tbuckley/cabot-search huggingface dataset:
Abstract 15972: Fibronectin is Essential for Mesendoderm Induction During Cardiac Differentiation of Human Pluripotent Stem Cells
Authors: Jianhua Zhang, Ran Tao, Douglas S Annis, Deane F Mosher, Timothy J Kamp
Journal: Circulation (2014)

Extracellular matrix (ECM) plays important roles in development, and we have previously demonstrated a matrix sandwich protocol for efficient cardiac differentiation of human pluripotent stem cells (hPSCs). The purpose of the present study was to identify the key ECM proteins required to initiate cardiogenic differentiation of hPSCs and define their mechanism of action. We tested purified ECM proteins including human laminin (LN111, LN521), fibronectin (FN) and collagen IV in the matrix sandwich protocol, and found FN promoted efficient cardiac differentiation. To determine if FN is essential for the early stages of cardiogenesis we tested three FN blockers: FUD peptide that binds the N-terminal 70-kDa fragment of FN, anti-human FN antibody clone10 which recognizes FN domain III2, and anti-human FN antibody clone 3E3 that binds on the cell attachment domain. All three blockers showed concentration-dependent inhibition of cardiac differentiation of hPSCs when added at day 0 for 24 hours, measured by flow cytometry for cTnT+ cells in the Matrigel/Matrigel (bottom/overlay) and FN/FN sandwich culture as well as in the small molecule GiWi protocol. Furthermore, clone10 showed significant inhibition of cardiac differentiation even in the absence of adding exogenous FN due to the robust endogenous expression of FN by the hPSCs. Gene expression analysis with qRT-PCR demonstrated that blocking FN by clone10 caused a significant suppression of gene expression related to: 1) epithelial to mesenchymal transition, e.g. SNAIL1, SNAIL2, GSC, VIM, CDH2 and FN1; 2) Mesendoderm e.g. T, MIXL1, SOX17 and precardiac mesoderm of MESP1; 3) Cardiac transcription factors ISL1 and NKX2.5. To investigate the cellular signaling activated by FN, we tested a blocking antibody (clone P5D2) to the major FN receptor, integrin β1, and found that P5D2 produced a concentration-dependent inhibition of cardiogenesis in multiple hPSC lines. Furthermore, the integrin-linked kinase (ILK) inhibitor, Cpd22, inhibited cardiac differentiation in a concentration-dependent fashion as well when added on day 0. We conclude that FN, acting via integrin β1-activated ILK signaling, is essential to mesendoderm formation enabling cardiogenesis of hPSCs.
Abstract 3789: Cardiac I-123 Metaiodobenzylguanidine Imaging Provides Additional Prognostic Power to Seattle Heart Failure Model in Patients With Chronic Heart Failure: A 10-year Follow-up Comparative Study
Authors: Yuki Kuramoto, Takahisa Yamada, Yuji Okuyama, Takashi Morita, Shoji Sanada, Yoshio Furukawa, Koji Tanaka, Yusuke Iwasaki, Taku Yasui, Hiromichi Ueda, Masato Kawasaki, Takeshi Okada, Shunsuke Tamaki, Wayne C Levy, Masatake Fukunami
Journal: Circulation (2009)

Background: The Seattle Heart Failure Model (SHFM) is a validated prediction model that estimates the mortality in chronic heart failure (CHF) by using commonly obtained clinical, laboratory, medic...
COR PULMONALE WITH BILATERAL ANEURYSMS OF THE PULMONARY ARTERY, INTERVENTRICULAR SEPTAL DEFECT, PATENT DUCTUS ARTERIOSUS AND TERMINAL AYERZA'S SYNDROME
Authors: M. W. Johannsen, Charles A. R. Connor
Journal: Annals of Internal Medicine (1943)

Case Reports1 February 1943COR PULMONALE WITH BILATERAL ANEURYSMS OF THE PULMONARY ARTERY, INTERVENTRICULAR SEPTAL DEFECT, PATENT DUCTUS ARTERIOSUS AND TERMINAL AYERZA'S SYNDROMEM. W. JOHANNSEN, M.D., CHARLES A. R. CONNOR, M.D.M. W. JOHANNSEN, M.D., CHARLES A. R. CONNOR, M.D.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-18-2-232 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAneurysms of the pulmonary artery are so rare as to constitute almost a curiosity. From 1905 to the present time there have been 28,180 autopsies in Bellevue Hospital and in only one case was this lesion found. The literature has been reviewed by D'Aunoy and E. von Haam1 and by Boyd and McGavack.2 We have found two cases out of 111 collected with lesions similar to the one here described. One was reported by Sachs3 and the other by Scott.4CASE REPORTThe patient (S. H.) in whom we found this lesion at necropsy was a 44-year-old Hungarian housewife. She...Bibliography1. D'AUNOYVON HAAM RE: Aneurysm of the pulmonary artery with patent ductus arteriosus, Jr. Path. and Bact., 1934, xxxviii, 39. CrossrefGoogle Scholar2. BOYDMCGAVACK LJTH: Aneurysm of the pulmonary artery, Am. Heart Jr., 1939, xviii, 562. CrossrefGoogle Scholar3. SACHS R: Weit offener ductus Botalli mit Bildung von (nichttuberculosen) Lungenarterienaneurysm, Deutsch. med. Wchnschr., 1892, xviii, 446. Google Scholar4. SCOTT RB: Aneurysm of the pulmonary artery, Lancet, 1934, i, 567. CrossrefGoogle Scholar5. BRENNER O: Sclerosis of the pulmonary artery with thrombosis, Lancet, 1931, i, 911. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New York, N. Y.*Received for publication November 26, 1940.From the Laboratories of Pathology and the Third (New York University) Medical Division, Bellevue Hospital. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byAneurysms of the Pulmonary ArteriesDuctus arteriosus persistensReferencesThe significance of the pulmonary vascular bed in congenital heart diseaseThe significance of the pulmonary vascular bed in congenital heart diseasePulmonary Artery AneurysmsAneurysm of the pulmonary artery: Review of the literature and report of a case 1 February 1943Volume 18, Issue 2Page: 232-237KeywordsAneurysmsAutopsyLesionsPatent ductus arteriosusPulmonary arteries ePublished: 1 December 2008 Issue Published: 1 February 1943 PDF downloadLoading ...
Fogarty at 40: NIH Center Updates Its Strategies for Supporting Global Health
Authors: Rebecca Voelker
Journal: JAMA (2008)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
THE LIMITATIONS OF GOVERNMENT IN MEDICINE; THE SAN FRANCISCO EXPERIENCE
Authors: J. C. Geiger, A. E. Larsen, J. P. Gray
Journal: Annals of Internal Medicine (1939)

Article1 November 1939THE LIMITATIONS OF GOVERNMENT IN MEDICINE; THE SAN FRANCISCO EXPERIENCEJ. C. GEIGER, M.D., F.A.C.P., A. E. LARSEN, M.D., J. P. GRAY, M.D.J. C. GEIGER, M.D., F.A.C.P.Search for more papers by this author, A. E. LARSEN, M.D.Search for more papers by this author, J. P. GRAY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-13-5-856 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptINTRODUCTIONRecent months, more than ever before, have seen the development of concentrated interest in and extensive discussion of the problems of medical care, particularly as to governmental participation in their solution. The mere fact that representatives of the various groups interested in the problem, from all sections of the country, have met in important conferences of national scope to discuss all aspects (medical, economic, sociologic and administrative) in itself demonstrates progress.American medicine today leads the world—scientifically and in all its humane aspects. Much of the discussion is built around public health and the lack of medical care. Public... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: San Francisco, California*Read at the New Orleans meeting of the American College of Physicians March 27, 1939. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 November 1939Volume 13, Issue 5Page: 856-871KeywordsPrevention, policy, and public health Issue Published: 1 November 1939 PDF downloadLoading ...
Guess the advert
Authors: C M Tyrie
Journal: BMJ (1999)

One day I found myself gazing at a picture of a man leaping over a bed in a room decorated with wallpaper patterned with boiled eggs. To my amazement I discovered I was looking at an advertisement for an inhaler ... and so started this quiz, which can be played anywhere, anytime, in the privacy of your own home, on the train, or on the plane. It's called “Guess the advert.” All you need is a handy copy of any medical publication which carries advertisements (ordinary commercial adverts are generally too transparent).

I undertook a straw poll among colleagues and friends on the subject of a number of recent adverts. The man leaping over the bed, mentioned above, was thought to be an advertistment for the National Lottery, the Egg Marketing Board, a bed manufacturer, and a breakfast cereal. Actually it was an advertisement for an inhaler. A woman with exotic dreadlocks was not representing Versace or Vivienne Westwood fashions—rather, a non-steroidal anti-inflammatory drug. A man leaping over a woman might, thought the sample, be advertising Viagra, or a stage show; in reality, it was advertising a statin.

Now try your hand at “guess the advert.” Possibilities—and the actual answers—are given at the bottom of the page. After this, pharmaceutical advertisements will never be quite the same!
Varicella vaccine in pregnancy
Authors: P. V Coyle, C. McCaughey, D. E Wyatt, H. J O'Neill, W. L Irving
Journal: BMJ (1997)

Data are not now collected by ethnic group in South AfricaEditor-Claire Hilton notes that since April 1995 British hospitals have had to collect data on each patient's ethnic group. 1 In the United States this is standard practice.In the Morbidity and Mortality Weekly Report data on numerous variables relating to the health and ill health of black and white people are regularly given 2 -as also, on occasion, are data on Hispanics, American Indians, Inuits, and Pacific Islanders.In South Africa precisely the converse has occurred.Ethnic subdivisions have stopped appearing in reports from the Central Statistics Services, and thus mortalities are no longer given for the constituent populations (black, Asian, coloured (Eur-African-Malay), and white), only for the combined population.Additionally, in our big cities annual reports from public health departments provide only combined data on morbidity and mortality.Disadvantages are many.Recently, colleagues and I reviewed changes in the total death rate and the death rate from coronary heart disease in South African populations. 3 We found that, from 1978 to 1989, mortality from coronary heart disease fell by 56% in the white population and by 36% in each of the Indian and coloured populations.Because changes in mortality from this foremost "killer" are continuing we intended to update our information.But this is now precluded.Currently, desegregation does not apply to our national cancer registry.But if it did how, for example, could benefits from Papanicolaou smear tests be assessed?The incidences of cervical cancer in the black and coloured populations are among the highest in the world.The constituent populations of South Africa have a wider divergence in the occurrence of a variety of diseases than occurs in any other country: coronary heart disease, appendicitis, colon cancer, and hip fractures.Interethnic differences in the occurrence of disease, reactivity, and metabolism are well known elsewhere. 4 In South Africa the change to using combined data will make it harder to identify and quantify the target populations who are in most need of help, as well as severely stultifying research on the occurrence of disease and on combating it.Hilton is right, of course, that the level of treatment is affected by ethnic group.Recently, in the United States the effect of race and income on mortality and the use of services among beneficiaries of Medicare was investigated; both race and income were shown as having substantial prejudicial effects, that of race being much more pronounced than that of income. 5This is a matter of universal shame.
Abstract 237: Favorable Prognosis in Cardiovascular Disease from In-Hospital Cardiac Arrest Developed Within 48 Hours of Hospital Admission: From the Japanese Registry of CPR for In-Hospital Cardiac Arrest (J-RCPR)
Authors: Taro Sasaoka, Naohiro Yonemoto, Hiroyuki Yokoyama, Hiroshi Nonogi
Journal: Circulation (2011)

Background: Recently, importance of in-hospital cardiopulmonary arrest (IHCPA) is recognized. Some studies showed the characteristics of IHCPA, however, the detail of IHCPA developed within 48-hours of hospital admission remains unknown. Method: A total of 491 adults with IHCPA were registered in J-RCPR from January 2008 to December 2009 as a multicenter prospective registry study. Among them, 182 IHCPA developed within 48-hours of hospital admission were retrospectively reviewed. They were divided in two groups according to the underlying disease, hospitalized for the treatment of cardiovascular disease (Group C, n=116, including ACS / Arrhythmia / Heart failure / Aortic diseases), and non- cardiovascular disease (Group N, n=66). Result: Baseline characteristics of sex, age, and pre-event consciousness were not statistically different in each group. The location of IHCA was significantly different, the prevalence of IHCPA in intensive care unit (ICU) and catheter laboratory were significantly higher in Group C (p&lt;0.01). The percentages of patients with ECG monitor and developed IHCPA within 10 min of the last checking time were significantly higher in Group C than Group N (90.4% vs. 75.5%, 87.1% vs. 74.2%, p&lt;0.05 respectively). In Group C (Group N), the prevalence of VF/VT as first documented rhythm was 37.8% (19.7%, p&lt;0.0001), asystole was 16.5% (36.3%, p&lt;0.0001), and pulseless electrical activity was 35.7% (44.0%, N.S.). Interestingly, in Group C, the rate of survival at 30 days after IHCPA was significantly higher than Group N (43.1% vs. 22.9%, p&lt;0.001), even though the rate of return of spontaneous circulation and of survival at 24-hours was not significantly different. The rate of the favorable neurological function (CPC 1-2) among patients survived at 30 days after IHCPA was also higher in Group C (86.0% vs. 63.6%, p&lt;0.05). Conclusion: Patients in Group C showed better survival than non-cardiovascular disease in this study; 37% of patients with cardiovascular disease developed IHCPA within 48-hours of hospital admission could recover without neurological deficiency. These results suggest the importance of careful observation of the patients in the first few days of hospital admission.
Abstract 16796: β2-Adrenergic Receptor Expression on Hematopoietic Cells is Critical for Survival Following Myocardial Infarction
Authors: Laurel A Grisanti, Ashley A Repas, Erhe Gao, Anna M Gumpert, Rhonda L Carter, Walter J Koch, Douglas G Tilley
Journal: Circulation (2014)

β-adrenergic receptors (βAR) are critical regulators of cardiac function normally and during (HF). The importance of βAR on cardiomyocyte contractility and survival is well defined however, following myocardial infarction (MI), inflammatory responses occur, which are critical for healing and scar formation. Catecholamines acting through βAR, particularly the β2AR subtype, are known to modulate immune responses, however, the influence of β2AR in regulating the inflammatory response following MI is unknown. To investigate the contribution of β2AR on immune cells following myocardial infarction (MI), wild-type (WT) mice were irradiated and then received β2ARKO or WT control BM transplants to create immune cell specific knockout (KO) animals. Following bone marrow reconstitution, mice were subjected to MI and cardiac function and survival were monitored. Cardiac function, as assessed by echocardiography, did not differ between WT and β2ARKO chimeric mice. However, mice lacking β2ARKO in their BM resulted in 100% mortality from cardiac rupture within two weeks of receiving MI in contrast to their WT counterparts that had ~20% death. Masson trichrome staining demonstrated infarct expansion in β2ARKO chimeric mice occurred more rapidly than their WT counterparts. Flow cytometric analysis showed decreased mobilization of granulocytes from bone marrow in β2ARKO mice. Additionally, β2ARKO chimeric mice reductions in infiltrating monocyte/macrophage, neutrophil and mast cell populations in the heart with no change in total cell infiltration suggesting a disruption in the ratio of infiltrating immune cells. Alterations in chemokine receptor levels, particularly CCR2, on BM resulted in decreased cellular migration. These results demonstrate the critical role of β2AR in mounting an immune response and promoting healing following MI.
Abstract 15416: Evidence for the Involvement of NADPH Oxidase in Adenosine Receptors-Mediated Control of Coronary Flow Using Knockout Mice
Authors: Mohammed El-Awady, Stephen L Tilley, S. Jamal Mustafa
Journal: Circulation (2011)

The NADPH oxidase (Nox) is the major source for reactive oxygen species (ROS) in cardiovascular system. In conditions such as ischemia-reperfusion injury and hypoxia, both ROS and adenosine are rel...
Abstract 804: Noninvasive Integrated SPECT/CT Molecular Imaging of Activated Factor XIII Activity in Thrombosis
Authors: Farouc A Jaffer, Jose L Figueiredo, Gregory Wojtkiewicz, Hanwen Zhang, Purvish Patel, Matthias Nahrendorf, Ching-Hsuan Tung, Ralph Weissleder
Journal: Circulation (2007)

Background : Activated factor XIII (FXIIIa) is a blood transglutaminase that mediates fibrinolytic resistance and is a hallmark of acute thrombi. Noninvasive molecular imaging of FXIIIa may offer a novel approach to identify acute thrombi and to gauge fibrinolytic resistance in vivo. Here we developed and validated a FXIIIa thrombosis imaging strategy using noninvasive integrated SPECT/CT. Methods: A FXIIIa-targeted peptide agent (F13) was synthesized using NQEQVSPLTLLK chelated to DOTA and then labeled with 111 InCl 3 . A control agent (C13, 111 In-NAEQVSPLTLLK) was analogously synthesized. In vitro validation of the F13 agent was performed in human plasma clots. Next, the in vivo blood-half life of F13 was determined in mice (n=4). In vivo thrombosis studies (n=15 mice) were then performed using 10% ferric chloride jugular venous thrombi aged 1 hour or 16 hours. Mice were intravenously injected with 200 μCi of F13 or C13. After 4 hours, mice underwent integrated CT angiography (72 μm isotropic resolution) and SPECT imaging (32 minute acquisition). In situ thrombi were then resected for radioactivity and weight measurements. Results : Human plasma clots incubated with F13 showed 280–740% greater counts per minute (CPM) than controls (p&lt;0.01). F13 binding was dose-dependent and &gt;90% inhibited by pretreatment with iodoacetamide, an alkylating agent. The blood half-life of F13 was calculated to be 16 minutes. In one hour thrombi, in vivo SPECT/CT imaging revealed strong focal F13 SPECT signal in the co-registered ipsilateral venous thrombi but not the contralateral normal jugular vein. One hour thrombi in the F13 group had 15-fold greater radioactivity than the C13 group (4.6±3.6% vs. 0.3±0.2% injected dose per gram tissue, IDGT, p&lt;0.01). Compared to 1 hour thrombi, 16 hour old thrombi had 4-fold less F13 radioactivity (1.1%±0.1% IDGT, p&lt;0.05). Conclusions : Blood transglutaminase FXIIIa can be noninvasively detected using a FXIIIa-sensitive and specific imaging agent for integrated SPECT/CT. The current in vivo results further validate that activated factor XIII is a hallmark of acute thrombi and declines in activity over time. This clinically translatable imaging strategy could permit visualization of FXIIIa in patients with thrombotic syndromes.
Atypical Features of Acute Coronary Occlusion
Authors: James B. Herrick
Journal: Annals of Internal Medicine (1929)

Article1 August 1929Atypical Features of Acute Coronary OcclusionJAMES B. HERRICKJAMES B. HERRICKSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-3-2-105 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptWhen I asked Dr. Means, our General Chairman, if the subject of acute coronary obstruction were not already old and well worn especially in Boston, he replied that he did not think the problem of coronary obstruction had been worked to death in the east. "The patients have died but not the subject which is ever present and very important." So, with this diplomatically worded official encouragement I venture once more to present this topic.Acute coronary occlusion was formerly regarded as a pathological curiosity. It is now looked upon as a readily recognizable clinical entity of rather frequent occurrence....1 The Weekly Roster and Medical Digest of The Philadelphia County Medical Society, May 23, 1925, p. 15. Google Scholar2 Gallavardin et Gravier: Formes Cliniques de l' Infarctus du Myocarde, Annales de Med., 1926, Sept., p. 161. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chicago, Illinois*Read at the Boston meeting of the College of Physicians, April 10, 1929. Nextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited by“Mild” myocardial infarctionMyocardial infarction indicated by angina pectoris of effort or by brief attacks of angina of rest, with remarks on premonitory painAn intimate account of my early experience with coronary thrombosis 1 August 1929Volume 3, Issue 2Page: 105-111 ePublished: 1 December 2008 Issue Published: 1 August 1929 PDF downloadLoading ...