tbuckley/cabot-search huggingface dataset:
Multiplex genome editing eliminates lactate production without impacting growth rate in mammalian cells
Authors: Hooman Hefzi, Iván Martínez-Monge, Igor Marin de Mas, Nicholas Luke Cowie, Alejandro Gomez Toledo, Soo Min Noh, Karen Julie la Cour Karottki, Marianne Decker, Johnny Arnsdorf, Jose Manuel Camacho-Zaragoza, Stefan Kol, Sanne Schoffelen, Nuša Pristovšek, Anders Holmgaard Hansen, Antonio A. Miguez, Sara Petersen Bjørn, Karen Kathrine Brøndum, Elham Maria Javidi, Kristian Lund Jensen, Laura Stangl, Emanuel Kreidl, Thomas Beuchert Kallehauge, Daniel Ley, Patrice Ménard, Helle Munck Petersen, Zulfiya Sukhova, Anton Bauer, Emilio Casanova, Niall Barron, Johan Malmström, Lars K. Nielsen, Gyun Min Lee, Helene Faustrup Kildegaard, Bjørn G. Voldborg, Nathan E. Lewis
Journal: Nature Metabolism (2025)
Multi-omic quantitative trait loci link tandem repeat size variation to gene regulation in human brain
Authors: Ya Cui, Frederick J. Arnold, Jason Sheng Li, Jie Wu, Dan Wang, Julien Philippe, Michael R. Colwin, Sebastian Michels, Chaorong Chen, Tamer Sallam, Leslie M. Thompson, Albert R. La Spada, Wei Li
Journal: Nature Genetics (2025)
Association of a remote monitoring programme with all‐cause mortality and hospitalizations in patients with heart failure: National‐scale, real‐world evidence from a 3‐year propensity score analysis of the <scp>TELESAT</scp>‐<scp>HF</scp> study
Authors: Nicolas Girerd, Vanessa Barbet, Marie‐France Seronde, Hélène Benchimol, Annabelle Jagu, Jean‐Michel Tartière, Olivier Hanon, François Picard, Stéphane Lafitte, Magali Lemaitre, Nicolas Pages, Sophie Nisse‐Durgeat, Patrick Jourdain
Journal: European Journal of Heart Failure (2025)
Humanized In Vivo Bone Tissue Engineering: In Vitro Preculture Conditions Control the Structural, Cellular, and Matrix Composition of Humanized Bone Organs (Adv. Healthcare Mater. 2/2025)
Authors: Agathe Bessot, Flavia Medeiros Savi, Jennifer Gunter, Jayanti Mendhi, Shahrouz Amini, David Waugh, Jacqui McGovern, Dietmar W. Hutmacher, Nathalie Bock
Journal: Advanced Healthcare Materials (2025)
Dye‐Based Fluorescent Organic Nanoparticles, New Promising Tools for Optogenetics (Adv. Healthcare Mater. 2/2025)
Authors: Jérémy Lesas, Thomas C.M. Bienvenu, Eleonore Kurek, Jean‐Baptiste Verlhac, Zoé Grivet, Maude Têtu, Delphine Girard, Frédéric Lanore, Mireille Blanchard‐Desce, Cyril Herry, Jonathan Daniel, Cyril Dejean
Journal: Advanced Healthcare Materials (2025)
Chlorin e6‐Loaded Nanostructured Lipid Carriers Targeted by Angiopep‐2: Advancing Photodynamic Therapy in Glioblastoma (Adv. Healthcare Mater. 2/2025)
Authors: Carlotta Pucci, Daniele De Pasquale, Andrea Degl'Innocenti, Margherita Montorsi, Andrea Desii, Marta Pero, Chiara Martinelli, Martina Bartolucci, Andrea Petretto, Gianni Ciofani
Journal: Advanced Healthcare Materials (2025)
Eumelanin‐Enhanced Photothermal Disinfection of Contact Lenses Using a Sustainable Marine Nanoplatform Engineered with Electrospun Nanofibers (Adv. Healthcare Mater. 2/2025)
Authors: Magdalena Bartolewska, Alicja Kosik‐Kozioł, Zbigniew Korwek, Zuzanna Krysiak, Devis Montroni, Maciej Mazur, Giuseppe Falini, Filippo Pierini
Journal: Advanced Healthcare Materials (2025)
Macrophage Membrane‐Biomimetic Multi‐Layered Nanoparticles Targeting Synovial Angiogenesis for Osteoarthritis Therapy (Adv. Healthcare Mater. 2/2025)
Authors: Junyi Liao, Zhenglin Zhu, Jing Zou, Senrui Liu, Xuefeng Luo, Wei Bao, Chengcheng Du, Yiting Lei, Wei Huang
Journal: Advanced Healthcare Materials (2025)
Pancreatic cancer-derived extracellular vesicles remodel the tumor microenvironment and enhance chemoresistance by delivering KRASG12D protein to cancer-associated fibroblasts
Authors: Xinyuan Liu, Jiaqi Yang, Sicong Huang, Yifan Hong, Yupeng Zhu, Jianing Wang, Yi Wang, Tingbo Liang, Xueli Bai
Journal: Molecular Therapy (2025)
Tumor‐Resident Intracellular Bacteria Scavenger Activated In Situ Vaccines for Potent Cancer Photoimmunotherapy
Authors: Bai Lv, Yifan Zhao, Gang Li, Huimei Jiang, Min Zhang, Zequn Li, Jie Cao
Journal: Advanced Healthcare Materials (2025)

Abstract In situ tumor vaccines, which utilize antigens generated during tumor treatment to stimulate a cancer patient's immune system, has become a potential field in cancer immunotherapy. However, due to the immunosuppressive tumor microenvironment (ITME), the generation of tumor antigens is always mild and not sufficient. Tumor‐resident intracellular bacteria have been identified as a complete tumor microenvironment component to contribute to creating ITME. Herein, a tumor‐resident intracellular bacteria scavenger is designed to induce enhanced antitumor photoimmunotherapy‐driven in situ vaccines for treating hypoxic tumors. This scavenger is developed by integrating photosensitizer CyI and antibiotics Doxycycline (Doxy) into thermal‐sensitive tumor‐derived exosomes fused liposomes (ECDL). In vitro and in vivo results showed that ECDL could homologous target to cancer cells and restrict the respiration of mitochondrial to reduce tumor hypoxia, thus providing continuous oxygen to eliminate both tumor cells and tumor‐resident intracellular bacteria, which could induce in situ vaccines for ablating the primary tumor and inhibiting the tumor metastasis and recurrence. Moreover, eliminating tumor‐resident intracellular bacteria neutralizes the ITME and triggers the production of bacterial‐related neoantigens, which could further strength the immunotherapy. This study provided versatile and effective in situ vaccines that are promising for local, abscopal, and metastatic tumor treatment.
Incidentally Diagnosed Endolymphatic Sac Tumor on 68Ga-DOTA-TATE PET/CT in a Suspected Case of Pheochromocytoma
Authors: Harini Koorma, Mohan Roop Jayanthi, Sudhakar Vengala, Ajay Varun Reddy
Journal: Clinical Nuclear Medicine (2025)

Abstract Endolymphatic sac tumors (ELSTs) are rare, slow-growing, and locally aggressive neoplasms that originate from the epithelial lining of the endolymphatic duct and sac. These are characterized by their infiltrative growth pattern and the potential for local destruction of surrounding structures, including the inner ear and temporal bone. We report a case of an incidentally diagnosed sporadic ELST. The article provides an overview of the relevant literature on this topic and discusses the clinical, radiological, and surgical findings pertaining to this particular ELST case. Given their indolent growth pattern and lack of significant changes on interval imaging, surgical intervention is often deferred for these lesions.
Developmental Venous Anomaly
Authors: Pei Ing Ngam, Yoshimi Anzai, Satoshi Minoshima
Journal: Clinical Nuclear Medicine (2025)

Abstract Developmental venous anomaly (DVA) is a common benign brain vascular malformation, typically asymptomatic, with intervention required when complications arise. We present the case of a 63-year-old man with mild cognitive decline whose brain FDG PET showed decreased uptake not only at the site of DVA, but in the extended surrounding parenchyma where MRI showed no parenchymal abnormalities. DVAs arise from developmental irregularities in the embryonic venous system, but likely affecting a greater extent of the surrounding parenchymal functions. These findings highlight the clinical significance of DVAs and their effects on the parenchymal function seen on FDG PET albeit normal parenchyma on structural imaging.
The Value of Multidisciplinary Neuro-oncological Tumor Boards to Increase the Accuracy of FET PET for Identifying Brain Tumor Relapse
Authors: Garry S. Ceccon, Jan-Michael Werner, Maximilian I. Ruge, Roland Goldbrunner, Eren Celik, Christian Baues, Martina Deckert, Anna Brunn, Manuel Montesinos Rongen, Reinhard Büttner, Veronika Dunkl, Lucia Nogova, Marc Schlamann, Christoph Kabbasch, Daniel Rueß, Jürgen Hampl, Michael M. Wollring, Elena K. Rosen, Caroline Tscherpel, Gabriele Stoffels, Philipp Lohmann, Felix M. Mottaghy, Gereon R. Fink, Karl-Josef Langen, Norbert Galldiks
Journal: Clinical Nuclear Medicine (2025)

Purpose Especially in Europe, amino acid PET is increasingly integrated into multidisciplinary neuro-oncological tumor boards (MNTBs) to overcome diagnostic uncertainties such as treatment-related changes. We evaluated the accuracy of MNTB decisions that included the O -(2-[ 18 F]-fluoroethyl)-L-tyrosine (FET) PET information compared with FET PET results alone to differentiate tumor relapse from treatment-related changes. Patients and Methods In a single academic center, we retrospectively evaluated 180 MNTB decisions of 151 patients with CNS WHO grade 3 or 4 gliomas (n = 122) or brain metastases (n = 29) presenting equivocal MRI findings following anticancer treatment. All patients underwent FET PET imaging besides MRI before MNTB discussion. Additionally, the patient’s clinical status and pretreatment were considered for decision-making. The diagnostic performance was calculated for FET PET findings alone and MNTB decisions that included FET PET results using 2 × 2 contingency tables. MNTB decisions were validated using the neuropathological result in 43% (n = 78) or clinicoradiologically in 57% (n = 102). Results FET PET results alone yielded an accuracy of 87% (sensitivity, 90%; specificity, 65%; positive predictive value, 95%). When integrating FET PET results for decision-making in the MNTB setting, the accuracy increased to 95% (sensitivity, 99%; specificity, 70%; positive predictive value, 96%; P = 0.002). In MNTB decisions concerning glioblastoma patients, the median survival was 2.4 times longer when FET PET suggested treatment-related changes (15.6 vs 6.4 months; P = 0.009). Conclusions Our results suggest that MNTB discussion further enhances the FET PET value for identifying brain tumor relapse. A prospective evaluation of FET PET results with and without integration in an MNTB is warranted.
Gastrin-Releasing Peptide Receptor Targeting PET/CT With 68Ga-NOTA-RM26 in the Assessment of Glioma and Combined Multiregional Biopsies
Authors: Rongxi Wang, Yilin Li, Ziyang Li, Jiarou Wang, Linlin Li, Jialin Xiang, Chenhao Jia, Xingtong Peng, Yu Wang, Wenbin Ma, Li'ao Wang, Wang Jia, Xiaoyuan Chen, Deling Li, Zhaohui Zhu, Jingjing Zhang
Journal: Clinical Nuclear Medicine (2025)

Purpose The aim of this study was to investigate the value of 68 Ga-NOTA-RM26 ( 68 Ga-RM26), a gastrin-releasing peptide receptor–targeting antagonist labeled with the radionuclide 68 Ga, in the diagnosis of high-grade gliomas and in combination with multiregional biopsies using PET/CT. Patients and Methods After institutional review board approval and informed consent, a total of 35 patients with suspected glioma lesions were enrolled in this study. All patients underwent 68 Ga-RM26 PET/CT scans within 2 weeks before surgery. Results There were 8 grade II gliomas, 6 grade III gliomas, and 18 grade IV gliomas in a total of 32 glioma lesions. 68 Ga-RM26 PET/CT diagnosed 74.4% of lesions (27/32) of all glioma tumor types, and almost all high-grade gliomas were successfully diagnosed (23/24, 95.8%). Among the 9 negative glioma lesions, there were 8 low-grade gliomas (grade II). There was a significantly higher SUV max , SUV mean , and the lesion-to-background ratio (T/B ratio) in high-grade gliomas compared with low-grade gliomas ( P &lt; 0.001). In addition, there was a high correlation between the immunohistochemical staining score of gliomas and parameters (SUV max , SUV mean , and T/B ratio) on 68 Ga-RM26 PET/CT ( P &lt; 0.001), and verified by immunohistochemical staining on multiple-point samples of glioma lesions guided by 68 Ga-RM26 PET/CT. Conclusions 68 Ga-RM26 could noninvasively diagnose high-grade gliomas and be a promising PET tracer for predicting glioma grading before surgery. This pilot study indicated that the uptake of 68 Ga-RM26 correlates with WHO grade in glioma, and preoperative 68 Ga-RM26 PET/CT may be helpful to guide multiple-point biopsy of gliomas.
Understanding tinnitus symptom dynamics and clinical improvement through intensive longitudinal data
Authors: Milena Engelke, Jorge Piano Simões, Laura Basso, Nina Wunder, Berthold Langguth, Thomas Probst, Rüdiger Pryss, Winfried Schlee
Journal: npj Digital Medicine (2025)

Abstract Intensive longitudinal sampling enhances subjective data collection by capturing real-time, dynamic inputs in natural settings, complementing traditional methods. This study evaluates the feasibility of using daily self-reported app data to assess clinical improvement among tinnitus patients undergoing treatment. App data from a multi-center randomized clinical trial were analysed using time-series feature extraction and nested cross-validated ordinal regression with elastic net regulation to predict clinical improvement based on the Clinical Global Impression—Improvement scale (CGI-I). With 50% app compliance ( N = 129, 8480 entries), the model demonstrated good fit to the test data (McFadden R2 = 0.82) suggesting its generalizability. Clinical improvement was associated with linear declines in tinnitus-related thoughts, jaw tension, tinnitus loudness, increases in happiness, and variability changes in tinnitus loudness and distress. These findings suggest that daily self-reported data on tinnitus symptoms is sensitive to treatment response and provides insights into specific symptom changes that occur during treatment.
Redefining Ketamine Pharmacology for Antidepressant Action: Synergistic NMDA and Opioid Receptor Interactions?
Authors: Marjorie R. Levinstein, Reece C. Budinich, Jordi Bonaventura, Alan F. Schatzberg, Carlos A. Zarate, Michael Michaelides
Journal: American Journal of Psychiatry (2025)

Ketamine is a racemic compound and medication comprised of (S)-ketamine and (R)-ketamine enantiomers and its metabolites. It has been used for decades as a dissociative anesthetic, analgesic, and recreational drug. More recently, ketamine, its enantiomers, and its metabolites have been used or are being investigated for the treatment of refractory depression, as well as for comorbid disorders such as anxiety, obsessive-compulsive, and opioid use disorders. Despite its complex pharmacology, ketamine is referred to as an N-methyl-d-aspartate (NMDA) receptor antagonist. In this review, the authors argue that ketamine's pharmacology should be redefined to include opioid receptors and the endogenous opioid system. They also highlight a potential mechanism of action of ketamine for depression that is attributed to bifunctional, synergistic interactions involving NMDA and opioid receptors.
Distinct Behavioral Profiles and Neuronal Correlates of Heroin Vulnerability Versus Resiliency in a Multi-Symptomatic Model of Heroin Use Disorder in Rats
Authors: Brittany N. Kuhn, Nazzareno Cannella, Ayteria D. Crow, Veronica Lunerti, Arkobrato Gupta, Stephen J. Walterhouse, Carter Allen, Reda M. Chalhoub, Eric Dereschewitz, Analyse T. Roberts, Mackenzie Cockerham, Angela Beeson, Rusty W. Nall, Abraham A. Palmer, Gary Hardiman, Leah C. Solberg Woods, Dongjun Chung, Roberto Ciccocioppo, Peter W. Kalivas
Journal: American Journal of Psychiatry (2025)

The behavioral and diagnostic heterogeneity within the opioid use disorder (OUD) diagnosis is not readily captured in current animal models, limiting the translational relevance of the mechanistic research that is conducted in experimental animals. The authors hypothesized that a nonlinear clustering of OUD-like behavioral traits would capture population heterogeneity and yield subpopulations of OUD vulnerable rats with distinct behavioral and neurocircuit profiles. Over 900 male and female heterogeneous stock rats, a line capturing genetic and behavioral heterogeneity present in humans, were assessed for several measures of heroin use and rewarded and non-rewarded seeking behaviors. A nonlinear stochastic block model clustering analysis was used to assign rats to OUD vulnerable, intermediate, and resilient clusters. Additional behavioral tests and circuit analyses using c-fos protein activation were conducted on the vulnerable and resilient subpopulations. OUD vulnerable rats exhibited greater heroin taking and seeking behaviors relative to those in the intermediate and resilient clusters. Akin to human OUD diagnosis, further vulnerable rat subclustering revealed subpopulations with different combinations of behavioral traits, including sex differences. Lastly, heroin cue-induced neuronal patterns of circuit activation differed between resilient and vulnerable phenotypes. Behavioral sex differences were recapitulated in patterns of circuitry activation, including preferential engagement of extended amygdala stress circuitry in males and cortico-striatal drug cue-seeking circuitry in females. Using a nonlinear clustering approach in rats, the analysis captured behavioral diagnostic heterogeneity reflective of human OUD diagnosis. OUD vulnerability and resiliency were associated with distinct neuronal activation patterns, posing this approach as a translational tool in assessing neurobiological mechanisms underpinning OUD.
Five-Year Outcomes of a School-Based Personality-Focused Prevention Program on Adolescent Substance Use Disorder: A Cluster Randomized Trial
Authors: Patricia Conrod, Sherry H. Stewart, Jean Seguin, Robert Pihl, Benoit Masse, Sean Spinney, Samantha Lynch
Journal: American Journal of Psychiatry (2025)

Rates of substance use disorders (SUDs) remain significantly above national targets for health promotion and disease prevention in Canada and the United States. This study investigated the 5-year SUD outcomes following a selective drug and alcohol prevention program targeting personality risk factors for adolescent substance misuse. The Co-Venture trial is a cluster randomized trial involving 31 high schools in the greater Montreal area that agreed to conduct annual health behavior surveys for 5 years on the entire 7th grade cohort of assenting students enrolled at the school in 2012 or 2013. Half of all schools were randomly assigned to be trained and assisted in the delivery of the personality-targeted PreVenture Program to all eligible 7th grade participants. The intervention consisted of a brief (two-session) group cognitive-behavioral intervention that is delivered in a personality-matched fashion to students who have elevated scores on one of four personality traits linked to early-onset substance misuse: impulsivity, sensation seeking, anxiety sensitivity, or hopelessness. Mixed-effects multilevel Bayesian models were used to estimate the effect of the intervention on the year-by-year change in probability of SUD. When baseline differences were controlled for, a time-by-intervention interaction revealed positive growth in SUD rate for the control group (b=1.380, SE=0.143, odds ratio=3.97) and reduced growth for the intervention group (b=-0.423, SE=0.173, 95% CI=-0.771, -0.084, odds ratio=0.655), indicating a 35% reduction in the annual increase in SUD rate in the intervention condition relative to the control condition. Group differences in SUD rates were reliably nonzero (95% confidence) at the fourth and fifth year of assessment. Secondary analyses revealed no significant intervention effects on growth of anxiety, depression, or total mental health difficulties over the four follow-up periods. This study showed for the first time that personality-targeted interventions might protect against longer-term development of SUD.
Post‐traumatic stress disorder: evolving conceptualization and evidence, and future research directions
Authors: Chris R. Brewin, Lukoye Atwoli, Jonathan I. Bisson, Sandro Galea, Karestan Koenen, Roberto Lewis‐Fernández
Journal: World Psychiatry (2025)

The understanding of responses to traumatic events has been greatly influenced by the introduction of the diagnosis of post‐traumatic stress disorder (PTSD). In this paper we review the initial versions of the diagnostic criteria for this condition and the associated epidemiological findings, including sociocultural differences. We consider evidence for post‐traumatic reactions occurring in multiple contexts not previously defined as traumatic, and the implications that these observations have for the diagnosis. More recent developments such as the DSM‐5 dissociative subtype and the ICD‐11 diagnosis of complex PTSD are reviewed, adding to evidence that there are several distinct PTSD phenotypes. We describe the psychological foundations of PTSD, involving disturbances to memory as well as to identity. A broader focus on identity may be able to accommodate group and communal influences on the experience of trauma and PTSD, as well as the impact of resource loss. We then summarize current evidence concerning the biological foundations of PTSD, with a particular focus on genetic and neuroimaging studies. Whereas progress in prevention has been disappointing, there is now an extensive evidence supporting the efficacy of a variety of psychological treatments for established PTSD, including trauma‐focused interventions – such as trauma‐focused cognitive behavior therapy (TF‐CBT) and eye movement desensitization and reprocessing (EMDR) – and non‐trauma‐focused therapies, which also include some emerging identity‐based approaches such as present‐centered and compassion‐focused therapies. Additionally, there are promising interventions that are neither psychological nor pharmacological, or that combine a pharmacological and a psychological approach, such as 3,4‐methylenedioxymethamphetamine (MDMA)‐assisted psychotherapy. We review advances in the priority areas of adapting interventions in resource‐limited settings and across cultural contexts, and of community‐based approaches. We conclude by identifying future directions for work on trauma and mental health.
Assessing and Addressing Cardiovascular and Obstetric Risks in Patients Undergoing Assisted Reproductive Technology: A Scientific Statement From the American Heart Association
Authors: Rina Mauricio, Garima Sharma, Jennifer Lewey, Rose Tompkins, Torie Plowden, Kathryn Rexrode, Mary Canobbio, Jenna Skowronski, Afshan Hameed, Candice Silversides, Harmony Reynolds, Arthur Vaught
Journal: Circulation (2025)

The use of assisted reproductive technology (ART) is growing, both to assist individuals with infertility and for fertility preservation. Individuals with cardiovascular disease (CVD), or risk factors for CVD, are increasingly using ART. Thus, knowing how to care for patients undergoing ART is important for the cardiovascular clinician. In this scientific statement, we review the ART process and known short-term and long-term risks associated with ART that can adversely affect patients with CVD. We review current knowledge on risks from ART for specific cardiac conditions and provide a suggested approach to evaluating and counseling patients with CVD contemplating ART as well as suggested management before and during the ART process. Individuals with CVD are at increased risk for pregnancy complications, and management of this unique population has been discussed previously. The focus of this scientific statement is on ART. Therefore, discussions on risk assessment, counseling, and management of individuals with CVD during pregnancy are limited, and established guidelines are referenced.