Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - November 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 183
Report Period 1st - 30th November 2021
Issued 10 December 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
November
2021
%
Total 2021
%
Submissions
329
3714
Samples
Total (%)
25737
100.00 %
280254
100.00 %
England
20481
79.58 %
217128
77.48 %
Wales
4399
17.09 %
61103
21.80 %
Scotland
857
3.33 %
2023
0.72 %
Parallel Tests
PAR-CUL-N
2902
11.28 %
109608
39.11 %
PAR-CUL-P
2425
9.42 %
11950
4.26 %
PAR-HERD-S
2
0.01 %
1633
0.58 %
PAR-IR-EX
81
0.31 %
425
0.15 %
PAR-IR-SEV
225
0.87 %
2611
0.93 %
PAR-NEW-IN
4819
18.72 %
77294
27.58 %
PAR-OTHER
1553
6.03 %
9248
3.30 %
PAR-PERSIS
4240
xx-xxxx-xxxx %
36759
13.12 %
PAR-POSTM
46
0.18 %
281
0.10 %
PAR-RAPID
152
0.59 %
1081
0.39 %
PAR-RECUR
8549
33.22 %
23143
8.26 %
Total(% of all samples)
24994
97.11 %
274033
97.78 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
26
0.01 %
SER-FLEXI
743
2.89 %
6132
2.19 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
59
0.02 %
Total(% of all samples)
743
2.89 %
6221
2.22 %
Total
25737
100.00 %
280254
100.00 %
Retests
Total (% of all samples)
2332
9.06 %
20509
7.32 %
England
1818
7.06 %
16321
5.82 %
Wales
439
1.71 %
4027
1.44 %
Scotland
75
0.29 %
161
0.06 %
Resamples
Total (% of all samples)
2009
7.81 %
xx-xxxx-xxxx
6.00 %
England
1579
6.14 %
13490
4.81 %
Wales
366
1.42 %
3201
1.14 %
Scotland
64
0.25 %
137
0.05 %
Rejects
Total (% of all samples)
137
0.53 %
894
0.32 %
England
114
0.44 %
729
0.26 %
Wales
21
0.08 %
153
0.05 %
Scotland
2
0.01 %
12
0.00 %
Table 1. Monthly Totals for November 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR IR EX
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
2902
11.3 %
PAR-CUL-P
2425
9.4 %
PAR-HERD-S
2
0.0 %
PAR-IR-EX
81
0.3 %
PAR-IR-SEV
225
0.9 %
PAR-NEW-IN
4819
18.7 %
PAR-OTHER
1553
6.0 %
PAR-PERSIS
4240
16.5 %
PAR-POSTM
46
0.2 %
PAR-RAPID
152
0.6 %
PAR-RECUR
8549
33.2 %
SER-FLEXI
743
2.9 %
Total
25737
100.00 %
Figure 1. Number of samples submitted in each category during November 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
Wigtown
Ayrshire
W Glamorgan
S Wales
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during November 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
Wigtown
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
PAR-CUL-P
1
264
1.03 %
17
6.44 %
243
92.05 %
20
7.58 %
4
1.52 %
0
0.00 %
PAR-OTHER
1
7
0.03 %
2
28.57 %
2
28.57 %
3
42.86 %
3
42.86 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
3
155
0.60 %
8
5.16 %
58
37.42 %
17
10.97 %
16
10.32 %
73
47.10 %
PAR-OTHER
5
558
2.17 %
79
14.16 %
431
77.24 %
28
5.02 %
24
4.30 %
24
4.30 %
PAR-RECUR
4
603
2.34 %
15
2.49 %
543
90.05 %
53
8.79 %
45
7.46 %
0
0.00 %
SER-FLEXI
1
11
0.04 %
2
18.18 %
2
18.18 %
7
63.64 %
7
63.64 %
0
0.00 %
Derbyshire
PAR-RECUR
1
515
2.00 %
10
1.94 %
476
92.43 %
30
5.83 %
29
5.63 %
0
0.00 %
Devon
PAR-CUL-N
6
361
1.40 %
34
9.42 %
289
80.06 %
41
11.36 %
38
10.53 %
0
0.00 %
PAR-CUL-P
1
47
0.18 %
3
6.38 %
39
82.98 %
5
10.64 %
4
8.51 %
1
2.13 %
PAR-PERSIS
3
438
1.70 %
20
4.57 %
392
89.50 %
35
7.99 %
26
5.94 %
0
0.00 %
PAR-RECUR
10
2,451
9.52 %
74
3.02 %
2202
89.84 %
197
8.04 %
174
7.10 %
1
0.04 %
Dorset
PAR-CUL-N
1
401
1.56 %
33
8.23 %
354
88.28 %
17
4.24 %
14
3.49 %
0
0.00 %
PAR-OTHER
2
107
0.42 %
8
7.48 %
95
88.79 %
4
3.74 %
4
3.74 %
0
0.00 %
Gloucs
PAR-CUL-N
2
177
0.69 %
3
1.69 %
163
92.09 %
16
9.04 %
11
6.21 %
0
0.00 %
PAR-PERSIS
1
350
1.36 %
32
9.14 %
304
86.86 %
18
5.14 %
13
3.71 %
1
0.29 %
PAR-RECUR
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Heref
PAR-CUL-P
1
271
1.05 %
18
6.64 %
243
89.67 %
15
5.54 %
10
3.69 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-PERSIS
4
868
3.37 %
31
3.57 %
418
48.16 %
432
49.77 %
419
48.27 %
0
0.00 %
PAR-RECUR
9
778
3.02 %
32
4.11 %
702
90.23 %
49
6.30 %
43
5.53 %
1
0.13 %
Shropshire
PAR-NEW-IN
2
233
0.91 %
13
5.58 %
212
90.99 %
10
4.29 %
8
3.43 %
0
0.00 %
PAR-OTHER
1
13
0.05 %
1
7.69 %
9
69.23 %
3
23.08 %
3
23.08 %
0
0.00 %
PAR-PERSIS
1
242
0.94 %
19
7.85 %
211
87.19 %
15
6.20 %
12
4.96 %
0
0.00 %
PAR-RECUR
7
613
2.38 %
23
3.75 %
553
90.21 %
43
7.01 %
37
6.04 %
0
0.00 %
Somerset excl N
PAR-CUL-N
4
893
3.47 %
42
4.70 %
811
90.82 %
45
5.04 %
40
4.48 %
0
0.00 %
Table 2a. Summary by County for November 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
6
1,811
7.04 %
74
4.09 %
xx-xxxx-xxxx
92.77 %
65
3.59 %
48
2.65 %
9
0.50 %
PAR-RECUR
11
1,787
6.94 %
66
3.69 %
1626
90.99 %
117
6.55 %
94
5.26 %
1
0.06 %
Staffs
PAR-CUL-N
4
518
2.01 %
24
4.63 %
468
90.35 %
35
6.76 %
26
5.02 %
0
0.00 %
PAR-OTHER
2
188
0.73 %
5
2.66 %
157
83.51 %
25
13.30 %
26
13.83 %
0
0.00 %
PAR-PERSIS
2
641
2.49 %
15
2.34 %
581
90.64 %
50
7.80 %
44
6.86 %
1
0.16 %
PAR-RECUR
4
651
2.53 %
30
4.61 %
572
87.86 %
51
7.83 %
49
7.53 %
0
0.00 %
Wiltshire
PAR-CUL-N
1
236
0.92 %
52
22.03 %
173
73.31 %
16
6.78 %
11
4.66 %
0
0.00 %
PAR-RECUR
2
253
0.98 %
3
1.19 %
234
92.49 %
18
7.11 %
15
5.93 %
1
0.40 %
SER-FLEXI
1
50
0.19 %
9
18.00 %
38
76.00 %
3
6.00 %
3
6.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
14
0.05 %
1
7.14 %
12
85.71 %
1
7.14 %
1
7.14 %
0
0.00 %
Bucks
PAR-NEW-IN
4
404
1.57 %
15
3.71 %
367
90.84 %
26
6.44 %
22
5.45 %
0
0.00 %
Cheshire
PAR-CUL-N
2
157
0.61 %
7
4.46 %
142
90.45 %
11
7.01 %
8
5.10 %
0
0.00 %
PAR-NEW-IN
1
73
0.28 %
0
0.00 %
69
94.52 %
6
8.22 %
4
5.48 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
471
1.83 %
18
3.82 %
428
90.87 %
33
7.01 %
25
5.31 %
0
0.00 %
Hampshire
PAR-NEW-IN
3
279
1.08 %
13
4.66 %
238
85.30 %
30
10.75 %
28
10.04 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
1
80
0.31 %
1
1.25 %
71
88.75 %
9
11.25 %
8
10.00 %
0
0.00 %
Northants
PAR-NEW-IN
2
358
1.39 %
25
6.98 %
305
85.20 %
32
8.94 %
28
7.82 %
0
0.00 %
Notts
PAR-NEW-IN
3
447
1.74 %
15
3.36 %
408
91.28 %
30
6.71 %
24
5.37 %
0
0.00 %
Oxon
PAR-CUL-P
1
32
0.12 %
2
6.25 %
17
53.13 %
13
40.63 %
13
40.63 %
0
0.00 %
PAR-NEW-IN
2
171
0.66 %
5
2.92 %
138
80.70 %
33
19.30 %
28
16.37 %
0
0.00 %
PAR-PERSIS
2
647
2.51 %
31
4.79 %
584
90.26 %
39
6.03 %
32
4.95 %
0
0.00 %
PAR-RECUR
3
452
1.76 %
17
3.76 %
405
89.60 %
34
7.52 %
30
6.64 %
0
0.00 %
Warks
PAR-RECUR
1
20
0.08 %
0
0.00 %
18
90.00 %
2
10.00 %
2
10.00 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Lincs
PAR-NEW-IN
3
366
1.42 %
6
1.64 %
338
92.35 %
30
8.20 %
21
5.74 %
1
0.27 %
N Yorks
PAR-NEW-IN
1
6
0.02 %
0
0.00 %
5
83.33 %
2
33.33 %
1
16.67 %
0
0.00 %
England
143
20481
79.58 %
953
4.65 %
17835
87.08 %
1818
8.88 %
1579
7.71 %
114
0.56 %
Wales - High TB
Carms
PAR-IR-SEV
7
89
0.35 %
38
42.70 %
43
48.31 %
8
8.99 %
8
8.99 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
5
0.02 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
66
0.26 %
2
3.03 %
38
57.58 %
28
42.42 %
26
39.39 %
0
0.00 %
PAR-RAPID
9
32
0.12 %
13
40.63 %
18
56.25 %
1
3.13 %
1
3.13 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Ceredigion
PAR-IR-EX
3
5
0.02 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
19
0.07 %
3
15.79 %
16
84.21 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
224
0.87 %
32
14.29 %
176
78.57 %
18
8.04 %
16
7.14 %
0
0.00 %
PAR-RAPID
6
15
0.06 %
5
33.33 %
10
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Gwent
PAR-IR-EX
1
13
0.05 %
9
69.23 %
4
30.77 %
1
7.69 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
32
0.12 %
10
31.25 %
20
62.50 %
3
9.38 %
2
6.25 %
0
0.00 %
PAR-RAPID
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-EX
4
31
0.12 %
18
58.06 %
10
32.26 %
3
9.68 %
3
9.68 %
0
0.00 %
PAR-IR-SEV
14
68
0.26 %
28
41.18 %
38
55.88 %
2
2.94 %
2
2.94 %
0
0.00 %
PAR-PERSIS
7
687
2.67 %
37
5.39 %
600
87.34 %
61
8.88 %
50
7.28 %
0
0.00 %
PAR-RAPID
24
71
0.28 %
22
30.99 %
40
56.34 %
9
12.68 %
9
12.68 %
0
0.00 %
PAR-RECUR
5
70
0.27 %
1
1.43 %
52
74.29 %
23
32.86 %
17
24.29 %
0
0.00 %
SER-FLEXI
5
499
1.94 %
28
5.61 %
438
87.78 %
43
8.62 %
32
6.41 %
1
0.20 %
Powys
PAR-IR-EX
2
17
0.07 %
5
29.41 %
12
70.59 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
7
0.03 %
1
14.29 %
5
71.43 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-PERSIS
1
49
0.19 %
0
0.00 %
44
89.80 %
5
10.20 %
3
6.12 %
2
4.08 %
PAR-RAPID
3
6
0.02 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-RECUR
2
352
1.37 %
8
2.27 %
315
89.49 %
32
9.09 %
27
7.67 %
2
0.57 %
SER-FLEXI
3
41
0.16 %
4
9.76 %
19
46.34 %
18
43.90 %
18
43.90 %
0
0.00 %
S Wales
PAR-IR-SEV
1
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
3
73
0.28 %
5
6.85 %
61
83.56 %
7
9.59 %
6
8.22 %
1
1.37 %
Wales - Intermediate TB
Carms
PAR-OTHER
3
468
1.82 %
36
7.69 %
387
82.69 %
47
10.04 %
32
6.84 %
13
2.78 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
8
0.03 %
6
75.00 %
2
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
16
0.06 %
9
56.25 %
7
43.75 %
0
0.00 %
0
0.00 %
0
0.00 %
Mid Glamorgan
PAR-OTHER
1
8
0.03 %
3
37.50 %
5
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
410
1.59 %
8
1.95 %
351
85.61 %
56
13.66 %
49
11.95 %
2
0.49 %
PAR-POSTM
4
25
0.10 %
0
0.00 %
25
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
6
0.02 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-OTHER
2
124
0.48 %
0
0.00 %
121
97.58 %
4
3.23 %
3
2.42 %
0
0.00 %
PAR-PERSIS
1
28
0.11 %
3
10.71 %
23
82.14 %
3
10.71 %
2
7.14 %
0
0.00 %
PAR-POSTM
2
6
0.02 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
8
363
1.41 %
14
3.86 %
330
90.91 %
22
6.06 %
19
5.23 %
0
0.00 %
PAR-POSTM
3
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
5
284
1.10 %
14
4.93 %
242
85.21 %
31
10.92 %
28
9.86 %
0
0.00 %
PAR-POSTM
7
9
0.03 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
139
0.54 %
6
4.32 %
124
89.21 %
10
7.19 %
9
6.47 %
0
0.00 %
Wales
178
4399
17.09 %
382
8.68 %
3630
82.52 %
439
9.98 %
366
8.32 %
21
0.48 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
2
46
0.18 %
1
2.17 %
43
93.48 %
1
2.17 %
1
2.17 %
1
2.17 %
Wigtown
PAR-NEW-IN
6
811
3.15 %
13
1.60 %
734
90.51 %
74
9.12 %
63
7.77 %
1
0.12 %
Scotland
8
857
3.33 %
14
1.63 %
777
90.67 %
75
8.75 %
64
7.47 %
2
0.23 %
Grand Total
Sum:
329
25737
1349
22242
2332
2009
137
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
7
882
0.31 %
66
7.48 %
774
87.76 %
46
5.22 %
39
4.42 %
3
0.34 %
PAR-CUL-P
7
829
0.30 %
48
5.79 %
721
86.97 %
85
10.25 %
60
7.24 %
0
0.00 %
PAR-HERD-S
3
397
0.14 %
38
9.57 %
344
86.65 %
19
4.79 %
15
3.78 %
0
0.00 %
PAR-OTHER
6
324
0.12 %
34
10.49 %
258
79.63 %
44
13.58 %
31
9.57 %
1
0.31 %
PAR-PERSIS
12
1,679
0.60 %
211
12.57 %
1335
79.51 %
148
8.81 %
124
7.39 %
9
0.54 %
PAR-RECUR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-ANOM
3
26
0.01 %
0
0.00 %
22
84.62 %
3
11.54 %
3
11.54 %
1
3.85 %
Corn & Scilly
PAR-CUL-N
100
9,024
3.22 %
402
4.45 %
7790
86.33 %
889
9.85 %
746
8.27 %
86
0.95 %
PAR-CUL-P
19
3,572
1.27 %
282
7.89 %
3073
86.03 %
245
6.86 %
215
6.02 %
2
0.06 %
PAR-OTHER
6
652
0.23 %
83
12.73 %
514
78.83 %
40
6.13 %
31
4.75 %
24
3.68 %
PAR-PERSIS
10
1,207
0.43 %
41
3.40 %
1112
92.13 %
68
5.63 %
54
4.47 %
0
0.00 %
PAR-RECUR
8
1,153
0.41 %
88
7.63 %
995
86.30 %
79
6.85 %
70
6.07 %
0
0.00 %
SER-FLEXI
4
604
0.22 %
30
4.97 %
552
91.39 %
27
4.47 %
22
3.64 %
0
0.00 %
SER-OTHER
1
7
0.00 %
0
0.00 %
6
85.71 %
3
42.86 %
1
14.29 %
0
0.00 %
Derbyshire
PAR-RECUR
1
515
0.18 %
10
1.94 %
476
92.43 %
30
5.83 %
29
5.63 %
0
0.00 %
Devon
PAR-CUL-N
348
37,178
13.27 %
2282
6.14 %
32670
87.87 %
2532
6.81 %
2078
5.59 %
148
0.40 %
PAR-CUL-P
15
1,555
0.55 %
89
5.72 %
1337
85.98 %
160
10.29 %
125
8.04 %
4
0.26 %
PAR-NEW-IN
12
1,003
0.36 %
63
6.28 %
842
83.95 %
98
9.77 %
95
9.47 %
3
0.30 %
PAR-OTHER
5
365
0.13 %
27
7.40 %
322
88.22 %
20
5.48 %
16
4.38 %
0
0.00 %
PAR-PERSIS
19
3,242
1.16 %
133
4.10 %
2937
90.59 %
225
6.94 %
169
5.21 %
3
0.09 %
PAR-RECUR
20
3,415
1.22 %
101
2.96 %
3086
90.37 %
270
7.91 %
226
6.62 %
2
0.06 %
SER-FLEXI
5
117
0.04 %
13
11.11 %
99
84.62 %
7
5.98 %
5
4.27 %
0
0.00 %
Dorset
PAR-CUL-N
75
9,403
3.36 %
576
6.13 %
8364
88.95 %
585
6.22 %
458
4.87 %
5
0.05 %
PAR-CUL-P
4
581
0.21 %
56
9.64 %
511
87.95 %
18
3.10 %
14
2.41 %
0
0.00 %
PAR-OTHER
3
144
0.05 %
8
5.56 %
131
90.97 %
7
4.86 %
5
3.47 %
0
0.00 %
PAR-PERSIS
2
405
0.14 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gloucs
PAR-CUL-N
85
8,411
3.00 %
402
4.78 %
7344
87.31 %
753
8.95 %
628
7.47 %
37
0.44 %
PAR-CUL-P
2
189
0.07 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
PAR-OTHER
1
66
0.02 %
2
3.03 %
62
93.94 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-PERSIS
10
2,251
0.80 %
180
8.00 %
1916
85.12 %
225
10.00 %
151
6.71 %
4
0.18 %
PAR-RECUR
3
252
0.09 %
5
1.98 %
231
91.67 %
18
7.14 %
14
5.56 %
2
0.79 %
Heref
PAR-CUL-N
15
954
0.34 %
48
5.03 %
801
83.96 %
87
9.12 %
79
8.28 %
26
2.73 %
PAR-CUL-P
1
271
0.10 %
18
6.64 %
243
89.67 %
15
5.54 %
10
3.69 %
0
0.00 %
PAR-OTHER
5
726
0.26 %
30
4.13 %
627
86.36 %
74
10.19 %
69
9.50 %
0
0.00 %
PAR-PERSIS
7
1,049
0.37 %
38
3.62 %
572
54.53 %
452
43.09 %
439
41.85 %
0
0.00 %
PAR-RECUR
15
1,744
0.62 %
112
6.42 %
1549
88.82 %
95
5.45 %
82
4.70 %
1
0.06 %
SER-OTHER
1
52
0.02 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
27
4,557
1.63 %
175
3.84 %
4144
90.94 %
253
5.55 %
220
4.83 %
18
0.39 %
PAR-NEW-IN
2
233
0.08 %
13
5.58 %
212
90.99 %
10
4.29 %
8
3.43 %
0
0.00 %
PAR-OTHER
9
1,050
0.37 %
63
6.00 %
950
90.48 %
42
4.00 %
29
2.76 %
8
0.76 %
PAR-PERSIS
1
242
0.09 %
19
7.85 %
211
87.19 %
15
6.20 %
12
4.96 %
0
0.00 %
PAR-RECUR
19
2,422
0.86 %
117
4.83 %
2187
90.30 %
144
5.95 %
115
4.75 %
3
0.12 %
Somerset excl N
PAR-CUL-N
111
12,085
4.31 %
766
6.34 %
10810
89.45 %
660
5.46 %
494
4.09 %
15
0.12 %
PAR-CUL-P
19
2,956
1.05 %
118
3.99 %
2713
91.78 %
144
4.87 %
112
3.79 %
13
0.44 %
PAR-NEW-IN
2
370
0.13 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.17 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
6
969
0.35 %
35
3.61 %
892
92.05 %
59
6.09 %
40
4.13 %
2
0.21 %
PAR-RECUR
17
2,637
0.94 %
106
4.02 %
2403
91.13 %
164
6.22 %
127
4.82 %
1
0.04 %
SER-FLEXI
3
55
0.02 %
9
16.36 %
42
76.36 %
2
3.64 %
2
3.64 %
2
3.64 %
Staffs
PAR-CUL-N
80
11,512
4.11 %
410
3.56 %
10368
90.06 %
859
7.46 %
732
6.36 %
2
0.02 %
PAR-CUL-P
5
471
0.17 %
42
8.92 %
406
86.20 %
39
8.28 %
23
4.88 %
0
0.00 %
PAR-NEW-IN
3
44
0.02 %
2
4.55 %
36
81.82 %
7
15.91 %
6
13.64 %
0
0.00 %
PAR-OTHER
2
188
0.07 %
5
2.66 %
157
83.51 %
25
13.30 %
26
13.83 %
0
0.00 %
PAR-PERSIS
3
645
0.23 %
15
2.33 %
583
90.39 %
52
8.06 %
46
7.13 %
1
0.16 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
8
1,283
0.46 %
55
4.29 %
1136
88.54 %
104
8.11 %
92
7.17 %
0
0.00 %
Wiltshire
PAR-CUL-N
39
4,014
1.43 %
301
7.50 %
3426
85.35 %
336
8.37 %
278
6.93 %
9
0.22 %
PAR-CUL-P
13
1,096
0.39 %
40
3.65 %
988
90.15 %
62
5.66 %
49
4.47 %
19
1.73 %
PAR-NEW-IN
2
191
0.07 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
11
1,751
0.62 %
76
4.34 %
1573
89.83 %
115
6.57 %
95
5.43 %
7
0.40 %
PAR-RECUR
3
439
0.16 %
11
2.51 %
403
91.80 %
27
6.15 %
23
5.24 %
2
0.46 %
SER-FLEXI
3
222
0.08 %
22
9.91 %
186
83.78 %
4
1.80 %
4
1.80 %
10
4.50 %
Worcs
PAR-CUL-N
2
128
0.05 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
PAR-PERSIS
3
323
0.12 %
45
13.93 %
263
81.42 %
16
4.95 %
15
4.64 %
0
0.00 %
England - Edge
Berks
PAR-CUL-P
2
319
0.11 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
20
1,480
0.53 %
83
5.61 %
1296
87.57 %
112
7.57 %
101
6.82 %
0
0.00 %
PAR-PERSIS
4
247
0.09 %
9
3.64 %
234
94.74 %
7
2.83 %
4
1.62 %
0
0.00 %
PAR-RECUR
1
42
0.01 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
16
1,992
0.71 %
58
2.91 %
1855
93.12 %
96
4.82 %
77
3.87 %
2
0.10 %
PAR-PERSIS
4
379
0.14 %
7
1.85 %
353
93.14 %
22
5.80 %
19
5.01 %
0
0.00 %
Cheshire
PAR-CUL-N
68
10,220
3.65 %
333
3.26 %
9424
92.21 %
615
6.02 %
462
4.52 %
1
0.01 %
PAR-NEW-IN
42
6,320
2.26 %
175
2.77 %
5818
92.06 %
410
6.49 %
319
5.05 %
8
0.13 %
PAR-PERSIS
4
348
0.12 %
12
3.45 %
316
90.80 %
22
6.32 %
19
5.46 %
1
0.29 %
Derbyshire
PAR-CUL-N
7
985
0.35 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
129
13,794
4.92 %
573
4.15 %
11990
86.92 %
1380
10.00 %
1163
8.43 %
68
0.49 %
PAR-RECUR
2
309
0.11 %
8
2.59 %
247
79.94 %
63
20.39 %
54
17.48 %
0
0.00 %
E Sussex
PAR-NEW-IN
10
806
0.29 %
82
10.17 %
660
81.89 %
54
6.70 %
47
5.83 %
17
2.11 %
Hampshire
PAR-CUL-N
3
220
0.08 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
19
2,905
1.04 %
90
3.10 %
2604
89.64 %
237
8.16 %
209
7.19 %
2
0.07 %
PAR-PERSIS
1
76
0.03 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
40
4,599
1.64 %
201
4.37 %
4148
90.19 %
311
6.76 %
249
5.41 %
1
0.02 %
PAR-PERSIS
3
910
0.32 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
45
2,939
1.05 %
111
3.78 %
2652
90.23 %
240
8.17 %
175
5.95 %
1
0.03 %
PAR-PERSIS
3
184
0.07 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
10
1,219
0.43 %
46
3.77 %
1099
90.16 %
102
8.37 %
73
5.99 %
1
0.08 %
Oxon
PAR-CUL-P
2
111
0.04 %
6
5.41 %
88
79.28 %
17
15.32 %
17
15.32 %
0
0.00 %
PAR-NEW-IN
72
5,384
1.92 %
209
3.88 %
4812
89.38 %
432
8.02 %
359
6.67 %
4
0.07 %
PAR-PERSIS
14
2,732
0.97 %
120
4.39 %
2486
91.00 %
153
5.60 %
126
4.61 %
0
0.00 %
PAR-RECUR
3
452
0.16 %
17
3.76 %
405
89.60 %
34
7.52 %
30
6.64 %
0
0.00 %
Warks
PAR-NEW-IN
60
4,463
1.59 %
164
3.67 %
4017
90.01 %
314
7.04 %
272
6.09 %
10
0.22 %
PAR-PERSIS
3
163
0.06 %
10
6.13 %
141
86.50 %
13
7.98 %
12
7.36 %
0
0.00 %
PAR-RECUR
3
148
0.05 %
1
0.68 %
77
52.03 %
7
4.73 %
6
4.05 %
64
43.24 %
England - LRA
Beds
PAR-NEW-IN
7
926
0.33 %
34
3.67 %
817
88.23 %
93
10.04 %
69
7.45 %
6
0.65 %
Cleveland
PAR-NEW-IN
2
194
0.07 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
19
2,798
1.00 %
70
2.50 %
2463
88.03 %
293
10.47 %
254
9.08 %
11
0.39 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.03 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Essex
PAR-NEW-IN
3
247
0.09 %
14
5.67 %
203
82.19 %
19
7.69 %
14
5.67 %
16
6.48 %
Gr Manchester
PAR-NEW-IN
8
578
0.21 %
20
3.46 %
535
92.56 %
29
5.02 %
23
3.98 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.04 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Humberside
PAR-NEW-IN
1
118
0.04 %
4
3.39 %
109
92.37 %
6
5.08 %
5
4.24 %
0
0.00 %
Lancashire
PAR-NEW-IN
8
803
0.29 %
24
2.99 %
734
91.41 %
45
5.60 %
41
5.11 %
4
0.50 %
Lincs
PAR-NEW-IN
21
1,821
0.65 %
57
3.13 %
1629
89.46 %
153
8.40 %
128
7.03 %
7
0.38 %
N Yorks
PAR-NEW-IN
15
532
0.19 %
27
5.08 %
390
73.31 %
123
23.12 %
113
21.24 %
2
0.38 %
Suffolk
PAR-NEW-IN
4
328
0.12 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.07 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
1924
217128
77.48 %
10811
4.98 %
192098
88.47 %
16321
7.52 %
13490
6.21 %
729
0.34 %
Wales - High TB
Carms
PAR-IR-EX
9
48
0.02 %
21
43.75 %
24
50.00 %
2
4.17 %
2
4.17 %
1
2.08 %
PAR-IR-SEV
56
479
0.17 %
128
26.72 %
322
67.22 %
31
6.47 %
29
6.05 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
22
605
0.22 %
104
17.19 %
471
77.85 %
37
6.12 %
28
4.63 %
2
0.33 %
PAR-PERSIS
37
4,205
1.50 %
157
3.73 %
3883
92.34 %
219
5.21 %
161
3.83 %
4
0.10 %
PAR-RAPID
78
236
0.08 %
73
30.93 %
157
66.53 %
6
2.54 %
6
2.54 %
0
0.00 %
PAR-RECUR
8
581
0.21 %
27
4.65 %
530
91.22 %
33
5.68 %
22
3.79 %
2
0.34 %
SER-FLEXI
10
256
0.09 %
27
10.55 %
220
85.94 %
12
4.69 %
8
3.13 %
1
0.39 %
Ceredigion
PAR-IR-EX
10
31
0.01 %
7
22.58 %
24
77.42 %
1
3.23 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
45
317
0.11 %
78
24.61 %
223
70.35 %
20
6.31 %
14
4.42 %
2
0.63 %
PAR-OTHER
3
64
0.02 %
6
9.38 %
53
82.81 %
7
10.94 %
5
7.81 %
0
0.00 %
PAR-PERSIS
17
1,413
0.50 %
104
7.36 %
1225
86.69 %
96
6.79 %
71
5.02 %
13
0.92 %
PAR-RAPID
44
121
0.04 %
31
25.62 %
85
70.25 %
8
6.61 %
5
4.13 %
0
0.00 %
PAR-RECUR
2
7
0.00 %
1
14.29 %
4
57.14 %
3
42.86 %
2
28.57 %
0
0.00 %
SER-FLEXI
6
215
0.08 %
18
8.37 %
190
88.37 %
8
3.72 %
6
2.79 %
1
0.47 %
Gwent
PAR-IR-EX
3
29
0.01 %
11
37.93 %
18
62.07 %
2
6.90 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
9
81
0.03 %
11
13.58 %
65
80.25 %
6
7.41 %
5
6.17 %
0
0.00 %
PAR-OTHER
7
389
0.14 %
18
4.63 %
349
89.72 %
33
8.48 %
21
5.40 %
1
0.26 %
PAR-PERSIS
12
485
0.17 %
34
7.01 %
421
86.80 %
31
6.39 %
28
5.77 %
2
0.41 %
PAR-RAPID
9
27
0.01 %
6
22.22 %
21
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
116
0.04 %
10
8.62 %
100
86.21 %
6
5.17 %
6
5.17 %
0
0.00 %
SER-FLEXI
2
28
0.01 %
5
17.86 %
19
67.86 %
4
14.29 %
4
14.29 %
0
0.00 %
Pembs
PAR-HERD-S
4
19
0.01 %
2
10.53 %
12
63.16 %
6
31.58 %
5
26.32 %
0
0.00 %
PAR-IR-EX
33
188
0.07 %
56
29.79 %
122
64.89 %
10
5.32 %
9
4.79 %
1
0.53 %
PAR-IR-SEV
168
1,304
0.47 %
313
24.00 %
941
72.16 %
60
4.60 %
49
3.76 %
1
0.08 %
PAR-OTHER
35
1,310
0.47 %
82
6.26 %
1170
89.31 %
66
5.04 %
55
4.20 %
3
0.23 %
PAR-PERSIS
108
6,845
2.44 %
292
4.27 %
6239
91.15 %
402
5.87 %
304
4.44 %
10
0.15 %
PAR-RAPID
179
498
0.18 %
154
30.92 %
317
63.65 %
34
6.83 %
27
5.42 %
0
0.00 %
PAR-RECUR
34
4,799
1.71 %
183
3.81 %
4436
92.44 %
220
4.58 %
166
3.46 %
14
0.29 %
SER-FLEXI
58
2,305
0.82 %
176
7.64 %
2041
88.55 %
117
5.08 %
80
3.47 %
8
0.35 %
Powys
PAR-HERD-S
1
10
0.00 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-EX
16
99
0.04 %
26
26.26 %
69
69.70 %
6
6.06 %
4
4.04 %
0
0.00 %
PAR-IR-SEV
24
132
0.05 %
28
21.21 %
99
75.00 %
7
5.30 %
5
3.79 %
0
0.00 %
PAR-NEW-IN
1
239
0.09 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
17
939
0.34 %
41
4.37 %
840
89.46 %
65
6.92 %
49
5.22 %
9
0.96 %
PAR-PERSIS
41
2,059
0.73 %
84
4.08 %
1749
84.94 %
237
11.51 %
216
10.49 %
10
0.49 %
PAR-RAPID
31
60
0.02 %
10
16.67 %
47
78.33 %
4
6.67 %
3
5.00 %
0
0.00 %
PAR-RECUR
14
1,786
0.64 %
65
3.64 %
1627
91.10 %
107
5.99 %
87
4.87 %
7
0.39 %
SER-FLEXI
13
249
0.09 %
24
9.64 %
194
77.91 %
35
14.06 %
31
12.45 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.37 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
3
25
0.01 %
7
28.00 %
18
72.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
5
140
0.05 %
5
3.57 %
122
87.14 %
14
10.00 %
12
8.57 %
1
0.71 %
PAR-PERSIS
2
136
0.05 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.00 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
13
1,271
0.45 %
107
8.42 %
1085
85.37 %
94
7.40 %
63
4.96 %
16
1.26 %
PAR-PERSIS
6
435
0.16 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
10
19
0.01 %
5
26.32 %
14
73.68 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.08 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.03 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
8
0.00 %
6
75.00 %
2
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
18
0.01 %
2
11.11 %
16
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
106
0.04 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.03 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
4
21
0.01 %
12
57.14 %
9
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.05 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.05 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.17 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Mid Glamorgan
PAR-OTHER
1
8
0.00 %
3
37.50 %
5
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
4
10
0.00 %
4
40.00 %
6
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
37
242
0.09 %
38
15.70 %
194
80.17 %
12
4.96 %
10
4.13 %
0
0.00 %
PAR-NEW-IN
125
10,511
3.75 %
339
3.23 %
9558
90.93 %
738
7.02 %
601
5.72 %
13
0.12 %
PAR-OTHER
5
106
0.04 %
4
3.77 %
95
89.62 %
10
9.43 %
7
6.60 %
0
0.00 %
PAR-PERSIS
26
2,113
0.75 %
56
2.65 %
1955
92.52 %
139
6.58 %
102
4.83 %
0
0.00 %
PAR-POSTM
33
143
0.05 %
8
5.59 %
128
89.51 %
8
5.59 %
7
4.90 %
0
0.00 %
PAR-RAPID
22
75
0.03 %
16
21.33 %
55
73.33 %
5
6.67 %
4
5.33 %
0
0.00 %
PAR-RECUR
5
267
0.10 %
24
8.99 %
232
86.89 %
13
4.87 %
11
4.12 %
0
0.00 %
SER-FLEXI
23
830
0.30 %
49
5.90 %
752
90.60 %
42
5.06 %
29
3.49 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
20
1,884
0.67 %
53
2.81 %
xx-xxxx-xxxx
89.28 %
163
8.65 %
146
7.75 %
3
0.16 %
PAR-OTHER
2
124
0.04 %
0
0.00 %
121
97.58 %
4
3.23 %
3
2.42 %
0
0.00 %
PAR-PERSIS
2
42
0.01 %
5
11.90 %
31
73.81 %
7
16.67 %
6
14.29 %
0
0.00 %
PAR-POSTM
9
24
0.01 %
4
16.67 %
19
79.17 %
2
8.33 %
1
4.17 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
196
0.07 %
8
4.08 %
169
86.22 %
23
11.73 %
18
9.18 %
1
0.51 %
S Glamorgan
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
9
0.00 %
5
55.56 %
4
44.44 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
221
0.08 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-IR-SEV
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
126
0.04 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
57
0.02 %
1
1.75 %
55
96.49 %
1
1.75 %
1
1.75 %
0
0.00 %
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
51
2,372
0.85 %
69
2.91 %
2135
90.01 %
212
8.94 %
164
6.91 %
4
0.17 %
PAR-OTHER
2
51
0.02 %
0
0.00 %
50
98.04 %
2
3.92 %
1
1.96 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-POSTM
16
37
0.01 %
0
0.00 %
34
91.89 %
4
10.81 %
3
8.11 %
0
0.00 %
PAR-RAPID
4
5
0.00 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
65
0.02 %
7
10.77 %
53
81.54 %
6
9.23 %
5
7.69 %
0
0.00 %
NE Wales
PAR-HERD-S
4
111
0.04 %
9
8.11 %
99
89.19 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-IR-EX
2
7
0.00 %
1
14.29 %
5
71.43 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-NEW-IN
48
3,284
1.17 %
153
4.66 %
2878
87.64 %
291
8.86 %
246
7.49 %
7
0.21 %
PAR-OTHER
4
152
0.05 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.02 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
PAR-POSTM
38
77
0.03 %
3
3.90 %
72
93.51 %
6
7.79 %
2
2.60 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
10
953
0.34 %
59
6.19 %
845
88.67 %
56
5.88 %
49
5.14 %
0
0.00 %
Wales
1768
61103
21.80 %
3687
6.03 %
54062
88.48 %
4027
6.59 %
3201
5.24 %
153
0.25 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.07 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
9
892
0.32 %
114
12.78 %
709
79.48 %
66
7.40 %
59
6.61 %
10
1.12 %
PAR-PERSIS
1
108
0.04 %
7
6.48 %
97
89.81 %
7
6.48 %
4
3.70 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.00 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Wigtown
PAR-NEW-IN
6
811
0.29 %
13
1.60 %
734
90.51 %
74
9.12 %
63
7.77 %
1
0.12 %
Scotland
22
2023
0.72 %
150
7.41 %
1724
85.22 %
161
7.96 %
137
6.77 %
12
0.59 %
Grand Total
Sum:
xx-xxxx-xxxx
14648
247884
20509
xx-xxxx-xxxx
894
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol November 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
141
20420
99.7%
942
4.6%
17795
87.1%
1808
8.9%
1569
7.7%
114
0.6%
Serial/Extended
2
61
0.3%
11
18.0%
40
65.6%
10
16.4%
10
16.4%
0
0.0 %
Total
143
20481
100.0%
953
4.7%
17835
87.1%
1818
8.9%
1579
7.7%
114
0.6%
Scotland
Parallel
8
857
100.0%
14
1.6%
777
90.7%
75
8.8%
64
7.5%
2
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
8
857
100.0%
14
1.6%
777
90.7%
75
8.8%
64
7.5%
2
0.2%
Wales
Parallel
167
3717
84.5%
344
9.3%
3048
82.0%
366
9.8%
305
8.2%
20
0.5%
Serial/Extended
11
682
15.5%
38
5.6%
582
85.3%
73
10.7%
61
8.9%
1
0.1%
Total
178
4399
100.0%
382
8.7%
3630
82.5%
439
10.0%
366
8.3%
21
0.5%
GB
Parallel
316
24994
97.1%
1300
5.2%
21620
86.5%
2249
9.0%
1938
7.8%
136
0.5%
Serial/Extended
13
743
2.9%
49
6.6%
622
83.7%
83
11.2%
71
9.6%
1
0.1%
Total
329
25737
100.0%
1349
5.2%
22242
86.4%
2332
9.1%
2009
7.8%
137
0.5%
143.00
178.00
8.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.5% 10737
5.0% 191140
88.5%
16274
7.5%
13452
6.2%
716
0.3%
Serial/Extended
20
1083
0.5%
74
6.8%
958
88.5%
47
4.3%
38
3.5%
13
1.2%
Total
xx-xxxx-xxxx
100.0% 10811
5.0% 192098
88.5%
16321
7.5%
13490
6.2%
729
0.3%
Scotland
Parallel
21
2019
99.8%
150
7.4%
1721
85.2%
161
8.0%
137
6.8%
11
0.5%
Serial/Extended
1
4
0.2%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
22
2023
100.0%
150
7.4%
1724
85.2%
161
8.0%
137
6.8%
12
0.6%
Wales
Parallel
1635
55969
91.6%
3294
5.9%
49549
88.5%
3738
6.7%
2984
5.3%
142
0.3%
Serial/Extended
133
5134
8.4%
393
7.7%
4513
87.9%
289
5.6%
217
4.2%
11
0.2%
Total
1768
61103
100.0%
3687
6.0%
54062
88.5%
4027
6.6%
3201
5.2%
153
0.3%
GB
Parallel
xx-xxxx-xxxx
97.8% 14181
5.2% 242410
88.5%
20173
7.4%
16573
6.0%
869
0.3%
Serial/Extended
154
6221
2.2%
467
7.5%
5474
88.0%
336
5.4%
255
4.1%
25
0.4%
Total
xx-xxxx-xxxx
100.0% 14648
5.2% 247884
88.4%
20509
7.3%
xx-xxxx-xxxx
6.0%
894
0.3%
#######
#######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
743
2.9%
49
6.6%
622
83.7%
83
11.2 %
71
9.6 %
1
0.1 %
Parallel - Herd or Group Slaughter
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - IRs in Exit Strategy for persistent her
15
81
0.3%
41
50.6%
37
45.7%
4
4.9 %
3
3.7 %
0
Parallel New Infection
54
4819
18.7%
162
3.4%
4293
89.1%
427
8.9 %
359
7.4 %
5
0.1 %
Parallel - new infection cull area
24
2902
11.3%
203
7.0%
2461
84.8%
199
6.9 %
165
5.7 %
73
2.5 %
Parallel - Other
22
1553
6.0%
141
9.1%
1272
81.9%
122
7.9 %
102
6.6 %
38
2.4 %
Parallel Persistent Infection
31
4240
16.5%
222
5.2%
3371
79.5%
704
16.6 %
643
15.2 %
4
0.1 %
Parallel - persistent infection cull area
10
2425
9.4%
114
4.7%
2222
91.6%
118
4.9 %
79
3.3 %
10
0.4 %
Parallel – post-mvt test coupled with IDEXX an
16
46
0.2%
2
4.3%
44
95.7%
0
0
0
Parallel - Rapid Testing Two-times IRs
48
152
0.6%
54
35.5%
87
57.2%
11
7.2 %
11
7.2 %
0
Parallel - Recurrent breakdowns
60
8549
33.2%
279
3.3%
7701
90.1%
650
7.6 %
563
6.6 %
6
0.1 %
Parallel - severe IRs - in persistent herds
35
225
0.9%
82
36.4%
130
57.8%
14
6.2 %
13
5.8 %
0
Total
329
25737
100.0%
1349
5.2%
22242
86.4%
2332
9.1 %
2009
7.8 %
137
0.5 %
Table 4a. Summaryof IFN Gamma results by submission reason (November 2021)
England - HRA
Extended Flexible IFN-GAMMA test
2
61
0.2%
11
18.0%
40
65.6%
10
16.4 %
10
16.4 %
0
Parallel New Infection
2
233
0.9%
13
5.6%
212
91.0%
10
4.3 %
8
3.4 %
0
Parallel - new infection cull area
22
2745
10.7%
196
7.1%
2319
84.5%
188
6.8 %
157
5.7 %
73
2.7 %
Parallel - Other
12
875
3.4%
95
10.9%
695
79.4%
64
7.3 %
61
7.0 %
24
2.7 %
Parallel Persistent Infection
11
2539
9.9%
117
4.6%
1906
75.1%
550
21.7 %
514
20.2 %
2
0.1 %
Parallel - persistent infection cull area
9
2393
9.3%
112
4.7%
2205
92.1%
105
4.4 %
66
2.8 %
10
0.4 %
Parallel - Recurrent breakdowns
49
7655
29.7%
253
3.3%
6911
90.3%
559
7.3 %
487
6.4 %
4
0.1 %
England - HRA
107
16501
64.1%
797
4.8%
14288
86.6%
1486
9.0 %
1303
7.9 %
113
0.7 %
England - Edge
Parallel New Infection
22
2297
8.9%
93
4.0%
2036
88.6%
200
8.7 %
168
7.3 %
0
Parallel - new infection cull area
2
157
0.6%
7
4.5%
142
90.4%
11
7.0 %
8
5.1 %
0
Parallel Persistent Infection
2
647
2.5%
31
4.8%
584
90.3%
39
6.0 %
32
4.9 %
0
Parallel - persistent infection cull area
1
32
0.1%
2
6.3%
17
53.1%
13
40.6 %
13
40.6 %
0
Parallel - Recurrent breakdowns
4
472
1.8%
17
3.6%
423
89.6%
36
7.6 %
32
6.8 %
0
England - Edge
31
3605
14.0%
150
4.2%
3202
88.8%
299
8.3 %
253
7.0 %
0
England - LRA
Parallel New Infection
5
375
1.5%
6
1.6%
345
92.0%
33
8.8 %
23
6.1 %
1
0.3 %
England - LRA
5
375
1.5%
6
1.6%
345
92.0%
33
8.8 %
23
6.1 %
1
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
10
543
2.1%
32
5.9%
458
84.3%
63
11.6 %
52
9.6 %
1
0.2 %
Parallel - Herd or Group Slaughter
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - IRs in Exit Strategy for persistent her
10
66
0.3%
34
51.5%
29
43.9%
4
6.1 %
3
4.5 %
0
Parallel - Other
4
78
0.3%
7
9.0%
64
82.1%
7
9.0 %
6
7.7 %
1
1.3 %
Parallel Persistent Infection
17
1026
4.0%
71
6.9%
858
83.6%
112
10.9 %
95
9.3 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
43
127
0.5%
41
32.3%
75
59.1%
11
8.7 %
11
8.7 %
0
Parallel - Recurrent breakdowns
7
422
1.6%
9
2.1%
367
87.0%
55
13.0 %
44
10.4 %
2
0.5 %
Parallel - severe IRs - in persistent herds
31
218
0.8%
82
37.6%
123
56.4%
14
6.4 %
13
6.0 %
0
Wales - High TB
123
2482
9.6%
276
11.1%
1976
79.6%
266
10.7 %
224
9.0 %
6
0.2 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
3
13
0.1%
6
46.2%
7
53.8%
0
0
0
Parallel New Infection
4
410
1.6%
8
2.0%
351
85.6%
56
13.7 %
49
12.0 %
2
0.5 %
Parallel - Other
6
600
2.3%
39
6.5%
513
85.5%
51
8.5 %
35
5.8 %
13
2.2 %
Parallel Persistent Infection
1
28
0.1%
3
10.7%
23
82.1%
3
10.7 %
2
7.1 %
0
Parallel – post-mvt test coupled with IDEXX an
6
31
0.1%
2
6.5%
29
93.5%
0
0
0
Parallel - Rapid Testing Two-times IRs
4
23
0.1%
12
52.2%
11
47.8%
0
0
0
Parallel - severe IRs - in persistent herds
4
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Wales - Intermediate TB
28
1112
4.3%
70
6.3%
941
84.6%
110
9.9 %
86
7.7 %
15
1.3 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
139
0.5%
6
4.3%
124
89.2%
10
7.2 %
9
6.5 %
0
Parallel - IRs in Exit Strategy for persistent her
2
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel New Infection
13
647
2.5%
28
4.3%
572
88.4%
53
8.2 %
47
7.3 %
0
Parallel – post-mvt test coupled with IDEXX an
10
15
0.1%
0
0.0 %
15
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales - Low TB
27
805
3.1%
36
4.5%
713
88.6%
63
7.8 %
56
7.0 %
0
Scotland - Scotland
Parallel New Infection
8
857
3.3%
14
1.6%
777
90.7%
75
8.8 %
64
7.5 %
2
0.2 %
Scotland - Scotland
8
857
3.3%
14
1.6%
777
90.7%
75
8.8 %
64
7.5 %
2
0.2 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
148
6132
2.2%
467
7.6%
5392
87.9%
329
5.4 %
250
4.1 %
23
0.4 %
Parallel - Herd or Group Slaughter
22
1633
0.6%
98
6.0%
1465
89.7%
78
4.8 %
65
4.0 %
5
0.3 %
Parallel - IRs in Exit Strategy for persistent herds
80
425
0.2%
133
31.3%
274
64.5%
22
5.2 %
16
3.8 %
2
0.5 %
Parallel New Infection
852
77294
27.6%
3009
3.9%
68788
89.0%
6330
8.2 %
5283
6.8 %
214
0.3 %
Parallel - new infection cull area
969 109608
39.1%
5842
5.3%
97065
88.6%
7754
7.1 %
6347
5.8 %
354
0.3 %
Parallel - Other
159
9248
3.3%
654
7.1%
8005
86.6%
639
6.9 %
522
5.6 %
67
0.7 %
Parallel Persistent Infection
376
36759
13.1%
1773
4.8%
32594
88.7%
2858
7.8 %
2324
6.3 %
68
0.2 %
Parallel - persistent infection cull area
89
11950
4.3%
737
6.2%
10520
88.0%
803
6.7 %
637
5.3 %
56
0.5 %
Parallel – post-mvt test coupled with IDEXX anti
96
281
0.1%
15
5.3%
253
90.0%
20
7.1 %
13
4.6 %
0
Parallel - Rapid Testing Two-times IRs
389
1081
0.4%
315
29.1%
721
66.7%
58
5.4 %
45
4.2 %
0
Parallel - Recurrent breakdowns
182
23143
8.3%
999
4.3%
20835
90.0%
1475
6.4 %
1209
5.2 %
100
0.4 %
Parallel - severe IRs - in persistent herds
346
2611
0.9%
606
23.2%
1890
72.4%
136
5.2 %
112
4.3 %
3
0.1 %
Serial - Other
2
59
0.0%
0
0.0 %
57
96.6%
4
6.8 %
2
3.4 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
xx-xxxx-xxxx
100.0%
14648
5.2%
247884
88.4%
20509
7.3 %
xx-xxxx-xxxx
6.0 %
894
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
15
998
0.4%
74
7.4%
879
88.1%
40
4.0 %
33
3.3 %
12
1.2 %
Parallel - Herd or Group Slaughter
3
397
0.1%
38
9.6%
344
86.6%
19
4.8 %
15
3.8 %
0
Parallel New Infection
21
1841
0.7%
144
7.8%
1559
84.7%
143
7.8 %
135
7.3 %
3
0.2 %
Parallel - new infection cull area
889
98148
35.0%
5440
5.5%
86603
88.2%
7003
7.1 %
5754
5.9 %
351
0.4 %
Parallel - Other
39
3982
1.4%
274
6.9%
3410
85.6%
289
7.3 %
265
6.7 %
33
0.8 %
Parallel Persistent Infection
84
13763
4.9%
801
5.8%
11776
85.6%
1393
10.1 %
1160
8.4 %
26
0.2 %
Parallel - persistent infection cull area
85
11520
4.1%
728
6.3%
10140
88.0%
776
6.7 %
614
5.3 %
38
0.3 %
Parallel - Recurrent breakdowns
95
13861
4.9%
605
4.4%
12467
89.9%
931
6.7 %
778
5.6 %
11
0.1 %
Serial - Other
2
59
0.0%
0
0.0 %
57
96.6%
4
6.8 %
2
3.4 %
0
England - Edge
Parallel New Infection
463
45901
16.4%
1792
3.9%
40951
89.2%
3688
8.0 %
3044
6.6 %
114
0.2 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - new infection cull area
79
11442
4.1%
402
3.5%
10456
91.4%
741
6.5 %
583
5.1 %
1
0.0 %
Parallel Persistent Infection
36
5039
1.8%
189
3.8%
4603
91.3%
292
5.8 %
245
4.9 %
2
0.0 %
Parallel - persistent infection cull area
4
430
0.2%
9
2.1%
380
88.4%
27
6.3 %
23
5.3 %
18
4.2 %
Parallel - Recurrent breakdowns
9
951
0.3%
27
2.8%
767
80.7%
107
11.3 %
93
9.8 %
64
6.7 %
England - LRA
Parallel New Infection
96
8752
3.1%
288
3.3%
7678
87.7%
855
9.8 %
733
8.4 %
53
0.6 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
xx-xxxx-xxxx
77.5%
10811
5.0%
192098
88.5%
16321
7.5 %
13490
6.2 %
729
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
89
3053
1.1%
250
8.2%
2664
87.3%
176
5.8 %
129
4.2 %
10
0.3 %
Parallel - Herd or Group Slaughter
10
1059
0.4%
49
4.6%
960
90.7%
52
4.9 %
45
4.2 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
71
395
0.1%
121
30.6%
257
65.1%
21
5.3 %
15
3.8 %
2
0.5 %
Parallel New Infection
2
245
0.1%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
89
3447
1.2%
256
7.4%
3005
87.2%
222
6.4 %
170
4.9 %
16
0.5 %
Parallel Persistent Infection
217
15143
5.4%
673
4.4%
13646
90.1%
990
6.5 %
785
5.2 %
39
0.3 %
Parallel - Rapid Testing Two-times IRs
344
950
0.3%
275
28.9%
634
66.7%
53
5.6 %
41
4.3 %
0
Parallel - Recurrent breakdowns
61
7289
2.6%
286
3.9%
6697
91.9%
369
5.1 %
283
3.9 %
23
0.3 %
Parallel - severe IRs - in persistent herds
305
2338
0.8%
565
24.2%
1668
71.3%
124
5.3 %
102
4.4 %
3
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
30
1063
0.4%
77
7.2%
951
89.5%
51
4.8 %
34
3.2 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
6
22
0.0%
10
45.5%
12
54.5%
0
0
0
Parallel New Infection
151
12988
4.6%
414
3.2%
11745
90.4%
967
7.4 %
808
6.2 %
21
0.2 %
Parallel - Other
25
1616
0.6%
120
7.4%
1400
86.6%
118
7.3 %
79
4.9 %
17
1.1 %
Parallel Persistent Infection
36
2663
1.0%
101
3.8%
2432
91.3%
176
6.6 %
130
4.9 %
0
Parallel – post-mvt test coupled with IDEXX ant
42
167
0.1%
12
7.2%
147
88.0%
10
6.0 %
8
4.8 %
0
Parallel - Rapid Testing Two-times IRs
40
125
0.0%
38
30.4%
83
66.4%
5
4.0 %
4
3.2 %
0
Parallel - Recurrent breakdowns
17
1042
0.4%
81
7.8%
904
86.8%
68
6.5 %
55
5.3 %
2
0.2 %
Parallel - severe IRs - in persistent herds
40
272
0.1%
41
15.1%
221
81.3%
12
4.4 %
10
3.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
14
1018
0.4%
66
6.5%
898
88.2%
62
6.1 %
54
5.3 %
0
Parallel - Herd or Group Slaughter
7
168
0.1%
10
6.0%
154
91.7%
6
3.6 %
4
2.4 %
0
Parallel - IRs in Exit Strategy for persistent herd
3
8
0.0%
2
25.0%
5
62.5%
1
12.5 %
1
12.5 %
0
Parallel New Infection
99
5656
2.0%
222
3.9%
5013
88.6%
503
8.9 %
410
7.2 %
11
0.2 %
Parallel - Other
6
203
0.1%
4
2.0%
190
93.6%
10
4.9 %
8
3.9 %
1
0.5 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %
Parallel – post-mvt test coupled with IDEXX ant
54
114
0.0%
3
2.6%
106
93.0%
10
8.8 %
5
4.4 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - Rapid Testing Two-times IRs
5
6
0.0%
2
33.3%
4
66.7%
0
0
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
1768
61103
21.8%
3687
6.0%
54062
88.5%
4027
6.6 %
3201
5.2 %
153
0.3 %
Scotland - Scotland
Parallel New Infection
20
1911
0.7%
143
7.5%
1624
85.0%
154
8.1 %
133
7.0 %
11
0.6 %
Parallel Persistent Infection
1
108
0.0%
7
6.5%
97
89.8%
7
6.5 %
4
3.7 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
22
2023
0.7%
150
7.4%
1724
85.2%
161
8.0 %
137
6.8 %
12
0.6 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
0
84
5
12
March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
4
0
9
0
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
4
0
4
0
1083
5
92
0
1
July
298
24841
164
1077
30
0
0
0
0
61
4
0
4
0
936
0
40
0
2
August
312
17920
167
912
3
26
0
0
0
0
35
0
0
7
2
755
4
78
0
2
September
274
15805
130
890
13
0
0
0
0
95
0
0
5
3
743
9
14
0
7
October
276
19790
140
814
25
0
0
0
0
45
0
18
3
0
687
36
0
0
0
November
307
24479
180
1247
326
519
0
0
0
0
18
0
3
0
0
196
5
0
0
0
Totals (last
12 months)
4016
303142
2324
15774
336
884
0
0
0
0
984
29
21
88
5
12371
60
776
10
25
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2074
236274
1365
11760
660
0
0
0
0
859
13
10
48
2
9694
3
78
0
7
Scotland
27
2493
19
180
12
0
0
0
0
47
1
0
6
0
41
7
51
3
12
Wales
1915
64375
940
3834
212
0
0
0
0
78
15
11
34
3
2636
50
647
7
6
Totals
4016
303142
2324
15774
884
0
0
0
0
984
29
21
88
5
12371
60
776
10
25
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
W Glamorgan
S Wales
Somerset excl N
Powys
Oxon
Notts
NE Wales
Lincs
Heref
Gwynedd
Gr Manchester
Dorset
Derbyshire
Cheshire
Carms
Berks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during November 2021 (% submission per
County)
County
%Resampled %Retested
Avon
2.91 %
8.73 %
Ayrshire
2.17 %
2.17 %
Berks
7.14 %
7.14 %
Bucks
5.45 %
6.44 %
Carms
10.26 %
12.82 %
Ceredigion
5.90 %
6.60 %
Cheshire
5.22 %
7.39 %
Corn & Scilly
6.93 %
7.91 %
Derbyshire
5.48 %
6.39 %
Devon
7.34 %
8.43 %
Dorset
3.54 %
4.13 %
Gloucs
4.71 %
6.59 %
Gr Manchester
33.33 %
33.33 %
Gwent
4.17 %
8.33 %
Gwynedd
5.11 %
5.91 %
Hampshire
10.04 %
10.75 %
Heref
24.65 %
25.90 %
Leics & Rut
10.00 %
11.25 %
Lincs
5.74 %
8.20 %
Mid Glamorgan
NE Wales
9.71 %
10.95 %
Northants
7.82 %
8.94 %
Notts
5.37 %
6.71 %
N Yorks
16.67 %
33.33 %
Oxon
7.91 %
9.14 %
Pembs
7.91 %
9.87 %
Powys
8.73 %
10.16 %
Shropshire
5.45 %
6.45 %
Somerset excl
4.05 %
5.05 %
Staffs
7.26 %
8.06 %
S Wales
Warks
10.00 %
10.00 %
W Glamorgan
8.22 %
9.59 %
Wigtown
7.77 %
9.12 %
Wiltshire
5.38 %
6.86 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20320
953
4.7%
20481
953
4.7%
Wales
4311
382
8.9%
4399
382
8.7%
Scotland
807
14
1.7%
857
14
1.6%
GB
25438
1349
5.3%
25737
1349
5.2%
Table 6a (i): Number of animals and submissions tested in November 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
201858
10808
5.4%
217128
10811
5.0%
Wales
56823
3686
6.5%
61103
3687
6.0%
Scotland
1386
150
10.8%
2023
150
7.4%
GB
260057
14644
5.6%
280254
14648
5.2%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18784
976
5.2%
19084
976
5.1%
Wales
6015
359
6.0%
6221
359
5.8%
Scotland
1270
24
1.9%
1279
24
1.9%
GB
26069
1359
5.2%
26584
1359
5.1%
Table 6a (ii): Number of animals and submissions tested in November 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
191568
7851
4.1%
201915
7851
3.9%
Wales
48393
2923
6.0%
52577
2924
5.6%
Scotland
4905
131
2.7%
5809
131
2.3%
GB
244864
10905
4.5%
260301
10906
4.2%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SE
1
56
7
12.50 %
PRV-SP
1
1
0
0
Worcestershire
PRV-SP
2
3
0
0
Total
4
60
7
11.67 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples November 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
2
2
0
0
Derbyshire
PRV-SP
2
2
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
2
3
0
0
Dorset
PRV-SE
1
18
5
27.78 %
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
7
382
18
4.71 %
PRV-SP
4
6
0
0
Staffordshire
PRV-SP
2
2
0
0
Wiltshire
PRV-SE
1
10
0
0
PRV-SP
1
3
0
0
Worcestershire
PRV-SE
1
1
0
0
PRV-SP
4
6
0
0
Total
35
515
27
5.24 %
Isle of Man
SER-IOM
7
8
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
4
56
12
21.4%
Ceredigion
BOV-IR-C
4
45
4
8.9%
BOV-IR-EX
1
11
2
18.2%
North East Wales
BOV-IR-C
2
8
4
50.0%
BOV-P
2
162
9
5.6%
BOV-POSTM
14
63
10
15.9%
Pembrokeshire
BOV-IR-C
9
90
14
15.6%
BOV-IR-EX
4
42
6
14.3%
BOV-P
3
182
14
7.7%
Powys
BOV-IR-C
4
19
3
15.8%
BOV-IR-EX
1
10
3
30.0%
BOV-P
1
23
1
4.3%
South Wales
BOV-IR-C
3
35
16
45.7%
Wales
52
746
98
13.1%
All GB
52
746
98
13.1 %
Table 8a Number of Cattle Antibody Tests November 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
3
594
21
3.5%
Devon
1
95
6
6.3%
BOV-P
2
47
0
0
Staffordshire
BOV-P
1
30
1
3.3%
Wiltshire
BOV-P
1
189
8
4.2%
England
8
955
36
3.8%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
43
489
115
23.5%
BOV-IR-EX
6
45
13
28.9%
BOV-OTHER
3
80
4
5.0%
BOV-P
2
87
3
3.4%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
39
391
65
16.6%
BOV-IR-EX
5
32
8
25.0%
BOV-OTHER
6
240
16
6.7%
BOV-P
2
79
2
2.5%
North East Wales
2
24
6
25.0%
BOV-IR-C
29
273
44
16.1%
BOV-IR-EX
2
5
1
20.0%
BOV-OTHER
5
150
5
3.3%
BOV-P
16
1,550
63
4.1%
BOV-POSTM
57
166
35
21.1%
OTHER
1
48
4
8.3%
North West Wales
BOV-P
1
42
0
0
BOV-POSTM
4
14
4
28.6%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
137
1,482
260
17.5%
BOV-IR-EX
20
165
33
20.0%
BOV-OTHER
18
855
48
5.6%
BOV-P
17
961
45
4.7%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
27
188
36
19.1%
BOV-IR-EX
4
55
12
21.8%
BOV-OTHER
5
227
30
13.2%
BOV-P
4
77
4
5.2%
BOV-POSTM
4
8
1
12.5%
South Wales
4
14
5
35.7%
BOV-IR-C
12
112
23
20.5%
BOV-IR-EX
1
21
1
4.8%
BOV-P
2
87
1
1.1%
Wales
508
8,307
931
11.2%
All GB
516
9,262
967
10.4 %