Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - January 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 173
Report Period 1st - 31st January 2021
Issued 19 February 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
Parallel blood testing of skin test-negative cattle
in breakdowns herds occurring within 6 months
of lifting restrictions after a previous breakdown
NO (APHA discretion -
Wales only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Test Criteria

Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
January
2021
%
Total 2021
%
Submissions
375
375
Samples
Total (%)
27940
100.00 %
27940
100.00 %
England
23125
82.77 %
23125
82.77 %
Wales
4795
17.16 %
4795
17.16 %
Scotland
20
0.07 %
20
0.07 %
Parallel Tests
PAR-CUL-N
11345
40.60 %
11345
40.60 %
PAR-CUL-P
817
2.92 %
817
2.92 %
PAR-HERD-S
0
0.00 %
PAR-NEW-IN
11616
41.57 %
11616
41.57 %
PAR-OTHER
1184
4.24 %
1184
4.24 %
PAR-PERSIS
2644
9.46 %
2644
9.46 %
PAR-RAPID
185
0.66 %
185
0.66 %
Total(% of all samples)
27791
99.47 %
27791
99.47 %
Serial/Extended Tests
SER-FLEXI
149
0.53 %
149
0.53 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
Total(% of all samples)
149
0.53 %
149
0.53 %
Total
27940
100.00 %
27940
100.00 %
Retests
Total (% of all samples)
1958
7.01 %
1958
7.01 %
England
1602
5.73 %
1602
5.73 %
Wales
354
1.27 %
354
1.27 %
Scotland
2
0.01 %
2
0.01 %
Resamples
Total (% of all samples)
xx-xxxx-xxxx
6.05 %
xx-xxxx-xxxx
6.05 %
England
1394
4.99 %
1394
4.99 %
Wales
293
1.05 %
293
1.05 %
Scotland
2
0.01 %
2
0.01 %
Rejects
Total (% of all samples)
77
0.28 %
77
0.28 %
England
42
0.15 %
42
0.15 %
Wales
35
0.13 %
35
0.13 %
Scotland
0
0.00 %
Table 1. Monthly Totals for January 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
11345
40.6 %
PAR-CUL-P
817
2.9 %
PAR-NEW-IN
11616
41.6 %
PAR-OTHER
1184
4.2 %
PAR-PERSIS
2644
9.5 %
PAR-RAPID
185
0.7 %
SER-FLEXI
149
0.5 %
Total
27940
100.00 %
Figure 1. Number of samples submitted in each category during January 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Lanarkshire
Dumfries & G
W Glamorgan
Shropshire
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during January 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Lanarkshire
Dumfries & G
W Glamorgan
Shropshire
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
3
658
2.36 %
121
18.39 %
476
72.34 %
61
9.27 %
52
7.90 %
9
1.37 %
Corn & Scilly
PAR-CUL-N
8
478
1.71 %
22
4.60 %
421
88.08 %
42
8.79 %
34
7.11 %
1
0.21 %
PAR-CUL-P
3
807
2.89 %
33
4.09 %
714
88.48 %
63
7.81 %
58
7.19 %
2
0.25 %
Devon
PAR-CUL-N
34
4,776
17.09 %
348
7.29 %
4191
87.75 %
249
5.21 %
225
4.71 %
12
0.25 %
PAR-CUL-P
1
6
0.02 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Dorset
PAR-CUL-N
9
716
2.56 %
33
4.61 %
648
90.50 %
37
5.17 %
33
4.61 %
2
0.28 %
Gloucs
PAR-CUL-N
7
875
3.13 %
69
7.89 %
746
85.26 %
73
8.34 %
60
6.86 %
0
0.00 %
Heref
PAR-CUL-N
2
113
0.40 %
6
5.31 %
98
86.73 %
9
7.96 %
9
7.96 %
0
0.00 %
PAR-PERSIS
1
100
0.36 %
6
6.00 %
82
82.00 %
12
12.00 %
12
12.00 %
0
0.00 %
Shropshire
PAR-CUL-N
8
1,045
3.74 %
42
4.02 %
951
91.00 %
58
5.55 %
51
4.88 %
1
0.10 %
Somerset excl N
PAR-CUL-N
8
813
2.91 %
43
5.29 %
744
91.51 %
33
4.06 %
26
3.20 %
0
0.00 %
PAR-OTHER
1
418
1.50 %
21
5.02 %
348
83.25 %
27
6.46 %
49
11.72 %
0
0.00 %
Staffs
PAR-CUL-N
9
1,196
4.28 %
79
6.61 %
1016
84.95 %
111
9.28 %
101
8.44 %
0
0.00 %
PAR-CUL-P
1
4
0.01 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Wiltshire
PAR-CUL-N
9
1,209
4.33 %
104
8.60 %
1035
85.61 %
90
7.44 %
70
5.79 %
0
0.00 %
PAR-NEW-IN
1
182
0.65 %
25
13.74 %
148
81.32 %
11
6.04 %
9
4.95 %
0
0.00 %
PAR-PERSIS
1
23
0.08 %
3
13.04 %
14
60.87 %
7
30.43 %
6
26.09 %
0
0.00 %
Worcs
PAR-CUL-N
1
124
0.44 %
11
8.87 %
109
87.90 %
3
2.42 %
2
1.61 %
2
1.61 %
England - Edge
Berks
PAR-NEW-IN
2
109
0.39 %
14
12.84 %
80
73.39 %
16
14.68 %
15
13.76 %
0
0.00 %
Bucks
PAR-NEW-IN
1
78
0.28 %
4
5.13 %
72
92.31 %
2
2.56 %
2
2.56 %
0
0.00 %
Cheshire
PAR-NEW-IN
7
848
3.04 %
45
5.31 %
766
90.33 %
50
5.90 %
37
4.36 %
0
0.00 %
Derbyshire
PAR-NEW-IN
13
1,042
3.73 %
51
4.89 %
897
86.08 %
119
11.42 %
94
9.02 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
18
0.06 %
3
16.67 %
11
61.11 %
5
27.78 %
4
22.22 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
1,361
4.87 %
43
3.16 %
1201
88.24 %
126
9.26 %
115
8.45 %
2
0.15 %
PAR-PERSIS
1
76
0.27 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
763
2.73 %
21
2.75 %
722
94.63 %
30
3.93 %
20
2.62 %
0
0.00 %
Table 2a. Summary by County for January 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
9
656
2.35 %
26
3.96 %
592
90.24 %
42
6.40 %
38
5.79 %
0
0.00 %
Notts
PAR-NEW-IN
1
12
0.04 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Oxon
PAR-NEW-IN
16
1,555
5.57 %
46
2.96 %
1417
91.13 %
100
6.43 %
88
5.66 %
4
0.26 %
Warks
PAR-NEW-IN
10
680
2.43 %
30
4.41 %
588
86.47 %
65
9.56 %
55
8.09 %
7
1.03 %
England - LRA
Cleveland
PAR-NEW-IN
1
186
0.67 %
5
2.69 %
173
93.01 %
10
5.38 %
8
4.30 %
0
0.00 %
Cumbria
PAR-NEW-IN
8
1,340
4.80 %
42
3.13 %
1246
92.99 %
70
5.22 %
52
3.88 %
0
0.00 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.34 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
41
0.15 %
2
4.88 %
37
90.24 %
3
7.32 %
2
4.88 %
0
0.00 %
Herts
PAR-NEW-IN
1
91
0.33 %
9
9.89 %
81
89.01 %
1
1.10 %
1
1.10 %
0
0.00 %
Lancashire
PAR-NEW-IN
2
42
0.15 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Lincs
PAR-NEW-IN
4
344
1.23 %
17
4.94 %
308
89.53 %
24
6.98 %
19
5.52 %
0
0.00 %
N Yorks
PAR-NEW-IN
7
246
0.88 %
26
10.57 %
188
76.42 %
36
14.63 %
32
13.01 %
0
0.00 %
England
206
23125
82.77 %
1354
5.86 %
20335
87.94 %
1602
6.93 %
1394
6.03 %
42
0.18 %
Wales - High TB
Carms
PAR-NEW-IN
1
6
0.02 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
5
151
0.54 %
15
9.93 %
118
78.15 %
17
11.26 %
16
10.60 %
2
1.32 %
PAR-PERSIS
3
11
0.04 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
11
15
0.05 %
2
13.33 %
13
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
5
476
1.70 %
34
7.14 %
409
85.92 %
29
6.09 %
20
4.20 %
13
2.73 %
PAR-RAPID
8
21
0.08 %
5
23.81 %
15
71.43 %
2
9.52 %
1
4.76 %
0
0.00 %
Gwent
PAR-PERSIS
7
98
0.35 %
7
7.14 %
85
86.73 %
5
5.10 %
5
5.10 %
1
1.02 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
11
564
2.02 %
28
4.96 %
511
90.60 %
30
5.32 %
23
4.08 %
2
0.35 %
PAR-PERSIS
22
185
0.66 %
40
21.62 %
137
74.05 %
10
5.41 %
8
4.32 %
0
0.00 %
PAR-RAPID
25
126
0.45 %
32
25.40 %
91
72.22 %
4
3.17 %
3
2.38 %
0
0.00 %
Powys
PAR-NEW-IN
1
239
0.86 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
3
47
0.17 %
4
8.51 %
43
91.49 %
2
4.26 %
0
0.00 %
0
0.00 %
PAR-PERSIS
18
732
2.62 %
19
2.60 %
647
88.39 %
63
8.61 %
59
8.06 %
7
0.96 %
PAR-RAPID
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-PERSIS
2
136
0.49 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-PERSIS
2
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
SER-FLEXI
3
148
0.53 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
NE Wales
PAR-NEW-IN
9
363
1.30 %
12
3.31 %
329
90.63 %
26
7.16 %
22
6.06 %
0
0.00 %
PAR-OTHER
2
3
0.01 %
0
0.00 %
1
33.33 %
2
66.67 %
2
66.67 %
0
0.00 %
PAR-PERSIS
2
146
0.52 %
1
0.68 %
143
97.95 %
4
2.74 %
2
1.37 %
0
0.00 %
PAR-RAPID
1
19
0.07 %
3
15.79 %
15
78.95 %
1
5.26 %
1
5.26 %
0
0.00 %
Powys
PAR-NEW-IN
3
190
0.68 %
4
2.11 %
171
90.00 %
17
8.95 %
14
7.37 %
1
0.53 %
Shropshire
PAR-NEW-IN
1
110
0.39 %
4
3.64 %
89
80.91 %
14
12.73 %
13
11.82 %
4
3.64 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
225
0.81 %
10
4.44 %
192
85.33 %
32
14.22 %
23
10.22 %
0
0.00 %
NE Wales
PAR-NEW-IN
10
775
2.77 %
59
7.61 %
659
85.03 %
68
8.77 %
54
6.97 %
3
0.39 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
167
4795
17.16 %
298
6.21 %
4169
86.94 %
354
7.38 %
293
6.11 %
35
0.73 %
Scotland - Scotland
Dumfries & G
PAR-NEW-IN
1
8
0.03 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.04 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
2
20
0.07 %
1
5.00 %
17
85.00 %
2
10.00 %
2
10.00 %
0
0.00 %
Grand Total
Sum:
375
27940
1653
24521
1958
xx-xxxx-xxxx
77
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
3
658
2.36 %
121
18.39 %
476
72.34 %
61
9.27 %
52
7.90 %
9
1.37 %
Corn & Scilly
PAR-CUL-N
8
478
1.71 %
22
4.60 %
421
88.08 %
42
8.79 %
34
7.11 %
1
0.21 %
PAR-CUL-P
3
807
2.89 %
33
4.09 %
714
88.48 %
63
7.81 %
58
7.19 %
2
0.25 %
Devon
PAR-CUL-N
34
4,776
17.09 %
348
7.29 %
4191
87.75 %
249
5.21 %
225
4.71 %
12
0.25 %
PAR-CUL-P
1
6
0.02 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Dorset
PAR-CUL-N
9
716
2.56 %
33
4.61 %
648
90.50 %
37
5.17 %
33
4.61 %
2
0.28 %
Gloucs
PAR-CUL-N
7
875
3.13 %
69
7.89 %
746
85.26 %
73
8.34 %
60
6.86 %
0
0.00 %
Heref
PAR-CUL-N
2
113
0.40 %
6
5.31 %
98
86.73 %
9
7.96 %
9
7.96 %
0
0.00 %
PAR-PERSIS
1
100
0.36 %
6
6.00 %
82
82.00 %
12
12.00 %
12
12.00 %
0
0.00 %
Shropshire
PAR-CUL-N
8
1,045
3.74 %
42
4.02 %
951
91.00 %
58
5.55 %
51
4.88 %
1
0.10 %
Somerset excl N
PAR-CUL-N
8
813
2.91 %
43
5.29 %
744
91.51 %
33
4.06 %
26
3.20 %
0
0.00 %
PAR-OTHER
1
418
1.50 %
21
5.02 %
348
83.25 %
27
6.46 %
49
11.72 %
0
0.00 %
Staffs
PAR-CUL-N
9
1,196
4.28 %
79
6.61 %
1016
84.95 %
111
9.28 %
101
8.44 %
0
0.00 %
PAR-CUL-P
1
4
0.01 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Wiltshire
PAR-CUL-N
9
1,209
4.33 %
104
8.60 %
1035
85.61 %
90
7.44 %
70
5.79 %
0
0.00 %
PAR-NEW-IN
1
182
0.65 %
25
13.74 %
148
81.32 %
11
6.04 %
9
4.95 %
0
0.00 %
PAR-PERSIS
1
23
0.08 %
3
13.04 %
14
60.87 %
7
30.43 %
6
26.09 %
0
0.00 %
Worcs
PAR-CUL-N
1
124
0.44 %
11
8.87 %
109
87.90 %
3
2.42 %
2
1.61 %
2
1.61 %
England - Edge
Berks
PAR-NEW-IN
2
109
0.39 %
14
12.84 %
80
73.39 %
16
14.68 %
15
13.76 %
0
0.00 %
Bucks
PAR-NEW-IN
1
78
0.28 %
4
5.13 %
72
92.31 %
2
2.56 %
2
2.56 %
0
0.00 %
Cheshire
PAR-NEW-IN
7
848
3.04 %
45
5.31 %
766
90.33 %
50
5.90 %
37
4.36 %
0
0.00 %
Derbyshire
PAR-NEW-IN
13
1,042
3.73 %
51
4.89 %
897
86.08 %
119
11.42 %
94
9.02 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
18
0.06 %
3
16.67 %
11
61.11 %
5
27.78 %
4
22.22 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
1,361
4.87 %
43
3.16 %
1201
88.24 %
126
9.26 %
115
8.45 %
2
0.15 %
PAR-PERSIS
1
76
0.27 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
763
2.73 %
21
2.75 %
722
94.63 %
30
3.93 %
20
2.62 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
9
656
2.35 %
26
3.96 %
592
90.24 %
42
6.40 %
38
5.79 %
0
0.00 %
Notts
PAR-NEW-IN
1
12
0.04 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Oxon
PAR-NEW-IN
16
1,555
5.57 %
46
2.96 %
1417
91.13 %
100
6.43 %
88
5.66 %
4
0.26 %
Warks
PAR-NEW-IN
10
680
2.43 %
30
4.41 %
588
86.47 %
65
9.56 %
55
8.09 %
7
1.03 %
England - LRA
Cleveland
PAR-NEW-IN
1
186
0.67 %
5
2.69 %
173
93.01 %
10
5.38 %
8
4.30 %
0
0.00 %
Cumbria
PAR-NEW-IN
8
1,340
4.80 %
42
3.13 %
1246
92.99 %
70
5.22 %
52
3.88 %
0
0.00 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.34 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
41
0.15 %
2
4.88 %
37
90.24 %
3
7.32 %
2
4.88 %
0
0.00 %
Herts
PAR-NEW-IN
1
91
0.33 %
9
9.89 %
81
89.01 %
1
1.10 %
1
1.10 %
0
0.00 %
Lancashire
PAR-NEW-IN
2
42
0.15 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Lincs
PAR-NEW-IN
4
344
1.23 %
17
4.94 %
308
89.53 %
24
6.98 %
19
5.52 %
0
0.00 %
N Yorks
PAR-NEW-IN
7
246
0.88 %
26
10.57 %
188
76.42 %
36
14.63 %
32
13.01 %
0
0.00 %
England
206
23125
82.77 %
1354
5.86 %
20335
87.94 %
1602
6.93 %
1394
6.03 %
42
0.18 %
Wales - High TB
Carms
PAR-NEW-IN
1
6
0.02 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
5
151
0.54 %
15
9.93 %
118
78.15 %
17
11.26 %
16
10.60 %
2
1.32 %
PAR-PERSIS
3
11
0.04 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
11
15
0.05 %
2
13.33 %
13
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
5
476
1.70 %
34
7.14 %
409
85.92 %
29
6.09 %
20
4.20 %
13
2.73 %
PAR-RAPID
8
21
0.08 %
5
23.81 %
15
71.43 %
2
9.52 %
1
4.76 %
0
0.00 %
Gwent
PAR-PERSIS
7
98
0.35 %
7
7.14 %
85
86.73 %
5
5.10 %
5
5.10 %
1
1.02 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
11
564
2.02 %
28
4.96 %
511
90.60 %
30
5.32 %
23
4.08 %
2
0.35 %
PAR-PERSIS
22
185
0.66 %
40
21.62 %
137
74.05 %
10
5.41 %
8
4.32 %
0
0.00 %
PAR-RAPID
25
126
0.45 %
32
25.40 %
91
72.22 %
4
3.17 %
3
2.38 %
0
0.00 %
Powys
PAR-NEW-IN
1
239
0.86 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
3
47
0.17 %
4
8.51 %
43
91.49 %
2
4.26 %
0
0.00 %
0
0.00 %
PAR-PERSIS
18
732
2.62 %
19
2.60 %
647
88.39 %
63
8.61 %
59
8.06 %
7
0.96 %
PAR-RAPID
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-PERSIS
2
136
0.49 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-PERSIS
2
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
SER-FLEXI
3
148
0.53 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
NE Wales
PAR-NEW-IN
9
363
1.30 %
12
3.31 %
329
90.63 %
26
7.16 %
22
6.06 %
0
0.00 %
PAR-OTHER
2
3
0.01 %
0
0.00 %
1
33.33 %
2
66.67 %
2
66.67 %
0
0.00 %
PAR-PERSIS
2
146
0.52 %
1
0.68 %
143
97.95 %
4
2.74 %
2
1.37 %
0
0.00 %
PAR-RAPID
1
19
0.07 %
3
15.79 %
15
78.95 %
1
5.26 %
1
5.26 %
0
0.00 %
Powys
PAR-NEW-IN
3
190
0.68 %
4
2.11 %
171
90.00 %
17
8.95 %
14
7.37 %
1
0.53 %
Shropshire
PAR-NEW-IN
1
110
0.39 %
4
3.64 %
89
80.91 %
14
12.73 %
13
11.82 %
4
3.64 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
225
0.81 %
10
4.44 %
192
85.33 %
32
14.22 %
23
10.22 %
0
0.00 %
NE Wales
PAR-NEW-IN
10
775
2.77 %
59
7.61 %
659
85.03 %
68
8.77 %
54
6.97 %
3
0.39 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
167
4795
17.16 %
298
6.21 %
4169
86.94 %
354
7.38 %
293
6.11 %
35
0.73 %
Scotland - Scotland
Dumfries & G
PAR-NEW-IN
1
8
0.03 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.04 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
2
20
0.07 %
1
5.00 %
17
85.00 %
2
10.00 %
2
10.00 %
0
0.00 %
Grand Total
Sum:
375
27940
1653
24521
1958
xx-xxxx-xxxx
77
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol January 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
206
23125
100.0%
1354
5.9%
20335
87.9%
1602
6.9%
1394
6.0%
42
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
206
23125
100.0%
1354
5.9%
20335
87.9%
1602
6.9%
1394
6.0%
42
0.2%
Scotland
Parallel
2
20
100.0%
1
5.0%
17
85.0%
2
10.0%
2
10.0%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
20
100.0%
1
5.0%
17
85.0%
2
10.0%
2
10.0%
0
0.0 %
Wales
Parallel
163
4646
96.9%
292
6.3%
4029
86.7%
351
7.6%
291
6.3%
34
0.7%
Serial/Extended
4
149
3.1%
6
4.0%
140
94.0%
3
2.0%
2
1.3%
1
0.7%
Total
167
4795
100.0%
298
6.2%
4169
86.9%
354
7.4%
293
6.1%
35
0.7%
GB
Parallel
371
27791
99.5%
xx-xxxx-xxxx
5.9%
24381
87.7%
1955
7.0%
xx-xxxx-xxxx
6.1%
76
0.3%
Serial/Extended
4
149
0.5%
6
4.0%
140
94.0%
3
2.0%
2
1.3%
1
0.7%
Total
375
27940
100.0%
1653
5.9%
24521
87.8%
1958
7.0%
xx-xxxx-xxxx
6.0%
77
0.3%
206.00
167.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
206
23125
100.0%
1354
5.9%
20335
87.9%
1602
6.9%
1394
6.0%
42
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
206
23125
100.0%
1354
5.9%
20335
87.9%
1602
6.9%
1394
6.0%
42
0.2%
Scotland
Parallel
2
20
100.0%
1
5.0%
17
85.0%
2
10.0%
2
10.0%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
20
100.0%
1
5.0%
17
85.0%
2
10.0%
2
10.0%
0
0.0 %
Wales
Parallel
163
4646
96.9%
292
6.3%
4029
86.7%
351
7.6%
291
6.3%
34
0.7%
Serial/Extended
4
149
3.1%
6
4.0%
140
94.0%
3
2.0%
2
1.3%
1
0.7%
Total
167
4795
100.0%
298
6.2%
4169
86.9%
354
7.4%
293
6.1%
35
0.7%
GB
Parallel
371
27791
99.5%
xx-xxxx-xxxx
5.9%
24381
87.7%
1955
7.0%
xx-xxxx-xxxx
6.1%
76
0.3%
Serial/Extended
4
149
0.5%
6
4.0%
140
94.0%
3
2.0%
2
1.3%
1
0.7%
Total
375
27940
100.0%
1653
5.9%
24521
87.8%
1958
7.0%
xx-xxxx-xxxx
6.0%
77
0.3%
206.00
167.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
4
149
0.5%
6
4.0%
140
94.0%
3
2.0 %
2
1.3 %
1
0.7 %
Parallel New Infection
132
11616
41.6%
506
4.4%
10340
89.0%
898
7.7 %
748
6.4 %
22
0.2 %
Parallel - new infection cull area
95
11345
40.6%
757
6.7%
9959
87.8%
705
6.2 %
611
5.4 %
18
0.2 %
Parallel - Other
23
1184
4.2%
68
5.7%
1022
86.3%
78
6.6 %
90
7.6 %
4
0.3 %
Parallel Persistent Infection
67
2644
9.5%
241
9.1%
2200
83.2%
202
7.6 %
173
6.5 %
30
1.1 %
Parallel - persistent infection cull area
5
817
2.9%
33
4.0%
722
88.4%
65
8.0 %
60
7.3 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
49
185
0.7%
42
22.7%
138
74.6%
7
3.8 %
5
2.7 %
0
Total
375
27940
100.0%
1653
5.9%
24521
87.8%
1958
7.0 %
xx-xxxx-xxxx
6.0 %
77
0.3 %
Table 4a. Summaryof IFN Gamma results by submission reason (January 2021)
England - HRA
Parallel New Infection
1
182
0.7%
25
13.7%
148
81.3%
11
6.0 %
9
4.9 %
0
Parallel - new infection cull area
95
11345
40.6%
757
6.7%
9959
87.8%
705
6.2 %
611
5.4 %
18
0.2 %
Parallel - Other
1
418
1.5%
21
5.0%
348
83.3%
27
6.5 %
49
11.7 %
0
Parallel Persistent Infection
5
781
2.8%
130
16.6%
572
73.2%
80
10.2 %
70
9.0 %
9
1.2 %
Parallel - persistent infection cull area
5
817
2.9%
33
4.0%
722
88.4%
65
8.0 %
60
7.3 %
2
0.2 %
England - HRA
107
13543
48.5%
966
7.1%
11749
86.8%
888
6.6 %
799
5.9 %
29
0.2 %
England - Edge
Parallel New Infection
72
7122
25.5%
283
4.0%
6355
89.2%
558
7.8 %
471
6.6 %
13
0.2 %
Parallel Persistent Infection
1
76
0.3%
3
3.9%
69
90.8%
6
7.9 %
4
5.3 %
0
England - Edge
73
7198
25.8%
286
4.0%
6424
89.2%
564
7.8 %
475
6.6 %
13
0.2 %
England - LRA
Parallel New Infection
26
2384
8.5%
102
4.3%
2162
90.7%
150
6.3 %
120
5.0 %
0
England - LRA
26
2384
8.5%
102
4.3%
2162
90.7%
150
6.3 %
120
5.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
2
245
0.9%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
19
762
2.7%
47
6.2%
672
88.2%
49
6.4 %
39
5.1 %
4
0.5 %
Parallel Persistent Infection
57
1638
5.9%
105
6.4%
1415
86.4%
112
6.8 %
97
5.9 %
21
1.3 %
Parallel - Rapid Testing Two-times IRs
47
165
0.6%
39
23.6%
122
73.9%
6
3.6 %
4
2.4 %
0
Wales - High TB
126
2811
10.1%
197
7.0%
2428
86.4%
187
6.7 %
160
5.7 %
26
0.9 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
148
0.5%
6
4.1%
139
93.9%
3
2.0 %
2
1.4 %
1
0.7 %
Parallel New Infection
13
663
2.4%
20
3.0%
589
88.8%
57
8.6 %
49
7.4 %
5
0.8 %
Parallel - Other
2
3
0.0%
0
0.0 %
1
33.3%
2
66.7 %
2
66.7 %
0
Parallel Persistent Infection
4
149
0.5%
3
2.0%
144
96.6%
4
2.7 %
2
1.3 %
0
Parallel - Rapid Testing Two-times IRs
2
20
0.1%
3
15.0%
16
80.0%
1
5.0 %
1
5.0 %
0
Wales - Intermediate TB
24
983
3.5%
32
3.3%
889
90.4%
67
6.8 %
56
5.7 %
6
0.6 %
Wales - Low TB
Parallel New Infection
16
1000
3.6%
69
6.9%
851
85.1%
100
10.0 %
77
7.7 %
3
0.3 %
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
17
1001
3.6%
69
6.9%
852
85.1%
100
10.0 %
77
7.7 %
3
0.3 %
Scotland - Scotland
Parallel New Infection
2
20
0.1%
1
5.0%
17
85.0%
2
10.0 %
2
10.0 %
0
Scotland - Scotland
2
20
0.1%
1
5.0%
17
85.0%
2
10.0 %
2
10.0 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
4
149
0.5%
6
4.0%
140
94.0%
3
2.0 %
2
1.3 %
1
0.7 %
Parallel New Infection
132
11616
41.6%
506
4.4%
10340
89.0%
898
7.7 %
748
6.4 %
22
0.2 %
Parallel - new infection cull area
95
11345
40.6%
757
6.7%
9959
87.8%
705
6.2 %
611
5.4 %
18
0.2 %
Parallel - Other
23
1184
4.2%
68
5.7%
1022
86.3%
78
6.6 %
90
7.6 %
4
0.3 %
Parallel Persistent Infection
67
2644
9.5%
241
9.1%
2200
83.2%
202
7.6 %
173
6.5 %
30
1.1 %
Parallel - persistent infection cull area
5
817
2.9%
33
4.0%
722
88.4%
65
8.0 %
60
7.3 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
49
185
0.7%
42
22.7%
138
74.6%
7
3.8 %
5
2.7 %
0
Total
375
27940
100.0%
1653
5.9%
24521
87.8%
1958
7.0 %
xx-xxxx-xxxx
6.0 %
77
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Parallel New Infection
1
182
0.7%
25
13.7%
148
81.3%
11
6.0 %
9
4.9 %
0
Parallel - new infection cull area
95
11345
40.6%
757
6.7%
9959
87.8%
705
6.2 %
611
5.4 %
18
0.2 %
Parallel - Other
1
418
1.5%
21
5.0%
348
83.3%
27
6.5 %
49
11.7 %
0
Parallel Persistent Infection
5
781
2.8%
130
16.6%
572
73.2%
80
10.2 %
70
9.0 %
9
1.2 %
Parallel - persistent infection cull area
5
817
2.9%
33
4.0%
722
88.4%
65
8.0 %
60
7.3 %
2
0.2 %
England - Edge
Parallel New Infection
72
7122
25.5%
283
4.0%
6355
89.2%
558
7.8 %
471
6.6 %
13
0.2 %
Parallel Persistent Infection
1
76
0.3%
3
3.9%
69
90.8%
6
7.9 %
4
5.3 %
0
England - LRA
Parallel New Infection
26
2384
8.5%
102
4.3%
2162
90.7%
150
6.3 %
120
5.0 %
0
England
206
23125
82.8%
1354
5.9%
20335
87.9%
1602
6.9 %
1394
6.0 %
42
0.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
2
245
0.9%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
19
762
2.7%
47
6.2%
672
88.2%
49
6.4 %
39
5.1 %
4
0.5 %
Parallel Persistent Infection
57
1638
5.9%
105
6.4%
1415
86.4%
112
6.8 %
97
5.9 %
21
1.3 %
Parallel - Rapid Testing Two-times IRs
47
165
0.6%
39
23.6%
122
73.9%
6
3.6 %
4
2.4 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
148
0.5%
6
4.1%
139
93.9%
3
2.0 %
2
1.4 %
1
0.7 %
Parallel New Infection
13
663
2.4%
20
3.0%
589
88.8%
57
8.6 %
49
7.4 %
5
0.8 %
Parallel - Other
2
3
0.0%
0
0.0 %
1
33.3%
2
66.7 %
2
66.7 %
0
Parallel Persistent Infection
4
149
0.5%
3
2.0%
144
96.6%
4
2.7 %
2
1.3 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - Rapid Testing Two-times IRs
2
20
0.1%
3
15.0%
16
80.0%
1
5.0 %
1
5.0 %
0
Wales - Low TB
Parallel New Infection
16
1000
3.6%
69
6.9%
851
85.1%
100
10.0 %
77
7.7 %
3
0.3 %
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
167
4795
17.2%
298
6.2%
4169
86.9%
354
7.4 %
293
6.1 %
35
0.7 %
Scotland - Scotland
Parallel New Infection
2
20
0.1%
1
5.0%
17
85.0%
2
10.0 %
2
10.0 %
0
Scotland
2
20
0.1%
1
5.0%
17
85.0%
2
10.0 %
2
10.0 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 February
323
33146
192
1289
1
21
0
0
0
0
187
3
0
6
0
982
0
89
0
0
March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
0
70
0
1
August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
1
65
0
0
October
316
26668
189
1198
9
0
0
0
0
114
4
0
2
2
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
0
1
7
3
1124
3
84
0
1
December
324
24146
186
1228
1
33
0
0
0
0
104
0
8
2
0
1006
72
0
0
1
2021 January
375
27940
233
1653
135
1035
0
0
0
0
19
0
2
0
0
367
42
0
0
0
Totals (last
12 months)
3544
281113
2047
12420
141
1200
0
0
0
0
1219
16
11
47
5
8948
119
652
0
4
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1864
220644
1202
9201
1041
0
0
0
0
1099
7
8
18
4
6798
12
45
0
1
Scotland
55
5029
30
139
0
0
0
0
0
15
0
0
4
1
64
30
24
0
0
Wales
1625
55440
815
3080
159
0
0
0
0
105
9
3
25
0
2086
77
583
0
3
Totals
3544
281113
2047
12420
1200
0
0
0
0
1219
16
11
47
5
8948
119
652
0
4
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25839
1653
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
Worcs
W Glamorgan
Staffs
Shropshire
Pembs
N Yorks
Northants
Lincs
Lancashire
Herts
Hampshire
Gwent
Gloucs
E Riding N Lincs
Dorset
Derbyshire
Corn & Scilly
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during January 2021 (% submission per
County)
County
%Resampled %Retested
Avon
7.90 %
9.27 %
Berks
13.76 %
14.68 %
Bucks
2.56 %
2.56 %
Carms
8.56 %
9.09 %
Ceredigion
3.57 %
5.27 %
Cheshire
4.36 %
5.90 %
Cleveland
4.30 %
5.38 %
Corn & Scilly
7.16 %
8.17 %
Cumbria
3.88 %
5.22 %
Derbyshire
9.02 %
11.42 %
Devon
4.73 %
5.23 %
Dorset
4.61 %
5.17 %
Dumfries & G
12.50 %
12.50 %
E Riding N Linc
3.19 %
3.19 %
E Sussex
22.22 %
27.78 %
Gloucs
6.86 %
8.34 %
Gr Manchester
4.88 %
7.32 %
Gwent
5.05 %
5.05 %
Gwynedd
10.22 %
14.22 %
Hampshire
8.28 %
9.19 %
Heref
9.86 %
9.86 %
Herts
1.10 %
1.10 %
Lanarkshire
8.33 %
8.33 %
Lancashire
7.14 %
7.14 %
Leics & Rut
2.62 %
3.93 %
Lincs
5.52 %
6.98 %
NE Wales
6.20 %
7.73 %
Northants
5.79 %
6.40 %
Notts
25.00 %
25.00 %
N Yorks
13.01 %
14.63 %
Oxon
5.66 %
6.43 %
Pembs
3.89 %
5.03 %
Powys
7.68 %
8.42 %
Shropshire
5.54 %
6.23 %
Somerset excl
6.09 %
4.87 %

Staffs
8.50 %
9.33 %
Warks
8.09 %
9.56 %
W Glamorgan
3.68 %
3.68 %
Wiltshire
6.01 %
7.64 %
Worcs
1.61 %
2.42 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22821
1354
5.9%
23125
1354
5.9%
Wales
4652
298
6.4%
4795
298
6.2%
Scotland
20
1
5.0%
20
1
5.0%
GB
27492
1653
6.0%
27940
1653
5.9%
Table 6a (i): Number of animals and submissions tested in January 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22821
1354
5.9%
23125
1354
5.9%
Wales
4652
298
6.4%
4795
298
6.2%
Scotland
20
1
5.0%
20
1
5.0%
GB
27492
1653
6.0%
27940
1653
5.9%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
23736
1009
4.3%
24247
1009
4.2%
Wales
5668
335
5.9%
5757
335
5.8%
Scotland
1225
23
1.9%
1270
23
1.8%
GB
30629
1367
4.5%
31274
1367
4.4%
Table 6a (ii): Number of animals and submissions tested in January 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
23736
1009
4.3%
24247
1009
4.2%
Wales
5668
335
5.9%
5757
335
5.8%
Scotland
1225
23
1.9%
1270
23
1.8%
GB
30629
1367
4.5%
31274
1367
4.4%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
3
5
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples January 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
3
5
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Devon
1
95
6
6.3%
England
1
95
6
6.3%
Wales
Carmarthenshire
4
16
3
18.8%
Ceredigion
2
16
1
6.3%
North East Wales
2
24
6
25.0%
Pembrokeshire
17
191
29
15.2%
Powys
2
11
1
9.1%
South Wales
4
14
5
35.7%
Wales
31
272
45
16.5%
All GB
32
367
51
13.9 %
Table 8a Number of Cattle Antibody Tests January 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Devon
1
95
6
6.3%
England
1
95
6
6.3%
Wales
Carmarthenshire
4
16
3
18.8%
Ceredigion
2
16
1
6.3%
North East Wales
2
24
6
25.0%
Pembrokeshire
17
191
29
15.2%
Powys
2
11
1
9.1%
South Wales
4
14
5
35.7%
Wales
31
272
45
16.5%
All GB
32
367
51
13.9 %