tbuckley/cabot-search huggingface dataset:
Loss of RPN1 Promotes Antitumor Immunity via PD-L1 Checkpoint Blockade in Triple-negative Breast Cancer——Experimental Studies
Authors: Mengxue Wang, Xunjia Li, Yushen Wu, Long Wang, Xue Zhang, Meng Dai, Yang Long, Deyu Zuo, Shengwei Li, Xuedong Yin
Journal: International Journal of Surgery (2024)

Background: RPN1, also known as ribophorin I (RPN1), is a type I transmembrane protein that plays an important role in glycosylation. However, the effects of RPN1 on cancer progression and immune evasion in breast cancer (BC) have not been identified. Materials and methods: The expression of RPN1 was evaluated using RT-qPCR and immunohistochemistry (IHC). The effects of RPN1 on tumor cells were assessed using RT-qPCR, western blotting, flow cytometry, Cell Counting Kit 8 (CCK-8), colony formation assays, and in vivo experiments. The mechanism by which RPN1 modifies programmed death ligand-1 (PD-L1) and the tumor microenvironment was examined by RT-qPCR, western blotting, co-immunoprecipitation (Co-IP), and flow cytometry. The influence of the transcription factor YY1 on RPN1 expression was revealed using bioinformatics analysis, RT-qPCR, and dual-luciferase reporter and chromatin immunoprecipitation (ChIP) assays. Results: RPN1 is aberrantly expressed in triple-negative breast cancer (TNBC) cells, correlating with increased proliferation and poor prognosis. RPN1 mediates the post-translational modification of PD-L1, enhancing its glycosylation and stability, thus facilitating PD-L1-mediated immune escape and tumor growth. The deletion of RPN1 improves the TNBC microenvironment and enhances the efficacy of anti-PD-1 therapy. Additionally, we uncovered a novel regulatory axis involving YY1/RPN1/YBX1 in PD-L1 regulation, affecting TNBC growth and metastasis. Conclusions: Our preliminary study reveals that targeting RPN1 promotes immune suppression, providing a new potential immunotherapy strategy for TNBC. However, further research is necessary to fully elucidate and understand the specific mechanisms of RPN1 in TNBC and its potential for clinical application .
Tibial cortex transverse transport surgery to treat diabetic foot ulcerations: what mechanism is involved in accelerated wound healing?
Authors: JinXiang Shang, Lin Cheng, Qifeng Ou
Journal: International Journal of Surgery (2024)

aDepartment of Orthopedics, The Affiliated Hospital of Shaoxing University, Shaoxing City, Zhejiang Province, China bRegenerative Medicine Institute (REMEDI) at CÚRAM SFI Research Centre for Medical Devices, School of Medicine, College of Medicine, Nursing and Health Sciences, University of Galway, Galway City, Galway County, Ireland *Corresponding author. Address: Regenerative Medicine Institute (REMEDI) at CÚRAM SFI Research Centre for Medical Devices, School of Medicine, College of Medicine, Nursing and Health Sciences, University of Galway, Biomedical Sciences Building, University of Galway, Upper Newcastle, Galway City, Galway County, Ireland, H91 W2TY, E-mail: [email protected]; [email protected] (L. Cheng and Q. Ou). This is an open access article distributed under the Creative Commons Attribution-ShareAlike License 4.0, which allows others to remix, tweak, and build upon the work, even for commercial purposes, as long as the author is credited and the new creations are licensed under the identical terms.
Letter to editor: addressing methodological limitations in the meta-analysis of hospital volume and postoperative mortality after esophagectomy
Authors: Qiang Yi, Gangfeng Zhu, Jinghua Zhong
Journal: International Journal of Surgery (2024)

aThe First Clinical Medical College, Gannan Medical University, Ganzhou, Jiangxi Province, China bDepartment of Oncology, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi Province, China Corresponding author. Address: Department of Oncology, The First Affiliated Hospital of Gannan Medical University, 128 Jinling Road, Ganzhou, Jiangxi Province, China, 341000, E-mail: [email protected]. (J. Zhong). See the article "Hospital Surgical Volume and Postoperative Mortality in Esophagectomy: A Systematic Review and Meta-analysis" in volume 110(5): 3021–3029. This is an open access article distributed under the Creative Commons Attribution-ShareAlike License 4.0, which allows others to remix, tweak, and build upon the work, even for commercial purposes, as long as the author is credited and the new creations are licensed under the identical terms.
Letter to the Editor: comment on “Association of varicose veins with incidence risk of atrial fibrillation: a population-based cohort study”
Authors: Min Wang, Lei Wang, Xingchen Wang, Hongshuo Shi, Huimin Lu
Journal: International Journal of Surgery (2024)

aShandong University of Traditional Chinese Medicine, Jinan, Shandong, China bThe Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China cDepartment of Vascular Surgery, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China *Corresponding authors. Address: The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China. E-mail: [email protected] (X. Wang); Department of Vascular Surgery, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China. E-mails: [email protected] (H. Lu); [email protected] (H. Shi). #Equal contribution. Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article. This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The efficacy of adjuvant chemotherapy for curative resected biliary tract cancers: a systematic review and network meta-analysis of randomized clinical trials
Authors: Yishan Peng, Aijun Liang, Zhi Chen, Bin Yang, Wenke Yu, Jingduo Deng, Yu Fu, Yu Nie, Yuan Cheng
Journal: International Journal of Surgery (2024)

Background: Despite complete resection, recurrence rate of biliary duct cancer (BTC) remains high, leading to poor prognosis. Postoperative adjuvant chemotherapy (ACT) following radical resection may substantially reduce the recurrence risk by eradicating micrometastatic lesions. However, the benefits of postoperative ACT and the optimal ACT strategy are still unclear for BTC. The objectives of this study are to evaluate the prognostic value of ACT and compare the effectiveness of different ACTs among BTC patients after curative resection. Methods: A comprehensive literature search was conducted across PubMed, Cochrane Library, Web of Science and EMBASE database to identify randomized controlled trials (RCTs) comparing the benefits of ACT versus no intervention or other ACTs in BTC patients after curative resection. A random-effects network meta-analysis was performed to compare overall survival (OS) and relapse-free survival (RFS). The quality of evidence was rated using the GRADE-framework. Results: Eight RCTs comprising 1803 patients were included in the meta-analysis. ACT was associated with significant improvements in 5-year all-cause mortality (4 RCTs, HR 0.93; 95%CI 0.87-1.00, marginally significant; low-certainty evidence), RFS (5 RCTs, HR 0.87; 95%CI 0.78-0.98; moderate-certainty evidence) and OS (7 studies, HR 0.85; 95%CI 0.75-0.96; low-certainty evidence) compared with observation. ACT had significantly better survival benefits on patients with negative margin(R0), lymph node positive (N +) and TNM-stage I/II (P &lt; 0.05). Further network meta-analysis demonstrated fluorouracil-based ACT was significantly inferior to gemcitabine-based ACT (HR 1.20, 95%CI 1.10-1.25) in improving RFS. However, both were superior to observation (P &lt; 0.05). No statistical difference in OS was observed between gemcitabine-based and fluorouracil-based chemotherapy (HR 1.00, 95%CI 0.86-1.20). In subgroup analysis, fluorouracil-based ACT but not gemcitabine-based ACT achieved significantly better OS benefits on patients with N + (HR 0.67; 95%CI 0.52-0.86) and R0 (HR 0.69; 95%CI 0.54-0.88). Conclusion: Compared with observation, ACT should be routinely recommended to improve survival outcomes in BTC patients after curative resection, especially for those with R0, N + and TNM stage I/II. Gemcitabine-based ACT performed better than other chemotherapies on improving RFS. This network meta-analysis provides precise information for determining the best adjuvant treatment for resected BTC. Further thorough and high quality RCTs are needed.
European recommendations from health care professionals and people living with obesity on safe practice for bariatric and metabolic surgery medical tourism: a modified delphi consensus statement from EASO, IFSO-EC and ECPO
Authors: Laurence J Dobbie, Susie Birney, Cathy Breen, Sheree Bryant, Ken Clare, Andreea Ciudin, Daniel M Felsenreich, Jason C G Halford, Helen Heneghan, Nicola Di Lorenzo, Vicki Mooney, Chetan Parmar, Jean O’Connell, Grace O’Malley, Euan Woodward, Volkan D Yumuk, Ralph Peterli, Barbara McGowan, BMT Consensus Group
Journal: International Journal of Surgery (2024)

Background Bariatric and metabolic surgery tourism (BMT) is becoming an increasingly popular route to treatment for patients living with obesity. Recent reports have highlighted that some patients travelling abroad for bariatric surgery have received inadequate care, fraudulent care, and, tragically, some cases have resulted in death. This study aimed to define consensus in Europe regarding safe practices concerning BMT. Materials and methods IFSO-EC, EASO and ECPO initiated a task force to delineate safe practices in BMT. Two expert European panels were convened, one comprised of healthcare professionals (identified from EASO and IFSO-EC) and the other of patient representatives (identified from ECPO). The study utilised a modified Delphi consensus methodology, and 135 questions were administered. Surveys were conducted anonymously online, and consensus was defined as 70% agreement. Themes analysed regarding BMT included regulation, pre-operative evaluation, operative care, post-operative care, advertising and online information. Results One hundred and nineteen healthcare professionals and 88 patient representatives participated from 26 countries. The healthcare professional panel included 66 bariatric surgeons, 28 endocrinologists, 18 dietitians, three nurses, two psychologists, one general practitioner and one gastroenterologist. Three questionnaire rounds were conducted for the healthcare professional panel, and two were performed for the patient representative panel. Consensus recommendations were given across all themes relevant to BMT. These included evaluating and managing psychological health, sleep apnoea, cardiovascular disease, liver health and dietetic assessment. The recommendations covered the requirements for regulatory standards, including surgeon accreditation and procedural volume. They also included recommendations regarding patient education, standardised operative care, online information provision, and follow-up. Conclusions Through collaboration with healthcare professionals and patients living with obesity, we provide European recommendations regarding safe practices concerning BMT. Further evaluation is required regarding outcomes following BMT. These data, alongside the Delphi consensus recommendations, will inform BMT clinical guideline development. Graphical Abstract
Letter to the editor on the article “long-term Outcomes of Surgical Interventions for Stress Urinary Incontinence: a Systematic Review and Network Meta-analysis” are all SUI slings up to standard? a closer look at effectiveness and bias
Authors: Chia-Min Liu, Yin Chien Ou
Journal: International Journal of Surgery (2024)

Department of Urology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan Corresponding author. Address:Department of Urology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, No.138, Sheng-Li Road, Tainan, Taiwan, E-mail: [email protected] (Y.C. Ou). This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
Substitution of liposomal bupivacaine for lidocaine reduces incidence of injection-emergent adverse events after intraarticular therapies for knee osteoarthritis: a prospective cohort study
Authors: Junqing Lin, Tao Gao, Lenian Zhou, Tiexin Liu, Qiuke Wang, Zixuan Lin, Hongyi Zhu
Journal: International Journal of Surgery (2024)

Abstract Local anesthetics as a part of intraarticular therapies (IATs) are widely used for treating knee osteoarthritis (KOA). Whether substitution of liposomal bupivacaine (LB) for lidocaine is safe and effective in reducing incidence of injection-emergent adverse events after IATs remains unclear. Methods We recruited outpatients who had a clinical diagnosis of KOA and decided to receive IATs from November 2023 to April 2024. The type of IAT (glucocorticoids, platelet-rich plasma and hyaluronic acid) for each participant was decided by the preference of patients after consulting with his or her treating physicians. Using lidocaine or LB as local anesthetics was determined by enrollment timing due to considerations on safety. The primary outcome was injection-emergent adverse events after IATs. Secondary outcome measures included Visual Analogue Scale (VAS) pain scores and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain subscale score. Results In this study, 123 and 103 patients respectively received lidocaine and LB according to their enrollment date. Compared with lidocaine, using LB yielded reduced incidence of AE in the overall two weeks (LB versus lidocaine, 30.1% versus 45.5%, P = 0.018) and Week1 (LB versus lidocaine, 23.3% versus 39.8%, P = 0.008). After adjusting for sex, baseline BMI, age, baseline WOMAC pain subscale score and K-L grade, substitution of LB for lidocaine was significantly associated with the reduced incidence of AE in two weeks (OR [95%CI], 0.484 [0.274-0.853], P = 0.012). In the initial three days, the LB groups reported better outcomes in terms of VAS pain score (change from baseline of VAS pain, LB versus lidocaine, Day 1 − 8.3 ± 8.9 versus − 1.9 ± 9.3, P &lt; 0.001; Day 2 − 20.6 ± 16.1 versus − 13.7 ± 19.4, P = 0.005; Day 3 − 22.3 ± 18.5 versus − 16.3 ± 19.3, P = 0.020). The changes from baseline of WOMAC pain subscale at Day 14 were similar between the two groups (LB versus lidocaine, − 32.2 ± 11.7 versus − 29.4 ± 11.3, P = 0.073). Conclusion With substitution of LB for lidocaine, patient might reported reduced incidence of AEs, mainly derived from the superiority in Week 1. The substitution of LB for lidocaine was safe in different scenarios of IATs and future randomized clinical trials were warranted by the current study.
Tracking adipose-derived stem cell exosomes applied in a mouse crush injury model: insights from fluorescent labeling and spatial transcriptomics – an experimental study
Authors: Cheng-Shyuan Rau, Shao-Chun Wu, Pao-Jen Kuo, Chia-Wei Lin, Tsu-Hsiang Lu, Yi-Chan Wu, Chia-Wen Tsai, Ching-Hua Hsieh
Journal: International Journal of Surgery (2024)

Adipose-derived stem cell exosomes (ADSC-exos) are promising for nerve regeneration; however, their precise mechanisms remain unclear. This study employed fluorescent labeling and spatial transcriptomics to track the effects of ADSC-exos on crushed sciatic nerves in mice. Labeled exosomes were detected in spinal neurons and proximal nerve segments after application. Spatial transcriptomics revealed significant changes in gene expression, with an upregulation of neurons and Schwann cells and the downregulation of oligodendrocytes. The key pathways affected were prosaposin, pleiotrophin, fibroblast growth factor, secreted phosphoprotein 1, SLIT and NTRK-like family, member, vascular endothelial growth factor, and growth arrest-specific protein. ADSC-exo treatment enhanced cell-cell interactions, particularly between Schwann cells and astrocytes, thereby promoting a regenerative environment. Gene ontology analysis suggested improvements in metabolic activity, cell communication, and structural support. This study highlights the complex interplay between multiple cell types and signaling pathways involved in the nerve regeneration response to ADSC-exos. This comprehensive approach offers new perspectives on the role of ADSC-exos in nerve regeneration and paves the way for advanced regenerative strategies for peripheral nerve injuries.
Effects of continuous pecto-intercostal fascial block for management of post-sternotomy pain in patients undergoing cardiac surgery: a randomized controlled trial
Authors: Yanfei Zhao, Dehao He, Wanqing Zhou, Cheng Chen, Zhuoyi Liu, Pingping Xia, Zhi Ye, Chunling Li
Journal: International Journal of Surgery (2024)

Background: Managing postoperative pain following median sternotomy has long been a notable challenge for anesthesiologists. The administration of postoperative analgesia traditionally relies on intravenous pumps for the delivery of opioids. With the development of regional block techniques and postoperative multimodal analgesia, pecto-intercostal fascial block (PIFB) has gained widespread utilization due to its distinctive advantages. However, its application is limited to a single block. This study aimed to indicate whether continuous PIFB analgesia in cardiac surgery via sternotomy could possess clinical advantages compared with intravenous analgesia in terms of postoperative pain management. If continuous PIFB analgesia was the priority, the secondary objective would involve determining the most effective administration method, making it a critical area of exploration. Methods: Totally, 114 patients were randomly allocated to three groups: the PCIA group, receiving intravenous opioid infusion exclusively via pump, and the C-PIFB and I-PIFB groups, where ultrasound-guided PIFB with a nerve blocking pump was administered. The C-PIFB group received a constant basal infusion, while programmed intermittent boluses were administered in the I-PIFB group. The primary endpoint was postoperative visual analogue scale (VAS) scores, and secondary outcomes included intraoperative sufentanil consumption, time to extubation, mobilization, length of stay in intensive care unit (ICU) and hospital, and the incidence of postoperative complications. Results: The VAS scores at rest and during coughing were noticeably diminished in the two block groups relative to the intravenous pump group at 12, 24, 48, and 72 h postoperatively. Notably, intraoperative sufentanil consumption was significantly reduced in the C-PIFB group (3.12 [0.93] ug.kg -1 ) and the I-PIFB group (3.42 [0.77] ug.kg -1 ) compared with the PCIA group (4.66 [1.02] ug.kg -1 , P &lt; 0.001). Time to extubation, mobilization, length of stay in ICU and hospital, and use of rescue analgesics did not exhibit statistically significant differences among the three groups. However, the postoperative complication rates were markedly lower in the C-PIFB group (42.11%) and I-PIFB group (36.84%) relative to the PCIA group (81.58%, P &lt; 0.001). There were no significant differences between C-PIFB and I-PIFB groups regarding VAS score, secondary outcomes, and postoperative complications. Conclusion: Continuous PIFB can provide satisfactory postoperative analgesia while reducing perioperative opioid consumption, diminishing the risk of postoperative complications, and accelerating postoperative recovery for patients undergoing median sternotomy in cardiac surgery. The constant basal infusion method may be the optimal approach for administering continuous PIFB.
Reply letter to the commentary on “Clinical efficacy and biomarker analysis of neoadjuvant camrelizumab plus chemotherapy for early-stage triple-negative breast cancer: a experimental single-arm phase II clinical trial pilot study”
Authors: Xiangjing Meng, Xingchen Meng, Yuechen Wang, Ning Yan, Chunhui Zheng
Journal: International Journal of Surgery (2024)

Meng, Xiangjing; Meng, Xingchen; Wang, Yuechen; Yan, Ning; Zheng, Chunhui Author Information
Nomogram for predicting severe abdominal adhesions prior to definitive surgery in patients with anastomotic fistula post-small intestine resection: a cohort study
Authors: Zheng Yao, Weiwei Shang, Fan Yang, Weiliang Tian, Guoping Zhao, Xin Xu, Risheng Zhao Md, Tao Tian, Wuhan Li, Ming Huang, Yunzhao Zhao, Qian Huang
Journal: International Journal of Surgery (2024)

Background: This study aimed to develop and validate a nomogram for predicting the presence of severe intra-abdominal adhesions before definitive surgery (DS) for anastomotic fistula following small intestine resection (SIR). Methods: Patients were enrolled from January 2009 to October 2023 and were randomly divided (2:1) into development and validation cohorts. Predictors of severe adhesion were identified and integrated into a nomogram. The nomogram’s performance was evaluated through calibration, discrimination, and clinical utility. Results : A total of 414 patients were included, with 276 in the development cohort and 138 in the validation cohort. Severe adhesion was diagnosed in 54 (13%) patients, including 37 (13.4%) in the development cohort and 17 (12.3%) in the validation cohort ( p = 0.76). Five predictors were identified: Sequential Organ Failure Assessment (SOFA) score, duration of early-stage abdominal infection, preoperative albumin (Alb) &lt; 35 g/L, visceral to subcutaneous fat area ratio, and preoperative C-reactive protein (CRP) &gt; 10 mg/L. The nomogram demonstrated robust discrimination, with a Concordance Index (C-index) of 0.80 (95% CI 0.76-0.90) in internal validation, and was well-calibrated. In the validation cohort, the model maintained good discrimination (C-index = 0.79; 95% CI 0.67-0.94) and calibration. Decision curve analysis affirmed the nomogram’s clinical utility. Conclusion: This study introduces a practical nomogram for assessing the risk of severe abdominal adhesion prior to DS in patients undergoing surgery for anastomotic fistula after SIR.
Comparison of thoracoscopic-guided intercostal nerve block and ultrasound-guided intercostal nerve block in postoperative analgesia of uniportal video-assisted lobectomy: a pilot randomized controlled trial
Authors: Yan Li, Luyao Wei, Jian-Hui Du, Jin-Xian He, Xia Xu, Li-Hong Hu
Journal: International Journal of Surgery (2024)

Background: Ultrasound-guided intercostal nerve block (UINB) and thoracoscopic-guided intercostal nerve block (TINB) are often used for analgesia after thoracic surgery. Herein, we compared the application of TINB and UINB for analgesia after uniportal video-assisted lobectomy. Methods: Sixty patients were randomly allocated into two groups: UINB and TINB. The surgical time of intercostal nerve block (INB), the success rate of the first needle, visual analog scale (VAS) scores, the time of the first patient-controlled intravenous analgesia (PCIA) press, the time for removing the thoracic drainage tube, consumption of sufentanil and the number of PCIA presses within 24 hours postoperatively, and adverse reactions (ARs) were compared between the two groups. Results: The surgical time of INB was significantly shorter in the TINB group than in the UINB group ( P &lt; 0.001). The time of the first press of PCIA was significantly earlier in the TINB group than in the UINB group ( P &lt; 0.001). The success rate of the first needle was significantly higher in the TINB group than in the UINB group ( P &lt; 0.001). No significant differences were observed between the two groups regarding VAS scores, time for removing the thoracic drainage tube, the consumption of sufentanil, the number of PCIA presses within 24 hours postoperatively, and ARs. Conclusion: TINB and UINB have similar analgesic effects after uniportal video-assisted lobectomy. However, TINB demonstrates shorter surgical time and a higher success rate than UINB.