Monthly report: Surveillence Project SB4008 IFNg tests for Bovine tuberculosis - February 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 162
Report Period 1st - 28th February 2020
Issued 16 March 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
February
2020
%
Total 2020
%
Submissions
323
666
Samples
Total (%)
33146
100.00 %
64420
100.00 %
England
27498
82.96 %
51745
80.32 %
Wales
5486
16.55 %
11243
17.45 %
Scotland
162
0.49 %
1432
2.22 %
Parallel Tests
PAR-CUL-N
12527
37.79 %
21288
33.05 %
PAR-CUL-P
1029
3.10 %
3073
4.77 %
PAR-HERD-S
204
0.62 %
226
0.35 %
PAR-NEW-IN
14045
42.37 %
27667
42.95 %
PAR-OTHER
241
0.73 %
837
1.30 %
PAR-PERSIS
4440
13.40 %
9508
14.76 %
PAR-RAPID
104
0.31 %
165
0.26 %
Total(% of all samples)
32590
98.32 %
62764
97.43 %
Serial/Extended Tests
SER-FLEXI
556
1.68 %
1656
2.57 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
Total(% of all samples)
556
1.68 %
1656
2.57 %
Total
33146
100.00 %
64420
100.00 %
Retests
Total (% of all samples)
1958
5.91 %
4026
6.25 %
England
1585
4.78 %
3131
4.86 %
Wales
368
1.11 %
816
1.27 %
Scotland
5
0.02 %
79
0.12 %
Resamples
Total (% of all samples)
1571
4.74 %
3182
4.94 %
England
1272
3.84 %
2446
3.80 %
Wales
294
0.89 %
669
1.04 %
Scotland
5
0.02 %
67
0.10 %
Rejects
Total (% of all samples)
987
2.98 %
1484
2.30 %
England
918
2.77 %
1405
2.18 %
Wales
4
0.01 %
14
0.02 %
Scotland
65
0.20 %
65
0.10 %
Table 1. Monthly Totals for February 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
12527
37.8 %
PAR-CUL-P
1029
3.1 %
PAR-HERD-S
204
0.6 %
PAR-NEW-IN
14045
42.4 %
PAR-OTHER
241
0.7 %
PAR-PERSIS
4440
13.4 %
PAR-RAPID
104
0.3 %
SER-FLEXI
556
1.7 %
Total
33146
100.00 %
Figure 1. Number of samples submitted in each category during February 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
Argyll & Bute
W Glamorgan
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Lincs
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Berks
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during February 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
12
2,133
6.44 %
49
2.30 %
2004
93.95 %
102
4.78 %
80
3.75 %
0
0.00 %
PAR-PERSIS
7
1,299
3.92 %
35
2.69 %
1210
93.15 %
69
5.31 %
53
4.08 %
1
0.08 %
Devon
PAR-CUL-N
34
3,394
10.24 %
122
3.59 %
3108
91.57 %
201
5.92 %
161
4.74 %
3
0.09 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Dorset
PAR-CUL-N
6
1,321
3.99 %
43
3.26 %
1080
81.76 %
54
4.09 %
47
3.56 %
151
11.43 %
PAR-CUL-P
4
1,029
3.10 %
131
12.73 %
866
84.16 %
53
5.15 %
31
3.01 %
1
0.10 %
PAR-NEW-IN
1
161
0.49 %
5
3.11 %
150
93.17 %
10
6.21 %
6
3.73 %
0
0.00 %
SER-FLEXI
3
349
1.05 %
19
5.44 %
323
92.55 %
13
3.72 %
7
2.01 %
0
0.00 %
Gloucs
PAR-CUL-N
10
1,183
3.57 %
38
3.21 %
1055
89.18 %
107
9.04 %
90
7.61 %
0
0.00 %
Heref
PAR-CUL-N
5
325
0.98 %
11
3.38 %
297
91.38 %
24
7.38 %
15
4.62 %
2
0.62 %
PAR-NEW-IN
1
4
0.01 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Shropshire
PAR-PERSIS
3
781
2.36 %
50
6.40 %
690
88.35 %
49
6.27 %
41
5.25 %
0
0.00 %
Somerset excl N
PAR-CUL-N
15
1,741
5.25 %
65
3.73 %
1625
93.34 %
71
4.08 %
51
2.93 %
0
0.00 %
Staffs
PAR-CUL-N
1
303
0.91 %
25
8.25 %
261
86.14 %
20
6.60 %
17
5.61 %
0
0.00 %
Wiltshire
PAR-CUL-N
8
2,127
6.42 %
75
3.53 %
1959
92.10 %
123
5.78 %
84
3.95 %
9
0.42 %
SER-FLEXI
1
19
0.06 %
1
5.26 %
18
94.74 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
2
23
0.07 %
2
8.70 %
21
91.30 %
2
8.70 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
19
4,299
12.97 %
79
1.84 %
3968
92.30 %
235
5.47 %
202
4.70 %
50
1.16 %
Derbyshire
PAR-NEW-IN
19
2,979
8.99 %
103
3.46 %
2329
78.18 %
240
8.06 %
216
7.25 %
331
11.11 %
E Sussex
PAR-NEW-IN
4
226
0.68 %
8
3.54 %
211
93.36 %
9
3.98 %
7
3.10 %
0
0.00 %
Hampshire
PAR-NEW-IN
4
363
1.10 %
16
4.41 %
327
90.08 %
20
5.51 %
20
5.51 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
30
0.09 %
0
0.00 %
14
46.67 %
1
3.33 %
1
3.33 %
15
50.00 %
Oxon
PAR-NEW-IN
15
1,537
4.64 %
58
3.77 %
1336
86.92 %
81
5.27 %
55
3.58 %
88
5.73 %
Warks
PAR-NEW-IN
10
1,246
3.76 %
27
2.17 %
1090
87.48 %
84
6.74 %
72
5.78 %
57
4.57 %
England - LRA
Cumbria
PAR-NEW-IN
1
30
0.09 %
0
0.00 %
29
96.67 %
2
6.67 %
1
3.33 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
211
0.64 %
0
0.00 %
1
0.47 %
0
0.00 %
0
0.00 %
210
99.53 %
Table 2a. Summary by County for February 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Lincs
PAR-NEW-IN
1
58
0.17 %
5
8.62 %
48
82.76 %
5
8.62 %
5
8.62 %
0
0.00 %
N Yorks
PAR-NEW-IN
1
72
0.22 %
1
1.39 %
71
98.61 %
0
0.00 %
0
0.00 %
0
0.00 %
Surrey
PAR-NEW-IN
1
253
0.76 %
5
1.98 %
239
94.47 %
9
3.56 %
9
3.56 %
0
0.00 %
England
193
27498
82.96 %
973
3.54 %
24335
88.50 %
1585
5.76 %
1272
4.63 %
918
3.34 %
Wales - High TB
Carms
PAR-HERD-S
2
194
0.59 %
17
8.76 %
171
88.14 %
13
6.70 %
6
3.09 %
0
0.00 %
PAR-OTHER
7
147
0.44 %
16
10.88 %
118
80.27 %
14
9.52 %
13
8.84 %
0
0.00 %
PAR-PERSIS
7
845
2.55 %
23
2.72 %
792
93.73 %
41
4.85 %
29
3.43 %
1
0.12 %
PAR-RAPID
6
25
0.08 %
6
24.00 %
16
64.00 %
3
12.00 %
3
12.00 %
0
0.00 %
SER-FLEXI
1
7
0.02 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-PERSIS
4
24
0.07 %
3
12.50 %
19
79.17 %
2
8.33 %
2
8.33 %
0
0.00 %
PAR-RAPID
2
8
0.02 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
5
149
0.45 %
16
10.74 %
129
86.58 %
10
6.71 %
4
2.68 %
0
0.00 %
PAR-RAPID
2
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
46
0.14 %
12
26.09 %
34
73.91 %
4
8.70 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
10
63
0.19 %
23
36.51 %
31
49.21 %
10
15.87 %
9
14.29 %
0
0.00 %
PAR-PERSIS
17
865
2.61 %
61
7.05 %
765
88.44 %
48
5.55 %
38
4.39 %
1
0.12 %
PAR-RAPID
7
31
0.09 %
5
16.13 %
26
83.87 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
2
6
0.02 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
400
1.21 %
20
5.00 %
348
87.00 %
35
8.75 %
30
7.50 %
2
0.50 %
PAR-OTHER
3
16
0.05 %
1
6.25 %
12
75.00 %
4
25.00 %
3
18.75 %
0
0.00 %
PAR-PERSIS
8
302
0.91 %
22
7.28 %
260
86.09 %
23
7.62 %
20
6.62 %
0
0.00 %
PAR-RAPID
7
29
0.09 %
4
13.79 %
25
86.21 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
53
0.16 %
3
5.66 %
48
90.57 %
3
5.66 %
2
3.77 %
0
0.00 %
W Glamorgan
PAR-PERSIS
1
12
0.04 %
0
0.00 %
10
83.33 %
2
16.67 %
2
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-PERSIS
1
150
0.45 %
22
14.67 %
120
80.00 %
8
5.33 %
8
5.33 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-PERSIS
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
46
0.14 %
5
10.87 %
39
84.78 %
2
4.35 %
2
4.35 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
970
2.93 %
22
2.27 %
897
92.47 %
61
6.29 %
51
5.26 %
0
0.00 %
PAR-PERSIS
3
12
0.04 %
1
8.33 %
9
75.00 %
2
16.67 %
2
16.67 %
0
0.00 %
PAR-RAPID
3
7
0.02 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
25
0.08 %
2
8.00 %
23
92.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
3
477
1.44 %
7
1.47 %
446
93.50 %
30
6.29 %
24
5.03 %
0
0.00 %
PAR-OTHER
1
12
0.04 %
0
0.00 %
10
83.33 %
3
25.00 %
2
16.67 %
0
0.00 %
S Glamorgan
SER-FLEXI
1
9
0.03 %
4
44.44 %
5
55.56 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
2
18
0.05 %
0
0.00 %
16
88.89 %
2
11.11 %
2
11.11 %
0
0.00 %
NE Wales
PAR-NEW-IN
2
526
1.59 %
9
1.71 %
476
90.49 %
47
8.94 %
41
7.79 %
0
0.00 %
Wales
129
5486
16.55 %
315
5.74 %
4873
88.83 %
368
6.71 %
294
5.36 %
4
0.07 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
162
0.49 %
1
0.62 %
91
56.17 %
5
3.09 %
5
3.09 %
65
40.12 %
Scotland
1
162
0.49 %
1
0.62 %
91
56.17 %
5
3.09 %
5
3.09 %
65
40.12 %
Grand Total
Sum:
323
33146
1289
29299
1958
1571
987
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
17
2,897
4.50 %
75
2.59 %
2702
93.27 %
147
5.07 %
120
4.14 %
0
0.00 %
PAR-CUL-P
2
351
0.54 %
22
6.27 %
314
89.46 %
17
4.84 %
15
4.27 %
0
0.00 %
PAR-PERSIS
15
3,240
5.03 %
133
4.10 %
3002
92.65 %
157
4.85 %
104
3.21 %
1
0.03 %
Devon
PAR-CUL-N
72
8,456
13.13 %
308
3.64 %
7605
89.94 %
534
6.32 %
439
5.19 %
104
1.23 %
PAR-CUL-P
6
723
1.12 %
34
4.70 %
630
87.14 %
72
9.96 %
59
8.16 %
0
0.00 %
SER-FLEXI
3
50
0.08 %
2
4.00 %
46
92.00 %
2
4.00 %
2
4.00 %
0
0.00 %
Dorset
PAR-CUL-N
14
1,958
3.04 %
137
7.00 %
1594
81.41 %
94
4.80 %
76
3.88 %
151
7.71 %
PAR-CUL-P
5
1,161
1.80 %
137
11.80 %
990
85.27 %
58
5.00 %
33
2.84 %
1
0.09 %
PAR-NEW-IN
1
161
0.25 %
5
3.11 %
150
93.17 %
10
6.21 %
6
3.73 %
0
0.00 %
SER-FLEXI
5
740
1.15 %
48
6.49 %
674
91.08 %
30
4.05 %
18
2.43 %
0
0.00 %
Gloucs
PAR-CUL-N
16
1,616
2.51 %
63
3.90 %
1430
88.49 %
149
9.22 %
123
7.61 %
0
0.00 %
Heref
PAR-CUL-N
9
877
1.36 %
33
3.76 %
812
92.59 %
43
4.90 %
29
3.31 %
3
0.34 %
PAR-NEW-IN
3
248
0.38 %
10
4.03 %
207
83.47 %
38
15.32 %
30
12.10 %
1
0.40 %
PAR-PERSIS
2
213
0.33 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-PERSIS
6
996
1.55 %
60
6.02 %
882
88.55 %
62
6.22 %
52
5.22 %
2
0.20 %
SER-FLEXI
3
389
0.60 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
29
2,323
3.61 %
88
3.79 %
2128
91.61 %
110
4.74 %
79
3.40 %
28
1.21 %
PAR-CUL-P
1
261
0.41 %
13
4.98 %
238
91.19 %
13
4.98 %
10
3.83 %
0
0.00 %
Staffs
PAR-CUL-N
1
303
0.47 %
25
8.25 %
261
86.14 %
20
6.60 %
17
5.61 %
0
0.00 %
Wiltshire
PAR-CUL-N
16
2,497
3.88 %
85
3.40 %
2278
91.23 %
165
6.61 %
120
4.81 %
14
0.56 %
PAR-CUL-P
3
577
0.90 %
35
6.07 %
523
90.64 %
32
5.55 %
19
3.29 %
0
0.00 %
PAR-PERSIS
1
97
0.15 %
5
5.15 %
88
90.72 %
5
5.15 %
4
4.12 %
0
0.00 %
SER-FLEXI
2
45
0.07 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
7
120
0.19 %
6
5.00 %
108
90.00 %
16
13.33 %
6
5.00 %
0
0.00 %
Bucks
PAR-NEW-IN
6
296
0.46 %
6
2.03 %
237
80.07 %
55
18.58 %
53
17.91 %
0
0.00 %
Cheshire
PAR-NEW-IN
42
7,939
12.32 %
177
2.23 %
7395
93.15 %
401
5.05 %
317
3.99 %
50
0.63 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Derbyshire
PAR-NEW-IN
36
4,836
7.51 %
171
3.54 %
3928
81.22 %
360
7.44 %
305
6.31 %
432
8.93 %
E Sussex
PAR-NEW-IN
9
678
1.05 %
30
4.42 %
630
92.92 %
24
3.54 %
18
2.65 %
0
0.00 %
Hampshire
PAR-CUL-N
1
361
0.56 %
15
4.16 %
327
90.58 %
39
10.80 %
19
5.26 %
0
0.00 %
PAR-NEW-IN
6
623
0.97 %
20
3.21 %
563
90.37 %
44
7.06 %
40
6.42 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
9
622
0.97 %
21
3.38 %
558
89.71 %
35
5.63 %
28
4.50 %
15
2.41 %
Northants
PAR-NEW-IN
5
298
0.46 %
8
2.68 %
272
91.28 %
22
7.38 %
18
6.04 %
0
0.00 %
Notts
PAR-NEW-IN
1
75
0.12 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Oxon
PAR-NEW-IN
29
2,984
4.63 %
121
4.05 %
2550
85.46 %
182
6.10 %
125
4.19 %
188
6.30 %
Warks
PAR-NEW-IN
16
1,903
2.95 %
44
2.31 %
1672
87.86 %
110
5.78 %
86
4.52 %
101
5.31 %
England - LRA
Cumbria
PAR-NEW-IN
1
30
0.05 %
0
0.00 %
29
96.67 %
2
6.67 %
1
3.33 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
3
237
0.37 %
1
0.42 %
25
10.55 %
1
0.42 %
1
0.42 %
210
88.61 %
Lincs
PAR-NEW-IN
1
58
0.09 %
5
8.62 %
48
82.76 %
5
8.62 %
5
8.62 %
0
0.00 %
Norfolk
PAR-NEW-IN
2
40
0.06 %
1
2.50 %
20
50.00 %
6
15.00 %
6
15.00 %
13
32.50 %
N Yorks
PAR-NEW-IN
2
213
0.33 %
2
0.94 %
120
56.34 %
1
0.47 %
0
0.00 %
91
42.72 %
Surrey
PAR-NEW-IN
1
253
0.39 %
5
1.98 %
239
94.47 %
9
3.56 %
9
3.56 %
0
0.00 %
England
409
51745
80.32 %
1982
3.83 %
45912
88.73 %
3131
6.05 %
2446
4.73 %
1405
2.72 %
Wales - High TB
Carms
PAR-HERD-S
3
201
0.31 %
17
8.46 %
178
88.56 %
13
6.47 %
6
2.99 %
0
0.00 %
PAR-OTHER
9
194
0.30 %
23
11.86 %
157
80.93 %
15
7.73 %
14
7.22 %
0
0.00 %
PAR-PERSIS
14
1,428
2.22 %
94
6.58 %
1288
90.20 %
64
4.48 %
45
3.15 %
1
0.07 %
PAR-RAPID
13
38
0.06 %
10
26.32 %
24
63.16 %
4
10.53 %
4
10.53 %
0
0.00 %
SER-FLEXI
1
7
0.01 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
8
0.01 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-PERSIS
10
416
0.65 %
16
3.85 %
381
91.59 %
21
5.05 %
17
4.09 %
2
0.48 %
PAR-RAPID
5
18
0.03 %
5
27.78 %
13
72.22 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
10
458
0.71 %
24
5.24 %
424
92.58 %
21
4.59 %
10
2.18 %
0
0.00 %
PAR-RAPID
5
10
0.02 %
2
20.00 %
8
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
133
0.21 %
17
12.78 %
113
84.96 %
7
5.26 %
3
2.26 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Pembs
PAR-HERD-S
2
5
0.01 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-OTHER
18
603
0.94 %
65
10.78 %
511
84.74 %
36
5.97 %
27
4.48 %
0
0.00 %
PAR-PERSIS
28
1,811
2.81 %
123
6.79 %
1567
86.53 %
136
7.51 %
117
6.46 %
4
0.22 %
PAR-RAPID
14
58
0.09 %
14
24.14 %
43
74.14 %
1
1.72 %
1
1.72 %
0
0.00 %
SER-FLEXI
2
35
0.05 %
0
0.00 %
33
94.29 %
4
11.43 %
2
5.71 %
0
0.00 %
Powys
PAR-HERD-S
5
16
0.02 %
2
12.50 %
14
87.50 %
2
12.50 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
1,273
1.98 %
50
3.93 %
1083
85.07 %
148
11.63 %
133
10.45 %
7
0.55 %
PAR-OTHER
3
16
0.02 %
1
6.25 %
12
75.00 %
4
25.00 %
3
18.75 %
0
0.00 %
PAR-PERSIS
16
416
0.65 %
37
8.89 %
314
75.48 %
71
17.07 %
65
15.63 %
0
0.00 %
PAR-RAPID
7
29
0.05 %
4
13.79 %
25
86.21 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
137
0.21 %
5
3.65 %
126
91.97 %
8
5.84 %
6
4.38 %
0
0.00 %
S Wales
PAR-PERSIS
1
69
0.11 %
3
4.35 %
64
92.75 %
6
8.70 %
2
2.90 %
0
0.00 %
SER-FLEXI
1
36
0.06 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-PERSIS
1
12
0.02 %
0
0.00 %
10
83.33 %
2
16.67 %
2
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
198
0.31 %
23
11.62 %
167
84.34 %
8
4.04 %
8
4.04 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
2
7
0.01 %
4
57.14 %
2
28.57 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
46
0.07 %
5
10.87 %
39
84.78 %
2
4.35 %
2
4.35 %
0
0.00 %
NE Wales
PAR-NEW-IN
16
1,864
2.89 %
42
2.25 %
1717
92.11 %
122
6.55 %
105
5.63 %
0
0.00 %
PAR-PERSIS
6
147
0.23 %
28
19.05 %
112
76.19 %
7
4.76 %
7
4.76 %
0
0.00 %
PAR-RAPID
4
8
0.01 %
3
37.50 %
5
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
25
0.04 %
2
8.00 %
23
92.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
5
569
0.88 %
8
1.41 %
535
94.02 %
35
6.15 %
26
4.57 %
0
0.00 %
PAR-OTHER
1
12
0.02 %
0
0.00 %
10
83.33 %
3
25.00 %
2
16.67 %
0
0.00 %
S Glamorgan
SER-FLEXI
3
13
0.02 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Low TB
Gwynedd
PAR-NEW-IN
3
53
0.08 %
2
3.77 %
45
84.91 %
6
11.32 %
6
11.32 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
3
862
1.34 %
10
1.16 %
803
93.16 %
63
7.31 %
49
5.68 %
0
0.00 %
Wales
246
11243
17.45 %
650
5.78 %
9910
88.14 %
816
7.26 %
669
5.95 %
14
0.12 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
162
0.25 %
1
0.62 %
91
56.17 %
5
3.09 %
5
3.09 %
65
40.12 %
Ayrshire
PAR-NEW-IN
3
383
0.59 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
3
569
0.88 %
8
1.41 %
538
94.55 %
26
4.57 %
23
4.04 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
1
21
0.03 %
0
0.00 %
21
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
297
0.46 %
14
4.71 %
267
89.90 %
18
6.06 %
16
5.39 %
0
0.00 %
Scotland
11
1432
2.22 %
24
1.68 %
1276
89.11 %
79
5.52 %
67
4.68 %
65
4.54 %
Grand Total
Sum:
666
64420
2656
57098
4026
3182
1484
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol February 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
188
27128
98.7%
953
3.5%
23992
88.4%
1572
5.8%
1265
4.7%
918
3.4%
Serial/Extended
5
370
1.3%
20
5.4%
343
92.7%
13
3.5%
7
1.9%
0
0.0 %
Total
193
27498
100.0%
973
3.5%
24335
88.5%
1585
5.8%
1272
4.6%
918
3.3%
Scotland
Parallel
1
162
100.0%
1
0.6%
91
56.2%
5
3.1%
5
3.1%
65
40.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
162
100.0%
1
0.6%
91
56.2%
5
3.1%
5
3.1%
65
40.1%
Wales
Parallel
120
5300
96.6%
286
5.4%
4720
89.1%
359
6.8%
290
5.5%
4
0.1%
Serial/Extended
9
186
3.4%
29
15.6%
153
82.3%
9
4.8%
4
2.2%
0
0.0 %
Total
129
5486
100.0%
315
5.7%
4873
88.8%
368
6.7%
294
5.4%
4
0.1%
GB
Parallel
309
32590
98.3%
1240
3.8%
28803
88.4%
1936
5.9%
1560
4.8%
987
3.0%
Serial/Extended
14
556
1.7%
49
8.8%
496
89.2%
22
4.0%
11
2.0%
0
0.0 %
Total
323
33146
100.0%
1289
3.9%
29299
88.4%
1958
5.9%
1571
4.7%
987
3.0%
193.00
129.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
396
50521
97.6%
1912
3.8%
44799
88.7%
3072
6.1%
2405
4.8%
1405
2.8%
Serial/Extended
13
1224
2.4%
70
5.7%
1113
90.9%
59
4.8%
41
3.3%
0
0.0 %
Total
409
51745
100.0%
1982
3.8%
45912
88.7%
3131
6.1%
2446
4.7%
1405
2.7%
Scotland
Parallel
11
1432
100.0%
24
1.7%
1276
89.1%
79
5.5%
67
4.7%
65
4.5%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
11
1432
100.0%
24
1.7%
1276
89.1%
79
5.5%
67
4.7%
65
4.5%
Wales
Parallel
227
10811
96.2%
613
5.7%
9528
88.1%
795
7.4%
656
6.1%
14
0.1%
Serial/Extended
19
432
3.8%
37
8.6%
382
88.4%
21
4.9%
13
3.0%
0
0.0 %
Total
246
11243
100.0%
650
5.8%
9910
88.1%
816
7.3%
669
6.0%
14
0.1%
GB
Parallel
634
62764
97.4%
2549
4.1%
55603
88.6%
3946
6.3%
3128
5.0%
1484
2.4%
Serial/Extended
32
1656
2.6%
107
6.5%
1495
90.3%
80
4.8%
54
3.3%
0
0.0 %
Total
666
64420
100.0%
2656
4.1%
57098
88.6%
4026
6.2%
3182
4.9%
1484
2.3%
409.00
246.00
11.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
14
556
1.7%
49
8.8%
496
89.2%
22
4.0 %
11
2.0 %
0
Parallel - Herd or Group Slaughter
5
204
0.6%
18
8.8%
180
88.2%
13
6.4 %
6
2.9 %
0
Parallel New Infection
101
14045
42.4%
368
2.6%
12111
86.2%
879
6.3 %
748
5.3 %
818
5.8 %
Parallel - new infection cull area
91
12527
37.8%
428
3.4%
11389
90.9%
702
5.6 %
545
4.4 %
165
1.3 %
Parallel - Other
23
241
0.7%
40
16.6%
173
71.8%
32
13.3 %
28
11.6 %
0
Parallel Persistent Infection
57
4440
13.4%
234
5.3%
4004
90.2%
254
5.7 %
199
4.5 %
3
0.1 %
Parallel - persistent infection cull area
4
1029
3.1%
131
12.7%
866
84.2%
53
5.2 %
31
3.0 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
28
104
0.3%
21
20.2%
80
76.9%
3
2.9 %
3
2.9 %
0
Total
323
33146
100.0%
1289
3.9%
29299
88.4%
1958
5.9 %
1571
4.7 %
987
3.0 %
Table 4a. Summaryof IFN Gamma results by submission reason (February 2020)
England - HRA
Extended Flexible IFN-GAMMA test
5
370
1.1%
20
5.4%
343
92.7%
13
3.5 %
7
1.9 %
0
Parallel New Infection
2
165
0.5%
5
3.0%
153
92.7%
11
6.7 %
7
4.2 %
0
Parallel - new infection cull area
91
12527
37.8%
428
3.4%
11389
90.9%
702
5.6 %
545
4.4 %
165
1.3 %
Parallel Persistent Infection
10
2080
6.3%
85
4.1%
1900
91.3%
118
5.7 %
94
4.5 %
1
0.0 %
Parallel - persistent infection cull area
4
1029
3.1%
131
12.7%
866
84.2%
53
5.2 %
31
3.0 %
1
0.1 %
England - HRA
112
16171
48.8%
669
4.1%
14651
90.6%
897
5.5 %
684
4.2 %
167
1.0 %
England - Edge
Parallel New Infection
75
10703
32.3%
293
2.7%
9296
86.9%
672
6.3 %
573
5.4 %
541
5.1 %
England - Edge
75
10703
32.3%
293
2.7%
9296
86.9%
672
6.3 %
573
5.4 %
541
5.1 %
England - LRA
Parallel New Infection
6
624
1.9%
11
1.8%
388
62.2%
16
2.6 %
15
2.4 %
210
33.7 %
England - LRA
6
624
1.9%
11
1.8%
388
62.2%
16
2.6 %
15
2.4 %
210
33.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
6
106
0.3%
18
17.0%
86
81.1%
7
6.6 %
2
1.9 %
0
Parallel - Herd or Group Slaughter
5
204
0.6%
18
8.8%
180
88.2%
13
6.4 %
6
2.9 %
0
Parallel New Infection
3
400
1.2%
20
5.0%
348
87.0%
35
8.8 %
30
7.5 %
2
0.5 %
Parallel - Other
22
229
0.7%
40
17.5%
163
71.2%
29
12.7 %
26
11.4 %
0
Parallel Persistent Infection
42
2197
6.6%
125
5.7%
1975
89.9%
126
5.7 %
95
4.3 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
24
96
0.3%
17
17.7%
76
79.2%
3
3.1 %
3
3.1 %
0
Wales - High TB
102
3232
9.8%
238
7.4%
2828
87.5%
213
6.6 %
162
5.0 %
4
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
80
0.2%
11
13.8%
67
83.8%
2
2.5 %
2
2.5 %
0
Parallel New Infection
10
1447
4.4%
29
2.0%
1343
92.8%
91
6.3 %
75
5.2 %
0
Parallel - Other
1
12
0.0%
0
0.0 %
10
83.3%
3
25.0 %
2
16.7 %
0
Parallel Persistent Infection
5
163
0.5%
24
14.7%
129
79.1%
10
6.1 %
10
6.1 %
0
Parallel - Rapid Testing Two-times IRs
4
8
0.0%
4
50.0%
4
50.0%
0
0
0
Wales - Intermediate TB
23
1710
5.2%
68
4.0%
1553
90.8%
106
6.2 %
89
5.2 %
0
Wales - Low TB
Parallel New Infection
4
544
1.6%
9
1.7%
492
90.4%
49
9.0 %
43
7.9 %
0
Wales - Low TB
4
544
1.6%
9
1.7%
492
90.4%
49
9.0 %
43
7.9 %
0
Scotland - Scotland
Parallel New Infection
1
162
0.5%
1
0.6%
91
56.2%
5
3.1 %
5
3.1 %
65
40.1 %
Scotland - Scotland
1
162
0.5%
1
0.6%
91
56.2%
5
3.1 %
5
3.1 %
65
40.1 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
32
1656
2.6%
107
6.5%
1495
90.3%
80
4.8 %
54
3.3 %
0
Parallel - Herd or Group Slaughter
11
226
0.4%
20
8.8%
199
88.1%
16
7.1 %
7
3.1 %
0
Parallel New Infection
228
27667
42.9%
771
2.8%
24282
87.8%
1776
6.4 %
1441
5.2 %
1173
4.2 %
Parallel - new infection cull area
175
21288
33.0%
829
3.9%
19137
89.9%
1301
6.1 %
1022
4.8 %
300
1.4 %
Parallel - Other
36
837
1.3%
89
10.6%
697
83.3%
63
7.5 %
51
6.1 %
0
Parallel Persistent Infection
115
9508
14.8%
559
5.9%
8473
89.1%
593
6.2 %
466
4.9 %
10
0.1 %
Parallel - persistent infection cull area
17
3073
4.8%
241
7.8%
2695
87.7%
192
6.2 %
136
4.4 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
52
165
0.3%
40
24.2%
120
72.7%
5
3.0 %
5
3.0 %
0
Total
666
64420
100.0%
2656
4.1%
57098
88.6%
4026
6.2 %
3182
4.9 %
1484
2.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
13
1224
1.9%
70
5.7%
1113
90.9%
59
4.8 %
41
3.3 %
0
Parallel New Infection
4
409
0.6%
15
3.7%
357
87.3%
48
11.7 %
36
8.8 %
1
0.2 %
Parallel - new infection cull area
174
20927
32.5%
814
3.9%
18810
89.9%
1262
6.0 %
1003
4.8 %
300
1.4 %
Parallel Persistent Infection
24
4546
7.1%
207
4.6%
4144
91.2%
256
5.6 %
192
4.2 %
3
0.1 %
Parallel - persistent infection cull area
17
3073
4.8%
241
7.8%
2695
87.7%
192
6.2 %
136
4.4 %
1
0.0 %
England - Edge
Parallel New Infection
166
20374
31.6%
606
3.0%
17985
88.3%
1251
6.1 %
997
4.9 %
786
3.9 %
Parallel - new infection cull area
1
361
0.6%
15
4.2%
327
90.6%
39
10.8 %
19
5.3 %
0
England - LRA
Parallel New Infection
10
831
1.3%
14
1.7%
481
57.9%
24
2.9 %
22
2.6 %
314
37.8 %
England
409
51745
80.3%
1982
3.8%
45912
88.7%
3131
6.1 %
2446
4.7 %
1405
2.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
14
348
0.5%
25
7.2%
312
89.7%
19
5.5 %
11
3.2 %
0
Parallel - Herd or Group Slaughter
11
226
0.4%
20
8.8%
199
88.1%
16
7.1 %
7
3.1 %
0
Parallel New Infection
10
1273
2.0%
50
3.9%
1083
85.1%
148
11.6 %
133
10.4 %
7
0.5 %
Parallel - Other
34
824
1.3%
89
10.8%
686
83.3%
60
7.3 %
49
5.9 %
0
Parallel Persistent Infection
80
4610
7.2%
297
6.4%
4048
87.8%
321
7.0 %
258
5.6 %
7
0.2 %
Parallel - Rapid Testing Two-times IRs
44
153
0.2%
35
22.9%
113
73.9%
5
3.3 %
5
3.3 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
84
0.1%
12
14.3%
70
83.3%
2
2.4 %
2
2.4 %
0
Parallel New Infection
21
2433
3.8%
50
2.1%
2252
92.6%
157
6.5 %
131
5.4 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2020) Cont.
Parallel - Other
2
13
0.0%
0
0.0 %
11
84.6%
3
23.1 %
2
15.4 %
0
Parallel Persistent Infection
11
352
0.5%
55
15.6%
281
79.8%
16
4.5 %
16
4.5 %
0
Parallel - Rapid Testing Two-times IRs
7
11
0.0%
5
45.5%
6
54.5%
0
0
0
Wales - Low TB
Parallel New Infection
6
915
1.4%
12
1.3%
848
92.7%
69
7.5 %
55
6.0 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
246
11243
17.5%
650
5.8%
9910
88.1%
816
7.3 %
669
6.0 %
14
0.1 %
Scotland - Scotland
Parallel New Infection
11
1432
2.2%
24
1.7%
1276
89.1%
79
5.5 %
67
4.7 %
65
4.5 %
Scotland
11
1432
2.2%
24
1.7%
1276
89.1%
79
5.5 %
67
4.7 %
65
4.5 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
1
0
4
0
773
0
73
0
0
July
316
32549
201
1219
1
13
0
0
0
0
108
3
0
12
0
1020
0
58
0
2
August
262
21887
126
724
1
8
0
0
0
0
84
1
0
6
0
570
0
52
0
0
September
245
25317
134
693
1
7
0
0
0
0
94
1
0
3
0
542
0
45
0
0
October
298
26690
154
743
0
0
0
0
0
58
1
0
5
0
604
0
71
0
3
November
272
30186
169
1080
14
0
0
0
0
72
2
0
15
3
910
1
61
0
0
December
234
25192
138
904
2
0
0
0
0
0
87
0
1
4
1
759
2
41
0
7
2020 January
343
31274
227
1367
1
8
0
0
0
0
178
0
11
0
0
1109
57
0
0
1
February
323
33146
192
1289
29
644
0
0
0
0
62
0
3
0
0
508
41
0
0
0
Totals (last
12 months)
3528
340829
2037
12780
41
718
0
0
0
0
1158
12
15
63
4
10062
107
570
0
16
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2113
272219
1319
9648
552
0
0
0
0
1019
7
7
42
1
7899
8
64
0
1
Scotland
33
4125
17
114
2
0
0
0
0
13
0
1
2
0
90
0
5
0
0
Wales
1382
64485
701
3018
164
0
0
0
0
126
5
7
19
3
2073
99
501
0
15
Totals
3528
340829
2037
12780
718
0
0
0
0
1158
12
15
63
4
10062
107
570
0
16
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
35
Wiltshire
Warks
Staffs
Shropshire
Powys
Oxon
NE Wales
Leics & Rut
Hampshire
Gwent
Gloucs
Dorset
Derbyshire
Corn & Scilly
Ceredigion
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during February 2020 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
3.09 %
3.09 %
Berks
8.70 %
Carms
4.31 %
5.77 %
Ceredigion
5.81 %
5.81 %
Cheshire
4.70 %
5.47 %
Corn & Scilly
3.88 %
4.98 %
Cumbria
3.33 %
6.67 %
Derbyshire
7.25 %
8.06 %
Devon
4.74 %
5.92 %
Dorset
3.18 %
4.55 %
E Sussex
3.10 %
3.98 %
Gloucs
7.61 %
9.04 %
Gr Manchester
Gwent
2.02 %
7.07 %
Gwynedd
11.11 %
11.11 %
Hampshire
5.51 %
5.51 %
Heref
4.86 %
7.60 %
Leics & Rut
3.33 %
3.33 %
Lincs
8.62 %
8.62 %
NE Wales
6.10 %
7.14 %
N Yorks
Oxon
3.58 %
5.27 %
Pembs
4.90 %
6.05 %
Powys
6.25 %
7.57 %
S Glamorgan
Shropshire
5.25 %
6.27 %
Somerset excl
2.93 %
4.08 %
Staffs
5.61 %
6.60 %
Surrey
3.56 %
3.56 %
Warks
5.78 %
6.74 %
W Glamorgan
16.67 %
16.67 %
Wiltshire
3.91 %
5.73 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
26941
973
3.6%
27498
973
3.5%
Wales
5430
315
5.8%
5486
315
5.7%
Scotland
162
1
0.6%
162
1
0.6%
GB
32533
1289
4.0%
33146
1289
3.9%
Table 6a (i): Number of animals and submissions tested in February 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
49708
1982
4.0%
51745
1982
3.8%
Wales
10872
650
6.0%
11243
650
5.8%
Scotland
1387
24
1.7%
1432
24
1.7%
GB
61967
2656
4.3%
64420
2656
4.1%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22388
1471
6.6%
22715
1471
6.5%
Wales
5155
436
8.5%
5329
436
8.2%
GB
27543
1907
6.9%
28044
1907
6.8%
Table 6a (ii): Number of animals and submissions tested in February 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
41359
2360
5.7%
43300
2360
5.5%
Wales
11725
830
7.1%
12257
830
6.8%
GB
53084
3190
6.0%
55557
3190
5.7%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cheshire
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
Dorset
PRV-SP
1
3
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Oxfordshire
PRV-SP
1
32
0
0
Total
5
163
3
1.84 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples February 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
1
3
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Oxfordshire
PRV-SP
1
32
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
9
195
3
1.54 %
Isle of Man
SER-IOM
8
46
0
0
Isle of Man
SER-IOM
2
32
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
5
166
24
14.5%
Ceredigion
1
2
0
0
Devon
4
402
53
13.2%
North East Wales
3
92
12
13.0%
Pembrokeshire
6
169
24
14.2%
South Wales
3
10
1
10.0%
TC0077
22
841
114
13.6 %
Table 8a Number of Cattle Antibody Tests February 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
6
183
26
14.2%
Ceredigion
1
2
0
0
Devon
4
1,032
95
9.2%
North East Wales
6
135
18
13.3%
Pembrokeshire
9
237
33
13.9%
South Wales
4
45
4
8.9%
TC0077
30
1,634
176
10.8 %
Table 8b Number of Cattle Antibody Tests 2020