Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - November 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 171
Report Period 1st - 30th November 2020
Issued 10 December 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
November
2020
%
Total 2020
%
Submissions
347
3188
Samples
Total (%)
26584
100.00 %
260301
100.00 %
England
19084
71.79 %
201915
77.57 %
Wales
6221
23.40 %
52577
20.20 %
Scotland
1279
4.81 %
5809
2.23 %
Parallel Tests
PAR-CUL-N
10680
40.17 %
95614
36.73 %
PAR-CUL-P
493
1.85 %
8597
3.30 %
PAR-HERD-S
10
0.04 %
1443
0.55 %
PAR-NEW-IN
8430
31.71 %
108585
41.72 %
PAR-OTHER
1237
4.65 %
7820
3.00 %
PAR-PERSIS
4878
18.35 %
32190
12.37 %
PAR-RAPID
169
0.64 %
1523
0.59 %
Total(% of all samples)
25897
97.42 %
255772
98.26 %
Serial/Extended Tests
SER-FLEXI
660
2.48 %
4483
1.72 %
SER-FRAUD
0
0.00 %
19
0.01 %
SER-NSR
27
0.10 %
27
0.01 %
Total(% of all samples)
687
2.58 %
4529
1.74 %
Total
26584
100.00 %
260301
100.00 %
Retests
Total (% of all samples)
1710
6.43 %
15796
6.07 %
England
1198
4.51 %
12328
4.74 %
Wales
449
1.69 %
3118
1.20 %
Scotland
63
0.24 %
350
0.13 %
Resamples
Total (% of all samples)
1426
5.36 %
12215
4.69 %
England
982
3.69 %
9442
3.63 %
Wales
391
1.47 %
2493
0.96 %
Scotland
53
0.20 %
280
0.11 %
Rejects
Total (% of all samples)
58
0.22 %
2404
0.92 %
England
24
0.09 %
2252
0.87 %
Wales
34
0.13 %
87
0.03 %
Scotland
0
0.00 %
65
0.02 %
Table 1. Monthly Totals for November 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CUL-N
10680
40.2 %
PAR-CUL-P
493
1.9 %
PAR-HERD-S
10
0.0 %
PAR-NEW-IN
8430
31.7 %
PAR-OTHER
1237
4.7 %
PAR-PERSIS
4878
18.3 %
PAR-RAPID
169
0.6 %
SER-FLEXI
660
2.5 %
SER-NSR
27
0.1 %
Total
26584
100.00 %
Figure 1. Number of samples submitted in each category during November 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Wigtown
Lanarkshire
Argyll & Bute
W Glamorgan
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during November 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
Wigtown
Perthshire
Lanarkshire
Kirkcudbright
Dumfries & G
Berwickshire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Kent
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
114
0.43 %
14
12.28 %
97
85.09 %
8
7.02 %
3
2.63 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
9
1,196
4.50 %
45
3.76 %
1036
86.62 %
138
11.54 %
114
9.53 %
1
0.08 %
Devon
PAR-CUL-N
48
5,552
20.88 %
274
4.94 %
4977
89.64 %
342
6.16 %
287
5.17 %
14
0.25 %
PAR-CUL-P
2
303
1.14 %
24
7.92 %
253
83.50 %
23
7.59 %
22
7.26 %
4
1.32 %
PAR-OTHER
1
15
0.06 %
9
60.00 %
6
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Dorset
PAR-CUL-N
10
994
3.74 %
115
11.57 %
819
82.39 %
71
7.14 %
60
6.04 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gloucs
PAR-CUL-N
11
1,176
4.42 %
72
6.12 %
1063
90.39 %
52
4.42 %
41
3.49 %
0
0.00 %
Heref
PAR-NEW-IN
1
33
0.12 %
1
3.03 %
30
90.91 %
4
12.12 %
2
6.06 %
0
0.00 %
Shropshire
PAR-CUL-N
3
42
0.16 %
0
0.00 %
32
76.19 %
10
23.81 %
9
21.43 %
1
2.38 %
PAR-PERSIS
5
1,401
5.27 %
57
4.07 %
1313
93.72 %
37
2.64 %
31
2.21 %
0
0.00 %
Somerset excl N
PAR-CUL-N
13
892
3.36 %
49
5.49 %
810
90.81 %
49
5.49 %
33
3.70 %
0
0.00 %
PAR-OTHER
1
391
1.47 %
66
xx-xxxx-xxxx %
311
79.54 %
16
4.09 %
14
3.58 %
0
0.00 %
Staffs
PAR-CUL-N
4
422
1.59 %
18
4.27 %
384
91.00 %
24
5.69 %
20
4.74 %
0
0.00 %
PAR-CUL-P
2
190
0.71 %
5
2.63 %
172
90.53 %
15
7.89 %
13
6.84 %
0
0.00 %
PAR-NEW-IN
3
703
2.64 %
47
6.69 %
628
89.33 %
30
4.27 %
27
3.84 %
1
0.14 %
Wiltshire
PAR-CUL-N
3
305
1.15 %
12
3.93 %
276
90.49 %
22
7.21 %
17
5.57 %
0
0.00 %
PAR-NEW-IN
1
124
0.47 %
1
0.81 %
121
97.58 %
4
3.23 %
2
1.61 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
1
142
0.53 %
3
2.11 %
129
90.85 %
14
9.86 %
10
7.04 %
0
0.00 %
Bucks
PAR-NEW-IN
1
47
0.18 %
1
2.13 %
29
61.70 %
16
34.04 %
16
34.04 %
1
2.13 %
Cheshire
PAR-CUL-N
1
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
14
2,474
9.31 %
50
2.02 %
2309
93.33 %
139
5.62 %
115
4.65 %
0
0.00 %
Derbyshire
PAR-NEW-IN
14
1,063
4.00 %
65
6.11 %
941
88.52 %
63
5.93 %
57
5.36 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
256
0.96 %
4
1.56 %
244
95.31 %
11
4.30 %
8
3.13 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
220
0.83 %
18
8.18 %
194
88.18 %
12
5.45 %
8
3.64 %
0
0.00 %
Northants
PAR-NEW-IN
6
508
1.91 %
14
2.76 %
452
88.98 %
56
11.02 %
40
7.87 %
2
0.39 %
Table 2a. Summary by County for November 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Oxon
PAR-NEW-IN
2
22
0.08 %
0
0.00 %
20
90.91 %
3
13.64 %
2
9.09 %
0
0.00 %
Warks
PAR-CUL-N
1
95
0.36 %
0
0.00 %
91
95.79 %
6
6.32 %
4
4.21 %
0
0.00 %
PAR-NEW-IN
4
375
1.41 %
12
3.20 %
340
90.67 %
29
7.73 %
23
6.13 %
0
0.00 %
PAR-PERSIS
1
17
0.06 %
0
0.00 %
14
82.35 %
3
17.65 %
3
17.65 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Lincs
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
169
19084
71.79 %
976
5.11 %
17102
89.61 %
1198
6.28 %
982
5.15 %
24
0.13 %
Wales - High TB
Carms
PAR-OTHER
8
317
1.19 %
28
8.83 %
280
88.33 %
11
3.47 %
9
2.84 %
0
0.00 %
PAR-PERSIS
9
550
2.07 %
32
5.82 %
489
88.91 %
34
6.18 %
29
5.27 %
0
0.00 %
PAR-RAPID
5
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
29
0.11 %
2
6.90 %
26
89.66 %
1
3.45 %
1
3.45 %
0
0.00 %
Ceredigion
PAR-OTHER
4
59
0.22 %
2
3.39 %
56
94.92 %
2
3.39 %
1
1.69 %
0
0.00 %
PAR-PERSIS
11
368
1.38 %
36
9.78 %
310
84.24 %
24
6.52 %
22
5.98 %
0
0.00 %
PAR-RAPID
5
12
0.05 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
440
1.66 %
39
8.86 %
378
85.91 %
29
6.59 %
23
5.23 %
0
0.00 %
Gwent
PAR-OTHER
1
7
0.03 %
1
14.29 %
5
71.43 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-PERSIS
2
13
0.05 %
3
23.08 %
10
76.92 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
4
0.02 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
10
396
1.49 %
25
6.31 %
351
88.64 %
23
5.81 %
18
4.55 %
2
0.51 %
PAR-PERSIS
25
1,684
6.33 %
56
3.33 %
1525
90.56 %
117
6.95 %
102
6.06 %
1
0.06 %
PAR-RAPID
15
86
0.32 %
10
11.63 %
67
77.91 %
10
11.63 %
9
10.47 %
0
0.00 %
SER-FLEXI
3
97
0.36 %
18
18.56 %
72
74.23 %
5
5.15 %
4
4.12 %
3
3.09 %
Powys
PAR-HERD-S
2
10
0.04 %
0
0.00 %
10
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
270
1.02 %
7
2.59 %
247
91.48 %
15
5.56 %
15
5.56 %
1
0.37 %
PAR-RAPID
16
52
0.20 %
5
9.62 %
45
86.54 %
1
1.92 %
1
1.92 %
1
1.92 %
SER-FLEXI
1
64
0.24 %
5
7.81 %
57
89.06 %
2
3.13 %
2
3.13 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
52
0.20 %
3
5.77 %
47
90.38 %
2
3.85 %
2
3.85 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for November 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
207
0.78 %
15
7.25 %
179
86.47 %
18
8.70 %
13
6.28 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
29
0.11 %
7
24.14 %
20
68.97 %
2
6.90 %
2
6.90 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
411
1.55 %
15
3.65 %
350
85.16 %
52
12.65 %
46
11.19 %
0
0.00 %
PAR-PERSIS
2
20
0.08 %
2
10.00 %
17
85.00 %
1
5.00 %
1
5.00 %
0
0.00 %
PAR-RAPID
3
7
0.03 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
2
74
0.28 %
1
1.35 %
65
87.84 %
8
10.81 %
8
10.81 %
0
0.00 %
S Glamorgan
PAR-PERSIS
2
128
0.48 %
20
15.63 %
93
72.66 %
16
12.50 %
15
11.72 %
0
0.00 %
W Glamorgan
PAR-PERSIS
4
106
0.40 %
7
6.60 %
94
88.68 %
4
3.77 %
3
2.83 %
2
1.89 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
31
0.12 %
0
0.00 %
31
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
9
689
2.59 %
13
1.89 %
588
85.34 %
69
10.01 %
64
9.29 %
24
3.48 %
Wales
169
6221
23.40 %
359
5.77 %
5437
87.40 %
449
7.22 %
391
6.29 %
34
0.55 %
Scotland - Scotland
Argyll & Bute
SER-NSR
2
27
0.10 %
4
14.81 %
21
77.78 %
2
7.41 %
2
7.41 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
3
782
2.94 %
8
1.02 %
736
94.12 %
47
6.01 %
38
4.86 %
0
0.00 %
Wigtown
PAR-NEW-IN
4
470
1.77 %
12
2.55 %
445
94.68 %
14
2.98 %
13
2.77 %
0
0.00 %
Scotland
9
1279
4.81 %
24
1.88 %
1202
93.98 %
63
4.93 %
53
4.14 %
0
0.00 %
Grand Total
Sum:
347
26584
1359
23741
1710
1426
58
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
114
0.04 %
14
12.28 %
97
85.09 %
8
7.02 %
3
2.63 %
0
0.00 %
SER-FLEXI
2
73
0.03 %
1
1.37 %
64
87.67 %
8
10.96 %
8
10.96 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
113
13,022
5.00 %
406
3.12 %
11832
90.86 %
926
7.11 %
683
5.24 %
101
0.78 %
PAR-CUL-P
7
1,802
0.69 %
46
2.55 %
xx-xxxx-xxxx
94.28 %
73
4.05 %
57
3.16 %
0
0.00 %
PAR-PERSIS
18
3,804
1.46 %
156
4.10 %
3516
92.43 %
186
4.89 %
131
3.44 %
1
0.03 %
SER-FLEXI
2
372
0.14 %
9
2.42 %
354
95.16 %
10
2.69 %
9
2.42 %
0
0.00 %
Devon
PAR-CUL-N
306
34,160
13.12 %
1783
5.22 %
30565
89.48 %
2100
6.15 %
1653
4.84 %
159
0.47 %
PAR-CUL-P
17
1,887
0.72 %
134
7.10 %
1637
86.75 %
135
7.15 %
110
5.83 %
6
0.32 %
PAR-NEW-IN
1
14
0.01 %
1
7.14 %
10
71.43 %
3
21.43 %
3
21.43 %
0
0.00 %
PAR-OTHER
1
15
0.01 %
9
60.00 %
6
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
92
0.04 %
8
8.70 %
80
86.96 %
8
8.70 %
4
4.35 %
0
0.00 %
SER-FLEXI
6
312
0.12 %
7
2.24 %
288
92.31 %
14
4.49 %
11
3.53 %
6
1.92 %
Dorset
PAR-CUL-N
72
8,745
3.36 %
549
6.28 %
7680
87.82 %
469
5.36 %
365
4.17 %
151
1.73 %
PAR-CUL-P
12
3,059
1.18 %
264
8.63 %
2661
86.99 %
183
5.98 %
133
4.35 %
1
0.03 %
PAR-NEW-IN
2
167
0.06 %
5
2.99 %
156
93.41 %
11
6.59 %
6
3.59 %
0
0.00 %
PAR-PERSIS
2
19
0.01 %
0
0.00 %
16
84.21 %
3
15.79 %
3
15.79 %
0
0.00 %
SER-FLEXI
10
808
0.31 %
52
6.44 %
735
90.97 %
34
4.21 %
21
2.60 %
0
0.00 %
Gloucs
PAR-CUL-N
67
6,107
2.35 %
286
4.68 %
5426
88.85 %
501
8.20 %
391
6.40 %
4
0.07 %
PAR-NEW-IN
1
38
0.01 %
4
10.53 %
32
84.21 %
4
10.53 %
2
5.26 %
0
0.00 %
PAR-OTHER
2
63
0.02 %
1
1.59 %
55
87.30 %
8
12.70 %
7
11.11 %
0
0.00 %
PAR-PERSIS
2
242
0.09 %
14
5.79 %
220
90.91 %
10
4.13 %
8
3.31 %
0
0.00 %
Heref
PAR-CUL-N
18
2,820
1.08 %
102
3.62 %
2614
92.70 %
137
4.86 %
99
3.51 %
5
0.18 %
PAR-NEW-IN
10
687
0.26 %
24
3.49 %
616
89.67 %
63
9.17 %
46
6.70 %
1
0.15 %
PAR-OTHER
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
213
0.08 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Shropshire
PAR-CUL-N
25
5,539
2.13 %
111
2.00 %
5193
93.75 %
325
5.87 %
232
4.19 %
3
0.05 %
PAR-PERSIS
12
2,416
0.93 %
117
4.84 %
2212
91.56 %
102
4.22 %
85
3.52 %
2
0.08 %
SER-FLEXI
3
389
0.15 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
121
12,244
4.70 %
486
3.97 %
11325
92.49 %
600
4.90 %
404
3.30 %
29
0.24 %
PAR-CUL-P
3
418
0.16 %
13
3.11 %
391
93.54 %
18
4.31 %
14
3.35 %
0
0.00 %
PAR-OTHER
1
391
0.15 %
66
xx-xxxx-xxxx %
311
79.54 %
16
4.09 %
14
3.58 %
0
0.00 %
PAR-PERSIS
3
293
0.11 %
25
8.53 %
258
88.05 %
14
4.78 %
10
3.41 %
0
0.00 %
SER-FLEXI
5
202
0.08 %
7
3.47 %
191
94.55 %
4
1.98 %
4
1.98 %
0
0.00 %
Staffs
PAR-CUL-N
25
3,322
1.28 %
96
2.89 %
2911
87.63 %
181
5.45 %
159
4.79 %
156
4.70 %
PAR-CUL-P
2
190
0.07 %
5
2.63 %
172
90.53 %
15
7.89 %
13
6.84 %
0
0.00 %
PAR-NEW-IN
54
7,500
2.88 %
367
4.89 %
6857
91.43 %
352
4.69 %
274
3.65 %
2
0.03 %
Wiltshire
PAR-CUL-N
69
8,323
3.20 %
229
2.75 %
7647
91.88 %
566
6.80 %
427
5.13 %
20
0.24 %
PAR-CUL-P
7
1,241
0.48 %
69
5.56 %
1096
88.32 %
95
7.66 %
65
5.24 %
11
0.89 %
PAR-NEW-IN
2
125
0.05 %
1
0.80 %
122
97.60 %
4
3.20 %
2
1.60 %
0
0.00 %
PAR-PERSIS
2
104
0.04 %
5
4.81 %
93
89.42 %
7
6.73 %
6
5.77 %
0
0.00 %
SER-FLEXI
2
45
0.02 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
25
2,783
1.07 %
78
2.80 %
2498
89.76 %
269
9.67 %
207
7.44 %
0
0.00 %
PAR-PERSIS
3
319
0.12 %
2
0.63 %
308
96.55 %
11
3.45 %
9
2.82 %
0
0.00 %
Bucks
PAR-NEW-IN
16
2,010
0.77 %
80
3.98 %
1753
87.21 %
212
10.55 %
176
8.76 %
1
0.05 %
Cheshire
PAR-CUL-N
7
874
0.34 %
14
1.60 %
843
96.45 %
28
3.20 %
17
1.95 %
0
0.00 %
PAR-NEW-IN
155
26,862
10.32 %
540
2.01 %
25171
93.70 %
1422
5.29 %
1101
4.10 %
50
0.19 %
Derbyshire
PAR-NEW-IN
135
15,617
6.00 %
557
3.57 %
13401
85.81 %
1112
7.12 %
907
5.81 %
752
4.82 %
E Sussex
PAR-NEW-IN
28
2,226
0.86 %
67
3.01 %
2064
92.72 %
112
5.03 %
95
4.27 %
0
0.00 %
Hampshire
PAR-CUL-N
2
363
0.14 %
15
4.13 %
327
90.08 %
39
10.74 %
19
5.23 %
2
0.55 %
PAR-NEW-IN
34
3,991
1.53 %
117
2.93 %
3681
92.23 %
278
6.97 %
193
4.84 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
48
4,740
1.82 %
179
3.78 %
4316
91.05 %
307
6.48 %
227
4.79 %
18
0.38 %
Northants
PAR-NEW-IN
26
2,091
0.80 %
72
3.44 %
1907
91.20 %
136
6.50 %
109
5.21 %
3
0.14 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Notts
PAR-NEW-IN
6
366
0.14 %
4
1.09 %
350
95.63 %
17
4.64 %
11
3.01 %
1
0.27 %
Oxon
PAR-NEW-IN
84
7,811
3.00 %
345
4.42 %
6821
87.33 %
494
6.32 %
357
4.57 %
288
3.69 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Warks
PAR-CUL-N
1
95
0.04 %
0
0.00 %
91
95.79 %
6
6.32 %
4
4.21 %
0
0.00 %
PAR-NEW-IN
68
6,024
2.31 %
165
2.74 %
5477
90.92 %
371
6.16 %
278
4.61 %
104
1.73 %
PAR-PERSIS
3
496
0.19 %
20
4.03 %
460
92.74 %
23
4.64 %
16
3.23 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
384
0.15 %
4
1.04 %
363
94.53 %
32
8.33 %
17
4.43 %
0
0.00 %
Cleveland
PAR-NEW-IN
1
50
0.02 %
2
4.00 %
48
96.00 %
1
2.00 %
0
0.00 %
0
0.00 %
Cumbria
PAR-NEW-IN
4
51
0.02 %
1
1.96 %
42
82.35 %
9
17.65 %
8
15.69 %
0
0.00 %
PAR-PERSIS
1
33
0.01 %
1
3.03 %
28
84.85 %
4
12.12 %
4
12.12 %
0
0.00 %
Essex
PAR-NEW-IN
3
265
0.10 %
3
1.13 %
252
95.09 %
11
4.15 %
10
3.77 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
7
336
0.13 %
6
1.79 %
114
33.93 %
6
1.79 %
6
1.79 %
210
62.50 %
Humberside
PAR-NEW-IN
2
78
0.03 %
7
8.97 %
66
84.62 %
7
8.97 %
5
6.41 %
0
0.00 %
Kent
PAR-NEW-IN
1
58
0.02 %
1
1.72 %
53
91.38 %
4
6.90 %
4
6.90 %
0
0.00 %
Lincs
PAR-NEW-IN
8
360
0.14 %
15
4.17 %
327
90.83 %
19
5.28 %
18
5.00 %
0
0.00 %
PAR-PERSIS
2
34
0.01 %
0
0.00 %
31
91.18 %
5
14.71 %
3
8.82 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
49
0.02 %
1
2.04 %
29
59.18 %
6
12.24 %
6
12.24 %
13
26.53 %
Northumb
PAR-NEW-IN
2
307
0.12 %
17
5.54 %
240
78.18 %
40
13.03 %
50
16.29 %
0
0.00 %
N Yorks
PAR-NEW-IN
10
849
0.33 %
16
1.88 %
713
83.98 %
40
4.71 %
29
3.42 %
91
10.72 %
Surrey
PAR-NEW-IN
3
429
0.16 %
17
3.96 %
396
92.31 %
18
4.20 %
16
3.73 %
0
0.00 %
W Yorks
PAR-NEW-IN
8
563
0.22 %
4
0.71 %
473
84.01 %
37
6.57 %
25
4.44 %
61
10.83 %
England
1715
201915
77.57 %
7851
3.89 %
182370
90.32 %
12328
6.11 %
9442
4.68 %
2252
1.12 %
Wales - High TB
Carms
PAR-HERD-S
12
678
0.26 %
51
7.52 %
598
88.20 %
45
6.64 %
29
4.28 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
50
1,126
0.43 %
151
13.41 %
929
82.50 %
55
4.88 %
46
4.09 %
0
0.00 %
PAR-PERSIS
65
3,602
1.38 %
262
7.27 %
3175
88.15 %
202
5.61 %
160
4.44 %
5
0.14 %
PAR-RAPID
78
288
0.11 %
54
18.75 %
216
75.00 %
26
9.03 %
18
6.25 %
0
0.00 %
SER-FLEXI
9
113
0.04 %
24
21.24 %
85
75.22 %
3
2.65 %
2
1.77 %
2
1.77 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-HERD-S
2
13
0.00 %
0
0.00 %
7
53.85 %
6
46.15 %
6
46.15 %
0
0.00 %
PAR-OTHER
36
751
0.29 %
68
9.05 %
652
86.82 %
41
5.46 %
31
4.13 %
0
0.00 %
PAR-PERSIS
40
1,824
0.70 %
105
5.76 %
1620
88.82 %
108
5.92 %
97
5.32 %
2
0.11 %
PAR-RAPID
42
125
0.05 %
34
27.20 %
86
68.80 %
7
5.60 %
5
4.00 %
0
0.00 %
SER-FLEXI
4
480
0.18 %
39
8.13 %
416
86.67 %
31
6.46 %
25
5.21 %
0
0.00 %
Gwent
PAR-HERD-S
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
8
0.00 %
2
25.00 %
5
62.50 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-PERSIS
27
1,460
0.56 %
95
6.51 %
1321
90.48 %
68
4.66 %
43
2.95 %
1
0.07 %
PAR-RAPID
21
114
0.04 %
20
17.54 %
91
79.82 %
5
4.39 %
3
2.63 %
0
0.00 %
SER-FLEXI
9
284
0.11 %
27
9.51 %
251
88.38 %
10
3.52 %
6
2.11 %
0
0.00 %
Pembs
PAR-HERD-S
7
292
0.11 %
11
3.77 %
271
92.81 %
12
4.11 %
10
3.42 %
0
0.00 %
PAR-NEW-IN
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
120
2,889
1.11 %
293
10.14 %
2487
86.09 %
140
4.85 %
104
3.60 %
5
0.17 %
PAR-PERSIS
176
11,508
4.42 %
528
4.59 %
10471
90.99 %
610
5.30 %
497
4.32 %
12
0.10 %
PAR-RAPID
141
615
0.24 %
123
20.00 %
465
75.61 %
33
5.37 %
26
4.23 %
1
0.16 %
SER-FLEXI
25
564
0.22 %
35
6.21 %
508
90.07 %
29
5.14 %
18
3.19 %
3
0.53 %
Powys
PAR-HERD-S
17
272
0.10 %
9
3.31 %
263
96.69 %
3
1.10 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
116
0.04 %
6
5.17 %
108
93.10 %
2
1.72 %
2
1.72 %
0
0.00 %
PAR-OTHER
17
994
0.38 %
52
5.23 %
858
86.32 %
92
9.26 %
83
8.35 %
1
0.10 %
PAR-PERSIS
62
2,218
0.85 %
118
5.32 %
1924
86.74 %
193
8.70 %
166
7.48 %
10
0.45 %
PAR-RAPID
61
193
0.07 %
27
13.99 %
161
83.42 %
5
2.59 %
4
2.07 %
1
0.52 %
SER-FLEXI
11
276
0.11 %
22
7.97 %
243
88.04 %
21
7.61 %
11
3.99 %
0
0.00 %
S Wales
PAR-PERSIS
2
274
0.11 %
12
4.38 %
260
94.89 %
8
2.92 %
2
0.73 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
36
0.01 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-PERSIS
5
18
0.01 %
0
0.00 %
16
88.89 %
4
22.22 %
2
11.11 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
30
0.01 %
5
16.67 %
24
80.00 %
3
10.00 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
1
155
0.06 %
34
21.94 %
119
76.77 %
2
1.29 %
2
1.29 %
0
0.00 %
PAR-OTHER
12
283
0.11 %
23
8.13 %
248
87.63 %
14
4.95 %
12
4.24 %
0
0.00 %
PAR-PERSIS
14
784
0.30 %
49
6.25 %
705
89.92 %
38
4.85 %
30
3.83 %
0
0.00 %
PAR-RAPID
8
12
0.00 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
SER-FLEXI
2
29
0.01 %
7
24.14 %
20
68.97 %
2
6.90 %
2
6.90 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
171
0.07 %
12
7.02 %
152
88.89 %
8
4.68 %
7
4.09 %
0
0.00 %
PAR-RAPID
5
29
0.01 %
8
27.59 %
21
72.41 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
48
0.02 %
6
12.50 %
40
83.33 %
2
4.17 %
2
4.17 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
93
8,738
3.36 %
243
2.78 %
8020
91.78 %
568
6.50 %
472
5.40 %
3
0.03 %
PAR-OTHER
26
385
0.15 %
31
8.05 %
335
87.01 %
23
5.97 %
19
4.94 %
0
0.00 %
PAR-PERSIS
20
1,572
0.60 %
92
5.85 %
1427
90.78 %
64
4.07 %
50
3.18 %
3
0.19 %
PAR-RAPID
32
93
0.04 %
21
22.58 %
69
74.19 %
3
3.23 %
3
3.23 %
0
0.00 %
SER-FLEXI
11
430
0.17 %
21
4.88 %
380
88.37 %
37
8.60 %
27
6.28 %
2
0.47 %
Powys
PAR-NEW-IN
19
2,096
0.81 %
43
2.05 %
1946
92.84 %
122
5.82 %
103
4.91 %
4
0.19 %
PAR-OTHER
4
103
0.04 %
1
0.97 %
89
86.41 %
18
17.48 %
13
12.62 %
0
0.00 %
PAR-PERSIS
4
283
0.11 %
4
1.41 %
263
92.93 %
19
6.71 %
16
5.65 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-PERSIS
2
128
0.05 %
20
15.63 %
93
72.66 %
16
12.50 %
15
11.72 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
13
0.00 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-PERSIS
4
106
0.04 %
7
6.60 %
94
88.68 %
4
3.77 %
3
2.83 %
2
1.89 %
PAR-RAPID
1
7
0.00 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
153
0.06 %
1
0.65 %
146
95.42 %
7
4.58 %
6
3.92 %
0
0.00 %
PAR-NEW-IN
34
1,770
0.68 %
33
1.86 %
1660
93.79 %
101
5.71 %
74
4.18 %
3
0.17 %
PAR-OTHER
3
9
0.00 %
0
0.00 %
5
55.56 %
4
44.44 %
4
44.44 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
2
12
0.00 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
NE Wales
PAR-HERD-S
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-NEW-IN
35
3,896
1.50 %
74
1.90 %
3578
91.84 %
283
7.26 %
217
5.57 %
27
0.69 %
PAR-OTHER
3
14
0.01 %
1
7.14 %
11
78.57 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-PERSIS
1
40
0.02 %
0
0.00 %
32
80.00 %
10
25.00 %
8
20.00 %
0
0.00 %
PAR-RAPID
4
21
0.01 %
1
4.76 %
20
95.24 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
1415
52577
20.20 %
2924
5.56 %
47073
89.53 %
3118
5.93 %
2493
4.74 %
87
0.17 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
7
588
0.23 %
32
5.44 %
455
77.38 %
41
6.97 %
36
6.12 %
65
11.05 %
PAR-OTHER
7
782
0.30 %
15
1.92 %
700
89.51 %
94
12.02 %
67
8.57 %
0
0.00 %
SER-NSR
2
27
0.01 %
4
14.81 %
21
77.78 %
2
7.41 %
2
7.41 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
383
0.15 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Berwickshire
PAR-NEW-IN
2
78
0.03 %
8
10.26 %
62
79.49 %
8
10.26 %
8
10.26 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
6
849
0.33 %
12
1.41 %
802
94.46 %
38
4.48 %
35
4.12 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
15
807
0.31 %
22
2.73 %
762
94.42 %
34
4.21 %
23
2.85 %
0
0.00 %
PAR-PERSIS
1
22
0.01 %
0
0.00 %
22
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
3
782
0.30 %
8
1.02 %
736
94.12 %
47
6.01 %
38
4.86 %
0
0.00 %
Perthshire
PAR-NEW-IN
2
357
0.14 %
0
0.00 %
345
96.64 %
14
3.92 %
12
3.36 %
0
0.00 %
Wigtown
PAR-NEW-IN
10
1,134
0.44 %
29
2.56 %
1069
94.27 %
42
3.70 %
36
3.17 %
0
0.00 %
Scotland
58
5809
2.23 %
131
2.26 %
5333
91.81 %
350
6.03 %
280
4.82 %
65
1.12 %
Grand Total
Sum:
xx-xxxx-xxxx
10906
234776
15796
12215
2404
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol November 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
168
19083
100.0%
976
5.1%
17101
89.6%
1198
6.3%
982
5.1%
24
0.1%
Serial/Extended
1
1
0.0%
0
0.0 %
1 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
169
19084
100.0%
976
5.1%
17102
89.6%
1198
6.3%
982
5.1%
24
0.1%
Scotland
Parallel
7
1252
97.9%
20
1.6%
1181
94.3%
61
4.9%
51
4.1%
0
0.0 %
Serial/Extended
2
27
2.1%
4
0.3%
21
77.8%
2
7.4%
2
7.4%
0
0.0 %
Total
9
1279
100.0%
24
1.9%
1202
94.0%
63
4.9%
53
4.1%
0
0.0 %
Wales
Parallel
158
5562
89.4%
288
5.2%
4884
87.8%
410
7.4%
359
6.5%
31
0.6%
Serial/Extended
11
659
10.6%
71
10.8%
553
83.9%
39
5.9%
32
4.9%
3
0.5%
Total
169
6221
100.0%
359
5.8%
5437
87.4%
449
7.2%
391
6.3%
34
0.5%
GB
Parallel
333
25897
97.4%
1284
5.0%
23166
89.5%
1669
6.4%
1392
5.4%
55
0.2%
Serial/Extended
14
687
2.6%
75
10.9%
575
83.7%
41
6.0%
34
4.9%
3
0.4%
Total
347
26584
100.0%
1359
5.1%
23741
89.3%
1710
6.4%
1426
5.4%
58
0.2%
xx-xxxx-xxxx
xx-xxxx-xxxx
9.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx 199695
98.9%
7755
3.9% 180326
90.3%
12231
6.1%
9368
4.7%
2246
1.1%
Serial/Extended
32
2220
1.1%
96
4.3%
2044
92.1%
97
4.4%
74
3.3%
6
0.3%
Total
xx-xxxx-xxxx
100.0%
7851
3.9% 182370
90.3%
12328
6.1%
9442
4.7%
2252
1.1%
Scotland
Parallel
56
5782
99.5%
127
2.2%
5312
91.9%
348
6.0%
278
4.8%
65
1.1%
Serial/Extended
2
27
0.5%
4
0.1%
21
77.8%
2
7.4%
2
7.4%
0
0.0 %
Total
58
5809
100.0%
131
2.3%
5333
91.8%
350
6.0%
280
4.8%
65
1.1%
Wales
Parallel
1337
50295
95.7%
2737
5.4%
45078
89.6%
2983
5.9%
2400
4.8%
80
0.2%
Serial/Extended
78
2282
4.3%
187
8.2%
1995
87.4%
135
5.9%
93
4.1%
7
0.3%
Total
1415
52577
100.0%
2924
5.6%
47073
89.5%
3118
5.9%
2493
4.7%
87
0.2%
GB
Parallel
xx-xxxx-xxxx
98.3% 10619
4.2% 230716
90.2%
15562
6.1%
12046
4.7%
2391
0.9%
Serial/Extended
112
4529
1.7%
287
6.3%
4060
89.6%
234
5.2%
169
3.7%
13
0.3%
Total
xx-xxxx-xxxx
100.0% 10906
4.2% 234776
90.2%
15796
6.1%
12215
4.7%
2404
0.9%
#######
#######
58.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
12
660
2.5%
71
10.8%
554
83.9%
39
5.9 %
32
4.8 %
3
0.5 %
Parallel - Herd or Group Slaughter
2
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Parallel New Infection
80
8430
31.7%
265
3.1%
7657
90.8%
572
6.8 %
480
5.7 %
28
0.3 %
Parallel - new infection cull area
103
10680
40.2%
585
5.5%
9494
88.9%
714
6.7 %
585
5.5 %
16
0.1 %
Parallel - Other
26
1237
4.7%
134
10.8%
1056
85.4%
55
4.4 %
45
3.6 %
2
0.2 %
Parallel Persistent Infection
70
4878
18.3%
249
5.1%
4388
90.0%
277
5.7 %
237
4.9 %
4
0.1 %
Parallel - persistent infection cull area
4
493
1.9%
29
5.9%
425
86.2%
38
7.7 %
35
7.1 %
4
0.8 %
Parallel - Rapid Testing Two-times IRs
48
169
0.6%
22
13.0%
136
80.5%
13
7.7 %
10
5.9 %
1
0.6 %
Serial - Suspected NSR Herd
2
27
0.1%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Total
347
26584
100.0%
1359
5.1%
23741
89.3%
1710
6.4 %
1426
5.4 %
58
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (November 2020)
England - HRA
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
5
860
3.2%
49
5.7%
779
90.6%
38
4.4 %
31
3.6 %
1
0.1 %
Parallel - new infection cull area
101
10579
39.8%
585
5.5%
9397
88.8%
708
6.7 %
581
5.5 %
16
0.2 %
Parallel - Other
2
406
1.5%
75
18.5%
317
78.1%
16
3.9 %
14
3.4 %
0
Parallel Persistent Infection
6
1515
5.7%
71
4.7%
1410
93.1%
45
3.0 %
34
2.2 %
0
Parallel - persistent infection cull area
4
493
1.9%
29
5.9%
425
86.2%
38
7.7 %
35
7.1 %
4
0.8 %
England - HRA
119
13854
52.1%
809
5.8%
12329
89.0%
845
6.1 %
695
5.0 %
21
0.2 %
England - Edge
Parallel New Infection
45
5107
19.2%
167
3.3%
4658
91.2%
343
6.7 %
279
5.5 %
3
0.1 %
Parallel - new infection cull area
2
101
0.4%
0
0.0 %
97
96.0%
6
5.9 %
4
4.0 %
0
Parallel Persistent Infection
1
17
0.1%
0
0.0 %
14
82.4%
3
17.6 %
3
17.6 %
0
England - Edge
48
5225
19.7%
167
3.2%
4769
91.3%
352
6.7 %
286
5.5 %
3
0.1 %
England - LRA
Parallel New Infection
2
5
0.0%
0
0.0 %
4
80.0%
1
20.0 %
1
20.0 %
0
England - LRA
2
5
0.0%
0
0.0 %
4
80.0%
1
20.0 %
1
20.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
9
630
2.4%
64
10.2%
533
84.6%
37
5.9 %
30
4.8 %
3
0.5 %
Parallel - Herd or Group Slaughter
2
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Parallel New Infection
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Other
23
779
2.9%
56
7.2%
692
88.8%
37
4.7 %
29
3.7 %
2
0.3 %
Parallel Persistent Infection
53
2885
10.9%
134
4.6%
2581
89.5%
190
6.6 %
168
5.8 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
44
161
0.6%
20
12.4%
130
80.7%
13
8.1 %
10
6.2 %
1
0.6 %
Wales - High TB
132
4466
16.8%
274
6.1%
3947
88.4%
277
6.2 %
237
5.3 %
8
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
29
0.1%
7
24.1%
20
69.0%
2
6.9 %
2
6.9 %
0
Parallel New Infection
9
485
1.8%
16
3.3%
415
85.6%
60
12.4 %
54
11.1 %
0
Parallel - Other
1
52
0.2%
3
5.8%
47
90.4%
2
3.8 %
2
3.8 %
0
Parallel Persistent Infection
10
461
1.7%
44
9.5%
383
83.1%
39
8.5 %
32
6.9 %
2
0.4 %
Parallel - Rapid Testing Two-times IRs
4
8
0.0%
2
25.0%
6
75.0%
0
0
0
Wales - Intermediate TB
26
1035
3.9%
72
7.0%
871
84.2%
103
10.0 %
90
8.7 %
2
0.2 %
Wales - Low TB
Parallel New Infection
11
720
2.7%
13
1.8%
619
86.0%
69
9.6 %
64
8.9 %
24
3.3 %
Wales - Low TB
11
720
2.7%
13
1.8%
619
86.0%
69
9.6 %
64
8.9 %
24
3.3 %
Scotland - Scotland
Parallel New Infection
7
1252
4.7%
20
1.6%
1181
94.3%
61
4.9 %
51
4.1 %
0
Serial - Suspected NSR Herd
2
27
0.1%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Scotland - Scotland
9
1279
4.8%
24
1.9%
1202
94.0%
63
4.9 %
53
4.1 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
108
4483
1.7%
283
6.3%
4020
89.7%
232
5.2 %
167
3.7 %
13
0.3 %
Parallel - Herd or Group Slaughter
46
1443
0.6%
78
5.4%
1312
90.9%
77
5.3 %
53
3.7 %
0
Parallel New Infection
986 108585
41.7%
3245
3.0%
98374
90.6%
6729
6.2 %
5269
4.9 %
xx-xxxx-xxxx
1.6 %
Parallel - new infection cull area
826
95614
36.7%
4077
4.3%
86454
90.4%
5878
6.1 %
4453
4.7 %
630
0.7 %
Parallel - Other
286
7820
3.0%
713
9.1%
6695
85.6%
511
6.5 %
406
5.2 %
6
0.1 %
Parallel Persistent Infection
480
32190
12.4%
1675
5.2%
29067
90.3%
1765
5.5 %
1410
4.4 %
38
0.1 %
Parallel - persistent infection cull area
48
8597
3.3%
531
6.2%
7656
89.1%
519
6.0 %
392
4.6 %
18
0.2 %
Parallel - Rapid Testing Two-times IRs
404
1523
0.6%
300
19.7%
1158
76.0%
83
5.4 %
63
4.1 %
2
0.1 %
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
Serial - Suspected NSR Herd
2
27
0.0%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Total
xx-xxxx-xxxx
100.0%
10906
4.2%
234776
90.2%
15796
6.1 %
12215
4.7 %
2404
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
30
2201
0.8%
96
4.4%
2025
92.0%
97
4.4 %
74
3.4 %
6
0.3 %
Parallel New Infection
70
8531
3.3%
402
4.7%
7793
91.3%
437
5.1 %
333
3.9 %
3
0.0 %
Parallel - new infection cull area
816
94282
36.2%
4048
4.3%
85193
90.4%
5805
6.2 %
4413
4.7 %
628
0.7 %
Parallel - Other
5
473
0.2%
76
16.1%
376
79.5%
24
5.1 %
21
4.4 %
0
Parallel Persistent Infection
43
7297
2.8%
348
4.8%
6664
91.3%
370
5.1 %
282
3.9 %
3
0.0 %
Parallel - persistent infection cull area
48
8597
3.3%
531
6.2%
7656
89.1%
519
6.0 %
392
4.6 %
18
0.2 %
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
England - Edge
Parallel New Infection
625
74521
28.6%
2204
3.0%
67439
90.5%
4730
6.3 %
3661
4.9 %
1217
1.6 %
Parallel - new infection cull area
10
1332
0.5%
29
2.2%
1261
94.7%
73
5.5 %
40
3.0 %
2
0.2 %
Parallel Persistent Infection
7
816
0.3%
22
2.7%
769
94.2%
34
4.2 %
25
3.1 %
0
England - LRA
Parallel New Infection
56
3779
1.5%
94
2.5%
3116
82.5%
230
6.1 %
194
5.1 %
375
9.9 %
Parallel Persistent Infection
3
67
0.0%
1
1.5%
59
88.1%
9
13.4 %
7
10.4 %
0
England
xx-xxxx-xxxx
77.6%
7851
3.9%
182370
90.3%
12328
6.1 %
9442
4.7 %
2252
1.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
60
1762
0.7%
148
8.4%
1547
87.8%
94
5.3 %
62
3.5 %
5
0.3 %
Parallel - Herd or Group Slaughter
39
1258
0.5%
72
5.7%
1141
90.7%
66
5.2 %
45
3.6 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2020) Cont.
Parallel New Infection
5
121
0.0%
6
5.0%
113
93.4%
2
1.7 %
2
1.7 %
0
Parallel - Other
226
5771
2.2%
566
9.8%
4931
85.4%
332
5.8 %
268
4.6 %
6
0.1 %
Parallel Persistent Infection
377
20904
8.0%
1120
5.4%
18787
89.9%
1193
5.7 %
967
4.6 %
30
0.1 %
Parallel - Rapid Testing Two-times IRs
347
1343
0.5%
261
19.4%
1024
76.2%
76
5.7 %
56
4.2 %
2
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
18
520
0.2%
39
7.5%
448
86.2%
41
7.9 %
31
6.0 %
2
0.4 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
113
10989
4.2%
320
2.9%
10085
91.8%
692
6.3 %
577
5.3 %
7
0.1 %
Parallel - Other
42
771
0.3%
55
7.1%
672
87.2%
55
7.1 %
44
5.7 %
0
Parallel Persistent Infection
48
3044
1.2%
184
6.0%
2734
89.8%
149
4.9 %
121
4.0 %
5
0.2 %
Parallel - Rapid Testing Two-times IRs
51
147
0.1%
38
25.9%
105
71.4%
4
2.7 %
4
2.7 %
0
Wales - Low TB
Parallel - Herd or Group Slaughter
4
155
0.1%
1
0.6%
147
94.8%
8
5.2 %
7
4.5 %
0
Parallel New Infection
69
5666
2.2%
107
1.9%
5238
92.4%
384
6.8 %
291
5.1 %
30
0.5 %
Parallel - Other
6
23
0.0%
1
4.3%
16
69.6%
6
26.1 %
6
26.1 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
6
33
0.0%
1
3.0%
29
87.9%
3
9.1 %
3
9.1 %
0
Wales
1415
52577
20.2%
2924
5.6%
47073
89.5%
3118
5.9 %
2493
4.7 %
87
0.2 %
Scotland - Scotland
Parallel New Infection
48
4978
1.9%
112
2.2%
4590
92.2%
254
5.1 %
211
4.2 %
65
1.3 %
Parallel - Other
7
782
0.3%
15
1.9%
700
89.5%
94
12.0 %
67
8.6 %
0
Parallel Persistent Infection
1
22
0.0%
0
0.0 %
22
100.0%
0
0
0
Serial - Suspected NSR Herd
2
27
0.0%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Scotland
58
5809
2.2%
131
2.3%
5333
91.8%
350
6.0 %
280
4.8 %
65
1.1 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 December
234
25192
138
904
2
0
0
0
0
0
87
2
0
4
0
759
0
49
0
1
2020 January
343
31274
227
1367
1
7
0
0
0
0
178
3
0
8
0
1110
1
57
0
0
February
323
33146
192
1289
1
21
0
0
0
0
187
3
0
6
0
982
0
89
0
0
March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
1
70
0
0
August
290
24676
173
888
15
0
0
0
0
145
0
0
3
1
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
0
2
3
1
617
39
26
0
1
October
316
26668
189
1198
28
0
0
0
0
112
0
8
0
0
977
72
0
0
0
November
347
26584
200
1359
343
758
0
0
0
0
12
0
0
0
0
92
19
0
0
0
Totals (last
12 months)
3422
285493
1993
11810
351
887
0
0
0
0
1266
14
10
47
2
8398
133
560
0
3
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1858
221510
1176
8474
674
0
0
0
0
1143
7
4
17
2
6231
6
44
0
0
Scotland
58
5809
30
131
5
0
0
0
0
13
0
0
5
0
71
4
17
0
0
Wales
1506
58174
787
3205
208
0
0
0
0
110
7
6
25
0
2096
123
499
0
3
Totals
3422
285493
1993
11810
887
0
0
0
0
1266
14
10
47
2
8398
133
560
0
3
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
W Glamorgan
Staffs
Shropshire
Powys
Oxon
NE Wales
Lanarkshire
Hampshire
Gwent
E Sussex
Devon
Cumbria
Cheshire
Carms
Berks
Argyll & Bute
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during November 2020 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
7.41 %
7.41 %
Avon
2.63 %
7.02 %
Berks
7.04 %
9.86 %
Bucks
34.04 %
34.04 %
Carms
4.70 %
5.70 %
Ceredigion
5.23 %
6.26 %
Cheshire
4.64 %
5.60 %
Corn & Scilly
9.53 %
11.54 %
Cumbria
33.33 %
33.33 %
Derbyshire
5.36 %
5.93 %
Devon
5.26 %
6.22 %
Dorset
6.03 %
7.14 %
E Sussex
3.13 %
4.30 %
Gloucs
3.49 %
4.42 %
Gwent
4.17 %
12.50 %
Gwynedd
Hampshire
3.64 %
5.45 %
Heref
6.06 %
12.12 %
Lanarkshire
4.86 %
6.01 %
Lincs
NE Wales
9.85 %
10.83 %
Northants
7.87 %
11.02 %
Oxon
9.09 %
13.64 %
Pembs
5.88 %
6.85 %
Powys
5.52 %
5.52 %
S Glamorgan
11.72 %
12.50 %
Shropshire
2.77 %
3.26 %
Somerset excl
3.66 %
5.07 %
Staffs
4.56 %
5.25 %
Warks
6.16 %
7.80 %
W Glamorgan
2.83 %
3.77 %
Wigtown
2.77 %
2.98 %
Wiltshire
4.43 %
6.06 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18784
976
5.2%
19084
976
5.1%
Wales
6015
359
6.0%
6221
359
5.8%
Scotland
1270
24
1.9%
1279
24
1.9%
GB
26069
1359
5.2%
26584
1359
5.1%
Table 6a (i): Number of animals and submissions tested in November 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
191568
7851
4.1%
201915
7851
3.9%
Wales
48393
2923
6.0%
52577
2924
5.6%
Scotland
4905
131
2.7%
5809
131
2.3%
GB
244864
10905
4.5%
260301
10906
4.2%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22854
771
3.4%
23070
771
3.3%
Wales
6848
309
4.5%
7111
309
4.3%
Scotland
5
0
0.0 %
5
0.0 %
GB
29707
1080
3.6%
30186
1080
3.6%
Table 6a (ii): Number of animals and submissions tested in November 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of November
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
215085
9403
4.4%
244179
9403
3.9%
Wales
54800
2917
5.3%
59902
2917
4.9%
Scotland
2015
90
4.5%
2693
90
3.3%
GB
271893
12410
4.6%
306774
12410
4.0%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
2
170
8
4.71 %
Total
2
170
8
4.71 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples November 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SE
1
12
1
8.33 %
PRV-SP
3
3
0
0
Cumbria
PRV-SP
1
1
0
0
Devon
PRV-SE
3
289
8
2.77 %
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
3
5
0
0
East Sussex
PRV-SP
1
1
0
0
Essex
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Hampshire
PRV-SP
1
3
0
0
Northern Somerset & South Glou
PRV-SE
1
39
1
2.56 %
Oxfordshire
PRV-SP
1
32
0
0
Somerset excl North
PRV-SE
1
28
0
0
PRV-SP
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Wiltshire
PRV-SE
2
280
15
5.36 %
Worcestershire
PRV-SP
1
1
0
0
Total
26
739
28
3.79 %
Isle of Man
SER-IOM
19
186
0
0
Isle of Man
SER-IOM
4
13
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
7
81
18
22.2%
Ceredigion
9
283
36
12.7%
Cornwall and Isles of
Scilly
1
85
3
3.5%
North East Wales
2
25
4
16.0%
Pembrokeshire
17
142
22
15.5%
Powys
12
156
8
5.1%
South Wales
3
8
1
12.5%
TC0077
51
780
92
11.8 %
Table 8a Number of Cattle Antibody Tests November 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Berkshire
2
315
11
3.5%
Carmarthenshire
54
820
148
18.0%
Ceredigion
42
675
99
14.7%
Cornwall and Isles of
Scilly
1
85
3
3.5%
Devon
6
1,498
124
8.3%
North East Wales
45
1,262
123
9.7%
Pembrokeshire
134
2,061
297
14.4%
Powys
44
590
78
13.2%
South Wales
29
387
69
17.8%
Staffordshire
1
22
1
4.5%
Wiltshire
2
581
8
1.4%
TC0077
360
8,296
961
11.6 %
Table 8b Number of Cattle Antibody Tests 2020