Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - September 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 157
Report Period 1st - 30th September 2019
Issued 10 October 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
September
2019
%
Total 2019
%
Submissions
245
2667
Samples
Total (%)
25317
100.00 %
249898
100.00 %
England
20801
82.16 %
200794
80.35 %
Wales
4509
17.81 %
46887
18.76 %
Scotland
7
0.03 %
2217
0.89 %
Parallel Tests
PAR-CUL-N
7695
30.39 %
54789
21.92 %
PAR-CUL-P
1983
7.83 %
15955
6.38 %
PAR-HERD-S
371
1.47 %
4806
1.92 %
PAR-NEW-IN
10590
41.83 %
114073
45.65 %
PAR-OTHER
154
0.61 %
4113
1.65 %
PAR-PERSIS
4220
16.67 %
49544
19.83 %
PAR-RAPID
63
0.25 %
949
0.38 %
Total(% of all samples)
25076
99.05 %
244229
97.73 %
Serial/Extended Tests
SER-FLEXI
241
0.95 %
5643
2.26 %
SER-FRAUD
0
0.00 %
18
0.01 %
SER-NSR
0
0.00 %
8
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
241
0.95 %
5669
2.27 %
Total
25317
100.00 %
249898
100.00 %
Retests
Total (% of all samples)
1972
7.79 %
17913
7.17 %
England
1739
6.87 %
14948
5.98 %
Wales
230
0.91 %
2862
1.15 %
Scotland
3
0.01 %
103
0.04 %
Resamples
Total (% of all samples)
1456
5.75 %
13931
5.57 %
England
1282
5.06 %
11638
4.66 %
Wales
171
0.68 %
2208
0.88 %
Scotland
3
0.01 %
85
0.03 %
Rejects
Total (% of all samples)
5
0.02 %
1988
0.80 %
England
4
0.02 %
1806
0.72 %
Wales
1
0.00 %
182
0.07 %
Scotland
0
0.00 %
Table 1. Monthly Totals for September 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
7695
30.4 %
PAR-CUL-P
1983
7.8 %
PAR-HERD-S
371
1.5 %
PAR-NEW-IN
10590
41.8 %
PAR-OTHER
154
0.6 %
PAR-PERSIS
4220
16.7 %
PAR-RAPID
63
0.2 %
SER-FLEXI
241
1.0 %
Total
25317
100.00 %
Figure 1. Number of samples submitted in each category during September 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Kirkcudbright
Powys
Pembs
Pembrokeshire
NE Wales
Gwynedd
Ceredigion
Carms
Carmarthenshire
Worcs
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Lincs
Leics & Rut
Heref
Hampshire
Gr London
Gloucs
Gloucestershire
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during September 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
Pembrokeshire
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Carmarthenshire
W Yorks
W Sussex
Worcs
Wiltshire
Warks
S Yorks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Lincs
Leics & Rut
Leicestershire and Rutland
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gr London
Gloucs
Gloucestershire
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
cotland
Wales
ngland
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
3
161
0.64 %
12
7.45 %
138
85.71 %
15
9.32 %
11
6.83 %
0
0.00 %
PAR-NEW-IN
3
438
1.73 %
3
0.68 %
412
94.06 %
33
7.53 %
23
5.25 %
0
0.00 %
PAR-PERSIS
2
306
1.21 %
51
16.67 %
236
77.12 %
22
7.19 %
19
6.21 %
0
0.00 %
Devon
PAR-CUL-N
15
3,082
12.17 %
60
1.95 %
2811
91.21 %
271
8.79 %
211
6.85 %
0
0.00 %
PAR-CUL-P
5
1,508
5.96 %
23
1.53 %
1434
95.09 %
70
4.64 %
51
3.38 %
0
0.00 %
Dorset
PAR-CUL-N
9
1,794
7.09 %
77
4.29 %
1599
89.13 %
159
8.86 %
118
6.58 %
0
0.00 %
PAR-NEW-IN
1
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Gloucestershire
PAR-CUL-N
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gloucs
PAR-CUL-N
5
311
1.23 %
10
3.22 %
266
85.53 %
39
12.54 %
35
11.25 %
0
0.00 %
PAR-CUL-P
2
83
0.33 %
6
7.23 %
75
90.36 %
8
9.64 %
2
2.41 %
0
0.00 %
Heref
PAR-PERSIS
4
110
0.43 %
10
9.09 %
92
83.64 %
10
9.09 %
8
7.27 %
0
0.00 %
Shropshire
PAR-OTHER
2
105
0.41 %
5
4.76 %
95
90.48 %
12
11.43 %
5
4.76 %
0
0.00 %
Somerset excl N
PAR-CUL-N
9
1,338
5.28 %
44
3.29 %
1225
91.55 %
137
10.24 %
69
5.16 %
0
0.00 %
Staffs
PAR-OTHER
1
12
0.05 %
0
0.00 %
12
100.00 %
1
8.33 %
0
0.00 %
0
0.00 %
Wiltshire
PAR-CUL-N
8
966
3.82 %
31
3.21 %
832
86.13 %
113
11.70 %
101
10.46 %
2
0.21 %
PAR-CUL-P
4
392
1.55 %
21
5.36 %
352
89.80 %
28
7.14 %
19
4.85 %
0
0.00 %
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
843
3.33 %
57
6.76 %
767
90.98 %
37
4.39 %
19
2.25 %
0
0.00 %
Worcs
PAR-PERSIS
1
41
0.16 %
0
0.00 %
41
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
2
180
0.71 %
1
0.56 %
169
93.89 %
11
6.11 %
10
5.56 %
0
0.00 %
Cheshire
PAR-CUL-N
1
38
0.15 %
2
5.26 %
29
76.32 %
9
23.68 %
7
18.42 %
0
0.00 %
PAR-NEW-IN
25
5,443
21.50 %
66
1.21 %
5104
93.77 %
382
7.02 %
273
5.02 %
0
0.00 %
Derbyshire
PAR-NEW-IN
8
1,078
4.26 %
32
2.97 %
965
89.52 %
105
9.74 %
81
7.51 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
276
1.09 %
2
0.72 %
247
89.49 %
34
12.32 %
27
9.78 %
0
0.00 %
PAR-PERSIS
1
37
0.15 %
2
5.41 %
28
75.68 %
9
24.32 %
7
18.92 %
0
0.00 %
Table 2a. Summary by County for September 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
1
4
0.02 %
0
0.00 %
4
100.00 %
1
25.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
13
1,260
4.98 %
35
2.78 %
1143
90.71 %
109
8.65 %
82
6.51 %
0
0.00 %
Warks
PAR-NEW-IN
8
554
2.19 %
9
1.62 %
519
93.68 %
29
5.23 %
24
4.33 %
2
0.36 %
England - LRA
Cumbria
PAR-NEW-IN
1
83
0.33 %
0
0.00 %
78
93.98 %
15
18.07 %
5
6.02 %
0
0.00 %
Gr London
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
1
50.00 %
2
100.00 %
1
50.00 %
0
0.00 %
Lincs
PAR-NEW-IN
3
320
1.26 %
4
1.25 %
244
76.25 %
76
23.75 %
72
22.50 %
0
0.00 %
N Yorks
PAR-PERSIS
1
22
0.09 %
0
0.00 %
22
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
147
20801
82.16 %
563
2.71 %
18952
91.11 %
1739
8.36 %
1282
6.16 %
4
0.02 %
Wales - High TB
Carmarthenshire
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Carms
PAR-HERD-S
4
78
0.31 %
10
12.82 %
64
82.05 %
5
6.41 %
4
5.13 %
0
0.00 %
PAR-OTHER
1
10
0.04 %
0
0.00 %
8
80.00 %
2
20.00 %
2
20.00 %
0
0.00 %
PAR-PERSIS
5
449
1.77 %
23
5.12 %
414
92.20 %
18
4.01 %
12
2.67 %
0
0.00 %
PAR-RAPID
2
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
5
350
1.38 %
6
1.71 %
323
92.29 %
27
7.71 %
21
6.00 %
0
0.00 %
PAR-RAPID
5
12
0.05 %
0
0.00 %
12
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
18
0.07 %
0
0.00 %
17
94.44 %
2
11.11 %
1
5.56 %
0
0.00 %
Pembrokeshire
PAR-RAPID
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
1
8
0.03 %
0
0.00 %
8
100.00 %
2
25.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
5
0.02 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
14
869
3.43 %
25
2.88 %
807
92.87 %
44
5.06 %
36
4.14 %
1
0.12 %
PAR-RAPID
9
26
0.10 %
6
23.08 %
20
76.92 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
63
0.25 %
2
3.17 %
59
93.65 %
3
4.76 %
2
3.17 %
0
0.00 %
Powys
PAR-HERD-S
6
285
1.13 %
4
1.40 %
271
95.09 %
17
5.96 %
10
3.51 %
0
0.00 %
PAR-NEW-IN
3
12
0.05 %
0
0.00 %
12
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
177
0.70 %
4
2.26 %
168
94.92 %
5
2.82 %
5
2.82 %
0
0.00 %
PAR-RAPID
3
11
0.04 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
NE Wales
PAR-NEW-IN
4
787
3.11 %
18
2.29 %
744
94.54 %
38
4.83 %
25
3.18 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
4
20
0.08 %
0
0.00 %
18
90.00 %
3
15.00 %
2
10.00 %
0
0.00 %
PAR-PERSIS
5
1,016
4.01 %
14
1.38 %
973
95.77 %
45
4.43 %
38
3.74 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
158
0.62 %
7
4.43 %
146
92.41 %
7
4.43 %
5
3.16 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
138
0.55 %
7
5.07 %
123
89.13 %
12
8.70 %
8
5.80 %
0
0.00 %
NE Wales
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
97
4509
17.81 %
130
2.88 %
4216
93.50 %
230
5.10 %
171
3.79 %
1
0.02 %
Scotland - Scotland
Kirkcudbright
PAR-NEW-IN
1
7
0.03 %
0
0.00 %
4
57.14 %
3
42.86 %
3
42.86 %
0
0.00 %
Scotland
1
7
0.03 %
0
0.00 %
4
57.14 %
3
42.86 %
3
42.86 %
0
0.00 %
Grand Total
Sum:
245
25317
693
23172
1972
1456
5
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
3
431
0.17 %
9
2.09 %
407
94.43 %
23
5.34 %
15
3.48 %
0
0.00 %
PAR-OTHER
4
83
0.03 %
1
1.20 %
70
84.34 %
12
14.46 %
12
14.46 %
0
0.00 %
PAR-PERSIS
9
1,490
0.60 %
45
3.02 %
1387
93.09 %
72
4.83 %
56
3.76 %
2
0.13 %
SER-FRAUD
2
18
0.01 %
0
0.00 %
17
94.44 %
1
5.56 %
1
5.56 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
41
4,206
1.68 %
139
3.30 %
3746
89.06 %
333
7.92 %
270
6.42 %
51
1.21 %
PAR-CUL-P
4
1,165
0.47 %
19
1.63 %
1035
88.84 %
132
11.33 %
109
9.36 %
2
0.17 %
PAR-NEW-IN
7
793
0.32 %
14
1.77 %
699
88.15 %
103
12.99 %
80
10.09 %
0
0.00 %
PAR-PERSIS
31
7,395
2.96 %
303
4.10 %
6481
87.64 %
420
5.68 %
316
4.27 %
295
3.99 %
SER-FLEXI
2
15
0.01 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
176
23,764
9.51 %
1214
5.11 %
21238
89.37 %
1593
6.70 %
1205
5.07 %
107
0.45 %
PAR-CUL-P
27
6,605
2.64 %
312
4.72 %
5932
89.81 %
446
6.75 %
357
5.40 %
4
0.06 %
PAR-NEW-IN
4
674
0.27 %
106
15.73 %
501
74.33 %
86
12.76 %
67
9.94 %
0
0.00 %
PAR-OTHER
7
532
0.21 %
6
1.13 %
352
66.17 %
202
37.97 %
174
32.71 %
0
0.00 %
PAR-PERSIS
19
2,346
0.94 %
162
6.91 %
2038
86.87 %
190
8.10 %
145
6.18 %
1
0.04 %
SER-FLEXI
8
849
0.34 %
50
5.89 %
732
86.22 %
47
5.54 %
22
2.59 %
45
5.30 %
Dorset
PAR-CUL-N
88
11,546
4.62 %
623
5.40 %
10249
88.77 %
804
6.96 %
618
5.35 %
56
0.49 %
PAR-CUL-P
13
2,896
1.16 %
234
8.08 %
2562
88.47 %
140
4.83 %
100
3.45 %
0
0.00 %
PAR-NEW-IN
3
41
0.02 %
1
2.44 %
31
75.61 %
9
21.95 %
9
21.95 %
0
0.00 %
SER-FLEXI
4
153
0.06 %
6
3.92 %
137
89.54 %
11
7.19 %
10
6.54 %
0
0.00 %
Gloucestershire
PAR-CUL-N
1
5
0.00 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
112
0.04 %
3
2.68 %
103
91.96 %
7
6.25 %
6
5.36 %
0
0.00 %
Gloucs
PAR-CUL-N
48
3,307
1.32 %
129
3.90 %
2774
83.88 %
353
10.67 %
296
8.95 %
108
3.27 %
PAR-CUL-P
10
1,278
0.51 %
52
4.07 %
1115
87.25 %
140
10.95 %
111
8.69 %
0
0.00 %
PAR-HERD-S
4
33
0.01 %
0
0.00 %
31
93.94 %
3
9.09 %
2
6.06 %
0
0.00 %
SER-FLEXI
2
30
0.01 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
15
1,724
0.69 %
69
4.00 %
1541
89.39 %
129
7.48 %
103
5.97 %
11
0.64 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
2
182
0.07 %
4
2.20 %
159
87.36 %
22
12.09 %
19
10.44 %
0
0.00 %
PAR-NEW-IN
1
11
0.00 %
0
0.00 %
10
90.91 %
4
36.36 %
1
9.09 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
29
3,406
1.36 %
138
4.05 %
3062
89.90 %
252
7.40 %
200
5.87 %
6
0.18 %
Shropshire
PAR-CUL-P
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
630
0.25 %
43
6.83 %
543
86.19 %
57
9.05 %
43
6.83 %
1
0.16 %
PAR-PERSIS
10
935
0.37 %
26
2.78 %
809
86.52 %
111
11.87 %
99
10.59 %
1
0.11 %
SER-FLEXI
5
557
0.22 %
21
3.77 %
509
91.38 %
34
6.10 %
27
4.85 %
0
0.00 %
Somerset excl N
PAR-CUL-N
38
4,504
1.80 %
205
4.55 %
4090
90.81 %
327
7.26 %
207
4.60 %
2
0.04 %
PAR-OTHER
2
238
0.10 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
13
1,907
0.76 %
110
5.77 %
1510
79.18 %
105
5.51 %
69
3.62 %
218
11.43 %
Staffs
PAR-OTHER
2
140
0.06 %
3
2.14 %
125
89.29 %
15
10.71 %
12
8.57 %
0
0.00 %
PAR-PERSIS
7
1,492
0.60 %
202
13.54 %
1201
80.50 %
130
8.71 %
90
6.03 %
0
0.00 %
Wiltshire
PAR-CUL-N
55
5,435
2.17 %
219
4.03 %
4826
88.79 %
423
7.78 %
359
6.61 %
31
0.57 %
PAR-CUL-P
19
3,389
1.36 %
197
5.81 %
3042
89.76 %
210
6.20 %
149
4.40 %
1
0.03 %
PAR-NEW-IN
7
456
0.18 %
15
3.29 %
398
87.28 %
53
11.62 %
43
9.43 %
0
0.00 %
PAR-OTHER
2
37
0.01 %
1
2.70 %
34
91.89 %
4
10.81 %
2
5.41 %
0
0.00 %
PAR-PERSIS
9
2,031
0.81 %
82
4.04 %
1875
92.32 %
109
5.37 %
69
3.40 %
5
0.25 %
SER-FLEXI
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Worcs
PAR-PERSIS
1
41
0.02 %
0
0.00 %
41
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
39
4,468
1.79 %
155
3.47 %
4016
89.88 %
362
8.10 %
297
6.65 %
0
0.00 %
PAR-PERSIS
3
204
0.08 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
25
2,403
0.96 %
85
3.54 %
2100
87.39 %
249
10.36 %
203
8.45 %
15
0.62 %
Cheshire
PAR-CUL-N
2
298
0.12 %
21
7.05 %
258
86.58 %
21
7.05 %
19
6.38 %
0
0.00 %
PAR-NEW-IN
185
37,630
15.06 %
876
2.33 %
34902
92.75 %
2338
6.21 %
1701
4.52 %
151
0.40 %
PAR-PERSIS
5
540
0.22 %
3
0.56 %
512
94.81 %
29
5.37 %
25
4.63 %
0
0.00 %
Derbyshire
PAR-NEW-IN
135
15,034
6.02 %
508
3.38 %
13264
88.23 %
1258
8.37 %
1049
6.98 %
213
1.42 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
E Sussex
PAR-NEW-IN
21
2,721
1.09 %
125
4.59 %
2450
90.04 %
157
5.77 %
149
5.48 %
13
0.48 %
Hampshire
PAR-NEW-IN
33
2,954
1.18 %
70
2.37 %
2675
90.56 %
265
8.97 %
211
7.14 %
0
0.00 %
PAR-PERSIS
1
37
0.01 %
2
5.41 %
28
75.68 %
9
24.32 %
7
18.92 %
0
0.00 %
SER-NSR
2
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leicestershire and
Rutland
PAR-NEW-IN
1
24
0.01 %
0
0.00 %
24
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
53
6,254
2.50 %
261
4.17 %
5548
88.71 %
434
6.94 %
350
5.60 %
95
1.52 %
Northants
PAR-NEW-IN
27
1,888
0.76 %
117
6.20 %
xx-xxxx-xxxx
89.41 %
129
6.83 %
83
4.40 %
0
0.00 %
Notts
PAR-NEW-IN
7
774
0.31 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
120
11,160
4.47 %
463
4.15 %
9893
88.65 %
961
8.61 %
754
6.76 %
50
0.45 %
Warks
PAR-NEW-IN
87
7,530
3.01 %
330
4.38 %
6668
88.55 %
562
7.46 %
446
5.92 %
86
1.14 %
PAR-PERSIS
6
570
0.23 %
19
3.33 %
526
92.28 %
32
5.61 %
25
4.39 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
6
398
0.16 %
22
5.53 %
347
87.19 %
27
6.78 %
21
5.28 %
8
2.01 %
Cumbria
PAR-NEW-IN
17
3,389
1.36 %
110
3.25 %
3085
91.03 %
269
7.94 %
194
5.72 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.04 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr London
PAR-NEW-IN
2
45
0.02 %
0
0.00 %
42
93.33 %
6
13.33 %
3
6.67 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
79
0.03 %
6
7.59 %
52
65.82 %
25
31.65 %
21
26.58 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.40 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
4
641
0.26 %
16
2.50 %
562
87.68 %
69
10.76 %
62
9.67 %
1
0.16 %
Kent
PAR-NEW-IN
1
10
0.00 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
106
0.04 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
11
1,074
0.43 %
24
2.23 %
937
87.24 %
122
11.36 %
113
10.52 %
0
0.00 %
Northumb
PAR-NEW-IN
2
205
0.08 %
0
0.00 %
153
74.63 %
60
29.27 %
52
25.37 %
0
0.00 %
PAR-PERSIS
3
183
0.07 %
1
0.55 %
32
17.49 %
58
31.69 %
57
31.15 %
93
50.82 %
N Yorks
PAR-NEW-IN
7
875
0.35 %
22
2.51 %
766
87.54 %
71
8.11 %
59
6.74 %
28
3.20 %
PAR-PERSIS
3
575
0.23 %
2
0.35 %
551
95.83 %
31
5.39 %
22
3.83 %
0
0.00 %
SER-FLEXI
2
50
0.02 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
S Yorks
PAR-NEW-IN
5
404
0.16 %
7
1.73 %
381
94.31 %
21
5.20 %
15
3.71 %
1
0.25 %
W Sussex
PAR-NEW-IN
2
239
0.10 %
2
0.84 %
163
68.20 %
81
33.89 %
74
30.96 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Yorks
PAR-HERD-S
2
24
0.01 %
8
33.33 %
14
58.33 %
2
8.33 %
2
8.33 %
0
0.00 %
England
1578
200794
80.35 %
8149
4.06 %
179220
89.26 %
14948
7.44 %
11638
5.80 %
1806
0.90 %
Wales - High TB
Carmarthenshire
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Carms
PAR-HERD-S
52
2,572
1.03 %
176
6.84 %
2257
87.75 %
174
6.77 %
137
5.33 %
2
0.08 %
PAR-NEW-IN
3
121
0.05 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
14
447
0.18 %
58
12.98 %
380
85.01 %
19
4.25 %
9
2.01 %
0
0.00 %
PAR-PERSIS
64
6,268
2.51 %
322
5.14 %
5697
90.89 %
321
5.12 %
238
3.80 %
13
0.21 %
PAR-RAPID
61
257
0.10 %
67
26.07 %
176
68.48 %
23
8.95 %
14
5.45 %
0
0.00 %
SER-FLEXI
37
836
0.33 %
87
10.41 %
709
84.81 %
49
5.86 %
40
4.78 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.06 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-NEW-IN
2
313
0.13 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
3
160
0.06 %
5
3.13 %
148
92.50 %
10
6.25 %
7
4.38 %
0
0.00 %
PAR-PERSIS
27
2,250
0.90 %
135
6.00 %
1992
88.53 %
164
7.29 %
123
5.47 %
0
0.00 %
PAR-RAPID
40
104
0.04 %
22
21.15 %
78
75.00 %
6
5.77 %
4
3.85 %
0
0.00 %
SER-FLEXI
8
511
0.20 %
18
3.52 %
479
93.74 %
15
2.94 %
12
2.35 %
2
0.39 %
Gwent
PAR-HERD-S
11
887
0.35 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
1
15
0.01 %
3
20.00 %
12
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
16
1,102
0.44 %
57
5.17 %
989
89.75 %
73
6.62 %
55
4.99 %
1
0.09 %
PAR-RAPID
24
69
0.03 %
15
21.74 %
49
71.01 %
5
7.25 %
4
5.80 %
1
1.45 %
SER-FLEXI
1
20
0.01 %
2
10.00 %
18
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembrokeshire
PAR-RAPID
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
26
437
0.17 %
43
9.84 %
374
85.58 %
43
9.84 %
21
4.81 %
0
0.00 %
PAR-NEW-IN
3
8
0.00 %
2
25.00 %
4
50.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-OTHER
15
1,218
0.49 %
56
4.60 %
1077
88.42 %
106
8.70 %
85
6.98 %
0
0.00 %
PAR-PERSIS
119
11,568
4.63 %
362
3.13 %
10622
91.82 %
721
6.23 %
579
5.01 %
5
0.04 %
PAR-RAPID
101
255
0.10 %
49
19.22 %
194
76.08 %
17
6.67 %
11
4.31 %
1
0.39 %
SER-FLEXI
41
999
0.40 %
63
6.31 %
898
89.89 %
53
5.31 %
37
3.70 %
1
0.10 %
Powys
PAR-HERD-S
23
488
0.20 %
53
10.86 %
410
84.02 %
30
6.15 %
21
4.30 %
4
0.82 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
24
579
0.23 %
18
3.11 %
534
92.23 %
32
5.53 %
26
4.49 %
1
0.17 %
PAR-OTHER
1
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
32
1,449
0.58 %
60
4.14 %
1304
89.99 %
98
6.76 %
82
5.66 %
3
0.21 %
PAR-RAPID
54
136
0.05 %
19
13.97 %
86
63.24 %
6
4.41 %
6
4.41 %
25
18.38 %
SER-FLEXI
11
342
0.14 %
21
6.14 %
316
92.40 %
6
1.75 %
5
1.46 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.00 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.15 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.04 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.05 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-PERSIS
1
7
0.00 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
75
0.03 %
14
18.67 %
59
78.67 %
3
4.00 %
2
2.67 %
0
0.00 %
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.14 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
7
560
0.22 %
29
5.18 %
516
92.14 %
27
4.82 %
15
2.68 %
0
0.00 %
PAR-RAPID
10
21
0.01 %
3
14.29 %
17
80.95 %
1
4.76 %
1
4.76 %
0
0.00 %
SER-FLEXI
1
20
0.01 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.01 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
2
148
0.06 %
21
14.19 %
111
75.00 %
17
11.49 %
16
10.81 %
0
0.00 %
PAR-PERSIS
4
550
0.22 %
26
4.73 %
500
90.91 %
28
5.09 %
24
4.36 %
0
0.00 %
PAR-RAPID
3
5
0.00 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
27
0.01 %
2
7.41 %
21
77.78 %
4
14.81 %
4
14.81 %
0
0.00 %
NE Wales
PAR-NEW-IN
68
5,993
2.40 %
192
3.20 %
5474
91.34 %
369
6.16 %
275
4.59 %
52
0.87 %
PAR-OTHER
8
78
0.03 %
16
20.51 %
58
74.36 %
7
8.97 %
4
5.13 %
0
0.00 %
PAR-PERSIS
8
1,487
0.60 %
33
2.22 %
1411
94.89 %
75
5.04 %
52
3.50 %
0
0.00 %
PAR-RAPID
13
44
0.02 %
8
18.18 %
35
79.55 %
1
2.27 %
1
2.27 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
38
983
0.39 %
72
7.32 %
816
83.01 %
52
5.29 %
42
4.27 %
53
5.39 %
Powys
PAR-NEW-IN
10
809
0.32 %
25
3.09 %
727
89.86 %
64
7.91 %
57
7.05 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
7
34
0.01 %
3
8.82 %
31
91.18 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
59
0.02 %
10
xx-xxxx-xxxx %
48
81.36 %
2
3.39 %
1
1.69 %
0
0.00 %
S Wales
PAR-RAPID
2
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.00 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
11
410
0.16 %
17
4.15 %
377
91.95 %
22
5.37 %
15
3.66 %
1
0.24 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
14
460
0.18 %
8
1.74 %
421
91.52 %
25
5.43 %
16
3.48 %
15
3.26 %
PAR-OTHER
2
18
0.01 %
1
5.56 %
15
83.33 %
3
16.67 %
2
11.11 %
0
0.00 %
PAR-PERSIS
2
429
0.17 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
89
0.04 %
4
4.49 %
82
92.13 %
4
4.49 %
3
3.37 %
0
0.00 %
Wales
1073
46887
18.76 %
2355
5.02 %
42154
89.91 %
2862
6.10 %
2208
4.71 %
182
0.39 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.02 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
366
0.15 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
2
643
0.26 %
10
1.56 %
615
95.65 %
23
3.58 %
18
2.80 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
4
411
0.16 %
55
13.38 %
341
82.97 %
17
4.14 %
15
3.65 %
0
0.00 %
PAR-PERSIS
1
338
0.14 %
2
0.59 %
329
97.34 %
7
2.07 %
7
2.07 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
420
0.17 %
11
2.62 %
381
90.71 %
36
8.57 %
28
6.67 %
0
0.00 %
Scotland
16
2217
0.89 %
83
3.74 %
2049
92.42 %
103
4.65 %
85
3.83 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
10587
223423
17913
13931
1988
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol September 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
147
20801
100.0%
563
2.7%
18952
91.1%
1739
8.4%
1282
6.2%
4
0.0%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
147
20801
100.0%
563
2.7%
18952
91.1%
1739
8.4%
1282
6.2%
4
0.0%
Scotland
Parallel
1
7
100.0%
0
0.0 %
4
57.1%
3
42.9%
3
42.9%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
7
100.0%
0
0.0 %
4
57.1%
3
42.9%
3
42.9%
0
0.0 %
Wales
Parallel
82
4268
94.7%
121
2.8%
3992
93.5%
218
5.1%
163
3.8%
1
0.0%
Serial/Extended
15
241
5.3%
9
3.7%
224
92.9%
12
5.0%
8
3.3%
0
0.0 %
Total
97
4509
100.0%
130
2.9%
4216
93.5%
230
5.1%
171
3.8%
1
0.0%
GB
Parallel
230
25076
99.0%
684
2.7%
22948
91.5%
1960
7.8%
1448
5.8%
5
0.0%
Serial/Extended
15
241
1.0%
9
3.7%
224
92.9%
12
5.0%
8
3.3%
0
0.0 %
Total
245
25317
100.0%
693
2.7%
23172
91.5%
1972
7.8%
1456
5.8%
5
0.0%
147.00
97.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.2%
8069
4.1% 177725
89.3%
14849
7.5%
11576
5.8%
1761
0.9%
Serial/Extended
28
xx-xxxx-xxxx
0.8%
80
4.8%
1495
88.9%
99
5.9%
62
3.7%
45
2.7%
Total
xx-xxxx-xxxx
100.0%
8149
4.1% 179220
89.3%
14948
7.4%
11638
5.8%
1806
0.9%
Scotland
Parallel
16
2217
100.0%
83
3.7%
2049
92.4%
103
4.6%
85
3.8%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
16
2217
100.0%
83
3.7%
2049
92.4%
103
4.6%
85
3.8%
0
0.0 %
Wales
Parallel
926
42900
91.5%
2059
4.8%
38667
90.1%
2672
6.2%
2060
4.8%
126
0.3%
Serial/Extended
147
3987
8.5%
296
7.4%
3487
87.5%
190
4.8%
148
3.7%
56
1.4%
Total
1073
46887
100.0%
2355
5.0%
42154
89.9%
2862
6.1%
2208
4.7%
182
0.4%
GB
Parallel
xx-xxxx-xxxx
97.7% 10211
4.2% 218441
89.4%
17624
7.2%
13721
5.6%
1887
0.8%
Serial/Extended
175
5669
2.3%
376
6.6%
4982
87.9%
289
5.1%
210
3.7%
101
1.8%
Total
xx-xxxx-xxxx
100.0% 10587
4.2% 223423
89.4%
17913
7.2%
13931
5.6%
1988
0.8%
#######
#######
16.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
15
241
1.0%
9
3.7%
224
92.9%
12
5.0 %
8
3.3 %
0
Parallel - Herd or Group Slaughter
11
371
1.5%
14
3.8%
343
92.5%
24
6.5 %
14
3.8 %
0
Parallel New Infection
80
10590
41.8%
177
1.7%
9776
92.3%
851
8.0 %
635
6.0 %
2
0.0 %
Parallel - new infection cull area
51
7695
30.4%
236
3.1%
6905
89.7%
743
9.7 %
552
7.2 %
2
0.0 %
Parallel - Other
10
154
0.6%
6
3.9%
138
89.6%
19
12.3 %
10
6.5 %
0
Parallel Persistent Infection
44
4220
16.7%
192
4.5%
3871
91.7%
217
5.1 %
165
3.9 %
1
0.0 %
Parallel - persistent infection cull area
11
1983
7.8%
50
2.5%
1861
93.8%
106
5.3 %
72
3.6 %
0
Parallel - Rapid Testing Two-times IRs
23
63
0.2%
9
14.3%
54
85.7%
0
0
0
Total
245
25317
100.0%
693
2.7%
23172
91.5%
1972
7.8 %
1456
5.8 %
5
0.0 %
Table 4a. Summaryof IFN Gamma results by submission reason (September 2019)
England - HRA
Parallel New Infection
5
445
1.8%
3
0.7%
418
93.9%
34
7.6 %
24
5.4 %
0
Parallel - new infection cull area
50
7657
30.2%
234
3.1%
6876
89.8%
734
9.6 %
545
7.1 %
2
0.0 %
Parallel - Other
4
119
0.5%
5
4.2%
108
90.8%
14
11.8 %
6
5.0 %
0
Parallel Persistent Infection
10
1300
5.1%
118
9.1%
1136
87.4%
69
5.3 %
46
3.5 %
0
Parallel - persistent infection cull area
11
1983
7.8%
50
2.5%
1861
93.8%
106
5.3 %
72
3.6 %
0
England - HRA
80
11504
45.4%
410
3.6%
10399
90.4%
957
8.3 %
693
6.0 %
2
0.0 %
England - Edge
Parallel New Infection
59
8795
34.7%
145
1.6%
8151
92.7%
671
7.6 %
497
5.7 %
2
0.0 %
Parallel - new infection cull area
1
38
0.2%
2
5.3%
29
76.3%
9
23.7 %
7
18.4 %
0
Parallel Persistent Infection
1
37
0.1%
2
5.4%
28
75.7%
9
24.3 %
7
18.9 %
0
England - Edge
61
8870
35.0%
149
1.7%
8208
92.5%
689
7.8 %
511
5.8 %
2
0.0 %
England - LRA
Parallel New Infection
5
405
1.6%
4
1.0%
323
79.8%
93
23.0 %
78
19.3 %
0
Parallel Persistent Infection
1
22
0.1%
0
0.0 %
22
100.0%
0
0
0
England - LRA
6
427
1.7%
4
0.9%
345
80.8%
93
21.8 %
78
18.3 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
8
83
0.3%
2
2.4%
78
94.0%
5
6.0 %
3
3.6 %
0
Parallel - Herd or Group Slaughter
11
371
1.5%
14
3.8%
343
92.5%
24
6.5 %
14
3.8 %
0
Parallel New Infection
3
12
0.0%
0
0.0 %
12
100.0%
0
0
0
Parallel - Other
2
15
0.1%
1
6.7%
12
80.0%
2
13.3 %
2
13.3 %
0
Parallel Persistent Infection
27
1845
7.3%
58
3.1%
1712
92.8%
94
5.1 %
74
4.0 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
21
59
0.2%
9
15.3%
50
84.7%
0
0
0
Wales - High TB
72
2385
9.4%
84
3.5%
2207
92.5%
125
5.2 %
93
3.9 %
1
0.0 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
158
0.6%
7
4.4%
146
92.4%
7
4.4 %
5
3.2 %
0
Parallel New Infection
4
787
3.1%
18
2.3%
744
94.5%
38
4.8 %
25
3.2 %
0
Parallel - Other
4
20
0.1%
0
0.0 %
18
90.0%
3
15.0 %
2
10.0 %
0
Parallel Persistent Infection
5
1016
4.0%
14
1.4%
973
95.8%
45
4.4 %
38
3.7 %
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Intermediate TB
21
1983
7.8%
39
2.0%
1883
95.0%
93
4.7 %
70
3.5 %
0
Wales - Low TB
Parallel New Infection
3
139
0.5%
7
5.0%
124
89.2%
12
8.6 %
8
5.8 %
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Low TB
4
141
0.6%
7
5.0%
126
89.4%
12
8.5 %
8
5.7 %
0
Scotland - Scotland
Parallel New Infection
1
7
0.0%
0
0.0 %
4
57.1%
3
42.9 %
3
42.9 %
0
Scotland - Scotland
1
7
0.0%
0
0.0 %
4
57.1%
3
42.9 %
3
42.9 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
171
5643
2.3%
376
6.7%
4957
87.8%
288
5.1 %
209
3.7 %
101
1.8 %
Parallel - Herd or Group Slaughter
130
4806
1.9%
366
7.6%
4149
86.3%
369
7.7 %
284
5.9 %
8
0.2 %
Parallel New Infection
979 114073
45.6%
3805
3.3%
102803
90.1%
8528
7.5 %
6689
5.9 %
794
0.7 %
Parallel - new infection cull area
464
54789
21.9%
2619
4.8%
48727
88.9%
3983
7.3 %
3077
5.6 %
366
0.7 %
Parallel - Other
79
4113
1.6%
263
6.4%
3421
83.2%
476
11.6 %
387
9.4 %
42
1.0 %
Parallel Persistent Infection
433
49544
19.8%
2138
4.3%
44394
89.6%
3094
6.2 %
2381
4.8 %
643
1.3 %
Parallel - persistent infection cull area
79
15955
6.4%
828
5.2%
14260
89.4%
1113
7.0 %
860
5.4 %
7
0.0 %
Parallel - Rapid Testing Two-times IRs
328
949
0.4%
192
20.2%
687
72.4%
61
6.4 %
43
4.5 %
27
2.8 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
10587
4.2%
223423
89.4%
17913
7.2 %
13931
5.6 %
1988
0.8 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
22
1606
0.6%
79
4.9%
1422
88.5%
95
5.9 %
60
3.7 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
24
2088
0.8%
139
6.7%
1743
83.5%
262
12.5 %
206
9.9 %
0
Parallel - new infection cull area
462
54491
21.8%
2598
4.8%
48469
88.9%
3962
7.3 %
3058
5.6 %
366
0.7 %
Parallel - Other
24
1661
0.7%
88
5.3%
1280
77.1%
298
17.9 %
251
15.1 %
42
2.5 %
Parallel Persistent Infection
128
21043
8.4%
1068
5.1%
18404
87.5%
1389
6.6 %
1044
5.0 %
528
2.5 %
Parallel - persistent infection cull area
79
15955
6.4%
828
5.2%
14260
89.4%
1113
7.0 %
860
5.4 %
7
0.0 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
733
92840
37.2%
3026
3.3%
83846
90.3%
6784
7.3 %
5299
5.7 %
687
0.7 %
Parallel - new infection cull area
2
298
0.1%
21
7.0%
258
86.6%
21
7.0 %
19
6.4 %
0
Parallel Persistent Infection
15
1351
0.5%
29
2.1%
1262
93.4%
75
5.6 %
60
4.4 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
71
8570
3.4%
261
3.0%
7575
88.4%
851
9.9 %
696
8.1 %
38
0.4 %
Parallel Persistent Infection
6
758
0.3%
3
0.4%
583
76.9%
89
11.7 %
79
10.4 %
93
12.3 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
England
xx-xxxx-xxxx
80.4%
8149
4.1%
179220
89.3%
14948
7.4 %
11638
5.8 %
1806
0.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
101
2809
1.1%
196
7.0%
2512
89.4%
128
4.6 %
98
3.5 %
3
0.1 %
Parallel - Herd or Group Slaughter
118
4648
1.9%
341
7.3%
4032
86.7%
351
7.6 %
268
5.8 %
8
0.2 %
Parallel New Infection
32
1021
0.4%
56
5.5%
917
89.8%
55
5.4 %
47
4.6 %
1
0.1 %
Parallel - Other
35
1855
0.7%
123
6.6%
1631
87.9%
135
7.3 %
101
5.4 %
0
Parallel Persistent Infection
262
23028
9.2%
941
4.1%
20980
91.1%
1387
6.0 %
1087
4.7 %
22
0.1 %
Parallel - Rapid Testing Two-times IRs
286
832
0.3%
173
20.8%
592
71.2%
58
7.0 %
40
4.8 %
27
3.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
44
1089
0.4%
96
8.8%
893
82.0%
58
5.3 %
47
4.3 %
53
4.9 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
79
6805
2.7%
217
3.2%
6204
91.2%
433
6.4 %
332
4.9 %
52
0.8 %
Parallel - Other
18
579
0.2%
51
8.8%
495
85.5%
40
6.9 %
33
5.7 %
0
Parallel Persistent Infection
19
2597
1.0%
88
3.4%
2427
93.5%
130
5.0 %
91
3.5 %
0
Parallel - Rapid Testing Two-times IRs
39
113
0.0%
19
16.8%
91
80.5%
3
2.7 %
3
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
25
870
0.3%
25
2.9%
798
91.7%
47
5.4 %
31
3.6 %
16
1.8 %
Parallel - Other
2
18
0.0%
1
5.6%
15
83.3%
3
16.7 %
2
11.1 %
0
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
3
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Wales
1073
46887
18.8%
2355
5.0%
42154
89.9%
2862
6.1 %
2208
4.7 %
182
0.4 %
Scotland - Scotland
Parallel New Infection
15
1879
0.8%
81
4.3%
1720
91.5%
96
5.1 %
78
4.2 %
0
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Scotland
16
2217
0.9%
83
3.7%
2049
92.4%
103
4.6 %
85
3.8 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 October
274
23976
152
1417
1
8
0
0
0
0
100
2
0
3
0
1242
0
44
0
2
November
282
24570
192
1580
2
4
0
0
0
0
42
5
0
8
2
1336
0
169
0
10
December
228
21554
147
1241
6
8
0
0
0
0
92
2
0
7
1
1037
0
82
0
5
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
0
0
4
1
773
0
73
0
0
July
316
32549
201
1219
1
26
0
0
0
0
108
0
2
11
2
1007
13
46
0
1
August
262
21887
126
724
3
8
0
0
0
0
87
0
2
2
0
570
52
0
0
0
September
245
25317
134
693
1
300
0
0
0
0
65
0
2
0
0
312
12
0
0
0
Totals (last
12 months)
3451
319998
2043
14825
27
389
0
0
0
0
1161
20
6
61
6
12295
83
720
2
25
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2016
253686
1319
11105
296
0
0
0
0
983
13
3
43
5
9619
8
96
0
2
Scotland
18
2232
10
83
0
0
0
0
0
12
0
0
2
0
63
1
4
0
0
Wales
1417
64080
714
3637
93
0
0
0
0
166
7
3
16
1
2613
74
620
2
23
Totals
3451
319998
2043
14825
389
0
0
0
0
1161
20
6
61
6
12295
83
720
2
25
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
160
Worcs
Warks
Somerset excl N
Powys
Pembrokeshire
N Yorks
Lincs
Kirkcudbright
Hampshire
Gr London
Gloucestershire
Devon
Cumbria
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during September 2019 (% submission per
County)
County
%Resampled %Retested
Avon
50.00 %
50.00 %
Berks
5.56 %
6.11 %
Carmarthenshi
Carms
3.30 %
4.58 %
Ceredigion
5.79 %
7.63 %
Cheshire
5.11 %
7.13 %
Corn & Scilly
5.86 %
7.73 %
Cumbria
6.02 %
18.07 %
Derbyshire
7.51 %
9.74 %
Devon
5.71 %
7.43 %
Dorset
6.61 %
8.89 %
Gloucestershire
Gloucs
9.39 %
11.93 %
Gr London
50.00 %
100.00 %
Gwynedd
5.80 %
8.70 %
Hampshire
10.86 %
13.74 %
Heref
7.27 %
9.09 %
Kirkcudbright
42.86 %
42.86 %
Leics & Rut
25.00 %
Lincs
22.50 %
23.75 %
NE Wales
3.52 %
4.68 %
N Yorks
Oxon
6.51 %
8.65 %
Pembrokeshire
Pembs
3.91 %
5.05 %
Powys
3.09 %
4.54 %
Shropshire
4.76 %
11.43 %
Somerset excl
5.16 %
10.24 %
Staffs
8.33 %
Warks
4.33 %
5.23 %
Wiltshire
6.31 %
8.08 %
Worcs

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20426
563
2.8%
20801
563
2.7%
Wales
4458
130
2.9%
4509
130
2.9%
Scotland
7
0
0.0 %
7
0.0 %
GB
24891
693
2.8%
25317
693
2.7%
Table 6a (i): Number of animals and submissions tested in September 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
181696
8149
4.5%
200794
8149
4.1%
Wales
42681
2355
5.5%
46887
2355
5.0%
Scotland
1889
83
4.4%
2217
83
3.7%
GB
226262
10587
4.7%
249898
10587
4.2%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of September
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
16078
665
4.1%
16273
665
4.1%
Wales
5072
281
5.5%
5168
281
5.4%
GB
21148
946
4.5%
21441
946
4.4%
Table 6a (ii): Number of animals and submissions tested in September 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of September
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
123818
6664
5.4%
140746
6664
4.7%
Wales
35833
1587
4.4%
40414
1587
3.9%
Scotland
2066
181
8.8%
2226
181
8.1%
GB
161700
8432
5.2%
183386
8432
4.6%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Somerset excl North
PRV-SE
2
206
1
0.49 %
Staffordshire
PRV-SE
1
64
3
4.69 %
Total
4
272
4
1.47 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples September 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
1
6
0
0
PRV-SP
1
1
0
0
Dorset
PRV-SP
1
2
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Somerset excl North
PRV-SE
6
247
16
6.48 %
PRV-SP
3
3
0
0
Staffordshire
PRV-SE
1
64
3
4.69 %
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
17
337
19
5.64 %
Isle of Man
SER-IOM
25
364
2
0.55 %
Isle of Man
SER-IOM
5
248
1
0.40 %

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
79
5
6.3%
North East Wales
5
113
9
8.0%
Pembrokeshire
2
81
5
6.2%
TC0077
Sum:
273
19
7.0 %
Table 8a Number of Cattle Antibody Tests September 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
13
506
39
7.7%
Ceredigion
3
372
17
4.6%
North East Wales
24
850
112
13.2%
Pembrokeshire
16
546
32
5.9%
Powys
6
313
18
5.8%
South Wales
5
196
16
8.2%
Wiltshire
1
248
9
3.6%
TC0077
Sum:
3,031
243
8.0 %
Table 8b Number of Cattle Antibody Tests 2019