tbuckley/cabot-search huggingface dataset:
Abstract 1308: Mobilization Of Murine Endothelial Progenitor Cells Requires Medullary Fibroblast Growth Factor-2
Authors: Nikos Werner, Vincent 1/4aine, Tilman Preiss, Jean Francois Arnal, Georg Nickenig
Journal: Circulation (2007)

Background Fibroblast growth factor-2 (FGF2) regulates proliferation of bone marrow (BM) stromal cells and has pro-angiogenic effects regulating endothelial cell growth, migration, and reendothelialization. We have previously demonstrated that estrogen-mediated endothelial progenitor cell (EPC) mobilization from BM into peripheral blood is inhibited in FGF2 knock-out (−/−) mice indicating a central role of medullary FGF2 in progenitor cell mobilization. Here we determine the underlying molecular mechanisms of FGF2-dependent statin-mediated progenitor cell mobilization. Methods and Results To determine the molecular mechanisms of FGF2-mediated progenitor cell mobilization, FGF2−/− and wild type (wt) mice were treated with the HMG-CoA reductase-inhibitor rosuvastatin (10mg/kg body weight s.c. per day) and placebo. In contrast to wt mice, the number of Sca1/flk-1 positive EPC in peripheral blood of rosuvastatin treated FGF2−/− mice did not increase. This was due to an accumulation of Sca1/flk-1 positive cells within the BM. Inhibition of EPC mobilization in FGF2−/− mice was associated with a delay in reendothelialization in a mouse model of focal endothelial cell denudation and a severe reduction in neoangiogenesis despite statin treatment. BM transplantation experiments demonstrated that reconstitution of FGF2−/− mice with wt stem cells completely restored statin-mediated EPC mobilization while wt mice reconstituted with FGF2−/− stem cells developed a mobilization defect associated with an impaired endothelial-dependent vasore-laxation and enhanced neointima formation. In wt mice, statin treatment was associated with an increased number of activated osteoclasts compared to placebo treated mice which are known to play a pivotal role in stem cell mobilization. Conclusion Medullary FGF2 is essential in the statin-mediated mobilization of EPC from BM into peripheral blood while it seems not to influence EPC proliferation. The mobilization defect in FGF2−/− mice is associated with a significant impairment of vascular endothelial function and repair which underlines the importance of EPC in endothelial cell regeneration.
Abstract 6205: Variants of CYP4V2, SERPINC1 and GP6 Are Associated with Pulmonary Embolism
Authors: Lance A Bare, Irene D Bezemer, Carmen H Tong, Andre R Arellano, Charles M Rowland, Joseph J Catanese, Pieter H Reitsma, Carine J Doggen, James J Devlin, Frits R Rosendaal
Journal: Circulation (2008)

Background: Pulmonary Embolism is a potentially fatal manifestation of venous thrombosis that may share genetic risk factors with deep vein thrombosis (DVT). Recently, 3 single nucleotide polymorphisms (SNPs) were reported to be associated with DVT in three studies: rs13146272 in CYP4V2 , rs2227589 in SERPINC1 , rs1613662 in GP6 . We investigated whether these 3 SNPs were also associated with pulmonary embolism. Methods: We genotyped 5840 subjects from the Multiple Environmental and Genetic Assessment of risk factors for venous thrombosis (MEGA study). Cases were 1,206 patients who had a pulmonary embolism without a symptomatic DVT: isolated pulmonary embolism (PE iso ). Controls were 4,634 subjects who had no history of DVT or pulmonary embolism. We tested the association between genotypes and PE iso using logistic regression models that adjusted for age and sex. Results: For all three SNPs, the same allele that had been previously found to be associated with increased risk of DVT was also associated with increased risk of PE iso . For homozygous carriers of the risk allele, compared with noncarriers, the ORs were 1.34 (95% CI, 1.08–1.67) for rs13146272 in CYP4V2 , 1.85 (95% CI, 1.08–3.16) for rs2227589 in SERPINC1 , and 1.20 (95% CI, 1.04–1.39) for rs1613662 in GP6. The frequencies of risk allele homozygotes in the controls of the MEGA study were 41% for rs13146272, 1% for rs2227589, and 67% for rs1613662. Conclusions: Three SNPs previously reported to be associated with DVT were found to be associated with PE iso . Because of the high frequencies of two of these polymorphisms in Caucasians, these SNPs are likely make an appreciable contribution to the population attributable risk for PE iso .
Abstract 9155: Intravenous Adenosine Reveals Arrhythmogenic Foci in Patients with Atrial Fibrillation Undergoing Radiofrequency Catheter Ablation
Authors: Ippei Tsuboi, Yasushi Miyauchi, Meiso Hayashi, Kenta Takahashi, Shunsuke Uetake, Hiroshi Hayashi, Ayano Nakatsuji, Hiroshige Murata, Teppei Yamamoto, Tsutomu Horie, Takao Katoh, Kyoichi Mizuno
Journal: Circulation (2011)

Background: Intravenous adenosine often induces atrial fibrillation (AF). However, the role of adenosine in patients with AF undergoing radiofrequency ablation is not determined. We tested the hypothesis that intravenous adenosine reveals culprit site for AF. Method: We included 75 consecutive patients (57 male, 62±10 years) with symptomatic AF undergoing pulmonary vein (PV) isolation for either paroxysmal (N=63) or persistent (N=12) AF in whom PV venography was performed during sinus rhythm. For PV venography, intravenous adenosine triphosphate (ATP) at a dose of 40mg was administered to produce transient asystole. We determined the incidence and origin of ectopic beats emerged after intravenous ATP utilizing simultaneous recording of the left and right superior PVs, left inferior PV, coronary sinus, right atrial free wall, septum and superior vena cava (Figure). For each PV, a straight 10-pole catheter was used to determine the longitudinal activation sequence. Results: Repetitive beats initiating AF (RBAF) or single to triplet of premature beats (SPB) were induced within 1 minute after administration of ATP in 29 patients (39%) and 57 patients (76%), respectively. In 22 patients with RBAF from the PVs, the AF-free rate after 1.3±0.5 sessions of PVI was 86%, whereas that in 7 patients with extra-PV RBAF was 57% (P= 0.15). A total of 111 SPB origin was identified in 57 patients. The origin of 37 SPBs was in the PV, and that of the remaining 74 SPB was outside the PV. The AF-free rate in patients with SPB restricted in the PVs was 88%, and that with extra-PV SPB was 78% (P= 0.52) after PVI. In 9 patients in whom neither RBAF nor SPB was induced by ATP, AF-free rate was 67%. Conclusion: In most patients, RBAF or SPB could be induced by intravenous ATP within 1 minute from the injection, revealing culprit site for AF. Induction of extra-PV RBAF or non-inducibility of either RBAF or SPB correlates with higher incidence of recurrence. However, extra-PV SPB is less significant.
EBF Deficient Hematopoietic Progenitor Cells Potentialy Differentiated into Immature B Cell: EBF1 Dispensable for B Cell Linage Commitment from Pro-B to Pre-B Stage
Authors: Bidisha Chanda, Tomokatsu Ikawa, Kazuki Okuyama, Katsuto Hozumi, Kiyoshi Ando, Arinobu Tojo, Hiroshi Kawamoto, Ai Kotani
Journal: Blood (2014)
Abstract 5939: Blockade of a7-Nicotinic Acetylcholine Receptors Attenuates Cardio-protective Effects of Donepezil in Rats With Extensive Myocardial Infarction
Authors: Meihua Li, Can Zheng, Masashi Inagaki, Kazunori Uemura, Toshiaki Shishido, Tsuyoshi Akiyama
Journal: Circulation (2009)

Introduction: We have reported that donepezil, an acetylcholinesterase inhibitor has improved long-term survival of rats with chronic heart failure (CHF) after extensive myocardial infarction (MI),...
Commentary: Socioeconomic deprivation and health and the ecological fallacy
Authors: K. MacRae
Journal: BMJ (1994)

people of different birth weights differ is unlikely to be adequately represented by socioeconomic status as indicated by occupation.What is clear is that more research is required on the interrelation between early development, adult experiences and behaviour, and later risk of mortality.This research should be carried out in recognition of the fact that, particularly in the presence of crudely measured variables such as occu- pational social class, confounding can produce strong associations which are resistant to simple interpretations and are robust to attempts at statistical "control.
Abstract 419: Human ApoB100/CETP Transgenic Mouse is a Useful Animal Model for Evaluation of HDL-C Elevation and Suppression of Atherosclerosis by Peroxisome Proliferator-Activated Receptor Delta Agonist
Authors: Noriyuki Naya, Keita Fukao, Akemi Nakamura, Kohji Nomura, Seijiro Hara, Kohji Hanasaki
Journal: Circulation (2008)

PPARδ is one of the transcription factors that regulate lipid metabolism. Recently, PPARδ agonists have been reported to induce the elevation of plasma HDL-C levels in obese mice, rhesus monkeys as well as humans, indicating their potentials as a new class of HDL-C raising agent. In addition, anti-atherosclerotic effects of PPARδ agonists were reported in LDLR-KO and ApoE-KO mice. In these mice, however, the lipoprotein profiles are greatly different from those in humans in terms of the deficiency in CETP and ApoB100, and thus the anti-inflammatory effects were regarded as a main anti-atherosclerotic mechanism of PPARδ agonists. It has been reported that human ApoB100/CETP transgenic (hApoB100/CETP-Tg) mice have similar lipoprotein profiles with humans. In this study, we used hApoB100/CETP-Tg mice placed under a western diet for evaluation of GW501516, one of the most potent and selective PPARδ agonists, in order to investigate the linkage between HDL-C elevation and anti-atherosclerotic potency. For evaluation of plasma HDL-C levels, the hApoB100/CETP-Tg mice were orally treated with GW501516 for 1 week and its potency was compared with fenofibrate, a PPARα agonist used in the clinic. For evaluation of the anti-atherosclerotic effect, the mice were orally treated with GW501516 for 18 weeks and atherosclerosis at the aortic valves was determined by cross-sectional lesion analysis. Serum lipoprotein parameters were periodically monitored and lipoprotein profiles were analyzed by FPLC method. Treatment with GW501516 resulted in significant elevation of plasma HDL-C levels with more potency compared to fenofibrate (24% and 15% elevation at 10mg/kg, respectively). Serum apoA-I was also increased by the similar ratio as HDL-C, and the particle size of HDL was not changed, suggesting that the numbers of HDL particle are increased by GW501516. Long term treatment of GW501516 (3 and 10 mg/kg) resulted in dose-dependent suppression of atherosclerosis (42 and 57% inhibition, respectively) with a strong correlation with HDL-C elevation (p&lt;0.001). By using hApoB100/CETP-Tg mice, PPARδ was confirmed as a promising target of anti-atherosclerotic therapy as a new class of HDL-C raising agent.
Abstract 16606: Brugada-Like ECG Changes are Easily Induced With High Precordial Lead Position During Preparticipation ECG Screening in Collegiate Athletes
Authors: Eugene H Chung, Sharon Evans, Edmund Pryski, David Mcneely, Thomas Brickner, Kelly Waicus, Mario Ciocca
Journal: Circulation (2011)

Introduction The use of high precordial leads in the 2nd or 3rd intercostal space is one means of evaluating patients with suspected Brugada syndrome. During our first year of preparticipation ECG ...
Detection of hypermethylation in the CpG Islands of the p16<sup>INK4A </sup>and methyl-guanine methyl-transferase (MGMT) gene promoters in pancreatic adenocarcinoma (PC)
Authors: S. Voyatzi, D. Paikos, I. Boukovinas, A. Kiziridou, P. Stravoravdi, I. Stergiou, T. Toliou
Journal: Journal of Clinical Oncology (2008)

14656 Background: Pancreatic cancer consists of an accumulation of genetic and epigenetic alterations. DNA methylation is an important epigenetic mechanism of transcriptional regulation during tumor development. We examined the methylation status of p16 INK4A and MGMT tumor suppressor genes considered to be inactivated by promoter hypermethylation in several tumours. p16INK4A gene is an important negative regulator of cell proliferation. MGMT gene removes mutagenic adducts from the O6- position of guanine in DNA. Little is known about the exact role of hypermethylation of both genes in PC, as the molecular mechanisms underlying these neoplasms remain poorly understood. Methods: Tumor specimens from 13 PC patients (M/F: 8/5, mean age 67.2 years, range 54 -79) were evaluated for epigenetic alterations of p16INK4A and MGMT genes. Genomic DNA was extracted from formalin-fixed paraffin-embedded tumor tissue. DNA methylation was determined by chemical modification of genomic DNA with sodium bisulfite and subsequent double “hot start” Methylation-Specific PCR (MSP). All double MSPs were performed with controls for unmethylated and methylated alleles followed by detection on agarose gel (electrophoresis). Our results on promoter methylation of both genes were correlated with known clinicopathological parameters. Results: The cell cycle control gene p16INK 4A was methylated in all 13 pts. In contrast, the mismatch repair gene MGMT was methylated in the only patient with poorly differentiated PC. Lymph node status did not correlate with methylation of gene promoters in PC pts. Conclusions: Our findings suggest that p16INK 4A gene is commonly down regulated in PC through a promoter hypermethylation of CpG islands. Further studies of promoter hypermethylation within selected genes may afford an accurate diagnostic molecular marker and predictor of neoplastic behaviour of PC. No significant financial relationships to disclose.
Abstract 15458: Inside-out Access: A New Method of Lead Placement for Patients with Central Venous Occlusions
Authors: Christopher L Allen, Claude S Elayi, Steve Leung, Shamik Aikat, Matt Wiisanen, Gustavo X Morales, Bahram Kakavand, Jignesh Shah, David J Moliterno, John C Gurley
Journal: Circulation (2010)

Objective: We describe a new method of vascular access that allows pre-pectoral placement of conventional pacing and defibrillation leads in patients with complex central venous occlusions that can...
Abnormal growth hormone response to LRH and TRH in adolescent schizophrenic boys
Authors: I Gil-Ad, Z Dickerman, R Weizman, A Weizman, S Tyano, Z Laron
Journal: American Journal of Psychiatry (1981)

The authors studied the effect of LRH and TRH on HGH in 10 adolescent schizophrenic boys and 9 age-matched normal boys. Before antipsychotic treatment, LRH and TRH induced a marked rise in hGH in 8 of 10 patients and 4 of 6 patients, respectively. No effect on HGH was observed in the normal controls. After 3 months of treatment with chlorpromazine, thioridazine, or haloperidol, LRH failed to induce a rise in HGH in 5 of the 6 patients tested, but TRH induced a significant rise in HGH in 3 of 4 patients tested. The authors postulate that these results indicate a dysfunction in the mechanism regulating HGH secretion in schizophrenia.
Abstract 140: High-Fideility Simulation Training Adds No Value to Performance of Resuscitation Skills After 2 Years
Authors: Paul Cash, R Lee Williams, Leonid Bunegin
Journal: Circulation (2010)

This study evaluates the ability of Advanced Cardiac Life Support (ACLS) providers, returning for re-certification after a 2 year hiatus, to resuscitate a High Fidelity Human Simulator (HFHS) in ve...
Swallowed foreign bodies
Authors: B H FEDER, G G MYERS
Journal: BMJ (1975)

Of 98 swallowed foreign bodies demonstrated, 71 with fate definitely known are reported. Seventeen of 20 foreign bodies in the esophagus had esophagoscopic removal. Only two of 51 foreign bodies in the gastrointestinal tract had laparotomy, while 49 were spontaneously passed. Early esophagoscopic removal of foreign bodies lodged in the esophagus and conservative management of foreign bodies which have passed the esophagogastric junction are recommended. Laparotomy is rarely indicated in the management of swallowed foreign bodies, although various observers are not in full agreement as to the circumstances in which "watchful waiting" is advisable nor as to how long it is permissible to wait for spontaneous passage. In 20 of 71 cases of swallowed foreign bodies, the objects were in the esophagus at the time the patient was first examined. Esophagoscopic removal was carried out in 17 cases. In two cases a foreign body was passed per rectum and in one was vomited. Laparotomy for removal was done in only two of the 51 cases in which the foreign body was already in the stomach or bowel at the time of examination, and in one of them the operation probably could have been avoided.
Abstract 10586: Cardiac Adipose Tissue-Derived Stem Cells Exhibit High Differentiation Potential to Cardiovascular Lineage Cells
Authors: Hiroki Nagata, Masaaki Ii, Eiko Kohbayashi, Masaaki Hoshiga, Toshiaki Hanafusa, Michio Asahi
Journal: Circulation (2011)

Background: Adipose-derived stem cells (AdSCs) have recently been shown to differentiate into cardiovascular lineage cells. However, little is known about fat tissue origin-dependent difference of ...
Erythropoietin and Interleukin-3 Activate Tyrosine Phosphorylation of CBL and Association With CRK Adaptor Proteins
Authors: Dwayne L. Barber, Jacqueline M. Mason, Toru Fukazawa, Kris A. Reedquist, Brian J. Druker, Hamid Band, Alan D. D'Andrea
Journal: Blood (1997)
Data collection should be integrated with patient care
Authors: M. Joffe, J. Chapple, R W Beard
Journal: BMJ (1995)

Data collection should be integrated with patient care EDITOR,-The editorial on making routine data adequate to support clinical audit mentions the high quality of data from the St Mary's maternity information system,' citing two recent papers on its validity.23The editorial's authors, however, have missed the point about why this particular system has proved so successful.The basis of the system is that midwives enter the data themselves and then in turn use the system for practical purposes directly associated with patients' care- for example, it generates summaries and discharge letters.The system is thus integrally associated with patients' care and is not a separate task.This experience strongly suggests that abandon- ing the normal practice of relegating the input of data to clerical staff results in better quality of data.This is both because the motivation of health care professionals is higher and, crucially, because of the reinforcing feedback loop involving the use of the information for patients' care.The Department of Health has introduced an information management and technology strategy for the NHS in England, which has many positive features.4Its potential Achilles' heel, however, is precisely this question of the quality of the data.Although it includes a data quality initiative, this is concerned mainly with administrative data (the postcode and codes for the provider, health authority, and general practitioner), the only clinical element being the code for the diagnosis.5We suggest that a great deal could be learnt from the strengths and weaknesses of existing systems, especially successful ones like St Mary's maternity information system, and could then be used in the development of the future information technology for health care.
Abstract 16536: Characterizing the PKA and PKG Phosphoproteomes of the Cardiac Myocyte
Authors: Ronald J Holewinski, Khalid Chakir, David A Kass, Jennifer E Van Eyk
Journal: Circulation (2012)

Protein phosphorylation is an important post translational modification involved in cellular signaling. Disruption of this balance alters cellular phenotype and in some cases can lead to adverse ef...
Abstract 3750: Prevalence of Coronary Artery Disease in New York City Police Officers as Predicted by Coronary Artery Calcium Scoring
Authors: Jia Lin See, Nikolas Wanahita, Nir N Somekh, Stephen E Nelson, Albert Barrette, Kenneth Giedd, Steven R Bergmann
Journal: Circulation (2007)

Background: Recent studies in police officers and firefighters have shown that physically taxing and psychologically stressful occupations may increase death from coronary artery disease (CAD). The aim of this study was to determine if there is an increased prevalence of CAD among members of the New York City Police Department (NYPD). Methods: A total of 2,068 NYPD police officers and detectives underwent electron beam computed tomography for quantitation of coronary artery calcium (CAC) using the Agatston scoring method. The CAC score is known to correlate with the extent and severity of CAD and is predictive of adverse cardiovascular events. The CAC scores were compared with a gender- and age-specific database developed by Hoff et al (also known as the Kondos database). Patients with CAC scores &gt; 400 and those whose scores fall within the upper quartile for gender and age are generally considered to be at increased risk of adverse events. Results: Participants’ mean age was 42 ± 6 years and 86% were male. More than 50% of males &lt; 65 and females &lt; 60 years of age had scores below the 50 th percentile for their age group (Tables ). A subset of 75 individuals (mean age 41 ± 6; 88% male) with known early exposure to the World Trade Center (WTC) collapse were evaluated; they did not have increased CAC scores (data not shown). Conclusion: There is not an increased prevalence of CAD among members of the NYPD compared to the general population as assessed with CAC quantitation. Early exposure to the WTC collapse does not appear to increase the risk of premature CAD at five years.
Abstract 9549: Influence of Physical Activity on Warfarin Dose, Anticoagulation Response and Risk of Hemorrhagic Complications
Authors: Aditi Shendre, T. Mark Beasley, Todd M Brown, Charles E Hill, Donna K Arnett, Nita A Limdi
Journal: Circulation (2013)

Importance: Response to warfarin is influenced by a multitude of patient-specific factors including genetics, clinical comorbidities, and concomitant medications. Objective: To determine the influence of consistent physical activity on stable warfarin dose (mg/day), anticoagulation control (percent-time-in-target-range; PTTR) and risk of major hemorrhage among patients on chronic anticoagulation therapy. Methods: Physical activity was ascertained by self-report at initiation of warfarin therapy (target INR=2-3) in 1276 patients with changes documented at monthly anticoagulation clinic visits. The association of consistent physical activity (maintained over &gt;80% of visits) on warfarin response and anticoagulation control was evaluated using multi-variable linear regression analysis and for major hemorrhage using survival analysis. Results: The number of participants who were consistently physically active (&gt;30 minutes &gt;3 times/week) were 683 (53.7%). Physically active patients required 6.9% higher warfarin doses (p=0.006) and achieved better, although not statistically significant, anticoagulation control (50% vs. 48%; p=0.13) with fewer clinic visits (1.9 vs. 2.5 per patient/ month; p&lt;0.0001) compared to physically inactive patients after accounting for demographic, clinical and genetic factors. There were 137 major hemorrhagic events encountered over 1801 person years of follow-up (incidence 7.6/100pyrs; 95% CI: 6.4 -8.9). The incidence of hemorrhage was lower among physically active patients (5.6/100pyrs; 95% CI: 4.2-7.2) compared to inactive patients (10.3/100 pyrs; 95% CI: 8.2-12.9; p=0.0004). Active patients had a 38% lower risk of hemorrhage (HR: 0.62, 95% CI: 0.42-0.98, p= 0.03) compared to inactive patients after adjusting for bleeding risk (HAS-BLED score), clinical and genetic factors. Conclusion and Relevance: Consistent physical activity is associated with higher warfarin dose requirements but with improved anticoagulation control and lower risk of hemorrhage. In addition to the beneficial effects of physical activity to health and longevity, its influence on drug response needs to be better understood and the mechanisms through which physical activity exerts these effects need to be elucidated.