tbuckley/cabot-search huggingface dataset:
Duration of viral persistence in human semen after acute viral infection: a systematic review
Authors: Caitlin Pley, Laura Jung, Nadra Nurdin, Tim Venkatesan, Vasanth V Naidu, Rosemary James, Laura Kmentt, Isaac Florence, Ellie Delight, Christina Guo, Alex Paddy Abdel Salam
Journal: The Lancet Microbe (2024)

The persistence of viruses in human semen following acute infection can contribute to the ongoing transmission of a disease or cause resurgence after an outbreak has been declared ended. Viral persistence in semen affects embryonic development and male fertility, and the development of drugs and vaccines. We conducted a systematic review of viral persistence in semen in accordance with PRISMA guidelines. 373 original studies were included in this Review after screening 29 739 articles from five databases. Evidence was found of detection of 22 viruses in human semen following acute infection, including pathogens with pandemic potential. In addition to collating the largest evidence base to date on viral detection in semen following acute infection, this Review reports the maximal and median viral persistence (in days) after the onset of illness and evidence for sexual transmission and viability of the viruses in semen. Finally, the Review presents research gaps that need to be prioritised to guide further study of the dynamics of viral persistence in semen.
Novel human-type receptor-binding H5N1 virus in live poultry markets, China
Authors: Feng Wen, Yu Yang, Yong Li, Jinyue Guo, Zhili Li, Lian Liu, Hao Liu, Kun Mei, Limei Qin, Keshan Zhang, Tao Ren, Shujian Huang
Journal: The Lancet Microbe (2024)
IL-23R is a senescence-linked circulating and tissue biomarker of aging
Authors: Chase M Carver, Sonia L Rodriguez, Elizabeth J Atkinson, Andrew J Dosch, Niels C Asmussen, Paul T Gomez, Ethan A Leitschuh, Jair M Espindola-Netto, Karthik B Jeganathan, Madison G Whaley, Theodore M Kamenecka, Darren J Baker, Andrew J Haak, Nathan K LeBrasseur, Marissa J Schafer
Journal: Nature Aging (2024)

Cellular senescence is an aging mechanism characterized by cell cycle arrest and a senescence-associated secretory phenotype (SASP). Preclinical studies demonstrate that senolytic drugs, which target survival pathways in senescent cells, can counteract age-associated conditions that span several organs. The comparative efficacy of distinct senolytic drugs for modifying aging and senescence biomarkers in vivo has not been demonstrated. Here, we established aging- and senescence-related plasma proteins and tissue transcripts that changed in old versus young female and male mice. We investigated responsivity to acute treatment with venetoclax, navitoclax, fisetin or luteolin versus transgenic senescent cell clearance in aged p16-InkAttac mice. We discovered that age-dependent changes in plasma proteins, including IL-23R, CCL5 and CA13, were reversed by senotherapeutics, which corresponded to expression differences in tissues, particularly in the kidney. In plasma from humans across the lifespan, IL-23R increased with age. Our results reveal circulating factors as candidate mediators of senescence-associated interorgan signal transduction and translationally impactful biomarkers of systemic senescent cell burden.
Stepwise molecular specification of excitatory synapse diversity onto cerebellar Purkinje cells
Authors: Maëla A Paul, Séverine M Sigoillot, Léa Marti, Francisco J Urra Quiroz, Marine Delagrange, Hiu W Cheung, David C Martinelli, Elie Oriol, Vincent Hakim, Philippe Mailly, Fekrije Selimi
Journal: Nature Neuroscience (2024)
Neuropsychiatric polygenic scores are weak predictors of professional categories
Authors: Georgios Voloudakis, Karen Therrien, Simone Tomasi, Veera M. Rajagopal, Shing Wan Choi, Ditte Demontis, John F. Fullard, Anders D. Børglum, Paul F. O’Reilly, Gabriel E. Hoffman, Panos Roussos
Journal: Nature Human Behaviour (2024)
Heterogeneity-driven phenotypic plasticity and treatment response in branched-organoid models of pancreatic ductal adenocarcinoma
Authors: Aristeidis Papargyriou, Mulham Najajreh, David P Cook, Carlo H Maurer, Stefanie Bärthel, Hendrik A Messal, Sakthi K Ravichandran, Till Richter, Moritz Knolle, Thomas Metzler, Akul R Shastri, Rupert Öllinger, Jacob Jasper, Laura Schmidleitner, Surui Wang, Christian Schneeweis, Hellen Ishikawa-Ankerhold, Thomas Engleitner, Laura Mataite, Mariia Semina, Hussein Trabulssi, Sebastian Lange, Aashreya Ravichandra, Maximilian Schuster, Sebastian Mueller, Katja Peschke, Arlett Schäfer, Sophie Dobiasch, Stephanie E Combs, Roland M Schmid, Andreas R Bausch, Rickmer Braren, Irina Heid, Christina H Scheel, Günter Schneider, Anja Zeigerer, Malte D Luecken, Katja Steiger, Georgios Kaissis, Jacco van Rheenen, Fabian J Theis, Dieter Saur, Roland Rad, Maximilian Reichert
Journal: Nature Biomedical Engineering (2024)

In patients with pancreatic ductal adenocarcinoma (PDAC), intratumoural and intertumoural heterogeneity increases chemoresistance and mortality rates. However, such morphological and phenotypic diversities are not typically captured by organoid models of PDAC. Here we show that branched organoids embedded in collagen gels can recapitulate the phenotypic landscape seen in murine and human PDAC, that the pronounced molecular and morphological intratumoural and intertumoural heterogeneity of organoids is governed by defined transcriptional programmes (notably, epithelial-to-mesenchymal plasticity), and that different organoid phenotypes represent distinct tumour-cell states with unique biological features in vivo. We also show that phenotype-specific therapeutic vulnerabilities and modes of treatment-induced phenotype reprogramming can be captured in phenotypic heterogeneity maps. Our methodology and analyses of tumour-cell heterogeneity in PDAC may guide the development of phenotype-targeted treatment strategies.
Multi-omic profiling highlights factors associated with resistance to immuno-chemotherapy in non-small-cell lung cancer
Authors: Yilv Yan, Dongqing Sun, Junjie Hu, Yue Chen, Liangdong Sun, Huansha Yu, Yicheng Xiong, Zhida Huang, Haoran Xia, Xinsheng Zhu, Dongliang Bian, Fenghuan Sun, Likun Hou, Chunyan Wu, Orion R. Fan, Haiyang Hu, An Zeng, Lele Zhang, Yi Eve Sun, Chenfei Wang, Peng Zhang
Journal: Nature Genetics (2024)
Ultrasound-triggered drug release in vivo from antibubble-loaded macrophages
Authors: Omkar Desai, Mario Köster, Doreen Kloos, Nico Lachmann, Hansjörg Hauser, Albert Poortinga, Dagmar Wirth
Journal: Journal of Controlled Release (2024)

Nanoparticles have proven to be attractive carriers in therapeutic drug delivery since they can encapsulate, protect and stabilize a plethora of different drugs, thereby improving therapeutic efficacy and reducing side effects. However, specific targeting of drug-loaded nanoparticles to the tissue of interest and a timely and spatially controlled release of drugs on demand still represent a challenge. Recently, gas-filled microparticles, so-called antibubbles, have been developed which can efficiently encapsulate liquid drug droplets. Here, we show that antibubbles are efficiently taken up by macrophages in vitro and are stably maintained for more than 48 h without compromising antibubble integrity and macrophage viability. We show that application of diagnostic ultrasound induces the disintegration of both antibubbles and carrier cells while not affecting non-loaded macrophages. Using 4-hydroxytamoxifen as a model drug, we show ultrasound-mediated drug release upon adoptive transfer of antibubble-loaded macrophages in mice. Together with the ability of macrophages to accumulate in inflamed tissues, antibubble-loaded macrophages represent an attractive tool for targeted delivery of drugs and its ultrasound-mediated spatial and temporal drug release, highlighting the therapeutic perspective of this strategy.
Leptomeningeal metastatic disease: new frontiers and future directions
Authors: Ahmad Ozair, Hannah Wilding, Debarati Bhanja, Nicholas Mikolajewicz, Michael Glantz, Stuart A Grossman, Arjun Sahgal, Emilie Le Rhun, Michael Weller, Tobias Weiss, Tracy T Batchelor, Patrick Y Wen, Daphne A Haas-Kogan, Mustafa Khasraw, Roberta Rudà, Riccardo Soffietti, Philipp Vollmuth, Vivek Subbiah, Chetan Bettegowda, Lily C Pham, Graeme F Woodworth, Manmeet S Ahluwalia, Alireza Mansouri
Journal: Nature Reviews Clinical Oncology (2024)
One term to transform: universal health coverage through professional community health workers
Authors: Madeleine Ballard, Bernice Dahn, James O'Donovan, Ariwame Jiménez, Patrick Kawooya, Mallika Raghavan, Niloofar Ganjian, Ari D Johnson, Chisomo Boxer, Katelin Gray, Daniel Palazuelos, Christina Berry-Moorcroft, Zeus Aranda, M Matías Iberico, Laura Cordier, Dickson Mbewe, Erick Kiprotich Yegon, Josef Ernst, Lydia DiStefano, Tom Traill, Karen E Finnegan, Luc Rakotonirina, Rachel Hofmann, Ellen D Sano, Jamie Sewan Johnston, Victoria Ward, Carey Westgate, Theebika Shanmugarasa, Rebecca Alban, Dillon Mann, Rindang Asmara-Petersen, Pauline Keronyai, Dykki Settle, Jude Aidam, Albert Obbuyi, Olusola Oladeji, Prossy Muyingo, Kathleen Cho, Maryse Kok
Journal: The Lancet (2024)
Regional action needed to halt antimalarial drug resistance in Africa
Authors: Rosario Martinez-Vega, Deus S Ishengoma, Roly Gosling, Philip J Rosenthal, Arjen Dondorp, Karen I Barnes, Christian Nsanzabana, Abdoulaye A Djimde, Lynette I Ochola-Oyier, James Tibenderana, John Chimumbwa, Lemu Golassa, Ntuli A Kapologwe, Wilfred F Mbacham, Moses R Kamya, David A Fidock, Ryuichi Komatsu, Lorenz von Seidlein, Mehul Dhorda
Journal: The Lancet (2024)
Bisphosphonate-mineralized nano-IFNγ suppresses residual tumor growth caused by incomplete radiofrequency ablation through metabolically remodeling tumor-associated macrophages
Authors: Zhicheng Yan, Bing Wang, Yuhan Shen, Junji Ren, Meifang Chen, Yunhui Jiang, Hao Wu, Wenbing Dai, Hua Zhang, Xueqing Wang, Qiang Zhang, Wei Yang, Bing He
Journal: Theranostics (2024)

Rationale: Radiofrequency ablation (RFA), as a minimally invasive surgery strategy based on local thermal-killing effect, is widely used in the clinical treatment of multiple solid tumors. Nevertheless, RFA cannot achieve the complete elimination of tumor lesions with larger burden or proximity to blood vessels. Incomplete RFA (iRFA) has even been validated to promote residual tumor growth due to the suppressive tumor immune microenvironment (TIME). Therefore, exploring strategies to remodel TIME is a key issue for the development of RFA therapy. Methods: The negative effect of iRFA on colorectal cancer therapy was firstly investigated. Then a zoledronate-mineralized nanoparticle loaded with IFNγ (Nano-IFNγ/Zole) was designed and its tumor suppressive efficacy was evaluated. Finally, the metabolic reprogramming mechanism of Nano-IFNγ/Zole on tumor-associated macrophages (TAMs) was studied in detail. Results: We found iRFA dynamically altered TIME and promoted TAM differentiation from M1 to M2. Nano-IFNγ/Zole was fabricated to metabolically remodel TAMs. IFNγ in Nano-IFNγ/Zole concentrated in the ablation site to play a long-term remodeling role. Acting on mevalonate pathway, Nano-IFNγ/Zole was discovered to reduce lysosomal acidification and activate transcription factor TFEB by inhibiting isoprene modification of the Rab protein family. These mechanisms, in conjunction with IFNγ-activated JAK/STAT1 signaling, accelerated the reprogramming of TAMs from M2 to M1, and suppressed tumor recurrence after iRFA. Conclusions: This study elaborates the synergistic mechanism of zoledronate and IFNγ in Nano-IFNγ/Zole to reshape suppressive TIME caused by iRFA by remodeling TAMs, and highlights the important value of metabolically induced cellular reprogramming. Since both zoledronate and IFNγ have already been approved in clinics, this integrative nano-drug delivery system establishes an effective strategy with great translational promise to overcome the poor prognosis after clinically incomplete RFA.
Non-invasive volumetric ultrasound localization microscopy detects vascular changes in mice with Alzheimer's disease
Authors: Rebecca M Jones, Ryan M DeRuiter, Mohanish Deshmukh, Paul A Dayton, Gianmarco F Pinton
Journal: Theranostics (2024)

Alzheimer's Disease (AD) is the most common form of dementia and one of the leading causes of death. AD is known to be correlated to tortuosity in the microvasculature as well as decreases in blood flow throughout the brain. However, the mechanisms behind these changes and their causal relation to AD are poorly understood.
Drug repositioning of mesalamine via supramolecular nanoassembly for the treatment of drug-induced acute liver failure
Authors: Byeongmin Park, Eun Hye Kim, Hochung Jang, Yelee Kim, Youngri Ryu, Jiwoong Choi, Dongwon Shin, Myung Chul Lee, Yoosoo Yang, Kwangmeyung Kim, Sangmin Lee, Sun Hwa Kim, Man Kyu Shim
Journal: Theranostics (2024)
Advances in subpopulation separation and detection of extracellular vesicles: for liquid biopsy and downstream research
Authors: Zi-Xiu Liu, Gang Chen, Zi-Li Yu
Journal: Theranostics (2024)

Extracellular vesicles (EVs) are carriers of a diverse array of bioactive molecules, making them valuable clinical tools for liquid biopsy in disease diagnosis and prognosis evaluation. These molecules play critical roles in various physiological and pathological conditions, and effective separation of EVs is essential to achieve these objectives. Due to the high heterogeneity of EVs, particularly with regard to their cargo molecules, merely isolating the general EV population is inadequate for liquid biopsy and biological function studies. Therefore, separating EV subpopulations becomes crucial. Traditional separation methods, such as differential ultracentrifugation and size exclusion chromatography, along with burgeoning techniques like classical microfluidic chips and covalent chemistry, often prove time-consuming, yield low purity, and have limited ability to address cargo heterogeneity. Thus, precise separation of EV subpopulations is of utmost importance. Additionally, detecting subpopulation-specific cargo is vital for validating the effectiveness of separation methods and supporting clinical biopsy applications. However, reviews that focus specifically on detection methods for EV subpopulations are limited. This paper provides a comprehensive overview of the methods for separating and detecting EV subpopulations with surface marker heterogeneity, comparing the advantages and limitations of each technique. Furthermore, it discusses challenges and future prospects for these methods in the context of liquid biopsy and downstream research. Collectively, this review aims to offer innovative insights into the separation and detection of EV subpopulations, guiding researchers to avoid common pitfalls and refine their investigative approaches.
Optical molecular imaging technology and its application in precise surgical navigation of liver cancer
Authors: Pan He, Haitian Tang, Yating Zheng, Xiao Xu, Xuqi Peng, Tao Jiang, Yongfu Xiong, Yang Zhang, Yu Zhang, Gang Liu
Journal: Theranostics (2024)

Recent innovations in medical imaging technology have placed molecular imaging techniques at the forefront of diagnostic advancements. The current research trajectory in this field aims to integrate personalized molecular data of patients and diseases with traditional anatomical imaging data, enabling more precise, non-invasive, or minimally invasive diagnostic options for clinical medicine. This article provides an in-depth exploration of the basic principles and system components of optical molecular imaging technology. It also examines commonly used targeting mechanisms of optical probes, focusing especially on indocyanine green-the FDA-approved optical dye widely used in clinical settings-and its specific applications in diagnosing and treating liver cancer. Finally, this review highlights the advantages, limitations, and future challenges facing optical molecular imaging technology, offering a comprehensive overview of recent advances, clinical applications, and potential impacts on liver cancer treatment strategies.
Ultra-small quercetin-based nanotherapeutics ameliorate acute liver failure by combatting inflammation/cellular senescence cycle
Authors: Yali Feng, Xiaoli Zhang, Juan Li, Shan Fu, Weicheng Xu, Jinfeng Liu, Yuan Yang, Tianyan Chen, Yingren Zhao, Dongmin Li, Mingzhen Zhang, Yingli He
Journal: Theranostics (2024)
Melt Electrowriting of Elastic Scaffolds Using PEOT‐PBT Multi‐block Copolymer
Authors: Armin Amirsadeghi, Pavan Kumar Reddy Gudeti, Sietse Tock, Marcus Koch, Daniele Parisi, Marleen Kamperman, Małgorzata Katarzyna Włodarczyk‐Biegun
Journal: Advanced Healthcare Materials (2024)

Melt electrowriting (MEW) is a powerful additive manufacturing technique to produce tissue engineering scaffolds. Despite its strength, it is limited by a small number of processable polymers. Therefore, to broaden the library of materials for MEW, we investigated the printability of poly(ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT-PBT), a thermoplastic elastomer. The effect of different printing parameters and material thermal degradation are studied. It is observed that the material is stable for >60 min at a printing temperature of 195 °C in a nitrogen environment. Next, two types of designs are printed and characterized: mesh-like and semi-random scaffolds. For both types of designs, PEOT-PBT scaffolds reveal a higher yield strain, and lower Young's modulus as compared to control polycaprolactone scaffolds. Biological studies performed using mouse embryonic fibroblasts (NIH-3T3) show good cell viability and metabolic activity on all print scaffolds. SEM imaging reveals actively migrating cells on PEOT-PBT mesh scaffolds after 24 h of culture and 98.87% of pore bridging by cells after 28 days of culture. Immunofluorescence staining shows decreased expression of alpha-smooth muscle actin from day 14 to day 28 in PEOT-PBT mesh scaffolds. Overall, it is shown that melt electrowritten PEOT-PBT scaffolds have great potential for soft tissue regeneration.
Curvature Perception of Mesenchymal Cells on Mesoscale Topographies
Authors: Kerstin Frey, Michael Brunner, Christóbal Curio, Ralf Kemkemer
Journal: Advanced Healthcare Materials (2024)

Cells can sense geometrical cues with sizes of several tens of micrometers in their vicinity. Recent in vitro studies show that cells can adapt their shape, align along specific directions, or regulate other cellular functions when grown on surfaces with curvatures larger than their size. Although possible mechanisms for such responses like the alignment along axial cues have been suggested, a detailed understanding of the involved cellular processes remains open. This work addresses this gap by systematically investigating mesenchymal cell and nucleus orientation responses using a low-cost model surface platform, the CurvChip. Using an array of cylindrically curved topographies with radii of curvatures ranging from tens to hundreds of micrometers, the contact guidance response of cells and nuclei is quantified in dependence on substratum curvature and manipulation of cytoskeletal components. Results suggest a desired perceived curvature for the investigated cells, and a very sensitive and robust curvature perception mechanism, as the effect of pharmacological manipulation of cytoskeletal components is relatively small. Furthermore, a comparison with previously published work strengthens the hypothesis of an involvement of the nucleus in the cell response to three-dimensional (3D) curvatures.
Efficacy of Midostaurin Combined With Intensive Chemotherapy in Core Binding Factor Leukemia: A Phase <scp>II</scp> Clinical Trial
Authors: Roberto Cairoli, Arianna Gatti, Giovanni Grillo, Marta Rachele Stefanucci, Barbara Di Camillo, Monica Fumagalli, Mauro Krampera, Gianpaolo Nadali, Patrizia Zappasodi, Erika Borlenghi, Elisabetta Todisco, Marta Ubezio, Massimo Bernardi, Alfredo Molteni, Claudia Basilico, Mauro Turrini, Rosa Greco, Valentina Mancini, Marta Riva, Davide Paolo Bernasconi, Bruno Brando, Silvio Marco Veronese, Alessandro Beghini
Journal: American Journal of Hematology (2024)

Samples from 34 adult patients newly diagnosed with core binding factor leukemia (CBFL) were collected both at the time of diagnosis and at relapse and were centrally analyzed. Eligible patients received either standard induction CT known as "3 + 7" or an equivalent regimen, according to the recruiting center's policy. Patients who achieved CR or CRi received 3 courses of high-dose ARA-C (Cytarabine) 3000 mg/m
The Impact of Xanomeline and Trospium Chloride on Cognitive Impairment in Acute Schizophrenia: Replication in Pooled Data From Two Phase 3 Trials
Authors: William P. Horan, Colin Sauder, Philip D. Harvey, Ian S. Ramsay, Samantha E. Yohn, Richard S. E. Keefe, Vicki G. Davis, Steven M. Paul, Stephen K. Brannan
Journal: American Journal of Psychiatry (2024)

Xanomeline and trospium chloride (formerly known as KarXT), a novel M
Current Advances in Behavioral Addictions: From Fundamental Research to Clinical Practice
Authors: Matthias Brand, Stephanie Antons, Beata Bőthe, Zsolt Demetrovics, Naomi A. Fineberg, Susana Jimenez-Murcia, Daniel L. King, Gemma Mestre-Bach, Tania Moretta, Astrid Müller, Elisa Wegmann, Marc N. Potenza
Journal: American Journal of Psychiatry (2024)

Gambling disorder is the only behavioral addiction recognized as a clinical disorder in DSM-5, and Internet gaming disorder is included as a condition requiring further research. ICD-11 categorizes gambling and gaming disorders as disorders due to addictive behaviors. Additional behavioral addictions may include compulsive sexual behavior disorder, compulsive buying-shopping disorder, and problematic use of social media. This narrative review summarizes the current state of knowledge regarding these five (potential) disorders due to addictive behaviors. All five (potential) disorders are clinically relevant and prevalent. Behavioral addictions frequently co-occur with other mental and behavioral problems, such as depression, anxiety, and attention deficit hyperactivity disorder. Validated diagnostic instruments exist, with empirical support varying across conditions. No medications have approved indications from regulatory bodies for behavioral addictions, and cognitive-behavioral therapy has the most empirical support for efficacious treatment. Given that behavioral addictions are prevalent, frequently co-occur with psychiatric disorders, may often go undiagnosed and untreated, and have been linked to poorer treatment outcomes, active screening and treatment are indicated. Public health considerations should be expanded, and impacts of modern technologies should be investigated more intensively. Treatment optimization involving pharmacotherapy, psychotherapy, neuromodulation, and their combination warrants additional investigation.