tbuckley/cabot-search huggingface dataset:
CHANGES IN GLOBAL LONGITUDINAL STRAIN AS A PREDICTOR OF CARDIOTOXICITY AFTER EXPOSURE TO CARFILZOMIB
Authors: Ranbir Singh, Anthony Delicce, Sophia Golec, Supreet Singh, Richa Patel, Dylan Sperling, Ashton Lai, Solomon Bienstock, Rajeev Samtani, Francesca Romana Prandi, Malcolm Anastasius, Sumeet S. Mitter, Stamatios Lerakis, Gagan D. Sahni
Journal: Journal of the American College of Cardiology (2023)
IMPROVING DISPARITIES IN VETERAN ACCESS TO TAVR: THE ROLE OF AN INTEGRATED STRUCTURAL HEART CLINIC WITHIN THE VETERANS HEALTH ADMINISTRATION
Authors: Christina C. Healy, Aneeq Waqar, Furqan Rajput, Renee Robbins, Fauzia Osman, Ruben A. Alexanian
Journal: Journal of the American College of Cardiology (2023)
IMPACT OF AORTIC VALVE CALCIUM DISTRIBUTION ON SEVERITY OF AORTIC STENOSIS
Authors: Prabhakaran Gopalakrishnan, Yan Naung Htay, Sindhuja Giridharan, Maliha Butt, Maxine Nelson, Angelena Manova, Shakthi Prabhakaran
Journal: Journal of the American College of Cardiology (2023)
NOVEL ARTIFICIAL INTELLIGENCE GUIDANCE ALGORITHM ENABLES ACQUISITION BY NOVICES OF DIAGNOSTIC QUALITY ECHOCARDIOGRAPHIC IMAGES
Authors: Robert V. Klempfner, Roberto M. Lang, Victor Mor-Avi, Brittney Guile, Kyle Hipke, Juan Ignacio Cotella, Abigail Kaminski, Merav Moreno, Dan Spiegelstein, Noa Avisar, Achiau Ludomirsky, Itay Kezurer, Bijoy K. Khandheria
Journal: Journal of the American College of Cardiology (2023)
POLYPILL-BASED STRATEGY VERSUS USUAL CARE FOR SECONDARY CARDIOVASCULAR PREVENTION: A SYSTEMATIC REVIEW AND META-ANALYSIS OF RANDOMIZED CONTROLLED TRIALS
Authors: André Rivera, Bruno de Souza Campos, Stephanie Ceolin, Carlos Alberto Campello Jorge, Isabela Reis Marques, Izabela Pera Calvi, Amanda Godoi, Everton Bruno Castanha, Rhanderson Cardoso
Journal: Journal of the American College of Cardiology (2023)
ENDOGENOUS DYSREGULATION OF THROMBOINFLAMMATORY BIOMARKERS IN END-STAGE RENAL DISEASE, AND THEIR AMPLIFICATION BY HEART FAILURE
Authors: Vanessa Robbin, Vinod Bansal, Fakiha Siddiqui, Madeline Allen, Jawed Fareed, Mushabbar A. Syed
Journal: Journal of the American College of Cardiology (2023)
REMDESIVIR ASSOCIATED BRADYCARDIA AND OUTCOMES IN COVID-19 PATIENTS: A MULTICENTER RETROSPECTIVE STUDY
Authors: Chukwuemeka Umeh, Stella Maguwudze, Harpreet Kaur, Ozivefueshe Dimowo, Niyousha Naderi, Armin Safdarpour, Rahul Gupta
Journal: Journal of the American College of Cardiology (2023)
UTILIZATION AND OUTCOMES OF IMPLANTABLE CARDIOVERTER-DEFIBRILLATOR THERAPY IN HEART FAILURE WITH CHRONIC KIDNEY DISEASE IN THE UNITED STATES FROM 2009 TO 2018
Authors: Khawaja M. Talha, Abdul Mannan Khan Minhas, Vardhmaan Jain, Muhammad Shahzeb Khan
Journal: Journal of the American College of Cardiology (2023)
Degradation behavior of ZE21C magnesium alloy suture anchors and their effect on ligament-bone junction repair
Authors: Delin Ma, Jun Wang, Mingran Zheng, Yuan Zhang, Junfei Huang, Wenxiang Li, Yiwen Ding, Yunhao Zhang, Shijie Zhu, Liguo Wang, Xiaochao Wu, Shaokang Guan
Journal: Bioactive Materials (2023)

Current materials comprising suture anchors used to reconstruct ligament-bone junctions still have limitation in biocompatibility, degradability or mechanical properties. Magnesium alloys are potential bone implant materials, and Mg2+ has been shown to promote ligament-bone healing. Here, we used Mg-2 wt.% Zn-0.5 wt.% Y-1 wt.% Nd-0.5 wt.% Zr (ZE21C) alloy and Ti6Al4V (TC4) alloy to prepare suture anchors to reconstruct the patellar ligament-tibia in SD rats. We studied the degradation behavior of the ZE21C suture anchor via in vitro and in vivo experiments and assessed its reparative effect on the ligament-bone junction. In vitro, the ZE21C suture anchor degraded gradually, and calcium and phosphorus products accumulated on its surface during degradation. In vivo, the ZE21C suture anchor could maintain its mechanical integrity within 12 weeks of implantation in rats. The tail of the ZE21C suture anchor in high stress concentration degraded rapidly during the early implantation stage (0-4weeks), while bone healing accelerated the degradation of the anchor head in the late implantation stage (4-12weeks). Radiological, histological, and biomechanical assays indicated that the ZE21C suture anchor promoted bone healing above the suture anchor and fibrocartilaginous interface regeneration in the ligament-bone junction, leading to better biomechanical strength than the TC4 group. Hence, this study provides a basis for further research on the clinical application of degradable magnesium alloy suture anchors.
VASCULAR REMODELING IN CORONARY MICROVASCULAR DYSFUNCTION
Authors: KOSHIRO SAKAI, Carlos A. Collet, Takuya Mizukami, Serena Caglioni, Frederic Bouisset, Daniel Munhoz, Marc Vanderheyden, Eric Wyffels, Jozef Bartunek, Jeroen Sonck, Emanuele Barbato, Bernard De Bruyne
Journal: Journal of the American College of Cardiology (2023)
THE REVERSIBILITY OF CARDIAC DAMAGE AFTER TRANSCATHETER AORTIC VALVE IMPLANTATION AND SHORT-TERM OUTCOMES
Authors: Rinchyenkhand Myagmardorj, Jan Stassen, Maria Chiara Meucci, Steele Butcher, Xavier Galloo, Frank Van Der Kley, Nina Ajmone Marsan, Victoria Delgado, Jeroen J. Bax
Journal: Journal of the American College of Cardiology (2023)
COMPARATIVE EFFECTIVENESS OF ENDOVASCULAR AND OPEN BYPASS INTERVENTIONS FOR BELOW THE KNEE CHRONIC LIMB-THREATENING ISCHEMIA: A PROPENSITY-MATCHED ANALYSIS USING MEDICARE-LINKED VASCULAR QUALITY INITIATIVE OUTCOMES DATA
Authors: Jacob Cleman, Gaëlle Romain, Scott Grubman, Kim G.E. Smolderen, Carlos Mena Hurtado
Journal: Journal of the American College of Cardiology (2023)
AORTIC STENOSIS BASELINE SEVERITY PREDICTS PROGRESSION: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Graeme Prosperi-Porta, Nadav Willner, Rudy R. Unni, Lawrence Lau, Pietro Di Santo, Kwan Chan, Luc Michael Beauchesne, Ian G. Burwash, David Messika-Zeitoun
Journal: Journal of the American College of Cardiology (2023)
A VERY RARE COMPLICATION OF A RELATIVELY COMMON INFECTION: ENTEROCOCCUS FAECALIS ENDOCARDITIS COMPLICATED BY VENTRICULAR SEPTAL PERFORATION
Authors: Parampreet K. Singh, Edsel Embry, Yvonne Vigil-Calderon, Muhammad W. Choudhry, Cyrus Fram Buhari, Adnan Khan
Journal: Journal of the American College of Cardiology (2023)
IMPACT OF CHRONIC HEART FAILURE ON IN-HOSPITAL OUTCOMES OF PATIENTS WITH ACUTE PULMONARY EMBOLISM
Authors: Juan Andres Quintero-Martinez, Michael Dangl, Michael Albosta, Juan Uribe, Simon Gaviria Valencia, Abel Triana, Carlos A. Vergara Sanchez, Rosario Colombo
Journal: Journal of the American College of Cardiology (2023)
CLINICAL DECISION MAKING IN A CHALLENGING CASE: A CASE OF ANOMALOUS ORIGIN OF THE RIGHT CORONARY ARTERY WITH INTERARTERIAL COURSE PRESENTS WITH SUDDEN CARDIAC DEATH
Authors: Israa O. Al-gburi, Amid Bitar, Reem Al Abed Alqader, Firas El Sabbagh
Journal: Journal of the American College of Cardiology (2023)
LIPOPROTEIN (A) LEVEL INCREASES PLAQUE VULNERABILITY AND PANCORONARY LIPID VOLUME IN PATIENTS WITH CORONARY HEART DISEASE: A 3-VESSEL OPTICAL COHERENCE TOMOGRAPHY STUDY
Authors: Bin Wang, Xiao Wang, Wen Hao, Ge Wang, Yuekun Zhang, Shaoping Nie
Journal: Journal of the American College of Cardiology (2023)
POTENTIAL CLINICAL UTILITY OF OXYRVAD FOR THE MANAGEMENT OF COMBINED RIGHT VENTRICULAR AND RESPIRATORY FAILURE: A SYSTEMATIC REVIEW.
Authors: Juliette Beaulieu, Hamza Ouazani Chahdi, Christine Vu, Sanjog Kalra, Alexis Matteau, Samer Mansour, Marc E. Jolicoeur, Pasquale Ferraro, Brian J. Potter
Journal: Journal of the American College of Cardiology (2023)
NOT YOUR COMMON CAUSE OF PALPITATIONS: CARDIAC PARAGANGLIOMA IN YOUNG ADULT
Authors: Erica G. Otero-Cardenas, William Redondo, Andres Cordova Toro, Hector Nunez Medina, Maria Alejandra Rodriguez-Santiago, Gisela D. Puig-Carrion, Hector Luis Banchs Pieretti
Journal: Journal of the American College of Cardiology (2023)
A LOTUS UPSTREAM; UNUSUAL CASE OF RETROGRADE TAVR VALVE MIGRATION
Authors: Andrew Corrigan, Mehak Kapoor, Ralph Cipriano, Kevin Phillip Marzo, Richard K. Schwartz, Khaled Salhab, Todd C. Kerwin, Daniel Donohue, Gregory Macina, Beevash Ray
Journal: Journal of the American College of Cardiology (2023)
SUBCLAVIAN, VERTEBRAL AND INTERNAL MAMMARY ARTERIAL THROMBOSIS WITH PULMONARY EMBOLISM COMPLICATING COVID-19 INFECTION
Authors: Hany Youssef, Matthew Meleka, Zain A. Abidi, Omar Obaid, Ali Alkhayru, Merrill A. Krolick
Journal: Journal of the American College of Cardiology (2023)
A CASE OF THE 'VARSITY BLUES’: SVC OBSTRUCTION IN A RECREATIONAL ATHLETE
Authors: Sanjay Sivalokanathan, Christopher Kusmiesz, Alejandro de Feria, Sara L. Partington, Stephanie Fuller, Rajat Deo, Sharlene M. Day, Helene Glassberg, Sheela Krishnan, Neel Chokshi
Journal: Journal of the American College of Cardiology (2023)
INDEPENDENT AND INCREMENTAL PROGNOSTIC VALUE OF D-DIMER IN ACUTE CORONARY SYNDROME PATIENTS UNDERGOING PCI: DATA FROM A LARGE PROSPECTIVE COHORT STUDY
Authors: Sida Jia, Yuan Deshan, Ying Song, Jingjing Xu, Xueyan Zhao, Jinqing Yuan
Journal: Journal of the American College of Cardiology (2023)
STREPTOCOCCUS AGALACTIAE ENDOCARDITIS PRESENTING AS PULMONARY EMBOLISM IN A PATIENT WITH ADULT CONGENITAL HEART DISEASE
Authors: Nakeya Dewaswala, Khadija Amanullah, Huzefa Bhopalwala, Amrita Iyengar, Attila Ahmad, Vincent L. Sorrell
Journal: Journal of the American College of Cardiology (2023)
PROTEIN S DEFFICIENCY ASOCIATED TO ACUTE PULMONARY AND MYOCARDIAL INFARCTION, IN A 26 YEAR OLD WOMEN WITH HISTORY OF HELLP SYNDROME AND TRANSIENT ISCHEMIC ATTACK
Authors: Alexis Morales Guzman, Pamela Ramirez Rangel, José Alberto Sánchez Toscano, Laura Marcela Ospina Restrepo, Alexandra Arias-Mendoza
Journal: Journal of the American College of Cardiology (2023)
SERIAL 3D PRINTING TO PLAN A STAGED PALLIATION IN A PATIENT WITH COMPLEX CONGENITAL HEART DISEASE AND MAJOR AORTOPULMONARY COLLATERALS
Authors: Fahad Alfares, Michael Bunker, Phillip Moore, Mark W. Cocalis, Shravan Sridhar, Kimberly Kallianos, Naveen Swami, V. Mohan Reddy, Shafkat Anwar
Journal: Journal of the American College of Cardiology (2023)
CORONARY EVENTS IN VALVE-IN-VALVE TAVR AND NATIVE TAVR - A TWO-YEAR MATCHED COHORT
Authors: Ofir Koren, Vivek Patel, Alon Shechter, Robert Naami, Tarun Chakravarty, Mamoo Nakamura, Wen Cheng, Zev Aaron Allison, Hasan Jilaihawi, Raj R. Makkar
Journal: Journal of the American College of Cardiology (2023)
EFFECT OF A CLINICAL DECISION SUPPORT TOOL FOR FAMILIAL HYPERCHOLESTEROLEMIA ON LOW-DENSITY LIPOPROTEIN-CHOLESTEROL LEVELS: A PRE-POST IMPLEMENTATION STUDY
Authors: Hana Bangash, Seyedmohammad Saadatagah, Lubna Alhalabi, Marwan Hamed, Alborz Sherafati, Joseph Sutton, Omar Elsekaily, Ali Mir, Justin Gundelach, Dan Gibbons, Paul Johnsen, Carin Smith, Christina Wood-Wentz, Daniel Crusan, Kent R. Bailey, Pedro Caraballo, Iftikhar J. Kullo
Journal: Journal of the American College of Cardiology (2023)
EVALUATING ACCURACY OF ESTIMATED VO2MAX WITH WRIST WORN POLAR IGNITE COMPARED TO PEAK VO2 ON FORMAL CARDIOPULMONARY EXERCISE TESTING IN HEALTHY AND FONTAN PEDIATRIC PATIENTS
Authors: BRIAN HERNANDEZ, Bayley Roberts, Anusha Kodidhi, Lisa Roelle, Nathan Miller, Lauren M. Littell, Jonathan R. Silva, George F. Van Hare, Jennifer A. Silva, William Orr
Journal: Journal of the American College of Cardiology (2023)
ATRIAL FIBRILLATION ABLATIONS IN THE UNITED STATES-A UNIQUE CURRENT PROCEDURAL TERMINOLOGY (CPT) CODE-BASED ANALYSIS 2016-2019.
Authors: Dinesh Voruganti, Shivaraj Patil, Tyler Alderson, Bader Alotaibi, Murrium Sadaf, Subodh Devabhaktuni, Paul Mounsey, Hakan Paydak
Journal: Journal of the American College of Cardiology (2023)
HOSPITAL VISITS OF PATIENTS ON CARDIAC IMPLANTABLE ELECTRONIC DEVICES (CIEDS) REMOTE MONITORING DURING THE COVID-19 PANDEMIC: SOCIOECONOMIC IMPLICATIONS
Authors: Jiyoung Seo, Amrin Kharawala, Suraj Krishnan, Majd Al Deen Alhuarrat, Dimitrios Varrias, Samuel J. Apple, Ibrahim Selevany, Jay Chudow, Andrew Krumerman, Kevin J. Ferrick
Journal: Journal of the American College of Cardiology (2023)
OUTCOMES OF ACUTE CORONARY SYNDROME IN PATIENTS WITH INTELLECTUAL DISABILITY: INSIGHTS FROM THE NATIONAL INPATIENT SAMPLE
Authors: Ahmed M. Maraey, Hadeer Rafat Elsharnoby, Mobeen Z. Haider, Yousra Khalid, Mahmoud Salem, Mahmoud Khalil, Ahmed Younes, Shazia Aziz, Ahmed Elzanaty
Journal: Journal of the American College of Cardiology (2023)
CARDIAC COMPLICATIONS AMONG LONG COVID PATIENTS: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Joanna Lee, Aditi S. Kothari, Gaurang Bhatt, Navneet Gupta, Ahmed Elsaid Ali, Nadia Najam, Muhammad Mazroua, Taha Mansoor, Tanya Amal, Mariam Elsaban, Rohan Deo, Vikas Bansal, Rahul Kashyap
Journal: Journal of the American College of Cardiology (2023)
Immune checkpoint blockade induces gut microbiota translocation that augments extraintestinal antitumor immunity
Authors: Yongbin Choi, Jake N. Lichterman, Laura A. Coughlin, Nicole Poulides, Wenling Li, Priscilla Del Valle, Suzette N. Palmer, Shuheng Gan, Jiwoong Kim, Xiaowei Zhan, Yajing Gao, Bret M. Evers, Lora V. Hooper, Chandrashekhar Pasare, Andrew Y. Koh
Journal: Science Immunology (2023)

Gut microbiota, specifically gut bacteria, are critical for effective immune checkpoint blockade therapy (ICT) for cancer. The mechanisms by which gut microbiota augment extraintestinal anticancer immune responses, however, are largely unknown. Here, we find that ICT induces the translocation of specific endogenous gut bacteria into secondary lymphoid organs and subcutaneous melanoma tumors. Mechanistically, ICT induces lymph node remodeling and dendritic cell (DC) activation, which facilitates the translocation of a selective subset of gut bacteria to extraintestinal tissues to promote optimal antitumor T cell responses in both the tumor-draining lymph nodes (TDLNs) and the primary tumor. Antibiotic treatment results in decreased gut microbiota translocation into mesenteric lymph nodes (MLNs) and TDLNs, diminished DC and effector CD8
ATRIAL FIBRILLATION PRIOR TO HEART FAILURE ONSET IS ASSOCIATED WITH ADVERSE OUTCOMES IN PATIENTS REGARDLESS OF LEFT VENTRICULAR EJECTION FRACTION
Authors: Pradeep Rajendran, Yuxi Liu, Sharon Grossman, Melvin Joice, James A. Alzate, Davis Kimaiyo, Christy Taylor, Reza Mohebi, Kemar Brown, Kyra Weaver, Jason Chen, Mia Grayson, James L. Januzzi, Hanna Kim Gaggin
Journal: Journal of the American College of Cardiology (2023)
GEOGRAPHICAL VARIATION IN PATIENT CHARACTERISTICS AND OUTCOMES IN HFPEF
Authors: John J.V. McMurray, Mingming Yang, Toru Kondo, Pardeep Jhund, Marco Antonio Alcocer Gamba, Jan Belohlavek, Jan Willem Borleffs, Chern-En Chiang, Josep Comin Colet, Akshay S. Desai, Dan Dobreanu, Jaroslaw Drozdz, Yaling Han, Stefan P. Janssens, Tzvetana M. Katova, Mikhail Kosiborod, Carolyn S.P. Lam, Anna Maria Langkilde, Bela Merkely, Vinh Pham, jorge thierer, Muthiah Vaduganathan, Subodh Verma, Scott D. Solomon
Journal: Journal of the American College of Cardiology (2023)
Fc glycoengineering of a PD-L1 antibody harnesses Fcγ receptors for increased antitumor efficacy
Authors: Noy Cohen Saban, Adam Yalin, Tomer Landsberger, Ran Salomon, Ajjai Alva, Tali Feferman, Ido Amit, Rony Dahan
Journal: Science Immunology (2023)

FDA-approved anti-PD-L1 monoclonal antibodies (mAbs) bear the IgG1 isotype, whose scaffolds are either wild-type (e.g., avelumab) or Fc-mutated and lacking Fcγ receptor (FcγR) engagement (e.g., atezolizumab). It is unknown whether variation in the ability of the IgG1 Fc region to engage FcγRs renders mAbs with superior therapeutic activity. In this study, we used humanized FcγR mice to study the contribution of FcγR signaling to the antitumor activity of human anti-PD-L1 mAbs and to identify an optimal human IgG scaffold for PD-L1 mAbs. We observed similar antitumor efficacy and comparable tumor immune responses in mice treated with anti-PD-L1 mAbs with wild-type and Fc-mutated IgG scaffolds. However, in vivo antitumor activity of the wild-type anti-PD-L1 mAb avelumab was enhanced by combination treatment with an FcγRIIB-blocking antibody, which was co-administered to overcome the suppressor function of FcγRIIB in the tumor microenvironment (TME). We performed Fc glycoengineering to remove the fucose subunit from the Fc-attached glycan of avelumab to enhance its binding to the activating FcγRIIIA. Treatment with the Fc-afucosylated version of avelumab also enhanced antitumor activity and induced stronger antitumor immune responses compared with the parental IgG. The enhanced effect by afucosylated PD-L1 antibody was dependent on neutrophils and associated with decreased frequencies of PD-L1+ myeloid cells and increased infiltration of T cells in the TME. Our data reveal that the current design of FDA-approved anti-PD-L1 mAbs does not optimally harness FcγR pathways and suggest two strategies to enhance FcγR engagement to optimize anti-PD-L1 immunotherapy.
CD19 CAR antigen engagement mechanisms and affinity tuning
Authors: Changhao He, Jorge Mansilla-Soto, Nandish Khanra, Mohamad Hamieh, Victor Bustos, Alice J. Paquette, Andreina Garcia Angus, Derek M. Shore, William J. Rice, George Khelashvili, Michel Sadelain, Joel R. Meyerson
Journal: Science Immunology (2023)

Chimeric antigen receptor (CAR) T cell therapy relies on T cells that are guided by synthetic receptors to target and lyse cancer cells. CARs bind to cell surface antigens through an scFv (binder), the affinity of which is central to determining CAR T cell function and therapeutic success. CAR T cells targeting CD19 were the first to achieve marked clinical responses in patients with relapsed/refractory B cell malignancies and to be approved by the U.S. Food and Drug Administration (FDA). We report cryo-EM structures of CD19 antigen with the binder FMC63, which is used in four FDA-approved CAR T cell therapies (Kymriah, Yescarta, Tecartus, and Breyanzi), and the binder SJ25C1, which has also been used extensively in multiple clinical trials. We used these structures for molecular dynamics simulations, which guided creation of lower- or higher-affinity binders, and ultimately produced CAR T cells endowed with distinct tumor recognition sensitivities. The CAR T cells exhibited different antigen density requirements to trigger cytolysis and differed in their propensity to prompt trogocytosis upon contacting tumor cells. Our work shows how structural information can be applied to tune CAR T cell performance to specific target antigen densities.
PRIMARY ANGIOPLASTY IN A PATIENT WITH CONGENITALLY CORRECTED TRANSPOSITION OF THE GREAT ARTERIES
Authors: Enma Toledo, Jardiel Arguello, Alejandro Alcaraz Guzman, Gerardo Chacon Loyola, Jimena Gonzalez Salido, NORMAN SAID VEGA, Edgar Garcia, Diego Araiza Garaygordobil
Journal: Journal of the American College of Cardiology (2023)
HISTORICAL NEIGHBORHOOD REDLINING CONTINUES TO ADVERSELY INFLUENCE CARDIOVASCULAR OUTCOMES IN US VETERANS WITH ATHEROSCLEROTIC CARDIOVASCULAR DISEASE
Authors: Sadeer G. Al-Kindi, Issam Motairek, Salim S. Virani, Khurram Nasir, Sanjay Rajagopalan, Salil Deo
Journal: Journal of the American College of Cardiology (2023)
PRESENCE OF TRANSTHYRETIN CARDIAC AMYLOIDOSIS AS AN INDEPENDENT RISK FACTOR OF MORBIDITY REGARDLESS OF EJECTION FRACTION
Authors: Mileydis Alonso, Yelenis Seijo De Armas, Malcolm Lawson McDonald, Antonio Lewis, Alejandro Sanchez, Dipan Uppal, Abdullah Sarkar, Manojna Nimmagadda, Sachin Kumar, Mauricio Velez, David A. Baran, Jerry D. Estep, David G. Wolinsky, David Snipelisky
Journal: Journal of the American College of Cardiology (2023)
Pure Conducting Polymer Hydrogels Increase Signal‐to‐Noise of Cutaneous Electrodes by Lowering Skin Interface Impedance
Authors: Sebastian Roubert Martinez, Paul Le Floch, Jia Liu, Robert D. Howe
Journal: Advanced Healthcare Materials (2023)

Cutaneous electrodes are routinely used for noninvasive electrophysiological sensing of signals from the brain, the heart, and the neuromuscular system. These bioelectronic signals propagate as ionic charge from their sources to the skin-electrode interface where they are then sensed as electronic charge by the instrumentation. However, these signals suffer from low signal-to-noise ratio arising from the high impedance at the tissue-to-electrode contact interface. This paper reports that soft conductive polymer hydrogels made purely of poly(3,4-ethylenedioxy-thiophene) doped with poly(styrene sulfonate) present nearly an order of magnitude decrease in the skin-electrode contact impedance (88%, 82%, and 77% at 10, 100, and 1 kHz, respectively) when compared to clinical electrodes in an ex vivo model that isolates the bioelectrochemical features of a single skin-electrode contact. Integrating these pure soft conductive polymer blocks into an adhesive wearable sensor enables high fidelity bioelectronic signals with higher signal-to-noise ratio (average 2.1 dB increase, max 3.4 dB increase) when compared to clinical electrodes across all subjects. The utility of these electrodes is demonstrated in a neural interface application. The conductive polymer hydrogels enable electromyogram-based velocity control of a robotic arm to complete a pick and place task. This work provides a basis for the characterization and use of conductive polymer hydrogels to better couple human and machine.
Single immunizations of self-amplifying or non-replicating mRNA-LNP vaccines control HPV-associated tumors in mice
Authors: Jamile Ramos da Silva, Karine Bitencourt Rodrigues, Guilherme Formoso Pelegrin, Natiely Silva Sales, Hiromi Muramatsu, Mariângela de Oliveira Silva, Bruna F. M. M. Porchia, Ana Carolina Ramos Moreno, Luana Raposo M. M. Aps, Aléxia Adrianne Venceslau-Carvalho, István Tombácz, Wesley Luzetti Fotoran, Katalin Karikó, Paulo J. C. Lin, Ying K. Tam, Mariana de Oliveira Diniz, Norbert Pardi, Luís Carlos de Souza Ferreira
Journal: Science Translational Medicine (2023)

As mRNA vaccines have proved to be very successful in battling the coronavirus disease 2019 (COVID-19) pandemic, this new modality has attracted widespread interest for the development of potent vaccines against other infectious diseases and cancer. Cervical cancer caused by persistent human papillomavirus (HPV) infection is a major cause of cancer-related deaths in women, and the development of safe and effective therapeutic strategies is urgently needed. In the present study, we compared the performance of three different mRNA vaccine modalities to target tumors associated with HPV-16 infection in mice. We generated lipid nanoparticle (LNP)-encapsulated self-amplifying mRNA as well as unmodified and nucleoside-modified non-replicating mRNA vaccines encoding a chimeric protein derived from the fusion of the HPV-16 E7 oncoprotein and the herpes simplex virus type 1 glycoprotein D (gDE7). We demonstrated that single low-dose immunizations with any of the three gDE7 mRNA vaccines induced activation of E7-specific CD8+ T cells, generated memory T cell responses capable of preventing tumor relapses, and eradicated subcutaneous tumors at different growth stages. In addition, the gDE7 mRNA-LNP vaccines induced potent tumor protection in two different orthotopic mouse tumor models after administration of a single vaccine dose. Last, comparative studies demonstrated that all three gDE7 mRNA-LNP vaccines proved to be superior to gDE7 DNA and gDE7 recombinant protein vaccines. Collectively, we demonstrated the immunogenicity and therapeutic efficacy of three different mRNA vaccines in extensive comparative experiments. Our data support further evaluation of these mRNA vaccines in clinical trials.