Monthly Report: Surveillance Project SB4008 IFNg (TC0651 and TC0751) & Antibody (TC0077) tests for bovine tuberculosis-March 2024
Source: environment.data.gov.uk (2025-04-29)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2024. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.
Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).
Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period
Issued 09 April 2024
211
1st March - 31st March 2024

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
March 2024
%
Total 2024
%
Submissions
215
706
Samples
Total (%)
19876
100.00 %
62381
100.00 %
England
13073
65.77 %
40376
64.72 %
Wales
6373
32.06 %
21167
33.93 %
Scotland
430
2.16 %
838
1.34 %
Parallel Tests
PAR-CHR
0
0.00 %
411
0.66 %
PAR-CUL-N
1
0.01 %
381
0.61 %
PAR-CUL-P
1041
5.24 %
2425
3.89 %
PAR-HERD-S
0
0.00 %
44
0.07 %
PAR-IR-EX
14
0.07 %
112
0.18 %
PAR-IR-SEV
208
1.05 %
892
1.43 %
PAR-NEW-IN
3660
18.41 %
11562
18.53 %
PAR-OTHER
929
4.67 %
1837
2.94 %
PAR-PERSIS
4583
23.06 %
12769
20.47 %
PAR-POSTM
0
0.00 %
PAR-RAPID
36
0.18 %
193
0.31 %
PAR-RECUR
9334
46.96 %
29567
47.40 %
Total(% of all samples)
19806
99.65 %
60193
96.49 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
70
0.35 %
1920
3.08 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
268
0.43 %
Total(% of all samples)
70
0.35 %
2188
3.51 %
Total
19876
100.00 %
62381
100.00 %
Retests
Total (% of all samples)
1419
7.14 %
5029
8.06 %
England
1019
5.13 %
3463
5.55 %
Wales
359
1.81 %
1470
2.36 %
Scotland
41
0.21 %
96
0.15 %
Resamples
Total (% of all samples)
1194
6.01 %
4381
7.02 %
England
883
4.44 %
3067
4.92 %
Wales
280
1.41 %
1232
1.97 %
Scotland
31
0.16 %
82
0.13 %
Rejects
Total (% of all samples)
186
0.94 %
1022
1.64 %
England
66
0.33 %
869
1.39 %
Wales
120
0.60 %
152
0.24 %
Scotland
0
0.00 %
1
0.00 %
Table 1. Monthly Totals for March 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
1
0.0 %
PAR-CUL-P
1041
5.2 %
PAR-IR-EX
14
0.1 %
PAR-IR-SEV
208
1.0 %
PAR-NEW-IN
3660
18.4 %
PAR-OTHER
929
4.7 %
PAR-PERSIS
4583
23.1 %
PAR-RAPID
36
0.2 %
PAR-RECUR
9334
47.0 %
SER-FLEXI
70
0.4 %
Total
19876
100.00 %
Figure 1. Number of samples submitted in each category during March 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
3500
4000
Wigtown
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Notts
Northants
Leics & Rut
Humberside
Heref
Gloucs
E Sussex
Dorset
Devon
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during March 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
Wigtown
West lothian
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
Humberside
Herts
Heref
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
41
0.21 %
1
2.44 %
39
95.12 %
1
2.44 %
1
2.44 %
0
0.00 %
PAR-RECUR
3
330
1.66 %
14
4.24 %
294
89.09 %
27
8.18 %
22
6.67 %
0
0.00 %
Corn & Scilly
PAR-OTHER
1
337
1.70 %
28
8.31 %
280
83.09 %
37
10.98 %
29
8.61 %
0
0.00 %
PAR-PERSIS
6
582
2.93 %
66
11.34 %
474
81.44 %
49
8.42 %
42
7.22 %
0
0.00 %
PAR-RECUR
10
405
2.04 %
34
8.40 %
314
77.53 %
65
16.05 %
57
14.07 %
0
0.00 %
SER-FLEXI
2
10
0.05 %
1
10.00 %
8
80.00 %
0
0.00 %
0
0.00 %
1
10.00 %
Devon
PAR-CUL-N
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
162
0.82 %
5
3.09 %
135
83.33 %
22
13.58 %
22
13.58 %
0
0.00 %
PAR-PERSIS
2
221
1.11 %
15
6.79 %
191
86.43 %
15
6.79 %
14
6.33 %
1
0.45 %
PAR-RECUR
4
1,149
5.78 %
54
4.70 %
1001
87.12 %
89
7.75 %
72
6.27 %
22
1.91 %
Dorset
PAR-RECUR
1
37
0.19 %
3
8.11 %
28
75.68 %
7
18.92 %
6
16.22 %
0
0.00 %
Gloucs
PAR-RECUR
6
887
4.46 %
77
8.68 %
783
88.28 %
27
3.04 %
27
3.04 %
0
0.00 %
Heref
PAR-OTHER
1
150
0.75 %
10
6.67 %
131
87.33 %
11
7.33 %
9
6.00 %
0
0.00 %
PAR-RECUR
3
741
3.73 %
14
1.89 %
653
88.12 %
87
11.74 %
74
9.99 %
0
0.00 %
Oxon
PAR-RECUR
3
1,225
6.16 %
43
3.51 %
1162
94.86 %
29
2.37 %
20
1.63 %
0
0.00 %
Shropshire
PAR-CUL-P
2
357
1.80 %
39
10.92 %
308
86.27 %
10
2.80 %
10
2.80 %
0
0.00 %
PAR-RECUR
4
989
4.98 %
45
4.55 %
860
86.96 %
96
9.71 %
82
8.29 %
2
0.20 %
Somerset excl N
PAR-CUL-P
2
684
3.44 %
20
2.92 %
617
90.20 %
42
6.14 %
36
5.26 %
11
1.61 %
PAR-RECUR
5
642
3.23 %
58
9.03 %
552
85.98 %
35
5.45 %
31
4.83 %
1
0.16 %
Staffs
PAR-PERSIS
1
14
0.07 %
5
35.71 %
7
50.00 %
3
21.43 %
2
14.29 %
0
0.00 %
PAR-RECUR
6
143
0.72 %
4
2.80 %
132
92.31 %
13
9.09 %
7
4.90 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
1
3
0.02 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
Bucks
PAR-NEW-IN
1
3
0.02 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Cheshire
PAR-OTHER
1
268
1.35 %
11
4.10 %
253
94.40 %
5
1.87 %
4
1.49 %
0
0.00 %
PAR-PERSIS
6
589
2.96 %
64
10.87 %
409
69.44 %
126
21.39 %
116
19.69 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
67
0.34 %
2
2.99 %
63
94.03 %
3
4.48 %
2
2.99 %
0
0.00 %
Table 2a. Summary by County for March 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
6
685
3.45 %
16
2.34 %
624
91.09 %
48
7.01 %
44
6.42 %
1
0.15 %
Northants
PAR-NEW-IN
4
338
1.70 %
21
6.21 %
281
83.14 %
15
4.44 %
14
4.14 %
22
6.51 %
Notts
PAR-NEW-IN
3
376
1.89 %
10
2.66 %
315
83.78 %
53
14.10 %
51
13.56 %
0
0.00 %
Oxon
PAR-NEW-IN
1
113
0.57 %
8
7.08 %
97
85.84 %
8
7.08 %
8
7.08 %
0
0.00 %
PAR-RECUR
4
981
4.94 %
34
3.47 %
908
92.56 %
45
4.59 %
35
3.57 %
4
0.41 %
Warks
PAR-RECUR
1
37
0.19 %
1
2.70 %
26
70.27 %
10
27.03 %
10
27.03 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
3
429
2.16 %
16
3.73 %
386
89.98 %
29
6.76 %
26
6.06 %
1
0.23 %
Humberside
PAR-NEW-IN
1
77
0.39 %
0
0.00 %
71
92.21 %
8
10.39 %
6
7.79 %
0
0.00 %
England
99
13073
65.77 %
719
5.50 %
11405
87.24 %
1019
7.79 %
883
6.75 %
66
0.50 %
Wales - High TB
Carms
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
44
0.22 %
20
45.45 %
22
50.00 %
2
4.55 %
2
4.55 %
0
0.00 %
PAR-OTHER
1
7
0.04 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-PERSIS
2
407
2.05 %
6
1.47 %
391
96.07 %
11
2.70 %
9
2.21 %
1
0.25 %
PAR-RAPID
4
14
0.07 %
7
50.00 %
6
42.86 %
2
14.29 %
1
7.14 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
8
0.04 %
5
62.50 %
3
37.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
17
0.09 %
9
52.94 %
8
47.06 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
75
0.38 %
3
4.00 %
71
94.67 %
2
2.67 %
1
1.33 %
0
0.00 %
Pembs
PAR-IR-EX
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-IR-SEV
15
110
0.55 %
42
38.18 %
57
51.82 %
13
11.82 %
11
10.00 %
0
0.00 %
PAR-OTHER
1
5
0.03 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-PERSIS
14
2,528
12.72 %
101
4.00 %
2266
89.64 %
78
3.09 %
50
1.98 %
111
4.39 %
PAR-RAPID
5
12
0.06 %
3
25.00 %
8
66.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-RECUR
10
1,119
5.63 %
38
3.40 %
1032
92.23 %
61
5.45 %
45
4.02 %
4
0.36 %
SER-FLEXI
2
15
0.08 %
0
0.00 %
13
86.67 %
3
20.00 %
2
13.33 %
0
0.00 %
Powys
PAR-IR-SEV
3
21
0.11 %
4
19.05 %
15
71.43 %
2
9.52 %
2
9.52 %
0
0.00 %
PAR-RAPID
2
5
0.03 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
7
0.04 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
Wales - Intermediate TB
NE Wales
PAR-IR-EX
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
7
0.04 %
4
57.14 %
3
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
7
404
2.03 %
14
3.47 %
349
86.39 %
44
10.89 %
38
9.41 %
3
0.74 %
PAR-PERSIS
3
143
0.72 %
5
3.50 %
134
93.71 %
4
2.80 %
4
2.80 %
0
0.00 %
PAR-RECUR
3
378
1.90 %
3
0.79 %
350
92.59 %
26
6.88 %
25
6.61 %
0
0.00 %
Powys
PAR-NEW-IN
2
67
0.34 %
4
5.97 %
53
79.10 %
11
xx-xxxx-xxxx %
10
14.93 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
8
295
1.48 %
16
5.42 %
260
88.14 %
24
8.14 %
19
6.44 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RECUR
1
18
0.09 %
0
0.00 %
18
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
5
0.03 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
1
5
0.03 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
2
373
1.88 %
20
5.36 %
324
86.86 %
43
11.53 %
29
7.77 %
0
0.00 %
PAR-PERSIS
1
58
0.29 %
5
8.62 %
53
91.38 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
171
0.86 %
3
1.75 %
148
86.55 %
19
11.11 %
19
11.11 %
1
0.58 %
SER-FLEXI
1
40
0.20 %
9
22.50 %
28
70.00 %
4
10.00 %
3
7.50 %
0
0.00 %
Wales
114
6373
32.06 %
328
5.15 %
5645
88.58 %
359
5.63 %
280
4.39 %
120
1.88 %
Scotland - Scotland
Wigtown
PAR-NEW-IN
2
430
2.16 %
7
1.63 %
392
91.16 %
41
9.53 %
31
7.21 %
0
0.00 %
Scotland
2
430
2.16 %
7
1.63 %
392
91.16 %
41
9.53 %
31
7.21 %
0
0.00 %
Grand Total
Sum:
215
19876
1054
17442
1419
1194
186
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
3
333
0.53 %
47
14.11 %
264
79.28 %
24
7.21 %
22
6.61 %
0
0.00 %
PAR-PERSIS
5
756
1.21 %
91
12.04 %
621
82.14 %
48
6.35 %
44
5.82 %
0
0.00 %
PAR-RECUR
7
730
1.17 %
35
4.79 %
638
87.40 %
68
9.32 %
57
7.81 %
0
0.00 %
Corn & Scilly
PAR-CUL-P
2
891
1.43 %
35
3.93 %
805
90.35 %
58
6.51 %
51
5.72 %
0
0.00 %
PAR-OTHER
3
947
1.52 %
98
10.35 %
766
80.89 %
97
10.24 %
83
8.76 %
0
0.00 %
PAR-PERSIS
11
1,491
2.39 %
124
8.32 %
1218
81.69 %
169
11.33 %
148
9.93 %
1
0.07 %
PAR-RECUR
28
2,236
3.58 %
179
8.01 %
1700
76.03 %
382
17.08 %
353
15.79 %
4
0.18 %
SER-FLEXI
4
45
0.07 %
12
26.67 %
30
66.67 %
2
4.44 %
2
4.44 %
1
2.22 %
Devon
PAR-CUL-N
4
239
0.38 %
13
5.44 %
212
88.70 %
17
7.11 %
14
5.86 %
0
0.00 %
PAR-CUL-P
2
8
0.01 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-OTHER
1
162
0.26 %
5
3.09 %
135
83.33 %
22
13.58 %
22
13.58 %
0
0.00 %
PAR-PERSIS
8
1,066
1.71 %
64
6.00 %
912
85.55 %
97
9.10 %
87
8.16 %
3
0.28 %
PAR-RECUR
19
2,551
4.09 %
119
4.66 %
2251
88.24 %
184
7.21 %
157
6.15 %
24
0.94 %
Dorset
PAR-RECUR
6
1,337
2.14 %
97
7.26 %
1183
88.48 %
61
4.56 %
55
4.11 %
2
0.15 %
SER-FLEXI
3
53
0.08 %
10
18.87 %
42
79.25 %
1
1.89 %
1
1.89 %
0
0.00 %
Gloucs
PAR-RECUR
14
2,190
3.51 %
124
5.66 %
1735
79.22 %
77
3.52 %
72
3.29 %
259
11.83 %
Heref
PAR-CUL-N
1
142
0.23 %
13
9.15 %
119
83.80 %
12
8.45 %
10
7.04 %
0
0.00 %
PAR-CUL-P
1
485
0.78 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
485
100.00 %
PAR-OTHER
1
150
0.24 %
10
6.67 %
131
87.33 %
11
7.33 %
9
6.00 %
0
0.00 %
PAR-PERSIS
1
80
0.13 %
1
1.25 %
77
96.25 %
2
2.50 %
2
2.50 %
0
0.00 %
PAR-RECUR
13
2,745
4.40 %
131
4.77 %
2398
87.36 %
247
9.00 %
215
7.83 %
1
0.04 %
Oxon
PAR-RECUR
3
1,225
1.96 %
43
3.51 %
1162
94.86 %
29
2.37 %
20
1.63 %
0
0.00 %
Shropshire
PAR-CUL-P
2
357
0.57 %
39
10.92 %
308
86.27 %
10
2.80 %
10
2.80 %
0
0.00 %
PAR-RECUR
17
2,709
4.34 %
153
5.65 %
2351
86.78 %
221
8.16 %
192
7.09 %
13
0.48 %
SER-FLEXI
2
67
0.11 %
13
19.40 %
41
61.19 %
18
26.87 %
13
19.40 %
0
0.00 %
Somerset excl N
PAR-CHR
2
78
0.13 %
4
5.13 %
73
93.59 %
2
2.56 %
1
1.28 %
0
0.00 %
Table 2b. Summary by County for 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
2
684
1.10 %
20
2.92 %
617
90.20 %
42
6.14 %
36
5.26 %
11
1.61 %
PAR-RECUR
11
1,304
2.09 %
83
6.37 %
1155
88.57 %
70
5.37 %
63
4.83 %
3
0.23 %
Staffs
PAR-PERSIS
2
233
0.37 %
53
22.75 %
162
69.53 %
27
11.59 %
18
7.73 %
0
0.00 %
PAR-RECUR
14
1,235
1.98 %
42
3.40 %
1094
88.58 %
107
8.66 %
95
7.69 %
4
0.32 %
Wiltshire
PAR-RECUR
10
1,616
2.59 %
58
3.59 %
1489
92.14 %
79
4.89 %
69
4.27 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
4
386
0.62 %
25
6.48 %
345
89.38 %
17
4.40 %
13
3.37 %
3
0.78 %
Bucks
PAR-NEW-IN
3
40
0.06 %
2
5.00 %
34
85.00 %
4
10.00 %
4
10.00 %
0
0.00 %
PAR-RECUR
3
220
0.35 %
16
7.27 %
188
85.45 %
18
8.18 %
16
7.27 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
1,061
1.70 %
32
3.02 %
792
74.65 %
287
27.05 %
237
22.34 %
0
0.00 %
PAR-OTHER
1
268
0.43 %
11
4.10 %
253
94.40 %
5
1.87 %
4
1.49 %
0
0.00 %
PAR-PERSIS
10
1,638
2.63 %
103
6.29 %
1360
83.03 %
198
12.09 %
175
10.68 %
0
0.00 %
Derbyshire
PAR-RECUR
6
668
1.07 %
17
2.54 %
610
91.32 %
45
6.74 %
39
5.84 %
2
0.30 %
E Sussex
PAR-NEW-IN
7
659
1.06 %
35
5.31 %
589
89.38 %
38
5.77 %
34
5.16 %
1
0.15 %
Leics & Rut
PAR-NEW-IN
13
1,749
2.80 %
48
2.74 %
1532
87.59 %
189
10.81 %
168
9.61 %
1
0.06 %
Northants
PAR-NEW-IN
11
753
1.21 %
42
5.58 %
619
82.20 %
74
9.83 %
68
9.03 %
24
3.19 %
Notts
PAR-NEW-IN
5
417
0.67 %
11
2.64 %
353
84.65 %
56
13.43 %
53
12.71 %
0
0.00 %
Oxon
PAR-HERD-S
1
9
0.01 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-NEW-IN
2
361
0.58 %
22
6.09 %
302
83.66 %
39
10.80 %
37
10.25 %
0
0.00 %
PAR-RECUR
6
1,265
2.03 %
42
3.32 %
1156
91.38 %
74
5.85 %
63
4.98 %
4
0.32 %
Warks
PAR-RECUR
5
627
1.01 %
26
4.15 %
513
81.82 %
73
11.64 %
88
14.04 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
2
33
0.05 %
2
6.06 %
9
27.27 %
5
15.15 %
5
15.15 %
17
51.52 %
Cumbria
PAR-NEW-IN
5
611
0.98 %
18
2.95 %
551
90.18 %
44
7.20 %
41
6.71 %
1
0.16 %
SER-OTHER
1
7
0.01 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Herts
PAR-NEW-IN
2
203
0.33 %
10
4.93 %
180
88.67 %
13
6.40 %
13
6.40 %
0
0.00 %
Humberside
PAR-NEW-IN
1
77
0.12 %
0
0.00 %
71
92.21 %
8
10.39 %
6
7.79 %
0
0.00 %
Kent
SER-OTHER
2
248
0.40 %
1
0.40 %
240
96.77 %
9
3.63 %
6
2.42 %
1
0.40 %
Lancashire
PAR-NEW-IN
2
103
0.17 %
1
0.97 %
98
95.15 %
5
4.85 %
3
2.91 %
1
0.97 %
Lincs
PAR-NEW-IN
2
221
0.35 %
3
1.36 %
207
93.67 %
12
5.43 %
11
4.98 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Norfolk
PAR-NEW-IN
2
87
0.14 %
7
8.05 %
54
62.07 %
24
27.59 %
24
27.59 %
2
2.30 %
N Yorks
PAR-NEW-IN
4
507
0.81 %
7
1.38 %
465
91.72 %
38
7.50 %
34
6.71 %
1
0.20 %
W Sussex
SER-OTHER
2
13
0.02 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
306
40376
64.72 %
2197
5.44 %
34243
84.81 %
3463
8.58 %
3067
7.60 %
869
2.15 %
Wales - High TB
Carms
PAR-IR-EX
5
16
0.03 %
4
25.00 %
9
56.25 %
3
18.75 %
3
18.75 %
0
0.00 %
PAR-IR-SEV
20
149
0.24 %
66
44.30 %
75
50.34 %
8
5.37 %
6
4.03 %
2
1.34 %
PAR-OTHER
4
230
0.37 %
19
8.26 %
197
85.65 %
20
8.70 %
14
6.09 %
0
0.00 %
PAR-PERSIS
8
964
1.55 %
76
7.88 %
841
87.24 %
54
5.60 %
46
4.77 %
1
0.10 %
PAR-RAPID
15
57
0.09 %
26
45.61 %
29
50.88 %
3
5.26 %
2
3.51 %
0
0.00 %
PAR-RECUR
6
2,576
4.13 %
46
1.79 %
2478
96.20 %
73
2.83 %
51
1.98 %
1
0.04 %
Ceredigion
PAR-HERD-S
1
35
0.06 %
6
17.14 %
27
77.14 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-IR-EX
4
15
0.02 %
11
73.33 %
4
26.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
28
0.04 %
12
42.86 %
16
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
7
0.01 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
2
17
0.03 %
8
47.06 %
7
41.18 %
2
11.76 %
2
11.76 %
0
0.00 %
PAR-IR-SEV
3
8
0.01 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
72
0.12 %
8
11.11 %
63
87.50 %
1
1.39 %
1
1.39 %
0
0.00 %
PAR-RAPID
5
17
0.03 %
11
64.71 %
6
35.29 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
164
0.26 %
4
2.44 %
149
90.85 %
13
7.93 %
11
6.71 %
0
0.00 %
Pembs
PAR-IR-EX
9
18
0.03 %
10
55.56 %
7
38.89 %
1
5.56 %
1
5.56 %
0
0.00 %
PAR-IR-SEV
56
573
0.92 %
211
36.82 %
322
56.20 %
45
7.85 %
40
6.98 %
0
0.00 %
PAR-OTHER
3
7
0.01 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-PERSIS
32
4,873
7.81 %
290
5.95 %
4270
87.63 %
261
5.36 %
199
4.08 %
114
2.34 %
PAR-RAPID
21
60
0.10 %
17
28.33 %
42
70.00 %
1
1.67 %
1
1.67 %
0
0.00 %
PAR-RECUR
22
2,306
3.70 %
93
4.03 %
2098
90.98 %
142
6.16 %
107
4.64 %
8
0.35 %
SER-FLEXI
11
1,313
2.10 %
75
5.71 %
1133
86.29 %
118
8.99 %
103
7.84 %
2
0.15 %
Powys
PAR-IR-EX
2
16
0.03 %
4
25.00 %
11
68.75 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
7
60
0.10 %
28
46.67 %
30
50.00 %
2
3.33 %
2
3.33 %
0
0.00 %
PAR-OTHER
2
73
0.12 %
2
2.74 %
66
90.41 %
7
9.59 %
4
5.48 %
1
1.37 %
PAR-PERSIS
2
206
0.33 %
10
4.85 %
159
77.18 %
37
17.96 %
37
17.96 %
0
0.00 %
PAR-RAPID
3
6
0.01 %
4
66.67 %
2
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
14
1,037
1.66 %
60
5.79 %
931
89.78 %
56
5.40 %
44
4.24 %
2
0.19 %
SER-FLEXI
2
177
0.28 %
26
14.69 %
98
55.37 %
60
33.90 %
53
29.94 %
0
0.00 %
S Wales
PAR-RECUR
2
28
0.04 %
4
14.29 %
22
78.57 %
1
3.57 %
1
3.57 %
1
3.57 %
Wales - Intermediate TB
Carms
PAR-PERSIS
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
21
0.03 %
17
80.95 %
4
19.05 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
5
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
9
36
0.06 %
11
30.56 %
21
58.33 %
4
11.11 %
4
11.11 %
0
0.00 %
PAR-NEW-IN
19
1,684
2.70 %
60
3.56 %
1484
88.12 %
151
8.97 %
130
7.72 %
10
0.59 %
PAR-PERSIS
18
1,327
2.13 %
65
4.90 %
1207
90.96 %
54
4.07 %
49
3.69 %
6
0.45 %
PAR-RAPID
3
5
0.01 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
588
0.94 %
18
3.06 %
527
89.63 %
45
7.65 %
43
7.31 %
0
0.00 %
SER-FLEXI
2
128
0.21 %
21
xx-xxxx-xxxx %
100
78.13 %
8
6.25 %
7
5.47 %
0
0.00 %
Powys
PAR-IR-SEV
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
113
0.18 %
5
4.42 %
95
84.07 %
14
12.39 %
13
11.50 %
0
0.00 %
PAR-RAPID
2
18
0.03 %
4
22.22 %
11
61.11 %
3
16.67 %
3
16.67 %
0
0.00 %
PAR-RECUR
2
21
0.03 %
1
4.76 %
17
80.95 %
4
19.05 %
3
14.29 %
0
0.00 %
S Glamorgan
PAR-RAPID
2
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
3
12
0.02 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-NEW-IN
15
691
1.11 %
24
3.47 %
593
85.82 %
80
11.58 %
71
10.27 %
3
0.43 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RECUR
1
18
0.03 %
0
0.00 %
18
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
10
0.02 %
3
30.00 %
6
60.00 %
1
10.00 %
1
10.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
NE Wales
PAR-IR-SEV
3
22
0.04 %
8
36.36 %
13
59.09 %
1
4.55 %
1
4.55 %
0
0.00 %
PAR-NEW-IN
7
968
1.55 %
42
4.34 %
795
82.13 %
148
15.29 %
131
13.53 %
0
0.00 %
PAR-PERSIS
1
58
0.09 %
5
8.62 %
53
91.38 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
9
0.01 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RECUR
1
171
0.27 %
3
1.75 %
148
86.55 %
19
11.11 %
19
11.11 %
1
0.58 %
SER-FLEXI
2
127
0.20 %
26
20.47 %
82
64.57 %
20
15.75 %
19
14.96 %
0
0.00 %
Wales
394
21167
33.93 %
1465
6.92 %
18318
86.54 %
1470
6.94 %
1232
5.82 %
152
0.72 %
Scotland - Scotland
West lothian
PAR-NEW-IN
1
27
0.04 %
0
0.00 %
16
59.26 %
11
40.74 %
11
40.74 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
811
1.30 %
19
2.34 %
720
88.78 %
85
10.48 %
71
8.75 %
1
0.12 %
Scotland
6
838
1.34 %
19
2.27 %
736
87.83 %
96
11.46 %
82
9.79 %
1
0.12 %
Grand Total
Sum:
706
62381
3681
53297
5029
4381
1022
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol March 2024
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
97
13063
99.9%
718
5.5%
11397
87.2%
1019
7.8%
883
6.8%
65
0.5%
Serial/Extended
2
10
0.1%
1
10.0%
8
80.0%
0
0.0 %
0
0.0 %
1
10.0%
Total
99
13073
100.0%
719
5.5%
11405
87.2%
1019
7.8%
883
6.8%
66
0.5%
Scotland
Parallel
2
430
100.0%
7
1.6%
392
91.2%
41
9.5%
31
7.2%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
430
100.0%
7
1.6%
392
91.2%
41
9.5%
31
7.2%
0
0.0 %
Wales
Parallel
109
6313
99.1%
318
5.0%
5601
88.7%
351
5.6%
274
4.3%
120
1.9%
Serial/Extended
5
60
0.9%
10
16.7%
44
73.3%
8
13.3%
6
10.0%
0
0.0 %
Total
114
6373
100.0%
328
5.1%
5645
88.6%
359
5.6%
280
4.4%
120
1.9%
GB
Parallel
208
19806
99.6%
1043
5.3%
17390
87.8%
1411
7.1%
1188
6.0%
185
0.9%
Serial/Extended
7
70
0.4%
11
15.7%
52
74.3%
8
11.4%
6
8.6%
1
1.4%
Total
215
19876
100.0%
1054
5.3%
17442
87.8%
1419
7.1%
1194
6.0%
186
0.9%
99.00
114.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2024)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
292
39943
98.9%
2161
5.4%
33870
84.8%
3433
8.6%
3045
7.6%
867
2.2%
Serial/Extended
14
433
1.1%
36
8.3%
373
86.1%
30
6.9%
22
5.1%
2
0.5%
Total
306
40376
100.0%
2197
5.4%
34243
84.8%
3463
8.6%
3067
7.6%
869
2.2%
Scotland
Parallel
6
838
100.0%
19
2.3%
736
87.8%
96
11.5%
82
9.8%
1
0.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
6
838
100.0%
19
2.3%
736
87.8%
96
11.5%
82
9.8%
1
0.1%
Wales
Parallel
374
19412
91.7%
1314
6.8%
xx-xxxx-xxxx
87.1%
1263
6.5%
1049
5.4%
150
0.8%
Serial/Extended
20
1755
8.3%
151
8.6%
1419
80.9%
207
11.8%
183
10.4%
2
0.1%
Total
394
21167
100.0%
1465
6.9%
18318
86.5%
1470
6.9%
1232
5.8%
152
0.7%
GB
Parallel
672
60193
96.5%
3494
5.8%
51505
85.6%
4792
8.0%
4176
6.9%
1018
1.7%
Serial/Extended
34
2188
3.5%
187
8.5%
1792
81.9%
237
10.8%
205
9.4%
4
0.2%
Total
706
62381
100.0%
3681
5.9%
53297
85.4%
5029
8.1%
4381
7.0%
1022
1.6%
306.00
394.00
6.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
7
70
0.4%
11
15.7%
52
74.3%
8
11.4 %
6
8.6 %
1
1.4 %
Parallel - IRs in Exit Strategy for persistent her
6
14
0.1%
6
42.9%
7
50.0%
1
7.1 %
1
7.1 %
0
Parallel New Infection
43
3660
18.4%
134
3.7%
3217
87.9%
331
9.0 %
282
7.7 %
27
0.7 %
Parallel - new infection cull area
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Other
6
929
4.7%
54
5.8%
808
87.0%
78
8.4 %
67
7.2 %
0
Parallel Persistent Infection
36
4583
23.1%
268
5.8%
3964
86.5%
287
6.3 %
238
5.2 %
113
2.5 %
Parallel - persistent infection cull area
4
1041
5.2%
59
5.7%
925
88.9%
52
5.0 %
46
4.4 %
11
1.1 %
Parallel - Rapid Testing Two-times IRs
15
36
0.2%
14
38.9%
18
50.0%
5
13.9 %
4
11.1 %
0
Parallel - Recurrent breakdowns
67
9334
47.0%
428
4.6%
8337
89.3%
640
6.9 %
535
5.7 %
34
0.4 %
Parallel - severe IRs - in persistent herds
30
208
1.0%
80
38.5%
113
54.3%
17
8.2 %
15
7.2 %
0
Total
215
19876
100.0%
1054
5.3%
17442
87.8%
1419
7.1 %
1194
6.0 %
186
0.9 %
Table 4a. Summaryof IFN Gamma results by submission reason (March 2024)
England - HRA
Extended Flexible IFN-GAMMA test
2
10
0.1%
1
10.0%
8
80.0%
0
0
1
10.0 %
Parallel - new infection cull area
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Other
3
649
3.3%
43
6.6%
546
84.1%
70
10.8 %
60
9.2 %
0
Parallel Persistent Infection
10
858
4.3%
87
10.1%
711
82.9%
68
7.9 %
59
6.9 %
1
0.1 %
Parallel - persistent infection cull area
4
1041
5.2%
59
5.7%
925
88.9%
52
5.0 %
46
4.4 %
11
1.1 %
Parallel - Recurrent breakdowns
45
6548
32.9%
346
5.3%
5779
88.3%
475
7.3 %
398
6.1 %
25
0.4 %
England - HRA
65
9107
45.8%
536
5.9%
7970
87.5%
665
7.3 %
563
6.2 %
38
0.4 %
England - Edge
Parallel New Infection
18
1585
8.0%
57
3.6%
1382
87.2%
131
8.3 %
123
7.8 %
23
1.5 %
Parallel - Other
1
268
1.3%
11
4.1%
253
94.4%
5
1.9 %
4
1.5 %
0
Parallel Persistent Infection
6
589
3.0%
64
10.9%
409
69.4%
126
21.4 %
116
19.7 %
0
Parallel - Recurrent breakdowns
5
1018
5.1%
35
3.4%
934
91.7%
55
5.4 %
45
4.4 %
4
0.4 %
England - Edge
30
3460
17.4%
167
4.8%
2978
86.1%
317
9.2 %
288
8.3 %
27
0.8 %
England - LRA
Parallel New Infection
4
506
2.5%
16
3.2%
457
90.3%
37
7.3 %
32
6.3 %
1
0.2 %
England - LRA
4
506
2.5%
16
3.2%
457
90.3%
37
7.3 %
32
6.3 %
1
0.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
2
15
0.1%
0
0.0 %
13
86.7%
3
20.0 %
2
13.3 %
0
Parallel - IRs in Exit Strategy for persistent her
5
13
0.1%
5
38.5%
7
53.8%
1
7.7 %
1
7.7 %
0
Parallel - Other
2
12
0.1%
0
0.0 %
9
75.0%
3
25.0 %
3
25.0 %
0
Parallel Persistent Infection
16
2935
14.8%
107
3.6%
2657
90.5%
89
3.0 %
59
2.0 %
112
3.8 %
Parallel - Rapid Testing Two-times IRs
11
31
0.2%
14
45.2%
15
48.4%
3
9.7 %
2
6.5 %
0
Parallel - Recurrent breakdowns
12
1201
6.0%
41
3.4%
1108
92.3%
65
5.4 %
48
4.0 %
4
0.3 %
Parallel - severe IRs - in persistent herds
27
196
1.0%
75
38.3%
106
54.1%
17
8.7 %
15
7.7 %
0
Wales - High TB
75
4403
22.2%
242
5.5%
3915
88.9%
181
4.1 %
130
3.0 %
116
2.6 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Parallel New Infection
9
471
2.4%
18
3.8%
402
85.4%
55
11.7 %
48
10.2 %
3
0.6 %
Parallel Persistent Infection
3
143
0.7%
5
3.5%
134
93.7%
4
2.8 %
4
2.8 %
0
Parallel - Rapid Testing Two-times IRs
2
3
0.0%
0
0.0 %
2
66.7%
1
33.3 %
1
33.3 %
0
Parallel - Recurrent breakdowns
3
378
1.9%
3
0.8%
350
92.6%
26
6.9 %
25
6.6 %
0
Parallel - severe IRs - in persistent herds
2
7
0.0%
4
57.1%
3
42.9%
0
0
0
Wales - Intermediate TB
20
1003
5.0%
31
3.1%
891
88.8%
86
8.6 %
78
7.8 %
3
0.3 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
3
45
0.2%
10
22.2%
31
68.9%
5
11.1 %
4
8.9 %
0
Parallel New Infection
10
668
3.4%
36
5.4%
584
87.4%
67
10.0 %
48
7.2 %
0
Parallel Persistent Infection
1
58
0.3%
5
8.6%
53
91.4%
0
0
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel - Recurrent breakdowns
2
189
1.0%
3
1.6%
166
87.8%
19
10.1 %
19
10.1 %
1
0.5 %
Parallel - severe IRs - in persistent herds
1
5
0.0%
1
20.0%
4
80.0%
0
0
0
Wales - Low TB
19
967
4.9%
55
5.7%
839
86.8%
92
9.5 %
72
7.4 %
1
0.1 %
Scotland - Scotland
Parallel New Infection
2
430
2.2%
7
1.6%
392
91.2%
41
9.5 %
31
7.2 %
0
Scotland - Scotland
2
430
2.2%
7
1.6%
392
91.2%
41
9.5 %
31
7.2 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
29
1920
3.1%
186
9.7%
1532
79.8%
228
11.9 %
199
10.4 %
3
0.2 %
Parallel – chronic breakdown
5
411
0.7%
51
12.4%
337
82.0%
26
6.3 %
23
5.6 %
0
Parallel - Herd or Group Slaughter
2
44
0.1%
6
13.6%
34
77.3%
4
9.1 %
4
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herds
28
112
0.2%
56
50.0%
49
43.8%
7
6.3 %
7
6.3 %
0
Parallel New Infection
117
11562
18.5%
415
3.6%
9904
85.7%
1342
11.6 %
1178
10.2 %
65
0.6 %
Parallel - new infection cull area
5
381
0.6%
26
6.8%
331
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
15
1837
2.9%
145
7.9%
1553
84.5%
164
8.9 %
138
7.5 %
1
0.1 %
Parallel Persistent Infection
104
12769
20.5%
891
7.0%
10947
85.7%
948
7.4 %
806
6.3 %
125
1.0 %
Parallel - persistent infection cull area
9
2425
3.9%
94
3.9%
1736
71.6%
112
4.6 %
99
4.1 %
496
20.5 %
Parallel - Rapid Testing Two-times IRs
61
193
0.3%
71
36.8%
112
58.0%
11
5.7 %
10
5.2 %
0
Parallel - Recurrent breakdowns
219
29567
47.4%
1394
4.7%
26011
88.0%
2088
7.1 %
1833
6.2 %
329
1.1 %
Parallel - severe IRs - in persistent herds
107
892
1.4%
345
38.7%
491
55.0%
61
6.8 %
54
6.1 %
2
0.2 %
Serial - Other
5
268
0.4%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
Total
706
62381
100.0%
3681
5.9%
53297
85.4%
5029
8.1 %
4381
7.0 %
1022
1.6 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2024)
England - HRA
Extended Flexible IFN-GAMMA test
9
165
0.3%
35
21.2%
113
68.5%
21
12.7 %
16
9.7 %
1
0.6 %
Parallel – chronic breakdown
5
411
0.7%
51
12.4%
337
82.0%
26
6.3 %
23
5.6 %
0
Parallel - new infection cull area
5
381
0.6%
26
6.8%
331
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
5
1259
2.0%
113
9.0%
1032
82.0%
130
10.3 %
114
9.1 %
0
Parallel Persistent Infection
27
3626
5.8%
333
9.2%
2990
82.5%
343
9.5 %
299
8.2 %
4
0.1 %
Parallel - persistent infection cull area
9
2425
3.9%
94
3.9%
1736
71.6%
112
4.6 %
99
4.1 %
496
20.5 %
Parallel - Recurrent breakdowns
142
19878
31.9%
1064
5.4%
17156
86.3%
1525
7.7 %
1348
6.8 %
310
1.6 %
England - Edge
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
7
77.8%
2
22.2 %
2
22.2 %
0
Parallel New Infection
47
5426
8.7%
217
4.0%
4566
84.2%
704
13.0 %
614
11.3 %
29
0.5 %
Parallel - Other
1
268
0.4%
11
4.1%
253
94.4%
5
1.9 %
4
1.5 %
0
Parallel Persistent Infection
10
1638
2.6%
103
6.3%
1360
83.0%
198
12.1 %
175
10.7 %
0
Parallel - Recurrent breakdowns
20
2780
4.5%
101
3.6%
2467
88.7%
210
7.6 %
206
7.4 %
6
0.2 %
England - LRA
Parallel New Infection
20
1842
3.0%
48
2.6%
1635
88.8%
149
8.1 %
137
7.4 %
22
1.2 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2024) Cont.
Serial - Other
5
268
0.4%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
England
306
40376
64.7%
2197
5.4%
34243
84.8%
3463
8.6 %
3067
7.6 %
869
2.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
13
1490
2.4%
101
6.8%
1231
82.6%
178
11.9 %
156
10.5 %
2
0.1 %
Parallel - Herd or Group Slaughter
1
35
0.1%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent herd
22
82
0.1%
37
45.1%
38
46.3%
7
8.5 %
7
8.5 %
0
Parallel - Other
9
310
0.5%
21
6.8%
268
86.5%
29
9.4 %
20
6.5 %
1
0.3 %
Parallel Persistent Infection
46
6118
9.8%
384
6.3%
5336
87.2%
353
5.8 %
283
4.6 %
115
1.9 %
Parallel - Rapid Testing Two-times IRs
48
147
0.2%
60
40.8%
84
57.1%
4
2.7 %
3
2.0 %
0
Parallel - Recurrent breakdowns
47
6111
9.8%
207
3.4%
5678
92.9%
285
4.7 %
214
3.5 %
12
0.2 %
Parallel - severe IRs - in persistent herds
90
818
1.3%
319
39.0%
449
54.9%
55
6.7 %
48
5.9 %
2
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
128
0.2%
21
16.4%
100
78.1%
8
6.3 %
7
5.5 %
0
Parallel - IRs in Exit Strategy for persistent herd
6
30
0.0%
19
63.3%
11
36.7%
0
0
0
Parallel New Infection
22
1797
2.9%
65
3.6%
1579
87.9%
165
9.2 %
143
8.0 %
10
0.6 %
Parallel Persistent Infection
20
1329
2.1%
66
5.0%
1208
90.9%
54
4.1 %
49
3.7 %
6
0.5 %
Parallel - Rapid Testing Two-times IRs
9
35
0.1%
8
22.9%
23
65.7%
4
11.4 %
4
11.4 %
0
Parallel - Recurrent breakdowns
8
609
1.0%
19
3.1%
544
89.3%
49
8.0 %
46
7.6 %
0
Parallel - severe IRs - in persistent herds
11
40
0.1%
12
30.0%
24
60.0%
4
10.0 %
4
10.0 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
5
137
0.2%
29
21.2%
88
64.2%
21
15.3 %
20
14.6 %
0
Parallel New Infection
22
1659
2.7%
66
4.0%
1388
83.7%
228
13.7 %
202
12.2 %
3
0.2 %
Parallel Persistent Infection
1
58
0.1%
5
8.6%
53
91.4%
0
0
0
Parallel - Rapid Testing Two-times IRs
4
11
0.0%
3
27.3%
5
45.5%
3
27.3 %
3
27.3 %
0
Parallel - Recurrent breakdowns
2
189
0.3%
3
1.6%
166
87.8%
19
10.1 %
19
10.1 %
1
0.5 %
Parallel - severe IRs - in persistent herds
6
34
0.1%
14
41.2%
18
52.9%
2
5.9 %
2
5.9 %
0
Wales
394
21167
33.9%
1465
6.9%
18318
86.5%
1470
6.9 %
1232
5.8 %
152
0.7 %
Scotland - Scotland
Parallel New Infection
6
838
1.3%
19
2.3%
736
87.8%
96
11.5 %
82
9.8 %
1
0.1 %
Scotland
6
838
1.3%
19
2.3%
736
87.8%
96
11.5 %
82
9.8 %
1
0.1 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2023 April
203
16194
118
782
1
10
0
0
0
0
53
6
0
11
0
634
0
58
1
4
May
208
13834
112
582
4
0
0
0
0
44
2
0
2
0
493
0
37
0
0
June
189
xx-xxxx-xxxx
94
524
2
0
0
0
0
33
2
0
9
0
447
0
31
0
0
July
209
18470
108
597
15
0
0
0
0
81
4
0
10
0
438
0
47
1
0
August
252
20681
140
867
7
0
0
0
0
95
6
0
19
0
675
0
65
0
0
September
221
18070
112
638
10
0
0
0
0
52
2
0
12
0
503
0
56
1
0
October
253
18656
139
825
1
7
0
0
0
0
50
2
0
9
0
701
0
53
0
2
November
250
22290
157
1277
6
24
0
0
0
0
142
8
0
18
0
982
0
97
0
0
December
183
14037
114
892
11
0
0
0
0
69
0
0
18
3
747
0
44
0
0
2024 January
234
21995
171
1438
1
27
0
0
0
0
54
3
1
5
1
1254
12
79
0
1
February
257
20510
159
1189
66
0
0
0
0
32
0
0
10
2
980
13
85
1
0
March
215
19876
142
1054
233
414
0
0
0
0
21
1
14
3
0
182
17
2
0
0
Totals (last
12 months)
2674
221094
1566
10665
242
597
0
0
0
0
726
36
15
126
6
8036
42
654
4
7
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1040
143718
701
6493
505
0
0
0
0
670
21
13
70
1
4937
1
59
1
0
Scotland
72
9847
43
273
6
0
0
0
0
31
0
0
5
0
177
1
41
1
0
Wales
1562
67529
822
3899
86
0
0
0
0
25
15
2
51
5
2922
40
554
2
7
Totals
2674
221094
1566
10665
597
0
0
0
0
726
36
15
126
6
8036
42
654
4
7
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
101
108
112
140
109
112
114
139
93
157
69
114
63
171
98
159
73
142
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2022
2023
2024
IFNg Positive herds
IFNg Negative herds
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
19561
867
17206
638
17659
825
20756
1277
13035
892
20308
1434
18508
1189
18373
1054
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2022
2023
2024
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
10
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2022
2023
2024
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
50
100
150
200
250
Wigtown
Warks
Staffs
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
Notts
Northants
NE Wales
Leics & Rut
Humberside
Heref
Gwynedd
Gwent
Gloucs
E Sussex
Dorset
Devon
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Berks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during March 2024 (% submission per
County)
County
%Resampled %Retested
Avon
6.20 %
7.55 %
Berks
100.00 %
100.00 %
Bucks
33.33 %
33.33 %
Carms
2.95 %
3.59 %
Ceredigion
Cheshire
14.00 %
15.29 %
Corn & Scilly
9.60 %
11.32 %
Cumbria
6.06 %
6.76 %
Devon
7.05 %
8.22 %
Dorset
16.22 %
18.92 %
E Sussex
2.99 %
4.48 %
Gloucs
3.04 %
3.04 %
Gwent
1.25 %
2.50 %
Gwynedd
6.56 %
8.13 %
Heref
9.32 %
11.00 %
Humberside
7.79 %
10.39 %
Leics & Rut
6.42 %
7.01 %
NE Wales
7.47 %
8.86 %
Northants
4.14 %
4.44 %
Notts
13.56 %
14.10 %
Oxon
2.72 %
3.54 %
Pembs
2.93 %
4.17 %
Powys
14.71 %
15.69 %
S Glamorgan
Shropshire
6.84 %
7.88 %
Somerset excl
5.05 %
5.81 %
Staffs
5.73 %
10.19 %
Warks
27.03 %
27.03 %
Wigtown
7.21 %
9.53 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
12853
719
5.6%
13073
719
5.5%
Wales
6144
328
5.3%
6373
328
5.1%
Scotland
430
7
1.6%
430
7
1.6%
GB
19427
1054
5.4%
19876
1054
5.3%
Table 6a (i): Number of animals and submissions tested in March 2024
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
38279
2193
5.7%
40376
2197
5.4%
Wales
20191
1465
7.3%
21167
1465
6.9%
Scotland
808
19
2.4%
838
19
2.3%
GB
59275
3677
6.2%
62381
3681
5.9%
Table 6b (i): Number of animals and submissions tested in 2024 Up to end of March
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
13783
615
4.5%
14014
615
4.4%
Wales
4634
193
4.2%
4695
193
4.1%
Scotland
1091
59
5.4%
1185
59
5.0%
GB
19508
867
4.4%
19894
867
4.4%
Table 6a (ii): Number of animals and submissions tested in March 2023
Table 6b (ii): Number of animals and submissions tested in 2023 Up to end of March
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
38874
1745
4.5%
40916
1745
4.3%
Wales
16375
1080
6.6%
17439
1080
6.2%
Scotland
1734
216
12.5%
2773
216
7.8%
GB
56966
3041
5.3%
61128
3041
5.0%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
1
1
0
0
Wiltshire
PRV-SP
1
1
0
0
Worcestershire
PRV-SE
1
1
0
0
Total
3
3
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples March 2024
Table 7b Number of private samples 2024
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
Devon
PRV-SE
1
1
0
0
East Sussex
PRV-SP
1
1
0
0
North Yorkshire
PRV-SP
1
1
0
0
Oxfordshire
PRV-SE
1
5
0
0
Wiltshire
PRV-SP
1
1
0
0
Worcestershire
PRV-SE
1
1
0
0
Total
7
11
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
2
18
4
22.2%
BOV-IR-EX
1
6
2
33.3%
Ceredigion
BOV-IR-C
1
17
1
5.9%
BOV-IR-EX
2
5
3
60.0%
North East Wales
BOV-IR-C
1
6
1
16.7%
BOV-IR-EX
1
7
1
14.3%
BOV-OTHER
1
9
5
55.6%
BOV-P
1
1
0
0
Pembrokeshire
BOV-IR-C
16
219
54
24.7%
BOV-OTHER
1
42
3
7.1%
BOV-P
1
9
1
11.1%
Powys
BOV-IR-C
3
21
4
19.0%
South Wales
BOV-IR-C
2
7
1
14.3%
BOV-IR-EX
1
16
4
25.0%
Wales
34
383
84
21.9%
All GB
34
383
84
21.9 %
Table 8a Number of Cattle Antibody Tests March 2024
Table 8b Number of Cattle Antibody Tests 2024
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cumbria
BOV-OTHER
4
885
22
2.5%
England
4
885
22
2.5%
Wales
Carmarthenshire
BOV-IR-C
13
123
27
22.0%
BOV-IR-EX
2
7
2
28.6%
BOV-OTHER
1
7
0
0
Ceredigion
BOV-IR-C
5
31
2
6.5%
BOV-IR-EX
4
28
7
25.0%
North East Wales
BOV-IR-C
4
30
8
26.7%
BOV-IR-EX
1
7
1
14.3%
BOV-OTHER
1
9
5
55.6%
BOV-P
2
53
4
7.5%
North West Wales
BOV-IR-C
1
5
0
0
Pembrokeshire
BOV-IR-C
53
564
106
18.8%
BOV-IR-EX
7
23
3
13.0%
BOV-OTHER
1
68
3
4.4%
BOV-P
2
42
6
14.3%
Powys
BOV-IR-C
8
60
8
13.3%
BOV-IR-EX
2
16
1
6.3%
South Wales
BOV-IR-C
3
12
1
8.3%
BOV-IR-EX
1
16
4
25.0%
Wales
111
1,101
188
17.1%
All GB
115
1,986
210
10.6 %