tbuckley/cabot-search huggingface dataset:
Abstract 692: Class II HDACs Are Required During Early Stage Mouse Embryonic Stem Cell Differentiation in Response to Nitric Oxide
Authors: Barbara Illi, Francesco Spallotta, Jessica Rosati, Stefania Mattiussi, Simona Nanni, Dante Rotili, Sergio Valente, Antonello Mai, Antonella Farsetti, Maurizio C Capogrossi, Carlo Gaetano
Journal: Circulation (2007)

INTRODUCTION &amp; BACKGROUND: We have recently described that in mouse embryonic stem cells (ES) laminar shear stress provides chromatin programming signals. This effect is similar to that of nitric oxide (NO) which directly promotes cardiomyogenesis underlying the possible presence of a common mechanism of action the characterization of which has been the object of the present study. METHODS &amp; RESULTS: ES were treated with the NO donor DETANO from 1 to 24 hours (h) in the presence of absence of leukaemia inhibitory factor (LIF). In LIF-deprived cells the NO donor determined a marked global reduction in histone H3 acetylation paralleled by the expression of a number of cardiovascular markers. In this condition the stemness-associated gene product Oct4 decreased rapidly at mRNA and protein level underlying the potential opening of a NO-dependent differentiation program. Further class-II histone deacetylase (HDAC) 4, 5, and 7, rapidly shuttled to nucleus upon LIF deprivation. This phenomenon was transient as HDAC molecules returned to the cytoplasm in about 6 h. Intriguingly, the exposure of ES to the NO donor allowed the nuclear retention of these enzymes still detectable at the 6 h time-point. Further, we found that NO treatment stimulated the formation of nuclear complexes including the class II HDACs 4, 5 and the protein phosphatase 2A. Remarkably, ES treatment with the class II selective inhibitor MC 1568 prevented ES differentiation in the presence of NO. CONCLUSIONS: These results suggest a potential role for class II HDACs in the NO-dependent early stage of ES commitment upon LIF removal.
Effect of Testosterone Propionate on the Adrenals and on the Incidence of Mammary Cancer in the RIII Strain of Mice
Authors: Jacob Heiman
Journal: Cancer Research (1944)

The hormonal relationship that Cramer and Horning (6, 8, 9) believe to exist between brown degeneration of the adrenals and carcinoma of the mammae in high tumor strain mice has been questioned by other observers, but not definitely disproved. Details may be found in the papers of Dobrovolska~a-Zavadskaia and her group (15-17); Lacassagne and Raynaud (25, 26); Blaisdell, Gardner, and Strong (1); Bonser (3); and Kreyberg (23). This degeneration occurs frequently at an early age in female mice of a high tumor strain, according to Cramer (8) and Dobrovolskai'a-Zavadskai'a (17), but is seen also in the tumorfree males of the same strain at a later age. It has been described by Whitehead (31), Martin (28), Howard-Miller (21 ), Burrows (5), Daughady (11), and Deanesly (12) as seen at the cortico-medullary junction, in the periphery of the medulla, or in the medial part of the zona reticularis. The change significantly begins in the x-zone, which, according to Howard-Miller, is A transitory zone showing age and sex relationships in  The appearance of brown degeneration around the x-zone in various strains of mice after estrinization as described by Burrows (4, 5), Cramer (6), and Martin (28), and the induction of mammary tumors in similar strains also by estrinization reported by Cramer (10), Bonser and her associates (2), Lacassagne (24), Twombly (30), and Gardner (18) warranted further studies on this apparent relationship. Burrows (5) states that the effect of castration, pregnancy, and estrogen on the presence or absence of the x-zone suggests that it may be influenced by testosterone or progesterone, and notes that these hormones seem to hasten the involution or absorption that normally occurs in the x-zone of adrenals in female mice. Deanesly points out that castration of the male causes reappearance of the x-zone, converting the male adrenal to the female type. Woolley, Fekete, and Little (32) have observed nodular hyperplasia and carcinoma of the adrenals in ovariectomized mice that show a return of estrogen effect. Martin (28) noted the dis-
Abstract 1367: Altered Transmural Expression Of I <sub>Kr</sub> In Cardiac Memory Is Regulated By The Transcription Factor, Activator Protein-1
Authors: Nazira Ozgen, Alexei N Plotnikov, Iryna N Shlapakova, Peter Danilo, Michael R Rosen
Journal: Circulation (2007)

Introduction: We previously reported that the transient outward current (I to ) and mRNA and protein levels of its accessory subunit KChIP2 decrease in ventricular pacing-induced cardiac memory (CM). These changes appear dependent on a decrease of the cAMP response element binding protein (CREB). We have also reported that the transmural gradients of the delayed rectifier potassium current component, I kr and the mRNA and protein of its subunit ERG (all showing epicardium (EPI) &gt; endocardium (ENDO)), are diminished in CM. However, there is no CREB binding site in the ERG promoter region to explain the change in ERG. We now tested the hypothesis that 1) the normal transmural gradient of ERG expression in canine left ventricle (LV) is paralleled by a transmural gradient of the Activator protein-1 (AP-1) components c-Jun and c-Fos, and 2) altered transmural expression of c-Jun and c-Fos parallels the altered transmural ERG in CM. Methods: We screened 2000bp upstream from the first exon of ERG for transcription factor binding sites. Then 10 dogs were chronically instrumented with LV posterior wall and LA pacing electrodes. Five underwent 3w AV-sequential pacing (CM), and 5, 3w of atrial pacing (Sham). CM was quantified as change in T wave vector displacement (TVD). At 3w biopsies were taken 1–2 cm from the LV pacing site. Real time PCR was used to determine c-Jun and c-Fos. Results: Screening revealed an AP-1 response element upstream to the ERG promoter region, supporting the hypothesis that c-Jun and c-Fos might be reasonable candidate transcription factors here. TVD increased in CM compared to Sham. As previously, mRNA and protein transmural gradients of ERG decreased in CM. C-jun and c-Fos exhibited transmural gradients, EPI&gt;ENDO, in Sham (Table ). This gradient was lost in CM. Conclusion AP-1 is a transcription factor for ERG and the altered ERG transmural gradient in CM may be regulated by the altered c-Jun and c-Fos gradient. T vector displacement (TVD) and mRNA of c-Jun and c-Fos
Phencyclidine-induced psychosis
Authors: R M Allen, S J Young
Journal: American Journal of Psychiatry (1978)

During a 13-month period, 9 patients with phencyclidine-induced psychosis were admitted to Darnall Army Hospital. They exhibited hostility agitation, and tangentiality and had delusions of influence and religious grandiosity. Six subjects reported auditory hallucinations, and 4 were disoriented in at least 1 sphere. Despite treatment with antipsychotic medication, the psychotic episodes often persisted for more than 30 days. Our clinical finding of prolonged psychotic reactions, together with previous reports of the effects of phencyclidine, suggests that phenycyclidine provides an intriguing drug model for schizophrenia.
Abstract 67: Vasopressin impairs Brain, Heart and Kidney Perfusion in Acute Heart Failure
Authors: Stig Müller, Ole-Jakob How, Stig E Hermansen, Truls Myrmel
Journal: Circulation (2007)

Arginin Vasopressin (AVP) is increasingly used to restore mean arterial pressure (MAP) in various circulatory shock states including cardiogenic shock. This is potentially deleterious since AVP is also known to reduce cardiac output by increasing vascular resistance. Aim: We hypothesized that restoring MAP by AVP improves vital organ blood flow in experimental acute cardiac failure. Methods: Cardiac output (CO) and arterial blood flow to the brain, heart, kidney and liver were measured in nine pigs by transit-time flow probes. Heart function and contractility were measured using left ventricular Pressure-Volume catheters. Catheters in central arteries and veins were used for pressure recordings and blood sampling. Left ventricular dysfunction was induced by intermittent coronary occlusions, inducing an 18 % reduction in cardiac output and a drop in MAP from 87 ± 3 to 67 ± 4 mmHg. Results: A low-dose therapeutic infusion of AVP (0.005 u/kg/min) restored MAP but further impaired systemic perfusion (CO and blood flow to the brain, heart and kidney reduced by 29, 18, 23 and 34 %, respectively). The reduced blood flow was due to a 2.0, 2.2, 1.9 and 2.1 fold increase in systemic, brain, heart and kidney specific vascular resistances, respectively. Contractility remained unaffected by AVP. The hypoperfusion induced by AVP was most likely responsible for observed elevated plasma lactate levels and an increased systemic oxygen extraction. Oxygen saturation in blood drawn from the great cardiac vein fell from 31 ± 1 to 22 ± 3 % dropping as low as 10 % in one pig. Finally, these effects were reversed forty minutes after weaning the pigs form the drug. Conclusion: The pronounced reduction in coronary blood flow point to a potentially deleterious effect in postoperative cardiac surgical patients and in patients with coronary heart disease. Also, this is the first study to report a reduced cerebral perfusion by AVP.
Abstract 1870: A Novel Method of Spatially-Dense 3D Speckle Tracking on 4D Echocardiography for Strain Analysis: with Validation by Sonomicrometry
Authors: Xubo Song, Andriy Myronenko, Muhammad Ashraf, Robert I Lowe, Petra S Niemann, Ling Hui, Xiao Kui Li, Karl Thiele, David J Sahn
Journal: Circulation (2007)

Background: We have developed a method for spatially-dense 3D speckle tracking that tracks speckle features in myocardium to allow calculation of full 3D displacement and strain from 4D full-volume echo images and have tested its accuracy compared to sonomicrometry in open-chest pigs. Methods: The 4D images were acquired using a Philips iE33 on 6 open-chest piglets. An array of 6 sonomicrometers (sono crystals) was implanted as 2 triangular sets at apex and mid-levels in the LV myocardium. The pair-wise crystal distances were computed offline using Sonometrics® software to yield 3D displacements. For 4D computation of speckle based mechanics, Cartesian coordinate images were entered into an envelope detection program, then a volume-based non-rigid registration was used to obtain optimal displacement between end diastolic (ED) and end systolic (ES) volumes. The displacement field was modeled using B-splines, parameterized by the location of a set of control points. Optimal locations of the control points were obtained by minimizing the sum of squared intensity differences between the ED and ES volumes. Sono crystals were located based on anatomic markers in the ED volume before the adjacent speckle patches were tracked, to ensure that image-derived strains were computed for segments matching the distances between sono crystal pairs. The method was implemented in MATLAB. Results: The comparison of the systolic strains from dense 4D speckle tracking showed a strong correlation (r 2 = 0.82) with sonomicrometry for 44 segmental strains. Conclusions: These studies show that the method we have developed can accurately compute 3D displacement and segmented LV strain from 4D data.
Anticholinergic exacerbation of phenothiazine-induced extrapyramidal syndrome
Authors: M Alpert, F Diamond, E M Laski
Journal: American Journal of Psychiatry (1976)

The authors used tremography to record the bilateral digital tremor of a patient showing extrapyramidal system effects in response to withdrawal from phenothiazines. They found that changes in the amplitude of tremor correlated with clinical ratings of extrapyramidal disturbance. Changes in the amplitude and in the spectrum of tremor were similar to the bipolar paradoxical changes seen during intoxication with an anticholinergic psychotogen.
Patient and physician quality of life assessment in a gynecologic oncology clinic
Authors: B. E. Miller, E. Dunn, S. Danhauer, J. Lovato, R. McQuellon
Journal: Journal of Clinical Oncology (2005)

8251 Background: Accurate quality of life (QOL) assessment is an important consideration during and after cancer treatment. Patient and physician assessments are known to differ. We determined effectiveness of the systematic use of questionnaires at patient visits. Methods: A convenience sample of consecutive patients was assessed during (n=58) and after (n=43) chemotherapy. At visit 1 performance status (PS, Karnofsky Scale) and global QOL assessment with a hundred millimeter scale (UNISC) were used. At visit 2 and 3 the PS, UNISC, Functional Assessment of Cancer Therapy-G (FACT) and Center for Epidemiologic Studies-Depression (CES-D) were administered. A questionnaire regarding patient satisfaction was completed each time. The physician assessed patient PS and UNISC after each visit. Spearman correlation coefficients were used for statistical evaluation. Results: At visit 1 assessment of PS by the physician was identical to the patient in 63%, one grade higher in 20% and one grade lower in 17%; QOL assessed by the physician with the UNISC was within 10 points of the patient assessment in 50% and within 20 points in 78%. Results were similar after visits 2 and 3. Patients reported excellent satisfaction with their office visit at each time with an average score of 51 out of 55 points. Answering the questionnaires helped them think about their life (83%); helped them talk to the physician (67%) and gave their physician a better understanding of their situation (88%). More patients reported depressive symptoms (CES-D score > 17), during chemotherapy (50%) than during follow up (35%). The UNISC correlated highly with the physical, functional and overall FACT score. It took an average time of 8 minutes to complete the questionnaires. Conclusion: Systematic QOL assessment and depression screening can be efficiently delivered during a routine clinic visit. This adds and may clarify patient QOL and psychological status beyond the office visit interview alone. Single item measures may be the most useful in this situation and can be followed by more extensive assessment as necessary. No significant financial relationships to disclose.
Abstract 354: Nitric Oxide Regulates Chromatin Folding in Human Endothelial Cells via PP2A activation and HDAC-4 Nuclear Shuttling.
Authors: Barbara Illi, Claudia Colussi, Fabio Martelli, Maurizio C Capogrossi, Carlo Gaetano
Journal: Circulation (2006)

Introduction and Background: Laminar shear stress (SS) induces histone H3 and H4 modifications in human umbilical vein endothelial cells (HUVEC). This effect occurs within two hours of SS treatment...
Expectations and outcomes for patients given mental health care or spiritist healing in Puerto Rico
Authors: J D Koss
Journal: American Journal of Psychiatry (1987)

In Puerto Rico, spiritism offers a traditional alternative to community mental health services. The author compares reported expectations and outcomes of mental health center patients and patients of spiritist healers. The spiritists' patients reported significantly higher expectations, especially for mood and feeling complaints. Both patient groups had a similar duration and severity of symptoms. The outcome ratings of spiritists' patients were significantly better than those of therapists', but this difference could be accounted for by the higher expectations of the spiritists' patients. With these exceptions, the findings do not account for the selection of one type of treatment over the other.
Preserved language skills subsequent to risk-adapted treatment for ependymoma
Authors: Fiona M. Lewis, Bruce E. Murdoch
Journal: Neuro-Oncology (2011)

Background and aims: Factors that increase the risk of reduced neurocognitive outcomes for children following treatment for CNS tumours include treatment protocol, age at diagnosis, presence and duration of hydrocephaly, time post-treatment, and tumour location. The aim of the study was to assess the language outcomes of a child presenting with a heightened risk of reduced language outcomes following risk-adapted treatment at the age of 4 years 8 months for ependymoma located on the cerebellar midline. Method: Baseline assessment of general language skills and more complex higher-level language skills was undertaken at the age of 7 years 7 months. Follow-up language assessment was undertaken 18 months later. Change in performance scores over time was statistically compared to the change over 18 months in a control group of children. Results: The results of a modified t test indicated that the child's score differences between initial and subsequent assessment were not significantly different to the mean score differences of the control group. Conclusion: Despite a heightened risk of reduced language outcomes due to factors including a tumour located midline on the cerebellum and extending to the right cerebellar hemisphere, young age at diagnosis, length of time post-diagnosis at follow-up language assessment, and treatment including surgery and cranial irradiation, the child presented with strong stable language skills. Factors that may have contributed to the sparing of her language abilities include the application of risk-adapted treatment and the absence of hydrocephaly.
培養肝細胞における細胞毒性ケノデオキシコール酸(CDCA)とウルソデオキシコール酸(UDCA)およびポリエンホスファチジルコリン(PPC)とプロスタグランジンE1による細胞保護効果
Authors: Tanikawa K, Sakisaka S, Nagata E, Abe H
Journal: Hepatology (1984)
Abstract 8851: Intracoronary Infusion of Cardiosphere-Derived Cells (icCDCs) Improves Cardiac Function by Stimulating Myocyte Proliferation in Non-Infarcted Hibernating Myocardium with No Effect in Normal Myocardium
Authors: Gen Suzuki, Merced M Leiker, Thomas R Cimato, John M Canty
Journal: Circulation (2011)

Background: icCDCs have been shown to improve function and reduce fibrotic area when regionally infused into infarcted myocardium. Their ability to improve flow and function in viable dysfunctional myocardium in the absence of scar is unclear. Methods: Swine (n=12) with collateral-dependent hibernating myocardium from a chronic LAD occlusion were studied 4-months after instrumentation in the closed-chest propofol sedated state. CDCs were isolated from myocardial biopsies at the time of initial instrumentation. We assessed regional flow, LAD wall thickening (%LADWT) and global EF before and 4 weeks after icCDCs (∼40x10 6 cells) into each of the 3 major coronary arteries. Autologous (n=3) as well as non-autologous CDCs with cyclosporine treatment (200mg/day; n=3) had similar effects and results were pooled and compared to untreated swine (n=6). Histological analysis was performed to assess the frequency of Ki-67 and phospho-Histone H3 (pHH3) positive myocytes along with myocyte nuclear density and myocyte diameter. Results: Infusion of icCDCs in normal animals (icCDCs; n=5 vs. untreated; n=6) did not affect regional (%LADWT 51±1% vs. 48±3%, p-ns) or global function (EF: 70±2% vs. 70±3%, p-ns). In contrast, in hibernating myocardium, %LADWT increased from 23±6 to 51±5 % (p&lt;0.01) and EF increased from 57±1 to 71±4 % (p&lt;0.01) after icCDCs. There was no change in LAD coronary flow reserve (adenosine vasodilated flow/ resting flow 1.88 ± 0.20 to 2.03 ± 0.31, p-ns). icCDCs increased the number of Ki-67 and pHH3 positive myocytes (Table). This was accompanied by a prominent increase in myocyte nuclear density with reduced myocyte size suggesting the formation of new myocytes. Conclusion: We conclude that icCDCs result in a robust improvement in function in hibernating myocardium. This is not secondary to increases in myocardial perfusion but is secondary to pronounced myocyte proliferation and regeneration with newly formed myocytes in diseased but not normal myocardium.
Human exposure assessment and the National Toxicology Program.
Authors: G W Lucier, A Schecter
Journal: Environmental Health Perspectives (1998)

The National Institute of Environmental Health Sciences/National Toxicology Program (NIEHS/NTP) is developing a new interagency initiative in exposure assessment. This initiative involves the NIEHS, the Centers for Disease Control and Prevention through its National Center for Environmental Health, the National Institute for Occupational Safety and Health, the EPA, and other participating institutes and agencies of the NTP. This initiative will benefit public health and priority setting in a number of ways. First, as discussed above, it will strengthen the scientific foundation for risk assessments by the development of more credible exposure/response relationships in people by improving cross-species extrapolation, the development of biologically based dose-response models, and the identification of sensitive subpopulations and for "margin of exposure" based estimates of risk. Second, it will provide the kind of information necessary for deciding which chemicals should be studied with the limited resources available for toxicological testing. For example, there are 85,000 chemicals in commerce today, and the NTP can only provide toxicological evaluations on 10-20 per year. Third, we would use the information obtained from the exposure initiative to focus our research on mixtures that are actually present in people's bodies. Fourth, we would obtain information on the kinds and amount of chemicals in children and other potentially sensitive subpopulations. Determinations of whether additional safety factors need to be applied to children must rest, in part, upon comparative exposure analyses between children and adults. Fifth, this initiative, taken together with the environmental genome initiative, will provide the science base essential for meaningful studies on gene/environment interactions, particularly for strengthening the evaluation of epidemiology studies. Sixth, efficacy of public health policies aimed at reducing human exposure to chemical agents could be evaluated in a more meaningful way if body burden data were available over time, including remediation around Superfund sites and efforts to achieve environmental justice. The exposure assessment initiative is needed to address public health needs. It is feasible because of recent advances in analytical technology and molecular biology, and it is an example of how different agencies can work together to better fulfill their respective missions.
TCT-433 Impact of Beta-Blocker on Angiographic and Clinical Parameters during Intracoronary Acetylcholine Provocation Test
Authors: Sung Il Im, Byoung Geol Choi, Se Yeon Choi, Cheol Ung Choi, Seong Woo Han, Sun Won Kim, Jin Won Kim, Eung Ju Kim, Hong Euy Lim, Jin Oh Na, Dong Joo Oh, Chang Gyu Park, Seung-Woon Rha, Hong Seog Seo
Journal: Journal of the American College of Cardiology (2012)
Abstract 15681: Comparison of Five-year Outcomes of Percutaneous Coronary Intervention With Drug-eluting Stent Versus Bypass Surgery for Patients With Unprotected Left Main Coronary Artery Disease: Impact of Renal Insufficiency
Authors: Yasushi Fuku, Syun Watanabe, Kazushige Kadota, Seiji Habara, Hiroyuki Tanaka, Tsuyoshi Goto, Kazuaki Mitsudo, Takeshi Shimamoto, Genichi Sakaguchi, Tatsuhiko Komiya
Journal: Circulation (2011)

Objective: We sought to evaluate the impact of renal insufficiency on the five-year outcomes of percutaneous coronary intervention (PCI) with drug-eluting stent (DES) or coronary artery bypass graf...
Abstract 1349: The Economic Consequences of Non-Adherence to Lipid-Lowering Therapy: Results From the Anglo-Scandinavian Cardiac Outcomes Trial (ASCOT)
Authors: Peter Lindgren, Jennifer Eriksson, Martin Buxton, Thomas Kahan, Neil R Poulter, Bjorn Dahlof, Peter S Sever, Hans Wedel, Bengt Jonsson
Journal: Circulation (2009)
Abstract 16932: Melatonin Inhibits Large Conductance, Calcium-Activated K Channel Activity in Porcine Coronary Artery Smooth Muscle Cells by Activating MT2-Receptors
Authors: Praveen Shukla, Fanrong Yao, P Borowicz, Chengwen Sun, Stephen ORourke
Journal: Circulation (2011)

Introduction: Increasing evidence indicates that melatonin plays a role in cardiovascular homeostasis by regulating blood vessel diameter, thereby impacting arterial blood pressure and local blood ...