Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - March 2023
Source: environment.data.gov.uk (2024-07-03)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2023. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 199
Report Period 1st - 31st March 2023
Issued 10 April 2023

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
March 2023
%
Total 2023
%
Submissions
266
812
Samples
Total (%)
19894
100.00 %
61128
100.00 %
England
14014
70.44 %
40930
66.96 %
Wales
4695
23.60 %
17425
28.51 %
Scotland
1185
5.96 %
2773
4.54 %
Parallel Tests
PAR-CHR
0
0.00 %
PAR-CUL-N
1019
5.12 %
1830
2.99 %
PAR-CUL-P
539
2.71 %
539
0.88 %
PAR-HERD-S
396
1.99 %
396
0.65 %
PAR-IR-EX
37
0.19 %
139
0.23 %
PAR-IR-SEV
163
0.82 %
599
0.98 %
PAR-NEW-IN
7977
40.10 %
23714
38.79 %
PAR-OTHER
465
2.34 %
1337
2.19 %
PAR-PERSIS
1147
5.77 %
3518
5.76 %
PAR-POSTM
60
0.30 %
94
0.15 %
PAR-RAPID
78
0.39 %
234
0.38 %
PAR-RECUR
7716
38.79 %
26741
43.75 %
Total(% of all samples)
19597
98.51 %
59141
96.75 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
297
1.49 %
1987
3.25 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
297
1.49 %
1987
3.25 %
Total
19894
100.00 %
61128
100.00 %
Retests
Total (% of all samples)
1476
7.42 %
4959
8.11 %
England
1033
5.19 %
3151
5.15 %
Wales
318
1.60 %
1531
2.50 %
Scotland
125
0.63 %
277
0.45 %
Resamples
Total (% of all samples)
1268
6.37 %
4114
6.73 %
England
885
4.45 %
2708
4.43 %
Wales
271
1.36 %
1149
1.88 %
Scotland
112
0.56 %
257
0.42 %
Rejects
Total (% of all samples)
44
0.22 %
1497
2.45 %
England
7
0.04 %
1101
1.80 %
Wales
26
0.13 %
322
0.53 %
Scotland
11
0.06 %
74
0.12 %
Table 1. Monthly Totals for March 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
1019
5.1 %
PAR-CUL-P
539
2.7 %
PAR-HERD-S
396
2.0 %
PAR-IR-EX
37
0.2 %
PAR-IR-SEV
163
0.8 %
PAR-NEW-IN
7977
40.1 %
PAR-OTHER
465
2.3 %
PAR-PERSIS
1147
5.8 %
PAR-POSTM
60
0.3 %
PAR-RAPID
78
0.4 %
PAR-RECUR
7716
38.8 %
SER-FLEXI
297
1.5 %
Total
19894
100.00 %
Figure 1. Number of samples submitted in each category during March 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
3500
Wigtown
West lothian
Ayrshire
W Glamorgan
Powys
Pembs
NW Wales
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Cheshire
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Lincs
Leics & Rut
Heref
Hampshire
Gloucs
E Sussex
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during March 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
1000
2000
3000
4000
5000
6000
7000
8000
Wigtown
West lothian
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NW Wales
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Cheshire
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
1
117
0.59 %
0
0.00 %
112
95.73 %
13
11.11 %
5
4.27 %
0
0.00 %
PAR-CUL-P
1
539
2.71 %
31
5.75 %
481
89.24 %
32
5.94 %
26
4.82 %
1
0.19 %
PAR-OTHER
3
324
1.63 %
24
7.41 %
271
83.64 %
33
10.19 %
29
8.95 %
0
0.00 %
PAR-PERSIS
1
81
0.41 %
3
3.70 %
54
66.67 %
24
29.63 %
24
29.63 %
0
0.00 %
PAR-RECUR
8
1,287
6.47 %
39
3.03 %
1152
89.51 %
108
8.39 %
96
7.46 %
0
0.00 %
Devon
PAR-CUL-N
4
711
3.57 %
46
6.47 %
620
87.20 %
53
7.45 %
45
6.33 %
0
0.00 %
PAR-HERD-S
2
396
1.99 %
35
8.84 %
326
82.32 %
48
12.12 %
35
8.84 %
0
0.00 %
PAR-RECUR
12
2,261
11.37 %
156
6.90 %
2001
88.50 %
133
5.88 %
103
4.56 %
1
0.04 %
Gloucs
PAR-RECUR
1
7
0.04 %
0
0.00 %
4
57.14 %
3
42.86 %
3
42.86 %
0
0.00 %
Heref
PAR-OTHER
1
66
0.33 %
2
3.03 %
62
93.94 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-RECUR
2
405
2.04 %
18
4.44 %
366
90.37 %
25
6.17 %
21
5.19 %
0
0.00 %
Shropshire
PAR-RECUR
5
323
1.62 %
16
4.95 %
263
81.42 %
51
15.79 %
44
13.62 %
0
0.00 %
Somerset excl N
PAR-OTHER
1
11
0.06 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-PERSIS
2
296
1.49 %
47
15.88 %
226
76.35 %
25
8.45 %
23
7.77 %
0
0.00 %
PAR-RECUR
4
428
2.15 %
18
4.21 %
385
89.95 %
27
6.31 %
23
5.37 %
2
0.47 %
Staffs
PAR-OTHER
1
38
0.19 %
1
2.63 %
37
97.37 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
7
1,588
7.98 %
29
1.83 %
1430
90.05 %
142
8.94 %
128
8.06 %
1
0.06 %
Wiltshire
PAR-RECUR
2
88
0.44 %
1
1.14 %
80
90.91 %
10
11.36 %
7
7.95 %
0
0.00 %
England - Edge
Bucks
PAR-CUL-N
1
191
0.96 %
3
1.57 %
182
95.29 %
6
3.14 %
6
3.14 %
0
0.00 %
PAR-NEW-IN
3
684
3.44 %
9
1.32 %
656
95.91 %
19
2.78 %
18
2.63 %
1
0.15 %
Cheshire
PAR-OTHER
1
5
0.03 %
0
0.00 %
1
20.00 %
4
80.00 %
4
80.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
2
55
0.28 %
3
5.45 %
48
87.27 %
6
10.91 %
4
7.27 %
0
0.00 %
E Sussex
PAR-NEW-IN
5
366
1.84 %
6
1.64 %
340
92.90 %
24
6.56 %
20
5.46 %
0
0.00 %
Hampshire
PAR-NEW-IN
4
545
2.74 %
22
4.04 %
494
90.64 %
32
5.87 %
28
5.14 %
1
0.18 %
Leics & Rut
PAR-NEW-IN
7
1,234
6.20 %
36
2.92 %
1157
93.76 %
45
3.65 %
41
3.32 %
0
0.00 %
Notts
PAR-NEW-IN
2
9
0.05 %
1
11.11 %
5
55.56 %
3
33.33 %
3
33.33 %
0
0.00 %
Table 2a. Summary by County for March 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Oxon
PAR-PERSIS
1
235
1.18 %
14
5.96 %
205
87.23 %
16
6.81 %
16
6.81 %
0
0.00 %
PAR-RECUR
3
314
1.58 %
17
5.41 %
268
85.35 %
32
10.19 %
29
9.24 %
0
0.00 %
Warks
PAR-NEW-IN
6
662
3.33 %
22
3.32 %
619
93.50 %
30
4.53 %
21
3.17 %
0
0.00 %
PAR-RECUR
2
12
0.06 %
0
0.00 %
11
91.67 %
1
8.33 %
1
8.33 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
3
199
1.00 %
4
2.01 %
175
87.94 %
21
10.55 %
20
10.05 %
0
0.00 %
Lincs
PAR-NEW-IN
4
495
2.49 %
10
2.02 %
428
86.46 %
61
12.32 %
57
11.52 %
0
0.00 %
N Yorks
PAR-NEW-IN
1
42
0.21 %
1
2.38 %
39
92.86 %
3
7.14 %
2
4.76 %
0
0.00 %
England
103
14014
70.44 %
615
4.39 %
12507
89.25 %
1033
7.37 %
885
6.32 %
7
0.05 %
Wales - High TB
Carms
PAR-IR-EX
1
6
0.03 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
18
0.09 %
4
22.22 %
13
72.22 %
1
5.56 %
1
5.56 %
0
0.00 %
PAR-PERSIS
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RAPID
5
14
0.07 %
4
28.57 %
8
57.14 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-RECUR
1
116
0.58 %
1
0.86 %
109
93.97 %
6
5.17 %
5
4.31 %
1
0.86 %
SER-FLEXI
1
133
0.67 %
6
4.51 %
119
89.47 %
10
7.52 %
7
5.26 %
1
0.75 %
Ceredigion
PAR-IR-EX
2
11
0.06 %
1
9.09 %
8
72.73 %
2
18.18 %
2
18.18 %
0
0.00 %
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
3
0.02 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-RECUR
3
147
0.74 %
6
4.08 %
137
93.20 %
3
2.04 %
3
2.04 %
1
0.68 %
SER-FLEXI
2
14
0.07 %
2
14.29 %
7
50.00 %
6
42.86 %
5
35.71 %
0
0.00 %
Gwent
PAR-IR-SEV
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
110
0.55 %
4
3.64 %
104
94.55 %
4
3.64 %
2
1.82 %
0
0.00 %
Pembs
PAR-IR-EX
4
13
0.07 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
12
109
0.55 %
34
31.19 %
72
66.06 %
3
2.75 %
3
2.75 %
0
0.00 %
PAR-PERSIS
5
417
2.10 %
37
8.87 %
344
82.49 %
42
10.07 %
36
8.63 %
0
0.00 %
PAR-RAPID
9
30
0.15 %
5
16.67 %
23
76.67 %
4
13.33 %
2
6.67 %
0
0.00 %
PAR-RECUR
6
503
2.53 %
17
3.38 %
459
91.25 %
30
5.96 %
26
5.17 %
1
0.20 %
SER-FLEXI
3
38
0.19 %
2
5.26 %
33
86.84 %
3
7.89 %
3
7.89 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-IR-EX
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
6
0.03 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
231
1.16 %
6
2.60 %
188
81.39 %
37
16.02 %
35
15.15 %
2
0.87 %
W Glamorgan
PAR-OTHER
1
12
0.06 %
1
8.33 %
8
66.67 %
3
25.00 %
3
25.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
23
0.12 %
4
17.39 %
17
73.91 %
2
8.70 %
2
8.70 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
1
306
1.54 %
1
0.33 %
293
95.75 %
15
4.90 %
11
3.59 %
1
0.33 %
Mid Glamorgan
PAR-RAPID
1
2
0.01 %
1
50.00 %
0
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
28
0.14 %
4
14.29 %
24
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
11
519
2.61 %
4
0.77 %
480
92.49 %
35
6.74 %
33
6.36 %
2
0.39 %
PAR-OTHER
1
9
0.05 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
93
0.47 %
6
6.45 %
78
83.87 %
9
9.68 %
9
9.68 %
0
0.00 %
PAR-POSTM
4
40
0.20 %
3
7.50 %
37
92.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
16
0.08 %
2
12.50 %
13
81.25 %
1
6.25 %
1
6.25 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Powys
PAR-IR-EX
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
6
0.03 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
19
1,488
7.48 %
22
1.48 %
1391
93.48 %
77
5.17 %
61
4.10 %
14
0.94 %
NE Wales
PAR-IR-SEV
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
187
0.94 %
3
1.60 %
168
89.84 %
15
8.02 %
13
6.95 %
3
1.60 %
PAR-POSTM
7
20
0.10 %
2
10.00 %
18
90.00 %
1
5.00 %
0
0.00 %
0
0.00 %
NW Wales
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
149
4695
23.60 %
193
4.11 %
4205
89.56 %
318
6.77 %
271
5.77 %
26
0.55 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
298
1.50 %
33
11.07 %
214
71.81 %
58
19.46 %
51
17.11 %
0
0.00 %
West lothian
PAR-NEW-IN
4
359
1.80 %
6
1.67 %
320
89.14 %
24
6.69 %
22
6.13 %
11
3.06 %
Wigtown
PAR-NEW-IN
7
528
2.65 %
20
3.79 %
469
88.83 %
43
8.14 %
39
7.39 %
0
0.00 %
Scotland
14
1185
5.96 %
59
4.98 %
1003
84.64 %
125
10.55 %
112
9.45 %
11
0.93 %
Grand Total
Sum:
266
19894
867
17715
1476
1268
44
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
7
298
0.49 %
12
4.03 %
268
89.93 %
19
6.38 %
17
5.70 %
1
0.34 %
Corn & Scilly
PAR-CUL-N
3
926
1.51 %
60
6.48 %
804
86.83 %
80
8.64 %
62
6.70 %
0
0.00 %
PAR-CUL-P
1
539
0.88 %
31
5.75 %
481
89.24 %
32
5.94 %
26
4.82 %
1
0.19 %
PAR-OTHER
3
324
0.53 %
24
7.41 %
271
83.64 %
33
10.19 %
29
8.95 %
0
0.00 %
PAR-PERSIS
1
81
0.13 %
3
3.70 %
54
66.67 %
24
29.63 %
24
29.63 %
0
0.00 %
PAR-RECUR
20
5,983
9.79 %
247
4.13 %
5284
88.32 %
496
8.29 %
451
7.54 %
1
0.02 %
Devon
PAR-CUL-N
5
713
1.17 %
46
6.45 %
621
87.10 %
54
7.57 %
46
6.45 %
0
0.00 %
PAR-HERD-S
2
396
0.65 %
35
8.84 %
326
82.32 %
48
12.12 %
35
8.84 %
0
0.00 %
PAR-RECUR
24
4,812
7.87 %
339
7.04 %
3960
82.29 %
312
6.48 %
246
5.11 %
267
5.55 %
Dorset
PAR-RECUR
2
717
1.17 %
12
1.67 %
321
44.77 %
22
3.07 %
22
3.07 %
362
50.49 %
Gloucs
PAR-RECUR
6
541
0.89 %
25
4.62 %
479
88.54 %
43
7.95 %
37
6.84 %
0
0.00 %
Heref
PAR-OTHER
1
66
0.11 %
2
3.03 %
62
93.94 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-RECUR
14
3,052
4.99 %
178
5.83 %
2659
87.12 %
247
8.09 %
215
7.04 %
0
0.00 %
Shropshire
PAR-RECUR
26
1,927
3.15 %
100
5.19 %
1657
85.99 %
212
11.00 %
168
8.72 %
2
0.10 %
Somerset excl N
PAR-OTHER
1
11
0.02 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-PERSIS
2
296
0.48 %
47
15.88 %
226
76.35 %
25
8.45 %
23
7.77 %
0
0.00 %
PAR-RECUR
9
793
1.30 %
30
3.78 %
723
91.17 %
43
5.42 %
37
4.67 %
3
0.38 %
Staffs
PAR-OTHER
1
38
0.06 %
1
2.63 %
37
97.37 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
18
2,965
4.85 %
62
2.09 %
2585
87.18 %
313
10.56 %
288
9.71 %
30
1.01 %
Wiltshire
PAR-RECUR
7
904
1.48 %
41
4.54 %
832
92.04 %
46
5.09 %
29
3.21 %
2
0.22 %
England - Edge
Berks
PAR-NEW-IN
4
784
1.28 %
10
1.28 %
726
92.60 %
50
6.38 %
48
6.12 %
0
0.00 %
PAR-RECUR
1
132
0.22 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-CUL-N
1
191
0.31 %
3
1.57 %
182
95.29 %
6
3.14 %
6
3.14 %
0
0.00 %
PAR-NEW-IN
11
1,387
2.27 %
35
2.52 %
1298
93.58 %
56
4.04 %
53
3.82 %
1
0.07 %
Cheshire
PAR-OTHER
3
516
0.84 %
16
3.10 %
489
94.77 %
11
2.13 %
11
2.13 %
0
0.00 %
PAR-PERSIS
8
1,187
1.94 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
Table 2b. Summary by County for 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
215
0.35 %
2
0.93 %
201
93.49 %
15
6.98 %
12
5.58 %
0
0.00 %
Derbyshire
PAR-NEW-IN
5
431
0.71 %
17
3.94 %
376
87.24 %
44
10.21 %
38
8.82 %
0
0.00 %
PAR-RECUR
5
1,010
1.65 %
32
3.17 %
905
89.60 %
79
7.82 %
73
7.23 %
0
0.00 %
E Sussex
PAR-NEW-IN
8
605
0.99 %
15
2.48 %
544
89.92 %
56
9.26 %
46
7.60 %
0
0.00 %
Hampshire
PAR-NEW-IN
5
763
1.25 %
31
4.06 %
691
90.56 %
49
6.42 %
39
5.11 %
2
0.26 %
Leics & Rut
PAR-NEW-IN
12
1,884
3.08 %
56
2.97 %
1754
93.10 %
90
4.78 %
74
3.93 %
0
0.00 %
Northants
PAR-NEW-IN
6
786
1.29 %
17
2.16 %
598
76.08 %
63
8.02 %
53
6.74 %
118
15.01 %
Notts
PAR-NEW-IN
7
301
0.49 %
10
3.32 %
270
89.70 %
37
12.29 %
19
6.31 %
2
0.66 %
Oxon
PAR-NEW-IN
1
177
0.29 %
4
2.26 %
160
90.40 %
17
9.60 %
13
7.34 %
0
0.00 %
PAR-PERSIS
1
235
0.38 %
14
5.96 %
205
87.23 %
16
6.81 %
16
6.81 %
0
0.00 %
PAR-RECUR
5
373
0.61 %
28
7.51 %
314
84.18 %
36
9.65 %
31
8.31 %
0
0.00 %
Warks
PAR-NEW-IN
6
662
1.08 %
22
3.32 %
619
93.50 %
30
4.53 %
21
3.17 %
0
0.00 %
PAR-OTHER
1
19
0.03 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
19
100.00 %
PAR-RECUR
10
152
0.25 %
5
3.29 %
116
76.32 %
19
12.50 %
17
11.18 %
14
9.21 %
England - LRA
Cumbria
PAR-NEW-IN
8
1,716
2.81 %
55
3.21 %
1515
88.29 %
167
9.73 %
146
8.51 %
0
0.00 %
Essex
PAR-NEW-IN
4
353
0.58 %
1
0.28 %
173
49.01 %
4
1.13 %
2
0.57 %
177
50.14 %
Gr Manchester
PAR-PERSIS
1
41
0.07 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
75
0.12 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Lincs
PAR-NEW-IN
7
895
1.46 %
17
1.90 %
809
90.39 %
73
8.16 %
69
7.71 %
0
0.00 %
N Yorks
PAR-NEW-IN
2
82
0.13 %
1
1.22 %
78
95.12 %
4
4.88 %
3
3.66 %
0
0.00 %
Surrey
PAR-NEW-IN
4
507
0.83 %
22
4.34 %
358
70.61 %
29
5.72 %
29
5.72 %
98
19.33 %
W Yorks
PAR-NEW-IN
1
69
0.11 %
0
0.00 %
65
94.20 %
5
7.25 %
4
5.80 %
0
0.00 %
England
283
40930
66.96 %
1745
4.26 %
35376
86.43 %
3151
7.70 %
2708
6.62 %
1101
2.69 %
Wales - High TB
Carms
PAR-IR-EX
5
17
0.03 %
8
47.06 %
9
52.94 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
14
102
0.17 %
42
41.18 %
53
51.96 %
7
6.86 %
7
6.86 %
0
0.00 %
PAR-PERSIS
6
126
0.21 %
0
0.00 %
110
87.30 %
14
11.11 %
12
9.52 %
4
3.17 %
PAR-RAPID
18
48
0.08 %
17
35.42 %
26
54.17 %
5
10.42 %
4
8.33 %
1
2.08 %
PAR-RECUR
3
243
0.40 %
19
7.82 %
210
86.42 %
14
5.76 %
13
5.35 %
1
0.41 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
5
471
0.77 %
32
6.79 %
414
87.90 %
28
5.94 %
22
4.67 %
3
0.64 %
Ceredigion
PAR-IR-EX
4
15
0.02 %
2
13.33 %
11
73.33 %
2
13.33 %
2
13.33 %
0
0.00 %
PAR-IR-SEV
6
10
0.02 %
6
60.00 %
3
30.00 %
1
10.00 %
1
10.00 %
0
0.00 %
PAR-RAPID
4
7
0.01 %
4
57.14 %
2
28.57 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-RECUR
3
147
0.24 %
6
4.08 %
137
93.20 %
3
2.04 %
3
2.04 %
1
0.68 %
SER-FLEXI
8
455
0.74 %
84
18.46 %
347
76.26 %
37
8.13 %
24
5.27 %
0
0.00 %
Gwent
PAR-IR-EX
1
8
0.01 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
16
0.03 %
9
56.25 %
6
37.50 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-RAPID
6
15
0.02 %
3
20.00 %
12
80.00 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
130
0.21 %
4
3.08 %
121
93.08 %
10
7.69 %
5
3.85 %
0
0.00 %
SER-FLEXI
1
110
0.18 %
4
3.64 %
104
94.55 %
4
3.64 %
2
1.82 %
0
0.00 %
Pembs
PAR-IR-EX
19
58
0.09 %
26
44.83 %
28
48.28 %
3
5.17 %
3
5.17 %
1
1.72 %
PAR-IR-SEV
45
305
0.50 %
110
36.07 %
169
55.41 %
31
10.16 %
15
4.92 %
11
3.61 %
PAR-OTHER
1
14
0.02 %
5
35.71 %
8
57.14 %
1
7.14 %
1
7.14 %
0
0.00 %
PAR-PERSIS
15
1,067
1.75 %
101
9.47 %
882
82.66 %
101
9.47 %
81
7.59 %
3
0.28 %
PAR-RAPID
28
98
0.16 %
24
24.49 %
67
68.37 %
9
9.18 %
6
6.12 %
1
1.02 %
PAR-RECUR
14
1,205
1.97 %
65
5.39 %
1043
86.56 %
91
7.55 %
72
5.98 %
25
2.07 %
SER-FLEXI
12
700
1.15 %
42
6.00 %
619
88.43 %
51
7.29 %
38
5.43 %
1
0.14 %
Powys
PAR-IR-EX
4
7
0.01 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-IR-SEV
12
88
0.14 %
14
15.91 %
64
72.73 %
13
14.77 %
9
10.23 %
1
1.14 %
PAR-NEW-IN
2
152
0.25 %
13
8.55 %
131
86.18 %
10
6.58 %
8
5.26 %
0
0.00 %
PAR-PERSIS
3
4
0.01 %
0
0.00 %
2
50.00 %
1
25.00 %
1
25.00 %
1
25.00 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
12
22
0.04 %
3
13.64 %
19
86.36 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
270
0.44 %
9
3.33 %
217
80.37 %
46
17.04 %
42
15.56 %
2
0.74 %
S Wales
PAR-IR-SEV
1
9
0.01 %
6
66.67 %
3
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
75
0.12 %
6
8.00 %
54
72.00 %
16
21.33 %
15
20.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.01 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
253
0.41 %
43
17.00 %
195
77.08 %
15
5.93 %
14
5.53 %
1
0.40 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
397
0.65 %
4
1.01 %
368
92.70 %
21
5.29 %
20
5.04 %
5
1.26 %
Ceredigion
PAR-IR-EX
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
341
0.56 %
45
13.20 %
271
79.47 %
29
8.50 %
25
7.33 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
1
306
0.50 %
1
0.33 %
293
95.75 %
15
4.90 %
11
3.59 %
1
0.33 %
Mid Glamorgan
PAR-RAPID
1
2
0.00 %
1
50.00 %
0
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
NE Wales
PAR-IR-EX
9
29
0.05 %
7
24.14 %
20
68.97 %
2
6.90 %
2
6.90 %
0
0.00 %
PAR-IR-SEV
7
60
0.10 %
10
16.67 %
49
81.67 %
1
1.67 %
1
1.67 %
0
0.00 %
PAR-NEW-IN
26
1,882
3.08 %
47
2.50 %
1664
88.42 %
172
9.14 %
156
8.29 %
15
0.80 %
PAR-OTHER
1
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
128
0.21 %
7
5.47 %
106
82.81 %
18
14.06 %
15
11.72 %
0
0.00 %
PAR-POSTM
12
65
0.11 %
6
9.23 %
56
86.15 %
4
6.15 %
3
4.62 %
0
0.00 %
PAR-RAPID
12
27
0.04 %
6
22.22 %
20
74.07 %
1
3.70 %
1
3.70 %
0
0.00 %
PAR-RECUR
1
56
0.09 %
1
1.79 %
50
89.29 %
6
10.71 %
5
8.93 %
0
0.00 %
SER-FLEXI
4
86
0.14 %
6
6.98 %
75
87.21 %
5
5.81 %
5
5.81 %
0
0.00 %
Powys
PAR-IR-EX
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
2
92
0.15 %
3
3.26 %
78
84.78 %
12
13.04 %
11
11.96 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
128
0.21 %
0
0.00 %
118
92.19 %
13
10.16 %
10
7.81 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-PERSIS
2
5
0.01 %
0
0.00 %
5
100.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
52
3,924
6.42 %
116
2.96 %
3543
90.29 %
293
7.47 %
248
6.32 %
17
0.43 %
PAR-OTHER
2
11
0.02 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
1
7
0.01 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
5
5
0.01 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
291
0.48 %
10
3.44 %
267
91.75 %
18
6.19 %
13
4.47 %
1
0.34 %
NE Wales
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
34
3,084
5.05 %
70
2.27 %
2591
84.01 %
376
12.19 %
197
6.39 %
226
7.33 %
PAR-POSTM
9
23
0.04 %
3
13.04 %
20
86.96 %
1
4.35 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
4
165
0.27 %
12
7.27 %
138
83.64 %
17
10.30 %
15
9.09 %
0
0.00 %
NW Wales
PAR-NEW-IN
2
24
0.04 %
0
0.00 %
23
95.83 %
1
4.17 %
1
4.17 %
0
0.00 %
Wales
494
17425
28.51 %
1080
6.20 %
14874
85.36 %
1531
8.79 %
1149
6.59 %
322
1.85 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
7
768
1.26 %
75
9.77 %
592
77.08 %
108
14.06 %
96
12.50 %
5
0.65 %
West lothian
PAR-NEW-IN
9
797
1.30 %
31
3.89 %
640
80.30 %
61
7.65 %
58
7.28 %
68
8.53 %
Wigtown
PAR-NEW-IN
19
1,208
1.98 %
110
9.11 %
994
82.28 %
108
8.94 %
103
8.53 %
1
0.08 %
Scotland
35
2773
4.54 %
216
7.79 %
2226
80.27 %
277
9.99 %
257
9.27 %
74
2.67 %
Grand Total
Sum:
812
61128
3041
52476
4959
4114
1497
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol March 2023
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
103
14014
100.0%
615
4.4%
12507
89.2%
1033
7.4%
885
6.3%
7
0.0%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
103
14014
100.0%
615
4.4%
12507
89.2%
1033
7.4%
885
6.3%
7
0.0%
Scotland
Parallel
14
1185
100.0%
59
5.0%
1003
84.6%
125
10.5%
112
9.5%
11
0.9%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
14
1185
100.0%
59
5.0%
1003
84.6%
125
10.5%
112
9.5%
11
0.9%
Wales
Parallel
141
4398
93.7%
179
4.1%
3941
89.6%
294
6.7%
253
5.8%
25
0.6%
Serial/Extended
8
297
6.3%
14
4.7%
264
88.9%
24
8.1%
18
6.1%
1
0.3%
Total
149
4695
100.0%
193
4.1%
4205
89.6%
318
6.8%
271
5.8%
26
0.6%
GB
Parallel
258
19597
98.5%
853
4.4%
17451
89.0%
1452
7.4%
1250
6.4%
43
0.2%
Serial/Extended
8
297
1.5%
14
4.7%
264
88.9%
24
8.1%
18
6.1%
1
0.3%
Total
266
19894
100.0%
867
4.4%
17715
89.0%
1476
7.4%
1268
6.4%
44
0.2%
103.00
149.00
14.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2023)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
283
40930
100.0%
1745
4.3%
35376
86.4%
3151
7.7%
2708
6.6%
1101
2.7%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
283
40930
100.0%
1745
4.3%
35376
86.4%
3151
7.7%
2708
6.6%
1101
2.7%
Scotland
Parallel
35
2773
100.0%
216
7.8%
2226
80.3%
277
10.0%
257
9.3%
74
2.7%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
35
2773
100.0%
216
7.8%
2226
80.3%
277
10.0%
257
9.3%
74
2.7%
Wales
Parallel
460
15438
88.6%
900
5.8%
13177
85.4%
1389
9.0%
1043
6.8%
318
2.1%
Serial/Extended
34
1987
11.4%
180
9.1%
xx-xxxx-xxxx
85.4%
142
7.1%
106
5.3%
4
0.2%
Total
494
17425
100.0%
1080
6.2%
14874
85.4%
1531
8.8%
1149
6.6%
322
1.8%
GB
Parallel
778
59141
96.7%
2861
4.8%
50779
85.9%
4817
8.1%
4008
6.8%
1493
2.5%
Serial/Extended
34
1987
3.3%
180
9.1%
xx-xxxx-xxxx
85.4%
142
7.1%
106
5.3%
4
0.2%
Total
812
61128
100.0%
3041
5.0%
52476
85.8%
4959
8.1%
4114
6.7%
1497
2.4%
283.00
494.00
35.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
8
297
1.5%
14
4.7%
264
88.9%
24
8.1 %
18
6.1 %
1
0.3 %
Parallel - Herd or Group Slaughter
2
396
2.0%
35
8.8%
326
82.3%
48
12.1 %
35
8.8 %
0
Parallel - IRs in Exit Strategy for persistent her
12
37
0.2%
9
24.3%
25
67.6%
3
8.1 %
3
8.1 %
0
Parallel New Infection
89
7977
40.1%
203
2.5%
7297
91.5%
511
6.4 %
444
5.6 %
33
0.4 %
Parallel - new infection cull area
6
1019
5.1%
49
4.8%
914
89.7%
72
7.1 %
56
5.5 %
0
Parallel - Other
9
465
2.3%
31
6.7%
395
84.9%
43
9.2 %
39
8.4 %
0
Parallel Persistent Infection
14
1147
5.8%
111
9.7%
925
80.6%
119
10.4 %
111
9.7 %
0
Parallel - persistent infection cull area
1
539
2.7%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX an
11
60
0.3%
5
8.3%
55
91.7%
1
1.7 %
0
0
Parallel - Rapid Testing Two-times IRs
27
78
0.4%
12
15.4%
59
75.6%
9
11.5 %
7
9.0 %
0
Parallel - Recurrent breakdowns
59
7716
38.8%
324
4.2%
6858
88.9%
609
7.9 %
525
6.8 %
9
0.1 %
Parallel - severe IRs - in persistent herds
28
163
0.8%
43
26.4%
116
71.2%
5
3.1 %
4
2.5 %
0
Total
266
19894
100.0%
867
4.4%
17715
89.0%
1476
7.4 %
1268
6.4 %
44
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (March 2023)
England - HRA
Parallel - Herd or Group Slaughter
2
396
2.0%
35
8.8%
326
82.3%
48
12.1 %
35
8.8 %
0
Parallel - new infection cull area
5
828
4.2%
46
5.6%
732
88.4%
66
8.0 %
50
6.0 %
0
Parallel - Other
6
439
2.2%
28
6.4%
379
86.3%
36
8.2 %
32
7.3 %
0
Parallel Persistent Infection
3
377
1.9%
50
13.3%
280
74.3%
49
13.0 %
47
12.5 %
0
Parallel - persistent infection cull area
1
539
2.7%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel - Recurrent breakdowns
41
6387
32.1%
277
4.3%
5681
88.9%
499
7.8 %
425
6.7 %
4
0.1 %
England - HRA
58
8966
45.1%
467
5.2%
7879
87.9%
730
8.1 %
615
6.9 %
5
0.1 %
England - Edge
Parallel New Infection
29
3555
17.9%
99
2.8%
3319
93.4%
159
4.5 %
135
3.8 %
2
0.1 %
Parallel - new infection cull area
1
191
1.0%
3
1.6%
182
95.3%
6
3.1 %
6
3.1 %
0
Parallel - Other
1
5
0.0%
0
0.0 %
1
20.0%
4
80.0 %
4
80.0 %
0
Parallel Persistent Infection
1
235
1.2%
14
6.0%
205
87.2%
16
6.8 %
16
6.8 %
0
Parallel - Recurrent breakdowns
5
326
1.6%
17
5.2%
279
85.6%
33
10.1 %
30
9.2 %
0
England - Edge
37
4312
21.7%
133
3.1%
3986
92.4%
218
5.1 %
191
4.4 %
2
0.0 %
England - LRA
Parallel New Infection
8
736
3.7%
15
2.0%
642
87.2%
85
11.5 %
79
10.7 %
0
England - LRA
8
736
3.7%
15
2.0%
642
87.2%
85
11.5 %
79
10.7 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
7
295
1.5%
14
4.7%
263
89.2%
23
7.8 %
17
5.8 %
1
0.3 %
Parallel - IRs in Exit Strategy for persistent her
9
32
0.2%
7
21.9%
22
68.8%
3
9.4 %
3
9.4 %
0
Parallel - Other
1
12
0.1%
1
8.3%
8
66.7%
3
25.0 %
3
25.0 %
0
Parallel Persistent Infection
7
419
2.1%
37
8.8%
345
82.3%
43
10.3 %
37
8.8 %
0
Parallel - Rapid Testing Two-times IRs
20
57
0.3%
9
15.8%
43
75.4%
7
12.3 %
5
8.8 %
0
Parallel - Recurrent breakdowns
12
997
5.0%
30
3.0%
893
89.6%
76
7.6 %
69
6.9 %
5
0.5 %
Parallel - severe IRs - in persistent herds
21
132
0.7%
38
28.8%
90
68.2%
5
3.8 %
4
3.0 %
0
Wales - High TB
77
1944
9.8%
136
7.0%
1664
85.6%
160
8.2 %
138
7.1 %
6
0.3 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel - IRs in Exit Strategy for persistent her
3
5
0.0%
2
40.0%
3
60.0%
0
0
0
Parallel New Infection
12
825
4.1%
5
0.6%
773
93.7%
50
6.1 %
44
5.3 %
3
0.4 %
Parallel - Other
1
9
0.0%
2
22.2%
7
77.8%
0
0
0
Parallel Persistent Infection
3
116
0.6%
10
8.6%
95
81.9%
11
9.5 %
11
9.5 %
0
Parallel – post-mvt test coupled with IDEXX an
4
40
0.2%
3
7.5%
37
92.5%
0
0
0
Parallel - Rapid Testing Two-times IRs
7
21
0.1%
3
14.3%
16
76.2%
2
9.5 %
2
9.5 %
0
Parallel - Recurrent breakdowns
1
6
0.0%
0
0.0 %
5
83.3%
1
16.7 %
1
16.7 %
0
Parallel - severe IRs - in persistent herds
5
29
0.1%
4
13.8%
25
86.2%
0
0
0
Wales - Intermediate TB
37
1053
5.3%
29
2.8%
962
91.4%
65
6.2 %
59
5.6 %
3
0.3 %
Wales - Low TB
Parallel New Infection
26
1676
8.4%
25
1.5%
1560
93.1%
92
5.5 %
74
4.4 %
17
1.0 %
Parallel – post-mvt test coupled with IDEXX an
7
20
0.1%
2
10.0%
18
90.0%
1
5.0 %
0
0
Parallel - severe IRs - in persistent herds
2
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales - Low TB
35
xx-xxxx-xxxx
8.5%
28
1.6%
1579
93.0%
93
5.5 %
74
4.4 %
17
1.0 %
Scotland - Scotland
Parallel New Infection
14
1185
6.0%
59
5.0%
1003
84.6%
125
10.5 %
112
9.5 %
11
0.9 %
Scotland - Scotland
14
1185
6.0%
59
5.0%
1003
84.6%
125
10.5 %
112
9.5 %
11
0.9 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
34
1987
3.3%
180
9.1%
xx-xxxx-xxxx
85.4%
142
7.1 %
106
5.3 %
4
0.2 %
Parallel - Herd or Group Slaughter
2
396
0.6%
35
8.8%
326
82.3%
48
12.1 %
35
8.8 %
0
Parallel - IRs in Exit Strategy for persistent herds
45
139
0.2%
50
36.0%
79
56.8%
10
7.2 %
9
6.5 %
1
0.7 %
Parallel New Infection
246
23714
38.8%
781
3.3%
20655
87.1%
1932
8.1 %
1547
6.5 %
731
3.1 %
Parallel - new infection cull area
9
1830
3.0%
109
6.0%
1607
87.8%
140
7.7 %
114
6.2 %
0
Parallel - Other
20
1337
2.2%
101
7.6%
1140
85.3%
82
6.1 %
76
5.7 %
20
1.5 %
Parallel Persistent Infection
48
3518
5.8%
249
7.1%
2948
83.8%
358
10.2 %
313
8.9 %
8
0.2 %
Parallel - persistent infection cull area
1
539
0.9%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX anti
27
94
0.2%
11
11.7%
80
85.1%
5
5.3 %
3
3.2 %
0
Parallel - Rapid Testing Two-times IRs
90
234
0.4%
59
25.2%
159
67.9%
19
8.1 %
14
6.0 %
2
0.9 %
Parallel - Recurrent breakdowns
197
26741
43.7%
1233
4.6%
22953
85.8%
2137
8.0 %
1837
6.9 %
718
2.7 %
Parallel - severe IRs - in persistent herds
93
599
1.0%
202
33.7%
351
58.6%
54
9.0 %
34
5.7 %
12
2.0 %
Total
812
61128
100.0%
3041
5.0%
52476
85.8%
4959
8.1 %
4114
6.7 %
1497
2.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2023)
England - HRA
Parallel - Herd or Group Slaughter
2
396
0.6%
35
8.8%
326
82.3%
48
12.1 %
35
8.8 %
0
Parallel - new infection cull area
8
1639
2.7%
106
6.5%
1425
86.9%
134
8.2 %
108
6.6 %
0
Parallel - Other
6
439
0.7%
28
6.4%
379
86.3%
36
8.2 %
32
7.3 %
0
Parallel Persistent Infection
3
377
0.6%
50
13.3%
280
74.3%
49
13.0 %
47
12.5 %
0
Parallel - persistent infection cull area
1
539
0.9%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel - Recurrent breakdowns
133
21992
36.0%
1046
4.8%
18768
85.3%
1753
8.0 %
1510
6.9 %
668
3.0 %
England - Edge
Parallel New Infection
65
7780
12.7%
217
2.8%
7036
90.4%
492
6.3 %
404
5.2 %
123
1.6 %
Parallel - new infection cull area
1
191
0.3%
3
1.6%
182
95.3%
6
3.1 %
6
3.1 %
0
Parallel - Other
4
535
0.9%
16
3.0%
489
91.4%
11
2.1 %
11
2.1 %
19
3.6 %
Parallel Persistent Infection
9
1422
2.3%
46
3.2%
1245
87.6%
143
10.1 %
131
9.2 %
0
Parallel - Recurrent breakdowns
23
1882
3.1%
69
3.7%
1654
87.9%
162
8.6 %
144
7.7 %
15
0.8 %
England - LRA
Parallel New Infection
27
3697
6.0%
98
2.7%
3070
83.0%
284
7.7 %
254
6.9 %
275
7.4 %
Parallel Persistent Infection
1
41
0.1%
0
0.0 %
41
100.0%
1
2.4 %
0
0
England
283
40930
67.0%
1745
4.3%
35376
86.4%
3151
7.7 %
2708
6.6 %
1101
2.7 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Wales - High TB
Extended Flexible IFN-GAMMA test
26
1736
2.8%
162
9.3%
1484
85.5%
120
6.9 %
86
5.0 %
4
0.2 %
Parallel - IRs in Exit Strategy for persistent herd
33
105
0.2%
40
38.1%
57
54.3%
7
6.7 %
7
6.7 %
1
1.0 %
Parallel New Infection
2
152
0.2%
13
8.6%
131
86.2%
10
6.6 %
8
5.3 %
0
Parallel - Other
3
89
0.1%
11
12.4%
62
69.7%
17
19.1 %
16
18.0 %
0
Parallel Persistent Infection
24
1197
2.0%
101
8.4%
994
83.0%
116
9.7 %
94
7.9 %
8
0.7 %
Parallel – post-mvt test coupled with IDEXX ant
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
69
191
0.3%
52
27.2%
126
66.0%
16
8.4 %
11
5.8 %
2
1.0 %
Parallel - Recurrent breakdowns
27
1995
3.3%
103
5.2%
1728
86.6%
164
8.2 %
135
6.8 %
29
1.5 %
Parallel - severe IRs - in persistent herds
80
530
0.9%
187
35.3%
298
56.2%
53
10.0 %
33
6.2 %
12
2.3 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
86
0.1%
6
7.0%
75
87.2%
5
5.8 %
5
5.8 %
0
Parallel - IRs in Exit Strategy for persistent herd
11
33
0.1%
9
27.3%
22
66.7%
2
6.1 %
2
6.1 %
0
Parallel New Infection
29
2280
3.7%
51
2.2%
2035
89.3%
199
8.7 %
178
7.8 %
16
0.7 %
Parallel - Other
5
263
0.4%
45
17.1%
203
77.2%
15
5.7 %
14
5.3 %
1
0.4 %
Parallel Persistent Infection
10
474
0.8%
52
11.0%
382
80.6%
48
10.1 %
40
8.4 %
0
Parallel – post-mvt test coupled with IDEXX ant
12
65
0.1%
6
9.2%
56
86.2%
4
6.2 %
3
4.6 %
0
Parallel - Rapid Testing Two-times IRs
16
33
0.1%
7
21.2%
24
72.7%
2
6.1 %
2
6.1 %
0
Parallel - Recurrent breakdowns
9
581
1.0%
5
0.9%
536
92.3%
40
6.9 %
35
6.0 %
5
0.9 %
Parallel - severe IRs - in persistent herds
10
66
0.1%
14
21.2%
51
77.3%
1
1.5 %
1
1.5 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
165
0.3%
12
7.3%
138
83.6%
17
10.3 %
15
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
1
1
0.0%
1
100.0%
0
0.0 %
1
100.0 %
0
0
Parallel New Infection
88
7032
11.5%
186
2.6%
6157
87.6%
670
9.5 %
446
6.3 %
243
3.5 %
Parallel - Other
2
11
0.0%
1
9.1%
7
63.6%
3
27.3 %
3
27.3 %
0
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX ant
14
28
0.0%
5
17.9%
23
82.1%
1
3.6 %
0
0
Parallel - Rapid Testing Two-times IRs
5
10
0.0%
0
0.0 %
9
90.0%
1
10.0 %
1
10.0 %
0
Parallel - Recurrent breakdowns
5
291
0.5%
10
3.4%
267
91.8%
18
6.2 %
13
4.5 %
1
0.3 %
Parallel - severe IRs - in persistent herds
3
3
0.0%
1
33.3%
2
66.7%
0
0
0
Wales
494
17425
28.5%
1080
6.2%
14874
85.4%
1531
8.8 %
1149
6.6 %
322
1.8 %
Scotland - Scotland
Parallel New Infection
35
2773
4.5%
216
7.8%
2226
80.3%
277
10.0 %
257
9.3 %
74
2.7 %
Scotland
35
2773
4.5%
216
7.8%
2226
80.3%
277
10.0 %
257
9.3 %
74
2.7 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2022 April
220
15067
130
687
9
0
0
0
0
50
2
0
11
0
563
0
52
0
0
May
269
23949
158
918
2
19
0
0
0
0
62
3
0
9
0
778
0
44
1
0
June
213
17755
114
590
19
0
0
0
0
48
1
0
2
0
460
1
57
2
0
July
248
15402
109
485
4
0
0
0
0
46
1
2
3
0
382
1
46
0
0
August
274
19758
144
758
12
0
0
0
0
36
3
0
14
0
642
0
47
1
2
September
205
xx-xxxx-xxxx
107
672
13
0
0
0
0
100
0
1
7
0
520
0
31
0
0
October
180
xx-xxxx-xxxx
100
620
12
0
0
0
0
66
0
0
21
0
495
0
26
0
0
November
201
15848
120
673
16
0
0
0
0
60
1
1
13
0
504
1
77
0
0
December
200
13735
109
545
36
0
0
0
0
26
0
0
10
1
426
0
41
0
0
2023 January
281
23696
176
1138
66
0
0
0
0
81
1
0
14
5
868
3
75
1
24
February
265
17538
147
1036
17
0
0
0
0
69
4
2
7
19
830
3
11
0
74
March
266
19894
159
867
194
311
0
0
0
0
30
0
5
0
1
184
7
0
0
6
Totals (last
12 months)
2822
215482
1573
8989
196
534
0
0
0
0
674
16
11
111
26
6652
16
507
5
106
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1045
146077
710
5259
397
0
0
0
0
549
3
3
22
3
4032
1
18
0
0
Scotland
69
7197
46
342
7
0
0
0
0
94
4
4
17
6
165
2
19
0
0
Wales
1708
62208
817
3388
130
0
0
0
0
31
9
4
72
17
2455
13
470
5
106
Totals
2822
215482
1573
8989
534
0
0
0
0
674
16
11
111
26
6652
16
507
5
106
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2021
2022
2023
IFNg Positive herds
IFNg Negative herds
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2021
2022
2023
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2021
2022
2023
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
Wiltshire
W Glamorgan
Warks
Somerset excl N
Powys
Oxon
NW Wales
NE Wales
Lincs
Heref
Gwynedd
Gloucs
Devon
Cumbria
Cheshire
Carms
Ayrshire
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during March 2023 (% submission per
County)
County
%Resampled %Retested
Ayrshire
17.11 %
19.46 %
Bucks
2.74 %
2.86 %
Carms
5.52 %
6.90 %
Ceredigion
6.47 %
6.97 %
Cheshire
4.82 %
6.11 %
Corn & Scilly
7.67 %
8.94 %
Cumbria
10.05 %
10.55 %
Derbyshire
7.27 %
10.91 %
Devon
5.43 %
6.95 %
E Sussex
5.46 %
6.56 %
Gloucs
42.86 %
42.86 %
Gwent
1.71 %
3.42 %
Gwynedd
4.10 %
5.17 %
Hampshire
5.14 %
5.87 %
Heref
4.88 %
5.73 %
Leics & Rut
3.32 %
3.65 %
Lincs
11.52 %
12.32 %
Mid Glamorgan
50.00 %
50.00 %
NE Wales
6.21 %
6.75 %
Notts
33.33 %
33.33 %
NW Wales
N Yorks
4.76 %
7.14 %
Oxon
8.20 %
8.74 %
Pembs
6.31 %
7.39 %
Powys
14.86 %
16.06 %
Shropshire
13.62 %
15.79 %
Somerset excl
6.39 %
7.21 %
Staffs
7.87 %
8.73 %
Warks
3.26 %
4.60 %
West lothian
6.13 %
6.69 %
W Glamorgan
25.00 %
25.00 %
Wigtown
7.39 %
8.14 %
Wiltshire
7.95 %
11.36 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
13783
615
4.5%
14014
615
4.4%
Wales
4634
193
4.2%
4695
193
4.1%
Scotland
1091
59
5.4%
1185
59
5.0%
GB
19508
867
4.4%
19894
867
4.4%
Table 6a (i): Number of animals and submissions tested in March 2023
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
38874
1745
4.5%
40930
1745
4.3%
Wales
16361
1080
6.6%
17425
1080
6.2%
Scotland
1734
216
12.5%
2773
216
7.8%
GB
56966
3041
5.3%
61128
3041
5.0%
Table 6b (i): Number of animals and submissions tested in 2023 Up to end of March
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
18807
801
4.3%
19262
801
4.2%
Wales
3655
307
8.4%
3781
307
8.1%
Scotland
3
0
0.0 %
3
0.0 %
GB
22465
1108
4.9%
23046
1108
4.8%
Table 6a (ii): Number of animals and submissions tested in March 2022
Table 6b (ii): Number of animals and submissions tested in 2022 Up to end of March
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
46786
2036
4.4%
48739
2036
4.2%
Wales
10463
890
8.5%
11063
890
8.0%
Scotland
5
0
0.0 %
5
0.0 %
GB
57254
2926
5.1%
59807
2926
4.9%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cumbria
PRV-SE
1
3
3
100.00 %
Devon
PRV-SE
1
13
6
46.15 %
West Sussex
PRV-SE
1
1
0
0
PRV-SP
1
5
0
0
Total
4
22
9
40.91 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples March 2023
Table 7b Number of private samples 2023
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
2
0
0
Cumbria
PRV-SE
1
3
3
100.00 %
Devon
PRV-SE
4
99
10
10.10 %
Oxfordshire
PRV-SP
2
2
0
0
Shropshire
PRV-SP
2
2
0
0
West Sussex
PRV-SE
1
1
0
0
PRV-SP
1
5
0
0
Total
12
114
13
11.40 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
569
38
6.7%
England
2
569
38
6.7%
Wales
Carmarthenshire
BOV-IR-C
5
21
5
23.8%
BOV-IR-EX
1
6
0
0
Ceredigion
BOV-IR-C
1
1
0
0
North East Wales
BOV-IR-C
4
24
7
29.2%
BOV-OTHER
1
54
0
0
BOV-POSTM
1
1
1
100.0%
Pembrokeshire
BOV-IR-C
11
114
27
23.7%
BOV-IR-EX
2
12
1
8.3%
Powys
BOV-IR-C
1
1
1
100.0%
South Wales
BOV-IR-C
1
3
1
33.3%
BOV-OTHER
1
30
1
3.3%
BOV-P
1
80
3
3.8%
Wales
30
347
47
13.5%
All GB
32
916
85
9.3 %
Table 8a Number of Cattle Antibody Tests March 2023
Table 8b Number of Cattle Antibody Tests 2023
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
569
38
6.7%
Northern Somerset &
South
Gloucestershire
BOV-P
1
5
0
0
England
3
574
38
6.6%
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
11
111
30
27.0%
BOV-IR-EX
5
17
2
11.8%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
7
47
10
21.3%
BOV-IR-EX
1
3
0
0
BOV-OTHER
1
80
2
2.5%
North East Wales
BOV-IR-C
6
37
9
24.3%
BOV-IR-EX
2
11
0
0
BOV-OTHER
1
54
0
0
BOV-P
1
14
0
0
BOV-POSTM
8
21
5
23.8%
Pembrokeshire
BOV-IR-C
33
345
52
15.1%
BOV-IR-EX
8
52
7
13.5%
BOV-OTHER
3
328
6
1.8%
Powys
1
8
1
12.5%
BOV-IR-C
13
91
12
13.2%
South Wales
BOV-IR-C
3
26
6
23.1%
BOV-IR-EX
1
8
2
25.0%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-OTHER
1
30
1
3.3%
BOV-P
1
80
3
3.8%
Wales
108
1,412
149
10.6%
All GB
112
1,988
188
9.5 %