Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - October 2022
Source: environment.data.gov.uk (2023-01-25)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2022. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 194
Report Period 1st - 31st October 2022
Issued 10 November 2022

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
October
2022
%
Total 2022
%
Submissions
180
2368
Samples
Total (%)
xx-xxxx-xxxx
100.00 %
184578
100.00 %
England
11946
72.81 %
137044
74.25 %
Wales
4374
26.66 %
44218
23.96 %
Scotland
86
0.52 %
3316
1.80 %
Parallel Tests
PAR-CHR
0
0.00 %
2621
1.42 %
PAR-CUL-N
23
0.14 %
8108
4.39 %
PAR-CUL-P
905
5.52 %
6479
3.51 %
PAR-HERD-S
4
0.02 %
467
0.25 %
PAR-IR-EX
42
0.26 %
336
0.18 %
PAR-IR-SEV
109
0.66 %
1728
0.94 %
PAR-NEW-IN
2946
17.96 %
38947
21.10 %
PAR-OTHER
231
1.41 %
3273
1.77 %
PAR-PERSIS
xx-xxxx-xxxx
10.33 %
16636
9.01 %
PAR-POSTM
16
0.10 %
376
0.20 %
PAR-RAPID
67
0.41 %
741
0.40 %
PAR-RECUR
9140
55.71 %
89930
48.72 %
Total(% of all samples)
15177
92.51 %
xx-xxxx-xxxx
91.91 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
1229
7.49 %
14930
8.09 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
2
0.00 %
Total(% of all samples)
1229
7.49 %
14936
8.09 %
Total
xx-xxxx-xxxx
100.00 %
184578
100.00 %
Retests
Total (% of all samples)
1207
7.36 %
14927
8.09 %
England
876
5.34 %
10971
5.94 %
Wales
301
1.83 %
3685
2.00 %
Scotland
30
0.18 %
271
0.15 %
Resamples
Total (% of all samples)
904
5.51 %
11927
6.46 %
England
661
4.03 %
8840
4.79 %
Wales
218
1.33 %
2868
1.55 %
Scotland
25
0.15 %
219
0.12 %
Rejects
Total (% of all samples)
17
0.10 %
986
0.53 %
England
8
0.05 %
803
0.44 %
Wales
9
0.05 %
180
0.10 %
Scotland
0
0.00 %
3
0.00 %
Table 1. Monthly Totals for October 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
23
0.1 %
PAR-CUL-P
905
5.5 %
PAR-HERD-S
4
0.0 %
PAR-IR-EX
42
0.3 %
PAR-IR-SEV
109
0.7 %
PAR-NEW-IN
2946
18.0 %
PAR-OTHER
231
1.4 %
PAR-PERSIS
xx-xxxx-xxxx
10.3 %
PAR-POSTM
16
0.1 %
PAR-RAPID
67
0.4 %
PAR-RECUR
9140
55.7 %
SER-FLEXI
1229
7.5 %
Total
xx-xxxx-xxxx
100.00 %
Figure 1. Number of samples submitted in each category during October 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
200
400
600
800
1000
1200
1400
1600
1800
2000
Wigtown
Ayrshire
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
Wiltshire
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
N Somer S Glos
Leics & Rut
Heref
Gloucs
Devon
Derbyshire
Corn & Scilly
Cheshire
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during October 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
14000
16000
18000
Wigtown
Kirkcudbright
Inverness-shire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
W Sussex
Worcs
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
N Somer S Glos
Notts
Northumb
Northants
Lincs
Leics & Rut
Lancashire
Kent
I of Wight
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Cambs
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
6
1,951
11.89 %
96
4.92 %
1787
91.59 %
88
4.51 %
68
3.49 %
0
0.00 %
Corn & Scilly
PAR-CUL-P
2
905
5.52 %
22
2.43 %
820
90.61 %
81
8.95 %
60
6.63 %
3
0.33 %
PAR-RECUR
2
561
3.42 %
15
2.67 %
515
91.80 %
39
6.95 %
31
5.53 %
0
0.00 %
Devon
PAR-CUL-N
1
19
0.12 %
0
0.00 %
17
89.47 %
2
10.53 %
2
10.53 %
0
0.00 %
PAR-RECUR
2
320
1.95 %
1
0.31 %
297
92.81 %
41
12.81 %
22
6.88 %
0
0.00 %
Gloucs
PAR-RECUR
6
1,449
8.83 %
45
3.11 %
1326
91.51 %
96
6.63 %
77
5.31 %
1
0.07 %
Heref
PAR-RECUR
5
621
3.79 %
27
4.35 %
555
89.37 %
50
8.05 %
39
6.28 %
0
0.00 %
N Somer S Glos
PAR-RECUR
1
3
0.02 %
0
0.00 %
1
33.33 %
2
66.67 %
2
66.67 %
0
0.00 %
Shropshire
PAR-PERSIS
7
1,200
7.31 %
49
4.08 %
1100
91.67 %
73
6.08 %
50
4.17 %
1
0.08 %
PAR-RECUR
4
696
4.24 %
20
2.87 %
647
92.96 %
48
6.90 %
29
4.17 %
0
0.00 %
Somerset excl N
PAR-PERSIS
2
233
1.42 %
14
6.01 %
212
90.99 %
9
3.86 %
7
3.00 %
0
0.00 %
PAR-RECUR
6
821
5.00 %
34
4.14 %
748
91.11 %
49
5.97 %
39
4.75 %
0
0.00 %
Staffs
PAR-RECUR
8
1,516
9.24 %
24
1.58 %
1382
91.16 %
123
8.11 %
109
7.19 %
1
0.07 %
Wiltshire
PAR-OTHER
2
84
0.51 %
12
14.29 %
57
67.86 %
16
19.05 %
15
17.86 %
0
0.00 %
PAR-RECUR
3
37
0.23 %
0
0.00 %
32
86.49 %
8
21.62 %
5
13.51 %
0
0.00 %
England - Edge
Cheshire
PAR-CUL-N
1
4
0.02 %
0
0.00 %
1
25.00 %
3
75.00 %
3
75.00 %
0
0.00 %
PAR-OTHER
1
106
0.65 %
11
10.38 %
90
84.91 %
7
6.60 %
5
4.72 %
0
0.00 %
Derbyshire
PAR-NEW-IN
2
621
3.79 %
7
1.13 %
578
93.08 %
52
8.37 %
36
5.80 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
1
159
0.97 %
9
5.66 %
137
86.16 %
19
11.95 %
13
8.18 %
0
0.00 %
Oxon
PAR-NEW-IN
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
138
0.84 %
1
0.72 %
122
88.41 %
17
12.32 %
13
9.42 %
2
1.45 %
England - LRA
N Yorks
PAR-NEW-IN
2
94
0.57 %
4
4.26 %
87
92.55 %
4
4.26 %
3
3.19 %
0
0.00 %
Surrey
PAR-NEW-IN
2
311
1.90 %
4
1.29 %
279
89.71 %
37
11.90 %
28
9.00 %
0
0.00 %
W Sussex
PAR-NEW-IN
1
95
0.58 %
7
7.37 %
83
87.37 %
12
12.63 %
5
5.26 %
0
0.00 %
England
69
11946
72.81 %
402
3.37 %
10875
91.03 %
876
7.33 %
661
5.53 %
8
0.07 %
Wales - High TB
Carms
PAR-IR-EX
1
6
0.04 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Table 2a. Summary by County for October 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
4
44
0.27 %
10
22.73 %
33
75.00 %
1
2.27 %
1
2.27 %
0
0.00 %
PAR-RAPID
7
17
0.10 %
0
0.00 %
17
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
1,005
6.13 %
29
2.89 %
926
92.14 %
66
6.57 %
49
4.88 %
1
0.10 %
SER-FLEXI
2
131
0.80 %
17
12.98 %
105
80.15 %
7
5.34 %
6
4.58 %
3
2.29 %
Ceredigion
PAR-IR-EX
2
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
12
0.07 %
0
0.00 %
11
91.67 %
2
16.67 %
1
8.33 %
0
0.00 %
PAR-PERSIS
2
131
0.80 %
3
2.29 %
124
94.66 %
4
3.05 %
4
3.05 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
102
0.62 %
1
0.98 %
95
93.14 %
7
6.86 %
6
5.88 %
0
0.00 %
Gwent
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
5
0.03 %
1
20.00 %
2
40.00 %
3
60.00 %
2
40.00 %
0
0.00 %
PAR-RECUR
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Pembs
PAR-IR-EX
3
14
0.09 %
8
57.14 %
6
42.86 %
1
7.14 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
11
45
0.27 %
10
22.22 %
30
66.67 %
5
11.11 %
5
11.11 %
0
0.00 %
PAR-PERSIS
1
8
0.05 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
PAR-RAPID
7
32
0.20 %
5
15.63 %
27
84.38 %
1
3.13 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
203
1.24 %
8
3.94 %
178
87.68 %
21
10.34 %
16
7.88 %
1
0.49 %
Powys
PAR-IR-EX
2
16
0.10 %
4
25.00 %
12
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
4
0.02 %
1
25.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RECUR
1
5
0.03 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
SER-FLEXI
3
416
2.54 %
24
5.77 %
369
88.70 %
31
7.45 %
23
5.53 %
0
0.00 %
S Wales
PAR-IR-SEV
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RECUR
1
16
0.10 %
1
6.25 %
13
81.25 %
2
12.50 %
2
12.50 %
0
0.00 %
Ceredigion
SER-FLEXI
1
377
2.30 %
14
3.71 %
352
93.37 %
14
3.71 %
9
2.39 %
2
0.53 %
NE Wales
PAR-HERD-S
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
991
6.04 %
50
5.05 %
896
90.41 %
64
6.46 %
43
4.34 %
2
0.20 %
PAR-PERSIS
2
122
0.74 %
8
6.56 %
105
86.07 %
11
9.02 %
9
7.38 %
0
0.00 %
PAR-POSTM
2
14
0.09 %
0
0.00 %
14
100.00 %
1
7.14 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-POSTM
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
3
7
0.04 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
5
505
3.08 %
8
1.58 %
468
92.67 %
40
7.92 %
29
5.74 %
0
0.00 %
PAR-POSTM
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
4
81
0.49 %
5
6.17 %
73
90.12 %
6
7.41 %
3
3.70 %
0
0.00 %
PAR-OTHER
2
41
0.25 %
0
0.00 %
38
92.68 %
5
12.20 %
3
7.32 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
Wales
109
4374
26.66 %
213
4.87 %
3934
89.94 %
301
6.88 %
218
4.98 %
9
0.21 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
1
2
0.01 %
1
50.00 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
Wigtown
PAR-NEW-IN
1
84
0.51 %
4
4.76 %
56
66.67 %
28
33.33 %
24
28.57 %
0
0.00 %
Scotland
2
86
0.52 %
5
5.81 %
56
65.12 %
30
34.88 %
25
29.07 %
0
0.00 %
Grand Total
Sum:
180
xx-xxxx-xxxx
620
14865
1207
904
17
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
4
257
0.14 %
22
8.56 %
212
82.49 %
27
10.51 %
23
8.95 %
0
0.00 %
PAR-CUL-N
3
527
0.29 %
26
4.93 %
485
92.03 %
16
3.04 %
15
2.85 %
1
0.19 %
PAR-CUL-P
2
222
0.12 %
20
9.01 %
173
77.93 %
37
16.67 %
29
13.06 %
0
0.00 %
PAR-NEW-IN
1
77
0.04 %
2
2.60 %
64
83.12 %
16
20.78 %
11
14.29 %
0
0.00 %
PAR-OTHER
2
54
0.03 %
11
20.37 %
35
64.81 %
9
16.67 %
8
14.81 %
0
0.00 %
PAR-PERSIS
2
343
0.19 %
20
5.83 %
301
87.76 %
26
7.58 %
22
6.41 %
0
0.00 %
PAR-RECUR
29
4,441
2.41 %
322
7.25 %
3902
87.86 %
281
6.33 %
208
4.68 %
9
0.20 %
Corn & Scilly
PAR-CHR
2
197
0.11 %
9
4.57 %
166
84.26 %
22
11.17 %
22
11.17 %
0
0.00 %
PAR-CUL-N
7
595
0.32 %
54
9.08 %
520
87.39 %
32
5.38 %
21
3.53 %
0
0.00 %
PAR-CUL-P
7
1,589
0.86 %
49
3.08 %
1429
89.93 %
135
8.50 %
108
6.80 %
3
0.19 %
PAR-OTHER
1
102
0.06 %
1
0.98 %
98
96.08 %
4
3.92 %
3
2.94 %
0
0.00 %
PAR-PERSIS
10
1,862
1.01 %
73
3.92 %
1656
88.94 %
160
8.59 %
133
7.14 %
0
0.00 %
PAR-RECUR
81
12,045
6.53 %
479
3.98 %
10582
87.85 %
1171
9.72 %
978
8.12 %
6
0.05 %
Devon
PAR-CHR
6
496
0.27 %
6
1.21 %
446
89.92 %
51
10.28 %
44
8.87 %
0
0.00 %
PAR-CUL-N
20
1,897
1.03 %
116
6.11 %
1600
84.34 %
198
10.44 %
174
9.17 %
7
0.37 %
PAR-CUL-P
7
1,197
0.65 %
47
3.93 %
1051
87.80 %
114
9.52 %
94
7.85 %
5
0.42 %
PAR-HERD-S
2
288
0.16 %
50
17.36 %
225
78.13 %
27
9.38 %
13
4.51 %
0
0.00 %
PAR-OTHER
1
9
0.00 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-PERSIS
13
2,145
1.16 %
87
4.06 %
1909
89.00 %
179
8.34 %
146
6.81 %
3
0.14 %
PAR-RECUR
85
11,587
6.28 %
533
4.60 %
10162
87.70 %
1078
9.30 %
876
7.56 %
16
0.14 %
SER-FLEXI
2
145
0.08 %
4
2.76 %
128
88.28 %
11
7.59 %
9
6.21 %
4
2.76 %
Dorset
PAR-CUL-N
5
1,423
0.77 %
58
4.08 %
1279
89.88 %
100
7.03 %
83
5.83 %
3
0.21 %
PAR-OTHER
4
71
0.04 %
1
1.41 %
68
95.77 %
2
2.82 %
2
2.82 %
0
0.00 %
PAR-RECUR
13
1,718
0.93 %
73
4.25 %
1524
88.71 %
147
8.56 %
121
7.04 %
0
0.00 %
SER-FLEXI
2
43
0.02 %
7
16.28 %
34
79.07 %
2
4.65 %
2
4.65 %
0
0.00 %
Gloucs
PAR-CUL-N
7
320
0.17 %
23
7.19 %
247
77.19 %
51
15.94 %
47
14.69 %
3
0.94 %
Table 2b. Summary by County for 2022
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
1
195
0.11 %
7
3.59 %
170
87.18 %
17
8.72 %
17
8.72 %
1
0.51 %
PAR-OTHER
2
64
0.03 %
2
3.13 %
56
87.50 %
7
10.94 %
6
9.38 %
0
0.00 %
PAR-RECUR
36
5,059
2.74 %
193
3.81 %
4550
89.94 %
383
7.57 %
311
6.15 %
5
0.10 %
Heref
PAR-CUL-P
2
625
0.34 %
32
5.12 %
570
91.20 %
35
5.60 %
23
3.68 %
0
0.00 %
PAR-HERD-S
2
69
0.04 %
13
18.84 %
48
69.57 %
13
18.84 %
8
11.59 %
0
0.00 %
PAR-OTHER
6
545
0.30 %
32
5.87 %
488
89.54 %
42
7.71 %
25
4.59 %
0
0.00 %
PAR-PERSIS
9
1,132
0.61 %
61
5.39 %
975
86.13 %
104
9.19 %
93
8.22 %
3
0.27 %
PAR-RECUR
61
7,733
4.19 %
267
3.45 %
6902
89.25 %
569
7.36 %
433
5.60 %
131
1.69 %
N Somer S Glos
PAR-RECUR
2
61
0.03 %
2
3.28 %
54
88.52 %
11
18.03 %
5
8.20 %
0
0.00 %
Shropshire
PAR-CHR
6
1,235
0.67 %
45
3.64 %
1125
91.09 %
81
6.56 %
65
5.26 %
0
0.00 %
PAR-NEW-IN
2
293
0.16 %
22
7.51 %
252
86.01 %
27
9.22 %
19
6.48 %
0
0.00 %
PAR-OTHER
3
246
0.13 %
4
1.63 %
221
89.84 %
22
8.94 %
21
8.54 %
0
0.00 %
PAR-PERSIS
17
3,264
1.77 %
126
3.86 %
2940
90.07 %
258
7.90 %
197
6.04 %
1
0.03 %
PAR-RECUR
73
12,133
6.57 %
252
2.08 %
11229
92.55 %
800
6.59 %
646
5.32 %
6
0.05 %
Somerset excl N
PAR-CUL-N
2
529
0.29 %
12
2.27 %
492
93.01 %
29
5.48 %
25
4.73 %
0
0.00 %
PAR-OTHER
3
87
0.05 %
15
17.24 %
65
74.71 %
8
9.20 %
7
8.05 %
0
0.00 %
PAR-PERSIS
5
472
0.26 %
18
3.81 %
427
90.47 %
33
6.99 %
27
5.72 %
0
0.00 %
PAR-RECUR
57
8,595
4.66 %
341
3.97 %
7829
91.09 %
502
5.84 %
379
4.41 %
46
0.54 %
SER-FLEXI
4
64
0.03 %
9
14.06 %
49
76.56 %
7
10.94 %
6
9.38 %
0
0.00 %
Staffs
PAR-CHR
2
436
0.24 %
4
0.92 %
419
96.10 %
19
4.36 %
13
2.98 %
0
0.00 %
PAR-CUL-N
2
263
0.14 %
6
2.28 %
214
81.37 %
43
16.35 %
43
16.35 %
0
0.00 %
PAR-CUL-P
2
426
0.23 %
11
2.58 %
402
94.37 %
17
3.99 %
13
3.05 %
0
0.00 %
PAR-OTHER
5
426
0.23 %
13
3.05 %
396
92.96 %
17
3.99 %
16
3.76 %
1
0.23 %
PAR-PERSIS
5
794
0.43 %
13
1.64 %
482
60.71 %
80
10.08 %
78
9.82 %
221
27.83 %
PAR-RECUR
46
9,954
5.39 %
288
2.89 %
9006
90.48 %
715
7.18 %
582
5.85 %
78
0.78 %
Wiltshire
PAR-CUL-N
4
312
0.17 %
20
6.41 %
267
85.58 %
27
8.65 %
25
8.01 %
0
0.00 %
PAR-CUL-P
4
727
0.39 %
53
7.29 %
609
83.77 %
54
7.43 %
50
6.88 %
15
2.06 %
PAR-NEW-IN
1
19
0.01 %
1
5.26 %
13
68.42 %
5
26.32 %
5
26.32 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
4
178
0.10 %
22
12.36 %
137
76.97 %
24
13.48 %
19
10.67 %
0
0.00 %
PAR-PERSIS
2
70
0.04 %
7
10.00 %
46
65.71 %
20
28.57 %
17
24.29 %
0
0.00 %
PAR-RECUR
25
3,426
1.86 %
138
4.03 %
2996
87.45 %
406
11.85 %
290
8.46 %
2
0.06 %
Worcs
PAR-RECUR
8
392
0.21 %
28
7.14 %
324
82.65 %
49
12.50 %
40
10.20 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
2
148
0.08 %
0
0.00 %
95
64.19 %
14
9.46 %
9
6.08 %
44
29.73 %
PAR-RECUR
2
201
0.11 %
3
1.49 %
187
93.03 %
13
6.47 %
11
5.47 %
0
0.00 %
Bucks
PAR-CUL-N
1
224
0.12 %
10
4.46 %
204
91.07 %
11
4.91 %
10
4.46 %
0
0.00 %
PAR-NEW-IN
12
1,604
0.87 %
30
1.87 %
1486
92.64 %
116
7.23 %
88
5.49 %
0
0.00 %
Cheshire
PAR-CUL-N
5
1,323
0.72 %
34
2.57 %
1224
92.52 %
79
5.97 %
65
4.91 %
0
0.00 %
PAR-CUL-P
7
1,426
0.77 %
12
0.84 %
1337
93.76 %
102
7.15 %
77
5.40 %
0
0.00 %
PAR-NEW-IN
4
555
0.30 %
18
3.24 %
499
89.91 %
42
7.57 %
38
6.85 %
0
0.00 %
PAR-OTHER
4
265
0.14 %
26
9.81 %
220
83.02 %
22
8.30 %
18
6.79 %
1
0.38 %
PAR-PERSIS
8
2,341
1.27 %
57
2.43 %
2166
92.52 %
168
7.18 %
115
4.91 %
3
0.13 %
PAR-RECUR
14
4,562
2.47 %
89
1.95 %
4256
93.29 %
261
5.72 %
216
4.73 %
1
0.02 %
Derbyshire
PAR-NEW-IN
7
1,171
0.63 %
21
1.79 %
1061
90.61 %
109
9.31 %
87
7.43 %
2
0.17 %
PAR-RECUR
10
1,832
0.99 %
31
1.69 %
1716
93.67 %
105
5.73 %
79
4.31 %
6
0.33 %
E Sussex
PAR-NEW-IN
7
459
0.25 %
10
2.18 %
394
85.84 %
61
13.29 %
45
9.80 %
10
2.18 %
Hampshire
PAR-CUL-N
2
60
0.03 %
2
3.33 %
46
76.67 %
12
20.00 %
12
20.00 %
0
0.00 %
PAR-NEW-IN
7
670
0.36 %
21
3.13 %
601
89.70 %
52
7.76 %
45
6.72 %
3
0.45 %
Leics & Rut
PAR-CUL-N
2
31
0.02 %
3
9.68 %
27
87.10 %
2
6.45 %
1
3.23 %
0
0.00 %
PAR-CUL-P
1
72
0.04 %
1
1.39 %
59
81.94 %
15
20.83 %
11
15.28 %
1
1.39 %
PAR-NEW-IN
22
2,677
1.45 %
124
4.63 %
2352
87.86 %
229
8.55 %
196
7.32 %
5
0.19 %
Northants
PAR-NEW-IN
17
1,466
0.79 %
28
1.91 %
1268
86.49 %
182
12.41 %
168
11.46 %
2
0.14 %
PAR-RECUR
1
114
0.06 %
1
0.88 %
93
81.58 %
11
9.65 %
20
17.54 %
0
0.00 %
Notts
PAR-CUL-N
2
451
0.24 %
9
2.00 %
422
93.57 %
31
6.87 %
19
4.21 %
1
0.22 %
PAR-NEW-IN
8
780
0.42 %
24
3.08 %
706
90.51 %
63
8.08 %
50
6.41 %
0
0.00 %
Oxon
PAR-NEW-IN
23
3,246
1.76 %
141
4.34 %
2924
90.08 %
223
6.87 %
180
5.55 %
1
0.03 %
PAR-PERSIS
2
160
0.09 %
15
9.38 %
136
85.00 %
9
5.63 %
9
5.63 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
6
456
0.25 %
8
1.75 %
398
87.28 %
56
12.28 %
48
10.53 %
2
0.44 %
Warks
PAR-NEW-IN
4
176
0.10 %
13
7.39 %
145
82.39 %
22
12.50 %
18
10.23 %
0
0.00 %
PAR-RECUR
5
512
0.28 %
15
2.93 %
455
88.87 %
49
9.57 %
42
8.20 %
0
0.00 %
England - LRA
Beds
PAR-CUL-N
1
153
0.08 %
1
0.65 %
126
82.35 %
32
20.92 %
24
15.69 %
2
1.31 %
PAR-NEW-IN
2
94
0.05 %
1
1.06 %
89
94.68 %
3
3.19 %
3
3.19 %
1
1.06 %
Cambs
PAR-NEW-IN
2
73
0.04 %
1
1.37 %
67
91.78 %
4
5.48 %
4
5.48 %
1
1.37 %
Cleveland
PAR-NEW-IN
2
45
0.02 %
0
0.00 %
43
95.56 %
3
6.67 %
1
2.22 %
1
2.22 %
Cumbria
PAR-NEW-IN
8
809
0.44 %
9
1.11 %
725
89.62 %
73
9.02 %
56
6.92 %
19
2.35 %
Gr Manchester
PAR-NEW-IN
7
303
0.16 %
2
0.66 %
278
91.75 %
27
8.91 %
23
7.59 %
0
0.00 %
Herts
PAR-NEW-IN
5
253
0.14 %
14
5.53 %
197
77.87 %
41
16.21 %
39
15.42 %
3
1.19 %
I of Wight
PAR-NEW-IN
1
86
0.05 %
1
1.16 %
80
93.02 %
7
8.14 %
5
5.81 %
0
0.00 %
Kent
PAR-NEW-IN
1
63
0.03 %
2
3.17 %
56
88.89 %
5
7.94 %
5
7.94 %
0
0.00 %
Lancashire
PAR-NEW-IN
9
1,783
0.97 %
27
1.51 %
1660
93.10 %
114
6.39 %
92
5.16 %
4
0.22 %
SER-NSR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Lincs
PAR-NEW-IN
14
1,060
0.57 %
19
1.79 %
971
91.60 %
95
8.96 %
68
6.42 %
2
0.19 %
Northumb
PAR-NEW-IN
1
248
0.13 %
10
4.03 %
208
83.87 %
19
7.66 %
17
6.85 %
13
5.24 %
N Yorks
PAR-NEW-IN
9
831
0.45 %
16
1.93 %
650
78.22 %
75
9.03 %
61
7.34 %
104
12.52 %
Surrey
PAR-NEW-IN
2
311
0.17 %
4
1.29 %
279
89.71 %
37
11.90 %
28
9.00 %
0
0.00 %
W Sussex
PAR-NEW-IN
2
96
0.05 %
7
7.29 %
84
87.50 %
12
12.50 %
5
5.21 %
0
0.00 %
W Yorks
PAR-NEW-IN
6
379
0.21 %
11
2.90 %
332
87.60 %
44
11.61 %
35
9.23 %
1
0.26 %
England
979
137044
74.25 %
5022
3.66 %
122379
89.30 %
10971
8.01 %
8840
6.45 %
803
0.59 %
Wales - High TB
Carms
PAR-IR-EX
8
31
0.02 %
11
35.48 %
20
64.52 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
36
254
0.14 %
84
33.07 %
140
55.12 %
23
9.06 %
17
6.69 %
13
5.12 %
PAR-OTHER
5
11
0.01 %
0
0.00 %
9
81.82 %
2
18.18 %
2
18.18 %
0
0.00 %
PAR-PERSIS
8
872
0.47 %
35
4.01 %
793
90.94 %
57
6.54 %
41
4.70 %
3
0.34 %
PAR-RAPID
46
156
0.08 %
41
26.28 %
98
62.82 %
13
8.33 %
11
7.05 %
6
3.85 %
PAR-RECUR
16
2,032
1.10 %
83
4.08 %
1860
91.54 %
127
6.25 %
89
4.38 %
1
0.05 %
SER-FLEXI
24
2,919
1.58 %
177
6.06 %
2576
88.25 %
219
7.50 %
160
5.48 %
6
0.21 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Ceredigion
PAR-IR-EX
11
31
0.02 %
7
22.58 %
22
70.97 %
1
3.23 %
1
3.23 %
1
3.23 %
PAR-IR-SEV
29
146
0.08 %
28
19.18 %
108
73.97 %
10
6.85 %
8
5.48 %
2
1.37 %
PAR-PERSIS
3
139
0.08 %
4
2.88 %
131
94.24 %
4
2.88 %
4
2.88 %
0
0.00 %
PAR-RAPID
18
40
0.02 %
12
30.00 %
20
50.00 %
5
12.50 %
5
12.50 %
3
7.50 %
PAR-RECUR
2
32
0.02 %
10
31.25 %
21
65.63 %
1
3.13 %
1
3.13 %
0
0.00 %
SER-FLEXI
5
334
0.18 %
9
2.69 %
310
92.81 %
18
5.39 %
15
4.49 %
0
0.00 %
Gwent
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
15
78
0.04 %
19
24.36 %
56
71.79 %
4
5.13 %
3
3.85 %
0
0.00 %
PAR-OTHER
9
168
0.09 %
7
4.17 %
147
87.50 %
19
11.31 %
14
8.33 %
0
0.00 %
PAR-RAPID
18
43
0.02 %
8
18.60 %
30
69.77 %
7
16.28 %
5
11.63 %
0
0.00 %
PAR-RECUR
13
398
0.22 %
12
3.02 %
359
90.20 %
36
9.05 %
24
6.03 %
3
0.75 %
SER-FLEXI
13
1,328
0.72 %
64
4.82 %
1190
89.61 %
81
6.10 %
70
5.27 %
4
0.30 %
Pembs
PAR-IR-EX
37
165
0.09 %
64
38.79 %
97
58.79 %
7
4.24 %
4
2.42 %
0
0.00 %
PAR-IR-SEV
134
910
0.49 %
196
21.54 %
674
74.07 %
40
4.40 %
33
3.63 %
7
0.77 %
PAR-NEW-IN
1
13
0.01 %
0
0.00 %
8
61.54 %
7
53.85 %
5
38.46 %
0
0.00 %
PAR-OTHER
10
195
0.11 %
21
10.77 %
159
81.54 %
15
7.69 %
15
7.69 %
0
0.00 %
PAR-PERSIS
28
1,711
0.93 %
81
4.73 %
1467
85.74 %
203
11.86 %
153
8.94 %
10
0.58 %
PAR-RAPID
116
294
0.16 %
70
23.81 %
210
71.43 %
16
5.44 %
13
4.42 %
1
0.34 %
PAR-RECUR
21
1,748
0.95 %
69
3.95 %
1574
90.05 %
118
6.75 %
96
5.49 %
9
0.51 %
SER-FLEXI
75
7,671
4.16 %
560
7.30 %
6590
85.91 %
602
7.85 %
480
6.26 %
42
0.55 %
Powys
PAR-HERD-S
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-EX
15
46
0.02 %
7
15.22 %
38
82.61 %
3
6.52 %
1
2.17 %
0
0.00 %
PAR-IR-SEV
22
80
0.04 %
12
15.00 %
55
68.75 %
17
21.25 %
13
16.25 %
0
0.00 %
PAR-OTHER
9
276
0.15 %
18
6.52 %
239
86.59 %
23
8.33 %
19
6.88 %
0
0.00 %
PAR-PERSIS
4
274
0.15 %
2
0.73 %
252
91.97 %
22
8.03 %
20
7.30 %
0
0.00 %
PAR-POSTM
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
36
83
0.04 %
16
19.28 %
59
71.08 %
10
12.05 %
8
9.64 %
0
0.00 %
PAR-RECUR
18
606
0.33 %
16
2.64 %
553
91.25 %
52
8.58 %
36
5.94 %
1
0.17 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
10
637
0.35 %
31
4.87 %
566
88.85 %
51
8.01 %
38
5.97 %
2
0.31 %
S Wales
PAR-IR-SEV
4
20
0.01 %
7
35.00 %
12
60.00 %
2
10.00 %
1
5.00 %
0
0.00 %
PAR-PERSIS
2
18
0.01 %
7
38.89 %
10
55.56 %
1
5.56 %
1
5.56 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-IR-SEV
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
35
0.02 %
8
22.86 %
25
71.43 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
45
0.02 %
4
8.89 %
37
82.22 %
4
8.89 %
4
8.89 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
4
17
0.01 %
4
23.53 %
7
41.18 %
2
11.76 %
2
11.76 %
4
23.53 %
PAR-OTHER
3
23
0.01 %
0
0.00 %
15
65.22 %
6
26.09 %
6
26.09 %
2
8.70 %
PAR-PERSIS
3
16
0.01 %
0
0.00 %
14
87.50 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-RAPID
4
9
0.00 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
16
0.01 %
1
6.25 %
13
81.25 %
2
12.50 %
2
12.50 %
0
0.00 %
SER-FLEXI
2
145
0.08 %
10
6.90 %
127
87.59 %
9
6.21 %
8
5.52 %
0
0.00 %
Ceredigion
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-POSTM
1
13
0.01 %
1
7.69 %
11
84.62 %
0
0.00 %
0
0.00 %
1
7.69 %
PAR-RAPID
1
5
0.00 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
529
0.29 %
16
3.02 %
488
92.25 %
40
7.56 %
23
4.35 %
2
0.38 %
Mid Glamorgan
PAR-RAPID
3
6
0.00 %
1
16.67 %
3
50.00 %
2
33.33 %
2
33.33 %
0
0.00 %
NE Wales
PAR-HERD-S
2
67
0.04 %
3
4.48 %
59
88.06 %
5
7.46 %
5
7.46 %
0
0.00 %
PAR-IR-EX
12
43
0.02 %
6
13.95 %
34
79.07 %
3
6.98 %
3
6.98 %
0
0.00 %
PAR-IR-SEV
31
205
0.11 %
27
13.17 %
171
83.41 %
6
2.93 %
5
2.44 %
2
0.98 %
PAR-NEW-IN
84
7,747
4.20 %
190
2.45 %
7008
90.46 %
686
8.86 %
526
6.79 %
23
0.30 %
PAR-OTHER
4
72
0.04 %
4
5.56 %
58
80.56 %
18
25.00 %
10
13.89 %
0
0.00 %
PAR-PERSIS
14
1,010
0.55 %
40
3.96 %
916
90.69 %
58
5.74 %
52
5.15 %
2
0.20 %
PAR-POSTM
43
158
0.09 %
12
7.59 %
140
88.61 %
8
5.06 %
6
3.80 %
0
0.00 %
PAR-RAPID
27
53
0.03 %
11
20.75 %
42
79.25 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
12
719
0.39 %
46
6.40 %
634
88.18 %
33
4.59 %
29
4.03 %
10
1.39 %
Powys
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
12
0.01 %
2
16.67 %
10
83.33 %
1
8.33 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
21
1,874
1.02 %
80
4.27 %
1638
87.41 %
202
10.78 %
153
8.16 %
3
0.16 %
PAR-OTHER
4
273
0.15 %
8
2.93 %
232
84.98 %
40
14.65 %
33
12.09 %
0
0.00 %
PAR-PERSIS
1
8
0.00 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-POSTM
5
7
0.00 %
0
0.00 %
5
71.43 %
3
42.86 %
2
28.57 %
0
0.00 %
PAR-RAPID
6
11
0.01 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-OTHER
3
9
0.00 %
3
33.33 %
5
55.56 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-RAPID
9
20
0.01 %
10
50.00 %
10
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
115
0.06 %
26
22.61 %
72
62.61 %
21
18.26 %
17
14.78 %
0
0.00 %
Shropshire
PAR-IR-EX
2
5
0.00 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RECUR
2
113
0.06 %
3
2.65 %
101
89.38 %
9
7.96 %
9
7.96 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-EX
3
11
0.01 %
1
9.09 %
10
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
59
2,900
1.57 %
98
3.38 %
2473
85.28 %
378
13.03 %
325
11.21 %
4
0.14 %
PAR-OTHER
3
8
0.00 %
0
0.00 %
6
75.00 %
1
12.50 %
1
12.50 %
1
12.50 %
PAR-POSTM
33
111
0.06 %
6
5.41 %
102
91.89 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-RAPID
3
5
0.00 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-HERD-S
2
40
0.02 %
4
10.00 %
28
70.00 %
8
20.00 %
8
20.00 %
0
0.00 %
PAR-IR-EX
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
44
3,327
1.80 %
60
1.80 %
3090
92.88 %
239
7.18 %
166
4.99 %
11
0.33 %
PAR-OTHER
5
156
0.08 %
9
5.77 %
131
83.97 %
24
15.38 %
16
10.26 %
0
0.00 %
PAR-PERSIS
1
5
0.00 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-POSTM
47
85
0.05 %
5
5.88 %
75
88.24 %
7
8.24 %
4
4.71 %
1
1.18 %
PAR-RAPID
6
13
0.01 %
4
30.77 %
8
61.54 %
2
15.38 %
1
7.69 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2022 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
396
0.21 %
40
10.10 %
322
81.31 %
40
10.10 %
34
8.59 %
0
0.00 %
Wales - UNKNOWN
Carms
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
1365
44218
23.96 %
2544
5.75 %
38628
87.36 %
3685
8.33 %
2868
6.49 %
180
0.41 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-OTHER
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
1
2
0.00 %
1
50.00 %
0
0.00 %
2
100.00 %
1
50.00 %
0
0.00 %
Inverness-shire
PAR-NEW-IN
2
128
0.07 %
2
1.56 %
111
86.72 %
15
11.72 %
15
11.72 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
4
493
0.27 %
8
1.62 %
432
87.63 %
67
13.59 %
53
10.75 %
0
0.00 %
Wigtown
PAR-NEW-IN
15
2,688
1.46 %
79
2.94 %
2456
91.37 %
187
6.96 %
150
5.58 %
3
0.11 %
Scotland
24
3316
1.80 %
90
2.71 %
3004
90.59 %
271
8.17 %
219
6.60 %
3
0.09 %
Grand Total
Sum:
xx-xxxx-xxxx
7656
xx-xxxx-xxxx
14927
11927
986
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol October 2022
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
69
11946
100.0%
402
3.4%
10875
91.0%
876
7.3%
661
5.5%
8
0.1%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
69
11946
100.0%
402
3.4%
10875
91.0%
876
7.3%
661
5.5%
8
0.1%
Scotland
Parallel
2
86
100.0%
5
5.8%
56
65.1%
30
34.9%
25
29.1%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
86
100.0%
5
5.8%
56
65.1%
30
34.9%
25
29.1%
0
0.0 %
Wales
Parallel
98
3145
71.9%
149
4.7%
2835
90.1%
221
7.0%
158
5.0%
3
0.1%
Serial/Extended
11
1229
28.1%
64
5.2%
1099
89.4%
80
6.5%
60
4.9%
6
0.5%
Total
109
4374
100.0%
213
4.9%
3934
89.9%
301
6.9%
218
5.0%
9
0.2%
GB
Parallel
169
15177
92.5%
556
3.7%
13766
90.7%
1127
7.4%
844
5.6%
11
0.1%
Serial/Extended
11
1229
7.5%
64
5.2%
1099
89.4%
80
6.5%
60
4.9%
6
0.5%
Total
180
xx-xxxx-xxxx
100.0%
620
3.8%
14865
90.6%
1207
7.4%
904
5.5%
17
0.1%
69.00
109.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2022)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
970 136791
99.8%
5002
3.7% 122167
89.3%
10951
8.0%
8823
6.4%
799
0.6%
Serial/Extended
9
253
0.2%
20
7.9%
212
83.8%
20
7.9%
17
6.7%
4
1.6%
Total
979 137044
100.0%
5022
3.7% 122379
89.3%
10971
8.0%
8840
6.5%
803
0.6%
Scotland
Parallel
22
3311
99.8%
90
2.7%
2999
90.6%
271
8.2%
219
6.6%
3
0.1%
Serial/Extended
2
5
0.2%
0
0.0 %
5 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
24
3316
100.0%
90
2.7%
3004
90.6%
271
8.2%
219
6.6%
3
0.1%
Wales
Parallel
1217
29540
66.8%
1591
5.4%
25825
87.4%
2592
8.8%
2011
6.8%
114
0.4%
Serial/Extended
148
14678
33.2%
953
6.5%
12803
87.2%
1093
7.4%
857
5.8%
66
0.4%
Total
1365
44218
100.0%
2544
5.8%
38628
87.4%
3685
8.3%
2868
6.5%
180
0.4%
GB
Parallel
xx-xxxx-xxxx
91.9%
6683
3.9% 150991
89.0%
13814
8.1%
11053
6.5%
916
0.5%
Serial/Extended
159
14936
8.1%
973
6.5%
13020
87.2%
1113
7.5%
874
5.9%
70
0.5%
Total
xx-xxxx-xxxx
100.0%
7656
4.1% xx-xxxx-xxxx
88.9%
14927
8.1%
11927
6.5%
986
0.5%
979.00
#######
24.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
11
1229
7.5%
64
5.2%
1099
89.4%
80
6.5 %
60
4.9 %
6
0.5 %
Parallel - Herd or Group Slaughter
1
4
0.0%
0
0.0 %
3
75.0%
1
25.0 %
1
25.0 %
0
Parallel - IRs in Exit Strategy for persistent her
10
42
0.3%
13
31.0%
29
69.0%
1
2.4 %
0
0
Parallel New Infection
31
2946
18.0%
99
3.4%
2659
90.3%
265
9.0 %
186
6.3 %
2
0.1 %
Parallel - new infection cull area
2
23
0.1%
0
0.0 %
18
78.3%
5
21.7 %
5
21.7 %
0
Parallel - Other
5
231
1.4%
23
10.0%
185
80.1%
28
12.1 %
23
10.0 %
0
Parallel Persistent Infection
14
xx-xxxx-xxxx
10.3%
74
4.4%
1548
91.4%
98
5.8 %
71
4.2 %
1
0.1 %
Parallel - persistent infection cull area
2
905
5.5%
22
2.4%
820
90.6%
81
9.0 %
60
6.6 %
3
0.3 %
Parallel – post-mvt test coupled with IDEXX an
4
16
0.1%
0
0.0 %
15
93.8%
2
12.5 %
1
6.3 %
0
Parallel - Rapid Testing Two-times IRs
23
67
0.4%
9
13.4%
55
82.1%
6
9.0 %
3
4.5 %
0
Parallel - Recurrent breakdowns
53
9140
55.7%
293
3.2%
8355
91.4%
631
6.9 %
487
5.3 %
5
0.1 %
Parallel - severe IRs - in persistent herds
24
109
0.7%
23
21.1%
79
72.5%
9
8.3 %
7
6.4 %
0
Total
180
xx-xxxx-xxxx
100.0%
620
3.8%
14865
90.6%
1207
7.4 %
904
5.5 %
17
0.1 %
Table 4a. Summaryof IFN Gamma results by submission reason (October 2022)
England - HRA
Parallel - new infection cull area
1
19
0.1%
0
0.0 %
17
89.5%
2
10.5 %
2
10.5 %
0
Parallel - Other
2
84
0.5%
12
14.3%
57
67.9%
16
19.0 %
15
17.9 %
0
Parallel Persistent Infection
9
1433
8.7%
63
4.4%
1312
91.6%
82
5.7 %
57
4.0 %
1
0.1 %
Parallel - persistent infection cull area
2
905
5.5%
22
2.4%
820
90.6%
81
9.0 %
60
6.6 %
3
0.3 %
Parallel - Recurrent breakdowns
43
7975
48.6%
262
3.3%
7290
91.4%
544
6.8 %
421
5.3 %
2
0.0 %
England - HRA
57
10416
63.5%
359
3.4%
9496
91.2%
725
7.0 %
555
5.3 %
6
0.1 %
England - Edge
Parallel New Infection
4
782
4.8%
16
2.0%
717
91.7%
71
9.1 %
49
6.3 %
0
Parallel - new infection cull area
1
4
0.0%
0
0.0 %
1
25.0%
3
75.0 %
3
75.0 %
0
Parallel - Other
1
106
0.6%
11
10.4%
90
84.9%
7
6.6 %
5
4.7 %
0
Parallel - Recurrent breakdowns
1
138
0.8%
1
0.7%
122
88.4%
17
12.3 %
13
9.4 %
2
1.4 %
England - Edge
7
1030
6.3%
28
2.7%
930
90.3%
98
9.5 %
70
6.8 %
2
0.2 %
England - LRA
Parallel New Infection
5
500
3.0%
15
3.0%
449
89.8%
53
10.6 %
36
7.2 %
0
England - LRA
5
500
3.0%
15
3.0%
449
89.8%
53
10.6 %
36
7.2 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
10
852
5.2%
50
5.9%
747
87.7%
66
7.7 %
51
6.0 %
4
0.5 %
Parallel - IRs in Exit Strategy for persistent her
9
40
0.2%
13
32.5%
27
67.5%
1
2.5 %
0
0
Parallel Persistent Infection
3
139
0.8%
3
2.2%
131
94.2%
5
3.6 %
5
3.6 %
0
Parallel - Rapid Testing Two-times IRs
17
57
0.3%
6
10.5%
48
84.2%
5
8.8 %
3
5.3 %
0
Parallel - Recurrent breakdowns
8
1011
6.2%
29
2.9%
930
92.0%
68
6.7 %
51
5.0 %
1
0.1 %
Parallel - severe IRs - in persistent herds
23
108
0.7%
22
20.4%
79
73.1%
9
8.3 %
7
6.5 %
0
Wales - High TB
70
2207
13.5%
123
5.6%
1962
88.9%
154
7.0 %
117
5.3 %
5
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
377
2.3%
14
3.7%
352
93.4%
14
3.7 %
9
2.4 %
2
0.5 %
Parallel - Herd or Group Slaughter
1
4
0.0%
0
0.0 %
3
75.0%
1
25.0 %
1
25.0 %
0
Parallel - IRs in Exit Strategy for persistent her
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel New Infection
11
992
6.0%
50
5.0%
896
90.3%
65
6.6 %
44
4.4 %
2
0.2 %
Parallel Persistent Infection
2
122
0.7%
8
6.6%
105
86.1%
11
9.0 %
9
7.4 %
0
Parallel – post-mvt test coupled with IDEXX an
3
15
0.1%
0
0.0 %
14
93.3%
2
13.3 %
1
6.7 %
0
Parallel - Rapid Testing Two-times IRs
4
8
0.0%
2
25.0%
6
75.0%
0
0
0
Parallel - Recurrent breakdowns
1
16
0.1%
1
6.3%
13
81.3%
2
12.5 %
2
12.5 %
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales - Intermediate TB
25
1537
9.4%
76
4.9%
1391
90.5%
95
6.2 %
66
4.3 %
4
0.3 %
Wales - Low TB
Parallel New Infection
9
586
3.6%
13
2.2%
541
92.3%
46
7.8 %
32
5.5 %
0
Parallel - Other
2
41
0.2%
0
0.0 %
38
92.7%
5
12.2 %
3
7.3 %
0
Parallel – post-mvt test coupled with IDEXX an
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
1
50.0%
1
50.0%
1
50.0 %
0
0
Wales - Low TB
14
630
3.8%
14
2.2%
581
92.2%
52
8.3 %
35
5.6 %
0
Scotland - Scotland
Parallel New Infection
2
86
0.5%
5
5.8%
56
65.1%
30
34.9 %
25
29.1 %
0
Scotland - Scotland
2
86
0.5%
5
5.8%
56
65.1%
30
34.9 %
25
29.1 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
156
14930
8.1%
973
6.5%
13014
87.2%
1113
7.5 %
874
5.9 %
70
0.5 %
Parallel – chronic breakdown
20
2621
1.4%
86
3.3%
2368
90.3%
200
7.6 %
167
6.4 %
0
Parallel - Herd or Group Slaughter
10
467
0.3%
71
15.2%
362
77.5%
53
11.3 %
34
7.3 %
0
Parallel - IRs in Exit Strategy for persistent herds
92
336
0.2%
96
28.6%
228
67.9%
16
4.8 %
11
3.3 %
1
0.3 %
Parallel New Infection
419
38947
21.1%
1097
2.8%
34795
89.3%
3503
9.0 %
2795
7.2 %
260
0.7 %
Parallel - new infection cull area
63
8108
4.4%
374
4.6%
7153
88.2%
663
8.2 %
564
7.0 %
17
0.2 %
Parallel - Other
92
3273
1.8%
206
6.3%
2817
86.1%
309
9.4 %
245
7.5 %
5
0.2 %
Parallel Persistent Infection
137
16636
9.0%
648
3.9%
14632
88.0%
1384
8.3 %
1110
6.7 %
246
1.5 %
Parallel - persistent infection cull area
33
6479
3.5%
232
3.6%
5800
89.5%
526
8.1 %
422
6.5 %
25
0.4 %
Parallel – post-mvt test coupled with IDEXX anti
130
376
0.2%
24
6.4%
335
89.1%
23
6.1 %
15
4.0 %
2
0.5 %
Parallel - Rapid Testing Two-times IRs
296
741
0.4%
182
24.6%
504
68.0%
55
7.4 %
45
6.1 %
10
1.3 %
Parallel - Recurrent breakdowns
634
89930
48.7%
3288
3.7%
80758
89.8%
6977
7.8 %
5563
6.2 %
322
0.4 %
Parallel - severe IRs - in persistent herds
283
1728
0.9%
379
21.9%
1239
71.7%
105
6.1 %
82
4.7 %
28
1.6 %
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
2
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
7656
4.1%
xx-xxxx-xxxx
88.9%
14927
8.1 %
11927
6.5 %
986
0.5 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2022)
England - HRA
Extended Flexible IFN-GAMMA test
8
252
0.1%
20
7.9%
211
83.7%
20
7.9 %
17
6.7 %
4
1.6 %
Parallel – chronic breakdown
20
2621
1.4%
86
3.3%
2368
90.3%
200
7.6 %
167
6.4 %
0
Parallel - Herd or Group Slaughter
4
357
0.2%
63
17.6%
273
76.5%
40
11.2 %
21
5.9 %
0
Parallel New Infection
4
389
0.2%
25
6.4%
329
84.6%
48
12.3 %
35
9.0 %
0
Parallel - new infection cull area
50
5866
3.2%
315
5.4%
5104
87.0%
496
8.5 %
433
7.4 %
14
0.2 %
Parallel - Other
31
1782
1.0%
102
5.7%
1571
88.2%
136
7.6 %
108
6.1 %
1
0.1 %
Parallel Persistent Infection
63
10082
5.5%
405
4.0%
8736
86.6%
860
8.5 %
713
7.1 %
228
2.3 %
Parallel - persistent infection cull area
25
4981
2.7%
219
4.4%
4404
88.4%
409
8.2 %
334
6.7 %
24
0.5 %
Parallel - Recurrent breakdowns
516
77144
41.8%
2916
3.8%
69060
89.5%
6112
7.9 %
4869
6.3 %
299
0.4 %
England - Edge
Parallel New Infection
113
12952
7.0%
430
3.3%
11531
89.0%
1113
8.6 %
924
7.1 %
67
0.5 %
Parallel - new infection cull area
12
2089
1.1%
58
2.8%
1923
92.1%
135
6.5 %
107
5.1 %
1
0.0 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
Parallel - Other
4
265
0.1%
26
9.8%
220
83.0%
22
8.3 %
18
6.8 %
1
0.4 %
Parallel Persistent Infection
10
2501
1.4%
72
2.9%
2302
92.0%
177
7.1 %
124
5.0 %
3
0.1 %
Parallel - persistent infection cull area
8
1498
0.8%
13
0.9%
1396
93.2%
117
7.8 %
88
5.9 %
1
0.1 %
Parallel - Recurrent breakdowns
38
7677
4.2%
147
1.9%
7105
92.5%
495
6.4 %
416
5.4 %
9
0.1 %
England - LRA
Parallel New Infection
71
6434
3.5%
124
1.9%
5719
88.9%
559
8.7 %
442
6.9 %
149
2.3 %
Parallel - new infection cull area
1
153
0.1%
1
0.7%
126
82.4%
32
20.9 %
24
15.7 %
2
1.3 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
England
979 137044
74.2%
5022
3.7%
122379
89.3%
10971
8.0 %
8840
6.5 %
803
0.6 %
Wales - High TB
Extended Flexible IFN-GAMMA test
127
12889
7.0%
841
6.5%
11232
87.1%
971
7.5 %
763
5.9 %
54
0.4 %
Parallel - Herd or Group Slaughter
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel - IRs in Exit Strategy for persistent herd
72
274
0.1%
89
32.5%
178
65.0%
11
4.0 %
6
2.2 %
1
0.4 %
Parallel New Infection
1
13
0.0%
0
0.0 %
8
61.5%
7
53.8 %
5
38.5 %
0
Parallel - Other
35
685
0.4%
54
7.9%
579
84.5%
61
8.9 %
52
7.6 %
0
Parallel Persistent Infection
45
3014
1.6%
129
4.3%
2653
88.0%
287
9.5 %
219
7.3 %
13
0.4 %
Parallel – post-mvt test coupled with IDEXX ant
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
237
619
0.3%
148
23.9%
419
67.7%
51
8.2 %
42
6.8 %
10
1.6 %
Parallel - Recurrent breakdowns
72
4861
2.6%
194
4.0%
4404
90.6%
338
7.0 %
250
5.1 %
14
0.3 %
Parallel - severe IRs - in persistent herds
241
1490
0.8%
346
23.2%
1047
70.3%
96
6.4 %
75
5.0 %
22
1.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
18
1393
0.8%
72
5.2%
1249
89.7%
82
5.9 %
60
4.3 %
12
0.9 %
Parallel - Herd or Group Slaughter
2
67
0.0%
3
4.5%
59
88.1%
5
7.5 %
5
7.5 %
0
Parallel - IRs in Exit Strategy for persistent herd
15
49
0.0%
6
12.2%
38
77.6%
5
10.2 %
5
10.2 %
0
Parallel New Infection
105
9621
5.2%
270
2.8%
8646
89.9%
888
9.2 %
679
7.1 %
26
0.3 %
Parallel - Other
14
377
0.2%
15
4.0%
310
82.2%
65
17.2 %
50
13.3 %
2
0.5 %
Parallel Persistent Infection
18
1034
0.6%
40
3.9%
938
90.7%
60
5.8 %
54
5.2 %
2
0.2 %
Parallel – post-mvt test coupled with IDEXX ant
49
178
0.1%
13
7.3%
156
87.6%
11
6.2 %
8
4.5 %
1
0.6 %
Parallel - Rapid Testing Two-times IRs
50
104
0.1%
30
28.8%
72
69.2%
2
1.9 %
2
1.9 %
0
Parallel - Recurrent breakdowns
7
247
0.1%
30
12.1%
189
76.5%
32
13.0 %
28
11.3 %
0
Parallel - severe IRs - in persistent herds
40
236
0.1%
33
14.0%
190
80.5%
9
3.8 %
7
3.0 %
6
2.5 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
3
396
0.2%
40
10.1%
322
81.3%
40
10.1 %
34
8.6 %
0
Parallel - Herd or Group Slaughter
3
41
0.0%
4
9.8%
29
70.7%
8
19.5 %
8
19.5 %
0
Parallel - IRs in Exit Strategy for persistent herd
5
13
0.0%
1
7.7%
12
92.3%
0
0
0
Parallel New Infection
103
6227
3.4%
158
2.5%
5563
89.3%
617
9.9 %
491
7.9 %
15
0.2 %
Parallel - Other
8
164
0.1%
9
5.5%
137
83.5%
25
15.2 %
17
10.4 %
1
0.6 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2022) Cont.
Parallel Persistent Infection
1
5
0.0%
2
40.0%
3
60.0%
0
0
0
Parallel – post-mvt test coupled with IDEXX ant
80
196
0.1%
11
5.6%
177
90.3%
12
6.1 %
7
3.6 %
1
0.5 %
Parallel - Rapid Testing Two-times IRs
9
18
0.0%
4
22.2%
13
72.2%
2
11.1 %
1
5.6 %
0
Parallel - Recurrent breakdowns
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - UNKNOWN
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
1365
44218
24.0%
2544
5.8%
38628
87.4%
3685
8.3 %
2868
6.5 %
180
0.4 %
Scotland - Scotland
Parallel New Infection
22
3311
1.8%
90
2.7%
2999
90.6%
271
8.2 %
219
6.6 %
3
0.1 %
Serial - Other
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Scotland
24
3316
1.8%
90
2.7%
3004
90.6%
271
8.2 %
219
6.6 %
3
0.1 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2021 November
329
25737
195
1349
26
0
0
0
0
92
1
0
26
0
1143
0
61
0
0
December
238
13117
138
759
14
0
0
0
0
42
5
0
1
2
656
0
38
0
0
2022 January
262
18563
129
852
19
0
0
0
0
32
1
0
17
0
745
1
36
0
0
February
229
18198
132
966
1
19
0
0
0
0
84
3
0
3
0
765
0
91
0
0
March
268
23046
165
1108
35
0
0
0
0
60
4
0
14
0
929
0
65
1
0
April
220
15067
130
687
9
0
0
0
0
50
2
0
11
0
563
0
52
0
0
May
269
23949
158
918
2
19
0
0
0
0
62
3
0
9
0
778
1
43
1
0
June
213
17755
114
590
19
0
0
0
0
48
0
1
1
1
460
1
57
2
0
July
248
15402
109
485
5
0
0
0
0
46
0
2
3
1
381
1
46
0
0
August
274
19758
144
758
16
0
0
0
0
36
0
4
11
2
638
17
28
0
5
September
205
xx-xxxx-xxxx
107
672
14
0
0
0
0
100
0
8
0
0
520
29
0
0
0
October
180
xx-xxxx-xxxx
100
620
184
247
0
0
0
0
9
0
0
0
0
98
0
0
0
0
Totals (last
12 months)
2935
223432
1621
9764
187
442
0
0
0
0
661
19
15
96
6
7676
50
517
4
5
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1226
167966
825
6502
368
0
0
0
0
641
9
5
38
3
5304
0
13
0
0
Scotland
32
4173
23
104
2
0
0
0
0
3
0
1
0
0
94
0
3
0
0
Wales
1677
51293
773
3158
72
0
0
0
0
17
10
9
58
3
2278
50
501
4
5
Totals
2935
223432
1621
9764
442
0
0
0
0
661
19
15
96
6
7676
50
517
4
5
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2020
2021
2022
IFNg Positive herds
IFNg Negative herds
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2020
2021
2022
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2020
2021
2022
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
160
W Sussex
Wiltshire
Wigtown
S Wales
Surrey
Staffs
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
N Yorks
N Somer S Glos
NE Wales
Leics & Rut
Heref
Gwynedd
Gwent
Gloucs
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Ayrshire
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during October 2022 (% submission per
County)
County
%Resampled %Retested
Avon
3.49 %
4.51 %
Ayrshire
50.00 %
100.00 %
Carms
4.76 %
6.23 %
Ceredigion
3.19 %
4.31 %
Cheshire
7.27 %
9.09 %
Corn & Scilly
6.21 %
8.19 %
Derbyshire
5.80 %
8.37 %
Devon
7.08 %
12.68 %
Gloucs
5.31 %
6.63 %
Gwent
33.33 %
44.44 %
Gwynedd
5.73 %
7.91 %
Heref
6.28 %
8.05 %
Leics & Rut
8.18 %
11.95 %
NE Wales
4.69 %
7.07 %
N Somer S Glo
66.67 %
66.67 %
N Yorks
3.19 %
4.26 %
Oxon
9.29 %
12.14 %
Pembs
7.28 %
9.60 %
Powys
6.07 %
8.09 %
S Glamorgan
Shropshire
4.17 %
6.38 %
Somerset excl
4.36 %
5.50 %
Staffs
7.19 %
8.11 %
Surrey
9.00 %
11.90 %
S Wales
Wigtown
28.57 %
33.33 %
Wiltshire
16.53 %
19.83 %
W Sussex
5.26 %
12.63 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
11845
402
3.4%
11946
402
3.4%
Wales
4352
213
4.9%
4374
213
4.9%
Scotland
86
5
5.8%
86
5
5.8%
GB
16283
620
3.8%
xx-xxxx-xxxx
620
3.8%
Table 6a (i): Number of animals and submissions tested in October 2022
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
128217
5022
3.9%
137044
5022
3.7%
Wales
41390
2544
6.1%
44218
2544
5.8%
Scotland
3153
90
2.9%
3316
90
2.7%
GB
172758
7656
4.4%
184578
7656
4.1%
Table 6b (i): Number of animals and submissions tested in 2022 Up to end of October
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
13517
585
4.3%
13688
585
4.3%
Wales
5971
229
3.8%
6102
229
3.8%
GB
19488
814
4.2%
19790
814
4.1%
Table 6a (ii): Number of animals and submissions tested in October 2021
Table 6b (ii): Number of animals and submissions tested in 2021 Up to end of October
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
184880
9855
5.3%
196647
9858
5.0%
Wales
52896
3304
6.2%
56704
3305
5.8%
Scotland
579
136
23.5%
1166
136
11.7%
GB
238345
13295
5.6%
254517
13299
5.2%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples October 2022
Table 7b Number of private samples 2022
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Devon
PRV-SE
4
109
5
4.59 %
PRV-SP
1
2
0
0
Dorset
PRV-SP
2
3
0
0
East Sussex
PRV-SP
1
3
0
0
Gloucestershire
PRV-SP
2
4
0
0
Hampshire
PRV-SE
1
7
2
28.57 %
Herefordshire
PRV-SP
1
1
0
0
Leicestershire and Rutland
PRV-SP
6
6
0
0
Somerset excl North
PRV-SE
1
5
0
0
PRV-SP
3
17
1
5.88 %
Wiltshire
PRV-SE
4
67
9
13.43 %
Total
26
224
17
7.59 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
4
52
19
36.5%
BOV-OTHER
1
18
0
0
Ceredigion
BOV-IR-C
2
10
0
0
North East Wales
BOV-POSTM
1
1
0
0
Pembrokeshire
BOV-IR-C
6
62
11
17.7%
BOV-IR-EX
1
2
0
0
BOV-OTHER
3
173
1
0.6%
Powys
BOV-IR-C
2
13
1
7.7%
BOV-IR-EX
3
20
6
30.0%
South Wales
BOV-IR-C
1
1
1
100.0%
Wales
24
352
39
11.1%
All GB
24
352
39
11.1 %
Table 8a Number of Cattle Antibody Tests October 2022
Table 8b Number of Cattle Antibody Tests 2022
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
Wales
Carmarthenshire
BOV-IR-C
31
286
75
26.2%
BOV-IR-EX
4
22
2
9.1%
BOV-OTHER
5
696
14
2.0%
BOV-P
2
105
2
1.9%
Ceredigion
BOV-IR-C
26
206
34
16.5%
BOV-IR-EX
5
17
3
17.6%
BOV-P
2
62
6
9.7%
BOV-POSTM
1
13
4
30.8%
North East Wales
BOV-IR-C
36
300
30
10.0%
BOV-IR-EX
2
14
4
28.6%
BOV-OTHER
5
209
0
0
BOV-P
3
660
53
8.0%
BOV-POSTM
70
169
33
19.5%
North West Wales
BOV-POSTM
3
3
0
0
Pembrokeshire
BOV-IR-C
132
1,125
235
20.9%
BOV-IR-EX
23
139
39
28.1%
BOV-OTHER
16
1,635
82
5.0%
BOV-P
10
629
33
5.2%
OTHER
1
30
0
0
Powys
BOV-IR-C
22
173
24
13.9%
BOV-IR-EX
3
20
6
30.0%
BOV-P
4
206
5
2.4%
BOV-POSTM
2
2
0
0
Shropshire
BOV-IR-C
1
1
0
0
South Wales
BOV-IR-C
18
154
26
16.9%
BOV-OTHER
3
143
8
5.6%
BOV-P
1
282
16
5.7%
Wales
431
7,301
734
10.1%
All GB
431
7,301
734
10.1 %

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive