Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - December 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 160
Report Period 1st - 31st December 2019
Issued 10 January 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
December
2019
%
Total 2019
%
Submissions
234
3471
Samples
Total (%)
25192
100.00 %
331966
100.00 %
England
19595
77.78 %
263774
79.46 %
Wales
5597
22.22 %
65499
19.73 %
Scotland
2693
0.81 %
Parallel Tests
PAR-CUL-N
9518
37.78 %
75646
22.79 %
PAR-CUL-P
400
1.59 %
21742
6.55 %
PAR-HERD-S
851
3.38 %
8385
2.53 %
PAR-NEW-IN
7512
29.82 %
147126
44.32 %
PAR-OTHER
578
2.29 %
5794
1.75 %
PAR-PERSIS
5998
23.81 %
65152
19.63 %
PAR-RAPID
127
0.50 %
1512
0.46 %
Total(% of all samples)
24984
99.17 %
325357
98.01 %
Serial/Extended Tests
SER-FLEXI
208
0.83 %
6571
1.98 %
SER-FRAUD
0
0.00 %
27
0.01 %
SER-NSR
0
0.00 %
11
0.00 %
Total(% of all samples)
208
0.83 %
6609
1.99 %
Total
25192
100.00 %
331966
100.00 %
Retests
Total (% of all samples)
1500
5.95 %
22682
6.83 %
England
1202
4.77 %
18623
5.61 %
Wales
298
1.18 %
3932
1.18 %
Scotland
127
0.04 %
Resamples
Total (% of all samples)
1197
4.75 %
17635
5.31 %
England
987
3.92 %
14547
4.38 %
Wales
210
0.83 %
2984
0.90 %
Scotland
104
0.03 %
Rejects
Total (% of all samples)
1166
4.63 %
5314
1.60 %
England
1159
4.60 %
4726
1.42 %
Wales
7
0.03 %
578
0.17 %
Scotland
0
0.00 %
10
0.00 %
Table 1. Monthly Totals for December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
9518
37.8 %
PAR-CUL-P
400
1.6 %
PAR-HERD-S
851
3.4 %
PAR-NEW-IN
7512
29.8 %
PAR-OTHER
578
2.3 %
PAR-PERSIS
5998
23.8 %
PAR-RAPID
127
0.5 %
SER-FLEXI
208
0.8 %
Total
25192
100.00 %
Figure 1. Number of samples submitted in each category during December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Somerset excl N
Oxon
N Yorks
Northants
Leics & Rut
Heref
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Avon
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
10000
20000
30000
40000
50000
60000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gr London
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
4
618
2.45 %
7
1.13 %
586
94.82 %
27
4.37 %
25
4.05 %
0
0.00 %
PAR-CUL-P
1
105
0.42 %
3
2.86 %
97
92.38 %
7
6.67 %
5
4.76 %
0
0.00 %
PAR-PERSIS
4
1,702
6.76 %
71
4.17 %
1450
85.19 %
80
4.70 %
63
3.70 %
118
6.93 %
Devon
PAR-CUL-N
18
2,131
8.46 %
89
4.18 %
1809
84.89 %
212
9.95 %
186
8.73 %
47
2.21 %
PAR-PERSIS
3
405
1.61 %
13
3.21 %
378
93.33 %
18
4.44 %
14
3.46 %
0
0.00 %
Dorset
PAR-CUL-N
7
1,670
6.63 %
22
1.32 %
1587
95.03 %
70
4.19 %
60
3.59 %
1
0.06 %
PAR-CUL-P
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
SER-FLEXI
1
3
0.01 %
1
33.33 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
Gloucs
PAR-CUL-N
4
195
0.77 %
4
2.05 %
181
92.82 %
3
1.54 %
3
1.54 %
7
3.59 %
PAR-CUL-P
3
282
1.12 %
11
3.90 %
260
92.20 %
16
5.67 %
11
3.90 %
0
0.00 %
Heref
PAR-CUL-N
2
173
0.69 %
6
3.47 %
157
90.75 %
13
7.51 %
10
5.78 %
0
0.00 %
PAR-NEW-IN
1
30
0.12 %
8
26.67 %
21
70.00 %
1
3.33 %
1
3.33 %
0
0.00 %
PAR-PERSIS
2
196
0.78 %
7
3.57 %
174
88.78 %
17
8.67 %
15
7.65 %
0
0.00 %
Somerset excl N
PAR-CUL-N
3
120
0.48 %
1
0.83 %
114
95.00 %
6
5.00 %
5
4.17 %
0
0.00 %
Wiltshire
PAR-CUL-N
9
1,960
7.78 %
50
2.55 %
1491
76.07 %
79
4.03 %
62
3.16 %
357
18.21 %
PAR-CUL-P
1
11
0.04 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-OTHER
1
10
0.04 %
1
10.00 %
9
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
4
462
1.83 %
19
4.11 %
432
93.51 %
14
3.03 %
11
2.38 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
England - Edge
Bucks
PAR-NEW-IN
3
20
0.08 %
1
5.00 %
19
95.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-CUL-N
5
2,651
10.52 %
71
2.68 %
2402
90.61 %
214
8.07 %
178
6.71 %
0
0.00 %
PAR-NEW-IN
17
1,875
7.44 %
39
2.08 %
xx-xxxx-xxxx
90.56 %
154
8.21 %
128
6.83 %
10
0.53 %
PAR-PERSIS
1
32
0.13 %
3
9.38 %
20
62.50 %
9
28.13 %
9
28.13 %
0
0.00 %
Derbyshire
PAR-NEW-IN
17
1,642
6.52 %
49
2.98 %
1383
84.23 %
74
4.51 %
59
3.59 %
151
9.20 %
E Sussex
PAR-NEW-IN
1
140
0.56 %
11
7.86 %
76
54.29 %
7
5.00 %
3
2.14 %
50
35.71 %
Table 2a. Summary by County for December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
4
407
1.62 %
20
4.91 %
381
93.61 %
9
2.21 %
6
1.47 %
0
0.00 %
Northants
PAR-NEW-IN
6
1,468
5.83 %
58
3.95 %
966
65.80 %
91
6.20 %
76
5.18 %
368
25.07 %
Oxon
PAR-NEW-IN
10
932
3.70 %
32
3.43 %
813
87.23 %
54
5.79 %
37
3.97 %
50
5.36 %
PAR-OTHER
1
111
0.44 %
7
6.31 %
102
91.89 %
3
2.70 %
2
1.80 %
0
0.00 %
Warks
PAR-NEW-IN
4
117
0.46 %
17
14.53 %
95
81.20 %
7
5.98 %
5
4.27 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
85
0.34 %
1
1.18 %
77
90.59 %
8
9.41 %
7
8.24 %
0
0.00 %
N Yorks
PAR-NEW-IN
2
38
0.15 %
0
0.00 %
37
97.37 %
3
7.89 %
1
2.63 %
0
0.00 %
England
143
19595
77.78 %
623
3.18 %
xx-xxxx-xxxx
85.87 %
1202
6.13 %
987
5.04 %
1159
5.91 %
Wales - High TB
Carms
PAR-HERD-S
4
395
1.57 %
14
3.54 %
374
94.68 %
11
2.78 %
7
1.77 %
0
0.00 %
PAR-PERSIS
6
284
1.13 %
34
11.97 %
245
86.27 %
6
2.11 %
5
1.76 %
0
0.00 %
PAR-RAPID
5
24
0.10 %
5
20.83 %
16
66.67 %
3
12.50 %
3
12.50 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
213
0.85 %
9
4.23 %
202
94.84 %
5
2.35 %
2
0.94 %
0
0.00 %
PAR-RAPID
6
43
0.17 %
7
16.28 %
35
81.40 %
1
2.33 %
1
2.33 %
0
0.00 %
Gwent
PAR-PERSIS
4
273
1.08 %
20
7.33 %
242
88.64 %
15
5.49 %
8
2.93 %
3
1.10 %
SER-FLEXI
2
58
0.23 %
15
25.86 %
42
72.41 %
1
1.72 %
1
1.72 %
0
0.00 %
Pembs
PAR-HERD-S
1
25
0.10 %
2
8.00 %
23
92.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
447
1.77 %
15
3.36 %
416
93.06 %
19
4.25 %
15
3.36 %
1
0.22 %
PAR-PERSIS
11
1,440
5.72 %
46
3.19 %
1349
93.68 %
67
4.65 %
45
3.13 %
0
0.00 %
PAR-RAPID
7
27
0.11 %
8
29.63 %
18
66.67 %
1
3.70 %
1
3.70 %
0
0.00 %
SER-FLEXI
3
82
0.33 %
9
10.98 %
72
87.80 %
2
2.44 %
1
1.22 %
0
0.00 %
Powys
PAR-HERD-S
2
420
1.67 %
16
3.81 %
392
93.33 %
21
5.00 %
12
2.86 %
0
0.00 %
PAR-PERSIS
7
875
3.47 %
23
2.63 %
781
89.26 %
84
9.60 %
68
7.77 %
3
0.34 %
PAR-RAPID
2
21
0.08 %
3
14.29 %
17
80.95 %
1
4.76 %
1
4.76 %
0
0.00 %
S Wales
PAR-HERD-S
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-PERSIS
1
53
0.21 %
1
1.89 %
49
92.45 %
8
15.09 %
3
5.66 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
1
9
0.04 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-PERSIS
2
61
0.24 %
2
3.28 %
57
93.44 %
2
3.28 %
2
3.28 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
508
2.02 %
35
6.89 %
456
89.76 %
27
5.31 %
17
3.35 %
0
0.00 %
PAR-OTHER
3
9
0.04 %
0
0.00 %
7
77.78 %
4
44.44 %
2
22.22 %
0
0.00 %
PAR-PERSIS
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
12
0.05 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
SER-FLEXI
1
52
0.21 %
2
3.85 %
50
96.15 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
49
0.19 %
0
0.00 %
46
93.88 %
5
10.20 %
3
6.12 %
0
0.00 %
NE Wales
PAR-NEW-IN
2
201
0.80 %
10
4.98 %
180
89.55 %
13
6.47 %
11
5.47 %
0
0.00 %
Wales
91
5597
22.22 %
281
5.02 %
5099
91.10 %
298
5.32 %
210
3.75 %
7
0.13 %
Grand Total
Sum:
234
25192
904
21925
1500
1197
1166
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
3
431
0.13 %
9
2.09 %
407
94.43 %
23
5.34 %
15
3.48 %
0
0.00 %
PAR-OTHER
5
84
0.03 %
1
1.19 %
71
84.52 %
12
14.29 %
12
14.29 %
0
0.00 %
PAR-PERSIS
9
1,490
0.45 %
45
3.02 %
1387
93.09 %
72
4.83 %
56
3.76 %
2
0.13 %
SER-FRAUD
3
27
0.01 %
6
22.22 %
19
70.37 %
2
7.41 %
2
7.41 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
51
5,213
1.57 %
158
3.03 %
4695
90.06 %
379
7.27 %
309
5.93 %
51
0.98 %
PAR-CUL-P
13
3,310
1.00 %
49
1.48 %
3064
92.57 %
252
7.61 %
193
5.83 %
4
0.12 %
PAR-NEW-IN
7
793
0.24 %
14
1.77 %
699
88.15 %
103
12.99 %
80
10.09 %
0
0.00 %
PAR-PERSIS
41
9,947
3.00 %
408
4.10 %
8700
87.46 %
532
5.35 %
426
4.28 %
413
4.15 %
SER-FLEXI
4
43
0.01 %
0
0.00 %
41
95.35 %
2
4.65 %
2
4.65 %
0
0.00 %
Devon
PAR-CUL-N
236
30,357
9.14 %
1446
4.76 %
26813
88.33 %
2156
7.10 %
xx-xxxx-xxxx
5.41 %
457
1.51 %
PAR-CUL-P
30
7,658
2.31 %
342
4.47 %
6899
90.09 %
514
6.71 %
413
5.39 %
4
0.05 %
PAR-NEW-IN
4
674
0.20 %
106
15.73 %
501
74.33 %
86
12.76 %
67
9.94 %
0
0.00 %
PAR-OTHER
7
532
0.16 %
6
1.13 %
352
66.17 %
202
37.97 %
174
32.71 %
0
0.00 %
PAR-PERSIS
28
3,645
1.10 %
227
6.23 %
3206
87.96 %
281
7.71 %
211
5.79 %
1
0.03 %
SER-FLEXI
8
849
0.26 %
50
5.89 %
732
86.22 %
47
5.54 %
22
2.59 %
45
5.30 %
Dorset
PAR-CUL-N
106
14,444
4.35 %
674
4.67 %
12978
89.85 %
950
6.58 %
735
5.09 %
57
0.39 %
PAR-CUL-P
20
3,391
1.02 %
254
7.49 %
3019
89.03 %
160
4.72 %
118
3.48 %
0
0.00 %
PAR-NEW-IN
5
55
0.02 %
1
1.82 %
43
78.18 %
11
20.00 %
11
20.00 %
0
0.00 %
SER-FLEXI
6
387
0.12 %
21
5.43 %
353
91.21 %
16
4.13 %
13
3.36 %
0
0.00 %
Gloucs
PAR-CUL-N
63
4,539
1.37 %
169
3.72 %
3915
86.25 %
401
8.83 %
336
7.40 %
119
2.62 %
PAR-CUL-P
13
1,560
0.47 %
63
4.04 %
1375
88.14 %
156
10.00 %
122
7.82 %
0
0.00 %
PAR-HERD-S
4
33
0.01 %
0
0.00 %
31
93.94 %
3
9.09 %
2
6.06 %
0
0.00 %
PAR-NEW-IN
1
112
0.03 %
3
2.68 %
103
91.96 %
7
6.25 %
6
5.36 %
0
0.00 %
SER-FLEXI
2
30
0.01 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
17
1,897
0.57 %
75
3.95 %
xx-xxxx-xxxx
89.51 %
142
7.49 %
113
5.96 %
11
0.58 %
PAR-CUL-P
4
418
0.13 %
16
3.83 %
373
89.23 %
36
8.61 %
29
6.94 %
0
0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
3
43
0.01 %
8
18.60 %
33
76.74 %
5
11.63 %
2
4.65 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
35
3,700
1.11 %
151
4.08 %
3319
89.70 %
274
7.41 %
218
5.89 %
12
0.32 %
Shropshire
PAR-CUL-P
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
7
635
0.19 %
43
6.77 %
545
85.83 %
60
9.45 %
46
7.24 %
1
0.16 %
PAR-PERSIS
12
1,320
0.40 %
32
2.42 %
1167
88.41 %
138
10.45 %
120
9.09 %
1
0.08 %
SER-FLEXI
5
557
0.17 %
21
3.77 %
509
91.38 %
34
6.10 %
27
4.85 %
0
0.00 %
Somerset excl N
PAR-CUL-N
51
5,778
1.74 %
230
3.98 %
4771
82.57 %
369
6.39 %
246
4.26 %
531
9.19 %
PAR-OTHER
2
238
0.07 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
13
1,907
0.57 %
110
5.77 %
1510
79.18 %
105
5.51 %
69
3.62 %
218
11.43 %
Staffs
PAR-OTHER
2
140
0.04 %
3
2.14 %
125
89.29 %
15
10.71 %
12
8.57 %
0
0.00 %
PAR-PERSIS
7
1,492
0.45 %
202
13.54 %
1201
80.50 %
130
8.71 %
90
6.03 %
0
0.00 %
Wiltshire
PAR-CUL-N
78
9,058
2.73 %
301
3.32 %
7859
86.76 %
606
6.69 %
508
5.61 %
390
4.31 %
PAR-CUL-P
27
4,613
1.39 %
297
6.44 %
4119
89.29 %
286
6.20 %
194
4.21 %
3
0.07 %
PAR-NEW-IN
7
456
0.14 %
15
3.29 %
398
87.28 %
53
11.62 %
43
9.43 %
0
0.00 %
PAR-OTHER
5
607
0.18 %
6
0.99 %
591
97.36 %
19
3.13 %
10
1.65 %
0
0.00 %
PAR-PERSIS
19
4,037
1.22 %
179
4.43 %
3606
89.32 %
209
5.18 %
140
3.47 %
112
2.77 %
SER-FLEXI
4
71
0.02 %
9
12.68 %
59
83.10 %
4
5.63 %
3
4.23 %
0
0.00 %
Worcs
PAR-PERSIS
4
138
0.04 %
6
4.35 %
99
71.74 %
2
1.45 %
1
0.72 %
32
23.19 %
England - Edge
Berks
PAR-NEW-IN
39
4,468
1.35 %
155
3.47 %
4016
89.88 %
362
8.10 %
297
6.65 %
0
0.00 %
PAR-PERSIS
3
204
0.06 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
37
3,072
0.93 %
100
3.26 %
2717
88.44 %
270
8.79 %
220
7.16 %
35
1.14 %
Cheshire
PAR-CUL-N
10
4,360
1.31 %
114
2.61 %
3992
91.56 %
308
7.06 %
254
5.83 %
0
0.00 %
PAR-NEW-IN
242
48,089
14.49 %
1056
2.20 %
44290
92.10 %
2824
5.87 %
2091
4.35 %
652
1.36 %
PAR-PERSIS
6
572
0.17 %
6
1.05 %
532
93.01 %
38
6.64 %
34
5.94 %
0
0.00 %
Derbyshire
PAR-NEW-IN
191
21,184
6.38 %
659
3.11 %
18762
88.57 %
1600
7.55 %
1322
6.24 %
441
2.08 %
E Sussex
PAR-NEW-IN
22
2,861
0.86 %
136
4.75 %
2526
88.29 %
164
5.73 %
152
5.31 %
63
2.20 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-NEW-IN
37
3,029
0.91 %
71
2.34 %
2744
90.59 %
270
8.91 %
216
7.13 %
0
0.00 %
PAR-PERSIS
1
37
0.01 %
2
5.41 %
28
75.68 %
9
24.32 %
7
18.92 %
0
0.00 %
SER-NSR
2
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
62
7,032
2.12 %
314
4.47 %
6243
88.78 %
472
6.71 %
380
5.40 %
95
1.35 %
PAR-PERSIS
1
336
0.10 %
20
5.95 %
305
90.77 %
11
3.27 %
8
2.38 %
3
0.89 %
SER-NSR
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
37
3,690
1.11 %
181
4.91 %
2898
78.54 %
232
6.29 %
166
4.50 %
445
12.06 %
Notts
PAR-NEW-IN
7
774
0.23 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
164
16,269
4.90 %
643
3.95 %
14391
88.46 %
1307
8.03 %
1030
6.33 %
205
1.26 %
PAR-OTHER
1
111
0.03 %
7
6.31 %
102
91.89 %
3
2.70 %
2
1.80 %
0
0.00 %
PAR-PERSIS
2
339
0.10 %
5
1.47 %
312
92.04 %
28
8.26 %
22
6.49 %
0
0.00 %
Warks
PAR-NEW-IN
112
9,238
2.78 %
413
4.47 %
8216
88.94 %
665
7.20 %
522
5.65 %
87
0.94 %
PAR-PERSIS
6
570
0.17 %
19
3.33 %
526
92.28 %
32
5.61 %
25
4.39 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
7
409
0.12 %
22
5.38 %
355
86.80 %
30
7.33 %
24
5.87 %
8
1.96 %
Cumbria
PAR-NEW-IN
21
3,783
1.14 %
110
2.91 %
3430
90.67 %
325
8.59 %
243
6.42 %
0
0.00 %
PAR-PERSIS
1
135
0.04 %
4
2.96 %
121
89.63 %
19
14.07 %
10
7.41 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.03 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr London
PAR-NEW-IN
2
45
0.01 %
0
0.00 %
42
93.33 %
6
13.33 %
3
6.67 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
3
164
0.05 %
7
4.27 %
129
78.66 %
33
20.12 %
28
17.07 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.30 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
4
641
0.19 %
16
2.50 %
562
87.68 %
69
10.76 %
62
9.67 %
1
0.16 %
Kent
PAR-NEW-IN
1
10
0.00 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
106
0.03 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
12
1,350
0.41 %
33
2.44 %
1202
89.04 %
128
9.48 %
115
8.52 %
0
0.00 %
Norfolk
PAR-NEW-IN
1
41
0.01 %
1
2.44 %
38
92.68 %
2
4.88 %
2
4.88 %
0
0.00 %
Northumb
PAR-NEW-IN
3
296
0.09 %
0
0.00 %
241
81.42 %
63
21.28 %
55
18.58 %
0
0.00 %
PAR-PERSIS
3
183
0.06 %
1
0.55 %
32
17.49 %
58
31.69 %
57
31.15 %
93
50.82 %
N Yorks
PAR-NEW-IN
9
913
0.28 %
22
2.41 %
803
87.95 %
74
8.11 %
60
6.57 %
28
3.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
3
575
0.17 %
2
0.35 %
551
95.83 %
31
5.39 %
22
3.83 %
0
0.00 %
SER-FLEXI
2
50
0.02 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
Suffolk
PAR-NEW-IN
3
334
0.10 %
3
0.90 %
291
87.13 %
45
13.47 %
40
11.98 %
0
0.00 %
S Yorks
PAR-NEW-IN
5
404
0.12 %
7
1.73 %
381
94.31 %
21
5.20 %
15
3.71 %
1
0.25 %
W Sussex
PAR-NEW-IN
2
239
0.07 %
2
0.84 %
163
68.20 %
81
33.89 %
74
30.96 %
0
0.00 %
W Yorks
PAR-HERD-S
2
24
0.01 %
8
33.33 %
14
58.33 %
2
8.33 %
2
8.33 %
0
0.00 %
England
2054
263774
79.46 %
10026
3.80 %
234494
88.90 %
18623
7.06 %
14547
5.51 %
4726
1.79 %
Wales - High TB
Carms
PAR-HERD-S
63
3,721
1.12 %
248
6.66 %
3283
88.23 %
248
6.66 %
188
5.05 %
2
0.05 %
PAR-NEW-IN
3
121
0.04 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
16
464
0.14 %
70
15.09 %
384
82.76 %
20
4.31 %
10
2.16 %
0
0.00 %
PAR-PERSIS
79
6,894
2.08 %
393
5.70 %
6240
90.51 %
337
4.89 %
250
3.63 %
13
0.19 %
PAR-RAPID
87
389
0.12 %
96
24.68 %
272
69.92 %
34
8.74 %
21
5.40 %
0
0.00 %
SER-FLEXI
43
908
0.27 %
92
10.13 %
775
85.35 %
51
5.62 %
41
4.52 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.04 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-NEW-IN
2
313
0.09 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
3
160
0.05 %
5
3.13 %
148
92.50 %
10
6.25 %
7
4.38 %
0
0.00 %
PAR-PERSIS
36
2,780
0.84 %
162
5.83 %
2477
89.10 %
189
6.80 %
140
5.04 %
1
0.04 %
PAR-RAPID
57
172
0.05 %
33
19.19 %
134
77.91 %
7
4.07 %
5
2.91 %
0
0.00 %
SER-FLEXI
10
533
0.16 %
18
3.38 %
501
94.00 %
15
2.81 %
12
2.25 %
2
0.38 %
Gwent
PAR-HERD-S
11
887
0.27 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
3
30
0.01 %
6
20.00 %
24
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
24
1,684
0.51 %
96
5.70 %
1513
89.85 %
99
5.88 %
71
4.22 %
4
0.24 %
PAR-RAPID
28
83
0.03 %
19
22.89 %
55
66.27 %
9
10.84 %
8
9.64 %
1
1.20 %
SER-FLEXI
4
82
0.02 %
17
20.73 %
64
78.05 %
1
1.22 %
1
1.22 %
0
0.00 %
Pembs
PAR-HERD-S
37
2,406
0.72 %
81
3.37 %
2264
94.10 %
107
4.45 %
59
2.45 %
3
0.12 %
PAR-NEW-IN
3
8
0.00 %
2
25.00 %
4
50.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-OTHER
22
1,884
0.57 %
86
4.56 %
xx-xxxx-xxxx
89.44 %
141
7.48 %
112
5.94 %
1
0.05 %
PAR-PERSIS
146
15,340
4.62 %
506
3.30 %
14055
91.62 %
989
6.45 %
771
5.03 %
8
0.05 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
133
366
0.11 %
71
19.40 %
268
73.22 %
21
5.74 %
14
3.83 %
13
3.55 %
SER-FLEXI
50
1,267
0.38 %
93
7.34 %
1123
88.63 %
69
5.45 %
50
3.95 %
1
0.08 %
Powys
PAR-HERD-S
28
938
0.28 %
71
7.57 %
825
87.95 %
58
6.18 %
38
4.05 %
4
0.43 %
PAR-NEW-IN
30
981
0.30 %
43
4.38 %
901
91.85 %
44
4.49 %
36
3.67 %
1
0.10 %
PAR-OTHER
2
15
0.00 %
4
26.67 %
9
60.00 %
3
20.00 %
2
13.33 %
0
0.00 %
PAR-PERSIS
49
3,256
0.98 %
111
3.41 %
2755
84.61 %
219
6.73 %
177
5.44 %
213
6.54 %
PAR-RAPID
66
204
0.06 %
27
13.24 %
138
67.65 %
15
7.35 %
14
6.86 %
25
12.25 %
SER-FLEXI
14
388
0.12 %
25
6.44 %
356
91.75 %
10
2.58 %
7
1.80 %
0
0.00 %
S Wales
PAR-HERD-S
2
5
0.00 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.00 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.12 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
2
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.03 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.04 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-OTHER
1
13
0.00 %
4
30.77 %
9
69.23 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
60
0.02 %
1
1.67 %
54
90.00 %
10
16.67 %
5
8.33 %
0
0.00 %
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
84
0.03 %
14
16.67 %
67
79.76 %
4
4.76 %
3
3.57 %
0
0.00 %
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.11 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
11
903
0.27 %
50
5.54 %
825
91.36 %
46
5.09 %
28
3.10 %
0
0.00 %
PAR-RAPID
15
29
0.01 %
3
10.34 %
24
82.76 %
2
6.90 %
2
6.90 %
0
0.00 %
SER-FLEXI
1
20
0.01 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.00 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
2
148
0.04 %
21
14.19 %
111
75.00 %
17
11.49 %
16
10.81 %
0
0.00 %
PAR-PERSIS
4
550
0.17 %
26
4.73 %
500
90.91 %
28
5.09 %
24
4.36 %
0
0.00 %
PAR-RAPID
5
14
0.00 %
8
57.14 %
6
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
27
0.01 %
2
7.41 %
21
77.78 %
4
14.81 %
4
14.81 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Mid Glamorgan
PAR-RAPID
1
6
0.00 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
93
8,778
2.64 %
265
3.02 %
7933
90.37 %
510
5.81 %
382
4.35 %
198
2.26 %
PAR-OTHER
16
332
0.10 %
21
6.33 %
291
87.65 %
25
7.53 %
20
6.02 %
0
0.00 %
PAR-PERSIS
11
1,619
0.49 %
40
2.47 %
1529
94.44 %
87
5.37 %
59
3.64 %
0
0.00 %
PAR-RAPID
18
183
0.06 %
10
5.46 %
167
91.26 %
6
3.28 %
6
3.28 %
0
0.00 %
SER-FLEXI
44
1,058
0.32 %
76
7.18 %
869
82.14 %
53
5.01 %
43
4.06 %
70
6.62 %
Powys
PAR-NEW-IN
13
1,548
0.47 %
37
2.39 %
1432
92.51 %
95
6.14 %
79
5.10 %
0
0.00 %
PAR-PERSIS
2
21
0.01 %
2
9.52 %
17
80.95 %
2
9.52 %
2
9.52 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
9
42
0.01 %
5
11.90 %
37
88.10 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
111
0.03 %
12
10.81 %
98
88.29 %
2
1.80 %
1
0.90 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
165
0.05 %
10
6.06 %
144
87.27 %
13
7.88 %
11
6.67 %
0
0.00 %
PAR-PERSIS
1
9
0.00 %
1
11.11 %
6
66.67 %
3
33.33 %
2
22.22 %
0
0.00 %
S Wales
PAR-RAPID
2
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.00 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
258
0.08 %
17
6.59 %
220
85.27 %
29
11.24 %
21
8.14 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
19
865
0.26 %
22
2.54 %
796
92.02 %
69
7.98 %
50
5.78 %
1
0.12 %
PAR-OTHER
1
17
0.01 %
0
0.00 %
17
100.00 %
1
5.88 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
16
661
0.20 %
18
2.72 %
601
90.92 %
38
5.75 %
27
4.08 %
15
2.27 %
PAR-OTHER
2
18
0.01 %
1
5.56 %
15
83.33 %
3
16.67 %
2
11.11 %
0
0.00 %
PAR-PERSIS
2
429
0.13 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
89
0.03 %
4
4.49 %
82
92.13 %
4
4.49 %
3
3.37 %
0
0.00 %
Wales
1395
65499
19.73 %
3198
4.88 %
58755
89.70 %
3932
6.00 %
2984
4.56 %
578
0.88 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.01 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ayrshire
PAR-NEW-IN
3
366
0.11 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
5
757
0.23 %
10
1.32 %
708
93.53 %
36
4.76 %
29
3.83 %
10
1.32 %
Kirkcudbright
PAR-CUL-P
1
352
0.11 %
7
1.99 %
340
96.59 %
8
2.27 %
5
1.42 %
0
0.00 %
PAR-NEW-IN
6
421
0.13 %
55
13.06 %
348
82.66 %
20
4.75 %
18
4.28 %
0
0.00 %
PAR-PERSIS
1
338
0.10 %
2
0.59 %
329
97.34 %
7
2.07 %
7
2.07 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
420
0.13 %
11
2.62 %
381
90.71 %
36
8.57 %
28
6.67 %
0
0.00 %
Scotland
22
2693
0.81 %
90
3.34 %
2489
92.42 %
127
4.72 %
104
3.86 %
10
0.37 %
Grand Total
Sum:
xx-xxxx-xxxx
13314
295738
22682
17635
5314
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol December 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
141
19591
100.0%
622
3.2%
xx-xxxx-xxxx
85.9%
1200
6.1%
986
5.0%
1159
5.9%
Serial/Extended
2
4
0.0%
1
25.0%
2
50.0%
2
50.0%
1
25.0%
0
0.0 %
Total
143
19595
100.0%
623
3.2%
xx-xxxx-xxxx
85.9%
1202
6.1%
987
5.0%
1159
5.9%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
84
5393
96.4%
252
4.7%
4926
91.3%
295
5.5%
208
3.9%
7
0.1%
Serial/Extended
7
204
3.6%
29
14.2%
173
84.8%
3
1.5%
2
1.0%
0
0.0 %
Total
91
5597
100.0%
281
5.0%
5099
91.1%
298
5.3%
210
3.8%
7
0.1%
GB
Parallel
225
24984
99.2%
874
3.5%
21750
87.1%
1495
6.0%
1194
4.8%
1166
4.7%
Serial/Extended
9
208
0.8%
30
14.4%
175
84.1%
5
2.4%
3
1.4%
0
0.0 %
Total
234
25192
100.0%
904
3.6%
21925
87.0%
1500
6.0%
1197
4.8%
1166
4.6%
143.00
91.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.2%
9916
3.8% 232694
88.9%
18513
7.1%
14477
5.5%
4681
1.8%
Serial/Extended
37
2025
0.8%
110
5.4%
1800
88.9%
110
5.4%
70
3.5%
45
2.2%
Total
xx-xxxx-xxxx
100.0% 10026
3.8% 234494
88.9%
18623
7.1%
14547
5.5%
4726
1.8%
Scotland
Parallel
22
2693
100.0%
90
3.3%
2489
92.4%
127
4.7%
104
3.9%
10
0.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
22
2693
100.0%
90
3.3%
2489
92.4%
127
4.7%
104
3.9%
10
0.4%
Wales
Parallel
1218
60915
93.0%
2842
4.7%
54766
89.9%
3718
6.1%
2818
4.6%
505
0.8%
Serial/Extended
177
4584
7.0%
356
7.8%
3989
87.0%
214
4.7%
166
3.6%
73
1.6%
Total
1395
65499
100.0%
3198
4.9%
58755
89.7%
3932
6.0%
2984
4.6%
578
0.9%
GB
Parallel
xx-xxxx-xxxx
98.0% 12848
3.9% 289949
89.1%
22358
6.9%
17399
5.3%
5196
1.6%
Serial/Extended
214
6609
2.0%
466
7.1%
5789
87.6%
324
4.9%
236
3.6%
118
1.8%
Total
xx-xxxx-xxxx
100.0% 13314
4.0% 295738
89.1%
22682
6.8%
17635
5.3%
5314
1.6%
#######
#######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
208
0.8%
30
14.4%
175
84.1%
5
2.4 %
3
1.4 %
0
Parallel - Herd or Group Slaughter
9
851
3.4%
32
3.8%
799
93.9%
33
3.9 %
20
2.4 %
0
Parallel New Infection
77
7512
29.8%
281
3.7%
6248
83.2%
453
6.0 %
354
4.7 %
629
8.4 %
Parallel - new infection cull area
52
9518
37.8%
250
2.6%
8327
87.5%
624
6.6 %
529
5.6 %
412
4.3 %
Parallel - Other
9
578
2.3%
23
4.0%
535
92.6%
26
4.5 %
19
3.3 %
1
0.2 %
Parallel Persistent Infection
47
5998
23.8%
249
4.2%
5380
89.7%
325
5.4 %
245
4.1 %
124
2.1 %
Parallel - persistent infection cull area
6
400
1.6%
15
3.8%
365
91.3%
27
6.8 %
20
5.0 %
0
Parallel - Rapid Testing Two-times IRs
25
127
0.5%
24
18.9%
96
75.6%
7
5.5 %
7
5.5 %
0
Total
234
25192
100.0%
904
3.6%
21925
87.0%
1500
6.0 %
1197
4.8 %
1166
4.6 %
Table 4a. Summaryof IFN Gamma results by submission reason (December 2019)
England - HRA
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
2
50.0%
2
50.0 %
1
25.0 %
0
Parallel New Infection
1
30
0.1%
8
26.7%
21
70.0%
1
3.3 %
1
3.3 %
0
Parallel - new infection cull area
47
6867
27.3%
179
2.6%
5925
86.3%
410
6.0 %
351
5.1 %
412
6.0 %
Parallel - Other
2
11
0.0%
1
9.1%
10
90.9%
0
0
0
Parallel Persistent Infection
13
2765
11.0%
110
4.0%
2434
88.0%
129
4.7 %
103
3.7 %
118
4.3 %
Parallel - persistent infection cull area
6
400
1.6%
15
3.8%
365
91.3%
27
6.8 %
20
5.0 %
0
England - HRA
71
10077
40.0%
314
3.1%
8757
86.9%
569
5.6 %
476
4.7 %
530
5.3 %
England - Edge
Parallel New Infection
62
6601
26.2%
227
3.4%
5431
82.3%
396
6.0 %
314
4.8 %
629
9.5 %
Parallel - new infection cull area
5
2651
10.5%
71
2.7%
2402
90.6%
214
8.1 %
178
6.7 %
0
Parallel - Other
1
111
0.4%
7
6.3%
102
91.9%
3
2.7 %
2
1.8 %
0
Parallel Persistent Infection
1
32
0.1%
3
9.4%
20
62.5%
9
28.1 %
9
28.1 %
0
England - Edge
69
9395
37.3%
308
3.3%
7955
84.7%
622
6.6 %
503
5.4 %
629
6.7 %
England - LRA
Parallel New Infection
3
123
0.5%
1
0.8%
114
92.7%
11
8.9 %
8
6.5 %
0
England - LRA
3
123
0.5%
1
0.8%
114
92.7%
11
8.9 %
8
6.5 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
5
140
0.6%
24
17.1%
114
81.4%
3
2.1 %
2
1.4 %
0
Parallel - Herd or Group Slaughter
8
842
3.3%
32
3.8%
791
93.9%
32
3.8 %
19
2.3 %
0
Parallel - Other
3
447
1.8%
15
3.4%
416
93.1%
19
4.3 %
15
3.4 %
1
0.2 %
Parallel Persistent Infection
30
3138
12.5%
133
4.2%
2868
91.4%
185
5.9 %
131
4.2 %
6
0.2 %
Parallel - Rapid Testing Two-times IRs
21
117
0.5%
23
19.7%
88
75.2%
6
5.1 %
6
5.1 %
0
Wales - High TB
67
4684
18.6%
227
4.8%
4277
91.3%
245
5.2 %
173
3.7 %
7
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
64
0.3%
5
7.8%
59
92.2%
0
0
0
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Parallel New Infection
7
508
2.0%
35
6.9%
456
89.8%
27
5.3 %
17
3.3 %
0
Parallel - Other
3
9
0.0%
0
0.0 %
7
77.8%
4
44.4 %
2
22.2 %
0
Parallel Persistent Infection
3
63
0.3%
3
4.8%
58
92.1%
2
3.2 %
2
3.2 %
0
Parallel - Rapid Testing Two-times IRs
4
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Wales - Intermediate TB
20
663
2.6%
44
6.6%
596
89.9%
35
5.3 %
23
3.5 %
0
Wales - Low TB
Parallel New Infection
4
250
1.0%
10
4.0%
226
90.4%
18
7.2 %
14
5.6 %
0
Wales - Low TB
4
250
1.0%
10
4.0%
226
90.4%
18
7.2 %
14
5.6 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
208
6571
2.0%
460
7.0%
5759
87.6%
322
4.9 %
234
3.6 %
118
1.8 %
Parallel - Herd or Group Slaughter
159
8385
2.5%
494
5.9%
7490
89.3%
536
6.4 %
391
4.7 %
11
0.1 %
Parallel New Infection
1264 147126
44.3%
4700
3.2%
131936
89.7%
10381
7.1 %
8162
5.5 %
2350
1.6 %
Parallel - new infection cull area
612
75646
22.8%
3167
4.2%
66721
88.2%
5311
7.0 %
4142
5.5 %
1616
2.1 %
Parallel - Other
108
5794
1.7%
332
5.7%
4973
85.8%
555
9.6 %
446
7.7 %
43
0.7 %
Parallel Persistent Infection
568
65152
19.6%
2843
4.4%
58098
89.2%
4044
6.2 %
3097
4.8 %
1126
1.7 %
Parallel - persistent infection cull area
112
21742
6.5%
1038
4.8%
19604
90.2%
1435
6.6 %
1089
5.0 %
11
0.1 %
Parallel - Rapid Testing Two-times IRs
434
1512
0.5%
274
18.1%
1127
74.5%
96
6.3 %
72
4.8 %
39
2.6 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
13314
4.0%
295738
89.1%
22682
6.8 %
17635
5.3 %
5314
1.6 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
29
1937
0.6%
103
5.3%
1722
88.9%
105
5.4 %
67
3.5 %
45
2.3 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
28
2134
0.6%
147
6.9%
1778
83.3%
265
12.4 %
209
9.8 %
0
Parallel - new infection cull area
602
71286
21.5%
3053
4.3%
62729
88.0%
5003
7.0 %
3888
5.5 %
1616
2.3 %
Parallel - Other
30
2238
0.7%
93
4.2%
1841
82.3%
316
14.1 %
262
11.7 %
42
1.9 %
Parallel Persistent Infection
168
27676
8.3%
1360
4.9%
24195
87.4%
1743
6.3 %
1331
4.8 %
791
2.9 %
Parallel - persistent infection cull area
111
21390
6.4%
1031
4.8%
19264
90.1%
1427
6.7 %
1084
5.1 %
11
0.1 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
England - Edge
Parallel New Infection
950 119706
36.1%
3764
3.1%
107421
89.7%
8235
6.9 %
6452
5.4 %
2087
1.7 %
Parallel - new infection cull area
10
4360
1.3%
114
2.6%
3992
91.6%
308
7.1 %
254
5.8 %
0
Parallel - Other
1
111
0.0%
7
6.3%
102
91.9%
3
2.7 %
2
1.8 %
0
Parallel Persistent Infection
19
2058
0.6%
57
2.8%
1899
92.3%
123
6.0 %
99
4.8 %
3
0.1 %
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
85
9840
3.0%
275
2.8%
8724
88.7%
977
9.9 %
803
8.2 %
38
0.4 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Parallel Persistent Infection
7
893
0.3%
7
0.8%
704
78.8%
108
12.1 %
89
10.0 %
93
10.4 %
England
xx-xxxx-xxxx
79.5%
10026
3.8%
234494
88.9%
18623
7.1 %
14547
5.5 %
4726
1.8 %
Wales - High TB
Extended Flexible IFN-GAMMA test
124
3279
1.0%
250
7.6%
2911
88.8%
151
4.6 %
115
3.5 %
3
0.1 %
Parallel - Herd or Group Slaughter
146
8218
2.5%
469
5.7%
7365
89.6%
517
6.3 %
374
4.6 %
11
0.1 %
Parallel New Infection
38
1423
0.4%
81
5.7%
1284
90.2%
67
4.7 %
57
4.0 %
1
0.1 %
Parallel - Other
48
2577
0.8%
175
6.8%
2270
88.1%
174
6.8 %
131
5.1 %
1
0.0 %
Parallel Persistent Infection
339
30398
9.2%
1274
4.2%
27465
90.4%
1851
6.1 %
1422
4.7 %
239
0.8 %
Parallel - Rapid Testing Two-times IRs
376
1224
0.4%
246
20.1%
876
71.6%
87
7.1 %
63
5.1 %
39
3.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
51
1216
0.4%
102
8.4%
996
81.9%
59
4.9 %
48
3.9 %
70
5.8 %
Parallel - Herd or Group Slaughter
7
110
0.0%
17
15.5%
80
72.7%
14
12.7 %
13
11.8 %
0
Parallel New Infection
108
10494
3.2%
312
3.0%
9512
90.6%
618
5.9 %
472
4.5 %
198
1.9 %
Parallel - Other
26
833
0.3%
56
6.7%
728
87.4%
58
7.0 %
49
5.9 %
0
Parallel Persistent Infection
32
3360
1.0%
136
4.0%
3097
92.2%
195
5.8 %
136
4.0 %
0
Parallel - Rapid Testing Two-times IRs
54
283
0.1%
28
9.9%
246
86.9%
9
3.2 %
9
3.2 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
35
1526
0.5%
40
2.6%
1397
91.5%
107
7.0 %
77
5.0 %
16
1.0 %
Parallel - Other
3
35
0.0%
1
2.9%
32
91.4%
4
11.4 %
2
5.7 %
0
Parallel Persistent Infection
2
429
0.1%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
4
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Wales
1395
65499
19.7%
3198
4.9%
58755
89.7%
3932
6.0 %
2984
4.6 %
578
0.9 %
Scotland - Scotland
Parallel New Infection
20
2003
0.6%
81
4.0%
1820
90.9%
112
5.6 %
92
4.6 %
10
0.5 %
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Parallel - persistent infection cull area
1
352
0.1%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland
22
2693
0.8%
90
3.3%
2489
92.4%
127
4.7 %
104
3.9 %
10
0.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
1
0
4
0
773
0
73
0
0
July
316
32549
201
1219
1
13
0
0
0
0
108
3
0
12
0
1020
0
58
0
2
August
262
21887
126
724
1
8
0
0
0
0
84
1
0
6
0
570
0
52
0
0
September
245
25317
134
693
1
7
0
0
0
0
94
0
0
3
1
542
0
45
0
0
October
298
26690
154
743
1
0
0
0
0
58
0
1
4
1
603
25
49
0
0
November
272
30186
169
1080
19
0
0
0
0
72
0
20
0
0
907
60
0
0
0
December
234
25192
138
904
31
504
0
0
0
0
22
0
3
0
0
296
19
0
0
0
Totals (last
12 months)
3471
331966
2013
13314
47
587
0
0
0
0
1105
16
24
55
2
10729
110
583
2
9
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2054
263774
1303
10026
442
0
0
0
0
969
9
16
37
1
8434
7
82
0
1
Scotland
22
2693
11
90
0
0
0
0
0
12
0
0
2
0
70
0
5
0
0
Wales
1395
65499
699
3198
145
0
0
0
0
124
7
8
16
1
2225
103
496
2
8
Totals
3471
331966
2013
13314
587
0
0
0
0
1105
16
24
55
2
10729
110
583
2
9
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2018
2019
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
Wiltshire
W Glamorgan
Warks
S Wales
Somerset excl N
S Glamorgan
Powys
Pembs
Oxon
N Yorks
Northants
NE Wales
Mid Glamorgan
Leics & Rut
Heref
Gwynedd
Gwent
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during December 2019 (% submission per
County)
County
%Resampled %Retested
Avon
Bucks
Carms
2.45 %
3.10 %
Ceredigion
1.17 %
2.34 %
Cheshire
6.91 %
8.27 %
Corn & Scilly
3.84 %
4.70 %
Derbyshire
3.59 %
4.51 %
Devon
7.89 %
9.07 %
Dorset
3.64 %
4.30 %
E Sussex
2.14 %
5.00 %
Gloucs
2.94 %
3.98 %
Gr Manchester
8.24 %
9.41 %
Gwent
2.72 %
4.83 %
Gwynedd
6.12 %
10.20 %
Heref
6.52 %
7.77 %
Leics & Rut
1.47 %
2.21 %
Mid Glamorgan
NE Wales
4.08 %
5.99 %
Northants
5.18 %
6.20 %
N Yorks
2.63 %
7.89 %
Oxon
3.74 %
5.47 %
Pembs
3.07 %
4.40 %
Powys
6.16 %
8.05 %
S Glamorgan
Somerset excl
4.17 %
5.00 %
S Wales
Warks
4.27 %
5.98 %
W Glamorgan
5.66 %
15.09 %
Wiltshire
3.15 %
3.97 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18769
623
3.3%
19595
623
3.2%
Wales
5491
281
5.1%
5597
281
5.0%
GB
24260
904
3.7%
25192
904
3.6%
Table 6a (i): Number of animals and submissions tested in December 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
230030
10026
4.4%
263774
10026
3.8%
Wales
59269
3198
5.4%
65499
3198
4.9%
Scotland
2015
90
4.5%
2693
90
3.3%
GB
291304
13314
4.6%
331966
13314
4.0%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
15080
824
5.5%
15274
824
5.4%
Wales
6199
417
6.7%
6280
417
6.6%
GB
21279
1241
5.8%
21554
1241
5.8%
Table 6a (ii): Number of animals and submissions tested in December 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
165901
9620
5.8%
193638
9620
5.0%
Wales
50414
2858
5.7%
57607
2869
5.0%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
218375
12659
5.8%
253486
12670
5.0%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
2
0
0
Dorset
PRV-SP
2
6
0
0
Worcestershire
PRV-SE
1
16
0
0
Total
4
24
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples December 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
2
7
0
0
PRV-SP
4
5
0
0
Dorset
PRV-SP
4
10
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SP
2
2
0
0
Somerset excl North
PRV-SE
6
247
16
6.48 %
PRV-SP
7
12
0
0
Staffordshire
PRV-SE
1
64
3
4.69 %
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
PRV-SE
1
16
0
0
SER-OTHER
1
5
0
0
Total
31
377
19
5.04 %
Isle of Man
SER-IOM
27
370
2
0.54 %

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
22
7
31.8%
Cornwall and Isles of
Scilly
1
26
0
0
North East Wales
1
17
2
11.8%
Pembrokeshire
3
74
13
17.6%
Powys
3
60
4
6.7%
TC0077
9
199
26
13.1 %
Table 8a Number of Cattle Antibody Tests December 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
17
597
60
10.1%
Ceredigion
3
372
17
4.6%
Cornwall and Isles of
Scilly
2
263
30
11.4%
North East Wales
30
974
119
12.2%
Pembrokeshire
24
841
78
9.3%
Powys
12
440
31
7.0%
South Wales
5
196
16
8.2%
Wiltshire
1
248
9
3.6%
TC0077
94
3,931
360
9.2 %
Table 8b Number of Cattle Antibody Tests 2019