Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - February 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 174
Report Period 1st - 28th February 2021
Issued 10 March 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
Parallel blood testing of skin test-negative cattle
in breakdowns herds occurring within 6 months
of lifting restrictions after a previous breakdown
NO (APHA discretion -
Wales only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Test Criteria

Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
February
2021
%
Total 2021
%
Submissions
352
727
Samples
Total (%)
28759
100.00 %
56699
100.00 %
England
22216
77.25 %
45341
79.97 %
Wales
6142
21.36 %
10937
19.29 %
Scotland
401
1.39 %
421
0.74 %
Parallel Tests
PAR-CUL-N
13529
47.04 %
24874
43.87 %
PAR-CUL-P
83
0.29 %
900
1.59 %
PAR-HERD-S
1000
3.48 %
1000
1.76 %
PAR-IR-EX
50
0.17 %
50
0.09 %
PAR-IR-SEV
191
0.66 %
191
0.34 %
PAR-NEW-IN
10885
37.85 %
22501
39.69 %
PAR-OTHER
1085
3.77 %
2269
4.00 %
PAR-PERSIS
993
3.45 %
3637
6.41 %
PAR-RAPID
101
0.35 %
286
0.50 %
PAR-RECUR
424
1.47 %
424
0.75 %
Total(% of all samples)
28341
98.55 %
56132
99.00 %
Serial/Extended Tests
SER-FLEXI
366
1.27 %
515
0.91 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
SER-OTHER
52
0.18 %
52
0.09 %
Total(% of all samples)
418
1.45 %
567
1.00 %
Total
28759
100.00 %
56699
100.00 %
Retests
Total (% of all samples)
2319
8.06 %
4277
7.54 %
England
1815
6.31 %
3417
6.03 %
Wales
465
1.62 %
819
1.44 %
Scotland
39
0.14 %
41
0.07 %
Resamples
Total (% of all samples)
2088
7.26 %
3777
6.66 %
England
1652
5.74 %
3046
5.37 %
Wales
399
1.39 %
692
1.22 %
Scotland
37
0.13 %
39
0.07 %
Rejects
Total (% of all samples)
152
0.53 %
229
0.40 %
England
129
0.45 %
171
0.30 %
Wales
16
0.06 %
51
0.09 %
Scotland
7
0.02 %
7
0.01 %
Table 1. Monthly Totals for February 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-OTHER
Breakdown of
Reason for
submission
PAR-CUL-N
13529
47.0 %
PAR-CUL-P
83
0.3 %
PAR-HERD-S
1000
3.5 %
PAR-IR-EX
50
0.2 %
PAR-IR-SEV
191
0.7 %
PAR-NEW-IN
10885
37.8 %
PAR-OTHER
1085
3.8 %
PAR-PERSIS
993
3.5 %
PAR-RAPID
101
0.4 %
PAR-RECUR
424
1.5 %
SER-FLEXI
366
1.3 %
SER-OTHER
52
0.2 %
Total
28759
100.00 %
Figure 1. Number of samples submitted in each category during February 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Ayrshire
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northants
Lincs
Leics & Rut
Herts
Heref
Hampshire
Gr Manchester
Gloucs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during February 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
2000
4000
6000
8000
10000
12000
Lanarkshire
Dumfries & G
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
3
61
0.21 %
10
16.39 %
33
54.10 %
18
29.51 %
18
29.51 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
15
1,922
6.68 %
90
4.68 %
1566
81.48 %
281
14.62 %
257
13.37 %
9
0.47 %
PAR-CUL-P
2
61
0.21 %
1
1.64 %
53
86.89 %
7
11.48 %
7
11.48 %
0
0.00 %
Devon
PAR-CUL-N
39
4,723
xx-xxxx-xxxx %
331
7.01 %
4083
86.45 %
294
6.22 %
271
5.74 %
38
0.80 %
PAR-NEW-IN
6
591
2.06 %
26
4.40 %
533
90.19 %
31
5.25 %
29
4.91 %
3
0.51 %
Dorset
PAR-CUL-N
10
886
3.08 %
100
11.29 %
739
83.41 %
54
6.09 %
47
5.30 %
0
0.00 %
Gloucs
PAR-CUL-N
10
605
2.10 %
23
3.80 %
494
81.65 %
68
11.24 %
64
10.58 %
24
3.97 %
Heref
PAR-CUL-N
4
249
0.87 %
33
13.25 %
175
70.28 %
22
8.84 %
22
8.84 %
19
7.63 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
10
0.03 %
1
10.00 %
5
50.00 %
4
40.00 %
4
40.00 %
0
0.00 %
SER-OTHER
1
52
0.18 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
5
2,062
7.17 %
68
3.30 %
1868
90.59 %
133
6.45 %
123
5.97 %
3
0.15 %
Somerset excl N
PAR-CUL-N
9
376
1.31 %
33
8.78 %
331
88.03 %
25
6.65 %
12
3.19 %
0
0.00 %
PAR-NEW-IN
2
370
1.29 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
1
49
0.17 %
1
2.04 %
41
83.67 %
8
16.33 %
7
14.29 %
0
0.00 %
Staffs
PAR-CUL-N
8
1,040
3.62 %
40
3.85 %
917
88.17 %
89
8.56 %
82
7.88 %
1
0.10 %
PAR-CUL-P
2
22
0.08 %
2
9.09 %
15
68.18 %
5
22.73 %
5
22.73 %
0
0.00 %
Wiltshire
PAR-CUL-N
6
200
0.70 %
10
5.00 %
168
84.00 %
18
9.00 %
18
9.00 %
4
2.00 %
PAR-NEW-IN
1
9
0.03 %
1
11.11 %
4
44.44 %
4
44.44 %
4
44.44 %
0
0.00 %
Worcs
PAR-CUL-N
1
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
237
0.82 %
7
2.95 %
216
91.14 %
17
7.17 %
14
5.91 %
0
0.00 %
Bucks
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-CUL-N
7
437
1.52 %
14
3.20 %
407
93.14 %
17
3.89 %
15
3.43 %
1
0.23 %
PAR-NEW-IN
5
549
1.91 %
11
2.00 %
514
93.62 %
29
5.28 %
24
4.37 %
0
0.00 %
PAR-PERSIS
2
14
0.05 %
1
7.14 %
9
64.29 %
3
21.43 %
3
21.43 %
1
7.14 %
Derbyshire
PAR-CUL-N
3
790
2.75 %
41
5.19 %
702
88.86 %
50
6.33 %
47
5.95 %
0
0.00 %
Table 2a. Summary by County for February 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
15
3,117
10.84 %
143
4.59 %
2648
84.95 %
349
11.20 %
309
9.91 %
17
0.55 %
Hampshire
PAR-CUL-N
2
217
0.75 %
14
6.45 %
199
91.71 %
5
2.30 %
4
1.84 %
0
0.00 %
PAR-NEW-IN
2
231
0.80 %
11
4.76 %
214
92.64 %
6
2.60 %
6
2.60 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
8
884
3.07 %
59
6.67 %
763
86.31 %
71
8.03 %
62
7.01 %
0
0.00 %
Northants
PAR-NEW-IN
4
171
0.59 %
6
3.51 %
146
85.38 %
21
12.28 %
19
11.11 %
0
0.00 %
PAR-PERSIS
1
168
0.58 %
2
1.19 %
156
92.86 %
11
6.55 %
10
5.95 %
0
0.00 %
Oxon
PAR-NEW-IN
7
441
1.53 %
28
6.35 %
375
85.03 %
40
9.07 %
38
8.62 %
0
0.00 %
PAR-PERSIS
1
80
0.28 %
4
5.00 %
71
88.75 %
5
6.25 %
5
6.25 %
0
0.00 %
Warks
PAR-NEW-IN
12
1,040
3.62 %
41
3.94 %
940
90.38 %
59
5.67 %
56
5.38 %
3
0.29 %
England - LRA
Cumbria
PAR-CUL-N
1
18
0.06 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
Gr Manchester
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Herts
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Lincs
PAR-NEW-IN
2
182
0.63 %
4
2.20 %
165
90.66 %
12
6.59 %
12
6.59 %
1
0.55 %
N Yorks
PAR-NEW-IN
2
167
0.58 %
0
0.00 %
159
95.21 %
8
4.79 %
7
4.19 %
1
0.60 %
S Yorks
PAR-NEW-IN
1
176
0.61 %
8
4.55 %
139
78.98 %
27
15.34 %
27
15.34 %
2
1.14 %
England
208
22216
77.25 %
1205
5.42 %
19230
86.56 %
1815
8.17 %
1652
7.44 %
129
0.58 %
Wales - High TB
Carms
PAR-IR-SEV
5
30
0.10 %
10
33.33 %
17
56.67 %
4
13.33 %
3
10.00 %
0
0.00 %
PAR-OTHER
4
121
0.42 %
9
7.44 %
111
91.74 %
2
1.65 %
1
0.83 %
0
0.00 %
PAR-PERSIS
4
128
0.45 %
13
10.16 %
110
85.94 %
12
9.38 %
5
3.91 %
0
0.00 %
PAR-RAPID
9
19
0.07 %
7
36.84 %
12
63.16 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
23
0.08 %
7
30.43 %
15
65.22 %
1
4.35 %
1
4.35 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
18
0.06 %
7
38.89 %
11
61.11 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
39
0.14 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
1
5
0.02 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
13
0.05 %
2
15.38 %
11
84.62 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Gwent
PAR-OTHER
2
33
0.11 %
3
9.09 %
30
90.91 %
3
9.09 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Pembs
PAR-IR-EX
3
13
0.05 %
1
7.69 %
12
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
10
120
0.42 %
32
26.67 %
84
70.00 %
5
4.17 %
4
3.33 %
0
0.00 %
PAR-OTHER
10
269
0.94 %
15
5.58 %
235
87.36 %
19
7.06 %
18
6.69 %
1
0.37 %
PAR-PERSIS
6
160
0.56 %
3
1.88 %
149
93.13 %
8
5.00 %
8
5.00 %
0
0.00 %
PAR-RAPID
21
54
0.19 %
15
27.78 %
38
70.37 %
1
1.85 %
1
1.85 %
0
0.00 %
PAR-RECUR
2
330
1.15 %
18
5.45 %
299
90.61 %
14
4.24 %
13
3.94 %
0
0.00 %
SER-FLEXI
4
226
0.79 %
12
5.31 %
209
92.48 %
8
3.54 %
5
2.21 %
0
0.00 %
Powys
PAR-IR-EX
1
32
0.11 %
14
43.75 %
15
46.88 %
4
12.50 %
3
9.38 %
0
0.00 %
PAR-IR-SEV
2
22
0.08 %
2
9.09 %
20
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
327
1.14 %
13
3.98 %
293
89.60 %
20
6.12 %
17
5.20 %
4
1.22 %
PAR-PERSIS
3
166
0.58 %
10
6.02 %
143
86.14 %
13
7.83 %
12
7.23 %
1
0.60 %
PAR-RAPID
2
12
0.04 %
1
8.33 %
11
91.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
74
0.26 %
13
17.57 %
55
74.32 %
7
9.46 %
6
8.11 %
0
0.00 %
S Wales
PAR-HERD-S
2
992
3.45 %
43
4.33 %
909
91.63 %
41
4.13 %
35
3.53 %
5
0.50 %
Wales - Intermediate TB
Carms
PAR-HERD-S
1
8
0.03 %
1
12.50 %
6
75.00 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-PERSIS
1
173
0.60 %
13
7.51 %
147
84.97 %
15
8.67 %
13
7.51 %
0
0.00 %
SER-FLEXI
1
43
0.15 %
9
20.93 %
33
76.74 %
3
6.98 %
1
2.33 %
0
0.00 %
Ceredigion
PAR-OTHER
1
101
0.35 %
6
5.94 %
90
89.11 %
8
7.92 %
4
3.96 %
1
0.99 %
NE Wales
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
15
1,375
4.78 %
59
4.29 %
1192
86.69 %
131
9.53 %
122
8.87 %
2
0.15 %
PAR-PERSIS
1
14
0.05 %
0
0.00 %
11
78.57 %
3
21.43 %
3
21.43 %
0
0.00 %
Powys
PAR-NEW-IN
1
200
0.70 %
8
4.00 %
164
82.00 %
32
16.00 %
28
14.00 %
0
0.00 %
PAR-PERSIS
1
14
0.05 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-RECUR
1
89
0.31 %
6
6.74 %
79
88.76 %
6
6.74 %
3
3.37 %
1
1.12 %
S Glamorgan
PAR-RAPID
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
2
16
0.06 %
3
18.75 %
9
56.25 %
4
25.00 %
4
25.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
4
75
0.26 %
2
2.67 %
65
86.67 %
8
10.67 %
8
10.67 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
NE Wales
PAR-NEW-IN
4
649
2.26 %
25
3.85 %
559
86.13 %
75
11.56 %
65
10.02 %
0
0.00 %
PAR-OTHER
1
145
0.50 %
4
2.76 %
135
93.10 %
6
4.14 %
5
3.45 %
1
0.69 %
Wales
141
6142
21.36 %
398
6.48 %
5329
86.76 %
465
7.57 %
399
6.50 %
16
0.26 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
401
1.39 %
34
8.48 %
323
80.55 %
39
9.73 %
37
9.23 %
7
1.75 %
Scotland
3
401
1.39 %
34
8.48 %
323
80.55 %
39
9.73 %
37
9.23 %
7
1.75 %
Grand Total
Sum:
352
28759
1637
24882
2319
2088
152
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
6
719
1.27 %
131
18.22 %
509
70.79 %
79
10.99 %
70
9.74 %
9
1.25 %
Corn & Scilly
PAR-CUL-N
23
2,400
4.23 %
112
4.67 %
1987
82.79 %
323
13.46 %
291
12.13 %
10
0.42 %
PAR-CUL-P
5
868
1.53 %
34
3.92 %
767
88.36 %
70
8.06 %
65
7.49 %
2
0.23 %
Devon
PAR-CUL-N
73
9,499
16.75 %
679
7.15 %
8274
87.10 %
543
5.72 %
496
5.22 %
50
0.53 %
PAR-CUL-P
1
6
0.01 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-NEW-IN
6
591
1.04 %
26
4.40 %
533
90.19 %
31
5.25 %
29
4.91 %
3
0.51 %
Dorset
PAR-CUL-N
19
1,602
2.83 %
133
8.30 %
1387
86.58 %
91
5.68 %
80
4.99 %
2
0.12 %
Gloucs
PAR-CUL-N
17
1,480
2.61 %
92
6.22 %
1240
83.78 %
141
9.53 %
124
8.38 %
24
1.62 %
Heref
PAR-CUL-N
6
362
0.64 %
39
10.77 %
273
75.41 %
31
8.56 %
31
8.56 %
19
5.25 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
110
0.19 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
SER-OTHER
1
52
0.09 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
13
3,107
5.48 %
110
3.54 %
2819
90.73 %
191
6.15 %
174
5.60 %
4
0.13 %
Somerset excl N
PAR-CUL-N
17
1,189
2.10 %
76
6.39 %
1075
90.41 %
58
4.88 %
38
3.20 %
0
0.00 %
PAR-NEW-IN
2
370
0.65 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.82 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
Staffs
PAR-CUL-N
17
2,236
3.94 %
119
5.32 %
1933
86.45 %
200
8.94 %
183
8.18 %
1
0.04 %
PAR-CUL-P
3
26
0.05 %
2
7.69 %
18
69.23 %
6
23.08 %
6
23.08 %
0
0.00 %
Wiltshire
PAR-CUL-N
15
1,409
2.49 %
114
8.09 %
1203
85.38 %
108
7.67 %
88
6.25 %
4
0.28 %
PAR-NEW-IN
2
191
0.34 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
1
23
0.04 %
3
13.04 %
14
60.87 %
7
30.43 %
6
26.09 %
0
0.00 %
Worcs
PAR-CUL-N
2
128
0.23 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
England - Edge
Berks
PAR-NEW-IN
5
346
0.61 %
21
6.07 %
296
85.55 %
33
9.54 %
29
8.38 %
0
0.00 %
Bucks
PAR-NEW-IN
2
80
0.14 %
4
5.00 %
74
92.50 %
2
2.50 %
2
2.50 %
0
0.00 %
Cheshire
PAR-CUL-N
7
437
0.77 %
14
3.20 %
407
93.14 %
17
3.89 %
15
3.43 %
1
0.23 %
PAR-NEW-IN
12
1,397
2.46 %
56
4.01 %
1280
91.62 %
79
5.65 %
61
4.37 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
14
0.02 %
1
7.14 %
9
64.29 %
3
21.43 %
3
21.43 %
1
7.14 %
Derbyshire
PAR-CUL-N
3
790
1.39 %
41
5.19 %
702
88.86 %
50
6.33 %
47
5.95 %
0
0.00 %
PAR-NEW-IN
28
4,159
7.34 %
194
4.66 %
3545
85.24 %
468
11.25 %
403
9.69 %
17
0.41 %
E Sussex
PAR-NEW-IN
1
18
0.03 %
3
16.67 %
11
61.11 %
5
27.78 %
4
22.22 %
0
0.00 %
Hampshire
PAR-CUL-N
2
217
0.38 %
14
6.45 %
199
91.71 %
5
2.30 %
4
1.84 %
0
0.00 %
PAR-NEW-IN
8
1,592
2.81 %
54
3.39 %
1415
88.88 %
132
8.29 %
121
7.60 %
2
0.13 %
PAR-PERSIS
1
76
0.13 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
14
1,647
2.90 %
80
4.86 %
1485
90.16 %
101
6.13 %
82
4.98 %
0
0.00 %
Northants
PAR-NEW-IN
13
827
1.46 %
32
3.87 %
738
89.24 %
63
7.62 %
57
6.89 %
0
0.00 %
PAR-PERSIS
1
168
0.30 %
2
1.19 %
156
92.86 %
11
6.55 %
10
5.95 %
0
0.00 %
Notts
PAR-NEW-IN
1
12
0.02 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Oxon
PAR-NEW-IN
23
1,996
3.52 %
74
3.71 %
1792
89.78 %
140
7.01 %
126
6.31 %
4
0.20 %
PAR-PERSIS
1
80
0.14 %
4
5.00 %
71
88.75 %
5
6.25 %
5
6.25 %
0
0.00 %
Warks
PAR-NEW-IN
22
1,720
3.03 %
71
4.13 %
1528
88.84 %
124
7.21 %
111
6.45 %
10
0.58 %
England - LRA
Cleveland
PAR-NEW-IN
1
186
0.33 %
5
2.69 %
173
93.01 %
10
5.38 %
8
4.30 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.03 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
8
1,340
2.36 %
42
3.13 %
1246
92.99 %
70
5.22 %
52
3.88 %
0
0.00 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.17 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
3
42
0.07 %
2
4.76 %
38
90.48 %
3
7.14 %
2
4.76 %
0
0.00 %
Herts
PAR-NEW-IN
2
92
0.16 %
9
9.78 %
82
89.13 %
1
1.09 %
1
1.09 %
0
0.00 %
Lancashire
PAR-NEW-IN
2
42
0.07 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Lincs
PAR-NEW-IN
6
526
0.93 %
21
3.99 %
473
89.92 %
36
6.84 %
31
5.89 %
1
0.19 %
N Yorks
PAR-NEW-IN
9
413
0.73 %
26
6.30 %
347
84.02 %
44
10.65 %
39
9.44 %
1
0.24 %
S Yorks
PAR-NEW-IN
1
176
0.31 %
8
4.55 %
139
78.98 %
27
15.34 %
27
15.34 %
2
1.14 %
England
414
45341
79.97 %
2559
5.64 %
39565
87.26 %
3417
7.54 %
3046
6.72 %
171
0.38 %
Wales - High TB
Carms
PAR-IR-SEV
5
30
0.05 %
10
33.33 %
17
56.67 %
4
13.33 %
3
10.00 %
0
0.00 %
PAR-NEW-IN
1
6
0.01 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
9
272
0.48 %
24
8.82 %
229
84.19 %
19
6.99 %
17
6.25 %
2
0.74 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
7
139
0.25 %
16
11.51 %
118
84.89 %
12
8.63 %
5
3.60 %
0
0.00 %
PAR-RAPID
20
34
0.06 %
9
26.47 %
25
73.53 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
23
0.04 %
7
30.43 %
15
65.22 %
1
4.35 %
1
4.35 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
18
0.03 %
7
38.89 %
11
61.11 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
39
0.07 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
6
481
0.85 %
37
7.69 %
411
85.45 %
29
6.03 %
20
4.16 %
13
2.70 %
PAR-RAPID
11
34
0.06 %
7
20.59 %
26
76.47 %
2
5.88 %
1
2.94 %
0
0.00 %
PAR-RECUR
1
5
0.01 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Gwent
PAR-OTHER
2
33
0.06 %
3
9.09 %
30
90.91 %
3
9.09 %
0
0.00 %
0
0.00 %
PAR-PERSIS
7
98
0.17 %
7
7.14 %
85
86.73 %
5
5.10 %
5
5.10 %
1
1.02 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
3
13
0.02 %
1
7.69 %
12
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
10
120
0.21 %
32
26.67 %
84
70.00 %
5
4.17 %
4
3.33 %
0
0.00 %
PAR-OTHER
21
833
1.47 %
43
5.16 %
746
89.56 %
49
5.88 %
41
4.92 %
3
0.36 %
PAR-PERSIS
28
345
0.61 %
43
12.46 %
286
82.90 %
18
5.22 %
16
4.64 %
0
0.00 %
PAR-RAPID
46
180
0.32 %
47
26.11 %
129
71.67 %
5
2.78 %
4
2.22 %
0
0.00 %
PAR-RECUR
2
330
0.58 %
18
5.45 %
299
90.61 %
14
4.24 %
13
3.94 %
0
0.00 %
SER-FLEXI
4
226
0.40 %
12
5.31 %
209
92.48 %
8
3.54 %
5
2.21 %
0
0.00 %
Powys
PAR-IR-EX
1
32
0.06 %
14
43.75 %
15
46.88 %
4
12.50 %
3
9.38 %
0
0.00 %
PAR-IR-SEV
2
22
0.04 %
2
9.09 %
20
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
239
0.42 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
5
374
0.66 %
17
4.55 %
336
89.84 %
22
5.88 %
17
4.55 %
4
1.07 %
PAR-PERSIS
21
898
1.58 %
29
3.23 %
790
87.97 %
76
8.46 %
71
7.91 %
8
0.89 %
PAR-RAPID
4
14
0.02 %
1
7.14 %
13
92.86 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
75
0.13 %
13
17.33 %
56
74.67 %
7
9.33 %
6
8.00 %
0
0.00 %
S Wales
PAR-HERD-S
2
992
1.75 %
43
4.33 %
909
91.63 %
41
4.13 %
35
3.53 %
5
0.50 %
W Glamorgan
PAR-PERSIS
2
136
0.24 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-HERD-S
1
8
0.01 %
1
12.50 %
6
75.00 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-PERSIS
3
176
0.31 %
15
8.52 %
148
84.09 %
15
8.52 %
13
7.39 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
43
0.08 %
9
20.93 %
33
76.74 %
3
6.98 %
1
2.33 %
0
0.00 %
Ceredigion
PAR-OTHER
1
101
0.18 %
6
5.94 %
90
89.11 %
8
7.92 %
4
3.96 %
1
0.99 %
SER-FLEXI
3
148
0.26 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
NE Wales
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
24
1,738
3.07 %
71
4.09 %
1521
87.51 %
157
9.03 %
144
8.29 %
2
0.12 %
PAR-OTHER
2
3
0.01 %
0
0.00 %
1
33.33 %
2
66.67 %
2
66.67 %
0
0.00 %
PAR-PERSIS
3
160
0.28 %
1
0.63 %
154
96.25 %
7
4.38 %
5
3.13 %
0
0.00 %
PAR-RAPID
1
19
0.03 %
3
15.79 %
15
78.95 %
1
5.26 %
1
5.26 %
0
0.00 %
Powys
PAR-NEW-IN
4
390
0.69 %
12
3.08 %
335
85.90 %
49
12.56 %
42
10.77 %
1
0.26 %
PAR-PERSIS
1
14
0.02 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-RECUR
1
89
0.16 %
6
6.74 %
79
88.76 %
6
6.74 %
3
3.37 %
1
1.12 %
S Glamorgan
PAR-RAPID
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
126
0.22 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
10
300
0.53 %
12
4.00 %
257
85.67 %
40
13.33 %
31
10.33 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
14
1,424
2.51 %
84
5.90 %
1218
85.53 %
143
10.04 %
119
8.36 %
3
0.21 %
PAR-OTHER
2
146
0.26 %
4
2.74 %
136
93.15 %
6
4.11 %
5
3.42 %
1
0.68 %
Wales
308
10937
19.29 %
696
6.36 %
9498
86.84 %
819
7.49 %
692
6.33 %
51
0.47 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
401
0.71 %
34
8.48 %
323
80.55 %
39
9.73 %
37
9.23 %
7
1.75 %
Dumfries & G
PAR-NEW-IN
1
8
0.01 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.02 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
5
421
0.74 %
35
8.31 %
340
80.76 %
41
9.74 %
39
9.26 %
7
1.66 %
Grand Total
Sum:
727
56699
3290
49403
4277
3777
229
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol February 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
207
22164
99.8%
1205
5.4%
19179
86.5%
1814
8.2%
1651
7.4%
129
0.6%
Serial/Extended
1
52
0.2%
0
0.0 %
51
98.1%
1
1.9%
1
1.9%
0
0.0 %
Total
208
22216
100.0%
1205
5.4%
19230
86.6%
1815
8.2%
1652
7.4%
129
0.6%
Scotland
Parallel
3
401
100.0%
34
8.5%
323
80.5%
39
9.7%
37
9.2%
7
1.7%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
3
401
100.0%
34
8.5%
323
80.5%
39
9.7%
37
9.2%
7
1.7%
Wales
Parallel
134
5776
94.0%
357
6.2%
5017
86.9%
446
7.7%
386
6.7%
16
0.3%
Serial/Extended
7
366
6.0%
41
11.2%
312
85.2%
19
5.2%
13
3.6%
0
0.0 %
Total
141
6142
100.0%
398
6.5%
5329
86.8%
465
7.6%
399
6.5%
16
0.3%
GB
Parallel
344
28341
98.5%
1596
5.6%
24519
86.5%
2299
8.1%
2074
7.3%
152
0.5%
Serial/Extended
8
418
1.5%
41
9.8%
363
86.8%
20
4.8%
14
3.3%
0
0.0 %
Total
352
28759
100.0%
1637
5.7%
24882
86.5%
2319
8.1%
2088
7.3%
152
0.5%
208.00
141.00
3.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
413
45289
99.9%
2559
5.7%
39514
87.2%
3416
7.5%
3045
6.7%
171
0.4%
Serial/Extended
1
52
0.1%
0
0.0 %
51
98.1%
1
1.9%
1
1.9%
0
0.0 %
Total
414
45341
100.0%
2559
5.6%
39565
87.3%
3417
7.5%
3046
6.7%
171
0.4%
Scotland
Parallel
5
421
100.0%
35
8.3%
340
80.8%
41
9.7%
39
9.3%
7
1.7%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
5
421
100.0%
35
8.3%
340
80.8%
41
9.7%
39
9.3%
7
1.7%
Wales
Parallel
297
10422
95.3%
649
6.2%
9046
86.8%
797
7.6%
677
6.5%
50
0.5%
Serial/Extended
11
515
4.7%
47
9.1%
452
87.8%
22
4.3%
15
2.9%
1
0.2%
Total
308
10937
100.0%
696
6.4%
9498
86.8%
819
7.5%
692
6.3%
51
0.5%
GB
Parallel
715
56132
99.0%
3243
5.8%
48900
87.1%
4254
7.6%
3761
6.7%
228
0.4%
Serial/Extended
12
567
1.0%
47
8.3%
503
88.7%
23
4.1%
16
2.8%
1
0.2%
Total
727
56699
100.0%
3290
5.8%
49403
87.1%
4277
7.5%
3777
6.7%
229
0.4%
414.00
308.00
5.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
7
366
1.3%
41
11.2%
312
85.2%
19
5.2 %
13
3.6 %
0
Parallel - Herd or Group Slaughter
3
1000
3.5%
44
4.4%
915
91.5%
42
4.2 %
36
3.6 %
5
0.5 %
Parallel New Infection
102
10885
37.8%
516
4.7%
9449
86.8%
976
9.0 %
884
8.1 %
36
0.3 %
Parallel - new infection cull area
120
13529
47.0%
798
5.9%
11658
86.2%
1066
7.9 %
972
7.2 %
101
0.7 %
Parallel - Other
24
1085
3.8%
54
5.0%
967
89.1%
72
6.6 %
57
5.3 %
7
0.6 %
Parallel Persistent Infection
25
993
3.5%
62
6.2%
844
85.0%
96
9.7 %
85
8.6 %
2
0.2 %
Parallel - persistent infection cull area
4
83
0.3%
3
3.6%
68
81.9%
12
14.5 %
12
14.5 %
0
Parallel - Rapid Testing Two-times IRs
37
101
0.4%
28
27.7%
72
71.3%
1
1.0 %
1
1.0 %
0
PAR-IR-EX
5
50
0.2%
15
30.0%
32
64.0%
4
8.0 %
3
6.0 %
0
PAR-IR-SEV
20
191
0.7%
51
26.7%
133
69.6%
9
4.7 %
7
3.7 %
0
PAR-RECUR
4
424
1.5%
25
5.9%
381
89.9%
21
5.0 %
17
4.0 %
1
0.2 %
Serial - Other
1
52
0.2%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Total
352
28759
100.0%
1637
5.7%
24882
86.5%
2319
8.1 %
2088
7.3 %
152
0.5 %
Table 4a. Summaryof IFN Gamma results by submission reason (February 2021)
England - HRA
Parallel New Infection
9
970
3.4%
67
6.9%
854
88.0%
48
4.9 %
46
4.7 %
3
0.3 %
Parallel - new infection cull area
107
12067
42.0%
729
6.0%
10344
85.7%
984
8.2 %
896
7.4 %
98
0.8 %
Parallel - Other
2
50
0.2%
1
2.0%
42
84.0%
8
16.0 %
7
14.0 %
0
Parallel Persistent Infection
4
71
0.2%
11
15.5%
38
53.5%
22
31.0 %
22
31.0 %
0
Parallel - persistent infection cull area
4
83
0.3%
3
3.6%
68
81.9%
12
14.5 %
12
14.5 %
0
Serial - Other
1
52
0.2%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - HRA
127
13293
46.2%
811
6.1%
11397
85.7%
1075
8.1 %
984
7.4 %
101
0.8 %
England - Edge
Parallel New Infection
57
6672
23.2%
306
4.6%
5818
87.2%
592
8.9 %
528
7.9 %
20
0.3 %
Parallel - new infection cull area
12
1444
5.0%
69
4.8%
1308
90.6%
72
5.0 %
66
4.6 %
1
0.1 %
Parallel Persistent Infection
4
262
0.9%
7
2.7%
236
90.1%
19
7.3 %
18
6.9 %
1
0.4 %
England - Edge
73
8378
29.1%
382
4.6%
7362
87.9%
683
8.2 %
612
7.3 %
22
0.3 %
England - LRA
Parallel New Infection
7
527
1.8%
12
2.3%
465
88.2%
47
8.9 %
46
8.7 %
4
0.8 %
Parallel - new infection cull area
1
18
0.1%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England - LRA
8
545
1.9%
12
2.2%
471
86.4%
57
10.5 %
56
10.3 %
6
1.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
6
323
1.1%
32
9.9%
279
86.4%
16
5.0 %
12
3.7 %
0
Parallel - Herd or Group Slaughter
2
992
3.4%
43
4.3%
909
91.6%
41
4.1 %
35
3.5 %
5
0.5 %
Parallel - Other
20
789
2.7%
43
5.4%
700
88.7%
50
6.3 %
41
5.2 %
5
0.6 %
Parallel Persistent Infection
14
459
1.6%
29
6.3%
404
88.0%
33
7.2 %
25
5.4 %
1
0.2 %
Parallel - Rapid Testing Two-times IRs
35
98
0.3%
25
25.5%
72
73.5%
1
1.0 %
1
1.0 %
0
PAR-IR-EX
5
50
0.2%
15
30.0%
32
64.0%
4
8.0 %
3
6.0 %
0
PAR-IR-SEV
19
190
0.7%
51
26.8%
132
69.5%
9
4.7 %
7
3.7 %
0
PAR-RECUR
3
335
1.2%
19
5.7%
302
90.1%
15
4.5 %
14
4.2 %
0
Wales - High TB
104
3236
11.3%
257
7.9%
2830
87.5%
169
5.2 %
138
4.3 %
11
0.3 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
43
0.1%
9
20.9%
33
76.7%
3
7.0 %
1
2.3 %
0
Parallel - Herd or Group Slaughter
1
8
0.0%
1
12.5%
6
75.0%
1
12.5 %
1
12.5 %
0
Parallel New Infection
18
1591
5.5%
70
4.4%
1365
85.8%
167
10.5 %
154
9.7 %
2
0.1 %
Parallel - Other
1
101
0.4%
6
5.9%
90
89.1%
8
7.9 %
4
4.0 %
1
1.0 %
Parallel Persistent Infection
3
201
0.7%
15
7.5%
166
82.6%
22
10.9 %
20
10.0 %
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
2
100.0%
0
0.0 %
0
0
0
PAR-IR-SEV
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
PAR-RECUR
1
89
0.3%
6
6.7%
79
88.8%
6
6.7 %
3
3.4 %
1
1.1 %
Wales - Intermediate TB
27
2036
7.1%
109
5.4%
1740
85.5%
207
10.2 %
183
9.0 %
4
0.2 %
Wales - Low TB
Parallel New Infection
8
724
2.5%
27
3.7%
624
86.2%
83
11.5 %
73
10.1 %
0
Parallel - Other
1
145
0.5%
4
2.8%
135
93.1%
6
4.1 %
5
3.4 %
1
0.7 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales - Low TB
10
870
3.0%
32
3.7%
759
87.2%
89
10.2 %
78
9.0 %
1
0.1 %
Scotland - Scotland
Parallel New Infection
3
401
1.4%
34
8.5%
323
80.5%
39
9.7 %
37
9.2 %
7
1.7 %
Scotland - Scotland
3
401
1.4%
34
8.5%
323
80.5%
39
9.7 %
37
9.2 %
7
1.7 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
11
515
0.9%
47
9.1%
452
87.8%
22
4.3 %
15
2.9 %
1
0.2 %
Parallel - Herd or Group Slaughter
3
1000
1.8%
44
4.4%
915
91.5%
42
4.2 %
36
3.6 %
5
0.5 %
Parallel New Infection
234
22501
39.7%
1022
4.5%
19789
87.9%
1874
8.3 %
1632
7.3 %
58
0.3 %
Parallel - new infection cull area
215
24874
43.9%
1555
6.3%
21617
86.9%
1771
7.1 %
1583
6.4 %
119
0.5 %
Parallel - Other
47
2269
4.0%
122
5.4%
1989
87.7%
150
6.6 %
147
6.5 %
11
0.5 %
Parallel Persistent Infection
92
3637
6.4%
303
8.3%
3044
83.7%
298
8.2 %
258
7.1 %
32
0.9 %
Parallel - persistent infection cull area
9
900
1.6%
36
4.0%
790
87.8%
77
8.6 %
72
8.0 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
86
286
0.5%
70
24.5%
210
73.4%
8
2.8 %
6
2.1 %
0
PAR-IR-EX
5
50
0.1%
15
30.0%
32
64.0%
4
8.0 %
3
6.0 %
0
PAR-IR-SEV
20
191
0.3%
51
26.7%
133
69.6%
9
4.7 %
7
3.7 %
0
PAR-RECUR
4
424
0.7%
25
5.9%
381
89.9%
21
5.0 %
17
4.0 %
1
0.2 %
Serial - Other
1
52
0.1%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Total
727
56699
100.0%
3290
5.8%
49403
87.1%
4277
7.5 %
3777
6.7 %
229
0.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Parallel New Infection
10
1152
2.0%
92
8.0%
1002
87.0%
59
5.1 %
55
4.8 %
3
0.3 %
Parallel - new infection cull area
202
23412
41.3%
1486
6.3%
20303
86.7%
xx-xxxx-xxxx
7.2 %
1507
6.4 %
116
0.5 %
Parallel - Other
3
468
0.8%
22
4.7%
390
83.3%
35
7.5 %
56
12.0 %
0
Parallel Persistent Infection
9
852
1.5%
141
16.5%
610
71.6%
102
12.0 %
92
10.8 %
9
1.1 %
Parallel - persistent infection cull area
9
900
1.6%
36
4.0%
790
87.8%
77
8.6 %
72
8.0 %
2
0.2 %
Serial - Other
1
52
0.1%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
129
13794
24.3%
589
4.3%
12173
88.2%
1150
8.3 %
999
7.2 %
33
0.2 %
Parallel - new infection cull area
12
1444
2.5%
69
4.8%
1308
90.6%
72
5.0 %
66
4.6 %
1
0.1 %
Parallel Persistent Infection
5
338
0.6%
10
3.0%
305
90.2%
25
7.4 %
22
6.5 %
1
0.3 %
England - LRA
Parallel New Infection
33
2911
5.1%
114
3.9%
2627
90.2%
197
6.8 %
166
5.7 %
4
0.1 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
414
45341
80.0%
2559
5.6%
39565
87.3%
3417
7.5 %
3046
6.7 %
171
0.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
7
324
0.6%
32
9.9%
280
86.4%
16
4.9 %
12
3.7 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - Herd or Group Slaughter
2
992
1.7%
43
4.3%
909
91.6%
41
4.1 %
35
3.5 %
5
0.5 %
Parallel New Infection
2
245
0.4%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
39
1551
2.7%
90
5.8%
1372
88.5%
99
6.4 %
80
5.2 %
9
0.6 %
Parallel Persistent Infection
71
2097
3.7%
134
6.4%
1819
86.7%
145
6.9 %
122
5.8 %
22
1.0 %
Parallel - Rapid Testing Two-times IRs
82
263
0.5%
64
24.3%
194
73.8%
7
2.7 %
5
1.9 %
0
PAR-IR-EX
5
50
0.1%
15
30.0%
32
64.0%
4
8.0 %
3
6.0 %
0
PAR-IR-SEV
19
190
0.3%
51
26.8%
132
69.5%
9
4.7 %
7
3.7 %
0
PAR-RECUR
3
335
0.6%
19
5.7%
302
90.1%
15
4.5 %
14
4.2 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
191
0.3%
15
7.9%
172
90.1%
6
3.1 %
3
1.6 %
1
0.5 %
Parallel - Herd or Group Slaughter
1
8
0.0%
1
12.5%
6
75.0%
1
12.5 %
1
12.5 %
0
Parallel New Infection
31
2254
4.0%
90
4.0%
1954
86.7%
224
9.9 %
203
9.0 %
7
0.3 %
Parallel - Other
3
104
0.2%
6
5.8%
91
87.5%
10
9.6 %
6
5.8 %
1
1.0 %
Parallel Persistent Infection
7
350
0.6%
18
5.1%
310
88.6%
26
7.4 %
22
6.3 %
0
Parallel - Rapid Testing Two-times IRs
3
22
0.0%
5
22.7%
16
72.7%
1
4.5 %
1
4.5 %
0
PAR-IR-SEV
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
PAR-RECUR
1
89
0.2%
6
6.7%
79
88.8%
6
6.7 %
3
3.4 %
1
1.1 %
Wales - Low TB
Parallel New Infection
24
1724
3.0%
96
5.6%
1475
85.6%
183
10.6 %
150
8.7 %
3
0.2 %
Parallel - Other
2
146
0.3%
4
2.7%
136
93.2%
6
4.1 %
5
3.4 %
1
0.7 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales
308
10937
19.3%
696
6.4%
9498
86.8%
819
7.5 %
692
6.3 %
51
0.5 %
Scotland - Scotland
Parallel New Infection
5
421
0.7%
35
8.3%
340
80.8%
41
9.7 %
39
9.3 %
7
1.7 %
Scotland
5
421
0.7%
35
8.3%
340
80.8%
41
9.7 %
39
9.3 %
7
1.7 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
0
70
0
1
August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
0
65
0
1
October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
0
1
7
3
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
0
0
9
1
1005
8
65
1
0
2021 January
375
27940
233
1653
2
123
0
0
0
0
102
0
11
6
0
1321
86
0
0
1
February
352
28759
235
1637
540
781
0
0
0
0
9
0
0
0
0
106
8
0
0
0
Totals (last
12 months)
3573
276726
2090
12768
547
1048
0
0
0
0
1124
15
12
54
4
9025
103
632
1
4
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1879
215362
1226
9433
892
0
0
0
0
1011
7
7
21
3
6909
20
42
0
2
Scotland
57
5268
31
172
19
0
0
0
0
15
0
0
4
1
78
3
51
0
0
Wales
1637
56096
833
3163
137
0
0
0
0
98
8
5
29
0
2038
80
539
1
2
Totals
3573
276726
2090
12768
1048
0
0
0
0
1124
15
12
54
4
9025
103
632
1
4
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25839
1653
26496
1637
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
Worcs
Warks
S Wales
Somerset excl N
S Glamorgan
Pembs
N Yorks
NE Wales
Leics & Rut
Heref
Gwynedd
Gr Manchester
Dorset
Derbyshire
Corn & Scilly
Ceredigion
Bucks
Ayrshire
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during February 2021 (% submission per
County)
County
%Resampled %Retested
Avon
29.51 %
29.51 %
Ayrshire
9.23 %
9.73 %
Berks
5.91 %
7.17 %
Bucks
Carms
4.59 %
6.97 %
Ceredigion
5.38 %
8.06 %
Cheshire
4.20 %
4.90 %
Corn & Scilly
13.31 %
14.52 %
Cumbria
55.56 %
55.56 %
Derbyshire
9.11 %
10.21 %
Devon
5.65 %
6.12 %
Dorset
5.30 %
6.09 %
Gloucs
10.58 %
11.24 %
Gr Manchester
Gwent
9.09 %
Gwynedd
10.53 %
10.53 %
Hampshire
2.23 %
2.46 %
Heref
8.65 %
8.65 %
Herts
Leics & Rut
7.01 %
8.03 %
Lincs
6.59 %
6.59 %
NE Wales
8.93 %
9.84 %
Northants
8.55 %
9.44 %
N Yorks
4.19 %
4.79 %
Oxon
8.25 %
8.64 %
Pembs
4.18 %
4.69 %
Powys
7.80 %
9.19 %
S Glamorgan
Shropshire
6.11 %
6.59 %
Somerset excl
4.03 %
5.79 %
Staffs
8.19 %
8.85 %
S Wales
3.53 %
4.13 %
S Yorks
15.34 %
15.34 %
Warks
5.38 %
5.67 %
Wiltshire
10.53 %
10.53 %

Worcs

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
21756
1205
5.5%
22216
1205
5.4%
Wales
6008
398
6.6%
6142
398
6.5%
Scotland
369
34
9.2%
401
34
8.5%
GB
28133
1637
5.8%
28759
1637
5.7%
Table 6a (i): Number of animals and submissions tested in February 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
43839
2559
5.8%
45341
2559
5.6%
Wales
10526
696
6.6%
10937
696
6.4%
Scotland
389
35
9.0%
421
35
8.3%
GB
54752
3290
6.0%
56699
3290
5.8%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
26941
973
3.6%
27498
973
3.5%
Wales
5430
315
5.8%
5486
315
5.7%
Scotland
162
1
0.6%
162
1
0.6%
GB
32533
1289
4.0%
33146
1289
3.9%
Table 6a (ii): Number of animals and submissions tested in February 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
49708
1982
4.0%
51745
1982
3.8%
Wales
10872
650
6.0%
11243
650
5.8%
Scotland
1387
24
1.7%
1432
24
1.7%
GB
61967
2656
4.3%
64420
2656
4.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Wiltshire
PRV-SE
1
10
0
0
Total
3
15
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples February 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Wiltshire
PRV-SE
1
10
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
6
20
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
England
1
90
0
0
Wales
Carmarthenshire
BOV-IR-C
3
35
10
28.6%
BOV-IR-EX
1
3
2
66.7%
OTHER
1
23
6
26.1%
Ceredigion
BOV-IR-C
2
14
2
14.3%
BOV-OTHER
3
23
9
39.1%
Pembrokeshire
1
16
1
6.3%
BOV-IR-C
5
88
15
17.0%
BOV-IR-EX
1
3
1
33.3%
BOV-OTHER
4
218
19
8.7%
BOV-P
1
21
0
0
Powys
BOV-IR-C
2
7
2
28.6%
BOV-IR-EX
1
32
7
21.9%
BOV-OTHER
2
107
18
16.8%
Wales
27
590
92
15.6%
All GB
28
680
92
13.5 %
Table 8a Number of Cattle Antibody Tests February 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
Devon
1
95
6
6.3%
England
2
185
6
3.2%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
3
35
10
28.6%
BOV-IR-EX
1
3
2
66.7%
OTHER
1
23
6
26.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
2
14
2
14.3%
BOV-OTHER
3
23
9
39.1%
North East Wales
2
24
6
25.0%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
5
88
15
17.0%
BOV-IR-EX
1
3
1
33.3%
BOV-OTHER
4
218
19
8.7%
BOV-P
1
21
0
0
Powys
2
11
1
9.1%
BOV-IR-C
2
7
2
28.6%
BOV-IR-EX
1
32
7
21.9%
BOV-OTHER
2
107
18
16.8%
South Wales
4
14
5
35.7%
Wales
58
862
137
15.9%
All GB
60
1,047
143
13.7 %

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive