tbuckley/cabot-search huggingface dataset:
Economic impact of RSV infections in young children attending primary care: a prospective cohort study in five European countries, 2021 to 2023
Authors: Valérie DV Sankatsing, Sarah F Hak, Joanne G Wildenbeest, Roderick P Venekamp, Mauro Pistello, Caterina Rizzo, Santiago Alfayate-Miguélez, Daan Van Brusselen, Marta Carballal-Mariño, Uy Hoang, Rolf Kramer, Simon de Lusignan, Oliver Martyn, Marc Raes, Adam Meijer, Jojanneke van Summeren
Journal: Eurosurveillance (2025)

Background Data on economic costs of respiratory syncytial virus (RSV) infections among children in primary care are scarce, although most RSV-infections are managed in this setting. Aim To estimate outpatient costs for RSV-positive children aged &lt; 5 years. Methods In the RSV ComNet prospective cohort, children &lt; 5 years with acute respiratory infection were recruited for RSV testing through primary care physicians in Belgium, Italy, the Netherlands, Spain and the United Kingdom (UK) during RSV seasons 2020/21 (UK only), 2021/22 and 2022/23. Outpatient healthcare utilisation and parental work absence were assessed over 30 days through parental questionnaires. Average costs per RSV episode were calculated from outpatient healthcare sector and societal perspectives, stratified by country and age. Results We included 3,414 children and 1,124 (33%) tested RSV-positive. Physicians completed reports for 878 episodes, with follow-up questionnaire data for 819 (93%). Outpatient costs ranged from EUR 97 (95% CI: 91–104) in the Netherlands to EUR 300 (95% CI: 287–312) in Spain and were higher for infants than children aged 1–5 years. Societal costs ranged from EUR 454 (95% CI: 418–494) in the UK to EUR 994 (95% CI: 938–1,053) in Belgium. For children aged 1–5 years, societal costs were primarily driven by parental work absence. In infants, the main societal cost driver varied by country, but overall outpatient healthcare costs represented a higher proportion of societal costs vs older children. Conclusion RSV infections in children attending primary care result in substantial economic costs per episode, although differences exist across countries. This study provides essential data to inform cost-effectiveness analyses on novel RSV immunisations.
Germ cell and other tumors in individuals with differences in sex development
Authors: Selma Feldman Witchel, Miguel Reyes‐Múgica
Journal: CA A Cancer Journal for Clinicians (2025)

Abstract Approximately one in 3500 to one in 5100 live‐born infants have atypical external genital development, known as differences in sex development (DSD). In 2005, an expert consensus conference thoroughly reviewed aspects of health care for individuals with DSD. The conference proposed a classification system to help provide individualized evaluations and management. Some types of DSD are associated with germ cell tumors, which comprise a heterogeneous group of neoplasms derived from germline cells. These neoplasms commonly occur in infants, children, adolescents, and young adults. Herein, an overview of DSDs and risks for germ cell tumors is provided.
Klebsiella aerogenes ST117 causing folliculitis in men having sex with men, Belgium, February 2025
Authors: Nicolas Yin, Jonathan Krygier, Célestin Mairesse, Agnès Libois, Sophie Quoilin, Delphine Martiny
Journal: Eurosurveillance (2025)

Klebsiella aerogenes has recently been reported as a causative agent of folliculitis in men who have sex with men (MSM). We present four cases of folliculitis in MSM diagnosed in Brussels, Belgium. Patients were aged between 25 and 50 years, and all were infected by a single multilocus sequence type (ST117) strain. This strain carried the yersiniabactin siderophore genes. Following a preliminary treatment by sulfamethoxazole−trimethoprim for 7−14 days, all patients experienced a recurrence of symptoms, necessitating an extended therapeutic regimen.
From multiple measles genotype D8 introductions in 2024 to sustained B3 local transmission in and around Milan, northern Italy, January to April 2025
Authors: Clara Fappani, Maria Gori, Silvia Bianchi, Lucia Tieghi, Daniela Colzani, Sabrina Senatore, Marino Faccini, Priscilla Pasutto, Luca Imeri, Luigi Vezzosi, Gabriele Del Castillo, Simone Villa, Danilo Cereda, Silvia Gioacchini, Paola Bucci, Raoul Fioravanti, Emilio D’Ugo, Melissa Baggieri, Fabio Magurano, Antonella Amendola
Journal: Eurosurveillance (2025)

An outbreak of measles virus genotype B3 is ongoing in Milan and surrounding areas since February 2025, with 27 cases identified in 32 laboratory-confirmed measles cases. Most cases were locally acquired and young adults. Phylogenetic analysis indicated the presence of a unique lineage closely related to strains circulating in Morocco. The lack of epidemiological links between several affected individuals suggests case numbers are being underestimated. The continuous transmission raises concerns about the potential re-establishment of endemic circulation in northern Italy.
Spread of the novel vancomycin-resistant Enterococcus faecium strain ST1299/vanA from local level in Germany to cross-border level in Austria, 2018 to 2022
Authors: Anca Rath, Bärbel Kieninger, Nilufarbayim Mirzaliyeva, Guido Werner, Jennifer K Bender, Martin A Fischer, Adriana Cabal-Rosel, Werner Ruppitsch, Helena MB Seth-Smith, Adrian Egli, Milo Halabi, Anna Hörtenhuber, Yarub Salaheddin, Wolfgang Prammer, Heidrun Kerschner, Rainer Hartl, Martin Ehrenschwender, Andreas Ambrosch, Jörn Kalinowski, Levin Joe Klages, Christian Rückert-Reed, Tobias Busche, Alexander Kratzer, Aila Caplunik-Pratsch, Anja Eichner, Jürgen Fritsch, Wulf Schneider-Brachert
Journal: Eurosurveillance (2025)

Introduction Vancomycin-resistant Enterococcus faecium (VREfm) isolates of sequence type (ST)1299 were described recently in south-eastern German hospitals and rapidly expanded from local to cross-border level. Aim We describe the spread of the novel VREfm strain ST1299/vanA on a genetic, geographical and temporal level during the first 5 years after its detection. Methods At University Hospital Regensburg (UHoR), routine VREfm surveillance is whole genome sequencing-based (≥ 1 VREfm per van -genotype, patient and year). In this observational cohort study, we analysed one VREfm ST1299 isolate from our database (2016–2022) per patient and year. Isolates were added from the Hospital of the Merciful Brothers Regensburg (MBR), the National Reference Centre for Staphylococci and Enterococci (NRC), and clinical isolates from Austria. Results We identified 635 VREfm ST1299 isolates (100% vanA ), including 504 from Regensburg, and 113 blood cultures. ST1299 isolates were first detected in 2018 simultaneously in Regensburg (n = 2) and southern Bavaria (n = 2), with local (UHoR) and regional numbers increasing rapidly from 2020, shifting to national scale in the same year. Genome data, analysed by cgMLST, showed a predominance of ST1299/CT1903 (315/504 isolates, 62.5%) and ST1299/CT3109 (127/504 isolates, 25.2%) isolates from Regensburg. By 2021, ST1299/CT1903 reached Upper Austria causing hospital outbreaks (n = 5). Phylogeny analysis suggests common ancestors with VREfm ST80, ST18 and ST17. Conclusion Since their emergence in 2018, two highly transmissible subtypes of ST1299/ vanA reached national, then cross-border scale. The observed outbreak tendency may explain the rapid and successful spread and the high clonality in our collection.
Assisted reproductive technology and risk of malignancy among offspring: a meta-analysis
Authors: Linyu Zhang, Gang Tang, Xinrui Sun, Xue Xiao, Fang Ma
Journal: International Journal of Surgery (2025)

Background: With the popularization of assisted reproductive technology (ART), the question of whether ART increases the risk of malignancy in offspring has received increasing attention. Although many studies have explored the relationship between ART use and malignancy in offspring, the results remain controversial. Materials and methods: Two authors used the Embase, Web of Science, PubMed, and the Cochrane Library databases to conduct a systematic search of published studies on the effects of ART on the risk of malignancy in offspring. Odds ratios (OR) and 95% confidence intervals were used for the analysis. Results: Twenty cohort and four case–control studies were included in this review. ART did not increase the risk of overall malignancy in the offspring (OR, 1.04) compared with natural pregnancy (NP). However, the subgroup analysis showed that the offspring in the ART group had a higher risk of leukemia (OR, 1.24), soft tissue tumors (OR, 1.35), hepatic tumors (OR, 2.10), and epithelial tumors and melanoma (OR, 1.50). The risks of lymphoma, retinoblastoma, central nervous system tumors, neuroblastoma, renal tumors, germ cell and gonad tumors, and embryonic tumors did not differ between the ART and NP groups. Subgroup analysis based on ART type showed that in vitro fertilization, intracytoplasmic sperm injection, frozen embryo transfer, and intrauterine insemination or ovulation did not increase the risk of overall malignancy compared to the NP group. Conclusions: ART may not be associated with the risk of overall malignancy in offspring. However, we found that ART may be associated with an increased risk of leukemia, soft tissue tumors, hepatic tumors, epithelial tumors, and melanoma.
Development and application of an intelligent platform for automated recognition of surgical instruments in laparoscopic procedures: a multicenter retrospective study [experimental studies]
Authors: Ran Hu, Ming Tang, Yi Jin, Lelang Xiang, Keyu Li, Bing Peng, Jie Liu, Dian Qin, Long Liang, Yichuan Li, Linxun Liu, Chunrong Wang, Yong Xiong, Peilin Dai, Ang Li, Xin Wang
Journal: International Journal of Surgery (2025)

Background Surgical videos offer rich intraoperative data critical for surgical education, quality control, and skill assessment. However, their increasing volume and complexity render manual review impractical. To address this challenge, this study aims to develop an intelligent platform capable of automatically recognizing and visualizing intraoperative surgical instrument usage, and to evaluate its clinical utility in real-world surgical settings. Materials and Methods Surgical videos from 21 medical centers in China, covering five surgical types, were collected to develop a generalized artificial intelligence (AI) model for automated surgical instrument recognition. The model was deployed on the SurgSmart platform which features clinically oriented functions. A multicenter survey involving 30 surgeons was conducted to assess the clinical value of the platform. Results A total of 1,261 surgical videos were collected, from which 96,324 images were extracted and annotated with 268,828 labels. The developed model achieved a mean Average Precision (mAP) of 80.31% for recognizing 21 surgical instruments. Based on this model, four core functions were implemented on SurgSmart: Rapid Review Mode, Surgical Instrument Report, Surgical Instrument Heatmap, and Surgical Teaching Mode. All participating surgeons reported a high level of satisfaction and acknowledged the clinical relevance of these functionalities. Conclusion A universally applicable surgical instrument recognition model was developed and deployed on SurgSmart to enable the visualization of intraoperative instrument usage, demonstrating promising clinical potential for automated surgical video analysis and enhanced intraoperative data interpretation.
Genotype-Specific Outcomes of Desmosomal Cardiomyopathies
Authors: Valerio Pergola, Alessandro Trancuccio, Deni Kukavica, Andrea Mazzanti, Carlo Napolitano, Gabriele Gaetano Scilabra, Kenneth Steele, Mirella Memmi, Patrick Gambelli, Andrea Sugamiele, Alessia Chiara Latini, Nicola Pisani, Giulio Mazzotta, Raffaella Bloise, Massimo Morini, Maira Marino, Silvia G. Priori
Journal: Circulation (2025)

BACKGROUND: Desmosomal gene variants (DGVs) have been associated with a diverse spectrum of phenotypic manifestations within arrhythmogenic cardiomyopathy, but data on genotype-specific outcomes are lacking. We investigated genotype-specific arrhythmic and heart failure (HF) outcomes in DGV carriers. METHODS: This cohort study included consecutive patients referred for screening for desmosomal genes. Carriers of pathogenic and rare (allele frequency &lt;10 − 4 ) variants of uncertain significance were included. The arrhythmic end point was the occurrence of a life-threatening arrhythmic event, defined as sudden cardiac death, aborted cardiac arrest, or hemodynamically unstable ventricular tachycardia. The end-stage HF outcome was the composite of a fatal HF episode or cardiac transplantation. RESULTS: We included 533 DGV carriers (59% male; median [interquartile range] age, 39 [22–54] years) from 214 families: 503 of 533 had a single DGV (212 [40%] PKP2 , 160 [30%] DSP , 97 [18%] DSG2 , 34 [6%] DSC2 ) and 30 of 533 (6%) double DGVs. Overall, 83 of 533 (16%) experienced a life-threatening arrhythmic event (at age 40 [33–51] years), and 14 of 533 (3%) experienced end-stage HF (at age 57 [50–60] years). Multivariable analysis demonstrated that, compared with nonmissense PKP2 variants, nonmissense DSP variants (hazard ratio [HR], 2.3 [95% CI, 1.3–4.1]; P =0.008), missense variants in hotspot domains in DSP (HR, 2.7 [95% CI, 1.2–6.2]; P =0.010) and PKP2 (HR, 3.6 [95% CI, 2.0–6.5]; P &lt;0.001), male sex (HR, 1.7 [95% CI, 1.1–2.8]; P =0.021), and double DGVs (HR, 3.4 [95% CI, 1.7–6.9]; P &lt;0.001) were associated with a higher risk of a life-threatening arrhythmic event. Nonmissense DSP variants (HR, 5.0 [95% CI, 1.5–18.2]; P =0.009) and double DGVs (HR, 4.7 [95% CI, 1.0–19.3]; P =0.044) were also associated with increased risk of end-stage HF. CONCLUSIONS: Among carriers of DGVs, genotype was associated with arrhythmic and HF outcomes, with type and location of the variant further modulating the natural history.
Long Non-coding RNA NORAD Suppresses Erastin-induced Ferroptosis in Breast Cancer by Upregulating SLC7A11 via Targeting the FUS/NR3C1 Axis-experimental Studies
Authors: Pengfei Shi, Bo Hu, Yongjun Liu, Xun Li, Wenhuan Li, Chunwei Huang, Ming Jiang, Yang Liu
Journal: International Journal of Surgery (2025)

Background: Ferroptosis, an iron-dependent cell death, has been widely reported to impede the progression of various malignancies, including breast cancer (BC). Previous evidence has indicated that high expression of non-coding RNA activated by DNA damage (NORAD) is correlated with poor prognosis in BC patients. Nonetheless, it is unclear whether NORAD plays a role in the ferroptosis of BC cells. Methods: BC cells were treated with the ferroptosis agonist erastin to induce ferroptosis. Western blotting and qRT-PCR were employed to determine protein and RNA levels, respectively. The cell counting kit-8 assay was used to assess cell viability. Mitochondrial morphology, lipid peroxidation, and intracellular Fe 2+ , MDA, and GSH levels were evaluated for ferroptosis. ChIP, luciferase reporter, and RIP assays were conducted to explore the molecular mechanism of NORAD. A xenograft mouse model (BALB/c nude) was established to evaluate NORAD’s effect on ferroptosis of BC in vivo . Results: NORAD displayed a high level in human BC tissues and cells. NORAD overexpression or silencing inhibited or facilitated erastin-induced ferroptosis of BC cells, respectively. NORAD upregulated NR3C1 expression via interaction with FUS, and NR3C1 could transcriptionally upregulate SLC7A11. Overexpressing SLC7A11 could reverse NORAD silencing-mediated promotion of ferroptosis. NORAD upregulation attenuated the antitumor activity of erastin in tumor-bearing mice. Conclusions: NORAD upregulates SLC7A11 via the FUS/NR3C1 axis, thereby suppressing erastin-induced ferroptosis of BC cells.
Preclinical Investigation of Cavernous Nerve Injury-induced Erectile Dysfunction Treated With HAFSC-derived Secretome
Authors: Wen-Chun Hsu, Wei-Kung Tsai, Chun-Hou Liao, Chellappan Praveen Rajneesh, Xiao-Wen Tseng, Shiu-Dong Chung, Yi-No Wu
Journal: International Journal of Surgery (2025)
The double-edged sword of metabolic and bariatric surgery: extending the biliary limb can trigger bacterial translocation, sepsis, and liver inflammation – an experimental study
Authors: Louis Onghena, Anneleen Heldens, Kim De Paepe, Milton Antwi, Hans Van Vlierberghe, Sarah Raevens, Xavier Verhelst, Anne Hoorens, Lindsey Devisscher, Yves van Nieuwenhove, Tom Van de Wiele, Anja Geerts, Sander Lefere
Journal: International Journal of Surgery (2025)
Nanomedical Strategies for Kidney Disease: Diagnostic Innovations and Therapeutic Advancements
Authors: Fengyang Yao, Weiqi Gao, Limeng Li, Yijun Huang, Wei Sang, Ruiping Zhang
Journal: Advanced Healthcare Materials (2025)

Abstract Kidney diseases, posing significant global public health challenges due to their complex pathogenesis and diagnostic/therapeutic difficulties, have seen emerging advancements through nanomedicine. In diagnostics, nanoparticles leverage unique physicochemical properties to enhance imaging precision. Superparamagnetic iron oxide nanoparticles improve magnetic resonance imaging sensitivity by amplifying T 2 ‐weighted contrast, while microbubbles/nanobubbles enhance ultrasound resolution via signal reflection. Quantum dots and gold nanoparticles optimize photoacoustic imaging with superior fluorescence and photostability. Therapeutically, nanoparticle‐based drug delivery systems demonstrate targeted delivery, reduced systemic toxicity, and improved drug stability and bioavailability in preclinical studies. Nanocarrier‐integrated stem cell and gene therapies further show potential in repairing renal cells and mitigating kidney injury. This review systematically examines nanomedicine's dual diagnostic and therapeutic roles in kidney diseases, compares strengths and limitations of various nanodelivery platforms, and addresses current challenges in clinical translation. By exploring novel nanotechnology‐driven strategies, it aims to guide future research toward effective, tailored therapies for improved renal disease management.
Engineered 3D Kidney Glomerular Microtissues to Model Podocyte‐Centric Diseases for the Validation of New Drug Targets
Authors: Sara Buttό, Solange Vidal, Xi Rui Zeng, Martin Rausch, Immihan Ceren Yasa, Magali P. Ferro
Journal: Advanced Healthcare Materials (2025)

Abstract Podocytopathies are a diverse group of kidney diseases characterized by podocyte injury, leading to proteinuria and reduced kidney function. This injury often disrupts cytoskeletal dynamics and cellular adhesion, causing glomerular dysfunction. Current in vitro models fail to accurately mimic the three‐dimensional (3D) organization and mechanics of kidney tissue, hindering the understanding of podocyte pathophysiology and therapeutic development. In this study, 3D microtissues are developed that replicate the structure and mechanics of the glomerular filtration barrier, enabling the modeling of chemically and genetically induced podocyte injuries for drug target validation. These microtissues simulate the glomerulus's three‐layer structure and hemodynamic mechanical stretch, providing a platform to evaluate relevant mechanobiological signaling pathways and podocyte dynamics. Collective cellular forces are measured to assess podocyte resilience against genetic or chemical injuries. As a proof‐of‐concept, podocyte injury is modeled through transient receptor potantial canonical 6 (TRPC6) overexpression, a validated target in podocytopathies, and evaluated by the TRPC6 inhibitor SAR7334. The results demonstrated a loss of podocyte contractile forces upon TRPC6 overexpression, with recovery following treatment. This highlights the potential of glomerular microtissues to model podocyte mechano‐pathophysiology and serves as a robust platform for screening new therapies.
<i>DLGAP5</i> mutations Disrupt Normal Chromosome Segregation and Spindle Formation of human Oocyte Meiosis and Lead to Female Infertility
Authors: Meng Wang, Zhou Li, Juepu Zhou, Rui Long, Qingsong Xi, Hong Gao, Youzhu Li, Lei Jin, Lixia Zhu
Journal: MedComm (2025)
The Long Noncoding RNA LINC02820 Promotes Tumor Growth and Metastasis Through Regulating MYH9 Expression in Esophageal Squamous Cell Carcinoma
Authors: Xiaomin Xu, Xinting Mao, Wenrong Liu, Yue Ming, Tingting Zhang, Yang Yang, A‐Lai Gu‐Ha, Yi‐Dan Lin, Yong Peng
Journal: MedComm (2025)

Abstract Long noncoding RNAs (lncRNAs) play important roles in tumorigenesis, but their biological functions and mechanisms in esophageal squamous cell carcinoma (ESCC) remain poorly understood. In this study, we employed high‐throughput sequencing and bioinformatics analyses to identify the differentially expressed lncRNAs between ESCC tumors and adjacent normal tissues, among which LINC02820 is significantly upregulated in ESCC. Rapid amplification of cDNA ends assays determined the transcription initiation and termination sites of LINC02820, confirming it as a novel transcript variant localized in both the nucleus and cytoplasm of ESCC cells. Functional studies demonstrated that LINC02820 promotes cell proliferation and migration in vitro and enhances tumor growth and metastasis in vivo. Mechanistically, LINC02820 interacts with Myosin‐9 protein and prevent it from ubiquitination‐mediated proteasomal degradation. Additionally, the RNA‐binding protein insulin‐like growth factor 2 mRNA‐binding protein 2 binds to LINC02820 and increase its RNA stability in ESCC cells, thus upregulating LINC02820 expression. Therefore, these findings indicate LINC02820 as an oncogenic lncRNA in ESCC progression and suggest its potential as a therapeutic target.
Amplification of cGAS-STING Pathway with “Single-molecule Multitarget” Nanoparticles for Chemo-immunotherapy of Ovarian Cancer
Authors: Huijiao Fu, Xue Liu, Feng Fang, Zhiyin Pan, Longfei Chen, Gui Chen, Xin Yang, Wei Wang, Bo Yang, Jiemiao Chen, Xinning Yang, Zhiqiang Yu, Dong Liang, Songhua Yuan
Journal: Biomaterials (2025)
Classification grid and evidence matrix for evaluating digital medical devices under the European union landscape
Authors: Magali Boers, Aude Rochereau, Louisa Stuwe, Lorena San Miguel, Jochen Klucken, Fruzsina Mezei, Jérôme Fabiano, Sandrine Boulet, Aymeric Perchant, Rosanna Tarricone, Francesco Petracca, Barbara Hoefgen, Corinne Collignon, Sarah Zohar
Journal: npj Digital Medicine (2025)

A uniform and harmonised taxonomy of Digital Medical Devices (DMDs) and their evidence-based evaluation are essential to ensure their integration into healthcare systems across the European Union (EU). As part of the Taskforce for Harmonised Evaluation of DMDs, a Common European Classification Grid for DMDs (CEUGrid-DMD) associated with an Evidence Matrix is developed. These tools are based on the mapping of existing frameworks, a survey of Health Technology Assessment (HTA) practices, consensus meetings and workshop. The survey was sent to 32 national representatives of HTA bodies from 18 EU countries. Ten HTA bodies from nine countries completed the survey while others could not, in the absence of the effective implementation of a DMD evaluation framework. This work results in the CEUGrid-DMD including four taxonomy categories, associated with an evidence-based matrix. Overall, this first version should help to converge scientific assessments of DMDs in the context of HTA Regulations across the EU.
Corrigendum to “CXCL10 plays a key role as an inflammatory mediator and a non-invasive biomarker of non-alcoholic steatohepatitis” [J Hepatol (2014) 61:1365-75]
Authors: Xiang Zhang, Jiayun Shen, Kwan Man, Eagle S H Chu, Tung On Yau, Joanne C Y Sung, Minnie Y Y Go, Jun Deng, Liwei Lu, Vincent W S Wong, Joseph J Y Sung, Geoffrey Farrell, Jun Yu
Journal: Journal of Hepatology (2025)