tbuckley/cabot-search huggingface dataset:
Identification of a molecular resistor that controls UCP1-independent Ca2+ cycling thermogenesis in adipose tissue
Authors: Christopher Auger, Mark Li, Masanori Fujimoto, Kenji Ikeda, Jin-Seon Yook, Timothy R O'Leary, María Paula Huertas Caycedo, Cai Xiaohan, Satoshi Oikawa, Anthony R P Verkerke, Kosaku Shinoda, Patrick R Griffin, Kenji Inaba, Roland H Stimson, Shingo Kajimura
Journal: Cell Metabolism (2025)
Mapping genetic diversity with the GenomeIndia project
Authors: Chandrika Bhattacharyya, Krithika Subramanian, Bharathram Uppili, Nidhan K Biswas, Shweta Ramdas, Karthik Bharadwaj Tallapaka, Prathima Arvind, Khader Valli Rupanagudi, Arindam Maitra, Tulasi Nagabandi, Tiyasha De, Kuldeep Singh, Praveen Sharma, Nanaocha Sharma, Sunil K Raghav, Punit Prasad, E V Soniya, Abdul Jaleel, Shijulal Nelson Sathi, Madhvi Joshi, Chaitanya Joshi, Mayurika Lahiri, Santosh Dixit, L S Shashidhara, Nachimuthu Senthil Kumar, H Lalhruaitluanga, Lal Nundanga, Venkataram Shivakumar, Ganesan Venkatasubramanian, Naren P Rao, Mohd Ashraf Ganie, Imtiyaz Ahmad Wani, Ganganath Jha, Ashwin Dalal, Murali Dharan Bashyam, Pritish Kumar Varadwaj, Sanjeev Bs, Yogesh Simmhan, Chirag Jain, Durai Sundar, Ishaan Gupta, Pankaj Yadav, Himanshu Sinha, Manikandan Narayanan, Karthik Raman, Raghu Padinjat, Radhakrishnan Sabarinathan, Yadati Narahari, Vijayalakshmi Ravindranath, Thangaraj Kumarasamy, Divya Tej Sowpati, Mohammed Faruq, Analabha Basu, Bratati Kahali
Journal: Nature Genetics (2025)
Microbiome–metabolome dynamics associated with impaired glucose control and responses to lifestyle changes
Authors: Hao Wu, Bomin Lv, Luqian Zhi, Yikai Shao, Xinyan Liu, Matthias Mitteregger, Rima Chakaroun, Valentina Tremaroli, Stanley L Hazen, Ru Wang, Göran Bergström, Fredrik Bäckhed
Journal: Nature Medicine (2025)

Abstract Type 2 diabetes (T2D) is a complex disease shaped by genetic and environmental factors, including the gut microbiome. Recent research revealed pathophysiological heterogeneity and distinct subgroups in both T2D and prediabetes, prompting exploration of personalized risk factors. Using metabolomics in two Swedish cohorts ( n = 1,167), we identified over 500 blood metabolites associated with impaired glucose control, with approximately one-third linked to an altered gut microbiome. Our findings identified metabolic disruptions in microbiome–metabolome dynamics as potential mediators of compromised glucose homeostasis, as illustrated by the potential interactions between Hominifimenecus microfluidus and Blautia wexlerae via hippurate. Short-term lifestyle changes, for example, diet and exercise, modulated microbiome-associated metabolites in a lifestyle-specific manner. This study suggests that the microbiome–metabolome axis is a modifiable target for T2D management, with optimal health benefits achievable through a combination of lifestyle modifications.
Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity
Authors: Xingyu Zhou, Dingchao Zhu, Di Wu, Gaocai Li, Huaizhen Liang, Weifeng Zhang, Yali Wu, Hanpeng Xu, Zhengdong Zhang, Bide Tong, Yu Song, Kun Wang, Xiaobo Feng, Jie Lei, Hongchuan Wang, Xiaoguang Zhang, Liang Ma, Yuhang Chen, Junyu Wei, Zixuan Ou, Shuchang Peng, Xinghuo Wu, Lei Tan, Bingjin Wang, Cao Yang
Journal: Cell Reports Medicine (2025)
Genetic Risk and First-Trimester Cardiovascular Health Predict Hypertensive Disorders of Pregnancy in Nulliparous Women
Authors: Vineetha Mathew, Raiyan R Khan, Amanda R Jowell, Qi Yan, Itsik Pe'er, Buu Truong, Pradeep Natarajan, Lynn M Yee, Sadiya S Khan, Garima Sharma, Aniruddh P Patel, So Mi Jemma Cho, Maria A Pabon, Rebecca B McNeil, Jillyn Spencer, Robert M Silver, Lisa D Levine, William A Grobman, Janet M Catov, David M Haas, Michael C Honigberg
Journal: Journal of the American College of Cardiology (2025)
Technology-assisted cognitive-behavioral therapy for perinatal depression delivered by lived-experience peers: a cluster-randomized noninferiority trial
Authors: Atif Rahman, Abid Malik, Huma Nazir, Ahmed Zaidi, Anum Nisar, Ahmed Waqas, Najia Atif, Naomi Kate Gibbs, Yutian Luo, Siham Sikander, Duolao Wang
Journal: Nature Medicine (2025)
TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux
Authors: Joanne F Garbincius, Oniel Salik, Henry M Cohen, Carmen Choya-Foces, Adam S Mangold, Angelina D Makhoul, Anna E Schmidt, Dima Y Khalil, Joshua J Doolittle, Anya S Wilkinson, Emma K Murray, Michael P Lazaropoulos, Alycia N Hildebrand, Dhanendra Tomar, John W Elrod
Journal: Nature Metabolism (2025)
Genetic regulation of gene expression across multiple tissues in chickens
Authors: Dailu Guan, Zhonghao Bai, Xiaoning Zhu, Conghao Zhong, Yali Hou, Di Zhu, Houcheng Li, Fangren Lan, Shuqi Diao, Yuelin Yao, Bingru Zhao, Xiaochang Li, Zhangyuan Pan, Yahui Gao, Yuzhe Wang, Dong Zou, Ruizhen Wang, Tianyi Xu, Congjiao Sun, Hongwei Yin, Jinyan Teng, Zhiting Xu, Qing Lin, Shourong Shi, Dan Shao, Fabien Degalez, Sandrine Lagarrigue, Ying Wang, Mingshan Wang, Minsheng Peng, Dominique Rocha, Mathieu Charles, Jacqueline Smith, Kellie Watson, Albert Johannes Buitenhuis, Goutam Sahana, Mogens Sandø Lund, Wesley Warren, Laurent Frantz, Greger Larson, Susan J Lamont, Wei Si, Xin Zhao, Bingjie Li, Haihan Zhang, Chenglong Luo, Dingming Shu, Hao Qu, Wei Luo, Zhenhui Li, Qinghua Nie, Xiquan Zhang, Ruidong Xiang, Shuli Liu, Zhe Zhang, Zhang Zhang, George E Liu, Hans Cheng, Ning Yang, Xiaoxiang Hu, Huaijun Zhou, Lingzhao Fang
Journal: Nature Genetics (2025)
Ultrasound‐Mediated Biomimetic Microbubbles Effectively Reverse LSECs Capillarization and Exert Antiplatelet Therapy in Liver Fibrosis
Authors: Huan Deng, Huolin Ye, Hong Xiao, Yujia You, Xiaoyan Miao, Wei Zhang, Yifei Leng, Ronqin Zheng, Xintao Shuai, Jie Ren, Tinhui Yin
Journal: Advanced Healthcare Materials (2025)

Abstract Liver fibrosis, characterized by excessive tissue remodeling as a response to chronic liver injury, is accompanied by capillarization of liver sinusoidal endothelial cells (LSECs) and activated hepatic stellate cells (HSCs). Simvastatin (Sim) can modulate endothelial function by increasing endothelial nitric oxide synthase (eNOS)‐dependent nitric oxide (NO) release, thereby reversing capillarization and attenuating liver fibrosis. However, monotherapy often demonstrates limited therapeutic effectiveness given the complex pathophysiology of liver fibrosis. Herein, a type of multifunctional liposomal microbubbles (MBs) carrying both Sim and platelet membrane (PM) has been designed for drug delivery targeting the inflammatory LSECs, with ultrasound‐targeted microbubble destruction (UTMD) to mediate efficient release of these therapeutic agents inside the liver sinusoidal. In rat liver fibrosis model, the multifunctional MBs reverses capillarization through the increase of eNOS‐dependent NO production. Subsequently, the MBs adhering to the inflammatory LSECs block the adhesion and activation of inherent platelet (PLT), thereby decreasing platelet‐derived growth factor β (PDGF‐β) to inhibit the HSCs activation. This study demonstrates the strong therapeutic efficacy of the multifunctional MBs integrating Sim and PLT against liver fibrosis, which highlights a great potential for effectively managing this intractable chronic disease.
Single-cell genomics of the mouse olfactory cortex reveals contrasts with neocortex and ancestral signatures of cell type evolution
Authors: Sara Zeppilli, Alonso O Gurrola, Pinar Demetci, David H Brann, Tuan M Pham, Robin Attey, Noga Zilkha, Tali Kimchi, Sandeep R Datta, Ritambhara Singh, Maria A Tosches, Anton Crombach, Alexander Fleischmann
Journal: Nature Neuroscience (2025)
A Wireless Cortical Surface Implant for Diagnosing and Alleviating Parkinson's Disease Symptoms in Freely Moving Animals
Authors: Hongseong Shin, Kyungtae Kim, Jaeseung Lee, Johyeon Nam, Eunha Baeg, Chaeyeon You, Hanseul Choi, Minji Kim, Chun Kee Chung, Jae Geun Kim, Jong Hyun Ahn, Miryung Han, Jibum Kim, Sungchil Yang, Sung Q Lee, Sunggu Yang
Journal: Advanced Healthcare Materials (2025)

Abstract Parkinson's disease (PD), one of the most common neurodegenerative diseases, is involved in motor abnormality, primarily arising from the degeneration of dopaminergic neurons. Previous studies have examined the electrotherapeutic effects of PD using various methodological contexts, including live conditions, wireless control, diagnostic/therapeutic aspects, removable interfaces, or biocompatible materials, each of which is separately utilized for testing the diagnosis or alleviation of various brain diseases. Here, a cortical surface implant designed to improve motor function in freely moving PD animals is presented. This implant, a minimally invasive system equipped with a graphene electrode array, is the first integrated system to exhibit biocompatibility, wearability, removability, target specificity, and wireless control. The implant positioned at the motor cortical surface activates the motor cortex to maximize therapeutic effects and minimize off‐target effects while monitoring motor activities. In PD animals, cortical motor surface stimulation restores motor function and brain waves, which corresponds to potentiated synaptic responses. Furthermore, these changes are associated with the upregulation of metabotropic glutamate receptor 5 (mGluR5, Grm5 ) and D5 dopamine receptor (D 5 R , Drd5 ) genes in the glutamatergic synapse. The newly designed wireless neural implant demonstrates capabilities in both real‐time diagnostics and targeted therapeutics, suggesting its potential as a wireless system for biomedical devices for patients with PD and other neurodegenerative diseases.
Biophysically Optimized Nanofiber‐Hydrogel Scaffold Composite Acts as “Bio‐Bonsai” for Peripheral Nerve Simulation and Regeneration via Orienting Adipose Derived Stem Cells into Schwann‐Like Cell Differentiation
Authors: Yanrong Qin, Bo Chen, Yubin Hu, Xiyu Zhang, Zihan Wang, Chengjie Ma, Ruishan Yang, Bang Wang, Fan Li, Shiwei Niu, Yi Han, Di Lu
Journal: Advanced Healthcare Materials (2025)

Abstract Efficient repairment of peripheral nerve injury (PNI) remains a severe clinical challenge worldwide, and recovering the regenerative capability of neurons in peripheral nervous system is hindered by the slow rate and inaccurate direction of axonal elongation. Schwann cells (SCs) loaded nerve guidance conduit has been proven to improve PNI repair, but the low cell survival rate and incomplete differentiation of SCs limited its practical application. To address these hurdles, a biophysically optimized nanofiber‐hydrogel scaffold composite (APML@PC) is prepared in this study, the “bio”bonsai“”inspired strategy integrates topological and biological cues to promote adipose‐derived mesenchymal stem cells (ADSCs) adhesion, proliferation, and Schwann‐like cell differentiation. In vitro and in vivo experiments confirmed the favorable biocompatibility and reasonable biodegradation behavior of this inducible platform, and the robust capability to promote axonal growth, remyelination regeneration, as well as nerve function recovery. This novel composite can serve as a promising candidate for the development of advanced stem cell‐based peripheral nerve regeneration, thereby paving a new avenue for clinically effective PNI therapy.
Nitric Oxide‐Mediated Dual‐Functional Smart Titanium Implant Coating for Antibacterial and Osseointegration Promotion in Implant‐Associated Infections
Authors: Zishuo Hou, Kun Wang, Guming Liu, Zhang Yuan, Haowei Peng, Yue Yuan, Hongbo Wei, Tengjiao Wang, Peng Li
Journal: Advanced Healthcare Materials (2025)

Abstract The balance of antibacterial and osseointegration‐promoting properties on titanium (Ti) implants in a simple and efficient manner is crucial for the management of implant‐associated infections, a condition that has become a significant global health concern. Herein, a nitric oxide (NO)‐mediated dual‐function smart coating with antibacterial and osseointegration‐promoting properties is developed for Ti implants. The coating leverages the distinct properties of NO at high and low concentrations to enable an on‐demand functional switch. Specifically, antibacterial function is achieved through a rapid release of high‐dose NO in response to the infection microenvironment and near‐infrared stimulation. Once the infection is resolved and normal physiological conditions are restored, the coating gradually releases low‐dose NO to promote osseointegration. In vitro tests confirm that the coating exhibits antibacterial ratio of 97.84% and 97.18% against methicillin‐resistant Staphylococcus aureus and its biofilms, respectively, and demonstrates the ability to activate osteoblasts. The rat femoral implant‐associated infection model further certifies that the responsive NO release mechanism of the coating efficiently facilitates the on‐demand functional switch between antibacterial and osseointegration‐promoting properties. Notably, the use of the dual‐functional nonantibiotic agent, NO, significantly mitigates the risk of bacterial resistance.
The choice of study designs of diagnostic accuracy using Borrelia specific IgG and IgM antibodies for the diagnosis of Lyme borreliosis
Authors: Ram Benny Dessau, Alice Raffetin, Randi Eikeland, Volker Fingerle, Anna J Henningsson, Klaus-Peter Hunfeld, Benoit Jaulhac, Reto Lienhard, Per-Eric Lindgren, Mateusz Markowicz, Sally Mavin, Katharina Ornstein, Michiel Wijnveld, Franc Strle
Journal: Clinical Microbiology and Infection (2025)
Tissue‐Adhesive, Antibacterial, and Antioxidant Hydrogel Sealant for Sealing Colorectal Anastomotic Leakage and Preventing Postoperative Adhesion
Authors: Zhen Wang, Shuang Li, Desheng Qi, Yang Gao, Yujia Geng, Zheng Zou, Zhen Zhang, Chaoliang He, Quan Wang
Journal: Advanced Healthcare Materials (2025)

Abstract Surgical treatment of colorectal diseases typically involves excising the diseased portion of the bowel and anastomosing the remaining sections to reestablish continuity. Surgical suturing has limitations in preventing anastomotic leakage and postoperative adhesion. To address these challenges, a tissue‐adhesive, antibacterial, and antioxidant hydrogel is designed to cover and seal colorectal anastomotic wounds. The hydrogel is formed in situ by simply mixing oxidized hyaluronic acid, adipic acid dihydrazide‐modified hyaluronic acid, ε‐poly‐ l ‐lysine, and tannic acid. The hydrogel exhibits a rapid gelation rate and self‐healing ability. Compared with commercial fibrin glue, the hydrogel has superior tissue‐adhesive strength and wound sealing performance. The hydrogel displays potent reactive oxygen species scavenging ability and antibacterial activity against both Gram‐positive and Gram‐negative bacteria. The hydrogel also exhibits good biodegradation and biocompatibility. In a cecum‐abdominal wall adhesion model in rats, the hydrogel attaches firmly to the injured tissues and serves as a physical barrier to prevent adhesion formation. In anastomotic leakage models after colon resection in rats and rabbits, the hydrogel effectively seals the anastomotic leakage, prevents postoperative adhesion, and promotes anastomotic healing. Thus, this multifunctional hydrogel has strong clinical potential for preventing anastomotic leakage and adhesion formation after colorectal surgery.
Glycogen-inspired trimannosylated serum albumin nanocarriers for targeted delivery of toll-like receptor 7/8 agonists to immune cells and liver
Authors: Bellinda Lantzberg, Yanira Zeyn, Robert Forster, Lin Jian, Dominik Schauenburg, Christoph Hieber, Lutz Nuhn, Tianjiao Zhou, Maria J S A Silva, Kaloian Koynov, Hu-Lin Jiang, Seah Ling Kuan, Matthias Bros, Till Opatz, Tanja Weil
Journal: Journal of Controlled Release (2025)
Mechanical activation of adipose tissue macrophages mediated by Piezo1 protects against diet-induced obesity by regulating sympathetic activity
Authors: Shaoqiu Leng, Xiaoyu Zhang, Ruxia Zhao, Nan Jiang, Xinyue Liu, Xin Li, Qi Feng, Zi Sheng, Shuwen Wang, Jun Peng, Xiang Hu
Journal: Metabolism (2025)
ESMO Precision Oncology Working Group recommendations on the structure and quality indicators for molecular tumour boards in clinical practice
Authors: C B Westphalen, L Boscolo Bielo, P Aftimos, H Beltran, M Benary, D Chakravarty, M Collienne, R Dienstmann, A El Helali, J Gainor, P Horak, C Le Tourneau, C Marchiò, C Massard, F Meric-Bernstam, C Pauli, G Pruneri, F Roitberg, H E G Russnes, D B Solit, N Starling, V Subbiah, D Tamborero, N Tarazona, C Turnbull, J van de Haar, F André, J Mateo, G Curigliano
Journal: Annals of Oncology (2025)

With an increased uptake of genomic profiling in clinical practice and the evolving complexity of diagnostic modalities, vast amounts of complex data need to be properly interpreted and integrated into an individualised care plan. To address these challenges, molecular tumour boards (MTBs) have been widely established. As of today, no international recommendations regulating the composition and workflows of MTBs have been defined. ESMO's Precision Oncology Working Group (POWG) established an international expert panel in precision oncology and defined core areas of interest. After several consultations and through an expert consensus process, the group reached a consensus level for each recommendation. The group defined five components in the MTB process that are critical to its function and clinical use: (i) the primary task of MTBs consists in providing genomic-informed clinical recommendations, particularly for cases exhibiting complex genomic alterations; (ii) to achieve this, MTBs should encompass interdisciplinary expertise, with key roles for oncologists with genomic expertise, pathologists with molecular training and clinical geneticists; (iii) MTBs' recommendations should be documented in a structured report that includes genomic-informed treatment strategies, management plans for potential tumour-detected germline alterations and guidance for additional genomic testing; (iv) structured follow-up processes should be implemented for monitoring the clinical effectiveness of MTBs recommendations and (v) finally, the panel proposed quality indicators for operating MTBs, including turnaround times for cases discussion and the proportion of cases for which actionable recommendations and clinical trial enrolments were successfully implemented. These ESMO's POWG recommendations can serve as a guidance and help to define quality standards for MTBs to allow for harmonisation and to further expedite the integration of precision oncology into clinical practice.
Gender and global health: going, going, but not gone
Authors: Sarah Hawkes, Raewyn Connell, Jocalyn Clark, Jeni Klugman, Gary L Darmstadt, Erica Nelson, Amy Chiaying Hsieh, Elhadj As Sy, Gary Barker, Beniamino Cislaghi, Kent Buse
Journal: The Lancet (2025)
Health outcomes up to 3 years and post-exertional malaise in patients after hospitalization for COVID-19: a multicentre prospective cohort study (CO-FLOW)
Authors: Julia C. Berentschot, L. Martine Bek, Manon Drost, Rita J.G. van den Berg-Emons, Gert-Jan Braunstahl, Gerard M. Ribbers, Joachim G.J.V. Aerts, Merel E. Hellemons, Majanka H. Heijenbrok-Kal, Joachim G.J.V. Aerts, L. Martine Bek, Julia C. Berentschot, Rita J.G. van den Berg-Emons, Sieshem Bindraban, Wouter J.B. Blox, Jasper van Bommel, Shai A. Gajadin, Michel E. van Genderen, Diederik A.M.P.J. Gommers, Majanka H. Heijenbrok-Kal, Merel E. Hellemons, Roxane Heller, Erwin Ista, Stephanie van Loon-Kooij, Chantal Luijkx, Rutger Osterthun, Laurien Oswald, Gerard M. Ribbers, Ronald N. van Rossem, Herbert J. van de Sande, Robert van der Stoep, Janette J. Tazmi-Staal, Markus P.J.M. Wijffels, Eva G. Willems
Journal: The Lancet Regional Health - Europe (2025)
Endovascular treatment for cerebral venous thrombosis: a multicenter study in China
Authors: He-Tao Bian, Xia Wang, Gui-You Liu, Chen Zhou, Ran Meng, Lan Liu, Jian-Gang Duan, Feng Yan, Chuan-Hui Li, Min Li, Wen Hui, Xu-Xiang Zhang, Dong Zhao, Ya-Peng Li, Qi Fang, De-Zhi Kang, Hong-Liang Zeng, Zhi-Jian Liang, Zheng-Hao Shi, Wei Yue, Qin-Jian Sun, Gui-Sheng Chen, Jian-Long Song, Zhong-Rui Yan, Qiu-Hong Ji, Kai-Jie Wang, Lu-Sha Tong, Xiao Hu, Wen-Feng Cao, Wei Yan, Rui-Jiang Gao, Qi Li, Jian-Yi Wang, Yi Liu, Bao-Jun Wang, Xiao-Hua Wang, Sheng-Tao Yao, Ye Lang, Hai-Peng Li, Craig S Anderson, Xun-Ming Ji
Journal: Military Medical Research (2025)

Abstract Background Endovascular treatment (EVT) is gaining popularity for the management of severe forms of cerebral venous thrombosis (CVT), but the evidence supporting its efficacy and safety is limited. Methods This multicenter study included patients with CVT admitted to 104 hospitals in 31 provinces/cities in China between January 2018 and June 2022. Propensity score weighting models were used to adjust baseline confounding variables to determine the association of EVT on the primary outcome of good functional status, defined as score 0 − 1 on the modified Rankin Scale after hospital discharge. Results Of 3063 patients identified through hospital records searches, 2774 adults [age (42 ± 15.8) years, female 50.3%] fulfilled eligibility criteria and agreed to be included, of whom 449 (16.2%) received EVT and 2325 (83.8%) received standard care. There was no significant difference between the EVT group and the standard care group in terms of the possibility of good functional recovery [weighted risk ratio = 1.00, 95% confidence interval (CI) 0.96 − 1.03]. Similarly, there was no difference in the likelihood of death at hospital discharge (weighted risk ratio = 1.91, 95% CI 0.91 − 3.68). In subgroup analysis, the possibility of good functional recovery was lower in patients with intracerebral hemorrhage (weighted risk ratio = 0.88, 95% CI 0.79 − 0.98; P for interaction = 0.01) and seizures (weighted risk ratio = 0.86, 95% CI 0.76 − 0.95; P for interaction = 0.03). Conclusion In this large nationwide study, EVT was not associated with improved functional outcomes compared to standard care in patients with CVT.
3'-Sialyllactose and <i>B. infantis</i> synergistically alleviate gut inflammation and barrier dysfunction by enriching cross-feeding bacteria for short-chain fatty acid biosynthesis
Authors: Mingzhi Yang, Zipeng Jiang, Lutong Zhou, Nana Chen, Huan He, Wentao Li, Zhixin Yu, Siming Jiao, Deguang Song, Yizhen Wang, Mingliang Jin, Zeqing Lu
Journal: Gut Microbes (2025)

Ulcerative colitis (UC) poses significant threats to human health and quality of life worldwide, as it is a chronic inflammatory bowel disease. 3'-sialyllactose (3'-SL) is a key functional component of milk oligosaccharides. This study systematically evaluates the prebiotic effects of 3'-SL and its therapeutic potential in combination with Bifidobacterium infantis (B. infantis) for UC. The findings reveal that 3'-SL and B. infantis synergistically mitigate intestinal inflammation and barrier dysfunction by promoting the production of short-chain fatty acids (SCFAs) through cross-feeding mechanisms among gut microbiota. Individually, 3'-SL, B. infantis, and the synbiotic treatment all effectively alleviated UC symptoms, including reduced weight loss, improved disease activity scores, and prevention of colon shortening. Histopathological and immunofluorescence analyses further demonstrated that the synbiotic treatment significantly ameliorated colonic injury, enhanced barrier function, restored goblet cell counts, increased glycoprotein content in crypt goblet cells, and upregulated the expression of tight junction proteins (ZO-1, occludin, and claudin-1). Notably, the synbiotic treatment outperformed the individual components by better restoring gut microbiota balance, elevating SCFA levels, and modulating serum cytokine profiles, thereby reducing inflammation. These findings provide mechanistic insights into the protective effects of the synbiotic and underscore its therapeutic potential for UC and other intestinal inflammatory disorders.