Monthly report: Surveillance Project SB4008 IFNg Tests for Bovine Tuberculosis - June 2021
Source: environment.data.gov.uk (2022-01-18)

Interferon-Gamma Testing for Bovine Tuberculosis - Monthly Reports from April 2016. These monthly reports summarise the gamma tests completed / reported during that calendar month. The report also summarises tests for the whole calendar year to the end of the current month.
While all effort is made to ensure the accuracy of the test data, small errors can occur and later corrected in subsequent reports. 

Cattle interferon-gamma (IFNg) tests (and test outcome) carried out between 1st Jan and 31st Dec 2009. Tests are listed by country (England, Wales, Scotland) and by Animal Health Office (AHDO) and the reason for the test. Reports contain test data for the month, the calendar year to date and total tests since rollout (23rd Oct 2006). 

Please note: this dataset consists of 12 monthly reports. Data for this report was compiled from field staff inputs and laboratory test information. Subsequent minor data amendments may have been made after the report was originally compiled, and these corrections would appear in the following month’s report. Each report was checked and deemed correct at the time of release. Attribution statement:

Monthly Report:
Surveillance Project
SB4008 IFNg (TC0651 and
TC0751) & Antibody
(TC0077) tests for bovine
tuberculosis
Number 178
Report Period 1st - 30th June 2021
Issued 22 July 2021

Operational notes on terminology, definitions, re-test and re-samples test
outcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which is
subsequently submitted for testing. It is generally assumed that the number of samples and
the number of animals are analogous as any duplicate sample blood tube from the same
animal will not be tested. However, minor discrepancies may occur where the same animal is
tested more than once due to a request for a resample or the animal is tested twice under
different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.
Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it
is usually sent as 1 submission). It is generally assumed that the number of submissions and
the number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on a
new ELISA plate. Each sample can be retested only once. A retest is not a reportable test
outcome.
• Resamples are where a sample has been retested and failed a second time, so that the lab
requests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: blood
collected into wrong type of vacutainer, samples that have not been maintained at the
appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood
that is extensively clotted (small clots are OK), samples received after 4pm on the day after
sample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of
the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may
indicate compromised blood quality as a result of collection/transportation conditions or due
the animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of the
background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)
may indicate a laboratory procedures problem (normally resolved during re-test) or that an
animal has unusually high background levels of IFN-g.

1. Interferon-gamma test:
Submission Reason
Explanation
Mandatory?
Possible Herd Slaughter
(PAR-HERD-S)
Parallel interferon-gamma blood testing of skin
test negative cattle to inform whole or partial
herd slaughters decisions
YES, if APHA are
contemplating a herd
slaughter
Persistent TB breakdowns
(OTF status withdrawn)
(PAR-P-CONF) N.B.
PAR-PERSIS from 1st April
2017
Parallel blood testing of skin test-negative cattle
in persistently infected herds that have failed to
resolve by repeated short-interval skin testing
and fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence
(PAR-LOW-IN) N.B.
PAR-NEW-IN from 1st April
2017
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new herd breakdown as soon as possible after
confirmation of TB.
YES in Area of Low
Incidence
PAR-RAPID
Parallel blood testing of two-times IRs identified
under the severe interpretation of the skin test
used in Wales.
YES (Wales only)
PAR-IR-SEV
Parallel blood testing of all severe IRs identified
in persistent breakdown herds only (coupled
with Idexx antibody testing)
YES (Wales only)
PAR-IR-EX
Parallel blood testing of all severe IRs in the
Exit Strategy for persistent herds (coupled with
Idexx antibody testing),
and also for new breakdown herds in ITBAN
(Intermediate TB Area North) (not coupled with
IDEXX testing).
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise the
probability of removing all infected cattle in a
new breakdown as soon as possible after
confirmation of TB where herd is situated in
badger control areas of England that have
completed at least 2 effective culls.
YES (in eligible badger
control areas of England)
PAR-CUL-P
Parallel blood testing to maximise the
probability of removing all infected cattle in a
persistent confirmed breakdown where herd is
situated within badger control areas of England
that have completed at least 2 effective culls
NO (APHA discretion)
PAR-RECUR
Parallel blood testing of skin test-negative cattle
in breakdowns herds occurring within 6 months
of lifting restrictions after a previous breakdown
NO (APHA discretion -
Wales only)
PAR-POSTM
Post movement blood test of cattle (coupled
with Idexx antibody testing)
Yes (currently Wales
ITBAN only)
PAR-OTHER
Other parallel blood testing not covered in any
of the other scenarios
NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skin
test reactors and/or IRs in unconfirmed TB
breakdown herds to clarify their infection status
where there is evidence of non-specific
sensitisation to bovine tuberculin (the
“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud
(SER-FRAUD)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Test Criteria

Anomalous reaction
(SER-ANOM)
Modified serial blood test of suspected
fraudulent reactors to the skin test (animals with
abnormal skin swellings), in confirmed or
unconfirmed TB incidents.
NO (APHA discretion to
firm up or rule out any
suspicion of fraud and
support any
investigations)
Serial Other
(SER-OTHER)
Serial test – other reasons
NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible test
readout in a confirmed M. bovis-infected herd
where Johne's (M. a. paratuberculosis) infection
or vaccination is suspected to be interferring
with M. bovis infection detection
NO (APHA discretion)
2. IDEXX antibody test:
Submission Reason
Explanation
Mandatory?
BOV-P
Supplementary antibody testing within
persistent infected herds
NO (APHA discretion -
Eng & Wales)
BOV-OTHER
Antibody testing for reasons not covered by the
above
NO (APHA discretion -
Eng & Wales)
BOV-IR-EX
Compulsory antibody testing of IRs as part of
Exit Strategy (coupled with interferon-gamma
testing)
YES (Wales only)
BOV-IR-C
Compulsory antibody testing of severe IRs
(coupled interferon-gamma testing)
YES (Wales only)
BOV-POSTM
Compulsory post-movement antibody test of
cattle (coupled with interferon-gamma testing)
YES (Wales only)

Num
samples
June 2021
%
Total 2021
%
Submissions
355
2225
Samples
Total (%)
27828
100.00 %
176161
100.00 %
England
22225
79.87 %
136336
77.39 %
Wales
5412
19.45 %
38767
22.01 %
Scotland
191
0.69 %
1058
0.60 %
Parallel Tests
PAR-CUL-N
14062
50.53 %
80829
45.88 %
PAR-CUL-P
927
3.33 %
6002
3.41 %
PAR-HERD-S
129
0.46 %
1217
0.69 %
PAR-IR-EX
85
0.31 %
231
0.13 %
PAR-IR-SEV
177
0.64 %
1091
0.62 %
PAR-NEW-IN
7282
26.17 %
52776
29.96 %
PAR-OTHER
75
0.27 %
5426
3.08 %
PAR-PERSIS
4283
15.39 %
19176
10.89 %
PAR-POSTM
10
0.04 %
10
0.01 %
PAR-RAPID
98
0.35 %
578
0.33 %
PAR-RECUR
229
0.82 %
5137
2.92 %
Total(% of all samples)
27357
98.31 %
172473
97.91 %
Serial/Extended Tests
SER-ANOM
7
0.03 %
19
0.01 %
SER-FLEXI
464
1.67 %
3613
2.05 %
SER-FRAUD
0
0.00 %
SER-NSR
0
0.00 %
4
0.00 %
SER-OTHER
0
0.00 %
52
0.03 %
Total(% of all samples)
471
1.69 %
3688
2.09 %
Total
27828
100.00 %
176161
100.00 %
Retests
Total (% of all samples)
1986
7.14 %
11974
6.80 %
England
1593
5.72 %
9479
5.38 %
Wales
381
1.37 %
2416
1.37 %
Scotland
12
0.04 %
79
0.04 %
Resamples
Total (% of all samples)
1511
5.43 %
10098
5.73 %
England
1200
4.31 %
8074
4.58 %
Wales
302
1.09 %
1955
1.11 %
Scotland
9
0.03 %
69
0.04 %
Rejects
Total (% of all samples)
19
0.07 %
548
0.31 %
England
17
0.06 %
435
0.25 %
Wales
2
0.01 %
103
0.06 %
Scotland
0
0.00 %
10
0.01 %
Table 1. Monthly Totals for June 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-IR-EX
PAR-IR-SEV
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-POSTM
PAR-RAPID
PAR-RECUR
SER-ANOM
SER-FLEXI
Breakdown of
Reason for
submission
PAR-CUL-N
14062
50.5 %
PAR-CUL-P
927
3.3 %
PAR-HERD-S
129
0.5 %
PAR-IR-EX
85
0.3 %
PAR-IR-SEV
177
0.6 %
PAR-NEW-IN
7282
26.2 %
PAR-OTHER
75
0.3 %
PAR-PERSIS
4283
15.4 %
PAR-POSTM
10
0.0 %
PAR-RAPID
98
0.4 %
PAR-RECUR
229
0.8 %
SER-ANOM
7
0.0 %
SER-FLEXI
464
1.7 %
Total
27828
100.00 %
Figure 1. Number of samples submitted in each category during June 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with
concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial
other

0
1000
2000
3000
4000
5000
6000
Ayrshire
Argyll & Bute
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Staffs
Somerset excl N
Oxon
N Yorks
Notts
Northants
Leics & Rut
Heref
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown of
Samples
submitted by
County
Figure 2. Number of samples submitted in each category by County during June 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):
parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel
other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial
potential fraud, SER-OTHER: serial other

0
5000
10000
15000
20000
25000
30000
35000
Lanarkshire
Dumfries & G
Ayrshire
Argyll & Bute
W Glamorgan
S Wales
Shropshire
S Glamorgan
Powys
Pembs
NE Wales
Gwynedd
Gwent
Ceredigion
Carms
Worcs
Wiltshire
Warks
S Yorks
Suffolk
Staffs
Somerset excl N
Shropshire
Oxon
N Yorks
Notts
Northants
Lincs
Leics & Rut
Lancashire
Herts
Heref
Hampshire
Gr Manchester
Gloucs
E Sussex
E Riding N Lincs
Dorset
Devon
Derbyshire
Cumbria
Corn & Scilly
Cleveland
Cheshire
Bucks
Berks
Avon
Scotland
Wales
England
SER-OTHER
SER-NSR
SER-FLEXI
SER-ANOM
PAR-RECUR
PAR-RAPID
PAR-POSTM
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-IR-SEV
PAR-IR-EX
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown
of Samples
submitted
by County
Figure 3. Number of samples submitted in each category by County during 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN
(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,
PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent
Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-P
1
5
0.02 %
0
0.00 %
5
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-ANOM
1
7
0.03 %
0
0.00 %
7
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Corn & Scilly
PAR-CUL-N
13
505
1.81 %
15
2.97 %
448
88.71 %
56
11.09 %
42
8.32 %
0
0.00 %
PAR-CUL-P
3
74
0.27 %
3
4.05 %
58
78.38 %
15
20.27 %
13
17.57 %
0
0.00 %
Devon
PAR-CUL-N
43
5,258
18.89 %
254
4.83 %
4741
90.17 %
343
6.52 %
260
4.94 %
3
0.06 %
PAR-CUL-P
2
15
0.05 %
2
13.33 %
11
73.33 %
2
13.33 %
2
13.33 %
0
0.00 %
PAR-NEW-IN
1
9
0.03 %
1
11.11 %
8
88.89 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-PERSIS
1
196
0.70 %
8
4.08 %
178
90.82 %
13
6.63 %
10
5.10 %
0
0.00 %
Dorset
PAR-CUL-N
13
1,928
6.93 %
153
7.94 %
1713
88.85 %
82
4.25 %
62
3.22 %
0
0.00 %
PAR-CUL-P
1
64
0.23 %
7
10.94 %
52
81.25 %
5
7.81 %
5
7.81 %
0
0.00 %
PAR-PERSIS
2
405
1.46 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Gloucs
PAR-CUL-N
6
75
0.27 %
2
2.67 %
61
81.33 %
13
17.33 %
12
16.00 %
0
0.00 %
PAR-PERSIS
2
563
2.02 %
11
1.95 %
499
88.63 %
86
15.28 %
52
9.24 %
1
0.18 %
Heref
PAR-CUL-N
1
51
0.18 %
0
0.00 %
41
80.39 %
12
23.53 %
10
19.61 %
0
0.00 %
PAR-OTHER
1
57
0.20 %
1
1.75 %
51
89.47 %
6
10.53 %
5
8.77 %
0
0.00 %
Somerset excl N
PAR-CUL-N
13
2,529
9.09 %
147
5.81 %
2308
91.26 %
101
3.99 %
74
2.93 %
0
0.00 %
PAR-CUL-P
5
769
2.76 %
27
3.51 %
706
91.81 %
38
4.94 %
34
4.42 %
2
0.26 %
PAR-PERSIS
3
652
2.34 %
21
3.22 %
608
93.25 %
29
4.45 %
22
3.37 %
1
0.15 %
Staffs
PAR-CUL-N
12
1,269
4.56 %
52
4.10 %
1175
92.59 %
65
5.12 %
42
3.31 %
0
0.00 %
Wiltshire
PAR-CUL-N
5
470
1.69 %
25
5.32 %
414
88.09 %
41
8.72 %
31
6.60 %
0
0.00 %
PAR-PERSIS
4
1,038
3.73 %
36
3.47 %
943
90.85 %
74
7.13 %
59
5.68 %
0
0.00 %
England - Edge
Berks
PAR-NEW-IN
2
45
0.16 %
3
6.67 %
32
71.11 %
10
22.22 %
10
22.22 %
0
0.00 %
Bucks
PAR-PERSIS
2
27
0.10 %
2
7.41 %
23
85.19 %
2
7.41 %
2
7.41 %
0
0.00 %
Cheshire
PAR-CUL-N
10
1,977
7.10 %
107
5.41 %
1798
90.95 %
134
6.78 %
72
3.64 %
0
0.00 %
PAR-NEW-IN
2
311
1.12 %
5
1.61 %
272
87.46 %
39
12.54 %
27
8.68 %
7
2.25 %
Derbyshire
PAR-NEW-IN
19
1,412
5.07 %
63
4.46 %
1229
87.04 %
138
9.77 %
120
8.50 %
0
0.00 %
Table 2a. Summary by County for June 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for June 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
E Sussex
PAR-NEW-IN
2
41
0.15 %
0
0.00 %
38
92.68 %
2
4.88 %
2
4.88 %
1
2.44 %
Leics & Rut
PAR-NEW-IN
5
248
0.89 %
9
3.63 %
215
86.69 %
30
12.10 %
24
9.68 %
0
0.00 %
Northants
PAR-NEW-IN
6
451
1.62 %
8
1.77 %
424
94.01 %
27
5.99 %
19
4.21 %
0
0.00 %
Notts
PAR-NEW-IN
2
404
1.45 %
24
5.94 %
358
88.61 %
33
8.17 %
21
5.20 %
1
0.25 %
Oxon
PAR-NEW-IN
5
201
0.72 %
12
5.97 %
168
83.58 %
21
10.45 %
21
10.45 %
0
0.00 %
PAR-PERSIS
3
206
0.74 %
27
13.11 %
163
79.13 %
27
13.11 %
16
7.77 %
0
0.00 %
Warks
PAR-NEW-IN
7
732
2.63 %
20
2.73 %
667
91.12 %
57
7.79 %
45
6.15 %
0
0.00 %
PAR-PERSIS
1
134
0.48 %
7
5.22 %
123
91.79 %
5
3.73 %
4
2.99 %
0
0.00 %
England - LRA
N Yorks
PAR-NEW-IN
3
97
0.35 %
0
0.00 %
29
29.90 %
69
71.13 %
67
69.07 %
1
1.03 %
England
202
22225
79.87 %
1060
4.77 %
19948
89.75 %
1593
7.17 %
1200
5.40 %
17
0.08 %
Wales - High TB
Carms
PAR-IR-EX
2
25
0.09 %
11
44.00 %
14
56.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
7
29
0.10 %
7
24.14 %
20
68.97 %
2
6.90 %
2
6.90 %
0
0.00 %
PAR-PERSIS
1
1
0.00 %
0
0.00 %
1
100.00 %
1
100.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
50
0.18 %
9
18.00 %
39
78.00 %
2
4.00 %
2
4.00 %
0
0.00 %
Ceredigion
PAR-IR-EX
1
8
0.03 %
2
25.00 %
6
75.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
2
3
0.01 %
2
66.67 %
0
0.00 %
1
33.33 %
1
33.33 %
0
0.00 %
PAR-PERSIS
2
85
0.31 %
3
3.53 %
79
92.94 %
7
8.24 %
3
3.53 %
0
0.00 %
PAR-RAPID
4
10
0.04 %
3
30.00 %
6
60.00 %
2
20.00 %
1
10.00 %
0
0.00 %
Gwent
PAR-IR-EX
1
15
0.05 %
1
6.67 %
14
93.33 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
6
0.02 %
1
16.67 %
5
83.33 %
0
0.00 %
0
0.00 %
0
0.00 %
Pembs
PAR-HERD-S
2
10
0.04 %
1
10.00 %
8
80.00 %
2
20.00 %
1
10.00 %
0
0.00 %
PAR-IR-EX
7
37
0.13 %
5
13.51 %
30
81.08 %
3
8.11 %
2
5.41 %
0
0.00 %
PAR-IR-SEV
16
133
0.48 %
34
25.56 %
98
73.68 %
2
1.50 %
1
0.75 %
0
0.00 %
PAR-PERSIS
5
214
0.77 %
15
7.01 %
188
87.85 %
15
7.01 %
10
4.67 %
1
0.47 %
PAR-RAPID
10
14
0.05 %
6
42.86 %
7
50.00 %
1
7.14 %
1
7.14 %
0
0.00 %
PAR-RECUR
4
72
0.26 %
6
8.33 %
64
88.89 %
2
2.78 %
2
2.78 %
0
0.00 %
SER-FLEXI
5
211
0.76 %
17
8.06 %
189
89.57 %
6
2.84 %
5
2.37 %
0
0.00 %
Powys
PAR-PERSIS
6
683
2.45 %
25
3.66 %
585
85.65 %
82
12.01 %
73
10.69 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2a. Summary by County for June 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RAPID
3
3
0.01 %
2
66.67 %
1
33.33 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
73
0.26 %
2
2.74 %
68
93.15 %
5
6.85 %
3
4.11 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-OTHER
3
18
0.06 %
1
5.56 %
16
88.89 %
2
11.11 %
1
5.56 %
0
0.00 %
PAR-RAPID
2
2
0.01 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-IR-SEV
4
12
0.04 %
1
8.33 %
11
91.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
24
2,248
8.08 %
41
1.82 %
2083
92.66 %
160
7.12 %
123
5.47 %
1
0.04 %
PAR-PERSIS
1
79
0.28 %
3
3.80 %
71
89.87 %
5
6.33 %
5
6.33 %
0
0.00 %
PAR-POSTM
3
10
0.04 %
0
0.00 %
10
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
4
11
0.04 %
1
9.09 %
10
90.91 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
1
84
0.30 %
2
2.38 %
77
91.67 %
6
7.14 %
5
5.95 %
0
0.00 %
SER-FLEXI
4
253
0.91 %
9
3.56 %
240
94.86 %
6
2.37 %
4
1.58 %
0
0.00 %
Powys
PAR-NEW-IN
3
275
0.99 %
3
1.09 %
244
88.73 %
33
12.00 %
28
10.18 %
0
0.00 %
Wales - Low TB
Gwynedd
PAR-HERD-S
2
56
0.20 %
1
1.79 %
54
96.43 %
1
1.79 %
1
1.79 %
0
0.00 %
PAR-NEW-IN
8
592
2.13 %
16
2.70 %
553
93.41 %
27
4.56 %
23
3.89 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
NE Wales
PAR-HERD-S
2
63
0.23 %
7
11.11 %
55
87.30 %
3
4.76 %
1
1.59 %
0
0.00 %
PAR-NEW-IN
2
25
0.09 %
0
0.00 %
21
84.00 %
4
16.00 %
4
16.00 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Wales
149
5412
19.45 %
239
4.42 %
4869
89.97 %
381
7.04 %
302
5.58 %
2
0.04 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.68 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
Ayrshire
PAR-NEW-IN
1
3
0.01 %
0
0.00 %
3
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
Scotland
4
191
0.69 %
15
7.85 %
167
87.43 %
12
6.28 %
9
4.71 %
0
0.00 %
Grand Total
Sum:
355
27828
1314
24984
1986
1511
19
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid
testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
England - HRA
Avon
PAR-CUL-N
5
867
0.49 %
66
7.61 %
766
88.35 %
39
4.50 %
32
3.69 %
3
0.35 %
PAR-CUL-P
4
528
0.30 %
31
5.87 %
462
87.50 %
42
7.95 %
35
6.63 %
0
0.00 %
PAR-PERSIS
8
782
0.44 %
132
xx-xxxx-xxxx %
565
72.25 %
85
10.87 %
76
9.72 %
9
1.15 %
SER-ANOM
2
19
0.01 %
0
0.00 %
16
84.21 %
2
10.53 %
2
10.53 %
1
5.26 %
Corn & Scilly
PAR-CUL-N
78
7,003
3.98 %
308
4.40 %
6070
86.68 %
698
9.97 %
612
8.74 %
13
0.19 %
PAR-CUL-P
14
2,558
1.45 %
186
7.27 %
2209
86.36 %
174
6.80 %
161
6.29 %
2
0.08 %
PAR-PERSIS
3
590
0.33 %
30
5.08 %
532
90.17 %
31
5.25 %
28
4.75 %
0
0.00 %
SER-FLEXI
2
264
0.15 %
10
3.79 %
250
94.70 %
6
2.27 %
4
1.52 %
0
0.00 %
Devon
PAR-CUL-N
252
28,490
16.17 %
1883
6.61 %
25093
88.08 %
1675
5.88 %
1411
4.95 %
103
0.36 %
PAR-CUL-P
10
1,099
0.62 %
66
6.01 %
957
87.08 %
92
8.37 %
75
6.82 %
1
0.09 %
PAR-NEW-IN
11
1,002
0.57 %
63
6.29 %
842
84.03 %
97
9.68 %
94
9.38 %
3
0.30 %
PAR-OTHER
3
58
0.03 %
4
6.90 %
49
84.48 %
6
10.34 %
5
8.62 %
0
0.00 %
PAR-PERSIS
3
619
0.35 %
30
4.85 %
556
89.82 %
38
6.14 %
33
5.33 %
0
0.00 %
Dorset
PAR-CUL-N
62
6,610
3.75 %
464
7.02 %
5858
88.62 %
353
5.34 %
283
4.28 %
5
0.08 %
PAR-CUL-P
1
64
0.04 %
7
10.94 %
52
81.25 %
5
7.81 %
5
7.81 %
0
0.00 %
PAR-PERSIS
2
405
0.23 %
8
1.98 %
382
94.32 %
18
4.44 %
15
3.70 %
0
0.00 %
Gloucs
PAR-CUL-N
61
5,592
3.17 %
294
5.26 %
4852
86.77 %
475
8.49 %
412
7.37 %
34
0.61 %
PAR-CUL-P
2
189
0.11 %
35
18.52 %
148
78.31 %
8
4.23 %
6
3.17 %
0
0.00 %
PAR-PERSIS
2
563
0.32 %
11
1.95 %
499
88.63 %
86
15.28 %
52
9.24 %
1
0.18 %
Heref
PAR-CUL-N
10
647
0.37 %
44
6.80 %
528
81.61 %
60
9.27 %
56
8.66 %
19
2.94 %
PAR-OTHER
3
620
0.35 %
20
3.23 %
534
86.13 %
69
11.13 %
66
10.65 %
0
0.00 %
PAR-PERSIS
2
110
0.06 %
7
6.36 %
87
79.09 %
16
14.55 %
16
14.55 %
0
0.00 %
SER-OTHER
1
52
0.03 %
0
0.00 %
51
98.08 %
1
1.92 %
1
1.92 %
0
0.00 %
Shropshire
PAR-CUL-N
24
4,148
2.35 %
172
4.15 %
3751
90.43 %
241
5.81 %
214
5.16 %
11
0.27 %
PAR-OTHER
5
523
0.30 %
20
3.82 %
488
93.31 %
17
3.25 %
7
1.34 %
8
1.53 %
Somerset excl N
PAR-CUL-N
67
7,206
4.09 %
535
7.42 %
6407
88.91 %
338
4.69 %
254
3.52 %
10
0.14 %
Table 2b. Summary by County for 2021
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-CUL-P
5
769
0.44 %
27
3.51 %
706
91.81 %
38
4.94 %
34
4.42 %
2
0.26 %
PAR-NEW-IN
2
370
0.21 %
40
10.81 %
317
85.68 %
13
3.51 %
13
3.51 %
0
0.00 %
PAR-OTHER
2
467
0.27 %
22
4.71 %
389
83.30 %
35
7.49 %
56
11.99 %
0
0.00 %
PAR-PERSIS
4
949
0.54 %
34
3.58 %
878
92.52 %
50
5.27 %
35
3.69 %
2
0.21 %
Staffs
PAR-CUL-N
62
8,943
5.08 %
358
4.00 %
8032
89.81 %
627
7.01 %
551
6.16 %
2
0.02 %
PAR-CUL-P
3
26
0.01 %
2
7.69 %
18
69.23 %
6
23.08 %
6
23.08 %
0
0.00 %
PAR-NEW-IN
3
44
0.02 %
2
4.55 %
36
81.82 %
7
15.91 %
6
13.64 %
0
0.00 %
Wiltshire
PAR-CUL-N
28
2,675
1.52 %
209
7.81 %
2285
85.42 %
207
7.74 %
174
6.50 %
7
0.26 %
PAR-CUL-P
6
450
0.26 %
19
4.22 %
395
87.78 %
26
5.78 %
18
4.00 %
18
4.00 %
PAR-NEW-IN
2
191
0.11 %
26
13.61 %
152
79.58 %
15
7.85 %
13
6.81 %
0
0.00 %
PAR-PERSIS
9
1,677
0.95 %
76
4.53 %
1516
90.40 %
98
5.84 %
78
4.65 %
7
0.42 %
SER-FLEXI
2
172
0.10 %
13
7.56 %
148
86.05 %
1
0.58 %
1
0.58 %
10
5.81 %
Worcs
PAR-CUL-N
2
128
0.07 %
12
9.38 %
112
87.50 %
3
2.34 %
2
1.56 %
2
1.56 %
England - Edge
Berks
PAR-CUL-P
2
319
0.18 %
3
0.94 %
292
91.54 %
10
3.13 %
6
1.88 %
18
5.64 %
PAR-NEW-IN
14
992
0.56 %
61
6.15 %
843
84.98 %
96
9.68 %
88
8.87 %
0
0.00 %
PAR-PERSIS
3
119
0.07 %
8
6.72 %
108
90.76 %
5
4.20 %
3
2.52 %
0
0.00 %
Bucks
PAR-CUL-N
1
17
0.01 %
1
5.88 %
15
88.24 %
1
5.88 %
1
5.88 %
0
0.00 %
PAR-NEW-IN
9
1,360
0.77 %
30
2.21 %
1283
94.34 %
60
4.41 %
46
3.38 %
1
0.07 %
PAR-PERSIS
4
379
0.22 %
7
1.85 %
353
93.14 %
22
5.80 %
19
5.01 %
0
0.00 %
Cheshire
PAR-CUL-N
51
7,280
4.13 %
241
3.31 %
6739
92.57 %
401
5.51 %
299
4.11 %
1
0.01 %
PAR-NEW-IN
27
3,917
2.22 %
118
3.01 %
3569
91.12 %
273
6.97 %
222
5.67 %
8
0.20 %
PAR-PERSIS
3
287
0.16 %
6
2.09 %
268
93.38 %
15
5.23 %
12
4.18 %
1
0.35 %
Derbyshire
PAR-CUL-N
7
985
0.56 %
54
5.48 %
816
82.84 %
119
12.08 %
115
11.68 %
0
0.00 %
PAR-NEW-IN
95
9,508
5.40 %
415
4.36 %
8182
86.05 %
974
10.24 %
844
8.88 %
67
0.70 %
E Sussex
PAR-NEW-IN
10
806
0.46 %
82
10.17 %
660
81.89 %
54
6.70 %
47
5.83 %
17
2.11 %
Hampshire
PAR-CUL-N
3
220
0.12 %
14
6.36 %
201
91.36 %
6
2.73 %
5
2.27 %
0
0.00 %
PAR-NEW-IN
12
1,892
1.07 %
62
3.28 %
xx-xxxx-xxxx
89.43 %
150
7.93 %
136
7.19 %
2
0.11 %
PAR-PERSIS
1
76
0.04 %
3
3.95 %
69
90.79 %
6
7.89 %
4
5.26 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Leics & Rut
PAR-NEW-IN
27
2,720
1.54 %
127
4.67 %
2426
89.19 %
194
7.13 %
167
6.14 %
0
0.00 %
PAR-PERSIS
3
910
0.52 %
24
2.64 %
836
91.87 %
55
6.04 %
49
5.38 %
1
0.11 %
Northants
PAR-NEW-IN
31
2,121
1.20 %
61
2.88 %
1944
91.65 %
147
6.93 %
116
5.47 %
0
0.00 %
PAR-PERSIS
3
184
0.10 %
4
2.17 %
168
91.30 %
14
7.61 %
12
6.52 %
0
0.00 %
Notts
PAR-NEW-IN
3
416
0.24 %
24
5.77 %
367
88.22 %
36
8.65 %
24
5.77 %
1
0.24 %
Oxon
PAR-NEW-IN
45
3,479
1.97 %
125
3.59 %
3146
90.43 %
227
6.52 %
204
5.86 %
4
0.11 %
PAR-PERSIS
8
997
0.57 %
55
5.52 %
896
89.87 %
62
6.22 %
46
4.61 %
0
0.00 %
Warks
PAR-NEW-IN
48
3,815
2.17 %
144
3.77 %
3437
90.09 %
258
6.76 %
224
5.87 %
10
0.26 %
PAR-PERSIS
3
163
0.09 %
10
6.13 %
141
86.50 %
13
7.98 %
12
7.36 %
0
0.00 %
England - LRA
Cleveland
PAR-NEW-IN
2
194
0.11 %
5
2.58 %
178
91.75 %
13
6.70 %
11
5.67 %
0
0.00 %
Cumbria
PAR-CUL-N
1
18
0.01 %
0
0.00 %
6
33.33 %
10
55.56 %
10
55.56 %
2
11.11 %
PAR-NEW-IN
13
2,162
1.23 %
68
3.15 %
1980
91.58 %
125
5.78 %
104
4.81 %
10
0.46 %
E Riding N Lincs
PAR-NEW-IN
1
94
0.05 %
0
0.00 %
91
96.81 %
3
3.19 %
3
3.19 %
0
0.00 %
Gr Manchester
PAR-NEW-IN
6
540
0.31 %
18
3.33 %
503
93.15 %
24
4.44 %
19
3.52 %
0
0.00 %
Herts
PAR-NEW-IN
4
107
0.06 %
9
8.41 %
95
88.79 %
1
0.93 %
1
0.93 %
2
1.87 %
Lancashire
PAR-NEW-IN
7
605
0.34 %
18
2.98 %
549
90.74 %
35
5.79 %
34
5.62 %
4
0.66 %
Lincs
PAR-NEW-IN
12
1,134
0.64 %
41
3.62 %
1015
89.51 %
81
7.14 %
72
6.35 %
6
0.53 %
N Yorks
PAR-NEW-IN
13
517
0.29 %
27
5.22 %
382
73.89 %
114
22.05 %
106
20.50 %
2
0.39 %
Suffolk
PAR-NEW-IN
4
328
0.19 %
13
3.96 %
273
83.23 %
43
13.11 %
39
11.89 %
3
0.91 %
S Yorks
PAR-NEW-IN
3
206
0.12 %
11
5.34 %
161
78.16 %
34
16.50 %
32
15.53 %
2
0.97 %
England
1246
136336
77.39 %
7155
5.25 %
120672
88.51 %
9479
6.95 %
8074
5.92 %
435
0.32 %
Wales - High TB
Carms
PAR-IR-EX
8
47
0.03 %
20
42.55 %
24
51.06 %
2
4.26 %
2
4.26 %
1
2.13 %
PAR-IR-SEV
20
123
0.07 %
31
25.20 %
85
69.11 %
8
6.50 %
7
5.69 %
0
0.00 %
PAR-NEW-IN
1
6
0.00 %
2
33.33 %
4
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-OTHER
15
357
0.20 %
46
12.89 %
290
81.23 %
23
6.44 %
19
5.32 %
2
0.56 %
PAR-PERSIS
18
881
0.50 %
54
6.13 %
791
89.78 %
48
5.45 %
36
4.09 %
0
0.00 %
PAR-RAPID
40
119
0.07 %
31
26.05 %
85
71.43 %
3
2.52 %
3
2.52 %
0
0.00 %
PAR-RECUR
5
245
0.14 %
9
3.67 %
225
91.84 %
13
5.31 %
10
4.08 %
1
0.41 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
SER-FLEXI
4
89
0.05 %
20
22.47 %
68
76.40 %
4
4.49 %
1
1.12 %
0
0.00 %
Ceredigion
PAR-IR-EX
4
15
0.01 %
2
13.33 %
13
86.67 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
22
134
0.08 %
38
28.36 %
89
66.42 %
9
6.72 %
6
4.48 %
1
0.75 %
PAR-OTHER
2
39
0.02 %
3
7.69 %
31
79.49 %
6
15.38 %
5
12.82 %
0
0.00 %
PAR-PERSIS
13
1,015
0.58 %
63
6.21 %
894
88.08 %
66
6.50 %
45
4.43 %
13
1.28 %
PAR-RAPID
24
67
0.04 %
17
25.37 %
47
70.15 %
5
7.46 %
3
4.48 %
0
0.00 %
PAR-RECUR
1
5
0.00 %
1
20.00 %
3
60.00 %
1
20.00 %
1
20.00 %
0
0.00 %
SER-FLEXI
4
147
0.08 %
13
8.84 %
129
87.76 %
5
3.40 %
4
2.72 %
1
0.68 %
Gwent
PAR-IR-EX
1
15
0.01 %
1
6.67 %
14
93.33 %
1
6.67 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
4
23
0.01 %
0
0.00 %
22
95.65 %
1
4.35 %
1
4.35 %
0
0.00 %
PAR-OTHER
4
159
0.09 %
9
5.66 %
146
91.82 %
7
4.40 %
4
2.52 %
0
0.00 %
PAR-PERSIS
9
364
0.21 %
24
6.59 %
320
87.91 %
20
5.49 %
19
5.22 %
1
0.27 %
PAR-RAPID
5
18
0.01 %
4
22.22 %
14
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
115
0.07 %
10
8.70 %
100
86.96 %
5
4.35 %
5
4.35 %
0
0.00 %
Pembs
PAR-HERD-S
2
10
0.01 %
1
10.00 %
8
80.00 %
2
20.00 %
1
10.00 %
0
0.00 %
PAR-IR-EX
18
89
0.05 %
15
xx-xxxx-xxxx %
69
77.53 %
6
6.74 %
5
5.62 %
0
0.00 %
PAR-IR-SEV
72
590
0.33 %
149
25.25 %
424
71.86 %
22
3.73 %
17
2.88 %
0
0.00 %
PAR-OTHER
30
1,290
0.73 %
78
6.05 %
1154
89.46 %
66
5.12 %
55
4.26 %
3
0.23 %
PAR-PERSIS
78
4,691
2.66 %
185
3.94 %
4328
92.26 %
232
4.95 %
170
3.62 %
8
0.17 %
PAR-RAPID
92
268
0.15 %
82
30.60 %
178
66.42 %
13
4.85 %
8
2.99 %
0
0.00 %
PAR-RECUR
23
3,376
1.92 %
151
4.47 %
3118
92.36 %
122
3.61 %
96
2.84 %
11
0.33 %
SER-FLEXI
42
1,490
0.85 %
136
9.13 %
1310
87.92 %
54
3.62 %
37
2.48 %
7
0.47 %
Powys
PAR-IR-EX
8
57
0.03 %
17
29.82 %
36
63.16 %
5
8.77 %
4
7.02 %
0
0.00 %
PAR-IR-SEV
11
75
0.04 %
22
29.33 %
52
69.33 %
3
4.00 %
1
1.33 %
0
0.00 %
PAR-NEW-IN
1
239
0.14 %
4
1.67 %
214
89.54 %
20
8.37 %
20
8.37 %
1
0.42 %
PAR-OTHER
16
804
0.46 %
41
5.10 %
714
88.81 %
58
7.21 %
43
5.35 %
6
0.75 %
PAR-PERSIS
33
1,622
0.92 %
55
3.39 %
1401
86.37 %
173
10.67 %
158
9.74 %
8
0.49 %
PAR-RAPID
16
32
0.02 %
5
15.63 %
27
84.38 %
1
3.13 %
0
0.00 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
PAR-RECUR
3
525
0.30 %
22
4.19 %
477
90.86 %
27
5.14 %
24
4.57 %
2
0.38 %
SER-FLEXI
6
115
0.07 %
17
14.78 %
89
77.39 %
12
10.43 %
9
7.83 %
0
0.00 %
S Wales
PAR-HERD-S
5
1,030
0.58 %
44
4.27 %
944
91.65 %
43
4.17 %
37
3.59 %
5
0.49 %
PAR-IR-SEV
1
18
0.01 %
4
22.22 %
14
77.78 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
1
2
0.00 %
0
0.00 %
2
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
W Glamorgan
PAR-OTHER
2
67
0.04 %
0
0.00 %
61
91.04 %
7
10.45 %
6
8.96 %
0
0.00 %
PAR-PERSIS
2
136
0.08 %
2
1.47 %
129
94.85 %
5
3.68 %
5
3.68 %
0
0.00 %
Wales - Intermediate TB
Carms
PAR-HERD-S
2
9
0.01 %
1
11.11 %
7
77.78 %
1
11.11 %
1
11.11 %
0
0.00 %
PAR-OTHER
8
678
0.38 %
66
9.73 %
582
85.84 %
38
5.60 %
27
3.98 %
3
0.44 %
PAR-PERSIS
6
435
0.25 %
35
8.05 %
379
87.13 %
27
6.21 %
21
4.83 %
0
0.00 %
PAR-RAPID
5
7
0.00 %
3
42.86 %
4
57.14 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
3
219
0.12 %
20
9.13 %
187
85.39 %
13
5.94 %
11
5.02 %
1
0.46 %
SER-FLEXI
4
85
0.05 %
22
25.88 %
60
70.59 %
6
7.06 %
3
3.53 %
0
0.00 %
Ceredigion
PAR-OTHER
3
106
0.06 %
6
5.66 %
93
87.74 %
10
9.43 %
6
5.66 %
1
0.94 %
PAR-PERSIS
2
73
0.04 %
5
6.85 %
67
91.78 %
3
4.11 %
1
1.37 %
0
0.00 %
PAR-RAPID
1
4
0.00 %
2
50.00 %
2
50.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
139
0.08 %
8
5.76 %
125
89.93 %
9
6.47 %
6
4.32 %
0
0.00 %
SER-FLEXI
3
148
0.08 %
6
4.05 %
139
93.92 %
3
2.03 %
2
1.35 %
1
0.68 %
Gwynedd
PAR-NEW-IN
3
467
0.27 %
15
3.21 %
407
87.15 %
48
10.28 %
44
9.42 %
1
0.21 %
NE Wales
PAR-IR-EX
1
4
0.00 %
3
75.00 %
1
25.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-IR-SEV
20
128
0.07 %
21
xx-xxxx-xxxx %
99
77.34 %
10
7.81 %
8
6.25 %
0
0.00 %
PAR-NEW-IN
82
7,027
3.99 %
248
3.53 %
6382
90.82 %
469
6.67 %
389
5.54 %
8
0.11 %
PAR-OTHER
5
106
0.06 %
4
3.77 %
95
89.62 %
10
9.43 %
7
6.60 %
0
0.00 %
PAR-PERSIS
11
1,092
0.62 %
39
3.57 %
995
91.12 %
75
6.87 %
58
5.31 %
0
0.00 %
PAR-POSTM
3
10
0.01 %
0
0.00 %
10
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RAPID
12
49
0.03 %
11
22.45 %
37
75.51 %
1
2.04 %
1
2.04 %
0
0.00 %
PAR-RECUR
3
200
0.11 %
21
10.50 %
169
84.50 %
11
5.50 %
10
5.00 %
0
0.00 %
SER-FLEXI
12
477
0.27 %
33
6.92 %
432
90.57 %
21
4.40 %
12
2.52 %
0
0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 2b. Summary by County for 2021 Cont.
Country
County
Submission
Reasons*
No.
subs
Samples
Gamma Positive
Gamma Negatives
Retest*
Resample
Reject
n
% of total
n
%
n
%
n
%
Powys
PAR-IR-EX
1
4
0.00 %
0
0.00 %
4
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-NEW-IN
17
1,762
1.00 %
53
3.01 %
1565
88.82 %
158
8.97 %
141
8.00 %
3
0.17 %
PAR-PERSIS
1
14
0.01 %
2
14.29 %
8
57.14 %
4
28.57 %
4
28.57 %
0
0.00 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
92
0.05 %
6
6.52 %
81
88.04 %
7
7.61 %
4
4.35 %
1
1.09 %
S Glamorgan
PAR-RAPID
2
7
0.00 %
5
71.43 %
2
28.57 %
0
0.00 %
0
0.00 %
0
0.00 %
PAR-RECUR
2
221
0.13 %
21
9.50 %
191
86.43 %
10
4.52 %
9
4.07 %
0
0.00 %
Shropshire
PAR-NEW-IN
3
126
0.07 %
7
5.56 %
98
77.78 %
18
14.29 %
17
13.49 %
4
3.17 %
Wales - Low TB
Gwynedd
PAR-HERD-S
3
57
0.03 %
1
1.75 %
55
96.49 %
1
1.75 %
1
1.75 %
0
0.00 %
PAR-NEW-IN
23
1,119
0.64 %
38
3.40 %
1010
90.26 %
85
7.60 %
68
6.08 %
3
0.27 %
PAR-RAPID
3
3
0.00 %
1
33.33 %
2
66.67 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
2
19
0.01 %
5
26.32 %
13
68.42 %
1
5.26 %
1
5.26 %
0
0.00 %
NE Wales
PAR-HERD-S
4
111
0.06 %
9
8.11 %
99
89.19 %
5
4.50 %
3
2.70 %
0
0.00 %
PAR-NEW-IN
31
2,456
1.39 %
122
4.97 %
2141
87.17 %
223
9.08 %
189
7.70 %
4
0.16 %
PAR-OTHER
4
152
0.09 %
4
2.63 %
140
92.11 %
8
5.26 %
7
4.61 %
1
0.66 %
PAR-PERSIS
2
43
0.02 %
2
4.65 %
40
93.02 %
0
0.00 %
0
0.00 %
1
2.33 %
PAR-RAPID
1
1
0.00 %
0
0.00 %
1
100.00 %
0
0.00 %
0
0.00 %
0
0.00 %
SER-FLEXI
5
607
0.34 %
49
8.07 %
521
85.83 %
43
7.08 %
37
6.10 %
0
0.00 %
Wales
966
38767
22.01 %
2322
5.99 %
34387
88.70 %
2416
6.23 %
1955
5.04 %
103
0.27 %
Scotland - Scotland
Argyll & Bute
PAR-NEW-IN
3
188
0.11 %
15
7.98 %
164
87.23 %
12
6.38 %
9
4.79 %
0
0.00 %
SER-NSR
1
4
0.00 %
0
0.00 %
3
75.00 %
0
0.00 %
0
0.00 %
1
25.00 %
Ayrshire
PAR-NEW-IN
7
846
0.48 %
113
13.36 %
666
78.72 %
65
7.68 %
58
6.86 %
9
1.06 %
Dumfries & G
PAR-NEW-IN
1
8
0.00 %
0
0.00 %
7
87.50 %
1
12.50 %
1
12.50 %
0
0.00 %
Lanarkshire
PAR-NEW-IN
1
12
0.01 %
1
8.33 %
10
83.33 %
1
8.33 %
1
8.33 %
0
0.00 %
Scotland
13
1058
0.60 %
129
12.19 %
850
80.34 %
79
7.47 %
69
6.52 %
10
0.95 %
Grand Total
Sum:
xx-xxxx-xxxx
9606
155909
11974
10098
548
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing
of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s
infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is
possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative
result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Table 3a. Summary of IFN Gamma results by country and protocol June 2021
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
201
22218
100.0%
1060
4.8%
19941
89.8%
1593
7.2%
1200
5.4%
17
0.1%
Serial/Extended
1
7
0.0%
0
0.0 %
7 100.0%
0
0.0 %
0
0.0 %
0
0.0 %
Total
202
22225
100.0%
1060
4.8%
19948
89.8%
1593
7.2%
1200
5.4%
17
0.1%
Scotland
Parallel
4
191
100.0%
15
7.9%
167
87.4%
12
6.3%
9
4.7%
0
0.0 %
Serial/Extended
0
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
0
0.0 %
Total
4
191
100.0%
15
7.9%
167
87.4%
12
6.3%
9
4.7%
0
0.0 %
Wales
Parallel
140
4948
91.4%
213
4.3%
4440
89.7%
369
7.5%
293
5.9%
2
0.0%
Serial/Extended
9
464
8.6%
26
5.6%
429
92.5%
12
2.6%
9
1.9%
0
0.0 %
Total
149
5412
100.0%
239
4.4%
4869
90.0%
381
7.0%
302
5.6%
2
0.0%
GB
Parallel
345
27357
98.3%
1288
4.7%
24548
89.7%
1974
7.2%
1502
5.5%
19
0.1%
Serial/Extended
10
471
1.7%
26
5.5%
436
92.6%
12
2.5%
9
1.9%
0
0.0 %
Total
355
27828
100.0%
1314
4.7%
24984
89.8%
1986
7.1%
1511
5.4%
19
0.1%
202.00
149.00
4.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2021)
Country
Protocol
No
submissions Samples
Positive
Negatives
Retest*
Resample
Reject
n
% of total n
%
n
%
n
%
n
%
n
%
England
Parallel
xx-xxxx-xxxx
99.6%
7132
5.3% 120207
88.5%
9469
7.0%
8066
5.9%
424
0.3%
Serial/Extended
7
507
0.4%
23
4.5%
465
91.7%
10
2.0%
8
1.6%
11
2.2%
Total
xx-xxxx-xxxx
100.0%
7155
5.2% 120672
88.5%
9479
7.0%
8074
5.9%
435
0.3%
Scotland
Parallel
12
1054
99.6%
129
12.2%
847
80.4%
79
7.5%
69
6.5%
9
0.9%
Serial/Extended
1
4
0.4%
0
0.0 %
3
75.0%
0
0.0 %
0
0.0 %
1
25.0%
Total
13
1058
100.0%
129
12.2%
850
80.3%
79
7.5%
69
6.5%
10
0.9%
Wales
Parallel
884
35590
91.8%
2021
5.7%
31626
88.9%
2267
6.4%
1849
5.2%
94
0.3%
Serial/Extended
82
3177
8.2%
301
9.5%
2761
86.9%
149
4.7%
106
3.3%
9
0.3%
Total
966
38767
100.0%
2322
6.0%
34387
88.7%
2416
6.2%
1955
5.0%
103
0.3%
GB
Parallel
xx-xxxx-xxxx
97.9%
9282
5.4% 152680
88.5%
11815
6.9%
9984
5.8%
527
0.3%
Serial/Extended
90
3688
2.1%
324
8.8%
3229
87.6%
159
4.3%
114
3.1%
21
0.6%
Total
xx-xxxx-xxxx
100.0%
9606
5.5% 155909
88.5%
11974
6.8%
10098
5.7%
548
0.3%
#######
966.00
13.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory
to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number
of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Extended Flexible IFN-GAMMA test
9
464
1.7%
26
5.6%
429
92.5%
12
2.6 %
9
1.9 %
0
Parallel - Herd or Group Slaughter
6
129
0.5%
9
7.0%
117
90.7%
6
4.7 %
3
2.3 %
0
Parallel - IRs in Exit Strategy for persistent her
11
85
0.3%
19
22.4%
64
75.3%
4
4.7 %
2
2.4 %
0
Parallel New Infection
95
7282
26.2%
220
3.0%
6508
89.4%
662
9.1 %
543
7.5 %
11
0.2 %
Parallel - new infection cull area
116
14062
50.5%
755
5.4%
12699
90.3%
847
6.0 %
605
4.3 %
3
0.0 %
Parallel - Other
4
75
0.3%
2
2.7%
67
89.3%
8
10.7 %
6
8.0 %
0
Parallel Persistent Infection
33
4283
15.4%
166
3.9%
3843
89.7%
364
8.5 %
271
6.3 %
3
0.1 %
Parallel - persistent infection cull area
12
927
3.3%
39
4.2%
832
89.8%
60
6.5 %
54
5.8 %
2
0.2 %
Parallel - Rapid Testing Two-times IRs
30
98
0.4%
24
24.5%
70
71.4%
5
5.1 %
4
4.1 %
0
Parallel - Recurrent breakdowns
6
229
0.8%
10
4.4%
209
91.3%
13
5.7 %
10
4.4 %
0
Parallel - severe IRs - in persistent herds
29
177
0.6%
44
24.9%
129
72.9%
5
2.8 %
4
2.3 %
0
PAR-POSTM
3
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Total
355
27828
100.0%
1314
4.7%
24984
89.8%
1986
7.1 %
1511
5.4 %
19
0.1 %
Table 4a. Summaryof IFN Gamma results by submission reason (June 2021)
England - HRA
Anomalous reaction
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Parallel New Infection
1
9
0.0%
1
11.1%
8
88.9%
0
0
0
Parallel - new infection cull area
106
12085
43.4%
648
5.4%
10901
90.2%
713
5.9 %
533
4.4 %
3
0.0 %
Parallel - Other
1
57
0.2%
1
1.8%
51
89.5%
6
10.5 %
5
8.8 %
0
Parallel Persistent Infection
12
2854
10.3%
84
2.9%
2610
91.5%
220
7.7 %
158
5.5 %
2
0.1 %
Parallel - persistent infection cull area
12
927
3.3%
39
4.2%
832
89.8%
60
6.5 %
54
5.8 %
2
0.2 %
England - HRA
133
15939
57.3%
773
4.8%
14409
90.4%
999
6.3 %
750
4.7 %
7
0.0 %
England - Edge
Parallel New Infection
50
3845
13.8%
144
3.7%
3403
88.5%
357
9.3 %
289
7.5 %
9
0.2 %
Parallel - new infection cull area
10
1977
7.1%
107
5.4%
1798
90.9%
134
6.8 %
72
3.6 %
0
Parallel Persistent Infection
6
367
1.3%
36
9.8%
309
84.2%
34
9.3 %
22
6.0 %
0
England - Edge
66
6189
22.2%
287
4.6%
5510
89.0%
525
8.5 %
383
6.2 %
9
0.1 %
England - LRA
Parallel New Infection
3
97
0.3%
0
0.0 %
29
29.9%
69
71.1 %
67
69.1 %
1
1.0 %
England - LRA
3
97
0.3%
0
0.0 %
29
29.9%
69
71.1 %
67
69.1 %
1
1.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
5
211
0.8%
17
8.1%
189
89.6%
6
2.8 %
5
2.4 %
0
Parallel - Herd or Group Slaughter
2
10
0.0%
1
10.0%
8
80.0%
2
20.0 %
1
10.0 %
0
Parallel - IRs in Exit Strategy for persistent her
11
85
0.3%
19
22.4%
64
75.3%
4
4.7 %
2
2.4 %
0
Parallel Persistent Infection
14
983
3.5%
43
4.4%
853
86.8%
105
10.7 %
86
8.7 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
22
83
0.3%
21
25.3%
58
69.9%
5
6.0 %
4
4.8 %
0
Parallel - Recurrent breakdowns
5
145
0.5%
8
5.5%
132
91.0%
7
4.8 %
5
3.4 %
0
Parallel - severe IRs - in persistent herds
25
165
0.6%
43
26.1%
118
71.5%
5
3.0 %
4
2.4 %
0
Wales - High TB
84
xx-xxxx-xxxx
6.0%
152
9.0%
1422
84.5%
134
8.0 %
107
6.4 %
1
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
253
0.9%
9
3.6%
240
94.9%
6
2.4 %
4
1.6 %
0
Parallel New Infection
27
2523
9.1%
44
1.7%
2327
92.2%
193
7.6 %
151
6.0 %
1
0.0 %
Parallel - Other
3
18
0.1%
1
5.6%
16
88.9%
2
11.1 %
1
5.6 %
0
Parallel Persistent Infection
1
79
0.3%
3
3.8%
71
89.9%
5
6.3 %
5
6.3 %
0
Parallel - Rapid Testing Two-times IRs
6
13
0.0%
3
23.1%
10
76.9%
0
0
0
Parallel - Recurrent breakdowns
1
84
0.3%
2
2.4%
77
91.7%
6
7.1 %
5
6.0 %
0
Parallel - severe IRs - in persistent herds
4
12
0.0%
1
8.3%
11
91.7%
0
0
0
PAR-POSTM
3
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Wales - Intermediate TB
49
2992
10.8%
63
2.1%
2762
92.3%
212
7.1 %
166
5.5 %
1
0.0 %
Wales - Low TB
Parallel - Herd or Group Slaughter
4
119
0.4%
8
6.7%
109
91.6%
4
3.4 %
2
1.7 %
0
Parallel New Infection
10
617
2.2%
16
2.6%
574
93.0%
31
5.0 %
27
4.4 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Low TB
16
738
2.7%
24
3.3%
685
92.8%
35
4.7 %
29
3.9 %
0
Scotland - Scotland
Parallel New Infection
4
191
0.7%
15
7.9%
167
87.4%
12
6.3 %
9
4.7 %
0
Scotland - Scotland
4
191
0.7%
15
7.9%
167
87.4%
12
6.3 %
9
4.7 %
0

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Anomalous reaction
2
19
0.0%
0
0.0 %
16
84.2%
2
10.5 %
2
10.5 %
1
5.3 %
Extended Flexible IFN-GAMMA test
86
3613
2.1%
324
9.0%
3159
87.4%
156
4.3 %
111
3.1 %
19
0.5 %
Parallel - Herd or Group Slaughter
16
1217
0.7%
56
4.6%
1113
91.5%
52
4.3 %
43
3.5 %
5
0.4 %
Parallel - IRs in Exit Strategy for persistent herds
41
231
0.1%
58
25.1%
161
69.7%
14
6.1 %
11
4.8 %
1
0.4 %
Parallel New Infection
577
52776
30.0%
2208
4.2%
46791
88.7%
4174
7.9 %
3602
6.8 %
175
0.3 %
Parallel - new infection cull area
714
80829
45.9%
4655
5.8%
71531
88.5%
5253
6.5 %
4431
5.5 %
212
0.3 %
Parallel - Other
102
5426
3.1%
323
6.0%
4766
87.8%
360
6.6 %
313
5.8 %
24
0.4 %
Parallel Persistent Infection
236
19176
10.9%
911
4.8%
17206
89.7%
1267
6.6 %
1007
5.3 %
52
0.3 %
Parallel - persistent infection cull area
47
6002
3.4%
376
6.3%
5239
87.3%
401
6.7 %
346
5.8 %
41
0.7 %
Parallel - Rapid Testing Two-times IRs
203
578
0.3%
161
27.9%
402
69.6%
23
4.0 %
15
2.6 %
0
Parallel - Recurrent breakdowns
46
5137
2.9%
269
5.2%
4676
91.0%
218
4.2 %
176
3.4 %
16
0.3 %
Parallel - severe IRs - in persistent herds
150
1091
0.6%
265
24.3%
785
72.0%
53
4.9 %
40
3.7 %
1
0.1 %
PAR-POSTM
3
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %
Total
xx-xxxx-xxxx
100.0%
9606
5.5%
155909
88.5%
11974
6.8 %
10098
5.7 %
548
0.3 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2021)
England - HRA
Anomalous reaction
2
19
0.0%
0
0.0 %
16
84.2%
2
10.5 %
2
10.5 %
1
5.3 %
Extended Flexible IFN-GAMMA test
4
436
0.2%
23
5.3%
398
91.3%
7
1.6 %
5
1.1 %
10
2.3 %
Parallel New Infection
18
1607
0.9%
131
8.2%
1347
83.8%
132
8.2 %
126
7.8 %
3
0.2 %
Parallel - new infection cull area
651
72309
41.0%
4345
6.0%
63754
88.2%
4716
6.5 %
4001
5.5 %
209
0.3 %
Parallel - Other
13
1668
0.9%
66
4.0%
1460
87.5%
127
7.6 %
134
8.0 %
8
0.5 %
Parallel Persistent Infection
33
5695
3.2%
328
5.8%
5015
88.1%
422
7.4 %
333
5.8 %
19
0.3 %
Parallel - persistent infection cull area
45
5683
3.2%
373
6.6%
4947
87.0%
391
6.9 %
340
6.0 %
23
0.4 %
Serial - Other
1
52
0.0%
0
0.0 %
51
98.1%
1
1.9 %
1
1.9 %
0
England - Edge
Parallel New Infection
321
31026
17.6%
1249
4.0%
27549
88.8%
2469
8.0 %
2118
6.8 %
110
0.4 %
Parallel - new infection cull area
62
8502
4.8%
310
3.6%
7771
91.4%
527
6.2 %
420
4.9 %
1
0.0 %
Parallel Persistent Infection
28
3115
1.8%
117
3.8%
2839
91.1%
192
6.2 %
157
5.0 %
2
0.1 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Parallel - persistent infection cull area
2
319
0.2%
3
0.9%
292
91.5%
10
3.1 %
6
1.9 %
18
5.6 %
England - LRA
Parallel New Infection
65
5887
3.3%
210
3.6%
5227
88.8%
473
8.0 %
421
7.2 %
29
0.5 %
Parallel - new infection cull area
1
18
0.0%
0
0.0 %
6
33.3%
10
55.6 %
10
55.6 %
2
11.1 %
England
xx-xxxx-xxxx
77.4%
7155
5.2%
120672
88.5%
9479
7.0 %
8074
5.9 %
435
0.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
56
1841
1.0%
186
10.1%
1596
86.7%
75
4.1 %
51
2.8 %
8
0.4 %
Parallel - Herd or Group Slaughter
7
1040
0.6%
45
4.3%
952
91.5%
45
4.3 %
38
3.7 %
5
0.5 %
Parallel - IRs in Exit Strategy for persistent herd
39
223
0.1%
55
24.7%
156
70.0%
14
6.3 %
11
4.9 %
1
0.4 %
Parallel New Infection
2
245
0.1%
6
2.4%
218
89.0%
20
8.2 %
20
8.2 %
1
0.4 %
Parallel - Other
69
2716
1.5%
177
6.5%
2396
88.2%
167
6.1 %
132
4.9 %
11
0.4 %
Parallel Persistent Infection
153
8709
4.9%
383
4.4%
7863
90.3%
544
6.2 %
433
5.0 %
30
0.3 %
Parallel - Rapid Testing Two-times IRs
178
506
0.3%
139
27.5%
353
69.8%
22
4.3 %
14
2.8 %
0
Parallel - Recurrent breakdowns
34
4266
2.4%
193
4.5%
3923
92.0%
168
3.9 %
136
3.2 %
14
0.3 %
Parallel - severe IRs - in persistent herds
130
963
0.5%
244
25.3%
686
71.2%
43
4.5 %
32
3.3 %
1
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
19
710
0.4%
61
8.6%
631
88.9%
30
4.2 %
17
2.4 %
1
0.1 %
Parallel - Herd or Group Slaughter
2
9
0.0%
1
11.1%
7
77.8%
1
11.1 %
1
11.1 %
0
Parallel - IRs in Exit Strategy for persistent herd
2
8
0.0%
3
37.5%
5
62.5%
0
0
0
Parallel New Infection
105
9382
5.3%
323
3.4%
8452
90.1%
693
7.4 %
591
6.3 %
16
0.2 %
Parallel - Other
16
890
0.5%
76
8.5%
770
86.5%
58
6.5 %
40
4.5 %
4
0.4 %
Parallel Persistent Infection
20
1614
0.9%
81
5.0%
1449
89.8%
109
6.8 %
84
5.2 %
0
Parallel - Rapid Testing Two-times IRs
21
68
0.0%
21
30.9%
46
67.6%
1
1.5 %
1
1.5 %
0
Parallel - Recurrent breakdowns
12
871
0.5%
76
8.7%
753
86.5%
50
5.7 %
40
4.6 %
2
0.2 %
Parallel - severe IRs - in persistent herds
20
128
0.1%
21
16.4%
99
77.3%
10
7.8 %
8
6.3 %
0
PAR-POSTM
3
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
7
626
0.4%
54
8.6%
534
85.3%
44
7.0 %
38
6.1 %
0
Parallel - Herd or Group Slaughter
7
168
0.1%
10
6.0%
154
91.7%
6
3.6 %
4
2.4 %
0
Parallel New Infection
54
3575
2.0%
160
4.5%
3151
88.1%
308
8.6 %
257
7.2 %
7
0.2 %
Parallel - Other
4
152
0.1%
4
2.6%
140
92.1%
8
5.3 %
7
4.6 %
1
0.7 %
Parallel Persistent Infection
2
43
0.0%
2
4.7%
40
93.0%
0
0
1
2.3 %
Parallel - Rapid Testing Two-times IRs
4
4
0.0%
1
25.0%
3
75.0%
0
0
0
Wales
966
38767
22.0%
2322
6.0%
34387
88.7%
2416
6.2 %
1955
5.0 %
103
0.3 %
Scotland - Scotland
Parallel New Infection
12
1054
0.6%
129
12.2%
847
80.4%
79
7.5 %
69
6.5 %
9
0.9 %
Serial - Suspected NSR Herd
1
4
0.0%
0
0.0 %
3
75.0%
0
0
1
25.0 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2021) Cont.
Scotland
13
1058
0.6%
129
12.2%
850
80.3%
79
7.5 %
69
6.5 %
10
0.9 %

Year
Month
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
Wrong
Eartags
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
2020 July
332
22622
180
1153
28
0
0
0
0
204
0
0
8
0
842
0
70
0
1
August
290
24676
173
888
15
0
0
0
0
145
1
0
3
0
644
0
80
0
0
September
320
25394
175
762
4
0
0
0
0
69
2
0
4
0
617
0
65
0
1
October
316
26668
189
1198
9
0
0
0
0
114
6
0
2
0
991
0
75
0
0
November
347
26584
200
1359
29
0
0
0
0
105
3
0
7
1
1124
0
88
0
0
December
324
24146
186
1228
1
33
0
0
0
0
104
1
0
9
0
1005
0
72
2
0
2021 January
375
27940
233
1653
2
53
0
0
0
0
111
3
0
14
0
1371
0
97
1
0
February
352
28759
235
1637
42
0
0
0
0
98
3
0
12
0
1380
12
84
5
0
March
419
36344
256
1867
24
0
0
0
0
77
5
0
8
2
1657
4
89
0
1
April
367
28108
211
1571
36
0
0
0
0
105
0
4
10
2
1337
10
64
1
1
May
357
27182
214
1564
45
74
0
0
0
0
127
0
12
0
0
1182
110
5
1
2
June
321
25262
187
1188
120
511
0
0
0
0
38
0
3
0
0
414
34
0
0
0
Totals (last
12 months)
4120
323685
2439
16068
168
858
0
0
0
0
1297
24
19
77
5
12564
170
789
10
6
Table 5a. Summary of PM and culture results for IFNg positive animals.
Please note: “Wrong Eartags” – this column represents those samples in the laboratory information management system (LIMS) for which there is not a matching ear tag in the Cattle TB
Data dataset (SAM) at the time of monthly report trawling. Most resolve in subsequent monthly reports as the field data is added to SAM.

Country
Herds
sampled
Samples
tested
Herds
with
positives
IFNg+
samples
No PM
No
Cult
No PM
Cult
Pend
No PM
Cult
Neg
No PM
Cult
Mb
No
PM
Cult
Other
VL No
Cult
VL
Cult
Neg
VL
Cult
Pend
VL
Cult
Mb
VL
Cult
Other
NVL
No
Cult
NVL
Cult
Pend
NVL
Cult
Neg
NVL
Cult
Mb
NVL
Cult
Other
England
2246
251632
1465
11916
780
0
0
0
0
1142
13
14
40
2
9633
20
91
0
2
Scotland
33
3236
20
188
2
0
0
0
0
47
2
0
7
0
64
12
50
3
0
Wales
1841
68817
954
3964
76
0
0
0
0
108
9
5
30
3
2867
138
648
7
4
Totals
4120
323685
2439
16068
858
0
0
0
0
1297
24
19
77
5
12564
170
789
10
6
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Figure 4a. Number of herds sampled for IFNg and numbers of herds with any
positive IFNg results.
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
116
227
131
192
115
147
88
96
100
127
95
149
152
180
117
173
145
175
127
189
147
200
138
186
142
233
117
235
163
256
156
211
143
214
147
208
0
50
100
150
200
250
300
350
400
450
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
2019
2020
2021
IFNg Positive herds
IFNg Negative herds
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
29262
1367
31244
1289
17118
764
10415
408
xx-xxxx-xxxx
858
22130
860
21221
1153
23461
888
24351
762
25236
1198
24710
1359
22625
1228
25840
1653
26496
1637
33735
1867
26079
1571
25277
1561
26185
1314
0
5000
10000
15000
20000
25000
30000
35000
40000
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
2019
2020
2021
IFNg positive animals
IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals with
positive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes

0
1
2
3
4
5
6
7
8
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
July
August
September
October
November
December
January
February
March
April
May
June
2019
2020
2021
%Rejected
%Resampled
% NEG Fail
% POS Fail
% of total
submitted
Figure 5. Rates of resamples including the fail rates broken down for positive
(POS) and negative (NEG) controls.

0
20
40
60
80
100
120
140
160
Wiltshire
Warks
Staffs
Somerset excl N
Powys
Pembs
Oxon
N Yorks
Notts
Northants
NE Wales
Leics & Rut
Heref
Gwynedd
Gwent
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Ceredigion
Carms
Bucks
Berks
Ayrshire
Avon
Argyll & Bute
%Retested
%Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during June 2021 (% submission per County)
County
%Resampled %Retested
Argyll & Bute
4.79 %
6.38 %
Avon
Ayrshire
Berks
22.22 %
22.22 %
Bucks
7.41 %
7.41 %
Carms
4.00 %
5.60 %
Ceredigion
4.72 %
9.43 %
Cheshire
4.33 %
7.56 %
Corn & Scilly
9.50 %
12.26 %
Derbyshire
8.50 %
9.77 %
Devon
4.97 %
6.54 %
Dorset
3.42 %
4.38 %
E Sussex
4.88 %
4.88 %
Gloucs
10.03 %
15.52 %
Gwent
4.76 %
Gwynedd
3.70 %
4.31 %
Heref
13.89 %
16.67 %
Leics & Rut
9.68 %
12.10 %
NE Wales
5.10 %
6.60 %
Northants
4.21 %
5.99 %
Notts
5.20 %
8.17 %
N Yorks
69.07 %
71.13 %
Oxon
9.09 %
11.79 %
Pembs
3.18 %
4.49 %
Powys
10.06 %
11.61 %
Somerset excl
3.29 %
4.25 %
Staffs
3.31 %
5.12 %
Warks
5.66 %
7.16 %
Wiltshire
5.97 %
7.63 %

Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
22021
1060
4.8%
22225
1060
4.8%
Wales
5292
239
4.5%
5412
239
4.4%
Scotland
186
15
8.1%
191
15
7.9%
GB
27499
1314
4.8%
27828
1314
4.7%
Table 6a (i): Number of animals and submissions tested in June 2021
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
130438
7152
5.5%
136336
7155
5.2%
Wales
36419
2322
6.4%
38767
2322
6.0%
Scotland
577
129
22.4%
1058
129
12.2%
GB
167425
9603
5.7%
176161
9606
5.5%
Table 6b (i): Number of animals and submissions tested in 2021 Up to end of June
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
17917
612
3.4%
18093
612
3.4%
Wales
4126
223
5.4%
4168
223
5.4%
Scotland
947
25
2.6%
956
25
2.6%
GB
22990
860
3.7%
23217
860
3.7%
Table 6a (ii): Number of animals and submissions tested in June 2020
Table 6b (ii): Number of animals and submissions tested in 2020 Up to end of June
Country
No.
animals
No of IFHg+
animals
% Positive
animals
No. samples
No of IFHg+
samples
% Positive
samples
England
99532
3997
4.0%
104673
3997
3.8%
Wales
24035
1446
6.0%
25583
1447
5.7%
Scotland
3609
102
2.8%
4101
102
2.5%
GB
127175
5545
4.4%
134357
5546
4.1%

* Test type
Num
Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SE
2
206
5
2.43 %
Total
2
206
5
2.43 %
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;
PRV-SP: Private High Specificity test – England
Table 7a Number of private samples June 2021
Table 7b Number of private samples 2021
* Test type
Num
Submissions
Num
Samples
Num
Positives
Private
% Positive
Cheshire
PRV-SP
1
3
0
0
Cornwall and Isles of Scilly
PRV-SE
1
4
3
75.00 %
PRV-SP
1
1
0
0
Devon
PRV-SE
2
64
1
1.56 %
PRV-SP
1
2
0
0
Gloucestershire
PRV-SE
1
1
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SE
1
3
0
0
Somerset excl North
PRV-SE
3
207
5
2.42 %
PRV-SP
1
1
0
0
Wiltshire
PRV-SE
1
10
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
15
299
9
3.01 %
Isle of Man
SER-IOM
4
4
0
0
Isle of Man
SER-IOM
1
1
0
0

Country
County
Test Reason
Num
Submissions
Num Samples
Tested
Num Positive
Samples
% Positive
England
Staffordshire
BOV-P
1
30
1
3.3%
England
1
30
1
3.3%
Wales
Carmarthenshire
BOV-IR-C
3
28
6
21.4%
BOV-IR-EX
1
18
4
22.2%
Ceredigion
BOV-IR-EX
1
8
2
25.0%
BOV-P
1
16
0
0
North East Wales
BOV-IR-C
3
13
3
23.1%
BOV-OTHER
1
48
3
6.3%
BOV-P
1
201
10
5.0%
BOV-POSTM
1
8
1
12.5%
OTHER
1
34
2
5.9%
Pembrokeshire
BOV-IR-C
10
107
26
24.3%
BOV-IR-EX
2
11
2
18.2%
BOV-OTHER
1
12
1
8.3%
BOV-P
2
83
7
8.4%
Wales
28
587
67
11.4%
All GB
29
617
68
11.0 %
Table 8a Number of Cattle Antibody Tests June 2021
Table 8b Number of Cattle Antibody Tests 2021
Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
England
Cornwall and Isles of
Scilly
BOV-P
1
90
0
0
Devon
1
95
6
6.3%
Staffordshire
BOV-P
1
30
1
3.3%
England
3
215
7
3.3%
Wales
Carmarthenshire
4
16
3
18.8%
BOV-IR-C
17
177
36
20.3%
BOV-IR-EX
4
36
9
25.0%
BOV-OTHER
2
26
2
7.7%
BOV-P
1
26
2
7.7%
OTHER
2
72
8
11.1%
Ceredigion
2
16
1
6.3%
BOV-IR-C
19
160
23
14.4%
BOV-IR-EX
1
8
2
25.0%
BOV-OTHER
6
240
16
6.7%
BOV-P
1
16
0
0
North East Wales
2
24
6
25.0%
BOV-IR-C
15
159
23
14.5%
BOV-IR-EX
1
4
1
25.0%
BOV-OTHER
3
148
4
2.7%
BOV-P
6
795
23
2.9%
BOV-POSTM
1
8
1
12.5%
OTHER
1
48
4
8.3%
Pembrokeshire
18
207
30
14.5%
BOV-IR-C
56
582
104
17.9%

Country
County
Test Reason
Num Submissions Num Samples Tested Num Positive Samples % Positive
BOV-IR-EX
10
64
11
17.2%
BOV-OTHER
15
834
47
5.6%
BOV-P
10
325
23
7.1%
OTHER
2
18
1
5.6%
Powys
2
11
1
9.1%
BOV-IR-C
11
73
13
17.8%
BOV-IR-EX
2
35
7
20.0%
BOV-OTHER
2
107
18
16.8%
BOV-P
3
54
3
5.6%
South Wales
4
14
5
35.7%
BOV-IR-C
5
43
2
4.7%
BOV-P
1
67
0
0
Wales
229
4,413
429
9.7%
All GB
232
4,628
436
9.4 %