tbuckley/cabot-search huggingface dataset:
Solid‐Phase Screening and Synthesis of Molecularly Imprinted Nanoparticles for Selective Recognition and Detection of Brain Natriuretic Peptide
Authors: Ziying Zhang, Liang Ma, Hui Yuan, Ziman Chen, Yongqin Lv
Journal: Advanced Healthcare Materials (2023)

Abstract A new recognition method is explored for the rapid detection of B‐type natriuretic peptide (BNP) based on the rational design and solid‐phase synthesis of molecularly imprinted nanoparticles (nanoMIP) encapsulated with carbon dots. The nanosized magnetic template is first prepared by attaching the epitope of BNP on amino‐functionalized magnetic carriers. High‐dilution polymerization of monomers in the presence of magnetic template generates lightly crosslinked imprinted nanoparticles. To obtain the optimal MIP formulation, a new combinatorial screening approach is developed by a competitive fluorescence assay using the magnetic template. The resultant nanoMIP exhibits high affinity and selectivity toward BNP with an equilibrium dissociation constant ( K D ) of ≈10 −11 m . The proposed assay allows fast BNP detection within ≈7 min with a linear range of its concentration from 0.25 to 5000 pg mL −1 and a limit of detection of 0.208 pg mL −1 (S/N = 3). To demonstrate its practicability in clinical diagnosis, unknown real serum samples from 160 individuals are analyzed and the relative standard deviation is less than 4.43%. Compared with the routine electrochemiluminescence detection method that is widely used in hospital, the relative error is less than 4.98% and the correlation coefficient is 0.994.
The accidental discovery of pancreatic ductal adenocarcinoma on percutaneous cholangioscopy through a T-tube tract
Authors: Wengang Zhang, Ningli Chai, Yaqi Zhai, Huikai Li, Shengzhen Liu, Fei Gao, Enqiang Linghu
Journal: Endoscopy (2023)

A 58-year-old man with pancreatitis underwent contrast-enhanced computed tomography and was found to have gallstones. He therefore underwent cholecystectomy and intraoperative choledochoscopy at his local hospital, during which some sediment-like stones were removed from the common bile duct (CBD), and a T-tube was left in place. Postoperative cholangiography through the T-tube showed obstruction in the distal CBD ([Fig. 1]) and he was therefore transferred to our center for further diagnosis and treatment.
Successful retrieval of migrated and embedded fully covered self-expanding metal stent using “snare and lithotripter” technique
Authors: Michael Kin-kong Li, Ka-kin Sze, Terence Yee-hong Tong
Journal: Endoscopy (2023)

A 52-year-old woman with benign perihilar left intrahepatic duct stricture underwent repeated stricture dilation and multiple plastic stent placements. Subsequently, an 8 mm × 12 cm multi-hole fully covered self-expanding metal stent (MH-FCSEMS) was inserted. Six months later, she had abnormal liver biochemistry. Endoscopic retrograde cholangiography (ERC) revealed supra-papillary stent migration and tissue ingrowth at the proximal end ([Fig. 1]). Cholangioscopy (SpyGlass System; Boston Scientific Corp., Marlborough, Massachusetts, USA) showed that the stent lumen was obliterated by stone cast ([Fig. 2]). Stent removal with forceps and snare failed. The patient was referred to our center for a second opinion.
Clinical Trial of Autologous Cultivated Limbal Epithelial Cell Sheet Transplantation for Patients with Limbal Stem Cell Deficiency
Authors: Yoshinori Oie, Seiichiro Sugita, Shunji Yokokura, Toru Nakazawa, Daisuke Tomida, Yoshiyuki Satake, Jun Shimazaki, Yuko Hara, Atsushi Shiraishi, Andrew J. Quantock, Takahiro Ogasawara, Masukazu Inoie, Kohji Nishida
Journal: Ophthalmology (2023)
Eating Disorders in Boys and Men
Authors: Tiffany A. Brown, Pamela K. Keel
Journal: Annual Review of Clinical Psychology (2023)

While boys and men have historically been underrepresented in eating disorder research, increasing interest and research during the twenty-first century have contributed important knowledge to the field. In this article, we review the epidemiology of eating disorders and muscle dysmorphia (the pathological pursuit of muscularity) in boys and men; specific groups of men at increased risk for eating disorders; sociocultural, psychological, and biological vulnerability factors; and male-specific assessment measures. We also provide an overview of current research on eating disorder and muscle dysmorphia prevention efforts, treatment outcomes, and mortality risk in samples of boys and men. Priorities for future research are including boys and men in epidemiological studies to track changes in incidence, identifying (neuro)biological factors contributing to risk, eliminating barriers to treatment access and utilization, and refining male-specific prevention and treatment efforts.
Recent Progress on Nanomaterial‐Facilitated Electrochemical Strategies for Cancer Diagnosis
Authors: Xueli Yuan, Bowen Lin, Tao Liu, Wei Zhang, Zhenyu Chu, Xiaoping Gu, Zhengliang Ma, Wanqin Jin
Journal: Advanced Healthcare Materials (2023)

Cancer is a malignant disease that endangers human life, especially owing to its high fatality rate; therefore, rapid and accurate early screening is needed to effectively improve the survival rate. Compared with traditional cancer detection methods, electrochemical biosensors that recognize cancer biomarkers in blood have the advantages of low invasiveness, fast diagnosis, and low cost. However, there is always a trade-off between sensitivity and selectivity, which limits the detection of trace amounts of biomarkers produced in the early stages. To address this issue, an increasing number of nanomaterials with simultaneous improvements in both sensitivity and selectivity have recently been reported. In this review, different categories of state-of-the-art electrochemical biosensors and their operating principles are introduced, and their respective advantages and disadvantages are described. Furthermore, the review discusses the existing detection strategies and performance of nanomaterial-based cancer biosensors for biomarker recognition, providing overall guidance for the material selection of different biomarkers. Finally, the main challenges involving existing electrochemical cancer biosensors are evaluated to present the future development prospects of nanomaterials and detection strategies.
AAV vectors applied to the treatment of CNS disorders: Clinical status and challenges
Authors: Lin Kang, Shilin Jin, Jiayi Wang, Zhongyue Lv, Chengqi Xin, Chengcheng Tan, Mengke Zhao, Liang Wang, Jing Liu
Journal: Journal of Controlled Release (2023)

In recent years, adeno-associated virus (AAV) has become the most important vector for central nervous system (CNS) gene therapy. AAV has already shown promising results in the clinic, for several CNS diseases that cannot be treated with drugs, including neurodegenerative diseases, neuromuscular diseases, and lysosomal storage disorders. Currently, three of the four commercially available AAV-based drugs focus on neurological disorders, including Upstaza for aromatic l-amino acid decarboxylase deficiency, Luxturna for hereditary retinal dystrophy, and Zolgensma for spinal muscular atrophy. All these studies have provided paradigms for AAV-based therapeutic intervention platforms. AAV gene therapy, with its dual promise of targeting disease etiology and enabling 'long-term correction' of disease processes, has the advantages of immune privilege, high delivery efficiency, tissue specificity, and cell tropism in the CNS. Although AAV-based gene therapy has been shown to be effective in most CNS clinical trials, limitations have been observed in its clinical applications, which are often associated with side effects. In this review, we summarized the therapeutic progress, challenges, limitations, and solutions for AAV-based gene therapy in 14 types of CNS diseases. We focused on viral vector technologies, delivery routes, immunosuppression, and other relevant clinical factors. We also attempted to integrate several hurdles faced in clinical and preclinical studies with their solutions, to seek the best path forward for the application of AAV-based gene therapy in the context of CNS diseases. We hope that these thoughtful recommendations will contribute to the efficient translation of preclinical studies and wide application of clinical trials.
Endoscopic transpapillary repositioning of a migrated cystic duct tube using a gooseneck snare technique
Authors: Takehiko Koga, Yusuke Ishida, Hiroto Ishikawa, Yukiya Kishimoto, Satoki Kojima, Masayuki Okabe, Fumihito Hirai
Journal: Endoscopy (2023)

A cystic duct tube (C-tube) may be inserted via the cystic duct into the common bile duct (CBD) during cholecystectomy for postoperative percutaneous biliary drainage [1] [2] [3] [4] [5]. Postoperative C-tube migration has been reported in 6.6 % cases [2]. Herein, we describe successful endoscopic repositioning of a migrated C-tube ([Video 1]).
Integrated Cascade Nanozyme Remodels Chondrocyte Inflammatory Microenvironment in Temporomandibular Joint Osteoarthritis via Inhibiting ROS‐NF‐<i>κ</i>B and MAPK Pathways
Authors: Zhongyin Zhang, Lichan Yuan, Yufeng Liu, Ruobing Wang, Yihong Zhang, Yan Yang, Hui Wei, Junqing Ma
Journal: Advanced Healthcare Materials (2023)

Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative joint disease with no complete cure at present. Notably, the inflammatory microenvironment in TMJ OA is modulated by oxidative stress, which impacts cartilage metabolism, chondrocyte apoptosis, inflammatory cytokine release, and extracellular matrix (ECM) synthesis. Thus, it is reasoned that reducing excess reactive oxygen species (ROS) in the chondrocyte microenvironment may be an effective therapeutic strategy for TMJ OA. Recently, cascade nanozymes, including Pt@PCN222-Mn, have been exploited to treat ROS-associated diseases. Nevertheless, cascade nanozymes are not employed for TMJ OA therapy. To fill this gap, it is explored whether the Pt@PCN222-Mn cascade nanozyme could be applied to the treatment of TMJ OA. The in vitro results demonstrate that the Pt@PCN222-Mn nanozyme can inhibit the production of inflammatory factors, the degradation of ECM, and the apoptosis of chondrocytes by inhibiting the ROS-nuclear factor kappa-B (NF-κB_ and mitogen-activated protein kinase signaling pathways. The in vivo results further demonstrate that the Pt@PCN222-Mn nanozyme can delay the progression of TMJ OA in the rat unilateral anterior crossbite model. It is believed that insightful perspectives on the application of nanozymes in TMJ OA will be provided here.
Plasma proteomic signature of fatty liver disease: The Rotterdam Study
Authors: Yasir J. Abozaid, Ibrahim Ayada, Laurens A. van Kleef, Costanza L. Vallerga, Qiuwei Pan, Willem P. Brouwer, M. Arfan Ikram, Joyce Van Meurs, Robert J. de Knegt, Mohsen Ghanbari
Journal: Hepatology (2023)

Fatty liver disease (FLD) is caused by excess fat in the liver, and its global prevalence exceeds 33%. The role of protein expression on the pathogenesis of FLD and accompanied fibrosis and its potential as a disease biomarker is currently not clear. Hence, we aimed to identify plasma proteomics associated with FLD and fibrosis using population-based data.Blood samples were collected from 2578 participants from the population-based Rotterdam Study cohort. The proximity extension assay reliably measured plasma levels of 171 cardiometabolic and inflammatory-related proteins (Olink Proteomics). FLD was assessed by ultrasound, and fibrosis by transient elastography. Logistic regression models quantified the association of plasma proteomics with FLD and fibrosis. In addition, we aimed to validate our results in liver organoids. The cross-sectional analysis identified 27 proteins significantly associated with FLD surpassing the Bonferroni-corrected p <2.92×10 -4 . The strongest association was observed for FGF-21 (β=0.45, p =1.07×10 -18 ) and carboxylesterase 1 (CES1) protein (β=0.66, p =4.91×10 -40 ). Importantly, 15 of the 27 proteins significantly associated with FLD were also associated with liver fibrosis. Finally, consistent with plasma proteomic profiling, we found the expression levels of IL-18 receptor 1 (IL-18R1) and CES1 to be upregulated in an FLD model of 3-dimensional culture human liver organoids.Among the general population, several inflammatory and cardiometabolic plasma proteins were associated with FLD and fibrosis. Particularly, plasma levels of FGF-21, IL-18R1, and CES1 were largely dependent on the presence of FLD and fibrosis and may therefore be important in their pathogenesis.
High‐Precision Synthesis of RNA‐Loaded Lipid Nanoparticles for Biomedical Applications
Authors: Hanjin Seo, Leekang Jeon, Jaeyeong Kwon, Hyomin Lee
Journal: Advanced Healthcare Materials (2023)

Abstract The recent development of RNA‐based therapeutics in delivering nucleic acids for gene editing and regulating protein translation has led to the effective treatment of various diseases including cancer, inflammatory and genetic disorder, as well as infectious diseases. Among these, lipid nanoparticles (LNP) have emerged as a promising platform for RNA delivery and have shed light by resolving the inherent instability issues of naked RNA and thereby enhancing the therapeutic potency. These LNP consisting of ionizable lipid, helper lipid, cholesterol, and poly(ethylene glycol)‐anchored lipid can stably enclose RNA and help them release into the cells’ cytosol. Herein, the significant progress made in LNP research starting from the LNP constituents, formulation, and their diverse applications is summarized first. Moreover, the microfluidic methodologies which allow precise assembly of these newly developed constituents to achieve LNP with controllable composition and size, high encapsulation efficiency as well as scalable production are highlighted. Furthermore, a short discussion on current challenges as well as an outlook will be given on emerging approaches to resolving these issues.
JHEP at a glance (February 2023)
Authors: Wei Shen, Michael Middleton, Guilherme Cunha, Jane Zhou, Claude Sirlin, Joel Lavine, Arun Sanyal, Julie Foucquier, Zobair Younossi, Véronique Miette, Laurent Sandrin, Jérôme Boursier, Joanne Carson, Sebastiano Barbieri, Gail Matthews, Gregory Dore, Behzad Hajarizadeh
Journal: Journal of Hepatology (2023)
Contents
Authors: P Burra, F Tacke, V Ratziu, S Zeuzem, B Sangro, P Angeli, B Koerkamp, P.-A Clavien, W Polak, W Shen, G Middleton, T Cunha, T Delgado, A Wolfson, K Gamst, A Fowler, A Alazraki, M Trout, S Ohliger, M Shah, D Bashir, R Kleiner, B Loomba, A Neuschwander-Tetri, J Sanyal, C Zhou, J Sirlin, A Sanyal, J Foucquier, Z Younossi, S Harrison, P Newsome, W.-K Chan, Y Yilmaz, V De Ledinghen, C Costentin, M.-H Zheng, Wai-Sun Wong, M Elkhashab, R Huss, R Myers, M Roux, A Labourdette, M Destro, C Fournier-Poizat, V Miette, L Sandrin, J Boursier, J Carson, S Barbieri, G Matthews, G Dore, B Hajarizadeh, L Sun, H Feng, I Misumi, T Shirasaki, L Hensley, O González-López, I Shiota, W.-C Chou, P.-Y Ting, J Cullen, D Cowley, J Whitmire, S Lemon, P.-C Tsai, H.-T Kuo, C.-H Hung, K.-C Tseng, H.-C Lai, C.-Y Peng, J.-H Wang, J.-J Chen, P.-L Lee, R.-N Chien, C.-C Yang, G.-H Lo, J.-H Kao, C.-J Liu, C.-H Liu, S.-L Yan, M.-J Bair, C.-Y Lin, W.-W Su, C.-H Chu, C.-J Chen, S.-Y Tung, C.-M Tai, C.-W Lin, C.-C Lo, P.-N Cheng, Y.-C Chiu, C.-C Wang, J.-S Cheng, W.-L Tsai, H.-C Lin, Y.-H Huang, M.-L Yeh, C.-F Huang, M.-H Hsieh, J.-F Huang, C.-Y Dai, W.-L Chung, C.-Y Chen, M.-L Yu, N Chalasani, Y.-J Li, A Dellinger, V Navarro, H Bonkovsky, R Fontana, J Gu, H Barnhart, E Phillips, C Lammert, T.-H Schwantes-An, P Nicoletti, D Kleiner, J Hoofnagle, A Zanetto, F Pelizzaro, E Campello, C Bulato, L Balcar, W Gu, S Gavasso, G Saggiorato, F Russo, M Mandorfer, T Reiberger, J Trebicka, P Simioni, M Senzolo, A Fagan, E Gavis, M Gallagher, T Mousel, B Davis, P Puri, R Sterling, V Luketic, H Lee, S Matherly, R Stravitz, V Patel, A Siddiqui, M Asgharpour, L Fuchs, J Thacker, D Samuel, R Morikawa, N Nakamoto, T Amiya, P.-S Chu, Y Koda, T Teratani, T Suzuki, Y Kurebayashi, A Ueno, N Taniki, K Miyamoto, A Yamaguchi, S Shiba, T Katayama, K Yoshida, Y Takada, R Ishihara, H Ebinuma, M Sakamoto, T Kanai, S.-T Cheng, J.-L Hu, J.-H Ren, H.-B Yu, S Zhong, V Wong, B Kwan Law, W.-X Chen, H.-M Xu, Z.-Z Zhang, X.-F Cai, Y Hu, W.- L Zhang, Q.-X Long, F Ren, H.-Z Zhou, A.-L Huang, J Howell, A Pedrana, S Schroeder, N Scott, L Aufegger, R Atun, R Baptista-Leite, G Hirnschall, E 't Hoen, S Hutchinson, J Lazarus, L Olufunmilayo, R Peck, M Sharma, A Sohn, A Thompson, M Thursz, D Wilson, M Hellard, S.-Y Cai, D Yu, C Soroka, J Boyer, D Chand, F Mohr, H Mcmillan, F Tukov, K Montgomery, A Kleyn, R Sun, S Tauscher-Wisniewski, P Kaufmann, G Kullak-Ublick, T Marjot, A Moon, J Cook, S Abd-Elsalam, C Aloman, M Armstrong, E Pose, E Brenner, T Cargill, M.-A Catana, R Dhanasekaran, A Eshraghian, I García-Juárez, U Gill, P Jones, J Kennedy, A Marshall, C Matthews, G Mells, C Mercer, P Perumalswami, E Avitabile, X Qi, F Su, N Ufere, Y Wong, E Barnes, A Barritt, G Webb
Journal: Journal of Hepatology (2023)
The imperative of arachidonic acid in early human development
Authors: Michael A. Crawford, Andrew J. Sinclair, Barbara Hall, Enitan Ogundipe, Yiqun Wang, Dimitrios Bitsanis, Ovrang B. Djahanbakhch, Laurence Harbige, Kebreab Ghebremeskel, Ivan Golfetto, Therishnee Moodley, Ahmed Hassam, AnnieBelle Sassine, Mark R. Johnson
Journal: Progress in Lipid Research (2023)

We are presenting new evidence on essential fatty acids (EFA) in prenatal human development. We have demonstrated, for the first time, the detailed process of active selection of some fatty acids by the placenta (biomagnification) and rejection of others (bioreduction) and how this strategy is of supreme importance for understanding of the biology of human reproduction. The biomagnification process by the placenta is dominated by arachidonic acid (ArA) and its allies: di-homo-gamma-linolenic acid (DGLA), adrenic acid and ω6 docosapentaenoic acid. Stearic acid is similarly bio-magnified which is likely to provide for the sn-1 position in membrane synthesis. In contrast there is a bioreduction of oleic, linoleic and all ω3 precursors for docosahexaenoic acid (DHA), including eicosapentaenoic acid (EPA). Although DHA is biomagnified, the amplification from mother to fetus is small compared to ArA. We report on the dominant compartmentalisation of ArA from mother to fetal plasma, cell membranes of red cells, mono-nuclear cells, endothelium and the placenta. We conclude that ArA and its allies, play a paramount role in the development of the products of conception. It is plausible that inadequate provision of ArA may be relevant to the neuro-vascular complications of prematurity and neurodevelopmental disorders associated with premature birth. We present evidence of ArA's universal role from an identical arachidonic acid-based strategy observed in contrasting cultures. The dominance of ArA in the prenatal and in post-natal nutritional provision by human milk makes a compelling case for re-evaluation of its role, especially in reproductive biology.
Letter to the Editor: clinical utility of urine DNA for noninvasive detection and minimal residual disease monitoring in urothelial carcinoma
Authors: Kaiwei Yang, Hailong Hu, Junlong Wu, Huina Wang, Zhaoxia Guo, Wei Yu, Lin Yao, Feng Ding, Tao Zhou, Wang Wang, Yunkai Wang, Lei Liu, Jing Guo, Shuaipeng Zhu, Xinhao Zhang, Shanbo Cao, Feng Lou, Yuanjie Niu, Dingwei Ye, Zhisong He
Journal: Molecular Cancer (2023)

Abstract Current methods for the early detection and minimal residual disease (MRD) monitoring of urothelial carcinoma (UC) are invasive and/or possess suboptimal sensitivity. We developed an efficient workflow named urine tumor DNA multidimensional bioinformatic predictor (utLIFE). Using UC-specific mutations and large copy number variations, the utLIFE-UC model was developed on a bladder cancer cohort ( n = 150) and validated in The Cancer Genome Atlas (TCGA) bladder cancer cohort ( n = 674) and an upper tract urothelial carcinoma (UTUC) cohort ( n = 22). The utLIFE-UC model could discriminate 92.8% of UCs with 96.0% specificity and was robustly validated in the BLCA_TCGA and UTUC cohorts. Furthermore, compared to cytology, utLIFE-UC improved the sensitivity of bladder cancer detection ( p &lt; 0.01). In the MRD cohort, utLIFE-UC could distinguish 100% of patients with residual disease, showing superior sensitivity compared to cytology ( p &lt; 0.01) and fluorescence in situ hybridization (FISH, p &lt; 0.05). This study shows that utLIFE-UC can be used to detect UC with high sensitivity and specificity in patients with early-stage cancer or MRD. The utLIFE-UC is a cost-effective, rapid, high-throughput, noninvasive, and promising approach that may reduce the burden of cystoscopy and blind surgery.
Regulatory cells and the effect of cancer immunotherapy
Authors: María Iglesias-Escudero, Noelia Arias-González, Eva Martínez-Cáceres
Journal: Molecular Cancer (2023)

Abstract Several mechanisms and cell types are involved in the regulation of the immune response. These include mostly regulatory T cells (Tregs), regulatory macrophages (Mregs), myeloid suppressor cells (MDSCs) and other regulatory cell types such as tolerogenic dendritic cells (tolDCs), regulatory B cells (Bregs), and mesenchymal stem cells (MSCs). These regulatory cells, known for their ability to suppress immune responses, can also suppress the anti-tumor immune response. The infiltration of many regulatory cells into tumor tissues is therefore associated with a poor prognosis. There is growing evidence that elimination of Tregs enhances anti-tumor immune responses. However, the systemic depletion of Treg cells can simultaneously cause deleterious autoimmunity. Furthermore, since regulatory cells are characterized by their high level of expression of immune checkpoints, it is also expected that immune checkpoint inhibitors perform part of their function by blocking these molecules and enhancing the immune response. This indicates that immunotherapy does not only act by activating specific effector T cells but can also directly or indirectly attenuate the suppressive activity of regulatory cells in tumor tissues. This review aims to draw together our current knowledge about the effect of immunotherapy on the various types of regulatory cells, and how these effects may be beneficial in the response to immunotherapy.
Cre recombinase expression cooperates with homozygous FLT3 internal tandem duplication knockin mouse model to induce acute myeloid leukemia
Authors: Jasmin Straube, Theresa Eifert, Therese Vu, Yashaswini Janardhanan, Rohit Haldar, Björn von Eyss, Leanne Cooper, Claudia Bruedigam, Victoria Y. Ling, Emily Cooper, Ann-Marie Patch, Lars Bullinger, Tina M. Schnoeder, Megan Bywater, Florian H. Heidel, Steven W. Lane
Journal: Leukemia (2023)

Murine models offer a valuable tool to recapitulate genetically defined subtypes of AML, and to assess the potential of compound mutations and clonal evolution during disease progression. This is of particular importance for difficult to treat leukemias such as FLT3 internal tandem duplication (ITD) positive AML. While conditional gene targeting by Cre recombinase is a powerful technology that has revolutionized biomedical research, consequences of Cre expression such as lack of fidelity, toxicity or off-target effects need to be taken into consideration. We report on a transgenic murine model of FLT3-ITD induced disease, where Cre recombinase expression alone, and in the absence of a conditional allele, gives rise to an aggressive leukemia phenotype. Here, expression of various Cre recombinases leads to polyclonal expansion of FLT3ITD/ITD progenitor cells, induction of a differentiation block and activation of Myc-dependent gene expression programs. Our report is intended to alert the scientific community of potential risks associated with using this specific mouse model and of unexpected effects of Cre expression when investigating cooperative oncogenic mutations in murine models of cancer.