Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - September 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 181
Report Period 1st - 30th September 2021
Issued 10 October 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
England - parallel blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-POSTM
Post movement blood test of cattle (coupled
with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Test Criteria

Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
September
2021
%
Total 2021
%
Submissions
274
3109
Samples
Total (%)
15805
100.00 %
234727
100.00 %
England
11798
74.65 %
182959
77.95 %
Wales
3899
24.67 %
50602
21.56 %
Scotland
108
0.68 %
1166
0.50 %
Parallel Tests
PAR-CUL-N
4347
27.50 %
104242
44.41 %
PAR-CUL-P
459
2.90 %
9520
4.06 %
PAR-HERD-S
0
0.00 %
1624
0.69 %
PAR-IR-EX
45
0.28 %
318
0.14 %
PAR-IR-SEV
394
2.49 %
2195
0.94 %
PAR-NEW-IN
3269
20.68 %
66520
28.34 %
PAR-OTHER
635
4.02 %
6692
2.85 %
PAR-PERSIS
2944
18.63 %
28537
12.16 %
PAR-POSTM
43
0.27 %
139
0.06 %
PAR-RAPID
74
0.47 %
819
0.35 %
PAR-RECUR
3190
20.18 %
9263
3.95 %
Total(% of all samples)
15400
97.44 %
229869
97.93 %
Serial/Extended Tests
SER-ANOM
7
0.04 %
26
0.01 %
SER-FLEXI
398
2.52 %
4776
2.03 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
52
0.02 %
Total(% of all samples)
405
2.56 %
4858
2.07 %
Total
15805
100.00 %
234727
100.00 %
Retests
Total (% of all samples)
1014
6.42 %
xx-xxxx-xxxx
7.17 %
England
742
4.69 %
13556
5.78 %
Wales
265
1.68 %
3192
1.36 %
Scotland
7
0.04 %
86
0.04 %
Resamples
Total (% of all samples)
764
4.83 %
13770
5.87 %
England
561
3.55 %
11158
4.75 %
Wales
199
1.26 %
2539
1.08 %
Scotland
4
0.03 %
73
0.03 %
Rejects
Total (% of all samples)
21
0.13 %
721
0.31 %
England
9
0.06 %
588
0.25 %
Wales
12
0.08 %
123
0.05 %
Scotland
0
0.00 %
10
0.00 %
Table 1. Monthly Totals for September 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-ANOM
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
4347
27.5 %
PAR-CUL-P
459
2.9 %
PAR-IR-EX
45
0.3 %
PAR-IR-SEV
394
2.5 %
PAR-NEW-IN
3269
20.7 %
PAR-OTHER
635
4.0 %
PAR-PERSIS
2944
18.6 %
PAR-POSTM
43
0.3 %
PAR-RAPID
74
0.5 %
PAR-RECUR
3190
20.2 %
SER-ANOM
7
0.0 %
SER-FLEXI
398
2.5 %
Total
15805
100.00 %
Figure 1. Number of samples submitted in each category during September 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
3000
3500
4000
Ayrshire
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Heref
Hampshire
Gloucs
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during September 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
1
4
0.03 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
171
1.08 %
10
5.85 %
156
91.23 %
5
2.92 %
5
2.92 %
0
0.00 %
PAR-PERSIS
2
295
1.87 %
36
12.20 %
248
84.07 %
13
4.41 %
11
3.73 %
0
0.00 %
SER-ANOM
1
7
0.04 %
0
0.00 %
6
85.71 %
1
14.29 %
1
14.29 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
5
754
4.77 %
44
5.84 %
678
89.92 %
48
6.37 %
32
4.24 %
0
0.00 %
PAR-CUL-P
1
8
0.05 %
0
0.00 %
5
62.50 %
3
37.50 %
3
37.50 %
0
0.00 %
PAR-RECUR
3
536
3.39 %
73
13.62 %
445
83.02 %
19
3.54 %
18
3.36 %
0
0.00 %
Devon
PAR-CUL-N
14
1,694
10.72 %
32
1.89 %
1563
92.27 %
128
7.56 %
98
5.79 %
1
0.06 %
PAR-CUL-P
2
99
0.63 %
1
1.01 %
83
83.84 %
13
13.13 %
13
13.13 %
2
2.02 %
PAR-NEW-IN
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
PAR-PERSIS
4
1,044
6.61 %
60
5.75 %
930
89.08 %
74
7.09 %
54
5.17 %
0
0.00 %
PAR-RECUR
3
591
3.74 %
19
3.21 %
547
92.55 %
34
5.75 %
25
4.23 %
0
0.00 %
SER-FLEXI
3
102
0.65 %
12
11.76 %
87
85.29 %
5
4.90 %
3
2.94 %
0
0.00 %
Dorset
PAR-CUL-N
2
19
0.12 %
5
26.32 %
6
31.58 %
8
42.11 %
8
42.11 %
0
0.00 %
PAR-CUL-P
1
6
0.04 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
Gloucs
PAR-CUL-N
4
242
1.53 %
17
7.02 %
217
89.67 %
12
4.96 %
8
3.31 %
0
0.00 %
PAR-RECUR
1
193
1.22 %
2
1.04 %
180
93.26 %
13
6.74 %
9
4.66 %
2
1.04 %
Heref
PAR-RECUR
1
286
1.81 %
22
7.69 %
253
88.46 %
16
5.59 %
11
3.85 %
0
0.00 %
Shropshire
PAR-OTHER
1
4
0.03 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RECUR
4
207
1.31 %
13
6.28 %
176
85.02 %
22
10.63 %
18
8.70 %
0
0.00 %
Somerset excl N
PAR-CUL-N
8
1,244
7.87 %
86
6.91 %
1105
88.83 %
72
5.79 %
52
4.18 %
1
0.08 %
PAR-CUL-P
2
18
0.11 %
0
0.00 %
11
61.11 %
7
38.89 %
7
38.89 %
0
0.00 %
PAR-RECUR
1
341
2.16 %
11
3.23 %
320
93.84 %
19
5.57 %
10
2.93 %
0
0.00 %
SER-FLEXI
2
4
0.03 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Staffs
PAR-RECUR
1
8
0.05 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Wiltshire
PAR-CUL-N
2
157
0.99 %
14
8.92 %
138
87.90 %
8
5.10 %
5
3.18 %
0
0.00 %
Table 2a. Summary by County for September 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
4
245
1.55 %
16
6.53 %
214
87.35 %
17
6.94 %
14
5.71 %
1
0.41 %
Worcs
PAR-PERSIS
3
323
2.04 %
45
13.93 %
263
81.42 %
16
4.95 %
15
4.64 %
0
0.00 %
England - Edge
Berks
PAR-PERSIS
1
128
0.81 %
1
0.78 %
126
98.44 %
2
1.56 %
1
0.78 %
0
0.00 %
Bucks
PAR-NEW-IN
3
228
1.44 %
13
5.70 %
205
89.91 %
10
4.39 %
9
3.95 %
1
0.44 %
Cheshire
PAR-CUL-N
3
237
1.50 %
10
4.22 %
221
93.25 %
9
3.80 %
6
2.53 %
0
0.00 %
PAR-NEW-IN
2
804
5.09 %
20
2.49 %
754
93.78 %
45
5.60 %
30
3.73 %
0
0.00 %
Derbyshire
PAR-NEW-IN
2
311
1.97 %
6
1.93 %
284
91.32 %
25
8.04 %
21
6.75 %
0
0.00 %
Hampshire
PAR-NEW-IN
1
28
0.18 %
0
0.00 %
19
67.86 %
12
42.86 %
9
32.14 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
6
0.04 %
0
0.00 %
4
66.67 %
2
33.33 %
2
33.33 %
0
0.00 %
Northants
PAR-NEW-IN
1
57
0.36 %
0
0.00 %
53
92.98 %
4
7.02 %
4
7.02 %
0
0.00 %
Oxon
PAR-CUL-P
1
79
0.50 %
4
5.06 %
71
89.87 %
4
5.06 %
4
5.06 %
0
0.00 %
PAR-NEW-IN
9
678
4.29 %
35
5.16 %
620
91.45 %
38
5.60 %
23
3.39 %
0
0.00 %
Warks
PAR-NEW-IN
3
93
0.59 %
3
3.23 %
87
93.55 %
3
3.23 %
3
3.23 %
0
0.00 %
PAR-RECUR
1
64
0.40 %
1
1.56 %
59
92.19 %
5
7.81 %
4
6.25 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
2
121
0.77 %
3
2.48 %
111
91.74 %
6
4.96 %
6
4.96 %
1
0.83 %
Humberside
PAR-NEW-IN
1
118
0.75 %
4
3.39 %
109
92.37 %
6
5.08 %
5
4.24 %
0
0.00 %
Lancashire
PAR-NEW-IN
1
198
1.25 %
6
3.03 %
185
93.43 %
10
5.05 %
7
3.54 %
0
0.00 %
Lincs
PAR-NEW-IN
1
45
0.28 %
2
4.44 %
42
93.33 %
2
4.44 %
1
2.22 %
0
0.00 %
England
112
11798
74.65 %
626
5.31 %
10602
89.86 %
742
6.29 %
561
4.76 %
9
0.08 %
Wales - High TB
Carms
PAR-IR-SEV
9
85
0.54 %
13
15.29 %
67
78.82 %
5
5.88 %
5
5.88 %
0
0.00 %
PAR-OTHER
1
200
1.27 %
49
24.50 %
145
72.50 %
9
4.50 %
6
3.00 %
0
0.00 %
PAR-PERSIS
3
149
0.94 %
6
4.03 %
130
87.25 %
18
12.08 %
13
8.72 %
0
0.00 %
PAR-RAPID
7
15
0.09 %
4
26.67 %
11
73.33 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
56
0.35 %
1
1.79 %
53
94.64 %
2
3.57 %
1
1.79 %
1
1.79 %
Ceredigion
PAR-IR-EX
2
9
0.06 %
3
33.33 %
6
66.67 %
1
11.11 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
55
0.35 %
11
20.00 %
40
72.73 %
5
9.09 %
4
7.27 %
0
0.00 %
PAR-RAPID
3
4
0.03 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Gwent
PAR-PERSIS
1
5
0.03 %
0
0.00 %
1
20.00 %
4
80.00 %
4
80.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
SER-FLEXI
1
2
0.01 %
0
0.00 %
0
0.00 %
2
100.00 %
2
100.00 %
0
0.00 %
Pembs
PAR-IR-EX
5
36
0.23 %
13
36.11 %
21
58.33 %
1
2.78 %
1
2.78 %
1
2.78 %
PAR-IR-SEV
25
189
1.20 %
33
17.46 %
145
76.72 %
13
6.88 %
11
5.82 %
0
0.00 %
PAR-PERSIS
5
883
5.59 %
39
4.42 %
795
90.03 %
63
7.13 %
47
5.32 %
2
0.23 %
PAR-RAPID
21
44
0.28 %
11
25.00 %
31
70.45 %
2
4.55 %
2
4.55 %
0
0.00 %
SER-FLEXI
1
1
0.01 %
0
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
Powys
PAR-IR-SEV
3
11
0.07 %
1
9.09 %
9
81.82 %
1
9.09 %
1
9.09 %
0
0.00 %
PAR-OTHER
1
135
0.85 %
0
0.00 %
126
93.33 %
7
5.19 %
6
4.44 %
3
2.22 %
PAR-PERSIS
1
3
0.02 %
0
0.00 %
2
66.67 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
6
901
5.70 %
35
3.88 %
828
91.90 %
47
5.22 %
35
3.88 %
3
0.33 %
SER-FLEXI
3
39
0.25 %
3
7.69 %
36
92.31 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
2
125
0.79 %
5
4.00 %
116
92.80 %
9
7.20 %
4
3.20 %
0
0.00 %
PAR-RAPID
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
4
54
0.34 %
7
12.96 %
46
85.19 %
1
1.85 %
1
1.85 %
0
0.00 %
PAR-NEW-IN
3
262
1.66 %
3
1.15 %
239
91.22 %
26
9.92 %
20
7.63 %
0
0.00 %
PAR-PERSIS
2
6
0.04 %
0
0.00 %
5
83.33 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-POSTM
9
17
0.11 %
0
0.00 %
15
88.24 %
2
11.76 %
2
11.76 %
0
0.00 %
PAR-RAPID
4
8
0.05 %
2
25.00 %
4
50.00 %
3
37.50 %
2
25.00 %
0
0.00 %
PAR-RECUR
1
62
0.39 %
3
4.84 %
59
95.16 %
1
1.61 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
4
0.03 %
1
25.00 %
2
50.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Powys
PAR-POSTM
1
7
0.04 %
0
0.00 %
7
100.00 %
1
14.29 %
0
0.00 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
182
1.15 %
2
1.10 %
161
88.46 %
22
12.09 %
18
9.89 %
1
0.55 %
PAR-POSTM
4
7
0.04 %
0
0.00 %
6
85.71 %
2
28.57 %
1
14.29 %
0
0.00 %
NE Wales
PAR-NEW-IN
5
137
0.87 %
7
5.11 %
123
89.78 %
11
8.03 %
6
4.38 %
1
0.73 %
PAR-POSTM
6
12
0.08 %
0
0.00 %
12
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for September 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
2
190
1.20 %
3
1.58 %
185
97.37 %
2
1.05 %
2
1.05 %
0
0.00 %
Wales
161
3899
24.67 %
257
6.59 %
3431
88.00 %
265
6.80 %
199
5.10 %
12
0.31 %
Scotland - Scotland
Ayrshire
PAR-PERSIS
1
108
0.68 %
7
6.48 %
97
89.81 %
7
6.48 %
4
3.70 %
0
0.00 %
Scotland
1
108
0.68 %
7
6.48 %
97
89.81 %
7
6.48 %
4
3.70 %
0
0.00 %
Grand Total
Sum:
274
15805
890
14130
1014
764
21
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
5
867
0.37 %
66
7.61 %
766
88.35 %
39
4.50 %
32
3.69 %
3
0.35 %
PAR-CUL-P
6
565
0.24 %
31
5.49 %
478
84.60 %
65
11.50 %
56
9.91 %
0
0.00 %
PAR-HERD-S
3
397
0.17 %
38
9.57 %
344
86.65 %
19
4.79 %
15
3.78 %
0
0.00 %
PAR-OTHER
3
227
0.10 %
13
5.73 %
194
85.46 %
25
11.01 %
20
8.81 %
0
0.00 %
PAR-PERSIS
12
1,679
0.72 %
211
12.57 %
1335
79.51 %
148
8.81 %
124
7.39 %
9
0.54 %
SER-ANOM
3
26
0.01 %
0
0.00 %
22
84.62 %
3
11.54 %
3
11.54 %
1
3.85 %
Corn & Scilly
PAR-CUL-N
92
8,730
3.72 %
384
4.40 %
7623
87.32 %
850
9.74 %
710
8.13 %
13
0.15 %
PAR-CUL-P
19
3,572
1.52 %
282
7.89 %
3073
86.03 %
245
6.86 %
215
6.02 %
2
0.06 %
PAR-PERSIS
5
867
0.37 %
32
3.69 %
796
91.81 %
47
5.42 %
39
4.50 %
0
0.00 %
PAR-RECUR
3
536
0.23 %
73
13.62 %
445
83.02 %
19
3.54 %
18
3.36 %
0
0.00 %
SER-FLEXI
2
264
0.11 %
10
3.79 %
250
94.70 %
6
2.27 %
4
1.52 %
0
0.00 %
Devon
PAR-CUL-N
338
36,528
15.56 %
2236
6.12 %
32127
87.95 %
2465
6.75 %
2017
5.52 %
148
0.41 %
PAR-CUL-P
13
1,503
0.64 %
85
5.66 %
1294
86.09 %
153
10.18 %
121
8.05 %
3
0.20 %
PAR-NEW-IN
12
1,003
0.43 %
63
6.28 %
842
83.95 %
98
9.77 %
95
9.47 %
3
0.30 %
PAR-OTHER
5
365
0.16 %
27
7.40 %
322
88.22 %
20
5.48 %
16
4.38 %
0
0.00 %
PAR-PERSIS
12
2,418
1.03 %
107
4.43 %
2189
90.53 %
163
6.74 %
119
4.92 %
3
0.12 %
PAR-RECUR
3
591
0.25 %
19
3.21 %
547
92.55 %
34
5.75 %
25
4.23 %
0
0.00 %
SER-FLEXI
4
113
0.05 %
13
11.50 %
96
84.96 %
6
5.31 %
4
3.54 %
0
0.00 %
Dorset
PAR-CUL-N
73
8,704
3.71 %
533
6.12 %
7730
88.81 %
555
6.38 %
436
5.01 %
5
0.06 %
PAR-CUL-P
4
581
0.25 %
56
9.64 %
511
87.95 %
18
3.10 %
14
2.41 %
0
0.00 %
PAR-PERSIS
2
405
0.17 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Gloucs
PAR-CUL-N
82
8,229
3.51 %
399
4.85 %
7176
87.20 %
734
8.92 %
617
7.50 %
37
0.45 %
PAR-CUL-P
2
189
0.08 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
PAR-OTHER
1
66
0.03 %
2
3.03 %
62
93.94 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-PERSIS
7
1,485
0.63 %
92
6.20 %
1275
85.86 %
178
11.99 %
116
7.81 %
2
0.13 %
PAR-RECUR
1
193
0.08 %
2
1.04 %
180
93.26 %
13
6.74 %
9
4.66 %
2
1.04 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Heref
PAR-CUL-N
15
954
0.41 %
48
5.03 %
801
83.96 %
87
9.12 %
79
8.28 %
26
2.73 %
PAR-OTHER
3
620
0.26 %
20
3.23 %
534
86.13 %
69
11.13 %
66
10.65 %
0
0.00 %
PAR-PERSIS
2
110
0.05 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
PAR-RECUR
1
286
0.12 %
22
7.69 %
253
88.46 %
16
5.59 %
11
3.85 %
0
0.00 %
SER-OTHER
1
52
0.02 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
27
4,557
1.94 %
175
3.84 %
4144
90.94 %
253
5.55 %
220
4.83 %
18
0.39 %
PAR-OTHER
7
645
0.27 %
31
4.81 %
593
91.94 %
26
4.03 %
13
2.02 %
8
1.24 %
PAR-RECUR
6
631
0.27 %
48
7.61 %
552
87.48 %
45
7.13 %
31
4.91 %
0
0.00 %
Somerset excl N
PAR-CUL-N
98
10,122
4.31 %
696
6.88 %
9015
89.06 %
538
5.32 %
397
3.92 %
14
0.14 %
PAR-CUL-P
13
1,145
0.49 %
44
3.84 %
1033
90.22 %
79
6.90 %
64
5.59 %
4
0.35 %
PAR-NEW-IN
2
370
0.16 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.20 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
6
969
0.41 %
35
3.61 %
892
92.05 %
59
6.09 %
40
4.13 %
2
0.21 %
PAR-RECUR
1
341
0.15 %
11
3.23 %
320
93.84 %
19
5.57 %
10
2.93 %
0
0.00 %
SER-FLEXI
3
55
0.02 %
9
16.36 %
42
76.36 %
2
3.64 %
2
3.64 %
2
3.64 %
Staffs
PAR-CUL-N
75
10,756
4.58 %
386
3.59 %
9670
89.90 %
813
7.56 %
698
6.49 %
2
0.02 %
PAR-CUL-P
5
471
0.20 %
42
8.92 %
406
86.20 %
39
8.28 %
23
4.88 %
0
0.00 %
PAR-NEW-IN
3
44
0.02 %
2
4.55 %
36
81.82 %
7
15.91 %
6
13.64 %
0
0.00 %
PAR-PERSIS
1
4
0.00 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RECUR
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Wiltshire
PAR-CUL-N
34
3,364
1.43 %
238
7.07 %
2878
85.55 %
290
8.62 %
240
7.13 %
8
0.24 %
PAR-CUL-P
13
1,096
0.47 %
40
3.65 %
988
90.15 %
62
5.66 %
49
4.47 %
19
1.73 %
PAR-NEW-IN
2
191
0.08 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
11
1,751
0.75 %
76
4.34 %
1573
89.83 %
115
6.57 %
95
5.43 %
7
0.40 %
SER-FLEXI
2
172
0.07 %
13
7.56 %
148
86.05 %
1
0.58 %
1
0.58 %
10
5.81 %
Worcs
PAR-CUL-N
2
128
0.05 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
PAR-PERSIS
3
323
0.14 %
45
13.93 %
263
81.42 %
16
4.95 %
15
4.64 %
0
0.00 %
England - Edge
Berks
PAR-CUL-P
2
319
0.14 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
16
1,404
0.60 %
79
5.63 %
1228
87.46 %
107
7.62 %
97
6.91 %
0
0.00 %
PAR-PERSIS
4
247
0.11 %
9
3.64 %
234
94.74 %
7
2.83 %
4
1.62 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
12
1,588
0.68 %
43
2.71 %
1488
93.70 %
70
4.41 %
55
3.46 %
2
0.13 %
PAR-PERSIS
4
379
0.16 %
7
1.85 %
353
93.14 %
22
5.80 %
19
5.01 %
0
0.00 %
Cheshire
PAR-CUL-N
66
10,063
4.29 %
326
3.24 %
9282
92.24 %
604
6.00 %
454
4.51 %
1
0.01 %
PAR-NEW-IN
38
5,354
2.28 %
153
2.86 %
4911
91.73 %
356
6.65 %
282
5.27 %
8
0.15 %
PAR-PERSIS
4
348
0.15 %
12
3.45 %
316
90.80 %
22
6.32 %
19
5.46 %
1
0.29 %
Derbyshire
PAR-CUL-N
7
985
0.42 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
121
11,766
5.01 %
505
4.29 %
10141
86.19 %
1252
10.64 %
1052
8.94 %
68
0.58 %
E Sussex
PAR-NEW-IN
10
806
0.34 %
82
10.17 %
660
81.89 %
54
6.70 %
47
5.83 %
17
2.11 %
Hampshire
PAR-CUL-N
3
220
0.09 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
13
1,920
0.82 %
62
3.23 %
1711
89.11 %
162
8.44 %
145
7.55 %
2
0.10 %
PAR-PERSIS
1
76
0.03 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
37
4,077
1.74 %
170
4.17 %
3685
90.39 %
277
6.79 %
221
5.42 %
1
0.02 %
PAR-PERSIS
3
910
0.39 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
42
2,515
1.07 %
85
3.38 %
2283
90.78 %
207
8.23 %
146
5.81 %
1
0.04 %
PAR-PERSIS
3
184
0.08 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
7
772
0.33 %
31
4.02 %
691
89.51 %
72
9.33 %
49
6.35 %
1
0.13 %
Oxon
PAR-CUL-P
1
79
0.03 %
4
5.06 %
71
89.87 %
4
5.06 %
4
5.06 %
0
0.00 %
PAR-NEW-IN
62
4,801
2.05 %
182
3.79 %
4320
89.98 %
347
7.23 %
295
6.14 %
4
0.08 %
PAR-PERSIS
12
2,085
0.89 %
89
4.27 %
1902
91.22 %
114
5.47 %
94
4.51 %
0
0.00 %
Warks
PAR-NEW-IN
59
4,381
1.87 %
162
3.70 %
3938
89.89 %
313
7.14 %
271
6.19 %
10
0.23 %
PAR-PERSIS
3
163
0.07 %
10
6.13 %
141
86.50 %
13
7.98 %
12
7.36 %
0
0.00 %
PAR-RECUR
2
128
0.05 %
1
0.78 %
59
46.09 %
5
3.91 %
4
3.13 %
64
50.00 %
England - LRA
Beds
PAR-NEW-IN
4
579
0.25 %
22
3.80 %
497
85.84 %
78
13.47 %
56
9.67 %
4
0.69 %
Cleveland
PAR-NEW-IN
2
194
0.08 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-NEW-IN
17
2,669
1.14 %
69
2.59 %
2341
87.71 %
283
10.60 %
248
9.29 %
11
0.41 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.04 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
6
540
0.23 %
18
3.33 %
503
93.15 %
24
4.44 %
19
3.52 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.05 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Humberside
PAR-NEW-IN
1
118
0.05 %
4
3.39 %
109
92.37 %
6
5.08 %
5
4.24 %
0
0.00 %
Lancashire
PAR-NEW-IN
8
803
0.34 %
24
2.99 %
734
91.41 %
45
5.60 %
41
5.11 %
4
0.50 %
Lincs
PAR-NEW-IN
18
1,455
0.62 %
51
3.51 %
1291
88.73 %
123
8.45 %
107
7.35 %
6
0.41 %
N Yorks
PAR-NEW-IN
14
526
0.22 %
27
5.13 %
385
73.19 %
121
23.00 %
112
21.29 %
2
0.38 %
Suffolk
PAR-NEW-IN
4
328
0.14 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.09 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
1667
182959
77.95 %
9273
5.07 %
161940
88.51 %
13556
7.41 %
11158
6.10 %
588
0.32 %
Wales - High TB
Carms
PAR-IR-EX
9
48
0.02 %
21
43.75 %
24
50.00 %
2
4.17 %
2
4.17 %
1
2.08 %
PAR-IR-SEV
43
342
0.15 %
79
23.10 %
245
71.64 %
20
5.85 %
18
5.26 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
19
579
0.25 %
95
xx-xxxx-xxxx %
455
78.58 %
35
6.04 %
27
4.66 %
2
0.35 %
PAR-PERSIS
25
1,928
0.82 %
100
5.19 %
1750
90.77 %
106
5.50 %
77
3.99 %
1
0.05 %
PAR-RAPID
61
181
0.08 %
52
28.73 %
125
69.06 %
4
2.21 %
4
2.21 %
0
0.00 %
PAR-RECUR
8
581
0.25 %
27
4.65 %
530
91.22 %
33
5.68 %
22
3.79 %
2
0.34 %
SER-FLEXI
8
215
0.09 %
24
11.16 %
185
86.05 %
9
4.19 %
5
2.33 %
1
0.47 %
Ceredigion
PAR-IR-EX
6
24
0.01 %
5
20.83 %
19
79.17 %
1
4.17 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
39
287
0.12 %
73
25.44 %
198
68.99 %
20
6.97 %
14
4.88 %
2
0.70 %
PAR-OTHER
2
39
0.02 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
13
1,015
0.43 %
63
6.21 %
894
88.08 %
66
6.50 %
45
4.43 %
13
1.28 %
PAR-RAPID
33
95
0.04 %
23
24.21 %
69
72.63 %
5
5.26 %
3
3.16 %
0
0.00 %
PAR-RECUR
1
5
0.00 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
SER-FLEXI
4
147
0.06 %
13
8.84 %
129
87.76 %
5
3.40 %
4
2.72 %
1
0.68 %
Gwent
PAR-IR-EX
2
16
0.01 %
2
12.50 %
14
87.50 %
1
6.25 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
6
40
0.02 %
1
2.50 %
36
90.00 %
3
7.50 %
3
7.50 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-OTHER
4
159
0.07 %
9
5.66 %
146
91.82 %
7
4.40 %
4
2.52 %
0
0.00 %
PAR-PERSIS
11
484
0.21 %
34
7.02 %
420
86.78 %
31
6.40 %
28
5.79 %
2
0.41 %
PAR-RAPID
7
21
0.01 %
5
23.81 %
16
76.19 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
116
0.05 %
10
8.62 %
100
86.21 %
6
5.17 %
6
5.17 %
0
0.00 %
SER-FLEXI
2
28
0.01 %
5
17.86 %
19
67.86 %
4
14.29 %
4
14.29 %
0
0.00 %
Pembs
PAR-HERD-S
2
10
0.00 %
1
10.00 %
8
80.00 %
2
20.00 %
1
10.00 %
0
0.00 %
PAR-IR-EX
25
143
0.06 %
36
25.17 %
100
69.93 %
7
4.90 %
6
4.20 %
1
0.70 %
PAR-IR-SEV
141
1,157
0.49 %
269
23.25 %
846
73.12 %
51
4.41 %
41
3.54 %
1
0.09 %
PAR-OTHER
32
1,301
0.55 %
81
6.23 %
1162
89.32 %
66
5.07 %
55
4.23 %
3
0.23 %
PAR-PERSIS
98
6,111
2.60 %
253
4.14 %
5606
91.74 %
328
5.37 %
242
3.96 %
10
0.16 %
PAR-RAPID
138
378
0.16 %
115
30.42 %
250
66.14 %
20
5.29 %
13
3.44 %
0
0.00 %
PAR-RECUR
23
3,376
1.44 %
151
4.47 %
3118
92.36 %
122
3.61 %
96
2.84 %
11
0.33 %
SER-FLEXI
51
1,730
0.74 %
142
8.21 %
1533
88.61 %
74
4.28 %
48
2.77 %
7
0.40 %
Powys
PAR-HERD-S
1
10
0.00 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-IR-EX
12
78
0.03 %
21
26.92 %
53
67.95 %
6
7.69 %
4
5.13 %
0
0.00 %
PAR-IR-SEV
20
112
0.05 %
25
22.32 %
84
75.00 %
5
4.46 %
3
2.68 %
0
0.00 %
PAR-NEW-IN
1
239
0.10 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
17
939
0.40 %
41
4.37 %
840
89.46 %
65
6.92 %
49
5.22 %
9
0.96 %
PAR-PERSIS
38
2,001
0.85 %
84
4.20 %
xx-xxxx-xxxx
84.86 %
230
11.49 %
211
10.54 %
8
0.40 %
PAR-RAPID
24
46
0.02 %
10
21.74 %
34
73.91 %
3
6.52 %
2
4.35 %
0
0.00 %
PAR-RECUR
10
1,429
0.61 %
57
3.99 %
1307
91.46 %
75
5.25 %
60
4.20 %
5
0.35 %
SER-FLEXI
10
208
0.09 %
20
9.62 %
175
84.13 %
17
8.17 %
13
6.25 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.44 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
2
22
0.01 %
5
22.73 %
17
77.27 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
67
0.03 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.06 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.00 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
10
803
0.34 %
71
8.84 %
698
86.92 %
47
5.85 %
31
3.86 %
3
0.37 %
PAR-PERSIS
6
435
0.19 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
8
11
0.00 %
3
27.27 %
8
72.73 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.09 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.04 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-IR-SEV
1
18
0.01 %
2
11.11 %
16
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
106
0.05 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.03 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
2
5
0.00 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.06 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.06 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.20 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
2
5
0.00 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
30
204
0.09 %
38
18.63 %
157
76.96 %
11
5.39 %
9
4.41 %
0
0.00 %
PAR-NEW-IN
115
9,504
4.05 %
322
3.39 %
8664
91.16 %
628
6.61 %
507
5.33 %
11
0.12 %
PAR-OTHER
5
106
0.05 %
4
3.77 %
95
89.62 %
10
9.43 %
7
6.60 %
0
0.00 %
PAR-PERSIS
23
1,786
0.76 %
49
2.74 %
1654
92.61 %
113
6.33 %
83
4.65 %
0
0.00 %
PAR-POSTM
21
57
0.02 %
1
1.75 %
52
91.23 %
4
7.02 %
4
7.02 %
0
0.00 %
PAR-RAPID
17
60
0.03 %
13
21.67 %
44
73.33 %
4
6.67 %
3
5.00 %
0
0.00 %
PAR-RECUR
5
267
0.11 %
24
8.99 %
232
86.89 %
13
4.87 %
11
4.12 %
0
0.00 %
SER-FLEXI
20
749
0.32 %
47
6.28 %
681
90.92 %
34
4.54 %
21
2.80 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
20
1,884
0.80 %
53
2.81 %
xx-xxxx-xxxx
89.28 %
163
8.65 %
146
7.75 %
3
0.16 %
PAR-PERSIS
1
14
0.01 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-POSTM
7
18
0.01 %
2
11.11 %
15
83.33 %
2
11.11 %
1
5.56 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
5
196
0.08 %
8
4.08 %
169
86.22 %
23
11.73 %
18
9.18 %
1
0.51 %
S Glamorgan
PAR-RAPID
3
9
0.00 %
5
55.56 %
4
44.44 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
221
0.09 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-IR-SEV
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
126
0.05 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
57
0.02 %
1
1.75 %
55
96.49 %
1
1.75 %
1
1.75 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
37
1,912
0.81 %
54
2.82 %
1727
90.32 %
159
8.32 %
127
6.64 %
4
0.21 %
PAR-OTHER
2
51
0.02 %
0
0.00 %
50
98.04 %
2
3.92 %
1
1.96 %
0
0.00 %
PAR-POSTM
6
14
0.01 %
0
0.00 %
13
92.86 %
2
14.29 %
1
7.14 %
0
0.00 %
PAR-RAPID
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
65
0.03 %
7
10.77 %
53
81.54 %
6
9.23 %
5
7.69 %
0
0.00 %
NE Wales
PAR-HERD-S
4
111
0.05 %
9
8.11 %
99
89.19 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-NEW-IN
38
2,717
1.16 %
134
4.93 %
2377
87.49 %
241
8.87 %
201
7.40 %
5
0.18 %
PAR-OTHER
4
152
0.06 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.02 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
PAR-POSTM
20
50
0.02 %
2
4.00 %
47
94.00 %
4
8.00 %
1
2.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
7
797
0.34 %
52
6.52 %
706
88.58 %
45
5.65 %
39
4.89 %
0
0.00 %
Wales
1428
50602
21.56 %
3076
6.08 %
44864
88.66 %
3192
6.31 %
2539
5.02 %
123
0.24 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.08 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
7
846
0.36 %
113
13.36 %
666
78.72 %
65
7.68 %
58
6.86 %
9
1.06 %
PAR-PERSIS
1
108
0.05 %
7
6.48 %
97
89.81 %
7
6.48 %
4
3.70 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.01 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
14
1166
0.50 %
136
11.66 %
947
81.22 %
86
7.38 %
73
6.26 %
10
0.86 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Grand Total
Sum:
xx-xxxx-xxxx
12485
207751
xx-xxxx-xxxx
13770
721
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol September 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
106
11685
99.0%
614
5.3%
10505
89.9%
736
6.3%
557
4.8%
9
0.1%
Serial/Extended
6
113
1.0%
12
10.6%
97
85.8%
6
5.3%
4
3.5%
0
0.0 %
Total
112
11798
100.0%
626
5.3%
10602
89.9%
742
6.3%
561
4.8%
9
0.1%
Scotland
Parallel
1
108
100.0%
7
6.5%
97
89.8%
7
6.5%
4
3.7%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
1
108
100.0%
7
6.5%
97
89.8%
7
6.5%
4
3.7%
0
0.0 %
Wales
Parallel
150
3607
92.5%
249
6.9%
3155
87.5%
257
7.1%
192
5.3%
11
0.3%
Serial/Extended
11
292
7.5%
8
2.7%
276
94.5%
8
2.7%
7
2.4%
1
0.3%
Total
161
3899
100.0%
257
6.6%
3431
88.0%
265
6.8%
199
5.1%
12
0.3%
GB
Parallel
257
15400
97.4%
870
5.6%
13757
89.3%
1000
6.5%
753
4.9%
20
0.1%
Serial/Extended
17
405
2.6%
20
4.9%
373
92.1%
14
3.5%
11
2.7%
1
0.2%
Total
274
15805
100.0%
890
5.6%
14130
89.4%
1014
6.4%
764
4.8%
21
0.1%
112.00
161.00
1.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.6%
9228
5.1% 161331
88.5%
13537
7.4%
11143
6.1%
575
0.3%
Serial/Extended
15
682
0.4%
45
6.6%
609
89.3%
19
2.8%
15
2.2%
13
1.9%
Total
1667 182959
100.0%
9273
5.1% 161940
88.5%
13556
7.4%
11158
6.1%
588
0.3%
Scotland
Parallel
13
1162
99.7%
136
11.7%
944
81.2%
86
7.4%
73
6.3%
9
0.8%
Serial/Extended
1
4
0.3%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
14
1166
100.0%
136
11.7%
947
81.2%
86
7.4%
73
6.3%
10
0.9%
Wales
Parallel
1315
46430
91.8%
2738
5.9%
41184
88.7%
2989
6.4%
2395
5.2%
113
0.2%
Serial/Extended
113
4172
8.2%
338
8.1%
3680
88.2%
203
4.9%
144
3.5%
10
0.2%
Total
1428
50602
100.0%
3076
6.1%
44864
88.7%
3192
6.3%
2539
5.0%
123
0.2%
GB
Parallel
xx-xxxx-xxxx
97.9% 12102
5.3% 203459
88.5%
16612
7.2%
13611
5.9%
697
0.3%
Serial/Extended
129
4858
2.1%
383
7.9%
4292
88.3%
222
4.6%
159
3.3%
24
0.5%
Total
xx-xxxx-xxxx
100.0% 12485
5.3% 207751
88.5%
xx-xxxx-xxxx
7.2%
13770
5.9%
721
0.3%
#######
#######
14.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Extended Flexible IFN-GAMMA test
16
398
2.5%
20
5.0%
367
92.2%
13
3.3 %
10
2.5 %
1
0.3 %
Parallel - IRs in Exit Strategy for persistent her
7
45
0.3%
16
35.6%
27
60.0%
2
4.4 %
1
2.2 %
1
2.2 %
Parallel New Infection
43
3269
20.7%
104
3.2%
2996
91.6%
223
6.8 %
165
5.0 %
4
0.1 %
Parallel - new infection cull area
38
4347
27.5%
208
4.8%
3928
90.4%
285
6.6 %
209
4.8 %
2
0.0 %
Parallel - Other
6
635
4.0%
64
10.1%
545
85.8%
32
5.0 %
23
3.6 %
3
0.5 %
Parallel Persistent Infection
23
2944
18.6%
194
6.6%
2597
88.2%
199
6.8 %
151
5.1 %
2
0.1 %
Parallel - persistent infection cull area
12
459
2.9%
21
4.6%
392
85.4%
46
10.0 %
43
9.4 %
3
0.7 %
Parallel - Rapid Testing Two-times IRs
38
74
0.5%
19
25.7%
51
68.9%
5
6.8 %
4
5.4 %
0
Parallel - Recurrent breakdowns
23
3190
20.2%
179
5.6%
2874
90.1%
178
5.6 %
132
4.1 %
5
0.2 %
Parallel - severe IRs - in persistent herds
47
394
2.5%
65
16.5%
307
77.9%
25
6.3 %
22
5.6 %
0
PAR-POSTM
20
43
0.3%
0
0.0 %
40
93.0%
5
11.6 %
3
7.0 %
0
Total
274
15805
100.0%
890
5.6%
14130
89.4%
1014
6.4 %
764
4.8 %
21
0.1 %
Table 4a. Summaryof IFN Gamma results by submission reason (September 2021)
England - HRA
Anomalous reaction
1
7
0.0%
0
0.0 %
6
85.7%
1
14.3 %
1
14.3 %
0
Extended Flexible IFN-GAMMA test
5
106
0.7%
12
11.3%
91
85.8%
5
4.7 %
3
2.8 %
0
Parallel New Infection
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel - new infection cull area
35
4110
26.0%
198
4.8%
3707
90.2%
276
6.7 %
203
4.9 %
2
0.0 %
Parallel - Other
2
175
1.1%
10
5.7%
158
90.3%
7
4.0 %
7
4.0 %
0
Parallel Persistent Infection
9
1662
10.5%
141
8.5%
1441
86.7%
103
6.2 %
80
4.8 %
0
Parallel - persistent infection cull area
11
380
2.4%
17
4.5%
321
84.5%
42
11.1 %
39
10.3 %
3
0.8 %
Parallel - Recurrent breakdowns
14
2162
13.7%
140
6.5%
1928
89.2%
124
5.7 %
92
4.3 %
2
0.1 %
England - HRA
78
8603
54.4%
518
6.0%
7652
88.9%
559
6.5 %
426
5.0 %
7
0.1 %
England - Edge
Parallel New Infection
23
2205
14.0%
77
3.5%
2026
91.9%
139
6.3 %
101
4.6 %
1
0.0 %
Parallel - new infection cull area
3
237
1.5%
10
4.2%
221
93.2%
9
3.8 %
6
2.5 %
0
Parallel Persistent Infection
1
128
0.8%
1
0.8%
126
98.4%
2
1.6 %
1
0.8 %
0
Parallel - persistent infection cull area
1
79
0.5%
4
5.1%
71
89.9%
4
5.1 %
4
5.1 %
0
Parallel - Recurrent breakdowns
1
64
0.4%
1
1.6%
59
92.2%
5
7.8 %
4
6.3 %
0
England - Edge
29
2713
17.2%
93
3.4%
2503
92.3%
159
5.9 %
116
4.3 %
1
0.0 %
England - LRA
Parallel New Infection
5
482
3.0%
15
3.1%
447
92.7%
24
5.0 %
19
3.9 %
1
0.2 %
England - LRA
5
482
3.0%
15
3.1%
447
92.7%
24
5.0 %
19
3.9 %
1
0.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
7
98
0.6%
4
4.1%
89
90.8%
5
5.1 %
4
4.1 %
1
1.0 %
Parallel - IRs in Exit Strategy for persistent her
7
45
0.3%
16
35.6%
27
60.0%
2
4.4 %
1
2.2 %
1
2.2 %
Parallel - Other
2
335
2.1%
49
14.6%
271
80.9%
16
4.8 %
12
3.6 %
3
0.9 %
Parallel Persistent Infection
10
1040
6.6%
45
4.3%
928
89.2%
86
8.3 %
65
6.3 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
33
65
0.4%
17
26.2%
46
70.8%
2
3.1 %
2
3.1 %
0
Parallel - Recurrent breakdowns
7
902
5.7%
35
3.9%
828
91.8%
48
5.3 %
36
4.0 %
3
0.3 %
Parallel - severe IRs - in persistent herds
43
340
2.2%
58
17.1%
261
76.8%
24
7.1 %
21
6.2 %
0
Wales - High TB
109
2825
17.9%
224
7.9%
2450
86.7%
183
6.5 %
141
5.0 %
10
0.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
2
50.0%
1
25.0 %
1
25.0 %
0
Parallel New Infection
3
262
1.7%
3
1.1%
239
91.2%
26
9.9 %
20
7.6 %
0
Parallel - Other
2
125
0.8%
5
4.0%
116
92.8%
9
7.2 %
4
3.2 %
0
Parallel Persistent Infection
2
6
0.0%
0
0.0 %
5
83.3%
1
16.7 %
1
16.7 %
0
Parallel - Rapid Testing Two-times IRs
5
9
0.1%
2
22.2%
5
55.6%
3
33.3 %
2
22.2 %
0
Parallel - Recurrent breakdowns
1
62
0.4%
3
4.8%
59
95.2%
1
1.6 %
0
0
Parallel - severe IRs - in persistent herds
4
54
0.3%
7
13.0%
46
85.2%
1
1.9 %
1
1.9 %
0
PAR-POSTM
10
24
0.2%
0
0.0 %
22
91.7%
3
12.5 %
2
8.3 %
0
Wales - Intermediate TB
29
546
3.5%
21
3.8%
494
90.5%
45
8.2 %
31
5.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
190
1.2%
3
1.6%
185
97.4%
2
1.1 %
2
1.1 %
0
Parallel New Infection
11
319
2.0%
9
2.8%
284
89.0%
33
10.3 %
24
7.5 %
2
0.6 %
PAR-POSTM
10
19
0.1%
0
0.0 %
18
94.7%
2
10.5 %
1
5.3 %
0
Wales - Low TB
23
528
3.3%
12
2.3%
487
92.2%
37
7.0 %
27
5.1 %
2
0.4 %
Scotland - Scotland
Parallel Persistent Infection
1
108
0.7%
7
6.5%
97
89.8%
7
6.5 %
4
3.7 %
0
Scotland - Scotland
1
108
0.7%
7
6.5%
97
89.8%
7
6.5 %
4
3.7 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
124
4776
2.0%
383
8.0%
4216
88.3%
218
4.6 %
155
3.2 %
22
0.5 %
Parallel - Herd or Group Slaughter
20
1624
0.7%
97
6.0%
1461
90.0%
74
4.6 %
61
3.8 %
5
0.3 %
Parallel - IRs in Exit Strategy for persistent herds
57
318
0.1%
89
28.0%
215
67.6%
17
5.3 %
12
3.8 %
2
0.6 %
Parallel New Infection
748
66520
28.3%
2658
4.0%
59081
88.8%
5480
8.2 %
4592
6.9 %
189
0.3 %
Parallel - new infection cull area
919 104242
44.4%
5568
5.3%
92362
88.6%
7367
7.1 %
6033
5.8 %
279
0.3 %
Parallel - Other
121
6692
2.9%
429
6.4%
5865
87.6%
440
6.6 %
371
5.5 %
27
0.4 %
Parallel Persistent Infection
317
28537
12.2%
1407
4.9%
25555
89.6%
1935
6.8 %
1515
5.3 %
60
0.2 %
Parallel - persistent infection cull area
78
9520
4.1%
622
6.5%
8294
87.1%
683
7.2 %
558
5.9 %
46
0.5 %
Parallel - Rapid Testing Two-times IRs
301
819
0.3%
231
28.2%
563
68.7%
37
4.5 %
25
3.1 %
0
Parallel - Recurrent breakdowns
80
9263
3.9%
503
5.4%
8325
89.9%
457
4.9 %
349
3.8 %
86
0.9 %
Parallel - severe IRs - in persistent herds
285
2195
0.9%
493
22.5%
1611
73.4%
110
5.0 %
88
4.0 %
3
0.1 %
PAR-POSTM
54
139
0.1%
5
3.6%
127
91.4%
12
8.6 %
7
5.0 %
0
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
xx-xxxx-xxxx
100.0%
12485
5.3%
207751
88.5%
xx-xxxx-xxxx
7.2 %
13770
5.9 %
721
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
11
604
0.3%
45
7.5%
536
88.7%
15
2.5 %
11
1.8 %
12
2.0 %
Parallel - Herd or Group Slaughter
3
397
0.2%
38
9.6%
344
86.6%
19
4.8 %
15
3.8 %
0
Parallel New Infection
19
1608
0.7%
131
8.1%
1347
83.8%
133
8.3 %
127
7.9 %
3
0.2 %
Parallel - new infection cull area
841
92939
39.6%
5173
5.6%
82042
88.3%
6627
7.1 %
5448
5.9 %
276
0.3 %
Parallel - Other
21
2390
1.0%
115
4.8%
2094
87.6%
177
7.4 %
173
7.2 %
8
0.3 %
Parallel Persistent Infection
61
10011
4.3%
613
6.1%
8794
87.8%
762
7.6 %
581
5.8 %
23
0.2 %
Parallel - persistent infection cull area
75
9122
3.9%
615
6.7%
7931
86.9%
669
7.3 %
548
6.0 %
28
0.3 %
Parallel - Recurrent breakdowns
16
2586
1.1%
175
6.8%
2304
89.1%
147
5.7 %
105
4.1 %
2
0.1 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
417
39384
16.8%
1554
3.9%
35056
89.0%
3217
8.2 %
2660
6.8 %
114
0.3 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - new infection cull area
77
11285
4.8%
395
3.5%
10314
91.4%
730
6.5 %
575
5.1 %
1
0.0 %
Parallel Persistent Infection
34
4392
1.9%
158
3.6%
4019
91.5%
253
5.8 %
213
4.8 %
2
0.0 %
Parallel - persistent infection cull area
3
398
0.2%
7
1.8%
363
91.2%
14
3.5 %
10
2.5 %
18
4.5 %
Parallel - Recurrent breakdowns
2
128
0.1%
1
0.8%
59
46.1%
5
3.9 %
4
3.1 %
64
50.0 %
England - LRA
Parallel New Infection
82
7619
3.2%
253
3.3%
6658
87.4%
774
10.2 %
674
8.8 %
34
0.4 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
1667 182959
77.9%
9273
5.1%
161940
88.5%
13556
7.4 %
11158
6.1 %
588
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
75
2328
1.0%
204
8.8%
2041
87.7%
109
4.7 %
74
3.2 %
9
0.4 %
Parallel - Herd or Group Slaughter
8
1050
0.4%
48
4.6%
956
91.0%
48
4.6 %
41
3.9 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
54
309
0.1%
85
27.5%
210
68.0%
17
5.5 %
12
3.9 %
2
0.6 %
Parallel New Infection
2
245
0.1%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
76
3084
1.3%
229
7.4%
2695
87.4%
186
6.0 %
146
4.7 %
14
0.5 %
Parallel Persistent Infection
187
11675
5.0%
536
4.6%
10497
89.9%
766
6.6 %
608
5.2 %
34
0.3 %
Parallel - Rapid Testing Two-times IRs
266
729
0.3%
206
28.3%
501
68.7%
33
4.5 %
22
3.0 %
0
Parallel - Recurrent breakdowns
45
5507
2.3%
246
4.5%
5058
91.8%
237
4.3 %
185
3.4 %
18
0.3 %
Parallel - severe IRs - in persistent herds
251
1960
0.8%
452
23.1%
1426
72.8%
99
5.1 %
79
4.0 %
3
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
27
982
0.4%
75
7.6%
880
89.6%
43
4.4 %
26
2.6 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
3
9
0.0%
4
44.4%
5
55.6%
0
0
0
Parallel New Infection
141
11981
5.1%
397
3.3%
10851
90.6%
857
7.2 %
714
6.0 %
19
0.2 %
Parallel - Other
18
1015
0.4%
81
8.0%
886
87.3%
67
6.6 %
44
4.3 %
4
0.4 %
Parallel Persistent Infection
32
2308
1.0%
91
3.9%
2108
91.3%
147
6.4 %
109
4.7 %
0
Parallel - Rapid Testing Two-times IRs
31
86
0.0%
24
27.9%
59
68.6%
4
4.7 %
3
3.5 %
0
Parallel - Recurrent breakdowns
17
1042
0.4%
81
7.8%
904
86.8%
68
6.5 %
55
5.3 %
2
0.2 %
Parallel - severe IRs - in persistent herds
33
234
0.1%
41
17.5%
184
78.6%
11
4.7 %
9
3.8 %
0
PAR-POSTM
28
75
0.0%
3
4.0%
67
89.3%
6
8.0 %
5
6.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
11
862
0.4%
59
6.8%
759
88.1%
51
5.9 %
44
5.1 %
0
Parallel - Herd or Group Slaughter
7
168
0.1%
10
6.0%
154
91.7%
6
3.6 %
4
2.4 %
0
Parallel New Infection
75
4629
2.0%
188
4.1%
4104
88.7%
400
8.6 %
328
7.1 %
9
0.2 %
Parallel - Other
6
203
0.1%
4
2.0%
190
93.6%
10
4.9 %
8
3.9 %
1
0.5 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %
Parallel - Rapid Testing Two-times IRs
4
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
PAR-POSTM
26
64
0.0%
2
3.1%
60
93.8%
6
9.4 %
2
3.1 %
0
Wales
1428
50602
21.6%
3076
6.1%
44864
88.7%
3192
6.3 %
2539
5.0 %
123
0.2 %
Scotland - Scotland
Parallel New Infection
12
1054
0.4%
129
12.2%
847
80.4%
79
7.5 %
69
6.5 %
9
0.9 %
Parallel Persistent Infection
1
108
0.0%
7
6.5%
97
89.8%
7
6.5 %
4
3.7 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
14
1166
0.5%
136
11.7%
947
81.2%
86
7.4 %
73
6.3 %
10
0.9 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
3
0
7
1
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
0
84
5
12
March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
3
0
9
1
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
0
0
4
4
1083
5
92
0
1
July
298
24841
164
1077
33
0
0
0
0
61
0
0
4
4
933
8
24
0
10
August
312
17920
167
912
3
41
0
0
0
0
35
0
6
2
1
741
81
0
0
2
September
254
15326
121
853
296
180
0
0
0
0
21
0
5
0
0
184
12
0
0
0
Totals (last
12 months)
4076
311646
2384
16233
306
563
0
0
0
0
1066
29
11
84
11
13027
107
831
10
26
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2146
240868
1409
12038
498
0
0
0
0
917
14
5
51
4
10085
10
75
0
0
Scotland
28
2915
19
190
7
0
0
0
0
46
2
0
6
0
50
5
58
3
12
Wales
1902
67863
956
4005
58
0
0
0
0
103
13
6
27
7
2892
92
698
7
14
Totals
4076
311646
2384
16233
563
0
0
0
0
1066
29
11
84
11
13027
107
831
10
26
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
160
Worcs
Warks
Somerset excl N
Powys
Oxon
NE Wales
Leics & Rut
Humberside
Hampshire
Gwent
Dorset
Derbyshire
Cheshire
Carms
Berks
Ayrshire
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during September 2021 (% submission per
County)
County
%Resampled %Retested
Avon
3.56 %
3.98 %
Ayrshire
3.70 %
6.48 %
Beds
4.96 %
4.96 %
Berks
0.78 %
1.56 %
Bucks
3.95 %
4.39 %
Carms
4.60 %
6.81 %
Ceredigion
5.88 %
8.82 %
Cheshire
3.46 %
5.19 %
Corn & Scilly
4.08 %
5.39 %
Derbyshire
6.75 %
8.04 %
Devon
5.49 %
7.22 %
Dorset
40.00 %
40.00 %
Gloucs
3.91 %
5.75 %
Gwent
77.78 %
77.78 %
Gwynedd
10.05 %
12.70 %
Hampshire
32.14 %
42.86 %
Heref
3.85 %
5.59 %
Humberside
4.24 %
5.08 %
Lancashire
3.54 %
5.05 %
Leics & Rut
33.33 %
33.33 %
Lincs
2.22 %
4.44 %
NE Wales
4.65 %
6.38 %
Northants
7.02 %
7.02 %
Oxon
3.57 %
5.55 %
Pembs
5.38 %
6.94 %
Powys
3.92 %
5.20 %
Shropshire
9.48 %
11.37 %
Somerset excl
4.29 %
6.10 %
Staffs
12.50 %
12.50 %
Warks
4.46 %
5.10 %
Wiltshire
4.73 %
6.22 %
Worcs
4.64 %
4.95 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
11709
626
5.3%
11798
626
5.3%
Wales
3806
256
6.7%
3899
257
6.6%
Scotland
108
7
6.5%
108
7
6.5%
GB
15623
889
5.7%
15805
890
5.6%
Table 6a (i): Number of animals and submissions tested in September 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
172936
9270
5.4%
182959
9273
5.1%
Wales
47226
3075
6.5%
50602
3076
6.1%
Scotland
579
136
23.5%
1166
136
11.7%
GB
220731
12481
5.7%
234727
12485
5.3%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of September
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19656
473
2.4%
19883
473
2.4%
Wales
5429
289
5.3%
5483
289
5.3%
Scotland
28
0
0.0 %
28
0.0 %
GB
25113
762
3.0%
25394
762
3.0%
Table 6a (ii): Number of animals and submissions tested in September 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of September
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
155173
6074
3.9%
163478
6074
3.7%
Wales
36230
2167
6.0%
39041
2168
5.6%
Scotland
3635
107
2.9%
4530
107
2.4%
GB
195037
8348
4.3%
207049
8349
4.0%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SE
2
70
5
7.14 %
Total
2
70
5
7.14 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples September 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
1
1
0
0
Derbyshire
PRV-SP
2
2
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
2
3
0
0
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
6
326
11
3.37 %
PRV-SP
3
5
0
0
Staffordshire
PRV-SP
2
2
0
0
Wiltshire
PRV-SE
1
10
0
0
PRV-SP
1
3
0
0
Worcestershire
PRV-SE
1
1
0
0
PRV-SP
2
3
0
0
Total
29
436
15
3.44 %
Isle of Man
SER-IOM
7
8
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Devon
BOV-P
1
40
0
0
England
1
40
0
0
Wales
Carmarthenshire
BOV-IR-C
6
92
17
18.5%
BOV-OTHER
1
54
2
3.7%
Ceredigion
BOV-IR-C
1
1
0
0
BOV-IR-EX
1
6
1
16.7%
North East Wales
BOV-IR-C
3
43
9
20.9%
BOV-P
2
69
1
1.4%
BOV-POSTM
8
14
3
21.4%
North West Wales
BOV-POSTM
2
3
2
66.7%
Pembrokeshire
BOV-IR-C
16
205
33
16.1%
BOV-IR-EX
4
51
15
29.4%
Powys
BOV-IR-C
3
32
6
18.8%
BOV-OTHER
2
87
12
13.8%
South Wales
BOV-IR-C
1
3
0
0
BOV-P
1
20
1
5.0%
Wales
51
680
102
15.0%
All GB
52
720
102
14.2 %
Table 8a Number of Cattle Antibody Tests September 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
2
412
17
4.1%
Devon
1
95
6
6.3%
BOV-P
1
40
0
0
Staffordshire
BOV-P
1
30
1
3.3%
Wiltshire
BOV-P
1
189
8
4.2%
England
6
766
32
4.2%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
34
381
87
22.8%
BOV-IR-EX
6
45
13
28.9%
BOV-OTHER
3
80
4
5.0%
BOV-P
2
87
3
3.4%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
30
276
47
17.0%
BOV-IR-EX
2
14
3
21.4%
BOV-OTHER
6
240
16
6.7%
BOV-P
1
16
0
0
North East Wales
2
24
6
25.0%
BOV-IR-C
25
238
38
16.0%
BOV-IR-EX
2
5
1
20.0%
BOV-OTHER
4
149
4
2.7%
BOV-P
13
1,186
44
3.7%
BOV-POSTM
32
82
22
26.8%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
OTHER
1
48
4
8.3%
North West Wales
BOV-P
1
42
0
0
BOV-POSTM
2
3
2
66.7%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
118
1,270
233
18.3%
BOV-IR-EX
16
123
27
22.0%
BOV-OTHER
18
855
48
5.6%
BOV-P
14
779
31
4.0%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
21
153
29
19.0%
BOV-IR-EX
3
45
9
20.0%
BOV-OTHER
5
227
30
13.2%
BOV-P
3
54
3
5.6%
BOV-POSTM
3
5
1
20.0%
South Wales
4
14
5
35.7%
BOV-IR-C
8
65
5
7.7%
BOV-IR-EX
1
21
1
4.8%
BOV-P
2
87
1
1.1%
Wales
412
6,954
761
10.9%
All GB
418
7,720
793
10.3 %