Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - January 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 161
Report Period 1st - 31st January 2020
Issued 20 February 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
January
2020
%
Total 2020
%
Submissions
343
343
Samples
Total (%)
31274
100.00 %
31274
100.00 %
England
24247
77.53 %
24247
77.53 %
Wales
5757
18.41 %
5757
18.41 %
Scotland
1270
4.06 %
1270
4.06 %
Parallel Tests
PAR-CUL-N
8782
28.08 %
8782
28.08 %
PAR-CUL-P
2044
6.54 %
2044
6.54 %
PAR-HERD-S
22
0.07 %
22
0.07 %
PAR-NEW-IN
13601
43.49 %
13601
43.49 %
PAR-OTHER
596
1.91 %
596
1.91 %
PAR-PERSIS
5068
16.21 %
5068
16.21 %
PAR-RAPID
61
0.20 %
61
0.20 %
Total(% of all samples)
30174
96.48 %
30174
96.48 %
Serial/Extended Tests
SER-FLEXI
1100
3.52 %
1100
3.52 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
Total(% of all samples)
1100
3.52 %
1100
3.52 %
Total
31274
100.00 %
31274
100.00 %
Retests
Total (% of all samples)
2068
6.61 %
2068
6.61 %
England
1546
4.94 %
1546
4.94 %
Wales
448
1.43 %
448
1.43 %
Scotland
74
0.24 %
74
0.24 %
Resamples
Total (% of all samples)
1611
5.15 %
1611
5.15 %
England
1174
3.75 %
1174
3.75 %
Wales
375
1.20 %
375
1.20 %
Scotland
62
0.20 %
62
0.20 %
Rejects
Total (% of all samples)
497
1.59 %
497
1.59 %
England
487
1.56 %
487
1.56 %
Wales
10
0.03 %
10
0.03 %
Scotland
0
0.00 %
Table 1. Monthly Totals for January 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
8782
28.1 %
PAR-CUL-P
2044
6.5 %
PAR-HERD-S
22
0.1 %
PAR-NEW-IN
13601
43.5 %
PAR-OTHER
596
1.9 %
PAR-PERSIS
5068
16.2 %
PAR-RAPID
61
0.2 %
SER-FLEXI
1100
3.5 %
Total
31274
100.00 %
Figure 1. Number of samples submitted in each category during January 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during January 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
1000
2000
3000
4000
5000
6000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
5
764
2.44 %
26
3.40 %
698
91.36 %
45
5.89 %
40
5.24 %
0
0.00 %
PAR-CUL-P
2
351
1.12 %
22
6.27 %
314
89.46 %
17
4.84 %
15
4.27 %
0
0.00 %
PAR-PERSIS
8
1,941
6.21 %
98
5.05 %
1792
92.32 %
88
4.53 %
51
2.63 %
0
0.00 %
Devon
PAR-CUL-N
38
5,062
16.19 %
186
3.67 %
4497
88.84 %
333
6.58 %
278
5.49 %
101
2.00 %
PAR-CUL-P
6
723
2.31 %
34
4.70 %
630
87.14 %
72
9.96 %
59
8.16 %
0
0.00 %
SER-FLEXI
2
48
0.15 %
2
4.17 %
44
91.67 %
2
4.17 %
2
4.17 %
0
0.00 %
Dorset
PAR-CUL-N
8
637
2.04 %
94
14.76 %
514
80.69 %
40
6.28 %
29
4.55 %
0
0.00 %
PAR-CUL-P
1
132
0.42 %
6
4.55 %
124
93.94 %
5
3.79 %
2
1.52 %
0
0.00 %
SER-FLEXI
2
391
1.25 %
29
7.42 %
351
89.77 %
17
4.35 %
11
2.81 %
0
0.00 %
Gloucs
PAR-CUL-N
6
433
1.38 %
25
5.77 %
375
86.61 %
42
9.70 %
33
7.62 %
0
0.00 %
Heref
PAR-CUL-N
4
552
1.77 %
22
3.99 %
515
93.30 %
19
3.44 %
14
2.54 %
1
0.18 %
PAR-NEW-IN
2
244
0.78 %
10
4.10 %
204
83.61 %
37
15.16 %
29
11.89 %
1
0.41 %
PAR-PERSIS
2
213
0.68 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-PERSIS
3
215
0.69 %
10
4.65 %
192
89.30 %
13
6.05 %
11
5.12 %
2
0.93 %
SER-FLEXI
3
389
1.24 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
14
582
1.86 %
23
3.95 %
503
86.43 %
39
6.70 %
28
4.81 %
28
4.81 %
PAR-CUL-P
1
261
0.83 %
13
4.98 %
238
91.19 %
13
4.98 %
10
3.83 %
0
0.00 %
Wiltshire
PAR-CUL-N
8
370
1.18 %
10
2.70 %
319
86.22 %
42
11.35 %
36
9.73 %
5
1.35 %
PAR-CUL-P
3
577
1.84 %
35
6.07 %
523
90.64 %
32
5.55 %
19
3.29 %
0
0.00 %
PAR-PERSIS
1
97
0.31 %
5
5.15 %
88
90.72 %
5
5.15 %
4
4.12 %
0
0.00 %
SER-FLEXI
1
26
0.08 %
3
11.54 %
23
88.46 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
5
97
0.31 %
4
4.12 %
87
89.69 %
14
14.43 %
6
6.19 %
0
0.00 %
Bucks
PAR-NEW-IN
6
296
0.95 %
6
2.03 %
237
80.07 %
55
18.58 %
53
17.91 %
0
0.00 %
Cheshire
PAR-NEW-IN
23
3,640
11.64 %
98
2.69 %
3427
94.15 %
166
4.56 %
115
3.16 %
0
0.00 %
Derbyshire
PAR-NEW-IN
17
1,857
5.94 %
68
3.66 %
1599
86.11 %
120
6.46 %
89
4.79 %
101
5.44 %
E Sussex
PAR-NEW-IN
5
452
1.45 %
22
4.87 %
419
92.70 %
15
3.32 %
11
2.43 %
0
0.00 %
Table 2a. Summary by County for January 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-CUL-N
1
361
1.15 %
15
4.16 %
327
90.58 %
39
10.80 %
19
5.26 %
0
0.00 %
PAR-NEW-IN
2
260
0.83 %
4
1.54 %
236
90.77 %
24
9.23 %
20
7.69 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
7
592
1.89 %
21
3.55 %
544
91.89 %
34
5.74 %
27
4.56 %
0
0.00 %
Northants
PAR-NEW-IN
5
298
0.95 %
8
2.68 %
272
91.28 %
22
7.38 %
18
6.04 %
0
0.00 %
Notts
PAR-NEW-IN
1
75
0.24 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Oxon
PAR-CUL-N
1
21
0.07 %
3
14.29 %
14
66.67 %
4
19.05 %
4
19.05 %
0
0.00 %
PAR-NEW-IN
13
1,426
4.56 %
60
4.21 %
1200
84.15 %
97
6.80 %
66
4.63 %
100
7.01 %
Warks
PAR-NEW-IN
6
657
2.10 %
17
2.59 %
582
88.58 %
26
3.96 %
14
2.13 %
44
6.70 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
26
0.08 %
1
3.85 %
24
92.31 %
1
3.85 %
1
3.85 %
0
0.00 %
Norfolk
PAR-NEW-IN
2
40
0.13 %
1
2.50 %
20
50.00 %
6
15.00 %
6
15.00 %
13
32.50 %
N Yorks
PAR-NEW-IN
1
141
0.45 %
1
0.71 %
49
34.75 %
1
0.71 %
0
0.00 %
91
64.54 %
England
216
24247
77.53 %
1009
4.16 %
21577
88.99 %
1546
6.38 %
1174
4.84 %
487
2.01 %
Wales - High TB
Carms
PAR-HERD-S
1
7
0.02 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
47
0.15 %
7
14.89 %
39
82.98 %
1
2.13 %
1
2.13 %
0
0.00 %
PAR-PERSIS
7
583
1.86 %
71
12.18 %
496
85.08 %
23
3.95 %
16
2.74 %
0
0.00 %
PAR-RAPID
7
13
0.04 %
4
30.77 %
8
61.54 %
1
7.69 %
1
7.69 %
0
0.00 %
Ceredigion
PAR-OTHER
1
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-PERSIS
6
392
1.25 %
13
3.32 %
362
92.35 %
19
4.85 %
15
3.83 %
2
0.51 %
PAR-RAPID
3
10
0.03 %
3
30.00 %
7
70.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
5
309
0.99 %
8
2.59 %
295
95.47 %
11
3.56 %
6
1.94 %
0
0.00 %
PAR-RAPID
3
7
0.02 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
87
0.28 %
5
5.75 %
79
90.80 %
3
3.45 %
3
3.45 %
0
0.00 %
Pembs
PAR-HERD-S
2
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-OTHER
8
540
1.73 %
42
7.78 %
480
88.89 %
26
4.81 %
18
3.33 %
0
0.00 %
PAR-PERSIS
11
946
3.02 %
62
6.55 %
802
84.78 %
88
9.30 %
79
8.35 %
3
0.32 %
PAR-RAPID
7
27
0.09 %
9
33.33 %
17
62.96 %
1
3.70 %
1
3.70 %
0
0.00 %
SER-FLEXI
2
35
0.11 %
0
0.00 %
33
94.29 %
4
11.43 %
2
5.71 %
0
0.00 %
Powys
PAR-HERD-S
3
10
0.03 %
1
10.00 %
9
90.00 %
2
20.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
7
873
2.79 %
30
3.44 %
735
84.19 %
113
12.94 %
103
11.80 %
5
0.57 %
PAR-PERSIS
8
114
0.36 %
15
13.16 %
54
47.37 %
48
42.11 %
45
39.47 %
0
0.00 %
SER-FLEXI
4
84
0.27 %
2
2.38 %
78
92.86 %
5
5.95 %
4
4.76 %
0
0.00 %
S Wales
PAR-PERSIS
1
69
0.22 %
3
4.35 %
64
92.75 %
6
8.70 %
2
2.90 %
0
0.00 %
SER-FLEXI
1
36
0.12 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.01 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
48
0.15 %
1
2.08 %
47
97.92 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
6
0.02 %
3
50.00 %
2
33.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
9
894
2.86 %
20
2.24 %
820
91.72 %
61
6.82 %
54
6.04 %
0
0.00 %
PAR-PERSIS
3
135
0.43 %
27
20.00 %
103
76.30 %
5
3.70 %
5
3.70 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
2
92
0.29 %
1
1.09 %
89
96.74 %
5
5.43 %
2
2.17 %
0
0.00 %
S Glamorgan
SER-FLEXI
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
1
35
0.11 %
2
5.71 %
29
82.86 %
4
11.43 %
4
11.43 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
1
336
1.07 %
1
0.30 %
327
97.32 %
16
4.76 %
8
2.38 %
0
0.00 %
Wales
117
5757
18.41 %
335
5.82 %
5037
87.49 %
448
7.78 %
375
6.51 %
10
0.17 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
383
1.22 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
3
569
1.82 %
8
1.41 %
538
94.55 %
26
4.57 %
23
4.04 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
1
21
0.07 %
0
0.00 %
21
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
297
0.95 %
14
4.71 %
267
89.90 %
18
6.06 %
16
5.39 %
0
0.00 %
Scotland
10
1270
4.06 %
23
1.81 %
1185
93.31 %
74
5.83 %
62
4.88 %
0
0.00 %
Grand Total
Sum:
343
31274
1367
27799
2068
1611
497
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
5
764
2.44 %
26
3.40 %
698
91.36 %
45
5.89 %
40
5.24 %
0
0.00 %
PAR-CUL-P
2
351
1.12 %
22
6.27 %
314
89.46 %
17
4.84 %
15
4.27 %
0
0.00 %
PAR-PERSIS
8
1,941
6.21 %
98
5.05 %
1792
92.32 %
88
4.53 %
51
2.63 %
0
0.00 %
Devon
PAR-CUL-N
38
5,062
16.19 %
186
3.67 %
4497
88.84 %
333
6.58 %
278
5.49 %
101
2.00 %
PAR-CUL-P
6
723
2.31 %
34
4.70 %
630
87.14 %
72
9.96 %
59
8.16 %
0
0.00 %
SER-FLEXI
2
48
0.15 %
2
4.17 %
44
91.67 %
2
4.17 %
2
4.17 %
0
0.00 %
Dorset
PAR-CUL-N
8
637
2.04 %
94
14.76 %
514
80.69 %
40
6.28 %
29
4.55 %
0
0.00 %
PAR-CUL-P
1
132
0.42 %
6
4.55 %
124
93.94 %
5
3.79 %
2
1.52 %
0
0.00 %
SER-FLEXI
2
391
1.25 %
29
7.42 %
351
89.77 %
17
4.35 %
11
2.81 %
0
0.00 %
Gloucs
PAR-CUL-N
6
433
1.38 %
25
5.77 %
375
86.61 %
42
9.70 %
33
7.62 %
0
0.00 %
Heref
PAR-CUL-N
4
552
1.77 %
22
3.99 %
515
93.30 %
19
3.44 %
14
2.54 %
1
0.18 %
PAR-NEW-IN
2
244
0.78 %
10
4.10 %
204
83.61 %
37
15.16 %
29
11.89 %
1
0.41 %
PAR-PERSIS
2
213
0.68 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-PERSIS
3
215
0.69 %
10
4.65 %
192
89.30 %
13
6.05 %
11
5.12 %
2
0.93 %
SER-FLEXI
3
389
1.24 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
14
582
1.86 %
23
3.95 %
503
86.43 %
39
6.70 %
28
4.81 %
28
4.81 %
PAR-CUL-P
1
261
0.83 %
13
4.98 %
238
91.19 %
13
4.98 %
10
3.83 %
0
0.00 %
Wiltshire
PAR-CUL-N
8
370
1.18 %
10
2.70 %
319
86.22 %
42
11.35 %
36
9.73 %
5
1.35 %
PAR-CUL-P
3
577
1.84 %
35
6.07 %
523
90.64 %
32
5.55 %
19
3.29 %
0
0.00 %
PAR-PERSIS
1
97
0.31 %
5
5.15 %
88
90.72 %
5
5.15 %
4
4.12 %
0
0.00 %
SER-FLEXI
1
26
0.08 %
3
11.54 %
23
88.46 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
5
97
0.31 %
4
4.12 %
87
89.69 %
14
14.43 %
6
6.19 %
0
0.00 %
Bucks
PAR-NEW-IN
6
296
0.95 %
6
2.03 %
237
80.07 %
55
18.58 %
53
17.91 %
0
0.00 %
Cheshire
PAR-NEW-IN
23
3,640
11.64 %
98
2.69 %
3427
94.15 %
166
4.56 %
115
3.16 %
0
0.00 %
Derbyshire
PAR-NEW-IN
17
1,857
5.94 %
68
3.66 %
1599
86.11 %
120
6.46 %
89
4.79 %
101
5.44 %
E Sussex
PAR-NEW-IN
5
452
1.45 %
22
4.87 %
419
92.70 %
15
3.32 %
11
2.43 %
0
0.00 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Hampshire
PAR-CUL-N
1
361
1.15 %
15
4.16 %
327
90.58 %
39
10.80 %
19
5.26 %
0
0.00 %
PAR-NEW-IN
2
260
0.83 %
4
1.54 %
236
90.77 %
24
9.23 %
20
7.69 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
7
592
1.89 %
21
3.55 %
544
91.89 %
34
5.74 %
27
4.56 %
0
0.00 %
Northants
PAR-NEW-IN
5
298
0.95 %
8
2.68 %
272
91.28 %
22
7.38 %
18
6.04 %
0
0.00 %
Notts
PAR-NEW-IN
1
75
0.24 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Oxon
PAR-CUL-N
1
21
0.07 %
3
14.29 %
14
66.67 %
4
19.05 %
4
19.05 %
0
0.00 %
PAR-NEW-IN
13
1,426
4.56 %
60
4.21 %
1200
84.15 %
97
6.80 %
66
4.63 %
100
7.01 %
Warks
PAR-NEW-IN
6
657
2.10 %
17
2.59 %
582
88.58 %
26
3.96 %
14
2.13 %
44
6.70 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
26
0.08 %
1
3.85 %
24
92.31 %
1
3.85 %
1
3.85 %
0
0.00 %
Norfolk
PAR-NEW-IN
2
40
0.13 %
1
2.50 %
20
50.00 %
6
15.00 %
6
15.00 %
13
32.50 %
N Yorks
PAR-NEW-IN
1
141
0.45 %
1
0.71 %
49
34.75 %
1
0.71 %
0
0.00 %
91
64.54 %
England
216
24247
77.53 %
1009
4.16 %
21577
88.99 %
1546
6.38 %
1174
4.84 %
487
2.01 %
Wales - High TB
Carms
PAR-HERD-S
1
7
0.02 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
47
0.15 %
7
14.89 %
39
82.98 %
1
2.13 %
1
2.13 %
0
0.00 %
PAR-PERSIS
7
583
1.86 %
71
12.18 %
496
85.08 %
23
3.95 %
16
2.74 %
0
0.00 %
PAR-RAPID
7
13
0.04 %
4
30.77 %
8
61.54 %
1
7.69 %
1
7.69 %
0
0.00 %
Ceredigion
PAR-OTHER
1
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-PERSIS
6
392
1.25 %
13
3.32 %
362
92.35 %
19
4.85 %
15
3.83 %
2
0.51 %
PAR-RAPID
3
10
0.03 %
3
30.00 %
7
70.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
5
309
0.99 %
8
2.59 %
295
95.47 %
11
3.56 %
6
1.94 %
0
0.00 %
PAR-RAPID
3
7
0.02 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
87
0.28 %
5
5.75 %
79
90.80 %
3
3.45 %
3
3.45 %
0
0.00 %
Pembs
PAR-HERD-S
2
5
0.02 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-OTHER
8
540
1.73 %
42
7.78 %
480
88.89 %
26
4.81 %
18
3.33 %
0
0.00 %
PAR-PERSIS
11
946
3.02 %
62
6.55 %
802
84.78 %
88
9.30 %
79
8.35 %
3
0.32 %
PAR-RAPID
7
27
0.09 %
9
33.33 %
17
62.96 %
1
3.70 %
1
3.70 %
0
0.00 %
SER-FLEXI
2
35
0.11 %
0
0.00 %
33
94.29 %
4
11.43 %
2
5.71 %
0
0.00 %
Powys
PAR-HERD-S
3
10
0.03 %
1
10.00 %
9
90.00 %
2
20.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
7
873
2.79 %
30
3.44 %
735
84.19 %
113
12.94 %
103
11.80 %
5
0.57 %
PAR-PERSIS
8
114
0.36 %
15
13.16 %
54
47.37 %
48
42.11 %
45
39.47 %
0
0.00 %
SER-FLEXI
4
84
0.27 %
2
2.38 %
78
92.86 %
5
5.95 %
4
4.76 %
0
0.00 %
S Wales
PAR-PERSIS
1
69
0.22 %
3
4.35 %
64
92.75 %
6
8.70 %
2
2.90 %
0
0.00 %
SER-FLEXI
1
36
0.12 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.01 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
48
0.15 %
1
2.08 %
47
97.92 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
6
0.02 %
3
50.00 %
2
33.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
9
894
2.86 %
20
2.24 %
820
91.72 %
61
6.82 %
54
6.04 %
0
0.00 %
PAR-PERSIS
3
135
0.43 %
27
20.00 %
103
76.30 %
5
3.70 %
5
3.70 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
2
92
0.29 %
1
1.09 %
89
96.74 %
5
5.43 %
2
2.17 %
0
0.00 %
S Glamorgan
SER-FLEXI
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
1
35
0.11 %
2
5.71 %
29
82.86 %
4
11.43 %
4
11.43 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
1
336
1.07 %
1
0.30 %
327
97.32 %
16
4.76 %
8
2.38 %
0
0.00 %
Wales
117
5757
18.41 %
335
5.82 %
5037
87.49 %
448
7.78 %
375
6.51 %
10
0.17 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
383
1.22 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
3
569
1.82 %
8
1.41 %
538
94.55 %
26
4.57 %
23
4.04 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
1
21
0.07 %
0
0.00 %
21
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
297
0.95 %
14
4.71 %
267
89.90 %
18
6.06 %
16
5.39 %
0
0.00 %
Scotland
10
1270
4.06 %
23
1.81 %
1185
93.31 %
74
5.83 %
62
4.88 %
0
0.00 %
Grand Total
Sum:
343
31274
1367
27799
2068
1611
497
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol January 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
208
23393
96.5%
959
4.1%
20807
88.9%
1500
6.4%
1140
4.9%
487
2.1%
Serial/Extended
8
854
3.5%
50
5.9%
770
90.2%
46
5.4%
34
4.0%
0
0.0 %
Total
216
24247
100.0%
1009
4.2%
21577
89.0%
1546
6.4%
1174
4.8%
487
2.0%
Scotland
Parallel
10
1270
100.0%
23
1.8%
1185
93.3%
74
5.8%
62
4.9%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
10
1270
100.0%
23
1.8%
1185
93.3%
74
5.8%
62
4.9%
0
0.0 %
Wales
Parallel
107
5511
95.7%
327
5.9%
4808
87.2%
436
7.9%
366
6.6%
10
0.2%
Serial/Extended
10
246
4.3%
8
3.3%
229
93.1%
12
4.9%
9
3.7%
0
0.0 %
Total
117
5757
100.0%
335
5.8%
5037
87.5%
448
7.8%
375
6.5%
10
0.2%
GB
Parallel
325
30174
96.5%
1309
4.3%
26800
88.8%
2010
6.7%
1568
5.2%
497
1.6%
Serial/Extended
18
1100
3.5%
58
5.3%
999
90.8%
58
5.3%
43
3.9%
0
0.0 %
Total
343
31274
100.0%
1367
4.4%
27799
88.9%
2068
6.6%
1611
5.2%
497
1.6%
216.00
117.00
10.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
208
23393
96.5%
959
4.1%
20807
88.9%
1500
6.4%
1140
4.9%
487
2.1%
Serial/Extended
8
854
3.5%
50
5.9%
770
90.2%
46
5.4%
34
4.0%
0
0.0 %
Total
216
24247
100.0%
1009
4.2%
21577
89.0%
1546
6.4%
1174
4.8%
487
2.0%
Scotland
Parallel
10
1270
100.0%
23
1.8%
1185
93.3%
74
5.8%
62
4.9%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
10
1270
100.0%
23
1.8%
1185
93.3%
74
5.8%
62
4.9%
0
0.0 %
Wales
Parallel
107
5511
95.7%
327
5.9%
4808
87.2%
436
7.9%
366
6.6%
10
0.2%
Serial/Extended
10
246
4.3%
8
3.3%
229
93.1%
12
4.9%
9
3.7%
0
0.0 %
Total
117
5757
100.0%
335
5.8%
5037
87.5%
448
7.8%
375
6.5%
10
0.2%
GB
Parallel
325
30174
96.5%
1309
4.3%
26800
88.8%
2010
6.7%
1568
5.2%
497
1.6%
Serial/Extended
18
1100
3.5%
58
5.3%
999
90.8%
58
5.3%
43
3.9%
0
0.0 %
Total
343
31274
100.0%
1367
4.4%
27799
88.9%
2068
6.6%
1611
5.2%
497
1.6%
216.00
117.00
10.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
18
1100
3.5%
58
5.3%
999
90.8%
58
5.3 %
43
3.9 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
126
13601
43.5%
400
2.9%
12157
89.4%
893
6.6 %
689
5.1 %
355
2.6 %
Parallel - new infection cull area
85
8782
28.1%
404
4.6%
7762
88.4%
603
6.9 %
481
5.5 %
135
1.5 %
Parallel - Other
13
596
1.9%
49
8.2%
524
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
58
5068
16.2%
325
6.4%
4469
88.2%
339
6.7 %
267
5.3 %
7
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
Parallel - Rapid Testing Two-times IRs
24
61
0.2%
19
31.1%
40
65.6%
2
3.3 %
2
3.3 %
0
Total
343
31274
100.0%
1367
4.4%
27799
88.9%
2068
6.6 %
1611
5.2 %
497
1.6 %
Table 4a. Summaryof IFN Gamma results by submission reason (January 2020)
England - HRA
Extended Flexible IFN-GAMMA test
8
854
2.7%
50
5.9%
770
90.2%
46
5.4 %
34
4.0 %
0
Parallel New Infection
2
244
0.8%
10
4.1%
204
83.6%
37
15.2 %
29
11.9 %
1
0.4 %
Parallel - new infection cull area
83
8400
26.9%
386
4.6%
7421
88.3%
560
6.7 %
458
5.5 %
135
1.6 %
Parallel Persistent Infection
14
2466
7.9%
122
4.9%
2244
91.0%
138
5.6 %
98
4.0 %
2
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
England - HRA
120
14008
44.8%
678
4.8%
12468
89.0%
920
6.6 %
724
5.2 %
138
1.0 %
England - Edge
Parallel New Infection
90
9650
30.9%
310
3.2%
8675
89.9%
575
6.0 %
420
4.4 %
245
2.5 %
Parallel - new infection cull area
2
382
1.2%
18
4.7%
341
89.3%
43
11.3 %
23
6.0 %
0
England - Edge
92
10032
32.1%
328
3.3%
9016
89.9%
618
6.2 %
443
4.4 %
245
2.4 %
England - LRA
Parallel New Infection
4
207
0.7%
3
1.4%
93
44.9%
8
3.9 %
7
3.4 %
104
50.2 %
England - LRA
4
207
0.7%
3
1.4%
93
44.9%
8
3.9 %
7
3.4 %
104
50.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
8
242
0.8%
7
2.9%
226
93.4%
12
5.0 %
9
3.7 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
7
873
2.8%
30
3.4%
735
84.2%
113
12.9 %
103
11.8 %
5
0.6 %
Parallel - Other
12
595
1.9%
49
8.2%
523
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
38
2413
7.7%
172
7.1%
2073
85.9%
195
8.1 %
163
6.8 %
5
0.2 %
Parallel - Rapid Testing Two-times IRs
20
57
0.2%
18
31.6%
37
64.9%
2
3.5 %
2
3.5 %
0
Wales - High TB
91
4202
13.4%
278
6.6%
3613
86.0%
356
8.5 %
301
7.2 %
10
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
11
986
3.2%
21
2.1%
909
92.2%
66
6.7 %
56
5.7 %
0
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
6
189
0.6%
31
16.4%
152
80.4%
6
3.2 %
6
3.2 %
0
Parallel - Rapid Testing Two-times IRs
3
3
0.0%
1
33.3%
2
66.7%
0
0
0
Wales - Intermediate TB
23
1183
3.8%
54
4.6%
1067
90.2%
72
6.1 %
62
5.2 %
0
Wales - Low TB
Parallel New Infection
2
371
1.2%
3
0.8%
356
96.0%
20
5.4 %
12
3.2 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
3
372
1.2%
3
0.8%
357
96.0%
20
5.4 %
12
3.2 %
0
Scotland - Scotland
Parallel New Infection
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0
Scotland - Scotland
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
18
1100
3.5%
58
5.3%
999
90.8%
58
5.3 %
43
3.9 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
126
13601
43.5%
400
2.9%
12157
89.4%
893
6.6 %
689
5.1 %
355
2.6 %
Parallel - new infection cull area
85
8782
28.1%
404
4.6%
7762
88.4%
603
6.9 %
481
5.5 %
135
1.5 %
Parallel - Other
13
596
1.9%
49
8.2%
524
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
58
5068
16.2%
325
6.4%
4469
88.2%
339
6.7 %
267
5.3 %
7
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
Parallel - Rapid Testing Two-times IRs
24
61
0.2%
19
31.1%
40
65.6%
2
3.3 %
2
3.3 %
0
Total
343
31274
100.0%
1367
4.4%
27799
88.9%
2068
6.6 %
1611
5.2 %
497
1.6 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
8
854
2.7%
50
5.9%
770
90.2%
46
5.4 %
34
4.0 %
0
Parallel New Infection
2
244
0.8%
10
4.1%
204
83.6%
37
15.2 %
29
11.9 %
1
0.4 %
Parallel - new infection cull area
83
8400
26.9%
386
4.6%
7421
88.3%
560
6.7 %
458
5.5 %
135
1.6 %
Parallel Persistent Infection
14
2466
7.9%
122
4.9%
2244
91.0%
138
5.6 %
98
4.0 %
2
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
England - Edge
Parallel New Infection
90
9650
30.9%
310
3.2%
8675
89.9%
575
6.0 %
420
4.4 %
245
2.5 %
Parallel - new infection cull area
2
382
1.2%
18
4.7%
341
89.3%
43
11.3 %
23
6.0 %
0
England - LRA
Parallel New Infection
4
207
0.7%
3
1.4%
93
44.9%
8
3.9 %
7
3.4 %
104
50.2 %
England
216
24247
77.5%
1009
4.2%
21577
89.0%
1546
6.4 %
1174
4.8 %
487
2.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
8
242
0.8%
7
2.9%
226
93.4%
12
5.0 %
9
3.7 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
7
873
2.8%
30
3.4%
735
84.2%
113
12.9 %
103
11.8 %
5
0.6 %
Parallel - Other
12
595
1.9%
49
8.2%
523
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
38
2413
7.7%
172
7.1%
2073
85.9%
195
8.1 %
163
6.8 %
5
0.2 %
Parallel - Rapid Testing Two-times IRs
20
57
0.2%
18
31.6%
37
64.9%
2
3.5 %
2
3.5 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
11
986
3.2%
21
2.1%
909
92.2%
66
6.7 %
56
5.7 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2020) Cont.
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
6
189
0.6%
31
16.4%
152
80.4%
6
3.2 %
6
3.2 %
0
Parallel - Rapid Testing Two-times IRs
3
3
0.0%
1
33.3%
2
66.7%
0
0
0
Wales - Low TB
Parallel New Infection
2
371
1.2%
3
0.8%
356
96.0%
20
5.4 %
12
3.2 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
117
5757
18.4%
335
5.8%
5037
87.5%
448
7.8 %
375
6.5 %
10
0.2 %
Scotland - Scotland
Parallel New Infection
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0
Scotland
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
1
0
4
0
773
0
73
0
0
July
316
32549
201
1219
1
13
0
0
0
0
108
3
0
12
0
1020
0
58
0
2
August
262
21887
126
724
1
8
0
0
0
0
84
1
0
6
0
570
0
52
0
0
September
245
25317
134
693
1
7
0
0
0
0
94
1
0
3
0
542
0
45
0
0
October
298
26690
154
743
0
0
0
0
0
58
1
0
5
0
604
0
71
0
3
November
272
30186
169
1080
14
0
0
0
0
72
0
1
15
4
910
4
58
0
0
December
234
25192
138
904
2
1
0
0
0
0
87
0
2
3
1
758
40
2
0
8
2020 January
343
31274
227
1367
43
571
0
0
0
0
60
0
8
0
0
637
27
0
0
0
Totals (last
12 months)
3525
335727
2061
13398
56
646
0
0
0
0
1144
15
11
71
5
10715
77
602
2
20
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2108
267436
1340
10146
506
0
0
0
0
1016
9
8
50
1
8438
7
64
0
1
Scotland
32
3963
16
113
1
0
0
0
0
13
0
1
2
0
90
0
5
0
0
Wales
1385
64328
705
3139
139
0
0
0
0
115
6
2
19
4
2187
70
533
2
19
Totals
3525
335727
2061
13398
646
0
0
0
0
1144
15
11
71
5
10715
77
602
2
20
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
50
100
150
200
250
Wiltshire
W Glamorgan
S Wales
Shropshire
Powys
Oxon
Notts
Norfolk
Leics & Rut
Heref
Gwynedd
Gr Manchester
E Sussex
Dorset
Derbyshire
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during January 2020 (% submission per
County)
County
%Resampled %Retested
Ayrshire
6.01 %
7.83 %
Berks
6.19 %
14.43 %
Bucks
17.91 %
18.58 %
Carms
2.57 %
3.57 %
Ceredigion
4.11 %
5.07 %
Cheshire
3.16 %
4.56 %
Corn & Scilly
3.47 %
4.91 %
Derbyshire
4.79 %
6.46 %
Devon
5.81 %
6.98 %
Dorset
3.62 %
5.34 %
Dumfries & G
4.04 %
4.57 %
E Sussex
2.43 %
3.32 %
Gloucs
7.62 %
9.70 %
Gr Manchester
3.85 %
3.85 %
Gwent
2.23 %
3.47 %
Gwynedd
11.11 %
11.11 %
Hampshire
6.28 %
10.14 %
Heref
7.43 %
8.72 %
Kirkcudbright
Leics & Rut
4.56 %
5.74 %
NE Wales
4.90 %
6.00 %
Norfolk
15.00 %
15.00 %
Northants
6.04 %
7.38 %
Notts
1.33 %
2.67 %
N Yorks
0.71 %
Oxon
4.84 %
6.98 %
Pembs
6.50 %
7.73 %
Powys
13.13 %
14.75 %
S Glamorgan
Shropshire
5.30 %
6.62 %
Somerset excl
4.51 %
6.17 %
S Wales
1.90 %
5.71 %
Warks
2.13 %
3.96 %
W Glamorgan
100.00 %
100.00 %
Wigtown
5.39 %
6.06 %

Wiltshire
5.51 %
7.38 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
23736
1009
4.3%
24247
1009
4.2%
Wales
5668
335
5.9%
5757
335
5.8%
Scotland
1225
23
1.9%
1270
23
1.8%
GB
30629
1367
4.5%
31274
1367
4.4%
Table 6a (i): Number of animals and submissions tested in January 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
23736
1009
4.3%
24247
1009
4.2%
Wales
5668
335
5.9%
5757
335
5.8%
Scotland
1225
23
1.9%
1270
23
1.8%
GB
30629
1367
4.5%
31274
1367
4.4%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
Table 6a (ii): Number of animals and submissions tested in January 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
4
32
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples January 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
4
32
0
Isle of Man
SER-IOM
6
14
0
0
Isle of Man
SER-IOM
6
14
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
17
2
11.8%
Devon
1
630
42
6.7%
North East Wales
3
43
6
14.0%
Pembrokeshire
3
68
9
13.2%
South Wales
1
35
3
8.6%
TC0077
9
793
62
7.8 %
Table 8a Number of Cattle Antibody Tests January 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
17
2
11.8%
Devon
1
630
42
6.7%
North East Wales
3
43
6
14.0%
Pembrokeshire
3
68
9
13.2%
South Wales
1
35
3
8.6%
TC0077
9
793
62
7.8 %
Table 8b Number of Cattle Antibody Tests 2020