tbuckley/cabot-search huggingface dataset:
Optimization and validation of a virus‐like particle pseudotyped virus neutralization assay for SARS‐CoV‐2
Authors: Shuo Liu, Li Zhang, Wangjun Fu, Ziteng Liang, Yuanling Yu, Tao Li, Jincheng Tong, Fan Liu, Jianhui Nie, Qiong Lu, Shuaiyao Lu, Weijin Huang, Youchun Wang
Journal: MedComm (2024)

Abstract Spike‐protein‐based pseudotyped viruses were used to evaluate vaccines during the COVID‐19 pandemic. However, they cannot be used to evaluate the envelope (E), membrane (M), and nucleocapsid (N) proteins. The first generation of virus‐like particle (VLP) pseudotyped viruses contains these four structural proteins, but their titers for wild‐type severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) are relatively low, even lower for the omicron variant, rendering them unsuitable for neutralizing antibody detection. By optimizing the spike glycoprotein signal peptide, substituting the complexed M and E proteins with SARS‐COV‐1, optimizing the N protein with specific mutations (P199L, S202R, and R203M), and truncating the packaging signal, PS9, we increased the titer of the wild‐type VLP pseudotyped virus over 100‐fold, and successfully packaged the omicron VLP pseudotyped virus. The SARS‐CoV‐2 VLP pseudotyped viruses maintained stable titers, even through 10 freeze–thaw cycles. The key neutralization assay parameters were optimized, including cell type, cell number, and viral inoculum. The assay demonstrated minimal variation in both intra‐ and interassay results, at 11.5% and 11.1%, respectively. The correlation between the VLP pseudotyped virus and the authentic virus was strong ( r = 0.9). Suitable for high‐throughput detection of various mutant strains in clinical serum. In summary, we have developed a reliable neutralization assay for SARS‐CoV‐2 based on VLP pseudotyped virus.
LABS: linear amplification-based bisulfite sequencing for ultrasensitive cancer detection from cell-free DNA
Authors: Xiao-Long Cui, Ji Nie, Houxiang Zhu, Krissana Kowitwanich, Alana V. Beadell, Diana C. West-Szymanski, Zhou Zhang, Urszula Dougherty, Akushika Kwesi, Zifeng Deng, Yan Li, Danqing Meng, Kevin Roggin, Teresa Barry, Ryan Owyang, Ben Fefferman, Chang Zeng, Lu Gao, Carolyn W. T. Zhao, Yuri Malina, Jiangbo Wei, Melanie Weigert, Wenjun Kang, Ajay Goel, Brian C.-H. Chiu, Marc Bissonnette, Wei Zhang, Mengjie Chen, Chuan He
Journal: Genome biology (2024)

Methylation-based liquid biopsies show promises in detecting cancer using circulating cell-free DNA; however, current limitations impede clinical application. Most assays necessitate substantial DNA inputs, posing challenges. Additionally, underrepresented tumor DNA fragments may go undetected during exponential amplification steps of traditional sequencing methods. Here, we report linear amplification-based bisulfite sequencing (LABS), enabling linear amplification of bisulfite-treated DNA fragments in a genome-wide, unbiased fashion, detecting cancer abnormalities with sub-nanogram inputs. Applying LABS to 100 patient samples revealed cancer-specific patterns, copy number alterations, and enhanced cancer detection accuracy by identifying tissue-of-origin and immune cell composition.
Skin repair and infection control in diabetic, obese mice using bioactive laser-activated sealants
Authors: Shubham Pallod, Rodrigo Aguilera Olvera, Deepanjan Ghosh, Lama Rai, Souzan Brimo, Weston DeCambra, Harsh Girish Sant, Eron Ristich, Vanshika Singh, Muhammad Raisul Abedin, Nicolas Chang, Jeffery L. Yarger, Jung Keun Lee, Jacquelyn Kilbourne, Jordan R. Yaron, Shelley E. Haydel, Kaushal Rege
Journal: Biomaterials (2024)
Reduced atopic march risk in pediatric atopic dermatitis patients prescribed dupilumab versus conventional immunomodulatory therapy: A population-based cohort study
Authors: Teng-Li Lin, Yi-Hsuan Fan, Kuo-Sheng Fan, Chao-Kuei Juan, Yi-Ju Chen, Chun-Ying Wu
Journal: Journal of the American Academy of Dermatology (2024)
Re: Identification of the Optimal Candidates for Nodal Staging with Extended Pelvic Lymph Node Dissection Among Prostate Cancer Patients Who Underwent Preoperative Prostate-specific Membrane Antigen Positron Emission Tomography. External Validation of the Memorial Sloan Kettering Cancer Center and Briganti Nomograms and Development of a Novel Tool
Authors: Petr Macek, Lara Rodriguez-Sanchez, Xavier Cathelineau
Journal: European Urology (2024)
Early menopause and hormone therapy as determinants for lung health outcomes: a secondary analysis using the PLCO trial
Authors: Xiaochun Gai, Yue Feng, Tessa M Flores, Huining Kang, Hui Yu, Kimberly K Leslie, Yiliang Zhu, Jennifer A Doherty, Yan Guo, Steven A Belinsky, Linda S Cook, Shuguang Leng
Journal: Thorax (2024)

Early natural menopause (early-M; <45 years of age) increases the risk of lung morbidities and mortalities in smokers. However, it is largely unknown whether early-M due to surgery demonstrates similar effects and whether menopausal hormone therapy (MHT) is protective against lung diseases.
Stigma in functional neurological disorder (FND) – A systematic review
Authors: Caoimhe McLoughlin, Laura McWhirter, Katerina Pisegna, Marina A.J. Tijssen, Lineke M. Tak, Alan Carson, Jon Stone
Journal: Clinical Psychology Review (2024)

The purpose of this systematic review was to evaluate stigma and Functional Neurological Disorder (FND) regarding: 1) prevalence and associated factors, 2) the nature and context of stigma in FND, and 3) stigma-reduction interventions.
Clinical characteristics associated with joint pain in hidradenitis suppurativa
Authors: Weiying Lu, Winston W Liu, Negar Foolad, Jeffery T Kwock, Tarannum Jaleel
Journal: British Journal of Dermatology (2024)

Although patients with hidradenitis suppurativa (HS) report comorbid arthropathy, clinical characteristics associated with joint pain surrounding HS flares still remain obscure. We found that about one in five patients in our HS cohort reported peri-flare joint pain during their initial visit and that peri-flare joint pain was significantly associated with pain medication prescription (aOR 2.15, P = 0.02) and diagnosis of inflammatory arthritis (aOR 4.14, P &amp;lt; 0.001). Given the debilitating nature of HS flares, the addition of joint pain can further limit daily activities, underscoring the importance of a comprehensive approach to the management of patients with HS.
Real-world experience of bimekizumab for adult patients with plaque psoriasis: A 52-week multicenter retrospective study
Authors: Siddhartha Sood, Alexander Rimke, Brian D. Rankin, Abrahim Abduelmula, Jorge R. Georgakopoulos, Khalad Maliyar, Ahmed Bagit, Fernejoy Leung, Lauren A. Stark, Alim R. Devani, Ronald Vender, Jensen Yeung, Vimal H. Prajapati
Journal: Journal of the American Academy of Dermatology (2024)
Surface Chemistry Engineered Selenium Nanoparticles as Bactericidal and Immuno-modulating Dual-functional Agents for Combating Methicillin-resistant Staphylococcus aureus Infection
Authors: Qingyue Bu, Dan Jiang, Yangyang Yu, Yunqing Deng, Tianfeng Chen, Ligeng Xu
Journal: Drug Resistance Updates (2024)
Previous radiotherapy increases the efficacy of cemiplimab in the treatment of locally advanced and metastatic cutaneous squamous cell carcinoma: A retrospective analysis
Authors: Valerio Nardone, Stefania Napolitano, Federico Gagliardi, Alfonso Esposito, Francesco Caraglia, Giulia Briatico, Camila Scharf, Andrea Ronchi, Ida D'Onofrio, Emma D'Ippolito, Anna Russo, Maria Paola Belfiore, Renato Franco, Giuseppe Argenziano, Fortunato Ciardiello, Alfonso Reginelli, Salvatore Cappabianca, Teresa Troiani
Journal: Journal of the American Academy of Dermatology (2024)

Cemiplimab, a programmed cell death-1 inhibitor approved in 2018 for patients with locally advanced or metastatic cutaneous squamous cell carcinoma (cSCC) who are ineligible for curative therapies, lacks clarity regarding the optimal patient selection despite its known efficacy.
A three-dimensional genomics view for speciation research
Authors: Ashwini V. Mohan, Paula Escuer, Camille Cornet, Kay Lucek
Journal: Trends in Genetics (2024)

Genomic information is folded in a three-dimensional (3D) structure, a rarely explored evolutionary driver of speciation. Technological advances now enable the study of 3D genome structures (3DGSs) across the Tree of Life. At the onset of 3D speciation genomics, we discuss the putative roles of 3DGSs in speciation.
Early adulthood BMI and cardiovascular disease: a prospective cohort study from the China Kadoorie Biobank
Authors: Yuanyuan Chen, Wei Yu, Jun Lv, Dianjianyi Sun, Pei Pei, Huaidong Du, Ling Yang, Yiping Chen, Huanxu Zhang, Junshi Chen, Zhengming Chen, Liming Li, Canqing Yu
Journal: The Lancet Public Health (2024)
Improving hepatocellular carcinoma surveillance in the United Kingdom: challenges and solutions
Authors: Maria Qurashi, Rohini Sharma
Journal: The Lancet Regional Health - Europe (2024)

Hepatocellular carcinoma (HCC) prognosis remains dismal due to late-stage diagnosis; surveillance has been demonstrated to increase early diagnosis rates and receipt of curative treatment. Acknowledging limitations in the evidence base for HCC surveillance, international professional bodies reiterate the recommendation for biannual HCC surveillance and NHS England supports measures aimed to increase surveillance uptake. The current ad hoc provision of HCC surveillance is prone to failures, evident by low surveillance uptake and high numbers of patients being diagnosed outside of surveillance. We discuss challenges related to HCC surveillance in the UK and potential solutions to addressing them. We highlight the requirements of a consistent and effective national surveillance process, and suggest pathways on how this can be achieved.
Effectiveness of telehealth versus in-person care during the COVID-19 pandemic: a systematic review
Authors: Elham Hatef, Renee F. Wilson, Allen Zhang, Susan M. Hannum, Hadi Kharrazi, Stacey A. Davis, Iman Foroughmand, Jonathan P. Weiner, Karen A. Robinson
Journal: npj Digital Medicine (2024)

Abstract In this systematic review, we compared the effectiveness of telehealth with in-person care during the pandemic using PubMed, CINAHL, PsycINFO, and the Cochrane Central Register of Controlled Trials from March 2020 to April 2023. We included English-language, U.S.-healthcare relevant studies comparing telehealth with in-person care conducted after the onset of the pandemic. Two reviewers independently screened search results, serially extracted data, and independently assessed the risk of bias and strength of evidence. We identified 77 studies, the majority of which (47, 61%) were judged to have a serious or high risk of bias. Differences, if any, in healthcare utilization and clinical outcomes between in-person and telehealth care were generally small and/or not clinically meaningful and varied across the type of outcome and clinical area. For process outcomes, there was a mostly lower rate of missed visits and changes in therapy/medication and higher rates of therapy/medication adherence among patients receiving an initial telehealth visit compared with those receiving in-person care. However, the rates of up-to-date labs/paraclinical assessment were also lower among patients receiving an initial telehealth visit compared with those receiving in-person care. Most studies lacked a standardized approach to assessing outcomes. While we refrain from making an overall conclusion about the performance of telehealth versus in-person visits the use of telehealth is comparable to in-person care across a variety of outcomes and clinical areas. As we transition through the COVID-19 era, models for integrating telehealth with traditional care become increasingly important, and ongoing evaluations of telehealth will be particularly valuable.
Engineered low-pathogenic Helicobacter pylori as orally tumor immunomodulators for the stimulation of systemic immune response
Authors: Zishan Zeng, Yue Sun, Jingwen Jiang, Xiaoyu Xu, Huanxin Lin, Wanzhen Li, Dong Zheng, Yanjuan Huang, Chunshun Zhao
Journal: Biomaterials (2024)
The impact of vulval lichen sclerosus in young people: a call for better awareness, individualised maintenance plans and revised guidelines
Authors: Gayle Fischer
Journal: British Journal of Dermatology (2024)

Journal Article Accepted manuscript The impact of vulval lichen sclerosus in young people: a call for better awareness, individualised maintenance plans and revised guidelines Get access Gayle Fischer Gayle Fischer Department of Dermatology, The Royal North Shore Hospital, St Leonards, NSW, AustraliaThe Northern Clinical School of the Faculty of Medicine and Health, The University of Sydney, Sydney, Australia Email:gayle.fischer@sydney.edu.au Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, ljae254, https://doi.org/10.1093/bjd/ljae254 Published: 15 June 2024 Article history Received: 10 June 2024 Accepted: 14 June 2024 Published: 15 June 2024
Cytokeratin 17 expression is commonly observed in keratinocytic skin tumours and controls tissue homeostasis impacting human papillomavirus protein expression
Authors: Daniel Hasche, Martin Hufbauer, Ilona Braspenning-Wesch, Sonja Stephan, Steffi Silling, Gabriele Schmidt, Stephan Krieg, Alexander Kreuter, Baki Akgül
Journal: British Journal of Dermatology (2024)

Abstract Background The structured expression of several keratins in the skin is associated with differentiation status of the epidermal layers, whereas other keratins are upregulated only during wound healing, in skin disorders and in cancers. One of these stress keratins, K17, is correlated with poor prognosis in various cancer types and its loss has been shown to decelerate tumour growth. K17 expression can also be detected in cutaneous squamous cell carcinomas, where ultraviolet irradiation and infection with cutaneous human papillomaviruses are important cofactors. It was previously reported that K17 is upregulated in papillomavirus (PV)-induced benign skin lesions in mice and induces an immunological status that is beneficial for tumour growth. Objectives In order to investigate whether K17 upregulation is induced by PVs, we analysed K17 levels in skin tumour specimens of different animal models and humans. Methods Various immunofluorescence stainings were performed to identify K17 expression as well as levels of E-cadherin, vimentin and CD271. Tissues were further analysed by polymerase chain reaction (PCR), quantitative (q)PCR and enzyme-linked immunosorbent assay to control for PV activity. K17 knockdown cells were generated and effects on viral life cycle were investigated by infection assays, qPCR and Western blotting. Results We showed that K17 is commonly expressed in skin tumours and that its presence is not directly linked to viral oncoprotein expression. Rather, K17 expression seems to be a marker of epithelial differentiation and its absence in tumour tissue is associated with an epithelial-to-mesenchymal transition. We further demonstrated that the absence of K17 in skin tumours increases markers of cancer stem-like cells and negatively affects viral protein synthesis. Conclusions Collectively, our data indicate that K17 expression is a common feature in skin tumorigenesis. While K17 is not primarily targeted by PV oncoproteins, our in vivo and in vitro data suggest that it is an important regulator of epithelial differentiation and thus may play a role in controlling viral protein synthesis.
Investigating corrected QT prolongation with hydroxychloroquine use among patients with cutaneous sarcoidosis: A multicenter retrospective study
Authors: Chinemelum E. Obijiofor, Michelle Sikora, Lynn Liu, Marleigh J. Stern, Kerry M. Hena, Daniel R. Mazori, Steven Friedman, Soutrik Mandal, Avrom S. Caplan
Journal: Journal of the American Academy of Dermatology (2024)
Spider minor ampullate silk protein nanoparticles: an effective protein delivery system capable of enhancing systemic immune responses
Authors: Hairui Yu, Gefei Chen, Linchao Li, Guoqiang Wei, Yanan Li, Sidong Xiong, Xingmei Qi
Journal: MedComm (2024)

Abstract Spider silk proteins (spidroins) are particularly attractive due to their excellent biocompatibility. Spider can produce up to seven different types of spidroins, each with unique properties and functions. Spider minor ampullate silk protein (MiSp) might be particularly interesting for biomedical applications, as the constituent silk is mechanically strong and does not super‐contract in water, attributed to its amino acid composition. In this study, we evaluate the potential of recombinant nanoparticles derived from Araneus ventricosus MiSp as a protein delivery carrier. The MiSp‐based nanoparticles were able to serve as an effective delivery system, achieving nearly 100% efficiency in loading the model protein lysozyme, and displayed a sustained release profile at physiological pH. These nanoparticles could significantly improve the delivery efficacy of the model proteins through different administration routes. Furthermore, nanoparticles loaded with model protein antigen lysozyme after subcutaneous or intramuscular administration could enhance antigen‐specific immune responses in mouse models, through a mechanism involving antigen‐depot effects at the injection site, long‐term antigen persistence, and efficient uptake by dendritic cells as well as internalization by lymph nodes. These findings highlight the transnational potential of MiSp‐based nanoparticle system for protein drug and vaccine delivery.
Natural killer cells in cancer immunotherapy
Authors: DanRu Wang, LingYun Dou, LiHao Sui, Yiquan Xue, Sheng Xu
Journal: MedComm (2024)

Natural killer (NK) cells, as innate lymphocytes, possess cytotoxic capabilities and engage target cells through a repertoire of activating and inhibitory receptors. Particularly, natural killer group 2, member D (NKG2D) receptor on NK cells recognizes stress-induced ligands-the MHC class I chain-related molecules A and B (MICA/B) presented on tumor cells and is key to trigger the cytolytic response of NK cells. However, tumors have developed sophisticated strategies to evade NK cell surveillance, which lead to failure of tumor immunotherapy. In this paper, we summarized these immune escaping strategies, including the downregulation of ligands for activating receptors, upregulation of ligands for inhibitory receptors, secretion of immunosuppressive compounds, and the development of apoptosis resistance. Then, we focus on recent advancements in NK cell immune therapies, which include engaging activating NK cell receptors, upregulating NKG2D ligand MICA/B expression, blocking inhibitory NK cell receptors, adoptive NK cell therapy, chimeric antigen receptor (CAR)-engineered NK cells (CAR-NK), and NKG2D CAR-T cells, especially several vaccines targeting MICA/B. This review will inspire the research in NK cell biology in tumor and provide significant hope for improving cancer treatment outcomes by harnessing the potent cytotoxic activity of NK cells.
Epcoritamab monotherapy in patients with relapsed or refractory follicular lymphoma (EPCORE NHL-1): a phase 2 cohort of a single-arm, multicentre study
Authors: Kim M Linton, Umberto Vitolo, Wojciech Jurczak, Pieternella J Lugtenburg, Emmanuel Gyan, Anna Sureda, Jacob Haaber Christensen, Brian Hess, Hervé Tilly, Raul Cordoba, David John Lewis, Craig Okada, Martin Hutchings, Michael Roost Clausen, Juan-Manuel Sancho, Tara Cochrane, Sirpa Leppä, Martine E D Chamuleau, Diana Gernhardt, Işıl Altıntaş, Yan Liu, Tahamtan Ahmadi, Minh H Dinh, Daniela Hoehn, Elena Favaro, Brian Elliott, Catherine Thieblemont, Julie M Vose
Journal: The Lancet Haematology (2024)
Learnings from the first AI-enabled skin cancer device for primary care authorized by FDA
Authors: Kaushik P. Venkatesh, Kushal T. Kadakia, Stephen Gilbert
Journal: npj Digital Medicine (2024)

The U.S. Food and Drug Administration's (FDA) recent authorization of DermaSensor, an AI-enabled device for skin cancer detection in primary care, marks a pivotal moment in digital health innovation. Clinically, the authorization of the first AI-enabled device for use by non-specialists for detecting skin cancer reinforces the feasibility of digital health technologies to bridge gaps in access and expertise in medical practice. The authorization also establishes a new regulatory precedent for FDA authorization of medical devices incorporating AI and machine learning (ML) technologies within dermatology. Together, this article uses the DermaSensor authorization to examine the clinical evidence and regulatory implications of emerging AI-enabled technologies in dermatology.
Exergaming and cognitive functions in people with mild cognitive impairment and dementia: a meta-analysis
Authors: Joyce Y. C. Chan, Jiani Liu, Aaron T. C. Chan, Kelvin K. F. Tsoi
Journal: npj Digital Medicine (2024)

Abstract Exergaming is a combination of exercise and gaming. Evidence shows an association between exercise and cognition in older people. However, previous studies showed inconsistent results on the cognitive benefits of exergaming in people with cognitive impairment. Therefore, this study aims to examine the effect of exergaming intervention on cognitive functions in people with MCI or dementia. A systematic literature search was conducted via OVID databases. Randomized controlled trials (RCTs) examined the effect of an exergaming intervention on cognitive functions in people with MCI or dementia were included. Subgroup analyses were conducted according to the type of intervention and training duration. Twenty RCTs with 1152 participants were identified, including 14 trials for MCI and 6 trials for dementia. In people with MCI, 13 studies used virtual-reality (VR)-based exergaming. Those who received VR-based exergaming showed significantly better global cognitive function [SMD (95%CI) = 0.67 (0.23–1.11)], learning and memory [immediate recall test: 0.79 (0.31–1.27); delayed recall test: 0.75 (0.20–1.31)], working memory [5.83 (2.27–9.39)], verbal fluency [0.58 (0.12–1.03)], and faster in executive function than the controls. For people with dementia, all studies used video-based exergaming intervention. Participants with exergaming intervention showed significantly better global cognitive function than the controls [0.38 (0.10–0.67)]. Subgroup analyses showed that longer training duration generated larger effects. The findings suggest that exergaming impacts cognitive functions in people with MCI and dementia. Cognitive benefits are demonstrated for those with a longer training duration. With technological advancement, VR-based exergaming attracts the attention of people with MCI and performs well in improving cognitive functions.
Author Correction: Effectiveness of DialBetesPlus, a self-management support system for diabetic kidney disease: Randomized controlled trial
Authors: Kayo Waki, Mitsuhiko Nara, Syunpei Enomoto, Makiko Mieno, Eiichiro Kanda, Akiko Sankoda, Yuki Kawai, Kana Miyake, Hiromichi Wakui, Yuya Tsurutani, Nobuhito Hirawa, Tadashi Yamakawa, Shiro Komiya, Akihiro Isogawa, Shinobu Satoh, Taichi Minami, Tamio Iwamoto, Tatsuro Takano, Yasuo Terauchi, Kouichi Tamura, Toshimasa Yamauchi, Masaomi Nangaku, Naoki Kashihara, Kazuhiko Ohe
Journal: npj Digital Medicine (2024)