Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - April 2023
Source: environment.data.gov.uk (2024-07-03)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2023. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.

Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 200
Report Period 1st - 30th April 2023
Issued 10 May 2023

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
April 2023
%
Total 2023
%
Submissions
203
1015
Samples
Total (%)
16194
100.00 %
77322
100.00 %
England
8681
53.61 %
49611
64.16 %
Wales
5263
32.50 %
22688
29.34 %
Scotland
2250
13.89 %
5023
6.50 %
Parallel Tests
PAR-CHR
1025
6.33 %
1025
1.33 %
PAR-CUL-N
488
3.01 %
2318
3.00 %
PAR-CUL-P
0
0.00 %
539
0.70 %
PAR-HERD-S
0
0.00 %
396
0.51 %
PAR-IR-EX
33
0.20 %
172
0.22 %
PAR-IR-SEV
151
0.93 %
750
0.97 %
PAR-NEW-IN
5766
35.61 %
29480
38.13 %
PAR-OTHER
139
0.86 %
1476
1.91 %
PAR-PERSIS
1372
8.47 %
4890
6.32 %
PAR-POSTM
0
0.00 %
94
0.12 %
PAR-RAPID
66
0.41 %
300
0.39 %
PAR-RECUR
5904
36.46 %
32645
42.22 %
Total(% of all samples)
14944
92.28 %
74085
95.81 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
1236
7.63 %
3223
4.17 %
SER-FRAUD
14
0.09 %
14
0.02 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
Total(% of all samples)
1250
7.72 %
3237
4.19 %
Total
16194
100.00 %
77322
100.00 %
Retests
Total (% of all samples)
1117
6.90 %
6076
7.86 %
England
547
3.38 %
3698
4.78 %
Wales
359
2.22 %
1890
2.44 %
Scotland
211
1.30 %
488
0.63 %
Resamples
Total (% of all samples)
931
5.75 %
5045
6.52 %
England
470
2.90 %
3178
4.11 %
Wales
265
1.64 %
1414
1.83 %
Scotland
196
1.21 %
453
0.59 %
Rejects
Total (% of all samples)
38
0.23 %
1535
1.99 %
England
9
0.06 %
1110
1.44 %
Wales
29
0.18 %
351
0.45 %
Scotland
0
0.00 %
74
0.10 %
Table 1. Monthly Totals for April 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CHR
PAR-CUL-N
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-FRAUD
Breakdown of
Reason for
submission
PAR-CHR
1025
6.3 %
PAR-CUL-N
488
3.0 %
PAR-IR-EX
33
0.2 %
PAR-IR-SEV
151
0.9 %
PAR-NEW-IN
5766
35.6 %
PAR-OTHER
139
0.9 %
PAR-PERSIS
1372
8.5 %
PAR-RAPID
66
0.4 %
PAR-RECUR
5904
36.5 %
SER-FLEXI
1236
7.6 %
SER-FRAUD
14
0.1 %
Total
16194
100.00 %
Figure 1. Number of samples submitted in each category during April 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
500
1000
1500
2000
2500
3000
Kirkcudbright
Ayrshire
W Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Cheshire
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Humberside
Heref
Hampshire
Gloucs
E Sussex
Devon
Derbyshire
Cumbria
Corn & Scilly
Bucks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-N
PAR-CHR
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during April 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
Wigtown
West lothian
Kirkcudbright
Ayrshire
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NW Wales
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Cheshire
Ceredigion
Carms
W Yorks
Wiltshire
Warks
Surrey
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FRAUD
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
1
160
0.99 %
11
6.88 %
138
86.25 %
11
6.88 %
11
6.88 %
0
0.00 %
Corn & Scilly
PAR-PERSIS
3
460
2.84 %
39
8.48 %
407
88.48 %
19
4.13 %
14
3.04 %
0
0.00 %
PAR-RECUR
7
1,011
6.24 %
47
4.65 %
917
90.70 %
64
6.33 %
47
4.65 %
0
0.00 %
Devon
PAR-CUL-N
2
460
2.84 %
19
4.13 %
422
91.74 %
14
3.04 %
14
3.04 %
5
1.09 %
PAR-RECUR
2
42
0.26 %
2
4.76 %
33
78.57 %
8
19.05 %
7
16.67 %
0
0.00 %
Gloucs
PAR-CUL-N
1
19
0.12 %
3
15.79 %
16
84.21 %
0
0.00 %
0
0.00 %
0
0.00 %
Heref
PAR-OTHER
1
17
0.10 %
1
5.88 %
12
70.59 %
4
23.53 %
4
23.53 %
0
0.00 %
PAR-RECUR
1
7
0.04 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-RECUR
3
609
3.76 %
11
1.81 %
560
91.95 %
42
6.90 %
38
6.24 %
0
0.00 %
Somerset excl N
PAR-OTHER
1
1
0.01 %
1
100.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
739
4.56 %
40
5.41 %
677
91.61 %
27
3.65 %
22
2.98 %
0
0.00 %
Staffs
PAR-CHR
3
1,025
6.33 %
42
4.10 %
876
85.46 %
117
11.41 %
106
10.34 %
1
0.10 %
PAR-RECUR
6
1,660
10.25 %
30
1.81 %
1569
94.52 %
68
4.10 %
61
3.67 %
0
0.00 %
Wiltshire
PAR-RECUR
2
235
1.45 %
13
5.53 %
211
89.79 %
10
4.26 %
9
3.83 %
2
0.85 %
England - Edge
Bucks
PAR-NEW-IN
2
24
0.15 %
3
12.50 %
20
83.33 %
1
4.17 %
1
4.17 %
0
0.00 %
PAR-RECUR
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Derbyshire
PAR-NEW-IN
2
185
1.14 %
18
9.73 %
163
88.11 %
8
4.32 %
4
2.16 %
0
0.00 %
PAR-RECUR
1
296
1.83 %
4
1.35 %
280
94.59 %
14
4.73 %
12
4.05 %
0
0.00 %
E Sussex
PAR-NEW-IN
3
229
1.41 %
3
1.31 %
226
98.69 %
2
0.87 %
0
0.00 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
36
0.22 %
1
2.78 %
33
91.67 %
3
8.33 %
2
5.56 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
24
0.15 %
0
0.00 %
15
62.50 %
9
37.50 %
9
37.50 %
0
0.00 %
PAR-RECUR
1
135
0.83 %
11
8.15 %
120
88.89 %
7
5.19 %
4
2.96 %
0
0.00 %
Northants
PAR-NEW-IN
2
145
0.90 %
6
4.14 %
128
88.28 %
12
8.28 %
11
7.59 %
0
0.00 %
PAR-RECUR
2
408
2.52 %
6
1.47 %
374
91.67 %
32
7.84 %
28
6.86 %
0
0.00 %
Oxon
PAR-NEW-IN
1
16
0.10 %
0
0.00 %
12
75.00 %
4
25.00 %
4
25.00 %
0
0.00 %
SER-FLEXI
2
55
0.34 %
3
5.45 %
48
87.27 %
4
7.27 %
4
7.27 %
0
0.00 %
Table 2a. Summary by County for April 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Warks
PAR-NEW-IN
1
21
0.13 %
0
0.00 %
19
90.48 %
4
19.05 %
2
9.52 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
2
544
3.36 %
10
1.84 %
496
91.18 %
40
7.35 %
38
6.99 %
0
0.00 %
Humberside
PAR-NEW-IN
1
62
0.38 %
0
0.00 %
56
90.32 %
9
14.52 %
5
8.06 %
1
1.61 %
Lincs
PAR-NEW-IN
1
55
0.34 %
1
1.82 %
42
76.36 %
12
21.82 %
12
21.82 %
0
0.00 %
England
63
8681
53.61 %
325
3.74 %
7877
90.74 %
547
6.30 %
470
5.41 %
9
0.10 %
Wales - High TB
Carms
PAR-IR-SEV
3
9
0.06 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
76
0.47 %
1
1.32 %
70
92.11 %
5
6.58 %
5
6.58 %
0
0.00 %
PAR-RAPID
8
27
0.17 %
6
22.22 %
21
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
203
1.25 %
17
8.37 %
185
91.13 %
2
0.99 %
1
0.49 %
0
0.00 %
SER-FLEXI
5
219
1.35 %
18
8.22 %
191
87.21 %
13
5.94 %
10
4.57 %
0
0.00 %
SER-FRAUD
1
14
0.09 %
0
0.00 %
13
92.86 %
1
7.14 %
1
7.14 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
11
0.07 %
1
9.09 %
8
72.73 %
2
18.18 %
2
18.18 %
0
0.00 %
PAR-PERSIS
2
614
3.79 %
28
4.56 %
570
92.83 %
20
3.26 %
14
2.28 %
2
0.33 %
PAR-RAPID
2
6
0.04 %
0
0.00 %
6
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
1
35
0.22 %
2
5.71 %
33
94.29 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
1
6
0.04 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
252
1.56 %
18
7.14 %
228
90.48 %
8
3.17 %
5
1.98 %
1
0.40 %
SER-FLEXI
8
609
3.76 %
45
7.39 %
528
86.70 %
41
6.73 %
32
5.25 %
4
0.66 %
Pembs
PAR-IR-EX
5
23
0.14 %
8
34.78 %
15
65.22 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
18
103
0.64 %
32
31.07 %
69
66.99 %
3
2.91 %
2
1.94 %
0
0.00 %
PAR-PERSIS
4
298
1.84 %
11
3.69 %
262
87.92 %
30
10.07 %
23
7.72 %
2
0.67 %
PAR-RAPID
10
18
0.11 %
6
33.33 %
12
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
16
0.10 %
1
6.25 %
7
43.75 %
8
50.00 %
8
50.00 %
0
0.00 %
SER-FLEXI
2
318
1.96 %
26
8.18 %
279
87.74 %
26
8.18 %
13
4.09 %
0
0.00 %
Powys
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
9
0.06 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
3
37
0.23 %
5
13.51 %
19
51.35 %
13
35.14 %
12
32.43 %
1
2.70 %
W Glamorgan
PAR-OTHER
1
45
0.28 %
0
0.00 %
44
97.78 %
3
6.67 %
0
0.00 %
1
2.22 %
Wales - Intermediate TB
Carms
PAR-RAPID
3
8
0.05 %
4
50.00 %
4
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
93
0.57 %
19
20.43 %
71
76.34 %
6
6.45 %
3
3.23 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
12
0.07 %
0
0.00 %
9
75.00 %
5
41.67 %
3
25.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
13
0.08 %
4
30.77 %
9
69.23 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
10
892
5.51 %
35
3.92 %
808
90.58 %
54
6.05 %
45
5.04 %
4
0.45 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-IR-SEV
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
1
147
0.91 %
6
4.08 %
137
93.20 %
5
3.40 %
4
2.72 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
9
708
4.37 %
17
2.40 %
630
88.98 %
83
11.72 %
57
8.05 %
4
0.56 %
NE Wales
PAR-IR-SEV
1
4
0.02 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
425
2.62 %
11
2.59 %
379
89.18 %
31
7.29 %
25
5.88 %
10
2.35 %
Wales
128
5263
32.50 %
331
6.29 %
4638
88.12 %
359
6.82 %
265
5.04 %
29
0.55 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
3
484
2.99 %
9
1.86 %
443
91.53 %
36
7.44 %
32
6.61 %
0
0.00 %
Kirkcudbright
PAR-CUL-N
1
9
0.06 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
8
1,757
10.85 %
117
6.66 %
1477
84.06 %
174
9.90 %
163
9.28 %
0
0.00 %
Scotland
12
2250
13.89 %
126
5.60 %
1928
85.69 %
211
9.38 %
196
8.71 %
0
0.00 %
Grand Total
Sum:
203
16194
782
14443
1117
931
38
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
8
458
0.59 %
23
5.02 %
406
88.65 %
30
6.55 %
28
6.11 %
1
0.22 %
Corn & Scilly
PAR-CUL-N
3
926
1.20 %
60
6.48 %
804
86.83 %
80
8.64 %
62
6.70 %
0
0.00 %
PAR-CUL-P
1
539
0.70 %
31
5.75 %
481
89.24 %
32
5.94 %
26
4.82 %
1
0.19 %
PAR-OTHER
3
324
0.42 %
24
7.41 %
271
83.64 %
33
10.19 %
29
8.95 %
0
0.00 %
PAR-PERSIS
4
541
0.70 %
42
7.76 %
461
85.21 %
43
7.95 %
38
7.02 %
0
0.00 %
PAR-RECUR
27
6,994
9.05 %
294
4.20 %
6201
88.66 %
560
8.01 %
498
7.12 %
1
0.01 %
Devon
PAR-CUL-N
7
1,173
1.52 %
65
5.54 %
1043
88.92 %
68
5.80 %
60
5.12 %
5
0.43 %
PAR-HERD-S
2
396
0.51 %
35
8.84 %
326
82.32 %
48
12.12 %
35
8.84 %
0
0.00 %
PAR-RECUR
26
4,854
6.28 %
341
7.03 %
3993
82.26 %
320
6.59 %
253
5.21 %
267
5.50 %
Dorset
PAR-RECUR
2
717
0.93 %
12
1.67 %
321
44.77 %
22
3.07 %
22
3.07 %
362
50.49 %
Gloucs
PAR-CUL-N
1
19
0.02 %
3
15.79 %
16
84.21 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
541
0.70 %
25
4.62 %
479
88.54 %
43
7.95 %
37
6.84 %
0
0.00 %
Heref
PAR-OTHER
2
83
0.11 %
3
3.61 %
74
89.16 %
6
7.23 %
6
7.23 %
0
0.00 %
PAR-RECUR
15
3,059
3.96 %
178
5.82 %
2666
87.15 %
247
8.07 %
215
7.03 %
0
0.00 %
Shropshire
PAR-RECUR
29
2,536
3.28 %
111
4.38 %
2217
87.42 %
254
10.02 %
206
8.12 %
2
0.08 %
Somerset excl N
PAR-OTHER
2
12
0.02 %
2
16.67 %
9
75.00 %
2
16.67 %
1
8.33 %
0
0.00 %
PAR-PERSIS
2
296
0.38 %
47
15.88 %
226
76.35 %
25
8.45 %
23
7.77 %
0
0.00 %
PAR-RECUR
14
1,532
1.98 %
70
4.57 %
1400
91.38 %
70
4.57 %
59
3.85 %
3
0.20 %
Staffs
PAR-CHR
3
1,025
1.33 %
42
4.10 %
876
85.46 %
117
11.41 %
106
10.34 %
1
0.10 %
PAR-OTHER
1
38
0.05 %
1
2.63 %
37
97.37 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
24
4,625
5.98 %
92
1.99 %
4154
89.82 %
381
8.24 %
349
7.55 %
30
0.65 %
Wiltshire
PAR-RECUR
9
1,139
1.47 %
54
4.74 %
1043
91.57 %
56
4.92 %
38
3.34 %
4
0.35 %
England - Edge
Berks
PAR-NEW-IN
4
784
1.01 %
10
1.28 %
726
92.60 %
50
6.38 %
48
6.12 %
0
0.00 %
PAR-RECUR
1
132
0.17 %
2
1.52 %
118
89.39 %
13
9.85 %
11
8.33 %
1
0.76 %
Bucks
PAR-CUL-N
1
191
0.25 %
3
1.57 %
182
95.29 %
6
3.14 %
6
3.14 %
0
0.00 %
PAR-NEW-IN
13
1,411
1.82 %
38
2.69 %
1318
93.41 %
57
4.04 %
54
3.83 %
1
0.07 %
Table 2b. Summary by County for 2023
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
1
0.00 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Cheshire
PAR-OTHER
3
516
0.67 %
16
3.10 %
489
94.77 %
11
2.13 %
11
2.13 %
0
0.00 %
PAR-PERSIS
8
1,187
1.54 %
32
2.70 %
1040
87.62 %
127
10.70 %
115
9.69 %
0
0.00 %
PAR-RECUR
2
215
0.28 %
2
0.93 %
201
93.49 %
15
6.98 %
12
5.58 %
0
0.00 %
Derbyshire
PAR-NEW-IN
7
616
0.80 %
35
5.68 %
539
87.50 %
52
8.44 %
42
6.82 %
0
0.00 %
PAR-RECUR
6
1,306
1.69 %
36
2.76 %
1185
90.74 %
93
7.12 %
85
6.51 %
0
0.00 %
E Sussex
PAR-NEW-IN
11
834
1.08 %
18
2.16 %
770
92.33 %
58
6.95 %
46
5.52 %
0
0.00 %
Hampshire
PAR-NEW-IN
6
799
1.03 %
32
4.01 %
724
90.61 %
52
6.51 %
41
5.13 %
2
0.25 %
Leics & Rut
PAR-NEW-IN
14
1,908
2.47 %
56
2.94 %
1769
92.71 %
99
5.19 %
83
4.35 %
0
0.00 %
PAR-RECUR
1
135
0.17 %
11
8.15 %
120
88.89 %
7
5.19 %
4
2.96 %
0
0.00 %
Northants
PAR-NEW-IN
8
931
1.20 %
23
2.47 %
726
77.98 %
75
8.06 %
64
6.87 %
118
12.67 %
PAR-RECUR
2
408
0.53 %
6
1.47 %
374
91.67 %
32
7.84 %
28
6.86 %
0
0.00 %
Notts
PAR-NEW-IN
7
301
0.39 %
10
3.32 %
270
89.70 %
37
12.29 %
19
6.31 %
2
0.66 %
Oxon
PAR-NEW-IN
2
193
0.25 %
4
2.07 %
172
89.12 %
21
10.88 %
17
8.81 %
0
0.00 %
PAR-PERSIS
1
235
0.30 %
14
5.96 %
205
87.23 %
16
6.81 %
16
6.81 %
0
0.00 %
PAR-RECUR
5
373
0.48 %
28
7.51 %
314
84.18 %
36
9.65 %
31
8.31 %
0
0.00 %
SER-FLEXI
2
55
0.07 %
3
5.45 %
48
87.27 %
4
7.27 %
4
7.27 %
0
0.00 %
Warks
PAR-NEW-IN
7
683
0.88 %
22
3.22 %
638
93.41 %
34
4.98 %
23
3.37 %
0
0.00 %
PAR-OTHER
1
19
0.02 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
19
100.00 %
PAR-RECUR
10
152
0.20 %
5
3.29 %
116
76.32 %
19
12.50 %
17
11.18 %
14
9.21 %
England - LRA
Cumbria
PAR-NEW-IN
10
2,260
2.92 %
65
2.88 %
2011
88.98 %
207
9.16 %
184
8.14 %
0
0.00 %
Essex
PAR-NEW-IN
4
353
0.46 %
1
0.28 %
173
49.01 %
4
1.13 %
2
0.57 %
177
50.14 %
Gr Manchester
PAR-PERSIS
1
41
0.05 %
0
0.00 %
41
100.00 %
1
2.44 %
0
0.00 %
0
0.00 %
Humberside
PAR-NEW-IN
1
62
0.08 %
0
0.00 %
56
90.32 %
9
14.52 %
5
8.06 %
1
1.61 %
Lancashire
PAR-NEW-IN
1
75
0.10 %
2
2.67 %
72
96.00 %
2
2.67 %
1
1.33 %
0
0.00 %
Lincs
PAR-NEW-IN
8
950
1.23 %
18
1.89 %
851
89.58 %
85
8.95 %
81
8.53 %
0
0.00 %
N Yorks
PAR-NEW-IN
2
82
0.11 %
1
1.22 %
78
95.12 %
4
4.88 %
3
3.66 %
0
0.00 %
Surrey
PAR-NEW-IN
4
507
0.66 %
22
4.34 %
358
70.61 %
29
5.72 %
29
5.72 %
98
19.33 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
W Yorks
PAR-NEW-IN
1
69
0.09 %
0
0.00 %
65
94.20 %
5
7.25 %
4
5.80 %
0
0.00 %
England
346
49611
64.16 %
2070
4.17 %
43253
87.18 %
3698
7.45 %
3178
6.41 %
1110
2.24 %
Wales - High TB
Carms
PAR-IR-EX
5
17
0.02 %
8
47.06 %
9
52.94 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
17
111
0.14 %
43
38.74 %
61
54.95 %
7
6.31 %
7
6.31 %
0
0.00 %
PAR-OTHER
1
76
0.10 %
1
1.32 %
70
92.11 %
5
6.58 %
5
6.58 %
0
0.00 %
PAR-PERSIS
6
126
0.16 %
0
0.00 %
110
87.30 %
14
11.11 %
12
9.52 %
4
3.17 %
PAR-RAPID
26
75
0.10 %
23
30.67 %
47
62.67 %
5
6.67 %
4
5.33 %
1
1.33 %
PAR-RECUR
6
446
0.58 %
36
8.07 %
395
88.57 %
16
3.59 %
14
3.14 %
1
0.22 %
SER-FLEXI
10
690
0.89 %
50
7.25 %
605
87.68 %
41
5.94 %
32
4.64 %
3
0.43 %
SER-FRAUD
1
14
0.02 %
0
0.00 %
13
92.86 %
1
7.14 %
1
7.14 %
0
0.00 %
Ceredigion
PAR-IR-EX
5
16
0.02 %
2
12.50 %
12
75.00 %
2
12.50 %
2
12.50 %
0
0.00 %
PAR-IR-SEV
7
21
0.03 %
7
33.33 %
11
52.38 %
3
14.29 %
3
14.29 %
0
0.00 %
PAR-PERSIS
2
614
0.79 %
28
4.56 %
570
92.83 %
20
3.26 %
14
2.28 %
2
0.33 %
PAR-RAPID
6
13
0.02 %
4
30.77 %
8
61.54 %
1
7.69 %
1
7.69 %
0
0.00 %
PAR-RECUR
3
147
0.19 %
6
4.08 %
137
93.20 %
3
2.04 %
3
2.04 %
1
0.68 %
SER-FLEXI
9
490
0.63 %
86
17.55 %
380
77.55 %
37
7.55 %
24
4.90 %
0
0.00 %
Gwent
PAR-IR-EX
2
14
0.02 %
9
64.29 %
5
35.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
16
0.02 %
9
56.25 %
6
37.50 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-RAPID
6
15
0.02 %
3
20.00 %
12
80.00 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
382
0.49 %
22
5.76 %
349
91.36 %
18
4.71 %
10
2.62 %
1
0.26 %
SER-FLEXI
9
719
0.93 %
49
6.82 %
632
87.90 %
45
6.26 %
34
4.73 %
4
0.56 %
Pembs
PAR-IR-EX
24
81
0.10 %
34
41.98 %
43
53.09 %
3
3.70 %
3
3.70 %
1
1.23 %
PAR-IR-SEV
63
408
0.53 %
142
34.80 %
238
58.33 %
34
8.33 %
17
4.17 %
11
2.70 %
PAR-OTHER
1
14
0.02 %
5
35.71 %
8
57.14 %
1
7.14 %
1
7.14 %
0
0.00 %
PAR-PERSIS
19
1,365
1.77 %
112
8.21 %
1144
83.81 %
131
9.60 %
104
7.62 %
5
0.37 %
PAR-RAPID
38
116
0.15 %
30
25.86 %
79
68.10 %
9
7.76 %
6
5.17 %
1
0.86 %
PAR-RECUR
15
1,221
1.58 %
66
5.41 %
1050
86.00 %
99
8.11 %
80
6.55 %
25
2.05 %
SER-FLEXI
14
1,018
1.32 %
68
6.68 %
898
88.21 %
77
7.56 %
51
5.01 %
1
0.10 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-IR-EX
5
8
0.01 %
0
0.00 %
6
75.00 %
2
25.00 %
2
25.00 %
0
0.00 %
PAR-IR-SEV
14
97
0.13 %
14
14.43 %
73
75.26 %
13
13.40 %
9
9.28 %
1
1.03 %
PAR-NEW-IN
2
152
0.20 %
13
8.55 %
131
86.18 %
10
6.58 %
8
5.26 %
0
0.00 %
PAR-PERSIS
3
4
0.01 %
0
0.00 %
2
50.00 %
1
25.00 %
1
25.00 %
1
25.00 %
PAR-POSTM
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
15
27
0.03 %
3
11.11 %
24
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
8
307
0.40 %
14
4.56 %
236
76.87 %
59
19.22 %
54
17.59 %
3
0.98 %
S Wales
PAR-IR-SEV
1
9
0.01 %
6
66.67 %
3
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
3
120
0.16 %
6
5.00 %
98
81.67 %
19
15.83 %
15
12.50 %
1
0.83 %
Wales - Intermediate TB
Carms
PAR-IR-SEV
1
4
0.01 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
253
0.33 %
43
17.00 %
195
77.08 %
15
5.93 %
14
5.53 %
1
0.40 %
PAR-RAPID
4
9
0.01 %
4
44.44 %
5
55.56 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
490
0.63 %
23
4.69 %
439
89.59 %
27
5.51 %
23
4.69 %
5
1.02 %
Ceredigion
PAR-IR-EX
2
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
341
0.44 %
45
13.20 %
271
79.47 %
29
8.50 %
25
7.33 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
12
0.02 %
0
0.00 %
9
75.00 %
5
41.67 %
3
25.00 %
0
0.00 %
Mid Glamorgan
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
NE Wales
PAR-IR-EX
10
30
0.04 %
7
23.33 %
21
70.00 %
2
6.67 %
2
6.67 %
0
0.00 %
PAR-IR-SEV
8
73
0.09 %
14
19.18 %
58
79.45 %
1
1.37 %
1
1.37 %
0
0.00 %
PAR-NEW-IN
37
3,080
3.98 %
83
2.69 %
2765
89.77 %
241
7.82 %
212
6.88 %
20
0.65 %
PAR-OTHER
1
9
0.01 %
2
22.22 %
7
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
6
128
0.17 %
7
5.47 %
106
82.81 %
18
14.06 %
15
11.72 %
0
0.00 %
PAR-POSTM
12
65
0.08 %
6
9.23 %
56
86.15 %
4
6.15 %
3
4.62 %
0
0.00 %
PAR-RAPID
13
28
0.04 %
6
21.43 %
21
75.00 %
1
3.57 %
1
3.57 %
0
0.00 %
PAR-RECUR
1
56
0.07 %
1
1.79 %
50
89.29 %
6
10.71 %
5
8.93 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
4
86
0.11 %
6
6.98 %
75
87.21 %
5
5.81 %
5
5.81 %
0
0.00 %
Powys
PAR-IR-EX
1
2
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
239
0.31 %
9
3.77 %
215
89.96 %
17
7.11 %
15
6.28 %
0
0.00 %
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
128
0.17 %
0
0.00 %
118
92.19 %
13
10.16 %
10
7.81 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Shropshire
PAR-PERSIS
2
5
0.01 %
0
0.00 %
5
100.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-IR-SEV
2
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
61
4,632
5.99 %
133
2.87 %
4173
90.09 %
376
8.12 %
305
6.58 %
21
0.45 %
PAR-OTHER
2
11
0.01 %
1
9.09 %
7
63.64 %
3
27.27 %
3
27.27 %
0
0.00 %
PAR-PERSIS
1
7
0.01 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
PAR-POSTM
5
5
0.01 %
2
40.00 %
3
60.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
291
0.38 %
10
3.44 %
267
91.75 %
18
6.19 %
13
4.47 %
1
0.34 %
NE Wales
PAR-IR-EX
1
1
0.00 %
1
100.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
5
0.01 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
40
3,509
4.54 %
81
2.31 %
2970
84.64 %
407
11.60 %
222
6.33 %
236
6.73 %
PAR-POSTM
9
23
0.03 %
3
13.04 %
20
86.96 %
1
4.35 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
4
165
0.21 %
12
7.27 %
138
83.64 %
17
10.30 %
15
9.09 %
0
0.00 %
NW Wales
PAR-NEW-IN
2
24
0.03 %
0
0.00 %
23
95.83 %
1
4.17 %
1
4.17 %
0
0.00 %
Wales
622
22688
29.34 %
1411
6.22 %
19512
86.00 %
1890
8.33 %
1414
6.23 %
351
1.55 %
Scotland - Scotland
Ayrshire
PAR-NEW-IN
10
1,252
1.62 %
84
6.71 %
1035
82.67 %
144
11.50 %
128
10.22 %
5
0.40 %
Kirkcudbright
PAR-CUL-N
1
9
0.01 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-NEW-IN
8
1,757
2.27 %
117
6.66 %
1477
84.06 %
174
9.90 %
163
9.28 %
0
0.00 %
West lothian
PAR-NEW-IN
9
797
1.03 %
31
3.89 %
640
80.30 %
61
7.65 %
58
7.28 %
68
8.53 %
Wigtown
PAR-NEW-IN
19
1,208
1.56 %
110
9.11 %
994
82.28 %
108
8.94 %
103
8.53 %
1
0.08 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2023 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Scotland
47
5023
6.50 %
342
6.81 %
4154
82.70 %
488
9.72 %
453
9.02 %
74
1.47 %
Grand Total
Sum:
1015
77322
3823
66919
6076
5045
1535
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol April 2023
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
61
8626
99.4%
322
3.7%
7829
90.8%
543
6.3%
466
5.4%
9
0.1%
Serial/Extended
2
55
0.6%
3
5.5%
48
87.3%
4
7.3%
4
7.3%
0
0.0 %
Total
63
8681
100.0%
325
3.7%
7877
90.7%
547
6.3%
470
5.4%
9
0.1%
Scotland
Parallel
12
2250
100.0%
126
5.6%
1928
85.7%
211
9.4%
196
8.7%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
12
2250
100.0%
126
5.6%
1928
85.7%
211
9.4%
196
8.7%
0
0.0 %
Wales
Parallel
111
4068
77.3%
240
5.9%
3594
88.3%
278
6.8%
209
5.1%
25
0.6%
Serial/Extended
17
1195
22.7%
91
7.6%
1044
87.4%
81
6.8%
56
4.7%
4
0.3%
Total
128
5263
100.0%
331
6.3%
4638
88.1%
359
6.8%
265
5.0%
29
0.6%
GB
Parallel
184
14944
92.3%
688
4.6%
13351
89.3%
1032
6.9%
871
5.8%
34
0.2%
Serial/Extended
19
1250
7.7%
94
7.5%
1092
87.4%
85
6.8%
60
4.8%
4
0.3%
Total
203
16194
100.0%
782
4.8%
14443
89.2%
1117
6.9%
931
5.7%
38
0.2%
63.00
128.00
12.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2023)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
344
49556
99.9%
2067
4.2%
43205
87.2%
3694
7.5%
3174
6.4%
1110
2.2%
Serial/Extended
2
55
0.1%
3
5.5%
48
87.3%
4
7.3%
4
7.3%
0
0.0 %
Total
346
49611
100.0%
2070
4.2%
43253
87.2%
3698
7.5%
3178
6.4%
1110
2.2%
Scotland
Parallel
47
5023
100.0%
342
6.8%
4154
82.7%
488
9.7%
453
9.0%
74
1.5%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
47
5023
100.0%
342
6.8%
4154
82.7%
488
9.7%
453
9.0%
74
1.5%
Wales
Parallel
571
19506
86.0%
1140
5.8%
16771
86.0%
1667
8.5%
1252
6.4%
343
1.8%
Serial/Extended
51
3182
14.0%
271
8.5%
2741
86.1%
223
7.0%
162
5.1%
8
0.3%
Total
622
22688
100.0%
1411
6.2%
19512
86.0%
1890
8.3%
1414
6.2%
351
1.5%
GB
Parallel
962
74085
95.8%
3549
4.8%
64130
86.6%
5849
7.9%
4879
6.6%
1527
2.1%
Serial/Extended
53
3237
4.2%
274
8.5%
2789
86.2%
227
7.0%
166
5.1%
8
0.2%
Total
1015
77322
100.0%
3823
4.9%
66919
86.5%
6076
7.9%
5045
6.5%
1535
2.0%
346.00
622.00
47.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
18
1236
7.6%
94
7.6%
1079
87.3%
84
6.8 %
59
4.8 %
4
0.3 %
Parallel – chronic breakdown
3
1025
6.3%
42
4.1%
876
85.5%
117
11.4 %
106
10.3 %
1
0.1 %
Parallel - IRs in Exit Strategy for persistent her
10
33
0.2%
13
39.4%
20
60.6%
0
0
0
Parallel New Infection
57
5766
35.6%
237
4.1%
5093
88.3%
492
8.5 %
417
7.2 %
19
0.3 %
Parallel - new infection cull area
4
488
3.0%
22
4.5%
446
91.4%
15
3.1 %
15
3.1 %
5
1.0 %
Parallel - Other
4
139
0.9%
3
2.2%
126
90.6%
13
9.4 %
9
6.5 %
1
0.7 %
Parallel Persistent Infection
9
1372
8.5%
78
5.7%
1239
90.3%
69
5.0 %
51
3.7 %
4
0.3 %
Parallel - Rapid Testing Two-times IRs
28
66
0.4%
16
24.2%
50
75.8%
0
0
0
Parallel - Recurrent breakdowns
41
5904
36.5%
235
4.0%
5396
91.4%
321
5.4 %
269
4.6 %
4
0.1 %
Parallel - severe IRs - in persistent herds
28
151
0.9%
42
27.8%
105
69.5%
5
3.3 %
4
2.6 %
0
Serial - Anomalous Reaction
1
14
0.1%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Total
203
16194
100.0%
782
4.8%
14443
89.2%
1117
6.9 %
931
5.7 %
38
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (April 2023)
England - HRA
Parallel – chronic breakdown
3
1025
6.3%
42
4.1%
876
85.5%
117
11.4 %
106
10.3 %
1
0.1 %
Parallel - new infection cull area
3
479
3.0%
22
4.6%
438
91.4%
14
2.9 %
14
2.9 %
5
1.0 %
Parallel - Other
2
18
0.1%
2
11.1%
12
66.7%
5
27.8 %
4
22.2 %
0
Parallel Persistent Infection
3
460
2.8%
39
8.5%
407
88.5%
19
4.1 %
14
3.0 %
0
Parallel - Recurrent breakdowns
27
4463
27.6%
154
3.5%
4112
92.1%
230
5.2 %
195
4.4 %
2
0.0 %
England - HRA
38
6445
39.8%
259
4.0%
5845
90.7%
385
6.0 %
333
5.2 %
8
0.1 %
England - Edge
Extended Flexible IFN-GAMMA test
2
55
0.3%
3
5.5%
48
87.3%
4
7.3 %
4
7.3 %
0
Parallel New Infection
14
680
4.2%
31
4.6%
616
90.6%
43
6.3 %
33
4.9 %
0
Parallel - Recurrent breakdowns
5
840
5.2%
21
2.5%
774
92.1%
54
6.4 %
45
5.4 %
0
England - Edge
21
1575
9.7%
55
3.5%
1438
91.3%
101
6.4 %
82
5.2 %
0
England - LRA
Parallel New Infection
4
661
4.1%
11
1.7%
594
89.9%
61
9.2 %
55
8.3 %
1
0.2 %
England - LRA
4
661
4.1%
11
1.7%
594
89.9%
61
9.2 %
55
8.3 %
1
0.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
16
1181
7.3%
91
7.7%
1031
87.3%
80
6.8 %
55
4.7 %
4
0.3 %
Parallel - IRs in Exit Strategy for persistent her
8
31
0.2%
13
41.9%
18
58.1%
0
0
0
Parallel - Other
2
121
0.7%
1
0.8%
114
94.2%
8
6.6 %
5
4.1 %
1
0.8 %
Parallel Persistent Infection
6
912
5.6%
39
4.3%
832
91.2%
50
5.5 %
37
4.1 %
4
0.4 %
Parallel - Rapid Testing Two-times IRs
23
56
0.3%
12
21.4%
44
78.6%
0
0
0
Parallel - Recurrent breakdowns
8
508
3.1%
41
8.1%
439
86.4%
31
6.1 %
26
5.1 %
2
0.4 %
Parallel - severe IRs - in persistent herds
24
132
0.8%
34
25.8%
94
71.2%
5
3.8 %
4
3.0 %
0
Serial - Anomalous Reaction
1
14
0.1%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Wales - High TB
88
2955
18.2%
231
7.8%
2585
87.5%
175
5.9 %
128
4.3 %
11
0.4 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel New Infection
13
1051
6.5%
41
3.9%
954
90.8%
64
6.1 %
52
4.9 %
4
0.4 %
Parallel - Rapid Testing Two-times IRs
5
10
0.1%
4
40.0%
6
60.0%
0
0
0
Parallel - Recurrent breakdowns
1
93
0.6%
19
20.4%
71
76.3%
6
6.5 %
3
3.2 %
0
Parallel - severe IRs - in persistent herds
3
15
0.1%
5
33.3%
10
66.7%
0
0
0
Wales - Intermediate TB
24
1171
7.2%
69
5.9%
1043
89.1%
70
6.0 %
55
4.7 %
4
0.3 %
Wales - Low TB
Parallel New Infection
15
1133
7.0%
28
2.5%
1009
89.1%
114
10.1 %
82
7.2 %
14
1.2 %
Parallel - severe IRs - in persistent herds
1
4
0.0%
3
75.0%
1
25.0%
0
0
0
Wales - Low TB
16
1137
7.0%
31
2.7%
1010
88.8%
114
10.0 %
82
7.2 %
14
1.2 %
Scotland - Scotland
Parallel New Infection
11
2241
13.8%
126
5.6%
1920
85.7%
210
9.4 %
195
8.7 %
0
Parallel - new infection cull area
1
9
0.1%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Scotland - Scotland
12
2250
13.9%
126
5.6%
1928
85.7%
211
9.4 %
196
8.7 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
52
3223
4.2%
274
8.5%
2776
86.1%
226
7.0 %
165
5.1 %
8
0.2 %
Parallel – chronic breakdown
3
1025
1.3%
42
4.1%
876
85.5%
117
11.4 %
106
10.3 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
396
0.5%
35
8.8%
326
82.3%
48
12.1 %
35
8.8 %
0
Parallel - IRs in Exit Strategy for persistent herds
55
172
0.2%
63
36.6%
99
57.6%
10
5.8 %
9
5.2 %
1
0.6 %
Parallel New Infection
303
29480
38.1%
1018
3.5%
25748
87.3%
2424
8.2 %
1964
6.7 %
750
2.5 %
Parallel - new infection cull area
13
2318
3.0%
131
5.7%
2053
88.6%
155
6.7 %
129
5.6 %
5
0.2 %
Parallel - Other
24
1476
1.9%
104
7.0%
1266
85.8%
95
6.4 %
85
5.8 %
21
1.4 %
Parallel Persistent Infection
57
4890
6.3%
327
6.7%
4187
85.6%
427
8.7 %
364
7.4 %
12
0.2 %
Parallel - persistent infection cull area
1
539
0.7%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel – post-mvt test coupled with IDEXX anti
27
94
0.1%
11
11.7%
80
85.1%
5
5.3 %
3
3.2 %
0
Parallel - Rapid Testing Two-times IRs
118
300
0.4%
75
25.0%
209
69.7%
19
6.3 %
14
4.7 %
2
0.7 %
Parallel - Recurrent breakdowns
238
32645
42.2%
1468
4.5%
28349
86.8%
2458
7.5 %
2106
6.5 %
722
2.2 %
Parallel - severe IRs - in persistent herds
121
750
1.0%
244
32.5%
456
60.8%
59
7.9 %
38
5.1 %
12
1.6 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Total
1015
77322
100.0%
3823
4.9%
66919
86.5%
6076
7.9 %
5045
6.5 %
1535
2.0 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2023)
England - HRA
Parallel – chronic breakdown
3
1025
1.3%
42
4.1%
876
85.5%
117
11.4 %
106
10.3 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
396
0.5%
35
8.8%
326
82.3%
48
12.1 %
35
8.8 %
0
Parallel - new infection cull area
11
2118
2.7%
128
6.0%
1863
88.0%
148
7.0 %
122
5.8 %
5
0.2 %
Parallel - Other
8
457
0.6%
30
6.6%
391
85.6%
41
9.0 %
36
7.9 %
0
Parallel Persistent Infection
6
837
1.1%
89
10.6%
687
82.1%
68
8.1 %
61
7.3 %
0
Parallel - persistent infection cull area
1
539
0.7%
31
5.8%
481
89.2%
32
5.9 %
26
4.8 %
1
0.2 %
Parallel - Recurrent breakdowns
160
26455
34.2%
1200
4.5%
22880
86.5%
1983
7.5 %
1705
6.4 %
670
2.5 %
England - Edge
Extended Flexible IFN-GAMMA test
2
55
0.1%
3
5.5%
48
87.3%
4
7.3 %
4
7.3 %
0
Parallel New Infection
79
8460
10.9%
248
2.9%
7652
90.4%
535
6.3 %
437
5.2 %
123
1.5 %
Parallel - new infection cull area
1
191
0.2%
3
1.6%
182
95.3%
6
3.1 %
6
3.1 %
0
Parallel - Other
4
535
0.7%
16
3.0%
489
91.4%
11
2.1 %
11
2.1 %
19
3.6 %
Parallel Persistent Infection
9
1422
1.8%
46
3.2%
1245
87.6%
143
10.1 %
131
9.2 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Parallel - Recurrent breakdowns
28
2722
3.5%
90
3.3%
2428
89.2%
216
7.9 %
189
6.9 %
15
0.6 %
England - LRA
Parallel New Infection
31
4358
5.6%
109
2.5%
3664
84.1%
345
7.9 %
309
7.1 %
276
6.3 %
Parallel Persistent Infection
1
41
0.1%
0
0.0 %
41
100.0%
1
2.4 %
0
0
England
346
49611
64.2%
2070
4.2%
43253
87.2%
3698
7.5 %
3178
6.4 %
1110
2.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
42
2917
3.8%
253
8.7%
2515
86.2%
200
6.9 %
141
4.8 %
8
0.3 %
Parallel - IRs in Exit Strategy for persistent herd
41
136
0.2%
53
39.0%
75
55.1%
7
5.1 %
7
5.1 %
1
0.7 %
Parallel New Infection
2
152
0.2%
13
8.6%
131
86.2%
10
6.6 %
8
5.3 %
0
Parallel - Other
5
210
0.3%
12
5.7%
176
83.8%
25
11.9 %
21
10.0 %
1
0.5 %
Parallel Persistent Infection
30
2109
2.7%
140
6.6%
1826
86.6%
166
7.9 %
131
6.2 %
12
0.6 %
Parallel – post-mvt test coupled with IDEXX ant
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
92
247
0.3%
64
25.9%
170
68.8%
16
6.5 %
11
4.5 %
2
0.8 %
Parallel - Recurrent breakdowns
35
2503
3.2%
144
5.8%
2167
86.6%
195
7.8 %
161
6.4 %
31
1.2 %
Parallel - severe IRs - in persistent herds
104
662
0.9%
221
33.4%
392
59.2%
58
8.8 %
37
5.6 %
12
1.8 %
Serial - Anomalous Reaction
1
14
0.0%
0
0.0 %
13
92.9%
1
7.1 %
1
7.1 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
86
0.1%
6
7.0%
75
87.2%
5
5.8 %
5
5.8 %
0
Parallel - IRs in Exit Strategy for persistent herd
13
35
0.0%
9
25.7%
24
68.6%
2
5.7 %
2
5.7 %
0
Parallel New Infection
42
3331
4.3%
92
2.8%
2989
89.7%
263
7.9 %
230
6.9 %
20
0.6 %
Parallel - Other
5
263
0.3%
45
17.1%
203
77.2%
15
5.7 %
14
5.3 %
1
0.4 %
Parallel Persistent Infection
10
474
0.6%
52
11.0%
382
80.6%
48
10.1 %
40
8.4 %
0
Parallel – post-mvt test coupled with IDEXX ant
12
65
0.1%
6
9.2%
56
86.2%
4
6.2 %
3
4.6 %
0
Parallel - Rapid Testing Two-times IRs
21
43
0.1%
11
25.6%
30
69.8%
2
4.7 %
2
4.7 %
0
Parallel - Recurrent breakdowns
10
674
0.9%
24
3.6%
607
90.1%
46
6.8 %
38
5.6 %
5
0.7 %
Parallel - severe IRs - in persistent herds
13
81
0.1%
19
23.5%
61
75.3%
1
1.2 %
1
1.2 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
4
165
0.2%
12
7.3%
138
83.6%
17
10.3 %
15
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
1
1
0.0%
1
100.0%
0
0.0 %
1
100.0 %
0
0
Parallel New Infection
103
8165
10.6%
214
2.6%
7166
87.8%
784
9.6 %
528
6.5 %
257
3.1 %
Parallel - Other
2
11
0.0%
1
9.1%
7
63.6%
3
27.3 %
3
27.3 %
0
Parallel Persistent Infection
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Parallel – post-mvt test coupled with IDEXX ant
14
28
0.0%
5
17.9%
23
82.1%
1
3.6 %
0
0
Parallel - Rapid Testing Two-times IRs
5
10
0.0%
0
0.0 %
9
90.0%
1
10.0 %
1
10.0 %
0
Parallel - Recurrent breakdowns
5
291
0.4%
10
3.4%
267
91.8%
18
6.2 %
13
4.5 %
1
0.3 %
Parallel - severe IRs - in persistent herds
4
7
0.0%
4
57.1%
3
42.9%
0
0
0
Wales
622
22688
29.3%
1411
6.2%
19512
86.0%
1890
8.3 %
1414
6.2 %
351
1.5 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2023) Cont.
Scotland - Scotland
Parallel New Infection
46
5014
6.5%
342
6.8%
4146
82.7%
487
9.7 %
452
9.0 %
74
1.5 %
Parallel - new infection cull area
1
9
0.0%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Scotland
47
5023
6.5%
342
6.8%
4154
82.7%
488
9.7 %
453
9.0 %
74
1.5 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2022 May
269
23949
158
918
2
19
0
0
0
0
62
3
0
9
0
778
0
44
1
0
June
213
17755
114
590
19
0
0
0
0
48
1
0
2
0
460
1
57
2
0
July
248
15402
109
485
4
0
0
0
0
46
1
2
3
0
382
1
46
0
0
August
274
19758
144
758
12
0
0
0
0
36
3
0
14
0
642
0
47
1
2
September
205
xx-xxxx-xxxx
107
672
13
0
0
0
0
100
0
1
7
0
520
0
31
0
0
October
180
xx-xxxx-xxxx
100
620
12
0
0
0
0
66
0
0
21
0
495
0
26
0
0
November
201
15848
120
673
16
0
0
0
0
60
1
1
13
0
504
1
77
0
0
December
200
13735
109
545
37
0
0
0
0
26
1
0
10
0
426
0
41
0
0
2023 January
281
23696
176
1138
11
0
0
0
0
83
1
0
17
2
921
1
98
2
2
February
265
17538
147
1036
10
0
0
0
0
69
0
1
26
5
837
0
68
0
20
March
266
19894
159
867
38
0
0
0
0
75
0
1
0
10
681
30
1
0
31
April
203
16194
118
782
318
214
0
0
0
0
6
0
0
0
1
37
0
0
0
0
Totals (last
12 months)
2805
216609
1561
9084
320
405
0
0
0
0
677
11
6
122
18
6683
34
536
6
55
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1017
144901
697
5193
254
0
0
0
0
558
2
3
24
3
4158
1
12
1
0
Scotland
79
8975
55
456
4
0
0
0
0
93
1
2
24
4
180
2
19
0
1
Wales
1709
62733
809
3435
147
0
0
0
0
26
8
1
74
11
2345
31
505
5
54
Totals
2805
216609
1561
9084
405
0
0
0
0
677
11
6
122
18
6683
34
536
6
55
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
134
195
100
138
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2021
2022
2023
IFNg Positive herds
IFNg Negative herds
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
24089
1349
12181
759
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2021
2022
2023
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2021
2022
2023
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
10
20
30
40
50
60
70
Wiltshire
Warks
Somerset excl N
Powys
Oxon
NE Wales
Lincs
Kirkcudbright
Heref
Gwynedd
Gloucs
Devon
Cumbria
Cheshire
Carms
Ayrshire
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during April 2023 (% submission per County)
County
%Resampled %Retested
Avon
6.88 %
6.88 %
Ayrshire
6.61 %
7.44 %
Bucks
8.00 %
8.00 %
Carms
3.08 %
4.16 %
Ceredigion
2.39 %
3.29 %
Cheshire
25.00 %
41.67 %
Corn & Scilly
4.15 %
5.64 %
Cumbria
6.99 %
7.35 %
Derbyshire
3.33 %
4.57 %
Devon
4.18 %
4.38 %
E Sussex
0.87 %
Gloucs
Gwent
4.27 %
5.65 %
Gwynedd
8.05 %
11.72 %
Hampshire
5.56 %
8.33 %
Heref
16.67 %
16.67 %
Humberside
8.06 %
14.52 %
Kirkcudbright
9.29 %
9.91 %
Leics & Rut
8.18 %
10.06 %
Lincs
21.82 %
21.82 %
Mid Glamorgan
NE Wales
5.24 %
6.36 %
Northants
7.05 %
7.96 %
Oxon
11.27 %
11.27 %
Pembs
5.93 %
8.63 %
Powys
8.00 %
9.00 %
Shropshire
6.24 %
6.90 %
Somerset excl
2.97 %
3.78 %
Staffs
6.22 %
6.89 %
Warks
9.52 %
19.05 %
W Glamorgan
6.67 %
Wiltshire
3.83 %
4.26 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
8642
325
3.8%
8681
325
3.7%
Wales
5220
331
6.3%
5263
331
6.3%
Scotland
2166
126
5.8%
2250
126
5.6%
GB
16028
782
4.9%
16194
782
4.8%
Table 6a (i): Number of animals and submissions tested in April 2023
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
47033
2070
4.4%
49611
2070
4.2%
Wales
21376
1411
6.6%
22688
1411
6.2%
Scotland
3491
342
9.8%
5023
342
6.8%
GB
71896
3823
5.3%
77322
3823
4.9%
Table 6b (i): Number of animals and submissions tested in 2023 Up to end of April
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
9730
391
4.0%
9857
391
4.0%
Wales
4634
284
6.1%
4738
284
6.0%
Scotland
472
12
2.5%
472
12
2.5%
GB
14836
687
4.6%
15067
687
4.6%
Table 6a (ii): Number of animals and submissions tested in April 2022
Table 6b (ii): Number of animals and submissions tested in 2022 Up to end of April
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
56151
2427
4.3%
58596
2427
4.1%
Wales
15019
1174
7.8%
15801
1174
7.4%
Scotland
477
12
2.5%
477
12
2.5%
GB
71647
3613
5.0%
74874
3613
4.8%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
Devon
PRV-SE
1
3
0
0
West Sussex
PRV-SE
2
524
24
4.58 %
Total
4
528
24
4.55 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples April 2023
Table 7b Number of private samples 2023
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
2
3
0
0
Cumbria
PRV-SE
1
3
3
100.00 %
Devon
PRV-SE
5
102
10
9.80 %
Oxfordshire
PRV-SP
2
2
0
0
Shropshire
PRV-SP
2
2
0
0
West Sussex
PRV-SE
3
525
24
4.57 %
PRV-SP
1
5
0
0
Total
16
642
37
5.76 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
Wales
Carmarthenshire
BOV-IR-C
1
4
1
25.0%
BOV-OTHER
1
34
9
26.5%
Ceredigion
BOV-IR-C
3
41
0
0
BOV-IR-EX
1
10
3
30.0%
North East Wales
BOV-IR-C
4
34
10
29.4%
Pembrokeshire
BOV-IR-C
18
133
26
19.5%
BOV-IR-EX
2
10
3
30.0%
BOV-OTHER
1
18
1
5.6%
BOV-P
1
28
1
3.6%
Powys
BOV-IR-C
3
4
0
0
South Wales
BOV-IR-EX
1
6
4
66.7%
BOV-P
1
62
1
1.6%
Wales
37
384
59
15.4%
All GB
37
384
59
15.4 %
Table 8a Number of Cattle Antibody Tests April 2023
Table 8b Number of Cattle Antibody Tests 2023
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
569
38
6.7%
Northern Somerset &
South
Gloucestershire
BOV-P
1
5
0
0
England
3
574
38
6.6%
Scotland
Ayrshire
BOV-OTHER
1
2
1
50.0%
Scotland
1
2
1
50.0%
Wales
Carmarthenshire
BOV-IR-C
12
115
31
27.0%
BOV-IR-EX
5
17
2
11.8%
BOV-OTHER
1
34
9
26.5%
BOV-P
1
49
1
2.0%
Ceredigion
BOV-IR-C
10
88
10
11.4%
BOV-IR-EX
2
13
3
23.1%
BOV-OTHER
1
80
2
2.5%
North East Wales
BOV-IR-C
9
71
19
26.8%
BOV-IR-EX
2
11
0
0
BOV-OTHER
1
54
0
0
BOV-P
1
14
0
0
BOV-POSTM
8
21
5
23.8%
Pembrokeshire
BOV-IR-C
51
478
78
16.3%
BOV-IR-EX
10
62
10
16.1%
BOV-OTHER
4
346
7
2.0%
BOV-P
1
28
1
3.6%
Powys
1
8
1
12.5%
BOV-IR-C
16
95
12
12.6%
South Wales
BOV-IR-C
3
26
6
23.1%
BOV-IR-EX
2
14
6
42.9%
BOV-OTHER
1
30
1
3.3%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-P
2
142
4
2.8%
Wales
144
1,796
208
11.6%
All GB
148
2,372
247
10.4 %