Monthly Report: Surveillance Project SB4008 IFNg (TC0651 and TC0751) & Antibody (TC0077) tests for bovine tuberculosis-April  2024
Source: environment.data.gov.uk (2025-04-29)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from January 2024. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month. While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports.
Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006).
Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number
Report Period
Issued 09 May 2024
212
1st April - 30th April 2024

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma (IFNG) blood testing
of skin test negative cattle to inform whole or
partial herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood IFNG testing of skin test-negative
cattle in persistently infected herds that have
failed to resolve by repeated short-interval skin
testing and fulfill a minimum of biosecurity
standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood IFNG testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel IFNG blood testing of all severe IRs in
the Exit Strategy for persistent herds (coupled
with Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel IFNG blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion in
badger control areas
England)
PAR-RECUR
England - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel IFNG blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-CHR
Parallel IFNG blood testing of Chronic
breakdown herds i.e. where VL reactors are
disclosed at a 3rd or subsequent Short Interval
test or a SLH case after a 2nd or subsequent
Short Interval test
England only - YES
PAR-POSTM
Post movement IFNG blood test of cattle
(coupled with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel IFNG blood testing not covered in
any of the other scenarios
NO (APHA discretion)
Test Criteria

'NSR' Herds (SER-NSR)
Modified serial IFNG blood testing of individual
skin test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial IFNG blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended IFNG blood test to provide flexible
test readout in a confirmed M. bovis-infected
herd where Johne's (M. a. paratuberculosis)
infection or vaccination is suspected to be
interferring with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with IFNG testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled IFNG testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with IFNG testing)
YES (Wales only)

Num
samples
April 2024
%
Total 2024
%
Submissions
237
943
Samples
Total (%)
18969
100.00 %
81350
100.00 %
England
11611
61.21 %
51987
63.91 %
Wales
7327
38.63 %
28494
35.03 %
Scotland
31
0.16 %
869
1.07 %
Parallel Tests
PAR-CHR
0
0.00 %
411
0.51 %
PAR-CUL-N
0
0.00 %
381
0.47 %
PAR-CUL-P
189
1.00 %
2614
3.21 %
PAR-HERD-S
0
0.00 %
44
0.05 %
PAR-IR-EX
19
0.10 %
131
0.16 %
PAR-IR-SEV
290
1.53 %
1182
1.45 %
PAR-NEW-IN
4771
25.15 %
16333
20.08 %
PAR-OTHER
866
4.57 %
2703
3.32 %
PAR-PERSIS
3891
20.51 %
16660
20.48 %
PAR-POSTM
0
0.00 %
PAR-RAPID
75
0.40 %
268
0.33 %
PAR-RECUR
8321
43.87 %
37888
46.57 %
Total(% of all samples)
18422
97.12 %
78615
96.64 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
SER-FLEXI
547
2.88 %
2467
3.03 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
SER-OTHER
0
0.00 %
268
0.33 %
Total(% of all samples)
547
2.88 %
2735
3.36 %
Total
18969
100.00 %
81350
100.00 %
Retests
Total (% of all samples)
1454
7.67 %
6483
7.97 %
England
943
4.97 %
4406
5.42 %
Wales
500
2.64 %
1970
2.42 %
Scotland
11
0.06 %
107
0.13 %
Resamples
Total (% of all samples)
1267
6.68 %
5648
6.94 %
England
831
4.38 %
3898
4.79 %
Wales
425
2.24 %
1657
2.04 %
Scotland
11
0.06 %
93
0.11 %
Rejects
Total (% of all samples)
45
0.24 %
1067
1.31 %
England
33
0.17 %
902
1.11 %
Wales
11
0.06 %
163
0.20 %
Scotland
1
0.01 %
2
0.00 %
Table 1. Monthly Totals for April 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPID
PAR-RECUR
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-P
189
1.0 %
PAR-IR-EX
19
0.1 %
PAR-IR-SEV
290
1.5 %
PAR-NEW-IN
4771
25.2 %
PAR-OTHER
866
4.6 %
PAR-PERSIS
3891
20.5 %
PAR-RAPID
75
0.4 %
PAR-RECUR
8321
43.9 %
SER-FLEXI
547
2.9 %
Total
18969
100.00 %
Figure 1. Number of samples submitted in each category during April 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or
subsequent SI test

0
200
400
600
800
1000
1200
1400
1600
1800
2000
Wigtown
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Conwy
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Notts
Northants
Leics & Rut
Humberside
Heref
Hampshire
Gloucs
E Sussex
Durham
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Avon
Scotland
Wales
England
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during April 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence,
PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in
confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown
herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test

0
2000
4000
6000
8000
10000
12000
Wigtown
West lothian
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Conwy
Ceredigion
Carms
W Sussex
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Norfolk
Lincs
Leics & Rut
Lancashire
Kent
Humberside
Herts
Heref
Hampshire
Gloucs
E Sussex
Durham
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
PAR-CHR
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER:
parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or
SLH case after a 2nd or subsequent SI test

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-RECUR
7
1,297
6.84 %
83
6.40 %
1166
89.90 %
60
4.63 %
46
3.55 %
2
0.15 %
Corn & Scilly
PAR-RECUR
1
214
1.13 %
17
7.94 %
180
84.11 %
18
8.41 %
17
7.94 %
0
0.00 %
Devon
PAR-CUL-P
2
189
1.00 %
8
4.23 %
82
43.39 %
105
55.56 %
99
52.38 %
0
0.00 %
PAR-OTHER
4
148
0.78 %
5
3.38 %
123
83.11 %
23
15.54 %
20
13.51 %
0
0.00 %
PAR-RECUR
13
1,409
7.43 %
97
6.88 %
1158
82.19 %
164
11.64 %
151
10.72 %
3
0.21 %
Dorset
PAR-RECUR
3
727
3.83 %
34
4.68 %
650
89.41 %
50
6.88 %
43
5.91 %
0
0.00 %
SER-FLEXI
2
106
0.56 %
6
5.66 %
97
91.51 %
4
3.77 %
3
2.83 %
0
0.00 %
Gloucs
PAR-RECUR
4
117
0.62 %
6
5.13 %
103
88.03 %
8
6.84 %
8
6.84 %
0
0.00 %
Heref
PAR-RECUR
6
544
2.87 %
18
3.31 %
464
85.29 %
66
12.13 %
62
11.40 %
0
0.00 %
Shropshire
PAR-OTHER
1
9
0.05 %
0
0.00 %
5
55.56 %
4
44.44 %
4
44.44 %
0
0.00 %
PAR-PERSIS
2
179
0.94 %
26
14.53 %
139
77.65 %
16
8.94 %
14
7.82 %
0
0.00 %
PAR-RECUR
9
1,218
6.42 %
36
2.96 %
1131
92.86 %
65
5.34 %
51
4.19 %
0
0.00 %
SER-FLEXI
1
347
1.83 %
73
21.04 %
260
74.93 %
17
4.90 %
14
4.03 %
0
0.00 %
Somerset excl N
PAR-OTHER
1
150
0.79 %
4
2.67 %
134
89.33 %
13
8.67 %
12
8.00 %
0
0.00 %
PAR-PERSIS
2
116
0.61 %
16
13.79 %
83
71.55 %
18
15.52 %
16
13.79 %
1
0.86 %
PAR-RECUR
2
431
2.27 %
26
6.03 %
350
81.21 %
56
12.99 %
55
12.76 %
0
0.00 %
Staffs
PAR-RECUR
3
291
1.53 %
18
6.19 %
257
88.32 %
16
5.50 %
16
5.50 %
0
0.00 %
Wiltshire
PAR-OTHER
1
470
2.48 %
13
2.77 %
442
94.04 %
23
4.89 %
15
3.19 %
0
0.00 %
PAR-RECUR
6
837
4.41 %
11
1.31 %
777
92.83 %
33
3.94 %
29
3.46 %
20
2.39 %
England - Edge
Bucks
PAR-NEW-IN
2
175
0.92 %
5
2.86 %
158
90.29 %
13
7.43 %
11
6.29 %
1
0.57 %
Cheshire
PAR-PERSIS
4
254
1.34 %
16
6.30 %
210
82.68 %
31
12.20 %
28
11.02 %
0
0.00 %
Derbyshire
PAR-NEW-IN
1
11
0.06 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
E Sussex
PAR-NEW-IN
1
353
1.86 %
16
4.53 %
324
91.78 %
20
5.67 %
13
3.68 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
12
0.06 %
0
0.00 %
11
91.67 %
1
8.33 %
1
8.33 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
6
981
5.17 %
50
5.10 %
873
88.99 %
71
7.24 %
58
5.91 %
0
0.00 %
Northants
PAR-NEW-IN
3
840
4.43 %
10
1.19 %
798
95.00 %
31
3.69 %
29
3.45 %
3
0.36 %
Table 2a. Summary by County for April 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Notts
PAR-NEW-IN
1
19
0.10 %
1
5.26 %
16
84.21 %
2
10.53 %
2
10.53 %
0
0.00 %
Oxon
PAR-NEW-IN
1
6
0.03 %
1
16.67 %
1
16.67 %
4
66.67 %
4
66.67 %
0
0.00 %
Warks
PAR-OTHER
1
55
0.29 %
15
27.27 %
39
70.91 %
1
1.82 %
1
1.82 %
0
0.00 %
England - LRA
Cumbria
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Durham
PAR-NEW-IN
2
99
0.52 %
3
3.03 %
86
86.87 %
8
8.08 %
7
7.07 %
3
3.03 %
Humberside
PAR-NEW-IN
1
6
0.03 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
England
95
11611
61.21 %
614
5.29 %
10133
87.27 %
943
8.12 %
831
7.16 %
33
0.28 %
Wales - High TB
Carms
PAR-IR-EX
3
6
0.03 %
3
50.00 %
2
33.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-IR-SEV
9
62
0.33 %
13
20.97 %
48
77.42 %
2
3.23 %
1
1.61 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
7
1,754
9.25 %
86
4.90 %
1613
91.96 %
62
3.53 %
51
2.91 %
4
0.23 %
PAR-RAPID
3
4
0.02 %
1
25.00 %
3
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
59
0.31 %
7
11.86 %
50
84.75 %
2
3.39 %
1
1.69 %
1
1.69 %
Ceredigion
PAR-IR-SEV
2
8
0.04 %
3
37.50 %
5
62.50 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
450
2.37 %
29
6.44 %
390
86.67 %
34
7.56 %
30
6.67 %
1
0.22 %
PAR-RECUR
1
77
0.41 %
9
11.69 %
68
88.31 %
1
1.30 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-SEV
1
21
0.11 %
9
42.86 %
12
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
30
0.16 %
2
6.67 %
25
83.33 %
4
13.33 %
3
10.00 %
0
0.00 %
PAR-PERSIS
1
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
17
0.09 %
8
47.06 %
8
47.06 %
2
11.76 %
1
5.88 %
0
0.00 %
PAR-RECUR
3
446
2.35 %
21
4.71 %
415
93.05 %
10
2.24 %
10
2.24 %
0
0.00 %
Pembs
PAR-IR-EX
3
7
0.04 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
17
132
0.70 %
48
36.36 %
72
54.55 %
13
9.85 %
12
9.09 %
0
0.00 %
PAR-OTHER
1
2
0.01 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-PERSIS
8
833
4.39 %
62
7.44 %
750
90.04 %
33
3.96 %
20
2.40 %
1
0.12 %
PAR-RAPID
11
39
0.21 %
15
38.46 %
19
48.72 %
5
12.82 %
5
12.82 %
0
0.00 %
PAR-RECUR
5
421
2.22 %
27
6.41 %
353
83.85 %
46
10.93 %
40
9.50 %
1
0.24 %
Powys
PAR-IR-SEV
5
10
0.05 %
0
0.00 %
9
90.00 %
1
10.00 %
1
10.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for April 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
2
296
1.56 %
18
6.08 %
267
90.20 %
13
4.39 %
11
3.72 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
S Wales
PAR-IR-SEV
1
17
0.09 %
4
23.53 %
13
76.47 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Ceredigion
PAR-RAPID
1
4
0.02 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
39
0.21 %
11
28.21 %
25
64.10 %
3
7.69 %
3
7.69 %
0
0.00 %
PAR-NEW-IN
6
354
1.87 %
22
6.21 %
315
88.98 %
20
5.65 %
17
4.80 %
0
0.00 %
PAR-PERSIS
1
4
0.02 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RAPID
4
5
0.03 %
1
20.00 %
4
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-RECUR
1
292
1.54 %
22
7.53 %
254
86.99 %
20
6.85 %
16
5.48 %
0
0.00 %
S Glamorgan
PAR-RAPID
1
5
0.03 %
2
40.00 %
3
60.00 %
1
20.00 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
1
28
0.15 %
0
0.00 %
28
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwynedd
PAR-IR-EX
1
3
0.02 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
13
1,171
6.17 %
23
1.96 %
1039
88.73 %
124
10.59 %
109
9.31 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
6
684
3.61 %
20
2.92 %
581
84.94 %
88
12.87 %
80
11.70 %
3
0.44 %
PAR-PERSIS
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
SER-FLEXI
1
35
0.18 %
4
11.43 %
22
62.86 %
11
31.43 %
9
25.71 %
0
0.00 %
Wales
141
7327
38.63 %
477
6.51 %
6414
87.54 %
500
6.82 %
425
5.80 %
11
0.15 %
Scotland - Scotland
Wigtown
PAR-NEW-IN
1
31
0.16 %
1
3.23 %
18
58.06 %
11
35.48 %
11
35.48 %
1
3.23 %
Scotland
1
31
0.16 %
1
3.23 %
18
58.06 %
11
35.48 %
11
35.48 %
1
3.23 %
Grand Total
Sum:
237
18969
1092
16565
1454
1267
45
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N:
new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CHR
3
333
0.41 %
47
14.11 %
264
79.28 %
24
7.21 %
22
6.61 %
0
0.00 %
PAR-PERSIS
5
756
0.93 %
91
12.04 %
621
82.14 %
48
6.35 %
44
5.82 %
0
0.00 %
PAR-RECUR
14
2,027
2.49 %
118
5.82 %
1804
89.00 %
128
6.31 %
103
5.08 %
2
0.10 %
Corn & Scilly
PAR-CUL-P
2
891
1.10 %
35
3.93 %
805
90.35 %
58
6.51 %
51
5.72 %
0
0.00 %
PAR-OTHER
3
947
1.16 %
98
10.35 %
766
80.89 %
97
10.24 %
83
8.76 %
0
0.00 %
PAR-PERSIS
11
1,491
1.83 %
124
8.32 %
1218
81.69 %
169
11.33 %
148
9.93 %
1
0.07 %
PAR-RECUR
29
2,450
3.01 %
196
8.00 %
1880
76.73 %
400
16.33 %
370
15.10 %
4
0.16 %
SER-FLEXI
4
45
0.06 %
12
26.67 %
30
66.67 %
2
4.44 %
2
4.44 %
1
2.22 %
Devon
PAR-CUL-N
4
239
0.29 %
13
5.44 %
212
88.70 %
17
7.11 %
14
5.86 %
0
0.00 %
PAR-CUL-P
4
197
0.24 %
8
4.06 %
88
44.67 %
107
54.31 %
101
51.27 %
0
0.00 %
PAR-OTHER
5
310
0.38 %
10
3.23 %
258
83.23 %
45
14.52 %
42
13.55 %
0
0.00 %
PAR-PERSIS
8
1,066
1.31 %
64
6.00 %
912
85.55 %
97
9.10 %
87
8.16 %
3
0.28 %
PAR-RECUR
32
3,960
4.87 %
216
5.45 %
3409
86.09 %
348
8.79 %
308
7.78 %
27
0.68 %
Dorset
PAR-RECUR
9
2,064
2.54 %
131
6.35 %
1833
88.81 %
111
5.38 %
98
4.75 %
2
0.10 %
SER-FLEXI
5
159
0.20 %
16
10.06 %
139
87.42 %
5
3.14 %
4
2.52 %
0
0.00 %
Gloucs
PAR-RECUR
18
2,307
2.84 %
130
5.64 %
1838
79.67 %
85
3.68 %
80
3.47 %
259
11.23 %
Heref
PAR-CUL-N
1
142
0.17 %
13
9.15 %
119
83.80 %
12
8.45 %
10
7.04 %
0
0.00 %
PAR-CUL-P
1
485
0.60 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
485
100.00 %
PAR-OTHER
1
150
0.18 %
10
6.67 %
131
87.33 %
11
7.33 %
9
6.00 %
0
0.00 %
PAR-PERSIS
1
80
0.10 %
1
1.25 %
77
96.25 %
2
2.50 %
2
2.50 %
0
0.00 %
PAR-RECUR
19
3,289
4.04 %
149
4.53 %
2862
87.02 %
313
9.52 %
277
8.42 %
1
0.03 %
Shropshire
PAR-CUL-P
2
357
0.44 %
39
10.92 %
308
86.27 %
10
2.80 %
10
2.80 %
0
0.00 %
PAR-OTHER
1
9
0.01 %
0
0.00 %
5
55.56 %
4
44.44 %
4
44.44 %
0
0.00 %
PAR-PERSIS
2
179
0.22 %
26
14.53 %
139
77.65 %
16
8.94 %
14
7.82 %
0
0.00 %
PAR-RECUR
26
3,927
4.83 %
189
4.81 %
3482
88.67 %
286
7.28 %
243
6.19 %
13
0.33 %
SER-FLEXI
3
414
0.51 %
86
20.77 %
301
72.71 %
35
8.45 %
27
6.52 %
0
0.00 %
Table 2b. Summary by County for 2024
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Somerset excl N
PAR-CHR
2
78
0.10 %
4
5.13 %
73
93.59 %
2
2.56 %
1
1.28 %
0
0.00 %
PAR-CUL-P
2
684
0.84 %
20
2.92 %
617
90.20 %
42
6.14 %
36
5.26 %
11
1.61 %
PAR-OTHER
1
150
0.18 %
4
2.67 %
134
89.33 %
13
8.67 %
12
8.00 %
0
0.00 %
PAR-PERSIS
2
116
0.14 %
16
13.79 %
83
71.55 %
18
15.52 %
16
13.79 %
1
0.86 %
PAR-RECUR
13
1,735
2.13 %
109
6.28 %
1505
86.74 %
126
7.26 %
118
6.80 %
3
0.17 %
Staffs
PAR-PERSIS
2
233
0.29 %
53
22.75 %
162
69.53 %
27
11.59 %
18
7.73 %
0
0.00 %
PAR-RECUR
17
1,526
1.88 %
60
3.93 %
1351
88.53 %
123
8.06 %
111
7.27 %
4
0.26 %
Wiltshire
PAR-OTHER
1
470
0.58 %
13
2.77 %
442
94.04 %
23
4.89 %
15
3.19 %
0
0.00 %
PAR-RECUR
19
3,678
4.52 %
112
3.05 %
3428
93.20 %
141
3.83 %
118
3.21 %
20
0.54 %
England - Edge
Berks
PAR-NEW-IN
4
386
0.47 %
25
6.48 %
345
89.38 %
17
4.40 %
13
3.37 %
3
0.78 %
Bucks
PAR-NEW-IN
5
215
0.26 %
7
3.26 %
192
89.30 %
17
7.91 %
15
6.98 %
1
0.47 %
PAR-RECUR
3
220
0.27 %
16
7.27 %
188
85.45 %
18
8.18 %
16
7.27 %
0
0.00 %
Cheshire
PAR-NEW-IN
2
1,061
1.30 %
32
3.02 %
792
74.65 %
287
27.05 %
237
22.34 %
0
0.00 %
PAR-OTHER
1
268
0.33 %
11
4.10 %
253
94.40 %
5
1.87 %
4
1.49 %
0
0.00 %
PAR-PERSIS
14
1,892
2.33 %
119
6.29 %
1570
82.98 %
229
12.10 %
203
10.73 %
0
0.00 %
Derbyshire
PAR-NEW-IN
1
11
0.01 %
0
0.00 %
11
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
668
0.82 %
17
2.54 %
610
91.32 %
45
6.74 %
39
5.84 %
2
0.30 %
E Sussex
PAR-NEW-IN
8
1,012
1.24 %
51
5.04 %
913
90.22 %
58
5.73 %
47
4.64 %
1
0.10 %
Hampshire
PAR-NEW-IN
1
12
0.01 %
0
0.00 %
11
91.67 %
1
8.33 %
1
8.33 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
19
2,730
3.36 %
98
3.59 %
2405
88.10 %
260
9.52 %
226
8.28 %
1
0.04 %
Northants
PAR-NEW-IN
14
1,593
1.96 %
52
3.26 %
1417
88.95 %
105
6.59 %
97
6.09 %
27
1.69 %
Notts
PAR-NEW-IN
6
436
0.54 %
12
2.75 %
369
84.63 %
58
13.30 %
55
12.61 %
0
0.00 %
Oxon
PAR-HERD-S
1
9
0.01 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-NEW-IN
3
367
0.45 %
23
6.27 %
303
82.56 %
43
11.72 %
41
11.17 %
0
0.00 %
PAR-RECUR
6
1,265
1.56 %
42
3.32 %
1156
91.38 %
74
5.85 %
63
4.98 %
4
0.32 %
Warks
PAR-OTHER
1
55
0.07 %
15
27.27 %
39
70.91 %
1
1.82 %
1
1.82 %
0
0.00 %
PAR-RECUR
5
627
0.77 %
26
4.15 %
513
81.82 %
73
11.64 %
88
14.04 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
2
33
0.04 %
2
6.06 %
9
27.27 %
5
15.15 %
5
15.15 %
17
51.52 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Cumbria
PAR-NEW-IN
6
612
0.75 %
18
2.94 %
552
90.20 %
44
7.19 %
41
6.70 %
1
0.16 %
SER-OTHER
1
7
0.01 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Durham
PAR-NEW-IN
2
99
0.12 %
3
3.03 %
86
86.87 %
8
8.08 %
7
7.07 %
3
3.03 %
Herts
PAR-NEW-IN
2
203
0.25 %
10
4.93 %
180
88.67 %
13
6.40 %
13
6.40 %
0
0.00 %
Humberside
PAR-NEW-IN
2
83
0.10 %
0
0.00 %
75
90.36 %
10
12.05 %
8
9.64 %
0
0.00 %
Kent
SER-OTHER
2
248
0.30 %
1
0.40 %
240
96.77 %
9
3.63 %
6
2.42 %
1
0.40 %
Lancashire
PAR-NEW-IN
2
103
0.13 %
1
0.97 %
98
95.15 %
5
4.85 %
3
2.91 %
1
0.97 %
Lincs
PAR-NEW-IN
2
221
0.27 %
3
1.36 %
207
93.67 %
12
5.43 %
11
4.98 %
0
0.00 %
Norfolk
PAR-NEW-IN
2
87
0.11 %
7
8.05 %
54
62.07 %
24
27.59 %
24
27.59 %
2
2.30 %
N Yorks
PAR-NEW-IN
4
507
0.62 %
7
1.38 %
465
91.72 %
38
7.50 %
34
6.71 %
1
0.20 %
W Sussex
SER-OTHER
2
13
0.02 %
0
0.00 %
13
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
England
401
51987
63.91 %
2811
5.41 %
44376
85.36 %
4406
8.48 %
3898
7.50 %
902
1.74 %
Wales - High TB
Carms
PAR-IR-EX
8
22
0.03 %
7
31.82 %
11
50.00 %
4
18.18 %
4
18.18 %
0
0.00 %
PAR-IR-SEV
29
211
0.26 %
79
37.44 %
123
58.29 %
10
4.74 %
7
3.32 %
2
0.95 %
PAR-OTHER
5
232
0.29 %
20
8.62 %
198
85.34 %
20
8.62 %
14
6.03 %
0
0.00 %
PAR-PERSIS
15
2,718
3.34 %
162
5.96 %
2454
90.29 %
116
4.27 %
97
3.57 %
5
0.18 %
PAR-RAPID
18
61
0.07 %
27
44.26 %
32
52.46 %
3
4.92 %
2
3.28 %
0
0.00 %
PAR-RECUR
6
2,576
3.17 %
46
1.79 %
2478
96.20 %
73
2.83 %
51
1.98 %
1
0.04 %
SER-FLEXI
3
59
0.07 %
7
11.86 %
50
84.75 %
2
3.39 %
1
1.69 %
1
1.69 %
Ceredigion
PAR-HERD-S
1
35
0.04 %
6
17.14 %
27
77.14 %
2
5.71 %
2
5.71 %
0
0.00 %
PAR-IR-EX
4
15
0.02 %
11
73.33 %
4
26.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
36
0.04 %
15
41.67 %
21
58.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
453
0.56 %
29
6.40 %
393
86.75 %
34
7.51 %
30
6.62 %
1
0.22 %
PAR-RAPID
4
7
0.01 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
77
0.09 %
9
11.69 %
68
88.31 %
1
1.30 %
0
0.00 %
0
0.00 %
Gwent
PAR-IR-EX
2
17
0.02 %
8
47.06 %
7
41.18 %
2
11.76 %
2
11.76 %
0
0.00 %
PAR-IR-SEV
4
29
0.04 %
11
37.93 %
18
62.07 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
4
30
0.04 %
2
6.67 %
25
83.33 %
4
13.33 %
3
10.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
3
76
0.09 %
8
10.53 %
67
88.16 %
1
1.32 %
1
1.32 %
0
0.00 %
PAR-RAPID
7
34
0.04 %
19
55.88 %
14
41.18 %
2
5.88 %
1
2.94 %
0
0.00 %
PAR-RECUR
6
610
0.75 %
25
4.10 %
564
92.46 %
23
3.77 %
21
3.44 %
0
0.00 %
Pembs
PAR-IR-EX
12
25
0.03 %
12
48.00 %
12
48.00 %
1
4.00 %
1
4.00 %
0
0.00 %
PAR-IR-SEV
73
705
0.87 %
259
36.74 %
394
55.89 %
58
8.23 %
52
7.38 %
0
0.00 %
PAR-OTHER
4
9
0.01 %
0
0.00 %
6
66.67 %
3
33.33 %
3
33.33 %
0
0.00 %
PAR-PERSIS
40
5,706
7.01 %
352
6.17 %
5020
87.98 %
294
5.15 %
219
3.84 %
115
2.02 %
PAR-RAPID
32
99
0.12 %
32
32.32 %
61
61.62 %
6
6.06 %
6
6.06 %
0
0.00 %
PAR-RECUR
27
2,727
3.35 %
120
4.40 %
2451
89.88 %
188
6.89 %
147
5.39 %
9
0.33 %
SER-FLEXI
11
1,313
1.61 %
75
5.71 %
1133
86.29 %
118
8.99 %
103
7.84 %
2
0.15 %
Powys
PAR-IR-EX
2
16
0.02 %
4
25.00 %
11
68.75 %
1
6.25 %
1
6.25 %
0
0.00 %
PAR-IR-SEV
12
70
0.09 %
28
40.00 %
39
55.71 %
3
4.29 %
3
4.29 %
0
0.00 %
PAR-OTHER
2
73
0.09 %
2
2.74 %
66
90.41 %
7
9.59 %
4
5.48 %
1
1.37 %
PAR-PERSIS
4
502
0.62 %
28
5.58 %
426
84.86 %
50
9.96 %
48
9.56 %
0
0.00 %
PAR-RAPID
4
7
0.01 %
4
57.14 %
3
42.86 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
14
1,037
1.27 %
60
5.79 %
931
89.78 %
56
5.40 %
44
4.24 %
2
0.19 %
SER-FLEXI
2
177
0.22 %
26
14.69 %
98
55.37 %
60
33.90 %
53
29.94 %
0
0.00 %
S Wales
PAR-IR-SEV
1
17
0.02 %
4
23.53 %
13
76.47 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
28
0.03 %
4
14.29 %
22
78.57 %
1
3.57 %
1
3.57 %
1
3.57 %
Wales - Intermediate TB
Carms
PAR-PERSIS
2
2
0.00 %
1
50.00 %
1
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
2
3
0.00 %
1
33.33 %
1
33.33 %
1
33.33 %
1
33.33 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
21
0.03 %
17
80.95 %
4
19.05 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
4
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-EX
6
11
0.01 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
15
75
0.09 %
22
29.33 %
46
61.33 %
7
9.33 %
7
9.33 %
0
0.00 %
PAR-NEW-IN
25
2,038
2.51 %
82
4.02 %
1799
88.27 %
171
8.39 %
147
7.21 %
10
0.49 %
PAR-PERSIS
19
1,331
1.64 %
65
4.88 %
1209
90.83 %
56
4.21 %
51
3.83 %
6
0.45 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2024 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
7
10
0.01 %
2
20.00 %
8
80.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
588
0.72 %
18
3.06 %
527
89.63 %
45
7.65 %
43
7.31 %
0
0.00 %
SER-FLEXI
2
128
0.16 %
21
xx-xxxx-xxxx %
100
78.13 %
8
6.25 %
7
5.47 %
0
0.00 %
Powys
PAR-IR-SEV
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
113
0.14 %
5
4.42 %
95
84.07 %
14
12.39 %
13
11.50 %
0
0.00 %
PAR-RAPID
2
18
0.02 %
4
22.22 %
11
61.11 %
3
16.67 %
3
16.67 %
0
0.00 %
PAR-RECUR
3
313
0.38 %
23
7.35 %
271
86.58 %
24
7.67 %
19
6.07 %
0
0.00 %
S Glamorgan
PAR-RAPID
3
14
0.02 %
4
28.57 %
10
71.43 %
1
7.14 %
0
0.00 %
0
0.00 %
Wales - Low TB
Conwy
PAR-NEW-IN
1
28
0.03 %
0
0.00 %
28
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwynedd
PAR-IR-EX
1
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
3
12
0.01 %
6
50.00 %
5
41.67 %
1
8.33 %
1
8.33 %
0
0.00 %
PAR-NEW-IN
28
1,862
2.29 %
47
2.52 %
1632
87.65 %
204
10.96 %
180
9.67 %
3
0.16 %
PAR-RAPID
2
2
0.00 %
0
0.00 %
1
50.00 %
1
50.00 %
1
50.00 %
0
0.00 %
PAR-RECUR
1
18
0.02 %
0
0.00 %
18
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
3
10
0.01 %
3
30.00 %
6
60.00 %
1
10.00 %
1
10.00 %
0
0.00 %
NE Wales
PAR-IR-EX
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
23
0.03 %
9
39.13 %
13
56.52 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-NEW-IN
13
1,652
2.03 %
62
3.75 %
1376
83.29 %
236
14.29 %
211
12.77 %
3
0.18 %
PAR-PERSIS
2
59
0.07 %
5
8.47 %
53
89.83 %
1
1.69 %
1
1.69 %
0
0.00 %
PAR-RAPID
2
9
0.01 %
3
33.33 %
4
44.44 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RECUR
1
171
0.21 %
3
1.75 %
148
86.55 %
19
11.11 %
19
11.11 %
1
0.58 %
SER-FLEXI
3
162
0.20 %
30
18.52 %
104
64.20 %
31
19.14 %
28
17.28 %
0
0.00 %
Wales
535
28494
35.03 %
1942
6.82 %
24732
86.80 %
1970
6.91 %
1657
5.82 %
163
0.57 %
Scotland - Scotland
West lothian
PAR-NEW-IN
1
27
0.03 %
0
0.00 %
16
59.26 %
11
40.74 %
11
40.74 %
0
0.00 %
Wigtown
PAR-NEW-IN
6
842
1.04 %
20
2.38 %
738
87.65 %
96
11.40 %
82
9.74 %
2
0.24 %
Scotland
7
869
1.07 %
20
2.30 %
754
86.77 %
107
12.31 %
93
10.70 %
2
0.23 %
Grand Total
Sum:
943
81350
4773
69862
6483
5648
1067
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new
infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor,
SER-FRAUD: serial potential fraud, SER-OTHER: serial other, PAR-CHR: chronic breakdown herds - where VL reactors are disclosed at a 3rd or subsequent SI test, or SLH case after a 2nd or subsequent SI test
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol April 2024
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
92
11158
96.1%
535
4.8%
9776
87.6%
922
8.3%
814
7.3%
33
0.3%
Serial/Extended
3
453
3.9%
79
17.4%
357
78.8%
21
4.6%
17
3.8%
0
0.0 %
Total
95
11611
100.0%
614
5.3%
10133
87.3%
943
8.1%
831
7.2%
33
0.3%
Scotland
Parallel
1
31
100.0%
1
3.2%
18
58.1%
11
35.5%
11
35.5%
1
3.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
31
100.0%
1
3.2%
18
58.1%
11
35.5%
11
35.5%
1
3.2%
Wales
Parallel
137
7233
98.7%
466
6.4%
6342
87.7%
487
6.7%
415
5.7%
10
0.1%
Serial/Extended
4
94
1.3%
11
11.7%
72
76.6%
13
13.8%
10
10.6%
1
1.1%
Total
141
7327
100.0%
477
6.5%
6414
87.5%
500
6.8%
425
5.8%
11
0.2%
GB
Parallel
230
18422
97.1%
1002
5.4%
16136
87.6%
1420
7.7%
1240
6.7%
44
0.2%
Serial/Extended
7
547
2.9%
90
16.5%
429
78.4%
34
6.2%
27
4.9%
1
0.2%
Total
237
18969
100.0%
1092
5.8%
16565
87.3%
1454
7.7%
1267
6.7%
45
0.2%
95.00
141.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2024)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
384
51101
98.3%
2696
5.3%
43646
85.4%
4355
8.5%
3859
7.6%
900
1.8%
Serial/Extended
17
886
1.7%
115
13.0%
730
82.4%
51
5.8%
39
4.4%
2
0.2%
Total
401
51987
100.0%
2811
5.4%
44376
85.4%
4406
8.5%
3898
7.5%
902
1.7%
Scotland
Parallel
7
869
100.0%
20
2.3%
754
86.8%
107
12.3%
93
10.7%
2
0.2%
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
7
869
100.0%
20
2.3%
754
86.8%
107
12.3%
93
10.7%
2
0.2%
Wales
Parallel
511
26645
93.5%
1780
6.7%
23241
87.2%
1750
6.6%
1464
5.5%
160
0.6%
Serial/Extended
24
1849
6.5%
162
8.8%
1491
80.6%
220
11.9%
193
10.4%
3
0.2%
Total
535
28494
100.0%
1942
6.8%
24732
86.8%
1970
6.9%
1657
5.8%
163
0.6%
GB
Parallel
902
78615
96.6%
4496
5.7%
67641
86.0%
6212
7.9%
5416
6.9%
1062
1.4%
Serial/Extended
41
2735
3.4%
277
10.1%
2221
81.2%
271
9.9%
232
8.5%
5
0.2%
Total
943
81350
100.0%
4773
5.9%
69862
85.9%
6483
8.0%
5648
6.9%
1067
1.3%
401.00
535.00
7.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
7
547
2.9%
90
16.5%
429
78.4%
34
6.2 %
27
4.9 %
1
0.2 %
Parallel - IRs in Exit Strategy for persistent her
9
19
0.1%
6
31.6%
12
63.2%
1
5.3 %
1
5.3 %
0
Parallel New Infection
47
4771
25.2%
152
3.2%
4264
89.4%
395
8.3 %
344
7.2 %
11
0.2 %
Parallel - Other
14
866
4.6%
40
4.6%
770
88.9%
69
8.0 %
56
6.5 %
0
Parallel Persistent Infection
29
3891
20.5%
253
6.5%
3458
88.9%
210
5.4 %
173
4.4 %
7
0.2 %
Parallel - persistent infection cull area
2
189
1.0%
8
4.2%
82
43.4%
105
55.6 %
99
52.4 %
0
Parallel - Rapid Testing Two-times IRs
23
75
0.4%
29
38.7%
40
53.3%
8
10.7 %
6
8.0 %
0
Parallel - Recurrent breakdowns
64
8321
43.9%
425
5.1%
7326
88.0%
613
7.4 %
544
6.5 %
26
0.3 %
Parallel - severe IRs - in persistent herds
42
290
1.5%
89
30.7%
184
63.4%
19
6.6 %
17
5.9 %
0
Total
237
18969
100.0%
1092
5.8%
16565
87.3%
1454
7.7 %
1267
6.7 %
45
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (April 2024)
England - HRA
Extended Flexible IFN-GAMMA test
3
453
2.4%
79
17.4%
357
78.8%
21
4.6 %
17
3.8 %
0
Parallel - Other
7
777
4.1%
22
2.8%
704
90.6%
63
8.1 %
51
6.6 %
0
Parallel Persistent Infection
4
295
1.6%
42
14.2%
222
75.3%
34
11.5 %
30
10.2 %
1
0.3 %
Parallel - persistent infection cull area
2
189
1.0%
8
4.2%
82
43.4%
105
55.6 %
99
52.4 %
0
Parallel - Recurrent breakdowns
54
7085
37.4%
346
4.9%
6236
88.0%
536
7.6 %
478
6.7 %
25
0.4 %
England - HRA
70
8799
46.4%
497
5.6%
7601
86.4%
759
8.6 %
675
7.7 %
26
0.3 %
England - Edge
Parallel New Infection
16
2397
12.6%
83
3.5%
2192
91.4%
142
5.9 %
118
4.9 %
4
0.2 %
Parallel - Other
1
55
0.3%
15
27.3%
39
70.9%
1
1.8 %
1
1.8 %
0
Parallel Persistent Infection
4
254
1.3%
16
6.3%
210
82.7%
31
12.2 %
28
11.0 %
0
England - Edge
21
2706
14.3%
114
4.2%
2441
90.2%
174
6.4 %
147
5.4 %
4
0.1 %
England - LRA
Parallel New Infection
4
106
0.6%
3
2.8%
91
85.8%
10
9.4 %
9
8.5 %
3
2.8 %
England - LRA
4
106
0.6%
3
2.8%
91
85.8%
10
9.4 %
9
8.5 %
3
2.8 %
Wales - High TB
Extended Flexible IFN-GAMMA test
3
59
0.3%
7
11.9%
50
84.7%
2
3.4 %
1
1.7 %
1
1.7 %
Parallel - IRs in Exit Strategy for persistent her
6
13
0.1%
5
38.5%
7
53.8%
1
7.7 %
1
7.7 %
0
Parallel - Other
6
34
0.2%
3
8.8%
27
79.4%
5
14.7 %
4
11.8 %
0
Parallel Persistent Infection
19
3337
17.6%
195
5.8%
3024
90.6%
142
4.3 %
112
3.4 %
6
0.2 %
Parallel - Rapid Testing Two-times IRs
17
61
0.3%
24
39.3%
31
50.8%
7
11.5 %
6
9.8 %
0
Parallel - Recurrent breakdowns
9
944
5.0%
57
6.0%
836
88.6%
57
6.0 %
50
5.3 %
1
0.1 %
Parallel - severe IRs - in persistent herds
35
250
1.3%
77
30.8%
159
63.6%
16
6.4 %
14
5.6 %
0
Wales - High TB
95
4698
24.8%
368
7.8%
4134
88.0%
230
4.9 %
188
4.0 %
8
0.2 %
Wales - Intermediate TB
Parallel - IRs in Exit Strategy for persistent her
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel New Infection
6
354
1.9%
22
6.2%
315
89.0%
20
5.6 %
17
4.8 %
0
Parallel Persistent Infection
1
4
0.0%
0
0.0 %
2
50.0%
2
50.0 %
2
50.0 %
0
Parallel - Rapid Testing Two-times IRs
6
14
0.1%
5
35.7%
9
64.3%
1
7.1 %
0
0
Parallel - Recurrent breakdowns
1
292
1.5%
22
7.5%
254
87.0%
20
6.8 %
16
5.5 %
0
Parallel - severe IRs - in persistent herds
6
39
0.2%
11
28.2%
25
64.1%
3
7.7 %
3
7.7 %
0
Wales - Intermediate TB
21
705
3.7%
60
8.5%
607
86.1%
46
6.5 %
38
5.4 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
35
0.2%
4
11.4%
22
62.9%
11
31.4 %
9
25.7 %
0
Parallel - IRs in Exit Strategy for persistent her
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
20
1883
9.9%
43
2.3%
xx-xxxx-xxxx
87.5%
212
11.3 %
189
10.0 %
3
0.2 %
Parallel Persistent Infection
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
1
100.0%
0
0.0 %
0
0
0
Wales - Low TB
25
1924
10.1%
49
2.5%
1673
87.0%
224
11.6 %
199
10.3 %
3
0.2 %
Scotland - Scotland
Parallel New Infection
1
31
0.2%
1
3.2%
18
58.1%
11
35.5 %
11
35.5 %
1
3.2 %
Scotland - Scotland
1
31
0.2%
1
3.2%
18
58.1%
11
35.5 %
11
35.5 %
1
3.2 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
36
2467
3.0%
276
11.2%
1961
79.5%
262
10.6 %
226
9.2 %
4
0.2 %
Parallel – chronic breakdown
5
411
0.5%
51
12.4%
337
82.0%
26
6.3 %
23
5.6 %
0
Parallel - Herd or Group Slaughter
2
44
0.1%
6
13.6%
34
77.3%
4
9.1 %
4
9.1 %
0
Parallel - IRs in Exit Strategy for persistent herds
37
131
0.2%
62
47.3%
61
46.6%
8
6.1 %
8
6.1 %
0
Parallel New Infection
164
16333
20.1%
567
3.5%
14168
86.7%
1737
10.6 %
1522
9.3 %
76
0.5 %
Parallel - new infection cull area
5
381
0.5%
26
6.8%
331
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
29
2703
3.3%
185
6.8%
2323
85.9%
233
8.6 %
194
7.2 %
1
0.0 %
Parallel Persistent Infection
133
16660
20.5%
1144
6.9%
14405
86.5%
1158
7.0 %
979
5.9 %
132
0.8 %
Parallel - persistent infection cull area
11
2614
3.2%
102
3.9%
1818
69.5%
217
8.3 %
198
7.6 %
496
19.0 %
Parallel - Rapid Testing Two-times IRs
84
268
0.3%
100
37.3%
152
56.7%
19
7.1 %
16
6.0 %
0
Parallel - Recurrent breakdowns
283
37888
46.6%
1819
4.8%
33337
88.0%
2701
7.1 %
2377
6.3 %
355
0.9 %
Parallel - severe IRs - in persistent herds
149
1182
1.5%
434
36.7%
675
57.1%
80
6.8 %
71
6.0 %
2
0.2 %
Serial - Other
5
268
0.3%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
Total
943
81350
100.0%
4773
5.9%
69862
85.9%
6483
8.0 %
5648
6.9 %
1067
1.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2024)
England - HRA
Extended Flexible IFN-GAMMA test
12
618
0.8%
114
18.4%
470
76.1%
42
6.8 %
33
5.3 %
1
0.2 %
Parallel – chronic breakdown
5
411
0.5%
51
12.4%
337
82.0%
26
6.3 %
23
5.6 %
0
Parallel - new infection cull area
5
381
0.5%
26
6.8%
331
86.9%
29
7.6 %
24
6.3 %
0
Parallel - Other
12
2036
2.5%
135
6.6%
1736
85.3%
193
9.5 %
165
8.1 %
0
Parallel Persistent Infection
31
3921
4.8%
375
9.6%
3212
81.9%
377
9.6 %
329
8.4 %
5
0.1 %
Parallel - persistent infection cull area
11
2614
3.2%
102
3.9%
1818
69.5%
217
8.3 %
198
7.6 %
496
19.0 %
Parallel - Recurrent breakdowns
196
26963
33.1%
1410
5.2%
23392
86.8%
2061
7.6 %
1826
6.8 %
335
1.2 %
England - Edge
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
7
77.8%
2
22.2 %
2
22.2 %
0
Parallel New Infection
63
7823
9.6%
300
3.8%
6758
86.4%
846
10.8 %
732
9.4 %
33
0.4 %
Parallel - Other
2
323
0.4%
26
8.0%
292
90.4%
6
1.9 %
5
1.5 %
0
Parallel Persistent Infection
14
1892
2.3%
119
6.3%
1570
83.0%
229
12.1 %
203
10.7 %
0
Parallel - Recurrent breakdowns
20
2780
3.4%
101
3.6%
2467
88.7%
210
7.6 %
206
7.4 %
6
0.2 %
England - LRA
Parallel New Infection
24
1948
2.4%
51
2.6%
1726
88.6%
159
8.2 %
146
7.5 %
25
1.3 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2024) Cont.
Serial - Other
5
268
0.3%
1
0.4%
260
97.0%
9
3.4 %
6
2.2 %
1
0.4 %
England
401
51987
63.9%
2811
5.4%
44376
85.4%
4406
8.5 %
3898
7.5 %
902
1.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
16
1549
1.9%
108
7.0%
1281
82.7%
180
11.6 %
157
10.1 %
3
0.2 %
Parallel - Herd or Group Slaughter
1
35
0.0%
6
17.1%
27
77.1%
2
5.7 %
2
5.7 %
0
Parallel - IRs in Exit Strategy for persistent herd
28
95
0.1%
42
44.2%
45
47.4%
8
8.4 %
8
8.4 %
0
Parallel - Other
15
344
0.4%
24
7.0%
295
85.8%
34
9.9 %
24
7.0 %
1
0.3 %
Parallel Persistent Infection
65
9455
11.6%
579
6.1%
8360
88.4%
495
5.2 %
395
4.2 %
121
1.3 %
Parallel - Rapid Testing Two-times IRs
65
208
0.3%
84
40.4%
115
55.3%
11
5.3 %
9
4.3 %
0
Parallel - Recurrent breakdowns
56
7055
8.7%
264
3.7%
6514
92.3%
342
4.8 %
264
3.7 %
13
0.2 %
Parallel - severe IRs - in persistent herds
125
1068
1.3%
396
37.1%
608
56.9%
71
6.6 %
62
5.8 %
2
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
128
0.2%
21
16.4%
100
78.1%
8
6.3 %
7
5.5 %
0
Parallel - IRs in Exit Strategy for persistent herd
7
32
0.0%
19
59.4%
13
40.6%
0
0
0
Parallel New Infection
28
2151
2.6%
87
4.0%
1894
88.1%
185
8.6 %
160
7.4 %
10
0.5 %
Parallel Persistent Infection
21
1333
1.6%
66
5.0%
1210
90.8%
56
4.2 %
51
3.8 %
6
0.5 %
Parallel - Rapid Testing Two-times IRs
15
49
0.1%
13
26.5%
32
65.3%
5
10.2 %
4
8.2 %
0
Parallel - Recurrent breakdowns
9
901
1.1%
41
4.6%
798
88.6%
69
7.7 %
62
6.9 %
0
Parallel - severe IRs - in persistent herds
17
79
0.1%
23
29.1%
49
62.0%
7
8.9 %
7
8.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
6
172
0.2%
33
19.2%
110
64.0%
32
18.6 %
29
16.9 %
0
Parallel - IRs in Exit Strategy for persistent herd
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
42
3542
4.4%
109
3.1%
3036
85.7%
440
12.4 %
391
11.0 %
6
0.2 %
Parallel Persistent Infection
2
59
0.1%
5
8.5%
53
89.8%
1
1.7 %
1
1.7 %
0
Parallel - Rapid Testing Two-times IRs
4
11
0.0%
3
27.3%
5
45.5%
3
27.3 %
3
27.3 %
0
Parallel - Recurrent breakdowns
2
189
0.2%
3
1.6%
166
87.8%
19
10.1 %
19
10.1 %
1
0.5 %
Parallel - severe IRs - in persistent herds
7
35
0.0%
15
42.9%
18
51.4%
2
5.7 %
2
5.7 %
0
Wales
535
28494
35.0%
1942
6.8%
24732
86.8%
1970
6.9 %
1657
5.8 %
163
0.6 %
Scotland - Scotland
Parallel New Infection
7
869
1.1%
20
2.3%
754
86.8%
107
12.3 %
93
10.7 %
2
0.2 %
Scotland
7
869
1.1%
20
2.3%
754
86.8%
107
12.3 %
93
10.7 %
2
0.2 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2023 May
208
13834
112
582
4
0
0
0
0
44
2
0
2
0
493
0
37
0
0
June
189
xx-xxxx-xxxx
94
524
2
0
0
0
0
33
2
0
9
0
447
0
31
0
0
July
209
18470
108
597
15
0
0
0
0
81
4
0
10
0
438
0
47
1
0
August
252
20681
140
867
7
0
0
0
0
95
6
0
19
0
675
0
65
0
0
September
221
18070
112
638
10
0
0
0
0
52
2
0
12
0
503
0
56
1
0
October
253
18656
139
825
1
7
0
0
0
0
50
2
0
9
0
701
0
53
0
2
November
250
22290
157
1277
6
24
0
0
0
0
142
8
0
18
0
982
0
97
0
0
December
183
14037
114
892
11
0
0
0
0
69
3
0
18
0
747
0
44
0
0
2024 January
234
21995
171
1438
1
27
0
0
0
0
54
3
1
5
1
1253
1
91
0
1
February
257
20510
159
1189
21
0
0
0
0
31
0
2
11
0
1013
5
105
1
0
March
215
19876
142
1054
20
0
0
0
0
70
0
5
21
1
875
1
59
0
0
April
237
18969
146
1092
302
493
0
0
0
0
2
0
0
0
0
72
5
0
0
0
Totals (last
12 months)
2708
223869
1594
10975
310
641
0
0
0
0
723
32
8
134
2
8199
12
685
3
3
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
1072
146648
723
6782
367
0
0
0
0
691
17
6
82
0
5228
5
66
1
0
Scotland
61
7628
33
148
6
0
0
0
0
7
0
0
2
0
89
0
41
1
0
Wales
1575
69593
838
4045
268
0
0
0
0
25
15
2
50
2
2882
7
578
1
3
Totals
2708
223869
1594
10975
641
0
0
0
0
723
32
8
134
2
8199
12
685
3
3
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
133
129
97
132
103
165
90
130
111
158
99
114
139
109
130
144
98
107
80
100
81
120
91
109
105
176
118
147
107
159
85
118
96
112
95
94
101
108
112
140
109
112
114
139
93
157
69
114
63
171
98
159
73
142
91
146
0
50
100
150
200
250
300
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2022
2023
2024
IFNg Positive herds
IFNg Negative herds
17250
852
xx-xxxx-xxxx
966
21357
1108
14149
687
22824
918
17067
590
14705
485
18751
758
15643
672
15663
620
15019
673
12991
545
21877
1138
16071
1036
18641
867
15246
782
13167
582
15789
524
17758
597
19561
867
17206
638
17659
825
20756
1277
13035
892
20308
1434
18508
1189
18373
1054
17402
1092
0
5000
10000
15000
20000
25000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2022
2023
2024
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
10
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
2022
2023
2024
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
Wiltshire
Warks
Staffs
Shropshire
Powys
Oxon
Northants
Leics & Rut
Heref
Gwynedd
Gloucs
Durham
Devon
Cumbria
Conwy
Ceredigion
Bucks
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during April 2024 (% submission per County)
County
%Resampled %Retested
Avon
3.55 %
4.63 %
Bucks
6.29 %
7.43 %
Carms
2.86 %
3.55 %
Ceredigion
5.57 %
6.49 %
Cheshire
11.02 %
12.20 %
Conwy
Corn & Scilly
7.94 %
8.41 %
Cumbria
Derbyshire
Devon
15.46 %
16.72 %
Dorset
5.52 %
6.48 %
Durham
7.07 %
8.08 %
E Sussex
3.68 %
5.67 %
Gloucs
6.84 %
6.84 %
Gwent
2.70 %
3.09 %
Gwynedd
9.28 %
10.56 %
Hampshire
8.33 %
8.33 %
Heref
11.40 %
12.13 %
Humberside
33.33 %
33.33 %
Leics & Rut
5.91 %
7.24 %
NE Wales
9.95 %
11.10 %
Northants
3.45 %
3.69 %
Notts
10.53 %
10.53 %
Oxon
66.67 %
66.67 %
Pembs
5.44 %
6.83 %
Powys
4.67 %
5.68 %
S Glamorgan
20.00 %
Shropshire
4.73 %
5.82 %
Somerset excl
11.91 %
12.48 %
Staffs
5.50 %
5.50 %
S Wales
Warks
1.82 %
1.82 %
Wigtown
35.48 %
35.48 %
Wiltshire
3.37 %
4.28 %

Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
11279
614
5.4%
11611
614
5.3%
Wales
7184
477
6.6%
7327
477
6.5%
Scotland
31
1
3.2%
31
1
3.2%
GB
18494
1092
5.9%
18969
1092
5.8%
Table 6a (i): Number of animals and submissions tested in April 2024
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
49273
2807
5.7%
51987
2811
5.4%
Wales
27159
1942
7.2%
28494
1942
6.8%
Scotland
808
20
2.5%
869
20
2.3%
GB
77237
4769
6.2%
81350
4773
5.9%
Table 6b (i): Number of animals and submissions tested in 2024 Up to end of April
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
8642
325
3.8%
8681
325
3.7%
Wales
5220
331
6.3%
5263
331
6.3%
Scotland
2166
126
5.8%
2250
126
5.6%
GB
16028
782
4.9%
16194
782
4.8%
Table 6a (ii): Number of animals and submissions tested in April 2023
Table 6b (ii): Number of animals and submissions tested in 2023 Up to end of April
Country
No.
animals
No of IFNg+
animals
% Positive
animals
No. samples
No of IFNg+
samples
% Positive
samples
England
47033
2070
4.4%
49597
2070
4.2%
Wales
21390
1411
6.6%
22702
1411
6.2%
Scotland
3491
342
9.8%
5023
342
6.8%
GB
71896
3823
5.3%
77322
3823
4.9%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SE
1
15
0
0
Devon
PRV-SE
1
99
8
8.08 %
Gloucestershire
PRV-SE
1
78
7
8.97 %
Total
3
192
15
7.81 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples April 2024
Table 7b Number of private samples 2024
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SE
1
15
0
0
PRV-SP
1
1
0
0
Devon
PRV-SE
2
100
8
8.00 %
East Sussex
PRV-SP
1
1
0
0
Gloucestershire
PRV-SE
1
78
7
8.97 %
North Yorkshire
PRV-SP
1
1
0
0
Oxfordshire
PRV-SE
1
5
0
0
Wiltshire
PRV-SP
1
1
0
0
Worcestershire
PRV-SE
1
1
0
0
Total
10
203
15
7.39 %

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Oxfordshire
BOV-P
1
114
10
8.8%
England
1
114
10
8.8%
Wales
Carmarthenshire
BOV-IR-C
7
49
4
8.2%
BOV-IR-EX
3
11
4
36.4%
BOV-P
1
57
0
0
Ceredigion
BOV-IR-C
2
8
3
37.5%
BOV-IR-EX
1
8
4
50.0%
North East Wales
BOV-IR-C
3
19
2
10.5%
BOV-IR-EX
1
1
0
0
BOV-P
1
58
3
5.2%
Pembrokeshire
BOV-IR-C
11
124
26
21.0%
BOV-OTHER
1
17
0
0
Powys
BOV-IR-C
2
6
2
33.3%
South Wales
BOV-IR-C
2
38
7
18.4%
Wales
35
396
55
13.9%
All GB
36
510
65
12.7 %
Table 8a Number of Cattle Antibody Tests April 2024
Table 8b Number of Cattle Antibody Tests 2024
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cumbria
BOV-OTHER
4
885
22
2.5%
Oxfordshire
BOV-P
1
114
10
8.8%
England
5
999
32
3.2%
Wales
Carmarthenshire
BOV-IR-C
20
172
31
18.0%
BOV-IR-EX
5
18
6
33.3%
BOV-OTHER
1
7
0
0
BOV-P
1
57
0
0
Ceredigion
BOV-IR-C
7
39
5
12.8%
BOV-IR-EX
5
36
11
30.6%
North East Wales
BOV-IR-C
7
49
10
20.4%
BOV-IR-EX
2
8
1
12.5%
BOV-OTHER
1
9
5
55.6%
BOV-P
3
111
7
6.3%
North West Wales
BOV-IR-C
1
5
0
0
Pembrokeshire
BOV-IR-C
64
688
132
19.2%
BOV-IR-EX
7
23
3
13.0%
BOV-OTHER
2
85
3
3.5%
BOV-P
2
42
6
14.3%
Powys
BOV-IR-C
10
66
10
15.2%
BOV-IR-EX
2
16
1
6.3%
South Wales
BOV-IR-C
5
50
8
16.0%
BOV-IR-EX
1
16
4
25.0%
Wales
146
1,497
243
16.2%
All GB
151
2,496
275
11.0 %