AB0078 NESFATIN-1 EXPRESSION ASSOCIATED WITH A MARKER FOR BONE MATRIX FORMATION P1NP IN RHEUMATOID ARTHRITIS
Authors: L. Seewordova, J. Polyakova, E. Papichev, Y. Akhverdyan, В. Zavodovsky
Journal: Annals of the Rheumatic Diseases (2022)

Background Currently, the role of tissue cytokines in the pathogenesis of various diseases is being actively studied [1,2]. Nesfatin-1 (NF-1) is an endogenous peptide with pleiotropic activity. Recently, there have been works on the involvement of NF-1 in osteogenesis. In the experiment, intravenous administration of NF-1 to ovariectomized rats (OVX) leads to an increase in bone mineral density (BMD). There are works showing inhibition of osteoclastogenesis in mice [3,4]. We assume that the level of NF-1 affects the processes of bone matrix formation. The aim of the study was to determine the relationship between the level of NF-1, bone mineral density, composite body composition and markers of bone metabolism in patients with rheumatoid arthritis (RA). Objectives The study randomized 110 patients with RA according to the ACR/EULAR criteria, 2010 and 30 people in the control group. Methods All of them underwent osteodensitometry LUNAR DPX-Pro with the determination of composite body composition (BCT) and bone mineral density (BMD). NF-1 levels and bone turnover markers were determined using a commercial ELISA test. Results The average concentration of NF-1 in patients with RA was 50.49±34.05 ng/ml, which is significantly higher than in healthy individuals. The average level of NF-1 in the blood serum of healthy individuals was 31.61±3.17 ng/ml (M±σ). The level of reference indicators of NF-1 in healthy individuals, defined as М±2σ, ranged from 25.27 to 37.95 ng/ml. According to the level of NF-1, all patients with RA were divided into 2 subgroups. The 1st group included patients (n=44) with normal serum NF-1 concentration (less than 37.95 ng/ml), the 2nd group (n=66) included patients with elevated NF-1 levels (more than ng/ml). During the analysis of the results of the study, we revealed a statistically significant correlation between NF-1 and the N-terminal propeptide of type I procollagen (P1NP)) (r = 0.218, p = 0.022). The level of CRP and the erythrocyte sedimentation rate positively correlated with the indicators of NF-1. However, we found no relationship between NF-1 levels and BMD; composite body composition; as well as the level of C-terminal type I collagen telopeptide (β-CTx). Conclusion Thus, the relationship between nesfatin-1 and a marker of bone matrix formation (P1NP) was revealed. This may indicate a possible effect of nesfatin-1 on the differentiation and function of osteoblasts. References [1]Kvlividze T. Z., Zavodovsky B. V., Akhverdyan Y. R., Sivordova (Seewordova) L. E., Polyakova Yu.V., Lavrov D.P., A.T. Yakovlev. Elevated Nesfatin-1 levels in patients with Rheumatoid arthritis. Annals of the Rheumatic Diseases. 2018; DOI: 10.1136/annrheumdis-2018-eular.4459 (In Russ.). [2]Polyakova YV, Zavodovsky BV, Sivordova LE, Akhverdyan YR, Zborovskaya IA. Visfatin and Rheumatoid Arthritis: Pathogenetic Implications and Clinical Utility. Curr Rheumatol Rev. 2020;16(3):224-239. doi: 10.2174/1573397115666190409112621. PMID: 30963978. [3]Chen D., Cao S., Chang B., et al. Increasing hypothalamic nucleobindin 2 levels and decreasing hypothalamic inflammation in obese male mice via diet and exercise alleviate obesity-associated hypogonadism. neuropeptides. 2019 Apr;74:34-43. doi: 10.1016/j.npep.2018.10.005. Epub 2018 Nov 1. [4]Li R., Wu Q., Zhao Y., et. Al. The novel pro-osteogenic activity of NUCB2(1-83). PLoS One. 2013. (4):e61619. Disclosure of Interests None declared