AB0523 THE ENTEROTYPES OF THE GUT MICROBIOTA IN CHINESE POPULATION WITH AUTOIMMUNE DISEASE
Authors: M. J. Chang, S. X. Zhang, J. Qiao, C. Wang, H. R. Chen, T. Huang, Q. Yu, P. F. He
Journal: Annals of the Rheumatic Diseases (2022)

Background An increasing number of autoimmune disorders (AD) have been associated with microbial dysbiosis[1, 2]. However, this dysbiosis is difficult to characterize for individual patients owing to the high heterogeneity of the gut microbiota. Thus, researchers must find an accurate method of characterizing the AD gut microbiota that is meaningful to clinical diagnosis. Objectives The aim of this study was to investigate the enterotype characters of intestinal flora in AD and their associations with peripheral lymphocyte subpopulations and cytokines. Methods A total of 339 AD patients and 339 age- and sex- matched healthy controls (HCs) were enrolled in this study. Mathematical modeling using Dirichlet multinomial mixtures (DMM) was applied to describe the variability in the microbiome data and cluster samples into enterotypes. The peripheral lymphocyte subsets were detected by flow cytometry and the cytokines were assessed by ELISA. Differential abundance analysis was carried out the STAMP software. R (version 4.1.0) was used for comparative statistics, and spearman’s correlation analysis was used to assess the correlations between the relative abundances of bacterial genera and clinical variables. Results Laplace approximation of DMM suggested gut microbiota of AD patients and HCs both can be divided into two distinct enterotypes (Figure 1 A-B), and AD E1 and HC E1 were primarily dominated by Prevotella while AD E2 and HC E2 by Bacteroides. Interestingly, the Prevotella-enriched enterotype (AD E1 and HC E1) had a higher alpha diversity than The Bacteroides-enriched enterotype (AD E2 and HC E2). Patients with AD always had a lower richness and diversity compared with those of HCs in each enterotype (p&lt; 0.001), suggesting gut microbiome was markedly less diverse in composition in AD. Bray curtis distance-based beta-diversity were also different (P&lt;0.001, ANOSIM.R =0.23, Figure 1 C-H). Significant differences in gut microbiota composition at the genus level between AD patients and HCs were found using the STAMP software in each enterotype. Compared with HCs, 37 species in AD E1 patients and 40 species in AD E2 patients of flora were discovered to be distinctly different. In the co-upregulated flora of both enterotypes, Lactobacillus was inversely associated with a variety of lymphocytes such as T, CD4+T, NK, Th2, Th17, Treg cells(P&lt;0.05), and positive correlation with IL-10 and IFN-γ(P&lt;0.05,Figure 1 I). However, in the co-downregulated floras Coprococcus had a positive correlation with B, NK and Treg cells, and anaerostipes had a negativate corrleation with IL-2 and IL-4(P&lt;0.05,Figure 1 J). Conclusion There were both two enterotypes in patients and HCs with autoimmune disease, E2 exhibited a loss of Prevotella but a growth of Bacteroides, while E1 presented the opposite results, which were closely correlated with peripheral lymphocyte subsets and cytokines. References [1]Levy M, Thaiss CA, Zeevi D, Dohnalová L, Zilberman-Schapira G, Mahdi JA, David E, Savidor A, Korem T, Herzig Y et al: Microbiota-Modulated Metabolites Shape the Intestinal Microenvironment by Regulating NLRP6 Inflammasome Signaling. Cell 2015, 163(6):1428-1443. [2]Belkaid Y, Hand TW: Role of the microbiota in immunity and inflammation. Cell 2014, 157(1):121-141. Acknowledgements This work was supported by the National Natural Science Foundation of China (No. 82001740). Disclosure of Interests None declared