Survellience Project SB4008 IGNg tests for Bovine tuberculosis - October 2018
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 146
Report Period 1st - 31st October 2018
Issued 13 November 2018

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
October
2018
%
Total 2018
%
Submissions
274
2159
Samples
Total (%)
23976
100.00 %
207362
100.00 %
England
19262
80.34 %
160008
77.16 %
Wales
4699
19.60 %
45113
21.76 %
Scotland
15
0.06 %
2241
1.08 %
Parallel Tests
PAR-CUL-N
4511
18.81 %
24638
11.88 %
PAR-CUL-P
596
2.49 %
6155
2.97 %
PAR-HERD-S
423
1.76 %
5696
2.75 %
PAR-NEW-IN
11297
47.12 %
109509
52.81 %
PAR-OTHER
1235
5.15 %
2585
1.25 %
PAR-PERSIS
4932
20.57 %
49364
23.81 %
PAR-RAPID
163
0.68 %
1315
0.63 %
Total(% of all samples)
23157
96.58 %
199262
96.09 %
Serial/Extended Tests
SER-FLEXI
809
3.37 %
7801
3.76 %
SER-FRAUD
0
0.00 %
270
0.13 %
SER-NSR
10
0.04 %
29
0.01 %
SER-OTHER
0
0.00 %
Total(% of all samples)
819
3.42 %
8100
3.91 %
Total
23976
100.00 %
207362
100.00 %
Retests
Total (% of all samples)
1860
7.76 %
16682
8.04 %
England
1562
6.51 %
12756
6.15 %
Wales
297
1.24 %
3725
1.80 %
Scotland
1
0.00 %
201
0.10 %
Resamples
Total (% of all samples)
1145
4.78 %
11573
5.58 %
England
974
4.06 %
8723
4.21 %
Wales
170
0.71 %
2711
1.31 %
Scotland
1
0.00 %
139
0.07 %
Rejects
Total (% of all samples)
56
0.23 %
1923
0.93 %
England
11
0.05 %
787
0.38 %
Wales
45
0.19 %
1136
0.55 %
Scotland
0
0.00 %
Table 1. Monthly Totals for October 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CUL-N
4511
18.8 %
PAR-CUL-P
596
2.5 %
PAR-HERD-S
423
1.8 %
PAR-NEW-IN
11297
47.1 %
PAR-OTHER
1235
5.2 %
PAR-PERSIS
4932
20.6 %
PAR-RAPID
163
0.7 %
SER-FLEXI
809
3.4 %
SER-NSR
10
0.0 %
Total
23976
100.00 %
Figure 1. Number of samples submitted in each category during October 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
Aberdeenshire
W Glamorgan
Shropshire
Powys
Pembs
NE Wales
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Somerset
Shropshire
Oxon
Notts
Northants
Leics & Rut
Kent
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during October 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
Wigtown
West lothian
Perthshire
Ayrshire
Argyll & Bute
Aberdeenshire
W Glamorgan
S Wales
Shropshire
Powys
Pembs
Pembrokshire
NE Wales
Mid glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
W Midlands
Wiltshire
Warks
S Yorks
Staffs
Somerset
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Merseyside
Lincs
Leics & Rut
Kent
Isle of wight
Humberside
Herts
Heref
Hampshire
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Table 2a. Summary by County for October 2018
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
8
0.03 %
0
0.00 %
4
50.00 %
4
50.00 %
4
50.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
13
1,166
4.86 %
35
3.00 %
1054
90.39 %
101
8.66 %
77
6.60 %
0
0.00 %
PAR-PERSIS
1
14
0.06 %
0
0.00 %
14
100.00 %
2
14.29 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
9
2,042
8.52 %
171
8.37 %
1778
87.07 %
163
7.98 %
90
4.41 %
3
0.15 %
PAR-CUL-P
1
265
1.11 %
6
2.26 %
255
96.23 %
6
2.26 %
4
1.51 %
0
0.00 %
PAR-NEW-IN
2
548
2.29 %
29
5.29 %
505
92.15 %
19
3.47 %
14
2.55 %
0
0.00 %
PAR-PERSIS
3
745
3.11 %
31
4.16 %
692
92.89 %
48
6.44 %
22
2.95 %
0
0.00 %
Dorset
PAR-CUL-N
3
319
1.33 %
21
6.58 %
287
89.97 %
11
3.45 %
9
2.82 %
2
0.63 %
PAR-CUL-P
1
271
1.13 %
13
4.80 %
239
88.19 %
26
9.59 %
19
7.01 %
0
0.00 %
PAR-OTHER
2
98
0.41 %
7
7.14 %
80
81.63 %
15
15.31 %
11
11.22 %
0
0.00 %
SER-FLEXI
1
69
0.29 %
4
5.80 %
62
89.86 %
3
4.35 %
3
4.35 %
0
0.00 %
Gloucs
PAR-CUL-N
4
512
2.14 %
22
4.30 %
460
89.84 %
35
6.84 %
30
5.86 %
0
0.00 %
PAR-CUL-P
3
60
0.25 %
0
0.00 %
57
95.00 %
9
15.00 %
3
5.00 %
0
0.00 %
PAR-PERSIS
4
617
2.57 %
57
9.24 %
540
87.52 %
56
9.08 %
20
3.24 %
0
0.00 %
SER-FLEXI
2
198
0.83 %
16
8.08 %
179
90.40 %
14
7.07 %
2
1.01 %
1
0.51 %
Heref
PAR-CUL-N
3
196
0.82 %
9
4.59 %
173
88.27 %
17
8.67 %
14
7.14 %
0
0.00 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-OTHER
5
1,117
4.66 %
64
5.73 %
1015
90.87 %
51
4.57 %
37
3.31 %
1
0.09 %
Somerset
PAR-CUL-N
5
276
1.15 %
9
3.26 %
255
92.39 %
13
4.71 %
10
3.62 %
2
0.72 %
Wiltshire
PAR-NEW-IN
1
343
1.43 %
33
9.62 %
300
87.46 %
25
7.29 %
10
2.92 %
0
0.00 %
PAR-PERSIS
3
1,036
4.32 %
51
4.92 %
958
92.47 %
36
3.47 %
27
2.61 %
0
0.00 %
SER-FLEXI
2
48
0.20 %
5
10.42 %
40
83.33 %
4
8.33 %
3
6.25 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
51
0.21 %
0
0.00 %
50
98.04 %
1
1.96 %
1
1.96 %
0
0.00 %
Bucks
PAR-NEW-IN
2
24
0.10 %
5
20.83 %
17
70.83 %
3
12.50 %
2
8.33 %
0
0.00 %
Cheshire
PAR-NEW-IN
25
2,744
11.44 %
131
4.77 %
2385
86.92 %
327
11.92 %
228
8.31 %
0
0.00 %
PAR-PERSIS
1
11
0.05 %
0
0.00 %
8
72.73 %
3
27.27 %
3
27.27 %
0
0.00 %
Derbyshire
PAR-NEW-IN
22
3,094
12.90 %
123
3.98 %
2799
90.47 %
307
9.92 %
172
5.56 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
84
0.35 %
10
11.90 %
69
82.14 %
7
8.33 %
5
5.95 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-NSR
1
10
0.04 %
0
0.00 %
10
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
7
292
1.22 %
28
9.59 %
257
88.01 %
17
5.82 %
7
2.40 %
0
0.00 %
Northants
PAR-NEW-IN
2
120
0.50 %
8
6.67 %
107
89.17 %
10
8.33 %
5
4.17 %
0
0.00 %
Notts
PAR-NEW-IN
2
59
0.25 %
4
6.78 %
49
83.05 %
7
11.86 %
6
10.17 %
0
0.00 %
Oxon
PAR-NEW-IN
9
1,519
6.34 %
110
7.24 %
1322
87.03 %
134
8.82 %
87
5.73 %
0
0.00 %
Warks
PAR-NEW-IN
4
228
0.95 %
10
4.39 %
211
92.54 %
9
3.95 %
5
2.19 %
2
0.88 %
England - LRA
Cumbria
PAR-NEW-IN
3
409
1.71 %
9
2.20 %
378
92.42 %
36
8.80 %
22
5.38 %
0
0.00 %
Kent
PAR-NEW-IN
3
649
2.71 %
33
5.08 %
599
92.30 %
37
5.70 %
17
2.62 %
0
0.00 %
W Sussex
PAR-NEW-IN
1
18
0.08 %
0
0.00 %
13
72.22 %
6
33.33 %
5
27.78 %
0
0.00 %
England
157
19262
80.34 %
1054
5.47 %
17223
89.41 %
1562
8.11 %
974
5.06 %
11
0.06 %
Wales - High TB
Carms
PAR-HERD-S
3
355
1.48 %
19
5.35 %
328
92.39 %
13
3.66 %
8
2.25 %
0
0.00 %
PAR-PERSIS
8
792
3.30 %
76
9.60 %
677
85.48 %
54
6.82 %
38
4.80 %
1
0.13 %
PAR-RAPID
9
66
0.28 %
9
13.64 %
25
37.88 %
5
7.58 %
3
4.55 %
29
43.94 %
SER-FLEXI
4
119
0.50 %
13
10.92 %
97
81.51 %
6
5.04 %
5
4.20 %
4
3.36 %
Ceredigion
PAR-OTHER
1
9
0.04 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-RAPID
3
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-HERD-S
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
209
0.87 %
39
18.66 %
161
77.03 %
43
20.57 %
9
4.31 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
10
753
3.14 %
49
6.51 %
679
90.17 %
26
3.45 %
19
2.52 %
6
0.80 %
PAR-RAPID
18
68
0.28 %
22
32.35 %
43
63.24 %
4
5.88 %
3
4.41 %
0
0.00 %
SER-FLEXI
10
285
1.19 %
27
9.47 %
245
85.96 %
15
5.26 %
12
4.21 %
1
0.35 %
Powys
PAR-HERD-S
1
8
0.03 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
163
0.68 %
11
6.75 %
143
87.73 %
12
7.36 %
7
4.29 %
2
1.23 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
5
642
2.68 %
60
9.35 %
557
86.76 %
40
6.23 %
25
3.89 %
0
0.00 %
PAR-RAPID
2
10
0.04 %
1
10.00 %
9
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Ceredigion
PAR-HERD-S
1
54
0.23 %
0
0.00 %
51
94.44 %
6
11.11 %
3
5.56 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
10
578
2.41 %
12
2.08 %
550
95.16 %
38
6.57 %
16
2.77 %
0
0.00 %
PAR-PERSIS
1
101
0.42 %
3
2.97 %
91
90.10 %
8
7.92 %
7
6.93 %
0
0.00 %
PAR-RAPID
3
9
0.04 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
82
0.34 %
7
8.54 %
71
86.59 %
5
6.10 %
4
4.88 %
0
0.00 %
Powys
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
11
0.05 %
5
45.45 %
6
54.55 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
NE Wales
PAR-NEW-IN
3
354
1.48 %
4
1.13 %
340
96.05 %
19
5.37 %
8
2.26 %
2
0.56 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
115
4699
19.60 %
363
7.73 %
4121
87.70 %
297
6.32 %
170
3.62 %
45
0.96 %
Scotland - Scotland
Aberdeenshire
PAR-NEW-IN
2
15
0.06 %
0
0.00 %
14
93.33 %
1
6.67 %
1
6.67 %
0
0.00 %
Scotland
2
15
0.06 %
0
0.00 %
14
93.33 %
1
6.67 %
1
6.67 %
0
0.00 %
Grand Total
Sum:
274
23976
1417
21358
1860
1145
56
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
4
0.00 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-PERSIS
8
995
0.48 %
59
5.93 %
849
85.33 %
104
10.45 %
86
8.64 %
1
0.10 %
SER-FLEXI
2
401
0.19 %
55
13.72 %
309
77.06 %
43
10.72 %
37
9.23 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
36
5,507
2.66 %
226
4.10 %
5014
91.05 %
406
7.37 %
267
4.85 %
0
0.00 %
PAR-CUL-P
1
10
0.00 %
0
0.00 %
8
80.00 %
4
40.00 %
2
20.00 %
0
0.00 %
PAR-NEW-IN
1
121
0.06 %
18
14.88 %
98
80.99 %
14
11.57 %
5
4.13 %
0
0.00 %
PAR-OTHER
3
228
0.11 %
6
2.63 %
209
91.67 %
21
9.21 %
13
5.70 %
0
0.00 %
PAR-PERSIS
22
4,178
2.01 %
239
5.72 %
3783
90.55 %
240
5.74 %
156
3.73 %
0
0.00 %
SER-FLEXI
9
1,179
0.57 %
96
8.14 %
1028
87.19 %
54
4.58 %
47
3.99 %
8
0.68 %
Devon
PAR-CUL-N
42
6,524
3.15 %
464
7.11 %
5627
86.25 %
524
8.03 %
324
4.97 %
109
1.67 %
PAR-CUL-P
18
5,214
2.51 %
301
5.77 %
4621
88.63 %
287
5.50 %
192
3.68 %
100
1.92 %
PAR-NEW-IN
9
904
0.44 %
67
7.41 %
785
86.84 %
62
6.86 %
40
4.42 %
12
1.33 %
PAR-OTHER
5
36
0.02 %
1
2.78 %
30
83.33 %
5
13.89 %
5
13.89 %
0
0.00 %
PAR-PERSIS
34
6,090
2.94 %
314
5.16 %
5348
87.82 %
537
8.82 %
414
6.80 %
14
0.23 %
SER-FLEXI
14
2,191
1.06 %
160
7.30 %
1967
89.78 %
162
7.39 %
61
2.78 %
3
0.14 %
Dorset
PAR-CUL-N
46
6,699
3.23 %
380
5.67 %
5935
88.60 %
498
7.43 %
381
5.69 %
3
0.04 %
PAR-CUL-P
1
271
0.13 %
13
4.80 %
239
88.19 %
26
9.59 %
19
7.01 %
0
0.00 %
PAR-NEW-IN
2
636
0.31 %
8
1.26 %
558
87.74 %
73
11.48 %
70
11.01 %
0
0.00 %
PAR-OTHER
2
98
0.05 %
7
7.14 %
80
81.63 %
15
15.31 %
11
11.22 %
0
0.00 %
PAR-PERSIS
13
5,769
2.78 %
263
4.56 %
5238
90.80 %
392
6.79 %
267
4.63 %
1
0.02 %
SER-FLEXI
1
69
0.03 %
4
5.80 %
62
89.86 %
3
4.35 %
3
4.35 %
0
0.00 %
Gloucs
PAR-CUL-N
39
4,097
1.98 %
149
3.64 %
3659
89.31 %
477
11.64 %
286
6.98 %
3
0.07 %
PAR-CUL-P
9
560
0.27 %
21
3.75 %
471
84.11 %
92
xx-xxxx-xxxx %
68
12.14 %
0
0.00 %
PAR-NEW-IN
2
186
0.09 %
5
2.69 %
160
86.02 %
27
14.52 %
21
11.29 %
0
0.00 %
PAR-OTHER
2
180
0.09 %
30
16.67 %
141
78.33 %
17
9.44 %
9
5.00 %
0
0.00 %
PAR-PERSIS
4
617
0.30 %
57
9.24 %
540
87.52 %
56
9.08 %
20
3.24 %
0
0.00 %
SER-FLEXI
5
1,342
0.65 %
45
3.35 %
1247
92.92 %
76
5.66 %
49
3.65 %
1
0.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Heref
PAR-CUL-N
22
749
0.36 %
60
8.01 %
630
84.11 %
78
10.41 %
59
7.88 %
0
0.00 %
PAR-CUL-P
2
78
0.04 %
3
3.85 %
72
92.31 %
4
5.13 %
3
3.85 %
0
0.00 %
PAR-NEW-IN
3
466
0.22 %
25
5.36 %
429
92.06 %
39
8.37 %
12
2.58 %
0
0.00 %
PAR-PERSIS
4
232
0.11 %
14
6.03 %
207
89.22 %
11
4.74 %
10
4.31 %
1
0.43 %
SER-FLEXI
2
246
0.12 %
35
14.23 %
200
81.30 %
27
10.98 %
11
4.47 %
0
0.00 %
SER-FRAUD
2
38
0.02 %
1
2.63 %
36
94.74 %
2
5.26 %
1
2.63 %
0
0.00 %
Shropshire
PAR-OTHER
7
1,130
0.54 %
64
5.66 %
1027
90.88 %
52
4.60 %
38
3.36 %
1
0.09 %
PAR-PERSIS
12
1,613
0.78 %
125
7.75 %
1435
88.96 %
83
5.15 %
53
3.29 %
0
0.00 %
Somerset
PAR-CUL-N
19
1,062
0.51 %
50
4.71 %
910
85.69 %
57
5.37 %
50
4.71 %
52
4.90 %
PAR-CUL-P
1
22
0.01 %
1
4.55 %
17
77.27 %
6
27.27 %
4
18.18 %
0
0.00 %
PAR-NEW-IN
1
22
0.01 %
1
4.55 %
16
72.73 %
5
22.73 %
5
22.73 %
0
0.00 %
PAR-OTHER
5
593
0.29 %
40
6.75 %
423
71.33 %
24
4.05 %
22
3.71 %
108
18.21 %
PAR-PERSIS
21
4,736
2.28 %
413
8.72 %
4098
86.53 %
299
6.31 %
204
4.31 %
21
0.44 %
Staffs
PAR-PERSIS
3
640
0.31 %
17
2.66 %
588
91.88 %
33
5.16 %
18
2.81 %
17
2.66 %
SER-FLEXI
2
400
0.19 %
41
10.25 %
337
84.25 %
22
5.50 %
22
5.50 %
0
0.00 %
Wiltshire
PAR-NEW-IN
1
343
0.17 %
33
9.62 %
300
87.46 %
25
7.29 %
10
2.92 %
0
0.00 %
PAR-OTHER
2
46
0.02 %
13
28.26 %
31
67.39 %
2
4.35 %
2
4.35 %
0
0.00 %
PAR-PERSIS
11
1,701
0.82 %
70
4.12 %
1582
93.00 %
72
4.23 %
49
2.88 %
0
0.00 %
SER-FLEXI
2
48
0.02 %
5
10.42 %
40
83.33 %
4
8.33 %
3
6.25 %
0
0.00 %
SER-FRAUD
2
9
0.00 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Midlands
PAR-PERSIS
1
281
0.14 %
1
0.36 %
265
94.31 %
16
5.69 %
14
4.98 %
1
0.36 %
SER-FRAUD
3
120
0.06 %
2
1.67 %
111
92.50 %
10
8.33 %
7
5.83 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
35
3,087
1.49 %
148
4.79 %
2740
88.76 %
347
11.24 %
199
6.45 %
0
0.00 %
PAR-PERSIS
6
105
0.05 %
6
5.71 %
95
90.48 %
7
6.67 %
4
3.81 %
0
0.00 %
Bucks
PAR-NEW-IN
19
2,624
1.27 %
121
4.61 %
2271
86.55 %
143
5.45 %
99
3.77 %
133
5.07 %
Cheshire
PAR-NEW-IN
170
32,960
15.89 %
1148
3.48 %
30109
91.35 %
2467
7.48 %
1702
5.16 %
1
0.00 %
PAR-PERSIS
10
2,218
1.07 %
139
6.27 %
1770
79.80 %
235
10.60 %
207
9.33 %
102
4.60 %
Derbyshire
PAR-NEW-IN
94
12,293
5.93 %
419
3.41 %
11110
90.38 %
1122
9.13 %
761
6.19 %
3
0.02 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
E Sussex
PAR-NEW-IN
24
2,904
1.40 %
127
4.37 %
2615
90.05 %
270
9.30 %
160
5.51 %
2
0.07 %
Hampshire
PAR-NEW-IN
30
2,759
1.33 %
103
3.73 %
2508
90.90 %
213
7.72 %
148
5.36 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-PERSIS
1
19
0.01 %
1
5.26 %
16
84.21 %
7
36.84 %
2
10.53 %
0
0.00 %
SER-NSR
3
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
40
3,657
1.76 %
167
4.57 %
3242
88.65 %
342
9.35 %
246
6.73 %
2
0.05 %
SER-NSR
1
13
0.01 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
27
1,534
0.74 %
79
5.15 %
1372
89.44 %
124
8.08 %
83
5.41 %
0
0.00 %
Notts
PAR-NEW-IN
19
1,498
0.72 %
51
3.40 %
1336
89.19 %
150
10.01 %
111
7.41 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
85
8,950
4.32 %
541
6.04 %
7832
87.51 %
846
9.45 %
568
6.35 %
9
0.10 %
PAR-PERSIS
8
1,643
0.79 %
63
3.83 %
1508
91.78 %
82
4.99 %
72
4.38 %
0
0.00 %
Warks
PAR-NEW-IN
68
5,384
2.60 %
260
4.83 %
4717
87.61 %
496
9.21 %
331
6.15 %
76
1.41 %
PAR-PERSIS
2
23
0.01 %
1
4.35 %
19
82.61 %
3
13.04 %
3
13.04 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
561
0.27 %
12
2.14 %
453
80.75 %
124
22.10 %
96
17.11 %
0
0.00 %
Cumbria
PAR-NEW-IN
31
3,438
1.66 %
85
2.47 %
3180
92.50 %
218
6.34 %
173
5.03 %
0
0.00 %
PAR-PERSIS
1
330
0.16 %
11
3.33 %
304
92.12 %
18
5.45 %
15
4.55 %
0
0.00 %
Essex
PAR-NEW-IN
3
493
0.24 %
31
6.29 %
456
92.49 %
26
5.27 %
3
0.61 %
3
0.61 %
Herts
PAR-NEW-IN
4
366
0.18 %
5
1.37 %
325
88.80 %
61
16.67 %
36
9.84 %
0
0.00 %
Humberside
PAR-NEW-IN
2
99
0.05 %
8
8.08 %
86
86.87 %
8
8.08 %
5
5.05 %
0
0.00 %
Isle of wight
PAR-NEW-IN
2
146
0.07 %
8
5.48 %
115
78.77 %
35
23.97 %
23
15.75 %
0
0.00 %
Kent
PAR-NEW-IN
3
649
0.31 %
33
5.08 %
599
92.30 %
37
5.70 %
17
2.62 %
0
0.00 %
Lincs
PAR-NEW-IN
8
252
0.12 %
12
4.76 %
216
85.71 %
32
12.70 %
24
9.52 %
0
0.00 %
Merseyside
PAR-NEW-IN
2
34
0.02 %
0
0.00 %
20
58.82 %
15
44.12 %
14
41.18 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
455
0.22 %
9
1.98 %
409
89.89 %
48
10.55 %
37
8.13 %
0
0.00 %
N Yorks
PAR-NEW-IN
6
942
0.45 %
30
3.18 %
866
91.93 %
79
8.39 %
46
4.88 %
0
0.00 %
S Yorks
PAR-NEW-IN
2
131
0.06 %
11
8.40 %
110
83.97 %
10
7.63 %
10
7.63 %
0
0.00 %
W Sussex
PAR-NEW-IN
11
1,442
0.70 %
81
5.62 %
1300
90.15 %
87
6.03 %
61
4.23 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
297
0.14 %
6
2.02 %
277
93.27 %
15
5.05 %
14
4.71 %
0
0.00 %
England
1188
160008
77.16 %
7718
4.82 % ######
89.23 %
12756
7.97 %
8723
5.45 %
787
0.49 %
Wales - High TB
Carms
PAR-HERD-S
29
1,173
0.57 %
76
6.48 %
1042
88.83 %
79
6.73 %
53
4.52 %
2
0.17 %
PAR-OTHER
6
53
0.03 %
6
11.32 %
46
86.79 %
2
3.77 %
1
1.89 %
0
0.00 %
PAR-PERSIS
42
3,862
1.86 %
315
8.16 %
3335
86.35 %
279
7.22 %
206
5.33 %
6
0.16 %
PAR-RAPID
85
396
0.19 %
90
22.73 %
258
65.15 %
27
6.82 %
19
4.80 %
29
7.32 %
SER-FLEXI
10
342
0.16 %
35
10.23 %
255
74.56 %
56
16.37 %
48
14.04 %
4
1.17 %
Ceredigion
PAR-HERD-S
2
6
0.00 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
PAR-OTHER
1
9
0.00 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
16
1,300
0.63 %
91
7.00 %
1147
88.23 %
70
5.38 %
58
4.46 %
4
0.31 %
PAR-RAPID
28
94
0.05 %
21
22.34 %
73
77.66 %
3
3.19 %
0
0.00 %
0
0.00 %
SER-FLEXI
8
249
0.12 %
24
9.64 %
200
80.32 %
29
11.65 %
22
8.84 %
3
1.20 %
Gwent
PAR-HERD-S
5
429
0.21 %
16
3.73 %
395
92.07 %
36
8.39 %
16
3.73 %
2
0.47 %
PAR-NEW-IN
2
17
0.01 %
1
5.88 %
13
76.47 %
4
23.53 %
3
17.65 %
0
0.00 %
PAR-OTHER
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
37
2,270
1.09 %
118
5.20 %
1979
87.18 %
304
13.39 %
170
7.49 %
3
0.13 %
PAR-RAPID
32
170
0.08 %
28
xx-xxxx-xxxx %
128
75.29 %
14
8.24 %
14
8.24 %
0
0.00 %
SER-FLEXI
6
205
0.10 %
3
1.46 %
179
87.32 %
37
18.05 %
23
11.22 %
0
0.00 %
Pembrokshire
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Pembs
PAR-HERD-S
29
2,521
1.22 %
53
2.10 %
1989
78.90 %
160
6.35 %
115
4.56 %
364
14.44 %
PAR-OTHER
4
34
0.02 %
7
20.59 %
27
79.41 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
77
6,439
3.11 %
236
3.67 %
5586
86.75 %
498
7.73 %
359
5.58 %
258
4.01 %
PAR-RAPID
121
394
0.19 %
93
23.60 %
283
71.83 %
23
5.84 %
18
4.57 %
0
0.00 %
SER-FLEXI
24
768
0.37 %
43
5.60 %
609
79.30 %
69
8.98 %
51
6.64 %
65
8.46 %
SER-FRAUD
2
100
0.05 %
55
55.00 %
45
45.00 %
1
1.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
14
658
0.32 %
73
11.09 %
546
82.98 %
47
7.14 %
38
5.78 %
1
0.15 %
PAR-NEW-IN
22
852
0.41 %
20
2.35 %
797
93.54 %
48
5.63 %
33
3.87 %
2
0.23 %
PAR-OTHER
5
109
0.05 %
3
2.75 %
103
94.50 %
4
3.67 %
3
2.75 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
43
3,626
1.75 %
129
3.56 %
3111
85.80 %
259
7.14 %
213
5.87 %
173
4.77 %
PAR-RAPID
31
111
0.05 %
14
12.61 %
85
76.58 %
12
10.81 %
11
9.91 %
1
0.90 %
SER-FLEXI
6
101
0.05 %
3
2.97 %
93
92.08 %
5
4.95 %
5
4.95 %
0
0.00 %
S Wales
PAR-PERSIS
2
7
0.00 %
1
14.29 %
4
57.14 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
3
8
0.00 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-PERSIS
1
8
0.00 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
11
0.01 %
4
36.36 %
7
63.64 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
8
891
0.43 %
6
0.67 %
722
81.03 %
216
24.24 %
163
18.29 %
0
0.00 %
PAR-NEW-IN
1
68
0.03 %
4
5.88 %
63
92.65 %
3
4.41 %
1
1.47 %
0
0.00 %
PAR-PERSIS
3
153
0.07 %
22
14.38 %
116
75.82 %
24
15.69 %
15
9.80 %
0
0.00 %
PAR-RAPID
19
86
0.04 %
20
23.26 %
63
73.26 %
4
4.65 %
3
3.49 %
0
0.00 %
SER-FLEXI
4
117
0.06 %
3
2.56 %
107
91.45 %
8
6.84 %
7
5.98 %
0
0.00 %
Mid glamorgan
PAR-PERSIS
2
106
0.05 %
2
1.89 %
80
75.47 %
30
28.30 %
24
22.64 %
0
0.00 %
NE Wales
PAR-HERD-S
1
17
0.01 %
0
0.00 %
13
76.47 %
4
23.53 %
4
23.53 %
0
0.00 %
PAR-NEW-IN
115
11,334
5.47 %
190
1.68 %
10435
92.07 %
871
7.68 %
651
5.74 %
58
0.51 %
PAR-PERSIS
1
101
0.05 %
3
2.97 %
91
90.10 %
8
7.92 %
7
6.93 %
0
0.00 %
PAR-RAPID
9
21
0.01 %
3
14.29 %
17
80.95 %
0
0.00 %
0
0.00 %
1
4.76 %
SER-FLEXI
4
82
0.04 %
7
8.54 %
71
86.59 %
5
6.10 %
4
4.88 %
0
0.00 %
Powys
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
PAR-NEW-IN
8
398
0.19 %
15
3.77 %
364
91.46 %
38
9.55 %
19
4.77 %
0
0.00 %
PAR-RAPID
2
7
0.00 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
2
21
0.01 %
1
4.76 %
19
90.48 %
4
19.05 %
1
4.76 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
11
0.01 %
5
45.45 %
6
54.55 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
5
0.00 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
14
867
0.42 %
8
0.92 %
786
90.66 %
99
11.42 %
72
8.30 %
1
0.12 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
35
4,431
2.14 %
92
2.08 %
3930
88.69 %
334
7.54 %
251
5.66 %
158
3.57 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
5
0.00 %
0
0.00 %
2
40.00 %
3
60.00 %
3
60.00 %
0
0.00 %
PAR-RAPID
3
14
0.01 %
2
14.29 %
12
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
46
0.02 %
0
0.00 %
45
97.83 %
2
4.35 %
1
2.17 %
0
0.00 %
Wales
940
45113
21.76 %
1950
4.32 %
39316
87.15 %
3725
8.26 %
2711
6.01 %
1136
2.52 %
Scotland - Scotland
Aberdeenshire
PAR-NEW-IN
2
15
0.01 %
0
0.00 %
14
93.33 %
1
6.67 %
1
6.67 %
0
0.00 %
Argyll & Bute
PAR-NEW-IN
12
887
0.43 %
68
7.67 %
766
86.36 %
77
8.68 %
53
5.98 %
0
0.00 %
SER-FLEXI
1
10
0.00 %
1
10.00 %
8
80.00 %
1
10.00 %
1
10.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
7
579
0.28 %
37
6.39 %
496
85.66 %
66
11.40 %
46
7.94 %
0
0.00 %
PAR-OTHER
1
46
0.02 %
39
84.78 %
7
15.22 %
1
2.17 %
0
0.00 %
0
0.00 %
Perthshire
PAR-NEW-IN
3
217
0.10 %
17
7.83 %
187
86.18 %
22
10.14 %
13
5.99 %
0
0.00 %
West lothian
PAR-NEW-IN
2
142
0.07 %
1
0.70 %
137
96.48 %
4
2.82 %
4
2.82 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
345
0.17 %
18
5.22 %
306
88.70 %
29
8.41 %
21
6.09 %
0
0.00 %
Scotland
31
2241
1.08 %
181
8.08 %
1921
85.72 %
201
8.97 %
139
6.20 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
9849
######
16682
11573
1923
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol October 2018
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
151
18937
98.3%
1029
5.4%
xx-xxxx-xxxx
89.4%
1541
8.1%
966
5.1%
10
0.1%
Serial/Extended
6
325
1.7%
25
7.7%
291
89.5%
21
6.5%
8
2.5%
1
0.3%
Total
157
19262
100.0%
1054
5.5%
17223
89.4%
1562
8.1%
974
5.1%
11
0.1%
Scotland
Parallel
2
15
100.0%
0
0.0 %
14
93.3%
1
6.7%
1
6.7%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
15
100.0%
0
0.0 %
14
93.3%
1
6.7%
1
6.7%
0
0.0 %
Wales
Parallel
94
4205
89.5%
316
7.5%
3701
88.0%
270
6.4%
148
3.5%
40
1.0%
Serial/Extended
21
494
10.5%
47
9.5%
420
85.0%
27
5.5%
22
4.5%
5
1.0%
Total
115
4699
100.0%
363
7.7%
4121
87.7%
297
6.3%
170
3.6%
45
1.0%
GB
Parallel
247
23157
96.6%
1345
5.8%
20647
89.2%
1812
7.8%
1115
4.8%
50
0.2%
Serial/Extended
27
819
3.4%
72
8.8%
711
86.8%
48
5.9%
30
3.7%
6
0.7%
Total
274
23976
100.0%
1417
5.9%
21358
89.1%
1860
7.8%
1145
4.8%
56
0.2%
157.00
115.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2018)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
96.2%
7274
4.7% 137402
89.3%
12353
8.0%
8482
5.5%
775
0.5%
Serial/Extended
49
6075
3.8%
444
7.3%
5378
88.5%
403
6.6%
241
4.0%
12
0.2%
Total
1188 160008
100.0%
7718
4.8% 142780
89.2%
12756
8.0%
8723
5.5%
787
0.5%
Scotland
Parallel
30
2231
99.6%
180
8.1%
1913
85.7%
200
9.0%
138
6.2%
0
0.0 %
Serial/Extended
1
10
0.4%
1
0.0%
8
80.0%
1
10.0%
1
10.0%
0
0.0 %
Total
31
2241
100.0%
181
8.1%
1921
85.7%
201
9.0%
139
6.2%
0
0.0 %
Wales
Parallel
872
43098
95.5%
1777
4.1%
37708
87.5%
3512
8.1%
2549
5.9%
1064
2.5%
Serial/Extended
68
2015
4.5%
173
8.6%
1608
79.8%
213
10.6%
162
8.0%
72
3.6%
Total
940
45113
100.0%
1950
4.3%
39316
87.2%
3725
8.3%
2711
6.0%
1136
2.5%
GB
Parallel
xx-xxxx-xxxx
96.1%
9231
4.6% 177023
88.8%
16065
8.1%
11169
5.6%
1839
0.9%
Serial/Extended
118
8100
3.9%
618
7.6%
6994
86.3%
617
7.6%
404
5.0%
84
1.0%
Total
xx-xxxx-xxxx
100.0%
9849
4.7% 184017
88.7%
16682
8.0%
11573
5.6%
1923
0.9%
#######
940.00
31.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
26
809
3.4%
72
8.9%
701
86.7%
48
5.9 %
30
3.7 %
6
0.7 %
Parallel - Herd or Group Slaughter
7
423
1.8%
20
4.7%
392
92.7%
19
4.5 %
11
2.6 %
0
Parallel New Infection
109
11297
47.1%
560
5.0%
10112
89.5%
1016
9.0 %
619
5.5 %
6
0.1 %
Parallel - new infection cull area
37
4511
18.8%
267
5.9%
4007
88.8%
340
7.5 %
230
5.1 %
7
0.2 %
Parallel - Other
9
1235
5.2%
78
6.3%
1108
89.7%
66
5.3 %
48
3.9 %
1
0.1 %
Parallel Persistent Infection
42
4932
20.6%
366
7.4%
4384
88.9%
321
6.5 %
175
3.5 %
7
0.1 %
Parallel - persistent infection cull area
5
596
2.5%
19
3.2%
551
92.4%
41
6.9 %
26
4.4 %
0
Parallel - Rapid Testing Two-times IRs
38
163
0.7%
35
21.5%
93
57.1%
9
5.5 %
6
3.7 %
29
17.8 %
Serial - Suspected NSR Herd
1
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Total
274
23976
100.0%
1417
5.9%
21358
89.1%
1860
7.8 %
1145
4.8 %
56
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (October 2018)
England - HRA
Extended Flexible IFN-GAMMA test
5
315
1.3%
25
7.9%
281
89.2%
21
6.7 %
8
2.5 %
1
0.3 %
Parallel New Infection
3
891
3.7%
62
7.0%
805
90.3%
44
4.9 %
24
2.7 %
0
Parallel - new infection cull area
37
4511
18.8%
267
5.9%
4007
88.8%
340
7.5 %
230
5.1 %
7
0.2 %
Parallel - Other
7
1215
5.1%
71
5.8%
1095
90.1%
66
5.4 %
48
4.0 %
1
0.1 %
Parallel Persistent Infection
13
2421
10.1%
139
5.7%
2209
91.2%
146
6.0 %
73
3.0 %
0
Parallel - persistent infection cull area
5
596
2.5%
19
3.2%
551
92.4%
41
6.9 %
26
4.4 %
0
England - HRA
70
9949
41.5%
583
5.9%
8948
89.9%
658
6.6 %
409
4.1 %
9
0.1 %
England - Edge
Parallel New Infection
78
8216
34.3%
429
5.2%
7267
88.4%
822
10.0 %
518
6.3 %
2
0.0 %
Parallel Persistent Infection
1
11
0.0%
0
0.0 %
8
72.7%
3
27.3 %
3
27.3 %
0
Serial - Suspected NSR Herd
1
10
0.0%
0
0.0 %
10
100.0%
0
0
0
England - Edge
80
8237
34.4%
429
5.2%
7285
88.4%
825
10.0 %
521
6.3 %
2
0.0 %
England - LRA
Parallel New Infection
7
1076
4.5%
42
3.9%
990
92.0%
79
7.3 %
44
4.1 %
0
England - LRA
7
1076
4.5%
42
3.9%
990
92.0%
79
7.3 %
44
4.1 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
16
410
1.7%
40
9.8%
348
84.9%
21
5.1 %
17
4.1 %
5
1.2 %
Parallel - Herd or Group Slaughter
6
369
1.5%
20
5.4%
341
92.4%
13
3.5 %
8
2.2 %
0
Parallel New Infection
4
163
0.7%
11
6.7%
143
87.7%
12
7.4 %
7
4.3 %
2
1.2 %
Parallel - Other
1
9
0.0%
2
22.2%
7
77.8%
0
0
0
Parallel Persistent Infection
27
2399
10.0%
224
9.3%
2076
86.5%
164
6.8 %
92
3.8 %
7
0.3 %
Parallel - Rapid Testing Two-times IRs
34
153
0.6%
33
21.6%
85
55.6%
9
5.9 %
6
3.9 %
29
19.0 %
Wales - High TB
88
3503
14.6%
330
9.4%
3000
85.6%
219
6.3 %
130
3.7 %
43
1.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
84
0.4%
7
8.3%
72
85.7%
6
7.1 %
5
6.0 %
0
Parallel - Herd or Group Slaughter
1
54
0.2%
0
0.0 %
51
94.4%
6
11.1 %
3
5.6 %
0
Parallel New Infection
12
582
2.4%
12
2.1%
553
95.0%
39
6.7 %
17
2.9 %
0
Parallel - Other
1
11
0.0%
5
45.5%
6
54.5%
0
0
0
Parallel Persistent Infection
1
101
0.4%
3
3.0%
91
90.1%
8
7.9 %
7
6.9 %
0
Parallel - Rapid Testing Two-times IRs
3
9
0.0%
2
22.2%
7
77.8%
0
0
0
Wales - Intermediate TB
23
841
3.5%
29
3.4%
780
92.7%
59
7.0 %
32
3.8 %
0
Wales - Low TB
Parallel New Infection
3
354
1.5%
4
1.1%
340
96.0%
19
5.4 %
8
2.3 %
2
0.6 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
4
355
1.5%
4
1.1%
341
96.1%
19
5.4 %
8
2.3 %
2
0.6 %
Scotland - Scotland
Parallel New Infection
2
15
0.1%
0
0.0 %
14
93.3%
1
6.7 %
1
6.7 %
0
Scotland - Scotland
2
15
0.1%
0
0.0 %
14
93.3%
1
6.7 %
1
6.7 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
104
7801
3.8%
560
7.2%
6761
86.7%
604
7.7 %
396
5.1 %
84
1.1 %
Parallel - Herd or Group Slaughter
89
5696
2.7%
224
3.9%
4711
82.7%
544
9.6 %
391
6.9 %
370
6.5 %
Parallel New Infection
939 109509
52.8%
4118
3.8%
98646
90.1%
9145
8.4 %
6285
5.7 %
460
0.4 %
Parallel - new infection cull area
204
24638
11.9%
1329
5.4%
21775
88.4%
2040
8.3 %
1367
5.5 %
167
0.7 %
Parallel - Other
51
2585
1.2%
225
8.7%
2143
82.9%
147
5.7 %
108
4.2 %
109
4.2 %
Parallel Persistent Infection
388
49364
23.8%
2717
5.5%
43380
87.9%
3687
7.5 %
2665
5.4 %
602
1.2 %
Parallel - persistent infection cull area
32
6155
3.0%
339
5.5%
5428
88.2%
419
6.8 %
288
4.7 %
100
1.6 %
Parallel - Rapid Testing Two-times IRs
338
1315
0.6%
279
21.2%
940
71.5%
83
6.3 %
65
4.9 %
31
2.4 %
Serial - Anomalous Reaction
10
270
0.1%
58
21.5%
204
75.6%
13
4.8 %
8
3.0 %
0
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
9849
4.7%
184017
88.7%
16682
8.0 %
11573
5.6 %
1923
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2018)
England - HRA
Extended Flexible IFN-GAMMA test
37
5876
2.8%
441
7.5%
5190
88.3%
391
6.7 %
233
4.0 %
12
0.2 %
Parallel New Infection
19
2678
1.3%
157
5.9%
2346
87.6%
245
9.1 %
163
6.1 %
12
0.4 %
Parallel - new infection cull area
204
24638
11.9%
1329
5.4%
21775
88.4%
2040
8.3 %
1367
5.5 %
167
0.7 %
Parallel - Other
27
2315
1.1%
161
7.0%
1943
83.9%
138
6.0 %
102
4.4 %
109
4.7 %
Parallel Persistent Infection
133
26852
12.9%
1572
5.9%
23933
89.1%
1843
6.9 %
1291
4.8 %
56
0.2 %
Parallel - persistent infection cull area
32
6155
3.0%
339
5.5%
5428
88.2%
419
6.8 %
288
4.7 %
100
1.6 %
Serial - Anomalous Reaction
8
170
0.1%
3
1.8%
159
93.5%
12
7.1 %
8
4.7 %
0
England - Edge
Parallel New Infection
611
77650
37.4%
3164
4.1%
69852
90.0%
6520
8.4 %
4408
5.7 %
226
0.3 %
Parallel - Other
2
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel Persistent Infection
27
4008
1.9%
210
5.2%
3408
85.0%
334
8.3 %
288
7.2 %
102
2.5 %
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
England - LRA
Parallel New Infection
81
9008
4.3%
325
3.6%
8135
90.3%
780
8.7 %
545
6.1 %
3
0.0 %
Parallel Persistent Infection
3
627
0.3%
17
2.7%
581
92.7%
33
5.3 %
29
4.6 %
0
England
1188 160008
77.2%
7718
4.8%
142780
89.2%
12756
8.0 %
8723
5.5 %
787
0.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
54
1665
0.8%
108
6.5%
1336
80.2%
196
11.8 %
149
8.9 %
72
4.3 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2018) Cont.
Parallel - Herd or Group Slaughter
79
4787
2.3%
218
4.6%
3976
83.1%
324
6.8 %
224
4.7 %
369
7.7 %
Parallel New Infection
24
869
0.4%
21
2.4%
810
93.2%
52
6.0 %
36
4.1 %
2
0.2 %
Parallel - Other
19
209
0.1%
19
9.1%
185
88.5%
7
3.3 %
5
2.4 %
0
Parallel Persistent Infection
217
17504
8.4%
890
5.1%
15162
86.6%
1412
8.1 %
1008
5.8 %
444
2.5 %
Parallel - Rapid Testing Two-times IRs
302
1175
0.6%
248
21.1%
835
71.1%
79
6.7 %
62
5.3 %
30
2.6 %
Serial - Anomalous Reaction
2
100
0.0%
55
55.0%
45
45.0%
1
1.0 %
0
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
10
204
0.1%
10
4.9%
182
89.2%
14
6.9 %
12
5.9 %
0
Parallel - Herd or Group Slaughter
10
909
0.4%
6
0.7%
735
80.9%
220
24.2 %
167
18.4 %
1
0.1 %
Parallel New Infection
126
11821
5.7%
210
1.8%
10881
92.0%
916
7.7 %
672
5.7 %
58
0.5 %
Parallel - Other
1
11
0.0%
5
45.5%
6
54.5%
0
0
0
Parallel Persistent Infection
7
368
0.2%
28
7.6%
294
79.9%
62
16.8 %
46
12.5 %
0
Parallel - Rapid Testing Two-times IRs
32
125
0.1%
29
23.2%
92
73.6%
4
3.2 %
3
2.4 %
1
0.8 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
46
0.0%
0
0.0 %
45
97.8%
2
4.3 %
1
2.2 %
0
Parallel New Infection
49
5298
2.6%
100
1.9%
4716
89.0%
433
8.2 %
323
6.1 %
159
3.0 %
Parallel - Other
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
2
40.0%
3
60.0 %
3
60.0 %
0
Parallel - Rapid Testing Two-times IRs
4
15
0.0%
2
13.3%
13
86.7%
0
0
0
Wales
940
45113
21.8%
1950
4.3%
39316
87.2%
3725
8.3 %
2711
6.0 %
1136
2.5 %
Scotland - Scotland
Extended Flexible IFN-GAMMA test
1
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Parallel New Infection
29
2185
1.1%
141
6.5%
1906
87.2%
199
9.1 %
138
6.3 %
0
Parallel - Other
1
46
0.0%
39
84.8%
7
15.2%
1
2.2 %
0
0
Scotland
31
2241
1.1%
181
8.1%
1921
85.7%
201
9.0 %
139
6.2 %
0

Table 5a. Summary of PM and culture results for IFNg positive animals.
Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2017 November
157
10666
108
961
2
24
0
0
0
0
87
3
0
8
0
800
0
31
1
0
December
114
9478
81
668
43
0
0
0
0
29
2
0
1
0
562
0
28
1
1
2018 January
207
19678
140
969
5
3
0
0
0
0
80
3
0
5
8
756
0
84
0
24
February
206
20791
130
1229
2
22
0
0
0
0
72
7
1
8
0
1075
1
35
0
5
March
189
17292
131
1199
1
3
0
0
0
0
121
6
0
4
0
987
0
72
1
2
April
195
20517
118
953
4
16
0
0
0
0
116
1
0
0
0
748
0
66
1
1
May
250
21066
131
947
1
2
0
0
0
0
121
1
0
4
0
781
0
35
1
1
June
187
18891
110
701
2
2
0
0
0
0
78
2
0
12
0
579
0
25
0
0
July
207
24111
119
806
1
12
0
0
0
0
67
0
3
2
1
675
3
36
0
4
August
228
19599
120
682
23
30
0
0
0
0
59
0
0
4
4
523
7
23
0
1
September
216
21441
137
946
6
15
0
0
0
0
33
0
3
0
0
833
56
0
0
0
October
274
23976
152
1417
479
464
0
0
0
0
19
0
0
0
0
195
5
0
0
0
Totals (last
12 months)
2430
227506
1477
11478
526
636
0
0
0
0
882
25
7
48
13
8514
72
435
5
39

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)
Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1303
175903
911
8828
478
0
0
0
0
752
13
5
29
2
6941
5
38
1
2
Scotland
37
3082
25
352
47
0
0
0
0
33
4
0
0
8
188
0
37
1
27
Wales
1090
48521
541
2298
111
0
0
0
0
97
8
2
19
3
1385
67
360
3
10
Totals
2430
227506
1477
11478
636
0
0
0
0
882
25
7
48
13
8514
72
435
5
39

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
40
106
53
87
48
87
50
86
47
85
65
95
45
72
69
75
39
52
37
53
45
83
39
68
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2016
2017
2018
IFNg Positive herds
IFNg Negative herds
7789
542
6091
549
7323
420
9568
453
9409
536
8947
398
10544
376
8010
424
7065
288
6507
334
8559
647
7448
325
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22358
1416
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2016
2017
2018
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2016
2017
2018
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
W Sussex
W Glamorgan
Somerset
Powys
Oxon
Northants
Leics & Rut
Heref
Gwent
E Sussex
Devon
Cumbria
Cheshire
Carms
Berks
Aberdeenshire
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during October 2018 (% submission per
County)
County
%Resampled %Retested
Aberdeenshire
6.67 %
6.67 %
Avon
50.00 %
50.00 %
Berks
1.96 %
1.96 %
Bucks
8.33 %
12.50 %
Carms
4.05 %
5.86 %
Ceredigion
6.49 %
10.39 %
Cheshire
8.38 %
11.98 %
Corn & Scilly
6.53 %
8.73 %
Cumbria
5.38 %
8.80 %
Derbyshire
5.56 %
9.92 %
Devon
3.61 %
6.56 %
Dorset
5.55 %
7.27 %
E Sussex
5.95 %
8.33 %
Gloucs
3.97 %
8.22 %
Gwent
4.17 %
19.91 %
Hampshire
Heref
7.11 %
8.63 %
Kent
2.62 %
5.70 %
Leics & Rut
2.40 %
5.82 %
NE Wales
3.11 %
6.22 %
Northants
4.17 %
8.33 %
Notts
10.17 %
11.86 %
Oxon
5.73 %
8.82 %
Pembs
3.07 %
4.07 %
Powys
3.98 %
6.39 %
Shropshire
3.31 %
4.56 %
Somerset
3.62 %
4.71 %
Warks
2.19 %
3.95 %
W Glamorgan
Wiltshire
2.80 %
4.56 %
W Sussex
27.78 %
33.33 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19127
1054
5.5%
19262
1054
5.5%
Wales
4633
362
7.8%
4699
363
7.7%
Scotland
14
0
0.0 %
15
0.0 %
GB
23774
1416
6.0%
23976
1417
5.9%
Table 6a (i): Number of animals and submissions tested in October 2018
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
140798
7718
5.5%
160008
7718
4.8%
Wales
39469
1949
4.9%
45113
1950
4.3%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
182330
9848
5.4%
207362
9849
4.7%
Table 6b (i): Number of animals and submissions tested in 2018 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
12152
844
6.9%
12264
844
6.9%
Wales
2265
120
5.3%
2301
120
5.2%
Scotland
189
7
3.7%
189
7
3.7%
GB
14606
971
6.6%
14754
971
6.6%
Table 6a (ii): Number of animals and submissions tested in October 2017
Table 6b (ii): Number of animals and submissions tested in 2017 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
65172
3984
6.1%
82634
3984
4.8%
Wales
29318
1966
6.7%
32252
1966
6.1%
Scotland
2368
57
2.4%
2426
57
2.3%
GB
96855
6007
6.2%
117312
6007
5.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Berkshire
PRV-SP
1
2
0
Cheshire
PRV-SP
1
1
1
100.00 %
Cornwall and Isles of Scilly
PRV-SP
1
1
0
Devon
PRV-SP
1
2
0
Dorset
PRV-SE
1
2
0
PRV-SP
1
1
0
Hampshire
PRV-SE
1
1
0
Total
7
10
1
10.00 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples October 2018
Table 7b Number of private samples 2018
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
2
0
Buckinghamshire
PRV-SE
1
1
0
Cheshire
PRV-SP
2
2
1
50.00 %
Cornwall and Isles of Scilly
PRV-SE
1
3
0
PRV-SP
1
1
0
Devon
PRV-SE
1
2
1
50.00 %
PRV-SP
1
2
0
Dorset
PRV-SE
4
25
6
24.00 %
PRV-SP
1
1
0
East Sussex
PRV-SE
1
1
0
PRV-SP
1
1
0
Hampshire
PRV-SE
2
6
1
16.67 %
Northern Somerset & South Glou
PRV-SE
2
46
8
17.39 %
Oxfordshire
PRV-SE
1
38
4
10.53 %
Somerset excl North
PRV-SE
3
46
3
6.52 %
PRV-SP
1
1
0
Suffolk
PRV-SP
2
11
0
Total
26
189
24
12.70 %
Isle of Man
SER-IOM
16
49
1
2.04 %
Isle of Man
SER-IOM
2
4
1
25.00 %

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
South Wales
1
65
15
23.1 %
TC0077
Sum:
65
15
23.1 %
Table 8a Number of Cattle Antibody Tests October 2018
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Argyll and Bute
1
3
3
100.0 %
Carmarthenshire
2
170
4
2.4 %
Ceredigion
1
85
4
4.7 %
Pembrokeshire
3
201
10
5.0 %
Powys
1
345
3
0.9 %
South Wales
5
199
17
8.5 %
TC0077
Sum:
1,003
41
4.1 %
Table 8b Number of Cattle Antibody Tests 2018