
69.2%
18
3.8 %
11
2.3 %
0
Parallel - Recurrent breakdowns
40
4908
3.3%
259
5.3%
4467
91.0%
205
4.2 %
166
3.4 %
16
0.3 %
Parallel - severe IRs - in persistent herds
121
914
0.6%
221
24.2%
656
71.8%
48
5.3 %
36
3.9 %
1
0.1 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
xx-xxxx-xxxx
100.0%
8292
5.6%
130925
88.3%
9988
6.7 %
8587
5.8 %
529
0.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Extended Flexible IFN-GAMMA test
4
436
0.3%
23
5.3%
398
91.3%
7
1.6 %
5
1.1 %
10
2.3 %
Parallel New Infection
17
1598
1.1%
130
8.1%
1339
83.8%
132
8.3 %
126
7.9 %
3
0.2 %
Parallel - new infection cull area
545
60224
40.6%
3697
6.1%
52853
87.8%
4003
6.6 %
3468
5.8 %
206
0.3 %
Parallel - Other
12
1611
1.1%
65
4.0%
1409
87.5%
121
7.5 %
129
8.0 %
8
0.5 %
Parallel Persistent Infection
21
2841
1.9%
244
8.6%
2405
84.7%
202
7.1 %
175
6.2 %
17
0.6 %
Parallel - persistent infection cull area
33
4756
3.2%
334
7.0%
4115
86.5%
331
7.0 %
286
6.0 %
21
0.4 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
267
26472
17.8%
1031
3.9%
23560
89.0%
2074
7.8 %
1795
6.8 %
86
0.3 %
Parallel - new infection cull area
56
7234
4.9%
277
3.8%
6559
90.7%
431
6.0 %
382
5.3 %
16
0.2 %
Parallel Persistent Infection
22
2748
1.9%
81
2.9%
2530
92.1%
158
5.7 %
135
4.9 %
2
0.1 %
Parallel - persistent infection cull area
2
319
0.2%
3
0.9%
292
91.5%
10
3.1 %
6
1.9 %
18
5.6 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
England - LRA
Parallel New Infection
62
5790
3.9%
210
3.6%
5198
89.8%
404
7.0 %
354
6.1 %
28
0.5 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
1044 114111
76.9%
6095
5.3%
100724
88.3%
7886
6.9 %
6874
6.0 %
418
0.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
51
1630
1.1%
169
10.4%
1407
86.3%
69
4.2 %
46
2.8 %
8
0.5 %
Parallel - Herd or Group Slaughter
5
1030
0.7%
44
4.3%
944
91.7%
43
4.2 %
37
3.6 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
28
138
0.1%
36
26.1%
92
66.7%
10
7.2 %
9
6.5 %
1
0.7 %
Parallel New Infection
2
245
0.2%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
69
2716
1.8%
177
6.5%
2396
88.2%
167
6.1 %
132
4.9 %
11
0.4 %
Parallel Persistent Infection
139
7726
5.2%
340
4.4%
7010
90.7%
439
5.7 %
347
4.5 %
29
0.4 %
Parallel - Rapid Testing Two-times IRs
156
423
0.3%
118
27.9%
295
69.7%
17
4.0 %
10
2.4 %
0
Parallel - Recurrent breakdowns
29
4121
2.8%
185
4.5%
3791
92.0%
161
3.9 %
131
3.2 %
14
0.3 %
Parallel - severe IRs - in persistent herds
105
798
0.5%
201
25.2%
568
71.2%
38
4.8 %
28
3.5 %
1
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
15
457
0.3%
52
11.4%
391
85.6%
24
5.3 %
13
2.8 %
1
0.2 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
2
8
0.0%
3
37.5%
5
62.5%
0
0
0
Parallel New Infection
78
6859
4.6%
279
4.1%
6125
89.3%
500
7.3 %
440
6.4 %
15
0.2 %
Parallel - Other
13
872
0.6%
75
8.6%
754
86.5%
56
6.4 %
39
4.5 %
4
0.5 %
Parallel Persistent Infection
19
1535
1.0%
78
5.1%
1378
89.8%
104
6.8 %
79
5.1 %
0
Parallel - Rapid Testing Two-times IRs
15
55
0.0%
18
32.7%
36
65.5%
1
1.8 %
1
1.8 %
0
Parallel - Recurrent breakdowns
11
787
0.5%
74
9.4%
676
85.9%
44
5.6 %
35
4.4 %
2
0.3 %
Parallel - severe IRs - in persistent herds
16
116
0.1%
20
17.2%
88
75.9%
10
8.6 %
8
6.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
7
626
0.4%
54
8.6%
534
85.3%
44
7.0 %
38
6.1 %
0
Parallel - Herd or Group Slaughter
3
49
0.0%
2
4.1%
45
91.8%
2
4.1 %
2
4.1 %
0
Parallel New Infection
44
2958
2.0%
144
4.9%
2577
87.1%
277
9.4 %
230
7.8 %
7
0.2 %
Parallel - Other
4
152
0.1%
4
2.6%
140
92.1%
8
5.3 %
7
4.6 %
1
0.7 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales
817
33355
22.5%
2083
6.2%
29518
88.5%
2035
6.1 %
1653
5.0 %
101
0.3 %
Scotland - Scotland
Parallel New Infection
8
863
0.6%
114
13.2%
680
78.8%
67
7.8 %
60
7.0 %
9
1.0 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
9
867
0.6%
114
13.1%
683
78.8%
67
7.7 %
60
6.9 %
10
1.2 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
0
70
0
1
August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
0
65
0
1
October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
3
1
7
0
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
0
1
11
3
1380
13
83
4
1
March
419
36344
256
1867
36
0
0
0
0
75
0
2
8
5
xx-xxxx-xxxx
52
41
0
1
April
367
28108
211
1571
123
0
0
0
0
99
0
12
0
0
1267
67
0
0
2
May
357
27182
214
1564
563
630
0
0
0
0
7
0
0
0
0
64
3
0
0
0
Totals (last
12 months)
4043
321640
2401
15740
566
1007
0
0
0
0
1184
16
16
70
8
11694
136
726
7
6
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2188
249297
1440
11566
759
0
0
0
0
1047
9
10
30
5
8983
20
72
0
2
Scotland
38
4001
23
198
5
0
0
0
0
31
1
1
9
0
67
12
50
3
0
Wales
1817
68342
938
3976
243
0
0
0
0
106
6
5
31
3
2644
104
604
4
4
Totals
4043
321640
2401
15740
1007
0
0
0
0
1184
16
16
70
8
11694
136
726
7
6
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
Northants
NE Wales
Leics & Rut
Heref
Hampshire
Gwynedd
Gwent
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Berks
Ayrshire
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during May 2021 (% submission per County)
County
%Resampled %Retested
Avon
10.99 %
10.99 %
Ayrshire
2.34 %
5.47 %
Berks
8.33 %
9.04 %
Bucks
Carms
3.38 %
4.57 %
Ceredigion
4.69 %
7.04 %
Cheshire
4.84 %
5.60 %
Corn & Scilly
5.29 %
5.98 %
Derbyshire
5.88 %
8.70 %
Devon
5.13 %
6.20 %
Dorset
3.07 %
4.22 %
Gloucs
7.23 %
9.39 %
Gwent
8.40 %
8.40 %
Gwynedd
6.17 %
7.93 %
Hampshire
33.33 %
33.33 %
Heref
10.85 %
11.21 %
Leics & Rut
5.38 %
6.04 %
NE Wales
4.41 %
5.29 %
Northants
5.75 %
9.20 %
Oxon
4.15 %
4.82 %
Pembs
3.23 %
4.95 %
Powys
7.73 %
8.45 %
S Glamorgan
4.07 %
4.52 %
Shropshire
16.67 %
25.00 %
Somerset excl
4.72 %
6.45 %
Staffs
6.37 %
7.20 %
Warks
7.14 %
7.69 %
Wiltshire
2.32 %
2.90 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20408
1055
5.2%
20660
1058
5.1%
Wales
6304
488
7.7%
6394
488
7.6%
Scotland
128
18
14.1%
128
18
14.1%
GB
26838
1561
5.8%
27182
1564
5.8%
Table 6a (i): Number of animals and submissions tested in May 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
109302
6092
5.6%
114111
6095
5.3%
Wales
31737
2083
6.6%
33355
2083
6.2%
Scotland
394
114
28.9%
867
114
13.1%
GB
141424
8289
5.9%
148333
8292
5.6%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of May
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
13105
616
4.7%
13263
616
4.6%
Wales
3759
240
6.4%
3825
240
6.3%
Scotland
479
2
0.4%
483
2
0.4%
GB
17343
858
4.9%
17571
858
4.9%
Table 6a (ii): Number of animals and submissions tested in May 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of May
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
81721
3385
4.1%
86580
3385
3.9%
Wales
20339
1223
6.0%
21415
1224
5.7%
Scotland
2990
77
2.6%
3145
77
2.4%
GB
105049
4685
4.5%
111140
4686
4.2%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SP
1
1
0
0
Total
1
1
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples May 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
1
1
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
1
1
0
0
PRV-SP
1
1
0
0
Wiltshire
PRV-SE
1
10
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
13
93
4
4.30 %
Isle of Man
SER-IOM
3
3
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
7
87
15
17.2%
BOV-IR-EX
1
12
3
25.0%
Ceredigion
BOV-IR-C
8
63
11
17.5%
North East Wales
BOV-IR-C
6
66
11
16.7%
BOV-OTHER
2
100
1
1.0%
BOV-P
1
52
4
7.7%
OTHER
1
14
2
14.3%
Pembrokeshire
BOV-IR-C
18
185
35
18.9%
BOV-IR-EX
4
23
4
17.4%
BOV-OTHER
3
238
8
3.4%
BOV-P
2
96
3
3.1%
Powys
BOV-IR-C
1
16
3
18.8%
BOV-P
1
7
1
14.3%
South Wales
BOV-IR-C
2
11
1
9.1%
BOV-P
1
67
0
0
Wales
58
1,037
102
9.8%
All GB
58
1,037
102
9.8 %
Table 8a Number of Cattle Antibody Tests May 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
Devon
1
95
6
6.3%
England
2
185
6
3.2%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
14
149
30
20.1%
BOV-IR-EX
3
18
5
27.8%
BOV-OTHER
2
26
2
7.7%
BOV-P
1
26
2
7.7%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
19
160
23
14.4%
BOV-OTHER
6
240
16
6.7%
North East Wales
2
24
6
25.0%
BOV-IR-C
12
146
20
13.7%
BOV-IR-EX
1
4
1
25.0%
BOV-OTHER
2
100
1
1.0%
BOV-P
5
594
13
2.2%
OTHER
1
14
2
14.3%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
46
475
78
16.4%
BOV-IR-EX
8
53
9
17.0%
BOV-OTHER
14
822
46
5.6%
BOV-P
8
242
16
6.6%
OTHER
2
18
1
5.6%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Powys
2
11
1
9.1%
BOV-IR-C
11
73
13
17.8%
BOV-IR-EX
2
35
7
20.0%
BOV-OTHER
2
107
18
16.8%
BOV-P
3
54
3
5.6%
South Wales
4
14
5
35.7%
BOV-IR-C
5
43
2
4.7%
BOV-P
1
67
0
0
Wales
202
3,826
362
9.5%
All GB
204
4,011
368
9.2 %