Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - February 2022
Source: environment.data.gov.uk (2023-01-25)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2022. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 186
Report Period 1st - 28th February 2022
Issued 10 March 2022

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
February
2022
%
Total 2022
%
Submissions
229
491
Samples
Total (%)
18198
100.00 %
36761
100.00 %
England
14403
79.15 %
29477
80.19 %
Wales
3795
20.85 %
7282
19.81 %
Scotland
2
0.01 %
Parallel Tests
PAR-CHR
576
3.17 %
576
1.57 %
PAR-CUL-N
2353
12.93 %
4742
12.90 %
PAR-CUL-P
28
0.15 %
1770
4.81 %
PAR-HERD-S
6
0.03 %
40
0.11 %
PAR-IR-EX
33
0.18 %
56
0.15 %
PAR-IR-SEV
117
0.64 %
245
0.67 %
PAR-NEW-IN
3108
17.08 %
5876
15.98 %
PAR-OTHER
548
3.01 %
1139
3.10 %
PAR-PERSIS
xx-xxxx-xxxx
9.04 %
5154
14.02 %
PAR-POSTM
17
0.09 %
74
0.20 %
PAR-RAPID
100
0.55 %
198
0.54 %
PAR-RECUR
7372
40.51 %
13196
35.90 %
Total(% of all samples)
15904
87.39 %
33066
89.95 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
2294
12.61 %
3692
10.04 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
1
0.00 %
SER-OTHER
0
0.00 %
2
0.01 %
Total(% of all samples)
2294
12.61 %
3695
10.05 %
Total
18198
100.00 %
36761
100.00 %
Retests
Total (% of all samples)
1626
8.94 %
3021
8.22 %
England
1321
7.26 %
2445
6.65 %
Wales
305
1.68 %
576
1.57 %
Scotland
Resamples
Total (% of all samples)
1396
7.67 %
2635
7.17 %
England
1148
6.31 %
2151
5.85 %
Wales
248
1.36 %
484
1.32 %
Scotland
Rejects
Total (% of all samples)
38
0.21 %
443
1.21 %
England
32
0.18 %
362
0.98 %
Wales
6
0.03 %
81
0.22 %
Scotland
0
0.00 %
Table 1. Monthly Totals for February 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR IR EX
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CHR
576
3.2 %
PAR-CUL-N
2353
12.9 %
PAR-CUL-P
28
0.2 %
PAR-HERD-S
6
0.0 %
PAR-IR-EX
33
0.2 %
PAR-IR-SEV
117
0.6 %
PAR-NEW-IN
3108
17.1 %
PAR-OTHER
548
3.0 %
PAR-PERSIS
xx-xxxx-xxxx
9.0 %
PAR-POSTM
17
0.1 %
PAR-RAPID
100
0.5 %
PAR-RECUR
7372
40.5 %
SER-FLEXI
2294
12.6 %
Total
18198
100.00 %
Figure 1. Number of samples submitted in each category during February 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
W Glamorgan
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Ceredigion
Carms
W Yorks
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northants
Leics & Rut
Herts
Heref
Gloucs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Avon
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during February 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
1000
2000
3000
4000
5000
6000
Argyll & Bute
W Glamorgan
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northumb
Northants
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
1
14
0.08 %
1
7.14 %
11
78.57 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-RECUR
2
140
0.77 %
4
2.86 %
124
88.57 %
13
9.29 %
12
8.57 %
0
0.00 %
Corn & Scilly
PAR-CUL-P
1
26
0.14 %
2
7.69 %
16
61.54 %
8
30.77 %
8
30.77 %
0
0.00 %
PAR-PERSIS
2
27
0.15 %
1
3.70 %
15
55.56 %
11
40.74 %
11
40.74 %
0
0.00 %
PAR-RECUR
10
1,773
9.74 %
64
3.61 %
1546
87.20 %
194
10.94 %
162
9.14 %
1
0.06 %
Devon
PAR-CUL-N
2
322
1.77 %
26
8.07 %
255
79.19 %
50
15.53 %
41
12.73 %
0
0.00 %
PAR-CUL-P
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
4
644
3.54 %
50
7.76 %
551
85.56 %
66
10.25 %
43
6.68 %
0
0.00 %
PAR-RECUR
14
1,597
8.78 %
150
9.39 %
1327
83.09 %
131
8.20 %
118
7.39 %
2
0.13 %
Dorset
PAR-CUL-N
5
1,423
7.82 %
58
4.08 %
1279
89.88 %
100
7.03 %
83
5.83 %
3
0.21 %
PAR-OTHER
2
49
0.27 %
1
2.04 %
47
95.92 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-RECUR
1
432
2.37 %
7
1.62 %
399
92.36 %
28
6.48 %
26
6.02 %
0
0.00 %
Gloucs
PAR-RECUR
6
1,793
9.85 %
36
2.01 %
1664
92.81 %
106
5.91 %
92
5.13 %
1
0.06 %
Heref
PAR-OTHER
2
408
2.24 %
22
5.39 %
372
91.18 %
24
5.88 %
14
3.43 %
0
0.00 %
PAR-PERSIS
5
752
4.13 %
39
5.19 %
642
85.37 %
73
9.71 %
68
9.04 %
3
0.40 %
PAR-RECUR
1
6
0.03 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-CHR
2
576
3.17 %
6
1.04 %
536
93.06 %
38
6.60 %
34
5.90 %
0
0.00 %
PAR-RECUR
11
721
3.96 %
12
1.66 %
666
92.37 %
49
6.80 %
42
5.83 %
1
0.14 %
Somerset excl N
PAR-OTHER
2
83
0.46 %
15
18.07 %
63
75.90 %
6
7.23 %
5
6.02 %
0
0.00 %
PAR-RECUR
3
196
1.08 %
20
10.20 %
168
85.71 %
9
4.59 %
8
4.08 %
0
0.00 %
Staffs
PAR-CUL-N
1
256
1.41 %
6
2.34 %
207
80.86 %
43
xx-xxxx-xxxx %
43
xx-xxxx-xxxx %
0
0.00 %
PAR-PERSIS
3
218
1.20 %
1
0.46 %
189
86.70 %
28
12.84 %
28
12.84 %
0
0.00 %
PAR-RECUR
3
321
1.76 %
16
4.98 %
289
90.03 %
23
7.17 %
16
4.98 %
0
0.00 %
Wiltshire
PAR-CUL-N
2
310
1.70 %
20
6.45 %
266
85.81 %
26
8.39 %
24
7.74 %
0
0.00 %
PAR-CUL-P
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Cheshire
PAR-CUL-N
1
28
0.15 %
1
3.57 %
20
71.43 %
7
25.00 %
7
25.00 %
0
0.00 %
Table 2a. Summary by County for February 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
1
15
0.08 %
0
0.00 %
10
66.67 %
5
33.33 %
5
33.33 %
0
0.00 %
PAR-PERSIS
1
5
0.03 %
0
0.00 %
3
60.00 %
3
60.00 %
2
40.00 %
0
0.00 %
PAR-RECUR
1
98
0.54 %
5
5.10 %
88
89.80 %
6
6.12 %
5
5.10 %
0
0.00 %
Derbyshire
PAR-NEW-IN
1
300
1.65 %
10
3.33 %
257
85.67 %
34
11.33 %
31
10.33 %
2
0.67 %
Leics & Rut
PAR-NEW-IN
2
151
0.83 %
5
3.31 %
130
86.09 %
20
13.25 %
16
10.60 %
0
0.00 %
Northants
PAR-NEW-IN
6
583
3.20 %
6
1.03 %
507
86.96 %
73
12.52 %
70
12.01 %
0
0.00 %
Oxon
PAR-NEW-IN
4
432
2.37 %
23
5.32 %
366
84.72 %
48
11.11 %
43
9.95 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
212
1.16 %
2
0.94 %
174
82.08 %
21
9.91 %
18
8.49 %
18
8.49 %
Herts
PAR-NEW-IN
3
250
1.37 %
14
5.60 %
196
78.40 %
41
xx-xxxx-xxxx %
39
15.60 %
1
0.40 %
N Yorks
PAR-NEW-IN
1
151
0.83 %
1
0.66 %
139
92.05 %
11
7.28 %
11
7.28 %
0
0.00 %
W Yorks
PAR-NEW-IN
1
89
0.49 %
2
2.25 %
67
75.28 %
23
25.84 %
20
22.47 %
0
0.00 %
England
110
14403
79.15 %
626
4.35 %
12597
87.46 %
1321
9.17 %
1148
7.97 %
32
0.22 %
Wales - High TB
Carms
PAR-IR-SEV
2
3
0.02 %
0
0.00 %
3
100.00 %
1
33.33 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
14
0.08 %
4
28.57 %
9
64.29 %
1
7.14 %
1
7.14 %
0
0.00 %
SER-FLEXI
6
1,335
7.34 %
74
5.54 %
1197
89.66 %
74
5.54 %
61
4.57 %
3
0.22 %
Ceredigion
PAR-IR-SEV
3
15
0.08 %
8
53.33 %
6
40.00 %
1
6.67 %
1
6.67 %
0
0.00 %
PAR-RAPID
3
5
0.03 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
5
23
0.13 %
9
39.13 %
13
56.52 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-IR-SEV
9
91
0.50 %
32
35.16 %
55
60.44 %
4
4.40 %
4
4.40 %
0
0.00 %
PAR-RAPID
22
47
0.26 %
15
31.91 %
30
63.83 %
2
4.26 %
2
4.26 %
0
0.00 %
PAR-RECUR
3
266
1.46 %
8
3.01 %
238
89.47 %
30
11.28 %
19
7.14 %
1
0.38 %
SER-FLEXI
10
959
5.27 %
117
12.20 %
776
80.92 %
77
8.03 %
64
6.67 %
2
0.21 %
Powys
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
7
0.04 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-POSTM
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
7
16
0.09 %
4
25.00 %
12
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
5
0.03 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
NE Wales
PAR-IR-EX
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
5
298
1.64 %
3
1.01 %
283
94.97 %
13
4.36 %
12
4.03 %
0
0.00 %
PAR-POSTM
3
5
0.03 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-RAPID
2
7
0.04 %
4
57.14 %
3
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
273
1.50 %
17
6.23 %
238
87.18 %
22
8.06 %
18
6.59 %
0
0.00 %
S Glamorgan
PAR-OTHER
2
8
0.04 %
2
25.00 %
5
62.50 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-RAPID
1
3
0.02 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
23
0.13 %
7
30.43 %
16
69.57 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-IR-EX
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
1
6
0.03 %
1
16.67 %
2
33.33 %
3
50.00 %
3
50.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
347
1.91 %
23
6.63 %
270
77.81 %
66
19.02 %
54
15.56 %
0
0.00 %
NE Wales
PAR-HERD-S
1
6
0.03 %
2
33.33 %
2
33.33 %
2
33.33 %
2
33.33 %
0
0.00 %
PAR-NEW-IN
2
7
0.04 %
0
0.00 %
7
100.00 %
1
14.29 %
0
0.00 %
0
0.00 %
PAR-POSTM
6
10
0.05 %
1
10.00 %
8
80.00 %
2
20.00 %
1
10.00 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
119
3795
20.85 %
340
8.96 %
3201
84.35 %
305
8.04 %
248
6.53 %
6
0.16 %
Grand Total
Sum:
229
18198
966
15798
1626
1396
38
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
3
527
1.43 %
26
4.93 %
485
92.03 %
16
3.04 %
15
2.85 %
1
0.19 %
PAR-RECUR
5
411
1.12 %
8
1.95 %
370
90.02 %
36
8.76 %
31
7.54 %
2
0.49 %
Corn & Scilly
PAR-CUL-N
1
2
0.01 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-CUL-P
1
26
0.07 %
2
7.69 %
16
61.54 %
8
30.77 %
8
30.77 %
0
0.00 %
PAR-PERSIS
8
1,683
4.58 %
73
4.34 %
1491
88.59 %
137
8.14 %
119
7.07 %
0
0.00 %
PAR-RECUR
14
1,822
4.96 %
65
3.57 %
1575
86.44 %
201
11.03 %
181
9.93 %
1
0.05 %
Devon
PAR-CUL-N
5
964
2.62 %
59
6.12 %
812
84.23 %
106
11.00 %
92
9.54 %
1
0.10 %
PAR-CUL-P
3
412
1.12 %
8
1.94 %
383
92.96 %
28
6.80 %
19
4.61 %
2
0.49 %
PAR-PERSIS
7
809
2.20 %
55
6.80 %
691
85.41 %
86
10.63 %
63
7.79 %
0
0.00 %
PAR-RECUR
28
3,456
9.40 %
229
6.63 %
2967
85.85 %
291
8.42 %
257
7.44 %
3
0.09 %
Dorset
PAR-CUL-N
5
1,423
3.87 %
58
4.08 %
1279
89.88 %
100
7.03 %
83
5.83 %
3
0.21 %
PAR-OTHER
2
49
0.13 %
1
2.04 %
47
95.92 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-RECUR
1
432
1.18 %
7
1.62 %
399
92.36 %
28
6.48 %
26
6.02 %
0
0.00 %
Gloucs
PAR-CUL-N
4
43
0.12 %
4
9.30 %
28
65.12 %
11
25.58 %
11
25.58 %
0
0.00 %
PAR-CUL-P
1
195
0.53 %
7
3.59 %
170
87.18 %
17
8.72 %
17
8.72 %
1
0.51 %
PAR-RECUR
7
1,811
4.93 %
38
2.10 %
1674
92.44 %
112
6.18 %
98
5.41 %
1
0.06 %
Heref
PAR-OTHER
2
408
1.11 %
22
5.39 %
372
91.18 %
24
5.88 %
14
3.43 %
0
0.00 %
PAR-PERSIS
6
1,111
3.02 %
61
5.49 %
958
86.23 %
100
9.00 %
89
8.01 %
3
0.27 %
PAR-RECUR
6
352
0.96 %
13
3.69 %
322
91.48 %
23
6.53 %
17
4.83 %
0
0.00 %
Shropshire
PAR-CHR
2
576
1.57 %
6
1.04 %
536
93.06 %
38
6.60 %
34
5.90 %
0
0.00 %
PAR-OTHER
2
212
0.58 %
4
1.89 %
202
95.28 %
7
3.30 %
6
2.83 %
0
0.00 %
PAR-RECUR
21
2,153
5.86 %
44
2.04 %
1953
90.71 %
168
7.80 %
155
7.20 %
1
0.05 %
Somerset excl N
PAR-OTHER
2
83
0.23 %
15
18.07 %
63
75.90 %
6
7.23 %
5
6.02 %
0
0.00 %
PAR-PERSIS
1
217
0.59 %
3
1.38 %
200
92.17 %
17
7.83 %
14
6.45 %
0
0.00 %
PAR-RECUR
6
437
1.19 %
30
6.86 %
392
89.70 %
27
6.18 %
15
3.43 %
0
0.00 %
Staffs
PAR-CUL-N
2
263
0.72 %
6
2.28 %
214
81.37 %
43
16.35 %
43
16.35 %
0
0.00 %
Table 2b. Summary by County for 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
1
425
1.16 %
11
2.59 %
401
94.35 %
17
4.00 %
13
3.06 %
0
0.00 %
PAR-PERSIS
4
739
2.01 %
13
1.76 %
442
59.81 %
63
8.53 %
63
8.53 %
221
29.91 %
PAR-RECUR
7
945
2.57 %
50
5.29 %
790
83.60 %
49
5.19 %
34
3.60 %
71
7.51 %
Wiltshire
PAR-CUL-N
4
312
0.85 %
20
6.41 %
267
85.58 %
27
8.65 %
25
8.01 %
0
0.00 %
PAR-CUL-P
3
712
1.94 %
53
7.44 %
596
83.71 %
52
7.30 %
48
6.74 %
15
2.11 %
PAR-NEW-IN
1
19
0.05 %
1
5.26 %
13
68.42 %
5
26.32 %
5
26.32 %
0
0.00 %
PAR-PERSIS
2
70
0.19 %
7
10.00 %
46
65.71 %
20
28.57 %
17
24.29 %
0
0.00 %
PAR-RECUR
2
404
1.10 %
32
7.92 %
343
84.90 %
30
7.43 %
27
6.68 %
2
0.50 %
England - Edge
Bucks
PAR-NEW-IN
1
13
0.04 %
0
0.00 %
12
92.31 %
1
7.69 %
1
7.69 %
0
0.00 %
Cheshire
PAR-CUL-N
3
1,208
3.29 %
26
2.15 %
1122
92.88 %
72
5.96 %
60
4.97 %
0
0.00 %
PAR-NEW-IN
3
412
1.12 %
15
3.64 %
367
89.08 %
33
8.01 %
30
7.28 %
0
0.00 %
PAR-PERSIS
1
5
0.01 %
0
0.00 %
3
60.00 %
3
60.00 %
2
40.00 %
0
0.00 %
PAR-RECUR
1
98
0.27 %
5
5.10 %
88
89.80 %
6
6.12 %
5
5.10 %
0
0.00 %
Derbyshire
PAR-NEW-IN
4
480
1.31 %
13
2.71 %
419
87.29 %
50
10.42 %
46
9.58 %
2
0.42 %
E Sussex
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
6
0.02 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
4
582
1.58 %
23
3.95 %
527
90.55 %
37
6.36 %
32
5.50 %
0
0.00 %
Northants
PAR-NEW-IN
7
865
2.35 %
15
1.73 %
739
85.43 %
117
13.53 %
111
12.83 %
0
0.00 %
PAR-RECUR
1
114
0.31 %
1
0.88 %
93
81.58 %
11
9.65 %
20
17.54 %
0
0.00 %
Oxon
PAR-NEW-IN
8
923
2.51 %
58
6.28 %
789
85.48 %
86
9.32 %
76
8.23 %
0
0.00 %
PAR-PERSIS
2
160
0.44 %
15
9.38 %
136
85.00 %
9
5.63 %
9
5.63 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
212
0.58 %
2
0.94 %
174
82.08 %
21
9.91 %
18
8.49 %
18
8.49 %
Gr Manchester
PAR-NEW-IN
2
33
0.09 %
0
0.00 %
31
93.94 %
2
6.06 %
2
6.06 %
0
0.00 %
Herts
PAR-NEW-IN
3
250
0.68 %
14
5.60 %
196
78.40 %
41
xx-xxxx-xxxx %
39
15.60 %
1
0.40 %
Lancashire
SER-NSR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northumb
PAR-NEW-IN
1
248
0.67 %
10
4.03 %
208
83.87 %
19
7.66 %
17
6.85 %
13
5.24 %
N Yorks
PAR-NEW-IN
1
151
0.41 %
1
0.66 %
139
92.05 %
11
7.28 %
11
7.28 %
0
0.00 %
W Sussex
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Yorks
PAR-NEW-IN
2
210
0.57 %
5
2.38 %
178
84.76 %
31
14.76 %
27
12.86 %
0
0.00 %
England
218
29477
80.19 %
1235
4.19 %
25729
87.29 %
2445
8.29 %
2151
7.30 %
362
1.23 %
Wales - High TB
Carms
PAR-IR-SEV
6
31
0.08 %
0
0.00 %
16
51.61 %
3
9.68 %
2
6.45 %
13
41.94 %
PAR-RAPID
8
29
0.08 %
5
17.24 %
17
58.62 %
1
3.45 %
1
3.45 %
6
20.69 %
PAR-RECUR
2
22
0.06 %
5
22.73 %
14
63.64 %
5
22.73 %
3
13.64 %
0
0.00 %
SER-FLEXI
6
1,335
3.63 %
74
5.54 %
1197
89.66 %
74
5.54 %
61
4.57 %
3
0.22 %
Ceredigion
PAR-IR-EX
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
19
0.05 %
8
42.11 %
8
42.11 %
1
5.26 %
1
5.26 %
2
10.53 %
PAR-RAPID
5
9
0.02 %
3
33.33 %
3
33.33 %
1
11.11 %
1
11.11 %
2
22.22 %
PAR-RECUR
1
31
0.08 %
10
32.26 %
21
67.74 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
51
0.14 %
2
3.92 %
47
92.16 %
3
5.88 %
2
3.92 %
0
0.00 %
Gwent
PAR-IR-SEV
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
70
0.19 %
0
0.00 %
66
94.29 %
6
8.57 %
3
4.29 %
1
1.43 %
Pembs
PAR-IR-EX
8
38
0.10 %
12
31.58 %
25
65.79 %
2
5.26 %
1
2.63 %
0
0.00 %
PAR-IR-SEV
23
157
0.43 %
40
25.48 %
105
66.88 %
8
5.10 %
7
4.46 %
5
3.18 %
PAR-PERSIS
2
133
0.36 %
4
3.01 %
118
88.72 %
11
8.27 %
11
8.27 %
0
0.00 %
PAR-RAPID
35
88
0.24 %
23
26.14 %
60
68.18 %
4
4.55 %
4
4.55 %
1
1.14 %
PAR-RECUR
8
494
1.34 %
19
3.85 %
448
90.69 %
38
7.69 %
24
4.86 %
3
0.61 %
SER-FLEXI
19
1,525
4.15 %
195
12.79 %
1191
78.10 %
124
8.13 %
107
7.02 %
33
2.16 %
Powys
PAR-IR-EX
2
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
5
18
0.05 %
0
0.00 %
14
77.78 %
5
27.78 %
4
22.22 %
0
0.00 %
PAR-OTHER
5
163
0.44 %
9
5.52 %
137
84.05 %
18
11.04 %
17
10.43 %
0
0.00 %
PAR-PERSIS
1
36
0.10 %
1
2.78 %
31
86.11 %
5
13.89 %
4
11.11 %
0
0.00 %
PAR-POSTM
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
10
23
0.06 %
4
17.39 %
19
82.61 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
8
0.02 %
0
0.00 %
7
87.50 %
2
25.00 %
1
12.50 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.01 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
4
100.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
2
0.01 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
2
100.00 %
PAR-RAPID
2
6
0.02 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
5
0.01 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
3
7
0.02 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
14
0.04 %
3
21.43 %
11
78.57 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
8
446
1.21 %
5
1.12 %
425
95.29 %
17
3.81 %
16
3.59 %
0
0.00 %
PAR-PERSIS
3
191
0.52 %
3
1.57 %
169
88.48 %
18
9.42 %
18
9.42 %
1
0.52 %
PAR-POSTM
8
16
0.04 %
1
6.25 %
14
87.50 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-RAPID
6
12
0.03 %
4
33.33 %
8
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
397
1.08 %
33
8.31 %
347
87.41 %
17
4.28 %
16
4.03 %
1
0.25 %
Powys
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
5
477
1.30 %
19
3.98 %
430
90.15 %
30
6.29 %
26
5.45 %
2
0.42 %
PAR-OTHER
2
214
0.58 %
8
3.74 %
182
85.05 %
28
13.08 %
24
11.21 %
0
0.00 %
PAR-RAPID
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-OTHER
2
8
0.02 %
2
25.00 %
5
62.50 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-RAPID
2
9
0.02 %
7
77.78 %
2
22.22 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
23
0.06 %
7
30.43 %
16
69.57 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-IR-EX
1
4
0.01 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
2
113
0.31 %
3
2.65 %
101
89.38 %
9
7.96 %
9
7.96 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
10
402
1.09 %
24
5.97 %
315
78.36 %
76
18.91 %
63
15.67 %
0
0.00 %
PAR-POSTM
7
21
0.06 %
0
0.00 %
20
95.24 %
1
4.76 %
1
4.76 %
0
0.00 %
NE Wales
PAR-HERD-S
2
40
0.11 %
4
10.00 %
28
70.00 %
8
20.00 %
8
20.00 %
0
0.00 %
PAR-NEW-IN
4
144
0.39 %
0
0.00 %
132
91.67 %
15
10.42 %
10
6.94 %
2
1.39 %
PAR-POSTM
18
35
0.10 %
2
5.71 %
32
91.43 %
2
5.71 %
1
2.86 %
0
0.00 %
PAR-RAPID
2
9
0.02 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
2
384
1.04 %
38
9.90 %
312
81.25 %
40
10.42 %
34
8.85 %
0
0.00 %
Wales
272
7282
19.81 %
583
8.01 %
6135
84.25 %
576
7.91 %
484
6.65 %
81
1.11 %
Scotland - Scotland
Argyll & Bute
SER-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Scotland
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Grand Total
Sum:
491
36761
1818
31866
3021
2635
443
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol February 2022
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
110
14403
100.0%
626
4.3%
12597
87.5%
1321
9.2%
1148
8.0%
32
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
110
14403
100.0%
626
4.3%
12597
87.5%
1321
9.2%
1148
8.0%
32
0.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
103
1501
39.6%
149
9.9%
1228
81.8%
154
10.3%
123
8.2%
1
0.1%
Serial/Extended
16
2294
60.4%
191
8.3%
1973
86.0%
151
6.6%
125
5.4%
5
0.2%
Total
119
3795
100.0%
340
9.0%
3201
84.3%
305
8.0%
248
6.5%
6
0.2%
GB
Parallel
213
15904
87.4%
775
4.9%
13825
86.9%
1475
9.3%
1271
8.0%
33
0.2%
Serial/Extended
16
2294
12.6%
191
8.3%
1973
86.0%
151
6.6%
125
5.4%
5
0.2%
Total
229
18198
100.0%
966
5.3%
15798
86.8%
1626
8.9%
1396
7.7%
38
0.2%
110.00
119.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2022)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
217
29476
100.0%
1235
4.2%
25728
87.3%
2445
8.3%
2151
7.3%
362
1.2%
Serial/Extended
1
1
0.0%
0
0.0 %
1 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
218
29477
100.0%
1235
4.2%
25729
87.3%
2445
8.3%
2151
7.3%
362
1.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
1
2
100.0%
0
0.0 %
2 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
2
100.0%
0
0.0 %
2 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
236
3590
49.3%
241
6.7%
3041
84.7%
318
8.9%
264
7.4%
44
1.2%
Serial/Extended
36
3692
50.7%
342
9.3%
3094
83.8%
258
7.0%
220
6.0%
37
1.0%
Total
272
7282
100.0%
583
8.0%
6135
84.2%
576
7.9%
484
6.6%
81
1.1%
GB
Parallel
453
33066
89.9%
1476
4.5%
28769
87.0%
2763
8.4%
2415
7.3%
406
1.2%
Serial/Extended
38
3695
10.1%
342
9.3%
3097
83.8%
258
7.0%
220
6.0%
37
1.0%
Total
491
36761
100.0%
1818
4.9%
31866
86.7%
3021
8.2%
2635
7.2%
443
1.2%
218.00
272.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
16
2294
12.6%
191
8.3%
1973
86.0%
151
6.6 %
125
5.4 %
5
0.2 %
Parallel - Herd or Group Slaughter
1
6
0.0%
2
33.3%
2
33.3%
2
33.3 %
2
33.3 %
0
Parallel - IRs in Exit Strategy for persistent her
9
33
0.2%
9
27.3%
22
66.7%
2
6.1 %
2
6.1 %
0
Parallel New Infection
37
3108
17.1%
106
3.4%
2644
85.1%
378
12.2 %
337
10.8 %
21
0.7 %
Parallel - new infection cull area
12
2353
12.9%
112
4.8%
2038
86.6%
228
9.7 %
200
8.5 %
3
0.1 %
Parallel - Other
8
548
3.0%
40
7.3%
487
88.9%
32
5.8 %
21
3.8 %
0
Parallel Persistent Infection
15
xx-xxxx-xxxx
9.0%
91
5.5%
1400
85.1%
181
11.0 %
152
9.2 %
3
0.2 %
Parallel - persistent infection cull area
3
28
0.2%
2
7.1%
18
64.3%
8
28.6 %
8
28.6 %
0
Parallel – post-mvt test coupled with IDEXX an
10
17
0.1%
2
11.8%
13
76.5%
3
17.6 %
2
11.8 %
0
Parallel - Rapid Testing Two-times IRs
42
100
0.5%
34
34.0%
63
63.0%
3
3.0 %
3
3.0 %
0
Parallel - Recurrent breakdowns
57
7372
40.5%
330
4.5%
6533
88.6%
592
8.0 %
503
6.8 %
6
0.1 %
Parallel - severe IRs - in persistent herds
17
117
0.6%
41
35.0%
69
59.0%
8
6.8 %
7
6.0 %
0
PAR-CHR
2
576
3.2%
6
1.0%
536
93.1%
38
6.6 %
34
5.9 %
0
Total
229
18198
100.0%
966
5.3%
15798
86.8%
1626
8.9 %
1396
7.7 %
38
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (February 2022)
England - HRA
Parallel - new infection cull area
11
2325
12.8%
111
4.8%
2018
86.8%
221
9.5 %
193
8.3 %
3
0.1 %
Parallel - Other
6
540
3.0%
38
7.0%
482
89.3%
31
5.7 %
20
3.7 %
0
Parallel Persistent Infection
14
xx-xxxx-xxxx
9.0%
91
5.5%
1397
85.1%
178
10.8 %
150
9.1 %
3
0.2 %
Parallel - persistent infection cull area
3
28
0.2%
2
7.1%
18
64.3%
8
28.6 %
8
28.6 %
0
Parallel - Recurrent breakdowns
51
6979
38.4%
309
4.4%
6189
88.7%
553
7.9 %
476
6.8 %
5
0.1 %
PAR-CHR
2
576
3.2%
6
1.0%
536
93.1%
38
6.6 %
34
5.9 %
0
England - HRA
87
12089
66.4%
557
4.6%
10640
88.0%
1029
8.5 %
881
7.3 %
11
0.1 %
England - Edge
Parallel New Infection
14
1481
8.1%
44
3.0%
1270
85.8%
180
12.2 %
165
11.1 %
2
0.1 %
Parallel - new infection cull area
1
28
0.2%
1
3.6%
20
71.4%
7
25.0 %
7
25.0 %
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
3
60.0%
3
60.0 %
2
40.0 %
0
Parallel - Recurrent breakdowns
1
98
0.5%
5
5.1%
88
89.8%
6
6.1 %
5
5.1 %
0
England - Edge
17
1612
8.9%
50
3.1%
1381
85.7%
196
12.2 %
179
11.1 %
2
0.1 %
England - LRA
Parallel New Infection
6
702
3.9%
19
2.7%
576
82.1%
96
13.7 %
88
12.5 %
19
2.7 %
England - LRA
6
702
3.9%
19
2.7%
576
82.1%
96
13.7 %
88
12.5 %
19
2.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
16
2294
12.6%
191
8.3%
1973
86.0%
151
6.6 %
125
5.4 %
5
0.2 %
Parallel - IRs in Exit Strategy for persistent her
6
25
0.1%
9
36.0%
15
60.0%
1
4.0 %
1
4.0 %
0
Parallel – post-mvt test coupled with IDEXX an
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
36
82
0.5%
25
30.5%
54
65.9%
3
3.7 %
3
3.7 %
0
Parallel - Recurrent breakdowns
3
266
1.5%
8
3.0%
238
89.5%
30
11.3 %
19
7.1 %
1
0.4 %
Parallel - severe IRs - in persistent herds
16
116
0.6%
40
34.5%
69
59.5%
8
6.9 %
7
6.0 %
0
Wales - High TB
78
2785
15.3%
273
9.8%
2351
84.4%
193
6.9 %
155
5.6 %
6
0.2 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
3
8
0.0%
0
0.0 %
7
87.5%
1
12.5 %
1
12.5 %
0
Parallel New Infection
9
571
3.1%
20
3.5%
521
91.2%
35
6.1 %
30
5.3 %
0
Parallel - Other
2
8
0.0%
2
25.0%
5
62.5%
1
12.5 %
1
12.5 %
0
Parallel – post-mvt test coupled with IDEXX an
3
5
0.0%
1
20.0%
3
60.0%
1
20.0 %
1
20.0 %
0
Parallel - Rapid Testing Two-times IRs
5
17
0.1%
8
47.1%
9
52.9%
0
0
0
Parallel - Recurrent breakdowns
2
29
0.2%
8
27.6%
18
62.1%
3
10.3 %
3
10.3 %
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales - Intermediate TB
25
639
3.5%
40
6.3%
563
88.1%
41
6.4 %
36
5.6 %
0
Wales - Low TB
Parallel - Herd or Group Slaughter
1
6
0.0%
2
33.3%
2
33.3%
2
33.3 %
2
33.3 %
0
Parallel New Infection
8
354
1.9%
23
6.5%
277
78.2%
67
18.9 %
54
15.3 %
0
Parallel – post-mvt test coupled with IDEXX an
6
10
0.1%
1
10.0%
8
80.0%
2
20.0 %
1
10.0 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales - Low TB
16
371
2.0%
27
7.3%
287
77.4%
71
19.1 %
57
15.4 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
36
3692
10.0%
342
9.3%
3094
83.8%
258
7.0 %
220
6.0 %
37
1.0 %
Parallel - Herd or Group Slaughter
2
40
0.1%
4
10.0%
28
70.0%
8
20.0 %
8
20.0 %
0
Parallel - IRs in Exit Strategy for persistent herds
17
56
0.2%
13
23.2%
41
73.2%
3
5.4 %
2
3.6 %
0
Parallel New Infection
68
5876
16.0%
206
3.5%
5102
86.8%
592
10.1 %
530
9.0 %
38
0.6 %
Parallel - new infection cull area
27
4742
12.9%
199
4.2%
4209
88.8%
376
7.9 %
329
6.9 %
5
0.1 %
Parallel - Other
18
1139
3.1%
61
5.4%
1008
88.5%
85
7.5 %
68
6.0 %
2
0.2 %
Parallel Persistent Infection
37
5154
14.0%
235
4.6%
4285
83.1%
469
9.1 %
409
7.9 %
225
4.4 %
Parallel - persistent infection cull area
9
1770
4.8%
81
4.6%
1566
88.5%
122
6.9 %
105
5.9 %
18
1.0 %
Parallel – post-mvt test coupled with IDEXX anti
34
74
0.2%
3
4.1%
68
91.9%
4
5.4 %
3
4.1 %
0
Parallel - Rapid Testing Two-times IRs
74
198
0.5%
51
25.8%
131
66.2%
7
3.5 %
7
3.5 %
9
4.5 %
Parallel - Recurrent breakdowns
118
13196
35.9%
566
4.3%
11639
88.2%
1042
7.9 %
906
6.9 %
85
0.6 %
Parallel - severe IRs - in persistent herds
47
245
0.7%
51
20.8%
156
63.7%
17
6.9 %
14
5.7 %
24
9.8 %
PAR-CHR
2
576
1.6%
6
1.0%
536
93.1%
38
6.6 %
34
5.9 %
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Total
491
36761
100.0%
1818
4.9%
31866
86.7%
3021
8.2 %
2635
7.2 %
443
1.2 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2022)
England - HRA
Parallel New Infection
1
19
0.1%
1
5.3%
13
68.4%
5
26.3 %
5
26.3 %
0
Parallel - new infection cull area
24
3534
9.6%
173
4.9%
3087
87.4%
304
8.6 %
269
7.6 %
5
0.1 %
Parallel - Other
8
752
2.0%
42
5.6%
684
91.0%
38
5.1 %
26
3.5 %
0
Parallel Persistent Infection
28
4629
12.6%
212
4.6%
3828
82.7%
423
9.1 %
365
7.9 %
224
4.8 %
Parallel - persistent infection cull area
9
1770
4.8%
81
4.6%
1566
88.5%
122
6.9 %
105
5.9 %
18
1.0 %
Parallel - Recurrent breakdowns
97
12223
33.2%
516
4.2%
10785
88.2%
965
7.9 %
841
6.9 %
81
0.7 %
PAR-CHR
2
576
1.6%
6
1.0%
536
93.1%
38
6.6 %
34
5.9 %
0
England - Edge
Parallel New Infection
29
3283
8.9%
125
3.8%
2860
87.1%
324
9.9 %
296
9.0 %
2
0.1 %
Parallel - new infection cull area
3
1208
3.3%
26
2.2%
1122
92.9%
72
6.0 %
60
5.0 %
0
Parallel Persistent Infection
3
165
0.4%
15
9.1%
139
84.2%
12
7.3 %
11
6.7 %
0
Parallel - Recurrent breakdowns
2
212
0.6%
6
2.8%
181
85.4%
17
8.0 %
25
11.8 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
England - LRA
Parallel New Infection
11
1105
3.0%
32
2.9%
927
83.9%
125
11.3 %
114
10.3 %
32
2.9 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
England
218
29477
80.2%
1235
4.2%
25729
87.3%
2445
8.3 %
2151
7.3 %
362
1.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
27
2911
7.9%
271
9.3%
2435
83.6%
201
6.9 %
170
5.8 %
36
1.2 %
Parallel - IRs in Exit Strategy for persistent herd
12
44
0.1%
12
27.3%
31
70.5%
2
4.5 %
1
2.3 %
0
Parallel - Other
5
163
0.4%
9
5.5%
137
84.0%
18
11.0 %
17
10.4 %
0
Parallel Persistent Infection
3
169
0.5%
5
3.0%
149
88.2%
16
9.5 %
15
8.9 %
0
Parallel – post-mvt test coupled with IDEXX ant
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
60
154
0.4%
35
22.7%
104
67.5%
6
3.9 %
6
3.9 %
9
5.8 %
Parallel - Recurrent breakdowns
16
625
1.7%
34
5.4%
556
89.0%
51
8.2 %
31
5.0 %
4
0.6 %
Parallel - severe IRs - in persistent herds
42
227
0.6%
48
21.1%
145
63.9%
17
7.5 %
14
6.2 %
20
8.8 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
397
1.1%
33
8.3%
347
87.4%
17
4.3 %
16
4.0 %
1
0.3 %
Parallel - IRs in Exit Strategy for persistent herd
5
12
0.0%
1
8.3%
10
83.3%
1
8.3 %
1
8.3 %
0
Parallel New Infection
13
923
2.5%
24
2.6%
855
92.6%
47
5.1 %
42
4.6 %
2
0.2 %
Parallel - Other
5
224
0.6%
10
4.5%
187
83.5%
29
12.9 %
25
11.2 %
2
0.9 %
Parallel Persistent Infection
3
191
0.5%
3
1.6%
169
88.5%
18
9.4 %
18
9.4 %
1
0.5 %
Parallel – post-mvt test coupled with IDEXX ant
8
16
0.0%
1
6.3%
14
87.5%
1
6.3 %
1
6.3 %
0
Parallel - Rapid Testing Two-times IRs
12
35
0.1%
15
42.9%
20
57.1%
0
0
0
Parallel - Recurrent breakdowns
3
136
0.4%
10
7.4%
117
86.0%
9
6.6 %
9
6.6 %
0
Parallel - severe IRs - in persistent herds
5
18
0.0%
3
16.7%
11
61.1%
0
0
4
22.2 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
384
1.0%
38
9.9%
312
81.3%
40
10.4 %
34
8.9 %
0
Parallel - Herd or Group Slaughter
2
40
0.1%
4
10.0%
28
70.0%
8
20.0 %
8
20.0 %
0
Parallel New Infection
14
546
1.5%
24
4.4%
447
81.9%
91
16.7 %
73
13.4 %
2
0.4 %
Parallel – post-mvt test coupled with IDEXX ant
25
56
0.2%
2
3.6%
52
92.9%
3
5.4 %
2
3.6 %
0
Parallel - Rapid Testing Two-times IRs
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Wales
272
7282
19.8%
583
8.0%
6135
84.2%
576
7.9 %
484
6.6 %
81
1.1 %
Scotland - Scotland
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Scotland
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2021 March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
4
0
9
0
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
4
0
4
0
1083
0
92
0
6
July
298
24841
164
1077
30
0
0
0
0
61
4
0
4
0
936
0
40
0
2
August
312
17920
167
912
3
26
0
0
0
0
35
2
0
7
0
755
0
81
1
2
September
274
15805
130
890
13
0
0
0
0
95
1
0
7
0
742
0
31
0
0
October
276
19790
140
814
15
0
0
0
0
44
3
2
17
0
696
0
36
1
0
November
329
25737
195
1349
55
0
0
0
0
92
0
1
22
1
1117
0
61
0
0
December
238
13117
138
759
14
0
0
0
0
42
0
5
1
2
656
8
25
0
5
2022 January
262
18563
129
852
27
0
0
0
0
32
0
7
11
0
739
35
0
0
0
February
229
18198
132
966
81
394
0
0
0
0
51
0
1
0
0
332
22
0
0
0
Totals (last
12 months)
3716
273433
2084
13935
88
717
0
0
0
0
869
28
16
103
3
11271
66
665
4
16
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1832
211705
1187
10014
589
0
0
0
0
769
11
11
53
3
8424
1
67
0
0
Scotland
18
1604
12
115
0
0
0
0
0
47
0
0
0
0
53
0
10
0
5
Wales
1866
60124
885
3806
128
0
0
0
0
53
17
5
50
0
2794
65
588
4
11
Totals
3716
273433
2084
13935
717
0
0
0
0
869
28
16
103
3
11271
66
665
4
16
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2020
2021
2022
IFNg Positive herds
IFNg Negative herds
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2020
2021
2022
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2020
2021
2022
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
W Yorks
Wiltshire
W Glamorgan
Staffs
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
N Yorks
Northants
NE Wales
Leics & Rut
Herts
Heref
Gwynedd
Gloucs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during February 2022 (% submission per
County)
County
%Resampled %Retested
Avon
9.09 %
9.74 %
Carms
4.58 %
5.61 %
Ceredigion
4.00 %
4.00 %
Cheshire
13.01 %
14.38 %
Corn & Scilly
9.91 %
11.66 %
Cumbria
8.49 %
9.91 %
Derbyshire
10.33 %
11.33 %
Devon
7.88 %
9.63 %
Dorset
5.78 %
6.78 %
Gloucs
5.13 %
5.91 %
Gwynedd
15.56 %
19.02 %
Heref
7.03 %
8.32 %
Herts
15.60 %
xx-xxxx-xxxx %
Leics & Rut
10.60 %
13.25 %
NE Wales
4.73 %
5.62 %
Northants
12.01 %
12.52 %
N Yorks
7.28 %
7.28 %
Oxon
9.95 %
11.11 %
Pembs
6.49 %
8.23 %
Powys
6.64 %
7.97 %
S Glamorgan
2.94 %
2.94 %
Shropshire
5.92 %
6.76 %
Somerset excl
4.66 %
5.38 %
Staffs
10.94 %
11.82 %
W Glamorgan
50.00 %
50.00 %
Wiltshire
7.72 %
8.36 %
W Yorks
22.47 %
25.84 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
14132
626
4.4%
14403
626
4.3%
Wales
3742
340
9.1%
3795
340
9.0%
GB
17874
966
5.4%
18198
966
5.3%
Table 6a (i): Number of animals and submissions tested in February 2022
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
28296
1235
4.4%
29477
1235
4.2%
Wales
6969
583
8.4%
7282
583
8.0%
Scotland
2
0
0.0 %
2
0.0 %
GB
35267
1818
5.2%
36761
1818
4.9%
Table 6b (i): Number of animals and submissions tested in 2022 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
21756
1205
5.5%
22216
1205
5.4%
Wales
6008
398
6.6%
6142
398
6.5%
Scotland
369
34
9.2%
401
34
8.5%
GB
28133
1637
5.8%
28759
1637
5.7%
Table 6a (ii): Number of animals and submissions tested in February 2021
Table 6b (ii): Number of animals and submissions tested in 2021 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
43840
2559
5.8%
45341
2559
5.6%
Wales
10526
696
6.6%
10937
696
6.4%
Scotland
389
35
9.0%
421
35
8.3%
GB
54753
3290
6.0%
56699
3290
5.8%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Somerset excl North
PRV-SP
1
4
0
0
Total
2
6
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples February 2022
Table 7b Number of private samples 2022
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Hampshire
PRV-SE
1
7
2
28.57 %
Somerset excl North
PRV-SP
1
4
0
0
Wiltshire
PRV-SE
1
1
0
0
Total
4
14
2
14.29 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Not Known
BOV-POSTM
1
1
1
100.0%
Not Known
1
1
1
100.0%
Wales
Carmarthenshire
BOV-IR-C
2
12
2
16.7%
Ceredigion
BOV-IR-C
3
18
5
27.8%
North East Wales
BOV-IR-C
1
1
1
100.0%
BOV-IR-EX
1
3
1
33.3%
BOV-POSTM
6
10
3
30.0%
Pembrokeshire
BOV-IR-C
8
102
23
22.5%
BOV-IR-EX
2
3
1
33.3%
BOV-OTHER
2
160
4
2.5%
Powys
BOV-IR-C
2
15
1
6.7%
Wales
27
324
41
12.7%
All GB
28
325
42
12.9 %
Table 8a Number of Cattle Antibody Tests February 2022
Table 8b Number of Cattle Antibody Tests 2022
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Not Known
BOV-POSTM
1
1
1
100.0%
Not Known
1
1
1
100.0%
Wales
Carmarthenshire
BOV-IR-C
3
31
2
6.5%
Ceredigion
BOV-IR-C
5
66
8
12.1%
North East Wales
BOV-IR-C
7
90
14
15.6%
BOV-IR-EX
1
3
1
33.3%
BOV-OTHER
1
17
0
0
BOV-P
1
420
43
10.2%
BOV-POSTM
14
33
9
27.3%
Pembrokeshire
BOV-IR-C
20
191
41
21.5%
BOV-IR-EX
4
14
3
21.4%
BOV-OTHER
3
176
6
3.4%
BOV-P
1
115
15
13.0%
Powys
BOV-IR-C
4
28
4
14.3%
South Wales
BOV-IR-C
1
4
0
0
Wales
65
1,188
146
12.3%
All GB
66
1,189
147
12.4 %