Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - May 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 153
Report Period 1st - 31st May 2019
Issued 20 June 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
May 2019
%
Total 2019
%
Submissions
364
1557
Samples
Total (%)
28357
100.00 %
143858
100.00 %
England
23479
82.80 %
114874
79.85 %
Wales
4385
15.46 %
28125
19.55 %
Scotland
493
1.74 %
859
0.60 %
Parallel Tests
PAR-CUL-N
8515
30.03 %
29207
20.30 %
PAR-CUL-P
833
2.94 %
8208
5.71 %
PAR-HERD-S
246
0.87 %
3187
2.22 %
PAR-NEW-IN
11509
40.59 %
69859
48.56 %
PAR-OTHER
360
1.27 %
2820
1.96 %
PAR-PERSIS
6362
22.44 %
26832
18.65 %
PAR-RAPID
123
0.43 %
613
0.43 %
Total(% of all samples)
27948
98.56 %
140726
97.82 %
Serial/Extended Tests
SER-FLEXI
409
1.44 %
3115
2.17 %
SER-FRAUD
0
0.00 %
9
0.01 %
SER-NSR
0
0.00 %
8
0.01 %
SER-OTHER
0
0.00 %
Total(% of all samples)
409
1.44 %
3132
2.18 %
Total
28357
100.00 %
143858
100.00 %
Retests
Total (% of all samples)
xx-xxxx-xxxx
5.79 %
9876
6.87 %
England
1360
4.80 %
7866
5.47 %
Wales
263
0.93 %
1972
1.37 %
Scotland
18
0.06 %
38
0.03 %
Resamples
Total (% of all samples)
1217
4.29 %
8003
5.56 %
England
1013
3.57 %
6379
4.43 %
Wales
190
0.67 %
1593
1.11 %
Scotland
14
0.05 %
31
0.02 %
Rejects
Total (% of all samples)
208
0.73 %
1780
1.24 %
England
206
0.73 %
1605
1.12 %
Wales
2
0.01 %
175
0.12 %
Scotland
0
0.00 %
Table 1. Monthly Totals for May 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
8515
30.0 %
PAR-CUL-P
833
2.9 %
PAR-HERD-S
246
0.9 %
PAR-NEW-IN
11509
40.6 %
PAR-OTHER
360
1.3 %
PAR-PERSIS
6362
22.4 %
PAR-RAPID
123
0.4 %
SER-FLEXI
409
1.4 %
Total
28357
100.00 %
Figure 1. Number of samples submitted in each category during May 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Wigtown
Kirkcudbright
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Leics & Rut
I of Wight
Herts
Heref
Hampshire
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during May 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
Wigtown
Kirkcudbright
Ayrshire
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
Pembrokeshire
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
S Yorks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
17
0.06 %
0
0.00 %
14
82.35 %
3
17.65 %
3
17.65 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
4
729
2.57 %
22
3.02 %
691
94.79 %
29
3.98 %
16
2.19 %
0
0.00 %
PAR-NEW-IN
1
112
0.39 %
5
4.46 %
95
84.82 %
13
11.61 %
12
10.71 %
0
0.00 %
PAR-PERSIS
4
1,321
4.66 %
28
2.12 %
1063
80.47 %
32
2.42 %
30
2.27 %
200
15.14 %
Devon
PAR-CUL-N
27
4,553
16.06 %
258
5.67 %
4131
90.73 %
235
5.16 %
164
3.60 %
0
0.00 %
PAR-CUL-P
3
603
2.13 %
23
3.81 %
554
91.87 %
29
4.81 %
26
4.31 %
0
0.00 %
PAR-PERSIS
1
434
1.53 %
22
5.07 %
400
92.17 %
19
4.38 %
12
2.76 %
0
0.00 %
SER-FLEXI
1
19
0.07 %
2
10.53 %
16
84.21 %
2
10.53 %
1
5.26 %
0
0.00 %
Dorset
PAR-CUL-N
18
1,206
4.25 %
37
3.07 %
1103
91.46 %
88
7.30 %
66
5.47 %
0
0.00 %
SER-FLEXI
1
6
0.02 %
0
0.00 %
3
50.00 %
3
50.00 %
3
50.00 %
0
0.00 %
Gloucs
PAR-CUL-N
3
34
0.12 %
0
0.00 %
32
94.12 %
2
5.88 %
2
5.88 %
0
0.00 %
PAR-HERD-S
3
32
0.11 %
0
0.00 %
30
93.75 %
3
9.38 %
2
6.25 %
0
0.00 %
Heref
PAR-CUL-N
4
582
2.05 %
31
5.33 %
523
89.86 %
40
6.87 %
28
4.81 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
5
536
1.89 %
25
4.66 %
492
91.79 %
20
3.73 %
13
2.43 %
6
1.12 %
Shropshire
SER-FLEXI
1
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Somerset excl N
PAR-CUL-N
4
77
0.27 %
2
2.60 %
69
89.61 %
7
9.09 %
6
7.79 %
0
0.00 %
PAR-PERSIS
3
222
0.78 %
15
6.76 %
199
89.64 %
12
5.41 %
8
3.60 %
0
0.00 %
Staffs
PAR-PERSIS
2
860
3.03 %
60
6.98 %
764
88.84 %
70
8.14 %
36
4.19 %
0
0.00 %
Wiltshire
PAR-CUL-N
12
1,334
4.70 %
77
5.77 %
1183
88.68 %
94
7.05 %
74
5.55 %
0
0.00 %
PAR-CUL-P
2
230
0.81 %
1
0.43 %
225
97.83 %
11
4.78 %
4
1.74 %
0
0.00 %
PAR-NEW-IN
1
83
0.29 %
1
1.20 %
79
95.18 %
3
3.61 %
3
3.61 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
35
0.12 %
3
8.57 %
31
88.57 %
1
2.86 %
1
2.86 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
6
1,285
4.53 %
17
1.32 %
1212
94.32 %
72
5.60 %
56
4.36 %
0
0.00 %
Bucks
PAR-NEW-IN
2
38
0.13 %
4
10.53 %
29
76.32 %
5
13.16 %
5
13.16 %
0
0.00 %
Table 2a. Summary by County for May 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Cheshire
PAR-NEW-IN
21
3,115
10.98 %
62
1.99 %
2929
94.03 %
158
5.07 %
124
3.98 %
0
0.00 %
PAR-PERSIS
1
50
0.18 %
0
0.00 %
46
92.00 %
6
12.00 %
4
8.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
16
2,171
7.66 %
56
2.58 %
2026
93.32 %
122
5.62 %
89
4.10 %
0
0.00 %
E Sussex
PAR-NEW-IN
3
244
0.86 %
5
2.05 %
226
92.62 %
16
6.56 %
13
5.33 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
29
0.10 %
0
0.00 %
24
82.76 %
6
20.69 %
5
17.24 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
9
506
1.78 %
4
0.79 %
476
94.07 %
28
5.53 %
26
5.14 %
0
0.00 %
Northants
PAR-NEW-IN
5
490
1.73 %
21
4.29 %
452
92.24 %
33
6.73 %
17
3.47 %
0
0.00 %
Notts
PAR-NEW-IN
1
146
0.51 %
1
0.68 %
141
96.58 %
6
4.11 %
4
2.74 %
0
0.00 %
Oxon
PAR-NEW-IN
8
734
2.59 %
33
4.50 %
660
89.92 %
51
6.95 %
41
5.59 %
0
0.00 %
Warks
PAR-NEW-IN
6
636
2.24 %
28
4.40 %
570
89.62 %
46
7.23 %
38
5.97 %
0
0.00 %
PAR-PERSIS
1
125
0.44 %
3
2.40 %
120
96.00 %
2
1.60 %
2
1.60 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
5
469
1.65 %
16
3.41 %
416
88.70 %
46
9.81 %
37
7.89 %
0
0.00 %
Essex
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Herts
PAR-NEW-IN
2
53
0.19 %
0
0.00 %
41
77.36 %
13
24.53 %
12
22.64 %
0
0.00 %
I of Wight
PAR-NEW-IN
1
161
0.57 %
1
0.62 %
147
91.30 %
13
8.07 %
13
8.07 %
0
0.00 %
N Yorks
PAR-NEW-IN
3
192
0.68 %
1
0.52 %
175
91.15 %
20
10.42 %
16
8.33 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
197
23479
82.80 %
864
3.68 %
21396
91.13 %
1360
5.79 %
1013
4.31 %
206
0.88 %
Wales - High TB
Carms
PAR-HERD-S
3
142
0.50 %
6
4.23 %
134
94.37 %
2
1.41 %
2
1.41 %
0
0.00 %
PAR-NEW-IN
2
14
0.05 %
3
21.43 %
11
78.57 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
15
0.05 %
9
60.00 %
6
40.00 %
4
26.67 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
236
0.83 %
14
5.93 %
212
89.83 %
25
10.59 %
10
4.24 %
0
0.00 %
PAR-RAPID
9
31
0.11 %
6
19.35 %
24
77.42 %
2
6.45 %
1
3.23 %
0
0.00 %
SER-FLEXI
5
92
0.32 %
23
25.00 %
63
68.48 %
6
6.52 %
6
6.52 %
0
0.00 %
Ceredigion
PAR-NEW-IN
1
8
0.03 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-PERSIS
5
202
0.71 %
11
5.45 %
181
89.60 %
11
5.45 %
10
4.95 %
0
0.00 %
PAR-RAPID
6
20
0.07 %
4
20.00 %
16
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
8
0.03 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gwent
PAR-OTHER
1
15
0.05 %
3
20.00 %
12
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
112
0.39 %
8
7.14 %
101
90.18 %
4
3.57 %
3
2.68 %
0
0.00 %
PAR-RAPID
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
20
0.07 %
2
10.00 %
18
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
5
56
0.20 %
12
21.43 %
37
66.07 %
10
17.86 %
7
12.50 %
0
0.00 %
PAR-OTHER
2
14
0.05 %
2
14.29 %
12
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
22
1,148
4.05 %
23
2.00 %
1059
92.25 %
82
7.14 %
66
5.75 %
0
0.00 %
PAR-RAPID
8
18
0.06 %
3
16.67 %
15
83.33 %
2
11.11 %
0
0.00 %
0
0.00 %
SER-FLEXI
6
113
0.40 %
7
6.19 %
102
90.27 %
4
3.54 %
4
3.54 %
0
0.00 %
Powys
PAR-HERD-S
5
16
0.06 %
1
6.25 %
12
75.00 %
3
18.75 %
3
18.75 %
0
0.00 %
PAR-NEW-IN
5
159
0.56 %
1
0.63 %
152
95.60 %
6
3.77 %
6
3.77 %
0
0.00 %
PAR-OTHER
1
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
10
182
0.64 %
5
2.75 %
164
90.11 %
13
7.14 %
11
6.04 %
2
1.10 %
PAR-RAPID
6
11
0.04 %
1
9.09 %
10
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
32
0.11 %
1
3.13 %
30
93.75 %
1
3.13 %
1
3.13 %
0
0.00 %
S Wales
PAR-PERSIS
1
208
0.73 %
0
0.00 %
206
99.04 %
2
0.96 %
2
0.96 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
33
0.12 %
0
0.00 %
33
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
310
1.09 %
10
3.23 %
293
94.52 %
10
3.23 %
7
2.26 %
0
0.00 %
PAR-PERSIS
5
443
1.56 %
17
3.84 %
414
93.45 %
24
5.42 %
12
2.71 %
0
0.00 %
PAR-RAPID
3
7
0.02 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
Ceredigion
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
121
0.43 %
0
0.00 %
109
90.08 %
14
11.57 %
12
9.92 %
0
0.00 %
PAR-PERSIS
1
231
0.81 %
8
3.46 %
215
93.07 %
14
6.06 %
8
3.46 %
0
0.00 %
PAR-RAPID
3
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
51
0.18 %
5
9.80 %
45
88.24 %
1
1.96 %
1
1.96 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for May 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-NEW-IN
2
23
0.08 %
1
4.35 %
17
73.91 %
5
21.74 %
5
21.74 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
4
21
0.07 %
1
4.76 %
20
95.24 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
29
0.10 %
4
13.79 %
25
86.21 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
3
222
0.78 %
2
0.90 %
212
95.50 %
13
5.86 %
8
3.60 %
0
0.00 %
Wales
163
4385
15.46 %
198
4.52 %
3995
91.11 %
263
6.00 %
190
4.33 %
2
0.05 %
Scotland - Scotland
Kirkcudbright
PAR-NEW-IN
2
392
1.38 %
54
13.78 %
326
83.16 %
14
3.57 %
12
3.06 %
0
0.00 %
Wigtown
PAR-NEW-IN
2
101
0.36 %
0
0.00 %
99
98.02 %
4
3.96 %
2
1.98 %
0
0.00 %
Scotland
4
493
1.74 %
54
10.95 %
425
86.21 %
18
3.65 %
14
2.84 %
0
0.00 %
Grand Total
Sum:
364
28357
1116
25816
xx-xxxx-xxxx
1217
208
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
6
1,045
0.73 %
28
2.68 %
973
93.11 %
50
4.78 %
42
4.02 %
2
0.19 %
SER-FRAUD
1
9
0.01 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
25
2,455
1.71 %
87
3.54 %
2169
88.35 %
178
7.25 %
149
6.07 %
50
2.04 %
PAR-CUL-P
4
1,165
0.81 %
19
1.63 %
1035
88.84 %
132
11.33 %
109
9.36 %
2
0.17 %
PAR-NEW-IN
1
112
0.08 %
5
4.46 %
95
84.82 %
13
11.61 %
12
10.71 %
0
0.00 %
PAR-PERSIS
14
2,654
1.84 %
126
4.75 %
2147
80.90 %
109
4.11 %
86
3.24 %
295
11.12 %
SER-FLEXI
2
15
0.01 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
79
12,146
8.44 %
739
6.08 %
10756
88.56 %
726
5.98 %
544
4.48 %
107
0.88 %
PAR-CUL-P
20
4,525
3.15 %
286
6.32 %
3980
87.96 %
301
6.65 %
255
5.64 %
4
0.09 %
PAR-NEW-IN
3
581
0.40 %
96
16.52 %
430
74.01 %
69
11.88 %
55
9.47 %
0
0.00 %
PAR-OTHER
5
35
0.02 %
4
11.43 %
27
77.14 %
4
11.43 %
4
11.43 %
0
0.00 %
PAR-PERSIS
16
2,173
1.51 %
159
7.32 %
1882
86.61 %
170
7.82 %
131
6.03 %
1
0.05 %
SER-FLEXI
5
331
0.23 %
38
11.48 %
241
72.81 %
24
7.25 %
7
2.11 %
45
13.60 %
Dorset
PAR-CUL-N
51
5,926
4.12 %
321
5.42 %
5234
88.32 %
395
6.67 %
315
5.32 %
56
0.94 %
PAR-CUL-P
6
1,242
0.86 %
79
6.36 %
1115
89.77 %
56
4.51 %
48
3.86 %
0
0.00 %
PAR-NEW-IN
1
23
0.02 %
1
4.35 %
17
73.91 %
5
21.74 %
5
21.74 %
0
0.00 %
SER-FLEXI
2
94
0.07 %
3
3.19 %
82
87.23 %
10
10.64 %
9
9.57 %
0
0.00 %
Gloucs
PAR-CUL-N
35
2,500
1.74 %
110
4.40 %
2110
84.40 %
200
8.00 %
172
6.88 %
108
4.32 %
PAR-CUL-P
3
301
0.21 %
2
0.66 %
282
93.69 %
17
5.65 %
17
5.65 %
0
0.00 %
PAR-HERD-S
3
32
0.02 %
0
0.00 %
30
93.75 %
3
9.38 %
2
6.25 %
0
0.00 %
Heref
PAR-CUL-N
11
1,294
0.90 %
57
4.40 %
1142
88.25 %
104
8.04 %
84
6.49 %
11
0.85 %
PAR-CUL-P
1
10
0.01 %
0
0.00 %
7
70.00 %
4
40.00 %
3
30.00 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
14
1,924
1.34 %
99
5.15 %
xx-xxxx-xxxx
87.94 %
159
8.26 %
127
6.60 %
6
0.31 %
Shropshire
PAR-CUL-P
1
9
0.01 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
4
525
0.36 %
38
7.24 %
448
85.33 %
45
8.57 %
38
7.24 %
1
0.19 %
PAR-PERSIS
5
205
0.14 %
7
3.41 %
164
80.00 %
36
17.56 %
34
16.59 %
0
0.00 %
SER-FLEXI
4
556
0.39 %
21
3.78 %
508
91.37 %
34
6.12 %
27
4.86 %
0
0.00 %
Somerset excl N
PAR-CUL-N
18
2,012
1.40 %
124
6.16 %
1794
89.17 %
116
5.77 %
92
4.57 %
2
0.10 %
PAR-OTHER
2
238
0.17 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
6
841
0.58 %
72
8.56 %
618
73.48 %
42
4.99 %
33
3.92 %
118
14.03 %
Staffs
PAR-PERSIS
6
1,456
1.01 %
201
13.80 %
1173
80.56 %
121
8.31 %
83
5.70 %
0
0.00 %
Wiltshire
PAR-CUL-N
23
2,614
1.82 %
131
5.01 %
2308
88.29 %
175
6.69 %
146
5.59 %
29
1.11 %
PAR-CUL-P
5
956
0.66 %
82
8.58 %
847
88.60 %
41
4.29 %
26
2.72 %
1
0.10 %
PAR-NEW-IN
2
292
0.20 %
12
4.11 %
248
84.93 %
38
13.01 %
32
10.96 %
0
0.00 %
PAR-OTHER
2
37
0.03 %
1
2.70 %
34
91.89 %
4
10.81 %
2
5.41 %
0
0.00 %
PAR-PERSIS
2
292
0.20 %
6
2.05 %
271
92.81 %
12
4.11 %
10
3.42 %
5
1.71 %
SER-FLEXI
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
27
3,562
2.48 %
125
3.51 %
3215
90.26 %
264
7.41 %
222
6.23 %
0
0.00 %
PAR-PERSIS
3
204
0.14 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
10
803
0.56 %
35
4.36 %
699
87.05 %
53
6.60 %
54
6.72 %
15
1.87 %
Cheshire
PAR-CUL-N
1
260
0.18 %
19
7.31 %
229
88.08 %
12
4.62 %
12
4.62 %
0
0.00 %
PAR-NEW-IN
118
23,240
16.15 %
641
2.76 %
21380
92.00 %
1360
5.85 %
1069
4.60 %
150
0.65 %
PAR-PERSIS
4
526
0.37 %
3
0.57 %
504
95.82 %
22
4.18 %
19
3.61 %
0
0.00 %
Derbyshire
PAR-NEW-IN
80
7,907
5.50 %
284
3.59 %
6856
86.71 %
641
8.11 %
554
7.01 %
213
2.69 %
E Sussex
PAR-NEW-IN
14
1,651
1.15 %
82
4.97 %
1492
90.37 %
73
4.42 %
64
3.88 %
13
0.79 %
Hampshire
PAR-NEW-IN
16
1,444
1.00 %
42
2.91 %
1314
91.00 %
101
6.99 %
88
6.09 %
0
0.00 %
SER-NSR
2
8
0.01 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
41
5,146
3.58 %
223
4.33 %
4539
88.20 %
343
6.67 %
289
5.62 %
95
1.85 %
Northants
PAR-NEW-IN
19
1,609
1.12 %
107
6.65 %
1434
89.12 %
104
6.46 %
68
4.23 %
0
0.00 %
Notts
PAR-NEW-IN
7
774
0.54 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
73
6,513
4.53 %
318
4.88 %
5682
87.24 %
552
8.48 %
463
7.11 %
50
0.77 %
Warks
PAR-NEW-IN
57
5,362
3.73 %
294
5.48 %
4654
86.80 %
419
7.81 %
331
6.17 %
83
1.55 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
3
282
0.20 %
12
4.26 %
257
91.13 %
14
4.96 %
13
4.61 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
5
228
0.16 %
7
3.07 %
203
89.04 %
13
5.70 %
10
4.39 %
8
3.51 %
Cumbria
PAR-NEW-IN
8
820
0.57 %
39
4.76 %
699
85.24 %
96
11.71 %
82
10.00 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.07 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
79
0.05 %
6
7.59 %
52
65.82 %
25
31.65 %
21
26.58 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.70 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
3
628
0.44 %
16
2.55 %
550
87.58 %
68
10.83 %
61
9.71 %
1
0.16 %
Lancashire
PAR-NEW-IN
3
106
0.07 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
8
754
0.52 %
20
2.65 %
693
91.91 %
46
6.10 %
41
5.44 %
0
0.00 %
N Yorks
PAR-NEW-IN
7
875
0.61 %
22
2.51 %
766
87.54 %
71
8.11 %
59
6.74 %
28
3.20 %
SER-FLEXI
2
50
0.03 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
S Yorks
PAR-NEW-IN
2
309
0.21 %
6
1.94 %
293
94.82 %
13
4.21 %
9
2.91 %
1
0.32 %
England
915
114874
79.85 %
5383
4.69 % ######
88.36 %
7866
6.85 %
6379
5.55 %
1605
1.40 %
Wales - High TB
Carms
PAR-HERD-S
33
1,644
1.14 %
118
7.18 %
1418
86.25 %
130
7.91 %
106
6.45 %
2
0.12 %
PAR-NEW-IN
3
121
0.08 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
6
343
0.24 %
42
12.24 %
296
86.30 %
12
3.50 %
5
1.46 %
0
0.00 %
PAR-PERSIS
42
4,425
3.08 %
209
4.72 %
4011
90.64 %
260
5.88 %
194
4.38 %
13
0.29 %
PAR-RAPID
39
174
0.12 %
53
30.46 %
111
63.79 %
16
9.20 %
10
5.75 %
0
0.00 %
SER-FLEXI
22
511
0.36 %
56
10.96 %
421
82.39 %
38
7.44 %
34
6.65 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.10 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-NEW-IN
2
313
0.22 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
1
6
0.00 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
14
1,170
0.81 %
111
9.49 %
979
83.68 %
97
8.29 %
80
6.84 %
0
0.00 %
PAR-RAPID
21
53
0.04 %
15
28.30 %
34
64.15 %
5
9.43 %
4
7.55 %
0
0.00 %
SER-FLEXI
3
451
0.31 %
17
3.77 %
424
94.01 %
10
2.22 %
8
1.77 %
2
0.44 %
Gwent
PAR-HERD-S
11
887
0.62 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
1
15
0.01 %
3
20.00 %
12
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
9
571
0.40 %
29
5.08 %
507
88.79 %
42
7.36 %
34
5.95 %
1
0.18 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
19
58
0.04 %
15
25.86 %
38
65.52 %
5
8.62 %
4
6.90 %
1
1.72 %
SER-FLEXI
1
20
0.01 %
2
10.00 %
18
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembrokeshire
PAR-RAPID
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
19
234
0.16 %
31
13.25 %
185
79.06 %
25
10.68 %
19
8.12 %
0
0.00 %
PAR-OTHER
12
1,055
0.73 %
43
4.08 %
936
88.72 %
95
9.00 %
76
7.20 %
0
0.00 %
PAR-PERSIS
62
6,833
4.75 %
235
3.44 %
6165
90.22 %
517
7.57 %
429
6.28 %
4
0.06 %
PAR-RAPID
55
149
0.10 %
31
20.81 %
112
75.17 %
11
7.38 %
6
4.03 %
0
0.00 %
SER-FLEXI
15
273
0.19 %
20
7.33 %
235
86.08 %
19
6.96 %
18
6.59 %
0
0.00 %
Powys
PAR-HERD-S
12
100
0.07 %
10
10.00 %
83
83.00 %
6
6.00 %
6
6.00 %
1
1.00 %
PAR-NEW-IN
12
210
0.15 %
7
3.33 %
189
90.00 %
14
6.67 %
14
6.67 %
0
0.00 %
PAR-OTHER
1
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
23
1,045
0.73 %
55
5.26 %
931
89.09 %
70
6.70 %
56
5.36 %
3
0.29 %
PAR-RAPID
32
90
0.06 %
14
15.56 %
45
50.00 %
6
6.67 %
6
6.67 %
25
27.78 %
SER-FLEXI
7
69
0.05 %
4
5.80 %
63
91.30 %
2
2.90 %
2
2.90 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.01 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.27 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.07 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.08 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-PERSIS
1
7
0.00 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.24 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
6
558
0.39 %
29
5.20 %
514
92.11 %
27
4.84 %
15
2.69 %
0
0.00 %
PAR-RAPID
5
11
0.01 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.01 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
1
144
0.10 %
21
14.58 %
109
75.69 %
15
10.42 %
14
9.72 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
44
3,404
2.37 %
113
3.32 %
3026
88.90 %
264
7.76 %
213
6.26 %
52
1.53 %
PAR-OTHER
2
54
0.04 %
16
29.63 %
37
68.52 %
3
5.56 %
1
1.85 %
0
0.00 %
PAR-PERSIS
2
237
0.16 %
8
3.38 %
221
93.25 %
14
5.91 %
8
3.38 %
0
0.00 %
PAR-RAPID
11
39
0.03 %
8
20.51 %
30
76.92 %
1
2.56 %
1
2.56 %
0
0.00 %
SER-FLEXI
22
613
0.43 %
59
9.62 %
472
77.00 %
35
5.71 %
29
4.73 %
53
8.65 %
Powys
PAR-NEW-IN
8
591
0.41 %
18
3.05 %
532
90.02 %
44
7.45 %
41
6.94 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
5
22
0.02 %
1
4.55 %
21
95.45 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
29
0.02 %
4
13.79 %
25
86.21 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.01 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
7
160
0.11 %
8
5.00 %
146
91.25 %
7
4.38 %
5
3.13 %
1
0.63 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
8
274
0.19 %
2
0.73 %
249
90.88 %
14
5.11 %
8
2.92 %
15
5.47 %
Wales
635
28125
19.55 %
1546
5.50 %
24814
88.23 %
1972
7.01 %
1593
5.66 %
175
0.62 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
366
0.25 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
2
392
0.27 %
54
13.78 %
326
83.16 %
14
3.57 %
12
3.06 %
0
0.00 %
Wigtown
PAR-NEW-IN
2
101
0.07 %
0
0.00 %
99
98.02 %
4
3.96 %
2
1.98 %
0
0.00 %
Scotland
7
859
0.60 %
58
6.75 %
770
89.64 %
38
4.42 %
31
3.61 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
6987
######
9876
8003
1780
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol May 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
193
23448
99.9%
862
3.7%
21372
91.1%
1354
5.8%
1008
4.3%
206
0.9%
Serial/Extended
4
31
0.1%
2
6.5%
24
77.4%
6
19.4%
5
16.1%
0
0.0 %
Total
197
23479
100.0%
864
3.7%
21396
91.1%
1360
5.8%
1013
4.3%
206
0.9%
Scotland
Parallel
4
493
100.0%
54
11.0%
425
86.2%
18
3.7%
14
2.8%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
4
493
100.0%
54
11.0%
425
86.2%
18
3.7%
14
2.8%
0
0.0 %
Wales
Parallel
141
4007
91.4%
156
3.9%
3673
91.7%
249
6.2%
176
4.4%
2
0.0%
Serial/Extended
22
378
8.6%
42
11.1%
322
85.2%
14
3.7%
14
3.7%
0
0.0 %
Total
163
4385
100.0%
198
4.5%
3995
91.1%
263
6.0%
190
4.3%
2
0.0%
GB
Parallel
338
27948
98.6%
1072
3.8%
25470
91.1%
1621
5.8%
1198
4.3%
208
0.7%
Serial/Extended
26
409
1.4%
44
10.8%
346
84.6%
20
4.9%
19
4.6%
0
0.0 %
Total
364
28357
100.0%
1116
3.9%
25816
91.0%
xx-xxxx-xxxx
5.8%
1217
4.3%
208
0.7%
197.00
163.00
4.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
896 113809
99.1%
5320
4.7% 100596
88.4%
7794
6.8%
6334
5.6%
1560
1.4%
Serial/Extended
19
1065
0.9%
63
5.9%
912
85.6%
72
6.8%
45
4.2%
45
4.2%
Total
915 114874
100.0%
5383
4.7% 101508
88.4%
7866
6.8%
6379
5.6%
1605
1.4%
Scotland
Parallel
7
859
100.0%
58
6.8%
770
89.6%
38
4.4%
31
3.6%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
7
859
100.0%
58
6.8%
770
89.6%
38
4.4%
31
3.6%
0
0.0 %
Wales
Parallel
561
26058
92.7%
1379
5.3%
23064
88.5%
1863
7.1%
1498
5.7%
120
0.5%
Serial/Extended
74
2067
7.3%
167
8.1%
1750
84.7%
109
5.3%
95
4.6%
55
2.7%
Total
635
28125
100.0%
1546
5.5%
24814
88.2%
1972
7.0%
1593
5.7%
175
0.6%
GB
Parallel
1464 140726
97.8%
6757
4.8% 124430
88.4%
9695
6.9%
7863
5.6%
xx-xxxx-xxxx
1.2%
Serial/Extended
93
3132
2.2%
230
7.3%
2662
85.0%
181
5.8%
140
4.5%
100
3.2%
Total
xx-xxxx-xxxx
100.0%
6987
4.9% 127092
88.3%
9876
6.9%
8003
5.6%
1780
1.2%
915.00
635.00
7.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
26
409
1.4%
44
10.8%
346
84.6%
20
4.9 %
19
4.6 %
0
Parallel - Herd or Group Slaughter
16
246
0.9%
19
7.7%
213
86.6%
18
7.3 %
14
5.7 %
0
Parallel New Infection
117
11509
40.6%
316
2.7%
10635
92.4%
709
6.2 %
558
4.8 %
0
Parallel - new infection cull area
72
8515
30.0%
427
5.0%
7732
90.8%
495
5.8 %
356
4.2 %
0
Parallel - Other
10
360
1.3%
25
6.9%
328
91.1%
14
3.9 %
7
1.9 %
0
Parallel Persistent Infection
70
6362
22.4%
242
3.8%
5681
89.3%
340
5.3 %
231
3.6 %
208
3.3 %
Parallel - persistent infection cull area
5
833
2.9%
24
2.9%
779
93.5%
40
4.8 %
30
3.6 %
0
Parallel - Rapid Testing Two-times IRs
48
123
0.4%
19
15.4%
102
82.9%
5
4.1 %
2
1.6 %
0
Total
364
28357
100.0%
1116
3.9%
25816
91.0%
xx-xxxx-xxxx
5.8 %
1217
4.3 %
208
0.7 %
Table 4a. Summaryof IFN Gamma results by submission reason (May 2019)
England - HRA
Extended Flexible IFN-GAMMA test
3
30
0.1%
2
6.7%
23
76.7%
6
20.0 %
5
16.7 %
0
Parallel - Herd or Group Slaughter
3
32
0.1%
0
0.0 %
30
93.8%
3
9.4 %
2
6.3 %
0
Parallel New Infection
2
195
0.7%
6
3.1%
174
89.2%
16
8.2 %
15
7.7 %
0
Parallel - new infection cull area
72
8515
30.0%
427
5.0%
7732
90.8%
495
5.8 %
356
4.2 %
0
Parallel - Other
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel Persistent Infection
17
3425
12.1%
153
4.5%
2963
86.5%
157
4.6 %
103
3.0 %
206
6.0 %
Parallel - persistent infection cull area
5
833
2.9%
24
2.9%
779
93.5%
40
4.8 %
30
3.6 %
0
England - HRA
104
13032
46.0%
612
4.7%
11703
89.8%
717
5.5 %
511
3.9 %
206
1.6 %
England - Edge
Parallel New Infection
78
9394
33.1%
231
2.5%
8745
93.1%
543
5.8 %
418
4.4 %
0
Parallel Persistent Infection
2
175
0.6%
3
1.7%
166
94.9%
8
4.6 %
6
3.4 %
0
England - Edge
80
9569
33.7%
234
2.4%
8911
93.1%
551
5.8 %
424
4.4 %
0
England - LRA
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
12
877
3.1%
18
2.1%
781
89.1%
92
10.5 %
78
8.9 %
0
England - LRA
13
878
3.1%
18
2.1%
782
89.1%
92
10.5 %
78
8.9 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
18
298
1.1%
33
11.1%
252
84.6%
13
4.4 %
13
4.4 %
0
Parallel - Herd or Group Slaughter
13
214
0.8%
19
8.9%
183
85.5%
15
7.0 %
12
5.6 %
0
Parallel New Infection
8
181
0.6%
4
2.2%
169
93.4%
8
4.4 %
8
4.4 %
0
Parallel - Other
6
48
0.2%
15
31.3%
33
68.8%
4
8.3 %
0
0
Parallel Persistent Infection
45
2088
7.4%
61
2.9%
1923
92.1%
137
6.6 %
102
4.9 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
34
88
0.3%
15
17.0%
72
81.8%
4
4.5 %
1
1.1 %
0
Wales - High TB
124
2917
10.3%
147
5.0%
2632
90.2%
181
6.2 %
136
4.7 %
2
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
80
0.3%
9
11.3%
70
87.5%
1
1.3 %
1
1.3 %
0
Parallel New Infection
10
147
0.5%
1
0.7%
129
87.8%
19
12.9 %
17
11.6 %
0
Parallel - Other
2
310
1.1%
10
3.2%
293
94.5%
10
3.2 %
7
2.3 %
0
Parallel Persistent Infection
6
674
2.4%
25
3.7%
629
93.3%
38
5.6 %
20
3.0 %
0
Parallel - Rapid Testing Two-times IRs
13
34
0.1%
4
11.8%
29
85.3%
1
2.9 %
1
2.9 %
0
Wales - Intermediate TB
35
1245
4.4%
49
3.9%
1150
92.4%
69
5.5 %
46
3.7 %
0
Wales - Low TB
Parallel New Infection
3
222
0.8%
2
0.9%
212
95.5%
13
5.9 %
8
3.6 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
4
223
0.8%
2
0.9%
213
95.5%
13
5.8 %
8
3.6 %
0
Scotland - Scotland
Parallel New Infection
4
493
1.7%
54
11.0%
425
86.2%
18
3.7 %
14
2.8 %
0
Scotland - Scotland
4
493
1.7%
54
11.0%
425
86.2%
18
3.7 %
14
2.8 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
90
3115
2.2%
230
7.4%
2645
84.9%
181
5.8 %
140
4.5 %
100
3.2 %
Parallel - Herd or Group Slaughter
88
3187
2.2%
231
7.2%
2720
85.3%
278
8.7 %
232
7.3 %
5
0.2 %
Parallel New Infection
609
69859
48.6%
2710
3.9%
62302
89.2%
4935
7.1 %
4058
5.8 %
789
1.1 %
Parallel - new infection cull area
243
29207
20.3%
1588
5.4%
25742
88.1%
1906
6.5 %
1514
5.2 %
363
1.2 %
Parallel - Other
46
2820
2.0%
218
7.7%
2399
85.1%
202
7.2 %
161
5.7 %
42
1.5 %
Parallel Persistent Infection
241
26832
18.7%
1399
5.2%
23581
87.9%
1777
6.6 %
1407
5.2 %
448
1.7 %
Parallel - persistent infection cull area
40
8208
5.7%
469
5.7%
7274
88.6%
551
6.7 %
458
5.6 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
197
613
0.4%
142
23.2%
412
67.2%
46
7.5 %
33
5.4 %
26
4.2 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
6987
4.9%
127092
88.3%
9876
6.9 %
8003
5.6 %
1780
1.2 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
14
998
0.7%
62
6.2%
847
84.9%
69
6.9 %
44
4.4 %
45
4.5 %
Parallel - Herd or Group Slaughter
3
32
0.0%
0
0.0 %
30
93.8%
3
9.4 %
2
6.3 %
0
Parallel New Infection
8
1009
0.7%
114
11.3%
791
78.4%
125
12.4 %
104
10.3 %
0
Parallel - new infection cull area
242
28947
20.1%
1569
5.4%
25513
88.1%
1894
6.5 %
1502
5.2 %
363
1.3 %
Parallel - Other
14
836
0.6%
77
9.2%
665
79.5%
61
7.3 %
52
6.2 %
42
5.0 %
Parallel Persistent Infection
69
10590
7.4%
698
6.6%
8920
84.2%
699
6.6 %
546
5.2 %
427
4.0 %
Parallel - persistent infection cull area
40
8208
5.7%
469
5.7%
7274
88.6%
551
6.7 %
458
5.6 %
7
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - Edge
Parallel New Infection
462
58011
40.3%
2187
3.8%
51883
89.4%
3979
6.9 %
3258
5.6 %
683
1.2 %
Parallel - new infection cull area
1
260
0.2%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
10
1012
0.7%
20
2.0%
957
94.6%
41
4.1 %
35
3.5 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel New Infection
47
4904
3.4%
167
3.4%
4334
88.4%
429
8.7 %
365
7.4 %
38
0.8 %
England
915 114874
79.9%
5383
4.7%
101508
88.4%
7866
6.8 %
6379
5.6 %
1605
1.4 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Wales - High TB
Extended Flexible IFN-GAMMA test
51
1425
1.0%
104
7.3%
1253
87.9%
74
5.2 %
66
4.6 %
2
0.1 %
Parallel - Herd or Group Slaughter
81
3129
2.2%
228
7.3%
2677
85.6%
265
8.5 %
220
7.0 %
5
0.2 %
Parallel New Infection
17
644
0.4%
43
6.7%
568
88.2%
35
5.4 %
33
5.1 %
0
Parallel - Other
22
1434
1.0%
90
6.3%
1263
88.1%
107
7.5 %
81
5.6 %
0
Parallel Persistent Infection
154
14435
10.0%
644
4.5%
12969
89.8%
996
6.9 %
803
5.6 %
21
0.1 %
Parallel - Rapid Testing Two-times IRs
171
535
0.4%
129
24.1%
349
65.2%
44
8.2 %
31
5.8 %
26
4.9 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
23
642
0.4%
63
9.8%
497
77.4%
35
5.5 %
29
4.5 %
53
8.3 %
Parallel - Herd or Group Slaughter
4
26
0.0%
3
11.5%
13
50.0%
10
38.5 %
10
38.5 %
0
Parallel New Infection
53
3998
2.8%
131
3.3%
3561
89.1%
308
7.7 %
254
6.4 %
52
1.3 %
Parallel - Other
10
550
0.4%
51
9.3%
471
85.6%
34
6.2 %
28
5.1 %
0
Parallel Persistent Infection
8
795
0.6%
37
4.7%
735
92.5%
41
5.2 %
23
2.9 %
0
Parallel - Rapid Testing Two-times IRs
25
77
0.1%
13
16.9%
62
80.5%
2
2.6 %
2
2.6 %
0
Wales - Low TB
Parallel New Infection
15
434
0.3%
10
2.3%
395
91.0%
21
4.8 %
13
3.0 %
16
3.7 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
635
28125
19.6%
1546
5.5%
24814
88.2%
1972
7.0 %
1593
5.7 %
175
0.6 %
Scotland - Scotland
Parallel New Infection
7
859
0.6%
58
6.8%
770
89.6%
38
4.4 %
31
3.6 %
0
Scotland
7
859
0.6%
58
6.8%
770
89.6%
38
4.4 %
31
3.6 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 June
187
18891
110
701
2
2
0
0
0
0
78
2
0
12
0
579
0
25
0
0
July
207
24111
119
806
1
12
0
0
0
0
67
4
0
2
0
675
3
38
0
2
August
228
19599
120
682
4
3
0
0
0
0
64
2
0
8
1
569
0
31
0
0
September
216
21441
137
946
4
6
0
0
0
0
33
1
0
2
0
842
0
51
1
4
October
274
23976
152
1417
1
8
0
0
0
0
100
2
0
3
0
1242
0
44
0
2
November
282
24570
192
1580
2
4
0
0
0
0
42
5
0
8
2
1336
0
169
0
10
December
228
21554
147
1241
6
8
0
0
0
0
92
2
0
7
1
1037
0
82
0
5
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
4
0
9
1
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
1
4
0
1316
15
70
0
4
April
269
30320
162
1135
1
8
0
0
0
0
87
0
5
3
0
989
26
14
0
0
May
364
28357
176
1116
96
0
0
0
0
89
0
4
0
0
881
46
0
0
0
Totals (last
12 months)
3179
298000
1909
14360
28
159
0
0
0
0
1039
25
10
61
5
12217
90
661
3
31
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1831
234237
1229
10796
112
0
0
0
0
863
16
4
36
3
9611
31
79
0
2
Scotland
16
1309
8
80
8
0
0
0
0
6
0
3
0
0
60
0
3
0
0
Wales
1332
62454
672
3484
39
0
0
0
0
170
9
3
25
2
2546
59
579
3
29
Totals
3179
298000
1909
14360
159
0
0
0
0
1039
25
10
61
5
12217
90
661
3
31
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
0
5000
10000
15000
20000
25000
30000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
35
40
45
50
Wiltshire
W Glamorgan
S Wales
Somerset excl N
S Glamorgan
Pembs
N Yorks
Northants
Leics & Rut
I of Wight
Heref
Gwynedd
Gloucs
Essex
Devon
Cumbria
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during May 2019 (% submission per County)
County
%Resampled %Retested
Avon
17.65 %
17.65 %
Berks
4.36 %
5.60 %
Bucks
13.16 %
13.16 %
Carms
3.02 %
5.72 %
Ceredigion
5.86 %
6.28 %
Cheshire
4.04 %
5.18 %
Corn & Scilly
2.68 %
3.42 %
Cumbria
7.89 %
9.81 %
Derbyshire
4.10 %
5.62 %
Devon
3.62 %
5.08 %
Dorset
5.69 %
7.51 %
Essex
E Sussex
5.33 %
6.56 %
Gloucs
6.06 %
7.58 %
Gwent
1.99 %
2.65 %
Gwynedd
Hampshire
17.24 %
20.69 %
Heref
3.66 %
5.36 %
Herts
22.64 %
24.53 %
I of Wight
8.07 %
8.07 %
Kirkcudbright
3.06 %
3.57 %
Leics & Rut
5.14 %
5.53 %
NE Wales
4.62 %
6.69 %
Northants
3.47 %
6.73 %
Notts
2.74 %
4.11 %
N Yorks
8.29 %
10.36 %
Oxon
5.59 %
6.95 %
Pembs
5.71 %
7.26 %
Powys
6.07 %
6.54 %
S Glamorgan
Shropshire
20.00 %
20.00 %
Somerset excl
4.68 %
6.35 %
Staffs
4.19 %
8.14 %
S Wales
0.82 %
0.82 %
Warks
5.26 %
6.31 %

W Glamorgan
Wigtown
1.98 %
3.96 %
Wiltshire
4.87 %
6.48 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
23289
864
3.7%
23479
864
3.7%
Wales
4302
198
4.6%
4385
198
4.5%
Scotland
493
54
11.0%
493
54
11.0%
GB
28083
1116
4.0%
28357
1116
3.9%
Table 6a (i): Number of animals and submissions tested in May 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
108872
5383
4.9%
114874
5383
4.7%
Wales
26100
1546
5.9%
28125
1546
5.5%
Scotland
859
58
6.8%
859
58
6.8%
GB
135830
6987
5.1%
143858
6987
4.9%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of May
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
16310
837
5.1%
16507
837
5.1%
Wales
3910
85
2.2%
4021
85
2.1%
Scotland
522
25
4.8%
538
25
4.6%
GB
20742
947
4.6%
21066
947
4.5%
Table 6a (ii): Number of animals and submissions tested in May 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of May
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
69959
4207
6.0%
74275
4207
5.7%
Wales
21403
931
4.3%
23278
931
4.0%
Scotland
xx-xxxx-xxxx
159
9.4%
1791
159
8.9%
GB
93040
5297
5.7%
99344
5297
5.3%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
1
6
0
0
Herefordshire
PRV-SP
1
2
0
0
Total
2
8
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples May 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
1
6
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Somerset excl North
PRV-SE
4
41
15
36.59 %
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
9
61
15
24.59 %
Isle of Man
SER-IOM
13
52
1
1.92 %

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
2
74
11
14.9%
Ceredigion
1
29
7
24.1%
Pembrokeshire
3
102
4
3.9%
Powys
1
6
1
16.7%
South Wales
1
37
1
2.7%
TC0077
Sum:
248
24
9.7 %
Table 8a Number of Cattle Antibody Tests May 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
4
199
15
7.5%
Ceredigion
1
29
7
24.1%
North East Wales
11
383
63
16.4%
Pembrokeshire
5
166
5
3.0%
Powys
1
6
1
16.7%
South Wales
3
155
14
9.0%
TC0077
Sum:
938
105
11.2 %
Table 8b Number of Cattle Antibody Tests 2019