tbuckley/cabot-search huggingface dataset:
Cell-Free DNA Maps Tissue Injury and Correlates with Disease Severity in Lung Transplant Candidates
Authors: Shanti Balasubramanian, Mary E. Richert, Hyesik Kong, Sheng Fu, Moon Kyoo Jang, Temesgen E. Andargie, Michael B. Keller, Muhtadi Alnababteh, Woojin Park, Zainab Apalara, Jian Sun, Neelam Redekar, Jonathan Orens, Shambhu Aryal, Errol L. Bush, Edward Cantu, Joshua Diamond, Pali Shah, Kai Yu, Steven D. Nathan, Sean Agbor-Enoh
Journal: American Journal of Respiratory and Critical Care Medicine (2023)

Plasma cell-free DNA levels correlate with disease severity in many conditions. Pretransplant cell-free DNA may risk stratify lung transplant candidates for post-transplant complications.
Screening Second Victims for Emotional Distress: Assessment of the Clinimetric Properties of the WITHSTAND-PSY Questionnaire
Authors: Isolde Martina Busch, Maria Angela Mazzi, Loretta Berti, Albert W. Wu, Fiammetta Cosci, Veronica Marinelli, Francesca Moretti, Michela Rimondini
Journal: Psychotherapy and Psychosomatics (2023)

&lt;b&gt;&lt;i&gt;Introduction:&lt;/i&gt;&lt;/b&gt; Adverse events (AEs) are a leading cause of patient morbidity and mortality, greatly impacting healthcare providers’ well-being (second victim (SV) phenomenon). Since it is not accurately captured by existing psychometric instruments, we developed a clinimetric instrument for assessing SVs’ emotional distress before and after an AE. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Content validity and clinical utility of the WITHSTAND-PSY Questionnaire (WS-PSY-Q) were examined using cognitive interviews. Rasch analysis (&lt;i&gt;n&lt;/i&gt; = 284) was applied for clinimetric assessment (i.e., construct, concurrent, and clinical validity, internal consistency), considering two crucial psychological facets of the SV phenomenon (1st: &lt;i&gt;emotional impact of the AE&lt;/i&gt;, 2nd: &lt;i&gt;current emotional state&lt;/i&gt;). &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; The Rasch partial credit model was used. The 1st facet demonstrated overall acceptable clinimetric properties with the subscale anxiety meeting clinimetric threshold values (e.g., all items with ordered thresholds, Loevinger’s coefficient &lt;i&gt;h&lt;/i&gt; ≥ 0.40; Person Separation Reliability Index (PSI) = 0.7). The 2nd facet showed overall better clinimetric properties for both subscales (e.g., &lt;i&gt;h&lt;/i&gt; ≥ 0.40, PSI = 0.82 and 0.79, respectively; receiver operating characteristic area of 0.80 and 0.86, respectively). For both datasets, item fit statistics, except those for item 19, were within the critical range (z-score &amp;lt; ±2.5), and meaningful differential functioning analysis was observed for only 4 (out of 24) items. Local dependency was not observed, except for two item couples in the depression subscales. &lt;b&gt;&lt;i&gt;Conclusions:&lt;/i&gt;&lt;/b&gt; The WS-PSY-Q is the first clinimetric tool assessing SVs’ emotional distress. It should be regarded as part of the armamentarium used by clinicians to assess in-depth healthcare providers’ psychological reactions in the aftermath of an AE to mitigate burnout and allostatic overload.
A conserved transcriptional program for MAIT cells across mammalian evolution
Authors: Hélène Bugaut, Yara El Morr, Martin Mestdagh, Aurélie Darbois, Rafael A. Paiva, Marion Salou, Laetitia Perrin, Mariela Fürstenheim, Anastasia du Halgouet, Linda Bilonda-Mutala, Anne-Laure Le Gac, Manon Arnaud, Ahmed El Marjou, Coralie Guerin, Atitheb Chaiyasitdhi, Julie Piquet, David M. Smadja, Agata Cieslak, Bernhard Ryffel, Valdone Maciulyte, James M.A. Turner, Karine Bernardeau, Xavier Montagutelli, Olivier Lantz, François Legoux
Journal: The Journal of Experimental Medicine (2023)

Mucosal-associated invariant T (MAIT) cells harbor evolutionarily conserved TCRs, suggesting important functions. As human and mouse MAIT functional programs appear distinct, the evolutionarily conserved MAIT functional features remain unidentified. Using species-specific tetramers coupled to single-cell RNA sequencing, we characterized MAIT cell development in six species spanning 110 million years of evolution. Cross-species analyses revealed conserved transcriptional events underlying MAIT cell maturation, marked by ZBTB16 induction in all species. MAIT cells in human, sheep, cattle, and opossum acquired a shared type-1/17 transcriptional program, reflecting ancestral features. This program was also acquired by human iNKT cells, indicating common differentiation for innate-like T cells. Distinct type-1 and type-17 MAIT subsets developed in rodents, including pet mice and genetically diverse mouse strains. However, MAIT cells further matured in mouse intestines to acquire a remarkably conserved program characterized by concomitant expression of type-1, type-17, cytotoxicity, and tissue-repair genes. Altogether, the study provides a unifying view of the transcriptional features of innate-like T cells across evolution.
CD163+ macrophages monitor enhanced permeability at the blood–dorsal root ganglion barrier
Authors: Harald Lund, Matthew A. Hunt, Zerina Kurtović, Katalin Sandor, Paul B. Kägy, Noah Fereydouni, Anais Julien, Christian Göritz, Elisa Vazquez-Liebanas, Maarja Andaloussi Mäe, Alexandra Jurczak, Jinming Han, Keying Zhu, Robert A. Harris, Jon Lampa, Jonas Heilskov Graversen, Anders Etzerodt, Lisbet Haglund, Tony L. Yaksh, Camilla I. Svensson
Journal: The Journal of Experimental Medicine (2023)

In dorsal root ganglia (DRG), macrophages reside close to sensory neurons and have largely been explored in the context of pain, nerve injury, and repair. However, we discovered that most DRG macrophages interact with and monitor the vasculature by sampling macromolecules from the blood. Characterization of the DRG vasculature revealed a specialized endothelial bed that transformed in molecular, structural, and permeability properties along the arteriovenous axis and was covered by macrophage-interacting pericytes and fibroblasts. Macrophage phagocytosis spatially aligned with peak endothelial permeability, a process regulated by enhanced caveolar transcytosis in endothelial cells. Profiling the DRG immune landscape revealed two subsets of perivascular macrophages with distinct transcriptome, turnover, and function. CD163+ macrophages self-maintained locally, specifically participated in vasculature monitoring, displayed distinct responses during peripheral inflammation, and were conserved in mouse and man. Our work provides a molecular explanation for the permeability of the blood-DRG barrier and identifies an unappreciated role of macrophages as integral components of the DRG-neurovascular unit.
Extracellular Proteins Isolated from <i>L. acidophilus</i> as an Osteomicrobiological Therapeutic Agent to Reduce Pathogenic Biofilm Formation, Regulate Chronic Inflammation, and Augment Bone Formation In Vitro
Authors: Abinaya Sindu Pugazhendhi, Anouska Seal, Megan Hughes, Udit Kumar, Elayaraja Kolanthai, Fei Wei, Jonathan D. Schwartzman, Melanie J. Coathup
Journal: Advanced Healthcare Materials (2023)

Periprosthetic joint infection (PJI) is a challenging complication that can occur following joint replacement surgery. Efficacious strategies to prevent and treat PJI and its recurrence remain elusive. Commensal bacteria within the gut convey beneficial effects through a defense strategy named "colonization resistance" thereby preventing pathogenic infection along the intestinal surface. This blueprint may be applicable to PJI. The aim is to investigate Lactobacillus acidophilus spp. and their isolated extracellular-derived proteins (LaEPs) on PJI-relevant Staphylococcus aureus, methicillin-resistant S. aureus, and Escherichia coli planktonic growth and biofilm formation in vitro. The effect of LaEPs on cultured macrophages and osteogenic, and adipogenic human bone marrow-derived mesenchymal stem cell differentiation is analyzed. Data show electrostatically-induced probiotic-pathogen species co-aggregation and pathogenic growth inhibition together with LaEP-induced biofilm prevention. LaEPs prime macrophages for enhanced microbial phagocytosis via cathepsin K, reduce lipopolysaccharide-induced DNA damage and receptor activator nuclear factor-kappa B ligand expression, and promote a reparative M2 macrophage morphology under chronic inflammatory conditions. LaEPs also significantly augment bone deposition while abating adipogenesis thus holding promise as a potential multimodal therapeutic strategy. Proteomic analyses highlight high abundance of lysyl endopeptidase, and urocanate reductase. Further, in vivo analyses are warranted to elucidate their role in the prevention and treatment of PJIs.
Covalent Coupling Assisted Hydrophilic Perovskite Spheres for Ratiometric Fluorescent Visual Multichannel Immunoassay
Authors: Yaqin Han, Jia An, Mengdi Yuan, Junan Fang, Jiajing Zhang, Lanju Liang, Yufei Liu
Journal: Advanced Healthcare Materials (2023)

Abstract Quantitative fluorescence immunoassay is essential for the construction of biosensing mechanisms and the quantification of trace markers. But the interference problems caused by low fluorescence efficiency and broad fluorescence spectrum of fluorescent probes have hindered the continued development of ratiometric fluorescence sensing in biosensing. Perovskite materials, with ultra‐high color purity (FWHM &lt; 30 nm) and photoluminescence quantum yield (PLQY) (close to 100%), are expected to be next‐generation fluorescent probes. However, poor water stability and biocompatibility are still non‐negligible in biosensor applications. In this work, hyperstatic perovskite fluorescent microspheres prepared by swelling‐shrinking method can be used as ratiometric fluorescence signals and biological immunoassay platforms. Meanwhile, inspired by p‐aminophenol (AP) controlled synthesis and the catalytic reaction of 4‐aminophenol phosphate (APP) triggered by alkaline phosphatase (ALP), a strategy to prepare fluorescent nanoparticles as fluorescence signals for ALP detection is proposed. Most importantly, it is proposed for the first time to combine this enzymatic fluorescence with perovskite materials using covalent linkage to create a novel cascade immunoassay and use it for quantitative and visualization determination of hepatitis B surface antigen (HBsAg) for application verification. These results indicate the biosensing potential of perovskite materials and provide a pathway for high sensitivity enzyme detection and enzyme triggered immune detection.
Circulating metabolic markers after surgery identify patients at risk for severe postoperative complications: a prospective cohort study in colorectal cancer
Authors: Blanca Montcusí, Francisco Madrid-Gambin, Óscar J Pozo, Santiago Marco, Silvia Marin, Xavier Mayol, Marta Pascual, Sandra Alonso, Silvia Salvans, Marta Jiménez-Toscano, Marta Cascante, Miguel Pera
Journal: International Journal of Surgery (2023)

Background: Early detection of postoperative complications after colorectal cancer (CRC) surgery is associated with improved outcomes. The aim was to investigate early metabolomics signatures capable to detect patients at risk for severe postoperative complications after CRC surgery. Materials and methods: Prospective cohort study of patients undergoing CRC surgery from 2015 to 2018. Plasma samples were collected before and after surgery, and analyzed by mass spectrometry obtaining 188 metabolites and 21 ratios. Postoperative complications were registered with Clavien–Dindo Classification and Comprehensive Complication Index. Results: One hundred forty-six patients were included. Surgery substantially modified metabolome and metabolic changes after surgery were quantitatively associated with the severity of postoperative complications. The strongest positive relationship with both Clavien–Dindo and Comprehensive Complication Index (β=4.09 and 63.05, P &lt;0.001) corresponded to kynurenine/tryptophan, against an inverse relationship with lysophosphatidylcholines (LPCs) and phosphatidylcholines (PCs). Patients with LPC18:2/PCa36:2 below the cut-off 0.084 µM/µM resulted in a sevenfold higher risk of major complications (OR=7.38, 95% CI: 2.82–21.25, P &lt;0.001), while kynurenine/tryptophan above 0.067 µM/µM a ninefold (OR=9.35, 95% CI: 3.03–32.66, P &lt;0.001). Hexadecanoylcarnitine below 0.093 µM displayed a 12-fold higher risk of anastomotic leakage-related complications (OR=11.99, 95% CI: 2.62–80.79, P =0.004). Conclusion: Surgery-induced phospholipids and amino acid dysregulation is associated with the severity of postoperative complications after CRC surgery, including anastomotic leakage-related outcomes. The authors provide quantitative insight on metabolic markers, measuring vulnerability to postoperative morbidity that might help guide early decision-making and improve surgical outcomes.
Impact of ketamine on postoperative delirium: insights from trial sequential analysis
Authors: Kuo-Chuan Hung, I-Wen Chen, Ping-Hsin Liu
Journal: International Journal of Surgery (2023)

aDepartment of Anesthesiology, Chi Mei Medical Center, Tainan city, Taiwan bDepartment of Anesthesiology, Chi Mei Medical Center, Liouying, Tainan city, Taiwan cDepartment of Anesthesiology, E-Da Dachang Hospital, I-Shou University, Kaohsiung City, Taiwan Short title: ketamine for postoperative delirium Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article. Published online ■ ■ *Corresponding author. Address: Department of Anesthesiology, E-Da Dachang Hospital, I-Shou University, No. 305, Dachang 1st Road, Sanmin District, Kaohsiung City, Taiwan, Tel.: +886-7-6150011 ext 1007; Fax: +886-7-6150915. E-mail address: [email protected] (P-H. Liu). This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/
Prescribed Performance Control of a Human-Following Surveillance Robot with Incomplete Observation
Authors: Meiping Zhang, Zixin Chi, Zhaohui Yang, Samer Mohammed, Jian Huang
Journal: Cyborg and Bionic Systems (2023)

For people with lower limb muscle weakness, effective and timely rehabilitation intervention is essential for assisting in daily walking and facilitating recovery. Numerous studies have been conducted on rehabilitation robots; however, some critical issues in the field of human-following remain unaddressed. These include potential challenges related to the loss of sensory signals for intention recognition and the complexities associated with maintaining the relative pose of robots during the following process. A human-following surveillance robot is introduced as the basis of the research. To address potential interruptions in motion signals, such as data transmission blockages or body occlusion, we propose a human walking intention estimation algorithm based on set-membership filtering with incomplete observation. To ensure uninterrupted user walking and maintain an effective aid and detection range, we propose a human-following control algorithm based on prescribed performance. The experiment verifies the effectiveness of the proposed methods. The proposed intention estimation algorithm achieves continuous and accurate intention recognition under incomplete observation. The control algorithm presented in this paper achieves constrained robot following with respect to the relative pose.
BridgePRS leverages shared genetic effects across ancestries to increase polygenic risk score portability
Authors: Clive J. Hoggart, Shing Wan Choi, Judit García-González, Tade Souaiaia, Michael Preuss, Paul F. O’Reilly
Journal: Nature Genetics (2023)

Here we present BridgePRS, a novel Bayesian polygenic risk score (PRS) method that leverages shared genetic effects across ancestries to increase PRS portability. We evaluate BridgePRS via simulations and real UK Biobank data across 19 traits in individuals of African, South Asian and East Asian ancestry, using both UK Biobank and Biobank Japan genome-wide association study summary statistics; out-of-cohort validation is performed in the Mount Sinai (New York) BioMe biobank. BridgePRS is compared with the leading alternative, PRS-CSx, and two other PRS methods. Simulations suggest that the performance of BridgePRS relative to PRS-CSx increases as uncertainty increases: with lower trait heritability, higher polygenicity and greater between-population genetic diversity; and when causal variants are not present in the data. In real data, BridgePRS has a 61% larger average R
Oral nitrate supplementation improves cardiovascular risk markers in COPD: ON-BC, a randomised controlled trial
Authors: Ali M. Alasmari, Abdullah S. Alsulayyim, Saeed M. Alghamdi, Keir E.J. Philip, Sara C. Buttery, Winston A.S. Banya, Michael I. Polkey, Paul C. Armstrong, Matthew J. Rickman, Timothy D. Warner, Jane A. Mitchell, Nicholas S. Hopkinson
Journal: European Respiratory Journal (2023)

Short-term studies suggest that dietary nitrate (NO
Recent Developments in Layer‐by‐Layer Assembly for Drug Delivery and Tissue Engineering Applications
Authors: João Borges, Jinfeng Zeng, Xi Qiu Liu, Hao Chang, Claire Monge, Charlotte Garot, Ke‐feng Ren, Paul Machillot, Nihal E. Vrana, Philippe Lavalle, Takami Akagi, Michiya Matsusaki, Jian Ji, Mitsuru Akashi, João F. Mano, Varvara Gribova, Catherine Picart
Journal: Advanced Healthcare Materials (2023)

Surfaces with biological functionalities are of great interest for biomaterials, tissue engineering, biophysics, and for controlling biological processes. The layer-by-layer (LbL) assembly is a highly versatile methodology introduced 30 years ago, which consists of assembling complementary polyelectrolytes or biomolecules in a stepwise manner to form thin self-assembled films. In view of its simplicity, compatibility with biological molecules, and adaptability to any kind of supporting material carrier, this technology has undergone major developments over the past decades. Specific applications have emerged in different biomedical fields owing to the possibility to load or immobilize biomolecules with preserved bioactivity, to use an extremely broad range of biomolecules and supporting carriers, and to modify the film's mechanical properties via crosslinking. In this review, the focus is on the recent developments regarding LbL films formed as 2D or 3D objects for applications in drug delivery and tissue engineering. Possible applications in the fields of vaccinology, 3D biomimetic tissue models, as well as bone and cardiovascular tissue engineering are highlighted. In addition, the most recent technological developments in the field of film construction, such as high-content liquid handling or machine learning, which are expected to open new perspectives in the future developments of LbL, are presented.
Incidence of Uveitis in Patients With Axial Spondylarthritis Treated With Biologics or Targeted Synthetics: A Systematic Review and Network <scp>Meta‐Analysis</scp>
Authors: Katie Bechman, Zijing Yang, Maryam Adas, Deepak Nagra, Ali S. Uğuzlar, Mark D. Russell, Nicky Wilson, Sophia Steer, Sam Norton, James Galloway
Journal: Arthritis & Rheumatology (2023)

Objective Anterior uveitis is a common extra‐articular manifestation of axial spondyloarthritis (AxSpA). We set to evaluate the risk of anterior uveitis (AU) with biologics and synthetic disease‐modifying drugs in AxSpA. Methods We conducted a systematic review and meta‐analysis to identify phase II/III double‐blinded randomized controlled trials of anti–tumor necrosis factor (TNF) monoclonal antibodies (mAb), anti–interleukin‐17 (anti–IL‐17), and Janus kinase inhibitors (JAKi) in AxSpA. Patient‐exposure years (PEY) were calculated using the per‐protocol approach. Incidence rate (IR) of AU/100 person‐years were calculated by treatment group using the random effects approach. Network meta‐analysis (NMA) was used to estimate risk of AU in treatment groups, expressed as IR ratios (IRRs). Bias was assessed using the Cochrane Risk of Bias‐2 tool. Results Forty‐four trials were included: 17 anti‐TNF mAb (1,004 PEY), 9 etanercept (180 PEY), 13 anti–IL‐17 (1,834 PEY), and 6 JAKi (331 PEY). The IR of AU were as follows for anti‐TNF mAb: 4.1, 95% confidence interval (CI) 0–8.5; etanercept: 5.4, 95% CI 0–16.0; anti–IL‐17: 2.8, 95% CI 1.6–4.1; JAKi: 1.5, 95% CI 0.0–3.0; and placebo: 10.8, 95% CI 7.4–14.1. In NMA, IRRs of treatments compared with placebo were as follows for anti‐TNF mAb: 0.32, 95% CI 0.10–1.04; etanercept 0.42, 95% CI 0.08–2.38; anti–IL‐17: 0.43, 95% CI 0.19–0.98; and JAKi: 0.32, 95% CI 0.06–1.67. Comparisons between anti‐TNF mAb, anti–IL‐17, and JAKi did not demonstrate any significant difference in AU risk. Using the surface under the cumulative ranking curve approach to rank AU risk, anti‐TNF mAbs were associated with the lowest risk followed by JAKi, anti–IL‐17, and etanercept. All treatments were ranked superior to placebo. Conclusion Anti‐TNF mAbs, JAKi, and anti–IL‐17 appear protective against AU events in individuals with AxSpA, with no significant differences in risk of AU between treatments.
Bone marrow B cell developmental aberrations and systemic lupus erythematosus: comment on the article by Dong et al
Authors: Ranjeet Singh Mahla
Journal: Arthritis & Rheumatology (2023)

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Aggregation‐Induced Emission Photosensitizer‐Engineered Anticancer Nanomedicine for Synergistic Chemo/Chemodynamic/Photodynamic Therapy
Authors: Bentong Yu, Mingshan Liu, Lei Jiang, Chuan Xu, Huoli Hu, Tong Huang, Dunwu Xu, Ning Wang, Qianying Li, Ben Zhong Tang, Xiaolin Huang, Wan Zhang
Journal: Advanced Healthcare Materials (2023)

Photodynamic therapy (PDT) with aggregation-induced emission (AIE) photosensitizers (PSs) is a promising therapeutic strategy to achieve better anticancer results. However, eradicating solid tumors completely by PDT alone can be difficult owing to the inherent drawbacks of this treatment, and the combination of PDT with other therapeutic modalities provides opportunities to achieve cooperative enhancement interactions among various treatments. Herein, this work presents the construction of a biocompatible nanocomposite, namely CaO
Dual‐Functional Laser‐Guided Magnetic Nanorobot Collectives against Gravity for On‐Demand Thermo‐Chemotherapy of Peritoneal Metastasis
Authors: Longchen Wang, Weijuan Zou, Jian Shen, Shihao Yang, Jingjing Wu, Tao Ying, Xiaojun Cai, Li Zhang, Jianrong Wu, Yuanyi Zheng
Journal: Advanced Healthcare Materials (2023)

Abstract Combining hyperthermic intraperitoneal chemotherapy with cytoreductive surgery is the main treatment modality for peritoneal metastatic (PM) carcinoma despite the off‐target effects of chemotherapy drugs and the ineluctable side effects of total abdominal heating. Herein, a laser‐integrated magnetic actuation system that actively delivers doxorubicin (DOX)‐grafted magnetic nanorobot collectives to the tumor site in model mice for local hyperthermia and chemotherapy is reported. With intraluminal movements controlled by a torque‐force hybrid magnetic field, these magnetic nanorobots gather at a fixed point coinciding with the position of the localization laser, moving upward against gravity over a long distance and targeting tumor sites under ultrasound imaging guidance. Because aggregation enhances the photothermal effect, controlled local DOX release is achieved under near‐infrared laser irradiation. The targeted on‐demand photothermal therapy of multiple PM carcinomas while minimizing off‐target tissue damage is demonstrated. Additionally, a localization/treatment dual‐functional laser‐integrated magnetic actuation system is developed and validated in vivo, offering a potentially clinically feasible drug delivery strategy for targeting PM and other intraluminal tumors.