tbuckley/cabot-search huggingface dataset:
AB0240 CORRELATION BETWEEN KLOTHO LEVELS AND ANKLE-BRACHIAL INDEX IN FEMALE RHEUMATOID ARTHRITIS PATIENTS
Authors: A. Alvarez de Cienfuegos, J. A. García-Gómez, M. A. González-Gay, N. Ortego
Journal: Annals of the Rheumatic Diseases (2022)

Background Rheumatoid arthritis (RA) that is a chronic systemic and disabling disease affecting 0.5–1% of the general population, have been strongly linked to accelerated aterosclerosis and increased incidence of cardiovascular events and mortality. Klotho is a membrane-bound or soluble antiaging protein, whose protective activity is essential for a proper function of many organs. The association between serum Klotho and the classic risk factors, as well as the clinical history of cardiovascular disease, was also shown. There are a lot of evidences that Klotho deficiency correlates with the occurrence and development of coronary artery disease, atherosclerosis, myocardial infarction, and left ventricular hypertrophy. Objectives The aim of this study was to examine the possible association between serum Klotho levels and ankle-brachial index in women with RA. Methods This cross-sectional study was performed in Vega-Baja Hospital (Orihuela-Spain) from November 2016 to May 2018. We prospectively enrolled 63 consecutive female patients affected by RA and followed at the Vega-Baja Hospital (Orihuela-Spain). All patients included in this study had normal serum creatinine (Cr) levels and met the 2010 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) criteria for RA. Ankle-brachial index were evaluated using a BIDOP model ES-100V3 vascular screening system (Hadeco, Inc, Kawasaki, Japan). Serum Klotho (Elabscience, USA) was analysed using ELISA. Results Our study included 63 female patients, with a mean (SD) age of 53 ± 8 years. The majority were Caucasian (90.5%). The mean duration of RA was 8.5 ± 5.8 years. Mean 28-joint disease activity scores (DAS28) based on erythrocyte sedimentation rate (ESR) indicated low disease activity 3.0 ± 1.3. The mean of the health assessment questionnaire (HAQ) was 0.75 ± 0.67. Mean serum Klotho levels were 4.68 ± 1.14 ng/mL. The mean value of the ankle-brachial index was 1.14 ± 0.1. Lower serum Klotho levels were significantly associated with a lower ankle-brachial index (r 0.27, p&lt;0.05). Conclusion Decreased serum Klotho levels are associated with peripheral arterial disease in women with RA. References [1]Kozera L, Andrews J, Morgan AW. Cardiovascular risk and rheumatoid arthritis—the next step: differentiating true soluble biomarkers of cardiovascular risk from surrogate measures of inflammation. Rheumatology. 2011; 50:1944-1954. [2]Hu MC, Shi M, Cho HJ, Adams-Huet B, Paek J, Hill K, et al. Klotho and phosphate are modulators of pathologic uremic cardiac remodeling. J Am Soc Nephrol. 2015; 26(6):1290–1302. [3]Olejnik A, Franczak A, Krzywonos-Zawadzka A, KaBuhna-Oleksy M, Bil-Lula I. The Biological Role of Klotho Protein in the Development of Cardiovascular Diseases. Biomed Res Int. 2018; 2018:5171945. Disclosure of Interests None declared
AB1217 CHRONIC LOW BACK PAIN AND MUSCULAR HYDATIDOSIS: THE TREE THAT HIDES THE FOREST
Authors: R. Ben Aissa, M. A. Khlif, S. Boussaid, H. Eleuch, K. Zitouna, M. Barsaoui
Journal: Annals of the Rheumatic Diseases (2022)

Background Hydatid cyst disease is a zoonosis caused by Echinococcus granulosus . The parasite implants most commonly in the liver and lung. Musculoskeletal involvement is rare. Objectives To describe clinical and imaging presentation of spinal and muscular hydatidosis. Methods We report two cases of chronic low back pain caused by hydatid cyst. Results Case 1: A 36-year-old woman living in a rural area presented with a three-years history of lumbar back pain complicated with right-sided sciatica and intermittent episodes of urinary retention. The pain was gradually worsening and resisting conventional analgesics and physiotherapy. On physical examination, she had tenderness of the lumbar back and left sacroiliac joint with limited mobility. Biological findings were normal and X-ray features showed osteolytic lesions of L5 left side. The magnetic resonance imaging (MRI) results revealed septated multilocular hydatic cysts with high signal intensity in T2-weighted images and low signal intensity in T1-weighted images along L5 and the sacrum left side. Serological test for hydatid disease was positive. The treatment was wide surgical resection combined with Albendazole (400 mg/day) for one year postoperatively. Case 2: A 62-year-old woman presented with a one-year history of lumbar back pain with gait instability and right flank swelling. Biological findings and X-ray features were normal. The patient first was explored by uro-CT to assess for nephrolithiasis and it revealed retro and sub-peritoneal two multilocular hydatic cysts (60x80mm, 36x35mm) along right iliopsoas and adductor muscles. MRI showed same cystes described on the CT (Figure 1). No other localizations were detected. The diagnosis of hydatidosis was confirmed perioperatively after visualization of the cyst. Wide resection was performed but signs of recurrence were detected by MRI at the 3-month follow-up. Figure 1. MRI showing two multilocular cysts along right iliopsoas (white arrow) and adductor muscles (black arrow). Conclusion In endemic areas, bone and muscle involvement from echinococcosis should be considered during clinical investigation of chronic lumbar pain. Disclosure of Interests None declared
Augmented neutralisation resistance of emerging omicron subvariants BA.2.12.1, BA.4, and BA.5
Authors: Prerna Arora, Amy Kempf, Inga Nehlmeier, Sebastian R Schulz, Anne Cossmann, Metodi V Stankov, Hans-Martin Jäck, Georg M N Behrens, Stefan Pöhlmann, Markus Hoffmann
Journal: The Lancet Infectious Diseases (2022)
AB0578 C-REACTIVE PROTEIN TO ALBUMIN RATIO IN BEHÇET’S DISEASE
Authors: A. Ünal Engİnar
Journal: Annals of the Rheumatic Diseases (2022)

Background Behcet’s Disease (BD) is a systemic variable vessel vasculitis that involves the skin, mucosa, joints, eyes, vascular, nervous and the gastrointestinal system. Objectives There is no specific laboratory test for the diagnosis and following of Behçet’s disease (BD).This study aims to evaluate the relationship between C-reactive protein (CRP)/albumin ratio (CAR) and other hematological parameters in BD. Methods A total of 200 participants (100 BD patients and 100 healthy control) were recruited from the rheumatology outpatient clinic in this cross-sectional study. The laboratory data were obtained from the hospital database. Laboratory tests were conducted to measure complete blood count, erythrocyte sedimentation rate (ESR), CRP, albümin, CAR, neutrophil to lymphocyte ratio (NLR), and platelet to lymphocyte ratio (PLR). Laboratory findings of BD patients and healthy controls were compared and evaluated. BD Activity scores (BDCAF/Behcet’s Disease Current Activity Form) were calculated. The inclusion criteria were fulfilling the international diagnostic criteria of BD, being over 18 years and accepting to participate in the study. Results In the BD group, there were 42 male and 58 female participants with a mean age of 42.49 ± 13.15 and in the healthy control group, 44 male and 56 female participants with a mean age of 44.90 ± 10.98. The age and gender distributions of the groups were as similar (P: 0.16 and P: 0.78, respectively). NLR, CRP, ESR and CAR values were significantly higher in patient group than in the healthy controls (respectively p:0.022, p: 0,013, p:0.001, p:0,011). BDCAF score varied between 0 and 4 with a mean of 1.55± 0.64. A statistically significant correlation was observed between BDCAF and CRP, ESR and CAR (p&lt;0.0001). Conclusion In this study, a significant correlation was found between CAR and BDCAF, NLR, CRP, albumin and ESR. CAR can be useful in the diagnosis and following of BD patients. However, further studies are needed. References [1]Yazici, H., Seyahi, E., Hatemi, G., &amp; Yazici, Y. (2018). Behçet syndrome: a contemporary view. Nature Reviews Rheumatology, 14(2), 107-119. [2]Kim, M., Park, Y. G., &amp; Park, Y. H. (2021). C-reactive protein/albumin ratio as an indicator of disease activity in Behçet’s disease and human leukocyte antigen-B27-associated uveitis. Graefe’s Archive for Clinical and Experimental Ophthalmology, 1-8. [3]Bozkurt, E., Muhafiz, E., Sengul, D., Uçak, T., &amp; Atum, M. (2020). Can the CRP/albumin Ratio be Used as a New Indicator of Activation in Patients with Uveitis?. Ocular immunology and inflammation, 1-6. [4]Hou, C. C., &amp; Guan, J. L. (2021). Risk factors of disease activity in patients with Behçet’s syndrome. Clinical Rheumatology, 40(4), 1465-1471. Disclosure of Interests None declared
POS1176 EFFECT OF LIFESTYLE CHANGE ON INCIDENT GOUT: A NATIONWIDE POPULATION-BASED COHORT OF YOUNG MEN
Authors: Y. Eun, K. D. Han, S. W. Lee, S. Y. Kang, S. Lee, H. S. Cha, E. M. Koh, H. Kim, J. Lee
Journal: Annals of the Rheumatic Diseases (2022)

Background Although gout in young people is increasing, studies on the risk factors for gout in these people are lacking. Objectives In this study, we explored the risk factors of gout and effect of lifestyle change on the development of gout in large nationwide population-based cohort of young men. Methods Between 2009–2012, men aged 20–39 years who participated in two national health examinations at 2-year intervals were included in the study. The outcome was occurrence of gout, which was defined using the diagnosis code of gout (ICD-10 code M10) in the claims database. Cox proportional hazard model was used to evaluate the association between incident gout and baseline factors or lifestyle change. Results A total of 1,977,849 subjects were included in the study, and the mean follow-up period was 5.5 ± 4.3 years. Gout occurred in 38,839 subjects (incidence rate = 3.59/1,000 person-years). A high body mass index, alcohol drinking, and comorbidities such as hypertension, diabetes mellitus, and hyperlipidemia were associated with an increased risk of gout. Among lifestyle factors, change in obesity had the greatest impact on gout, followed by drinking. Development of obesity increased the risk of gout by 1.75 times (95% CI 1.68–1.81), and recovery from obesity decreased the risk of gout by 40% (aHR 0.60, 95% CI 0.57–0.64). Heavy drinking increased the risk of gout by 38% (aHR 1.38, 95% CI 1.33–1.43), and stopping heavy drinking decreased the risk of gout by 11% (aHR 0.89, 95% CI 0.85–0.94). The effect of obesity on gout was evident in the younger age group, and the effect of heavy drinking on gout was weak in the severely obese group. Conclusion Obesity and heavy drinking in young men are important modifiable risk factors for gout. Therefore, the management of these risk factors in young men should be emphasized. Disclosure of Interests None declared
AB0670 Mixed connective tissue disease in a tertiary care hospital
Authors: L. Mendez Diaz, R. J. Gil Velez, I. Madroñal García
Journal: Annals of the Rheumatic Diseases (2022)

Background Mixed connective tissue disease is a rare syndrome characterized by the appearance of Raynaud’s phenomenon, arthritis and edema in the hands, muscle weakness, dysphagia and dyspnea;It consists of the mixture of symptoms present in other collagen diseases such as erythematosus systemic lupus, scleroderma, polymyositis and rheumatoid arthritis, but without meeting diagnostic criteria for any of them. Objectives -Describe the clinical characteristics of a cohort of patients diagnosed with mixed connective tissue disease. -Study the evolution of these patients to other inflammatory autoimmune diseases. Disease conversion was defined as the appearance of new symptoms and autoantibodies compatible with another rheumatic disease. Methods Retrospective descriptive study of patients treated at our Hospital (2010-2020) by the Rheumatology and Internal Medicine service with a diagnosis of mixed connective tissue disease. The data was obtained through the review of medical records. Results 63 patients were included, of whom 56 were women (88%) and 7 men (12%). The average age of diagnosis is 37 +/- 13 years. At the end of the period, only 36.5% (23 patients) met criteria for mixed connective tissue disease. Of the 40 patients (65.5%) who underwent conversion to another autoimmune collagen disease, 65% (26 patients) met the criteria for systemic lupus erythematosus, 22.5% (9 patients) met the criteria for systemic sclerosis, 5% (2 patients) met criteria for rheumatoid arthritis and 7.5% (3 patients) for other systemic collagen pathologies. Of the initial sample of patients, 15 patients (23.8%) developed secondary Sjögren’s disease. Regarding symptoms, in order of frequency, 50 patients (79.3%) of the sample presented myalgia, 39 patients (61.9%) sclerodactyly, 30 patients (47.61%) presented Raynaud’s phenomenon, 10 patients (15.87%) esophageal alterations and 4 patients (6.3%) presented interstitial lung disease. Regarding treatment, 60% of the sample used corticosteroids, 52% hydroxychloroquine, 31.7% methotrexate, 23.8% mycophenolate, and 1.6% required rituximab. In the analyzed period, 2 patients died (3.17%), none as a result of their rheumatic disease. Conclusion The results obtained are consistent with those reported in the medical literature. The majority of patients with mixed connective tissue disease ended up progressing to another autoimmune collagen disease, mainly systemic lupus erythematosus and systemic sclerosis. According to our studies, stricter classification criteria would be convenient. However, more powerful and longer-term studies are needed to be able to draw conclusive conclusions. Disclosure of Interests None declared
POS0446 Arp2/3 AS A Lasp1 INTERACTION PARTNER REGULATES CELL-TO-CELL CONTACT FORMATION OF FIBROBLAST-LIKE SYNOVIOCYTES IN RA
Authors: D. Beckmann, A. Krause, U. Hansen, H. Kiener, J. Kremerskothen, H. Pavenstädt, T. Pap, A. Korb-Pap
Journal: Annals of the Rheumatic Diseases (2022)

Background In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLS) undergo a stable transformation resulting in an aggressive phenotype mediating cartilage damage by increased levels of adhesion molecules. In this context, Lasp1 and the Arp2/3 complex are of interest because they modulate actin organization and focal adhesion turnover. Objectives In this study, the effects of Arp2/3 on cadherin-11 mediated cell-to-cell contact formation have been investigated using the arthritic hTNFtg mouse model. Methods Expression levels of Lasp1 and Arp2/3 protein complex were investigated in synovial tissue of wild type (wt) and hTNFtg hind paws by immunohistochemistry. Primary FLS were analysed, respectively and co-immunoprecipitation experiments were performed. In addition, lasp1 -/ - mice were interbred with hTNFtg animals and offspring were evaluated for disease progression and joint destruction. To further study the role of Arp2/3 in the function of the cadherin-11 adhesion complex, the effects of an Arp2/3 inhibitor (CK666) on cell-to-cell contact formation in FLS derived from hTNFtg and lasp1 -/ - hTNFtg mice were investigated by stainings. To assess signaling pathway activation, cells were stimulated with the growth factor PDGF. Results Upregulated Lasp1 levels were found in synovial tissue and FLS of hTNFtg compared to wt mice. Assays showed that Arp2/3 is part of the adherens junction (AJ) machinery in FLS although Arp2/3 expression levels were not changed between the genotypes. In vivo evaluation of lasp1 -/ - hTNFtg mice revealed a milder arthritis score, less cartilage degradation and reduced FLS attachment to articular cartilage compared to hTNFtg mice. In vitro , the loss of Lasp1 led to clear alterations in AJ arrangement indicated by altered β-catenin pattern. As expected, β-catenin expression was mainly located at adhesion sites between adjacent cells. In hTNFtg FLS, these structures were characterized by a zipper-like pattern. In contrast, these structures were disrupted in lasp1 -/ - hTNFtg FLS. Interestingly, CK666 induced zipper-like structures in hTNFtg FLS comparable to the pattern found in lasp1 -/ - hTNFtg cells. Furthermore, lasp1 -/ - hTNFtg FLS showed decreased Src phosphorylation following PDGF stimulation in comparison to hTNFtg FLS. Conclusion Lasp1 represents an interesting target involved in RA-caused joint destruction, because its loss results in significantly reduced cartilage destruction and altered FLS contacts mediated by Arp2/3. Disclosure of Interests None declared.
POS1003 IMPACT OF ELECTRONIC MEDICAL RECORD MANAGEMENT SYSTEM ON DISEASE ACTIVITY AND FREQUENCY OF OUTPATIENT VISITS IN PATIENTS WITH ANKYLOSING SPONDYLITIS
Authors: H. H. Chen
Journal: Annals of the Rheumatic Diseases (2022)

Background We developed an electronic medical record management system (EMRMS) at the Taichung Veterans General Hospital (TCVGH) to assess ASDAS by integrating patient reported outcomes (assessed by an independent nurse) and ESR/CRP for AS patients. We aimed to investigate the impact of the EMRMS on frequency and time of outpatient visits. Objectives To investigate the impact of the EMRMS on frequency and time of outpatient visits. Methods We identified 652 AS patients who were followed up for at least one year before and after the first ASDAS assessment. We compared the number of outpatient visit and average visit time within one year before and after the initial ASDAS assessment. We identified 201 AS patients who received ≥ 3 continuous ASDAS assessment with an interval of 3 months and compared the 2 nd and 3 rd ASDAS with the first ASDAS. Results The annual number of outpatient visits was increased after starting ASDAS assessment (5.8±3.5 vs. 5.4±3.4, p&lt;0.001), in particular among those whose initial ASDAS-CRP was &gt; 3.5 (8.7±4.0 vs. 5.7±3.4, p&lt;0.001). The average visit time was shortened within one year after ASDAS assessment (8.7±3.8 vs. 9.2±4.4 minutes, p = 0.030), especially among patients whose ASDAS-CRP was &lt;1.3 (8.5±3.3 vs. 9.2±4.2 minutes, p=0.022). Among patients who received at least 3 ASDAS assessments, the 3 rd ASDAS-CRP was significantly lower than the 1 st ASDAS-CRP (1.5±0.8 vs. 1.6±0.8, p=0.040). Conclusion EMRMS may increase the frequency of ambulatory visits for AS patiets with very high disease activity and decrease the visit time for AS patients with inactive disease. Continual ASDAS assessment may help control disease activity of AS patients. Disclosure of Interests None declared
POS0557 ASSOCIATION BETWEEN ANTI CITRULLINATED PEPTIDES ANTIBODIES, BONE MINERAL DENSITY, AND FRACTURE RISK IN RHEUMATOID ARTHRITIS PATIENTS
Authors: O. Lamkhanat, H. Azzouzi, I. Linda
Journal: Annals of the Rheumatic Diseases (2022)

Background Recent literature reported Anti Citrullinated Peptides Antibodies (ACPA) associated with bone loss in patients with rheumatoid arthritis (RA). Objectives To study if ACPA positivity was associated with bone mineral density and the ten-year probability of fracture (FRAX) in RA patients. Methods We performed a cross-sectional study on patients with RA diagnosis according to ACR-EULAR 2010 classification recruited from January 2021 till January 2022. Patients aged from 40 to 90 years were included. Those with prior cancer, hyperparathyroidism, hyperthyroidism, diabetes, chronic kidney disease, and cirrhosis were excluded. Clinical data and results of laboratory tests were collected simultaneously with bone mineral density (BMD) acquisition. The latter was measured with dual-energy X-ray absorptiometry (DXA). BMD of the femoral neck (FN) was used to calculate the ten-year probability of major osteoporotic fracture (FRAX MOF) and femoral neck risk fracture (FRAX FN). Patients were grouped by positivity or quartile of ACPA level (I-IV). We used logistic regression, parametric and non-parametric tests for statistical analysis with SPSS20. Results Eighty-two RA patients were enrolled. In total, 69 (84.1%) were women, 16 (19.5%) reported a previous personal fracture, and 37 (45.1%) had osteoporosis. Seventy-two (87.8%) patients were ACPA positive. The mean body mass index (BMI; kg/m 2 ) and lumbar spine BMD in ACPA-positive patients were 25.44 ± 4.79 and 0.791 ± 0.138, respectively. We found a significant association between ACPA positivity and BMI over 25 (p = 0.01; OR = 0.132 [1.527; 6.785]). Comparison between ACPA positive/negative patients did not find a significant difference for osteoporosis, history of fracture, DAS28CRP, lumbar spine BMD, FN BMD, FRAX MOF BMD, and FRAX FN BMD. Lumbar spine BMD was significantly different (p = 0.035) among subgroups of ACPA levels, whereas there was no significant difference for FRAX MOF, FRAX FN, and FN BMD. Conclusion In this study, lumbar spine BMD was significantly different among subgroups of ACPA levels. Disclosure of Interests None declared.
Polymer selection impacts the pharmaceutical profile of site-specifically conjugated Interferon-α2a
Authors: Niklas Hauptstein, Paria Pouyan, Kevin Wittwer, Gizem Cinar, Oliver Scherf-Clavel, Martina Raschig, Kai Licha, Tessa Lühmann, Ivo Nischang, Ulrich S. Schubert, Christian K. Pfaller, Rainer Haag, Lorenz Meinel
Journal: Journal of Controlled Release (2022)
AB0699 DESCRIPTIVE STUDY OF A COHORT OF PATIENTS WITH DERMATOMYOSITIS IN A TERTIARY HOSPITAL.
Authors: R. J. Gil Velez, L. Mendez Diaz, I. Madroñal García
Journal: Annals of the Rheumatic Diseases (2022)

Background Idiopathic chronic inflammatory myopathies (IICM) are a heterogeneous group of rare diseases characterized by skeletal muscle inflammation. The incidence ranges from 1 to 60 new cases per 1,000,000 habitants/year and the prevalence between 2.4 and 34 per 100,000 habitants.The main symptoms are muscle weakness, which may be accompanied by contractures, pain, cramps or stiffness and symptoms related to localized disease in skin, lungs and other organs. Increased muscle enzymes and a myopathic pattern are seen on electromyography. Definitive diagnosis is made by muscle biopsy with lymphocyte infiltrates, necrosis and regeneration of muscle fibers. Objectives Description of the clinical characteristics of a cohort of patients diagnosed with dermatomyositis together with the study of the evolution: organic involvement, specific autoantibody profile and treatments performed in this population. Methods Descriptive study of a cohort of patients diagnosed with dermatomyositis at the Rocio Virgin University Hospital (Seville), during 2010-2022, obtained from medical records. Results Sixty patients were included, including 17 (28.3%), 43 (71.7%) women, whose mean age 58 +/-12 years, and mean time of diagnosis was 5 years. Among the types of inflammatory myopathies: dermatomyositis 37 (61.6%), amyopathic DM 6 (10%), antisynthetase syndrome 5 (8.3%), paraneoplastic 1 (1.6%) and rapidly progressive MDA5 7 (11.6%) Among the most frequent affections: pulmonary 20 (34%) being the most frequent NINE pattern 9 (45%), NIU 8 (40%); esophageal 20 (34%), cardiological 12 (20%). Among the specific antibodies: ANA positive 24(40%), Ro 3(5%), La4(6.6%),JO1 6(10%),PL7 1(1.6%)PL12 1(1.6%),Mi2 1(,1.6%),SAE 3(5%),NXP2 2(3.3%),SRP 1(1.6%),MDA5 7(11.6%) and HMG-CoA 3(5%). Among the diagnostic tests MRI/electromyogram were positive 63% and 56.6% respectively. Biopsy was positive in 72%(42) of cases. The drugs used to induce remission were: cyclophosphamide 36 (60%), rituximab 12 (20%), immunoglobulins 6 (10%) and mycophenolate mofetil 6 (10%). As for the maintenance of remission: mycophenolate 54 (90%), methotrexate 3 (5%) and azathioprine 3 (5%). There was only one exitus caused by cardiac arrhythmia. Conclusion The results obtained have been in accordance with the published literature, with a clear association between specific autoantibodies and the different muscular and extramuscular manifestations. Among the remission inducers, the most frequent regimen used in our setting is the use of cyclophosphosmide/ rituximab and maintenance of remission with azathioprine and mycophenonate. More studies on new therapeutic options are needed. Disclosure of Interests None declared