tbuckley/cabot-search huggingface dataset:
Memory-promoting function of miR-379-5p attenuates CD8<sup>+</sup>T cell exhaustion by targeting immune checkpoints
Authors: You-Zhe Lin, Chia-Hsin Liu, Wan-Rong Wu, Ting-Yi Liao, Chuan-Chun Lee, Hong-Wei Li, Feng-Chi Chung, Yi-Chun Shen, Guan-Yu Zhuo, Liang-Chih Liu, Wei-Chung Cheng, Shao-Chun Wang
Journal: Journal for ImmunoTherapy of Cancer (2025)

MicroRNAs (miRNAs) are epigenetic regulators of T cell maturation and exhaustion. However, the mechanisms by which miRNAs influence T cell function in tumor environments remain unclear. This study focuses on miR-379-5p, which counteracts T cell exhaustion and enhances antitumor responses. Native CD8+ T cells were isolated from the blood of healthy donors and subjected to chronic stimulation to induce exhaustion. RNA sequencing and miRNA sequencing were performed to identify differentially expressed miRNAs. These miRNAs underwent bioinformatics analyses, including DESeq enrichment, immune cell infiltration assessment, and patient prognostic outcomes in The Cancer Genome Atlas data sets to assess their potential involvement in T cell exhaustion and antitumor immunity. The biological functions of miRNA on T cell differentiation, cytotoxic killing, and immune checkpoint regulation were investigated using in vitro assays, OT-I B16F10-OVA models, and patient-derived tumor organoids. MiR-379-5p is downregulated in exhausted T cells and negatively associated with exhausted tumor-infiltrating lymphocytes in advanced tumors. It correlates positively with better survival outcomes in breast cancer, cervical cancer and melanoma. In CD8+ T cells, miR-379-5p reduces the expression of immune checkpoint proteins T cell immunoglobulin and mucin-domain containing-3 (TIM3) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) by targeting their 3' untranslated region. Overexpression of miR-379-5p in CD8+ T cell promotes differentiation into memory-like T effector cells and enhances cytotoxic killing of cancer cells. The transcription factor nuclear receptor subfamily 4 group A member 1 (NR4A1) with increased expression in exhausted T cells and negatively regulates miR-379, restoring immune checkpoint expression and suppressing cancer-killing ability. In contrast, OT-I T cells expressing ectopic miR-379-5p show increased cytotoxicity against B16F10-OVA tumors in mice. Autologous T cells isolated from patients with breast cancer transduced with miR-379-5p significantly improve killing of tumor organoids derived from the same patients. MiR-379-5p acts as an epigenetic tumor suppressor by enhancing CD8+ T cell effector functions and suppressing T cell exhaustion. MiR-379-5p could represent a novel marker and strategy for cancer immunotherapy, offering promising avenues for enhancing antitumor immune responses.
Peripheral Nerve Schwannoma With Intense 68Ga PSMA Uptake
Authors: Muhammet Halil Baltacioglu, Burak Demir
Journal: Clinical Nuclear Medicine (2025)

Prostate-specific membrane antigen (PSMA) is a specific marker of the prostate cell lineage. 68Ga-PSMA PET/CT is a sensitive and valuable imaging modality used for staging at diagnosis and restaging in cases of recurrence during the follow-up of prostate carcinoma patients. However, increased PSMA uptake can be observed in various malignancies, such as cholangiocarcinoma, hepatocellular carcinoma, adenoid cystic carcinoma, renal cell carcinoma, and in several benign conditions, such as angiolipoma. Here, we present a case of a newly diagnosed prostate carcinoma patient with a schwannoma exhibiting intense 68Ga PSMA uptake mimicking paravertebral soft tissue metastasis of prostate cancer.
Pseudoprogression on 68Ga-DOTATATE PET/CT in a Pediatric Patient With Neuroblastoma
Authors: Xiaoya Wang, Siqi Li, Jun Liu, Jigang Yang
Journal: Clinical Nuclear Medicine (2025)

A 6-year-old boy with neuroblastoma underwent a 68Ga-DOTATATE PET/CT scan to assess his therapeutic response. The results revealed significant DOTATATE uptake in the right mandible ramus compared with previous scans, indicating potential disease progression. In contrast, 123I-MIBG SPECT/CT imaging demonstrated the resolution of this lesion when compared with earlier MIBG images. This case highlights the possibility of pseudoprogression on 68Ga-DOTATATE PET/CT during the repair process of bone metastatic lesions in neuroblastoma.
The therapeutic potential of mRNA-encoded SFTSV human monoclonal antibody encapsulated lipid nanoparticle in vivo
Authors: Soo-Yeon Lee, Yebeen Lee, Eun Young Oh, Jisun Lee, Jae-Yong Kim, Sang-In Park, Hyo-Jung Park, So Hyun Park, Eun-Jin Choi, Dahyeon Ha, Ayoung Oh, Ayeon Kim, Hyo-Jin Ro, Yoo-Jin Bang, Hye Won Kwak, Hyeong-Jun Park, Do-Hyung Kim, Daegeun Kim, Sang-Myeong Lee, Nam-Hyok Cho, Jae-Hwan Nam
Journal: Journal of Controlled Release (2025)
The R-RAS2 GTPase is a signaling hub in triple-negative breast cancer cell metabolism and metastatic behavior
Authors: Claudia Cifuentes, Lydia Horndler, Pilar Grosso, Clara L Oeste, Alejandro M Hortal, Jennifer Castillo, Isabel Fernández-Pisonero, Alberto Paradela, Xosé Bustelo, Balbino Alarcón
Journal: Journal of Hematology & Oncology (2025)

Recent research from our group has shown that the overexpression of the wild-type RAS-family GTPase RRAS2 drives the onset of triple-negative breast cancer (TNBC) in mice following one or more pregnancies. This phenomenon mirrors human TNBC, where RRAS2 is overexpressed in approximately 75% of cases, particularly in tumors associated with the postpartum period. These findings underscore the relevance of R-RAS2 in TNBC development and progression. We conducted RNA sequencing on tumors derived from conditional knock-in mice overexpressing human wild-type RRAS2 to identify the somatic mutation landscape associated with TNBC development in these mice. Additionally, we developed a TNBC cell line from RRAS2-overexpressing mice, enabling loss-of-function studies to investigate the role of R-RAS2 in various pathobiological parameters of TNBC cells, including cell migration, invasiveness, metabolic activity, and metastatic spread. Furthermore, proteomic analysis of a freshly isolated tumor identified plasma membrane receptors interacting with R-RAS2. Our findings demonstrate that TNBC driven by RRAS2 overexpression exhibits a pattern of somatic mutations similar to those observed in human breast cancer, particularly in genes involved in stemness, extracellular matrix interactions, and actin cytoskeleton regulation. Proteomic analysis revealed that wild-type R-RAS2 interacts with 245 membrane-associated proteins, including key solute carriers involved in cell metabolism (CD98/LAT1, GLUT1, and basigin), adhesion and matrix interaction proteins (CD44, EpCAM, MCAM, ICAM1, integrin-α6, and integrin-β1), and stem cell markers (β1-catenin, α1-catenin, PTK7, and CD44). We show that R-RAS2 regulates CD98/LAT1 transporter-mediated mTOR pathway activation and mediates CD44-dependent cancer cell migration and invasion, thus providing a mechanism by which R-RAS2 promotes breast cancer cell metastasis. R-RAS2 associates with CD44, CD98/LAT1, and other plasma membrane receptors to regulate metabolic activity, actin cytoskeleton reorganization, cell migration, invasion, and distant metastasis formation in TNBC. These findings establish R-RAS2 as a central driver of TNBC malignancy and highlight its potential as a promising therapeutic target, particularly in aggressive, postpartum-associated breast cancers.
Oxidized fucoidan-based nanocomposite hydrogel for cryptotanshinone delivery and prevention of postoperative abdominal adhesions
Authors: Tao Zhang, Xianmin Shi, Yanjuan Huang, Yujun Gong, Yuanfeng He, Danni Xiao, Shengzhi Wang, Chunshun Zhao
Journal: Journal of Controlled Release (2025)
Validation and expansion of Baveno VII recompensation criteria in patients with cirrhosis and curable liver disease
Authors: Marta Tonon, Roberta Gagliardi, Enrico Pompili, Anna Barone, Giacomo Zaccherini, Gianluca Zilio, Maurizio Baldassarre, Antonio Accetta, Daniele Carrello, Valeria Calvino, Giulia Iannone, Simone Incicco, Nicola Zeni, Carmine Gabriele Gambino, Paolo Caraceni, Paolo Angeli, Salvatore Piano
Journal: Journal of Hepatology (2025)
Histones post-translational modifications associated with the development of metabolic dysfunction-associated fatty liver disease. Part 3. Histon acetylation
Authors: O.E. Abaturov, A.O. Nikulina
Journal: GASTROENTEROLOGY (2025)

Based on the analysis of literature sources from the Pubmed, MEDLINE, The Cochrane Library, Embase databases, the authors of the article highlight general provisions regarding histone acetylation. They emphasize that it is acetylation of the lysine residue that is the key post-translational modification of histones by epigenetic mechanisms of gene expression regulation. To date, at least 2000 human proteins have been identified that can be modified at lysine residues by acetylation. About 1000 proteins in human liver tissue undergo modification by lysine acetylation. Acetylation is mediated by histone acetyltransferases (HAT/KAT) and is usually associated with active gene transcription due to its ability to decondense chromatin. In contrast, histone deacetylases (HDAC/KDAC) remove the acetyl group from the lysine residue of histones and, thus, restore the compact form of chromatin. The acetylation state of target sites is determined by the balance of activity between the processes of acetylation and deacetylation of the N-terminal regions of histone molecules. Histone hyperacetylation can be caused by both increased HAT/KAT activity and decreased HDAC expression. Histone acetyltransferases catalyze the transfer of an acetyl group from acetyl-CoA to the epsilon-amino group of lysine, neutralizing the positive charge of lysine and weakening the interaction between histones and the DNA molecule. Histone acetylation is always associated with chromatin opening and activation of gene transcription. The authors emphasize that an increase in the representation of acetylated markers is associated with the progression of metabolic dysfunction-associated fatty liver disease (MAFLD). A particularly high level of acetylation (hyperacetylation) in MAFLD is noted at sites K9, K14 and K18 of histone 3. Today, epigenetic mechanisms that determine the state of histone acetylation in the region of certain genes are considered as potential targets for drug treatment. Studying the influence of histone acetylation on morphological changes in tissue and the development of metabolic disorders is the basis that will allow developing effective epigenetic methods for treatment of patients with MAFLD.
Biochemical composition of refluxate in saliva and gastric juice in patients with physiological cardia insufficiency and hiatal hernias under martial law conditions
Authors: O.M. Babii, A.M. Halinska, N.V. Prolom, О.О. Halinskyi
Journal: GASTROENTEROLOGY (2025)

Background. A hiatal hernia (HH) and functional cardia insufficiency lead to changes in the biochemical composition of gastric juice and saliva, affecting the course of reflux disease and the condition of the mucous membrane. The study of biochemical markers such as pepsin, bile acids, total calcium, and nitric oxide (NOx) allows for a more accurate diagnosis of reflux types, assessment of the degree of mucosal damage, and identification of the di­seases pathogenesis features in both military (MP) and non-military personnel (NMP). This will contribute to the development of optimal approaches to diagnosis and treatment. Objective: to assess the biochemical composition of refluxate in hiatal hernias under martial law conditions by examining saliva and gastric juice. Materials and methods. The study was conducted among 66 patients divided by the types of HH: 30 military personnel: type I — axial HH (n = 11), type II — paraesophageal HH (n = 19); and 36 NMP: type I — axial HH (n = 22), type II — paraesophageal HH (n = 14). Biochemical analyses of saliva and gastric juice included pH measurement, determination of pepsin, bile acids, total calcium, and NOx levels. Results. MP with HH had an increase in pH and a decrease in pepsin concentration, indicating hypoacidity. In NMP with HH type I, these changes were more pronounced. The content of bile acids in gastric juice was elevated in all groups, particularly in MP with HH type II, indicating alkaline reflux. A significant increase in bile acid concentration was observed in the saliva of MP, which is a marker of aggressive reflux, whereas in NMP, pH levels changed more prominently, along with a reduction in pepsin. NOx content in MP with HH type II significantly exceeded control values, indicating active inflammatory processes. Conclusions. The results obtained confirm that among participants with physiological cardia insufficiency in HH, the composition of refluxate, the mechanisms and clinical features of reflux disease were different depending on the HH type and the professional status of the patient. Alkaline refluxate, high nitric oxide and total calcium concentrations are the dominant features of HH type II, especially in MP, whereas acidic refluxate was more commonly observed in MP with physiological cardia insufficiency in HH type I. This highlights the necessity of an individualized approach to the diagnosis and treatment of patients based on refluxate composition.
Complicated course of portal hypertension on the background of primary myelofibrosis: a clinical case
Authors: V.B. Yagmur, N.Yu. Zavhorodnia, S.L. Melanich, O.M. Babii, K.P. Lyapicheva, S.O. Tarabarov, M.D. Kushnir, D.V. Popok, N.P. Dementiy
Journal: GASTROENTEROLOGY (2025)

Esophageal varices are a consequence of portosystemic collaterals that develop under conditions of increased pressure between the portal and inferior vena cava. Bleeding from varices significantly worsens the patient’s condition and poses a risk to his life. Portal hypertension is usually considered as complication of liver cirrhosis, but there is a small pool of patients with so-called extrahepatic causes. The second most frequent cause of portal hypertension after cirrhosis is portal vein thrombosis. The article describes a case of clinically significant portal hypertension complicated by esophageal varices, which developed due to portal vein thrombosis after SARS-CoV-2 infection. During additional examination, liver cirrhosis was ruled out, and further clinical search was aimed at identifying other factors of thrombophilia. As a result of the morphological and genetic examination, a diagnosis of primary myelofibrosis was made. Thus, in patients with portal vein thrombosis, the development of clinically significant portal hypertension, the possibility of a myeloproliferative neoplasm should be considered after exclusion of cirrhosis.
Features of lipid metabolism in patients with erosive esophagitis during the period of martial law
Authors: L.M. Mosiychuk, I.A. Klenina, O.P. Petishko
Journal: GASTROENTEROLOGY (2025)

Background. Over the past decades, the number of patients with erosive esophagitis in the world has increased. At the same time, metabolic syndrome and chronic stress are considered risk factors for the development of erosive esophagitis. The aim of the study: to determine the diagnostic significance of lipid meta­bolism indicators for the formation of a group at risk of developing erosive esophagitis among patients with gastroesophageal reflux disease and overweight during the period of martial law. Materials and methods. The study included 40 men with gastroesophageal reflux disease and overweight. The subjects were aged from 20 to 57 years, the average age was (42.8 ± 1.2) years. According to the results of esophagogastroduodenoscopy with narrow band imaging, 25 patients were diagnosed with erosive esophagitis, and 15 with non-erosive reflux disease. The serum levels of total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-С) were determined in all subjects using a Stat Fax 4500 biochemical analyzer (USA). Low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C) and atherogenic index (AI) were also calculated. Results. The average group content of TC in the blood serum of patients with erosive esophagitis was 1.2 times higher compared to the control group (p = 0.0083) due to the presence of hypercholesterolemia in 36.0 % of patients. Despite the fact that the median TG content in both groups did not exceed the reference values, hypertriglyceridemia was noted in 32 % of participants with erosive esophagitis. In addition, these patients, in contrast to the control group, had a significant decrease in HDL-C levels by 1.4 times (p = 0.0042) with a simultaneous 2-fold increase in VLDL-C (p = 0.0024) and a 1.8-fold in AI (p = 0.0069). In patients with erosive esophagitis, the ratios of TG/HDL-C, LDL-C/HDL-C and TC/HDL-C were, respectively, 1.4 times (p = 0.0246), 1.3 times (p = 0.0295) and 1.2 times (p = 0.0085) higher than in patients with non-erosive reflux disease. Patients with erosive esophagitis had direct correlations between body mass index and TG/HDL-C (r = 0.47; p = 0.0003), LDL-C/HDL-C (r = 0.34; p = 0.011) and TC/HDL-C (r = 0.44; p = 0.0007). Conclusions. The diagnostic criteria for the formation of a group at risk of developing erosive esophagitis among patients with gastroesophageal reflux disease and overweight during the period of martial law are the ratios of TG/HDL-C (sensitivity 78.6 %, specificity 87.5 %), TC/HDLC (sensitivity 82.1 %, specificity 62.5 %), LDL-C/HDL-C (sensitivity 75.0 %, specificity 56.2 %). Therefore, in case of contraindications or limited access to esophagogastroduodenoscopy, it is necessary to study lipid metabolism indicators and their correlations in patients with gastroesophageal reflux disease and overweight.
The family of Krikents: from Lithuania — to the banks of the Dnieper
Authors: M.P. Chaban, Z.I. Shevtsova, V.V. Gaponov
Journal: GASTROENTEROLOGY (2025)

The article presents the difficult life path of the representatives of the Krikent family of German origin. The head of the family, Karl Krikent, worked at a metallurgical plant in Kamianske, in the Katerynoslav-Dnipropetrovsk region. The family had four sons: Oscar, Rudolf, Alfred, and Edmund. The three older brothers devoted themselves to the medical profession, working selflessly in various medical institutions. Only the youngest, Edmund, chose the field of engineering. A separate story is presented about each of them. Special attention is paid to their descendants, in particular, Maryna Tykhomyrova (granddaughter of Oscar Krikent) who shared her local history research and family memories.
Hepatic miR-93 promotes the pathogenesis of metabolic dysfunction-associated steatotic liver disease by suppressing SIRT1
Authors: Yo Han Lee, Jinyoung Lee, Joonho Jeong, Kieun Park, Bukyung Baik, Yuseong Kwon, Kimyeong Kim, Keon Woo Khim, Haneal Ji, Ji Young Lee, Kwangho Kim, Ji Won Kim, Tam Dao, Misung Kim, Tae Young Lee, Yong Ryoul Yang, Haejin Yoon, Dongryeol Ryu, Seonghwan Hwang, Haeseung Lee, Dougu Nam, Won Kon Kim, Neung Hwa Park, Hwayoung Yun, Jang Hyun Choi
Journal: Metabolism (2025)
NIR-II AIEgen with High Photothermal Efficiency for Mild PTT: Optimized Natural Killer Cell Spatial Distribution for Boosted Immune Response
Authors: Yan Feng, Junjun Ni, Huilin Xie, Na Zhu, Wenjing Liu, Liang Guo, Jianquan Zhang, Jia Di, Shuixiang He, Hao Hu, Hui Xing, Feng Xu, Guorui Jin, Ben Zhong Tang, Xiaoran Yin
Journal: Biomaterials (2025)
Re: Philip Cornford, Roderick C.N. van den Bergh, Erik Briers, et al. EAU-EANM-ESTRO-ESUR-ISUP-SIOG Guidelines on Prostate Cancer—2024 Update. Part I: Screening, Diagnosis, and Local Treatment with Curative Intent. Eur Urol 2024;86:148–63
Authors: Andrew Vickers, Karim Touijer
Journal: European Urology (2025)
Aminated Fullerene for Comprehensive Dry Eye Therapy: Promoting Epithelial-Barrier Reconstruction and Nerve Regeneration by Suppressing Oxidation and Inflammation
Authors: Ying Lyu, Qichuan Yin, Xiaodan Liao, Youxuan Xie, Hao Yang, Yilei Cui, Yuqi Han, Ke Yao, Chunru Wang, Xingchao Shentu
Journal: Biomaterials (2025)
FS536, a novel nitric oxide-releasing doxorubicin hybrid, reverts multidrug resistance in lung cancer cells
Authors: Benedetta Romano, Maria Cristina Molaro, Fabio Somma, Chiara Battisegola, Mariacristina Failla, Loretta Lazzarato, Konstantin Chegaev, Barbara Rolando, Joanna Kopecka, Angela Ianaro, Maria Grazia Rimoli, Carminia Maria Della Corte, Chiara Riganti, Federica Sodano, Giuseppe Ercolano
Journal: Journal of Controlled Release (2025)
Tailoring the release of highly loaded amorphous solid dispersions via additive manufacturing
Authors: Carolina Alva, Elisa Goetzinger, Josip Matić, Aygün Doğan, Eyke Slama, Sarah Heupl, Thomas Rillmann, Susanna Abrahmsén-Alami, Jonathan Booth, Sharareh Salar-Behzadi, Martin Spoerk
Journal: Journal of Controlled Release (2025)
Anti-GPRC5D CAR T-cell therapy as a salvage treatment in patients with progressive multiple myeloma after anti-BCMA CAR T-cell therapy: a single-centre, single-arm, phase 2 trial
Authors: Jieyun Xia, Qian Sun, Dian Zhou, Hujun Li, Ying Wang, Yuekun Qi, Jiang Cao, Zhiling Yan, Depeng Li, Hai Cheng, Wei Sang, Feng Zhu, Haiying Sun, Wei Chen, Kunming Qi, Dongmei Yan, Tingting Qiu, Tingyu Hu, Weiying Gu, Jun Qian, Fan Xia, Na Qi, Congqian Jin, Yang Liu, Xue Wang, Yanlei Zhang, Shuixiu Peng, Zhenyu Li, Alex H Chang, Kailin Xu
Journal: The Lancet Haematology (2025)
Belzutifan for von Hippel-Lindau disease-associated renal cell carcinoma and other neoplasms (LITESPARK-004): 50 months follow-up from a single-arm, phase 2 study
Authors: Ramaprasad Srinivasan, Othon Iliopoulos, Kathryn E Beckermann, Vivek Narayan, Benjamin L Maughan, Stephane Oudard, Tobias Else, Jodi K Maranchie, Ane B Iversen, Jerry Cornell, Rodolfo F Perini, Yanfang Liu, W Marston Linehan, Eric Jonasch
Journal: The Lancet Oncology (2025)
The Quinoline Photoremovable Group (PPG) Platform—A Medicinal Chemist's Approach for Photocage Development and Applications
Authors: Bence Kontra, Zoltán Mucsi, Janez Ilaš, Petra Dunkel
Journal: Medicinal Research Reviews (2025)

Photoremovable protecting groups (PPGs) offer a straightforward solution for the temporary inactivation of biologically active substrates and their subsequent controlled release by light irradiation. Their relatively easy design and mode of application have made them useful tools for studying dynamic biological processes in vitro and in vivo. Recently, there has been a growing body of data investigating their potential application in the development of drug delivery systems. Of the various PPG scaffolds in use, quinoline photocages have a history of about 20 years. The structure-property relationships of quinoline PPGs, as well as alternative multibranch designs based on quinoline monomers have been thoroughly studied both experimentally and theoretically. Therefore, quinoline PPGs serve as a representative study of PPG development, showing how the various applications of quinoline photocages followed the chemical optimization or how the applications drove the chemical design. Since the raison d'être of PPGs lies in their application for light-activated release of various substrates or performing light-activated structural changes in materials, it is crucial to understand how PPGs are selected and utilized by their end-users, who are often not chemists themselves. Therefore, we discuss whether the conclusions drawn from the selected quinoline PPG family could lead to more general insights for the field as whole. As PPG-related applications still rely heavily on a limited number of chemical scaffolds, it is worth considering, what could be the reasons for the slow uptake of novel chemical scaffolds.
PlateletBase: A Comprehensive Knowledgebase for Platelet Research and Disease Insights
Authors: Huaichao Luo, Changchun Wu, Sisi Yu, Hanxiao Ren, Xing Yin, Ruiling Zu, Lubei Rao, Peiying Zhang, Xingmei Zhang, Ruohao Wu, Ping Leng, Kaijiong Zhang, Qi Peng, Bangrong Cao, Rui Qin, Hulin Wei, Jianlin Qiao, Shanling Xu, Qun Yi, Yang Zhang, Jian Huang, Dongsheng Wang
Journal: Genomics Proteomics & Bioinformatics (2025)