tbuckley/cabot-search huggingface dataset:
The Effect of Cryoprotectants and Storage Conditions on the Transfection Efficiency, Stability, and Safety of Lipid‐Based Nanoparticles for mRNA and DNA Delivery
Authors: Konstantinos N. Kafetzis, Natalia Papalamprou, Elisha McNulty, Kai X. Thong, Yusuke Sato, Aleksandr Mironov, Atul Purohit, Philip J. Welsby, Hideyoshi Harashima, Cynthia Yu‐Wai‐Man, Aristides D. Tagalakis
Journal: Advanced Healthcare Materials (2023)

Abstract Lipid‐based nanoparticles have recently shown great promise, establishing themselves as the gold standard in delivering novel RNA therapeutics. However, research on the effects of storage on their efficacy, safety, and stability is still lacking. Herein, the impact of storage temperature on two types of lipid‐based nanocarriers, lipid nanoparticles (LNPs) and receptor‐targeted nanoparticles (RTNs), loaded with either DNA or messenger RNA (mRNA), is explored and the effects of different cryoprotectants on the stability and efficacy of the formulations are investigated. The medium‐term stability of the nanoparticles was evaluated by monitoring their physicochemical characteristics, entrapment and transfection efficiency, every two weeks over one month. It is demonstrated, that the use of cryoprotectants protects nanoparticles against loss of function and degradation in all storage conditions. Moreover, it is shown that the addition of sucrose enables all nanoparticles to remain stable and maintain their efficacy for up to a month when stored at −80 °C, regardless of cargo or type of nanoparticle. DNA‐loaded nanoparticles also remain stable in a wider variety of storage conditions than mRNA‐loaded ones. Importantly, these novel LNPs show increased GFP expression that can signify their future use in gene therapies, beyond the established role of LNPs in RNA therapeutics.
Evidence on the existence of sublineages of the current human monkeypox virus: time for in depth study
Authors: Chiranjib Chakraborty, Manojit Bhattacharya, Kuldeep Dhama, Govindasamy Agoramoorthy
Journal: International Journal of Surgery (2023)

Chakraborty, Chiranjib PhD; Bhattacharya, Manojit PhD; Dhama, Kuldeep PhD; Agoramoorthy, Govindasamy PhD Author Information
Sudan ebolavirus (SUDV) outbreak in Uganda: transmission, risk assessment, prevention, and mitigation strategies – correspondence
Authors: Shailesh K. Patel, Nikhil KC, Jigyasa Rana, Aditya Agrawal, Dhruv N. Desai, Pawan D.S. Raghuvanshi, Shailendra Singh, Bhabesh C. Das, Tejinder Kaur
Journal: International Journal of Surgery (2023)

Patel, Shailesh K. PhD; KC, Nikhil PhD; Rana, Jigyasa PhD; Agrawal, Aditya PhD; Desai, Dhruv N. PhD; Raghuvanshi, Pawan D.S. PhD; Singh, Shailendra PhD; Das, Bhabesh C. PhD; Kaur, Tejinder PhD Author Information
Recent concern about the outbreak of Crimean-Congo hemorrhagic fever: virology, etiology, pathogenesis, symptoms, transmission, diagnostics, and treatment
Authors: Md. Rezaul Islam, Puja S. Dhar, Md. Mominur Rahman
Journal: International Journal of Surgery (2023)

Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Dhaka, Bangladesh This manuscript has been peer reviewed. Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article. Published online ■ ■ *Corresponding author. Address: Faculty of Allied Health Sciences, Daffodil International University, Dhaka 1207, Bangladesh. Tel: +8801730-627585; E-mail address: [email protected] (Md. M. Rhaman). This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/
Efficacy and safety of the surgical treatments for lower calyceal stones: a systematic review and network meta-analysis
Authors: Jun Hou, Feihong Xu, Huifang Du, Jingxuan Liu, Ning Li
Journal: International Journal of Surgery (2023)

Objective: Efficacy and safety of five common surgical treatments for lower calyceal (LC) stones were assessed for LC stones 20 mm or less. Methods: A systematic literature search was conducted up to June 2020 using PubMed, EMBASE, and Cochrane Library. The study has been registered in PROSPERO, CRD42021228404. Randomized controlled trials evaluating the efficacy and safety of five common surgical treatments for LC stones were collected, including percutaneous nephrolithotomy (PCNL), mini-PCNL (MPCNL), ultramini-PCNL (UMPCNL), extracorporeal shock wave lithotripsy (ESWL), and retrograde intrarenal surgery (RIRS). Heterogeneity among studies was assessed by using global inconsistency and local inconsistency. Both pooled odds ratio, along with 95% credible interval (CI) and the surface under the cumulative ranking curve values were calculated to assess the outcomes, paired comparisons of efficacy and safety of five treatments. Results: Nine peer-reviewed randomized controlled trials, comprising 1674 patients in recent 10 years, were included. Heterogeneity tests showed no statistical significance, and a consistency model was chosen, respectively. The order of surface under the cumulative ranking curve values for efficacy was as follows: PCNL (79.4), MPCNL (75.2), UMPCNL (66.3), RIRS (29), and eSWL (0). For safety: eSWL (84.2), UMPCNL (82.2), RIRS (52.9), MPCNL (16.6), and PCNL (14.1). Conclusion: In the current study, all five treatments are both effective and safe. Many factors must be considered to choose surgical treatments for LC stones 20 mm or less; the results that we separate conventional PCNL into PCNL, MPCNL, and UMPCNL make the questions even more controversial. However, relative judgments are still needed to be used as reference data in clinical management. For efficacy, PCNL&gt;MPCNL&gt;UMPCNL&gt;RIRS&gt;ESWL, ESWL is statistically inferior to the other four treatments, respectively. RIRS is statistically inferior to PCNL and MPCNL, respectively. For safety, ESWL&gt;UMPCNL&gt;RIRS&gt;MPCNL&gt;PCNL, ESWL is statistically superior to RIRS, MPCNL, and PCNL, respectively. RIRS is statistically superior to PCNL. We cannot reach conclusions about which surgical treatment is the best choice for all patients with LC stones 20 mm or less; therefore, tailored treatments based on individual patients still demand more attention than ever before for both patients and urologists.
Monkeypox clinical symptoms, pathology, and advances in management and treatment options: an update
Authors: Sirwan K. Ahmed, Rabab G.A. El-Kader, Salar O. Abdulqadir, Ardalan J. Abdullah, Nahed A. El-Shall, Deepak Chandran, Abhijit Dey, Talha B. Emran, Kuldeep Dhama
Journal: International Journal of Surgery (2023)

Ahmed, Sirwan K. BSc; El-Kader, Rabab G.A. PhD; Abdulqadir, Salar O. MSc; Abdullah, Ardalan J. MSc; El-Shall, Nahed A. PhD; Chandran, Deepak MVSc; Dey, Abhijit PhD; Emran, Talha B. PhD; Dhama, Kuldeep PhD Author Information
Advancements in cutting-edge cancer treatments using nanotechnology
Authors: Abdullahi T. Aborode, Emmanuel A. Fajemisin, Babatunde Obadawo, Athanasios Alexiou, Nobendu Mukerjee, SrutiBagchi Ghosh, Arabinda Ghosh, Rohit Sharma
Journal: International Journal of Surgery (2023)

Aborode, Abdullahi T. MSc; Fajemisin, Emmanuel A. MSc; Obadawo, Babatunde MSc; Alexiou, Athanasios PhD; Mukerjee, Nobendu BSc; Ghosh, SrutiBagchi PhD; Ghosh, Arabinda PhD; Sharma, Rohit PhD Author Information
Childhood tuberculosis outbreak in Africa: is it a matter of concern?
Authors: Olivier Uwishema, Anushree Rai, Aderinto Nicholas, Mortada Abbass, Lama Uweis, Sara Arab, Rayyan El Saleh, Irem Adanur, Daniel Stephen Masunga, Abubakar Nazir
Journal: International Journal of Surgery (2023)

Uwishema, Olivier MD; Rai, Anushree MD; Nicholas, Aderinto MD; Abbass, Mortada MD; Uweis, Lama MD; Arab, Sara MD; El Saleh, Rayyan MD; Adanur, Irem MD; Stephen Masunga, Daniel MD; Nazir, Abubakar MBBS Author Information
Recent outbreak of Sudan ebolavirus in Uganda and global concern
Authors: Shailesh K. Patel, Aditya Agrawal, Nikhil K. Channabasappa, Jigyasa Rana, Rajat Varshney, Ankush K. Niranjan, Dhruv N. Desai, Megha K. Pandey, Tejinder Kaur, Sita P. Tiwari
Journal: International Journal of Surgery (2023)

Patel, Shailesh K. PhD; Agrawal, Aditya PhD; Channabasappa, Nikhil K. PhD; Rana, Jigyasa PhD; Varshney, Rajat PhD; Niranjan, Ankush K. PhD; Desai, Dhruv N. PhD; Pandey, Megha K. PhD; Kaur, Tejinder PhD; Tiwari, Sita P. PhD Author Information
Dengue virus and its recent outbreaks: current scenario and counteracting strategies
Authors: Ranjit Sah, Abdelmonem Siddiq, Bijaya K. Padhi, Aroop Mohanty, Ali A. Rabaan, Deepak Chandran, Chiranjib Chakraborty, Kuldeep Dhama
Journal: International Journal of Surgery (2023)

Sah, Ranjit MBBS, MD; Siddiq, Abdelmonem BSc; Padhi, Bijaya K. PhD; Mohanty, Aroop MD; Rabaan, Ali A. PhD; Chandran, Deepak PhD; Chakraborty, Chiranjib PhD; Dhama, Kuldeep PhD Author Information
Variability of severe acute respiratory syndrome coronavirus 2 infection in children and adults
Authors: Geetika Verma, Manish Dhawan, AbdulRahman A. Saied, Reetesh Kumar, Ravi P.N. Mishra
Journal: International Journal of Surgery (2023)

Verma, Geetika PhD; Dhawan, Manish MSc; Saied, AbdulRahman A. PhD; Kumar, Reetesh PhD; Mishra, Ravi P.N. PhD Author Information
Artificial intelligence–enabled clinical trials might be a faster way to perform rapid clinical trials and counter future pandemics: lessons learned from the COVID-19 period
Authors: Chiranjib Chakraborty, Manojit Bhattacharya, Kuldeep Dhama, Govindasamy Agoramoorthy
Journal: International Journal of Surgery (2023)

Chakraborty, Chiranjib PhD; Bhattacharya, Manojit PhD; Dhama, Kuldeep PhD; Agoramoorthy, Govindasamy PhD Author Information
Different cellular barriers to RNA therapeutics and strategies to overcome
Authors: Srijan Chatterjee, Manojit Bhattacharya, Govindasamy Agoramoorthy, Chiranjib Chakraborty
Journal: International Journal of Surgery (2023)

Chatterjee, Srijan; Bhattacharya, Manojit Phd; Agoramoorthy, Govindasamy Phd; Chakraborty, Chiranjib Phd Author Information
Emerging hepatitis C virus and neuron-allied neuroviral intertwine and its therapeutic approaches
Authors: Nobendu Mukerjee, Somdatta Y. Chaudhari, Swastika Jha, Shweta Sinha, Shailaja B. Jadhav, Rajib Dhar, Vivek D. Rathod, Rajesh B. Nanaware, Rita D. Chakole, Devesh Sharma, Prince Prashant Sharma, Sonali D. Pawar, Arabinda Ghosh
Journal: International Journal of Surgery (2023)

Mukerjee, Nobendu BSc; Chaudhari, Somdatta Y. MPharm; Jha, Swastika MSc; Sinha, Shweta MSc; Jadhav, Shailaja B. PhD; Dhar, Rajib MSc; Rathod, Vivek D. BTech; Nanaware, Rajesh B. MPharm; Chakole, Rita D. PhD; Sharma, Devesh MSc; Sharma, Prince Prashant PhD; Pawar, Sonali D. MPharm; Ghosh, Arabinda PhD Author Information
Stratification of asthma by lipidomic profiling of induced sputum supernatant
Authors: Joost Brandsma, James P.R. Schofield, Xian Yang, Fabio Strazzeri, Clair Barber, Victoria M. Goss, Grielof Koster, Per S. Bakke, Massimo Caruso, Pascal Chanez, Sven-Erik Dahlén, Stephen J. Fowler, Ildikó Horváth, Norbert Krug, Paolo Montuschi, Marek Sanak, Thomas Sandström, Dominick E. Shaw, Kian Fan Chung, Florian Singer, Louise J. Fleming, Ian M. Adcock, Ioannis Pandis, Aruna T. Bansal, Julie Corfield, Ana R. Sousa, Peter J. Sterk, Rubén J. Sánchez-García, Paul J. Skipp, Anthony D. Postle, Ratko Djukanović
Journal: Journal of Allergy and Clinical Immunology (2023)
<scp>l</scp>‐Arginine‐Modified CoWO<sub>4</sub>/FeWO<sub>4</sub> S‐Scheme Heterojunction Enhances Ferroptosis against Solid Tumor
Authors: Zhuoran Yang, Chunyu Yang, Dan Yang, Ye Zhang, Qingzhu Yang, Fengyu Qu, Wei Guo
Journal: Advanced Healthcare Materials (2023)

Ferroptosis has recently attracted much attention as an anti-tumor therapy. Evidence suggests that ferroptosis can induce oxidative stress and accumulation of lethal lipid peroxides in cancer cells, leading to cell damage. However, unsuitable pH, H2 O2 levels, and high glutathione (GSH) expression in the tumor microenvironment hinder the development of ferroptosis-mediated therapy. In this study, an l-arginine (l-arg)-modified CoWO4 /FeWO4 (CFW) S-scheme heterojunction is strategically designed and constructed for ultrasound (US)-triggered sonodynamic- and gas therapy-induced ferroptosis. CFW not only has excellent Fenton-catalytic activity, outstanding GSH consumption capacity, and excellent ability to overcome tumor hypoxia, but its S-scheme heterostructure can also avoid the rapid combination of electron (e) and hole (h+ ) pairs, thereby enhancing the sonodynamic effects. As a precursor of nitric oxide (NO), l-arg is modified on the surface of CFW (CFW@l-arg) to achieve controlled NO release under US irradiation, thereby enhancing ferroptosis. In addition, poly(allylamine hydrochloride) is further modified on the surface of CFW@l-arg to stabilize l-arg and achieve controllable NO release. Both in vitro and in vivo results demonstrate that such a multifunctional therapeutic nanoplatform can achieve high therapeutic efficacy through sonodynamic and gas therapy-enhanced ferroptosis. This designed oncotherapy nanoplatform provides new inspiration for ferroptosis-mediated therapy.
Long-term human spaceflight and inflammaging: Does it promote aging?
Authors: Miriam Capri, Maria Conte, Erika Ciurca, Chiara Pirazzini, Paolo Garagnani, Aurelia Santoro, Federica Longo, Stefano Salvioli, Patrick Lau, Ralf Moeller, Jens Jordan, Thomas Illig, Maria-Moreno Villanueva, Markus Gruber, Alexander Bürkle, Claudio Franceschi, Jörn Rittweger
Journal: Ageing Research Reviews (2023)

Spaceflight and its associated stressors, such as microgravity, radiation exposure, confinement, circadian derailment and disruptive workloads represent an unprecedented type of exposome that is entirely novel from an evolutionary stand point. Within this perspective, we aimed to review the effects of prolonged spaceflight on immune-neuroendocrine systems, brain and brain-gut axis, cardiovascular system and musculoskeletal apparatus, highlighting in particular the similarities with an accelerated aging process. In particular, spaceflight-induced muscle atrophy/sarcopenia and bone loss, vascular and metabolic changes, hyper and hypo reaction of innate and adaptive immune system appear to be modifications shared with the aging process. Most of these modifications are mediated by molecular events that include oxidative and mitochondrial stress, autophagy, DNA damage repair and telomere length alteration, among others, which directly or indirectly converge on the activation of an inflammatory response. According to the inflammaging theory of aging, such an inflammatory response could be a driver of an acceleration of the normal, physiological rate of aging and it is likely that all the systemic modifications in turn lead to an increase of inflammaging in a sort of vicious cycle. The most updated countermeasures to fight these modifications will be also discussed in the light of their possible application not only for astronauts' benefit, but also for older adults on the ground.
Clinical characteristics and outcome of Mycobacterium chimaera infections after cardiac surgery: systematic review and meta-analysis of 180 heater-cooler unit-associated cases
Authors: Nils Wetzstein, Thomas A. Kohl, Margo Diricks, Silvia Mas-Peiro, Tomas Holubec, Johanna Kessel, Christiana Graf, Benjamin Koch, Eva Herrmann, Maria J.G.T. Vehreschild, Michael Hogardt, Stefan Niemann, Christoph Stephan, Thomas A. Wichelhaus
Journal: Clinical Microbiology and Infection (2023)
Spiritual needs in Denmark: a population-based cross-sectional survey linked to Danish national registers
Authors: Tobias Anker Stripp, Sonja Wehberg, Arndt Büssing, Harold G. Koenig, Tracy A. Balboni, Tyler J. VanderWeele, Jens Søndergaard, Niels Christian Hvidt
Journal: The Lancet Regional Health - Europe (2023)

Spiritual aspects of the human condition may give rise to spiritual pain and suffering, especially in the face of illness or difficult life situations. A growing volume of research documents the effects of religiosity, spirituality, meaning, and purpose on health. In supposedly secular societies, however, spiritual matters are rarely addressed in healthcare. This is the first large scale study to examine spiritual needs in Danish culture, and the largest study on spiritual needs to date. A population-based sample of 104,137 adult (≥18 yrs) Danes were surveyed cross-sectionally (the EXICODE study) and responses were linked to data from Danish national registers. The primary outcome was spiritual needs in four dimensions: religious, existential, generativity, and inner peace. Logistic regression models were fitted to examine the relationship between participant characteristics and spiritual needs. A total of 26,678 participants responded to the survey (25.6%). Of included participants 19,507 (81.9%) reported at least one strong or very strong spiritual need in the past month. The Danes scored highest on inner peace needs, followed by generativity, then existential, and lastly, religious needs. Affiliating as religious or spiritual, regularly meditating or praying, or reporting low health, low life satisfaction, or low well-being increased the odds of having spiritual needs. This study demonstrated that spiritual needs are common among Danes. These findings have important implications for public health policies and clinical care. Care for the spiritual dimension of health is warranted as part of holistic, person-centered care in what we term 'post-secular' societies. Future research should inform how spiritual needs might be addressed in healthy and diseased populations in Denmark and other European countries and the clinical effectiveness of such interventions. The paper was supported by the Danish Cancer Society (R247-A14755), The Jascha Foundation (ID 3610), The Danish Lung Foundation, AgeCare, and the University of Southern Denmark.
Aspartate transferase-to-platelet ratio (APRI): A novel predictor of fatal outcome in patients with SFTS
Authors: Ke Zhang, Yu-Hang Tan, Kang-Li Cao, Chen Gong, Yun-Tao Zhang, Dong-Mei Lv, Yi-Han Ding, Jin-Yu Wang, Yan Zuo, Zhong-Xin Wang, Yuan-Hong Xu
Journal: Journal of Infection (2023)
A retrospective case series of Mohs micrographic surgery and interdisciplinary management of female genital skin cancers: Local recurrence rates and patient-reported outcomes
Authors: Shannon T. Nugent, Leela K. Raj, Nawar A. Latif, Lori Cory, Janos L. Tanyi, Stephen J. Kovach, John P. Fischer, Joshua Fosnot, Ines C. Lin, Jeremy R. Etzkorn, Thuzar M. Shin, Cerrene N. Giordano, H. William Higgins, Joanna L. Walker, Christopher J. Miller
Journal: Journal of the American Academy of Dermatology (2023)
Comparison of glucagon-like peptide-1 receptor agonists and thiazolidinediones on treating nonalcoholic fatty liver disease: A network meta-analysis
Authors: Min Jeong Park, Hayeon Kim, Myeong Gyu Kim, Kyungim Kim
Journal: Clinical and Molecular Hepatology (2023)

Previous studies have revealed that glucagon-like peptide-1 receptor agonist (GLP-1RA) and thiazolidinedione (TZD) can improve nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH). However, comprehensive research comparing the effects of GLP-1RA and TZD is limited. Thus, this study aimed to compare the effects of GLP-1RA and TZD on NAFLD or NASH through a network meta-analysis.
Tongue−Brain‐Transported ZnO Nanoparticles Induce Abnormal Taste Perception
Authors: Aijie Chen, Ruolan Wang, Yiyuan Kang, Jia Liu, Junrong Wu, Yanli Zhang, Yulin Zhang, Longquan Shao
Journal: Advanced Healthcare Materials (2023)

Nanoparticles (NPs) can be transported to the brain, especially through nerves, because of their small size and high biological activity. Previous studies confirmed that zinc oxide (ZnO) NPs can enter the brain through the tongue-brain pathway, but it is unclear whether they will further affect synaptic transmission and brain perception. In this study, it is found that tongue-brain-transported ZnO NPs can cause a decrease in taste sensitivity and taste aversion learning ability, indicating abnormal taste perception. Moreover, the release of miniature excitatory postsynaptic currents, the frequency of action potential release, and the expression of c-fos are decreased, suggesting that the synaptic transmission is reduced. To further explore the mechanism, protein chip detection of inflammatory factors is carried out and it is found that neuroinflammation occurs. Importantly, it is found that neuroinflammation originated from neurons. The JAK-STAT signaling pathway is activated, which inhibits the Neurexin1-PSD95-Neurologigin1 pathway and c-fos expression. Blocking the activation of the JAK-STAT pathway prevents neuroinflammation and the reduction in Neurexin1-PSD95-Neurologigin1. These results indicate that ZnO NPs can be transported by the tongue-brain pathway and lead to abnormal taste perception by neuroinflammation-induced deficits in synaptic transmission. The study reveals the influence of ZnO NPs on neuronal function and provides a novel mechanism.
Mitochondrial Calcium Ion Nanogluttons Alleviate Periodontitis via Controlling mPTPs
Authors: Ping He, Fengyi Liu, Mingzheng Li, Mingxing Ren, Xu Wang, Yangjia Deng, Xianghao Wu, Yuzhou Li, Sheng Yang, Jinlin Song
Journal: Advanced Healthcare Materials (2023)

Abstract The mitochondrial permeability transition (mPT) directly affects mitochondrial function in macrophages. Under inflammatory conditions, mitochondrial calcium ion (mitoCa 2+ ) overload triggers the persistent opening of mPT pores (mPTPs), further aggravating Ca 2+ overload and increasing reactive oxygen species (ROS) to form an adverse cycle. However, there are currently no effective drugs targeting mPTPs to confine or unload excess Ca 2+ . It is novelly demonstrated that the initiation of periodontitis and the activation of proinflammatory macrophages depend on the persistent overopening of mPTPs, which is mainly triggered by mitoCa 2+ overload and facilitates further mitochondrial ROS leakage into the cytoplasm. To solve the above problems, mitochondrial‐targeted “nanogluttons” with PEG‐TPP conjugated to the surface of PAMAM and BAPTA‐AM encapsulated in the core are designed. These nanogluttons can efficiently “glut” Ca 2+ around and inside mitochondria to effectively control the sustained opening of mPTPs. As a result, the nanogluttons significantly inhibit the inflammatory activation of macrophages. Further studies also unexpectedly reveal that the alleviation of local periodontal inflammation in mice is accompanied by diminished osteoclast activity and reduced bone loss. This provides a promising strategy for mitochondria‐targeted intervention in inflammatory bone loss in periodontitis and can be extended to treat other chronic inflammatory diseases associated with mitoCa 2+ overload.
Airway transcriptome networks identify susceptibility to frequent asthma exacerbations in children
Authors: Kieran J. Phelan, Kimberly A. Dill-McFarland, Arjun Kothari, R. Max Segnitz, Jeff Burkle, Brittany Grashel, Seth Jenkins, Daniel Spagna, Lisa J. Martin, David B. Haslam, Jocelyn M. Biagini, Maninder Kalra, Karen S. McCoy, Kristie R. Ross, Daniel J. Jackson, Tesfaye B. Mersha, Matthew C. Altman, Gurjit K. Khurana Hershey
Journal: Journal of Allergy and Clinical Immunology (2023)