tbuckley/cabot-search huggingface dataset:
TCT-943 Durable Valve Prosthesis Performance and Sustained Symptom Relief: 2-Year Outcomes Following Transseptal Transcatheter Mitral Valve Replacement in the HighLife TSMVR Feasibility Study
Authors: Mirjam Keßler, Christophe Dubois, Didier Tchetche, Wojtek Wojakowski, Zenon Huczek, Georg Nickenig, Wolfgang Rottbauer
Journal: Journal of the American College of Cardiology (2024)
TCT-964 Gender Disparities and Cardiovascular and Transplant-Associated Outcomes of Heart Transplant: National Readmission Database Transplant Cohort
Authors: Sanchit Duhan, Manoj Kumar, Syed Ishaq, Bijeta Keisham, Saad Ur Rahman, Dipan Uppal, Waleed Alruwaili, Shafaqat Ali, Mustafa Sajjad Cheema, Kunal Patel, Yasar Sattar
Journal: Journal of the American College of Cardiology (2024)
TCT-937 Utilization of Upfront Mechanical Thrombectomy Is Associated With Better Immediate Outcomes in Patients With ST Elevation Myocardial Infarction
Authors: Zafer Sattouf, Danial Nasif, Nour Tashtish, Carl Gillombardo, Abdallah Dweekat, Matthew Nied, Mehdi Shishehbor, Anjan Gupta
Journal: Journal of the American College of Cardiology (2024)
TCT-928 Comparison of the Self-Expandable Acurate Neo 2 and the New Balloon-Expandable Myval Series Transcatheter Heart Valve at 4-Year Follow-Up
Authors: Akash Jain, John Jose, Matteo Montorfano, Henrik Nissen, Pedro Martin, Ashok Seth, Mario Garcia-Gomez, Clara Fernandez Cordon, Alberto Campo Prieto, Ignacio Amat-Santos
Journal: Journal of the American College of Cardiology (2024)
TCT-933 Win Ratio Analysis of the LANDMARK Trial: Myval Transcatheter Heart Valve vs Contemporary Standard Valves (Sapien and Evolut) in Patients With Severe Aortic Stenosis
Authors: Akihiro Tobe, Niels van Royen, Ignacio Jesús Amat Santos, Martin Hudec, Matjaz Bunc, Alexander Ijsselmuiden, Liesbeth Rosseel, Amr Gamal, Angela McInerney, Scot Garg, Osama Soliman, Yoshinobu Onuma, Andreas Baumbach, Patrick Serruys
Journal: Journal of the American College of Cardiology (2024)
TCT-931 Outcomes of Self-Expanding vs Balloon-Expandable Transcatheter Aortic Valves in Patients With Small Aortic Annuli: A Meta-analysis
Authors: Sheref Elseidy, Mounica Vorla, Mohamed Hamed, Manahil Rehan, John Isaac, Tamam Mohamad, Teresa Castiello, Mamas Mamas
Journal: Journal of the American College of Cardiology (2024)
TCT-974 Characteristics and Outcomes of Patients Screened out of Tricuspid Transcatheter Edge-to-Edge Repair or Transcatheter Tricuspid Valve Replacement Clinical Trials
Authors: Tai Pham, Andrea Soares, Edris Aman, Kwame Atsina, Jason Rogers, Gagan Singh
Journal: Journal of the American College of Cardiology (2024)
TCT-948 The Frequency of Subclinical Leaflet Thrombosis and Correlation With Implant Shape in Large Animal Model Studies
Authors: Mateusz Kachel, Karolina Lukasik, Maksymilian Grajek, Krzysztof Sanetra, Marta Mazur, Wojciech Fil, Adam Janas, Carlos Fernandez, Paweł Kaźmierczak, Piotr Buszman
Journal: Journal of the American College of Cardiology (2024)
TCT-961 Immune Thrombocytopenic Purpura Is an Independent Risk Factor for Prosthetic Aortic Valve Degeneration: Analysis of the National Inpatient Sample Database 2019
Authors: Palak Patel, Abbas Alshami, Anmol Johal, Steven Imburgio, Steven Douedi, Hira Akhlaq, Jesus Almendral, Matthew Schoenfeld, Renato Apolito
Journal: Journal of the American College of Cardiology (2024)
TCT-934 Early Administration of Heparin at First Medical Contact Versus in the Cath Lab for STEMI Patients Undergoing Primary Percutaneous Coronary Intervention (HELP-PCI): A Multicenter, Randomized Trial
Authors: Jing Chen, Changwu Xu, Liqiang Qiu, Bin Li, Wei Yao, Hui Wu, Zheng Hu, Shuo Yang, Da Luo, Jianfang Liu, Menglong Wang, Jian Yang, Hong Jiang
Journal: Journal of the American College of Cardiology (2024)
TCT-972 Short-Term Outcomes of Transcatheter Tricuspid Valve Interventions on Post-Procedural Length of Hospital Stay, Readmission for Heart Failure, and Procedure Success If an Intracardiac Device Is Present: A Systematic Review and Meta-Analysis in a New Era of Tricuspid Interventions
Authors: Dilesha Kumanayaka, Laura Hernandez Perez, Wajeeha Aiman, Yazeed Abu Ruman, Aishwarya Chandra, Palak Patel, Aharnish Patel, Addi Suleiman
Journal: Journal of the American College of Cardiology (2024)
TCT-938 Identification and Characterization of Microparticles and Microorganisms in Blood Clots of STEMI Patients: A Comparative Study of Rescue and Primary Angioplasty
Authors: Kelly Silva, Luiz Fernando Zanin, Patrícia Cristina Milanez, Jaqueline Pereira, Aline Santana, Francisco Fonsceca, Tiffany Menezes, Rita Sinigaglia-Coimbra, Maria de Lourdes Higuchi, Francisco Sandro Menezes-Rodrigues, Adriano Caixeta
Journal: Journal of the American College of Cardiology (2024)
TCT-936 Thrombectomy During Percutaneous Coronary Intervention in Patients With ST-Elevation Myocardial Infarction
Authors: Samarthkumar Thakkar, Ambica Nair, Parth Desai, Shilpkumar Arora, Syed Zaid, Joe Aoun, Sachin Goel, Neal Kleiman, Alpesh Shah
Journal: Journal of the American College of Cardiology (2024)
TCT-960 The Impact of Prior Coronary Artery Bypass Graft Surgery on Clinical Outcomes After Transcatheter Aortic Valve Replacement: A Propensity-Matched Comparison
Authors: Luis Gruberg, Shahan Haseeb, Wally Omar, Puneet Gandotra, Robert Kalimi, Andrew Persits, Bruce Rutkin, Chad Kliger, Jacob Scheinerman
Journal: Journal of the American College of Cardiology (2024)
TCT-968 Automatic Echocardiography Prompts Reveal Lack of Equitable Access to Care in Patients With Tricuspid Regurgitation: The Equitable Access Study III
Authors: Sophie Offen, Sarah Johns, Terra Boone, Georgia Kersche, Brady Robinson, Jia Wang, Katherine Kulyk, Julius Jelisejevas, Ali Husain, Kevin Millar, Hassan Ogran, Teresa Tsang, Christina Luong, Darwin Yeung, Michael Tsang, Erin Tang, Stephanie Sellers, Robert Moss, Christopher Thompson, Gnalini Sathananthan, Anson Cheung, Jian Ye, Arthur Lee, Nicholas Schnee, Richard Cook, Janarthanan Sathananthan, John Webb, David Wood
Journal: Journal of the American College of Cardiology (2024)
TCT-955 Pathology of Noncalcific Structural Valve Deterioration in Transcatheter and Surgical Aortic Bioprosthesis
Authors: Yu Sato, Sho Torii, Kazuki Aihara, Manabu Shiozaki, Norihito Nakamura, Ryousuke Omura, Junichi Miyamaoto, Norihiko Kamioka, Yohei Ohno, Gaku Nakazawa, Yuji Ikari
Journal: Journal of the American College of Cardiology (2024)
TCT-959 Validation of the Valve Academic Research Consortium High Bleeding Risk Definition in Patients Undergoing Transcatheter Aortic Valve Replacement
Authors: Marisa Avvedimento, Pedro Cepas, Julien Ternacle, Marina Urena, Alberto Alperi, Gabriela Veiga Fernández, Luis Nombela Franco, Victoria Vilalta, Giovanni Esposito, Francisco Campelo-Parada, Ciro Indolfi, Maria Del Trigo, Antonio Munoz-Garcia, Nicolas Manuel Maneiro Melon, Lluis Asmarats, Ander Regueiro, David del Val, Vincent Auffret, Melanie Cote, Roxana Mehran, Davide Capodanno, Philippe Garot, Josep Rodes-Cabau
Journal: Journal of the American College of Cardiology (2024)
TCT-927 Clinical Outcomes of Valve-in-Valve Transcatheter Aortic Valve Replacement With Newer Generation Self-Expanding Valve by the Size of Failed Bioprosthesis: A Single-Center Experience
Authors: Yoshiyuki Yamashita, Massimo Baudo, Serge Sicouri, William Gray, Basel Ramlawi
Journal: Journal of the American College of Cardiology (2024)
TCT-940 A Comparative Study of TENDYNE and Traditional Transcatheter Mitral Valve Implantation on Reverse Remodeling and Outcome
Authors: Chong Bin Lee, Hristian Hinkov, Natalia Solowjowa, Gulnara Ametova, Marian Kukucka, Volkmar Falk, Henryk Dreger, Christoph Klein, Axel Unbehaun
Journal: Journal of the American College of Cardiology (2024)
TCT-945 AltaValve System TMVR for the Treatment of Patients With Severe Mitral Regurgitation and Mitral Annular Calcification
Authors: Tsuyoshi Kaneko, Philippe Genereux, Krzysztof Wrobel, Michael Rinaldi, Thomas Modine, Vinayak Bapat, Paul Sorajja, Vlasis Ninios, James McCabe, John Saxon, Georg Nickenig
Journal: Journal of the American College of Cardiology (2024)
TCT-952 Identification and Analysis of Commissural Fibrosis of Bioprosthetic Heart Valve Explants: A Novel Mechanism of Bioprosthetic Stenosis
Authors: Hacina Gill, Althea Lai, Tucker Reed, Thomas Puehler, Georg Lutter, Karl Poon, Gilbert Tang, David Wood, John Webb, Geoffrey Payne, Shinichi Fukuhara, David Meier, Stephanie Sellers
Journal: Journal of the American College of Cardiology (2024)
Timing of Regadenoson-Induced Peak Hyperemia and the Effects on Coronary Flow Reserve
Authors: Nathan Kattapuram, Shahrad Shadman, Eric E Morgan, Charles Benton, Stacian Awojoodu, Dong-Yun Kim, Joao Ramos, Ana Barac, W Patricia Bandettini, Peter Kellman, Gaby Weissman, Marcus Carlsson
Journal: JACC. Cardiovascular imaging (2024)
Uric Acid and SGLT2 Inhibition With Empagliflozin in Heart Failure With Preserved Ejection Fraction
Authors: Wolfram Doehner, Stefan D Anker, Javed Butler, Faiez Zannad, Gerasimos Filippatos, Andrew J S Coats, João Pedro Ferreira, Ingrid Henrichmoeller, Martina Brueckmann, Elke Schueler, Stuart J Pocock, James L Januzzi, Milton Packer
Journal: JACC Heart Failure (2024)
Cholesterol, Atherosclerotic, and inflammatory Risks Among Patients With Statin Therapy
Authors: Neng Dai, Yiqing Hu, Xianglin Tang, WeiFeng Guo, Zhenyang Guo, Fan Zhou, Longjiang Zhang, Pranav Prakash Edavi, Hao Lu, Juying Qian, Junbo Ge
Journal: JACC. Cardiovascular imaging (2024)
Automated Identification of Heart Failure With Reduced Ejection Fraction Using Deep Learning-Based Natural Language Processing
Authors: Arash A Nargesi, Philip Adejumo, Lovedeep Singh Dhingra, Benjamin Rosand, Astrid Hengartner, Andreas Coppi, Simon Benigeri, Sounok Sen, Tariq Ahmad, Girish N Nadkarni, Zhenqiu Lin, Faraz S Ahmad, Harlan M Krumholz, Rohan Khera
Journal: JACC Heart Failure (2024)
Process mining in mHealth data analysis
Authors: Michael Winter, Berthold Langguth, Winfried Schlee, Rüdiger Pryss
Journal: npj Digital Medicine (2024)

This perspective article explores how process mining can extract clinical insights from mobile health data and complement data-driven techniques like machine learning. Despite technological advances, challenges such as selection bias and the complex dynamics of health data require advanced approaches. Process mining focuses on analyzing temporal process patterns and provides complementary insights into health condition variability. The article highlights the potential of process mining for analyzing mHealth data and beyond.
Antibiotic-induced loss of gut microbiome metabolic output correlates with clinical responses to CAR T-cell therapy
Authors: Rishika Prasad, Abdur Rehman, Lubna Rehman, Faezeh Darbaniyan, Viktoria Blumenberg, Maria-Luisa Schubert, Uria Mor, Eli Zamir, Sabine Schmidt, Tomo Hayase, Chia-Chi Chang, Lauren McDaniel, Ivonne Flores, Paolo Strati, Ranjit Nair, Dai Chihara, Luis E. Fayad, Sairah Ahmed, Swaminathan P. Iyer, Michael Wang, Preetesh Jain, Loretta J. Nastoupil, Jason Westin, Reetakshi Arora, Joel Turner, Fareed Khawaja, Ranran Wu, Jennifer B. Dennison, Meghan Menges, Melanie Hidalgo-Vargas, Kayla Reid, Marco L. Davila, Peter Dreger, Felix Korell, Anita Schmitt, Mark R. Tanner, Richard E. Champlin, Christopher R. Flowers, Elizabeth J. Shpall, Samir Hanash, Sattva S. Neelapu, Michael Schmitt, Marion Subklewe, Johannes Francois-Fahrmann, C. K. Stein-Thoeringer, Eran Elinav, Michael D. Jain, Eiko Hayase, Robert R. Jenq, Neeraj Y. Saini
Journal: Blood (2024)
CNS Relapse in High-Grade B-cell Lymphoma with MYC and BCL2 Rearrangements and Dark-Zone Signature-Expressing DLBCL
Authors: Waleed Alduaij, Aixiang Jiang, Diego Villa, Brett J Collinge, Susana Ben-Neriah, Merrill Boyle, Barbara Meissner, Laura K Hilton, Pedro Farinha, Graham W. Slack, Jeffrey W Craig, Alina S. Gerrie, Ciara L Freeman, Andrew J Mungall, Christian Steidl, Laurie H. Sehn, David W. Scott, Kerry J. Savage
Journal: Blood (2024)

High-grade B-cell lymphoma with MYC and BCL2 rearrangements (HGBCL-DH-BCL2), or 'double-hit lymphoma,' has been associated with a high risk of central nervous system (CNS) relapse. However, historic estimates are impacted by selection bias. We report CNS relapse rates associated with HGBCL-DH-BCL2 from a population-based cohort with complete fluorescence in situ hybridization testing, as well as diffuse large B-cell lymphoma morphology (DLBCL) tumors expressing the dark-zone gene expression signature (DZsig), which was originally derived from HGBCL-DH-BCL2. The 2-year CNS relapse risk in HGBCL-DH-BCL2 was 6.8%. CNS relapses were early, predominantly leptomeningeal (73%) and co-occurred with systemic relapse (64%). High-risk CNS International Prognostic Index (CNS-IPI) and concordant bone marrow involvement were associated with an elevated CNS relapse risk in HGBCL-DH-BCL2. The 'refined cell of origin' classification assigned 20% of DLBCL morphology tumors with germinal center B-cell-like phenotype (GCB-DLBCL) into a distinct subgroup based on DZsig expression (DZsig+). CNS relapse risk in DZsig+ (2-year: 6.4%) was independent of HGBCL-DH-BCL2 status and was further stratified by the CNS-IPI. CNS relapse in DZsig-negative GCB-DLBCL was rare (2-year risk 1.4%; P=.04 versus DZsig+) and exclusively parenchymal. Altogether, the CNS relapse risk in HGBCL-DH-BCL2 is lower than previously reported and DZsig refines risk stratification in GCB-DLBCL.
Soluble B-cell maturation antigen levels for disease monitoring in oligosecretory and nonsecretory relapsed multiple myeloma
Authors: Daisuke Ikeda, Shuichi Aikawa, Chiho Misono, Mitsuaki Oura, Fuminari Fujii, Hajime Sakuma, Masanori Toho, Atsushi Uehara, Rikako Tabata, Kentaro Narita, Masami Takeuchi, Tomohisa Watari, Yoshihito Otsuka, Kosei Matsue
Journal: Blood (2024)
Deuterated water labeling in ibrutinib-treated patients with CLL: leukemia cell kinetics correlate with IGHV, ZAP-70, and MRD
Authors: Ekaterina Kim, Shih-Shih Chen, Mariela Sivina, Hyunsoo Hwang, Xuelin Huang, Alessandra Ferrajoli, Nitin Jain, William G. Wierda, Dominik Wodarz, Nicholas Chiorazzi, Jan A. Burger
Journal: Blood (2024)
Adverse health outcomes in offspring of parents with alcohol-related liver disease: Nationwide Danish cohort study
Authors: Peter Jepsen, Joe West, Anna Kirstine Kjær Larsen, Anna Emilie Kann, Frederik Kraglund, Joanne R Morling, Colin Crooks, Gro Askgaard
Journal: PLoS Medicine (2024)

Background Parental drinking can cause harm to the offspring. A parent’s diagnosis of alcohol-related liver disease (ALD) might be an opportunity to reach offspring with preventive interventions. We investigated offspring risk of adverse health outcomes throughout life, their association with their parent’s educational level and diagnosis of ALD. Methods and findings We used nationwide health registries to identify offspring of parents diagnosed with ALD in Denmark 1996 to 2018 and age- and sex-matched comparators (20:1). We estimated the incidence rate ratios (IRRs) of hospital contacts with adverse health outcomes, overall and in socioeconomic strata. We used a self-controlled design to examine whether health outcomes were more likely to occur during the first year after the parent’s ALD diagnosis. The 60,804 offspring of parents with ALD had a higher incidence rate of hospital contacts from age 15 to 60 years for psychiatric disease, poisoning, fracture or injury, alcohol-specific diagnoses, other substance abuse, and of death than comparators. Associations were stronger for offspring with low compared to high socioeconomic position: The IRR for admission due to poisoning was 2.2 versus 1.0 for offspring of an ALD parent with a primary level versus a highly educated ALD parent. Offspring had an increased risk for admission with psychiatric disease and poisoning in the year after their parent’s ALD diagnosis. For example, among offspring whose first hospital contact with psychiatric disease was at age 13 to 25 years, the IRR in the first year after their parent’s ALD diagnosis versus at another time was 1.29 (95% CI 1.13, 1.47). Main limitation was inability to include adverse health outcomes not involving hospital contact. Conclusions Offspring of parents with ALD had a long-lasting higher rate of health outcomes associated with poor mental health and self-harm that increased shortly after their parent’s diagnosis of ALD. Offspring of parents of low educational level were particularly vulnerable. This study highlights an opportunity to reach out to offspring in connection with their parent’s hospitalization with ALD.
Epigenome-wide analysis across the development span of pediatric acute lymphoblastic leukemia: backtracking to birth
Authors: Akram Ghantous, Semira Gonseth Nusslé, Farah J. Nassar, Natalia Spitz, Alexei Novoloaca, Olga Krali, Eric Nickels, Vincent Cahais, Cyrille Cuenin, Ritu Roy, Shaobo Li, Maxime Caron, Dilys Lam, Peter Daniel Fransquet, John Casement, Gordon Strathdee, Mark S. Pearce, Helen M. Hansen, Hwi-Ho Lee, Yong Sun Lee, Adam J. de Smith, Daniel Sinnett, Siri Eldevik Håberg, Jill A. McKay, Jessica Nordlund, Per Magnus, Terence Dwyer, Richard Saffery, Joseph Leo Wiemels, Monica Cheng Munthe-Kaas, Zdenko Herceg
Journal: Molecular Cancer (2024)

Cancer is the leading cause of disease-related mortality in children. Causes of leukemia, the most common form, are largely unknown. Growing evidence points to an origin in-utero, when global redistribution of DNA methylation occurs driving tissue differentiation.
Apoliprotein E-mediated ferroptosis controls cellular proliferation in chronic lymphocytic leukemia
Authors: Federica Nardi, Rosita Del Prete, Roberta Drago, Anthea Di Rita, Francesco Edoardo Vallone, Sara Ciofini, Margherita Malchiodi, Laura Pezzella, Laura Tinti, Vittoria Cicaloni, Laura Salvini, Danilo Licastro, Aidan T. Pezacki, Christopher J. Chang, Giuseppe Marotta, Antonella Naldini, Silvia Deaglio, Tiziana Vaisitti, Alessandro Gozzetti, Monica Bocchia, Anna Kabanova
Journal: Leukemia (2024)

Unraveling vulnerabilities in chronic lymphocytic leukemia (CLL) represents a key approach to understand molecular basis for its indolence and a path toward developing tailored therapeutic approaches. In this study, we found that CLL cells are particularly sensitive to the inhibitory action of abundant serum protein, apolipoprotein E (ApoE). Physiological concentrations of ApoE affect CLL cell viability and inhibit CD40-driven proliferation. Transcriptomics of ApoE-treated CLL cells revealed a signature of redox and metal disbalance which prompted us to explore the underlying mechanism of cell death. We discover, on one hand, that ApoE treatment of CLL cells induces lipid peroxidation and ferroptosis. On the other hand, we find that ApoE is a copper-binding protein and that intracellular copper regulates ApoE toxicity. ApoE regulation tends to be lost in aggressive CLL. CLL cells from patients with high leukocyte counts are less sensitive to ApoE inhibition, while resistance to ApoE is possible in transformed CLL cells from patients with Richter syndrome (RS). Nevertheless, both aggressive CLL and RS cells maintain sensitivity to drug-induced ferroptosis. Our findings suggest a natural suppression axis that mediates ferroptotic disruption of CLL cell proliferation, building up the rationale for choosing ferroptosis as a therapeutic target in CLL and RS.
Diagnostic effectiveness of the Sport Mental Health Assessment Tool 1 supplemented with a brief clinical intake interview in a cohort of Polish elite Olympic athletes
Authors: Wojciech Waleriańczyk, Jarosław Krzywański, Joanna Gorgol, Katarzyna Konopka, Agata Kuśmierczyk, Grzegorz Lisek, Piotr Maleszka, Małgorzata Sławińska, Olga Surała, Grzegorz Więcław, Katarzyna Wójcik, Hubert Krysztofiak
Journal: British Journal of Sports Medicine (2024)

Objective Several studies have used the Sport Mental Health Assessment Tool 1 (SMHAT-1) among athletes but none have combined SMHAT-1 with a subsequent clinical intake interview. The aim of this study was to evaluate the diagnostic effectiveness of SMHAT-1 supplemented by a brief, in-person clinical intake interview in evaluating the prevalence of mental health concerns in elite athletes. Methods Sections 1 and 2 from SMHAT-1 were administered in person during the routine biannual medical check-up for Olympic athletes at the National Centre for Sports Medicine in Poland. Immediately after completing the measures, all athletes were interviewed by qualified psychologists who evaluated their mental health and provided recommendations. Results A total of 1121 elite athletes (545 female, 576 male) aged 18–53 (M=24.39, SD=5.09) were evaluated 2–8 months before the 2024 Paris Olympic Games; 72.4% scored above the triage threshold and 51% were positively screened in SMHAT-1’s section 2. Based on the subsequent mental health evaluation, only 9.5% showed signs of persistent mental health concerns and required referral to a mental health specialist. In 24.2%, their distress levels/symptoms were assessed as occasional and normal in relation to demands, and they underwent a psychoeducational intervention or were advised to consult a sports psychologist. Female athletes scored significantly higher in most questionnaires. Conclusion SMHAT-1 proved a useful addition to the routine medical check-ups for elite athletes. Extending SMHAT-1 with a brief clinical intake interview substantially elevated the efficacy of the mental health evaluation, with an additional benefit of raising mental health literacy.
Imaging mass cytometry-based characterisation of fibroblast subsets and their cellular niches in systemic sclerosis
Authors: Aleix Rius Rigau, Minrui Liang, Veda Devakumar, Ranjana Neelagar, Alexandru-Emil Matei, Andrea-Hermina Györfi, Christina Bergmann, Tim Filla, Vladyslav Fedorchenko, Georg Schett, Jörg H W Distler, Yi-Nan Li
Journal: Annals of the Rheumatic Diseases (2024)

Objectives Transcriptomic data demonstrated that fibroblasts are heterogeneous with functionally diverse subpopulations. Although fibroblasts are key effector cells of fibrotic diseases such as systemic sclerosis (SSc), they have not yet been characterised spatially at the cellular level. Here, we aimed to investigate fibroblast subpopulations using imaging mass cytometry (IMC) as a proteomic-based, spatially resolved omics approach. Methods We applied IMC to deconvolute the heterogeneity of 49 969 cells including 6501 fibroblasts at the single-cell level, to analyse their spatial distribution and to characterise their cellular niches in skin sections of patients with SSc and controls in situ. Results We identified 13 different subpopulations of fibroblasts in SSc and control skin, the proportion increases in five fibroblast subpopulations (myofibroblasts, FAP high , S1PR + , Thy1 + ;ADAM12 high ;PU.1 high and ADAM12 + ;GLI1 + fibroblasts) and decreases in three subpopulations (TFAM high , PI16 + ;FAP + and Thy1 + ;ADAM12 low fibroblasts). Several fibroblast subpopulations demonstrated spatial enrichment and altered cellular interactions in SSc. The proportion of S1PR + -fibroblast positively correlated with more extensive skin fibrosis, whereas high numbers of PI16 + ;FAP – -fibroblasts were associated with milder skin fibrosis. The frequency of aberrant cellular interaction between S1PR + and ADAM12 + ;GLI1 + -fibroblasts also positively associated with the extent of skin fibrosis in SSc. Conclusion Using IMC, we demonstrated profound changes in composition and localisation of the majority of fibroblast subpopulations in SSc skin. These findings may provide a rationale for specific targeting of deregulated fibroblast subpopulations in SSc. Quantification of S1PR + -fibroblast and PI16 + ;FAP – -fibroblasts may offer potential for patient stratification according to severity of skin fibrosis.
‘First, do no harm’: conducting research on interpersonal violence in sport
Authors: Tine Vertommen, Mary Woessner, Emma Kavanagh, Sylvie Parent, Aurélie Pankowiak, Leen Haerens, Cleo Schyvinck, Bram Constandt, Ramón Spaaij, Vidar Stevens, Annick Willem, Margo Mountjoy
Journal: British Journal of Sports Medicine (2024)