tbuckley/cabot-search huggingface dataset:
Abstract 697: Mobilization of Oct-4 <sup>+</sup> Bone Marrow-Derived Very Small Embryonic-Like Stem Cells in Patients with Acute Myocardial Infarction
Authors: Wojciech Wojakowski, Magda Kucia, Boguslaw Machalinski, Edyta Paczkowska, Joanna Ciosek, Maciej Kazmierski, Pawel Buszman, Piotr Wieczorek, Janina Ratajczak, Mariusz Z Ratajczak, Michal Tendera
Journal: Circulation (2007)

Bone marrow-derived CD34 + CXCR4 + progenitor cells are mobilized into peripheral blood early in acute myocardial infarction (MI). Adult murine bone marrow contains population of small CD34 + lin − CD45 − CXCR4 + cells expressing markers of pluripotent stem cells (PSC) SSEA, Oct-4 and Nanog. This population of very small embryonic-like cells (VSEL) has unique morphology (small size 2– 4 μm, large nucleus, euchromatin) and capability to form embrioid bodies (EB). Murine EB-derived cells can in vitro differentiate into cells from all three germ layers including cardiomyocytes. We hypothesized that in patients with acute MI small cells expressing the VSEL immunophenotype and PSC markers are present in bone marrow and mobilized into peripheral blood. Blood samples (20 mL) from 18 patients with acute MI were obtained after 12 hours, 2 and 5 days after symptoms onset. Bone marrow samples (20 mL) were obtained from 2 patients with acute MI and 3 healthy volunteers. Mononuclear cells were isolated using hypotonic lysis and samples were analyzed by FACS. Mobilization of following cell populations was confirmed: hematopoietic lin − CD45 + CXCR4 + , lin − CD45 + CD133 + , lin − CD45 + CD34 + and non-hematopoietic (VSEL) lin − CD45 − CXCR4 + , lin − CD45 − CD133 + , lin − CD45 − CD34 + . Analysis of the cell number using lymphocyte gate showed more significant increase of CD45 + (hematopoietic) populations of lin − CD34 + , lin − CD133 + and lin − CXCR4 + cells. After gating for small events (VSEL size range) we found more significant mobilization of small, non-hematopoietic populations of lin − CD34 + , lin − CD133 + and lin − CXCR4 + cells (Table ). The expression of PSC markers (Oct-4, Nanog, SSEA-1) in VSEL was confirmed using real-time RT-PCR. Conclusion: We report for the first time that acute MI is associated with mobilization of non-hematopoietic VSELs expressing pluripotent stem cells markers.
A multi-country Salmonella Enteritidis phage type 14b outbreak associated with eggs from a German producer: ‘near real-time’ application of whole genome sequencing and food chain investigations, United Kingdom, May to September 2014
Authors: T Inns, C Lane, T Peters, T Dallman, C Chatt, N McFarland, P Crook, T Bishop, J Edge, J Hawker, R Elson, K Neal, G  K Adak, P Cleary, None on behalf of the Outbreak Control Team
Journal: Eurosurveillance (2015)

We report an outbreak of Salmonella Enteritidis phage type 14b (PT14b) in the United Kingdom (UK) between May and September 2014 where Public Health England launched an investigation to identify the source of infection and implement control measures. During the same period, outbreaks caused by a Salmonella Enteritidis strain with a specific multilocus variable-number tandem repeat analysis (MLVA) profile occurred in other European Union Member States. Isolates from a number of persons affected by the UK outbreak, who had initially been tested by MLVA also shared this particular profile. Cases were defined as any person infected with S. Enteritidis PT14b, resident in England or Wales and without history of travel outside of this geographical area during the incubation period, reported from 1 June 2014 onwards, with a MLVA profile of 2–11–9-7–4-3–2-8–9 or a single locus variant thereof. In total, 287 cases met the definition. Food traceback investigations in the UK and other affected European countries linked the outbreaks to chicken eggs from a German company. We undertook whole genome sequencing of isolates from UK and European cases, implicated UK premises, and German eggs: isolates were highly similar. Combined with food traceback information, this confirmed that the UK outbreak was also linked to a German producer.
Phase I clinical study on docetaxel/zoledronic acid combination administered in two different sequences in hormone refractory prostate cancer patients
Authors: R. V. Iaffaioli, G. Facchini, M. Caraglia, G. S. Bruni, P. Maiolino, G. Nasti, M. Marra, S. R. Addeo, L. Gallo, A. Budillon
Journal: Journal of Clinical Oncology (2007)

15646 Background: Docetaxel (DTX) has been proposed in the treatment of hormone-refractory prostate carcinoma (HRPC) as single agent and the combination between DTX and prednisone and that one between DTX and estramustine has been reported to give a higher survival as compared with the combination between mitoxanthrone and estramustine. Zoledronic acid (ZOL) is an important therapeutic weapon in the treatment of the patients affected by advanced hormone refractory prostate cancer (HRPC). Our preliminary data on prostate cancer cells in vitro suggest that ZOL and DTX are synergistic on the growth inhibition of these cells and the synergism is sequence-dependent. Prostate cancer is, therefore, a suitable target for a pharmacological combination between DTX and ZOL. On the basis of these considerations, a phase I trial on the combination between ZOL and DTX was designed in the treatment of HRPC. Methods: Phase I study with a dose-escalation DTX in combination with a defined and fixed dose of ZOL (2 mg), both administered every 14 days. The following two different sequences of administration were, moreover, explored: Sequence A: DTX at the day 1 followed by ZOL at the day 2. Sequence B: ZOL at the day 1 followed by DTX at the day 2. The first dose level of DTX was 30 mg/m 2 with a programmed dose escalation of 10 mg/m 2 for each level until 50 mg/m 2 . Sear and PBMC were also collected prior and after the different treatments at each cycle. Results: Up to date the MTD was not achieved. We have reached the second level (40 mg/m2) without recording any treatment related toxicities. Interestingly one of the 2 sequences induced a less number of haematological side effects and absence of gastrointestinal effects. A different pattern of modulation of circulating angiogenic factors (interleukin 8 and 12, VEGF, PDGF), cytokines (TNF alpha, IFN gamma, interleukin-6 and 4) and gamma/delta T lymphocyte subpopulation is recording in the 2 sequences of administration. Conclusions: The study is still ongoing and further results will be presented at ASCO meeting. No significant financial relationships to disclose.
Medicine in Persia
Authors: Cyril Elgood
Journal: JAMA (1934)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Retinal Venous Thrombosis. A Clinical Study of Sixty-Two Cases Followed over Many Years.
Authors: R. Foster Moore
Journal: JAMA (1924)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Re-Education; an Analysis of the Institutional System of the United States.
Authors: George Edward Barton
Journal: JAMA (1918)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Phase II study of weekly cisplatin, etoposide and irinotecan (PE/CPT) for refractory relapsed small cell lung cancer (SCLC)
Authors: Y. Kim, K. Goto, Y. Nishiwaki, K. Kubota, H. Omatsu, T. Tamura, Y. Ohe, H. Kunitoh, I. Sekine, N. Yamamoto, N. Saijo
Journal: Journal of Clinical Oncology (2006)

7088 Background: There is no standard treatment for relapsed SCLC. Irinotecan and etoposide has been studied in several phase II trials as active agents for relapsed SCLC. We reported the efficacy and tolerability of irinotecan combined with weekly cisplatin and etoposide (PE) in patients (pts) with sensitive relapsed SCLC (Goto, et al. Br J Cancer 2005). Methods: We conducted a phase II study to evaluate the efficacy and toxicity of PE/CPT regimen in pts with refractory relapsed SCLC, who had relapsed within 8 weeks after completion of first-line therapy. Pts must have measurable or assessable disease, age of 75 years or younger, performance status of 0 to 2 (ECOG), and adequate organ function. PE/CPT regimen consisted of cisplatin 25 mg/m 2 on day 1 at one-week intervals for 9 weeks (at least 6 weeks), etoposide 60 mg/m 2 on days 1–3 on week 1, 3, 5, 7, 9 (at least on week 1, 3, 5), and irinotecan 90 mg/m 2 on day 1 on weeks 2, 4, 6, 8 (at least on week 2, 4, 6). After day 1 on week 2, G-CSF was administered on days when cytotoxic drugs were not given. Results: From May 2000 to January 2005, 30 pts were enrolled in this study. Pt characteristics were median age 64 years (range 44–75); 23 male and 7 female; 3 LD and 27 ED. Prior chemotherapy included PE in 13 pts, cisplatin and irinotecan in 7, carboplatin and etoposide in 5, and others in 5. 23 pts (77%) completed 6 or more weeks of chemotherapy. 2 CR and 19 PR were observed and the overall response rate was 70% (95% CI 50.6–85.3%). Grade 3/4 neutropenia, anemia and thrombocytopenia were observed in 60%, 73% and 47%, respectively. Grade 3/4 diarrhea was observed in only 7%. Coclusions: PE/CPT regimen was active and well tolerated for refractory relapsed SCLC. No significant financial relationships to disclose.
miRNA-embedded shRNAs for Lineage-specific BCL11A Knockdown and Hemoglobin F Induction
Authors: Swaroopa Guda, Christian Brendel, Raffaele Renella, Peng Du, Daniel E Bauer, Matthew C Canver, Jennifer K Grenier, Andrew W Grimson, Sophia C Kamran, James Thornton, Helen de Boer, David E Root, Michael D Milsom, Stuart H Orkin, Richard I Gregory, David A Williams
Journal: Molecular Therapy (2015)

RNA interference (RNAi) technology using short hairpin RNAs (shRNAs) expressed via RNA polymerase (pol) III promoters has been widely exploited to modulate gene expression in a variety of mammalian cell types. For certain applications, such as lineage-specific knockdown, embedding targeting sequences into pol II-driven microRNA (miRNA) architecture is required. Here, using the potential therapeutic target BCL11A, we demonstrate that pol III-driven shRNAs lead to significantly increased knockdown but also increased cytotoxcity in comparison to pol II-driven miRNA adapted shRNAs (shRNA(miR)) in multiple hematopoietic cell lines. We show that the two expression systems yield mature guide strand sequences that differ by a 4 bp shift. This results in alternate seed sequences and consequently influences the efficacy of target gene knockdown. Incorporating a corresponding 4 bp shift into the guide strand of shRNA(miR)s resulted in improved knockdown efficiency of BCL11A. This was associated with a significant de-repression of the hemoglobin target of BCL11A, human γ-globin or the murine homolog Hbb-y. Our results suggest the requirement for optimization of shRNA sequences upon incorporation into a miRNA backbone. These findings have important implications in future design of shRNA(miR)s for RNAi-based therapy in hemoglobinopathies and other diseases requiring lineage-specific expression of gene silencing sequences.
Rapid, two-stage arterial switch for transposition of the great arteries and intact ventricular septum beyond the neonatal period.
Authors: Jonas Ra, Giglia Tm, Sanders Sp, Wernovsky G, Nadal-Ginard B, Mayer Je, Castaneda Ar
Journal: Circulation (1989)

Optimal management of dextrotransposition of the great arteries with intact ventricular septum is currently an arterial switch procedure performed in the first 2 weeks of life. However, a subgroup of patients presents for surgery beyond this time for reasons of sickness, size, or late referral. Experience with 11 such patients (mean age at first-stage procedure, 4.5 months) has revealed that the left ventricle can be prepared by a surprisingly short interval period (median, 9 days) between a first-stage preparatory operation (pulmonary artery band with or without a shunt) and a subsequent second-stage arterial switch procedure. Serial two-dimensional echocardiography showed that left ventricular mass increased by a mean of 85% during this short interval. Mean left ventricular-right ventricular-pressure ratio as measured by cardiac catheterization increased from 0.5 +/- 0.08 a median of 7 days before the first stage to 1.04 +/- 0.29 a median of 7 days after the first stage. One patient underwent a Senning procedure because of an intramural left coronary artery. The other 10 patients underwent an arterial switch, with no early deaths. Median hospitalization after the arterial switch was 8 days. There has been one late death at 5 months. No patient has been detected to have abnormal ventricular function, although trivial to mild aortic regurgitation has been commonly observed with color flow mapping. These results have encouraged us to offer a two-stage arterial switch procedure to appropriate infants with an interval period of approximately 1 week. Both stages can be performed at one hospitalization, with important psychosocial, logistic, and financial advantages.
What We Are and Why: A Study with Illustrations, of the Relation of the Endocrine Glands to Human Conduct and Dispositional Traits, with Special Reference to the Influence of Gland Derangements on Behavior
Authors: Laurence Hampson Mayers, Arthur Dorman Welton
Journal: JAMA (1934)

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Abstract 855: White-Coat Effect among Older Patients with Suspected Cognitive Impairment: Prevalence and Clinical Implications
Authors: Mario Bo, Massimiliano Massaia, Chiara Merlo, Alessia Brescianini, Alessandro Sona, Antonella Canadè, Gianfranco Fonte
Journal: Circulation (2008)

OBJECTIVES To evaluate prevalence of the white-coat effect (WCE), and its association with individual anxiety and insight of disease, among older patients attending for a first visit for suspected cognitive impairment (CI). DESIGN Prospective cohort study SETTING An Alzheimer Evaluation Unit. PARTICIPANTS Outpatients aged 55 years or older with suspected CI MEASUREMENTS WCE was defined as a difference of at least 20 mmHg in systolic or 10 mmHg in diastolic blood pressure (BP) measured either by a physician during the visit or by a nurse (before and after the visit), compared with home self blood pressure measurement (SBPM). Severity of CI was evaluated through the Mini-Mental State Examination (MMSE); the Clinical Insight Rating Scale (CIR) and the Guidelines for the Rating for Awareness Deficits (GRAD) were used to evaluate the subject’s insight; anxiety disorder was evaluated using the 7-question Generalized Anxiety Disorder scale (GAD-7) RESULTS Among 273 subjects, prevalence of WCE was 52%, 32.6% and 30.4%, according to physician’s and nurse’s BP measurements, respectively (p=.000). Prevalence of WCE did not differ between patients diagnosed with and without dementia, but was higher among patients with than in those without anxiety disorder (70.7% vs 38.2%, p=.000). Positive relations were observed between severity of anxiety and insight of disease, which were both inversely related with severity of CI CONCLUSIONS WCE is extremely common and is roughly correlated to individual anxiety and insight of disease among older outpatients with suspected CI; overestimation of hypertension severity might lead to unnecessary drug treatment and greater health costs in this setting.
Treatment Of Serious Bleeds With a B-Domain Deleted Recombinant Porcine Sequence Factor VIII (OBI-1) In Patients With Acquired Hemophilia A: A Prospective Clinical Trial
Authors: Rebecca Kruse-Jarres, Jean St. Louis, Anne Greist, Amy D. Shapiro, Hedy Smith, Pratima Chowdary, Anja B Drebes, Edward D. Gomperts, Miranda Chapman, Min Mo, Aaron Novack, Heinrich D. Farin
Journal: Blood (2013)
Abstract 4790: Transcatheter Off-pump Aortic Valve Implantation In Patients With Very High Risk For Conventional Aortic Valve Replacement
Authors: Matthias Thielmann, Daniel Wendt, Philipp Kahlert, Holger Eggebrecht, Evas Kottenberg-Assenmacher, Alexander Janosi, Thomas Buck, Juergen Peters, Raimund Erbel, Heinz Jakob, Stephan Sack
Journal: Circulation (2008)
Correlation of thymidylate synthase genotype and protein expression and association with lung cancer survival.
Authors: T. A. Boyle, J. Haney, R. Montoya, D. Merrick, D. R. Camidge, W. A. Franklin
Journal: Journal of Clinical Oncology (2011)

e21137 Background: Thymidylate synthase (TS) catalyzes DNA synthesis and is directly inhibited by pemetrexed, a drug used for treatment of lung cancer. The aim of this study is to analyze the association of a TS genetic polymorphism and protein expression with survival in patients with non-small cell lung cancer. Methods: We performed a retrospective study of 114 patients with NSCLC using paraffin embedded tumor tissue at the University of Colorado. DNA was extracted from each tumor specimen and assessed by fragment analysis for genotype in the TS promoter variable tandem repeat region (VNTR). Thymidylate synthase protein expression was evaluated with immunohistochemistry and percent nuclear positivity was assessed. Survival analysis with the log rank test statistic was performed to compare survival time based on TS genotype and protein expression. The association between TS genotype and protein expression was evaluated by Pearson correlation analysis. Results: Of the 114 lung tumor specimens, 32 were homozygous for three repeats (3R/3R), 49 were heterozygous (2R/3R) and 33 were homozygous for two repeats (2R/2R). Patients with lung cancer who were homozygous or heterozygous for three repeats in the TS VNTR survived significantly longer than patients homozygous for two repeats (N=114, p=0.007) with death due to lung cancer as an endpoint. Lower than average thymidylate synthase protein expression (<=12% nuclear positivity) was significantly associated with longer survival (N=94, p=0.001). The TS genotype was correlated with TS protein expression (N=94, p=0.03). Conclusions: This work suggests that TS VNTR may be an important prognostic marker in NSCLC. The TS VNTR genotype and TS protein expression are associated with a significant difference in mortality from lung cancer. The TS VNTR genotype may be associated with survival because of its association with a differential expression of TS protein. Tumors homozygous or heterozygous for three repeats in the TS VNTR are associated with lower TS expression and have a better prognosis than tumors homozygous for two repeats in the TS VNTR. Whether TS VNTR predicts response to pemetrexed is a question that warrants further study.
Abstract 12978: Incident Atrial Fibrillation is Similar in Blacks and Whites With Congestive Heart Failure
Authors: Parin J Patel, Yuliya Borovskiy, Anthony Killian, Francis E Marchlinski, Rajat Deo
Journal: Circulation (2014)

Introduction: Clinical studies have demonstrated that blacks have a lower prevalence and incidence of atrial fibrillation (AF) compared with whites. Given the strong biologic associations between AF and congestive heart failure (CHF), we hypothesized that the racial disparity for incident AF is attenuated in CHF patients. Methods: The University of Pennsylvania Atrial Fibrillation Free-Congestive Heart Failure (PAFF-CHF) Cohort is a large, multi-hospital retrospective cohort of individuals with clinical CHF and without AF at index visit. Baseline demographic and clinical parameters were obtained, and medical records were queried for incident outcomes. The primary outcome was incident AF, which was defined as a clinical or ECG diagnosis of AF on any follow up encounter. Results: Of 5,131 patients in PAFF-CHF, there were 2,037 blacks (40%) and 3,094 whites (60%). Median follow up time was 4.5 years (1.8, 5.9), with blacks having significantly longer follow up (4.7 v 4.2 yr, p &lt; 0.001). During this follow-up, 851 subjects (16%) developed AF, with rates of 5.1 per 100 person years in whites and 4.9 per 100 person years in blacks (p = 0.8). Time independent risk factors for developing AF included male gender (OR 1.42 [95% CI 1.22 - 1.65], p &lt; 0.001); LBBB on index ECG (1.32 [1.01 - 1.72], p = 0.04); and black race (OR 1.31 [1.13 - 1.52], p &lt; 0.001). In a regression model of traditional risk factors for AF including age, gender, hypertension, coronary artery disease, diabetes, and renal insufficiency, black race remained an independent risk factor for AF (OR 1.45 [1.23 - 1.70], p &lt; 0.001). Finally, time to event analysis showed no difference in freedom from AF and no difference in freedom from AF or death (Figure). Conclusions: In a large cohort of HF patients, incident AF was similar in blacks and whites. Although prior studies have indicated a low prevalence and incidence of AF in blacks compared with whites, the development of AF appears to be a common finding in both races after a diagnosis of CHF.