Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - August 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 168
Report Period 1st - 31st August 2020
Issued 10 September 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
August 2020
%
Total 2020
%
Submissions
290
2205
Samples
Total (%)
24676
100.00 %
181655
100.00 %
England
20621
83.57 %
143595
79.05 %
Wales
3686
14.94 %
33558
18.47 %
Scotland
369
1.50 %
4502
2.48 %
Parallel Tests
PAR-CUL-N
11793
47.79 %
65730
36.18 %
PAR-CUL-P
923
3.74 %
6697
3.69 %
PAR-HERD-S
63
0.26 %
1111
0.61 %
PAR-NEW-IN
7648
30.99 %
78487
43.21 %
PAR-OTHER
902
3.66 %
4066
2.24 %
PAR-PERSIS
2434
9.86 %
21235
11.69 %
PAR-RAPID
210
0.85 %
1073
0.59 %
Total(% of all samples)
23973
97.15 %
178399
98.21 %
Serial/Extended Tests
SER-FLEXI
687
2.78 %
3237
1.78 %
SER-FRAUD
16
0.06 %
19
0.01 %
SER-NSR
0
0.00 %
Total(% of all samples)
703
2.85 %
3256
1.79 %
Total
24676
100.00 %
181655
100.00 %
Retests
Total (% of all samples)
1496
6.06 %
10923
6.01 %
England
1301
5.27 %
8579
4.72 %
Wales
155
0.63 %
2069
1.14 %
Scotland
40
0.16 %
275
0.15 %
Resamples
Total (% of all samples)
1109
4.49 %
8342
4.59 %
England
963
3.90 %
6508
3.58 %
Wales
119
0.48 %
1617
0.89 %
Scotland
27
0.11 %
217
0.12 %
Rejects
Total (% of all samples)
406
1.65 %
2312
1.27 %
England
403
1.63 %
2210
1.22 %
Wales
3
0.01 %
37
0.02 %
Scotland
0
0.00 %
65
0.04 %
Table 1. Monthly Totals for August 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-FRAUD
Breakdown of
Reason for
submission
PAR-CUL-N
11793
47.8 %
PAR-CUL-P
923
3.7 %
PAR-HERD-S
63
0.3 %
PAR-NEW-IN
7648
31.0 %
PAR-OTHER
902
3.7 %
PAR-PERSIS
2434
9.9 %
PAR-RAPID
210
0.9 %
SER-FLEXI
687
2.8 %
SER-FRAUD
16
0.1 %
Total
24676
100.00 %
Figure 1. Number of samples submitted in each category during August 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Argyll & Bute
W Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Notts
Northants
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Berks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during August 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
Wigtown
Perthshire
Kirkcudbright
Dumfries & G
Berwickshire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
SER-FLEXI
1
66
0.27 %
1
1.52 %
58
87.88 %
7
10.61 %
7
10.61 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
13
1,644
6.66 %
24
1.46 %
1562
95.01 %
88
5.35 %
58
3.53 %
0
0.00 %
SER-FLEXI
2
372
1.51 %
9
2.42 %
354
95.16 %
10
2.69 %
9
2.42 %
0
0.00 %
Devon
PAR-CUL-N
29
4,385
17.77 %
177
4.04 %
3959
90.29 %
280
6.39 %
229
5.22 %
20
0.46 %
PAR-CUL-P
2
179
0.73 %
25
13.97 %
146
81.56 %
11
6.15 %
6
3.35 %
2
1.12 %
Dorset
PAR-CUL-N
7
1,992
8.07 %
49
2.46 %
1851
92.92 %
119
5.97 %
92
4.62 %
0
0.00 %
PAR-CUL-P
2
375
1.52 %
21
5.60 %
346
92.27 %
12
3.20 %
8
2.13 %
0
0.00 %
PAR-PERSIS
1
470
1.90 %
33
7.02 %
423
90.00 %
17
3.62 %
14
2.98 %
0
0.00 %
SER-FLEXI
3
65
0.26 %
4
6.15 %
60
92.31 %
2
3.08 %
1
1.54 %
0
0.00 %
Gloucs
PAR-CUL-N
6
774
3.14 %
18
2.33 %
682
88.11 %
96
12.40 %
72
9.30 %
2
0.26 %
PAR-OTHER
1
56
0.23 %
1
1.79 %
48
85.71 %
8
14.29 %
7
12.50 %
0
0.00 %
Heref
PAR-NEW-IN
4
378
1.53 %
13
3.44 %
352
93.12 %
18
4.76 %
13
3.44 %
0
0.00 %
Shropshire
PAR-CUL-N
4
1,384
5.61 %
25
1.81 %
1315
95.01 %
69
4.99 %
44
3.18 %
0
0.00 %
Somerset excl N
PAR-CUL-N
8
715
2.90 %
26
3.64 %
635
88.81 %
86
12.03 %
54
7.55 %
0
0.00 %
Staffs
PAR-CUL-N
2
134
0.54 %
4
2.99 %
123
91.79 %
10
7.46 %
7
5.22 %
0
0.00 %
PAR-NEW-IN
8
908
3.68 %
113
12.44 %
760
83.70 %
40
4.41 %
34
3.74 %
1
0.11 %
Wiltshire
PAR-CUL-N
8
765
3.10 %
9
1.18 %
733
95.82 %
39
5.10 %
23
3.01 %
0
0.00 %
PAR-CUL-P
1
369
1.50 %
18
4.88 %
326
88.35 %
35
9.49 %
24
6.50 %
1
0.27 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
16
0.06 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
1
74
0.30 %
1
1.35 %
68
91.89 %
5
6.76 %
5
6.76 %
0
0.00 %
Cheshire
PAR-NEW-IN
11
2,384
9.66 %
35
1.47 %
2282
95.72 %
91
3.82 %
67
2.81 %
0
0.00 %
Derbyshire
PAR-NEW-IN
7
800
3.24 %
14
1.75 %
441
55.13 %
41
5.13 %
29
3.62 %
316
39.50 %
E Sussex
PAR-NEW-IN
3
265
1.07 %
6
2.26 %
246
92.83 %
11
4.15 %
13
4.91 %
0
0.00 %
Hampshire
PAR-NEW-IN
5
530
2.15 %
9
1.70 %
475
89.62 %
73
13.77 %
46
8.68 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
7
535
2.17 %
42
7.85 %
460
85.98 %
42
7.85 %
33
6.17 %
0
0.00 %
Table 2a. Summary by County for August 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for August 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
3
219
0.89 %
6
2.74 %
199
90.87 %
14
6.39 %
14
6.39 %
0
0.00 %
Notts
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Oxon
PAR-NEW-IN
7
162
0.66 %
4
2.47 %
138
85.19 %
29
17.90 %
20
12.35 %
0
0.00 %
Warks
PAR-NEW-IN
5
119
0.48 %
5
4.20 %
103
86.55 %
20
xx-xxxx-xxxx %
11
9.24 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
4
480
1.95 %
3
0.63 %
394
82.08 %
27
5.63 %
22
4.58 %
61
12.71 %
England
159
20621
83.57 %
695
3.37 %
18560
90.01 %
1301
6.31 %
963
4.67 %
403
1.95 %
Wales - High TB
Carms
PAR-OTHER
4
54
0.22 %
9
16.67 %
45
83.33 %
1
1.85 %
0
0.00 %
0
0.00 %
PAR-RAPID
15
53
0.21 %
9
xx-xxxx-xxxx %
41
77.36 %
3
5.66 %
3
5.66 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
9
0.04 %
0
0.00 %
3
33.33 %
6
66.67 %
6
66.67 %
0
0.00 %
PAR-OTHER
3
24
0.10 %
4
16.67 %
19
79.17 %
3
12.50 %
1
4.17 %
0
0.00 %
PAR-PERSIS
1
10
0.04 %
0
0.00 %
5
50.00 %
5
50.00 %
5
50.00 %
0
0.00 %
PAR-RAPID
3
7
0.03 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-RAPID
3
46
0.19 %
5
10.87 %
39
84.78 %
2
4.35 %
2
4.35 %
0
0.00 %
SER-FLEXI
1
115
0.47 %
5
4.35 %
107
93.04 %
3
2.61 %
3
2.61 %
0
0.00 %
Pembs
PAR-OTHER
13
128
0.52 %
20
15.63 %
102
79.69 %
7
5.47 %
6
4.69 %
0
0.00 %
PAR-PERSIS
15
1,024
4.15 %
31
3.03 %
970
94.73 %
31
3.03 %
23
2.25 %
0
0.00 %
PAR-RAPID
10
26
0.11 %
8
30.77 %
17
65.38 %
1
3.85 %
1
3.85 %
0
0.00 %
SER-FLEXI
2
69
0.28 %
1
1.45 %
65
94.20 %
4
5.80 %
3
4.35 %
0
0.00 %
Powys
PAR-HERD-S
1
11
0.04 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-PERSIS
2
24
0.10 %
2
8.33 %
22
91.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
9
34
0.14 %
9
26.47 %
24
70.59 %
1
2.94 %
1
2.94 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-PERSIS
3
64
0.26 %
8
12.50 %
52
81.25 %
5
7.81 %
4
6.25 %
0
0.00 %
PAR-RAPID
2
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
8
0.03 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
9
488
1.98 %
11
2.25 %
473
96.93 %
7
1.43 %
4
0.82 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for August 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
9
256
1.04 %
21
8.20 %
221
86.33 %
17
6.64 %
14
5.47 %
0
0.00 %
PAR-PERSIS
4
696
2.82 %
16
2.30 %
661
94.97 %
24
3.45 %
16
2.30 %
3
0.43 %
PAR-RAPID
4
6
0.02 %
4
66.67 %
2
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
1
4
0.02 %
0
0.00 %
2
50.00 %
3
75.00 %
2
50.00 %
0
0.00 %
PAR-OTHER
1
8
0.03 %
0
0.00 %
5
62.50 %
6
75.00 %
3
37.50 %
0
0.00 %
PAR-PERSIS
1
146
0.59 %
1
0.68 %
140
95.89 %
7
4.79 %
5
3.42 %
0
0.00 %
W Glamorgan
PAR-RAPID
1
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
43
0.17 %
0
0.00 %
42
97.67 %
1
2.33 %
1
2.33 %
0
0.00 %
PAR-NEW-IN
4
296
1.20 %
13
4.39 %
271
91.55 %
14
4.73 %
12
4.05 %
0
0.00 %
PAR-OTHER
1
6
0.02 %
0
0.00 %
3
50.00 %
3
50.00 %
3
50.00 %
0
0.00 %
NE Wales
PAR-RAPID
2
19
0.08 %
1
5.26 %
18
94.74 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
129
3686
14.94 %
189
5.13 %
3375
91.56 %
155
4.21 %
119
3.23 %
3
0.08 %
Scotland - Scotland
Argyll & Bute
PAR-OTHER
2
369
1.50 %
4
1.08 %
338
91.60 %
40
10.84 %
27
7.32 %
0
0.00 %
Scotland
2
369
1.50 %
4
1.08 %
338
91.60 %
40
10.84 %
27
7.32 %
0
0.00 %
Grand Total
Sum:
290
24676
888
22273
1496
1109
406
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
SER-FLEXI
1
66
0.04 %
1
1.52 %
58
87.88 %
7
10.61 %
7
10.61 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
84
10,306
5.67 %
315
3.06 %
9432
91.52 %
631
6.12 %
459
4.45 %
100
0.97 %
PAR-CUL-P
7
1,802
0.99 %
46
2.55 %
xx-xxxx-xxxx
94.28 %
73
4.05 %
57
3.16 %
0
0.00 %
PAR-PERSIS
17
3,332
1.83 %
135
4.05 %
3079
92.41 %
171
5.13 %
117
3.51 %
1
0.03 %
SER-FLEXI
2
372
0.20 %
9
2.42 %
354
95.16 %
10
2.69 %
9
2.42 %
0
0.00 %
Devon
PAR-CUL-N
202
22,893
12.60 %
1190
5.20 %
20467
89.40 %
1420
6.20 %
1106
4.83 %
130
0.57 %
PAR-CUL-P
10
1,069
0.59 %
59
5.52 %
939
87.84 %
89
8.33 %
69
6.45 %
2
0.19 %
PAR-PERSIS
1
92
0.05 %
8
8.70 %
80
86.96 %
8
8.70 %
4
4.35 %
0
0.00 %
SER-FLEXI
6
312
0.17 %
7
2.24 %
288
92.31 %
14
4.49 %
11
3.53 %
6
1.92 %
Dorset
PAR-CUL-N
42
6,448
3.55 %
362
5.61 %
5684
88.15 %
333
5.16 %
251
3.89 %
151
2.34 %
PAR-CUL-P
9
2,347
1.29 %
224
9.54 %
2020
86.07 %
146
6.22 %
102
4.35 %
1
0.04 %
PAR-NEW-IN
2
167
0.09 %
5
2.99 %
156
93.41 %
11
6.59 %
6
3.59 %
0
0.00 %
PAR-PERSIS
1
470
0.26 %
33
7.02 %
423
90.00 %
17
3.62 %
14
2.98 %
0
0.00 %
SER-FLEXI
8
805
0.44 %
52
6.46 %
734
91.18 %
32
3.98 %
19
2.36 %
0
0.00 %
Gloucs
PAR-CUL-N
37
3,399
1.87 %
150
4.41 %
2999
88.23 %
307
9.03 %
248
7.30 %
2
0.06 %
PAR-NEW-IN
1
38
0.02 %
4
10.53 %
32
84.21 %
4
10.53 %
2
5.26 %
0
0.00 %
PAR-OTHER
1
56
0.03 %
1
1.79 %
48
85.71 %
8
14.29 %
7
12.50 %
0
0.00 %
PAR-PERSIS
2
242
0.13 %
14
5.79 %
220
90.91 %
10
4.13 %
8
3.31 %
0
0.00 %
Heref
PAR-CUL-N
17
2,508
1.38 %
90
3.59 %
2331
92.94 %
117
4.67 %
82
3.27 %
5
0.20 %
PAR-NEW-IN
7
626
0.34 %
23
3.67 %
559
89.30 %
56
8.95 %
43
6.87 %
1
0.16 %
PAR-PERSIS
2
213
0.12 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-CUL-N
10
2,369
1.30 %
45
1.90 %
2243
94.68 %
115
4.85 %
79
3.33 %
2
0.08 %
PAR-PERSIS
7
1,015
0.56 %
60
5.91 %
899
88.57 %
65
6.40 %
54
5.32 %
2
0.20 %
SER-FLEXI
3
389
0.21 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
83
8,345
4.59 %
360
4.31 %
7698
92.25 %
404
4.84 %
258
3.09 %
29
0.35 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
1
261
0.14 %
13
4.98 %
238
91.19 %
13
4.98 %
10
3.83 %
0
0.00 %
PAR-PERSIS
3
293
0.16 %
25
8.53 %
258
88.05 %
14
4.78 %
10
3.41 %
0
0.00 %
Staffs
PAR-CUL-N
8
1,102
0.61 %
41
3.72 %
852
77.31 %
61
5.54 %
54
4.90 %
155
14.07 %
PAR-NEW-IN
46
6,233
3.43 %
305
4.89 %
5707
91.56 %
283
4.54 %
220
3.53 %
1
0.02 %
Wiltshire
PAR-CUL-N
54
7,129
3.92 %
186
2.61 %
6566
92.10 %
475
6.66 %
357
5.01 %
20
0.28 %
PAR-CUL-P
6
1,218
0.67 %
69
5.67 %
1083
88.92 %
83
6.81 %
55
4.52 %
11
0.90 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
104
0.06 %
5
4.81 %
93
89.42 %
7
6.73 %
6
5.77 %
0
0.00 %
SER-FLEXI
2
45
0.02 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
19
1,901
1.05 %
54
2.84 %
1720
90.48 %
163
8.57 %
127
6.68 %
0
0.00 %
Bucks
PAR-NEW-IN
12
1,457
0.80 %
62
4.26 %
1297
89.02 %
120
8.24 %
98
6.73 %
0
0.00 %
Cheshire
PAR-CUL-N
6
868
0.48 %
14
1.61 %
837
96.43 %
28
3.23 %
17
1.96 %
0
0.00 %
PAR-NEW-IN
110
17,692
9.74 %
380
2.15 %
16546
93.52 %
911
5.15 %
716
4.05 %
50
0.28 %
Derbyshire
PAR-NEW-IN
103
11,427
6.29 %
356
3.12 %
9686
84.76 %
785
6.87 %
633
5.54 %
752
6.58 %
E Sussex
PAR-NEW-IN
22
1,668
0.92 %
58
3.48 %
1537
92.15 %
84
5.04 %
73
4.38 %
0
0.00 %
Hampshire
PAR-CUL-N
2
363
0.20 %
15
4.13 %
327
90.08 %
39
10.74 %
19
5.23 %
2
0.55 %
PAR-NEW-IN
24
2,495
1.37 %
51
2.04 %
2298
92.10 %
211
8.46 %
146
5.85 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
39
3,810
2.10 %
157
4.12 %
3464
90.92 %
242
6.35 %
171
4.49 %
18
0.47 %
Northants
PAR-NEW-IN
17
1,014
0.56 %
48
4.73 %
915
90.24 %
60
5.92 %
50
4.93 %
1
0.10 %
Notts
PAR-NEW-IN
6
366
0.20 %
4
1.09 %
350
95.63 %
17
4.64 %
11
3.01 %
1
0.27 %
Oxon
PAR-NEW-IN
79
7,378
4.06 %
337
4.57 %
6410
86.88 %
470
6.37 %
343
4.65 %
288
3.90 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Warks
PAR-NEW-IN
44
3,714
2.04 %
97
2.61 %
3351
90.23 %
216
5.82 %
162
4.36 %
104
2.80 %
PAR-PERSIS
2
479
0.26 %
20
4.18 %
446
93.11 %
20
4.18 %
13
2.71 %
0
0.00 %
England - LRA
Cleveland
PAR-NEW-IN
1
50
0.03 %
2
4.00 %
48
96.00 %
1
2.00 %
0
0.00 %
0
0.00 %
Cumbria
PAR-NEW-IN
1
30
0.02 %
0
0.00 %
29
96.67 %
2
6.67 %
1
3.33 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
7
336
0.18 %
6
1.79 %
114
33.93 %
6
1.79 %
6
1.79 %
210
62.50 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Humberside
PAR-NEW-IN
2
78
0.04 %
7
8.97 %
66
84.62 %
7
8.97 %
5
6.41 %
0
0.00 %
Lincs
PAR-NEW-IN
5
250
0.14 %
14
5.60 %
225
90.00 %
12
4.80 %
11
4.40 %
0
0.00 %
PAR-PERSIS
2
34
0.02 %
0
0.00 %
31
91.18 %
5
14.71 %
3
8.82 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
49
0.03 %
1
2.04 %
29
59.18 %
6
12.24 %
6
12.24 %
13
26.53 %
Northumb
PAR-NEW-IN
2
307
0.17 %
17
5.54 %
240
78.18 %
40
13.03 %
50
16.29 %
0
0.00 %
N Yorks
PAR-NEW-IN
8
794
0.44 %
15
1.89 %
659
83.00 %
40
5.04 %
29
3.65 %
91
11.46 %
Surrey
PAR-NEW-IN
3
429
0.24 %
17
3.96 %
396
92.31 %
18
4.20 %
16
3.73 %
0
0.00 %
W Yorks
PAR-NEW-IN
6
519
0.29 %
3
0.58 %
430
82.85 %
33
6.36 %
25
4.82 %
61
11.75 %
England
1214
143595
79.05 %
5601
3.90 %
129276
90.03 %
8579
5.97 %
6508
4.53 %
2210
1.54 %
Wales - High TB
Carms
PAR-HERD-S
12
678
0.37 %
51
7.52 %
598
88.20 %
45
6.64 %
29
4.28 %
0
0.00 %
PAR-OTHER
34
465
0.26 %
79
xx-xxxx-xxxx %
362
77.85 %
29
6.24 %
24
5.16 %
0
0.00 %
PAR-PERSIS
45
2,840
1.56 %
206
7.25 %
2509
88.35 %
156
5.49 %
120
4.23 %
5
0.18 %
PAR-RAPID
59
230
0.13 %
37
16.09 %
178
77.39 %
23
10.00 %
15
6.52 %
0
0.00 %
SER-FLEXI
6
84
0.05 %
22
26.19 %
59
70.24 %
2
2.38 %
1
1.19 %
2
2.38 %
Ceredigion
PAR-HERD-S
2
13
0.01 %
0
0.00 %
7
53.85 %
6
46.15 %
6
46.15 %
0
0.00 %
PAR-OTHER
27
324
0.18 %
22
6.79 %
286
88.27 %
23
7.10 %
16
4.94 %
0
0.00 %
PAR-PERSIS
21
866
0.48 %
44
5.08 %
780
90.07 %
47
5.43 %
40
4.62 %
2
0.23 %
PAR-RAPID
27
94
0.05 %
27
28.72 %
62
65.96 %
7
7.45 %
5
5.32 %
0
0.00 %
SER-FLEXI
2
40
0.02 %
0
0.00 %
38
95.00 %
2
5.00 %
2
5.00 %
0
0.00 %
Gwent
PAR-HERD-S
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
16
1,082
0.60 %
61
5.64 %
993
91.77 %
46
4.25 %
27
2.50 %
1
0.09 %
PAR-RAPID
16
108
0.06 %
18
16.67 %
87
80.56 %
3
2.78 %
3
2.78 %
0
0.00 %
SER-FLEXI
4
248
0.14 %
22
8.87 %
220
88.71 %
10
4.03 %
6
2.42 %
0
0.00 %
Pembs
PAR-HERD-S
7
292
0.16 %
11
3.77 %
271
92.81 %
12
4.11 %
10
3.42 %
0
0.00 %
PAR-OTHER
82
1,211
0.67 %
154
12.72 %
1011
83.48 %
62
5.12 %
46
3.80 %
0
0.00 %
PAR-PERSIS
101
6,080
3.35 %
287
4.72 %
5508
90.59 %
347
5.71 %
277
4.56 %
8
0.13 %
PAR-RAPID
86
382
0.21 %
85
22.25 %
285
74.61 %
16
4.19 %
12
3.14 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
17
377
0.21 %
15
3.98 %
349
92.57 %
20
5.31 %
13
3.45 %
0
0.00 %
Powys
PAR-HERD-S
10
52
0.03 %
6
11.54 %
46
88.46 %
2
3.85 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
113
0.06 %
6
5.31 %
106
93.81 %
1
0.88 %
1
0.88 %
0
0.00 %
PAR-OTHER
12
776
0.43 %
42
5.41 %
659
84.92 %
83
10.70 %
75
9.66 %
0
0.00 %
PAR-PERSIS
47
1,480
0.81 %
94
6.35 %
1233
83.31 %
165
11.15 %
144
9.73 %
9
0.61 %
PAR-RAPID
36
119
0.07 %
19
15.97 %
97
81.51 %
4
3.36 %
3
2.52 %
0
0.00 %
SER-FLEXI
10
212
0.12 %
17
8.02 %
186
87.74 %
19
8.96 %
9
4.25 %
0
0.00 %
S Wales
PAR-PERSIS
2
274
0.15 %
12
4.38 %
260
94.89 %
8
2.92 %
2
0.73 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
36
0.02 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-PERSIS
3
16
0.01 %
0
0.00 %
14
87.50 %
3
18.75 %
2
12.50 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
30
0.02 %
5
16.67 %
24
80.00 %
3
10.00 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
1
155
0.09 %
34
21.94 %
119
76.77 %
2
1.29 %
2
1.29 %
0
0.00 %
PAR-OTHER
7
29
0.02 %
5
17.24 %
23
79.31 %
1
3.45 %
1
3.45 %
0
0.00 %
PAR-PERSIS
6
262
0.14 %
31
11.83 %
219
83.59 %
13
4.96 %
12
4.58 %
0
0.00 %
PAR-RAPID
6
9
0.00 %
5
55.56 %
4
44.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
171
0.09 %
12
7.02 %
152
88.89 %
8
4.68 %
7
4.09 %
0
0.00 %
PAR-RAPID
4
18
0.01 %
6
33.33 %
12
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
48
0.03 %
6
12.50 %
40
83.33 %
2
4.17 %
2
4.17 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
74
7,121
3.92 %
203
2.85 %
6547
91.94 %
448
6.29 %
370
5.20 %
1
0.01 %
PAR-OTHER
20
327
0.18 %
26
7.95 %
284
86.85 %
21
6.42 %
17
5.20 %
0
0.00 %
PAR-PERSIS
16
1,547
0.85 %
90
5.82 %
1406
90.89 %
62
4.01 %
48
3.10 %
3
0.19 %
PAR-RAPID
19
60
0.03 %
15
25.00 %
42
70.00 %
3
5.00 %
3
5.00 %
0
0.00 %
SER-FLEXI
8
181
0.10 %
7
3.87 %
165
91.16 %
13
7.18 %
7
3.87 %
2
1.10 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-NEW-IN
12
913
0.50 %
18
1.97 %
848
92.88 %
57
6.24 %
46
5.04 %
1
0.11 %
PAR-OTHER
4
103
0.06 %
1
0.97 %
89
86.41 %
18
17.48 %
13
12.62 %
0
0.00 %
PAR-PERSIS
3
280
0.15 %
4
1.43 %
260
92.86 %
18
6.43 %
16
5.71 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
13
0.01 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
1
7
0.00 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
43
0.02 %
0
0.00 %
42
97.67 %
1
2.33 %
1
2.33 %
0
0.00 %
PAR-NEW-IN
26
1,667
0.92 %
30
1.80 %
1567
94.00 %
94
5.64 %
67
4.02 %
3
0.18 %
PAR-OTHER
2
7
0.00 %
0
0.00 %
3
42.86 %
4
57.14 %
4
57.14 %
0
0.00 %
PAR-RAPID
2
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
15
1,964
1.08 %
28
1.43 %
1840
93.69 %
142
7.23 %
96
4.89 %
0
0.00 %
PAR-OTHER
2
9
0.00 %
1
11.11 %
6
66.67 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-PERSIS
1
40
0.02 %
0
0.00 %
32
80.00 %
10
25.00 %
8
20.00 %
0
0.00 %
PAR-RAPID
3
20
0.01 %
1
5.00 %
19
95.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
944
33558
18.47 %
1879
5.60 %
30025
89.47 %
2069
6.17 %
1617
4.82 %
37
0.11 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
7
588
0.32 %
32
5.44 %
455
77.38 %
41
6.97 %
36
6.12 %
65
11.05 %
PAR-OTHER
6
755
0.42 %
15
1.99 %
682
90.33 %
83
10.99 %
58
7.68 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
383
0.21 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Berwickshire
PAR-NEW-IN
2
78
0.04 %
8
10.26 %
62
79.49 %
8
10.26 %
8
10.26 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
6
849
0.47 %
12
1.41 %
802
94.46 %
38
4.48 %
35
4.12 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
14
806
0.44 %
22
2.73 %
762
94.54 %
33
4.09 %
22
2.73 %
0
0.00 %
PAR-PERSIS
1
22
0.01 %
0
0.00 %
22
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Perthshire
PAR-NEW-IN
2
357
0.20 %
0
0.00 %
345
96.64 %
14
3.92 %
12
3.36 %
0
0.00 %
Wigtown
PAR-NEW-IN
6
664
0.37 %
17
2.56 %
624
93.98 %
28
4.22 %
23
3.46 %
0
0.00 %
Scotland
47
4502
2.48 %
107
2.38 %
4113
91.36 %
275
6.11 %
217
4.82 %
65
1.44 %
Grand Total
Sum:
xx-xxxx-xxxx
7587
163414
10923
8342
2312
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol August 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
152
20102
97.5%
681
3.4%
18072
89.9%
1282
6.4%
946
4.7%
403
2.0%
Serial/Extended
7
519
2.5%
14
2.7%
488
94.0%
19
3.7%
17
3.3%
0
0.0 %
Total
159
20621
100.0%
695
3.4%
18560
90.0%
1301
6.3%
963
4.7%
403
2.0%
Scotland
Parallel
2
369
100.0%
4
1.1%
338
91.6%
40
10.8%
27
7.3%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
369
100.0%
4
1.1%
338
91.6%
40
10.8%
27
7.3%
0
0.0 %
Wales
Parallel
126
3502
95.0%
183
5.2%
3203
91.5%
148
4.2%
113
3.2%
3
0.1%
Serial/Extended
3
184
5.0%
6
3.3%
172
93.5%
7
3.8%
6
3.3%
0
0.0 %
Total
129
3686
100.0%
189
5.1%
3375
91.6%
155
4.2%
119
3.2%
3
0.1%
GB
Parallel
280
23973
97.2%
868
3.6%
21613
90.2%
1470
6.1%
1086
4.5%
406
1.7%
Serial/Extended
10
703
2.8%
20
2.8%
660
93.9%
26
3.7%
23
3.3%
0
0.0 %
Total
290
24676
100.0%
888
3.6%
22273
90.3%
1496
6.1%
1109
4.5%
406
1.6%
159.00
129.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
1190 141587
98.6%
5512
3.9% 127430
90.0%
8489
6.0%
6441
4.5%
2204
1.6%
Serial/Extended
24
2008
1.4%
89
4.4%
1846
91.9%
90
4.5%
67
3.3%
6
0.3%
Total
xx-xxxx-xxxx
100.0%
5601
3.9% 129276
90.0%
8579
6.0%
6508
4.5%
2210
1.5%
Scotland
Parallel
47
4502
100.0%
107
2.4%
4113
91.4%
275
6.1%
217
4.8%
65
1.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
47
4502
100.0%
107
2.4%
4113
91.4%
275
6.1%
217
4.8%
65
1.4%
Wales
Parallel
890
32310
96.3%
1784
5.5%
28916
89.5%
2001
6.2%
1577
4.9%
33
0.1%
Serial/Extended
54
1248
3.7%
95
7.6%
1109
88.9%
68
5.4%
40
3.2%
4
0.3%
Total
944
33558
100.0%
1879
5.6%
30025
89.5%
2069
6.2%
1617
4.8%
37
0.1%
GB
Parallel
xx-xxxx-xxxx
98.2%
7403
4.1% 160459
89.9%
10765
6.0%
8235
4.6%
2302
1.3%
Serial/Extended
78
3256
1.8%
184
5.7%
2955
90.8%
158
4.9%
107
3.3%
10
0.3%
Total
xx-xxxx-xxxx
100.0%
7587
4.2% 163414
90.0%
10923
6.0%
8342
4.6%
2312
1.3%
#######
944.00
47.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
687
2.8%
20
2.9%
644
93.7%
26
3.8 %
23
3.3 %
0
Parallel - Herd or Group Slaughter
3
63
0.3%
2
3.2%
54
85.7%
7
11.1 %
7
11.1 %
0
Parallel New Infection
82
7648
31.0%
275
3.6%
6669
87.2%
436
5.7 %
326
4.3 %
378
4.9 %
Parallel - new infection cull area
77
11793
47.8%
332
2.8%
10860
92.1%
787
6.7 %
579
4.9 %
22
0.2 %
Parallel - Other
35
902
3.7%
59
6.5%
781
86.6%
86
9.5 %
62
6.9 %
0
Parallel Persistent Infection
27
2434
9.9%
91
3.7%
2273
93.4%
89
3.7 %
67
2.8 %
3
0.1 %
Parallel - persistent infection cull area
5
923
3.7%
64
6.9%
818
88.6%
58
6.3 %
38
4.1 %
3
0.3 %
Parallel - Rapid Testing Two-times IRs
51
210
0.9%
45
21.4%
158
75.2%
7
3.3 %
7
3.3 %
0
Serial - Anomalous Reaction
1
16
0.1%
0
0.0 %
16
100.0%
0
0
0
Total
290
24676
100.0%
888
3.6%
22273
90.3%
1496
6.1 %
1109
4.5 %
406
1.6 %
Table 4a. Summaryof IFN Gamma results by submission reason (August 2020)
England - HRA
Extended Flexible IFN-GAMMA test
6
503
2.0%
14
2.8%
472
93.8%
19
3.8 %
17
3.4 %
0
Parallel New Infection
13
1287
5.2%
126
9.8%
1113
86.5%
58
4.5 %
47
3.7 %
1
0.1 %
Parallel - new infection cull area
77
11793
47.8%
332
2.8%
10860
92.1%
787
6.7 %
579
4.9 %
22
0.2 %
Parallel - Other
1
56
0.2%
1
1.8%
48
85.7%
8
14.3 %
7
12.5 %
0
Parallel Persistent Infection
1
470
1.9%
33
7.0%
423
90.0%
17
3.6 %
14
3.0 %
0
Parallel - persistent infection cull area
5
923
3.7%
64
6.9%
818
88.6%
58
6.3 %
38
4.1 %
3
0.3 %
Serial - Anomalous Reaction
1
16
0.1%
0
0.0 %
16
100.0%
0
0
0
England - HRA
104
15048
61.0%
570
3.8%
13750
91.4%
947
6.3 %
702
4.7 %
26
0.2 %
England - Edge
Parallel New Infection
50
5090
20.6%
122
2.4%
4413
86.7%
327
6.4 %
239
4.7 %
316
6.2 %
England - Edge
50
5090
20.6%
122
2.4%
4413
86.7%
327
6.4 %
239
4.7 %
316
6.2 %
England - LRA
Parallel New Infection
5
483
2.0%
3
0.6%
397
82.2%
27
5.6 %
22
4.6 %
61
12.6 %
England - LRA
5
483
2.0%
3
0.6%
397
82.2%
27
5.6 %
22
4.6 %
61
12.6 %
Wales - High TB
Extended Flexible IFN-GAMMA test
3
184
0.7%
6
3.3%
172
93.5%
7
3.8 %
6
3.3 %
0
Parallel - Herd or Group Slaughter
2
20
0.1%
2
10.0%
12
60.0%
6
30.0 %
6
30.0 %
0
Parallel - Other
21
207
0.8%
33
15.9%
166
80.2%
12
5.8 %
8
3.9 %
0
Parallel Persistent Infection
18
1058
4.3%
33
3.1%
997
94.2%
36
3.4 %
28
2.6 %
0
Parallel - Rapid Testing Two-times IRs
40
166
0.7%
33
19.9%
126
75.9%
7
4.2 %
7
4.2 %
0
Wales - High TB
84
1635
6.6%
107
6.5%
1473
90.1%
68
4.2 %
55
3.4 %
0
Wales - Intermediate TB
Parallel New Infection
10
492
2.0%
11
2.2%
475
96.5%
10
2.0 %
6
1.2 %
0
Parallel - Other
10
264
1.1%
21
8.0%
226
85.6%
23
8.7 %
17
6.4 %
0
Parallel Persistent Infection
8
906
3.7%
25
2.8%
853
94.2%
36
4.0 %
25
2.8 %
3
0.3 %
Parallel - Rapid Testing Two-times IRs
9
25
0.1%
11
44.0%
14
56.0%
0
0
0
Wales - Intermediate TB
37
xx-xxxx-xxxx
6.8%
68
4.0%
1568
92.9%
69
4.1 %
48
2.8 %
3
0.2 %
Wales - Low TB
Parallel - Herd or Group Slaughter
1
43
0.2%
0
0.0 %
42
97.7%
1
2.3 %
1
2.3 %
0
Parallel New Infection
4
296
1.2%
13
4.4%
271
91.6%
14
4.7 %
12
4.1 %
0
Parallel - Other
1
6
0.0%
0
0.0 %
3
50.0%
3
50.0 %
3
50.0 %
0
Parallel - Rapid Testing Two-times IRs
2
19
0.1%
1
5.3%
18
94.7%
0
0
0
Wales - Low TB
8
364
1.5%
14
3.8%
334
91.8%
18
4.9 %
16
4.4 %
0
Scotland - Scotland
Parallel - Other
2
369
1.5%
4
1.1%
338
91.6%
40
10.8 %
27
7.3 %
0
Scotland - Scotland
2
369
1.5%
4
1.1%
338
91.6%
40
10.8 %
27
7.3 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
76
3237
1.8%
184
5.7%
2936
90.7%
158
4.9 %
107
3.3 %
10
0.3 %
Parallel - Herd or Group Slaughter
36
1111
0.6%
74
6.7%
990
89.1%
69
6.2 %
47
4.2 %
0
Parallel New Infection
740
78487
43.2%
2434
3.1%
70701
90.1%
4734
6.0 %
3691
4.7 %
1661
2.1 %
Parallel - new infection cull area
545
65730
36.2%
2768
4.2%
59436
90.4%
3930
6.0 %
2930
4.5 %
596
0.9 %
Parallel - Other
199
4066
2.2%
347
8.5%
3453
84.9%
337
8.3 %
266
6.5 %
0
Parallel Persistent Infection
306
21235
11.7%
1150
5.4%
19090
89.9%
1232
5.8 %
964
4.5 %
31
0.1 %
Parallel - persistent infection cull area
33
6697
3.7%
411
6.1%
5979
89.3%
404
6.0 %
293
4.4 %
14
0.2 %
Parallel - Rapid Testing Two-times IRs
268
1073
0.6%
219
20.4%
810
75.5%
59
5.5 %
44
4.1 %
0
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
7587
4.2%
163414
90.0%
10923
6.0 %
8342
4.6 %
2312
1.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
22
1989
1.1%
89
4.5%
1827
91.9%
90
4.5 %
67
3.4 %
6
0.3 %
Parallel New Infection
57
7065
3.9%
337
4.8%
6455
91.4%
354
5.0 %
271
3.8 %
2
0.0 %
Parallel - new infection cull area
537
64499
35.5%
2739
4.2%
58272
90.3%
3863
6.0 %
2894
4.5 %
594
0.9 %
Parallel - Other
1
56
0.0%
1
1.8%
48
85.7%
8
14.3 %
7
12.5 %
0
Parallel Persistent Infection
35
5761
3.2%
289
5.0%
5224
90.7%
324
5.6 %
245
4.3 %
3
0.1 %
Parallel - persistent infection cull area
33
6697
3.7%
411
6.1%
5979
89.3%
404
6.0 %
293
4.4 %
14
0.2 %
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
England - Edge
Parallel New Infection
475
52922
29.1%
1604
3.0%
47574
89.9%
3279
6.2 %
2530
4.8 %
1214
2.3 %
Parallel - new infection cull area
8
1231
0.7%
29
2.4%
1164
94.6%
67
5.4 %
36
2.9 %
2
0.2 %
Parallel Persistent Infection
3
480
0.3%
20
4.2%
447
93.1%
20
4.2 %
13
2.7 %
0
England - LRA
Parallel New Infection
39
2842
1.6%
82
2.9%
2236
78.7%
165
5.8 %
149
5.2 %
375
13.2 %
Parallel Persistent Infection
2
34
0.0%
0
0.0 %
31
91.2%
5
14.7 %
3
8.8 %
0
England
xx-xxxx-xxxx
79.0%
5601
3.9%
129276
90.0%
8579
6.0 %
6508
4.5 %
2210
1.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
41
1006
0.6%
77
7.7%
896
89.1%
53
5.3 %
31
3.1 %
2
0.2 %
Parallel - Herd or Group Slaughter
32
1038
0.6%
69
6.6%
924
89.0%
65
6.3 %
45
4.3 %
0
Parallel New Infection
1
113
0.1%
6
5.3%
106
93.8%
1
0.9 %
1
0.9 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2020) Cont.
Parallel - Other
157
2780
1.5%
298
10.7%
2318
83.4%
200
7.2 %
164
5.9 %
0
Parallel Persistent Infection
235
12638
7.0%
704
5.6%
11297
89.4%
772
6.1 %
612
4.8 %
25
0.2 %
Parallel - Rapid Testing Two-times IRs
228
941
0.5%
189
20.1%
714
75.9%
53
5.6 %
38
4.0 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
13
242
0.1%
18
7.4%
213
88.0%
15
6.2 %
9
3.7 %
2
0.8 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
87
8189
4.5%
255
3.1%
7514
91.8%
507
6.2 %
418
5.1 %
2
0.0 %
Parallel - Other
31
459
0.3%
32
7.0%
396
86.3%
40
8.7 %
31
6.8 %
0
Parallel Persistent Infection
29
2260
1.2%
137
6.1%
2037
90.1%
101
4.5 %
83
3.7 %
3
0.1 %
Parallel - Rapid Testing Two-times IRs
35
100
0.1%
29
29.0%
68
68.0%
3
3.0 %
3
3.0 %
0
Wales - Low TB
Parallel - Herd or Group Slaughter
1
43
0.0%
0
0.0 %
42
97.7%
1
2.3 %
1
2.3 %
0
Parallel New Infection
41
3631
2.0%
58
1.6%
3407
93.8%
236
6.5 %
163
4.5 %
3
0.1 %
Parallel - Other
4
16
0.0%
1
6.3%
9
56.3%
6
37.5 %
6
37.5 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
5
32
0.0%
1
3.1%
28
87.5%
3
9.4 %
3
9.4 %
0
Wales
944
33558
18.5%
1879
5.6%
30025
89.5%
2069
6.2 %
1617
4.8 %
37
0.1 %
Scotland - Scotland
Parallel New Infection
40
3725
2.1%
92
2.5%
3409
91.5%
192
5.2 %
159
4.3 %
65
1.7 %
Parallel - Other
6
755
0.4%
15
2.0%
682
90.3%
83
11.0 %
58
7.7 %
0
Parallel Persistent Infection
1
22
0.0%
0
0.0 %
22
100.0%
0
0
0
Scotland
47
4502
2.5%
107
2.4%
4113
91.4%
275
6.1 %
217
4.8 %
65
1.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 September
245
25317
134
693
1
7
0
0
0
0
94
1
0
3
0
542
0
45
0
0
October
298
26690
154
743
0
0
0
0
0
58
1
0
5
0
604
0
71
0
3
November
272
30186
169
1080
14
0
0
0
0
72
5
0
15
0
910
0
61
0
1
December
234
25192
138
904
2
0
0
0
0
0
87
2
0
4
0
759
0
49
0
1
2020 January
343
31274
227
1367
1
7
0
0
0
0
178
3
0
8
0
1110
1
57
0
0
February
323
33146
192
1289
1
21
0
0
0
0
187
3
0
6
0
982
0
89
0
0
March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
0
0
4
2
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
3
1
742
3
51
0
2
July
332
22622
180
1153
43
0
0
0
0
203
0
5
3
0
828
71
0
0
0
August
290
24676
173
888
139
294
0
0
0
0
94
0
0
0
0
252
39
0
0
0
Totals (last
12 months)
3254
289040
1886
11007
148
412
0
0
0
0
1245
19
5
58
3
8362
114
557
0
8
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1837
227376
1151
8041
316
0
0
0
0
1132
9
3
27
1
6320
32
23
0
0
Scotland
54
4985
25
114
5
0
0
0
0
12
0
0
5
0
75
0
17
0
0
Wales
1363
56679
710
2852
91
0
0
0
0
101
10
2
26
2
1967
82
517
0
8
Totals
3254
289040
1886
11007
412
0
0
0
0
1245
19
5
58
3
8362
114
557
0
8
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
W Yorks
W Glamorgan
Staffs
Shropshire
Pembs
Notts
NE Wales
Leics & Rut
Hampshire
Gwent
Gloucs
Dorset
Derbyshire
Cheshire
Carms
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during August 2020 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
7.32 %
10.84 %
Avon
10.61 %
10.61 %
Berks
6.76 %
6.76 %
Carms
4.02 %
5.17 %
Ceredigion
20.69 %
24.14 %
Cheshire
2.81 %
3.82 %
Corn & Scilly
3.32 %
4.86 %
Derbyshire
3.62 %
5.13 %
Devon
5.15 %
6.38 %
Dorset
3.96 %
5.17 %
E Sussex
4.91 %
4.15 %
Gloucs
9.52 %
12.53 %
Gr Manchester
Gwent
3.11 %
3.11 %
Gwynedd
4.64 %
5.22 %
Hampshire
8.68 %
13.77 %
Heref
3.44 %
4.76 %
Leics & Rut
6.17 %
7.85 %
Mid Glamorgan
NE Wales
2.32 %
3.28 %
Northants
6.39 %
6.39 %
Notts
50.00 %
50.00 %
Oxon
12.35 %
17.90 %
Pembs
2.65 %
3.45 %
Powys
5.26 %
7.89 %
Shropshire
3.18 %
4.99 %
Somerset excl
7.55 %
12.03 %
Staffs
3.93 %
4.80 %
Warks
9.24 %
xx-xxxx-xxxx %
W Glamorgan
Wiltshire
4.08 %
6.43 %
W Yorks
4.58 %
5.63 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20354
695
3.4%
20621
695
3.4%
Wales
3626
189
5.2%
3686
189
5.1%
Scotland
369
4
1.1%
369
4
1.1%
GB
24349
888
3.6%
24676
888
3.6%
Table 6a (i): Number of animals and submissions tested in August 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
137054
5601
4.1%
143595
5601
3.9%
Wales
31112
1878
6.0%
33558
1879
5.6%
Scotland
3635
107
2.9%
4502
107
2.4%
GB
171800
7586
4.4%
181655
7587
4.2%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of August
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
17871
511
2.9%
18297
511
2.8%
Wales
3203
211
6.6%
3236
211
6.5%
Scotland
354
2
0.6%
354
2
0.6%
GB
21428
724
3.4%
21887
724
3.3%
Table 6a (ii): Number of animals and submissions tested in August 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of August
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
165650
7586
4.6%
179993
7586
4.2%
Wales
38635
2225
5.8%
42378
2225
5.3%
Scotland
1889
83
4.4%
2210
83
3.8%
GB
206170
9894
4.8%
224581
9894
4.4%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cumbria
PRV-SP
1
1
0
0
Essex
PRV-SP
1
2
0
0
Hampshire
PRV-SP
1
3
0
0
Total
4
9
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples August 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SP
1
1
0
0
Cumbria
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
1
3
0
0
Essex
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Hampshire
PRV-SP
1
3
0
0
Oxfordshire
PRV-SP
1
32
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
13
204
3
1.47 %
Isle of Man
SER-IOM
12
135
0
0
Isle of Man
SER-IOM
1
4
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
8
77
14
18.2%
Ceredigion
2
30
7
23.3%
North East Wales
10
157
18
11.5%
Pembrokeshire
16
296
27
9.1%
Powys
5
54
5
9.3%
South Wales
2
133
21
15.8%
Staffordshire
1
22
1
4.5%
TC0077
44
769
93
12.1 %
Table 8a Number of Cattle Antibody Tests August 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
38
600
109
18.2%
Ceredigion
23
222
31
14.0%
Devon
4
1,032
95
9.2%
North East Wales
32
729
82
11.2%
Pembrokeshire
86
1,352
198
14.6%
Powys
24
341
59
17.3%
South Wales
17
328
60
18.3%
Staffordshire
1
22
1
4.5%
Wiltshire
1
291
3
1.0%
TC0077
226
4,917
638
13.0 %
Table 8b Number of Cattle Antibody Tests 2020