tbuckley/cabot-search huggingface dataset:
Two‐Photon Light Trigger siRNA Transfection of Cancer Cells Using Non‐Toxic Porous Silicon Nanoparticles
Authors: Arnaud Chaix, Eduardo Cueto‐Diaz, Sofia Dominguez‐Gil, Chantelle Spiteri, Laure Lichon, Marie Maynadier, Xavier Dumail, Dina Aggad, Anthony Delalande, Aurélie Bessière, Chantal Pichon, Ciro Chiappini, Michael J. Sailor, Nadir Bettache, Magali Gary‐Bobo, Jean‐Olivier Durand, Christophe Nguyen, Frédérique Cunin
Journal: Advanced Healthcare Materials (2023)

The concept of using two-photon excitation in the NIR for the spatiotemporal control of biological processes holds great promise. However, its use for the delivery of nucleic acids has been very scarcely described and the reported procedures are not optimal as they often involve potentially toxic materials and irradiation conditions. This work prepares a simple system made of biocompatible porous silicon nanoparticles (pSiNP) for the safe siRNA photocontrolled delivery and gene silencing in cells upon two-photon excitation. PSiNP are linked to an azobenzene moiety, which possesses a lysine group (pSiNP@ICPES-azo@Lys) to efficiently complex siRNA. Non-linear excitation of the two-photon absorber system (pSiNP) followed by intermolecular energy transfer (FRET) to trans azobenzene moiety, result in the photoisomerization of the azobenzene from trans to cis and in the destabilization of the azobenzene-siRNA complex, thus inducing the delivery of the cargo siRNA to the cytoplasm of cells. Efficient silencing in MCF-7 expressing stable firefly luciferase with siRNAluc against luciferase is observed. Furthermore, siRNA against inhibitory apoptotic protein (IAP) leads to over 70% of MCF-7 cancer cell death. The developed technique using two-photon light allows a unique high spatiotemporally controlled and safe siRNA delivery in cells in few seconds of irradiation.
Reply to Liu et al.
Authors: Jad Farha, Saowanee Ngamruengphong
Journal: Endoscopy (2023)

See also: Helix tack-and-suture device for mucosal defect closure following colorectal endoscopic submucosal dissection: is it ready for global adoption?Endoscopy 2023; 55(08): 780-780DOI: 10.1055/a-2046-5159
Treatment-related mortality in children with cancer in low-income and middle-income countries: a systematic review and meta-analysis
Authors: Bella S Ehrlich, Michael J McNeil, Linh T D Pham, Yichen Chen, Jocelyn Rivera, Carlos Acuna, Liz Sniderman, Firas M Sakaan, Alejandra Mendez Aceituno, Cesar A Villegas, Lisa M Force, Nancy S Bolous, Parima P Wiphatphumiprates, Jeremy S Slone, Angela K Carrillo, Srinithya R Gillipelli, Caitlyn Duffy, Anita V Arias, Meenakshi Devidas, Carlos Rodriguez-Galindo, Sheena Mukkada, Asya Agulnik
Journal: The Lancet Oncology (2023)
Endoscopy International Open – recently published
Journal: Endoscopy (2023)

Comment on: Efficacy and safety of peroral endoscopic myotomy for esophageal diverticulaEndosc Int Open 2023; 11(05): E546-E552DOI: 10.1055/a-2071-6744 Comment on: Endoscopic ultrasound-directed transgastric ERCP (EDGE): A multicenter US study on long-term follow-up and fistula closureEndosc Int Open 2023; 11(05): E529-E537DOI: 10.1055/a-2057-5984 Comment on: The Colonoscopy Cookbook: Analysis of effect of a recipe resource on quality and experience of bowel preparation in colonoscopyEndosc Int Open 2023; 11(05): E561-E565DOI: 10.1055/a-2057-4374
Endoscopy E-Videos – recently published
Journal: Endoscopy (2023)

Comment on: Contrast enhancement for undetectable intrahepatic bile duct to facilitate endoscopic ultrasound-guided hepaticogastrostomyEndoscopy 2023; 55(S 01): E511-E512DOI: 10.1055/a-2032-3512 Comment on: Novel low echo reduction function reduces artifacts in endoscopic ultrasound observation of the gallbladderEndoscopy 2023; 55(S 01): E529-E531DOI: 10.1055/a-2037-5802 Comment on: Snare-tip soft coagulation to treat esophageal stent epithelial hyperplasiaEndoscopy 2023; 55(S 01): E572-E573DOI: 10.1055/a-2050-7431 Comment on: Novel resection technique for large flat colorectal tumors: Clip-line-assisted underwater endoscopic mucosal resectionEndoscopy 2023; 55(S 01): E585-E586DOI: 10.1055/a-2051-8154
Author Correction: Large-scale encoding of emotion concepts becomes increasingly similar between individuals from childhood to adolescence
Authors: M. Catalina Camacho, Ashley N. Nielsen, Dori Balser, Emily Furtado, David C. Steinberger, Leah Fruchtman, Joseph P. Culver, Chad M. Sylvester, Deanna M. Barch
Journal: Nature Neuroscience (2023)
An MMP‐2 Responsive Nanotheranostic Probe Enabled Synergistic Therapy of Rheumatoid Arthritis and MR/CT Assessment of Therapeutic Response In Situ
Authors: Xuan Chen, Deshen Dai, Junping Ma, Zipei Yu, Junkai Zhao, Changqing Yi
Journal: Advanced Healthcare Materials (2023)

This study reports a facile and green synthesis of a new multifunctional nanotheranostic probe for the synergistic therapy of rheumatoid arthritis (RA) and in situ assessment of therapeutic response. The probe is synthesized through a one-step self-assembly of two exquisitely designed peptide-amphiphilic block copolymers (PEG-DTIPA-KGPLGVRK-MTX and Pal-GGGGHHHHD-TCZ) under mild conditions, requiring minimal energy input. The resultant probe demonstrates excellent biocompatibility, water solubility, and colloidal stability. It exhibits a strong IL-6R targeting ability toward inflamed joints, and releases drugs in an MMP-2-responsive manner. The co-loading of methotrexate(MTX) and tocilizumab (TCZ) into the probe enables synergistic RA therapy with improved efficacy by simultaneously decreasing the activity of adenosine synthetase and interfering with the binding of IL-6 to its receptor. In addition, the resultant probe exhibits a high r1 relaxation rate (7.00 mm-1 s-1 ) and X-ray absorption capability (69.04 Hu mm-1 ), enabling sensitive MR and CT dual-modal imaging for simultaneous evaluation of synovial thickness and bone erosion. Both in vitro experiments using lipopolysaccharide-treated RAW264.7 cells and in vivo experiments using collagen-induced arthritis mice demonstrate the probe's high effectiveness in synergistically inhibiting inflammation. This study provides new insights into RA theranostics, therapeutic monitoring, the design of multifunctional theranostic probes, and beyond.
Cognitive Function in Patients with Chronic Respiratory Failure: Effect of Treatment with Home Noninvasive Ventilation
Authors: Maxime Patout, Léa Razakamanantsoa, Rebecca D’Cruz, Gill Arbane, Thomas Similowski, Nicholas Hart, Patrick B. Murphy
Journal: American Journal of Respiratory and Critical Care Medicine (2023)

"Cognitive Function in Patients with Chronic Respiratory Failure: Effect of Treatment with Home Noninvasive Ventilation." American Journal of Respiratory and Critical Care Medicine, 208(8), pp. 900–903
Commentary
Authors: Stefan Seewald, Tiing Leong Ang
Journal: Endoscopy (2023)

Through-the-scope (TTS) clips are ideal for managing iatrogenic perforation during endoscopic resection as these clips can be applied immediately without the need for any additional preparation. When there is a large defect or when there is surrounding fibrosis, it can be challenging to capture and appose the two edges of the perforation securely using TTS clips. TTS clips with nylon purse-string suturing, over-the-scope (OTS) clips, and endoscopic suturing devices have been used to overcome such challenges. However, these other techniques require additional preparation time and are more cumbersome to use than TTS clips.
Clinical characteristics and outcomes of patients with cirrhosis and hepatocellular carcinoma in The Gambia, west Africa: a prospective cohort study
Authors: Gibril Ndow, Erwan Vo-Quang, Yusuke Shimakawa, Amie Ceesay, Saydiba Tamba, Harr F Njai, Lamin Bojang, Charlotte Hateley, Yuki Takao, Emmanuel Opoku, Zakary Warsop, Patrick Ingiliz, Umberto D'Alessandro, Isabelle Chemin, Maimuna Mendy, Mark Thursz, Ramou Njie, Maud Lemoine
Journal: The Lancet Global Health (2023)
Momelotinib versus danazol in symptomatic patients with anaemia and myelofibrosis previously treated with a JAK inhibitor (MOMENTUM): an updated analysis of an international, double-blind, randomised phase 3 study
Authors: Aaron T Gerds, Srdan Verstovsek, Alessandro M Vannucchi, Haifa Kathrin Al-Ali, David Lavie, Andrew T Kuykendall, Sebastian Grosicki, Alessandra Iurlo, Yeow Tee Goh, Mihaela C Lazaroiu, Miklos Egyed, Maria Laura Fox, Donal McLornan, Andrew Perkins, Sung-Soo Yoon, Vikas Gupta, Jean-Jacques Kiladjian, Nikki Granacher, Sung-Eun Lee, Luminita Ocroteala, Francesco Passamonti, Claire N Harrison, Stephen Oh, Barbara J Klencke, Jing Yu, Rafe Donahue, Jun Kawashima, Ruben Mesa
Journal: The Lancet Haematology (2023)
Immunogenicity, safety, and preliminary efficacy evaluation of OVX836, a nucleoprotein-based universal influenza A vaccine candidate: a randomised, double-blind, placebo-controlled, phase 2a trial
Authors: Isabel Leroux-Roels, Paul Willems, Gwenn Waerlop, Yorick Janssens, Jessika Tourneur, Fien De Boever, Jacques Bruhwyler, Azhar Alhatemi, Bart Jacobs, Florence Nicolas, Geert Leroux-Roels, Alexandre Le Vert
Journal: The Lancet Infectious Diseases (2023)
Overcome the “Buckets Effect”: Integration of AIEgens into Proteins for Fluorescence‐Enhanced Two‐Photon Imaging
Authors: Miaozhuang Fan, Zhengzheng Li, Gang Feng, Yibin Zhang, Wenguang Zhang, Chengbin Yang, Yonghong Shao, Changrui Liao, Gaixia Xu, Zhourui Xu
Journal: Advanced Healthcare Materials (2023)

Luminogens with aggregation-induced emission characteristics (AIEgens) are considered good options for two-photon (2P) probes, owing to their flexibility of design, heavy-metal-free composition, and resistance to photobleaching. However, the design principles for large 2P absorption cross-section (δ) generally require high coplanarity, strong donor-acceptor (D-A) interactions, and long conjugation, which can severely weaken the brightness of AIEgens at the aggregated state and undermine their potential in 2P fluorescence imaging (2PFI). Exploration of a feasible approach to overcome the "Buckets Effect" of AIEgen-based 2P probes is thus a fascinating yet challenging task. Herein, an AIEgen, namely (Z)-2-(4-aminophenyl)-3-(5-(4-(bis(4-methoxyphenyl)amino)phenyl)thiophen-2-yl)acrylonitrile (MTAA) is designed to have a big δ according to the calculation result and a low fluorescence quantum yield (QY) of 2.2% in dimethyl sulfoxide (DMSO). Impressively, upon integrating into bovine serum albumin (BSA), the protein-sized MTAA@BSA dots exhibit a 25-fold higher fluorescence QY compared to MTAA molecules, contributing to an imaging depth of 818 µm in the brain vasculature. The retention and clearance of MTAA@BSA dots in the liver and kidney are also studied using 2PFI. Overall, this work provides a facile approach to overcome the "Buckets Effect" of AIEgen to generate highly efficient, reliable, and biocompatible 2P probes.
Pulse oximetry values from 33,080 participants in the Apple Heart &amp; Movement Study
Authors: Ian Shapiro, Jeff Stein, Calum MacRae, Michael O’Reilly
Journal: npj Digital Medicine (2023)

Wearable devices that include pulse oximetry (SpO2) sensing afford the opportunity to capture oxygen saturation measurements from large cohorts under naturalistic conditions. We report here a cross-sectional analysis of 72 million SpO2 values collected from 33,080 individual participants in the Apple Heart and Movement Study, stratified by age, sex, body mass index (BMI), home altitude, and other demographic variables. Measurements aggregated by hour of day into 24-h SpO2 profiles exhibit similar circadian patterns for all demographic groups, being approximately sinusoidal with nadir near midnight local time, zenith near noon local time, and mean 0.8% lower saturation during overnight hours. Using SpO2 measurements averaged for each subject into mean nocturnal and daytime SpO2 values, we employ multivariate ordinary least squares regression to quantify population-level trends according to demographic factors. For the full cohort, regression coefficients obtained from models fit to daytime SpO2 are in close quantitative agreement with the corresponding values from published reference models for awake arterial oxygen saturation measured under controlled laboratory conditions. Regression models stratified by sex reveal significantly different age- and BMI-dependent SpO2 trends for females compared with males, although constant terms and regression coefficients for altitude do not differ between sexes. Incorporating categorical variables encoding self-reported race/ethnicity into the full-cohort regression models identifies small but statistically significant differences in daytime SpO2 (largest coefficient corresponding to 0.13% lower SpO2, for Hispanic study participants compared to White participants), but no significant differences between groups for nocturnal SpO2. Additional stratified analysis comparing regression models fit independently to subjects in each race/ethnicity group is suggestive of small differences in age- and sex-dependent trends, but indicates no significant difference in constant terms between any race/ethnicity groups for either daytime or nocturnal SpO2. The large diverse study population and study design employing automated background SpO2 measurements spanning the full 24-h circadian cycle enables the establishment of healthy population reference trends outside of clinical settings.
Relationship Between Symptom Perception and Postprandial Glycemic Profiles in Patients With Postbariatric Hypoglycemia After Roux-en-Y Gastric Bypass Surgery
Authors: Afroditi Tripyla, Antonio Ferreira, Katja A. Schönenberger, Noah H. Näf, Lukas E. Inderbitzin, Francesco Prendin, Luca Cossu, Giacomo Cappon, Andrea Facchinetti, David Herzig, Lia Bally
Journal: Diabetes Care (2023)

Post-bariatric surgery hypoglycemia (PBH) is a metabolic complication of Roux-en-Y gastric bypass (RYGB). Since symptoms are a key component of the Whipple's triad to diagnose nondiabetic hypoglycemia, we evaluated the relationship between self-reported symptoms and postprandial sensor glucose profiles.Thirty patients with PBH after RYGB (age: 50.1 [41.6-60.6] years, 86.7% female, BMI: 26.5 [23.5-31.2] kg/m2; median [interquartile range]) wore a blinded Dexcom G6 sensor while recording autonomic, neuroglycopenic, and gastrointestinal symptoms over 50 days. Symptoms (overall and each type) were categorized into those occurring in postprandial periods (PPPs) without hypoglycemia, or in the preceding dynamic or hypoglycemic phase of PPPs with hypoglycemia (nadir sensor glucose <3.9 mmol/L). We further explored the relationship between symptoms and the maximum negative rate of sensor glucose change and nadir sensor glucose levels.In 5,851 PPPs, 775 symptoms were reported, of which 30.6 (0.0-59.9)% were perceived in PPPs without hypoglycemia, 16.7 (0.0-30.1)% in the preceding dynamic phase and 45.0 (13.7-84.7)% in the hypoglycemic phase of PPPs with hypoglycemia. Per symptom type, 53.6 (23.8-100.0)% of the autonomic, 30.0 (5.6-80.0)% of the neuroglycopenic, and 10.4 (0.0-50.0)% of the gastrointestinal symptoms occurred in the hypoglycemic phase of PPPs with hypoglycemia. Both faster glucose dynamics and lower nadir sensor glucose levels were related with symptom perception.The relationship between symptom perception and PBH is complex, challenging clinical judgement and decision-making in this population.
Carbazole and tetrahydro-carboline derivatives as dopamine D3 receptor antagonists with the multiple antipsychotic-like properties
Authors: Zhongtang Li, Fan Fang, Yiyan Li, Xuehui Lv, Ruqiu Zheng, Peili Jiao, Yuxi Wang, Guiwang Zhu, Zefang Jin, Xiangqing Xu, Yinli Qiu, Guisen Zhang, Zhongjun Li, Zhenming Liu, Liangren Zhang
Journal: Acta Pharmaceutica Sinica B (2023)

Dopamine D3 receptor (D3R) is implicated in multiple psychotic symptoms. Increasing the D3R selectivity over dopamine D2 receptor (D2R) would facilitate the antipsychotic treatments. Herein, novel carbazole and tetrahydro-carboline derivatives were reported as D3R selective ligands. Through a structure-based virtual screen, ZLG-25 (D3R Ki= 685 nmol/L; D2R Ki>10,000 nmol/L) was identified as a novel D3R selective bitopic ligand with a carbazole scaffold. Scaffolds hopping led to the discovery of novel D3R-selective analogs with tetrahydro-β-carboline or tetrahydro-γ-carboline core. Further functional studies showed that most derivatives acted as hD3R-selective antagonists. Several lead compounds could dose-dependently inhibit the MK-801-induced hyperactivity. Additional investigation revealed that 23j and 36b could decrease the apomorphine-induced climbing without cataleptic reaction. Furthermore, 36b demonstrated unusual antidepressant-like activity in the forced swimming tests and the tail suspension tests, and alleviated the MK-801-induced disruption of novel object recognition in mice. Additionally, preliminary studies confirmed the favorable PK/PD profiles, no weight gain and limited serum prolactin levels in mice. These results revealed that 36b provided potential opportunities to new antipsychotic drugs with the multiple antipsychotic-like properties.
Improving outcomes of in situ split liver transplantation in Italy over the last 25 years
Authors: Andrea Lauterio, Umberto Cillo, Marco Spada, Silvia Trapani, Riccardo De Carlis, Giuliano Bottino, Davide Bernasconi, Chiara Scalamogna, Domenico Pinelli, Davide Cintorino, Francesco Enrico D’Amico, Gionata Spagnoletti, Marco Miggino, Renato Romagnoli, Leonardo Centonze, Lucio Caccamo, Umberto Baccarani, Amedeo Carraro, Matteo Cescon, Marco Vivarelli, Vincenzo Mazaferro, Giuseppe Maria Ettorre, Massimo Rossi, Giovanni Vennarecci, Paolo De Simone, Roberta Angelico, Salvatore Agnes, Fabrizio Di Benedetto, Luigi Giovanni Lupo, Fausto Zamboni, Arianna Zefelippo, Damiano Patrono, Pietro Diviacco, Zoe Larghi Laureiro, Enrico Gringeri, Fabrizio Di Francesco, Alessandro Lucianetti, Maria Grazia Valsecchi, Salvatore Gruttadauria, Tullia De Feo, Massimo Cardillo, Luciano De Carlis, Michele Colledan, Enzo Andorno
Journal: Journal of Hepatology (2023)
Advances in the Development of Granular Microporous Injectable Hydrogels with Non‐spherical Microgels and Their Applications in Tissue Regeneration
Authors: Haiyan Li, Keerthi Subramanian Iyer, Lei Bao, Jiali Zhai, Jiao Jiao Li
Journal: Advanced Healthcare Materials (2023)

Granular microporous hydrogels are emerging as effective biomaterial scaffolds for tissue engineering due to their improved characteristics compared to traditional nanoporous hydrogels, which better promote cell viability, cell migration, cellular/tissue infiltration, and tissue regeneration. Recent advances have resulted in the development of granular hydrogels made of non-spherical microgels, which compared to those made of spherical microgels have higher macroporosity, more stable mechanical properties, and better ability to guide the alignment and differentiation of cells in anisotropic tissue. The development of these hydrogels as an emerging research area is attracting increasing interest in regenerative medicine. This review first summarizes the fabrication techniques available for non-spherical microgels with different aspect-ratios. Then, it introduces the development of granular microporous hydrogels made of non-spherical microgels, their physicochemical characteristics, and their applications in tissue regeneration. The limitations and future outlook of research on microporous granular hydrogels are also critically discussed.
Three-Dimensional Collision Avoidance Method for Robot-Assisted Minimally Invasive Surgery
Authors: Ling Li, Xiaojian Li, Bo Ouyang, Hangjie Mo, Hongliang Ren, Shanlin Yang
Journal: Cyborg and Bionic Systems (2023)

In the robot-assisted minimally invasive surgery, if a collision occurs, the robot system program could be damaged, and normal tissues could be injured. To avoid collisions during surgery, a 3-dimensional collision avoidance method is proposed in this paper. The proposed method is predicated on the design of 3 strategic vectors: the collision-with-instrument-avoidance (CI) vector, the collision-with-tissues-avoidance (CT) vector, and the constrained-control (CC) vector. The CI vector demarcates 3 specific directions to forestall collision among the surgical instruments. The CT vector, on the other hand, comprises 2 components tailored to prevent inadvertent contact between the robot-controlled instrument and nontarget tissues. Meanwhile, the CC vector is introduced to guide the endpoint of the robot-controlled instrument toward the desired position, ensuring precision in its movements, in alignment with the surgical goals. Simulation results verify the proposed collision avoidance method for robot-assisted minimally invasive surgery. The code and data are available at https://github.com/cynerelee/collision-avoidance.
Hierarchically Porous Implants Orchestrating a Physiological Viscoelastic and Piezoelectric Microenvironment for Bone Regeneration
Authors: Zheng‐Min Zhang, Peng Yu, Kai Zhou, Fan‐Yuan Yu, Rui‐Ying Bao, Ming‐Bo Yang, Zhi‐Yong Qian, Wei Yang
Journal: Advanced Healthcare Materials (2023)

The extracellular matrix microenvironment of bone tissue comprises several physiological cues. Thus, artificial bone substitute materials with a single cue are insufficient to meet the demands for bone defect repair. Regeneration of critical-size bone defects remains challenging in orthopedic surgery. Intrinsic viscoelastic and piezoelectric cues from collagen fibers play crucial roles in accelerating bone regeneration, but scaffolds or implants providing integrated cues have seldom been reported. In this study, it is aimed to design and prepare hierarchically porous poly(methylmethacrylate)/polyethyleneimine/poly(vinylidenefluoride) composite implants presenting a similar viscoelastic and piezoelectric microenvironment to bone tissue via anti-solvent vapor-induced phase separation. The viscoelastic and piezoelectric cues of the composite implants for human bone marrow mesenchymal stem cell line stimulate and activate Piezo1 proteins associated with mechanotransduction signaling pathways. Cortical and spongy bone exhibit excellent regeneration and integration in models of critical-size bone defects on the knee joint and femur in vivo. This study demonstrates that implants with integrated physiological cues are promising artificial bone substitute materials for regenerating critical-size bone defects.
Tumor aerobic glycolysis confers immune evasion through modulating sensitivity to T cell-mediated bystander killing via TNF-α
Authors: Lijian Wu, Yiteng Jin, Xi Zhao, Kaiyang Tang, Yaoning Zhao, Linjie Tong, Xuerong Yu, Ke Xiong, Ce Luo, Jiajun Zhu, Fubing Wang, Zexian Zeng, Deng Pan
Journal: Cell Metabolism (2023)
Single-molecule genome-wide mutation profiles of cell-free DNA for non-invasive detection of cancer
Authors: Daniel C. Bruhm, Dimitrios Mathios, Zachariah H. Foda, Akshaya V. Annapragada, Jamie E. Medina, Vilmos Adleff, Elaine Jiayuee Chiao, Leonardo Ferreira, Stephen Cristiano, James R. White, Sarah A. Mazzilli, Ehab Billatos, Avrum Spira, Ali H. Zaidi, Jeffrey Mueller, Amy K. Kim, Valsamo Anagnostou, Jillian Phallen, Robert B. Scharpf, Victor E. Velculescu
Journal: Nature Genetics (2023)

Somatic mutations are a hallmark of tumorigenesis and may be useful for non-invasive diagnosis of cancer. We analyzed whole-genome sequencing data from 2,511 individuals in the Pan-Cancer Analysis of Whole Genomes (PCAWG) study as well as 489 individuals from four prospective cohorts and found distinct regional mutation type-specific frequencies in tissue and cell-free DNA from patients with cancer that were associated with replication timing and other chromatin features. A machine-learning model using genome-wide mutational profiles combined with other features and followed by CT imaging detected >90% of patients with lung cancer, including those with stage I and II disease. The fixed model was validated in an independent cohort, detected patients with cancer earlier than standard approaches and could be used to monitor response to therapy. This approach lays the groundwork for non-invasive cancer detection using genome-wide mutation features that may facilitate cancer screening and monitoring.