tbuckley/cabot-search huggingface dataset:
Advances in Microfluidic‐Based Core@Shell Nanoparticles Fabrication for Cancer Applications
Authors: Duarte R. S. Almeida, João Ferreira Gil, Antonio José Guillot, Jiachen Li, Ricardo J. B. Pinto, Hélder A. Santos, Gil Gonçalves
Journal: Advanced Healthcare Materials (2024)

Current research in cancer therapy focuses on personalized therapies, through nanotechnology-based targeted drug delivery systems. Particularly, controlled drug release with nanoparticles (NPs) can be designed to safely transport various active agents, optimizing delivery to specific organs and tumors, minimizing side effects. The use of microfluidics (MFs) in this field has stood out against conventional methods by allowing precise control over parameters like size, structure, composition, and mechanical/biological properties of nanoscale carriers. This review compiles applications of microfluidics in the production of core-shell NPs (CSNPs) for cancer therapy, discussing the versatility inherent in various microchannel and/or micromixer setups and showcasing how these setups can be utilized individually or in combination, as well as how this technology allows the development of new advances in more efficient and controlled fabrication of core-shell nanoformulations. Recent biological studies have achieved an effective, safe, and controlled delivery of otherwise unreliable encapsulants such as small interfering RNA (siRNA), plasmid DNA (pDNA), and cisplatin as a result of precisely tuned fabrication of nanocarriers, showing that this technology is paving the way for innovative strategies in cancer therapy nanofabrication, characterized by continuous production and high reproducibility. Finally, this review analyzes the technical, biological, and technological limitations that currently prevent this technology from becoming the standard.
Integrated proteogenomic analysis for inherited bone marrow failure syndrome
Authors: Manabu Wakamatsu, Hideki Muramatsu, Hironori Sato, Masaki Ishikawa, Ryo Konno, Daisuke Nakajima, Motoharu Hamada, Yusuke Okuno, Yusuke Kawashima, Asahito Hama, Masafumi Ito, Hideto Iwafuchi, Yoshiyuki Takahashi, Osamu Ohara
Journal: Leukemia (2024)

Abstract Recent advances in in-depth data-independent acquisition proteomic analysis have enabled comprehensive quantitative analysis of &gt;10,000 proteins. Herein, an integrated proteogenomic analysis for inherited bone marrow failure syndrome (IBMFS) was performed to reveal their biological features and to develop a proteomic-based diagnostic assay in the discovery cohort; dyskeratosis congenita ( n = 12), Fanconi anemia ( n = 11), Diamond–Blackfan anemia (DBA, n = 9), Shwachman–Diamond syndrome (SDS, n = 6), ADH5/ALDH2 deficiency ( n = 4), and other IBMFS ( n = 18). Unsupervised proteomic clustering identified eight independent clusters (C1–C8), with the ribosomal pathway specifically downregulated in C1 and C2, enriched for DBA and SDS, respectively. Six patients with SDS had significantly decreased SBDS protein expression, with two of these not diagnosed by DNA sequencing alone. Four patients with ADH5/ALDH2 deficiency showed significantly reduced ADH5 protein expression. To perform a large-scale rapid IBMFS screening, targeted proteomic analysis was performed on 417 samples from patients with IBMFS-related hematological disorders ( n = 390) and healthy controls ( n = 27). SBDS and ADH5 protein expressions were significantly reduced in SDS and ADH5/ALDH2 deficiency, respectively. The clinical application of this first integrated proteogenomic analysis would be useful for the diagnosis and screening of IBMFS, where appropriate clinical screening tests are lacking.
Biomaterials science and surface engineering strategies for dental peri-implantitis management
Authors: Ya-Meng Yu, Yu-Pu Lu, Ting Zhang, Yu-Feng Zheng, Yun-Song Liu, Dan-Dan Xia
Journal: Military Medical Research (2024)

Abstract Peri-implantitis is a bacterial infection that causes soft tissue inflammatory lesions and alveolar bone resorption, ultimately resulting in implant failure. Dental implants for clinical use barely have antibacterial properties, and bacterial colonization and biofilm formation on the dental implants are major causes of peri-implantitis. Treatment strategies such as mechanical debridement and antibiotic therapy have been used to remove dental plaque. However, it is particularly important to prevent the occurrence of peri-implantitis rather than treatment. Therefore, the current research spot has focused on improving the antibacterial properties of dental implants, such as the construction of specific micro-nano surface texture, the introduction of diverse functional coatings, or the application of materials with intrinsic antibacterial properties. The aforementioned antibacterial surfaces can be incorporated with bioactive molecules, metallic nanoparticles, or other functional components to further enhance the osteogenic properties and accelerate the healing process. In this review, we summarize the recent developments in biomaterial science and the modification strategies applied to dental implants to inhibit biofilm formation and facilitate bone-implant integration. Furthermore, we summarized the obstacles existing in the process of laboratory research to reach the clinic products, and propose corresponding directions for future developments and research perspectives, so that to provide insights into the rational design and construction of dental implants with the aim to balance antibacterial efficacy, biological safety, and osteogenic property.
A Male Patient with Hydrocephalus via Multimodality Diagnostic Approaches: A Case Report
Authors: Xiuyun Liu, Jingjing Mu, Meijun Pang, Xuehai Fan, Ziwei Zhou, Fang Guo, Kai Yu, Huijie Yu, Dong Ming
Journal: Cyborg and Bionic Systems (2024)

Introduction: Idiopathic normal pressure hydrocephalus (iNPH) is a kind of hydrocephalus that is easily to be misdiagnosed with brain atrophy due to the similarity of ventricular dilation and cognitive impairment. In this case, we present an old male patient who was diagnosed with iNPH by multimodality approaches. Outcomes: A 68-year-old male patient, with deteriorated gait, declined cognitive function for at least 3 years and urinary incontinence for 3 months. The doctors suspected him a patient with hydrocephalus or Alzheimer's disease based on his symptoms. We used multimodality diagnostic approaches including brain imaging, cerebrospinal fluid tap test, continuous intracranial pressure monitoring, and infusion study to make the final diagnosis of iNPH. He underwent ventriculoperitoneal shunt surgery and was well recovered. Conclusion: This case demonstrates the efficacy of using multimodality approaches for iNPH diagnosis, which saves patient time and clinical cost, worthy of further promotion.
Locomotion control of cyborg insects by charge-balanced biphasic electrical stimulation
Authors: Zhong Liu, Yongxia Gu, Li Yu, Xiang Yang, Zhiyun Ma, Jieliang Zhao, Yufei Gu
Journal: Cyborg and Bionic Systems (2024)

The integration of electronic stimulation devices with insects in the context of cyborg insect systems has great application potential, particularly in the fields of environmental monitoring, urban surveillance, and rescue missions. Despite considerable advantages compared to the current robot technology, including flexibility, durability, and low energy consumption, this integration faces certain challenges related to the potential risk of charge accumulation caused by prolonged and repetitive electrical stimulations. To address these challenges, this study proposes a universal system for remote signal output control using infrared signals. The proposed system integrates high-precision digital-to-analog converters capable of generating customized waveform electrical stimulation signals within defined ranges. This enhances the accuracy of locomotion control in cyborg insects while maintaining real-time control and dynamic parameter adjustment. The proposed system is verified by experiments. The experimental results show that the signals generated by the proposed system have a success rate of over 76.25% in controlling the turning locomotion of cyborg insects, which is higher than previously reported results. In addition, the charge-balanced characteristics of these signals can minimize muscle tissue damage, thus substantially enhancing control repeatability. This study provides a comprehensive solution for the remote control and monitoring of cyborg insects, whose flexibility and adaptability can meet various application and experimental requirements. The results presented in this study lay a robust foundation for further advancement of various technologies, particularly those related to cyborg insect locomotion control systems and wireless control mechanisms for cyborg insects.
A novel approach to exploring the dark genome and its application to mapping of the vertebrate virus fossil record
Authors: Daniel Blanco-Melo, Matthew A Campbell, Henan Zhu, Tristan P W Dennis, Sejal Modha, Spyros Lytras, Joseph Hughes, Anna Gatseva, Robert J Gifford
Journal: Genome biology (2024)

Abstract Background Genomic regions that remain poorly understood, often referred to as the dark genome, contain a variety of functionally relevant and biologically informative features. These include endogenous viral elements (EVEs)—virus-derived sequences that can dramatically impact host biology and serve as a virus fossil record. In this study, we introduce a database-integrated genome screening (DIGS) approach to investigate the dark genome in silico, focusing on EVEs found within vertebrate genomes. Results Using DIGS on 874 vertebrate genomes, we uncover approximately 1.1 million EVE sequences, with over 99% originating from endogenous retroviruses or transposable elements that contain EVE DNA. We show that the remaining 6038 sequences represent over a thousand distinct horizontal gene transfer events across 10 virus families, including some that have not previously been reported as EVEs. We explore the genomic and phylogenetic characteristics of non-retroviral EVEs and determine their rates of acquisition during vertebrate evolution. Our study uncovers novel virus diversity, broadens knowledge of virus distribution among vertebrate hosts, and provides new insights into the ecology and evolution of vertebrate viruses. Conclusions We comprehensively catalog and analyze EVEs within 874 vertebrate genomes, shedding light on the distribution, diversity, and long-term evolution of viruses and reveal their extensive impact on vertebrate genome evolution. Our results demonstrate the power of linking a relational database management system to a similarity search-based screening pipeline for in silico exploration of the dark genome.
SCIPAC: quantitative estimation of cell-phenotype associations
Authors: Dailin Gan, Yini Zhu, Xin Lu, Jun Li
Journal: Genome biology (2024)

Abstract Numerous algorithms have been proposed to identify cell types in single-cell RNA sequencing data, yet a fundamental problem remains: determining associations between cells and phenotypes such as cancer. We develop SCIPAC, the first algorithm that quantitatively estimates the association between each cell in single-cell data and a phenotype. SCIPAC also provides a p -value for each association and applies to data with virtually any type of phenotype. We demonstrate SCIPAC’s accuracy in simulated data. On four real cancerous or noncancerous datasets, insights from SCIPAC help interpret the data and generate new hypotheses. SCIPAC requires minimum tuning and is computationally very fast.
Toolbox of individual-level interventions against online misinformation
Authors: Anastasia Kozyreva, Philipp Lorenz-Spreen, Stefan M Herzog, Ullrich K H Ecker, Stephan Lewandowsky, Ralph Hertwig, Ayesha Ali, Joe Bak-Coleman, Sarit Barzilai, Melisa Basol, Adam J Berinsky, Cornelia Betsch, John Cook, Lisa K Fazio, Michael Geers, Andrew M Guess, Haifeng Huang, Horacio Larreguy, Rakoen Maertens, Folco Panizza, Gordon Pennycook, David G Rand, Steve Rathje, Jason Reifler, Philipp Schmid, Mark Smith, Briony Swire-Thompson, Paula Szewach, Sander van der Linden, Sam Wineburg
Journal: Nature Human Behaviour (2024)
Single‐cell profiling highlight pathogenic myeloid features in house dust mite‐induced murine model of airway inflammation
Authors: Jiachen Liu, Jiaoyan Lv, Sijie Chen, Jie Ma, Jiaying Yao, Zan Yuan, Chunyuan Yang, Xiao Li, Xuegong Zhang, Yunping Zhu, Jianhong Zhang, Li Wu
Journal: Allergy (2024)

The data that support the findings of this study are openly available in the Genome Sequence Archive (GSA) at https://ngdc.cncb.ac.cn/gsa/s/E6877KDn, reference number CRA013812. Data S1: Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Human dental pulp stem cells mitigate the neuropathology and cognitive decline via AKT-GSK3β-Nrf2 pathways in Alzheimer’s disease
Authors: Wei Xiong, Ye Liu, Heng Zhou, Junyi Li, Shuili Jing, Cailei Jiang, Mei Li, Yan He, Qingsong Ye
Journal: International Journal of Oral Science (2024)

Abstract Oxidative stress is increasingly recognized as a major contributor to the pathophysiology of Alzheimer’s disease (AD), particularly in the early stages of the disease. The multiplicity advantages of stem cell transplantation make it fascinating therapeutic strategy for many neurodegenerative diseases. We herein demonstrated that human dental pulp stem cells (hDPSCs) mediated oxidative stress improvement and neuroreparative effects in in vitro AD models, playing critical roles in regulating the polarization of hyperreactive microglia cells and the recovery of damaged neurons. Importantly, these therapeutic effects were reflected in 10-month-old 3xTg-AD mice after a single transplantation of hDPSCs, with the treated mice showing significant improvement in cognitive function and neuropathological features. Mechanistically, antioxidant and neuroprotective effects, as well as cognitive enhancements elicited by hDPSCs, were at least partially mediated by Nrf2 nuclear accumulation and downstream antioxidant enzymes expression through the activation of the AKT-GSK3β-Nrf2 signaling pathway. In conclusion, our findings corroborated the neuroprotective capacity of hDPSCs to reshape the neuropathological microenvironment in both in vitro and in vivo AD models, which may be a tremendous potential therapeutic candidate for Alzheimer’s disease.
Harnessing clonal gametes in hybrid crops to engineer polyploid genomes
Authors: Yazhong Wang, Roven Rommel Fuentes, Willem M. J. van Rengs, Sieglinde Effgen, Mohd Waznul Adly Mohd Zaidan, Rainer Franzen, Tamara Susanto, Joiselle Blanche Fernandes, Raphael Mercier, Charles J. Underwood
Journal: Nature Genetics (2024)

Abstract Heterosis boosts crop yield; however, harnessing additional progressive heterosis in polyploids is challenging for breeders. We bioengineered a ‘mitosis instead of meiosis’ ( MiMe ) system that generates unreduced, clonal gametes in three hybrid tomato genotypes and used it to establish polyploid genome design. Through the hybridization of MiMe hybrids, we generated ‘4-haplotype’ plants that encompassed the complete genetics of their four inbred grandparents, providing a blueprint for exploiting polyploidy in crops.
Nanozymes for Treating Ocular Diseases
Authors: Ka‐Ying Wong, Man‐Sau Wong, Juewen Liu
Journal: Advanced Healthcare Materials (2024)

Nanozymes, characterized by their nanoscale size and enzyme-like catalytic activities, exhibit diverse therapeutic potentials, including anti-oxidative, anti-inflammatory, anti-microbial, and anti-angiogenic effects. These properties make them highly valuable in nanomedicine, particularly ocular therapy, bypassing the need for systemic delivery. Nanozymes show significant promise in tackling multi-factored ocular diseases, particularly those influenced by oxidation and inflammation, like dry eye disease, and age-related macular degeneration. Their small size, coupled with their ease of modification and integration into soft materials, facilitates the effective penetration of ocular barriers, thereby enabling targeted or prolonged therapy within the eye. This review is dedicated to exploring ocular diseases that are intricately linked to oxidation and inflammation, shedding light on the role of nanozymes in managing these conditions. Additionally, recent studies elucidating advanced applications of nanozymes in ocular therapeutics, along with their integration with soft materials for disease management, are discussed. Finally, this review outlines directions for future investigations aimed at bridging the gap between nanozyme research and clinical applications.
Targeting TGFβ-activated kinase-1 activation in microglia reduces CAR T immune effector cell-associated neurotoxicity syndrome
Authors: Janaki Manoja Vinnakota, Francesca Biavasco, Marius Schwabenland, Chintan Chhatbar, Rachael C. Adams, Daniel Erny, Sandra Duquesne, Nadia El Khawanky, Dominik Schmidt, Viktor Fetsch, Alexander Zähringer, Henrike Salié, Dimitrios Athanassopoulos, Lukas M. Braun, Nora R. Javorniczky, Jenny N. H. G. Ho, Katrin Kierdorf, Reinhard Marks, Ralph Wäsch, Federico Simonetta, Geoffroy Andrieux, Dietmar Pfeifer, Gianni Monaco, Christian Capitini, Terry J. Fry, Thomas Blank, Bruce R. Blazar, Eva Wagner, Matthias Theobald, Clemens Sommer, Matthias Stelljes, Christian Reicherts, Astrid Jeibmann, Jens Schittenhelm, Camelia-Maria Monoranu, Andreas Rosenwald, Martin Kortüm, Leo Rasche, Hermann Einsele, Philipp T. Meyer, Joachim Brumberg, Simon Völkl, Andreas Mackensen, Roland Coras, Michael von Bergwelt-Baildon, Nathalie L. Albert, Laura M. Bartos, Matthias Brendel, Adrien Holzgreve, Matthias Mack, Melanie Boerries, Crystal L. Mackall, Justus Duyster, Philipp Henneke, Josef Priller, Natalie Köhler, Felix Strübing, Bertram Bengsch, Marco Ruella, Marion Subklewe, Louisa von Baumgarten, Saar Gill, Marco Prinz, Robert Zeiser
Journal: Nature Cancer (2024)
Insulin resistance and psoriasis
Authors: Shirley P Parraga, Steven R Feldman
Journal: British Journal of Dermatology (2024)

Journal Article Accepted manuscript Insulin resistance and psoriasis Get access Shirley P Parraga, Shirley P Parraga Wake Forest University School of Medicine, Winston-Salem, NC, USA https://orcid.org/0009-0002-1835-1311 Search for other works by this author on: Oxford Academic Google Scholar Steven R Feldman Steven R Feldman Wake Forest University School of Medicine, Winston-Salem, NC, USACenter for Dermatology Research, Department of Dermatology, Winston-Salem, NC, USADepartment of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC, USADepartment of Social Sciences & Health Policy, Wake Forest University School of Medicine, Winston-Salem, NC, USA Corresponding author: Steven R. Feldman, Email:sfeldman@wakehealth.edu https://orcid.org/0000-0002-0090-6289 Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, ljae199, https://doi.org/10.1093/bjd/ljae199 Published: 13 May 2024 Article history Received: 30 April 2024 Accepted: 09 May 2024 Published: 13 May 2024
Anti‐Stress and Anti‐ROS Effects of MnOx‐Functionalized Thermosensitive Nanohydrogel Protect BMSCs for Intervertebral Disc Degeneration Repair
Authors: Xiaoxiong Wang, Liyang Yu, Jiazhi Duan, Mingzheng Chang, Min Hao, Ziqian Xiang, Cheng Qiu, Junyuan Sun, Derun Di, He Xia, Dezheng Li, Suomao Yuan, Yonghao Tian, Jichuan Qiu, Hong Liu, Xinyu Liu, Yuanhua Sang, Lianlei Wang
Journal: Advanced Healthcare Materials (2024)

Stem cell transplantation is proven to be a promising strategy for intervertebral disc degeneration (IDD) repair. However, replicative senescence of bone marrow-derived mesenchymal stem cells (BMSCs), shear damage during direct injection, mechanical stress, and the reactive oxygen species (ROS)-rich microenvironment in degenerative intervertebral discs (IVDs) cause significant cellular damage and limit the therapeutic efficacy. Here, an injectable manganese oxide (MnOx)-functionalized thermosensitive nanohydrogel is proposed for BMSC transplantation for IDD therapy. The MnOx-functionalized thermosensitive nanohydrogel not only successfully protects BMSCs from shear force and mechanical stress before and after injection, but also repairs the harsh high-ROS environment in degenerative IVDs, thus effectively increasing the viability of BMSCs and resident nucleus pulposus cells (NPCs). The MnOx-functionalized thermosensitive nanohydrogel provides mechanical protection for stem cells and helps to remove endogenous ROS, providing a promising stem cell delivery platform for the treatment of IDD.
Bursting the bubble on diagnostics: Artificial intelligence in autoimmune bullous disease
Authors: Brent J Doolan, Bjorn R Thomas
Journal: British Journal of Dermatology (2024)

Journal Article Accepted manuscript Bursting the bubble on diagnostics: Artificial intelligence in autoimmune bullous disease Get access Brent J Doolan, Brent J Doolan St John's Institute of Dermatology, School of Basic and Medical Biosciences, King's College London, London, UKSt John's Institute of Dermatology, Guy's and St Thomas' Hospital, London, UK Corresponding Author: Dr Bjorn Thomas, Email:Bjorn.thomas@gstt.nhs.uk https://orcid.org/0000-0002-9497-0504 Search for other works by this author on: Oxford Academic Google Scholar Bjorn R Thomas Bjorn R Thomas St John's Institute of Dermatology, Guy's and St Thomas' Hospital, London, UK https://orcid.org/0000-0002-1815-3502 Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, ljae197, https://doi.org/10.1093/bjd/ljae197 Published: 13 May 2024 Article history Received: 28 April 2024 Accepted: 09 May 2024 Published: 13 May 2024
Vitamin D status in patients with erythropoietic protoporphyria taking the systemic photoprotective agent afamelanotide
Authors: Lesley E Rhodes
Journal: British Journal of Dermatology (2024)

Journal Article Accepted manuscript Vitamin D status in EPP patients taking the systemic photoprotective agent afamelanotide Get access Lesley E Rhodes Lesley E Rhodes Dermatology Centre, School of Biological Sciences, Faculty of Biology Medicine & Health, The University of Manchester, Manchester, UKSalford Royal Hospital, Northern Care Alliance NHS Trust, Greater Manchester, UK Corresponding author: Email:lesley.e.rhodes@manchester.ac.uk https://orcid.org/0000-0002-9107-6654 Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, ljae191, https://doi.org/10.1093/bjd/ljae191 Published: 13 May 2024 Article history Received: 01 May 2024 Accepted: 09 May 2024 Published: 13 May 2024
Secukinumab in adult patients with lichen planus: efficacy and safety results from the randomized placebo-controlled proof-of-concept PRELUDE study
Authors: Thierry Passeron, Maximilian Reinhardt, Benjamin Ehst, Jonathan Weiss, Jason Sluzevich, Michael Sticherling, Pascal Reygagne, Johannes Wohlrab, Michael Hertl, Nasim Fazel, Elisa Muscianisi, Heng Fan, Isabelle Hampele, Nicolò Compagno
Journal: British Journal of Dermatology (2024)

Patients with lichen planus (LP) refractory to available therapies often experience a high disease burden, representing a population with a clear unmet need for new treatments.
‘Feeling like a second-class citizen’: exploring the impact, self-management and existing support for adults living with acne and/or post-inflammatory hyperpigmentation and scarring
Authors: Carys Dale, Rachael M Hewitt, Catherine Purcell, Neesha Pandya, Chris Bundy
Journal: British Journal of Dermatology (2024)

This article investigates how living with, and self-managing, acne and post-inflammatory hyperpigmentation and scarring impacts adults living with these conditions. Eight adults took part in individual qualitative interviews. Framework analysis generated four themes: (1) acne blame; (2) control at any cost; (3) the business of skinfluencers; and (4) ‘it’s your hormones’. Those with acne and PIH&amp;S are currently underserved by dermatology and psychodermatology provision, and additional funding and research is needed to provide psychological and self-management support for this group.