Monthly report: Survellience Project SB4008 IFNg tests for Bovine tuberculosis - August 2019
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 156
Report Period 1st - 31st August 2019
Issued 18 September 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
August 2019
%
Total 2019
%
Submissions
262
2422
Samples
Total (%)
21887
100.00 %
224581
100.00 %
England
18297
83.60 %
179993
80.15 %
Wales
3236
14.79 %
42378
18.87 %
Scotland
354
1.62 %
2210
0.98 %
Parallel Tests
PAR-CUL-N
4947
22.60 %
47094
20.97 %
PAR-CUL-P
2479
11.33 %
13972
6.22 %
PAR-HERD-S
358
1.64 %
4435
1.97 %
PAR-NEW-IN
8098
37.00 %
103483
46.08 %
PAR-OTHER
226
1.03 %
3959
1.76 %
PAR-PERSIS
5286
24.15 %
45324
20.18 %
PAR-RAPID
77
0.35 %
886
0.39 %
Total(% of all samples)
21471
98.10 %
219153
97.58 %
Serial/Extended Tests
SER-FLEXI
416
1.90 %
5402
2.41 %
SER-FRAUD
0
0.00 %
18
0.01 %
SER-NSR
0
0.00 %
8
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
416
1.90 %
5428
2.42 %
Total
21887
100.00 %
224581
100.00 %
Retests
Total (% of all samples)
2044
9.34 %
15941
7.10 %
England
1837
8.39 %
13209
5.88 %
Wales
195
0.89 %
2632
1.17 %
Scotland
12
0.05 %
100
0.04 %
Resamples
Total (% of all samples)
1513
6.91 %
12475
5.55 %
England
1393
6.36 %
10356
4.61 %
Wales
111
0.51 %
2037
0.91 %
Scotland
9
0.04 %
82
0.04 %
Rejects
Total (% of all samples)
96
0.44 %
1983
0.88 %
England
93
0.42 %
1802
0.80 %
Wales
3
0.01 %
181
0.08 %
Scotland
0
0.00 %
Table 1. Monthly Totals for August 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
4947
22.6 %
PAR-CUL-P
2479
11.3 %
PAR-HERD-S
358
1.6 %
PAR-NEW-IN
8098
37.0 %
PAR-OTHER
226
1.0 %
PAR-PERSIS
5286
24.2 %
PAR-RAPID
77
0.4 %
SER-FLEXI
416
1.9 %
Total
21887
100.00 %
Figure 1. Number of samples submitted in each category during August 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
Kirkcudbright
Dumfries & G
W Glamorgan
Powys
Pembs
NE Wales
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northumb
Northants
Leics & Rut
Heref
Hampshire
Gr London
Gloucs
Gloucestershire
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during August 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
Wigtown
Kirkcudbright
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Wiltshire
Warks
S Yorks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Lincs
Leics & Rut
Leicestershire and Rutland
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gr London
Gloucs
Gloucestershire
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Avon
cotland
Wales
ngland
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
2
60
0.27 %
0
0.00 %
56
93.33 %
4
6.67 %
4
6.67 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
2
132
0.60 %
6
4.55 %
112
84.85 %
17
12.88 %
14
10.61 %
0
0.00 %
PAR-NEW-IN
2
231
1.06 %
5
2.16 %
186
80.52 %
52
22.51 %
40
17.32 %
0
0.00 %
PAR-PERSIS
5
1,986
9.07 %
23
1.16 %
1865
93.91 %
130
6.55 %
98
4.93 %
0
0.00 %
Devon
PAR-CUL-N
24
2,410
11.01 %
98
4.07 %
2133
88.51 %
231
9.59 %
179
7.43 %
0
0.00 %
PAR-CUL-P
2
572
2.61 %
3
0.52 %
518
90.56 %
75
13.11 %
51
8.92 %
0
0.00 %
Dorset
PAR-CUL-N
8
813
3.71 %
64
7.87 %
694
85.36 %
79
9.72 %
55
6.77 %
0
0.00 %
PAR-CUL-P
3
1,217
5.56 %
97
7.97 %
1087
89.32 %
65
5.34 %
33
2.71 %
0
0.00 %
Gloucestershire
PAR-NEW-IN
1
112
0.51 %
3
2.68 %
103
91.96 %
7
6.25 %
6
5.36 %
0
0.00 %
Gloucs
PAR-CUL-N
1
147
0.67 %
0
0.00 %
89
60.54 %
58
39.46 %
58
39.46 %
0
0.00 %
PAR-CUL-P
2
83
0.38 %
2
2.41 %
53
63.86 %
32
38.55 %
28
33.73 %
0
0.00 %
Heref
PAR-CUL-N
1
171
0.78 %
2
1.17 %
166
97.08 %
4
2.34 %
3
1.75 %
0
0.00 %
PAR-PERSIS
2
129
0.59 %
2
1.55 %
121
93.80 %
7
5.43 %
6
4.65 %
0
0.00 %
Shropshire
PAR-PERSIS
3
219
1.00 %
7
3.20 %
173
79.00 %
45
20.55 %
39
17.81 %
0
0.00 %
Somerset excl N
PAR-CUL-N
5
688
3.14 %
17
2.47 %
644
93.60 %
40
5.81 %
27
3.92 %
0
0.00 %
Staffs
PAR-OTHER
1
128
0.58 %
3
2.34 %
113
88.28 %
14
10.94 %
12
9.38 %
0
0.00 %
Wiltshire
PAR-CUL-N
6
586
2.68 %
14
2.39 %
528
90.10 %
49
8.36 %
44
7.51 %
0
0.00 %
PAR-CUL-P
4
607
2.77 %
17
2.80 %
549
90.44 %
58
9.56 %
41
6.75 %
0
0.00 %
PAR-NEW-IN
2
18
0.08 %
0
0.00 %
15
83.33 %
3
16.67 %
3
16.67 %
0
0.00 %
PAR-PERSIS
2
42
0.19 %
0
0.00 %
31
73.81 %
17
40.48 %
11
26.19 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
3
130
0.59 %
1
0.77 %
116
89.23 %
14
10.77 %
13
10.00 %
0
0.00 %
Bucks
PAR-NEW-IN
4
186
0.85 %
12
6.45 %
136
73.12 %
42
22.58 %
38
20.43 %
0
0.00 %
Cheshire
PAR-NEW-IN
14
2,862
13.08 %
51
1.78 %
2666
93.15 %
227
7.93 %
145
5.07 %
0
0.00 %
Derbyshire
PAR-NEW-IN
10
1,337
6.11 %
31
2.32 %
1216
90.95 %
128
9.57 %
90
6.73 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
725
3.31 %
15
2.07 %
640
88.28 %
100
13.79 %
72
9.93 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
1
17
0.08 %
0
0.00 %
16
94.12 %
3
17.65 %
1
5.88 %
0
0.00 %
Table 2a. Summary by County for August 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for August 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Northants
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
11
835
3.82 %
23
2.75 %
734
87.90 %
100
11.98 %
78
9.34 %
0
0.00 %
Warks
PAR-NEW-IN
5
813
3.71 %
12
1.48 %
761
93.60 %
49
6.03 %
40
4.92 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
9
0.04 %
0
0.00 %
6
66.67 %
4
44.44 %
3
33.33 %
0
0.00 %
Gr London
PAR-NEW-IN
1
43
0.20 %
0
0.00 %
41
95.35 %
4
9.30 %
2
4.65 %
0
0.00 %
Northumb
PAR-NEW-IN
2
205
0.94 %
0
0.00 %
153
74.63 %
60
29.27 %
52
25.37 %
0
0.00 %
PAR-PERSIS
3
183
0.84 %
1
0.55 %
32
17.49 %
58
31.69 %
57
31.15 %
93
50.82 %
N Yorks
PAR-PERSIS
2
553
2.53 %
2
0.36 %
529
95.66 %
31
5.61 %
22
3.98 %
0
0.00 %
W Sussex
PAR-NEW-IN
1
46
0.21 %
0
0.00 %
18
39.13 %
30
65.22 %
28
60.87 %
0
0.00 %
England
143
18297
83.60 %
511
2.79 %
16302
89.10 %
1837
10.04 %
1393
7.61 %
93
0.51 %
Wales - High TB
Carms
PAR-HERD-S
5
173
0.79 %
14
8.09 %
153
88.44 %
12
6.94 %
6
3.47 %
0
0.00 %
PAR-OTHER
2
8
0.04 %
5
62.50 %
3
37.50 %
1
12.50 %
0
0.00 %
0
0.00 %
PAR-PERSIS
7
576
2.63 %
45
7.81 %
516
89.58 %
19
3.30 %
15
2.60 %
0
0.00 %
PAR-RAPID
6
34
0.16 %
7
20.59 %
25
73.53 %
3
8.82 %
2
5.88 %
0
0.00 %
SER-FLEXI
7
104
0.48 %
14
13.46 %
85
81.73 %
7
6.73 %
5
4.81 %
0
0.00 %
Ceredigion
PAR-OTHER
1
7
0.03 %
0
0.00 %
7
100.00 %
1
14.29 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
392
1.79 %
10
2.55 %
367
93.62 %
24
6.12 %
15
3.83 %
0
0.00 %
PAR-RAPID
2
8
0.04 %
1
12.50 %
7
87.50 %
1
12.50 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
42
0.19 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Gwent
PAR-PERSIS
2
4
0.02 %
1
25.00 %
1
25.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RAPID
2
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
4
184
0.84 %
10
5.43 %
172
93.48 %
16
8.70 %
2
1.09 %
0
0.00 %
PAR-NEW-IN
3
8
0.04 %
2
25.00 %
4
50.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-PERSIS
16
628
2.87 %
32
5.10 %
570
90.76 %
35
5.57 %
26
4.14 %
0
0.00 %
PAR-RAPID
7
9
0.04 %
3
33.33 %
5
55.56 %
0
0.00 %
0
0.00 %
1
11.11 %
SER-FLEXI
7
157
0.72 %
8
5.10 %
143
91.08 %
7
4.46 %
5
3.18 %
1
0.64 %
Powys
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
204
0.93 %
11
5.39 %
186
91.18 %
12
5.88 %
6
2.94 %
1
0.49 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for August 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RAPID
7
13
0.06 %
3
23.08 %
10
76.92 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
12
0.05 %
0
0.00 %
11
91.67 %
1
8.33 %
1
8.33 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
3
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
5
247
1.13 %
22
8.91 %
220
89.07 %
17
6.88 %
5
2.02 %
0
0.00 %
PAR-OTHER
2
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
PAR-PERSIS
1
234
1.07 %
11
4.70 %
217
92.74 %
16
6.84 %
6
2.56 %
0
0.00 %
SER-FLEXI
5
99
0.45 %
5
5.05 %
90
90.91 %
6
6.06 %
4
4.04 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
NE Wales
PAR-NEW-IN
1
52
0.24 %
2
3.85 %
49
94.23 %
4
7.69 %
1
1.92 %
0
0.00 %
PAR-OTHER
2
18
0.08 %
1
5.56 %
15
83.33 %
3
16.67 %
2
11.11 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Wales
117
3236
14.79 %
211
6.52 %
2911
89.96 %
195
6.03 %
111
3.43 %
3
0.09 %
Scotland - Scotland
Dumfries & G
PAR-NEW-IN
1
16
0.07 %
0
0.00 %
14
87.50 %
5
31.25 %
2
12.50 %
0
0.00 %
Kirkcudbright
PAR-PERSIS
1
338
1.54 %
2
0.59 %
329
97.34 %
7
2.07 %
7
2.07 %
0
0.00 %
Scotland
2
354
1.62 %
2
0.56 %
343
96.89 %
12
3.39 %
9
2.54 %
0
0.00 %
Grand Total
Sum:
262
21887
724
19556
2044
1513
96
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
3
431
0.19 %
9
2.09 %
407
94.43 %
23
5.34 %
15
3.48 %
0
0.00 %
PAR-OTHER
3
81
0.04 %
1
1.23 %
69
85.19 %
11
13.58 %
11
13.58 %
0
0.00 %
PAR-PERSIS
9
1,490
0.66 %
45
3.02 %
1387
93.09 %
72
4.83 %
56
3.76 %
2
0.13 %
SER-FRAUD
2
18
0.01 %
0
0.00 %
17
94.44 %
1
5.56 %
1
5.56 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
38
4,045
1.80 %
127
3.14 %
3608
89.20 %
318
7.86 %
259
6.40 %
51
1.26 %
PAR-CUL-P
4
1,165
0.52 %
19
1.63 %
1035
88.84 %
132
11.33 %
109
9.36 %
2
0.17 %
PAR-NEW-IN
4
355
0.16 %
11
3.10 %
287
80.85 %
70
19.72 %
57
16.06 %
0
0.00 %
PAR-PERSIS
29
7,089
3.16 %
252
3.55 %
6245
88.09 %
398
5.61 %
297
4.19 %
295
4.16 %
SER-FLEXI
2
15
0.01 %
0
0.00 %
15
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Devon
PAR-CUL-N
161
20,682
9.21 %
1154
5.58 %
18427
89.10 %
1322
6.39 %
994
4.81 %
107
0.52 %
PAR-CUL-P
22
5,097
2.27 %
289
5.67 %
4498
88.25 %
376
7.38 %
306
6.00 %
4
0.08 %
PAR-NEW-IN
4
674
0.30 %
106
15.73 %
501
74.33 %
86
12.76 %
67
9.94 %
0
0.00 %
PAR-OTHER
7
532
0.24 %
6
1.13 %
352
66.17 %
202
37.97 %
174
32.71 %
0
0.00 %
PAR-PERSIS
19
2,346
1.04 %
162
6.91 %
2038
86.87 %
190
8.10 %
145
6.18 %
1
0.04 %
SER-FLEXI
8
849
0.38 %
50
5.89 %
732
86.22 %
47
5.54 %
22
2.59 %
45
5.30 %
Dorset
PAR-CUL-N
79
9,752
4.34 %
546
5.60 %
8650
88.70 %
645
6.61 %
500
5.13 %
56
0.57 %
PAR-CUL-P
13
2,896
1.29 %
234
8.08 %
2562
88.47 %
140
4.83 %
100
3.45 %
0
0.00 %
PAR-NEW-IN
2
36
0.02 %
1
2.78 %
27
75.00 %
8
22.22 %
8
22.22 %
0
0.00 %
SER-FLEXI
4
153
0.07 %
6
3.92 %
137
89.54 %
11
7.19 %
10
6.54 %
0
0.00 %
Gloucestershire
PAR-NEW-IN
1
112
0.05 %
3
2.68 %
103
91.96 %
7
6.25 %
6
5.36 %
0
0.00 %
Gloucs
PAR-CUL-N
43
2,996
1.33 %
119
3.97 %
2508
83.71 %
314
10.48 %
261
8.71 %
108
3.60 %
PAR-CUL-P
8
1,195
0.53 %
46
3.85 %
1040
87.03 %
132
11.05 %
109
9.12 %
0
0.00 %
PAR-HERD-S
4
33
0.01 %
0
0.00 %
31
93.94 %
3
9.09 %
2
6.06 %
0
0.00 %
SER-FLEXI
2
30
0.01 %
2
6.67 %
28
93.33 %
2
6.67 %
0
0.00 %
0
0.00 %
Heref
PAR-CUL-N
15
1,724
0.77 %
69
4.00 %
1541
89.39 %
129
7.48 %
103
5.97 %
11
0.64 %
PAR-CUL-P
2
182
0.08 %
4
2.20 %
159
87.36 %
22
12.09 %
19
10.44 %
0
0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
1
11
0.00 %
0
0.00 %
10
90.91 %
4
36.36 %
1
9.09 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
25
3,296
1.47 %
128
3.88 %
2970
90.11 %
242
7.34 %
192
5.83 %
6
0.18 %
Shropshire
PAR-CUL-P
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
525
0.23 %
38
7.24 %
448
85.33 %
45
8.57 %
38
7.24 %
1
0.19 %
PAR-PERSIS
10
935
0.42 %
26
2.78 %
809
86.52 %
111
11.87 %
99
10.59 %
1
0.11 %
SER-FLEXI
5
557
0.25 %
21
3.77 %
509
91.38 %
34
6.10 %
27
4.85 %
0
0.00 %
Somerset excl N
PAR-CUL-N
29
3,166
1.41 %
161
5.09 %
2865
90.49 %
190
6.00 %
138
4.36 %
2
0.06 %
PAR-OTHER
2
238
0.11 %
34
14.29 %
155
65.13 %
8
3.36 %
8
3.36 %
41
17.23 %
PAR-PERSIS
13
1,907
0.85 %
110
5.77 %
1510
79.18 %
105
5.51 %
69
3.62 %
218
11.43 %
Staffs
PAR-OTHER
1
128
0.06 %
3
2.34 %
113
88.28 %
14
10.94 %
12
9.38 %
0
0.00 %
PAR-PERSIS
7
1,492
0.66 %
202
13.54 %
1201
80.50 %
130
8.71 %
90
6.03 %
0
0.00 %
Wiltshire
PAR-CUL-N
47
4,469
1.99 %
188
4.21 %
3994
89.37 %
310
6.94 %
258
5.77 %
29
0.65 %
PAR-CUL-P
15
2,997
1.33 %
176
5.87 %
2690
89.76 %
182
6.07 %
130
4.34 %
1
0.03 %
PAR-NEW-IN
6
454
0.20 %
15
3.30 %
396
87.22 %
53
11.67 %
43
9.47 %
0
0.00 %
PAR-OTHER
2
37
0.02 %
1
2.70 %
34
91.89 %
4
10.81 %
2
5.41 %
0
0.00 %
PAR-PERSIS
6
1,188
0.53 %
25
2.10 %
1108
93.27 %
72
6.06 %
50
4.21 %
5
0.42 %
SER-FLEXI
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
37
4,288
1.91 %
154
3.59 %
3847
89.72 %
351
8.19 %
287
6.69 %
0
0.00 %
PAR-PERSIS
3
204
0.09 %
5
2.45 %
196
96.08 %
5
2.45 %
3
1.47 %
0
0.00 %
Bucks
PAR-NEW-IN
25
2,403
1.07 %
85
3.54 %
2100
87.39 %
249
10.36 %
203
8.45 %
15
0.62 %
Cheshire
PAR-CUL-N
1
260
0.12 %
19
7.31 %
229
88.08 %
12
4.62 %
12
4.62 %
0
0.00 %
PAR-NEW-IN
160
32,187
14.33 %
810
2.52 %
29798
92.58 %
1956
6.08 %
1428
4.44 %
151
0.47 %
PAR-PERSIS
5
540
0.24 %
3
0.56 %
512
94.81 %
29
5.37 %
25
4.63 %
0
0.00 %
Derbyshire
PAR-NEW-IN
127
13,956
6.21 %
476
3.41 %
12299
88.13 %
1153
8.26 %
968
6.94 %
213
1.53 %
E Sussex
PAR-NEW-IN
21
2,721
1.21 %
125
4.59 %
2450
90.04 %
157
5.77 %
149
5.48 %
13
0.48 %
Hampshire
PAR-NEW-IN
31
2,678
1.19 %
68
2.54 %
2428
90.66 %
231
8.63 %
184
6.87 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-NSR
2
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leicestershire and
Rutland
PAR-NEW-IN
1
24
0.01 %
0
0.00 %
24
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
52
6,250
2.78 %
261
4.18 %
5544
88.70 %
433
6.93 %
350
5.60 %
95
1.52 %
Northants
PAR-NEW-IN
27
1,888
0.84 %
117
6.20 %
xx-xxxx-xxxx
89.41 %
129
6.83 %
83
4.40 %
0
0.00 %
Notts
PAR-NEW-IN
7
774
0.34 %
36
4.65 %
618
79.84 %
69
8.91 %
56
7.24 %
64
8.27 %
Oxon
PAR-NEW-IN
107
9,900
4.41 %
428
4.32 %
8750
88.38 %
852
8.61 %
672
6.79 %
50
0.51 %
Warks
PAR-NEW-IN
79
6,976
3.11 %
321
4.60 %
6149
88.15 %
533
7.64 %
422
6.05 %
84
1.20 %
PAR-PERSIS
6
570
0.25 %
19
3.33 %
526
92.28 %
32
5.61 %
25
4.39 %
0
0.00 %
England - LRA
Cambs
PAR-NEW-IN
6
398
0.18 %
22
5.53 %
347
87.19 %
27
6.78 %
21
5.28 %
8
2.01 %
Cumbria
PAR-NEW-IN
16
3,306
1.47 %
110
3.33 %
3007
90.96 %
254
7.68 %
189
5.72 %
0
0.00 %
Essex
PAR-NEW-IN
2
94
0.04 %
1
1.06 %
91
96.81 %
2
2.13 %
2
2.13 %
0
0.00 %
Gr London
PAR-NEW-IN
1
43
0.02 %
0
0.00 %
41
95.35 %
4
9.30 %
2
4.65 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
2
79
0.04 %
6
7.59 %
52
65.82 %
25
31.65 %
21
26.58 %
0
0.00 %
Herts
PAR-NEW-IN
7
1,011
0.45 %
49
4.85 %
886
87.64 %
90
8.90 %
76
7.52 %
0
0.00 %
I of Wight
PAR-NEW-IN
4
641
0.29 %
16
2.50 %
562
87.68 %
69
10.76 %
62
9.67 %
1
0.16 %
Kent
PAR-NEW-IN
1
10
0.00 %
1
10.00 %
9
90.00 %
3
30.00 %
0
0.00 %
0
0.00 %
Lancashire
PAR-NEW-IN
3
106
0.05 %
1
0.94 %
101
95.28 %
5
4.72 %
4
3.77 %
0
0.00 %
Lincs
PAR-NEW-IN
8
754
0.34 %
20
2.65 %
693
91.91 %
46
6.10 %
41
5.44 %
0
0.00 %
Northumb
PAR-NEW-IN
2
205
0.09 %
0
0.00 %
153
74.63 %
60
29.27 %
52
25.37 %
0
0.00 %
PAR-PERSIS
3
183
0.08 %
1
0.55 %
32
17.49 %
58
31.69 %
57
31.15 %
93
50.82 %
N Yorks
PAR-NEW-IN
7
875
0.39 %
22
2.51 %
766
87.54 %
71
8.11 %
59
6.74 %
28
3.20 %
PAR-PERSIS
2
553
0.25 %
2
0.36 %
529
95.66 %
31
5.61 %
22
3.98 %
0
0.00 %
SER-FLEXI
2
50
0.02 %
1
2.00 %
48
96.00 %
3
6.00 %
1
2.00 %
0
0.00 %
S Yorks
PAR-NEW-IN
5
404
0.18 %
7
1.73 %
381
94.31 %
21
5.20 %
15
3.71 %
1
0.25 %
W Sussex
PAR-NEW-IN
2
239
0.11 %
2
0.84 %
163
68.20 %
81
33.89 %
74
30.96 %
0
0.00 %
W Yorks
PAR-HERD-S
2
24
0.01 %
8
33.33 %
14
58.33 %
2
8.33 %
2
8.33 %
0
0.00 %
England
1431
179993
80.15 %
7586
4.21 %
160268
89.04 %
13209
7.34 %
10356
5.75 %
1802
1.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - High TB
Carms
PAR-HERD-S
48
2,494
1.11 %
166
6.66 %
2193
87.93 %
169
6.78 %
133
5.33 %
2
0.08 %
PAR-NEW-IN
3
121
0.05 %
22
18.18 %
90
74.38 %
11
9.09 %
9
7.44 %
0
0.00 %
PAR-OTHER
13
437
0.19 %
58
13.27 %
372
85.13 %
17
3.89 %
7
1.60 %
0
0.00 %
PAR-PERSIS
59
5,819
2.59 %
299
5.14 %
5283
90.79 %
303
5.21 %
226
3.88 %
13
0.22 %
PAR-RAPID
59
250
0.11 %
67
26.80 %
169
67.60 %
23
9.20 %
14
5.60 %
0
0.00 %
SER-FLEXI
35
834
0.37 %
87
10.43 %
707
84.77 %
49
5.88 %
40
4.80 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
143
0.06 %
12
8.39 %
119
83.22 %
15
10.49 %
12
8.39 %
0
0.00 %
PAR-NEW-IN
2
313
0.14 %
14
4.47 %
289
92.33 %
10
3.19 %
10
3.19 %
0
0.00 %
PAR-OTHER
3
160
0.07 %
5
3.13 %
148
92.50 %
10
6.25 %
7
4.38 %
0
0.00 %
PAR-PERSIS
22
1,900
0.85 %
129
6.79 %
1669
87.84 %
137
7.21 %
102
5.37 %
0
0.00 %
PAR-RAPID
35
92
0.04 %
22
23.91 %
66
71.74 %
6
6.52 %
4
4.35 %
0
0.00 %
SER-FLEXI
5
493
0.22 %
18
3.65 %
462
93.71 %
13
2.64 %
11
2.23 %
2
0.41 %
Gwent
PAR-HERD-S
11
887
0.39 %
50
5.64 %
760
85.68 %
84
9.47 %
75
8.46 %
2
0.23 %
PAR-OTHER
1
15
0.01 %
3
20.00 %
12
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
16
1,102
0.49 %
57
5.17 %
989
89.75 %
73
6.62 %
55
4.99 %
1
0.09 %
PAR-RAPID
24
69
0.03 %
15
21.74 %
49
71.01 %
5
7.25 %
4
5.80 %
1
1.45 %
SER-FLEXI
1
20
0.01 %
2
10.00 %
18
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
25
429
0.19 %
43
10.02 %
366
85.31 %
41
9.56 %
21
4.90 %
0
0.00 %
PAR-NEW-IN
3
8
0.00 %
2
25.00 %
4
50.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-OTHER
14
1,213
0.54 %
55
4.53 %
1073
88.46 %
106
8.74 %
85
7.01 %
0
0.00 %
PAR-PERSIS
105
10,699
4.76 %
337
3.15 %
9815
91.74 %
677
6.33 %
543
5.08 %
4
0.04 %
PAR-RAPID
92
229
0.10 %
43
18.78 %
174
75.98 %
17
7.42 %
11
4.80 %
1
0.44 %
SER-FLEXI
38
936
0.42 %
61
6.52 %
839
89.64 %
50
5.34 %
35
3.74 %
1
0.11 %
Powys
PAR-HERD-S
17
203
0.09 %
49
24.14 %
139
68.47 %
13
6.40 %
11
5.42 %
4
1.97 %
PAR-NEW-IN
21
567
0.25 %
18
3.17 %
522
92.06 %
32
5.64 %
26
4.59 %
1
0.18 %
PAR-OTHER
1
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
29
1,272
0.57 %
56
4.40 %
1136
89.31 %
93
7.31 %
77
6.05 %
3
0.24 %
PAR-RAPID
51
125
0.06 %
17
13.60 %
77
61.60 %
6
4.80 %
6
4.80 %
25
20.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
11
342
0.15 %
21
6.14 %
316
92.40 %
6
1.75 %
5
1.46 %
0
0.00 %
S Wales
PAR-HERD-S
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
11
0.00 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
384
0.17 %
5
1.30 %
371
96.61 %
8
2.08 %
8
2.08 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
101
0.04 %
5
4.95 %
92
91.09 %
5
4.95 %
4
3.96 %
0
0.00 %
W Glamorgan
PAR-HERD-S
3
118
0.05 %
7
5.93 %
109
92.37 %
5
4.24 %
2
1.69 %
0
0.00 %
PAR-PERSIS
1
7
0.00 %
0
0.00 %
5
71.43 %
2
28.57 %
2
28.57 %
0
0.00 %
PAR-RAPID
3
6
0.00 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
75
0.03 %
14
18.67 %
59
78.67 %
3
4.00 %
2
2.67 %
0
0.00 %
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
6
351
0.16 %
14
3.99 %
324
92.31 %
16
4.56 %
13
3.70 %
0
0.00 %
PAR-PERSIS
7
560
0.25 %
29
5.18 %
516
92.14 %
27
4.82 %
15
2.68 %
0
0.00 %
PAR-RAPID
10
21
0.01 %
3
14.29 %
17
80.95 %
1
4.76 %
1
4.76 %
0
0.00 %
SER-FLEXI
1
20
0.01 %
12
60.00 %
8
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
15
0.01 %
0
0.00 %
6
40.00 %
9
60.00 %
9
60.00 %
0
0.00 %
PAR-OTHER
2
148
0.07 %
21
14.19 %
111
75.00 %
17
11.49 %
16
10.81 %
0
0.00 %
PAR-PERSIS
4
550
0.24 %
26
4.73 %
500
90.91 %
28
5.09 %
24
4.36 %
0
0.00 %
PAR-RAPID
3
5
0.00 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
27
0.01 %
2
7.41 %
21
77.78 %
4
14.81 %
4
14.81 %
0
0.00 %
NE Wales
PAR-NEW-IN
64
5,206
2.32 %
174
3.34 %
4730
90.86 %
331
6.36 %
250
4.80 %
52
1.00 %
PAR-OTHER
4
58
0.03 %
16
27.59 %
40
68.97 %
4
6.90 %
2
3.45 %
0
0.00 %
PAR-PERSIS
3
471
0.21 %
19
4.03 %
438
92.99 %
30
6.37 %
14
2.97 %
0
0.00 %
PAR-RAPID
12
42
0.02 %
8
19.05 %
33
78.57 %
1
2.38 %
1
2.38 %
0
0.00 %
SER-FLEXI
31
825
0.37 %
65
7.88 %
670
81.21 %
45
5.45 %
37
4.48 %
53
6.42 %
Powys
PAR-NEW-IN
10
809
0.36 %
25
3.09 %
727
89.86 %
64
7.91 %
57
7.05 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
7
34
0.02 %
3
8.82 %
31
91.18 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2019 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
3
59
0.03 %
10
xx-xxxx-xxxx %
48
81.36 %
2
3.39 %
1
1.69 %
0
0.00 %
S Wales
PAR-RAPID
2
4
0.00 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
W Glamorgan
PAR-HERD-S
2
11
0.00 %
3
27.27 %
7
63.64 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
9
272
0.12 %
10
3.68 %
254
93.38 %
10
3.68 %
7
2.57 %
1
0.37 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
13
459
0.20 %
8
1.74 %
420
91.50 %
25
5.45 %
16
3.49 %
15
3.27 %
PAR-OTHER
2
18
0.01 %
1
5.56 %
15
83.33 %
3
16.67 %
2
11.11 %
0
0.00 %
PAR-PERSIS
2
429
0.19 %
7
1.63 %
409
95.34 %
17
3.96 %
13
3.03 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
89
0.04 %
4
4.49 %
82
92.13 %
4
4.49 %
3
3.37 %
0
0.00 %
Wales
976
42378
18.87 %
2225
5.25 %
37938
89.52 %
2632
6.21 %
2037
4.81 %
181
0.43 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
1
39
0.02 %
1
2.56 %
38
97.44 %
0
0.00 %
0
0.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
366
0.16 %
4
1.09 %
345
94.26 %
20
5.46 %
17
4.64 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
2
643
0.29 %
10
1.56 %
615
95.65 %
23
3.58 %
18
2.80 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
3
404
0.18 %
55
13.61 %
337
83.42 %
14
3.47 %
12
2.97 %
0
0.00 %
PAR-PERSIS
1
338
0.15 %
2
0.59 %
329
97.34 %
7
2.07 %
7
2.07 %
0
0.00 %
Wigtown
PAR-NEW-IN
5
420
0.19 %
11
2.62 %
381
90.71 %
36
8.57 %
28
6.67 %
0
0.00 %
Scotland
15
2210
0.98 %
83
3.76 %
2045
92.53 %
100
4.52 %
82
3.71 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
9894
200251
15941
12475
1983
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol August 2019
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
143
18297
100.0%
511
2.8%
16302
89.1%
1837
10.0%
1393
7.6%
93
0.5%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
143
18297
100.0%
511
2.8%
16302
89.1%
1837
10.0%
1393
7.6%
93
0.5%
Scotland
Parallel
2
354
100.0%
2
0.6%
343
96.9%
12
3.4%
9
2.5%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
354
100.0%
2
0.6%
343
96.9%
12
3.4%
9
2.5%
0
0.0 %
Wales
Parallel
93
2820
87.1%
183
6.5%
2543
90.2%
170
6.0%
92
3.3%
2
0.1%
Serial/Extended
24
416
12.9%
28
6.7%
368
88.5%
25
6.0%
19
4.6%
1
0.2%
Total
117
3236
100.0%
211
6.5%
2911
90.0%
195
6.0%
111
3.4%
3
0.1%
GB
Parallel
238
21471
98.1%
696
3.2%
19188
89.4%
2019
9.4%
1494
7.0%
95
0.4%
Serial/Extended
24
416
1.9%
28
6.7%
368
88.5%
25
6.0%
19
4.6%
1
0.2%
Total
262
21887
100.0%
724
3.3%
19556
89.3%
2044
9.3%
1513
6.9%
96
0.4%
143.00
117.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.1%
7506
4.2% 158773
89.0%
13110
7.4%
10294
5.8%
1757
1.0%
Serial/Extended
28
xx-xxxx-xxxx
0.9%
80
4.8%
1495
88.9%
99
5.9%
62
3.7%
45
2.7%
Total
xx-xxxx-xxxx
100.0%
7586
4.2% 160268
89.0%
13209
7.3%
10356
5.8%
1802
1.0%
Scotland
Parallel
15
2210
100.0%
83
3.8%
2045
92.5%
100
4.5%
82
3.7%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
15
2210
100.0%
83
3.8%
2045
92.5%
100
4.5%
82
3.7%
0
0.0 %
Wales
Parallel
844
38632
91.2%
1938
5.0%
34675
89.8%
2454
6.4%
1897
4.9%
125
0.3%
Serial/Extended
132
3746
8.8%
287
7.7%
3263
87.1%
178
4.8%
140
3.7%
56
1.5%
Total
976
42378
100.0%
2225
5.3%
37938
89.5%
2632
6.2%
2037
4.8%
181
0.4%
GB
Parallel
xx-xxxx-xxxx
97.6%
9527
4.3% 195493
89.2%
15664
7.1%
12273
5.6%
1882
0.9%
Serial/Extended
160
5428
2.4%
367
6.8%
4758
87.7%
277
5.1%
202
3.7%
101
1.9%
Total
xx-xxxx-xxxx
100.0%
9894
4.4% 200251
89.2%
15941
7.1%
12475
5.6%
1983
0.9%
#######
976.00
15.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
24
416
1.9%
28
6.7%
368
88.5%
25
6.0 %
19
4.6 %
1
0.2 %
Parallel - Herd or Group Slaughter
10
358
1.6%
24
6.7%
326
91.1%
28
7.8 %
8
2.2 %
0
Parallel New Infection
81
8098
37.0%
190
2.3%
7282
89.9%
863
10.7 %
627
7.7 %
1
0.0 %
Parallel - new infection cull area
47
4947
22.6%
201
4.1%
4366
88.3%
478
9.7 %
380
7.7 %
0
Parallel - Other
11
226
1.0%
9
4.0%
198
87.6%
24
10.6 %
19
8.4 %
0
Parallel Persistent Infection
49
5286
24.2%
136
2.6%
4752
89.9%
392
7.4 %
305
5.8 %
93
1.8 %
Parallel - persistent infection cull area
11
2479
11.3%
119
4.8%
2207
89.0%
230
9.3 %
153
6.2 %
0
Parallel - Rapid Testing Two-times IRs
29
77
0.4%
17
22.1%
57
74.0%
4
5.2 %
2
2.6 %
1
1.3 %
Total
262
21887
100.0%
724
3.3%
19556
89.3%
2044
9.3 %
1513
6.9 %
96
0.4 %
Table 4a. Summaryof IFN Gamma results by submission reason (August 2019)
England - HRA
Parallel New Infection
5
361
1.6%
8
2.2%
304
84.2%
62
17.2 %
49
13.6 %
0
Parallel - new infection cull area
47
4947
22.6%
201
4.1%
4366
88.3%
478
9.7 %
380
7.7 %
0
Parallel - Other
3
188
0.9%
3
1.6%
169
89.9%
18
9.6 %
16
8.5 %
0
Parallel Persistent Infection
12
2376
10.9%
32
1.3%
2190
92.2%
199
8.4 %
154
6.5 %
0
Parallel - persistent infection cull area
11
2479
11.3%
119
4.8%
2207
89.0%
230
9.3 %
153
6.2 %
0
England - HRA
78
10351
47.3%
363
3.5%
9236
89.2%
987
9.5 %
752
7.3 %
0
England - Edge
Parallel New Infection
55
6907
31.6%
145
2.1%
6287
91.0%
663
9.6 %
477
6.9 %
0
England - Edge
55
6907
31.6%
145
2.1%
6287
91.0%
663
9.6 %
477
6.9 %
0
England - LRA
Parallel New Infection
5
303
1.4%
0
0.0 %
218
71.9%
98
32.3 %
85
28.1 %
0
Parallel Persistent Infection
5
736
3.4%
3
0.4%
561
76.2%
89
12.1 %
79
10.7 %
93
12.6 %
England - LRA
10
1039
4.7%
3
0.3%
779
75.0%
187
18.0 %
164
15.8 %
93
9.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
18
315
1.4%
23
7.3%
277
87.9%
18
5.7 %
14
4.4 %
1
0.3 %
Parallel - Herd or Group Slaughter
10
358
1.6%
24
6.7%
326
91.1%
28
7.8 %
8
2.2 %
0
Parallel New Infection
9
212
1.0%
13
6.1%
190
89.6%
14
6.6 %
8
3.8 %
1
0.5 %
Parallel - Other
3
15
0.1%
5
33.3%
10
66.7%
2
13.3 %
0
0
Parallel Persistent Infection
30
1602
7.3%
88
5.5%
1455
90.8%
81
5.1 %
59
3.7 %
0
Parallel - Rapid Testing Two-times IRs
24
69
0.3%
14
20.3%
52
75.4%
4
5.8 %
2
2.9 %
1
1.4 %
Wales - High TB
94
2571
11.7%
167
6.5%
2310
89.8%
147
5.7 %
91
3.5 %
3
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
99
0.5%
5
5.1%
90
90.9%
6
6.1 %
4
4.0 %
0
Parallel New Infection
5
247
1.1%
22
8.9%
220
89.1%
17
6.9 %
5
2.0 %
0
Parallel - Other
3
5
0.0%
0
0.0 %
4
80.0%
1
20.0 %
1
20.0 %
0
Parallel Persistent Infection
1
234
1.1%
11
4.7%
217
92.7%
16
6.8 %
6
2.6 %
0
Parallel - Rapid Testing Two-times IRs
5
8
0.0%
3
37.5%
5
62.5%
0
0
0
Wales - Intermediate TB
19
593
2.7%
41
6.9%
536
90.4%
40
6.7 %
16
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel New Infection
1
52
0.2%
2
3.8%
49
94.2%
4
7.7 %
1
1.9 %
0
Parallel - Other
2
18
0.1%
1
5.6%
15
83.3%
3
16.7 %
2
11.1 %
0
Wales - Low TB
4
72
0.3%
3
4.2%
65
90.3%
8
11.1 %
4
5.6 %
0
Scotland - Scotland
Parallel New Infection
1
16
0.1%
0
0.0 %
14
87.5%
5
31.3 %
2
12.5 %
0
Parallel Persistent Infection
1
338
1.5%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Scotland - Scotland
2
354
1.6%
2
0.6%
343
96.9%
12
3.4 %
9
2.5 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
156
5402
2.4%
367
6.8%
4733
87.6%
276
5.1 %
201
3.7 %
101
1.9 %
Parallel - Herd or Group Slaughter
119
4435
2.0%
352
7.9%
3806
85.8%
345
7.8 %
270
6.1 %
8
0.2 %
Parallel New Infection
899 103483
46.1%
3628
3.5%
93027
89.9%
7677
7.4 %
6054
5.9 %
792
0.8 %
Parallel - new infection cull area
413
47094
21.0%
2383
5.1%
41822
88.8%
3240
6.9 %
2525
5.4 %
364
0.8 %
Parallel - Other
69
3959
1.8%
257
6.5%
3283
82.9%
457
11.5 %
377
9.5 %
42
1.1 %
Parallel Persistent Infection
389
45324
20.2%
1946
4.3%
40523
89.4%
2877
6.3 %
2216
4.9 %
642
1.4 %
Parallel - persistent infection cull area
68
13972
6.2%
778
5.6%
12399
88.7%
1007
7.2 %
788
5.6 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
305
886
0.4%
183
20.7%
633
71.4%
61
6.9 %
43
4.9 %
27
3.0 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
9894
4.4%
200251
89.2%
15941
7.1 %
12475
5.6 %
1983
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA
Extended Flexible IFN-GAMMA test
22
1606
0.7%
79
4.9%
1422
88.5%
95
5.9 %
60
3.7 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
19
xx-xxxx-xxxx
0.7%
136
8.3%
1325
80.6%
228
13.9 %
182
11.1 %
0
Parallel - new infection cull area
412
46834
20.9%
2364
5.0%
41593
88.8%
3228
6.9 %
2513
5.4 %
364
0.8 %
Parallel - Other
20
1542
0.7%
83
5.4%
1172
76.0%
284
18.4 %
245
15.9 %
42
2.7 %
Parallel Persistent Infection
118
19743
8.8%
950
4.8%
17268
87.5%
1320
6.7 %
998
5.1 %
528
2.7 %
Parallel - persistent infection cull area
68
13972
6.2%
778
5.6%
12399
88.7%
1007
7.2 %
788
5.6 %
7
0.1 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
674
84045
37.4%
2881
3.4%
75695
90.1%
6113
7.3 %
4802
5.7 %
685
0.8 %
Parallel - new infection cull area
1
260
0.1%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
14
1314
0.6%
27
2.1%
1234
93.9%
66
5.0 %
53
4.0 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
66
8165
3.6%
257
3.1%
7252
88.8%
758
9.3 %
618
7.6 %
38
0.5 %
Parallel Persistent Infection
5
736
0.3%
3
0.4%
561
76.2%
89
12.1 %
79
10.7 %
93
12.6 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
England
xx-xxxx-xxxx
80.1%
7586
4.2%
160268
89.0%
13209
7.3 %
10356
5.8 %
1802
1.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
93
2726
1.2%
194
7.1%
2434
89.3%
123
4.5 %
95
3.5 %
3
0.1 %
Parallel - Herd or Group Slaughter
107
4277
1.9%
327
7.6%
3689
86.3%
327
7.6 %
254
5.9 %
8
0.2 %
Parallel New Infection
29
1009
0.4%
56
5.6%
905
89.7%
55
5.5 %
47
4.7 %
1
0.1 %
Parallel - Other
33
1840
0.8%
122
6.6%
1619
88.0%
133
7.2 %
99
5.4 %
0
Parallel Persistent Infection
235
21183
9.4%
883
4.2%
19268
91.0%
1293
6.1 %
1013
4.8 %
21
0.1 %
Parallel - Rapid Testing Two-times IRs
265
773
0.3%
164
21.2%
542
70.1%
58
7.5 %
40
5.2 %
27
3.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
37
931
0.4%
89
9.6%
747
80.2%
51
5.5 %
42
4.5 %
53
5.7 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
75
6018
2.7%
199
3.3%
5460
90.7%
395
6.6 %
307
5.1 %
52
0.9 %
Parallel - Other
14
559
0.2%
51
9.1%
477
85.3%
37
6.6 %
31
5.5 %
0
Parallel Persistent Infection
14
1581
0.7%
74
4.7%
1454
92.0%
85
5.4 %
53
3.4 %
0
Parallel - Rapid Testing Two-times IRs
38
111
0.0%
19
17.1%
89
80.2%
3
2.7 %
3
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
22
731
0.3%
18
2.5%
674
92.2%
35
4.8 %
23
3.1 %
16
2.2 %
Parallel - Other
2
18
0.0%
1
5.6%
15
83.3%
3
16.7 %
2
11.1 %
0
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales
976
42378
18.9%
2225
5.3%
37938
89.5%
2632
6.2 %
2037
4.8 %
181
0.4 %
Scotland - Scotland
Parallel New Infection
14
1872
0.8%
81
4.3%
1716
91.7%
93
5.0 %
75
4.0 %
0
Parallel Persistent Infection
1
338
0.2%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Scotland
15
2210
1.0%
83
3.8%
2045
92.5%
100
4.5 %
82
3.7 %
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 September
216
21441
137
946
4
6
0
0
0
0
33
1
0
2
0
842
0
51
1
4
October
274
23976
152
1417
1
8
0
0
0
0
100
2
0
3
0
1242
0
44
0
2
November
282
24570
192
1580
2
4
0
0
0
0
42
5
0
8
2
1336
0
169
0
10
December
228
21554
147
1241
6
8
0
0
0
0
92
2
0
7
1
1037
0
82
0
5
2019 January
289
27513
179
1283
5
3
0
0
0
0
86
3
0
3
0
1117
0
63
0
0
February
320
28044
216
1907
2
8
0
0
0
0
166
5
0
9
0
1634
0
74
2
4
March
315
29624
199
1546
1
0
0
0
0
135
0
0
4
0
1316
0
87
0
3
April
269
30320
162
1135
1
8
0
0
0
0
87
3
0
5
0
989
0
40
0
0
May
364
28357
176
1116
2
0
0
0
0
99
0
0
5
0
962
6
42
0
0
June
287
26287
159
964
5
13
0
0
0
0
94
0
0
4
1
773
19
54
0
0
July
316
32549
201
1219
1
32
0
0
0
0
109
0
11
3
0
1001
60
0
0
0
August
262
21887
126
724
23
157
0
0
0
0
59
0
3
0
0
372
46
0
0
0
Totals (last
12 months)
3422
316122
2046
15078
50
250
0
0
0
0
1102
21
14
53
4
12621
131
706
3
28
Table 5a. Summary of PM and culture results for IFNg positive animals.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1994
249158
1311
11207
179
0
0
0
0
928
13
11
35
3
9845
13
94
0
2
Scotland
17
2225
10
83
0
0
0
0
0
12
0
0
2
0
61
1
4
0
0
Wales
1411
64739
725
3788
71
0
0
0
0
162
8
3
16
1
2715
117
608
3
26
Totals
3422
316122
2046
15078
250
0
0
0
0
1102
21
14
53
4
12621
131
706
3
28
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2017
2018
2019
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2017
2018
2019
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
2017
2018
2019
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
W Sussex
W Glamorgan
Staffs
Shropshire
Pembs
N Yorks
Northants
Leics & Rut
Heref
Gwent
Gloucs
Dumfries & G
Devon
Cumbria
Cheshire
Carms
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during August 2019 (% submission per
County)
County
%Resampled %Retested
Avon
6.67 %
6.67 %
Berks
10.00 %
10.77 %
Bucks
20.43 %
22.58 %
Carms
3.11 %
4.67 %
Ceredigion
3.98 %
6.42 %
Cheshire
5.07 %
7.93 %
Corn & Scilly
6.47 %
8.47 %
Cumbria
33.33 %
44.44 %
Derbyshire
6.73 %
9.57 %
Devon
7.71 %
10.26 %
Dorset
4.33 %
7.09 %
Dumfries & G
12.50 %
31.25 %
Gloucestershire
5.36 %
6.25 %
Gloucs
37.39 %
39.13 %
Gr London
4.65 %
9.30 %
Gwent
22.22 %
22.22 %
Hampshire
9.93 %
13.79 %
Heref
3.00 %
3.67 %
Kirkcudbright
2.07 %
2.07 %
Leics & Rut
5.88 %
17.65 %
NE Wales
3.05 %
7.32 %
Northants
Northumb
28.09 %
30.41 %
N Yorks
3.98 %
5.61 %
Oxon
9.34 %
11.98 %
Pembs
3.55 %
6.09 %
Powys
3.45 %
6.03 %
Shropshire
17.81 %
20.55 %
Somerset excl
3.92 %
5.81 %
Staffs
9.38 %
10.94 %
Warks
4.92 %
6.03 %
W Glamorgan
Wiltshire
7.90 %
10.14 %
W Sussex
60.87 %
65.22 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
17871
511
2.9%
18297
511
2.8%
Wales
3203
211
6.6%
3236
211
6.5%
Scotland
354
2
0.6%
354
2
0.6%
GB
21428
724
3.4%
21887
724
3.3%
Table 6a (i): Number of animals and submissions tested in August 2019
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
165650
7586
4.6%
179993
7586
4.2%
Wales
38635
2225
5.8%
42378
2225
5.3%
Scotland
1889
83
4.4%
2210
83
3.8%
GB
206170
9894
4.8%
224581
9894
4.4%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of August
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
15428
592
3.8%
15697
592
3.8%
Wales
3771
87
2.3%
3849
87
2.3%
Scotland
49
3
6.1%
53
3
5.7%
GB
19248
682
3.5%
19599
682
3.5%
Table 6a (ii): Number of animals and submissions tested in August 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of August
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
110791
5999
5.4%
124473
5999
4.8%
Wales
31928
1306
4.1%
35246
1306
3.7%
Scotland
2066
181
8.8%
2226
181
8.1%
GB
144772
7486
5.2%
161945
7486
4.6%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
1
0
0
Total
1
1
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples August 2019
Table 7b Number of private samples 2019
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
1
6
0
0
PRV-SP
1
1
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Somerset excl North
PRV-SE
4
41
15
36.59 %
PRV-SP
3
3
0
0
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
13
65
15
23.08 %
Isle of Man
SER-IOM
20
116
1
0.86 %
Isle of Man
SER-IOM
7
64
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
4
146
12
8.2%
Ceredigion
2
343
10
2.9%
North East Wales
7
270
37
13.7%
Pembrokeshire
2
70
6
8.6%
Powys
2
45
4
8.9%
South Wales
1
9
0
0
Wiltshire
1
248
9
3.6%
TC0077
Sum:
1,131
78
6.9 %
Table 8a Number of Cattle Antibody Tests August 2019
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
12
427
34
8.0%
Ceredigion
3
372
17
4.6%
North East Wales
19
737
103
14.0%
Pembrokeshire
14
465
27
5.8%
Powys
6
313
18
5.8%
South Wales
5
196
16
8.2%
Wiltshire
1
248
9
3.6%
TC0077
Sum:
2,758
224
8.1 %
Table 8b Number of Cattle Antibody Tests 2019