Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - October 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 170
Report Period 1st - 31st October 2020
Issued 10 November 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
October
2020
%
Total 2020
%
Submissions
316
2841
Samples
Total (%)
26668
100.00 %
233717
100.00 %
England
19353
72.57 %
182831
78.23 %
Wales
7315
27.43 %
46356
19.83 %
Scotland
4530
1.94 %
Parallel Tests
PAR-CUL-N
9096
34.11 %
84934
36.34 %
PAR-CUL-P
743
2.79 %
8104
3.47 %
PAR-HERD-S
301
1.13 %
1433
0.61 %
PAR-NEW-IN
11248
42.18 %
100155
42.85 %
PAR-OTHER
1406
5.27 %
6583
2.82 %
PAR-PERSIS
3371
12.64 %
27312
11.69 %
PAR-RAPID
182
0.68 %
1354
0.58 %
Total(% of all samples)
26347
98.80 %
229875
98.36 %
Serial/Extended Tests
SER-FLEXI
321
1.20 %
3823
1.64 %
SER-FRAUD
0
0.00 %
19
0.01 %
SER-NSR
0
0.00 %
Total(% of all samples)
321
1.20 %
3842
1.64 %
Total
26668
100.00 %
233717
100.00 %
Retests
Total (% of all samples)
1575
5.91 %
14086
6.03 %
England
1186
4.45 %
11130
4.76 %
Wales
389
1.46 %
2669
1.14 %
Scotland
287
0.12 %
Resamples
Total (% of all samples)
1283
4.81 %
10789
4.62 %
England
963
3.61 %
8460
3.62 %
Wales
320
1.20 %
2102
0.90 %
Scotland
227
0.10 %
Rejects
Total (% of all samples)
19
0.07 %
2346
1.00 %
England
6
0.02 %
2228
0.95 %
Wales
13
0.05 %
53
0.02 %
Scotland
0
0.00 %
65
0.03 %
Table 1. Monthly Totals for October 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
9096
34.1 %
PAR-CUL-P
743
2.8 %
PAR-HERD-S
301
1.1 %
PAR-NEW-IN
11248
42.2 %
PAR-OTHER
1406
5.3 %
PAR-PERSIS
3371
12.6 %
PAR-RAPID
182
0.7 %
SER-FLEXI
321
1.2 %
Total
26668
100.00 %
Figure 1. Number of samples submitted in each category during October 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
W Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Kent
Heref
Hampshire
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during October 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
Wigtown
Perthshire
Kirkcudbright
Dumfries & G
Berwickshire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Kent
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
9
812
3.04 %
34
4.19 %
728
89.66 %
61
7.51 %
50
6.16 %
0
0.00 %
PAR-PERSIS
1
472
1.77 %
21
4.45 %
437
92.58 %
15
3.18 %
14
2.97 %
0
0.00 %
Devon
PAR-CUL-N
30
2,959
11.10 %
250
8.45 %
2559
86.48 %
173
5.85 %
145
4.90 %
5
0.17 %
PAR-CUL-P
3
500
1.87 %
50
10.00 %
432
86.40 %
22
4.40 %
18
3.60 %
0
0.00 %
PAR-NEW-IN
1
14
0.05 %
1
7.14 %
10
71.43 %
3
21.43 %
3
21.43 %
0
0.00 %
Dorset
PAR-CUL-N
12
979
3.67 %
64
6.54 %
878
89.68 %
44
4.49 %
37
3.78 %
0
0.00 %
PAR-CUL-P
2
242
0.91 %
7
2.89 %
218
90.08 %
20
8.26 %
17
7.02 %
0
0.00 %
PAR-PERSIS
2
19
0.07 %
0
0.00 %
16
84.21 %
3
15.79 %
3
15.79 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
0
0.00 %
2
100.00 %
2
100.00 %
0
0.00 %
Gloucs
PAR-CUL-N
7
700
2.62 %
38
5.43 %
617
88.14 %
57
8.14 %
44
6.29 %
1
0.14 %
Heref
PAR-CUL-N
1
312
1.17 %
12
3.85 %
283
90.71 %
20
6.41 %
17
5.45 %
0
0.00 %
Shropshire
PAR-CUL-N
9
2,339
8.77 %
50
2.14 %
2177
93.07 %
151
6.46 %
112
4.79 %
0
0.00 %
Somerset excl N
PAR-CUL-N
12
546
2.05 %
27
4.95 %
496
90.84 %
32
5.86 %
23
4.21 %
0
0.00 %
PAR-CUL-P
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
Staffs
PAR-CUL-N
3
145
0.54 %
5
3.45 %
121
83.45 %
20
13.79 %
19
13.10 %
0
0.00 %
PAR-NEW-IN
1
359
1.35 %
9
2.51 %
332
92.48 %
19
5.29 %
18
5.01 %
0
0.00 %
Wiltshire
PAR-CUL-N
4
304
1.14 %
15
4.93 %
280
92.11 %
9
2.96 %
9
2.96 %
0
0.00 %
England - Edge
Berks
PAR-PERSIS
1
4
0.01 %
0
0.00 %
1
25.00 %
3
75.00 %
3
75.00 %
0
0.00 %
Bucks
PAR-NEW-IN
1
269
1.01 %
11
4.09 %
211
78.44 %
53
19.70 %
47
17.47 %
0
0.00 %
Cheshire
PAR-NEW-IN
16
3,811
14.29 %
50
1.31 %
3638
95.46 %
166
4.36 %
123
3.23 %
0
0.00 %
Derbyshire
PAR-NEW-IN
8
1,298
4.87 %
80
6.16 %
1112
85.67 %
117
9.01 %
106
8.17 %
0
0.00 %
E Sussex
PAR-NEW-IN
2
76
0.28 %
0
0.00 %
73
96.05 %
4
5.26 %
3
3.95 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
194
0.73 %
16
8.25 %
167
86.08 %
11
5.67 %
11
5.67 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
8
929
3.48 %
22
2.37 %
851
91.60 %
65
7.00 %
56
6.03 %
0
0.00 %
Northants
PAR-NEW-IN
2
452
1.69 %
6
1.33 %
433
95.80 %
14
3.10 %
13
2.88 %
0
0.00 %
Table 2a. Summary by County for October 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Oxon
PAR-NEW-IN
2
304
1.14 %
6
1.97 %
286
94.08 %
21
6.91 %
12
3.95 %
0
0.00 %
Warks
PAR-NEW-IN
9
944
3.54 %
21
2.22 %
884
93.64 %
61
6.46 %
39
4.13 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
14
0.05 %
1
7.14 %
10
71.43 %
3
21.43 %
3
21.43 %
0
0.00 %
Essex
PAR-NEW-IN
3
265
0.99 %
3
1.13 %
252
95.09 %
11
4.15 %
10
3.77 %
0
0.00 %
Kent
PAR-NEW-IN
1
58
0.22 %
1
1.72 %
53
91.38 %
4
6.90 %
4
6.90 %
0
0.00 %
Lincs
PAR-NEW-IN
1
27
0.10 %
0
0.00 %
25
92.59 %
2
7.41 %
2
7.41 %
0
0.00 %
England
156
19353
72.57 %
801
4.14 %
17583
90.85 %
1186
6.13 %
963
4.98 %
6
0.03 %
Wales - High TB
Carms
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
33
0.12 %
22
66.67 %
9
27.27 %
2
6.06 %
2
6.06 %
0
0.00 %
PAR-PERSIS
5
29
0.11 %
3
10.34 %
20
68.97 %
6
20.69 %
6
20.69 %
0
0.00 %
PAR-RAPID
7
34
0.13 %
9
26.47 %
23
67.65 %
2
5.88 %
2
5.88 %
0
0.00 %
Ceredigion
PAR-OTHER
4
329
1.23 %
34
10.33 %
281
85.41 %
16
4.86 %
14
4.26 %
0
0.00 %
PAR-PERSIS
2
249
0.93 %
13
5.22 %
213
85.54 %
23
9.24 %
23
9.24 %
0
0.00 %
PAR-RAPID
4
12
0.04 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
7
356
1.33 %
31
8.71 %
310
87.08 %
21
5.90 %
15
4.21 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
28
0.10 %
3
10.71 %
25
89.29 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-NEW-IN
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
14
826
3.10 %
55
6.66 %
739
89.47 %
37
4.48 %
29
3.51 %
3
0.36 %
PAR-PERSIS
24
2,057
7.71 %
102
4.96 %
1894
92.08 %
80
3.89 %
60
2.92 %
1
0.05 %
PAR-RAPID
27
102
0.38 %
24
23.53 %
72
70.59 %
6
5.88 %
5
4.90 %
1
0.98 %
SER-FLEXI
2
43
0.16 %
2
4.65 %
40
93.02 %
2
4.65 %
1
2.33 %
0
0.00 %
Powys
PAR-HERD-S
3
190
0.71 %
0
0.00 %
190
100.00 %
1
0.53 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-OTHER
3
194
0.73 %
6
3.09 %
182
93.81 %
5
2.58 %
5
2.58 %
1
0.52 %
PAR-PERSIS
4
170
0.64 %
8
4.71 %
161
94.71 %
3
1.76 %
1
0.59 %
0
0.00 %
PAR-RAPID
6
18
0.07 %
2
11.11 %
16
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-PERSIS
2
2
0.01 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-OTHER
2
7
0.03 %
2
28.57 %
4
57.14 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-PERSIS
4
10
0.04 %
2
20.00 %
7
70.00 %
1
10.00 %
1
10.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
4
378
1.42 %
13
3.44 %
326
86.24 %
42
11.11 %
38
10.05 %
1
0.26 %
PAR-OTHER
1
17
0.06 %
2
11.76 %
15
88.24 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
6
14
0.05 %
1
7.14 %
13
92.86 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
245
0.92 %
14
5.71 %
211
86.12 %
22
8.98 %
20
8.16 %
0
0.00 %
Powys
PAR-NEW-IN
4
1,108
4.15 %
24
2.17 %
1032
93.14 %
57
5.14 %
49
4.42 %
3
0.27 %
PAR-PERSIS
1
3
0.01 %
0
0.00 %
3
100.00 %
1
33.33 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
109
0.41 %
1
0.92 %
104
95.41 %
5
4.59 %
4
3.67 %
0
0.00 %
PAR-NEW-IN
4
44
0.16 %
2
4.55 %
38
86.36 %
4
9.09 %
4
9.09 %
0
0.00 %
NE Wales
PAR-HERD-S
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-NEW-IN
6
697
2.61 %
19
2.73 %
637
91.39 %
49
7.03 %
38
5.45 %
3
0.43 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
160
7315
27.43 %
397
5.43 %
6585
90.02 %
389
5.32 %
320
4.37 %
13
0.18 %
Grand Total
Sum:
316
26668
1198
24168
1575
1283
19
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
SER-FLEXI
2
73
0.03 %
1
1.37 %
64
87.67 %
8
10.96 %
8
10.96 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
104
11,826
5.06 %
361
3.05 %
10796
91.29 %
788
6.66 %
569
4.81 %
100
0.85 %
PAR-CUL-P
7
1,802
0.77 %
46
2.55 %
xx-xxxx-xxxx
94.28 %
73
4.05 %
57
3.16 %
0
0.00 %
PAR-PERSIS
18
3,804
1.63 %
156
4.10 %
3516
92.43 %
186
4.89 %
131
3.44 %
1
0.03 %
SER-FLEXI
2
372
0.16 %
9
2.42 %
354
95.16 %
10
2.69 %
9
2.42 %
0
0.00 %
Devon
PAR-CUL-N
258
28,608
12.24 %
1509
5.27 %
25588
89.44 %
1758
6.15 %
1366
4.77 %
145
0.51 %
PAR-CUL-P
15
1,584
0.68 %
110
6.94 %
1384
87.37 %
112
7.07 %
88
5.56 %
2
0.13 %
PAR-NEW-IN
1
14
0.01 %
1
7.14 %
10
71.43 %
3
21.43 %
3
21.43 %
0
0.00 %
PAR-PERSIS
1
92
0.04 %
8
8.70 %
80
86.96 %
8
8.70 %
4
4.35 %
0
0.00 %
SER-FLEXI
6
312
0.13 %
7
2.24 %
288
92.31 %
14
4.49 %
11
3.53 %
6
1.92 %
Dorset
PAR-CUL-N
62
7,751
3.32 %
434
5.60 %
6861
88.52 %
398
5.13 %
305
3.93 %
151
1.95 %
PAR-CUL-P
12
3,059
1.31 %
264
8.63 %
2661
86.99 %
183
5.98 %
133
4.35 %
1
0.03 %
PAR-NEW-IN
2
167
0.07 %
5
2.99 %
156
93.41 %
11
6.59 %
6
3.59 %
0
0.00 %
PAR-PERSIS
2
19
0.01 %
0
0.00 %
16
84.21 %
3
15.79 %
3
15.79 %
0
0.00 %
SER-FLEXI
9
807
0.35 %
52
6.44 %
734
90.95 %
34
4.21 %
21
2.60 %
0
0.00 %
Gloucs
PAR-CUL-N
56
4,931
2.11 %
214
4.34 %
4363
88.48 %
449
9.11 %
350
7.10 %
4
0.08 %
PAR-NEW-IN
1
38
0.02 %
4
10.53 %
32
84.21 %
4
10.53 %
2
5.26 %
0
0.00 %
PAR-OTHER
2
63
0.03 %
1
1.59 %
55
87.30 %
8
12.70 %
7
11.11 %
0
0.00 %
PAR-PERSIS
2
242
0.10 %
14
5.79 %
220
90.91 %
10
4.13 %
8
3.31 %
0
0.00 %
Heref
PAR-CUL-N
18
2,820
1.21 %
102
3.62 %
2614
92.70 %
137
4.86 %
99
3.51 %
5
0.18 %
PAR-NEW-IN
9
654
0.28 %
23
3.52 %
586
89.60 %
59
9.02 %
44
6.73 %
1
0.15 %
PAR-OTHER
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
213
0.09 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-CUL-N
22
5,497
2.35 %
111
2.02 %
5161
93.89 %
315
5.73 %
223
4.06 %
2
0.04 %
PAR-PERSIS
7
1,015
0.43 %
60
5.91 %
899
88.57 %
65
6.40 %
54
5.32 %
2
0.20 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
3
389
0.17 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
108
11,352
4.86 %
437
3.85 %
10515
92.63 %
551
4.85 %
371
3.27 %
29
0.26 %
PAR-CUL-P
3
418
0.18 %
13
3.11 %
391
93.54 %
18
4.31 %
14
3.35 %
0
0.00 %
PAR-PERSIS
3
293
0.13 %
25
8.53 %
258
88.05 %
14
4.78 %
10
3.41 %
0
0.00 %
SER-FLEXI
5
202
0.09 %
7
3.47 %
191
94.55 %
4
1.98 %
4
1.98 %
0
0.00 %
Staffs
PAR-CUL-N
21
2,900
1.24 %
78
2.69 %
2527
87.14 %
157
5.41 %
139
4.79 %
156
5.38 %
PAR-NEW-IN
51
6,797
2.91 %
320
4.71 %
6229
91.64 %
322
4.74 %
247
3.63 %
1
0.01 %
Wiltshire
PAR-CUL-N
66
8,018
3.43 %
217
2.71 %
7371
91.93 %
544
6.78 %
410
5.11 %
20
0.25 %
PAR-CUL-P
7
1,241
0.53 %
69
5.56 %
1096
88.32 %
95
7.66 %
65
5.24 %
11
0.89 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
104
0.04 %
5
4.81 %
93
89.42 %
7
6.73 %
6
5.77 %
0
0.00 %
SER-FLEXI
2
45
0.02 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
24
2,641
1.13 %
75
2.84 %
2369
89.70 %
255
9.66 %
197
7.46 %
0
0.00 %
PAR-PERSIS
3
319
0.14 %
2
0.63 %
308
96.55 %
11
3.45 %
9
2.82 %
0
0.00 %
Bucks
PAR-NEW-IN
15
1,963
0.84 %
79
4.02 %
1724
87.82 %
196
9.98 %
160
8.15 %
0
0.00 %
Cheshire
PAR-CUL-N
6
868
0.37 %
14
1.61 %
837
96.43 %
28
3.23 %
17
1.96 %
0
0.00 %
PAR-NEW-IN
141
24,388
10.43 %
490
2.01 %
22862
93.74 %
1283
5.26 %
986
4.04 %
50
0.21 %
Derbyshire
PAR-NEW-IN
121
14,554
6.23 %
492
3.38 %
12460
85.61 %
1049
7.21 %
850
5.84 %
752
5.17 %
E Sussex
PAR-NEW-IN
27
1,970
0.84 %
63
3.20 %
1820
92.39 %
101
5.13 %
87
4.42 %
0
0.00 %
Hampshire
PAR-CUL-N
2
363
0.16 %
15
4.13 %
327
90.08 %
39
10.74 %
19
5.23 %
2
0.55 %
PAR-NEW-IN
32
3,771
1.61 %
99
2.63 %
3487
92.47 %
266
7.05 %
185
4.91 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
48
4,740
2.03 %
179
3.78 %
4316
91.05 %
307
6.48 %
227
4.79 %
18
0.38 %
Northants
PAR-NEW-IN
20
1,583
0.68 %
58
3.66 %
1455
91.91 %
80
5.05 %
69
4.36 %
1
0.06 %
Notts
PAR-NEW-IN
6
366
0.16 %
4
1.09 %
350
95.63 %
17
4.64 %
11
3.01 %
1
0.27 %
Oxon
PAR-NEW-IN
82
7,789
3.33 %
345
4.43 %
6801
87.32 %
491
6.30 %
355
4.56 %
288
3.70 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Warks
PAR-NEW-IN
64
5,649
2.42 %
153
2.71 %
5137
90.94 %
342
6.05 %
255
4.51 %
104
1.84 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
479
0.20 %
20
4.18 %
446
93.11 %
20
4.18 %
13
2.71 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
384
0.16 %
4
1.04 %
363
94.53 %
32
8.33 %
17
4.43 %
0
0.00 %
Cleveland
PAR-NEW-IN
1
50
0.02 %
2
4.00 %
48
96.00 %
1
2.00 %
0
0.00 %
0
0.00 %
Cumbria
PAR-NEW-IN
3
48
0.02 %
1
2.08 %
40
83.33 %
8
16.67 %
7
14.58 %
0
0.00 %
PAR-PERSIS
1
33
0.01 %
1
3.03 %
28
84.85 %
4
12.12 %
4
12.12 %
0
0.00 %
Essex
PAR-NEW-IN
3
265
0.11 %
3
1.13 %
252
95.09 %
11
4.15 %
10
3.77 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
7
336
0.14 %
6
1.79 %
114
33.93 %
6
1.79 %
6
1.79 %
210
62.50 %
Humberside
PAR-NEW-IN
2
78
0.03 %
7
8.97 %
66
84.62 %
7
8.97 %
5
6.41 %
0
0.00 %
Kent
PAR-NEW-IN
1
58
0.02 %
1
1.72 %
53
91.38 %
4
6.90 %
4
6.90 %
0
0.00 %
Lincs
PAR-NEW-IN
7
358
0.15 %
15
4.19 %
325
90.78 %
19
5.31 %
18
5.03 %
0
0.00 %
PAR-PERSIS
2
34
0.01 %
0
0.00 %
31
91.18 %
5
14.71 %
3
8.82 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
49
0.02 %
1
2.04 %
29
59.18 %
6
12.24 %
6
12.24 %
13
26.53 %
Northumb
PAR-NEW-IN
2
307
0.13 %
17
5.54 %
240
78.18 %
40
13.03 %
50
16.29 %
0
0.00 %
N Yorks
PAR-NEW-IN
10
849
0.36 %
16
1.88 %
713
83.98 %
40
4.71 %
29
3.42 %
91
10.72 %
Surrey
PAR-NEW-IN
3
429
0.18 %
17
3.96 %
396
92.31 %
18
4.20 %
16
3.73 %
0
0.00 %
W Yorks
PAR-NEW-IN
8
563
0.24 %
4
0.71 %
473
84.01 %
37
6.57 %
25
4.44 %
61
10.83 %
England
1546
182831
78.23 %
6875
3.76 %
165268
90.39 %
11130
6.09 %
8460
4.63 %
2228
1.22 %
Wales - High TB
Carms
PAR-HERD-S
12
678
0.29 %
51
7.52 %
598
88.20 %
45
6.64 %
29
4.28 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
42
809
0.35 %
123
15.20 %
649
80.22 %
44
5.44 %
37
4.57 %
0
0.00 %
PAR-PERSIS
56
3,052
1.31 %
230
7.54 %
2686
88.01 %
168
5.50 %
131
4.29 %
5
0.16 %
PAR-RAPID
73
281
0.12 %
54
19.22 %
209
74.38 %
26
9.25 %
18
6.41 %
0
0.00 %
SER-FLEXI
6
84
0.04 %
22
26.19 %
59
70.24 %
2
2.38 %
1
1.19 %
2
2.38 %
Ceredigion
PAR-HERD-S
2
13
0.01 %
0
0.00 %
7
53.85 %
6
46.15 %
6
46.15 %
0
0.00 %
PAR-OTHER
32
692
0.30 %
66
9.54 %
596
86.13 %
39
5.64 %
30
4.34 %
0
0.00 %
PAR-PERSIS
29
1,456
0.62 %
69
4.74 %
1310
89.97 %
84
5.77 %
75
5.15 %
2
0.14 %
PAR-RAPID
37
113
0.05 %
31
27.43 %
77
68.14 %
7
6.19 %
5
4.42 %
0
0.00 %
SER-FLEXI
2
40
0.02 %
0
0.00 %
38
95.00 %
2
5.00 %
2
5.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gwent
PAR-HERD-S
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
25
1,447
0.62 %
92
6.36 %
1311
90.60 %
68
4.70 %
43
2.97 %
1
0.07 %
PAR-RAPID
18
110
0.05 %
18
16.36 %
89
80.91 %
3
2.73 %
3
2.73 %
0
0.00 %
SER-FLEXI
9
284
0.12 %
27
9.51 %
251
88.38 %
10
3.52 %
6
2.11 %
0
0.00 %
Pembs
PAR-HERD-S
7
292
0.12 %
11
3.77 %
271
92.81 %
12
4.11 %
10
3.42 %
0
0.00 %
PAR-NEW-IN
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
110
2,493
1.07 %
268
10.75 %
2136
85.68 %
117
4.69 %
86
3.45 %
3
0.12 %
PAR-PERSIS
151
9,824
4.20 %
472
4.80 %
8946
91.06 %
493
5.02 %
395
4.02 %
11
0.11 %
PAR-RAPID
126
529
0.23 %
113
21.36 %
398
75.24 %
23
4.35 %
17
3.21 %
1
0.19 %
SER-FLEXI
22
467
0.20 %
17
3.64 %
436
93.36 %
24
5.14 %
14
3.00 %
0
0.00 %
Powys
PAR-HERD-S
15
262
0.11 %
9
3.44 %
253
96.56 %
3
1.15 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
2
115
0.05 %
6
5.22 %
107
93.04 %
2
1.74 %
2
1.74 %
0
0.00 %
PAR-OTHER
17
994
0.43 %
52
5.23 %
858
86.32 %
92
9.26 %
83
8.35 %
1
0.10 %
PAR-PERSIS
56
1,948
0.83 %
111
5.70 %
1677
86.09 %
178
9.14 %
151
7.75 %
9
0.46 %
PAR-RAPID
45
141
0.06 %
22
15.60 %
116
82.27 %
4
2.84 %
3
2.13 %
0
0.00 %
SER-FLEXI
10
212
0.09 %
17
8.02 %
186
87.74 %
19
8.96 %
9
4.25 %
0
0.00 %
S Wales
PAR-PERSIS
2
274
0.12 %
12
4.38 %
260
94.89 %
8
2.92 %
2
0.73 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
36
0.02 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-PERSIS
5
18
0.01 %
0
0.00 %
16
88.89 %
4
22.22 %
2
11.11 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
30
0.01 %
5
16.67 %
24
80.00 %
3
10.00 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
1
155
0.07 %
34
21.94 %
119
76.77 %
2
1.29 %
2
1.29 %
0
0.00 %
PAR-OTHER
11
231
0.10 %
20
8.66 %
201
87.01 %
12
5.19 %
10
4.33 %
0
0.00 %
PAR-PERSIS
12
577
0.25 %
34
5.89 %
526
91.16 %
20
3.47 %
17
2.95 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
7
11
0.00 %
6
54.55 %
4
36.36 %
1
9.09 %
1
9.09 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
171
0.07 %
12
7.02 %
152
88.89 %
8
4.68 %
7
4.09 %
0
0.00 %
PAR-RAPID
5
29
0.01 %
8
27.59 %
21
72.41 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
48
0.02 %
6
12.50 %
40
83.33 %
2
4.17 %
2
4.17 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
86
8,327
3.56 %
228
2.74 %
7670
92.11 %
516
6.20 %
426
5.12 %
3
0.04 %
PAR-OTHER
26
385
0.16 %
31
8.05 %
335
87.01 %
23
5.97 %
19
4.94 %
0
0.00 %
PAR-PERSIS
18
1,552
0.66 %
90
5.80 %
1410
90.85 %
63
4.06 %
49
3.16 %
3
0.19 %
PAR-RAPID
29
86
0.04 %
19
22.09 %
64
74.42 %
3
3.49 %
3
3.49 %
0
0.00 %
SER-FLEXI
11
430
0.18 %
21
4.88 %
380
88.37 %
37
8.60 %
27
6.28 %
2
0.47 %
Powys
PAR-NEW-IN
17
2,022
0.87 %
42
2.08 %
1881
93.03 %
114
5.64 %
95
4.70 %
4
0.20 %
PAR-OTHER
4
103
0.04 %
1
0.97 %
89
86.41 %
18
17.48 %
13
12.62 %
0
0.00 %
PAR-PERSIS
4
283
0.12 %
4
1.41 %
263
92.93 %
19
6.71 %
16
5.65 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
13
0.01 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
1
7
0.00 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
153
0.07 %
1
0.65 %
146
95.42 %
7
4.58 %
6
3.92 %
0
0.00 %
PAR-NEW-IN
32
1,739
0.74 %
33
1.90 %
1629
93.67 %
101
5.81 %
74
4.26 %
3
0.17 %
PAR-OTHER
3
9
0.00 %
0
0.00 %
5
55.56 %
4
44.44 %
4
44.44 %
0
0.00 %
PAR-RAPID
2
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
NE Wales
PAR-HERD-S
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-NEW-IN
26
3,207
1.37 %
61
1.90 %
2990
93.23 %
214
6.67 %
153
4.77 %
3
0.09 %
PAR-OTHER
3
14
0.01 %
1
7.14 %
11
78.57 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-PERSIS
1
40
0.02 %
0
0.00 %
32
80.00 %
10
25.00 %
8
20.00 %
0
0.00 %
PAR-RAPID
4
21
0.01 %
1
4.76 %
20
95.24 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
1246
46356
19.83 %
2565
5.53 %
41636
89.82 %
2669
5.76 %
2102
4.53 %
53
0.11 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
7
588
0.25 %
32
5.44 %
455
77.38 %
41
6.97 %
36
6.12 %
65
11.05 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
7
782
0.33 %
15
1.92 %
700
89.51 %
94
12.02 %
67
8.57 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
383
0.16 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Berwickshire
PAR-NEW-IN
2
78
0.03 %
8
10.26 %
62
79.49 %
8
10.26 %
8
10.26 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
6
849
0.36 %
12
1.41 %
802
94.46 %
38
4.48 %
35
4.12 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
15
807
0.35 %
22
2.73 %
762
94.42 %
34
4.21 %
23
2.85 %
0
0.00 %
PAR-PERSIS
1
22
0.01 %
0
0.00 %
22
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Perthshire
PAR-NEW-IN
2
357
0.15 %
0
0.00 %
345
96.64 %
14
3.92 %
12
3.36 %
0
0.00 %
Wigtown
PAR-NEW-IN
6
664
0.28 %
17
2.56 %
624
93.98 %
28
4.22 %
23
3.46 %
0
0.00 %
Scotland
49
4530
1.94 %
107
2.36 %
4131
91.19 %
287
6.34 %
227
5.01 %
65
1.43 %
Grand Total
Sum:
xx-xxxx-xxxx
9547
211035
14086
10789
2346
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol October 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
154
19348
100.0%
800
4.1%
17581
90.9%
1184
6.1%
961
5.0%
6
0.0%
Serial/Extended
2
5
0.0%
1
20.0%
2
40.0%
2
40.0%
2
40.0%
0
0.0 %
Total
156
19353
100.0%
801
4.1%
17583
90.9%
1186
6.1%
963
5.0%
6
0.0%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
155
6999
95.7%
378
5.4%
6309
90.1%
365
5.2%
299
4.3%
13
0.2%
Serial/Extended
5
316
4.3%
19
6.0%
276
87.3%
24
7.6%
21
6.6%
0
0.0 %
Total
160
7315
100.0%
397
5.4%
6585
90.0%
389
5.3%
320
4.4%
13
0.2%
GB
Parallel
309
26347
98.8%
1178
4.5%
23890
90.7%
1549
5.9%
1260
4.8%
19
0.1%
Serial/Extended
7
321
1.2%
20
6.2%
278
86.6%
26
8.1%
23
7.2%
0
0.0 %
Total
316
26668
100.0%
1198
4.5%
24168
90.6%
1575
5.9%
1283
4.8%
19
0.1%
156.00
160.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
98.8%
6779
3.8% 163225
90.4%
11033
6.1%
8386
4.6%
2222
1.2%
Serial/Extended
31
2219
1.2%
96
4.3%
2043
92.1%
97
4.4%
74
3.3%
6
0.3%
Total
xx-xxxx-xxxx
100.0%
6875
3.8% 165268
90.4%
11130
6.1%
8460
4.6%
2228
1.2%
Scotland
Parallel
49
4530
100.0%
107
2.4%
4131
91.2%
287
6.3%
227
5.0%
65
1.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
49
4530
100.0%
107
2.4%
4131
91.2%
287
6.3%
227
5.0%
65
1.4%
Wales
Parallel
1179
44733
96.5%
2449
5.5%
40194
89.9%
2573
5.8%
2041
4.6%
49
0.1%
Serial/Extended
67
1623
3.5%
116
7.1%
1442
88.8%
96
5.9%
61
3.8%
4
0.2%
Total
1246
46356
100.0%
2565
5.5%
41636
89.8%
2669
5.8%
2102
4.5%
53
0.1%
GB
Parallel
xx-xxxx-xxxx
98.4%
9335
4.1% 207550
90.3%
13893
6.0%
10654
4.6%
2336
1.0%
Serial/Extended
98
3842
1.6%
212
5.5%
3485
90.7%
193
5.0%
135
3.5%
10
0.3%
Total
xx-xxxx-xxxx
100.0%
9547
4.1% 211035
90.3%
14086
6.0%
10789
4.6%
2346
1.0%
#######
#######
49.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
7
321
1.2%
20
6.2%
278
86.6%
26
8.1 %
23
7.2 %
0
Parallel - Herd or Group Slaughter
5
301
1.1%
1
0.3%
295
98.0%
7
2.3 %
5
1.7 %
0
Parallel New Infection
78
11248
42.2%
285
2.5%
10376
92.2%
707
6.3 %
580
5.2 %
7
0.1 %
Parallel - new infection cull area
87
9096
34.1%
495
5.4%
8139
89.5%
567
6.2 %
456
5.0 %
6
0.1 %
Parallel - Other
28
1406
5.3%
121
8.6%
1230
87.5%
61
4.3 %
51
3.6 %
4
0.3 %
Parallel Persistent Infection
53
3371
12.6%
180
5.3%
3064
90.9%
157
4.7 %
126
3.7 %
1
0.0 %
Parallel - persistent infection cull area
6
743
2.8%
57
7.7%
651
87.6%
42
5.7 %
35
4.7 %
0
Parallel - Rapid Testing Two-times IRs
52
182
0.7%
39
21.4%
135
74.2%
8
4.4 %
7
3.8 %
1
0.5 %
Total
316
26668
100.0%
1198
4.5%
24168
90.6%
1575
5.9 %
1283
4.8 %
19
0.1 %
Table 4a. Summaryof IFN Gamma results by submission reason (October 2020)
England - HRA
Extended Flexible IFN-GAMMA test
2
5
0.0%
1
20.0%
2
40.0%
2
40.0 %
2
40.0 %
0
Parallel New Infection
2
373
1.4%
10
2.7%
342
91.7%
22
5.9 %
21
5.6 %
0
Parallel - new infection cull area
87
9096
34.1%
495
5.4%
8139
89.5%
567
6.2 %
456
5.0 %
6
0.1 %
Parallel Persistent Infection
3
491
1.8%
21
4.3%
453
92.3%
18
3.7 %
17
3.5 %
0
Parallel - persistent infection cull area
6
743
2.8%
57
7.7%
651
87.6%
42
5.7 %
35
4.7 %
0
England - HRA
100
10708
40.2%
584
5.5%
9587
89.5%
651
6.1 %
531
5.0 %
6
0.1 %
England - Edge
Parallel New Infection
49
8277
31.0%
212
2.6%
7655
92.5%
512
6.2 %
410
5.0 %
0
Parallel Persistent Infection
1
4
0.0%
0
0.0 %
1
25.0%
3
75.0 %
3
75.0 %
0
England - Edge
50
8281
31.1%
212
2.6%
7656
92.5%
515
6.2 %
413
5.0 %
0
England - LRA
Parallel New Infection
6
364
1.4%
5
1.4%
340
93.4%
20
5.5 %
19
5.2 %
0
England - LRA
6
364
1.4%
5
1.4%
340
93.4%
20
5.5 %
19
5.2 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
3
71
0.3%
5
7.0%
65
91.5%
2
2.8 %
1
1.4 %
0
Parallel - Herd or Group Slaughter
3
190
0.7%
0
0.0 %
190
100.0%
1
0.5 %
0
0
Parallel New Infection
3
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel - Other
25
1382
5.2%
117
8.5%
1211
87.6%
60
4.3 %
50
3.6 %
4
0.3 %
Parallel Persistent Infection
44
2863
10.7%
157
5.5%
2600
90.8%
134
4.7 %
105
3.7 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
45
167
0.6%
38
22.8%
121
72.5%
8
4.8 %
7
4.2 %
1
0.6 %
Wales - High TB
123
4680
17.5%
317
6.8%
4193
89.6%
206
4.4 %
164
3.5 %
6
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
245
0.9%
14
5.7%
211
86.1%
22
9.0 %
20
8.2 %
0
Parallel New Infection
8
1486
5.6%
37
2.5%
1358
91.4%
99
6.7 %
87
5.9 %
4
0.3 %
Parallel - Other
3
24
0.1%
4
16.7%
19
79.2%
1
4.2 %
1
4.2 %
0
Parallel Persistent Infection
5
13
0.0%
2
15.4%
10
76.9%
2
15.4 %
1
7.7 %
0
Parallel - Rapid Testing Two-times IRs
6
14
0.1%
1
7.1%
13
92.9%
0
0
0
Wales - Intermediate TB
24
1782
6.7%
58
3.3%
1611
90.4%
124
7.0 %
109
6.1 %
4
0.2 %
Wales - Low TB
Parallel - Herd or Group Slaughter
2
111
0.4%
1
0.9%
105
94.6%
6
5.4 %
5
4.5 %
0
Parallel New Infection
10
741
2.8%
21
2.8%
675
91.1%
53
7.2 %
42
5.7 %
3
0.4 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
13
853
3.2%
22
2.6%
781
91.6%
59
6.9 %
47
5.5 %
3
0.4 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
96
3823
1.6%
212
5.5%
3466
90.7%
193
5.0 %
135
3.5 %
10
0.3 %
Parallel - Herd or Group Slaughter
44
1433
0.6%
78
5.4%
1302
90.9%
77
5.4 %
53
3.7 %
0
Parallel New Infection
906 100155
42.9%
2980
3.0%
90717
90.6%
6157
6.1 %
4789
4.8 %
1669
1.7 %
Parallel - new infection cull area
723
84934
36.3%
3492
4.1%
76960
90.6%
5164
6.1 %
3868
4.6 %
614
0.7 %
Parallel - Other
260
6583
2.8%
579
8.8%
5639
85.7%
456
6.9 %
361
5.5 %
4
0.1 %
Parallel Persistent Infection
410
27312
11.7%
1426
5.2%
24679
90.4%
1488
5.4 %
1173
4.3 %
34
0.1 %
Parallel - persistent infection cull area
44
8104
3.5%
502
6.2%
7231
89.2%
481
5.9 %
357
4.4 %
14
0.2 %
Parallel - Rapid Testing Two-times IRs
356
1354
0.6%
278
20.5%
1022
75.5%
70
5.2 %
53
3.9 %
1
0.1 %
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
9547
4.1%
211035
90.3%
14086
6.0 %
10789
4.6 %
2346
1.0 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
29
2200
0.9%
96
4.4%
2024
92.0%
97
4.4 %
74
3.4 %
6
0.3 %
Parallel New Infection
65
7671
3.3%
353
4.6%
7014
91.4%
399
5.2 %
302
3.9 %
2
0.0 %
Parallel - new infection cull area
715
83703
35.8%
3463
4.1%
75796
90.6%
5097
6.1 %
3832
4.6 %
612
0.7 %
Parallel - Other
3
67
0.0%
1
1.5%
59
88.1%
8
11.9 %
7
10.4 %
0
Parallel Persistent Infection
37
5782
2.5%
277
4.8%
5254
90.9%
325
5.6 %
248
4.3 %
3
0.1 %
Parallel - persistent infection cull area
44
8104
3.5%
502
6.2%
7231
89.2%
481
5.9 %
357
4.4 %
14
0.2 %
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
England - Edge
Parallel New Infection
580
69414
29.7%
2037
2.9%
62781
90.4%
4387
6.3 %
3382
4.9 %
1214
1.7 %
Parallel - new infection cull area
8
1231
0.5%
29
2.4%
1164
94.6%
67
5.4 %
36
2.9 %
2
0.2 %
Parallel Persistent Infection
6
799
0.3%
22
2.8%
755
94.5%
31
3.9 %
22
2.8 %
0
England - LRA
Parallel New Infection
54
3774
1.6%
94
2.5%
3112
82.5%
229
6.1 %
193
5.1 %
375
9.9 %
Parallel Persistent Infection
3
67
0.0%
1
1.5%
59
88.1%
9
13.4 %
7
10.4 %
0
England
xx-xxxx-xxxx
78.2%
6875
3.8%
165268
90.4%
11130
6.1 %
8460
4.6 %
2228
1.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
51
1132
0.5%
84
7.4%
1014
89.6%
57
5.0 %
32
2.8 %
2
0.2 %
Parallel - Herd or Group Slaughter
37
1248
0.5%
72
5.8%
1131
90.6%
66
5.3 %
45
3.6 %
0
Parallel New Infection
4
120
0.1%
6
5.0%
112
93.3%
2
1.7 %
2
1.7 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2020) Cont.
Parallel - Other
203
4992
2.1%
510
10.2%
4239
84.9%
295
5.9 %
239
4.8 %
4
0.1 %
Parallel Persistent Infection
324
18019
7.7%
986
5.5%
16206
89.9%
1003
5.6 %
799
4.4 %
28
0.2 %
Parallel - Rapid Testing Two-times IRs
303
1182
0.5%
241
20.4%
894
75.6%
63
5.3 %
46
3.9 %
1
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
16
491
0.2%
32
6.5%
428
87.2%
39
7.9 %
29
5.9 %
2
0.4 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
104
10504
4.5%
304
2.9%
9670
92.1%
632
6.0 %
523
5.0 %
7
0.1 %
Parallel - Other
41
719
0.3%
52
7.2%
625
86.9%
53
7.4 %
42
5.8 %
0
Parallel Persistent Infection
38
2583
1.1%
140
5.4%
2351
91.0%
110
4.3 %
89
3.4 %
3
0.1 %
Parallel - Rapid Testing Two-times IRs
47
139
0.1%
36
25.9%
99
71.2%
4
2.9 %
4
2.9 %
0
Wales - Low TB
Parallel - Herd or Group Slaughter
4
155
0.1%
1
0.6%
147
94.8%
8
5.2 %
7
4.5 %
0
Parallel New Infection
58
4946
2.1%
94
1.9%
4619
93.4%
315
6.4 %
227
4.6 %
6
0.1 %
Parallel - Other
6
23
0.0%
1
4.3%
16
69.6%
6
26.1 %
6
26.1 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
6
33
0.0%
1
3.0%
29
87.9%
3
9.1 %
3
9.1 %
0
Wales
1246
46356
19.8%
2565
5.5%
41636
89.8%
2669
5.8 %
2102
4.5 %
53
0.1 %
Scotland - Scotland
Parallel New Infection
41
3726
1.6%
92
2.5%
3409
91.5%
193
5.2 %
160
4.3 %
65
1.7 %
Parallel - Other
7
782
0.3%
15
1.9%
700
89.5%
94
12.0 %
67
8.6 %
0
Parallel Persistent Infection
1
22
0.0%
0
0.0 %
22
100.0%
0
0
0
Scotland
49
4530
1.9%
107
2.4%
4131
91.2%
287
6.3 %
227
5.0 %
65
1.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 November
272
30186
169
1080
14
0
0
0
0
72
5
0
15
0
910
0
61
0
1
December
234
25192
138
904
2
0
0
0
0
0
87
2
0
4
0
759
0
49
0
1
2020 January
343
31274
227
1367
1
7
0
0
0
0
178
3
0
8
0
1110
1
57
0
0
February
323
33146
192
1289
1
21
0
0
0
0
187
3
0
6
0
982
0
89
0
0
March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
2
66
0
3
August
290
24676
173
888
20
0
0
0
0
145
0
1
3
0
639
29
50
0
1
September
320
25394
175
762
7
0
0
0
0
68
0
5
0
1
615
64
0
0
2
October
316
26668
189
1198
303
607
0
0
0
0
5
0
1
0
0
75
5
0
0
0
Totals (last
12 months)
3347
289095
1962
11531
311
730
0
0
0
0
1218
19
7
59
1
8307
102
561
0
9
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1853
225496
1170
8269
597
0
0
0
0
1111
9
2
29
0
6182
11
38
0
3
Scotland
50
4535
24
107
1
0
0
0
0
13
0
0
5
0
71
0
17
0
0
Wales
1444
59064
768
3155
132
0
0
0
0
94
10
5
25
1
2054
91
506
0
6
Totals
3347
289095
1962
11531
730
0
0
0
0
1218
19
7
59
1
8307
102
561
0
9
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
160
Wiltshire
Warks
Somerset excl N
Powys
Oxon
NE Wales
Leics & Rut
Heref
Gwynedd
Gloucs
Essex
Devon
Cumbria
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during October 2020 (% submission per
County)
County
%Resampled %Retested
Berks
75.00 %
75.00 %
Bucks
17.47 %
19.70 %
Carms
10.53 %
10.53 %
Ceredigion
6.27 %
6.61 %
Cheshire
3.23 %
4.36 %
Corn & Scilly
4.98 %
5.92 %
Cumbria
21.43 %
21.43 %
Derbyshire
8.17 %
9.01 %
Devon
4.78 %
5.70 %
Dorset
4.75 %
5.56 %
Essex
3.77 %
4.15 %
E Sussex
3.95 %
5.26 %
Gloucs
6.29 %
8.14 %
Gwent
3.90 %
5.45 %
Gwynedd
5.23 %
5.88 %
Hampshire
5.67 %
5.67 %
Heref
5.45 %
6.41 %
Kent
6.90 %
6.90 %
Leics & Rut
6.03 %
7.00 %
Lincs
7.41 %
7.41 %
NE Wales
7.16 %
8.42 %
Northants
2.88 %
3.10 %
Oxon
3.95 %
6.91 %
Pembs
3.13 %
4.12 %
Powys
3.32 %
4.04 %
Shropshire
4.79 %
6.46 %
Somerset excl
4.18 %
5.82 %
Staffs
7.34 %
7.74 %
Warks
4.13 %
6.46 %
W Glamorgan
50.00 %
Wiltshire
2.96 %
2.96 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19191
801
4.2%
19353
801
4.1%
Wales
7243
397
5.5%
7315
397
5.4%
GB
26434
1198
4.5%
26668
1198
4.5%
Table 6a (i): Number of animals and submissions tested in October 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
173495
6875
4.0%
182831
6875
3.8%
Wales
42989
2564
6.0%
46356
2565
5.5%
Scotland
3635
107
2.9%
4530
107
2.4%
GB
220118
9546
4.3%
233717
9547
4.1%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19686
483
2.5%
20315
483
2.4%
Wales
5803
253
4.4%
5904
253
4.3%
Scotland
447
7
1.6%
471
7
1.5%
GB
25936
743
2.9%
26690
743
2.8%
Table 6a (ii): Number of animals and submissions tested in October 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
195986
8632
4.4%
221109
8632
3.9%
Wales
48299
2608
5.4%
52791
2608
4.9%
Scotland
2010
90
4.5%
2688
90
3.3%
GB
246291
11330
4.6%
276588
11330
4.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cheshire
PRV-SE
1
12
1
8.33 %
PRV-SP
2
2
0
0
Dorset
PRV-SP
1
1
0
0
Northern Somerset & South Glou
PRV-SE
1
39
1
2.56 %
Wiltshire
PRV-SE
2
280
15
5.36 %
Total
7
334
17
5.09 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples October 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SE
1
12
1
8.33 %
PRV-SP
3
3
0
0
Cumbria
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
3
5
0
0
East Sussex
PRV-SP
1
1
0
0
Essex
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Hampshire
PRV-SP
1
3
0
0
Northern Somerset & South Glou
PRV-SE
1
39
1
2.56 %
Oxfordshire
PRV-SP
1
32
0
0
Somerset excl North
PRV-SE
1
28
0
0
PRV-SP
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Wiltshire
PRV-SE
2
280
15
5.36 %
Worcestershire
PRV-SP
1
1
0
0
Total
24
569
20
3.51 %
Isle of Man
SER-IOM
15
173
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Berkshire
1
263
7
2.7%
Carmarthenshire
8
120
17
14.2%
Ceredigion
5
36
3
8.3%
Devon
2
466
29
6.2%
North East Wales
9
307
23
7.5%
Pembrokeshire
18
334
39
11.7%
Powys
1
4
1
25.0%
South Wales
5
41
6
14.6%
Wiltshire
1
290
5
1.7%
TC0077
50
1,861
130
7.0 %
Table 8a Number of Cattle Antibody Tests October 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Berkshire
2
315
11
3.5%
Carmarthenshire
47
739
130
17.6%
Ceredigion
33
392
63
16.1%
Devon
6
1,498
124
8.3%
North East Wales
43
1,237
119
9.6%
Pembrokeshire
117
1,919
275
14.3%
Powys
32
434
70
16.1%
South Wales
26
379
68
17.9%
Staffordshire
1
22
1
4.5%
Wiltshire
2
581
8
1.4%
TC0077
309
7,516
869
11.6 %
Table 8b Number of Cattle Antibody Tests 2020