Is detection of enteropathogens and human or animal faecal markers in the environment associated with subsequent child enteric infections and growth: an individual participant data meta-analysis
Authors: Andrew Mertens, Benjamin F Arnold, Jade Benjamin-Chung, Alexandria B Boehm, Joe Brown, Drew Capone, Thomas Clasen, Erica R Fuhrmeister, Jessica A Grembi, David Holcomb, Jackie Knee, Laura H Kwong, Audrie Lin, Stephen P Luby, Rassul Nala, Kara Nelson, Sammy M Njenga, Clair Null, Amy J Pickering, Mahbubur Rahman, Heather E Reese, Lauren Steinbaum, Jill R Stewart, Ruwan Thilakaratne, Oliver Cumming, John M Colford, Ayse Ercumen
Journal: The Lancet Global Health (2024)

BackgroundQuantifying contributions of environmental faecal contamination to child diarrhoea and growth faltering can illuminate causal mechanisms behind modest health benefits in recent water, sanitation, and hygiene (WASH) trials. We aimed to assess associations between environmental detection of enteropathogens and human or animal microbial source tracking markers (MSTM) and subsequent child health outcomes.MethodsIn this individual participant data meta-analysis we searched we searched PubMed, Embase, CAB Direct Global Health, Agricultural and Environmental Science Database, Web of Science, and Scopus for WASH intervention studies with a prospective design and concurrent control that measured enteropathogens or MSTM in environmental samples, or both, and subsequently measured enteric infections, diarrhoea, or height-for-age Z-scores (HAZ) in children younger than 5 years. We excluded studies that only measured faecal indicator bacteria. The initial search was done on Jan 19, 2021, and updated on March 22, 2023. One reviewer (AM) screened abstracts, and two independent reviewers (AM and RT) examined the full texts of short-listed articles. All included studies include at least one author that also contributed as an author to the present Article. Our primary outcomes were the 7-day prevalence of caregiver-reported diarrhoea and HAZ in children. For specific enteropathogens in the environment, primary outcomes also included subsequent child infection with the same pathogen ascertained by stool testing. We estimated associations using covariate-adjusted regressions and pooled estimates across studies.FindingsData from nine published reports from five interventions studies, which included 8603 children (4302 girls and 4301 boys), were included in the meta-analysis. Environmental pathogen detection was associated with increased infection prevalence with the same pathogen and lower HAZ (ΔHAZ –0·09 [95% CI –0·17 to –0·01]) but not diarrhoea (prevalence ratio 1·22 [95% CI 0·95 to 1·58]), except during wet seasons. Detection of MSTM was not associated with diarrhoea (no pooled estimate) or HAZ (ΔHAZ –0·01 [–0·13 to 0·11] for human markers and ΔHAZ –0·02 [–0·24 to 0·21] for animal markers). Soil, children's hands, and stored drinking water were major transmission pathways.InterpretationOur findings support a causal chain from pathogens in the environment to infection to growth faltering, indicating that the lack of WASH intervention effects on child growth might stem from insufficient reductions in environmental pathogen prevalence. Studies measuring enteropathogens in the environment should subsequently measure the same pathogens in stool to further examine theories of change between WASH, faecal contamination, and health. Given that environmental pathogen detection was predictive of infection, programmes targeting specific pathogens (eg, vaccinations and elimination efforts) can environmentally monitor the pathogens of interest for population-level surveillance instead of collecting individual biospecimens.FundingThe Bill & Melinda Gates Foundation and the UK Foreign and Commonwealth Development Office.