teleren/devto huggingface dataset:
Strategies for Gene Overexpression in Strain Engineering
Tags: webdev, beginners, tutorial
Published: 2023-09-08 03:49:36

The ability to overexpress genes in microorganisms has revolutionized the field of synthetic biology. Gene overexpression involves the production of large quantities of target proteins by increasing the transcription and translation of the gene of interest. This technique has been applied in the engineering of microorganisms to produce a wide range of products, including biofuels, chemicals, and biopharmaceuticals.

There are several strategies for [gene overexpression](https://www.biosynsis.com/gene-overexpression-in-strain-engineering.html) in strain engineering. One common method is [gene synthesis](https://www.biosynsis.com/genome-synthesis-and-assembly.html), which involves the design and synthesis of genes optimized for expression in specific host organisms. Gene experts modify the codon usage, remove destabilizing sequences, and add regulatory elements to enhance expression. Therefore, the target expression level of the gene is achieved through the modification and design of the gene.

Another approach is cloning. Experts utilize state-of-the-art techniques, such as restriction enzyme digestion and DNA ligation, polymerase chain reaction (PCR) amplification, plasmid-mediated transformation, and gene transfection, to clone synthesized genes into different expression vectors, enabling high levels of gene expression.  

In some cases, to enhance gene expression, regulatory elements that may reduce gene expression levels can be identified and removed. This type of gene optimization can be achieved by modifying the promoter, ribosome binding site, and other regulatory elements to obtain optimal gene expression levels.

Overall, gene overexpression is a powerful tool in strain engineering to enhance the production of specific compounds or metabolic activities. It involves increasing the expression level of specific genes through techniques such as gene synthesis, cloning, gene optimization, and [protein expression](https://www.biosynsis.com/recombinant-protein-expression-in-cho-cells.html). From a synthetic biology perspective, researchers can explore the role of gene overexpression in strain engineering and how they can optimize gene overexpression for diverse applications.
Crafting and Coding: My Open-Source Exploration in UI or AI
Published: 2023-09-08 03:45:46

Hello World! I'm Maryam, currently in my 4th year studying Software Development at Seneca College, Toronto. Open-source has always felt like this cool, giant puzzle to me where everyone adds their own piece, making something incredible together.

My heart has always had a spot for UI development.I mean, who doesn't love it when technology not only works but also looks good and feels right? There's a unique joy in crafting those pixel-perfect interfaces. But recently, AI has been calling out to me, merging logic with endless possibilities. This is why "Llama 2" and "Material UI" are on my radar. "Llama 2" is like diving into the deep end of the AI pool, thanks to Meta's innovation. And "Material UI"? That's where my UI dreams come to life, letting me play with colors, shapes, and interactions.

Join me as I juggle my love for both UI and AI this term. I'm excited to fit my own piece into this vast open-source puzzle and see the bigger picture unfold.
Methods for Gene Editing
Tags: webdev, beginners, tutorial
Published: 2023-09-08 03:43:42

Gene editing, one of the key tools in synthetic biology, allows scientists to manipulate the DNA of organisms to create new functions, optimize metabolic pathways, and engineer new traits. In recent years, the development of new gene editing tools, such as CRISPR-Cas9, has made gene editing faster, cheaper, and more precise than ever before.

Methods for [gene editing ](https://www.biosynsis.com/gene-editing.html)
CRISPR-Cas9
CRISPR-Cas9 is a revolutionary gene editing tool that uses a molecular complex composed of a guide RNA (gRNA) and a Cas9 protein. The gRNA guides the Cas9 protein to a specific location in the genome where it can make a double-stranded break. This break is then repaired by the cell's own DNA repair machinery, which can introduce desired genetic changes.

Multiplex Genome Editing by Natural Transformation (MuGENT) 
MuGENT is a gene editing method that utilizes the natural transformation process in bacterial cells to introduce multiple edits into the genome simultaneously. This technique is particularly useful for making multiple changes in the bacterial genome without the need for complex molecular biology techniques.

Genome synthesis
Genome synthesis is the process of creating artificial DNA sequences in a laboratory. This technique is highly valuable for gene editing as it allows scientists to design and engineer synthetic genes or whole genomes for various purposes, including gene therapy, drug development, and studying genetic diseases.

Homologous Recombination (HR)
Homologous recombination is a natural DNA repair process that can be harnessed for gene editing. In this method, a piece of DNA containing the desired genetic changes is introduced into the cell, and the cell uses its homologous recombination machinery to incorporate this DNA into the genome, replacing the target sequence.

Each of these gene editing methods has its advantages and limitations, and the choice of method depends on the specific requirements of the research or application. CD Biosynsis has years of experience in gene editing and [strain engineering](https://www.biosynsis.com/strain-engineering-services.html). Their experts use the latest tools and techniques to ensure that their services are precise, efficient, and cost-effective. Whether researchers are developing new strains for biotechnology, agriculture, or medicine, the company can help clients achieve their goals and drive innovation in their industry.
voice-swap - AI that works for artists
Tags: ai, singers
Published: 2023-09-08 03:39:01

Voice-Swap is the only platform that works with artists to explore next-gen fair payment models to monetise their presence in the age of AI. We have created a simple push-button licensing system that makes it easy to create demos with a subscription or trial and then pay to use them in your tracks.

We work with top global artists and have received glowing feedback from over 20,000 users, including producers like Diplo, Skream, Rob Swire, The Invisible Men, Beardyman, and many more.

Founded by multi-platinum producers turned software engineers DJ Fresh and Nico Pellerin, Voice-Swap focuses on production quality, creating the best vocal and singing models, whether for the platform publicly or for private clients.

[https://voice-swap.ai](https://voice-swap.ai)
Drawing Tool for Vue SAAS Application
Published: 2023-09-08 03:34:57

Hello All, 
we are building a SAAS application. One of the feature we are looking to incorporate is provide the users a tool for drawing. The tool should allow for the following:
1. Create straight and free hand line 
2. Ability to add text
3. Eraser
4. Adjust the thickness of the line
5. Change colors of lines
6. Brush feature for applying color
7. Ability to save the drawing and edit the drawing
Any feedback on plugin, preferably free that is compatible with Vue would be greatly appreciated. Thank you for your feedback.

AJ
Introducing supertext-react: Elevate Your Web Text Animations
Tags: react, animation, javascript, programming
Published: 2023-09-08 03:10:21

**Create Stunning Text Effects with Ease**

In the ever-evolving world of web development, enhancing user experiences has become paramount. One way to captivate your audience is through engaging animations, and text animations are no exception. Enter **supertext-react**, a powerful and flexible React component library designed to simplify the creation of captivating text animations.

### The Power of Text Animations

Text animations are a fantastic way to bring life to your web applications. They can convey information more dynamically, provide visual cues, and create memorable user interactions. However, crafting these animations from scratch can be time-consuming and complex.

That's where **supertext-react** comes in. With this library, you can effortlessly integrate various text animation effects into your React projects, allowing you to focus on delivering an exceptional user experience.

### Key Features

**supertext-react** offers a range of features and components tailored for text animations:

#### TypingText

Animate text as if it's being typed in real-time. Perfect for creating dynamic headers or simulating user interactions.

#### LastWordTypingText

Highlight the last word of a sentence, revealing it character by character. Ideal for emphasizing key points or adding a touch of creativity to your content.

#### Marquee

Add eye-catching scrolling text effects. Whether you want to display breaking news or showcase essential announcements, the Marquee component has you covered.

### Seamless Integration

Integrating **supertext-react** into your projects is straightforward. Simply install the library via npm or yarn and import the desired components into your React application. Then, customize the animations using props to match your design requirements.

```javascript
// Example import of TypingText component
import { TypingText } from 'supertext-react';
```

### Get Started Today

Ready to elevate your web text animations with **supertext-react**? Head over to our [npm package](https://www.npmjs.com/package/supertext-react) and [GitHub repository](https://github.com/abodmicheal/supertext-react) to access the library and documentation. You'll find detailed guides and examples to help you get started quickly.

Whether you're building a personal portfolio, a corporate website, or a cutting-edge web application, **supertext-react** empowers you to create memorable text animations that leave a lasting impression.

### Conclusion

In the competitive landscape of web development, delivering engaging user experiences is non-negotiable. **supertext-react** simplifies the process of adding captivating text animations to your React projects, allowing you to focus on what truly matters—creating an immersive and unforgettable digital journey for your users.

Get started with **supertext-react** today and unlock the power of text animations in your web applications.
WHAT EMOTIONS DOES ATTITUDE SHAYARI
Published: 2023-09-08 02:59:44

Attitude Shayari, often characterized by its assertive and bold language, evokes powerful emotions in its readers. Primarily, it instills a sense of empowerment and confidence, urging readers to embrace their individuality and express their thoughts without reservation. The sharp and witty language of attitude shayari can evoke amusement and delight, eliciting genuine smiles and even laughter from readers who resonate with its clever wordplay and audacious expressions. It also fosters a feeling of rebelliousness and defiance, challenging conventional norms and encouraging readers to question societal expectations.
**[Here](https://quirkyquillsq.blogspot.com/what-emotions-does-attitude-shayari)**

![Image description](https://dev-to-uploads.s3.amazonaws.com/uploads/articles/sop776a1r1jnj4m1e8oq.jpg)
Introduction
Tags: beginners, intro, opensource
Published: 2023-09-08 02:56:00

Hello There! 

My name is Yousef. I'm currently a Seneca College student in my final year. This semester, I've enrolled in the Topics in Open Source Development. I decided to take this course based on advice I received from experienced developers when I started my software development journey a few years ago. Which was to actively participate in open source projects. 

While exploring open source project repositories, I consistently found myself captivated by the concise documentation and clear instructions they provided. This happens to be an area where I've struggled personally. When the opportunity to take this class came up, I assumed that it would cover the creation of proper documentation for projects.

As I write this introductory blog post, my goals are to enhance my documentation skills, become more proficient in using GitHub and polish my GitHub account. I'm still uncertain about the specific projects I want to contribute to, but I'm leaning towards dotnet projects as I'd like to hone my skills in C#. My true passion lies in game development, and I believe that improving my C# skills will greatly benefit my career goals. Unfortunately, I haven't been able to identify a suitable open source game development projects for this course, but I'll certainly keep searching and provide updates in my future posts. 

The GitHub trending repository I've chosen is [DevToys](https://github.com/veler/DevToys). This tool is designed to streamline daily development tasks like text comparison and RegEx testing, among others. It even includes a smart feature that detects the best tool to use for clipboard data.

I picked this repository because many of the tools included in DevToys are ones I use regularly. I often find myself Googling and clicking on the first link that serves my purpose, whether it's for text comparison or JSON formatting. The concept of a tool that consolidates these functionalities into one package greatly appealed to me, so I installed it right away. Additionally, the fact that this is an open source project suggests that more tools will be added over time. This project is exciting, especially considering the project's well-designed user interface and usability. And since the project is written in C#, as I mentioned earlier, I'm keen to get more involved with C#.
HashiCorp's open-source exodus: Redefining DevOps in a Closed-Source Era
Tags: devops, terraform, automation, opensource
Published: 2023-09-08 02:47:23

https://cloudnativeengineer.substack.com/p/ep-8-hashicorps-open-source-exodus


![Image description](https://dev-to-uploads.s3.amazonaws.com/uploads/articles/u9jeaz49x8p2tbp9lo72.jpg)
Build LLM apps without coding. Langchain 🦜🔗
Tags: langchain, begginers, nocode, tutorial
Published: 2023-09-08 02:34:35

---
title: "Build LLM apps without coding. Langchain 🦜🔗"
description: "Setting up your Service account in Google Cloud."
tags: langchain, begginers, nocode, tutorial
series: Langchain without coding
---
A comprehensive guide on using Langflow and Flowise for building Langchain apps with Google's PALM2 LLM. 

This article starts a new series on langchain from the low-code/no-code perspective, where you'll learn how to create LLM apps using visual scripting.
If JavaScript is your territory, that would be Flowise, and for Python enthusiasts, the tool would be Langflow. 

Most of the Langchain guides on the internet use OpenAI's GPT3.5 or GTP4, but I will use Google's Palm2 in this series, given that you get 300 USD when you sign up for Google Cloud for the first time. 
I signed up in Canada and got 500+ CAD of credits; much more than the 5 USD you get for free with OpenAI.

You will need a service account with access to VertexAI; this is the identity under which Langchain will communicate with Google Cloud. Quick note: Using your primary Google Account for Langchain is a bad idea, as that account has god-level permissions on your Google Cloud project. Use a service account with limited permissions instead. 



## Enabling the Vertex AI API

1. Go to the [Google Cloud console](https://console.cloud.google.com/).
2. Click the **hamburger menu** in the top left corner of the page.
3. Select **APIs & Services** > **Library**.
4. Search for "Vertex AI."
5. Click the **Vertex AI API**.
6. Click **Enable**.

## Creating a service account in Google Cloud

1. Click the **hamburger menu** in the top left corner of the page.
2. Select **IAM & Admin** > **Service accounts**.
3. Click **Create service account**.
4. Enter a name for the service account. Click Create and Continue.
5. Grant the service account the **Vertex AI User** role.
6. (Optional) Enter a description for the service account.
7. Click **Done**.

Now, you'll see the list of service accounts available in your project for downloading the JSON Key of your newly created service account.

## Downloading the JSON key

1. Click the email address of the new service account.
2. Click the **Keys**tab.
3. Click the **Add key** drop-down menu.
4. Select **Create new key**.
5. Select **JSON**as the key type.
6. Click **Create**.
7. Download your key and store it **securely**.


**Note**: Anyone with this JSON file might use your service account on your behalf; do NOT share it online.

## Next Steps

[Create your first LLM app with Flowise(JS-based)
](https://dev.to/felipelujan/langchain-without-code-flowise-1l6a)

[WIP]Create your first LLM app with Flowise LangFlow(Python-based)
My first blog - Learn Open Source Development
Tags: beginners, opensource
Published: 2023-09-08 02:26:01

Hello, dear readers! I'm Yumei Wang, a student in [Seneca college] (https://www.senecacollege.ca/home.html). In this term I'm taking the course [Open Source Development](https://www.senecacollege.ca/cgi-bin/subject?s1=OSD600) because I believe I can gain more project experience by doing the real project.

My primary aim of learning OSD is to broaden my skill set by delving into backend development using Java. I've been working on things like React and React Native in my coop work. But now, I really want to understand how the stuff behind the scenes works, especially the backend part with Java. I want to be a full stack software engineer. I hope I can work on cool projects that show off what I've learned, and maybe even help others with my newfound knowledge. So, yeah, I'm excited to dive into this coding adventure and see where it takes me! 

To Start, I find an interesting project [mermaid] (https://github.com/mermaid-js/mermaid) by searching the [trend] (https://github.com/trending), which can draw awesome diagrams and charts by inputting basic docs. Also, there are lots of examples and guides on GitHub, so It's easy to learn and use. 

![Gantt chart](https://dev-to-uploads.s3.amazonaws.com/uploads/articles/4oaikvd7bhjyj3cw0g4x.png)
More Than Money: Finding Deeper Value in Coding
Tags: codenewbie, discuss, career, programming
Published: 2023-09-08 01:54:50

Hello everyone! Today, I want to ask a personal question: what do you find valuable about coding? 🤔

A lot of people, including a younger version of me, often venture into this sphere lured by the promise of six figure salaries and a full stack of benefits. It's undeniable; coding can unlock doors to financial stability and beyond, shaping dream careers and lifestyles. Yet, as someone who has always had a nerdy affection for science, I quickly developed a deeper passion for the craft. 🛠️

**But let's be real here**. The tech industry experienced an artificial boom during the pandemic, with many seeking easy pay and remote "work". This surge brought with it lots of opportunists only looking for a paycheck, and not willing to put in the actual work. I saw firsthand a good number of people giving the bare minimum, producing work that barely held together, if they produced any work at all. Sadly, this influx among many other factors had a pretty hard impact on the tech industry. 😓

But, the market is picking back up — there are opportunities out there for those who truly vibe with the art of coding. It's not as easy as it used to be, but hey, those who are passionate and take genuine pride in what they do are the ones still standing strong. 💪

We all know that just chasing the money leads to a shallow and short-lived joy, right? So, I gotta ask, what does coding really mean to you? Personally, I thrive on technical challenges and untangling complex problems. It's more than a job; it's a key that unlocks a world where I can bring my wildest dreams to life. 🌈

Growing up, I was that kid lost in daydreams, conjuring inventions wilder than a mad scientist’s. Cars? Nah, not my thing. But give me electronics and computers, and I was all in! My journey with coding started a bit late — small-town farm life didn't exactly come with coding manuals. Yet, the moment I dipped my toes in, a space in my heart I didn't even know was empty got filled up to the brim. 💖

Now, even with a day job I adore, my evenings and weekends are sacred times for exploration. Dive deep into topics from networking to tool creation; there's no end to this adventure! Building Sriracha UI, BestMarkdownEditor, and Davinci has been a ride — a small victory, yet the joy in the creation process? Absolutely unmatched. 🎢

Of course, not every project sees the light of day, but man, getting lost in that flow of creation is a kind of magic I just can't give up. Whether it’s jotting down my musings or crafting the next cool thing, the journey is where the true joy lies.

If any of this strikes a chord with you, come join the conversation on my [Discord](https://discord.gg/4PCy4Bz). I'm always around to chat or lend a helping hand! 🤝

Now, it’s over to you! I’m eager to hear your thoughts and stories. What pulls you into the world of coding? Share your perspective in the comments below!
Binary Search - Intro & Implementation
Tags: javascript, algorithms, codenewbie, beginners
Published: 2023-09-08 01:48:11

## Introduction

Binary search is a fundamental and highly efficient algorithm used to search for an element in a sorted array or list. It follows the principle of divide and conquer to rapidly locate the target element. In this guide, we'll introduce the binary search algorithm and provide a step-by-step implementation in JavaScript.

## How Binary Search Works

Binary search works by repeatedly dividing the search interval in half. It begins with the entire sorted array and compares the middle element to the target element. If the middle element matches the target, the search is successful. If the middle element is greater than the target, the search continues in the left half of the array, and if it's smaller, the search moves to the right half. This process repeats until the target is found or the search interval becomes empty.

## Algorithm Steps

1. Initialize two pointers, `left` and `right`, to the start and end of the array, respectively.
2. Calculate the middle index as `(left + right) / 2`.
3. Compare the middle element with the target:
   - If they match, the search is successful; return the middle index.
   - If the middle element is greater than the target, update `right` to `middle - 1` to search the left half.
   - If the middle element is smaller than the target, update `left` to `middle + 1` to search the right half.
4. Repeat steps 2-3 until the target is found or `left` becomes greater than `right`, indicating the target is not in the array.

## JavaScript Implementation

```javascript
function binarySearch(arr, target) {
  let left = 0;
  let right = arr.length - 1;

  while (left <= right) {
    const middle = Math.floor((left + right) / 2);

    if (arr[middle] === target) {
      return middle; // Target found
    } else if (arr[middle] < target) {
      left = middle + 1; // Search right half
    } else {
      right = middle - 1; // Search left half
    }
  }

  return -1; // Target not found
}

// Example usage:
const sortedArray = [2, 4, 6, 8, 10, 12, 14];
const target = 10;
const result = binarySearch(sortedArray, target);
if (result !== -1) {
  console.log(`Target ${target} found at index ${result}.`);
} else {
  console.log(`Target ${target} not found in the array.`);
}
```

## Time Complexity

Binary search has a time complexity of O(log n), where n is the number of elements in the sorted array. This makes it highly efficient, especially for large datasets, as it divides the search space in half with each comparison.

## Conclusion

Binary search is a powerful algorithm for quickly finding elements in a sorted array. Its efficiency makes it a fundamental tool in computer science and is widely used in various applications, including searching, data retrieval, and more. Understanding binary search and its implementation is essential for any programmer or software developer.