Monthly report: Survellience Project SB4008 IGNg tests for Bovine tuberculosis - December 2018
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 148
Report Period 1st - 31st December 2018
Issued 22 January 2019

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
December
2018
%
Total 2018
%
Submissions
228
2669
Samples
Total (%)
21554
100.00 %
253486
100.00 %
England
15274
70.86 %
193638
76.39 %
Wales
6280
29.14 %
57607
22.73 %
Scotland
2241
0.88 %
Parallel Tests
PAR-CUL-N
2693
12.49 %
31400
12.39 %
PAR-CUL-P
424
1.97 %
6710
2.65 %
PAR-HERD-S
1631
7.57 %
8132
3.21 %
PAR-NEW-IN
11486
53.29 %
131838
52.01 %
PAR-OTHER
1128
5.23 %
5134
2.03 %
PAR-PERSIS
3814
17.70 %
59225
23.36 %
PAR-RAPID
63
0.29 %
1570
0.62 %
Total(% of all samples)
21239
98.54 %
244009
96.26 %
Serial/Extended Tests
SER-FLEXI
315
1.46 %
9178
3.62 %
SER-FRAUD
0
0.00 %
270
0.11 %
SER-NSR
0
0.00 %
29
0.01 %
SER-OTHER
0
0.00 %
Total(% of all samples)
315
1.46 %
9477
3.74 %
Total
21554
100.00 %
253486
100.00 %
Retests
Total (% of all samples)
xx-xxxx-xxxx
7.62 %
20862
8.23 %
England
1192
5.53 %
15940
6.29 %
Wales
450
2.09 %
4721
1.86 %
Scotland
201
0.08 %
Resamples
Total (% of all samples)
1211
5.62 %
14410
5.68 %
England
918
4.26 %
10905
4.30 %
Wales
293
1.36 %
3366
1.33 %
Scotland
139
0.05 %
Rejects
Total (% of all samples)
131
0.61 %
2374
0.94 %
England
110
0.51 %
1146
0.45 %
Wales
21
0.10 %
1228
0.48 %
Scotland
0
0.00 %
Table 1. Monthly Totals for December 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
2693
12.5 %
PAR-CUL-P
424
2.0 %
PAR-HERD-S
1631
7.6 %
PAR-NEW-IN
11486
53.3 %
PAR-OTHER
1128
5.2 %
PAR-PERSIS
3814
17.7 %
PAR-RAPID
63
0.3 %
SER-FLEXI
315
1.5 %
Total
21554
100.00 %
Figure 1. Number of samples submitted in each category during December 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
W Glamorgan
S Wales
Shropshire
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Warks
Somerset
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Isle of wight
Heref
Hampshire
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during December 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
Wigtown
West lothian
Perthshire
Ayrshire
Argyll & Bute
Aberdeenshire
W Glamorgan
S Wales
Shropshire
Powys
Pembs
Pembrokshire
NE Wales
Mid glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Sussex
W Midlands
Wiltshire
Warks
S Yorks
Staffs
Somerset
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Merseyside
Lincs
Leics & Rut
Kent
Isle of wight
Humberside
Herts
Heref
Hampshire
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Cambs
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2018
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Table 2a. Summary by County for December 2018
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Corn & Scilly
PAR-CUL-N
7
291
1.35 %
5
1.72 %
264
90.72 %
33
11.34 %
22
7.56 %
0
0.00 %
PAR-NEW-IN
1
183
0.85 %
6
3.28 %
154
84.15 %
28
15.30 %
23
12.57 %
0
0.00 %
PAR-PERSIS
1
416
1.93 %
38
9.13 %
367
88.22 %
13
3.13 %
11
2.64 %
0
0.00 %
Devon
PAR-CUL-N
9
904
4.19 %
61
6.75 %
795
87.94 %
56
6.19 %
46
5.09 %
2
0.22 %
PAR-CUL-P
1
363
1.68 %
30
8.26 %
302
83.20 %
38
10.47 %
31
8.54 %
0
0.00 %
PAR-PERSIS
3
304
1.41 %
44
14.47 %
253
83.22 %
20
6.58 %
6
1.97 %
1
0.33 %
Dorset
PAR-CUL-N
4
541
2.51 %
51
9.43 %
468
86.51 %
35
6.47 %
22
4.07 %
0
0.00 %
Gloucs
PAR-CUL-N
5
576
2.67 %
11
1.91 %
546
94.79 %
26
4.51 %
19
3.30 %
0
0.00 %
Heref
PAR-CUL-P
2
61
0.28 %
1
1.64 %
48
78.69 %
13
21.31 %
12
19.67 %
0
0.00 %
PAR-OTHER
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
381
1.77 %
40
10.50 %
317
83.20 %
28
7.35 %
24
6.30 %
0
0.00 %
Shropshire
PAR-PERSIS
3
692
3.21 %
16
2.31 %
643
92.92 %
40
5.78 %
30
4.34 %
3
0.43 %
Somerset
PAR-CUL-N
3
381
1.77 %
16
4.20 %
358
93.96 %
7
1.84 %
7
1.84 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
6
472
2.19 %
11
2.33 %
355
75.21 %
20
4.24 %
11
2.33 %
95
20.13 %
Bucks
PAR-NEW-IN
3
889
4.12 %
70
7.87 %
766
86.16 %
62
6.97 %
53
5.96 %
0
0.00 %
Cheshire
PAR-NEW-IN
19
2,695
12.50 %
63
2.34 %
2480
92.02 %
219
8.13 %
150
5.57 %
2
0.07 %
Derbyshire
PAR-NEW-IN
15
2,013
9.34 %
117
5.81 %
1714
85.15 %
183
9.09 %
181
8.99 %
1
0.05 %
E Sussex
PAR-NEW-IN
1
293
1.36 %
13
4.44 %
273
93.17 %
7
2.39 %
7
2.39 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
397
1.84 %
62
15.62 %
315
79.35 %
44
11.08 %
18
4.53 %
2
0.50 %
Leics & Rut
PAR-NEW-IN
4
698
3.24 %
23
3.30 %
655
93.84 %
26
3.72 %
20
2.87 %
0
0.00 %
PAR-OTHER
2
24
0.11 %
3
12.50 %
19
79.17 %
1
4.17 %
1
4.17 %
1
4.17 %
Northants
PAR-NEW-IN
1
41
0.19 %
0
0.00 %
35
85.37 %
6
14.63 %
6
14.63 %
0
0.00 %
Oxon
PAR-NEW-IN
11
1,288
5.98 %
77
5.98 %
1100
85.40 %
158
12.27 %
111
8.62 %
0
0.00 %
Warks
PAR-NEW-IN
10
612
2.84 %
38
6.21 %
540
88.24 %
40
6.54 %
32
5.23 %
2
0.33 %
England - LRA
Cambs
PAR-NEW-IN
1
131
0.61 %
5
3.82 %
118
90.08 %
12
9.16 %
7
5.34 %
1
0.76 %
Cumbria
PAR-NEW-IN
3
429
1.99 %
8
1.86 %
378
88.11 %
51
11.89 %
43
10.02 %
0
0.00 %
Isle of wight
PAR-NEW-IN
1
186
0.86 %
11
5.91 %
150
80.65 %
26
13.98 %
25
13.44 %
0
0.00 %
Lincs
PAR-NEW-IN
1
8
0.04 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England
122
15274
70.86 %
824
5.39 %
13422
87.87 %
1192
7.80 %
918
6.01 %
110
0.72 %
Wales - High TB
Carms
PAR-HERD-S
8
323
1.50 %
32
9.91 %
274
84.83 %
20
6.19 %
16
4.95 %
1
0.31 %
PAR-OTHER
5
28
0.13 %
7
25.00 %
14
50.00 %
10
35.71 %
7
25.00 %
0
0.00 %
PAR-PERSIS
9
946
4.39 %
55
5.81 %
848
89.64 %
76
8.03 %
39
4.12 %
4
0.42 %
PAR-RAPID
5
17
0.08 %
5
29.41 %
12
70.59 %
1
5.88 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
156
0.72 %
24
15.38 %
129
82.69 %
7
4.49 %
3
1.92 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
299
1.39 %
26
8.70 %
256
85.62 %
18
6.02 %
15
5.02 %
2
0.67 %
SER-FLEXI
1
61
0.28 %
15
24.59 %
44
72.13 %
1
1.64 %
1
1.64 %
1
1.64 %
Gwent
PAR-PERSIS
2
97
0.45 %
11
11.34 %
76
78.35 %
10
10.31 %
8
8.25 %
2
2.06 %
PAR-RAPID
4
11
0.05 %
5
45.45 %
5
45.45 %
1
9.09 %
1
9.09 %
0
0.00 %
Pembs
PAR-HERD-S
7
906
4.20 %
46
5.08 %
820
90.51 %
56
6.18 %
34
3.75 %
6
0.66 %
PAR-OTHER
1
259
1.20 %
12
4.63 %
221
85.33 %
34
13.13 %
26
10.04 %
0
0.00 %
PAR-PERSIS
4
365
1.69 %
21
5.75 %
327
89.59 %
19
5.21 %
17
4.66 %
0
0.00 %
PAR-RAPID
10
30
0.14 %
2
6.67 %
27
90.00 %
2
6.67 %
1
3.33 %
0
0.00 %
SER-FLEXI
3
50
0.23 %
2
4.00 %
47
94.00 %
0
0.00 %
0
0.00 %
1
2.00 %
Powys
PAR-HERD-S
2
18
0.08 %
2
11.11 %
15
83.33 %
1
5.56 %
1
5.56 %
0
0.00 %
PAR-NEW-IN
2
91
0.42 %
2
2.20 %
87
95.60 %
3
3.30 %
2
2.20 %
0
0.00 %
PAR-PERSIS
2
206
0.96 %
6
2.91 %
193
93.69 %
7
3.40 %
7
3.40 %
0
0.00 %
S Wales
PAR-OTHER
2
86
0.40 %
5
5.81 %
78
90.70 %
4
4.65 %
3
3.49 %
0
0.00 %
PAR-RAPID
1
3
0.01 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
113
0.52 %
1
0.88 %
105
92.92 %
12
10.62 %
7
6.19 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
366
1.70 %
32
8.74 %
315
86.07 %
30
8.20 %
18
4.92 %
1
0.27 %
PAR-OTHER
1
402
1.87 %
61
15.17 %
321
79.85 %
22
5.47 %
20
4.98 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
2
18
0.08 %
2
11.11 %
13
72.22 %
4
22.22 %
3
16.67 %
0
0.00 %
SER-FLEXI
2
10
0.05 %
1
10.00 %
8
80.00 %
1
10.00 %
1
10.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
6
294
1.36 %
8
2.72 %
270
91.84 %
20
6.80 %
14
4.76 %
2
0.68 %
PAR-PERSIS
1
108
0.50 %
3
2.78 %
99
91.67 %
14
12.96 %
6
5.56 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
38
0.18 %
6
15.79 %
28
73.68 %
4
10.53 %
4
10.53 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
211
0.98 %
6
2.84 %
190
90.05 %
23
10.90 %
15
7.11 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
355
1.65 %
7
1.97 %
339
95.49 %
24
6.76 %
9
2.54 %
0
0.00 %
NE Wales
PAR-NEW-IN
4
408
1.89 %
10
2.45 %
382
93.63 %
26
6.37 %
15
3.68 %
1
0.25 %
Wales
106
6280
29.14 %
417
6.64 %
5549
88.36 %
450
7.17 %
293
4.67 %
21
0.33 %
Grand Total
Sum:
228
21554
1241
18971
xx-xxxx-xxxx
1211
131
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-OTHER
1
4
0.00 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-PERSIS
8
995
0.39 %
59
5.93 %
849
85.33 %
104
10.45 %
86
8.64 %
1
0.10 %
SER-FLEXI
2
401
0.16 %
55
13.72 %
309
77.06 %
43
10.72 %
37
9.23 %
0
0.00 %
SER-FRAUD
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
54
6,985
2.76 %
291
4.17 %
6349
90.89 %
537
7.69 %
344
4.92 %
1
0.01 %
PAR-CUL-P
1
10
0.00 %
0
0.00 %
8
80.00 %
4
40.00 %
2
20.00 %
0
0.00 %
PAR-NEW-IN
2
304
0.12 %
24
7.89 %
252
82.89 %
42
13.82 %
28
9.21 %
0
0.00 %
PAR-OTHER
3
228
0.09 %
6
2.63 %
209
91.67 %
21
9.21 %
13
5.70 %
0
0.00 %
PAR-PERSIS
23
4,594
1.81 %
277
6.03 %
4150
90.34 %
253
5.51 %
167
3.64 %
0
0.00 %
SER-FLEXI
9
1,179
0.47 %
96
8.14 %
1028
87.19 %
54
4.58 %
47
3.99 %
8
0.68 %
Devon
PAR-CUL-N
61
8,415
3.32 %
604
7.18 %
7276
86.46 %
683
8.12 %
406
4.82 %
129
1.53 %
PAR-CUL-P
19
5,577
2.20 %
331
5.94 %
4923
88.27 %
325
5.83 %
223
4.00 %
100
1.79 %
PAR-NEW-IN
9
904
0.36 %
67
7.41 %
785
86.84 %
62
6.86 %
40
4.42 %
12
1.33 %
PAR-OTHER
6
190
0.07 %
4
2.11 %
178
93.68 %
19
10.00 %
8
4.21 %
0
0.00 %
PAR-PERSIS
38
6,447
2.54 %
363
5.63 %
5648
87.61 %
557
8.64 %
420
6.51 %
16
0.25 %
SER-FLEXI
14
2,191
0.86 %
160
7.30 %
1967
89.78 %
162
7.39 %
61
2.78 %
3
0.14 %
Dorset
PAR-CUL-N
54
8,820
3.48 %
555
6.29 %
7804
88.48 %
627
7.11 %
458
5.19 %
3
0.03 %
PAR-CUL-P
1
271
0.11 %
13
4.80 %
239
88.19 %
26
9.59 %
19
7.01 %
0
0.00 %
PAR-NEW-IN
2
636
0.25 %
8
1.26 %
558
87.74 %
73
11.48 %
70
11.01 %
0
0.00 %
PAR-OTHER
2
98
0.04 %
7
7.14 %
80
81.63 %
15
15.31 %
11
11.22 %
0
0.00 %
PAR-PERSIS
18
8,205
3.24 %
414
5.05 %
7405
90.25 %
576
7.02 %
381
4.64 %
5
0.06 %
SER-FLEXI
1
69
0.03 %
4
5.80 %
62
89.86 %
3
4.35 %
3
4.35 %
0
0.00 %
Gloucs
PAR-CUL-N
52
4,940
1.95 %
174
3.52 %
4425
89.57 %
549
11.11 %
338
6.84 %
3
0.06 %
PAR-CUL-P
9
560
0.22 %
21
3.75 %
471
84.11 %
92
xx-xxxx-xxxx %
68
12.14 %
0
0.00 %
PAR-NEW-IN
2
186
0.07 %
5
2.69 %
160
86.02 %
27
14.52 %
21
11.29 %
0
0.00 %
PAR-OTHER
2
180
0.07 %
30
16.67 %
141
78.33 %
17
9.44 %
9
5.00 %
0
0.00 %
PAR-PERSIS
5
632
0.25 %
58
9.18 %
549
86.87 %
61
9.65 %
25
3.96 %
0
0.00 %
SER-FLEXI
5
1,342
0.53 %
45
3.35 %
1247
92.92 %
76
5.66 %
49
3.65 %
1
0.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Heref
PAR-CUL-N
23
797
0.31 %
64
8.03 %
671
84.19 %
82
10.29 %
62
7.78 %
0
0.00 %
PAR-CUL-P
4
139
0.05 %
4
2.88 %
120
86.33 %
17
12.23 %
15
10.79 %
0
0.00 %
PAR-NEW-IN
3
466
0.18 %
25
5.36 %
429
92.06 %
39
8.37 %
12
2.58 %
0
0.00 %
PAR-OTHER
1
5
0.00 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
613
0.24 %
54
8.81 %
524
85.48 %
39
6.36 %
34
5.55 %
1
0.16 %
SER-FLEXI
2
246
0.10 %
35
14.23 %
200
81.30 %
27
10.98 %
11
4.47 %
0
0.00 %
SER-FRAUD
2
38
0.01 %
1
2.63 %
36
94.74 %
2
5.26 %
1
2.63 %
0
0.00 %
Shropshire
PAR-NEW-IN
2
55
0.02 %
1
1.82 %
52
94.55 %
2
3.64 %
2
3.64 %
0
0.00 %
PAR-OTHER
8
1,142
0.45 %
64
5.60 %
1034
90.54 %
57
4.99 %
43
3.77 %
1
0.09 %
PAR-PERSIS
16
2,438
0.96 %
144
5.91 %
2199
90.20 %
151
6.19 %
92
3.77 %
3
0.12 %
Somerset
PAR-CUL-N
22
1,443
0.57 %
66
4.57 %
1268
87.87 %
64
4.44 %
57
3.95 %
52
3.60 %
PAR-CUL-P
4
153
0.06 %
14
9.15 %
130
84.97 %
37
24.18 %
9
5.88 %
0
0.00 %
PAR-NEW-IN
1
22
0.01 %
1
4.55 %
16
72.73 %
5
22.73 %
5
22.73 %
0
0.00 %
PAR-OTHER
6
608
0.24 %
41
6.74 %
437
71.88 %
24
3.95 %
22
3.62 %
108
17.76 %
PAR-PERSIS
21
4,736
1.87 %
413
8.72 %
4098
86.53 %
299
6.31 %
204
4.31 %
21
0.44 %
Staffs
PAR-PERSIS
3
640
0.25 %
17
2.66 %
588
91.88 %
33
5.16 %
18
2.81 %
17
2.66 %
SER-FLEXI
4
969
0.38 %
103
10.63 %
755
77.92 %
153
15.79 %
110
11.35 %
1
0.10 %
Wiltshire
PAR-NEW-IN
2
352
0.14 %
33
9.38 %
308
87.50 %
26
7.39 %
11
3.13 %
0
0.00 %
PAR-OTHER
5
703
0.28 %
54
7.68 %
610
86.77 %
55
7.82 %
22
3.13 %
17
2.42 %
PAR-PERSIS
12
1,731
0.68 %
71
4.10 %
1605
92.72 %
78
4.51 %
55
3.18 %
0
0.00 %
SER-FLEXI
6
153
0.06 %
19
12.42 %
131
85.62 %
4
2.61 %
3
1.96 %
0
0.00 %
SER-FRAUD
2
9
0.00 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Midlands
PAR-PERSIS
1
281
0.11 %
1
0.36 %
265
94.31 %
16
5.69 %
14
4.98 %
1
0.36 %
SER-FRAUD
3
120
0.05 %
2
1.67 %
111
92.50 %
10
8.33 %
7
5.83 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
41
3,559
1.40 %
159
4.47 %
3095
86.96 %
367
10.31 %
210
5.90 %
95
2.67 %
PAR-PERSIS
6
105
0.04 %
6
5.71 %
95
90.48 %
7
6.67 %
4
3.81 %
0
0.00 %
Bucks
PAR-NEW-IN
22
3,513
1.39 %
191
5.44 %
3037
86.45 %
205
5.84 %
152
4.33 %
133
3.79 %
Cheshire
PAR-NEW-IN
216
38,469
15.18 %
1299
3.38 %
35043
91.09 %
3038
7.90 %
2121
5.51 %
6
0.02 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
10
2,218
0.87 %
139
6.27 %
1770
79.80 %
235
10.60 %
207
9.33 %
102
4.60 %
Derbyshire
PAR-NEW-IN
128
15,747
6.21 %
624
3.96 %
14055
89.26 %
1509
9.58 %
1064
6.76 %
4
0.03 %
E Sussex
PAR-NEW-IN
27
3,324
1.31 %
146
4.39 %
2983
89.74 %
304
9.15 %
192
5.78 %
3
0.09 %
PAR-PERSIS
2
158
0.06 %
10
6.33 %
142
89.87 %
16
10.13 %
6
3.80 %
0
0.00 %
Hampshire
PAR-NEW-IN
32
3,156
1.25 %
165
5.23 %
2823
89.45 %
257
8.14 %
166
5.26 %
2
0.06 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-PERSIS
1
19
0.01 %
1
5.26 %
16
84.21 %
7
36.84 %
2
10.53 %
0
0.00 %
SER-NSR
3
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
45
4,381
1.73 %
194
4.43 %
3918
89.43 %
371
8.47 %
267
6.09 %
2
0.05 %
PAR-OTHER
4
51
0.02 %
4
7.84 %
45
88.24 %
2
3.92 %
1
1.96 %
1
1.96 %
SER-NSR
1
13
0.01 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Northants
PAR-NEW-IN
28
1,575
0.62 %
79
5.02 %
1407
89.33 %
130
8.25 %
89
5.65 %
0
0.00 %
Notts
PAR-NEW-IN
21
1,503
0.59 %
51
3.39 %
1339
89.09 %
152
10.11 %
113
7.52 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
110
11,391
4.49 %
686
6.02 %
9919
87.08 %
1131
9.93 %
777
6.82 %
9
0.08 %
PAR-PERSIS
14
2,054
0.81 %
96
4.67 %
1861
90.60 %
121
5.89 %
97
4.72 %
0
0.00 %
Warks
PAR-NEW-IN
89
7,071
2.79 %
354
5.01 %
6205
87.75 %
629
8.90 %
431
6.10 %
81
1.15 %
PAR-PERSIS
2
23
0.01 %
1
4.35 %
19
82.61 %
3
13.04 %
3
13.04 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
561
0.22 %
12
2.14 %
453
80.75 %
124
22.10 %
96
17.11 %
0
0.00 %
Cambs
PAR-NEW-IN
1
131
0.05 %
5
3.82 %
118
90.08 %
12
9.16 %
7
5.34 %
1
0.76 %
Cumbria
PAR-NEW-IN
40
5,744
2.27 %
187
3.26 %
5196
90.46 %
510
8.88 %
361
6.28 %
0
0.00 %
PAR-OTHER
3
276
0.11 %
30
10.87 %
205
74.28 %
50
18.12 %
41
14.86 %
0
0.00 %
PAR-PERSIS
1
330
0.13 %
11
3.33 %
304
92.12 %
18
5.45 %
15
4.55 %
0
0.00 %
Essex
PAR-NEW-IN
3
493
0.19 %
31
6.29 %
456
92.49 %
26
5.27 %
3
0.61 %
3
0.61 %
Herts
PAR-NEW-IN
4
366
0.14 %
5
1.37 %
325
88.80 %
61
16.67 %
36
9.84 %
0
0.00 %
Humberside
PAR-NEW-IN
2
99
0.04 %
8
8.08 %
86
86.87 %
8
8.08 %
5
5.05 %
0
0.00 %
Isle of wight
PAR-NEW-IN
5
741
0.29 %
32
4.32 %
447
60.32 %
83
11.20 %
62
8.37 %
200
26.99 %
Kent
PAR-NEW-IN
3
649
0.26 %
33
5.08 %
599
92.30 %
37
5.70 %
17
2.62 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Lincs
PAR-NEW-IN
10
374
0.15 %
26
6.95 %
316
84.49 %
42
11.23 %
32
8.56 %
0
0.00 %
Merseyside
PAR-NEW-IN
2
34
0.01 %
0
0.00 %
20
58.82 %
15
44.12 %
14
41.18 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
455
0.18 %
9
1.98 %
409
89.89 %
48
10.55 %
37
8.13 %
0
0.00 %
N Yorks
PAR-NEW-IN
6
942
0.37 %
30
3.18 %
866
91.93 %
79
8.39 %
46
4.88 %
0
0.00 %
S Yorks
PAR-NEW-IN
2
131
0.05 %
11
8.40 %
110
83.97 %
10
7.63 %
10
7.63 %
0
0.00 %
W Sussex
PAR-NEW-IN
11
1,442
0.57 %
81
5.62 %
1300
90.15 %
87
6.03 %
61
4.23 %
0
0.00 %
PAR-PERSIS
2
297
0.12 %
6
2.02 %
277
93.27 %
15
5.05 %
14
4.71 %
0
0.00 %
England
1469
193638
76.39 %
9620
4.97 % ######
88.81 %
15940
8.23 %
10905
5.63 %
1146
0.59 %
Wales - High TB
Carms
PAR-HERD-S
46
1,567
0.62 %
123
7.85 %
1361
86.85 %
122
7.79 %
79
5.04 %
4
0.26 %
PAR-OTHER
12
170
0.07 %
21
12.35 %
136
80.00 %
17
10.00 %
13
7.65 %
0
0.00 %
PAR-PERSIS
61
6,052
2.39 %
461
7.62 %
5228
86.38 %
506
8.36 %
344
5.68 %
19
0.31 %
PAR-RAPID
97
467
0.18 %
118
25.27 %
301
64.45 %
29
6.21 %
19
4.07 %
29
6.21 %
SER-FLEXI
17
540
0.21 %
63
11.67 %
421
77.96 %
65
12.04 %
52
9.63 %
4
0.74 %
Ceredigion
PAR-HERD-S
2
6
0.00 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
PAR-OTHER
1
9
0.00 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
18
1,800
0.71 %
123
6.83 %
1584
88.00 %
104
5.78 %
87
4.83 %
6
0.33 %
PAR-RAPID
32
115
0.05 %
23
20.00 %
91
79.13 %
6
5.22 %
1
0.87 %
0
0.00 %
SER-FLEXI
11
326
0.13 %
40
12.27 %
258
79.14 %
31
9.51 %
24
7.36 %
4
1.23 %
Gwent
PAR-HERD-S
5
429
0.17 %
16
3.73 %
395
92.07 %
36
8.39 %
16
3.73 %
2
0.47 %
PAR-NEW-IN
2
17
0.01 %
1
5.88 %
13
76.47 %
4
23.53 %
3
17.65 %
0
0.00 %
PAR-OTHER
2
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
40
2,374
0.94 %
131
5.52 %
2058
86.69 %
316
13.31 %
180
7.58 %
5
0.21 %
PAR-RAPID
41
206
0.08 %
41
19.90 %
149
72.33 %
16
7.77 %
16
7.77 %
0
0.00 %
SER-FLEXI
6
205
0.08 %
3
1.46 %
179
87.32 %
37
18.05 %
23
11.22 %
0
0.00 %
Pembrokshire
PAR-OTHER
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Pembs
PAR-HERD-S
41
4,038
1.59 %
129
3.19 %
3362
83.26 %
250
6.19 %
176
4.36 %
371
9.19 %
PAR-OTHER
6
296
0.12 %
21
7.09 %
249
84.12 %
34
11.49 %
26
8.78 %
0
0.00 %
PAR-PERSIS
91
7,579
2.99 %
319
4.21 %
6584
86.87 %
583
7.69 %
417
5.50 %
259
3.42 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
143
473
0.19 %
112
23.68 %
340
71.88 %
29
6.13 %
21
4.44 %
0
0.00 %
SER-FLEXI
32
983
0.39 %
77
7.83 %
782
79.55 %
81
8.24 %
57
5.80 %
67
6.82 %
SER-FRAUD
2
100
0.04 %
55
55.00 %
45
45.00 %
1
1.00 %
0
0.00 %
0
0.00 %
Powys
PAR-HERD-S
18
689
0.27 %
77
11.18 %
570
82.73 %
50
7.26 %
41
5.95 %
1
0.15 %
PAR-NEW-IN
24
943
0.37 %
22
2.33 %
884
93.74 %
51
5.41 %
35
3.71 %
2
0.21 %
PAR-OTHER
5
109
0.04 %
3
2.75 %
103
94.50 %
4
3.67 %
3
2.75 %
0
0.00 %
PAR-PERSIS
50
4,127
1.63 %
163
3.95 %
3543
85.85 %
299
7.24 %
248
6.01 %
173
4.19 %
PAR-RAPID
35
124
0.05 %
18
14.52 %
94
75.81 %
13
10.48 %
11
8.87 %
1
0.81 %
SER-FLEXI
6
101
0.04 %
3
2.97 %
93
92.08 %
5
4.95 %
5
4.95 %
0
0.00 %
S Wales
PAR-OTHER
2
86
0.03 %
5
5.81 %
78
90.70 %
4
4.65 %
3
3.49 %
0
0.00 %
PAR-PERSIS
3
8
0.00 %
1
12.50 %
5
62.50 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-RAPID
4
11
0.00 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
113
0.04 %
1
0.88 %
105
92.92 %
12
10.62 %
7
6.19 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
366
0.14 %
32
8.74 %
315
86.07 %
30
8.20 %
18
4.92 %
1
0.27 %
PAR-OTHER
2
555
0.22 %
77
13.87 %
447
80.54 %
34
6.13 %
30
5.41 %
1
0.18 %
PAR-PERSIS
2
289
0.11 %
48
16.61 %
219
75.78 %
25
8.65 %
22
7.61 %
0
0.00 %
PAR-RAPID
5
25
0.01 %
6
24.00 %
16
64.00 %
3
12.00 %
3
12.00 %
0
0.00 %
Ceredigion
PAR-HERD-S
11
1,019
0.40 %
28
2.75 %
810
79.49 %
237
23.26 %
180
17.66 %
1
0.10 %
PAR-NEW-IN
1
68
0.03 %
4
5.88 %
63
92.65 %
3
4.41 %
1
1.47 %
0
0.00 %
PAR-PERSIS
3
153
0.06 %
22
14.38 %
116
75.82 %
24
15.69 %
15
9.80 %
0
0.00 %
PAR-RAPID
21
91
0.04 %
21
23.08 %
67
73.63 %
4
4.40 %
3
3.30 %
0
0.00 %
SER-FLEXI
7
203
0.08 %
12
5.91 %
174
85.71 %
19
9.36 %
17
8.37 %
0
0.00 %
Mid glamorgan
PAR-PERSIS
2
106
0.04 %
2
1.89 %
80
75.47 %
30
28.30 %
24
22.64 %
0
0.00 %
NE Wales
PAR-HERD-S
1
17
0.01 %
0
0.00 %
13
76.47 %
4
23.53 %
4
23.53 %
0
0.00 %
PAR-NEW-IN
133
12,295
4.85 %
219
1.78 %
11323
92.09 %
944
7.68 %
691
5.62 %
62
0.50 %
PAR-PERSIS
4
216
0.09 %
6
2.78 %
197
91.20 %
22
10.19 %
13
6.02 %
0
0.00 %
PAR-RAPID
11
33
0.01 %
6
18.18 %
25
75.76 %
2
6.06 %
1
3.03 %
1
3.03 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2018 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
11
209
0.08 %
19
9.09 %
177
84.69 %
14
6.70 %
13
6.22 %
0
0.00 %
Powys
PAR-HERD-S
1
1
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
PAR-NEW-IN
8
398
0.16 %
15
3.77 %
364
91.46 %
38
9.55 %
19
4.77 %
0
0.00 %
PAR-RAPID
3
8
0.00 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
5
257
0.10 %
11
4.28 %
229
89.11 %
25
9.73 %
17
6.61 %
0
0.00 %
SER-FLEXI
2
5
0.00 %
0
0.00 %
4
80.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
24
2,170
0.86 %
53
2.44 %
1960
90.32 %
177
8.16 %
103
4.75 %
54
2.49 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
42
4,983
1.97 %
107
2.15 %
4441
89.12 %
370
7.43 %
275
5.52 %
160
3.21 %
PAR-OTHER
1
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
5
0.00 %
0
0.00 %
2
40.00 %
3
60.00 %
3
60.00 %
0
0.00 %
PAR-RAPID
3
14
0.01 %
2
14.29 %
12
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
46
0.02 %
0
0.00 %
45
97.83 %
2
4.35 %
1
2.17 %
0
0.00 %
Wales
1169
57607
22.73 %
2869
4.98 %
50144
87.04 %
4721
8.20 %
3366
5.84 %
1228
2.13 %
Scotland - Scotland
Aberdeenshire
PAR-NEW-IN
2
15
0.01 %
0
0.00 %
14
93.33 %
1
6.67 %
1
6.67 %
0
0.00 %
Argyll & Bute
PAR-NEW-IN
12
887
0.35 %
68
7.67 %
766
86.36 %
77
8.68 %
53
5.98 %
0
0.00 %
SER-FLEXI
1
10
0.00 %
1
10.00 %
8
80.00 %
1
10.00 %
1
10.00 %
0
0.00 %
Ayrshire
PAR-NEW-IN
7
579
0.23 %
37
6.39 %
496
85.66 %
66
11.40 %
46
7.94 %
0
0.00 %
PAR-OTHER
1
46
0.02 %
39
84.78 %
7
15.22 %
1
2.17 %
0
0.00 %
0
0.00 %
Perthshire
PAR-NEW-IN
3
217
0.09 %
17
7.83 %
187
86.18 %
22
10.14 %
13
5.99 %
0
0.00 %
West lothian
PAR-NEW-IN
2
142
0.06 %
1
0.70 %
137
96.48 %
4
2.82 %
4
2.82 %
0
0.00 %
Wigtown
PAR-NEW-IN
3
345
0.14 %
18
5.22 %
306
88.70 %
29
8.41 %
21
6.09 %
0
0.00 %
Scotland
31
2241
0.88 %
181
8.08 %
1921
85.72 %
201
8.97 %
139
6.20 %
0
0.00 %
Grand Total
Sum:
xx-xxxx-xxxx
12670
######
20862
14410
2374
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol December 2018
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
122
15274
100.0%
824
5.4%
13422
87.9%
1192
7.8%
918
6.0%
110
0.7%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
122
15274
100.0%
824
5.4%
13422
87.9%
1192
7.8%
918
6.0%
110
0.7%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
93
5965
95.0%
369
6.2%
5293
88.7%
437
7.3%
284
4.8%
19
0.3%
Serial/Extended
13
315
5.0%
48
15.2%
256
81.3%
13
4.1%
9
2.9%
2
0.6%
Total
106
6280
100.0%
417
6.6%
5549
88.4%
450
7.2%
293
4.7%
21
0.3%
GB
Parallel
215
21239
98.5%
1193
5.6%
18715
88.1%
1629
7.7%
1202
5.7%
129
0.6%
Serial/Extended
13
315
1.5%
48
15.2%
256
81.3%
13
4.1%
9
2.9%
2
0.6%
Total
228
21554
100.0%
1241
5.8%
18971
88.0%
xx-xxxx-xxxx
7.6%
1211
5.6%
131
0.6%
122.00
106.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2018)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
96.5%
9100
4.9% 166080
88.9%
15406
8.2%
10576
5.7%
1133
0.6%
Serial/Extended
55
6749
3.5%
520
7.7%
5887
87.2%
534
7.9%
329
4.9%
13
0.2%
Total
1469 193638
100.0%
9620
5.0% 171967
88.8%
15940
8.2%
10905
5.6%
1146
0.6%
Scotland
Parallel
30
2231
99.6%
180
8.1%
1913
85.7%
200
9.0%
138
6.2%
0
0.0 %
Serial/Extended
1
10
0.4%
1
0.0%
8
80.0%
1
10.0%
1
10.0%
0
0.0 %
Total
31
2241
100.0%
181
8.1%
1921
85.7%
201
9.0%
139
6.2%
0
0.0 %
Wales
Parallel
1073
54889
95.3%
2597
4.7%
47966
87.4%
4465
8.1%
3173
5.8%
1153
2.1%
Serial/Extended
96
2718
4.7%
272
10.0%
2178
80.1%
256
9.4%
193
7.1%
75
2.8%
Total
1169
57607
100.0%
2869
5.0%
50144
87.0%
4721
8.2%
3366
5.8%
1228
2.1%
GB
Parallel
xx-xxxx-xxxx
96.3% 11877
4.9% 215959
88.5%
20071
8.2%
13887
5.7%
2286
0.9%
Serial/Extended
152
9477
3.7%
793
8.4%
8073
85.2%
791
8.3%
523
5.5%
88
0.9%
Total
xx-xxxx-xxxx
100.0% 12670
5.0% 224032
88.4%
20862
8.2%
14410
5.7%
2374
0.9%
#######
#######
31.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
315
1.5%
48
15.2%
256
81.3%
13
4.1 %
9
2.9 %
2
0.6 %
Parallel - Herd or Group Slaughter
21
1631
7.6%
114
7.0%
1437
88.1%
111
6.8 %
72
4.4 %
8
0.5 %
Parallel New Infection
98
11486
53.3%
535
4.7%
10118
88.1%
955
8.3 %
727
6.3 %
106
0.9 %
Parallel - new infection cull area
28
2693
12.5%
144
5.3%
2431
90.3%
157
5.8 %
116
4.3 %
2
0.1 %
Parallel - Other
15
1128
5.2%
95
8.4%
953
84.5%
106
9.4 %
79
7.0 %
1
0.1 %
Parallel Persistent Infection
28
3814
17.7%
260
6.8%
3379
88.6%
245
6.4 %
163
4.3 %
12
0.3 %
Parallel - persistent infection cull area
3
424
2.0%
31
7.3%
350
82.5%
51
12.0 %
43
10.1 %
0
Parallel - Rapid Testing Two-times IRs
22
63
0.3%
14
22.2%
47
74.6%
4
6.3 %
2
3.2 %
0
Total
228
21554
100.0%
1241
5.8%
18971
88.0%
xx-xxxx-xxxx
7.6 %
1211
5.6 %
131
0.6 %
Table 4a. Summaryof IFN Gamma results by submission reason (December 2018)
England - HRA
Parallel New Infection
1
183
0.8%
6
3.3%
154
84.2%
28
15.3 %
23
12.6 %
0
Parallel - new infection cull area
28
2693
12.5%
144
5.3%
2431
90.3%
157
5.8 %
116
4.3 %
2
0.1 %
Parallel - Other
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Parallel Persistent Infection
9
1793
8.3%
138
7.7%
1580
88.1%
101
5.6 %
71
4.0 %
4
0.2 %
Parallel - persistent infection cull area
3
424
2.0%
31
7.3%
350
82.5%
51
12.0 %
43
10.1 %
0
England - HRA
42
5098
23.7%
319
6.3%
4520
88.7%
337
6.6 %
253
5.0 %
6
0.1 %
England - Edge
Parallel New Infection
72
9398
43.6%
474
5.0%
8233
87.6%
765
8.1 %
589
6.3 %
102
1.1 %
Parallel - Other
2
24
0.1%
3
12.5%
19
79.2%
1
4.2 %
1
4.2 %
1
4.2 %
England - Edge
74
9422
43.7%
477
5.1%
8252
87.6%
766
8.1 %
590
6.3 %
103
1.1 %
England - LRA
Parallel New Infection
6
754
3.5%
28
3.7%
650
86.2%
89
11.8 %
75
9.9 %
1
0.1 %
England - LRA
6
754
3.5%
28
3.7%
650
86.2%
89
11.8 %
75
9.9 %
1
0.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
9
267
1.2%
41
15.4%
220
82.4%
8
3.0 %
4
1.5 %
2
0.7 %
Parallel - Herd or Group Slaughter
17
1247
5.8%
80
6.4%
1109
88.9%
77
6.2 %
51
4.1 %
7
0.6 %
Parallel New Infection
2
91
0.4%
2
2.2%
87
95.6%
3
3.3 %
2
2.2 %
0
Parallel - Other
9
486
2.3%
25
5.1%
418
86.0%
60
12.3 %
43
8.8 %
0
Parallel Persistent Infection
18
1913
8.9%
119
6.2%
1700
88.9%
130
6.8 %
86
4.5 %
8
0.4 %
Parallel - Rapid Testing Two-times IRs
20
61
0.3%
13
21.3%
46
75.4%
4
6.6 %
2
3.3 %
0
Wales - High TB
75
4065
18.9%
280
6.9%
3580
88.1%
282
6.9 %
188
4.6 %
17
0.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
48
0.2%
7
14.6%
36
75.0%
5
10.4 %
5
10.4 %
0
Parallel - Herd or Group Slaughter
4
384
1.8%
34
8.9%
328
85.4%
34
8.9 %
21
5.5 %
1
0.3 %
Parallel New Infection
7
297
1.4%
8
2.7%
273
91.9%
20
6.7 %
14
4.7 %
2
0.7 %
Parallel - Other
3
613
2.8%
67
10.9%
511
83.4%
45
7.3 %
35
5.7 %
0
Parallel Persistent Infection
1
108
0.5%
3
2.8%
99
91.7%
14
13.0 %
6
5.6 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales - Intermediate TB
21
1452
6.7%
120
8.3%
1248
86.0%
118
8.1 %
81
5.6 %
3
0.2 %
Wales - Low TB
Parallel New Infection
10
763
3.5%
17
2.2%
721
94.5%
50
6.6 %
24
3.1 %
1
0.1 %
Wales - Low TB
10
763
3.5%
17
2.2%
721
94.5%
50
6.6 %
24
3.1 %
1
0.1 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
138
9178
3.6%
735
8.0%
7840
85.4%
778
8.5 %
515
5.6 %
88
1.0 %
Parallel - Herd or Group Slaughter
127
8132
3.2%
405
5.0%
6830
84.0%
731
9.0 %
516
6.3 %
381
4.7 %
Parallel New Infection
xx-xxxx-xxxx
52.0%
5144
3.9%
118042
89.5%
11297
8.6 %
7823
5.9 %
829
0.6 %
Parallel - new infection cull area
266
31400
12.4%
1754
5.6%
27793
88.5%
2542
8.1 %
1665
5.3 %
188
0.6 %
Parallel - Other
82
5134
2.0%
422
8.2%
4311
84.0%
395
7.7 %
273
5.3 %
128
2.5 %
Parallel Persistent Infection
464
59225
23.4%
3417
5.8%
51980
87.8%
4503
7.6 %
3199
5.4 %
629
1.1 %
Parallel - persistent infection cull area
38
6710
2.6%
383
5.7%
5891
87.8%
501
7.5 %
336
5.0 %
100
1.5 %
Parallel - Rapid Testing Two-times IRs
398
1570
0.6%
352
22.4%
1112
70.8%
102
6.5 %
75
4.8 %
31
2.0 %
Serial - Anomalous Reaction
10
270
0.1%
58
21.5%
204
75.6%
13
4.8 %
8
3.0 %
0
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
Total
xx-xxxx-xxxx
100.0%
12670
5.0%
224032
88.4%
20862
8.2 %
14410
5.7 %
2374
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2018)
England - HRA
Extended Flexible IFN-GAMMA test
43
6550
2.6%
517
7.9%
5699
87.0%
522
8.0 %
321
4.9 %
13
0.2 %
Parallel New Infection
23
2925
1.2%
164
5.6%
2560
87.5%
276
9.4 %
189
6.5 %
12
0.4 %
Parallel - new infection cull area
266
31400
12.4%
1754
5.6%
27793
88.5%
2542
8.1 %
1665
5.3 %
188
0.6 %
Parallel - Other
34
3158
1.2%
206
6.5%
2696
85.4%
210
6.6 %
130
4.1 %
126
4.0 %
Parallel Persistent Infection
151
31312
12.4%
1871
6.0%
27880
89.0%
2167
6.9 %
1496
4.8 %
65
0.2 %
Parallel - persistent infection cull area
38
6710
2.6%
383
5.7%
5891
87.8%
501
7.5 %
336
5.0 %
100
1.5 %
Serial - Anomalous Reaction
8
170
0.1%
3
1.8%
159
93.5%
12
7.1 %
8
4.7 %
0
England - Edge
Parallel New Infection
759
93689
37.0%
3948
4.2%
83824
89.5%
8093
8.6 %
5582
6.0 %
335
0.4 %
Parallel - Other
6
53
0.0%
4
7.5%
46
86.8%
3
5.7 %
2
3.8 %
1
1.9 %
Parallel Persistent Infection
35
4577
1.8%
253
5.5%
3903
85.3%
389
8.5 %
319
7.0 %
102
2.2 %
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
England - LRA
Parallel New Infection
96
12162
4.8%
470
3.9%
10701
88.0%
1142
9.4 %
787
6.5 %
204
1.7 %
Parallel - Other
3
276
0.1%
30
10.9%
205
74.3%
50
18.1 %
41
14.9 %
0
Parallel Persistent Infection
3
627
0.2%
17
2.7%
581
92.7%
33
5.3 %
29
4.6 %
0
England
1469 193638
76.4%
9620
5.0%
171967
88.8%
15940
8.2 %
10905
5.6 %
1146
0.6 %

Table 4b. Summaryof IFN Gamma results by submission reason (Total 2018) Cont.
Wales - High TB
Extended Flexible IFN-GAMMA test
72
2155
0.9%
186
8.6%
1733
80.4%
219
10.2 %
161
7.5 %
75
3.5 %
Parallel - Herd or Group Slaughter
112
6729
2.7%
345
5.1%
5692
84.6%
460
6.8 %
314
4.7 %
378
5.6 %
Parallel New Infection
26
960
0.4%
23
2.4%
897
93.4%
55
5.7 %
38
4.0 %
2
0.2 %
Parallel - Other
30
787
0.3%
54
6.9%
680
86.4%
72
9.1 %
53
6.7 %
0
Parallel Persistent Infection
263
21940
8.7%
1198
5.5%
19002
86.6%
1810
8.2 %
1278
5.8 %
462
2.1 %
Parallel - Rapid Testing Two-times IRs
354
1398
0.6%
315
22.5%
985
70.5%
93
6.7 %
68
4.9 %
30
2.1 %
Serial - Anomalous Reaction
2
100
0.0%
55
55.0%
45
45.0%
1
1.0 %
0
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
20
417
0.2%
31
7.4%
355
85.1%
34
8.2 %
31
7.4 %
0
Parallel - Herd or Group Slaughter
15
1403
0.6%
60
4.3%
1138
81.1%
271
19.3 %
202
14.4 %
3
0.2 %
Parallel New Infection
143
12764
5.0%
238
1.9%
11753
92.1%
985
7.7 %
711
5.6 %
62
0.5 %
Parallel - Other
7
812
0.3%
88
10.8%
676
83.3%
59
7.3 %
47
5.8 %
1
0.1 %
Parallel Persistent Infection
11
764
0.3%
78
10.2%
612
80.1%
101
13.2 %
74
9.7 %
0
Parallel - Rapid Testing Two-times IRs
40
157
0.1%
35
22.3%
114
72.6%
9
5.7 %
7
4.5 %
1
0.6 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
46
0.0%
0
0.0 %
45
97.8%
2
4.3 %
1
2.2 %
0
Parallel New Infection
66
7153
2.8%
160
2.2%
6401
89.5%
547
7.6 %
378
5.3 %
214
3.0 %
Parallel - Other
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
2
40.0%
3
60.0 %
3
60.0 %
0
Parallel - Rapid Testing Two-times IRs
4
15
0.0%
2
13.3%
13
86.7%
0
0
0
Wales
1169
57607
22.7%
2869
5.0%
50144
87.0%
4721
8.2 %
3366
5.8 %
1228
2.1 %
Scotland - Scotland
Extended Flexible IFN-GAMMA test
1
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Parallel New Infection
29
2185
0.9%
141
6.5%
1906
87.2%
199
9.1 %
138
6.3 %
0
Parallel - Other
1
46
0.0%
39
84.8%
7
15.2%
1
2.2 %
0
0
Scotland
31
2241
0.9%
181
8.1%
1921
85.7%
201
9.0 %
139
6.2 %
0

Table 5a. Summary of PM and culture results for IFNg positive animals.
Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2018 January
207
19678
140
969
5
3
0
0
0
0
80
3
0
5
8
756
0
84
0
24
February
206
20791
130
1229
3
22
0
0
0
0
72
7
1
8
0
1073
1
35
0
5
March
189
17292
131
1199
1
3
0
0
0
0
121
6
0
4
0
984
0
72
1
2
April
195
20517
118
953
4
16
0
0
0
0
116
1
0
0
0
748
0
66
1
1
May
250
21066
131
947
2
2
0
0
0
0
121
1
0
4
0
780
0
35
1
1
June
187
18891
110
701
2
2
0
0
0
0
78
2
0
12
0
579
0
25
0
0
July
207
24111
119
806
1
12
0
0
0
0
67
4
0
2
0
675
3
38
0
2
August
228
19599
120
682
4
3
0
0
0
0
64
2
0
7
2
569
0
31
0
0
September
216
21441
137
946
6
6
0
0
0
0
33
0
0
2
1
840
3
46
1
6
October
274
23976
152
1417
12
8
0
0
0
0
99
1
0
3
1
1231
17
27
0
2
November
282
24570
192
1580
2
84
0
0
0
0
42
0
11
4
0
1255
173
1
0
0
December
228
21554
147
1241
7
808
0
0
0
0
49
0
2
0
0
330
35
0
0
0
Totals (last
12 months)
2669
253486
1627
12670
49
969
0
0
0
0
942
27
14
51
12
9820
232
460
4
43

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)
Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1469
193638
1013
9620
710
0
0
0
0
780
16
12
33
1
7939
25
43
1
2
Scotland
31
2241
20
181
30
0
0
0
0
22
4
0
0
8
50
0
37
0
27
Wales
1169
57607
594
2869
229
0
0
0
0
140
7
2
18
3
1831
207
380
3
14
Totals
2669
253486
1627
12670
969
0
0
0
0
942
27
14
51
12
9820
232
460
4
43

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
58
85
36
86
46
93
38
83
74
103
54
72
81
81
53
97
56
94
52
96
49
108
33
81
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2017
2018
IFNg Positive herds
IFNg Negative herds
9059
420
9793
442
10459
573
10179
906
13951
615
11775
419
11040
437
10097
560
10063
664
13635
971
9492
961
8778
66818401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2017
2018
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2017
2018
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
W Glamorgan
Warks
S Wales
Somerset
Shropshire
Powys
Pembs
Oxon
Northants
NE Wales
Lincs
Leics & Rut
Isle of wight
Heref
Hampshire
Gwynedd
Gwent
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Cambs
Bucks
Berks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during December 2018 (% submission per
County)
County
%Resampled %Retested
Berks
2.33 %
4.24 %
Bucks
5.96 %
6.97 %
Cambs
5.34 %
9.16 %
Carms
4.60 %
7.41 %
Ceredigion
5.15 %
6.19 %
Cheshire
5.57 %
8.13 %
Corn & Scilly
6.29 %
8.31 %
Cumbria
10.02 %
11.89 %
Derbyshire
8.99 %
9.09 %
Devon
5.28 %
7.26 %
Dorset
4.07 %
6.47 %
E Sussex
2.39 %
2.39 %
Gloucs
3.30 %
4.51 %
Gwent
8.33 %
10.19 %
Gwynedd
2.54 %
6.76 %
Hampshire
4.53 %
11.08 %
Heref
8.05 %
9.17 %
Isle of wight
13.44 %
13.98 %
Leics & Rut
2.91 %
3.74 %
Lincs
NE Wales
4.59 %
7.54 %
Northants
14.63 %
14.63 %
Oxon
8.62 %
12.27 %
Pembs
4.84 %
6.89 %
Powys
3.17 %
3.49 %
Shropshire
4.32 %
5.76 %
Somerset
1.84 %
1.84 %
S Wales
3.37 %
4.49 %
Warks
5.23 %
6.54 %
W Glamorgan
6.79 %
10.80 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
15080
824
5.5%
15274
824
5.4%
Wales
6199
417
6.7%
6280
417
6.6%
GB
21279
1241
5.8%
21554
1241
5.8%
Table 6a (i): Number of animals and submissions tested in December 2018
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
165901
9620
5.8%
193638
9620
5.0%
Wales
50414
2858
5.7%
57607
2869
5.0%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
218375
12659
5.8%
253486
12670
5.0%
Table 6b (i): Number of animals and submissions tested in 2018 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
7195
444
6.2%
7199
444
6.2%
Wales
1765
165
9.3%
1793
165
9.2%
Scotland
486
59
12.1%
486
59
12.1%
GB
9446
668
7.1%
9478
668
7.0%
Table 6a (ii): Number of animals and submissions tested in December 2017
Table 6b (ii): Number of animals and submissions tested in 2017 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
77307
5094
6.6%
98529
5094
5.2%
Wales
32133
2314
7.2%
35660
2314
6.5%
Scotland
2979
228
7.7%
3267
228
7.0%
GB
112416
7636
6.8%
137456
7636
5.6%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Northern Somerset & South Glou
PRV-SE
1
1
0
Oxfordshire
PRV-SE
1
2
1
50.00 %
Somerset excl North
PRV-SE
1
30
8
26.67 %
Total
3
33
9
27.27 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples December 2018
Table 7b Number of private samples 2018
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
2
0
Buckinghamshire
PRV-SE
1
1
0
Cheshire
PRV-SP
3
3
1
33.33 %
Cornwall and Isles of Scilly
PRV-SE
1
3
0
PRV-SP
1
1
0
Devon
PRV-SE
1
2
1
50.00 %
PRV-SP
1
2
0
Dorset
PRV-SE
4
25
6
24.00 %
PRV-SP
1
1
0
East Sussex
PRV-SE
1
1
0
PRV-SP
1
1
0
Hampshire
PRV-SE
2
6
1
16.67 %
PRV-SP
1
3
0
Northern Somerset & South Glou
PRV-SE
3
47
8
17.02 %
Oxfordshire
PRV-SE
2
40
5
12.50 %
Somerset excl North
PRV-SE
4
76
11
14.47 %
PRV-SP
1
1
0
Suffolk
PRV-SP
2
11
0
West Sussex
PRV-SP
1
1
0
Total
32
227
33
14.54 %
Isle of Man
SER-IOM
18
51
1
1.96 %
Isle of Man
SER-IOM
2
2
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
1
10
1
10.0 %
Staffordshire
1
382
91
23.8 %
TC0077
Sum:
392
92
23.5 %
Table 8a Number of Cattle Antibody Tests December 2018
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Argyll and Bute
1
3
3
100.0 %
Carmarthenshire
4
192
6
3.1 %
Ceredigion
1
85
4
4.7 %
North East Wales
2
66
4
6.1 %
Pembrokeshire
3
201
10
5.0 %
Powys
2
361
4
1.1 %
South Wales
5
199
17
8.5 %
Staffordshire
1
382
91
23.8 %
TC0077
Sum:
1,489
139
9.3 %
Table 8b Number of Cattle Antibody Tests 2018