Monthly report: Surveillance Project SB4008 IFNg tests for Bovine tuberculosis - January 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number
Report Period
Issued 10 February 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
January
2019
%
Total 2019
%
Submissions
289
289
Samples
Total (%)
27513
100.00 %
27513
100.00 %
England
20585
74.82 %
20585
74.82 %
Wales
6928
25.18 %
6928
25.18 %
Scotland
Parallel Tests
PAR-CUL-N
2848
10.35 %
2848
10.35 %
PAR-CUL-P
2527
9.18 %
2527
9.18 %
PAR-HERD-S
634
2.30 %
634
2.30 %
PAR-NEW-IN
13956
50.73 %
13956
50.73 %
PAR-OTHER
1605
5.83 %
1605
5.83 %
PAR-PERSIS
5313
19.31 %
5313
19.31 %
PAR-RAPID
173
0.63 %
173
0.63 %
Total(% of all samples)
27056
98.34 %
27056
98.34 %
Serial/Extended Tests
SER-FLEXI
450
1.64 %
450
1.64 %
SER-FRAUD
0
0.00 %
SER-NSR
7
0.03 %
7
0.03 %
SER-OTHER
0
0.00 %
Total(% of all samples)
457
1.66 %
457
1.66 %
Total
27513
100.00 %
27513
100.00 %
Retests
Total (% of all samples)
1937
7.04 %
1937
7.04 %
England
1526
5.55 %
1526
5.55 %
Wales
411
1.49 %
411
1.49 %
Scotland
Resamples
Total (% of all samples)
1657
6.02 %
1657
6.02 %
England
1335
4.85 %
1335
4.85 %
Wales
322
1.17 %
322
1.17 %
Scotland
Rejects
Total (% of all samples)
974
3.54 %
974
3.54 %
England
860
3.13 %
860
3.13 %
Wales
114
0.41 %
114
0.41 %
Scotland
0
0.00 %
Table 1. Monthly Totals for January 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CUL-N
2848
10.4 %
PAR-CUL-P
2527
9.2 %
PAR-HERD-S
634
2.3 %
PAR-NEW-IN
13956
50.7 %
PAR-OTHER
1605
5.8 %
PAR-PERSIS
5313
19.3 %
PAR-RAPID
173
0.6 %
SER-FLEXI
450
1.6 %
SER-NSR
7
0.0 %
Total
27513
100.00 %
Figure 1. Number of samples submitted in each category during January 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
W Glamorgan
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
S Yorks
Somerset
Shropshire
Oxon
Notts
Northants
Lincs
Leics & Rut
Lancashire
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Wales
England
SER-NSR
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during January 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
W Glamorgan
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
S Yorks
Somerset
Shropshire
Oxon
Notts
Northants
Lincs
Leics & Rut
Lancashire
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Wales
England
SER-NSR
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Table 2a. Summary by County for January 2019
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
2
200
0.73 %
12
6.00 %
169
84.50 %
20
10.00 %
17
8.50 %
2
1.00 %
Corn & Scilly
PAR-CUL-N
7
697
2.53 %
27
3.87 %
638
91.54 %
41
5.88 %
32
4.59 %
0
0.00 %
PAR-CUL-P
2
989
3.59 %
17
1.72 %
873
88.27 %
119
12.03 %
97
9.81 %
2
0.20 %
PAR-PERSIS
2
106
0.39 %
1
0.94 %
9
8.49 %
1
0.94 %
1
0.94 %
95
89.62 %
SER-FLEXI
2
15
0.05 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
8
810
2.94 %
65
8.02 %
694
85.68 %
59
7.28 %
50
6.17 %
1
0.12 %
PAR-CUL-P
2
441
1.60 %
18
4.08 %
401
90.93 %
29
6.58 %
22
4.99 %
0
0.00 %
PAR-NEW-IN
1
344
1.25 %
67
19.48 %
242
70.35 %
42
12.21 %
35
10.17 %
0
0.00 %
PAR-PERSIS
3
396
1.44 %
25
6.31 %
330
83.33 %
42
10.61 %
41
10.35 %
0
0.00 %
SER-FLEXI
2
62
0.23 %
6
9.68 %
25
40.32 %
0
0.00 %
0
0.00 %
31
50.00 %
Dorset
PAR-CUL-N
5
378
1.37 %
23
6.08 %
314
83.07 %
41
10.85 %
40
10.58 %
1
0.26 %
PAR-CUL-P
2
869
3.16 %
38
4.37 %
795
91.48 %
37
4.26 %
36
4.14 %
0
0.00 %
Gloucs
PAR-CUL-N
6
620
2.25 %
30
4.84 %
446
71.94 %
53
8.55 %
44
7.10 %
100
16.13 %
Heref
PAR-CUL-N
2
343
1.25 %
15
4.37 %
311
90.67 %
18
5.25 %
17
4.96 %
0
0.00 %
Shropshire
PAR-OTHER
2
454
1.65 %
24
5.29 %
392
86.34 %
44
9.69 %
38
8.37 %
0
0.00 %
PAR-PERSIS
1
72
0.26 %
2
2.78 %
56
77.78 %
15
20.83 %
14
19.44 %
0
0.00 %
Somerset
PAR-CUL-P
1
228
0.83 %
21
9.21 %
196
85.96 %
17
7.46 %
9
3.95 %
2
0.88 %
PAR-OTHER
2
238
0.87 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
Wiltshire
PAR-OTHER
1
36
0.13 %
1
2.78 %
33
91.67 %
4
11.11 %
2
5.56 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
7
1,009
3.67 %
23
2.28 %
921
91.28 %
72
7.14 %
65
6.44 %
0
0.00 %
PAR-PERSIS
1
92
0.33 %
4
4.35 %
87
94.57 %
2
2.17 %
1
1.09 %
0
0.00 %
Bucks
PAR-NEW-IN
1
153
0.56 %
12
7.84 %
138
90.20 %
7
4.58 %
3
1.96 %
0
0.00 %
Cheshire
PAR-NEW-IN
23
4,679
17.01 %
108
2.31 %
4172
89.16 %
294
6.28 %
249
5.32 %
150
3.21 %
PAR-PERSIS
1
13
0.05 %
1
7.69 %
12
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
18
2,797
10.17 %
85
3.04 %
2365
84.55 %
154
5.51 %
134
4.79 %
213
7.62 %
E Sussex
PAR-NEW-IN
5
807
2.93 %
46
5.70 %
719
89.10 %
30
3.72 %
29
3.59 %
13
1.61 %
Hampshire
PAR-NEW-IN
6
55
0.20 %
1
1.82 %
51
92.73 %
3
5.45 %
3
5.45 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-NSR
1
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
5
475
1.73 %
22
4.63 %
316
66.53 %
54
11.37 %
50
10.53 %
87
18.32 %
Northants
PAR-NEW-IN
1
6
0.02 %
0
0.00 %
5
83.33 %
2
33.33 %
1
16.67 %
0
0.00 %
Notts
PAR-NEW-IN
3
377
1.37 %
15
3.98 %
259
68.70 %
44
11.67 %
40
10.61 %
63
16.71 %
Oxon
PAR-NEW-IN
17
1,858
6.75 %
101
5.44 %
1570
84.50 %
198
10.66 %
187
10.06 %
0
0.00 %
Warks
PAR-NEW-IN
10
546
1.98 %
24
4.40 %
428
78.39 %
38
6.96 %
35
6.41 %
59
10.81 %
PAR-PERSIS
2
157
0.57 %
9
5.73 %
137
87.26 %
12
7.64 %
11
7.01 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
1
7
0.03 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
Cumbria
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
22
0.08 %
0
0.00 %
21
95.45 %
1
4.55 %
1
4.55 %
0
0.00 %
Lincs
PAR-NEW-IN
3
60
0.22 %
8
13.33 %
41
68.33 %
11
18.33 %
11
18.33 %
0
0.00 %
S Yorks
PAR-NEW-IN
1
162
0.59 %
4
2.47 %
150
92.59 %
10
6.17 %
8
4.94 %
0
0.00 %
England
162
20585
74.82 %
889
4.32 %
17501
85.02 %
1526
7.41 %
1335
6.49 %
860
4.18 %
Wales - High TB
Carms
PAR-HERD-S
5
455
1.65 %
38
8.35 %
401
88.13 %
24
5.27 %
15
3.30 %
1
0.22 %
PAR-PERSIS
17
2,952
10.73 %
133
4.51 %
2702
91.53 %
139
4.71 %
104
3.52 %
13
0.44 %
PAR-RAPID
11
63
0.23 %
25
39.68 %
34
53.97 %
8
12.70 %
4
6.35 %
0
0.00 %
SER-FLEXI
4
105
0.38 %
6
5.71 %
93
88.57 %
6
5.71 %
6
5.71 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
310
1.13 %
17
5.48 %
279
90.00 %
17
5.48 %
14
4.52 %
0
0.00 %
PAR-RAPID
4
5
0.02 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
3
392
1.42 %
18
4.59 %
347
88.52 %
31
7.91 %
26
6.63 %
1
0.26 %
PAR-RAPID
8
36
0.13 %
10
27.78 %
22
61.11 %
4
11.11 %
3
8.33 %
1
2.78 %
Pembs
PAR-HERD-S
3
49
0.18 %
3
6.12 %
45
91.84 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-OTHER
5
857
3.11 %
30
3.50 %
767
89.50 %
70
8.17 %
60
7.00 %
0
0.00 %
PAR-PERSIS
6
397
1.44 %
18
4.53 %
357
89.92 %
27
6.80 %
21
5.29 %
1
0.25 %
PAR-RAPID
13
33
0.12 %
7
21.21 %
25
75.76 %
2
6.06 %
1
3.03 %
0
0.00 %
SER-FLEXI
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
3
3
0.01 %
0
0.00 %
2
66.67 %
0
0.00 %
0
0.00 %
1
33.33 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
2
7
0.03 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-PERSIS
2
213
0.77 %
31
14.55 %
172
80.75 %
14
6.57 %
10
4.69 %
0
0.00 %
PAR-RAPID
4
34
0.12 %
4
11.76 %
11
32.35 %
2
5.88 %
2
5.88 %
17
50.00 %
SER-FLEXI
1
14
0.05 %
1
7.14 %
13
92.86 %
0
0.00 %
0
0.00 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
117
0.43 %
7
5.98 %
108
92.31 %
5
4.27 %
2
1.71 %
0
0.00 %
PAR-PERSIS
1
7
0.03 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
20
0.07 %
2
10.00 %
12
60.00 %
6
30.00 %
6
30.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
515
1.87 %
18
3.50 %
456
88.54 %
37
7.18 %
31
6.02 %
10
1.94 %
PAR-PERSIS
1
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
8
248
0.90 %
19
7.66 %
166
66.94 %
10
4.03 %
10
4.03 %
53
21.37 %
W Glamorgan
PAR-HERD-S
1
6
0.02 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
28
0.10 %
0
0.00 %
26
92.86 %
1
3.57 %
1
3.57 %
1
3.57 %
NE Wales
PAR-NEW-IN
4
46
0.17 %
0
0.00 %
31
67.39 %
1
2.17 %
0
0.00 %
15
32.61 %
Wales
127
6928
25.18 %
394
5.69 %
6098
88.02 %
411
5.93 %
322
4.65 %
114
1.65 %
Grand Total
Sum:
289
27513
1283
23599
1937
1657
974
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
2
200
0.73 %
12
6.00 %
169
84.50 %
20
10.00 %
17
8.50 %
2
1.00 %
Corn & Scilly
PAR-CUL-N
7
697
2.53 %
27
3.87 %
638
91.54 %
41
5.88 %
32
4.59 %
0
0.00 %
PAR-CUL-P
2
989
3.59 %
17
1.72 %
873
88.27 %
119
12.03 %
97
9.81 %
2
0.20 %
PAR-PERSIS
2
106
0.39 %
1
0.94 %
9
8.49 %
1
0.94 %
1
0.94 %
95
89.62 %
SER-FLEXI
2
15
0.05 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
8
810
2.94 %
65
8.02 %
694
85.68 %
59
7.28 %
50
6.17 %
1
0.12 %
PAR-CUL-P
2
441
1.60 %
18
4.08 %
401
90.93 %
29
6.58 %
22
4.99 %
0
0.00 %
PAR-NEW-IN
1
344
1.25 %
67
19.48 %
242
70.35 %
42
12.21 %
35
10.17 %
0
0.00 %
PAR-PERSIS
3
396
1.44 %
25
6.31 %
330
83.33 %
42
10.61 %
41
10.35 %
0
0.00 %
SER-FLEXI
2
62
0.23 %
6
9.68 %
25
40.32 %
0
0.00 %
0
0.00 %
31
50.00 %
Dorset
PAR-CUL-N
5
378
1.37 %
23
6.08 %
314
83.07 %
41
10.85 %
40
10.58 %
1
0.26 %
PAR-CUL-P
2
869
3.16 %
38
4.37 %
795
91.48 %
37
4.26 %
36
4.14 %
0
0.00 %
Gloucs
PAR-CUL-N
6
620
2.25 %
30
4.84 %
446
71.94 %
53
8.55 %
44
7.10 %
100
16.13 %
Heref
PAR-CUL-N
2
343
1.25 %
15
4.37 %
311
90.67 %
18
5.25 %
17
4.96 %
0
0.00 %
Shropshire
PAR-OTHER
2
454
1.65 %
24
5.29 %
392
86.34 %
44
9.69 %
38
8.37 %
0
0.00 %
PAR-PERSIS
1
72
0.26 %
2
2.78 %
56
77.78 %
15
20.83 %
14
19.44 %
0
0.00 %
Somerset
PAR-CUL-P
1
228
0.83 %
21
9.21 %
196
85.96 %
17
7.46 %
9
3.95 %
2
0.88 %
PAR-OTHER
2
238
0.87 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
Wiltshire
PAR-OTHER
1
36
0.13 %
1
2.78 %
33
91.67 %
4
11.11 %
2
5.56 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
7
1,009
3.67 %
23
2.28 %
921
91.28 %
72
7.14 %
65
6.44 %
0
0.00 %
PAR-PERSIS
1
92
0.33 %
4
4.35 %
87
94.57 %
2
2.17 %
1
1.09 %
0
0.00 %
Bucks
PAR-NEW-IN
1
153
0.56 %
12
7.84 %
138
90.20 %
7
4.58 %
3
1.96 %
0
0.00 %
Cheshire
PAR-NEW-IN
23
4,679
17.01 %
108
2.31 %
4172
89.16 %
294
6.28 %
249
5.32 %
150
3.21 %
PAR-PERSIS
1
13
0.05 %
1
7.69 %
12
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
18
2,797
10.17 %
85
3.04 %
2365
84.55 %
154
5.51 %
134
4.79 %
213
7.62 %
E Sussex
PAR-NEW-IN
5
807
2.93 %
46
5.70 %
719
89.10 %
30
3.72 %
29
3.59 %
13
1.61 %
Hampshire
PAR-NEW-IN
6
55
0.20 %
1
1.82 %
51
92.73 %
3
5.45 %
3
5.45 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-NSR
1
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
5
475
1.73 %
22
4.63 %
316
66.53 %
54
11.37 %
50
10.53 %
87
18.32 %
Northants
PAR-NEW-IN
1
6
0.02 %
0
0.00 %
5
83.33 %
2
33.33 %
1
16.67 %
0
0.00 %
Notts
PAR-NEW-IN
3
377
1.37 %
15
3.98 %
259
68.70 %
44
11.67 %
40
10.61 %
63
16.71 %
Oxon
PAR-NEW-IN
17
1,858
6.75 %
101
5.44 %
1570
84.50 %
198
10.66 %
187
10.06 %
0
0.00 %
Warks
PAR-NEW-IN
10
546
1.98 %
24
4.40 %
428
78.39 %
38
6.96 %
35
6.41 %
59
10.81 %
PAR-PERSIS
2
157
0.57 %
9
5.73 %
137
87.26 %
12
7.64 %
11
7.01 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
1
7
0.03 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
Cumbria
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
22
0.08 %
0
0.00 %
21
95.45 %
1
4.55 %
1
4.55 %
0
0.00 %
Lincs
PAR-NEW-IN
3
60
0.22 %
8
13.33 %
41
68.33 %
11
18.33 %
11
18.33 %
0
0.00 %
S Yorks
PAR-NEW-IN
1
162
0.59 %
4
2.47 %
150
92.59 %
10
6.17 %
8
4.94 %
0
0.00 %
England
162
20585
74.82 %
889
4.32 %
17501
85.02 %
1526
7.41 %
1335
6.49 %
860
4.18 %
Wales - High TB
Carms
PAR-HERD-S
5
455
1.65 %
38
8.35 %
401
88.13 %
24
5.27 %
15
3.30 %
1
0.22 %
PAR-PERSIS
17
2,952
10.73 %
133
4.51 %
2702
91.53 %
139
4.71 %
104
3.52 %
13
0.44 %
PAR-RAPID
11
63
0.23 %
25
39.68 %
34
53.97 %
8
12.70 %
4
6.35 %
0
0.00 %
SER-FLEXI
4
105
0.38 %
6
5.71 %
93
88.57 %
6
5.71 %
6
5.71 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
310
1.13 %
17
5.48 %
279
90.00 %
17
5.48 %
14
4.52 %
0
0.00 %
PAR-RAPID
4
5
0.02 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
3
392
1.42 %
18
4.59 %
347
88.52 %
31
7.91 %
26
6.63 %
1
0.26 %
PAR-RAPID
8
36
0.13 %
10
27.78 %
22
61.11 %
4
11.11 %
3
8.33 %
1
2.78 %
Pembs
PAR-HERD-S
3
49
0.18 %
3
6.12 %
45
91.84 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-OTHER
5
857
3.11 %
30
3.50 %
767
89.50 %
70
8.17 %
60
7.00 %
0
0.00 %
PAR-PERSIS
6
397
1.44 %
18
4.53 %
357
89.92 %
27
6.80 %
21
5.29 %
1
0.25 %
PAR-RAPID
13
33
0.12 %
7
21.21 %
25
75.76 %
2
6.06 %
1
3.03 %
0
0.00 %
SER-FLEXI
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
3
3
0.01 %
0
0.00 %
2
66.67 %
0
0.00 %
0
0.00 %
1
33.33 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
2
7
0.03 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-PERSIS
2
213
0.77 %
31
14.55 %
172
80.75 %
14
6.57 %
10
4.69 %
0
0.00 %
PAR-RAPID
4
34
0.12 %
4
11.76 %
11
32.35 %
2
5.88 %
2
5.88 %
17
50.00 %
SER-FLEXI
1
14
0.05 %
1
7.14 %
13
92.86 %
0
0.00 %
0
0.00 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
117
0.43 %
7
5.98 %
108
92.31 %
5
4.27 %
2
1.71 %
0
0.00 %
PAR-PERSIS
1
7
0.03 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
20
0.07 %
2
10.00 %
12
60.00 %
6
30.00 %
6
30.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
515
1.87 %
18
3.50 %
456
88.54 %
37
7.18 %
31
6.02 %
10
1.94 %
PAR-PERSIS
1
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
8
248
0.90 %
19
7.66 %
166
66.94 %
10
4.03 %
10
4.03 %
53
21.37 %
W Glamorgan
PAR-HERD-S
1
6
0.02 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
28
0.10 %
0
0.00 %
26
92.86 %
1
3.57 %
1
3.57 %
1
3.57 %
NE Wales
PAR-NEW-IN
4
46
0.17 %
0
0.00 %
31
67.39 %
1
2.17 %
0
0.00 %
15
32.61 %
Wales
127
6928
25.18 %
394
5.69 %
6098
88.02 %
411
5.93 %
322
4.65 %
114
1.65 %
Grand Total
Sum:
289
27513
1283
23599
1937
1657
974
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol January 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
156
20499
99.6%
883
4.3%
17453
85.1%
1525
7.4%
1334
6.5%
829
4.0%
Serial/Extended
6
86
0.4%
6
7.0%
48
55.8%
1
1.2%
1
1.2%
31
36.0%
Total
162
20585
100.0%
889
4.3%
17501
85.0%
1526
7.4%
1335
6.5%
860
4.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
112
6557
94.6%
367
5.6%
5823
88.8%
395
6.0%
306
4.7%
61
0.9%
Serial/Extended
15
371
5.4%
27
7.3%
275
74.1%
16
4.3%
16
4.3%
53
14.3%
Total
127
6928
100.0%
394
5.7%
6098
88.0%
411
5.9%
322
4.6%
114
1.6%
GB
Parallel
268
27056
98.3%
1250
4.6%
23276
86.0%
1920
7.1%
xx-xxxx-xxxx
6.1%
890
3.3%
Serial/Extended
21
457
1.7%
33
7.2%
323
70.7%
17
3.7%
17
3.7%
84
18.4%
Total
289
27513
100.0%
1283
4.7%
23599
85.8%
1937
7.0%
1657
6.0%
974
3.5%
162.00
127.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
156
20499
99.6%
883
4.3%
17453
85.1%
1525
7.4%
1334
6.5%
829
4.0%
Serial/Extended
6
86
0.4%
6
7.0%
48
55.8%
1
1.2%
1
1.2%
31
36.0%
Total
162
20585
100.0%
889
4.3%
17501
85.0%
1526
7.4%
1335
6.5%
860
4.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
112
6557
94.6%
367
5.6%
5823
88.8%
395
6.0%
306
4.7%
61
0.9%
Serial/Extended
15
371
5.4%
27
7.3%
275
74.1%
16
4.3%
16
4.3%
53
14.3%
Total
127
6928
100.0%
394
5.7%
6098
88.0%
411
5.9%
322
4.6%
114
1.6%
GB
Parallel
268
27056
98.3%
1250
4.6%
23276
86.0%
1920
7.1%
xx-xxxx-xxxx
6.1%
890
3.3%
Serial/Extended
21
457
1.7%
33
7.2%
323
70.7%
17
3.7%
17
3.7%
84
18.4%
Total
289
27513
100.0%
1283
4.7%
23599
85.8%
1937
7.0%
1657
6.0%
974
3.5%
162.00
127.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
450
1.6%
33
7.3%
316
70.2%
17
3.8 %
17
3.8 %
84
18.7 %
Parallel - Herd or Group Slaughter
16
634
2.3%
50
7.9%
563
88.8%
31
4.9 %
19
3.0 %
2
0.3 %
Parallel New Infection
119
13956
50.7%
534
3.8%
11923
85.4%
1004
7.2 %
888
6.4 %
611
4.4 %
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
11
1605
5.8%
91
5.7%
1359
84.7%
132
8.2 %
114
7.1 %
41
2.6 %
Parallel Persistent Infection
46
5313
19.3%
271
5.1%
4668
87.9%
322
6.1 %
262
4.9 %
112
2.1 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
Parallel - Rapid Testing Two-times IRs
41
173
0.6%
50
28.9%
95
54.9%
17
9.8 %
10
5.8 %
18
10.4 %
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
289
27513
100.0%
1283
4.7%
23599
85.8%
1937
7.0 %
1657
6.0 %
974
3.5 %
Table 4a. Summaryof IFN Gamma results by submission reason (January 2019)
England - HRA
Extended Flexible IFN-GAMMA test
5
79
0.3%
6
7.6%
41
51.9%
1
1.3 %
1
1.3 %
31
39.2 %
Parallel New Infection
1
344
1.3%
67
19.5%
242
70.3%
42
12.2 %
35
10.2 %
0
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
5
728
2.6%
59
8.1%
580
79.7%
56
7.7 %
48
6.6 %
41
5.6 %
Parallel Persistent Infection
8
774
2.8%
40
5.2%
564
72.9%
78
10.1 %
73
9.4 %
97
12.5 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
England - HRA
54
7300
26.5%
426
5.8%
6095
83.5%
591
8.1 %
504
6.9 %
275
3.8 %
England - Edge
Parallel New Infection
96
12762
46.4%
437
3.4%
10944
85.8%
896
7.0 %
796
6.2 %
585
4.6 %
Parallel Persistent Infection
4
262
1.0%
14
5.3%
236
90.1%
14
5.3 %
12
4.6 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - Edge
101
13031
47.4%
451
3.5%
11187
85.8%
910
7.0 %
808
6.2 %
585
4.5 %
England - LRA
Parallel New Infection
7
254
0.9%
12
4.7%
219
86.2%
25
9.8 %
23
9.1 %
0
England - LRA
7
254
0.9%
12
4.7%
219
86.2%
25
9.8 %
23
9.1 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
7
123
0.4%
8
6.5%
109
88.6%
6
4.9 %
6
4.9 %
0
Parallel - Herd or Group Slaughter
14
627
2.3%
48
7.7%
559
89.2%
30
4.8 %
18
2.9 %
2
0.3 %
Parallel New Infection
2
7
0.0%
0
0.0 %
5
71.4%
2
28.6 %
2
28.6 %
0
Parallel - Other
5
857
3.1%
30
3.5%
767
89.5%
70
8.2 %
60
7.0 %
0
Parallel Persistent Infection
33
4271
15.5%
217
5.1%
3862
90.4%
230
5.4 %
177
4.1 %
15
0.4 %
Parallel - Rapid Testing Two-times IRs
40
171
0.6%
50
29.2%
93
54.4%
17
9.9 %
10
5.8 %
18
10.5 %
Wales - High TB
101
6056
22.0%
353
5.8%
5395
89.1%
355
5.9 %
273
4.5 %
35
0.6 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
8
248
0.9%
19
7.7%
166
66.9%
10
4.0 %
10
4.0 %
53
21.4 %
Parallel - Herd or Group Slaughter
2
7
0.0%
2
28.6%
4
57.1%
1
14.3 %
1
14.3 %
0
Parallel New Infection
7
515
1.9%
18
3.5%
456
88.5%
37
7.2 %
31
6.0 %
10
1.9 %
Parallel - Other
1
20
0.1%
2
10.0%
12
60.0%
6
30.0 %
6
30.0 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Intermediate TB
20
798
2.9%
41
5.1%
646
81.0%
54
6.8 %
48
6.0 %
63
7.9 %
Wales - Low TB
Parallel New Infection
6
74
0.3%
0
0.0 %
57
77.0%
2
2.7 %
1
1.4 %
16
21.6 %
Wales - Low TB
6
74
0.3%
0
0.0 %
57
77.0%
2
2.7 %
1
1.4 %
16
21.6 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
450
1.6%
33
7.3%
316
70.2%
17
3.8 %
17
3.8 %
84
18.7 %
Parallel - Herd or Group Slaughter
16
634
2.3%
50
7.9%
563
88.8%
31
4.9 %
19
3.0 %
2
0.3 %
Parallel New Infection
119
13956
50.7%
534
3.8%
11923
85.4%
1004
7.2 %
888
6.4 %
611
4.4 %
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
11
1605
5.8%
91
5.7%
1359
84.7%
132
8.2 %
114
7.1 %
41
2.6 %
Parallel Persistent Infection
46
5313
19.3%
271
5.1%
4668
87.9%
322
6.1 %
262
4.9 %
112
2.1 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
Parallel - Rapid Testing Two-times IRs
41
173
0.6%
50
28.9%
95
54.9%
17
9.8 %
10
5.8 %
18
10.4 %
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
289
27513
100.0%
1283
4.7%
23599
85.8%
1937
7.0 %
1657
6.0 %
974
3.5 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
5
79
0.3%
6
7.6%
41
51.9%
1
1.3 %
1
1.3 %
31
39.2 %
Parallel New Infection
1
344
1.3%
67
19.5%
242
70.3%
42
12.2 %
35
10.2 %
0
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
5
728
2.6%
59
8.1%
580
79.7%
56
7.7 %
48
6.6 %
41
5.6 %
Parallel Persistent Infection
8
774
2.8%
40
5.2%
564
72.9%
78
10.1 %
73
9.4 %
97
12.5 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
England - Edge
Parallel New Infection
96
12762
46.4%
437
3.4%
10944
85.8%
896
7.0 %
796
6.2 %
585
4.6 %
Parallel Persistent Infection
4
262
1.0%
14
5.3%
236
90.1%
14
5.3 %
12
4.6 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - LRA
Parallel New Infection
7
254
0.9%
12
4.7%
219
86.2%
25
9.8 %
23
9.1 %
0
England
162
20585
74.8%
889
4.3%
17501
85.0%
1526
7.4 %
1335
6.5 %
860
4.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
7
123
0.4%
8
6.5%
109
88.6%
6
4.9 %
6
4.9 %
0
Parallel - Herd or Group Slaughter
14
627
2.3%
48
7.7%
559
89.2%
30
4.8 %
18
2.9 %
2
0.3 %
Parallel New Infection
2
7
0.0%
0
0.0 %
5
71.4%
2
28.6 %
2
28.6 %
0
Parallel - Other
5
857
3.1%
30
3.5%
767
89.5%
70
8.2 %
60
7.0 %
0
Parallel Persistent Infection
33
4271
15.5%
217
5.1%
3862
90.4%
230
5.4 %
177
4.1 %
15
0.4 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Parallel - Rapid Testing Two-times IRs
40
171
0.6%
50
29.2%
93
54.4%
17
9.9 %
10
5.8 %
18
10.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
8
248
0.9%
19
7.7%
166
66.9%
10
4.0 %
10
4.0 %
53
21.4 %
Parallel - Herd or Group Slaughter
2
7
0.0%
2
28.6%
4
57.1%
1
14.3 %
1
14.3 %
0
Parallel New Infection
7
515
1.9%
18
3.5%
456
88.5%
37
7.2 %
31
6.0 %
10
1.9 %
Parallel - Other
1
20
0.1%
2
10.0%
12
60.0%
6
30.0 %
6
30.0 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Low TB
Parallel New Infection
6
74
0.3%
0
0.0 %
57
77.0%
2
2.7 %
1
1.4 %
16
21.6 %
Wales
127
6928
25.2%
394
5.7%
6098
88.0%
411
5.9 %
322
4.6 %
114
1.6 %

Table 5a. Summary of PM and culture results for IFNg positive animals.
Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 February
206
20791
130
1229
3
22
0
0
0
0
72
7
1
8
0
1073
1
35
0
5
March
189
17292
131
1199
1
3
0
0
0
0
121
6
0
4
0
984
0
72
1
2
April
195
20517
118
953
4
16
0
0
0
0
116
1
0
0
0
748
0
66
1
1
May
250
21066
131
947
2
2
0
0
0
0
121
1
0
4
0
780
0
35
1
1
June
187
18891
110
701
2
2
0
0
0
0
78
2
0
12
0
579
0
25
0
0
July
207
24111
119
806
1
12
0
0
0
0
67
4
0
2
0
675
3
38
0
2
August
228
19599
120
682
4
3
0
0
0
0
64
2
0
8
1
569
0
31
0
0
September
216
21441
137
946
6
6
0
0
0
0
33
1
0
2
0
840
0
51
1
4
October
274
23976
152
1417
12
8
0
0
0
0
99
1
0
3
1
1231
5
40
0
1
November
282
24570
192
1580
2
5
0
0
0
0
42
0
4
7
4
1329
84
87
0
7
December
228
21554
147
1241
6
21
0
0
0
0
91
0
8
2
0
1025
87
0
0
0
2019 January
289
27513
179
1283
474
412
0
0
0
0
26
0
3
0
0
237
14
0
0
0
Totals (last
12 months)
2751
261321
1666
12984
517
512
0
0
0
0
930
25
16
52
6
10070
194
480
4
23

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)
Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1530
200003
1049
9852
271
0
0
0
0
775
15
7
34
4
8193
25
51
1
3
Scotland
26
1828
17
146
30
0
0
0
0
22
4
0
0
0
49
0
36
0
3
Wales
1195
59490
600
2986
211
0
0
0
0
133
6
9
18
2
1828
169
393
3
17
Totals
2751
261321
1666
12984
512
0
0
0
0
930
25
16
52
6
10070
194
480
4
23

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
0
5000
10000
15000
20000
25000
30000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
Warks
S Wales
Shropshire
Pembs
Notts
NE Wales
Leics & Rut
Heref
Gwynedd
Gloucs
Dorset
Derbyshire
Corn & Scilly
Ceredigion
Cambs
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during January 2019 (% submission per
County)
County
%Resampled %Retested
Avon
8.50 %
10.00 %
Berks
5.99 %
6.72 %
Bucks
1.96 %
4.58 %
Cambs
28.57 %
28.57 %
Carms
3.76 %
5.09 %
Ceredigion
4.73 %
5.99 %
Cheshire
5.31 %
6.27 %
Corn & Scilly
7.19 %
8.91 %
Cumbria
33.33 %
33.33 %
Derbyshire
4.79 %
5.51 %
Devon
7.21 %
8.38 %
Dorset
6.09 %
6.26 %
E Sussex
3.59 %
3.72 %
Gloucs
7.10 %
8.55 %
Gwent
6.78 %
8.18 %
Gwynedd
3.57 %
3.57 %
Hampshire
4.84 %
4.84 %
Heref
4.96 %
5.25 %
Lancashire
4.55 %
4.55 %
Leics & Rut
10.53 %
11.37 %
Lincs
18.33 %
18.33 %
NE Wales
5.02 %
5.88 %
Northants
16.67 %
33.33 %
Notts
10.61 %
11.67 %
Oxon
10.06 %
10.66 %
Pembs
6.20 %
7.47 %
Powys
5.17 %
6.64 %
Shropshire
9.89 %
11.22 %
Somerset
3.65 %
5.36 %
S Wales
S Yorks
4.94 %
6.17 %
Warks
6.54 %
7.11 %
W Glamorgan
3.08 %
5.38 %
Wiltshire
7.89 %
13.16 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
Table 6a (i): Number of animals and submissions tested in January 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
14147
657
4.6%
14220
657
4.6%
Wales
4813
277
5.8%
5045
277
5.5%
Scotland
410
35
8.5%
413
35
8.5%
GB
19370
969
5.0%
19678
969
4.9%
Table 6a (ii): Number of animals and submissions tested in January 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
14147
657
4.6%
14220
657
4.6%
Wales
4813
277
5.8%
5045
277
5.5%
Scotland
410
35
8.5%
413
35
8.5%
GB
19370
969
5.0%
19678
969
4.9%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Gloucestershire
PRV-SP
1
4
0
Somerset excl North
PRV-SE
1
4
1
25.00 %
Wiltshire
PRV-SP
1
3
0
Total
3
11
1
9.09 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples January 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Gloucestershire
PRV-SP
1
4
0
Somerset excl North
PRV-SE
1
4
1
25.00 %
Wiltshire
PRV-SP
1
3
0
Total
3
11
1
9.09 %
Isle of Man
SER-IOM
3
19
0
Isle of Man
SER-IOM
3
19
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
North East Wales
2
97
5
5.2 %
TC0077
Sum:
97
5
5.2 %
Table 8a Number of Cattle Antibody Tests January 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
North East Wales
2
97
5
5.2 %
TC0077
Sum:
97
5
5.2 %
Table 8b Number of Cattle Antibody Tests 2019