Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - January 2022
Source: environment.data.gov.uk (2023-01-25)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2022. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 185
Report Period
Issued 10 February 2022

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
January
2022
%
Total 2022
%
Submissions
262
262
Samples
Total (%)
18563
100.00 %
18563
100.00 %
England
15074
81.20 %
15074
81.20 %
Wales
3487
18.78 %
3487
18.78 %
Scotland
2
0.01 %
2
0.01 %
Parallel Tests
PAR-CUL-N
2389
12.87 %
2389
12.87 %
PAR-CUL-P
1742
9.38 %
1742
9.38 %
PAR-HERD-S
34
0.18 %
34
0.18 %
PAR-IR-EX
23
0.12 %
23
0.12 %
PAR-IR-SEV
128
0.69 %
128
0.69 %
PAR-NEW-IN
2768
14.91 %
2768
14.91 %
PAR-OTHER
591
3.18 %
591
3.18 %
PAR-PERSIS
3508
18.90 %
3508
18.90 %
PAR-POSTM
57
0.31 %
57
0.31 %
PAR-RAPID
98
0.53 %
98
0.53 %
PAR-RECUR
5824
31.37 %
5824
31.37 %
Total(% of all samples)
17162
92.45 %
17162
92.45 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
1398
7.53 %
1398
7.53 %
SER-FRAUD
0
0.00 %
SER-NSR
1
0.01 %
1
0.01 %
SER-OTHER
2
0.01 %
2
0.01 %
Total(% of all samples)
1401
7.55 %
1401
7.55 %
Total
18563
100.00 %
18563
100.00 %
Retests
Total (% of all samples)
1395
7.51 %
1395
7.51 %
England
1124
6.06 %
1124
6.06 %
Wales
271
1.46 %
271
1.46 %
Scotland
Resamples
Total (% of all samples)
1239
6.67 %
1239
6.67 %
England
1003
5.40 %
1003
5.40 %
Wales
236
1.27 %
236
1.27 %
Scotland
Rejects
Total (% of all samples)
405
2.18 %
405
2.18 %
England
330
1.78 %
330
1.78 %
Wales
75
0.40 %
75
0.40 %
Scotland
0
0.00 %
Table 1. Monthly Totals for January 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-NSR
SER-OTHER
Breakdown of
Reason for
submission
PAR-CUL-N
2389
12.9 %
PAR-CUL-P
1742
9.4 %
PAR-HERD-S
34
0.2 %
PAR-IR-EX
23
0.1 %
PAR-IR-SEV
128
0.7 %
PAR-NEW-IN
2768
14.9 %
PAR-OTHER
591
3.2 %
PAR-PERSIS
3508
18.9 %
PAR-POSTM
57
0.3 %
PAR-RAPID
98
0.5 %
PAR-RECUR
5824
31.4 %
SER-FLEXI
1398
7.5 %
SER-NSR
1
0.0 %
SER-OTHER
2
0.0 %
Total
18563
100.00 %
Figure 1. Number of samples submitted in each category during January 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
Argyll & Bute
W Glamorgan
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
Northumb
Northants
Leics & Rut
Lancashire
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during January 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
500
1000
1500
2000
2500
3000
3500
Argyll & Bute
W Glamorgan
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Wiltshire
Staffs
Somerset excl N
Shropshire
Oxon
Northumb
Northants
Leics & Rut
Lancashire
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
2
513
2.76 %
25
4.87 %
474
92.40 %
14
2.73 %
13
2.53 %
1
0.19 %
PAR-RECUR
3
271
1.46 %
4
1.48 %
246
90.77 %
23
8.49 %
19
7.01 %
2
0.74 %
Corn & Scilly
PAR-CUL-N
1
2
0.01 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
1,656
8.92 %
72
4.35 %
1476
89.13 %
126
7.61 %
108
6.52 %
0
0.00 %
PAR-RECUR
4
49
0.26 %
1
2.04 %
29
59.18 %
7
14.29 %
19
38.78 %
0
0.00 %
Devon
PAR-CUL-N
3
642
3.46 %
33
5.14 %
557
86.76 %
56
8.72 %
51
7.94 %
1
0.16 %
PAR-CUL-P
2
411
2.21 %
8
1.95 %
382
92.94 %
28
6.81 %
19
4.62 %
2
0.49 %
PAR-PERSIS
3
165
0.89 %
5
3.03 %
140
84.85 %
20
12.12 %
20
12.12 %
0
0.00 %
PAR-RECUR
14
1,859
10.01 %
79
4.25 %
xx-xxxx-xxxx
88.22 %
160
8.61 %
139
7.48 %
1
0.05 %
Gloucs
PAR-CUL-N
4
43
0.23 %
4
9.30 %
28
65.12 %
11
25.58 %
11
25.58 %
0
0.00 %
PAR-CUL-P
1
195
1.05 %
7
3.59 %
170
87.18 %
17
8.72 %
17
8.72 %
1
0.51 %
PAR-RECUR
1
18
0.10 %
2
11.11 %
10
55.56 %
6
33.33 %
6
33.33 %
0
0.00 %
Heref
PAR-PERSIS
1
359
1.93 %
22
6.13 %
316
88.02 %
27
7.52 %
21
5.85 %
0
0.00 %
PAR-RECUR
5
346
1.86 %
13
3.76 %
316
91.33 %
23
6.65 %
17
4.91 %
0
0.00 %
Shropshire
PAR-OTHER
2
212
1.14 %
4
1.89 %
202
95.28 %
7
3.30 %
6
2.83 %
0
0.00 %
PAR-RECUR
10
1,432
7.71 %
32
2.23 %
1287
89.87 %
119
8.31 %
113
7.89 %
0
0.00 %
Somerset excl N
PAR-PERSIS
1
217
1.17 %
3
1.38 %
200
92.17 %
17
7.83 %
14
6.45 %
0
0.00 %
PAR-RECUR
3
241
1.30 %
10
4.15 %
224
92.95 %
18
7.47 %
7
2.90 %
0
0.00 %
Staffs
PAR-CUL-N
1
7
0.04 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-CUL-P
1
425
2.29 %
11
2.59 %
401
94.35 %
17
4.00 %
13
3.06 %
0
0.00 %
PAR-PERSIS
1
521
2.81 %
12
2.30 %
253
48.56 %
35
6.72 %
35
6.72 %
221
42.42 %
PAR-RECUR
4
624
3.36 %
34
5.45 %
501
80.29 %
26
4.17 %
18
2.88 %
71
11.38 %
Wiltshire
PAR-CUL-N
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-CUL-P
2
711
3.83 %
53
7.45 %
595
83.68 %
52
7.31 %
48
6.75 %
15
2.11 %
PAR-NEW-IN
1
19
0.10 %
1
5.26 %
13
68.42 %
5
26.32 %
5
26.32 %
0
0.00 %
PAR-PERSIS
2
70
0.38 %
7
10.00 %
46
65.71 %
20
28.57 %
17
24.29 %
0
0.00 %
Table 2a. Summary by County for January 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
404
2.18 %
32
7.92 %
343
84.90 %
30
7.43 %
27
6.68 %
2
0.50 %
England - Edge
Bucks
PAR-NEW-IN
1
13
0.07 %
0
0.00 %
12
92.31 %
1
7.69 %
1
7.69 %
0
0.00 %
Cheshire
PAR-CUL-N
2
1,180
6.36 %
25
2.12 %
1102
93.39 %
65
5.51 %
53
4.49 %
0
0.00 %
PAR-NEW-IN
2
397
2.14 %
15
3.78 %
357
89.92 %
28
7.05 %
25
6.30 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
180
0.97 %
3
1.67 %
162
90.00 %
16
8.89 %
15
8.33 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
6
0.03 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
431
2.32 %
18
4.18 %
397
92.11 %
17
3.94 %
16
3.71 %
0
0.00 %
Northants
PAR-NEW-IN
1
282
1.52 %
9
3.19 %
232
82.27 %
44
15.60 %
41
14.54 %
0
0.00 %
PAR-RECUR
1
114
0.61 %
1
0.88 %
93
81.58 %
11
9.65 %
20
17.54 %
0
0.00 %
Oxon
PAR-NEW-IN
4
491
2.65 %
35
7.13 %
423
86.15 %
38
7.74 %
33
6.72 %
0
0.00 %
PAR-PERSIS
2
160
0.86 %
15
9.38 %
136
85.00 %
9
5.63 %
9
5.63 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
2
33
0.18 %
0
0.00 %
31
93.94 %
2
6.06 %
2
6.06 %
0
0.00 %
Lancashire
SER-NSR
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northumb
PAR-NEW-IN
1
248
1.34 %
10
4.03 %
208
83.87 %
19
7.66 %
17
6.85 %
13
5.24 %
W Sussex
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
1
121
0.65 %
3
2.48 %
111
91.74 %
8
6.61 %
7
5.79 %
0
0.00 %
England
108
15074
81.20 %
609
4.04 %
13132
87.12 %
1124
7.46 %
1003
6.65 %
330
2.19 %
Wales - High TB
Carms
PAR-IR-SEV
4
28
0.15 %
0
0.00 %
13
46.43 %
2
7.14 %
2
7.14 %
13
46.43 %
PAR-RAPID
4
15
0.08 %
1
6.67 %
8
53.33 %
0
0.00 %
0
0.00 %
6
40.00 %
PAR-RECUR
2
22
0.12 %
5
22.73 %
14
63.64 %
5
22.73 %
3
13.64 %
0
0.00 %
Ceredigion
PAR-IR-EX
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
4
0.02 %
0
0.00 %
2
50.00 %
0
0.00 %
0
0.00 %
2
50.00 %
PAR-RAPID
2
4
0.02 %
1
25.00 %
0
0.00 %
1
25.00 %
1
25.00 %
2
50.00 %
PAR-RECUR
1
31
0.17 %
10
32.26 %
21
67.74 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
51
0.27 %
2
3.92 %
47
92.16 %
3
5.88 %
2
3.92 %
0
0.00 %
Gwent
PAR-IR-SEV
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
3
70
0.38 %
0
0.00 %
66
94.29 %
6
8.57 %
3
4.29 %
1
1.43 %
Pembs
PAR-IR-EX
3
15
0.08 %
3
20.00 %
12
80.00 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
14
66
0.36 %
8
12.12 %
50
75.76 %
4
6.06 %
3
4.55 %
5
7.58 %
PAR-PERSIS
2
133
0.72 %
4
3.01 %
118
88.72 %
11
8.27 %
11
8.27 %
0
0.00 %
PAR-RAPID
13
41
0.22 %
8
19.51 %
30
73.17 %
2
4.88 %
2
4.88 %
1
2.44 %
PAR-RECUR
5
228
1.23 %
11
4.82 %
210
92.11 %
8
3.51 %
5
2.19 %
2
0.88 %
SER-FLEXI
9
566
3.05 %
78
13.78 %
415
73.32 %
47
8.30 %
43
7.60 %
31
5.48 %
Powys
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
11
0.06 %
0
0.00 %
9
81.82 %
3
27.27 %
2
18.18 %
0
0.00 %
PAR-OTHER
5
163
0.88 %
9
5.52 %
137
84.05 %
18
11.04 %
17
10.43 %
0
0.00 %
PAR-PERSIS
1
36
0.19 %
1
2.78 %
31
86.11 %
5
13.89 %
4
11.11 %
0
0.00 %
PAR-RAPID
3
7
0.04 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
8
0.04 %
0
0.00 %
7
87.50 %
2
25.00 %
1
12.50 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
4
100.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
2
100.00 %
PAR-RAPID
1
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
13
0.07 %
2
15.38 %
11
84.62 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
148
0.80 %
2
1.35 %
142
95.95 %
4
2.70 %
4
2.70 %
0
0.00 %
PAR-PERSIS
3
191
1.03 %
3
1.57 %
169
88.48 %
18
9.42 %
18
9.42 %
1
0.52 %
PAR-POSTM
5
11
0.06 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
397
2.14 %
33
8.31 %
347
87.41 %
17
4.28 %
16
4.03 %
1
0.25 %
Powys
PAR-NEW-IN
1
204
1.10 %
2
0.98 %
192
94.12 %
8
3.92 %
8
3.92 %
2
0.98 %
PAR-OTHER
2
214
1.15 %
8
3.74 %
182
85.05 %
28
13.08 %
24
11.21 %
0
0.00 %
PAR-RAPID
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
6
0.03 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
1
107
0.58 %
2
1.87 %
99
92.52 %
6
5.61 %
6
5.61 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for January 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Low TB
Gwynedd
PAR-NEW-IN
4
55
0.30 %
1
1.82 %
45
81.82 %
10
18.18 %
9
16.36 %
0
0.00 %
PAR-POSTM
7
21
0.11 %
0
0.00 %
20
95.24 %
1
4.76 %
1
4.76 %
0
0.00 %
NE Wales
PAR-HERD-S
1
34
0.18 %
2
5.88 %
26
76.47 %
6
17.65 %
6
17.65 %
0
0.00 %
PAR-NEW-IN
2
137
0.74 %
0
0.00 %
125
91.24 %
14
10.22 %
10
7.30 %
2
1.46 %
PAR-POSTM
12
25
0.13 %
1
4.00 %
24
96.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
8
0.04 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
SER-FLEXI
2
384
2.07 %
38
9.90 %
312
81.25 %
40
10.42 %
34
8.85 %
0
0.00 %
Wales
153
3487
18.78 %
243
6.97 %
2934
84.14 %
271
7.77 %
236
6.77 %
75
2.15 %
Scotland - Scotland
Argyll & Bute
SER-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Scotland
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Grand Total
Sum:
262
18563
852
16068
1395
1239
405
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
2
513
2.76 %
25
4.87 %
474
92.40 %
14
2.73 %
13
2.53 %
1
0.19 %
PAR-RECUR
3
271
1.46 %
4
1.48 %
246
90.77 %
23
8.49 %
19
7.01 %
2
0.74 %
Corn & Scilly
PAR-CUL-N
1
2
0.01 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
1,656
8.92 %
72
4.35 %
1476
89.13 %
126
7.61 %
108
6.52 %
0
0.00 %
PAR-RECUR
4
49
0.26 %
1
2.04 %
29
59.18 %
7
14.29 %
19
38.78 %
0
0.00 %
Devon
PAR-CUL-N
3
642
3.46 %
33
5.14 %
557
86.76 %
56
8.72 %
51
7.94 %
1
0.16 %
PAR-CUL-P
2
411
2.21 %
8
1.95 %
382
92.94 %
28
6.81 %
19
4.62 %
2
0.49 %
PAR-PERSIS
3
165
0.89 %
5
3.03 %
140
84.85 %
20
12.12 %
20
12.12 %
0
0.00 %
PAR-RECUR
14
1,859
10.01 %
79
4.25 %
xx-xxxx-xxxx
88.22 %
160
8.61 %
139
7.48 %
1
0.05 %
Gloucs
PAR-CUL-N
4
43
0.23 %
4
9.30 %
28
65.12 %
11
25.58 %
11
25.58 %
0
0.00 %
PAR-CUL-P
1
195
1.05 %
7
3.59 %
170
87.18 %
17
8.72 %
17
8.72 %
1
0.51 %
PAR-RECUR
1
18
0.10 %
2
11.11 %
10
55.56 %
6
33.33 %
6
33.33 %
0
0.00 %
Heref
PAR-PERSIS
1
359
1.93 %
22
6.13 %
316
88.02 %
27
7.52 %
21
5.85 %
0
0.00 %
PAR-RECUR
5
346
1.86 %
13
3.76 %
316
91.33 %
23
6.65 %
17
4.91 %
0
0.00 %
Shropshire
PAR-OTHER
2
212
1.14 %
4
1.89 %
202
95.28 %
7
3.30 %
6
2.83 %
0
0.00 %
PAR-RECUR
10
1,432
7.71 %
32
2.23 %
1287
89.87 %
119
8.31 %
113
7.89 %
0
0.00 %
Somerset excl N
PAR-PERSIS
1
217
1.17 %
3
1.38 %
200
92.17 %
17
7.83 %
14
6.45 %
0
0.00 %
PAR-RECUR
3
241
1.30 %
10
4.15 %
224
92.95 %
18
7.47 %
7
2.90 %
0
0.00 %
Staffs
PAR-CUL-N
1
7
0.04 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-CUL-P
1
425
2.29 %
11
2.59 %
401
94.35 %
17
4.00 %
13
3.06 %
0
0.00 %
PAR-PERSIS
1
521
2.81 %
12
2.30 %
253
48.56 %
35
6.72 %
35
6.72 %
221
42.42 %
PAR-RECUR
4
624
3.36 %
34
5.45 %
501
80.29 %
26
4.17 %
18
2.88 %
71
11.38 %
Wiltshire
PAR-CUL-N
2
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-CUL-P
2
711
3.83 %
53
7.45 %
595
83.68 %
52
7.31 %
48
6.75 %
15
2.11 %
PAR-NEW-IN
1
19
0.10 %
1
5.26 %
13
68.42 %
5
26.32 %
5
26.32 %
0
0.00 %
PAR-PERSIS
2
70
0.38 %
7
10.00 %
46
65.71 %
20
28.57 %
17
24.29 %
0
0.00 %
Table 2b. Summary by County for 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
2
404
2.18 %
32
7.92 %
343
84.90 %
30
7.43 %
27
6.68 %
2
0.50 %
England - Edge
Bucks
PAR-NEW-IN
1
13
0.07 %
0
0.00 %
12
92.31 %
1
7.69 %
1
7.69 %
0
0.00 %
Cheshire
PAR-CUL-N
2
1,180
6.36 %
25
2.12 %
1102
93.39 %
65
5.51 %
53
4.49 %
0
0.00 %
PAR-NEW-IN
2
397
2.14 %
15
3.78 %
357
89.92 %
28
7.05 %
25
6.30 %
0
0.00 %
Derbyshire
PAR-NEW-IN
3
180
0.97 %
3
1.67 %
162
90.00 %
16
8.89 %
15
8.33 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
6
0.03 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
431
2.32 %
18
4.18 %
397
92.11 %
17
3.94 %
16
3.71 %
0
0.00 %
Northants
PAR-NEW-IN
1
282
1.52 %
9
3.19 %
232
82.27 %
44
15.60 %
41
14.54 %
0
0.00 %
PAR-RECUR
1
114
0.61 %
1
0.88 %
93
81.58 %
11
9.65 %
20
17.54 %
0
0.00 %
Oxon
PAR-NEW-IN
4
491
2.65 %
35
7.13 %
423
86.15 %
38
7.74 %
33
6.72 %
0
0.00 %
PAR-PERSIS
2
160
0.86 %
15
9.38 %
136
85.00 %
9
5.63 %
9
5.63 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
2
33
0.18 %
0
0.00 %
31
93.94 %
2
6.06 %
2
6.06 %
0
0.00 %
Lancashire
SER-NSR
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northumb
PAR-NEW-IN
1
248
1.34 %
10
4.03 %
208
83.87 %
19
7.66 %
17
6.85 %
13
5.24 %
W Sussex
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
1
121
0.65 %
3
2.48 %
111
91.74 %
8
6.61 %
7
5.79 %
0
0.00 %
England
108
15074
81.20 %
609
4.04 %
13132
87.12 %
1124
7.46 %
1003
6.65 %
330
2.19 %
Wales - High TB
Carms
PAR-IR-SEV
4
28
0.15 %
0
0.00 %
13
46.43 %
2
7.14 %
2
7.14 %
13
46.43 %
PAR-RAPID
4
15
0.08 %
1
6.67 %
8
53.33 %
0
0.00 %
0
0.00 %
6
40.00 %
PAR-RECUR
2
22
0.12 %
5
22.73 %
14
63.64 %
5
22.73 %
3
13.64 %
0
0.00 %
Ceredigion
PAR-IR-EX
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
4
0.02 %
0
0.00 %
2
50.00 %
0
0.00 %
0
0.00 %
2
50.00 %
PAR-RAPID
2
4
0.02 %
1
25.00 %
0
0.00 %
1
25.00 %
1
25.00 %
2
50.00 %
PAR-RECUR
1
31
0.17 %
10
32.26 %
21
67.74 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
51
0.27 %
2
3.92 %
47
92.16 %
3
5.88 %
2
3.92 %
0
0.00 %
Gwent
PAR-IR-SEV
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
3
70
0.38 %
0
0.00 %
66
94.29 %
6
8.57 %
3
4.29 %
1
1.43 %
Pembs
PAR-IR-EX
3
15
0.08 %
3
20.00 %
12
80.00 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
14
66
0.36 %
8
12.12 %
50
75.76 %
4
6.06 %
3
4.55 %
5
7.58 %
PAR-PERSIS
2
133
0.72 %
4
3.01 %
118
88.72 %
11
8.27 %
11
8.27 %
0
0.00 %
PAR-RAPID
13
41
0.22 %
8
19.51 %
30
73.17 %
2
4.88 %
2
4.88 %
1
2.44 %
PAR-RECUR
5
228
1.23 %
11
4.82 %
210
92.11 %
8
3.51 %
5
2.19 %
2
0.88 %
SER-FLEXI
9
566
3.05 %
78
13.78 %
415
73.32 %
47
8.30 %
43
7.60 %
31
5.48 %
Powys
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
11
0.06 %
0
0.00 %
9
81.82 %
3
27.27 %
2
18.18 %
0
0.00 %
PAR-OTHER
5
163
0.88 %
9
5.52 %
137
84.05 %
18
11.04 %
17
10.43 %
0
0.00 %
PAR-PERSIS
1
36
0.19 %
1
2.78 %
31
86.11 %
5
13.89 %
4
11.11 %
0
0.00 %
PAR-RAPID
3
7
0.04 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
8
0.04 %
0
0.00 %
7
87.50 %
2
25.00 %
1
12.50 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
4
100.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
2
100.00 %
PAR-RAPID
1
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
2
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
13
0.07 %
2
15.38 %
11
84.62 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
148
0.80 %
2
1.35 %
142
95.95 %
4
2.70 %
4
2.70 %
0
0.00 %
PAR-PERSIS
3
191
1.03 %
3
1.57 %
169
88.48 %
18
9.42 %
18
9.42 %
1
0.52 %
PAR-POSTM
5
11
0.06 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
397
2.14 %
33
8.31 %
347
87.41 %
17
4.28 %
16
4.03 %
1
0.25 %
Powys
PAR-NEW-IN
1
204
1.10 %
2
0.98 %
192
94.12 %
8
3.92 %
8
3.92 %
2
0.98 %
PAR-OTHER
2
214
1.15 %
8
3.74 %
182
85.05 %
28
13.08 %
24
11.21 %
0
0.00 %
PAR-RAPID
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
6
0.03 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
1
107
0.58 %
2
1.87 %
99
92.52 %
6
5.61 %
6
5.61 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Low TB
Gwynedd
PAR-NEW-IN
4
55
0.30 %
1
1.82 %
45
81.82 %
10
18.18 %
9
16.36 %
0
0.00 %
PAR-POSTM
7
21
0.11 %
0
0.00 %
20
95.24 %
1
4.76 %
1
4.76 %
0
0.00 %
NE Wales
PAR-HERD-S
1
34
0.18 %
2
5.88 %
26
76.47 %
6
17.65 %
6
17.65 %
0
0.00 %
PAR-NEW-IN
2
137
0.74 %
0
0.00 %
125
91.24 %
14
10.22 %
10
7.30 %
2
1.46 %
PAR-POSTM
12
25
0.13 %
1
4.00 %
24
96.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
8
0.04 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
SER-FLEXI
2
384
2.07 %
38
9.90 %
312
81.25 %
40
10.42 %
34
8.85 %
0
0.00 %
Wales
153
3487
18.78 %
243
6.97 %
2934
84.14 %
271
7.77 %
236
6.77 %
75
2.15 %
Scotland - Scotland
Argyll & Bute
SER-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Scotland
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Grand Total
Sum:
262
18563
852
16068
1395
1239
405
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol January 2022
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
107
15073
100.0%
609
4.0%
13131
87.1%
1124
7.5%
1003
6.7%
330
2.2%
Serial/Extended
1
1
0.0%
0
0.0 %
1 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
108
15074
100.0%
609
4.0%
13132
87.1%
1124
7.5%
1003
6.7%
330
2.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
1
2
100.0%
0
0.0 %
2 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
2
100.0%
0
0.0 %
2 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
133
2089
59.9%
92
4.4%
1813
86.8%
164
7.9%
141
6.7%
43
2.1%
Serial/Extended
20
1398
40.1%
151
10.8%
1121
80.2%
107
7.7%
95
6.8%
32
2.3%
Total
153
3487
100.0%
243
7.0%
2934
84.1%
271
7.8%
236
6.8%
75
2.2%
GB
Parallel
240
17162
92.5%
701
4.1%
14944
87.1%
1288
7.5%
1144
6.7%
373
2.2%
Serial/Extended
22
1401
7.5%
151
10.8%
1124
80.2%
107
7.6%
95
6.8%
32
2.3%
Total
262
18563
100.0%
852
4.6%
16068
86.6%
1395
7.5%
1239
6.7%
405
2.2%
108.00
153.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2022)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
107
15073
100.0%
609
4.0%
13131
87.1%
1124
7.5%
1003
6.7%
330
2.2%
Serial/Extended
1
1
0.0%
0
0.0 %
1 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
108
15074
100.0%
609
4.0%
13132
87.1%
1124
7.5%
1003
6.7%
330
2.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
1
2
100.0%
0
0.0 %
2 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
2
100.0%
0
0.0 %
2 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
133
2089
59.9%
92
4.4%
1813
86.8%
164
7.9%
141
6.7%
43
2.1%
Serial/Extended
20
1398
40.1%
151
10.8%
1121
80.2%
107
7.7%
95
6.8%
32
2.3%
Total
153
3487
100.0%
243
7.0%
2934
84.1%
271
7.8%
236
6.8%
75
2.2%
GB
Parallel
240
17162
92.5%
701
4.1%
14944
87.1%
1288
7.5%
1144
6.7%
373
2.2%
Serial/Extended
22
1401
7.5%
151
10.8%
1124
80.2%
107
7.6%
95
6.8%
32
2.3%
Total
262
18563
100.0%
852
4.6%
16068
86.6%
1395
7.5%
1239
6.7%
405
2.2%
108.00
153.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
1398
7.5%
151
10.8%
1121
80.2%
107
7.7 %
95
6.8 %
32
2.3 %
Parallel - Herd or Group Slaughter
1
34
0.2%
2
5.9%
26
76.5%
6
17.6 %
6
17.6 %
0
Parallel - IRs in Exit Strategy for persistent her
8
23
0.1%
4
17.4%
19
82.6%
1
4.3 %
0
0
Parallel New Infection
31
2768
14.9%
100
3.6%
2458
88.8%
214
7.7 %
193
7.0 %
17
0.6 %
Parallel - new infection cull area
15
2389
12.9%
87
3.6%
2171
90.9%
148
6.2 %
129
5.4 %
2
0.1 %
Parallel - Other
10
591
3.2%
21
3.6%
521
88.2%
53
9.0 %
47
8.0 %
2
0.3 %
Parallel Persistent Infection
22
3508
18.9%
144
4.1%
2885
82.2%
288
8.2 %
257
7.3 %
222
6.3 %
Parallel - persistent infection cull area
6
1742
9.4%
79
4.5%
1548
88.9%
114
6.5 %
97
5.6 %
18
1.0 %
Parallel – post-mvt test coupled with IDEXX an
24
57
0.3%
1
1.8%
55
96.5%
1
1.8 %
1
1.8 %
0
Parallel - Rapid Testing Two-times IRs
32
98
0.5%
17
17.3%
68
69.4%
4
4.1 %
4
4.1 %
9
9.2 %
Parallel - Recurrent breakdowns
61
5824
31.4%
236
4.1%
5106
87.7%
450
7.7 %
403
6.9 %
79
1.4 %
Parallel - severe IRs - in persistent herds
30
128
0.7%
10
7.8%
87
68.0%
9
7.0 %
7
5.5 %
24
18.8 %
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Total
262
18563
100.0%
852
4.6%
16068
86.6%
1395
7.5 %
1239
6.7 %
405
2.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (January 2022)
England - HRA
Parallel New Infection
1
19
0.1%
1
5.3%
13
68.4%
5
26.3 %
5
26.3 %
0
Parallel - new infection cull area
13
1209
6.5%
62
5.1%
1069
88.4%
83
6.9 %
76
6.3 %
2
0.2 %
Parallel - Other
2
212
1.1%
4
1.9%
202
95.3%
7
3.3 %
6
2.8 %
0
Parallel Persistent Infection
14
2988
16.1%
121
4.0%
2431
81.4%
245
8.2 %
215
7.2 %
221
7.4 %
Parallel - persistent infection cull area
6
1742
9.4%
79
4.5%
1548
88.9%
114
6.5 %
97
5.6 %
18
1.0 %
Parallel - Recurrent breakdowns
46
5244
28.2%
207
3.9%
4596
87.6%
412
7.9 %
365
7.0 %
76
1.4 %
England - HRA
82
11414
61.5%
474
4.2%
9859
86.4%
866
7.6 %
764
6.7 %
317
2.8 %
England - Edge
Parallel New Infection
15
1802
9.7%
81
4.5%
1590
88.2%
144
8.0 %
131
7.3 %
0
Parallel - new infection cull area
2
1180
6.4%
25
2.1%
1102
93.4%
65
5.5 %
53
4.5 %
0
Parallel Persistent Infection
2
160
0.9%
15
9.4%
136
85.0%
9
5.6 %
9
5.6 %
0
Parallel - Recurrent breakdowns
1
114
0.6%
1
0.9%
93
81.6%
11
9.6 %
20
17.5 %
0
England - Edge
20
3256
17.5%
122
3.7%
2921
89.7%
229
7.0 %
213
6.5 %
0
England - LRA
Parallel New Infection
5
403
2.2%
13
3.2%
351
87.1%
29
7.2 %
26
6.5 %
13
3.2 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
England - LRA
6
404
2.2%
13
3.2%
352
87.1%
29
7.2 %
26
6.4 %
13
3.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
11
617
3.3%
80
13.0%
462
74.9%
50
8.1 %
45
7.3 %
31
5.0 %
Parallel - IRs in Exit Strategy for persistent her
6
19
0.1%
3
15.8%
16
84.2%
1
5.3 %
0
0
Parallel - Other
5
163
0.9%
9
5.5%
137
84.0%
18
11.0 %
17
10.4 %
0
Parallel Persistent Infection
3
169
0.9%
5
3.0%
149
88.2%
16
9.5 %
15
8.9 %
0
Parallel - Rapid Testing Two-times IRs
24
72
0.4%
10
13.9%
50
69.4%
3
4.2 %
3
4.2 %
9
12.5 %
Parallel - Recurrent breakdowns
13
359
1.9%
26
7.2%
318
88.6%
21
5.8 %
12
3.3 %
3
0.8 %
Parallel - severe IRs - in persistent herds
26
111
0.6%
8
7.2%
76
68.5%
9
8.1 %
7
6.3 %
20
18.0 %
Wales - High TB
88
1510
8.1%
141
9.3%
1208
80.0%
118
7.8 %
99
6.6 %
63
4.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
397
2.1%
33
8.3%
347
87.4%
17
4.3 %
16
4.0 %
1
0.3 %
Parallel - IRs in Exit Strategy for persistent her
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
4
352
1.9%
4
1.1%
334
94.9%
12
3.4 %
12
3.4 %
2
0.6 %
Parallel - Other
3
216
1.2%
8
3.7%
182
84.3%
28
13.0 %
24
11.1 %
2
0.9 %
Parallel Persistent Infection
3
191
1.0%
3
1.6%
169
88.5%
18
9.4 %
18
9.4 %
1
0.5 %
Parallel – post-mvt test coupled with IDEXX an
5
11
0.1%
0
0.0 %
11
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
7
18
0.1%
7
38.9%
11
61.1%
0
0
0
Parallel - Recurrent breakdowns
1
107
0.6%
2
1.9%
99
92.5%
6
5.6 %
6
5.6 %
0
Parallel - severe IRs - in persistent herds
4
17
0.1%
2
11.8%
11
64.7%
0
0
4
23.5 %
Wales - Intermediate TB
36
1313
7.1%
60
4.6%
1167
88.9%
81
6.2 %
76
5.8 %
10
0.8 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
384
2.1%
38
9.9%
312
81.3%
40
10.4 %
34
8.9 %
0
Parallel - Herd or Group Slaughter
1
34
0.2%
2
5.9%
26
76.5%
6
17.6 %
6
17.6 %
0
Parallel New Infection
6
192
1.0%
1
0.5%
170
88.5%
24
12.5 %
19
9.9 %
2
1.0 %
Parallel – post-mvt test coupled with IDEXX an
19
46
0.2%
1
2.2%
44
95.7%
1
2.2 %
1
2.2 %
0
Parallel - Rapid Testing Two-times IRs
1
8
0.0%
0
0.0 %
7
87.5%
1
12.5 %
1
12.5 %
0
Wales - Low TB
29
664
3.6%
42
6.3%
559
84.2%
72
10.8 %
61
9.2 %
2
0.3 %
Scotland - Scotland
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Scotland - Scotland
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
1398
7.5%
151
10.8%
1121
80.2%
107
7.7 %
95
6.8 %
32
2.3 %
Parallel - Herd or Group Slaughter
1
34
0.2%
2
5.9%
26
76.5%
6
17.6 %
6
17.6 %
0
Parallel - IRs in Exit Strategy for persistent herds
8
23
0.1%
4
17.4%
19
82.6%
1
4.3 %
0
0
Parallel New Infection
31
2768
14.9%
100
3.6%
2458
88.8%
214
7.7 %
193
7.0 %
17
0.6 %
Parallel - new infection cull area
15
2389
12.9%
87
3.6%
2171
90.9%
148
6.2 %
129
5.4 %
2
0.1 %
Parallel - Other
10
591
3.2%
21
3.6%
521
88.2%
53
9.0 %
47
8.0 %
2
0.3 %
Parallel Persistent Infection
22
3508
18.9%
144
4.1%
2885
82.2%
288
8.2 %
257
7.3 %
222
6.3 %
Parallel - persistent infection cull area
6
1742
9.4%
79
4.5%
1548
88.9%
114
6.5 %
97
5.6 %
18
1.0 %
Parallel – post-mvt test coupled with IDEXX anti
24
57
0.3%
1
1.8%
55
96.5%
1
1.8 %
1
1.8 %
0
Parallel - Rapid Testing Two-times IRs
32
98
0.5%
17
17.3%
68
69.4%
4
4.1 %
4
4.1 %
9
9.2 %
Parallel - Recurrent breakdowns
61
5824
31.4%
236
4.1%
5106
87.7%
450
7.7 %
403
6.9 %
79
1.4 %
Parallel - severe IRs - in persistent herds
30
128
0.7%
10
7.8%
87
68.0%
9
7.0 %
7
5.5 %
24
18.8 %
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Total
262
18563
100.0%
852
4.6%
16068
86.6%
1395
7.5 %
1239
6.7 %
405
2.2 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2022)
England - HRA
Parallel New Infection
1
19
0.1%
1
5.3%
13
68.4%
5
26.3 %
5
26.3 %
0
Parallel - new infection cull area
13
1209
6.5%
62
5.1%
1069
88.4%
83
6.9 %
76
6.3 %
2
0.2 %
Parallel - Other
2
212
1.1%
4
1.9%
202
95.3%
7
3.3 %
6
2.8 %
0
Parallel Persistent Infection
14
2988
16.1%
121
4.0%
2431
81.4%
245
8.2 %
215
7.2 %
221
7.4 %
Parallel - persistent infection cull area
6
1742
9.4%
79
4.5%
1548
88.9%
114
6.5 %
97
5.6 %
18
1.0 %
Parallel - Recurrent breakdowns
46
5244
28.2%
207
3.9%
4596
87.6%
412
7.9 %
365
7.0 %
76
1.4 %
England - Edge
Parallel New Infection
15
1802
9.7%
81
4.5%
1590
88.2%
144
8.0 %
131
7.3 %
0
Parallel - new infection cull area
2
1180
6.4%
25
2.1%
1102
93.4%
65
5.5 %
53
4.5 %
0
Parallel Persistent Infection
2
160
0.9%
15
9.4%
136
85.0%
9
5.6 %
9
5.6 %
0
Parallel - Recurrent breakdowns
1
114
0.6%
1
0.9%
93
81.6%
11
9.6 %
20
17.5 %
0
England - LRA
Parallel New Infection
5
403
2.2%
13
3.2%
351
87.1%
29
7.2 %
26
6.5 %
13
3.2 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
England
108
15074
81.2%
609
4.0%
13132
87.1%
1124
7.5 %
1003
6.7 %
330
2.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
11
617
3.3%
80
13.0%
462
74.9%
50
8.1 %
45
7.3 %
31
5.0 %
Parallel - IRs in Exit Strategy for persistent herd
6
19
0.1%
3
15.8%
16
84.2%
1
5.3 %
0
0
Parallel - Other
5
163
0.9%
9
5.5%
137
84.0%
18
11.0 %
17
10.4 %
0
Parallel Persistent Infection
3
169
0.9%
5
3.0%
149
88.2%
16
9.5 %
15
8.9 %
0
Parallel - Rapid Testing Two-times IRs
24
72
0.4%
10
13.9%
50
69.4%
3
4.2 %
3
4.2 %
9
12.5 %
Parallel - Recurrent breakdowns
13
359
1.9%
26
7.2%
318
88.6%
21
5.8 %
12
3.3 %
3
0.8 %
Parallel - severe IRs - in persistent herds
26
111
0.6%
8
7.2%
76
68.5%
9
8.1 %
7
6.3 %
20
18.0 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
397
2.1%
33
8.3%
347
87.4%
17
4.3 %
16
4.0 %
1
0.3 %
Parallel - IRs in Exit Strategy for persistent herd
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
4
352
1.9%
4
1.1%
334
94.9%
12
3.4 %
12
3.4 %
2
0.6 %
Parallel - Other
3
216
1.2%
8
3.7%
182
84.3%
28
13.0 %
24
11.1 %
2
0.9 %
Parallel Persistent Infection
3
191
1.0%
3
1.6%
169
88.5%
18
9.4 %
18
9.4 %
1
0.5 %
Parallel – post-mvt test coupled with IDEXX ant
5
11
0.1%
0
0.0 %
11
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
7
18
0.1%
7
38.9%
11
61.1%
0
0
0
Parallel - Recurrent breakdowns
1
107
0.6%
2
1.9%
99
92.5%
6
5.6 %
6
5.6 %
0
Parallel - severe IRs - in persistent herds
4
17
0.1%
2
11.8%
11
64.7%
0
0
4
23.5 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
384
2.1%
38
9.9%
312
81.3%
40
10.4 %
34
8.9 %
0
Parallel - Herd or Group Slaughter
1
34
0.2%
2
5.9%
26
76.5%
6
17.6 %
6
17.6 %
0
Parallel New Infection
6
192
1.0%
1
0.5%
170
88.5%
24
12.5 %
19
9.9 %
2
1.0 %
Parallel – post-mvt test coupled with IDEXX ant
19
46
0.2%
1
2.2%
44
95.7%
1
2.2 %
1
2.2 %
0
Parallel - Rapid Testing Two-times IRs
1
8
0.0%
0
0.0 %
7
87.5%
1
12.5 %
1
12.5 %
0
Wales
153
3487
18.8%
243
7.0%
2934
84.1%
271
7.8 %
236
6.8 %
75
2.2 %
Scotland - Scotland
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Scotland
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2021 February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
0
84
5
12
March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
4
0
9
0
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
4
0
4
0
1083
0
92
0
6
July
298
24841
164
1077
30
0
0
0
0
61
4
0
4
0
936
0
40
0
2
August
312
17920
167
912
3
26
0
0
0
0
35
2
0
7
0
755
1
81
1
1
September
274
15805
130
890
13
0
0
0
0
95
1
0
7
0
742
0
31
0
0
October
276
19790
140
814
15
0
0
0
0
44
2
2
17
1
696
0
35
1
1
November
329
25737
195
1349
58
0
0
0
0
92
0
1
22
1
1114
10
50
0
1
December
238
13117
138
759
14
0
0
0
0
42
0
8
0
0
656
36
2
0
0
2022 January
262
18563
129
852
2
376
0
0
0
0
16
0
16
0
0
371
10
0
0
0
Totals (last
12 months)
3839
283994
2187
14606
9
717
0
0
0
0
900
30
27
103
2
11948
58
714
9
24
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1930
219518
1256
10593
633
0
0
0
0
781
13
14
55
1
9002
1
75
0
0
Scotland
21
2005
14
149
0
0
0
0
0
47
1
0
6
0
53
0
22
3
17
Wales
1888
62471
917
3864
84
0
0
0
0
72
16
13
42
1
2893
57
617
6
7
Totals
3839
283994
2187
14606
717
0
0
0
0
900
30
27
103
2
11948
58
714
9
24
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2020
2021
2022
IFNg Positive herds
IFNg Negative herds
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2020
2021
2022
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
2020
2021
2022
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
W Yorks
Wiltshire
Staffs
Shropshire
Powys
Oxon
Northants
Leics & Rut
Heref
Gwynedd
Gr Manchester
E Sussex
Derbyshire
Cheshire
Carms
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during January 2022 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
Avon
4.08 %
4.72 %
Bucks
7.69 %
7.69 %
Carms
6.67 %
9.33 %
Ceredigion
3.26 %
4.35 %
Cheshire
4.95 %
5.90 %
Corn & Scilly
7.44 %
7.85 %
Derbyshire
8.33 %
8.89 %
Devon
7.44 %
8.58 %
E Sussex
Gloucs
13.28 %
13.28 %
Gr Manchester
6.06 %
6.06 %
Gwent
3.90 %
7.79 %
Gwynedd
13.16 %
14.47 %
Hampshire
Heref
5.39 %
7.09 %
Lancashire
Leics & Rut
3.71 %
3.94 %
NE Wales
6.56 %
7.37 %
Northants
15.40 %
13.89 %
Northumb
6.85 %
7.66 %
Oxon
6.45 %
7.22 %
Pembs
6.10 %
6.96 %
Powys
8.64 %
9.88 %
S Glamorgan
Shropshire
7.24 %
7.66 %
Somerset excl
4.59 %
7.64 %
Staffs
4.19 %
4.95 %
W Glamorgan
5.61 %
5.61 %
Wiltshire
8.13 %
8.96 %
W Sussex
W Yorks
5.79 %
6.61 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
14814
609
4.1%
15074
609
4.0%
Wales
3286
243
7.4%
3487
243
7.0%
Scotland
2
0
0.0 %
2
0.0 %
GB
18102
852
4.7%
18563
852
4.6%
Table 6a (i): Number of animals and submissions tested in January 2022
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
14814
609
4.1%
15074
609
4.0%
Wales
3286
243
7.4%
3487
243
7.0%
Scotland
2
0
0.0 %
2
0.0 %
GB
18102
852
4.7%
18563
852
4.6%
Table 6b (i): Number of animals and submissions tested in 2022 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22822
1354
5.9%
23125
1354
5.9%
Wales
4652
298
6.4%
4795
298
6.2%
Scotland
20
1
5.0%
20
1
5.0%
GB
27493
1653
6.0%
27940
1653
5.9%
Table 6a (ii): Number of animals and submissions tested in January 2021
Table 6b (ii): Number of animals and submissions tested in 2021 Up to end of January
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22822
1354
5.9%
23125
1354
5.9%
Wales
4652
298
6.4%
4795
298
6.2%
Scotland
20
1
5.0%
20
1
5.0%
GB
27493
1653
6.0%
27940
1653
5.9%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Hampshire
PRV-SE
1
7
2
28.57 %
Wiltshire
PRV-SE
1
1
0
0
Total
2
8
2
25.00 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples January 2022
Table 7b Number of private samples 2022
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Hampshire
PRV-SE
1
7
2
28.57 %
Wiltshire
PRV-SE
1
1
0
0
Total
2
8
2
25.00 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
1
19
0
0
Ceredigion
BOV-IR-C
2
48
3
6.3%
North East Wales
BOV-IR-C
6
89
13
14.6%
BOV-OTHER
1
17
0
0
BOV-P
1
420
43
10.2%
BOV-POSTM
8
23
6
26.1%
Pembrokeshire
BOV-IR-C
12
89
18
20.2%
BOV-IR-EX
2
11
2
18.2%
BOV-OTHER
1
16
2
12.5%
BOV-P
1
115
15
13.0%
Powys
BOV-IR-C
2
13
3
23.1%
South Wales
BOV-IR-C
1
4
0
0
Wales
38
864
105
12.2%
All GB
38
864
105
12.2 %
Table 8a Number of Cattle Antibody Tests January 2022
Table 8b Number of Cattle Antibody Tests 2022
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Wales
Carmarthenshire
BOV-IR-C
1
19
0
0
Ceredigion
BOV-IR-C
2
48
3
6.3%
North East Wales
BOV-IR-C
6
89
13
14.6%
BOV-OTHER
1
17
0
0
BOV-P
1
420
43
10.2%
BOV-POSTM
8
23
6
26.1%
Pembrokeshire
BOV-IR-C
12
89
18
20.2%
BOV-IR-EX
2
11
2
18.2%
BOV-OTHER
1
16
2
12.5%
BOV-P
1
115
15
13.0%
Powys
BOV-IR-C
2
13
3
23.1%
South Wales
BOV-IR-C
1
4
0
0
Wales
38
864
105
12.2%
All GB
38
864
105
12.2 %