tbuckley/cabot-search huggingface dataset:
Dual‐Enhanced Nanohybrids for Synergistic Photothermal and Photodynamic Therapy in Cancer Treatment with Immune Checkpoint Inhibitors
Authors: Dabin Lee, Hyungjin Sun, Jieun Bang, Tae‐Young Heo, Duong Thi‐Thuy Pham, Jong Dae Jang, Young Soo Han, Tae Joo Shin, Soo‐Hyung Choi, JaeHong Park, Junsang Doh, Juhyun Park
Journal: Advanced Healthcare Materials (2024)

Abstract This study presents a nanohybrid that simultaneously improves both photothermal (PT) and photodynamic (PD) effects for cancer therapy. The conjugated polymer nanoparticle (CPN) comprises of p‐type conjugated polymer as a photosensitizer, charge donor, and PT agent, n‐type conjugated polymer as a charge acceptor and PD agent, and Au nanoparticles (NPs) as a PT agent. This nanohybrid is assembled through a film dispersion process using a hydrophobically modified phospholipid, producing a high yield of uniform hybrid NPs in a short timeframe, and displays exceptional photothermal and photodynamic effects, when activated at a single near‐infrared wavelength. Photophysical analysis indicates that the inclusion of Au NPs enhances nonradiative exciton relaxation, while the incorporation of a n‐type conjugated polymer boosts photoinduced charge transfer and potentially contributes to the charge‐recombination mediated triplet‐state formation for an enhanced generation of reactive oxygen species. During phototherapy, the nanohybrid demonstrates the most effective suppression of primary tumor growth and significantly boosts anti‐tumor immune responses owing to its simultaneous photothermal and photodynamic effects. Furthermore, when combined with immune checkpoint inhibitors, nanohybrid treatment minimizes tumor sizes while maximizing survival rates in mice. Thus, the nanohybrid represents a promising nanoplatform for combination phototherapy in cancer treatment.
Cancer liquid biopsies by Oxford Nanopore Technologies sequencing of cell-free DNA: from basic research to clinical applications
Authors: Hua-Qi Si, Peng Wang, Fei Long, Wei Zhong, Yuan-Dong Meng, Yuan Rong, Xiang-Yu Meng, Fu-Bing Wang
Journal: Molecular Cancer (2024)

Liquid biopsies, in particular, analysis of cell-free DNA, are expected to revolutionize the current landscape of cancer diagnostics and treatment. However, the existing methods for cfDNA-based liquid biopsies for cancer have certain limitations, such as fragment interruption and GC bias, which are likely to be resolved by the emerging Oxford Nanopore Technologies (ONT), characterized by long read-length, fast read-times, high throughput, and polymerase chain reaction-free. In this review, we summarized the current literatures regarding the feasibility and applications of cfDNA-based liquid biopsies using ONT for cancer management, a possible game-changer that we believe is promising in detecting multimodal biomarkers and can be applied in a wide range of oncology utilities including early screening, diagnosis, and treatment monitoring.
Aquaporin-4 as a cerebrospinal fluid biomarker of Alzheimer’s disease
Authors: Nerea Gómez de San José, Steffen Halbgebauer, Petra Steinacker, Sarah Anderl-Straub, Samir Abu-Rumeileh, Lorenzo Barba, Patrick Oeckl, Giovanni Bellomo, Lorenzo Gaetani, Andrea Toja, Sára Mravinacová, Sofia Bergström, Anna Månberg, Alberto Grassini, Innocenzo Rainero, Peter Nilsson, Lucilla Parnetti, Markus Otto
Journal: Translational Neurodegeneration (2024)
Natural language processing in mixed-methods evaluation of a digital sleep-alcohol intervention for young adults
Authors: Frances J. Griffith, Garrett I. Ash, Madilyn Augustine, Leah Latimer, Naomi Verne, Nancy S. Redeker, Stephanie S. O’Malley, Kelly S. DeMartini, Lisa M. Fucito
Journal: npj Digital Medicine (2024)

We used natural language processing (NLP) in convergent mixed methods to evaluate young adults' experiences with Call it a Night (CIAN), a digital personalized feedback and coaching sleep-alcohol intervention. Young adults with heavy drinking (N = 120) were randomized to CIAN or controls (A + SM: web-based advice + self-monitoring or A: advice; clinicaltrials.gov, 8/31/18, #NCT03658954). Most CIAN participants (72.0%) preferred coaching to control interventions. Control participants found advice more helpful than CIAN participants (X2 = 27.34, p < 0.001). Most participants were interested in sleep factors besides alcohol and appreciated increased awareness through monitoring. NLP corroborated generally positive sentiments (M = 15.07(10.54)) and added critical insight that sleep (40%), not alcohol use (12%), was a main participant motivator. All groups had high adherence, satisfaction, and feasibility. CIAN (Δ = 0.48, p = 0.008) and A + SM (Δ = 0.55, p < 0.001) had higher reported effectiveness than A (F(2, 115) = 8.45, p < 0.001). Digital sleep-alcohol interventions are acceptable, and improving sleep and wellness may be important motivations for young adults.
<i>Bacteroides thetaiotaomicron</i> enhances H <sub>2</sub> S production in <i>Bilophila wadsworthia</i>
Authors: Jade Davies, Melinda J. Mayer, Nathalie Juge, Arjan Narbad, Lizbeth Sayavedra
Journal: Gut Microbes (2024)

Sulfate- and sulfite-reducing bacteria (SRB) are a group of strict anaerobes found within the human gut.
Re: Hondur et al.: Vitreopapillary findings in nonarteritic ischemic optic neuropathy versus healthy eyes: a clinical and OCT comparison. (Ophthalmology. 2024 Sep 12:S0161-6420(24)00557-8. doi: 10.1016/j.ophtha.2024.09.008. Epub ahead of print. PMID: 39276872)
Authors: M Tariq Bhatti
Journal: Ophthalmology (2024)
Author correction to ‘Generation of αGal-enhanced bifunctional tumor vaccine’ [Acta Pharmaceutica Sinica B 12 (2022) 3177-3186]
Authors: Jian He, Yu Huo, Zhikun Zhang, Yiqun Luo, Xiuli Liu, Qiaoying Chen, Pan Wu, Wei Shi, Tao Wu, Chao Tang, Huixue Wang, Lan Li, Xiyu Liu, Yong Huang, Yongxiang Zhao, Lu Gan, Bing Wang, Liping Zhong
Journal: Acta Pharmaceutica Sinica B (2024)

[This corrects the article DOI: 10.1016/j.apsb.2022.03.002.].
Sex and common germline variants impact the toxicity profile and pharmacokinetics of alectinib: a nation-wide cohort study in patients with ALK-positive non-small cell lung cancer
Authors: Niels Heersche, Daan A C Lanser, M Benthe Muntinghe-Wagenaar, Ma Ida Mohmaed Ali, Ezgi B Ulas, Tessa M A Trooster, Evert de Jonge, Esther Oomen-de Hoop, Marthe S Paats, Idris Bahce, Sander Croes, Lizza E L Hendriks, Anthonie J van der Wekken, Anne-Marie C Dingemans, Alwin D R Huitema, Ron H N van Schaik, Ron H J Mathijssen, G D Marijn Veerman
Journal: Journal of Thoracic Oncology (2024)
Endoplasmic reticulum stress-related deficits in calcium clearance promote neuronal dysfunction that is prevented by SERCA2 gene augmentation
Authors: Yukihiro Shiga, Aline Giselle Rangel Olguin, Sana El Hajji, Nicolas Belforte, Heberto Quintero, Florence Dotigny, Luis Alarcon-Martinez, Arjun Krishnaswamy, Adriana Di Polo
Journal: Cell Reports Medicine (2024)
Features of hyperinflammation link the biology of EBV infection and cytokine storm syndromes
Authors: Meng Liu, Kailey E Brodeur, Jacob R Bledsoe, Claudia N Harris, Jill Joerger, Rachel Weng, Evan E Hsu, Michael T Lam, Casey A Rimland, Courtney E LeSon, Jian Yue, Lauren A Henderson, Fatma Dedeoglu, Jane W Newburger, Peter A Nigrovic, Mary Beth F Son, Pui Y Lee
Journal: Journal of Allergy and Clinical Immunology (2024)
Consensus of the Italian Primary Immunodeficiency Network on the use and interpretation of genetic testing for the diagnosis of inborn errors of immunity (IEI)
Authors: Giuliana Giardino, Gigliola Di Matteo, Silvia Giliani, Simona Ferrari, Vassilios Lougaris, Raffaele Badolato, Francesca Conti, Roberta Romano, Maria Pia Cicalese, Silvia Ricci, Federica Barzaghi, Antonio Marzollo, Cristina Cifaldi, Davide Montin, Lorenzo Lodi, Emilia Cirillo, Baldassarre Martire, Antonio Trizzino, Mayla Sgrulletti, Viviana Moschese, Marika Comegna, Giuseppe Castaldo, Alberto Tommasini, Chiara Azzari, Caterina Cancrini, Alessandro Aiuti, Claudio Pignata
Journal: Journal of Allergy and Clinical Immunology (2024)
Inborn errors of immunity reveal the molecular requirements for the generation and maintenance of human IL-9 expressing cells.
Authors: Geetha Rao, Corinne D Mack, Tina Nguyen, Natalie Wong, Kathryn Payne, Lisa Worley, Paul E Gray, Melanie Wong, Peter Hsu, Michael O Stormon, Kahn Preece, Daniel Suan, Michael O'Sullivan, Annaliesse K Blincoe, Jan Sinclair, Satoshi Okada, Sophie Hambleton, Peter D Arkwright, Kaan Boztug, Polina Stepensky, Megan A Cooper, Liliana Bezrodnik, Kari C Nadeau, Hassan Abolhassani, Roshini S Abraham, Mikko R J Seppänen, Vivien Béziat, Jacinta Bustamante, Lisa R Forbes Satter, Jennifer W Leiding, Isabelle Meyts, Emmanuelle Jouanguy, Stéphanie Boisson-Dupuis, Gulbu Uzel, Anne Puel, Jean-Laurent Casanova, Stuart G Tangye, Cindy S Ma
Journal: Journal of Allergy and Clinical Immunology (2024)
Expert consensus recommendations for the diagnosis and treatment of chronic non-bacterial osteitis (CNO) in adults
Authors: Elizabeth M Winter, Olaf M Dekkers, Caroline M Andreasen, Salvatore D'Angelo, Natasha M Appelman-Dijkstra, Simone Appenzeller, Gunter Assmann, Judith S Bubbear, Oana O Bulaicon, Roland Chapurlat, Varvara Choida, Gavin Peter Ross Clunie, Dimitrios Daoussis, Torsten Diekhoff, Marcel Flendrie, Olivier Fogel, Roba Ghossan, Hermann Girschick, Femke van Haalen, Neveen A T Hamdy, Barbara Hauser, Christian M Hedrich, Philip S Helliwell, Kay Geert Hermann, Antonella Insalaco, Anne Grethe Jurik, Mitsumasa Kishimoto, Willem Lems, Paivi Miettunen, Burkhard Muche, Ana Navas Cañete, Natalia Palmou-Fontana, Frits Smit, James Teh, Charlotte Verroken, Kurt de Vlam, Daniel Wendling, Wei Zhou, Hans-Georg Zmierczak, Anne T Leerling
Journal: Annals of the Rheumatic Diseases (2025)

There is considerable practice variation in labelling, diagnosis and treatment of adults with sterile bone inflammation. We developed a expert consensus recommendations on the disease definition, diagnosis and treatment of this rare condition. Systematic literature review and Grading of Recommendations, Assessment, Development and Evaluations-based appraisal of evidence, two Delphi surveys and three digital and in-person consensus meetings with a multidisciplinary expert panel and patient representatives. A consensus disease definition was developed and the term 'chronic non-bacterial osteitis' (CNO) is proposed to describe adults with sterile bone inflammation. For initial imaging evaluation of adults with suspected CNO, the panel recommends MRI or otherwise CT combined with nuclear imaging. Whole-body imaging at initial evaluation can be considered for diagnostic and prognostic purposes. Suggested first-line treatment in adults with active CNO includes nonsteroidal anti-inflammatory drugs/cyclooxygenase 2-inhibitors. Second-line treatment preferably consists of intravenous bisphosphonates, and otherwise tumour necrosis factor-α inhibitors. Choice between them should be individualised, considering the presence of additional inflammatory features. The panel further discusses outcome measures, follow-up and management of adverse events and complications. These expert consensus recommendations are intended to support healthcare professionals worldwide in their care for adults with CNO. They also lay the groundwork for establishing international patient registries, translational research lines and multicentre trials, all of which are urgently required.
Sparse outer longitudinal muscle layer in peranal endoscopic myectomy: reinforcement of wound closure with peptide gel
Authors: Yoshiaki Ando, Takashi Kanesaka, Tomoki Michida, Ryu Ishihara
Journal: Endoscopy (2024)

Peranal endoscopic myectomy (PAEM) is a novel endoscopic technique for removing lesions together with the inner circular muscle layer in patients with lower rectal tumors with severe submucosal fibrosis [1] [2] [3] [4]. In this procedure, the outer longitudinal muscle is exposed at the resection area and its fragility poses a risk for perforation. As there are few reports on PAEM, the thickness of the muscles in the lower rectum is not well known. In our experience, the outer longitudinal muscle is usually dense and the extraluminal tissue is invisible ([Fig. 1]). Herein, we present the management of a patient with a sparse outer longitudinal muscle layer ([Video 1]).
Idiopathic inflammatory myopathies related lung disease in adults
Authors: Sameep Sehgal, Aditi Patel, Soumya Chatterjee, Anthony P Fernandez, Carol Farver, Ruchi Yadav, Yuebing Li, Sonye K Danoff, Didem Saygin, Julio A Huapaya, Erin M Wilfong, Kristin B Highland
Journal: The Lancet Respiratory Medicine (2024)
Impact of human and artificial intelligence collaboration on workload reduction in medical image interpretation
Authors: Mingyang Chen, Yuting Wang, Qiankun Wang, Jingyi Shi, Huike Wang, Zichen Ye, Peng Xue, Youlin Qiao
Journal: npj Digital Medicine (2024)

Clinicians face increasing workloads in medical imaging interpretation, and artificial intelligence (AI) offers potential relief. This meta-analysis evaluates the impact of human-AI collaboration on image interpretation workload. Four databases were searched for studies comparing reading time or quantity for image-based disease detection before and after AI integration. The Quality Assessment of Studies of Diagnostic Accuracy was modified to assess risk of bias. Workload reduction and relative diagnostic performance were pooled using random-effects model. Thirty-six studies were included. AI concurrent assistance reduced reading time by 27.20% (95% confidence interval, 18.22%–36.18%). The reading quantity decreased by 44.47% (40.68%–48.26%) and 61.72% (47.92%–75.52%) when AI served as the second reader and pre-screening, respectively. Overall relative sensitivity and specificity are 1.12 (1.09, 1.14) and 1.00 (1.00, 1.01), respectively. Despite these promising results, caution is warranted due to significant heterogeneity and uneven study quality.
Establishing responsible use of AI guidelines: a comprehensive case study for healthcare institutions
Authors: Agustina D. Saenz, Thomas McCoy, Allison Bryant Mantha, Robert Martin, Robert Damiano, Deborah Adair, David Heaney, Rachel Sisodia, Lee Park, Robert Forsberg, Gerry Tuffy, Shawn Murphy, Keith Dreyer, Martha Jones, Heather Jean Cosier, Merranda Logan, Yogendra Bundela, Amanda Centi, David Ting, Jacqueline G. You, Adam Landman, Rebecca G. Mishuris
Journal: npj Digital Medicine (2024)

This report presents a comprehensive case study for the responsible integration of artificial intelligence (AI) into healthcare settings. Recognizing the rapid advancement of AI technologies and their potential to transform healthcare delivery, we propose a set of guidelines emphasizing fairness, robustness, privacy, safety, transparency, explainability, accountability, and benefit. Through a multidisciplinary collaboration, we developed and operationalized these guidelines within a healthcare system, highlighting a case study on ambient documentation to demonstrate the practical application and challenges of implementing generative AI in clinical environments. Our proposed framework ensures continuous monitoring, evaluation, and adaptation of AI technologies, addressing ethical considerations and enhancing patient care. This work contributes to the discourse on responsible AI use in healthcare, offering a blueprint for institutions to navigate the complexities of AI integration responsibly and effectively, thus promoting better, more equitable healthcare outcomes.
Identifying Parkinson’s disease and its stages using static standing balance
Authors: Dawoon Jung, Dallah Yoo, Jinwook Kim, Tae-Beom Ahn, Kyung-Ryoul Mun
Journal: npj Digital Medicine (2024)

The current assessment of Parkinson's disease (PD) relies on dynamic motor tasks, limiting accessibility. This study aimed to propose an innovative approach to identifying PD and its stages using static standing balance and machine learning. A total of 210 participants were recruited, including a control group and five PD groups categorized by stage. Each participant completed a 10-s static standing balance task in which center of pressure trajectory data in the medial-lateral and anterior-posterior directions were collected. Features were extracted from these trajectory data and the data derived from them using both representation learning and handcrafting methods. A Transformer encoder-based classifier was trained on these features and achieved an F1-score of 0.963 in classifying the six study groups. This approach enhances the accessibility of PD assessment, enabling earlier detection and timely intervention. The novel data mining framework introduced in this study heralds a new era of time-series data-driven digital healthcare.
Phenotyping people with a history of injecting drug use within electronic medical records using an interactive machine learning approach
Authors: Carol El-Hayek, Thi Nguyen, Margaret E. Hellard, Michael Curtis, Rachel Sacks-Davis, Htein Linn Aung, Jason Asselin, Douglas I. R. Boyle, Anna Wilkinson, Victoria Polkinghorne, Jane S. Hocking, Adam G. Dunn
Journal: npj Digital Medicine (2024)
Nanoparticulate drug combination inhibits DNA damage repair and PD-L1 expression in BRCA-mutant and wild type triple-negative breast cancer
Authors: Ibrahim Alradwan, Pei Zhi, Tian Zhang, HoYin Lip, Abdulmottaleb Zetrini, Chunsheng He, Jeffrey T Henderson, Andrew M Rauth, Xiao Yu Wu
Journal: Journal of Controlled Release (2024)
Protocol for engineering bone organoids from mesenchymal stem cells
Authors: Jian Wang, Dongyang Zhou, Ruiyang Li, Shihao Sheng, Guangfeng Li, Yue Sun, Peng Wang, Yulin Mo, Han Liu, Xiao Chen, Zhen Geng, Qin Zhang, Yingying Jing, Long Bai, Ke Xu, Jiacan Su
Journal: Bioactive Materials (2024)
NIR‐II Photoacoustic Imaging‐Guided Chemo‐Photothermal Therapy Using PA1094T Combined with Anti‐CD47 Antibody: Activating Pyroptosis against Orthotopic Glioblastoma
Authors: Shiying Li, Fanchu Zeng, Qi Zhou, Lanqing Li, Hsuan Lo, Jiali Chen, Zhijin Fan, Guojia Huang, Liming Nie
Journal: Advanced Healthcare Materials (2024)

Abstract Treating glioblastoma (GBM) with single‐agent chemotherapy is often ineffective due to inefficient drug delivery and the immunosuppressive tumor microenvironment, which leads to drug resistance. Strategies that activate programmed cell death mechanisms and repolarized tumor‐associated macrophages toward an antitumoral M1‐like phenotype can help reverse the immunosuppressive tumor microenvironment. In this study, a novel approach using NIR‐II (1000–1700 nm) photoacoustic imaging (PAI)‐guided chemo‐photothermal therapy is presented. NIR‐II imaging, with its superior tissue penetration and reduced background noise, enables precise tumor targeting. A targeted nano prodrug is developed using poly (lactic ‐co ‐glycolic acid) nanoparticles loaded with A1094 dye and temozolomide (TMZ), coupled with an anti‐CD47 antibody. This system employs synergistic chemo‐photothermal therapy activated by NIR‐II light, inducing apoptosis, pyroptosis, and T‐cell activation. PAI provides rapid, point‐of‐care GBM diagnosis, and highlighted the effective targeting of the PA1094T nanoplatform. In a recurrent GBM model, the combination of PA1094T and anti‐CD47 antibody significantly enhances cancer cell phagocytosis and effectively remodels the immunosuppressive microenvironment, resulting in better therapeutic outcomes compared to conventional therapies. These results indicate that this NIR‐II PAI‐guided drug cocktail therapy is a promising strategy for treating GBM, potentially addressing drug resistance and improving treatment efficacy through enhanced targeting and immunomodulation.
RELAY: Final Overall Survival for Erlotinib + Ramucirumab or Placebo in Untreated, EGFR-Mutated Metastatic NSCLC
Authors: Kazuhiko Nakagawa, Edward B Garon, Takashi Seto, Makoto Nishio, Santiago Ponce Aix, Luis Paz-Ares, Chao-Hua Chiu, Keunchil Park, Silvia Novello, Ernest Nadal, Kazumi Nishino, Kiyotaka Yoh, Jin-Yuan Shih, Jeannie Yk Chik, Denis Moro-Sibilot, Tarun Puri, Sunoj Chacko Varughese, Bente Frimodt-Moller, Carla Visseren-Grul, Martin Reck
Journal: Journal of Thoracic Oncology (2024)
Receptor tyrosine kinase fusion-mediated resistance to EGFR-TKI in EGFR-mutant NSCLC: a multi-center analysis and literature review
Authors: Yang Xia, Kaiwen Wang, Jing Zhao, Zhaohui Arter, Yongchang Zhang, Jiaqi Zhou, Yuefei Lu, Liang Zeng, Robyn Du, Jennifer A Owens, Yasir Y Elamin, Carl M Gay, Ferdinandos Skoulidis, Anne S Tsao, Charles Lu, Tina Cascone, Don L Gibbons, Jianjun Zhang, Olivia Chen, Kevin Ks Mok, Misako Nagasaka, Wen Li, John V Heymach, Sai-Hong Ignatius Ou, Molly Li, Xiuning Le
Journal: Journal of Thoracic Oncology (2024)

Drug resistance remains a significant challenge in EGFR-mutant NSCLC tumors engage due to pathway reactivation, pathway bypass and pathway indifference resistance mechanisms to evade tyrosine kinase inhibitor (TKI) suppression. Fusion of receptor tyrosine kinases (RTKs), such as RET, ALK, and FGFR3, has been reported to mediate EGFR TKI resistance. Given the rarity of these fusions and heterogenous nature of the condition, no prospective clinical trials evaluated the incidence, safety, and therapeutic benefit of dual EGFR-RTK inhibition.
Re: Muayad et al.: Comparative effects of GLP-1 receptor agonists and metformin on glaucoma risk in type 2 diabetes patients (Ophthalmology. Aug 23 2024;doi:10.1016/j.ophtha.2024.08.023)
Authors: Shu-Han Chuang, Cheng-Hsien Chang
Journal: Ophthalmology (2024)
Corrigendum to “Optical coherence tomography in the management of diabetic macular oedema” [Prog. Retin. Eye Res. 98 (2024) 101220]
Authors: Simon Kh Szeto, Timothy Yy Lai, Stela Vujosevic, Jennifer K Suns, SriniVas R Sadda, Gavin Tan, Sobha Sivaprasad, Tien Y Wong, Carol Y Cheung
Journal: Progress in Retinal and Eye Research (2024)
N-terminal acetylation of transcription factor LIP induces immune therapy resistance via suppression of PD-L1 expression in non-small cell lung cancer
Authors: Xiang He, Yongshuo Liu, Xing Gao, Feiyu Tang, Yuxi Tian, Siyuan Gong, Jia Shen, Aimin Wang, Lunquan Sun, Wensheng Wei, Liang Weng
Journal: Journal for ImmunoTherapy of Cancer (2024)

Background Programmed death-1 (PD-1) checkpoint blockade has revolutionized cancer therapy, yet its clinical success is confined to a subset of patients, underscoring the urgent need to understand the molecular underpinnings of programmed cell death ligand 1 (PD-L1) expression to combat immunotherapy resistance. Methods Employing CRISPR/Cas9 screening, we identified key regulators of PD-L1 in non-small cell lung cancer (NSCLC) cells, focusing on the transcription factor CEBPB and its isoform liver-enriched inhibitory protein (LIP). Through chromatin immunoprecipitation (ChIP) and luciferase reporter assays, we explored the interaction between LIP and basic-helix-loop-helix E22 (BHLHE22) in controlling PD-L1 transcription. We also used immunofluorescence and NBD-CI assays to examine how N-terminal acetylation affects LIP’s subcellular localization. The impact of LIP on tumor growth was assessed via subcutaneous tumorigenicity assays, while immunohistochemistry and immunofluorescence were used to analyze LIP-induced alterations in the tumor immune microenvironment. Results Our research indicates that CEBPB, particularly its LIP isoform, significantly suppresses PD-L1 expression in NSCLC cells. This suppression is contingent on LIP’s N-terminal acetylation by the N-terminal acetyltransferase A complex, which facilitates LIP’s nuclear entry and interaction with BHLHE22. This interaction leads to the formation of a co-repressor complex at the PD-L1 promoter, effectively reducing PD-L1 expression and enhancing the tumor immune response. Conclusions Identifying CEBPB, especially the LIP isoform, as a pivotal regulator of PD-L1 expression sheds light on the mechanisms behind PD-1 blockade resistance in NSCLC. Our findings suggest that modulating LIP’s function or its molecular interactions might offer a novel approach to boosting the efficacy of immunotherapies.