Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - December 2020
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg tests for
bovine tuberculosis
(TC0651 and TC0751)
Number 172
Report Period 1st - 31st December 2020
Issued 11 January 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
Rapid Testing of twice IR's
(PAR-RAPID)
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other
(PAR-OTHER)
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
Test Criteria

Num
samples
December
2020
%
Total 2020
%
Submissions
324
3512
Samples
Total (%)
24146
100.00 %
284447
100.00 %
England
19851
82.21 %
221766
77.96 %
Wales
3825
15.84 %
56402
19.83 %
Scotland
470
1.95 %
6279
2.21 %
Parallel Tests
PAR-CUL-N
10556
43.72 %
106170
37.33 %
PAR-CUL-P
813
3.37 %
9410
3.31 %
PAR-HERD-S
42
0.17 %
1485
0.52 %
PAR-NEW-IN
9323
38.61 %
117908
41.45 %
PAR-OTHER
237
0.98 %
8057
2.83 %
PAR-PERSIS
2885
11.95 %
35075
12.33 %
PAR-RAPID
155
0.64 %
1678
0.59 %
Total(% of all samples)
24011
99.44 %
279783
98.36 %
Serial/Extended Tests
SER-FLEXI
129
0.53 %
4612
1.62 %
SER-FRAUD
6
0.02 %
25
0.01 %
SER-NSR
0
0.00 %
27
0.01 %
Total(% of all samples)
135
0.56 %
4664
1.64 %
Total
24146
100.00 %
284447
100.00 %
Retests
Total (% of all samples)
1532
6.34 %
17328
6.09 %
England
1248
5.17 %
13576
4.77 %
Wales
251
1.04 %
3369
1.18 %
Scotland
33
0.14 %
383
0.13 %
Resamples
Total (% of all samples)
1358
5.62 %
13573
4.77 %
England
1105
4.58 %
10547
3.71 %
Wales
225
0.93 %
2718
0.96 %
Scotland
28
0.12 %
308
0.11 %
Rejects
Total (% of all samples)
86
0.36 %
2490
0.88 %
England
28
0.12 %
2280
0.80 %
Wales
58
0.24 %
145
0.05 %
Scotland
0
0.00 %
65
0.02 %
Table 1. Monthly Totals for December 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
SER-FLEXI
SER-FRAUD
Breakdown of
Reason for
submission
PAR-CUL-N
10556
43.7 %
PAR-CUL-P
813
3.4 %
PAR-HERD-S
42
0.2 %
PAR-NEW-IN
9323
38.6 %
PAR-OTHER
237
1.0 %
PAR-PERSIS
2885
11.9 %
PAR-RAPID
155
0.6 %
SER-FLEXI
129
0.5 %
SER-FRAUD
6
0.0 %
Total
24146
100.00 %
Figure 1. Number of samples submitted in each category during December 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
Wigtown
Dumfries & G
Argyll & Bute
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Leics & Rut
Hampshire
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Berks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during December 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
Wigtown
Perthshire
Lanarkshire
Kirkcudbright
Dumfries & G
Berwickshire
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northumb
Northants
Norfolk
Lincs
Leics & Rut
Kent
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-NSR
SER-FRAUD
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
1
8
0.03 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
6
0.02 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
11
1,161
4.81 %
73
6.29 %
1031
88.80 %
67
5.77 %
57
4.91 %
0
0.00 %
PAR-CUL-P
3
239
0.99 %
5
2.09 %
221
92.47 %
14
5.86 %
11
4.60 %
2
0.84 %
Devon
PAR-CUL-N
32
3,340
13.83 %
176
5.27 %
2934
87.84 %
265
7.93 %
225
6.74 %
5
0.15 %
PAR-CUL-P
2
67
0.28 %
3
4.48 %
55
82.09 %
9
13.43 %
9
13.43 %
0
0.00 %
Dorset
PAR-CUL-N
6
283
1.17 %
31
10.95 %
243
85.87 %
11
3.89 %
9
3.18 %
0
0.00 %
Gloucs
PAR-CUL-N
13
830
3.44 %
45
5.42 %
718
86.51 %
68
8.19 %
59
7.11 %
8
0.96 %
Shropshire
PAR-CUL-N
4
933
3.86 %
19
2.04 %
869
93.14 %
48
5.14 %
45
4.82 %
0
0.00 %
PAR-PERSIS
3
43
0.18 %
2
4.65 %
31
72.09 %
7
16.28 %
7
16.28 %
3
6.98 %
Somerset excl N
PAR-CUL-N
17
2,235
9.26 %
146
6.53 %
1993
89.17 %
93
4.16 %
94
4.21 %
2
0.09 %
PAR-NEW-IN
1
428
1.77 %
8
1.87 %
394
92.06 %
28
6.54 %
25
5.84 %
1
0.23 %
PAR-OTHER
1
14
0.06 %
4
28.57 %
8
57.14 %
2
14.29 %
2
14.29 %
0
0.00 %
Staffs
PAR-CUL-N
7
1,553
6.43 %
47
3.03 %
1402
90.28 %
121
7.79 %
104
6.70 %
0
0.00 %
PAR-CUL-P
2
507
2.10 %
60
11.83 %
419
82.64 %
31
6.11 %
28
5.52 %
0
0.00 %
PAR-PERSIS
1
359
1.49 %
21
5.85 %
318
88.58 %
25
6.96 %
20
5.57 %
0
0.00 %
Wiltshire
PAR-CUL-N
5
221
0.92 %
11
4.98 %
194
87.78 %
17
7.69 %
16
7.24 %
0
0.00 %
PAR-NEW-IN
4
663
2.75 %
29
4.37 %
621
93.67 %
18
2.71 %
13
1.96 %
0
0.00 %
PAR-PERSIS
3
845
3.50 %
44
5.21 %
778
92.07 %
34
4.02 %
23
2.72 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
1
327
1.35 %
22
6.73 %
292
89.30 %
15
4.59 %
13
3.98 %
0
0.00 %
Cheshire
PAR-NEW-IN
6
1,254
5.19 %
27
2.15 %
1193
95.14 %
42
3.35 %
34
2.71 %
0
0.00 %
Derbyshire
PAR-NEW-IN
6
1,386
5.74 %
48
3.46 %
1217
87.81 %
125
9.02 %
116
8.37 %
5
0.36 %
E Sussex
PAR-NEW-IN
2
275
1.14 %
27
9.82 %
225
81.82 %
24
8.73 %
23
8.36 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
11
0.05 %
0
0.00 %
9
81.82 %
2
18.18 %
2
18.18 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
4
199
0.82 %
9
4.52 %
175
87.94 %
16
8.04 %
15
7.54 %
0
0.00 %
Northants
PAR-NEW-IN
3
386
1.60 %
9
2.33 %
345
89.38 %
33
8.55 %
30
7.77 %
2
0.52 %
Table 2a. Summary by County for December 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Notts
PAR-NEW-IN
1
97
0.40 %
5
5.15 %
79
81.44 %
13
13.40 %
13
13.40 %
0
0.00 %
Oxon
PAR-NEW-IN
7
1,274
5.28 %
75
5.89 %
1132
88.85 %
73
5.73 %
67
5.26 %
0
0.00 %
Warks
PAR-NEW-IN
7
538
2.23 %
50
9.29 %
457
84.94 %
31
5.76 %
31
5.76 %
0
0.00 %
England - LRA
Gr Manchester
PAR-NEW-IN
1
76
0.31 %
0
0.00 %
76
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
N Yorks
PAR-NEW-IN
3
293
1.21 %
9
3.07 %
270
92.15 %
16
5.46 %
14
4.78 %
0
0.00 %
England
159
19851
82.21 %
1005
5.06 %
17713
89.23 %
1248
6.29 %
1105
5.57 %
28
0.14 %
Wales - High TB
Carms
PAR-OTHER
6
37
0.15 %
4
10.81 %
22
59.46 %
11
29.73 %
11
29.73 %
0
0.00 %
PAR-PERSIS
2
10
0.04 %
2
20.00 %
8
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
9
22
0.09 %
3
13.64 %
17
77.27 %
2
9.09 %
2
9.09 %
0
0.00 %
SER-FLEXI
2
2
0.01 %
0
0.00 %
1
50.00 %
0
0.00 %
0
0.00 %
1
50.00 %
Ceredigion
PAR-OTHER
1
5
0.02 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
22
0.09 %
2
9.09 %
19
86.36 %
2
9.09 %
1
4.55 %
0
0.00 %
Gwent
PAR-HERD-S
2
29
0.12 %
2
6.90 %
26
89.66 %
1
3.45 %
1
3.45 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
5
0.02 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-OTHER
13
107
0.44 %
12
11.21 %
83
77.57 %
14
13.08 %
11
10.28 %
1
0.93 %
PAR-PERSIS
15
435
1.80 %
21
4.83 %
379
87.13 %
38
8.74 %
33
7.59 %
2
0.46 %
PAR-RAPID
21
70
0.29 %
17
24.29 %
49
70.00 %
3
4.29 %
3
4.29 %
1
1.43 %
SER-FLEXI
7
82
0.34 %
9
10.98 %
70
85.37 %
3
3.66 %
2
2.44 %
1
1.22 %
Powys
PAR-HERD-S
3
13
0.05 %
1
7.69 %
12
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
4
43
0.18 %
1
2.33 %
40
93.02 %
2
4.65 %
2
4.65 %
0
0.00 %
PAR-OTHER
3
51
0.21 %
3
5.88 %
45
88.24 %
2
3.92 %
2
3.92 %
1
1.96 %
PAR-PERSIS
13
809
3.35 %
21
2.60 %
706
87.27 %
52
6.43 %
46
5.69 %
36
4.45 %
PAR-RAPID
7
15
0.06 %
2
13.33 %
13
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
45
0.19 %
3
6.67 %
41
91.11 %
1
2.22 %
1
2.22 %
0
0.00 %
S Wales
PAR-PERSIS
1
5
0.02 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for December 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-OTHER
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
24
0.10 %
7
29.17 %
16
66.67 %
1
4.17 %
1
4.17 %
0
0.00 %
PAR-RAPID
1
4
0.02 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Ceredigion
PAR-RAPID
2
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
6
517
2.14 %
7
1.35 %
463
89.56 %
46
8.90 %
42
8.12 %
5
0.97 %
PAR-OTHER
1
20
0.08 %
2
10.00 %
17
85.00 %
1
5.00 %
1
5.00 %
0
0.00 %
PAR-PERSIS
8
109
0.45 %
9
8.26 %
97
88.99 %
3
2.75 %
3
2.75 %
0
0.00 %
PAR-RAPID
5
9
0.04 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-NEW-IN
8
879
3.64 %
46
5.23 %
797
90.67 %
36
4.10 %
33
3.75 %
3
0.34 %
PAR-PERSIS
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
1
7
0.03 %
0
0.00 %
5
71.43 %
1
14.29 %
1
14.29 %
1
14.29 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
3
427
1.77 %
5
1.17 %
387
90.63 %
32
7.49 %
29
6.79 %
6
1.41 %
Wales
160
3825
15.84 %
193
5.05 %
3349
87.56 %
251
6.56 %
225
5.88 %
58
1.52 %
Scotland - Scotland
Argyll & Bute
PAR-PERSIS
3
227
0.94 %
27
11.89 %
181
79.74 %
23
10.13 %
19
8.37 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
239
0.99 %
3
1.26 %
228
95.40 %
9
3.77 %
8
3.35 %
0
0.00 %
Wigtown
PAR-NEW-IN
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Scotland
5
470
1.95 %
30
6.38 %
412
87.66 %
33
7.02 %
28
5.96 %
0
0.00 %
Grand Total
Sum:
324
24146
1228
21474
1532
1358
86
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-PERSIS
2
122
0.04 %
14
11.48 %
105
86.07 %
8
6.56 %
3
2.46 %
0
0.00 %
SER-FLEXI
2
73
0.03 %
1
1.37 %
64
87.67 %
8
10.96 %
8
10.96 %
0
0.00 %
SER-FRAUD
2
9
0.00 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
124
14,183
4.99 %
479
3.38 %
12863
90.69 %
993
7.00 %
740
5.22 %
101
0.71 %
PAR-CUL-P
10
2,041
0.72 %
51
2.50 %
1920
94.07 %
87
4.26 %
68
3.33 %
2
0.10 %
PAR-PERSIS
18
3,804
1.34 %
156
4.10 %
3516
92.43 %
186
4.89 %
131
3.44 %
1
0.03 %
SER-FLEXI
2
372
0.13 %
9
2.42 %
354
95.16 %
10
2.69 %
9
2.42 %
0
0.00 %
Devon
PAR-CUL-N
338
37,500
13.18 %
1959
5.22 %
33499
89.33 %
2365
6.31 %
1878
5.01 %
164
0.44 %
PAR-CUL-P
19
1,954
0.69 %
137
7.01 %
xx-xxxx-xxxx
86.59 %
144
7.37 %
119
6.09 %
6
0.31 %
PAR-NEW-IN
1
14
0.00 %
1
7.14 %
10
71.43 %
3
21.43 %
3
21.43 %
0
0.00 %
PAR-OTHER
1
15
0.01 %
9
60.00 %
6
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
92
0.03 %
8
8.70 %
80
86.96 %
8
8.70 %
4
4.35 %
0
0.00 %
SER-FLEXI
6
312
0.11 %
7
2.24 %
288
92.31 %
14
4.49 %
11
3.53 %
6
1.92 %
Dorset
PAR-CUL-N
78
9,028
3.17 %
580
6.42 %
7923
87.76 %
480
5.32 %
374
4.14 %
151
1.67 %
PAR-CUL-P
12
3,059
1.08 %
264
8.63 %
2661
86.99 %
183
5.98 %
133
4.35 %
1
0.03 %
PAR-NEW-IN
2
167
0.06 %
5
2.99 %
156
93.41 %
11
6.59 %
6
3.59 %
0
0.00 %
PAR-PERSIS
2
19
0.01 %
0
0.00 %
16
84.21 %
3
15.79 %
3
15.79 %
0
0.00 %
SER-FLEXI
10
808
0.28 %
52
6.44 %
735
90.97 %
34
4.21 %
21
2.60 %
0
0.00 %
Gloucs
PAR-CUL-N
80
6,937
2.44 %
331
4.77 %
6144
88.57 %
569
8.20 %
450
6.49 %
12
0.17 %
PAR-NEW-IN
1
38
0.01 %
4
10.53 %
32
84.21 %
4
10.53 %
2
5.26 %
0
0.00 %
PAR-OTHER
2
63
0.02 %
1
1.59 %
55
87.30 %
8
12.70 %
7
11.11 %
0
0.00 %
PAR-PERSIS
2
242
0.09 %
14
5.79 %
220
90.91 %
10
4.13 %
8
3.31 %
0
0.00 %
Heref
PAR-CUL-N
18
2,820
0.99 %
102
3.62 %
2614
92.70 %
137
4.86 %
99
3.51 %
5
0.18 %
PAR-NEW-IN
10
687
0.24 %
24
3.49 %
616
89.67 %
63
9.17 %
46
6.70 %
1
0.15 %
PAR-OTHER
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
213
0.07 %
9
4.23 %
172
80.75 %
32
15.02 %
32
15.02 %
0
0.00 %
Table 2b. Summary by County for 2020
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Shropshire
PAR-CUL-N
29
6,472
2.28 %
130
2.01 %
6062
93.67 %
373
5.76 %
277
4.28 %
3
0.05 %
PAR-PERSIS
15
2,459
0.86 %
119
4.84 %
2243
91.22 %
109
4.43 %
92
3.74 %
5
0.20 %
SER-FLEXI
3
389
0.14 %
16
4.11 %
352
90.49 %
27
6.94 %
21
5.40 %
0
0.00 %
Somerset excl N
PAR-CUL-N
138
14,479
5.09 %
632
4.36 %
13318
91.98 %
693
4.79 %
498
3.44 %
31
0.21 %
PAR-CUL-P
3
418
0.15 %
13
3.11 %
391
93.54 %
18
4.31 %
14
3.35 %
0
0.00 %
PAR-NEW-IN
1
428
0.15 %
8
1.87 %
394
92.06 %
28
6.54 %
25
5.84 %
1
0.23 %
PAR-OTHER
2
405
0.14 %
70
17.28 %
319
78.77 %
18
4.44 %
16
3.95 %
0
0.00 %
PAR-PERSIS
3
293
0.10 %
25
8.53 %
258
88.05 %
14
4.78 %
10
3.41 %
0
0.00 %
SER-FLEXI
5
202
0.07 %
7
3.47 %
191
94.55 %
4
1.98 %
4
1.98 %
0
0.00 %
Staffs
PAR-CUL-N
32
4,875
1.71 %
143
2.93 %
4313
88.47 %
302
6.19 %
263
5.39 %
156
3.20 %
PAR-CUL-P
4
697
0.25 %
65
9.33 %
591
84.79 %
46
6.60 %
41
5.88 %
0
0.00 %
PAR-NEW-IN
54
7,500
2.64 %
367
4.89 %
6857
91.43 %
352
4.69 %
274
3.65 %
2
0.03 %
PAR-PERSIS
1
359
0.13 %
21
5.85 %
318
88.58 %
25
6.96 %
20
5.57 %
0
0.00 %
Wiltshire
PAR-CUL-N
74
8,544
3.00 %
240
2.81 %
7841
91.77 %
583
6.82 %
443
5.18 %
20
0.23 %
PAR-CUL-P
7
1,241
0.44 %
69
5.56 %
1096
88.32 %
95
7.66 %
65
5.24 %
11
0.89 %
PAR-NEW-IN
6
788
0.28 %
30
3.81 %
743
94.29 %
22
2.79 %
15
1.90 %
0
0.00 %
PAR-PERSIS
5
949
0.33 %
49
5.16 %
871
91.78 %
41
4.32 %
29
3.06 %
0
0.00 %
SER-FLEXI
2
45
0.02 %
4
8.89 %
41
91.11 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FRAUD
1
16
0.01 %
0
0.00 %
16
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
26
3,110
1.09 %
100
3.22 %
2790
89.71 %
284
9.13 %
220
7.07 %
0
0.00 %
PAR-PERSIS
3
319
0.11 %
2
0.63 %
308
96.55 %
11
3.45 %
9
2.82 %
0
0.00 %
Bucks
PAR-NEW-IN
16
2,010
0.71 %
80
3.98 %
1753
87.21 %
212
10.55 %
176
8.76 %
1
0.05 %
Cheshire
PAR-CUL-N
7
874
0.31 %
14
1.60 %
843
96.45 %
28
3.20 %
17
1.95 %
0
0.00 %
PAR-NEW-IN
161
28,116
9.88 %
567
2.02 %
26364
93.77 %
1464
5.21 %
1135
4.04 %
50
0.18 %
Derbyshire
PAR-NEW-IN
141
17,003
5.98 %
605
3.56 %
14618
85.97 %
1237
7.28 %
1023
6.02 %
757
4.45 %
E Sussex
PAR-NEW-IN
30
2,501
0.88 %
94
3.76 %
2289
91.52 %
136
5.44 %
118
4.72 %
0
0.00 %
Hampshire
PAR-CUL-N
2
363
0.13 %
15
4.13 %
327
90.08 %
39
10.74 %
19
5.23 %
2
0.55 %
PAR-NEW-IN
35
4,002
1.41 %
117
2.92 %
3690
92.20 %
280
7.00 %
195
4.87 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
52
4,939
1.74 %
188
3.81 %
4491
90.93 %
323
6.54 %
242
4.90 %
18
0.36 %
Northants
PAR-NEW-IN
29
2,477
0.87 %
81
3.27 %
2252
90.92 %
169
6.82 %
139
5.61 %
5
0.20 %
Notts
PAR-NEW-IN
7
463
0.16 %
9
1.94 %
429
92.66 %
30
6.48 %
24
5.18 %
1
0.22 %
Oxon
PAR-NEW-IN
91
9,085
3.19 %
420
4.62 %
7953
87.54 %
567
6.24 %
424
4.67 %
288
3.17 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Warks
PAR-CUL-N
1
95
0.03 %
0
0.00 %
91
95.79 %
6
6.32 %
4
4.21 %
0
0.00 %
PAR-NEW-IN
75
6,562
2.31 %
215
3.28 %
5934
90.43 %
402
6.13 %
309
4.71 %
104
1.58 %
PAR-PERSIS
3
496
0.17 %
20
4.03 %
460
92.74 %
23
4.64 %
16
3.23 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
384
0.13 %
4
1.04 %
363
94.53 %
32
8.33 %
17
4.43 %
0
0.00 %
Cleveland
PAR-NEW-IN
1
50
0.02 %
2
4.00 %
48
96.00 %
1
2.00 %
0
0.00 %
0
0.00 %
Cumbria
PAR-NEW-IN
4
51
0.02 %
1
1.96 %
42
82.35 %
9
17.65 %
8
15.69 %
0
0.00 %
PAR-PERSIS
1
33
0.01 %
1
3.03 %
28
84.85 %
4
12.12 %
4
12.12 %
0
0.00 %
Essex
PAR-NEW-IN
3
265
0.09 %
3
1.13 %
252
95.09 %
11
4.15 %
10
3.77 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
8
412
0.14 %
6
1.46 %
190
46.12 %
6
1.46 %
6
1.46 %
210
50.97 %
Humberside
PAR-NEW-IN
2
78
0.03 %
7
8.97 %
66
84.62 %
7
8.97 %
5
6.41 %
0
0.00 %
Kent
PAR-NEW-IN
1
58
0.02 %
1
1.72 %
53
91.38 %
4
6.90 %
4
6.90 %
0
0.00 %
Lincs
PAR-NEW-IN
8
360
0.13 %
15
4.17 %
327
90.83 %
19
5.28 %
18
5.00 %
0
0.00 %
PAR-PERSIS
2
34
0.01 %
0
0.00 %
31
91.18 %
5
14.71 %
3
8.82 %
0
0.00 %
Norfolk
PAR-NEW-IN
4
49
0.02 %
1
2.04 %
29
59.18 %
6
12.24 %
6
12.24 %
13
26.53 %
Northumb
PAR-NEW-IN
2
307
0.11 %
17
5.54 %
240
78.18 %
40
13.03 %
50
16.29 %
0
0.00 %
N Yorks
PAR-NEW-IN
13
1,142
0.40 %
25
2.19 %
983
86.08 %
56
4.90 %
43
3.77 %
91
7.97 %
Surrey
PAR-NEW-IN
3
429
0.15 %
17
3.96 %
396
92.31 %
18
4.20 %
16
3.73 %
0
0.00 %
W Yorks
PAR-NEW-IN
8
563
0.20 %
4
0.71 %
473
84.01 %
37
6.57 %
25
4.44 %
61
10.83 %
England
1874
221766
77.96 %
8856
3.99 %
200083
90.22 %
13576
6.12 %
10547
4.76 %
2280
1.03 %
Wales - High TB
Carms
PAR-HERD-S
12
678
0.24 %
51
7.52 %
598
88.20 %
45
6.64 %
29
4.28 %
0
0.00 %
PAR-NEW-IN
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
56
1,163
0.41 %
155
13.33 %
951
81.77 %
66
5.67 %
57
4.90 %
0
0.00 %
PAR-PERSIS
67
3,612
1.27 %
264
7.31 %
3183
88.12 %
202
5.59 %
160
4.43 %
5
0.14 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
87
310
0.11 %
57
18.39 %
233
75.16 %
28
9.03 %
20
6.45 %
0
0.00 %
SER-FLEXI
11
115
0.04 %
24
20.87 %
86
74.78 %
3
2.61 %
2
1.74 %
3
2.61 %
Ceredigion
PAR-HERD-S
2
13
0.00 %
0
0.00 %
7
53.85 %
6
46.15 %
6
46.15 %
0
0.00 %
PAR-OTHER
37
756
0.27 %
69
9.13 %
656
86.77 %
41
5.42 %
31
4.10 %
0
0.00 %
PAR-PERSIS
41
1,827
0.64 %
107
5.86 %
1621
88.72 %
108
5.91 %
97
5.31 %
2
0.11 %
PAR-RAPID
46
147
0.05 %
36
24.49 %
105
71.43 %
9
6.12 %
6
4.08 %
0
0.00 %
SER-FLEXI
4
480
0.17 %
39
8.13 %
416
86.67 %
31
6.46 %
25
5.21 %
0
0.00 %
Gwent
PAR-HERD-S
3
32
0.01 %
3
9.38 %
28
87.50 %
1
3.13 %
1
3.13 %
0
0.00 %
PAR-OTHER
3
9
0.00 %
2
22.22 %
6
66.67 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-PERSIS
28
1,463
0.51 %
95
6.49 %
1324
90.50 %
68
4.65 %
43
2.94 %
1
0.07 %
PAR-RAPID
22
119
0.04 %
22
18.49 %
94
78.99 %
5
4.20 %
3
2.52 %
0
0.00 %
SER-FLEXI
9
284
0.10 %
27
9.51 %
251
88.38 %
10
3.52 %
6
2.11 %
0
0.00 %
Pembs
PAR-HERD-S
7
292
0.10 %
11
3.77 %
271
92.81 %
12
4.11 %
10
3.42 %
0
0.00 %
PAR-NEW-IN
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
133
2,996
1.05 %
305
10.18 %
2570
85.78 %
154
5.14 %
115
3.84 %
6
0.20 %
PAR-PERSIS
191
11,943
4.20 %
549
4.60 %
10850
90.85 %
648
5.43 %
530
4.44 %
14
0.12 %
PAR-RAPID
162
685
0.24 %
140
20.44 %
514
75.04 %
36
5.26 %
29
4.23 %
2
0.29 %
SER-FLEXI
32
646
0.23 %
44
6.81 %
578
89.47 %
32
4.95 %
20
3.10 %
4
0.62 %
Powys
PAR-HERD-S
20
285
0.10 %
10
3.51 %
275
96.49 %
3
1.05 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
7
159
0.06 %
7
4.40 %
148
93.08 %
4
2.52 %
4
2.52 %
0
0.00 %
PAR-OTHER
20
1,045
0.37 %
55
5.26 %
903
86.41 %
94
9.00 %
85
8.13 %
2
0.19 %
PAR-PERSIS
75
3,027
1.06 %
139
4.59 %
2630
86.88 %
245
8.09 %
212
7.00 %
46
1.52 %
PAR-RAPID
68
208
0.07 %
29
13.94 %
174
83.65 %
5
2.40 %
4
1.92 %
1
0.48 %
SER-FLEXI
13
321
0.11 %
25
7.79 %
284
88.47 %
22
6.85 %
12
3.74 %
0
0.00 %
S Wales
PAR-PERSIS
3
279
0.10 %
15
5.38 %
262
93.91 %
8
2.87 %
2
0.72 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
3
50.00 %
3
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
36
0.01 %
0
0.00 %
36
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
1
3
0.00 %
0
0.00 %
0
0.00 %
3
100.00 %
3
100.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
5
18
0.01 %
0
0.00 %
16
88.89 %
4
22.22 %
2
11.11 %
0
0.00 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
3
30
0.01 %
5
16.67 %
24
80.00 %
3
10.00 %
1
3.33 %
0
0.00 %
PAR-NEW-IN
1
155
0.05 %
34
21.94 %
119
76.77 %
2
1.29 %
2
1.29 %
0
0.00 %
PAR-OTHER
13
285
0.10 %
24
8.42 %
249
87.37 %
14
4.91 %
12
4.21 %
0
0.00 %
PAR-PERSIS
15
808
0.28 %
56
6.93 %
721
89.23 %
39
4.83 %
31
3.84 %
0
0.00 %
PAR-RAPID
9
16
0.01 %
8
50.00 %
7
43.75 %
1
6.25 %
1
6.25 %
0
0.00 %
SER-FLEXI
2
29
0.01 %
7
24.14 %
20
68.97 %
2
6.90 %
2
6.90 %
0
0.00 %
Ceredigion
PAR-PERSIS
4
171
0.06 %
12
7.02 %
152
88.89 %
8
4.68 %
7
4.09 %
0
0.00 %
PAR-RAPID
7
31
0.01 %
9
29.03 %
22
70.97 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
48
0.02 %
6
12.50 %
40
83.33 %
2
4.17 %
2
4.17 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-NEW-IN
99
9,255
3.25 %
250
2.70 %
8483
91.66 %
614
6.63 %
514
5.55 %
8
0.09 %
PAR-OTHER
27
405
0.14 %
33
8.15 %
352
86.91 %
24
5.93 %
20
4.94 %
0
0.00 %
PAR-PERSIS
28
1,681
0.59 %
101
6.01 %
1524
90.66 %
67
3.99 %
53
3.15 %
3
0.18 %
PAR-RAPID
37
102
0.04 %
23
22.55 %
76
74.51 %
3
2.94 %
3
2.94 %
0
0.00 %
SER-FLEXI
11
430
0.15 %
21
4.88 %
380
88.37 %
37
8.60 %
27
6.28 %
2
0.47 %
Powys
PAR-NEW-IN
27
2,975
1.05 %
89
2.99 %
2743
92.20 %
158
5.31 %
136
4.57 %
7
0.24 %
PAR-OTHER
4
103
0.04 %
1
0.97 %
89
86.41 %
18
17.48 %
13
12.62 %
0
0.00 %
PAR-PERSIS
5
288
0.10 %
4
1.39 %
268
93.06 %
19
6.60 %
16
5.56 %
0
0.00 %
PAR-RAPID
4
5
0.00 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Glamorgan
PAR-PERSIS
2
128
0.04 %
20
15.63 %
93
72.66 %
16
12.50 %
15
11.72 %
0
0.00 %
PAR-RAPID
2
5
0.00 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
13
0.00 %
5
38.46 %
8
61.54 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-PERSIS
4
106
0.04 %
7
6.60 %
94
88.68 %
4
3.77 %
3
2.83 %
2
1.89 %
PAR-RAPID
1
7
0.00 %
1
14.29 %
6
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
153
0.05 %
1
0.65 %
146
95.42 %
7
4.58 %
6
3.92 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2020 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
35
1,777
0.62 %
33
1.86 %
1665
93.70 %
102
5.74 %
75
4.22 %
4
0.23 %
PAR-OTHER
3
9
0.00 %
0
0.00 %
5
55.56 %
4
44.44 %
4
44.44 %
0
0.00 %
PAR-RAPID
3
13
0.00 %
0
0.00 %
10
76.92 %
3
23.08 %
3
23.08 %
0
0.00 %
NE Wales
PAR-HERD-S
1
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-NEW-IN
38
4,323
1.52 %
79
1.83 %
3965
91.72 %
315
7.29 %
246
5.69 %
33
0.76 %
PAR-OTHER
3
14
0.00 %
1
7.14 %
11
78.57 %
2
14.29 %
2
14.29 %
0
0.00 %
PAR-PERSIS
1
40
0.01 %
0
0.00 %
32
80.00 %
10
25.00 %
8
20.00 %
0
0.00 %
PAR-RAPID
4
21
0.01 %
1
4.76 %
20
95.24 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
1575
56402
19.83 %
3117
5.53 %
50422
89.40 %
3369
5.97 %
2718
4.82 %
145
0.26 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
7
588
0.21 %
32
5.44 %
455
77.38 %
41
6.97 %
36
6.12 %
65
11.05 %
PAR-OTHER
7
782
0.27 %
15
1.92 %
700
89.51 %
94
12.02 %
67
8.57 %
0
0.00 %
PAR-PERSIS
3
227
0.08 %
27
11.89 %
181
79.74 %
23
10.13 %
19
8.37 %
0
0.00 %
SER-NSR
2
27
0.01 %
4
14.81 %
21
77.78 %
2
7.41 %
2
7.41 %
0
0.00 %
Ayrshire
PAR-NEW-IN
3
383
0.13 %
1
0.26 %
359
93.73 %
30
7.83 %
23
6.01 %
0
0.00 %
Berwickshire
PAR-NEW-IN
2
78
0.03 %
8
10.26 %
62
79.49 %
8
10.26 %
8
10.26 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
7
1,088
0.38 %
15
1.38 %
1030
94.67 %
47
4.32 %
43
3.95 %
0
0.00 %
Kirkcudbright
PAR-NEW-IN
15
807
0.28 %
22
2.73 %
762
94.42 %
34
4.21 %
23
2.85 %
0
0.00 %
PAR-PERSIS
1
22
0.01 %
0
0.00 %
22
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
3
782
0.27 %
8
1.02 %
736
94.12 %
47
6.01 %
38
4.86 %
0
0.00 %
Perthshire
PAR-NEW-IN
2
357
0.13 %
0
0.00 %
345
96.64 %
14
3.92 %
12
3.36 %
0
0.00 %
Wigtown
PAR-NEW-IN
11
1,138
0.40 %
29
2.55 %
1072
94.20 %
43
3.78 %
37
3.25 %
0
0.00 %
Scotland
63
6279
2.21 %
161
2.56 %
5745
91.50 %
383
6.10 %
308
4.91 %
65
1.04 %
Grand Total
Sum:
xx-xxxx-xxxx
12134
256250
17328
13573
2490
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol December 2020
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
158
19845
100.0%
1005
5.1%
17707
89.2%
1248
6.3%
1105
5.6%
28
0.1%
Serial/Extended
1
6
0.0%
0
0.0 %
6 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
159
19851
100.0%
1005
5.1%
17713
89.2%
1248
6.3%
1105
5.6%
28
0.1%
Scotland
Parallel
5
470
100.0%
30
6.4%
412
87.7%
33
7.0%
28
6.0%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
5
470
100.0%
30
6.4%
412
87.7%
33
7.0%
28
6.0%
0
0.0 %
Wales
Parallel
149
3696
96.6%
181
4.9%
3237
87.6%
247
6.7%
222
6.0%
56
1.5%
Serial/Extended
11
129
3.4%
12
9.3%
112
86.8%
4
3.1%
3
2.3%
2
1.6%
Total
160
3825
100.0%
193
5.0%
3349
87.6%
251
6.6%
225
5.9%
58
1.5%
GB
Parallel
312
24011
99.4%
1216
5.1%
21356
88.9%
1528
6.4%
1355
5.6%
84
0.3%
Serial/Extended
12
135
0.6%
12
8.9%
118
87.4%
4
3.0%
3
2.2%
2
1.5%
Total
324
24146
100.0%
1228
5.1%
21474
88.9%
1532
6.3%
1358
5.6%
86
0.4%
159.00
160.00
5.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.0%
8760
4.0% 198033
90.2%
13479
6.1%
10473
4.8%
2274
1.0%
Serial/Extended
33
2226
1.0%
96
4.3%
2050
92.1%
97
4.4%
74
3.3%
6
0.3%
Total
xx-xxxx-xxxx
100.0%
8856
4.0% 200083
90.2%
13576
6.1%
10547
4.8%
2280
1.0%
Scotland
Parallel
61
6252
99.6%
157
2.5%
5724
91.6%
381
6.1%
306
4.9%
65
1.0%
Serial/Extended
2
27
0.4%
4
0.1%
21
77.8%
2
7.4%
2
7.4%
0
0.0 %
Total
63
6279
100.0%
161
2.6%
5745
91.5%
383
6.1%
308
4.9%
65
1.0%
Wales
Parallel
1486
53991
95.7%
2918
5.4%
48315
89.5%
3230
6.0%
2622
4.9%
136
0.3%
Serial/Extended
89
2411
4.3%
199
8.3%
2107
87.4%
139
5.8%
96
4.0%
9
0.4%
Total
1575
56402
100.0%
3117
5.5%
50422
89.4%
3369
6.0%
2718
4.8%
145
0.3%
GB
Parallel
3388 279783
98.4% 11835
4.2% 252072
90.1%
17090
6.1%
13401
4.8%
2475
0.9%
Serial/Extended
124
4664
1.6%
299
6.4%
4178
89.6%
238
5.1%
172
3.7%
15
0.3%
Total
xx-xxxx-xxxx
100.0% 12134
4.3% 256250
90.1%
17328
6.1%
13573
4.8%
2490
0.9%
#######
#######
63.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
11
129
0.5%
12
9.3%
112
86.8%
4
3.1 %
3
2.3 %
2
1.6 %
Parallel - Herd or Group Slaughter
5
42
0.2%
3
7.1%
38
90.5%
1
2.4 %
1
2.4 %
0
Parallel New Infection
71
9323
38.6%
380
4.1%
8408
90.2%
563
6.0 %
512
5.5 %
23
0.2 %
Parallel - new infection cull area
95
10556
43.7%
548
5.2%
9384
88.9%
690
6.5 %
609
5.8 %
15
0.1 %
Parallel - Other
27
237
1.0%
27
11.4%
181
76.4%
30
12.7 %
27
11.4 %
2
0.8 %
Parallel Persistent Infection
54
2885
11.9%
159
5.5%
2533
87.8%
183
6.3 %
152
5.3 %
41
1.4 %
Parallel - persistent infection cull area
7
813
3.4%
68
8.4%
695
85.5%
54
6.6 %
48
5.9 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
53
155
0.6%
31
20.0%
117
75.5%
7
4.5 %
6
3.9 %
1
0.6 %
Serial - Anomalous Reaction
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Total
324
24146
100.0%
1228
5.1%
21474
88.9%
1532
6.3 %
1358
5.6 %
86
0.4 %
Table 4a. Summaryof IFN Gamma results by submission reason (December 2020)
England - HRA
Parallel New Infection
5
1091
4.5%
37
3.4%
1015
93.0%
46
4.2 %
38
3.5 %
1
0.1 %
Parallel - new infection cull area
95
10556
43.7%
548
5.2%
9384
88.9%
690
6.5 %
609
5.8 %
15
0.1 %
Parallel - Other
1
14
0.1%
4
28.6%
8
57.1%
2
14.3 %
2
14.3 %
0
Parallel Persistent Infection
8
1255
5.2%
67
5.3%
1135
90.4%
66
5.3 %
50
4.0 %
3
0.2 %
Parallel - persistent infection cull area
7
813
3.4%
68
8.4%
695
85.5%
54
6.6 %
48
5.9 %
2
0.2 %
Serial - Anomalous Reaction
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
England - HRA
117
13735
56.9%
724
5.3%
12243
89.1%
858
6.2 %
747
5.4 %
21
0.2 %
England - Edge
Parallel New Infection
38
5747
23.8%
272
4.7%
5124
89.2%
374
6.5 %
344
6.0 %
7
0.1 %
England - Edge
38
5747
23.8%
272
4.7%
5124
89.2%
374
6.5 %
344
6.0 %
7
0.1 %
England - LRA
Parallel New Infection
4
369
1.5%
9
2.4%
346
93.8%
16
4.3 %
14
3.8 %
0
England - LRA
4
369
1.5%
9
2.4%
346
93.8%
16
4.3 %
14
3.8 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
11
129
0.5%
12
9.3%
112
86.8%
4
3.1 %
3
2.3 %
2
1.6 %
Parallel - Herd or Group Slaughter
5
42
0.2%
3
7.1%
38
90.5%
1
2.4 %
1
2.4 %
0
Parallel New Infection
4
43
0.2%
1
2.3%
40
93.0%
2
4.7 %
2
4.7 %
0
Parallel - Other
24
201
0.8%
20
10.0%
155
77.1%
27
13.4 %
24
11.9 %
2
1.0 %
Parallel Persistent Infection
33
1265
5.2%
49
3.9%
1099
86.9%
90
7.1 %
79
6.2 %
38
3.0 %
Parallel - Rapid Testing Two-times IRs
42
134
0.6%
26
19.4%
101
75.4%
7
5.2 %
6
4.5 %
1
0.7 %
Wales - High TB
119
1814
7.5%
111
6.1%
1545
85.2%
131
7.2 %
115
6.3 %
43
2.4 %
Wales - Intermediate TB
Parallel New Infection
14
1396
5.8%
53
3.8%
1260
90.3%
82
5.9 %
75
5.4 %
8
0.6 %
Parallel - Other
2
22
0.1%
3
13.6%
18
81.8%
1
4.5 %
1
4.5 %
0
Parallel Persistent Infection
10
138
0.6%
16
11.6%
118
85.5%
4
2.9 %
4
2.9 %
0
Parallel - Rapid Testing Two-times IRs
10
20
0.1%
5
25.0%
15
75.0%
0
0
0
Wales - Intermediate TB
36
1576
6.5%
77
4.9%
1411
89.5%
87
5.5 %
80
5.1 %
8
0.5 %
Wales - Low TB
Parallel New Infection
4
434
1.8%
5
1.2%
392
90.3%
33
7.6 %
30
6.9 %
7
1.6 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
5
435
1.8%
5
1.1%
393
90.3%
33
7.6 %
30
6.9 %
7
1.6 %
Scotland - Scotland
Parallel New Infection
2
243
1.0%
3
1.2%
231
95.1%
10
4.1 %
9
3.7 %
0
Parallel Persistent Infection
3
227
0.9%
27
11.9%
181
79.7%
23
10.1 %
19
8.4 %
0
Scotland - Scotland
5
470
1.9%
30
6.4%
412
87.7%
33
7.0 %
28
6.0 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
119
4612
1.6%
295
6.4%
4132
89.6%
236
5.1 %
170
3.7 %
15
0.3 %
Parallel - Herd or Group Slaughter
51
1485
0.5%
81
5.5%
1350
90.9%
78
5.3 %
54
3.6 %
0
Parallel New Infection
1057 117908
41.5%
3625
3.1%
106782
90.6%
7292
6.2 %
5781
4.9 %
1720
1.5 %
Parallel - new infection cull area
921 106170
37.3%
4625
4.4%
95838
90.3%
6568
6.2 %
5062
4.8 %
645
0.6 %
Parallel - Other
313
8057
2.8%
740
9.2%
6876
85.3%
541
6.7 %
433
5.4 %
8
0.1 %
Parallel Persistent Infection
534
35075
12.3%
1834
5.2%
31600
90.1%
1948
5.6 %
1562
4.5 %
79
0.2 %
Parallel - persistent infection cull area
55
9410
3.3%
599
6.4%
8351
88.7%
573
6.1 %
440
4.7 %
20
0.2 %
Parallel - Rapid Testing Two-times IRs
457
1678
0.6%
331
19.7%
1275
76.0%
90
5.4 %
69
4.1 %
3
0.2 %
Serial - Anomalous Reaction
3
25
0.0%
0
0.0 %
25
100.0%
0
0
0
Serial - Suspected NSR Herd
2
27
0.0%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Total
xx-xxxx-xxxx
100.0%
12134
4.3%
256250
90.1%
17328
6.1 %
13573
4.8 %
2490
0.9 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2020)
England - HRA
Extended Flexible IFN-GAMMA test
30
2201
0.8%
96
4.4%
2025
92.0%
97
4.4 %
74
3.4 %
6
0.3 %
Parallel New Infection
75
9622
3.4%
439
4.6%
8808
91.5%
483
5.0 %
371
3.9 %
4
0.0 %
Parallel - new infection cull area
911 104838
36.9%
4596
4.4%
94577
90.2%
6495
6.2 %
5022
4.8 %
643
0.6 %
Parallel - Other
6
487
0.2%
80
16.4%
384
78.9%
26
5.3 %
23
4.7 %
0
Parallel Persistent Infection
51
8552
3.0%
415
4.9%
7799
91.2%
436
5.1 %
332
3.9 %
6
0.1 %
Parallel - persistent infection cull area
55
9410
3.3%
599
6.4%
8351
88.7%
573
6.1 %
440
4.7 %
20
0.2 %
Serial - Anomalous Reaction
3
25
0.0%
0
0.0 %
25
100.0%
0
0
0
England - Edge
Parallel New Infection
663
80268
28.2%
2476
3.1%
72563
90.4%
5104
6.4 %
4005
5.0 %
1224
1.5 %
Parallel - new infection cull area
10
1332
0.5%
29
2.2%
1261
94.7%
73
5.5 %
40
3.0 %
2
0.2 %
Parallel Persistent Infection
7
816
0.3%
22
2.7%
769
94.2%
34
4.2 %
25
3.1 %
0
England - LRA
Parallel New Infection
60
4148
1.5%
103
2.5%
3462
83.5%
246
5.9 %
208
5.0 %
375
9.0 %
Parallel Persistent Infection
3
67
0.0%
1
1.5%
59
88.1%
9
13.4 %
7
10.4 %
0
England
xx-xxxx-xxxx
78.0%
8856
4.0%
200083
90.2%
13576
6.1 %
10547
4.8 %
2280
1.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
71
1891
0.7%
160
8.5%
1659
87.7%
98
5.2 %
65
3.4 %
7
0.4 %
Parallel - Herd or Group Slaughter
44
1300
0.5%
75
5.8%
1179
90.7%
67
5.2 %
46
3.5 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2020) Cont.
Parallel New Infection
9
164
0.1%
7
4.3%
153
93.3%
4
2.4 %
4
2.4 %
0
Parallel - Other
250
5972
2.1%
586
9.8%
5086
85.2%
359
6.0 %
292
4.9 %
8
0.1 %
Parallel Persistent Infection
410
22169
7.8%
1169
5.3%
19886
89.7%
1283
5.8 %
1046
4.7 %
68
0.3 %
Parallel - Rapid Testing Two-times IRs
389
1477
0.5%
287
19.4%
1125
76.2%
83
5.6 %
62
4.2 %
3
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
18
520
0.2%
39
7.5%
448
86.2%
41
7.9 %
31
6.0 %
2
0.4 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
127
12385
4.4%
373
3.0%
11345
91.6%
774
6.2 %
652
5.3 %
15
0.1 %
Parallel - Other
44
793
0.3%
58
7.3%
690
87.0%
56
7.1 %
45
5.7 %
0
Parallel Persistent Infection
58
3182
1.1%
200
6.3%
2852
89.6%
153
4.8 %
125
3.9 %
5
0.2 %
Parallel - Rapid Testing Two-times IRs
61
167
0.1%
43
25.7%
120
71.9%
4
2.4 %
4
2.4 %
0
Wales - Low TB
Parallel - Herd or Group Slaughter
4
155
0.1%
1
0.6%
147
94.8%
8
5.2 %
7
4.5 %
0
Parallel New Infection
73
6100
2.1%
112
1.8%
5630
92.3%
417
6.8 %
321
5.3 %
37
0.6 %
Parallel - Other
6
23
0.0%
1
4.3%
16
69.6%
6
26.1 %
6
26.1 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
7
34
0.0%
1
2.9%
30
88.2%
3
8.8 %
3
8.8 %
0
Wales
1575
56402
19.8%
3117
5.5%
50422
89.4%
3369
6.0 %
2718
4.8 %
145
0.3 %
Scotland - Scotland
Parallel New Infection
50
5221
1.8%
115
2.2%
4821
92.3%
264
5.1 %
220
4.2 %
65
1.2 %
Parallel - Other
7
782
0.3%
15
1.9%
700
89.5%
94
12.0 %
67
8.6 %
0
Parallel Persistent Infection
4
249
0.1%
27
10.8%
203
81.5%
23
9.2 %
19
7.6 %
0
Serial - Suspected NSR Herd
2
27
0.0%
4
14.8%
21
77.8%
2
7.4 %
2
7.4 %
0
Scotland
63
6279
2.2%
161
2.6%
5745
91.5%
383
6.1 %
308
4.9 %
65
1.0 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 January
343
31274
227
1367
1
7
0
0
0
0
178
3
0
8
0
1110
1
57
0
0
February
323
33146
192
1289
1
21
0
0
0
0
187
3
0
6
0
982
0
89
0
0
March
262
18221
147
764
1
9
0
0
0
0
100
4
0
4
0
609
0
37
0
0
April
184
10928
96
408
2
6
0
0
0
0
52
0
0
3
0
309
0
35
0
1
May
227
17571
127
858
1
6
0
0
0
0
67
2
0
4
0
715
0
62
0
0
June
244
23217
149
860
5
0
0
0
0
53
0
0
4
0
742
1
55
0
0
July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
1
70
0
0
August
290
24676
173
888
15
0
0
0
0
145
0
0
3
1
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
0
0
4
2
617
3
63
0
0
October
316
26668
189
1198
14
0
0
0
0
114
0
4
1
3
987
44
30
0
0
November
347
26584
200
1359
46
0
0
0
0
103
0
11
0
0
1114
84
0
0
0
December
324
24146
186
1228
218
617
0
0
0
0
14
0
1
0
0
254
0
0
0
0
Totals (last
12 months)
3512
284447
2041
12134
224
778
0
0
0
0
1286
12
16
45
6
8925
134
578
0
1
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1874
221766
1201
8856
644
0
0
0
0
1154
7
10
15
4
6693
6
42
0
0
Scotland
63
6279
34
161
29
0
0
0
0
16
0
1
5
0
82
7
17
0
0
Wales
1575
56402
806
3117
105
0
0
0
0
116
5
5
25
2
2150
121
519
0
1
Totals
3512
284447
2041
12134
778
0
0
0
0
1286
12
16
45
6
8925
134
578
0
1
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
0
50
100
150
200
250
300
350
400
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2019
2020
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2019
2020
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
2019
2020
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
Wiltshire
Warks
Staffs
Shropshire
Powys
Oxon
Notts
NE Wales
Hampshire
Gwent
Gloucs
Dumfries & G
Devon
Corn & Scilly
Ceredigion
Berks
Argyll & Bute
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during December 2020 (% submission per
County)
County
%Resampled %Retested
Argyll & Bute
8.37 %
10.13 %
Avon
Berks
3.98 %
4.59 %
Carms
13.86 %
13.86 %
Ceredigion
3.13 %
6.25 %
Cheshire
2.71 %
3.35 %
Corn & Scilly
4.86 %
5.79 %
Derbyshire
8.37 %
9.02 %
Devon
6.87 %
8.04 %
Dorset
3.18 %
3.89 %
Dumfries & G
3.35 %
3.77 %
E Sussex
8.36 %
8.73 %
Gloucs
7.11 %
8.19 %
Gr Manchester
Gwent
2.63 %
2.63 %
Gwynedd
12.50 %
12.50 %
Hampshire
18.18 %
18.18 %
Leics & Rut
7.54 %
8.04 %
NE Wales
6.93 %
7.58 %
Northants
7.77 %
8.55 %
Notts
13.40 %
13.40 %
N Yorks
4.78 %
5.46 %
Oxon
5.26 %
5.73 %
Pembs
7.06 %
8.36 %
Powys
4.51 %
5.00 %
S Glamorgan
Shropshire
5.33 %
5.64 %
Somerset excl
4.52 %
4.59 %
Staffs
6.28 %
7.32 %
S Wales
Warks
5.76 %
5.76 %
Wigtown
25.00 %
25.00 %
Wiltshire
3.01 %
3.99 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19680
1005
5.1%
19851
1005
5.1%
Wales
3721
193
5.2%
3825
193
5.0%
Scotland
453
30
6.6%
470
30
6.4%
GB
23853
1228
5.1%
24146
1228
5.1%
Table 6a (i): Number of animals and submissions tested in December 2020
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
209635
8856
4.2%
221766
8856
4.0%
Wales
51713
3116
6.0%
56402
3117
5.5%
Scotland
5354
161
3.0%
6279
161
2.6%
GB
266699
12133
4.5%
284447
12134
4.3%
Table 6b (i): Number of animals and submissions tested in 2020 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
18769
623
3.3%
19595
623
3.2%
Wales
5491
281
5.1%
5597
281
5.0%
GB
24260
904
3.7%
25192
904
3.6%
Table 6a (ii): Number of animals and submissions tested in December 2019
Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of December
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
230030
10026
4.4%
263774
10026
3.8%
Wales
59269
3198
5.4%
65499
3198
4.9%
Scotland
2015
90
4.5%
2693
90
3.3%
GB
291304
13314
4.6%
331966
13314
4.0%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
2
5
0
0
Wiltshire
PRV-SP
1
1
0
0
Total
3
6
0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples December 2020
Table 7b Number of private samples 2020
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SE
1
12
1
8.33 %
PRV-SP
3
3
0
0
Cumbria
PRV-SP
1
1
0
0
Devon
PRV-SE
3
289
8
2.77 %
PRV-SP
3
11
0
0
Dorset
PRV-SE
1
1
0
0
PRV-SP
3
5
0
0
East Sussex
PRV-SP
1
1
0
0
Essex
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Hampshire
PRV-SP
1
3
0
0
Northern Somerset & South Glou
PRV-SE
1
39
1
2.56 %
Oxfordshire
PRV-SP
1
32
0
0
Somerset excl North
PRV-SE
1
28
0
0
PRV-SP
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Wiltshire
PRV-SE
2
280
15
5.36 %
PRV-SP
1
1
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
29
745
28
3.76 %
Isle of Man
SER-IOM
19
186
0
0

Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Carmarthenshire
2
10
1
10.0%
Ceredigion
2
9
3
33.3%
Devon
1
52
1
1.9%
North East Wales
6
108
13
12.0%
Pembrokeshire
23
250
32
12.8%
Powys
8
76
14
18.4%
South Wales
1
5
1
20.0%
Staffordshire
1
39
4
10.3%
TC0077
44
549
69
12.6 %
Table 8a Number of Cattle Antibody Tests December 2020
Test
County
Num Submissions
Num Samples Tested
Num Positive Samples % Positive
TC0077
Berkshire
2
315
11
3.5%
Carmarthenshire
56
830
149
18.0%
Ceredigion
44
684
102
14.9%
Cornwall and Isles of
Scilly
1
85
3
3.5%
Devon
7
1,550
125
8.1%
North East Wales
51
1,370
136
9.9%
Pembrokeshire
157
2,311
329
14.2%
Powys
52
666
92
13.8%
South Wales
30
392
70
17.9%
Staffordshire
2
61
5
8.2%
Wiltshire
2
581
8
1.4%
TC0077
404
8,845
1,030
11.6 %
Table 8b Number of Cattle Antibody Tests 2020