tbuckley/cabot-search huggingface dataset:
Highly Active Frozen Nanovesicles Microneedles for Senile Wound Healing via Antibacteria, Immunotherapy, and Skin Regeneration
Authors: Jingyi Xu, Sihan Lin, Hongyan Chen, Guangzheng Yang, Mingliang Zhou, Yili Liu, Anshuo Li, Shi Yin, Xinquan Jiang
Journal: Advanced Healthcare Materials (2024)

Abstract Senile wound healing risks a variety of health complications and makes both economic and psychological burdens on patients greatly. Poor activity of aged dermal fibroblasts (A‐FBs) and local disordered immunoreaction in the deep dermis contribute to delayed wound healing. Therefore, the locally complex microenvironment in deep requires additional processing. Herein, a novel double‐layer hyaluronic acid methacrylate (HAMA)/polyvinyl alcohol (PVA) microneedle patch (MNP) coated by young fibroblast‐derived exosomes (Y‐EXOs) (Y‐EXOs@HAMA/PVA MNP) is presented for deep drug delivery, aged wound healing and immunoregulation. A spraying and freeze‐drying method is applied for keeping the bioactivity of the nanovesicles. An ideal loading of Y‐EXOs and enhanced strength for penetration have realized after circulation for times. The Y‐EXOs@HAMA/PVA MNP shows an excellent influence on delayed wound healing of aged skin with active A‐FBs, more deposition of collagen and less production of IL‐17A compared with application of aged fibroblast‐derived exosomes (A‐EXOs). Moreover, the content microRNAs in Y‐EXOs and A‐EXOs are sequenced for further study. This study initiatively demonstrates that Y‐EXOs have effective function on both anti‐aging and anti‐inflammation and Y‐EXOs@HAMA/PVA MNP is expected as a novel strategy for deep drug delivery for promoting hard wound healing in aged skin in future clinical application.
Loss of Airway Phylogenetic Diversity Is Associated with Clinical and Pathobiological Markers of Disease Development in COPD
Authors: Kristopher Opron, Lesa A. Begley, John R. Erb-Downward, Gen Li, Neil E. Alexis, Igor Barjaktarevic, R. Graham Barr, Eugene R. Bleecker, Richard Boucher, Russell P. Bowler, Stephanie A. Christenson, Alejandro P. Comellas, Gerard Criner, Christopher B. Cooper, David Couper, Craig J. Galban, MeiLan K. Han, Annette Hastie, Charles Hatt, Eric A. Hoffman, Robert J. Kaner, Mehmet Kesimer, Jerry A. Krishnan, David C. LaFon, Fernando J. Martinez, Victor E. Ortega, Stephen P. Peters, Robert Paine, Nirupama Putcha, Prescott G. Woodruff, Gary B. Huffnagle, Ariangela J. Kozik, Jeffrey L. Curtis, Yvonne J. Huang, Neil E. Alexis, Wayne H. Anderson, Mehrdad Arjomandi, Igor Barjaktarevic, R. Graham Barr, Patricia Basta, Lori A. Bateman, Christina Bellinger, Surya P. Bhatt, Eugene R. Bleecker, Richard C. Boucher, Russell P. Bowler, Russell G. Buhr, Stephanie A. Christenson, Alejandro P. Comellas, Christopher B. Cooper, David J. Couper, Gerard J. Criner, Ronald G. Crystal, Jeffrey L. Curtis, Claire M. Doerschuk, Mark T. Dransfield, M. Bradley Drummond, Christine M. Freeman, Craig Galban, Katherine Gershner, MeiLan K. Han, Nadia N. Hansel, Annette T. Hastie, Eric A. Hoffman, Yvonne J. Huang, Robert J. Kaner, Richard E. Kanner, Mehmet Kesimer, Eric C. Kleerup, Jerry A. Krishnan, Wassim W. Labaki, Lisa M. LaVange, Stephen C. Lazarus, Fernando J. Martinez, Merry-Lynn McDonald, Deborah A. Meyers, Wendy C. Moore, John D. Newell, Elizabeth C. Oelsner, Jill Ohar, Wanda K. O’Neal, Victor E. Ortega, Robert Paine, Laura Paulin, Stephen P. Peters, Cheryl Pirozzi, Nirupama Putcha, Sanjeev Raman, Stephen I. Rennard, Donald P. Tashkin, J. Michael Wells, Robert A. Wise, Prescott G. Woodruff
Journal: American Journal of Respiratory and Critical Care Medicine (2024)

The airway microbiome has the potential to shape chronic obstructive pulmonary disease (COPD) pathogenesis, but its relationship to outcomes in milder disease is unestablished.
Association of a healthy beverage score with total mortality in the adult population of Spain: A nationwide cohort study
Authors: Montserrat Rodríguez-Ayala, Carolina Donat-Vargas, Belén Moreno-Franco, Diana María Mérida, José Ramón Banegas, Fernando Rodríguez-Artalejo, Pilar Guallar-Castillón
Journal: PLoS Medicine (2024)

Background Despite the substantial evidence of the relationship between diet and mortality, the role of beverage consumption patterns is not well known. The aim of this study was to assess the association of the adherence to a Healthy Beverage Score (HBS) and all-cause mortality in a representative sample of the Spanish adult population. Methods and findings We conducted an observational cohort study using data from the Study on Nutrition and Cardiovascular Risk in Spain (ENRICA), which included 12,161 community-dwelling individuals aged ≥18 years recruited in 2008 to 2010 and followed until January 2022. At baseline, food consumption was collected using a validated diet history. The HBS consists of 7 items, each of which is scored from 1 to 4 (highest adherence). The HBS ranges from 7 to 28 points with a higher score representing a healthier pattern. Adherence was assigned as a higher consumption of low-fat milk, and coffee and tea, a lower consumption of whole-fat milk, no consumption of fruit juice, artificially sweetened beverages, or sugar-sweetened beverages, and no or moderate consumption of alcohol. Total mortality was ascertained by linkage to the Spanish National Death Index. Statistical analyses were performed with Cox models and adjusted for the main confounders, including sociodemographic, lifestyle, dietary variables, and morbidity. After a mean follow-up of 12.5 years (SD: 1.7; range: 0.5 to 12.9), a total of 967 deaths occurred. For all-cause mortality, the fully adjusted hazard ratio (HR) for the highest versus lowest sex-specific quartiles of HBS was 0.72 (95% confidence interval [0.57, 0.91], p linear-trend = 0.015), corresponding to an 8.3% reduction in the absolute risk of death. A linear relationship between the risk of death and the adherence to the HBS was observed using restricted cubic splines. The results were robust to sensitivity analyses. The main limitation was that repeated measurements on beverage consumption were not available and beverage consumption could have changed during follow-up. Conclusions In this study, we observed that higher adherence to the HBS was associated with lower total mortality. Adherence to a healthy beverage pattern could play a role in the prevention of premature mortality.
Extracorporeal Carbon Dioxide Removal to Avoid Invasive Ventilation During Exacerbations of Chronic Obstructive Pulmonary Disease: VENT-AVOID Trial – A Randomized Clinical Trial
Authors: Abhijit Duggal, Steven A. Conrad, Nicholas A. Barrett, Mohamed Saad, Tariq Cheema, Sonal Pannu, Ramiro Saavedra Romero, Laurent Brochard, Stefano Nava, V. Marco Ranieri, Alexandra May, Daniel Brodie, Nicholas S. Hill, Tiffany Dumont, Sudhir Krishnan, Matthew Exline, Francisco Paz, Joshua Huelster, Boris Medarov, Juan Sanchez, Michael Benninghoff, Matthew Barrett, Ivor Douglas, Jason Mansoori, Craig Rackley, Neil MacIntyre, Christian Bermudez, William Schweickert, Christopher King, Mehul Desai, Jennifer Rovella, Rita Pechulis, L. Keith Scott, Muthiah P. Muthiah, Cara Agerstrand, Darryl Abrams, Alexis Serra, Mangala Narasimham, Eric Gottesman, Mitchell Levy, Sabiha Hussain, Sugeet Jagpal, Maximiliano Tamae-Kakazu, Stephen Fitch, Gerald J. Criner, Parag Desai, Anthony Faugno, Brian M. Morrissey, Timothy E. Albertson, Mindaugas Rackauskas, Kevin Doerschug, Jigme Sethi, Kyle Enfield, Alexandra Kadl, Bindu Akkanti, Kha Dinh, Asif Saberi, Ashish Mehta, Raghu Seethala, James Blum, Mark Williams, Ketan Buch, Si Pham, James Walter, William Tillis, Robert Balk, Zafar Jamkhana, Pratibha Kaul, Duncan Hite, Muhanned Abu-Hijleh, Faisal Siddiqui, Tracey Dill, Yuki Kinoshita, Laura Lund
Journal: American Journal of Respiratory and Critical Care Medicine (2024)

It is unclear whether extracorporeal CO
The new WHO 2022 and ICC proposals for the classification of myelodysplastic neoplasms. Validation based on the Düsseldorf MDS Registry and proposals for a merged classification
Authors: K. Nachtkamp, C. Strupp, M. Vukelja, A. Kasprzak, D. Haase, C. Ganster, B. Hildebrandt, B. Betz, A. Giagounidis, C. Aul, S. Blum, W. K. Hofmann, M. Pfeilstöcker, P. Valent, M. Lübbert, M. Seidl, M. Rudelius, R. Stauder, O. Krieger, K. S. Götze, J. Bobak, A. Kündgen, F. Schulz, S. Dietrich, G. Kobbe, N. Gattermann, U. Germing
Journal: Leukemia (2024)
The Kir2.1E299V mutation increases atrial fibrillation vulnerability while protecting the ventricles against arrhythmias in a mouse model of short QT syndrome type 3
Authors: Ana I Moreno-Manuel, Álvaro Macías, Francisco M Cruz, Lilian K Gutiérrez, Fernando Martínez, Andrés González-Guerra, Isabel Martínez Carrascoso, Francisco José Bermúdez-Jimenez, Patricia Sánchez-Pérez, María Linarejos Vera-Pedrosa, Juan Manuel Ruiz-Robles, Juan A Bernal, José Jalife
Journal: Cardiovascular Research (2024)

Short QT syndrome type 3 (SQTS3) is a rare arrhythmogenic disease caused by gain-of-function mutations in KCNJ2, the gene coding the inward rectifier potassium channel Kir2.1. We used a multidisciplinary approach and investigated arrhythmogenic mechanisms in an in-vivo model of de-novo mutation Kir2.1E299V identified in a patient presenting an extremely abbreviated QT interval and paroxysmal atrial fibrillation.
Single-dose azithromycin for infant growth in Burkina Faso: Prespecified secondary anthropometric outcomes from a randomized controlled trial
Authors: Ali Sié, Mamadou Ouattara, Mamadou Bountogo, Clarisse Dah, Thierry Ouedraogo, Valentin Boudo, Elodie Lebas, Huiyu Hu, Benjamin F. Arnold, Kieran S. O’Brien, Thomas M. Lietman, Catherine E. Oldenburg
Journal: PLoS Medicine (2024)

Background Antibiotic use during early infancy has been linked to childhood obesity in high-income countries. We evaluated whether a single oral dose of azithromycin administered during infant-well visits led to changes in infant growth outcomes at 6 months of age in a setting with a high prevalence of undernutrition in rural Burkina Faso. Methods and findings Infants were enrolled from September 25, 2019, until October 22, 2022, in a randomized controlled trial designed to evaluate the efficacy of a single oral dose of azithromycin (20 mg/kg) compared to placebo when administered during well-child visits for prevention of infant mortality. The trial found no evidence of a difference in the primary endpoint. This paper presents prespecified secondary anthropometric endpoints including weight gain (g/day), height change (mm/day), weight-for-age Z-score (WAZ), weight-for-length Z-score (WLZ), length-for-age Z-score (LAZ), and mid-upper arm circumference (MUAC). Infants were eligible for the trial if they were between 5 and 12 weeks of age, able to orally feed, and their families were planning to remain in the study area for the duration of the study. Anthropometric measurements were collected at enrollment (5 to 12 weeks of age) and 6 months of age. Among 32,877 infants enrolled in the trial, 27,298 (83%) were followed and had valid anthropometric measurements at 6 months of age. We found no evidence of a difference in weight gain (mean difference 0.03 g/day, 95% confidence interval (CI) −0.12 to 0.18), height change (mean difference 0.004 mm/day, 95% CI −0.05 to 0.06), WAZ (mean difference −0.004 SD, 95% CI −0.03 to 0.02), WLZ (mean difference 0.001 SD, 95% CI −0.03 to 0.03), LAZ (mean difference −0.005 SD, 95% CI −0.03 to 0.02), or MUAC (mean difference 0.01 cm, 95% CI −0.01 to 0.04). The primary limitation of the trial was that measurements were only collected at enrollment and 6 months of age, precluding assessment of shorter-term or long-term changes in growth. Conclusions Single-dose azithromycin does not appear to affect weight and height outcomes when administered during early infancy. Trial registration ClinicalTrials.gov NCT03676764
Synchronous Pulmonary Arterial and Pulmonary Venous Thrombosis in Pancreatic Cancer
Authors: Anant Jain, Oleg Epelbaum
Journal: American Journal of Respiratory and Critical Care Medicine (2024)

"Synchronous Pulmonary Arterial and Pulmonary Venous Thrombosis in Pancreatic Cancer." American Journal of Respiratory and Critical Care Medicine, 209(6), pp. 751–752
Without progressive reforms, the future of Chile’s healthcare system remains bleak
Authors: Sebastián Villarroel González, Juan V A Franco, Camila Micaela Escobar Liquitay
Journal: BMJ (2024)

The prospect of progressive reform of Chile's health system is uncertain after a recent vote to reject a proposed new constitution, write <b>Sebastián Villarroel González</b>, <b>Juan V A Franco</b>, and <b>Camila Micaela Escobar Liquitay</b>
Circulating immunotherapy strategy based on pyroptosis and STING pathway: Mn-loaded paclitaxel prodrug nanoplatform against tumor progression and metastasis
Authors: Bingjie Wang, Teng Wang, Tianze Jiang, Shuang Li, Lianxiao Zhang, Xia Zhao, Xiaojia Yang, Xueyang Wang
Journal: Biomaterials (2024)
Epigenetic reprogramming as a key to reverse ageing and increase longevity
Authors: Beatriz Pereira, Francisca P. Correia, Inês A. Alves, Margarida Costa, Mariana Gameiro, Ana P. Martins, Jorge A. Saraiva
Journal: Ageing Research Reviews (2024)

The pursuit for the fountain of youth has long been a fascination amongst scientists and humanity. Ageing is broadly characterized by a cellular decline with increased susceptibility to age-related diseases, being intimately associated with epigenetic modifications. Recently, reprogramming-induced rejuvenation strategies have begun to greatly alter longevity research not only to tackle age-related defects but also to possibly reverse the cellular ageing process. Hence, in this review, we highlight the major epigenetic changes during ageing and the state-of-art of the current emerging epigenetic reprogramming strategies leveraging on transcription factors. Notably, partial reprogramming enables the resetting of the ageing clock without erasing cellular identity. Promising chemical-based rejuvenation strategies harnessing small molecules, including DNA methyltransferase and histone deacetylase inhibitors are also discussed. Moreover, in parallel to longevity interventions, the foundations of epigenetic clocks for accurate ageing assessment and evaluation of reprogramming approaches are briefly presented. Going further, with such scientific breakthroughs, we are witnessing a rise in the longevity biotech industry aiming to extend the health span and ideally achieve human rejuvenation one day. In this context, we overview the main scenarios proposed for the future of the socio-economic and ethical challenges associated with such an emerging field. Ultimately, this review aims to inspire future research on interventions that promote healthy ageing for all.
Exposure to Nitrogen Dioxide and Fine Particulate Matter When Cooking with Electricity Compared to Gas, a Randomized Crossover Study in Quito, Ecuador
Authors: Carlos F. Gould, Lissete Davila, M. Lorena Bejarano, Marshall Burke, Darby W. Jack, Samuel B. Schlesinger, José R. Mora, Alfredo Valarezo
Journal: Environmental Health Perspectives (2024)
“Real-World” Evidence, Target Trial Emulation, and Randomized Clinical Trials—Which Data Should Clinicians Rely on When Choosing Antibiotics?
Authors: David L. Paterson, Helmi bin Sulaiman
Journal: JAMA Network Open (2024)

Sandra Tingsgård, MD; Simone Bastrup Israelsen, MD, PhD; Henrik Løvendahl Jørgensen, MD, PhD; Christian Østergaard, MD, DMSc; Thomas Benfield, MD, DMSc
Dysregulated immune and metabolic pathways are associated with poor survival in adult acute myeloid leukemia with CEBPA bZIP in-frame mutations
Authors: Feng-Ming Tien, Chi-Yuan Yao, Xavier Cheng-Hong Tsai, Min-Yen Lo, Chien-Yuan Chen, Wan-Hsuan Lee, Chien-Chin Lin, Yuan-Yeh Kuo, Yen-Ling Peng, Mei-Hsuan Tseng, Yu-Sin Wu, Ming-Chih Liu, Liang-In Lin, Ming-Kai Chuang, Bor-Sheng Ko, Ming Yao, Jih-Luh Tang, Wen-Chien Chou, Hsin-An Hou, Hwei-Fang Tien
Journal: Blood Cancer Journal (2024)

Acute myeloid leukemia (AML) with CEBPA bZIP in-frame mutations (CEBPAbZIP-inf) is classified within the favorable-risk group by the 2022 European LeukemiaNet (ELN-2022). However, heterogeneous clinical outcomes are still observed in these patients. In this study, we aimed to investigate the mutation profiles and transcriptomic patterns associated with poor outcomes in patients with CEBPAbZIP-inf. One hundred and thirteen CEBPAbZIP-inf patients were identified in a cohort of 887 AML patients homogeneously treated with intensive chemotherapy. Concurrent WT1 or DNMT3A mutations significantly predicted worse survival in AML patients with CEBPAbZIP-inf. RNA-sequencing analysis revealed an enrichment of interferon (IFN) signaling and metabolic pathways in those with a shorter event-free survival (EFS). CEBPAbZIP-inf patients with a shorter EFS had higher expression of IFN-stimulated genes (IRF2, IRF5, OAS2, and IFI35). Genes in mitochondrial complexes I (NDUFA12 and NDUFB6) and V (ATP5PB and ATP5IF1) were overexpressed and were associated with poorer survival, and the results were independently validated in the TARGET AML cohort. In conclusion, concurrent WT1 or DNMT3A mutations and a dysregulated immune and metabolic state were correlated with poor survival in patients with CEBPAbZIP-inf, and upfront allogeneic transplantation may be indicated for better long-term disease control.
Mental Health Symptoms When Abortion Access Is Restricted
Authors: Julia R. Steinberg
Journal: JAMA (2024)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Patient Self-Assessment of Walking Ability and Fracture Risk in Older Australian Adults
Authors: Dana Bliuc, Thach Tran, Dunia Alarkawi, Weiwen Chen, Dima A. Alajlouni, Fiona Blyth, Lyn March, Robert D. Blank, Jacqueline R. Center
Journal: JAMA Network Open (2024)

Importance The relationship between self-reported walking limitation, a proxy of muscle function, and fracture risk has not been investigated. Objective To examine the association between a self-reported walking limitation of 1000 m or less and 5-year risk of fracture. Design, Setting, and Participants This prospective cohort study compared individuals with various degrees of walking ability limitation at 1000 m (a little limitation and a lot of limitation) and those without limitation (no limitation) accounting for age, falls, prior fractures, and weight. Participants from the ongoing population-based Sax Institute 45 and Up Study were followed from recruitment (2005-2008) for 5 years (2010-2013). Data analysis was conducted from July 2020 to September 2023. Exposure Self-reported walking limitation. Main Outcomes and Measures Incident fracture and site-specific fractures (hip, vertebral, and nonhip nonvertebral [NHNV] fractures). Results Among the 266 912 participants enrolled in the 45 and Up Study, 238 969 were included, with 126 015 (53%) women (mean [SD] age, 63 [11] years) and 112 954 (47%) men (mean [SD] age, 61 [11] years). Approximately 20% reported a degree of limitation in walking 1000 m or less at baseline (39 324 women [24%]; 23 191 men [21%]). During a mean (SD) follow-up of 4.1 (0.8) years, 7190 women and 4267 men experienced an incident fracture. Compared with participants who reported no walking limitations, a little limitation and a lot of limitation were associated with higher risk of fracture (a little limitation among women: hazard ratio [HR], 1.32; 95% CI, 1.23-1.41; a little limitation among men: HR, 1.46; 95% CI, 1.34-1.60; a lot of limitation among women: HR, 1.60; 95% CI, 1.49-1.71; a lot of limitation among men: HR, 2.03; 95% CI, 1.86-2.22). Approximately 60% of fractures were attributable to walking limitation. The association was significant for hip, vertebral, and NHNV fracture and ranged between a 21% increase to a greater than 219% increase. Conclusions and Relevance In this cohort study of 238 969 participants, self-reported walking limitations were associated with increased risk of fracture. These findings suggest that walking ability should be sought by clinicians to identify high-risk candidates for further assessment.
Outcomes After Minimally Invasive Surfactant Therapy in Preterm Infants
Authors: Shen Ko, Shin-Hwa Tsai, Nin-Chieh Hsu
Journal: JAMA (2024)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Phosphorylation of pyruvate dehydrogenase inversely associates with neuronal activity
Authors: Dong Yang, Yu Wang, Tianbo Qi, Xi Zhang, Leyao Shen, Jingrui Ma, Zhengyuan Pang, Neeraj K. Lal, Daniel B. McClatchy, Saba Heydari Seradj, Verina H. Leung, Kristina Wang, Yi Xie, Filip S. Polli, Anton Maximov, Oscar Christian Gonzalez, Luis de Lecea, Hollis T. Cline, Vineet Augustine, John R. Yates, Li Ye
Journal: Neuron (2024)

For decades, the expression of immediate early genes (IEGs) such as FOS has been the most widely used molecular marker representing neuronal activation. However, to date, there is no equivalent surrogate available for the decrease of neuronal activity. Here, we developed an optogenetic-based biochemical screen in which population neural activities can be controlled by light with single action potential precision, followed by unbiased phosphoproteomic profiling. We identified that the phosphorylation of pyruvate dehydrogenase (pPDH) inversely correlated with the intensity of action potential firing in primary neurons. In in vivo mouse models, monoclonal antibody-based pPDH immunostaining detected activity decreases across the brain, which were induced by a wide range of factors including general anesthesia, chemogenetic inhibition, sensory experiences, and natural behaviors. Thus, as an inverse activity marker (IAM) in vivo, pPDH can be used together with IEGs or other cell-type markers to profile and identify bi-directional neural dynamics induced by experiences or behaviors.