Monthly report: Surveillance Project SB4008 IFNg Test for Bovine Tuberculosis - September 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 169
Report Period 1st - 30th September 2020
Issued 16 October 2020

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
September
2020
%
Total 2020
%
Submissions
320
2525
Samples
Total (%)
25394
100.00 %
207049
100.00 %
England
19883
78.30 %
163478
78.96 %
Wales
5483
21.59 %
39041
18.86 %
Scotland
28
0.11 %
4530
2.19 %
Parallel Tests
PAR-CUL-N
10108
39.80 %
75838
36.63 %
PAR-CUL-P
194
0.76 %
7361
3.56 %
PAR-HERD-S
21
0.08 %
1132
0.55 %
PAR-NEW-IN
10420
41.03 %
88907
42.94 %
PAR-OTHER
1111
4.38 %
5177
2.50 %
PAR-PERSIS
3176
12.51 %
23941
11.56 %
PAR-RAPID
99
0.39 %
1172
0.57 %
Total(% of all samples)
25129
98.96 %
203528
98.30 %
Serial/Extended Tests
SER-FLEXI
265
1.04 %
3502
1.69 %
SER-FRAUD
0
0.00 %
19
0.01 %
SER-NSR
0
0.00 %
Total(% of all samples)
265
1.04 %
3521
1.70 %
Total
25394
100.00 %
207049
100.00 %
Retests
Total (% of all samples)
1588
6.25 %
12511
6.04 %
England
1365
5.38 %
9944
4.80 %
Wales
211
0.83 %
2280
1.10 %
Scotland
12
0.05 %
287
0.14 %
Resamples
Total (% of all samples)
1164
4.58 %
9506
4.59 %
England
989
3.89 %
7497
3.62 %
Wales
165
0.65 %
1782
0.86 %
Scotland
10
0.04 %
227
0.11 %
Rejects
Total (% of all samples)
15
0.06 %
2327
1.12 %
England
12
0.05 %
2222
1.07 %
Wales
3
0.01 %
40
0.02 %
Scotland
0
0.00 %
65
0.03 %
Table 1. Monthly Totals for September 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
10108
39.8 %
PAR-CUL-P
194
0.8 %
PAR-HERD-S
21
0.1 %
PAR-NEW-IN
10420
41.0 %
PAR-OTHER
1111
4.4 %
PAR-PERSIS
3176
12.5 %
PAR-RAPID
99
0.4 %
SER-FLEXI
265
1.0 %
Total
25394
100.00 %
Figure 1. Number of samples submitted in each category during September 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
Kirkcudbright
Argyll & Bute
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northants
Lincs
Leics & Rut
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during September 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
Wigtown
Perthshire
Kirkcudbright
Dumfries & G
Berwickshire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
SER-FLEXI
1
7
0.03 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
11
708
2.79 %
12
1.69 %
636
89.83 %
96
13.56 %
60
8.47 %
0
0.00 %
Devon
PAR-CUL-N
26
2,756
10.85 %
69
2.50 %
2562
92.96 %
165
5.99 %
115
4.17 %
10
0.36 %
PAR-CUL-P
2
15
0.06 %
1
6.67 %
13
86.67 %
1
6.67 %
1
6.67 %
0
0.00 %
Dorset
PAR-CUL-N
8
324
1.28 %
8
2.47 %
299
92.28 %
21
6.48 %
17
5.25 %
0
0.00 %
Gloucs
PAR-CUL-N
12
832
3.28 %
26
3.13 %
747
89.78 %
85
10.22 %
58
6.97 %
1
0.12 %
PAR-OTHER
1
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Heref
PAR-NEW-IN
2
28
0.11 %
0
0.00 %
27
96.43 %
3
10.71 %
1
3.57 %
0
0.00 %
PAR-OTHER
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-CUL-N
3
789
3.11 %
16
2.03 %
741
93.92 %
49
6.21 %
32
4.06 %
0
0.00 %
Somerset excl N
PAR-CUL-N
13
2,461
9.69 %
50
2.03 %
2321
94.31 %
115
4.67 %
90
3.66 %
0
0.00 %
PAR-CUL-P
1
156
0.61 %
0
0.00 %
152
97.44 %
5
3.21 %
4
2.56 %
0
0.00 %
SER-FLEXI
4
199
0.78 %
6
3.02 %
189
94.97 %
4
2.01 %
4
2.01 %
0
0.00 %
Staffs
PAR-CUL-N
10
1,653
6.51 %
32
1.94 %
1554
94.01 %
76
4.60 %
66
3.99 %
1
0.06 %
PAR-NEW-IN
4
205
0.81 %
6
2.93 %
190
92.68 %
20
9.76 %
9
4.39 %
0
0.00 %
Wiltshire
PAR-CUL-N
8
585
2.30 %
16
2.74 %
525
89.74 %
60
10.26 %
44
7.52 %
0
0.00 %
PAR-CUL-P
1
23
0.09 %
0
0.00 %
13
56.52 %
12
52.17 %
10
43.48 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
5
740
2.91 %
21
2.84 %
649
87.70 %
92
12.43 %
70
9.46 %
0
0.00 %
PAR-PERSIS
2
315
1.24 %
2
0.63 %
307
97.46 %
8
2.54 %
6
1.90 %
0
0.00 %
Bucks
PAR-NEW-IN
2
237
0.93 %
6
2.53 %
216
91.14 %
23
9.70 %
15
6.33 %
0
0.00 %
Cheshire
PAR-NEW-IN
15
2,885
11.36 %
60
2.08 %
2678
92.82 %
206
7.14 %
147
5.10 %
0
0.00 %
Derbyshire
PAR-NEW-IN
10
1,829
7.20 %
56
3.06 %
1662
90.87 %
147
8.04 %
111
6.07 %
0
0.00 %
E Sussex
PAR-NEW-IN
3
226
0.89 %
5
2.21 %
210
92.92 %
13
5.75 %
11
4.87 %
0
0.00 %
Hampshire
PAR-NEW-IN
7
1,082
4.26 %
32
2.96 %
1022
94.45 %
44
4.07 %
28
2.59 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
1
117
0.46 %
4
3.42 %
107
91.45 %
6
5.13 %
6
5.13 %
0
0.00 %
Table 2a. Summary by County for September 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Oxon
PAR-NEW-IN
1
107
0.42 %
2
1.87 %
105
98.13 %
0
0.00 %
0
0.00 %
0
0.00 %
Warks
PAR-NEW-IN
11
991
3.90 %
35
3.53 %
902
91.02 %
65
6.56 %
54
5.45 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
384
1.51 %
4
1.04 %
363
94.53 %
32
8.33 %
17
4.43 %
0
0.00 %
Cumbria
PAR-NEW-IN
1
4
0.02 %
0
0.00 %
1
25.00 %
3
75.00 %
3
75.00 %
0
0.00 %
PAR-PERSIS
1
33
0.13 %
1
3.03 %
28
84.85 %
4
12.12 %
4
12.12 %
0
0.00 %
Lincs
PAR-NEW-IN
1
81
0.32 %
1
1.23 %
75
92.59 %
5
6.17 %
5
6.17 %
0
0.00 %
N Yorks
PAR-NEW-IN
2
55
0.22 %
1
1.82 %
54
98.18 %
0
0.00 %
0
0.00 %
0
0.00 %
W Yorks
PAR-NEW-IN
2
44
0.17 %
1
2.27 %
43
97.73 %
4
9.09 %
0
0.00 %
0
0.00 %
England
176
19883
78.30 %
473
2.38 %
18409
92.59 %
1365
6.87 %
989
4.97 %
12
0.06 %
Wales - High TB
Carms
PAR-OTHER
4
311
1.22 %
22
7.07 %
278
89.39 %
13
4.18 %
11
3.54 %
0
0.00 %
PAR-PERSIS
6
183
0.72 %
21
11.48 %
157
85.79 %
6
3.28 %
5
2.73 %
0
0.00 %
PAR-RAPID
7
17
0.07 %
8
47.06 %
8
47.06 %
1
5.88 %
1
5.88 %
0
0.00 %
Ceredigion
PAR-OTHER
1
39
0.15 %
10
25.64 %
29
74.36 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
341
1.34 %
12
3.52 %
317
92.96 %
14
4.11 %
12
3.52 %
0
0.00 %
PAR-RAPID
6
7
0.03 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
2
9
0.04 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
8
0.03 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
14
456
1.80 %
59
12.94 %
386
84.65 %
18
3.95 %
11
2.41 %
0
0.00 %
PAR-PERSIS
26
1,687
6.64 %
83
4.92 %
1544
91.52 %
66
3.91 %
58
3.44 %
2
0.12 %
PAR-RAPID
13
45
0.18 %
4
8.89 %
41
91.11 %
1
2.22 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
47
0.19 %
0
0.00 %
47
100.00 %
2
4.26 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
2
20
0.08 %
3
15.00 %
17
85.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
24
0.09 %
4
16.67 %
17
70.83 %
4
16.67 %
3
12.50 %
0
0.00 %
PAR-PERSIS
5
298
1.17 %
9
3.02 %
283
94.97 %
10
3.36 %
6
2.01 %
0
0.00 %
PAR-RAPID
3
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
2
195
0.77 %
13
6.67 %
174
89.23 %
10
5.13 %
8
4.10 %
0
0.00 %
PAR-PERSIS
2
305
1.20 %
1
0.33 %
300
98.36 %
6
1.97 %
4
1.31 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
2
0.01 %
1
50.00 %
0
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
Ceredigion
PAR-RAPID
1
11
0.04 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
8
828
3.26 %
12
1.45 %
797
96.26 %
26
3.14 %
18
2.17 %
1
0.12 %
PAR-OTHER
5
41
0.16 %
3
7.32 %
36
87.80 %
2
4.88 %
2
4.88 %
0
0.00 %
PAR-PERSIS
2
5
0.02 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-RAPID
4
12
0.05 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
4
0.02 %
0
0.00 %
4
100.00 %
2
50.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-NEW-IN
2
28
0.11 %
1
3.57 %
24
85.71 %
3
10.71 %
3
10.71 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
5
546
2.15 %
14
2.56 %
513
93.96 %
23
4.21 %
19
3.48 %
0
0.00 %
PAR-OTHER
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
142
5483
21.59 %
289
5.27 %
5026
91.67 %
211
3.85 %
165
3.01 %
3
0.05 %
Scotland - Scotland
Argyll & Bute
PAR-OTHER
1
27
0.11 %
0
0.00 %
18
66.67 %
11
40.74 %
9
33.33 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Scotland
2
28
0.11 %
0
0.00 %
18
64.29 %
12
42.86 %
10
35.71 %
0
0.00 %
Grand Total
Sum:
320
25394
762
23453
1588
1164
15
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
SER-FLEXI
2
73
0.04 %
1
1.37 %
64
87.67 %
8
10.96 %
8
10.96 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
95
11,014
5.32 %
327
2.97 %
10068
91.41 %
727
6.60 %
519
4.71 %
100
0.91 %
PAR-CUL-P
7
1,802
0.87 %
46
2.55 %
xx-xxxx-xxxx
94.28 %
73
4.05 %
57
3.16 %
0
0.00 %
PAR-PERSIS
17
3,332
1.61 %
135
4.05 %
3079
92.41 %
171
5.13 %
117
3.51 %
1
0.03 %
SER-FLEXI
2
372
0.18 %
9
2.42 %
354
95.16 %
10
2.69 %
9
2.42 %
0
0.00 %
Devon
PAR-CUL-N
228
25,649
12.39 %
1259
4.91 %
23029
89.79 %
1585
6.18 %
1221
4.76 %
140
0.55 %
PAR-CUL-P
12
1,084
0.52 %
60
5.54 %
952
87.82 %
90
8.30 %
70
6.46 %
2
0.18 %
PAR-PERSIS
1
92
0.04 %
8
8.70 %
80
86.96 %
8
8.70 %
4
4.35 %
0
0.00 %
SER-FLEXI
6
312
0.15 %
7
2.24 %
288
92.31 %
14
4.49 %
11
3.53 %
6
1.92 %
Dorset
PAR-CUL-N
50
6,772
3.27 %
370
5.46 %
5983
88.35 %
354
5.23 %
268
3.96 %
151
2.23 %
PAR-CUL-P
10
2,817
1.36 %
257
9.12 %
2443
86.72 %
163
5.79 %
116
4.12 %
1
0.04 %
PAR-NEW-IN
2
167
0.08 %
5
2.99 %
156
93.41 %
11
6.59 %
6
3.59 %
0
0.00 %
SER-FLEXI
8
805
0.39 %
52
6.46 %
734
91.18 %
32
3.98 %
19
2.36 %
0
0.00 %
Gloucs
PAR-CUL-N
49
4,231
2.04 %
176
4.16 %
3746
88.54 %
392
9.26 %
306
7.23 %
3
0.07 %
PAR-NEW-IN
1
38
0.02 %
4
10.53 %
32
84.21 %
4
10.53 %
2
5.26 %
0
0.00 %
PAR-OTHER
2
63
0.03 %
1
1.59 %
55
87.30 %
8
12.70 %
7
11.11 %
0
0.00 %
PAR-PERSIS
2
242
0.12 %
14
5.79 %
220
90.91 %
10
4.13 %
8
3.31 %
0
0.00 %
Heref
PAR-CUL-N
17
2,508
1.21 %
90
3.59 %
2331
92.94 %
117
4.67 %
82
3.27 %
5
0.20 %
PAR-NEW-IN
9
654
0.32 %
23
3.52 %
586
89.60 %
59
9.02 %
44
6.73 %
1
0.15 %
PAR-OTHER
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
213
0.10 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Shropshire
PAR-CUL-N
13
3,158
1.53 %
61
1.93 %
2984
94.49 %
164
5.19 %
111
3.51 %
2
0.06 %
PAR-PERSIS
7
1,015
0.49 %
60
5.91 %
899
88.57 %
65
6.40 %
54
5.32 %
2
0.20 %
SER-FLEXI
3
389
0.19 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
96
10,806
5.22 %
410
3.79 %
10019
92.72 %
519
4.80 %
348
3.22 %
29
0.27 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
2
417
0.20 %
13
3.12 %
390
93.53 %
18
4.32 %
14
3.36 %
0
0.00 %
PAR-PERSIS
3
293
0.14 %
25
8.53 %
258
88.05 %
14
4.78 %
10
3.41 %
0
0.00 %
SER-FLEXI
4
199
0.10 %
6
3.02 %
189
94.97 %
4
2.01 %
4
2.01 %
0
0.00 %
Staffs
PAR-CUL-N
18
2,755
1.33 %
73
2.65 %
2406
87.33 %
137
4.97 %
120
4.36 %
156
5.66 %
PAR-NEW-IN
50
6,438
3.11 %
311
4.83 %
5897
91.60 %
303
4.71 %
229
3.56 %
1
0.02 %
Wiltshire
PAR-CUL-N
62
7,714
3.73 %
202
2.62 %
7091
91.92 %
535
6.94 %
401
5.20 %
20
0.26 %
PAR-CUL-P
7
1,241
0.60 %
69
5.56 %
1096
88.32 %
95
7.66 %
65
5.24 %
11
0.89 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
104
0.05 %
5
4.81 %
93
89.42 %
7
6.73 %
6
5.77 %
0
0.00 %
SER-FLEXI
2
45
0.02 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
24
2,641
1.28 %
75
2.84 %
2369
89.70 %
255
9.66 %
197
7.46 %
0
0.00 %
PAR-PERSIS
2
315
0.15 %
2
0.63 %
307
97.46 %
8
2.54 %
6
1.90 %
0
0.00 %
Bucks
PAR-NEW-IN
14
1,694
0.82 %
68
4.01 %
1513
89.32 %
143
8.44 %
113
6.67 %
0
0.00 %
Cheshire
PAR-CUL-N
6
868
0.42 %
14
1.61 %
837
96.43 %
28
3.23 %
17
1.96 %
0
0.00 %
PAR-NEW-IN
125
20,577
9.94 %
440
2.14 %
19224
93.42 %
1117
5.43 %
863
4.19 %
50
0.24 %
Derbyshire
PAR-NEW-IN
113
13,256
6.40 %
412
3.11 %
11348
85.61 %
932
7.03 %
744
5.61 %
752
5.67 %
E Sussex
PAR-NEW-IN
25
1,894
0.91 %
63
3.33 %
1747
92.24 %
97
5.12 %
84
4.44 %
0
0.00 %
Hampshire
PAR-CUL-N
2
363
0.18 %
15
4.13 %
327
90.08 %
39
10.74 %
19
5.23 %
2
0.55 %
PAR-NEW-IN
31
3,577
1.73 %
83
2.32 %
3320
92.82 %
255
7.13 %
174
4.86 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
40
3,811
1.84 %
157
4.12 %
3465
90.92 %
242
6.35 %
171
4.49 %
18
0.47 %
Northants
PAR-NEW-IN
18
1,131
0.55 %
52
4.60 %
1022
90.36 %
66
5.84 %
56
4.95 %
1
0.09 %
Notts
PAR-NEW-IN
6
366
0.18 %
4
1.09 %
350
95.63 %
17
4.64 %
11
3.01 %
1
0.27 %
Oxon
PAR-NEW-IN
80
7,485
3.62 %
339
4.53 %
6515
87.04 %
470
6.28 %
343
4.58 %
288
3.85 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Warks
PAR-NEW-IN
55
4,705
2.27 %
132
2.81 %
4253
90.39 %
281
5.97 %
216
4.59 %
104
2.21 %
PAR-PERSIS
2
479
0.23 %
20
4.18 %
446
93.11 %
20
4.18 %
13
2.71 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
384
0.19 %
4
1.04 %
363
94.53 %
32
8.33 %
17
4.43 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Cleveland
PAR-NEW-IN
1
50
0.02 %
2
4.00 %
48
96.00 %
1
2.00 %
0
0.00 %
0
0.00 %
Cumbria
PAR-NEW-IN
2
34
0.02 %
0
0.00 %
30
88.24 %
5
14.71 %
4
11.76 %
0
0.00 %
PAR-PERSIS
1
33
0.02 %
1
3.03 %
28
84.85 %
4
12.12 %
4
12.12 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
7
336
0.16 %
6
1.79 %
114
33.93 %
6
1.79 %
6
1.79 %
210
62.50 %
Humberside
PAR-NEW-IN
2
78
0.04 %
7
8.97 %
66
84.62 %
7
8.97 %
5
6.41 %
0
0.00 %
Lincs
PAR-NEW-IN
6
331
0.16 %
15
4.53 %
300
90.63 %
17
5.14 %
16
4.83 %
0
0.00 %
PAR-PERSIS
2
34
0.02 %
0
0.00 %
31
91.18 %
5
14.71 %
3
8.82 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
49
0.02 %
1
2.04 %
29
59.18 %
6
12.24 %
6
12.24 %
13
26.53 %
Northumb
PAR-NEW-IN
2
307
0.15 %
17
5.54 %
240
78.18 %
40
13.03 %
50
16.29 %
0
0.00 %
N Yorks
PAR-NEW-IN
10
849
0.41 %
16
1.88 %
713
83.98 %
40
4.71 %
29
3.42 %
91
10.72 %
Surrey
PAR-NEW-IN
3
429
0.21 %
17
3.96 %
396
92.31 %
18
4.20 %
16
3.73 %
0
0.00 %
W Yorks
PAR-NEW-IN
8
563
0.27 %
4
0.71 %
473
84.01 %
37
6.57 %
25
4.44 %
61
10.83 %
England
1390
163478
78.96 %
6074
3.72 %
147685
90.34 %
9944
6.08 %
7497
4.59 %
2222
1.36 %
Wales - High TB
Carms
PAR-HERD-S
12
678
0.33 %
51
7.52 %
598
88.20 %
45
6.64 %
29
4.28 %
0
0.00 %
PAR-OTHER
38
776
0.37 %
101
13.02 %
640
82.47 %
42
5.41 %
35
4.51 %
0
0.00 %
PAR-PERSIS
51
3,023
1.46 %
227
7.51 %
2666
88.19 %
162
5.36 %
125
4.13 %
5
0.17 %
PAR-RAPID
66
247
0.12 %
45
18.22 %
186
75.30 %
24
9.72 %
16
6.48 %
0
0.00 %
SER-FLEXI
6
84
0.04 %
22
26.19 %
59
70.24 %
2
2.38 %
1
1.19 %
2
2.38 %
Ceredigion
PAR-HERD-S
2
13
0.01 %
0
0.00 %
7
53.85 %
6
46.15 %
6
46.15 %
0
0.00 %
PAR-OTHER
28
363
0.18 %
32
8.82 %
315
86.78 %
23
6.34 %
16
4.41 %
0
0.00 %
PAR-PERSIS
27
1,207
0.58 %
56
4.64 %
1097
90.89 %
61
5.05 %
52
4.31 %
2
0.17 %
PAR-RAPID
33
101
0.05 %
28
27.72 %
68
67.33 %
7
6.93 %
5
4.95 %
0
0.00 %
SER-FLEXI
2
40
0.02 %
0
0.00 %
38
95.00 %
2
5.00 %
2
5.00 %
0
0.00 %
Gwent
PAR-HERD-S
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
18
1,091
0.53 %
61
5.59 %
1001
91.75 %
47
4.31 %
28
2.57 %
1
0.09 %
PAR-RAPID
17
109
0.05 %
18
16.51 %
88
80.73 %
3
2.75 %
3
2.75 %
0
0.00 %
SER-FLEXI
8
256
0.12 %
24
9.38 %
226
88.28 %
10
3.91 %
6
2.34 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Pembs
PAR-HERD-S
7
292
0.14 %
11
3.77 %
271
92.81 %
12
4.11 %
10
3.42 %
0
0.00 %
PAR-OTHER
96
1,667
0.81 %
213
12.78 %
1397
83.80 %
80
4.80 %
57
3.42 %
0
0.00 %
PAR-PERSIS
127
7,767
3.75 %
370
4.76 %
7052
90.79 %
413
5.32 %
335
4.31 %
10
0.13 %
PAR-RAPID
99
427
0.21 %
89
20.84 %
326
76.35 %
17
3.98 %
12
2.81 %
0
0.00 %
SER-FLEXI
20
424
0.20 %
15
3.54 %
396
93.40 %
22
5.19 %
13
3.07 %
0
0.00 %
Powys
PAR-HERD-S
12
72
0.03 %
9
12.50 %
63
87.50 %
2
2.78 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
113
0.05 %
6
5.31 %
106
93.81 %
1
0.88 %
1
0.88 %
0
0.00 %
PAR-OTHER
14
800
0.39 %
46
5.75 %
676
84.50 %
87
10.88 %
78
9.75 %
0
0.00 %
PAR-PERSIS
52
1,778
0.86 %
103
5.79 %
1516
85.26 %
175
9.84 %
150
8.44 %
9
0.51 %
PAR-RAPID
39
123
0.06 %
20
16.26 %
100
81.30 %
4
3.25 %
3
2.44 %
0
0.00 %
SER-FLEXI
10
212
0.10 %
17
8.02 %
186
87.74 %
19
8.96 %
9
4.25 %
0
0.00 %
S Wales
PAR-PERSIS
2
274
0.13 %
12
4.38 %
260
94.89 %
8
2.92 %
2
0.73 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
36
0.02 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-PERSIS
3
16
0.01 %
0
0.00 %
14
87.50 %
3
18.75 %
2
12.50 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
30
0.01 %
5
16.67 %
24
80.00 %
3
10.00 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
1
155
0.07 %
34
21.94 %
119
76.77 %
2
1.29 %
2
1.29 %
0
0.00 %
PAR-OTHER
9
224
0.11 %
18
8.04 %
197
87.95 %
11
4.91 %
9
4.02 %
0
0.00 %
PAR-PERSIS
8
567
0.27 %
32
5.64 %
519
91.53 %
19
3.35 %
16
2.82 %
0
0.00 %
PAR-RAPID
7
11
0.01 %
6
54.55 %
4
36.36 %
1
9.09 %
1
9.09 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
171
0.08 %
12
7.02 %
152
88.89 %
8
4.68 %
7
4.09 %
0
0.00 %
PAR-RAPID
5
29
0.01 %
8
27.59 %
21
72.41 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
48
0.02 %
6
12.50 %
40
83.33 %
2
4.17 %
2
4.17 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
82
7,949
3.84 %
215
2.70 %
7344
92.39 %
474
5.96 %
388
4.88 %
2
0.03 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
25
368
0.18 %
29
7.88 %
320
86.96 %
23
6.25 %
19
5.16 %
0
0.00 %
PAR-PERSIS
18
1,552
0.75 %
90
5.80 %
1410
90.85 %
63
4.06 %
49
3.16 %
3
0.19 %
PAR-RAPID
23
72
0.03 %
18
25.00 %
51
70.83 %
3
4.17 %
3
4.17 %
0
0.00 %
SER-FLEXI
9
185
0.09 %
7
3.78 %
169
91.35 %
15
8.11 %
7
3.78 %
2
1.08 %
Powys
PAR-NEW-IN
13
914
0.44 %
18
1.97 %
849
92.89 %
57
6.24 %
46
5.03 %
1
0.11 %
PAR-OTHER
4
103
0.05 %
1
0.97 %
89
86.41 %
18
17.48 %
13
12.62 %
0
0.00 %
PAR-PERSIS
3
280
0.14 %
4
1.43 %
260
92.86 %
18
6.43 %
16
5.71 %
0
0.00 %
PAR-RAPID
3
4
0.00 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
13
0.01 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-RAPID
1
7
0.00 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
2
44
0.02 %
0
0.00 %
42
95.45 %
2
4.55 %
2
4.55 %
0
0.00 %
PAR-NEW-IN
28
1,695
0.82 %
31
1.83 %
1591
93.86 %
97
5.72 %
70
4.13 %
3
0.18 %
PAR-OTHER
3
9
0.00 %
0
0.00 %
5
55.56 %
4
44.44 %
4
44.44 %
0
0.00 %
PAR-RAPID
2
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
20
2,510
1.21 %
42
1.67 %
2353
93.75 %
165
6.57 %
115
4.58 %
0
0.00 %
PAR-OTHER
3
14
0.01 %
1
7.14 %
11
78.57 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-PERSIS
1
40
0.02 %
0
0.00 %
32
80.00 %
10
25.00 %
8
20.00 %
0
0.00 %
PAR-RAPID
3
20
0.01 %
1
5.00 %
19
95.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
1086
39041
18.86 %
2168
5.55 %
35051
89.78 %
2280
5.84 %
1782
4.56 %
40
0.10 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
7
588
0.28 %
32
5.44 %
455
77.38 %
41
6.97 %
36
6.12 %
65
11.05 %
PAR-OTHER
7
782
0.38 %
15
1.92 %
700
89.51 %
94
12.02 %
67
8.57 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
383
0.18 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Berwickshire
PAR-NEW-IN
2
78
0.04 %
8
10.26 %
62
79.49 %
8
10.26 %
8
10.26 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
6
849
0.41 %
12
1.41 %
802
94.46 %
38
4.48 %
35
4.12 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
15
807
0.39 %
22
2.73 %
762
94.42 %
34
4.21 %
23
2.85 %
0
0.00 %
PAR-PERSIS
1
22
0.01 %
0
0.00 %
22
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Perthshire
PAR-NEW-IN
2
357
0.17 %
0
0.00 %
345
96.64 %
14
3.92 %
12
3.36 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wigtown
PAR-NEW-IN
6
664
0.32 %
17
2.56 %
624
93.98 %
28
4.22 %
23
3.46 %
0
0.00 %
Scotland
49
4530
2.19 %
107
2.36 %
4131
91.19 %
287
6.34 %
227
5.01 %
65
1.43 %
Grand Total
Sum:
xx-xxxx-xxxx
8349
186867
12511
9506
2327
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol September 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
171
19677
99.0%
467
2.4%
18214
92.6%
1360
6.9%
984
5.0%
12
0.1%
Serial/Extended
5
206
1.0%
6
2.9%
195
94.7%
5
2.4%
5
2.4%
0
0.0 %
Total
176
19883
100.0%
473
2.4%
18409
92.6%
1365
6.9%
989
5.0%
12
0.1%
Scotland
Parallel
2
28
100.0%
0
0.0 %
18
64.3%
12
42.9%
10
35.7%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
28
100.0%
0
0.0 %
18
64.3%
12
42.9%
10
35.7%
0
0.0 %
Wales
Parallel
134
5424
98.9%
287
5.3%
4969
91.6%
207
3.8%
165
3.0%
3
0.1%
Serial/Extended
8
59
1.1%
2
3.4%
57
96.6%
4
6.8%
0
0.0 %
0
0.0 %
Total
142
5483
100.0%
289
5.3%
5026
91.7%
211
3.8%
165
3.0%
3
0.1%
GB
Parallel
307
25129
99.0%
754
3.0%
23201
92.3%
1579
6.3%
1159
4.6%
15
0.1%
Serial/Extended
13
265
1.0%
8
3.0%
252
95.1%
9
3.4%
5
1.9%
0
0.0 %
Total
320
25394
100.0%
762
3.0%
23453
92.4%
1588
6.3%
1164
4.6%
15
0.1%
176.00
142.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
1361 161264
98.6%
5979
3.7% 145644
90.3%
9849
6.1%
7425
4.6%
2216
1.4%
Serial/Extended
29
2214
1.4%
95
4.3%
2041
92.2%
95
4.3%
72
3.3%
6
0.3%
Total
1390 163478
100.0%
6074
3.7% 147685
90.3%
9944
6.1%
7497
4.6%
2222
1.4%
Scotland
Parallel
49
4530
100.0%
107
2.4%
4131
91.2%
287
6.3%
227
5.0%
65
1.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
49
4530
100.0%
107
2.4%
4131
91.2%
287
6.3%
227
5.0%
65
1.4%
Wales
Parallel
1024
37734
96.7%
2071
5.5%
33885
89.8%
2208
5.9%
1742
4.6%
36
0.1%
Serial/Extended
62
1307
3.3%
97
7.4%
1166
89.2%
72
5.5%
40
3.1%
4
0.3%
Total
1086
39041
100.0%
2168
5.6%
35051
89.8%
2280
5.8%
1782
4.6%
40
0.1%
GB
Parallel
xx-xxxx-xxxx
98.3%
8157
4.0% 183660
90.2%
12344
6.1%
9394
4.6%
2317
1.1%
Serial/Extended
91
3521
1.7%
192
5.5%
3207
91.1%
167
4.7%
112
3.2%
10
0.3%
Total
xx-xxxx-xxxx
100.0%
8349
4.0% 186867
90.3%
12511
6.0%
9506
4.6%
2327
1.1%
#######
#######
49.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
265
1.0%
8
3.0%
252
95.1%
9
3.4 %
5
1.9 %
0
Parallel - Herd or Group Slaughter
3
21
0.1%
3
14.3%
17
81.0%
1
4.8 %
1
4.8 %
0
Parallel New Infection
88
10420
41.0%
261
2.5%
9640
92.5%
716
6.9 %
518
5.0 %
1
0.0 %
Parallel - new infection cull area
91
10108
39.8%
229
2.3%
9385
92.8%
667
6.6 %
482
4.8 %
12
0.1 %
Parallel - Other
33
1111
4.4%
111
10.0%
956
86.0%
58
5.2 %
44
4.0 %
0
Parallel Persistent Infection
52
3176
12.5%
129
4.1%
2948
92.8%
116
3.7 %
97
3.1 %
2
0.1 %
Parallel - persistent infection cull area
4
194
0.8%
1
0.5%
178
91.8%
18
9.3 %
15
7.7 %
0
Parallel - Rapid Testing Two-times IRs
36
99
0.4%
20
20.2%
77
77.8%
3
3.0 %
2
2.0 %
0
Total
320
25394
100.0%
762
3.0%
23453
92.4%
1588
6.3 %
1164
4.6 %
15
0.1 %
Table 4a. Summaryof IFN Gamma results by submission reason (September 2020)
England - HRA
Extended Flexible IFN-GAMMA test
5
206
0.8%
6
2.9%
195
94.7%
5
2.4 %
5
2.4 %
0
Parallel New Infection
6
233
0.9%
6
2.6%
217
93.1%
23
9.9 %
10
4.3 %
0
Parallel - new infection cull area
91
10108
39.8%
229
2.3%
9385
92.8%
667
6.6 %
482
4.8 %
12
0.1 %
Parallel - Other
2
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Parallel - persistent infection cull area
4
194
0.8%
1
0.5%
178
91.8%
18
9.3 %
15
7.7 %
0
England - HRA
108
10752
42.3%
242
2.3%
9986
92.9%
713
6.6 %
512
4.8 %
12
0.1 %
England - Edge
Parallel New Infection
56
8215
32.4%
221
2.7%
7552
91.9%
596
7.3 %
442
5.4 %
0
Parallel Persistent Infection
2
315
1.2%
2
0.6%
307
97.5%
8
2.5 %
6
1.9 %
0
England - Edge
58
8530
33.6%
223
2.6%
7859
92.1%
604
7.1 %
448
5.3 %
0
England - LRA
Parallel New Infection
9
568
2.2%
7
1.2%
536
94.4%
44
7.7 %
25
4.4 %
0
Parallel Persistent Infection
1
33
0.1%
1
3.0%
28
84.8%
4
12.1 %
4
12.1 %
0
England - LRA
10
601
2.4%
8
1.3%
564
93.8%
48
8.0 %
29
4.8 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
7
55
0.2%
2
3.6%
53
96.4%
2
3.6 %
0
0
Parallel - Herd or Group Slaughter
2
20
0.1%
3
15.0%
17
85.0%
0
0
0
Parallel - Other
21
830
3.3%
95
11.4%
710
85.5%
35
4.2 %
25
3.0 %
0
Parallel Persistent Infection
45
2518
9.9%
125
5.0%
2309
91.7%
97
3.9 %
82
3.3 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
30
74
0.3%
14
18.9%
59
79.7%
2
2.7 %
1
1.4 %
0
Wales - High TB
105
3497
13.8%
239
6.8%
3148
90.0%
136
3.9 %
108
3.1 %
2
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
4
0.0%
0
0.0 %
4
100.0%
2
50.0 %
0
0
Parallel New Infection
9
829
3.3%
12
1.4%
798
96.3%
26
3.1 %
18
2.2 %
1
0.1 %
Parallel - Other
7
236
0.9%
16
6.8%
210
89.0%
12
5.1 %
10
4.2 %
0
Parallel Persistent Infection
4
310
1.2%
1
0.3%
304
98.1%
7
2.3 %
5
1.6 %
0
Parallel - Rapid Testing Two-times IRs
6
25
0.1%
6
24.0%
18
72.0%
1
4.0 %
1
4.0 %
0
Wales - Intermediate TB
27
1404
5.5%
35
2.5%
1334
95.0%
48
3.4 %
34
2.4 %
1
0.1 %
Wales - Low TB
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel New Infection
7
574
2.3%
15
2.6%
537
93.6%
26
4.5 %
22
3.8 %
0
Parallel - Other
2
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Wales - Low TB
10
582
2.3%
15
2.6%
544
93.5%
27
4.6 %
23
4.0 %
0
Scotland - Scotland
Parallel New Infection
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel - Other
1
27
0.1%
0
0.0 %
18
66.7%
11
40.7 %
9
33.3 %
0
Scotland - Scotland
2
28
0.1%
0
0.0 %
18
64.3%
12
42.9 %
10
35.7 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
89
3502
1.7%
192
5.5%
3188
91.0%
167
4.8 %
112
3.2 %
10
0.3 %
Parallel - Herd or Group Slaughter
39
1132
0.5%
77
6.8%
1007
89.0%
70
6.2 %
48
4.2 %
0
Parallel New Infection
828
88907
42.9%
2695
3.0%
80341
90.4%
5450
6.1 %
4209
4.7 %
1662
1.9 %
Parallel - new infection cull area
636
75838
36.6%
2997
4.0%
68821
90.7%
4597
6.1 %
3412
4.5 %
608
0.8 %
Parallel - Other
232
5177
2.5%
458
8.8%
4409
85.2%
395
7.6 %
310
6.0 %
0
Parallel Persistent Infection
357
23941
11.6%
1246
5.2%
21615
90.3%
1331
5.6 %
1047
4.4 %
33
0.1 %
Parallel - persistent infection cull area
38
7361
3.6%
445
6.0%
6580
89.4%
439
6.0 %
322
4.4 %
14
0.2 %
Parallel - Rapid Testing Two-times IRs
304
1172
0.6%
239
20.4%
887
75.7%
62
5.3 %
46
3.9 %
0
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
8349
4.0%
186867
90.3%
12511
6.0 %
9506
4.6 %
2327
1.1 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
27
2195
1.1%
95
4.3%
2022
92.1%
95
4.3 %
72
3.3 %
6
0.3 %
Parallel New Infection
63
7298
3.5%
343
4.7%
6672
91.4%
377
5.2 %
281
3.9 %
2
0.0 %
Parallel - new infection cull area
628
74607
36.0%
2968
4.0%
67657
90.7%
4530
6.1 %
3376
4.5 %
606
0.8 %
Parallel - Other
3
67
0.0%
1
1.5%
59
88.1%
8
11.9 %
7
10.4 %
0
Parallel Persistent Infection
34
5291
2.6%
256
4.8%
4801
90.7%
307
5.8 %
231
4.4 %
3
0.1 %
Parallel - persistent infection cull area
38
7361
3.6%
445
6.0%
6580
89.4%
439
6.0 %
322
4.4 %
14
0.2 %
Serial - Anomalous Reaction
2
19
0.0%
0
0.0 %
19
100.0%
0
0
0
England - Edge
Parallel New Infection
531
61137
29.5%
1825
3.0%
55126
90.2%
3875
6.3 %
2972
4.9 %
1214
2.0 %
Parallel - new infection cull area
8
1231
0.6%
29
2.4%
1164
94.6%
67
5.4 %
36
2.9 %
2
0.2 %
Parallel Persistent Infection
5
795
0.4%
22
2.8%
754
94.8%
28
3.5 %
19
2.4 %
0
England - LRA
Parallel New Infection
48
3410
1.6%
89
2.6%
2772
81.3%
209
6.1 %
174
5.1 %
375
11.0 %
Parallel Persistent Infection
3
67
0.0%
1
1.5%
59
88.1%
9
13.4 %
7
10.4 %
0
England
1390 163478
79.0%
6074
3.7%
147685
90.3%
9944
6.1 %
7497
4.6 %
2222
1.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
48
1061
0.5%
79
7.4%
949
89.4%
55
5.2 %
31
2.9 %
2
0.2 %
Parallel - Herd or Group Slaughter
34
1058
0.5%
72
6.8%
941
88.9%
65
6.1 %
45
4.3 %
0
Parallel New Infection
1
113
0.1%
6
5.3%
106
93.8%
1
0.9 %
1
0.9 %
0

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2020) Cont.
Parallel - Other
178
3610
1.7%
393
10.9%
3028
83.9%
235
6.5 %
189
5.2 %
0
Parallel Persistent Infection
280
15156
7.3%
829
5.5%
13606
89.8%
869
5.7 %
694
4.6 %
27
0.2 %
Parallel - Rapid Testing Two-times IRs
258
1015
0.5%
203
20.0%
773
76.2%
55
5.4 %
39
3.8 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
14
246
0.1%
18
7.3%
217
88.2%
17
6.9 %
9
3.7 %
2
0.8 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
96
9018
4.4%
267
3.0%
8312
92.2%
533
5.9 %
436
4.8 %
3
0.0 %
Parallel - Other
38
695
0.3%
48
6.9%
606
87.2%
52
7.5 %
41
5.9 %
0
Parallel Persistent Infection
33
2570
1.2%
138
5.4%
2341
91.1%
108
4.2 %
88
3.4 %
3
0.1 %
Parallel - Rapid Testing Two-times IRs
41
125
0.1%
35
28.0%
86
68.8%
4
3.2 %
4
3.2 %
0
Wales - Low TB
Parallel - Herd or Group Slaughter
2
44
0.0%
0
0.0 %
42
95.5%
2
4.5 %
2
4.5 %
0
Parallel New Infection
48
4205
2.0%
73
1.7%
3944
93.8%
262
6.2 %
185
4.4 %
3
0.1 %
Parallel - Other
6
23
0.0%
1
4.3%
16
69.6%
6
26.1 %
6
26.1 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
5
32
0.0%
1
3.1%
28
87.5%
3
9.4 %
3
9.4 %
0
Wales
1086
39041
18.9%
2168
5.6%
35051
89.8%
2280
5.8 %
1782
4.6 %
40
0.1 %
Scotland - Scotland
Parallel New Infection
41
3726
1.8%
92
2.5%
3409
91.5%
193
5.2 %
160
4.3 %
65
1.7 %
Parallel - Other
7
782
0.4%
15
1.9%
700
89.5%
94
12.0 %
67
8.6 %
0
Parallel Persistent Infection
1
22
0.0%
0
0.0 %
22
100.0%
0
0
0
Scotland
49
4530
2.2%
107
2.4%
4131
91.2%
287
6.3 %
227
5.0 %
65
1.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2019 October
298
26690
154
743
0
0
0
0
0
58
1
0
5
0
604
0
71
0
3
November
272
30186
169
1080
14
0
0
0
0
72
5
0
15
0
910
0
61
0
1
December
234
25192
138
904
2
0
0
0
0
0
87
2
0
4
0
759
0
49
0
1
2020 January
343
31274
227
1367
1
7
0
0
0
0
178
3
0
8
0
1110
1
57
0
0
February
323
33146
192
1289
1
21
0
0
0
0
187
3
0
6
0
982
0
89
0
0
March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
54
0
1
July
332
22622
180
1153
28
0
0
0
0
203
0
0
8
0
843
4
64
0
3
August
290
24676
173
888
27
0
0
0
0
145
0
4
0
0
634
75
0
0
3
September
320
25394
175
762
126
161
0
0
0
0
30
0
1
0
0
260
19
0
0
0
Totals (last
12 months)
3329
289117
1927
11076
134
284
0
0
0
0
1232
20
5
61
0
8477
100
579
0
13
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1866
226458
1156
7951
208
0
0
0
0
1119
10
4
27
0
6359
20
39
0
3
Scotland
55
5006
25
114
1
0
0
0
0
13
0
0
5
0
78
0
17
0
0
Wales
1408
57653
746
3011
75
0
0
0
0
100
10
1
29
0
2040
80
523
0
10
Totals
3329
289117
1927
11076
284
0
0
0
0
1232
20
5
61
0
8477
100
579
0
13
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2018
2019
2020
IFNg Positive herds
IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2018
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2018
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
50
100
150
200
250
W Yorks
Warks
Somerset excl N
Powys
Oxon
Northants
Lincs
Kirkcudbright
Hampshire
Gwent
E Sussex
Devon
Cumbria
Cheshire
Carms
Berks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during September 2020 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
33.33 %
40.74 %
Avon
14.29 %
14.29 %
Beds
4.43 %
8.33 %
Berks
7.20 %
9.48 %
Bucks
6.33 %
9.70 %
Carms
2.96 %
3.65 %
Ceredigion
3.02 %
3.52 %
Cheshire
5.10 %
7.14 %
Corn & Scilly
8.47 %
13.56 %
Cumbria
18.92 %
18.92 %
Derbyshire
6.07 %
8.04 %
Devon
4.19 %
5.99 %
Dorset
5.25 %
6.48 %
E Sussex
4.87 %
5.75 %
Gloucs
6.91 %
10.13 %
Gwent
5.56 %
5.56 %
Gwynedd
12.90 %
12.90 %
Hampshire
2.59 %
4.07 %
Heref
3.13 %
9.38 %
Kirkcudbright
100.00 %
100.00 %
Leics & Rut
Lincs
6.17 %
6.17 %
NE Wales
2.78 %
3.75 %
Northants
5.13 %
5.13 %
N Yorks
Oxon
Pembs
3.09 %
3.89 %
Powys
2.59 %
4.03 %
Shropshire
4.06 %
6.21 %
Somerset excl
3.48 %
4.40 %
Staffs
4.04 %
5.17 %
Warks
5.45 %
6.56 %
Wiltshire
8.88 %
11.84 %
W Yorks
9.09 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19656
473
2.4%
19883
473
2.4%
Wales
5429
289
5.3%
5483
289
5.3%
Scotland
28
0
0.0 %
28
0.0 %
GB
25113
762
3.0%
25394
762
3.0%
Table 6a (i): Number of animals and submissions tested in September 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
155173
6074
3.9%
163478
6074
3.7%
Wales
36230
2167
6.0%
39041
2168
5.6%
Scotland
3635
107
2.9%
4530
107
2.4%
GB
195037
8348
4.3%
207049
8349
4.0%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of September
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20426
563
2.8%
20801
563
2.7%
Wales
4458
130
2.9%
4509
130
2.9%
Scotland
7
0
0.0 %
7
0.0 %
GB
24891
693
2.8%
25317
693
2.7%
Table 6a (ii): Number of animals and submissions tested in September 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of September
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
181696
8149
4.5%
200794
8149
4.1%
Wales
42681
2355
5.5%
46887
2355
5.0%
Scotland
1889
83
4.4%
2217
83
3.7%
GB
226262
10587
4.7%
249898
10587
4.2%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Dorset
PRV-SP
1
1
0
0
East Sussex
PRV-SP
1
1
0
0
Somerset excl North
PRV-SE
1
28
0
0
PRV-SP
1
1
0
0
Total
4
31
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples September 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SP
1
1
0
0
Cumbria
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
2
4
0
0
East Sussex
PRV-SP
1
1
0
0
Essex
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Hampshire
PRV-SP
1
3
0
0
Oxfordshire
PRV-SP
1
32
0
0
Somerset excl North
PRV-SE
1
28
0
0
PRV-SP
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
17
235
3
1.28 %
Isle of Man
SER-IOM
15
173
0
0
Isle of Man
SER-IOM
3
38
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Berkshire
1
52
4
7.7%
Carmarthenshire
1
19
4
21.1%
Ceredigion
5
134
29
21.6%
North East Wales
4
205
14
6.8%
Pembrokeshire
13
233
38
16.3%
Powys
7
89
10
11.2%
South Wales
4
10
2
20.0%
TC0077
35
742
101
13.6 %
Table 8a Number of Cattle Antibody Tests September 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Berkshire
1
52
4
7.7%
Carmarthenshire
39
619
113
18.3%
Ceredigion
28
356
60
16.9%
Devon
4
1,032
95
9.2%
North East Wales
36
934
96
10.3%
Pembrokeshire
99
1,585
236
14.9%
Powys
31
430
69
16.0%
South Wales
21
338
62
18.3%
Staffordshire
1
22
1
4.5%
Wiltshire
1
291
3
1.0%
TC0077
261
5,659
739
13.1 %
Table 8b Number of Cattle Antibody Tests 2020