Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - July 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 179
Report Period 1st - 31st July 2021
Issued 10 August 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
PAR-RECUR England - parallel blood testing of
skin test-negative cattle in breakdown herds (in
HRA & 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown England - YES
Wales - NO (APHA
discretion)
PAR-POSTM
Post movement blood test of cattle (coupled
with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Test Criteria

Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
July 2021
%
Total 2021
%
Submissions
298
2523
Samples
Total (%)
24841
100.00 %
201002
100.00 %
England
20377
82.03 %
156713
77.97 %
Wales
4464
17.97 %
43231
21.51 %
Scotland
1058
0.53 %
Parallel Tests
PAR-CUL-N
13410
53.98 %
94239
46.88 %
PAR-CUL-P
1741
7.01 %
7743
3.85 %
PAR-HERD-S
229
0.92 %
1446
0.72 %
PAR-IR-EX
17
0.07 %
248
0.12 %
PAR-IR-SEV
320
1.29 %
1411
0.70 %
PAR-NEW-IN
4613
18.57 %
57389
28.55 %
PAR-OTHER
106
0.43 %
5532
2.75 %
PAR-PERSIS
3854
15.51 %
23030
11.46 %
PAR-POSTM
35
0.14 %
45
0.02 %
PAR-RAPID
59
0.24 %
637
0.32 %
PAR-RECUR
32
0.13 %
5169
2.57 %
Total(% of all samples)
24416
98.29 %
196889
97.95 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
19
0.01 %
SER-FLEXI
425
1.71 %
4038
2.01 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
52
0.03 %
Total(% of all samples)
425
1.71 %
4113
2.05 %
Total
24841
100.00 %
201002
100.00 %
Retests
Total (% of all samples)
2618
10.54 %
14592
7.26 %
England
2326
9.36 %
11805
5.87 %
Wales
292
1.18 %
2708
1.35 %
Scotland
79
0.04 %
Resamples
Total (% of all samples)
2025
8.15 %
12123
6.03 %
England
1808
7.28 %
9882
4.92 %
Wales
217
0.87 %
2172
1.08 %
Scotland
69
0.03 %
Rejects
Total (% of all samples)
61
0.25 %
609
0.30 %
England
58
0.23 %
493
0.25 %
Wales
3
0.01 %
106
0.05 %
Scotland
0
0.00 %
10
0.00 %
Table 1. Monthly Totals for July 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
13410
54.0 %
PAR-CUL-P
1741
7.0 %
PAR-HERD-S
229
0.9 %
PAR-IR-EX
17
0.1 %
PAR-IR-SEV
320
1.3 %
PAR-NEW-IN
4613
18.6 %
PAR-OTHER
106
0.4 %
PAR-PERSIS
3854
15.5 %
PAR-POSTM
35
0.1 %
PAR-RAPID
59
0.2 %
PAR-RECUR
32
0.1 %
SER-FLEXI
425
1.7 %
Total
24841
100.00 %
Figure 1. Number of samples submitted in each category during July 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Heref
Gloucs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Beds
Avon
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during July 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
1
33
0.13 %
0
0.00 %
12
36.36 %
23
69.70 %
21
63.64 %
0
0.00 %
PAR-HERD-S
1
219
0.88 %
28
12.79 %
186
84.93 %
8
3.65 %
5
2.28 %
0
0.00 %
PAR-OTHER
2
56
0.23 %
3
5.36 %
38
67.86 %
20
35.71 %
15
26.79 %
0
0.00 %
PAR-PERSIS
1
384
1.55 %
21
5.47 %
330
85.94 %
46
11.98 %
33
8.59 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
5
423
1.70 %
13
3.07 %
366
86.52 %
65
15.37 %
44
10.40 %
0
0.00 %
PAR-CUL-P
2
969
3.90 %
93
9.60 %
829
85.55 %
63
6.50 %
47
4.85 %
0
0.00 %
Devon
PAR-CUL-N
45
4,762
19.17 %
241
5.06 %
4060
85.26 %
545
11.44 %
418
8.78 %
43
0.90 %
PAR-CUL-P
1
305
1.23 %
18
5.90 %
254
83.28 %
48
15.74 %
33
10.82 %
0
0.00 %
PAR-PERSIS
1
9
0.04 %
0
0.00 %
6
66.67 %
3
33.33 %
3
33.33 %
0
0.00 %
SER-FLEXI
1
11
0.04 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
Dorset
PAR-CUL-N
7
1,748
7.04 %
48
2.75 %
1569
89.76 %
176
10.07 %
131
7.49 %
0
0.00 %
Gloucs
PAR-CUL-N
9
1,337
5.38 %
43
3.22 %
1135
84.89 %
200
14.96 %
156
11.67 %
3
0.22 %
PAR-PERSIS
5
922
3.71 %
81
8.79 %
776
84.16 %
92
9.98 %
64
6.94 %
1
0.11 %
Heref
PAR-CUL-N
2
155
0.62 %
2
1.29 %
143
92.26 %
13
8.39 %
10
6.45 %
0
0.00 %
Shropshire
PAR-CUL-N
3
409
1.65 %
3
0.73 %
393
96.09 %
12
2.93 %
6
1.47 %
7
1.71 %
Somerset excl N
PAR-CUL-N
14
990
3.99 %
53
5.35 %
872
88.08 %
90
9.09 %
64
6.46 %
1
0.10 %
PAR-CUL-P
4
33
0.13 %
1
3.03 %
25
75.76 %
9
27.27 %
7
21.21 %
0
0.00 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Staffs
PAR-CUL-N
8
1,644
6.62 %
19
1.16 %
1514
92.09 %
145
8.82 %
111
6.75 %
0
0.00 %
Wiltshire
PAR-CUL-N
2
406
1.63 %
7
1.72 %
348
85.71 %
64
15.76 %
51
12.56 %
0
0.00 %
PAR-CUL-P
3
401
1.61 %
5
1.25 %
379
94.51 %
19
4.74 %
17
4.24 %
0
0.00 %
PAR-PERSIS
1
26
0.10 %
0
0.00 %
19
73.08 %
7
26.92 %
7
26.92 %
0
0.00 %
England - Edge
Cheshire
PAR-CUL-N
7
1,536
6.18 %
47
3.06 %
1422
92.58 %
88
5.73 %
67
4.36 %
0
0.00 %
PAR-NEW-IN
3
15
0.06 %
0
0.00 %
8
53.33 %
7
46.67 %
7
46.67 %
0
0.00 %
PAR-PERSIS
1
61
0.25 %
6
9.84 %
48
78.69 %
7
11.48 %
7
11.48 %
0
0.00 %
Derbyshire
PAR-NEW-IN
13
1,010
4.07 %
51
5.05 %
824
81.58 %
177
17.52 %
135
13.37 %
0
0.00 %
Table 2a. Summary by County for July 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for July 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
1
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Northants
PAR-NEW-IN
6
105
0.42 %
1
0.95 %
83
79.05 %
26
24.76 %
21
20.00 %
0
0.00 %
Notts
PAR-NEW-IN
2
194
0.78 %
3
1.55 %
170
87.63 %
27
13.92 %
21
10.82 %
0
0.00 %
Oxon
PAR-NEW-IN
5
265
1.07 %
8
3.02 %
218
82.26 %
43
16.23 %
39
14.72 %
0
0.00 %
PAR-PERSIS
3
855
3.44 %
29
3.39 %
789
92.28 %
41
4.80 %
37
4.33 %
0
0.00 %
Warks
PAR-NEW-IN
5
362
1.46 %
11
3.04 %
319
88.12 %
39
10.77 %
32
8.84 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
1
228
0.92 %
2
0.88 %
180
78.95 %
54
23.68 %
43
18.86 %
3
1.32 %
Cumbria
PAR-NEW-IN
1
295
1.19 %
1
0.34 %
178
60.34 %
124
42.03 %
116
39.32 %
0
0.00 %
Lincs
PAR-NEW-IN
3
194
0.78 %
1
0.52 %
161
82.99 %
36
18.56 %
32
xx-xxxx-xxxx %
0
0.00 %
N Yorks
PAR-NEW-IN
1
9
0.04 %
0
0.00 %
3
33.33 %
7
77.78 %
6
66.67 %
0
0.00 %
England
171
20377
82.03 %
841
4.13 %
17670
86.72 %
2326
11.41 %
1808
8.87 %
58
0.28 %
Wales - High TB
Carms
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
8
85
0.34 %
22
25.88 %
60
70.59 %
4
4.71 %
3
3.53 %
0
0.00 %
PAR-PERSIS
2
428
1.72 %
22
5.14 %
394
92.06 %
14
3.27 %
12
2.80 %
0
0.00 %
PAR-RAPID
8
24
0.10 %
7
29.17 %
16
66.67 %
1
4.17 %
1
4.17 %
0
0.00 %
SER-FLEXI
1
68
0.27 %
3
4.41 %
63
92.65 %
2
2.94 %
2
2.94 %
0
0.00 %
Ceredigion
PAR-IR-SEV
4
55
0.22 %
12
21.82 %
41
74.55 %
3
5.45 %
2
3.64 %
0
0.00 %
PAR-RAPID
2
5
0.02 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-SEV
13
139
0.56 %
31
22.30 %
103
74.10 %
7
5.04 %
5
3.60 %
0
0.00 %
PAR-PERSIS
8
260
1.05 %
14
5.38 %
238
91.54 %
10
3.85 %
8
3.08 %
0
0.00 %
PAR-RAPID
4
16
0.06 %
4
25.00 %
11
68.75 %
2
12.50 %
1
6.25 %
0
0.00 %
SER-FLEXI
4
159
0.64 %
5
3.14 %
148
93.08 %
13
8.18 %
6
3.77 %
0
0.00 %
Powys
PAR-HERD-S
1
10
0.04 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-IR-EX
2
15
0.06 %
4
26.67 %
11
73.33 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
26
0.10 %
2
7.69 %
23
88.46 %
1
3.85 %
1
3.85 %
0
0.00 %
PAR-PERSIS
3
298
1.20 %
27
9.06 %
222
74.50 %
52
17.45 %
49
xx-xxxx-xxxx %
0
0.00 %
PAR-RAPID
6
12
0.05 %
4
33.33 %
7
58.33 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for July 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
S Wales
PAR-IR-SEV
1
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
NE Wales
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
11
0.04 %
6
54.55 %
5
45.45 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
18
1,748
7.04 %
52
2.97 %
1624
92.91 %
102
5.84 %
69
3.95 %
3
0.17 %
PAR-PERSIS
6
610
2.46 %
7
1.15 %
588
96.39 %
26
4.26 %
15
2.46 %
0
0.00 %
PAR-POSTM
3
11
0.04 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
SER-FLEXI
2
145
0.58 %
1
0.69 %
141
97.24 %
6
4.14 %
3
2.07 %
0
0.00 %
Powys
PAR-NEW-IN
2
120
0.48 %
0
0.00 %
115
95.83 %
5
4.17 %
5
4.17 %
0
0.00 %
PAR-POSTM
3
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-RECUR
1
24
0.10 %
0
0.00 %
18
75.00 %
6
25.00 %
6
25.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
3
63
0.25 %
3
4.76 %
43
68.25 %
22
34.92 %
17
26.98 %
0
0.00 %
PAR-OTHER
1
50
0.20 %
0
0.00 %
49
98.00 %
2
4.00 %
1
2.00 %
0
0.00 %
PAR-POSTM
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
42
0.17 %
1
2.38 %
37
88.10 %
5
11.90 %
4
9.52 %
0
0.00 %
NE Wales
PAR-POSTM
6
16
0.06 %
0
0.00 %
16
100.00 %
1
6.25 %
0
0.00 %
0
0.00 %
Wales
127
4464
17.97 %
236
5.29 %
4008
89.78 %
292
6.54 %
217
4.86 %
3
0.07 %
Grand Total
Sum:
298
24841
1077
21678
2618
2025
61
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
5
867
0.43 %
66
7.61 %
766
88.35 %
39
4.50 %
32
3.69 %
3
0.35 %
PAR-CUL-P
5
561
0.28 %
31
5.53 %
474
84.49 %
65
11.59 %
56
9.98 %
0
0.00 %
PAR-HERD-S
1
219
0.11 %
28
12.79 %
186
84.93 %
8
3.65 %
5
2.28 %
0
0.00 %
PAR-OTHER
2
56
0.03 %
3
5.36 %
38
67.86 %
20
35.71 %
15
26.79 %
0
0.00 %
PAR-PERSIS
9
1,166
0.58 %
153
13.12 %
895
76.76 %
131
11.23 %
109
9.35 %
9
0.77 %
SER-ANOM
2
19
0.01 %
0
0.00 %
16
84.21 %
2
10.53 %
2
10.53 %
1
5.26 %
Corn & Scilly
PAR-CUL-N
83
7,426
3.69 %
321
4.32 %
6436
86.67 %
763
10.27 %
656
8.83 %
13
0.18 %
PAR-CUL-P
16
3,527
1.75 %
279
7.91 %
3038
86.14 %
237
6.72 %
208
5.90 %
2
0.06 %
PAR-PERSIS
3
590
0.29 %
30
5.08 %
532
90.17 %
31
5.25 %
28
4.75 %
0
0.00 %
SER-FLEXI
2
264
0.13 %
10
3.79 %
250
94.70 %
6
2.27 %
4
1.52 %
0
0.00 %
Devon
PAR-CUL-N
297
33,252
16.54 %
2124
6.39 %
29153
87.67 %
2220
6.68 %
1829
5.50 %
146
0.44 %
PAR-CUL-P
11
1,404
0.70 %
84
5.98 %
1211
86.25 %
140
9.97 %
108
7.69 %
1
0.07 %
PAR-NEW-IN
11
1,002
0.50 %
63
6.29 %
842
84.03 %
97
9.68 %
94
9.38 %
3
0.30 %
PAR-OTHER
3
58
0.03 %
4
6.90 %
49
84.48 %
6
10.34 %
5
8.62 %
0
0.00 %
PAR-PERSIS
4
628
0.31 %
30
4.78 %
562
89.49 %
41
6.53 %
36
5.73 %
0
0.00 %
SER-FLEXI
1
11
0.01 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
Dorset
PAR-CUL-N
69
8,358
4.16 %
512
6.13 %
7427
88.86 %
529
6.33 %
414
4.95 %
5
0.06 %
PAR-CUL-P
1
64
0.03 %
7
10.94 %
52
81.25 %
5
7.81 %
5
7.81 %
0
0.00 %
PAR-PERSIS
2
405
0.20 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Gloucs
PAR-CUL-N
70
6,929
3.45 %
337
4.86 %
5987
86.40 %
675
9.74 %
568
8.20 %
37
0.53 %
PAR-CUL-P
2
189
0.09 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
PAR-PERSIS
7
1,485
0.74 %
92
6.20 %
1275
85.86 %
178
11.99 %
116
7.81 %
2
0.13 %
Heref
PAR-CUL-N
12
802
0.40 %
46
5.74 %
671
83.67 %
73
9.10 %
66
8.23 %
19
2.37 %
PAR-OTHER
3
620
0.31 %
20
3.23 %
534
86.13 %
69
11.13 %
66
10.65 %
0
0.00 %
PAR-PERSIS
2
110
0.05 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
SER-OTHER
1
52
0.03 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Shropshire
PAR-CUL-N
27
4,557
2.27 %
175
3.84 %
4144
90.94 %
253
5.55 %
220
4.83 %
18
0.39 %
PAR-OTHER
5
523
0.26 %
20
3.82 %
488
93.31 %
17
3.25 %
7
1.34 %
8
1.53 %
Somerset excl N
PAR-CUL-N
81
8,196
4.08 %
588
7.17 %
7279
88.81 %
428
5.22 %
318
3.88 %
11
0.13 %
PAR-CUL-P
9
802
0.40 %
28
3.49 %
731
91.15 %
47
5.86 %
41
5.11 %
2
0.25 %
PAR-NEW-IN
2
370
0.18 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.23 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
5
950
0.47 %
34
3.58 %
879
92.53 %
50
5.26 %
35
3.68 %
2
0.21 %
Staffs
PAR-CUL-N
70
10,587
5.27 %
377
3.56 %
9546
90.17 %
772
7.29 %
662
6.25 %
2
0.02 %
PAR-CUL-P
3
26
0.01 %
2
7.69 %
18
69.23 %
6
23.08 %
6
23.08 %
0
0.00 %
PAR-NEW-IN
3
44
0.02 %
2
4.55 %
36
81.82 %
7
15.91 %
6
13.64 %
0
0.00 %
Wiltshire
PAR-CUL-N
30
3,081
1.53 %
216
7.01 %
2633
85.46 %
271
8.80 %
225
7.30 %
7
0.23 %
PAR-CUL-P
9
851
0.42 %
24
2.82 %
774
90.95 %
45
5.29 %
35
4.11 %
18
2.12 %
PAR-NEW-IN
2
191
0.10 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
10
1,703
0.85 %
76
4.46 %
1535
90.14 %
105
6.17 %
85
4.99 %
7
0.41 %
SER-FLEXI
2
172
0.09 %
13
7.56 %
148
86.05 %
1
0.58 %
1
0.58 %
10
5.81 %
Worcs
PAR-CUL-N
2
128
0.06 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
England - Edge
Berks
PAR-CUL-P
2
319
0.16 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
14
992
0.49 %
61
6.15 %
843
84.98 %
96
9.68 %
88
8.87 %
0
0.00 %
PAR-PERSIS
3
119
0.06 %
8
6.72 %
108
90.76 %
5
4.20 %
3
2.52 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
9
1,360
0.68 %
30
2.21 %
1283
94.34 %
60
4.41 %
46
3.38 %
1
0.07 %
PAR-PERSIS
4
379
0.19 %
7
1.85 %
353
93.14 %
22
5.80 %
19
5.01 %
0
0.00 %
Cheshire
PAR-CUL-N
58
8,816
4.39 %
288
3.27 %
8161
92.57 %
489
5.55 %
366
4.15 %
1
0.01 %
PAR-NEW-IN
30
3,932
1.96 %
118
3.00 %
3577
90.97 %
280
7.12 %
229
5.82 %
8
0.20 %
PAR-PERSIS
4
348
0.17 %
12
3.45 %
316
90.80 %
22
6.32 %
19
5.46 %
1
0.29 %
Derbyshire
PAR-CUL-N
7
985
0.49 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
108
10,518
5.23 %
466
4.43 %
9006
85.62 %
1151
10.94 %
979
9.31 %
67
0.64 %
E Sussex
PAR-NEW-IN
10
806
0.40 %
82
10.17 %
660
81.89 %
54
6.70 %
47
5.83 %
17
2.11 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-CUL-N
3
220
0.11 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
12
1,892
0.94 %
62
3.28 %
xx-xxxx-xxxx
89.43 %
150
7.93 %
136
7.19 %
2
0.11 %
PAR-PERSIS
1
76
0.04 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
28
2,725
1.36 %
128
4.70 %
2429
89.14 %
195
7.16 %
168
6.17 %
0
0.00 %
PAR-PERSIS
3
910
0.45 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
37
2,226
1.11 %
62
2.79 %
2027
91.06 %
173
7.77 %
137
6.15 %
0
0.00 %
PAR-PERSIS
3
184
0.09 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
5
610
0.30 %
27
4.43 %
537
88.03 %
63
10.33 %
45
7.38 %
1
0.16 %
Oxon
PAR-NEW-IN
50
3,744
1.86 %
133
3.55 %
3364
89.85 %
270
7.21 %
243
6.49 %
4
0.11 %
PAR-PERSIS
11
1,852
0.92 %
84
4.54 %
xx-xxxx-xxxx
90.98 %
103
5.56 %
83
4.48 %
0
0.00 %
Warks
PAR-NEW-IN
53
4,177
2.08 %
155
3.71 %
3756
89.92 %
297
7.11 %
256
6.13 %
10
0.24 %
PAR-PERSIS
3
163
0.08 %
10
6.13 %
141
86.50 %
13
7.98 %
12
7.36 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
1
228
0.11 %
2
0.88 %
180
78.95 %
54
23.68 %
43
18.86 %
3
1.32 %
Cleveland
PAR-NEW-IN
2
194
0.10 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
14
2,457
1.22 %
69
2.81 %
2158
87.83 %
249
10.13 %
220
8.95 %
10
0.41 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.05 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
6
540
0.27 %
18
3.33 %
503
93.15 %
24
4.44 %
19
3.52 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.05 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Lancashire
PAR-NEW-IN
7
605
0.30 %
18
2.98 %
549
90.74 %
35
5.79 %
34
5.62 %
4
0.66 %
Lincs
PAR-NEW-IN
15
1,328
0.66 %
42
3.16 %
1176
88.55 %
117
8.81 %
104
7.83 %
6
0.45 %
N Yorks
PAR-NEW-IN
14
526
0.26 %
27
5.13 %
385
73.19 %
121
23.00 %
112
21.29 %
2
0.38 %
Suffolk
PAR-NEW-IN
4
328
0.16 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.10 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
1417
156713
77.97 %
7996
5.10 %
138342
88.28 %
11805
7.53 %
9882
6.31 %
493
0.31 %
Wales - High TB
Carms
PAR-IR-EX
9
48
0.02 %
21
43.75 %
24
50.00 %
2
4.17 %
2
4.17 %
1
2.08 %
PAR-IR-SEV
28
208
0.10 %
53
25.48 %
145
69.71 %
12
5.77 %
10
4.81 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
15
357
0.18 %
46
12.89 %
290
81.23 %
23
6.44 %
19
5.32 %
2
0.56 %
PAR-PERSIS
20
1,309
0.65 %
76
5.81 %
1185
90.53 %
62
4.74 %
48
3.67 %
0
0.00 %
PAR-RAPID
48
143
0.07 %
38
26.57 %
101
70.63 %
4
2.80 %
4
2.80 %
0
0.00 %
PAR-RECUR
5
245
0.12 %
9
3.67 %
225
91.84 %
13
5.31 %
10
4.08 %
1
0.41 %
SER-FLEXI
5
157
0.08 %
23
14.65 %
131
83.44 %
6
3.82 %
3
1.91 %
0
0.00 %
Ceredigion
PAR-IR-EX
4
15
0.01 %
2
13.33 %
13
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
26
189
0.09 %
50
26.46 %
130
68.78 %
12
6.35 %
8
4.23 %
1
0.53 %
PAR-OTHER
2
39
0.02 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
13
1,015
0.50 %
63
6.21 %
894
88.08 %
66
6.50 %
45
4.43 %
13
1.28 %
PAR-RAPID
26
72
0.04 %
19
26.39 %
50
69.44 %
5
6.94 %
3
4.17 %
0
0.00 %
PAR-RECUR
1
5
0.00 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
SER-FLEXI
4
147
0.07 %
13
8.84 %
129
87.76 %
5
3.40 %
4
2.72 %
1
0.68 %
Gwent
PAR-IR-EX
1
15
0.01 %
1
6.67 %
14
93.33 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
23
0.01 %
0
0.00 %
22
95.65 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-OTHER
4
159
0.08 %
9
5.66 %
146
91.82 %
7
4.40 %
4
2.52 %
0
0.00 %
PAR-PERSIS
9
364
0.18 %
24
6.59 %
320
87.91 %
20
5.49 %
19
5.22 %
1
0.27 %
PAR-RAPID
6
20
0.01 %
4
20.00 %
16
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
115
0.06 %
10
8.70 %
100
86.96 %
5
4.35 %
5
4.35 %
0
0.00 %
Pembs
PAR-HERD-S
2
10
0.00 %
1
10.00 %
8
80.00 %
2
20.00 %
1
10.00 %
0
0.00 %
PAR-IR-EX
18
89
0.04 %
15
xx-xxxx-xxxx %
69
77.53 %
6
6.74 %
5
5.62 %
0
0.00 %
PAR-IR-SEV
85
729
0.36 %
180
24.69 %
527
72.29 %
29
3.98 %
22
3.02 %
0
0.00 %
PAR-OTHER
30
1,290
0.64 %
78
6.05 %
1154
89.46 %
66
5.12 %
55
4.26 %
3
0.23 %
PAR-PERSIS
86
4,951
2.46 %
199
4.02 %
4566
92.22 %
242
4.89 %
178
3.60 %
8
0.16 %
PAR-RAPID
96
284
0.14 %
86
30.28 %
189
66.55 %
15
5.28 %
9
3.17 %
0
0.00 %
PAR-RECUR
23
3,376
1.68 %
151
4.47 %
3118
92.36 %
122
3.61 %
96
2.84 %
11
0.33 %
SER-FLEXI
46
1,649
0.82 %
141
8.55 %
1458
88.42 %
67
4.06 %
43
2.61 %
7
0.42 %
Powys
PAR-HERD-S
1
10
0.00 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-IR-EX
10
72
0.04 %
21
29.17 %
47
65.28 %
6
8.33 %
4
5.56 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
17
101
0.05 %
24
23.76 %
75
74.26 %
4
3.96 %
2
1.98 %
0
0.00 %
PAR-NEW-IN
1
239
0.12 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
16
804
0.40 %
41
5.10 %
714
88.81 %
58
7.21 %
43
5.35 %
6
0.75 %
PAR-PERSIS
36
1,920
0.96 %
82
4.27 %
1623
84.53 %
225
11.72 %
207
10.78 %
8
0.42 %
PAR-RAPID
22
44
0.02 %
9
20.45 %
34
77.27 %
2
4.55 %
1
2.27 %
0
0.00 %
PAR-RECUR
4
528
0.26 %
22
4.17 %
479
90.72 %
28
5.30 %
25
4.73 %
2
0.38 %
SER-FLEXI
6
115
0.06 %
17
14.78 %
89
77.39 %
12
10.43 %
9
7.83 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.51 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
2
22
0.01 %
5
22.73 %
17
77.27 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
67
0.03 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.07 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.00 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
8
678
0.34 %
66
9.73 %
582
85.84 %
38
5.60 %
27
3.98 %
3
0.44 %
PAR-PERSIS
6
435
0.22 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
5
7
0.00 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.11 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.04 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-OTHER
3
106
0.05 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.04 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
1
4
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.07 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.07 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.23 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
2
5
0.00 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
22
139
0.07 %
27
19.42 %
104
74.82 %
10
7.19 %
8
5.76 %
0
0.00 %
PAR-NEW-IN
100
8,775
4.37 %
300
3.42 %
8006
91.24 %
571
6.51 %
458
5.22 %
11
0.13 %
PAR-OTHER
5
106
0.05 %
4
3.77 %
95
89.62 %
10
9.43 %
7
6.60 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
17
1,702
0.85 %
46
2.70 %
1583
93.01 %
101
5.93 %
73
4.29 %
0
0.00 %
PAR-POSTM
6
21
0.01 %
0
0.00 %
21
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
12
49
0.02 %
11
22.45 %
37
75.51 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-RECUR
4
205
0.10 %
21
10.24 %
173
84.39 %
12
5.85 %
11
5.37 %
0
0.00 %
SER-FLEXI
14
622
0.31 %
34
5.47 %
573
92.12 %
27
4.34 %
15
2.41 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
19
1,882
0.94 %
53
2.82 %
xx-xxxx-xxxx
89.27 %
163
8.66 %
146
7.76 %
3
0.16 %
PAR-PERSIS
1
14
0.01 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-POSTM
3
5
0.00 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
116
0.06 %
6
5.17 %
99
85.34 %
13
11.21 %
10
8.62 %
1
0.86 %
S Glamorgan
PAR-RAPID
2
7
0.00 %
5
71.43 %
2
28.57 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
221
0.11 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
126
0.06 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
57
0.03 %
1
1.75 %
55
96.49 %
1
1.75 %
1
1.75 %
0
0.00 %
PAR-NEW-IN
26
1,182
0.59 %
41
3.47 %
1053
89.09 %
107
9.05 %
85
7.19 %
3
0.25 %
PAR-OTHER
1
50
0.02 %
0
0.00 %
49
98.00 %
2
4.00 %
1
2.00 %
0
0.00 %
PAR-POSTM
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
61
0.03 %
6
9.84 %
50
81.97 %
6
9.84 %
5
8.20 %
0
0.00 %
NE Wales
PAR-HERD-S
4
111
0.06 %
9
8.11 %
99
89.19 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-NEW-IN
31
2,456
1.22 %
122
4.97 %
2141
87.17 %
223
9.08 %
189
7.70 %
4
0.16 %
PAR-OTHER
4
152
0.08 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.02 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
PAR-POSTM
6
16
0.01 %
0
0.00 %
16
100.00 %
1
6.25 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
607
0.30 %
49
8.07 %
521
85.83 %
43
7.08 %
37
6.10 %
0
0.00 %
Wales
1093
43231
21.51 %
2558
5.92 %
38395
88.81 %
2708
6.26 %
2172
5.02 %
106
0.25 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.09 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
7
846
0.42 %
113
13.36 %
666
78.72 %
65
7.68 %
58
6.86 %
9
1.06 %
Dumfries & G
PAR-NEW-IN
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.01 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
13
1058
0.53 %
129
12.19 %
850
80.34 %
79
7.47 %
69
6.52 %
10
0.95 %
Grand Total
Sum:
xx-xxxx-xxxx
10683
177587
14592
12123
609
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol July 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
170
20366
99.9%
840
4.1%
17661
86.7%
2325
11.4%
1807
8.9%
58
0.3%
Serial/Extended
1
11
0.1%
1
9.1%
9
81.8%
1
9.1%
1
9.1%
0
0.0 %
Total
171
20377
100.0%
841
4.1%
17670
86.7%
2326
11.4%
1808
8.9%
58
0.3%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
119
4050
90.7%
226
5.6%
3619
89.4%
266
6.6%
202
5.0%
3
0.1%
Serial/Extended
8
414
9.3%
10
2.4%
389
94.0%
26
6.3%
15
3.6%
0
0.0 %
Total
127
4464
100.0%
236
5.3%
4008
89.8%
292
6.5%
217
4.9%
3
0.1%
GB
Parallel
289
24416
98.3%
1066
4.4%
21280
87.2%
2591
10.6%
2009
8.2%
61
0.2%
Serial/Extended
9
425
1.7%
11
2.6%
398
93.6%
27
6.4%
16
3.8%
0
0.0 %
Total
298
24841
100.0%
1077
4.3%
21678
87.3%
2618
10.5%
2025
8.2%
61
0.2%
171.00
127.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.7%
7972
5.1% 137868
88.3%
11794
7.6%
9873
6.3%
482
0.3%
Serial/Extended
8
518
0.3%
24
4.6%
474
91.5%
11
2.1%
9
1.7%
11
2.1%
Total
xx-xxxx-xxxx
100.0%
7996
5.1% 138342
88.3%
11805
7.5%
9882
6.3%
493
0.3%
Scotland
Parallel
12
1054
99.6%
129
12.2%
847
80.4%
79
7.5%
69
6.5%
9
0.9%
Serial/Extended
1
4
0.4%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
13
1058
100.0%
129
12.2%
850
80.3%
79
7.5%
69
6.5%
10
0.9%
Wales
Parallel
1003
39640
91.7%
2247
5.7%
35245
88.9%
2533
6.4%
2051
5.2%
97
0.2%
Serial/Extended
90
3591
8.3%
311
8.7%
3150
87.7%
175
4.9%
121
3.4%
9
0.3%
Total
1093
43231
100.0%
2558
5.9%
38395
88.8%
2708
6.3%
2172
5.0%
106
0.2%
GB
Parallel
xx-xxxx-xxxx
98.0% 10348
5.3% 173960
88.4%
14406
7.3%
11993
6.1%
588
0.3%
Serial/Extended
99
4113
2.0%
335
8.1%
3627
88.2%
186
4.5%
130
3.2%
21
0.5%
Total
xx-xxxx-xxxx
100.0% 10683
5.3% 177587
88.4%
14592
7.3%
12123
6.0%
609
0.3%
#######
#######
13.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
425
1.7%
11
2.6%
398
93.6%
27
6.4 %
16
3.8 %
0
Parallel - Herd or Group Slaughter
2
229
0.9%
31
13.5%
190
83.0%
11
4.8 %
8
3.5 %
0
Parallel - IRs in Exit Strategy for persistent her
4
17
0.1%
6
35.3%
11
64.7%
1
5.9 %
0
0
Parallel New Infection
64
4613
18.6%
134
2.9%
3929
85.2%
670
14.5 %
544
11.8 %
6
0.1 %
Parallel - new infection cull area
102
13410
54.0%
476
3.5%
11822
88.2%
1398
10.4 %
1058
7.9 %
54
0.4 %
Parallel - Other
3
106
0.4%
3
2.8%
87
82.1%
22
20.8 %
16
15.1 %
0
Parallel Persistent Infection
32
3854
15.5%
207
5.4%
3411
88.5%
298
7.7 %
235
6.1 %
1
0.0 %
Parallel - persistent infection cull area
11
1741
7.0%
117
6.7%
1499
86.1%
162
9.3 %
125
7.2 %
0
Parallel - Rapid Testing Two-times IRs
21
59
0.2%
17
28.8%
39
66.1%
4
6.8 %
3
5.1 %
0
Parallel - Recurrent breakdowns
3
32
0.1%
0
0.0 %
24
75.0%
8
25.0 %
8
25.0 %
0
Parallel - severe IRs - in persistent herds
34
320
1.3%
74
23.1%
235
73.4%
15
4.7 %
11
3.4 %
0
PAR-POSTM
13
35
0.1%
1
2.9%
33
94.3%
2
5.7 %
1
2.9 %
0
Total
298
24841
100.0%
1077
4.3%
21678
87.3%
2618
10.5 %
2025
8.2 %
61
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (July 2021)
England - HRA
Extended Flexible IFN-GAMMA test
1
11
0.0%
1
9.1%
9
81.8%
1
9.1 %
1
9.1 %
0
Parallel - Herd or Group Slaughter
1
219
0.9%
28
12.8%
186
84.9%
8
3.7 %
5
2.3 %
0
Parallel - new infection cull area
95
11874
47.8%
429
3.6%
10400
87.6%
1310
11.0 %
991
8.3 %
54
0.5 %
Parallel - Other
2
56
0.2%
3
5.4%
38
67.9%
20
35.7 %
15
26.8 %
0
Parallel Persistent Infection
9
1342
5.4%
102
7.6%
1132
84.4%
148
11.0 %
107
8.0 %
1
0.1 %
Parallel - persistent infection cull area
11
1741
7.0%
117
6.7%
1499
86.1%
162
9.3 %
125
7.2 %
0
England - HRA
119
15243
61.4%
680
4.5%
13264
87.0%
xx-xxxx-xxxx
10.8 %
1244
8.2 %
55
0.4 %
England - Edge
Parallel New Infection
35
1956
7.9%
75
3.8%
1625
83.1%
320
16.4 %
256
13.1 %
0
Parallel - new infection cull area
7
1536
6.2%
47
3.1%
1422
92.6%
88
5.7 %
67
4.4 %
0
Parallel Persistent Infection
4
916
3.7%
35
3.8%
837
91.4%
48
5.2 %
44
4.8 %
0
England - Edge
46
4408
17.7%
157
3.6%
3884
88.1%
456
10.3 %
367
8.3 %
0
England - LRA
Parallel New Infection
6
726
2.9%
4
0.6%
522
71.9%
221
30.4 %
197
27.1 %
3
0.4 %
England - LRA
6
726
2.9%
4
0.6%
522
71.9%
221
30.4 %
197
27.1 %
3
0.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
5
227
0.9%
8
3.5%
211
93.0%
15
6.6 %
8
3.5 %
0
Parallel - Herd or Group Slaughter
1
10
0.0%
3
30.0%
4
40.0%
3
30.0 %
3
30.0 %
0
Parallel - IRs in Exit Strategy for persistent her
3
16
0.1%
5
31.3%
11
68.8%
1
6.3 %
0
0
Parallel Persistent Infection
13
986
4.0%
63
6.4%
854
86.6%
76
7.7 %
69
7.0 %
0
Parallel - Rapid Testing Two-times IRs
21
59
0.2%
17
28.8%
39
66.1%
4
6.8 %
3
5.1 %
0
Parallel - Recurrent breakdowns
1
3
0.0%
0
0.0 %
2
66.7%
1
33.3 %
1
33.3 %
0
Parallel - severe IRs - in persistent herds
32
309
1.2%
68
22.0%
230
74.4%
15
4.9 %
11
3.6 %
0
Wales - High TB
76
1610
6.5%
164
10.2%
1351
83.9%
115
7.1 %
95
5.9 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
145
0.6%
1
0.7%
141
97.2%
6
4.1 %
3
2.1 %
0
Parallel - IRs in Exit Strategy for persistent her
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Parallel New Infection
20
1868
7.5%
52
2.8%
1739
93.1%
107
5.7 %
74
4.0 %
3
0.2 %
Parallel Persistent Infection
6
610
2.5%
7
1.1%
588
96.4%
26
4.3 %
15
2.5 %
0
Parallel - Recurrent breakdowns
2
29
0.1%
0
0.0 %
22
75.9%
7
24.1 %
7
24.1 %
0
Parallel - severe IRs - in persistent herds
2
11
0.0%
6
54.5%
5
45.5%
0
0
0
PAR-POSTM
6
16
0.1%
1
6.3%
14
87.5%
1
6.3 %
1
6.3 %
0
Wales - Intermediate TB
39
2680
10.8%
68
2.5%
2509
93.6%
147
5.5 %
100
3.7 %
3
0.1 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
42
0.2%
1
2.4%
37
88.1%
5
11.9 %
4
9.5 %
0
Parallel New Infection
3
63
0.3%
3
4.8%
43
68.3%
22
34.9 %
17
27.0 %
0
Parallel - Other
1
50
0.2%
0
0.0 %
49
98.0%
2
4.0 %
1
2.0 %
0
PAR-POSTM
7
19
0.1%
0
0.0 %
19
100.0%
1
5.3 %
0
0
Wales - Low TB
12
174
0.7%
4
2.3%
148
85.1%
30
17.2 %
22
12.6 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
2
19
0.0%
0
0.0 %
16
84.2%
2
10.5 %
2
10.5 %
1
5.3 %
Extended Flexible IFN-GAMMA test
95
4038
2.0%
335
8.3%
3557
88.1%
183
4.5 %
127
3.1 %
19
0.5 %
Parallel - Herd or Group Slaughter
18
1446
0.7%
87
6.0%
1303
90.1%
63
4.4 %
51
3.5 %
5
0.3 %
Parallel - IRs in Exit Strategy for persistent herds
45
248
0.1%
64
25.8%
172
69.4%
15
6.0 %
11
4.4 %
1
0.4 %
Parallel New Infection
641
57389
28.6%
2342
4.1%
50720
88.4%
4844
8.4 %
4146
7.2 %
181
0.3 %
Parallel - new infection cull area
816
94239
46.9%
5131
5.4%
83353
88.4%
6651
7.1 %
5489
5.8 %
266
0.3 %
Parallel - Other
105
5532
2.8%
326
5.9%
4853
87.7%
382
6.9 %
329
5.9 %
24
0.4 %
Parallel Persistent Infection
268
23030
11.5%
1118
4.9%
20617
89.5%
1565
6.8 %
1242
5.4 %
53
0.2 %
Parallel - persistent infection cull area
58
7743
3.9%
493
6.4%
6738
87.0%
563
7.3 %
471
6.1 %
41
0.5 %
Parallel - Rapid Testing Two-times IRs
224
637
0.3%
178
27.9%
441
69.2%
27
4.2 %
18
2.8 %
0
Parallel - Recurrent breakdowns
49
5169
2.6%
269
5.2%
4700
90.9%
226
4.4 %
184
3.6 %
16
0.3 %
Parallel - severe IRs - in persistent herds
184
1411
0.7%
339
24.0%
1020
72.3%
68
4.8 %
51
3.6 %
1
0.1 %
PAR-POSTM
16
45
0.0%
1
2.2%
43
95.6%
2
4.4 %
1
2.2 %
0
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
xx-xxxx-xxxx
100.0%
10683
5.3%
177587
88.4%
14592
7.3 %
12123
6.0 %
609
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
2
19
0.0%
0
0.0 %
16
84.2%
2
10.5 %
2
10.5 %
1
5.3 %
Extended Flexible IFN-GAMMA test
5
447
0.2%
24
5.4%
407
91.1%
8
1.8 %
6
1.3 %
10
2.2 %
Parallel - Herd or Group Slaughter
1
219
0.1%
28
12.8%
186
84.9%
8
3.7 %
5
2.3 %
0
Parallel New Infection
18
1607
0.8%
131
8.2%
1347
83.8%
132
8.2 %
126
7.8 %
3
0.2 %
Parallel - new infection cull area
746
84183
41.9%
4774
5.7%
74154
88.1%
6026
7.2 %
4992
5.9 %
263
0.3 %
Parallel - Other
15
1724
0.9%
69
4.0%
1498
86.9%
147
8.5 %
149
8.6 %
8
0.5 %
Parallel Persistent Infection
42
7037
3.5%
430
6.1%
6147
87.4%
570
8.1 %
440
6.3 %
20
0.3 %
Parallel - persistent infection cull area
56
7424
3.7%
490
6.6%
6446
86.8%
553
7.4 %
465
6.3 %
23
0.3 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
356
32982
16.4%
1324
4.0%
29174
88.5%
2789
8.5 %
2374
7.2 %
110
0.3 %
Parallel - new infection cull area
69
10038
5.0%
357
3.6%
9193
91.6%
615
6.1 %
487
4.9 %
1
0.0 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel Persistent Infection
32
4031
2.0%
152
3.8%
3676
91.2%
240
6.0 %
201
5.0 %
2
0.0 %
Parallel - persistent infection cull area
2
319
0.2%
3
0.9%
292
91.5%
10
3.1 %
6
1.9 %
18
5.6 %
England - LRA
Parallel New Infection
71
6613
3.3%
214
3.2%
5749
86.9%
694
10.5 %
618
9.3 %
32
0.5 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
xx-xxxx-xxxx
78.0%
7996
5.1%
138342
88.3%
11805
7.5 %
9882
6.3 %
493
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
61
2068
1.0%
194
9.4%
1807
87.4%
90
4.4 %
59
2.9 %
8
0.4 %
Parallel - Herd or Group Slaughter
8
1050
0.5%
48
4.6%
956
91.0%
48
4.6 %
41
3.9 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
42
239
0.1%
60
25.1%
167
69.9%
15
6.3 %
11
4.6 %
1
0.4 %
Parallel New Infection
2
245
0.1%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
69
2716
1.4%
177
6.5%
2396
88.2%
167
6.1 %
132
4.9 %
11
0.4 %
Parallel Persistent Infection
166
9695
4.8%
446
4.6%
8717
89.9%
620
6.4 %
502
5.2 %
30
0.3 %
Parallel - Rapid Testing Two-times IRs
199
565
0.3%
156
27.6%
392
69.4%
26
4.6 %
17
3.0 %
0
Parallel - Recurrent breakdowns
35
4269
2.1%
193
4.5%
3925
91.9%
169
4.0 %
137
3.2 %
14
0.3 %
Parallel - severe IRs - in persistent herds
162
1272
0.6%
312
24.5%
916
72.0%
58
4.6 %
43
3.4 %
1
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
21
855
0.4%
62
7.3%
772
90.3%
36
4.2 %
20
2.3 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
3
9
0.0%
4
44.4%
5
55.6%
0
0
0
Parallel New Infection
125
11250
5.6%
375
3.3%
10191
90.6%
800
7.1 %
665
5.9 %
19
0.2 %
Parallel - Other
16
890
0.4%
76
8.5%
770
86.5%
58
6.5 %
40
4.5 %
4
0.4 %
Parallel Persistent Infection
26
2224
1.1%
88
4.0%
2037
91.6%
135
6.1 %
99
4.5 %
0
Parallel - Rapid Testing Two-times IRs
21
68
0.0%
21
30.9%
46
67.6%
1
1.5 %
1
1.5 %
0
Parallel - Recurrent breakdowns
14
900
0.4%
76
8.4%
775
86.1%
57
6.3 %
47
5.2 %
2
0.2 %
Parallel - severe IRs - in persistent herds
22
139
0.1%
27
19.4%
104
74.8%
10
7.2 %
8
5.8 %
0
PAR-POSTM
9
26
0.0%
1
3.8%
24
92.3%
1
3.8 %
1
3.8 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
8
668
0.3%
55
8.2%
571
85.5%
49
7.3 %
42
6.3 %
0
Parallel - Herd or Group Slaughter
7
168
0.1%
10
6.0%
154
91.7%
6
3.6 %
4
2.4 %
0
Parallel New Infection
57
3638
1.8%
163
4.5%
3194
87.8%
330
9.1 %
274
7.5 %
7
0.2 %
Parallel - Other
5
202
0.1%
4
2.0%
189
93.6%
10
5.0 %
8
4.0 %
1
0.5 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %
Parallel - Rapid Testing Two-times IRs
4
4
0.0%
1
25.0%
3
75.0%
0
0
0
PAR-POSTM
7
19
0.0%
0
0.0 %
19
100.0%
1
5.3 %
0
0
Wales
1093
43231
21.5%
2558
5.9%
38395
88.8%
2708
6.3 %
2172
5.0 %
106
0.2 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Scotland - Scotland
Parallel New Infection
12
1054
0.5%
129
12.2%
847
80.4%
79
7.5 %
69
6.5 %
9
0.9 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
13
1058
0.5%
129
12.2%
850
80.3%
79
7.5 %
69
6.5 %
10
0.9 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
0
65
0
1
October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
3
0
7
1
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
0
84
5
12
March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
0
0
13
3
1338
3
72
1
0
May
357
27182
214
1564
44
65
0
0
0
0
129
0
2
6
4
1178
5
110
1
14
June
355
27828
208
1314
1
24
0
0
0
0
105
0
7
1
0
1078
83
4
0
11
July
298
24841
164
1077
121
531
0
0
0
0
16
0
2
0
0
299
7
0
0
0
Totals (last
12 months)
4120
328470
2444
16118
169
864
0
0
0
0
1178
26
11
79
8
12682
99
840
10
38
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2236
255505
1462
11946
747
0
0
0
0
1040
14
4
47
4
9779
5
93
0
1
Scotland
31
3204
19
187
0
0
0
0
0
47
2
0
6
0
53
13
50
3
12
Wales
1853
69761
963
3985
117
0
0
0
0
91
10
7
26
4
2850
81
697
7
25
Totals
4120
328470
2444
16118
864
0
0
0
0
1178
26
11
79
8
12682
99
840
10
38
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
160
Wiltshire
Warks
S Wales
Staffs
Somerset excl N
Shropshire
Powys
Pembs
Oxon
N Yorks
Notts
Northants
NE Wales
Lincs
Leics & Rut
Heref
Gwynedd
Gwent
Gloucs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Beds
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during July 2021 (% submission per County)
County
%Resampled %Retested
Avon
10.69 %
14.02 %
Beds
18.86 %
23.68 %
Carms
2.97 %
3.47 %
Ceredigion
3.33 %
5.00 %
Cheshire
5.02 %
6.33 %
Corn & Scilly
6.54 %
9.20 %
Cumbria
39.32 %
42.03 %
Derbyshire
13.37 %
17.52 %
Devon
8.94 %
11.74 %
Dorset
7.49 %
10.07 %
Gloucs
9.74 %
12.93 %
Gwent
Gwynedd
13.92 %
18.35 %
Heref
6.45 %
8.39 %
Leics & Rut
20.00 %
20.00 %
Lincs
xx-xxxx-xxxx %
18.56 %
NE Wales
3.46 %
5.34 %
Northants
20.00 %
24.76 %
Notts
10.82 %
13.92 %
N Yorks
66.67 %
77.78 %
Oxon
6.79 %
7.50 %
Pembs
3.48 %
5.57 %
Powys
13.06 %
13.84 %
Shropshire
1.47 %
2.93 %
Somerset excl
6.93 %
9.67 %
Staffs
6.75 %
8.82 %
S Wales
Warks
8.84 %
10.77 %
Wiltshire
9.00 %
10.80 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20066
841
4.2%
20377
841
4.1%
Wales
4393
236
5.4%
4464
236
5.3%
GB
24459
1077
4.4%
24841
1077
4.3%
Table 6a (i): Number of animals and submissions tested in July 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
149758
7993
5.3%
156713
7996
5.1%
Wales
40499
2558
6.3%
43231
2558
5.9%
Scotland
577
129
22.4%
1058
129
12.2%
GB
190824
10680
5.6%
201002
10683
5.3%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of July
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18098
909
5.0%
18301
909
5.0%
Wales
4244
243
5.7%
4289
243
5.7%
Scotland
32
1
3.1%
32
1
3.1%
GB
22374
1153
5.2%
22622
1153
5.1%
Table 6a (ii): Number of animals and submissions tested in July 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of July
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
116936
4906
4.2%
122974
4906
4.0%
Wales
28065
xx-xxxx-xxxx
6.0%
29872
xx-xxxx-xxxx
5.7%
Scotland
3611
103
2.9%
4133
103
2.5%
GB
148611
6698
4.5%
156979
6699
4.3%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Derbyshire
PRV-SP
1
1
0
0
Somerset excl North
PRV-SE
1
49
1
2.04 %
PRV-SP
1
2
0
0
Staffordshire
PRV-SP
1
1
0
0
Worcestershire
PRV-SE
1
1
0
0
Total
5
54
1
1.85 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples July 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
1
1
0
0
Derbyshire
PRV-SP
1
1
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
4
256
6
2.34 %
PRV-SP
2
3
0
0
Staffordshire
PRV-SP
1
1
0
0
Wiltshire
PRV-SE
1
10
0
0
Worcestershire
PRV-SE
1
1
0
0
PRV-SP
1
1
0
0
Total
20
353
10
2.83 %
Isle of Man
SER-IOM
4
4
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
4
60
20
33.3%
BOV-IR-EX
2
9
4
44.4%
Ceredigion
BOV-IR-C
1
5
4
80.0%
North East Wales
BOV-IR-C
3
17
3
17.6%
BOV-IR-EX
1
1
0
0
BOV-P
2
89
8
9.0%
BOV-POSTM
10
28
7
25.0%
Pembrokeshire
BOV-IR-C
14
130
35
26.9%
BOV-IR-EX
1
3
0
0
BOV-OTHER
1
1
0
0
BOV-P
3
311
4
1.3%
Powys
BOV-IR-C
3
28
5
17.9%
South Wales
BOV-IR-C
1
4
0
0
BOV-IR-EX
1
21
1
4.8%
Wales
47
707
91
12.9%
All GB
47
707
91
12.9 %
Table 8a Number of Cattle Antibody Tests July 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
Devon
1
95
6
6.3%
Staffordshire
BOV-P
1
30
1
3.3%
England
3
215
7
3.3%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
21
237
56
23.6%
BOV-IR-EX
6
45
13
28.9%
BOV-OTHER
2
26
2
7.7%
BOV-P
1
26
2
7.7%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
20
165
27
16.4%
BOV-IR-EX
1
8
2
25.0%
BOV-OTHER
6
240
16
6.7%
BOV-P
1
16
0
0
North East Wales
2
24
6
25.0%
BOV-IR-C
18
176
26
14.8%
BOV-IR-EX
2
5
1
20.0%
BOV-OTHER
3
148
4
2.7%
BOV-P
8
884
31
3.5%
BOV-POSTM
11
36
8
22.2%
OTHER
1
48
4
8.3%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
70
712
139
19.5%
BOV-IR-EX
11
67
11
16.4%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-OTHER
16
835
47
5.6%
BOV-P
12
636
27
4.2%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
14
101
18
17.8%
BOV-IR-EX
2
35
7
20.0%
BOV-OTHER
2
107
18
16.8%
BOV-P
3
54
3
5.6%
South Wales
4
14
5
35.7%
BOV-IR-C
6
47
2
4.3%
BOV-IR-EX
1
21
1
4.8%
BOV-P
1
67
0
0
Wales
275
5,120
520
10.2%
All GB
278
5,335
527
9.9 %