Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - April 2022
Source: environment.data.gov.uk (2023-01-25)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2022. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 188
Report Period 1st - 30th April 2022
Issued 10 May 2022

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
April 2022
%
Total 2022
%
Submissions
220
979
Samples
Total (%)
15067
100.00 %
74874
100.00 %
England
9857
65.42 %
58596
78.26 %
Wales
4738
31.45 %
15801
21.10 %
Scotland
472
3.13 %
477
0.64 %
Parallel Tests
PAR-CHR
9
0.06 %
710
0.95 %
PAR-CUL-N
863
5.73 %
6791
9.07 %
PAR-CUL-P
0
0.00 %
3533
4.72 %
PAR-HERD-S
0
0.00 %
40
0.05 %
PAR-IR-EX
48
0.32 %
134
0.18 %
PAR-IR-SEV
176
1.17 %
673
0.90 %
PAR-NEW-IN
4700
31.19 %
14454
19.30 %
PAR-OTHER
254
1.69 %
xx-xxxx-xxxx
2.27 %
PAR-PERSIS
1021
6.78 %
10073
13.45 %
PAR-POSTM
56
0.37 %
190
0.25 %
PAR-RAPID
59
0.39 %
323
0.43 %
PAR-RECUR
5945
39.46 %
30235
40.38 %
Total(% of all samples)
13131
87.15 %
68853
91.96 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
1936
12.85 %
6015
8.03 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.01 %
SER-OTHER
0
0.00 %
2
0.00 %
Total(% of all samples)
1936
12.85 %
6021
8.04 %
Total
15067
100.00 %
74874
100.00 %
Retests
Total (% of all samples)
1168
7.75 %
6305
8.42 %
England
791
5.25 %
4922
6.57 %
Wales
345
2.29 %
1351
1.80 %
Scotland
32
0.21 %
32
0.04 %
Resamples
Total (% of all samples)
978
6.49 %
5371
7.17 %
England
656
4.35 %
4202
5.61 %
Wales
297
1.97 %
1144
1.53 %
Scotland
25
0.17 %
25
0.03 %
Rejects
Total (% of all samples)
23
0.15 %
628
0.84 %
England
11
0.07 %
512
0.68 %
Wales
12
0.08 %
116
0.15 %
Scotland
0
0.00 %
Table 1. Monthly Totals for April 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
9
0.1 %
PAR-CUL-N
863
5.7 %
PAR-IR-EX
48
0.3 %
PAR-IR-SEV
176
1.2 %
PAR-NEW-IN
4700
31.2 %
PAR-OTHER
254
1.7 %
PAR-PERSIS
1021
6.8 %
PAR-POSTM
56
0.4 %
PAR-RAPID
59
0.4 %
PAR-RECUR
5945
39.5 %
SER-FLEXI
1936
12.8 %
Total
15067
100.00 %
Figure 1. Number of samples submitted in each category during April 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
200
400
600
800
1000
1200
1400
1600
1800
Wigtown
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Worcs
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Leics & Rut
Kent
Heref
Hampshire
Gr Manchester
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Beds
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during April 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
Wigtown
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Worcs
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Leics & Rut
Lancashire
Kent
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
1
9
0.06 %
0
0.00 %
5
55.56 %
5
55.56 %
4
44.44 %
0
0.00 %
PAR-PERSIS
1
327
2.17 %
20
6.12 %
291
88.99 %
20
6.12 %
16
4.89 %
0
0.00 %
PAR-RECUR
2
235
1.56 %
7
2.98 %
204
86.81 %
30
12.77 %
24
10.21 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
3
255
1.69 %
34
13.33 %
213
83.53 %
10
3.92 %
8
3.14 %
0
0.00 %
Devon
PAR-PERSIS
1
23
0.15 %
1
4.35 %
17
73.91 %
5
21.74 %
5
21.74 %
0
0.00 %
PAR-RECUR
17
1,620
10.75 %
73
4.51 %
1382
85.31 %
175
10.80 %
164
10.12 %
1
0.06 %
Dorset
PAR-OTHER
1
21
0.14 %
0
0.00 %
20
95.24 %
1
4.76 %
1
4.76 %
0
0.00 %
PAR-RECUR
2
273
1.81 %
22
8.06 %
237
86.81 %
22
8.06 %
14
5.13 %
0
0.00 %
Gloucs
PAR-CUL-N
1
127
0.84 %
13
10.24 %
94
74.02 %
18
14.17 %
18
14.17 %
2
1.57 %
PAR-RECUR
3
711
4.72 %
14
1.97 %
654
91.98 %
53
7.45 %
43
6.05 %
0
0.00 %
Heref
PAR-OTHER
1
90
0.60 %
7
7.78 %
81
90.00 %
6
6.67 %
2
2.22 %
0
0.00 %
PAR-RECUR
9
858
5.69 %
16
1.86 %
790
92.07 %
63
7.34 %
52
6.06 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
280
1.86 %
21
7.50 %
245
87.50 %
21
7.50 %
14
5.00 %
0
0.00 %
PAR-PERSIS
3
247
1.64 %
8
3.24 %
221
89.47 %
21
8.50 %
18
7.29 %
0
0.00 %
PAR-RECUR
2
325
2.16 %
7
2.15 %
305
93.85 %
17
5.23 %
13
4.00 %
0
0.00 %
Somerset excl N
PAR-PERSIS
2
22
0.15 %
1
4.55 %
15
68.18 %
7
31.82 %
6
27.27 %
0
0.00 %
PAR-RECUR
6
622
4.13 %
46
7.40 %
554
89.07 %
28
4.50 %
22
3.54 %
0
0.00 %
SER-FLEXI
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Staffs
PAR-OTHER
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
62
0.41 %
3
4.84 %
41
66.13 %
19
30.65 %
18
29.03 %
0
0.00 %
Wiltshire
PAR-OTHER
1
4
0.03 %
1
25.00 %
3
75.00 %
2
50.00 %
0
0.00 %
0
0.00 %
Worcs
PAR-RECUR
2
33
0.22 %
6
18.18 %
24
72.73 %
3
9.09 %
3
9.09 %
0
0.00 %
England - Edge
Bucks
PAR-NEW-IN
2
504
3.35 %
6
1.19 %
480
95.24 %
23
4.56 %
18
3.57 %
0
0.00 %
Cheshire
PAR-NEW-IN
1
143
0.95 %
3
2.10 %
132
92.31 %
9
6.29 %
8
5.59 %
0
0.00 %
PAR-RECUR
2
282
1.87 %
5
1.77 %
267
94.68 %
17
6.03 %
10
3.55 %
0
0.00 %
Derbyshire
PAR-RECUR
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2a. Summary by County for April 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-NEW-IN
2
448
2.97 %
15
3.35 %
400
89.29 %
33
7.37 %
30
6.70 %
3
0.67 %
Leics & Rut
PAR-CUL-N
1
30
0.20 %
3
10.00 %
26
86.67 %
2
6.67 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
2
575
3.82 %
5
0.87 %
530
92.17 %
43
7.48 %
36
6.26 %
4
0.70 %
Notts
PAR-CUL-N
2
451
2.99 %
9
2.00 %
422
93.57 %
31
6.87 %
19
4.21 %
1
0.22 %
PAR-NEW-IN
3
354
2.35 %
9
2.54 %
326
92.09 %
25
7.06 %
19
5.37 %
0
0.00 %
Oxon
PAR-NEW-IN
1
195
1.29 %
15
7.69 %
164
84.10 %
17
8.72 %
16
8.21 %
0
0.00 %
PAR-RECUR
1
121
0.80 %
3
2.48 %
112
92.56 %
7
5.79 %
6
4.96 %
0
0.00 %
Warks
PAR-RECUR
3
308
2.04 %
10
3.25 %
268
87.01 %
33
10.71 %
30
9.74 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
1
72
0.48 %
1
1.39 %
67
93.06 %
6
8.33 %
4
5.56 %
0
0.00 %
Kent
PAR-NEW-IN
1
63
0.42 %
2
3.17 %
56
88.89 %
5
7.94 %
5
7.94 %
0
0.00 %
N Yorks
PAR-NEW-IN
1
8
0.05 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
2
152
1.01 %
5
3.29 %
141
92.76 %
11
7.24 %
6
3.95 %
0
0.00 %
England
91
9857
65.42 %
391
3.97 %
8799
89.27 %
791
8.02 %
656
6.66 %
11
0.11 %
Wales - High TB
Carms
PAR-IR-EX
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
3
0.02 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
10
0.07 %
2
20.00 %
7
70.00 %
1
10.00 %
1
10.00 %
0
0.00 %
SER-FLEXI
1
271
1.80 %
27
9.96 %
238
87.82 %
11
4.06 %
6
2.21 %
0
0.00 %
Ceredigion
PAR-IR-EX
2
9
0.06 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-SEV
4
21
0.14 %
3
14.29 %
16
76.19 %
2
9.52 %
2
9.52 %
0
0.00 %
PAR-OTHER
2
138
0.92 %
1
0.72 %
124
89.86 %
17
12.32 %
13
9.42 %
0
0.00 %
PAR-RAPID
3
5
0.03 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
6
721
4.79 %
34
4.72 %
643
89.18 %
44
6.10 %
42
5.83 %
2
0.28 %
Pembs
PAR-IR-EX
4
24
0.16 %
11
45.83 %
13
54.17 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
15
96
0.64 %
23
23.96 %
70
72.92 %
4
4.17 %
3
3.13 %
0
0.00 %
PAR-PERSIS
5
180
1.19 %
8
4.44 %
150
83.33 %
31
17.22 %
22
12.22 %
0
0.00 %
PAR-RAPID
10
30
0.20 %
9
30.00 %
18
60.00 %
3
10.00 %
3
10.00 %
0
0.00 %
PAR-RECUR
1
347
2.30 %
10
2.88 %
320
92.22 %
17
4.90 %
15
4.32 %
2
0.58 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
7
938
6.23 %
56
5.97 %
823
87.74 %
60
6.40 %
54
5.76 %
5
0.53 %
Powys
PAR-IR-SEV
4
17
0.11 %
6
35.29 %
11
64.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
222
1.47 %
0
0.00 %
212
95.50 %
11
4.95 %
10
4.50 %
0
0.00 %
PAR-RAPID
3
4
0.03 %
2
50.00 %
0
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RECUR
1
146
0.97 %
9
6.16 %
129
88.36 %
8
5.48 %
8
5.48 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
2
7
0.05 %
3
42.86 %
2
28.57 %
2
28.57 %
2
28.57 %
0
0.00 %
SER-FLEXI
1
5
0.03 %
1
20.00 %
2
40.00 %
2
40.00 %
2
40.00 %
0
0.00 %
Ceredigion
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
13
0.09 %
3
23.08 %
10
76.92 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
31
0.21 %
5
16.13 %
26
83.87 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
7
403
2.67 %
10
2.48 %
351
87.10 %
46
11.41 %
41
10.17 %
1
0.25 %
PAR-POSTM
7
26
0.17 %
2
7.69 %
24
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
10
0.07 %
1
10.00 %
9
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
4
252
1.67 %
26
10.32 %
210
83.33 %
20
7.94 %
16
6.35 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
8
682
4.53 %
18
2.64 %
612
89.74 %
59
8.65 %
50
7.33 %
2
0.29 %
PAR-POSTM
5
24
0.16 %
4
16.67 %
20
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
1
94
0.62 %
6
6.38 %
83
88.30 %
5
5.32 %
5
5.32 %
0
0.00 %
PAR-POSTM
3
6
0.04 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
127
4738
31.45 %
284
5.99 %
4145
87.48 %
345
7.28 %
297
6.27 %
12
0.25 %
Scotland - Scotland
Wigtown
PAR-NEW-IN
2
472
3.13 %
12
2.54 %
435
92.16 %
32
6.78 %
25
5.30 %
0
0.00 %
Scotland
2
472
3.13 %
12
2.54 %
435
92.16 %
32
6.78 %
25
5.30 %
0
0.00 %
Grand Total
Sum:
220
15067
687
13379
1168
978
23
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
2
134
0.18 %
10
7.46 %
111
82.84 %
15
11.19 %
13
9.70 %
0
0.00 %
PAR-CUL-N
3
527
0.70 %
26
4.93 %
485
92.03 %
16
3.04 %
15
2.85 %
1
0.19 %
PAR-CUL-P
2
222
0.30 %
20
9.01 %
173
77.93 %
37
16.67 %
29
13.06 %
0
0.00 %
PAR-PERSIS
1
327
0.44 %
20
6.12 %
291
88.99 %
20
6.12 %
16
4.89 %
0
0.00 %
PAR-RECUR
9
1,144
1.53 %
51
4.46 %
1009
88.20 %
93
8.13 %
78
6.82 %
6
0.52 %
Corn & Scilly
PAR-CUL-N
5
418
0.56 %
48
11.48 %
359
85.89 %
17
4.07 %
11
2.63 %
0
0.00 %
PAR-CUL-P
5
684
0.91 %
27
3.95 %
609
89.04 %
54
7.89 %
48
7.02 %
0
0.00 %
PAR-OTHER
1
102
0.14 %
1
0.98 %
98
96.08 %
4
3.92 %
3
2.94 %
0
0.00 %
PAR-PERSIS
8
1,683
2.25 %
73
4.34 %
1491
88.59 %
137
8.14 %
119
7.07 %
0
0.00 %
PAR-RECUR
22
3,229
4.31 %
156
4.83 %
2760
85.48 %
357
11.06 %
312
9.66 %
1
0.03 %
Devon
PAR-CUL-N
9
1,462
1.95 %
103
7.05 %
1222
83.58 %
152
10.40 %
136
9.30 %
1
0.07 %
PAR-CUL-P
6
1,195
1.60 %
47
3.93 %
1049
87.78 %
114
9.54 %
94
7.87 %
5
0.42 %
PAR-PERSIS
8
832
1.11 %
56
6.73 %
708
85.10 %
91
10.94 %
68
8.17 %
0
0.00 %
PAR-RECUR
61
7,005
9.36 %
411
5.87 %
5996
85.60 %
660
9.42 %
586
8.37 %
12
0.17 %
Dorset
PAR-CUL-N
5
1,423
1.90 %
58
4.08 %
1279
89.88 %
100
7.03 %
83
5.83 %
3
0.21 %
PAR-OTHER
3
70
0.09 %
1
1.43 %
67
95.71 %
2
2.86 %
2
2.86 %
0
0.00 %
PAR-RECUR
6
1,241
1.66 %
61
4.92 %
1100
88.64 %
93
7.49 %
80
6.45 %
0
0.00 %
SER-FLEXI
2
43
0.06 %
7
16.28 %
34
79.07 %
2
4.65 %
2
4.65 %
0
0.00 %
Gloucs
PAR-CUL-N
7
320
0.43 %
23
7.19 %
247
77.19 %
51
15.94 %
47
14.69 %
3
0.94 %
PAR-CUL-P
1
195
0.26 %
7
3.59 %
170
87.18 %
17
8.72 %
17
8.72 %
1
0.51 %
PAR-RECUR
10
2,522
3.37 %
52
2.06 %
2328
92.31 %
165
6.54 %
141
5.59 %
1
0.04 %
Heref
PAR-OTHER
4
512
0.68 %
29
5.66 %
462
90.23 %
35
6.84 %
21
4.10 %
0
0.00 %
PAR-PERSIS
8
1,130
1.51 %
61
5.40 %
973
86.11 %
104
9.20 %
93
8.23 %
3
0.27 %
PAR-RECUR
23
3,153
4.21 %
101
3.20 %
2874
91.15 %
220
6.98 %
177
5.61 %
1
0.03 %
Shropshire
PAR-CHR
2
576
0.77 %
6
1.04 %
536
93.06 %
38
6.60 %
34
5.90 %
0
0.00 %
PAR-NEW-IN
1
280
0.37 %
21
7.50 %
245
87.50 %
21
7.50 %
14
5.00 %
0
0.00 %
Table 2b. Summary by County for 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
3
246
0.33 %
4
1.63 %
221
89.84 %
22
8.94 %
21
8.54 %
0
0.00 %
PAR-PERSIS
7
1,823
2.43 %
70
3.84 %
1629
89.36 %
153
8.39 %
124
6.80 %
0
0.00 %
PAR-RECUR
31
3,722
4.97 %
79
2.12 %
3377
90.73 %
291
7.82 %
265
7.12 %
1
0.03 %
Somerset excl N
PAR-OTHER
3
87
0.12 %
15
17.24 %
65
74.71 %
8
9.20 %
7
8.05 %
0
0.00 %
PAR-PERSIS
3
239
0.32 %
4
1.67 %
215
89.96 %
24
10.04 %
20
8.37 %
0
0.00 %
PAR-RECUR
18
2,325
3.11 %
141
6.06 %
2049
88.13 %
175
7.53 %
130
5.59 %
5
0.22 %
SER-FLEXI
2
27
0.04 %
5
18.52 %
19
70.37 %
4
14.81 %
3
11.11 %
0
0.00 %
Staffs
PAR-CUL-N
2
263
0.35 %
6
2.28 %
214
81.37 %
43
16.35 %
43
16.35 %
0
0.00 %
PAR-CUL-P
1
425
0.57 %
11
2.59 %
401
94.35 %
17
4.00 %
13
3.06 %
0
0.00 %
PAR-OTHER
2
50
0.07 %
3
6.00 %
46
92.00 %
1
2.00 %
1
2.00 %
0
0.00 %
PAR-PERSIS
4
739
0.99 %
13
1.76 %
442
59.81 %
63
8.53 %
63
8.53 %
221
29.91 %
PAR-RECUR
18
2,765
3.69 %
74
2.68 %
2464
89.11 %
191
6.91 %
154
5.57 %
73
2.64 %
Wiltshire
PAR-CUL-N
4
312
0.42 %
20
6.41 %
267
85.58 %
27
8.65 %
25
8.01 %
0
0.00 %
PAR-CUL-P
4
727
0.97 %
53
7.29 %
609
83.77 %
54
7.43 %
50
6.88 %
15
2.06 %
PAR-NEW-IN
1
19
0.03 %
1
5.26 %
13
68.42 %
5
26.32 %
5
26.32 %
0
0.00 %
PAR-OTHER
2
94
0.13 %
10
10.64 %
80
85.11 %
8
8.51 %
4
4.26 %
0
0.00 %
PAR-PERSIS
2
70
0.09 %
7
10.00 %
46
65.71 %
20
28.57 %
17
24.29 %
0
0.00 %
PAR-RECUR
3
431
0.58 %
32
7.42 %
360
83.53 %
40
9.28 %
37
8.58 %
2
0.46 %
Worcs
PAR-RECUR
2
33
0.04 %
6
18.18 %
24
72.73 %
3
9.09 %
3
9.09 %
0
0.00 %
England - Edge
Berks
PAR-RECUR
1
125
0.17 %
2
1.60 %
118
94.40 %
5
4.00 %
5
4.00 %
0
0.00 %
Bucks
PAR-CUL-N
1
224
0.30 %
10
4.46 %
204
91.07 %
11
4.91 %
10
4.46 %
0
0.00 %
PAR-NEW-IN
4
527
0.70 %
6
1.14 %
500
94.88 %
26
4.93 %
21
3.98 %
0
0.00 %
Cheshire
PAR-CUL-N
3
1,208
1.61 %
26
2.15 %
1122
92.88 %
72
5.96 %
60
4.97 %
0
0.00 %
PAR-CUL-P
2
85
0.11 %
0
0.00 %
56
65.88 %
29
34.12 %
29
34.12 %
0
0.00 %
PAR-NEW-IN
4
555
0.74 %
18
3.24 %
499
89.91 %
42
7.57 %
38
6.85 %
0
0.00 %
PAR-PERSIS
3
1,840
2.46 %
43
2.34 %
1709
92.88 %
131
7.12 %
85
4.62 %
3
0.16 %
PAR-RECUR
3
380
0.51 %
10
2.63 %
355
93.42 %
23
6.05 %
15
3.95 %
0
0.00 %
Derbyshire
PAR-NEW-IN
5
550
0.73 %
14
2.55 %
483
87.82 %
57
10.36 %
51
9.27 %
2
0.36 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
62
0.08 %
1
1.61 %
55
88.71 %
4
6.45 %
4
6.45 %
2
3.23 %
Hampshire
PAR-NEW-IN
3
454
0.61 %
16
3.52 %
405
89.21 %
33
7.27 %
30
6.61 %
3
0.66 %
Leics & Rut
PAR-CUL-N
1
30
0.04 %
3
10.00 %
26
86.67 %
2
6.67 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
9
1,374
1.84 %
35
2.55 %
1250
90.98 %
104
7.57 %
85
6.19 %
4
0.29 %
Northants
PAR-NEW-IN
8
983
1.31 %
21
2.14 %
841
85.55 %
129
13.12 %
121
12.31 %
0
0.00 %
PAR-RECUR
1
114
0.15 %
1
0.88 %
93
81.58 %
11
9.65 %
20
17.54 %
0
0.00 %
Notts
PAR-CUL-N
2
451
0.60 %
9
2.00 %
422
93.57 %
31
6.87 %
19
4.21 %
1
0.22 %
PAR-NEW-IN
4
447
0.60 %
11
2.46 %
410
91.72 %
33
7.38 %
26
5.82 %
0
0.00 %
Oxon
PAR-NEW-IN
11
1,587
2.12 %
90
5.67 %
1379
86.89 %
137
8.63 %
118
7.44 %
0
0.00 %
PAR-PERSIS
2
160
0.21 %
15
9.38 %
136
85.00 %
9
5.63 %
9
5.63 %
0
0.00 %
PAR-RECUR
2
147
0.20 %
3
2.04 %
131
89.12 %
15
10.20 %
13
8.84 %
0
0.00 %
Warks
PAR-RECUR
3
308
0.41 %
10
3.25 %
268
87.01 %
33
10.71 %
30
9.74 %
0
0.00 %
England - LRA
Beds
PAR-CUL-N
1
153
0.20 %
1
0.65 %
126
82.35 %
32
20.92 %
24
15.69 %
2
1.31 %
PAR-NEW-IN
2
94
0.13 %
1
1.06 %
89
94.68 %
3
3.19 %
3
3.19 %
1
1.06 %
Cambs
PAR-NEW-IN
1
72
0.10 %
1
1.39 %
66
91.67 %
4
5.56 %
4
5.56 %
1
1.39 %
Cumbria
PAR-NEW-IN
3
249
0.33 %
4
1.61 %
206
82.73 %
24
9.64 %
21
8.43 %
18
7.23 %
Gr Manchester
PAR-NEW-IN
5
245
0.33 %
1
0.41 %
230
93.88 %
18
7.35 %
14
5.71 %
0
0.00 %
Herts
PAR-NEW-IN
5
253
0.34 %
14
5.53 %
197
77.87 %
41
16.21 %
39
15.42 %
3
1.19 %
Kent
PAR-NEW-IN
1
63
0.08 %
2
3.17 %
56
88.89 %
5
7.94 %
5
7.94 %
0
0.00 %
Lancashire
SER-NSR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northumb
PAR-NEW-IN
1
248
0.33 %
10
4.03 %
208
83.87 %
19
7.66 %
17
6.85 %
13
5.24 %
N Yorks
PAR-NEW-IN
4
416
0.56 %
9
2.16 %
275
66.11 %
33
7.93 %
28
6.73 %
104
25.00 %
W Sussex
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
4
362
0.48 %
10
2.76 %
319
88.12 %
42
11.60 %
33
9.12 %
0
0.00 %
England
430
58596
78.26 %
2427
4.14 %
51455
87.81 %
4922
8.40 %
4202
7.17 %
512
0.87 %
Wales - High TB
Carms
PAR-IR-EX
3
11
0.01 %
4
36.36 %
7
63.64 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
16
97
0.13 %
39
40.21 %
39
40.21 %
8
8.25 %
6
6.19 %
13
13.40 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
19
61
0.08 %
15
24.59 %
37
60.66 %
3
4.92 %
3
4.92 %
6
9.84 %
PAR-RECUR
2
22
0.03 %
5
22.73 %
14
63.64 %
5
22.73 %
3
13.64 %
0
0.00 %
SER-FLEXI
11
1,669
2.23 %
106
6.35 %
1468
87.96 %
112
6.71 %
92
5.51 %
3
0.18 %
Ceredigion
PAR-IR-EX
7
22
0.03 %
5
22.73 %
16
72.73 %
0
0.00 %
0
0.00 %
1
4.55 %
PAR-IR-SEV
11
29
0.04 %
11
37.93 %
12
41.38 %
4
13.79 %
4
13.79 %
2
6.90 %
PAR-RAPID
5
9
0.01 %
3
33.33 %
3
33.33 %
1
11.11 %
1
11.11 %
2
22.22 %
PAR-RECUR
1
31
0.04 %
10
32.26 %
21
67.74 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
51
0.07 %
2
3.92 %
47
92.16 %
3
5.88 %
2
3.92 %
0
0.00 %
Gwent
PAR-IR-SEV
5
23
0.03 %
3
13.04 %
18
78.26 %
2
8.70 %
2
8.70 %
0
0.00 %
PAR-OTHER
4
144
0.19 %
2
1.39 %
129
89.58 %
18
12.50 %
13
9.03 %
0
0.00 %
PAR-RAPID
8
14
0.02 %
3
21.43 %
9
64.29 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-RECUR
3
70
0.09 %
0
0.00 %
66
94.29 %
6
8.57 %
3
4.29 %
1
1.43 %
SER-FLEXI
6
721
0.96 %
34
4.72 %
643
89.18 %
44
6.10 %
42
5.83 %
2
0.28 %
Pembs
PAR-IR-EX
14
68
0.09 %
24
35.29 %
43
63.24 %
2
2.94 %
1
1.47 %
0
0.00 %
PAR-IR-SEV
61
403
0.54 %
108
26.80 %
275
68.24 %
19
4.71 %
14
3.47 %
6
1.49 %
PAR-PERSIS
12
781
1.04 %
58
7.43 %
637
81.56 %
95
12.16 %
77
9.86 %
9
1.15 %
PAR-RAPID
56
143
0.19 %
43
30.07 %
90
62.94 %
10
6.99 %
9
6.29 %
1
0.70 %
PAR-RECUR
13
985
1.32 %
36
3.65 %
881
89.44 %
81
8.22 %
63
6.40 %
5
0.51 %
SER-FLEXI
30
2,578
3.44 %
259
10.05 %
2110
81.85 %
196
7.60 %
172
6.67 %
38
1.47 %
Powys
PAR-IR-EX
3
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
14
51
0.07 %
7
13.73 %
38
74.51 %
7
13.73 %
6
11.76 %
0
0.00 %
PAR-OTHER
5
163
0.22 %
9
5.52 %
137
84.05 %
18
11.04 %
17
10.43 %
0
0.00 %
PAR-PERSIS
2
258
0.34 %
1
0.39 %
243
94.19 %
16
6.20 %
14
5.43 %
0
0.00 %
PAR-POSTM
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
18
37
0.05 %
13
35.14 %
20
54.05 %
5
13.51 %
4
10.81 %
0
0.00 %
PAR-RECUR
5
253
0.34 %
15
5.93 %
219
86.56 %
22
8.70 %
19
7.51 %
0
0.00 %
S Wales
PAR-IR-SEV
1
4
0.01 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-IR-SEV
3
11
0.01 %
3
27.27 %
2
18.18 %
2
18.18 %
2
18.18 %
4
36.36 %
PAR-OTHER
1
2
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
2
100.00 %
PAR-RAPID
3
7
0.01 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
145
0.19 %
10
6.90 %
127
87.59 %
9
6.21 %
8
5.52 %
0
0.00 %
Ceredigion
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
5
0.01 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
6
21
0.03 %
4
19.05 %
17
80.95 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
12
54
0.07 %
10
18.52 %
44
81.48 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
19
2,279
3.04 %
39
1.71 %
2080
91.27 %
178
7.81 %
148
6.49 %
12
0.53 %
PAR-PERSIS
3
191
0.26 %
3
1.57 %
169
88.48 %
18
9.42 %
18
9.42 %
1
0.52 %
PAR-POSTM
23
87
0.12 %
8
9.20 %
75
86.21 %
5
5.75 %
4
4.60 %
0
0.00 %
PAR-RAPID
12
24
0.03 %
6
25.00 %
18
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
397
0.53 %
33
8.31 %
347
87.41 %
17
4.28 %
16
4.03 %
1
0.25 %
Powys
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
836
1.12 %
57
6.82 %
732
87.56 %
58
6.94 %
45
5.38 %
2
0.24 %
PAR-OTHER
2
214
0.29 %
8
3.74 %
182
85.05 %
28
13.08 %
24
11.21 %
0
0.00 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-OTHER
3
9
0.01 %
3
33.33 %
5
55.56 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-RAPID
2
9
0.01 %
7
77.78 %
2
22.22 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
115
0.15 %
26
22.61 %
72
62.61 %
21
18.26 %
17
14.78 %
0
0.00 %
Shropshire
PAR-IR-EX
2
5
0.01 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
2
113
0.15 %
3
2.65 %
101
89.38 %
9
7.96 %
9
7.96 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
29
1,788
2.39 %
74
4.14 %
1491
83.39 %
251
14.04 %
221
12.36 %
2
0.11 %
PAR-POSTM
16
50
0.07 %
4
8.00 %
45
90.00 %
1
2.00 %
1
2.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-HERD-S
2
40
0.05 %
4
10.00 %
28
70.00 %
8
20.00 %
8
20.00 %
0
0.00 %
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
5
238
0.32 %
6
2.52 %
215
90.34 %
20
8.40 %
15
6.30 %
2
0.84 %
PAR-POSTM
27
50
0.07 %
2
4.00 %
46
92.00 %
3
6.00 %
1
2.00 %
1
2.00 %
PAR-RAPID
2
9
0.01 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
2
384
0.51 %
38
9.90 %
312
81.25 %
40
10.42 %
34
8.85 %
0
0.00 %
Wales
545
15801
21.10 %
1174
7.43 %
13368
84.60 %
1351
8.55 %
1144
7.24 %
116
0.73 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wigtown
PAR-NEW-IN
2
472
0.63 %
12
2.54 %
435
92.16 %
32
6.78 %
25
5.30 %
0
0.00 %
Scotland
4
477
0.64 %
12
2.52 %
440
92.24 %
32
6.71 %
25
5.24 %
0
0.00 %
Grand Total
Sum:
979
74874
3613
65263
6305
5371
628
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol April 2022
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
90
9856
100.0%
391
4.0%
8799
89.3%
790
8.0%
655
6.6%
11
0.1%
Serial/Extended
1
1
0.0%
0
0.0 %
0
0.0 %
1 100.0%
1 100.0%
0
0.0 %
Total
91
9857
100.0%
391
4.0%
8799
89.3%
791
8.0%
656
6.7%
11
0.1%
Scotland
Parallel
2
472
100.0%
12
2.5%
435
92.2%
32
6.8%
25
5.3%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
472
100.0%
12
2.5%
435
92.2%
32
6.8%
25
5.3%
0
0.0 %
Wales
Parallel
112
2803
59.2%
166
5.9%
2439
87.0%
228
8.1%
193
6.9%
5
0.2%
Serial/Extended
15
1935
40.8%
118
6.1%
1706
88.2%
117
6.0%
104
5.4%
7
0.4%
Total
127
4738
100.0%
284
6.0%
4145
87.5%
345
7.3%
297
6.3%
12
0.3%
GB
Parallel
204
13131
87.2%
569
4.3%
11673
88.9%
1050
8.0%
873
6.6%
16
0.1%
Serial/Extended
16
1936
12.8%
118
6.1%
1706
88.1%
118
6.1%
105
5.4%
7
0.4%
Total
220
15067
100.0%
687
4.6%
13379
88.8%
1168
7.8%
978
6.5%
23
0.2%
91.00
127.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2022)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
425
58525
99.9%
2415
4.1%
51401
87.8%
4916
8.4%
4197
7.2%
512
0.9%
Serial/Extended
5
71
0.1%
12
16.9%
54
76.1%
6
8.5%
5
7.0%
0
0.0 %
Total
430
58596
100.0%
2427
4.1%
51455
87.8%
4922
8.4%
4202
7.2%
512
0.9%
Scotland
Parallel
2
472
99.0%
12
2.5%
435
92.2%
32
6.8%
25
5.3%
0
0.0 %
Serial/Extended
2
5
1.0%
0
0.0 %
5 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
4
477
100.0%
12
2.5%
440
92.2%
32
6.7%
25
5.2%
0
0.0 %
Wales
Parallel
485
9856
62.4%
692
7.0%
8314
84.4%
930
9.4%
778
7.9%
72
0.7%
Serial/Extended
60
5945
37.6%
482
8.1%
5054
85.0%
421
7.1%
366
6.2%
44
0.7%
Total
545
15801
100.0%
1174
7.4%
13368
84.6%
1351
8.6%
1144
7.2%
116
0.7%
GB
Parallel
912
68853
92.0%
3119
4.5%
60150
87.4%
5878
8.5%
5000
7.3%
584
0.8%
Serial/Extended
67
6021
8.0%
494
8.2%
5113
84.9%
427
7.1%
371
6.2%
44
0.7%
Total
979
74874
100.0%
3613
4.8%
65263
87.2%
6305
8.4%
5371
7.2%
628
0.8%
430.00
545.00
4.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
16
1936
12.8%
118
6.1%
1706
88.1%
118
6.1 %
105
5.4 %
7
0.4 %
Parallel – chronic breakdown
1
9
0.1%
0
0.0 %
5
55.6%
5
55.6 %
4
44.4 %
0
Parallel - IRs in Exit Strategy for persistent her
10
48
0.3%
14
29.2%
34
70.8%
0
0
0
Parallel New Infection
40
4700
31.2%
154
3.3%
4241
90.2%
357
7.6 %
295
6.3 %
10
0.2 %
Parallel - new infection cull area
7
863
5.7%
59
6.8%
755
87.5%
61
7.1 %
46
5.3 %
3
0.3 %
Parallel - Other
6
254
1.7%
9
3.5%
229
90.2%
26
10.2 %
16
6.3 %
0
Parallel Persistent Infection
13
1021
6.8%
38
3.7%
906
88.7%
95
9.3 %
77
7.5 %
0
Parallel – post-mvt test coupled with IDEXX an
15
56
0.4%
6
10.7%
50
89.3%
0
0
0
Parallel - Rapid Testing Two-times IRs
23
59
0.4%
16
27.1%
37
62.7%
6
10.2 %
6
10.2 %
0
Parallel - Recurrent breakdowns
55
5945
39.5%
231
3.9%
5289
89.0%
492
8.3 %
422
7.1 %
3
0.1 %
Parallel - severe IRs - in persistent herds
34
176
1.2%
42
23.9%
127
72.2%
8
4.5 %
7
4.0 %
0
Total
220
15067
100.0%
687
4.6%
13379
88.8%
1168
7.8 %
978
6.5 %
23
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (April 2022)
England - HRA
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel – chronic breakdown
1
9
0.1%
0
0.0 %
5
55.6%
5
55.6 %
4
44.4 %
0
Parallel New Infection
1
280
1.9%
21
7.5%
245
87.5%
21
7.5 %
14
5.0 %
0
Parallel - new infection cull area
4
382
2.5%
47
12.3%
307
80.4%
28
7.3 %
26
6.8 %
2
0.5 %
Parallel - Other
4
116
0.8%
8
6.9%
105
90.5%
9
7.8 %
3
2.6 %
0
Parallel Persistent Infection
7
619
4.1%
30
4.8%
544
87.9%
53
8.6 %
45
7.3 %
0
Parallel - Recurrent breakdowns
46
4739
31.5%
194
4.1%
4191
88.4%
410
8.7 %
353
7.4 %
1
0.0 %
England - HRA
64
6146
40.8%
300
4.9%
5397
87.8%
527
8.6 %
446
7.3 %
3
0.0 %
England - Edge
Parallel New Infection
11
2219
14.7%
53
2.4%
2032
91.6%
150
6.8 %
127
5.7 %
7
0.3 %
Parallel - new infection cull area
3
481
3.2%
12
2.5%
448
93.1%
33
6.9 %
20
4.2 %
1
0.2 %
Parallel - Recurrent breakdowns
7
713
4.7%
18
2.5%
649
91.0%
57
8.0 %
46
6.5 %
0
England - Edge
21
3413
22.7%
83
2.4%
3129
91.7%
240
7.0 %
193
5.7 %
8
0.2 %
England - LRA
Parallel New Infection
6
298
2.0%
8
2.7%
273
91.6%
24
8.1 %
17
5.7 %
0
England - LRA
6
298
2.0%
8
2.7%
273
91.6%
24
8.1 %
17
5.7 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
14
1930
12.8%
117
6.1%
1704
88.3%
115
6.0 %
102
5.3 %
7
0.4 %
Parallel - IRs in Exit Strategy for persistent her
8
35
0.2%
11
31.4%
24
68.6%
0
0
0
Parallel - Other
2
138
0.9%
1
0.7%
124
89.9%
17
12.3 %
13
9.4 %
0
Parallel Persistent Infection
6
402
2.7%
8
2.0%
362
90.0%
42
10.4 %
32
8.0 %
0
Parallel - Rapid Testing Two-times IRs
19
49
0.3%
15
30.6%
28
57.1%
6
12.2 %
6
12.2 %
0
Parallel - Recurrent breakdowns
2
493
3.3%
19
3.9%
449
91.1%
25
5.1 %
23
4.7 %
2
0.4 %
Parallel - severe IRs - in persistent herds
25
137
0.9%
34
24.8%
98
71.5%
6
4.4 %
5
3.6 %
0
Wales - High TB
76
3184
21.1%
205
6.4%
2789
87.6%
211
6.6 %
181
5.7 %
9
0.3 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
5
0.0%
1
20.0%
2
40.0%
2
40.0 %
2
40.0 %
0
Parallel - IRs in Exit Strategy for persistent her
2
13
0.1%
3
23.1%
10
76.9%
0
0
0
Parallel New Infection
11
655
4.3%
36
5.5%
561
85.6%
66
10.1 %
57
8.7 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX an
7
26
0.2%
2
7.7%
24
92.3%
0
0
0
Parallel - Rapid Testing Two-times IRs
4
10
0.1%
1
10.0%
9
90.0%
0
0
0
Parallel - severe IRs - in persistent herds
9
39
0.3%
8
20.5%
29
74.4%
2
5.1 %
2
5.1 %
0
Wales - Intermediate TB
34
748
5.0%
51
6.8%
635
84.9%
70
9.4 %
61
8.2 %
1
0.1 %
Wales - Low TB
Parallel New Infection
9
776
5.2%
24
3.1%
695
89.6%
64
8.2 %
55
7.1 %
2
0.3 %
Parallel – post-mvt test coupled with IDEXX an
8
30
0.2%
4
13.3%
26
86.7%
0
0
0
Wales - Low TB
17
806
5.3%
28
3.5%
721
89.5%
64
7.9 %
55
6.8 %
2
0.2 %
Scotland - Scotland
Parallel New Infection
2
472
3.1%
12
2.5%
435
92.2%
32
6.8 %
25
5.3 %
0
Scotland - Scotland
2
472
3.1%
12
2.5%
435
92.2%
32
6.8 %
25
5.3 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
64
6015
8.0%
494
8.2%
5107
84.9%
427
7.1 %
371
6.2 %
44
0.7 %
Parallel – chronic breakdown
4
710
0.9%
16
2.3%
647
91.1%
53
7.5 %
47
6.6 %
0
Parallel - Herd or Group Slaughter
2
40
0.1%
4
10.0%
28
70.0%
8
20.0 %
8
20.0 %
0
Parallel - IRs in Exit Strategy for persistent herds
37
134
0.2%
37
27.6%
93
69.4%
4
3.0 %
3
2.2 %
1
0.7 %
Parallel New Infection
144
14454
19.3%
474
3.3%
12680
87.7%
1319
9.1 %
1131
7.8 %
169
1.2 %
Parallel - new infection cull area
43
6791
9.1%
333
4.9%
5973
88.0%
554
8.2 %
474
7.0 %
11
0.2 %
Parallel - Other
34
xx-xxxx-xxxx
2.3%
85
5.0%
1496
88.2%
145
8.5 %
114
6.7 %
2
0.1 %
Parallel Persistent Infection
63
10073
13.5%
424
4.2%
8689
86.3%
881
8.7 %
723
7.2 %
237
2.4 %
Parallel - persistent infection cull area
21
3533
4.7%
165
4.7%
3067
86.8%
322
9.1 %
280
7.9 %
21
0.6 %
Parallel – post-mvt test coupled with IDEXX anti
68
190
0.3%
14
7.4%
169
88.9%
9
4.7 %
6
3.2 %
1
0.5 %
Parallel - Rapid Testing Two-times IRs
129
323
0.4%
97
30.0%
197
61.0%
22
6.8 %
20
6.2 %
9
2.8 %
Parallel - Recurrent breakdowns
243
30235
40.4%
1285
4.3%
26682
88.2%
2519
8.3 %
2160
7.1 %
108
0.4 %
Parallel - severe IRs - in persistent herds
124
673
0.9%
185
27.5%
429
63.7%
42
6.2 %
34
5.1 %
25
3.7 %
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
2
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Total
979
74874
100.0%
3613
4.8%
65263
87.2%
6305
8.4 %
5371
7.2 %
628
0.8 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2022)
England - HRA
Extended Flexible IFN-GAMMA test
4
70
0.1%
12
17.1%
53
75.7%
6
8.6 %
5
7.1 %
0
Parallel – chronic breakdown
4
710
0.9%
16
2.3%
647
91.1%
53
7.5 %
47
6.6 %
0
Parallel New Infection
2
299
0.4%
22
7.4%
258
86.3%
26
8.7 %
19
6.4 %
0
Parallel - new infection cull area
35
4725
6.3%
284
6.0%
4073
86.2%
406
8.6 %
360
7.6 %
8
0.2 %
Parallel - Other
18
1161
1.6%
63
5.4%
1039
89.5%
80
6.9 %
59
5.1 %
0
Parallel Persistent Infection
41
6843
9.1%
304
4.4%
5795
84.7%
612
8.9 %
520
7.6 %
224
3.3 %
Parallel - persistent infection cull area
19
3448
4.6%
165
4.8%
3011
87.3%
293
8.5 %
251
7.3 %
21
0.6 %
Parallel - Recurrent breakdowns
203
27570
36.8%
1164
4.2%
24341
88.3%
2288
8.3 %
1963
7.1 %
102
0.4 %
England - Edge
Parallel New Infection
50
6539
8.7%
212
3.2%
5822
89.0%
565
8.6 %
494
7.6 %
11
0.2 %
Parallel - new infection cull area
7
1913
2.6%
48
2.5%
1774
92.7%
116
6.1 %
90
4.7 %
1
0.1 %
Parallel Persistent Infection
5
2000
2.7%
58
2.9%
1845
92.3%
140
7.0 %
94
4.7 %
3
0.2 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
Parallel - persistent infection cull area
2
85
0.1%
0
0.0 %
56
65.9%
29
34.1 %
29
34.1 %
0
Parallel - Recurrent breakdowns
11
1076
1.4%
26
2.4%
967
89.9%
87
8.1 %
83
7.7 %
0
England - LRA
Parallel New Infection
27
2003
2.7%
52
2.6%
xx-xxxx-xxxx
82.2%
189
9.4 %
164
8.2 %
140
7.0 %
Parallel - new infection cull area
1
153
0.2%
1
0.7%
126
82.4%
32
20.9 %
24
15.7 %
2
1.3 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
England
430
58596
78.3%
2427
4.1%
51455
87.8%
4922
8.4 %
4202
7.2 %
512
0.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
49
5019
6.7%
401
8.0%
4268
85.0%
355
7.1 %
308
6.1 %
43
0.9 %
Parallel - IRs in Exit Strategy for persistent herd
27
106
0.1%
33
31.1%
71
67.0%
2
1.9 %
1
0.9 %
1
0.9 %
Parallel - Other
10
311
0.4%
11
3.5%
270
86.8%
36
11.6 %
30
9.6 %
0
Parallel Persistent Infection
14
1039
1.4%
59
5.7%
880
84.7%
111
10.7 %
91
8.8 %
9
0.9 %
Parallel – post-mvt test coupled with IDEXX ant
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
106
264
0.4%
77
29.2%
159
60.2%
21
8.0 %
19
7.2 %
9
3.4 %
Parallel - Recurrent breakdowns
24
1361
1.8%
66
4.8%
1201
88.2%
114
8.4 %
88
6.5 %
6
0.4 %
Parallel - severe IRs - in persistent herds
108
607
0.8%
172
28.3%
382
62.9%
40
6.6 %
32
5.3 %
21
3.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
9
542
0.7%
43
7.9%
474
87.5%
26
4.8 %
24
4.4 %
1
0.2 %
Parallel - IRs in Exit Strategy for persistent herd
9
27
0.0%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Parallel New Infection
29
3115
4.2%
96
3.1%
2812
90.3%
236
7.6 %
193
6.2 %
14
0.4 %
Parallel - Other
6
225
0.3%
11
4.9%
187
83.1%
29
12.9 %
25
11.1 %
2
0.9 %
Parallel Persistent Infection
3
191
0.3%
3
1.6%
169
88.5%
18
9.4 %
18
9.4 %
1
0.5 %
Parallel – post-mvt test coupled with IDEXX ant
24
88
0.1%
8
9.1%
76
86.4%
5
5.7 %
4
4.5 %
0
Parallel - Rapid Testing Two-times IRs
20
49
0.1%
19
38.8%
30
61.2%
0
0
0
Parallel - Recurrent breakdowns
5
228
0.3%
29
12.7%
173
75.9%
30
13.2 %
26
11.4 %
0
Parallel - severe IRs - in persistent herds
16
66
0.1%
13
19.7%
47
71.2%
2
3.0 %
2
3.0 %
4
6.1 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
384
0.5%
38
9.9%
312
81.3%
40
10.4 %
34
8.9 %
0
Parallel - Herd or Group Slaughter
2
40
0.1%
4
10.0%
28
70.0%
8
20.0 %
8
20.0 %
0
Parallel - IRs in Exit Strategy for persistent herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
34
2026
2.7%
80
3.9%
1706
84.2%
271
13.4 %
236
11.6 %
4
0.2 %
Parallel – post-mvt test coupled with IDEXX ant
43
100
0.1%
6
6.0%
91
91.0%
4
4.0 %
2
2.0 %
1
1.0 %
Parallel - Rapid Testing Two-times IRs
3
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Wales
545
15801
21.1%
1174
7.4%
13368
84.6%
1351
8.6 %
1144
7.2 %
116
0.7 %
Scotland - Scotland
Parallel New Infection
2
472
0.6%
12
2.5%
435
92.2%
32
6.8 %
25
5.3 %
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Scotland
4
477
0.6%
12
2.5%
440
92.2%
32
6.7 %
25
5.2 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2021 May
357
27182
214
1564
3
65
0
0
0
0
130
4
0
9
0
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
4
0
4
0
1083
0
92
0
6
July
298
24841
164
1077
30
0
0
0
0
61
4
0
4
0
936
0
40
0
2
August
312
17920
167
912
3
26
0
0
0
0
35
2
0
7
0
755
0
81
1
2
September
274
15805
130
890
13
0
0
0
0
95
1
0
7
0
742
0
31
0
0
October
276
19790
140
814
15
0
0
0
0
44
3
2
17
0
696
0
36
1
0
November
329
25737
195
1349
26
0
0
0
0
92
1
0
25
1
1143
0
61
0
0
December
238
13117
138
759
14
0
0
0
0
42
5
2
1
0
656
0
36
0
2
2022 January
262
18563
129
852
19
0
0
0
0
32
1
0
17
0
745
1
36
0
0
February
229
18198
132
966
1
20
0
0
0
0
84
0
3
3
0
764
53
38
0
0
March
268
23046
165
1108
97
0
0
0
0
59
0
13
5
0
883
50
0
0
1
April
220
15067
130
687
137
379
0
0
0
0
11
0
0
0
0
34
5
0
0
0
Totals (last
12 months)
3418
247094
1912
12292
145
723
0
0
0
0
790
25
20
99
1
9657
109
582
3
14
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1599
192714
1041
8728
512
0
0
0
0
730
14
9
46
1
7247
5
39
0
0
Scotland
18
1761
12
66
0
0
0
0
0
16
0
0
0
0
23
0
10
0
5
Wales
1801
52619
859
3498
211
0
0
0
0
44
11
11
53
0
2387
104
533
3
9
Totals
3418
247094
1912
12292
723
0
0
0
0
790
25
20
99
1
9657
109
582
3
14
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2020
2021
2022
IFNg Positive herds
IFNg Negative herds
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2020
2021
2022
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2020
2021
2022
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
W Yorks
Wiltshire
Warks
Somerset excl N
Powys
Oxon
Notts
Leics & Rut
Heref
Gwynedd
Gr Manchester
Dorset
Derbyshire
Cheshire
Carms
Beds
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during April 2022 (% submission per County)
County
%Resampled %Retested
Avon
7.71 %
9.63 %
Beds
Bucks
3.57 %
4.56 %
Carms
3.69 %
5.37 %
Ceredigion
Cheshire
4.24 %
6.12 %
Corn & Scilly
3.14 %
3.92 %
Derbyshire
Devon
10.29 %
10.96 %
Dorset
5.10 %
7.82 %
Gloucs
7.28 %
8.47 %
Gr Manchester
5.56 %
8.33 %
Gwent
6.44 %
7.12 %
Gwynedd
7.08 %
8.36 %
Hampshire
6.70 %
7.37 %
Heref
5.70 %
7.28 %
Kent
7.94 %
7.94 %
Leics & Rut
6.12 %
7.44 %
NE Wales
7.89 %
8.75 %
Notts
4.72 %
6.96 %
N Yorks
25.00 %
25.00 %
Oxon
6.96 %
7.59 %
Pembs
6.01 %
7.12 %
Powys
5.62 %
6.40 %
Shropshire
5.28 %
6.92 %
Somerset excl
4.50 %
5.58 %
Staffs
28.57 %
30.16 %
Warks
9.74 %
10.71 %
Wigtown
5.30 %
6.78 %
Wiltshire
50.00 %
Worcs
9.09 %
9.09 %
W Yorks
3.95 %
7.24 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
9730
391
4.0%
9857
391
4.0%
Wales
4634
284
6.1%
4738
284
6.0%
Scotland
472
12
2.5%
472
12
2.5%
GB
14836
687
4.6%
15067
687
4.6%
Table 6a (i): Number of animals and submissions tested in April 2022
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
56151
2427
4.3%
58596
2427
4.1%
Wales
15019
1174
7.8%
15801
1174
7.4%
Scotland
477
12
2.5%
477
12
2.5%
GB
71647
3613
5.0%
74874
3613
4.8%
Table 6b (i): Number of animals and submissions tested in 2022 Up to end of April
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20826
1098
5.3%
21172
1098
5.2%
Wales
6510
412
6.3%
6622
412
6.2%
Scotland
314
61
19.4%
314
61
19.4%
GB
27650
1571
5.7%
28108
1571
5.6%
Table 6a (ii): Number of animals and submissions tested in April 2021
Table 6b (ii): Number of animals and submissions tested in 2021 Up to end of April
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
89490
5037
5.6%
93451
5037
5.4%
Wales
25761
1595
6.2%
26961
1595
5.9%
Scotland
394
96
24.4%
739
96
13.0%
GB
115639
6728
5.8%
121151
6728
5.6%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Herefordshire
PRV-SP
1
1
0
0
Leicestershire and Rutland
PRV-SP
1
1
0
0
Somerset excl North
PRV-SP
2
13
1
7.69 %
Total
4
15
1
6.67 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples April 2022
Table 7b Number of private samples 2022
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Hampshire
PRV-SE
1
7
2
28.57 %
Herefordshire
PRV-SP
1
1
0
0
Leicestershire and Rutland
PRV-SP
1
1
0
0
Somerset excl North
PRV-SP
3
17
1
5.88 %
Wiltshire
PRV-SE
1
1
0
0
Total
8
29
3
10.34 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Not Known
BOV-IR-C
1
8
2
25.0%
Not Known
1
8
2
25.0%
Wales
Carmarthenshire
BOV-IR-C
5
30
6
20.0%
BOV-OTHER
1
4
0
0
Ceredigion
BOV-IR-C
1
1
0
0
North East Wales
BOV-IR-C
7
22
4
18.2%
BOV-IR-EX
1
11
3
27.3%
BOV-POSTM
14
49
7
14.3%
North West Wales
BOV-POSTM
1
1
0
0
Pembrokeshire
BOV-IR-C
22
175
54
30.9%
BOV-IR-EX
3
15
4
26.7%
BOV-OTHER
1
64
17
26.6%
BOV-P
3
82
2
2.4%
OTHER
1
30
0
0
Powys
BOV-IR-C
4
22
9
40.9%
BOV-P
1
23
0
0
South Wales
BOV-IR-C
3
20
2
10.0%
BOV-OTHER
1
25
4
16.0%
Wales
69
574
112
19.5%
All GB
70
582
114
19.6 %
Table 8a Number of Cattle Antibody Tests April 2022
Table 8b Number of Cattle Antibody Tests 2022
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Not Known
BOV-IR-C
1
8
2
25.0%
Not Known
1
8
2
25.0%
Wales
Carmarthenshire
BOV-IR-C
11
102
32
31.4%
BOV-IR-EX
1
10
1
10.0%
BOV-OTHER
2
156
7
4.5%
Ceredigion
BOV-IR-C
9
74
8
10.8%
BOV-IR-EX
3
11
3
27.3%
North East Wales
BOV-IR-C
16
120
19
15.8%
BOV-IR-EX
2
14
4
28.6%
BOV-OTHER
1
17
0
0
BOV-P
1
420
43
10.2%
BOV-POSTM
37
96
20
20.8%
North West Wales
BOV-POSTM
2
2
0
0
Pembrokeshire
BOV-IR-C
56
488
122
25.0%
BOV-IR-EX
10
46
14
30.4%
BOV-OTHER
4
240
23
9.6%
BOV-P
5
291
20
6.9%
OTHER
1
30
0
0
Powys
BOV-IR-C
10
60
13
21.7%
BOV-P
1
23
0
0
BOV-POSTM
1
1
0
0

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
South Wales
BOV-IR-C
5
28
3
10.7%
BOV-OTHER
2
30
5
16.7%
Wales
180
2,259
337
14.9%
All GB
181
2,267
339
15.0 %