Monthly report: Surveillance Project SB4008 IFNg tests for Bovine Tuberculosis - February 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 150
Report Period 1st - 28th February 2019
Issued 14 March 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
February
2019
%
Total 2019
%
Submissions
320
609
Samples
Total (%)
28044
100.00 %
55557
100.00 %
England
22715
81.00 %
43300
77.94 %
Wales
5329
19.00 %
12257
22.06 %
Scotland
Parallel Tests
PAR-CUL-N
4898
17.47 %
7813
14.06 %
PAR-CUL-P
3318
11.83 %
5463
9.83 %
PAR-HERD-S
1042
3.72 %
1676
3.02 %
PAR-NEW-IN
13851
49.39 %
27807
50.05 %
PAR-OTHER
304
1.08 %
1909
3.44 %
PAR-PERSIS
3805
13.57 %
9433
xx-xxxx-xxxx %
PAR-RAPID
120
0.43 %
293
0.53 %
Total(% of all samples)
27338
97.48 %
54394
97.91 %
Serial/Extended Tests
SER-FLEXI
697
2.49 %
1147
2.06 %
SER-FRAUD
9
0.03 %
9
0.02 %
SER-NSR
0
0.00 %
7
0.01 %
SER-OTHER
0
0.00 %
Total(% of all samples)
706
2.52 %
1163
2.09 %
Total
28044
100.00 %
55557
100.00 %
Retests
Total (% of all samples)
2301
8.20 %
4238
7.63 %
England
1798
6.41 %
3324
5.98 %
Wales
503
1.79 %
914
1.65 %
Scotland
Resamples
Total (% of all samples)
1908
6.80 %
3565
6.42 %
England
1479
5.27 %
2814
5.07 %
Wales
429
1.53 %
751
1.35 %
Scotland
Rejects
Total (% of all samples)
221
0.79 %
1195
2.15 %
England
217
0.77 %
1077
1.94 %
Wales
4
0.01 %
118
0.21 %
Scotland
0
0.00 %
Table 1. Monthly Totals for February 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-FRAUD
Breakdown of
Reason for
submission
PAR-CUL-N
4898
17.5 %
PAR-CUL-P
3318
11.8 %
PAR-HERD-S
1042
3.7 %
PAR-NEW-IN
13851
49.4 %
PAR-OTHER
304
1.1 %
PAR-PERSIS
3805
13.6 %
PAR-RAPID
120
0.4 %
SER-FLEXI
697
2.5 %
SER-FRAUD
9
0.0 %
Total
28044
100.00 %
Figure 1. Number of samples submitted in each category during February 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
W Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during February 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
1000
2000
3000
4000
5000
6000
7000
8000
9000
W Glamorgan
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
S Yorks
Staffs
Somerset
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
19
0.07 %
1
5.26 %
14
73.68 %
4
21.05 %
4
21.05 %
0
0.00 %
SER-FRAUD
1
9
0.03 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
6
402
1.43 %
19
4.73 %
288
71.64 %
43
10.70 %
45
11.19 %
50
12.44 %
PAR-CUL-P
2
176
0.63 %
2
1.14 %
162
92.05 %
13
7.39 %
12
6.82 %
0
0.00 %
Devon
PAR-CUL-N
7
1,146
4.09 %
83
7.24 %
988
86.21 %
87
7.59 %
72
6.28 %
3
0.26 %
PAR-CUL-P
9
2,667
9.51 %
200
7.50 %
2307
86.50 %
191
7.16 %
157
5.89 %
3
0.11 %
PAR-NEW-IN
1
131
0.47 %
7
5.34 %
110
83.97 %
13
9.92 %
14
10.69 %
0
0.00 %
PAR-OTHER
2
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-PERSIS
4
619
2.21 %
63
10.18 %
524
84.65 %
40
6.46 %
31
5.01 %
1
0.16 %
SER-FLEXI
2
250
0.89 %
30
12.00 %
200
80.00 %
22
8.80 %
6
2.40 %
14
5.60 %
Dorset
PAR-CUL-N
8
2,083
7.43 %
162
7.78 %
1823
87.52 %
117
5.62 %
95
4.56 %
3
0.14 %
PAR-CUL-P
1
108
0.39 %
7
6.48 %
94
87.04 %
9
8.33 %
7
6.48 %
0
0.00 %
Gloucs
PAR-CUL-N
8
907
3.23 %
47
5.18 %
788
86.88 %
71
7.83 %
65
7.17 %
7
0.77 %
Heref
PAR-CUL-N
3
229
0.82 %
5
2.18 %
185
80.79 %
35
15.28 %
28
12.23 %
11
4.80 %
PAR-PERSIS
1
22
0.08 %
2
9.09 %
10
45.45 %
10
45.45 %
10
45.45 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
71
0.25 %
14
19.72 %
56
78.87 %
1
1.41 %
0
0.00 %
1
1.41 %
PAR-PERSIS
3
68
0.24 %
4
5.88 %
53
77.94 %
12
17.65 %
11
16.18 %
0
0.00 %
Somerset
PAR-CUL-N
2
131
0.47 %
6
4.58 %
114
87.02 %
11
8.40 %
11
8.40 %
0
0.00 %
Staffs
PAR-PERSIS
1
136
0.48 %
109
80.15 %
19
13.97 %
8
5.88 %
8
5.88 %
0
0.00 %
Wiltshire
PAR-CUL-P
1
367
1.31 %
69
18.80 %
290
79.02 %
12
3.27 %
7
1.91 %
1
0.27 %
PAR-PERSIS
1
257
0.92 %
3
1.17 %
240
93.39 %
11
4.28 %
9
3.50 %
5
1.95 %
England - Edge
Berks
PAR-NEW-IN
7
799
2.85 %
42
5.26 %
692
86.61 %
79
9.89 %
65
8.14 %
0
0.00 %
Bucks
PAR-NEW-IN
3
23
0.08 %
1
4.35 %
16
69.57 %
6
26.09 %
6
26.09 %
0
0.00 %
Cheshire
PAR-NEW-IN
26
3,809
13.58 %
117
3.07 %
3515
92.28 %
231
6.06 %
177
4.65 %
0
0.00 %
Derbyshire
PAR-NEW-IN
23
1,331
4.75 %
85
6.39 %
1056
79.34 %
211
15.85 %
190
14.27 %
0
0.00 %
Table 2a. Summary by County for February 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
E Sussex
PAR-NEW-IN
3
369
1.32 %
23
6.23 %
334
90.51 %
16
4.34 %
12
3.25 %
0
0.00 %
Hampshire
PAR-NEW-IN
3
609
2.17 %
29
4.76 %
541
88.83 %
43
7.06 %
39
6.40 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
8
774
2.76 %
65
8.40 %
635
82.04 %
70
9.04 %
66
8.53 %
8
1.03 %
Northants
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Notts
PAR-NEW-IN
1
89
0.32 %
4
4.49 %
84
94.38 %
2
2.25 %
0
0.00 %
1
1.12 %
Oxon
PAR-NEW-IN
22
2,358
8.41 %
122
5.17 %
2032
86.17 %
202
8.57 %
154
6.53 %
50
2.12 %
Warks
PAR-NEW-IN
18
2,012
7.17 %
124
6.16 %
1731
86.03 %
176
8.75 %
134
6.66 %
23
1.14 %
England - LRA
Cambs
PAR-NEW-IN
2
165
0.59 %
3
1.82 %
151
91.52 %
4
2.42 %
3
1.82 %
8
4.85 %
Lincs
PAR-NEW-IN
1
10
0.04 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
3
560
2.00 %
19
3.39 %
476
85.00 %
44
7.86 %
37
6.61 %
28
5.00 %
England
188
22715
81.00 %
1471
6.48 %
19548
86.06 %
1798
7.92 %
1479
6.51 %
217
0.96 %
Wales - High TB
Carms
PAR-HERD-S
9
566
2.02 %
29
5.12 %
485
85.69 %
59
10.42 %
51
9.01 %
1
0.18 %
PAR-PERSIS
11
707
2.52 %
49
6.93 %
604
85.43 %
61
8.63 %
54
7.64 %
0
0.00 %
PAR-RAPID
7
27
0.10 %
9
33.33 %
17
62.96 %
1
3.70 %
1
3.70 %
0
0.00 %
SER-FLEXI
8
193
0.69 %
21
10.88 %
157
81.35 %
20
10.36 %
15
7.77 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
11
0.04 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-PERSIS
4
518
1.85 %
77
14.86 %
387
74.71 %
62
11.97 %
54
10.42 %
0
0.00 %
PAR-RAPID
6
19
0.07 %
7
36.84 %
10
52.63 %
2
10.53 %
2
10.53 %
0
0.00 %
Gwent
PAR-HERD-S
3
447
1.59 %
31
6.94 %
386
86.35 %
28
6.26 %
28
6.26 %
2
0.45 %
PAR-PERSIS
4
66
0.24 %
3
4.55 %
58
87.88 %
7
10.61 %
5
7.58 %
0
0.00 %
PAR-RAPID
3
7
0.02 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
3
5
0.02 %
2
40.00 %
2
40.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-OTHER
3
173
0.62 %
10
5.78 %
147
84.97 %
24
13.87 %
16
9.25 %
0
0.00 %
PAR-PERSIS
9
1,390
4.96 %
93
6.69 %
1184
85.18 %
134
9.64 %
112
8.06 %
1
0.07 %
PAR-RAPID
9
25
0.09 %
9
36.00 %
13
52.00 %
3
12.00 %
3
12.00 %
0
0.00 %
SER-FLEXI
3
86
0.31 %
5
5.81 %
72
83.72 %
9
10.47 %
9
10.47 %
0
0.00 %
Powys
PAR-HERD-S
2
8
0.03 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
39
0.14 %
5
12.82 %
30
76.92 %
4
10.26 %
4
10.26 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for February 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
3
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
10
21
0.07 %
5
23.81 %
12
57.14 %
4
19.05 %
4
19.05 %
0
0.00 %
W Glamorgan
PAR-RAPID
1
4
0.01 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Wales - Intermediate TB
NE Wales
PAR-NEW-IN
12
702
2.50 %
34
4.84 %
622
88.60 %
54
7.69 %
46
6.55 %
0
0.00 %
PAR-OTHER
2
54
0.19 %
16
29.63 %
37
68.52 %
3
5.56 %
1
1.85 %
0
0.00 %
PAR-RAPID
4
17
0.06 %
4
23.53 %
12
70.59 %
1
5.88 %
1
5.88 %
0
0.00 %
SER-FLEXI
6
168
0.60 %
15
8.93 %
139
82.74 %
18
10.71 %
14
8.33 %
0
0.00 %
Powys
PAR-NEW-IN
1
10
0.04 %
0
0.00 %
9
90.00 %
1
10.00 %
1
10.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
1
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
3
57
0.20 %
4
7.02 %
49
85.96 %
4
7.02 %
4
7.02 %
0
0.00 %
Wales
132
5329
19.00 %
436
8.18 %
4460
83.69 %
503
9.44 %
429
8.05 %
4
0.08 %
Grand Total
Sum:
320
28044
1907
24008
2301
1908
221
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
3
219
0.39 %
13
5.94 %
183
83.56 %
24
10.96 %
21
9.59 %
2
0.91 %
SER-FRAUD
1
9
0.02 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
13
1,099
1.98 %
46
4.19 %
926
84.26 %
84
7.64 %
77
7.01 %
50
4.55 %
PAR-CUL-P
4
1,165
2.10 %
19
1.63 %
1035
88.84 %
132
11.33 %
109
9.36 %
2
0.17 %
PAR-PERSIS
2
106
0.19 %
1
0.94 %
9
8.49 %
1
0.94 %
1
0.94 %
95
89.62 %
SER-FLEXI
2
15
0.03 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
14
1,795
3.23 %
135
7.52 %
1539
85.74 %
133
7.41 %
117
6.52 %
4
0.22 %
PAR-CUL-P
10
2,954
5.32 %
208
7.04 %
2564
86.80 %
217
7.35 %
179
6.06 %
3
0.10 %
PAR-NEW-IN
2
475
0.85 %
74
15.58 %
352
74.11 %
55
11.58 %
49
10.32 %
0
0.00 %
PAR-OTHER
2
5
0.01 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-PERSIS
9
1,330
2.39 %
111
8.35 %
1141
85.79 %
98
7.37 %
77
5.79 %
1
0.08 %
SER-FLEXI
4
312
0.56 %
36
11.54 %
225
72.12 %
22
7.05 %
6
1.92 %
45
14.42 %
Dorset
PAR-CUL-N
13
2,461
4.43 %
185
7.52 %
2137
86.83 %
158
6.42 %
135
5.49 %
4
0.16 %
PAR-CUL-P
3
977
1.76 %
45
4.61 %
889
90.99 %
46
4.71 %
43
4.40 %
0
0.00 %
Gloucs
PAR-CUL-N
14
1,527
2.75 %
77
5.04 %
1234
80.81 %
124
8.12 %
109
7.14 %
107
7.01 %
Heref
PAR-CUL-N
5
572
1.03 %
20
3.50 %
496
86.71 %
53
9.27 %
45
7.87 %
11
1.92 %
PAR-PERSIS
1
22
0.04 %
2
9.09 %
10
45.45 %
10
45.45 %
10
45.45 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
525
0.94 %
38
7.24 %
448
85.33 %
45
8.57 %
38
7.24 %
1
0.19 %
PAR-PERSIS
4
140
0.25 %
6
4.29 %
109
77.86 %
27
19.29 %
25
17.86 %
0
0.00 %
Somerset
PAR-CUL-N
3
359
0.65 %
27
7.52 %
310
86.35 %
28
7.80 %
20
5.57 %
2
0.56 %
PAR-OTHER
2
238
0.43 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
Staffs
PAR-PERSIS
1
136
0.24 %
109
80.15 %
19
13.97 %
8
5.88 %
8
5.88 %
0
0.00 %
Wiltshire
PAR-CUL-P
1
367
0.66 %
69
18.80 %
290
79.02 %
12
3.27 %
7
1.91 %
1
0.27 %
PAR-OTHER
1
36
0.06 %
1
2.78 %
33
91.67 %
4
11.11 %
2
5.56 %
0
0.00 %
PAR-PERSIS
1
257
0.46 %
3
1.17 %
240
93.39 %
11
4.28 %
9
3.50 %
5
1.95 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
14
1,808
3.25 %
65
3.60 %
1613
89.21 %
151
8.35 %
130
7.19 %
0
0.00 %
PAR-PERSIS
1
92
0.17 %
4
4.35 %
87
94.57 %
2
2.17 %
1
1.09 %
0
0.00 %
Bucks
PAR-NEW-IN
4
176
0.32 %
13
7.39 %
154
87.50 %
13
7.39 %
9
5.11 %
0
0.00 %
Cheshire
PAR-NEW-IN
49
8,488
15.28 %
225
2.65 %
7687
90.56 %
525
6.19 %
426
5.02 %
150
1.77 %
PAR-PERSIS
1
13
0.02 %
1
7.69 %
12
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
41
4,128
7.43 %
170
4.12 %
3421
82.87 %
365
8.84 %
324
7.85 %
213
5.16 %
E Sussex
PAR-NEW-IN
8
1,176
2.12 %
69
5.87 %
1053
89.54 %
46
3.91 %
41
3.49 %
13
1.11 %
Hampshire
PAR-NEW-IN
9
664
1.20 %
30
4.52 %
592
89.16 %
46
6.93 %
42
6.33 %
0
0.00 %
SER-NSR
1
7
0.01 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
13
1,249
2.25 %
87
6.97 %
951
76.14 %
124
9.93 %
116
9.29 %
95
7.61 %
Northants
PAR-NEW-IN
2
9
0.02 %
0
0.00 %
8
88.89 %
2
22.22 %
1
11.11 %
0
0.00 %
Notts
PAR-NEW-IN
4
466
0.84 %
19
4.08 %
343
73.61 %
46
9.87 %
40
8.58 %
64
13.73 %
Oxon
PAR-NEW-IN
39
4,216
7.59 %
223
5.29 %
3602
85.44 %
400
9.49 %
341
8.09 %
50
1.19 %
Warks
PAR-NEW-IN
28
2,558
4.60 %
148
5.79 %
2159
84.40 %
214
8.37 %
169
6.61 %
82
3.21 %
PAR-PERSIS
2
157
0.28 %
9
5.73 %
137
87.26 %
12
7.64 %
11
7.01 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
3
172
0.31 %
3
1.74 %
156
90.70 %
6
3.49 %
5
2.91 %
8
4.65 %
Cumbria
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
22
0.04 %
0
0.00 %
21
95.45 %
1
4.55 %
1
4.55 %
0
0.00 %
Lincs
PAR-NEW-IN
4
70
0.13 %
11
15.71 %
45
64.29 %
14
20.00 %
14
20.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
3
560
1.01 %
19
3.39 %
476
85.00 %
44
7.86 %
37
6.61 %
28
5.00 %
S Yorks
PAR-NEW-IN
1
162
0.29 %
4
2.47 %
150
92.59 %
10
6.17 %
8
4.94 %
0
0.00 %
England
350
43300
77.94 %
2360
5.45 %
37049
85.56 %
3324
7.68 %
2814
6.50 %
1077
2.49 %
Wales - High TB
Carms
PAR-HERD-S
14
1,021
1.84 %
67
6.56 %
886
86.78 %
83
8.13 %
66
6.46 %
2
0.20 %
PAR-PERSIS
28
3,659
6.59 %
182
4.97 %
3306
90.35 %
200
5.47 %
158
4.32 %
13
0.36 %
PAR-RAPID
18
90
0.16 %
34
37.78 %
51
56.67 %
9
10.00 %
5
5.56 %
0
0.00 %
SER-FLEXI
12
298
0.54 %
27
9.06 %
250
83.89 %
26
8.72 %
21
7.05 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
11
0.02 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
8
828
1.49 %
94
11.35 %
666
80.43 %
79
9.54 %
68
8.21 %
0
0.00 %
PAR-RAPID
10
24
0.04 %
11
45.83 %
11
45.83 %
3
12.50 %
2
8.33 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-HERD-S
3
447
0.80 %
31
6.94 %
386
86.35 %
28
6.26 %
28
6.26 %
2
0.45 %
PAR-PERSIS
7
458
0.82 %
21
4.59 %
405
88.43 %
38
8.30 %
31
6.77 %
1
0.22 %
PAR-RAPID
11
43
0.08 %
13
30.23 %
26
60.47 %
4
9.30 %
3
6.98 %
1
2.33 %
Pembs
PAR-HERD-S
6
54
0.10 %
5
9.26 %
47
87.04 %
2
3.70 %
2
3.70 %
0
0.00 %
PAR-OTHER
8
1,030
1.85 %
40
3.88 %
914
88.74 %
94
9.13 %
76
7.38 %
0
0.00 %
PAR-PERSIS
15
1,787
3.22 %
111
6.21 %
1541
86.23 %
161
9.01 %
133
7.44 %
2
0.11 %
PAR-RAPID
22
58
0.10 %
16
27.59 %
38
65.52 %
5
8.62 %
4
6.90 %
0
0.00 %
SER-FLEXI
4
89
0.16 %
6
6.74 %
74
83.15 %
9
10.11 %
9
10.11 %
0
0.00 %
Powys
PAR-HERD-S
5
11
0.02 %
2
18.18 %
8
72.73 %
0
0.00 %
0
0.00 %
1
9.09 %
PAR-NEW-IN
5
46
0.08 %
5
10.87 %
35
76.09 %
6
13.04 %
6
13.04 %
0
0.00 %
PAR-PERSIS
5
216
0.39 %
32
14.81 %
174
80.56 %
14
6.48 %
10
4.63 %
0
0.00 %
PAR-RAPID
14
55
0.10 %
9
16.36 %
23
41.82 %
6
10.91 %
6
10.91 %
17
30.91 %
SER-FLEXI
1
14
0.03 %
1
7.14 %
13
92.86 %
0
0.00 %
0
0.00 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
117
0.21 %
7
5.98 %
108
92.31 %
5
4.27 %
2
1.71 %
0
0.00 %
PAR-PERSIS
1
7
0.01 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
1
4
0.01 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
20
0.04 %
2
10.00 %
12
60.00 %
6
30.00 %
6
30.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
19
1,217
2.19 %
52
4.27 %
1078
88.58 %
91
7.48 %
77
6.33 %
10
0.82 %
PAR-OTHER
2
54
0.10 %
16
29.63 %
37
68.52 %
3
5.56 %
1
1.85 %
0
0.00 %
PAR-PERSIS
1
6
0.01 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
5
19
0.03 %
4
21.05 %
14
73.68 %
1
5.26 %
1
5.26 %
0
0.00 %
SER-FLEXI
14
416
0.75 %
34
8.17 %
305
73.32 %
28
6.73 %
24
5.77 %
53
12.74 %
Powys
PAR-NEW-IN
1
10
0.02 %
0
0.00 %
9
90.00 %
1
10.00 %
1
10.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Glamorgan
PAR-HERD-S
2
11
0.02 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
5
85
0.15 %
4
4.71 %
75
88.24 %
5
5.88 %
5
5.88 %
1
1.18 %
NE Wales
PAR-NEW-IN
4
46
0.08 %
0
0.00 %
31
67.39 %
1
2.17 %
0
0.00 %
15
32.61 %
Wales
259
12257
22.06 %
830
6.77 %
10558
86.14 %
914
7.46 %
751
6.13 %
118
0.96 %
Grand Total
Sum:
609
55557
3190
47607
4238
3565
1195
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol February 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
185
22456
98.9%
1441
6.4%
19339
86.1%
1776
7.9%
1473
6.6%
203
0.9%
Serial/Extended
3
259
1.1%
30
11.6%
209
80.7%
22
8.5%
6
2.3%
14
5.4%
Total
188
22715
100.0%
1471
6.5%
19548
86.1%
1798
7.9%
1479
6.5%
217
1.0%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
115
4882
91.6%
395
8.1%
4092
83.8%
456
9.3%
391
8.0%
4
0.1%
Serial/Extended
17
447
8.4%
41
9.2%
368
82.3%
47
10.5%
38
8.5%
0
0.0 %
Total
132
5329
100.0%
436
8.2%
4460
83.7%
503
9.4%
429
8.1%
4
0.1%
GB
Parallel
300
27338
97.5%
1836
6.7%
23431
85.7%
2232
8.2%
1864
6.8%
207
0.8%
Serial/Extended
20
706
2.5%
71
10.1%
577
81.7%
69
9.8%
44
6.2%
14
2.0%
Total
320
28044
100.0%
1907
6.8%
24008
85.6%
2301
8.2%
1908
6.8%
221
0.8%
188.00
132.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
341
42955
99.2%
2324
5.4%
36792
85.7%
3301
7.7%
2807
6.5%
1032
2.4%
Serial/Extended
9
345
0.8%
36
10.4%
257
74.5%
23
6.7%
7
2.0%
45
13.0%
Total
350
43300
100.0%
2360
5.5%
37049
85.6%
3324
7.7%
2814
6.5%
1077
2.5%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
227
11439
93.3%
762
6.7%
9915
86.7%
851
7.4%
697
6.1%
65
0.6%
Serial/Extended
32
818
6.7%
68
8.3%
643
78.6%
63
7.7%
54
6.6%
53
6.5%
Total
259
12257
100.0%
830
6.8%
10558
86.1%
914
7.5%
751
6.1%
118
1.0%
GB
Parallel
568
54394
97.9%
3086
5.7%
46707
85.9%
4152
7.6%
3504
6.4%
1097
2.0%
Serial/Extended
41
1163
2.1%
104
8.9%
900
77.4%
86
7.4%
61
5.2%
98
8.4%
Total
609
55557
100.0%
3190
5.7%
47607
85.7%
4238
7.6%
3565
6.4%
1195
2.2%
350.00
259.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
19
697
2.5%
71
10.2%
568
81.5%
69
9.9 %
44
6.3 %
14
2.0 %
Parallel - Herd or Group Slaughter
19
1042
3.7%
66
6.3%
891
85.5%
90
8.6 %
82
7.9 %
3
0.3 %
Parallel New Infection
142
13851
49.4%
687
5.0%
12091
87.3%
1163
8.4 %
955
6.9 %
118
0.9 %
Parallel - new infection cull area
34
4898
17.5%
322
6.6%
4186
85.5%
364
7.4 %
316
6.5 %
74
1.5 %
Parallel - Other
10
304
1.1%
41
13.5%
244
80.3%
29
9.5 %
18
5.9 %
1
0.3 %
Parallel Persistent Infection
42
3805
13.6%
405
10.6%
3095
81.3%
349
9.2 %
298
7.8 %
7
0.2 %
Parallel - persistent infection cull area
13
3318
11.8%
278
8.4%
2853
86.0%
225
6.8 %
183
5.5 %
4
0.1 %
Parallel - Rapid Testing Two-times IRs
40
120
0.4%
37
30.8%
71
59.2%
12
10.0 %
12
10.0 %
0
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Total
320
28044
100.0%
1907
6.8%
24008
85.6%
2301
8.2 %
1908
6.8 %
221
0.8 %
Table 4a. Summaryof IFN Gamma results by submission reason (February 2019)
England - HRA
Extended Flexible IFN-GAMMA test
2
250
0.9%
30
12.0%
200
80.0%
22
8.8 %
6
2.4 %
14
5.6 %
Parallel New Infection
2
132
0.5%
7
5.3%
111
84.1%
13
9.8 %
14
10.6 %
0
Parallel - new infection cull area
34
4898
17.5%
322
6.6%
4186
85.5%
364
7.4 %
316
6.5 %
74
1.5 %
Parallel - Other
4
76
0.3%
15
19.7%
59
77.6%
2
2.6 %
1
1.3 %
1
1.3 %
Parallel Persistent Infection
11
1121
4.0%
182
16.2%
860
76.7%
85
7.6 %
73
6.5 %
6
0.5 %
Parallel - persistent infection cull area
13
3318
11.8%
278
8.4%
2853
86.0%
225
6.8 %
183
5.5 %
4
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - HRA
67
9804
35.0%
834
8.5%
8278
84.4%
711
7.3 %
593
6.0 %
99
1.0 %
England - Edge
Parallel New Infection
115
12176
43.4%
612
5.0%
10639
87.4%
1036
8.5 %
843
6.9 %
82
0.7 %
England - Edge
115
12176
43.4%
612
5.0%
10639
87.4%
1036
8.5 %
843
6.9 %
82
0.7 %
England - LRA
Parallel New Infection
6
735
2.6%
25
3.4%
631
85.9%
51
6.9 %
43
5.9 %
36
4.9 %
England - LRA
6
735
2.6%
25
3.4%
631
85.9%
51
6.9 %
43
5.9 %
36
4.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
11
279
1.0%
26
9.3%
229
82.1%
29
10.4 %
24
8.6 %
0
Parallel - Herd or Group Slaughter
18
1037
3.7%
65
6.3%
888
85.6%
89
8.6 %
81
7.8 %
3
0.3 %
Parallel New Infection
3
39
0.1%
5
12.8%
30
76.9%
4
10.3 %
4
10.3 %
0
Parallel - Other
3
173
0.6%
10
5.8%
147
85.0%
24
13.9 %
16
9.2 %
0
Parallel Persistent Infection
31
2684
9.6%
223
8.3%
2235
83.3%
264
9.8 %
225
8.4 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
36
103
0.4%
33
32.0%
59
57.3%
11
10.7 %
11
10.7 %
0
Wales - High TB
102
4315
15.4%
362
8.4%
3588
83.2%
421
9.8 %
361
8.4 %
4
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
6
168
0.6%
15
8.9%
139
82.7%
18
10.7 %
14
8.3 %
0
Parallel - Herd or Group Slaughter
1
5
0.0%
1
20.0%
3
60.0%
1
20.0 %
1
20.0 %
0
Parallel New Infection
13
712
2.5%
34
4.8%
631
88.6%
55
7.7 %
47
6.6 %
0
Parallel - Other
3
55
0.2%
16
29.1%
38
69.1%
3
5.5 %
1
1.8 %
0
Parallel - Rapid Testing Two-times IRs
4
17
0.1%
4
23.5%
12
70.6%
1
5.9 %
1
5.9 %
0
Wales - Intermediate TB
27
957
3.4%
70
7.3%
823
86.0%
78
8.2 %
64
6.7 %
0
Wales - Low TB
Parallel New Infection
3
57
0.2%
4
7.0%
49
86.0%
4
7.0 %
4
7.0 %
0
Wales - Low TB
3
57
0.2%
4
7.0%
49
86.0%
4
7.0 %
4
7.0 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
39
1147
2.1%
104
9.1%
884
77.1%
86
7.5 %
61
5.3 %
98
8.5 %
Parallel - Herd or Group Slaughter
35
1676
3.0%
116
6.9%
1454
86.8%
121
7.2 %
101
6.0 %
5
0.3 %
Parallel New Infection
261
27807
50.1%
1221
4.4%
24014
86.4%
2167
7.8 %
1843
6.6 %
729
2.6 %
Parallel - new infection cull area
62
7813
14.1%
490
6.3%
6642
85.0%
580
7.4 %
503
6.4 %
178
2.3 %
Parallel - Other
21
1909
3.4%
132
6.9%
1603
84.0%
161
8.4 %
132
6.9 %
42
2.2 %
Parallel Persistent Infection
90
9433
17.0%
699
7.4%
8050
85.3%
687
7.3 %
565
6.0 %
119
1.3 %
Parallel - persistent infection cull area
18
5463
9.8%
341
6.2%
4778
87.5%
407
7.5 %
338
6.2 %
6
0.1 %
Parallel - Rapid Testing Two-times IRs
81
293
0.5%
87
29.7%
166
56.7%
29
9.9 %
22
7.5 %
18
6.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
609
55557
100.0%
3190
5.7%
47607
85.7%
4238
7.6 %
3565
6.4 %
1195
2.2 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
7
329
0.6%
36
10.9%
241
73.3%
23
7.0 %
7
2.1 %
45
13.7 %
Parallel New Infection
3
476
0.9%
74
15.5%
353
74.2%
55
11.6 %
49
10.3 %
0
Parallel - new infection cull area
62
7813
14.1%
490
6.3%
6642
85.0%
580
7.4 %
503
6.4 %
178
2.3 %
Parallel - Other
9
804
1.4%
74
9.2%
639
79.5%
58
7.2 %
49
6.1 %
42
5.2 %
Parallel Persistent Infection
21
2210
4.0%
245
11.1%
1711
77.4%
179
8.1 %
151
6.8 %
103
4.7 %
Parallel - persistent infection cull area
18
5463
9.8%
341
6.2%
4778
87.5%
407
7.5 %
338
6.2 %
6
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - Edge
Parallel New Infection
211
24938
44.9%
1049
4.2%
21583
86.5%
1932
7.7 %
1639
6.6 %
667
2.7 %
Parallel Persistent Infection
4
262
0.5%
14
5.3%
236
90.1%
14
5.3 %
12
4.6 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - LRA
Parallel New Infection
13
989
1.8%
37
3.7%
850
85.9%
76
7.7 %
66
6.7 %
36
3.6 %
England
350
43300
77.9%
2360
5.5%
37049
85.6%
3324
7.7 %
2814
6.5 %
1077
2.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
18
402
0.7%
34
8.5%
338
84.1%
35
8.7 %
30
7.5 %
0
Parallel - Herd or Group Slaughter
32
1664
3.0%
113
6.8%
1447
87.0%
119
7.2 %
99
5.9 %
5
0.3 %
Parallel New Infection
5
46
0.1%
5
10.9%
35
76.1%
6
13.0 %
6
13.0 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Parallel - Other
8
1030
1.9%
40
3.9%
914
88.7%
94
9.1 %
76
7.4 %
0
Parallel Persistent Infection
64
6955
12.5%
440
6.3%
6097
87.7%
494
7.1 %
402
5.8 %
16
0.2 %
Parallel - Rapid Testing Two-times IRs
76
274
0.5%
83
30.3%
152
55.5%
28
10.2 %
21
7.7 %
18
6.6 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
14
416
0.7%
34
8.2%
305
73.3%
28
6.7 %
24
5.8 %
53
12.7 %
Parallel - Herd or Group Slaughter
3
12
0.0%
3
25.0%
7
58.3%
2
16.7 %
2
16.7 %
0
Parallel New Infection
20
1227
2.2%
52
4.2%
1087
88.6%
92
7.5 %
78
6.4 %
10
0.8 %
Parallel - Other
4
75
0.1%
18
24.0%
50
66.7%
9
12.0 %
7
9.3 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
5
19
0.0%
4
21.1%
14
73.7%
1
5.3 %
1
5.3 %
0
Wales - Low TB
Parallel New Infection
9
131
0.2%
4
3.1%
106
80.9%
6
4.6 %
5
3.8 %
16
12.2 %
Wales
259
12257
22.1%
830
6.8%
10558
86.1%
914
7.5 %
751
6.1 %
118
1.0 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 March
189
17292
131
1199
1
235
0
0
0
0
121
6
0
4
0
984
0
72
1
2
April
195
20517
118
953
4
358
0
0
0
0
116
1
0
0
0
748
0
66
1
1
May
250
21066
131
947
2
173
0
0
0
0
121
1
0
4
0
780
0
35
1
1
June
187
18891
110
701
2
184
0
0
0
0
78
2
0
12
0
579
0
25
0
0
July
207
24111
119
806
1
241
0
0
0
0
67
4
0
2
0
675
3
38
0
2
August
228
19599
120
682
4
161
0
0
0
0
64
2
0
8
1
569
0
31
0
0
September
216
21441
137
946
6
348
0
0
0
0
33
1
0
2
0
840
0
51
1
4
October
274
23976
152
1417
12
418
0
0
0
0
99
2
0
3
0
1231
0
44
0
2
November
282
24570
192
1580
2
667
0
0
0
0
42
3
2
8
2
1329
0
167
0
12
December
228
21554
147
1241
6
393
0
0
0
0
92
0
1
7
2
1037
2
74
0
11
2019 January
289
27513
179
1283
5
383
0
0
0
0
85
0
2
1
3
1111
53
10
0
0
February
320
28044
216
1907
48
1109
0
0
0
0
46
0
5
0
0
928
50
0
0
0
Totals (last
12 months)
2865
268574
1752
13662
93
4670
0
0
0
0
964
22
10
51
8
10811
108
613
4
35
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1609
207166
1110
10373
2890
0
0
0
0
797
12
8
30
3
8636
15
72
1
3
Scotland
23
1615
15
128
40
0
0
0
0
22
4
0
0
0
49
0
36
0
2
Wales
1233
59793
627
3161
1740
0
0
0
0
145
6
2
21
5
2126
93
505
3
30
Totals
2865
268574
1752
13662
4670
0
0
0
0
964
22
10
51
8
10811
108
613
4
35
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
0
50
100
150
200
250
300
350
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
0
5000
10000
15000
20000
25000
30000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
Warks
Somerset
Powys
Oxon
Notts
NE Wales
Leics & Rut
Hampshire
Gwent
E Sussex
Devon
Corn & Scilly
Ceredigion
Cambs
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during February 2019 (% submission per
County)
County
%Resampled %Retested
Avon
14.29 %
14.29 %
Berks
8.14 %
9.89 %
Bucks
26.09 %
26.09 %
Cambs
1.82 %
2.42 %
Carms
8.10 %
9.44 %
Ceredigion
10.40 %
11.86 %
Cheshire
4.65 %
6.06 %
Corn & Scilly
9.86 %
9.69 %
Derbyshire
14.27 %
15.85 %
Devon
5.83 %
7.35 %
Dorset
4.66 %
5.75 %
E Sussex
3.25 %
4.34 %
Gloucs
7.17 %
7.83 %
Gwent
6.35 %
6.73 %
Gwynedd
7.02 %
7.02 %
Hampshire
6.40 %
7.06 %
Heref
15.14 %
17.93 %
Leics & Rut
8.53 %
9.04 %
Lincs
30.00 %
30.00 %
NE Wales
6.59 %
8.08 %
Northants
Notts
2.25 %
N Yorks
6.61 %
7.86 %
Oxon
6.53 %
8.57 %
Pembs
8.40 %
10.18 %
Powys
11.11 %
11.11 %
Shropshire
7.86 %
9.29 %
Somerset
8.40 %
8.40 %
Staffs
5.88 %
5.88 %
Warks
6.66 %
8.75 %
W Glamorgan
20.00 %
20.00 %
Wiltshire
2.56 %
3.69 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22388
1471
6.6%
22715
1471
6.5%
Wales
5155
436
8.5%
5329
436
8.2%
GB
27543
1907
6.9%
28044
1907
6.8%
Table 6a (i): Number of animals and submissions tested in February 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
41359
2360
5.7%
43300
2360
5.5%
Wales
11725
830
7.1%
12257
830
6.8%
GB
53084
3190
6.0%
55557
3190
5.7%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
15261
950
6.2%
15552
950
6.1%
Wales
4941
261
5.3%
5026
261
5.2%
Scotland
213
18
8.5%
213
18
8.5%
GB
20415
1229
6.0%
20791
1229
5.9%
Table 6a (ii): Number of animals and submissions tested in February 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of February
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
29141
1607
5.5%
29772
1607
5.4%
Wales
9619
538
5.6%
10071
538
5.3%
Scotland
618
53
8.6%
626
53
8.5%
GB
39378
2198
5.6%
40469
2198
5.4%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SE
3
37
14
37.84 %
Worcestershire
SER-OTHER
1
5
0
Total
4
42
14
33.33 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples February 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Gloucestershire
PRV-SP
1
4
0
Somerset excl North
PRV-SE
4
41
15
36.59 %
Wiltshire
PRV-SP
1
3
0
Worcestershire
SER-OTHER
1
5
0
Total
7
53
15
28.30 %
Isle of Man
SER-IOM
7
24
0
Isle of Man
SER-IOM
4
5
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
2
125
4
3.2 %
North East Wales
2
22
2
9.1 %
TC0077
Sum:
147
6
4.1 %
Table 8a Number of Cattle Antibody Tests February 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
2
125
4
3.2 %
North East Wales
4
119
7
5.9 %
TC0077
Sum:
244
11
4.5 %
Table 8b Number of Cattle Antibody Tests 2019