Monthly Report: Surveillance Project SB4008 IFNg (TC0651 and TC0751) & Antibody (TC0077) tests for bovine tuberculosis-May 2024
Source: environment.data.gov.uk (2025-04-29)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2024. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.
Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).
Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period
Issued 09 June 2024
213
1st - 31st May 2024

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
May 2024
%
Total 2024
%
Submissions
207
1150
Samples
Total (%)
15411
100.00 %
96761
100.00 %
England
10837
70.32 %
62824
64.93 %
Wales
4326
28.07 %
32820
33.92 %
Scotland
248
1.61 %
1117
1.15 %
Parallel Tests
PAR-CHR
458
2.97 %
869
0.90 %
PAR-CUL-N
0
0.00 %
381
0.39 %
PAR-CUL-P
68
0.44 %
2682
2.77 %
PAR-HERD-S
0
0.00 %
44
0.05 %
PAR-IR-EX
39
0.25 %
170
0.18 %
PAR-IR-SEV
347
2.25 %
1529
1.58 %
PAR-NEW-IN
2141
13.89 %
18474
19.09 %
PAR-OTHER
528
3.43 %
3231
3.34 %
PAR-PERSIS
3017
19.58 %
19677
20.34 %
PAR-POSTM
0
0.00 %
PAR-RAPID
101
0.66 %
369
0.38 %
PAR-RECUR
8063
52.32 %
45951
47.49 %
Total(% of all samples)
14762
95.79 %
93377
96.50 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
649
4.21 %
3116
3.22 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
268
0.28 %
Total(% of all samples)
649
4.21 %
3384
3.50 %
Total
15411
100.00 %
96761
100.00 %
Retests
Total (% of all samples)
900
5.84 %
7383
7.63 %
England
667
4.33 %
5073
5.24 %
Wales
225
1.46 %
2195
2.27 %
Scotland
8
0.05 %
115
0.12 %
Resamples
Total (% of all samples)
717
4.65 %
6365
6.58 %
England
524
3.40 %
4422
4.57 %
Wales
187
1.21 %
1844
1.91 %
Scotland
6
0.04 %
99
0.10 %
Rejects
Total (% of all samples)
49
0.32 %
1116
1.15 %
England
20
0.13 %
922
0.95 %
Wales
28
0.18 %
191
0.20 %
Scotland
1
0.01 %
3
0.00 %
Table 1. Monthly Totals for May 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
458
3.0 %
PAR-CUL-P
68
0.4 %
PAR-IR-EX
39
0.3 %
PAR-IR-SEV
347
2.3 %
PAR-NEW-IN
2141
13.9 %
PAR-OTHER
528
3.4 %
PAR-PERSIS
3017
19.6 %
PAR-RAPID
101
0.7 %
PAR-RECUR
8063
52.3 %
SER-FLEXI
649
4.2 %
Total
15411
100.00 %
Figure 1. Number of samples submitted in each category during May 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
Ayrshire
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Somerset excl N
Shropshire
Oxon
Northants
Norfolk
Lincs
Leics & Rut
Heref
Gloucs
E Sussex
Durham
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during May 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
Wigtown
West lothian
Ayrshire
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Conwy
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
Humberside
Herts
Heref
Hampshire
Gloucs
E Sussex
Durham
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
2
30
0.19 %
0
0.00 %
22
73.33 %
10
33.33 %
8
26.67 %
0
0.00 %
Corn & Scilly
PAR-RECUR
3
299
1.94 %
5
1.67 %
281
93.98 %
16
5.35 %
13
4.35 %
0
0.00 %
Devon
PAR-CHR
5
458
2.97 %
10
2.18 %
411
89.74 %
41
8.95 %
36
7.86 %
1
0.22 %
PAR-CUL-P
1
28
0.18 %
0
0.00 %
24
85.71 %
4
14.29 %
4
14.29 %
0
0.00 %
PAR-OTHER
1
6
0.04 %
0
0.00 %
5
83.33 %
2
33.33 %
1
16.67 %
0
0.00 %
PAR-RECUR
8
1,842
11.95 %
107
5.81 %
1625
88.22 %
133
7.22 %
106
5.75 %
4
0.22 %
Dorset
PAR-PERSIS
1
588
3.82 %
29
4.93 %
552
93.88 %
16
2.72 %
7
1.19 %
0
0.00 %
PAR-RECUR
1
597
3.87 %
39
6.53 %
549
91.96 %
18
3.02 %
9
1.51 %
0
0.00 %
Gloucs
PAR-RECUR
4
746
4.84 %
11
1.47 %
708
94.91 %
41
5.50 %
27
3.62 %
0
0.00 %
Heref
PAR-PERSIS
1
474
3.08 %
24
5.06 %
439
92.62 %
13
2.74 %
11
2.32 %
0
0.00 %
PAR-RECUR
6
686
4.45 %
23
3.35 %
621
90.52 %
50
7.29 %
42
6.12 %
0
0.00 %
Shropshire
PAR-OTHER
2
38
0.25 %
3
7.89 %
31
81.58 %
4
10.53 %
4
10.53 %
0
0.00 %
PAR-PERSIS
1
591
3.83 %
55
9.31 %
528
89.34 %
11
1.86 %
8
1.35 %
0
0.00 %
PAR-RECUR
5
1,464
9.50 %
38
2.60 %
1358
92.76 %
74
5.05 %
55
3.76 %
13
0.89 %
SER-FLEXI
1
16
0.10 %
1
6.25 %
6
37.50 %
9
56.25 %
9
56.25 %
0
0.00 %
Somerset excl N
PAR-CUL-P
2
40
0.26 %
4
10.00 %
32
80.00 %
4
10.00 %
4
10.00 %
0
0.00 %
PAR-OTHER
1
12
0.08 %
0
0.00 %
7
58.33 %
5
41.67 %
5
41.67 %
0
0.00 %
PAR-RECUR
4
273
1.77 %
17
6.23 %
241
88.28 %
18
6.59 %
15
5.49 %
0
0.00 %
SER-FLEXI
1
56
0.36 %
3
5.36 %
52
92.86 %
2
3.57 %
1
1.79 %
0
0.00 %
Wiltshire
PAR-RECUR
1
11
0.07 %
1
9.09 %
10
90.91 %
2
18.18 %
0
0.00 %
0
0.00 %
England - Edge
Bucks
PAR-NEW-IN
1
104
0.67 %
2
1.92 %
96
92.31 %
7
6.73 %
6
5.77 %
0
0.00 %
Cheshire
PAR-OTHER
1
409
2.65 %
4
0.98 %
401
98.04 %
8
1.96 %
4
0.98 %
0
0.00 %
PAR-PERSIS
1
6
0.04 %
1
16.67 %
2
33.33 %
3
50.00 %
3
50.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
1
16
0.10 %
0
0.00 %
11
68.75 %
5
31.25 %
5
31.25 %
0
0.00 %
PAR-RECUR
3
787
5.11 %
18
2.29 %
721
91.61 %
57
7.24 %
48
6.10 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
198
1.28 %
5
2.53 %
170
85.86 %
25
12.63 %
23
11.62 %
0
0.00 %
Table 2a. Summary by County for May 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
2
18
0.12 %
0
0.00 %
14
77.78 %
4
22.22 %
4
22.22 %
0
0.00 %
Northants
PAR-NEW-IN
1
6
0.04 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-RECUR
1
197
1.28 %
1
0.51 %
180
91.37 %
18
9.14 %
16
8.12 %
0
0.00 %
Oxon
PAR-NEW-IN
1
29
0.19 %
2
6.90 %
23
79.31 %
4
13.79 %
4
13.79 %
0
0.00 %
PAR-PERSIS
1
201
1.30 %
15
7.46 %
174
86.57 %
22
10.95 %
12
5.97 %
0
0.00 %
PAR-RECUR
2
23
0.15 %
0
0.00 %
22
95.65 %
3
13.04 %
1
4.35 %
0
0.00 %
Warks
PAR-OTHER
2
23
0.15 %
0
0.00 %
17
73.91 %
6
26.09 %
6
26.09 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
2
324
2.10 %
1
0.31 %
304
93.83 %
20
6.17 %
19
5.86 %
0
0.00 %
Durham
PAR-NEW-IN
2
10
0.06 %
0
0.00 %
9
90.00 %
0
0.00 %
0
0.00 %
1
10.00 %
Lincs
PAR-NEW-IN
3
206
1.34 %
7
3.40 %
195
94.66 %
8
3.88 %
4
1.94 %
0
0.00 %
Norfolk
PAR-NEW-IN
2
25
0.16 %
0
0.00 %
21
84.00 %
3
12.00 %
3
12.00 %
1
4.00 %
England
79
10837
70.32 %
426
3.93 %
9867
91.05 %
667
6.15 %
524
4.84 %
20
0.18 %
Wales - High TB
Carms
PAR-IR-SEV
5
34
0.22 %
8
23.53 %
25
73.53 %
1
2.94 %
1
2.94 %
0
0.00 %
PAR-NEW-IN
1
21
0.14 %
1
4.76 %
19
90.48 %
2
9.52 %
1
4.76 %
0
0.00 %
PAR-PERSIS
3
644
4.18 %
21
3.26 %
614
95.34 %
8
1.24 %
7
1.09 %
2
0.31 %
PAR-RAPID
6
55
0.36 %
20
36.36 %
34
61.82 %
3
5.45 %
1
1.82 %
0
0.00 %
PAR-RECUR
4
425
2.76 %
29
6.82 %
377
88.71 %
20
4.71 %
18
4.24 %
1
0.24 %
Ceredigion
PAR-IR-EX
1
12
0.08 %
5
41.67 %
7
58.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
32
0.21 %
1
3.13 %
25
78.13 %
8
25.00 %
6
18.75 %
0
0.00 %
Gwent
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
10
0.06 %
3
30.00 %
7
70.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
258
1.67 %
1
0.39 %
247
95.74 %
11
4.26 %
10
3.88 %
0
0.00 %
SER-FLEXI
2
384
2.49 %
3
0.78 %
365
95.05 %
19
4.95 %
14
3.65 %
2
0.52 %
Pembs
PAR-IR-EX
4
21
0.14 %
6
28.57 %
15
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
27
213
1.38 %
64
30.05 %
143
67.14 %
8
3.76 %
6
2.82 %
0
0.00 %
PAR-OTHER
2
39
0.25 %
5
12.82 %
30
76.92 %
4
10.26 %
4
10.26 %
0
0.00 %
PAR-PERSIS
7
481
3.12 %
22
4.57 %
424
88.15 %
17
3.53 %
13
2.70 %
22
4.57 %
PAR-RAPID
8
31
0.20 %
12
38.71 %
17
54.84 %
2
6.45 %
2
6.45 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
209
1.36 %
10
4.78 %
186
89.00 %
15
7.18 %
13
6.22 %
0
0.00 %
SER-FLEXI
4
193
1.25 %
14
7.25 %
150
77.72 %
35
18.13 %
29
15.03 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.03 %
0
0.00 %
4
100.00 %
1
25.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
67
0.43 %
33
49.25 %
33
49.25 %
1
1.49 %
1
1.49 %
0
0.00 %
PAR-RAPID
2
4
0.03 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
4
118
0.77 %
4
3.39 %
109
92.37 %
5
4.24 %
5
4.24 %
0
0.00 %
S Wales
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Ceredigion
PAR-IR-SEV
2
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
6
27
0.18 %
7
25.93 %
17
62.96 %
3
11.11 %
3
11.11 %
0
0.00 %
PAR-NEW-IN
4
225
1.46 %
6
2.67 %
201
89.33 %
20
8.89 %
18
8.00 %
0
0.00 %
PAR-OTHER
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-IR-SEV
1
4
0.03 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
304
1.97 %
5
1.64 %
293
96.38 %
7
2.30 %
6
1.97 %
0
0.00 %
PAR-RECUR
2
98
0.64 %
3
3.06 %
94
95.92 %
1
1.02 %
1
1.02 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
297
1.93 %
16
5.39 %
267
89.90 %
17
5.72 %
13
4.38 %
1
0.34 %
NE Wales
PAR-NEW-IN
5
110
0.71 %
1
0.91 %
94
85.45 %
17
15.45 %
15
13.64 %
0
0.00 %
Wales
126
4326
28.07 %
302
6.98 %
3809
88.05 %
225
5.20 %
187
4.32 %
28
0.65 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
2
248
1.61 %
5
2.02 %
236
95.16 %
8
3.23 %
6
2.42 %
1
0.40 %
Scotland
2
248
1.61 %
5
2.02 %
236
95.16 %
8
3.23 %
6
2.42 %
1
0.40 %
Grand Total
Sum:
207
15411
733
13912
900
717
49
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
3
333
0.34 %
47
14.11 %
264
79.28 %
24
7.21 %
22
6.61 %
0
0.00 %
PAR-PERSIS
5
756
0.78 %
91
12.04 %
621
82.14 %
48
6.35 %
44
5.82 %
0
0.00 %
PAR-RECUR
16
2,057
2.13 %
118
5.74 %
1826
88.77 %
138
6.71 %
111
5.40 %
2
0.10 %
Corn & Scilly
PAR-CUL-P
2
891
0.92 %
35
3.93 %
805
90.35 %
58
6.51 %
51
5.72 %
0
0.00 %
PAR-OTHER
3
947
0.98 %
98
10.35 %
766
80.89 %
97
10.24 %
83
8.76 %
0
0.00 %
PAR-PERSIS
11
1,491
1.54 %
124
8.32 %
1218
81.69 %
169
11.33 %
148
9.93 %
1
0.07 %
PAR-RECUR
32
2,749
2.84 %
201
7.31 %
2161
78.61 %
416
15.13 %
383
13.93 %
4
0.15 %
SER-FLEXI
4
45
0.05 %
12
26.67 %
30
66.67 %
2
4.44 %
2
4.44 %
1
2.22 %
Devon
PAR-CHR
5
458
0.47 %
10
2.18 %
411
89.74 %
41
8.95 %
36
7.86 %
1
0.22 %
PAR-CUL-N
4
239
0.25 %
13
5.44 %
212
88.70 %
17
7.11 %
14
5.86 %
0
0.00 %
PAR-CUL-P
5
225
0.23 %
8
3.56 %
112
49.78 %
111
49.33 %
105
46.67 %
0
0.00 %
PAR-OTHER
6
316
0.33 %
10
3.16 %
263
83.23 %
47
14.87 %
43
13.61 %
0
0.00 %
PAR-PERSIS
8
1,066
1.10 %
64
6.00 %
912
85.55 %
97
9.10 %
87
8.16 %
3
0.28 %
PAR-RECUR
40
5,802
6.00 %
323
5.57 %
5034
86.76 %
481
8.29 %
414
7.14 %
31
0.53 %
Dorset
PAR-PERSIS
1
588
0.61 %
29
4.93 %
552
93.88 %
16
2.72 %
7
1.19 %
0
0.00 %
PAR-RECUR
10
2,661
2.75 %
170
6.39 %
2382
89.52 %
129
4.85 %
107
4.02 %
2
0.08 %
SER-FLEXI
5
159
0.16 %
16
10.06 %
139
87.42 %
5
3.14 %
4
2.52 %
0
0.00 %
Gloucs
PAR-RECUR
22
3,053
3.16 %
141
4.62 %
2546
83.39 %
126
4.13 %
107
3.50 %
259
8.48 %
Heref
PAR-CUL-N
1
142
0.15 %
13
9.15 %
119
83.80 %
12
8.45 %
10
7.04 %
0
0.00 %
PAR-CUL-P
1
485
0.50 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
485
100.00 %
PAR-OTHER
1
150
0.16 %
10
6.67 %
131
87.33 %
11
7.33 %
9
6.00 %
0
0.00 %
PAR-PERSIS
2
554
0.57 %
25
4.51 %
516
93.14 %
15
2.71 %
13
2.35 %
0
0.00 %
PAR-RECUR
25
3,975
4.11 %
172
4.33 %
3483
87.62 %
363
9.13 %
319
8.03 %
1
0.03 %
Shropshire
PAR-CUL-P
2
357
0.37 %
39
10.92 %
308
86.27 %
10
2.80 %
10
2.80 %
0
0.00 %
PAR-OTHER
3
47
0.05 %
3
6.38 %
36
76.60 %
8
17.02 %
8
17.02 %
0
0.00 %
PAR-PERSIS
3
770
0.80 %
81
10.52 %
667
86.62 %
27
3.51 %
22
2.86 %
0
0.00 %
Table 2b. Summary by County for 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
31
5,391
5.57 %
227
4.21 %
4840
89.78 %
360
6.68 %
298
5.53 %
26
0.48 %
SER-FLEXI
4
430
0.44 %
87
20.23 %
307
71.40 %
44
10.23 %
36
8.37 %
0
0.00 %
Somerset excl N
PAR-CHR
2
78
0.08 %
4
5.13 %
73
93.59 %
2
2.56 %
1
1.28 %
0
0.00 %
PAR-CUL-P
4
724
0.75 %
24
3.31 %
649
89.64 %
46
6.35 %
40
5.52 %
11
1.52 %
PAR-OTHER
2
162
0.17 %
4
2.47 %
141
87.04 %
18
11.11 %
17
10.49 %
0
0.00 %
PAR-PERSIS
2
116
0.12 %
16
13.79 %
83
71.55 %
18
15.52 %
16
13.79 %
1
0.86 %
PAR-RECUR
17
2,008
2.08 %
126
6.27 %
1746
86.95 %
144
7.17 %
133
6.62 %
3
0.15 %
SER-FLEXI
1
56
0.06 %
3
5.36 %
52
92.86 %
2
3.57 %
1
1.79 %
0
0.00 %
Staffs
PAR-PERSIS
2
233
0.24 %
53
22.75 %
162
69.53 %
27
11.59 %
18
7.73 %
0
0.00 %
PAR-RECUR
17
1,526
1.58 %
60
3.93 %
1351
88.53 %
123
8.06 %
111
7.27 %
4
0.26 %
Wiltshire
PAR-OTHER
1
470
0.49 %
13
2.77 %
442
94.04 %
23
4.89 %
15
3.19 %
0
0.00 %
PAR-RECUR
20
3,689
3.81 %
113
3.06 %
3438
93.20 %
143
3.88 %
118
3.20 %
20
0.54 %
England - Edge
Berks
PAR-NEW-IN
4
386
0.40 %
25
6.48 %
345
89.38 %
17
4.40 %
13
3.37 %
3
0.78 %
Bucks
PAR-NEW-IN
6
319
0.33 %
9
2.82 %
288
90.28 %
24
7.52 %
21
6.58 %
1
0.31 %
PAR-RECUR
3
220
0.23 %
16
7.27 %
188
85.45 %
18
8.18 %
16
7.27 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
1,061
1.10 %
32
3.02 %
792
74.65 %
287
27.05 %
237
22.34 %
0
0.00 %
PAR-OTHER
2
677
0.70 %
15
2.22 %
654
96.60 %
13
1.92 %
8
1.18 %
0
0.00 %
PAR-PERSIS
15
1,898
1.96 %
120
6.32 %
1572
82.82 %
232
12.22 %
206
10.85 %
0
0.00 %
Derbyshire
PAR-NEW-IN
2
27
0.03 %
0
0.00 %
22
81.48 %
5
18.52 %
5
18.52 %
0
0.00 %
PAR-RECUR
9
1,455
1.50 %
35
2.41 %
1331
91.48 %
102
7.01 %
87
5.98 %
2
0.14 %
E Sussex
PAR-NEW-IN
10
1,210
1.25 %
56
4.63 %
1083
89.50 %
83
6.86 %
70
5.79 %
1
0.08 %
Hampshire
PAR-NEW-IN
1
12
0.01 %
0
0.00 %
11
91.67 %
1
8.33 %
1
8.33 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
21
2,748
2.84 %
98
3.57 %
2419
88.03 %
264
9.61 %
230
8.37 %
1
0.04 %
Northants
PAR-NEW-IN
15
1,599
1.65 %
52
3.25 %
1422
88.93 %
106
6.63 %
98
6.13 %
27
1.69 %
PAR-RECUR
1
197
0.20 %
1
0.51 %
180
91.37 %
18
9.14 %
16
8.12 %
0
0.00 %
Notts
PAR-NEW-IN
6
436
0.45 %
12
2.75 %
369
84.63 %
58
13.30 %
55
12.61 %
0
0.00 %
Oxon
PAR-HERD-S
1
9
0.01 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-NEW-IN
4
396
0.41 %
25
6.31 %
326
82.32 %
47
11.87 %
45
11.36 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
1
201
0.21 %
15
7.46 %
174
86.57 %
22
10.95 %
12
5.97 %
0
0.00 %
PAR-RECUR
8
1,288
1.33 %
42
3.26 %
1178
91.46 %
77
5.98 %
64
4.97 %
4
0.31 %
Warks
PAR-OTHER
3
78
0.08 %
15
19.23 %
56
71.79 %
7
8.97 %
7
8.97 %
0
0.00 %
PAR-RECUR
5
627
0.65 %
26
4.15 %
513
81.82 %
73
11.64 %
88
14.04 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
2
33
0.03 %
2
6.06 %
9
27.27 %
5
15.15 %
5
15.15 %
17
51.52 %
Cumbria
PAR-NEW-IN
8
936
0.97 %
19
2.03 %
856
91.45 %
64
6.84 %
60
6.41 %
1
0.11 %
SER-OTHER
1
7
0.01 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Durham
PAR-NEW-IN
4
109
0.11 %
3
2.75 %
95
87.16 %
8
7.34 %
7
6.42 %
4
3.67 %
Herts
PAR-NEW-IN
2
203
0.21 %
10
4.93 %
180
88.67 %
13
6.40 %
13
6.40 %
0
0.00 %
Humberside
PAR-NEW-IN
2
83
0.09 %
0
0.00 %
75
90.36 %
10
12.05 %
8
9.64 %
0
0.00 %
Kent
SER-OTHER
2
248
0.26 %
1
0.40 %
240
96.77 %
9
3.63 %
6
2.42 %
1
0.40 %
Lancashire
PAR-NEW-IN
2
103
0.11 %
1
0.97 %
98
95.15 %
5
4.85 %
3
2.91 %
1
0.97 %
Lincs
PAR-NEW-IN
5
427
0.44 %
10
2.34 %
402
94.15 %
20
4.68 %
15
3.51 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
112
0.12 %
7
6.25 %
75
66.96 %
27
24.11 %
27
24.11 %
3
2.68 %
N Yorks
PAR-NEW-IN
4
507
0.52 %
7
1.38 %
465
91.72 %
38
7.50 %
34
6.71 %
1
0.20 %
W Sussex
SER-OTHER
2
13
0.01 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
480
62824
64.93 %
3237
5.15 %
54243
86.34 %
5073
8.07 %
4422
7.04 %
922
1.47 %
Wales - High TB
Carms
PAR-IR-EX
8
22
0.02 %
7
31.82 %
11
50.00 %
4
18.18 %
4
18.18 %
0
0.00 %
PAR-IR-SEV
34
245
0.25 %
87
35.51 %
148
60.41 %
11
4.49 %
8
3.27 %
2
0.82 %
PAR-NEW-IN
1
21
0.02 %
1
4.76 %
19
90.48 %
2
9.52 %
1
4.76 %
0
0.00 %
PAR-OTHER
5
232
0.24 %
20
8.62 %
198
85.34 %
20
8.62 %
14
6.03 %
0
0.00 %
PAR-PERSIS
18
3,362
3.47 %
183
5.44 %
3068
91.26 %
124
3.69 %
104
3.09 %
7
0.21 %
PAR-RAPID
24
116
0.12 %
47
40.52 %
66
56.90 %
6
5.17 %
3
2.59 %
0
0.00 %
PAR-RECUR
10
3,001
3.10 %
75
2.50 %
2855
95.13 %
93
3.10 %
69
2.30 %
2
0.07 %
SER-FLEXI
3
59
0.06 %
7
11.86 %
50
84.75 %
2
3.39 %
1
1.69 %
1
1.69 %
Ceredigion
PAR-HERD-S
1
35
0.04 %
6
17.14 %
27
77.14 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-IR-EX
5
27
0.03 %
16
59.26 %
11
40.74 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
36
0.04 %
15
41.67 %
21
58.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
4
485
0.50 %
30
6.19 %
418
86.19 %
42
8.66 %
36
7.42 %
1
0.21 %
PAR-RAPID
4
7
0.01 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
77
0.08 %
9
11.69 %
68
88.31 %
1
1.30 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
3
19
0.02 %
8
42.11 %
9
47.37 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-IR-SEV
4
29
0.03 %
11
37.93 %
18
62.07 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
30
0.03 %
2
6.67 %
25
83.33 %
4
13.33 %
3
10.00 %
0
0.00 %
PAR-PERSIS
3
76
0.08 %
8
10.53 %
67
88.16 %
1
1.32 %
1
1.32 %
0
0.00 %
PAR-RAPID
10
44
0.05 %
22
50.00 %
21
47.73 %
2
4.55 %
1
2.27 %
0
0.00 %
PAR-RECUR
9
868
0.90 %
26
3.00 %
811
93.43 %
34
3.92 %
31
3.57 %
0
0.00 %
SER-FLEXI
2
384
0.40 %
3
0.78 %
365
95.05 %
19
4.95 %
14
3.65 %
2
0.52 %
Pembs
PAR-IR-EX
16
46
0.05 %
18
39.13 %
27
58.70 %
1
2.17 %
1
2.17 %
0
0.00 %
PAR-IR-SEV
100
918
0.95 %
323
35.19 %
537
58.50 %
66
7.19 %
58
6.32 %
0
0.00 %
PAR-OTHER
6
48
0.05 %
5
10.42 %
36
75.00 %
7
14.58 %
7
14.58 %
0
0.00 %
PAR-PERSIS
47
6,187
6.39 %
374
6.04 %
5444
87.99 %
311
5.03 %
232
3.75 %
137
2.21 %
PAR-RAPID
40
130
0.13 %
44
33.85 %
78
60.00 %
8
6.15 %
8
6.15 %
0
0.00 %
PAR-RECUR
29
2,936
3.03 %
130
4.43 %
2637
89.82 %
203
6.91 %
160
5.45 %
9
0.31 %
SER-FLEXI
15
1,506
1.56 %
89
5.91 %
1283
85.19 %
153
10.16 %
132
8.76 %
2
0.13 %
Powys
PAR-IR-EX
3
20
0.02 %
4
20.00 %
15
75.00 %
2
10.00 %
1
5.00 %
0
0.00 %
PAR-IR-SEV
18
137
0.14 %
61
44.53 %
72
52.55 %
4
2.92 %
4
2.92 %
0
0.00 %
PAR-OTHER
2
73
0.08 %
2
2.74 %
66
90.41 %
7
9.59 %
4
5.48 %
1
1.37 %
PAR-PERSIS
4
502
0.52 %
28
5.58 %
426
84.86 %
50
9.96 %
48
9.56 %
0
0.00 %
PAR-RAPID
6
11
0.01 %
5
45.45 %
6
54.55 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
18
1,155
1.19 %
64
5.54 %
1040
90.04 %
61
5.28 %
49
4.24 %
2
0.17 %
SER-FLEXI
2
177
0.18 %
26
14.69 %
98
55.37 %
60
33.90 %
53
29.94 %
0
0.00 %
S Wales
PAR-IR-SEV
1
17
0.02 %
4
23.53 %
13
76.47 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
28
0.03 %
4
14.29 %
22
78.57 %
1
3.57 %
1
3.57 %
1
3.57 %
Wales - Intermediate TB
Carms
PAR-PERSIS
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
21
0.02 %
17
80.95 %
4
19.05 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
4
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
6
11
0.01 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
21
102
0.11 %
29
28.43 %
63
61.76 %
10
9.80 %
10
9.80 %
0
0.00 %
PAR-NEW-IN
29
2,263
2.34 %
88
3.89 %
2000
88.38 %
191
8.44 %
165
7.29 %
10
0.44 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
19
1,331
1.38 %
65
4.88 %
1209
90.83 %
56
4.21 %
51
3.83 %
6
0.45 %
PAR-RAPID
7
10
0.01 %
2
20.00 %
8
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
588
0.61 %
18
3.06 %
527
89.63 %
45
7.65 %
43
7.31 %
0
0.00 %
SER-FLEXI
2
128
0.13 %
21
xx-xxxx-xxxx %
100
78.13 %
8
6.25 %
7
5.47 %
0
0.00 %
Powys
PAR-IR-SEV
2
7
0.01 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
417
0.43 %
10
2.40 %
388
93.05 %
21
5.04 %
19
4.56 %
0
0.00 %
PAR-RAPID
2
18
0.02 %
4
22.22 %
11
61.11 %
3
16.67 %
3
16.67 %
0
0.00 %
PAR-RECUR
5
411
0.42 %
26
6.33 %
365
88.81 %
25
6.08 %
20
4.87 %
0
0.00 %
S Glamorgan
PAR-RAPID
3
14
0.01 %
4
28.57 %
10
71.43 %
1
7.14 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
1
28
0.03 %
0
0.00 %
28
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwynedd
PAR-IR-EX
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
12
0.01 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-NEW-IN
34
2,159
2.23 %
63
2.92 %
1899
87.96 %
221
10.24 %
193
8.94 %
4
0.19 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RECUR
1
18
0.02 %
0
0.00 %
18
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
10
0.01 %
3
30.00 %
6
60.00 %
1
10.00 %
1
10.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
23
0.02 %
9
39.13 %
13
56.52 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-NEW-IN
18
1,762
1.82 %
63
3.58 %
1470
83.43 %
253
14.36 %
226
12.83 %
3
0.17 %
PAR-PERSIS
2
59
0.06 %
5
8.47 %
53
89.83 %
1
1.69 %
1
1.69 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
2
9
0.01 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RECUR
1
171
0.18 %
3
1.75 %
148
86.55 %
19
11.11 %
19
11.11 %
1
0.58 %
SER-FLEXI
3
162
0.17 %
30
18.52 %
104
64.20 %
31
19.14 %
28
17.28 %
0
0.00 %
Wales
661
32820
33.92 %
2244
6.84 %
28541
86.96 %
2195
6.69 %
1844
5.62 %
191
0.58 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
2
248
0.26 %
5
2.02 %
236
95.16 %
8
3.23 %
6
2.42 %
1
0.40 %
West lothian
PAR-NEW-IN
1
27
0.03 %
0
0.00 %
16
59.26 %
11
40.74 %
11
40.74 %
0
0.00 %
Wigtown
PAR-NEW-IN
6
842
0.87 %
20
2.38 %
738
87.65 %
96
11.40 %
82
9.74 %
2
0.24 %
Scotland
9
1117
1.15 %
25
2.24 %
990
88.63 %
115
10.30 %
99
8.86 %
3
0.27 %
Grand Total
Sum:
1150
96761
5506
83774
7383
6365
1116
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol May 2024
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
77
10765
99.3%
422
3.9%
9809
91.1%
656
6.1%
514
4.8%
20
0.2%
Serial/Extended
2
72
0.7%
4
5.6%
58
80.6%
11
15.3%
10
13.9%
0
0.0 %
Total
79
10837
100.0%
426
3.9%
9867
91.0%
667
6.2%
524
4.8%
20
0.2%
Scotland
Parallel
2
248
100.0%
5
2.0%
236
95.2%
8
3.2%
6
2.4%
1
0.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
248
100.0%
5
2.0%
236
95.2%
8
3.2%
6
2.4%
1
0.4%
Wales
Parallel
120
3749
86.7%
285
7.6%
3294
87.9%
171
4.6%
144
3.8%
26
0.7%
Serial/Extended
6
577
13.3%
17
2.9%
515
89.3%
54
9.4%
43
7.5%
2
0.3%
Total
126
4326
100.0%
302
7.0%
3809
88.0%
225
5.2%
187
4.3%
28
0.6%
GB
Parallel
199
14762
95.8%
712
4.8%
13339
90.4%
835
5.7%
664
4.5%
47
0.3%
Serial/Extended
8
649
4.2%
21
3.2%
573
88.3%
65
10.0%
53
8.2%
2
0.3%
Total
207
15411
100.0%
733
4.8%
13912
90.3%
900
5.8%
717
4.7%
49
0.3%
79.00
126.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2024)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
461
61866
98.5%
3118
5.0%
53455
86.4%
5011
8.1%
4373
7.1%
920
1.5%
Serial/Extended
19
958
1.5%
119
12.4%
788
82.3%
62
6.5%
49
5.1%
2
0.2%
Total
480
62824
100.0%
3237
5.2%
54243
86.3%
5073
8.1%
4422
7.0%
922
1.5%
Scotland
Parallel
9
1117
100.0%
25
2.2%
990
88.6%
115
10.3%
99
8.9%
3
0.3%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
9
1117
100.0%
25
2.2%
990
88.6%
115
10.3%
99
8.9%
3
0.3%
Wales
Parallel
631
30394
92.6%
2065
6.8%
26535
87.3%
1921
6.3%
1608
5.3%
186
0.6%
Serial/Extended
30
2426
7.4%
179
7.4%
2006
82.7%
274
11.3%
236
9.7%
5
0.2%
Total
661
32820
100.0%
2244
6.8%
28541
87.0%
2195
6.7%
1844
5.6%
191
0.6%
GB
Parallel
1101
93377
96.5%
5208
5.6%
80980
86.7%
7047
7.5%
6080
6.5%
1109
1.2%
Serial/Extended
49
3384
3.5%
298
8.8%
2794
82.6%
336
9.9%
285
8.4%
7
0.2%
Total
1150
96761
100.0%
5506
5.7%
83774
86.6%
7383
7.6%
6365
6.6%
1116
1.2%
480.00
661.00
9.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
8
649
4.2%
21
3.2%
573
88.3%
65
10.0 %
53
8.2 %
2
0.3 %
Parallel – chronic breakdown
5
458
3.0%
10
2.2%
411
89.7%
41
9.0 %
36
7.9 %
1
0.2 %
Parallel - IRs in Exit Strategy for persistent her
7
39
0.3%
11
28.2%
28
71.8%
1
2.6 %
0
0
Parallel New Infection
36
2141
13.9%
51
2.4%
1958
91.5%
148
6.9 %
128
6.0 %
4
0.2 %
Parallel - Other
10
528
3.4%
12
2.3%
492
93.2%
29
5.5 %
24
4.5 %
0
Parallel Persistent Infection
16
3017
19.6%
168
5.6%
2758
91.4%
98
3.2 %
67
2.2 %
24
0.8 %
Parallel - persistent infection cull area
3
68
0.4%
4
5.9%
56
82.4%
8
11.8 %
8
11.8 %
0
Parallel - Rapid Testing Two-times IRs
20
101
0.7%
36
35.6%
62
61.4%
5
5.0 %
3
3.0 %
0
Parallel - Recurrent breakdowns
55
8063
52.3%
307
3.8%
7351
91.2%
492
6.1 %
387
4.8 %
18
0.2 %
Parallel - severe IRs - in persistent herds
47
347
2.3%
113
32.6%
223
64.3%
13
3.7 %
11
3.2 %
0
Total
207
15411
100.0%
733
4.8%
13912
90.3%
900
5.8 %
717
4.7 %
49
0.3 %
Table 4a. Summaryof IFN Gamma results by submission reason (May 2024)
England - HRA
Extended Flexible IFN-GAMMA test
2
72
0.5%
4
5.6%
58
80.6%
11
15.3 %
10
13.9 %
0
Parallel – chronic breakdown
5
458
3.0%
10
2.2%
411
89.7%
41
9.0 %
36
7.9 %
1
0.2 %
Parallel - Other
4
56
0.4%
3
5.4%
43
76.8%
11
19.6 %
10
17.9 %
0
Parallel Persistent Infection
3
1653
10.7%
108
6.5%
1519
91.9%
40
2.4 %
26
1.6 %
0
Parallel - persistent infection cull area
3
68
0.4%
4
5.9%
56
82.4%
8
11.8 %
8
11.8 %
0
Parallel - Recurrent breakdowns
34
5948
38.6%
241
4.1%
5415
91.0%
362
6.1 %
275
4.6 %
17
0.3 %
England - HRA
51
8255
53.6%
370
4.5%
7502
90.9%
473
5.7 %
365
4.4 %
18
0.2 %
England - Edge
Parallel New Infection
8
371
2.4%
9
2.4%
319
86.0%
46
12.4 %
43
11.6 %
0
Parallel - Other
3
432
2.8%
4
0.9%
418
96.8%
14
3.2 %
10
2.3 %
0
Parallel Persistent Infection
2
207
1.3%
16
7.7%
176
85.0%
25
12.1 %
15
7.2 %
0
Parallel - Recurrent breakdowns
6
1007
6.5%
19
1.9%
923
91.7%
78
7.7 %
65
6.5 %
0
England - Edge
19
2017
13.1%
48
2.4%
1836
91.0%
163
8.1 %
133
6.6 %
0
England - LRA
Parallel New Infection
9
565
3.7%
8
1.4%
529
93.6%
31
5.5 %
26
4.6 %
2
0.4 %
England - LRA
9
565
3.7%
8
1.4%
529
93.6%
31
5.5 %
26
4.6 %
2
0.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
6
577
3.7%
17
2.9%
515
89.3%
54
9.4 %
43
7.5 %
2
0.3 %
Parallel - IRs in Exit Strategy for persistent her
7
39
0.3%
11
28.2%
28
71.8%
1
2.6 %
0
0
Parallel New Infection
1
21
0.1%
1
4.8%
19
90.5%
2
9.5 %
1
4.8 %
0
Parallel - Other
2
39
0.3%
5
12.8%
30
76.9%
4
10.3 %
4
10.3 %
0
Parallel Persistent Infection
11
1157
7.5%
44
3.8%
1063
91.9%
33
2.9 %
26
2.2 %
24
2.1 %
Parallel - Rapid Testing Two-times IRs
20
101
0.7%
36
35.6%
62
61.4%
5
5.0 %
3
3.0 %
0
Parallel - Recurrent breakdowns
13
1010
6.6%
44
4.4%
919
91.0%
51
5.0 %
46
4.6 %
1
0.1 %
Parallel - severe IRs - in persistent herds
38
314
2.0%
105
33.4%
201
64.0%
10
3.2 %
8
2.5 %
0
Wales - High TB
98
3258
21.1%
263
8.1%
2837
87.1%
160
4.9 %
131
4.0 %
27
0.8 %
Wales - Intermediate TB
Parallel New Infection
5
529
3.4%
11
2.1%
494
93.4%
27
5.1 %
24
4.5 %
0
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Recurrent breakdowns
2
98
0.6%
3
3.1%
94
95.9%
1
1.0 %
1
1.0 %
0
Parallel - severe IRs - in persistent herds
9
33
0.2%
8
24.2%
22
66.7%
3
9.1 %
3
9.1 %
0
Wales - Intermediate TB
17
661
4.3%
22
3.3%
611
92.4%
31
4.7 %
28
4.2 %
0
Wales - Low TB
Parallel New Infection
11
407
2.6%
17
4.2%
361
88.7%
34
8.4 %
28
6.9 %
1
0.2 %
Wales - Low TB
11
407
2.6%
17
4.2%
361
88.7%
34
8.4 %
28
6.9 %
1
0.2 %
Scotland - Scotland
Parallel New Infection
2
248
1.6%
5
2.0%
236
95.2%
8
3.2 %
6
2.4 %
1
0.4 %
Scotland - Scotland
2
248
1.6%
5
2.0%
236
95.2%
8
3.2 %
6
2.4 %
1
0.4 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
44
3116
3.2%
297
9.5%
2534
81.3%
327
10.5 %
279
9.0 %
6
0.2 %
Parallel – chronic breakdown
10
869
0.9%
61
7.0%
748
86.1%
67
7.7 %
59
6.8 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
44
0.0%
6
13.6%
34
77.3%
4
9.1 %
4
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herds
44
170
0.2%
73
42.9%
89
52.4%
9
5.3 %
8
4.7 %
0
Parallel New Infection
200
18474
19.1%
618
3.3%
16126
87.3%
1885
10.2 %
1650
8.9 %
80
0.4 %
Parallel - new infection cull area
5
381
0.4%
26
6.8%
331
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
39
3231
3.3%
197
6.1%
2815
87.1%
262
8.1 %
218
6.7 %
1
0.0 %
Parallel Persistent Infection
149
19677
20.3%
1312
6.7%
17163
87.2%
1256
6.4 %
1046
5.3 %
156
0.8 %
Parallel - persistent infection cull area
14
2682
2.8%
106
4.0%
1874
69.9%
225
8.4 %
206
7.7 %
496
18.5 %
Parallel - Rapid Testing Two-times IRs
104
369
0.4%
136
36.9%
214
58.0%
24
6.5 %
19
5.1 %
0
Parallel - Recurrent breakdowns
338
45951
47.5%
2126
4.6%
40688
88.5%
3193
6.9 %
2764
6.0 %
373
0.8 %
Parallel - severe IRs - in persistent herds
196
1529
1.6%
547
35.8%
898
58.7%
93
6.1 %
82
5.4 %
2
0.1 %
Serial - Other
5
268
0.3%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
Total
1150
96761
100.0%
5506
5.7%
83774
86.6%
7383
7.6 %
6365
6.6 %
1116
1.2 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2024)
England - HRA
Extended Flexible IFN-GAMMA test
14
690
0.7%
118
17.1%
528
76.5%
53
7.7 %
43
6.2 %
1
0.1 %
Parallel – chronic breakdown
10
869
0.9%
61
7.0%
748
86.1%
67
7.7 %
59
6.8 %
1
0.1 %
Parallel - new infection cull area
5
381
0.4%
26
6.8%
331
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
16
2092
2.2%
138
6.6%
1779
85.0%
204
9.8 %
175
8.4 %
0
Parallel Persistent Infection
34
5574
5.8%
483
8.7%
4731
84.9%
417
7.5 %
355
6.4 %
5
0.1 %
Parallel - persistent infection cull area
14
2682
2.8%
106
4.0%
1874
69.9%
225
8.4 %
206
7.7 %
496
18.5 %
Parallel - Recurrent breakdowns
230
32911
34.0%
1651
5.0%
28807
87.5%
2423
7.4 %
2101
6.4 %
352
1.1 %
England - Edge
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
7
77.8%
2
22.2 %
2
22.2 %
0
Parallel New Infection
71
8194
8.5%
309
3.8%
7077
86.4%
892
10.9 %
775
9.5 %
33
0.4 %
Parallel - Other
5
755
0.8%
30
4.0%
710
94.0%
20
2.6 %
15
2.0 %
0
Parallel Persistent Infection
16
2099
2.2%
135
6.4%
1746
83.2%
254
12.1 %
218
10.4 %
0
Parallel - Recurrent breakdowns
26
3787
3.9%
120
3.2%
3390
89.5%
288
7.6 %
271
7.2 %
6
0.2 %
England - LRA
Parallel New Infection
33
2513
2.6%
59
2.3%
2255
89.7%
190
7.6 %
172
6.8 %
27
1.1 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2024) Cont.
Serial - Other
5
268
0.3%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
England
480
62824
64.9%
3237
5.2%
54243
86.3%
5073
8.1 %
4422
7.0 %
922
1.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
22
2126
2.2%
125
5.9%
1796
84.5%
234
11.0 %
200
9.4 %
5
0.2 %
Parallel - Herd or Group Slaughter
1
35
0.0%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent herd
35
134
0.1%
53
39.6%
73
54.5%
9
6.7 %
8
6.0 %
0
Parallel New Infection
1
21
0.0%
1
4.8%
19
90.5%
2
9.5 %
1
4.8 %
0
Parallel - Other
17
383
0.4%
29
7.6%
325
84.9%
38
9.9 %
28
7.3 %
1
0.3 %
Parallel Persistent Infection
76
10612
11.0%
623
5.9%
9423
88.8%
528
5.0 %
421
4.0 %
145
1.4 %
Parallel - Rapid Testing Two-times IRs
85
309
0.3%
120
38.8%
177
57.3%
16
5.2 %
12
3.9 %
0
Parallel - Recurrent breakdowns
69
8065
8.3%
308
3.8%
7433
92.2%
393
4.9 %
310
3.8 %
14
0.2 %
Parallel - severe IRs - in persistent herds
163
1382
1.4%
501
36.3%
809
58.5%
81
5.9 %
70
5.1 %
2
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
128
0.1%
21
16.4%
100
78.1%
8
6.3 %
7
5.5 %
0
Parallel - IRs in Exit Strategy for persistent herd
7
32
0.0%
19
59.4%
13
40.6%
0
0
0
Parallel New Infection
33
2680
2.8%
98
3.7%
2388
89.1%
212
7.9 %
184
6.9 %
10
0.4 %
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
21
1333
1.4%
66
5.0%
1210
90.8%
56
4.2 %
51
3.8 %
6
0.5 %
Parallel - Rapid Testing Two-times IRs
15
49
0.1%
13
26.5%
32
65.3%
5
10.2 %
4
8.2 %
0
Parallel - Recurrent breakdowns
11
999
1.0%
44
4.4%
892
89.3%
70
7.0 %
63
6.3 %
0
Parallel - severe IRs - in persistent herds
26
112
0.1%
31
27.7%
71
63.4%
10
8.9 %
10
8.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
6
172
0.2%
33
19.2%
110
64.0%
32
18.6 %
29
16.9 %
0
Parallel - IRs in Exit Strategy for persistent herd
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
53
3949
4.1%
126
3.2%
3397
86.0%
474
12.0 %
419
10.6 %
7
0.2 %
Parallel Persistent Infection
2
59
0.1%
5
8.5%
53
89.8%
1
1.7 %
1
1.7 %
0
Parallel - Rapid Testing Two-times IRs
4
11
0.0%
3
27.3%
5
45.5%
3
27.3 %
3
27.3 %
0
Parallel - Recurrent breakdowns
2
189
0.2%
3
1.6%
166
87.8%
19
10.1 %
19
10.1 %
1
0.5 %
Parallel - severe IRs - in persistent herds
7
35
0.0%
15
42.9%
18
51.4%
2
5.7 %
2
5.7 %
0
Wales
661
32820
33.9%
2244
6.8%
28541
87.0%
2195
6.7 %
1844
5.6 %
191
0.6 %
Scotland - Scotland
Parallel New Infection
9
1117
1.2%
25
2.2%
990
88.6%
115
10.3 %
99
8.9 %
3
0.3 %
Scotland
9
1117
1.2%
25
2.2%
990
88.6%
115
10.3 %
99
8.9 %
3
0.3 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2023 June
189
xx-xxxx-xxxx
94
524
2
0
0
0
0
33
2
0
9
0
447
0
31
0
0
July
209
18470
108
597
15
0
0
0
0
81
4
0
10
0
438
0
47
1
0
August
252
20681
140
867
7
0
0
0
0
95
6
0
19
0
675
0
65
0
0
September
221
18070
112
638
10
0
0
0
0
52
2
0
12
0
503
0
56
1
0
October
253
18656
139
825
1
7
0
0
0
0
50
2
0
9
0
701
0
53
0
2
November
250
22290
157
1277
6
24
0
0
0
0
142
8
0
18
0
982
0
97
0
0
December
183
14037
114
892
11
0
0
0
0
69
3
0
18
0
747
0
44
0
0
2024 January
234
21995
171
1438
1
26
0
0
0
0
54
4
1
5
0
1254
1
91
0
1
February
257
20510
159
1189
16
0
0
0
0
31
1
0
12
0
1018
1
109
1
0
March
215
19876
142
1054
15
0
0
0
0
70
0
3
22
3
879
0
60
0
0
April
237
18969
146
1092
60
0
0
0
0
44
0
9
6
0
908
23
42
0
0
May
207
15411
129
733
166
233
0
0
0
0
1
0
6
0
0
12
7
0
0
0
Totals (last
12 months)
2707
225446
1611
11126
174
426
0
0
0
0
722
32
19
140
3
8564
32
695
3
3
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1083
148351
726
6834
215
0
0
0
0
692
17
12
86
1
5390
5
80
1
0
Scotland
52
7142
30
143
6
0
0
0
0
6
0
0
2
0
84
0
37
1
0
Wales
1572
69953
855
4149
205
0
0
0
0
24
15
7
52
2
3090
27
578
1
3
Totals
2707
225446
1611
11126
426
0
0
0
0
722
32
19
140
3
8564
32
695
3
3
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
101
108
112
140
109
112
114
139
93
157
69
114
63
171
98
159
73
142
91
146
78
129
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2022
2023
2024
IFNg Positive herds
IFNg Negative herds
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
19561
867
17206
638
17659
825
20756
1277
13035
892
20308
1434
18508
1189
18373
1054
17402
1092
14468
733
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2022
2023
2024
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
10
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2022
2023
2024
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
Warks
S Wales
Somerset excl N
Shropshire
Powys
Pembs
Oxon
Northants
Norfolk
NE Wales
Lincs
Leics & Rut
Heref
Gwynedd
Gwent
Gloucs
E Sussex
Durham
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Ayrshire
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during May 2024 (% submission per County)
County
%Resampled %Retested
Avon
26.67 %
33.33 %
Ayrshire
2.42 %
3.23 %
Bucks
5.77 %
6.73 %
Carms
2.37 %
2.88 %
Ceredigion
13.04 %
17.39 %
Cheshire
1.69 %
2.65 %
Corn & Scilly
4.35 %
5.35 %
Cumbria
5.86 %
6.17 %
Derbyshire
6.60 %
7.72 %
Devon
6.30 %
7.71 %
Dorset
1.35 %
2.87 %
Durham
E Sussex
11.62 %
12.63 %
Gloucs
3.62 %
5.50 %
Gwent
3.67 %
4.59 %
Gwynedd
4.38 %
5.72 %
Heref
4.57 %
5.43 %
Leics & Rut
22.22 %
22.22 %
Lincs
1.94 %
3.88 %
NE Wales
9.92 %
11.02 %
Norfolk
12.00 %
12.00 %
Northants
8.37 %
9.36 %
Oxon
6.72 %
11.46 %
Pembs
5.64 %
6.82 %
Powys
2.17 %
2.50 %
Shropshire
3.60 %
4.65 %
Somerset excl
6.56 %
7.61 %
S Wales
Warks
26.09 %
26.09 %
Wiltshire
18.18 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
10735
426
4.0%
10837
426
3.9%
Wales
4223
302
7.2%
4326
302
7.0%
Scotland
243
5
2.1%
248
5
2.0%
GB
15201
733
4.8%
15411
733
4.8%
Table 6a (i): Number of animals and submissions tested in May 2024
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
59158
3233
5.5%
62824
3237
5.2%
Wales
31053
2244
7.2%
32820
2244
6.8%
Scotland
1051
25
2.4%
1117
25
2.2%
GB
91259
5502
6.0%
96761
5506
5.7%
Table 6b (i): Number of animals and submissions tested in 2024 Up to end of May
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
9098
374
4.1%
9134
374
4.1%
Wales
3940
198
5.0%
3966
198
5.0%
Scotland
711
10
1.4%
734
10
1.4%
GB
13749
582
4.2%
13834
582
4.2%
Table 6a (ii): Number of animals and submissions tested in May 2023
Table 6b (ii): Number of animals and submissions tested in 2023 Up to end of May
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
55856
2444
4.4%
58731
2444
4.2%
Wales
25099
1609
6.4%
26668
1609
6.0%
Scotland
4105
352
8.6%
5757
352
6.1%
GB
85042
4405
5.2%
91156
4405
4.8%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
3
0
0
Devon
PRV-SE
2
37
1
2.70 %
Total
3
40
1
2.50 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples May 2024
Table 7b Number of private samples 2024
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SE
1
15
0
0
PRV-SP
2
4
0
0
Devon
PRV-SE
4
137
9
6.57 %
East Sussex
PRV-SP
1
1
0
0
Gloucestershire
PRV-SE
1
78
7
8.97 %
North Yorkshire
PRV-SP
1
1
0
0
Oxfordshire
PRV-SE
1
5
0
0
Wiltshire
PRV-SP
1
1
0
0
Worcestershire
PRV-SE
1
1
0
0
Total
13
243
16
6.58 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
4
55
11
20.0%
Ceredigion
BOV-IR-C
2
8
0
0
BOV-IR-EX
1
12
4
33.3%
North East Wales
BOV-IR-C
3
10
5
50.0%
BOV-IR-EX
1
1
1
100.0%
Pembrokeshire
BOV-IR-C
21
221
42
19.0%
BOV-IR-EX
4
21
7
33.3%
BOV-P
1
130
6
4.6%
Powys
BOV-IR-C
6
78
11
14.1%
South Wales
BOV-P
2
196
6
3.1%
Wales
45
732
93
12.7%
All GB
45
732
93
12.7 %
Table 8a Number of Cattle Antibody Tests May 2024
Table 8b Number of Cattle Antibody Tests 2024
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cumbria
BOV-OTHER
4
885
22
2.5%
Oxfordshire
BOV-P
1
114
10
8.8%
England
5
999
32
3.2%
Wales
Carmarthenshire
BOV-IR-C
24
227
42
18.5%
BOV-IR-EX
5
18
6
33.3%
BOV-OTHER
1
7
0
0
BOV-P
1
57
0
0
Ceredigion
BOV-IR-C
9
47
5
10.6%
BOV-IR-EX
6
48
15
31.3%
North East Wales
BOV-IR-C
10
59
15
25.4%
BOV-IR-EX
3
9
2
22.2%
BOV-OTHER
1
9
5
55.6%
BOV-P
3
111
7
6.3%
North West Wales
BOV-IR-C
1
5
0
0
Pembrokeshire
BOV-IR-C
84
909
174
19.1%
BOV-IR-EX
11
44
10
22.7%
BOV-OTHER
2
85
3
3.5%
BOV-P
3
172
12
7.0%
Powys
BOV-IR-C
16
144
21
14.6%
BOV-IR-EX
2
16
1
6.3%
South Wales
BOV-IR-C
5
50
8
16.0%
BOV-IR-EX
1
16
4
25.0%
BOV-P
2
196
6
3.1%
Wales
190
2,229
336
15.1%
All GB
195
3,228
368
11.4 %