Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - April 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 164
Report Period 1st - 30th April 2020
Issued 19 May 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
April 2020
%
Total 2020
%
Submissions
184
1112
Samples
Total (%)
10928
100.00 %
93569
100.00 %
England
7482
68.47 %
73317
78.36 %
Wales
2984
27.31 %
17590
18.80 %
Scotland
462
4.23 %
2662
2.84 %
Parallel Tests
PAR-CUL-N
1461
13.37 %
29044
31.04 %
PAR-CUL-P
917
8.39 %
4832
5.16 %
PAR-HERD-S
28
0.26 %
307
0.33 %
PAR-NEW-IN
7338
67.15 %
42815
45.76 %
PAR-OTHER
133
1.22 %
1755
1.88 %
PAR-PERSIS
898
8.22 %
12507
13.37 %
PAR-RAPID
142
1.30 %
441
0.47 %
Total(% of all samples)
10917
99.90 %
91701
98.00 %
Serial/Extended Tests
SER-FLEXI
11
0.10 %
1868
2.00 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
Total(% of all samples)
11
0.10 %
1868
2.00 %
Total
10928
100.00 %
93569
100.00 %
Retests
Total (% of all samples)
687
6.29 %
5930
6.34 %
England
482
4.41 %
4495
4.80 %
Wales
187
1.71 %
1288
1.38 %
Scotland
18
0.16 %
147
0.16 %
Resamples
Total (% of all samples)
535
4.90 %
4678
5.00 %
England
379
3.47 %
3524
3.77 %
Wales
142
1.30 %
1028
1.10 %
Scotland
14
0.13 %
126
0.13 %
Rejects
Total (% of all samples)
3
0.03 %
1758
1.88 %
England
0
0.00 %
1668
1.78 %
Wales
3
0.03 %
25
0.03 %
Scotland
0
0.00 %
65
0.07 %
Table 1. Monthly Totals for April 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
1461
13.4 %
PAR-CUL-P
917
8.4 %
PAR-HERD-S
28
0.3 %
PAR-NEW-IN
7338
67.1 %
PAR-OTHER
133
1.2 %
PAR-PERSIS
898
8.2 %
PAR-RAPID
142
1.3 %
SER-FLEXI
11
0.1 %
Total
10928
100.00 %
Figure 1. Number of samples submitted in each category during April 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
200
400
600
800
1000
1200
1400
1600
Wigtown
Kirkcudbright
Argyll & Bute
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Warks
Somerset excl N
Shropshire
Oxon
N Yorks
Northants
Leics & Rut
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during April 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
2000
4000
6000
8000
10000
12000
Wigtown
Kirkcudbright
Dumfries & G
Berwickshire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
5
240
2.20 %
10
4.17 %
222
92.50 %
11
4.58 %
8
3.33 %
0
0.00 %
PAR-CUL-P
2
638
5.84 %
7
1.10 %
625
97.96 %
13
2.04 %
6
0.94 %
0
0.00 %
PAR-PERSIS
1
84
0.77 %
1
1.19 %
74
88.10 %
10
11.90 %
9
10.71 %
0
0.00 %
Devon
PAR-CUL-N
9
626
5.73 %
22
3.51 %
573
91.53 %
38
6.07 %
31
4.95 %
0
0.00 %
Dorset
PAR-CUL-N
2
168
1.54 %
8
4.76 %
151
89.88 %
13
7.74 %
9
5.36 %
0
0.00 %
PAR-CUL-P
1
279
2.55 %
25
8.96 %
235
84.23 %
22
7.89 %
19
6.81 %
0
0.00 %
PAR-PERSIS
1
532
4.87 %
41
7.71 %
449
84.40 %
54
10.15 %
42
7.89 %
0
0.00 %
Gloucs
PAR-CUL-N
3
404
3.70 %
22
5.45 %
362
89.60 %
22
5.45 %
20
4.95 %
0
0.00 %
Shropshire
PAR-CUL-N
1
3
0.03 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Somerset excl N
PAR-CUL-N
4
20
0.18 %
2
10.00 %
17
85.00 %
1
5.00 %
1
5.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
827
7.57 %
22
2.66 %
749
90.57 %
73
8.83 %
56
6.77 %
0
0.00 %
Bucks
PAR-NEW-IN
1
24
0.22 %
0
0.00 %
22
91.67 %
3
12.50 %
2
8.33 %
0
0.00 %
Cheshire
PAR-NEW-IN
9
1,157
10.59 %
25
2.16 %
1074
92.83 %
72
6.22 %
58
5.01 %
0
0.00 %
Derbyshire
PAR-NEW-IN
7
941
8.61 %
31
3.29 %
874
92.88 %
39
4.14 %
36
3.83 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
435
3.98 %
12
2.76 %
401
92.18 %
24
5.52 %
22
5.06 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
218
1.99 %
4
1.83 %
206
94.50 %
19
8.72 %
8
3.67 %
0
0.00 %
Northants
PAR-NEW-IN
2
36
0.33 %
1
2.78 %
34
94.44 %
2
5.56 %
1
2.78 %
0
0.00 %
Oxon
PAR-NEW-IN
2
48
0.44 %
3
6.25 %
41
85.42 %
5
10.42 %
4
8.33 %
0
0.00 %
Warks
PAR-NEW-IN
5
260
2.38 %
13
5.00 %
229
88.08 %
23
8.85 %
18
6.92 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
2
0.02 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
4
540
4.94 %
5
0.93 %
506
93.70 %
38
7.04 %
29
5.37 %
0
0.00 %
England
67
7482
68.47 %
254
3.39 %
6849
91.54 %
482
6.44 %
379
5.07 %
0
0.00 %
Wales - High TB
Carms
PAR-HERD-S
2
27
0.25 %
2
7.41 %
24
88.89 %
1
3.70 %
1
3.70 %
0
0.00 %
PAR-OTHER
3
23
0.21 %
3
13.04 %
18
78.26 %
3
13.04 %
2
8.70 %
0
0.00 %
PAR-PERSIS
3
81
0.74 %
5
6.17 %
72
88.89 %
4
4.94 %
4
4.94 %
0
0.00 %
PAR-RAPID
9
33
0.30 %
5
15.15 %
26
78.79 %
3
9.09 %
2
6.06 %
0
0.00 %
Table 2a. Summary by County for April 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-OTHER
3
5
0.05 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-PERSIS
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-RAPID
6
25
0.23 %
6
24.00 %
16
64.00 %
3
12.00 %
3
12.00 %
0
0.00 %
Gwent
PAR-RAPID
1
5
0.05 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
12
83
0.76 %
17
20.48 %
63
75.90 %
3
3.61 %
3
3.61 %
0
0.00 %
PAR-PERSIS
7
156
1.43 %
8
5.13 %
137
87.82 %
12
7.69 %
11
7.05 %
0
0.00 %
PAR-RAPID
16
52
0.48 %
13
25.00 %
33
63.46 %
6
11.54 %
6
11.54 %
0
0.00 %
Powys
PAR-HERD-S
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
44
0.40 %
3
6.82 %
40
90.91 %
1
2.27 %
1
2.27 %
0
0.00 %
PAR-RAPID
6
9
0.08 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
S Wales
PAR-RAPID
1
2
0.02 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
3
17
0.16 %
3
17.65 %
13
76.47 %
1
5.88 %
1
5.88 %
0
0.00 %
NE Wales
PAR-NEW-IN
12
902
8.25 %
55
6.10 %
795
88.14 %
67
7.43 %
52
5.76 %
0
0.00 %
PAR-RAPID
2
3
0.03 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
10
0.09 %
1
10.00 %
9
90.00 %
1
10.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
1
183
1.67 %
5
2.73 %
170
92.90 %
7
3.83 %
7
3.83 %
1
0.55 %
S Glamorgan
PAR-RAPID
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
8
765
7.00 %
9
1.18 %
730
95.42 %
40
5.23 %
24
3.14 %
2
0.26 %
PAR-RAPID
1
11
0.10 %
0
0.00 %
8
72.73 %
3
27.27 %
3
27.27 %
0
0.00 %
NE Wales
PAR-NEW-IN
5
538
4.92 %
6
1.12 %
514
95.54 %
28
5.20 %
18
3.35 %
0
0.00 %
PAR-OTHER
1
5
0.05 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
112
2984
27.31 %
145
4.86 %
2694
90.28 %
187
6.27 %
142
4.76 %
3
0.10 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
16
0.15 %
1
6.25 %
9
56.25 %
6
37.50 %
6
37.50 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
2
85
0.78 %
5
5.88 %
78
91.76 %
3
3.53 %
2
2.35 %
0
0.00 %
Wigtown
PAR-NEW-IN
2
361
3.30 %
3
0.83 %
352
97.51 %
9
2.49 %
6
1.66 %
0
0.00 %
Scotland
5
462
4.23 %
9
1.95 %
439
95.02 %
18
3.90 %
14
3.03 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Grand Total
Sum:
184
10928
408
9982
687
535
3
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
33
4,414
4.72 %
143
3.24 %
4087
92.59 %
246
5.57 %
184
4.17 %
0
0.00 %
PAR-CUL-P
6
1,796
1.92 %
45
2.51 %
xx-xxxx-xxxx
94.43 %
71
3.95 %
55
3.06 %
0
0.00 %
PAR-PERSIS
17
3,332
3.56 %
135
4.05 %
3079
92.41 %
171
5.13 %
117
3.51 %
1
0.03 %
Devon
PAR-CUL-N
98
10,914
11.66 %
433
3.97 %
9831
90.08 %
675
6.18 %
546
5.00 %
104
0.95 %
PAR-CUL-P
7
726
0.78 %
34
4.68 %
633
87.19 %
72
9.92 %
59
8.13 %
0
0.00 %
PAR-PERSIS
1
92
0.10 %
8
8.70 %
80
86.96 %
8
8.70 %
4
4.35 %
0
0.00 %
SER-FLEXI
3
50
0.05 %
2
4.00 %
46
92.00 %
2
4.00 %
2
4.00 %
0
0.00 %
Dorset
PAR-CUL-N
20
2,440
2.61 %
160
6.56 %
2023
82.91 %
132
5.41 %
106
4.34 %
151
6.19 %
PAR-CUL-P
6
1,440
1.54 %
162
11.25 %
1225
85.07 %
80
5.56 %
52
3.61 %
1
0.07 %
PAR-NEW-IN
2
167
0.18 %
5
2.99 %
156
93.41 %
11
6.59 %
6
3.59 %
0
0.00 %
PAR-PERSIS
1
532
0.57 %
41
7.71 %
449
84.40 %
54
10.15 %
42
7.89 %
0
0.00 %
SER-FLEXI
5
740
0.79 %
48
6.49 %
674
91.08 %
30
4.05 %
18
2.43 %
0
0.00 %
Gloucs
PAR-CUL-N
25
2,409
2.57 %
125
5.19 %
2127
88.29 %
189
7.85 %
157
6.52 %
0
0.00 %
Heref
PAR-CUL-N
9
877
0.94 %
33
3.76 %
812
92.59 %
43
4.90 %
29
3.31 %
3
0.34 %
PAR-NEW-IN
3
248
0.27 %
10
4.03 %
207
83.47 %
38
15.32 %
30
12.10 %
1
0.40 %
PAR-PERSIS
2
213
0.23 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-CUL-N
3
416
0.44 %
14
3.37 %
385
92.55 %
21
5.05 %
15
3.61 %
2
0.48 %
PAR-PERSIS
7
1,015
1.08 %
60
5.91 %
899
88.57 %
65
6.40 %
54
5.32 %
2
0.20 %
SER-FLEXI
3
389
0.42 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
41
3,227
3.45 %
119
3.69 %
2972
92.10 %
152
4.71 %
108
3.35 %
28
0.87 %
PAR-CUL-P
1
261
0.28 %
13
4.98 %
238
91.19 %
13
4.98 %
10
3.83 %
0
0.00 %
PAR-PERSIS
2
286
0.31 %
25
8.74 %
253
88.46 %
11
3.85 %
8
2.80 %
0
0.00 %
Staffs
PAR-CUL-N
6
968
1.03 %
37
3.82 %
729
75.31 %
51
5.27 %
47
4.86 %
155
16.01 %
Wiltshire
PAR-CUL-N
26
2,965
3.17 %
100
3.37 %
2679
90.35 %
226
7.62 %
172
5.80 %
14
0.47 %
PAR-CUL-P
4
609
0.65 %
37
6.08 %
547
89.82 %
38
6.24 %
25
4.11 %
0
0.00 %
PAR-PERSIS
2
104
0.11 %
5
4.81 %
93
89.42 %
7
6.73 %
6
5.77 %
0
0.00 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
2
45
0.05 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
14
1,716
1.83 %
37
2.16 %
1563
91.08 %
151
8.80 %
116
6.76 %
0
0.00 %
Bucks
PAR-NEW-IN
9
970
1.04 %
53
5.46 %
836
86.19 %
98
10.10 %
81
8.35 %
0
0.00 %
Cheshire
PAR-CUL-N
2
51
0.05 %
1
1.96 %
42
82.35 %
10
19.61 %
8
15.69 %
0
0.00 %
PAR-NEW-IN
68
11,036
11.79 %
247
2.24 %
10268
93.04 %
582
5.27 %
471
4.27 %
50
0.45 %
Derbyshire
PAR-NEW-IN
54
6,745
7.21 %
223
3.31 %
5673
84.11 %
486
7.21 %
417
6.18 %
432
6.40 %
E Sussex
PAR-NEW-IN
11
1,113
1.19 %
42
3.77 %
1031
92.63 %
48
4.31 %
40
3.59 %
0
0.00 %
Hampshire
PAR-CUL-N
2
363
0.39 %
15
4.13 %
327
90.08 %
39
10.74 %
19
5.23 %
2
0.55 %
PAR-NEW-IN
8
855
0.91 %
21
2.46 %
787
92.05 %
52
6.08 %
47
5.50 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
14
1,228
1.31 %
39
3.18 %
1124
91.53 %
77
6.27 %
49
3.99 %
16
1.30 %
Northants
PAR-NEW-IN
7
334
0.36 %
9
2.69 %
306
91.62 %
24
7.19 %
19
5.69 %
0
0.00 %
Notts
PAR-NEW-IN
1
75
0.08 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Oxon
PAR-NEW-IN
37
3,489
3.73 %
142
4.07 %
2896
83.00 %
222
6.36 %
163
4.67 %
288
8.25 %
Warks
PAR-NEW-IN
28
2,882
3.08 %
75
2.60 %
2582
89.59 %
157
5.45 %
121
4.20 %
104
3.61 %
England - LRA
Cumbria
PAR-NEW-IN
1
30
0.03 %
0
0.00 %
29
96.67 %
2
6.67 %
1
3.33 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
5
268
0.29 %
1
0.37 %
54
20.15 %
3
1.12 %
3
1.12 %
210
78.36 %
Humberside
PAR-NEW-IN
2
78
0.08 %
7
8.97 %
66
84.62 %
7
8.97 %
5
6.41 %
0
0.00 %
Lincs
PAR-NEW-IN
3
176
0.19 %
7
3.98 %
162
92.05 %
7
3.98 %
7
3.98 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
49
0.05 %
1
2.04 %
29
59.18 %
6
12.24 %
6
12.24 %
13
26.53 %
N Yorks
PAR-NEW-IN
7
755
0.81 %
7
0.93 %
628
83.18 %
39
5.17 %
29
3.84 %
91
12.05 %
Surrey
PAR-NEW-IN
3
429
0.46 %
17
3.96 %
396
92.31 %
18
4.20 %
16
3.73 %
0
0.00 %
England
615
73317
78.36 %
2769
3.78 %
65356
89.14 %
4495
6.13 %
3524
4.81 %
1668
2.28 %
Wales - High TB
Carms
PAR-HERD-S
6
234
0.25 %
19
8.12 %
207
88.46 %
15
6.41 %
8
3.42 %
0
0.00 %
PAR-NEW-IN
1
8
0.01 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
14
234
0.25 %
31
13.25 %
186
79.49 %
19
8.12 %
17
7.26 %
0
0.00 %
PAR-PERSIS
24
1,547
1.65 %
109
7.05 %
1385
89.53 %
73
4.72 %
51
3.30 %
2
0.13 %
PAR-RAPID
28
119
0.13 %
21
17.65 %
89
74.79 %
13
10.92 %
9
7.56 %
0
0.00 %
SER-FLEXI
2
27
0.03 %
7
25.93 %
20
74.07 %
1
3.70 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-HERD-S
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
8
26
0.03 %
0
0.00 %
23
88.46 %
3
11.54 %
3
11.54 %
0
0.00 %
PAR-PERSIS
16
429
0.46 %
19
4.43 %
390
90.91 %
22
5.13 %
18
4.20 %
2
0.47 %
PAR-RAPID
13
56
0.06 %
17
30.36 %
35
62.50 %
4
7.14 %
4
7.14 %
0
0.00 %
Gwent
PAR-HERD-S
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
13
541
0.58 %
29
5.36 %
495
91.50 %
32
5.91 %
17
3.14 %
0
0.00 %
PAR-RAPID
10
22
0.02 %
6
27.27 %
16
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
133
0.14 %
17
12.78 %
113
84.96 %
7
5.26 %
3
2.26 %
0
0.00 %
Pembs
PAR-HERD-S
2
5
0.01 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-OTHER
36
694
0.74 %
82
11.82 %
581
83.72 %
40
5.76 %
31
4.47 %
0
0.00 %
PAR-PERSIS
54
2,565
2.74 %
166
6.47 %
2221
86.59 %
203
7.91 %
171
6.67 %
7
0.27 %
PAR-RAPID
39
160
0.17 %
36
22.50 %
116
72.50 %
7
4.38 %
8
5.00 %
0
0.00 %
SER-FLEXI
3
37
0.04 %
0
0.00 %
35
94.59 %
4
10.81 %
2
5.41 %
0
0.00 %
Powys
PAR-HERD-S
8
31
0.03 %
3
9.68 %
28
90.32 %
2
6.45 %
0
0.00 %
0
0.00 %
PAR-OTHER
9
747
0.80 %
34
4.55 %
639
85.54 %
82
10.98 %
74
9.91 %
0
0.00 %
PAR-PERSIS
28
1,046
1.12 %
60
5.74 %
848
81.07 %
145
13.86 %
130
12.43 %
8
0.76 %
PAR-RAPID
13
38
0.04 %
5
13.16 %
32
84.21 %
1
2.63 %
1
2.63 %
0
0.00 %
SER-FLEXI
10
212
0.23 %
17
8.02 %
186
87.74 %
19
8.96 %
9
4.25 %
0
0.00 %
S Wales
PAR-PERSIS
1
69
0.07 %
3
4.35 %
64
92.75 %
6
8.70 %
2
2.90 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
36
0.04 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-PERSIS
2
14
0.01 %
0
0.00 %
12
85.71 %
3
21.43 %
2
14.29 %
0
0.00 %
SER-FLEXI
1
9
0.01 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
30
0.03 %
5
16.67 %
24
80.00 %
3
10.00 %
1
3.33 %
0
0.00 %
PAR-OTHER
4
18
0.02 %
3
16.67 %
14
77.78 %
1
5.56 %
1
5.56 %
0
0.00 %
PAR-PERSIS
3
198
0.21 %
23
11.62 %
167
84.34 %
8
4.04 %
8
4.04 %
0
0.00 %
PAR-RAPID
3
3
0.00 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-PERSIS
2
7
0.01 %
4
57.14 %
2
28.57 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
48
0.05 %
6
12.50 %
40
83.33 %
2
4.17 %
2
4.17 %
0
0.00 %
NE Wales
PAR-NEW-IN
36
4,085
4.37 %
130
3.18 %
3687
90.26 %
306
7.49 %
268
6.56 %
0
0.00 %
PAR-OTHER
1
15
0.02 %
2
13.33 %
13
86.67 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-PERSIS
10
477
0.51 %
61
12.79 %
401
84.07 %
20
4.19 %
15
3.14 %
0
0.00 %
PAR-RAPID
7
26
0.03 %
3
11.54 %
21
80.77 %
2
7.69 %
2
7.69 %
0
0.00 %
SER-FLEXI
7
129
0.14 %
6
4.65 %
114
88.37 %
12
9.30 %
7
5.43 %
2
1.55 %
Powys
PAR-NEW-IN
7
764
0.82 %
13
1.70 %
715
93.59 %
45
5.89 %
35
4.58 %
1
0.13 %
PAR-OTHER
1
12
0.01 %
0
0.00 %
10
83.33 %
3
25.00 %
2
16.67 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
13
0.01 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
15
1,075
1.15 %
13
1.21 %
1018
94.70 %
63
5.86 %
41
3.81 %
3
0.28 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-RAPID
2
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
10
1,578
1.69 %
19
1.20 %
1490
94.42 %
102
6.46 %
69
4.37 %
0
0.00 %
PAR-OTHER
1
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
40
0.04 %
0
0.00 %
32
80.00 %
10
25.00 %
8
20.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
471
17590
18.80 %
984
5.59 %
15553
88.42 %
1288
7.32 %
1028
5.84 %
25
0.14 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
7
588
0.63 %
32
5.44 %
455
77.38 %
41
6.97 %
36
6.12 %
65
11.05 %
Ayrshire
PAR-NEW-IN
3
383
0.41 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Berwickshire
PAR-NEW-IN
2
78
0.08 %
8
10.26 %
62
79.49 %
8
10.26 %
8
10.26 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
6
849
0.91 %
12
1.41 %
802
94.46 %
38
4.48 %
35
4.12 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
3
106
0.11 %
5
4.72 %
99
93.40 %
3
2.83 %
2
1.89 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
658
0.70 %
17
2.58 %
619
94.07 %
27
4.10 %
22
3.34 %
0
0.00 %
Scotland
26
2662
2.84 %
75
2.82 %
2396
90.01 %
147
5.52 %
126
4.73 %
65
2.44 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Grand Total
Sum:
1112
93569
3828
83305
5930
4678
1758
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol April 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
67
7482
100.0%
254
3.4%
6849
91.5%
482
6.4%
379
5.1%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
67
7482
100.0%
254
3.4%
6849
91.5%
482
6.4%
379
5.1%
0
0.0 %
Scotland
Parallel
5
462
100.0%
9
1.9%
439
95.0%
18
3.9%
14
3.0%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
5
462
100.0%
9
1.9%
439
95.0%
18
3.9%
14
3.0%
0
0.0 %
Wales
Parallel
109
2973
99.6%
144
4.8%
2685
90.3%
185
6.2%
141
4.7%
3
0.1%
Serial/Extended
3
11
0.4%
1
9.1%
9
81.8%
2
18.2%
1
9.1%
0
0.0 %
Total
112
2984
100.0%
145
4.9%
2694
90.3%
187
6.3%
142
4.8%
3
0.1%
GB
Parallel
181
10917
99.9%
407
3.7%
9973
91.4%
685
6.3%
534
4.9%
3
0.0%
Serial/Extended
3
11
0.1%
1
9.1%
9
81.8%
2
18.2%
1
9.1%
0
0.0 %
Total
184
10928
100.0%
408
3.7%
9982
91.3%
687
6.3%
535
4.9%
3
0.0%
67.00
112.00
5.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
602
72093
98.3%
2699
3.7%
64243
89.1%
4436
6.2%
3483
4.8%
1668
2.3%
Serial/Extended
13
1224
1.7%
70
5.7%
1113
90.9%
59
4.8%
41
3.3%
0
0.0 %
Total
615
73317
100.0%
2769
3.8%
65356
89.1%
4495
6.1%
3524
4.8%
1668
2.3%
Scotland
Parallel
26
2662
100.0%
75
2.8%
2396
90.0%
147
5.5%
126
4.7%
65
2.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
26
2662
100.0%
75
2.8%
2396
90.0%
147
5.5%
126
4.7%
65
2.4%
Wales
Parallel
439
xx-xxxx-xxxx
96.3%
925
5.5%
14993
88.5%
1243
7.3%
1005
5.9%
23
0.1%
Serial/Extended
32
644
3.7%
59
9.2%
560
87.0%
45
7.0%
23
3.6%
2
0.3%
Total
471
17590
100.0%
984
5.6%
15553
88.4%
1288
7.3%
1028
5.8%
25
0.1%
GB
Parallel
1067
91701
98.0%
3699
4.0%
81632
89.0%
5826
6.4%
4614
5.0%
1756
1.9%
Serial/Extended
45
1868
2.0%
129
6.9%
1673
89.6%
104
5.6%
64
3.4%
2
0.1%
Total
1112
93569
100.0%
3828
4.1%
83305
89.0%
5930
6.3%
4678
5.0%
1758
1.9%
615.00
471.00
26.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
3
11
0.1%
1
9.1%
9
81.8%
2
18.2 %
1
9.1 %
0
Parallel - Herd or Group Slaughter
3
28
0.3%
2
7.1%
25
89.3%
1
3.6 %
1
3.6 %
0
Parallel New Infection
69
7338
67.1%
200
2.7%
6786
92.5%
458
6.2 %
349
4.8 %
3
0.0 %
Parallel - new infection cull area
24
1461
13.4%
64
4.4%
1328
90.9%
85
5.8 %
69
4.7 %
0
Parallel - Other
22
133
1.2%
23
17.3%
103
77.4%
8
6.0 %
7
5.3 %
0
Parallel Persistent Infection
16
898
8.2%
58
6.5%
772
86.0%
82
9.1 %
68
7.6 %
0
Parallel - persistent infection cull area
3
917
8.4%
32
3.5%
860
93.8%
35
3.8 %
25
2.7 %
0
Parallel - Rapid Testing Two-times IRs
44
142
1.3%
28
19.7%
99
69.7%
16
11.3 %
15
10.6 %
0
Total
184
10928
100.0%
408
3.7%
9982
91.3%
687
6.3 %
535
4.9 %
3
0.0 %
Table 4a. Summaryof IFN Gamma results by submission reason (April 2020)
England - HRA
Parallel - new infection cull area
24
1461
13.4%
64
4.4%
1328
90.9%
85
5.8 %
69
4.7 %
0
Parallel Persistent Infection
2
616
5.6%
42
6.8%
523
84.9%
64
10.4 %
51
8.3 %
0
Parallel - persistent infection cull area
3
917
8.4%
32
3.5%
860
93.8%
35
3.8 %
25
2.7 %
0
England - HRA
29
2994
27.4%
138
4.6%
2711
90.5%
184
6.1 %
145
4.8 %
0
England - Edge
Parallel New Infection
33
3946
36.1%
111
2.8%
3630
92.0%
260
6.6 %
205
5.2 %
0
England - Edge
33
3946
36.1%
111
2.8%
3630
92.0%
260
6.6 %
205
5.2 %
0
England - LRA
Parallel New Infection
5
542
5.0%
5
0.9%
508
93.7%
38
7.0 %
29
5.4 %
0
England - LRA
5
542
5.0%
5
0.9%
508
93.7%
38
7.0 %
29
5.4 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel - Herd or Group Slaughter
3
28
0.3%
2
7.1%
25
89.3%
1
3.6 %
1
3.6 %
0
Parallel - Other
18
111
1.0%
20
18.0%
85
76.6%
7
6.3 %
6
5.4 %
0
Parallel Persistent Infection
14
282
2.6%
16
5.7%
249
88.3%
18
6.4 %
17
6.0 %
0
Parallel - Rapid Testing Two-times IRs
39
126
1.2%
27
21.4%
87
69.0%
13
10.3 %
12
9.5 %
0
Wales - High TB
75
548
5.0%
65
11.9%
446
81.4%
40
7.3 %
37
6.8 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
10
0.1%
1
10.0%
9
90.0%
1
10.0 %
0
0
Parallel New Infection
13
1085
9.9%
60
5.5%
965
88.9%
74
6.8 %
59
5.4 %
1
0.1 %
Parallel - Other
3
17
0.2%
3
17.6%
13
76.5%
1
5.9 %
1
5.9 %
0
Parallel - Rapid Testing Two-times IRs
3
4
0.0%
1
25.0%
3
75.0%
0
0
0
Wales - Intermediate TB
21
1116
10.2%
65
5.8%
990
88.7%
76
6.8 %
60
5.4 %
1
0.1 %
Wales - Low TB
Parallel New Infection
13
1303
11.9%
15
1.2%
1244
95.5%
68
5.2 %
42
3.2 %
2
0.2 %
Parallel - Other
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
2
12
0.1%
0
0.0 %
9
75.0%
3
25.0 %
3
25.0 %
0
Wales - Low TB
16
1320
12.1%
15
1.1%
1258
95.3%
71
5.4 %
45
3.4 %
2
0.2 %
Scotland - Scotland
Parallel New Infection
5
462
4.2%
9
1.9%
439
95.0%
18
3.9 %
14
3.0 %
0
Scotland - Scotland
5
462
4.2%
9
1.9%
439
95.0%
18
3.9 %
14
3.0 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
45
1868
2.0%
129
6.9%
1673
89.6%
104
5.6 %
64
3.4 %
2
0.1 %
Parallel - Herd or Group Slaughter
21
307
0.3%
29
9.4%
268
87.3%
21
6.8 %
10
3.3 %
0
Parallel New Infection
376
42815
45.8%
1197
2.8%
38177
89.2%
2693
6.3 %
2167
5.1 %
1274
3.0 %
Parallel - new infection cull area
265
29044
31.0%
1180
4.1%
26014
89.6%
1784
6.1 %
1391
4.8 %
459
1.6 %
Parallel - Other
76
1755
1.9%
152
8.7%
1471
83.8%
153
8.7 %
132
7.5 %
0
Parallel Persistent Infection
186
12507
13.4%
757
6.1%
11042
88.3%
871
7.0 %
686
5.5 %
22
0.2 %
Parallel - persistent infection cull area
24
4832
5.2%
291
6.0%
4339
89.8%
274
5.7 %
201
4.2 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
119
441
0.5%
93
21.1%
321
72.8%
30
6.8 %
27
6.1 %
0
Total
1112
93569
100.0%
3828
4.1%
83305
89.0%
5930
6.3 %
4678
5.0 %
1758
1.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
13
1224
1.3%
70
5.7%
1113
90.9%
59
4.8 %
41
3.3 %
0
Parallel New Infection
5
415
0.4%
15
3.6%
363
87.5%
49
11.8 %
36
8.7 %
1
0.2 %
Parallel - new infection cull area
261
28630
30.6%
1164
4.1%
25645
89.6%
1735
6.1 %
1364
4.8 %
457
1.6 %
Parallel Persistent Infection
32
5574
6.0%
283
5.1%
5025
90.2%
348
6.2 %
263
4.7 %
3
0.1 %
Parallel - persistent infection cull area
24
4832
5.2%
291
6.0%
4339
89.8%
274
5.7 %
201
4.2 %
1
0.0 %
England - Edge
Parallel New Infection
251
30443
32.5%
890
2.9%
27138
89.1%
1899
6.2 %
1525
5.0 %
890
2.9 %
Parallel - new infection cull area
4
414
0.4%
16
3.9%
369
89.1%
49
11.8 %
27
6.5 %
2
0.5 %
England - LRA
Parallel New Infection
25
1785
1.9%
40
2.2%
1364
76.4%
82
4.6 %
67
3.8 %
314
17.6 %
England
615
73317
78.4%
2769
3.8%
65356
89.1%
4495
6.1 %
3524
4.8 %
1668
2.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
20
454
0.5%
42
9.3%
398
87.7%
31
6.8 %
14
3.1 %
0
Parallel - Herd or Group Slaughter
18
277
0.3%
24
8.7%
244
88.1%
18
6.5 %
9
3.2 %
0
Parallel New Infection
1
8
0.0%
2
25.0%
6
75.0%
0
0
0
Parallel - Other
68
1704
1.8%
147
8.6%
1429
83.9%
147
8.6 %
128
7.5 %
0
Parallel Persistent Infection
138
6211
6.6%
386
6.2%
5415
87.2%
484
7.8 %
391
6.3 %
19
0.3 %
Parallel - Rapid Testing Two-times IRs
104
397
0.4%
86
21.7%
289
72.8%
25
6.3 %
22
5.5 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
12
190
0.2%
17
8.9%
162
85.3%
14
7.4 %
9
4.7 %
2
1.1 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2020) Cont.
Parallel New Infection
43
4849
5.2%
143
2.9%
4402
90.8%
351
7.2 %
303
6.2 %
1
0.0 %
Parallel - Other
6
45
0.0%
5
11.1%
37
82.2%
5
11.1 %
3
6.7 %
0
Parallel Persistent Infection
15
682
0.7%
88
12.9%
570
83.6%
29
4.3 %
24
3.5 %
0
Parallel - Rapid Testing Two-times IRs
12
31
0.0%
7
22.6%
22
71.0%
2
6.5 %
2
6.5 %
0
Wales - Low TB
Parallel New Infection
25
2653
2.8%
32
1.2%
2508
94.5%
165
6.2 %
110
4.1 %
3
0.1 %
Parallel - Other
2
6
0.0%
0
0.0 %
5
83.3%
1
16.7 %
1
16.7 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
3
13
0.0%
0
0.0 %
10
76.9%
3
23.1 %
3
23.1 %
0
Wales
471
17590
18.8%
984
5.6%
15553
88.4%
1288
7.3 %
1028
5.8 %
25
0.1 %
Scotland - Scotland
Parallel New Infection
26
2662
2.8%
75
2.8%
2396
90.0%
147
5.5 %
126
4.7 %
65
2.4 %
Scotland
26
2662
2.8%
75
2.8%
2396
90.0%
147
5.5 %
126
4.7 %
65
2.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
1
0
4
0
773
0
73
0
0
July
316
32549
201
1219
1
13
0
0
0
0
108
3
0
12
0
1020
0
58
0
2
August
262
21887
126
724
1
8
0
0
0
0
84
1
0
6
0
570
0
52
0
0
September
245
25317
134
693
1
7
0
0
0
0
94
1
0
3
0
542
0
45
0
0
October
298
26690
154
743
0
0
0
0
0
58
1
0
5
0
604
0
71
0
3
November
272
30186
169
1080
14
0
0
0
0
72
5
0
15
0
910
0
61
0
1
December
234
25192
138
904
2
0
0
0
0
0
87
1
0
4
1
759
0
49
0
1
2020 January
343
31274
227
1367
1
7
0
0
0
0
178
3
0
8
0
1110
1
57
0
0
February
323
33146
192
1289
1
21
0
0
0
0
187
0
0
3
6
982
2
87
0
0
March
262
18221
147
764
1
28
0
0
0
0
100
0
6
2
0
591
35
1
0
0
April
184
10928
96
408
2
138
0
0
0
0
28
0
1
1
0
216
22
0
0
0
Totals (last
12 months)
3390
310034
1919
11271
15
251
0
0
0
0
1189
16
7
68
7
9039
66
596
0
7
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1951
245696
1194
8276
158
0
0
0
0
1049
8
2
43
2
6935
4
54
0
1
Scotland
45
4989
25
161
4
0
0
0
0
24
0
0
4
0
111
15
2
0
0
Wales
1394
59349
700
2834
89
0
0
0
0
116
8
5
21
5
1993
47
540
0
6
Totals
3390
310034
1919
11271
251
0
0
0
0
1189
16
7
68
7
9039
66
596
0
7
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
80
Wigtown
Warks
S Wales
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
N Yorks
Northants
NE Wales
Leics & Rut
Kirkcudbright
Gwynedd
Gwent
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Berks
Argyll & Bute
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during April 2020 (% submission per County)
County
%Resampled %Retested
Argyll & Bute
37.50 %
37.50 %
Berks
6.77 %
8.83 %
Bucks
8.33 %
12.50 %
Carms
5.52 %
6.63 %
Ceredigion
16.13 %
16.13 %
Cheshire
5.01 %
6.22 %
Corn & Scilly
2.39 %
3.53 %
Derbyshire
3.83 %
4.14 %
Devon
4.95 %
6.07 %
Dorset
7.15 %
9.09 %
E Sussex
5.06 %
5.52 %
Gloucs
4.95 %
5.45 %
Gr Manchester
Gwent
Gwynedd
3.48 %
5.54 %
Kirkcudbright
2.35 %
3.53 %
Leics & Rut
3.67 %
8.72 %
NE Wales
4.80 %
6.58 %
Northants
2.78 %
5.56 %
N Yorks
5.37 %
7.04 %
Oxon
8.33 %
10.42 %
Pembs
6.87 %
7.22 %
Powys
4.20 %
4.20 %
S Glamorgan
Shropshire
Somerset excl
5.00 %
5.00 %
S Wales
Warks
6.92 %
8.85 %
Wigtown
1.66 %
2.49 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
7439
254
3.4%
7482
254
3.4%
Wales
2923
145
5.0%
2984
145
4.9%
Scotland
461
9
2.0%
462
9
1.9%
GB
10823
408
3.8%
10928
408
3.7%
Table 6a (i): Number of animals and submissions tested in April 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
69064
2769
4.0%
73317
2769
3.8%
Wales
16751
983
5.9%
17590
984
5.6%
Scotland
2517
75
3.0%
2662
75
2.8%
GB
88332
3827
4.3%
93569
3828
4.1%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of April
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
23451
918
3.9%
23776
918
3.9%
Wales
6535
217
3.3%
6544
217
3.3%
GB
29986
1135
3.8%
30320
1135
3.7%
Table 6a (ii): Number of animals and submissions tested in April 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of April
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
86743
4519
5.2%
91395
4519
4.9%
Wales
22260
1348
6.1%
23740
1348
5.7%
Scotland
366
4
1.1%
366
4
1.1%
GB
109369
5871
5.4%
115501
5871
5.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples April 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
1
3
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Oxfordshire
PRV-SP
1
32
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
9
195
3
1.54 %
Isle of Man
SER-IOM
8
46
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
4
29
5
17.2%
Ceredigion
3
19
3
15.8%
North East Wales
2
10
1
10.0%
Pembrokeshire
12
86
22
25.6%
Powys
2
20
2
10.0%
South Wales
2
7
4
57.1%
TC0077
25
171
37
21.6 %
Table 8a Number of Cattle Antibody Tests April 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
12
245
37
15.1%
Ceredigion
7
26
4
15.4%
Devon
4
1,032
95
9.2%
North East Wales
14
515
55
10.7%
Pembrokeshire
28
443
78
17.6%
Powys
4
103
13
12.6%
South Wales
10
149
34
22.8%
TC0077
79
2,513
316
12.6 %
Table 8b Number of Cattle Antibody Tests 2020