POS0483 THE EFFECT OF VITAMIN D3 AND α-TOCOPHEROL ACETATE IN THE PRECLINICAL MODEL OF SYSTEMIC SCLEROSIS
Authors: B. Doskaliuk, L. Zaiats, R. Yatsyshyn
Journal: Annals of the Rheumatic Diseases (2022)

Background The pathogenesis of systemic sclerosis (SSc) is characterized by complex damage of an organism due to the development of mechanisms of autoimmunity. Dysregulation of immune system, development of fibrosis, and vasculopathy play a significant role in this process. Objectives This study aimed to investigate the effect of antioxidant α-tocopherol acetate and immunomodulator vitamin D 3 in the preclinical model of SSc. Methods To perform this study, three groups of laboratory animals were formed: a control group (20 animals), an experimental group #1 (25 animals) and an experimental group #2 (25 animals). Experimental animals were mature laboratory rats of the Wistar line weighing 180-220 g. Modeling of SSc was performed in the experimental group #1 using sodium hypochlorite (NaClO) according reported previously method [1]. Laboratory animals of the control group were injected with the isotonic solution according to the same scheme. In the experimental group #2, in addition to NaClO, laboratory rats received a solution of α-tocopherol acetate 10 mg / 100 g of body weight, IV and a solution of vitamin D3 1000 IU / 100 g of body weight, IV for 3 weeks (second half of the experiment). The level of surfactant protein D (Elabscience SP-D ELISA-Kit), the vascular cell adhesion molecule (Elabscience VCAM-1 ELISA-Kit), and interleukin 13 (Elabscience IL-13 ELISA-Kit) was determined by enzyme-linked immunosorbent assay. ELISA was performed on an enzyme-linked immunosorbent assay STAT FAX 303 plus. Data distribution was evaluated with the Shapiro-Wilk test. Mann-Whitney U test and unpaired t test were used for comparisons between groups, a p-value &lt;0.05 was considered statistically significant. Results The level of IL-13 (pg/ml) in the experimental group was higher than in the control 136,4± 56,23 vs 9,43± 4,01 (p&lt;0.001). The serum concentration of SP-D (pg/ml) among the experimental group of laboratory animals was significantly higher compared to the control subjects 490,20 [228,75–568,73] vs 70,13±33,21, (p&lt;0,05). VCAM-1(pg/ml) was also higher in the experimental group VCAM-1 91,25 [85,63–143,75] vs 17,35±7,47, (p&lt;0,05). The administration of vitamin D3 and α-tocopherol acetate has shown a positive effect for all three investigated parameters. We have found a statistical difference between the two experimental groups regarding IL-13, SP-D, and VCAM-1. The SP-D level in the experimental group #2 was 124.93 ± 18.96, which was significantly lower than in the experimental group #1 (p=0.04). The concentration of IL-13 and VCAM were also lower in the group of vitamins D3 and α-tocopherol acetate administration (22.88±5.11, p&lt;0.001 and 38.73±12.13, p=0.02 respectively). Conclusion This study provided evidence that administration of vitamins D3 and α-tocopherol acetate, given in combination, has a beneficial effect on IL-13, SP-D, and VCAM-1 levels in the organism of the experimental animals. References [1]Doskaliuk B, Zaiats L, Yatsyshyn R. AB0090 The biochemical basis of animal model of systemic sclerosis. Annals of the Rheumatic Diseases 2021;80:1074. http://dx.doi.org/10.1136/annrheumdis-2021-eular.732 Disclosure of Interests None declared.