tbuckley/cabot-search huggingface dataset:
Co-delivery of retinoic acid and miRNA by functional Au nanoparticles for improved survival and CT imaging tracking of MSCs in pulmonary fibrosis therapy
Authors: Xiaodi Li, Shengnan Cheng, Chenggong Yu, Yuxuan Li, Xiaoling Cao, Yuhan Wang, Zhijun Zhang, Jie Huang
Journal: Asian Journal of Pharmaceutical Sciences (2024)

Mesenchymal stem cells (MSCs) have emerged as promising candidates for idiopathic pulmonary fibrosis (IPF) therapy. Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are of great significance for improving the efficacy of MSC-based IPF treatment. Therefore, dual-functional Au-based nanoparticles (Au@PEG@PEI@TAT NPs, AuPPT) were fabricated by sequential modification of cationic polymer polyetherimide (PEI), polyethylene glycol (PEG), and transactivator of transcription (TAT) penetration peptide on AuNPs, to co-deliver retinoic acid (RA) and microRNA (miRNA) for simultaneously enhancing MSC survive and real-time imaging tracking of MSCs during IPF treatment. AuPPT NPs, with good drug loading and cellular uptake abilities, could efficiently deliver miRNA and RA to protect MSCs from reactive oxygen species and reduce their expression of apoptosis executive protein Caspase 3, thus prolonging the survival time of MSC after transplantation. In the meantime, the intracellular accumulation of AuPPT NPs enhanced the computed tomography imaging contrast of transplanted MSCs, allowing them to be visually tracked
Cutaneotropic polyomavirus abundance in the nonlesional skin of keratinocyte carcinoma patients: A cross-sectional study
Authors: Eray Yihui Zhou, Yun Xia, Tomonori Oka, Hiroshi Higuchi, Tatsuya Hasegawa, Jong Ho Park, Emanuela Marchese, Valeria S Oliver-García, Marjan Azin, Danielle N Conrad, Sabrina S Smith, Victor A Neel, Shadmehr Demehri
Journal: Journal of the American Academy of Dermatology (2024)
Navigating the Intersection: Diabetes and Alzheimer's Intertwined Relationship
Authors: Mayur B Kale, Harshavardhan M Bhondge, Nitu L Wankhede, Prajwali V Shende, Rushikesh P Thanekaer, Manish M Aglawe, Sandip R Rahangdale, Brijesh G Taksande, Sunil B Pandit, Aman B Upaganlawar, Milind J Umekar, Spandana Rajendra Kopalli, Sushruta Koppula
Journal: Ageing Research Reviews (2024)
PARPi, BRCA, and gaps: controversies and future research
Authors: Diego Dibitetto, Carmen A. Widmer, Sven Rottenberg
Journal: Trends in cancer (2024)

In recent years, various poly(ADP-ribose) polymerase (PARP) inhibitors (PARPis) have been approved for the treatment of several cancers to target the vulnerability of homologous recombination (HR) deficiency (e.g., due to BRCA1/2 dysfunction). In this review we analyze the ongoing debates and recent breakthroughs in the use of PARPis for BRCA1/2-deficient cancers, juxtaposing the 'double-strand break (DSB)' and 'single-stranded DNA (ssDNA) gap' models of synthetic lethality induced by PARPis. We spotlight the complexity of this interaction, highlighting emerging research on the role of DNA polymerase theta (POLθ) and ssDNA gaps in shaping therapy responses. We scrutinize the clinical ramifications of these findings, especially concerning PARPi efficacy and resistance mechanisms, underscoring the heterogeneity of BRCA-mutated tumors and the urgent need for advanced research to bridge the gap between laboratory models and patient outcomes.
Treatment of obstructive sleep apnea syndrome (OSAS) with mandibular advancement devices—A statement of the Portuguese Society of Pulmonology, the Portuguese Society of Stomatology and Dental Medicine, the Portuguese Dental Association, and the Portuguese Society of Temporomandibular Disorders, Orofacial Pain and Sleep
Authors: Susana Sousa, Sílvia Correia, André Mariz de Almeida, Gabriela Videira, Ricardo Dias, Susana Falardo Ramos, Júlio Fonseca
Journal: Pulmonology (2024)

With the purpose of establishing a consensus around clinical orientations for professionals involved in managing patients with sleep breathing disorders (SBD), an interdisciplinary group of scientific societies involved in this field discussed and reviewed all the published international guidelines from the American Dental Association, American Academy of Sleep Medicine, American Academy of Dental Sleep Medicine and the European counterparts. Treatment of SBD is multidisciplinary and should be made in concert with the patient, the sleep physician, and the qualified dentist to solve the individual, social, and economic burden of the disease,. This consensus document represents the current thinking of a team of Portuguese experts on managing patients with SBD based on the available evidence.
HIV-1 infection kinetics, drug resistance, and long-term safety of pre-exposure prophylaxis with emtricitabine plus tenofovir alafenamide (DISCOVER): week 144 open-label extension of a randomised, controlled, phase 3 trial
Authors: David A Wohl, Christoph D Spinner, Jason Flamm, C Bradley Hare, Susanne Doblecki-Lewis, Peter J Ruane, Jean-Michel Molina, Anthony Mills, Cynthia Brinson, Moti Ramgopal, Amanda Clarke, Gordon Crofoot, Claudia Martorell, Christoph Carter, Stephanie Cox, J Carlo Hojilla, Yongwu Shao, Moupali Das, Alexander Kintu, Jared M Baeten, Robert M Grant, Karam Mounzer, Kenneth Mayer
Journal: The Lancet HIV (2024)
A gain-of-function variant in <i>SREBF1</i> causes generalized skin hyperpigmentation with congenital cataracts
Authors: Huijun Wang, Yuan Wu, Jennifer A Bassetti, Zhaoyang Wang, Vikash S Oza, Sneha A Rangu, Bobbi McGivern, Sha Peng, Lina Liang, Shimiao Huang, Zhuoqing Gong, Zigang Xu, Zhimiao Lin
Journal: British Journal of Dermatology (2024)

Abstract Background Lipid metabolism has essential roles in skin barrier formation and the regulation of skin inflammation. Lipid homeostasis regulates skin melanogenesis, although the underlying mechanism remains largely unknown. Sterol regulatory element binding protein 1 (SREBP-1) is a key transcription factor essential for cellular lipid metabolism. Loss-of-function variants in SREBF1 are responsible for autosomal-dominant ichthyosis follicularis, alopecia and photophobia syndrome, emphasizing the significance of lipid homeostasis in skin keratinization. Objectives To identify the genetic basis of a new entity featuring diffuse skin hyperpigmentation with congenital cataracts, and to unravel the underlying mechanism for the pathogenesis of the SREBF1 variant. Methods Whole-exome sequencing was performed to identify underlying genetic variants. Quantitative polymerase chain reaction, Western blot and immunofluorescence staining were used to assess the expression and the subcellular localization of the SREBF1 variant. The transcriptional activity of mutant SREBP-1 was determined by a luciferase reporter assay. A transgenic zebrafish model was constructed. Results Two unrelated patients presented with generalized skin hyperpigmentation with skin xerosis, congenital cataracts and extracutaneous symptoms. We identified a de novo nonsense variant c.1289C&amp;gt;A (p.Ser430*) in SREBF1 in both patients. The variant encoded a truncated protein that showed preferential nucleus localization, in contrast to wild-type SREBP-1 which – in sterol-sufficient conditions – is mainly localized in the cytoplasm. The luciferase reporter assay revealed that the p.Ser430* mutant exhibited enhanced transcriptional activity. Cultured patient primary melanocytes showed increased melanin synthesis vs. those from healthy controls. At 35 days postfertilization, the p.Ser430* transgenic zebrafish model exhibited more black spots, along with upregulated expression of melanogenic genes. Conclusions We demonstrated that a gain-of-function variant of SREBF1 causes a previously undescribed disorder characterized by generalized skin hyperpigmentation and congenital cataracts. Our study reveals the involvement of SREBP-1 in melanogenesis and lens development, and paves the way for the development of novel therapeutic targets for skin dyspigmentation or cataracts.
Dupilumab-associated (hyper)eosinophilia in patients with atopic dermatitis: a single-centre cohort study of the TREAT NL (TREatment of ATopic eczema, the Netherlands) registry
Authors: Angelo Li, Annelie H Musters, Ariënna Hyseni, Louise A A Gerbens, Phyllis I Spuls
Journal: British Journal of Dermatology (2024)

This study investigated the incidence and progression of (hyper)eosinophilia in patients with atopic dermatitis (AD) treated with dupilumab, using data from the TREAT NL registry cohort. In total, 38.5% of patients had (hyper)eosinophilia at baseline and/or during dupilumab treatment. (Hyper)eosinophilia was present at baseline in 43 (55.8%) patients and developed in 34 (44.2%) patients during treatment. Eosinophilia was transient, peaking at 6 months and normalizing by 18–24 months. One patient discontinued dupilumab due to persistent hypereosinophilia, but generally, eosinophilia did not affect treatment outcomes. In conclusion, dupilumab is found viable for patients with AD regardless of baseline eosinophilia, with temporary increases in eosinophil counts that are often not clinically significant.
Correlative Analysis of ATM, RB1, ERCC2, and FANCC Mutations and Pathologic Complete Response After Neoadjuvant Chemotherapy in Patients with Muscle-invasive Bladder Cancer: Results from the SWOG S1314 Trial
Authors: Elizabeth R Plimack, Catherine Tangen, Melissa Plets, Rutika Kokate, Joanne Xiu, Chadi Nabhan, Eric A Ross, Erin Grundy, Woonyoung Choi, Colin P N Dinney, I-Ling C Lee, Megan Fong, M Scott Lucia, Siamak Daneshmand, Dan Theodorescu, Amir Goldkorn, Seth P Lerner, Thomas W Flaig, David J McConkey
Journal: European Urology (2024)
ROS-responsive charge reversal mesoporous silica nanoparticles as promising drug delivery system for neovascular retinal diseases
Authors: Ahmed M. Elbedwehy, Jun Wu, Hee-Kyung Na, Ahruem Baek, Haejin Jung, Ik Hwan Kwon, Sang Won Lee, Jeong Hun Kim, Tae Geol Lee
Journal: Journal of Controlled Release (2024)

Intravitreal injection of biodegradable implant drug carriers shows promise in reducing the injection frequency for neovascular retinal diseases. However, current intravitreal ocular devices have limitations in adjusting drug release rates for individual patients, thereby affecting treatment effectiveness. Accordingly, we developed mesoporous silica nanoparticles (MSNs) featuring a surface that reverse its charge in response to reactive oxygen species (ROS) for efficient delivery of humanin peptide (HN) to retinal epithelial cells (ARPE-19). The MSN core, designed with a pore size of 2.8 nm, ensures a high HN loading capacity 64.4% (w/w). We fine-tuned the external surface of the MSNs by incorporating 20% Acetyl-L-arginine (Ar) to create a partial positive charge, while 80% conjugated thioketal (TK) methoxy polyethylene glycol (mPEG) act as ROS gatekeeper. Ex vivo experiments using bovine eyes revealed the immobilization of Ar-MSNs-TK-PEG (mean zeta potential: 2 mV) in the negatively charged vitreous. However, oxidative stress reversed the surface charge to -25 mV by mPEG loss, facilitating the diffusion of the nanoparticles impeded with HN. In vitro studies showed that ARPE-19 cells effectively internalize HN-loaded Ar-MSNs-TK, subsequently releasing the peptide, which offered protection against oxidative stress-induced apoptosis, as evidenced by reduced TUNEL and caspase3 activation. The inhibition of retinal neovascularization was further validated in an in vivo oxygen-induced retinopathy (OIR) mouse model.
Comparison of left ventricular mass and wall thickness between cardiac computed tomography angiography and cardiac magnetic resonance imaging using machine learning algorithms
Authors: Finn Y van Driest, Rob J van der Geest, Sharif K Omara, Alexander Broersen, Jouke Dijkstra, J Wouter Jukema, Arthur J H A Scholte
Journal:  (2024)

Abstract Aims Cardiac magnetic resonance imaging (MRI) is the gold standard in the assessment of left ventricle (LV) mass and wall thickness. In recent years, cardiac computed tomography angiography (CCTA) has gained widespread usage as an imaging modality. Despite this, limited previous investigations have specifically addressed the potential of CCTA as an alternative modality for quantitative LV assessment. The aim of this study was to compare CCTA derived LV mass and wall thickness with cardiac MRI utilizing machine learning algorithms. Methods and results Fifty-seven participants who underwent both CCTA and cardiac MRI were identified. LV mass and wall thickness was calculated using LV contours which were automatically placed using in-house developed machine learning models. Pearson’s correlation coefficients were calculated along with Bland–Altman plots to assess the agreement between the LV mass and wall thickness per region on CCTA and cardiac MRI. Inter-observer correlations were tested using Pearson’s correlation coefficient. Average LV mass and wall thickness for CCTA and cardiac MRI were 127 g, 128 g, 7, and 8 mm, respectively. Bland–Altman plots demonstrated mean differences and corresponding 95% limits of agreement of −1.26 (25.06; −27.58) and −0.57 (1.78; −2.92), for LV mass and average LV wall thickness, respectively. Mean differences and corresponding 95% limits of agreement for wall thickness per region were −0.75 (1.34; −2.83), −0.58 (2.14; −3.30), and −0.29 (3.21; −3.79) for the basal, mid, and apical regions, respectively. Inter-observer correlations were excellent. Conclusion Quantitative assessment of LV mass and wall thickness on CCTA using machine learning algorithms seems feasible and shows good agreement with cardiac MRI.
The burden of cirrhosis mortality by county, race, and ethnicity in the USA, 2000–19: a systematic analysis of health disparities
Authors: Hasan Nassereldine, Kelly Compton, Zhuochen Li, Mathew M Baumann, Yekaterina O Kelly, Wichada La Motte-Kerr, Farah Daoud, Erik J Rodriquez, George A Mensah, Anna M Nápoles, Eliseo J Pérez-Stable, Christopher J L Murray, Ali H Mokdad, Laura Dwyer-Lindgren
Journal: The Lancet Public Health (2024)
Doughnut-shaped endoscopic submucosal dissection for circumferential ileocecal valve adenoma
Authors: Yugo Suzuki, Kosuke Nomura, Hanako Inoue, Daisuke Kikuchi, Akira Matsui, Shu Hoteya
Journal: Endoscopy (2024)

Endoscopic submucosal dissection (ESD) has not become established as a standard technique for treatment of ileocecal valve (ICV) lesions, and its efficacy has been reported as limited because of its technical difficulty and the time required [1] [2] [3]. Regarding circumferential lesions in particular, there have been few reports of treatment with ESD [4].
Alternative endoscopic ultrasound-guided choledochogastrostomy for anatomical inaccessibility by usual biliary drainage methods
Authors: Hiroaki Tsuji, Ryota Sagami, Takao Sato, Hidefumi Nishikiori, Yasuhisa Hiroshima, Kazuhiro Mizukami, Kazunari Murakami
Journal: Endoscopy (2024)

A 92-year-old man was admitted with jaundice and cholangitis. Computed tomography (CT) and magnetic resonance cholangiopancreatography (MRCP) revealed intrahepatic/middle bile duct dilatation, with a pancreatic head lesion suspicious for carcinoma ([Fig. 1] a, c); the intraperitoneal organs were drawn into the right thoracic cavity due to Chilaiditi syndrome ([Fig. 1] b). Despite wire-guided insertion, endoscopic retrograde cholangiography (ERC) could not reach the papilla of Vater owing to anatomical abnormalities ([Fig. 2]). Endoscopic ultrasound-guided hepaticogastrostomy failed because of invisibility of the left intrahepatic duct. The common bile duct (CBD), which in normal anatomy is not clearly visualized from the gastric tract, could be visualized from the antrum. Endoscopic ultrasound-guided choledochogastrostomy (EUS-CGS) was ultimately performed ([Video 1]).
Dual-sided closure of an iatrogenic gastrocolonic fistula with over-the-scope clips
Authors: Sunil Gupta, Roxana Chis, Jeffrey D. Mosko, Christopher Teshima
Journal: Endoscopy (2024)

An 86-year-old man with walled-off pancreatic necrosis (WON) was initially managed with a percutaneous drain. He was referred to our service because of a persistent collection. After fluid had been instilled via the drain to expand the collapsed collection, endoscopic ultrasound (EUS)-guided cystogastrostomy was performed with a lumen-apposing metal stent (LAMS; 15-mm wide × 10 mm in length). A computed tomography (CT) scan the following week revealed a gastrocolostomy due to stent misdeployment. As the patient was clinically stable, we opted to wait 4 weeks to facilitate tract maturation prior to LAMS removal and definitive closure [1].
Endoscopic submucosal dissection for resection of giant adenoid cystic carcinoma of the esophagus
Authors: Yu Xia, Jian Li, Guanzhang Wen, Qiqi Zhang, Jingjing Yao, Zhiqiang Zhang
Journal: Endoscopy (2024)

A 53-year-old man presented with progressive dysphagia and retrosternal pain. A gastroscope revealed a giant pronounced protrusion lesion with a thick pedicle in the lower esophagus measuring approximately 60 × 25 mm. Further refinement via computed tomography (CT) indicated an occupying lesion in the distal esophagus with no evidence of metastatic disease present; this led to the suggestion of performing an endoscopic resection
A novel gastric defect closure method using lines and reopenable clips with the through-the-scope tying technique
Authors: Junki Toyoda, Tatsuma Nomura, Takanobu Mitani, Yuto Ikadai, Sase Tomohiro, Tomonori Saito, Katsumi Mukai
Journal: Endoscopy (2024)

Gastric endoscopic submucosal dissection (ESD) defect closure is a difficult procedure in terms of achieving complete closure. Recently, a useful method for closing defects using a line and needle was reported [1]; however, the endoscope must be withdrawn for ligation of the lines. Therefore, we have devised a new closure method, the "through-the-scope tying technique" (TTST), in which the lines are tied by hand outside of the body and can be ligated through the endoscope accessory channel.
Double nylon loop-based inner traction technique promoting endoscopic submucosal dissection of a giant pedunculated adenoma in the ascending colon
Authors: Fu-qiang Liu, Xiao Hu, Yun-chao Yang, Xiangrong Zhou, Wenjuan Ding, Zhi-qiang Du, Wei-hui Liu
Journal: Endoscopy (2024)

Endoscopic submucosal dissection (ESD) is feasible for the removal of giant pedunculated polyps with thick stalks when conventional snare resection is difficult [1] [2]. Although pretreatment of the stalks can prevent bleeding in thick-pedunculated polyps, some cases simultaneously comprising massive polyp heads often cause restricted operability and poor visibility, thereby increasing operative challenges [3] [4] [5]. Herein, we describe a novel inner traction technique to facilitate ESD of a large thick-pedunculated polyp by securely ligating and effectively pulling the stalk with double nylon loops ([Video 1]).
Clip-anchored floss traction-assisted endoscopic submucosal dissection combined with endoscopic tunneling in treatment of long circumferential early esophageal carcinoma
Authors: Wen-juan Ding, Xiang-rong Zhou, Fu-qiang Liu, Zhi-qiang Du, Wei-hui Liu
Journal: Endoscopy (2024)

During endoscopic submucosal dissection (ESD) for large esophageal lesions, problems like lumen obstruction caused by distal contraction of the detached tissue, narrow submucosal space, and poor exposure of the submucosa often occur [1] [2] [3]. Although tunneling techniques can reportedly treat such lesions, they have not been able to resolve the issue of the dissected tissue clogging the lumen, and dissecting the ridges between the tunnels is challenging [4] [5]. In this study, we used a tunneling technique combined with floss traction to improve the long circumferential ESD of early esophageal cancer ([Video 1]).
Salvage circumferential endoscopic submucosal dissection of multifocal Barrett’s adenocarcinoma at an esophagogastric anastomosis
Authors: Sunil Gupta, Tony He, Jeffrey D. Mosko, Gary May, Christopher Teshima
Journal: Endoscopy (2024)

A 69-year-old man underwent neoadjuvant chemoradiotherapy and Ivor Lewis esophagectomy with proximal gastrectomy for T3N0M0 esophageal adenocarcinoma. After 6 years, a new positron emission tomography (PET)-avid thickening was detected at the esophagogastric anastomosis. Endoscopy revealed a 3-cm circumferential segment of Barrett's mucosa extending from the esophagogastric anastomosis into the remnant esophagus. This contained multiple areas of nodularity with irregular pit and vascular pattern that were concerning for multifocal early Barrett's cancer ([Fig. 1]). As the patient was not a candidate for further surgery or radiotherapy, we proceeded with salvage circumferential endoscopic submucosal dissection (ESD) ([Video 1]).
Endoscopic suturing system is the last chance for rectal bleeding after complicated endoscopic submucosal dissection
Authors: Martina De Siena, Federico Barbaro, Vincenzo Bove, Maria Valeria Matteo, Valerio Pontecorvi, Ivo Boškoski, Cristiano Spada
Journal: Endoscopy (2024)

We report the case of a 54-year-old woman who underwent endoscopic submucosal dissection (ESD) for a 3-cm laterally spreading tumor (LST) of the mid-proximal rectum ([Fig. 1]). The ESD was complicated by intraoperative bleeding from visible vessels of the defect. Use of hemostatic forceps (Coagrasper) and endoclip placement led to resolution of the bleeding ([Fig. 2]).
Prophylactic saline-immersion snare-tip vessel coagulation after colorectal endoscopic resection
Authors: Antonio Capogreco, Roberto de Sire, Davide Massimi, Ludovico Alfarone, Roberta Maselli, Cesare Hassan, Alessandro Repici
Journal: Endoscopy (2024)

Endoscopic mucosal resection (EMR) for large colorectal adenomatous lesions is hampered by a relevant risk of post-EMR delayed bleeding [1] [2]. Patients who have proximal lesions and/or are on antithrombotic treatment are at higher risk for such delayed bleeding [3].
A transanal alpha-loop tube facilitates endoscopic submucosal dissection of a distal rectal tumor
Authors: Yuka Kagaya, Yoshikazu Hayashi, Takaaki Morikawa, Hiroki Hayashi, Hisashi Fukuda, Stefano Kayali, Hironori Yamamoto
Journal: Endoscopy (2024)

Collapsing the lumen with frequent gas aspiration maintains thick submucosal tissue and stabilizes endoscope controllability during endoscopic submucosal dissection (ESD) [1] [2]. We introduced the placement of drainage tubes to drain gas and fluid [3] [4]. Although we proved the usefulness of a Foley catheter as a drainage route from the rectum, its cost was not cheap, at 2000 Japanese yen (JPY). We hypothesized that an nasogastric tube with a looped tip in an alpha shape ([Fig. 1]), at 88 JPY, would instead work as well as the catheter.
Oral versus intravenous empirical antibiotics in children and adolescents with uncomplicated bone and joint infections: a nationwide, randomised, controlled, non-inferiority trial in Denmark
Authors: Allan Bybeck Nielsen, Mette Holm, Morten S Lindhard, Jonathan P Glenthøj, Luise Borch, Ulla Hartling, Lisbeth S Schmidt, Maren J H Rytter, Annett H Rasmussen, Mads Damkjær, Grethe Lemvik, Jens J H Petersen, Mia J Søndergaard, Jesper Thaarup, Kim Kristensen, Lise H Jensen, Lotte H Hansen, Marie C Lawaetz, Martin Gottliebsen, Tanja H Horsager, Tatjana Zaharov, Thomas U Hoffmann, Tobias Nygaard, Ulrik S Justesen, Lone G Stensballe, Nadja H Vissing, Paul Blanche, Kjeld Schmiegelow, Ulrikka Nygaard
Journal: The Lancet Child & Adolescent Health (2024)
Multi-organ immune-related adverse events from immune checkpoint inhibitors and their downstream implications: a retrospective multicohort study
Authors: Guihong Wan, Wenxin Chen, Sara Khattab, Katie Roster, Nga Nguyen, Boshen Yan, Ahmad Rajeh, Jayhyun Seo, Hannah Rashdan, Leyre Zubiri, Matthew J Hadfield, Shadmehr Demehri, Kun-Hsing Yu, William Lotter, Alexander Gusev, Nicole R LeBoeuf, Kerry L Reynolds, Shawn G Kwatra, Yevgeniy R Semenov
Journal: The Lancet Oncology (2024)