tbuckley/cabot-search huggingface dataset:
Lung‐Mimetic Hydrofoam Sealant to Treat Pulmonary Air Leak
Authors: Meghan R. Pinezich, Mohammad Mir, Pamela L. Graney, Daniel Naveed Tavakol, Jiawen Chen, Maria R. Hudock, Olimpia Gavaudan, Panpan Chen, Sarah R. Kaslow, Jonathan A. Reimer, Julie Van Hassel, Brandon A. Guenthart, John D. O'Neill, Matthew Bacchetta, Jinho Kim, Gordana Vunjak‐Novakovic
Journal: Advanced Healthcare Materials (2024)

Abstract Pulmonary air leak is the most common complication of lung surgery, contributing to post‐operative morbidity in up to 60% of patients; yet, there is no reliable treatment. Available surgical sealants do not match the demanding deformation mechanics of lung tissue; and therefore, fail to seal air leak. To address this therapeutic gap, a sealant with structural and mechanical similarity to subpleural lung is designed, developed, and systematically evaluated. This “lung‐mimetic” sealant is a hydrofoam material that has alveolar‐like porous ultrastructure, lung‐like viscoelastic properties (adhesive, compressive, tensile), and lung extracellular matrix‐derived signals (matrikines) to support tissue repair. In biocompatibility testing, the lung‐mimetic sealant shows minimal cytotoxicity and immunogenicity in vitro. Human primary monocytes exposed to sealant matrikines in vitro upregulate key genes (MARCO, PDGFB, VEGF) known to correlate with pleural wound healing and tissue repair in vivo. In rat and swine models of pulmonary air leak, this lung‐mimetic sealant rapidly seals air leak and restores baseline lung mechanics. Altogether, these data indicate that the lung‐mimetic sealant can effectively seal pulmonary air leak and promote a favorable cellular response in vitro.
Understanding the mechanisms of diffuse midline glioma cell migration toward therapeutic targeting
Authors: Stephen C Mack, Kelsey C Bertrand
Journal: Neuro-Oncology (2024)

Journal Article Understanding the mechanisms of diffuse midline glioma cell migration toward therapeutic targeting Get access Stephen C Mack, Stephen C Mack Department of Developmental Neurobiology, St Jude Children's Research Hospital, Memphis, Tennessee, USANeurobiology and Brain Tumor Program, St Jude Children's Research Hospital, Memphis, Tennessee, USA Corresponding Author:stephen.mack@stjude.org https://orcid.org/0000-0001-9620-4742 Search for other works by this author on: Oxford Academic PubMed Google Scholar Kelsey C Bertrand Kelsey C Bertrand Neurobiology and Brain Tumor Program, St Jude Children's Research Hospital, Memphis, Tennessee, USADepartment of Oncology, St Jude Children's Research Hospital, Memphis, Tennessee, USA Corresponding Author:kelsey.bertrand@stjude.org Search for other works by this author on: Oxford Academic PubMed Google Scholar Neuro-Oncology, Volume 26, Issue 3, March 2024, Pages 569–570, https://doi.org/10.1093/neuonc/noad263 Published: 27 January 2024 Article history Published: 27 January 2024 Corrected and typeset: 08 February 2024
Fabric‐Enhanced Vascular Graft with Hierarchical Structure for Promoting the Regeneration of Vascular Tissue
Authors: Wenxin Ma, Zhuo Liu, Tonghe Zhu, Liming Wang, Juan Du, Kun Wang, Chen Xu
Journal: Advanced Healthcare Materials (2024)

Abstract Natural blood vessels have completed functions, including elasticity, compliance, and excellent antithrombotic properties because of their mature structure. To replace damaged blood vessels, vascular grafts should perform these functions by simulating the natural vascular structures. Although the structures of natural blood vessels are thoroughly explored, constructing a small‐diameter vascular graft that matches the mechanical and biological properties of natural blood vessels remains a challenge. A hierarchical vascular graft is fabricated by Electrospinning, Braiding, and Thermally induced phase separation (EBT) processes, which could simulate the structure of natural blood vessels. The internal electrospun structure facilitates the adhesion of endothelial cells, thereby accelerating endothelialization. The intermediate PLGA fabric exhibits excellent mechanical properties, which allow it to maintain its shape during long‐term transplantation and prevent graft expansion. The external macroporous structure is beneficial for cell growth and infiltration. Blood vessel remodeling aims to combine a structure that promotes tissue regeneration with anti‐inflammatory materials. The results in vitro demonstrated that it EBT vascular graft (EBTVG) has matched the mechanical properties, reliable cytocompatibility, and the strongest endothelialization in situ. The results in vitro and replacement of the resected artery in vivo suggest that the EBTVG combines different structural advantages with biomechanical properties and reliable biocompatibility, significantly promoting the stabilization and regeneration of vascular endothelial cells and vascular smooth muscle cells, as well as stabilizing the blood microenvironment.
CDK6 protein expression is associated with disease progression and treatment resistance in multiple myeloma
Authors: Johannes Steinhart, Peter Möller, Miriam Kull, Jan Krönke, Thomas F. E. Barth
Journal: HemaSphere (2024)

Abstract Multiple myeloma (MM) is a heterogeneous malignancy of plasma cells. Despite improvement in the prognosis of MM patients after the introduction of many new drugs in the past decades, MM remains incurable since most patients become treatment‐resistant. Cyclin‐dependent kinase 6 (CDK6) is activated in many types of cancer and has been associated with drug resistance in MM. However, its association with disease stage, genetic alterations, and outcome has not been systematically investigated in large cohorts. Here, we analyzed CDK6 expression using immunohistochemistry in 203 formalin‐fixed paraffin‐embedded samples of 146 patients and four healthy individuals. We found that 61.5% of all MM specimens express CDK6 at various levels. CDK6 expression increased with the progression of disease with a median of 0% of CDK6‐positive plasma cells in monoclonal gammopathy of undetermined significance (MGUS) ( n = 10) to 30% in newly diagnosed MM ( n = 78) and up to 70% in relapsed cases ( n = 55). The highest median CDK6 was observed in extramedullary myeloma ( n = 12), a highly aggressive manifestation of MM. Longitudinal analyses revealed that CDK6 is significantly increased in lenalidomide‐treated patients but not in those who did not receive lenalidomide. Furthermore, we observed that patients who underwent lenalidomide‐comprising induction therapy had significantly shorter progression‐free survival when their samples were CDK6 positive. These data support that CDK6 protein expression is a marker for aggressive and drug‐resistant disease and describes a potential drug target in MM.
Differential modulation of allergic rhinitis nasal transcriptome by dupilumab and allergy immunotherapy
Authors: Matthew F. Wipperman, Kaitlyn M. Gayvert, Amanda Atanasio, Claire Q. Wang, Jonathan Corren, Angelica Covarrubias, Ian Setliff, Erica Chio, Elizabeth Laws, Kelley Wolfe, Sivan Harel, Jennifer Maloney, Gary Herman, Jamie M. Orengo, Wei Keat Lim, Sara C. Hamon, Jennifer D. Hamilton, Meagan P. O'Brien
Journal: Allergy (2024)

Abstract Background Nasal epithelial cells are important regulators of barrier function and immune signaling; however, in allergic rhinitis (AR) these functions can be disrupted by inflammatory mediators. We aimed to better discern AR disease mechanisms using transcriptome data from nasal brushing samples from individuals with and without AR. Methods Data were drawn from a feasibility study of individuals with and without AR to Timothy grass and from a clinical trial evaluating 16 weeks of treatment with the following: dupilumab, a monoclonal antibody that binds interleukin (IL)‐4Rα and inhibits type 2 inflammation by blocking signaling of both IL‐4/IL‐13; subcutaneous immunotherapy with Timothy grass (SCIT), which inhibits allergic responses through pleiotropic effects; SCIT + dupilumab; or placebo. Using nasal brushing samples from these studies, we defined distinct gene signatures in nasal tissue of AR disease and after nasal allergen challenge (NAC) and assessed how these signatures were modulated by study drug(s). Results Treatment with dupilumab (normalized enrichment score [NES] = −1.73, p = .002) or SCIT + dupilumab (NES = −2.55, p &lt; .001), but not SCIT alone (NES = +1.16, p = .107), significantly repressed the AR disease signature. Dupilumab (NES = −2.55, p &lt; .001), SCIT (NES = −2.99, p &lt; .001), and SCIT + dupilumab (NES = −3.15, p &lt; .001) all repressed the NAC gene signature. Conclusion These results demonstrate type 2 inflammation is an important contributor to the pathophysiology of AR disease and that inhibition of the type 2 pathway with dupilumab may normalize nasal tissue gene expression.
Explaining Health Inequities — The Enduring Legacy of Historical Biases
Authors: David S Jones, Evelynn Hammonds, Joseph P Gone, David Williams
Journal: New England Journal of Medicine (2024)

W hen the Journal was launched in 1812, claims had circu- lated for centuries about differences in anatomy, physiology, and disease susceptibility between different human populations. 1Physicians' persistent belief that these differences are innate has long drawn attention away from other possible causes of health inequities.As the Journal explores its history and acknowledges its role in voicing and perpetuating racism and discrimination, it must examine how it grappled with the problem of difference.The scope of the potential reckoning is vast.In early decades, the Journal published most often about differences among Europeans, Africans, and Indigenous Americans.Similar discourses would emerge about differences between White people (a term once reserved for people of northwestern European ancestry) and Jewish people, Irish people, Mexicans, or Asians, and between men and women. 2-5Over the centuries, health inequities have been described in every major cause of human disease, from smallpox in the 17th century to cancer and heart disease today. 6t's impossible to explore all aspects of this issue in a short review, so we attempt an illustrative analysis, building on previous articles in this series by focusing on Journal articles about Black and Indigenous Americans.Another article will address sex and gender
A Comprehensive Approach to the Thyroid Bethesda Category III (AUS) in the Transition Zone Between 2nd Edition and 3rd Edition of The Bethesda System for Reporting Thyroid Cytopathology: Subcategorization, Nuclear Scoring, and More
Authors: Merve Bagıs, Nuray Can, Necdet Sut, Ebru Tastekin, Ezgi Genc Erdogan, Buket Yilmaz Bulbul, Yavuz Atakan Sezer, Osman Kula, Elif Mercan Demirtas, Inci Usta
Journal: Endocrine Pathology (2024)

Abstract Significant interobserver variabilities exist for Bethesda category III: atypia of undetermined significance (AUS) of The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC). Thus, subcategorization of AUS including AUS “nuclear” and AUS “other” is proposed in the recent 3rd edition of TBSRTC. This study investigated the impact of the nuclear features/architectural features/nuclear score (NS) (3-tiered)/subcategories and subgroups on risk of malignancy (ROM) in thyroid fine-needle aspirations (FNA). 6940 FNAs were evaluated. 1224 (17.6%) cases diagnosed as AUS were reviewed, and 240 patients (initial FNAs of 260 nodules and 240 thyroidectomies) were included. Subcategories and subgroups were defined according to TBSRTC 2nd and 3rd editions. Histological diagnostic groups included nonneoplastic disease, benign neoplasm, low-risk neoplasm, and malignant neoplasm. Overall, ROM was 30.7%. ROM was significantly higher in FNAs with nuclear overlapping (35.5%), nuclear molding (56.9%), irregular contours (42.1%), nuclear grooves (74.1%), chromatin clearing (49.4%), and chromatin margination (57.7%), and these features were independent significant predictors for malignancy. FNAs with NS3 had significantly higher ROM (64.2%). Three-dimensional groups were significantly more frequent in malignant neoplasms (35.7%). ROM was significantly higher in AUS-nuclear subcategory (48.2%) and in AUS-nuclear and architectural subcategory (38.3%). The highest ROM was detected in AUS-nuclear1 subgroup (65.2%). ROM was significantly higher in the group including AUS-nuclear and AUS-nuclear and architectural subcategories, namely “high-risk group” than the group including other subcategories, namely “low-risk group” (42.0%vs 13.9%). In conclusion, subcategorization may not be the end point, and nuclear scoring and evaluation of architectural patterns according to strict criteria may provide data for remodeling of TBSRTC categories.
Convergence of nanomedicine and neutrophils for drug delivery
Authors: Sichen Yuan, Quanyin Hu
Journal: Bioactive Materials (2024)

Neutrophils have recently emerged as promising carriers for drug delivery due to their unique properties including rapid response toward inflammation, chemotaxis, and transmigration. When integrated with nanotechnology that has enormous advantages in improving treatment efficacy and reducing side effects, neutrophil-based nano-drug delivery systems have expanded the repertoire of nanoparticles employed in precise therapeutic interventions by either coating nanoparticles with their membranes, loading nanoparticles inside living cells, or engineering chimeric antigen receptor (CAR)-neutrophils. These neutrophil-inspired therapies have shown superior biocompatibility, targeting ability, and therapeutic robustness. In this review, we summarized the benefits of combining neutrophils and nanotechnologies, the design principles and underlying mechanisms, and various applications in disease treatments. The challenges and prospects for neutrophil-based drug delivery systems were also discussed.
Stem Cells Expansion Vector via Bioadhesive Porous Microspheres for Accelerating Articular Cartilage Regeneration (Adv. Healthcare Mater. 3/2024)
Authors: Lang Bai, Qibin Han, Zeyu Han, Xiaoyu Zhang, Jingwen Zhao, Huitong Ruan, Junliang Wang, Feng Lin, Wenguo Cui, Xing Yang, Yuefeng Hao
Journal: Advanced Healthcare Materials (2024)

Bioadhesive Porous Microsphere In article 2302327, Feng Lin, Wenguo Cui, Xing Yang, Yuefeng Hao and co-workers report a bioadhesive porous microsphere named PLGA-CS@KGN. The microspheres hold high cell-loading ability, adapt to the migration and expansion of cells, and promote MSCs to express markers associated with cartilage repair. It can be used as a potential stem cell carrier for enhancing stem cell therapy in osteoarthritis treatment.
Electroconductive Collagen‐Carbon Nanodots Nanocomposite Elicits Neurite Outgrowth, Supports Neurogenic Differentiation and Accelerates Electrophysiological Maturation of Neural Progenitor Spheroids (Adv. Healthcare Mater. 3/2024)
Authors: David J. Lomboni, Alp Ozgun, Tayline V. de Medeiros, William Staines, Rafik Naccache, John Woulfe, Fabio Variola
Journal: Advanced Healthcare Materials (2024)

Neural Progenitor Spheroids In article 2301894, Fabio Variola and co-workers show a novel electroconductive nanocomposite consisting of a collagen matrix decorated with carbon nanodots supports neurite outgrowth, directs neurogenesis without exogenous factors and elicits the electrophysiological maturation of neural progenitor spheroids.
Spatial Transcriptomics in a Case of Follicular Thyroid Carcinoma Reveals Clone-Specific Dysregulation of Genes Regulating Extracellular Matrix in the Invading Front
Authors: Vincenzo Condello, Johan O. Paulsson, Jan Zedenius, Anders Näsman, C. Christofer Juhlin
Journal: Endocrine Pathology (2024)

Abstract Follicular thyroid carcinoma (FTC) is recognized by its ability to invade the tumor capsule and blood vessels, although the exact molecular signals orchestrating this phenotype remain elusive. In this study, the spatial transcriptional landscape of an FTC is detailed with comparisons between the invasive front and histologically indolent central core tumor areas. The Visium spatial gene expression platform allowed us to interrogate and visualize the whole transcriptome in 2D across formalin-fixated paraffin-embedded (FFPE) tissue sections. Four different 6 × 6 mm areas of an FTC were scrutinized, including regions with capsular and vascular invasion, capsule-near area without invasion, and a central core area of the tumor. Following successful capturing and sequencing, several expressional clusters were identified with regional variation. Most notably, invasive tumor cell clusters were significantly over-expressing genes associated with pathways interacting with the extracellular matrix (ECM) remodeling and epithelial-to-mesenchymal transition (EMT). Subsets of these genes ( POSTN and DPYSL3 ) were additionally validated using immunohistochemistry in an independent cohort of follicular thyroid tumors showing a clear gradient pattern from the core to the periphery of the tumor. Moreover, the reconstruction of the evolutionary tree identified the invasive clones as late events in follicular thyroid tumorigenesis. To our knowledge, this is one of the first 2D global transcriptional mappings of FTC using this platform to date. Invasive FTC clones develop in a stepwise fashion and display significant dysregulation of genes associated with the ECM and EMT — thus highlighting important molecular crosstalk for further investigations.
Controllable Adaptive Molybdate‐Oligosaccharide Nanoparticles Regulate M2 Macrophage Mitochondrial Function and Promote Angiogenesis via PI3K/HIF‐1α/VEGF Pathway to Accelerate Diabetic Wound Healing (Adv. Healthcare Mater. 3/2024)
Authors: Xiuhong Huang, Liqin Zheng, Yueshan Zhou, Shaonan Hu, Wancheng Ning, Simin Li, Ziling Lin, Shaohong Huang
Journal: Advanced Healthcare Materials (2024)

Molybdate-Oligosaccharide Nanosystem The powerful molybdate-oligosaccharide nanosystem (CMO) exhibit pH-responsive release Mo2+ and oligosaccharide to enhance mitochondrial function and facilitate the transition of macrophages from M1 to M2 phenotype. In article 2302256, Xiuhong Huang, Shaohong Huang, and co-workers show that within an anti-inflammatory microenvironment, CMO promote angiogenesis through activation of the PI3K/HIF-1α/VEGF pathway. Such a pH-responsive nanosystem with immunomodulatory properties and angiogenesis- and mitochondrial-promotion capacity presents a promising potential for diabetic wounds repair.
Electrically Controlled Vasodilator Delivery from PEDOT/Silica Nanoparticle Modulates Vessel Diameter in Mouse Brain (Adv. Healthcare Mater. 3/2024)
Authors: Kevin M. Woeppel, Daniela D. Krahe, Elaine M. Robbins, Alberto L. Vazquez, Xinyan Tracy Cui
Journal: Advanced Healthcare Materials (2024)

Drug Delivery In article 2301221, Kevin M. Woeppel and co-workers demonstrate electrically controlled vessel dilator delivery from carbon fiber electrodes coated with a conductive polymer poly(3,4-ethylenedioxythioph ene) and sulfonated silica nanoparticle composite (PEDOT/SNP). Using 2-photon microscopy, they show the electrode coating release the vessel dilator sodium nitroprusside upon electrical triggers and controllably dilate the vasculature surrounding the electrode implanted in the mouse cortex.
Integrating Melt Electrowriting and Fused Deposition Modeling to Fabricate Hybrid Scaffolds Supportive of Accelerated Bone Regeneration (Adv. Healthcare Mater. 3/2024)
Authors: Kian F. Eichholz, Pierluca Pitacco, Ross Burdis, Farhad Chariyev‐Prinz, Xavier Barceló, Brooke Tornifoglio, Ryan Paetzold, Orquidea Garcia, Daniel J Kelly
Journal: Advanced Healthcare Materials (2024)

Bone Healing Applications The cover of article 2302057 by Daniel J Kelly and co-workers shows a false colored scanning electron microscope (SEM) image, showing a hybrid additively manufactured scaffold for bone healing applications, which utilizes fused deposition modelling (FDM, colored blue) to fabricate the scaffold shell, and melt electrowriting (MEW, colored yellow) to fabricate the core. This novel hybrid manufacturing approach has been shown to significantly enhance healing outcomes in large bone defects.
Oxygenated Scaffolds for Pancreatic Endocrine Differentiation from Induced Pluripotent Stem Cells (Adv. Healthcare Mater. 3/2024)
Authors: Hui Huang, Soujanya S. Karanth, Ya Guan, Sebastian Freeman, Ryan Soron, David S. Godovich, Jianjun Guan, Kaiming Ye, Sha Jin
Journal: Advanced Healthcare Materials (2024)

Pluripotent Stem Cells In article 2302275, Sha Jin and co-workers establish an in-situ oxygenated scaffold culture platform to offer a better oxygen supply during differentiation of human induced pluripotent stem cells to endocrine islet cells. The image exhibits the interaction between induced pluripotent stem cells and oxygenated collagen fibers inside the oxygenation-enabled scaffold material.
Laser‐Assisted Structuring of Graphene Films with Biocompatible Liquid Crystal Polymer for Skin/Brain‐Interfaced Electrodes (Adv. Healthcare Mater. 3/2024)
Authors: Rowoon Park, Dong Hyeon Lee, Chin Su Koh, Young Woo Kwon, Seon Yeong Chae, Chang‐Seok Kim, Hyun Ho Jung, Joonsoo Jeong, Suck Won Hong
Journal: Advanced Healthcare Materials (2024)

Tissue-Interfaced Bioelectrodes In article 2301753, Suck Won Hong, Joonsoo Jeong, Hyun Ho Jung, and co-workers report highly conductive graphene films, engraved on a liquid crystal polymer by fully utilizing the laser scribing technique. The presented approaches involve the rapid generation of graphene films in micropatterned arrays and large-scale transfer printing processes on flexible substrates. These techniques allow the preparation of kirigami-structured stretchable and flexible bioelectrodes designed for on-skin electropads and brain-interfaced neural probes, which can be independently connected to healthcare hardware platforms.
Exosomes derived from pulmonary metastatic sites enhance osteosarcoma lung metastasis by transferring the miR-194/215 cluster targeting MARCKS
Authors: Pei Yu, Yubao Han, Lulu Meng, Yanyuan Tian, Zhiwei Jin, Jun Luo, Chao Han, Wenjun Xu, Lingyi Kong, Chao Zhang
Journal: Acta Pharmaceutica Sinica B (2024)

Osteosarcoma, a prevalent primary malignant bone tumor, often presents with lung metastases, severely impacting patient survival rates. Extracellular vesicles, particularly exosomes, play a pivotal role in the formation and progression of osteosarcoma-related pulmonary lesions. However, the communication between primary osteosarcoma and exosome-mediated pulmonary lesions remains obscure, with the potential impact of pulmonary metastatic foci on osteosarcoma progression largely unknown. This study unveils an innovative mechanism by which exosomes originating from osteosarcoma pulmonary metastatic sites transport the miR-194/215 cluster to the primary tumor site. This transportation enhances lung metastatic capability by downregulating myristoylated alanine-rich C-kinase substrate (MARCKS) expression. Addressing this phenomenon, in this study we employ cationic bovine serum albumin (CBSA) to form nanoparticles (CBSA-anta-194/215) via electrostatic interaction with antagomir-miR-194/215. These nanoparticles are loaded into nucleic acid-depleted exosomal membrane vesicles (anta-194/215@Exo) targeting osteosarcoma lung metastatic sites. Intervention with bioengineered exosome mimetics (anta-194/215@Exo) not only impedes osteosarcoma progression but also significantly prolongs the lifespan of tumor-bearing mice. These findings suggest that pulmonary metastatic foci-derived exosomes initiate primary osteosarcoma lung metastasis by transferring the miR-194/215 cluster targeting MARCKS, making the miR-194/215 cluster a promising therapeutic target for inhibiting the progression of patients with osteosarcoma lung metastases.