tbuckley/cabot-search huggingface dataset:
AB0136 INTERFERON STIMULATED GENES IN CD4 T CELLS ARE ASSOCIATED WITH PYROPTOSIS PATHWAYS IN PATIENTS WITH LUPUS
Authors: S. J. Lee, B. A. Kim
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Single cell RNA sequencing (scRNA-seq) of kidney tissues in patients with lupus has led to landscape of complete cellular composition and states for immune and non-immune cells. These studies suggested type I interferon (IFN) signatures prime inflammatory responses. However, there is a lack of knowledge regarding the characteristics of subsets of IFN stimulating genes (ISG) high expressed cells. <h3>Objectives</h3> Here we investigated immune cell compositions between peripheral blood mononuclear cells (PBMCs), skin and kidney tissues in patients with lupus using scRNA-seq. We characterized a subset of ISG high CD4 T cells, which are commonly expressed across PBMCs, skins and kidneys of lupus but not skins of healthy. <h3>Methods</h3> scRNA-seq dataset of PBMCs, skin and kidney tissues in patients with lupus were collected. We integrated and analyzed immune cell compositions from this scRNA-seq dataset. We focused on subset of ISG high expressed CD4 T cells and characterized top 200 genes of this subset. <h3>Results</h3> There is one subset of ISG high expressed CD4 T cells in the lupus PBMCs, kidneys, and skins but not in the healthy skins. This ISG high expressed CD4 T cells have the greatest number of type I interferon signatures and gasdermin D gene, which is related to pyroptosis of cells, among top 200 genes. We further investigated pyroptosis pathway genes in the subsets of CD3 T cells. Interestingly, the subset of ISG high expressed CD4 T cells have most increased pyroptosis related upstream regulating genes including IRF1, GBP1, CASP4 and CASP1. Furthermore, this subset highly expressed inflammasome gene such as NLRC5 not NLRP3 <h3>Conclusion</h3> There are ISG high expressed CD4 T cells across tissues of lupus. Those cells highly expressed pyroptosis pathway related genes. Further investigation is needed to characterize association between ISG and pyroptosis. <h3>Reference</h3> [1]Arazi A, Rao DA, Berthier CC, Davidson A, Liu Y, Hoover PJ, et al. The immune cell landscape in kidneys of patients with lupus nephritis. Nat Immunol 2019;20:902-14. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
POS1346 CHASING PAIN: EXPLORING SOMATOSENSORY PROFILES IN PATIENTS WITH RHEUMATOLOGICAL DISEASES THROUGH QUANTITATIVE SENSORY TESTING
Authors: C. J. Bauer, R. Schrapper, S. Petzinna, C. Behning, T. Bender, P. Brossart, M. Mücke, V. Schäfer
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Pain reflects a major symptom in many rheumatological diseases. To assess nociceptive and non-nociceptive submodalities of different afferent nerve fiber groups and central pathways, and to examine the presence of sensitive plus or minus signs such as hyperalgesia or hypesthesia, the "Quantitative Sensory Testing" (QST) approach was developed. QST, established by the German Neuropathic Pain Research Network (DFNS), is meant to be used for the generation of somatosensory profiles [1]. These can then be used as a guidance to improve a targeted pain medication. <h3>Objectives</h3> The goal of this study is to explore whether patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), axial spondyloarthritis (axSpA) and systemic sclerosis (SSc) show differences in somatosensory profiles between each other and in contrast to a healthy control group. <h3>Methods</h3> In this monocentric five-armed study (ethical approval under Institutional Review Board #065/20), four groups of each 20 patients with RA, PsA, axSpA or SSc, and one control group consisting of 20 healthy individuals were collected and a somatosensory profile using the standardized procedure of QST was created for each one of the 100 participants. QST included both small fiber mediated stimuli and large fiber mediated stimuli, via all categories shown in Figure 1. The vibration detection threshold where the detection of vibration is ranked on a scale from zero to eight, with '8' being normal perception of vibration, serves as an example for large fiber mediated stimuli. Additionally, standard questionnaires incorporating laboratory parameters, joint manifestations and pain condition were used to determine disease activities (BASDAI, PASDAS, CDAI and mRSS). <h3>Results</h3> A preliminary data analysis of all 100 study participants found occurrence of allodynia in 5% of patients with SSc, 15% of RA, 25% of PsA, and 15% of all axSpA patients, compared with 0% in the control group. Considering the vibration detection threshold, there was little difference between all disease groups, also in comparison to the control group: SSc (mean ± SD: 7,79 ± 0,34), RA (mean ± SD: 7, 66± 0,4), PsA (mean ± SD: 7,76 ± 0,34), AxSpA (mean ± SD: 7,69 ± 0,57) and control group (mean ± SD: 7,98 ± 0,16). <h3>Conclusion</h3> The analysis for allodynia occurrence indicates the presence of sensory gain towards small fiber mediated stimuli in all four disease groups studied. Until now, our vibration detection threshold studies do not suggest a loss or gain of sensory function for large fiber mediated stimuli. The full analysis completion is expected in March 2023. <h3>Reference</h3> [1]R.Rolke et al. Quantitative sensory testing: a comprehensive protocol for clinical trials, European Journal of Pain 10,2006; p. 77-88 <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB1037 ARE THE CLINICAL SCALES USED TO ASSESS DISEASE ACTIVITY AND FUNCTIONAL ABILITY IN AXIAL SPONDYLOARTHROPATHY REALLY RELIABLE?
Authors: M. A. Mortada, N. Ehab, S. Elnaggar, E. Elshahawy
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Evaluation of disease activity and functional impairment in Axial spondyloarthritis (AxSpA) are important in therapeutic plan. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Ankylosing Spondylitis Disease Activity Score (ASDAS) and The Bath Ankylosing Spondylitis Functional Index (BASFI) scores are based largely on subjective measures, which liable to change depending on patient's expression, culture, and awareness. <h3>Objectives</h3> Assessment of reliability of BASFAI, BASDAI, ASDAS-ESR, and ASDAS-CRP as a total score and individual questions in patients with AxSpA. <h3>Methods</h3> This cross-sectional study was conducted on 103 patients with AxSpA according to the ASAS classification criteria for AxSpA. Each patient completed BASFAI, BASDAI, ASDAS-ESR, and ASDAS-CRP during their routine visit for follow up with one rheumatologist. Then the same patients completed the three questionnaires again in the same day or on the second day with another rheumatologist. <h3>Results</h3> Internal consistency and reliability of ASDAS-ESR, ASDAS-CRP, BASDAI, and BASFAI scores was good (ICC was 0.841, 0.820, 0.767, and 0.852 respectively). Reliability of BASFAI score was better than that of ASDAS-ESR, ASDAS-CRP, BASDAI scores, and that of ASDAS-ESR, ASDAS-CRP was better than reliability of BASDAI score. <h3>Conclusion</h3> Some questions of ASDAS, BASDAI, and BASFAI scores are more reliable than others, this depends on the question. The answers of the questions that assess sensation of pain, are liable to change. While the answers of other questions that assess stiffness or assess its duration are less liable to change. Questions that assess certain daily activity are more reliable than that assess the ability to do more than one activity. <h3>REFERENCES:</h3> NIL. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB0391 THE IMPACT OF OBESITY ON LIPID PROFILE AND ATHEROGENIC INDEXES AMONG RHEUMATOID ARTHRITIS ELDERLY PATIENTS
Authors: M. Ardhaoui, M. Ghali, J. Mahbouba, S. Zrour, I. Bejia, M. Touzi, N. Bergaoui
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Obesity and dyslipidemia are two common comorbidities in rheumatoid arthritis (RA). They are considered as important cardiovascular and atherosclerosis risk factors in RA. <h3>Objectives</h3> The aim of our study was to evaluate the impact of obesity on lipid parameters as well as on atherogenic indexes among RA elderly patients. <h3>Methods</h3> A cross-sectional study was conducted including patients followed for RA and aged ≥ 65 years. All patients had lipid measurements of standard (total cholesterol (TC), high-density lipoproteins (HDL), low-density lipoproteins (LDL), and triglycerides (TG)) and specific (Apolipoproteins (Apo) A1 and B) parameters. We calculated the atherogenic indexes TC/HDL and LDL/HDL and apoB/apoA1. Patients were divided according to body mass index (BMI), into 2 groups: G1=obese patients with BMI ≥ 30 Kg/m² and G2 of non obese patients with BMI &lt;30. <h3>Results</h3> We included 63 patients (52 women and 11 men). The mean age was 68.17±4.35 years [65-83]. The median duration of disease was 11 years. The mean BMI was 27.97±5.44 kg/m2 [14.85-43.2]. Twenty-two patients (34.9%) were obese (G1) and 41 patients (65.1%) had a BMI &lt; 30 (G2). Mean levels of Apo A1, Apo B, TC, HDL, LDL and TG were 1.53±0.34 g/L, 1.1±0.29 g/L, 4.91±1.03 mmol/L, 1.09±0.29 mmol/L, 2.96±0.86 mmol/L and 2.02±1.47 mmol/L, respectively. Mean values of the atherogenic indexes TC/HDL, LDL/HDL and ApoB/ApoA1 were 4.77±1.59, 2.96±1.39 and 0.74±0.2 respectively. Comparing the G1 of obese patients with the G2, we found that obese patients had changes in lipid profile with significantly higher levels of Apo B (1.22±0.29 g/L vs. 1.04±0.27; p=0.018), TC (5.6±0.82 mmol/L vs 4.2±1.07; p=0.008) and LDL (3.5±0.84 mmol/L vs 2.54±0.84; p=0.03). A significantly lower ApoA1 level was noted in G1 (1.47±0.3 g/L vs 1.66±0.24; p=0.019). A lower level of HDL was noted in the G1 (1.06±0.33 mmol/L vs 1.15±0.2), but without significant association (p=0.5). However, there was no significant difference in TG levels (p=0.9) and atherogenic indexes including TC/HDL (p=0.07), LDL/HDL (p=0.1) and ApoB/ApoA1 (p=0.2). <h3>Conclusion</h3> We demonstrated that obesity is associated with alterations in many lipid parameters among RA elderly patients. Obesity actually acts in synergy with the various conventional factors, including dyslipidemia, to increase the cardiovascular risk in this population. <h3>REFERENCES:</h3> NIL. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB1070 CERVICAL SPINE INVOLVEMENT IN AXIAL SPONDYLOARTHRITIS. RADIOGRAPHIC CHARACTERISTICS AND ASSOCIATED FACTORS
Authors: L. Berbel Arcobé, X. Michelena, J. A. Narvaez, J. M. Nolla, J. Palacios Olid, P. Maymo Paituvi, X. Juanola-Roura
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> The study of the cervical spine (CS) in axial spondyloarthritis (axSpA) and its radiographic characteristics including the zygapophyseal joints (ZJ) may be helpful for early diagnosis, establishing a prognosis and sharpening treatment choices. <h3>Objectives</h3> To describe the prevalence and characteristics of CS involvement in a cohort of patients with axSpA and its associated factors, who have been followed up in the Rheumatology Department of a third level university hospital. <h3>Methods</h3> Descriptive, retrospective and unicentric study. Patients with an axSpA diagnosis based on the ASAS classification criteria, from March 2011 to December 2022, retrieved from a dedicated axSpA database were included. Sociodemographic, clinical, radiographic and therapeutic variables were gathered. They were separated into two groups according to CS involvement; "CS group" as patients with BASRI≥2 in CS and/or a score ≥3 in ZJ measured by De Vlam method<sup>[1]</sup>; and "no CS group" as controls. <h3>Results</h3> A total of 340 patients were included (71.8% men; mean age 57,4 ± 14,9 years). CS involvement is common among axSpA (n=182; 53,5%). ZJ involvement was observed in 99 patients (29,1%), and of those, 21 did not show concomitant structural damage in the vertebral bodies. A total of 83 patients (24,4%) had damage in the vertebral bodies exclusively, and 37 (10,9%) had CS involvement without radiographic involvement of the lumbar spine (LS) (29,7% women). Patients from the CS group were predominantly men, older, had a higher BMI and higher prevalence of smoking (Table 1). They also showed higher disease activity (BASDAI and ASDAS CRP), worse function (BASFI), worse mobility in CS and LS, as well as more structural damage in both LS and sacroiliac joints. No differences were found between groups for positive HLA B27, age at diagnosis, familiar history, presence of arthritis, enthesitis, dactylitis or extra musculoskeletal manifestations (uveitis, psoriasis or IBD). <h3>Conclusion</h3> The radiographic evaluation of the CS is relevant in axSpA, and it should be performed routinely, as well as the evaluation of the ZJ, as they are commonly involved in axSpA and related to higher disease activity and worse function. <h3>Reference</h3> [1]De Vlam K, Mîelants H, Veys EM. Involvement of the zygapophyseal joint in ankylosing spondylitis: relation to the bridging syndesmophyte. J Rheumatol 1999;26:1738-45. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB1817-HPR CROSS-CULTURAL ADAPTATION AND VALIDATION OF THE TURKISH VERSION OF THE NORWICH PATELLAR INSTABILITY SCORE IN PATIENTS WITH PATELLAR INSTABILITY
Authors: I. Ayas, Z. Hazar, I. Kaya, M. Ataoglu, U. Kanatli
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> The Norwich Patellar Instability Score (NPIs) score is a tool for evaluating the impact of patellofemoral instability on joint function. It has not been translated or culturally adapted for the Turkish population before. <h3>Objectives</h3> The aim of this study was to translate and validate the NPIs in Turkish patients with patellar instability. <h3>Methods</h3> Sixty-four individuals with patellar instability were included in this cross-sectional study. Test-retest reliability of the Turkish version of NPIs was assessed by the intraclass correlation coefficient (ICC) and Cronbach's alpha for internal consistency. For construct validity, correlation coefficients between the Turkish version of NPIs developed by the translation-back translation method, Kujala patellofemoral disorder score, and Lysholm knee score were analyzed. <h3>Results</h3> The Turkish version of the NPI score showed high internal consistency (Cronbach's alpha = 0.97) and excellent test-retest reliability (ICC<sub>2,1</sub> = 0.90). The Turkish version of the NPI score had a strong correlation with Kujala patellofemoral disorder score (Rp = -0.85, p&lt; 0.001) and the Lysholm knee score (Rp = -0.89, p&lt; 0.001). A floor effect was observed in the present study and there was no ceiling effect. <h3>Conclusion</h3> The Turkish version of the NPIs is a reliable and valid tool in patients with patellar instability. The Turkish version of the NPIs will be a guide for clinicians and researchers to understand the functional status of patients with patellar instability. <h3>References</h3> [1] Smith, T.O., et al., <i>The development, validation, and internal consistency of the Norwich Patellar Instability (NPI) score.</i> Knee Surgery, Sports Traumatology, Arthroscopy, 2014. 22(2): p. 324-335. [2] Beaton, D.E., et al., <i>Guidelines for the process of cross-cultural adaptation of self-report measures.</i> Spine, 2000. 25(24): p. 3186-3191. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB0627 CLINICAL PROFILE OF PEDIATRIC SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS IN A TERTIARY LEVEL HOSPITAL IN THE PHILIPPINES, YEAR 2015-2021
Authors: J. Garcia
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Pediatric Lupus Erythematosus is a chronic systemic autoimmune disease with a higher risk of disease damage than adults with SLE. <h3>Objectives</h3> To profile the clinical and laboratory features and manifestations of pediatric SLE patients seen and admitted at Region 1 Medical Center from January 2015 to December 2021 and assess the association of the clinical profile with complications and mortality. <h3>Methods</h3> A retrospective review of medical records of all pediatric SLE patients age &lt;18 years, admitted at Region 1 Medical Center from January 2015 to December 2021. Charts were reviewed for clinical and laboratory data extraction. Clinical data were analyzed using descriptive statistical analysis. Fisher's exact test was utilized to analyze the association of clinical profile to morbidity and mortality. Odds ratio was used to determine the degree and direction of association among significantly associated variables in the study. <h3>Results</h3> A total of 29 patients who fulfilled the inclusion criteria were included in the study. Average age of diagnosis is 16 years with predominance of female. The most common presenting symptom is malar rash(27.6%) followed by joint pains (10.3%) and pallor (10.3%). Patients with pediatric SLE commonly manifested with acute cutaneous rash(79.3%). Thrombocytopenia (69%) is the most common laboratory finding. Complications of SLE include renal (51.7), infection (27.6%), and cardiovascular (17.2%). Oral corticosteroid is the most common treatment which was administered to all participants. Mortality rate of 34.5% is associated to the most common cause which is SLE in activity (80%) followed by infection (60%). Neurologic manifestation and increase creatinine were found to be associated with mortality with odds ratio of &gt;1. <h3>Conclusion</h3> Malar rash is the most common clinical presentation among Filipino pediatric SLE. The most common complication is renal involvement. Neurologic manifestation and non-scarring alopecia increase mortality. <h3>Reference</h3> [1]PubMed Central, ResearchGate, Journals, World Health Organization website <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB1374 ADVERSE EVENTS AFTER VACCINATION FOR SARS-CoV2 IN A CASE SERIES OF FIBROMYALGIA PATIENTS VERSUS HEALTHY CONTROLS
Authors: M. L. Aprile, L. Cutore, D. Sambataro, G. Sambataro, M. Colaci
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Fibromyalgia (FM) is a chronic widespread pain syndrome of unknown origin that leads to hypersensitivity for physical, chemical and/or psychic triggers. Vaccination, as an inflammatory stimulus and as a psychologically stressful act, could represent a challenge for these patients. <h3>Objectives</h3> We aimed to investigate the incidence of adverse reactions after vaccination for Sars-Cov2 in a series of FM patients versus healthy controls. <h3>Methods</h3> We recruited 65 consecutive FM patients classified according to the 2016 ACR diagnostic criteria¹ (M/F: 5/60; mean age 53.6 +/-12.5 years), without other associated rheumatologic conditions, and 65 age/sex-matched healthy controls. All patients filled a questionnaire in order to investigate eventual adverse events occurring up to 6 months after administration of a Sars-Cov2 vaccine. The questionnaire was divided into two parts: the first part included the patient's demographic information, the vaccine type performed and the anamnestic data. In the second part, the individuals described all new symptoms or signs occurred after the first, the second or the third dose of Sars-Cov2 vaccine. <h3>Results</h3> Overall, FM patients reported a higher frequency of adverse events after Sars-Cov2 vaccination in comparison with healthy controls. In particular, 44/65 FM patients vs. 11/65 controls complained of exacerbation of diffuse pain (p&lt;0.001). Fatigue, diarrhea, sweating, tingles, headache, dizziness, transient respiratory discomfort, and paroxysmal vision blurring were also more frequent in FM patients than controls (47/65 vs. 30/65, p=0.004; 6/65 vs. 0/65, p=0.028; 18/65 vs. 8/65, p=0.047; 20/65 vs. 0/65, p&lt;0.001; 22/65 vs. 9/65, p=0.013; 21/65 vs. 5/65, p&lt;0.001; 10/65 vs 1/65, p=0.009; 17/65 vs. 2/65, p&lt; 0.001, respectively). No significant difference between FM and the control group as regards fever was reported (24/65 vs. 30/65; p=0.7). Interestingly, swelling at the injection vaccine site was more commonly reported in controls (9/65 vs. 20/65; p=0.034). Finally, one case of Bell's palsy was registered in the FM series while one case of myocarditis in the control group. <h3>Conclusion</h3> FM patients showed an increased frequency of adverse events to Sars-Cov2 vaccination compared to healthy controls. In particular, all the symptoms reported seemed to be associated with the functional hypersensitivity that characterizes FM. <h3>Reference</h3> [1]Wolfe F, et Al. <i>2016 Revisions to the 2010/2011 fibromyalgia diagnostic criteria</i>. Semin Arthritis Rheum. 2016 Dec; 46(3):319-329. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.
AB0043 SERIAL ADMINISTRATION OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 AND OSTEOPROTEGERIN-FC ENHANCES THE DIFFERENTIATION OF OSTEOBLASTS
Authors: C. N. Son, H. J. Choi, J. M. Kim, S. H. Kim
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Osteoprotegerin (OPG) is an intrinsic antagonist of the RANKL. A recent study reported that RANKL reverse signaling in osteoblasts may prepare osteoblasts for further maturation and that vesicular RANK stimulates osteoblast differentiation. Hence, OPG is expected to accelerate osteoblastogenesis by affecting RANKL reverse signaling. <h3>Objectives</h3> We compared the level of differentiation from preosteoblasts to osteoblasts by serially administrating recombinant human bone morphogenetic protein-2 (rhBMP-2), involved in bone formation and regeneration, and osteoprotegerin-immunoglobulin Fc segment complex (OPG-Fc), an osteoclast inhibitor. <h3>Methods</h3> The MC3T3-E1 preosteoblast cell line was differentiated for one, three, and seven days with a treatment of OPG-Fc in 10–200 ng/mL concentration and the cell viability was evaluated by Cell Counting Kit-8 analysis. The level of differentiation from MC3T3-E1 cells to osteoblasts was determined by alkaline phosphatase activity. The level of runt domain-containing transcription factor 2 (Runx2) and osteopontin (OPN) manifestation, involved in osteoblast differentiation, was examined by real-time polymerase chain reaction and western blotting. <h3>Results</h3> During MC3T3-E1 cell differentiation, the differentiation level was high with one-day treatment using 100 ng/mL OPG-Fc. The treatment with 50 ng/mL rhBMP-2 for seven days, followed by one-day treatment with 100 ng/mL OPG-Fc produced the highest differentiation level, which was approximately 5.3 times that of the control group (P &lt; 0.05). The expression of Runx2 mRNA significantly increased, reaching 2.5 times the level of the control group under the condition of seven-day treatment with rhBMP-2 and one-day treatment with OPG-Fc (P &lt; 0.001). The expression of Runx2 protein significantly increased to approximately 5.7 times that of the control group under the condition of seven-day treatment with rhBMP-2, followed by one-day treatment with OPG-Fc (P &lt; 0.01). The expression of OPN protein showed no change from that of the control group under various conditions of rhBMP-2 and OPG-Fc combinations. <h3>Conclusion</h3> Differentiation ability of preosteoblasts to osteoblasts was strong with serial treatment and rhBMP-2, followed by OPG-Fc. Runx2 and OPN mRNA levels and Runx2 protein levels increased. These results imply that the combination of OPG-Fc and rhBMP-2 increased osteoblast differentiation efficacy. <h3>Reference</h3> [1]Ikebuchi Y, Aoki S, Honma M, et al. (2018) Coupling of bone resorption and formation by RANKL reverse signalling. Nature. 561, 195-200. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> None Declared.