Monthly Report: Surveillance Project SB4008 IFNg (TC0651 and TC0751) & Antibody (TC0077) tests for bovine tuberculosis- August  2024
Source: environment.data.gov.uk (2025-04-29)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2024. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.
Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).
Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period
Issued 09 September 2024
216
1st - 31st August 2024

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
August 2024
%
Total 2024
%
Submissions
197
1767
Samples
Total (%)
14635
100.00 %
148991
100.00 %
England
8825
60.30 %
96818
64.98 %
Wales
3847
26.29 %
46999
31.54 %
Scotland
1963
13.41 %
5174
3.47 %
Parallel Tests
PAR-CHR
59
0.40 %
1106
0.74 %
PAR-CUL-N
0
0.00 %
382
0.26 %
PAR-CUL-P
810
5.53 %
5027
3.37 %
PAR-HERD-S
0
0.00 %
44
0.03 %
PAR-IR-EX
23
0.16 %
244
0.16 %
PAR-IR-SEV
252
1.72 %
2350
1.58 %
PAR-NEW-IN
4850
33.14 %
33119
22.23 %
PAR-OTHER
776
5.30 %
4886
3.28 %
PAR-PERSIS
1345
9.19 %
25231
xx-xxxx-xxxx %
PAR-POSTM
0
0.00 %
PAR-RAPID
136
0.93 %
596
0.40 %
PAR-RECUR
5992
40.94 %
69780
46.84 %
Total(% of all samples)
14243
97.32 %
142765
95.82 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
392
2.68 %
5954
4.00 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
268
0.18 %
Total(% of all samples)
392
2.68 %
6226
4.18 %
Total
14635
100.00 %
148991
100.00 %
Retests
Total (% of all samples)
787
5.38 %
10201
6.85 %
England
472
3.23 %
7026
4.72 %
Wales
178
1.22 %
2832
1.90 %
Scotland
137
0.94 %
343
0.23 %
Resamples
Total (% of all samples)
533
3.64 %
8336
5.59 %
England
313
2.14 %
5789
3.89 %
Wales
123
0.84 %
2286
1.53 %
Scotland
97
0.66 %
261
0.18 %
Rejects
Total (% of all samples)
14
0.10 %
1336
0.90 %
England
11
0.08 %
1126
0.76 %
Wales
2
0.01 %
205
0.14 %
Scotland
1
0.01 %
5
0.00 %
Table 1. Monthly Totals for August 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
59
0.4 %
PAR-CUL-P
810
5.5 %
PAR-IR-EX
23
0.2 %
PAR-IR-SEV
252
1.7 %
PAR-NEW-IN
4850
33.1 %
PAR-OTHER
776
5.3 %
PAR-PERSIS
1345
9.2 %
PAR-RAPID
136
0.9 %
PAR-RECUR
5992
40.9 %
SER-FLEXI
392
2.7 %
Total
14635
100.00 %
Figure 1. Number of samples submitted in each category during August 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
200
400
600
800
1000
1200
1400
1600
1800
Wigtown
Kirkcudbright
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Lancashire
Heref
Gloucs
E Sussex
Dorset
Devon
Cumbria
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during August 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
14000
16000
18000
Wigtown
West lothian
Kirkcudbright
Ayrshire
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Conwy
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
Humberside
Herts
Heref
Hampshire
Gloucs
E Sussex
Durham
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
1
59
0.40 %
2
3.39 %
55
93.22 %
2
3.39 %
2
3.39 %
0
0.00 %
PAR-RECUR
2
225
1.54 %
4
1.78 %
204
90.67 %
22
9.78 %
17
7.56 %
0
0.00 %
SER-FLEXI
3
53
0.36 %
0
0.00 %
51
96.23 %
2
3.77 %
2
3.77 %
0
0.00 %
Corn & Scilly
PAR-RECUR
12
1,640
11.21 %
53
3.23 %
1542
94.02 %
63
3.84 %
43
2.62 %
2
0.12 %
SER-FLEXI
2
46
0.31 %
8
17.39 %
37
80.43 %
4
8.70 %
1
2.17 %
0
0.00 %
Devon
PAR-OTHER
3
300
2.05 %
8
2.67 %
279
93.00 %
24
8.00 %
12
4.00 %
1
0.33 %
PAR-PERSIS
1
19
0.13 %
1
5.26 %
13
68.42 %
7
36.84 %
5
26.32 %
0
0.00 %
PAR-RECUR
3
374
2.56 %
12
3.21 %
339
90.64 %
27
7.22 %
21
5.61 %
2
0.53 %
Dorset
PAR-RECUR
1
125
0.85 %
4
3.20 %
113
90.40 %
8
6.40 %
8
6.40 %
0
0.00 %
Gloucs
PAR-RECUR
1
394
2.69 %
31
7.87 %
345
87.56 %
24
6.09 %
18
4.57 %
0
0.00 %
Heref
PAR-RECUR
3
240
1.64 %
3
1.25 %
225
93.75 %
27
11.25 %
11
4.58 %
1
0.42 %
Shropshire
PAR-OTHER
1
14
0.10 %
5
35.71 %
9
64.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
273
1.87 %
9
3.30 %
255
93.41 %
16
5.86 %
9
3.30 %
0
0.00 %
Somerset excl N
PAR-PERSIS
1
146
1.00 %
3
2.05 %
133
91.10 %
13
8.90 %
10
6.85 %
0
0.00 %
PAR-RECUR
3
148
1.01 %
4
2.70 %
140
94.59 %
7
4.73 %
4
2.70 %
0
0.00 %
Staffs
PAR-OTHER
2
67
0.46 %
7
10.45 %
54
80.60 %
8
11.94 %
5
7.46 %
1
1.49 %
PAR-RECUR
4
1,071
7.32 %
12
1.12 %
1021
95.33 %
51
4.76 %
37
3.45 %
1
0.09 %
Wiltshire
PAR-RECUR
2
409
2.79 %
33
8.07 %
367
89.73 %
21
5.13 %
7
1.71 %
2
0.49 %
England - Edge
Bucks
PAR-NEW-IN
2
30
0.20 %
0
0.00 %
28
93.33 %
2
6.67 %
2
6.67 %
0
0.00 %
PAR-RECUR
1
4
0.03 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Cheshire
PAR-CUL-P
1
291
1.99 %
33
11.34 %
253
86.94 %
15
5.15 %
5
1.72 %
0
0.00 %
PAR-PERSIS
2
9
0.06 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
228
1.56 %
12
5.26 %
211
92.54 %
5
2.19 %
5
2.19 %
0
0.00 %
SER-FLEXI
1
9
0.06 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
E Sussex
PAR-CUL-P
3
519
3.55 %
23
4.43 %
474
91.33 %
34
6.55 %
22
4.24 %
0
0.00 %
PAR-NEW-IN
4
211
1.44 %
9
4.27 %
192
91.00 %
10
4.74 %
10
4.74 %
0
0.00 %
Table 2a. Summary by County for August 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for August 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
2
597
4.08 %
27
4.52 %
554
92.80 %
21
3.52 %
15
2.51 %
1
0.17 %
Northants
PAR-NEW-IN
1
4
0.03 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
Oxon
PAR-NEW-IN
3
93
0.64 %
4
4.30 %
84
90.32 %
6
6.45 %
5
5.38 %
0
0.00 %
PAR-PERSIS
1
583
3.98 %
17
2.92 %
554
95.03 %
18
3.09 %
12
2.06 %
0
0.00 %
Warks
PAR-OTHER
1
40
0.27 %
1
2.50 %
36
90.00 %
4
10.00 %
3
7.50 %
0
0.00 %
PAR-RECUR
1
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
6
0.04 %
0
0.00 %
6
100.00 %
2
33.33 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
589
4.02 %
32
5.43 %
540
91.68 %
23
3.90 %
17
2.89 %
0
0.00 %
Lincs
PAR-NEW-IN
2
4
0.03 %
0
0.00 %
4
100.00 %
1
25.00 %
0
0.00 %
0
0.00 %
England
76
8825
60.30 %
357
4.05 %
8144
92.28 %
472
5.35 %
313
3.55 %
11
0.12 %
Wales - High TB
Carms
PAR-IR-SEV
4
17
0.12 %
6
35.29 %
10
58.82 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-PERSIS
1
2
0.01 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
6
17
0.12 %
9
52.94 %
8
47.06 %
1
5.88 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
139
0.95 %
14
10.07 %
119
85.61 %
12
8.63 %
6
4.32 %
0
0.00 %
Ceredigion
PAR-RAPID
2
20
0.14 %
6
30.00 %
14
70.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
37
0.25 %
3
8.11 %
33
89.19 %
1
2.70 %
1
2.70 %
0
0.00 %
Gwent
PAR-OTHER
1
15
0.10 %
3
20.00 %
12
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
31
0.21 %
9
29.03 %
22
70.97 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
70
0.48 %
2
2.86 %
65
92.86 %
3
4.29 %
3
4.29 %
0
0.00 %
Pembs
PAR-IR-EX
4
18
0.12 %
7
38.89 %
11
61.11 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
14
203
1.39 %
49
24.14 %
146
71.92 %
11
5.42 %
7
3.45 %
1
0.49 %
PAR-PERSIS
5
586
4.00 %
36
6.14 %
532
90.78 %
31
5.29 %
18
3.07 %
0
0.00 %
PAR-RAPID
9
41
0.28 %
10
24.39 %
31
75.61 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
8
482
3.29 %
8
1.66 %
452
93.78 %
36
7.47 %
22
4.56 %
0
0.00 %
SER-FLEXI
1
284
1.94 %
18
6.34 %
263
92.61 %
3
1.06 %
3
1.06 %
0
0.00 %
Powys
PAR-IR-EX
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
9
0.06 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for August 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
7
11
0.08 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
S Wales
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
3
338
2.31 %
10
2.96 %
321
94.97 %
10
2.96 %
7
2.07 %
0
0.00 %
PAR-RECUR
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
2
4
0.03 %
2
50.00 %
0
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
14
0.10 %
5
35.71 %
9
64.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
8
448
3.06 %
13
2.90 %
410
91.52 %
29
6.47 %
25
5.58 %
0
0.00 %
PAR-RAPID
3
5
0.03 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
126
0.86 %
1
0.79 %
120
95.24 %
5
3.97 %
5
3.97 %
0
0.00 %
Powys
PAR-RAPID
1
4
0.03 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
3
167
1.14 %
5
2.99 %
151
90.42 %
14
8.38 %
11
6.59 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
2
8
0.05 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
738
5.04 %
18
2.44 %
708
95.93 %
17
2.30 %
11
1.49 %
1
0.14 %
Wales
110
3847
26.29 %
245
6.37 %
3477
90.38 %
178
4.63 %
123
3.20 %
2
0.05 %
Scotland - Scotland
Kirkcudbright
PAR-NEW-IN
6
1,053
7.20 %
17
1.61 %
975
92.59 %
77
7.31 %
60
5.70 %
1
0.09 %
Wigtown
PAR-NEW-IN
5
910
6.22 %
20
2.20 %
853
93.74 %
60
6.59 %
37
4.07 %
0
0.00 %
Scotland
11
1963
13.41 %
37
1.88 %
1828
93.12 %
137
6.98 %
97
4.94 %
1
0.05 %
Grand Total
Sum:
197
14635
639
13449
787
533
14
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
4
392
0.26 %
49
12.50 %
319
81.38 %
26
6.63 %
24
6.12 %
0
0.00 %
PAR-PERSIS
5
756
0.51 %
91
12.04 %
621
82.14 %
48
6.35 %
44
5.82 %
0
0.00 %
PAR-RECUR
24
2,992
2.01 %
155
5.18 %
2659
88.87 %
218
7.29 %
176
5.88 %
2
0.07 %
SER-FLEXI
3
53
0.04 %
0
0.00 %
51
96.23 %
2
3.77 %
2
3.77 %
0
0.00 %
Corn & Scilly
PAR-CUL-P
2
891
0.60 %
35
3.93 %
805
90.35 %
58
6.51 %
51
5.72 %
0
0.00 %
PAR-OTHER
3
947
0.64 %
98
10.35 %
766
80.89 %
97
10.24 %
83
8.76 %
0
0.00 %
PAR-PERSIS
12
1,496
1.00 %
124
8.29 %
1223
81.75 %
169
11.30 %
148
9.89 %
1
0.07 %
PAR-RECUR
58
6,229
4.18 %
349
5.60 %
5299
85.07 %
548
8.80 %
474
7.61 %
107
1.72 %
SER-FLEXI
7
114
0.08 %
21
18.42 %
88
77.19 %
7
6.14 %
4
3.51 %
1
0.88 %
Devon
PAR-CHR
5
458
0.31 %
10
2.18 %
411
89.74 %
41
8.95 %
36
7.86 %
1
0.22 %
PAR-CUL-N
5
240
0.16 %
13
5.42 %
213
88.75 %
17
7.08 %
14
5.83 %
0
0.00 %
PAR-CUL-P
6
614
0.41 %
27
4.40 %
463
75.41 %
149
24.27 %
124
20.20 %
0
0.00 %
PAR-OTHER
9
616
0.41 %
18
2.92 %
542
87.99 %
71
11.53 %
55
8.93 %
1
0.16 %
PAR-PERSIS
13
1,793
1.20 %
92
5.13 %
1590
88.68 %
128
7.14 %
108
6.02 %
3
0.17 %
PAR-RECUR
62
10,184
6.84 %
429
4.21 %
9053
88.89 %
780
7.66 %
633
6.22 %
69
0.68 %
Dorset
PAR-CUL-P
1
6
0.00 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
588
0.39 %
29
4.93 %
552
93.88 %
16
2.72 %
7
1.19 %
0
0.00 %
PAR-RECUR
15
3,066
2.06 %
194
6.33 %
2744
89.50 %
155
5.06 %
126
4.11 %
2
0.07 %
SER-FLEXI
5
159
0.11 %
16
10.06 %
139
87.42 %
5
3.14 %
4
2.52 %
0
0.00 %
Gloucs
PAR-RECUR
26
4,008
2.69 %
181
4.52 %
3425
85.45 %
181
4.52 %
143
3.57 %
259
6.46 %
Heref
PAR-CUL-N
1
142
0.10 %
13
9.15 %
119
83.80 %
12
8.45 %
10
7.04 %
0
0.00 %
PAR-CUL-P
1
485
0.33 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
485
100.00 %
PAR-OTHER
1
150
0.10 %
10
6.67 %
131
87.33 %
11
7.33 %
9
6.00 %
0
0.00 %
PAR-PERSIS
3
565
0.38 %
25
4.42 %
525
92.92 %
18
3.19 %
15
2.65 %
0
0.00 %
PAR-RECUR
34
4,510
3.03 %
217
4.81 %
3948
87.54 %
403
8.94 %
343
7.61 %
2
0.04 %
Shropshire
PAR-CUL-P
3
370
0.25 %
39
10.54 %
320
86.49 %
11
2.97 %
11
2.97 %
0
0.00 %
Table 2b. Summary by County for 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
5
67
0.04 %
11
xx-xxxx-xxxx %
48
71.64 %
8
11.94 %
8
11.94 %
0
0.00 %
PAR-PERSIS
4
778
0.52 %
81
10.41 %
674
86.63 %
28
3.60 %
23
2.96 %
0
0.00 %
PAR-RECUR
42
7,418
4.98 %
259
3.49 %
6757
91.09 %
485
6.54 %
376
5.07 %
26
0.35 %
SER-FLEXI
4
430
0.29 %
87
20.23 %
307
71.40 %
44
10.23 %
36
8.37 %
0
0.00 %
Somerset excl N
PAR-CHR
2
78
0.05 %
4
5.13 %
73
93.59 %
2
2.56 %
1
1.28 %
0
0.00 %
PAR-CUL-P
8
990
0.66 %
43
4.34 %
880
88.89 %
65
6.57 %
56
5.66 %
11
1.11 %
PAR-OTHER
2
162
0.11 %
4
2.47 %
141
87.04 %
18
11.11 %
17
10.49 %
0
0.00 %
PAR-PERSIS
3
262
0.18 %
19
7.25 %
216
82.44 %
31
11.83 %
26
9.92 %
1
0.38 %
PAR-RECUR
29
3,780
2.54 %
199
5.26 %
3405
90.08 %
205
5.42 %
170
4.50 %
6
0.16 %
SER-FLEXI
1
56
0.04 %
3
5.36 %
52
92.86 %
2
3.57 %
1
1.79 %
0
0.00 %
Staffs
PAR-CHR
3
178
0.12 %
3
1.69 %
164
92.13 %
16
8.99 %
11
6.18 %
0
0.00 %
PAR-OTHER
2
67
0.04 %
7
10.45 %
54
80.60 %
8
11.94 %
5
7.46 %
1
1.49 %
PAR-PERSIS
2
233
0.16 %
53
22.75 %
162
69.53 %
27
11.59 %
18
7.73 %
0
0.00 %
PAR-RECUR
37
5,974
4.01 %
197
3.30 %
5479
91.71 %
358
5.99 %
292
4.89 %
6
0.10 %
Wiltshire
PAR-OTHER
1
470
0.32 %
13
2.77 %
442
94.04 %
23
4.89 %
15
3.19 %
0
0.00 %
PAR-RECUR
27
4,731
3.18 %
209
4.42 %
4332
91.57 %
220
4.65 %
168
3.55 %
22
0.47 %
England - Edge
Berks
PAR-NEW-IN
4
386
0.26 %
25
6.48 %
345
89.38 %
17
4.40 %
13
3.37 %
3
0.78 %
Bucks
PAR-NEW-IN
13
614
0.41 %
25
4.07 %
553
90.07 %
43
7.00 %
35
5.70 %
1
0.16 %
PAR-RECUR
5
337
0.23 %
20
5.93 %
296
87.83 %
25
7.42 %
21
6.23 %
0
0.00 %
Cheshire
PAR-CUL-P
5
1,152
0.77 %
44
3.82 %
1084
94.10 %
50
4.34 %
24
2.08 %
0
0.00 %
PAR-NEW-IN
2
1,061
0.71 %
32
3.02 %
792
74.65 %
287
27.05 %
237
22.34 %
0
0.00 %
PAR-OTHER
4
1,041
0.70 %
39
3.75 %
985
94.62 %
24
2.31 %
17
1.63 %
0
0.00 %
PAR-PERSIS
17
1,907
1.28 %
120
6.29 %
1581
82.91 %
232
12.17 %
206
10.80 %
0
0.00 %
PAR-RECUR
2
461
0.31 %
20
4.34 %
432
93.71 %
10
2.17 %
9
1.95 %
0
0.00 %
SER-FLEXI
8
1,397
0.94 %
47
3.36 %
1283
91.84 %
92
6.59 %
67
4.80 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
32
0.02 %
1
3.13 %
24
75.00 %
7
21.88 %
7
21.88 %
0
0.00 %
PAR-RECUR
10
1,470
0.99 %
36
2.45 %
1341
91.22 %
106
7.21 %
91
6.19 %
2
0.14 %
E Sussex
PAR-CUL-P
3
519
0.35 %
23
4.43 %
474
91.33 %
34
6.55 %
22
4.24 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
17
1,769
1.19 %
88
4.97 %
1580
89.32 %
122
6.90 %
100
5.65 %
1
0.06 %
Hampshire
PAR-NEW-IN
5
360
0.24 %
11
3.06 %
328
91.11 %
26
7.22 %
21
5.83 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
32
4,385
2.94 %
140
3.19 %
3925
89.51 %
386
8.80 %
318
7.25 %
2
0.05 %
Northants
PAR-NEW-IN
23
2,062
1.38 %
58
2.81 %
1849
89.67 %
145
7.03 %
128
6.21 %
27
1.31 %
PAR-RECUR
1
197
0.13 %
1
0.51 %
180
91.37 %
18
9.14 %
16
8.12 %
0
0.00 %
Notts
PAR-NEW-IN
7
499
0.33 %
18
3.61 %
425
85.17 %
59
11.82 %
56
11.22 %
0
0.00 %
Oxon
PAR-HERD-S
1
9
0.01 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-NEW-IN
10
747
0.50 %
34
4.55 %
637
85.27 %
89
11.91 %
75
10.04 %
1
0.13 %
PAR-PERSIS
2
784
0.53 %
32
4.08 %
728
92.86 %
40
5.10 %
24
3.06 %
0
0.00 %
PAR-RECUR
14
1,573
1.06 %
50
3.18 %
1442
91.67 %
93
5.91 %
77
4.90 %
4
0.25 %
Warks
PAR-OTHER
5
124
0.08 %
16
12.90 %
96
77.42 %
14
11.29 %
12
9.68 %
0
0.00 %
PAR-RECUR
8
901
0.60 %
46
5.11 %
760
84.35 %
83
9.21 %
95
10.54 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
2
33
0.02 %
2
6.06 %
9
27.27 %
5
15.15 %
5
15.15 %
17
51.52 %
Cumbria
PAR-NEW-IN
12
1,683
1.13 %
32
1.90 %
1563
92.87 %
100
5.94 %
87
5.17 %
1
0.06 %
SER-NSR
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-OTHER
1
7
0.00 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Durham
PAR-NEW-IN
4
109
0.07 %
3
2.75 %
95
87.16 %
8
7.34 %
7
6.42 %
4
3.67 %
Herts
PAR-NEW-IN
5
347
0.23 %
10
2.88 %
318
91.64 %
26
7.49 %
19
5.48 %
0
0.00 %
Humberside
PAR-NEW-IN
2
83
0.06 %
0
0.00 %
75
90.36 %
10
12.05 %
8
9.64 %
0
0.00 %
Kent
SER-OTHER
2
248
0.17 %
1
0.40 %
240
96.77 %
9
3.63 %
6
2.42 %
1
0.40 %
Lancashire
PAR-NEW-IN
5
692
0.46 %
33
4.77 %
638
92.20 %
28
4.05 %
20
2.89 %
1
0.14 %
Lincs
PAR-NEW-IN
11
537
0.36 %
14
2.61 %
502
93.48 %
31
5.77 %
21
3.91 %
0
0.00 %
PAR-PERSIS
2
131
0.09 %
5
3.82 %
123
93.89 %
8
6.11 %
3
2.29 %
0
0.00 %
SER-FLEXI
4
323
0.22 %
4
1.24 %
268
82.97 %
5
1.55 %
1
0.31 %
50
15.48 %
Norfolk
PAR-NEW-IN
4
112
0.08 %
7
6.25 %
75
66.96 %
27
24.11 %
27
24.11 %
3
2.68 %
N Yorks
PAR-NEW-IN
4
507
0.34 %
7
1.38 %
465
91.72 %
38
7.50 %
34
6.71 %
1
0.20 %
W Sussex
PAR-NEW-IN
3
704
0.47 %
32
4.55 %
660
93.75 %
16
2.27 %
11
1.56 %
1
0.14 %
SER-OTHER
2
13
0.01 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England
746
96818
64.98 %
4503
4.65 %
85400
88.21 %
7026
7.26 %
5789
5.98 %
1126
1.16 %
Wales - High TB
Carms
PAR-IR-EX
9
25
0.02 %
8
32.00 %
13
52.00 %
4
16.00 %
4
16.00 %
0
0.00 %
PAR-IR-SEV
46
334
0.22 %
115
34.43 %
206
61.68 %
14
4.19 %
11
3.29 %
2
0.60 %
PAR-NEW-IN
1
21
0.01 %
1
4.76 %
19
90.48 %
2
9.52 %
1
4.76 %
0
0.00 %
PAR-OTHER
5
232
0.16 %
20
8.62 %
198
85.34 %
20
8.62 %
14
6.03 %
0
0.00 %
PAR-PERSIS
24
3,590
2.41 %
222
6.18 %
3250
90.53 %
137
3.82 %
111
3.09 %
7
0.19 %
PAR-RAPID
41
166
0.11 %
67
40.36 %
96
57.83 %
7
4.22 %
3
1.81 %
0
0.00 %
PAR-RECUR
23
3,743
2.51 %
108
2.89 %
3530
94.31 %
155
4.14 %
103
2.75 %
2
0.05 %
SER-FLEXI
3
59
0.04 %
7
11.86 %
50
84.75 %
2
3.39 %
1
1.69 %
1
1.69 %
Ceredigion
PAR-HERD-S
1
35
0.02 %
6
17.14 %
27
77.14 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-IR-EX
6
29
0.02 %
16
55.17 %
13
44.83 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
11
79
0.05 %
37
46.84 %
42
53.16 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
5
486
0.33 %
30
6.17 %
419
86.21 %
42
8.64 %
36
7.41 %
1
0.21 %
PAR-RAPID
7
28
0.02 %
8
28.57 %
20
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
309
0.21 %
20
6.47 %
273
88.35 %
19
6.15 %
15
4.85 %
1
0.32 %
Gwent
PAR-IR-EX
3
19
0.01 %
8
42.11 %
9
47.37 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-IR-SEV
6
53
0.04 %
20
37.74 %
33
62.26 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
5
45
0.03 %
5
11.11 %
37
82.22 %
4
8.89 %
3
6.67 %
0
0.00 %
PAR-PERSIS
7
463
0.31 %
40
8.64 %
413
89.20 %
12
2.59 %
10
2.16 %
0
0.00 %
PAR-RAPID
16
88
0.06 %
39
44.32 %
48
54.55 %
2
2.27 %
1
1.14 %
0
0.00 %
PAR-RECUR
17
1,138
0.76 %
41
3.60 %
1054
92.62 %
49
4.31 %
43
3.78 %
0
0.00 %
SER-FLEXI
6
400
0.27 %
5
1.25 %
375
93.75 %
23
5.75 %
18
4.50 %
2
0.50 %
Pembs
PAR-IR-EX
24
96
0.06 %
41
42.71 %
54
56.25 %
1
1.04 %
1
1.04 %
0
0.00 %
PAR-IR-SEV
138
1,421
0.95 %
439
30.89 %
911
64.11 %
85
5.98 %
70
4.93 %
1
0.07 %
PAR-OTHER
9
551
0.37 %
66
11.98 %
468
84.94 %
26
4.72 %
17
3.09 %
0
0.00 %
PAR-PERSIS
69
8,225
5.52 %
509
6.19 %
7294
88.68 %
386
4.69 %
284
3.45 %
138
1.68 %
PAR-RAPID
62
205
0.14 %
62
30.24 %
134
65.37 %
10
4.88 %
9
4.39 %
0
0.00 %
PAR-RECUR
42
4,111
2.76 %
161
3.92 %
3737
90.90 %
268
6.52 %
204
4.96 %
9
0.22 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
17
1,796
1.21 %
107
5.96 %
1552
86.41 %
156
8.69 %
135
7.52 %
2
0.11 %
Powys
PAR-IR-EX
5
26
0.02 %
4
15.38 %
21
80.77 %
2
7.69 %
1
3.85 %
0
0.00 %
PAR-IR-SEV
30
224
0.15 %
71
31.70 %
146
65.18 %
7
3.13 %
7
3.13 %
0
0.00 %
PAR-OTHER
3
75
0.05 %
2
2.67 %
68
90.67 %
7
9.33 %
4
5.33 %
1
1.33 %
PAR-PERSIS
6
799
0.54 %
44
5.51 %
697
87.23 %
66
8.26 %
58
7.26 %
0
0.00 %
PAR-RAPID
17
28
0.02 %
8
28.57 %
20
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
18
1,155
0.78 %
64
5.54 %
1040
90.04 %
61
5.28 %
49
4.24 %
2
0.17 %
SER-FLEXI
2
177
0.12 %
26
14.69 %
98
55.37 %
60
33.90 %
53
29.94 %
0
0.00 %
S Wales
PAR-IR-SEV
3
23
0.02 %
6
26.09 %
16
69.57 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
28
0.02 %
4
14.29 %
22
78.57 %
1
3.57 %
1
3.57 %
1
3.57 %
Wales - Intermediate TB
Carms
PAR-OTHER
3
338
0.23 %
10
2.96 %
321
94.97 %
10
2.96 %
7
2.07 %
0
0.00 %
PAR-PERSIS
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-RECUR
2
63
0.04 %
4
6.35 %
57
90.48 %
2
3.17 %
2
3.17 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
21
0.01 %
17
80.95 %
4
19.05 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.01 %
4
44.44 %
2
22.22 %
2
22.22 %
2
22.22 %
1
11.11 %
Mid Glamorgan
PAR-IR-SEV
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
88
0.06 %
3
3.41 %
83
94.32 %
3
3.41 %
2
2.27 %
0
0.00 %
NE Wales
PAR-IR-EX
12
24
0.02 %
6
25.00 %
18
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
31
148
0.10 %
38
25.68 %
100
67.57 %
10
6.76 %
10
6.76 %
0
0.00 %
PAR-NEW-IN
53
4,229
2.84 %
131
3.10 %
3850
91.04 %
294
6.95 %
236
5.58 %
12
0.28 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
29
2,314
1.55 %
109
4.71 %
2115
91.40 %
101
4.36 %
81
3.50 %
9
0.39 %
PAR-RAPID
10
15
0.01 %
4
26.67 %
11
73.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
7
714
0.48 %
19
2.66 %
647
90.62 %
50
7.00 %
48
6.72 %
0
0.00 %
SER-FLEXI
7
743
0.50 %
35
4.71 %
678
91.25 %
39
5.25 %
30
4.04 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-IR-SEV
3
16
0.01 %
2
12.50 %
14
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
7
730
0.49 %
17
2.33 %
686
93.97 %
29
3.97 %
25
3.42 %
2
0.27 %
PAR-RAPID
3
22
0.01 %
5
22.73 %
14
63.64 %
3
13.64 %
3
13.64 %
0
0.00 %
PAR-RECUR
5
411
0.28 %
26
6.33 %
365
88.81 %
25
6.08 %
20
4.87 %
0
0.00 %
S Glamorgan
PAR-RAPID
4
16
0.01 %
5
31.25 %
11
68.75 %
1
6.25 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
75
0.05 %
24
32.00 %
49
65.33 %
2
2.67 %
2
2.67 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
1
28
0.02 %
0
0.00 %
28
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwynedd
PAR-IR-EX
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
12
0.01 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-NEW-IN
50
2,795
1.88 %
82
2.93 %
2481
88.77 %
269
9.62 %
228
8.16 %
4
0.14 %
PAR-RAPID
4
4
0.00 %
0
0.00 %
1
25.00 %
3
75.00 %
3
75.00 %
0
0.00 %
PAR-RECUR
1
18
0.01 %
0
0.00 %
18
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
10
0.01 %
3
30.00 %
6
60.00 %
1
10.00 %
1
10.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
35
0.02 %
13
37.14 %
21
60.00 %
1
2.86 %
1
2.86 %
0
0.00 %
PAR-NEW-IN
29
3,420
2.30 %
98
2.87 %
3055
89.33 %
299
8.74 %
261
7.63 %
6
0.18 %
PAR-PERSIS
2
59
0.04 %
5
8.47 %
53
89.83 %
1
1.69 %
1
1.69 %
0
0.00 %
PAR-RAPID
2
9
0.01 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RECUR
1
171
0.11 %
3
1.75 %
148
86.55 %
19
11.11 %
19
11.11 %
1
0.58 %
SER-FLEXI
3
162
0.11 %
30
18.52 %
104
64.20 %
31
19.14 %
28
17.28 %
0
0.00 %
Wales
997
46999
31.54 %
3144
6.69 %
41364
88.01 %
2832
6.03 %
2286
4.86 %
205
0.44 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
2
248
0.17 %
5
2.02 %
236
95.16 %
8
3.23 %
6
2.42 %
1
0.40 %
Kirkcudbright
PAR-NEW-IN
10
3,147
2.11 %
75
2.38 %
2945
93.58 %
168
5.34 %
125
3.97 %
2
0.06 %
West lothian
PAR-NEW-IN
1
27
0.02 %
0
0.00 %
16
59.26 %
11
40.74 %
11
40.74 %
0
0.00 %
Wigtown
PAR-NEW-IN
11
1,752
1.18 %
40
2.28 %
1591
90.81 %
156
8.90 %
119
6.79 %
2
0.11 %
Scotland
24
5174
3.47 %
120
2.32 %
4788
92.54 %
343
6.63 %
261
5.04 %
5
0.10 %
Grand Total
Sum:
1767 148991
7767
131552
10201
8336
1336
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol August 2024
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
70
8717
98.8%
349
4.0%
8049
92.3%
464
5.3%
308
3.5%
11
0.1%
Serial/Extended
6
108
1.2%
8
7.4%
95
88.0%
8
7.4%
5
4.6%
0
0.0 %
Total
76
8825
100.0%
357
4.0%
8144
92.3%
472
5.3%
313
3.5%
11
0.1%
Scotland
Parallel
11
1963
100.0%
37
1.9%
1828
93.1%
137
7.0%
97
4.9%
1
0.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
11
1963
100.0%
37
1.9%
1828
93.1%
137
7.0%
97
4.9%
1
0.1%
Wales
Parallel
109
3563
92.6%
227
6.4%
3214
90.2%
175
4.9%
120
3.4%
2
0.1%
Serial/Extended
1
284
7.4%
18
6.3%
263
92.6%
3
1.1%
3
1.1%
0
0.0 %
Total
110
3847
100.0%
245
6.4%
3477
90.4%
178
4.6%
123
3.2%
2
0.1%
GB
Parallel
190
14243
97.3%
613
4.3%
13091
91.9%
776
5.4%
525
3.7%
14
0.1%
Serial/Extended
7
392
2.7%
26
6.6%
358
91.3%
11
2.8%
8
2.0%
0
0.0 %
Total
197
14635
100.0%
639
4.4%
13449
91.9%
787
5.4%
533
3.6%
14
0.1%
76.00
110.00
11.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2024)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
708
94014
97.1%
4324
4.6%
82948
88.2%
6860
7.3%
5668
6.0%
1074
1.1%
Serial/Extended
38
2804
2.9%
179
6.4%
2452
87.4%
166
5.9%
121
4.3%
52
1.9%
Total
746
96818
100.0%
4503
4.7%
85400
88.2%
7026
7.3%
5789
6.0%
1126
1.2%
Scotland
Parallel
24
5174
100.0%
120
2.3%
4788
92.5%
343
6.6%
261
5.0%
5
0.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
24
5174
100.0%
120
2.3%
4788
92.5%
343
6.6%
261
5.0%
5
0.1%
Wales
Parallel
955
43577
92.7%
2907
6.7%
38452
88.2%
2518
5.8%
2018
4.6%
200
0.5%
Serial/Extended
42
3422
7.3%
237
6.9%
2912
85.1%
314
9.2%
268
7.8%
5
0.1%
Total
997
46999
100.0%
3144
6.7%
41364
88.0%
2832
6.0%
2286
4.9%
205
0.4%
GB
Parallel
xx-xxxx-xxxx 142765
95.8%
7351
5.1% 126188
88.4%
9721
6.8%
7947
5.6%
1279
0.9%
Serial/Extended
80
6226
4.2%
416
6.7%
5364
86.2%
480
7.7%
389
6.2%
57
0.9%
Total
1767 148991
100.0%
7767
5.2% 131552
88.3%
10201
6.8%
8336
5.6%
1336
0.9%
746.00
997.00
24.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
7
392
2.7%
26
6.6%
358
91.3%
11
2.8 %
8
2.0 %
0
Parallel – chronic breakdown
1
59
0.4%
2
3.4%
55
93.2%
2
3.4 %
2
3.4 %
0
Parallel - IRs in Exit Strategy for persistent her
7
23
0.2%
9
39.1%
14
60.9%
0
0
0
Parallel New Infection
44
4850
33.1%
145
3.0%
4507
92.9%
264
5.4 %
195
4.0 %
3
0.1 %
Parallel - Other
12
776
5.3%
34
4.4%
713
91.9%
46
5.9 %
27
3.5 %
2
0.3 %
Parallel Persistent Infection
11
1345
9.2%
58
4.3%
1242
92.3%
70
5.2 %
45
3.3 %
0
Parallel - persistent infection cull area
4
810
5.5%
56
6.9%
727
89.8%
49
6.0 %
27
3.3 %
0
Parallel - Rapid Testing Two-times IRs
35
136
0.9%
41
30.1%
92
67.6%
4
2.9 %
3
2.2 %
0
Parallel - Recurrent breakdowns
50
5992
40.9%
205
3.4%
5561
92.8%
329
5.5 %
218
3.6 %
8
0.1 %
Parallel - severe IRs - in persistent herds
26
252
1.7%
63
25.0%
180
71.4%
12
4.8 %
8
3.2 %
1
0.4 %
Total
197
14635
100.0%
639
4.4%
13449
91.9%
787
5.4 %
533
3.6 %
14
0.1 %
Table 4a. Summaryof IFN Gamma results by submission reason (August 2024)
England - HRA
Extended Flexible IFN-GAMMA test
5
99
0.7%
8
8.1%
88
88.9%
6
6.1 %
3
3.0 %
0
Parallel – chronic breakdown
1
59
0.4%
2
3.4%
55
93.2%
2
3.4 %
2
3.4 %
0
Parallel - Other
6
381
2.6%
20
5.2%
342
89.8%
32
8.4 %
17
4.5 %
2
0.5 %
Parallel Persistent Infection
2
165
1.1%
4
2.4%
146
88.5%
20
12.1 %
15
9.1 %
0
Parallel - Recurrent breakdowns
32
4899
33.5%
165
3.4%
4551
92.9%
266
5.4 %
175
3.6 %
8
0.2 %
England - HRA
46
5603
38.3%
199
3.6%
5182
92.5%
326
5.8 %
212
3.8 %
10
0.2 %
England - Edge
Extended Flexible IFN-GAMMA test
1
9
0.1%
0
0.0 %
7
77.8%
2
22.2 %
2
22.2 %
0
Parallel New Infection
12
935
6.4%
40
4.3%
860
92.0%
41
4.4 %
34
3.6 %
1
0.1 %
Parallel - Other
1
40
0.3%
1
2.5%
36
90.0%
4
10.0 %
3
7.5 %
0
Parallel Persistent Infection
3
592
4.0%
17
2.9%
563
95.1%
18
3.0 %
12
2.0 %
0
Parallel - persistent infection cull area
4
810
5.5%
56
6.9%
727
89.8%
49
6.0 %
27
3.3 %
0
Parallel - Recurrent breakdowns
3
237
1.6%
12
5.1%
219
92.4%
6
2.5 %
6
2.5 %
0
England - Edge
24
2623
17.9%
126
4.8%
2412
92.0%
120
4.6 %
84
3.2 %
1
0.0 %
England - LRA
Parallel New Infection
6
599
4.1%
32
5.3%
550
91.8%
26
4.3 %
17
2.8 %
0
England - LRA
6
599
4.1%
32
5.3%
550
91.8%
26
4.3 %
17
2.8 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
1
284
1.9%
18
6.3%
263
92.6%
3
1.1 %
3
1.1 %
0
Parallel - IRs in Exit Strategy for persistent her
5
21
0.1%
7
33.3%
14
66.7%
0
0
0
Parallel - Other
2
17
0.1%
3
17.6%
14
82.4%
0
0
0
Parallel Persistent Infection
6
588
4.0%
37
6.3%
533
90.6%
32
5.4 %
18
3.1 %
0
Parallel - Rapid Testing Two-times IRs
28
122
0.8%
36
29.5%
86
70.5%
1
0.8 %
0
0
Parallel - Recurrent breakdowns
13
728
5.0%
27
3.7%
669
91.9%
52
7.1 %
32
4.4 %
0
Parallel - severe IRs - in persistent herds
21
230
1.6%
57
24.8%
164
71.3%
12
5.2 %
8
3.5 %
1
0.4 %
Wales - High TB
76
1990
13.6%
185
9.3%
1743
87.6%
100
5.0 %
61
3.1 %
1
0.1 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
2
2
0.0%
2
100.0%
0
0.0 %
0
0
0
Parallel New Infection
8
448
3.1%
13
2.9%
410
91.5%
29
6.5 %
25
5.6 %
0
Parallel - Other
3
338
2.3%
10
3.0%
321
95.0%
10
3.0 %
7
2.1 %
0
Parallel - Rapid Testing Two-times IRs
6
13
0.1%
5
38.5%
6
46.2%
2
15.4 %
2
15.4 %
0
Parallel - Recurrent breakdowns
2
128
0.9%
1
0.8%
122
95.3%
5
3.9 %
5
3.9 %
0
Parallel - severe IRs - in persistent herds
3
14
0.1%
5
35.7%
9
64.3%
0
0
0
Wales - Intermediate TB
24
943
6.4%
36
3.8%
868
92.0%
46
4.9 %
39
4.1 %
0
Wales - Low TB
Parallel New Infection
7
905
6.2%
23
2.5%
859
94.9%
31
3.4 %
22
2.4 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel - severe IRs - in persistent herds
2
8
0.1%
1
12.5%
7
87.5%
0
0
0
Wales - Low TB
10
914
6.2%
24
2.6%
866
94.7%
32
3.5 %
23
2.5 %
1
0.1 %
Scotland - Scotland
Parallel New Infection
11
1963
13.4%
37
1.9%
1828
93.1%
137
7.0 %
97
4.9 %
1
0.1 %
Scotland - Scotland
11
1963
13.4%
37
1.9%
1828
93.1%
137
7.0 %
97
4.9 %
1
0.1 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
74
5954
4.0%
415
7.0%
5100
85.7%
471
7.9 %
383
6.4 %
56
0.9 %
Parallel – chronic breakdown
14
1106
0.7%
66
6.0%
967
87.4%
85
7.7 %
72
6.5 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
44
0.0%
6
13.6%
34
77.3%
4
9.1 %
4
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herds
62
244
0.2%
101
41.4%
135
55.3%
9
3.7 %
8
3.3 %
0
Parallel New Infection
333
33119
22.2%
1021
3.1%
29765
89.9%
2706
8.2 %
2241
6.8 %
92
0.3 %
Parallel - new infection cull area
6
382
0.3%
26
6.8%
332
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
58
4886
3.3%
319
6.5%
4298
88.0%
341
7.0 %
266
5.4 %
3
0.1 %
Parallel Persistent Infection
208
25231
16.9%
1631
6.5%
22237
88.1%
1490
5.9 %
1203
4.8 %
160
0.6 %
Parallel - persistent infection cull area
29
5027
3.4%
211
4.2%
4032
80.2%
367
7.3 %
288
5.7 %
496
9.9 %
Parallel - Rapid Testing Two-times IRs
174
596
0.4%
206
34.6%
365
61.2%
31
5.2 %
24
4.0 %
1
0.2 %
Parallel - Recurrent breakdowns
519
69780
46.8%
3015
4.3%
62526
89.6%
4540
6.5 %
3716
5.3 %
523
0.7 %
Parallel - severe IRs - in persistent herds
282
2350
1.6%
749
31.9%
1497
63.7%
119
5.1 %
101
4.3 %
3
0.1 %
Serial - Other
5
268
0.2%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Total
1767 148991
100.0%
7767
5.2%
131552
88.3%
10201
6.8 %
8336
5.6 %
1336
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2024)
England - HRA
Extended Flexible IFN-GAMMA test
20
812
0.5%
127
15.6%
637
78.4%
60
7.4 %
47
5.8 %
1
0.1 %
Parallel – chronic breakdown
14
1106
0.7%
66
6.0%
967
87.4%
85
7.7 %
72
6.5 %
1
0.1 %
Parallel - new infection cull area
6
382
0.3%
26
6.8%
332
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
23
2479
1.7%
161
6.5%
2124
85.7%
236
9.5 %
192
7.7 %
2
0.1 %
Parallel Persistent Infection
43
6471
4.3%
514
7.9%
5563
86.0%
465
7.2 %
389
6.0 %
5
0.1 %
Parallel - persistent infection cull area
21
3356
2.3%
144
4.3%
2474
73.7%
283
8.4 %
242
7.2 %
496
14.8 %
Parallel - Recurrent breakdowns
354
52892
35.5%
2389
4.5%
47101
89.1%
3553
6.7 %
2901
5.5 %
501
0.9 %
England - Edge
Extended Flexible IFN-GAMMA test
8
1397
0.9%
47
3.4%
1283
91.8%
92
6.6 %
67
4.8 %
0
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
7
77.8%
2
22.2 %
2
22.2 %
0
Parallel New Infection
116
11915
8.0%
432
3.6%
10458
87.8%
1181
9.9 %
990
8.3 %
35
0.3 %
Parallel - Other
9
1165
0.8%
55
4.7%
1081
92.8%
38
3.3 %
29
2.5 %
0
Parallel Persistent Infection
19
2691
1.8%
152
5.6%
2309
85.8%
272
10.1 %
230
8.5 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2024) Cont.
Parallel - persistent infection cull area
8
1671
1.1%
67
4.0%
1558
93.2%
84
5.0 %
46
2.8 %
0
Parallel - Recurrent breakdowns
40
4939
3.3%
173
3.5%
4451
90.1%
335
6.8 %
309
6.3 %
6
0.1 %
England - LRA
Extended Flexible IFN-GAMMA test
4
323
0.2%
4
1.2%
268
83.0%
5
1.5 %
1
0.3 %
50
15.5 %
Parallel New Infection
52
4807
3.2%
140
2.9%
4400
91.5%
289
6.0 %
239
5.0 %
28
0.6 %
Parallel Persistent Infection
2
131
0.1%
5
3.8%
123
93.9%
8
6.1 %
3
2.3 %
0
Serial - Other
5
268
0.2%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
4
100.0%
0
0
0
England
746
96818
65.0%
4503
4.7%
85400
88.2%
7026
7.3 %
5789
6.0 %
1126
1.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
28
2432
1.6%
145
6.0%
2075
85.3%
241
9.9 %
207
8.5 %
5
0.2 %
Parallel - Herd or Group Slaughter
1
35
0.0%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent herd
47
195
0.1%
77
39.5%
110
56.4%
9
4.6 %
8
4.1 %
0
Parallel New Infection
1
21
0.0%
1
4.8%
19
90.5%
2
9.5 %
1
4.8 %
0
Parallel - Other
22
903
0.6%
93
10.3%
771
85.4%
57
6.3 %
38
4.2 %
1
0.1 %
Parallel Persistent Infection
111
13563
9.1%
845
6.2%
12073
89.0%
643
4.7 %
499
3.7 %
146
1.1 %
Parallel - Rapid Testing Two-times IRs
145
518
0.3%
184
35.5%
321
62.0%
19
3.7 %
13
2.5 %
0
Parallel - Recurrent breakdowns
107
10484
7.0%
398
3.8%
9656
92.1%
553
5.3 %
415
4.0 %
15
0.1 %
Parallel - severe IRs - in persistent herds
234
2134
1.4%
688
32.2%
1354
63.4%
107
5.0 %
89
4.2 %
3
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
8
818
0.5%
59
7.2%
727
88.9%
41
5.0 %
32
3.9 %
0
Parallel - IRs in Exit Strategy for persistent herd
13
45
0.0%
23
51.1%
22
48.9%
0
0
0
Parallel New Infection
60
4959
3.3%
148
3.0%
4536
91.5%
323
6.5 %
261
5.3 %
14
0.3 %
Parallel - Other
4
339
0.2%
10
2.9%
322
95.0%
10
2.9 %
7
2.1 %
0
Parallel Persistent Infection
31
2316
1.6%
110
4.7%
2116
91.4%
101
4.4 %
81
3.5 %
9
0.4 %
Parallel - Rapid Testing Two-times IRs
23
65
0.0%
19
29.2%
39
60.0%
7
10.8 %
6
9.2 %
1
1.5 %
Parallel - Recurrent breakdowns
16
1276
0.9%
52
4.1%
1152
90.3%
80
6.3 %
72
5.6 %
0
Parallel - severe IRs - in persistent herds
38
169
0.1%
42
24.9%
117
69.2%
10
5.9 %
10
5.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
6
172
0.1%
33
19.2%
110
64.0%
32
18.6 %
29
16.9 %
0
Parallel - IRs in Exit Strategy for persistent herd
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
80
6243
4.2%
180
2.9%
5564
89.1%
568
9.1 %
489
7.8 %
10
0.2 %
Parallel Persistent Infection
2
59
0.0%
5
8.5%
53
89.8%
1
1.7 %
1
1.7 %
0
Parallel - Rapid Testing Two-times IRs
6
13
0.0%
3
23.1%
5
38.5%
5
38.5 %
5
38.5 %
0
Parallel - Recurrent breakdowns
2
189
0.1%
3
1.6%
166
87.8%
19
10.1 %
19
10.1 %
1
0.5 %
Parallel - severe IRs - in persistent herds
10
47
0.0%
19
40.4%
26
55.3%
2
4.3 %
2
4.3 %
0
Wales
997
46999
31.5%
3144
6.7%
41364
88.0%
2832
6.0 %
2286
4.9 %
205
0.4 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2024) Cont.
Scotland - Scotland
Parallel New Infection
24
5174
3.5%
120
2.3%
4788
92.5%
343
6.6 %
261
5.0 %
5
0.1 %
Scotland
24
5174
3.5%
120
2.3%
4788
92.5%
343
6.6 %
261
5.0 %
5
0.1 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2023 September
221
18070
112
638
10
0
0
0
0
52
2
0
12
0
503
0
56
1
0
October
253
18656
139
825
1
7
0
0
0
0
50
2
0
9
0
701
0
53
0
2
November
250
22290
157
1277
6
24
0
0
0
0
142
8
0
18
0
982
0
97
0
0
December
183
14037
114
892
5
0
0
0
0
69
3
0
18
0
753
0
44
0
0
2024 January
234
21995
171
1438
1
26
0
0
0
0
54
3
1
6
0
1254
1
91
0
1
February
257
20510
159
1189
13
0
0
0
0
31
1
0
12
0
1021
1
109
1
0
March
215
19876
142
1054
15
0
0
0
0
70
2
0
26
0
879
0
60
0
0
April
237
18969
146
1092
4
0
0
0
0
44
4
0
11
0
958
0
71
0
0
May
207
15411
129
733
1
0
0
0
0
11
3
0
9
0
656
0
53
0
0
June
197
18101
133
797
9
0
0
0
0
32
0
0
19
1
720
14
2
0
0
July
223
19494
124
825
14
246
0
0
0
0
23
0
4
8
2
516
5
5
1
0
August
197
14635
123
639
348
80
0
0
0
0
0
0
0
0
0
1
0
0
0
0
Totals (last
12 months)
2674
222044
xx-xxxx-xxxx
11399
370
440
0
0
0
0
578
28
5
148
3
8944
21
641
3
3
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1101
145078
740
6722
276
0
0
0
0
554
14
1
82
0
5387
1
82
1
0
Scotland
52
8891
35
195
47
0
0
0
0
0
1
0
2
0
61
0
33
1
0
Wales
1521
68075
874
4482
117
0
0
0
0
24
13
4
64
3
3496
20
526
1
3
Totals
2674
222044
xx-xxxx-xxxx
11399
440
0
0
0
0
578
28
5
148
3
8944
21
641
3
3
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
101
108
112
140
109
112
114
139
93
157
69
114
63
171
98
159
73
142
91
146
78
129
64
133
99
124
74
123
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2022
2023
2024
IFNg Positive herds
IFNg Negative herds
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
19561
867
17206
638
17659
825
20756
1277
13035
892
20308
1434
18508
1189
18373
1054
17402
1092
14468
733
17182
797
18485
825
13771
639
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2022
2023
2024
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
10
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2022
2023
2024
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
Wiltshire
Wigtown
Warks
S Wales
Staffs
Somerset excl N
Shropshire
Powys
Pembs
Oxon
Northants
NE Wales
Lincs
Leics & Rut
Lancashire
Kirkcudbright
Heref
Gwynedd
Gwent
Gloucs
E Sussex
Dorset
Devon
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during August 2024 (% submission per
County)
County
%Resampled %Retested
Avon
6.23 %
7.72 %
Bucks
8.82 %
8.82 %
Carms
2.72 %
4.85 %
Ceredigion
4.92 %
4.92 %
Cheshire
2.23 %
4.10 %
Corn & Scilly
2.61 %
3.97 %
Cumbria
33.33 %
Devon
5.48 %
8.37 %
Dorset
6.40 %
6.40 %
E Sussex
4.38 %
6.03 %
Gloucs
4.57 %
6.09 %
Gwent
2.59 %
2.59 %
Gwynedd
7.14 %
8.93 %
Heref
4.58 %
11.25 %
Kirkcudbright
5.70 %
7.31 %
Lancashire
2.89 %
3.90 %
Leics & Rut
2.51 %
3.52 %
Lincs
25.00 %
NE Wales
3.06 %
3.80 %
Northants
50.00 %
50.00 %
Oxon
2.51 %
3.55 %
Pembs
3.10 %
5.02 %
Powys
Shropshire
3.14 %
5.57 %
Somerset excl
4.76 %
6.80 %
Staffs
3.69 %
5.18 %
S Wales
Warks
6.67 %
8.89 %
Wigtown
4.07 %
6.59 %
Wiltshire
1.71 %
5.13 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
8737
357
4.1%
8825
357
4.0%
Wales
3778
245
6.5%
3847
245
6.4%
Scotland
1895
37
2.0%
1963
37
1.9%
GB
14410
639
4.4%
14635
639
4.4%
Table 6a (i): Number of animals and submissions tested in August 2024
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
90855
4499
5.0%
96818
4503
4.7%
Wales
44489
3142
7.1%
46999
3144
6.7%
Scotland
4971
120
2.4%
5174
120
2.3%
GB
140312
7761
5.5%
148991
7767
5.2%
Table 6b (i): Number of animals and submissions tested in 2024 Up to end of August
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
14598
598
4.1%
14712
598
4.1%
Wales
5248
261
5.0%
5366
261
4.9%
Scotland
582
8
1.4%
603
8
1.3%
GB
20428
867
4.2%
20681
867
4.2%
Table 6a (ii): Number of animals and submissions tested in August 2023
Table 6b (ii): Number of animals and submissions tested in 2023 Up to end of August
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
90815
3821
4.2%
95998
3822
4.0%
Wales
40078
2176
5.4%
42725
2176
5.1%
Scotland
4981
395
7.9%
8065
395
4.9%
GB
135856
6392
4.7%
146788
6393
4.4%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Worcestershire
PRV-SP
1
1
0
0
Total
1
1
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples August 2024
Table 7b Number of private samples 2024
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SE
2
19
0
0
PRV-SP
2
4
0
0
Devon
PRV-SE
4
137
9
6.57 %
Dorset
PRV-SP
1
1
0
0
East Sussex
PRV-SP
1
1
0
0
Gloucestershire
PRV-SE
1
78
7
8.97 %
North Yorkshire
PRV-SP
1
1
0
0
Oxfordshire
PRV-SE
1
5
0
0
Shropshire
PRV-SP
1
4
0
0
Wiltshire
PRV-SP
1
1
0
0
Worcestershire
PRV-SE
1
1
0
0
PRV-SP
1
1
0
0
Total
17
253
16
6.32 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Northern Somerset &
South
Gloucestershire
BOV-P
1
33
1
3.0%
England
1
33
1
3.0%
Wales
Carmarthenshire
BOV-IR-C
2
11
5
45.5%
Ceredigion
BOV-IR-C
2
54
12
22.2%
North East Wales
BOV-IR-C
3
15
5
33.3%
BOV-IR-EX
1
1
1
100.0%
Pembrokeshire
BOV-IR-C
15
211
45
21.3%
BOV-IR-EX
2
9
6
66.7%
BOV-P
1
14
1
7.1%
Powys
BOV-IR-C
2
9
2
22.2%
Wales
28
324
77
23.8%
All GB
29
357
78
21.8 %
Table 8a Number of Cattle Antibody Tests August 2024
Table 8b Number of Cattle Antibody Tests 2024
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cheshire
BOV-P
1
7
1
14.3%
Cumbria
BOV-OTHER
4
885
22
2.5%
Devon
BOV-OTHER
3
422
27
6.4%
Northern Somerset &
South
Gloucestershire
BOV-P
1
33
1
3.0%
Oxfordshire
BOV-P
1
114
10
8.8%
England
10
1,461
61
4.2%
Wales
Carmarthenshire
BOV-IR-C
32
325
58
17.8%
BOV-IR-EX
6
21
9
42.9%
BOV-OTHER
1
7
0
0
BOV-P
1
57
0
0
Ceredigion
BOV-IR-C
18
132
29
22.0%
BOV-IR-EX
7
50
15
30.0%
North East Wales
BOV-IR-C
22
146
30
20.5%
BOV-IR-EX
5
14
3
21.4%
BOV-OTHER
3
110
9
8.2%
BOV-P
3
111
7
6.3%
North West Wales
BOV-IR-C
1
5
0
0
Pembrokeshire
BOV-IR-C
131
1,490
301
20.2%
BOV-IR-EX
21
105
38
36.2%
BOV-OTHER
3
120
4
3.3%
BOV-P
4
243
21
8.6%
Powys
BOV-IR-C
29
281
40
14.2%
BOV-IR-EX
3
20
1
5.0%
South Wales
BOV-IR-C
11
88
19
21.6%
BOV-IR-EX
1
16
4
25.0%
BOV-P
2
384
10
2.6%
BOV-POSTM
1
75
19
25.3%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Wales
305
3,800
617
16.2%
All GB
315
5,261
678
12.9 %