Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - June 2023
Source: environment.data.gov.uk (2024-07-03)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2023. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period 1st - 30th June 2023
Issued 21 July 2023
202

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
June 2023
%
Total 2023
%
Submissions
189
1412
Samples
Total (%)
xx-xxxx-xxxx
100.00 %
107637
100.00 %
England
9076
55.07 %
67807
63.00 %
Wales
6123
37.15 %
32791
30.46 %
Scotland
1282
7.78 %
7039
6.54 %
Parallel Tests
PAR-CHR
549
3.33 %
2241
2.08 %
PAR-CUL-N
1292
7.84 %
3864
3.59 %
PAR-CUL-P
0
0.00 %
539
0.50 %
PAR-HERD-S
0
0.00 %
457
0.42 %
PAR-IR-EX
31
0.19 %
268
0.25 %
PAR-IR-SEV
170
1.03 %
1261
1.17 %
PAR-NEW-IN
3414
20.71 %
36238
33.67 %
PAR-OTHER
257
1.56 %
1780
1.65 %
PAR-PERSIS
1461
8.86 %
8821
8.20 %
PAR-POSTM
0
0.00 %
94
0.09 %
PAR-RAPID
62
0.38 %
412
0.38 %
PAR-RECUR
5590
33.92 %
44478
41.32 %
Total(% of all samples)
12826
77.82 %
100453
93.33 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
2
0.00 %
SER-FLEXI
3653
22.16 %
7166
6.66 %
SER-FRAUD
0
0.00 %
14
0.01 %
SER-NSR
0
0.00 %
SER-OTHER
2
0.01 %
2
0.00 %
Total(% of all samples)
3655
22.18 %
7184
6.67 %
Total
xx-xxxx-xxxx
100.00 %
107637
100.00 %
Retests
Total (% of all samples)
1120
6.80 %
7944
7.38 %
England
726
4.41 %
4882
4.54 %
Wales
322
1.95 %
2432
2.26 %
Scotland
72
0.44 %
630
0.59 %
Resamples
Total (% of all samples)
839
5.09 %
6470
6.01 %
England
540
3.28 %
4081
3.79 %
Wales
247
1.50 %
1822
1.69 %
Scotland
52
0.32 %
567
0.53 %
Rejects
Total (% of all samples)
105
0.64 %
xx-xxxx-xxxx
1.57 %
England
66
0.40 %
1167
1.08 %
Wales
39
0.24 %
449
0.42 %
Scotland
0
0.00 %
74
0.07 %
Table 1. Monthly Totals for June 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-OTHER
Breakdown of
Reason for
submission
PAR-CHR
549
3.3 %
PAR-CUL-N
1292
7.8 %
PAR-IR-EX
31
0.2 %
PAR-IR-SEV
170
1.0 %
PAR-NEW-IN
3414
20.7 %
PAR-OTHER
257
1.6 %
PAR-PERSIS
1461
8.9 %
PAR-RAPID
62
0.4 %
PAR-RECUR
5590
33.9 %
SER-FLEXI
3653
22.2 %
SER-OTHER
2
0.0 %
Total
xx-xxxx-xxxx
100.00 %
Figure 1. Number of samples submitted in each category during June 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
Wigtown
Renfrew
Perthshire
Kirkcudbright
Powys
Pembs
NE Wales
Gwynedd
Gwent
Conwy
Ceredigion
Carms
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
Notts
Lincs
Leics & Rut
Heref
Essex
Dorset
Devon
Cumbria
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during June 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
Wigtown
West lothian
Renfrew
Perthshire
Kirkcudbright
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NW Wales
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Conwy
Cheshire
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-FRAUD
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
PAR-RECUR
1
51
0.31 %
1
1.96 %
49
96.08 %
2
3.92 %
1
1.96 %
0
0.00 %
Avon
PAR-RECUR
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-PERSIS
2
13
0.08 %
0
0.00 %
12
92.31 %
2
15.38 %
1
7.69 %
0
0.00 %
PAR-RECUR
6
455
2.76 %
44
9.67 %
369
81.10 %
52
11.43 %
42
9.23 %
0
0.00 %
Devon
PAR-CHR
2
408
2.48 %
11
2.70 %
373
91.42 %
39
9.56 %
24
5.88 %
0
0.00 %
PAR-CUL-N
1
305
1.85 %
7
2.30 %
286
93.77 %
13
4.26 %
11
3.61 %
1
0.33 %
PAR-PERSIS
1
19
0.12 %
0
0.00 %
13
68.42 %
6
31.58 %
6
31.58 %
0
0.00 %
PAR-RECUR
8
1,648
10.00 %
33
2.00 %
1459
88.53 %
185
11.23 %
156
9.47 %
0
0.00 %
Dorset
PAR-OTHER
1
254
1.54 %
21
8.27 %
224
88.19 %
6
2.36 %
3
1.18 %
6
2.36 %
Heref
PAR-RECUR
10
1,470
8.92 %
59
4.01 %
1322
89.93 %
125
8.50 %
89
6.05 %
0
0.00 %
Shropshire
PAR-RECUR
5
635
3.85 %
27
4.25 %
593
93.39 %
16
2.52 %
14
2.20 %
1
0.16 %
Somerset excl N
PAR-CHR
2
129
0.78 %
13
10.08 %
105
81.40 %
17
13.18 %
11
8.53 %
0
0.00 %
PAR-RECUR
6
413
2.51 %
38
9.20 %
359
86.92 %
20
4.84 %
15
3.63 %
1
0.24 %
Staffs
PAR-CHR
1
12
0.07 %
0
0.00 %
8
66.67 %
4
33.33 %
4
33.33 %
0
0.00 %
PAR-RECUR
2
516
3.13 %
2
0.39 %
447
86.63 %
102
19.77 %
63
12.21 %
4
0.78 %
Wiltshire
PAR-CUL-N
2
987
5.99 %
32
3.24 %
921
93.31 %
42
4.26 %
31
3.14 %
3
0.30 %
PAR-RECUR
1
69
0.42 %
2
2.90 %
57
82.61 %
10
14.49 %
10
14.49 %
0
0.00 %
England - Edge
Bucks
PAR-NEW-IN
2
143
0.87 %
3
2.10 %
87
60.84 %
4
2.80 %
3
2.10 %
50
34.97 %
Cheshire
PAR-RECUR
1
202
1.23 %
2
0.99 %
196
97.03 %
6
2.97 %
4
1.98 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
207
1.26 %
2
0.97 %
203
98.07 %
5
2.42 %
2
0.97 %
0
0.00 %
Notts
PAR-NEW-IN
2
252
1.53 %
3
1.19 %
231
91.67 %
23
9.13 %
18
7.14 %
0
0.00 %
Oxon
PAR-PERSIS
2
741
4.50 %
25
3.37 %
690
93.12 %
41
5.53 %
26
3.51 %
0
0.00 %
PAR-RECUR
2
75
0.46 %
0
0.00 %
73
97.33 %
2
2.67 %
2
2.67 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
7
0.04 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
Essex
PAR-NEW-IN
2
52
0.32 %
6
11.54 %
43
82.69 %
3
5.77 %
3
5.77 %
0
0.00 %
Lincs
PAR-NEW-IN
1
11
0.07 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2a. Summary by County for June 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for June 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England
67
9076
55.07 %
331
3.65 %
8139
89.68 %
726
8.00 %
540
5.95 %
66
0.73 %
Wales - High TB
Carms
PAR-IR-EX
2
6
0.04 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-IR-SEV
2
12
0.07 %
6
50.00 %
6
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
5
331
2.01 %
12
3.63 %
310
93.66 %
12
3.63 %
9
2.72 %
0
0.00 %
PAR-RAPID
5
15
0.09 %
8
53.33 %
7
46.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
44
0.27 %
3
6.82 %
31
70.45 %
10
22.73 %
8
18.18 %
2
4.55 %
Ceredigion
PAR-IR-EX
2
5
0.03 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-IR-SEV
6
46
0.28 %
5
10.87 %
40
86.96 %
2
4.35 %
1
2.17 %
0
0.00 %
PAR-RAPID
4
8
0.05 %
2
25.00 %
5
62.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Gwent
PAR-IR-SEV
2
3
0.02 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-RAPID
2
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
748
4.54 %
14
1.87 %
710
94.92 %
24
3.21 %
19
2.54 %
5
0.67 %
Pembs
PAR-IR-SEV
9
86
0.52 %
12
13.95 %
71
82.56 %
4
4.65 %
3
3.49 %
0
0.00 %
PAR-PERSIS
2
220
1.33 %
6
2.73 %
208
94.55 %
6
2.73 %
5
2.27 %
1
0.45 %
PAR-RAPID
11
28
0.17 %
1
3.57 %
27
96.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
6
0.04 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
SER-FLEXI
5
561
3.40 %
12
2.14 %
535
95.37 %
19
3.39 %
14
2.50 %
0
0.00 %
Powys
PAR-IR-EX
1
8
0.05 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
7
0.04 %
0
0.00 %
7
100.00 %
2
28.57 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
137
0.83 %
2
1.46 %
126
91.97 %
11
8.03 %
9
6.57 %
0
0.00 %
PAR-RAPID
3
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
2,344
14.22 %
52
2.22 %
2186
93.26 %
93
3.97 %
78
3.33 %
28
1.19 %
Wales - Intermediate TB
NE Wales
PAR-IR-EX
2
7
0.04 %
0
0.00 %
5
71.43 %
3
42.86 %
2
28.57 %
0
0.00 %
PAR-IR-SEV
2
10
0.06 %
3
30.00 %
7
70.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
5
559
3.39 %
8
1.43 %
512
91.59 %
43
7.69 %
38
6.80 %
1
0.18 %
PAR-RAPID
2
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for June 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-IR-SEV
1
6
0.04 %
1
16.67 %
4
66.67 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-NEW-IN
2
13
0.08 %
0
0.00 %
10
76.92 %
4
30.77 %
3
23.08 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
2
8
0.05 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Gwynedd
PAR-NEW-IN
11
393
2.38 %
8
2.04 %
349
88.80 %
55
13.99 %
36
9.16 %
0
0.00 %
NE Wales
PAR-IR-EX
1
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
9
491
2.98 %
3
0.61 %
471
95.93 %
27
5.50 %
15
3.05 %
2
0.41 %
Wales
115
6123
37.15 %
163
2.66 %
5674
92.67 %
322
5.26 %
247
4.03 %
39
0.64 %
Scotland - Scotland
Kirkcudbright
PAR-NEW-IN
3
1,116
6.77 %
30
2.69 %
1059
94.89 %
40
3.58 %
27
2.42 %
0
0.00 %
Perthshire
PAR-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Renfrew
SER-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
Wigtown
PAR-NEW-IN
2
162
0.98 %
0
0.00 %
137
84.57 %
31
19.14 %
25
15.43 %
0
0.00 %
Scotland
7
1282
7.78 %
30
2.34 %
1200
93.60 %
72
5.62 %
52
4.06 %
0
0.00 %
Grand Total
Sum:
189
xx-xxxx-xxxx
524
15013
1120
839
105
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
PAR-RECUR
1
51
0.05 %
1
1.96 %
49
96.08 %
2
3.92 %
1
1.96 %
0
0.00 %
Avon
PAR-RECUR
13
993
0.92 %
33
3.32 %
913
91.94 %
51
5.14 %
45
4.53 %
2
0.20 %
Corn & Scilly
PAR-CUL-N
3
926
0.86 %
60
6.48 %
804
86.83 %
80
8.64 %
62
6.70 %
0
0.00 %
PAR-CUL-P
1
539
0.50 %
31
5.75 %
481
89.24 %
32
5.94 %
26
4.82 %
1
0.19 %
PAR-HERD-S
1
61
0.06 %
1
1.64 %
59
96.72 %
4
6.56 %
1
1.64 %
0
0.00 %
PAR-OTHER
3
324
0.30 %
24
7.41 %
271
83.64 %
33
10.19 %
29
8.95 %
0
0.00 %
PAR-PERSIS
6
554
0.51 %
42
7.58 %
473
85.38 %
45
8.12 %
39
7.04 %
0
0.00 %
PAR-RECUR
44
8,821
8.20 %
363
4.12 %
7844
88.92 %
703
7.97 %
611
6.93 %
3
0.03 %
Devon
PAR-CHR
2
408
0.38 %
11
2.70 %
373
91.42 %
39
9.56 %
24
5.88 %
0
0.00 %
PAR-CUL-N
10
1,711
1.59 %
81
4.73 %
1536
89.77 %
105
6.14 %
87
5.08 %
7
0.41 %
PAR-HERD-S
2
396
0.37 %
35
8.84 %
326
82.32 %
48
12.12 %
35
8.84 %
0
0.00 %
PAR-PERSIS
4
937
0.87 %
38
4.06 %
873
93.17 %
36
3.84 %
26
2.77 %
0
0.00 %
PAR-RECUR
43
7,504
6.97 %
417
5.56 %
6375
84.95 %
551
7.34 %
445
5.93 %
267
3.56 %
Dorset
PAR-OTHER
1
254
0.24 %
21
8.27 %
224
88.19 %
6
2.36 %
3
1.18 %
6
2.36 %
PAR-RECUR
2
717
0.67 %
12
1.67 %
321
44.77 %
22
3.07 %
22
3.07 %
362
50.49 %
Gloucs
PAR-CUL-N
2
35
0.03 %
5
14.29 %
30
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
541
0.50 %
25
4.62 %
479
88.54 %
43
7.95 %
37
6.84 %
0
0.00 %
Heref
PAR-OTHER
2
83
0.08 %
3
3.61 %
74
89.16 %
6
7.23 %
6
7.23 %
0
0.00 %
PAR-RECUR
28
5,230
4.86 %
288
5.51 %
4619
88.32 %
395
7.55 %
323
6.18 %
0
0.00 %
Shropshire
PAR-RECUR
35
3,595
3.34 %
152
4.23 %
3198
88.96 %
295
8.21 %
242
6.73 %
3
0.08 %
Somerset excl N
PAR-CHR
4
271
0.25 %
35
12.92 %
217
80.07 %
29
10.70 %
19
7.01 %
0
0.00 %
PAR-OTHER
2
12
0.01 %
2
16.67 %
9
75.00 %
2
16.67 %
1
8.33 %
0
0.00 %
PAR-PERSIS
2
296
0.27 %
47
15.88 %
226
76.35 %
25
8.45 %
23
7.77 %
0
0.00 %
PAR-RECUR
24
2,973
2.76 %
193
6.49 %
2678
90.08 %
120
4.04 %
98
3.30 %
4
0.13 %
Staffs
PAR-CHR
7
1,562
1.45 %
57
3.65 %
1370
87.71 %
149
9.54 %
134
8.58 %
1
0.06 %
PAR-OTHER
1
38
0.04 %
1
2.63 %
37
97.37 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2b. Summary by County for 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
27
5,603
5.21 %
114
2.03 %
5044
90.02 %
499
8.91 %
425
7.59 %
20
0.36 %
Wiltshire
PAR-CUL-N
3
992
0.92 %
34
3.43 %
924
93.15 %
42
4.23 %
31
3.13 %
3
0.30 %
PAR-RECUR
11
1,448
1.35 %
56
3.87 %
1327
91.64 %
81
5.59 %
61
4.21 %
4
0.28 %
England - Edge
Berks
PAR-NEW-IN
4
784
0.73 %
10
1.28 %
726
92.60 %
50
6.38 %
48
6.12 %
0
0.00 %
PAR-RECUR
1
132
0.12 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-CUL-N
1
191
0.18 %
3
1.57 %
182
95.29 %
6
3.14 %
6
3.14 %
0
0.00 %
PAR-NEW-IN
18
1,791
1.66 %
47
2.62 %
1623
90.62 %
77
4.30 %
70
3.91 %
51
2.85 %
PAR-RECUR
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Cheshire
PAR-OTHER
3
516
0.48 %
16
3.10 %
489
94.77 %
11
2.13 %
11
2.13 %
0
0.00 %
PAR-PERSIS
8
1,187
1.10 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
PAR-RECUR
3
417
0.39 %
4
0.96 %
397
95.20 %
21
5.04 %
16
3.84 %
0
0.00 %
Derbyshire
PAR-NEW-IN
10
684
0.64 %
38
5.56 %
603
88.16 %
53
7.75 %
43
6.29 %
0
0.00 %
PAR-RECUR
6
1,306
1.21 %
36
2.76 %
1185
90.74 %
93
7.12 %
85
6.51 %
0
0.00 %
E Sussex
PAR-NEW-IN
13
1,074
1.00 %
21
1.96 %
986
91.81 %
82
7.64 %
67
6.24 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
799
0.74 %
32
4.01 %
724
90.61 %
52
6.51 %
41
5.13 %
2
0.25 %
Leics & Rut
PAR-NEW-IN
17
2,119
1.97 %
58
2.74 %
1975
93.20 %
105
4.96 %
86
4.06 %
0
0.00 %
PAR-RECUR
1
135
0.13 %
11
8.15 %
120
88.89 %
7
5.19 %
4
2.96 %
0
0.00 %
Northants
PAR-NEW-IN
11
1,176
1.09 %
25
2.13 %
951
80.87 %
95
8.08 %
81
6.89 %
119
10.12 %
PAR-RECUR
2
408
0.38 %
6
1.47 %
374
91.67 %
32
7.84 %
28
6.86 %
0
0.00 %
Notts
PAR-NEW-IN
9
553
0.51 %
13
2.35 %
501
90.60 %
60
10.85 %
37
6.69 %
2
0.36 %
Oxon
PAR-NEW-IN
3
250
0.23 %
4
1.60 %
228
91.20 %
23
9.20 %
18
7.20 %
0
0.00 %
PAR-PERSIS
3
976
0.91 %
39
4.00 %
895
91.70 %
57
5.84 %
42
4.30 %
0
0.00 %
PAR-RECUR
11
804
0.75 %
46
5.72 %
712
88.56 %
55
6.84 %
46
5.72 %
0
0.00 %
SER-FLEXI
2
55
0.05 %
3
5.45 %
48
87.27 %
4
7.27 %
4
7.27 %
0
0.00 %
Warks
PAR-NEW-IN
7
683
0.63 %
22
3.22 %
638
93.41 %
34
4.98 %
23
3.37 %
0
0.00 %
PAR-OTHER
1
19
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
19
100.00 %
PAR-RECUR
10
152
0.14 %
5
3.29 %
116
76.32 %
19
12.50 %
17
11.18 %
14
9.21 %
England - LRA
Cumbria
PAR-NEW-IN
12
2,415
2.24 %
68
2.82 %
2155
89.23 %
215
8.90 %
192
7.95 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Essex
PAR-NEW-IN
6
405
0.38 %
7
1.73 %
216
53.33 %
7
1.73 %
5
1.23 %
177
43.70 %
Gr Manchester
PAR-PERSIS
1
41
0.04 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
Humberside
PAR-NEW-IN
2
68
0.06 %
1
1.47 %
60
88.24 %
10
14.71 %
6
8.82 %
1
1.47 %
Lancashire
PAR-NEW-IN
1
75
0.07 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Lincs
PAR-NEW-IN
9
961
0.89 %
18
1.87 %
862
89.70 %
85
8.84 %
81
8.43 %
0
0.00 %
N Yorks
PAR-NEW-IN
3
179
0.17 %
2
1.12 %
170
94.97 %
8
4.47 %
7
3.91 %
0
0.00 %
Surrey
PAR-NEW-IN
4
507
0.47 %
22
4.34 %
358
70.61 %
29
5.72 %
29
5.72 %
98
19.33 %
W Yorks
PAR-NEW-IN
1
69
0.06 %
0
0.00 %
65
94.20 %
5
7.25 %
4
5.80 %
0
0.00 %
England
480
67807
63.00 %
2775
4.09 %
59784
88.17 %
4882
7.20 %
4081
6.02 %
1167
1.72 %
Wales - High TB
Carms
PAR-IR-EX
8
42
0.04 %
10
23.81 %
31
73.81 %
1
2.38 %
1
2.38 %
0
0.00 %
PAR-IR-SEV
22
145
0.13 %
62
42.76 %
76
52.41 %
7
4.83 %
7
4.83 %
0
0.00 %
PAR-OTHER
3
82
0.08 %
1
1.22 %
75
91.46 %
7
8.54 %
6
7.32 %
0
0.00 %
PAR-PERSIS
12
751
0.70 %
32
4.26 %
684
91.08 %
39
5.19 %
27
3.60 %
8
1.07 %
PAR-RAPID
38
98
0.09 %
32
32.65 %
61
62.24 %
5
5.10 %
4
4.08 %
1
1.02 %
PAR-RECUR
8
491
0.46 %
40
8.15 %
426
86.76 %
26
5.30 %
22
4.48 %
3
0.61 %
SER-ANOM
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
11
695
0.65 %
50
7.19 %
610
87.77 %
41
5.90 %
32
4.60 %
3
0.43 %
SER-FRAUD
1
14
0.01 %
0
0.00 %
13
92.86 %
1
7.14 %
1
7.14 %
0
0.00 %
Ceredigion
PAR-IR-EX
8
33
0.03 %
5
15.15 %
24
72.73 %
4
12.12 %
4
12.12 %
0
0.00 %
PAR-IR-SEV
16
98
0.09 %
15
15.31 %
78
79.59 %
6
6.12 %
5
5.10 %
0
0.00 %
PAR-PERSIS
5
630
0.59 %
29
4.60 %
585
92.86 %
20
3.17 %
14
2.22 %
2
0.32 %
PAR-RAPID
12
24
0.02 %
6
25.00 %
16
66.67 %
2
8.33 %
2
8.33 %
0
0.00 %
PAR-RECUR
3
147
0.14 %
6
4.08 %
137
93.20 %
3
2.04 %
3
2.04 %
1
0.68 %
SER-FLEXI
9
490
0.46 %
86
17.55 %
380
77.55 %
37
7.55 %
24
4.90 %
0
0.00 %
Gwent
PAR-IR-EX
2
14
0.01 %
9
64.29 %
5
35.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
19
0.02 %
10
52.63 %
7
36.84 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-OTHER
1
6
0.01 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
12
28
0.03 %
5
17.86 %
21
75.00 %
3
10.71 %
2
7.14 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
5
388
0.36 %
22
5.67 %
352
90.72 %
21
5.41 %
13
3.35 %
1
0.26 %
SER-FLEXI
12
1,467
1.36 %
63
4.29 %
1342
91.48 %
69
4.70 %
53
3.61 %
9
0.61 %
Pembs
PAR-IR-EX
27
106
0.10 %
46
43.40 %
56
52.83 %
3
2.83 %
3
2.83 %
1
0.94 %
PAR-IR-SEV
95
672
0.62 %
205
30.51 %
427
63.54 %
47
6.99 %
29
4.32 %
11
1.64 %
PAR-OTHER
2
48
0.04 %
5
10.42 %
42
87.50 %
1
2.08 %
1
2.08 %
0
0.00 %
PAR-PERSIS
23
1,593
1.48 %
119
7.47 %
1358
85.25 %
138
8.66 %
110
6.91 %
6
0.38 %
PAR-RAPID
54
164
0.15 %
35
21.34 %
121
73.78 %
10
6.10 %
7
4.27 %
1
0.61 %
PAR-RECUR
18
1,304
1.21 %
67
5.14 %
1110
85.12 %
107
8.21 %
88
6.75 %
39
2.99 %
SER-FLEXI
23
1,864
1.73 %
85
4.56 %
xx-xxxx-xxxx
91.04 %
125
6.71 %
80
4.29 %
2
0.11 %
Powys
PAR-IR-EX
6
16
0.01 %
1
6.25 %
13
81.25 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-IR-SEV
17
116
0.11 %
22
18.97 %
84
72.41 %
15
12.93 %
9
7.76 %
1
0.86 %
PAR-NEW-IN
2
152
0.14 %
13
8.55 %
131
86.18 %
10
6.58 %
8
5.26 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
8
1,375
1.28 %
31
2.25 %
1281
93.16 %
34
2.47 %
27
1.96 %
36
2.62 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
20
33
0.03 %
4
12.12 %
29
87.88 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
10
349
0.32 %
16
4.58 %
272
77.94 %
63
18.05 %
58
16.62 %
3
0.86 %
SER-FLEXI
5
2,344
2.18 %
52
2.22 %
2186
93.26 %
93
3.97 %
78
3.33 %
28
1.19 %
S Wales
PAR-IR-SEV
2
21
0.02 %
12
57.14 %
9
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
4
121
0.11 %
6
4.96 %
99
81.82 %
19
15.70 %
15
12.40 %
1
0.83 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
253
0.24 %
43
17.00 %
195
77.08 %
15
5.93 %
14
5.53 %
1
0.40 %
PAR-RAPID
4
9
0.01 %
4
44.44 %
5
55.56 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
7
493
0.46 %
23
4.67 %
442
89.66 %
27
5.48 %
23
4.67 %
5
1.01 %
Ceredigion
PAR-IR-EX
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
341
0.32 %
45
13.20 %
271
79.47 %
29
8.50 %
25
7.33 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
12
0.01 %
0
0.00 %
9
75.00 %
5
41.67 %
3
25.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
NE Wales
PAR-IR-EX
17
44
0.04 %
8
18.18 %
31
70.45 %
6
13.64 %
5
11.36 %
0
0.00 %
PAR-IR-SEV
18
169
0.16 %
29
17.16 %
137
81.07 %
3
1.78 %
3
1.78 %
0
0.00 %
PAR-NEW-IN
47
3,682
3.42 %
91
2.47 %
3315
90.03 %
289
7.85 %
255
6.93 %
21
0.57 %
PAR-OTHER
1
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
128
0.12 %
7
5.47 %
106
82.81 %
18
14.06 %
15
11.72 %
0
0.00 %
PAR-POSTM
12
65
0.06 %
6
9.23 %
56
86.15 %
4
6.15 %
3
4.62 %
0
0.00 %
PAR-RAPID
16
32
0.03 %
6
18.75 %
25
78.13 %
1
3.13 %
1
3.13 %
0
0.00 %
PAR-RECUR
1
56
0.05 %
1
1.79 %
50
89.29 %
6
10.71 %
5
8.93 %
0
0.00 %
SER-FLEXI
4
86
0.08 %
6
6.98 %
75
87.21 %
5
5.81 %
5
5.81 %
0
0.00 %
Powys
PAR-IR-EX
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
9
0.01 %
2
22.22 %
6
66.67 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
8
560
0.52 %
17
3.04 %
511
91.25 %
40
7.14 %
31
5.54 %
1
0.18 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
128
0.12 %
0
0.00 %
118
92.19 %
13
10.16 %
10
7.81 %
0
0.00 %
S Glamorgan
PAR-RAPID
3
6
0.01 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-PERSIS
2
5
0.00 %
0
0.00 %
5
100.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
3
217
0.20 %
0
0.00 %
208
95.85 %
13
5.99 %
8
3.69 %
1
0.46 %
Gwynedd
PAR-IR-SEV
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
85
5,913
5.49 %
150
2.54 %
5344
90.38 %
505
8.54 %
395
6.68 %
24
0.41 %
PAR-OTHER
2
11
0.01 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-PERSIS
1
7
0.01 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
5
5
0.00 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
291
0.27 %
10
3.44 %
267
91.75 %
18
6.19 %
13
4.47 %
1
0.34 %
NE Wales
PAR-IR-EX
3
8
0.01 %
2
25.00 %
6
75.00 %
1
12.50 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
2
5
0.00 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
56
4,060
3.77 %
86
2.12 %
3488
85.91 %
446
10.99 %
248
6.11 %
238
5.86 %
PAR-POSTM
9
23
0.02 %
3
13.04 %
20
86.96 %
1
4.35 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
4
165
0.15 %
12
7.27 %
138
83.64 %
17
10.30 %
15
9.09 %
0
0.00 %
NW Wales
PAR-NEW-IN
2
24
0.02 %
0
0.00 %
23
95.83 %
1
4.17 %
1
4.17 %
0
0.00 %
Wales
867
32791
30.46 %
1772
5.40 %
28748
87.67 %
2432
7.42 %
1822
5.56 %
449
1.37 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
14
1,338
1.24 %
86
6.43 %
1104
82.51 %
160
11.96 %
143
10.69 %
5
0.37 %
Kirkcudbright
PAR-CUL-N
1
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
18
3,521
3.27 %
155
4.40 %
3129
88.87 %
268
7.61 %
237
6.73 %
0
0.00 %
Perthshire
PAR-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Renfrew
SER-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
West lothian
PAR-NEW-IN
9
797
0.74 %
31
3.89 %
640
80.30 %
61
7.65 %
58
7.28 %
68
8.53 %
Wigtown
PAR-NEW-IN
21
1,370
1.27 %
110
8.03 %
1131
82.55 %
139
10.15 %
128
9.34 %
1
0.07 %
Scotland
65
7039
6.54 %
382
5.43 %
6016
85.47 %
630
8.95 %
567
8.06 %
74
1.05 %
Grand Total
Sum:
xx-xxxx-xxxx
4929
94548
7944
6470
xx-xxxx-xxxx
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol June 2023
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
67
9076
100.0%
331
3.6%
8139
89.7%
726
8.0%
540
5.9%
66
0.7%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
67
9076
100.0%
331
3.6%
8139
89.7%
726
8.0%
540
5.9%
66
0.7%
Scotland
Parallel
6
1280
99.8%
30
2.3%
1198
93.6%
71
5.5%
52
4.1%
0
0.0 %
Serial/Extended
1
2
0.2%
0
0.0 %
2 100.0%
1
50.0%
0
0.0 %
0
0.0 %
Total
7
1282
100.0%
30
2.3%
1200
93.6%
72
5.6%
52
4.1%
0
0.0 %
Wales
Parallel
102
2470
40.3%
85
3.4%
2243
90.8%
186
7.5%
136
5.5%
6
0.2%
Serial/Extended
13
3653
59.7%
78
2.1%
3431
93.9%
136
3.7%
111
3.0%
33
0.9%
Total
115
6123
100.0%
163
2.7%
5674
92.7%
322
5.3%
247
4.0%
39
0.6%
GB
Parallel
175
12826
77.8%
446
3.5%
11580
90.3%
983
7.7%
728
5.7%
72
0.6%
Serial/Extended
14
3655
22.2%
78
2.1%
3433
93.9%
137
3.7%
111
3.0%
33
0.9%
Total
189
xx-xxxx-xxxx
100.0%
524
3.2%
15013
91.1%
1120
6.8%
839
5.1%
105
0.6%
67.00
115.00
7.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2023)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
478
67752
99.9%
2772
4.1%
59736
88.2%
4878
7.2%
4077
6.0%
1167
1.7%
Serial/Extended
2
55
0.1%
3
5.5%
48
87.3%
4
7.3%
4
7.3%
0
0.0 %
Total
480
67807
100.0%
2775
4.1%
59784
88.2%
4882
7.2%
4081
6.0%
1167
1.7%
Scotland
Parallel
64
7037
100.0%
382
5.4%
6014
85.5%
629
8.9%
567
8.1%
74
1.1%
Serial/Extended
1
2
0.0%
0
0.0 %
2 100.0%
1
50.0%
0
0.0 %
0
0.0 %
Total
65
7039
100.0%
382
5.4%
6016
85.5%
630
9.0%
567
8.1%
74
1.1%
Wales
Parallel
796
25664
78.3%
1418
5.5%
22305
86.9%
2044
8.0%
1534
6.0%
407
1.6%
Serial/Extended
71
7127
21.7%
354
5.0%
6443
90.4%
388
5.4%
288
4.0%
42
0.6%
Total
867
32791
100.0%
1772
5.4%
28748
87.7%
2432
7.4%
1822
5.6%
449
1.4%
GB
Parallel
xx-xxxx-xxxx
93.3%
4572
4.6%
88055
87.7%
7551
7.5%
6178
6.2%
xx-xxxx-xxxx
1.6%
Serial/Extended
74
7184
6.7%
357
5.0%
6493
90.4%
393
5.5%
292
4.1%
42
0.6%
Total
xx-xxxx-xxxx
100.0%
4929
4.6%
94548
87.8%
7944
7.4%
6470
6.0%
xx-xxxx-xxxx
1.6%
480.00
867.00
65.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
3653
22.2%
78
2.1%
3431
93.9%
136
3.7 %
111
3.0 %
33
0.9 %
Parallel – chronic breakdown
5
549
3.3%
24
4.4%
486
88.5%
60
10.9 %
39
7.1 %
0
Parallel - IRs in Exit Strategy for persistent her
8
31
0.2%
2
6.5%
25
80.6%
5
16.1 %
4
12.9 %
0
Parallel New Infection
44
3414
20.7%
63
1.8%
3126
91.6%
237
6.9 %
172
5.0 %
53
1.6 %
Parallel - new infection cull area
3
1292
7.8%
39
3.0%
1207
93.4%
55
4.3 %
42
3.3 %
4
0.3 %
Parallel - Other
3
257
1.6%
21
8.2%
227
88.3%
6
2.3 %
3
1.2 %
6
2.3 %
Parallel Persistent Infection
15
1461
8.9%
45
3.1%
1359
93.0%
78
5.3 %
56
3.8 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
27
62
0.4%
12
19.4%
49
79.0%
1
1.6 %
1
1.6 %
0
Parallel - Recurrent breakdowns
46
5590
33.9%
212
3.8%
4965
88.8%
531
9.5 %
405
7.2 %
8
0.1 %
Parallel - severe IRs - in persistent herds
24
170
1.0%
28
16.5%
136
80.0%
10
5.9 %
6
3.5 %
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Total
189
xx-xxxx-xxxx
100.0%
524
3.2%
15013
91.1%
1120
6.8 %
839
5.1 %
105
0.6 %
Table 4a. Summaryof IFN Gamma results by submission reason (June 2023)
England - HRA
Parallel – chronic breakdown
5
549
3.3%
24
4.4%
486
88.5%
60
10.9 %
39
7.1 %
0
Parallel - new infection cull area
3
1292
7.8%
39
3.0%
1207
93.4%
55
4.3 %
42
3.3 %
4
0.3 %
Parallel - Other
1
254
1.5%
21
8.3%
224
88.2%
6
2.4 %
3
1.2 %
6
2.4 %
Parallel Persistent Infection
3
32
0.2%
0
0.0 %
25
78.1%
8
25.0 %
7
21.9 %
0
Parallel - Recurrent breakdowns
40
5259
31.9%
206
3.9%
4657
88.6%
512
9.7 %
390
7.4 %
6
0.1 %
England - HRA
52
7386
44.8%
290
3.9%
6599
89.3%
641
8.7 %
481
6.5 %
16
0.2 %
England - Edge
Parallel New Infection
6
602
3.7%
8
1.3%
521
86.5%
32
5.3 %
23
3.8 %
50
8.3 %
Parallel Persistent Infection
2
741
4.5%
25
3.4%
690
93.1%
41
5.5 %
26
3.5 %
0
Parallel - Recurrent breakdowns
3
277
1.7%
2
0.7%
269
97.1%
8
2.9 %
6
2.2 %
0
England - Edge
11
1620
9.8%
35
2.2%
1480
91.4%
81
5.0 %
55
3.4 %
50
3.1 %
England - LRA
Parallel New Infection
4
70
0.4%
6
8.6%
60
85.7%
4
5.7 %
4
5.7 %
0
England - LRA
4
70
0.4%
6
8.6%
60
85.7%
4
5.7 %
4
5.7 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
13
3653
22.2%
78
2.1%
3431
93.9%
136
3.7 %
111
3.0 %
33
0.9 %
Parallel - IRs in Exit Strategy for persistent her
5
19
0.1%
2
10.5%
15
78.9%
2
10.5 %
2
10.5 %
0
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
10
688
4.2%
20
2.9%
644
93.6%
29
4.2 %
23
3.3 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
25
59
0.4%
12
20.3%
46
78.0%
1
1.7 %
1
1.7 %
0
Parallel - Recurrent breakdowns
3
54
0.3%
4
7.4%
39
72.2%
11
20.4 %
9
16.7 %
2
3.7 %
Parallel - severe IRs - in persistent herds
21
154
0.9%
24
15.6%
125
81.2%
9
5.8 %
5
3.2 %
0
Wales - High TB
78
4628
28.1%
140
3.0%
4301
92.9%
188
4.1 %
151
3.3 %
36
0.8 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
2
7
0.0%
0
0.0 %
5
71.4%
3
42.9 %
2
28.6 %
0
Parallel New Infection
7
572
3.5%
8
1.4%
522
91.3%
47
8.2 %
41
7.2 %
1
0.2 %
Parallel - Rapid Testing Two-times IRs
2
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Parallel - severe IRs - in persistent herds
3
16
0.1%
4
25.0%
11
68.8%
1
6.3 %
1
6.3 %
0
Wales - Intermediate TB
14
598
3.6%
12
2.0%
541
90.5%
51
8.5 %
44
7.4 %
1
0.2 %
Wales - Low TB
Parallel - IRs in Exit Strategy for persistent her
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Parallel New Infection
22
892
5.4%
11
1.2%
827
92.7%
83
9.3 %
52
5.8 %
2
0.2 %
Wales - Low TB
23
897
5.4%
11
1.2%
832
92.8%
83
9.3 %
52
5.8 %
2
0.2 %
Scotland - Scotland
Parallel New Infection
5
1278
7.8%
30
2.3%
1196
93.6%
71
5.6 %
52
4.1 %
0
Parallel - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Scotland - Scotland
7
1282
7.8%
30
2.3%
1200
93.6%
72
5.6 %
52
4.1 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
70
7166
6.7%
357
5.0%
6476
90.4%
391
5.5 %
291
4.1 %
42
0.6 %
Parallel – chronic breakdown
13
2241
2.1%
103
4.6%
1960
87.5%
217
9.7 %
177
7.9 %
1
0.0 %
Parallel - Herd or Group Slaughter
3
457
0.4%
36
7.9%
385
84.2%
52
11.4 %
36
7.9 %
0
Parallel - IRs in Exit Strategy for persistent herds
74
268
0.2%
83
31.0%
169
63.1%
17
6.3 %
15
5.6 %
1
0.4 %
Parallel New Infection
403
36238
33.7%
1129
3.1%
31946
88.2%
2929
8.1 %
2354
6.5 %
809
2.2 %
Parallel - new infection cull area
20
3864
3.6%
183
4.7%
3484
90.2%
234
6.1 %
187
4.8 %
10
0.3 %
Parallel - Other
32
1780
1.7%
125
7.0%
1539
86.5%
103
5.8 %
89
5.0 %
27
1.5 %
Parallel Persistent Infection
83
8821
8.2%
461
5.2%
7844
88.9%
571
6.5 %
464
5.3 %
52
0.6 %
Parallel - persistent infection cull area
1
539
0.5%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX anti
27
94
0.1%
11
11.7%
80
85.1%
5
5.3 %
3
3.2 %
0
Parallel - Rapid Testing Two-times IRs
169
412
0.4%
96
23.3%
296
71.8%
23
5.6 %
18
4.4 %
2
0.5 %
Parallel - Recurrent breakdowns
329
44478
41.3%
1949
4.4%
39043
87.8%
3287
7.4 %
2753
6.2 %
733
1.6 %
Parallel - severe IRs - in persistent herds
184
1261
1.2%
365
28.9%
828
65.7%
81
6.4 %
56
4.4 %
12
1.0 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Total
xx-xxxx-xxxx
100.0%
4929
4.6%
94548
87.8%
7944
7.4 %
6470
6.0 %
xx-xxxx-xxxx
1.6 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2023)
England - HRA
Parallel – chronic breakdown
13
2241
2.1%
103
4.6%
1960
87.5%
217
9.7 %
177
7.9 %
1
0.0 %
Parallel - Herd or Group Slaughter
3
457
0.4%
36
7.9%
385
84.2%
52
11.4 %
36
7.9 %
0
Parallel - new infection cull area
18
3664
3.4%
180
4.9%
3294
89.9%
227
6.2 %
180
4.9 %
10
0.3 %
Parallel - Other
9
711
0.7%
51
7.2%
615
86.5%
47
6.6 %
39
5.5 %
6
0.8 %
Parallel Persistent Infection
12
1787
1.7%
127
7.1%
1572
88.0%
106
5.9 %
88
4.9 %
0
Parallel - persistent infection cull area
1
539
0.5%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel - Recurrent breakdowns
234
37476
34.8%
1654
4.4%
32847
87.6%
2762
7.4 %
2310
6.2 %
665
1.8 %
England - Edge
Extended Flexible IFN-GAMMA test
2
55
0.1%
3
5.5%
48
87.3%
4
7.3 %
4
7.3 %
0
Parallel New Infection
98
9913
9.2%
270
2.7%
8955
90.3%
631
6.4 %
514
5.2 %
174
1.8 %
Parallel - new infection cull area
1
191
0.2%
3
1.6%
182
95.3%
6
3.1 %
6
3.1 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel - Other
4
535
0.5%
16
3.0%
489
91.4%
11
2.1 %
11
2.1 %
19
3.6 %
Parallel Persistent Infection
11
2163
2.0%
71
3.3%
1935
89.5%
184
8.5 %
157
7.3 %
0
Parallel - Recurrent breakdowns
35
3355
3.1%
110
3.3%
3022
90.1%
241
7.2 %
208
6.2 %
15
0.4 %
England - LRA
Parallel New Infection
38
4679
4.3%
120
2.6%
3958
84.6%
361
7.7 %
325
6.9 %
276
5.9 %
Parallel Persistent Infection
1
41
0.0%
0
0.0 %
41
100.0%
1
2.4 %
0
0
England
480
67807
63.0%
2775
4.1%
59784
88.2%
4882
7.2 %
4081
6.0 %
1167
1.7 %
Wales - High TB
Anomalous reaction
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
60
6860
6.4%
336
4.9%
6215
90.6%
365
5.3 %
267
3.9 %
42
0.6 %
Parallel - IRs in Exit Strategy for persistent herd
51
211
0.2%
71
33.6%
129
61.1%
10
4.7 %
10
4.7 %
1
0.5 %
Parallel New Infection
2
152
0.1%
13
8.6%
131
86.2%
10
6.6 %
8
5.3 %
0
Parallel - Other
11
258
0.2%
12
4.7%
223
86.4%
27
10.5 %
22
8.5 %
1
0.4 %
Parallel Persistent Infection
48
4349
4.0%
211
4.9%
3908
89.9%
231
5.3 %
178
4.1 %
52
1.2 %
Parallel – post-mvt test coupled with IDEXX ant
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
137
348
0.3%
83
23.9%
248
71.3%
20
5.7 %
15
4.3 %
2
0.6 %
Parallel - Recurrent breakdowns
44
2679
2.5%
151
5.6%
2297
85.7%
220
8.2 %
184
6.9 %
47
1.8 %
Parallel - severe IRs - in persistent herds
156
1071
1.0%
326
30.4%
681
63.6%
77
7.2 %
52
4.9 %
12
1.1 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
86
0.1%
6
7.0%
75
87.2%
5
5.8 %
5
5.8 %
0
Parallel - IRs in Exit Strategy for persistent herd
20
49
0.0%
10
20.4%
34
69.4%
6
12.2 %
5
10.2 %
0
Parallel New Infection
57
4254
4.0%
108
2.5%
3835
90.2%
334
7.9 %
289
6.8 %
22
0.5 %
Parallel - Other
5
263
0.2%
45
17.1%
203
77.2%
15
5.7 %
14
5.3 %
1
0.4 %
Parallel Persistent Infection
10
474
0.4%
52
11.0%
382
80.6%
48
10.1 %
40
8.4 %
0
Parallel – post-mvt test coupled with IDEXX ant
12
65
0.1%
6
9.2%
56
86.2%
4
6.2 %
3
4.6 %
0
Parallel - Rapid Testing Two-times IRs
26
52
0.0%
12
23.1%
38
73.1%
2
3.8 %
2
3.8 %
0
Parallel - Recurrent breakdowns
11
677
0.6%
24
3.5%
610
90.1%
46
6.8 %
38
5.6 %
5
0.7 %
Parallel - severe IRs - in persistent herds
24
183
0.2%
35
19.1%
144
78.7%
4
2.2 %
4
2.2 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
165
0.2%
12
7.3%
138
83.6%
17
10.3 %
15
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
3
8
0.0%
2
25.0%
6
75.0%
1
12.5 %
0
0
Parallel New Infection
146
10214
9.5%
236
2.3%
9063
88.7%
965
9.4 %
652
6.4 %
263
2.6 %
Parallel - Other
2
11
0.0%
1
9.1%
7
63.6%
3
27.3 %
3
27.3 %
0
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX ant
14
28
0.0%
5
17.9%
23
82.1%
1
3.6 %
0
0
Parallel - Rapid Testing Two-times IRs
6
12
0.0%
1
8.3%
10
83.3%
1
8.3 %
1
8.3 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel - Recurrent breakdowns
5
291
0.3%
10
3.4%
267
91.8%
18
6.2 %
13
4.5 %
1
0.3 %
Parallel - severe IRs - in persistent herds
4
7
0.0%
4
57.1%
3
42.9%
0
0
0
Wales
867
32791
30.5%
1772
5.4%
28748
87.7%
2432
7.4 %
1822
5.6 %
449
1.4 %
Scotland - Scotland
Parallel New Infection
62
7026
6.5%
382
5.4%
6004
85.5%
628
8.9 %
566
8.1 %
74
1.1 %
Parallel - new infection cull area
1
9
0.0%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Parallel - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
1
50.0 %
0
0
Scotland
65
7039
6.5%
382
5.4%
6016
85.5%
630
9.0 %
567
8.1 %
74
1.1 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2022 July
248
15402
109
485
4
0
0
0
0
46
1
2
3
0
382
1
46
0
0
August
274
19758
144
758
12
0
0
0
0
36
3
0
14
0
642
0
47
1
2
September
205
xx-xxxx-xxxx
107
672
13
0
0
0
0
100
0
1
7
0
520
0
31
0
0
October
180
xx-xxxx-xxxx
100
620
12
0
0
0
0
66
0
0
21
0
495
0
26
0
0
November
201
15848
120
673
16
0
0
0
0
60
1
1
13
0
504
1
77
0
0
December
200
13735
109
545
36
0
0
0
0
26
1
0
10
0
431
0
41
0
0
2023 January
281
23696
176
1138
10
0
0
0
0
83
3
0
18
0
922
1
100
1
0
February
265
17538
147
1036
6
0
0
0
0
69
1
4
31
0
837
0
88
0
0
March
266
19894
159
867
12
0
0
0
0
76
0
0
11
0
700
1
67
0
0
April
203
16194
118
782
1
14
0
0
0
0
53
0
2
10
5
630
11
48
0
4
May
208
13834
112
582
14
0
0
0
0
43
0
4
0
0
484
37
0
0
0
June
189
xx-xxxx-xxxx
94
524
29
213
0
0
0
0
15
0
7
0
0
188
13
0
0
0
Totals (last
12 months)
2720
205220
1495
8682
30
362
0
0
0
0
673
10
21
138
5
6735
65
571
2
6
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
937
130854
633
4882
253
0
0
0
0
530
1
16
25
3
4008
2
18
0
0
Scotland
91
10443
58
486
3
0
0
0
0
117
2
1
31
0
273
6
21
0
0
Wales
xx-xxxx-xxxx
63923
804
3314
106
0
0
0
0
26
7
4
82
2
2454
57
532
2
6
Totals
2720
205220
1495
8682
362
0
0
0
0
673
10
21
138
5
6735
65
571
2
6
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
2021
2022
2023
IFNg Positive herds
IFNg Negative herds
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
2021
2022
2023
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
2021
2022
2023
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
Wiltshire
Wigtown
Staffs
Somerset excl N
Shropshire
Renfrew
Powys
Perthshire
Pembs
Oxon
Notts
NE Wales
Lincs
Leics & Rut
Kirkcudbright
Heref
Gwynedd
Gwent
Essex
Dorset
Devon
Cumbria
Corn & Scilly
Conwy
Cheshire
Ceredigion
Carms
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during June 2023 (% submission per County)
County
%Resampled %Retested
1.96 %
3.92 %
Avon
Bucks
2.10 %
2.80 %
Carms
4.41 %
5.64 %
Ceredigion
5.08 %
6.78 %
Cheshire
1.98 %
2.97 %
Conwy
12.50 %
12.50 %
Corn & Scilly
9.19 %
11.54 %
Cumbria
14.29 %
14.29 %
Devon
8.28 %
10.21 %
Dorset
1.18 %
2.36 %
Essex
5.77 %
5.77 %
Gwent
2.65 %
3.31 %
Gwynedd
9.16 %
13.99 %
Heref
6.05 %
8.50 %
Kirkcudbright
2.42 %
3.58 %
Leics & Rut
0.97 %
2.42 %
Lincs
NE Wales
5.12 %
6.79 %
Notts
7.14 %
9.13 %
Oxon
3.43 %
5.27 %
Pembs
2.55 %
3.33 %
Perthshire
Powys
3.61 %
4.40 %
Renfrew
50.00 %
Shropshire
2.20 %
2.52 %
Somerset excl
4.80 %
6.83 %
Staffs
12.69 %
20.08 %
Wigtown
15.43 %
19.14 %
Wiltshire
3.88 %
4.92 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
8953
331
3.7%
9076
331
3.6%
Wales
6078
163
2.7%
6123
163
2.7%
Scotland
1282
30
2.3%
1282
30
2.3%
GB
16313
524
3.2%
xx-xxxx-xxxx
524
3.2%
Table 6a (i): Number of animals and submissions tested in June 2023
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
64289
2775
4.3%
67807
2775
4.1%
Wales
30930
1772
5.7%
32791
1772
5.4%
Scotland
4390
382
8.7%
7039
382
5.4%
GB
99591
4929
4.9%
107637
4929
4.6%
Table 6b (i): Number of animals and submissions tested in 2023 Up to end of June
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
13156
348
2.6%
13241
348
2.6%
Wales
4470
242
5.4%
4483
242
5.4%
Scotland
31
0
0.0 %
31
0.0 %
GB
17657
590
3.3%
17755
590
3.3%
Table 6a (ii): Number of animals and submissions tested in June 2022
Table 6b (ii): Number of animals and submissions tested in 2022 Up to end of June
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
85084
3443
4.0%
90839
3443
3.8%
Wales
23465
1656
7.1%
24714
1656
6.7%
Scotland
964
22
2.3%
1025
22
2.1%
GB
109511
5121
4.7%
116578
5121
4.4%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
2
24
0
0
Kent
PRV-SP
1
6
0
0
Northamptonshire
PRV-SE
1
6
3
50.00 %
Total
4
36
3
8.33 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples June 2023
Table 7b Number of private samples 2023
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
4
27
0
0
Cumbria
PRV-SE
1
3
3
100.00 %
Devon
PRV-SE
5
102
10
9.80 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
51
2
3.92 %
Kent
PRV-SP
2
13
0
0
Northamptonshire
PRV-SE
1
6
3
50.00 %
Oxfordshire
PRV-SP
2
2
0
0
Shropshire
PRV-SP
2
2
0
0
West Sussex
PRV-SE
4
553
24
4.34 %
PRV-SP
1
5
0
0
Total
24
766
42
5.48 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
4
14
4
28.6%
Ceredigion
BOV-IR-C
4
53
7
13.2%
BOV-IR-EX
1
12
5
41.7%
North East Wales
BOV-IR-C
3
16
4
25.0%
BOV-IR-EX
1
3
0
0
Pembrokeshire
BOV-IR-C
12
105
18
17.1%
BOV-OTHER
3
50
2
4.0%
BOV-P
1
19
1
5.3%
Powys
BOV-IR-C
4
14
3
21.4%
BOV-IR-EX
1
8
0
0
BOV-OTHER
1
113
5
4.4%
South Wales
BOV-IR-C
1
2
0
0
Wales
36
409
49
12.0%
All GB
36
409
49
12.0 %
Table 8a Number of Cattle Antibody Tests June 2023
Table 8b Number of Cattle Antibody Tests 2023
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
569
38
6.7%
Northern Somerset &
South
Gloucestershire
BOV-P
1
5
0
0
England
3
574
38
6.6%
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
21
162
46
28.4%
BOV-IR-EX
6
36
5
13.9%
BOV-OTHER
1
34
9
26.5%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
18
175
26
14.9%
BOV-IR-EX
3
25
8
32.0%
BOV-OTHER
2
113
3
2.7%
North East Wales
BOV-IR-C
16
137
27
19.7%
BOV-IR-EX
3
14
0
0
BOV-OTHER
1
54
0
0
BOV-P
2
67
2
3.0%
BOV-POSTM
8
21
5
23.8%
Pembrokeshire
BOV-IR-C
82
720
129
17.9%
BOV-IR-EX
17
109
18
16.5%
BOV-OTHER
8
444
20
4.5%
BOV-P
2
47
2
4.3%
Powys
1
8
1
12.5%
BOV-IR-C
24
131
20
15.3%
BOV-IR-EX
1
8
0
0
BOV-OTHER
1
113
5
4.4%
South Wales
BOV-IR-C
6
44
11
25.0%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-IR-EX
2
14
6
42.9%
BOV-OTHER
1
30
1
3.3%
BOV-P
3
186
6
3.2%
Wales
230
2,741
351
12.8%
All GB
234
3,317
390
11.8 %