Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - February 2023
Source: environment.data.gov.uk (2024-07-03)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2023. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 198
Report Period 1st - 28th February 2023
Issued 13 March 2023

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
February
2023
%
Total 2023
%
Submissions
265
546
Samples
Total (%)
17538
100.00 %
41234
100.00 %
England
11362
64.79 %
26916
65.28 %
Wales
5516
31.45 %
12730
30.87 %
Scotland
660
3.76 %
1588
3.85 %
Parallel Tests
PAR-CHR
0
0.00 %
PAR-CUL-N
809
4.61 %
811
1.97 %
PAR-CUL-P
0
0.00 %
PAR-HERD-S
0
0.00 %
PAR-IR-EX
50
0.29 %
102
0.25 %
PAR-IR-SEV
227
1.29 %
436
1.06 %
PAR-NEW-IN
5990
34.15 %
15737
38.17 %
PAR-OTHER
588
3.35 %
872
2.11 %
PAR-PERSIS
1748
9.97 %
2371
5.75 %
PAR-POSTM
15
0.09 %
34
0.08 %
PAR-RAPID
66
0.38 %
156
0.38 %
PAR-RECUR
6957
39.67 %
19025
46.14 %
Total(% of all samples)
xx-xxxx-xxxx
93.80 %
39544
95.90 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
1088
6.20 %
xx-xxxx-xxxx
4.10 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
1088
6.20 %
xx-xxxx-xxxx
4.10 %
Total
17538
100.00 %
41234
100.00 %
Retests
Total (% of all samples)
xx-xxxx-xxxx
9.36 %
3483
8.45 %
England
1094
6.24 %
2118
5.14 %
Wales
480
2.74 %
1213
2.94 %
Scotland
68
0.39 %
152
0.37 %
Resamples
Total (% of all samples)
1398
7.97 %
2846
6.90 %
England
926
5.28 %
1823
4.42 %
Wales
406
2.31 %
878
2.13 %
Scotland
66
0.38 %
145
0.35 %
Rejects
Total (% of all samples)
92
0.52 %
1453
3.52 %
England
7
0.04 %
1094
2.65 %
Wales
40
0.23 %
296
0.72 %
Scotland
45
0.26 %
63
0.15 %
Table 1. Monthly Totals for February 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
809
4.6 %
PAR-IR-EX
50
0.3 %
PAR-IR-SEV
227
1.3 %
PAR-NEW-IN
5990
34.2 %
PAR-OTHER
588
3.4 %
PAR-PERSIS
1748
10.0 %
PAR-POSTM
15
0.1 %
PAR-RAPID
66
0.4 %
PAR-RECUR
6957
39.7 %
SER-FLEXI
1088
6.2 %
Total
17538
100.00 %
Figure 1. Number of samples submitted in each category during February 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
Wigtown
West lothian
Ayrshire
W Glamorgan
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during February 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
1000
2000
3000
4000
5000
6000
Wigtown
West lothian
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NW Wales
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
3
74
0.42 %
6
8.11 %
68
91.89 %
1
1.35 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
2
809
4.61 %
60
7.42 %
692
85.54 %
67
8.28 %
57
7.05 %
0
0.00 %
PAR-RECUR
6
1,761
10.04 %
63
3.58 %
1539
87.39 %
171
9.71 %
158
8.97 %
1
0.06 %
Devon
PAR-RECUR
8
1,554
8.86 %
165
10.62 %
1285
82.69 %
135
8.69 %
104
6.69 %
0
0.00 %
Gloucs
PAR-RECUR
4
96
0.55 %
4
4.17 %
79
82.29 %
16
16.67 %
13
13.54 %
0
0.00 %
Heref
PAR-RECUR
6
545
3.11 %
21
3.85 %
457
83.85 %
77
14.13 %
67
12.29 %
0
0.00 %
Shropshire
PAR-RECUR
13
1,022
5.83 %
68
6.65 %
856
83.76 %
129
12.62 %
97
9.49 %
1
0.10 %
Somerset excl N
PAR-RECUR
3
12
0.07 %
1
8.33 %
9
75.00 %
2
16.67 %
2
16.67 %
0
0.00 %
Staffs
PAR-RECUR
4
408
2.33 %
11
2.70 %
320
78.43 %
79
19.36 %
77
18.87 %
0
0.00 %
Wiltshire
PAR-RECUR
3
649
3.70 %
37
5.70 %
594
91.53 %
25
3.85 %
16
2.47 %
2
0.31 %
England - Edge
Berks
PAR-NEW-IN
1
193
1.10 %
2
1.04 %
178
92.23 %
13
6.74 %
13
6.74 %
0
0.00 %
PAR-RECUR
1
132
0.75 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-NEW-IN
5
208
1.19 %
10
4.81 %
185
88.94 %
14
6.73 %
13
6.25 %
0
0.00 %
Cheshire
PAR-OTHER
2
511
2.91 %
16
3.13 %
488
95.50 %
7
1.37 %
7
1.37 %
0
0.00 %
PAR-PERSIS
8
1,187
6.77 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
376
2.14 %
14
3.72 %
328
87.23 %
38
10.11 %
34
9.04 %
0
0.00 %
PAR-RECUR
1
32
0.18 %
1
3.13 %
24
75.00 %
7
21.88 %
7
21.88 %
0
0.00 %
E Sussex
PAR-NEW-IN
3
239
1.36 %
9
3.77 %
204
85.36 %
32
13.39 %
26
10.88 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
218
1.24 %
9
4.13 %
197
90.37 %
17
7.80 %
11
5.05 %
1
0.46 %
Leics & Rut
PAR-NEW-IN
3
481
2.74 %
11
2.29 %
450
93.56 %
29
6.03 %
20
4.16 %
0
0.00 %
Northants
PAR-NEW-IN
1
14
0.08 %
1
7.14 %
8
57.14 %
6
42.86 %
5
35.71 %
0
0.00 %
Notts
PAR-NEW-IN
2
62
0.35 %
4
6.45 %
54
87.10 %
6
9.68 %
4
6.45 %
0
0.00 %
Oxon
PAR-NEW-IN
1
177
1.01 %
4
2.26 %
160
90.40 %
17
9.60 %
13
7.34 %
0
0.00 %
Warks
PAR-RECUR
1
50
0.29 %
1
2.00 %
43
86.00 %
6
12.00 %
6
12.00 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
158
0.90 %
2
1.27 %
126
79.75 %
38
24.05 %
30
18.99 %
0
0.00 %
Essex
PAR-NEW-IN
2
176
1.00 %
1
0.57 %
173
98.30 %
4
2.27 %
2
1.14 %
0
0.00 %
Table 2a. Summary by County for February 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gr Manchester
PAR-PERSIS
1
41
0.23 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
1
40
0.23 %
0
0.00 %
39
97.50 %
1
2.50 %
1
2.50 %
0
0.00 %
Surrey
PAR-NEW-IN
2
68
0.39 %
0
0.00 %
54
79.41 %
11
16.18 %
13
19.12 %
1
1.47 %
W Yorks
PAR-NEW-IN
1
69
0.39 %
0
0.00 %
65
94.20 %
5
7.25 %
4
5.80 %
0
0.00 %
England
93
11362
64.79 %
555
4.88 %
9874
86.90 %
1094
9.63 %
926
8.15 %
7
0.06 %
Wales - High TB
Carms
PAR-IR-EX
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
57
0.33 %
21
36.84 %
34
59.65 %
2
3.51 %
2
3.51 %
0
0.00 %
PAR-PERSIS
1
9
0.05 %
0
0.00 %
5
55.56 %
3
33.33 %
3
33.33 %
1
11.11 %
PAR-RAPID
4
8
0.05 %
4
50.00 %
3
37.50 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-RECUR
1
5
0.03 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
SER-FLEXI
4
338
1.93 %
26
7.69 %
295
87.28 %
18
5.33 %
15
4.44 %
2
0.59 %
Ceredigion
PAR-IR-SEV
3
5
0.03 %
2
40.00 %
2
40.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
391
2.23 %
68
17.39 %
308
78.77 %
24
6.14 %
15
3.84 %
0
0.00 %
Gwent
PAR-IR-EX
1
8
0.05 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
13
0.07 %
9
69.23 %
3
23.08 %
1
7.69 %
1
7.69 %
0
0.00 %
PAR-RAPID
2
9
0.05 %
3
33.33 %
6
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
4
0.02 %
1
25.00 %
2
50.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Pembs
PAR-IR-EX
8
29
0.17 %
13
44.83 %
14
48.28 %
1
3.45 %
1
3.45 %
1
3.45 %
PAR-IR-SEV
8
45
0.26 %
13
28.89 %
30
66.67 %
2
4.44 %
2
4.44 %
0
0.00 %
PAR-OTHER
1
14
0.08 %
5
35.71 %
8
57.14 %
1
7.14 %
1
7.14 %
0
0.00 %
PAR-PERSIS
3
181
1.03 %
16
8.84 %
145
80.11 %
26
14.36 %
20
11.05 %
0
0.00 %
PAR-RAPID
7
17
0.10 %
8
47.06 %
7
41.18 %
1
5.88 %
1
5.88 %
1
5.88 %
PAR-RECUR
3
451
2.57 %
31
6.87 %
374
82.93 %
32
7.10 %
25
5.54 %
21
4.66 %
SER-FLEXI
2
298
1.70 %
17
5.70 %
272
91.28 %
15
5.03 %
8
2.68 %
1
0.34 %
Powys
PAR-IR-EX
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-IR-SEV
10
80
0.46 %
11
13.75 %
60
75.00 %
11
13.75 %
8
10.00 %
1
1.25 %
PAR-PERSIS
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
8
15
0.09 %
2
13.33 %
13
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
16
0.09 %
3
18.75 %
10
62.50 %
3
18.75 %
3
18.75 %
0
0.00 %
S Wales
PAR-IR-SEV
1
9
0.05 %
6
66.67 %
3
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
63
0.36 %
5
7.94 %
46
73.02 %
13
20.63 %
12
19.05 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.02 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
4
28
0.16 %
0
0.00 %
27
96.43 %
2
7.14 %
1
3.57 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
318
1.81 %
41
12.89 %
254
79.87 %
27
8.49 %
23
7.23 %
0
0.00 %
NE Wales
PAR-IR-EX
3
9
0.05 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
13
0.07 %
4
30.77 %
9
69.23 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
875
4.99 %
25
2.86 %
766
87.54 %
83
9.49 %
77
8.80 %
7
0.80 %
PAR-PERSIS
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
PAR-POSTM
3
9
0.05 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-RAPID
6
8
0.05 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
61
0.35 %
6
9.84 %
53
86.89 %
2
3.28 %
2
3.28 %
0
0.00 %
Powys
PAR-NEW-IN
1
10
0.06 %
1
10.00 %
8
80.00 %
1
10.00 %
1
10.00 %
0
0.00 %
PAR-RECUR
1
105
0.60 %
0
0.00 %
99
94.29 %
6
5.71 %
6
5.71 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
13
765
4.36 %
39
5.10 %
651
85.10 %
81
10.59 %
75
9.80 %
0
0.00 %
PAR-PERSIS
1
7
0.04 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
3
3
0.02 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
13
0.07 %
0
0.00 %
12
92.31 %
1
7.69 %
1
7.69 %
0
0.00 %
NE Wales
PAR-NEW-IN
18
1,201
6.85 %
20
1.67 %
1082
90.09 %
115
9.58 %
94
7.83 %
5
0.42 %
PAR-POSTM
2
3
0.02 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
164
5516
31.45 %
418
7.58 %
4652
84.34 %
480
8.70 %
406
7.36 %
40
0.73 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
279
1.59 %
38
13.62 %
209
74.91 %
32
11.47 %
31
11.11 %
1
0.36 %
West lothian
PAR-NEW-IN
4
354
2.02 %
24
6.78 %
258
72.88 %
29
8.19 %
28
7.91 %
44
12.43 %
Wigtown
PAR-NEW-IN
1
27
0.15 %
1
3.70 %
19
70.37 %
7
25.93 %
7
25.93 %
0
0.00 %
Scotland
8
660
3.76 %
63
9.55 %
486
73.64 %
68
10.30 %
66
10.00 %
45
6.82 %
Grand Total
Sum:
265
17538
1036
15012
xx-xxxx-xxxx
1398
92
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
7
298
0.72 %
12
4.03 %
268
89.93 %
19
6.38 %
17
5.70 %
1
0.34 %
Corn & Scilly
PAR-CUL-N
2
809
1.96 %
60
7.42 %
692
85.54 %
67
8.28 %
57
7.05 %
0
0.00 %
PAR-RECUR
12
4,696
11.39 %
208
4.43 %
4132
87.99 %
388
8.26 %
355
7.56 %
1
0.02 %
Devon
PAR-CUL-N
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RECUR
12
2,551
6.19 %
183
7.17 %
1959
76.79 %
179
7.02 %
143
5.61 %
266
10.43 %
Dorset
PAR-RECUR
2
717
1.74 %
12
1.67 %
321
44.77 %
22
3.07 %
22
3.07 %
362
50.49 %
Gloucs
PAR-RECUR
5
534
1.30 %
25
4.68 %
475
88.95 %
40
7.49 %
34
6.37 %
0
0.00 %
Heref
PAR-RECUR
12
2,647
6.42 %
160
6.04 %
2293
86.63 %
222
8.39 %
194
7.33 %
0
0.00 %
Shropshire
PAR-RECUR
21
1,604
3.89 %
84
5.24 %
1394
86.91 %
161
10.04 %
124
7.73 %
2
0.12 %
Somerset excl N
PAR-RECUR
5
365
0.89 %
12
3.29 %
338
92.60 %
16
4.38 %
14
3.84 %
1
0.27 %
Staffs
PAR-RECUR
11
1,377
3.34 %
33
2.40 %
1155
83.88 %
171
12.42 %
160
11.62 %
29
2.11 %
Wiltshire
PAR-RECUR
5
816
1.98 %
40
4.90 %
752
92.16 %
36
4.41 %
22
2.70 %
2
0.25 %
England - Edge
Berks
PAR-NEW-IN
4
784
1.90 %
10
1.28 %
726
92.60 %
50
6.38 %
48
6.12 %
0
0.00 %
PAR-RECUR
1
132
0.32 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-NEW-IN
8
703
1.70 %
26
3.70 %
642
91.32 %
37
5.26 %
35
4.98 %
0
0.00 %
Cheshire
PAR-OTHER
2
511
1.24 %
16
3.13 %
488
95.50 %
7
1.37 %
7
1.37 %
0
0.00 %
PAR-PERSIS
8
1,187
2.88 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
PAR-RECUR
2
215
0.52 %
2
0.93 %
201
93.49 %
15
6.98 %
12
5.58 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
376
0.91 %
14
3.72 %
328
87.23 %
38
10.11 %
34
9.04 %
0
0.00 %
PAR-RECUR
5
1,010
2.45 %
32
3.17 %
905
89.60 %
79
7.82 %
73
7.23 %
0
0.00 %
E Sussex
PAR-NEW-IN
3
239
0.58 %
9
3.77 %
204
85.36 %
32
13.39 %
26
10.88 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
218
0.53 %
9
4.13 %
197
90.37 %
17
7.80 %
11
5.05 %
1
0.46 %
Leics & Rut
PAR-NEW-IN
5
650
1.58 %
20
3.08 %
597
91.85 %
45
6.92 %
33
5.08 %
0
0.00 %
Northants
PAR-NEW-IN
6
786
1.91 %
17
2.16 %
598
76.08 %
63
8.02 %
53
6.74 %
118
15.01 %
Notts
PAR-NEW-IN
5
292
0.71 %
9
3.08 %
265
90.75 %
34
11.64 %
16
5.48 %
2
0.68 %
Oxon
PAR-NEW-IN
1
177
0.43 %
4
2.26 %
160
90.40 %
17
9.60 %
13
7.34 %
0
0.00 %
Table 2b. Summary by County for 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
59
0.14 %
11
18.64 %
46
77.97 %
4
6.78 %
2
3.39 %
0
0.00 %
Warks
PAR-OTHER
1
19
0.05 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
19
100.00 %
PAR-RECUR
8
140
0.34 %
5
3.57 %
105
75.00 %
18
12.86 %
16
11.43 %
14
10.00 %
England - LRA
Cumbria
PAR-NEW-IN
5
1,517
3.68 %
51
3.36 %
1340
88.33 %
146
9.62 %
126
8.31 %
0
0.00 %
Essex
PAR-NEW-IN
4
353
0.86 %
1
0.28 %
173
49.01 %
4
1.13 %
2
0.57 %
177
50.14 %
Gr Manchester
PAR-PERSIS
1
41
0.10 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
75
0.18 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Lincs
PAR-NEW-IN
3
400
0.97 %
7
1.75 %
381
95.25 %
12
3.00 %
12
3.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
1
40
0.10 %
0
0.00 %
39
97.50 %
1
2.50 %
1
2.50 %
0
0.00 %
Surrey
PAR-NEW-IN
4
507
1.23 %
22
4.34 %
358
70.61 %
29
5.72 %
29
5.72 %
98
19.33 %
W Yorks
PAR-NEW-IN
1
69
0.17 %
0
0.00 %
65
94.20 %
5
7.25 %
4
5.80 %
0
0.00 %
England
180
26916
65.28 %
1130
4.20 %
22869
84.96 %
2118
7.87 %
1823
6.77 %
1094
4.06 %
Wales - High TB
Carms
PAR-IR-EX
4
11
0.03 %
7
63.64 %
4
36.36 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
8
84
0.20 %
38
45.24 %
40
47.62 %
6
7.14 %
6
7.14 %
0
0.00 %
PAR-PERSIS
4
124
0.30 %
0
0.00 %
109
87.90 %
13
10.48 %
11
8.87 %
4
3.23 %
PAR-RAPID
13
34
0.08 %
13
38.24 %
18
52.94 %
3
8.82 %
2
5.88 %
1
2.94 %
PAR-RECUR
2
127
0.31 %
18
14.17 %
101
79.53 %
8
6.30 %
8
6.30 %
0
0.00 %
SER-FLEXI
4
338
0.82 %
26
7.69 %
295
87.28 %
18
5.33 %
15
4.44 %
2
0.59 %
Ceredigion
PAR-IR-EX
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
5
9
0.02 %
6
66.67 %
2
22.22 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-RAPID
3
4
0.01 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
6
441
1.07 %
82
18.59 %
340
77.10 %
31
7.03 %
19
4.31 %
0
0.00 %
Gwent
PAR-IR-EX
1
8
0.02 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
13
0.03 %
9
69.23 %
3
23.08 %
1
7.69 %
1
7.69 %
0
0.00 %
PAR-RAPID
4
11
0.03 %
3
27.27 %
8
72.73 %
1
9.09 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
130
0.32 %
4
3.08 %
121
93.08 %
10
7.69 %
5
3.85 %
0
0.00 %
Pembs
PAR-IR-EX
15
45
0.11 %
21
46.67 %
20
44.44 %
3
6.67 %
3
6.67 %
1
2.22 %
PAR-IR-SEV
33
196
0.48 %
76
38.78 %
97
49.49 %
28
14.29 %
12
6.12 %
11
5.61 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
14
0.03 %
5
35.71 %
8
57.14 %
1
7.14 %
1
7.14 %
0
0.00 %
PAR-PERSIS
10
650
1.58 %
64
9.85 %
538
82.77 %
59
9.08 %
45
6.92 %
3
0.46 %
PAR-RAPID
19
68
0.16 %
19
27.94 %
44
64.71 %
5
7.35 %
4
5.88 %
1
1.47 %
PAR-RECUR
8
702
1.70 %
48
6.84 %
584
83.19 %
61
8.69 %
46
6.55 %
24
3.42 %
SER-FLEXI
9
662
1.61 %
40
6.04 %
586
88.52 %
48
7.25 %
35
5.29 %
1
0.15 %
Powys
PAR-IR-EX
2
5
0.01 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-IR-SEV
11
87
0.21 %
14
16.09 %
63
72.41 %
12
13.79 %
9
10.34 %
1
1.15 %
PAR-NEW-IN
2
152
0.37 %
13
8.55 %
131
86.18 %
10
6.58 %
8
5.26 %
0
0.00 %
PAR-PERSIS
3
4
0.01 %
0
0.00 %
2
50.00 %
1
25.00 %
1
25.00 %
1
25.00 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
9
16
0.04 %
3
18.75 %
13
81.25 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
39
0.09 %
3
7.69 %
29
74.36 %
9
23.08 %
7
17.95 %
0
0.00 %
S Wales
PAR-IR-SEV
1
9
0.02 %
6
66.67 %
3
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
63
0.15 %
5
7.94 %
46
73.02 %
13
20.63 %
12
19.05 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.01 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
253
0.61 %
43
17.00 %
195
77.08 %
15
5.93 %
14
5.53 %
1
0.40 %
PAR-RECUR
5
397
0.96 %
4
1.01 %
368
92.70 %
21
5.29 %
20
5.04 %
5
1.26 %
Ceredigion
PAR-IR-EX
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
318
0.77 %
41
12.89 %
254
79.87 %
27
8.49 %
23
7.23 %
0
0.00 %
NE Wales
PAR-IR-EX
7
26
0.06 %
7
26.92 %
17
65.38 %
2
7.69 %
2
7.69 %
0
0.00 %
PAR-IR-SEV
3
32
0.08 %
6
18.75 %
25
78.13 %
1
3.13 %
1
3.13 %
0
0.00 %
PAR-NEW-IN
15
1,363
3.31 %
43
3.15 %
1184
86.87 %
137
10.05 %
123
9.02 %
13
0.95 %
PAR-PERSIS
4
35
0.08 %
1
2.86 %
28
80.00 %
9
25.71 %
6
17.14 %
0
0.00 %
PAR-POSTM
8
25
0.06 %
3
12.00 %
19
76.00 %
4
16.00 %
3
12.00 %
0
0.00 %
PAR-RAPID
8
11
0.03 %
4
36.36 %
7
63.64 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
56
0.14 %
1
1.79 %
50
89.29 %
6
10.71 %
5
8.93 %
0
0.00 %
SER-FLEXI
3
84
0.20 %
6
7.14 %
74
88.10 %
4
4.76 %
4
4.76 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-IR-SEV
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
2
92
0.22 %
3
3.26 %
78
84.78 %
12
13.04 %
11
11.96 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
122
0.30 %
0
0.00 %
113
92.62 %
12
9.84 %
9
7.38 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-PERSIS
2
5
0.01 %
0
0.00 %
5
100.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
33
2,436
5.91 %
94
3.86 %
2152
88.34 %
216
8.87 %
187
7.68 %
3
0.12 %
PAR-OTHER
2
11
0.03 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-PERSIS
1
7
0.02 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
5
5
0.01 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
291
0.71 %
10
3.44 %
267
91.75 %
18
6.19 %
13
4.47 %
1
0.34 %
NE Wales
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
28
2,897
7.03 %
67
2.31 %
2423
83.64 %
361
12.46 %
184
6.35 %
223
7.70 %
PAR-POSTM
2
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.02 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
4
165
0.40 %
12
7.27 %
138
83.64 %
17
10.30 %
15
9.09 %
0
0.00 %
NW Wales
PAR-NEW-IN
1
23
0.06 %
0
0.00 %
22
95.65 %
1
4.35 %
1
4.35 %
0
0.00 %
Wales
345
12730
30.87 %
887
6.97 %
10669
83.81 %
1213
9.53 %
878
6.90 %
296
2.33 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
4
470
1.14 %
42
8.94 %
378
80.43 %
50
10.64 %
45
9.57 %
5
1.06 %
West lothian
PAR-NEW-IN
5
438
1.06 %
25
5.71 %
320
73.06 %
37
8.45 %
36
8.22 %
57
13.01 %
Wigtown
PAR-NEW-IN
12
680
1.65 %
90
13.24 %
525
77.21 %
65
9.56 %
64
9.41 %
1
0.15 %
Scotland
21
1588
3.85 %
157
9.89 %
1223
77.02 %
152
9.57 %
145
9.13 %
63
3.97 %
Grand Total
Sum:
546
41234
2174
34761
3483
2846
1453
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol February 2023
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
93
11362
100.0%
555
4.9%
9874
86.9%
1094
9.6%
926
8.1%
7
0.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
93
11362
100.0%
555
4.9%
9874
86.9%
1094
9.6%
926
8.1%
7
0.1%
Scotland
Parallel
8
660
100.0%
63
9.5%
486
73.6%
68
10.3%
66
10.0%
45
6.8%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
8
660
100.0%
63
9.5%
486
73.6%
68
10.3%
66
10.0%
45
6.8%
Wales
Parallel
155
4428
80.3%
301
6.8%
3724
84.1%
421
9.5%
366
8.3%
37
0.8%
Serial/Extended
9
1088
19.7%
117
10.8%
928
85.3%
59
5.4%
40
3.7%
3
0.3%
Total
164
5516
100.0%
418
7.6%
4652
84.3%
480
8.7%
406
7.4%
40
0.7%
GB
Parallel
256
xx-xxxx-xxxx
93.8%
919
5.6%
14084
85.6%
1583
9.6%
1358
8.3%
89
0.5%
Serial/Extended
9
1088
6.2%
117
10.8%
928
85.3%
59
5.4%
40
3.7%
3
0.3%
Total
265
17538
100.0%
1036
5.9%
15012
85.6%
xx-xxxx-xxxx
9.4%
1398
8.0%
92
0.5%
93.00
xx-xxxx-xxxx
8.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2023)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
180
26916
100.0%
1130
4.2%
22869
85.0%
2118
7.9%
1823
6.8%
1094
4.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
180
26916
100.0%
1130
4.2%
22869
85.0%
2118
7.9%
1823
6.8%
1094
4.1%
Scotland
Parallel
21
1588
100.0%
157
9.9%
1223
77.0%
152
9.6%
145
9.1%
63
4.0%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
21
1588
100.0%
157
9.9%
1223
77.0%
152
9.6%
145
9.1%
63
4.0%
Wales
Parallel
319
11040
86.7%
721
6.5%
9236
83.7%
1095
9.9%
790
7.2%
293
2.7%
Serial/Extended
26
xx-xxxx-xxxx
13.3%
166
9.8%
1433
84.8%
118
7.0%
88
5.2%
3
0.2%
Total
345
12730
100.0%
887
7.0%
10669
83.8%
1213
9.5%
878
6.9%
296
2.3%
GB
Parallel
520
39544
95.9%
2008
5.1%
33328
84.3%
3365
8.5%
2758
7.0%
1450
3.7%
Serial/Extended
26
xx-xxxx-xxxx
4.1%
166
9.8%
1433
84.8%
118
7.0%
88
5.2%
3
0.2%
Total
546
41234
100.0%
2174
5.3%
34761
84.3%
3483
8.4%
2846
6.9%
1453
3.5%
180.00
345.00
21.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
1088
6.2%
117
10.8%
928
85.3%
59
5.4 %
40
3.7 %
3
0.3 %
Parallel - IRs in Exit Strategy for persistent her
15
50
0.3%
20
40.0%
27
54.0%
2
4.0 %
2
4.0 %
1
2.0 %
Parallel New Infection
77
5990
34.2%
215
3.6%
5214
87.0%
579
9.7 %
502
8.4 %
59
1.0 %
Parallel - new infection cull area
2
809
4.6%
60
7.4%
692
85.5%
67
8.3 %
57
7.0 %
0
Parallel - Other
4
588
3.4%
26
4.4%
542
92.2%
21
3.6 %
20
3.4 %
0
Parallel Persistent Infection
17
1748
10.0%
89
5.1%
1495
85.5%
186
10.6 %
163
9.3 %
1
0.1 %
Parallel – post-mvt test coupled with IDEXX an
8
15
0.1%
4
26.7%
10
66.7%
1
6.7 %
1
6.7 %
0
Parallel - Rapid Testing Two-times IRs
32
66
0.4%
21
31.8%
42
63.6%
2
3.0 %
2
3.0 %
1
1.5 %
Parallel - Recurrent breakdowns
68
6957
39.7%
415
6.0%
5919
85.1%
708
10.2 %
597
8.6 %
26
0.4 %
Parallel - severe IRs - in persistent herds
33
227
1.3%
69
30.4%
143
63.0%
17
7.5 %
14
6.2 %
1
0.4 %
Total
265
17538
100.0%
1036
5.9%
15012
85.6%
xx-xxxx-xxxx
9.4 %
1398
8.0 %
92
0.5 %
Table 4a. Summaryof IFN Gamma results by submission reason (February 2023)
England - HRA
Parallel - new infection cull area
2
809
4.6%
60
7.4%
692
85.5%
67
8.3 %
57
7.0 %
0
Parallel - Recurrent breakdowns
50
6121
34.9%
376
6.1%
5207
85.1%
635
10.4 %
534
8.7 %
4
0.1 %
England - HRA
52
6930
39.5%
436
6.3%
5899
85.1%
702
10.1 %
591
8.5 %
4
0.1 %
England - Edge
Parallel New Infection
20
1968
11.2%
64
3.3%
1764
89.6%
172
8.7 %
139
7.1 %
1
0.1 %
Parallel - Other
2
511
2.9%
16
3.1%
488
95.5%
7
1.4 %
7
1.4 %
0
Parallel Persistent Infection
8
1187
6.8%
32
2.7%
1040
87.6%
127
10.7 %
115
9.7 %
0
Parallel - Recurrent breakdowns
3
214
1.2%
4
1.9%
185
86.4%
26
12.1 %
24
11.2 %
1
0.5 %
England - Edge
33
3880
22.1%
116
3.0%
3477
89.6%
332
8.6 %
285
7.3 %
2
0.1 %
England - LRA
Parallel New Infection
7
511
2.9%
3
0.6%
457
89.4%
59
11.5 %
50
9.8 %
1
0.2 %
Parallel Persistent Infection
1
41
0.2%
0
0.0 %
41
100.0%
1
2.4 %
0
0
England - LRA
8
552
3.1%
3
0.5%
498
90.2%
60
10.9 %
50
9.1 %
1
0.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
8
1027
5.9%
111
10.8%
875
85.2%
57
5.6 %
38
3.7 %
3
0.3 %
Parallel - IRs in Exit Strategy for persistent her
11
39
0.2%
18
46.2%
18
46.2%
2
5.1 %
2
5.1 %
1
2.6 %
Parallel - Other
2
77
0.4%
10
13.0%
54
70.1%
14
18.2 %
13
16.9 %
0
Parallel Persistent Infection
5
191
1.1%
16
8.4%
151
79.1%
29
15.2 %
23
12.0 %
1
0.5 %
Parallel - Rapid Testing Two-times IRs
23
52
0.3%
20
38.5%
29
55.8%
2
3.8 %
2
3.8 %
1
1.9 %
Parallel - Recurrent breakdowns
7
476
2.7%
35
7.4%
389
81.7%
38
8.0 %
31
6.5 %
21
4.4 %
Parallel - severe IRs - in persistent herds
29
209
1.2%
62
29.7%
132
63.2%
17
8.1 %
14
6.7 %
1
0.5 %
Wales - High TB
85
2071
11.8%
272
13.1%
xx-xxxx-xxxx
79.6%
159
7.7 %
123
5.9 %
28
1.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
61
0.3%
6
9.8%
53
86.9%
2
3.3 %
2
3.3 %
0
Parallel - IRs in Exit Strategy for persistent her
4
11
0.1%
2
18.2%
9
81.8%
0
0
0
Parallel New Infection
11
885
5.0%
26
2.9%
774
87.5%
84
9.5 %
78
8.8 %
7
0.8 %
Parallel Persistent Infection
2
322
1.8%
41
12.7%
257
79.8%
28
8.7 %
24
7.5 %
0
Parallel – post-mvt test coupled with IDEXX an
3
9
0.1%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - Rapid Testing Two-times IRs
6
8
0.0%
1
12.5%
7
87.5%
0
0
0
Parallel - Recurrent breakdowns
5
133
0.8%
0
0.0 %
126
94.7%
8
6.0 %
7
5.3 %
0
Parallel - severe IRs - in persistent herds
3
17
0.1%
7
41.2%
10
58.8%
0
0
0
Wales - Intermediate TB
35
1446
8.2%
84
5.8%
1243
86.0%
123
8.5 %
112
7.7 %
7
0.5 %
Wales - Low TB
Parallel New Infection
31
1966
11.2%
59
3.0%
1733
88.1%
196
10.0 %
169
8.6 %
5
0.3 %
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX an
5
6
0.0%
3
50.0%
3
50.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
3
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Recurrent breakdowns
3
13
0.1%
0
0.0 %
12
92.3%
1
7.7 %
1
7.7 %
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
44
1999
11.4%
62
3.1%
1761
88.1%
198
9.9 %
171
8.6 %
5
0.3 %
Scotland - Scotland
Parallel New Infection
8
660
3.8%
63
9.5%
486
73.6%
68
10.3 %
66
10.0 %
45
6.8 %
Scotland - Scotland
8
660
3.8%
63
9.5%
486
73.6%
68
10.3 %
66
10.0 %
45
6.8 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
26
xx-xxxx-xxxx
4.1%
166
9.8%
1433
84.8%
118
7.0 %
88
5.2 %
3
0.2 %
Parallel - IRs in Exit Strategy for persistent herds
33
102
0.2%
41
40.2%
54
52.9%
7
6.9 %
6
5.9 %
1
1.0 %
Parallel New Infection
157
15737
38.2%
578
3.7%
13358
84.9%
1421
9.0 %
1103
7.0 %
698
4.4 %
Parallel - new infection cull area
3
811
2.0%
60
7.4%
693
85.5%
68
8.4 %
58
7.2 %
0
Parallel - Other
11
872
2.1%
70
8.0%
745
85.4%
39
4.5 %
37
4.2 %
20
2.3 %
Parallel Persistent Infection
34
2371
5.8%
138
5.8%
2023
85.3%
239
10.1 %
202
8.5 %
8
0.3 %
Parallel – post-mvt test coupled with IDEXX anti
16
34
0.1%
6
17.6%
25
73.5%
4
11.8 %
3
8.8 %
0
Parallel - Rapid Testing Two-times IRs
63
156
0.4%
47
30.1%
100
64.1%
10
6.4 %
7
4.5 %
2
1.3 %
Parallel - Recurrent breakdowns
138
19025
46.1%
909
4.8%
16095
84.6%
1528
8.0 %
1312
6.9 %
709
3.7 %
Parallel - severe IRs - in persistent herds
65
436
1.1%
159
36.5%
235
53.9%
49
11.2 %
30
6.9 %
12
2.8 %
Total
546
41234
100.0%
2174
5.3%
34761
84.3%
3483
8.4 %
2846
6.9 %
1453
3.5 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2023)
England - HRA
Parallel - new infection cull area
3
811
2.0%
60
7.4%
693
85.5%
68
8.4 %
58
7.2 %
0
Parallel - Recurrent breakdowns
92
15605
37.8%
769
4.9%
13087
83.9%
1254
8.0 %
1085
7.0 %
664
4.3 %
England - Edge
Parallel New Infection
36
4225
10.2%
118
2.8%
3717
88.0%
333
7.9 %
269
6.4 %
121
2.9 %
Parallel - Other
3
530
1.3%
16
3.0%
488
92.1%
7
1.3 %
7
1.3 %
19
3.6 %
Parallel Persistent Infection
8
1187
2.9%
32
2.7%
1040
87.6%
127
10.7 %
115
9.7 %
0
Parallel - Recurrent breakdowns
18
1556
3.8%
52
3.3%
1375
88.4%
129
8.3 %
114
7.3 %
15
1.0 %
England - LRA
Parallel New Infection
19
2961
7.2%
83
2.8%
2428
82.0%
199
6.7 %
175
5.9 %
275
9.3 %
Parallel Persistent Infection
1
41
0.1%
0
0.0 %
41
100.0%
1
2.4 %
0
0
England
180
26916
65.3%
1130
4.2%
22869
85.0%
2118
7.9 %
1823
6.8 %
1094
4.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
19
1441
3.5%
148
10.3%
1221
84.7%
97
6.7 %
69
4.8 %
3
0.2 %
Parallel - IRs in Exit Strategy for persistent herd
24
73
0.2%
33
45.2%
35
47.9%
4
5.5 %
4
5.5 %
1
1.4 %
Parallel New Infection
2
152
0.4%
13
8.6%
131
86.2%
10
6.6 %
8
5.3 %
0
Parallel - Other
2
77
0.2%
10
13.0%
54
70.1%
14
18.2 %
13
16.9 %
0
Parallel Persistent Infection
17
778
1.9%
64
8.2%
649
83.4%
73
9.4 %
57
7.3 %
8
1.0 %
Parallel – post-mvt test coupled with IDEXX ant
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel - Rapid Testing Two-times IRs
49
134
0.3%
43
32.1%
83
61.9%
9
6.7 %
6
4.5 %
2
1.5 %
Parallel - Recurrent breakdowns
15
998
2.4%
73
7.3%
835
83.7%
88
8.8 %
66
6.6 %
24
2.4 %
Parallel - severe IRs - in persistent herds
59
398
1.0%
149
37.4%
208
52.3%
48
12.1 %
29
7.3 %
12
3.0 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
84
0.2%
6
7.1%
74
88.1%
4
4.8 %
4
4.8 %
0
Parallel - IRs in Exit Strategy for persistent herd
8
28
0.1%
7
25.0%
19
67.9%
2
7.1 %
2
7.1 %
0
Parallel New Infection
17
1455
3.5%
46
3.2%
1262
86.7%
149
10.2 %
134
9.2 %
13
0.9 %
Parallel - Other
4
254
0.6%
43
16.9%
196
77.2%
15
5.9 %
14
5.5 %
1
0.4 %
Parallel Persistent Infection
7
358
0.9%
42
11.7%
287
80.2%
37
10.3 %
29
8.1 %
0
Parallel – post-mvt test coupled with IDEXX ant
8
25
0.1%
3
12.0%
19
76.0%
4
16.0 %
3
12.0 %
0
Parallel - Rapid Testing Two-times IRs
9
12
0.0%
4
33.3%
8
66.7%
0
0
0
Parallel - Recurrent breakdowns
8
575
1.4%
5
0.9%
531
92.3%
39
6.8 %
34
5.9 %
5
0.9 %
Parallel - severe IRs - in persistent herds
5
37
0.1%
10
27.0%
26
70.3%
1
2.7 %
1
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
165
0.4%
12
7.3%
138
83.6%
17
10.3 %
15
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
1
1
0.0%
1
100.0%
0
0.0 %
1
100.0 %
0
0
Parallel New Infection
62
5356
13.0%
161
3.0%
4597
85.8%
578
10.8 %
372
6.9 %
226
4.2 %
Parallel - Other
2
11
0.0%
1
9.1%
7
63.6%
3
27.3 %
3
27.3 %
0
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX ant
7
8
0.0%
3
37.5%
5
62.5%
0
0
0
Parallel - Rapid Testing Two-times IRs
5
10
0.0%
0
0.0 %
9
90.0%
1
10.0 %
1
10.0 %
0
Parallel - Recurrent breakdowns
5
291
0.7%
10
3.4%
267
91.8%
18
6.2 %
13
4.5 %
1
0.3 %
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
345
12730
30.9%
887
7.0%
10669
83.8%
1213
9.5 %
878
6.9 %
296
2.3 %
Scotland - Scotland
Parallel New Infection
21
1588
3.9%
157
9.9%
1223
77.0%
152
9.6 %
145
9.1 %
63
4.0 %
Scotland
21
1588
3.9%
157
9.9%
1223
77.0%
152
9.6 %
145
9.1 %
63
4.0 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2022 March
268
23046
165
1108
35
0
0
0
0
60
4
0
14
0
929
0
65
1
0
April
220
15067
130
687
9
0
0
0
0
50
2
0
11
0
563
0
52
0
0
May
269
23949
158
918
2
19
0
0
0
0
62
3
0
9
0
778
0
44
1
0
June
213
17755
114
590
19
0
0
0
0
48
1
0
2
0
460
1
57
2
0
July
248
15402
109
485
4
0
0
0
0
46
1
2
3
0
382
1
46
0
0
August
274
19758
144
758
12
0
0
0
0
36
3
0
14
0
642
0
47
1
2
September
205
xx-xxxx-xxxx
107
672
13
0
0
0
0
100
0
1
7
0
520
0
31
0
0
October
180
xx-xxxx-xxxx
100
620
12
0
0
0
0
66
0
0
21
0
495
0
26
0
0
November
201
15848
120
673
16
0
0
0
0
60
1
1
13
0
504
1
77
0
0
December
200
13735
109
545
37
0
0
0
0
26
0
0
8
3
425
2
28
0
11
2023 January
281
23696
176
1138
82
0
0
0
0
81
0
1
2
17
853
8
0
0
94
February
265
17538
147
1036
156
378
0
0
0
0
11
0
3
0
7
217
4
0
0
19
Totals (last
12 months)
2824
218634
1579
9230
158
636
0
0
0
0
646
15
8
104
27
6768
17
473
5
126
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1063
151325
715
5445
391
0
0
0
0
563
4
3
23
4
4230
4
14
0
0
Scotland
56
6015
36
283
4
0
0
0
0
56
1
4
6
12
142
4
5
0
10
Wales
1705
61294
828
3502
241
0
0
0
0
27
10
1
75
11
2396
9
454
5
116
Totals
2824
218634
1579
9230
636
0
0
0
0
646
15
8
104
27
6768
17
473
5
126
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2021
2022
2023
IFNg Positive herds
IFNg Negative herds
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2021
2022
2023
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2021
2022
2023
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
80
W Yorks
Wigtown
West lothian
S Wales
Staffs
Shropshire
Pembs
N Yorks
Northants
Leics & Rut
Hampshire
Gwent
Gloucs
Essex
Derbyshire
Corn & Scilly
Ceredigion
Bucks
Ayrshire
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during February 2023 (% submission per
County)
County
%Resampled %Retested
Avon
1.35 %
Ayrshire
11.11 %
11.47 %
Berks
7.38 %
8.00 %
Bucks
6.25 %
6.73 %
Carms
5.33 %
6.22 %
Ceredigion
5.43 %
7.24 %
Cheshire
7.18 %
7.89 %
Corn & Scilly
8.37 %
9.26 %
Cumbria
18.99 %
24.05 %
Derbyshire
10.05 %
11.03 %
Devon
6.69 %
8.69 %
Essex
1.14 %
2.27 %
E Sussex
10.88 %
13.39 %
Gloucs
13.54 %
16.67 %
Gr Manchester
2.44 %
Gwent
5.88 %
5.88 %
Gwynedd
9.75 %
10.51 %
Hampshire
5.05 %
7.80 %
Heref
12.29 %
14.13 %
Leics & Rut
4.16 %
6.03 %
NE Wales
8.00 %
9.23 %
Northants
35.71 %
42.86 %
Notts
6.45 %
9.68 %
N Yorks
2.50 %
2.50 %
Oxon
7.34 %
9.60 %
Pembs
5.60 %
7.54 %
Powys
8.33 %
9.65 %
Shropshire
9.49 %
12.62 %
Somerset excl
16.67 %
16.67 %
Staffs
18.87 %
19.36 %
Surrey
19.12 %
16.18 %
S Wales
Warks
12.00 %
12.00 %
West lothian
7.91 %
8.19 %
W Glamorgan
19.05 %
20.63 %

Wigtown
25.93 %
25.93 %
Wiltshire
2.47 %
3.85 %
W Yorks
5.80 %
7.25 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
11117
555
5.0%
11362
555
4.9%
Wales
5376
418
7.8%
5516
418
7.6%
Scotland
614
63
10.3%
660
63
9.5%
GB
17107
1036
6.1%
17538
1036
5.9%
Table 6a (i): Number of animals and submissions tested in February 2023
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
25417
1130
4.4%
26916
1130
4.2%
Wales
11949
887
7.4%
12730
887
7.0%
Scotland
1452
157
10.8%
1588
157
9.9%
GB
38816
2174
5.6%
41234
2174
5.3%
Table 6b (i): Number of animals and submissions tested in 2023 Up to end of February
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
14132
626
4.4%
14403
626
4.3%
Wales
3742
340
9.1%
3795
340
9.0%
GB
17874
966
5.4%
18198
966
5.3%
Table 6a (ii): Number of animals and submissions tested in February 2022
Table 6b (ii): Number of animals and submissions tested in 2022 Up to end of February
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
28296
1235
4.4%
29477
1235
4.2%
Wales
6969
583
8.4%
7282
583
8.0%
Scotland
2
0
0.0 %
2
0.0 %
GB
35267
1818
5.2%
36761
1818
4.9%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
2
0
0
Devon
PRV-SE
2
25
1
4.00 %
Oxfordshire
PRV-SP
2
2
0
0
Total
5
29
1
3.45 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples February 2023
Table 7b Number of private samples 2023
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
2
0
0
Devon
PRV-SE
3
86
4
4.65 %
Oxfordshire
PRV-SP
2
2
0
0
Shropshire
PRV-SP
2
2
0
0
Total
8
92
4
4.35 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
4
58
16
27.6%
BOV-IR-EX
4
11
2
18.2%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
3
25
6
24.0%
BOV-OTHER
1
80
2
2.5%
North East Wales
BOV-IR-C
2
13
2
15.4%
BOV-IR-EX
1
4
0
0
BOV-POSTM
4
7
2
28.6%
Pembrokeshire
BOV-IR-C
4
39
3
7.7%
BOV-IR-EX
4
9
1
11.1%
Powys
1
8
1
12.5%
BOV-IR-C
8
74
9
12.2%
South Wales
BOV-IR-C
2
23
5
21.7%
BOV-IR-EX
1
8
2
25.0%
Wales
40
408
52
12.7%
All GB
41
410
53
12.9 %
Table 8a Number of Cattle Antibody Tests February 2023
Table 8b Number of Cattle Antibody Tests 2023
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Northern Somerset &
South
Gloucestershire
BOV-P
1
5
0
0
England
1
5
0
0
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
6
90
25
27.8%
BOV-IR-EX
4
11
2
18.2%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
6
46
10
21.7%
BOV-IR-EX
1
3
0
0
BOV-OTHER
1
80
2
2.5%
North East Wales
BOV-IR-C
2
13
2
15.4%
BOV-IR-EX
2
11
0
0
BOV-P
1
14
0
0
BOV-POSTM
7
20
4
20.0%
Pembrokeshire
BOV-IR-C
22
231
25
10.8%
BOV-IR-EX
6
40
6
15.0%
BOV-OTHER
3
328
6
1.8%
Powys
1
8
1
12.5%
BOV-IR-C
12
90
11
12.2%
South Wales
BOV-IR-C
2
23
5
21.7%
BOV-IR-EX
1
8
2
25.0%
Wales
78
1,065
102
9.6%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
All GB
80
1,072
103
9.6 %