tbuckley/cabot-search huggingface dataset:
Interleukin-33 Increase Contraction and Mast Cell Mediator Release in Isolated Human Small Airways
Authors: J. Säfholm, A.-K. Johnsson, J. Kolmert, M. Adner, G. Nilsson, C.E. Wheelock, S.-E. Dahlén, M. Belikova
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Machine Learning to Predict the Onset of Ventilator-associated Pneumonia Using Electronic Health Record Data
Authors: A. Peltekian, K. Senkow, N.S. Markov, M. Kang, A. Choudhary, A. Misharin, B.D. Singer, G.S. Budinger, R.G. Wunderink, A. Agrawal, C.A. Gao
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
In Children With Asthma, GSDMB Polymorphism rs7216389 Is Associated With Severe Exacerbation History, Rhinovirus Replication in Bronchial Epithelium, and Epithelial Proinflammatory Cytokine Responses to Rhinovirus
Authors: E.R. Vanderwall, C.R. Gates, L.M. Rich, M.P. White, W.T. Powell, J.S. Debley
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Cold Storage of Human Precision-cut Lung Slices Preserves Cellular and Transcriptional Identity Enabling Optimized On-demand Translational Lung Research
Authors: M.C. Melo Narvaez, F. Gölitz, E. Jain, J. Gote-Schniering, M.G. Stoleriu, W. Bertrams, B. Schmeck, Y. Ali Önder, U. Rauen, T. Wille, M. Lehmann
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Dermatologic Presentation of Pulmonary Eosinophilic Granulomatosis With Polyangiitis in a Pediatric Patient
Authors: A.Z. Magier, A.E. Miller, P.R. Spata, B.C. Burbidge, E.L. Dray, B.A. Drolet, J.A. Smith, D.J. Jackson, J.J. Schulte, V. Balasubramaniam
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Extracellular Vesicle-encapsulated MicroRNA Signatures of Cigarette Smoking and Smoking-related Harm
Authors: C. Eckhardt, T. Bloomquist, N. Joglekar, P. De Hoff, P.S. Vokonas, D. Sparrow, L.C. Laurent, J. Schwartz, A.A. Baccarelli, H. Wu
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Differences in COVID-19 Vaccination Behaviors Among Adults With Versus Without Chronic Lower Respiratory Diseases: The C4R Study
Authors: T. Wang, K. Lawin, A. Kucharska-Newton, R.T. Demmer, D. Jacobs, E.A. Regan, J.S. Lee, V. Xanthakis, A.F. Norwood, C.R. Isasi, A. Kanaya, W.S. Post, K.H. Stukovsky, M.S. Elkind, V. Howard, V.E. Ortega, V. Lopez, Y. Zhang, A.M. Fretts, P. Balte, E.C. Oelsner
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Health Services Utilization After COVID-19 Hospitalization Among People With Post-Acute Sequelae of SARS-CoV-2 Infection (PASC)
Authors: M.T. Nagle, Y. Yang, K.T. Lee, E. Caldwell, A.A. Hope, T.L. Eaton, S.E. Jolley, I.D. Peltan, B. Mukherjee, T.S. Valley, C.T.L. Hough, A.J. Admon
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Lung Cancer Screening and Factors Associated With Uptake Among People With or at Increased Vulnerability for HIV: An Analysis of MACS/WIHS Combined Cohort Study
Authors: M.E. Thornton, T. Breger, A. Edmonds, A. Visconti, S. Kassaye, I. Ofotokun, F. Palella, R. Bolan, S. Krier, T. Brown, D. Jones Weiss, G. D'Souza, M. Cohen, P. Tien, T. Taylor, K. Anastos, D. Konkle-Parker, C. Ramirez, D. Westreich, S. Cole, M. Floris-Moore, M.B. Drummond
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
AI-guided Generation and Development of HXN-1011, A Highly Potent Anti-TSLP Biparatopic Antibody
Authors: H. Ran, D. Liu, Y. Zhou, L. Dong, L. Dong, Y. Wang, K. Fei, C. Chen, X. Xu, X. Chen, C. Su, L. Tian, J. Peng, Z. Zhu
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Multi-site Analysis of Functional Gas Exchange Measures on <sup>129</sup>Xe MRI Among Healthy Volunteers
Authors: A. Mchorse, H. Qin, P.J. Niedbalski, A. Schmidt, J. Liggins, J. Leung, D.D. Sin, R.L. Eddy, A.C. Swaminathan, B. Driehuys, D. Mummy
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Exploring Small Airway Dysfunction in Pre-COPD: Insights From Parametric Response Mapping, Impulse Oscillometry, and Airway Inflammatory Profiles - A Prospective Cohort Study in an East Asian Population
Authors: Y.-F. Chen, H.-C. Chen, K.-L. Lor, H.-H. Hou, N. Chien, Y.-Y. Chen, K.-Z. Lu, Z.-C. Hung, J.-Y. Chien, H.-C. Wang, C.-J. Yu
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
E-cigarette and Vaping Practices and Patterns Among U.S. Millennial Adults: A Preliminary Report From the American Lung Association Lung Health Cohort Study
Authors: R. Wharton, E. Sugar, T.-H.T. Vu, N. Mathis, S. Iyer, J.G. Wang, M. Carnethon, G.R. Washko, R. Kalhan, S. Bose
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Assessing Post-Transplant Lung Function Alterations Using Free-Breathing Hyperpolarized Xenon MRI
Authors: M.K. Ismail, H. Hamedani, L. Loza, J. Chen, M. Gorora, J. Park, E. Cantu, C. Bermudez, M.M. Crespo, A. Courtwright, A. Courtwright, J.M. Diamond, P. Gregorio, A. Gurevich, S. Kadlecek, K. Ruppert, R.R. Rizi
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Deciphering C/EBPα'S Role in Fate Plasticity in Murine and Patient Tissue Derived AT2 Cells
Authors: J. Cai, A.S. Sawhney, B. Deskin, D. Gibbard, G. Ali, A. Olson, R. Warren, S. De Langhe, Z. Inde, K. Sarosiek, E. Lemire, A. Haber, Z. Wei, A.O. Bungum, A.J. Schrandt, D.G. Brownfield
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Quantitative CT Measures of Electronic Nicotine Delivery System Users: Investigating Structural Changes and Function
Authors: J. Schroeder, M.J. Mcintosh, A.S. Kizhakke Puliyakote, E. Bruening, J.C. Sieren, A.P. Comellas, E.A. Hoffman, S.B. Fain
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
The Age-dependent Upper Limit of Normal for Hyperpolarized <sup>129</sup>Xe MRI Ventilation Defect Percent in Healthy Individuals Using a Multi-center Database
Authors: A.S. Bdaiwi, M.J. Mcintosh, R. Hussain, M. Willmering, S. Svenningsen, J.H. Rayment, G. Parraga, G. Santyr, J. Wild, R.P. Thomen, J. Mata, P.J. Niedbalski, D. Mummy, B. Driehuys, L. Bell, C. Laustsen, Z.I. Cleveland, L. Walkup, J.C. Woods, S.B. Fain, R. Eddy
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
A Unique Case of Endobronchial Cancer and Tracheobronchial Amyloidosis
Authors: L.Y.W. Shong, G.-W. Zhou, R. Tong, L. Zhao, Z.-P. Fan, Z.-W. Zheng, J. Li, C.L.D. Lam, G. Hou
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Dyspnea and Nonresolving Cough Without B-type Symptoms Leading to a Diagnosis of Fetal Adenocarcinoma of the Lung (FLAC) in Absence of Neuroendocrine Markers
Authors: Z. Yousaf, S. Shrestha, A.R. Sullivan, R. Reddy, A. Platt
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Measurement of Airway Resistance to Identify Individuals at Risk of COPD in a Population-based Cohort Study - The Hamburg City Health Study (HCHS)
Authors: B. Waschki, D. Löffers, E. Schneider, A. Haack, M. Abdo, R. Twerenbold, S. Blankenberg, E. Petersen, K.F. Rabe, C. Magnussen
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Subclinical Small-airway Abnormalities in Healthy Volunteers With High Type-2 Inflammation Biomarkers
Authors: H. Xu, N.M.J. Smith, A. Alamoudi, L. Petralia, D. Sandhu, G. Richmond, J. Melhorn, N.P. Talbot, G.A.D. Ritchie, P.A. Robbins, I.D. Pavord, H. Ashdown, N. Petousi
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
XeLHC: Initial Impressions From Hyperpolarized <sup>129</sup>Xe MRI Measurements of Regional Lung Health in Participants From the Lung Health Cohort
Authors: P.J. Niedbalski, I. Mali, S.B. Fain, A. Hahn, A.P. Comellas, B. Driehuys, D. Mummy, L.G. Que, M. Castro
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Pembrolizumab-induced Reactivation of ANCA-associated Vasculitis Presenting With Hemoptysis and Pauci-immune Glomerulonephritis in a Patient With Metastatic Non-small Cell Lung Cancer
Authors: T. Nogueira Gomes, S. Macrae, L. Hutchins, A. Krishnan
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Assessing the Stability of the Healthy Never-smoking Subgroup Over 12 Years and Its Effect on Reference Equations for Lung Function in a Prospective Cohort Study: The Multi-ethnic Study of Atherosclerosis Lung Study
Authors: K. Buschur, P. Balte, E.A. Hermann, J.L. Hankinson, N.B. Allen, A.G. Bertoni, J. Pankow, W.S. Post, B.M. Smith, K. Watson, R.G.G. Barr
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Spatial Transcriptomics Identify Region-specific Pathogenesis of Bronchiolar and Alveolar Disease in Primary Ciliary Dyskinesia Lungs
Authors: M. Saito, T. Asakura, H. Dang, H. Murano, K. Okuda, M. Markovetz, R.C. Gilmore, G.D.L. Cruz, M. Chua, A. Khoor, S.J. McCall, L.D. Snyder, J. Sembrat, F.B. Askin, S.L. Brody, A. Shoemark, A. Livraghi-Butrico, S.H. Randell, W.K. O'Neal, R.C. Boucher
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Sustained Activation of Yap/Taz After Injury Leads to Aberrant Alveolar Differentiation and Fibrotic Remodeling
Authors: I.P. Gaona, A.S. Mccall, G.T. DiGiovanni, S.S. Gutor, U.K. Singha, N.M. Geis, A.C. Colvard, T.P. Sherrill, T.S. Blackwell, J.A. Kropski, J.J. Gokey
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Emphysema Severity Index (ESI) in a General Population Sample: A New Spirometric Parameter
Authors: G. Viegi, S. Maio, L. Carrozzi, S. Baldacci, F. Pistelli, G. Sarno, A. Angino, I. Stanisci, S. Tagliaferro, P. Silvi, G. Cenerini, M. Occhipinti, M. Paoletti, M. Pistolesi
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Tirzepatide as Compared with Semaglutide for the Treatment of Obesity
Authors: Louis J. Aronne, Deborah Bade Horn, Carel W. le Roux, Wayne Ho, Beverly L. Falcon, Elisa Gomez Valderas, Sagar Das, Clare J. Lee, Leonard C. Glass, Cagri Senyucel, Julia P. Dunn
Journal: New England Journal of Medicine (2025)

Tirzepatide and semaglutide are highly effective medications for obesity management. The efficacy and safety of tirzepatide as compared with semaglutide in adults with obesity but without type 2 diabetes is unknown. In this phase 3b, open-label, controlled trial, adult participants with obesity but without type 2 diabetes were randomly assigned in a 1:1 ratio to receive the maximum tolerated dose of tirzepatide (10 mg or 15 mg) or the maximum tolerated dose of semaglutide (1.7 mg or 2.4 mg) subcutaneously once weekly for 72 weeks. The primary end point was the percent change in weight from baseline to week 72. Key secondary end points included weight reductions of at least 10%, 15%, 20%, and 25% and a change in waist circumference from baseline to week 72. A total of 751 participants underwent randomization. The least-squares mean percent change in weight at week 72 was -20.2% (95% confidence interval [CI], -21.4 to -19.1) with tirzepatide and -13.7% (95% CI, -14.9 to -12.6) with semaglutide (P<0.001). The least-squares mean change in waist circumference was -18.4 cm (95% CI, -19.6 to -17.2) with tirzepatide and -13.0 cm (95% CI, -14.3 to -11.7) with semaglutide (P<0.001). Participants in the tirzepatide group were more likely than those in the semaglutide group to have weight reductions of at least 10%, 15%, 20%, and 25%. The most common adverse events in both treatment groups were gastrointestinal, and most were mild to moderate in severity and occurred primarily during dose escalation. Among participants with obesity but without diabetes, treatment with tirzepatide was superior to treatment with semaglutide with respect to reduction in body weight and waist circumference at week 72. (Funded by Eli Lilly; SURMOUNT-5 ClinicalTrials.gov number, NCT05822830.).
Topical ruxolitinib for treatment of mycosis fungoides and lymphomatoid papulosis
Authors: Marion Wobser, Matthias Goebeler, Andreas Rosenwald, Katja Maurus
Journal: British Journal of Dermatology (2025)

Activation of the JAK/STAT signalling pathway plays a pivotal role in the pathogenesis of cutaneous T-cell lymphomas. Therefore, inhibition of this cardinal pathways is a promising novel treatment option. We herein report the first evidence of rapid response of lymphomatoid papulosis as well as mycosis fungoides upon topical application of the JAK-inhibitor ruxolitinib.
Inhibition of SOD1 trimerization is a novel drug target for ALS disease
Authors: Tae-Gyun Woo, Jin Han, Yuju Kim, Young Jun Hwang, Mua Lee, So-Mi Kang, Soyoung Park, Yeongseon Ji, Yeon-Ho Chung, Songyoung Baek, Eunbyeol Shin, None Minju-Kim, Hyewon Jang, Yun-Jeong Shin, Yonghoon Kwon, Bae-Hoon Kim, Bum-Joon Park
Journal: Translational Neurodegeneration (2025)

Abstract Background Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease that begins with motor neuron death in the spinal cord and cerebral cortex, ultimately resulting in death from respiratory distress (breathing failure). About 90% of ALS cases are sporadic, and 10% of ALS cases are of the inherited type with a genetic cause. About 150 different gene mutations have been reported so far. SOD1 is a well-identified gene associated with ALS. Indeed, SOD1 aggregation has been reported in ALS patients, but the mechanism of SOD1 aggregation remains unclear. Our previous work showed that inhibiting SOD1 aggregation with a hit compound (PRG-A-01) could reduce the SOD1-induced cytotoxicity and extend the lifespan of ALS mouse model (SOD1 G93A−Tg ). However, the low bioavailability and rapid degradation of the compound in vivo necessitates the development of a more effective candidate. We generated different derivatives and finally obtained the most potential drug candidate, PRG-A-04. Methods Neuronal cell lines were transfected with the mutant SOD1 expression vector and incubated with PRG-A-04. SOD1 aggregation was examined by SOD1 oligomerization assay, immunofluorescence and dot blot assay. The interaction between GST-conjugated SOD1 recombinant proteins and PRG-A-04 was identified using LC–MS/MS and GST pull-down assay. To check the in vivo therapeutic effect of PRG-A-04, SOD1 G93A−Tg mice were injected with PRG-A-04; then behavioral test, histological analysis and microarray were performed. Results PRG-A-04 demonstrated favorable pharmacokinetics including high bioavailability and significant blood–brain barrier penetration. Indeed, oral administration of PRG-A-04 in ALS mouse model inhibited the aggregation of SOD1 in the spinal cord, protected against neuronal loss, and extended the lifespan of ALS mice by up to 3 weeks. In vitro, PRG-A-04 selectively bound to the mutant form of SOD1, but not the wild type, and efficiently inhibited the aggregation caused by SOD1-G147P (a SOD1 trimer stabilizer). Conclusions Our findings underscore the potential of targeting trimeric SOD1 in ALS treatment, positioning PRG-A-04 as a strong drug candidate for both familial and sporadic ALS.
Stress granules: emerging players in neurodegenerative diseases
Authors: Lin Yuan, Li-Hong Mao, Yong-Ye Huang, Tiago F Outeiro, Wen Li, Tuane C R G Vieira, Jia-Yi Li
Journal: Translational Neurodegeneration (2025)

Abstract Stress granules (SGs) are membraneless organelles formed in the cellular cytoplasm under stressful conditions through liquid–liquid phase separation (LLPS). SG assembly can be both dependent and independent of the eIF2α pathway, whereas cellular protein quality control systems mediate SG disassembly. Chaperones and specific domains of RNA-binding proteins strongly contribute to the regulation SG dynamics. Chronic stress, arising in association with aging, may promote persistent SGs that are difficult to disassemble, thereby acting as a potential pathological nidus for protein aggregation in neurodegenerative diseases (NDDs). In this review, we discuss the dynamics of SGs and the factors involved with SG assembly and disassembly. We also highlight the relationship among LLPS, SGs, and the pathogenesis of different NDDs. More importantly, we summarize SG assembly-disassembly, which may be a double-edged sword in the pathophysiology of NDDs. This review aims to provide new insights into the biology and pathology of LLPS, SGs, and NDDs.
Bacteriophage-driven DNA inversions shape bacterial functionality and long-term co-existence in <i>Bacteroides fragilis</i>
Authors: Shaqed Carasso, Roni Keshet-David, Jia Zhang, Haitham Hajjo, Dana Kadosh-Kariti, Tal Gefen, Naama Geva-Zatorsky
Journal: Gut Microbes (2025)

Bacterial genomic DNA inversions, which govern molecular phase-variations, provide the bacteria with functional plasticity and phenotypic diversity. These targeted rearrangements enable bacteria to respond to environmental challenges, such as bacteriophage predation, evading immune detection or gut colonization. This study investigated the short- and long-term effects of the lytic phage Barc2635 on the functional plasticity of Bacteroides fragilis, a gut commensal. Germ-free mice were colonized with B. fragilis and exposed to Barc2635 to identify genomic alterations driving phenotypic changes. Phage exposure triggered dynamic and prolonged bacterial responses, including significant shifts in phase-variable regions (PVRs), particularly in promoter orientations of polysaccharide biosynthesis loci. These shifts coincided with increased entropy in PVR inversion ratios, reflecting heightened genomic variability. In contrast, B. fragilis in control mice exhibited stable genomic configurations after gut adaptation. The phase-variable Type 1 restriction-modification system, which affects broad gene expression patterns, showed variability in both groups. However, phage-exposed bacteria displayed more restrained variability, suggesting phage-derived selection pressures. Our findings reveal that B. fragilis employs DNA inversions to adapt rapidly to phage exposure and colonization, highlighting a potential mechanism by which genomic variability contributes to its response to phage. This study demonstrates gut bacterial genomic and phenotypic plasticity upon exposure to the mammalian host and to bacteriophages.
Successful Autologous Hematopoietic Stem Cell Transplant While on Venovenous Extracorporeal Membrane Oxygenation Support
Authors: C. Yugawa, B. Kwok, A.S. Lubinsky, N.E. Amoroso, R.M. Goldenberg, J.C. Chan, J. Hsu
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
When Bleeding Lungs Meet Autoimmune Chaos: A Case of Diffuse Alveolar Hemorrhage in Adenovirus-induced Catastrophic Antiphospholipid Syndrome
Authors: K. Moore, E. Grishkevich-Karpicki, R.Z. Khan, W.T. Kong, H. Alsmaan
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
A Case of Cardiac Tamponade Following Catheter Ablation for Atrial Fibrillation While on Continuous Anticoagulation
Authors: N. Patel, S. Hassan, S.H. Gandhamaneni, R. Al-Ahmad, L. Philip, L. Morrison, S. Khawam, P. Patel, M.P. Goudie
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Persistent Hypoxia in Neurofibromatosis Type 1 With Posterior Mediastinal Mass: The Role of Intracardiac Shunt Detection Via Transcranial Doppler
Authors: P. Vaidya, C. Davenport, J. Kepfinger, A. Sreekantan Nair, J. Boehmler
Journal: American Journal of Respiratory and Critical Care Medicine (2025)
Antibacterial MXenes: An emerging non-antibiotic paradigm for surface engineering of orthopedic and dental implants
Authors: Sathishkumar Gnanasekar, Xiaodong He, Bruna E. Nagay, Kun Xu, Xi Rao, Shun Duan, Selvakumar Murugesan, Valentim A.R. Barão, En-Tang Kang, Liqun Xu
Journal: Bioactive Materials (2025)
Prognostic Significance of Myocardial Ischemia during Exercise Echocardiography in the Absence of Angiographic Evidence of Obstructive Coronary Disease
Authors: Alaa Mabrouk Salem Omar, Swiri Konje, Ga Hee Kim, Robert Leber, Nitin Barman, Edgar Argulian
Journal:  (2025)

We studied the prognostic significance of myocardial ischaemia during exercise stress echocardiography (ExE) in the absence of angiographic evidence of obstructive coronary artery disease (CAD) in a contemporary cohort of patients. We retrospectively enrolled 84 patients who underwent ExE and had exercise-induced myocardial ischaemia followed by angiographic coronary evaluation. Fifty-one (61%) patients had non-obstructive CAD (iNOCAD), and 33 (39%) had normal coronaries (iNC). iNC and NOCAD patients were propensity matched to 99 and 153 patients with non-ischaemic ExE, respectively. Compared to iNOCAD, iNC patients were younger (60.9 ± 10.4 vs. 68 ± 8.9 years, P = 0.002) and predominantly women (76% vs. 47%, P = 0.009). Ejection fraction (57 ± 9.4 vs. 56.4 ± 6, P = 0.776) as well as other clinical and demographic variables were similar. During median follow-up of 3.2 years, there were 27 composite adverse cardiovascular events (1 death, 10 acute chest pain events, 2 strokes, and 21 cardiac hospitalizations). iNC was associated with a higher risk of acute chest pain (HR: 19.0, 95% CI: 3.7-93) and the composite adverse outcome (HR: 3.3, 95% CI: 1.7-6.6), compared to matched patients. Similarly, iNOCAD was associated with a higher risk of the composite outcome (HR: 2.2, 95% CI: 1.2-4.2). Ischaemic ExE in the absence of angiographically obstructive CAD carries an elevated risk of adverse cardiovascular events necessitating medical optimization and close follow-up for progression.
Deciphering the role of RNA in regulating CTCF’s DNA binding affinity in leukemia cells
Authors: Judith Hyle, Wenjie Qi, Mohamed Nadhir Djekidel, Wojciech Rosikiewicz, Beisi Xu, Chunliang Li
Journal: Genome biology (2025)

Abstract Background CTCF, a highly studied transcription factor, is essential for chromatin interaction maintenance. Several independent studies report that CTCF interacts with RNAs in vitro and in cells. Yet continuous debates about the authenticity of the RNA-binding affinity of CTCF and its biological role remain in large part due to limited research techniques available, such as CLIP-seq. Result Here, we investigate RNA’s role in CTCF’s transcription factor function through its chromatin occupancy. To systematically explore whether RNAs affect CTCF’s ability to bind DNA, we perturb CTCF-RNA interactions by three independent approaches and examine CTCF genome occupancy by ChIP-seq. Although RNase A and triptolide treatment each affect a certain number of CTCF-binding peaks, few peaks overlap between treatment groups indicating the effect of RNA in regulating CTCF’s DNA binding affinity is variable between loci. In addition, limited transcriptional or chromatin accessibility changes occur between cells expressing wild-type CTCF or CTCF lacking the RNA binding region. Conclusion Our data provide a complementary approach and in silico evidence to consider the significance of RNA affecting CTCF’s DNA binding affinity globally.
Corrigendum to “Surface modification of electrospun fibers with mechano-growth factor for mitigating the foreign-body reaction” [Bioact. Mater. 6 (9) (2021) 2983–2998]
Authors: Yang Song, Linhao Li, Weikang Zhao, Yuna Qian, Lili Dong, Yunnan Fang, Li Yang, Yubo Fan
Journal: Bioactive Materials (2025)
Dental follicle stem cell-derived small extracellular vesicles ameliorate pulpitis by reprogramming macrophage metabolism
Authors: Jun Tian, Yaxin Lou, Mengjie Li, Yihong Duan, He Liu, Chanchan Chen, Yu Qiu, Weiyang Chen, Chunfeng Pang, Yuhua Xiong, Ya Shen, Xi Wei
Journal: Bioactive Materials (2025)