tbuckley/cabot-search huggingface dataset:
Evaluation of assessment instruments for working alliance in psychological interventions with adolescents: A systematic review
Authors: Mariana Veloso Martins, Zorana Jolić Marjanović, Nuno Ferreira, Camellia Hancheva, Emma Motrico, Jose M Mestre, Nele A J De Witte, Sibel Halfon, Sidse Arnfred, Margarida Rangel Henriques, Nina Petričević, Marcin Rzeszutek, Jana Volkert, Randi Ulberg, Fredrik Falkenström
Journal: Clinical Psychology Review (2025)

The working alliance is one of the most robust predictors of outcomes in adult psychotherapy. Since the alliance is often challenging to establish and maintain in psychotherapy with adolescents, conducting high-quality assessments of the alliance using sound measures in this population is critical. Still, measurement instruments developed for adults cannot be directly transferred to adolescent samples. This systematic review aimed to identify and critically evaluate available assessment tools for working alliance in adolescent psychotherapy using the COnsensus-based Standards for the Selection of Health Measurement INstruments (COSMIN) framework. A comprehensive literature search across PsycINFO, PubMed, Web of Science, and PsycARTICLES, up to October 2024, yielded 47 research studies reporting on working alliance measurement properties. Findings indicate that self-report measures are most commonly studied, with the Working Alliance Inventory-Short Form (WAI-S) and Therapeutic Alliance Quality Scale (TAQS) showing the best psychometric properties. Nevertheless, even with these measures, there are notable shortcomings in cross-cultural validity, measurement error, and responsiveness, which are essential for applications in longitudinal studies and with diverse populations. Less commonly studied, often with very small samples, observer-rated tools displayed high reliability but limited predictive validity. Our review highlights the need for more stringent research on developmentally appropriate, reliable working alliance instruments for adolescents to support clinicians and researchers in studying and monitoring this aspect of patient-therapist relations. These findings, together with the COSMIN guidelines, inform recommendations for future research mainly in terms of improved content validity, measurement error, cross-cultural validity, and responsiveness.
Temporal Trends in the Childhood Opportunity Index at Children’s Hospitals
Authors: Samantha A. House, Matthew Hall, Clemens Noelke, Yang Lu, Victor Ritter, Bren Botzheim, Alicia Lew, Alan R. Schroeder
Journal: JAMA Pediatrics (2025)

This cohort study assessed annual changes in the Childhood Opportunity Index, a multidimensional measure of neighborhood conditions associated with health outcomes, for hospital-based encounters.
Trends over time and inter-hospital variation in the primary treatment approach for T1 colon carcinomas in the Netherlands
Authors: Julia Hanevelt, Felice N. van Erning, Wouter H de Vos tot Nederveen Cappel, Frank P. Vleggaar, H L van Westreenen, Leon MG Moons
Journal: Endoscopy (2025)

BACKGROUND AND STUDY AIMS This study evaluates the use of local resections (LR) as initial treatment versus primary surgery (PS) for T1 colon carcinoma (CC) in the Netherlands over time, hospital variations, and whether changes in treatment approaches impact 5-year relative survival (RS) and overall survival (OS). PATIENTS AND METHODS This nationwide cohort study included all patients diagnosed with pT1 adenocarcinoma of the colon between 2015-2022, identified from the Netherlands Cancer Registry (NCR). Multilevel, multivariable logistic regression models estimated the probability of undergoing LR per hospital, adjusted for case-mix variables. Hospitals were categorized into low, average, or high attitude towards LR. RS and OS were calculated using multivariable regression analysis. RESULTS A total of 9,650 patients from 73 hospitals were included, with 3,999 (41.4%) receiving PS and 5,651 (58.6%) undergoing LR first. From 2015 to 2022, the national proportion of PS decreased from 53.2% to 29.7%. The RRadj for LR varied across hospitals (RRadj 0.46-1.29). No significant differences in RS or OS were found between high- vs. low attitude centers (5-year RS 99.0% vs. 97.7%, RER 0.97, 95%CI 0.51-1.84 and OS 87.9% vs. 86.4%, HRadj 0.95, 95%CI 0.81-1.11), nor between patients treated after vs. before 2018 (5-year RS 98.7% vs. 98.7%, RER 0.82, 95%CI 0.46-1.46 and OS 86.7% vs. 88.0%, HRadj 0.98, 95%CI 0.85-1.13). CONCLUSIONS While inter-hospital variation exists, LR of T1 CC is increasingly preferred in the Netherlands, leading to a reduction in the number of surgeries without a change in RS or OS.
Remote pulmonary artery pressure‐guided management of patients with heart failure: A clinical consensus statement of the Heart Failure Association (<scp>HFA</scp>) of the <scp>ESC</scp>
Authors: Antoni Bayes‐Genis, Matteo Pagnesi, Pau Codina, William T. Abraham, Offer Amir, Rudolf A. de Boer, Jasper J. Brugts, Ovidiu Chioncel, Finn Gustafsson, JoAnn Lindenfeld, Wilfried Mullens, Mark C. Petrie, Giuseppe Rosano, Marco Metra
Journal: European Journal of Heart Failure (2025)

Abstract Episodes of worsening heart failure (HF) are a major cause of unplanned hospitalizations. Their onset is usually preceded by an early increase in intracardiac pressures with subsequent worsening of symptoms due to congestion. Implantable devices allowing daily remote pulmonary artery pressure (PAP) monitoring are useful to identify early haemodynamic changes so that medical therapy can be adjusted at an early stage, before symptom onset, and HF‐related hospitalizations be prevented. Second, the use of these devices may help to maintain clinical stability keeping PAP in the target range on a day‐to‐day basis. The CardioMEMS system allows remote PAP monitoring, and PAP‐guided medical therapy has reduced HF‐related hospitalizations in prospective, randomized, controlled clinical trials in symptomatic patients with HF, independent of their left ventricular ejection fraction. The safety and feasibility of other devices, like the Cordella implantable PAP sensor, have also been demonstrated and clinical usefulness in larger patient populations is currently being assessed in several trials. Most of the studies testing remote PAP monitoring were reported after the 2021 European Society of Cardiology HF guidelines. An update of the clinical significance and potential implications for clinical practice of these systems seems therefore warranted. The aim of this clinical consensus statement is to summarize current knowledge on remote PAP‐guided management of patients with HF, with a special focus on current evidence from clinical trials, potential impact on clinical practice and management aspects.
Strategies to overcome tumour relapse caused by antigen escape after CAR T therapy
Authors: Yufei Lu, Fu Zhao
Journal: Molecular Cancer (2025)

Chimeric antigen receptor (CAR) T cell therapy has revolutionized the treatment of B cell and plasma cell malignancies, and numerous promising targets against solid tumours are being explored. Despite their initial therapeutic success in hematological cancers, relapse occurs in a significant fraction of patients, highlighting the need for further innovations in advancing CAR T cell therapy. Tumour antigen heterogeneity and acquired tumour resistance leading to antigen escape (antigen loss/downregulation) have emerged as a crucial factor contributing to immune escape and CAR T cell resistance, particularly in the case of solid tumours with only limited success achieved to date. In this review, we discuss mechanisms of tumour relapse in CAR T cell therapy and the promising strategies that are under development to overcome multiple resistance mechanisms, thereby reducing outgrowth of antigen escape variants. Specifically, we emphasize the importance of designing clinical translational strategies to enhance CAR T cell crosstalk with host immune cells, eliciting endogenous antitumour immune responses through antigen/epitope spreading, which offers a genuine solution to the limitations of targeting tumour antigen heterogeneity in solid tumours with monospecific T cell therapies.
Epigenomic disorder and partial EMT impair luminal progenitor integrity in Brca1-associated breast tumorigenesis
Authors: Camille Landragin, Melissa Saichi, Marthe Laisné, Adeline Durand, Pacôme Prompsy, Renaud Leclere, Jérémy Mesple, Kyra Borgman, Amandine Trouchet, Marisa M Faraldo, Aurélie Chiche, Anne Vincent-Salomon, Hélène Salmon, Céline Vallot
Journal: Molecular Cancer (2025)

In breast cancer related to the BRCA1 mutation, luminal progenitor cells are believed to be the cells of origin, yet how these cells transform into invasive cancer cells remain poorly understood. Here, we combine single-cell epigenomic and transcriptomic data to reconstitute sequences of events in luminal cells that lead to tumorigenesis. Upon deletion of Trp53 and Brca1, we find that luminal progenitors display an extensive epigenomic disorder associated with a loss of cell identity. These cells then progress to tumor formation through a partial epithelial-to-mesenchymal transition, orchestrated by Snail and the timely activation of immunosuppressive and FGF signaling with their microenvironment. In human samples, pre-tumoral changes can be detected in early stage, basal-like tumors, which rarely recur, as well as in normal-like mammary glands of BRCA1 mutation carriers who have had cancer. Our study fills critical gaps in our understanding of BRCA1-driven tumorigenesis, opening perspectives for the early monitoring of individuals with high cancer risk.
Comparative Analysis of Circulating and Synovial Immune Cells in Early Untreated Rheumatoid Arthritis and their relationship with molecular pathology and disease outcomes
Authors: Felice Rivellese, Elena Pontarini, Liliane Fossati‐Jimack, Rita A. Moura, Vasco C. Romão, João Eurico Fonseca, Alessandra Nerviani, Cankut Çubuk, Katriona Goldmann, Giovanni Giorli, Myles Lewis, Michele Bombardieri, Costantino Pitzalis
Journal: Arthritis & Rheumatology (2025)

Objectives To assess the relationship of circulating and synovial immune cells with synovial molecular pathology and disease outcomes in early Rheumatoid Arthritis (RA). Methods Early (&lt;12 months) treatment‐naïve RA patients (n=144) from the Pathobiology of Early Arthritis Cohort (PEAC) were included in this posthoc analysis. Following ultrasound‐guided synovial biopsy of the most active joint, patients received standard‐of‐care treatment and were followed up for 12 months. Synovial biopsies were analysed by immunohistochemistry and classified into synovial pathotypes. Peripheral blood samples (n=70) underwent flow cytometry, while RNA‐sequencing from synovial tissue (n=144) and matched peripheral blood (n=55) were analysed using signature‐based deconvolution. Results T‐peripheral‐helper cells (Tph), identified by flow cytometry, were the only circulating immune cell subset positively correlated with both systemic (ESR and CRP) and local inflammation (ultrasound synovial thickening and synovitis score). Circulating Tph cells were also significantly higher in seropositive patients and patients with lympho‐myeloid synovitis. Conversely, circulating B‐cells showed a significant inverse correlation with inflammatory markers, ultrasound scores, and disease activity. Signature‐based deconvolution of matched synovial and peripheral blood identified divergent immune cell signatures. While peripheral blood signatures showed no associations with longitudinal outcomes, synovium signatures were linked to clinical outcomes. In particular, patients achieving remission at 6 months (DAS28&lt;2.6) had higher baseline synovial Tph signatures and a greater post‐treatment reduction of the Tph signature. Conclusions Early untreated RA patients showed divergent immune cell signatures between synovia and peripheral blood. Tph cells, in both compartments, emerged as key markers for inflammation, disease activity, and treatment response.
Expert Perspectives on a Clinical Challenge: Hematologic Malignancies and Vasculitis
Authors: Michelle L. Robinette, Hetty E. Carraway
Journal: Arthritis & Rheumatology (2025)

Systemic vasculitis describes a collection of rare diseases each caused by inflammation of blood vessel walls that can cause severe systemic complications [[3, 4]](#ref‐0003). The pattern of vascular inflammation and resultant damage is frequently variable and may be mimicked, or directly triggered, by infection or malignancy. Myeloid and lymphoid hematologic malignancy have long been recognized in association with vasculitis and were recognized by the 2012 Consensus Conference on the Nomenclature of Systemic Vasculitis as vasculitis associated with probable etiology (cancer‐associated) [[4]](#ref‐0004). We will refer to this as vasculitis with concomitant hematological malignancy (VCHM). This is a rapidly evolving field with limited but growing evidence base. Due to increased use of molecular diagnostics in hematologic clinical practice, rheumatologists should be aware of the diagnostic and treatment complexity in rare patients with VCHM, who often have refractory disease and are at risk of comorbidity from both hematologic and inflammatory disease and their treatments. Here, we discuss our collaborative approach to diagnosis and management of VCHM shared between rheumatology and hematology/oncology, in the context of relatively limited literature. We focus on myeloid neoplasia, as a clinically relevant model of the emerging recognition of overlap between our fields, with more limited discussion of associations with lymphoid forms of VCHM such as cryoglobulinemic vasculitis that have been previously discussed in the literature
Racial and ethnic differences in HPV-related cancer incidence in the United States, 2001-2020
Authors: Adino Tesfahun Tsegaye, Jaimie Z Shing, Jacqueline B Vo, Aimée R Kreimer, Meredith S Shiels
Journal: JNCI Journal of the National Cancer Institute (2025)

Abstract Background Human papillomavirus (HPV) causes cervical cancer and a proportion of oropharyngeal, vulvar, vaginal, penile, and anal cancers. Evaluating racial and ethnic heterogeneity by anatomic site will identify populations with the highest cancer incidence rates (IRs) and help to optimize available prevention strategies. Methods Using the 2001-2020 US Cancer Statistics database, we estimated age-standardized IRs cervical carcinoma, oropharyngeal, anal, vaginal, vulvar, and penile squamous cell carcinomas (SCCs) by race and ethnicity. We examined changes over time by comparing IRs in 2016-2020 with 2001-2005. Results Between 2001-2020, 750,897 HPV-related cancers occurred among 6,168,715,419 total person-years, with 61% (n = 455,475) in females. Among females, the highest IRs of oropharyngeal (1.6/100,000 person-years), vulvar (2.3/100,000 person-years), and anal (2.1/100,000 person-years) SCC were among White females. The highest IR for vaginal SCC (0.6/100,000 person-years) was among Black females and for cervical carcinoma (10.0/100,000 person-years) among Hispanic females. Among males, the highest IR for oropharyngeal SCC (8.0/100,000 person-years) was among White males, penile SCC (1.3/100,000 person-years) among Hispanic males, and anal SCC (1.5/100,000 person-years) among Black males. From 2001-2005 to 2016-2020, for most racial and ethnic groups, both in terms of absolute incidence, and proportion of the total HPV-related cancer burden, cervical carcinoma and vaginal SCC rates decreased, vulvar and anal SCC increased, and there was no clear pattern in oropharyngeal and penile SCC rates. Conclusion For all cancer types, there were disparate racial and ethnic patterns by anatomic site likely caused by a constellation of factors, including access to preventive care and site-specific HPV prevalence.
Gut microbes–spinal connection is required for itch sensation
Authors: Tong Jin, Si-Yuan Li, Hong-Li Zheng, Xiao-Dan Liu, Yue Huang, Gan Ma, Ya-Xuan Zhao, Xiao-Tian Zhao, Li Yang, Qi-Hui Wang, Hong-Jun Wang, Chengyong Gu, Zhiqiang Pan, Fuqing Lin
Journal: Gut Microbes (2025)

The gut microbiota has been linked to a number of neurological disorders. However, it is unclear whether the gut microbiota is involved in the genesis of chronic itch, a refractory condition that afflicts patients both physically and mentally. Here, we report that depletion of gut microbiota enhances tolerance to itch in mice orally administered with antibiotics (ABX) and mice free of germ. Of note, oral gavage with Bacteroides fragilis (B. fragilis), a prominent species of the genus Bacteroides with most differential change, corrected the ABX-induced itch dysfunction through its driven metabolite acetyl-l-carnitine (ALC). Mechanistically, gut microbiota or B. fragilis depletion caused a decrease in RNA N6-methyladenosine (m6A) demethylase FTO expression in the dorsal horn and a consequent increase in RNA m6A sites in Mas-related G protein-coupled receptor F (MrgprF) mRNA, leading to decreased MRGPRF protein. The downregulation of FTO was triggered by inactivation of ETS proto-oncogene 1 (ETS1), a transcription factor that binds to the Fto promoter. These findings support a gut microbe - spinal connection in modulation of itch sensation in RNA m6A epigenetic-dependent manner and highlight a critical role of ALC in linking the altered B. fragilis and itch dysfunction.
High-resolution analysis of the treated coeliac disease microbiome reveals strain-level variation
Authors: Jelle Slager, Hanna L. Simpson, Ranko Gacesa, Lianmin Chen, Ineke L. Tan, Jody Gelderloos, Astrid Maatman, Cisca Wijmenga, Alexandra Zhernakova, Jingyuan Fu, Rinse K. Weersma, Gieneke Gonera, Iris H. Jonkers, Sebo Withoff
Journal: Gut Microbes (2025)

Coeliac disease (CeD) is an immune-mediated disorder primarily affecting the small intestine, characterized by an inflammatory immune reaction to dietary gluten. CeD onset results from a multifaceted interplay of genetic and environmental factors. While recent data show that alterations in gut microbiome composition could play an important role, many current studies are constrained by small sample sizes and limited resolution. To address these limitations, we analyzed fecal gut microbiota from two Dutch cohorts, CeDNN (128 treated CeD patients (tCeD), 106 controls) and the Lifelines Dutch Microbiome Project (24 self-reported tCeD, 654 controls), using shotgun metagenomic sequencing. Self-reported IBS (570 cases, 1710 controls) and IBD (93 cases, 465 controls) were used as comparative conditions of the gastrointestinal tract. Interindividual variation within the case and control groups was calculated at whole microbiome and strain level. Finally, species-specific gene repertoires were analyzed in tCeD patients and controls. Within-individual microbiome diversity was decreased in patients with self-reported IBS and IBD but not in tCeD patients. Each condition displayed a unique microbial pattern and, in addition to confirming previously reported microbiome associations, we identify an increase in the levels of Clostridium sp. CAG:253, Roseburia hominis, and Eggerthella lenta, amongst others. We further show that the observed changes can partially be explained by gluten-free diet adherence. We also observe increased interindividual variation of gut microbiome composition among tCeD patients and a higher bacterial mutation frequency in tCeD that contributes to higher interindividual variation at strain level. In addition, the immotile European subspecies of Eubacterium rectale, which has a distinct carbohydrate metabolism potential, was nearly absent in tCeD patients. Our study sheds light on the complex interplay between the gut microbiome and CeD, revealing increased interindividual variation and strain-level variation in tCeD patients. These findings expand our understanding of the microbiome's role in intestinal health and disease.
A Self‐Assembled Nanoreactor for Realizing Antibacterial Photodynamic/Gas Therapy and Promoting Wound Healing
Authors: Chenyang Jia, Ling‐Yi Li, Qiu‐Yi Duan, Xiaoyang Liu, Xiao‐Yu Zhu, Ke‐Fei Xu, Chengcheng Li, Zi‐Xi Wang, Fu‐Gen Wu
Journal: Advanced Healthcare Materials (2025)

Abstract Among various treatments employed to solve the global problem of bacterial infection, photodynamic therapy (PDT) is recognized as a method with great potential to inactivate a wide range of bacteria without the development of drug resistance. However, many commonly used photosensitizers (PSs) have the disadvantages of poor water‐solubility and potential toxicity, which limits their clinical application. Additionally, nitric oxide (NO) has unique advantages in antibacterial treatments due to its small molecular weight. Herein, protoporphyrin IX (PpIX), L‐arginine (L‐Arg), and glycol chitosan (GC) are used to construct a self‐assembled cationic Arg‐GC‐PpIX nanoreactor for efficient bacterial inactivation under white light illumination. The Arg‐GC‐PpIX nanoreactor with excellent water dispersity and stability can rapidly bind to bacteria through electrostatic interaction and produce local singlet oxygen ( 1 O 2 )/NO under light irradiation, leading to a high antibacterial efficiency toward both Gram‐negative and Gram‐positive bacteria. Besides, these NPs also possess a desirable antibiofilm ability. Finally, Arg‐GC‐PpIX@Gel which is obtained through loading Arg‐GC‐PpIX into the sodium alginate (SA)/Ca 2+ hydrogel shows a satisfactory ability to promote infected wound healing when combined with white light irradiation. Therefore, the rationally designed Arg‐GC‐PpIX nanoreactor with light‐triggered 1 O 2 /NO release is a promising antibacterial agent for achieving effective PDT/NO gas therapy.
Controlled Magnesium Ion Delivery via Mg‐Sputtered Nerve Conduit for Enhancing Peripheral Nerve Regeneration
Authors: Hyewon Kim, Jieun Kwon, Hyeok Kim, Sunhee Lee, Seongchan Kim, Ji‐Young Lee, Khandoker Asiqur Rahaman, Taeyeon Kim, Hyojin Lee, Myoung‐Ryul Ok, Seok Chung, Hyung‐Seop Han, Yu‐Chan Kim
Journal: Advanced Healthcare Materials (2025)

Abstract Autologous nerve grafting remains the gold standard for treating peripheral nerve injuries; however, it is constrained by limited donor nerve availability, the need for secondary surgeries, and sensory loss at the donor site. Biodegradable material‐based nerve conduits have emerged as a promising alternative to address these limitations and enhance nerve regeneration. Among these materials, magnesium stands out due to its exceptional biocompatibility, biofunctionality, and neuroprotective properties. Despite its potential, magnesium's rapid corrosion rate and the need for controlled ion release necessitate advanced modifications, such as the development of Mg alloys. However, these approaches often face challenges, including viability concerns and material hardness, which can hinder nerve repair and damage surrounding tissues. In this study, a novel solution is introduced by sputtering magnesium onto a soft collagen sheet, achieving controlled magnesium ion release while preserving the material's nerve‐like softness. This Mg‐sputtered collagen sheet demonstrates excellent biocompatibility and significantly improves axon regeneration, muscle reinnervation, and functional recovery in a sciatic nerve defect model. These findings highlight the potential of an innovative Mg‐based biodegradable nerve conduit, offering transformative applications across various medical fields.