Monthly Report: Surveillance Project SB4008 IFNg (TC0651 and TC0751) & Antibody (TC0077) tests for bovine tuberculosis-February 2024
Source: environment.data.gov.uk (2025-04-29)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2024. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.
Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).
Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period
Issued 09 March 2024
210
1st February - 29th February 2024

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
February
2024
%
Total 2024
%
Submissions
257
491
Samples
Total (%)
20510
100.00 %
42505
100.00 %
England
15367
74.92 %
27303
64.23 %
Wales
4762
23.22 %
14794
34.81 %
Scotland
381
1.86 %
408
0.96 %
Parallel Tests
PAR-CHR
1
0.00 %
411
0.97 %
PAR-CUL-N
49
0.24 %
380
0.89 %
PAR-CUL-P
485
2.36 %
1384
3.26 %
PAR-HERD-S
35
0.17 %
44
0.10 %
PAR-IR-EX
42
0.20 %
98
0.23 %
PAR-IR-SEV
367
1.79 %
684
1.61 %
PAR-NEW-IN
4847
23.63 %
7902
18.59 %
PAR-OTHER
826
4.03 %
908
2.14 %
PAR-PERSIS
3477
xx-xxxx-xxxx %
8186
19.26 %
PAR-POSTM
0
0.00 %
PAR-RAPID
101
0.49 %
157
0.37 %
PAR-RECUR
9063
44.19 %
20233
47.60 %
Total(% of all samples)
19293
94.07 %
40387
95.02 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
1197
5.84 %
1850
4.35 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
SER-OTHER
20
0.10 %
268
0.63 %
Total(% of all samples)
1217
5.93 %
2118
4.98 %
Total
20510
100.00 %
42505
100.00 %
Retests
Total (% of all samples)
2253
10.98 %
3610
8.49 %
England
xx-xxxx-xxxx
8.26 %
2444
5.75 %
Wales
515
2.51 %
1111
2.61 %
Scotland
44
0.21 %
55
0.13 %
Resamples
Total (% of all samples)
2033
9.91 %
3187
7.50 %
England
1542
7.52 %
2184
5.14 %
Wales
451
2.20 %
952
2.24 %
Scotland
40
0.20 %
51
0.12 %
Rejects
Total (% of all samples)
763
3.72 %
836
1.97 %
England
756
3.69 %
803
1.89 %
Wales
6
0.03 %
32
0.08 %
Scotland
1
0.00 %
1
0.00 %
Table 1. Monthly Totals for February 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-OTHER
Breakdown of
Reason for
submission
PAR-CHR
1
0.0 %
PAR-CUL-N
49
0.2 %
PAR-CUL-P
485
2.4 %
PAR-HERD-S
35
0.2 %
PAR-IR-EX
42
0.2 %
PAR-IR-SEV
367
1.8 %
PAR-NEW-IN
4847
23.6 %
PAR-OTHER
826
4.0 %
PAR-PERSIS
3477
17.0 %
PAR-RAPID
101
0.5 %
PAR-RECUR
9063
44.2 %
SER-FLEXI
1197
5.8 %
SER-OTHER
20
0.1 %
Total
20510
100.00 %
Figure 1. Number of samples submitted in each category during February 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
Wigtown
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Leics & Rut
Lancashire
Heref
Gloucs
E Sussex
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during February 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
1000
2000
3000
4000
5000
6000
Wigtown
West lothian
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
Herts
Heref
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
3
699
3.41 %
89
12.73 %
568
81.26 %
45
6.44 %
42
6.01 %
0
0.00 %
PAR-RECUR
2
112
0.55 %
9
8.04 %
93
83.04 %
13
11.61 %
10
8.93 %
0
0.00 %
Corn & Scilly
PAR-OTHER
2
610
2.97 %
70
11.48 %
486
79.67 %
60
9.84 %
54
8.85 %
0
0.00 %
PAR-PERSIS
3
78
0.38 %
4
5.13 %
37
47.44 %
38
48.72 %
37
47.44 %
0
0.00 %
PAR-RECUR
11
1,430
6.97 %
111
7.76 %
1062
74.27 %
274
19.16 %
255
17.83 %
2
0.14 %
SER-FLEXI
2
35
0.17 %
11
31.43 %
22
62.86 %
2
5.71 %
2
5.71 %
0
0.00 %
Devon
PAR-CUL-N
1
49
0.24 %
1
2.04 %
46
93.88 %
4
8.16 %
2
4.08 %
0
0.00 %
PAR-PERSIS
6
845
4.12 %
49
5.80 %
721
85.33 %
82
9.70 %
73
8.64 %
2
0.24 %
PAR-RECUR
6
817
3.98 %
40
4.90 %
722
88.37 %
59
7.22 %
53
6.49 %
2
0.24 %
Gloucs
PAR-RECUR
6
1,039
5.07 %
32
3.08 %
709
68.24 %
42
4.04 %
39
3.75 %
259
24.93 %
Heref
PAR-CUL-P
1
485
2.36 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
485
100.00 %
PAR-PERSIS
1
80
0.39 %
1
1.25 %
77
96.25 %
2
2.50 %
2
2.50 %
0
0.00 %
PAR-RECUR
4
1,016
4.95 %
48
4.72 %
875
86.12 %
98
9.65 %
92
9.06 %
1
0.10 %
Shropshire
PAR-RECUR
9
669
3.26 %
48
7.17 %
554
82.81 %
71
10.61 %
67
10.01 %
0
0.00 %
SER-FLEXI
2
67
0.33 %
13
19.40 %
41
61.19 %
18
26.87 %
13
19.40 %
0
0.00 %
Somerset excl N
PAR-CHR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
396
1.93 %
8
2.02 %
365
92.17 %
22
5.56 %
21
5.30 %
2
0.51 %
Staffs
PAR-RECUR
4
622
3.03 %
22
3.54 %
535
86.01 %
69
11.09 %
65
10.45 %
0
0.00 %
Wiltshire
PAR-RECUR
5
474
2.31 %
26
5.49 %
414
87.34 %
36
7.59 %
34
7.17 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
2
87
0.42 %
8
9.20 %
76
87.36 %
3
3.45 %
3
3.45 %
0
0.00 %
Bucks
PAR-NEW-IN
2
37
0.18 %
2
5.41 %
32
86.49 %
3
8.11 %
3
8.11 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
1,061
5.17 %
32
3.02 %
792
74.65 %
287
27.05 %
237
22.34 %
0
0.00 %
PAR-PERSIS
4
1,049
5.11 %
39
3.72 %
951
90.66 %
72
6.86 %
59
5.62 %
0
0.00 %
Derbyshire
PAR-RECUR
3
209
1.02 %
8
3.83 %
191
91.39 %
12
5.74 %
10
4.78 %
0
0.00 %
E Sussex
PAR-NEW-IN
3
210
1.02 %
9
4.29 %
185
88.10 %
16
7.62 %
16
7.62 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
1,005
4.90 %
29
2.89 %
854
84.98 %
138
13.73 %
122
12.14 %
0
0.00 %
Table 2a. Summary by County for February 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
5
413
2.01 %
21
5.08 %
337
81.60 %
58
14.04 %
53
12.83 %
2
0.48 %
Notts
PAR-NEW-IN
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
1
248
1.21 %
14
5.65 %
205
82.66 %
31
12.50 %
29
11.69 %
0
0.00 %
PAR-RECUR
1
244
1.19 %
8
3.28 %
209
85.66 %
28
11.48 %
27
11.07 %
0
0.00 %
Warks
PAR-RECUR
4
590
2.88 %
25
4.24 %
487
82.54 %
63
10.68 %
78
13.22 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
160
0.78 %
2
1.25 %
148
92.50 %
10
6.25 %
10
6.25 %
0
0.00 %
SER-OTHER
1
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
4
507
2.47 %
7
1.38 %
465
91.72 %
38
7.50 %
34
6.71 %
1
0.20 %
W Sussex
SER-OTHER
2
13
0.06 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
114
15367
74.92 %
787
5.12 %
12282
79.92 %
xx-xxxx-xxxx
11.02 %
1542
10.03 %
756
4.92 %
Wales - High TB
Carms
PAR-IR-EX
2
7
0.03 %
4
57.14 %
1
14.29 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-IR-SEV
7
39
0.19 %
15
38.46 %
20
51.28 %
6
15.38 %
4
10.26 %
0
0.00 %
PAR-OTHER
2
211
1.03 %
18
8.53 %
181
85.78 %
18
8.53 %
12
5.69 %
0
0.00 %
PAR-PERSIS
2
13
0.06 %
1
7.69 %
10
76.92 %
2
15.38 %
2
15.38 %
0
0.00 %
PAR-RAPID
8
39
0.19 %
18
46.15 %
21
53.85 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
636
3.10 %
6
0.94 %
610
95.91 %
29
4.56 %
20
3.14 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
35
0.17 %
6
17.14 %
27
77.14 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-IR-EX
3
7
0.03 %
6
85.71 %
1
14.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
4
0.02 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
1
16
0.08 %
8
50.00 %
6
37.50 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-IR-SEV
1
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
15
0.07 %
10
66.67 %
5
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
1
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
20
288
1.40 %
88
30.56 %
178
61.81 %
24
8.33 %
22
7.64 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
9
634
3.09 %
45
7.10 %
544
85.80 %
58
9.15 %
45
7.10 %
0
0.00 %
PAR-RAPID
7
15
0.07 %
4
26.67 %
11
73.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
364
1.77 %
13
3.57 %
331
90.93 %
21
5.77 %
20
5.49 %
0
0.00 %
SER-FLEXI
8
831
4.05 %
45
5.42 %
727
87.48 %
67
8.06 %
57
6.86 %
2
0.24 %
Powys
PAR-OTHER
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
PAR-PERSIS
1
22
0.11 %
0
0.00 %
7
31.82 %
15
68.18 %
15
68.18 %
0
0.00 %
PAR-RECUR
5
396
1.93 %
17
4.29 %
366
92.42 %
20
5.05 %
13
3.28 %
0
0.00 %
SER-FLEXI
2
177
0.86 %
26
14.69 %
98
55.37 %
60
33.90 %
53
29.94 %
0
0.00 %
S Wales
PAR-RECUR
2
28
0.14 %
4
14.29 %
22
78.57 %
1
3.57 %
1
3.57 %
1
3.57 %
Wales - Intermediate TB
Ceredigion
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
4
8
0.04 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-NEW-IN
1
7
0.03 %
0
0.00 %
6
85.71 %
2
28.57 %
1
14.29 %
0
0.00 %
PAR-PERSIS
9
55
0.27 %
0
0.00 %
47
85.45 %
8
14.55 %
8
14.55 %
0
0.00 %
PAR-RAPID
3
5
0.02 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-RAPID
1
16
0.08 %
4
25.00 %
10
62.50 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-RECUR
2
21
0.10 %
1
4.76 %
17
80.95 %
4
19.05 %
3
14.29 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
3
12
0.06 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-NEW-IN
7
396
1.93 %
8
2.02 %
333
84.09 %
56
14.14 %
52
13.13 %
3
0.76 %
NE Wales
PAR-IR-SEV
2
17
0.08 %
7
41.18 %
9
52.94 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
3
332
1.62 %
3
0.90 %
237
71.39 %
93
28.01 %
92
27.71 %
0
0.00 %
PAR-RAPID
2
9
0.04 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
SER-FLEXI
1
87
0.42 %
17
19.54 %
54
62.07 %
16
18.39 %
16
18.39 %
0
0.00 %
Wales
140
4762
23.22 %
390
8.19 %
3915
82.21 %
515
10.81 %
451
9.47 %
6
0.13 %
Scotland - Scotland
Wigtown
PAR-NEW-IN
3
381
1.86 %
12
3.15 %
328
86.09 %
44
11.55 %
40
10.50 %
1
0.26 %
Scotland
3
381
1.86 %
12
3.15 %
328
86.09 %
44
11.55 %
40
10.50 %
1
0.26 %
Grand Total
Sum:
257
20510
1189
16525
2253
2033
763
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
3
333
0.78 %
47
14.11 %
264
79.28 %
24
7.21 %
22
6.61 %
0
0.00 %
PAR-PERSIS
4
715
1.68 %
90
12.59 %
582
81.40 %
47
6.57 %
43
6.01 %
0
0.00 %
PAR-RECUR
4
400
0.94 %
21
5.25 %
344
86.00 %
41
10.25 %
35
8.75 %
0
0.00 %
Corn & Scilly
PAR-CUL-P
2
891
2.10 %
35
3.93 %
805
90.35 %
58
6.51 %
51
5.72 %
0
0.00 %
PAR-OTHER
2
610
1.44 %
70
11.48 %
486
79.67 %
60
9.84 %
54
8.85 %
0
0.00 %
PAR-PERSIS
5
909
2.14 %
58
6.38 %
744
81.85 %
120
13.20 %
106
11.66 %
1
0.11 %
PAR-RECUR
18
1,831
4.31 %
145
7.92 %
1386
75.70 %
317
17.31 %
296
16.17 %
4
0.22 %
SER-FLEXI
2
35
0.08 %
11
31.43 %
22
62.86 %
2
5.71 %
2
5.71 %
0
0.00 %
Devon
PAR-CUL-N
3
238
0.56 %
13
5.46 %
211
88.66 %
17
7.14 %
14
5.88 %
0
0.00 %
PAR-CUL-P
2
8
0.02 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-PERSIS
6
845
1.99 %
49
5.80 %
721
85.33 %
82
9.70 %
73
8.64 %
2
0.24 %
PAR-RECUR
15
1,402
3.30 %
65
4.64 %
1250
89.16 %
95
6.78 %
85
6.06 %
2
0.14 %
Dorset
PAR-RECUR
5
1,300
3.06 %
94
7.23 %
1155
88.85 %
54
4.15 %
49
3.77 %
2
0.15 %
SER-FLEXI
3
53
0.12 %
10
18.87 %
42
79.25 %
1
1.89 %
1
1.89 %
0
0.00 %
Gloucs
PAR-RECUR
8
1,303
3.07 %
47
3.61 %
952
73.06 %
50
3.84 %
45
3.45 %
259
19.88 %
Heref
PAR-CUL-N
1
142
0.33 %
13
9.15 %
119
83.80 %
12
8.45 %
10
7.04 %
0
0.00 %
PAR-CUL-P
1
485
1.14 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
485
100.00 %
PAR-PERSIS
1
80
0.19 %
1
1.25 %
77
96.25 %
2
2.50 %
2
2.50 %
0
0.00 %
PAR-RECUR
10
2,004
4.71 %
117
5.84 %
1745
87.08 %
160
7.98 %
141
7.04 %
1
0.05 %
Shropshire
PAR-RECUR
13
1,720
4.05 %
108
6.28 %
1491
86.69 %
125
7.27 %
110
6.40 %
11
0.64 %
SER-FLEXI
2
67
0.16 %
13
19.40 %
41
61.19 %
18
26.87 %
13
19.40 %
0
0.00 %
Somerset excl N
PAR-CHR
2
78
0.18 %
4
5.13 %
73
93.59 %
2
2.56 %
1
1.28 %
0
0.00 %
PAR-RECUR
6
662
1.56 %
25
3.78 %
603
91.09 %
35
5.29 %
32
4.83 %
2
0.30 %
Staffs
PAR-PERSIS
1
219
0.52 %
48
21.92 %
155
70.78 %
24
10.96 %
16
7.31 %
0
0.00 %
PAR-RECUR
8
1,092
2.57 %
38
3.48 %
962
88.10 %
94
8.61 %
88
8.06 %
4
0.37 %
Wiltshire
PAR-RECUR
10
1,616
3.80 %
58
3.59 %
1489
92.14 %
79
4.89 %
69
4.27 %
0
0.00 %
Table 2b. Summary by County for 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - Edge
Berks
PAR-NEW-IN
3
383
0.90 %
25
6.53 %
345
90.08 %
14
3.66 %
10
2.61 %
3
0.78 %
Bucks
PAR-NEW-IN
2
37
0.09 %
2
5.41 %
32
86.49 %
3
8.11 %
3
8.11 %
0
0.00 %
PAR-RECUR
3
220
0.52 %
16
7.27 %
188
85.45 %
18
8.18 %
16
7.27 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
1,061
2.50 %
32
3.02 %
792
74.65 %
287
27.05 %
237
22.34 %
0
0.00 %
PAR-PERSIS
4
1,049
2.47 %
39
3.72 %
951
90.66 %
72
6.86 %
59
5.62 %
0
0.00 %
Derbyshire
PAR-RECUR
6
668
1.57 %
17
2.54 %
610
91.32 %
45
6.74 %
39
5.84 %
2
0.30 %
E Sussex
PAR-NEW-IN
5
592
1.39 %
33
5.57 %
526
88.85 %
35
5.91 %
32
5.41 %
1
0.17 %
Leics & Rut
PAR-NEW-IN
7
1,064
2.50 %
32
3.01 %
908
85.34 %
141
13.25 %
124
11.65 %
0
0.00 %
Northants
PAR-NEW-IN
7
415
0.98 %
21
5.06 %
338
81.45 %
59
14.22 %
54
13.01 %
2
0.48 %
Notts
PAR-NEW-IN
2
41
0.10 %
1
2.44 %
38
92.68 %
3
7.32 %
2
4.88 %
0
0.00 %
Oxon
PAR-HERD-S
1
9
0.02 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-NEW-IN
1
248
0.58 %
14
5.65 %
205
82.66 %
31
12.50 %
29
11.69 %
0
0.00 %
PAR-RECUR
2
284
0.67 %
8
2.82 %
248
87.32 %
29
10.21 %
28
9.86 %
0
0.00 %
Warks
PAR-RECUR
4
590
1.39 %
25
4.24 %
487
82.54 %
63
10.68 %
78
13.22 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
2
33
0.08 %
2
6.06 %
9
27.27 %
5
15.15 %
5
15.15 %
17
51.52 %
Cumbria
PAR-NEW-IN
2
182
0.43 %
2
1.10 %
165
90.66 %
15
8.24 %
15
8.24 %
0
0.00 %
SER-OTHER
1
7
0.02 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Herts
PAR-NEW-IN
2
203
0.48 %
10
4.93 %
180
88.67 %
13
6.40 %
13
6.40 %
0
0.00 %
Kent
SER-OTHER
2
248
0.58 %
1
0.40 %
240
96.77 %
9
3.63 %
6
2.42 %
1
0.40 %
Lancashire
PAR-NEW-IN
2
103
0.24 %
1
0.97 %
98
95.15 %
5
4.85 %
3
2.91 %
1
0.97 %
Lincs
PAR-NEW-IN
2
221
0.52 %
3
1.36 %
207
93.67 %
12
5.43 %
11
4.98 %
0
0.00 %
Norfolk
PAR-NEW-IN
2
87
0.20 %
7
8.05 %
54
62.07 %
24
27.59 %
24
27.59 %
2
2.30 %
N Yorks
PAR-NEW-IN
4
507
1.19 %
7
1.38 %
465
91.72 %
38
7.50 %
34
6.71 %
1
0.20 %
W Sussex
SER-OTHER
2
13
0.03 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
207
27303
64.23 %
1478
5.41 %
22838
83.65 %
2444
8.95 %
2184
8.00 %
803
2.94 %
Wales - High TB
Carms
PAR-IR-EX
4
14
0.03 %
4
28.57 %
7
50.00 %
3
21.43 %
3
21.43 %
0
0.00 %
PAR-IR-SEV
13
105
0.25 %
46
43.81 %
53
50.48 %
6
5.71 %
4
3.81 %
2
1.90 %
PAR-OTHER
3
223
0.52 %
19
8.52 %
192
86.10 %
18
8.07 %
12
5.38 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
6
557
1.31 %
70
12.57 %
450
80.79 %
43
7.72 %
37
6.64 %
0
0.00 %
PAR-RAPID
11
43
0.10 %
19
44.19 %
23
53.49 %
1
2.33 %
1
2.33 %
0
0.00 %
PAR-RECUR
6
2,576
6.06 %
46
1.79 %
2478
96.20 %
73
2.83 %
51
1.98 %
1
0.04 %
Ceredigion
PAR-HERD-S
1
35
0.08 %
6
17.14 %
27
77.14 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-IR-EX
3
7
0.02 %
6
85.71 %
1
14.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
11
0.03 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
7
0.02 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
1
16
0.04 %
8
50.00 %
6
37.50 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-IR-SEV
2
4
0.01 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
72
0.17 %
8
11.11 %
63
87.50 %
1
1.39 %
1
1.39 %
0
0.00 %
PAR-RAPID
5
17
0.04 %
11
64.71 %
6
35.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
89
0.21 %
1
1.12 %
78
87.64 %
11
12.36 %
10
11.24 %
0
0.00 %
Pembs
PAR-IR-EX
7
16
0.04 %
10
62.50 %
6
37.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
41
463
1.09 %
169
36.50 %
265
57.24 %
32
6.91 %
29
6.26 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-PERSIS
18
2,345
5.52 %
189
8.06 %
2004
85.46 %
183
7.80 %
149
6.35 %
3
0.13 %
PAR-RAPID
16
48
0.11 %
14
29.17 %
34
70.83 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
12
1,187
2.79 %
55
4.63 %
1066
89.81 %
81
6.82 %
62
5.22 %
4
0.34 %
SER-FLEXI
9
1,298
3.05 %
75
5.78 %
1120
86.29 %
115
8.86 %
101
7.78 %
2
0.15 %
Powys
PAR-IR-EX
2
16
0.04 %
4
25.00 %
11
68.75 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-IR-SEV
4
39
0.09 %
24
61.54 %
15
38.46 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
73
0.17 %
2
2.74 %
66
90.41 %
7
9.59 %
4
5.48 %
1
1.37 %
PAR-PERSIS
2
206
0.48 %
10
4.85 %
159
77.18 %
37
17.96 %
37
17.96 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
13
1,030
2.42 %
60
5.83 %
926
89.90 %
54
5.24 %
42
4.08 %
2
0.19 %
SER-FLEXI
2
177
0.42 %
26
14.69 %
98
55.37 %
60
33.90 %
53
29.94 %
0
0.00 %
S Wales
PAR-RECUR
2
28
0.07 %
4
14.29 %
22
78.57 %
1
3.57 %
1
3.57 %
1
3.57 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-PERSIS
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.01 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
21
0.05 %
17
80.95 %
4
19.05 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
4
8
0.02 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
29
0.07 %
7
24.14 %
18
62.07 %
4
13.79 %
4
13.79 %
0
0.00 %
PAR-NEW-IN
12
1,280
3.01 %
46
3.59 %
1135
88.67 %
107
8.36 %
92
7.19 %
7
0.55 %
PAR-PERSIS
15
1,184
2.79 %
60
5.07 %
1073
90.63 %
50
4.22 %
45
3.80 %
6
0.51 %
PAR-RAPID
3
5
0.01 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
210
0.49 %
15
7.14 %
177
84.29 %
19
9.05 %
18
8.57 %
0
0.00 %
SER-FLEXI
2
128
0.30 %
21
xx-xxxx-xxxx %
100
78.13 %
8
6.25 %
7
5.47 %
0
0.00 %
Powys
PAR-IR-SEV
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
46
0.11 %
1
2.17 %
42
91.30 %
3
6.52 %
3
6.52 %
0
0.00 %
PAR-RAPID
1
16
0.04 %
4
25.00 %
10
62.50 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-RECUR
2
21
0.05 %
1
4.76 %
17
80.95 %
4
19.05 %
3
14.29 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
8
0.02 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
3
12
0.03 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-NEW-IN
7
396
0.93 %
8
2.02 %
333
84.09 %
56
14.14 %
52
13.13 %
3
0.76 %
SER-FLEXI
1
5
0.01 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
2
17
0.04 %
7
41.18 %
9
52.94 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
5
595
1.40 %
22
3.70 %
471
79.16 %
105
17.65 %
102
17.14 %
0
0.00 %
PAR-RAPID
2
9
0.02 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
SER-FLEXI
1
87
0.20 %
17
19.54 %
54
62.07 %
16
18.39 %
16
18.39 %
0
0.00 %
Wales
280
14794
34.81 %
1137
7.69 %
12673
85.66 %
1111
7.51 %
952
6.44 %
32
0.22 %
Scotland - Scotland
West lothian
PAR-NEW-IN
1
27
0.06 %
0
0.00 %
16
59.26 %
11
40.74 %
11
40.74 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
381
0.90 %
12
3.15 %
328
86.09 %
44
11.55 %
40
10.50 %
1
0.26 %
Scotland
4
408
0.96 %
12
2.94 %
344
84.31 %
55
13.48 %
51
12.50 %
1
0.25 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Grand Total
Sum:
491
42505
2627
35855
3610
3187
836
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol February 2024
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
107
15245
99.2%
763
5.0%
12199
80.0%
1674
11.0%
1527
10.0%
756
5.0%
Serial/Extended
7
122
0.8%
24
19.7%
83
68.0%
20
16.4%
15
12.3%
0
0.0 %
Total
114
15367
100.0%
787
5.1%
12282
79.9%
xx-xxxx-xxxx
11.0%
1542
10.0%
756
4.9%
Scotland
Parallel
3
381
100.0%
12
3.1%
328
86.1%
44
11.5%
40
10.5%
1
0.3%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
3
381
100.0%
12
3.1%
328
86.1%
44
11.5%
40
10.5%
1
0.3%
Wales
Parallel
129
3667
77.0%
302
8.2%
3036
82.8%
372
10.1%
325
8.9%
4
0.1%
Serial/Extended
11
1095
23.0%
88
8.0%
879
80.3%
143
13.1%
126
11.5%
2
0.2%
Total
140
4762
100.0%
390
8.2%
3915
82.2%
515
10.8%
451
9.5%
6
0.1%
GB
Parallel
239
19293
94.1%
1077
5.6%
15563
80.7%
2090
10.8%
1892
9.8%
761
3.9%
Serial/Extended
18
1217
5.9%
112
9.2%
962
79.0%
163
13.4%
141
11.6%
2
0.2%
Total
257
20510
100.0%
1189
5.8%
16525
80.6%
2253
11.0%
2033
9.9%
763
3.7%
114.00
140.00
3.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2024)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
195
26880
98.5%
1443
5.4%
22473
83.6%
2414
9.0%
2162
8.0%
802
3.0%
Serial/Extended
12
423
1.5%
35
8.3%
365
86.3%
30
7.1%
22
5.2%
1
0.2%
Total
207
27303
100.0%
1478
5.4%
22838
83.6%
2444
9.0%
2184
8.0%
803
2.9%
Scotland
Parallel
4
408
100.0%
12
2.9%
344
84.3%
55
13.5%
51
12.5%
1
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
4
408
100.0%
12
2.9%
344
84.3%
55
13.5%
51
12.5%
1
0.2%
Wales
Parallel
265
13099
88.5%
996
7.6%
11298
86.3%
912
7.0%
775
5.9%
30
0.2%
Serial/Extended
15
xx-xxxx-xxxx
11.5%
141
8.3%
1375
81.1%
199
11.7%
177
10.4%
2
0.1%
Total
280
14794
100.0%
1137
7.7%
12673
85.7%
1111
7.5%
952
6.4%
32
0.2%
GB
Parallel
464
40387
95.0%
2451
6.1%
34115
84.5%
3381
8.4%
2988
7.4%
833
2.1%
Serial/Extended
27
2118
5.0%
176
8.3%
1740
82.2%
229
10.8%
199
9.4%
3
0.1%
Total
491
42505
100.0%
2627
6.2%
35855
84.4%
3610
8.5%
3187
7.5%
836
2.0%
207.00
280.00
4.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
15
1197
5.8%
112
9.4%
942
78.7%
163
13.6 %
141
11.8 %
2
0.2 %
Parallel – chronic breakdown
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Herd or Group Slaughter
1
35
0.2%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent her
11
42
0.2%
20
47.6%
18
42.9%
4
9.5 %
4
9.5 %
0
Parallel New Infection
42
4847
23.6%
148
3.1%
4000
82.5%
779
16.1 %
692
14.3 %
7
0.1 %
Parallel - new infection cull area
1
49
0.2%
1
2.0%
46
93.9%
4
8.2 %
2
4.1 %
0
Parallel - Other
6
826
4.0%
88
10.7%
670
81.1%
80
9.7 %
68
8.2 %
0
Parallel Persistent Infection
39
3477
17.0%
228
6.6%
2964
85.2%
322
9.3 %
283
8.1 %
2
0.1 %
Parallel - persistent infection cull area
1
485
2.4%
0
0.0 %
0
0.0 %
0
0
485
100.0 %
Parallel - Rapid Testing Two-times IRs
27
101
0.5%
40
39.6%
57
56.4%
4
4.0 %
4
4.0 %
0
Parallel - Recurrent breakdowns
72
9063
44.2%
426
4.7%
7562
83.4%
862
9.5 %
808
8.9 %
267
2.9 %
Parallel - severe IRs - in persistent herds
38
367
1.8%
120
32.7%
218
59.4%
33
9.0 %
29
7.9 %
0
Serial - Other
3
20
0.1%
0
0.0 %
20
100.0%
0
0
0
Total
257
20510
100.0%
1189
5.8%
16525
80.6%
2253
11.0 %
2033
9.9 %
763
3.7 %
Table 4a. Summaryof IFN Gamma results by submission reason (February 2024)
England - HRA
Extended Flexible IFN-GAMMA test
4
102
0.5%
24
23.5%
63
61.8%
20
19.6 %
15
14.7 %
0
Parallel – chronic breakdown
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - new infection cull area
1
49
0.2%
1
2.0%
46
93.9%
4
8.2 %
2
4.1 %
0
Parallel - Other
2
610
3.0%
70
11.5%
486
79.7%
60
9.8 %
54
8.9 %
0
Parallel Persistent Infection
13
1702
8.3%
143
8.4%
1403
82.4%
167
9.8 %
154
9.0 %
2
0.1 %
Parallel - persistent infection cull area
1
485
2.4%
0
0.0 %
0
0.0 %
0
0
485
100.0 %
Parallel - Recurrent breakdowns
49
6575
32.1%
344
5.2%
5329
81.0%
684
10.4 %
636
9.7 %
266
4.0 %
England - HRA
71
9524
46.4%
582
6.1%
7328
76.9%
935
9.8 %
861
9.0 %
753
7.9 %
England - Edge
Parallel New Infection
22
3063
14.9%
116
3.8%
2482
81.0%
536
17.5 %
463
15.1 %
2
0.1 %
Parallel Persistent Infection
4
1049
5.1%
39
3.7%
951
90.7%
72
6.9 %
59
5.6 %
0
Parallel - Recurrent breakdowns
8
1043
5.1%
41
3.9%
887
85.0%
103
9.9 %
115
11.0 %
0
England - Edge
34
5155
25.1%
196
3.8%
4320
83.8%
711
13.8 %
637
12.4 %
2
0.0 %
England - LRA
Parallel New Infection
6
668
3.3%
9
1.3%
614
91.9%
48
7.2 %
44
6.6 %
1
0.1 %
Serial - Other
3
20
0.1%
0
0.0 %
20
100.0%
0
0
0
England - LRA
9
688
3.4%
9
1.3%
634
92.2%
48
7.0 %
44
6.4 %
1
0.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
10
1008
4.9%
71
7.0%
825
81.8%
127
12.6 %
110
10.9 %
2
0.2 %
Parallel - Herd or Group Slaughter
1
35
0.2%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent her
7
34
0.2%
19
55.9%
11
32.4%
4
11.8 %
4
11.8 %
0
Parallel - Other
4
216
1.1%
18
8.3%
184
85.2%
20
9.3 %
14
6.5 %
0
Parallel Persistent Infection
13
671
3.3%
46
6.9%
563
83.9%
75
11.2 %
62
9.2 %
0
Parallel - Rapid Testing Two-times IRs
21
71
0.3%
32
45.1%
39
54.9%
0
0
0
Parallel - Recurrent breakdowns
13
1424
6.9%
40
2.8%
1329
93.3%
71
5.0 %
54
3.8 %
1
0.1 %
Parallel - severe IRs - in persistent herds
30
334
1.6%
107
32.0%
201
60.2%
30
9.0 %
26
7.8 %
0
Wales - High TB
99
3793
18.5%
339
8.9%
3179
83.8%
329
8.7 %
272
7.2 %
3
0.1 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
4
8
0.0%
1
12.5%
7
87.5%
0
0
0
Parallel New Infection
1
7
0.0%
0
0.0 %
6
85.7%
2
28.6 %
1
14.3 %
0
Parallel Persistent Infection
9
55
0.3%
0
0.0 %
47
85.5%
8
14.5 %
8
14.5 %
0
Parallel - Rapid Testing Two-times IRs
4
21
0.1%
5
23.8%
14
66.7%
2
9.5 %
2
9.5 %
0
Parallel - Recurrent breakdowns
2
21
0.1%
1
4.8%
17
81.0%
4
19.0 %
3
14.3 %
0
Parallel - severe IRs - in persistent herds
3
4
0.0%
0
0.0 %
3
75.0%
1
25.0 %
1
25.0 %
0
Wales - Intermediate TB
23
116
0.6%
7
6.0%
94
81.0%
17
14.7 %
15
12.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
87
0.4%
17
19.5%
54
62.1%
16
18.4 %
16
18.4 %
0
Parallel New Infection
10
728
3.5%
11
1.5%
570
78.3%
149
20.5 %
144
19.8 %
3
0.4 %
Parallel - Rapid Testing Two-times IRs
2
9
0.0%
3
33.3%
4
44.4%
2
22.2 %
2
22.2 %
0
Parallel - severe IRs - in persistent herds
5
29
0.1%
13
44.8%
14
48.3%
2
6.9 %
2
6.9 %
0
Wales - Low TB
18
853
4.2%
44
5.2%
642
75.3%
169
19.8 %
164
19.2 %
3
0.4 %
Scotland - Scotland
Parallel New Infection
3
381
1.9%
12
3.1%
328
86.1%
44
11.5 %
40
10.5 %
1
0.3 %
Scotland - Scotland
3
381
1.9%
12
3.1%
328
86.1%
44
11.5 %
40
10.5 %
1
0.3 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
22
1850
4.4%
175
9.5%
1480
80.0%
220
11.9 %
193
10.4 %
2
0.1 %
Parallel – chronic breakdown
5
411
1.0%
51
12.4%
337
82.0%
26
6.3 %
23
5.6 %
0
Parallel - Herd or Group Slaughter
2
44
0.1%
6
13.6%
34
77.3%
4
9.1 %
4
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herds
22
98
0.2%
50
51.0%
42
42.9%
6
6.1 %
6
6.1 %
0
Parallel New Infection
74
7902
18.6%
281
3.6%
6687
84.6%
1011
12.8 %
896
11.3 %
38
0.5 %
Parallel - new infection cull area
4
380
0.9%
26
6.8%
330
86.8%
29
7.6 %
24
6.3 %
0
Parallel - Other
9
908
2.1%
91
10.0%
745
82.0%
86
9.5 %
71
7.8 %
1
0.1 %
Parallel Persistent Infection
68
8186
19.3%
623
7.6%
6983
85.3%
661
8.1 %
568
6.9 %
12
0.1 %
Parallel - persistent infection cull area
5
1384
3.3%
35
2.5%
811
58.6%
60
4.3 %
53
3.8 %
485
35.0 %
Parallel - Rapid Testing Two-times IRs
46
157
0.4%
57
36.3%
94
59.9%
6
3.8 %
6
3.8 %
0
Parallel - Recurrent breakdowns
152
20233
47.6%
966
4.8%
17674
87.4%
1448
7.2 %
1298
6.4 %
295
1.5 %
Parallel - severe IRs - in persistent herds
77
684
1.6%
265
38.7%
378
55.3%
44
6.4 %
39
5.7 %
2
0.3 %
Serial - Other
5
268
0.6%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
Total
491
42505
100.0%
2627
6.2%
35855
84.4%
3610
8.5 %
3187
7.5 %
836
2.0 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2024)
England - HRA
Extended Flexible IFN-GAMMA test
7
155
0.4%
34
21.9%
105
67.7%
21
13.5 %
16
10.3 %
0
Parallel – chronic breakdown
5
411
1.0%
51
12.4%
337
82.0%
26
6.3 %
23
5.6 %
0
Parallel - new infection cull area
4
380
0.9%
26
6.8%
330
86.8%
29
7.6 %
24
6.3 %
0
Parallel - Other
2
610
1.4%
70
11.5%
486
79.7%
60
9.8 %
54
8.9 %
0
Parallel Persistent Infection
17
2768
6.5%
246
8.9%
2279
82.3%
275
9.9 %
240
8.7 %
3
0.1 %
Parallel - persistent infection cull area
5
1384
3.3%
35
2.5%
811
58.6%
60
4.3 %
53
3.8 %
485
35.0 %
Parallel - Recurrent breakdowns
97
13330
31.4%
718
5.4%
11377
85.3%
1050
7.9 %
950
7.1 %
285
2.1 %
England - Edge
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
7
77.8%
2
22.2 %
2
22.2 %
0
Parallel New Infection
29
3841
9.0%
160
4.2%
3184
82.9%
573
14.9 %
491
12.8 %
6
0.2 %
Parallel Persistent Infection
4
1049
2.5%
39
3.7%
951
90.7%
72
6.9 %
59
5.6 %
0
Parallel - Recurrent breakdowns
15
1762
4.1%
66
3.7%
1533
87.0%
155
8.8 %
161
9.1 %
2
0.1 %
England - LRA
Parallel New Infection
16
1336
3.1%
32
2.4%
1178
88.2%
112
8.4 %
105
7.9 %
21
1.6 %
Serial - Other
5
268
0.6%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2024) Cont.
England
207
27303
64.2%
1478
5.4%
22838
83.6%
2444
9.0 %
2184
8.0 %
803
2.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
11
1475
3.5%
101
6.8%
1218
82.6%
175
11.9 %
154
10.4 %
2
0.1 %
Parallel - Herd or Group Slaughter
1
35
0.1%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent herd
17
69
0.2%
32
46.4%
31
44.9%
6
8.7 %
6
8.7 %
0
Parallel - Other
7
298
0.7%
21
7.0%
259
86.9%
26
8.7 %
17
5.7 %
1
0.3 %
Parallel Persistent Infection
30
3183
7.5%
277
8.7%
2679
84.2%
264
8.3 %
224
7.0 %
3
0.1 %
Parallel - Rapid Testing Two-times IRs
37
116
0.3%
46
39.7%
69
59.5%
1
0.9 %
1
0.9 %
0
Parallel - Recurrent breakdowns
35
4910
11.6%
166
3.4%
4570
93.1%
220
4.5 %
166
3.4 %
8
0.2 %
Parallel - severe IRs - in persistent herds
63
622
1.5%
244
39.2%
343
55.1%
38
6.1 %
33
5.3 %
2
0.3 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
128
0.3%
21
16.4%
100
78.1%
8
6.3 %
7
5.5 %
0
Parallel - IRs in Exit Strategy for persistent herd
5
29
0.1%
18
62.1%
11
37.9%
0
0
0
Parallel New Infection
13
1326
3.1%
47
3.5%
1177
88.8%
110
8.3 %
95
7.2 %
7
0.5 %
Parallel Persistent Infection
17
1186
2.8%
61
5.1%
1074
90.6%
50
4.2 %
45
3.8 %
6
0.5 %
Parallel - Rapid Testing Two-times IRs
7
32
0.1%
8
25.0%
21
65.6%
3
9.4 %
3
9.4 %
0
Parallel - Recurrent breakdowns
5
231
0.5%
16
6.9%
194
84.0%
23
10.0 %
21
9.1 %
0
Parallel - severe IRs - in persistent herds
9
33
0.1%
8
24.2%
21
63.6%
4
12.1 %
4
12.1 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
92
0.2%
19
20.7%
57
62.0%
16
17.4 %
16
17.4 %
0
Parallel New Infection
12
991
2.3%
30
3.0%
804
81.1%
161
16.2 %
154
15.5 %
3
0.3 %
Parallel - Rapid Testing Two-times IRs
2
9
0.0%
3
33.3%
4
44.4%
2
22.2 %
2
22.2 %
0
Parallel - severe IRs - in persistent herds
5
29
0.1%
13
44.8%
14
48.3%
2
6.9 %
2
6.9 %
0
Wales
280
14794
34.8%
1137
7.7%
12673
85.7%
1111
7.5 %
952
6.4 %
32
0.2 %
Scotland - Scotland
Parallel New Infection
4
408
1.0%
12
2.9%
344
84.3%
55
13.5 %
51
12.5 %
1
0.2 %
Scotland
4
408
1.0%
12
2.9%
344
84.3%
55
13.5 %
51
12.5 %
1
0.2 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2023 March
266
19894
159
867
9
0
0
0
0
76
0
0
11
0
703
1
67
0
0
April
203
16194
118
782
1
10
0
0
0
0
53
6
0
11
0
634
0
58
1
4
May
208
13834
112
582
4
0
0
0
0
44
2
0
2
0
493
0
37
0
0
June
189
xx-xxxx-xxxx
94
524
2
0
0
0
0
33
2
0
9
0
447
0
31
0
0
July
209
18470
108
597
15
0
0
0
0
81
4
0
10
0
438
0
47
1
0
August
252
20681
140
867
7
0
0
0
0
95
6
0
19
0
675
0
65
0
0
September
221
18070
112
638
10
0
0
0
0
52
2
0
12
0
503
0
56
1
0
October
253
18656
139
825
1
7
0
0
0
0
50
2
0
9
0
701
0
53
0
2
November
250
22290
157
1277
6
29
0
0
0
0
142
5
1
15
5
977
0
97
0
0
December
183
14037
114
892
12
0
0
0
0
69
0
5
15
1
746
17
24
0
3
2024 January
234
21995
171
1438
1
42
0
0
0
0
52
1
6
3
0
1243
81
6
0
3
February
257
20510
159
1189
198
537
0
0
0
0
12
0
6
0
0
188
8
4
0
0
Totals (last
12 months)
2725
221112
1583
10478
207
684
0
0
0
0
759
30
18
116
6
7748
107
545
3
12
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1044
144659
706
6389
534
0
0
0
0
683
17
5
61
3
4812
1
28
1
0
Scotland
84
10602
51
325
6
0
0
0
0
50
0
0
6
0
203
1
42
1
0
Wales
1597
65851
826
3764
144
0
0
0
0
26
13
13
49
3
2733
105
475
1
12
Totals
2725
221112
1583
10478
684
0
0
0
0
759
30
18
116
6
7748
107
545
3
12
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
101
108
112
140
109
112
114
139
93
157
69
114
63
171
98
159
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2022
2023
2024
IFNg Positive herds
IFNg Negative herds
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
19561
867
17206
638
17659
825
20756
1277
13035
892
20308
1434
18508
1189
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2022
2023
2024
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
10
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2022
2023
2024
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
35
40
45
50
W Sussex
Wigtown
S Wales
Somerset excl N
Powys
Oxon
Notts
NE Wales
Lancashire
Gwynedd
Gloucs
Devon
Cumbria
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during February 2024 (% submission per
County)
County
%Resampled %Retested
Avon
6.41 %
7.15 %
Berks
3.45 %
3.45 %
Bucks
8.11 %
8.11 %
Carms
4.23 %
6.03 %
Ceredigion
3.92 %
3.92 %
Cheshire
14.03 %
17.01 %
Corn & Scilly
16.16 %
17.37 %
Cumbria
5.99 %
5.99 %
Derbyshire
4.78 %
5.74 %
Devon
7.48 %
8.47 %
E Sussex
7.62 %
7.62 %
Gloucs
3.75 %
4.04 %
Gwent
5.88 %
5.88 %
Gwynedd
12.99 %
13.97 %
Heref
5.95 %
6.33 %
Lancashire
Leics & Rut
12.14 %
13.73 %
NE Wales
23.14 %
23.52 %
Northants
12.83 %
14.04 %
Notts
N Yorks
6.71 %
7.50 %
Oxon
11.38 %
11.99 %
Pembs
6.79 %
8.00 %
Powys
13.68 %
16.04 %
Shropshire
10.87 %
12.09 %
Somerset excl
5.29 %
5.54 %
Staffs
10.45 %
11.09 %
S Wales
3.57 %
3.57 %
Warks
13.22 %
10.68 %
Wigtown
10.50 %
11.55 %
Wiltshire
7.17 %
7.59 %
W Sussex

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
14825
787
5.3%
15367
787
5.1%
Wales
4521
390
8.6%
4762
390
8.2%
Scotland
351
12
3.4%
381
12
3.1%
GB
19697
1189
6.0%
20510
1189
5.8%
Table 6a (i): Number of animals and submissions tested in February 2024
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
26354
1474
5.6%
27303
1478
5.4%
Wales
14129
1137
8.0%
14794
1137
7.7%
Scotland
378
12
3.2%
408
12
2.9%
GB
40860
2623
6.4%
42505
2627
6.2%
Table 6b (i): Number of animals and submissions tested in 2024 Up to end of February
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
11117
555
5.0%
11362
555
4.9%
Wales
5376
418
7.8%
5516
418
7.6%
Scotland
614
63
10.3%
660
63
9.5%
GB
17107
1036
6.1%
17538
1036
5.9%
Table 6a (ii): Number of animals and submissions tested in February 2023
Table 6b (ii): Number of animals and submissions tested in 2023 Up to end of February
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
25417
1130
4.4%
26902
1130
4.2%
Wales
11963
887
7.4%
12744
887
7.0%
Scotland
1452
157
10.8%
1588
157
9.9%
GB
38816
2174
5.6%
41234
2174
5.3%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
North Yorkshire
PRV-SP
1
1
0
0
Total
2
2
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples February 2024
Table 7b Number of private samples 2024
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
East Sussex
PRV-SP
1
1
0
0
North Yorkshire
PRV-SP
1
1
0
0
Oxfordshire
PRV-SE
1
5
0
0
Total
4
8
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Cumbria
BOV-OTHER
4
885
22
2.5%
England
4
885
22
2.5%
Wales
Carmarthenshire
BOV-IR-C
6
38
7
18.4%
BOV-IR-EX
1
1
0
0
BOV-OTHER
1
7
0
0
Ceredigion
BOV-IR-C
3
11
1
9.1%
BOV-IR-EX
1
2
1
50.0%
North East Wales
BOV-IR-C
2
7
1
14.3%
Pembrokeshire
BOV-IR-C
16
183
34
18.6%
BOV-IR-EX
2
5
2
40.0%
BOV-OTHER
1
26
0
0
BOV-P
1
33
5
15.2%
Powys
BOV-IR-C
1
1
0
0
Wales
35
314
51
16.2%
All GB
39
1,199
73
6.1 %
Table 8a Number of Cattle Antibody Tests February 2024
Table 8b Number of Cattle Antibody Tests 2024
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cumbria
BOV-OTHER
4
885
22
2.5%
England
4
885
22
2.5%
Wales
Carmarthenshire
BOV-IR-C
11
105
23
21.9%
BOV-IR-EX
1
1
0
0
BOV-OTHER
1
7
0
0
Ceredigion
BOV-IR-C
4
14
1
7.1%
BOV-IR-EX
2
23
4
17.4%
North East Wales
BOV-IR-C
3
24
7
29.2%
BOV-P
1
52
4
7.7%
North West Wales
BOV-IR-C
1
5
0
0
Pembrokeshire
BOV-IR-C
37
345
52
15.1%
BOV-IR-EX
7
23
3
13.0%
BOV-OTHER
1
26
0
0
BOV-P
1
33
5
15.2%
Powys
BOV-IR-C
5
39
4
10.3%
BOV-IR-EX
2
16
1
6.3%
South Wales
BOV-IR-C
1
5
0
0
Wales
78
718
104
14.5%
All GB
82
1,603
126
7.9 %