Monthly report: Survellience Project SB4008 IGNg tests for Bovine tuberculosis - November 2018
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 147
Report Period 1st - 30th November 2018
Issued 14 December 2018

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
November
2018
%
Total 2018
%
Submissions
282
2441
Samples
Total (%)
24570
100.00 %
231932
100.00 %
England
18356
74.71 %
178364
76.90 %
Wales
6214
25.29 %
51327
22.13 %
Scotland
2241
0.97 %
Parallel Tests
PAR-CUL-N
4069
16.56 %
28707
12.38 %
PAR-CUL-P
131
0.53 %
6286
2.71 %
PAR-HERD-S
805
3.28 %
6501
2.80 %
PAR-NEW-IN
10843
44.13 %
120352
51.89 %
PAR-OTHER
1421
5.78 %
4006
1.73 %
PAR-PERSIS
6047
24.61 %
55411
23.89 %
PAR-RAPID
192
0.78 %
1507
0.65 %
Total(% of all samples)
23508
95.68 %
222770
96.05 %
Serial/Extended Tests
SER-FLEXI
1062
4.32 %
8863
3.82 %
SER-FRAUD
0
0.00 %
270
0.12 %
SER-NSR
0
0.00 %
29
0.01 %
SER-OTHER
0
0.00 %
Total(% of all samples)
1062
4.32 %
9162
3.95 %
Total
24570
100.00 %
231932
100.00 %
Retests
Total (% of all samples)
2538
10.33 %
19220
8.29 %
England
1992
8.11 %
14748
6.36 %
Wales
546
2.22 %
4271
1.84 %
Scotland
201
0.09 %
Resamples
Total (% of all samples)
1626
6.62 %
13199
5.69 %
England
1264
5.14 %
9987
4.31 %
Wales
362
1.47 %
3073
1.32 %
Scotland
139
0.06 %
Rejects
Total (% of all samples)
320
1.30 %
2243
0.97 %
England
249
1.01 %
1036
0.45 %
Wales
71
0.29 %
1207
0.52 %
Scotland
0
0.00 %
Table 1. Monthly Totals for November 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
4069
16.6 %
PAR-CUL-P
131
0.5 %
PAR-HERD-S
805
3.3 %
PAR-NEW-IN
10843
44.1 %
PAR-OTHER
1421
5.8 %
PAR-PERSIS
6047
24.6 %
PAR-RAPID
192
0.8 %
SER-FLEXI
1062
4.3 %
Total
24570
100.00 %
Figure 1. Number of samples submitted in each category during November 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
W Glamorgan
S Wales
Shropshire
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset
Shropshire
Oxon
Notts
Lincs
Leics & Rut
Isle of wight
Heref
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during November 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
Wigtown
West lothian
Perthshire
Ayrshire
Argyll & Bute
Aberdeenshire
W Glamorgan
S Wales
Shropshire
Powys
Pembs
Pembrokshire
NE Wales
Mid glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
W Midlands
Wiltshire
Warks
S Yorks
Staffs
Somerset
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Merseyside
Lincs
Leics & Rut
Kent
Isle of wight
Humberside
Herts
Heref
Hampshire
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Table 2a. Summary by County for November 2018
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
11
1,187
4.83 %
60
5.05 %
1071
90.23 %
98
8.26 %
55
4.63 %
1
0.08 %
Devon
PAR-CUL-N
10
987
4.02 %
79
8.00 %
854
86.52 %
103
10.44 %
36
3.65 %
18
1.82 %
PAR-OTHER
1
154
0.63 %
3
1.95 %
148
96.10 %
14
9.09 %
3
1.95 %
0
0.00 %
PAR-PERSIS
1
53
0.22 %
5
9.43 %
47
88.68 %
0
0.00 %
0
0.00 %
1
1.89 %
Dorset
PAR-CUL-N
4
1,580
6.43 %
124
7.85 %
1401
88.67 %
94
5.95 %
55
3.48 %
0
0.00 %
PAR-PERSIS
5
2,436
9.91 %
151
6.20 %
2167
88.96 %
184
7.55 %
114
4.68 %
4
0.16 %
Gloucs
PAR-CUL-N
8
267
1.09 %
14
5.24 %
220
82.40 %
46
17.23 %
33
12.36 %
0
0.00 %
PAR-PERSIS
1
15
0.06 %
1
6.67 %
9
60.00 %
5
33.33 %
5
33.33 %
0
0.00 %
Heref
PAR-CUL-N
1
48
0.20 %
4
8.33 %
41
85.42 %
4
8.33 %
3
6.25 %
0
0.00 %
Shropshire
PAR-NEW-IN
2
55
0.22 %
1
1.82 %
52
94.55 %
2
3.64 %
2
3.64 %
0
0.00 %
PAR-OTHER
1
12
0.05 %
0
0.00 %
7
58.33 %
5
41.67 %
5
41.67 %
0
0.00 %
PAR-PERSIS
1
133
0.54 %
3
2.26 %
121
90.98 %
28
21.05 %
9
6.77 %
0
0.00 %
Somerset
PAR-CUL-P
3
131
0.53 %
13
9.92 %
113
86.26 %
31
23.66 %
5
3.82 %
0
0.00 %
PAR-OTHER
1
15
0.06 %
1
6.67 %
14
93.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Staffs
SER-FLEXI
2
569
2.32 %
62
10.90 %
418
73.46 %
131
23.02 %
88
15.47 %
1
0.18 %
Wiltshire
PAR-NEW-IN
1
9
0.04 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
3
657
2.67 %
41
6.24 %
579
88.13 %
53
8.07 %
20
3.04 %
17
2.59 %
PAR-PERSIS
1
30
0.12 %
1
3.33 %
23
76.67 %
6
20.00 %
6
20.00 %
0
0.00 %
SER-FLEXI
4
105
0.43 %
14
13.33 %
91
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Cheshire
PAR-NEW-IN
27
2,814
11.45 %
88
3.13 %
2454
87.21 %
352
12.51 %
269
9.56 %
3
0.11 %
Derbyshire
PAR-NEW-IN
19
1,441
5.86 %
88
6.11 %
1231
85.43 %
204
14.16 %
122
8.47 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
127
0.52 %
6
4.72 %
95
74.80 %
27
21.26 %
25
19.69 %
1
0.79 %
PAR-PERSIS
2
158
0.64 %
10
6.33 %
142
89.87 %
16
10.13 %
6
3.80 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
1
26
0.11 %
4
15.38 %
21
80.77 %
3
11.54 %
1
3.85 %
0
0.00 %
PAR-OTHER
2
27
0.11 %
1
3.70 %
26
96.30 %
1
3.70 %
0
0.00 %
0
0.00 %
Notts
PAR-NEW-IN
2
5
0.02 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
Oxon
PAR-NEW-IN
14
1,153
4.69 %
68
5.90 %
987
85.60 %
127
11.01 %
98
8.50 %
0
0.00 %
PAR-PERSIS
6
411
1.67 %
33
8.03 %
353
85.89 %
39
9.49 %
25
6.08 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Warks
PAR-NEW-IN
11
1,075
4.38 %
56
5.21 %
948
88.19 %
93
8.65 %
68
6.33 %
3
0.28 %
England - LRA
Cumbria
PAR-NEW-IN
6
1,877
7.64 %
94
5.01 %
1638
87.27 %
241
12.84 %
145
7.73 %
0
0.00 %
PAR-OTHER
3
276
1.12 %
30
10.87 %
205
74.28 %
50
18.12 %
41
14.86 %
0
0.00 %
Isle of wight
PAR-NEW-IN
2
409
1.66 %
13
3.18 %
182
44.50 %
22
5.38 %
14
3.42 %
200
48.90 %
Lincs
PAR-NEW-IN
1
114
0.46 %
10
8.77 %
96
84.21 %
10
8.77 %
8
7.02 %
0
0.00 %
England
159
18356
74.71 %
1078
5.87 %
15765
85.88 %
1992
10.85 %
1264
6.89 %
249
1.36 %
Wales - High TB
Carms
PAR-HERD-S
9
71
0.29 %
15
21.13 %
45
63.38 %
23
32.39 %
10
14.08 %
1
1.41 %
PAR-OTHER
1
89
0.36 %
8
8.99 %
76
85.39 %
5
5.62 %
5
5.62 %
0
0.00 %
PAR-PERSIS
10
1,244
5.06 %
91
7.32 %
1045
84.00 %
151
12.14 %
99
7.96 %
9
0.72 %
PAR-RAPID
7
54
0.22 %
23
42.59 %
31
57.41 %
1
1.85 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
42
0.17 %
4
9.52 %
37
88.10 %
2
4.76 %
1
2.38 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
201
0.82 %
6
2.99 %
181
90.05 %
16
7.96 %
14
6.97 %
0
0.00 %
PAR-RAPID
4
21
0.09 %
2
9.52 %
18
85.71 %
3
14.29 %
1
4.76 %
0
0.00 %
SER-FLEXI
2
16
0.07 %
1
6.25 %
14
87.50 %
1
6.25 %
1
6.25 %
0
0.00 %
Gwent
PAR-PERSIS
1
7
0.03 %
2
28.57 %
3
42.86 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
5
25
0.10 %
8
32.00 %
16
64.00 %
1
4.00 %
1
4.00 %
0
0.00 %
Pembs
PAR-HERD-S
5
611
2.49 %
30
4.91 %
553
90.51 %
34
5.56 %
27
4.42 %
1
0.16 %
PAR-OTHER
1
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
10
775
3.15 %
62
8.00 %
671
86.58 %
66
8.52 %
41
5.29 %
1
0.13 %
PAR-RAPID
12
49
0.20 %
17
34.69 %
30
61.22 %
4
8.16 %
2
4.08 %
0
0.00 %
SER-FLEXI
5
165
0.67 %
32
19.39 %
126
76.36 %
12
7.27 %
6
3.64 %
1
0.61 %
Powys
PAR-HERD-S
2
13
0.05 %
2
15.38 %
9
69.23 %
2
15.38 %
2
15.38 %
0
0.00 %
PAR-PERSIS
5
295
1.20 %
28
9.49 %
239
81.02 %
33
11.19 %
28
9.49 %
0
0.00 %
PAR-RAPID
4
13
0.05 %
4
30.77 %
9
69.23 %
1
7.69 %
0
0.00 %
0
0.00 %
S Wales
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
153
0.62 %
16
10.46 %
126
82.35 %
12
7.84 %
10
6.54 %
1
0.65 %
PAR-PERSIS
1
281
1.14 %
47
16.73 %
212
75.44 %
25
8.90 %
22
7.83 %
0
0.00 %
PAR-RAPID
2
13
0.05 %
2
15.38 %
8
61.54 %
3
23.08 %
3
23.08 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-HERD-S
1
110
0.45 %
20
18.18 %
75
68.18 %
17
15.45 %
14
12.73 %
1
0.91 %
PAR-RAPID
2
5
0.02 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
76
0.31 %
8
10.53 %
59
77.63 %
10
13.16 %
9
11.84 %
0
0.00 %
NE Wales
PAR-NEW-IN
9
585
2.38 %
18
3.08 %
544
92.99 %
40
6.84 %
21
3.59 %
2
0.34 %
PAR-PERSIS
2
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
11
0.04 %
2
18.18 %
8
72.73 %
2
18.18 %
1
9.09 %
0
0.00 %
SER-FLEXI
5
89
0.36 %
6
6.74 %
78
87.64 %
5
5.62 %
5
5.62 %
0
0.00 %
Powys
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
61
0.25 %
2
3.28 %
55
90.16 %
9
14.75 %
4
6.56 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
35
0.14 %
0
0.00 %
33
94.29 %
2
5.71 %
2
5.71 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
4
948
3.86 %
38
4.01 %
835
88.08 %
54
5.70 %
22
2.32 %
53
5.59 %
NE Wales
PAR-NEW-IN
3
144
0.59 %
5
3.47 %
129
89.58 %
10
6.94 %
9
6.25 %
1
0.69 %
Wales
123
6214
25.29 %
502
8.08 %
5279
84.95 %
546
8.79 %
362
5.83 %
71
1.14 %
Grand Total
Sum:
282
24570
1580
21044
2538
1626
320
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
4
0.00 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-PERSIS
8
995
0.43 %
59
5.93 %
849
85.33 %
104
10.45 %
86
8.64 %
1
0.10 %
SER-FLEXI
2
401
0.17 %
55
13.72 %
309
77.06 %
43
10.72 %
37
9.23 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
47
6,694
2.89 %
286
4.27 %
6085
90.90 %
504
7.53 %
322
4.81 %
1
0.01 %
PAR-CUL-P
1
10
0.00 %
0
0.00 %
8
80.00 %
4
40.00 %
2
20.00 %
0
0.00 %
PAR-NEW-IN
1
121
0.05 %
18
14.88 %
98
80.99 %
14
11.57 %
5
4.13 %
0
0.00 %
PAR-OTHER
3
228
0.10 %
6
2.63 %
209
91.67 %
21
9.21 %
13
5.70 %
0
0.00 %
PAR-PERSIS
22
4,178
1.80 %
239
5.72 %
3783
90.55 %
240
5.74 %
156
3.73 %
0
0.00 %
SER-FLEXI
9
1,179
0.51 %
96
8.14 %
1028
87.19 %
54
4.58 %
47
3.99 %
8
0.68 %
Devon
PAR-CUL-N
52
7,511
3.24 %
543
7.23 %
6481
86.29 %
627
8.35 %
360
4.79 %
127
1.69 %
PAR-CUL-P
18
5,214
2.25 %
301
5.77 %
4621
88.63 %
287
5.50 %
192
3.68 %
100
1.92 %
PAR-NEW-IN
9
904
0.39 %
67
7.41 %
785
86.84 %
62
6.86 %
40
4.42 %
12
1.33 %
PAR-OTHER
6
190
0.08 %
4
2.11 %
178
93.68 %
19
10.00 %
8
4.21 %
0
0.00 %
PAR-PERSIS
35
6,143
2.65 %
319
5.19 %
5395
87.82 %
537
8.74 %
414
6.74 %
15
0.24 %
SER-FLEXI
14
2,191
0.94 %
160
7.30 %
1967
89.78 %
162
7.39 %
61
2.78 %
3
0.14 %
Dorset
PAR-CUL-N
50
8,279
3.57 %
504
6.09 %
7336
88.61 %
592
7.15 %
436
5.27 %
3
0.04 %
PAR-CUL-P
1
271
0.12 %
13
4.80 %
239
88.19 %
26
9.59 %
19
7.01 %
0
0.00 %
PAR-NEW-IN
2
636
0.27 %
8
1.26 %
558
87.74 %
73
11.48 %
70
11.01 %
0
0.00 %
PAR-OTHER
2
98
0.04 %
7
7.14 %
80
81.63 %
15
15.31 %
11
11.22 %
0
0.00 %
PAR-PERSIS
18
8,205
3.54 %
414
5.05 %
7405
90.25 %
576
7.02 %
381
4.64 %
5
0.06 %
SER-FLEXI
1
69
0.03 %
4
5.80 %
62
89.86 %
3
4.35 %
3
4.35 %
0
0.00 %
Gloucs
PAR-CUL-N
47
4,364
1.88 %
163
3.74 %
3879
88.89 %
523
11.98 %
319
7.31 %
3
0.07 %
PAR-CUL-P
9
560
0.24 %
21
3.75 %
471
84.11 %
92
xx-xxxx-xxxx %
68
12.14 %
0
0.00 %
PAR-NEW-IN
2
186
0.08 %
5
2.69 %
160
86.02 %
27
14.52 %
21
11.29 %
0
0.00 %
PAR-OTHER
2
180
0.08 %
30
16.67 %
141
78.33 %
17
9.44 %
9
5.00 %
0
0.00 %
PAR-PERSIS
5
632
0.27 %
58
9.18 %
549
86.87 %
61
9.65 %
25
3.96 %
0
0.00 %
SER-FLEXI
5
1,342
0.58 %
45
3.35 %
1247
92.92 %
76
5.66 %
49
3.65 %
1
0.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Heref
PAR-CUL-N
23
797
0.34 %
64
8.03 %
671
84.19 %
82
10.29 %
62
7.78 %
0
0.00 %
PAR-CUL-P
2
78
0.03 %
3
3.85 %
72
92.31 %
4
5.13 %
3
3.85 %
0
0.00 %
PAR-NEW-IN
3
466
0.20 %
25
5.36 %
429
92.06 %
39
8.37 %
12
2.58 %
0
0.00 %
PAR-PERSIS
4
232
0.10 %
14
6.03 %
207
89.22 %
11
4.74 %
10
4.31 %
1
0.43 %
SER-FLEXI
2
246
0.11 %
35
14.23 %
200
81.30 %
27
10.98 %
11
4.47 %
0
0.00 %
SER-FRAUD
2
38
0.02 %
1
2.63 %
36
94.74 %
2
5.26 %
1
2.63 %
0
0.00 %
Shropshire
PAR-NEW-IN
2
55
0.02 %
1
1.82 %
52
94.55 %
2
3.64 %
2
3.64 %
0
0.00 %
PAR-OTHER
8
1,142
0.49 %
64
5.60 %
1034
90.54 %
57
4.99 %
43
3.77 %
1
0.09 %
PAR-PERSIS
13
1,746
0.75 %
128
7.33 %
1556
89.12 %
111
6.36 %
62
3.55 %
0
0.00 %
Somerset
PAR-CUL-N
19
1,062
0.46 %
50
4.71 %
910
85.69 %
57
5.37 %
50
4.71 %
52
4.90 %
PAR-CUL-P
4
153
0.07 %
14
9.15 %
130
84.97 %
37
24.18 %
9
5.88 %
0
0.00 %
PAR-NEW-IN
1
22
0.01 %
1
4.55 %
16
72.73 %
5
22.73 %
5
22.73 %
0
0.00 %
PAR-OTHER
6
608
0.26 %
41
6.74 %
437
71.88 %
24
3.95 %
22
3.62 %
108
17.76 %
PAR-PERSIS
21
4,736
2.04 %
413
8.72 %
4098
86.53 %
299
6.31 %
204
4.31 %
21
0.44 %
Staffs
PAR-PERSIS
3
640
0.28 %
17
2.66 %
588
91.88 %
33
5.16 %
18
2.81 %
17
2.66 %
SER-FLEXI
4
969
0.42 %
103
10.63 %
755
77.92 %
153
15.79 %
110
11.35 %
1
0.10 %
Wiltshire
PAR-NEW-IN
2
352
0.15 %
33
9.38 %
308
87.50 %
26
7.39 %
11
3.13 %
0
0.00 %
PAR-OTHER
5
703
0.30 %
54
7.68 %
610
86.77 %
55
7.82 %
22
3.13 %
17
2.42 %
PAR-PERSIS
12
1,731
0.75 %
71
4.10 %
1605
92.72 %
78
4.51 %
55
3.18 %
0
0.00 %
SER-FLEXI
6
153
0.07 %
19
12.42 %
131
85.62 %
4
2.61 %
3
1.96 %
0
0.00 %
SER-FRAUD
2
9
0.00 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Midlands
PAR-PERSIS
1
281
0.12 %
1
0.36 %
265
94.31 %
16
5.69 %
14
4.98 %
1
0.36 %
SER-FRAUD
3
120
0.05 %
2
1.67 %
111
92.50 %
10
8.33 %
7
5.83 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
35
3,087
1.33 %
148
4.79 %
2740
88.76 %
347
11.24 %
199
6.45 %
0
0.00 %
PAR-PERSIS
6
105
0.05 %
6
5.71 %
95
90.48 %
7
6.67 %
4
3.81 %
0
0.00 %
Bucks
PAR-NEW-IN
19
2,624
1.13 %
121
4.61 %
2271
86.55 %
143
5.45 %
99
3.77 %
133
5.07 %
Cheshire
PAR-NEW-IN
197
35,774
15.42 %
1236
3.46 %
32563
91.02 %
2819
7.88 %
1971
5.51 %
4
0.01 %
PAR-PERSIS
10
2,218
0.96 %
139
6.27 %
1770
79.80 %
235
10.60 %
207
9.33 %
102
4.60 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Derbyshire
PAR-NEW-IN
113
13,734
5.92 %
507
3.69 %
12341
89.86 %
1326
9.65 %
883
6.43 %
3
0.02 %
E Sussex
PAR-NEW-IN
26
3,031
1.31 %
133
4.39 %
2710
89.41 %
297
9.80 %
185
6.10 %
3
0.10 %
PAR-PERSIS
2
158
0.07 %
10
6.33 %
142
89.87 %
16
10.13 %
6
3.80 %
0
0.00 %
Hampshire
PAR-NEW-IN
30
2,759
1.19 %
103
3.73 %
2508
90.90 %
213
7.72 %
148
5.36 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-PERSIS
1
19
0.01 %
1
5.26 %
16
84.21 %
7
36.84 %
2
10.53 %
0
0.00 %
SER-NSR
3
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
41
3,683
1.59 %
171
4.64 %
3263
88.60 %
345
9.37 %
247
6.71 %
2
0.05 %
PAR-OTHER
2
27
0.01 %
1
3.70 %
26
96.30 %
1
3.70 %
0
0.00 %
0
0.00 %
SER-NSR
1
13
0.01 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
27
1,534
0.66 %
79
5.15 %
1372
89.44 %
124
8.08 %
83
5.41 %
0
0.00 %
Notts
PAR-NEW-IN
21
1,503
0.65 %
51
3.39 %
1339
89.09 %
152
10.11 %
113
7.52 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
99
10,103
4.36 %
609
6.03 %
8819
87.29 %
973
9.63 %
666
6.59 %
9
0.09 %
PAR-PERSIS
14
2,054
0.89 %
96
4.67 %
1861
90.60 %
121
5.89 %
97
4.72 %
0
0.00 %
Warks
PAR-NEW-IN
79
6,459
2.78 %
316
4.89 %
5665
87.71 %
589
9.12 %
399
6.18 %
79
1.22 %
PAR-PERSIS
2
23
0.01 %
1
4.35 %
19
82.61 %
3
13.04 %
3
13.04 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
561
0.24 %
12
2.14 %
453
80.75 %
124
22.10 %
96
17.11 %
0
0.00 %
Cumbria
PAR-NEW-IN
37
5,315
2.29 %
179
3.37 %
4818
90.65 %
459
8.64 %
318
5.98 %
0
0.00 %
PAR-OTHER
3
276
0.12 %
30
10.87 %
205
74.28 %
50
18.12 %
41
14.86 %
0
0.00 %
PAR-PERSIS
1
330
0.14 %
11
3.33 %
304
92.12 %
18
5.45 %
15
4.55 %
0
0.00 %
Essex
PAR-NEW-IN
3
493
0.21 %
31
6.29 %
456
92.49 %
26
5.27 %
3
0.61 %
3
0.61 %
Herts
PAR-NEW-IN
4
366
0.16 %
5
1.37 %
325
88.80 %
61
16.67 %
36
9.84 %
0
0.00 %
Humberside
PAR-NEW-IN
2
99
0.04 %
8
8.08 %
86
86.87 %
8
8.08 %
5
5.05 %
0
0.00 %
Isle of wight
PAR-NEW-IN
4
555
0.24 %
21
3.78 %
297
53.51 %
57
10.27 %
37
6.67 %
200
36.04 %
Kent
PAR-NEW-IN
3
649
0.28 %
33
5.08 %
599
92.30 %
37
5.70 %
17
2.62 %
0
0.00 %
Lincs
PAR-NEW-IN
9
366
0.16 %
22
6.01 %
312
85.25 %
42
11.48 %
32
8.74 %
0
0.00 %
Merseyside
PAR-NEW-IN
2
34
0.01 %
0
0.00 %
20
58.82 %
15
44.12 %
14
41.18 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Norfolk
PAR-NEW-IN
4
455
0.20 %
9
1.98 %
409
89.89 %
48
10.55 %
37
8.13 %
0
0.00 %
N Yorks
PAR-NEW-IN
6
942
0.41 %
30
3.18 %
866
91.93 %
79
8.39 %
46
4.88 %
0
0.00 %
S Yorks
PAR-NEW-IN
2
131
0.06 %
11
8.40 %
110
83.97 %
10
7.63 %
10
7.63 %
0
0.00 %
W Sussex
PAR-NEW-IN
11
1,442
0.62 %
81
5.62 %
1300
90.15 %
87
6.03 %
61
4.23 %
0
0.00 %
PAR-PERSIS
2
297
0.13 %
6
2.02 %
277
93.27 %
15
5.05 %
14
4.71 %
0
0.00 %
England
1347
178364
76.90 %
8796
4.93 % ######
88.89 %
14748
8.27 %
9987
5.60 %
1036
0.58 %
Wales - High TB
Carms
PAR-HERD-S
38
1,244
0.54 %
91
7.32 %
1087
87.38 %
102
8.20 %
63
5.06 %
3
0.24 %
PAR-OTHER
7
142
0.06 %
14
9.86 %
122
85.92 %
7
4.93 %
6
4.23 %
0
0.00 %
PAR-PERSIS
52
5,106
2.20 %
406
7.95 %
4380
85.78 %
430
8.42 %
305
5.97 %
15
0.29 %
PAR-RAPID
92
450
0.19 %
113
25.11 %
289
64.22 %
28
6.22 %
19
4.22 %
29
6.44 %
SER-FLEXI
12
384
0.17 %
39
10.16 %
292
76.04 %
58
15.10 %
49
12.76 %
4
1.04 %
Ceredigion
PAR-HERD-S
2
6
0.00 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
PAR-OTHER
1
9
0.00 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
17
1,501
0.65 %
97
6.46 %
1328
88.47 %
86
5.73 %
72
4.80 %
4
0.27 %
PAR-RAPID
32
115
0.05 %
23
20.00 %
91
79.13 %
6
5.22 %
1
0.87 %
0
0.00 %
SER-FLEXI
10
265
0.11 %
25
9.43 %
214
80.75 %
30
11.32 %
23
8.68 %
3
1.13 %
Gwent
PAR-HERD-S
5
429
0.18 %
16
3.73 %
395
92.07 %
36
8.39 %
16
3.73 %
2
0.47 %
PAR-NEW-IN
2
17
0.01 %
1
5.88 %
13
76.47 %
4
23.53 %
3
17.65 %
0
0.00 %
PAR-OTHER
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
38
2,277
0.98 %
120
5.27 %
1982
87.04 %
306
13.44 %
172
7.55 %
3
0.13 %
PAR-RAPID
37
195
0.08 %
36
18.46 %
144
73.85 %
15
7.69 %
15
7.69 %
0
0.00 %
SER-FLEXI
6
205
0.09 %
3
1.46 %
179
87.32 %
37
18.05 %
23
11.22 %
0
0.00 %
Pembrokshire
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Pembs
PAR-HERD-S
34
3,132
1.35 %
83
2.65 %
2542
81.16 %
194
6.19 %
142
4.53 %
365
11.65 %
PAR-OTHER
5
37
0.02 %
9
24.32 %
28
75.68 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
87
7,214
3.11 %
298
4.13 %
6257
86.73 %
564
7.82 %
400
5.54 %
259
3.59 %
PAR-RAPID
133
443
0.19 %
110
24.83 %
313
70.65 %
27
6.09 %
20
4.51 %
0
0.00 %
SER-FLEXI
29
933
0.40 %
75
8.04 %
735
78.78 %
81
8.68 %
57
6.11 %
66
7.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FRAUD
2
100
0.04 %
55
55.00 %
45
45.00 %
1
1.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
16
671
0.29 %
75
11.18 %
555
82.71 %
49
7.30 %
40
5.96 %
1
0.15 %
PAR-NEW-IN
22
852
0.37 %
20
2.35 %
797
93.54 %
48
5.63 %
33
3.87 %
2
0.23 %
PAR-OTHER
5
109
0.05 %
3
2.75 %
103
94.50 %
4
3.67 %
3
2.75 %
0
0.00 %
PAR-PERSIS
48
3,921
1.69 %
157
4.00 %
3350
85.44 %
292
7.45 %
241
6.15 %
173
4.41 %
PAR-RAPID
35
124
0.05 %
18
14.52 %
94
75.81 %
13
10.48 %
11
8.87 %
1
0.81 %
SER-FLEXI
6
101
0.04 %
3
2.97 %
93
92.08 %
5
4.95 %
5
4.95 %
0
0.00 %
S Wales
PAR-PERSIS
3
8
0.00 %
1
12.50 %
5
62.50 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-RAPID
3
8
0.00 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
153
0.07 %
16
10.46 %
126
82.35 %
12
7.84 %
10
6.54 %
1
0.65 %
PAR-PERSIS
2
289
0.12 %
48
16.61 %
219
75.78 %
25
8.65 %
22
7.61 %
0
0.00 %
PAR-RAPID
4
24
0.01 %
6
25.00 %
15
62.50 %
3
12.50 %
3
12.50 %
0
0.00 %
Ceredigion
PAR-HERD-S
9
1,001
0.43 %
26
2.60 %
797
79.62 %
233
23.28 %
177
17.68 %
1
0.10 %
PAR-NEW-IN
1
68
0.03 %
4
5.88 %
63
92.65 %
3
4.41 %
1
1.47 %
0
0.00 %
PAR-PERSIS
3
153
0.07 %
22
14.38 %
116
75.82 %
24
15.69 %
15
9.80 %
0
0.00 %
PAR-RAPID
21
91
0.04 %
21
23.08 %
67
73.63 %
4
4.40 %
3
3.30 %
0
0.00 %
SER-FLEXI
5
193
0.08 %
11
5.70 %
166
86.01 %
18
9.33 %
16
8.29 %
0
0.00 %
Mid glamorgan
PAR-PERSIS
2
106
0.05 %
2
1.89 %
80
75.47 %
30
28.30 %
24
22.64 %
0
0.00 %
NE Wales
PAR-HERD-S
1
17
0.01 %
0
0.00 %
13
76.47 %
4
23.53 %
4
23.53 %
0
0.00 %
PAR-NEW-IN
124
11,919
5.14 %
208
1.75 %
10979
92.11 %
911
7.64 %
672
5.64 %
60
0.50 %
PAR-PERSIS
3
108
0.05 %
3
2.78 %
98
90.74 %
8
7.41 %
7
6.48 %
0
0.00 %
PAR-RAPID
10
32
0.01 %
5
15.63 %
25
78.13 %
2
6.25 %
1
3.13 %
1
3.13 %
SER-FLEXI
9
171
0.07 %
13
7.60 %
149
87.13 %
10
5.85 %
9
5.26 %
0
0.00 %
Powys
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
PAR-NEW-IN
8
398
0.17 %
15
3.77 %
364
91.46 %
38
9.55 %
19
4.77 %
0
0.00 %
PAR-RAPID
3
8
0.00 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
82
0.04 %
3
3.66 %
74
90.24 %
13
15.85 %
5
6.10 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Glamorgan
PAR-OTHER
3
46
0.02 %
5
10.87 %
39
84.78 %
2
4.35 %
2
4.35 %
0
0.00 %
SER-FLEXI
2
5
0.00 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
18
1,815
0.78 %
46
2.53 %
1621
89.31 %
153
8.43 %
94
5.18 %
54
2.98 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
38
4,575
1.97 %
97
2.12 %
4059
88.72 %
344
7.52 %
260
5.68 %
159
3.48 %
PAR-OTHER
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
5
0.00 %
0
0.00 %
2
40.00 %
3
60.00 %
3
60.00 %
0
0.00 %
PAR-RAPID
3
14
0.01 %
2
14.29 %
12
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
46
0.02 %
0
0.00 %
45
97.83 %
2
4.35 %
1
2.17 %
0
0.00 %
Wales
1063
51327
22.13 %
2452
4.78 %
44595
86.88 %
4271
8.32 %
3073
5.99 %
1207
2.35 %
Scotland - Scotland
Aberdeenshire
PAR-NEW-IN
2
15
0.01 %
0
0.00 %
14
93.33 %
1
6.67 %
1
6.67 %
0
0.00 %
Argyll & Bute
PAR-NEW-IN
12
887
0.38 %
68
7.67 %
766
86.36 %
77
8.68 %
53
5.98 %
0
0.00 %
SER-FLEXI
1
10
0.00 %
1
10.00 %
8
80.00 %
1
10.00 %
1
10.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
7
579
0.25 %
37
6.39 %
496
85.66 %
66
11.40 %
46
7.94 %
0
0.00 %
PAR-OTHER
1
46
0.02 %
39
84.78 %
7
15.22 %
1
2.17 %
0
0.00 %
0
0.00 %
Perthshire
PAR-NEW-IN
3
217
0.09 %
17
7.83 %
187
86.18 %
22
10.14 %
13
5.99 %
0
0.00 %
West lothian
PAR-NEW-IN
2
142
0.06 %
1
0.70 %
137
96.48 %
4
2.82 %
4
2.82 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
345
0.15 %
18
5.22 %
306
88.70 %
29
8.41 %
21
6.09 %
0
0.00 %
Scotland
31
2241
0.97 %
181
8.08 %
1921
85.72 %
201
8.97 %
139
6.20 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
11429
######
19220
13199
2243
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol November 2018
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
153
17682
96.3%
1002
5.7%
15256
86.3%
1861
10.5%
1176
6.7%
248
1.4%
Serial/Extended
6
674
3.7%
76
11.3%
509
75.5%
131
19.4%
88
13.1%
1
0.1%
Total
159
18356
100.0%
1078
5.9%
15765
85.9%
1992
10.9%
1264
6.9%
249
1.4%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
108
5826
93.8%
451
7.7%
4965
85.2%
516
8.9%
340
5.8%
70
1.2%
Serial/Extended
15
388
6.2%
51
13.1%
314
80.9%
30
7.7%
22
5.7%
1
0.3%
Total
123
6214
100.0%
502
8.1%
5279
85.0%
546
8.8%
362
5.8%
71
1.1%
GB
Parallel
261
23508
95.7%
1453
6.2%
20221
86.0%
2377
10.1%
1516
6.4%
318
1.4%
Serial/Extended
21
1062
4.3%
127
12.0%
823
77.5%
161
15.2%
110
10.4%
2
0.2%
Total
282
24570
100.0%
1580
6.4%
21044
85.6%
2538
10.3%
1626
6.6%
320
1.3%
159.00
123.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2018)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
96.2%
8276
4.8% 152658
89.0%
14214
8.3%
9658
5.6%
1023
0.6%
Serial/Extended
55
6749
3.8%
520
7.7%
5887
87.2%
534
7.9%
329
4.9%
13
0.2%
Total
xx-xxxx-xxxx
100.0%
8796
4.9% 158545
88.9%
14748
8.3%
9987
5.6%
1036
0.6%
Scotland
Parallel
30
2231
99.6%
180
8.1%
1913
85.7%
200
9.0%
138
6.2%
0
0.0 %
Serial/Extended
1
10
0.4%
1
0.0%
8
80.0%
1
10.0%
1
10.0%
0
0.0 %
Total
31
2241
100.0%
181
8.1%
1921
85.7%
201
9.0%
139
6.2%
0
0.0 %
Wales
Parallel
980
48924
95.3%
2228
4.6%
42673
87.2%
4028
8.2%
2889
5.9%
1134
2.3%
Serial/Extended
83
2403
4.7%
224
9.3%
1922
80.0%
243
10.1%
184
7.7%
73
3.0%
Total
1063
51327
100.0%
2452
4.8%
44595
86.9%
4271
8.3%
3073
6.0%
1207
2.4%
GB
Parallel
xx-xxxx-xxxx
96.0% 10684
4.8% 197244
88.5%
18442
8.3%
12685
5.7%
2157
1.0%
Serial/Extended
139
9162
4.0%
745
8.1%
7817
85.3%
778
8.5%
514
5.6%
86
0.9%
Total
xx-xxxx-xxxx
100.0% 11429
4.9% 205061
88.4%
19220
8.3%
13199
5.7%
2243
1.0%
#######
#######
31.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
21
1062
4.3%
127
12.0%
823
77.5%
161
15.2 %
110
10.4 %
2
0.2 %
Parallel - Herd or Group Slaughter
17
805
3.3%
67
8.3%
682
84.7%
76
9.4 %
53
6.6 %
3
0.4 %
Parallel New Infection
105
10843
44.1%
491
4.5%
9278
85.6%
1197
11.0 %
811
7.5 %
263
2.4 %
Parallel - new infection cull area
34
4069
16.6%
281
6.9%
3587
88.2%
345
8.5 %
182
4.5 %
19
0.5 %
Parallel - Other
16
1421
5.8%
102
7.2%
1215
85.5%
142
10.0 %
86
6.1 %
18
1.3 %
Parallel Persistent Infection
48
6047
24.6%
440
7.3%
5221
86.3%
571
9.4 %
371
6.1 %
15
0.2 %
Parallel - persistent infection cull area
3
131
0.5%
13
9.9%
113
86.3%
31
23.7 %
5
3.8 %
0
Parallel - Rapid Testing Two-times IRs
38
192
0.8%
59
30.7%
125
65.1%
15
7.8 %
8
4.2 %
0
Total
282
24570
100.0%
1580
6.4%
21044
85.6%
2538
10.3 %
1626
6.6 %
320
1.3 %
Table 4a. Summaryof IFN Gamma results by submission reason (November 2018)
England - HRA
Extended Flexible IFN-GAMMA test
6
674
2.7%
76
11.3%
509
75.5%
131
19.4 %
88
13.1 %
1
0.1 %
Parallel New Infection
3
64
0.3%
1
1.6%
60
93.8%
3
4.7 %
3
4.7 %
0
Parallel - new infection cull area
34
4069
16.6%
281
6.9%
3587
88.2%
345
8.5 %
182
4.5 %
19
0.5 %
Parallel - Other
6
838
3.4%
45
5.4%
748
89.3%
72
8.6 %
28
3.3 %
17
2.0 %
Parallel Persistent Infection
9
2667
10.9%
161
6.0%
2367
88.8%
223
8.4 %
134
5.0 %
5
0.2 %
Parallel - persistent infection cull area
3
131
0.5%
13
9.9%
113
86.3%
31
23.7 %
5
3.8 %
0
England - HRA
61
8443
34.4%
577
6.8%
7384
87.5%
805
9.5 %
440
5.2 %
42
0.5 %
England - Edge
Parallel New Infection
76
6641
27.0%
310
4.7%
5739
86.4%
808
12.2 %
585
8.8 %
7
0.1 %
Parallel - Other
2
27
0.1%
1
3.7%
26
96.3%
1
3.7 %
0
0
Parallel Persistent Infection
8
569
2.3%
43
7.6%
495
87.0%
55
9.7 %
31
5.4 %
0
England - Edge
86
7237
29.5%
354
4.9%
6260
86.5%
864
11.9 %
616
8.5 %
7
0.1 %
England - LRA
Parallel New Infection
9
2400
9.8%
117
4.9%
1916
79.8%
273
11.4 %
167
7.0 %
200
8.3 %
Parallel - Other
3
276
1.1%
30
10.9%
205
74.3%
50
18.1 %
41
14.9 %
0
England - LRA
12
2676
10.9%
147
5.5%
2121
79.3%
323
12.1 %
208
7.8 %
200
7.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
9
223
0.9%
37
16.6%
177
79.4%
15
6.7 %
8
3.6 %
1
0.4 %
Parallel - Herd or Group Slaughter
16
695
2.8%
47
6.8%
607
87.3%
59
8.5 %
39
5.6 %
2
0.3 %
Parallel - Other
2
92
0.4%
10
10.9%
77
83.7%
5
5.4 %
5
5.4 %
0
Parallel Persistent Infection
28
2523
10.3%
189
7.5%
2140
84.8%
268
10.6 %
184
7.3 %
10
0.4 %
Parallel - Rapid Testing Two-times IRs
32
162
0.7%
54
33.3%
104
64.2%
10
6.2 %
4
2.5 %
0
Wales - High TB
87
3695
15.0%
337
9.1%
3105
84.0%
357
9.7 %
240
6.5 %
13
0.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
6
165
0.7%
14
8.5%
137
83.0%
15
9.1 %
14
8.5 %
0
Parallel - Herd or Group Slaughter
1
110
0.4%
20
18.2%
75
68.2%
17
15.5 %
14
12.7 %
1
0.9 %
Parallel New Infection
10
646
2.6%
20
3.1%
599
92.7%
49
7.6 %
25
3.9 %
2
0.3 %
Parallel - Other
3
188
0.8%
16
8.5%
159
84.6%
14
7.4 %
12
6.4 %
1
0.5 %
Parallel Persistent Infection
3
288
1.2%
47
16.3%
219
76.0%
25
8.7 %
22
7.6 %
0
Parallel - Rapid Testing Two-times IRs
6
30
0.1%
5
16.7%
21
70.0%
5
16.7 %
4
13.3 %
0
Wales - Intermediate TB
29
1427
5.8%
122
8.5%
1210
84.8%
125
8.8 %
91
6.4 %
4
0.3 %
Wales - Low TB
Parallel New Infection
7
1092
4.4%
43
3.9%
964
88.3%
64
5.9 %
31
2.8 %
54
4.9 %
Wales - Low TB
7
1092
4.4%
43
3.9%
964
88.3%
64
5.9 %
31
2.8 %
54
4.9 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
125
8863
3.8%
687
7.8%
7584
85.6%
765
8.6 %
506
5.7 %
86
1.0 %
Parallel - Herd or Group Slaughter
106
6501
2.8%
291
4.5%
5393
83.0%
620
9.5 %
444
6.8 %
373
5.7 %
Parallel New Infection
1044 120352
51.9%
4609
3.8%
107924
89.7%
10342
8.6 %
7096
5.9 %
723
0.6 %
Parallel - new infection cull area
238
28707
12.4%
1610
5.6%
25362
88.3%
2385
8.3 %
1549
5.4 %
186
0.6 %
Parallel - Other
67
4006
1.7%
327
8.2%
3358
83.8%
289
7.2 %
194
4.8 %
127
3.2 %
Parallel Persistent Infection
436
55411
23.9%
3157
5.7%
48601
87.7%
4258
7.7 %
3036
5.5 %
617
1.1 %
Parallel - persistent infection cull area
35
6286
2.7%
352
5.6%
5541
88.1%
450
7.2 %
293
4.7 %
100
1.6 %
Parallel - Rapid Testing Two-times IRs
376
1507
0.6%
338
22.4%
1065
70.7%
98
6.5 %
73
4.8 %
31
2.1 %
Serial - Anomalous Reaction
10
270
0.1%
58
21.5%
204
75.6%
13
4.8 %
8
3.0 %
0
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
11429
4.9%
205061
88.4%
19220
8.3 %
13199
5.7 %
2243
1.0 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2018)
England - HRA
Extended Flexible IFN-GAMMA test
43
6550
2.8%
517
7.9%
5699
87.0%
522
8.0 %
321
4.9 %
13
0.2 %
Parallel New Infection
22
2742
1.2%
158
5.8%
2406
87.7%
248
9.0 %
166
6.1 %
12
0.4 %
Parallel - new infection cull area
238
28707
12.4%
1610
5.6%
25362
88.3%
2385
8.3 %
1549
5.4 %
186
0.6 %
Parallel - Other
33
3153
1.4%
206
6.5%
2691
85.3%
210
6.7 %
130
4.1 %
126
4.0 %
Parallel Persistent Infection
142
29519
12.7%
1733
5.9%
26300
89.1%
2066
7.0 %
1425
4.8 %
61
0.2 %
Parallel - persistent infection cull area
35
6286
2.7%
352
5.6%
5541
88.1%
450
7.2 %
293
4.7 %
100
1.6 %
Serial - Anomalous Reaction
8
170
0.1%
3
1.8%
159
93.5%
12
7.1 %
8
4.7 %
0
England - Edge
Parallel New Infection
687
84291
36.3%
3474
4.1%
75591
89.7%
7328
8.7 %
4993
5.9 %
233
0.3 %
Parallel - Other
4
29
0.0%
1
3.4%
27
93.1%
2
6.9 %
1
3.4 %
0
Parallel Persistent Infection
35
4577
2.0%
253
5.5%
3903
85.3%
389
8.5 %
319
7.0 %
102
2.2 %
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
England - LRA
Parallel New Infection
90
11408
4.9%
442
3.9%
10051
88.1%
1053
9.2 %
712
6.2 %
203
1.8 %
Parallel - Other
3
276
0.1%
30
10.9%
205
74.3%
50
18.1 %
41
14.9 %
0
Parallel Persistent Infection
3
627
0.3%
17
2.7%
581
92.7%
33
5.3 %
29
4.6 %
0
England
xx-xxxx-xxxx
76.9%
8796
4.9%
158545
88.9%
14748
8.3 %
9987
5.6 %
1036
0.6 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2018) Cont.
Wales - High TB
Extended Flexible IFN-GAMMA test
63
1888
0.8%
145
7.7%
1513
80.1%
211
11.2 %
157
8.3 %
73
3.9 %
Parallel - Herd or Group Slaughter
95
5482
2.4%
265
4.8%
4583
83.6%
383
7.0 %
263
4.8 %
371
6.8 %
Parallel New Infection
24
869
0.4%
21
2.4%
810
93.2%
52
6.0 %
36
4.1 %
2
0.2 %
Parallel - Other
21
301
0.1%
29
9.6%
262
87.0%
12
4.0 %
10
3.3 %
0
Parallel Persistent Infection
245
20027
8.6%
1079
5.4%
17302
86.4%
xx-xxxx-xxxx
8.4 %
1192
6.0 %
454
2.3 %
Parallel - Rapid Testing Two-times IRs
334
1337
0.6%
302
22.6%
939
70.2%
89
6.7 %
66
4.9 %
30
2.2 %
Serial - Anomalous Reaction
2
100
0.0%
55
55.0%
45
45.0%
1
1.0 %
0
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
16
369
0.2%
24
6.5%
319
86.4%
29
7.9 %
26
7.0 %
0
Parallel - Herd or Group Slaughter
11
1019
0.4%
26
2.6%
810
79.5%
237
23.3 %
181
17.8 %
2
0.2 %
Parallel New Infection
136
12467
5.4%
230
1.8%
11480
92.1%
965
7.7 %
697
5.6 %
60
0.5 %
Parallel - Other
4
199
0.1%
21
10.6%
165
82.9%
14
7.0 %
12
6.0 %
1
0.5 %
Parallel Persistent Infection
10
656
0.3%
75
11.4%
513
78.2%
87
13.3 %
68
10.4 %
0
Parallel - Rapid Testing Two-times IRs
38
155
0.1%
34
21.9%
113
72.9%
9
5.8 %
7
4.5 %
1
0.6 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
46
0.0%
0
0.0 %
45
97.8%
2
4.3 %
1
2.2 %
0
Parallel New Infection
56
6390
2.8%
143
2.2%
5680
88.9%
497
7.8 %
354
5.5 %
213
3.3 %
Parallel - Other
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
2
40.0%
3
60.0 %
3
60.0 %
0
Parallel - Rapid Testing Two-times IRs
4
15
0.0%
2
13.3%
13
86.7%
0
0
0
Wales
1063
51327
22.1%
2452
4.8%
44595
86.9%
4271
8.3 %
3073
6.0 %
1207
2.4 %
Scotland - Scotland
Extended Flexible IFN-GAMMA test
1
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Parallel New Infection
29
2185
0.9%
141
6.5%
1906
87.2%
199
9.1 %
138
6.3 %
0
Parallel - Other
1
46
0.0%
39
84.8%
7
15.2%
1
2.2 %
0
0
Scotland
31
2241
1.0%
181
8.1%
1921
85.7%
201
9.0 %
139
6.2 %
0

Table 5a. Summary of PM and culture results for IFNg positive animals.
Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2017 December
114
9478
81
668
43
0
0
0
0
29
2
0
1
0
562
0
28
1
1
2018 January
207
19678
140
969
5
3
0
0
0
0
80
3
0
5
8
756
0
84
0
24
February
206
20791
130
1229
3
22
0
0
0
0
72
7
1
8
0
1073
1
35
0
5
March
189
17292
131
1199
1
3
0
0
0
0
121
6
0
4
0
984
0
72
1
2
April
195
20517
118
953
4
16
0
0
0
0
116
1
0
0
0
748
0
66
1
1
May
250
21066
131
947
2
2
0
0
0
0
121
1
0
4
0
780
0
35
1
1
June
187
18891
110
701
2
2
0
0
0
0
78
2
0
12
0
579
0
25
0
0
July
207
24111
119
806
1
12
0
0
0
0
67
4
0
2
0
675
3
38
0
2
August
228
19599
120
682
30
30
0
0
0
0
59
2
0
4
1
516
0
31
0
0
September
216
21441
137
946
6
6
0
0
0
0
33
0
0
2
1
840
7
44
1
4
October
274
23976
152
1417
12
12
0
0
0
0
99
0
3
2
0
1224
40
1
0
0
November
282
24570
192
1580
21
620
0
0
0
0
28
0
9
0
0
637
83
1
0
0
Totals (last
12 months)
2555
241410
1561
12097
87
771
0
0
0
0
903
28
13
44
10
9374
134
460
5
40

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)
Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1393
185563
966
9240
491
0
0
0
0
769
16
11
26
0
7644
20
41
1
2
Scotland
33
2727
22
240
30
0
0
0
0
25
4
0
0
8
105
0
37
0
27
Wales
1129
53120
573
2617
250
0
0
0
0
109
8
2
18
2
1625
114
382
4
11
Totals
2555
241410
1561
12097
771
0
0
0
0
903
28
13
44
10
9374
134
460
5
40

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
40
106
53
87
48
87
50
86
47
85
65
95
45
72
69
75
39
52
37
53
45
83
39
68
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2016
2017
2018
IFNg Positive herds
IFNg Negative herds
7789
542
6091
549
7323
420
9568
453
9409
536
8947
398
10544
376
8010
424
7065
288
6507
334
8559
647
7448
325
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2016
2017
2018
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2016
2017
2018
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
80
90
Wiltshire
W Glamorgan
Warks
S Wales
Staffs
Somerset
Shropshire
Powys
Pembs
Oxon
Notts
NE Wales
Lincs
Leics & Rut
Isle of wight
Heref
Gwynedd
Gwent
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during November 2018 (% submission per
County)
County
%Resampled %Retested
Carms
7.70 %
11.40 %
Ceredigion
9.09 %
10.96 %
Cheshire
9.56 %
12.51 %
Corn & Scilly
4.63 %
8.26 %
Cumbria
8.64 %
13.52 %
Derbyshire
8.47 %
14.16 %
Devon
3.27 %
9.80 %
Dorset
4.21 %
6.92 %
E Sussex
10.88 %
15.09 %
Gloucs
13.48 %
18.09 %
Gwent
9.38 %
9.38 %
Gwynedd
2.32 %
5.70 %
Heref
6.25 %
8.33 %
Isle of wight
3.42 %
5.38 %
Leics & Rut
1.89 %
7.55 %
Lincs
7.02 %
8.77 %
NE Wales
4.31 %
6.82 %
Notts
40.00 %
40.00 %
Oxon
7.86 %
10.61 %
Pembs
4.74 %
7.24 %
Powys
9.32 %
11.18 %
Shropshire
7.66 %
xx-xxxx-xxxx %
Somerset
3.42 %
21.23 %
Staffs
15.47 %
23.02 %
S Wales
Warks
6.33 %
8.65 %
W Glamorgan
5.71 %
5.71 %
Wiltshire
3.37 %
7.49 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
17996
1078
6.0%
18356
1078
5.9%
Wales
6081
502
8.3%
6214
502
8.1%
GB
24076
1580
6.6%
24570
1580
6.4%
Table 6a (i): Number of animals and submissions tested in November 2018
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
154045
8796
5.7%
178364
8796
4.9%
Wales
44614
2443
5.5%
51327
2452
4.8%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
200719
11420
5.7%
231932
11429
4.9%
Table 6b (i): Number of animals and submissions tested in 2018 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
8519
666
7.8%
8696
666
7.7%
Wales
1592
183
11.5%
1615
183
11.3%
Scotland
342
112
32.7%
355
112
31.5%
GB
10453
961
9.2%
10666
961
9.0%
Table 6a (ii): Number of animals and submissions tested in November 2017
Table 6b (ii): Number of animals and submissions tested in 2017 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
72323
4650
6.4%
91330
4650
5.1%
Wales
30793
2149
7.0%
33867
2149
6.3%
Scotland
2707
169
6.2%
2781
169
6.1%
GB
105820
6968
6.6%
127978
6968
5.4%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cheshire
PRV-SP
1
1
0
Hampshire
PRV-SP
1
3
0
West Sussex
PRV-SP
1
1
0
Total
3
5
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples November 2018
Table 7b Number of private samples 2018
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
2
0
Buckinghamshire
PRV-SE
1
1
0
Cheshire
PRV-SP
3
3
1
33.33 %
Cornwall and Isles of Scilly
PRV-SE
1
3
0
PRV-SP
1
1
0
Devon
PRV-SE
1
2
1
50.00 %
PRV-SP
1
2
0
Dorset
PRV-SE
4
25
6
24.00 %
PRV-SP
1
1
0
East Sussex
PRV-SE
1
1
0
PRV-SP
1
1
0
Hampshire
PRV-SE
2
6
1
16.67 %
PRV-SP
1
3
0
Northern Somerset & South Glou
PRV-SE
2
46
8
17.39 %
Oxfordshire
PRV-SE
1
38
4
10.53 %
Somerset excl North
PRV-SE
3
46
3
6.52 %
PRV-SP
1
1
0
Suffolk
PRV-SP
2
11
0
West Sussex
PRV-SP
1
1
0
Total
29
194
24
12.37 %
Isle of Man
SER-IOM
16
49
1
2.04 %

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
12
1
8.3 %
North East Wales
2
66
4
6.1 %
Powys
1
16
1
6.3 %
TC0077
Sum:
94
6
6.4 %
Table 8a Number of Cattle Antibody Tests November 2018
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Argyll and Bute
1
3
3
100.0 %
Carmarthenshire
3
182
5
2.7 %
Ceredigion
1
85
4
4.7 %
North East Wales
2
66
4
6.1 %
Pembrokeshire
3
201
10
5.0 %
Powys
2
361
4
1.1 %
South Wales
5
199
17
8.5 %
TC0077
Sum:
1,097
47
4.3 %
Table 8b Number of Cattle Antibody Tests 2018