Monthly report: Suveillence Project SB4008 IFNg tests for Bovine tuberculosis - October 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 158
Report Period 1st - 31st October 2019
Issued 10 November 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
October
2019
%
Total 2019
%
Submissions
298
2965
Samples
Total (%)
26690
100.00 %
276588
100.00 %
England
20315
76.11 %
221109
79.94 %
Wales
5904
22.12 %
52791
19.09 %
Scotland
471
1.76 %
2688
0.97 %
Parallel Tests
PAR-CUL-N
5925
22.20 %
60714
21.95 %
PAR-CUL-P
1760
6.59 %
17715
6.40 %
PAR-HERD-S
2536
9.50 %
7342
2.65 %
PAR-NEW-IN
12721
47.66 %
126794
45.84 %
PAR-OTHER
61
0.23 %
4174
1.51 %
PAR-PERSIS
3206
12.01 %
52750
19.07 %
PAR-RAPID
301
1.13 %
1250
0.45 %
Total(% of all samples)
26510
99.33 %
270739
97.89 %
Serial/Extended Tests
SER-FLEXI
177
0.66 %
5820
2.10 %
SER-FRAUD
0
0.00 %
18
0.01 %
SER-NSR
3
0.01 %
11
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
180
0.67 %
5849
2.11 %
Total
26690
100.00 %
276588
100.00 %
Retests
Total (% of all samples)
xx-xxxx-xxxx
6.15 %
19554
7.07 %
England
1296
4.86 %
16244
5.87 %
Wales
321
1.20 %
3183
1.15 %
Scotland
24
0.09 %
127
0.05 %
Resamples
Total (% of all samples)
1293
4.84 %
15224
5.50 %
England
1046
3.92 %
12684
4.59 %
Wales
228
0.85 %
2436
0.88 %
Scotland
19
0.07 %
104
0.04 %
Rejects
Total (% of all samples)
750
2.81 %
2738
0.99 %
England
715
2.68 %
2521
0.91 %
Wales
25
0.09 %
207
0.07 %
Scotland
10
0.04 %
10
0.00 %
Table 1. Monthly Totals for October 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CUL-N
5925
22.2 %
PAR-CUL-P
1760
6.6 %
PAR-HERD-S
2536
9.5 %
PAR-NEW-IN
12721
47.7 %
PAR-OTHER
61
0.2 %
PAR-PERSIS
3206
12.0 %
PAR-RAPID
301
1.1 %
SER-FLEXI
177
0.7 %
SER-NSR
3
0.0 %
Total
26690
100.00 %
Figure 1. Number of samples submitted in each category during October 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Kirkcudbright
Dumfries & G
W Glamorgan
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Suffolk
Somerset excl N
Shropshire
Oxon
Northumb
Northants
Leics & Rut
Heref
Hampshire
Gloucs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Scotland
Wales
England
SER-NSR
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during October 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Lincs
Leics & Rut
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gr London
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
3
96
0.36 %
2
2.08 %
93
96.88 %
2
2.08 %
1
1.04 %
0
0.00 %
PAR-PERSIS
2
534
2.00 %
24
4.49 %
491
91.95 %
20
3.75 %
19
3.56 %
0
0.00 %
SER-FLEXI
2
28
0.10 %
0
0.00 %
26
92.86 %
2
7.14 %
2
7.14 %
0
0.00 %
Devon
PAR-CUL-N
23
2,827
10.59 %
82
2.90 %
2409
85.21 %
252
8.91 %
180
6.37 %
156
5.52 %
PAR-CUL-P
1
174
0.65 %
1
0.57 %
162
93.10 %
15
8.62 %
11
6.32 %
0
0.00 %
PAR-PERSIS
3
508
1.90 %
19
3.74 %
454
89.37 %
41
8.07 %
35
6.89 %
0
0.00 %
Dorset
PAR-CUL-N
6
492
1.84 %
9
1.83 %
449
91.26 %
45
9.15 %
34
6.91 %
0
0.00 %
PAR-CUL-P
4
311
1.17 %
16
5.14 %
281
90.35 %
16
5.14 %
14
4.50 %
0
0.00 %
PAR-NEW-IN
2
14
0.05 %
0
0.00 %
12
85.71 %
2
14.29 %
2
14.29 %
0
0.00 %
Gloucs
PAR-CUL-N
3
863
3.23 %
27
3.13 %
809
93.74 %
32
3.71 %
26
3.01 %
1
0.12 %
Heref
PAR-CUL-P
2
236
0.88 %
12
5.08 %
214
90.68 %
14
5.93 %
10
4.24 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
6
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
6
100.00 %
Shropshire
PAR-OTHER
1
5
0.02 %
0
0.00 %
2
40.00 %
3
60.00 %
3
60.00 %
0
0.00 %
Somerset excl N
PAR-CUL-N
8
360
1.35 %
15
4.17 %
285
79.17 %
25
6.94 %
25
6.94 %
35
9.72 %
Wiltshire
PAR-CUL-N
10
1,287
4.82 %
14
1.09 %
1194
92.77 %
91
7.07 %
78
6.06 %
1
0.08 %
PAR-CUL-P
5
687
2.57 %
43
6.26 %
612
89.08 %
38
5.53 %
32
4.66 %
0
0.00 %
PAR-PERSIS
1
7
0.03 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
7
100.00 %
England - Edge
Bucks
PAR-NEW-IN
3
391
1.46 %
9
2.30 %
372
95.14 %
12
3.07 %
9
2.30 %
1
0.26 %
Cheshire
PAR-NEW-IN
24
5,593
20.96 %
95
1.70 %
4895
87.52 %
211
3.77 %
179
3.20 %
424
7.58 %
Derbyshire
PAR-NEW-IN
22
2,255
8.45 %
41
1.82 %
2032
90.11 %
135
5.99 %
106
4.70 %
76
3.37 %
Hampshire
PAR-NEW-IN
3
70
0.26 %
1
1.43 %
64
91.43 %
5
7.14 %
5
7.14 %
0
0.00 %
Leics & Rut
PAR-PERSIS
1
336
1.26 %
20
5.95 %
305
90.77 %
11
3.27 %
8
2.38 %
3
0.89 %
SER-NSR
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
1
93
0.35 %
1
1.08 %
90
96.77 %
3
3.23 %
2
2.15 %
0
0.00 %
Oxon
PAR-NEW-IN
14
1,428
5.35 %
35
2.45 %
1265
88.59 %
149
10.43 %
123
8.61 %
5
0.35 %
Table 2a. Summary by County for October 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
339
1.27 %
5
1.47 %
312
92.04 %
28
8.26 %
22
6.49 %
0
0.00 %
Warks
PAR-NEW-IN
11
442
1.66 %
5
1.13 %
419
94.80 %
23
5.20 %
18
4.07 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
1
11
0.04 %
0
0.00 %
8
72.73 %
3
27.27 %
3
27.27 %
0
0.00 %
Cumbria
PAR-NEW-IN
4
394
1.48 %
0
0.00 %
345
87.56 %
56
14.21 %
49
12.44 %
0
0.00 %
PAR-PERSIS
1
135
0.51 %
4
2.96 %
121
89.63 %
19
14.07 %
10
7.41 %
0
0.00 %
Northumb
PAR-NEW-IN
1
91
0.34 %
0
0.00 %
88
96.70 %
3
3.30 %
3
3.30 %
0
0.00 %
Suffolk
PAR-NEW-IN
2
298
1.12 %
3
1.01 %
258
86.58 %
40
13.42 %
37
12.42 %
0
0.00 %
England
169
20315
76.11 %
483
2.38 %
18071
88.95 %
1296
6.38 %
1046
5.15 %
715
3.52 %
Wales - High TB
Carms
PAR-HERD-S
3
578
2.17 %
31
5.36 %
517
89.45 %
42
7.27 %
30
5.19 %
0
0.00 %
PAR-OTHER
2
17
0.06 %
12
70.59 %
4
23.53 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-PERSIS
8
171
0.64 %
28
16.37 %
139
81.29 %
6
3.51 %
4
2.34 %
0
0.00 %
PAR-RAPID
6
28
0.10 %
5
17.86 %
23
82.14 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
71
0.27 %
5
7.04 %
65
91.55 %
2
2.82 %
1
1.41 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
29
0.11 %
4
13.79 %
21
72.41 %
5
17.24 %
4
13.79 %
0
0.00 %
PAR-RAPID
9
20
0.07 %
4
20.00 %
16
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-OTHER
1
9
0.03 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
143
0.54 %
8
5.59 %
132
92.31 %
5
3.50 %
3
2.10 %
0
0.00 %
PAR-RAPID
2
9
0.03 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
Pembs
PAR-HERD-S
10
1,944
7.28 %
36
1.85 %
1867
96.04 %
64
3.29 %
38
1.95 %
3
0.15 %
PAR-OTHER
2
5
0.02 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
47
0.18 %
5
10.64 %
41
87.23 %
3
6.38 %
1
2.13 %
0
0.00 %
PAR-RAPID
15
60
0.22 %
8
13.33 %
38
63.33 %
3
5.00 %
2
3.33 %
12
20.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
1
14
0.05 %
1
7.14 %
13
92.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
5
398
1.49 %
25
6.28 %
363
91.21 %
12
3.02 %
10
2.51 %
0
0.00 %
PAR-PERSIS
6
591
2.21 %
19
3.21 %
547
92.55 %
25
4.23 %
18
3.05 %
7
1.18 %
PAR-RAPID
5
32
0.12 %
4
12.50 %
22
68.75 %
7
21.88 %
6
18.75 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
3
46
0.17 %
4
8.70 %
40
86.96 %
4
8.70 %
2
4.35 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
7
0.03 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
314
1.18 %
4
1.27 %
286
91.08 %
30
9.55 %
21
6.69 %
3
0.96 %
PAR-OTHER
2
7
0.03 %
0
0.00 %
3
42.86 %
4
57.14 %
4
57.14 %
0
0.00 %
PAR-PERSIS
2
130
0.49 %
6
4.62 %
117
90.00 %
12
9.23 %
7
5.38 %
0
0.00 %
PAR-RAPID
2
135
0.51 %
1
0.74 %
129
95.56 %
5
3.70 %
5
3.70 %
0
0.00 %
SER-FLEXI
3
29
0.11 %
0
0.00 %
28
96.55 %
1
3.45 %
1
3.45 %
0
0.00 %
Powys
PAR-NEW-IN
1
250
0.94 %
2
0.80 %
237
94.80 %
12
4.80 %
11
4.40 %
0
0.00 %
S Glamorgan
PAR-RAPID
2
8
0.03 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
165
0.62 %
10
6.06 %
144
87.27 %
13
7.88 %
11
6.67 %
0
0.00 %
W Glamorgan
PAR-PERSIS
1
230
0.86 %
15
6.52 %
199
86.52 %
22
9.57 %
16
6.96 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
5
395
1.48 %
5
1.27 %
364
92.15 %
40
10.13 %
30
7.59 %
0
0.00 %
PAR-OTHER
1
17
0.06 %
0
0.00 %
17
100.00 %
1
5.88 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
124
5904
22.12 %
253
4.29 %
5402
91.50 %
321
5.44 %
228
3.86 %
25
0.42 %
Scotland - Scotland
Dumfries & G
PAR-NEW-IN
3
114
0.43 %
0
0.00 %
93
81.58 %
13
11.40 %
11
9.65 %
10
8.77 %
Kirkcudbright
PAR-CUL-P
1
352
1.32 %
7
1.99 %
340
96.59 %
8
2.27 %
5
1.42 %
0
0.00 %
PAR-NEW-IN
1
5
0.02 %
0
0.00 %
2
40.00 %
3
60.00 %
3
60.00 %
0
0.00 %
Scotland
5
471
1.76 %
7
1.49 %
435
92.36 %
24
5.10 %
19
4.03 %
10
2.12 %
Grand Total
Sum:
298
26690
743
23908
xx-xxxx-xxxx
1293
750
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
3
431
0.16 %
9
2.09 %
407
94.43 %
23
5.34 %
15
3.48 %
0
0.00 %
PAR-OTHER
4
83
0.03 %
1
1.20 %
70
84.34 %
12
14.46 %
12
14.46 %
0
0.00 %
PAR-PERSIS
9
1,490
0.54 %
45
3.02 %
1387
93.09 %
72
4.83 %
56
3.76 %
2
0.13 %
SER-FRAUD
2
18
0.01 %
0
0.00 %
17
94.44 %
1
5.56 %
1
5.56 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
44
4,302
1.56 %
141
3.28 %
3839
89.24 %
335
7.79 %
271
6.30 %
51
1.19 %
PAR-CUL-P
4
1,165
0.42 %
19
1.63 %
1035
88.84 %
132
11.33 %
109
9.36 %
2
0.17 %
PAR-NEW-IN
7
793
0.29 %
14
1.77 %
699
88.15 %
103
12.99 %
80
10.09 %
0
0.00 %
PAR-PERSIS
33
7,929
2.87 %
327
4.12 %
6972
87.93 %
440
5.55 %
335
4.22 %
295
3.72 %
SER-FLEXI
4
43
0.02 %
0
0.00 %
41
95.35 %
2
4.65 %
2
4.65 %
0
0.00 %
Devon
PAR-CUL-N
199
26,591
9.61 %
1296
4.87 %
23647
88.93 %
1845
6.94 %
1385
5.21 %
263
0.99 %
PAR-CUL-P
28
6,779
2.45 %
313
4.62 %
6094
89.90 %
461
6.80 %
368
5.43 %
4
0.06 %
PAR-NEW-IN
4
674
0.24 %
106
15.73 %
501
74.33 %
86
12.76 %
67
9.94 %
0
0.00 %
PAR-OTHER
7
532
0.19 %
6
1.13 %
352
66.17 %
202
37.97 %
174
32.71 %
0
0.00 %
PAR-PERSIS
22
2,854
1.03 %
181
6.34 %
2492
87.32 %
231
8.09 %
180
6.31 %
1
0.04 %
SER-FLEXI
8
849
0.31 %
50
5.89 %
732
86.22 %
47
5.54 %
22
2.59 %
45
5.30 %
Dorset
PAR-CUL-N
94
12,038
4.35 %
632
5.25 %
10698
88.87 %
849
7.05 %
652
5.42 %
56
0.47 %
PAR-CUL-P
17
3,207
1.16 %
250
7.80 %
2843
88.65 %
156
4.86 %
114
3.55 %
0
0.00 %
PAR-NEW-IN
5
55
0.02 %
1
1.82 %
43
78.18 %
11
20.00 %
11
20.00 %
0
0.00 %
SER-FLEXI
4
153
0.06 %
6
3.92 %
137
89.54 %
11
7.19 %
10
6.54 %
0
0.00 %
Gloucs
PAR-CUL-N
52
4,175
1.51 %
156
3.74 %
3588
85.94 %
385
9.22 %
322
7.71 %
109
2.61 %
PAR-CUL-P
10
1,278
0.46 %
52
4.07 %
1115
87.25 %
140
10.95 %
111
8.69 %
0
0.00 %
PAR-HERD-S
4
33
0.01 %
0
0.00 %
31
93.94 %
3
9.09 %
2
6.06 %
0
0.00 %
PAR-NEW-IN
1
112
0.04 %
3
2.68 %
103
91.96 %
7
6.25 %
6
5.36 %
0
0.00 %
SER-FLEXI
2
30
0.01 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
15
1,724
0.62 %
69
4.00 %
1541
89.39 %
129
7.48 %
103
5.97 %
11
0.64 %
PAR-CUL-P
4
418
0.15 %
16
3.83 %
373
89.23 %
36
8.61 %
29
6.94 %
0
0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
1
11
0.00 %
0
0.00 %
10
90.91 %
4
36.36 %
1
9.09 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
30
3,412
1.23 %
138
4.04 %
3062
89.74 %
252
7.39 %
200
5.86 %
12
0.35 %
Shropshire
PAR-CUL-P
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
7
635
0.23 %
43
6.77 %
545
85.83 %
60
9.45 %
46
7.24 %
1
0.16 %
PAR-PERSIS
10
935
0.34 %
26
2.78 %
809
86.52 %
111
11.87 %
99
10.59 %
1
0.11 %
SER-FLEXI
5
557
0.20 %
21
3.77 %
509
91.38 %
34
6.10 %
27
4.85 %
0
0.00 %
Somerset excl N
PAR-CUL-N
46
4,864
1.76 %
220
4.52 %
4375
89.95 %
352
7.24 %
232
4.77 %
37
0.76 %
PAR-OTHER
2
238
0.09 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
13
1,907
0.69 %
110
5.77 %
1510
79.18 %
105
5.51 %
69
3.62 %
218
11.43 %
Staffs
PAR-OTHER
2
140
0.05 %
3
2.14 %
125
89.29 %
15
10.71 %
12
8.57 %
0
0.00 %
PAR-PERSIS
7
1,492
0.54 %
202
13.54 %
1201
80.50 %
130
8.71 %
90
6.03 %
0
0.00 %
Wiltshire
PAR-CUL-N
65
6,722
2.43 %
233
3.47 %
6020
89.56 %
514
7.65 %
437
6.50 %
32
0.48 %
PAR-CUL-P
24
4,076
1.47 %
240
5.89 %
3654
89.65 %
248
6.08 %
181
4.44 %
1
0.02 %
PAR-NEW-IN
7
456
0.16 %
15
3.29 %
398
87.28 %
53
11.62 %
43
9.43 %
0
0.00 %
PAR-OTHER
2
37
0.01 %
1
2.70 %
34
91.89 %
4
10.81 %
2
5.41 %
0
0.00 %
PAR-PERSIS
10
2,038
0.74 %
82
4.02 %
1875
92.00 %
109
5.35 %
69
3.39 %
12
0.59 %
SER-FLEXI
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Worcs
PAR-PERSIS
1
41
0.01 %
0
0.00 %
41
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
39
4,468
1.62 %
155
3.47 %
4016
89.88 %
362
8.10 %
297
6.65 %
0
0.00 %
PAR-PERSIS
3
204
0.07 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
28
2,794
1.01 %
94
3.36 %
2472
88.48 %
261
9.34 %
212
7.59 %
16
0.57 %
Cheshire
PAR-CUL-N
2
298
0.11 %
21
7.05 %
258
86.58 %
21
7.05 %
19
6.38 %
0
0.00 %
PAR-NEW-IN
209
43,223
15.63 %
971
2.25 %
39797
92.07 %
2549
5.90 %
1880
4.35 %
575
1.33 %
PAR-PERSIS
5
540
0.20 %
3
0.56 %
512
94.81 %
29
5.37 %
25
4.63 %
0
0.00 %
Derbyshire
PAR-NEW-IN
157
17,289
6.25 %
549
3.18 %
15296
88.47 %
1393
8.06 %
1155
6.68 %
289
1.67 %
E Sussex
PAR-NEW-IN
21
2,721
0.98 %
125
4.59 %
2450
90.04 %
157
5.77 %
149
5.48 %
13
0.48 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-NEW-IN
36
3,024
1.09 %
71
2.35 %
2739
90.58 %
270
8.93 %
216
7.14 %
0
0.00 %
PAR-PERSIS
1
37
0.01 %
2
5.41 %
28
75.68 %
9
24.32 %
7
18.92 %
0
0.00 %
SER-NSR
2
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
54
6,278
2.27 %
261
4.16 %
5572
88.75 %
434
6.91 %
350
5.58 %
95
1.51 %
PAR-PERSIS
1
336
0.12 %
20
5.95 %
305
90.77 %
11
3.27 %
8
2.38 %
3
0.89 %
SER-NSR
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
28
1,981
0.72 %
118
5.96 %
1778
89.75 %
132
6.66 %
85
4.29 %
0
0.00 %
Notts
PAR-NEW-IN
7
774
0.28 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
134
12,588
4.55 %
498
3.96 %
11158
88.64 %
1110
8.82 %
877
6.97 %
55
0.44 %
PAR-PERSIS
2
339
0.12 %
5
1.47 %
312
92.04 %
28
8.26 %
22
6.49 %
0
0.00 %
Warks
PAR-NEW-IN
98
7,972
2.88 %
335
4.20 %
7087
88.90 %
585
7.34 %
464
5.82 %
86
1.08 %
PAR-PERSIS
6
570
0.21 %
19
3.33 %
526
92.28 %
32
5.61 %
25
4.39 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
7
409
0.15 %
22
5.38 %
355
86.80 %
30
7.33 %
24
5.87 %
8
1.96 %
Cumbria
PAR-NEW-IN
21
3,783
1.37 %
110
2.91 %
3430
90.67 %
325
8.59 %
243
6.42 %
0
0.00 %
PAR-PERSIS
1
135
0.05 %
4
2.96 %
121
89.63 %
19
14.07 %
10
7.41 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.03 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr London
PAR-NEW-IN
2
45
0.02 %
0
0.00 %
42
93.33 %
6
13.33 %
3
6.67 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
79
0.03 %
6
7.59 %
52
65.82 %
25
31.65 %
21
26.58 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.37 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
4
641
0.23 %
16
2.50 %
562
87.68 %
69
10.76 %
62
9.67 %
1
0.16 %
Kent
PAR-NEW-IN
1
10
0.00 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
106
0.04 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
11
1,074
0.39 %
24
2.23 %
937
87.24 %
122
11.36 %
113
10.52 %
0
0.00 %
Northumb
PAR-NEW-IN
3
296
0.11 %
0
0.00 %
241
81.42 %
63
21.28 %
55
18.58 %
0
0.00 %
PAR-PERSIS
3
183
0.07 %
1
0.55 %
32
17.49 %
58
31.69 %
57
31.15 %
93
50.82 %
N Yorks
PAR-NEW-IN
7
875
0.32 %
22
2.51 %
766
87.54 %
71
8.11 %
59
6.74 %
28
3.20 %
PAR-PERSIS
3
575
0.21 %
2
0.35 %
551
95.83 %
31
5.39 %
22
3.83 %
0
0.00 %
SER-FLEXI
2
50
0.02 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Suffolk
PAR-NEW-IN
2
298
0.11 %
3
1.01 %
258
86.58 %
40
13.42 %
37
12.42 %
0
0.00 %
S Yorks
PAR-NEW-IN
5
404
0.15 %
7
1.73 %
381
94.31 %
21
5.20 %
15
3.71 %
1
0.25 %
W Sussex
PAR-NEW-IN
2
239
0.09 %
2
0.84 %
163
68.20 %
81
33.89 %
74
30.96 %
0
0.00 %
W Yorks
PAR-HERD-S
2
24
0.01 %
8
33.33 %
14
58.33 %
2
8.33 %
2
8.33 %
0
0.00 %
England
1747
221109
79.94 %
8632
3.90 %
197291
89.23 %
16244
7.35 %
12684
5.74 %
2521
1.14 %
Wales - High TB
Carms
PAR-HERD-S
55
3,150
1.14 %
207
6.57 %
2774
88.06 %
216
6.86 %
167
5.30 %
2
0.06 %
PAR-NEW-IN
3
121
0.04 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
16
464
0.17 %
70
15.09 %
384
82.76 %
20
4.31 %
10
2.16 %
0
0.00 %
PAR-PERSIS
72
6,439
2.33 %
350
5.44 %
5836
90.64 %
327
5.08 %
242
3.76 %
13
0.20 %
PAR-RAPID
68
286
0.10 %
72
25.17 %
200
69.93 %
23
8.04 %
14
4.90 %
0
0.00 %
SER-FLEXI
42
907
0.33 %
92
10.14 %
774
85.34 %
51
5.62 %
41
4.52 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.05 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-NEW-IN
2
313
0.11 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
3
160
0.06 %
5
3.13 %
148
92.50 %
10
6.25 %
7
4.38 %
0
0.00 %
PAR-PERSIS
31
2,279
0.82 %
139
6.10 %
2013
88.33 %
169
7.42 %
127
5.57 %
0
0.00 %
PAR-RAPID
49
124
0.04 %
26
20.97 %
94
75.81 %
6
4.84 %
4
3.23 %
0
0.00 %
SER-FLEXI
9
512
0.19 %
18
3.52 %
480
93.75 %
15
2.93 %
12
2.34 %
2
0.39 %
Gwent
PAR-HERD-S
11
887
0.32 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
2
24
0.01 %
5
20.83 %
19
79.17 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
18
1,245
0.45 %
65
5.22 %
1121
90.04 %
78
6.27 %
58
4.66 %
1
0.08 %
PAR-RAPID
26
78
0.03 %
18
23.08 %
53
67.95 %
7
8.97 %
6
7.69 %
1
1.28 %
SER-FLEXI
1
20
0.01 %
2
10.00 %
18
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
36
2,381
0.86 %
79
3.32 %
2241
94.12 %
107
4.49 %
59
2.48 %
3
0.13 %
PAR-NEW-IN
3
8
0.00 %
2
25.00 %
4
50.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-OTHER
17
1,223
0.44 %
57
4.66 %
1081
88.39 %
106
8.67 %
85
6.95 %
0
0.00 %
PAR-PERSIS
122
11,615
4.20 %
367
3.16 %
10663
91.80 %
724
6.23 %
580
4.99 %
5
0.04 %
PAR-RAPID
117
317
0.11 %
58
18.30 %
233
73.50 %
20
6.31 %
13
4.10 %
13
4.10 %
SER-FLEXI
42
1,001
0.36 %
63
6.29 %
900
89.91 %
53
5.29 %
37
3.70 %
1
0.10 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-HERD-S
24
502
0.18 %
54
10.76 %
423
84.26 %
30
5.98 %
21
4.18 %
4
0.80 %
PAR-NEW-IN
29
977
0.35 %
43
4.40 %
897
91.81 %
44
4.50 %
36
3.68 %
1
0.10 %
PAR-OTHER
1
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
38
2,040
0.74 %
79
3.87 %
1851
90.74 %
123
6.03 %
100
4.90 %
10
0.49 %
PAR-RAPID
59
168
0.06 %
23
13.69 %
108
64.29 %
13
7.74 %
12
7.14 %
25
14.88 %
SER-FLEXI
14
388
0.14 %
25
6.44 %
356
91.75 %
10
2.58 %
7
1.80 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.00 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.14 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.04 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.04 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-PERSIS
1
7
0.00 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
75
0.03 %
14
18.67 %
59
78.67 %
3
4.00 %
2
2.67 %
0
0.00 %
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.13 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
7
560
0.20 %
29
5.18 %
516
92.14 %
27
4.82 %
15
2.68 %
0
0.00 %
PAR-RAPID
11
22
0.01 %
3
13.64 %
18
81.82 %
1
4.55 %
1
4.55 %
0
0.00 %
SER-FLEXI
1
20
0.01 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.01 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
2
148
0.05 %
21
14.19 %
111
75.00 %
17
11.49 %
16
10.81 %
0
0.00 %
PAR-PERSIS
4
550
0.20 %
26
4.73 %
500
90.91 %
28
5.09 %
24
4.36 %
0
0.00 %
PAR-RAPID
4
12
0.00 %
6
50.00 %
6
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
27
0.01 %
2
7.41 %
21
77.78 %
4
14.81 %
4
14.81 %
0
0.00 %
NE Wales
PAR-NEW-IN
75
6,307
2.28 %
196
3.11 %
5760
91.33 %
399
6.33 %
296
4.69 %
55
0.87 %
PAR-OTHER
10
85
0.03 %
16
18.82 %
61
71.76 %
11
12.94 %
8
9.41 %
0
0.00 %
PAR-PERSIS
10
1,617
0.58 %
39
2.41 %
1528
94.50 %
87
5.38 %
59
3.65 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
15
179
0.06 %
9
5.03 %
164
91.62 %
6
3.35 %
6
3.35 %
0
0.00 %
SER-FLEXI
41
1,012
0.37 %
72
7.11 %
844
83.40 %
53
5.24 %
43
4.25 %
53
5.24 %
Powys
PAR-NEW-IN
11
1,059
0.38 %
27
2.55 %
964
91.03 %
76
7.18 %
68
6.42 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
9
42
0.02 %
5
11.90 %
37
88.10 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
59
0.02 %
10
xx-xxxx-xxxx %
48
81.36 %
2
3.39 %
1
1.69 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
165
0.06 %
10
6.06 %
144
87.27 %
13
7.88 %
11
6.67 %
0
0.00 %
S Wales
PAR-RAPID
2
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.00 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
230
0.08 %
15
6.52 %
199
86.52 %
22
9.57 %
16
6.96 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
16
805
0.29 %
22
2.73 %
741
92.05 %
62
7.70 %
45
5.59 %
1
0.12 %
PAR-OTHER
1
17
0.01 %
0
0.00 %
17
100.00 %
1
5.88 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
14
460
0.17 %
8
1.74 %
421
91.52 %
25
5.43 %
16
3.48 %
15
3.26 %
PAR-OTHER
2
18
0.01 %
1
5.56 %
15
83.33 %
3
16.67 %
2
11.11 %
0
0.00 %
PAR-PERSIS
2
429
0.16 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
89
0.03 %
4
4.49 %
82
92.13 %
4
4.49 %
3
3.37 %
0
0.00 %
Wales
1197
52791
19.09 %
2608
4.94 %
47556
90.08 %
3183
6.03 %
2436
4.61 %
207
0.39 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.01 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
366
0.13 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
5
757
0.27 %
10
1.32 %
708
93.53 %
36
4.76 %
29
3.83 %
10
1.32 %
Kirkcudbright
PAR-CUL-P
1
352
0.13 %
7
1.99 %
340
96.59 %
8
2.27 %
5
1.42 %
0
0.00 %
PAR-NEW-IN
5
416
0.15 %
55
13.22 %
343
82.45 %
20
4.81 %
18
4.33 %
0
0.00 %
PAR-PERSIS
1
338
0.12 %
2
0.59 %
329
97.34 %
7
2.07 %
7
2.07 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
420
0.15 %
11
2.62 %
381
90.71 %
36
8.57 %
28
6.67 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Scotland
21
2688
0.97 %
90
3.35 %
2484
92.41 %
127
4.72 %
104
3.87 %
10
0.37 %
Grand Total
Sum:
xx-xxxx-xxxx
11330
247331
19554
15224
2738
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol October 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
166
20284
99.8%
483
2.4%
18042
88.9%
1294
6.4%
1044
5.1%
715
3.5%
Serial/Extended
3
31
0.2%
0
0.0 %
29
93.5%
2
6.5%
2
6.5%
0
0.0 %
Total
169
20315
100.0%
483
2.4%
18071
89.0%
1296
6.4%
1046
5.1%
715
3.5%
Scotland
Parallel
5
471
100.0%
7
1.5%
435
92.4%
24
5.1%
19
4.0%
10
2.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
5
471
100.0%
7
1.5%
435
92.4%
24
5.1%
19
4.0%
10
2.1%
Wales
Parallel
111
5755
97.5%
244
4.2%
5266
91.5%
314
5.5%
224
3.9%
25
0.4%
Serial/Extended
13
149
2.5%
9
6.0%
136
91.3%
7
4.7%
4
2.7%
0
0.0 %
Total
124
5904
100.0%
253
4.3%
5402
91.5%
321
5.4%
228
3.9%
25
0.4%
GB
Parallel
282
26510
99.3%
734
2.8%
23743
89.6%
1632
6.2%
1287
4.9%
750
2.8%
Serial/Extended
16
180
0.7%
9
5.0%
165
91.7%
9
5.0%
6
3.3%
0
0.0 %
Total
298
26690
100.0%
743
2.8%
23908
89.6%
xx-xxxx-xxxx
6.1%
1293
4.8%
750
2.8%
xx-xxxx-xxxx
124.00
5.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.2%
8552
3.9% 195767
89.2%
16143
7.4%
12620
5.8%
2476
1.1%
Serial/Extended
31
1713
0.8%
80
4.7%
1524
89.0%
101
5.9%
64
3.7%
45
2.6%
Total
xx-xxxx-xxxx
100.0%
8632
3.9% 197291
89.2%
16244
7.3%
12684
5.7%
2521
1.1%
Scotland
Parallel
21
2688
100.0%
90
3.3%
2484
92.4%
127
4.7%
104
3.9%
10
0.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
21
2688
100.0%
90
3.3%
2484
92.4%
127
4.7%
104
3.9%
10
0.4%
Wales
Parallel
1037
48655
92.2%
2303
4.7%
43933
90.3%
2986
6.1%
2284
4.7%
151
0.3%
Serial/Extended
160
4136
7.8%
305
7.4%
3623
87.6%
197
4.8%
152
3.7%
56
1.4%
Total
1197
52791
100.0%
2608
4.9%
47556
90.1%
3183
6.0%
2436
4.6%
207
0.4%
GB
Parallel
xx-xxxx-xxxx
97.9% 10945
4.0% 242184
89.5%
19256
7.1%
15008
5.5%
2637
1.0%
Serial/Extended
191
5849
2.1%
385
6.6%
5147
88.0%
298
5.1%
216
3.7%
101
1.7%
Total
xx-xxxx-xxxx
100.0% 11330
4.1% 247331
89.4%
19554
7.1%
15224
5.5%
2738
1.0%
#######
#######
21.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
15
177
0.7%
9
5.1%
162
91.5%
9
5.1 %
6
3.4 %
0
Parallel - Herd or Group Slaughter
14
2536
9.5%
68
2.7%
2397
94.5%
106
4.2 %
68
2.7 %
3
0.1 %
Parallel New Infection
111
12721
47.7%
236
1.9%
11337
89.1%
765
6.0 %
633
5.0 %
519
4.1 %
Parallel - new infection cull area
53
5925
22.2%
149
2.5%
5239
88.4%
447
7.5 %
344
5.8 %
193
3.3 %
Parallel - Other
10
61
0.2%
15
24.6%
38
62.3%
9
14.8 %
8
13.1 %
0
Parallel Persistent Infection
37
3206
12.0%
157
4.9%
2879
89.8%
197
6.1 %
147
4.6 %
23
0.7 %
Parallel - persistent infection cull area
13
1760
6.6%
79
4.5%
1609
91.4%
91
5.2 %
72
4.1 %
0
Parallel - Rapid Testing Two-times IRs
44
301
1.1%
30
10.0%
244
81.1%
17
5.6 %
15
5.0 %
12
4.0 %
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Total
298
26690
100.0%
743
2.8%
23908
89.6%
xx-xxxx-xxxx
6.1 %
1293
4.8 %
750
2.8 %
Table 4a. Summaryof IFN Gamma results by submission reason (October 2019)
England - HRA
Extended Flexible IFN-GAMMA test
2
28
0.1%
0
0.0 %
26
92.9%
2
7.1 %
2
7.1 %
0
Parallel New Infection
2
14
0.1%
0
0.0 %
12
85.7%
2
14.3 %
2
14.3 %
0
Parallel - new infection cull area
53
5925
22.2%
149
2.5%
5239
88.4%
447
7.5 %
344
5.8 %
193
3.3 %
Parallel - Other
2
6
0.0%
0
0.0 %
3
50.0%
3
50.0 %
3
50.0 %
0
Parallel Persistent Infection
7
1055
4.0%
43
4.1%
945
89.6%
61
5.8 %
54
5.1 %
13
1.2 %
Parallel - persistent infection cull area
12
1408
5.3%
72
5.1%
1269
90.1%
83
5.9 %
67
4.8 %
0
England - HRA
78
8436
31.6%
264
3.1%
7494
88.8%
598
7.1 %
472
5.6 %
206
2.4 %
England - Edge
Parallel New Infection
78
10272
38.5%
187
1.8%
9137
89.0%
538
5.2 %
442
4.3 %
506
4.9 %
Parallel Persistent Infection
3
675
2.5%
25
3.7%
617
91.4%
39
5.8 %
30
4.4 %
3
0.4 %
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
England - Edge
82
10950
41.0%
212
1.9%
9757
89.1%
577
5.3 %
472
4.3 %
509
4.6 %
England - LRA
Parallel New Infection
8
794
3.0%
3
0.4%
699
88.0%
102
12.8 %
92
11.6 %
0
Parallel Persistent Infection
1
135
0.5%
4
3.0%
121
89.6%
19
14.1 %
10
7.4 %
0
England - LRA
9
929
3.5%
7
0.8%
820
88.3%
121
13.0 %
102
11.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
10
120
0.4%
9
7.5%
108
90.0%
6
5.0 %
3
2.5 %
0
Parallel - Herd or Group Slaughter
14
2536
9.5%
68
2.7%
2397
94.5%
106
4.2 %
68
2.7 %
3
0.1 %
Parallel New Infection
5
398
1.5%
25
6.3%
363
91.2%
12
3.0 %
10
2.5 %
0
Parallel - Other
5
31
0.1%
15
48.4%
15
48.4%
1
3.2 %
1
3.2 %
0
Parallel Persistent Infection
23
981
3.7%
64
6.5%
880
89.7%
44
4.5 %
30
3.1 %
7
0.7 %
Parallel - Rapid Testing Two-times IRs
37
149
0.6%
24
16.1%
103
69.1%
12
8.1 %
10
6.7 %
12
8.1 %
Wales - High TB
94
4215
15.8%
205
4.9%
3866
91.7%
181
4.3 %
122
2.9 %
22
0.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
29
0.1%
0
0.0 %
28
96.6%
1
3.4 %
1
3.4 %
0
Parallel New Infection
9
729
2.7%
16
2.2%
667
91.5%
55
7.5 %
43
5.9 %
3
0.4 %
Parallel - Other
2
7
0.0%
0
0.0 %
3
42.9%
4
57.1 %
4
57.1 %
0
Parallel Persistent Infection
3
360
1.3%
21
5.8%
316
87.8%
34
9.4 %
23
6.4 %
0
Parallel - Rapid Testing Two-times IRs
6
151
0.6%
6
4.0%
140
92.7%
5
3.3 %
5
3.3 %
0
Wales - Intermediate TB
23
1276
4.8%
43
3.4%
1154
90.4%
99
7.8 %
76
6.0 %
3
0.2 %
Wales - Low TB
Parallel New Infection
5
395
1.5%
5
1.3%
364
92.2%
40
10.1 %
30
7.6 %
0
Parallel - Other
1
17
0.1%
0
0.0 %
17
100.0%
1
5.9 %
0
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
7
413
1.5%
5
1.2%
382
92.5%
41
9.9 %
30
7.3 %
0
Scotland - Scotland
Parallel New Infection
4
119
0.4%
0
0.0 %
95
79.8%
16
13.4 %
14
11.8 %
10
8.4 %
Parallel - persistent infection cull area
1
352
1.3%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland - Scotland
5
471
1.8%
7
1.5%
435
92.4%
24
5.1 %
19
4.0 %
10
2.1 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
186
5820
2.1%
385
6.6%
5119
88.0%
297
5.1 %
215
3.7 %
101
1.7 %
Parallel - Herd or Group Slaughter
144
7342
2.7%
434
5.9%
6546
89.2%
475
6.5 %
352
4.8 %
11
0.1 %
Parallel New Infection
1090 126794
45.8%
4041
3.2%
114140
90.0%
9293
7.3 %
7322
5.8 %
1313
1.0 %
Parallel - new infection cull area
517
60714
22.0%
2768
4.6%
53966
88.9%
4430
7.3 %
3421
5.6 %
559
0.9 %
Parallel - Other
89
4174
1.5%
278
6.7%
3459
82.9%
485
11.6 %
395
9.5 %
42
1.0 %
Parallel Persistent Infection
470
52750
19.1%
2295
4.4%
47273
89.6%
3291
6.2 %
2528
4.8 %
666
1.3 %
Parallel - persistent infection cull area
92
17715
6.4%
907
5.1%
15869
89.6%
1204
6.8 %
932
5.3 %
7
0.0 %
Parallel - Rapid Testing Two-times IRs
372
1250
0.5%
222
17.8%
931
74.5%
78
6.2 %
58
4.6 %
39
3.1 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
11330
4.1%
247331
89.4%
19554
7.1 %
15224
5.5 %
2738
1.0 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
24
1634
0.6%
79
4.8%
1448
88.6%
97
5.9 %
62
3.8 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
26
2102
0.8%
139
6.6%
1755
83.5%
264
12.6 %
208
9.9 %
0
Parallel - new infection cull area
515
60416
21.8%
2747
4.5%
53708
88.9%
4409
7.3 %
3402
5.6 %
559
0.9 %
Parallel - Other
26
1667
0.6%
88
5.3%
1283
77.0%
301
18.1 %
254
15.2 %
42
2.5 %
Parallel Persistent Infection
135
22098
8.0%
1111
5.0%
19349
87.6%
1450
6.6 %
1098
5.0 %
541
2.4 %
Parallel - persistent infection cull area
91
17363
6.3%
900
5.2%
15529
89.4%
1196
6.9 %
927
5.3 %
7
0.0 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
811 103112
37.3%
3213
3.1%
92983
90.2%
7322
7.1 %
5741
5.6 %
1193
1.2 %
Parallel - new infection cull area
2
298
0.1%
21
7.0%
258
86.6%
21
7.0 %
19
6.4 %
0
Parallel Persistent Infection
18
2026
0.7%
54
2.7%
1879
92.7%
114
5.6 %
90
4.4 %
3
0.1 %
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
79
9364
3.4%
264
2.8%
8274
88.4%
953
10.2 %
788
8.4 %
38
0.4 %
Parallel Persistent Infection
7
893
0.3%
7
0.8%
704
78.8%
108
12.1 %
89
10.0 %
93
10.4 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
England
xx-xxxx-xxxx
79.9%
8632
3.9%
197291
89.2%
16244
7.3 %
12684
5.7 %
2521
1.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
111
2929
1.1%
205
7.0%
2620
89.5%
134
4.6 %
101
3.4 %
3
0.1 %
Parallel - Herd or Group Slaughter
132
7184
2.6%
409
5.7%
6429
89.5%
457
6.4 %
336
4.7 %
11
0.2 %
Parallel New Infection
37
1419
0.5%
81
5.7%
1280
90.2%
67
4.7 %
57
4.0 %
1
0.1 %
Parallel - Other
40
1886
0.7%
138
7.3%
xx-xxxx-xxxx
87.3%
136
7.2 %
102
5.4 %
0
Parallel Persistent Infection
285
24009
8.7%
1005
4.2%
21860
91.0%
1431
6.0 %
1117
4.7 %
29
0.1 %
Parallel - Rapid Testing Two-times IRs
323
981
0.4%
197
20.1%
695
70.8%
70
7.1 %
50
5.1 %
39
4.0 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
47
1118
0.4%
96
8.6%
921
82.4%
59
5.3 %
48
4.3 %
53
4.7 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
88
7534
2.7%
233
3.1%
6871
91.2%
488
6.5 %
375
5.0 %
55
0.7 %
Parallel - Other
20
586
0.2%
51
8.7%
498
85.0%
44
7.5 %
37
6.3 %
0
Parallel Persistent Infection
22
2957
1.1%
109
3.7%
2743
92.8%
164
5.5 %
114
3.9 %
0
Parallel - Rapid Testing Two-times IRs
45
264
0.1%
25
9.5%
231
87.5%
8
3.0 %
8
3.0 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
30
1265
0.5%
30
2.4%
1162
91.9%
87
6.9 %
61
4.8 %
16
1.3 %
Parallel - Other
3
35
0.0%
1
2.9%
32
91.4%
4
11.4 %
2
5.7 %
0
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
4
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Wales
1197
52791
19.1%
2608
4.9%
47556
90.1%
3183
6.0 %
2436
4.6 %
207
0.4 %
Scotland - Scotland
Parallel New Infection
19
1998
0.7%
81
4.1%
1815
90.8%
112
5.6 %
92
4.6 %
10
0.5 %
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Parallel - persistent infection cull area
1
352
0.1%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland
21
2688
1.0%
90
3.3%
2484
92.4%
127
4.7 %
104
3.9 %
10
0.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 November
282
24570
192
1580
2
4
0
0
0
0
42
5
0
8
2
1336
0
169
0
10
December
228
21554
147
1241
6
8
0
0
0
0
92
2
0
7
1
1037
0
82
0
5
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
0
0
4
1
773
0
73
0
0
July
316
32549
201
1219
1
13
0
0
0
0
108
0
1
12
2
1020
1
58
0
1
August
262
21887
126
724
1
8
0
0
0
0
84
0
0
5
2
570
6
46
0
0
September
245
25317
134
693
1
15
0
0
0
0
95
0
2
1
0
534
45
0
0
0
October
298
26690
154
743
85
222
0
0
0
0
28
0
2
0
0
312
31
0
0
0
Totals (last
12 months)
3475
322712
2045
14151
109
305
0
0
0
0
1116
18
5
63
8
11600
89
734
2
23
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2028
254739
1313
10534
221
0
0
0
0
954
12
2
44
5
9090
8
97
0
2
Scotland
21
2688
11
90
0
0
0
0
0
12
0
0
2
0
70
0
5
0
0
Wales
1426
65285
721
3527
84
0
0
0
0
150
6
3
17
3
2440
81
632
2
21
Totals
3475
322712
2045
14151
305
0
0
0
0
1116
18
5
63
8
11600
89
734
2
23
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
Wiltshire
Warks
Somerset excl N
S Glamorgan
Pembs
Northumb
NE Wales
Kirkcudbright
Hampshire
Gwent
Dumfries & G
Devon
Cumbria
Cheshire
Carms
Bucks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during October 2019 (% submission per
County)
County
%Resampled %Retested
Bucks
2.30 %
3.07 %
Cambs
27.27 %
27.27 %
Carms
4.16 %
5.89 %
Ceredigion
7.02 %
8.77 %
Cheshire
3.20 %
3.77 %
Corn & Scilly
3.34 %
3.65 %
Cumbria
11.15 %
14.18 %
Derbyshire
4.70 %
5.99 %
Devon
6.44 %
8.78 %
Dorset
6.12 %
7.71 %
Dumfries & G
9.65 %
11.40 %
Gloucs
3.01 %
3.71 %
Gwent
3.11 %
4.35 %
Gwynedd
7.26 %
9.93 %
Hampshire
7.14 %
7.14 %
Heref
4.12 %
5.76 %
Kirkcudbright
2.24 %
3.08 %
Leics & Rut
2.36 %
3.24 %
NE Wales
6.18 %
8.46 %
Northants
2.15 %
3.23 %
Northumb
3.30 %
3.30 %
Oxon
8.21 %
10.02 %
Pembs
1.99 %
3.40 %
Powys
3.53 %
4.51 %
S Glamorgan
Shropshire
8.24 %
9.41 %
Somerset excl
6.94 %
6.94 %
Suffolk
12.42 %
13.42 %
Warks
4.07 %
5.20 %
W Glamorgan
6.96 %
9.57 %
Wiltshire
5.55 %
6.51 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19686
483
2.5%
20315
483
2.4%
Wales
5803
253
4.4%
5904
253
4.3%
Scotland
447
7
1.6%
471
7
1.5%
GB
25936
743
2.9%
26690
743
2.8%
Table 6a (i): Number of animals and submissions tested in October 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
195986
8632
4.4%
221109
8632
3.9%
Wales
48299
2608
5.4%
52791
2608
4.9%
Scotland
2010
90
4.5%
2688
90
3.3%
GB
246291
11330
4.6%
276588
11330
4.1%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19127
1054
5.5%
19262
1054
5.5%
Wales
4632
362
7.8%
4699
363
7.7%
Scotland
14
0
0.0 %
15
0.0 %
GB
23773
1416
6.0%
23976
1417
5.9%
Table 6a (ii): Number of animals and submissions tested in October 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
140798
7718
5.5%
160008
7718
4.8%
Wales
39468
1949
4.9%
45113
1950
4.3%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
182329
9848
5.4%
207362
9849
4.7%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
1
1
0
0
PRV-SP
2
2
0
0
Northern Somerset & South Glou
PRV-SP
1
1
0
0
Somerset excl North
PRV-SP
2
5
0
0
Total
6
9
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples October 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
2
7
0
0
PRV-SP
3
3
0
0
Dorset
PRV-SP
1
2
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SP
1
1
0
0
Somerset excl North
PRV-SE
6
247
16
6.48 %
PRV-SP
5
8
0
0
Staffordshire
PRV-SE
1
64
3
4.69 %
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
23
346
19
5.49 %
Isle of Man
SER-IOM
26
368
2
0.54 %
Isle of Man
SER-IOM
1
4
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
2
57
13
22.8%
North East Wales
3
38
2
5.3%
Pembrokeshire
3
72
3
4.2%
Powys
2
54
6
11.1%
TC0077
Sum:
221
24
10.9 %
Table 8a Number of Cattle Antibody Tests October 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
15
563
52
9.2%
Ceredigion
3
372
17
4.6%
North East Wales
27
896
116
12.9%
Pembrokeshire
19
618
35
5.7%
Powys
8
367
24
6.5%
South Wales
5
196
16
8.2%
Wiltshire
1
248
9
3.6%
TC0077
Sum:
3,260
269
8.3 %
Table 8b Number of Cattle Antibody Tests 2019