What is Bioconductor in R ?
Tags: bioinformatics, computationalbiology, bioconductor, r
Published: 2023-06-18 14:24:49

- Bioconductor is an open-source and open-development software project. It provides tools, packages, and resources for the analysis and comprehension of genomic data.

- Focuses on the statistical analysis and interpretation of high-throughput biological data.

- These packages include preprocessing, quality control, normalization, differential expression analysis, pathway analysis, genomic annotation, visualization, and machine learning.

> Bioconductor promotes collaboration and community contribution, with researchers and developers actively participating in the development and maintenance of packages.

- It emphasizes reproducible research by providing a platform for sharing and distributing analysis workflows, datasets, and methods.

- It incorporates important biological metadata and supports scalable software development.

- The project facilitates the exploration and interpretation of complex genomic datasets, enabling researchers to extract meaningful insights from their data.

__________________________________________________________

## How to Install Bioconductor ? 

1) `Install R` the programming language used for Bioconductor
2) To install core packages, type the following in an R command window
```
if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install()
```
3) Check Bioconductor version
```
version()
```
4) use `BiocManager::install()` to install specific packages e.g.
```
BiocManager::install("limma")
BiocManager::install(c("GenomicFeatures", "AnnotationDbi"))
```
5) Load Libraries using `library()`
```
Library(limma)
Library(GenomicFeatures)
Library(AnnotationDbi)
```
6) Display Information about the current R session
```
sessionInfo()
```

> When performing data analysis, it is important to document the versions of the software and packages used to ensure that the analysis can be reproduced in the future. Including `sessionInfo()` in your code, you can easily retrieve information about the versions of R and packages used at the time of analysis.

7) Check for package updates
```
valid()
```

___________________________________________________________

## Bioconductor VS Bioperl

![Image description](https://dev-to-uploads.s3.amazonaws.com/uploads/articles/w71wfh0razr5rarbb42e.png)

**Programming Language:**
- Bioconductor is primarily based on the R programming language. It provides a collection of R packages specifically designed for the analysis and comprehension of genomic data.

- Bioperl, on the other hand, is written in Perl, a general-purpose scripting language. It offers a comprehensive set of Perl modules for bioinformatics tasks. _(Decreased in Usage)_

**Scope and Focus:**

- Bioconductor is focused on the analysis of high-throughput genomic data, such as gene expression, DNA sequencing, and microarray data. It provides a wide range of packages for statistical analysis, visualization, and annotation of genomic data.

- Bioperl covers a broader range of bioinformatics tasks, including sequence analysis, molecular biology, and computational biology. It provides modules for parsing, manipulating, and analyzing biological sequence data, as well as tools for database access and integration.

**Ease of Use:**

- Bioconductor is known for its user-friendly interface and extensive documentation, making it accessible to both bioinformaticians and biologists with limited programming experience. The packages are designed to work well together, allowing users to easily combine multiple analyses.

- Bioperl is a powerful toolkit but can be more challenging for beginners due to its Perl programming language syntax. It requires a certain level of programming proficiency to effectively utilize and extend the toolkit. (Can be Harder)

**Integration with Other Tools:**

- Bioconductor is tightly integrated with R and leverages its extensive ecosystem of statistical and data manipulation packages. It also integrates well with other bioinformatics tools and resources, such as the NCBI databases and popular genome browsers.

- Bioperl provides interfaces to various external tools and databases, allowing users to seamlessly interact with external resources. It has built-in support for common file formats and can be easily integrated into bioinformatics pipelines.

## Major Bioconductor Packages

1. DESeq2: A tool for RNA-Seq data analysis, DESeq2 uses a negative binomial model to account for variability and identifies significant expression changes between conditions.

2. limma: A package for microarray data analysis, limma employs linear modeling and empirical Bayes methods to detect genes with significant expression differences between groups.

3. ShortRead: Specifically designed for short-read sequencing data analysis, ShortRead offers functions for quality control, alignment, read counting, variant calling, and other NGS-specific analyses within the Bioconductor framework.

4. edgeR: Another package for RNA-Seq analysis, edgeR utilizes a negative binomial distribution approach for differential gene expression analysis, including normalization, dispersion estimation, and identification of differentially expressed genes.

5. GenomicRanges: This package efficiently manipulates, annotates, and analyzes genomic intervals, providing operations like overlap detection, subsetting, merging, and visualization of genomic regions.

6. GenomicFeatures: Designed for genomic annotation data, GenomicFeatures facilitates the extraction, manipulation, and visualization of genomic features such as genes, transcripts, exons, and promoters.

## Example Using Zika Virus Dataset

![Image description](https://dev-to-uploads.s3.amazonaws.com/uploads/articles/gregw3gwzcbt24j4bg1u.jpg)

Zika_Virus_Dataset_from_Datacamp
 
- This is a simple code provided utilizes the Biostrings package, which is part of the Bioconductor project.

```
# Install and load the Biostrings package

library(Biostrings)

# Provide the path to the file
file_path <- "F:\\zika.txt"

# Read the file
zika_sequence <- readDNAStringSet(file_path)

# Check the length of the sequence
sequence_length <- width(zika_sequence)
cat("Sequence Length:", sequence_length, "\n")

#--- output --- : Sequence Length: 10794

# Retrieve the first 50 characters of the sequence (if available)
if (sequence_length >= 50) {
  first_50_chars <- as.character(zika_sequence)[1:50]
  cat("First 50 Characters:", first_50_chars, "\n")
} else if (sequence_length > 0) {
  first_50_chars <- as.character(zika_sequence)[1:sequence_length]
  cat("First", sequence_length, "Characters:", first_50_chars, "\n")
} else {
  cat("No sequence data available.\n")
}

#--- output --- : First 50 Characters:AGTTGTTGATCTGTGTGAGTCAGACTGCGACA----

# Count the number of occurrences of a specific subsequence
subsequence <- DNAString("AGTT")
subsequence_count <- vcountPattern(subsequence, zika_sequence)
cat("Subsequence Count:", subsequence_count, "\n")

#--- output --- : Subsequence Count: 34

# DNA single string
dna_seq <- DNAString("ATGATCTCGTAA")
print("DNA sequence:")
print(dna_seq)

"""
--- output --- :
DNA sequence:
12-letter DNAString object
seq: ATGATCTCGTAA
"""
# Transcription DNA to RNA string
rna_seq <- RNAString(dna_seq)
print("RNA sequence:")
print(rna_seq)

"""
--- output --- :
RNA sequence:
12-letter RNAString object
seq: AUGAUCUCGUAA
"""

# Translation RNA to amino acids
print("Translation RNA to amino acids:")
aa_seq <- translate(rna_seq)
print(aa_seq)

"""
--- output --- :
Translation RNA to amino acids:
4-letter AAString object
seq: MIS*
"""

# Shortcut translate DNA to amino acids
print("Shortcut translate DNA to amino acids:")
aa_seq_shortcut <- translate(dna_seq)
print(aa_seq_shortcut)

"""
--- output --- : 
Shortcut translate DNA to amino acids:
4-letter AAString object
seq: MIS*
"""

# Read the dataset from the file
dataset <- readLines(file_path)
# Combine the lines into a single string
dataset <- paste(dataset, collapse = "")
# Define the pattern
pattern <- "GGG"

# Calculate the frequency of the pattern within the dataset
pattern_count <- sum(gregexpr(pattern, dataset, fixed = TRUE)[[1]] > 0)
# Print the pattern count
print(pattern_count)

#--- output --- : 171
```

________________________________________________________

> In conclusion, Bioconductor is a powerful and widely used software project in R that provides a comprehensive collection of packages and resources for analyzing genomic data. It offers a range of tools and algorithms for tasks such as quality control, preprocessing, differential expression analysis, pathway analysis, and visualization. Bioconductor stands out for its extensive package ecosystem, with specialized functionality covering various areas of genomics.