Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - October 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 182
Report Period 1st - 31st October 2021
Issued 10 November 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
England - parallel blood testing of skin
test-negative cattle in breakdown herds (in HRA
& 6-monthly-testing parts of Edge Area)
occurring within 18 months of lifting restrictions
after a previous breakdown
Wales - parallel blood testing of skin
test-negative cattle in breakdown herds
occurring within 6 months of lifting restrictions
after a previous breakdown
England - YES
Wales - NO (APHA
discretion)
PAR-POSTM
Post movement blood test of cattle (coupled
with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Test Criteria

Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
October
2021
%
Total 2021
%
Submissions
276
3385
Samples
Total (%)
19790
100.00 %
254517
100.00 %
England
13688
69.17 %
196647
77.26 %
Wales
6102
30.83 %
56704
22.28 %
Scotland
1166
0.46 %
Parallel Tests
PAR-CUL-N
2464
12.45 %
106706
41.92 %
PAR-CUL-P
5
0.03 %
9525
3.74 %
PAR-HERD-S
7
0.04 %
1631
0.64 %
PAR-IR-EX
26
0.13 %
344
0.14 %
PAR-IR-SEV
191
0.97 %
2386
0.94 %
PAR-NEW-IN
5955
30.09 %
72475
28.48 %
PAR-OTHER
1003
5.07 %
7695
3.02 %
PAR-PERSIS
3982
20.12 %
32519
12.78 %
PAR-POSTM
96
0.49 %
235
0.09 %
PAR-RAPID
110
0.56 %
929
0.37 %
PAR-RECUR
5331
26.94 %
14594
5.73 %
Total(% of all samples)
19170
96.87 %
249039
97.85 %
Serial/Extended Tests
SER-ANOM
0
0.00 %
26
0.01 %
SER-FLEXI
613
3.10 %
5389
2.12 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
7
0.04 %
59
0.02 %
Total(% of all samples)
620
3.13 %
5478
2.15 %
Total
19790
100.00 %
254517
100.00 %
Retests
Total (% of all samples)
1343
6.79 %
18177
7.14 %
England
947
4.79 %
14503
5.70 %
Wales
396
2.00 %
3588
1.41 %
Scotland
86
0.03 %
Resamples
Total (% of all samples)
1049
5.30 %
14819
5.82 %
England
753
3.80 %
11911
4.68 %
Wales
296
1.50 %
2835
1.11 %
Scotland
73
0.03 %
Rejects
Total (% of all samples)
36
0.18 %
757
0.30 %
England
27
0.14 %
615
0.24 %
Wales
9
0.05 %
132
0.05 %
Scotland
0
0.00 %
10
0.00 %
Table 1. Monthly Totals for October 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-FLEXI
SER-OTHER
Breakdown of
Reason for
submission
PAR-CUL-N
2464
12.5 %
PAR-CUL-P
5
0.0 %
PAR-HERD-S
7
0.0 %
PAR-IR-EX
26
0.1 %
PAR-IR-SEV
191
1.0 %
PAR-NEW-IN
5955
30.1 %
PAR-OTHER
1003
5.1 %
PAR-PERSIS
3982
20.1 %
PAR-POSTM
96
0.5 %
PAR-RAPID
110
0.6 %
PAR-RECUR
5331
26.9 %
SER-FLEXI
613
3.1 %
SER-OTHER
7
0.0 %
Total
19790
100.00 %
Figure 1. Number of samples submitted in each category during October 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
500
1000
1500
2000
2500
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
Oxon
Northants
Leics & Rut
Heref
Hampshire
Gr Manchester
Gloucs
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Berks
Beds
Avon
Wales
England
SER-OTHER
SER-FLEXI
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during October 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
40000
45000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Humberside
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
Essex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Beds
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
1
11
0.06 %
0
0.00 %
5
45.45 %
6
54.55 %
6
54.55 %
0
0.00 %
PAR-OTHER
2
90
0.45 %
19
21.11 %
62
68.89 %
16
17.78 %
8
8.89 %
1
1.11 %
PAR-RECUR
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
5
139
0.70 %
10
7.19 %
109
78.42 %
22
15.83 %
20
14.39 %
0
0.00 %
PAR-OTHER
1
94
0.47 %
4
4.26 %
83
88.30 %
12
12.77 %
7
7.45 %
0
0.00 %
PAR-PERSIS
5
340
1.72 %
9
2.65 %
316
92.94 %
21
6.18 %
15
4.41 %
0
0.00 %
PAR-RECUR
1
14
0.07 %
0
0.00 %
7
50.00 %
7
50.00 %
7
50.00 %
0
0.00 %
SER-FLEXI
1
329
1.66 %
18
5.47 %
300
91.19 %
14
4.26 %
11
3.34 %
0
0.00 %
SER-OTHER
1
7
0.04 %
0
0.00 %
6
85.71 %
3
42.86 %
1
14.29 %
0
0.00 %
Devon
PAR-CUL-N
4
289
1.46 %
12
4.15 %
254
87.89 %
26
9.00 %
23
7.96 %
0
0.00 %
PAR-CUL-P
1
5
0.03 %
1
20.00 %
4
80.00 %
2
40.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
4
386
1.95 %
6
1.55 %
356
92.23 %
27
6.99 %
24
6.22 %
0
0.00 %
PAR-RECUR
7
373
1.88 %
8
2.14 %
337
90.35 %
39
10.46 %
27
7.24 %
1
0.27 %
SER-FLEXI
1
4
0.02 %
0
0.00 %
3
75.00 %
1
25.00 %
1
25.00 %
0
0.00 %
Dorset
PAR-CUL-N
1
298
1.51 %
10
3.36 %
280
93.96 %
13
4.36 %
8
2.68 %
0
0.00 %
PAR-OTHER
1
37
0.19 %
0
0.00 %
36
97.30 %
3
8.11 %
1
2.70 %
0
0.00 %
Gloucs
PAR-CUL-N
1
5
0.03 %
0
0.00 %
5
100.00 %
3
60.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
2
416
2.10 %
56
13.46 %
337
81.01 %
29
6.97 %
22
5.29 %
1
0.24 %
PAR-RECUR
1
55
0.28 %
3
5.45 %
48
87.27 %
4
7.27 %
4
7.27 %
0
0.00 %
Heref
PAR-OTHER
1
104
0.53 %
10
9.62 %
92
88.46 %
4
3.85 %
2
1.92 %
0
0.00 %
PAR-PERSIS
1
71
0.36 %
0
0.00 %
67
94.37 %
4
5.63 %
4
5.63 %
0
0.00 %
PAR-RECUR
5
680
3.44 %
58
8.53 %
594
87.35 %
30
4.41 %
28
4.12 %
0
0.00 %
Shropshire
PAR-OTHER
1
392
1.98 %
31
7.91 %
348
88.78 %
13
3.32 %
13
3.32 %
0
0.00 %
PAR-RECUR
6
1,178
5.95 %
46
3.90 %
1082
91.85 %
56
4.75 %
47
3.99 %
3
0.25 %
Somerset excl N
PAR-CUL-N
9
1,070
5.41 %
28
2.62 %
984
91.96 %
77
7.20 %
57
5.33 %
1
0.09 %
PAR-RECUR
5
509
2.57 %
29
5.70 %
457
89.78 %
28
5.50 %
23
4.52 %
0
0.00 %
Table 2a. Summary by County for October 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Staffs
PAR-CUL-N
1
238
1.20 %
0
0.00 %
230
96.64 %
11
4.62 %
8
3.36 %
0
0.00 %
PAR-RECUR
3
624
3.15 %
25
4.01 %
557
89.26 %
52
8.33 %
42
6.73 %
0
0.00 %
Wiltshire
PAR-CUL-N
4
414
2.09 %
11
2.66 %
375
90.58 %
30
7.25 %
27
6.52 %
1
0.24 %
PAR-RECUR
1
186
0.94 %
8
4.30 %
169
90.86 %
9
4.84 %
8
4.30 %
1
0.54 %
England - Edge
Berks
PAR-NEW-IN
1
62
0.31 %
3
4.84 %
56
90.32 %
4
6.45 %
3
4.84 %
0
0.00 %
PAR-RECUR
1
42
0.21 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Cheshire
PAR-NEW-IN
3
893
4.51 %
22
2.46 %
838
93.84 %
48
5.38 %
33
3.70 %
0
0.00 %
Derbyshire
PAR-NEW-IN
5
1,557
7.87 %
50
3.21 %
1421
91.27 %
95
6.10 %
86
5.52 %
0
0.00 %
PAR-RECUR
2
309
1.56 %
8
2.59 %
247
79.94 %
63
20.39 %
54
17.48 %
0
0.00 %
Hampshire
PAR-NEW-IN
3
706
3.57 %
15
2.12 %
655
92.78 %
45
6.37 %
36
5.10 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
2
442
2.23 %
30
6.79 %
392
88.69 %
25
5.66 %
20
4.52 %
0
0.00 %
Northants
PAR-NEW-IN
1
66
0.33 %
1
1.52 %
64
96.97 %
1
1.52 %
1
1.52 %
0
0.00 %
Oxon
PAR-NEW-IN
8
412
2.08 %
22
5.34 %
354
85.92 %
52
12.62 %
36
8.74 %
0
0.00 %
Warks
PAR-NEW-IN
1
82
0.41 %
2
2.44 %
79
96.34 %
1
1.22 %
1
1.22 %
0
0.00 %
England - LRA
Beds
PAR-NEW-IN
3
347
1.75 %
12
3.46 %
320
92.22 %
15
4.32 %
13
3.75 %
2
0.58 %
Cumbria
PAR-NEW-IN
2
129
0.65 %
1
0.78 %
122
94.57 %
10
7.75 %
6
4.65 %
0
0.00 %
Essex
PAR-NEW-IN
3
247
1.25 %
14
5.67 %
203
82.19 %
19
7.69 %
14
5.67 %
16
6.48 %
Gr Manchester
PAR-NEW-IN
1
35
0.18 %
2
5.71 %
30
85.71 %
4
11.43 %
3
8.57 %
0
0.00 %
England
114
13688
69.17 %
585
4.27 %
12323
90.03 %
947
6.92 %
753
5.50 %
27
0.20 %
Wales - High TB
Carms
PAR-IR-SEV
6
48
0.24 %
11
22.92 %
34
70.83 %
3
6.25 %
3
6.25 %
0
0.00 %
PAR-OTHER
2
21
0.11 %
7
33.33 %
13
61.90 %
2
9.52 %
1
4.76 %
0
0.00 %
PAR-PERSIS
6
2,211
11.17 %
55
2.49 %
2095
94.75 %
85
3.84 %
58
2.62 %
3
0.14 %
PAR-RAPID
8
23
0.12 %
8
34.78 %
14
60.87 %
1
4.35 %
1
4.35 %
0
0.00 %
SER-FLEXI
1
39
0.20 %
3
7.69 %
34
87.18 %
2
5.13 %
2
5.13 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
2
0.01 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
11
0.06 %
2
18.18 %
9
81.82 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
1
25
0.13 %
3
12.00 %
22
88.00 %
1
4.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
174
0.88 %
9
5.17 %
155
89.08 %
12
6.90 %
10
5.75 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
5
11
0.06 %
3
27.27 %
6
54.55 %
3
27.27 %
2
18.18 %
0
0.00 %
PAR-RECUR
1
2
0.01 %
0
0.00 %
1
50.00 %
2
100.00 %
1
50.00 %
0
0.00 %
SER-FLEXI
1
67
0.34 %
5
7.46 %
61
91.04 %
2
2.99 %
1
1.49 %
0
0.00 %
Gwent
PAR-IR-SEV
1
9
0.05 %
0
0.00 %
9
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
230
1.16 %
9
3.91 %
203
88.26 %
26
11.30 %
17
7.39 %
1
0.43 %
PAR-PERSIS
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
3
0.02 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
1
7
0.04 %
1
14.29 %
2
28.57 %
4
57.14 %
4
57.14 %
0
0.00 %
PAR-IR-EX
4
14
0.07 %
2
14.29 %
12
85.71 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
13
79
0.40 %
16
20.25 %
57
72.15 %
7
8.86 %
6
7.59 %
0
0.00 %
PAR-OTHER
3
9
0.05 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
3
47
0.24 %
2
4.26 %
33
70.21 %
13
27.66 %
12
25.53 %
0
0.00 %
PAR-RAPID
17
49
0.25 %
17
34.69 %
27
55.10 %
5
10.20 %
5
10.20 %
0
0.00 %
PAR-RECUR
6
1,353
6.84 %
31
2.29 %
1266
93.57 %
75
5.54 %
53
3.92 %
3
0.22 %
SER-FLEXI
2
76
0.38 %
6
7.89 %
70
92.11 %
0
0.00 %
0
0.00 %
0
0.00 %
Powys
PAR-IR-EX
2
4
0.02 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
13
0.07 %
2
15.38 %
10
76.92 %
1
7.69 %
1
7.69 %
0
0.00 %
PAR-PERSIS
2
9
0.05 %
0
0.00 %
7
77.78 %
2
22.22 %
2
22.22 %
0
0.00 %
PAR-RAPID
4
8
0.04 %
0
0.00 %
8
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
5
0.03 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-RAPID
1
7
0.04 %
2
28.57 %
5
71.43 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
3
31
0.16 %
0
0.00 %
30
96.77 %
1
3.23 %
1
3.23 %
0
0.00 %
PAR-NEW-IN
6
597
3.02 %
9
1.51 %
543
90.95 %
54
9.05 %
45
7.54 %
0
0.00 %
PAR-PERSIS
3
327
1.65 %
7
2.14 %
301
92.05 %
26
7.95 %
19
5.81 %
0
0.00 %
PAR-POSTM
8
61
0.31 %
7
11.48 %
51
83.61 %
4
6.56 %
3
4.92 %
0
0.00 %
PAR-RAPID
4
9
0.05 %
0
0.00 %
8
88.89 %
1
11.11 %
1
11.11 %
0
0.00 %
SER-FLEXI
3
81
0.41 %
2
2.47 %
71
87.65 %
8
9.88 %
8
9.88 %
0
0.00 %
S Glamorgan
PAR-OTHER
1
1
0.01 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for October 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Wales - Low TB
Gwynedd
PAR-NEW-IN
6
97
0.49 %
1
1.03 %
78
80.41 %
31
31.96 %
18
18.56 %
0
0.00 %
PAR-POSTM
7
17
0.09 %
0
0.00 %
15
88.24 %
2
11.76 %
2
11.76 %
0
0.00 %
NE Wales
PAR-IR-EX
1
6
0.03 %
1
16.67 %
4
66.67 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-NEW-IN
5
283
1.43 %
5
1.77 %
259
91.52 %
19
6.71 %
17
6.01 %
2
0.71 %
PAR-POSTM
11
18
0.09 %
1
5.56 %
16
88.89 %
2
11.11 %
1
5.56 %
0
0.00 %
SER-FLEXI
2
17
0.09 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
Wales
162
6102
30.83 %
229
3.75 %
5568
91.25 %
396
6.49 %
296
4.85 %
9
0.15 %
Grand Total
Sum:
276
19790
814
17891
1343
1049
36
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
6
878
0.34 %
66
7.52 %
771
87.81 %
45
5.13 %
38
4.33 %
3
0.34 %
PAR-CUL-P
6
565
0.22 %
31
5.49 %
478
84.60 %
65
11.50 %
56
9.91 %
0
0.00 %
PAR-HERD-S
3
397
0.16 %
38
9.57 %
344
86.65 %
19
4.79 %
15
3.78 %
0
0.00 %
PAR-OTHER
5
317
0.12 %
32
10.09 %
256
80.76 %
41
12.93 %
28
8.83 %
1
0.32 %
PAR-PERSIS
12
1,679
0.66 %
211
12.57 %
1335
79.51 %
148
8.81 %
124
7.39 %
9
0.54 %
PAR-RECUR
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-ANOM
3
26
0.01 %
0
0.00 %
22
84.62 %
3
11.54 %
3
11.54 %
1
3.85 %
Corn & Scilly
PAR-CUL-N
97
8,869
3.48 %
394
4.44 %
7732
87.18 %
872
9.83 %
730
8.23 %
13
0.15 %
PAR-CUL-P
19
3,572
1.40 %
282
7.89 %
3073
86.03 %
245
6.86 %
215
6.02 %
2
0.06 %
PAR-OTHER
1
94
0.04 %
4
4.26 %
83
88.30 %
12
12.77 %
7
7.45 %
0
0.00 %
PAR-PERSIS
10
1,207
0.47 %
41
3.40 %
1112
92.13 %
68
5.63 %
54
4.47 %
0
0.00 %
PAR-RECUR
4
550
0.22 %
73
13.27 %
452
82.18 %
26
4.73 %
25
4.55 %
0
0.00 %
SER-FLEXI
3
593
0.23 %
28
4.72 %
550
92.75 %
20
3.37 %
15
2.53 %
0
0.00 %
SER-OTHER
1
7
0.00 %
0
0.00 %
6
85.71 %
3
42.86 %
1
14.29 %
0
0.00 %
Devon
PAR-CUL-N
342
36,817
14.47 %
2248
6.11 %
32381
87.95 %
2491
6.77 %
2040
5.54 %
148
0.40 %
PAR-CUL-P
14
1,508
0.59 %
86
5.70 %
1298
86.07 %
155
10.28 %
121
8.02 %
3
0.20 %
PAR-NEW-IN
12
1,003
0.39 %
63
6.28 %
842
83.95 %
98
9.77 %
95
9.47 %
3
0.30 %
PAR-OTHER
5
365
0.14 %
27
7.40 %
322
88.22 %
20
5.48 %
16
4.38 %
0
0.00 %
PAR-PERSIS
16
2,804
1.10 %
113
4.03 %
2545
90.76 %
190
6.78 %
143
5.10 %
3
0.11 %
PAR-RECUR
10
964
0.38 %
27
2.80 %
884
91.70 %
73
7.57 %
52
5.39 %
1
0.10 %
SER-FLEXI
5
117
0.05 %
13
11.11 %
99
84.62 %
7
5.98 %
5
4.27 %
0
0.00 %
Dorset
PAR-CUL-N
74
9,002
3.54 %
543
6.03 %
8010
88.98 %
568
6.31 %
444
4.93 %
5
0.06 %
PAR-CUL-P
4
581
0.23 %
56
9.64 %
511
87.95 %
18
3.10 %
14
2.41 %
0
0.00 %
PAR-OTHER
1
37
0.01 %
0
0.00 %
36
97.30 %
3
8.11 %
1
2.70 %
0
0.00 %
PAR-PERSIS
2
405
0.16 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Gloucs
PAR-CUL-N
83
8,234
3.24 %
399
4.85 %
7181
87.21 %
737
8.95 %
617
7.49 %
37
0.45 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
2
189
0.07 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
PAR-OTHER
1
66
0.03 %
2
3.03 %
62
93.94 %
2
3.03 %
2
3.03 %
0
0.00 %
PAR-PERSIS
9
1,901
0.75 %
148
7.79 %
1612
84.80 %
207
10.89 %
138
7.26 %
3
0.16 %
PAR-RECUR
2
248
0.10 %
5
2.02 %
228
91.94 %
17
6.85 %
13
5.24 %
2
0.81 %
Heref
PAR-CUL-N
15
954
0.37 %
48
5.03 %
801
83.96 %
87
9.12 %
79
8.28 %
26
2.73 %
PAR-OTHER
4
724
0.28 %
30
4.14 %
626
86.46 %
73
10.08 %
68
9.39 %
0
0.00 %
PAR-PERSIS
3
181
0.07 %
7
3.87 %
154
85.08 %
20
11.05 %
20
11.05 %
0
0.00 %
PAR-RECUR
6
966
0.38 %
80
8.28 %
847
87.68 %
46
4.76 %
39
4.04 %
0
0.00 %
SER-OTHER
1
52
0.02 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
27
4,557
1.79 %
175
3.84 %
4144
90.94 %
253
5.55 %
220
4.83 %
18
0.39 %
PAR-OTHER
8
1,037
0.41 %
62
5.98 %
941
90.74 %
39
3.76 %
26
2.51 %
8
0.77 %
PAR-RECUR
12
1,809
0.71 %
94
5.20 %
1634
90.33 %
101
5.58 %
78
4.31 %
3
0.17 %
Somerset excl N
PAR-CUL-N
107
11,192
4.40 %
724
6.47 %
9999
89.34 %
615
5.49 %
454
4.06 %
15
0.13 %
PAR-CUL-P
13
1,145
0.45 %
44
3.84 %
1033
90.22 %
79
6.90 %
64
5.59 %
4
0.35 %
PAR-NEW-IN
2
370
0.15 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.18 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
6
969
0.38 %
35
3.61 %
892
92.05 %
59
6.09 %
40
4.13 %
2
0.21 %
PAR-RECUR
6
850
0.33 %
40
4.71 %
777
91.41 %
47
5.53 %
33
3.88 %
0
0.00 %
SER-FLEXI
3
55
0.02 %
9
16.36 %
42
76.36 %
2
3.64 %
2
3.64 %
2
3.64 %
Staffs
PAR-CUL-N
76
10,994
4.32 %
386
3.51 %
9900
90.05 %
824
7.49 %
706
6.42 %
2
0.02 %
PAR-CUL-P
5
471
0.19 %
42
8.92 %
406
86.20 %
39
8.28 %
23
4.88 %
0
0.00 %
PAR-NEW-IN
3
44
0.02 %
2
4.55 %
36
81.82 %
7
15.91 %
6
13.64 %
0
0.00 %
PAR-PERSIS
1
4
0.00 %
0
0.00 %
2
50.00 %
2
50.00 %
2
50.00 %
0
0.00 %
PAR-RECUR
4
632
0.25 %
25
3.96 %
564
89.24 %
53
8.39 %
43
6.80 %
0
0.00 %
Wiltshire
PAR-CUL-N
38
3,778
1.48 %
249
6.59 %
3253
86.10 %
320
8.47 %
267
7.07 %
9
0.24 %
PAR-CUL-P
13
1,096
0.43 %
40
3.65 %
988
90.15 %
62
5.66 %
49
4.47 %
19
1.73 %
PAR-NEW-IN
2
191
0.08 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
11
1,751
0.69 %
76
4.34 %
1573
89.83 %
115
6.57 %
95
5.43 %
7
0.40 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
1
186
0.07 %
8
4.30 %
169
90.86 %
9
4.84 %
8
4.30 %
1
0.54 %
SER-FLEXI
2
172
0.07 %
13
7.56 %
148
86.05 %
1
0.58 %
1
0.58 %
10
5.81 %
Worcs
PAR-CUL-N
2
128
0.05 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
PAR-PERSIS
3
323
0.13 %
45
13.93 %
263
81.42 %
16
4.95 %
15
4.64 %
0
0.00 %
England - Edge
Berks
PAR-CUL-P
2
319
0.13 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
17
1,466
0.58 %
82
5.59 %
1284
87.59 %
111
7.57 %
100
6.82 %
0
0.00 %
PAR-PERSIS
4
247
0.10 %
9
3.64 %
234
94.74 %
7
2.83 %
4
1.62 %
0
0.00 %
PAR-RECUR
1
42
0.02 %
1
2.38 %
38
90.48 %
3
7.14 %
3
7.14 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
12
1,588
0.62 %
43
2.71 %
1488
93.70 %
70
4.41 %
55
3.46 %
2
0.13 %
PAR-PERSIS
4
379
0.15 %
7
1.85 %
353
93.14 %
22
5.80 %
19
5.01 %
0
0.00 %
Cheshire
PAR-CUL-N
66
10,063
3.95 %
326
3.24 %
9282
92.24 %
604
6.00 %
454
4.51 %
1
0.01 %
PAR-NEW-IN
41
6,247
2.45 %
175
2.80 %
5749
92.03 %
404
6.47 %
315
5.04 %
8
0.13 %
PAR-PERSIS
4
348
0.14 %
12
3.45 %
316
90.80 %
22
6.32 %
19
5.46 %
1
0.29 %
Derbyshire
PAR-CUL-N
7
985
0.39 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
126
13,323
5.23 %
555
4.17 %
11562
86.78 %
1347
10.11 %
1138
8.54 %
68
0.51 %
PAR-RECUR
2
309
0.12 %
8
2.59 %
247
79.94 %
63
20.39 %
54
17.48 %
0
0.00 %
E Sussex
PAR-NEW-IN
10
806
0.32 %
82
10.17 %
660
81.89 %
54
6.70 %
47
5.83 %
17
2.11 %
Hampshire
PAR-CUL-N
3
220
0.09 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
16
2,626
1.03 %
77
2.93 %
2366
90.10 %
207
7.88 %
181
6.89 %
2
0.08 %
PAR-PERSIS
1
76
0.03 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
Leics & Rut
PAR-NEW-IN
39
4,519
1.78 %
200
4.43 %
4077
90.22 %
302
6.68 %
241
5.33 %
1
0.02 %
PAR-PERSIS
3
910
0.36 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
43
2,581
1.01 %
86
3.33 %
2347
90.93 %
208
8.06 %
147
5.70 %
1
0.04 %
PAR-PERSIS
3
184
0.07 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
7
772
0.30 %
31
4.02 %
691
89.51 %
72
9.33 %
49
6.35 %
1
0.13 %
Oxon
PAR-CUL-P
1
79
0.03 %
4
5.06 %
71
89.87 %
4
5.06 %
4
5.06 %
0
0.00 %
PAR-NEW-IN
70
5,213
2.05 %
204
3.91 %
4674
89.66 %
399
7.65 %
331
6.35 %
4
0.08 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-PERSIS
12
2,085
0.82 %
89
4.27 %
1902
91.22 %
114
5.47 %
94
4.51 %
0
0.00 %
Warks
PAR-NEW-IN
60
4,463
1.75 %
164
3.67 %
4017
90.01 %
314
7.04 %
272
6.09 %
10
0.22 %
PAR-PERSIS
3
163
0.06 %
10
6.13 %
141
86.50 %
13
7.98 %
12
7.36 %
0
0.00 %
PAR-RECUR
2
128
0.05 %
1
0.78 %
59
46.09 %
5
3.91 %
4
3.13 %
64
50.00 %
England - LRA
Beds
PAR-NEW-IN
7
926
0.36 %
34
3.67 %
817
88.23 %
93
10.04 %
69
7.45 %
6
0.65 %
Cleveland
PAR-NEW-IN
2
194
0.08 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
19
2,798
1.10 %
70
2.50 %
2463
88.03 %
293
10.47 %
254
9.08 %
11
0.39 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.04 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Essex
PAR-NEW-IN
3
247
0.10 %
14
5.67 %
203
82.19 %
19
7.69 %
14
5.67 %
16
6.48 %
Gr Manchester
PAR-NEW-IN
7
575
0.23 %
20
3.48 %
533
92.70 %
28
4.87 %
22
3.83 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.04 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Humberside
PAR-NEW-IN
1
118
0.05 %
4
3.39 %
109
92.37 %
6
5.08 %
5
4.24 %
0
0.00 %
Lancashire
PAR-NEW-IN
8
803
0.32 %
24
2.99 %
734
91.41 %
45
5.60 %
41
5.11 %
4
0.50 %
Lincs
PAR-NEW-IN
18
1,455
0.57 %
51
3.51 %
1291
88.73 %
123
8.45 %
107
7.35 %
6
0.41 %
N Yorks
PAR-NEW-IN
14
526
0.21 %
27
5.13 %
385
73.19 %
121
23.00 %
112
21.29 %
2
0.38 %
Suffolk
PAR-NEW-IN
4
328
0.13 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.08 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
1781
196647
77.26 %
9858
5.01 %
174263
88.62 %
14503
7.38 %
11911
6.06 %
615
0.31 %
Wales - High TB
Carms
PAR-IR-EX
9
48
0.02 %
21
43.75 %
24
50.00 %
2
4.17 %
2
4.17 %
1
2.08 %
PAR-IR-SEV
49
390
0.15 %
90
23.08 %
279
71.54 %
23
5.90 %
21
5.38 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
21
600
0.24 %
102
17.00 %
468
78.00 %
37
6.17 %
28
4.67 %
2
0.33 %
PAR-PERSIS
31
4,139
1.63 %
155
3.74 %
3845
92.90 %
191
4.61 %
135
3.26 %
4
0.10 %
PAR-RAPID
69
204
0.08 %
60
29.41 %
139
68.14 %
5
2.45 %
5
2.45 %
0
0.00 %
PAR-RECUR
8
581
0.23 %
27
4.65 %
530
91.22 %
33
5.68 %
22
3.79 %
2
0.34 %
SER-FLEXI
9
254
0.10 %
27
10.63 %
219
86.22 %
11
4.33 %
7
2.76 %
1
0.39 %
Ceredigion
PAR-IR-EX
7
26
0.01 %
5
19.23 %
21
80.77 %
1
3.85 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-IR-SEV
41
298
0.12 %
75
25.17 %
207
69.46 %
20
6.71 %
14
4.70 %
2
0.67 %
PAR-OTHER
3
64
0.03 %
6
9.38 %
53
82.81 %
7
10.94 %
5
7.81 %
0
0.00 %
PAR-PERSIS
14
1,189
0.47 %
72
6.06 %
1049
88.23 %
78
6.56 %
55
4.63 %
13
1.09 %
PAR-RAPID
38
106
0.04 %
26
24.53 %
75
70.75 %
8
7.55 %
5
4.72 %
0
0.00 %
PAR-RECUR
2
7
0.00 %
1
14.29 %
4
57.14 %
3
42.86 %
2
28.57 %
0
0.00 %
SER-FLEXI
5
214
0.08 %
18
8.41 %
190
88.79 %
7
3.27 %
5
2.34 %
1
0.47 %
Gwent
PAR-IR-EX
2
16
0.01 %
2
12.50 %
14
87.50 %
1
6.25 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
49
0.02 %
1
2.04 %
45
91.84 %
3
6.12 %
3
6.12 %
0
0.00 %
PAR-OTHER
7
389
0.15 %
18
4.63 %
349
89.72 %
33
8.48 %
21
5.40 %
1
0.26 %
PAR-PERSIS
12
485
0.19 %
34
7.01 %
421
86.80 %
31
6.39 %
28
5.77 %
2
0.41 %
PAR-RAPID
8
24
0.01 %
5
20.83 %
19
79.17 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
116
0.05 %
10
8.62 %
100
86.21 %
6
5.17 %
6
5.17 %
0
0.00 %
SER-FLEXI
2
28
0.01 %
5
17.86 %
19
67.86 %
4
14.29 %
4
14.29 %
0
0.00 %
Pembs
PAR-HERD-S
3
17
0.01 %
2
11.76 %
10
58.82 %
6
35.29 %
5
29.41 %
0
0.00 %
PAR-IR-EX
29
157
0.06 %
38
24.20 %
112
71.34 %
7
4.46 %
6
3.82 %
1
0.64 %
PAR-IR-SEV
154
1,236
0.49 %
285
23.06 %
903
73.06 %
58
4.69 %
47
3.80 %
1
0.08 %
PAR-OTHER
35
1,310
0.51 %
82
6.26 %
1170
89.31 %
66
5.04 %
55
4.20 %
3
0.23 %
PAR-PERSIS
101
6,158
2.42 %
255
4.14 %
5639
91.57 %
341
5.54 %
254
4.12 %
10
0.16 %
PAR-RAPID
155
427
0.17 %
132
30.91 %
277
64.87 %
25
5.85 %
18
4.22 %
0
0.00 %
PAR-RECUR
29
4,729
1.86 %
182
3.85 %
4384
92.70 %
197
4.17 %
149
3.15 %
14
0.30 %
SER-FLEXI
53
1,806
0.71 %
148
8.19 %
1603
88.76 %
74
4.10 %
48
2.66 %
7
0.39 %
Powys
PAR-HERD-S
1
10
0.00 %
3
30.00 %
4
40.00 %
3
30.00 %
3
30.00 %
0
0.00 %
PAR-IR-EX
14
82
0.03 %
21
25.61 %
57
69.51 %
6
7.32 %
4
4.88 %
0
0.00 %
PAR-IR-SEV
21
125
0.05 %
27
21.60 %
94
75.20 %
6
4.80 %
4
3.20 %
0
0.00 %
PAR-NEW-IN
1
239
0.09 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
17
939
0.37 %
41
4.37 %
840
89.46 %
65
6.92 %
49
5.22 %
9
0.96 %
PAR-PERSIS
40
2,010
0.79 %
84
4.18 %
1705
84.83 %
232
11.54 %
213
10.60 %
8
0.40 %
PAR-RAPID
28
54
0.02 %
10
18.52 %
42
77.78 %
3
5.56 %
2
3.70 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
12
1,434
0.56 %
57
3.97 %
1312
91.49 %
75
5.23 %
60
4.18 %
5
0.35 %
SER-FLEXI
10
208
0.08 %
20
9.62 %
175
84.13 %
17
8.17 %
13
6.25 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.40 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
2
22
0.01 %
5
22.73 %
17
77.27 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
67
0.03 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.05 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
PAR-RAPID
2
6
0.00 %
1
16.67 %
5
83.33 %
1
16.67 %
0
0.00 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.00 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
10
803
0.32 %
71
8.84 %
698
86.92 %
47
5.85 %
31
3.86 %
3
0.37 %
PAR-PERSIS
6
435
0.17 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
9
18
0.01 %
5
27.78 %
13
72.22 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.09 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.03 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-IR-SEV
1
18
0.01 %
2
11.11 %
16
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
3
106
0.04 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.03 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
2
5
0.00 %
3
60.00 %
2
40.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.05 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.06 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.18 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
2
5
0.00 %
4
80.00 %
1
20.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
33
235
0.09 %
38
16.17 %
187
79.57 %
12
5.11 %
10
4.26 %
0
0.00 %
PAR-NEW-IN
121
10,101
3.97 %
331
3.28 %
9207
91.15 %
682
6.75 %
552
5.46 %
11
0.11 %
PAR-OTHER
5
106
0.04 %
4
3.77 %
95
89.62 %
10
9.43 %
7
6.60 %
0
0.00 %
PAR-PERSIS
26
2,113
0.83 %
56
2.65 %
1955
92.52 %
139
6.58 %
102
4.83 %
0
0.00 %
PAR-POSTM
29
118
0.05 %
8
6.78 %
103
87.29 %
8
6.78 %
7
5.93 %
0
0.00 %
PAR-RAPID
21
69
0.03 %
13
18.84 %
52
75.36 %
5
7.25 %
4
5.80 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
5
267
0.10 %
24
8.99 %
232
86.89 %
13
4.87 %
11
4.12 %
0
0.00 %
SER-FLEXI
23
830
0.33 %
49
5.90 %
752
90.60 %
42
5.06 %
29
3.49 %
0
0.00 %
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
1
3
0.00 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
20
1,884
0.74 %
53
2.81 %
xx-xxxx-xxxx
89.28 %
163
8.65 %
146
7.75 %
3
0.16 %
PAR-PERSIS
1
14
0.01 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-POSTM
7
18
0.01 %
2
11.11 %
15
83.33 %
2
11.11 %
1
5.56 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
5
196
0.08 %
8
4.08 %
169
86.22 %
23
11.73 %
18
9.18 %
1
0.51 %
S Glamorgan
PAR-OTHER
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
3
9
0.00 %
5
55.56 %
4
44.44 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
221
0.09 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-IR-SEV
1
9
0.00 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
3
126
0.05 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
57
0.02 %
1
1.75 %
55
96.49 %
1
1.75 %
1
1.75 %
0
0.00 %
PAR-IR-SEV
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
43
2,009
0.79 %
55
2.74 %
1805
89.85 %
190
9.46 %
145
7.22 %
4
0.20 %
PAR-OTHER
2
51
0.02 %
0
0.00 %
50
98.04 %
2
3.92 %
1
1.96 %
0
0.00 %
PAR-POSTM
13
31
0.01 %
0
0.00 %
28
90.32 %
4
12.90 %
3
9.68 %
0
0.00 %
PAR-RAPID
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
4
65
0.03 %
7
10.77 %
53
81.54 %
6
9.23 %
5
7.69 %
0
0.00 %
NE Wales
PAR-HERD-S
4
111
0.04 %
9
8.11 %
99
89.19 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-IR-EX
1
6
0.00 %
1
16.67 %
4
66.67 %
1
16.67 %
1
16.67 %
0
0.00 %
PAR-NEW-IN
43
3,000
1.18 %
139
4.63 %
2636
87.87 %
260
8.67 %
218
7.27 %
7
0.23 %
PAR-OTHER
4
152
0.06 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.02 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
PAR-POSTM
31
68
0.03 %
3
4.41 %
63
92.65 %
6
8.82 %
2
2.94 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
9
814
0.32 %
53
6.51 %
721
88.57 %
46
5.65 %
40
4.91 %
0
0.00 %
Wales
1590
56704
22.28 %
3305
5.83 %
50432
88.94 %
3588
6.33 %
2835
5.00 %
132
0.23 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.07 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
7
846
0.33 %
113
13.36 %
666
78.72 %
65
7.68 %
58
6.86 %
9
1.06 %
PAR-PERSIS
1
108
0.04 %
7
6.48 %
97
89.81 %
7
6.48 %
4
3.70 %
0
0.00 %
Dumfries & G
PAR-NEW-IN
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.00 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
14
1166
0.46 %
136
11.66 %
947
81.22 %
86
7.38 %
73
6.26 %
10
0.86 %
Grand Total
Sum:
3385 254517
13299
225642
18177
14819
757
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol October 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
111
13348
97.5%
567
4.2%
12014
90.0%
929
7.0%
740
5.5%
27
0.2%
Serial/Extended
3
340
2.5%
18
5.3%
309
90.9%
18
5.3%
13
3.8%
0
0.0 %
Total
114
13688
100.0%
585
4.3%
12323
90.0%
947
6.9%
753
5.5%
27
0.2%
Scotland
Parallel
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Wales
Parallel
153
5822
95.4%
212
3.6%
5317
91.3%
383
6.6%
284
4.9%
9
0.2%
Serial/Extended
9
280
4.6%
17
6.1%
251
89.6%
13
4.6%
12
4.3%
0
0.0 %
Total
162
6102
100.0%
229
3.8%
5568
91.2%
396
6.5%
296
4.9%
9
0.1%
GB
Parallel
264
19170
96.9%
779
4.1%
17331
90.4%
1312
6.8%
1024
5.3%
36
0.2%
Serial/Extended
12
620
3.1%
35
5.6%
560
90.3%
31
5.0%
25
4.0%
0
0.0 %
Total
276
19790
100.0%
814
4.1%
17891
90.4%
1343
6.8%
1049
5.3%
36
0.2%
114.00
162.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
1763 195625
99.5%
9795
5.0% 173345
88.6%
14466
7.4%
11883
6.1%
602
0.3%
Serial/Extended
18
1022
0.5%
63
6.2%
918
89.8%
37
3.6%
28
2.7%
13
1.3%
Total
1781 196647
100.0%
9858
5.0% 174263
88.6%
14503
7.4%
11911
6.1%
615
0.3%
Scotland
Parallel
13
1162
99.7%
136
11.7%
944
81.2%
86
7.4%
73
6.3%
9
0.8%
Serial/Extended
1
4
0.3%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
14
1166
100.0%
136
11.7%
947
81.2%
86
7.4%
73
6.3%
10
0.9%
Wales
Parallel
1468
52252
92.1%
2950
5.6%
46501
89.0%
3372
6.5%
2679
5.1%
122
0.2%
Serial/Extended
122
4452
7.9%
355
8.0%
3931
88.3%
216
4.9%
156
3.5%
10
0.2%
Total
1590
56704
100.0%
3305
5.8%
50432
88.9%
3588
6.3%
2835
5.0%
132
0.2%
GB
Parallel
xx-xxxx-xxxx
97.8% 12881
5.2% 220790
88.7%
17924
7.2%
14635
5.9%
733
0.3%
Serial/Extended
141
5478
2.2%
418
7.6%
4852
88.6%
253
4.6%
184
3.4%
24
0.4%
Total
3385 254517
100.0% 13299
5.2% 225642
88.7%
18177
7.1%
14819
5.8%
757
0.3%
#######
#######
14.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
11
613
3.1%
35
5.7%
554
90.4%
28
4.6 %
24
3.9 %
0
Parallel - Herd or Group Slaughter
1
7
0.0%
1
14.3%
2
28.6%
4
57.1 %
4
57.1 %
0
Parallel - IRs in Exit Strategy for persistent her
8
26
0.1%
3
11.5%
22
84.6%
1
3.8 %
1
3.8 %
0
Parallel New Infection
50
5955
30.1%
189
3.2%
5414
90.9%
423
7.1 %
332
5.6 %
20
0.3 %
Parallel - new infection cull area
26
2464
12.5%
71
2.9%
2242
91.0%
188
7.6 %
149
6.0 %
2
0.1 %
Parallel - Other
16
1003
5.1%
84
8.4%
868
86.5%
77
7.7 %
49
4.9 %
2
0.2 %
Parallel Persistent Infection
28
3982
20.1%
144
3.6%
3668
92.1%
219
5.5 %
166
4.2 %
4
0.1 %
Parallel - persistent infection cull area
1
5
0.0%
1
20.0%
4
80.0%
2
40.0 %
0
0
Parallel – post-mvt test coupled with IDEXX an
26
96
0.5%
8
8.3%
82
85.4%
8
8.3 %
6
6.3 %
0
Parallel - Rapid Testing Two-times IRs
40
110
0.6%
30
27.3%
71
64.5%
10
9.1 %
9
8.2 %
0
Parallel - Recurrent breakdowns
42
5331
26.9%
217
4.1%
4809
90.2%
368
6.9 %
297
5.6 %
8
0.2 %
Parallel - severe IRs - in persistent herds
26
191
1.0%
31
16.2%
149
78.0%
12
6.3 %
11
5.8 %
0
Serial - Other
1
7
0.0%
0
0.0 %
6
85.7%
3
42.9 %
1
14.3 %
0
Total
276
19790
100.0%
814
4.1%
17891
90.4%
1343
6.8 %
1049
5.3 %
36
0.2 %
Table 4a. Summaryof IFN Gamma results by submission reason (October 2021)
England - HRA
Extended Flexible IFN-GAMMA test
2
333
1.7%
18
5.4%
303
91.0%
15
4.5 %
12
3.6 %
0
Parallel - new infection cull area
26
2464
12.5%
71
2.9%
2242
91.0%
188
7.6 %
149
6.0 %
2
0.1 %
Parallel - Other
6
717
3.6%
64
8.9%
621
86.6%
48
6.7 %
31
4.3 %
1
0.1 %
Parallel Persistent Infection
12
1213
6.1%
71
5.9%
1076
88.7%
81
6.7 %
65
5.4 %
1
0.1 %
Parallel - persistent infection cull area
1
5
0.0%
1
20.0%
4
80.0%
2
40.0 %
0
0
Parallel - Recurrent breakdowns
30
3620
18.3%
177
4.9%
3252
89.8%
225
6.2 %
186
5.1 %
5
0.1 %
Serial - Other
1
7
0.0%
0
0.0 %
6
85.7%
3
42.9 %
1
14.3 %
0
England - HRA
78
8359
42.2%
402
4.8%
7504
89.8%
562
6.7 %
444
5.3 %
9
0.1 %
England - Edge
Parallel New Infection
24
4220
21.3%
145
3.4%
3859
91.4%
271
6.4 %
216
5.1 %
0
Parallel - Recurrent breakdowns
3
351
1.8%
9
2.6%
285
81.2%
66
18.8 %
57
16.2 %
0
England - Edge
27
4571
23.1%
154
3.4%
4144
90.7%
337
7.4 %
273
6.0 %
0
England - LRA
Parallel New Infection
9
758
3.8%
29
3.8%
675
89.1%
48
6.3 %
36
4.7 %
18
2.4 %
England - LRA
9
758
3.8%
29
3.8%
675
89.1%
48
6.3 %
36
4.7 %
18
2.4 %
Wales - High TB
Extended Flexible IFN-GAMMA test
4
182
0.9%
14
7.7%
165
90.7%
4
2.2 %
3
1.6 %
0
Parallel - Herd or Group Slaughter
1
7
0.0%
1
14.3%
2
28.6%
4
57.1 %
4
57.1 %
0
Parallel - IRs in Exit Strategy for persistent her
7
20
0.1%
2
10.0%
18
90.0%
0
0
0
Parallel - Other
9
285
1.4%
20
7.0%
246
86.3%
29
10.2 %
18
6.3 %
1
0.4 %
Parallel Persistent Infection
13
2442
12.3%
66
2.7%
2291
93.8%
112
4.6 %
82
3.4 %
3
0.1 %
Parallel - Rapid Testing Two-times IRs
35
94
0.5%
28
29.8%
58
61.7%
9
9.6 %
8
8.5 %
0
Parallel - Recurrent breakdowns
9
1360
6.9%
31
2.3%
1272
93.5%
77
5.7 %
54
4.0 %
3
0.2 %
Parallel - severe IRs - in persistent herds
23
160
0.8%
31
19.4%
119
74.4%
11
6.9 %
10
6.3 %
0
Wales - High TB
101
4550
23.0%
193
4.2%
4171
91.7%
246
5.4 %
179
3.9 %
7
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
81
0.4%
2
2.5%
71
87.7%
8
9.9 %
8
9.9 %
0
Parallel New Infection
6
597
3.0%
9
1.5%
543
91.0%
54
9.0 %
45
7.5 %
0
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
3
327
1.7%
7
2.1%
301
92.0%
26
8.0 %
19
5.8 %
0
Parallel – post-mvt test coupled with IDEXX an
8
61
0.3%
7
11.5%
51
83.6%
4
6.6 %
3
4.9 %
0
Parallel - Rapid Testing Two-times IRs
5
16
0.1%
2
12.5%
13
81.3%
1
6.3 %
1
6.3 %
0
Parallel - severe IRs - in persistent herds
3
31
0.2%
0
0.0 %
30
96.8%
1
3.2 %
1
3.2 %
0
Wales - Intermediate TB
29
1114
5.6%
27
2.4%
1010
90.7%
94
8.4 %
77
6.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
17
0.1%
1
5.9%
15
88.2%
1
5.9 %
1
5.9 %
0
Parallel - IRs in Exit Strategy for persistent her
1
6
0.0%
1
16.7%
4
66.7%
1
16.7 %
1
16.7 %
0
Parallel New Infection
11
380
1.9%
6
1.6%
337
88.7%
50
13.2 %
35
9.2 %
2
0.5 %
Parallel – post-mvt test coupled with IDEXX an
18
35
0.2%
1
2.9%
31
88.6%
4
11.4 %
3
8.6 %
0
Wales - Low TB
32
438
2.2%
9
2.1%
387
88.4%
56
12.8 %
40
9.1 %
2
0.5 %

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
135
5389
2.1%
418
7.8%
4770
88.5%
246
4.6 %
179
3.3 %
22
0.4 %
Parallel - Herd or Group Slaughter
21
1631
0.6%
98
6.0%
1463
89.7%
78
4.8 %
65
4.0 %
5
0.3 %
Parallel - IRs in Exit Strategy for persistent herds
65
344
0.1%
92
26.7%
237
68.9%
18
5.2 %
13
3.8 %
2
0.6 %
Parallel New Infection
798
72475
28.5%
2847
3.9%
64495
89.0%
5903
8.1 %
4924
6.8 %
209
0.3 %
Parallel - new infection cull area
945 106706
41.9%
5639
5.3%
94604
88.7%
7555
7.1 %
6182
5.8 %
281
0.3 %
Parallel - Other
137
7695
3.0%
513
6.7%
6733
87.5%
517
6.7 %
420
5.5 %
29
0.4 %
Parallel Persistent Infection
345
32519
12.8%
1551
4.8%
29223
89.9%
2154
6.6 %
xx-xxxx-xxxx
5.2 %
64
0.2 %
Parallel - persistent infection cull area
79
9525
3.7%
623
6.5%
8298
87.1%
685
7.2 %
558
5.9 %
46
0.5 %
Parallel – post-mvt test coupled with IDEXX anti
80
235
0.1%
13
5.5%
209
88.9%
20
8.5 %
13
5.5 %
0
Parallel - Rapid Testing Two-times IRs
341
929
0.4%
261
28.1%
634
68.2%
47
5.1 %
34
3.7 %
0
Parallel - Recurrent breakdowns
122
14594
5.7%
720
4.9%
13134
90.0%
825
5.7 %
646
4.4 %
94
0.6 %
Parallel - severe IRs - in persistent herds
311
2386
0.9%
524
22.0%
1760
73.8%
122
5.1 %
99
4.1 %
3
0.1 %
Serial - Other
2
59
0.0%
0
0.0 %
57
96.6%
4
6.8 %
2
3.4 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
3385 254517
100.0%
13299
5.2%
225642
88.7%
18177
7.1 %
14819
5.8 %
757
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
3
26
0.0%
0
0.0 %
22
84.6%
3
11.5 %
3
11.5 %
1
3.8 %
Extended Flexible IFN-GAMMA test
13
937
0.4%
63
6.7%
839
89.5%
30
3.2 %
23
2.5 %
12
1.3 %
Parallel - Herd or Group Slaughter
3
397
0.2%
38
9.6%
344
86.6%
19
4.8 %
15
3.8 %
0
Parallel New Infection
19
1608
0.6%
131
8.1%
1347
83.8%
133
8.3 %
127
7.9 %
3
0.2 %
Parallel - new infection cull area
867
95403
37.5%
5244
5.5%
84284
88.3%
6815
7.1 %
5597
5.9 %
278
0.3 %
Parallel - Other
27
3107
1.2%
179
5.8%
2715
87.4%
225
7.2 %
204
6.6 %
9
0.3 %
Parallel Persistent Infection
73
11224
4.4%
684
6.1%
9870
87.9%
843
7.5 %
646
5.8 %
24
0.2 %
Parallel - persistent infection cull area
76
9127
3.6%
616
6.7%
7935
86.9%
671
7.4 %
548
6.0 %
28
0.3 %
Parallel - Recurrent breakdowns
46
6206
2.4%
352
5.7%
5556
89.5%
372
6.0 %
291
4.7 %
7
0.1 %
Serial - Other
2
59
0.0%
0
0.0 %
57
96.6%
4
6.8 %
2
3.4 %
0
England - Edge
Parallel New Infection
441
43604
17.1%
xx-xxxx-xxxx
3.9%
38915
89.2%
3488
8.0 %
2876
6.6 %
114
0.3 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - new infection cull area
77
11285
4.4%
395
3.5%
10314
91.4%
730
6.5 %
575
5.1 %
1
0.0 %
Parallel Persistent Infection
34
4392
1.7%
158
3.6%
4019
91.5%
253
5.8 %
213
4.8 %
2
0.0 %
Parallel - persistent infection cull area
3
398
0.2%
7
1.8%
363
91.2%
14
3.5 %
10
2.5 %
18
4.5 %
Parallel - Recurrent breakdowns
5
479
0.2%
10
2.1%
344
71.8%
71
14.8 %
61
12.7 %
64
13.4 %
England - LRA
Parallel New Infection
91
8377
3.3%
282
3.4%
7333
87.5%
822
9.8 %
710
8.5 %
52
0.6 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
1781 196647
77.3%
9858
5.0%
174263
88.6%
14503
7.4 %
11911
6.1 %
615
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
79
2510
1.0%
218
8.7%
2206
87.9%
113
4.5 %
77
3.1 %
9
0.4 %
Parallel - Herd or Group Slaughter
9
1057
0.4%
49
4.6%
958
90.6%
52
4.9 %
45
4.3 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
61
329
0.1%
87
26.4%
228
69.3%
17
5.2 %
12
3.6 %
2
0.6 %
Parallel New Infection
2
245
0.1%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
85
3369
1.3%
249
7.4%
2941
87.3%
215
6.4 %
164
4.9 %
15
0.4 %
Parallel Persistent Infection
200
14117
5.5%
602
4.3%
12788
90.6%
878
6.2 %
690
4.9 %
37
0.3 %
Parallel - Rapid Testing Two-times IRs
301
823
0.3%
234
28.4%
559
67.9%
42
5.1 %
30
3.6 %
0
Parallel - Recurrent breakdowns
54
6867
2.7%
277
4.0%
6330
92.2%
314
4.6 %
239
3.5 %
21
0.3 %
Parallel - severe IRs - in persistent herds
274
2120
0.8%
483
22.8%
1545
72.9%
110
5.2 %
89
4.2 %
3
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
30
1063
0.4%
77
7.2%
951
89.5%
51
4.8 %
34
3.2 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
3
9
0.0%
4
44.4%
5
55.6%
0
0
0
Parallel New Infection
147
12578
4.9%
406
3.2%
11394
90.6%
911
7.2 %
759
6.0 %
19
0.2 %
Parallel - Other
19
1016
0.4%
81
8.0%
887
87.3%
67
6.6 %
44
4.3 %
4
0.4 %
Parallel Persistent Infection
35
2635
1.0%
98
3.7%
2409
91.4%
173
6.6 %
128
4.9 %
0
Parallel – post-mvt test coupled with IDEXX ant
36
136
0.1%
10
7.4%
118
86.8%
10
7.4 %
8
5.9 %
0
Parallel - Rapid Testing Two-times IRs
36
102
0.0%
26
25.5%
72
70.6%
5
4.9 %
4
3.9 %
0
Parallel - Recurrent breakdowns
17
1042
0.4%
81
7.8%
904
86.8%
68
6.5 %
55
5.3 %
2
0.2 %
Parallel - severe IRs - in persistent herds
36
265
0.1%
41
15.5%
214
80.8%
12
4.5 %
10
3.8 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
13
879
0.3%
60
6.8%
774
88.1%
52
5.9 %
45
5.1 %
0
Parallel - Herd or Group Slaughter
7
168
0.1%
10
6.0%
154
91.7%
6
3.6 %
4
2.4 %
0
Parallel - IRs in Exit Strategy for persistent herd
1
6
0.0%
1
16.7%
4
66.7%
1
16.7 %
1
16.7 %
0
Parallel New Infection
86
5009
2.0%
194
3.9%
4441
88.7%
450
9.0 %
363
7.2 %
11
0.2 %
Parallel - Other
6
203
0.1%
4
2.0%
190
93.6%
10
4.9 %
8
3.9 %
1
0.5 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %
Parallel – post-mvt test coupled with IDEXX ant
44
99
0.0%
3
3.0%
91
91.9%
10
10.1 %
5
5.1 %
0

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - Rapid Testing Two-times IRs
4
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel - severe IRs - in persistent herds
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
1590
56704
22.3%
3305
5.8%
50432
88.9%
3588
6.3 %
2835
5.0 %
132
0.2 %
Scotland - Scotland
Parallel New Infection
12
1054
0.4%
129
12.2%
847
80.4%
79
7.5 %
69
6.5 %
9
0.9 %
Parallel Persistent Infection
1
108
0.0%
7
6.5%
97
89.8%
7
6.5 %
4
3.7 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Scotland
14
1166
0.5%
136
11.7%
947
81.2%
86
7.4 %
73
6.3 %
10
0.9 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 November
347
26584
200
1359
29
0
0
0
0
105
3
0
7
1
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
2
70
2
0
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
0
84
5
12
March
419
36344
256
1867
24
0
0
0
0
77
7
0
8
0
1657
1
93
0
0
April
367
28108
211
1571
35
0
0
0
0
105
3
0
13
0
1338
0
75
1
0
May
357
27182
214
1564
3
65
0
0
0
0
130
3
0
9
1
1220
0
131
1
1
June
355
27828
208
1314
1
19
0
0
0
0
105
1
0
4
3
1083
5
92
0
1
July
298
24841
164
1077
30
0
0
0
0
61
0
0
4
4
936
2
38
0
2
August
312
17920
167
912
3
32
0
0
0
0
35
0
2
7
0
749
10
70
0
4
September
274
15805
130
890
40
0
0
0
0
95
0
8
0
0
720
22
0
0
5
October
276
19790
140
814
273
290
0
0
0
0
10
0
3
0
0
83
1
0
0
0
Totals (last
12 months)
4056
305247
2344
15886
283
692
0
0
0
0
1036
24
13
87
9
12666
43
838
10
25
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2109
235582
1379
11839
563
0
0
0
0
894
12
7
51
4
9891
6
76
0
6
Scotland
28
2915
19
190
0
0
0
0
0
50
2
0
6
0
53
7
56
3
12
Wales
1919
66750
946
3857
129
0
0
0
0
92
10
6
30
5
2722
30
706
7
7
Totals
4056
305247
2344
15886
692
0
0
0
0
1036
24
13
87
9
12666
43
838
10
25
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
134
164
145
167
144
130
136
140
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
23382
1077
16764
912
14734
889
18674
814
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
9
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
5
10
15
20
25
30
35
40
45
50
Wiltshire
Warks
Staffs
Somerset excl N
Shropshire
S Glamorgan
Powys
Pembs
Oxon
Northants
NE Wales
Leics & Rut
Heref
Hampshire
Gwynedd
Gwent
Gr Manchester
Gloucs
Essex
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cheshire
Ceredigion
Carms
Berks
Beds
Avon
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during October 2021 (% submission per
County)
County
%Resampled %Retested
Avon
13.73 %
21.57 %
Beds
3.75 %
4.32 %
Berks
5.77 %
6.73 %
Carms
2.77 %
3.96 %
Ceredigion
4.79 %
6.85 %
Cheshire
3.70 %
5.38 %
Corn & Scilly
6.61 %
8.56 %
Cumbria
4.65 %
7.75 %
Derbyshire
7.50 %
8.47 %
Devon
7.10 %
8.99 %
Dorset
2.69 %
4.78 %
Essex
5.67 %
7.69 %
Gloucs
5.46 %
7.56 %
Gr Manchester
8.57 %
11.43 %
Gwent
7.00 %
10.70 %
Gwynedd
17.54 %
28.95 %
Hampshire
5.10 %
6.37 %
Heref
3.98 %
4.44 %
Leics & Rut
4.52 %
5.66 %
NE Wales
6.78 %
8.18 %
Northants
1.52 %
1.52 %
Oxon
8.74 %
12.62 %
Pembs
4.90 %
6.36 %
Powys
7.69 %
7.69 %
S Glamorgan
Shropshire
3.82 %
4.39 %
Somerset excl
5.07 %
6.65 %
Staffs
5.80 %
7.31 %
Warks
1.22 %
1.22 %
Wiltshire
5.83 %
6.50 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
13517
585
4.3%
13688
585
4.3%
Wales
5971
229
3.8%
6102
229
3.8%
GB
19488
814
4.2%
19790
814
4.1%
Table 6a (i): Number of animals and submissions tested in October 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
184880
9855
5.3%
196647
9858
5.0%
Wales
52896
3304
6.2%
56704
3305
5.8%
Scotland
579
136
23.5%
1166
136
11.7%
GB
238345
13295
5.6%
254517
13299
5.2%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
19191
801
4.2%
19353
801
4.1%
Wales
7243
397
5.5%
7315
397
5.4%
GB
26434
1198
4.5%
26668
1198
4.5%
Table 6a (ii): Number of animals and submissions tested in October 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of October
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
173495
6875
4.0%
182831
6875
3.8%
Wales
42989
2564
6.0%
46356
2565
5.5%
Scotland
3635
107
2.9%
4530
107
2.4%
GB
220118
9546
4.3%
233717
9547
4.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Cornwall and Isles of Scilly
PRV-SP
1
1
0
0
Dorset
PRV-SE
1
18
5
27.78 %
Total
2
19
5
26.32 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples October 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Berkshire
PRV-SP
1
3
0
0
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
2
2
0
0
Derbyshire
PRV-SP
2
2
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
2
3
0
0
Dorset
PRV-SE
1
18
5
27.78 %
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
6
326
11
3.37 %
PRV-SP
3
5
0
0
Staffordshire
PRV-SP
2
2
0
0
Wiltshire
PRV-SE
1
10
0
0
PRV-SP
1
3
0
0
Worcestershire
PRV-SE
1
1
0
0
PRV-SP
2
3
0
0
Total
31
455
20
4.40 %
Isle of Man
SER-IOM
7
8
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
182
4
2.2%
Devon
BOV-P
1
7
0
0
England
2
189
4
2.1%
Wales
Carmarthenshire
BOV-IR-C
5
52
16
30.8%
Ceredigion
BOV-IR-C
5
70
14
20.0%
BOV-IR-EX
2
7
3
42.9%
BOV-P
1
63
2
3.2%
North East Wales
BOV-IR-C
2
27
2
7.4%
BOV-OTHER
1
1
1
100.0%
BOV-P
3
202
10
5.0%
BOV-POSTM
14
24
4
16.7%
North West Wales
BOV-POSTM
2
11
2
18.2%
Pembrokeshire
BOV-IR-C
10
122
13
10.7%
Powys
BOV-IR-C
2
16
4
25.0%
BOV-POSTM
1
3
0
0
South Wales
BOV-IR-C
1
12
2
16.7%
Wales
49
610
73
12.0%
All GB
51
799
77
9.6 %
Table 8a Number of Cattle Antibody Tests October 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
3
594
21
3.5%
Devon
1
95
6
6.3%
BOV-P
2
47
0
0
Staffordshire
BOV-P
1
30
1
3.3%
Wiltshire
BOV-P
1
189
8
4.2%
England
8
955
36
3.8%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
39
433
103
23.8%
BOV-IR-EX
6
45
13
28.9%
BOV-OTHER
3
80
4
5.0%
BOV-P
2
87
3
3.4%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
35
346
61
17.6%
BOV-IR-EX
4
21
6
28.6%
BOV-OTHER
6
240
16
6.7%
BOV-P
2
79
2
2.5%
North East Wales
2
24
6
25.0%
BOV-IR-C
27
265
40
15.1%
BOV-IR-EX
2
5
1
20.0%
BOV-OTHER
5
150
5
3.3%
BOV-P
15
1,388
54
3.9%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-POSTM
46
106
26
24.5%
OTHER
1
48
4
8.3%
North West Wales
BOV-P
1
42
0
0
BOV-POSTM
4
14
4
28.6%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
128
1,392
246
17.7%
BOV-IR-EX
16
123
27
22.0%
BOV-OTHER
18
855
48
5.6%
BOV-P
14
779
31
4.0%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
23
169
33
19.5%
BOV-IR-EX
3
45
9
20.0%
BOV-OTHER
5
227
30
13.2%
BOV-P
3
54
3
5.6%
BOV-POSTM
4
8
1
12.5%
South Wales
4
14
5
35.7%
BOV-IR-C
9
77
7
9.1%
BOV-IR-EX
1
21
1
4.8%
BOV-P
2
87
1
1.1%
Wales
460
7,564
834
11.0%
All GB
468
8,519
870
10.2 %