Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - March 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 175
Report Period 1st - 31st March 2021
Issued 22 April 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
Parallel blood testing of skin test-negative cattle
in breakdowns herds occurring within 6 months
of lifting restrictions after a previous breakdown
NO (APHA discretion -
Wales only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Test Criteria

Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
March 2021
%
Total 2021
%
Submissions
419
1146
Samples
Total (%)
36344
100.00 %
93043
100.00 %
England
26938
74.12 %
72279
77.68 %
Wales
9402
25.87 %
20339
21.86 %
Scotland
4
0.01 %
425
0.46 %
Parallel Tests
PAR-CUL-N
17205
47.34 %
42079
45.23 %
PAR-CUL-P
798
2.20 %
xx-xxxx-xxxx
1.82 %
PAR-HERD-S
40
0.11 %
1040
1.12 %
PAR-IR-EX
42
0.12 %
92
0.10 %
PAR-IR-SEV
154
0.42 %
345
0.37 %
PAR-NEW-IN
8874
24.42 %
31375
33.72 %
PAR-OTHER
1503
4.14 %
3772
4.05 %
PAR-PERSIS
5817
16.01 %
9454
10.16 %
PAR-RAPID
48
0.13 %
334
0.36 %
PAR-RECUR
987
2.72 %
1411
1.52 %
Total(% of all samples)
35468
97.59 %
91600
98.45 %
Serial/Extended Tests
SER-ANOM
12
0.03 %
12
0.01 %
SER-FLEXI
860
2.37 %
1375
1.48 %
SER-FRAUD
0
0.00 %
SER-NSR
4
0.01 %
4
0.00 %
SER-OTHER
0
0.00 %
52
0.06 %
Total(% of all samples)
876
2.41 %
1443
1.55 %
Total
36344
100.00 %
93043
100.00 %
Retests
Total (% of all samples)
2328
6.41 %
6605
7.10 %
England
1829
5.03 %
5246
5.64 %
Wales
499
1.37 %
1318
1.42 %
Scotland
41
0.04 %
Resamples
Total (% of all samples)
2022
5.56 %
5799
6.23 %
England
1621
4.46 %
4667
5.02 %
Wales
401
1.10 %
1093
1.17 %
Scotland
39
0.04 %
Rejects
Total (% of all samples)
110
0.30 %
339
0.36 %
England
100
0.28 %
271
0.29 %
Wales
9
0.02 %
60
0.06 %
Scotland
1
0.00 %
8
0.01 %
Table 1. Monthly Totals for March 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-ANOM
SER-FLEXI
SER-NSR
Breakdown of
Reason for
submission
PAR-CUL-N
17205
47.3 %
PAR-CUL-P
798
2.2 %
PAR-HERD-S
40
0.1 %
PAR-IR-EX
42
0.1 %
PAR-IR-SEV
154
0.4 %
PAR-NEW-IN
8874
24.4 %
PAR-OTHER
1503
4.1 %
PAR-PERSIS
5817
16.0 %
PAR-RAPID
48
0.1 %
PAR-RECUR
987
2.7 %
SER-ANOM
12
0.0 %
SER-FLEXI
860
2.4 %
SER-NSR
4
0.0 %
Total
36344
100.00 %
Figure 1. Number of samples submitted in each category during March 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
Argyll & Bute
W Glamorgan
S Wales
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Northants
Lincs
Leics & Rut
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during March 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
2000
4000
6000
8000
10000
12000
14000
16000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
2
505
1.39 %
36
7.13 %
456
90.30 %
16
3.17 %
11
2.18 %
2
0.40 %
PAR-PERSIS
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
SER-ANOM
1
12
0.03 %
0
0.00 %
9
75.00 %
2
16.67 %
2
16.67 %
1
8.33 %
Corn & Scilly
PAR-CUL-N
19
1,565
4.31 %
81
5.18 %
1354
86.52 %
141
9.01 %
129
8.24 %
1
0.06 %
PAR-PERSIS
2
569
1.57 %
29
5.10 %
519
91.21 %
23
4.04 %
21
3.69 %
0
0.00 %
Devon
PAR-CUL-N
60
4,082
11.23 %
291
7.13 %
3541
86.75 %
284
6.96 %
242
5.93 %
8
0.20 %
PAR-CUL-P
3
150
0.41 %
7
4.67 %
135
90.00 %
10
6.67 %
8
5.33 %
0
0.00 %
PAR-NEW-IN
1
5
0.01 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-PERSIS
1
17
0.05 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
Dorset
PAR-CUL-N
10
746
2.05 %
33
4.42 %
670
89.81 %
51
6.84 %
42
5.63 %
1
0.13 %
Gloucs
PAR-CUL-N
11
1,182
3.25 %
68
5.75 %
1055
89.26 %
63
5.33 %
55
4.65 %
4
0.34 %
Heref
PAR-CUL-N
2
222
0.61 %
5
2.25 %
205
92.34 %
14
6.31 %
12
5.41 %
0
0.00 %
Shropshire
PAR-CUL-N
6
602
1.66 %
30
4.98 %
545
90.53 %
32
5.32 %
27
4.49 %
0
0.00 %
PAR-OTHER
2
508
1.40 %
20
3.94 %
474
93.31 %
16
3.15 %
7
1.38 %
7
1.38 %
Somerset excl N
PAR-CUL-N
14
1,617
4.45 %
165
10.20 %
1387
85.78 %
77
4.76 %
59
3.65 %
6
0.37 %
Staffs
PAR-CUL-N
15
3,367
9.26 %
99
2.94 %
3059
90.85 %
231
6.86 %
208
6.18 %
1
0.03 %
Wiltshire
PAR-CUL-N
5
572
1.57 %
56
9.79 %
478
83.57 %
39
6.82 %
36
6.29 %
2
0.35 %
PAR-CUL-P
2
351
0.97 %
15
4.27 %
314
89.46 %
15
4.27 %
9
2.56 %
13
3.70 %
PAR-PERSIS
1
184
0.51 %
14
7.61 %
166
90.22 %
4
2.17 %
4
2.17 %
0
0.00 %
England - Edge
Berks
PAR-CUL-P
1
297
0.82 %
3
1.01 %
271
91.25 %
9
3.03 %
5
1.68 %
18
6.06 %
PAR-NEW-IN
2
10
0.03 %
1
10.00 %
9
90.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.05 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
5
979
2.69 %
17
1.74 %
923
94.28 %
44
4.49 %
39
3.98 %
0
0.00 %
Cheshire
PAR-CUL-N
12
1,872
5.15 %
68
3.63 %
1717
91.72 %
100
5.34 %
87
4.65 %
0
0.00 %
PAR-NEW-IN
2
269
0.74 %
10
3.72 %
249
92.57 %
13
4.83 %
10
3.72 %
0
0.00 %
Derbyshire
PAR-CUL-N
1
147
0.40 %
9
6.12 %
73
49.66 %
66
44.90 %
65
44.22 %
0
0.00 %
Table 2a. Summary by County for March 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
21
1,868
5.14 %
78
4.18 %
1574
84.26 %
222
11.88 %
216
11.56 %
0
0.00 %
E Sussex
PAR-CUL-N
4
709
1.95 %
74
10.44 %
586
82.65 %
38
5.36 %
34
4.80 %
15
2.12 %
PAR-NEW-IN
2
37
0.10 %
5
13.51 %
24
64.86 %
9
24.32 %
7
18.92 %
1
2.70 %
Hampshire
PAR-NEW-IN
2
167
0.46 %
4
2.40 %
153
91.62 %
11
6.59 %
10
5.99 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
4
631
1.74 %
26
4.12 %
554
87.80 %
53
8.40 %
51
8.08 %
0
0.00 %
Northants
PAR-NEW-IN
4
354
0.97 %
12
3.39 %
326
92.09 %
17
4.80 %
16
4.52 %
0
0.00 %
Oxon
PAR-NEW-IN
7
75
0.21 %
1
1.33 %
68
90.67 %
7
9.33 %
6
8.00 %
0
0.00 %
PAR-PERSIS
1
84
0.23 %
4
4.76 %
79
94.05 %
1
1.19 %
1
1.19 %
0
0.00 %
Warks
PAR-NEW-IN
10
1,019
2.80 %
40
3.93 %
931
91.36 %
52
5.10 %
48
4.71 %
0
0.00 %
PAR-PERSIS
2
29
0.08 %
3
10.34 %
18
62.07 %
8
27.59 %
8
27.59 %
0
0.00 %
England - LRA
Cleveland
PAR-NEW-IN
1
8
0.02 %
0
0.00 %
5
62.50 %
3
37.50 %
3
37.50 %
0
0.00 %
Cumbria
PAR-NEW-IN
4
787
2.17 %
25
3.18 %
711
90.34 %
44
5.59 %
41
5.21 %
10
1.27 %
Gr Manchester
PAR-NEW-IN
2
338
0.93 %
12
3.55 %
314
92.90 %
13
3.85 %
12
3.55 %
0
0.00 %
Herts
PAR-NEW-IN
2
15
0.04 %
0
0.00 %
13
86.67 %
0
0.00 %
0
0.00 %
2
13.33 %
Lincs
PAR-NEW-IN
5
604
1.66 %
20
3.31 %
538
89.07 %
45
7.45 %
41
6.79 %
5
0.83 %
N Yorks
PAR-NEW-IN
1
7
0.02 %
1
14.29 %
6
85.71 %
1
14.29 %
0
0.00 %
0
0.00 %
Suffolk
PAR-NEW-IN
3
327
0.90 %
13
3.98 %
272
83.18 %
43
13.15 %
39
11.93 %
3
0.92 %
S Yorks
PAR-NEW-IN
2
30
0.08 %
3
10.00 %
22
73.33 %
7
23.33 %
5
16.67 %
0
0.00 %
England
259
26938
74.12 %
1380
5.12 %
23837
88.49 %
1829
6.79 %
1621
6.02 %
100
0.37 %
Wales - High TB
Carms
PAR-IR-EX
4
10
0.03 %
1
10.00 %
6
60.00 %
2
20.00 %
2
20.00 %
1
10.00 %
PAR-IR-SEV
1
10
0.03 %
3
30.00 %
7
70.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
7
658
1.81 %
38
5.78 %
594
90.27 %
30
4.56 %
26
3.95 %
0
0.00 %
PAR-RAPID
4
8
0.02 %
1
12.50 %
7
87.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
211
0.58 %
7
3.32 %
195
92.42 %
10
4.74 %
8
3.79 %
1
0.47 %
SER-FLEXI
2
36
0.10 %
3
8.33 %
33
91.67 %
2
5.56 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-IR-SEV
4
19
0.05 %
4
21.05 %
14
73.68 %
1
5.26 %
1
5.26 %
0
0.00 %
PAR-PERSIS
2
279
0.77 %
12
4.30 %
263
94.27 %
6
2.15 %
4
1.43 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gwent
PAR-IR-SEV
1
10
0.03 %
0
0.00 %
10
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
122
0.34 %
6
4.92 %
112
91.80 %
4
3.28 %
4
3.28 %
0
0.00 %
PAR-RAPID
1
9
0.02 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
2
16
0.04 %
5
31.25 %
11
68.75 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
8
79
0.22 %
15
18.99 %
61
77.22 %
3
3.80 %
3
3.80 %
0
0.00 %
PAR-OTHER
4
436
1.20 %
34
7.80 %
391
89.68 %
14
3.21 %
11
2.52 %
0
0.00 %
PAR-PERSIS
23
3,380
9.30 %
84
2.49 %
3190
94.38 %
139
4.11 %
103
3.05 %
3
0.09 %
PAR-RAPID
6
11
0.03 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
7
669
1.84 %
25
3.74 %
601
89.84 %
46
6.88 %
41
6.13 %
2
0.30 %
SER-FLEXI
14
253
0.70 %
24
9.49 %
226
89.33 %
2
0.79 %
2
0.79 %
1
0.40 %
Powys
PAR-IR-EX
3
12
0.03 %
3
25.00 %
8
66.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-IR-SEV
6
19
0.05 %
3
15.79 %
15
78.95 %
1
5.26 %
1
5.26 %
0
0.00 %
PAR-OTHER
6
357
0.98 %
23
6.44 %
309
86.55 %
30
8.40 %
25
7.00 %
0
0.00 %
PAR-PERSIS
1
10
0.03 %
0
0.00 %
9
90.00 %
1
10.00 %
1
10.00 %
0
0.00 %
PAR-RAPID
4
7
0.02 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
6
0.02 %
2
33.33 %
4
66.67 %
1
16.67 %
0
0.00 %
0
0.00 %
S Wales
PAR-HERD-S
3
38
0.10 %
1
2.63 %
35
92.11 %
2
5.26 %
2
5.26 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
67
0.18 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
246
0.68 %
17
6.91 %
221
89.84 %
11
4.47 %
8
3.25 %
0
0.00 %
Ceredigion
PAR-OTHER
2
5
0.01 %
0
0.00 %
3
60.00 %
2
40.00 %
2
40.00 %
0
0.00 %
PAR-PERSIS
1
72
0.20 %
5
6.94 %
66
91.67 %
3
4.17 %
1
1.39 %
0
0.00 %
Gwynedd
PAR-NEW-IN
3
467
1.28 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
1
4
0.01 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
17
0.05 %
2
11.76 %
13
76.47 %
2
11.76 %
2
11.76 %
0
0.00 %
PAR-NEW-IN
5
404
1.11 %
38
9.41 %
349
86.39 %
23
5.69 %
17
4.21 %
0
0.00 %
PAR-PERSIS
4
287
0.79 %
17
5.92 %
248
86.41 %
30
10.45 %
22
7.67 %
0
0.00 %
PAR-RAPID
3
12
0.03 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for March 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
104
0.29 %
19
18.27 %
83
79.81 %
2
1.92 %
2
1.92 %
0
0.00 %
SER-FLEXI
1
41
0.11 %
3
7.32 %
38
92.68 %
1
2.44 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
1
27
0.07 %
0
0.00 %
22
81.48 %
5
18.52 %
5
18.52 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
18
0.05 %
5
27.78 %
12
66.67 %
1
5.56 %
1
5.56 %
0
0.00 %
NE Wales
PAR-NEW-IN
3
446
1.23 %
15
3.36 %
412
92.38 %
27
6.05 %
19
4.26 %
0
0.00 %
PAR-OTHER
2
6
0.02 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
SER-FLEXI
3
506
1.39 %
46
9.09 %
426
84.19 %
39
7.71 %
34
6.72 %
0
0.00 %
Wales
159
9402
25.87 %
487
5.18 %
8505
90.46 %
499
5.31 %
401
4.27 %
9
0.10 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
4
0.01 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Scotland
1
4
0.01 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Grand Total
Sum:
419
36344
1867
32345
2328
2022
110
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
2
505
0.54 %
36
7.13 %
456
90.30 %
16
3.17 %
11
2.18 %
2
0.40 %
PAR-PERSIS
7
721
0.77 %
131
18.17 %
510
70.74 %
80
11.10 %
71
9.85 %
9
1.25 %
SER-ANOM
1
12
0.01 %
0
0.00 %
9
75.00 %
2
16.67 %
2
16.67 %
1
8.33 %
Corn & Scilly
PAR-CUL-N
42
3,965
4.26 %
193
4.87 %
3341
84.26 %
464
11.70 %
420
10.59 %
11
0.28 %
PAR-CUL-P
5
868
0.93 %
34
3.92 %
767
88.36 %
70
8.06 %
65
7.49 %
2
0.23 %
PAR-PERSIS
2
569
0.61 %
29
5.10 %
519
91.21 %
23
4.04 %
21
3.69 %
0
0.00 %
Devon
PAR-CUL-N
133
13,581
14.60 %
970
7.14 %
11815
87.00 %
827
6.09 %
738
5.43 %
58
0.43 %
PAR-CUL-P
4
156
0.17 %
7
4.49 %
140
89.74 %
11
7.05 %
9
5.77 %
0
0.00 %
PAR-NEW-IN
7
596
0.64 %
26
4.36 %
536
89.93 %
33
5.54 %
31
5.20 %
3
0.50 %
PAR-PERSIS
1
17
0.02 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
Dorset
PAR-CUL-N
29
2,348
2.52 %
166
7.07 %
2057
87.61 %
142
6.05 %
122
5.20 %
3
0.13 %
Gloucs
PAR-CUL-N
28
2,662
2.86 %
160
6.01 %
2295
86.21 %
204
7.66 %
179
6.72 %
28
1.05 %
Heref
PAR-CUL-N
8
584
0.63 %
44
7.53 %
478
81.85 %
45
7.71 %
43
7.36 %
19
3.25 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
110
0.12 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
SER-OTHER
1
52
0.06 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
19
3,709
3.99 %
140
3.77 %
3364
90.70 %
223
6.01 %
201
5.42 %
4
0.11 %
PAR-OTHER
2
508
0.55 %
20
3.94 %
474
93.31 %
16
3.15 %
7
1.38 %
7
1.38 %
Somerset excl N
PAR-CUL-N
31
2,806
3.02 %
241
8.59 %
2462
87.74 %
135
4.81 %
97
3.46 %
6
0.21 %
PAR-NEW-IN
2
370
0.40 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.50 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
Staffs
PAR-CUL-N
32
5,603
6.02 %
218
3.89 %
4992
89.10 %
431
7.69 %
391
6.98 %
2
0.04 %
PAR-CUL-P
3
26
0.03 %
2
7.69 %
18
69.23 %
6
23.08 %
6
23.08 %
0
0.00 %
Wiltshire
PAR-CUL-N
20
1,981
2.13 %
170
8.58 %
xx-xxxx-xxxx
84.86 %
147
7.42 %
124
6.26 %
6
0.30 %
PAR-CUL-P
2
351
0.38 %
15
4.27 %
314
89.46 %
15
4.27 %
9
2.56 %
13
3.70 %
PAR-NEW-IN
2
191
0.21 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
207
0.22 %
17
8.21 %
180
86.96 %
11
5.31 %
10
4.83 %
0
0.00 %
Worcs
PAR-CUL-N
2
128
0.14 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
England - Edge
Berks
PAR-CUL-P
1
297
0.32 %
3
1.01 %
271
91.25 %
9
3.03 %
5
1.68 %
18
6.06 %
PAR-NEW-IN
7
356
0.38 %
22
6.18 %
305
85.67 %
33
9.27 %
29
8.15 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.02 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
7
1,059
1.14 %
21
1.98 %
997
94.15 %
46
4.34 %
41
3.87 %
0
0.00 %
Cheshire
PAR-CUL-N
19
2,309
2.48 %
82
3.55 %
2124
91.99 %
117
5.07 %
102
4.42 %
1
0.04 %
PAR-NEW-IN
14
1,666
1.79 %
66
3.96 %
1529
91.78 %
92
5.52 %
71
4.26 %
0
0.00 %
PAR-PERSIS
2
14
0.02 %
1
7.14 %
9
64.29 %
3
21.43 %
3
21.43 %
1
7.14 %
Derbyshire
PAR-CUL-N
4
937
1.01 %
50
5.34 %
775
82.71 %
116
12.38 %
112
11.95 %
0
0.00 %
PAR-NEW-IN
49
6,027
6.48 %
272
4.51 %
5119
84.93 %
690
11.45 %
619
10.27 %
17
0.28 %
E Sussex
PAR-CUL-N
4
709
0.76 %
74
10.44 %
586
82.65 %
38
5.36 %
34
4.80 %
15
2.12 %
PAR-NEW-IN
3
55
0.06 %
8
14.55 %
35
63.64 %
14
25.45 %
11
20.00 %
1
1.82 %
Hampshire
PAR-CUL-N
2
217
0.23 %
14
6.45 %
199
91.71 %
5
2.30 %
4
1.84 %
0
0.00 %
PAR-NEW-IN
10
1,759
1.89 %
58
3.30 %
1568
89.14 %
143
8.13 %
131
7.45 %
2
0.11 %
PAR-PERSIS
1
76
0.08 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
18
2,278
2.45 %
106
4.65 %
2039
89.51 %
154
6.76 %
133
5.84 %
0
0.00 %
Northants
PAR-NEW-IN
17
1,181
1.27 %
44
3.73 %
1064
90.09 %
80
6.77 %
73
6.18 %
0
0.00 %
PAR-PERSIS
1
168
0.18 %
2
1.19 %
156
92.86 %
11
6.55 %
10
5.95 %
0
0.00 %
Notts
PAR-NEW-IN
1
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Oxon
PAR-NEW-IN
30
2,071
2.23 %
75
3.62 %
1860
89.81 %
147
7.10 %
132
6.37 %
4
0.19 %
PAR-PERSIS
2
164
0.18 %
8
4.88 %
150
91.46 %
6
3.66 %
6
3.66 %
0
0.00 %
Warks
PAR-NEW-IN
32
2,739
2.94 %
111
4.05 %
2459
89.78 %
176
6.43 %
159
5.81 %
10
0.37 %
PAR-PERSIS
2
29
0.03 %
3
10.34 %
18
62.07 %
8
27.59 %
8
27.59 %
0
0.00 %
England - LRA
Cleveland
PAR-NEW-IN
2
194
0.21 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.02 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
12
2,127
2.29 %
67
3.15 %
1957
92.01 %
114
5.36 %
93
4.37 %
10
0.47 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.10 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gr Manchester
PAR-NEW-IN
5
380
0.41 %
14
3.68 %
352
92.63 %
16
4.21 %
14
3.68 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.12 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Lancashire
PAR-NEW-IN
2
42
0.05 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Lincs
PAR-NEW-IN
11
1,130
1.21 %
41
3.63 %
1011
89.47 %
81
7.17 %
72
6.37 %
6
0.53 %
N Yorks
PAR-NEW-IN
10
420
0.45 %
27
6.43 %
353
84.05 %
45
10.71 %
39
9.29 %
1
0.24 %
Suffolk
PAR-NEW-IN
3
327
0.35 %
13
3.98 %
272
83.18 %
43
13.15 %
39
11.93 %
3
0.92 %
S Yorks
PAR-NEW-IN
3
206
0.22 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
673
72279
77.68 %
3939
5.45 %
63402
87.72 %
5246
7.26 %
4667
6.46 %
271
0.37 %
Wales - High TB
Carms
PAR-IR-EX
4
10
0.01 %
1
10.00 %
6
60.00 %
2
20.00 %
2
20.00 %
1
10.00 %
PAR-IR-SEV
6
40
0.04 %
13
32.50 %
24
60.00 %
4
10.00 %
3
7.50 %
0
0.00 %
PAR-NEW-IN
1
6
0.01 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
10
274
0.29 %
24
8.76 %
231
84.31 %
19
6.93 %
17
6.20 %
2
0.73 %
PAR-PERSIS
14
797
0.86 %
54
6.78 %
712
89.34 %
42
5.27 %
31
3.89 %
0
0.00 %
PAR-RAPID
24
42
0.05 %
10
23.81 %
32
76.19 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
211
0.23 %
7
3.32 %
195
92.42 %
10
4.74 %
8
3.79 %
1
0.47 %
SER-FLEXI
3
59
0.06 %
10
xx-xxxx-xxxx %
48
81.36 %
3
5.08 %
1
1.69 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
5
0.01 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
37
0.04 %
11
29.73 %
25
67.57 %
1
2.70 %
1
2.70 %
0
0.00 %
PAR-OTHER
2
39
0.04 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
8
760
0.82 %
49
6.45 %
674
88.68 %
35
4.61 %
24
3.16 %
13
1.71 %
PAR-RAPID
11
34
0.04 %
7
20.59 %
26
76.47 %
2
5.88 %
1
2.94 %
0
0.00 %
PAR-RECUR
1
5
0.01 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
Gwent
PAR-IR-SEV
1
10
0.01 %
0
0.00 %
10
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
155
0.17 %
9
5.81 %
142
91.61 %
7
4.52 %
4
2.58 %
0
0.00 %
PAR-PERSIS
7
98
0.11 %
7
7.14 %
85
86.73 %
5
5.10 %
5
5.10 %
1
1.02 %
PAR-RAPID
2
10
0.01 %
2
20.00 %
8
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
5
29
0.03 %
6
20.69 %
23
79.31 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
18
199
0.21 %
47
23.62 %
145
72.86 %
8
4.02 %
7
3.52 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
25
1,269
1.36 %
77
6.07 %
1137
89.60 %
63
4.96 %
52
4.10 %
3
0.24 %
PAR-PERSIS
51
3,725
4.00 %
127
3.41 %
3476
93.32 %
157
4.21 %
119
3.19 %
3
0.08 %
PAR-RAPID
52
191
0.21 %
50
26.18 %
137
71.73 %
5
2.62 %
4
2.09 %
0
0.00 %
PAR-RECUR
9
999
1.07 %
43
4.30 %
900
90.09 %
60
6.01 %
54
5.41 %
2
0.20 %
SER-FLEXI
18
479
0.51 %
36
7.52 %
435
90.81 %
10
2.09 %
7
1.46 %
1
0.21 %
Powys
PAR-IR-EX
4
44
0.05 %
17
38.64 %
23
52.27 %
5
11.36 %
4
9.09 %
0
0.00 %
PAR-IR-SEV
8
41
0.04 %
5
12.20 %
35
85.37 %
1
2.44 %
1
2.44 %
0
0.00 %
PAR-NEW-IN
1
239
0.26 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
11
731
0.79 %
40
5.47 %
645
88.24 %
52
7.11 %
42
5.75 %
4
0.55 %
PAR-PERSIS
22
908
0.98 %
29
3.19 %
799
88.00 %
77
8.48 %
72
7.93 %
8
0.88 %
PAR-RAPID
8
21
0.02 %
1
4.76 %
20
95.24 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
81
0.09 %
15
18.52 %
60
74.07 %
8
9.88 %
6
7.41 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
1.11 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
W Glamorgan
PAR-OTHER
2
67
0.07 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.15 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.01 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-PERSIS
5
422
0.45 %
32
7.58 %
369
87.44 %
26
6.16 %
21
4.98 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
43
0.05 %
9
20.93 %
33
76.74 %
3
6.98 %
1
2.33 %
0
0.00 %
Ceredigion
PAR-OTHER
3
106
0.11 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
1
72
0.08 %
5
6.94 %
66
91.67 %
3
4.17 %
1
1.39 %
0
0.00 %
SER-FLEXI
3
148
0.16 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.50 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
18
0.02 %
2
11.11 %
14
77.78 %
2
11.11 %
2
11.11 %
0
0.00 %
PAR-NEW-IN
29
2,142
2.30 %
109
5.09 %
1870
87.30 %
180
8.40 %
161
7.52 %
2
0.09 %
PAR-OTHER
2
3
0.00 %
0
0.00 %
1
33.33 %
2
66.67 %
2
66.67 %
0
0.00 %
PAR-PERSIS
7
447
0.48 %
18
4.03 %
402
89.93 %
37
8.28 %
27
6.04 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
4
31
0.03 %
6
19.35 %
24
77.42 %
1
3.23 %
1
3.23 %
0
0.00 %
PAR-RECUR
1
104
0.11 %
19
18.27 %
83
79.81 %
2
1.92 %
2
1.92 %
0
0.00 %
SER-FLEXI
1
41
0.04 %
3
7.32 %
38
92.68 %
1
2.44 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
5
417
0.45 %
12
2.88 %
357
85.61 %
54
12.95 %
47
11.27 %
1
0.24 %
PAR-PERSIS
1
14
0.02 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
92
0.10 %
6
6.52 %
81
88.04 %
7
7.61 %
4
4.35 %
1
1.09 %
S Glamorgan
PAR-RAPID
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
126
0.14 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
300
0.32 %
12
4.00 %
257
85.67 %
40
13.33 %
31
10.33 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
18
0.02 %
5
27.78 %
12
66.67 %
1
5.56 %
1
5.56 %
0
0.00 %
NE Wales
PAR-NEW-IN
17
1,870
2.01 %
99
5.29 %
1630
87.17 %
170
9.09 %
138
7.38 %
3
0.16 %
PAR-OTHER
4
152
0.16 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
SER-FLEXI
3
506
0.54 %
46
9.09 %
426
84.19 %
39
7.71 %
34
6.72 %
0
0.00 %
Wales
467
20339
21.86 %
1183
5.82 %
18003
88.51 %
1318
6.48 %
1093
5.37 %
60
0.29 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
3
401
0.43 %
34
8.48 %
323
80.55 %
39
9.73 %
37
9.23 %
7
1.75 %
Dumfries & G
PAR-NEW-IN
1
8
0.01 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.01 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
6
425
0.46 %
35
8.24 %
343
80.71 %
41
9.65 %
39
9.18 %
8
1.88 %
Grand Total
Sum:
1146
93043
5157
81748
6605
5799
339
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol March 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
258
26926
100.0%
1380
5.1%
23828
88.5%
1827
6.8%
1619
6.0%
99
0.4%
Serial/Extended
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7%
2
16.7%
1
8.3%
Total
259
26938
100.0%
1380
5.1%
23837
88.5%
1829
6.8%
1621
6.0%
100
0.4%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
1
4
100.0%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
1
4
100.0%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Wales
Parallel
137
8542
90.9%
404
4.7%
7766
90.9%
453
5.3%
364
4.3%
8
0.1%
Serial/Extended
22
860
9.1%
83
9.7%
739
85.9%
46
5.3%
37
4.3%
1
0.1%
Total
159
9402
100.0%
487
5.2%
8505
90.5%
499
5.3%
401
4.3%
9
0.1%
GB
Parallel
395
35468
97.6%
1784
5.0%
31594
89.1%
2280
6.4%
1983
5.6%
107
0.3%
Serial/Extended
24
876
2.4%
83
9.5%
751
85.7%
48
5.5%
39
4.5%
3
0.3%
Total
419
36344
100.0%
1867
5.1%
32345
89.0%
2328
6.4%
2022
5.6%
110
0.3%
259.00
159.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
671
72215
99.9%
3939
5.5%
63342
87.7%
5243
7.3%
4664
6.5%
270
0.4%
Serial/Extended
2
64
0.1%
0
0.0 %
60
93.8%
3
4.7%
3
4.7%
1
1.6%
Total
673
72279
100.0%
3939
5.4%
63402
87.7%
5246
7.3%
4667
6.5%
271
0.4%
Scotland
Parallel
5
421
99.1%
35
8.3%
340
80.8%
41
9.7%
39
9.3%
7
1.7%
Serial/Extended
1
4
0.9%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
6
425
100.0%
35
8.2%
343
80.7%
41
9.6%
39
9.2%
8
1.9%
Wales
Parallel
434
18964
93.2%
1053
5.6%
xx-xxxx-xxxx
88.7%
1250
6.6%
1041
5.5%
58
0.3%
Serial/Extended
33
1375
6.8%
130
9.5%
1191
86.6%
68
4.9%
52
3.8%
2
0.1%
Total
467
20339
100.0%
1183
5.8%
18003
88.5%
1318
6.5%
1093
5.4%
60
0.3%
GB
Parallel
1110
91600
98.4%
5027
5.5%
80494
87.9%
6534
7.1%
5744
6.3%
335
0.4%
Serial/Extended
36
1443
1.6%
130
9.0%
1254
86.9%
71
4.9%
55
3.8%
4
0.3%
Total
1146
93043
100.0%
5157
5.5%
81748
87.9%
6605
7.1%
5799
6.2%
339
0.4%
673.00
467.00
6.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Extended Flexible IFN-GAMMA test
22
860
2.4%
83
9.7%
739
85.9%
46
5.3 %
37
4.3 %
1
0.1 %
Parallel - Herd or Group Slaughter
5
40
0.1%
1
2.5%
37
92.5%
2
5.0 %
2
5.0 %
0
Parallel New Infection
92
8874
24.4%
336
3.8%
7885
88.9%
689
7.8 %
631
7.1 %
22
0.2 %
Parallel - new infection cull area
162
17205
47.3%
1016
5.9%
15141
88.0%
1153
6.7 %
1008
5.9 %
40
0.2 %
Parallel - Other
20
1503
4.1%
83
5.5%
1356
90.2%
75
5.0 %
57
3.8 %
7
0.5 %
Parallel Persistent Infection
48
5817
16.0%
224
3.9%
5389
92.6%
258
4.4 %
201
3.5 %
3
0.1 %
Parallel - persistent infection cull area
6
798
2.2%
25
3.1%
720
90.2%
34
4.3 %
22
2.8 %
31
3.9 %
Parallel - Rapid Testing Two-times IRs
19
48
0.1%
9
18.8%
39
81.3%
0
0
0
PAR-IR-EX
10
42
0.1%
12
28.6%
26
61.9%
3
7.1 %
3
7.1 %
1
2.4 %
PAR-IR-SEV
22
154
0.4%
27
17.5%
120
77.9%
7
4.5 %
7
4.5 %
0
PAR-RECUR
11
987
2.7%
51
5.2%
881
89.3%
59
6.0 %
52
5.3 %
3
0.3 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
419
36344
100.0%
1867
5.1%
32345
89.0%
2328
6.4 %
2022
5.6 %
110
0.3 %
Table 4a. Summaryof IFN Gamma results by submission reason (March 2021)
England - HRA
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Parallel New Infection
1
5
0.0%
0
0.0 %
3
60.0%
2
40.0 %
2
40.0 %
0
Parallel - new infection cull area
144
14460
39.8%
864
6.0%
12750
88.2%
948
6.6 %
821
5.7 %
25
0.2 %
Parallel - Other
2
508
1.4%
20
3.9%
474
93.3%
16
3.1 %
7
1.4 %
7
1.4 %
Parallel Persistent Infection
5
772
2.1%
44
5.7%
701
90.8%
29
3.8 %
27
3.5 %
0
Parallel - persistent infection cull area
5
501
1.4%
22
4.4%
449
89.6%
25
5.0 %
17
3.4 %
13
2.6 %
England - HRA
158
16258
44.7%
950
5.8%
14386
88.5%
1022
6.3 %
876
5.4 %
46
0.3 %
England - Edge
Parallel New Infection
59
5409
14.9%
194
3.6%
4811
88.9%
428
7.9 %
403
7.5 %
1
0.0 %
Parallel - new infection cull area
18
2745
7.6%
152
5.5%
2391
87.1%
205
7.5 %
187
6.8 %
15
0.5 %
Parallel Persistent Infection
3
113
0.3%
7
6.2%
97
85.8%
9
8.0 %
9
8.0 %
0
Parallel - persistent infection cull area
1
297
0.8%
3
1.0%
271
91.2%
9
3.0 %
5
1.7 %
18
6.1 %
England - Edge
81
8564
23.6%
356
4.2%
7570
88.4%
651
7.6 %
604
7.1 %
34
0.4 %
England - LRA
Parallel New Infection
20
2116
5.8%
74
3.5%
1881
88.9%
156
7.4 %
141
6.7 %
20
0.9 %
England - LRA
20
2116
5.8%
74
3.5%
1881
88.9%
156
7.4 %
141
6.7 %
20
0.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
17
295
0.8%
29
9.8%
263
89.2%
5
1.7 %
2
0.7 %
1
0.3 %
Parallel - Herd or Group Slaughter
3
38
0.1%
1
2.6%
35
92.1%
2
5.3 %
2
5.3 %
0
Parallel - Other
14
984
2.7%
63
6.4%
875
88.9%
55
5.6 %
46
4.7 %
0
Parallel Persistent Infection
33
4327
11.9%
134
3.1%
4056
93.7%
176
4.1 %
134
3.1 %
3
0.1 %
Parallel - Rapid Testing Two-times IRs
15
35
0.1%
6
17.1%
29
82.9%
0
0
0
PAR-IR-EX
9
38
0.1%
9
23.7%
25
65.8%
3
7.9 %
3
7.9 %
1
2.6 %
PAR-IR-SEV
20
137
0.4%
25
18.2%
107
78.1%
5
3.6 %
5
3.6 %
0
PAR-RECUR
9
880
2.4%
32
3.6%
796
90.5%
56
6.4 %
49
5.6 %
3
0.3 %
Wales - High TB
120
6734
18.5%
299
4.4%
6186
91.9%
302
4.5 %
241
3.6 %
8
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
41
0.1%
3
7.3%
38
92.7%
1
2.4 %
0
0
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
9
898
2.5%
53
5.9%
778
86.6%
76
8.5 %
66
7.3 %
1
0.1 %
Parallel - Other
2
5
0.0%
0
0.0 %
3
60.0%
2
40.0 %
2
40.0 %
0
Parallel Persistent Infection
7
605
1.7%
39
6.4%
535
88.4%
44
7.3 %
31
5.1 %
0
Parallel - Rapid Testing Two-times IRs
4
13
0.0%
3
23.1%
10
76.9%
0
0
0
PAR-IR-EX
1
4
0.0%
3
75.0%
1
25.0%
0
0
0
PAR-IR-SEV
2
17
0.0%
2
11.8%
13
76.5%
2
11.8 %
2
11.8 %
0
PAR-RECUR
2
107
0.3%
19
17.8%
85
79.4%
3
2.8 %
3
2.8 %
0
Wales - Intermediate TB
29
xx-xxxx-xxxx
4.7%
122
7.2%
1464
86.6%
128
7.6 %
104
6.2 %
1
0.1 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
524
1.4%
51
9.7%
438
83.6%
40
7.6 %
35
6.7 %
0
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
3
446
1.2%
15
3.4%
412
92.4%
27
6.1 %
19
4.3 %
0
Parallel - Other
2
6
0.0%
0
0.0 %
4
66.7%
2
33.3 %
2
33.3 %
0
Wales - Low TB
10
977
2.7%
66
6.8%
855
87.5%
69
7.1 %
56
5.7 %
0
Scotland - Scotland
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland - Scotland
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Extended Flexible IFN-GAMMA test
33
1375
1.5%
130
9.5%
1191
86.6%
68
4.9 %
52
3.8 %
2
0.1 %
Parallel - Herd or Group Slaughter
8
1040
1.1%
45
4.3%
952
91.5%
44
4.2 %
38
3.7 %
5
0.5 %
Parallel New Infection
326
31375
33.7%
1358
4.3%
27674
88.2%
2563
8.2 %
2263
7.2 %
80
0.3 %
Parallel - new infection cull area
377
42079
45.2%
2571
6.1%
36758
87.4%
2924
6.9 %
2591
6.2 %
159
0.4 %
Parallel - Other
67
3772
4.1%
205
5.4%
3345
88.7%
225
6.0 %
204
5.4 %
18
0.5 %
Parallel Persistent Infection
140
9454
10.2%
527
5.6%
8433
89.2%
556
5.9 %
459
4.9 %
35
0.4 %
Parallel - persistent infection cull area
15
xx-xxxx-xxxx
1.8%
61
3.6%
1510
88.9%
111
6.5 %
94
5.5 %
33
1.9 %
Parallel - Rapid Testing Two-times IRs
105
334
0.4%
79
23.7%
249
74.6%
8
2.4 %
6
1.8 %
0
PAR-IR-EX
15
92
0.1%
27
29.3%
58
63.0%
7
7.6 %
6
6.5 %
1
1.1 %
PAR-IR-SEV
42
345
0.4%
78
22.6%
253
73.3%
16
4.6 %
14
4.1 %
0
PAR-RECUR
15
1411
1.5%
76
5.4%
1262
89.4%
80
5.7 %
69
4.9 %
4
0.3 %
Serial - Other
1
52
0.1%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
1146
93043
100.0%
5157
5.5%
81748
87.9%
6605
7.1 %
5799
6.2 %
339
0.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Parallel New Infection
11
1157
1.2%
92
8.0%
1005
86.9%
61
5.3 %
57
4.9 %
3
0.3 %
Parallel - new infection cull area
346
37872
40.7%
2350
6.2%
33053
87.3%
2637
7.0 %
2328
6.1 %
141
0.4 %
Parallel - Other
5
976
1.0%
42
4.3%
864
88.5%
51
5.2 %
63
6.5 %
7
0.7 %
Parallel Persistent Infection
14
1624
1.7%
185
11.4%
1311
80.7%
131
8.1 %
119
7.3 %
9
0.6 %
Parallel - persistent infection cull area
14
1401
1.5%
58
4.1%
1239
88.4%
102
7.3 %
89
6.4 %
15
1.1 %
Serial - Other
1
52
0.1%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
188
19203
20.6%
783
4.1%
xx-xxxx-xxxx
88.4%
1578
8.2 %
1402
7.3 %
34
0.2 %
Parallel - new infection cull area
30
4189
4.5%
221
5.3%
3699
88.3%
277
6.6 %
253
6.0 %
16
0.4 %
Parallel Persistent Infection
8
451
0.5%
17
3.8%
402
89.1%
34
7.5 %
31
6.9 %
1
0.2 %
Parallel - persistent infection cull area
1
297
0.3%
3
1.0%
271
91.2%
9
3.0 %
5
1.7 %
18
6.1 %
England - LRA
Parallel New Infection
53
5027
5.4%
188
3.7%
4508
89.7%
353
7.0 %
307
6.1 %
24
0.5 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
673
72279
77.7%
3939
5.4%
63402
87.7%
5246
7.3 %
4667
6.5 %
271
0.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
24
619
0.7%
61
9.9%
543
87.7%
21
3.4 %
14
2.3 %
1
0.2 %
Parallel - Herd or Group Slaughter
5
1030
1.1%
44
4.3%
944
91.7%
43
4.2 %
37
3.6 %
5
0.5 %
Parallel New Infection
2
245
0.3%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
53
2535
2.7%
153
6.0%
2247
88.6%
154
6.1 %
126
5.0 %
9
0.4 %
Parallel Persistent Infection
104
6424
6.9%
268
4.2%
5875
91.5%
321
5.0 %
256
4.0 %
25
0.4 %
Parallel - Rapid Testing Two-times IRs
97
298
0.3%
70
23.5%
223
74.8%
7
2.3 %
5
1.7 %
0
PAR-IR-EX
14
88
0.1%
24
27.3%
57
64.8%
7
8.0 %
6
6.8 %
1
1.1 %
PAR-IR-SEV
39
327
0.4%
76
23.2%
239
73.1%
14
4.3 %
12
3.7 %
0
PAR-RECUR
12
1215
1.3%
51
4.2%
1098
90.4%
71
5.8 %
63
5.2 %
3
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
232
0.2%
18
7.8%
210
90.5%
7
3.0 %
3
1.3 %
1
0.4 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel New Infection
40
3152
3.4%
143
4.5%
2732
86.7%
300
9.5 %
269
8.5 %
8
0.3 %
Parallel - Other
5
109
0.1%
6
5.5%
94
86.2%
12
11.0 %
8
7.3 %
1
0.9 %
Parallel Persistent Infection
14
955
1.0%
57
6.0%
845
88.5%
70
7.3 %
53
5.5 %
0
Parallel - Rapid Testing Two-times IRs
7
35
0.0%
8
22.9%
26
74.3%
1
2.9 %
1
2.9 %
0
PAR-IR-EX
1
4
0.0%
3
75.0%
1
25.0%
0
0
0
PAR-IR-SEV
3
18
0.0%
2
11.1%
14
77.8%
2
11.1 %
2
11.1 %
0
PAR-RECUR
3
196
0.2%
25
12.8%
164
83.7%
9
4.6 %
6
3.1 %
1
0.5 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
524
0.6%
51
9.7%
438
83.6%
40
7.6 %
35
6.7 %
0
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
27
2170
2.3%
111
5.1%
1887
87.0%
210
9.7 %
169
7.8 %
3
0.1 %
Parallel - Other
4
152
0.2%
4
2.6%
140
92.1%
8
5.3 %
7
4.6 %
1
0.7 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales
467
20339
21.9%
1183
5.8%
18003
88.5%
1318
6.5 %
1093
5.4 %
60
0.3 %
Scotland - Scotland
Parallel New Infection
5
421
0.5%
35
8.3%
340
80.8%
41
9.7 %
39
9.3 %
7
1.7 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
6
425
0.5%
35
8.2%
343
80.7%
41
9.6 %
39
9.2 %
8
1.9 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
0
70
0
1
August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
0
65
0
1
October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
3
1
7
0
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
59
0
0
0
0
110
0
0
14
3
1366
3
90
1
4
February
352
28759
235
1637
56
0
0
0
0
96
0
6
9
0
1368
86
7
3
5
March
394
33628
237
1736
499
0
0
0
0
48
0
9
3
0
1110
47
0
0
0
Totals (last
12 months)
3705
292133
2180
13740
6
749
0
0
0
0
1167
15
16
70
3
10833
137
699
6
12
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1981
226468
1303
10216
667
0
0
0
0
1039
8
11
25
2
8386
8
59
0
5
Scotland
48
4504
25
130
0
0
0
0
0
5
1
1
10
0
43
24
42
3
0
Wales
1676
61161
852
3394
82
0
0
0
0
123
6
4
35
1
2404
105
598
3
7
Totals
3705
292133
2180
13740
749
0
0
0
0
1167
15
16
70
3
10833
137
699
6
12
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
80
Wiltshire
Warks
S Wales
Staffs
Shropshire
Pembs
N Yorks
NE Wales
Leics & Rut
Heref
Gwynedd
Gr Manchester
E Sussex
Devon
Cumbria
Cleveland
Ceredigion
Bucks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during March 2021 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
Avon
2.70 %
3.66 %
Berks
1.63 %
2.93 %
Bucks
4.02 %
4.52 %
Carms
3.72 %
4.65 %
Ceredigion
2.13 %
3.20 %
Cheshire
4.53 %
5.28 %
Cleveland
37.50 %
37.50 %
Corn & Scilly
7.03 %
7.69 %
Cumbria
5.21 %
5.59 %
Derbyshire
13.95 %
14.29 %
Devon
5.95 %
6.98 %
Dorset
5.63 %
6.84 %
E Sussex
5.50 %
6.30 %
Gloucs
4.65 %
5.33 %
Gr Manchester
3.55 %
3.85 %
Gwent
2.84 %
2.84 %
Gwynedd
9.26 %
10.08 %
Hampshire
5.99 %
6.59 %
Heref
5.41 %
6.31 %
Herts
Leics & Rut
8.08 %
8.40 %
Lincs
6.79 %
7.45 %
NE Wales
5.36 %
6.90 %
Northants
4.52 %
4.80 %
N Yorks
14.29 %
Oxon
4.40 %
5.03 %
Pembs
3.30 %
4.21 %
Powys
7.69 %
9.05 %
Shropshire
3.06 %
4.32 %
Somerset excl
3.65 %
4.76 %
Staffs
6.18 %
6.86 %
Suffolk
11.93 %
13.15 %
S Wales
5.26 %
5.26 %
S Yorks
16.67 %
23.33 %

Warks
5.34 %
5.73 %
W Glamorgan
8.96 %
10.45 %
Wiltshire
4.43 %
5.24 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
26397
1380
5.2%
26938
1380
5.1%
Wales
9201
487
5.3%
9402
487
5.2%
Scotland
4
0
0.0 %
4
0.0 %
GB
35602
1867
5.2%
36344
1867
5.1%
Table 6a (i): Number of animals and submissions tested in March 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
69243
3939
5.7%
72279
3939
5.4%
Wales
19496
1183
6.1%
20339
1183
5.8%
Scotland
393
35
8.9%
425
35
8.2%
GB
89129
5157
5.8%
93043
5157
5.5%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of March
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
13849
533
3.8%
14090
533
3.8%
Wales
3282
189
5.8%
3363
189
5.6%
Scotland
751
42
5.6%
768
42
5.5%
GB
17882
764
4.3%
18221
764
4.2%
Table 6a (ii): Number of animals and submissions tested in March 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of March
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
62705
2515
4.0%
65835
2515
3.8%
Wales
13942
838
6.0%
14606
839
5.7%
Scotland
2072
66
3.2%
2200
66
3.0%
GB
78719
3419
4.3%
82641
3420
4.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
Devon
PRV-SE
1
62
1
1.61 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
1
0
0
Somerset excl North
PRV-SE
1
1
0
0
Total
5
70
4
5.71 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples March 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
1
1
0
0
Devon
PRV-SE
1
62
1
1.61 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
1
1
0
0
Wiltshire
PRV-SE
1
10
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
11
90
4
4.44 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
3
23
3
13.0%
BOV-IR-EX
1
3
0
0
BOV-OTHER
1
12
0
0
BOV-P
1
26
2
7.7%
OTHER
1
49
2
4.1%
Ceredigion
BOV-IR-C
5
46
7
15.2%
BOV-OTHER
1
73
4
5.5%
North East Wales
BOV-IR-C
1
17
0
0
BOV-IR-EX
1
4
1
25.0%
BOV-P
3
519
9
1.7%
Pembrokeshire
BOV-IR-C
9
92
14
15.2%
BOV-IR-EX
2
16
4
25.0%
BOV-OTHER
6
216
8
3.7%
BOV-P
3
94
11
11.7%
OTHER
1
17
1
5.9%
Powys
BOV-IR-C
6
27
6
22.2%
BOV-P
1
35
1
2.9%
South Wales
BOV-IR-C
1
10
0
0
Wales
47
1,279
73
5.7%
All GB
47
1,279
73
5.7 %
Table 8a Number of Cattle Antibody Tests March 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
Devon
1
95
6
6.3%
England
2
185
6
3.2%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
6
58
13
22.4%
BOV-IR-EX
2
6
2
33.3%
BOV-OTHER
1
12
0
0
BOV-P
1
26
2
7.7%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
7
60
9
15.0%
BOV-OTHER
4
96
13
13.5%
North East Wales
2
24
6
25.0%
BOV-IR-C
1
17
0
0
BOV-IR-EX
1
4
1
25.0%
BOV-P
3
519
9
1.7%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
14
180
29
16.1%
BOV-IR-EX
3
19
5
26.3%
BOV-OTHER
10
434
27
6.2%
BOV-P
4
115
11
9.6%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
OTHER
1
17
1
5.9%
Powys
2
11
1
9.1%
BOV-IR-C
8
34
8
23.5%
BOV-IR-EX
1
32
7
21.9%
BOV-OTHER
2
107
18
16.8%
BOV-P
1
35
1
2.9%
South Wales
4
14
5
35.7%
BOV-IR-C
1
10
0
0
Wales
105
2,141
210
9.8%
All GB
107
2,326
216
9.3 %