tbuckley/cabot-search huggingface dataset:
Correction: Gamabufotalin, a bufadienolide compound from toad venom, suppresses COX-2 expression through targeting IKKβ/NF-κB signaling pathway in lung cancer cells
Authors: Zhenlong Yu, Wei Guo, Xiaochi Ma, Baojing Zhang, Peipei Dong, Lin Huang, Xiuli Wang, Chao Wang, Xiaokui Huo, Wendan Yu, Canhui Yi, Yao Xiao, Wenjing Yang, Yu Qin, Yuhui Yuan, Songshu Meng, Quentin Liu, Wuguo Deng
Journal: Molecular Cancer (2023)

Unfortunately, the original version of this article [1] contained some errors.The words "binding" has been spelt incorrectly in the fifth title of result section and legend of Fig. 5.And the image of GAPDH (Fig. 6D) has been mistakenly uploaded.The mistakes did not affect any correctness of our results or discussion.The corrected sentence Fig. 6 was provided here.We regret any inconvenience that caused."CS-6 inhibited NF-κB and p300 translocation and binding to COX-2 promoter"."Figure 5 CS-6 inhibited NF-κB and p300 translocation and their binding to COX-2 promoter".
A noninvasive method for whole-genome skin methylome profiling
Authors: Cristiana Banila, Daniel Green, Dimitris Katsanos, Joana Viana, Alice Osmaston, Angel Menendez Vazquez, Magnus Lynch, Shakiba Kaveh
Journal: British Journal of Dermatology (2023)

Abstract Background Ageing, disease and malignant transformation of the skin are associated with changes in DNA methylation. So far, mostly invasive methodologies such as biopsies have been applied in collecting DNA methylation signatures. Tape stripping offers a noninvasive option for skin diagnostics. It enables the easy but robust capture of biologic material in large numbers of participants without the need for specialized medical personnel. Objectives To design and validate a methodology for noninvasive skin sample collection using tape stripping for subsequent DNA ­methylation analysis. Methods A total of 175 participants were recruited and provided tape-stripping samples from a sun-exposed area; 92 provided matched tape-stripping samples from a sun-protected area, and an additional 5 provided matched skin-shave biopsies from the same area. Using ­enzymatic conversion and whole-genome Illumina sequencing, we generated genome-wide DNA methylation profiles that were used to evaluate the feasibility of noninvasive data acquisition, to compare with established sampling approaches and to investigate biomarker identification for age and ultraviolet (UV) exposure. Results We found that tape-stripping samples showed strong concordance in their global DNA methylation landscapes to those of conventional invasive biopsies. Moreover, we showed sample reproducibility and consistent global methylation profiles in skin tape-stripping samples collected from different areas of the body. Using matched samples from sun-protected and sun-exposed areas of the body we were able to validate the capacity of our method to capture the effects of environmental changes and ageing in a cohort covering various ages, ethnicities and skin types. We found DNA methylation changes on the skin resulting from UV exposure and identified significant age-related hypermethylation of CpG islands, with a pronounced peak effect at 50–55 years of age, including methylation changes in well-described markers of ageing. Conclusions These data demonstrate the feasibility of using tape stripping combined with whole-genome sequencing as a noninvasive approach to measuring DNA methylation changes in the skin. In addition, they outline a viable experimental framework for the use of skin tape stripping, particularly when it is performed in large cohorts of patients to identify biomarkers of skin ageing, UV damage and, possibly, to track treatment response to therapeutic interventions.
Identification of NIFTP-Specific mRNA Markers for Reliable Molecular Diagnosis of Thyroid Tumors
Authors: So-Yeon Lee, Jong-Lyul Park, Kwangsoon Kim, Ja Seong Bae, Jae-Yoon Kim, Seon-Young Kim, Chan Kwon Jung
Journal: Endocrine Pathology (2023)

Non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) is a low-risk thyroid tumor with a favorable prognosis. Nonetheless, differentiating NIFTP from other thyroid tumors remains challenging, necessitating reliable diagnostic markers. This study is aimed at discovering NIFTP-specific mRNA markers through RNA sequencing analysis of thyroid tumor tissues. We performed mRNA expression profiling for 74 fresh frozen thyroid tissue samples, including NIFTP and benign and malignant follicular-cell-derived tumors. NIFTP/malignant tumors showed 255 downregulated genes and 737 upregulated genes compared to benign tumors. Venn diagram analysis revealed 19 significantly upregulated and 7 downregulated mRNAs in NIFTP. Akaike information criterion analysis allowed us to select OCLN, ZNF423, LYG1, and AQP5 mRNA markers. We subsequently developed a predictive model based on logistic regression analysis using these four mRNAs, which we validated in independent samples (n = 90) using a qRT-PCR assay. This model demonstrated high accuracy in predicting NIFTP in discovery dataset (AUC (area under the receiver operating characteristic) = 0.960) and the validation dataset (AUC = 0.757). Our results suggest that OCLN, ZNF423, LYG1, and AQP5 mRNA markers might serve as reliable molecular markers for identifying NIFTP among other thyroid tumors, ultimately aiding in accurate diagnosis and management of NIFTP patients.
Functional outcomes and quality of life at 1-year follow-up after an open tibia fracture in Malawi: a multicentre, prospective cohort study
Authors: Alexander Thomas Schade, Maureen Sabawo, Nohakhelha Nyamulani, Chikumbutso Clara Mpanga, Leonard Banza Ngoie, Andrew John Metcalfe, David G Lalloo, Jason J Madan, William James Harrison, Peter MacPherson
Journal: The Lancet Global Health (2023)

BackgroundInjuries are a major cause of disability globally and injury incidence is rapidly increasing, largely due to road traffic injuries in low-income and middle-income countries. Current estimates of the scale and consequences of disability from injury are largely based on modelling studies, with a scarcity of empirical evidence from severe injuries in low-income countries. We aimed to better understand the outcomes for individuals with open tibia fractures in Malawi.MethodsIn this multicentre, prospective cohort study, adults (aged ≥18 years) with open tibia fractures were systematically recruited at six hospitals in Malawi (two tertiary hospitals and four district hospitals). Follow-up lasted at least 1 year, during which in-person follow-up reviews were done at 6 weeks, 3 months, 6 months, and 1 year post-injury. The primary outcome was function at 1 year post-injury, measured by the Short Musculoskeletal Functional Assessment (SMFA) score. Secondary outcomes included quality-adjusted life-years (QALYs; as determined via the European Quality of Life 5-Dimensions 3-Levels [EQ-5D-3L] survey) and fracture-related infection at 1 year post-injury. Multilevel regression models investigated associations between SMFA score, EQ-5D-3L, baseline factors, and orthopaedic management.FindingsBetween Feb 12, 2021, and March 14, 2022, 287 participants were enrolled (median age 34 years [IQR 25–44]; 84% male). The most common mode of injury was road traffic injuries (194 [68%] of 287). Overall, 268 (93%) participants had debridement; of the 63 participants who were debrided in district hospitals, 47 (75%) had the procedure under local or no anaesthesia. Following substantial declines by 6 weeks after injury, function and quality of life had not recovered by 1 year post-injury for participants with Gustilo grade I–II fractures (posterior mean SMFA at 1 year: 10·5, 95% highest density interval [HDI]: 9·5–11·6; QALYs: 0·73, 95% HDI: 0·66–0·80) nor Gustilo grade III fractures (posterior mean SMFA at 1 year: 14·9, 95% HDI: 13·4–16·6; QALYs: 0·67, 95% HDI: 0·59–0·75). For all fracture grades, intramedullary nailing substantially improved function and quality of life at 1 year post-injury. Delayed definitive fixation after 5 days had 5-times greater odds of infection compared with early management within 2 days (adjusted odds ratio: 5·1, 95% CI 1·8–16·1; p=0·02).InterpretationAdults with open tibia fractures in Malawi have poor function and quality of life in the 1 year following injury. Centralised orthopaedic surgical management, including early definitive fixation and intramedullary nailing for more severe injuries, might improve outcomes.FundingWellcome Trust.TranslationFor the Chichewa translation of the abstract see Supplementary Materials section.
Corrigendum to “Pembrolizumab Plus Axitinib Versus Sunitinib as First-line Treatment of Advanced Renal Cell Carcinoma: 43-month Follow-up of the Phase 3 KEYNOTE-426 Study” [Eur. Urol. (2023)]
Authors: Elizabeth R. Plimack, Thomas Powles, Viktor Stus, Rustem Gafanov, Dmitry Nosov, Tom Waddell, Boris Alekseev, Frédéric Pouliot, Bohuslav Melichar, Denis Soulières, Delphine Borchiellini, Raymond S. McDermott, Ihor Vynnychenko, Yen-Hwa Chang, Satoshi Tamada, Michael B. Atkins, Chenxiang Li, Rodolfo Perini, L. Rhoda Molife, Jens Bedke, Brian I. Rini
Journal: European Urology (2023)
Combination of biodegradable hydrogel and antioxidant bioadhesive for treatment of breast cancer recurrence and radiation skin injury
Authors: Zhuodan Zhang, Qiannan Cao, Yi Xia, Chunyan Cui, Ying Qi, Qian Zhang, Yuanhao Wu, Jianfeng Liu, Wenguang Liu
Journal: Bioactive Materials (2023)

Postoperative radiotherapy is the standard method for inhibition of breast cancer recurrence and metastasis, whereas radiation resistant and ineluctable skin radiation injury are still key problems encountered in the prognosis of breast cancer. Herein, we design an internally implantable biodegradable hydrogel and extracutaneously applicable antioxidant bioadhesive to concurrently prevent postoperative tumor recurrence and radioactive skin injury after adjuvant radiotherapy. The biodegradable silk fibroin/perfluorocarbon hydrogel loading doxorubicin (DOX) formed by consecutive ultrasonication-induced β-sheets-crosslinked amphiphilic silk fibroin/perfluorocarbon/DOX nanoemulsion, exhibits continuous release of oxygen in physiological environment to improve hypoxia and sensitivity of radiotherapy, as well as simultaneous release of DOX to finally achieve effective anti-cancer effect. A stretchable bioadhesive is fabricated by copolymerization of α-thioctic acid and N, N-diacryloyl-l-lysine, and gold nanorods and gallic acid are loaded into the bioadhesive to afford gentle photothermal therapy and antioxidant functions. The near-infrared light-induced controlled release of gallic acid and mild photothermal therapy can efficiently eliminate excess free radicals generated by radiotherapy and promote radioactive wound healing. Ultimately, in vivo animal studies substantiate the efficacy of our methodology, wherein the post-tumor resection administration of hydrogel and concomitant application of an antioxidant bioadhesive patch effectively inhibit tumor recurrence and attenuate the progression of skin radiation damage.
Secondary Mutations of the EGFR Gene That Confer Resistance to Mobocertinib in EGFR Exon 20 Insertion
Authors: Akira Hamada, Kenichi Suda, Masaya Nishino, Keiko Obata, Hana Oiki, Tomoyo Fukami, Shota Fukuda, Toshio Fujino, Shuta Ohara, Takamasa Koga, Masato Chiba, Masaki Shimoji, Masaoki Ito, Toshiki Takemoto, Junichi Soh, Yasuhiro Tsutani, Tetsuya Mitsudomi
Journal: Journal of Thoracic Oncology (2023)
A detailed evaluation of the effect of dupilumab on sleep in adults with atopic dermatitis
Authors: Erika Y Lee, Aaron M Drucker
Journal: British Journal of Dermatology (2023)

Journal Article Corrected proof A detailed evaluation of the effect of dupilumab on sleep in adults with atopic dermatitis Get access Erika Y Lee, Erika Y Lee Conceptualization, Writing - original draft, Writing - review & editing Division of Clinical Immunology and Allergy, Department of Medicine, St. Michael’s Hospital, Toronto, ON, Canada; Divisions ofClinical Immunology and AllergyInstitute of Health Policy, Management and Evaluation, University of Toronto, Toronto, ON, Canada Correspondence: Erika Y. Lee. Email: erika.lee@mail.utoronto.ca https://orcid.org/0000-0003-1108-2067 Search for other works by this author on: Oxford Academic Google Scholar Aaron M Drucker Aaron M Drucker Conceptualization, Supervision, Writing - review & editing Dermatology, Department of MedicineInstitute of Health Policy, Management and Evaluation, University of Toronto, Toronto, ON, CanadaDivision of Dermatology, Department of MedicineWomen’s College Research Institute, Women’s College Hospital, Toronto, ON, Canada Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, ljad320, https://doi.org/10.1093/bjd/ljad320 Published: 02 September 2023 Article history Received: 25 August 2023 Accepted: 27 August 2023 Published: 02 September 2023 Corrected and typeset: 30 September 2023
Nemolizumab to target the itch–scratch cycle in paediatric atopic dermatitis
Authors: Antonia Lloyd-Lavery
Journal: British Journal of Dermatology (2023)

Journal Article Corrected proof Nemolizumab to target the itch–scratch cycle in paediatric atopic dermatitis Get access Antonia Lloyd-Lavery Antonia Lloyd-Lavery Formal analysis Department of Dermatology, Oxford University Hospitals NHS Foundation Trust, Oxford, UK Email: antonia.lloyd-lavery@ouh.nhs.uk https://orcid.org/0000-0002-2339-0596 Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, ljad319, https://doi.org/10.1093/bjd/ljad319 Published: 02 September 2023 Article history Received: 25 August 2023 Accepted: 27 August 2023 Published: 02 September 2023 Corrected and typeset: 11 October 2023
The role of enhancers in psoriasis and atopic dermatitis
Authors: Jennifer Y Sui, Dawn Z Eichenfield, Bryan K Sun
Journal: British Journal of Dermatology (2023)

Abstract Regulatory elements, particularly enhancers, play a crucial role in disease susceptibility and progression. Enhancers are DNA sequences that activate gene expression and can be affected by epigenetic modifications, interactions with transcription factors (TFs) or changes to the enhancer DNA sequence itself. Altered enhancer activity impacts gene expression and contributes to disease. In this review, we define enhancers and the experimental techniques used to identify and characterize them. We also discuss recent studies that examine how enhancers contribute to atopic dermatitis (AD) and psoriasis. Articles in the PubMed database were identified (from 1 January 2010 to 28 February 2023) that were relevant to enhancer variants, enhancer-associated TFs and enhancer histone modifications in psoriasis or AD. Most enhancers associated with these conditions regulate genes affecting epidermal homeostasis or immune function. These discoveries present potential therapeutic targets to complement existing treatment options for AD and psoriasis.
Advances in immunotherapy for triple-negative breast cancer
Authors: Yang Liu, Yueting Hu, Jinqi Xue, Jingying Li, Jiang Yi, Jiawen Bu, Zhenyong Zhang, Peng Qiu, Xi Gu
Journal: Molecular Cancer (2023)

Immunotherapy has recently emerged as a treatment strategy which stimulates the human immune system to kill tumor cells. Tumor immunotherapy is based on immune editing, which enhances the antigenicity of tumor cells and increases the tumoricidal effect of immune cells. It also suppresses immunosuppressive molecules, activates or restores immune system function, enhances anti-tumor immune responses, and inhibits the growth f tumor cell. This offers the possibility of reducing mortality in triple-negative breast cancer (TNBC).Immunotherapy approaches for TNBC have been diversified in recent years, with breakthroughs in the treatment of this entity. Research on immune checkpoint inhibitors (ICIs) has made it possible to identify different molecular subtypes and formulate individualized immunotherapy schedules. This review highlights the unique tumor microenvironment of TNBC and integrates and analyzes the advances in ICI therapy. It also discusses strategies for the combination of ICIs with chemotherapy, radiation therapy, targeted therapy, and emerging treatment methods such as nanotechnology, ribonucleic acid vaccines, and gene therapy. Currently, numerous ongoing or completed clinical trials are exploring the utilization of immunotherapy in conjunction with existing treatment modalities for TNBC. The objective of these investigations is to assess the effectiveness of various combined immunotherapy approaches and determine the most effective treatment regimens for patients with TNBC.This review provides insights into the approaches used to overcome drug resistance in immunotherapy, and explores the directions of immunotherapy development in the treatment of TNBC.
Threatening the Future of Global Health — NIH Policy Changes on International Research Collaborations
Authors: Albert I. Ko, Salim S. Abdool Karim, Carlos Morel, Soumya Swaminathan, Peter Daszak, Gerald T. Keusch
Journal: New England Journal of Medicine (2023)

Interview with Salim Abdool Karim on a National Institutes of Health policy that will add new requirements for foreign subrecipients of funding. (08:19)Download A new policy for foreign subrecipients of NIH funding departs sharply from decades of efforts to promote research integrity and build research capacity globally — and may have wide-ranging negative consequences.
CircPTEN suppresses human clear cell renal carcinoma progression and resistance to mTOR inhibitors by targeting epigenetic modification
Authors: Yangyang Zhan, Yang Liu, Rui Yang, Qiong Chen, Fei Teng, Yueying Huang, Xin Jiang, Yueming Wang, Bin Yu, Ding Zhang, Leilei Bao, Xinli Liu, Jiwei Huang
Journal: Drug Resistance Updates (2023)
<i>Lonomia obliqua</i> Envenomation
Authors: Nicolas Peruzzo, Gustavo Gössling
Journal: New England Journal of Medicine (2023)

A 44-year-old man presented with a 4-day history of spontaneous bruising and bloody urine. Three hours before symptom onset, he was walking in the woods in Brazil and felt a sharp prick on the back of his thigh.
Logic gate controlled theranostic nanoagents for in situ microwave thermal therapeutic efficacy evaluation
Authors: Zengzhen Chen, Wenna Guo, Tiansong Liang, Yingjuan Zheng, Meng Niu, Daoke Yang, Longfei Tan, Changhui Fu, Qiong Wu, Xiangling Ren, Jie Yu, Ping Liang, Jun Ren, Xianwei Meng
Journal: Biomaterials (2023)
A Dual‐Antioxidative Coating on Transmucosal Component of Implant to Repair Connective Tissue Barrier for Treatment of Peri‐Implantitis
Authors: Dize Li, Xi Tan, Liwen Zheng, Han Tang, Shanshan Hu, Qiming Zhai, Xuan Jing, Panpan Liang, Yuxin Zhang, Qingqing He, Guangyu Jian, Dongqi Fan, Ping Ji, Tao Chen, Hongmei Zhang
Journal: Advanced Healthcare Materials (2023)

Since the microgap between implant and surrounding connective tissue creates the pass for pathogen invasion, sustained pathological stimuli can accelerate macrophage-mediated inflammation, therefore affecting peri-implant tissue regeneration and aggravate peri-implantitis. As the transmucosal component of implant, the abutment therefore needs to be biofunctionalized to repair the gingival barrier. Here, a mussel-bioinspired implant abutment coating containing tannic acid (TA), cerium and minocycline (TA-Ce-Mino) is reported. TA provides pyrogallol and catechol groups to promote cell adherence. Besides, Ce3+ /Ce4+ conversion exhibits enzyme-mimetic activity to remove reactive oxygen species while generating O2 , therefore promoting anti-inflammatory M2 macrophage polarization to help create a regenerative environment. Minocycline is involved on the TA surface to create local drug storage for responsive antibiosis. Moreover, the underlying therapeutic mechanism is revealed whereby the coating exhibits exogenous antioxidation from the inherent properties of Ce and TA and endogenous antioxidation through mitochondrial homeostasis maintenance and antioxidases promotion. In addition, it stimulates integrin to activate PI3K/Akt and RhoA/ROCK pathways to enhance VEGF-mediated angiogenesis and tissue regeneration. Combining the antibiosis and multidimensional orchestration, TA-Ce-Mino repairs soft tissue barriers and effector cell differentiation, thereby isolating the immune microenvironment from pathogen invasion. Consequently, this study provides critical insight into the design and biological mechanism of abutment surface modification to prevent peri-implantitis.
Dual‐Targeted Nanoparticle‐in‐Microparticle System for Ulcerative Colitis Therapy
Authors: Yawen Zhang, Yue Wu, Yuping Yan, Yijing Ma, Linglan Tu, Jingjing Shao, Xuanyu Tang, Lingfeng Chen, Guang Liang, Lina Yin
Journal: Advanced Healthcare Materials (2023)

Abstract Conventional oral therapy for ulcerative colitis (UC) is associated with premature release or degradation of drugs in the harsh gastrointestinal environment, resulting in reduced therapeutic effectiveness. Consequently, the present study aims to develop a dual‐targeted delivery system with a nanoparticle‐in‐microparticle (nano‐in‐micro) structure. The prepared Asiatic Acid‐loaded delivery system (AA/CDM‐BT‐ALG) has pH‐sensitive properties. Cellular uptake evaluation confirms that nanoparticles exhibit targeted absorption by macrophages and Caco‐2 cells through mannose (Man) receptor and biotin‐mediated endocytosis, respectively. Therefore, this mechanism effectively enhances intracellular drug concentration. Additionally, the biodistribution study conducted on the gastrointestinal tract of mice indicates that the colon of the microspheres group shows higher fluorescence intensity with longer duration than the other groups. This finding indicates that the microspheres exhibit selective accumulation in areas of colon inflammation. In vivo experiments in colitis mice showed that AA/CDM‐BT‐ALG significantly alleviates the histopathological characteristics of the colon, reduced neutrophil, and macrophage infiltration, and decreases pro‐inflammatory cytokine expression. Furthermore, the effect of AA/CDM‐BT‐ALG on colitis is validated to be closely related to the TLR4/MyD88/NF‐κB signaling pathway. The present findings suggest that the development of a dual‐targeted delivery system is accomplished effectively, with the potential to serve as a drug‐controlled release system for treating UC.
Antioxidant‐Engineered Milk‐Derived Extracellular Vesicles for Accelerating Wound Healing via Regulation of the PI3K‐AKT Signaling Pathway
Authors: Limin Fan, Xiaoyi Ma, Bingbing Liu, Yushan Yang, Yan Yang, Tianbin Ren, Yongyong Li
Journal: Advanced Healthcare Materials (2023)

Abstract Inspired by the experience of relieving inflammation in infants with milk, antioxidant‐engineered milk‐derived extracellular vesicles (MEVs) are developed to evaluate their potential for accelerating wound healing. In this work, MEVs with polydopamines (PDA) are engineered using the co‐extrusion method. Subsequently, the authors incorporated them into a Schiff‐based crosslink hydrogel, forming a skin dosage form that could facilitate the wound healing process. The antioxidant properties of PDA assist in the anti‐inflammatory function of engineered MEVs, while the gel provides better skin residency. The PDA@MEVs+GEL formulation exhibits excellent biocompatibility, pro‐angiogenic capacity, and antioxidant ability in vitro. Furthermore, in vivo experiments demonstrate its efficacy in wound repair and inflammation inhibition. Mechanistically, PDA@MEVs+GEL simultaneously promotes the growth, migration, and anti‐inflammation of 3T3 cells by activating PI3K‐AKT pathway. Moreover, PDA@MEVs+GEL exhibits enhanced functionality in promoting wound healing in vivo, attributed to its ability to inhibit inflammation, stimulate angiogenesis, and promote collagen synthesis. In conclusion, this study delves into the mechanism of MEVs and underscores the improved efficacy of engineered extracellular vesicles. Additionally, the feasibility and prospect of engineered MEVs in treating skin wounds are verified, suggesting that antioxidant‐engineered MEVs could be a promising therapeutic agent for wound healing applications.