Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - July 2023
Source: environment.data.gov.uk (2024-07-03)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2023. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period 1st - 31st July 2023
Issued 11 August 2023
203

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
July 2023
%
Total 2023
%
Submissions
209
1621
Samples
Total (%)
18470
100.00 %
126107
100.00 %
England
13479
72.98 %
81286
64.46 %
Wales
4568
24.73 %
37359
29.62 %
Scotland
423
2.29 %
7462
5.92 %
Parallel Tests
PAR-CHR
93
0.50 %
2334
1.85 %
PAR-CUL-N
476
2.58 %
4340
3.44 %
PAR-CUL-P
0
0.00 %
539
0.43 %
PAR-HERD-S
3
0.02 %
460
0.36 %
PAR-IR-EX
77
0.42 %
345
0.27 %
PAR-IR-SEV
179
0.97 %
1440
1.14 %
PAR-NEW-IN
4216
22.83 %
40454
32.08 %
PAR-OTHER
338
1.83 %
2118
1.68 %
PAR-PERSIS
1094
5.92 %
9915
7.86 %
PAR-POSTM
0
0.00 %
94
0.07 %
PAR-RAPID
62
0.34 %
474
0.38 %
PAR-RECUR
11269
61.01 %
55747
44.21 %
Total(% of all samples)
17807
96.41 %
118260
93.78 %
Serial/Extended Tests
SER-ANOM
14
0.08 %
16
0.01 %
SER-FLEXI
649
3.51 %
7815
6.20 %
SER-FRAUD
0
0.00 %
14
0.01 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
2
0.00 %
Total(% of all samples)
663
3.59 %
7847
6.22 %
Total
18470
100.00 %
126107
100.00 %
Retests
Total (% of all samples)
1223
6.62 %
9167
7.27 %
England
800
4.33 %
5682
4.51 %
Wales
392
2.12 %
2824
2.24 %
Scotland
31
0.17 %
661
0.52 %
Resamples
Total (% of all samples)
890
4.82 %
7360
5.84 %
England
588
3.18 %
4669
3.70 %
Wales
279
1.51 %
2101
1.67 %
Scotland
23
0.12 %
590
0.47 %
Rejects
Total (% of all samples)
42
0.23 %
1732
1.37 %
England
29
0.16 %
1196
0.95 %
Wales
11
0.06 %
460
0.36 %
Scotland
2
0.01 %
76
0.06 %
Table 1. Monthly Totals for July 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-ANOM
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
93
0.5 %
PAR-CUL-N
476
2.6 %
PAR-HERD-S
3
0.0 %
PAR-IR-EX
77
0.4 %
PAR-IR-SEV
179
1.0 %
PAR-NEW-IN
4216
22.8 %
PAR-OTHER
338
1.8 %
PAR-PERSIS
1094
5.9 %
PAR-RAPID
62
0.3 %
PAR-RECUR
11269
61.0 %
SER-ANOM
14
0.1 %
SER-FLEXI
649
3.5 %
Total
18470
100.00 %
Figure 1. Number of samples submitted in each category during July 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
Wigtown
Kirkcudbright
S Wales
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
W Midlands
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Lincs
Herts
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during July 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
14000
Wigtown
West lothian
Renfrew
Perthshire
Kirkcudbright
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NW Wales
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Conwy
Cheshire
Ceredigion
Carms
W Yorks
W Sussex
W Midlands
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-FRAUD
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
6
1,360
7.36 %
51
3.75 %
1269
93.31 %
47
3.46 %
36
2.65 %
4
0.29 %
Corn & Scilly
PAR-RECUR
5
630
3.41 %
14
2.22 %
561
89.05 %
74
11.75 %
55
8.73 %
0
0.00 %
Devon
PAR-CUL-N
3
472
2.56 %
15
3.18 %
440
93.22 %
16
3.39 %
11
2.33 %
6
1.27 %
PAR-RECUR
15
2,127
11.52 %
72
3.39 %
1992
93.65 %
99
4.65 %
61
2.87 %
2
0.09 %
Dorset
PAR-OTHER
1
8
0.04 %
1
12.50 %
6
75.00 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-RECUR
6
1,378
7.46 %
131
9.51 %
1213
88.03 %
49
3.56 %
34
2.47 %
0
0.00 %
Gloucs
PAR-RECUR
4
355
1.92 %
20
5.63 %
330
92.96 %
6
1.69 %
5
1.41 %
0
0.00 %
Heref
PAR-RECUR
3
17
0.09 %
2
11.76 %
12
70.59 %
3
17.65 %
3
17.65 %
0
0.00 %
Shropshire
PAR-RECUR
2
465
2.52 %
1
0.22 %
443
95.27 %
31
6.67 %
21
4.52 %
0
0.00 %
Somerset excl N
PAR-CHR
1
93
0.50 %
5
5.38 %
83
89.25 %
5
5.38 %
5
5.38 %
0
0.00 %
PAR-RECUR
4
828
4.48 %
17
2.05 %
798
96.38 %
16
1.93 %
13
1.57 %
0
0.00 %
Staffs
PAR-RECUR
3
756
4.09 %
27
3.57 %
695
91.93 %
44
5.82 %
29
3.84 %
5
0.66 %
Wiltshire
PAR-OTHER
2
221
1.20 %
5
2.26 %
181
81.90 %
43
19.46 %
34
15.38 %
1
0.45 %
PAR-RECUR
5
1,043
5.65 %
20
1.92 %
974
93.38 %
73
7.00 %
47
4.51 %
2
0.19 %
W Midlands
PAR-RECUR
1
144
0.78 %
0
0.00 %
137
95.14 %
17
11.81 %
7
4.86 %
0
0.00 %
England - Edge
Bucks
PAR-RECUR
1
32
0.17 %
0
0.00 %
32
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-CUL-N
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Derbyshire
PAR-RECUR
2
732
3.96 %
20
2.73 %
683
93.31 %
42
5.74 %
29
3.96 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
20
0.11 %
0
0.00 %
15
75.00 %
6
30.00 %
5
25.00 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
443
2.40 %
18
4.06 %
409
92.33 %
18
4.06 %
15
3.39 %
1
0.23 %
Oxon
PAR-RECUR
3
1,066
5.77 %
19
1.78 %
1001
93.90 %
67
6.29 %
46
4.32 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
60
0.32 %
2
3.33 %
55
91.67 %
3
5.00 %
3
5.00 %
0
0.00 %
Herts
PAR-RECUR
1
79
0.43 %
2
2.53 %
76
96.20 %
3
3.80 %
1
1.27 %
0
0.00 %
Lincs
PAR-NEW-IN
1
148
0.80 %
1
0.68 %
145
97.97 %
2
1.35 %
2
1.35 %
0
0.00 %
N Yorks
PAR-NEW-IN
4
594
3.22 %
2
0.34 %
570
95.96 %
21
3.54 %
16
2.69 %
6
1.01 %
W Sussex
PAR-NEW-IN
1
362
1.96 %
4
1.10 %
253
69.89 %
109
30.11 %
105
29.01 %
0
0.00 %
Table 2a. Summary by County for July 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for July 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Yorks
PAR-NEW-IN
1
42
0.23 %
0
0.00 %
36
85.71 %
5
11.90 %
4
9.52 %
2
4.76 %
England
80
13479
72.98 %
449
3.33 %
12413
92.09 %
800
5.94 %
588
4.36 %
29
0.22 %
Wales - High TB
Carms
PAR-IR-EX
2
3
0.02 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-IR-SEV
5
42
0.23 %
7
16.67 %
30
71.43 %
6
14.29 %
5
11.90 %
0
0.00 %
PAR-PERSIS
3
28
0.15 %
0
0.00 %
24
85.71 %
4
14.29 %
4
14.29 %
0
0.00 %
PAR-RAPID
4
14
0.08 %
4
28.57 %
10
71.43 %
1
7.14 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
11
0.06 %
0
0.00 %
8
72.73 %
4
36.36 %
3
27.27 %
0
0.00 %
SER-ANOM
1
14
0.08 %
0
0.00 %
13
92.86 %
1
7.14 %
1
7.14 %
0
0.00 %
SER-FLEXI
4
475
2.57 %
21
4.42 %
447
94.11 %
12
2.53 %
7
1.47 %
0
0.00 %
Ceredigion
PAR-IR-EX
2
5
0.03 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-IR-SEV
2
16
0.09 %
5
31.25 %
11
68.75 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.05 %
2
22.22 %
6
66.67 %
0
0.00 %
0
0.00 %
1
11.11 %
Gwent
PAR-IR-EX
2
14
0.08 %
8
57.14 %
6
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.05 %
1
11.11 %
6
66.67 %
3
33.33 %
2
22.22 %
0
0.00 %
PAR-RECUR
2
83
0.45 %
3
3.61 %
75
90.36 %
9
10.84 %
5
6.02 %
0
0.00 %
SER-FLEXI
3
37
0.20 %
1
2.70 %
33
89.19 %
8
21.62 %
3
8.11 %
0
0.00 %
Pembs
PAR-HERD-S
1
2
0.01 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-IR-EX
6
42
0.23 %
10
23.81 %
27
64.29 %
6
14.29 %
5
11.90 %
0
0.00 %
PAR-IR-SEV
12
108
0.58 %
21
19.44 %
77
71.30 %
14
12.96 %
10
9.26 %
0
0.00 %
PAR-OTHER
4
108
0.58 %
2
1.85 %
88
81.48 %
26
24.07 %
18
16.67 %
0
0.00 %
PAR-PERSIS
8
1,034
5.60 %
9
0.87 %
979
94.68 %
70
6.77 %
42
4.06 %
4
0.39 %
PAR-RAPID
6
20
0.11 %
4
20.00 %
16
80.00 %
1
5.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
62
0.34 %
2
3.23 %
57
91.94 %
2
3.23 %
2
3.23 %
1
1.61 %
Powys
PAR-IR-SEV
2
13
0.07 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
4
0.02 %
0
0.00 %
3
75.00 %
2
50.00 %
1
25.00 %
0
0.00 %
PAR-RAPID
3
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
116
0.63 %
4
3.45 %
103
88.79 %
14
12.07 %
9
7.76 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for July 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
2
75
0.41 %
2
2.67 %
62
82.67 %
11
14.67 %
11
14.67 %
0
0.00 %
S Wales
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
3
13
0.07 %
2
15.38 %
10
76.92 %
1
7.69 %
1
7.69 %
0
0.00 %
PAR-NEW-IN
11
1,115
6.04 %
18
1.61 %
1044
93.63 %
70
6.28 %
50
4.48 %
3
0.27 %
PAR-PERSIS
2
28
0.15 %
0
0.00 %
28
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
47
0.25 %
1
2.13 %
34
72.34 %
19
40.43 %
12
25.53 %
0
0.00 %
Powys
PAR-NEW-IN
1
266
1.44 %
4
1.50 %
225
84.59 %
40
15.04 %
35
13.16 %
2
0.75 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
9
678
3.67 %
8
1.18 %
631
93.07 %
53
7.82 %
39
5.75 %
0
0.00 %
NE Wales
PAR-NEW-IN
1
65
0.35 %
1
1.54 %
52
80.00 %
12
18.46 %
12
18.46 %
0
0.00 %
Wales
125
4568
24.73 %
143
3.13 %
4135
90.52 %
392
8.58 %
279
6.11 %
11
0.24 %
Scotland - Scotland
Kirkcudbright
PAR-NEW-IN
3
398
2.15 %
5
1.26 %
376
94.47 %
21
5.28 %
17
4.27 %
0
0.00 %
Wigtown
PAR-NEW-IN
1
25
0.14 %
0
0.00 %
17
68.00 %
10
40.00 %
6
24.00 %
2
8.00 %
Scotland
4
423
2.29 %
5
1.18 %
393
92.91 %
31
7.33 %
23
5.44 %
2
0.47 %
Grand Total
Sum:
209
18470
597
xx-xxxx-xxxx
1223
890
42
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
PAR-RECUR
1
51
0.04 %
1
1.96 %
49
96.08 %
2
3.92 %
1
1.96 %
0
0.00 %
Avon
PAR-RECUR
19
2,353
1.87 %
84
3.57 %
2182
92.73 %
98
4.16 %
81
3.44 %
6
0.25 %
Corn & Scilly
PAR-CUL-N
3
926
0.73 %
60
6.48 %
804
86.83 %
80
8.64 %
62
6.70 %
0
0.00 %
PAR-CUL-P
1
539
0.43 %
31
5.75 %
481
89.24 %
32
5.94 %
26
4.82 %
1
0.19 %
PAR-HERD-S
1
61
0.05 %
1
1.64 %
59
96.72 %
4
6.56 %
1
1.64 %
0
0.00 %
PAR-OTHER
3
324
0.26 %
24
7.41 %
271
83.64 %
33
10.19 %
29
8.95 %
0
0.00 %
PAR-PERSIS
6
554
0.44 %
42
7.58 %
473
85.38 %
45
8.12 %
39
7.04 %
0
0.00 %
PAR-RECUR
49
9,451
7.49 %
377
3.99 %
8405
88.93 %
777
8.22 %
666
7.05 %
3
0.03 %
Devon
PAR-CHR
2
408
0.32 %
11
2.70 %
373
91.42 %
39
9.56 %
24
5.88 %
0
0.00 %
PAR-CUL-N
13
2,183
1.73 %
96
4.40 %
1976
90.52 %
121
5.54 %
98
4.49 %
13
0.60 %
PAR-HERD-S
2
396
0.31 %
35
8.84 %
326
82.32 %
48
12.12 %
35
8.84 %
0
0.00 %
PAR-PERSIS
4
937
0.74 %
38
4.06 %
873
93.17 %
36
3.84 %
26
2.77 %
0
0.00 %
PAR-RECUR
58
9,631
7.64 %
489
5.08 %
8367
86.88 %
650
6.75 %
506
5.25 %
269
2.79 %
Dorset
PAR-OTHER
2
262
0.21 %
22
8.40 %
230
87.79 %
7
2.67 %
4
1.53 %
6
2.29 %
PAR-RECUR
8
2,095
1.66 %
143
6.83 %
1534
73.22 %
71
3.39 %
56
2.67 %
362
17.28 %
Gloucs
PAR-CUL-N
2
35
0.03 %
5
14.29 %
30
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
10
896
0.71 %
45
5.02 %
809
90.29 %
49
5.47 %
42
4.69 %
0
0.00 %
Heref
PAR-OTHER
2
83
0.07 %
3
3.61 %
74
89.16 %
6
7.23 %
6
7.23 %
0
0.00 %
PAR-RECUR
31
5,247
4.16 %
290
5.53 %
4631
88.26 %
398
7.59 %
326
6.21 %
0
0.00 %
Shropshire
PAR-RECUR
37
4,060
3.22 %
153
3.77 %
3641
89.68 %
326
8.03 %
263
6.48 %
3
0.07 %
Somerset excl N
PAR-CHR
5
364
0.29 %
40
10.99 %
300
82.42 %
34
9.34 %
24
6.59 %
0
0.00 %
PAR-OTHER
2
12
0.01 %
2
16.67 %
9
75.00 %
2
16.67 %
1
8.33 %
0
0.00 %
PAR-PERSIS
2
296
0.23 %
47
15.88 %
226
76.35 %
25
8.45 %
23
7.77 %
0
0.00 %
PAR-RECUR
28
3,801
3.01 %
210
5.52 %
3476
91.45 %
136
3.58 %
111
2.92 %
4
0.11 %
Staffs
PAR-CHR
7
1,562
1.24 %
57
3.65 %
1370
87.71 %
149
9.54 %
134
8.58 %
1
0.06 %
PAR-OTHER
1
38
0.03 %
1
2.63 %
37
97.37 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2b. Summary by County for 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
30
6,359
5.04 %
141
2.22 %
5739
90.25 %
543
8.54 %
454
7.14 %
25
0.39 %
Wiltshire
PAR-CUL-N
3
992
0.79 %
34
3.43 %
924
93.15 %
42
4.23 %
31
3.13 %
3
0.30 %
PAR-OTHER
2
221
0.18 %
5
2.26 %
181
81.90 %
43
19.46 %
34
15.38 %
1
0.45 %
PAR-RECUR
16
2,491
1.98 %
76
3.05 %
2301
92.37 %
154
6.18 %
108
4.34 %
6
0.24 %
W Midlands
PAR-RECUR
1
144
0.11 %
0
0.00 %
137
95.14 %
17
11.81 %
7
4.86 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
4
784
0.62 %
10
1.28 %
726
92.60 %
50
6.38 %
48
6.12 %
0
0.00 %
PAR-RECUR
1
132
0.10 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-CUL-N
1
191
0.15 %
3
1.57 %
182
95.29 %
6
3.14 %
6
3.14 %
0
0.00 %
PAR-NEW-IN
18
1,791
1.42 %
47
2.62 %
1623
90.62 %
77
4.30 %
70
3.91 %
51
2.85 %
PAR-RECUR
2
33
0.03 %
0
0.00 %
32
96.97 %
1
3.03 %
1
3.03 %
0
0.00 %
Cheshire
PAR-CUL-N
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
516
0.41 %
16
3.10 %
489
94.77 %
11
2.13 %
11
2.13 %
0
0.00 %
PAR-PERSIS
8
1,187
0.94 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
PAR-RECUR
3
417
0.33 %
4
0.96 %
397
95.20 %
21
5.04 %
16
3.84 %
0
0.00 %
Derbyshire
PAR-NEW-IN
10
684
0.54 %
38
5.56 %
603
88.16 %
53
7.75 %
43
6.29 %
0
0.00 %
PAR-RECUR
8
2,038
1.62 %
56
2.75 %
1868
91.66 %
135
6.62 %
114
5.59 %
0
0.00 %
E Sussex
PAR-NEW-IN
14
1,094
0.87 %
21
1.92 %
1001
91.50 %
88
8.04 %
72
6.58 %
0
0.00 %
Hampshire
PAR-NEW-IN
8
1,242
0.98 %
50
4.03 %
1133
91.22 %
70
5.64 %
56
4.51 %
3
0.24 %
Leics & Rut
PAR-NEW-IN
17
2,119
1.68 %
58
2.74 %
1975
93.20 %
105
4.96 %
86
4.06 %
0
0.00 %
PAR-RECUR
1
135
0.11 %
11
8.15 %
120
88.89 %
7
5.19 %
4
2.96 %
0
0.00 %
Northants
PAR-NEW-IN
11
1,176
0.93 %
25
2.13 %
951
80.87 %
95
8.08 %
81
6.89 %
119
10.12 %
PAR-RECUR
2
408
0.32 %
6
1.47 %
374
91.67 %
32
7.84 %
28
6.86 %
0
0.00 %
Notts
PAR-NEW-IN
9
553
0.44 %
13
2.35 %
501
90.60 %
60
10.85 %
37
6.69 %
2
0.36 %
Oxon
PAR-NEW-IN
3
250
0.20 %
4
1.60 %
228
91.20 %
23
9.20 %
18
7.20 %
0
0.00 %
PAR-PERSIS
3
976
0.77 %
39
4.00 %
895
91.70 %
57
5.84 %
42
4.30 %
0
0.00 %
PAR-RECUR
14
1,870
1.48 %
65
3.48 %
1713
91.60 %
122
6.52 %
92
4.92 %
0
0.00 %
SER-FLEXI
2
55
0.04 %
3
5.45 %
48
87.27 %
4
7.27 %
4
7.27 %
0
0.00 %
Warks
PAR-NEW-IN
7
683
0.54 %
22
3.22 %
638
93.41 %
34
4.98 %
23
3.37 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
19
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
19
100.00 %
PAR-RECUR
10
152
0.12 %
5
3.29 %
116
76.32 %
19
12.50 %
17
11.18 %
14
9.21 %
England - LRA
Cumbria
PAR-NEW-IN
13
2,475
1.96 %
70
2.83 %
2210
89.29 %
218
8.81 %
195
7.88 %
0
0.00 %
Essex
PAR-NEW-IN
6
405
0.32 %
7
1.73 %
216
53.33 %
7
1.73 %
5
1.23 %
177
43.70 %
Gr Manchester
PAR-PERSIS
1
41
0.03 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
Herts
PAR-RECUR
1
79
0.06 %
2
2.53 %
76
96.20 %
3
3.80 %
1
1.27 %
0
0.00 %
Humberside
PAR-NEW-IN
2
68
0.05 %
1
1.47 %
60
88.24 %
10
14.71 %
6
8.82 %
1
1.47 %
Lancashire
PAR-NEW-IN
1
75
0.06 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Lincs
PAR-NEW-IN
10
1,109
0.88 %
19
1.71 %
1007
90.80 %
87
7.84 %
83
7.48 %
0
0.00 %
N Yorks
PAR-NEW-IN
7
773
0.61 %
4
0.52 %
740
95.73 %
29
3.75 %
23
2.98 %
6
0.78 %
Surrey
PAR-NEW-IN
4
507
0.40 %
22
4.34 %
358
70.61 %
29
5.72 %
29
5.72 %
98
19.33 %
W Sussex
PAR-NEW-IN
1
362
0.29 %
4
1.10 %
253
69.89 %
109
30.11 %
105
29.01 %
0
0.00 %
W Yorks
PAR-NEW-IN
2
111
0.09 %
0
0.00 %
101
90.99 %
10
9.01 %
8
7.21 %
2
1.80 %
England
560
81286
64.46 %
3224
3.97 %
72197
88.82 %
5682
6.99 %
4669
5.74 %
1196
1.47 %
Wales - High TB
Carms
PAR-IR-EX
10
45
0.04 %
10
22.22 %
33
73.33 %
2
4.44 %
2
4.44 %
0
0.00 %
PAR-IR-SEV
27
187
0.15 %
69
36.90 %
106
56.68 %
13
6.95 %
12
6.42 %
0
0.00 %
PAR-OTHER
3
82
0.07 %
1
1.22 %
75
91.46 %
7
8.54 %
6
7.32 %
0
0.00 %
PAR-PERSIS
15
779
0.62 %
32
4.11 %
708
90.89 %
43
5.52 %
31
3.98 %
8
1.03 %
PAR-RAPID
42
112
0.09 %
36
32.14 %
71
63.39 %
6
5.36 %
4
3.57 %
1
0.89 %
PAR-RECUR
11
502
0.40 %
40
7.97 %
434
86.45 %
30
5.98 %
25
4.98 %
3
0.60 %
SER-ANOM
3
16
0.01 %
0
0.00 %
15
93.75 %
1
6.25 %
1
6.25 %
0
0.00 %
SER-FLEXI
15
1,170
0.93 %
71
6.07 %
1057
90.34 %
53
4.53 %
39
3.33 %
3
0.26 %
SER-FRAUD
1
14
0.01 %
0
0.00 %
13
92.86 %
1
7.14 %
1
7.14 %
0
0.00 %
Ceredigion
PAR-IR-EX
10
38
0.03 %
6
15.79 %
27
71.05 %
5
13.16 %
5
13.16 %
0
0.00 %
PAR-IR-SEV
18
114
0.09 %
20
17.54 %
89
78.07 %
6
5.26 %
5
4.39 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
5
630
0.50 %
29
4.60 %
585
92.86 %
20
3.17 %
14
2.22 %
2
0.32 %
PAR-RAPID
16
33
0.03 %
8
24.24 %
22
66.67 %
2
6.06 %
2
6.06 %
1
3.03 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
3
147
0.12 %
6
4.08 %
137
93.20 %
3
2.04 %
3
2.04 %
1
0.68 %
SER-FLEXI
9
490
0.39 %
86
17.55 %
380
77.55 %
37
7.55 %
24
4.90 %
0
0.00 %
Gwent
PAR-IR-EX
4
28
0.02 %
17
60.71 %
11
39.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
19
0.02 %
10
52.63 %
7
36.84 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-OTHER
1
6
0.00 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
16
37
0.03 %
6
16.22 %
27
72.97 %
6
16.22 %
4
10.81 %
0
0.00 %
PAR-RECUR
7
471
0.37 %
25
5.31 %
427
90.66 %
30
6.37 %
18
3.82 %
1
0.21 %
SER-FLEXI
15
1,504
1.19 %
64
4.26 %
1375
91.42 %
77
5.12 %
56
3.72 %
9
0.60 %
Pembs
PAR-HERD-S
1
2
0.00 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-IR-EX
33
148
0.12 %
56
37.84 %
83
56.08 %
9
6.08 %
8
5.41 %
1
0.68 %
PAR-IR-SEV
107
780
0.62 %
226
28.97 %
504
64.62 %
61
7.82 %
39
5.00 %
11
1.41 %
PAR-OTHER
6
156
0.12 %
7
4.49 %
130
83.33 %
27
17.31 %
19
12.18 %
0
0.00 %
PAR-PERSIS
31
2,627
2.08 %
128
4.87 %
2337
88.96 %
208
7.92 %
152
5.79 %
10
0.38 %
PAR-RAPID
60
184
0.15 %
39
21.20 %
137
74.46 %
11
5.98 %
7
3.80 %
1
0.54 %
PAR-RECUR
18
1,304
1.03 %
67
5.14 %
1110
85.12 %
107
8.21 %
88
6.75 %
39
2.99 %
SER-FLEXI
27
1,926
1.53 %
87
4.52 %
1754
91.07 %
127
6.59 %
82
4.26 %
3
0.16 %
Powys
PAR-IR-EX
6
16
0.01 %
1
6.25 %
13
81.25 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-IR-SEV
19
129
0.10 %
22
17.05 %
97
75.19 %
15
11.63 %
9
6.98 %
1
0.78 %
PAR-NEW-IN
2
152
0.12 %
13
8.55 %
131
86.18 %
10
6.58 %
8
5.26 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
9
1,379
1.09 %
31
2.25 %
1284
93.11 %
36
2.61 %
28
2.03 %
36
2.61 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
23
38
0.03 %
4
10.53 %
34
89.47 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
11
465
0.37 %
20
4.30 %
375
80.65 %
77
16.56 %
67
14.41 %
3
0.65 %
SER-FLEXI
7
2,419
1.92 %
54
2.23 %
2248
92.93 %
104
4.30 %
89
3.68 %
28
1.16 %
S Wales
PAR-IR-SEV
2
21
0.02 %
12
57.14 %
9
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
4
121
0.10 %
6
4.96 %
99
81.82 %
19
15.70 %
15
12.40 %
1
0.83 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
253
0.20 %
43
17.00 %
195
77.08 %
15
5.93 %
14
5.53 %
1
0.40 %
PAR-RAPID
5
10
0.01 %
4
40.00 %
6
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
7
493
0.39 %
23
4.67 %
442
89.66 %
27
5.48 %
23
4.67 %
5
1.01 %
Ceredigion
PAR-IR-EX
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
341
0.27 %
45
13.20 %
271
79.47 %
29
8.50 %
25
7.33 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
12
0.01 %
0
0.00 %
9
75.00 %
5
41.67 %
3
25.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
3
5
0.00 %
2
40.00 %
2
40.00 %
1
20.00 %
1
20.00 %
0
0.00 %
NE Wales
PAR-IR-EX
20
57
0.05 %
10
17.54 %
41
71.93 %
7
12.28 %
6
10.53 %
0
0.00 %
PAR-IR-SEV
18
169
0.13 %
29
17.16 %
137
81.07 %
3
1.78 %
3
1.78 %
0
0.00 %
PAR-NEW-IN
58
4,797
3.80 %
109
2.27 %
4359
90.87 %
359
7.48 %
305
6.36 %
24
0.50 %
PAR-OTHER
1
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
8
156
0.12 %
7
4.49 %
134
85.90 %
18
11.54 %
15
9.62 %
0
0.00 %
PAR-POSTM
12
65
0.05 %
6
9.23 %
56
86.15 %
4
6.15 %
3
4.62 %
0
0.00 %
PAR-RAPID
17
33
0.03 %
6
18.18 %
26
78.79 %
1
3.03 %
1
3.03 %
0
0.00 %
PAR-RECUR
2
103
0.08 %
2
1.94 %
84
81.55 %
25
24.27 %
17
16.50 %
0
0.00 %
SER-FLEXI
4
86
0.07 %
6
6.98 %
75
87.21 %
5
5.81 %
5
5.81 %
0
0.00 %
Powys
PAR-IR-EX
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
9
0.01 %
2
22.22 %
6
66.67 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
9
826
0.65 %
21
2.54 %
736
89.10 %
80
9.69 %
66
7.99 %
3
0.36 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
128
0.10 %
0
0.00 %
118
92.19 %
13
10.16 %
10
7.81 %
0
0.00 %
S Glamorgan
PAR-RAPID
3
6
0.00 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-PERSIS
2
5
0.00 %
0
0.00 %
5
100.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
3
217
0.17 %
0
0.00 %
208
95.85 %
13
5.99 %
8
3.69 %
1
0.46 %
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
94
6,591
5.23 %
158
2.40 %
5975
90.65 %
558
8.47 %
434
6.58 %
24
0.36 %
PAR-OTHER
2
11
0.01 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-PERSIS
1
7
0.01 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
5
5
0.00 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
291
0.23 %
10
3.44 %
267
91.75 %
18
6.19 %
13
4.47 %
1
0.34 %
NE Wales
PAR-IR-EX
3
8
0.01 %
2
25.00 %
6
75.00 %
1
12.50 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
5
0.00 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
57
4,125
3.27 %
87
2.11 %
3540
85.82 %
458
11.10 %
260
6.30 %
238
5.77 %
PAR-POSTM
9
23
0.02 %
3
13.04 %
20
86.96 %
1
4.35 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
4
165
0.13 %
12
7.27 %
138
83.64 %
17
10.30 %
15
9.09 %
0
0.00 %
NW Wales
PAR-NEW-IN
2
24
0.02 %
0
0.00 %
23
95.83 %
1
4.17 %
1
4.17 %
0
0.00 %
Wales
992
37359
29.62 %
1915
5.13 %
32883
88.02 %
2824
7.56 %
2101
5.62 %
460
1.23 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
14
1,338
1.06 %
86
6.43 %
1104
82.51 %
160
11.96 %
143
10.69 %
5
0.37 %
Kirkcudbright
PAR-CUL-N
1
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
21
3,919
3.11 %
160
4.08 %
3505
89.44 %
289
7.37 %
254
6.48 %
0
0.00 %
Perthshire
PAR-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Renfrew
SER-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
West lothian
PAR-NEW-IN
9
797
0.63 %
31
3.89 %
640
80.30 %
61
7.65 %
58
7.28 %
68
8.53 %
Wigtown
PAR-NEW-IN
22
1,395
1.11 %
110
7.89 %
1148
82.29 %
149
10.68 %
134
9.61 %
3
0.22 %
Scotland
69
7462
5.92 %
387
5.19 %
6409
85.89 %
661
8.86 %
590
7.91 %
76
1.02 %
Grand Total
Sum:
xx-xxxx-xxxx
5526
111489
9167
7360
1732
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol July 2023
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
80
13479
100.0%
449
3.3%
12413
92.1%
800
5.9%
588
4.4%
29
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
80
13479
100.0%
449
3.3%
12413
92.1%
800
5.9%
588
4.4%
29
0.2%
Scotland
Parallel
4
423
100.0%
5
1.2%
393
92.9%
31
7.3%
23
5.4%
2
0.5%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
4
423
100.0%
5
1.2%
393
92.9%
31
7.3%
23
5.4%
2
0.5%
Wales
Parallel
111
3905
85.5%
117
3.0%
3523
90.2%
358
9.2%
255
6.5%
10
0.3%
Serial/Extended
14
663
14.5%
26
3.9%
612
92.3%
34
5.1%
24
3.6%
1
0.2%
Total
125
4568
100.0%
143
3.1%
4135
90.5%
392
8.6%
279
6.1%
11
0.2%
GB
Parallel
195
17807
96.4%
571
3.2%
16329
91.7%
1189
6.7%
866
4.9%
41
0.2%
Serial/Extended
14
663
3.6%
26
3.9%
612
92.3%
34
5.1%
24
3.6%
1
0.2%
Total
209
18470
100.0%
597
3.2%
xx-xxxx-xxxx
91.7%
1223
6.6%
890
4.8%
42
0.2%
80.00
125.00
4.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2023)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
558
81231
99.9%
3221
4.0%
72149
88.8%
5678
7.0%
4665
5.7%
1196
1.5%
Serial/Extended
2
55
0.1%
3
5.5%
48
87.3%
4
7.3%
4
7.3%
0
0.0 %
Total
560
81286
100.0%
3224
4.0%
72197
88.8%
5682
7.0%
4669
5.7%
1196
1.5%
Scotland
Parallel
68
7460
100.0%
387
5.2%
6407
85.9%
660
8.8%
590
7.9%
76
1.0%
Serial/Extended
1
2
0.0%
0
0.0 %
2 100.0%
1
50.0%
0
0.0 %
0
0.0 %
Total
69
7462
100.0%
387
5.2%
6409
85.9%
661
8.9%
590
7.9%
76
1.0%
Wales
Parallel
907
29569
79.1%
1535
5.2%
25828
87.3%
2402
8.1%
1789
6.1%
417
1.4%
Serial/Extended
85
7790
20.9%
380
4.9%
7055
90.6%
422
5.4%
312
4.0%
43
0.6%
Total
992
37359
100.0%
1915
5.1%
32883
88.0%
2824
7.6%
2101
5.6%
460
1.2%
GB
Parallel
xx-xxxx-xxxx
93.8%
5143
4.3% 104384
88.3%
8740
7.4%
7044
6.0%
xx-xxxx-xxxx
1.4%
Serial/Extended
88
7847
6.2%
383
4.9%
7105
90.5%
427
5.4%
316
4.0%
43
0.5%
Total
xx-xxxx-xxxx
100.0%
5526
4.4% 111489
88.4%
9167
7.3%
7360
5.8%
1732
1.4%
560.00
992.00
69.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
14
0.1%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Extended Flexible IFN-GAMMA test
13
649
3.5%
26
4.0%
599
92.3%
33
5.1 %
23
3.5 %
1
0.2 %
Parallel – chronic breakdown
1
93
0.5%
5
5.4%
83
89.2%
5
5.4 %
5
5.4 %
0
Parallel - Herd or Group Slaughter
2
3
0.0%
0
0.0 %
3
100.0%
1
33.3 %
0
0
Parallel - IRs in Exit Strategy for persistent her
15
77
0.4%
21
27.3%
48
62.3%
9
11.7 %
8
10.4 %
0
Parallel New Infection
37
4216
22.8%
63
1.5%
3828
90.8%
370
8.8 %
309
7.3 %
16
0.4 %
Parallel - new infection cull area
4
476
2.6%
15
3.2%
444
93.3%
16
3.4 %
11
2.3 %
6
1.3 %
Parallel - Other
8
338
1.8%
9
2.7%
275
81.4%
70
20.7 %
53
15.7 %
1
0.3 %
Parallel Persistent Infection
14
1094
5.9%
9
0.8%
1034
94.5%
76
6.9 %
47
4.3 %
4
0.4 %
Parallel - Rapid Testing Two-times IRs
25
62
0.3%
12
19.4%
47
75.8%
5
8.1 %
2
3.2 %
1
1.6 %
Parallel - Recurrent breakdowns
68
11269
61.0%
404
3.6%
10436
92.6%
617
5.5 %
416
3.7 %
13
0.1 %
Parallel - severe IRs - in persistent herds
21
179
1.0%
33
18.4%
131
73.2%
20
11.2 %
15
8.4 %
0
Total
209
18470
100.0%
597
3.2%
xx-xxxx-xxxx
91.7%
1223
6.6 %
890
4.8 %
42
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (July 2023)
England - HRA
Parallel – chronic breakdown
1
93
0.5%
5
5.4%
83
89.2%
5
5.4 %
5
5.4 %
0
Parallel - new infection cull area
3
472
2.6%
15
3.2%
440
93.2%
16
3.4 %
11
2.3 %
6
1.3 %
Parallel - Other
3
229
1.2%
6
2.6%
187
81.7%
44
19.2 %
35
15.3 %
1
0.4 %
Parallel - Recurrent breakdowns
54
9103
49.3%
355
3.9%
8424
92.5%
459
5.0 %
311
3.4 %
13
0.1 %
England - HRA
61
9897
53.6%
381
3.8%
9134
92.3%
524
5.3 %
362
3.7 %
20
0.2 %
England - Edge
Parallel New Infection
3
463
2.5%
18
3.9%
424
91.6%
24
5.2 %
20
4.3 %
1
0.2 %
Parallel - new infection cull area
1
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Parallel - Recurrent breakdowns
6
1830
9.9%
39
2.1%
1716
93.8%
109
6.0 %
75
4.1 %
0
England - Edge
10
2297
12.4%
57
2.5%
2144
93.3%
133
5.8 %
95
4.1 %
1
0.0 %
England - LRA
Parallel New Infection
8
1206
6.5%
9
0.7%
1059
87.8%
140
11.6 %
130
10.8 %
8
0.7 %
Parallel - Recurrent breakdowns
1
79
0.4%
2
2.5%
76
96.2%
3
3.8 %
1
1.3 %
0
England - LRA
9
1285
7.0%
11
0.9%
1135
88.3%
143
11.1 %
131
10.2 %
8
0.6 %
Wales - High TB
Anomalous reaction
1
14
0.1%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Extended Flexible IFN-GAMMA test
13
649
3.5%
26
4.0%
599
92.3%
33
5.1 %
23
3.5 %
1
0.2 %
Parallel - Herd or Group Slaughter
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Parallel - IRs in Exit Strategy for persistent her
12
64
0.3%
19
29.7%
38
59.4%
8
12.5 %
7
10.9 %
0
Parallel - Other
5
109
0.6%
3
2.8%
88
80.7%
26
23.9 %
18
16.5 %
0
Parallel Persistent Infection
12
1066
5.8%
9
0.8%
1006
94.4%
76
7.1 %
47
4.4 %
4
0.4 %
Parallel - Rapid Testing Two-times IRs
22
58
0.3%
11
19.0%
44
75.9%
5
8.6 %
2
3.4 %
1
1.7 %
Parallel - Recurrent breakdowns
6
210
1.1%
7
3.3%
186
88.6%
27
12.9 %
17
8.1 %
0
Parallel - severe IRs - in persistent herds
21
179
1.0%
33
18.4%
131
73.2%
20
11.2 %
15
8.4 %
0
Wales - High TB
93
2351
12.7%
108
4.6%
2107
89.6%
197
8.4 %
130
5.5 %
6
0.3 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
3
13
0.1%
2
15.4%
10
76.9%
1
7.7 %
1
7.7 %
0
Parallel New Infection
12
1381
7.5%
22
1.6%
1269
91.9%
110
8.0 %
85
6.2 %
5
0.4 %
Parallel Persistent Infection
2
28
0.2%
0
0.0 %
28
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
3
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel - Recurrent breakdowns
1
47
0.3%
1
2.1%
34
72.3%
19
40.4 %
12
25.5 %
0
Wales - Intermediate TB
21
1473
8.0%
26
1.8%
1344
91.2%
130
8.8 %
98
6.7 %
5
0.3 %
Wales - Low TB
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
10
743
4.0%
9
1.2%
683
91.9%
65
8.7 %
51
6.9 %
0
Wales - Low TB
11
744
4.0%
9
1.2%
684
91.9%
65
8.7 %
51
6.9 %
0
Scotland - Scotland
Parallel New Infection
4
423
2.3%
5
1.2%
393
92.9%
31
7.3 %
23
5.4 %
2
0.5 %
Scotland - Scotland
4
423
2.3%
5
1.2%
393
92.9%
31
7.3 %
23
5.4 %
2
0.5 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
3
16
0.0%
0
0.0 %
15
93.8%
1
6.3 %
1
6.3 %
0
Extended Flexible IFN-GAMMA test
83
7815
6.2%
383
4.9%
7075
90.5%
424
5.4 %
314
4.0 %
43
0.6 %
Parallel – chronic breakdown
14
2334
1.9%
108
4.6%
2043
87.5%
222
9.5 %
182
7.8 %
1
0.0 %
Parallel - Herd or Group Slaughter
5
460
0.4%
36
7.8%
388
84.3%
53
11.5 %
36
7.8 %
0
Parallel - IRs in Exit Strategy for persistent herds
89
345
0.3%
104
30.1%
217
62.9%
26
7.5 %
23
6.7 %
1
0.3 %
Parallel New Infection
440
40454
32.1%
1192
2.9%
35774
88.4%
3299
8.2 %
2663
6.6 %
825
2.0 %
Parallel - new infection cull area
24
4340
3.4%
198
4.6%
3928
90.5%
250
5.8 %
198
4.6 %
16
0.4 %
Parallel - Other
40
2118
1.7%
134
6.3%
1814
85.6%
173
8.2 %
142
6.7 %
28
1.3 %
Parallel Persistent Infection
97
9915
7.9%
470
4.7%
8878
89.5%
647
6.5 %
511
5.2 %
56
0.6 %
Parallel - persistent infection cull area
1
539
0.4%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX anti
27
94
0.1%
11
11.7%
80
85.1%
5
5.3 %
3
3.2 %
0
Parallel - Rapid Testing Two-times IRs
194
474
0.4%
108
22.8%
343
72.4%
28
5.9 %
20
4.2 %
3
0.6 %
Parallel - Recurrent breakdowns
397
55747
44.2%
2353
4.2%
49479
88.8%
3904
7.0 %
3169
5.7 %
746
1.3 %
Parallel - severe IRs - in persistent herds
205
1440
1.1%
398
27.6%
959
66.6%
101
7.0 %
71
4.9 %
12
0.8 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Total
xx-xxxx-xxxx
100.0%
5526
4.4%
111489
88.4%
9167
7.3 %
7360
5.8 %
1732
1.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2023)
England - HRA
Parallel – chronic breakdown
14
2334
1.9%
108
4.6%
2043
87.5%
222
9.5 %
182
7.8 %
1
0.0 %
Parallel - Herd or Group Slaughter
3
457
0.4%
36
7.9%
385
84.2%
52
11.4 %
36
7.9 %
0
Parallel - new infection cull area
21
4136
3.3%
195
4.7%
3734
90.3%
243
5.9 %
191
4.6 %
16
0.4 %
Parallel - Other
12
940
0.7%
57
6.1%
802
85.3%
91
9.7 %
74
7.9 %
7
0.7 %
Parallel Persistent Infection
12
1787
1.4%
127
7.1%
1572
88.0%
106
5.9 %
88
4.9 %
0
Parallel - persistent infection cull area
1
539
0.4%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel - Recurrent breakdowns
288
46579
36.9%
2009
4.3%
41271
88.6%
3221
6.9 %
2621
5.6 %
678
1.5 %
England - Edge
Extended Flexible IFN-GAMMA test
2
55
0.0%
3
5.5%
48
87.3%
4
7.3 %
4
7.3 %
0
Parallel New Infection
101
10376
8.2%
288
2.8%
9379
90.4%
655
6.3 %
534
5.1 %
175
1.7 %
Parallel - new infection cull area
2
195
0.2%
3
1.5%
186
95.4%
6
3.1 %
6
3.1 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel - Other
4
535
0.4%
16
3.0%
489
91.4%
11
2.1 %
11
2.1 %
19
3.6 %
Parallel Persistent Infection
11
2163
1.7%
71
3.3%
1935
89.5%
184
8.5 %
157
7.3 %
0
Parallel - Recurrent breakdowns
41
5185
4.1%
149
2.9%
4738
91.4%
350
6.8 %
283
5.5 %
15
0.3 %
England - LRA
Parallel New Infection
46
5885
4.7%
129
2.2%
5017
85.3%
501
8.5 %
455
7.7 %
284
4.8 %
Parallel Persistent Infection
1
41
0.0%
0
0.0 %
41
100.0%
1
2.4 %
0
0
Parallel - Recurrent breakdowns
1
79
0.1%
2
2.5%
76
96.2%
3
3.8 %
1
1.3 %
0
England
560
81286
64.5%
3224
4.0%
72197
88.8%
5682
7.0 %
4669
5.7 %
1196
1.5 %
Wales - High TB
Anomalous reaction
3
16
0.0%
0
0.0 %
15
93.8%
1
6.3 %
1
6.3 %
0
Extended Flexible IFN-GAMMA test
73
7509
6.0%
362
4.8%
6814
90.7%
398
5.3 %
290
3.9 %
43
0.6 %
Parallel - Herd or Group Slaughter
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Parallel - IRs in Exit Strategy for persistent herd
63
275
0.2%
90
32.7%
167
60.7%
18
6.5 %
17
6.2 %
1
0.4 %
Parallel New Infection
2
152
0.1%
13
8.6%
131
86.2%
10
6.6 %
8
5.3 %
0
Parallel - Other
16
367
0.3%
15
4.1%
311
84.7%
53
14.4 %
40
10.9 %
1
0.3 %
Parallel Persistent Infection
60
5415
4.3%
220
4.1%
4914
90.7%
307
5.7 %
225
4.2 %
56
1.0 %
Parallel – post-mvt test coupled with IDEXX ant
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
159
406
0.3%
94
23.2%
292
71.9%
25
6.2 %
17
4.2 %
3
0.7 %
Parallel - Recurrent breakdowns
50
2889
2.3%
158
5.5%
2483
85.9%
247
8.5 %
201
7.0 %
47
1.6 %
Parallel - severe IRs - in persistent herds
177
1250
1.0%
359
28.7%
812
65.0%
97
7.8 %
67
5.4 %
12
1.0 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
86
0.1%
6
7.0%
75
87.2%
5
5.8 %
5
5.8 %
0
Parallel - IRs in Exit Strategy for persistent herd
23
62
0.0%
12
19.4%
44
71.0%
7
11.3 %
6
9.7 %
0
Parallel New Infection
69
5635
4.5%
130
2.3%
5104
90.6%
444
7.9 %
374
6.6 %
27
0.5 %
Parallel - Other
5
263
0.2%
45
17.1%
203
77.2%
15
5.7 %
14
5.3 %
1
0.4 %
Parallel Persistent Infection
12
502
0.4%
52
10.4%
410
81.7%
48
9.6 %
40
8.0 %
0
Parallel – post-mvt test coupled with IDEXX ant
12
65
0.1%
6
9.2%
56
86.2%
4
6.2 %
3
4.6 %
0
Parallel - Rapid Testing Two-times IRs
29
56
0.0%
13
23.2%
41
73.2%
2
3.6 %
2
3.6 %
0
Parallel - Recurrent breakdowns
12
724
0.6%
25
3.5%
644
89.0%
65
9.0 %
50
6.9 %
5
0.7 %
Parallel - severe IRs - in persistent herds
24
183
0.1%
35
19.1%
144
78.7%
4
2.2 %
4
2.2 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
165
0.1%
12
7.3%
138
83.6%
17
10.3 %
15
9.1 %
0
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - IRs in Exit Strategy for persistent herd
3
8
0.0%
2
25.0%
6
75.0%
1
12.5 %
0
0
Parallel New Infection
156
10957
8.7%
245
2.2%
9746
88.9%
1030
9.4 %
703
6.4 %
263
2.4 %
Parallel - Other
2
11
0.0%
1
9.1%
7
63.6%
3
27.3 %
3
27.3 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX ant
14
28
0.0%
5
17.9%
23
82.1%
1
3.6 %
0
0
Parallel - Rapid Testing Two-times IRs
6
12
0.0%
1
8.3%
10
83.3%
1
8.3 %
1
8.3 %
0
Parallel - Recurrent breakdowns
5
291
0.2%
10
3.4%
267
91.8%
18
6.2 %
13
4.5 %
1
0.3 %
Parallel - severe IRs - in persistent herds
4
7
0.0%
4
57.1%
3
42.9%
0
0
0
Wales
992
37359
29.6%
1915
5.1%
32883
88.0%
2824
7.6 %
2101
5.6 %
460
1.2 %
Scotland - Scotland
Parallel New Infection
66
7449
5.9%
387
5.2%
6397
85.9%
659
8.8 %
589
7.9 %
76
1.0 %
Parallel - new infection cull area
1
9
0.0%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Parallel - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Scotland
69
7462
5.9%
387
5.2%
6409
85.9%
661
8.9 %
590
7.9 %
76
1.0 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2022 August
274
19758
144
758
12
0
0
0
0
36
3
0
14
0
642
0
47
1
2
September
205
xx-xxxx-xxxx
107
672
13
0
0
0
0
100
0
1
7
0
520
0
31
0
0
October
180
xx-xxxx-xxxx
100
620
12
0
0
0
0
66
0
0
21
0
495
0
26
0
0
November
201
15848
120
673
16
0
0
0
0
60
1
1
13
0
504
1
77
0
0
December
200
13735
109
545
36
0
0
0
0
26
1
0
10
0
431
0
41
0
0
2023 January
281
23696
176
1138
10
0
0
0
0
83
3
0
18
0
922
1
100
1
0
February
265
17538
147
1036
6
0
0
0
0
69
1
4
31
0
837
0
88
0
0
March
266
19894
159
867
9
0
0
0
0
76
0
0
11
0
703
1
67
0
0
April
203
16194
118
782
1
13
0
0
0
0
53
0
1
10
6
631
0
58
1
4
May
208
13834
112
582
12
0
0
0
0
44
0
4
0
0
485
15
21
0
1
June
189
xx-xxxx-xxxx
94
524
61
0
0
0
0
27
0
11
0
0
397
27
0
0
1
July
209
18470
108
597
187
240
0
0
0
0
2
0
0
0
0
9
1
0
0
0
Totals (last
12 months)
2681
208288
1494
8794
188
440
0
0
0
0
642
9
22
135
6
6576
46
556
3
8
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
927
134122
636
5032
289
0
0
0
0
497
0
17
25
3
3922
1
19
0
0
Scotland
95
10866
60
491
33
0
0
0
0
117
2
1
31
0
273
2
25
0
0
Wales
1659
63300
798
3271
118
0
0
0
0
28
7
4
79
3
2381
43
512
3
8
Totals
2681
208288
1494
8794
440
0
0
0
0
642
9
22
135
6
6576
46
556
3
8
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
101
108
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2021
2022
2023
IFNg Positive herds
IFNg Negative herds
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2021
2022
2023
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2021
2022
2023
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
W Yorks
W Midlands
Wigtown
Staffs
Shropshire
Pembs
N Yorks
Mid Glamorgan
Kirkcudbright
Heref
Gwynedd
Gloucs
Dorset
Derbyshire
Corn & Scilly
Ceredigion
Bucks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during July 2023 (% submission per County)
County
%Resampled %Retested
Avon
2.65 %
3.46 %
Bucks
Carms
3.57 %
4.93 %
Ceredigion
3.23 %
3.23 %
Cheshire
Corn & Scilly
8.73 %
11.75 %
Cumbria
5.00 %
5.00 %
Derbyshire
3.96 %
5.74 %
Devon
2.77 %
4.42 %
Dorset
2.53 %
3.61 %
E Sussex
25.00 %
30.00 %
Gloucs
1.41 %
1.69 %
Gwent
6.99 %
13.99 %
Gwynedd
5.74 %
7.81 %
Hampshire
3.39 %
4.06 %
Heref
17.65 %
17.65 %
Herts
1.27 %
3.80 %
Kirkcudbright
4.27 %
5.28 %
Lincs
1.35 %
1.35 %
Mid Glamorgan
NE Wales
5.91 %
8.04 %
N Yorks
2.69 %
3.54 %
Oxon
4.32 %
6.29 %
Pembs
5.60 %
8.72 %
Powys
11.69 %
13.99 %
Shropshire
4.52 %
6.67 %
Somerset excl
1.95 %
2.28 %
Staffs
3.84 %
5.82 %
S Wales
Wigtown
24.00 %
40.00 %
Wiltshire
6.41 %
9.18 %
W Midlands
4.86 %
11.81 %
W Sussex
29.01 %
30.11 %
W Yorks
9.52 %
11.90 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
13445
449
3.3%
13479
449
3.3%
Wales
4499
143
3.2%
4568
143
3.1%
Scotland
411
5
1.2%
423
5
1.2%
GB
18355
597
3.3%
18470
597
3.2%
Table 6a (i): Number of animals and submissions tested in July 2023
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
77494
3224
4.2%
81286
3224
4.0%
Wales
35200
1915
5.4%
37359
1915
5.1%
Scotland
4399
387
8.8%
7462
387
5.2%
GB
117075
5526
4.7%
126107
5526
4.4%
Table 6b (i): Number of animals and submissions tested in 2023 Up to end of July
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
10090
299
3.0%
10211
299
2.9%
Wales
5100
186
3.6%
5191
186
3.6%
GB
15190
485
3.2%
15402
485
3.1%
Table 6a (ii): Number of animals and submissions tested in July 2022
Table 6b (ii): Number of animals and submissions tested in 2022 Up to end of July
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
94695
3742
4.0%
101050
3742
3.7%
Wales
28317
1842
6.5%
29905
1842
6.2%
Scotland
964
22
2.3%
1025
22
2.1%
GB
123974
5606
4.5%
131980
5606
4.2%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Herefordshire
PRV-SP
1
1
0
0
Somerset excl North
PRV-SE
1
1
0
0
PRV-SP
1
1
0
0
Total
3
3
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples July 2023
Table 7b Number of private samples 2023
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
4
27
0
0
Cumbria
PRV-SE
1
3
3
100.00 %
Devon
PRV-SE
5
102
10
9.80 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
51
2
3.92 %
Herefordshire
PRV-SP
1
1
0
0
Kent
PRV-SP
2
13
0
0
Northamptonshire
PRV-SE
1
6
3
50.00 %
Oxfordshire
PRV-SP
2
2
0
0
Shropshire
PRV-SP
2
2
0
0
Somerset excl North
PRV-SE
1
1
0
0
PRV-SP
1
1
0
0
West Sussex
PRV-SE
4
553
24
4.34 %
PRV-SP
1
5
0
0
Total
27
769
42
5.46 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Somerset excl North
BOV-P
1
145
6
4.1%
England
1
145
6
4.1%
Wales
Carmarthenshire
BOV-IR-C
3
30
8
26.7%
Ceredigion
BOV-IR-C
1
8
2
25.0%
North East Wales
BOV-IR-EX
2
12
0
0
Pembrokeshire
BOV-IR-C
7
76
14
18.4%
BOV-IR-EX
2
22
4
18.2%
BOV-OTHER
2
94
4
4.3%
BOV-P
2
83
8
9.6%
Powys
BOV-IR-C
1
9
0
0
South Wales
BOV-IR-EX
2
14
6
42.9%
BOV-P
2
69
10
14.5%
Wales
24
417
56
13.4%
All GB
25
562
62
11.0 %
Table 8a Number of Cattle Antibody Tests July 2023
Table 8b Number of Cattle Antibody Tests 2023
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
569
38
6.7%
Northern Somerset &
South
Gloucestershire
BOV-P
1
5
0
0
Somerset excl North
BOV-P
1
145
6
4.1%
England
4
719
44
6.1%
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
24
192
54
28.1%
BOV-IR-EX
6
36
5
13.9%
BOV-OTHER
1
34
9
26.5%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
19
183
28
15.3%
BOV-IR-EX
3
25
8
32.0%
BOV-OTHER
2
113
3
2.7%
North East Wales
BOV-IR-C
16
137
27
19.7%
BOV-IR-EX
5
26
0
0
BOV-OTHER
1
54
0
0
BOV-P
2
67
2
3.0%
BOV-POSTM
8
21
5
23.8%
Pembrokeshire
BOV-IR-C
89
796
143
18.0%
BOV-IR-EX
19
131
22
16.8%
BOV-OTHER
9
538
24
4.5%
BOV-P
4
130
10
7.7%
Powys
1
8
1
12.5%
BOV-IR-C
25
140
20
14.3%
BOV-IR-EX
1
8
0
0
BOV-OTHER
1
113
5
4.4%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
South Wales
BOV-IR-C
6
44
11
25.0%
BOV-IR-EX
4
28
12
42.9%
BOV-OTHER
1
30
1
3.3%
BOV-P
5
255
16
6.3%
Wales
253
3,158
407
12.9%
All GB
258
3,879
452
11.7 %