tbuckley/cabot-search huggingface dataset:
Adult human kidney organoids originate from CD24+ cells and represent an advanced model for adult polycystic kidney disease
Authors: Yaoxian Xu, Christoph Kuppe, Javier Perales-Patón, Sikander Hayat, Jennifer Kranz, Ali T. Abdallah, James Nagai, Zhijian Li, Fabian Peisker, Turgay Saritas, Maurice Halder, Sylvia Menzel, Konrad Hoeft, Annegien Kenter, Hyojin Kim, Claudia R. C. van Roeyen, Michael Lehrke, Julia Moellmann, Thimoteus Speer, Eva M. Buhl, Remco Hoogenboezem, Peter Boor, Jitske Jansen, Cordula Knopp, Ingo Kurth, Bart Smeets, Eric Bindels, Marlies E. J. Reinders, Carla Baan, Joost Gribnau, Ewout J. Hoorn, Joachim Steffens, Tobias B. Huber, Ivan Costa, Jürgen Floege, Rebekka K. Schneider, Julio Saez-Rodriguez, Benjamin S. Freedman, Rafael Kramann
Journal: Nature Genetics (2022)
Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease
Authors: Xie Li, Yinan Zhang, Lingyan Xu, Aoxue Wang, Yejun Zou, Ting Li, Li Huang, Weicai Chen, Shuning Liu, Kun Jiang, Xiuze Zhang, Dongmei Wang, Lijuan Zhang, Zhuo Zhang, Zeyi Zhang, Xianjun Chen, Wei Jia, Aihua Zhao, Xinfeng Yan, Haimeng Zhou, Linyong Zhu, Xinran Ma, Zhenyu Ju, Weiping Jia, Congrong Wang, Joseph Loscalzo, Yi Yang, Yuzheng Zhao
Journal: Cell Metabolism (2022)
The Toxoplasma effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages
Authors: Arne L. ten Hoeve, Laurence Braun, Matias E. Rodriguez, Gabriela C. Olivera, Alexandre Bougdour, Lucid Belmudes, Yohann Couté, Jeroen P.J. Saeij, Mohamed-Ali Hakimi, Antonio Barragan
Journal: Cell Host & Microbe (2022)

Upon pathogen detection, macrophages normally stay sessile in tissues while dendritic cells (DCs) migrate to secondary lymphoid tissues. The obligate intracellular protozoan Toxoplasma gondii exploits the trafficking of mononuclear phagocytes for dissemination via unclear mechanisms. We report that, upon T. gondii infection, macrophages initiate the expression of transcription factors normally attributed to DCs, upregulate CCR7 expression with a chemotactic response, and perform systemic migration when adoptively transferred into mice. We show that parasite effector GRA28, released by the MYR1 secretory pathway, cooperates with host chromatin remodelers in the host cell nucleus to drive the chemotactic migration of parasitized macrophages. During in vivo challenge studies, bone marrow-derived macrophages infected with wild-type T. gondii outcompeted those challenged with MYR1- or GRA28-deficient strains in migrating and reaching secondary organs. This work reveals how an intracellular parasite hijacks chemotaxis in phagocytes and highlights a remarkable migratory plasticity in differentiated cells of the mononuclear phagocyte system.
Common features of neurodegenerative disease: exploring the brain-eye connection and beyond (Part 1): the 2021 pre-symposium of the 15th international conference on Alzheimer’s and Parkinson’s diseases
Authors: Sharyn L. Rossi, Preeti Subramanian, Guojun Bu, Adriana Di Polo, Todd E. Golde, Diane E. Bovenkamp
Journal: Molecular Neurodegeneration (2022)

share similar mechanisms of neurodegeneration across multiple diseases.This meeting report (Part 1 of 2) summarizes two of the hot topics discussed in the AD/PD 2021 Common Features, including cellular senescence and resilience/resistance (visit the meeting website for a complete agenda https://adpd2021.kenes.com/commonfeatures-of-neurodegenerative-disease-exploring-thebrain-eye-connection-and-beyond).As funders of brain and eye diseases, BrightFocus supports research in each of these critical areas of disease pathology and anticipates that scientific discussions on these common elements will catalyze cross-disease collaborations and breakthroughs (information on how to apply for funding can be found at science.brightfocus.org/apply-grant).Since the topics and speakers change at every meeting, we look forward to your participation in the fourth installment of Common BrightFocus Foundation sponsored the third iteration of the "Common Features of Neurodegenerative Disease: Exploring the Brain-Eye Connection and Beyond" preconference symposium which has preceded the bi-annual International Conference on Alzheimer's and Parkinson's Diseases (AD/PD) for the past six years.Common Features highlights areas of disease pathology that Molecular Neurodegeneration
Cost effectiveness of clozapine in neuroleptic-resistant schizophrenia
Authors: H Y Meltzer, P Cola, L Way, P A Thompson, B Bastani, M A Davies, B Snitz
Journal: American Journal of Psychiatry (1993)

The goal of this study was to determine whether clozapine is a cost-effective treatment for treatment-resistant schizophrenia.Data were collected on 96 treatment-resistant patients with schizophrenia for 2 years before they entered a clozapine treatment study and for at least 2 years after they entered the study. Information about the cost of inpatient and outpatient treatment, housing costs, other costs, and family burden through direct interview or questionnaire of these patients and their families were available for 47 of the 96 patients. Data on lost income and Social Security disability insurance were also obtained. Outcome measures included psychopathology, quality of life, global functioning, work function, and rehospitalization.The cost of treatment was significantly decreased in the patients who continued clozapine treatment for at least 2 years. This was primarily due to a dramatic decrease in the frequency and cost of rehospitalization. Costs were nonsignificantly lower in patients who dropped out of treatment. The estimated total 2-year cost for the 59 patients who continued clozapine treatment, the 34 patients who dropped out, and the three who interrupted treatment decreased from $7,390,206 to $5,719,463, a savings of $8,702/year per patient. There was a decrease in total costs of $22,936/year for the 37 patients who continued clozapine treatment for whom cost data were available. There were no significant changes in lost income or Social Security disability insurance payments in either group. Clozapine produced a marked improvement in Brief Psychiatric Rating Scale total scores as well as positive negative symptom scores, Global Assessment Scale scores, Quality of Life Scale scores, work functioning, capacity for independent living, and rehospitalization rates.Clozapine is a cost-effective treatment for treatment-resistant schizophrenic patients. Cost savings result almost exclusively from the reduced cost of hospitalization.
Edoxaban for Thromboembolism Prevention in Pediatric Patients With Cardiac Disease
Authors: Michael A. Portman, Jeffrey P. Jacobs, Jane W. Newburger, Felix Berger, Michael A. Grosso, Anil Duggal, Ben Tao, Neil A. Goldenberg, Matthew Brothers, Bradley Marino, Charles Canter, Mark Law, Nguyenvu Nguyen, Charlie Sang, Kristin Shimano, Dipankar Gupta, Michael Portman, Derek Williams, Lauren Glass, Charles Sperrazza, Steven Herold, Ruchira Garg, Mark Vranicar, Sawsan Awad, Alfred Asante-Korang, Colleen Druzgal, Caroline Ozment, Kamill Del Toro, Ferran Roses, Christian Jux, Verena Gravenhorst, Ulrich Schweigmann, Mihir Bhatt, Christine Sabapathy, Nagib Dahdah, Dototea Bartonicek, Gerald Tulzer, Elena Basargina, Tatiana Zvereva, Tatiana Pertels, Irina Plotnikova, S.E.G.U.E.L.A. Pierre-Emmanuel, Pascal Amedro, Dulac Yves, Damien BONNET, Paola Saraco, Alessandro Rimini, Valerii Digtiar, Margaryta Gonchar, Tetyana Kryuchko, Olga Yablon, Varinder Singh Bedi, Jashvant Patel, Monjori Mitra, Jacek Kusa, Kowalczyk Domagala, László KÖRNYEI, Csaba BERECZKI, László ABLONCZY, Vivianne Aviva Levitas, David Mishali, Shoshana Revel-Vilk, Dan Harlev, Hatice Ilgen Sasmaz, Namik Yasar Ozbek, Sule Unal, Türkan Patıroglu, Baris Malbora, Hasan Agin, Zeynep Karakas, Ramazan Kaan Kavakli, Elizabeth Chalmers, Frances Bu'Lock, Piers Daubeney, Hala Hamza, Mohamed Badr, Mohsen Elalfy, Ahmed Mansour, Hoda Hassab, Ayman Sabry, Linda Daou, Fadi Bitar
Journal: Journal of the American College of Cardiology (2022)
Neighborhood Household Income and Trends in 30-Day Readmission for Patients With Heart Failure
Authors: Nijat Aliyev, Muhammad Usman Almani, Muhammad Qudrat-Ullah, Josephine Harrington, Gregg C. Fonarow, Javed Butler, Ambarish Pandey, Muhammad Shahzeb Khan, Stephen J. Greene
Journal: JACC Heart Failure (2022)
Elevated C-Reactive Protein Amplifies Association of Lipoprotein(a) With Cardiovascular Risk and Clinical Benefit of Alirocumab
Authors: Gregory G. Schwartz, Michael Szarek, Andreas Zeiher, Harvey D. White, J. Wouter Jukema, Robert A. Harrington, Shaun G. Goodman, Rafael Diaz, Vera Bittner, Deepak L. Bhatt, Ph. Gabriel Steg
Journal: Journal of the American College of Cardiology (2022)
Implementation of High-Sensitivity Cardiac Troponin Assays in the United States
Authors: Cian McCarthy, Shuang Li, Tracy Y. Wang, Inbar Raber, Yader Sandoval, Nathaniel R. Smilowitz, Jason H. Wasfy, Ambarish Pandey, James A. de Lemos, Michael C. Kontos, Fred S. Apple, Lori B. Daniels, L. Kristin Newby, Allan S. Jaffe, James L. Januzzi
Journal: Journal of the American College of Cardiology (2022)
Clinical Presentation and In-Hospital Trajectory of Heart Failure and Cardiogenic Shock
Authors: Jaime Hernandez-Montfort, Manreet Kanwar, Shashank S. Sinha, A. Reshad Garan, Vanessa Blumer, Rachna Kataria, Evan H. Whitehead, Michael Yin, Borui Li, Yijing Zhang, Katherine L. Thayer, Paulina Baca, Fatou Dieng, Neil M. Harwani, Maya Guglin, Jacob Abraham, Gavin Hickey, Sandeep Nathan, Detlef Wencker, Shelley Hall, Andrew Schwartzman, Wissam Khalife, Song Li, Claudius Mahr, Ju Kim, Esther Vorovich, Mohit Pahuja, Daniel Burkhoff, Navin K. Kapur
Journal: JACC Heart Failure (2022)
Direct Oral Anticoagulants vs Vitamin K Antagonists in Patients With Antiphospholipid Syndromes
Authors: Candrika D. Khairani, Antoine Bejjani, Gregory Piazza, David Jimenez, Manuel Monreal, Saurav Chatterjee, Vittorio Pengo, Scott C. Woller, Josefina Cortes-Hernandez, Jean M. Connors, Yogendra Kanthi, Harlan M. Krumholz, Saskia Middeldorp, Anna Falanga, Mary Cushman, Samuel Z. Goldhaber, David A. Garcia, Behnood Bikdeli
Journal: Journal of the American College of Cardiology (2022)
Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors
Authors: Debbie L. L. Ho, Stacey Lee, Jianyi Du, Jonathan D. Weiss, Tony Tam, Soham Sinha, Danielle Klinger, Sean Devine, Art Hamfeldt, Hope T. Leng, Jessica E. Herrmann, Mengdi He, Lee G. Fradkin, Tze Kai Tan, David Standish, Peter Tomasello, Donald Traul, Noushin Dianat, Rukmini Ladi, Quentin Vicard, Kishore Katikireddy, Mark A. Skylar‐Scott
Journal: Advanced Healthcare Materials (2022)

Combining the sustainable culture of billions of human cells and the bioprinting of wholly cellular bioinks offers a pathway toward organ-scale tissue engineering. Traditional 2D culture methods are not inherently scalable due to cost, space, and handling constraints. Here, the suspension culture of human induced pluripotent stem cell-derived aggregates (hAs) is optimized using an automated 250 mL stirred tank bioreactor system. Cell yield, aggregate morphology, and pluripotency marker expression are maintained over three serial passages in two distinct cell lines. Furthermore, it is demonstrated that the same optimized parameters can be scaled to an automated 1 L stirred tank bioreactor system. This 4-day culture results in a 16.6- to 20.4-fold expansion of cells, generating approximately 4 billion cells per vessel, while maintaining >94% expression of pluripotency markers. The pluripotent aggregates can be subsequently differentiated into derivatives of the three germ layers, including cardiac aggregates, and vascular, cortical and intestinal organoids. Finally, the aggregates are compacted into a wholly cellular bioink for rheological characterization and 3D bioprinting. The printed hAs are subsequently differentiated into neuronal and vascular tissue. This work demonstrates an optimized suspension culture-to-3D bioprinting pipeline that enables a sustainable approach to billion cell-scale organ engineering.
Building blocks of community positive health: the contribution of Kenyan communities
Authors: Claudia Robbiati, Chloe Wood, Winnie Chelagat, Lilian Koskey, Geoffrey Mwai, Iregi Mwenja, Aloyce Odhiambo, Hinjal Bhatia, Emma Ogden, Martha Gutteridge, Laura E R Peters, Des Tan, Geordan Shannon
Journal: The Lancet Planetary Health (2022)
Receipt of First and Second Doses of JYNNEOS Vaccine for Prevention of Monkeypox — United States, May 22–October 10, 2022
Authors: Jennifer L. Kriss, Peter M. Boersma, Emalee Martin, Kirsten Reed, Jennifer Adjemian, Nathaniel Smith, Rosalind J. Carter, Kathrine R. Tan, Arunkumar Srinivasan, Sunanda McGarvey, Jennifer McGehee, Danielle Henderson, Noah Aleshire, Adi V. Gundlapalli
Journal: MMWR Morbidity and Mortality Weekly Report (2022)

CDC based on a recipient identifier assigned by the reporting entity and the three-digit reporting entity code.† † The interval in days between first and second doses was calculated using date of administration for each dose.Analyses were conducted using SQL Server Management Studio (version 18; Microsoft) and SAS software (version 9.4; SAS Institute).This activity was reviewed by CDC and was conducted consistent with applicable federal law and CDC policy.§ § During May 22-October 10, 2022, a total of 931,155 JYNNEOS vaccine doses were administered and reported to CDC by U.S. jurisdictions, including 628,610 (67.5%) first doses, 301,770 (32.4%) second doses, and 775 (0.1%) third,
Coronary Artery Bypass Surgery Without Saphenous Vein Grafting
Authors: Alistair Royse, Justin Ren, Colin Royse, David H. Tian, Stephen Fremes, Mario Gaudino, Umberto Benedetto, Y. Joseph Woo, Andrew B. Goldstone, Piroze Davierwala, Michael Borger, Michael Vallely, Christopher M. Reid, Rodolfo Rocha, David Glineur, Juan Grau, Richard Shaw, Hugh Paterson, Doa El-Ansary, Stuart Boggett, Nilesh Srivastav, Zulfayandi Pawanis, David Canty, Rinaldo Bellomo
Journal: Journal of the American College of Cardiology (2022)

Approximately 95% of patients of any age undergoing contemporary, coronary bypass surgery will receive at least 1 saphenous vein graft (SVG). It is recognized that SVG will develop progressive and accelerated atherosclerosis, resulting in a stenosis, and in occlusion that occurs in 50% by 10 years postoperatively. For arterial conduits, there is little evidence of progressive failure as for SVG. Could avoidance of SVG (total arterial revascularization [TAR]) lead to a different late (>5 year) survival? A literature review of 23 studies (N = 100,314 matched patients) at a mean 8.8 years postoperative found reduced all-cause mortality for TAR (HR: 0.77; 95% CI: 0.71-0.84; P < 0.001). An expanded analysis with a new unpublished data set (N = 63,288 matched patients) was combined with the literature review (N = 127,565). It found reduced all-cause mortality for TAR (HR: 0.78; 95% CI: 0.72-0.85; P < 0.001). Additional Bayesian analysis found a very high probability of a TAR-associated reduction all-cause mortality.
Risk Factors for In-Hospital Cardiac Arrest in Patients With ST-Segment Elevation Myocardial Infarction
Authors: Wei Gong, Yan Yan, Xiao Wang, Wen Zheng, Sidney C. Smith, Gregg C. Fonarow, Louise Morgan, Jing Liu, Dong Zhao, Changsheng Ma, Yaling Han, Gilles Montalescot, Shaoping Nie
Journal: Journal of the American College of Cardiology (2022)
Cancer Risk following Residential Exposure to Airborne Polychlorinated Biphenyls: A Danish Register-Based Cohort Study
Authors: Laura Deen, Karin Sørig Hougaard, Alice Clark, Harald William Meyer, Marie Frederiksen, Lars Gunnarsen, Helle Vibeke Andersen, Thomas Hougaard, Kajsa Kirstine Ugelvig Petersen, Niels Erik Ebbehøj, Jens Peter Bonde, Sandra Søgaard Tøttenborg
Journal: Environmental Health Perspectives (2022)

Background: Polychlorinated biphenyls (PCBs) are biopersistent chemicals classified as human carcinogens. This classification is primarily based on evidence on higher-chlorinated PCBs found in food. The carcinogenic potential of airborne lower-chlorinated PCBs remains largely unexplored. Objectives: We aimed to investigate cancer risk following residential exposure to airborne PCBs. Methods: Cancer risk was examined in the Health Effects of PCBs in Indoor Air (HESPAIR) cohort of 38,613 residents of two partly PCB-contaminated residential areas in Greater Copenhagen, identified by nationwide registries. PCB exposure was based on relocation dates and indoor air PCB measurements in subsets of apartments. Cancer diagnoses were extracted from the Danish Cancer Registry for the follow-up period of 1970–2018. We estimated adjusted hazard ratios with time-varying cumulative exposure and a 10-y lag using Cox regression. Results: Overall risk of cancer was not associated with PCByear, [hazard ratio (HR) for high-exposed vs. low-exposed =0.98; 95% confidence interval (CI): 0.88, 1.09], but residents exposed to ≥3,000 ng/m3 PCB×year had higher risk of liver cancer (HR =2.81; 95% CI: 1.28, 6.15) and meningiomas (HR =3.49; 95% CI: 1.84, 6.64), with indications of exposure–response relationships. Results were suggestive of a higher risk of pancreatic cancer (HR =1.59; 95% CI: 0.95, 2.64) at the highest aggregated PCB level. For testis cancer, a higher risk was observed among residents exposed to 300–949 ng/m3 PCB×year relative to residents exposed to <300 ng/m3 PCB×year (HR =2.97; 95% CI: 1.41, 6.28), but the risk was not higher for residents exposed to ≥950 ng/m3 PCB×year. Apart from this, the risk of specific cancers was similar across exposure groups. Discussion: In this, to our knowledge, first population-based cohort study of residential exposure to airborne PCBs, we found no association between exposure to PCBs in indoor air in private homes and the risk for most of the specific cancers. Higher risk of liver cancer and meningiomas were observed. https://doi.org/10.1289/EHP10605
Operational Definitions of Treatment Response and Remission in Obsessive-Compulsive Disorder Capture Meaningful Improvements in Everyday Life
Authors: David Mataix-Cols, Erik Andersson, Kristina Aspvall, Julia Boberg, James J. Crowley, Elles de Schipper, Lorena Fernández de la Cruz, Oskar Flygare, Ekaterina Ivanova, Fabian Lenhard, Lina Lundström, Christian Rück, Eva Serlachius, Matti Cervin
Journal: Psychotherapy and Psychosomatics (2022)

&lt;b&gt;&lt;i&gt;Introduction:&lt;/i&gt;&lt;/b&gt; The operational definitions of treatment response, partial response, and remission in obsessive-compulsive disorder (OCD) are widely used in clinical trials and regular practice. However, the clinimetric sensitivity of these definitions, that is, whether they identify patients that experience meaningful changes in their everyday life, remains unexplored. &lt;b&gt;&lt;i&gt;Objective:&lt;/i&gt;&lt;/b&gt; The objective was to examine the clinimetric sensitivity of the operational definitions of treatment response, partial response, and remission in children and adults with OCD. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Pre- and post-treatment data from five clinical trials and three cohort studies of children and adults with OCD (&lt;i&gt;n&lt;/i&gt; = 1,528; 55.3% children, 61.1% female) were pooled. We compared (1) responders, partial responders, and non-responders and (2) remitters and non-remitters on self-reported OCD symptoms, clinician-rated general functioning, and self-reported quality of life. Remission was also evaluated against post-treatment diagnostic interviews. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Responders and remitters experienced large improvements across validators. Responders had greater improvements than partial responders and non-responders on self-reported OCD symptoms (Cohen’s &lt;i&gt;d&lt;/i&gt; 0.65–1.13), clinician-rated functioning (Cohen’s &lt;i&gt;d&lt;/i&gt; 0.53–1.03), and self-reported quality of life (Cohen’s &lt;i&gt;d&lt;/i&gt; 0.63–0.73). Few meaningful differences emerged between partial responders and non-responders. Remitters had better outcomes across most validators than non-remitters. Remission criteria corresponded well with absence of post-treatment diagnosis (sensitivity/specificity: 93%/83%). Using both the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) and the Clinical Global Impression Scale yielded more conservative results and more robust changes across validators, compared to only using the Y-BOCS. &lt;b&gt;&lt;i&gt;Conclusions:&lt;/i&gt;&lt;/b&gt; The current definitions of treatment response and remission capture meaningful improvements in the everyday life of individuals with OCD, whereas the concept of partial response has dubious clinimetric sensitivity.
scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells
Authors: Agostina Bianchi, Michael Scherer, Roser Zaurin, Kimberly Quililan, Lars Velten, Renée Beekman
Journal: Genome biology (2022)

Abstract Single-cell DNA methylation profiling currently suffers from excessive noise and/or limited cellular throughput. We developed scTAM-seq, a targeted bisulfite-free method for profiling up to 650 CpGs in up to 10,000 cells per experiment, with a dropout rate as low as 7%. We demonstrate that scTAM-seq can resolve DNA methylation dynamics across B-cell differentiation in blood and bone marrow, identifying intermediate differentiation states that were previously masked. scTAM-seq additionally queries surface-protein expression, thus enabling integration of single-cell DNA methylation information with cell atlas data. In summary, scTAM-seq is a high-throughput, high-confidence method for analyzing DNA methylation at single-CpG resolution across thousands of single cells.
<i>Aspergillus fumigatus</i> and personalized medicine: Toward a clinically reliable algorithm
Authors: Marco Caminati, Wojciech Feleszko, Moïse Michel, Isabella Annesi‐Maesano, Joana Vitte
Journal: Allergy (2022)

JV reports speaker and consultancy fees in the past 5 years from Meda Pharma (Mylan), Novartis, Sanofi, Thermo Fisher Scientific, outside the submitted work. The other authors declare that they have no COI relevant to this work.
Autophagic flux restoration enhances the antitumor efficacy of tumor infiltrating lymphocytes
Authors: Chaoting Zhang, Yizhe Sun, Shance Li, Luyan Shen, Xia Teng, Yefei Xiao, Nan Wu, Zheming Lu
Journal: Journal for ImmunoTherapy of Cancer (2022)

Background Although adoptive cell therapy with tumor infiltrating lymphocytes (TILs) has mediated effective antitumor responses in several cancers, dysfunction and exhaustion of TILs significantly impair the therapeutic effect of TILs. Thus, it is essential to elucidate the exhausted characteristics of TILs and improve the antitumor effect of TILs by reversing their exhaustion. Here, we focused on the influence of autophagy on TILs in terms of T-cell activation, proliferation, and differentiation in vitro and in vivo. Methods We first evaluated autophagy level of TILs and influence of spermidine treatment on autophagy levels of TILs. Furthermore, we assessed the proliferative potential, phenotypical characteristics, T cell receptor (TCR) repertoire and antitumor activity of TILs with and without spermidine treatment. Results We found that autophagic flux of TILs, especially exhausted TILs that express inhibitory immunoreceptors and have impaired proliferative capacity and decreased production of cytotoxic effector molecules, was significantly impaired. The restoration of autophagic flux via spermidine treatment resulted in increased diversity of the TCR repertoire, reduced expression of inhibitory immunoreceptors (PD1, TIM3, or LAG3), enhanced proliferation and effector functions, which subsequently demonstrated the superior in vitro and in vivo antitumor activity of TILs. Our findings unveil that spermidine, as an autophagy inducer, reverses dysfunction and exhaustion of TILs and subsequently improves the antitumor activity of TILs. Conclusions These data suggest that spermidine treatment presents an opportunity to improve adoptive TIL therapy for the treatment of solid tumors.