tbuckley/cabot-search huggingface dataset:
Significant and rapid stimulation of cytotoxic T-lymphocyte (CTL) responses against Her-2/neu positive ovarian cancer
Authors: Yuefei Yu, Wei Zhou, Petra Pilgrim, Everardo Cobos, Maurizio Chiriva-Internati
Journal: Clinical Cancer Research (2008)

A21    BACKGROUND:  Although immunotherapeutic interventions are promising, current treatments to induce strong immune responses against ovarian cancer (OC) are still inadequate. In order to develop a new method to induce strong immune responses against OC, we used the rAAV-based antigen loading of dendritic cells  (DCs) to generate significant and specific CTL responses against OC antigen. Recent studies demonstrate that recombinant adeno-associated  virus (rAAV)-based antigen loading of DCs generates  in vitro , significant and rapid cytotoxic T-lymphocyte (CTL) responses against viral  antigens. We used the rAAV system to induce specific CTL against tumor antigens for the development of OC gene therapy. As an extension of the versatility of the rAAV system, we  incorporated a  self -antigen, Her-2/neu, expressed in many cancers, including breast and OC. We then tested the ability of r-AAV loading  of DCs to generate CTLs specific for the self-antigen.   METHODS:  The  AAV/Her-2/neu vectors encoding a short (157-612) and long domain (1-1197) were constructed, and the titers of AAV/Her2/157 and AAV/Her2/1197 virus stocks were determined  by real-time PCR. AAV/Her2/neu-transduced DCs were  confirmed by Immunofluorescence and measuring polyadenylated RNA expression of the AAV/Her2/neu transgene. We then generated autologous targets  by infecting donor Peripheral blood  mononuclear cells (PBMCs) with AAV/Her2/157 and AAV/Her2/1197 virus 4 days  before the CTL assay. The ability of rAAV-transduced DCs to  stimulate Her2/neu-specific CTLs was determined by standard 6-hour  51 Cr assay.   RESULTS:  AAV/Her2/neu-infected PBMCs were found  to express Her2/157 and Her2/1197 by RT-PCR analysis, whereas unaltered PBMCs  and K562 cells did not express Her2/neu. Our rAAV vectors induced strong stimulation of CTLs directed against the self tumor antigen Her-2/neu. Moreover, a significant MHC Class I-restricted, anti-Her-2/neu-specific CTL killing was demonstrated against Her-2/neu positive OC cells after one  in vitro  stimulation .  The results showed that the T-cells incubated  with both AAV/Her2/157 and AAV/Her2/1197-loaded DCs were able to kill the patients’ OC cells. The CTLs’ stimulation due by Her2/1197 loaded DCs was superior to the one due by Her2/157, which confirmed our hypothesis that the Her2/1197 containing more Her2/neu-derived specific epitopes than Her2/157.   CONCLUSIONS:  In summary, our results demonstrate that the delivery of Her2/neu antigen by an AAV vector is a good strategy for generating anti-Her-2/neu CTLs. A single PBMC stimulation with AAV/Her2/157 or AAV/Her2/1197 pulsed DCs induces strong antigen specific-CTLs generation. CTLs were capable to lyse low doses of peptide pulsed into target cells or OC Her-2/neu +  tumors. These data suggest that AAV-based antigen loading of DC is highly effective for generating human CTL responses against OC antigens.
Airway inflammation is a continuous trait in children regardless of asthma symptoms
Authors: Ann-Marie M. Schoos, Bo L.K. Chawes, Klaus Bønnelykke, Hans Bisgaard
Journal: European Respiratory Journal (2011)

Rationale: Elevated fractional exhaled nitric oxide (FeNO) and bronchial hyperresponsiveness are used as surrogate markers of asthma.

Objective: To investigate the association between FeNO and bronchial responsiveness in a population of high risk children including the full spectrum from asymptomatic children to children with intermittent asthmatic symptoms and children with persistent asthma.

Methods: An unselected group of 196 six-year-old children were included from the Copenhagen Prospective Study on Asthma in Childhood (COPSAC) birth cohort born of mothers with asthma. Bronchial responsiveness was assessed as the relative change in specific airway resistance after cold dry air hyperventilation. FeNO measurements were performed prior to the hyperventilation test. The association between FeNO and bronchial responsiveness was assessed by generalized linear models.

Measurements and main results: Bronchial responsiveness and FeNO exhibited a significant and linear association. A doubling of FeNO corresponded to an 8.4% increase in airway resistance after challenge (95% CI; 3.7-13.1; p=0.0006). There was no evidence of interaction with current asthma and stratified analyses showed similar associations in children with and without asthma.

Conclusion: FeNO and bronchial responsiveness are associated and continuous traits in the population regardless of asthma. This suggests bronchial inflammation may be present subclinically, and cautions against the use of these surrogate markers for a dichotomized approach to asthma diagnosis. Childhood asthma remains a clinical diagnosis and surrogate markers may only be used cautiously as supportive evidence.
Abstract 15: Limiting GRK2 in the Ischemic Heart can Promote Cardiac Regeneration
Authors: Maria Cecilia Scimia, Lin Zuo, Kate E Sydnes, Daniel A Zuppo, Erhe Gao, Walter J Koch
Journal: Circulation Research (2014)

The detrimental role of G protein-coupled receptor (GPCR) kinase (GRK2) following cardiac injury/stress has been documented over the last two decades. Importantly, our lab has shown that inhibition or deletion of GRK2 in cardiomyocytes can prevent and also rescue heart failure (HF) phenotypes. Its role in GPCR desensitization including regulation of β-adrenergic receptors (βARs) during HF development has been well characterized. However, recently our lab and others have found that GRK2 can have novel GPCR-independent effects in the heart that appear to contribute to its pathological effects and thus, inhibition of these actions of GRK2 may contribute to therapeutic effects seen. In this study we explored whether the cardiac repair observed with lower myocardial GRK2 might involve regenerative processes. In cardiac-specific GRK2 knockout (KO) mice and also transgenic mice with cardiac-targeted expression of the βARKct, a peptide inhibitor of GRK2 activation via Gβγ sequestration, we induced HF via coronary artery ligation and subsequent myocardial infarction (MI) and measured aspects of cardiac repair including potential regeneration indices. Post-MI mice (GRK2 KO, βARKct mice and wild-type and non-transgenic control mice) were treated with 5-ethynyl-2’-deoxyuridine (EdU) or Bromodeoxyuridine (BrDU) to examine indices of DNA proliferation in myocytes as well as Ki67 staining. We also quantitated c-kit+ cells and myocytes in the post-MI hearts to compare how either loss of GRK2 expression or inhibition via its C-terminus altered potential regeneration mechanisms compared to control mice with endogenous GRK2 levels and activity. We found significantly more BrDU positive myocytes in post-MI hearts with lower GRK2 and this correlated with increased myocytes that were also cKit+. Thus, it appears that the myocardial functional improvement seen in the post-MI heart with targeted lowering of GRK2 involves, to at least a certain extent, regenerative mechanisms. This adds novel insight into the therapeutic potential of GRK2 inhibition for HF.
Affective disorders among fishermen and women exposed to indoor air pollution
Authors: Victor Umoh, Etete Peters, Gregory Erhabor, Bassey Edet, Essien Ekpe, Chidozie Adiukwu
Journal: European Respiratory Journal (2011)

Introduction:  Toxic effects of air pollution can lead to psychiatric symptoms such as anxiety and changes in mood and behaviour.   Objectives:  To examine the relationship between anxiety and depression with exposure to indoor air pollution and respiratory symptoms.   Methods:  521 fishermen and women from a fishing community in the Niger Delta region of Nigeria who were chronically exposed to firewood smoke completed the Hospital Anxiety and Depression Scale (HADS) and a modified British Medical Research Council Questionnaire (BMRC) for other respiratory symptoms. Lung volumes were measured with a spirometer.   Results:  71 (13.6%) subjects had symptoms of anxiety while 72 (13.8%) had depression and 53 (10.2%) had a combination of anxiety and depression. Anxiety symptoms was significantly more common among women. Symptoms of anxiety and depression were significantly more common among subjects with airway obstruction and subjects with chronic bronchitis. There was no significant association between symptoms of anxiety and depression and age. HADS scores correlated significantly with levels of exposure to indoor air pollution.   Conclusion:  Symptoms of anxiety and depression are common among people with long term exposure to indoor air pollution and they usually go undiagnosed due to low accessibility to proper health facilities. They should be routinely screened for psychiatric morbidities.
A Picture Is Worth a Thousand Words
Authors: David Dobarro, Maria Carmen Gómez-Rubín, Javier Fuertes-Beneitez, Angel Sánchez-Recalde, Francisco Dominguez-Melcón, Teresa López-Fernández, David Cabestrero, Maria Carmen Monedero, Mar Moreno-Yanguela, Jose Maria Mesa, Jose Luis López-Sendón
Journal: Journal of the American College of Cardiology (2010)
The use 0f two-way TV in bringing mental health services to the inner city
Authors: N Straker, P Mostyn, C Marshall
Journal: American Journal of Psychiatry (1976)

The authors describe a cable TV link between a medical school and a child health station in East Harlem. Nurse associates and community health workers trained through television conferences with a child psychiatrist have the primary responsibility for patient care at the clinic. Patients and their mothers are evaluated by the child psychiatrist in TV consultations at which nurse associates, health workers, medical students, and child psychiatric fellows are present. Patients and mothers respond positively to the system, and a high percentage of the psychiatrist's treatment recommendations are accepted. The authors suggest that such TV links can increase mental health services to underserved inner-city children.
A CASE OF A PUTRID EMPYEMA WITH A BRONCHOPLEURAL FISTULA SUCCESSFULLY TREATED WITH PENICILLIN
Authors: A L FORSGREN
Journal: Annals of Internal Medicine (1949)

Case Reports1 October 1949A CASE OF A PUTRID EMPYEMA WITH A BRONCHOPLEURAL FISTULA SUCCESSFULLY TREATED WITH PENICILLINARTHUR L. FORSGREN, M.D.ARTHUR L. FORSGREN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-31-4-691 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptRapid advances have been made in the treatment of empyema thoracis with the advent of penicillin, especially with its use intrapleurally. In all probability, the full therapeutic potentialities of penicillin have not been fully explored. The classical indications for surgical intervention in the treatment of empyema have been modified extensively by penicillin. Finland et al.1 expressed the opinion that more than one-half of all cases of empyema can be cured by repeated aspirations and local instillations of penicillin into the pleural cavity.There is general agreement that certain indications for early surgical treatment persist. Included among these indications are: the...Bibliography1. BROWNORYMEADSFINLAND BEMMM: Penicillin treatment of empyema: report of 24 cases and review of the literature, Ann. Int. Med., 1946, xxiv, 343-370. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Minneapolis, Minnesota*Received for publication March 24, 1947.From Veterans Administration Hospital, Minneapolis, Minnesota, and the Department of Medicine, University of Minnesota.Published with permission of the Chief Medical Director, Department of Medicine and Surgery, Veterans Administration, who assumes no responsibility for the opinions expressed or conclusions drawn by the author. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 October 1949Volume 31, Issue 4Page: 691-697KeywordsFistulasHospital medicinePenicillinSurgery ePublished: 1 December 2008 Issue Published: 1 October 1949 PDF downloadLoading ...
Restricting access to florbetapir: Medicare coverage criteria for diagnostics and drugs are inconsistent: Table 1
Authors: Joshua P Cohen, Jinghui Dong, Christine Y Lu, Ranjana Chakravarthy
Journal: BMJ (2015)

Medicare's decision to restrict coverage of florbetapir, a diagnostic agent for Alzheimer's disease, exemplifies an inconsistent approach to technology evaluation. <b>Joshua Cohen and colleagues</b> call for consistency
The Effects of the Spleen Tyrosine Kinase Inhibitor, Fostamatinib, on an Immune Thrombocytopenia Mouse Model
Authors: Gulsum Emel Pamuk, Mehmet Sevki Uyanik, Turan Karaca, Demirtas Selim, Muzaffer Demir, Omer Nuri Pamuk
Journal: Blood (2014)
A case of mistaken muscles
Authors: R. H Whitaker
Journal: BMJ (2002)

Editor—Ahmed and Ali wondered whether it is the oblique eye muscles or the superior or inferior recti that adduct the eye. The answer was that the obliques abduct and the recti adduct.1-1 Several clinic examination textbooks differed from this, but mine does not express a view.1-2 Gray's Anatomy was quoted as the ultimate authority.

No one knows what these muscles do in isolation. When each eye muscle functions normally the end result is a complex interaction involving six muscles. When one or more of the eye nerves or muscle(s) function less well than normal the end result is the combination of failure of the muscle(s) involved plus the unopposed action of other muscles. It is thus not possible to extract unqualified statements about the action of an individual muscle as though it functions in isolation.

What can be said is a qualified statement that when the eye is abducted the superior and inferior recti elevate or depress it, and when the eye is adducted the superior oblique depresses it and the inferior oblique elevates it (even the lateral and medial recti, if their action could be considered in isolation, would not just abduct and adduct it).
Lymphomas expressing ALK fusion protein(s) other than NPM-ALK.
Authors: Brunangelo Falini, Karen Pulford, Alessandra Pucciarini, Antonino Carbone, Chris De Wolf-Peeters, Jacqueline Cordell, Marco Fizzotti, Antonella Santucci, Pier Giuseppe Pelicci, Stefano Pileri, Elias Campo, German Ott, Georges Delsol, David Y. Mason
Journal: Blood (1999)
Milk intake and bone mineral acquisition in adolescent girls
Authors: S. New, G. Ferns, B. Starkey, I D Griffiths, R M Francis, P. Appleby, R. Eastell, J. Cadogan, N. Bright, M. E Barker
Journal: BMJ (1998)

Editor—Cadogan et al reported the effect of increased milk intake on bone mineral acquisition in English schoolgirls.1 The results of their study have important implications for childhood dietary policy. We are concerned, however, that the results may be confounded by associated changes in dietary intake. We noted comments made by a participant in the study on a BBC news item that she consumed her additional milk with extra cereal. 

Most cereals available in the United Kingdom are supplemented with a variety of vitamins and minerals. Furthermore, there is evidence in the literature that these micronutrients are important to bone health,2 and the effect observed may therefore reflect in part the additional micronutrient intake from this source. Indeed, the intake of phosphorus, magnesium, zinc, and vitamins B-1 and B-2 were higher in the milk supplemented group. This may not be solely attributable to the milk. 

Details of how the milk was consumed are not given in the paper, but the data should be available from the interim food diaries recording non-weighed food intake over four days that subjects completed on five interim occasions. It would be interesting and important for these additional data to be presented to avoid possible misinterpretation of the results.
Abstract 15918: Switching Patients with Atrial Fibrillation from Amiodarone to Dronedarone: Results of the ARTEMIS AF Studies
Authors: Gerald Naccarelli, Deepak L Bhatt, A J Camm, Jean-Yves Le Heuzey, Federico Lombardi, Juan Tamargo, Sylvie Bozzi, Catherine Marchese-Roland
Journal: Circulation (2012)

Switching a patient from one antiarrhythmic drug (AAD) to another is usually timed to minimize arrhythmia recurrence and drug-drug adverse reactions. For most AADs, this is based on stopping an AAD...
Patients who use drugs during inpatient substance abuse treatment
Authors: S F Greenfield, R D Weiss, M L Griffin
Journal: American Journal of Psychiatry (1992)

Drug use by patients during inpatient substance abuse treatment is frequently a cause of premature hospital discharge. The authors examined the histories of patients who used drugs while in inpatient substance abuse treatment, the methods used to detect drug use, and the temporal relationship of drug use episodes.The authors reviewed the charts of patients admitted consecutively to an inpatient substance abuse treatment unit between 1981 and 1988. Of 729 patients, 42 were found to have used drugs while receiving inpatient treatment. The diagnoses and clinical histories of these 42 patients were compared with those of all other patients. Methods of detection and circumstances of drug use were recorded, and drug use episodes were plotted on a time line that was examined for temporal clustering.Significantly more of the patients who used drugs during inpatient substance abuse treatment had primarily used heroin or methadone before treatment. Random urine screens, self-report, and staff observation together detected approximately 85% of the patients who used drugs during treatment. Episodes of drug use did cluster in time, but within clusters the hospital stays of the patients who used drugs did not necessarily overlap.Temporal clustering of drug use episodes may occur because drug use by one patient heralds drug availability and stimulates craving in other inpatients. Clinicians should be aware that one episode of drug use may be followed by others. Multiple detection methods, including random comprehensive urine screens, should be used to test for drug use on inpatient units.
Pro–B-Type Natriuretic Peptide1–108 Circulates in the General Community
Authors: Fima Macheret, Guido Boerrigter, Paul McKie, Lisa Costello-Boerrigter, Brian Lahr, Denise Heublein, Sharon Sandberg, Yasuhiro Ikeda, Alessandro Cataliotti, Kent Bailey, Richard Rodeheffer, John C. Burnett
Journal: Journal of the American College of Cardiology (2011)
Burden of Comorbidities and Functional and Cognitive Impairments in Elderly Patients at the Initial Diagnosis of Heart Failure and Their Impact on Total Mortality
Authors: Khalil Murad, David C. Goff, Timothy M. Morgan, Gregory L. Burke, Traci M. Bartz, Jorge R. Kizer, Sarwat I. Chaudhry, John S. Gottdiener, Dalane W. Kitzman
Journal: JACC Heart Failure (2015)
BMA annual meeting: Public health is threatened by "wholesale reductions" in staff and "dumbing down"
Authors: G. Iacobucci
Journal: BMJ (2015)

Public health medicine is under threat from the “wholesale reductions” in medically qualified jobs in local authorities and at Public Health England, the BMA has warned.

Amid claims that some local authorities were actively “dumbing down” by seeking to employ public health staff on lower pay grades, representatives at the BMA’s annual representative meeting in Liverpool this week backed a motion calling for the association to negotiate with Public …
Screening for Bacterial Vaginosis in Pregnancy to Prevent Preterm Delivery: U.S. Preventive Services Task Force Recommendation Statement
Journal: Annals of Internal Medicine (2008)

Description: Update of the 2001 U.S. Preventive Services Task Force (USPSTF) recommendation on screening for bacterial vaginosis in pregnancy. Methods: The USPSTF weighed the benefits and harms of screening for bacterial vaginosis in pregnancy by identifying new evidence addressing previously identified gaps from the 2001 USPSTF recommendation. Published literature on this topic was identified by using MEDLINE, Cochrane Library databases, the Database of Abstracts of Reviews of Effects, reference lists, and consultation with experts and was systematically reviewed. When data allowed, a series of meta-analyses (using new and 2001 report data) was done to estimate the pooled effect of treatment on preterm delivery (<37 weeks, <34 weeks, or <32 weeks) and on low birthweight and preterm, premature rupture of membranes. Recommendation: Do not screen for bacterial vaginosis in pregnant women at low risk for preterm delivery. (D recommendation) Current evidence is insufficient to assess the balance of benefits and harms of screening for bacterial vaginosis in pregnant women at high risk for preterm delivery. (I statement)
Mutation Analysis in Cystic Fibrosis
Authors: W. K. Lemna, G. L. Feldman, S. D. Fernbach, E. P. Zevkovich, W. E. O'Brien, A. L. Beaudet, F. S. Collins, B. S. Kerem, J. R. Riordan, L. C. Tsui
Journal: New England Journal of Medicine (1990)
Comparison of Stem Cell Transplantation with Reduced Intensity Conditioning (RIC) and Myeloablative Conditioning (MAC) for Children with Acute Lymphoblastic Leukemia
Authors: Koji Kato, Motohiro Kato, Daiichiro Hasegawa, Hirohide Kawasaki, Hiroyuki Ishida, Yasuhiro Okamoto, Katsuyoshi Koh, Masami Inoue, Jiro Inagaki, Keisuke Kato, Hisashi Sakamaki, Hiromasa Yabe, Keisei Kawa, Ritsuro Suzuki, Yoshiko Atsuta
Journal: Blood (2014)
Relationships of Retinal Arteriolar and Venular Diameters with Carotid Intima-Media Thickness: The Beaver Dam Offspring Study
Authors: Ayleen Godreau, Ronald Klein, Kristine Lee, Barbara E. K. Klein, Carla Schubert, Charles W. Acher, Guan-Hua Huang, F. Javier Nieto, James S. Pankow, Karen J. Cruickshanks
Journal: Circulation (2009)
Intensified concomitant chemoradiotherapy for poor-prognosis head and neck cancer.
Authors: I Alastuey
Journal: Journal of Clinical Oncology (1995)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1995.13.5.1281 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 7738633 Intensified concomitant chemoradiotherapy for poor-prognosis head and neck cancer. I AlastueyxI AlastueySearch for articles by this author https://doi.org/10.1200/JCO.1995.13.5.1281 PDF "Intensified concomitant chemoradiotherapy for poor-prognosis head and neck cancer.." Journal of Clinical Oncology, 13(5), pp. 1281–1282© 1995 by American Society of Clinical Oncology