Monthly Report: Surveillance Project SB4008 IFNg (TC0651 and TC0751) & Antibody (TC0077) tests for bovine tuberculosis- January  2025
Source: environment.data.gov.uk (2026-01-14)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2025. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.
Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).
Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release.

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period
Issued 10 February 2025
1st - 31st Jan 2025
222

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
January
2025
%
Total 2025
%
Submissions
251
251
Samples
Total (%)
22868
100.00 %
22868
100.00 %
England
15140
66.21 %
15140
66.21 %
Wales
7042
30.79 %
7042
30.79 %
Scotland
686
3.00 %
686
3.00 %
Parallel Tests
PAR-CHR
1216
5.32 %
1216
5.32 %
PAR-CUL-N
2
0.01 %
2
0.01 %
PAR-CUL-P
425
1.86 %
425
1.86 %
PAR-HERD-S
0
0.00 %
PAR-IR-EX
43
0.19 %
43
0.19 %
PAR-IR-SEV
334
1.46 %
334
1.46 %
PAR-NEW-IN
5796
25.35 %
5796
25.35 %
PAR-OTHER
1009
4.41 %
1009
4.41 %
PAR-PERSIS
3878
xx-xxxx-xxxx %
3878
xx-xxxx-xxxx %
PAR-POSTM
0
0.00 %
PAR-RAPID
72
0.31 %
72
0.31 %
PAR-RECUR
9523
41.64 %
9523
41.64 %
Total(% of all samples)
22298
97.51 %
22298
97.51 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
570
2.49 %
570
2.49 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
570
2.49 %
570
2.49 %
Total
22868
100.00 %
22868
100.00 %
Retests
Total (% of all samples)
1390
6.08 %
1390
6.08 %
England
1018
4.45 %
1018
4.45 %
Wales
334
1.46 %
334
1.46 %
Scotland
38
0.17 %
38
0.17 %
Resamples
Total (% of all samples)
1184
5.18 %
1184
5.18 %
England
884
3.87 %
884
3.87 %
Wales
276
1.21 %
276
1.21 %
Scotland
24
0.10 %
24
0.10 %
Rejects
Total (% of all samples)
152
0.66 %
152
0.66 %
England
88
0.38 %
88
0.38 %
Wales
64
0.28 %
64
0.28 %
Scotland
0
0.00 %
Table 1. Monthly Totals for January 2025
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
1216
5.3 %
PAR-CUL-N
2
0.0 %
PAR-CUL-P
425
1.9 %
PAR-IR-EX
43
0.2 %
PAR-IR-SEV
334
1.5 %
PAR-NEW-IN
5796
25.3 %
PAR-OTHER
1009
4.4 %
PAR-PERSIS
3878
17.0 %
PAR-RAPID
72
0.3 %
PAR-RECUR
9523
41.6 %
SER-FLEXI
570
2.5 %
Total
22868
100.00 %
Figure 1. Number of samples submitted in each category during January 2025
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
3500
Dumfries & G
Argyll & Bute
W Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
W Midlands
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Herts
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during January 2025
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
500
1000
1500
2000
2500
3000
3500
Dumfries & G
Argyll & Bute
W Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
W Midlands
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Herts
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2025
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
23
0.10 %
1
4.35 %
18
78.26 %
4
17.39 %
4
17.39 %
0
0.00 %
PAR-RECUR
4
895
3.91 %
58
6.48 %
802
89.61 %
50
5.59 %
35
3.91 %
0
0.00 %
Corn & Scilly
PAR-RECUR
1
5
0.02 %
0
0.00 %
5
100.00 %
5
100.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CHR
2
318
1.39 %
25
7.86 %
287
90.25 %
7
2.20 %
6
1.89 %
0
0.00 %
PAR-CUL-N
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
668
2.92 %
86
12.87 %
559
83.68 %
24
3.59 %
22
3.29 %
1
0.15 %
PAR-RECUR
8
1,713
7.49 %
123
7.18 %
1497
87.39 %
96
5.60 %
88
5.14 %
5
0.29 %
Dorset
PAR-PERSIS
1
279
1.22 %
35
12.54 %
221
79.21 %
26
9.32 %
23
8.24 %
0
0.00 %
PAR-RECUR
1
23
0.10 %
2
8.70 %
10
43.48 %
11
47.83 %
11
47.83 %
0
0.00 %
Gloucs
PAR-RECUR
2
355
1.55 %
27
7.61 %
309
87.04 %
23
6.48 %
19
5.35 %
0
0.00 %
Heref
PAR-CHR
1
11
0.05 %
4
36.36 %
7
63.64 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
306
1.34 %
11
3.59 %
262
85.62 %
35
11.44 %
32
10.46 %
1
0.33 %
Shropshire
PAR-OTHER
3
130
0.57 %
12
9.23 %
108
83.08 %
12
9.23 %
10
7.69 %
0
0.00 %
PAR-RECUR
4
666
2.91 %
48
7.21 %
549
82.43 %
72
10.81 %
68
10.21 %
1
0.15 %
Somerset excl N
PAR-CHR
1
5
0.02 %
2
40.00 %
1
20.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-CUL-P
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-RECUR
8
1,171
5.12 %
21
1.79 %
1042
88.98 %
64
5.47 %
55
4.70 %
53
4.53 %
Staffs
PAR-CHR
3
882
3.86 %
34
3.85 %
772
87.53 %
83
9.41 %
74
8.39 %
2
0.23 %
PAR-RECUR
9
2,242
9.80 %
76
3.39 %
2021
90.14 %
170
7.58 %
142
6.33 %
3
0.13 %
Wiltshire
PAR-CUL-P
1
424
1.85 %
22
5.19 %
389
91.75 %
14
3.30 %
13
3.07 %
0
0.00 %
PAR-RECUR
1
129
0.56 %
14
10.85 %
110
85.27 %
8
6.20 %
5
3.88 %
0
0.00 %
SER-FLEXI
2
31
0.14 %
13
41.94 %
17
54.84 %
1
3.23 %
1
3.23 %
0
0.00 %
W Midlands
PAR-RECUR
2
490
2.14 %
28
5.71 %
441
90.00 %
24
4.90 %
21
4.29 %
0
0.00 %
Worcs
PAR-RECUR
1
115
0.50 %
3
2.61 %
103
89.57 %
10
8.70 %
9
7.83 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
252
1.10 %
6
2.38 %
224
88.89 %
22
8.73 %
22
8.73 %
0
0.00 %
Bucks
PAR-NEW-IN
3
321
1.40 %
31
9.66 %
268
83.49 %
25
7.79 %
22
6.85 %
0
0.00 %
Table 2a. Summary by County for January 2025
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2025 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
17
0.07 %
0
0.00 %
7
41.18 %
10
58.82 %
10
58.82 %
0
0.00 %
Cheshire
PAR-RECUR
2
50
0.22 %
1
2.00 %
45
90.00 %
4
8.00 %
4
8.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
7
312
1.36 %
8
2.56 %
284
91.03 %
24
7.69 %
19
6.09 %
1
0.32 %
PAR-OTHER
2
146
0.64 %
11
7.53 %
130
89.04 %
7
4.79 %
5
3.42 %
0
0.00 %
PAR-RECUR
2
146
0.64 %
3
2.05 %
131
89.73 %
16
10.96 %
12
8.22 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
308
1.35 %
11
3.57 %
293
95.13 %
6
1.95 %
4
1.30 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
223
0.98 %
9
4.04 %
188
84.30 %
29
13.00 %
26
11.66 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
615
2.69 %
29
4.72 %
558
90.73 %
26
4.23 %
24
3.90 %
4
0.65 %
Northants
PAR-NEW-IN
1
134
0.59 %
3
2.24 %
127
94.78 %
5
3.73 %
4
2.99 %
0
0.00 %
Oxon
PAR-RECUR
1
411
1.80 %
19
4.62 %
370
90.02 %
24
5.84 %
21
5.11 %
1
0.24 %
Warks
PAR-RECUR
2
9
0.04 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
1
146
0.64 %
2
1.37 %
132
90.41 %
7
4.79 %
6
4.11 %
6
4.11 %
Cumbria
PAR-NEW-IN
5
515
2.25 %
22
4.27 %
448
86.99 %
40
7.77 %
37
7.18 %
8
1.55 %
Herts
PAR-NEW-IN
1
91
0.40 %
3
3.30 %
84
92.31 %
2
2.20 %
2
2.20 %
2
2.20 %
Lincs
PAR-NEW-IN
5
560
2.45 %
18
3.21 %
517
92.32 %
29
5.18 %
25
4.46 %
0
0.00 %
England
111
15140
66.21 %
823
5.44 %
13345
88.14 %
1018
6.72 %
884
5.84 %
88
0.58 %
Wales - High TB
Carms
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
41
0.18 %
8
19.51 %
32
78.05 %
1
2.44 %
1
2.44 %
0
0.00 %
PAR-OTHER
1
8
0.03 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
8
1,030
4.50 %
52
5.05 %
952
92.43 %
33
3.20 %
24
2.33 %
2
0.19 %
PAR-RAPID
3
15
0.07 %
7
46.67 %
8
53.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
721
3.15 %
56
7.77 %
624
86.55 %
41
5.69 %
39
5.41 %
2
0.28 %
SER-FLEXI
5
484
2.12 %
109
22.52 %
362
74.79 %
19
3.93 %
13
2.69 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
18
0.08 %
7
38.89 %
10
55.56 %
2
11.11 %
1
5.56 %
0
0.00 %
PAR-PERSIS
4
168
0.73 %
18
10.71 %
131
77.98 %
18
10.71 %
17
10.12 %
2
1.19 %
PAR-RAPID
2
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-SEV
1
3
0.01 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2025 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
4
386
1.69 %
36
9.33 %
328
84.97 %
23
5.96 %
22
5.70 %
0
0.00 %
PAR-RAPID
1
6
0.03 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
4
19
0.08 %
10
52.63 %
7
36.84 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-IR-SEV
22
218
0.95 %
104
47.71 %
104
47.71 %
13
5.96 %
10
4.59 %
0
0.00 %
PAR-OTHER
1
17
0.07 %
7
41.18 %
10
58.82 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
5
282
1.23 %
26
9.22 %
240
85.11 %
21
7.45 %
16
5.67 %
0
0.00 %
PAR-RAPID
5
16
0.07 %
3
18.75 %
5
31.25 %
0
0.00 %
0
0.00 %
8
50.00 %
PAR-RECUR
2
6
0.03 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Powys
PAR-IR-EX
1
5
0.02 %
1
20.00 %
2
40.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-IR-SEV
2
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
7
1,548
6.77 %
112
7.24 %
1357
87.66 %
40
2.58 %
34
2.20 %
45
2.91 %
PAR-RAPID
3
11
0.05 %
1
9.09 %
10
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
17
0.07 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
8
0.03 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Ceredigion
PAR-IR-SEV
1
4
0.02 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
181
0.79 %
39
21.55 %
137
75.69 %
6
3.31 %
4
2.21 %
1
0.55 %
PAR-RAPID
2
6
0.03 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
16
0.07 %
6
37.50 %
10
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
47
0.21 %
16
34.04 %
30
63.83 %
1
2.13 %
1
2.13 %
0
0.00 %
PAR-NEW-IN
4
658
2.88 %
32
4.86 %
589
89.51 %
42
6.38 %
34
5.17 %
3
0.46 %
PAR-PERSIS
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
3
542
2.37 %
13
2.40 %
509
93.91 %
31
5.72 %
20
3.69 %
0
0.00 %
PAR-RECUR
2
36
0.16 %
2
5.56 %
30
83.33 %
4
11.11 %
4
11.11 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
9
0.04 %
5
55.56 %
4
44.44 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
5
226
0.99 %
13
5.75 %
197
87.17 %
17
7.52 %
16
7.08 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2025 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
NE Wales
PAR-NEW-IN
4
207
0.91 %
5
2.42 %
190
91.79 %
13
6.28 %
11
5.31 %
1
0.48 %
SER-FLEXI
1
55
0.24 %
13
23.64 %
39
70.91 %
3
5.45 %
3
5.45 %
0
0.00 %
Wales
133
7042
30.79 %
726
10.31 %
5976
84.86 %
334
4.74 %
276
3.92 %
64
0.91 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
159
0.70 %
1
0.63 %
155
97.48 %
6
3.77 %
3
1.89 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
4
527
2.30 %
6
1.14 %
500
94.88 %
32
6.07 %
21
3.98 %
0
0.00 %
Scotland
7
686
3.00 %
7
1.02 %
655
95.48 %
38
5.54 %
24
3.50 %
0
0.00 %
Grand Total
Sum:
251
22868
1556
19976
1390
1184
152
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
23
0.10 %
1
4.35 %
18
78.26 %
4
17.39 %
4
17.39 %
0
0.00 %
PAR-RECUR
4
895
3.91 %
58
6.48 %
802
89.61 %
50
5.59 %
35
3.91 %
0
0.00 %
Corn & Scilly
PAR-RECUR
1
5
0.02 %
0
0.00 %
5
100.00 %
5
100.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CHR
2
318
1.39 %
25
7.86 %
287
90.25 %
7
2.20 %
6
1.89 %
0
0.00 %
PAR-CUL-N
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
668
2.92 %
86
12.87 %
559
83.68 %
24
3.59 %
22
3.29 %
1
0.15 %
PAR-RECUR
8
1,713
7.49 %
123
7.18 %
1497
87.39 %
96
5.60 %
88
5.14 %
5
0.29 %
Dorset
PAR-PERSIS
1
279
1.22 %
35
12.54 %
221
79.21 %
26
9.32 %
23
8.24 %
0
0.00 %
PAR-RECUR
1
23
0.10 %
2
8.70 %
10
43.48 %
11
47.83 %
11
47.83 %
0
0.00 %
Gloucs
PAR-RECUR
2
355
1.55 %
27
7.61 %
309
87.04 %
23
6.48 %
19
5.35 %
0
0.00 %
Heref
PAR-CHR
1
11
0.05 %
4
36.36 %
7
63.64 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
306
1.34 %
11
3.59 %
262
85.62 %
35
11.44 %
32
10.46 %
1
0.33 %
Shropshire
PAR-OTHER
3
130
0.57 %
12
9.23 %
108
83.08 %
12
9.23 %
10
7.69 %
0
0.00 %
PAR-RECUR
4
666
2.91 %
48
7.21 %
549
82.43 %
72
10.81 %
68
10.21 %
1
0.15 %
Somerset excl N
PAR-CHR
1
5
0.02 %
2
40.00 %
1
20.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-CUL-P
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-RECUR
8
1,171
5.12 %
21
1.79 %
1042
88.98 %
64
5.47 %
55
4.70 %
53
4.53 %
Staffs
PAR-CHR
3
882
3.86 %
34
3.85 %
772
87.53 %
83
9.41 %
74
8.39 %
2
0.23 %
PAR-RECUR
9
2,242
9.80 %
76
3.39 %
2021
90.14 %
170
7.58 %
142
6.33 %
3
0.13 %
Wiltshire
PAR-CUL-P
1
424
1.85 %
22
5.19 %
389
91.75 %
14
3.30 %
13
3.07 %
0
0.00 %
PAR-RECUR
1
129
0.56 %
14
10.85 %
110
85.27 %
8
6.20 %
5
3.88 %
0
0.00 %
SER-FLEXI
2
31
0.14 %
13
41.94 %
17
54.84 %
1
3.23 %
1
3.23 %
0
0.00 %
W Midlands
PAR-RECUR
2
490
2.14 %
28
5.71 %
441
90.00 %
24
4.90 %
21
4.29 %
0
0.00 %
Worcs
PAR-RECUR
1
115
0.50 %
3
2.61 %
103
89.57 %
10
8.70 %
9
7.83 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
252
1.10 %
6
2.38 %
224
88.89 %
22
8.73 %
22
8.73 %
0
0.00 %
Bucks
PAR-NEW-IN
3
321
1.40 %
31
9.66 %
268
83.49 %
25
7.79 %
22
6.85 %
0
0.00 %
Table 2b. Summary by County for 2025
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2025 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
17
0.07 %
0
0.00 %
7
41.18 %
10
58.82 %
10
58.82 %
0
0.00 %
Cheshire
PAR-RECUR
2
50
0.22 %
1
2.00 %
45
90.00 %
4
8.00 %
4
8.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
7
312
1.36 %
8
2.56 %
284
91.03 %
24
7.69 %
19
6.09 %
1
0.32 %
PAR-OTHER
2
146
0.64 %
11
7.53 %
130
89.04 %
7
4.79 %
5
3.42 %
0
0.00 %
PAR-RECUR
2
146
0.64 %
3
2.05 %
131
89.73 %
16
10.96 %
12
8.22 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
308
1.35 %
11
3.57 %
293
95.13 %
6
1.95 %
4
1.30 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
223
0.98 %
9
4.04 %
188
84.30 %
29
13.00 %
26
11.66 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
615
2.69 %
29
4.72 %
558
90.73 %
26
4.23 %
24
3.90 %
4
0.65 %
Northants
PAR-NEW-IN
1
134
0.59 %
3
2.24 %
127
94.78 %
5
3.73 %
4
2.99 %
0
0.00 %
Oxon
PAR-RECUR
1
411
1.80 %
19
4.62 %
370
90.02 %
24
5.84 %
21
5.11 %
1
0.24 %
Warks
PAR-RECUR
2
9
0.04 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
1
146
0.64 %
2
1.37 %
132
90.41 %
7
4.79 %
6
4.11 %
6
4.11 %
Cumbria
PAR-NEW-IN
5
515
2.25 %
22
4.27 %
448
86.99 %
40
7.77 %
37
7.18 %
8
1.55 %
Herts
PAR-NEW-IN
1
91
0.40 %
3
3.30 %
84
92.31 %
2
2.20 %
2
2.20 %
2
2.20 %
Lincs
PAR-NEW-IN
5
560
2.45 %
18
3.21 %
517
92.32 %
29
5.18 %
25
4.46 %
0
0.00 %
England
111
15140
66.21 %
823
5.44 %
13345
88.14 %
1018
6.72 %
884
5.84 %
88
0.58 %
Wales - High TB
Carms
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
41
0.18 %
8
19.51 %
32
78.05 %
1
2.44 %
1
2.44 %
0
0.00 %
PAR-OTHER
1
8
0.03 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
8
1,030
4.50 %
52
5.05 %
952
92.43 %
33
3.20 %
24
2.33 %
2
0.19 %
PAR-RAPID
3
15
0.07 %
7
46.67 %
8
53.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
721
3.15 %
56
7.77 %
624
86.55 %
41
5.69 %
39
5.41 %
2
0.28 %
SER-FLEXI
5
484
2.12 %
109
22.52 %
362
74.79 %
19
3.93 %
13
2.69 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
18
0.08 %
7
38.89 %
10
55.56 %
2
11.11 %
1
5.56 %
0
0.00 %
PAR-PERSIS
4
168
0.73 %
18
10.71 %
131
77.98 %
18
10.71 %
17
10.12 %
2
1.19 %
PAR-RAPID
2
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-SEV
1
3
0.01 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2025 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
4
386
1.69 %
36
9.33 %
328
84.97 %
23
5.96 %
22
5.70 %
0
0.00 %
PAR-RAPID
1
6
0.03 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
4
19
0.08 %
10
52.63 %
7
36.84 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-IR-SEV
22
218
0.95 %
104
47.71 %
104
47.71 %
13
5.96 %
10
4.59 %
0
0.00 %
PAR-OTHER
1
17
0.07 %
7
41.18 %
10
58.82 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
5
282
1.23 %
26
9.22 %
240
85.11 %
21
7.45 %
16
5.67 %
0
0.00 %
PAR-RAPID
5
16
0.07 %
3
18.75 %
5
31.25 %
0
0.00 %
0
0.00 %
8
50.00 %
PAR-RECUR
2
6
0.03 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Powys
PAR-IR-EX
1
5
0.02 %
1
20.00 %
2
40.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-IR-SEV
2
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
7
1,548
6.77 %
112
7.24 %
1357
87.66 %
40
2.58 %
34
2.20 %
45
2.91 %
PAR-RAPID
3
11
0.05 %
1
9.09 %
10
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
17
0.07 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
8
0.03 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Ceredigion
PAR-IR-SEV
1
4
0.02 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
181
0.79 %
39
21.55 %
137
75.69 %
6
3.31 %
4
2.21 %
1
0.55 %
PAR-RAPID
2
6
0.03 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
16
0.07 %
6
37.50 %
10
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
47
0.21 %
16
34.04 %
30
63.83 %
1
2.13 %
1
2.13 %
0
0.00 %
PAR-NEW-IN
4
658
2.88 %
32
4.86 %
589
89.51 %
42
6.38 %
34
5.17 %
3
0.46 %
PAR-PERSIS
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
3
542
2.37 %
13
2.40 %
509
93.91 %
31
5.72 %
20
3.69 %
0
0.00 %
PAR-RECUR
2
36
0.16 %
2
5.56 %
30
83.33 %
4
11.11 %
4
11.11 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
9
0.04 %
5
55.56 %
4
44.44 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
5
226
0.99 %
13
5.75 %
197
87.17 %
17
7.52 %
16
7.08 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2025 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
NE Wales
PAR-NEW-IN
4
207
0.91 %
5
2.42 %
190
91.79 %
13
6.28 %
11
5.31 %
1
0.48 %
SER-FLEXI
1
55
0.24 %
13
23.64 %
39
70.91 %
3
5.45 %
3
5.45 %
0
0.00 %
Wales
133
7042
30.79 %
726
10.31 %
5976
84.86 %
334
4.74 %
276
3.92 %
64
0.91 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
159
0.70 %
1
0.63 %
155
97.48 %
6
3.77 %
3
1.89 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
4
527
2.30 %
6
1.14 %
500
94.88 %
32
6.07 %
21
3.98 %
0
0.00 %
Scotland
7
686
3.00 %
7
1.02 %
655
95.48 %
38
5.54 %
24
3.50 %
0
0.00 %
Grand Total
Sum:
251
22868
1556
19976
1390
1184
152
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol January 2025
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
109
15109
99.8%
810
5.4%
13328
88.2%
1017
6.7%
883
5.8%
88
0.6%
Serial/Extended
2
31
0.2%
13
41.9%
17
54.8%
1
3.2%
1
3.2%
0
0.0 %
Total
111
15140
100.0%
823
5.4%
13345
88.1%
1018
6.7%
884
5.8%
88
0.6%
Scotland
Parallel
7
686
100.0%
7
1.0%
655
95.5%
38
5.5%
24
3.5%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
7
686
100.0%
7
1.0%
655
95.5%
38
5.5%
24
3.5%
0
0.0 %
Wales
Parallel
127
6503
92.3%
604
9.3%
5575
85.7%
312
4.8%
260
4.0%
64
1.0%
Serial/Extended
6
539
7.7%
122
22.6%
401
74.4%
22
4.1%
16
3.0%
0
0.0 %
Total
133
7042
100.0%
726
10.3%
5976
84.9%
334
4.7%
276
3.9%
64
0.9%
GB
Parallel
243
22298
97.5%
1421
6.4%
19558
87.7%
1367
6.1%
1167
5.2%
152
0.7%
Serial/Extended
8
570
2.5%
135
23.7%
418
73.3%
23
4.0%
17
3.0%
0
0.0 %
Total
251
22868
100.0%
1556
6.8%
19976
87.4%
1390
6.1%
1184
5.2%
152
0.7%
111.00
133.00
7.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2025)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
109
15109
99.8%
810
5.4%
13328
88.2%
1017
6.7%
883
5.8%
88
0.6%
Serial/Extended
2
31
0.2%
13
41.9%
17
54.8%
1
3.2%
1
3.2%
0
0.0 %
Total
111
15140
100.0%
823
5.4%
13345
88.1%
1018
6.7%
884
5.8%
88
0.6%
Scotland
Parallel
7
686
100.0%
7
1.0%
655
95.5%
38
5.5%
24
3.5%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
7
686
100.0%
7
1.0%
655
95.5%
38
5.5%
24
3.5%
0
0.0 %
Wales
Parallel
127
6503
92.3%
604
9.3%
5575
85.7%
312
4.8%
260
4.0%
64
1.0%
Serial/Extended
6
539
7.7%
122
22.6%
401
74.4%
22
4.1%
16
3.0%
0
0.0 %
Total
133
7042
100.0%
726
10.3%
5976
84.9%
334
4.7%
276
3.9%
64
0.9%
GB
Parallel
243
22298
97.5%
1421
6.4%
19558
87.7%
1367
6.1%
1167
5.2%
152
0.7%
Serial/Extended
8
570
2.5%
135
23.7%
418
73.3%
23
4.0%
17
3.0%
0
0.0 %
Total
251
22868
100.0%
1556
6.8%
19976
87.4%
1390
6.1%
1184
5.2%
152
0.7%
111.00
133.00
7.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
8
570
2.5%
135
23.7%
418
73.3%
23
4.0 %
17
3.0 %
0
Parallel – chronic breakdown
7
1216
5.3%
65
5.3%
1067
87.7%
92
7.6 %
82
6.7 %
2
0.2 %
Parallel - IRs in Exit Strategy for persistent her
9
43
0.2%
20
46.5%
19
44.2%
4
9.3 %
4
9.3 %
0
Parallel New Infection
59
5796
25.3%
212
3.7%
5263
90.8%
356
6.1 %
296
5.1 %
25
0.4 %
Parallel - new infection cull area
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Other
15
1009
4.4%
124
12.3%
843
83.5%
47
4.7 %
41
4.1 %
1
0.1 %
Parallel Persistent Infection
32
3878
17.0%
318
8.2%
3370
86.9%
167
4.3 %
140
3.6 %
50
1.3 %
Parallel - persistent infection cull area
2
425
1.9%
22
5.2%
389
91.5%
15
3.5 %
14
3.3 %
0
Parallel - Rapid Testing Two-times IRs
18
72
0.3%
23
31.9%
41
56.9%
0
0
8
11.1 %
Parallel - Recurrent breakdowns
62
9523
41.6%
495
5.2%
8385
88.0%
669
7.0 %
577
6.1 %
66
0.7 %
Parallel - severe IRs - in persistent herds
38
334
1.5%
142
42.5%
179
53.6%
17
5.1 %
13
3.9 %
0
Total
251
22868
100.0%
1556
6.8%
19976
87.4%
1390
6.1 %
1184
5.2 %
152
0.7 %
Table 4a. Summaryof IFN Gamma results by submission reason (January 2025)
England - HRA
Extended Flexible IFN-GAMMA test
2
31
0.1%
13
41.9%
17
54.8%
1
3.2 %
1
3.2 %
0
Parallel – chronic breakdown
7
1216
5.3%
65
5.3%
1067
87.7%
92
7.6 %
82
6.7 %
2
0.2 %
Parallel - new infection cull area
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Other
8
821
3.6%
99
12.1%
685
83.4%
40
4.9 %
36
4.4 %
1
0.1 %
Parallel Persistent Infection
1
279
1.2%
35
12.5%
221
79.2%
26
9.3 %
23
8.2 %
0
Parallel - persistent infection cull area
2
425
1.9%
22
5.2%
389
91.5%
15
3.5 %
14
3.3 %
0
Parallel - Recurrent breakdowns
44
8110
35.5%
411
5.1%
7151
88.2%
568
7.0 %
485
6.0 %
63
0.8 %
England - HRA
65
10884
47.6%
645
5.9%
9532
87.6%
742
6.8 %
641
5.9 %
66
0.6 %
England - Edge
Parallel New Infection
24
2165
9.5%
97
4.5%
1942
89.7%
137
6.3 %
121
5.6 %
5
0.2 %
Parallel - Other
2
146
0.6%
11
7.5%
130
89.0%
7
4.8 %
5
3.4 %
0
Parallel - Recurrent breakdowns
8
633
2.8%
25
3.9%
560
88.5%
54
8.5 %
47
7.4 %
1
0.2 %
England - Edge
34
2944
12.9%
133
4.5%
2632
89.4%
198
6.7 %
173
5.9 %
6
0.2 %
England - LRA
Parallel New Infection
12
1312
5.7%
45
3.4%
1181
90.0%
78
5.9 %
70
5.3 %
16
1.2 %
England - LRA
12
1312
5.7%
45
3.4%
1181
90.0%
78
5.9 %
70
5.3 %
16
1.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
5
484
2.1%
109
22.5%
362
74.8%
19
3.9 %
13
2.7 %
0
Parallel - IRs in Exit Strategy for persistent her
7
27
0.1%
14
51.9%
9
33.3%
4
14.8 %
4
14.8 %
0
Parallel - Other
4
33
0.1%
9
27.3%
24
72.7%
0
0
0
Parallel Persistent Infection
28
3414
14.9%
244
7.1%
3008
88.1%
135
4.0 %
113
3.3 %
49
1.4 %
Parallel - Rapid Testing Two-times IRs
15
63
0.3%
19
30.2%
36
57.1%
0
0
8
12.7 %
Parallel - Recurrent breakdowns
8
744
3.3%
57
7.7%
644
86.6%
43
5.8 %
41
5.5 %
2
0.3 %
Parallel - severe IRs - in persistent herds
33
283
1.2%
122
43.1%
149
52.7%
16
5.7 %
12
4.2 %
0
Wales - High TB
100
5048
22.1%
574
11.4%
4232
83.8%
217
4.3 %
183
3.6 %
59
1.2 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
2
16
0.1%
6
37.5%
10
62.5%
0
0
0
Parallel New Infection
7
1200
5.2%
45
3.8%
1098
91.5%
73
6.1 %
54
4.5 %
3
0.3 %
Parallel - Other
1
9
0.0%
5
55.6%
4
44.4%
0
0
0
Parallel Persistent Infection
3
185
0.8%
39
21.1%
141
76.2%
6
3.2 %
4
2.2 %
1
0.5 %
Parallel - Rapid Testing Two-times IRs
3
9
0.0%
4
44.4%
5
55.6%
0
0
0
Parallel - Recurrent breakdowns
2
36
0.2%
2
5.6%
30
83.3%
4
11.1 %
4
11.1 %
0
Parallel - severe IRs - in persistent herds
5
51
0.2%
20
39.2%
30
58.8%
1
2.0 %
1
2.0 %
0
Wales - Intermediate TB
23
1506
6.6%
121
8.0%
1318
87.5%
84
5.6 %
63
4.2 %
4
0.3 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
55
0.2%
13
23.6%
39
70.9%
3
5.5 %
3
5.5 %
0
Parallel New Infection
9
433
1.9%
18
4.2%
387
89.4%
30
6.9 %
27
6.2 %
1
0.2 %
Wales - Low TB
10
488
2.1%
31
6.4%
426
87.3%
33
6.8 %
30
6.1 %
1
0.2 %
Scotland - Scotland
Parallel New Infection
7
686
3.0%
7
1.0%
655
95.5%
38
5.5 %
24
3.5 %
0
Scotland - Scotland
7
686
3.0%
7
1.0%
655
95.5%
38
5.5 %
24
3.5 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
8
570
2.5%
135
23.7%
418
73.3%
23
4.0 %
17
3.0 %
0
Parallel – chronic breakdown
7
1216
5.3%
65
5.3%
1067
87.7%
92
7.6 %
82
6.7 %
2
0.2 %
Parallel - IRs in Exit Strategy for persistent herds
9
43
0.2%
20
46.5%
19
44.2%
4
9.3 %
4
9.3 %
0
Parallel New Infection
59
5796
25.3%
212
3.7%
5263
90.8%
356
6.1 %
296
5.1 %
25
0.4 %
Parallel - new infection cull area
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Other
15
1009
4.4%
124
12.3%
843
83.5%
47
4.7 %
41
4.1 %
1
0.1 %
Parallel Persistent Infection
32
3878
17.0%
318
8.2%
3370
86.9%
167
4.3 %
140
3.6 %
50
1.3 %
Parallel - persistent infection cull area
2
425
1.9%
22
5.2%
389
91.5%
15
3.5 %
14
3.3 %
0
Parallel - Rapid Testing Two-times IRs
18
72
0.3%
23
31.9%
41
56.9%
0
0
8
11.1 %
Parallel - Recurrent breakdowns
62
9523
41.6%
495
5.2%
8385
88.0%
669
7.0 %
577
6.1 %
66
0.7 %
Parallel - severe IRs - in persistent herds
38
334
1.5%
142
42.5%
179
53.6%
17
5.1 %
13
3.9 %
0
Total
251
22868
100.0%
1556
6.8%
19976
87.4%
1390
6.1 %
1184
5.2 %
152
0.7 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2025)
England - HRA
Extended Flexible IFN-GAMMA test
2
31
0.1%
13
41.9%
17
54.8%
1
3.2 %
1
3.2 %
0
Parallel – chronic breakdown
7
1216
5.3%
65
5.3%
1067
87.7%
92
7.6 %
82
6.7 %
2
0.2 %
Parallel - new infection cull area
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Other
8
821
3.6%
99
12.1%
685
83.4%
40
4.9 %
36
4.4 %
1
0.1 %
Parallel Persistent Infection
1
279
1.2%
35
12.5%
221
79.2%
26
9.3 %
23
8.2 %
0
Parallel - persistent infection cull area
2
425
1.9%
22
5.2%
389
91.5%
15
3.5 %
14
3.3 %
0
Parallel - Recurrent breakdowns
44
8110
35.5%
411
5.1%
7151
88.2%
568
7.0 %
485
6.0 %
63
0.8 %
England - Edge
Parallel New Infection
24
2165
9.5%
97
4.5%
1942
89.7%
137
6.3 %
121
5.6 %
5
0.2 %
Parallel - Other
2
146
0.6%
11
7.5%
130
89.0%
7
4.8 %
5
3.4 %
0
Parallel - Recurrent breakdowns
8
633
2.8%
25
3.9%
560
88.5%
54
8.5 %
47
7.4 %
1
0.2 %
England - LRA
Parallel New Infection
12
1312
5.7%
45
3.4%
1181
90.0%
78
5.9 %
70
5.3 %
16
1.2 %
England
111
15140
66.2%
823
5.4%
13345
88.1%
1018
6.7 %
884
5.8 %
88
0.6 %
Wales - High TB
Extended Flexible IFN-GAMMA test
5
484
2.1%
109
22.5%
362
74.8%
19
3.9 %
13
2.7 %
0
Parallel - IRs in Exit Strategy for persistent herd
7
27
0.1%
14
51.9%
9
33.3%
4
14.8 %
4
14.8 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2025) Cont.
Parallel - Other
4
33
0.1%
9
27.3%
24
72.7%
0
0
0
Parallel Persistent Infection
28
3414
14.9%
244
7.1%
3008
88.1%
135
4.0 %
113
3.3 %
49
1.4 %
Parallel - Rapid Testing Two-times IRs
15
63
0.3%
19
30.2%
36
57.1%
0
0
8
12.7 %
Parallel - Recurrent breakdowns
8
744
3.3%
57
7.7%
644
86.6%
43
5.8 %
41
5.5 %
2
0.3 %
Parallel - severe IRs - in persistent herds
33
283
1.2%
122
43.1%
149
52.7%
16
5.7 %
12
4.2 %
0
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent herd
2
16
0.1%
6
37.5%
10
62.5%
0
0
0
Parallel New Infection
7
1200
5.2%
45
3.8%
1098
91.5%
73
6.1 %
54
4.5 %
3
0.3 %
Parallel - Other
1
9
0.0%
5
55.6%
4
44.4%
0
0
0
Parallel Persistent Infection
3
185
0.8%
39
21.1%
141
76.2%
6
3.2 %
4
2.2 %
1
0.5 %
Parallel - Rapid Testing Two-times IRs
3
9
0.0%
4
44.4%
5
55.6%
0
0
0
Parallel - Recurrent breakdowns
2
36
0.2%
2
5.6%
30
83.3%
4
11.1 %
4
11.1 %
0
Parallel - severe IRs - in persistent herds
5
51
0.2%
20
39.2%
30
58.8%
1
2.0 %
1
2.0 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
55
0.2%
13
23.6%
39
70.9%
3
5.5 %
3
5.5 %
0
Parallel New Infection
9
433
1.9%
18
4.2%
387
89.4%
30
6.9 %
27
6.2 %
1
0.2 %
Wales
133
7042
30.8%
726
10.3%
5976
84.9%
334
4.7 %
276
3.9 %
64
0.9 %
Scotland - Scotland
Parallel New Infection
7
686
3.0%
7
1.0%
655
95.5%
38
5.5 %
24
3.5 %
0
Scotland
7
686
3.0%
7
1.0%
655
95.5%
38
5.5 %
24
3.5 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2024 February
257
20510
159
1189
13
0
0
0
0
31
1
0
12
0
1021
1
109
1
0
March
215
19876
142
1054
15
0
0
0
0
70
2
0
26
0
879
0
60
0
0
April
237
18969
146
1092
4
0
0
0
0
44
4
0
11
0
958
0
71
0
0
May
207
15411
129
733
0
0
0
0
0
11
3
0
9
0
657
0
53
0
0
June
197
18101
133
797
9
0
0
0
0
32
1
0
19
0
720
0
2
0
14
July
223
19494
124
825
7
0
0
0
0
25
4
0
20
0
753
1
7
1
6
August
197
14635
123
639
25
0
0
0
0
21
2
0
17
0
551
1
21
0
0
September
174
11490
104
803
20
0
0
0
0
88
7
0
8
2
673
0
5
0
0
October
243
20535
159
1136
11
0
0
0
0
35
1
1
26
10
1035
0
19
0
0
November
248
18671
155
1039
2
12
0
0
0
0
79
0
0
12
13
886
0
35
0
0
December
185
14677
117
942
93
0
0
0
0
61
0
18
13
3
739
2
13
0
0
2025 January
251
22868
167
1556
665
350
0
0
0
0
0
0
0
0
0
5
0
0
0
0
Totals (last
12 months)
2634
215237
1658
11805
667
559
0
0
0
0
497
25
19
173
28
8877
5
395
2
20
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1103
140812
743
6475
344
0
0
0
0
412
8
6
95
5
4799
5
155
1
0
Scotland
40
7168
32
174
0
0
0
0
0
0
1
0
15
2
137
0
11
0
0
Wales
1491
67257
883
5156
215
0
0
0
0
85
16
13
63
21
3941
0
229
1
20
Totals
2634
215237
1658
11805
559
0
0
0
0
497
25
19
173
28
8877
5
395
2
20
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
105
176
118
147
107
159
85
118
96
112
95
94
101
108
112
140
109
112
114
139
93
157
69
114
63
171
98
159
73
142
91
146
78
129
64
133
99
124
74
123
70
104
84
159
93
155
68
117
84
167
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2023
2024
2025
IFNg Positive herds
IFNg Negative herds
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
19561
867
17206
638
17659
825
20756
1277
13035
892
20308
1434
18508
1189
18373
1054
17402
1092
14468
733
17182
797
18485
825
13771
639
10556
803
19096
1136
17063
1039
13367
941
20912
1552
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2023
2024
2025
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
10
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2023
2024
2025
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
Worcs
Wiltshire
Warks
Somerset excl N
Powys
Oxon
NE Wales
Leics & Rut
Heref
Gwynedd
Gloucs
Dumfries & G
Devon
Cumbria
Cheshire
Carms
Berks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during January 2025 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
1.89 %
3.77 %
Avon
4.25 %
5.88 %
Beds
4.11 %
4.79 %
Berks
8.73 %
8.73 %
Bucks
9.47 %
10.36 %
Carms
3.35 %
4.09 %
Ceredigion
5.70 %
6.74 %
Cheshire
8.00 %
8.00 %
Corn & Scilly
100.00 %
Cumbria
7.18 %
7.77 %
Derbyshire
5.96 %
7.78 %
Devon
4.29 %
4.70 %
Dorset
11.26 %
12.25 %
Dumfries & G
3.98 %
6.07 %
E Sussex
1.30 %
1.95 %
Gloucs
5.35 %
6.48 %
Gwent
5.56 %
5.81 %
Gwynedd
7.08 %
7.52 %
Hampshire
11.66 %
13.00 %
Heref
10.09 %
11.04 %
Herts
2.20 %
2.20 %
Leics & Rut
3.90 %
4.23 %
Lincs
4.46 %
5.18 %
NE Wales
4.96 %
5.98 %
Northants
2.99 %
3.73 %
Oxon
5.11 %
5.84 %
Pembs
5.20 %
6.63 %
Powys
2.82 %
3.61 %
Shropshire
9.80 %
10.55 %
Somerset excl
4.93 %
5.69 %
Staffs
6.91 %
8.10 %
Warks
W Glamorgan
Wiltshire
3.25 %
3.94 %
W Midlands
4.29 %
4.90 %

Worcs
7.83 %
8.70 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
14848
823
5.5%
15140
823
5.4%
Wales
6952
722
10.4%
7042
726
10.3%
Scotland
665
7
1.1%
686
7
1.0%
GB
22464
1552
6.9%
22868
1556
6.8%
Table 6a (i): Number of animals and submissions tested in January 2025
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
14848
823
5.5%
15140
823
5.4%
Wales
6952
722
10.4%
7042
726
10.3%
Scotland
665
7
1.1%
686
7
1.0%
GB
22464
1552
6.9%
22868
1556
6.8%
Table 6b (i): Number of animals and submissions tested in 2025 Up to end of January
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
11798
687
5.8%
11936
691
5.8%
Wales
9918
747
7.5%
10032
747
7.4%
Scotland
27
0
0.0 %
27
0.0 %
GB
21742
1434
6.6%
21995
1438
6.5%
Table 6a (ii): Number of animals and submissions tested in January 2024
Table 6b (ii): Number of animals and submissions tested in 2024 Up to end of January
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
11798
687
5.8%
11936
691
5.8%
Wales
9918
747
7.5%
10032
747
7.4%
Scotland
27
0
0.0 %
27
0.0 %
GB
21742
1434
6.6%
21995
1438
6.5%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
1
63
16
25.40 %
Total
1
63
16
25.40 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples January 2025
Table 7b Number of private samples 2025
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
1
63
16
25.40 %
Total
1
63
16
25.40 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Wiltshire
BOV-OTHER
1
178
9
5.1%
BOV-P
1
31
1
3.2%
England
2
209
10
4.8%
Wales
Carmarthenshire
BOV-IR-C
8
48
6
12.5%
BOV-IR-EX
1
1
0
0
Ceredigion
BOV-IR-C
2
27
13
48.1%
BOV-IR-EX
1
2
2
100.0%
North East Wales
BOV-IR-C
5
55
11
20.0%
Pembrokeshire
BOV-IR-C
14
174
36
20.7%
BOV-IR-EX
3
18
3
16.7%
Powys
BOV-IR-C
1
1
0
0
BOV-IR-EX
1
5
2
40.0%
South Wales
BOV-IR-C
2
5
2
40.0%
BOV-OTHER
1
14
0
0
Wales
39
350
75
21.4%
All GB
41
559
85
15.2 %
Table 8a Number of Cattle Antibody Tests January 2025
Table 8b Number of Cattle Antibody Tests 2025
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Wiltshire
BOV-OTHER
1
178
9
5.1%
BOV-P
1
31
1
3.2%
England
2
209
10
4.8%
Wales
Carmarthenshire
BOV-IR-C
8
48
6
12.5%
BOV-IR-EX
1
1
0
0
Ceredigion
BOV-IR-C
2
27
13
48.1%
BOV-IR-EX
1
2
2
100.0%
North East Wales
BOV-IR-C
5
55
11
20.0%
Pembrokeshire
BOV-IR-C
14
174
36
20.7%
BOV-IR-EX
3
18
3
16.7%
Powys
BOV-IR-C
1
1
0
0
BOV-IR-EX
1
5
2
40.0%
South Wales
BOV-IR-C
2
5
2
40.0%
BOV-OTHER
1
14
0
0
Wales
39
350
75
21.4%
All GB
41
559
85
15.2 %