tbuckley/cabot-search huggingface dataset:
Myeloma Globulins of Plasma-Cell Neoplasms in Inbred Mice. I. Immunoelectrophoresis of Serum, With Rabbit Antibodies Prepared Against Microsome Fractions of the Neoplasms
Authors: Michael Potter, Edward L. Kuff
Journal: JNCI Journal of the National Cancer Institute (1961)

Journal Article Myeloma Globulins of Plasma-Cell Neoplasms in Inbred Mice. I. Immunoelectrophoresis of Serum, With Rabbit Antibodies Prepared Against Microsome Fractions of the Neoplasms Get access Michael Potter, Michael Potter Laboratories of Biology and Biochemistry, National Cancer Institute,3Bethesda, Maryland Search for other works by this author on: Oxford Academic PubMed Google Scholar Edward L. Kuff Edward L. Kuff Laboratories of Biology and Biochemistry, National Cancer Institute,3Bethesda, Maryland Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 26, Issue 5, May 1961, Pages 1109–1137, https://doi.org/10.1093/jnci/26.5.1109 Published: 01 May 1961 Article history Received: 08 November 1960 Revision received: 14 February 1961 Published: 01 May 1961
Abstract 15270: Circadian Variation in SCN5A Driven by the Molecular Clock
Authors: Elizabeth A Schroder, Karyn A Esser, Jonathan C Makielski, Brian P Delisle
Journal: Circulation (2011)

Circadian rhythms, endogenously generated rhythms with a 24 hour-period, are driven by an intrinsic molecular clock which is described as a transcription/translation feedback system. In mammals Bma...
Abstract 4207: Comparison of 1st-Pass Myocardial Perfusion Imaging by 64-slice CT and by MRI
Authors: Bina Ahmed, George Gentchos, Stephen P Bell, Mei Lee Frankish, Michael Jerosch-Herold, Timothy Christian
Journal: Circulation (2008)

The purpose of this study was to compare the signal intensity (SI) and image characteristics of 1st-pass (FP) CT imaging vs. MR FP in an animal model of resting myocardial blood flow (MBF). Pigs (n=5) were scanned at rest using FP MRI perfusion imaging. Gd-DTPA was injected IV at 5cc/sec at 0.04mmol/kg. FP MR was acquired at 3T with 3 axial slices 3mm thick per R-R interval during bolus injection using a saturation recovery gradient echo sequence. ECG gated FP CT was performed immediately thereafter on a 64-slice scanner in axial mode with 10 2.5mm thick slices during bolus injection (5cc/sec) of 40% dilution of iopamidol 370. MBF was measured at rest using fluorescent microspheres between studies. SI was measured for baseline and peak myocardial enhancement phases. Contrast enhancement (CE)= (peak-baseline) SI/baseline SI X 100. Rest MBF was 0.60±0.15 ml/min/g. Temporal resolution was similar by technique but more slices were imaged by CT per R-R interval (10 vs 3) and spatial resolution was superior for FP CT. Image characteristics are shown in the table . Baseline (top) and peak enhancement (bottom) images for FP CT and MRI are shown in the figure . Results were similar when undiluted contrast was used for each modality. Despite similar absolute change in SI, CE during FP CT is markedly reduced compared to FP MR due to a more than 20-fold higher baseline SI of the myocardium.
Ruxolitinib As Pretreatment Before Allogeneic Stem Cell Transplantation For Myelofibrosis
Authors: Nicolaus Kröger, Haefaa Alchalby, Markus Ditschkowski, Dominik Wolf, Gerald Wulf, Tatjana Zabelina, Christine Wolschke, Thomas Stübig, Francis Ayuk
Journal: Blood (2013)
A Murine Model for Human Cord Blood Transplantation: Near-Term Fetal and Neonatal Peripheral Blood Cells Can Achieve Long-Term Bone Marrow Engraftment in Sublethally Irradiated Adult Recipients
Authors: Andromachi Scaradavou, Luis Isola, Pablo Rubinstein, Yelena Galperin, Vesna Najfeld, David Berlin, Jon Gordon, Rona S. Weinberg
Journal: Blood (1997)
Abstract 9726: Cigarette Smoking: Association Between Inflammation, Subclinical Atherosclerosis and Cardiovascular Events. The Multi-Ethnic Study of Atherosclerosis (MESA)
Authors: John W Mcevoy, Michael J Blaha, Joao A Lima, David A Bluemke, Gregory Hundley, James K Min, Leslee J Shaw, Donald M Lloyd-Jones, Graham Barr, Matthew J Budoff, Roger S Blumenthal, Khurram Nasir
Journal: Circulation (2011)

Background: Cigarette smoking is known to increase cardiovascular event risk. Whether smoking continues to be associated with adverse events after taking into account the burden of subclinical atherosclerosis and vascular inflammation is unclear. Methods: MESA is a population-based study of 6815 adults free of baseline cardiovascular disease. We stratified participants based on smoking status into 3 groups: Never (NS), Former (FS), and Current Smokers (CS). FS and CS were further stratified into quartiles of pack-year history of tobacco exposure (PYH). We excluded 28 participants with missing information on smoking (n=23) or follow-up events (n=5). We analyzed the association of smoking status with CHD (coronary heart disease) and CVD (cardiovascular disease) events after taking into account traditional risk factors, CRP, as well as burden of CAC. Results: A total of 6787 participants were followed for median 5.8 years. CS comprised 887 (13%, mean age 58yrs), FS 2484 (37%, 63yrs) and NS 3416 (50%, 62yrs). Significantly more CS suffered both CHD and CVD events than NS (4.5% vs 3.3% [p=0.003] and 6.5% vs 4.9% [p=0.019], respectively). Multivariable HRs of 1.8 (CHD, p=0.004) and 1.9 (CVD, p&lt;0.001) were found in CS, compared to NS. These findings were increased with higher PYH, suggesting a dose-response effect of smoking on events. Incremental reductions in these HRs were found on addition of CRP (e.g. CVD HR=1.8, p&lt;0.001) and then CAC (e.g. CVD HR=1.5, p=0.012) to the multivariable adjusted regression model. No differences in event HRs were found between FS and NS. Conclusions: CS, but not FS, was strongly associated with events. This association weakened slightly with the inclusion of CRP, and more significantly with the inclusion of CAC. However, the persistent association of smoking with events even after accounting for the burden of inflammation and CAC confirms the importance of additional pathways contributing to the cardiovascular risk associated with smoking.
Abstract 3041: Calcification in Target Lesion and Stent-delivery Pathway as Important Determinant for Coronary Restenosis after Sirolimus-Eluting Stent Implantation: Calcification Score Analysis
Authors: Shumpei Mori, Yu Kataoka, Satoshi Yasuda, Isao Morii, Atsushi Kawamura, Shunichi Miyazaki
Journal: Circulation (2006)
Abstract 3354: Persistent Impairment of Endothelial Function is a Predictor of Adverse Outcomes in Patients with Coronary Artery Disease
Authors: Yoshinobu Kitta, Hajime Takano, Takamitsu Nakamura, Yasushi Kodama, Ken-ichi Kawabata, Jyun-ei Obata, Akira Mende, Masahiko Iwaoka, Makoto Abe, Kiyotaka Kugiyama
Journal: Circulation (2006)

Although endothelial dysfunction is a predictor of cardiovascular events, it remains unclear whether reversibility of endothelial dysfunction in response to risk-factor reduction provides prognosti...
Abstract 16234: Twinning and Multiple Births: A Risk for Neonates With Congenital Heart Disease?
Authors: Yaroslavna Nosikova, Glen S Van Arsdell, Lucy Roche, Edward J Hickey
Journal: Circulation (2011)

Background The impact of twinning and birth multiplicity for neonates with congenital heart disease is not known. Methods All 1,618 infants admitted to our institution within 30 days of life for a ...
Stem Cell Mobilization With Plerixafor + G-CSF In Comparison To Cyclophosphamide + G-CSF and Time-To-Progression After Autologous Stem Cell Transplantation For Multiple Myeloma
Authors: Alfred L. Garfall, Andrew Dougherty, Dan T. Vogl, Brendan M Weiss, Adam D Cohen, David L. Porter, Una O'Doherty, Patricia Mangan, Kathleen Cunningham, Don L. Siegel, Edward A. Stadtmauer
Journal: Blood (2013)
Abstract 3946: Morbidity After Reports of Serious Heart Failure in Type 2 Diabetes Patients With Underlying Cardiovascular Disease: Results From PROactive
Authors: Erland Erdmann, John A Dormandy, Stuart Kupfer, John Yates, Robert Spanheimer, Robert Wilcox
Journal: Circulation (2006)

Patients with type 2 diabetes (T2D) are at increased risk for cardiovascular (CV) events and the development of heart failure (HF), which may be exacerbated by diabetes treatment. PROactive, a CV o...
Abstract 10220: Long Term Outcome of Diabetic Patients Undergoing Bilateral Internal Thoracic Artery Grafting
Authors: Eli Hemo, Gideon Uretzky, Nachum Nesher, Dmitry Pevni, Goni Katz, Amir Kramer, Yosef Paz, Itzhak Shapira, Rephael Mohr
Journal: Circulation (2011)

Objectives:  Although BITA is associated with improved survival, the use of this technique in diabetics is controversial due to the increased risk of sternal infection. On the other hand, diabetic patients may benefit from BITA grafting due to their accelerated atherosclerosis and the increased propensity for saphenous vein graft failure. The purpose of this study is to evaluate pre-operative and operative factors affecting early and long-term outcome in order to better select diabetic patients for BITA grafting.   Methods:  Between 1996 and 2006, 57 insulin treated and 685 oral treated diabetic patients underwent isolated skeletonized BITA grafting. There were 562 males and 180 females. 313 were younger than 65 years of age, 304 were between 65 and 74 and 127 were 75 or older.   Results:  The actual early mortality was lower than the logistic “Euroscore” calculated mortality (3.8% vs. 6.4%, P=0.022). Increased mortality was observed in patients with Peripheral Vascular Disease (PVD) (8.1% vs. 2.9% without PVD. P=0.011). Early post-operative morbidity included sternal infection (3.8%), cerebro-vascular accident (1.7%) and peri-operative myocardial infarction (0.4%). Multivariate correlates of sternal infection were: repeat operation (P 75 years of age were 76.4%, 62.4%, and 38.6%, respectively (P   Conclusions:  BITA grafting in diabetic patients is safe. The favorable short and long-term outcome outweighs the adverse effect of sternal infection.
Abstract 2964: Outcomes in Patients with Adriamycin Cardiotoxicity Undergoing Orthotopic Heart Transplantation: An Analysis of the United Network for Organ Sharing Database
Authors: Cheri A Silverstein, Rebecca R Hung, Henry Ooi, Mark A Wigger, Douglas B Sawyer
Journal: Circulation (2008)

Background : Anthracyclines are the mainstay of breast cancer, leukemia, lymphoma and sarcoma treatment. Since approval of doxorubicin (adriamycin), the first and most widely used anthracycline, in 1974, cardiac toxicity has been the major complication of use of these drugs. Although orthotopic heart transplant (OHT) is considered an option for cancer survivors in complete remission, to our knowledge, the outcomes of OHT in these patients have not been reported. Methods : We reviewed the United Network for Organ Sharing (UNOS) database to identify transplants performed from 1988 through 2007. Individuals were stratified between a primary diagnosis of Dilated Myopathy: Adriamycin (DMA) and all other diagnoses. Survival rates at 1, 12, 36, 60 and 120 months post-transplant and proportions of deaths due to malignancy and rejection were reviewed. Results : From 1988 to 2007, 68,863 persons were listed with UNOS. Of those, 487 (0.71%) had a primary diagnosis of DMA. In the same period, 43,113 persons received OHT. Of those, 328 (0.76%) had a primary diagnosis of DMA. The DMA group had a younger mean age (37.6 vs. 45.9) and a higher fraction of females (199/328 (60.67% [55.38 – 65.96]) vs. 10447/42785 (24.42% [24.01–24.83]); (z = 15.2, p &lt; 0.001)) than all others. Survival at all time points was equivalent in DMA and all others (1mo: 94.12% [91.17–97.07] vs. 92.98% [92.74 –93.23]; 12mo: 86.53% [82.40 –90.66] vs. 84.79% [84.45–85.14]; 36mo: 78.31% [73.21– 83.42] vs. 77.37% [76.95–77.78]; 60mo: 69.73% [63.78 –75.69] vs. 70.53% [70.06 –71.00]; 120mo: 55.32% [48.03– 62.62] vs. 51.06% [50.44 –51.67]). Malignancy was reported as the primary cause of death in a similar portion of patients in both groups (10/115 (8.7% [3.55–13.85]) vs. 1901/17819 (10.67% [10.22–11.12]); (z = −0.682, p = 0.496)). Rejection was reported as a primary cause of death in a higher fraction of DMA patients (19/115 (16.52% [9.73–23.31]) vs. 1860/17819 (10.44% [9.99 –10.89]); (z = 2.12, p &lt; 0.05)). Conclusions : Individuals with cardiomyopathy due to anthracycline cardiotoxicity are not higher risk candidates for OHT. The cause of the higher fraction of reported deaths due to rejection in DMA patients is likely multifactorial, including the younger mean age and higher fraction of females in this group. This research has received full or partial funding support from the American Heart Association, AHA National Center.
Abstract 2132: Beta-Blocker Therapy and Effectiveness of Antitachycardia Pacing among ICD Patients: A Dose-Dependent Effect
Authors: Javier Jimenez-Candil, Jose Morinigo, Maria Ruiz Olgado, Claudio Ledesma, Cándido Martín-Luengo
Journal: Circulation (2008)

Beta-blockers (BB) increase the efficacy of antitachycardia pacing (ATP) in terminating slow and monomorphic ventricular tachycardias (MVT) in ICD patients. Whether this effect occurs in fast MVT and whether it is dose-dependent it is unknown. Our aim is to determine the relationship between the indexed dose equivalents (IDE) of beta-blockers (BB-IDE) with the effectiveness of ATP in terminating MVT. In this prospective study we included 200 ICD patients (LVEF: 31±11). Detection and ATP therapies for VT were programmed as follows: Fast-VT zone (Cycle Length [CL]: 250 –320 ms; 1 burst of 5 pulses at 84% of CL); slow-VT zone (CL: 321–390 ms; 3 bursts of 15 pulses at 91%). In each patient we determined the BB-IDE at MVT presentation. Dose equivalents (DE) were defined with atenolol used as reference. IDE were calculated by dividing DE by body surface area (mg*m2/day). During a follow-up of 602±368 days, 546 MVT (CL: 329±35 ms; 41% fast-VT; 22 % no BB treatment; median of BB-IDE: 26 mg*m2/day) were recorded. Success rate of APT was 87%. BB-IDE was higher in the cases of successful ATP (S-ATP): 22±18 vs. 15±16 (p=0.001). Classifying the events into three groups according to the BB-IDE: no BB, low IDE (BB-IDE&lt;median) and high IDE (BB-IDE≥median), the frequency of S-ATP increased with the BB-IDE: 78 vs. 84 vs. 94% (all MVTs; p&lt;0.001 for the trend) and 47 vs. 84 vs. 97% (fast-MVTs; p&lt;0.001, for the trend). In a multivariate analysis (logistic regression), BB-IDE (mg*m2/day) remained as a significant predictor of S-ATP: OR: 1.03 (95% CI: 1.01–1.05; p=0.001). The incidences of MVT-related symptoms and appropriate discharges were lower in higher values of BB-IDE. Table . Among ICD patients, BB therapy increases the likelihood of ATP terminating MVTs, especially in fast MVTs. This effect is dose-dependent: the higher the IDE, the more probable the S-ATP. Thus, BB improves MVT clinical tolerance. Frequencies of tachycardia-related symptoms and appropriate discharges with respect to BB-IDE
Abstract 1478: AMP-Activated Protein Kinase Phosphorylates Cardiac Troponin I and Alters Contractility in Murine Ventricular Myocytes
Authors: S Marisa Oliveira, Yin-Hua Zhang, Joanne Davies, David Carling, Barbara Casadei, Hugh Watkins, Charles Redwood
Journal: Circulation (2007)

In the heart, AMP-activated protein kinase (AMPK) is critical in the regulation of energy balance and in myocardial signaling, and is activated especially during ischaemia and exercise. It is a αβγ heterotrimer, with α being the catalytic subunit. Mutations affecting the regulatory γ2 subunit have been shown to cause a cardiac phenotype of hypertrophy and conduction disease, thus suggesting a specific role for this subunit in the heart. The γ isoforms are highly conserved at their C-termini but very different at their N-termini; hence we have investigated whether the unique N-terminus of γ2 could be involved in conferring substrate specificity or in determining intracellular localization. We have undertaken a GAL4 -based yeast two-hybrid assay to screen a human heart cDNA library using the N-terminal 273 residues of γ2 as bait. Five proteins were identified as true interactors in the yeast assay, one of these being cardiac troponin I (cTnI). In vitro studies showed that cTnI (isolated or reconstituted as troponin complex) is a good substrate for AMPK. Studies using site-specific cTnI mutants and mass spectrometry identified Ser-150 as the principal residue phosphorylated by AMPK. Actomyosin ATPase assays showed that phosphorylation at this residue resulted in increased Ca 2+ sensitivity of contractile regulation (ΔpCa 50 =+0.07). Treatment of cardiomyocytes with the AMPK activator AICAR for 15 min resulted in prolonged relaxation (time to 50% relaxation, TR50) in field stimulated left ventricular myocytes of C57B/6 mice (TR50 in ms: 29.8±1.4 in control and 38.0±2.4 with AICAR, n=11, 3 Hz and 35 o C). There was a tendency towards an increase in contraction with AICAR. Pretreatment of myocytes with AMPK inhibitor compound C (10 μM) abolished the effects of AICAR on myocytes (TR50 in ms: 30.9±1.7 before AICAR and 31.5±2.6 after AICAR, n=9). The effect of AICAR was mediated without altering the amplitude of the Ca 2+ transient, suggesting that it may be caused by a change in myofilament Ca 2+ -sensitivity and consistent with AMPK phosphorylation of cTnI. We hypothesize that cTnI phosphorylation by AMPK may represent a novel mechanism of regulation of cardiac function.
Abstract 9897: Impact of Extracorporeal Membrane Oxygenation and Cardiopulmonary Resuscitation on Norwood Procedure Outcomes: Data from the Single Ventricle Reconstruction Trail
Authors: Chitra Ravishankar, Sarah Tabbutt, Kerstin Allen, David S Cooper, Deborah U Frank, Peter C Frommelt, Nancy S Ghanayem, Caren S Goldberg, Eric M Graham, Joel A Kirsh, Catherine Dent Krawczeski, Wyman W Lai, Alan Lewis, Andrew J Lodge, Lynn Mahony, Janet Simsic, Lynn A Sleeper, Mario Stylianou, Yanli Wang, Peter Laussen
Journal: Circulation (2011)

Background: Extracorporeal membrane oxygenation (ECMO) and cardiopulmonary resuscitation (CPR) are reported risk factors for worse outcome after the Norwood procedure (NP). The Single Ventricle Reconstruction (SVR) trial randomized infants with hypoplastic left heart syndrome to a modified Blalock-Taussig shunt (MBTS) or right ventricular to pulmonary artery shunt (RVPAS). We explored associations between ECMO and/or CPR with death and transplant. Methods: Outcomes for 549 SVR patients using the shunt in place at end of the NP (MBTS 268, RVPAS 281) were compared among: CPR alone (CPR, n=37), ECMO alone (ECMO, n=49), ECMO required to regain circulation after CPR (E-CPR, n=36) and neither CPR nor ECMO (“none”, n=427). Cox proportional hazards regression with a time-dependent group indicator was used to model time to death or transplant. The interaction of patient group and shunt type was also examined. Results: Mean follow-up was 2.7 ± 1.9 years. Gestational age, age at surgery, size of ascending aorta, presence of significant tricuspid regurgitation, systolic function and shunt type were similar among the 4 groups. Low birth weight was more common in CPR or E-CPR groups (p&lt;.001). Patients receiving CPR, ECMO or E-CPR had a higher incidence of transplant or death (p&lt;.0001). With adjustment for surgeon and birth weight, interaction analysis showed poorer outcome with MBTS in the “none” group but not in the E-CPR group (interaction p = .014). The hazard ratio for MBTS vs. RVPAS in E-CPR was 0.48 (95% CI 0.21, 1.11) vs. 1.54 (95% CI 1.04, 2.28) for “none”. A similar pattern was seen with ECMO (interaction p = .047). A differential effect of shunt type was not observed for CPR vs. “none” patients. Conclusions: Patients requiring CPR and/or ECMO after the Norwood procedure have significantly increased early mortality and/or transplant and remain at risk for attrition remote from the initial event. The RVPAS was not found to be superior to the MBTS in patients requiring ECMO or E-CPR.
Expression profiles in pre-treatment biopsies from SCC of the head and neck correlate with outcome of radiotherapy.
Authors: Jan Akervall, Eugene Rontal, Michael Rontal, Jens Alm, Paulina Gyllerup, Ylva Gardinger, Johan Wennerberg, Sarah Scollon, Pete Haak, Haab Brian, Ed Dere, Kyle Furge, Bin Teh
Journal: Cancer Research (2006)

5184   Treatment decisions for patients with squamous cell carcinoma of the head and neck (SCCHN) rely heavily on TNM-classification. However, we have earlier shown that expression profiles can predict chemosensitivity. The aim of the present study was to evaluate potential genetic markers for prediction of response to radiotherapy in patients with SCCHN.  Material&Methods : Fresh frozen pre-treatment biopsies were obtained from 37 patients with SCCHN at the following sub-sites: oropharynx (n=22), hypopharynx (n=6) and other (oral cavity (n=7), larynx (n=2). They all received radiotherapy with intention to cure. cDNA micro array analysis was performed using slides containing 21,632 sequence validated human cDNAs, generally with insert sizes of 0.25-2.5 kb. For each experiment, mRNA of each cell line was analyzed using equally mixed mRNAs from the normal mucous membranes of nasopharynx, oropharynx, tongue, bucca, submaxillary gland, and base of tongue as a reference.  Results:  Supervised clustering revealed a genetic profile of 72 genes (e.g. caveolin 1, VEGF, Bcl-2 and INH being upregulated in radioresistent cases) that significantly distinguished patients that responded with Complete Response to radiotherapy from those with Partial response/No Response (p Conclusions : We identified mRNA expression patterns, as analyzed by cDNA micro array analysis, that distinguished radiotherapy responders from non-responders. These findings, along with previously reported chemosensitivity profiles could facilitate individualization of treatment of SCCHN based on the biological properties of the tumors, rather than TNM classification alone.
Abstract 1688: Mitochondrial Localization and Cellular Function of Cytoglobin: A Novel Calcineurin-Dependent Stress-Responsive Globin in the Cardiovascular System
Authors: Qiu Ye, Shilpa M Manda, Teresa D Gallardo, Sean C Goetsch, Daniel J Garry, Pradeep P Mammen
Journal: Circulation (2006)
P0774 : Ledipasvir/sofosbuvir with ribavirin is safe in &gt;600 decompensated and post liver transplantation patients with HCV infection: An integrated safety analysis of the solar 1 and solar 2 trials
Authors: D. Samuel, M. Manns, X. Forns, S.L. Flamm, K.R. Reddy, J. Denning, S. Arterburn, T. Brandt-Sarif, P.S. Pang, J.G. McHutchison, N. Afdhal, M. Charlton, E. Gane, D. Mutimer, G.T. Everson
Journal: Journal of Hepatology (2015)
The red cell mass-arterial oxygen relationship in normal man
Authors: John V. Weil, Gail Jamieson, Donald W. Brown, Robert F. Grover
Journal: Journal of Clinical Investigation (1968)

The normal relationship between red cell mass measured, with (51)chromium-labeled red cells, and arterial oxygen saturation (Sa(O2)) over the range from 97.3 to 83.4% was examined by studying 73 normal men residing at sea level and altitudes of 1600 and 3100 m. A simple, linear relationship between Sa(O2) and red cell mass was found over the entire range (r = - 0.7524, P < 0.001). In contrast, a correlation between red cell mass and arterial O(2) tension was found only over the lower half of the range of O(2) tensions where Sa(O2) was also decreased (r = - 0.7731, P < 0.005). This suggested that O(2) saturation rather than tension is the more important determinant of the erythropoietic response to chronic hypoxia. If this response is regulated by tissue O(2) tension, then it will be influenced by O(2) transport, which, in turn, is a function of blood flow and arterial O(2) content, and hence Sa(O2). In nine patients with chronic obstructive airway disease the relationship between red cell mass and Sa(O2) was also determined and was found to be steeper than in the normal subjects (P < 0.05).