Abstract LB111: CORSA study - Telomere length measurement of 5371 Austrian colorectal cancer and adenoma patients and healthy controls: Relationships to tumor stage, course of disease and to clinical, life style and health parameters
Authors: Petr Hanák
Journal: Cancer Research (2024)

Abstract CORSA study - Telomere length measurement of 5371 Austrian colorectal cancer and adenoma patients and healthy controls: relationships to tumor stage, course of disease and to clinical, life style and health parametersPetr Hanak1, Michal Kroupa1,3,4, Uttarilli Anusha1, Ludmila Vodickova1,3,4 Andrea Gsur2, Stefanie Brezina2, Pavel Vodicka1,3,4 1 Department of Molecular Biology of Cancer, Institute of Experimental Medicine of the Czech Academy ofSciences, Videnska 1083, 142 20 Prague, Czech Republic.2 Center for Cancer Research, Medical University of Vienna, Vienna, Austria;3 Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic 4Faculty of Medicine and Biomedical center in Pilsen, Pilsen, Czech Republic Presenting author: Petr Hanák (e-mail: petr.hanak@iem.cas.cz)Telomeres are important parts of human chromosomes, playing a pivotal role in maintenance of genomic integrity. If their function is perturbed, it may trigger many pathologies, including colorectal cancer (CRC). In the present study, comparison of assessed relative telomere length (RTL) and tumor stage, progression of the disease and various health status and lifestyle parameters is investigated. Relative telomere length was measured in samples of DNA from peripheral blood lymphocytes of CRC patients by monochromatic multiplex qPCR. Repetitive telomere motif TTAGG was amplified from specific primer pair simultaneously with the reference albumin gene, the second one amplified from another set of primers specific to this gene. The qPCR reaction is based on intercalating dye Syto 9, which does not distinguish specific target. A separation of amplification of one target from the other one is based on division in time of both amplifications and on different levels of annealing temperatures of primer pairs. Relative telomere length was assessed in all patient samples. Obtained data correspond to our previously observed values. Here, we observe significant decreasing in RTL in the order healthy controls, adenoma and CRC patients (controls vs adenoma patients P&amp;lt;0.0001, controls vs CRC patients P=0.0001). Detailed statistical analyses and assignment to all relevant groups including health and lifestyle parameters is currently in progress. Acknowledgement &amp; Funding: The study was funded by the Grant Agency of Czech Republic (21-27902S), National Institute for Cancer Research (Programme EXCELES, ID Project No. LX22NPO5102) - Funded by the European Union – Next Generation EU and Ministry of Health of the Czech Republic (NU22J-03-00028) Citation Format: Petr Hanák. CORSA study - Telomere length measurement of 5371 Austrian colorectal cancer and adenoma patients and healthy controls: Relationships to tumor stage, course of disease and to clinical, life style and health parameters [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB111.