P1624: THE ROLE OF GENETIC POLYMORPHISMS OF THE HEMOSTASIS SYSTEM IN WOMEN WITH DIFFERENT OBSTETRIC ANAMNESIS
Authors: Anastasia Zagarskikh
Journal: HemaSphere (2023)

Topic: 33. Bleeding disorders (congenital and acquired) Background: Genetically determined thrombophilia is considered one of the causes of venous thromboembolism and gestational disorders. However, the need for screening for polymorphic genes of the hemostasis system in women planning pregnancy does not currently have a reliable scientific justification. Aims: To evaluate the polymorphism of genes of the hemostasis system in women with reproductive failures. Methods: The polymorphism of genes of the hemostasis system (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 G>T, FGB: -455 G>A, ITGA2: 807 C>T, ITGB3: 1565 T>C, PAI-1: -675 5G>4G) in 288 women with reproductive disorders was studied. Depending on the diagnosis, the patients were divided into several groups: 1 (n=15) with female infertility, unspecified (ICD-10: N97.9) (other factors leading to infertility are excluded; the cause of infertility is unknown); 2 (n=180) with female infertility of other origin (N97.8) (combination of female factors, combination of female and male factors)); 3 (n=20) with two complete or unspecified abortions without complications (O03.9); 4 (n=73) with habitual aborter (N96) (three or more miscarriages). The comparison group included 105 women with normal conception and pregnancy, who had 2 or more children. Detection of polymorphisms was carried out with reagents and equipment from DNA-Technology (Moscow, Russia).Statistical data processing used the χ2 test. Results: The distribution of polymorphic genes of the hemostasis system is presented in the table. Statistically significant differences in the prevalence of allele C of the ITGB3 gene 1565 T>C were established. In the second group of women, it was 35.5%, in the comparison group - 22.9% (p<0.05). In other groups of women with reproductive disorders, there were no significant differences in the frequency of occurrence of genes of the hemostasis system. Table - Detection of polymorphism of genes of the hemostasis system (%) in women - Polymorphism Genotypes Comparison group (n=105) 1 group (n=15) 2 group (n=180) 3 group (n=20) 4 group (n=73) F2: 20210 G>A G/G 97,1 86,7 96,1 95,0 97,3 G/A+A/A 2,9 13,4 3,9 5,0 2,7 F5: 1691 G>A G/G 94,3 86,7 92,2 90,0 97,3 G/A+A/A 5,7 13,3 7,8 10,0 2,7 F7:10976 G>A G/G 77,1 73,3 72,8 70,0 83,6 G/A+A/A 22,9 26,7 27,2 30,0 16,4 F13: 103 (163) G>T G/G 46,7 46,7 46,2 55,0 52,1 G/T+T/Т 53,3 53,3 56,7 45,0 47,9 FGB:-455 G>A G/G 51,4 60,0 53,9 65,0 57,5 G/A+A/A 48,6 40,0 46,1 35,0 42,5 ITGА2: 807 C>T C/C 41,6 26,7 35,9 20,0 34,3 C/T+T/T 58,4 73,3 67,1 80,0 65,7 ITGВ3: 1565 T>C T/T 77,1 80,0 64,4 75,0 68,5 T/C+C/C 22,9 20,0 35,5* 25,0 31,5 PAI-1: -675 5G>4G 5G/5G 17,1 26,7 15,6 25,0 12,3 5G/4G+4G/4G 82,9 63,3 84,5 75,0 87,7 Note: * p<0.05, statistically significant differences between groups Summary/Conclusion: The presence of allele C of the ITGB3 gene: 1565 T>C is associated with the risk of developing female infertility. Influence of other hemostasis genes (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 G>T, FGB: -455 G>A, ITGA2: 807 C>T, PAI- 1: -675 5G>4G) on the occurrence of reproductive disorders is not statistically significant (p>0.05). Keywords: Gene polymorphism, Hemostasis, Pregnancy