Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - November 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 159
Report Period 1st - 30th November 2019
Issued 10 December 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
November
2019
%
Total 2019
%
Submissions
272
3237
Samples
Total (%)
30186
100.00 %
306774
100.00 %
England
23070
76.43 %
244179
79.60 %
Wales
7111
23.56 %
59902
19.53 %
Scotland
5
0.02 %
2693
0.88 %
Parallel Tests
PAR-CUL-N
5414
17.94 %
66128
21.56 %
PAR-CUL-P
3627
12.02 %
21342
6.96 %
PAR-HERD-S
192
0.64 %
7534
2.46 %
PAR-NEW-IN
12820
42.47 %
139614
45.51 %
PAR-OTHER
1042
3.45 %
5216
1.70 %
PAR-PERSIS
6404
21.22 %
59154
19.28 %
PAR-RAPID
135
0.45 %
1385
0.45 %
Total(% of all samples)
29634
98.17 %
300373
97.91 %
Serial/Extended Tests
SER-FLEXI
543
1.80 %
6363
2.07 %
SER-FRAUD
9
0.03 %
27
0.01 %
SER-NSR
0
0.00 %
11
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
552
1.83 %
6401
2.09 %
Total
30186
100.00 %
306774
100.00 %
Retests
Total (% of all samples)
1628
5.39 %
21182
6.90 %
England
1177
3.90 %
17421
5.68 %
Wales
451
1.49 %
3634
1.18 %
Scotland
127
0.04 %
Resamples
Total (% of all samples)
1214
4.02 %
xx-xxxx-xxxx
5.36 %
England
876
2.90 %
13560
4.42 %
Wales
338
1.12 %
2774
0.90 %
Scotland
104
0.03 %
Rejects
Total (% of all samples)
1410
4.67 %
4148
1.35 %
England
1046
3.47 %
3567
1.16 %
Wales
364
1.21 %
571
0.19 %
Scotland
0
0.00 %
10
0.00 %
Table 1. Monthly Totals for November 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-FRAUD
Breakdown of
Reason for
submission
PAR-CUL-N
5414
17.9 %
PAR-CUL-P
3627
12.0 %
PAR-HERD-S
192
0.6 %
PAR-NEW-IN
12820
42.5 %
PAR-OTHER
1042
3.5 %
PAR-PERSIS
6404
21.2 %
PAR-RAPID
135
0.4 %
SER-FLEXI
543
1.8 %
SER-FRAUD
9
0.0 %
Total
30186
100.00 %
Figure 1. Number of samples submitted in each category during November 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
Kirkcudbright
W Glamorgan
Shropshire
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
Suffolk
Somerset excl N
Shropshire
Oxon
Northants
Norfolk
Lincs
Leics & Rut
Heref
Hampshire
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during November 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gr London
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
SER-FRAUD
1
9
0.03 %
6
66.67 %
2
22.22 %
1
11.11 %
1
11.11 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
3
293
0.97 %
10
3.41 %
270
92.15 %
17
5.80 %
13
4.44 %
0
0.00 %
PAR-CUL-P
8
2,040
6.76 %
27
1.32 %
1932
94.71 %
113
5.54 %
79
3.87 %
2
0.10 %
PAR-PERSIS
4
316
1.05 %
10
3.16 %
278
87.97 %
12
3.80 %
28
8.86 %
0
0.00 %
Devon
PAR-CUL-N
19
1,635
5.42 %
61
3.73 %
1357
83.00 %
99
6.06 %
70
4.28 %
147
8.99 %
PAR-CUL-P
2
879
2.91 %
29
3.30 %
805
91.58 %
53
6.03 %
45
5.12 %
0
0.00 %
PAR-PERSIS
3
386
1.28 %
33
8.55 %
336
87.05 %
32
8.29 %
17
4.40 %
0
0.00 %
Dorset
PAR-CUL-N
5
736
2.44 %
20
2.72 %
693
94.16 %
31
4.21 %
23
3.13 %
0
0.00 %
PAR-CUL-P
2
182
0.60 %
4
2.20 %
175
96.15 %
3
1.65 %
3
1.65 %
0
0.00 %
SER-FLEXI
1
231
0.77 %
14
6.06 %
214
92.64 %
4
1.73 %
3
1.30 %
0
0.00 %
Gloucs
PAR-CUL-N
7
169
0.56 %
9
5.33 %
146
86.39 %
13
7.69 %
11
6.51 %
3
1.78 %
Heref
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
92
0.30 %
6
6.52 %
83
90.22 %
5
5.43 %
3
3.26 %
0
0.00 %
Shropshire
PAR-PERSIS
2
385
1.28 %
6
1.56 %
358
92.99 %
27
7.01 %
21
5.45 %
0
0.00 %
Somerset excl N
PAR-CUL-N
2
794
2.63 %
9
1.13 %
282
35.52 %
11
1.39 %
9
1.13 %
494
62.22 %
Wiltshire
PAR-CUL-N
4
376
1.25 %
18
4.79 %
348
92.55 %
13
3.46 %
9
2.39 %
1
0.27 %
PAR-CUL-P
2
526
1.74 %
56
10.65 %
458
87.07 %
35
6.65 %
10
1.90 %
2
0.38 %
PAR-OTHER
2
560
1.86 %
4
0.71 %
548
97.86 %
15
2.68 %
8
1.43 %
0
0.00 %
PAR-PERSIS
5
1,537
5.09 %
78
5.07 %
1299
84.52 %
86
5.60 %
60
3.90 %
100
6.51 %
SER-FLEXI
2
68
0.23 %
9
13.24 %
58
85.29 %
2
2.94 %
1
1.47 %
0
0.00 %
Worcs
PAR-PERSIS
3
97
0.32 %
6
6.19 %
58
59.79 %
2
2.06 %
1
1.03 %
32
32.99 %
England - Edge
Bucks
PAR-NEW-IN
6
258
0.85 %
5
1.94 %
226
87.60 %
9
3.49 %
8
3.10 %
19
7.36 %
Cheshire
PAR-CUL-N
3
1,411
4.67 %
22
1.56 %
1332
94.40 %
73
5.17 %
57
4.04 %
0
0.00 %
PAR-NEW-IN
16
2,991
9.91 %
46
1.54 %
2795
93.45 %
121
4.05 %
83
2.77 %
67
2.24 %
Derbyshire
PAR-NEW-IN
17
2,253
7.46 %
61
2.71 %
2083
92.45 %
133
5.90 %
108
4.79 %
1
0.04 %
Hampshire
PAR-NEW-IN
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2a. Summary by County for November 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
4
347
1.15 %
33
9.51 %
290
83.57 %
29
8.36 %
24
6.92 %
0
0.00 %
Northants
PAR-NEW-IN
3
241
0.80 %
5
2.07 %
154
63.90 %
9
3.73 %
5
2.07 %
77
31.95 %
Oxon
PAR-NEW-IN
20
2,749
9.11 %
113
4.11 %
2420
88.03 %
143
5.20 %
116
4.22 %
100
3.64 %
Warks
PAR-NEW-IN
10
1,149
3.81 %
61
5.31 %
1034
89.99 %
73
6.35 %
53
4.61 %
1
0.09 %
England - LRA
Lincs
PAR-NEW-IN
1
276
0.91 %
9
3.26 %
265
96.01 %
6
2.17 %
2
0.72 %
0
0.00 %
Norfolk
PAR-NEW-IN
1
41
0.14 %
1
2.44 %
38
92.68 %
2
4.88 %
2
4.88 %
0
0.00 %
Suffolk
PAR-NEW-IN
1
36
0.12 %
0
0.00 %
33
91.67 %
5
13.89 %
3
8.33 %
0
0.00 %
England
164
23070
76.43 %
771
3.34 %
20377
88.33 %
1177
5.10 %
876
3.80 %
1046
4.53 %
Wales - High TB
Carms
PAR-HERD-S
4
176
0.58 %
27
15.34 %
135
76.70 %
21
11.93 %
14
7.95 %
0
0.00 %
PAR-PERSIS
1
171
0.57 %
9
5.26 %
159
92.98 %
4
2.34 %
3
1.75 %
0
0.00 %
PAR-RAPID
14
79
0.26 %
19
24.05 %
56
70.89 %
8
10.13 %
4
5.06 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
288
0.95 %
14
4.86 %
262
90.97 %
15
5.21 %
11
3.82 %
1
0.35 %
PAR-RAPID
2
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
21
0.07 %
0
0.00 %
21
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-OTHER
1
6
0.02 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
166
0.55 %
11
6.63 %
150
90.36 %
6
3.61 %
5
3.01 %
0
0.00 %
PAR-RAPID
2
5
0.02 %
1
20.00 %
2
40.00 %
2
40.00 %
2
40.00 %
0
0.00 %
SER-FLEXI
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
2
214
0.71 %
14
6.54 %
188
87.85 %
16
7.48 %
12
5.61 %
0
0.00 %
PAR-PERSIS
13
2,285
7.57 %
93
4.07 %
2043
89.41 %
198
8.67 %
146
6.39 %
3
0.13 %
PAR-RAPID
9
22
0.07 %
5
22.73 %
17
77.27 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
184
0.61 %
21
11.41 %
151
82.07 %
14
7.61 %
12
6.52 %
0
0.00 %
Powys
PAR-HERD-S
2
16
0.05 %
1
6.25 %
10
62.50 %
7
43.75 %
5
31.25 %
0
0.00 %
PAR-NEW-IN
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.04 %
3
27.27 %
6
54.55 %
3
27.27 %
2
18.18 %
0
0.00 %
PAR-PERSIS
4
341
1.13 %
9
2.64 %
123
36.07 %
12
3.52 %
9
2.64 %
200
58.65 %
PAR-RAPID
5
15
0.05 %
1
6.67 %
13
86.67 %
1
6.67 %
1
6.67 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Glamorgan
PAR-OTHER
1
13
0.04 %
4
30.77 %
9
69.23 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-PERSIS
2
282
0.93 %
19
6.74 %
252
89.36 %
17
6.03 %
11
3.90 %
0
0.00 %
PAR-RAPID
3
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
11
1,963
6.50 %
34
1.73 %
1717
87.47 %
84
4.28 %
69
3.52 %
143
7.28 %
PAR-OTHER
3
238
0.79 %
5
2.10 %
223
93.70 %
10
4.20 %
10
4.20 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
34
0.11 %
1
2.94 %
16
47.06 %
0
0.00 %
0
0.00 %
17
50.00 %
Powys
PAR-NEW-IN
2
489
1.62 %
10
2.04 %
468
95.71 %
19
3.89 %
11
2.25 %
0
0.00 %
PAR-PERSIS
2
21
0.07 %
2
9.52 %
17
80.95 %
2
9.52 %
2
9.52 %
0
0.00 %
Shropshire
PAR-PERSIS
1
9
0.03 %
1
11.11 %
6
66.67 %
3
33.33 %
2
22.22 %
0
0.00 %
W Glamorgan
PAR-PERSIS
2
28
0.09 %
2
7.14 %
21
75.00 %
7
25.00 %
5
17.86 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
1
11
0.04 %
0
0.00 %
9
81.82 %
2
18.18 %
2
18.18 %
0
0.00 %
Wales
107
7111
23.56 %
309
4.35 %
6100
85.78 %
451
6.34 %
338
4.75 %
364
5.12 %
Scotland - Scotland
Kirkcudbright
PAR-NEW-IN
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Scotland
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Grand Total
Sum:
272
30186
1080
26482
1628
1214
1410
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
3
431
0.14 %
9
2.09 %
407
94.43 %
23
5.34 %
15
3.48 %
0
0.00 %
PAR-OTHER
4
83
0.03 %
1
1.20 %
70
84.34 %
12
14.46 %
12
14.46 %
0
0.00 %
PAR-PERSIS
9
1,490
0.49 %
45
3.02 %
1387
93.09 %
72
4.83 %
56
3.76 %
2
0.13 %
SER-FRAUD
3
27
0.01 %
6
22.22 %
19
70.37 %
2
7.41 %
2
7.41 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
47
4,595
1.50 %
151
3.29 %
4109
89.42 %
352
7.66 %
284
6.18 %
51
1.11 %
PAR-CUL-P
12
3,205
1.04 %
46
1.44 %
2967
92.57 %
245
7.64 %
188
5.87 %
4
0.12 %
PAR-NEW-IN
7
793
0.26 %
14
1.77 %
699
88.15 %
103
12.99 %
80
10.09 %
0
0.00 %
PAR-PERSIS
37
8,245
2.69 %
337
4.09 %
7250
87.93 %
452
5.48 %
363
4.40 %
295
3.58 %
SER-FLEXI
4
43
0.01 %
0
0.00 %
41
95.35 %
2
4.65 %
2
4.65 %
0
0.00 %
Devon
PAR-CUL-N
218
28,226
9.20 %
1357
4.81 %
25004
88.58 %
1944
6.89 %
1455
5.15 %
410
1.45 %
PAR-CUL-P
30
7,658
2.50 %
342
4.47 %
6899
90.09 %
514
6.71 %
413
5.39 %
4
0.05 %
PAR-NEW-IN
4
674
0.22 %
106
15.73 %
501
74.33 %
86
12.76 %
67
9.94 %
0
0.00 %
PAR-OTHER
7
532
0.17 %
6
1.13 %
352
66.17 %
202
37.97 %
174
32.71 %
0
0.00 %
PAR-PERSIS
25
3,240
1.06 %
214
6.60 %
2828
87.28 %
263
8.12 %
197
6.08 %
1
0.03 %
SER-FLEXI
8
849
0.28 %
50
5.89 %
732
86.22 %
47
5.54 %
22
2.59 %
45
5.30 %
Dorset
PAR-CUL-N
99
12,774
4.16 %
652
5.10 %
11391
89.17 %
880
6.89 %
675
5.28 %
56
0.44 %
PAR-CUL-P
19
3,389
1.10 %
254
7.49 %
3018
89.05 %
159
4.69 %
117
3.45 %
0
0.00 %
PAR-NEW-IN
5
55
0.02 %
1
1.82 %
43
78.18 %
11
20.00 %
11
20.00 %
0
0.00 %
SER-FLEXI
5
384
0.13 %
20
5.21 %
351
91.41 %
15
3.91 %
13
3.39 %
0
0.00 %
Gloucs
PAR-CUL-N
59
4,344
1.42 %
165
3.80 %
3734
85.96 %
398
9.16 %
333
7.67 %
112
2.58 %
PAR-CUL-P
10
1,278
0.42 %
52
4.07 %
1115
87.25 %
140
10.95 %
111
8.69 %
0
0.00 %
PAR-HERD-S
4
33
0.01 %
0
0.00 %
31
93.94 %
3
9.09 %
2
6.06 %
0
0.00 %
PAR-NEW-IN
1
112
0.04 %
3
2.68 %
103
91.96 %
7
6.25 %
6
5.36 %
0
0.00 %
SER-FLEXI
2
30
0.01 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
15
1,724
0.56 %
69
4.00 %
1541
89.39 %
129
7.48 %
103
5.97 %
11
0.64 %
PAR-CUL-P
4
418
0.14 %
16
3.83 %
373
89.23 %
36
8.61 %
29
6.94 %
0
0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
2
13
0.00 %
0
0.00 %
12
92.31 %
4
30.77 %
1
7.69 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
33
3,504
1.14 %
144
4.11 %
3145
89.75 %
257
7.33 %
203
5.79 %
12
0.34 %
Shropshire
PAR-CUL-P
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
7
635
0.21 %
43
6.77 %
545
85.83 %
60
9.45 %
46
7.24 %
1
0.16 %
PAR-PERSIS
12
1,320
0.43 %
32
2.42 %
1167
88.41 %
138
10.45 %
120
9.09 %
1
0.08 %
SER-FLEXI
5
557
0.18 %
21
3.77 %
509
91.38 %
34
6.10 %
27
4.85 %
0
0.00 %
Somerset excl N
PAR-CUL-N
48
5,658
1.84 %
229
4.05 %
4657
82.31 %
363
6.42 %
241
4.26 %
531
9.38 %
PAR-OTHER
2
238
0.08 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
13
1,907
0.62 %
110
5.77 %
1510
79.18 %
105
5.51 %
69
3.62 %
218
11.43 %
Staffs
PAR-OTHER
2
140
0.05 %
3
2.14 %
125
89.29 %
15
10.71 %
12
8.57 %
0
0.00 %
PAR-PERSIS
7
1,492
0.49 %
202
13.54 %
1201
80.50 %
130
8.71 %
90
6.03 %
0
0.00 %
Wiltshire
PAR-CUL-N
69
7,098
2.31 %
251
3.54 %
6368
89.72 %
527
7.42 %
446
6.28 %
33
0.46 %
PAR-CUL-P
26
4,602
1.50 %
296
6.43 %
4112
89.35 %
283
6.15 %
191
4.15 %
3
0.07 %
PAR-NEW-IN
7
456
0.15 %
15
3.29 %
398
87.28 %
53
11.62 %
43
9.43 %
0
0.00 %
PAR-OTHER
4
597
0.19 %
5
0.84 %
582
97.49 %
19
3.18 %
10
1.68 %
0
0.00 %
PAR-PERSIS
15
3,575
1.17 %
160
4.48 %
3174
88.78 %
195
5.45 %
129
3.61 %
112
3.13 %
SER-FLEXI
3
70
0.02 %
9
12.86 %
59
84.29 %
3
4.29 %
2
2.86 %
0
0.00 %
Worcs
PAR-PERSIS
4
138
0.04 %
6
4.35 %
99
71.74 %
2
1.45 %
1
0.72 %
32
23.19 %
England - Edge
Berks
PAR-NEW-IN
39
4,468
1.46 %
155
3.47 %
4016
89.88 %
362
8.10 %
297
6.65 %
0
0.00 %
PAR-PERSIS
3
204
0.07 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
34
3,052
0.99 %
99
3.24 %
2698
88.40 %
270
8.85 %
220
7.21 %
35
1.15 %
Cheshire
PAR-CUL-N
5
1,709
0.56 %
43
2.52 %
1590
93.04 %
94
5.50 %
76
4.45 %
0
0.00 %
PAR-NEW-IN
225
46,214
15.06 %
1017
2.20 %
42592
92.16 %
2670
5.78 %
1963
4.25 %
642
1.39 %
PAR-PERSIS
5
540
0.18 %
3
0.56 %
512
94.81 %
29
5.37 %
25
4.63 %
0
0.00 %
Derbyshire
PAR-NEW-IN
174
19,542
6.37 %
610
3.12 %
17379
88.93 %
1526
7.81 %
1263
6.46 %
290
1.48 %
E Sussex
PAR-NEW-IN
21
2,721
0.89 %
125
4.59 %
2450
90.04 %
157
5.77 %
149
5.48 %
13
0.48 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-NEW-IN
37
3,029
0.99 %
71
2.34 %
2744
90.59 %
270
8.91 %
216
7.13 %
0
0.00 %
PAR-PERSIS
1
37
0.01 %
2
5.41 %
28
75.68 %
9
24.32 %
7
18.92 %
0
0.00 %
SER-NSR
2
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
58
6,625
2.16 %
294
4.44 %
5862
88.48 %
463
6.99 %
374
5.65 %
95
1.43 %
PAR-PERSIS
1
336
0.11 %
20
5.95 %
305
90.77 %
11
3.27 %
8
2.38 %
3
0.89 %
SER-NSR
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
31
2,222
0.72 %
123
5.54 %
1932
86.95 %
141
6.35 %
90
4.05 %
77
3.47 %
Notts
PAR-NEW-IN
7
774
0.25 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
154
15,337
5.00 %
611
3.98 %
13578
88.53 %
1253
8.17 %
993
6.47 %
155
1.01 %
PAR-PERSIS
2
339
0.11 %
5
1.47 %
312
92.04 %
28
8.26 %
22
6.49 %
0
0.00 %
Warks
PAR-NEW-IN
108
9,121
2.97 %
396
4.34 %
8121
89.04 %
658
7.21 %
517
5.67 %
87
0.95 %
PAR-PERSIS
6
570
0.19 %
19
3.33 %
526
92.28 %
32
5.61 %
25
4.39 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
7
409
0.13 %
22
5.38 %
355
86.80 %
30
7.33 %
24
5.87 %
8
1.96 %
Cumbria
PAR-NEW-IN
21
3,783
1.23 %
110
2.91 %
3430
90.67 %
325
8.59 %
243
6.42 %
0
0.00 %
PAR-PERSIS
1
135
0.04 %
4
2.96 %
121
89.63 %
19
14.07 %
10
7.41 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.03 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr London
PAR-NEW-IN
2
45
0.01 %
0
0.00 %
42
93.33 %
6
13.33 %
3
6.67 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
79
0.03 %
6
7.59 %
52
65.82 %
25
31.65 %
21
26.58 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.33 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
4
641
0.21 %
16
2.50 %
562
87.68 %
69
10.76 %
62
9.67 %
1
0.16 %
Kent
PAR-NEW-IN
1
10
0.00 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
106
0.03 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
12
1,350
0.44 %
33
2.44 %
1202
89.04 %
128
9.48 %
115
8.52 %
0
0.00 %
Norfolk
PAR-NEW-IN
1
41
0.01 %
1
2.44 %
38
92.68 %
2
4.88 %
2
4.88 %
0
0.00 %
Northumb
PAR-NEW-IN
3
296
0.10 %
0
0.00 %
241
81.42 %
63
21.28 %
55
18.58 %
0
0.00 %
PAR-PERSIS
3
183
0.06 %
1
0.55 %
32
17.49 %
58
31.69 %
57
31.15 %
93
50.82 %
N Yorks
PAR-NEW-IN
7
875
0.29 %
22
2.51 %
766
87.54 %
71
8.11 %
59
6.74 %
28
3.20 %
PAR-PERSIS
3
575
0.19 %
2
0.35 %
551
95.83 %
31
5.39 %
22
3.83 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
2
50
0.02 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
Suffolk
PAR-NEW-IN
3
334
0.11 %
3
0.90 %
291
87.13 %
45
13.47 %
40
11.98 %
0
0.00 %
S Yorks
PAR-NEW-IN
5
404
0.13 %
7
1.73 %
381
94.31 %
21
5.20 %
15
3.71 %
1
0.25 %
W Sussex
PAR-NEW-IN
2
239
0.08 %
2
0.84 %
163
68.20 %
81
33.89 %
74
30.96 %
0
0.00 %
W Yorks
PAR-HERD-S
2
24
0.01 %
8
33.33 %
14
58.33 %
2
8.33 %
2
8.33 %
0
0.00 %
England
1911
244179
79.60 %
9403
3.85 %
217668
89.14 %
17421
7.13 %
13560
5.55 %
3567
1.46 %
Wales - High TB
Carms
PAR-HERD-S
59
3,326
1.08 %
234
7.04 %
2909
87.46 %
237
7.13 %
181
5.44 %
2
0.06 %
PAR-NEW-IN
3
121
0.04 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
16
464
0.15 %
70
15.09 %
384
82.76 %
20
4.31 %
10
2.16 %
0
0.00 %
PAR-PERSIS
73
6,610
2.15 %
359
5.43 %
5995
90.70 %
331
5.01 %
245
3.71 %
13
0.20 %
PAR-RAPID
82
365
0.12 %
91
24.93 %
256
70.14 %
31
8.49 %
18
4.93 %
0
0.00 %
SER-FLEXI
43
908
0.30 %
92
10.13 %
775
85.35 %
51
5.62 %
41
4.52 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.05 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-NEW-IN
2
313
0.10 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
3
160
0.05 %
5
3.13 %
148
92.50 %
10
6.25 %
7
4.38 %
0
0.00 %
PAR-PERSIS
35
2,567
0.84 %
153
5.96 %
2275
88.62 %
184
7.17 %
138
5.38 %
1
0.04 %
PAR-RAPID
51
129
0.04 %
26
20.16 %
99
76.74 %
6
4.65 %
4
3.10 %
0
0.00 %
SER-FLEXI
10
533
0.17 %
18
3.38 %
501
94.00 %
15
2.81 %
12
2.25 %
2
0.38 %
Gwent
PAR-HERD-S
11
887
0.29 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
3
30
0.01 %
6
20.00 %
24
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
20
1,411
0.46 %
76
5.39 %
1271
90.08 %
84
5.95 %
63
4.46 %
1
0.07 %
PAR-RAPID
28
83
0.03 %
19
22.89 %
55
66.27 %
9
10.84 %
8
9.64 %
1
1.20 %
SER-FLEXI
2
24
0.01 %
2
8.33 %
22
91.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
36
2,381
0.78 %
79
3.32 %
2241
94.12 %
107
4.49 %
59
2.48 %
3
0.13 %
PAR-NEW-IN
3
8
0.00 %
2
25.00 %
4
50.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-OTHER
19
1,437
0.47 %
71
4.94 %
1269
88.31 %
122
8.49 %
97
6.75 %
0
0.00 %
PAR-PERSIS
135
13,900
4.53 %
460
3.31 %
12706
91.41 %
922
6.63 %
726
5.22 %
8
0.06 %
PAR-RAPID
126
339
0.11 %
63
18.58 %
250
73.75 %
20
5.90 %
13
3.83 %
13
3.83 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
47
1,185
0.39 %
84
7.09 %
1051
88.69 %
67
5.65 %
49
4.14 %
1
0.08 %
Powys
PAR-HERD-S
26
518
0.17 %
55
10.62 %
433
83.59 %
37
7.14 %
26
5.02 %
4
0.77 %
PAR-NEW-IN
30
981
0.32 %
43
4.38 %
901
91.85 %
44
4.49 %
36
3.67 %
1
0.10 %
PAR-OTHER
2
15
0.00 %
4
26.67 %
9
60.00 %
3
20.00 %
2
13.33 %
0
0.00 %
PAR-PERSIS
42
2,381
0.78 %
88
3.70 %
1974
82.91 %
135
5.67 %
109
4.58 %
210
8.82 %
PAR-RAPID
64
183
0.06 %
24
13.11 %
121
66.12 %
14
7.65 %
13
7.10 %
25
13.66 %
SER-FLEXI
14
388
0.13 %
25
6.44 %
356
91.75 %
10
2.58 %
7
1.80 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.00 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.13 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.03 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.04 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-OTHER
1
13
0.00 %
4
30.77 %
9
69.23 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
7
0.00 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
75
0.02 %
14
18.67 %
59
78.67 %
3
4.00 %
2
2.67 %
0
0.00 %
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.11 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
9
842
0.27 %
48
5.70 %
768
91.21 %
44
5.23 %
26
3.09 %
0
0.00 %
PAR-RAPID
14
28
0.01 %
3
10.71 %
24
85.71 %
1
3.57 %
1
3.57 %
0
0.00 %
SER-FLEXI
1
20
0.01 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.00 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
2
148
0.05 %
21
14.19 %
111
75.00 %
17
11.49 %
16
10.81 %
0
0.00 %
PAR-PERSIS
4
550
0.18 %
26
4.73 %
500
90.91 %
28
5.09 %
24
4.36 %
0
0.00 %
PAR-RAPID
5
14
0.00 %
8
57.14 %
6
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
27
0.01 %
2
7.41 %
21
77.78 %
4
14.81 %
4
14.81 %
0
0.00 %
NE Wales
PAR-NEW-IN
86
8,270
2.70 %
230
2.78 %
7477
90.41 %
483
5.84 %
365
4.41 %
198
2.39 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
13
323
0.11 %
21
6.50 %
284
87.93 %
21
6.50 %
18
5.57 %
0
0.00 %
PAR-PERSIS
10
1,617
0.53 %
39
2.41 %
1528
94.50 %
87
5.38 %
59
3.65 %
0
0.00 %
PAR-RAPID
16
180
0.06 %
9
5.00 %
165
91.67 %
6
3.33 %
6
3.33 %
0
0.00 %
SER-FLEXI
43
1,046
0.34 %
73
6.98 %
860
82.22 %
53
5.07 %
43
4.11 %
70
6.69 %
Powys
PAR-NEW-IN
13
1,548
0.50 %
37
2.39 %
1432
92.51 %
95
6.14 %
79
5.10 %
0
0.00 %
PAR-PERSIS
2
21
0.01 %
2
9.52 %
17
80.95 %
2
9.52 %
2
9.52 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
9
42
0.01 %
5
11.90 %
37
88.10 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
59
0.02 %
10
xx-xxxx-xxxx %
48
81.36 %
2
3.39 %
1
1.69 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
165
0.05 %
10
6.06 %
144
87.27 %
13
7.88 %
11
6.67 %
0
0.00 %
PAR-PERSIS
1
9
0.00 %
1
11.11 %
6
66.67 %
3
33.33 %
2
22.22 %
0
0.00 %
S Wales
PAR-RAPID
2
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.00 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
258
0.08 %
17
6.59 %
220
85.27 %
29
11.24 %
21
8.14 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
17
816
0.27 %
22
2.70 %
750
91.91 %
64
7.84 %
47
5.76 %
1
0.12 %
PAR-OTHER
1
17
0.01 %
0
0.00 %
17
100.00 %
1
5.88 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
14
460
0.15 %
8
1.74 %
421
91.52 %
25
5.43 %
16
3.48 %
15
3.26 %
PAR-OTHER
2
18
0.01 %
1
5.56 %
15
83.33 %
3
16.67 %
2
11.11 %
0
0.00 %
PAR-PERSIS
2
429
0.14 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
89
0.03 %
4
4.49 %
82
92.13 %
4
4.49 %
3
3.37 %
0
0.00 %
Wales
1304
59902
19.53 %
2917
4.87 %
53656
89.57 %
3634
6.07 %
2774
4.63 %
571
0.95 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.01 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
366
0.12 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
5
757
0.25 %
10
1.32 %
708
93.53 %
36
4.76 %
29
3.83 %
10
1.32 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Kirkcudbright
PAR-CUL-P
1
352
0.11 %
7
1.99 %
340
96.59 %
8
2.27 %
5
1.42 %
0
0.00 %
PAR-NEW-IN
6
421
0.14 %
55
13.06 %
348
82.66 %
20
4.75 %
18
4.28 %
0
0.00 %
PAR-PERSIS
1
338
0.11 %
2
0.59 %
329
97.34 %
7
2.07 %
7
2.07 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
420
0.14 %
11
2.62 %
381
90.71 %
36
8.57 %
28
6.67 %
0
0.00 %
Scotland
22
2693
0.88 %
90
3.34 %
2489
92.42 %
127
4.72 %
104
3.86 %
10
0.37 %
Grand Total
Sum:
xx-xxxx-xxxx
12410
273813
21182
xx-xxxx-xxxx
4148
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol November 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
160
22762
98.7%
742
3.3%
20103
88.3%
1170
5.1%
871
3.8%
1046
4.6%
Serial/Extended
4
308
1.3%
29
9.4%
274
89.0%
7
2.3%
5
1.6%
0
0.0 %
Total
164
23070
100.0%
771
3.3%
20377
88.3%
1177
5.1%
876
3.8%
1046
4.5%
Scotland
Parallel
1
5
100.0%
0
0.0 %
5 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
5
100.0%
0
0.0 %
5 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
97
6867
96.6%
287
4.2%
5907
86.0%
437
6.4%
326
4.7%
347
5.1%
Serial/Extended
10
244
3.4%
22
9.0%
193
79.1%
14
5.7%
12
4.9%
17
7.0%
Total
107
7111
100.0%
309
4.3%
6100
85.8%
451
6.3%
338
4.8%
364
5.1%
GB
Parallel
258
29634
98.2%
1029
3.5%
26015
87.8%
1607
5.4%
1197
4.0%
1393
4.7%
Serial/Extended
14
552
1.8%
51
9.2%
467
84.6%
21
3.8%
17
3.1%
17
3.1%
Total
272
30186
100.0%
1080
3.6%
26482
87.7%
1628
5.4%
1214
4.0%
1410
4.7%
xx-xxxx-xxxx
107.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.2%
9294
3.8% 215870
89.1%
17313
7.1%
13491
5.6%
3522
1.5%
Serial/Extended
35
2021
0.8%
109
5.4%
1798
89.0%
108
5.3%
69
3.4%
45
2.2%
Total
1911 244179
100.0%
9403
3.9% 217668
89.1%
17421
7.1%
13560
5.6%
3567
1.5%
Scotland
Parallel
22
2693
100.0%
90
3.3%
2489
92.4%
127
4.7%
104
3.9%
10
0.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
22
2693
100.0%
90
3.3%
2489
92.4%
127
4.7%
104
3.9%
10
0.4%
Wales
Parallel
1134
55522
92.7%
2590
4.7%
49840
89.8%
3423
6.2%
2610
4.7%
498
0.9%
Serial/Extended
170
4380
7.3%
327
7.5%
3816
87.1%
211
4.8%
164
3.7%
73
1.7%
Total
1304
59902
100.0%
2917
4.9%
53656
89.6%
3634
6.1%
2774
4.6%
571
1.0%
GB
Parallel
xx-xxxx-xxxx
97.9% 11974
4.0% 268199
89.3%
20863
6.9%
16205
5.4%
4030
1.3%
Serial/Extended
205
6401
2.1%
436
6.8%
5614
87.7%
319
5.0%
233
3.6%
118
1.8%
Total
xx-xxxx-xxxx
100.0% 12410
4.0% 273813
89.3%
21182
6.9%
xx-xxxx-xxxx
5.4%
4148
1.4%
#######
#######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
543
1.8%
45
8.3%
465
85.6%
20
3.7 %
16
2.9 %
17
3.1 %
Parallel - Herd or Group Slaughter
6
192
0.6%
28
14.6%
145
75.5%
28
14.6 %
19
9.9 %
0
Parallel New Infection
97
12820
42.5%
378
2.9%
11548
90.1%
635
5.0 %
486
3.8 %
408
3.2 %
Parallel - new infection cull area
43
5414
17.9%
149
2.8%
4428
81.8%
257
4.7 %
192
3.5 %
645
11.9 %
Parallel - Other
10
1042
3.5%
31
3.0%
979
94.0%
44
4.2 %
32
3.1 %
0
Parallel Persistent Infection
51
6404
21.2%
299
4.7%
5445
85.0%
428
6.7 %
324
5.1 %
336
5.2 %
Parallel - persistent infection cull area
14
3627
12.0%
116
3.2%
3370
92.9%
204
5.6 %
137
3.8 %
4
0.1 %
Parallel - Rapid Testing Two-times IRs
37
135
0.4%
28
20.7%
100
74.1%
11
8.1 %
7
5.2 %
0
Serial - Anomalous Reaction
1
9
0.0%
6
66.7%
2
22.2%
1
11.1 %
1
11.1 %
0
Total
272
30186
100.0%
1080
3.6%
26482
87.7%
1628
5.4 %
1214
4.0 %
1410
4.7 %
Table 4a. Summaryof IFN Gamma results by submission reason (November 2019)
England - HRA
Extended Flexible IFN-GAMMA test
3
299
1.0%
23
7.7%
272
91.0%
6
2.0 %
4
1.3 %
0
Parallel New Infection
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - new infection cull area
40
4003
13.3%
127
3.2%
3096
77.3%
184
4.6 %
135
3.4 %
645
16.1 %
Parallel - Other
2
560
1.9%
4
0.7%
548
97.9%
15
2.7 %
8
1.4 %
0
Parallel Persistent Infection
20
2813
9.3%
139
4.9%
2412
85.7%
164
5.8 %
130
4.6 %
132
4.7 %
Parallel - persistent infection cull area
14
3627
12.0%
116
3.2%
3370
92.9%
204
5.6 %
137
3.8 %
4
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
6
66.7%
2
22.2%
1
11.1 %
1
11.1 %
0
England - HRA
81
11313
37.5%
415
3.7%
9702
85.8%
574
5.1 %
415
3.7 %
781
6.9 %
England - Edge
Parallel New Infection
77
9993
33.1%
324
3.2%
9007
90.1%
517
5.2 %
397
4.0 %
265
2.7 %
Parallel - new infection cull area
3
1411
4.7%
22
1.6%
1332
94.4%
73
5.2 %
57
4.0 %
0
England - Edge
80
11404
37.8%
346
3.0%
10339
90.7%
590
5.2 %
454
4.0 %
265
2.3 %
England - LRA
Parallel New Infection
3
353
1.2%
10
2.8%
336
95.2%
13
3.7 %
7
2.0 %
0
England - LRA
3
353
1.2%
10
2.8%
336
95.2%
13
3.7 %
7
2.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
8
210
0.7%
21
10.0%
177
84.3%
14
6.7 %
12
5.7 %
0
Parallel - Herd or Group Slaughter
6
192
0.6%
28
14.6%
145
75.5%
28
14.6 %
19
9.9 %
0
Parallel New Infection
1
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Parallel - Other
5
244
0.8%
22
9.0%
208
85.2%
19
7.8 %
14
5.7 %
0
Parallel Persistent Infection
24
3251
10.8%
136
4.2%
2737
84.2%
235
7.2 %
174
5.4 %
204
6.3 %
Parallel - Rapid Testing Two-times IRs
32
126
0.4%
26
20.6%
93
73.8%
11
8.7 %
7
5.6 %
0
Wales - High TB
76
4027
13.3%
233
5.8%
3364
83.5%
307
7.6 %
226
5.6 %
204
5.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
34
0.1%
1
2.9%
16
47.1%
0
0
17
50.0 %
Parallel New Infection
13
2452
8.1%
44
1.8%
2185
89.1%
103
4.2 %
80
3.3 %
143
5.8 %
Parallel - Other
3
238
0.8%
5
2.1%
223
93.7%
10
4.2 %
10
4.2 %
0
Parallel Persistent Infection
7
340
1.1%
24
7.1%
296
87.1%
29
8.5 %
20
5.9 %
0
Parallel - Rapid Testing Two-times IRs
5
9
0.0%
2
22.2%
7
77.8%
0
0
0
Wales - Intermediate TB
30
3073
10.2%
76
2.5%
2727
88.7%
142
4.6 %
110
3.6 %
160
5.2 %
Wales - Low TB
Parallel New Infection
1
11
0.0%
0
0.0 %
9
81.8%
2
18.2 %
2
18.2 %
0
Wales - Low TB
1
11
0.0%
0
0.0 %
9
81.8%
2
18.2 %
2
18.2 %
0
Scotland - Scotland
Parallel New Infection
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Scotland - Scotland
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
199
6363
2.1%
430
6.8%
5584
87.8%
317
5.0 %
231
3.6 %
118
1.9 %
Parallel - Herd or Group Slaughter
150
7534
2.5%
462
6.1%
6691
88.8%
503
6.7 %
371
4.9 %
11
0.1 %
Parallel New Infection
1187 139614
45.5%
4419
3.2%
125688
90.0%
9928
7.1 %
7808
5.6 %
1721
1.2 %
Parallel - new infection cull area
560
66128
21.6%
2917
4.4%
58394
88.3%
4687
7.1 %
3613
5.5 %
1204
1.8 %
Parallel - Other
99
5216
1.7%
309
5.9%
4438
85.1%
529
10.1 %
427
8.2 %
42
0.8 %
Parallel Persistent Infection
521
59154
19.3%
2594
4.4%
52718
89.1%
3719
6.3 %
2852
4.8 %
1002
1.7 %
Parallel - persistent infection cull area
106
21342
7.0%
1023
4.8%
19239
90.1%
1408
6.6 %
1069
5.0 %
11
0.1 %
Parallel - Rapid Testing Two-times IRs
409
1385
0.5%
250
18.1%
1031
74.4%
89
6.4 %
65
4.7 %
39
2.8 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
12410
4.0%
273813
89.3%
21182
6.9 %
xx-xxxx-xxxx
5.4 %
4148
1.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
27
1933
0.6%
102
5.3%
1720
89.0%
103
5.3 %
66
3.4 %
45
2.3 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
27
2104
0.7%
139
6.6%
1757
83.5%
264
12.5 %
208
9.9 %
0
Parallel - new infection cull area
555
64419
21.0%
2874
4.5%
56804
88.2%
4593
7.1 %
3537
5.5 %
1204
1.9 %
Parallel - Other
28
2227
0.7%
92
4.1%
1831
82.2%
316
14.2 %
262
11.8 %
42
1.9 %
Parallel Persistent Infection
155
24911
8.1%
1250
5.0%
21761
87.4%
1614
6.5 %
1228
4.9 %
673
2.7 %
Parallel - persistent infection cull area
105
20990
6.8%
1016
4.8%
18899
90.0%
1400
6.7 %
1064
5.1 %
11
0.1 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
England - Edge
Parallel New Infection
888 113105
36.9%
3537
3.1%
101990
90.2%
7839
6.9 %
6138
5.4 %
1458
1.3 %
Parallel - new infection cull area
5
1709
0.6%
43
2.5%
1590
93.0%
94
5.5 %
76
4.4 %
0
Parallel Persistent Infection
18
2026
0.7%
54
2.7%
1879
92.7%
114
5.6 %
90
4.4 %
3
0.1 %
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
82
9717
3.2%
274
2.8%
8610
88.6%
966
9.9 %
795
8.2 %
38
0.4 %
Parallel Persistent Infection
7
893
0.3%
7
0.8%
704
78.8%
108
12.1 %
89
10.0 %
93
10.4 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
England
1911 244179
79.6%
9403
3.9%
217668
89.1%
17421
7.1 %
13560
5.6 %
3567
1.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
119
3139
1.0%
226
7.2%
2797
89.1%
148
4.7 %
113
3.6 %
3
0.1 %
Parallel - Herd or Group Slaughter
138
7376
2.4%
437
5.9%
6574
89.1%
485
6.6 %
355
4.8 %
11
0.1 %
Parallel New Infection
38
1423
0.5%
81
5.7%
1284
90.2%
67
4.7 %
57
4.0 %
1
0.1 %
Parallel - Other
45
2130
0.7%
160
7.5%
1854
87.0%
155
7.3 %
116
5.4 %
0
Parallel Persistent Infection
309
27260
8.9%
1141
4.2%
24597
90.2%
1666
6.1 %
1291
4.7 %
233
0.9 %
Parallel - Rapid Testing Two-times IRs
355
1107
0.4%
223
20.1%
788
71.2%
81
7.3 %
57
5.1 %
39
3.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
49
1152
0.4%
97
8.4%
937
81.3%
59
5.1 %
48
4.2 %
70
6.1 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
101
9986
3.3%
277
2.8%
9056
90.7%
591
5.9 %
455
4.6 %
198
2.0 %
Parallel - Other
23
824
0.3%
56
6.8%
721
87.5%
54
6.6 %
47
5.7 %
0
Parallel Persistent Infection
29
3297
1.1%
133
4.0%
3039
92.2%
193
5.9 %
134
4.1 %
0
Parallel - Rapid Testing Two-times IRs
50
273
0.1%
27
9.9%
238
87.2%
8
2.9 %
8
2.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
31
1276
0.4%
30
2.4%
1171
91.8%
89
7.0 %
63
4.9 %
16
1.3 %
Parallel - Other
3
35
0.0%
1
2.9%
32
91.4%
4
11.4 %
2
5.7 %
0
Parallel Persistent Infection
2
429
0.1%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
4
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Wales
1304
59902
19.5%
2917
4.9%
53656
89.6%
3634
6.1 %
2774
4.6 %
571
1.0 %
Scotland - Scotland
Parallel New Infection
20
2003
0.7%
81
4.0%
1820
90.9%
112
5.6 %
92
4.6 %
10
0.5 %
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Parallel - persistent infection cull area
1
352
0.1%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland
22
2693
0.9%
90
3.3%
2489
92.4%
127
4.7 %
104
3.9 %
10
0.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 December
228
21554
147
1241
6
8
0
0
0
0
92
2
0
7
1
1037
0
82
0
5
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
1
0
4
0
773
0
73
0
0
July
316
32549
201
1219
1
13
0
0
0
0
108
3
0
12
0
1020
0
58
0
2
August
262
21887
126
724
1
8
0
0
0
0
84
0
0
6
1
570
0
52
0
0
September
245
25317
134
693
1
7
0
0
0
0
94
0
0
3
1
542
9
36
0
0
October
298
26690
154
743
3
0
0
0
0
58
0
5
1
0
601
74
0
0
0
November
272
30186
169
1080
199
434
0
0
0
0
31
0
2
0
0
291
9
0
0
0
Totals (last
12 months)
3465
328328
2022
13651
221
508
0
0
0
0
1134
17
7
59
3
10852
98
607
2
14
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2033
259453
1307
10227
371
0
0
0
0
969
10
2
39
1
8519
20
71
0
1
Scotland
22
2693
11
90
0
0
0
0
0
12
0
0
2
0
70
0
5
0
0
Wales
1410
66182
704
3334
137
0
0
0
0
153
7
5
18
2
2263
78
531
2
13
Totals
3465
328328
2022
13651
508
0
0
0
0
1134
17
7
59
3
10852
98
607
2
14
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
35
40
Worcs
Wiltshire
W Glamorgan
Warks
Suffolk
Somerset excl N
Shropshire
Powys
Pembs
Oxon
Northants
Norfolk
NE Wales
Lincs
Leics & Rut
Kirkcudbright
Heref
Hampshire
Gwynedd
Gwent
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during November 2019 (% submission per
County)
County
%Resampled %Retested
Avon
11.11 %
11.11 %
Bucks
3.10 %
3.49 %
Carms
4.48 %
6.99 %
Ceredigion
3.48 %
4.75 %
Cheshire
3.18 %
4.41 %
Corn & Scilly
4.53 %
5.36 %
Derbyshire
4.79 %
5.90 %
Devon
4.55 %
6.34 %
Dorset
2.52 %
3.31 %
Gloucs
6.51 %
7.69 %
Gwent
3.87 %
4.42 %
Gwynedd
18.18 %
18.18 %
Hampshire
Heref
3.19 %
5.32 %
Kirkcudbright
Leics & Rut
6.92 %
8.36 %
Lincs
0.72 %
2.17 %
NE Wales
3.53 %
4.20 %
Norfolk
4.88 %
4.88 %
Northants
2.07 %
3.73 %
Oxon
4.22 %
5.20 %
Pembs
6.28 %
8.43 %
Powys
3.34 %
4.91 %
Shropshire
5.84 %
7.61 %
Somerset excl
1.13 %
1.39 %
Suffolk
8.33 %
13.89 %
Warks
4.61 %
6.35 %
W Glamorgan
12.20 %
17.07 %
Wiltshire
2.87 %
4.92 %
Worcs
1.03 %
2.06 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22854
771
3.4%
23070
771
3.3%
Wales
6848
309
4.5%
7111
309
4.3%
Scotland
5
0
0.0 %
5
0.0 %
GB
29707
1080
3.6%
30186
1080
3.6%
Table 6a (i): Number of animals and submissions tested in November 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
215085
9403
4.4%
244179
9403
3.9%
Wales
54800
2917
5.3%
59902
2917
4.9%
Scotland
2015
90
4.5%
2693
90
3.3%
GB
271893
12410
4.6%
306774
12410
4.0%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
17996
1078
6.0%
18356
1078
5.9%
Wales
6081
502
8.3%
6214
502
8.1%
GB
24076
1580
6.6%
24570
1580
6.4%
Table 6a (ii): Number of animals and submissions tested in November 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
154045
8796
5.7%
178364
8796
4.9%
Wales
44614
2443
5.5%
51327
2452
4.8%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
200719
11420
5.7%
231932
11429
4.9%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SP
1
1
0
0
Somerset excl North
PRV-SP
2
4
0
0
Total
4
7
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples November 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
2
7
0
0
PRV-SP
3
3
0
0
Dorset
PRV-SP
2
4
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SP
2
2
0
0
Somerset excl North
PRV-SE
6
247
16
6.48 %
PRV-SP
7
12
0
0
Staffordshire
PRV-SE
1
64
3
4.69 %
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
27
353
19
5.38 %
Isle of Man
SER-IOM
27
370
2
0.54 %
Isle of Man
SER-IOM
1
2
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
12
1
8.3%
Cornwall and Isles of
1
237
30
12.7%
North East Wales
3
78
3
3.8%
Pembrokeshire
2
149
30
20.1%
Powys
1
13
3
23.1%
TC0077
Sum:
489
67
13.7 %
Table 8a Number of Cattle Antibody Tests November 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
16
575
53
9.2%
Ceredigion
3
372
17
4.6%
Cornwall and Isles of
1
237
30
12.7%
North East Wales
30
974
119
12.2%
Pembrokeshire
21
767
65
8.5%
Powys
9
380
27
7.1%
South Wales
5
196
16
8.2%
Wiltshire
1
248
9
3.6%
TC0077
Sum:
3,749
336
9.0 %
Table 8b Number of Cattle Antibody Tests 2019