tbuckley/cabot-search huggingface dataset:
Inhalable Dry Powders for Lung mRNA Delivery
Authors: Akiva Gordon, Bowen Li, Jacob Witten, Hong Nguyen, Daniel G. Anderson
Journal: Advanced Healthcare Materials (2024)

Abstract Despite great promise, application of mRNA therapeutics in the lung has proven challenging. Many groups have reported success instilling liquid mRNA formulations in animal models, but direct intratracheal administration of large liquid quantities to the human lung presents significant safety and distribution concerns. To accomplish safe and effective mRNA delivery to the lung, formulations must be prepared for dosing via inhalation. An inhaled mRNA delivery system for the lung must be both robust enough to survive inhalation conditions and potent enough to deliver mRNA upon reaching the lung. In this work dry powder lipid nanoparticle formulations are developed, using spray‐freeze‐drying, to produce stable, biologically active, inhalable dry powders for mRNA delivery. The final powders have suitable aerosolization properties, with mean mass aerodynamic diameter (MMAD) of 3–4 microns, and fine particle fraction (FPF) ≈40%, allowing for efficient mRNA delivery to the deep lung following inhalation. Importantly, the formulations developed here are suitable for use with different ionizable lipids. Four different ionizable lipid‐based formulations are evaluated as powders, and all exhibit in vivo pulmonary mRNA delivery equal to that of instilled liquid formulations. These results lay promising groundwork for the eventual development of an inhalable mRNA dry powder therapeutic.
Breast cancer statistics 2024
Authors: Angela N. Giaquinto, Hyuna Sung, Lisa A. Newman, Rachel A. Freedman, Robert A. Smith, Jessica Star, Ahmedin Jemal, Rebecca L. Siegel
Journal: CA A Cancer Journal for Clinicians (2024)

Abstract This is the American Cancer Society's biennial update of statistics on breast cancer among women based on high‐quality incidence and mortality data from the National Cancer Institute and the Centers for Disease Control and Prevention. Breast cancer incidence continued an upward trend, rising by 1% annually during 2012–2021, largely confined to localized‐stage and hormone receptor‐positive disease. A steeper increase in women younger than 50 years (1.4% annually) versus 50 years and older (0.7%) overall was only significant among White women. Asian American/Pacific Islander women had the fastest increase in both age groups (2.7% and 2.5% per year, respectively); consequently, young Asian American/Pacific Islander women had the second lowest rate in 2000 (57.4 per 100,000) but the highest rate in 2021 (86.3 per 100,000) alongside White women (86.4 per 100,000), surpassing Black women (81.5 per 100,000). In contrast, the overall breast cancer death rate continuously declined during 1989–2022 by 44% overall, translating to 517,900 fewer breast cancer deaths during this time. However, not all women have experienced this progress; mortality remained unchanged since 1990 in American Indian/Alaska Native women, and Black women have 38% higher mortality than White women despite 5% lower incidence. Although the Black‐White disparity partly reflects more triple‐negative cancers, Black women have the lowest survival for every breast cancer subtype and stage except localized disease, with which they are 10% less likely to be diagnosed than White women (58% vs. 68%), highlighting disadvantages in social determinants of health. Progress against breast cancer could be accelerated by mitigating racial, ethnic, and social disparities through improved clinical trial representation and access to high‐quality screening and treatment.
Sticky Science: Using Complex Coacervate Adhesives for Biomedical Applications
Authors: Ayla N. Kwant, Julien S. Es Sayed, Marleen Kamperman, Janette K. Burgess, Dirk‐Jan Slebos, Simon D. Pouwels
Journal: Advanced Healthcare Materials (2024)

Abstract Tissue adhesives are used for various medical applications, including wound closure, bleeding control, and bone healing. Currently available options often show weak adhesion or cause adverse effects. Recently, there has been an increasing interest in complex coacervates as medical adhesives. Complex coacervates are formed by mixing oppositely charged macromolecules that associate and undergo liquid‐liquid phase separation, in which the dense bottom phase is the complex coacervate. Complex coacervates are strong and often biocompatible, and show strong underwater adhesion. The properties of the resulting materials are tunable by intrinsic factors such as polymer chemistry, molecular weight, charge density, and topology of the macromolecules, as well as extrinsic factors such as temperature, pH, and salt concentration. Therefore, complex coacervates are interesting new candidates for medical adhesives. In this review, it is described how complex coacervates form and how different factors influence their behavior. Next, an overview of recent studies on complex coacervates in the context of medical adhesives is presented. The application of complex coacervates as hemostatic or embolic agents, skin or bone repair adhesives, and soft tissue sealants is discussed. Lastly, additional possibilities for utilizing these materials in the future are discussed.
The prognosis of patients with locally advanced cervical cancer undergoing surgical versus non-surgical treatment: A retrospective cohort study based on SEER database and a single-center data
Authors: Xinyan Gao, Yan Kong, Ying Ning, Tian Tian, Xiaojing Gai, Ke Lei, Zuimei Cui
Journal: International Journal of Surgery (2024)

The aim of this study was to investigate the impact of surgical treatment on the survival prognosis of patients with locally advanced cervical cancer (LACC) and to identify factors that may influence the efficacy of surgery. Data from the SEER database (2000–2020) and a hospital (2013–2023) were collected for this investigation. Utilizing multivariable Cox regression analysis, Kaplan-Meier survival analysis, and log-rank tests, we assessed the effects of surgical intervention on overall survival (OS) and disease-specific survival (DSS) in LACC patients. Our results revealed that in the SEER database, the surgical group exhibited significantly better OS and DSS compared to the non-surgical group. Particularly noteworthy was the significantly higher survival rate in the surgical group for patients with tumor diameters less than 6 cm. Furthermore, both OS and DSS were improved in the surgical group regardless of whether the cancer was squamous cell carcinoma or adenocarcinoma. Additionally, patients who underwent surgery combined with radiotherapy had notably better OS and DSS compared to those who received chemoradiotherapy alone. Similarly, our hospital data showed that the surgical group demonstrated significantly better OS than the non-surgical group, especially for patients with tumors smaller than 6 cm in diameter. These findings suggest that surgery combined with radiotherapy may offer more favorable outcomes than chemoradiotherapy alone, particularly for LACC patients with smaller tumors.
Multi-site DNA methylation alterations of peripheral blood mononuclear cells serve as novel biomarkers for the diagnosis of AIS/stage I lung adenocarcinoma: A multi-center cohort study
Authors: Peilong Li, Shibiao Liu, Tiantian Wang, Fang Wang, Juan Li, Qiuchen Qi, Shujun Zhang, Yan Xie, Jianping Li, Yongcai Zhu, Suli Yang, Guotao Yin, Xiaoyi He, Shijun Li, Huiting Xu, Mengqiu Xiong, Guanghua Li, Yi Zhang, Lutao Du, Chuanxin Wang
Journal: International Journal of Surgery (2024)

Background: Early diagnosis remains an obstacle for improving the outcome of lung adenocarcinoma (LUAD). DNA methylation changes in peripheral blood mononuclear cells (PBMCs) could reflect immune response to tumorigenesis, providing the theoretical basis for early cancer diagnosis based on immune cell profiling. Methods: This multi-center study evaluated the DNA methylation patterns based on PBMCs samples from 1115 individuals at nine medical centers. Genome-wide DNA methylation profiling of PBMCs in a discovery cohort (35 LUAD patients and 50 healthy controls) was performed using Illumina 850K microarray. Candidate differentially methylated CpG positions (DMPs) were selected and validated in a two-step DMPs screening cohort (65 LUAD patients and 80 healthy controls) by pyrosequencing and multiple target region methylation enrichment sequencing (MTRMES). Then, an early LUAD Diagnostic Panel (LDP score) based on multi-site methylation-specific chip-based digital PCR was constructed in a training set and then confirmed in a validation set from the LDP score development cohort (389 AIS/stage I LUAD patients and 293 healthy controls). Besides, we included 157 other cancer patients, including 52 gastric cancer (GC) patients, 50 breast cancer (BC) patients, and 55 colorectal cancer (CRC) patients to assess the specificity of LDP score. In addition, we also evaluated the early warning ability of LDP score for LUAD in a prospective cohort (46 people who were at high-risk of developing LC). Results: A total of 1415 LUAD-specific DMPs were identified. Then, six DMPs were selected for validation and three DMPs were finally verified. The LDP score was constructed by combining the three DMPs, age, and gender, and showed an AUC of 0.916, sensitivity of 88.17%, and specificity of 80.20% in combined set, outperforming traditional methods, such as CEA and CT (detection rate: 87.79% vs. 4.69%; 87.79% vs. 35.21%). This diagnostic performance was confirmed in sub-types of LUAD with clinical challenges, such as 6-20 mm LUAD (AUC: 0.914, 95%CI: 0.889-0.934) and ground-glass nodules (AUC: 0.916, 95%CI: 0.889-0.938). Importantly, our LDP score had significant improvement in terms of selecting high-risk individuals who should receive low-dose computed tomography (87.80% vs. 9.28%). Remarkably, LDP score could predict LUAD around two years before clinical diagnosis in our prospective cohort. Conclusions: The novel developed LDP score represented a convenient and effective assay for the detection of AIS/stage I LUAD with high sensitivity and specificity, and had demonstrated unique advantages over traditional detection methods.
Mapping the evolution of 3D printing in cardio-thoracic diseases: a global bibliometric analysis
Authors: Jie Tian, Ying-Xian Dong, Li Wang, Yong-Ming Wu, Zi-Yi Zhao, Guo-Wei Che
Journal: International Journal of Surgery (2024)

Background: Despite the growing research on 3D printing (3DP) in cardio-thoracic diseases, comprehensive bibliometric analyses remain scarce. This study aims to bridge this gap by identifying key research trends and hotspots within the field. Methods: A bibliometric analysis was conducted on publications from 1991 to 2024 using data from the Web of Science Core Collection, with analysis performed using VOSviewer, CiteSpace, and the R package ‘bibliometrix’. Results: The analysis included 2,836 documents authored by 14,206 researchers across 85 countries. A significant rise in annual publications was observed, with the United States, China, and the United Kingdom leading in contributions. Prominent institutions, including Stanford University, were highlighted, while Scientific Reports and Biomaterials were identified as influential journals. Key research areas encompass cardiovascular, lung, and breast diseases, along with chest wall reconstructions, with emerging trends focusing on advanced materials for drug delivery and tissue engineering. Conclusion: This comprehensive bibliometric analysis of 3DP in cardio-thoracic diseases reveals global research trends, emerging themes, and the crucial role of 3DP in advancing medical education and personalized treatment, highlighting areas for future research and development.
Clinical utility of extracorporeal shock wave therapy in restoring hand function of patients with nerve injury and hypertrophic scars due to burns: A prospective, randomized, double-blinded study
Authors: Seung Yeol Lee, Yoon Soo Cho, Cheong Hoon Seo, Jisu Seo, So Young Joo
Journal: International Journal of Surgery (2024)

Background: Joint contractures and nerve injuries are common after hand burns. Extracorporeal shock wave therapy (ESWT) is effective not only for the regeneration of various tissues, including scar tissues, but also for reducing pain and pruritus in patients with burns. Researchers have attempted to explore the effects of ESWT on hand dysfunction caused by nerve injury following burns. Materials and methods: We evaluated the effects of ESWT (compared to sham stimulation) on hands with nerve injury and hypertrophic scars and thereby on hand function. The current study was a double-blind randomized controlled trial involving 120 patients. The ESWT parameters were as follows: energy flux density, 0.05–0.30 mJ/mm 2 ; frequency, 4 Hz; 1000 to 3000 impulses per treatment; and 12 treatments, one/week for 12 weeks. Outcome measures were as follows: 10-point visual analog scale for pain, Jebsen-Taylor hand function test, grip strength, Purdue Pegboard test, ultrasound measurement of scar thickness, and skin characteristics before and immediately after 12 weeks of treatment. Results: No significant inter-group difference was noted after the initial evaluation ( P &gt;0.05). More significant improvements were found in the ESWT group than in the sham group in terms of the VAS score ( P =0.004), extension ROMs of hand joints ( P =0.02), the JTT scores (writing, small, and light) ( P &lt;0.001, P &lt;0.001, and P =0.002), and skin characteristics (melanin, skin distensibility, and biologic skin elasticity) ( P =0.004, P &lt;0.001, and P &lt;0.001). Other measured outcomes did not differ between the two groups after the treatment. Conclusion: We identified the clinically beneficial effects of ESWT in promoting hand function, improving scarring, and alleviating scarring-related pain, thereby highlighting its advantages in improving hand function that has been impaired due to nerve injury and hypertrophic scars after burns.
In-depth cerebrovascular lipidomics profiling for discovering novel biomarkers and mechanisms in moyamoya and intracranial atherosclerotic disease
Authors: Kangmin He, Xinmei Wang, Yu Gu, Xiao Tong, Xuanfeng Qin, Yujun Liao, Li-Hao Huang, Jiaxi Wang, Bin Xu
Journal: International Journal of Surgery (2024)

Background: Despite considerable research efforts, the precise etiology and underlying pathways contributing to Moyamoya Disease (MMD) remain poorly understood. Moreover, the overlapping vascular pathologies shared between MMD and Intracranial Atherosclerotic Disease (ICAD) pose challenges in clinical differentiation, even with gold-standard cerebral angiography. An in-depth exploration of lipidomic alterations in cerebral intracranial MMD vessels could offer valuable insights into the pathogenesis of MMD-related mechanisms, encompassing MMD and ICAD, and unveil novel biomarkers and potential therapeutic targets. However, to date, comprehensive lipidomic profiling has been lacking. Materials and Methods: To discover novel biomarkers and unravel the pathophysiological mechanisms underlying MMD, we conducted a lipidomics analysis to characterize various lipid species in matched human extracranial and intracranial artery tissues from patients diagnosed with MMD (n=99) and ICAD (n=12). Results: Our analysis identified 569 lipid species and delineated a robust panel of lipidomic biomarkers capable of effectively distinguishing MMD from ICAD (area under curve=0.98), as determined by receiver operating characteristic curve analysis. Notably, we observed a significantly more pronounced positive correlation of diacylglycerols and a negative association of triglycerides in intracranial artery tissues of MMD patients compared to those with ICAD, suggesting a potential role of dysregulated diacylglycerol-induced signaling in MMD pathogenesis. Furthermore, our investigation into the correlations of critical differential intracranial artery vessel lipid species between MMD and ICAD and clinical parameters revealed negative associations with plasma iron levels, implying a potential link between plasma iron metabolism and artery lipid homeostasis during MMD pathogenesis. Conclusion: These findings offer promising prospects for advancing clinical diagnosis for enhanced differentiation between the two disease conditions. Additionally, they shed light on the fundamental mechanisms implicated in MMD pathogenesis and suggest potential therapeutic avenues through targeting artery vessel lipids or plasma iron levels.
Three-dimensional adipose-derived stem cell spheroids enhance adipogenesis and angiogenesis in fat grafts: Experimental research
Authors: Sang-Oh Lee, Bo-Yoon Park, Tiep Tien Nguyen, Kyoo-Ri Kwon, Mi-Jin Kim, Hae Young Yang, Jay Kim, Jee-Heon Jeong, Il-Kug Kim
Journal: International Journal of Surgery (2024)
Elastography-based AI model can predict axillary status after neoadjuvant chemotherapy in breast cancer with nodal involvement: A prospective, multicenter, diagnostic study
Authors: Jia-Xin Huang, Yao Lu, Yu-Ting Tan, Feng-Tao Liu, Yi-Liang Li, Xue-Yan Wang, Jia-Hui Huang, Shi-Yang Lin, Gui-Ling Huang, Yu-Ting Zhang, Xiao-Qing Pei
Journal: International Journal of Surgery (2024)

Objective: To develop a model for accurate prediction of axillary lymph node (LN) status after neoadjuvant chemotherapy (NAC) in breast cancer patients with nodal involvement. Methods: Between October 2018 and February 2024, 671 breast cancer patients with biopsy-proven LN metastasis who received NAC followed by axillary LN dissection were enrolled in this prospective, multicenter study. Preoperative ultrasound (US) images, including B-mode ultrasound (BUS) and shear wave elastography (SWE), were obtained. The included patients were randomly divided at a ratio of 8:2 into a training set and an independent test set, with five-fold cross-validation applied to training set. We first identified clinicopathological characteristics and conventional US features significantly associated with the axillary LN response and developed corresponding prediction models. We then constructed deep learning radiomics (DLR) models based on BUS and SWE data. Models performances were compared, and a combination model was developed using significant clinicopathological data and interpreted US features with the SWE-based DLR model. Discrimination, calibration and clinical utility of this model were analyzed using receiver operating characteristic curve, calibration curve and decision curve, respectively. Results: Axillary pathologic complete response (pCR) was achieved in 52.41% of patients. In the test cohort, the clinicopathologic model had an accuracy of 71.30%, while radiologists’ diagnoses ranged from 64.26% to 71.11%, indicating limited to moderate predictive ability for the axillary response to NAC. The SWE-based DLR model, with an accuracy of 80.81%, significantly outperformed the BUS-based DLR model, which scored 59.57%. The combination DLR model boasted an accuracy of 88.70% and a false-negative rate of 8.82%. It demonstrated strong discriminatory ability (AUC, 0.95), precise calibration ( p value obtained by Hosmer–Lemeshow goodness-of-fit test, 0.68), and practical clinical utility (probability threshold, 2.5-97.5%). Conclusions: The combination SWE-based DLR model can predict the axillary status after NAC in patients with node-positive breast cancer, and thus, may inform clinical decision-making to help avoid unnecessary axillary LN dissection.
Inhalable and bioactive lipid-nanomedicine based on bergapten for targeted acute lung injury therapy via orchestrating macrophage polarization
Authors: Ran Liao, Zhi-Chao Sun, Liying Wang, Caihong Xian, Ran Lin, Guifeng Zhuo, Haiyan Wang, Yifei Fang, Yuntao Liu, Rongyuan Yang, Jun Wu, Zhongde Zhang
Journal: Bioactive Materials (2024)
RRFERV stabilizes TEAD1 expression to mediate nasopharyngeal cancer radiation resistance rendering tumor cells vulnerable to ferroptosis
Authors: Qingqing Xu, Xin Wen, Chenglong Huang, Zaishan Lin, Zhen Xu, Ciming Sun, Li Li, Suixian Zhang, Shuanghong Song, Jiahao Lou, Zan Hou, Yuanyuan Chen, Xuan Li, Lei Chen
Journal: International Journal of Surgery (2024)
Safety and efficacy of stem cell therapy for Crohn’s disease: An umbrella review of systematic reviews
Authors: Lakshmi Thangavelu, Syam Mohan, Hassan Ahmad Alfaifi, Abdullah Farasani, Soumya V. Menon, Pooja Bansal, Chhavi Choudhary, M Ravi Kumar, Raghav Vashishth, Afrah Majeed Ahmed Al- Rihaymee, Sarvesh Rustagi, Anil K. Malhotra, Muhammed Shabil, Mahalaqua Nazli Khatib, Quazi Syed Zahiruddin, Siddig Ibrahim Abdelwahab, Ganesh Bushi, Saleh Hussain A. Almasabi, Hayam A. Alrasheed, Ali A. Rabaan
Journal: International Journal of Surgery (2024)
Residential exposure to transportation noise and risk of incident atrial fibrillation: a pooled study of 11 prospective Nordic cohorts
Authors: Jesse D Thacher, Nina Roswall, Mikael Ögren, Andrei Pyko, Agneta Åkesson, Anna Oudin, Annika Rosengren, Aslak H Poulsen, Charlotta Eriksson, David Segersson, Debora Rizzuto, Emilie Helte, Eva M Andersson, Gunn Marit Aasvang, Gunnar Engström, Hrafnhildur Gudjonsdottir, Jenny Selander, Jesper H Christensen, Jørgen Brandt, Karin Leander, Kim Overvad, Kristoffer Mattisson, Kristina Eneroth, Lara Stucki, Lars Barregard, Leo Stockfelt, Maria Albin, Mette K Simonsen, Ole Raaschou-Nielsen, Pekka Jousilahti, Pekka Tiittanen, Petter L S Ljungman, Steen S Jensen, Susanna Gustafsson, Tarja Yli-Tuomi, Thomas Cole-Hunter, Timo Lanki, Youn-Hee Lim, Zorana J Andersen, Göran Pershagen, Mette Sørensen
Journal: The Lancet Regional Health - Europe (2024)
Hydrogel microsphere stem cell encapsulation enhances cardiomyocyte differentiation and functionality in scalable suspension system
Authors: Mohammadjafar Hashemi, Ferdous B Finklea, Hanna Hammons, Yuan Tian, Nathan Young, Emma Kim, Caroline Halloin, Wiebke Triebert, Robert Zweigerdt, Amit Kumar Mitra, Elizabeth A Lipke
Journal: Bioactive Materials (2024)
Food Retention at Endoscopy Among Adults Using Glucagon-Like Peptide-1 Receptor Agonists
Authors: Jason Nasser, Ava Hosseini, Gillian Barlow, Roma Gianchandani, Ali Rezaie, Mark Pimentel, Ruchi Mathur
Journal: JAMA Network Open (2024)

This cross-sectional study investigates the association between glucagon-like peptide-1 receptor agonists and food retention during esophagogastroduodenoscopy.
Temporary FDA Advisory Committee Members, Recommendations, and Agency Actions, 2017-2021
Authors: Nikhil Chaudhry, Audrey D. Zhang, Jason L. Schwartz, Reshma Ramachandran, Joseph S. Ross
Journal: JAMA Network Open (2024)

This cross-sectional study examines the recommendations and agency actions of temporary and permanent US Food and Drug Administration advisory committee members from 2017 to 2021.