tbuckley/cabot-search huggingface dataset:
Tumor microenvironment targeting system for glioma treatment via fusion cell membrane coating nanotechnology
Authors: Junning Ma, Lisi Dai, Jianbo Yu, Hui Cao, Youmei Bao, JiaJia Hu, Lihui Zhou, Jiqi Yang, Adame Sofia, Hongwei Chen, Fan Wu, Zhikai Xie, Wenqi Qian, Renya Zhan
Journal: Biomaterials (2023)

The tumor microenvironment (TME), comprising cancer cells and stroma, plays a significant role in determining clinical outcomes, which makes targeting cancer cells in the TME an important area of research. One way in which cancer cells in the TME can be specifically targeted is by coating drug-encapsulated nanoparticles (NPs) with homotypic cancer cell membranes. However, incomplete targeting is inevitable for biomimetic nanoformulations coated with only cancer cell membranes because of the inherent heterogeneity of the TME. After observing the structural connection between glioma-associated stromal cells (GASCs) and glioma cells from a clinic, we designed a novel drug delivery system that targets the TME by coating polylactic-co-glycolic acid (PLGA) NPs with GASC-glioma cell fusion cell (SG cell) membranes. The resulting SGNPs inherited membrane proteins from both the glioma membrane and GASC membrane, significantly enhancing the tumor targeting efficiency compared to nanoformulations coated with cancer cell membranes alone. We further demonstrated that encapsulation of temozolomide (TMZ) improved the therapeutic efficacy of TMZ in both heterotopic and orthotopic glioma mouse models. Owing to its significant efficacy, our TME-targeting nanoplatform has potential for clinical applications in the treatment of various cancers.
Cleaving It All Behind: ADAMTS7 Degrades Factor H
Authors: Peter S. Heeger, Paolo Cravedi
Journal: Journal of the American Society of Nephrology (2023)

1Department of Medicine, Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, California 2Department of Medicine, Translational Transplant Research Center, Icahn School of Medicine at Mount Sinai, New York, New York Correspondence: Dr. Paolo Cravedi, Icahn School of Medicine at Mount Sinai, 1 Levy Place, New York, NY 10029. Email: [email protected] See related article, "ADAMTS7-Mediated Complement Factor H Degradation Potentiates Complement Activation to Cause Renal Injuries," on pages 291–308.
Identification of anti-Mycobacterium tuberculosis agents targeting the interaction of bacterial division proteins FtsZ and SepFe
Authors: Hongjuan Zhang, Ying Chen, Yu Zhang, Luyao Qiao, Xiangyin Chi, Yanxing Han, Yuan Lin, Shuyi Si, Jiandong Jiang
Journal: Acta Pharmaceutica Sinica B (2023)

Tuberculosis (TB) is one of the deadly diseases caused by Mycobacterium tuberculosis (Mtb), which presents a significant public health challenge. Treatment of TB relies on the combination of several anti-TB drugs to create shorter and safer regimens. Therefore, new anti-TB agents working by different mechanisms are urgently needed. FtsZ, a tubulin-like protein with GTPase activity, forms a dynamic Z-ring in cell division. Most of FtsZ inhibitors are designed to inhibit GTPase activity. In Mtb, the function of Z-ring is modulated by SepF, a FtsZ binding protein. The FtsZ/SepF interaction is essential for FtsZ bundling and localization at the site of division. Here, we established a yeast two-hybrid based screening system to identify inhibitors of FtsZ/SepF interaction in M. tuberculosis. Using this system, we found compound T0349 showing strong anti-Mtb activity but with low toxicity to other bacteria strains and mice. Moreover, we have demonstrated that T0349 binds specifically to SepF to block FtsZ/SepF interaction by GST pull-down, fluorescence polarization (FP), surface plasmon resonance (SPR) and CRISPRi knockdown assays. Furthermore, T0349 can inhibit bacterial cell division by inducing filamentation and abnormal septum. Our data demonstrated that FtsZ/SepF interaction is a promising anti-TB drug target for identifying agents with novel mechanisms.
AKT inhibitor Hu7691 induces differentiation of neuroblastoma cells
Authors: Shaowei Bing, Senfeng Xiang, Zhimei Xia, Yilong Wang, Zhonghai Guan, Jinxin Che, Aixiao Xu, Xiaowu Dong, Ji Cao, Bo Yang, Jinhu Wang, Qiaojun He, Meidan Ying
Journal: Acta Pharmaceutica Sinica B (2023)

While neuroblastoma accounts for 15% of childhood tumor-related deaths, treatments against neuroblastoma remain scarce and mainly consist of cytotoxic chemotherapeutic drugs. Currently, maintenance therapy of differentiation induction is the standard of care for neuroblastoma patients in clinical, especially high-risk patients. However, differentiation therapy is not used as a first-line treatment for neuroblastoma due to low efficacy, unclear mechanism, and few drug options. Through compound library screening, we accidently found the potential differentiation-inducing effect of AKT inhibitor Hu7691. The protein kinase B (AKT) pathway is an important signaling pathway for regulating tumorigenesis and neural differentiation, yet the relation between the AKT pathway and neuroblastoma differentiation remains unclear. Here, we reveal the anti-proliferation and neurogenesis effect of Hu7691 on multiple neuroblastoma cell lines. Further evidence including neurites outgrowth, cell cycle arrest, and differentiation mRNA marker clarified the differentiation-inducing effect of Hu7691. Meanwhile, with the introduction of other AKT inhibitors, it is now clear that multiple AKT inhibitors can induce neuroblastoma differentiation. Furthermore, silencing AKT was found to have the effect of inducing neuroblastoma differentiation. Finally, confirmation of the therapeutic effects of Hu7691 is dependent on inducing differentiation in vivo, suggesting that Hu7691 is a potential molecule against neuroblastoma. Through this study, we not only define the key role of AKT in the progression of neuroblastoma differentiation but also provide potential drugs and key targets for the application of differentiation therapies for neuroblastoma clinically.
Biodegradable Stenting in a Patient With a Refractory Biliary Stricture
Authors: Martha M. Kirstein, Carsten Engelke, Philipp Solbach
Journal: Clinical Gastroenterology and Hepatology (2023)

A 67-year-old male patient underwent atypical hepatic resection of segments 2, 3, and 7 with hepaticojejunostomy due to Caroli disease. Two months later, the patient presented with cholestasis and cholangitis most likely due to anastomotic stricture. The patient was treated with 8.5 French (F), Yamakawa type, percutaneous transhepatic cholangiodrainage (PTCD) (PerkuBil, Pflugbeil) as the hepaticojejunostomy could not be reached via endoscopy using colonoscope, balloon, and motorized power spiral scope (Figure A). Percutaneous cholangioscopy revealed a stricture and biopsies were obtained (SpyGlass Discover, Boston Scientific). Histopathologic analysis revealed reactive polypoid granulation tissue with no evidence of malignancy. The PTCD was sequentially expanded to 18F. Two PTCD withdrawal attempts failed, resulting in recurrent cholangitis and the need for reinterventions. In total, the patient was treated with PTCD for 36 months. To improve the quality of life without repeated interventions, a custom-built, biliary, biodegradable stent (BDS) made of polydioxanone was inserted under radiological and cholangioscopic guidance (SX-ELLA, 10 mm/60 mm; 3 radiopaque markers, ELLA-CS/Leufen Medical GmbH) (Figure B–C). To avoid intervention-related cholangitis, an 8.5F PTCD (Cook Medical) was inserted through the BDS (Figure D). On the third day, cholangioscopy revealed early decomposition of the BDS. A shortened 12F Yamakawa PTCD was placed proximal of the BDS to maintain percutaneous access (Figure E). During an observation period of 4 weeks, the patient showed no clinical or laboratory signs of cholangitis. On day 28, contrast imaging by PTCD showed slender bile ducts without stricture and rapid drainage of the contrast into the jejunum (Figure F). Cholangioscopy showed a wide hepaticojejunostomy with remnants of the BDS. Video 1 shows the cholangioscopic findings before placement of the BDS and its degradation during the observation period. This case demonstrates the treatment success of a biliary BDS in a patient with PTCD-refractory stenosis. The authors thank Professor Jens U. Marquardt and Carlos Maass for their support. eyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiI1OWVkODVlYTRhMWQxMWYxODRkMWE1ZGYwOTI5NTM1NiIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNzAyNzAyMTQ5fQ.Kx9kwVlAJ4XmZuugYFyKxjO53tGkelZ9KU4n4aelLYi8uI04Eo7CqsFvEqFJWMEVz5RGGOfzcswB4keex23LwCP70ZiCTr2WvsY0rqBd4zJ6s82vtVPgg-ZQgfAd0xnGMTY-hS52mky_3UOCIk_xxOzeT5fTkdIidEmn8yGzSNXqISjTNrmbQwAvds_UehMzKxKBG9pENQMEyQjRSx3GLO3KOhP70J2Zq8SQSUXAieSTDhlBUd17BuXZvi-iehSmlYUkNMALvn9z1FktOQTCRUXKhvSlfcqpKoJZyLIGac_prwkzpXI6HZ7PtCvGxe9c3hEE-dffiaT6Gz8VJHWQZw Download .mp4 (15.18 MB) Help with .mp4 files Video 1Cholangioscopy showing the stricture prior intervention and the placement of the BDS at day 0, as well as the findings on day 3 and day 28.
Endoscopic vacuum therapy in a patient without nasal access
Authors: Marcelo Simas de Lima, Deborah Marques Centeno, Rafael Utimura Sueta, Pastor Joaquin Ortiz Mendieta, Julia Mayumi Gregorio, Ricardo Sato Uemura, Fauze Maluf-Filho
Journal: Endoscopy (2023)

Usually, upper gastrointestinal (GI) vacuum therapy is delivered through a nasal tube [1]. Herein we report a case in which this access route was not feasible. A 44-year-old man with a previous medical history of a T4aN2M0 extensive squamous-cell lip carcinoma, treated with neoadjuvant chemoradiotherapy followed by tumor resection (including rhinectomy) with surgical defect reconstruction 3 years before admission, was admitted owing to abdominal pain. Computed tomography showed a large pneumoperitoneum. The patient was submitted to an exploratory laparotomy, and a perforated duodenal ulcer was diagnosed and sutured.
Pretraction-assisted endoscopic submucosal dissection for the treatment of a rectal neuroendocrine tumor
Authors: Xiaoxiong Guo, Yunxin Chen, Miao Liu, Sihan Zhang, Canmei Zhong, Aijin Chen, Fenglin Chen
Journal: Endoscopy (2023)

Minor rectal neuroendocrine tumors (NETs) can be resected endoscopically using various procedures. Nevertheless, there remains controversy with regard to which of the possible endoscopic approaches is the most effective [1] [2] [3]. As revealed by relevant reports, endoscopic submucosal dissection (ESD) is an effective and safe technique for the treatment of rectal NETs; however, ESD requires physicians to have more surgical skills and operative times are longer than for other endoscopic resection techniques [2]. A pretraction-assisted ESD technique for small rectal NETs was therefore created to simplify the process and facilitate the operation ([Video 1]).
Pregnancy and neonatal safety outcomes of timing of initiation of daily oral tenofovir disoproxil fumarate and emtricitabine pre-exposure prophylaxis for HIV prevention (CAP016): an open-label, randomised, non-inferiority trial
Authors: Dhayendre Moodley, Carl Lombard, Vani Govender, Megeshinee Naidoo, Alicia C Desmond, Kimesh Naidoo, Ottacia Mhlongo, Motshedisi Sebitloane, Marie-Louise Newell, Richard Clark, James F Rooney, Glenda Gray, Dhayendre Moodley, Carl Lombard, Vani Govender, Megeshinee Naidoo, Alicia C Desmond, Kimesh L Naidoo, Ottacia Mhlongo, Motshedisi Sebitloane, Marie-Louise Newell, Richard Clark, James F Rooney, Glenda E Gray, Linda Ngaleka, Natasha Pillay, Samkelo Booi, Natasha Samsunder, Lorna Pillay, Rosemary Gray, Rosemary Gazu, Thandeka Nkosi
Journal: The Lancet HIV (2023)
Endoscopic submucosal tunnel dissection with an elastic traction device for a circumferential superficial esophageal neoplasm
Authors: Nan Ru, Enqiang Linghu, Ningli Chai
Journal: Endoscopy (2023)

Endoscopic submucosal dissection (ESD) is an established treatment for superficial esophageal squamous cell neoplasms (SESCNs) [1] [2]. However, it is time-consuming and challenging for large SESCNs, especially circumferential lesions. Endoscopic submucosal tunnel dissection (ESTD) has been reported as an effective technique for circumferential SESCNs [3] [4]. Herein, we report a case of traction-assisted ESTD using a novel elastic traction device (Micro-Tech, Nanjing, China), consisting of a rotatable soft-tissue clip and an elastic double ring attached to the clip arm ([Fig. 1]).
Outcome predictors and patient progress following delivery in pregnant and postpartum patients with severe COVID-19 pneumonitis in intensive care units in Israel (OB-COVICU): a nationwide cohort study
Authors: Elena Fatnic, Nikole Lee Blanco, Roman Cobiletchi, Esty Goldberger, Aharon Tevet, Ori Galante, Sigal Sviri, Tali Bdolah-Abram, Baruch M Batzofin, Reuven Pizov, Sharon Einav, Charles L Sprung, P Vernon van Heerden, Yehuda Ginosar, Tamer Abu Jreis, Susan Burrows, Ariel Berkowitz, Shimon Firman, Nicolas Galarza, Rawhi Hashem, Natasha Kuzmina, Stephane Ledot, Dana Wolf, Hadar Golan-Berman, Charles Weissman, Ronit Calderon-Margalit, Moshe Matan, Daniel J Jakobson, Arie Eden, Yael Lichter, Meital Zikry Deitch, Elena Kishinevsky, Shani Kaptzon, Liran Statlender, Chloe Mimouni, Yaron Bar-Lavie, Roy Ilan, Or Assouline, Larisa Yakobson, Dmitry Budman, Arie Soroksky
Journal: The Lancet Respiratory Medicine (2023)
Methylprednisolone versus intravenous immunoglobulins in children with paediatric inflammatory multisystem syndrome temporally associated with SARS-CoV-2 (PIMS-TS): an open-label, multicentre, randomised trial
Authors: Tatjana Welzel, Andrew Atkinson, Nina Schöbi, Maya C Andre, Douggl G N Bailey, Geraldine Blanchard-Rohner, Michael Buettcher, Serge Grazioli, Henrik Koehler, Marie-Helene Perez, Johannes Trück, Federica Vanoni, Petra Zimmermann, Carlos Sanchez, Julia A Bielicki, Luregn J Schlapbach, Andreas Bieri, Spyridoula Gysi, Indra Janz, Birgit Donner, Jürg Hammer, Ulrich Heininger, Malte Kohns Vasconcelos, Nicole Mettauer, Alexandra Meyer, Diana Reppucci, Chloé Schlaeppi, Daniel Trachsel, Nina Vaezipour, Clemens von Kalckreuth, Andreas Woerner, Andreas Zutter, Lisa Kottanattu, Calogero Mazzara, Alessia Severi Conti, Christoph Aebi, Philipp K A Agyeman, Andrea Duppenthaler, Martin Glöckler, Sabine Pallivathukal, Thomas Riedel, Hong-Phuc Cudré-Cung, Mladen Pavlovic, Alice Bordessoule, Arnaud G L'Huillier, Anne-Laure Martin, Angelo Polito, Marie Rohr, Maria Isabel Rodriguez, Noemie Wagner, Vivianne Chanez, Sabrina Bressieux-Degueldre, Thomas Ferry, Michael Hofer, David Longchamp, Julia Natterer, Rebecca Oppenheim, Alex Donas, Sara Germann, Katja Hrup, Daniela Kaiser, Hans Peter Kuen, Michaela Lütolf Erni, Katharina Schwendener Scholl, Janine Stritt, Katharina Wechselberger, Ingrid Beck, André Birkenmaier, Martin Flade, Bjarte Rogdo, Ivo Iglowstein, Philip Lorenz, Tanja Wachinger, Konstanze Zöhrer, Barbara Brotschi, Patrick M Meyer Sauteur, Jana Pachlopnik Schmid, Seraina Prader, Michelle Seiler, Kathrin Weber, Daniela Wütz, Elizabeth Whittaker, Saul N Faust
Journal: The Lancet Child & Adolescent Health (2023)
Three-arm endoscopic mucosal resection for gastric adenocarcinoma of fundic gland type
Authors: Chao Deng, Suhua Wu, Xiaodong Guo, Feng Xu, Chengying Liu, Zhechuan Mei, Song He
Journal: Endoscopy (2023)

A 65-year-old man was diagnosed with intramucosal carcinoma by endoscopy and pathological biopsy; the tumor was 10 mm in size and located in the greater curvature of the gastric body ([Fig. 1 a]). It was difficult to perform endoscopic submucosal dissection (ESD) on the greater curvature of the gastric body. We therefore proposed a three-arm endoscopic mucosal resection (TA-EMR) technique in which two nasal oxygen tubes, about 80 cm in length, were taped to the outside of the endoscope to become a double additional working channel (AWC) ([Video 1]).
ACE2 binding and antibody evasion in enhanced transmissibility of XBB.1.5
Authors: Can Yue, Weiliang Song, Lei Wang, Fanchong Jian, Xiaosu Chen, Fei Gao, Zhongyang Shen, Youchun Wang, Xiangxi Wang, Yunlong Cao
Journal: The Lancet Infectious Diseases (2023)
The association of neonatal SARS-CoV-2 anti-spike protein receptor-binding domain antibodies at delivery with infant SARS-CoV-2 infection under the age of 6 months: a prospective cohort study
Authors: Lital Oz-Alcalay, Eyal Elron, Rotem Davidovich, Gabriel Chodick, Micky Osovsky, Rony Chen, Liat Ashkenazi-Hoffnung
Journal: Clinical Microbiology and Infection (2023)
Prolonged patency of fully covered self-expandable metal stents with an externally anchored plastic stent in distal malignant biliary obstruction
Authors: Jung Won Chun, Sang Myung Woo, Mira Han, Min Woo Lee, Jin Ho Choi, In Rae Cho, Woo Hyun Paik, Woo Jin Lee, Ji Kon Ryu, Yong-Tae Kim, Sang Hyub Lee
Journal: Endoscopy (2023)

Background Fully covered self-expandable metal stents (FCSEMSs) are widely used for endoscopic treatment of distal malignant biliary obstruction (dMBO). We aimed to assess the efficacy of anchoring an external plastic stent to an FCSEMS in dMBO. Methods A multicenter retrospective cohort study was performed in patients with dMBO to compare stent patency between FCSEMSs and FCSEMSs with an externally anchored plastic stent (EPS). For external anchoring, a 7-Fr double-pigtail plastic stent (DPPS) was placed first in the bile duct, then an FCSEMS was deployed side-by-side. Results Among a total of 185 patients, 65 had an FCSEMS alone and 120 had an FCSEMS with an EPS. The median stent patency was significantly longer in the FCSEMS with an EPS group than in the FCSEMS only group (342 vs. 240 days; P = 0.04). The rate of stent migration was significantly lower in the FCSEMS with an EPS group than in the FCSEMS only group (10.8 % vs. 27.7 %; P = 0.01). There were no significant differences in the rates of stent occlusion and adverse events between the two groups. Conclusions A novel and simple technique of anchoring an external plastic stent may decrease the risk of FCSEMS migration and prolong stent patency, without significantly increasing the adverse events rate in dMBO.
Efficacy of short-bending sphincterotome for difficult biliary cannulation in double-balloon enteroscopy-assisted ERCP
Authors: Koh Kitagawa, Akira Mitoro, Fumimasa Tomooka, Shohei Asada, Yukihisa Fujinaga, Norihisa Nishimura, Hitoshi Yoshiji
Journal: Endoscopy (2023)

Double-balloon enteroscopy-assisted endoscopic retrograde cholangiopancreatography (DBE-ERCP) has recently proven useful for patients with surgically altered anatomy [1] [2]. However, selective bile duct cannulation (SBC) via the native papilla is difficult to perform if the scope is positioned tangentially to the papilla [3]. SBC is difficult in DBE-ERCP because a forward-viewing scope is used without a forceps elevator rather than a duodenoscope, which is utilized in normal ERCP [4]. Thus, we present a novel technique using a short-bending sphincterotome in DBE-ERCP for difficult bile duct cannulation.
Unique endoscopic and histological findings of early gastric cancer with surrounding map-like redness detected 10 years after successful Helicobacter pylori eradication
Authors: Yohei Koyama, Takashi Kawai, Fumito Yamanishi, Sho Matsumoto, Masakatsu Fukuzawa, Jun Matsubayashi, Takao Itoi
Journal: Endoscopy (2023)

We are indebted to Helena Popiel, Instructor of the Center for International Education and Research of Tokyo Medical University, for her editorial review of the manuscript.
When I use a word . . . . Medical wordbooks
Authors: Jeffrey K Aronson
Journal: BMJ (2023)

Books in which words are collected, with definitions of their meanings and other information, such as etymologies, are commonly called dictionaries. However, other words are available, and apply to different types of the same thing: alveary, etymologicon, expositor, gazetteer, glossary, gradus, idioticon, lexicon, onomasticon, synonymicon, thesaurus, vocabulary, and wordbook. Medical dictionaries include Dunglison’s <i>New Dictionary of Medical Science</i> (1833), which is really a lexicon, the <i>New Sydenham Society’s Lexicon</i>, which is really a wordbook, and Dorland’s <i>American Illustrated Medical Dictionary</i> (1900), which is a dictionary.
Creating and improving highly effective endoscopic teams
Authors: Samir C. Grover
Journal: Endoscopy (2023)

In this issue of Endoscopy, Ravindran and colleagues from the TEAM-ENTS working group present a framework for core relevant non-technical skills (NTS) for endoscopy teams, derived from a two-phase qualitative mixed-method process involving the development of an NTS taxonomy for endoscopy teams, and refinement into a behavioral marker system (BMS) using Delphi methodology [1]. The authors’ approach in this paper is robust – beginning with a systematic review, which included 14 team-based BMSs from other health disciplines – and has resulted in the creation of an endoscopy-specific team BMS that is applicable in numerous circumstances, ranging from generic everyday diagnostic endoscopy to emergency lifesaving procedures.
Secukinumab in moderate-to-severe hidradenitis suppurativa (SUNSHINE and SUNRISE): week 16 and week 52 results of two identical, multicentre, randomised, placebo-controlled, double-blind phase 3 trials
Authors: Alexa B Kimball, Gregor B E Jemec, Afsaneh Alavi, Ziad Reguiai, Alice B Gottlieb, Falk G Bechara, Carle Paul, Evangelos J Giamarellos Bourboulis, Axel P Villani, Andreas Schwinn, Franziska Ruëff, Larisha Pillay Ramaya, Adam Reich, Ines Lobo, Rodney Sinclair, Thierry Passeron, Antonio Martorell, Pedro Mendes-Bastos, Georgios Kokolakis, Pierre-Andre Becherel, Magdalena B Wozniak, Angela Llobet Martinez, Xiaoling Wei, Lorenz Uhlmann, Anna Passera, Deborah Keefe, Ruvie Martin, Clarice Field, Li Chen, Marc Vandemeulebroecke, Shoba Ravichandran, Elisa Muscianisi
Journal: The Lancet (2023)
Validation of the Prognostic Usefulness of the Gene Expression Profiling Test in Patients with Uveal Melanoma
Authors: Sofia Miguez, Ryan Y. Lee, Alison X. Chan, Patrick C. Demkowicz, Bailey S.C.L. Jones, Christopher P. Long, David H. Abramson, Marcus Bosenberg, Mario Sznol, Harriet Kluger, Michael H. Goldbaum, Jasmine H. Francis, Renelle Pointdujour-Lim, Mathieu F. Bakhoum
Journal: Ophthalmology (2023)
Outcomes in children of women with type 2 diabetes exposed to metformin versus placebo during pregnancy (MiTy Kids): a 24-month follow-up of the MiTy randomised controlled trial
Authors: Denice S Feig, J Johanna Sanchez, Kellie E Murphy, Elizabeth Asztalos, Bernard Zinman, David Simmons, Andrea M Haqq, I George Fantus, Lorraine Lipscombe, Anthony Armson, Jon Barrett, Lois Donovan, Paul Karanicolas, Siobhan Tobin, Kathryn Mangoff, Gail Klein, Yidi Jiang, George Tomlinson, Jill Hamilton
Journal: The Lancet Diabetes & Endocrinology (2023)
New Frontiers in Therapeutics for Interstitial Lung Diseases
Authors: Nathan Mesfin, Ruchika Sangani, Divya A. Shankar, Christine Reardon
Journal: American Journal of Respiratory and Critical Care Medicine (2023)

"New Frontiers in Therapeutics for Interstitial Lung Diseases." American Journal of Respiratory and Critical Care Medicine, 207(8), pp. 1089–1091
Resolving the hematopoietic stem cell state by linking functional and molecular assays
Authors: Maria Jassinskaja, Monika Gonka, David G. Kent
Journal: Blood (2023)

One of the most challenging aspects of stem cell research is the reliance on retrospective assays for ascribing function. This is especially problematic for hematopoietic stem cell (HSC) research in which the current functional assay that formally establishes its HSC identity involves long-term serial transplantation assays that necessitate the destruction of the initial cell state many months before knowing that it was, in fact, an HSC. In combination with the explosion of equally destructive single-cell molecular assays, the paradox facing researchers is how to determine the molecular state of a functional HSC when you cannot concomitantly assess its functional and molecular properties. In this review, we will give a historical overview of the functional and molecular assays in the field, identify new tools that combine molecular and functional readouts in populations of HSCs, and imagine the next generation of computational and molecular profiling tools that may help us better link cell function with molecular state.