Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - April 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 176
Report Period 1st - 30th April 2021
Issued 10 May 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
Parallel blood testing of skin test-negative cattle
in breakdowns herds occurring within 6 months
of lifting restrictions after a previous breakdown
NO (APHA discretion -
Wales only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Test Criteria

Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
April 2021
%
Total 2021
%
Submissions
367
1513
Samples
Total (%)
28108
100.00 %
121151
100.00 %
England
21172
75.32 %
93451
77.14 %
Wales
6622
23.56 %
26961
22.25 %
Scotland
314
1.12 %
739
0.61 %
Parallel Tests
PAR-CUL-N
13475
47.94 %
55554
45.86 %
PAR-CUL-P
1876
6.67 %
3574
2.95 %
PAR-HERD-S
0
0.00 %
1040
0.86 %
PAR-IR-EX
25
0.09 %
117
0.10 %
PAR-IR-SEV
231
0.82 %
576
0.48 %
PAR-NEW-IN
6257
22.26 %
37632
31.06 %
PAR-OTHER
499
1.78 %
4271
3.53 %
PAR-PERSIS
2521
8.97 %
11975
9.88 %
PAR-RAPID
87
0.31 %
421
0.35 %
PAR-RECUR
2584
9.19 %
3995
3.30 %
Total(% of all samples)
27555
98.03 %
119155
98.35 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
12
0.01 %
SER-FLEXI
553
1.97 %
1928
1.59 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
52
0.04 %
Total(% of all samples)
553
1.97 %
1996
1.65 %
Total
28108
100.00 %
121151
100.00 %
Retests
Total (% of all samples)
xx-xxxx-xxxx
6.03 %
8301
6.85 %
England
1314
4.67 %
6560
5.41 %
Wales
363
1.29 %
xx-xxxx-xxxx
1.39 %
Scotland
19
0.07 %
60
0.05 %
Resamples
Total (% of all samples)
1399
4.98 %
7198
5.94 %
England
1102
3.92 %
5769
4.76 %
Wales
279
0.99 %
1372
1.13 %
Scotland
18
0.06 %
57
0.05 %
Rejects
Total (% of all samples)
116
0.41 %
455
0.38 %
England
88
0.31 %
359
0.30 %
Wales
26
0.09 %
86
0.07 %
Scotland
2
0.01 %
10
0.01 %
Table 1. Monthly Totals for April 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
13475
47.9 %
PAR-CUL-P
1876
6.7 %
PAR-IR-EX
25
0.1 %
PAR-IR-SEV
231
0.8 %
PAR-NEW-IN
6257
22.3 %
PAR-OTHER
499
1.8 %
PAR-PERSIS
2521
9.0 %
PAR-RAPID
87
0.3 %
PAR-RECUR
2584
9.2 %
SER-FLEXI
553
2.0 %
Total
28108
100.00 %
Figure 1. Number of samples submitted in each category during April 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
7000
Ayrshire
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Lancashire
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during April 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
3
362
1.29 %
30
8.29 %
310
85.64 %
23
6.35 %
21
5.80 %
1
0.28 %
PAR-CUL-P
2
493
1.75 %
28
5.68 %
435
88.24 %
37
7.51 %
30
6.09 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
8
1,151
4.09 %
44
3.82 %
1035
89.92 %
83
7.21 %
70
6.08 %
2
0.17 %
PAR-CUL-P
1
221
0.79 %
2
0.90 %
213
96.38 %
8
3.62 %
6
2.71 %
0
0.00 %
PAR-PERSIS
1
21
0.07 %
1
4.76 %
13
61.90 %
8
38.10 %
7
33.33 %
0
0.00 %
Devon
PAR-CUL-N
36
4,682
16.66 %
352
7.52 %
4108
87.74 %
250
5.34 %
215
4.59 %
7
0.15 %
PAR-CUL-P
3
878
3.12 %
53
6.04 %
774
88.15 %
65
7.40 %
50
5.69 %
1
0.11 %
PAR-OTHER
1
35
0.12 %
2
5.71 %
32
91.43 %
2
5.71 %
1
2.86 %
0
0.00 %
PAR-PERSIS
1
406
1.44 %
21
5.17 %
363
89.41 %
24
5.91 %
22
5.42 %
0
0.00 %
Dorset
PAR-CUL-N
9
1,292
4.60 %
76
5.88 %
1147
88.78 %
85
6.58 %
67
5.19 %
2
0.15 %
Gloucs
PAR-CUL-N
17
2,072
7.37 %
104
5.02 %
1800
86.87 %
185
8.93 %
165
7.96 %
3
0.14 %
PAR-CUL-P
1
184
0.65 %
35
19.02 %
144
78.26 %
7
3.80 %
5
2.72 %
0
0.00 %
Heref
PAR-CUL-N
1
12
0.04 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Shropshire
PAR-CUL-N
4
427
1.52 %
30
7.03 %
379
88.76 %
15
3.51 %
11
2.58 %
7
1.64 %
PAR-OTHER
3
15
0.05 %
0
0.00 %
14
93.33 %
1
6.67 %
0
0.00 %
1
6.67 %
Somerset excl N
PAR-CUL-N
9
896
3.19 %
98
10.94 %
758
84.60 %
41
4.58 %
36
4.02 %
4
0.45 %
Staffs
PAR-CUL-N
8
863
3.07 %
21
2.43 %
801
92.82 %
45
5.21 %
41
4.75 %
0
0.00 %
PAR-NEW-IN
2
43
0.15 %
2
4.65 %
35
81.40 %
6
13.95 %
6
13.95 %
0
0.00 %
Wiltshire
PAR-CUL-N
1
155
0.55 %
12
7.74 %
126
81.29 %
17
10.97 %
17
10.97 %
0
0.00 %
PAR-CUL-P
2
78
0.28 %
2
2.56 %
69
88.46 %
5
6.41 %
3
3.85 %
4
5.13 %
PAR-PERSIS
1
4
0.01 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
England - Edge
Berks
PAR-CUL-P
1
22
0.08 %
0
0.00 %
21
95.45 %
1
4.55 %
1
4.55 %
0
0.00 %
PAR-NEW-IN
1
52
0.19 %
5
9.62 %
43
82.69 %
5
9.62 %
4
7.69 %
0
0.00 %
PAR-PERSIS
2
94
0.33 %
8
8.51 %
85
90.43 %
2
2.13 %
1
1.06 %
0
0.00 %
Bucks
PAR-NEW-IN
1
300
1.07 %
9
3.00 %
285
95.00 %
14
4.67 %
5
1.67 %
1
0.33 %
PAR-PERSIS
2
352
1.25 %
5
1.42 %
330
93.75 %
20
5.68 %
17
4.83 %
0
0.00 %
Table 2a. Summary by County for April 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Cheshire
PAR-CUL-N
12
1,515
5.39 %
31
2.05 %
1431
94.46 %
65
4.29 %
53
3.50 %
0
0.00 %
PAR-NEW-IN
5
1,336
4.75 %
30
2.25 %
1214
90.87 %
107
8.01 %
91
6.81 %
1
0.07 %
Derbyshire
PAR-CUL-N
3
48
0.17 %
4
8.33 %
41
85.42 %
3
6.25 %
3
6.25 %
0
0.00 %
PAR-NEW-IN
14
862
3.07 %
25
2.90 %
753
87.35 %
41
4.76 %
34
3.94 %
50
5.80 %
E Sussex
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Hampshire
PAR-NEW-IN
2
133
0.47 %
4
3.01 %
124
93.23 %
7
5.26 %
5
3.76 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
4
194
0.69 %
12
6.19 %
172
88.66 %
10
5.15 %
10
5.15 %
0
0.00 %
Northants
PAR-NEW-IN
5
415
1.48 %
5
1.20 %
389
93.73 %
35
8.43 %
21
5.06 %
0
0.00 %
PAR-PERSIS
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Oxon
PAR-NEW-IN
2
3
0.01 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-PERSIS
3
627
2.23 %
20
3.19 %
583
92.98 %
29
4.63 %
24
3.83 %
0
0.00 %
Warks
PAR-NEW-IN
4
162
0.58 %
5
3.09 %
150
92.59 %
11
6.79 %
7
4.32 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
35
0.12 %
1
2.86 %
23
65.71 %
11
31.43 %
11
31.43 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
1
160
0.57 %
4
2.50 %
151
94.38 %
8
5.00 %
5
3.13 %
0
0.00 %
Lancashire
PAR-NEW-IN
5
563
2.00 %
17
3.02 %
511
90.76 %
32
5.68 %
31
5.51 %
4
0.71 %
Lincs
PAR-NEW-IN
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Suffolk
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
186
21172
75.32 %
1098
5.19 %
18884
89.19 %
1314
6.21 %
1102
5.20 %
88
0.42 %
Wales - High TB
Carms
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
15
0.05 %
5
33.33 %
9
60.00 %
1
6.67 %
1
6.67 %
0
0.00 %
PAR-OTHER
2
38
0.14 %
14
36.84 %
23
60.53 %
3
7.89 %
1
2.63 %
0
0.00 %
PAR-PERSIS
2
81
0.29 %
0
0.00 %
76
93.83 %
5
6.17 %
5
6.17 %
0
0.00 %
PAR-RAPID
8
19
0.07 %
11
57.89 %
7
36.84 %
1
5.26 %
1
5.26 %
0
0.00 %
PAR-RECUR
3
34
0.12 %
2
5.88 %
30
88.24 %
3
8.82 %
2
5.88 %
0
0.00 %
Ceredigion
PAR-IR-SEV
6
31
0.11 %
11
35.48 %
18
58.06 %
2
6.45 %
1
3.23 %
1
3.23 %
PAR-PERSIS
3
170
0.60 %
11
6.47 %
141
82.94 %
24
14.12 %
18
10.59 %
0
0.00 %
PAR-RAPID
6
18
0.06 %
6
33.33 %
12
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
147
0.52 %
13
8.84 %
129
87.76 %
5
3.40 %
4
2.72 %
1
0.68 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Gwent
PAR-IR-SEV
2
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
257
0.91 %
17
6.61 %
231
89.88 %
10
3.89 %
9
3.50 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
2
15
0.05 %
1
6.67 %
14
93.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
16
88
0.31 %
13
14.77 %
71
80.68 %
5
5.68 %
4
4.55 %
0
0.00 %
PAR-OTHER
4
19
0.07 %
0
0.00 %
16
84.21 %
3
15.79 %
3
15.79 %
0
0.00 %
PAR-PERSIS
14
458
1.63 %
29
6.33 %
400
87.34 %
37
8.08 %
26
5.68 %
3
0.66 %
PAR-RAPID
12
25
0.09 %
11
44.00 %
13
52.00 %
3
12.00 %
1
4.00 %
0
0.00 %
PAR-RECUR
7
1,879
6.68 %
74
3.94 %
1763
93.83 %
47
2.50 %
33
1.76 %
9
0.48 %
SER-FLEXI
9
222
0.79 %
16
7.21 %
195
87.84 %
13
5.86 %
8
3.60 %
3
1.35 %
Powys
PAR-IR-EX
2
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
18
0.06 %
12
66.67 %
6
33.33 %
2
11.11 %
0
0.00 %
0
0.00 %
PAR-OTHER
2
68
0.24 %
1
1.47 %
64
94.12 %
6
8.82 %
1
1.47 %
2
2.94 %
PAR-PERSIS
1
4
0.01 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RAPID
3
6
0.02 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
452
1.61 %
20
4.42 %
409
90.49 %
22
4.87 %
21
4.65 %
2
0.44 %
SER-FLEXI
3
34
0.12 %
2
5.88 %
29
85.29 %
4
11.76 %
3
8.82 %
0
0.00 %
S Wales
PAR-IR-SEV
1
18
0.06 %
4
22.22 %
14
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
1
218
0.78 %
11
5.05 %
198
90.83 %
13
5.96 %
8
3.67 %
1
0.46 %
PAR-RAPID
2
4
0.01 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.78 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
2
41
0.15 %
13
31.71 %
26
63.41 %
3
7.32 %
2
4.88 %
0
0.00 %
Ceredigion
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
5
55
0.20 %
12
21.82 %
40
72.73 %
5
9.09 %
3
5.45 %
0
0.00 %
PAR-NEW-IN
11
716
2.55 %
16
2.23 %
669
93.44 %
37
5.17 %
29
4.05 %
2
0.28 %
PAR-OTHER
2
102
0.36 %
4
3.92 %
93
91.18 %
8
7.84 %
5
4.90 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-RAPID
3
5
0.02 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
108
0.38 %
16
14.81 %
84
77.78 %
12
11.11 %
8
7.41 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.01 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
714
2.54 %
26
3.64 %
643
90.06 %
48
6.72 %
45
6.30 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
5
0.02 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
7
249
0.89 %
11
4.42 %
215
86.35 %
24
9.64 %
23
9.24 %
0
0.00 %
PAR-PERSIS
1
42
0.15 %
2
4.76 %
39
92.86 %
0
0.00 %
0
0.00 %
1
2.38 %
Wales
179
6622
23.56 %
412
6.22 %
5905
89.17 %
363
5.48 %
279
4.21 %
26
0.39 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
2
314
1.12 %
61
19.43 %
233
74.20 %
19
6.05 %
18
5.73 %
2
0.64 %
Scotland
2
314
1.12 %
61
19.43 %
233
74.20 %
19
6.05 %
18
5.73 %
2
0.64 %
Grand Total
Sum:
367
28108
1571
25022
xx-xxxx-xxxx
1399
116
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
5
867
0.72 %
66
7.61 %
766
88.35 %
39
4.50 %
32
3.69 %
3
0.35 %
PAR-CUL-P
2
493
0.41 %
28
5.68 %
435
88.24 %
37
7.51 %
30
6.09 %
0
0.00 %
PAR-PERSIS
7
721
0.60 %
131
18.17 %
510
70.74 %
80
11.10 %
71
9.85 %
9
1.25 %
SER-ANOM
1
12
0.01 %
0
0.00 %
9
75.00 %
2
16.67 %
2
16.67 %
1
8.33 %
Corn & Scilly
PAR-CUL-N
50
5,116
4.22 %
237
4.63 %
4376
85.54 %
547
10.69 %
490
9.58 %
13
0.25 %
PAR-CUL-P
6
1,089
0.90 %
36
3.31 %
980
89.99 %
78
7.16 %
71
6.52 %
2
0.18 %
PAR-PERSIS
3
590
0.49 %
30
5.08 %
532
90.17 %
31
5.25 %
28
4.75 %
0
0.00 %
Devon
PAR-CUL-N
169
18,263
15.07 %
1322
7.24 %
15923
87.19 %
1077
5.90 %
953
5.22 %
65
0.36 %
PAR-CUL-P
7
1,034
0.85 %
60
5.80 %
914
88.39 %
76
7.35 %
59
5.71 %
1
0.10 %
PAR-NEW-IN
7
596
0.49 %
26
4.36 %
536
89.93 %
33
5.54 %
31
5.20 %
3
0.50 %
PAR-OTHER
1
35
0.03 %
2
5.71 %
32
91.43 %
2
5.71 %
1
2.86 %
0
0.00 %
PAR-PERSIS
2
423
0.35 %
22
5.20 %
378
89.36 %
25
5.91 %
23
5.44 %
0
0.00 %
Dorset
PAR-CUL-N
38
3,640
3.00 %
242
6.65 %
3204
88.02 %
227
6.24 %
189
5.19 %
5
0.14 %
Gloucs
PAR-CUL-N
45
4,734
3.91 %
264
5.58 %
4095
86.50 %
389
8.22 %
344
7.27 %
31
0.65 %
PAR-CUL-P
1
184
0.15 %
35
19.02 %
144
78.26 %
7
3.80 %
5
2.72 %
0
0.00 %
Heref
PAR-CUL-N
9
596
0.49 %
44
7.38 %
487
81.71 %
48
8.05 %
46
7.72 %
19
3.19 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
110
0.09 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
SER-OTHER
1
52
0.04 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
23
4,136
3.41 %
170
4.11 %
3743
90.50 %
238
5.75 %
212
5.13 %
11
0.27 %
PAR-OTHER
5
523
0.43 %
20
3.82 %
488
93.31 %
17
3.25 %
7
1.34 %
8
1.53 %
Somerset excl N
PAR-CUL-N
40
3,702
3.06 %
339
9.16 %
3220
86.98 %
176
4.75 %
133
3.59 %
10
0.27 %
PAR-NEW-IN
2
370
0.31 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.39 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
Staffs
PAR-CUL-N
40
6,466
5.34 %
239
3.70 %
5793
89.59 %
476
7.36 %
432
6.68 %
2
0.03 %
PAR-CUL-P
3
26
0.02 %
2
7.69 %
18
69.23 %
6
23.08 %
6
23.08 %
0
0.00 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
2
43
0.04 %
2
4.65 %
35
81.40 %
6
13.95 %
6
13.95 %
0
0.00 %
Wiltshire
PAR-CUL-N
21
2,136
1.76 %
182
8.52 %
1807
84.60 %
164
7.68 %
141
6.60 %
6
0.28 %
PAR-CUL-P
4
429
0.35 %
17
3.96 %
383
89.28 %
20
4.66 %
12
2.80 %
17
3.96 %
PAR-NEW-IN
2
191
0.16 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
3
211
0.17 %
17
8.06 %
182
86.26 %
13
6.16 %
12
5.69 %
0
0.00 %
Worcs
PAR-CUL-N
2
128
0.11 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
England - Edge
Berks
PAR-CUL-P
2
319
0.26 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
8
408
0.34 %
27
6.62 %
348
85.29 %
38
9.31 %
33
8.09 %
0
0.00 %
PAR-PERSIS
2
94
0.08 %
8
8.51 %
85
90.43 %
2
2.13 %
1
1.06 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
8
1,359
1.12 %
30
2.21 %
1282
94.33 %
60
4.42 %
46
3.38 %
1
0.07 %
PAR-PERSIS
2
352
0.29 %
5
1.42 %
330
93.75 %
20
5.68 %
17
4.83 %
0
0.00 %
Cheshire
PAR-CUL-N
31
3,824
3.16 %
113
2.96 %
3555
92.97 %
182
4.76 %
155
4.05 %
1
0.03 %
PAR-NEW-IN
19
3,002
2.48 %
96
3.20 %
2743
91.37 %
199
6.63 %
162
5.40 %
1
0.03 %
PAR-PERSIS
2
14
0.01 %
1
7.14 %
9
64.29 %
3
21.43 %
3
21.43 %
1
7.14 %
Derbyshire
PAR-CUL-N
7
985
0.81 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
63
6,889
5.69 %
297
4.31 %
5872
85.24 %
731
10.61 %
653
9.48 %
67
0.97 %
E Sussex
PAR-CUL-N
4
709
0.59 %
74
10.44 %
586
82.65 %
38
5.36 %
34
4.80 %
15
2.12 %
PAR-NEW-IN
4
56
0.05 %
8
14.29 %
36
64.29 %
14
25.00 %
11
19.64 %
1
1.79 %
Hampshire
PAR-CUL-N
2
217
0.18 %
14
6.45 %
199
91.71 %
5
2.30 %
4
1.84 %
0
0.00 %
PAR-NEW-IN
12
1,892
1.56 %
62
3.28 %
xx-xxxx-xxxx
89.43 %
150
7.93 %
136
7.19 %
2
0.11 %
PAR-PERSIS
1
76
0.06 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
22
2,472
2.04 %
118
4.77 %
2211
89.44 %
164
6.63 %
143
5.78 %
0
0.00 %
Northants
PAR-NEW-IN
22
1,596
1.32 %
49
3.07 %
1453
91.04 %
115
7.21 %
94
5.89 %
0
0.00 %
PAR-PERSIS
2
171
0.14 %
2
1.17 %
159
92.98 %
11
6.43 %
10
5.85 %
0
0.00 %
Notts
PAR-NEW-IN
1
12
0.01 %
0
0.00 %
9
75.00 %
3
25.00 %
3
25.00 %
0
0.00 %
Oxon
PAR-NEW-IN
32
2,074
1.71 %
75
3.62 %
1862
89.78 %
148
7.14 %
133
6.41 %
4
0.19 %
PAR-PERSIS
5
791
0.65 %
28
3.54 %
733
92.67 %
35
4.42 %
30
3.79 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Warks
PAR-NEW-IN
36
2,901
2.39 %
116
4.00 %
2609
89.93 %
187
6.45 %
166
5.72 %
10
0.34 %
PAR-PERSIS
2
29
0.02 %
3
10.34 %
18
62.07 %
8
27.59 %
8
27.59 %
0
0.00 %
England - LRA
Cleveland
PAR-NEW-IN
2
194
0.16 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
13
2,162
1.78 %
68
3.15 %
1980
91.58 %
125
5.78 %
104
4.81 %
10
0.46 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.08 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
6
540
0.45 %
18
3.33 %
503
93.15 %
24
4.44 %
19
3.52 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.09 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Lancashire
PAR-NEW-IN
7
605
0.50 %
18
2.98 %
549
90.74 %
35
5.79 %
34
5.62 %
4
0.66 %
Lincs
PAR-NEW-IN
12
1,134
0.94 %
41
3.62 %
1015
89.51 %
81
7.14 %
72
6.35 %
6
0.53 %
N Yorks
PAR-NEW-IN
10
420
0.35 %
27
6.43 %
353
84.05 %
45
10.71 %
39
9.29 %
1
0.24 %
Suffolk
PAR-NEW-IN
4
328
0.27 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.17 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
859
93451
77.14 %
5037
5.39 %
82286
88.05 %
6560
7.02 %
5769
6.17 %
359
0.38 %
Wales - High TB
Carms
PAR-IR-EX
5
11
0.01 %
1
9.09 %
7
63.64 %
2
18.18 %
2
18.18 %
1
9.09 %
PAR-IR-SEV
9
55
0.05 %
18
32.73 %
33
60.00 %
5
9.09 %
4
7.27 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
12
312
0.26 %
38
12.18 %
254
81.41 %
22
7.05 %
18
5.77 %
2
0.64 %
PAR-PERSIS
16
878
0.72 %
54
6.15 %
788
89.75 %
47
5.35 %
36
4.10 %
0
0.00 %
PAR-RAPID
32
61
0.05 %
21
34.43 %
39
63.93 %
1
1.64 %
1
1.64 %
0
0.00 %
PAR-RECUR
5
245
0.20 %
9
3.67 %
225
91.84 %
13
5.31 %
10
4.08 %
1
0.41 %
SER-FLEXI
3
59
0.05 %
10
xx-xxxx-xxxx %
48
81.36 %
3
5.08 %
1
1.69 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
5
0.00 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
12
68
0.06 %
22
32.35 %
43
63.24 %
3
4.41 %
2
2.94 %
1
1.47 %
PAR-OTHER
2
39
0.03 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
11
930
0.77 %
60
6.45 %
815
87.63 %
59
6.34 %
42
4.52 %
13
1.40 %
PAR-RAPID
17
52
0.04 %
13
25.00 %
38
73.08 %
2
3.85 %
1
1.92 %
0
0.00 %
PAR-RECUR
1
5
0.00 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
4
147
0.12 %
13
8.84 %
129
87.76 %
5
3.40 %
4
2.72 %
1
0.68 %
Gwent
PAR-IR-SEV
3
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
159
0.13 %
9
5.66 %
146
91.82 %
7
4.40 %
4
2.52 %
0
0.00 %
PAR-PERSIS
8
355
0.29 %
24
6.76 %
316
89.01 %
15
4.23 %
14
3.94 %
1
0.28 %
PAR-RAPID
4
12
0.01 %
3
25.00 %
9
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-IR-EX
7
44
0.04 %
7
15.91 %
37
84.09 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
34
287
0.24 %
60
20.91 %
216
75.26 %
13
4.53 %
11
3.83 %
0
0.00 %
PAR-OTHER
29
1,288
1.06 %
77
5.98 %
1153
89.52 %
66
5.12 %
55
4.27 %
3
0.23 %
PAR-PERSIS
65
4,183
3.45 %
156
3.73 %
3876
92.66 %
194
4.64 %
145
3.47 %
6
0.14 %
PAR-RAPID
64
216
0.18 %
61
28.24 %
150
69.44 %
8
3.70 %
5
2.31 %
0
0.00 %
PAR-RECUR
16
2,878
2.38 %
117
4.07 %
2663
92.53 %
107
3.72 %
87
3.02 %
11
0.38 %
SER-FLEXI
27
701
0.58 %
52
7.42 %
630
89.87 %
23
3.28 %
15
2.14 %
4
0.57 %
Powys
PAR-IR-EX
6
49
0.04 %
17
34.69 %
28
57.14 %
5
10.20 %
4
8.16 %
0
0.00 %
PAR-IR-SEV
10
59
0.05 %
17
28.81 %
41
69.49 %
3
5.08 %
1
1.69 %
0
0.00 %
PAR-NEW-IN
1
239
0.20 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
13
799
0.66 %
41
5.13 %
709
88.74 %
58
7.26 %
43
5.38 %
6
0.75 %
PAR-PERSIS
23
912
0.75 %
29
3.18 %
801
87.83 %
79
8.66 %
74
8.11 %
8
0.88 %
PAR-RAPID
11
27
0.02 %
2
7.41 %
25
92.59 %
1
3.70 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
452
0.37 %
20
4.42 %
409
90.49 %
22
4.87 %
21
4.65 %
2
0.44 %
SER-FLEXI
6
115
0.09 %
17
14.78 %
89
77.39 %
12
10.43 %
9
7.83 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.85 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
1
18
0.01 %
4
22.22 %
14
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
67
0.06 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.11 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.01 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
1
218
0.18 %
11
5.05 %
198
90.83 %
13
5.96 %
8
3.67 %
1
0.46 %
PAR-PERSIS
5
422
0.35 %
32
7.58 %
369
87.44 %
26
6.16 %
21
4.98 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
3
5
0.00 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.18 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
3
84
0.07 %
22
26.19 %
59
70.24 %
6
7.14 %
3
3.57 %
0
0.00 %
Ceredigion
PAR-OTHER
3
106
0.09 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.06 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
SER-FLEXI
3
148
0.12 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.39 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
8
73
0.06 %
14
19.18 %
54
73.97 %
7
9.59 %
5
6.85 %
0
0.00 %
PAR-NEW-IN
40
2,858
2.36 %
125
4.37 %
2539
88.84 %
217
7.59 %
190
6.65 %
4
0.14 %
PAR-OTHER
4
105
0.09 %
4
3.81 %
94
89.52 %
10
9.52 %
7
6.67 %
0
0.00 %
PAR-PERSIS
8
448
0.37 %
18
4.02 %
402
89.73 %
38
8.48 %
28
6.25 %
0
0.00 %
PAR-RAPID
7
36
0.03 %
8
22.22 %
27
75.00 %
1
2.78 %
1
2.78 %
0
0.00 %
PAR-RECUR
1
104
0.09 %
19
18.27 %
83
79.81 %
2
1.92 %
2
1.92 %
0
0.00 %
SER-FLEXI
6
149
0.12 %
19
12.75 %
122
81.88 %
13
8.72 %
8
5.37 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
11
1,131
0.93 %
38
3.36 %
1000
88.42 %
102
9.02 %
92
8.13 %
1
0.09 %
PAR-PERSIS
1
14
0.01 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
92
0.08 %
6
6.52 %
81
88.04 %
7
7.61 %
4
4.35 %
1
1.09 %
S Glamorgan
PAR-RAPID
2
7
0.01 %
5
71.43 %
2
28.57 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
126
0.10 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
300
0.25 %
12
4.00 %
257
85.67 %
40
13.33 %
31
10.33 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
19
0.02 %
5
26.32 %
13
68.42 %
1
5.26 %
1
5.26 %
0
0.00 %
NE Wales
PAR-NEW-IN
24
2,119
1.75 %
110
5.19 %
1845
87.07 %
194
9.16 %
161
7.60 %
3
0.14 %
PAR-OTHER
4
152
0.13 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
1
42
0.03 %
2
4.76 %
39
92.86 %
0
0.00 %
0
0.00 %
1
2.38 %
SER-FLEXI
3
506
0.42 %
46
9.09 %
426
84.19 %
39
7.71 %
34
6.72 %
0
0.00 %
Wales
646
26961
22.25 %
1595
5.92 %
23908
88.68 %
xx-xxxx-xxxx
6.23 %
1372
5.09 %
86
0.32 %
Scotland - Scotland
Argyll & Bute
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
5
715
0.59 %
95
13.29 %
556
77.76 %
58
8.11 %
55
7.69 %
9
1.26 %
Dumfries & G
PAR-NEW-IN
1
8
0.01 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.01 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
8
739
0.61 %
96
12.99 %
576
77.94 %
60
8.12 %
57
7.71 %
10
1.35 %
Grand Total
Sum:
xx-xxxx-xxxx
6728
106770
8301
7198
455
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol April 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
186
21172
100.0%
1098
5.2%
18884
89.2%
1314
6.2%
1102
5.2%
88
0.4%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
186
21172
100.0%
1098
5.2%
18884
89.2%
1314
6.2%
1102
5.2%
88
0.4%
Scotland
Parallel
2
314
100.0%
61
19.4%
233
74.2%
19
6.1%
18
5.7%
2
0.6%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
2
314
100.0%
61
19.4%
233
74.2%
19
6.1%
18
5.7%
2
0.6%
Wales
Parallel
155
6069
91.6%
352
5.8%
5441
89.7%
326
5.4%
254
4.2%
22
0.4%
Serial/Extended
24
553
8.4%
60
10.8%
464
83.9%
37
6.7%
25
4.5%
4
0.7%
Total
179
6622
100.0%
412
6.2%
5905
89.2%
363
5.5%
279
4.2%
26
0.4%
GB
Parallel
343
27555
98.0%
1511
5.5%
24558
89.1%
1659
6.0%
1374
5.0%
112
0.4%
Serial/Extended
24
553
2.0%
60
10.8%
464
83.9%
37
6.7%
25
4.5%
4
0.7%
Total
367
28108
100.0%
1571
5.6%
25022
89.0%
xx-xxxx-xxxx
6.0%
1399
5.0%
116
0.4%
186.00
179.00
2.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
857
93387
99.9%
5037
5.4%
82226
88.0%
6557
7.0%
5766
6.2%
358
0.4%
Serial/Extended
2
64
0.1%
0
0.0 %
60
93.8%
3
4.7%
3
4.7%
1
1.6%
Total
859
93451
100.0%
5037
5.4%
82286
88.1%
6560
7.0%
5769
6.2%
359
0.4%
Scotland
Parallel
7
735
99.5%
96
13.1%
573
78.0%
60
8.2%
57
7.8%
9
1.2%
Serial/Extended
1
4
0.5%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
8
739
100.0%
96
13.0%
576
77.9%
60
8.1%
57
7.7%
10
1.4%
Wales
Parallel
589
25033
92.8%
1405
5.6%
22253
88.9%
1576
6.3%
1295
5.2%
80
0.3%
Serial/Extended
57
1928
7.2%
190
9.9%
1655
85.8%
105
5.4%
77
4.0%
6
0.3%
Total
646
26961
100.0%
1595
5.9%
23908
88.7%
xx-xxxx-xxxx
6.2%
1372
5.1%
86
0.3%
GB
Parallel
xx-xxxx-xxxx
98.4%
6538
5.5% 105052
88.2%
8193
6.9%
7118
6.0%
447
0.4%
Serial/Extended
60
1996
1.6%
190
9.5%
1718
86.1%
108
5.4%
80
4.0%
8
0.4%
Total
xx-xxxx-xxxx
100.0%
6728
5.6% 106770
88.1%
8301
6.9%
7198
5.9%
455
0.4%
859.00
646.00
8.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
24
553
2.0%
60
10.8%
464
83.9%
37
6.7 %
25
4.5 %
4
0.7 %
Parallel New Infection
76
6257
22.3%
233
3.7%
5618
89.8%
416
6.6 %
346
5.5 %
60
1.0 %
Parallel - new infection cull area
111
13475
47.9%
802
6.0%
11945
88.6%
815
6.0 %
702
5.2 %
26
0.2 %
Parallel - Other
16
499
1.8%
32
6.4%
444
89.0%
36
7.2 %
19
3.8 %
4
0.8 %
Parallel Persistent Infection
35
2521
9.0%
114
4.5%
2269
90.0%
164
6.5 %
134
5.3 %
4
0.2 %
Parallel - persistent infection cull area
10
1876
6.7%
120
6.4%
1656
88.3%
123
6.6 %
95
5.1 %
5
0.3 %
Parallel - Rapid Testing Two-times IRs
39
87
0.3%
36
41.4%
49
56.3%
5
5.7 %
2
2.3 %
0
PAR-IR-EX
6
25
0.1%
1
4.0%
24
96.0%
0
0
0
PAR-IR-SEV
35
231
0.8%
57
24.7%
164
71.0%
15
6.5 %
9
3.9 %
1
0.4 %
PAR-RECUR
15
2584
9.2%
116
4.5%
2389
92.5%
85
3.3 %
67
2.6 %
12
0.5 %
Total
367
28108
100.0%
1571
5.6%
25022
89.0%
xx-xxxx-xxxx
6.0 %
1399
5.0 %
116
0.4 %
Table 4a. Summaryof IFN Gamma results by submission reason (April 2021)
England - HRA
Parallel New Infection
2
43
0.2%
2
4.7%
35
81.4%
6
14.0 %
6
14.0 %
0
Parallel - new infection cull area
96
11912
42.4%
767
6.4%
10473
87.9%
747
6.3 %
646
5.4 %
26
0.2 %
Parallel - Other
4
50
0.2%
2
4.0%
46
92.0%
3
6.0 %
1
2.0 %
1
2.0 %
Parallel Persistent Infection
3
431
1.5%
22
5.1%
378
87.7%
34
7.9 %
31
7.2 %
0
Parallel - persistent infection cull area
9
1854
6.6%
120
6.5%
1635
88.2%
122
6.6 %
94
5.1 %
5
0.3 %
England - HRA
114
14290
50.8%
913
6.4%
12567
87.9%
912
6.4 %
778
5.4 %
32
0.2 %
England - Edge
Parallel New Infection
39
3458
12.3%
95
2.7%
3133
90.6%
231
6.7 %
178
5.1 %
52
1.5 %
Parallel - new infection cull area
15
1563
5.6%
35
2.2%
1472
94.2%
68
4.4 %
56
3.6 %
0
Parallel Persistent Infection
8
1076
3.8%
33
3.1%
1001
93.0%
51
4.7 %
42
3.9 %
0
Parallel - persistent infection cull area
1
22
0.1%
0
0.0 %
21
95.5%
1
4.5 %
1
4.5 %
0
England - Edge
63
6119
21.8%
163
2.7%
5627
92.0%
351
5.7 %
277
4.5 %
52
0.8 %
England - LRA
Parallel New Infection
9
763
2.7%
22
2.9%
690
90.4%
51
6.7 %
47
6.2 %
4
0.5 %
England - LRA
9
763
2.7%
22
2.9%
690
90.4%
51
6.7 %
47
6.2 %
4
0.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
16
403
1.4%
31
7.7%
353
87.6%
22
5.5 %
15
3.7 %
4
1.0 %
Parallel - Other
9
129
0.5%
15
11.6%
107
82.9%
12
9.3 %
5
3.9 %
2
1.6 %
Parallel Persistent Infection
21
970
3.5%
57
5.9%
850
87.6%
78
8.0 %
60
6.2 %
3
0.3 %
Parallel - Rapid Testing Two-times IRs
32
72
0.3%
30
41.7%
40
55.6%
5
6.9 %
2
2.8 %
0
PAR-IR-EX
5
21
0.1%
1
4.8%
20
95.2%
0
0
0
PAR-IR-SEV
30
176
0.6%
45
25.6%
124
70.5%
10
5.7 %
6
3.4 %
1
0.6 %
PAR-RECUR
12
2365
8.4%
96
4.1%
2202
93.1%
72
3.0 %
56
2.4 %
11
0.5 %
Wales - High TB
125
4136
14.7%
275
6.6%
3696
89.4%
199
4.8 %
144
3.5 %
21
0.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
149
0.5%
29
19.5%
110
73.8%
15
10.1 %
10
6.7 %
0
Parallel New Infection
17
1430
5.1%
42
2.9%
1312
91.7%
85
5.9 %
74
5.2 %
2
0.1 %
Parallel - Other
3
320
1.1%
15
4.7%
291
90.9%
21
6.6 %
13
4.1 %
1
0.3 %
Parallel Persistent Infection
2
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel - Rapid Testing Two-times IRs
6
14
0.0%
6
42.9%
8
57.1%
0
0
0
PAR-IR-EX
1
4
0.0%
0
0.0 %
4
100.0%
0
0
0
PAR-IR-SEV
5
55
0.2%
12
21.8%
40
72.7%
5
9.1 %
3
5.5 %
0
PAR-RECUR
3
219
0.8%
20
9.1%
187
85.4%
13
5.9 %
11
5.0 %
1
0.5 %
Wales - Intermediate TB
44
2193
7.8%
124
5.7%
1953
89.1%
140
6.4 %
112
5.1 %
4
0.2 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
7
249
0.9%
11
4.4%
215
86.3%
24
9.6 %
23
9.2 %
0
Parallel Persistent Infection
1
42
0.1%
2
4.8%
39
92.9%
0
0
1
2.4 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
10
293
1.0%
13
4.4%
256
87.4%
24
8.2 %
23
7.8 %
1
0.3 %
Scotland - Scotland
Parallel New Infection
2
314
1.1%
61
19.4%
233
74.2%
19
6.1 %
18
5.7 %
2
0.6 %
Scotland - Scotland
2
314
1.1%
61
19.4%
233
74.2%
19
6.1 %
18
5.7 %
2
0.6 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Extended Flexible IFN-GAMMA test
57
1928
1.6%
190
9.9%
1655
85.8%
105
5.4 %
77
4.0 %
6
0.3 %
Parallel - Herd or Group Slaughter
8
1040
0.9%
45
4.3%
952
91.5%
44
4.2 %
38
3.7 %
5
0.5 %
Parallel New Infection
402
37632
31.1%
1591
4.2%
33292
88.5%
2979
7.9 %
2609
6.9 %
140
0.4 %
Parallel - new infection cull area
488
55554
45.9%
3373
6.1%
48703
87.7%
3739
6.7 %
3293
5.9 %
185
0.3 %
Parallel - Other
83
4271
3.5%
237
5.5%
3789
88.7%
261
6.1 %
223
5.2 %
22
0.5 %
Parallel Persistent Infection
175
11975
9.9%
641
5.4%
10702
89.4%
720
6.0 %
593
5.0 %
39
0.3 %
Parallel - persistent infection cull area
25
3574
3.0%
181
5.1%
3166
88.6%
234
6.5 %
189
5.3 %
38
1.1 %
Parallel - Rapid Testing Two-times IRs
144
421
0.3%
115
27.3%
298
70.8%
13
3.1 %
8
1.9 %
0
PAR-IR-EX
21
117
0.1%
28
23.9%
82
70.1%
7
6.0 %
6
5.1 %
1
0.9 %
PAR-IR-SEV
77
576
0.5%
135
23.4%
417
72.4%
31
5.4 %
23
4.0 %
1
0.2 %
PAR-RECUR
30
3995
3.3%
192
4.8%
3651
91.4%
165
4.1 %
136
3.4 %
16
0.4 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
xx-xxxx-xxxx
100.0%
6728
5.6%
106770
88.1%
8301
6.9 %
7198
5.9 %
455
0.4 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
1
12
0.0%
0
0.0 %
9
75.0%
2
16.7 %
2
16.7 %
1
8.3 %
Parallel New Infection
13
1200
1.0%
94
7.8%
1040
86.7%
67
5.6 %
63
5.3 %
3
0.3 %
Parallel - new infection cull area
442
49784
41.1%
3117
6.3%
43526
87.4%
3384
6.8 %
2974
6.0 %
167
0.3 %
Parallel - Other
9
1026
0.8%
44
4.3%
910
88.7%
54
5.3 %
64
6.2 %
8
0.8 %
Parallel Persistent Infection
17
2055
1.7%
207
10.1%
xx-xxxx-xxxx
82.2%
165
8.0 %
150
7.3 %
9
0.4 %
Parallel - persistent infection cull area
23
3255
2.7%
178
5.5%
2874
88.3%
224
6.9 %
183
5.6 %
20
0.6 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
227
22661
18.7%
878
3.9%
20117
88.8%
1809
8.0 %
1580
7.0 %
86
0.4 %
Parallel - new infection cull area
45
5752
4.7%
256
4.5%
5171
89.9%
345
6.0 %
309
5.4 %
16
0.3 %
Parallel Persistent Infection
16
1527
1.3%
50
3.3%
1403
91.9%
85
5.6 %
73
4.8 %
1
0.1 %
Parallel - persistent infection cull area
2
319
0.3%
3
0.9%
292
91.5%
10
3.1 %
6
1.9 %
18
5.6 %
England - LRA
Parallel New Infection
62
5790
4.8%
210
3.6%
5198
89.8%
404
7.0 %
354
6.1 %
28
0.5 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
859
93451
77.1%
5037
5.4%
82286
88.1%
6560
7.0 %
5769
6.2 %
359
0.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
40
1022
0.8%
92
9.0%
896
87.7%
43
4.2 %
29
2.8 %
5
0.5 %
Parallel - Herd or Group Slaughter
5
1030
0.9%
44
4.3%
944
91.7%
43
4.2 %
37
3.6 %
5
0.5 %
Parallel New Infection
2
245
0.2%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
62
2664
2.2%
168
6.3%
2354
88.4%
166
6.2 %
131
4.9 %
11
0.4 %
Parallel Persistent Infection
125
7394
6.1%
325
4.4%
6725
91.0%
399
5.4 %
316
4.3 %
28
0.4 %
Parallel - Rapid Testing Two-times IRs
129
370
0.3%
100
27.0%
263
71.1%
12
3.2 %
7
1.9 %
0
PAR-IR-EX
19
109
0.1%
25
22.9%
77
70.6%
7
6.4 %
6
5.5 %
1
0.9 %
PAR-IR-SEV
69
503
0.4%
121
24.1%
363
72.2%
24
4.8 %
18
3.6 %
1
0.2 %
PAR-RECUR
24
3580
3.0%
147
4.1%
3300
92.2%
143
4.0 %
119
3.3 %
14
0.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
12
381
0.3%
47
12.3%
320
84.0%
22
5.8 %
13
3.4 %
1
0.3 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel New Infection
57
4582
3.8%
185
4.0%
4044
88.3%
385
8.4 %
343
7.5 %
10
0.2 %
Parallel - Other
8
429
0.4%
21
4.9%
385
89.7%
33
7.7 %
21
4.9 %
2
0.5 %
Parallel Persistent Infection
16
957
0.8%
57
6.0%
846
88.4%
71
7.4 %
54
5.6 %
0
Parallel - Rapid Testing Two-times IRs
13
49
0.0%
14
28.6%
34
69.4%
1
2.0 %
1
2.0 %
0
PAR-IR-EX
2
8
0.0%
3
37.5%
5
62.5%
0
0
0
PAR-IR-SEV
8
73
0.1%
14
19.2%
54
74.0%
7
9.6 %
5
6.8 %
0
PAR-RECUR
6
415
0.3%
45
10.8%
351
84.6%
22
5.3 %
17
4.1 %
2
0.5 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
5
525
0.4%
51
9.7%
439
83.6%
40
7.6 %
35
6.7 %
0
Parallel - Herd or Group Slaughter
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
34
2419
2.0%
122
5.0%
2102
86.9%
234
9.7 %
192
7.9 %
3
0.1 %
Parallel - Other
4
152
0.1%
4
2.6%
140
92.1%
8
5.3 %
7
4.6 %
1
0.7 %
Parallel Persistent Infection
1
42
0.0%
2
4.8%
39
92.9%
0
0
1
2.4 %
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales
646
26961
22.3%
1595
5.9%
23908
88.7%
xx-xxxx-xxxx
6.2 %
1372
5.1 %
86
0.3 %
Scotland - Scotland
Parallel New Infection
7
735
0.6%
96
13.1%
573
78.0%
60
8.2 %
57
7.8 %
9
1.2 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
8
739
0.6%
96
13.0%
576
77.9%
60
8.1 %
57
7.7 %
10
1.4 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
0
70
0
1
August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
0
65
0
1
October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
3
1
7
0
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
58
0
0
0
0
111
1
0
14
2
1366
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
0
4
11
0
1380
25
69
3
4
March
419
36344
256
1867
137
0
0
0
0
69
0
11
4
0
1558
88
0
0
0
April
361
27065
206
1541
353
844
0
0
0
0
2
0
0
0
0
81
15
0
0
0
Totals (last
12 months)
3907
310986
2309
15004
357
1210
0
0
0
0
1141
16
16
70
2
11065
129
733
6
6
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2113
240859
1385
11095
1012
0
0
0
0
1030
9
11
27
1
8564
8
70
0
2
Scotland
45
4356
23
182
0
0
0
0
0
4
1
1
9
0
40
13
49
3
0
Wales
1749
65771
901
3727
198
0
0
0
0
107
6
4
34
1
2461
108
614
3
4
Totals
3907
310986
2309
15004
1210
0
0
0
0
1141
16
16
70
2
11065
129
733
6
6
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
S Wales
Staffs
Shropshire
Powys
Oxon
NE Wales
Leics & Rut
Heref
Gwynedd
Gr Manchester
E Sussex
Devon
Cumbria
Cheshire
Carms
Berks
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during April 2021 (% submission per County)
County
%Resampled %Retested
Avon
5.96 %
7.02 %
Ayrshire
5.73 %
6.05 %
Berks
3.57 %
4.76 %
Bucks
3.37 %
5.21 %
Carms
4.63 %
6.27 %
Ceredigion
6.27 %
8.45 %
Cheshire
5.05 %
6.03 %
Corn & Scilly
5.96 %
7.11 %
Cumbria
31.43 %
31.43 %
Derbyshire
4.07 %
4.84 %
Devon
4.80 %
5.68 %
Dorset
5.19 %
6.58 %
E Sussex
Gloucs
7.54 %
8.51 %
Gr Manchester
3.13 %
5.00 %
Gwent
3.35 %
3.72 %
Gwynedd
Hampshire
3.76 %
5.26 %
Heref
25.00 %
25.00 %
Lancashire
5.51 %
5.68 %
Leics & Rut
5.15 %
5.15 %
Lincs
NE Wales
5.40 %
6.81 %
Northants
5.02 %
8.37 %
Oxon
3.97 %
4.76 %
Pembs
2.77 %
3.99 %
Powys
5.52 %
6.51 %
S Glamorgan
Shropshire
2.49 %
3.62 %
Somerset excl
4.02 %
4.58 %
Staffs
5.19 %
5.63 %
Suffolk
S Wales
Warks
4.32 %
6.79 %
Wiltshire
9.28 %
10.13 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
20826
1098
5.3%
21172
1098
5.2%
Wales
6510
412
6.3%
6622
412
6.2%
Scotland
314
61
19.4%
314
61
19.4%
GB
27650
1571
5.7%
28108
1571
5.6%
Table 6a (i): Number of animals and submissions tested in April 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
89490
5037
5.6%
93451
5037
5.4%
Wales
25761
1595
6.2%
26961
1595
5.9%
Scotland
394
96
24.4%
739
96
13.0%
GB
115639
6728
5.8%
121151
6728
5.6%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of April
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
7439
254
3.4%
7482
254
3.4%
Wales
2923
145
5.0%
2984
145
4.9%
Scotland
461
9
2.0%
462
9
1.9%
GB
10823
408
3.8%
10928
408
3.7%
Table 6a (ii): Number of animals and submissions tested in April 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of April
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
69064
2769
4.0%
73317
2769
3.8%
Wales
16751
983
5.9%
17590
984
5.6%
Scotland
2517
75
3.0%
2662
75
2.8%
GB
88332
3827
4.3%
93569
3828
4.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
1
2
0
0
Total
1
2
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples April 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
1
1
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
1
1
0
0
Wiltshire
PRV-SE
1
10
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
12
92
4
4.35 %
Isle of Man
SER-IOM
3
3
0
0
Isle of Man
SER-IOM
3
3
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
1
4
2
50.0%
BOV-OTHER
1
14
2
14.3%
Ceredigion
BOV-IR-C
4
37
3
8.1%
BOV-OTHER
2
144
3
2.1%
North East Wales
BOV-IR-C
5
63
9
14.3%
BOV-P
1
23
0
0
Pembrokeshire
BOV-IR-C
14
110
14
12.7%
BOV-IR-EX
1
11
0
0
BOV-OTHER
1
150
11
7.3%
BOV-P
2
31
2
6.5%
OTHER
1
1
0
0
Powys
BOV-IR-C
2
23
2
8.7%
BOV-IR-EX
1
3
0
0
BOV-P
1
12
1
8.3%
South Wales
BOV-IR-C
2
22
1
4.5%
Wales
39
648
50
7.7%
All GB
39
648
50
7.7 %
Table 8a Number of Cattle Antibody Tests April 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
Devon
1
95
6
6.3%
England
2
185
6
3.2%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
7
62
15
24.2%
BOV-IR-EX
2
6
2
33.3%
BOV-OTHER
2
26
2
7.7%
BOV-P
1
26
2
7.7%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
11
97
12
12.4%
BOV-OTHER
6
240
16
6.7%
North East Wales
2
24
6
25.0%
BOV-IR-C
6
80
9
11.3%
BOV-IR-EX
1
4
1
25.0%
BOV-P
4
542
9
1.7%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
28
290
43
14.8%
BOV-IR-EX
4
30
5
16.7%
BOV-OTHER
11
584
38
6.5%
BOV-P
6
146
13
8.9%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
10
57
10
17.5%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-IR-EX
2
35
7
20.0%
BOV-OTHER
2
107
18
16.8%
BOV-P
2
47
2
4.3%
South Wales
4
14
5
35.7%
BOV-IR-C
3
32
1
3.1%
Wales
144
2,789
260
9.3%
All GB
146
2,974
266
8.9 %