tbuckley/cabot-search huggingface dataset:
Evidence of Immunogenic Cell Death (ICD) and ICD-dependent Dendritic Cell Activation Induced by Extracorporeal Photopheresis in Patients with leukemic forms of cutaneous T cell lymphoma
Authors: Angelika Lackner, Teresa Burner, Marlene Brandner, Saptaswa Dey, Stefan Aigner, Veronika Buxhofer-Ausch, Marija Geroldinger-Simic, Christoph Iselin, Yun-Tsan Chang, Yi-Chien Tsai, Sabine Altrichter, Peter Wolf, Susanne Kimeswenger, Emmanuella Guenova, Wolfram Hoetzenecker
Journal: British Journal of Dermatology (2025)

Despite novel therapeutic options, the long-term management of cutaneous T cell lymphoma (CTCL) remains challenging. Extracorporeal photopheresis (ECP) is an immunomodulating photochemotherapy associated with higher overall survival when used for the treatment of leukemic forms of CTCL. Its exact mode of action is not fully elucidated. Immunogenic cell death (ICD) is pivotal in cancer immunotherapy, marked by the release of damage-associated molecular patterns that enhance dendritic cell (DC) maturation and cytotoxic T lymphocyte responses. This study explores ICD in patients with leukemic forms of CTCL during ECP and its effect on DC activation. We conducted in vitro studies with peripheral blood mononuclear cells (PBMCs) from healthy donors and ex vivo experiments with white blood cells (WBCs) from patients with leukemic forms of CTCL undergoing ECP. We assessed cell viability, apoptosis, and ICD markers (ATP, HMGB1, calreticulin) using flow cytometry, ELISA, and qPCR. Engulfment assays evaluated DC activation by ECP-treated CD4+ T cells. ECP-treated healthy PBMCs and WBCs from patients with leukemic forms of CTCL showed significant induction of ICD hallmarks, including ATP release, HMGB1 secretion, and calreticulin surface exposure. In the patients with leukemic forms of CTCL, calreticulin expression was mainly present in CD4+CD26- T cells, indicating greater ICD susceptibility of malignant T cells. ECP-treated CD4+ T cells were phagocytosed by DCs, a process which we found to be dependent on ICD signals. ECP induces ICD in malignant T cells and, to a lesser extent, in healthy T cells, facilitating DC activation. These findings suggest that ECP enhances targeted immune responses against malignant T cells in leukemic forms of CTCL, offering new insights into its therapeutic mechanisms and potential applications in cancer immunotherapy.
A qualitative interview study exploring patient views and experiences of treatment for hidradenitis suppurativa in the UK
Authors: Laura Howells, Paul Leighton, Kim S Thomas, Fiona Collier, Angela Gibbons, Ceri Harris, Kerry Hood, Muhammad Riaz, Jeremy Rodrigues, Helen Stanton, Emma Thomas-Jones, John R Ingram
Journal: British Journal of Dermatology (2025)

Hidradenitis suppurativa (HS) is a long-term skin condition where evidence for management after first line treatment fails is limited, and practice varies across the UK. Both medical and surgical treatment options are potential avenues for treatment. Furthermore, patient perspectives on HS treatments have received little attention in research to date. To explore patients' views and experiences of treatment for HS to inform clinical care. A nested qualitative study within a prospective cohort study. Interviews with 35 participants were completed by telephone. Purposive sampling was undertaken. Framework analysis was used to develop themes. Views on treatments: Past experiences and knowledge informed patient beliefs and whether an individual felt a treatment option was appropriate or a good 'fit' for them at a specific moment in time. Healthcare professional recommendations can be influential on both patient's views and which treatment option they ultimately receive. Experiences of treatments: There were positive experiences across all treatment types used within the study. Negative experiences included side effects of medications, lack of efficacy, delays to procedures, and burden of wound care. However, even when personal experiences were not wholly positive for an individual, participants often believed the same treatment may potentially help others with HS, due to the importance placed on personalisation of treatment. This paper has implications for how healthcare professionals discuss treatment options with people with HS. A 'one size fits all' approach is inappropriate, and shared decision making that elicits patient beliefs and preferences is crucial.
Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity
Authors: Nicholas J Tursi, Sachchidanand Tiwari, Nicole Bedanova, Toshitha Kannan, Elizabeth Parzych, Nisreen Okba, Kevin Liaw, András Sárközy, Cory Livingston, Maria Ibanez Trullen, Ebony N Gary, Máté Vadovics, Niklas Laenger, Jennifer Londregan, Mohammad Suhail Khan, Serena Omo-Lamai, Hiromi Muramatsu, Kerry Blatney, Casey Hojecki, Viviane Machado, Igor Maricic, Trevor R F Smith, Laurent M Humeau, Ami Patel, Andrew Kossenkov, Jacob S Brenner, David Allman, Florian Krammer, Norbert Pardi, David B Weiner
Journal: Cell Reports Medicine (2025)
Effectiveness of nasal high-flow oxygen during apnoea on hypoxaemia and intubation success in paediatric emergency and ICU settings: a randomised, controlled, open-label trial
Authors: Shane George, Tara Williams, Susan Humphreys, Tiffany Atkins, David Tingay, Ben Gelbart, Trang Pham, Simon Craig, Simon Erickson, Arjun Chavan, Katie Rasmussen, Anusha Ganeshalingham, Felix Oberender, Subodh Ganu, Nitesh Singhal, Kristen Gibbons, Renate Le Marsney, Juerg Burren, Luregn J Schlapbach, Brenda Gannon, Mark Jones, Stuart R Dalziel, Andreas Schibler
Journal: The Lancet Respiratory Medicine (2025)
New opportunities for accessing promising non-oncological orphan drugs
Authors: Carla E M Hollak, Carelle C Reparon-Schuijt, Kathelijn Verdeyen, Lisa J Deesker, Jaap W Groothoff, Josephine M I Vos, Atse H Huisman, Silvia van der Flier, Anke A M G Pisters-van Roy
Journal: The Lancet Regional Health - Europe (2025)
Metal-Free Antioxidant Nanozyme Incorporating Bioactive Hydrogel as an Antioxidant Scaffold for Accelerating Bone Reconstruction
Authors: Yang Yang, Qianrui Zeng, Chaoyue Zhao, Jie Shi, Wanmeng Wang, Yunkai Liang, Changyi Li, Qingxin Guan, Bo Chen, Wei Li
Journal: Biomaterials (2025)
A horizon scan for Arctic coastal biodiversity research: understanding changes requires international collaboration
Authors: Jakob Thyrring, Philippe Archambault, Michael Burrows, Katrin Iken, Fernando P Lima, Joana Micael, Markus Molis, Catia Monteiro, Sergej Olenin, Paul E Renaud, Ricardo A Scrosati, Rui Seabra, Alexey A Sukhotin, Jan-Marcin Węsławski, Nadescha Zwerschke, Mikael K Sejr
Journal: Trends in Ecology & Evolution (2025)
Awake replay: off the clock but on the job
Authors: Matthijs A A van der Meer, Daniel Bendor
Journal: Trends in Neurosciences (2025)

Hippocampal replay is widely thought to support two key cognitive functions: online decision-making and offline memory consolidation. In this review, we take a closer look at the hypothesized link between awake replay and online decision-making in rodents, and find only marginal evidence in support of this role. By contrast, the consolidation view is bolstered by new computational ideas and recent data, suggesting that (i) replay performs offline fictive learning for later goal-oriented behavior; and (ii) replay tags memories prior to sleep, prioritizing them for consolidation. Based on these recent advances, we favor an updated and refined role for awake replay - that is, supporting prioritized offline learning and tagging outside the hippocampus - rather than a direct, online role in guiding behavior.
A role for respiration in coordinating sleep oscillations and memory consolidation
Authors: Fabian Schwimmbeck, Esteban Bullón Tarrasó, Thomas Schreiner
Journal: Trends in Neurosciences (2025)

Memory consolidation is thought to rely on the interplay of sleep-related brain oscillations. Drawing on recent findings that highlight the influence of respiration on these rhythms, we outline a framework positioning respiration as pacemaker for sleep's memory function. By orchestrating the cardinal non-rapid eye movement (NREM) oscillations, namely slow oscillations, spindles, and sharp wave-ripples, respiration may coordinate the hippocampo-cortical crosstalk essential for memory consolidation.
Corrigendum to “Two decades of continuous progresses and breakthroughs in the field of bioactive ceramics and glasses driven by CICECO-hub scientists” [Bioact. Mater. 40 (2024) 104–147]
Authors: H R Fernandes, S Kannan, M Alam, G E Stan, A C Popa, R Buczyński, P Gołębiewski, J M F Ferreira
Journal: Bioactive Materials (2025)
Wearable AI to enhance patient safety and clinical decision-making
Authors: Arjun Mahajan, Kimia Heydari, Dylan Powell
Journal: npj Digital Medicine (2025)

Wearable artificial intelligence (AI) technologies show promise in healthcare, with early applications demonstrating diverse benefits for patient safety. These systems go beyond traditional data collection, using advanced algorithms to provide real-time clinical guidance. From infectious disease monitoring to AI-powered surgical assistance, these technologies enable proactive, personalized care while addressing critical safety gaps. However, successful implementation requires careful consideration of technical, operational, and ethical challenges.
A call to action to address the steatotic liver disease public health threat in Barcelona
Authors: Jeffrey V Lazarus, J Emilio Miralles-Sanchez, Leire Agirre-Garrido, Nicolai Brachowicz, Aina Nicolàs, Anthony Armenta, Delfina Boudou, Lisa Rice-Duek, Juan M Pericàs, Joan Benach, Christopher J Kopka, Dana Ivancovsky Wajcman
Journal: The Lancet Regional Health - Europe (2025)
Tumor-Infiltrating Lymphocyte Scoring Improves Progression Risk Prediction in Stage II Melanoma: A Retrospective Cohort Study
Authors: George Adigbli, Benedict Reed, Bhavika Khera, Mahaveer Sangha, Simran Thadani, Annika Wilder-Smith, Milosz Wojtowicz, Mary Pissaridou, Ameer Mustafa, Eleni Ieremia, Olivia Espinosa, Jonathan Dunne, Fadi Issa, Oliver Cassell
Journal: Journal of the American Academy of Dermatology (2025)

AJCC 8th edition substaging might be suboptimal for predicting melanoma progression. Using it to select stage II patients for adjuvant immunotherapy risks overtreating low-risk stage IIB/IIC patients and undertreating high-risk stage IIA patients. Prognostic capability of tumor-infiltrating lymphocytes (TILs) is unclear in stage II melanoma. To evaluate AJCC substaging and TIL scoring as predictors of progression in stage II melanoma. Retrospective cohort study of 366 SLN(-) stage II melanoma patients from four UK hospitals (2004-2017), with long-term follow-up. 23% of melanomas progressed (median 9.5-year follow-up). Among those, 41.5% were stage IIA, 41.5% IIB, and 17.1% IIC. TIL scoring independently predicted progression risk (non-brisk vs brisk: OR 0.298,p=0.009; non-brisk vs absent: OR 0.436,p=0.049) and PFS. Non-brisk TILs, present in 80% of progressing tumors, denoted high risk. TIL scoring split patients into high and low risk across substages: stage IIA patients with non-brisk TILs had similar 5-year PFS to stage IIB/IIC patients with absent/brisk TILs. Retrospective study design and unknown generalizability. Stage II melanoma progression is poorly predicted by AJCC 8 substage. TIL scoring offers improved risk stratification across substages and could serve as a cost-effective method to better identify patients who may benefit from adjuvant immunotherapies.
Combined AAV-mediated specific Gjb2 expression restores hearing in DFNB1 mouse models
Authors: Qiuhan Sun, Fangzhi Tan, Liyan Zhang, Yicheng Lu, Hao Wei, Nianci Li, Lulu Jiang, Yinyi Zhou, Tian Chen, Ling Lu, Geng-Lin Li, Jieyu Qi, Shiming Yang, Renjie Chai
Journal: Molecular Therapy (2025)
Supramolecular assembly of multi-purpose tissue engineering platforms from human extracellular matrix
Authors: Bruno Ladeira, Maria Gomes, Kongchang Wei, Catarina Custódio, João Mano
Journal: Biomaterials (2025)

Recapitulating the biophysical and biochemical complexity of the extracellular matrix (ECM) remains a major challenge in tissue engineering. Hydrogels derived from decellularized ECM provide a unique opportunity to replicate the architecture and bioactivity of native ECM, however, they exhibit limited long-term stability and mechanical integrity. In turn, materials assembled through supramolecular interactions have achieved considerable success in replicating the dynamic biophysical properties of the ECM. Here, we merge both methodologies by promoting the supramolecular assembly of decellularized human amniotic membrane (hAM), mediated by host-guest interactions between hAM proteins and acryloyl-β-cyclodextrin (AcβCD). Photopolymerization of the cyclodextrins results in the formation of soft hydrogels that exhibit tunable stress relaxation and strain-stiffening. Disaggregation of bulk hydrogels yields an injectable granular material that self-reconstitutes into shape-adaptable bulk hydrogels, supporting cell delivery and promoting neovascularization. Additionally, cells encapsulated within bulk hydrogels sense and respond to the biophysical properties of the surrounding matrix, as early cell spreading is favored in hydrogels that exhibit greater susceptibility to applied stress, evidencing proper cell-matrix interplay. Thus, this system is shown to be a promising substitute for native ECM in tissue repair and modelling.
Endothelial but not systemic ferroptosis inhibition protects from antineutrophil cytoplasmic antibody-induced crescentic glomerulonephritis
Authors: Anthony Rousselle, Dörte Lodka, Janis Sonnemann, Lovis Kling, Ralph Kettritz, Adrian Schreiber
Journal: Kidney International (2025)

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) are systemic autoimmune diseases featuring small blood vessel inflammation and organ damage, including necrotizing crescentic glomerulonephritis (NCGN). Persistent vascular inflammation leads to endothelial and kidney cell necrosis. Ferroptosis is a regulated cell death pathway executed by reactive oxygen species and iron-dependent lipid peroxidation culminating in cell membrane rupture. Here we show that ANCA-activated neutrophils induced endothelial cell (EC) death in vitro that was prevented by ferroptosis inhibition with Ferrostatin-1, Liproxstatin-1 and small inhibiting RNA against the enzyme AcylCoA Synthetase Long Chain Family Member 4 (ACSL4). In contrast, neither necroptosis nor apoptosis inhibition affected EC death. Moreover, both ferroptosis inhibitors alleviated lipid peroxide accumulation in EC. Increased lipid peroxidation was detected in kidney sections of AAV mice by immunohistochemistry. We generated MPO-/- ACSL4flox Tie2-Cre+ mice lacking ACSL4 specifically in EC (ACSL4ΔEC) to study the significance of endothelial ferroptosis in vivo. ACSL4ΔEC chimeric mice, but not control mice (ACSL4WT), were protected from NCGN in a MPO-AAV bone-marrow transplantation model. These data establish that EC ferroptosis contributes to ANCA-induced glomerulonephritis. However, systemic pharmacological ferroptosis inhibition with Ferrostatin-1 or Liproxstatin-1 did not protect from NCGN in a murine AAV model. Ferrostatin-1 treatment both directly activated T cell proliferation and indirectly myeloid-mediated T cell proliferation and polarization in vitro. Conceivably, both effects may cancel the beneficial effect of endothelial ferroptosis inhibition. Mechanistically, we describe the importance of EC ferroptosis for the development of AAV. However, the lack of protection with systemic pharmacological ferroptosis inhibition should discourage clinicians from evaluating this treatment strategy in clinical AAV studies.
Life-supporting functional kidney replacement by integration of embryonic metanephros-bladder composite tissue transplants
Authors: Yoshitaka Kinoshita, Eiji Kobayashi, Kenji Matsui, Yuka Inage, Keita Morimoto, Shutaro Yamamoto, Satomi Iwai, Kento Kitada, Kentaro Iwasawa, Yatsumu Saito, Toshinari Fujimoto, Kei Matsumoto, Shushi Nagamori, Akira Nishiyama, Haruki Kume, Takanori Takebe, Takashi Yokoo, Shuichiro Yamanaka
Journal: Kidney International (2025)

Novel transplantable organs need to be developed to address the global organ shortage. Transplantation of embryonic kidney tissue, or metanephros, facilitates glomerular and tubular maturation and offers partial organ functional support. However, adult environments do not permit exponential growth in size, limiting the life-supporting functionality and organ replacement effect of this approach. Here, we developed a novel strategy that combines the fusion of embryonic bladders with multiple anastomoses to the host ureter, enabling a significant increase in metanephros transplantation and urinary tract integration. By surgically anastomosing divided bladder segments, we reconstructed the excretory pathways by merging four metanephroi into each bladder and integrating them with the host ureter. Following the transplantation and integration of 20 metanephroi at the para-aortic region, anephric rats survived for over a month and generated approximately 50,000 nephrons in vivo. Ultrastructural and single-cell- transcriptomic analyses revealed that the maturity of the transplanted metanephroi was comparable to that of adult kidneys, although their small size likely contributed to their decreased urine concentration ability. Postoperative support helped normalize physiological homeostasis, including solute clearance, acid-base balance, electrolyte levels, and kidney hormone levels, within vital ranges. Our findings underscore the functional maturation capacity and dose-dependent therapeutic efficacy of embryonic kidney tissue, suggesting its potential as a transplantable organ system.
Age- and sex-specific reference values of estimated glomerular filtration rates for European adults
Authors: Megan E Astley, Nicholas C Chesnaye, Stein Hallan, Giovanni Gambaro, Alberto Ortiz, Juan-Jesus Carrero, Natalie Ebert, Bjørn Odvar Eriksen, Anne-Laure Faucon, Pietro Manuel Ferraro, Olafur S Indridason, Till Ittermann, Arnar J Jonsson, Knut Asbjørn Rise Langlo, Toralf Melsom, Elke Schäeffner, Sylvia Stracke, Vianda S Stel, Kitty J Jager
Journal: Kidney International (2025)

Kidney function, often assessed by estimated glomerular filtration rate (eGFR), declines naturally with age. However, there is a lack of eGFR reference values to describe normal and abnormal values for a specific age. The European Chronic Kidney Disease Burden Consortium is comprised of nine participating general population-based studies from seven European countries which provides European age- and sex-specific eGFR reference values in healthy adults using the European Kidney Function Consortium (EKFC) equation. Of 2,572,020 individuals, 1,535,253 (60%) were considered healthy, of which 45% were men. Ages ranged from 18 to 105 years old in men and 18 to 107 years old in women with a median age of 43 years in both sexes. At age 20 in men, the 5th, 50th and 95th eGFR percentiles were 78 ml/min per 1.73 m2, 99 ml/min per 1.73 m2, and 119 ml/min per 1.73 m2. In 20-year-old women this was 81 ml/min per 1.73 m2, 101 ml/min per 1.73 m2, and 121 ml/min per 1.73 m2. Consequently, in men aged 80 years old, the 5th, 50th and 95th eGFR percentiles were 49 ml/min per 1.73 m2, 66 ml/min per 1.73 m2, and 84 ml/min per 1.73 m2. In 80 year old women this was 46 ml/min per 1.73 m2, 63 ml/min per 1.73 m2, and 81 ml/min per 1.73 m2. Overall, our study shows that eGFR is not preserved with ageing in healthy individuals and these eGFR reference values can help determine abnormal and normal kidney function across the age range.
Engineered vesicular cancer vaccines for immunosuppressive microenvironment reversion and in situ vaccine generation
Authors: Yang Li, Yulin Yu, Bin Xia, Siyu Zhao, Xiaonan Li, Qian Hu, Yinmei Tian, Yi Wang, Yixuan Zhou, Conglian Yang, Dan Zhang, Zhiping Zhang, Li Kong
Journal: Journal of Controlled Release (2025)
A combined “eat me/don’t eat me” strategy based on exosome for acute liver injury treatment
Authors: Wei Du, Chen Chen, YingYing Liu, Huiyi Quan, Ming Xu, JingJing Liu, Ping Song, ZhiQiang Fang, ZhenSheng Yue, Hao Xu, YuWei Ling, JuanLi Duan, Fei He, Lin Wang
Journal: Cell Reports Medicine (2025)
Glycemia Assessed by Continuous Glucose Monitoring among People Treated with Maintenance Dialysis
Authors: Ian H. de Boer, Lisa D. Anderson, Nathaniel K. Ashford, Ernest Ayers, Nisha Bansal, Yoshio N. Hall, Irl B. Hirsch, Andrew N. Hoofnagle, Simon Hsu, Evelin Jones, Benjamin Lidgard, Christine P. Limonte, Lori J. Linke, Chris C. Marnell, Laura Mayeda, Elizabeth McNamara, Rajnish Mehrotra, Anne Pesenson, Julie M. Porter, Matthew B. Rivara, Glenda V. Roberts, Beth Shanaman, Subbulaxmi Trikudanathan, Suzanne Watnick, Katy G. Wilkens, Leila R. Zelnick
Journal: Journal of the American Society of Nephrology (2025)

Background: Kidney failure and its treatments disrupt glucose homeostasis in ways that may promote both hyperglycemia and hypoglycemia. Continuous glucose monitoring (CGM) delineates detailed glycemic profiles, but published studies in kidney failure are limited to small, select groups. We aimed to characterize the spectrum of glycemia and its determinants in a large, diverse maintenance dialysis population. Methods: We conducted a prospective community-based cohort study of people treated with maintenance dialysis. Each participant wore a Dexcom G6 Pro CGM for approximately 10 days. Outcomes ascertained by CGM included mean blood glucose, time in range (TIR, 70-180 mg/dL), and hypoglycemia events (sustained &lt;70 mg/dL). Results: We enrolled 420 demographically diverse participants, including 263 with diabetes (of whom 88 were untreated with glucose-lowering medications) and 157 without diabetes. Peritoneal dialysis was used by 55. Outcomes varied by diabetes status and dialysis modality. Among participants without diabetes, mean blood glucose was higher with peritoneal dialysis vs hemodialysis (141 vs 121 mg/dL, p&lt;0.001). Among participants with untreated diabetes, mean blood glucose was 162 mg/dL, mean TIR 71%, and only 64% of participants attained TIR ≥70%, while mean hemoglobin A1c was 5.7%. Among participants with treated diabetes, mean blood glucose was 214 mg/dL, mean TIR 43%, and only 22% of participants attained TIR ≥70%, while mean hemoglobin A1c was 7.0%. 714 unique sustained hypoglycemia events were observed, with highest rates for participants without diabetes. In addition to diabetes and dialysis modality, age, dialysis vintage, insulin use, hemoglobin A1c, and serum albumin were significantly associated with mean blood glucose, hypoglycemia, or both. Conclusions: In maintenance dialysis, CGM frequently identifies both hyperglycemia and hypoglycemia that may not be clinically evident. In particular, hyperglycemia is common with peritoneal dialysis, patients with untreated diabetes maintain a diabetic glycemic profile, and patients with treated diabetes rarely meet contemporary CGM-based treatment targets.
Microglial NOX2 as a therapeutic target in traumatic brain injury: Mechanisms, Consequences, and Potential for Neuroprotection
Authors: Nargis Bano, Sameera Khan, Shakir Ahamad, Nawab John Dar, Hamad H Alanazi, Aamir Nazir, Shahnawaz Ali Bhat
Journal: Ageing Research Reviews (2025)
Advanced Biomarkers: Beyond Amyloid and Tau: Emerging Non-Traditional Biomarkers for Alzheimer’s Diagnosis and Progression
Authors: Meher Rijwana Afrin, Pankaj Ghritakousik Upadhyaya, Abdul Hashim, Kunal Bhattacharya, Nongmaithem Randhoni Chanu, Dibyajyoti Das, Pukar Khanal, Satyendra Deka
Journal: Ageing Research Reviews (2025)