Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - May 2023
Source: environment.data.gov.uk (2024-07-03)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2023. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period 1st - 31st May 2023
Issued 10 June 2023

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
May 2023
%
Total 2023
%
Submissions
208
1223
Samples
Total (%)
13834
100.00 %
91156
100.00 %
England
9134
66.03 %
58731
64.43 %
Wales
3966
28.67 %
26668
29.26 %
Scotland
734
5.31 %
5757
6.32 %
Parallel Tests
PAR-CHR
667
4.82 %
xx-xxxx-xxxx
1.86 %
PAR-CUL-N
254
1.84 %
2572
2.82 %
PAR-CUL-P
0
0.00 %
539
0.59 %
PAR-HERD-S
61
0.44 %
457
0.50 %
PAR-IR-EX
65
0.47 %
237
0.26 %
PAR-IR-SEV
341
2.46 %
1091
1.20 %
PAR-NEW-IN
3344
24.17 %
32824
36.01 %
PAR-OTHER
47
0.34 %
1523
1.67 %
PAR-PERSIS
2470
17.85 %
7360
8.07 %
PAR-POSTM
0
0.00 %
94
0.10 %
PAR-RAPID
50
0.36 %
350
0.38 %
PAR-RECUR
6243
45.13 %
38888
42.66 %
Total(% of all samples)
13542
97.89 %
87627
96.13 %
Serial/Extended Tests
SER-ANOM
2
0.01 %
2
0.00 %
SER-FLEXI
290
2.10 %
3513
3.85 %
SER-FRAUD
0
0.00 %
14
0.02 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
292
2.11 %
3529
3.87 %
Total
13834
100.00 %
91156
100.00 %
Retests
Total (% of all samples)
748
5.41 %
6824
7.49 %
England
458
3.31 %
4156
4.56 %
Wales
220
1.59 %
2110
2.31 %
Scotland
70
0.51 %
558
0.61 %
Resamples
Total (% of all samples)
586
4.24 %
5631
6.18 %
England
363
2.62 %
3541
3.88 %
Wales
161
1.16 %
1575
1.73 %
Scotland
62
0.45 %
515
0.56 %
Rejects
Total (% of all samples)
50
0.36 %
1585
1.74 %
England
5
0.04 %
1101
1.21 %
Wales
45
0.33 %
410
0.45 %
Scotland
0
0.00 %
74
0.08 %
Table 1. Monthly Totals for May 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-ANOM
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
667
4.8 %
PAR-CUL-N
254
1.8 %
PAR-HERD-S
61
0.4 %
PAR-IR-EX
65
0.5 %
PAR-IR-SEV
341
2.5 %
PAR-NEW-IN
3344
24.2 %
PAR-OTHER
47
0.3 %
PAR-PERSIS
2470
17.9 %
PAR-RAPID
50
0.4 %
PAR-RECUR
6243
45.1 %
SER-ANOM
2
0.0 %
SER-FLEXI
290
2.1 %
Total
13834
100.00 %
Figure 1. Number of samples submitted in each category during May 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
Kirkcudbright
Ayrshire
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Conwy
Ceredigion
Carms
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northants
Leics & Rut
Humberside
Heref
Gloucs
E Sussex
Devon
Derbyshire
Cumbria
Corn & Scilly
Bucks
Avon
Scotland
Wales
England
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during May 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
Wigtown
West lothian
Kirkcudbright
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NW Wales
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Conwy
Cheshire
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
4
533
3.85 %
10
1.88 %
505
94.75 %
21
3.94 %
17
3.19 %
1
0.19 %
Corn & Scilly
PAR-HERD-S
1
61
0.44 %
1
1.64 %
59
96.72 %
4
6.56 %
1
1.64 %
0
0.00 %
PAR-RECUR
11
1,372
9.92 %
25
1.82 %
1274
92.86 %
91
6.63 %
71
5.17 %
2
0.15 %
Devon
PAR-CUL-N
2
233
1.68 %
9
3.86 %
207
88.84 %
24
10.30 %
16
6.87 %
1
0.43 %
PAR-PERSIS
3
918
6.64 %
38
4.14 %
860
93.68 %
30
3.27 %
20
2.18 %
0
0.00 %
PAR-RECUR
9
1,002
7.24 %
43
4.29 %
923
92.12 %
46
4.59 %
36
3.59 %
0
0.00 %
Gloucs
PAR-CUL-N
1
16
0.12 %
2
12.50 %
14
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
Heref
PAR-RECUR
3
701
5.07 %
51
7.28 %
631
90.01 %
23
3.28 %
19
2.71 %
0
0.00 %
Shropshire
PAR-RECUR
1
424
3.06 %
14
3.30 %
388
91.51 %
25
5.90 %
22
5.19 %
0
0.00 %
Somerset excl N
PAR-CHR
2
142
1.03 %
22
15.49 %
112
78.87 %
12
8.45 %
8
5.63 %
0
0.00 %
PAR-RECUR
4
1,028
7.43 %
85
8.27 %
919
89.40 %
30
2.92 %
24
2.33 %
0
0.00 %
Staffs
PAR-CHR
3
525
3.79 %
15
2.86 %
486
92.57 %
28
5.33 %
24
4.57 %
0
0.00 %
PAR-RECUR
2
476
3.44 %
20
4.20 %
443
93.07 %
16
3.36 %
13
2.73 %
0
0.00 %
Wiltshire
PAR-CUL-N
1
5
0.04 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
240
1.73 %
0
0.00 %
227
94.58 %
15
6.25 %
13
5.42 %
0
0.00 %
England - Edge
Bucks
PAR-NEW-IN
3
237
1.71 %
6
2.53 %
218
91.98 %
16
6.75 %
13
5.49 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
68
0.49 %
3
4.41 %
64
94.12 %
1
1.47 %
1
1.47 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
240
1.73 %
3
1.25 %
216
90.00 %
24
10.00 %
21
8.75 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
1
4
0.03 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Northants
PAR-NEW-IN
3
245
1.77 %
2
0.82 %
225
91.84 %
20
8.16 %
17
6.94 %
1
0.41 %
Oxon
PAR-NEW-IN
1
57
0.41 %
0
0.00 %
56
98.25 %
2
3.51 %
1
1.75 %
0
0.00 %
PAR-RECUR
4
356
2.57 %
18
5.06 %
325
91.29 %
17
4.78 %
13
3.65 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
148
1.07 %
3
2.03 %
138
93.24 %
7
4.73 %
7
4.73 %
0
0.00 %
Humberside
PAR-NEW-IN
1
6
0.04 %
1
16.67 %
4
66.67 %
1
16.67 %
1
16.67 %
0
0.00 %
N Yorks
PAR-NEW-IN
1
97
0.70 %
1
1.03 %
92
94.85 %
4
4.12 %
4
4.12 %
0
0.00 %
England
68
9134
66.03 %
374
4.09 %
8392
91.88 %
458
5.01 %
363
3.97 %
5
0.05 %
Table 2a. Summary by County for May 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - High TB
Carms
PAR-IR-EX
1
19
0.14 %
2
10.53 %
17
89.47 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
22
0.16 %
13
59.09 %
9
40.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
6
0.04 %
0
0.00 %
5
83.33 %
2
33.33 %
1
16.67 %
0
0.00 %
PAR-PERSIS
1
294
2.13 %
20
6.80 %
264
89.80 %
13
4.42 %
6
2.04 %
4
1.36 %
PAR-RAPID
7
8
0.06 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-ANOM
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
5
0.04 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
12
0.09 %
2
16.67 %
9
75.00 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-IR-SEV
3
31
0.22 %
3
9.68 %
27
87.10 %
1
3.23 %
1
3.23 %
0
0.00 %
PAR-PERSIS
3
16
0.12 %
1
6.25 %
15
93.75 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-OTHER
1
6
0.04 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.07 %
2
22.22 %
5
55.56 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RECUR
2
6
0.04 %
0
0.00 %
3
50.00 %
3
50.00 %
3
50.00 %
0
0.00 %
Pembs
PAR-IR-EX
3
25
0.18 %
12
48.00 %
13
52.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
23
178
1.29 %
51
28.65 %
118
66.29 %
9
5.06 %
9
5.06 %
0
0.00 %
PAR-OTHER
1
34
0.25 %
0
0.00 %
34
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
8
0.06 %
1
12.50 %
6
75.00 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-RAPID
5
20
0.14 %
4
20.00 %
15
75.00 %
1
5.00 %
1
5.00 %
0
0.00 %
PAR-RECUR
1
63
0.46 %
1
1.59 %
55
87.30 %
7
11.11 %
7
11.11 %
0
0.00 %
SER-FLEXI
4
285
2.06 %
5
1.75 %
264
92.63 %
29
10.18 %
15
5.26 %
1
0.35 %
Powys
PAR-IR-SEV
1
12
0.09 %
8
66.67 %
4
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
1,234
8.92 %
29
2.35 %
1153
93.44 %
22
1.78 %
17
1.38 %
35
2.84 %
PAR-RAPID
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
38
0.27 %
1
2.63 %
33
86.84 %
4
10.53 %
4
10.53 %
0
0.00 %
S Wales
PAR-IR-SEV
1
12
0.09 %
6
50.00 %
6
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-RECUR
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
5
7
0.05 %
1
14.29 %
5
71.43 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-IR-SEV
8
86
0.62 %
12
13.95 %
72
83.72 %
2
2.33 %
2
2.33 %
0
0.00 %
PAR-NEW-IN
5
43
0.31 %
0
0.00 %
38
88.37 %
5
11.63 %
5
11.63 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
3
308
2.23 %
8
2.60 %
286
92.86 %
19
6.17 %
13
4.22 %
1
0.32 %
S Glamorgan
PAR-RAPID
2
5
0.04 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
1
209
1.51 %
0
0.00 %
201
96.17 %
12
5.74 %
7
3.35 %
1
0.48 %
Gwynedd
PAR-NEW-IN
13
888
6.42 %
9
1.01 %
822
92.57 %
74
8.33 %
54
6.08 %
3
0.34 %
PAR-RAPID
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
7
60
0.43 %
2
3.33 %
47
78.33 %
12
20.00 %
11
18.33 %
0
0.00 %
Wales
129
3966
28.67 %
198
4.99 %
3562
89.81 %
220
5.55 %
161
4.06 %
45
1.13 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
4
86
0.62 %
2
2.33 %
69
80.23 %
16
18.60 %
15
17.44 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
7
648
4.68 %
8
1.23 %
593
91.51 %
54
8.33 %
47
7.25 %
0
0.00 %
Scotland
11
734
5.31 %
10
1.36 %
662
90.19 %
70
9.54 %
62
8.45 %
0
0.00 %
Grand Total
Sum:
208
13834
582
12616
748
586
50
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
12
991
1.09 %
33
3.33 %
911
91.93 %
51
5.15 %
45
4.54 %
2
0.20 %
Corn & Scilly
PAR-CUL-N
3
926
1.02 %
60
6.48 %
804
86.83 %
80
8.64 %
62
6.70 %
0
0.00 %
PAR-CUL-P
1
539
0.59 %
31
5.75 %
481
89.24 %
32
5.94 %
26
4.82 %
1
0.19 %
PAR-HERD-S
1
61
0.07 %
1
1.64 %
59
96.72 %
4
6.56 %
1
1.64 %
0
0.00 %
PAR-OTHER
3
324
0.36 %
24
7.41 %
271
83.64 %
33
10.19 %
29
8.95 %
0
0.00 %
PAR-PERSIS
4
541
0.59 %
42
7.76 %
461
85.21 %
43
7.95 %
38
7.02 %
0
0.00 %
PAR-RECUR
38
8,366
9.18 %
319
3.81 %
7475
89.35 %
651
7.78 %
569
6.80 %
3
0.04 %
Devon
PAR-CUL-N
9
1,406
1.54 %
74
5.26 %
1250
88.90 %
92
6.54 %
76
5.41 %
6
0.43 %
PAR-HERD-S
2
396
0.43 %
35
8.84 %
326
82.32 %
48
12.12 %
35
8.84 %
0
0.00 %
PAR-PERSIS
3
918
1.01 %
38
4.14 %
860
93.68 %
30
3.27 %
20
2.18 %
0
0.00 %
PAR-RECUR
35
5,856
6.42 %
384
6.56 %
4916
83.95 %
366
6.25 %
289
4.94 %
267
4.56 %
Dorset
PAR-RECUR
2
717
0.79 %
12
1.67 %
321
44.77 %
22
3.07 %
22
3.07 %
362
50.49 %
Gloucs
PAR-CUL-N
2
35
0.04 %
5
14.29 %
30
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
541
0.59 %
25
4.62 %
479
88.54 %
43
7.95 %
37
6.84 %
0
0.00 %
Heref
PAR-OTHER
2
83
0.09 %
3
3.61 %
74
89.16 %
6
7.23 %
6
7.23 %
0
0.00 %
PAR-RECUR
18
3,760
4.12 %
229
6.09 %
3297
87.69 %
270
7.18 %
234
6.22 %
0
0.00 %
Shropshire
PAR-RECUR
30
2,960
3.25 %
125
4.22 %
2605
88.01 %
279
9.43 %
228
7.70 %
2
0.07 %
Somerset excl N
PAR-CHR
2
142
0.16 %
22
15.49 %
112
78.87 %
12
8.45 %
8
5.63 %
0
0.00 %
PAR-OTHER
2
12
0.01 %
2
16.67 %
9
75.00 %
2
16.67 %
1
8.33 %
0
0.00 %
PAR-PERSIS
2
296
0.32 %
47
15.88 %
226
76.35 %
25
8.45 %
23
7.77 %
0
0.00 %
PAR-RECUR
18
2,560
2.81 %
155
6.05 %
2319
90.59 %
100
3.91 %
83
3.24 %
3
0.12 %
Staffs
PAR-CHR
6
1,550
1.70 %
57
3.68 %
1362
87.87 %
145
9.35 %
130
8.39 %
1
0.06 %
PAR-OTHER
1
38
0.04 %
1
2.63 %
37
97.37 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
25
5,087
5.58 %
112
2.20 %
4597
90.37 %
397
7.80 %
362
7.12 %
16
0.31 %
Wiltshire
PAR-CUL-N
1
5
0.01 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
10
1,379
1.51 %
54
3.92 %
1270
92.10 %
71
5.15 %
51
3.70 %
4
0.29 %
Table 2b. Summary by County for 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - Edge
Berks
PAR-NEW-IN
4
784
0.86 %
10
1.28 %
726
92.60 %
50
6.38 %
48
6.12 %
0
0.00 %
PAR-RECUR
1
132
0.14 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-CUL-N
1
191
0.21 %
3
1.57 %
182
95.29 %
6
3.14 %
6
3.14 %
0
0.00 %
PAR-NEW-IN
16
1,648
1.81 %
44
2.67 %
1536
93.20 %
73
4.43 %
67
4.07 %
1
0.06 %
PAR-RECUR
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Cheshire
PAR-OTHER
3
516
0.57 %
16
3.10 %
489
94.77 %
11
2.13 %
11
2.13 %
0
0.00 %
PAR-PERSIS
8
1,187
1.30 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
PAR-RECUR
2
215
0.24 %
2
0.93 %
201
93.49 %
15
6.98 %
12
5.58 %
0
0.00 %
Derbyshire
PAR-NEW-IN
10
684
0.75 %
38
5.56 %
603
88.16 %
53
7.75 %
43
6.29 %
0
0.00 %
PAR-RECUR
6
1,306
1.43 %
36
2.76 %
1185
90.74 %
93
7.12 %
85
6.51 %
0
0.00 %
E Sussex
PAR-NEW-IN
13
1,074
1.18 %
21
1.96 %
986
91.81 %
82
7.64 %
67
6.24 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
799
0.88 %
32
4.01 %
724
90.61 %
52
6.51 %
41
5.13 %
2
0.25 %
Leics & Rut
PAR-NEW-IN
15
1,912
2.10 %
56
2.93 %
1772
92.68 %
100
5.23 %
84
4.39 %
0
0.00 %
PAR-RECUR
1
135
0.15 %
11
8.15 %
120
88.89 %
7
5.19 %
4
2.96 %
0
0.00 %
Northants
PAR-NEW-IN
11
1,176
1.29 %
25
2.13 %
951
80.87 %
95
8.08 %
81
6.89 %
119
10.12 %
PAR-RECUR
2
408
0.45 %
6
1.47 %
374
91.67 %
32
7.84 %
28
6.86 %
0
0.00 %
Notts
PAR-NEW-IN
7
301
0.33 %
10
3.32 %
270
89.70 %
37
12.29 %
19
6.31 %
2
0.66 %
Oxon
PAR-NEW-IN
3
250
0.27 %
4
1.60 %
228
91.20 %
23
9.20 %
18
7.20 %
0
0.00 %
PAR-PERSIS
1
235
0.26 %
14
5.96 %
205
87.23 %
16
6.81 %
16
6.81 %
0
0.00 %
PAR-RECUR
9
729
0.80 %
46
6.31 %
639
87.65 %
53
7.27 %
44
6.04 %
0
0.00 %
SER-FLEXI
2
55
0.06 %
3
5.45 %
48
87.27 %
4
7.27 %
4
7.27 %
0
0.00 %
Warks
PAR-NEW-IN
7
683
0.75 %
22
3.22 %
638
93.41 %
34
4.98 %
23
3.37 %
0
0.00 %
PAR-OTHER
1
19
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
19
100.00 %
PAR-RECUR
10
152
0.17 %
5
3.29 %
116
76.32 %
19
12.50 %
17
11.18 %
14
9.21 %
England - LRA
Cumbria
PAR-NEW-IN
11
2,408
2.64 %
68
2.82 %
2149
89.24 %
214
8.89 %
191
7.93 %
0
0.00 %
Essex
PAR-NEW-IN
4
353
0.39 %
1
0.28 %
173
49.01 %
4
1.13 %
2
0.57 %
177
50.14 %
Gr Manchester
PAR-PERSIS
1
41
0.04 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
Humberside
PAR-NEW-IN
2
68
0.07 %
1
1.47 %
60
88.24 %
10
14.71 %
6
8.82 %
1
1.47 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Lancashire
PAR-NEW-IN
1
75
0.08 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Lincs
PAR-NEW-IN
8
950
1.04 %
18
1.89 %
851
89.58 %
85
8.95 %
81
8.53 %
0
0.00 %
N Yorks
PAR-NEW-IN
3
179
0.20 %
2
1.12 %
170
94.97 %
8
4.47 %
7
3.91 %
0
0.00 %
Surrey
PAR-NEW-IN
4
507
0.56 %
22
4.34 %
358
70.61 %
29
5.72 %
29
5.72 %
98
19.33 %
W Yorks
PAR-NEW-IN
1
69
0.08 %
0
0.00 %
65
94.20 %
5
7.25 %
4
5.80 %
0
0.00 %
England
413
58731
64.43 %
2444
4.16 %
51645
87.93 %
4156
7.08 %
3541
6.03 %
1101
1.87 %
Wales - High TB
Carms
PAR-IR-EX
6
36
0.04 %
10
27.78 %
26
72.22 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
20
133
0.15 %
56
42.11 %
70
52.63 %
7
5.26 %
7
5.26 %
0
0.00 %
PAR-OTHER
3
82
0.09 %
1
1.22 %
75
91.46 %
7
8.54 %
6
7.32 %
0
0.00 %
PAR-PERSIS
7
420
0.46 %
20
4.76 %
374
89.05 %
27
6.43 %
18
4.29 %
8
1.90 %
PAR-RAPID
33
83
0.09 %
24
28.92 %
54
65.06 %
5
6.02 %
4
4.82 %
1
1.20 %
PAR-RECUR
7
447
0.49 %
37
8.28 %
395
88.37 %
16
3.58 %
14
3.13 %
1
0.22 %
SER-ANOM
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
11
695
0.76 %
50
7.19 %
610
87.77 %
41
5.90 %
32
4.60 %
3
0.43 %
SER-FRAUD
1
14
0.02 %
0
0.00 %
13
92.86 %
1
7.14 %
1
7.14 %
0
0.00 %
Ceredigion
PAR-IR-EX
6
28
0.03 %
4
14.29 %
21
75.00 %
3
10.71 %
3
10.71 %
0
0.00 %
PAR-IR-SEV
10
52
0.06 %
10
19.23 %
38
73.08 %
4
7.69 %
4
7.69 %
0
0.00 %
PAR-PERSIS
5
630
0.69 %
29
4.60 %
585
92.86 %
20
3.17 %
14
2.22 %
2
0.32 %
PAR-RAPID
8
16
0.02 %
4
25.00 %
11
68.75 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-RECUR
3
147
0.16 %
6
4.08 %
137
93.20 %
3
2.04 %
3
2.04 %
1
0.68 %
SER-FLEXI
9
490
0.54 %
86
17.55 %
380
77.55 %
37
7.55 %
24
4.90 %
0
0.00 %
Gwent
PAR-IR-EX
2
14
0.02 %
9
64.29 %
5
35.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
16
0.02 %
9
56.25 %
6
37.50 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-OTHER
1
6
0.01 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
10
24
0.03 %
5
20.83 %
17
70.83 %
3
12.50 %
2
8.33 %
0
0.00 %
PAR-RECUR
5
388
0.43 %
22
5.67 %
352
90.72 %
21
5.41 %
13
3.35 %
1
0.26 %
SER-FLEXI
9
719
0.79 %
49
6.82 %
632
87.90 %
45
6.26 %
34
4.73 %
4
0.56 %
Pembs
PAR-IR-EX
27
106
0.12 %
46
43.40 %
56
52.83 %
3
2.83 %
3
2.83 %
1
0.94 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
86
586
0.64 %
193
32.94 %
356
60.75 %
43
7.34 %
26
4.44 %
11
1.88 %
PAR-OTHER
2
48
0.05 %
5
10.42 %
42
87.50 %
1
2.08 %
1
2.08 %
0
0.00 %
PAR-PERSIS
21
1,373
1.51 %
113
8.23 %
1150
83.76 %
132
9.61 %
105
7.65 %
5
0.36 %
PAR-RAPID
43
136
0.15 %
34
25.00 %
94
69.12 %
10
7.35 %
7
5.15 %
1
0.74 %
PAR-RECUR
17
1,298
1.42 %
67
5.16 %
1105
85.13 %
106
8.17 %
87
6.70 %
39
3.00 %
SER-FLEXI
18
1,303
1.43 %
73
5.60 %
1162
89.18 %
106
8.14 %
66
5.07 %
2
0.15 %
Powys
PAR-IR-EX
5
8
0.01 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-IR-SEV
15
109
0.12 %
22
20.18 %
77
70.64 %
13
11.93 %
9
8.26 %
1
0.92 %
PAR-NEW-IN
2
152
0.17 %
13
8.55 %
131
86.18 %
10
6.58 %
8
5.26 %
0
0.00 %
PAR-PERSIS
5
1,238
1.36 %
29
2.34 %
1155
93.30 %
23
1.86 %
18
1.45 %
36
2.91 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
17
29
0.03 %
3
10.34 %
26
89.66 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
9
345
0.38 %
15
4.35 %
269
77.97 %
63
18.26 %
58
xx-xxxx-xxxx %
3
0.87 %
S Wales
PAR-IR-SEV
2
21
0.02 %
12
57.14 %
9
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
4
121
0.13 %
6
4.96 %
99
81.82 %
19
15.70 %
15
12.40 %
1
0.83 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
253
0.28 %
43
17.00 %
195
77.08 %
15
5.93 %
14
5.53 %
1
0.40 %
PAR-RAPID
4
9
0.01 %
4
44.44 %
5
55.56 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
7
493
0.54 %
23
4.67 %
442
89.66 %
27
5.48 %
23
4.67 %
5
1.01 %
Ceredigion
PAR-IR-EX
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
341
0.37 %
45
13.20 %
271
79.47 %
29
8.50 %
25
7.33 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
12
0.01 %
0
0.00 %
9
75.00 %
5
41.67 %
3
25.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
NE Wales
PAR-IR-EX
15
37
0.04 %
8
21.62 %
26
70.27 %
3
8.11 %
3
8.11 %
0
0.00 %
PAR-IR-SEV
16
159
0.17 %
26
16.35 %
130
81.76 %
3
1.89 %
3
1.89 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
42
3,123
3.43 %
83
2.66 %
2803
89.75 %
246
7.88 %
217
6.95 %
20
0.64 %
PAR-OTHER
1
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
128
0.14 %
7
5.47 %
106
82.81 %
18
14.06 %
15
11.72 %
0
0.00 %
PAR-POSTM
12
65
0.07 %
6
9.23 %
56
86.15 %
4
6.15 %
3
4.62 %
0
0.00 %
PAR-RAPID
14
29
0.03 %
6
20.69 %
22
75.86 %
1
3.45 %
1
3.45 %
0
0.00 %
PAR-RECUR
1
56
0.06 %
1
1.79 %
50
89.29 %
6
10.71 %
5
8.93 %
0
0.00 %
SER-FLEXI
4
86
0.09 %
6
6.98 %
75
87.21 %
5
5.81 %
5
5.81 %
0
0.00 %
Powys
PAR-IR-EX
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
547
0.60 %
17
3.11 %
501
91.59 %
36
6.58 %
28
5.12 %
1
0.18 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
128
0.14 %
0
0.00 %
118
92.19 %
13
10.16 %
10
7.81 %
0
0.00 %
S Glamorgan
PAR-RAPID
3
6
0.01 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-PERSIS
2
5
0.01 %
0
0.00 %
5
100.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
1
209
0.23 %
0
0.00 %
201
96.17 %
12
5.74 %
7
3.35 %
1
0.48 %
Gwynedd
PAR-IR-SEV
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
74
5,520
6.06 %
142
2.57 %
4995
90.49 %
450
8.15 %
359
6.50 %
24
0.43 %
PAR-OTHER
2
11
0.01 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-PERSIS
1
7
0.01 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
5
5
0.01 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
291
0.32 %
10
3.44 %
267
91.75 %
18
6.19 %
13
4.47 %
1
0.34 %
NE Wales
PAR-IR-EX
2
3
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
5
0.01 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
47
3,569
3.92 %
83
2.33 %
3017
84.53 %
419
11.74 %
233
6.53 %
236
6.61 %
PAR-POSTM
9
23
0.03 %
3
13.04 %
20
86.96 %
1
4.35 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
4
165
0.18 %
12
7.27 %
138
83.64 %
17
10.30 %
15
9.09 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
NW Wales
PAR-NEW-IN
2
24
0.03 %
0
0.00 %
23
95.83 %
1
4.17 %
1
4.17 %
0
0.00 %
Wales
752
26668
29.26 %
1609
6.03 %
23074
86.52 %
2110
7.91 %
1575
5.91 %
410
1.54 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
14
1,338
1.47 %
86
6.43 %
1104
82.51 %
160
11.96 %
143
10.69 %
5
0.37 %
Kirkcudbright
PAR-CUL-N
1
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
15
2,405
2.64 %
125
5.20 %
2070
86.07 %
228
9.48 %
210
8.73 %
0
0.00 %
West lothian
PAR-NEW-IN
9
797
0.87 %
31
3.89 %
640
80.30 %
61
7.65 %
58
7.28 %
68
8.53 %
Wigtown
PAR-NEW-IN
19
1,208
1.33 %
110
9.11 %
994
82.28 %
108
8.94 %
103
8.53 %
1
0.08 %
Scotland
58
5757
6.32 %
352
6.11 %
4816
83.65 %
558
9.69 %
515
8.95 %
74
1.29 %
Grand Total
Sum:
1223
91156
4405
79535
6824
5631
1585
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol May 2023
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
68
9134
100.0%
374
4.1%
8392
91.9%
458
5.0%
363
4.0%
5
0.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
68
9134
100.0%
374
4.1%
8392
91.9%
458
5.0%
363
4.0%
5
0.1%
Scotland
Parallel
11
734
100.0%
10
1.4%
662
90.2%
70
9.5%
62
8.4%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
11
734
100.0%
10
1.4%
662
90.2%
70
9.5%
62
8.4%
0
0.0 %
Wales
Parallel
122
3674
92.6%
193
5.3%
3291
89.6%
191
5.2%
146
4.0%
44
1.2%
Serial/Extended
7
292
7.4%
5
1.7%
271
92.8%
29
9.9%
15
5.1%
1
0.3%
Total
129
3966
100.0%
198
5.0%
3562
89.8%
220
5.5%
161
4.1%
45
1.1%
GB
Parallel
201
13542
97.9%
577
4.3%
12345
91.2%
719
5.3%
571
4.2%
49
0.4%
Serial/Extended
7
292
2.1%
5
1.7%
271
92.8%
29
9.9%
15
5.1%
1
0.3%
Total
208
13834
100.0%
582
4.2%
12616
91.2%
748
5.4%
586
4.2%
50
0.4%
68.00
129.00
11.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2023)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
411
58676
99.9%
2441
4.2%
51597
87.9%
4152
7.1%
3537
6.0%
1101
1.9%
Serial/Extended
2
55
0.1%
3
5.5%
48
87.3%
4
7.3%
4
7.3%
0
0.0 %
Total
413
58731
100.0%
2444
4.2%
51645
87.9%
4156
7.1%
3541
6.0%
1101
1.9%
Scotland
Parallel
58
5757
100.0%
352
6.1%
4816
83.7%
558
9.7%
515
8.9%
74
1.3%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
58
5757
100.0%
352
6.1%
4816
83.7%
558
9.7%
515
8.9%
74
1.3%
Wales
Parallel
694
23194
87.0%
1333
5.7%
20062
86.5%
1858
8.0%
1398
6.0%
401
1.7%
Serial/Extended
58
3474
13.0%
276
7.9%
3012
86.7%
252
7.3%
177
5.1%
9
0.3%
Total
752
26668
100.0%
1609
6.0%
23074
86.5%
2110
7.9%
1575
5.9%
410
1.5%
GB
Parallel
1163
87627
96.1%
4126
4.7%
76475
87.3%
6568
7.5%
5450
6.2%
1576
1.8%
Serial/Extended
60
3529
3.9%
279
7.9%
3060
86.7%
256
7.3%
181
5.1%
9
0.3%
Total
1223
91156
100.0%
4405
4.8%
79535
87.3%
6824
7.5%
5631
6.2%
1585
1.7%
413.00
752.00
58.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
5
290
2.1%
5
1.7%
269
92.8%
29
10.0 %
15
5.2 %
1
0.3 %
Parallel – chronic breakdown
5
667
4.8%
37
5.5%
598
89.7%
40
6.0 %
32
4.8 %
0
Parallel - Herd or Group Slaughter
1
61
0.4%
1
1.6%
59
96.7%
4
6.6 %
1
1.6 %
0
Parallel - IRs in Exit Strategy for persistent her
11
65
0.5%
18
27.7%
45
69.2%
2
3.1 %
2
3.1 %
0
Parallel New Infection
56
3344
24.2%
48
1.4%
3072
91.9%
268
8.0 %
218
6.5 %
6
0.2 %
Parallel - new infection cull area
4
254
1.8%
13
5.1%
224
88.2%
24
9.4 %
16
6.3 %
1
0.4 %
Parallel - Other
5
47
0.3%
0
0.0 %
46
97.9%
2
4.3 %
1
2.1 %
0
Parallel Persistent Infection
11
2470
17.9%
89
3.6%
2298
93.0%
66
2.7 %
44
1.8 %
39
1.6 %
Parallel - Rapid Testing Two-times IRs
24
50
0.4%
9
18.0%
38
76.0%
3
6.0 %
3
6.0 %
0
Parallel - Recurrent breakdowns
45
6243
45.1%
269
4.3%
5729
91.8%
298
4.8 %
242
3.9 %
3
0.0 %
Parallel - severe IRs - in persistent herds
39
341
2.5%
93
27.3%
236
69.2%
12
3.5 %
12
3.5 %
0
Total
208
13834
100.0%
582
4.2%
12616
91.2%
748
5.4 %
586
4.2 %
50
0.4 %
Table 4a. Summaryof IFN Gamma results by submission reason (May 2023)
England - HRA
Parallel – chronic breakdown
5
667
4.8%
37
5.5%
598
89.7%
40
6.0 %
32
4.8 %
0
Parallel - Herd or Group Slaughter
1
61
0.4%
1
1.6%
59
96.7%
4
6.6 %
1
1.6 %
0
Parallel - new infection cull area
4
254
1.8%
13
5.1%
224
88.2%
24
9.4 %
16
6.3 %
1
0.4 %
Parallel Persistent Infection
3
918
6.6%
38
4.1%
860
93.7%
30
3.3 %
20
2.2 %
0
Parallel - Recurrent breakdowns
35
5776
41.8%
248
4.3%
5310
91.9%
267
4.6 %
215
3.7 %
3
0.1 %
England - HRA
48
7676
55.5%
337
4.4%
7051
91.9%
365
4.8 %
284
3.7 %
4
0.1 %
England - Edge
Parallel New Infection
13
851
6.2%
14
1.6%
782
91.9%
64
7.5 %
54
6.3 %
1
0.1 %
Parallel - Recurrent breakdowns
4
356
2.6%
18
5.1%
325
91.3%
17
4.8 %
13
3.7 %
0
England - Edge
17
1207
8.7%
32
2.7%
1107
91.7%
81
6.7 %
67
5.6 %
1
0.1 %
England - LRA
Parallel New Infection
3
251
1.8%
5
2.0%
234
93.2%
12
4.8 %
12
4.8 %
0
England - LRA
3
251
1.8%
5
2.0%
234
93.2%
12
4.8 %
12
4.8 %
0
Wales - High TB
Anomalous reaction
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
5
290
2.1%
5
1.7%
269
92.8%
29
10.0 %
15
5.2 %
1
0.3 %
Parallel - IRs in Exit Strategy for persistent her
5
56
0.4%
16
28.6%
39
69.6%
1
1.8 %
1
1.8 %
0
Parallel - Other
5
47
0.3%
0
0.0 %
46
97.9%
2
4.3 %
1
2.1 %
0
Parallel Persistent Infection
8
1552
11.2%
51
3.3%
1438
92.7%
36
2.3 %
24
1.5 %
39
2.5 %
Parallel - Rapid Testing Two-times IRs
20
42
0.3%
7
16.7%
32
76.2%
3
7.1 %
3
7.1 %
0
Parallel - Recurrent breakdowns
5
108
0.8%
3
2.8%
91
84.3%
14
13.0 %
14
13.0 %
0
Parallel - severe IRs - in persistent herds
31
255
1.8%
81
31.8%
164
64.3%
10
3.9 %
10
3.9 %
0
Wales - High TB
81
2352
17.0%
163
6.9%
2081
88.5%
95
4.0 %
68
2.9 %
40
1.7 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
5
7
0.1%
1
14.3%
5
71.4%
1
14.3 %
1
14.3 %
0
Parallel New Infection
8
351
2.5%
8
2.3%
324
92.3%
24
6.8 %
18
5.1 %
1
0.3 %
Parallel - Rapid Testing Two-times IRs
3
6
0.0%
1
16.7%
5
83.3%
0
0
0
Parallel - Recurrent breakdowns
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Parallel - severe IRs - in persistent herds
8
86
0.6%
12
14.0%
72
83.7%
2
2.3 %
2
2.3 %
0
Wales - Intermediate TB
25
453
3.3%
22
4.9%
409
90.3%
27
6.0 %
21
4.6 %
1
0.2 %
Wales - Low TB
Parallel - IRs in Exit Strategy for persistent her
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel New Infection
21
1157
8.4%
11
1.0%
1070
92.5%
98
8.5 %
72
6.2 %
4
0.3 %
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales - Low TB
23
1161
8.4%
13
1.1%
1072
92.3%
98
8.4 %
72
6.2 %
4
0.3 %
Scotland - Scotland
Parallel New Infection
11
734
5.3%
10
1.4%
662
90.2%
70
9.5 %
62
8.4 %
0
Scotland - Scotland
11
734
5.3%
10
1.4%
662
90.2%
70
9.5 %
62
8.4 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
57
3513
3.9%
279
7.9%
3045
86.7%
255
7.3 %
180
5.1 %
9
0.3 %
Parallel – chronic breakdown
8
xx-xxxx-xxxx
1.9%
79
4.7%
1474
87.1%
157
9.3 %
138
8.2 %
1
0.1 %
Parallel - Herd or Group Slaughter
3
457
0.5%
36
7.9%
385
84.2%
52
11.4 %
36
7.9 %
0
Parallel - IRs in Exit Strategy for persistent herds
66
237
0.3%
81
34.2%
144
60.8%
12
5.1 %
11
4.6 %
1
0.4 %
Parallel New Infection
359
32824
36.0%
1066
3.2%
28820
87.8%
2692
8.2 %
2182
6.6 %
756
2.3 %
Parallel - new infection cull area
17
2572
2.8%
144
5.6%
2277
88.5%
179
7.0 %
145
5.6 %
6
0.2 %
Parallel - Other
29
1523
1.7%
104
6.8%
1312
86.1%
97
6.4 %
86
5.6 %
21
1.4 %
Parallel Persistent Infection
68
7360
8.1%
416
5.7%
6485
88.1%
493
6.7 %
408
5.5 %
51
0.7 %
Parallel - persistent infection cull area
1
539
0.6%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX anti
27
94
0.1%
11
11.7%
80
85.1%
5
5.3 %
3
3.2 %
0
Parallel - Rapid Testing Two-times IRs
142
350
0.4%
84
24.0%
247
70.6%
22
6.3 %
17
4.9 %
2
0.6 %
Parallel - Recurrent breakdowns
283
38888
42.7%
1737
4.5%
34078
87.6%
2756
7.1 %
2348
6.0 %
725
1.9 %
Parallel - severe IRs - in persistent herds
160
1091
1.2%
337
30.9%
692
63.4%
71
6.5 %
50
4.6 %
12
1.1 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Total
1223
91156
100.0%
4405
4.8%
79535
87.3%
6824
7.5 %
5631
6.2 %
1585
1.7 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2023)
England - HRA
Parallel – chronic breakdown
8
xx-xxxx-xxxx
1.9%
79
4.7%
1474
87.1%
157
9.3 %
138
8.2 %
1
0.1 %
Parallel - Herd or Group Slaughter
3
457
0.5%
36
7.9%
385
84.2%
52
11.4 %
36
7.9 %
0
Parallel - new infection cull area
15
2372
2.6%
141
5.9%
2087
88.0%
172
7.3 %
138
5.8 %
6
0.3 %
Parallel - Other
8
457
0.5%
30
6.6%
391
85.6%
41
9.0 %
36
7.9 %
0
Parallel Persistent Infection
9
1755
1.9%
127
7.2%
1547
88.1%
98
5.6 %
81
4.6 %
0
Parallel - persistent infection cull area
1
539
0.6%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel - Recurrent breakdowns
194
32217
35.3%
1448
4.5%
28190
87.5%
2250
7.0 %
1920
6.0 %
659
2.0 %
England - Edge
Extended Flexible IFN-GAMMA test
2
55
0.1%
3
5.5%
48
87.3%
4
7.3 %
4
7.3 %
0
Parallel New Infection
92
9311
10.2%
262
2.8%
8434
90.6%
599
6.4 %
491
5.3 %
124
1.3 %
Parallel - new infection cull area
1
191
0.2%
3
1.6%
182
95.3%
6
3.1 %
6
3.1 %
0
Parallel - Other
4
535
0.6%
16
3.0%
489
91.4%
11
2.1 %
11
2.1 %
19
3.6 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel Persistent Infection
9
1422
1.6%
46
3.2%
1245
87.6%
143
10.1 %
131
9.2 %
0
Parallel - Recurrent breakdowns
32
3078
3.4%
108
3.5%
2753
89.4%
233
7.6 %
202
6.6 %
15
0.5 %
England - LRA
Parallel New Infection
34
4609
5.1%
114
2.5%
3898
84.6%
357
7.7 %
321
7.0 %
276
6.0 %
Parallel Persistent Infection
1
41
0.0%
0
0.0 %
41
100.0%
1
2.4 %
0
0
England
413
58731
64.4%
2444
4.2%
51645
87.9%
4156
7.1 %
3541
6.0 %
1101
1.9 %
Wales - High TB
Anomalous reaction
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
47
3207
3.5%
258
8.0%
2784
86.8%
229
7.1 %
156
4.9 %
9
0.3 %
Parallel - IRs in Exit Strategy for persistent herd
46
192
0.2%
69
35.9%
114
59.4%
8
4.2 %
8
4.2 %
1
0.5 %
Parallel New Infection
2
152
0.2%
13
8.6%
131
86.2%
10
6.6 %
8
5.3 %
0
Parallel - Other
10
257
0.3%
12
4.7%
222
86.4%
27
10.5 %
22
8.6 %
1
0.4 %
Parallel Persistent Infection
38
3661
4.0%
191
5.2%
3264
89.2%
202
5.5 %
155
4.2 %
51
1.4 %
Parallel – post-mvt test coupled with IDEXX ant
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
112
289
0.3%
71
24.6%
202
69.9%
19
6.6 %
14
4.8 %
2
0.7 %
Parallel - Recurrent breakdowns
41
2625
2.9%
147
5.6%
2258
86.0%
209
8.0 %
175
6.7 %
45
1.7 %
Parallel - severe IRs - in persistent herds
135
917
1.0%
302
32.9%
556
60.6%
68
7.4 %
47
5.1 %
12
1.3 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
86
0.1%
6
7.0%
75
87.2%
5
5.8 %
5
5.8 %
0
Parallel - IRs in Exit Strategy for persistent herd
18
42
0.0%
10
23.8%
29
69.0%
3
7.1 %
3
7.1 %
0
Parallel New Infection
50
3682
4.0%
100
2.7%
3313
90.0%
287
7.8 %
248
6.7 %
21
0.6 %
Parallel - Other
5
263
0.3%
45
17.1%
203
77.2%
15
5.7 %
14
5.3 %
1
0.4 %
Parallel Persistent Infection
10
474
0.5%
52
11.0%
382
80.6%
48
10.1 %
40
8.4 %
0
Parallel – post-mvt test coupled with IDEXX ant
12
65
0.1%
6
9.2%
56
86.2%
4
6.2 %
3
4.6 %
0
Parallel - Rapid Testing Two-times IRs
24
49
0.1%
12
24.5%
35
71.4%
2
4.1 %
2
4.1 %
0
Parallel - Recurrent breakdowns
11
677
0.7%
24
3.5%
610
90.1%
46
6.8 %
38
5.6 %
5
0.7 %
Parallel - severe IRs - in persistent herds
21
167
0.2%
31
18.6%
133
79.6%
3
1.8 %
3
1.8 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
165
0.2%
12
7.3%
138
83.6%
17
10.3 %
15
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
2
3
0.0%
2
66.7%
1
33.3%
1
33.3 %
0
0
Parallel New Infection
124
9322
10.2%
225
2.4%
8236
88.4%
882
9.5 %
600
6.4 %
261
2.8 %
Parallel - Other
2
11
0.0%
1
9.1%
7
63.6%
3
27.3 %
3
27.3 %
0
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX ant
14
28
0.0%
5
17.9%
23
82.1%
1
3.6 %
0
0
Parallel - Rapid Testing Two-times IRs
6
12
0.0%
1
8.3%
10
83.3%
1
8.3 %
1
8.3 %
0
Parallel - Recurrent breakdowns
5
291
0.3%
10
3.4%
267
91.8%
18
6.2 %
13
4.5 %
1
0.3 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel - severe IRs - in persistent herds
4
7
0.0%
4
57.1%
3
42.9%
0
0
0
Wales
752
26668
29.3%
1609
6.0%
23074
86.5%
2110
7.9 %
1575
5.9 %
410
1.5 %
Scotland - Scotland
Parallel New Infection
57
5748
6.3%
352
6.1%
4808
83.6%
557
9.7 %
514
8.9 %
74
1.3 %
Parallel - new infection cull area
1
9
0.0%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Scotland
58
5757
6.3%
352
6.1%
4816
83.7%
558
9.7 %
515
8.9 %
74
1.3 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2022 June
213
17755
114
590
19
0
0
0
0
48
1
0
2
0
460
1
57
2
0
July
248
15402
109
485
4
0
0
0
0
46
1
2
3
0
382
1
46
0
0
August
274
19758
144
758
12
0
0
0
0
36
3
0
14
0
642
0
47
1
2
September
205
xx-xxxx-xxxx
107
672
13
0
0
0
0
100
0
1
7
0
520
0
31
0
0
October
180
xx-xxxx-xxxx
100
620
12
0
0
0
0
66
0
0
21
0
495
0
26
0
0
November
201
15848
120
673
16
0
0
0
0
60
1
1
13
0
504
1
77
0
0
December
200
13735
109
545
37
0
0
0
0
26
1
0
10
0
430
0
41
0
0
2023 January
281
23696
176
1138
10
0
0
0
0
83
3
0
17
0
922
1
100
2
0
February
265
17538
147
1036
10
0
0
0
0
69
0
0
31
1
837
0
88
0
0
March
266
19894
159
867
16
0
0
0
0
76
0
1
9
1
696
1
32
0
35
April
203
16194
118
782
1
32
0
0
0
0
53
0
2
1
13
616
24
0
0
36
May
208
13834
112
582
175
200
0
0
0
0
2
0
0
0
0
32
1
0
0
0
Totals (last
12 months)
2744
206494
1515
8748
176
381
0
0
0
0
665
10
7
128
15
6536
30
545
5
73
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
972
135019
653
4899
221
0
0
0
0
522
1
4
21
5
3845
1
12
1
6
Scotland
85
9192
55
456
9
0
0
0
0
117
2
1
28
3
271
3
20
0
0
Wales
xx-xxxx-xxxx
62283
807
3393
151
0
0
0
0
26
7
2
79
7
2420
26
513
4
67
Totals
2744
206494
1515
8748
381
0
0
0
0
665
10
7
128
15
6536
30
545
5
73
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2021
2022
2023
IFNg Positive herds
IFNg Negative herds
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2021
2022
2023
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2021
2022
2023
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
Wiltshire
S Wales
Somerset excl N
S Glamorgan
Pembs
N Yorks
NE Wales
Kirkcudbright
Heref
Gwent
E Sussex
Derbyshire
Corn & Scilly
Ceredigion
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during May 2023 (% submission per County)
County
%Resampled %Retested
Avon
3.19 %
3.94 %
Ayrshire
17.44 %
18.60 %
Bucks
5.49 %
6.75 %
Carms
1.94 %
4.17 %
Ceredigion
3.23 %
3.23 %
Conwy
3.35 %
5.74 %
Corn & Scilly
5.02 %
6.63 %
Cumbria
4.73 %
4.73 %
Derbyshire
1.47 %
1.47 %
Devon
3.34 %
4.64 %
E Sussex
8.75 %
10.00 %
Gloucs
Gwent
23.81 %
23.81 %
Gwynedd
6.07 %
8.31 %
Heref
2.71 %
3.28 %
Humberside
16.67 %
16.67 %
Kirkcudbright
7.25 %
8.33 %
Leics & Rut
25.00 %
25.00 %
NE Wales
9.55 %
10.05 %
Northants
6.94 %
8.16 %
N Yorks
4.12 %
4.12 %
Oxon
3.39 %
4.60 %
Pembs
5.38 %
7.67 %
Powys
2.13 %
2.82 %
S Glamorgan
Shropshire
5.19 %
5.90 %
Somerset excl
2.74 %
3.59 %
Staffs
3.70 %
4.40 %
S Wales
W Glamorgan
Wiltshire
5.31 %
6.12 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
9098
374
4.1%
9134
374
4.1%
Wales
3940
198
5.0%
3966
198
5.0%
Scotland
711
10
1.4%
734
10
1.4%
GB
13749
582
4.2%
13834
582
4.2%
Table 6a (i): Number of animals and submissions tested in May 2023
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
55856
2444
4.4%
58731
2444
4.2%
Wales
25099
1609
6.4%
26668
1609
6.0%
Scotland
4105
352
8.6%
5757
352
6.1%
GB
85042
4405
5.2%
91156
4405
4.8%
Table 6b (i): Number of animals and submissions tested in 2023 Up to end of May
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
18883
668
3.5%
19002
668
3.5%
Wales
4379
240
5.5%
4430
240
5.4%
Scotland
480
10
2.1%
517
10
1.9%
GB
23742
918
3.9%
23949
918
3.8%
Table 6a (ii): Number of animals and submissions tested in May 2022
Table 6b (ii): Number of animals and submissions tested in 2022 Up to end of May
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
72565
3095
4.3%
77598
3095
4.0%
Wales
19177
1414
7.4%
20231
1414
7.0%
Scotland
933
22
2.4%
994
22
2.2%
GB
92674
4531
4.9%
98823
4531
4.6%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
51
2
3.92 %
Kent
PRV-SP
1
7
0
0
West Sussex
PRV-SE
1
28
0
0
Total
4
88
2
2.27 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples May 2023
Table 7b Number of private samples 2023
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
2
3
0
0
Cumbria
PRV-SE
1
3
3
100.00 %
Devon
PRV-SE
5
102
10
9.80 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
51
2
3.92 %
Kent
PRV-SP
1
7
0
0
Oxfordshire
PRV-SP
2
2
0
0
Shropshire
PRV-SP
2
2
0
0
West Sussex
PRV-SE
4
553
24
4.34 %
PRV-SP
1
5
0
0
Total
20
730
39
5.34 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
5
33
11
33.3%
BOV-IR-EX
1
19
3
15.8%
Ceredigion
BOV-IR-C
4
34
9
26.5%
BOV-OTHER
1
33
1
3.0%
North East Wales
BOV-IR-C
4
50
4
8.0%
BOV-P
1
53
2
3.8%
Pembrokeshire
BOV-IR-C
20
137
33
24.1%
BOV-IR-EX
7
47
8
17.0%
BOV-OTHER
1
48
11
22.9%
Powys
BOV-IR-C
4
22
5
22.7%
South Wales
BOV-IR-C
2
16
5
31.3%
BOV-P
1
44
2
4.5%
Wales
51
536
94
17.5%
All GB
51
536
94
17.5 %
Table 8a Number of Cattle Antibody Tests May 2023
Table 8b Number of Cattle Antibody Tests 2023
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
569
38
6.7%
Northern Somerset &
South
Gloucestershire
BOV-P
1
5
0
0
England
3
574
38
6.6%
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
17
148
42
28.4%
BOV-IR-EX
6
36
5
13.9%
BOV-OTHER
1
34
9
26.5%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
14
122
19
15.6%
BOV-IR-EX
2
13
3
23.1%
BOV-OTHER
2
113
3
2.7%
North East Wales
BOV-IR-C
13
121
23
19.0%
BOV-IR-EX
2
11
0
0
BOV-OTHER
1
54
0
0
BOV-P
2
67
2
3.0%
BOV-POSTM
8
21
5
23.8%
Pembrokeshire
BOV-IR-C
71
615
111
18.0%
BOV-IR-EX
17
109
18
16.5%
BOV-OTHER
5
394
18
4.6%
BOV-P
1
28
1
3.6%
Powys
1
8
1
12.5%
BOV-IR-C
20
117
17
14.5%
South Wales
BOV-IR-C
5
42
11
26.2%
BOV-IR-EX
2
14
6
42.9%
BOV-OTHER
1
30
1
3.3%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-P
3
186
6
3.2%
Wales
195
2,332
302
13.0%
All GB
199
2,908
341
11.7 %