tbuckley/cabot-search huggingface dataset:
Abstract 4116: Immune cell checkpoint profiling of solid tumors by multiplex immunofluorescence
Authors: Amanda Finan, Nicolas Goulange, Manon Motte, Maroua Tliba, Alexandra Mace, Jean-Philippe Coton, Domenico Lazzaro, Renaud Burrer
Journal: Cancer Research (2019)

Abstract Immune checkpoint proteins are important regulators in self-tolerance. However, these same molecules also allow cancer cells to evade immune destruction. Checkpoint inhibitor (CKI) blockade therapies that aim at restoring antitumoral immunity have resulted in long-lasting remission in patients with cancers that were previously seen as incurable. However, only a fraction of the patients respond to these treatments. Research is currently focusing on identifying biomarkers that can predict a response to CKI therapies and stratify patient populations. As it is likely that several CKI need to be targeted at once to restore immune responses, numerous clinical trials associating more than one CKI are under evaluation. We have developed a multiplex immunohistochemistry panel, validated by in-depth image analysis, for immune cell checkpoint profiling. The base of the panel consists of CD3/CD8/PD-1/PD-L1 with the capability of adding additional targets including other immune checkpoint (e.g. TIGIT, SIRPα, CTLA-4) or tumor markers. Here we present our validation assay which uses the base panel and the ability to interchange the additional targets. Assay conditions for the individual biomarkers, including antigen retrieval, antibody concentration, panel position, and efficiency of stripping, are optimized. Simplex protocols are analyzed to ensure accuracy and specificity of the staining, success of stripping, and a balance of signal intensities. Validation includes the comparison of staining (% positive cells or % positive area) between a reference protocol and the position of interest. Multiplex optimization consists of staining serial, single stained slides of the individual biomarkers and the multiplex slide in parallel. The staining concordance (% positive cells) is evaluated with imaging software. Repeatability and robustness of the panels are validated by image analysis. This technique gives clinicians the potential to determine candidate patients for CKI single or double therapies and their progress during treatment. The established, rigorous workflow that we have developed allows our customers to adapt the panels to their needs and to have the highest confidence in the consequent images and results related to the profiling of immune cell checkpoints in solid tumors. Citation Format: Amanda Finan, Nicolas Goulange, Manon Motte, Maroua Tliba, Alexandra Mace, Jean-Philippe Coton, Domenico Lazzaro, Renaud Burrer. Immune cell checkpoint profiling of solid tumors by multiplex immunofluorescence [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 4116.
INSENSIBLE PERSPIRATION AND METABOLISM
Journal: JAMA (1928)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
ADEQUATE VERSUS OPTIMAL NUTRITION
Journal: JAMA (1924)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Data Error and Unclear Language
Authors: Douglas Noble, Lawrence Casalino
Journal: JAMA (2013)

Olof Stephansson, MD, PhD; Helle Kieler, MD, PhD; Bengt Haglund, PhD; Miia Artama, PhD; Anders Engeland, PhD; Kari Furu, PhD; Mika Gissler, PhD; Mette Nørgaard, MD, PhD; Rikke Beck Nielsen, MSc; Helga Zoega, PhD; Unnur Valdimarsdóttir, PhD
Book: Menorrhagia
Authors: M A Lumsden
Journal: BMJ (2000)

Eds Shirish S Sheth, Christopher Sutton Isis Medical Media, £84.95, pp 362 ISBN 1 899066 93 4 Rating: ![Graphic][1]</img>![Graphic][2]</img>![Graphic][3]</img> ![Graphic][4]</img> Menorrhagia is a topic of considerable importance to both women and their gynaecologists. It is a common complaint which causes women to attend their general practitioner and, after they have reached a gynaecologist, is often treated by major surgery. It is thus a topic which is worthy of an in-depth text. Menorrhagia is indeed comprehensive, covering all aspects of the subject in considerable depth and including some rather peripheral issues. The contributions are made by experts in the field, but, as this is constantly changing, some information is inevitably out of date already. The book is put together as separate chapters, and experts in the same subject are not necessarily included in the same section as would normally be the case. This makes the book difficult to work through as there seems to be no particular reason for its layout. The different chapters contrast considerably in style and content, reflecting the different approaches of those with particular medical or surgical expertise and of those from different sides of the Atlantic. On reading this book, I think I learnt as much about how gynaecology is practised in the United … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif [4]: /embed/inline-graphic-4.gif
Therapeutic Pancreatic Endoscopy
Authors: K. Mergener, R. A. Kozarek
Journal: Endoscopy (2003)

The role of endoscopic retrograde cholangiopancreatography (ERCP) in the management of pancreatic diseases is continuing to evolve. This article reviews recent publications spanning a wide range of topics related to therapeutic pancreatic endoscopy: Over the last 12 months, several case series have added to the literature on the short-term and long-term effectiveness of endoscopic therapy of pseudocysts, pancreatic abscesses and fistulas. Identification of a communication between pancreatic duct and a pseudocyst has been suggested to predict response to percutaneous drainage. The importance of identifying pancreatic leaks in patients with severe pancreatitis has been stressed. In addition, endotherapy has been reported to be effective in patients with idiopathic chronic pancreatitis. Endoscopic removal of pancreatic stones after extracorporeal lithotripsy has been shown to result in long-term improvement in clinical outcomes in patients with chronic calcific pancreatitis. Other interesting publications addressed new techniques and tricks to achieve access to the difficult pancreatic duct. Finally, no review of pancreatic endotherapy would be complete without a reminder - as recently stated by a National Institutes of Health consensus panel - that there is considerable need for higher-quality and controlled trials in this and other areas of interventional endoscopy.
Integrated phenotyping of the anti-cancer immune response in HIV-associated hepatocellular carcinoma
Authors: David J. Pinato, Takahiro Kaneko, Alejandro Forner, Beatriz Minguez, Edoardo Giovanni Giannini, Alba Díaz, Federica Grillo, Francesco Mauri, Alessia Dalla Pria, Robert D. Goldin, Edoardo Casagrande, Valentina Cacciato, Ayse Akarca, Teresa Marafioti, Sherrie Bhoori, Mark Bower, Norbert Brau, Vincenzo Mazzaferro
Journal: Journal of Hepatology (2020)
The prescription and proscription of chorionic gonadotropin
Authors: R. D. Amelar
Journal: JAMA (1991)

<h3>To the Editor.—</h3> The Council Report, "Medical and Nonmedical Uses of Anabolic-Androgenic Steroids,"<sup>1</sup>concludes with recommendations for legislative and legal initiatives to control the prescription and distribution of anabolicandrogenic steroids. In addition to Alabama, California, and Florida, which are mentioned as having scheduled the drugs under their state controlled substance acts, the New York State legislature in December 1989 passed a law that reclassifies anabolic steroids as Schedule II, controlled substances. This law, which went into effect on January 12, 1990, requires that all anabolic and androgenic steroids be subject to the same inventory and ordering controls as Schedule II narcotics, barbiturates, and related compounds. This includes the requirement that the steroids be prescribed on a New York State triplicate prescription form and that they may not be prescribed or administered without first obtaining the informed consent of the patient or a person legally authorized to give consent on
Call for emergency action to limit global temperature increases, restore biodiversity, and protect health
Authors: Lukoye Atwoli, Abdullah H Baqui, Thomas Benfield, Raffaella Bosurgi, Fiona Godlee, Stephen Hancocks, Richard Horton, Laurie Laybourn-Langton, Carlos Augusto Monteiro, Ian Norman, Kirsten Patrick, Nigel Praities, Marcel G M Olde Rikkert, Eric J Rubin, Peush Sahni, Richard Smith, Nicholas J Talley, Sue Turale, Damián Vázquez
Journal: The Lancet Psychiatry (2021)
Abstract 427: Detection and molecular characterization of circulating tumor cells in advanced head and neck squamous cell carcinoma
Authors: Hiroe Tada, Hideyuki Takahashi, Hiroki Ishii, Yuki Kuwabara-Yokobori, Masato Shino, Kazuaki Chikamatsu
Journal: Cancer Research (2019)

Abstract Malignant properties of tumor cells are genetically and epigenetically altered during tumor development and progression. Moreover, tumor cells rapidly change to treatment resistant phenotype by various therapeutic pressures, and not only become more heterogeneous but also possess metastatic potential. Detection and characterization of circulating tumor cells (CTCs) in the bloodstream of cancer patients have been currently remarked as a valuable tool for real-time evaluation of tumor tissue with phenotypic heterogeneity in the diagnosis and treatment of various cancers, however these have been still challenging. In the present study, we investigated the presence of CTCs and their molecular characterization by the combination of microfiltration system and RT-qPCR method. Peripheral blood samples were collected from 30 patients with advanced head and neck squamous cell carcinoma (HNSCC) and passed through two CellSieveTM microfilters using a low-pressure filtration system. The existence of CTCs collected from the first filter was determined by the expression of epithelial-related genes (KRT19, EPCAM, EGFR, or MET), then the expression of cell proliferation (PIK3CA and CCND1)-, EMT (SNAI1 and VIM)-, cancer stemness (CD44, NANOG, and ALDH1A1)-, and immune regulation (CD47, CD274, and PDCD1LG2)-related genes was analyzed for molecular characterization of CTCs. The gene expression in cells collected from the second filter was used for normalization of target genes. Sixteen (53.3%) of 30 patients were positive for at least one epithelial-related gene. Interestingly, the number of patients with locoregional recurrence or distant metastasis in 16 CTC-positive patients was 7 (43.8%) or 5 (31.3%); whereas that in 14 CTC-negative patients was 2 (14.3%) or 1 (7.1%), respectively. The gene expression profile in CTCs collected from 16 CTC-positive patients has revealed remarkable heterogeneity between HNSCC patients. Of 16 CTC-positive patients, PIK3CA was overexpressed in 56.3%, CCND1 in 43.8%, SNAI1 in 62.5%, VIM in 43.8%, CD44, in 50.0%, NANOG in 31.3%, ALDH1A1 in 56.3%, CD47 in 62.5%, CD274 in 37.5%, and PDCD1LG2 in 31.3%. Our results suggest that CTCs may play an important role in locoregional recurrence as well as distant metastasis in HNSCC. Further molecular profiling at the single-cell level of CTCs is needed to clarify the clinical significance of molecular phenotype of CTCs. Citation Format: Hiroe Tada, Hideyuki Takahashi, Hiroki Ishii, Yuki Kuwabara-Yokobori, Masato Shino, Kazuaki Chikamatsu. Detection and molecular characterization of circulating tumor cells in advanced head and neck squamous cell carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 427.
Correction
Authors: E Moorhouse, Miss Harwood, J Forshaw
Journal: BMJ (1963)

rather than of folic acid.Absorption of vitamin B12 was at the lower limit of normal and was improved by intrinsic factor.However, the absorption was higher than that found in pernicious anaemia, and the presence of free acid in the gastric juice and the normal appearance of the gastric mucosa do not confirm this diagnosis.The marginal absorption of vitamin B12 was probably a factor in the development of deficiency of this vitamin rather than of folic acid.The serum vitamin B 12 level of 120 it~tg./ml. is signi- ficantly low, but it is higher than that usually found in cases with megaloblastic erythropoiesis due to vitamin B12 deficiency.It seems probable that the depletion of vitamin B.2 was greater in the bone marrow than in the serum, and that this was due to increased utilization of the vitamin in the marrow by proliferating myeloma cells.
Abstract 188: Temporal Changes in Morphological Characteristics of Ventricular Fibrillation from Out-of-Hospital Cardiac Arrest in an Asian Community
Authors: Weiting Chen, Nai-Chuan Chen, Yen-Pin Chen, Yi-Chia Lee, Hui-Chih Wang, Chen Lin, Hui-Wen Yang, Yu-Hsin Tseng, Yi-Chung Chang, Chih-Wei Yang, Patrick Chow-In Ko, Wen-Chu Chiang, Liang-Yu Lin, Men-Tzung Lo, Matthew Huei-Ming Ma
Journal: Circulation (2014)

Introduction: Ventricular fibrillation (VF) is the most common arrhythmia causing out-of-hospital cardiac arrest (OOHCA) and defibrillation remains the mainstay of its treatment. Successful defibrillation is linked to VF morphology characteristics, and is affected by VF duration, quality of cardiopulmonary resuscitation (CPR) and emergency medical service (EMS) responsiveness. This study aimed at comparing VF morphology characteristics and their relationships with patients and EMS characteristics, as well as outcomes, in an Asian community. Method: Electrocardiographic recordings of VF signals from automated external defibrillators (AEDs) in 2008 to 2012 were obtained from OOHCA subjects in Taipei city, a metropolitan with 2.65 million residents in 272km2. Amplitude spectrum analysis (AMSA) and DFAα2 were calculated and compared year by year in the study period. Results: A total of 612 OOHCA subjects with VF were included for analysis. Both DFA and AMSA changed significantly over time (χ 2 for trend: -0.128 for DFA, p&lt;0.01; 0.092 for AMSA, p=0.02). There was no change in gender, age, and bystander CPR rates; however, in later years there were more witnessed arrests, shortened EMS responses, and better sustained (&gt;2 hr) return of spontaneous circulation (ROSC) and neurological outcomes (CPC 1&amp;2). The time trend for DFAα2 (OR 0.98, CI 0.96-0.99, p=0.003), but not for AMSA (OR 1.07, CI 0.99-1.14), remained after adjustment for confounders. Both DFAα2 and AMSA were associated with sustained ROSC (DFA OR 0.08, CI 0.04-0.18, p&lt;0.001; AMSA OR 1.18, CI 1.01-1.38, p=0.03) and CPC 1&amp;2 (DFA OR 0.04, CI 0.01-0.16, p&lt;0.001; AMSA OR 1.30, CI 1.07-1.57, p=0.008) in multiple regression models. Conclusions: DFAα2 and AMSA, two important VF characteristics, changed significantly over time in an Asian community, and were associated with improved outcomes. This might reflect improvement in community chain of survival especially among EMS responses and community quality of CPR.
Patient complaints against physicians in the Richmond area during 1973
Authors: C. L. Cooke
Journal: JAMA (1976)

THIRTY-FIVE formal complaints were lodged in writing against members of the Richmond Academy of Medicine during 1973. Each such complaint must be reviewed as a potential malpractice suit, and anything that can be done to prevent the soaring cost of malpractice appears to be warranted.<sup>1-3</sup>This communication identifies some problems in the practice of medicine and makes some suggestions to eliminate them. <h3>Reviewing Complaints</h3> The Mediations Committee of the Richmond Academy of Medicine is a standing committee to deal with complaints of patients against their physicians. The existence of this committee has not been publicized on a regular basis, and complaints have not been solicited but are dealt with as they arise. The only requirement for a complaint to be investigated is that it must be submitted in writing. There is no requirement that the patient himself must submit the complaint. On receipt of the written complaint, a member
Effects of Ultraviolet Light on the Eye: Role of Protective Glasses
Authors: Frederik J. G. M. van Kuijk
Journal: Environmental Health Perspectives (1991)

Global atmospheric changes such as depletion of ozone in the stratosphere are thought to lead to increased levels of ultraviolet radiation on earth. This can have adverse effects on human health, and long-term effects of ultraviolet light on the eye are of increasing concern. Ultraviolet light exposure to the eye has been associated with cataract formation and retinal degeneration. In both cases, it is hypothesized that ultraviolet light can initiate formation of free radicals, which can cause protein modification and lipid peroxidation. Several procedures can be recommended to prevent ultraviolet light damage to the eye, such as the use of suitable protective glasses when outdoors.
Liver scan in hyperlipoproteinemia
Authors: B. A. Sachs
Journal: JAMA (1974)

Liver scans with technetium 99m sulfur colloid were obtained in 48 subjects with hyperlipoproteinemia who were being treated with various lipidlowering drug regimens or were receiving no therapy. Twenty-seven of the 48 liver scans were abnormal, but the abnormalities could not be firmly attributed to a specific lipoprotein phenotype or treatment regimen. Of the patients with elevated triglyceride levels, 71% had abnormal liver scans. Hypertriglyceridemia was present in 55% of the subjects with abnormal liver scans compared to 28% of the subjects in whom the scans were normal. Hyperlipoproteinemia should be considered as a possible associated factor in evaluating an abnormal liver scan.
Predictors of Plasma Concentrations of DDE and PCBs in a Group of U.S. Women
Authors: Francine Laden, Lucas M. Neas, Donna Spiegelman, Susan E. Hankinson, Walter C. Willett, Karen Ireland, Mary S. Wolff, David J. Hunter
Journal: Environmental Health Perspectives (1999)

We evaluated predictors of plasma concentrations of dichlorodiphenyldichloroethylene (DDE), a metabolite of dichlorodiphenyltrichloroethane (DDT), and polychlorinated biphenyls (PCBs) in a group of 240 women, controls from a breast cancer case-control study nested in the Nurses' Health Study. We considered personal attributes such as age, serum cholesterol, region of residence, adiposity, lactation, and dietary intake. DDE levels increased 0.17 ppb/year of age (p = 0.0003), and PCBs increased 0.08 ppb (p = 0.0001). DDE and PCBs increased 0.20 (p = 0.02) and 0.13 ppb (p = 0.001), respectively, per 10 mg/dl serum cholesterol. Women living in the western United States had higher levels of DDE (mean = 11.0 ppb; p = 0.003), and women in the Northeast and Midwest had higher levels of PCBs (mean = 5.6 ppb; p = 0.0002) as compared to women from other parts of the country (mean DDE = 6.3; mean PCBs = 4. 5 ppb). Levels of DDE could not be predicted from consumption of meat, fish, poultry, dairy products, vegetables, fruits, and grains. There was a positive association between fish consumption and PCB concentrations among women in the Northeast and Midwest. Using data from the cases in the nested case-control study to assess the predictive ability of the models, we confirmed that the most reliable predictors of DDE were age and serum cholesterol, and the most important predictors of PCBs were age, serum cholesterol, and residence in the Midwest or Northeast. The null results for the majority of the food variables suggest that specific dietary factors, other than fish, are not currently a substantial contributor to human exposure to DDE and PCBs.
Elsie
Authors: Ron Linder
Journal: JAMA (1983)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Highlights
Journal: JAMA (2013)

Micronutrient deficiencies are common early in human immunodeficiency virus (HIV) infection and are associated with accelerated disease progression.Baum and colleagues examined the effect of daily oral micronutrient supplementation (B vitamins, vitamins C and E [multivitamin]; selenium alone; or multivitamin plus selenium) compared with placebo on disease progression in a randomized trial that enrolled 878 Botswana adults who were antiretroviral therapy naive.The authors report that participants who received the combined multivitamin plus selenium supplement had a reduced risk of immune system decline, morbidity, and disease progression compared with participants who received placebo.