Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - July 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 155
Report Period 1st - 31st July 2019
Issued 27 August 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
July 2019
%
Total 2019
%
Submissions
316
2160
Samples
Total (%)
32549
100.00 %
202694
100.00 %
England
25006
76.83 %
161696
79.77 %
Wales
6558
20.15 %
39142
19.31 %
Scotland
985
3.03 %
1856
0.92 %
Parallel Tests
PAR-CUL-N
6170
18.96 %
42147
20.79 %
PAR-CUL-P
2137
6.57 %
11493
5.67 %
PAR-HERD-S
33
0.10 %
4077
2.01 %
PAR-NEW-IN
13685
42.04 %
95385
47.06 %
PAR-OTHER
396
1.22 %
3733
1.84 %
PAR-PERSIS
8966
27.55 %
40038
19.75 %
PAR-RAPID
83
0.26 %
809
0.40 %
Total(% of all samples)
31470
96.68 %
197682
97.53 %
Serial/Extended Tests
SER-FLEXI
1079
3.32 %
4986
2.46 %
SER-FRAUD
0
0.00 %
18
0.01 %
SER-NSR
0
0.00 %
8
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
1079
3.32 %
5012
2.47 %
Total
32549
100.00 %
202694
100.00 %
Retests
Total (% of all samples)
2283
7.01 %
13897
6.86 %
England
1963
6.03 %
11372
5.61 %
Wales
270
0.83 %
2437
1.20 %
Scotland
50
0.15 %
88
0.04 %
Resamples
Total (% of all samples)
1669
5.13 %
10962
5.41 %
England
1444
4.44 %
8963
4.42 %
Wales
183
0.56 %
1926
0.95 %
Scotland
42
0.13 %
73
0.04 %
Rejects
Total (% of all samples)
3
0.01 %
1887
0.93 %
England
3
0.01 %
1709
0.84 %
Wales
0
0.00 %
178
0.09 %
Scotland
0
0.00 %
Table 1. Monthly Totals for July 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
6170
19.0 %
PAR-CUL-P
2137
6.6 %
PAR-HERD-S
33
0.1 %
PAR-NEW-IN
13685
42.0 %
PAR-OTHER
396
1.2 %
PAR-PERSIS
8966
27.5 %
PAR-RAPID
83
0.3 %
SER-FLEXI
1079
3.3 %
Total
32549
100.00 %
Figure 1. Number of samples submitted in each category during July 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
Wigtown
Dumfries & G
Argyll & Bute
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Warks
S Yorks
Somerset excl N
Shropshire
Oxon
Northants
Leics & Rut
Kent
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during July 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Wiltshire
Warks
S Yorks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Leicestershire and Rutland
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
cotland
Wales
ngland
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
1
222
0.68 %
8
3.60 %
208
93.69 %
11
4.95 %
6
2.70 %
0
0.00 %
PAR-OTHER
1
21
0.06 %
1
4.76 %
13
61.90 %
7
33.33 %
7
33.33 %
0
0.00 %
PAR-PERSIS
3
445
1.37 %
17
3.82 %
414
93.03 %
22
4.94 %
14
3.15 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
7
1,292
3.97 %
32
2.48 %
1169
90.48 %
109
8.44 %
90
6.97 %
1
0.08 %
PAR-PERSIS
6
1,932
5.94 %
100
5.18 %
1766
91.41 %
89
4.61 %
66
3.42 %
0
0.00 %
Devon
PAR-CUL-N
27
3,075
9.45 %
194
6.31 %
2754
89.56 %
178
5.79 %
127
4.13 %
0
0.00 %
PAR-NEW-IN
1
93
0.29 %
10
10.75 %
71
76.34 %
17
18.28 %
12
12.90 %
0
0.00 %
PAR-PERSIS
1
4
0.01 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
27
0.08 %
3
11.11 %
23
85.19 %
3
11.11 %
1
3.70 %
0
0.00 %
Dorset
PAR-CUL-N
8
373
1.15 %
36
9.65 %
311
83.38 %
34
9.12 %
26
6.97 %
0
0.00 %
PAR-CUL-P
3
299
0.92 %
51
17.06 %
231
77.26 %
17
5.69 %
17
5.69 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gloucs
PAR-CUL-N
7
349
1.07 %
9
2.58 %
309
88.54 %
56
16.05 %
31
8.88 %
0
0.00 %
PAR-CUL-P
3
811
2.49 %
42
5.18 %
705
86.93 %
83
10.23 %
64
7.89 %
0
0.00 %
SER-FLEXI
2
30
0.09 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
1
242
0.74 %
10
4.13 %
221
91.32 %
16
6.61 %
11
4.55 %
0
0.00 %
PAR-NEW-IN
1
11
0.03 %
0
0.00 %
10
90.91 %
4
36.36 %
1
9.09 %
0
0.00 %
PAR-PERSIS
4
299
0.92 %
7
2.34 %
276
92.31 %
22
7.36 %
16
5.35 %
0
0.00 %
Shropshire
PAR-PERSIS
2
511
1.57 %
12
2.35 %
472
92.37 %
30
5.87 %
26
5.09 %
1
0.20 %
Somerset excl N
PAR-CUL-N
3
415
1.28 %
18
4.34 %
380
91.57 %
32
7.71 %
17
4.10 %
0
0.00 %
PAR-PERSIS
3
445
1.37 %
10
2.25 %
418
93.93 %
23
5.17 %
17
3.82 %
0
0.00 %
Wiltshire
PAR-CUL-N
7
424
1.30 %
18
4.25 %
376
88.68 %
36
8.49 %
30
7.08 %
0
0.00 %
PAR-CUL-P
3
805
2.47 %
34
4.22 %
735
91.30 %
47
5.84 %
36
4.47 %
0
0.00 %
PAR-PERSIS
2
854
2.62 %
19
2.22 %
806
94.38 %
43
5.04 %
29
3.40 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
6
568
1.75 %
23
4.05 %
496
87.32 %
63
11.09 %
49
8.63 %
0
0.00 %
Bucks
PAR-NEW-IN
5
580
1.78 %
21
3.62 %
496
85.52 %
66
11.38 %
63
10.86 %
0
0.00 %
Table 2a. Summary by County for July 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for July 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Cheshire
PAR-NEW-IN
19
4,370
13.43 %
78
1.78 %
4131
94.53 %
295
6.75 %
160
3.66 %
1
0.02 %
PAR-PERSIS
1
14
0.04 %
0
0.00 %
8
57.14 %
7
50.00 %
6
42.86 %
0
0.00 %
Derbyshire
PAR-NEW-IN
20
2,546
7.82 %
88
3.46 %
2212
86.88 %
280
11.00 %
246
9.66 %
0
0.00 %
E Sussex
PAR-NEW-IN
4
211
0.65 %
1
0.47 %
196
92.89 %
20
9.48 %
30
14.22 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
423
1.30 %
5
1.18 %
398
94.09 %
25
5.91 %
20
4.73 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
976
3.00 %
36
3.69 %
887
90.88 %
77
7.89 %
53
5.43 %
0
0.00 %
Northants
PAR-NEW-IN
2
65
0.20 %
5
7.69 %
57
87.69 %
7
10.77 %
3
4.62 %
0
0.00 %
Oxon
PAR-NEW-IN
12
1,144
3.51 %
25
2.19 %
1054
92.13 %
100
8.74 %
65
5.68 %
0
0.00 %
Warks
PAR-NEW-IN
8
167
0.51 %
4
2.40 %
144
86.23 %
21
12.57 %
19
11.38 %
0
0.00 %
PAR-PERSIS
1
285
0.88 %
7
2.46 %
268
94.04 %
16
5.61 %
10
3.51 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
1
170
0.52 %
15
8.82 %
144
84.71 %
14
8.24 %
11
6.47 %
0
0.00 %
Cumbria
PAR-NEW-IN
2
303
0.93 %
2
0.66 %
282
93.07 %
37
12.21 %
19
6.27 %
0
0.00 %
Kent
PAR-NEW-IN
1
10
0.03 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
S Yorks
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Sussex
PAR-NEW-IN
1
193
0.59 %
2
1.04 %
145
75.13 %
51
26.42 %
46
23.83 %
0
0.00 %
England
194
25006
76.83 %
948
3.79 %
22627
90.49 %
1963
7.85 %
1444
5.77 %
3
0.01 %
Wales - High TB
Carms
PAR-HERD-S
3
19
0.06 %
3
15.79 %
13
68.42 %
5
26.32 %
3
15.79 %
0
0.00 %
PAR-OTHER
3
66
0.20 %
8
12.12 %
56
84.85 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-PERSIS
6
408
1.25 %
26
6.37 %
373
91.42 %
13
3.19 %
9
2.21 %
0
0.00 %
PAR-RAPID
8
21
0.06 %
1
4.76 %
19
90.48 %
1
4.76 %
1
4.76 %
0
0.00 %
SER-FLEXI
2
154
0.47 %
12
7.79 %
141
91.56 %
3
1.95 %
1
0.65 %
0
0.00 %
Ceredigion
PAR-OTHER
1
147
0.45 %
4
2.72 %
136
92.52 %
9
6.12 %
7
4.76 %
0
0.00 %
PAR-PERSIS
5
338
1.04 %
8
2.37 %
323
95.56 %
16
4.73 %
7
2.07 %
0
0.00 %
PAR-RAPID
4
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
3
522
1.60 %
27
5.17 %
479
91.76 %
26
4.98 %
16
3.07 %
0
0.00 %
Pembs
PAR-OTHER
2
158
0.49 %
12
7.59 %
137
86.71 %
11
6.96 %
9
5.70 %
0
0.00 %
PAR-PERSIS
15
2,260
6.94 %
43
1.90 %
2165
95.80 %
77
3.41 %
52
2.30 %
0
0.00 %
PAR-RAPID
15
25
0.08 %
3
12.00 %
20
80.00 %
3
12.00 %
2
8.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for July 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
10
368
1.13 %
25
6.79 %
333
90.49 %
19
5.16 %
10
2.72 %
0
0.00 %
Powys
PAR-HERD-S
1
7
0.02 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
220
0.68 %
1
0.45 %
202
91.82 %
19
8.64 %
17
7.73 %
0
0.00 %
PAR-RAPID
7
15
0.05 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
249
0.77 %
15
6.02 %
232
93.17 %
3
1.20 %
2
0.80 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
1
7
0.02 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
5
0.02 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
20
0.06 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-OTHER
1
4
0.01 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
664
2.04 %
20
3.01 %
631
95.03 %
21
3.16 %
13
1.96 %
0
0.00 %
PAR-RAPID
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
113
0.35 %
1
0.88 %
108
95.58 %
4
3.54 %
4
3.54 %
0
0.00 %
Powys
PAR-NEW-IN
1
12
0.04 %
1
8.33 %
9
75.00 %
5
41.67 %
2
16.67 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
8
0.02 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
30
0.09 %
6
20.00 %
23
76.67 %
2
6.67 %
1
3.33 %
0
0.00 %
S Wales
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
112
0.34 %
2
1.79 %
108
96.43 %
3
2.68 %
2
1.79 %
0
0.00 %
NE Wales
PAR-NEW-IN
2
81
0.25 %
1
1.23 %
74
91.36 %
6
7.41 %
6
7.41 %
0
0.00 %
PAR-PERSIS
2
429
1.32 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
SER-FLEXI
1
87
0.27 %
4
4.60 %
81
93.10 %
3
3.45 %
2
2.30 %
0
0.00 %
Wales
117
6558
20.15 %
249
3.80 %
6126
93.41 %
270
4.12 %
183
2.79 %
0
0.00 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.12 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
627
1.93 %
10
1.59 %
601
95.85 %
18
2.87 %
16
2.55 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
319
0.98 %
11
3.45 %
282
88.40 %
32
10.03 %
26
8.15 %
0
0.00 %
Scotland
5
985
3.03 %
22
2.23 %
921
93.50 %
50
5.08 %
42
4.26 %
0
0.00 %
Grand Total
Sum:
316
32549
1219
29674
2283
1669
3
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
3
431
0.21 %
9
2.09 %
407
94.43 %
23
5.34 %
15
3.48 %
0
0.00 %
PAR-OTHER
1
21
0.01 %
1
4.76 %
13
61.90 %
7
33.33 %
7
33.33 %
0
0.00 %
PAR-PERSIS
9
1,490
0.74 %
45
3.02 %
1387
93.09 %
72
4.83 %
56
3.76 %
2
0.13 %
SER-FRAUD
2
18
0.01 %
0
0.00 %
17
94.44 %
1
5.56 %
1
5.56 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
36
3,913
1.93 %
121
3.09 %
3496
89.34 %
301
7.69 %
245
6.26 %
51
1.30 %
PAR-CUL-P
4
1,165
0.57 %
19
1.63 %
1035
88.84 %
132
11.33 %
109
9.36 %
2
0.17 %
PAR-NEW-IN
2
124
0.06 %
6
4.84 %
101
81.45 %
18
14.52 %
17
13.71 %
0
0.00 %
PAR-PERSIS
24
5,103
2.52 %
229
4.49 %
4380
85.83 %
268
5.25 %
199
3.90 %
295
5.78 %
SER-FLEXI
2
15
0.01 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
137
18,272
9.01 %
1056
5.78 %
16294
89.17 %
1091
5.97 %
815
4.46 %
107
0.59 %
PAR-CUL-P
20
4,525
2.23 %
286
6.32 %
3980
87.96 %
301
6.65 %
255
5.64 %
4
0.09 %
PAR-NEW-IN
4
674
0.33 %
106
15.73 %
501
74.33 %
86
12.76 %
67
9.94 %
0
0.00 %
PAR-OTHER
7
532
0.26 %
6
1.13 %
352
66.17 %
202
37.97 %
174
32.71 %
0
0.00 %
PAR-PERSIS
19
2,346
1.16 %
162
6.91 %
2038
86.87 %
190
8.10 %
145
6.18 %
1
0.04 %
SER-FLEXI
8
849
0.42 %
50
5.89 %
732
86.22 %
47
5.54 %
22
2.59 %
45
5.30 %
Dorset
PAR-CUL-N
71
8,939
4.41 %
482
5.39 %
7956
89.00 %
566
6.33 %
445
4.98 %
56
0.63 %
PAR-CUL-P
10
1,679
0.83 %
137
8.16 %
1475
87.85 %
75
4.47 %
67
3.99 %
0
0.00 %
PAR-NEW-IN
2
36
0.02 %
1
2.78 %
27
75.00 %
8
22.22 %
8
22.22 %
0
0.00 %
SER-FLEXI
4
153
0.08 %
6
3.92 %
137
89.54 %
11
7.19 %
10
6.54 %
0
0.00 %
Gloucs
PAR-CUL-N
42
2,849
1.41 %
119
4.18 %
2419
84.91 %
256
8.99 %
203
7.13 %
108
3.79 %
PAR-CUL-P
6
1,112
0.55 %
44
3.96 %
987
88.76 %
100
8.99 %
81
7.28 %
0
0.00 %
PAR-HERD-S
4
33
0.02 %
0
0.00 %
31
93.94 %
3
9.09 %
2
6.06 %
0
0.00 %
SER-FLEXI
2
30
0.01 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
14
1,553
0.77 %
67
4.31 %
1375
88.54 %
125
8.05 %
100
6.44 %
11
0.71 %
PAR-CUL-P
2
182
0.09 %
4
2.20 %
159
87.36 %
22
12.09 %
19
10.44 %
0
0.00 %
PAR-NEW-IN
1
11
0.01 %
0
0.00 %
10
90.91 %
4
36.36 %
1
9.09 %
0
0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
23
3,167
1.56 %
126
3.98 %
2849
89.96 %
235
7.42 %
186
5.87 %
6
0.19 %
Shropshire
PAR-CUL-P
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
525
0.26 %
38
7.24 %
448
85.33 %
45
8.57 %
38
7.24 %
1
0.19 %
PAR-PERSIS
7
716
0.35 %
19
2.65 %
636
88.83 %
66
9.22 %
60
8.38 %
1
0.14 %
SER-FLEXI
5
557
0.27 %
21
3.77 %
509
91.38 %
34
6.10 %
27
4.85 %
0
0.00 %
Somerset excl N
PAR-CUL-N
24
2,478
1.22 %
144
5.81 %
2221
89.63 %
150
6.05 %
111
4.48 %
2
0.08 %
PAR-OTHER
2
238
0.12 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
13
1,907
0.94 %
110
5.77 %
1510
79.18 %
105
5.51 %
69
3.62 %
218
11.43 %
Staffs
PAR-PERSIS
7
1,492
0.74 %
202
13.54 %
1201
80.50 %
130
8.71 %
90
6.03 %
0
0.00 %
Wiltshire
PAR-CUL-N
41
3,883
1.92 %
174
4.48 %
3466
89.26 %
261
6.72 %
214
5.51 %
29
0.75 %
PAR-CUL-P
11
2,390
1.18 %
159
6.65 %
2141
89.58 %
124
5.19 %
89
3.72 %
1
0.04 %
PAR-NEW-IN
4
436
0.22 %
15
3.44 %
381
87.39 %
50
11.47 %
40
9.17 %
0
0.00 %
PAR-OTHER
2
37
0.02 %
1
2.70 %
34
91.89 %
4
10.81 %
2
5.41 %
0
0.00 %
PAR-PERSIS
4
1,146
0.57 %
25
2.18 %
1077
93.98 %
55
4.80 %
39
3.40 %
5
0.44 %
SER-FLEXI
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
34
4,158
2.05 %
153
3.68 %
3731
89.73 %
337
8.10 %
274
6.59 %
0
0.00 %
PAR-PERSIS
3
204
0.10 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
21
2,217
1.09 %
73
3.29 %
1964
88.59 %
207
9.34 %
165
7.44 %
15
0.68 %
Cheshire
PAR-CUL-N
1
260
0.13 %
19
7.31 %
229
88.08 %
12
4.62 %
12
4.62 %
0
0.00 %
PAR-NEW-IN
146
29,325
14.47 %
759
2.59 %
27132
92.52 %
1729
5.90 %
1283
4.38 %
151
0.51 %
PAR-PERSIS
5
540
0.27 %
3
0.56 %
512
94.81 %
29
5.37 %
25
4.63 %
0
0.00 %
Derbyshire
PAR-NEW-IN
117
12,619
6.23 %
445
3.53 %
11083
87.83 %
1025
8.12 %
878
6.96 %
213
1.69 %
E Sussex
PAR-NEW-IN
21
2,721
1.34 %
125
4.59 %
2450
90.04 %
157
5.77 %
149
5.48 %
13
0.48 %
Hampshire
PAR-NEW-IN
25
1,953
0.96 %
53
2.71 %
1788
91.55 %
131
6.71 %
112
5.73 %
0
0.00 %
SER-NSR
2
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leicestershire and
Rutland
PAR-NEW-IN
1
24
0.01 %
0
0.00 %
24
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
51
6,233
3.08 %
261
4.19 %
5528
88.69 %
430
6.90 %
349
5.60 %
95
1.52 %
Northants
PAR-NEW-IN
26
1,886
0.93 %
117
6.20 %
xx-xxxx-xxxx
89.40 %
129
6.84 %
83
4.40 %
0
0.00 %
Notts
PAR-NEW-IN
7
774
0.38 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
96
9,065
4.47 %
405
4.47 %
8016
88.43 %
752
8.30 %
594
6.55 %
50
0.55 %
Warks
PAR-NEW-IN
74
6,163
3.04 %
309
5.01 %
5388
87.42 %
484
7.85 %
382
6.20 %
84
1.36 %
PAR-PERSIS
6
570
0.28 %
19
3.33 %
526
92.28 %
32
5.61 %
25
4.39 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
6
398
0.20 %
22
5.53 %
347
87.19 %
27
6.78 %
21
5.28 %
8
2.01 %
Cumbria
PAR-NEW-IN
15
3,297
1.63 %
110
3.34 %
3001
91.02 %
250
7.58 %
186
5.64 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.05 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
79
0.04 %
6
7.59 %
52
65.82 %
25
31.65 %
21
26.58 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.50 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
4
641
0.32 %
16
2.50 %
562
87.68 %
69
10.76 %
62
9.67 %
1
0.16 %
Kent
PAR-NEW-IN
1
10
0.00 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
106
0.05 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
8
754
0.37 %
20
2.65 %
693
91.91 %
46
6.10 %
41
5.44 %
0
0.00 %
N Yorks
PAR-NEW-IN
7
875
0.43 %
22
2.51 %
766
87.54 %
71
8.11 %
59
6.74 %
28
3.20 %
SER-FLEXI
2
50
0.02 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
S Yorks
PAR-NEW-IN
5
404
0.20 %
7
1.73 %
381
94.31 %
21
5.20 %
15
3.71 %
1
0.25 %
W Sussex
PAR-NEW-IN
1
193
0.10 %
2
1.04 %
145
75.13 %
51
26.42 %
46
23.83 %
0
0.00 %
W Yorks
PAR-HERD-S
2
24
0.01 %
8
33.33 %
14
58.33 %
2
8.33 %
2
8.33 %
0
0.00 %
England
1288
161696
79.77 %
7075
4.38 %
143966
89.03 %
11372
7.03 %
8963
5.54 %
1709
1.06 %
Wales - High TB
Carms
PAR-HERD-S
43
2,321
1.15 %
152
6.55 %
2040
87.89 %
157
6.76 %
127
5.47 %
2
0.09 %
PAR-NEW-IN
3
121
0.06 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
11
429
0.21 %
53
12.35 %
369
86.01 %
16
3.73 %
7
1.63 %
0
0.00 %
PAR-PERSIS
52
5,243
2.59 %
254
4.84 %
4767
90.92 %
284
5.42 %
211
4.02 %
13
0.25 %
PAR-RAPID
53
216
0.11 %
60
27.78 %
144
66.67 %
20
9.26 %
12
5.56 %
0
0.00 %
SER-FLEXI
28
730
0.36 %
73
10.00 %
622
85.21 %
42
5.75 %
35
4.79 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.07 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
2
313
0.15 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
2
153
0.08 %
5
3.27 %
141
92.16 %
9
5.88 %
7
4.58 %
0
0.00 %
PAR-PERSIS
19
1,508
0.74 %
119
7.89 %
1302
86.34 %
113
7.49 %
87
5.77 %
0
0.00 %
PAR-RAPID
33
84
0.04 %
21
25.00 %
59
70.24 %
5
5.95 %
4
4.76 %
0
0.00 %
SER-FLEXI
3
451
0.22 %
17
3.77 %
424
94.01 %
10
2.22 %
8
1.77 %
2
0.44 %
Gwent
PAR-HERD-S
11
887
0.44 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
1
15
0.01 %
3
20.00 %
12
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
14
1,098
0.54 %
56
5.10 %
988
89.98 %
71
6.47 %
53
4.83 %
1
0.09 %
PAR-RAPID
22
64
0.03 %
15
23.44 %
44
68.75 %
5
7.81 %
4
6.25 %
1
1.56 %
SER-FLEXI
1
20
0.01 %
2
10.00 %
18
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
21
245
0.12 %
33
13.47 %
194
79.18 %
25
10.20 %
19
7.76 %
0
0.00 %
PAR-OTHER
14
1,213
0.60 %
55
4.53 %
1073
88.46 %
106
8.74 %
85
7.01 %
0
0.00 %
PAR-PERSIS
89
10,071
4.97 %
305
3.03 %
9245
91.80 %
642
6.37 %
517
5.13 %
4
0.04 %
PAR-RAPID
85
220
0.11 %
40
18.18 %
169
76.82 %
17
7.73 %
11
5.00 %
0
0.00 %
SER-FLEXI
31
779
0.38 %
53
6.80 %
696
89.35 %
43
5.52 %
30
3.85 %
0
0.00 %
Powys
PAR-HERD-S
16
202
0.10 %
49
24.26 %
138
68.32 %
13
6.44 %
11
5.45 %
4
1.98 %
PAR-NEW-IN
15
363
0.18 %
7
1.93 %
336
92.56 %
20
5.51 %
20
5.51 %
0
0.00 %
PAR-OTHER
1
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
27
1,270
0.63 %
56
4.41 %
1135
89.37 %
92
7.24 %
76
5.98 %
3
0.24 %
PAR-RAPID
44
112
0.06 %
14
12.50 %
67
59.82 %
6
5.36 %
6
5.36 %
25
22.32 %
SER-FLEXI
9
330
0.16 %
21
6.36 %
305
92.42 %
5
1.52 %
4
1.21 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.01 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.19 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.05 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.06 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-PERSIS
1
7
0.00 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
75
0.04 %
14
18.67 %
59
78.67 %
3
4.00 %
2
2.67 %
0
0.00 %
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.17 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
7
560
0.28 %
29
5.18 %
516
92.14 %
27
4.82 %
15
2.68 %
0
0.00 %
PAR-RAPID
7
17
0.01 %
2
11.76 %
14
82.35 %
1
5.88 %
1
5.88 %
0
0.00 %
SER-FLEXI
1
20
0.01 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.01 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
2
148
0.07 %
21
14.19 %
111
75.00 %
17
11.49 %
16
10.81 %
0
0.00 %
PAR-PERSIS
4
550
0.27 %
26
4.73 %
500
90.91 %
28
5.09 %
24
4.36 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
27
0.01 %
2
7.41 %
21
77.78 %
4
14.81 %
4
14.81 %
0
0.00 %
NE Wales
PAR-NEW-IN
59
4,959
2.45 %
152
3.07 %
4510
90.95 %
314
6.33 %
245
4.94 %
52
1.05 %
PAR-OTHER
2
54
0.03 %
16
29.63 %
37
68.52 %
3
5.56 %
1
1.85 %
0
0.00 %
PAR-PERSIS
2
237
0.12 %
8
3.38 %
221
93.25 %
14
5.91 %
8
3.38 %
0
0.00 %
PAR-RAPID
12
42
0.02 %
8
19.05 %
33
78.57 %
1
2.38 %
1
2.38 %
0
0.00 %
SER-FLEXI
26
726
0.36 %
60
8.26 %
580
79.89 %
39
5.37 %
33
4.55 %
53
7.30 %
Powys
PAR-NEW-IN
10
809
0.40 %
25
3.09 %
727
89.86 %
64
7.91 %
57
7.05 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
7
34
0.02 %
3
8.82 %
31
91.18 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
59
0.03 %
10
xx-xxxx-xxxx %
48
81.36 %
2
3.39 %
1
1.69 %
0
0.00 %
S Wales
PAR-RAPID
2
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.01 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
9
272
0.13 %
10
3.68 %
254
93.38 %
10
3.68 %
7
2.57 %
1
0.37 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
12
407
0.20 %
6
1.47 %
371
91.15 %
21
5.16 %
15
3.69 %
15
3.69 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
429
0.21 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
87
0.04 %
4
4.60 %
81
93.10 %
3
3.45 %
2
2.30 %
0
0.00 %
Wales
859
39142
19.31 %
2014
5.15 %
35027
89.49 %
2437
6.23 %
1926
4.92 %
178
0.45 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.02 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
366
0.18 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
627
0.31 %
10
1.59 %
601
95.85 %
18
2.87 %
16
2.55 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
3
404
0.20 %
55
13.61 %
337
83.42 %
14
3.47 %
12
2.97 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
420
0.21 %
11
2.62 %
381
90.71 %
36
8.57 %
28
6.67 %
0
0.00 %
Scotland
13
1856
0.92 %
81
4.36 %
1702
91.70 %
88
4.74 %
73
3.93 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
9170
180695
13897
10962
1887
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol July 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
190
24948
99.8%
943
3.8%
22575
90.5%
1958
7.8%
1443
5.8%
3
0.0%
Serial/Extended
4
58
0.2%
5
8.6%
52
89.7%
5
8.6%
1
1.7%
0
0.0 %
Total
194
25006
100.0%
948
3.8%
22627
90.5%
1963
7.9%
1444
5.8%
3
0.0%
Scotland
Parallel
5
985
100.0%
22
2.2%
921
93.5%
50
5.1%
42
4.3%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
5
985
100.0%
22
2.2%
921
93.5%
50
5.1%
42
4.3%
0
0.0 %
Wales
Parallel
96
5537
84.4%
174
3.1%
5200
93.9%
236
4.3%
163
2.9%
0
0.0 %
Serial/Extended
21
1021
15.6%
75
7.3%
926
90.7%
34
3.3%
20
2.0%
0
0.0 %
Total
117
6558
100.0%
249
3.8%
6126
93.4%
270
4.1%
183
2.8%
0
0.0 %
GB
Parallel
291
31470
96.7%
1139
3.6%
28696
91.2%
2244
7.1%
xx-xxxx-xxxx
5.2%
3
0.0%
Serial/Extended
25
1079
3.3%
80
7.4%
978
90.6%
39
3.6%
21
1.9%
0
0.0 %
Total
316
32549
100.0%
1219
3.7%
29674
91.2%
2283
7.0%
1669
5.1%
3
0.0%
194.00
117.00
5.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
1260 160014
99.0%
6995
4.4% 142471
89.0%
11273
7.0%
8901
5.6%
1664
1.0%
Serial/Extended
28
xx-xxxx-xxxx
1.0%
80
4.8%
1495
88.9%
99
5.9%
62
3.7%
45
2.7%
Total
1288 161696
100.0%
7075
4.4% 143966
89.0%
11372
7.0%
8963
5.5%
1709
1.1%
Scotland
Parallel
13
1856
100.0%
81
4.4%
1702
91.7%
88
4.7%
73
3.9%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
13
1856
100.0%
81
4.4%
1702
91.7%
88
4.7%
73
3.9%
0
0.0 %
Wales
Parallel
751
35812
91.5%
1755
4.9%
32132
89.7%
2284
6.4%
1805
5.0%
123
0.3%
Serial/Extended
108
3330
8.5%
259
7.8%
2895
86.9%
153
4.6%
121
3.6%
55
1.7%
Total
859
39142
100.0%
2014
5.1%
35027
89.5%
2437
6.2%
1926
4.9%
178
0.5%
GB
Parallel
xx-xxxx-xxxx
97.5%
8831
4.5% 176305
89.2%
13645
6.9%
10779
5.5%
1787
0.9%
Serial/Extended
136
5012
2.5%
339
6.8%
4390
87.6%
252
5.0%
183
3.7%
100
2.0%
Total
xx-xxxx-xxxx
100.0%
9170
4.5% 180695
89.1%
13897
6.9%
10962
5.4%
1887
0.9%
#######
859.00
13.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
25
1079
3.3%
80
7.4%
978
90.6%
39
3.6 %
21
1.9 %
0
Parallel - Herd or Group Slaughter
5
33
0.1%
8
24.2%
22
66.7%
5
15.2 %
3
9.1 %
0
Parallel New Infection
113
13685
42.0%
362
2.6%
12476
91.2%
1165
8.5 %
862
6.3 %
1
0.0 %
Parallel - new infection cull area
60
6170
19.0%
317
5.1%
5520
89.5%
461
7.5 %
332
5.4 %
1
0.0 %
Parallel - Other
8
396
1.2%
25
6.3%
344
86.9%
31
7.8 %
27
6.8 %
0
Parallel Persistent Infection
57
8966
27.5%
286
3.2%
8381
93.5%
420
4.7 %
298
3.3 %
1
0.0 %
Parallel - persistent infection cull area
10
2137
6.6%
135
6.3%
1879
87.9%
158
7.4 %
123
5.8 %
0
Parallel - Rapid Testing Two-times IRs
38
83
0.3%
6
7.2%
74
89.2%
4
4.8 %
3
3.6 %
0
Total
316
32549
100.0%
1219
3.7%
29674
91.2%
2283
7.0 %
1669
5.1 %
3
0.0 %
Table 4a. Summaryof IFN Gamma results by submission reason (July 2019)
England - HRA
Extended Flexible IFN-GAMMA test
4
58
0.2%
5
8.6%
52
89.7%
5
8.6 %
1
1.7 %
0
Parallel New Infection
2
104
0.3%
10
9.6%
81
77.9%
21
20.2 %
13
12.5 %
0
Parallel - new infection cull area
60
6170
19.0%
317
5.1%
5520
89.5%
461
7.5 %
332
5.4 %
1
0.0 %
Parallel - Other
1
21
0.1%
1
4.8%
13
61.9%
7
33.3 %
7
33.3 %
0
Parallel Persistent Infection
21
4490
13.8%
167
3.7%
4154
92.5%
229
5.1 %
168
3.7 %
1
0.0 %
Parallel - persistent infection cull area
10
2137
6.6%
135
6.3%
1879
87.9%
158
7.4 %
123
5.8 %
0
England - HRA
98
12980
39.9%
635
4.9%
11699
90.1%
881
6.8 %
644
5.0 %
2
0.0 %
England - Edge
Parallel New Infection
88
11050
33.9%
286
2.6%
10071
91.1%
954
8.6 %
708
6.4 %
1
0.0 %
Parallel Persistent Infection
2
299
0.9%
7
2.3%
276
92.3%
23
7.7 %
16
5.4 %
0
England - Edge
90
11349
34.9%
293
2.6%
10347
91.2%
977
8.6 %
724
6.4 %
1
0.0 %
England - LRA
Parallel New Infection
6
677
2.1%
20
3.0%
581
85.8%
105
15.5 %
76
11.2 %
0
England - LRA
6
677
2.1%
20
3.0%
581
85.8%
105
15.5 %
76
11.2 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
13
771
2.4%
52
6.7%
706
91.6%
25
3.2 %
13
1.7 %
0
Parallel - Herd or Group Slaughter
4
26
0.1%
5
19.2%
18
69.2%
5
19.2 %
3
11.5 %
0
Parallel - Other
6
371
1.1%
24
6.5%
329
88.7%
22
5.9 %
18
4.9 %
0
Parallel Persistent Infection
32
3748
11.5%
105
2.8%
3542
94.5%
151
4.0 %
101
2.7 %
0
Parallel - Rapid Testing Two-times IRs
34
66
0.2%
4
6.1%
59
89.4%
4
6.1 %
3
4.5 %
0
Wales - High TB
89
4982
15.3%
190
3.8%
4654
93.4%
207
4.2 %
138
2.8 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
163
0.5%
19
11.7%
139
85.3%
6
3.7 %
5
3.1 %
0
Parallel - Herd or Group Slaughter
1
7
0.0%
3
42.9%
4
57.1%
0
0
0
Parallel New Infection
8
676
2.1%
21
3.1%
640
94.7%
26
3.8 %
15
2.2 %
0
Parallel - Other
1
4
0.0%
0
0.0 %
2
50.0%
2
50.0 %
2
50.0 %
0
Parallel - Rapid Testing Two-times IRs
4
17
0.1%
2
11.8%
15
88.2%
0
0
0
Wales - Intermediate TB
21
867
2.7%
45
5.2%
800
92.3%
34
3.9 %
22
2.5 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
87
0.3%
4
4.6%
81
93.1%
3
3.4 %
2
2.3 %
0
Parallel New Infection
4
193
0.6%
3
1.6%
182
94.3%
9
4.7 %
8
4.1 %
0
Parallel Persistent Infection
2
429
1.3%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Wales - Low TB
7
709
2.2%
14
2.0%
672
94.8%
29
4.1 %
23
3.2 %
0
Scotland - Scotland
Parallel New Infection
5
985
3.0%
22
2.2%
921
93.5%
50
5.1 %
42
4.3 %
0
Scotland - Scotland
5
985
3.0%
22
2.2%
921
93.5%
50
5.1 %
42
4.3 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
132
4986
2.5%
339
6.8%
4365
87.5%
251
5.0 %
182
3.7 %
100
2.0 %
Parallel - Herd or Group Slaughter
109
4077
2.0%
328
8.0%
3480
85.4%
317
7.8 %
262
6.4 %
8
0.2 %
Parallel New Infection
818
95385
47.1%
3438
3.6%
85745
89.9%
6814
7.1 %
5427
5.7 %
791
0.8 %
Parallel - new infection cull area
366
42147
20.8%
2182
5.2%
37456
88.9%
2762
6.6 %
2145
5.1 %
364
0.9 %
Parallel - Other
58
3733
1.8%
248
6.6%
3085
82.6%
433
11.6 %
358
9.6 %
42
1.1 %
Parallel Persistent Infection
340
40038
19.8%
1810
4.5%
35771
89.3%
2485
6.2 %
1911
4.8 %
549
1.4 %
Parallel - persistent infection cull area
57
11493
5.7%
659
5.7%
10192
88.7%
777
6.8 %
635
5.5 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
276
809
0.4%
166
20.5%
576
71.2%
57
7.0 %
41
5.1 %
26
3.2 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
9170
4.5%
180695
89.1%
13897
6.9 %
10962
5.4 %
1887
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
22
1606
0.8%
79
4.9%
1422
88.5%
95
5.9 %
60
3.7 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
14
1282
0.6%
128
10.0%
1021
79.6%
166
12.9 %
133
10.4 %
0
Parallel - new infection cull area
365
41887
20.7%
2163
5.2%
37227
88.9%
2750
6.6 %
2133
5.1 %
364
0.9 %
Parallel - Other
17
1354
0.7%
80
5.9%
1003
74.1%
266
19.6 %
229
16.9 %
42
3.1 %
Parallel Persistent Infection
106
17367
8.6%
918
5.3%
15078
86.8%
1121
6.5 %
844
4.9 %
528
3.0 %
Parallel - persistent infection cull area
57
11493
5.7%
659
5.7%
10192
88.7%
777
6.8 %
635
5.5 %
7
0.1 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
619
77138
38.1%
2736
3.5%
69408
90.0%
5450
7.1 %
4325
5.6 %
685
0.9 %
Parallel - new infection cull area
1
260
0.1%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
14
1314
0.6%
27
2.1%
1234
93.9%
66
5.0 %
53
4.0 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
61
7862
3.9%
257
3.3%
7034
89.5%
660
8.4 %
533
6.8 %
38
0.5 %
England
1288 161696
79.8%
7075
4.4%
143966
89.0%
11372
7.0 %
8963
5.5 %
1709
1.1 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Wales - High TB
Extended Flexible IFN-GAMMA test
75
2411
1.2%
171
7.1%
2157
89.5%
105
4.4 %
81
3.4 %
2
0.1 %
Parallel - Herd or Group Slaughter
97
3919
1.9%
303
7.7%
3363
85.8%
299
7.6 %
246
6.3 %
8
0.2 %
Parallel New Infection
20
797
0.4%
43
5.4%
715
89.7%
41
5.1 %
39
4.9 %
0
Parallel - Other
30
1825
0.9%
117
6.4%
1609
88.2%
131
7.2 %
99
5.4 %
0
Parallel Persistent Infection
205
19581
9.7%
795
4.1%
17813
91.0%
1212
6.2 %
954
4.9 %
21
0.1 %
Parallel - Rapid Testing Two-times IRs
241
704
0.3%
150
21.3%
490
69.6%
54
7.7 %
38
5.4 %
26
3.7 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
32
832
0.4%
84
10.1%
657
79.0%
45
5.4 %
38
4.6 %
53
6.4 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
70
5771
2.8%
177
3.1%
5240
90.8%
378
6.5 %
302
5.2 %
52
0.9 %
Parallel - Other
11
554
0.3%
51
9.2%
473
85.4%
36
6.5 %
30
5.4 %
0
Parallel Persistent Infection
13
1347
0.7%
63
4.7%
1237
91.8%
69
5.1 %
47
3.5 %
0
Parallel - Rapid Testing Two-times IRs
33
103
0.1%
16
15.5%
84
81.6%
3
2.9 %
3
2.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
87
0.0%
4
4.6%
81
93.1%
3
3.4 %
2
2.3 %
0
Parallel New Infection
21
679
0.3%
16
2.4%
625
92.0%
31
4.6 %
22
3.2 %
16
2.4 %
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales
859
39142
19.3%
2014
5.1%
35027
89.5%
2437
6.2 %
1926
4.9 %
178
0.5 %
Scotland - Scotland
Parallel New Infection
13
1856
0.9%
81
4.4%
1702
91.7%
88
4.7 %
73
3.9 %
0
Scotland
13
1856
0.9%
81
4.4%
1702
91.7%
88
4.7 %
73
3.9 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 August
228
19599
120
682
4
3
0
0
0
0
64
2
0
8
1
569
0
31
0
0
September
216
21441
137
946
4
6
0
0
0
0
33
1
0
2
0
842
0
51
1
4
October
274
23976
152
1417
1
8
0
0
0
0
100
2
0
3
0
1242
0
44
0
2
November
282
24570
192
1580
2
4
0
0
0
0
42
5
0
8
2
1336
0
169
0
10
December
228
21554
147
1241
6
8
0
0
0
0
92
2
0
7
1
1037
0
82
0
5
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
0
0
5
3
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
9
39
0
0
June
287
26287
159
964
5
18
0
0
0
0
94
0
4
1
0
768
73
0
0
0
July
316
32549
201
1219
1
330
0
0
0
0
89
0
8
0
0
744
34
0
0
0
Totals (last
12 months)
3388
313834
2040
15036
31
399
0
0
0
0
1087
20
12
55
7
12556
116
680
3
28
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1965
246558
1312
11288
321
0
0
0
0
923
12
12
34
4
9827
16
96
0
2
Scotland
17
1924
10
84
1
0
0
0
0
12
0
0
2
0
54
0
4
0
0
Wales
1406
65352
718
3664
77
0
0
0
0
152
8
0
19
3
2675
100
580
3
26
Totals
3388
313834
2040
15036
399
0
0
0
0
1087
20
12
55
7
12556
116
680
3
28
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
W Sussex
Wigtown
S Yorks
Somerset excl N
S Glamorgan
Pembs
Northants
Leics & Rut
Heref
Gwynedd
Gloucs
Dumfries & G
Devon
Cumbria
Cheshire
Carms
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during July 2019 (% submission per County)
County
%Resampled %Retested
Argyll & Bute
Avon
3.92 %
5.81 %
Berks
8.63 %
11.09 %
Bucks
10.86 %
11.38 %
Cambs
6.47 %
8.24 %
Carms
2.29 %
3.43 %
Ceredigion
3.24 %
5.47 %
Cheshire
3.79 %
6.89 %
Corn & Scilly
4.84 %
6.14 %
Cumbria
6.27 %
12.21 %
Derbyshire
9.66 %
11.00 %
Devon
4.38 %
6.19 %
Dorset
6.39 %
7.58 %
Dumfries & G
2.55 %
2.87 %
E Sussex
14.22 %
9.48 %
Gloucs
7.98 %
11.85 %
Gwent
3.07 %
4.98 %
Gwynedd
1.79 %
2.68 %
Hampshire
4.73 %
5.91 %
Heref
5.07 %
7.61 %
Kent
30.00 %
Leics & Rut
5.43 %
7.89 %
NE Wales
2.76 %
3.70 %
Northants
4.62 %
10.77 %
Oxon
5.68 %
8.74 %
Pembs
2.60 %
3.91 %
Powys
4.17 %
5.37 %
S Glamorgan
2.63 %
5.26 %
Shropshire
5.09 %
5.87 %
Somerset excl
3.95 %
6.40 %
S Wales
S Yorks
Warks
6.42 %
8.19 %
Wigtown
8.15 %
10.03 %
Wiltshire
4.56 %
6.05 %

W Sussex
23.83 %
26.42 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
24686
948
3.8%
25006
948
3.8%
Wales
6521
249
3.8%
6558
249
3.8%
Scotland
970
22
2.3%
985
22
2.2%
GB
32177
1219
3.8%
32549
1219
3.7%
Table 6a (i): Number of animals and submissions tested in July 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
151628
7075
4.7%
161696
7075
4.4%
Wales
36139
2014
5.6%
39142
2014
5.1%
Scotland
1829
81
4.4%
1856
81
4.4%
GB
189593
9170
4.8%
202694
9170
4.5%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of July
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18799
620
3.3%
18982
620
3.3%
Wales
4628
167
3.6%
4774
167
3.5%
Scotland
336
19
5.7%
355
19
5.4%
GB
23761
806
3.4%
24111
806
3.3%
Table 6a (ii): Number of animals and submissions tested in July 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of July
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
97365
5407
5.6%
108776
5407
5.0%
Wales
28831
1219
4.2%
31397
1219
3.9%
Scotland
2017
178
8.8%
2173
178
8.2%
GB
128200
6804
5.3%
142346
6804
4.8%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SP
3
3
0
0
Total
3
3
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples July 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
1
6
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Somerset excl North
PRV-SE
4
41
15
36.59 %
PRV-SP
3
3
0
0
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
12
64
15
23.44 %
Isle of Man
SER-IOM
13
52
1
1.92 %

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
2
31
3
9.7%
North East Wales
1
84
3
3.6%
Pembrokeshire
3
130
6
4.6%
Powys
1
249
11
4.4%
South Wales
1
32
2
6.3%
TC0077
Sum:
526
25
4.8 %
Table 8a Number of Cattle Antibody Tests July 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
8
281
22
7.8%
Ceredigion
1
29
7
24.1%
North East Wales
12
467
66
14.1%
Pembrokeshire
12
395
21
5.3%
Powys
4
268
14
5.2%
South Wales
4
187
16
8.6%
TC0077
Sum:
1,627
146
9.0 %
Table 8b Number of Cattle Antibody Tests 2019