Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - March 2022
Source: environment.data.gov.uk (2023-01-25)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2022. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 187
Report Period 1st - 31st March 2022
Issued 10 April 2022

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
March 2022
%
Total 2022
%
Submissions
268
759
Samples
Total (%)
23046
100.00 %
59807
100.00 %
England
19262
83.58 %
48739
81.49 %
Wales
3781
xx-xxxx-xxxx %
11063
18.50 %
Scotland
3
0.01 %
5
0.01 %
Parallel Tests
PAR-CHR
125
0.54 %
701
1.17 %
PAR-CUL-N
1186
5.15 %
5928
9.91 %
PAR-CUL-P
1763
7.65 %
3533
5.91 %
PAR-HERD-S
0
0.00 %
40
0.07 %
PAR-IR-EX
30
0.13 %
86
0.14 %
PAR-IR-SEV
252
1.09 %
497
0.83 %
PAR-NEW-IN
3878
xx-xxxx-xxxx %
9754
16.31 %
PAR-OTHER
304
1.32 %
1443
2.41 %
PAR-PERSIS
3898
xx-xxxx-xxxx %
9052
15.14 %
PAR-POSTM
60
0.26 %
134
0.22 %
PAR-RAPID
66
0.29 %
264
0.44 %
PAR-RECUR
11094
48.14 %
24290
40.61 %
Total(% of all samples)
22656
98.31 %
55722
93.17 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
387
1.68 %
4079
6.82 %
SER-FRAUD
0
0.00 %
SER-NSR
3
0.01 %
4
0.01 %
SER-OTHER
0
0.00 %
2
0.00 %
Total(% of all samples)
390
1.69 %
4085
6.83 %
Total
23046
100.00 %
59807
100.00 %
Retests
Total (% of all samples)
2116
9.18 %
5137
8.59 %
England
xx-xxxx-xxxx
7.32 %
4131
6.91 %
Wales
430
1.87 %
1006
1.68 %
Scotland
Resamples
Total (% of all samples)
1758
7.63 %
4393
7.35 %
England
1395
6.05 %
3546
5.93 %
Wales
363
1.58 %
847
1.42 %
Scotland
Rejects
Total (% of all samples)
162
0.70 %
605
1.01 %
England
139
0.60 %
501
0.84 %
Wales
23
0.10 %
104
0.17 %
Scotland
0
0.00 %
Table 1. Monthly Totals for March 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CHR
125
0.5 %
PAR-CUL-N
1186
5.1 %
PAR-CUL-P
1763
7.6 %
PAR-IR-EX
30
0.1 %
PAR-IR-SEV
252
1.1 %
PAR-NEW-IN
3878
16.8 %
PAR-OTHER
304
1.3 %
PAR-PERSIS
3898
16.9 %
PAR-POSTM
60
0.3 %
PAR-RAPID
66
0.3 %
PAR-RECUR
11094
48.1 %
SER-FLEXI
387
1.7 %
SER-NSR
3
0.0 %
Total
23046
100.00 %
Figure 1. Number of samples submitted in each category during March 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
Argyll & Bute
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Leics & Rut
Herts
Heref
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during March 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
1000
2000
3000
4000
5000
6000
7000
8000
9000
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
1
125
0.54 %
10
8.00 %
106
84.80 %
10
8.00 %
9
7.20 %
0
0.00 %
PAR-CUL-P
2
222
0.96 %
20
9.01 %
173
77.93 %
37
16.67 %
29
13.06 %
0
0.00 %
PAR-RECUR
2
498
2.16 %
36
7.23 %
435
87.35 %
27
5.42 %
23
4.62 %
4
0.80 %
Corn & Scilly
PAR-CUL-N
1
161
0.70 %
14
8.70 %
144
89.44 %
6
3.73 %
3
1.86 %
0
0.00 %
PAR-CUL-P
4
658
2.86 %
25
3.80 %
593
90.12 %
46
6.99 %
40
6.08 %
0
0.00 %
PAR-OTHER
1
102
0.44 %
1
0.98 %
98
96.08 %
4
3.92 %
3
2.94 %
0
0.00 %
PAR-RECUR
8
1,407
6.11 %
91
6.47 %
1185
84.22 %
156
11.09 %
131
9.31 %
0
0.00 %
Devon
PAR-CUL-N
4
498
2.16 %
44
8.84 %
410
82.33 %
46
9.24 %
44
8.84 %
0
0.00 %
PAR-CUL-P
3
783
3.40 %
39
4.98 %
666
85.06 %
86
10.98 %
75
9.58 %
3
0.38 %
PAR-RECUR
16
1,929
8.37 %
109
5.65 %
xx-xxxx-xxxx
85.38 %
194
10.06 %
165
8.55 %
8
0.41 %
Dorset
PAR-RECUR
3
536
2.33 %
32
5.97 %
464
86.57 %
43
8.02 %
40
7.46 %
0
0.00 %
SER-FLEXI
2
43
0.19 %
7
16.28 %
34
79.07 %
2
4.65 %
2
4.65 %
0
0.00 %
Gloucs
PAR-CUL-N
2
150
0.65 %
6
4.00 %
125
83.33 %
22
14.67 %
18
12.00 %
1
0.67 %
Heref
PAR-OTHER
1
14
0.06 %
0
0.00 %
9
64.29 %
5
35.71 %
5
35.71 %
0
0.00 %
PAR-PERSIS
2
19
0.08 %
0
0.00 %
15
78.95 %
4
21.05 %
4
21.05 %
0
0.00 %
PAR-RECUR
8
1,943
8.43 %
72
3.71 %
1762
90.68 %
134
6.90 %
108
5.56 %
1
0.05 %
Shropshire
PAR-OTHER
1
34
0.15 %
0
0.00 %
19
55.88 %
15
44.12 %
15
44.12 %
0
0.00 %
PAR-PERSIS
4
1,576
6.84 %
62
3.93 %
1408
89.34 %
132
8.38 %
106
6.73 %
0
0.00 %
PAR-RECUR
8
1,244
5.40 %
28
2.25 %
1119
89.95 %
106
8.52 %
97
7.80 %
0
0.00 %
Somerset excl N
PAR-OTHER
1
4
0.02 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RECUR
6
1,266
5.49 %
65
5.13 %
1103
87.12 %
120
9.48 %
93
7.35 %
5
0.39 %
SER-FLEXI
1
26
0.11 %
5
19.23 %
19
73.08 %
3
11.54 %
2
7.69 %
0
0.00 %
Staffs
PAR-OTHER
1
49
0.21 %
3
6.12 %
45
91.84 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-RECUR
8
1,758
7.63 %
21
1.19 %
1633
92.89 %
123
7.00 %
102
5.80 %
2
0.11 %
Wiltshire
PAR-CUL-P
1
15
0.07 %
0
0.00 %
13
86.67 %
2
13.33 %
2
13.33 %
0
0.00 %
PAR-OTHER
1
90
0.39 %
9
10.00 %
77
85.56 %
6
6.67 %
4
4.44 %
0
0.00 %
Table 2a. Summary by County for March 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
27
0.12 %
0
0.00 %
17
62.96 %
10
37.04 %
10
37.04 %
0
0.00 %
England - Edge
Berks
PAR-RECUR
1
125
0.54 %
2
1.60 %
118
94.40 %
5
4.00 %
5
4.00 %
0
0.00 %
Bucks
PAR-CUL-N
1
224
0.97 %
10
4.46 %
204
91.07 %
11
4.91 %
10
4.46 %
0
0.00 %
PAR-NEW-IN
1
10
0.04 %
0
0.00 %
8
80.00 %
2
20.00 %
2
20.00 %
0
0.00 %
Cheshire
PAR-CUL-P
2
85
0.37 %
0
0.00 %
56
65.88 %
29
34.12 %
29
34.12 %
0
0.00 %
PAR-PERSIS
2
1,835
7.96 %
43
2.34 %
1706
92.97 %
128
6.98 %
83
4.52 %
3
0.16 %
Derbyshire
PAR-NEW-IN
1
70
0.30 %
1
1.43 %
64
91.43 %
7
10.00 %
5
7.14 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
60
0.26 %
1
1.67 %
53
88.33 %
4
6.67 %
4
6.67 %
2
3.33 %
Leics & Rut
PAR-NEW-IN
3
217
0.94 %
7
3.23 %
193
88.94 %
24
11.06 %
17
7.83 %
0
0.00 %
Northants
PAR-NEW-IN
1
118
0.51 %
6
5.08 %
102
86.44 %
12
10.17 %
10
8.47 %
0
0.00 %
Notts
PAR-NEW-IN
1
93
0.40 %
2
2.15 %
84
90.32 %
8
8.60 %
7
7.53 %
0
0.00 %
Oxon
PAR-NEW-IN
2
469
2.04 %
17
3.62 %
426
90.83 %
34
7.25 %
26
5.54 %
0
0.00 %
PAR-RECUR
1
26
0.11 %
0
0.00 %
19
73.08 %
8
30.77 %
7
26.92 %
0
0.00 %
England - LRA
Beds
PAR-CUL-N
1
153
0.66 %
1
0.65 %
126
82.35 %
32
20.92 %
24
15.69 %
2
1.31 %
PAR-NEW-IN
1
91
0.39 %
1
1.10 %
86
94.51 %
3
3.30 %
3
3.30 %
1
1.10 %
Cambs
PAR-NEW-IN
1
72
0.31 %
1
1.39 %
66
91.67 %
4
5.56 %
4
5.56 %
1
1.39 %
Cumbria
PAR-NEW-IN
2
37
0.16 %
2
5.41 %
32
86.49 %
3
8.11 %
3
8.11 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
140
0.61 %
0
0.00 %
132
94.29 %
10
7.14 %
8
5.71 %
0
0.00 %
Herts
PAR-NEW-IN
2
3
0.01 %
0
0.00 %
1
33.33 %
0
0.00 %
0
0.00 %
2
66.67 %
N Yorks
PAR-NEW-IN
2
257
1.12 %
8
3.11 %
130
50.58 %
20
7.78 %
15
5.84 %
104
40.47 %
England
121
19262
83.58 %
801
4.16 %
xx-xxxx-xxxx
87.88 %
xx-xxxx-xxxx
8.75 %
1395
7.24 %
139
0.72 %
Wales - High TB
Carms
PAR-IR-EX
1
9
0.04 %
4
44.44 %
5
55.56 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
8
63
0.27 %
37
58.73 %
22
34.92 %
5
7.94 %
4
6.35 %
0
0.00 %
PAR-OTHER
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
8
22
0.10 %
8
36.36 %
13
59.09 %
1
4.55 %
1
4.55 %
0
0.00 %
SER-FLEXI
4
63
0.27 %
5
7.94 %
33
52.38 %
27
42.86 %
25
39.68 %
0
0.00 %
Ceredigion
PAR-IR-EX
3
11
0.05 %
5
45.45 %
5
45.45 %
0
0.00 %
0
0.00 %
1
9.09 %
PAR-IR-SEV
4
10
0.04 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gwent
PAR-OTHER
2
6
0.03 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
4
0.02 %
1
25.00 %
1
25.00 %
2
50.00 %
2
50.00 %
0
0.00 %
Pembs
PAR-IR-EX
2
6
0.03 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
23
150
0.65 %
45
30.00 %
100
66.67 %
7
4.67 %
4
2.67 %
1
0.67 %
PAR-PERSIS
5
468
2.03 %
46
9.83 %
369
78.85 %
53
11.32 %
44
9.40 %
9
1.92 %
PAR-RAPID
11
25
0.11 %
11
44.00 %
12
48.00 %
3
12.00 %
2
8.00 %
0
0.00 %
PAR-RECUR
4
144
0.62 %
7
4.86 %
113
78.47 %
26
18.06 %
24
16.67 %
0
0.00 %
SER-FLEXI
4
115
0.50 %
8
6.96 %
96
83.48 %
12
10.43 %
11
9.57 %
0
0.00 %
Powys
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
5
16
0.07 %
1
6.25 %
13
81.25 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-RAPID
5
10
0.04 %
7
70.00 %
1
10.00 %
3
30.00 %
2
20.00 %
0
0.00 %
PAR-RECUR
2
99
0.43 %
6
6.06 %
83
83.84 %
12
12.12 %
10
10.10 %
0
0.00 %
S Wales
PAR-IR-SEV
1
4
0.02 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
140
0.61 %
9
6.43 %
125
89.29 %
7
5.00 %
6
4.29 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
9
0.04 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
1,430
6.20 %
24
1.68 %
1304
91.19 %
115
8.04 %
91
6.36 %
11
0.77 %
PAR-POSTM
8
45
0.20 %
5
11.11 %
37
82.22 %
4
8.89 %
3
6.67 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
1
107
0.46 %
12
11.21 %
92
85.98 %
8
7.48 %
3
2.80 %
0
0.00 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-OTHER
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
92
0.40 %
19
20.65 %
56
60.87 %
21
22.83 %
17
18.48 %
0
0.00 %
Shropshire
PAR-IR-EX
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
11
704
3.05 %
32
4.55 %
564
80.11 %
116
xx-xxxx-xxxx %
108
15.34 %
0
0.00 %
PAR-POSTM
4
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-POSTM
6
9
0.04 %
0
0.00 %
8
88.89 %
1
11.11 %
0
0.00 %
1
11.11 %
Wales
146
3781
xx-xxxx-xxxx %
307
8.12 %
3088
81.67 %
430
11.37 %
363
9.60 %
23
0.61 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Scotland
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Grand Total
Sum:
268
23046
1108
20018
2116
1758
162
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
1
125
0.21 %
10
8.00 %
106
84.80 %
10
8.00 %
9
7.20 %
0
0.00 %
PAR-CUL-N
3
527
0.88 %
26
4.93 %
485
92.03 %
16
3.04 %
15
2.85 %
1
0.19 %
PAR-CUL-P
2
222
0.37 %
20
9.01 %
173
77.93 %
37
16.67 %
29
13.06 %
0
0.00 %
PAR-RECUR
7
909
1.52 %
44
4.84 %
805
88.56 %
63
6.93 %
54
5.94 %
6
0.66 %
Corn & Scilly
PAR-CUL-N
2
163
0.27 %
14
8.59 %
146
89.57 %
7
4.29 %
3
1.84 %
0
0.00 %
PAR-CUL-P
5
684
1.14 %
27
3.95 %
609
89.04 %
54
7.89 %
48
7.02 %
0
0.00 %
PAR-OTHER
1
102
0.17 %
1
0.98 %
98
96.08 %
4
3.92 %
3
2.94 %
0
0.00 %
PAR-PERSIS
8
1,683
2.81 %
73
4.34 %
1491
88.59 %
137
8.14 %
119
7.07 %
0
0.00 %
PAR-RECUR
22
3,229
5.40 %
156
4.83 %
2760
85.48 %
357
11.06 %
312
9.66 %
1
0.03 %
Devon
PAR-CUL-N
9
1,462
2.44 %
103
7.05 %
1222
83.58 %
152
10.40 %
136
9.30 %
1
0.07 %
PAR-CUL-P
6
1,195
2.00 %
47
3.93 %
1049
87.78 %
114
9.54 %
94
7.87 %
5
0.42 %
PAR-PERSIS
7
809
1.35 %
55
6.80 %
691
85.41 %
86
10.63 %
63
7.79 %
0
0.00 %
PAR-RECUR
44
5,385
9.00 %
338
6.28 %
4614
85.68 %
485
9.01 %
422
7.84 %
11
0.20 %
Dorset
PAR-CUL-N
5
1,423
2.38 %
58
4.08 %
1279
89.88 %
100
7.03 %
83
5.83 %
3
0.21 %
PAR-OTHER
2
49
0.08 %
1
2.04 %
47
95.92 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-RECUR
4
968
1.62 %
39
4.03 %
863
89.15 %
71
7.33 %
66
6.82 %
0
0.00 %
SER-FLEXI
2
43
0.07 %
7
16.28 %
34
79.07 %
2
4.65 %
2
4.65 %
0
0.00 %
Gloucs
PAR-CUL-N
6
193
0.32 %
10
5.18 %
153
79.27 %
33
17.10 %
29
15.03 %
1
0.52 %
PAR-CUL-P
1
195
0.33 %
7
3.59 %
170
87.18 %
17
8.72 %
17
8.72 %
1
0.51 %
PAR-RECUR
7
1,811
3.03 %
38
2.10 %
1674
92.44 %
112
6.18 %
98
5.41 %
1
0.06 %
Heref
PAR-OTHER
3
422
0.71 %
22
5.21 %
381
90.28 %
29
6.87 %
19
4.50 %
0
0.00 %
PAR-PERSIS
8
1,130
1.89 %
61
5.40 %
973
86.11 %
104
9.20 %
93
8.23 %
3
0.27 %
PAR-RECUR
14
2,295
3.84 %
85
3.70 %
2084
90.81 %
157
6.84 %
125
5.45 %
1
0.04 %
Shropshire
PAR-CHR
2
576
0.96 %
6
1.04 %
536
93.06 %
38
6.60 %
34
5.90 %
0
0.00 %
PAR-OTHER
3
246
0.41 %
4
1.63 %
221
89.84 %
22
8.94 %
21
8.54 %
0
0.00 %
PAR-PERSIS
4
1,576
2.64 %
62
3.93 %
1408
89.34 %
132
8.38 %
106
6.73 %
0
0.00 %
Table 2b. Summary by County for 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
29
3,397
5.68 %
72
2.12 %
3072
90.43 %
274
8.07 %
252
7.42 %
1
0.03 %
Somerset excl N
PAR-OTHER
3
87
0.15 %
15
17.24 %
65
74.71 %
8
9.20 %
7
8.05 %
0
0.00 %
PAR-PERSIS
1
217
0.36 %
3
1.38 %
200
92.17 %
17
7.83 %
14
6.45 %
0
0.00 %
PAR-RECUR
12
1,703
2.85 %
95
5.58 %
1495
87.79 %
147
8.63 %
108
6.34 %
5
0.29 %
SER-FLEXI
1
26
0.04 %
5
19.23 %
19
73.08 %
3
11.54 %
2
7.69 %
0
0.00 %
Staffs
PAR-CUL-N
2
263
0.44 %
6
2.28 %
214
81.37 %
43
16.35 %
43
16.35 %
0
0.00 %
PAR-CUL-P
1
425
0.71 %
11
2.59 %
401
94.35 %
17
4.00 %
13
3.06 %
0
0.00 %
PAR-OTHER
1
49
0.08 %
3
6.12 %
45
91.84 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-PERSIS
4
739
1.24 %
13
1.76 %
442
59.81 %
63
8.53 %
63
8.53 %
221
29.91 %
PAR-RECUR
15
2,703
4.52 %
71
2.63 %
2423
89.64 %
172
6.36 %
136
5.03 %
73
2.70 %
Wiltshire
PAR-CUL-N
4
312
0.52 %
20
6.41 %
267
85.58 %
27
8.65 %
25
8.01 %
0
0.00 %
PAR-CUL-P
4
727
1.22 %
53
7.29 %
609
83.77 %
54
7.43 %
50
6.88 %
15
2.06 %
PAR-NEW-IN
1
19
0.03 %
1
5.26 %
13
68.42 %
5
26.32 %
5
26.32 %
0
0.00 %
PAR-OTHER
1
90
0.15 %
9
10.00 %
77
85.56 %
6
6.67 %
4
4.44 %
0
0.00 %
PAR-PERSIS
2
70
0.12 %
7
10.00 %
46
65.71 %
20
28.57 %
17
24.29 %
0
0.00 %
PAR-RECUR
3
431
0.72 %
32
7.42 %
360
83.53 %
40
9.28 %
37
8.58 %
2
0.46 %
England - Edge
Berks
PAR-RECUR
1
125
0.21 %
2
1.60 %
118
94.40 %
5
4.00 %
5
4.00 %
0
0.00 %
Bucks
PAR-CUL-N
1
224
0.37 %
10
4.46 %
204
91.07 %
11
4.91 %
10
4.46 %
0
0.00 %
PAR-NEW-IN
2
23
0.04 %
0
0.00 %
20
86.96 %
3
13.04 %
3
13.04 %
0
0.00 %
Cheshire
PAR-CUL-N
3
1,208
2.02 %
26
2.15 %
1122
92.88 %
72
5.96 %
60
4.97 %
0
0.00 %
PAR-CUL-P
2
85
0.14 %
0
0.00 %
56
65.88 %
29
34.12 %
29
34.12 %
0
0.00 %
PAR-NEW-IN
3
412
0.69 %
15
3.64 %
367
89.08 %
33
8.01 %
30
7.28 %
0
0.00 %
PAR-PERSIS
3
1,840
3.08 %
43
2.34 %
1709
92.88 %
131
7.12 %
85
4.62 %
3
0.16 %
PAR-RECUR
1
98
0.16 %
5
5.10 %
88
89.80 %
6
6.12 %
5
5.10 %
0
0.00 %
Derbyshire
PAR-NEW-IN
5
550
0.92 %
14
2.55 %
483
87.82 %
57
10.36 %
51
9.27 %
2
0.36 %
E Sussex
PAR-NEW-IN
2
62
0.10 %
1
1.61 %
55
88.71 %
4
6.45 %
4
6.45 %
2
3.23 %
Hampshire
PAR-NEW-IN
1
6
0.01 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
7
799
1.34 %
30
3.75 %
720
90.11 %
61
7.63 %
49
6.13 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
8
983
1.64 %
21
2.14 %
841
85.55 %
129
13.12 %
121
12.31 %
0
0.00 %
PAR-RECUR
1
114
0.19 %
1
0.88 %
93
81.58 %
11
9.65 %
20
17.54 %
0
0.00 %
Notts
PAR-NEW-IN
1
93
0.16 %
2
2.15 %
84
90.32 %
8
8.60 %
7
7.53 %
0
0.00 %
Oxon
PAR-NEW-IN
10
1,392
2.33 %
75
5.39 %
1215
87.28 %
120
8.62 %
102
7.33 %
0
0.00 %
PAR-PERSIS
2
160
0.27 %
15
9.38 %
136
85.00 %
9
5.63 %
9
5.63 %
0
0.00 %
PAR-RECUR
1
26
0.04 %
0
0.00 %
19
73.08 %
8
30.77 %
7
26.92 %
0
0.00 %
England - LRA
Beds
PAR-CUL-N
1
153
0.26 %
1
0.65 %
126
82.35 %
32
20.92 %
24
15.69 %
2
1.31 %
PAR-NEW-IN
1
91
0.15 %
1
1.10 %
86
94.51 %
3
3.30 %
3
3.30 %
1
1.10 %
Cambs
PAR-NEW-IN
1
72
0.12 %
1
1.39 %
66
91.67 %
4
5.56 %
4
5.56 %
1
1.39 %
Cumbria
PAR-NEW-IN
3
249
0.42 %
4
1.61 %
206
82.73 %
24
9.64 %
21
8.43 %
18
7.23 %
Gr Manchester
PAR-NEW-IN
4
173
0.29 %
0
0.00 %
163
94.22 %
12
6.94 %
10
5.78 %
0
0.00 %
Herts
PAR-NEW-IN
5
253
0.42 %
14
5.53 %
197
77.87 %
41
16.21 %
39
15.42 %
3
1.19 %
Lancashire
SER-NSR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northumb
PAR-NEW-IN
1
248
0.41 %
10
4.03 %
208
83.87 %
19
7.66 %
17
6.85 %
13
5.24 %
N Yorks
PAR-NEW-IN
3
408
0.68 %
9
2.21 %
269
65.93 %
31
7.60 %
26
6.37 %
104
25.49 %
W Sussex
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
2
210
0.35 %
5
2.38 %
178
84.76 %
31
14.76 %
27
12.86 %
0
0.00 %
England
339
48739
81.49 %
2036
4.18 %
42656
87.52 %
4131
8.48 %
3546
7.28 %
501
1.03 %
Wales - High TB
Carms
PAR-IR-EX
1
9
0.02 %
4
44.44 %
5
55.56 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
14
94
0.16 %
37
39.36 %
38
40.43 %
8
8.51 %
6
6.38 %
13
13.83 %
PAR-OTHER
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
16
51
0.09 %
13
25.49 %
30
58.82 %
2
3.92 %
2
3.92 %
6
11.76 %
PAR-RECUR
2
22
0.04 %
5
22.73 %
14
63.64 %
5
22.73 %
3
13.64 %
0
0.00 %
SER-FLEXI
10
1,398
2.34 %
79
5.65 %
1230
87.98 %
101
7.22 %
86
6.15 %
3
0.21 %
Ceredigion
PAR-IR-EX
5
13
0.02 %
5
38.46 %
7
53.85 %
0
0.00 %
0
0.00 %
1
7.69 %
PAR-IR-SEV
11
29
0.05 %
11
37.93 %
12
41.38 %
4
13.79 %
4
13.79 %
2
6.90 %
PAR-RAPID
5
9
0.02 %
3
33.33 %
3
33.33 %
1
11.11 %
1
11.11 %
2
22.22 %
PAR-RECUR
1
31
0.05 %
10
32.26 %
21
67.74 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
2
51
0.09 %
2
3.92 %
47
92.16 %
3
5.88 %
2
3.92 %
0
0.00 %
Gwent
PAR-IR-SEV
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
6
0.01 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
PAR-RAPID
5
9
0.02 %
1
11.11 %
6
66.67 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RECUR
3
70
0.12 %
0
0.00 %
66
94.29 %
6
8.57 %
3
4.29 %
1
1.43 %
Pembs
PAR-IR-EX
10
44
0.07 %
13
29.55 %
30
68.18 %
2
4.55 %
1
2.27 %
0
0.00 %
PAR-IR-SEV
46
307
0.51 %
85
27.69 %
205
66.78 %
15
4.89 %
11
3.58 %
6
1.95 %
PAR-PERSIS
7
601
1.00 %
50
8.32 %
487
81.03 %
64
10.65 %
55
9.15 %
9
1.50 %
PAR-RAPID
46
113
0.19 %
34
30.09 %
72
63.72 %
7
6.19 %
6
5.31 %
1
0.88 %
PAR-RECUR
12
638
1.07 %
26
4.08 %
561
87.93 %
64
10.03 %
48
7.52 %
3
0.47 %
SER-FLEXI
23
1,640
2.74 %
203
12.38 %
1287
78.48 %
136
8.29 %
118
7.20 %
33
2.01 %
Powys
PAR-IR-EX
3
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
10
34
0.06 %
1
2.94 %
27
79.41 %
7
20.59 %
6
17.65 %
0
0.00 %
PAR-OTHER
5
163
0.27 %
9
5.52 %
137
84.05 %
18
11.04 %
17
10.43 %
0
0.00 %
PAR-PERSIS
1
36
0.06 %
1
2.78 %
31
86.11 %
5
13.89 %
4
11.11 %
0
0.00 %
PAR-POSTM
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
15
33
0.06 %
11
33.33 %
20
60.61 %
3
9.09 %
2
6.06 %
0
0.00 %
PAR-RECUR
4
107
0.18 %
6
5.61 %
90
84.11 %
14
13.08 %
11
10.28 %
0
0.00 %
S Wales
PAR-IR-SEV
1
4
0.01 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.01 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
4
100.00 %
PAR-OTHER
1
2
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
2
100.00 %
PAR-RAPID
3
7
0.01 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
140
0.23 %
9
6.43 %
125
89.29 %
7
5.00 %
6
4.29 %
0
0.00 %
Ceredigion
PAR-RAPID
1
5
0.01 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
4
8
0.01 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
23
0.04 %
5
21.74 %
18
78.26 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
12
1,876
3.14 %
29
1.55 %
1729
92.16 %
132
7.04 %
107
5.70 %
11
0.59 %
PAR-PERSIS
3
191
0.32 %
3
1.57 %
169
88.48 %
18
9.42 %
18
9.42 %
1
0.52 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-POSTM
16
61
0.10 %
6
9.84 %
51
83.61 %
5
8.20 %
4
6.56 %
0
0.00 %
PAR-RAPID
8
14
0.02 %
5
35.71 %
9
64.29 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
397
0.66 %
33
8.31 %
347
87.41 %
17
4.28 %
16
4.03 %
1
0.25 %
Powys
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
584
0.98 %
31
5.31 %
522
89.38 %
38
6.51 %
29
4.97 %
2
0.34 %
PAR-OTHER
2
214
0.36 %
8
3.74 %
182
85.05 %
28
13.08 %
24
11.21 %
0
0.00 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-OTHER
3
9
0.02 %
3
33.33 %
5
55.56 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-RAPID
2
9
0.02 %
7
77.78 %
2
22.22 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
115
0.19 %
26
22.61 %
72
62.61 %
21
18.26 %
17
14.78 %
0
0.00 %
Shropshire
PAR-IR-EX
2
5
0.01 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
2
113
0.19 %
3
2.65 %
101
89.38 %
9
7.96 %
9
7.96 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
21
1,106
1.85 %
56
5.06 %
879
79.48 %
192
17.36 %
171
15.46 %
0
0.00 %
PAR-POSTM
11
26
0.04 %
0
0.00 %
25
96.15 %
1
3.85 %
1
3.85 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-HERD-S
2
40
0.07 %
4
10.00 %
28
70.00 %
8
20.00 %
8
20.00 %
0
0.00 %
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
144
0.24 %
0
0.00 %
132
91.67 %
15
10.42 %
10
6.94 %
2
1.39 %
PAR-POSTM
24
44
0.07 %
2
4.55 %
40
90.91 %
3
6.82 %
1
2.27 %
1
2.27 %
PAR-RAPID
2
9
0.02 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
2
384
0.64 %
38
9.90 %
312
81.25 %
40
10.42 %
34
8.85 %
0
0.00 %
Wales
418
11063
18.50 %
890
8.04 %
9223
83.37 %
1006
9.09 %
847
7.66 %
104
0.94 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Scotland
2
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Grand Total
Sum:
759
59807
2926
51884
5137
4393
605
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol March 2022
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
118
19193
99.6%
789
4.1%
xx-xxxx-xxxx
87.9%
xx-xxxx-xxxx
8.8%
1391
7.2%
139
0.7%
Serial/Extended
3
69
0.4%
12
17.4%
53
76.8%
5
7.2%
4
5.8%
0
0.0 %
Total
121
19262
100.0%
801
4.2%
xx-xxxx-xxxx
87.9%
xx-xxxx-xxxx
8.8%
1395
7.2%
139
0.7%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
1
3
100.0%
0
0.0 %
3 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
3
100.0%
0
0.0 %
3 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
137
3463
91.6%
285
8.2%
2834
81.8%
384
11.1%
321
9.3%
23
0.7%
Serial/Extended
9
318
8.4%
22
6.9%
254
79.9%
46
14.5%
42
13.2%
0
0.0 %
Total
146
3781
100.0%
307
8.1%
3088
81.7%
430
11.4%
363
9.6%
23
0.6%
GB
Parallel
255
22656
98.3%
1074
4.7%
19708
87.0%
2065
9.1%
1712
7.6%
162
0.7%
Serial/Extended
13
390
1.7%
34
8.7%
310
79.5%
51
13.1%
46
11.8%
0
0.0 %
Total
268
23046
100.0%
1108
4.8%
20018
86.9%
2116
9.2%
1758
7.6%
162
0.7%
121.00
146.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2022)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
335
48669
99.9%
2024
4.2%
42602
87.5%
4126
8.5%
3542
7.3%
501
1.0%
Serial/Extended
4
70
0.1%
12
17.1%
54
77.1%
5
7.1%
4
5.7%
0
0.0 %
Total
339
48739
100.0%
2036
4.2%
42656
87.5%
4131
8.5%
3546
7.3%
501
1.0%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
2
5
100.0%
0
0.0 %
5 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
5
100.0%
0
0.0 %
5 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
373
7053
63.8%
526
7.5%
5875
83.3%
702
10.0%
585
8.3%
67
0.9%
Serial/Extended
45
4010
36.2%
364
9.1%
3348
83.5%
304
7.6%
262
6.5%
37
0.9%
Total
418
11063
100.0%
890
8.0%
9223
83.4%
1006
9.1%
847
7.7%
104
0.9%
GB
Parallel
708
55722
93.2%
2550
4.6%
48477
87.0%
4828
8.7%
4127
7.4%
568
1.0%
Serial/Extended
51
4085
6.8%
376
9.2%
3407
83.4%
309
7.6%
266
6.5%
37
0.9%
Total
759
59807
100.0%
2926
4.9%
51884
86.8%
5137
8.6%
4393
7.3%
605
1.0%
339.00
418.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
12
387
1.7%
34
8.8%
307
79.3%
51
13.2 %
46
11.9 %
0
Parallel – chronic breakdown
1
125
0.5%
10
8.0%
106
84.8%
10
8.0 %
9
7.2 %
0
Parallel - IRs in Exit Strategy for persistent her
10
30
0.1%
10
33.3%
18
60.0%
1
3.3 %
1
3.3 %
1
3.3 %
Parallel New Infection
36
3878
16.8%
114
2.9%
3337
86.0%
370
9.5 %
306
7.9 %
121
3.1 %
Parallel - new infection cull area
9
1186
5.1%
75
6.3%
1009
85.1%
117
9.9 %
99
8.3 %
3
0.3 %
Parallel - Other
10
304
1.3%
15
4.9%
259
85.2%
34
11.2 %
30
9.9 %
0
Parallel Persistent Infection
13
3898
16.9%
151
3.9%
3498
89.7%
317
8.1 %
237
6.1 %
12
0.3 %
Parallel - persistent infection cull area
12
1763
7.6%
84
4.8%
1501
85.1%
200
11.3 %
175
9.9 %
3
0.2 %
Parallel – post-mvt test coupled with IDEXX an
19
60
0.3%
5
8.3%
51
85.0%
5
8.3 %
3
5.0 %
1
1.7 %
Parallel - Rapid Testing Two-times IRs
32
66
0.3%
30
45.5%
29
43.9%
9
13.6 %
7
10.6 %
0
Parallel - Recurrent breakdowns
70
11094
48.1%
488
4.4%
9754
87.9%
985
8.9 %
832
7.5 %
20
0.2 %
Parallel - severe IRs - in persistent herds
43
252
1.1%
92
36.5%
146
57.9%
17
6.7 %
13
5.2 %
1
0.4 %
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Total
268
23046
100.0%
1108
4.8%
20018
86.9%
2116
9.2 %
1758
7.6 %
162
0.7 %
Table 4a. Summaryof IFN Gamma results by submission reason (March 2022)
England - HRA
Extended Flexible IFN-GAMMA test
3
69
0.3%
12
17.4%
53
76.8%
5
7.2 %
4
5.8 %
0
Parallel – chronic breakdown
1
125
0.5%
10
8.0%
106
84.8%
10
8.0 %
9
7.2 %
0
Parallel - new infection cull area
7
809
3.5%
64
7.9%
679
83.9%
74
9.1 %
65
8.0 %
1
0.1 %
Parallel - Other
6
293
1.3%
13
4.4%
250
85.3%
33
11.3 %
30
10.2 %
0
Parallel Persistent Infection
6
1595
6.9%
62
3.9%
1423
89.2%
136
8.5 %
110
6.9 %
0
Parallel - persistent infection cull area
10
1678
7.3%
84
5.0%
1445
86.1%
171
10.2 %
146
8.7 %
3
0.2 %
Parallel - Recurrent breakdowns
60
10608
46.0%
454
4.3%
9365
88.3%
913
8.6 %
769
7.2 %
20
0.2 %
England - HRA
93
15177
65.9%
699
4.6%
13321
87.8%
1342
8.8 %
1133
7.5 %
24
0.2 %
England - Edge
Parallel New Infection
10
1037
4.5%
34
3.3%
930
89.7%
91
8.8 %
71
6.8 %
2
0.2 %
Parallel - new infection cull area
1
224
1.0%
10
4.5%
204
91.1%
11
4.9 %
10
4.5 %
0
Parallel Persistent Infection
2
1835
8.0%
43
2.3%
1706
93.0%
128
7.0 %
83
4.5 %
3
0.2 %
Parallel - persistent infection cull area
2
85
0.4%
0
0.0 %
56
65.9%
29
34.1 %
29
34.1 %
0
Parallel - Recurrent breakdowns
2
151
0.7%
2
1.3%
137
90.7%
13
8.6 %
12
7.9 %
0
England - Edge
17
3332
14.5%
89
2.7%
3033
91.0%
272
8.2 %
205
6.2 %
5
0.2 %
England - LRA
Parallel New Infection
10
600
2.6%
12
2.0%
447
74.5%
40
6.7 %
33
5.5 %
108
18.0 %
Parallel - new infection cull area
1
153
0.7%
1
0.7%
126
82.4%
32
20.9 %
24
15.7 %
2
1.3 %
England - LRA
11
753
3.3%
13
1.7%
573
76.1%
72
9.6 %
57
7.6 %
110
14.6 %
Wales - High TB
Extended Flexible IFN-GAMMA test
8
178
0.8%
13
7.3%
129
72.5%
39
21.9 %
36
20.2 %
0
Parallel - IRs in Exit Strategy for persistent her
7
27
0.1%
10
37.0%
16
59.3%
0
0
1
3.7 %
Parallel - Other
3
10
0.0%
1
10.0%
9
90.0%
1
10.0 %
0
0
Parallel Persistent Infection
5
468
2.0%
46
9.8%
369
78.8%
53
11.3 %
44
9.4 %
9
1.9 %
Parallel - Rapid Testing Two-times IRs
27
61
0.3%
27
44.3%
27
44.3%
9
14.8 %
7
11.5 %
0
Parallel - Recurrent breakdowns
6
243
1.1%
13
5.3%
196
80.7%
38
15.6 %
34
14.0 %
0
Parallel - severe IRs - in persistent herds
41
243
1.1%
90
37.0%
139
57.2%
17
7.0 %
13
5.3 %
1
0.4 %
Wales - High TB
97
1230
5.3%
200
16.3%
885
72.0%
157
12.8 %
134
10.9 %
11
0.9 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
140
0.6%
9
6.4%
125
89.3%
7
5.0 %
6
4.3 %
0
Parallel - IRs in Exit Strategy for persistent her
2
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel New Infection
5
1537
6.7%
36
2.3%
1396
90.8%
123
8.0 %
94
6.1 %
11
0.7 %
Parallel - Other
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Parallel – post-mvt test coupled with IDEXX an
9
46
0.2%
5
10.9%
38
82.6%
4
8.7 %
3
6.5 %
0
Parallel - Rapid Testing Two-times IRs
4
4
0.0%
3
75.0%
1
25.0%
0
0
0
Parallel - Recurrent breakdowns
2
92
0.4%
19
20.7%
56
60.9%
21
22.8 %
17
18.5 %
0
Parallel - severe IRs - in persistent herds
2
9
0.0%
2
22.2%
7
77.8%
0
0
0
Wales - Intermediate TB
26
1831
7.9%
75
4.1%
1624
88.7%
156
8.5 %
121
6.6 %
11
0.6 %
Wales - Low TB
Parallel - IRs in Exit Strategy for persistent her
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
11
704
3.1%
32
4.5%
564
80.1%
116
16.5 %
108
15.3 %
0
Parallel – post-mvt test coupled with IDEXX an
10
14
0.1%
0
0.0 %
13
92.9%
1
7.1 %
0
1
7.1 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
23
720
3.1%
32
4.4%
579
80.4%
117
16.3 %
108
15.0 %
1
0.1 %
Scotland - Scotland
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Scotland - Scotland
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
48
4079
6.8%
376
9.2%
3401
83.4%
309
7.6 %
266
6.5 %
37
0.9 %
Parallel – chronic breakdown
3
701
1.2%
16
2.3%
642
91.6%
48
6.8 %
43
6.1 %
0
Parallel - Herd or Group Slaughter
2
40
0.1%
4
10.0%
28
70.0%
8
20.0 %
8
20.0 %
0
Parallel - IRs in Exit Strategy for persistent herds
27
86
0.1%
23
26.7%
59
68.6%
4
4.7 %
3
3.5 %
1
1.2 %
Parallel New Infection
104
9754
16.3%
320
3.3%
8439
86.5%
962
9.9 %
836
8.6 %
159
1.6 %
Parallel - new infection cull area
36
5928
9.9%
274
4.6%
5218
88.0%
493
8.3 %
428
7.2 %
8
0.1 %
Parallel - Other
28
1443
2.4%
76
5.3%
1267
87.8%
119
8.2 %
98
6.8 %
2
0.1 %
Parallel Persistent Infection
50
9052
15.1%
386
4.3%
7783
86.0%
786
8.7 %
646
7.1 %
237
2.6 %
Parallel - persistent infection cull area
21
3533
5.9%
165
4.7%
3067
86.8%
322
9.1 %
280
7.9 %
21
0.6 %
Parallel – post-mvt test coupled with IDEXX anti
53
134
0.2%
8
6.0%
119
88.8%
9
6.7 %
6
4.5 %
1
0.7 %
Parallel - Rapid Testing Two-times IRs
106
264
0.4%
81
30.7%
160
60.6%
16
6.1 %
14
5.3 %
9
3.4 %
Parallel - Recurrent breakdowns
188
24290
40.6%
1054
4.3%
21393
88.1%
2027
8.3 %
1738
7.2 %
105
0.4 %
Parallel - severe IRs - in persistent herds
90
497
0.8%
143
28.8%
302
60.8%
34
6.8 %
27
5.4 %
25
5.0 %
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
2
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Total
759
59807
100.0%
2926
4.9%
51884
86.8%
5137
8.6 %
4393
7.3 %
605
1.0 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2022)
England - HRA
Extended Flexible IFN-GAMMA test
3
69
0.1%
12
17.4%
53
76.8%
5
7.2 %
4
5.8 %
0
Parallel – chronic breakdown
3
701
1.2%
16
2.3%
642
91.6%
48
6.8 %
43
6.1 %
0
Parallel New Infection
1
19
0.0%
1
5.3%
13
68.4%
5
26.3 %
5
26.3 %
0
Parallel - new infection cull area
31
4343
7.3%
237
5.5%
3766
86.7%
378
8.7 %
334
7.7 %
6
0.1 %
Parallel - Other
14
1045
1.7%
55
5.3%
934
89.4%
71
6.8 %
56
5.4 %
0
Parallel Persistent Infection
34
6224
10.4%
274
4.4%
5251
84.4%
559
9.0 %
475
7.6 %
224
3.6 %
Parallel - persistent infection cull area
19
3448
5.8%
165
4.8%
3011
87.3%
293
8.5 %
251
7.3 %
21
0.6 %
Parallel - Recurrent breakdowns
157
22831
38.2%
970
4.2%
20150
88.3%
1878
8.2 %
1610
7.1 %
101
0.4 %
England - Edge
Parallel New Infection
39
4320
7.2%
159
3.7%
3790
87.7%
415
9.6 %
367
8.5 %
4
0.1 %
Parallel - new infection cull area
4
1432
2.4%
36
2.5%
1326
92.6%
83
5.8 %
70
4.9 %
0
Parallel Persistent Infection
5
2000
3.3%
58
2.9%
1845
92.3%
140
7.0 %
94
4.7 %
3
0.2 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
Parallel - persistent infection cull area
2
85
0.1%
0
0.0 %
56
65.9%
29
34.1 %
29
34.1 %
0
Parallel - Recurrent breakdowns
4
363
0.6%
8
2.2%
318
87.6%
30
8.3 %
37
10.2 %
0
England - LRA
Parallel New Infection
21
1705
2.9%
44
2.6%
1374
80.6%
165
9.7 %
147
8.6 %
140
8.2 %
Parallel - new infection cull area
1
153
0.3%
1
0.7%
126
82.4%
32
20.9 %
24
15.7 %
2
1.3 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
England
339
48739
81.5%
2036
4.2%
42656
87.5%
4131
8.5 %
3546
7.3 %
501
1.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
35
3089
5.2%
284
9.2%
2564
83.0%
240
7.8 %
206
6.7 %
36
1.2 %
Parallel - IRs in Exit Strategy for persistent herd
19
71
0.1%
22
31.0%
47
66.2%
2
2.8 %
1
1.4 %
1
1.4 %
Parallel - Other
8
173
0.3%
10
5.8%
146
84.4%
19
11.0 %
17
9.8 %
0
Parallel Persistent Infection
8
637
1.1%
51
8.0%
518
81.3%
69
10.8 %
59
9.3 %
9
1.4 %
Parallel – post-mvt test coupled with IDEXX ant
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
87
215
0.4%
62
28.8%
131
60.9%
15
7.0 %
13
6.0 %
9
4.2 %
Parallel - Recurrent breakdowns
22
868
1.5%
47
5.4%
752
86.6%
89
10.3 %
65
7.5 %
4
0.5 %
Parallel - severe IRs - in persistent herds
83
470
0.8%
138
29.4%
284
60.4%
34
7.2 %
27
5.7 %
21
4.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
8
537
0.9%
42
7.8%
472
87.9%
24
4.5 %
22
4.1 %
1
0.2 %
Parallel - IRs in Exit Strategy for persistent herd
7
14
0.0%
1
7.1%
11
78.6%
2
14.3 %
2
14.3 %
0
Parallel New Infection
18
2460
4.1%
60
2.4%
2251
91.5%
170
6.9 %
136
5.5 %
13
0.5 %
Parallel - Other
6
225
0.4%
11
4.9%
187
83.1%
29
12.9 %
25
11.1 %
2
0.9 %
Parallel Persistent Infection
3
191
0.3%
3
1.6%
169
88.5%
18
9.4 %
18
9.4 %
1
0.5 %
Parallel – post-mvt test coupled with IDEXX ant
17
62
0.1%
6
9.7%
52
83.9%
5
8.1 %
4
6.5 %
0
Parallel - Rapid Testing Two-times IRs
16
39
0.1%
18
46.2%
21
53.8%
0
0
0
Parallel - Recurrent breakdowns
5
228
0.4%
29
12.7%
173
75.9%
30
13.2 %
26
11.4 %
0
Parallel - severe IRs - in persistent herds
7
27
0.0%
5
18.5%
18
66.7%
0
0
4
14.8 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
384
0.6%
38
9.9%
312
81.3%
40
10.4 %
34
8.9 %
0
Parallel - Herd or Group Slaughter
2
40
0.1%
4
10.0%
28
70.0%
8
20.0 %
8
20.0 %
0
Parallel - IRs in Exit Strategy for persistent herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
25
1250
2.1%
56
4.5%
1011
80.9%
207
16.6 %
181
14.5 %
2
0.2 %
Parallel – post-mvt test coupled with IDEXX ant
35
70
0.1%
2
2.9%
65
92.9%
4
5.7 %
2
2.9 %
1
1.4 %
Parallel - Rapid Testing Two-times IRs
3
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Wales
418
11063
18.5%
890
8.0%
9223
83.4%
1006
9.1 %
847
7.7 %
104
0.9 %
Scotland - Scotland
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
Scotland
2
5
0.0%
0
0.0 %
5
100.0%
0
0
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2021 April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
4
0
9
0
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
4
0
4
0
1083
0
92
0
6
July
298
24841
164
1077
30
0
0
0
0
61
4
0
4
0
936
0
40
0
2
August
312
17920
167
912
3
26
0
0
0
0
35
2
0
7
0
755
0
81
1
2
September
274
15805
130
890
13
0
0
0
0
95
1
0
7
0
742
0
31
0
0
October
276
19790
140
814
15
0
0
0
0
44
3
2
17
0
696
0
36
1
0
November
329
25737
195
1349
26
0
0
0
0
92
1
3
22
1
1143
1
60
0
0
December
238
13117
138
759
14
0
0
0
0
42
0
2
1
5
656
1
35
0
2
2022 January
262
18563
129
852
23
0
0
0
0
33
0
0
17
1
740
8
29
0
0
February
229
18198
132
966
1
34
0
0
0
0
84
0
6
0
0
757
84
0
0
0
March
257
22312
158
1062
291
369
0
0
0
0
13
0
2
0
0
93
1
0
0
0
Totals (last
12 months)
3554
259401
1986
13130
299
669
0
0
0
0
839
22
15
101
7
10159
95
610
4
13
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
xx-xxxx-xxxx
203315
1098
9393
545
0
0
0
0
745
9
10
52
6
7526
0
60
0
0
Scotland
17
1600
12
115
0
0
0
0
0
47
0
0
0
0
53
0
10
0
5
Wales
1848
54486
876
3622
124
0
0
0
0
47
13
5
49
1
2580
95
540
4
8
Totals
3554
259401
1986
13130
669
0
0
0
0
839
22
15
101
7
10159
95
610
4
13
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2020
2021
2022
IFNg Positive herds
IFNg Negative herds
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2020
2021
2022
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2020
2021
2022
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
Wiltshire
Staffs
Shropshire
Powys
Oxon
Notts
NE Wales
Herts
Gwynedd
Gr Manchester
E Sussex
Devon
Cumbria
Cheshire
Carms
Bucks
Beds
Argyll & Bute
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during March 2022 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
Avon
7.22 %
8.76 %
Beds
11.07 %
14.34 %
Berks
4.00 %
4.00 %
Bucks
5.13 %
5.56 %
Cambs
5.56 %
5.56 %
Carms
11.92 %
13.25 %
Ceredigion
14.29 %
14.29 %
Cheshire
5.83 %
8.18 %
Corn & Scilly
7.60 %
9.11 %
Cumbria
8.11 %
8.11 %
Derbyshire
7.14 %
10.00 %
Devon
8.85 %
10.16 %
Dorset
7.25 %
7.77 %
E Sussex
6.67 %
6.67 %
Gloucs
12.00 %
14.67 %
Gr Manchester
5.71 %
7.14 %
Gwent
20.00 %
30.00 %
Gwynedd
15.21 %
16.34 %
Heref
5.92 %
7.24 %
Herts
Leics & Rut
7.83 %
11.06 %
NE Wales
6.28 %
8.02 %
Northants
8.47 %
10.17 %
Notts
7.53 %
8.60 %
N Yorks
5.84 %
7.78 %
Oxon
6.67 %
8.48 %
Pembs
9.36 %
11.12 %
Powys
7.23 %
10.64 %
S Glamorgan
18.28 %
22.58 %
Shropshire
7.67 %
8.90 %
Somerset excl
7.48 %
9.65 %
Staffs
5.70 %
6.86 %
S Wales
Wiltshire
12.12 %
13.64 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18807
801
4.3%
19262
801
4.2%
Wales
3655
307
8.4%
3781
307
8.1%
Scotland
3
0
0.0 %
3
0.0 %
GB
22465
1108
4.9%
23046
1108
4.8%
Table 6a (i): Number of animals and submissions tested in March 2022
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
46786
2036
4.4%
48739
2036
4.2%
Wales
10463
890
8.5%
11063
890
8.0%
Scotland
5
0
0.0 %
5
0.0 %
GB
57254
2926
5.1%
59807
2926
4.9%
Table 6b (i): Number of animals and submissions tested in 2022 Up to end of March
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
26397
1380
5.2%
26938
1380
5.1%
Wales
9201
487
5.3%
9402
487
5.2%
Scotland
4
0
0.0 %
4
0.0 %
GB
35602
1867
5.2%
36344
1867
5.1%
Table 6a (ii): Number of animals and submissions tested in March 2021
Table 6b (ii): Number of animals and submissions tested in 2021 Up to end of March
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
69243
3939
5.7%
72279
3939
5.4%
Wales
19496
1183
6.1%
20339
1183
5.8%
Scotland
393
35
8.9%
425
35
8.2%
GB
89129
5157
5.8%
93043
5157
5.5%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples March 2022
Table 7b Number of private samples 2022
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Hampshire
PRV-SE
1
7
2
28.57 %
Somerset excl North
PRV-SP
1
4
0
0
Wiltshire
PRV-SE
1
1
0
0
Total
4
14
2
14.29 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
3
41
24
58.5%
BOV-IR-EX
1
10
1
10.0%
BOV-OTHER
1
152
7
4.6%
Ceredigion
BOV-IR-C
3
7
0
0
BOV-IR-EX
3
11
3
27.3%
North East Wales
BOV-IR-C
2
8
1
12.5%
BOV-POSTM
8
13
3
23.1%
North West Wales
BOV-POSTM
1
1
0
0
Pembrokeshire
BOV-IR-C
14
122
27
22.1%
BOV-IR-EX
3
17
7
41.2%
BOV-P
1
94
3
3.2%
Powys
BOV-IR-C
2
10
0
0
BOV-POSTM
1
1
0
0
South Wales
BOV-IR-C
1
4
1
25.0%
BOV-OTHER
1
5
1
20.0%
Wales
45
496
78
15.7%
All GB
45
496
78
15.7 %
Table 8a Number of Cattle Antibody Tests March 2022
Table 8b Number of Cattle Antibody Tests 2022
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Wales
Carmarthenshire
BOV-IR-C
6
72
26
36.1%
BOV-IR-EX
1
10
1
10.0%
BOV-OTHER
1
152
7
4.6%
Ceredigion
BOV-IR-C
8
73
8
11.0%
BOV-IR-EX
3
11
3
27.3%
North East Wales
BOV-IR-C
9
98
15
15.3%
BOV-IR-EX
1
3
1
33.3%
BOV-OTHER
1
17
0
0
BOV-P
1
420
43
10.2%
BOV-POSTM
23
47
13
27.7%
North West Wales
BOV-POSTM
1
1
0
0
Pembrokeshire
BOV-IR-C
34
313
68
21.7%
BOV-IR-EX
7
31
10
32.3%
BOV-OTHER
3
176
6
3.4%
BOV-P
2
209
18
8.6%
Powys
BOV-IR-C
6
38
4
10.5%
BOV-POSTM
1
1
0
0
South Wales
BOV-IR-C
2
8
1
12.5%
BOV-OTHER
1
5
1
20.0%
Wales
111
1,685
225
13.4%
All GB
111
1,685
225
13.4 %