tbuckley/cabot-search huggingface dataset:
Optimized mobilization of MHC class I- and II- restricted immunity by dendritic cell vaccine potentiates cancer therapy
Authors: Yingying Shi, Yu Liu, Jiaxin Huang, Zhenyu Luo, Xuemeng Guo, Mengshi Jiang, Xiang Li, Yichao Lu, Xu Liu, Xinyu Shan, Lihua Luo, Jian You
Journal: Theranostics (2022)

Background: The participation of major histocompatibility complex (MHC) in antigen presentation shapes both the breadth and magnitude of specific T cell response.Dendritic cells (DCs) activated with nucleic acid or protein that encodes/incorporates multiple antigenic epitopes elicit MHC class I-and II-biased immunity, respectively.Studies demonstrate that an elevated MHC class I-directed CD8 + cytotoxicity T lymphocyte (CTL) response is able to provide survival benefits to patient with malignant tumor.However, a fully effective cancer therapy must elicit a diverse repertoire of both CD4 + and CD8 + T cell responses, raising demands on a multifaceted activation of the MHC system.Current therapeutic strategies usually lack an orchestrated mobilization of the MHC class I and II responses.Vaccines with little synergistic effect or unmanageable elicitation of the CD4 + and CD8 + T cell immunity usually fail to induce a potent and durable anti-tumor protection.Methods: Here, cationic nanoemulsions (CNEs) complexed with full-length tumor model antigen ovalbumin (OVA) in the form of mRNA or protein were constructed and used as two antigenic platforms to prepare DCs vaccines with tailored MHC participation (i.e., mRNA-DCs and protein-DCs).In exploring a vaccine regimen with optimal tumor suppressing effect, the mixing ratio of mRNA-DCs and protein-DCs was manipulated.Results: Therapeutic DCs vaccines involving both antigenic platforms induced better anti-tumor immunity in murine E.G7-OVA lymphoma model and B16-OVA melanoma model, which can be further augmented upon a meticulous reallocation of the MHC class I and II responses.Conclusion: This work indicated that a simultaneous and coordinated mobilization of the MHCrestricted immunity might potentiate cancer therapy.
Endothelial S1pr2 regulates post-ischemic angiogenesis via AKT/eNOS signaling pathway
Authors: Caixia Zhou, Yashu Kuang, Qinyu Li, Yunhao Duan, Xiuxiang Liu, Jinnan Yue, Xiaoli Chen, Jie Liu, Yuzhen Zhang, Lin Zhang
Journal: Theranostics (2022)

Aims: It is important to understand the mechanism that regulates post-ischemic angiogenesis and to explore a new therapeutic target for an effective improvement of revascularization in peripheral artery disease (PAD) patients.Post-ischemic angiogenesis is a highly orchestrated process, which involves vascular endothelial cells (ECs) proliferation, migration and assembly into capillaries.We found a significant reduction of S1pr2 (sphingosine 1-phosphate receptor 2) in endothelial cells after hindlimb ischemia (HLI).We thus hypothesized that EC-S1pr2 might be involved in the regulation of post-ischemic angiogenesis and blood flow recovery during peripheral arterial disease (PAD).Methods and Results: We generated both EC-specific S1pr2 loss-of-function and S1pr2 gain-of-function mice.Our study showed that EC-specific S1pr2 loss-of-function significantly enhanced post-ischemic angiogenesis and improved blood flow recovery upon femoral artery ligation, whereas the EC-specific S1pr2 gain-of-function severely hindered post-ischemic angiogenesis and reduced blood flow recovery in ischemic limbs.We next identified that S1pr2 inhibited AKT/eNOS signaling pathway, and thus inhibited EC proliferation/migration and angiogenic activity.As expected, pharmacological inhibition of S1pr2 by JTE013 improved post-ischemic angiogenesis and improved blood flow perfusion after femoral artery ligation.Moreover, we developed RGD-peptide magnetic nanoparticles packaging S1pr2-siRNA which specifically targeted ECs and achieved an efficient silencing of S1pr2 expression in ECs in vivo.This EC-targeted strategy to dampen S1pr2 significantly enhanced post-ischemic angiogenesis and boosted blood perfusion after HLI, supplying a novel therapy target for patients with peripheral arterial disease.Conclusions: This present study demonstrates that EC-expressing S1pr2 tightly controls post-ischemic angiogenesis and blood flow perfusion recovery.This research provides a novel strategy for EC-target knockdown of S1pr2 as a new therapeutic intervention for patients with peripheral artery disease.
Automatic Algorithm of Magnetic Resonance Morphometry in the Diagnosis of Focal Cortical Dysplasia
Authors: A. M. Shevchenko, E. L. Pogosbekyan, A. I. Batalov, E. I. Shultz, A. N. Tyurina, L. M. Fadeeva, M. V. Shevchenko, P. A. Vlasov, N. E. Zakharova, A. G. Melikyan, I. N Pronin
Journal: Radiology - Practice (2021)

The purpose of the study — to create an original algorithm of MR-morphometry for identifying FCD zones. Based on the use of the ANTs and FSL programs, an algorithm for MR morphometry was developed. It was used to generate maps of the z-index of the blur of the transition of gray and white matter and the thickness of the crust (Junction and thickness maps). An algorithm for automatic detection of focal cortical dysplasia zones has been developed. The MRI morphometry method is a promising technique for additional assessment of pathological changes in focal cortical dysplasia.
Venous Angioma of the Deep Brain Structures which Caused Aqueductal Stenosis (Сlinical Case and the Review of the Literature)
Authors: K. D. Solozhentseva, K. V. Shevchenko, N. E. Zakharova, R. M. Afandiev, I. N. Pronin
Journal: Radiology - Practice (2021)

Venous angioma is a common developmental abnormality of the cerebral venous vessels. Usually, this pathology is not accompanied by any symptoms and is detected accidentally during MRI studies conducted for other reasons. In our article, we present a rare clinical case when venous angioma caused symptoms due to its location, which led to the development of the aqueductal stenosis.
Acute Neurological Brain Lesions in the Structure of the Clinical Picture of Severe Respiratory Syndrome-2 (SARS-CoV-2)
Authors: G. N. Dorovskikh, S. S. Sedelnikov, S. A. Kozhedub, D. A. Sulim, Ya. A. Biler, A. A. Podenkova
Journal: Radiology - Practice (2021)

The coronavirus of severe acute respiratory syndrome-2 (SARS-CoV-2) and the coronavirus infection caused by it, along with damage to the respiratory system, can lead to disorders of the central and peripheral nervous system. The article presents literature data and own observations of neurological disorders in patients with coronavirus disease in the superacute period. Pronounced neurological disorders are mainly observed in severe coronavirus disease and include acute ischemic stroke, subcortical microand macro— bleeding, acute necrotizing encephalopathy, Guillain-Barre syndrome. Factors potentially complicating the course of coronavirus disease and contributing to the development of neurological complications are hypertension, diabetes mellitus, chronic diseases of the heart and respiratory system. Based on existing publications and our own observations, we have systematized information about the relationship between coronavirus disease and neurological disorders, about possible factors contributing to the occurrence of stroke. The possibilities of using chest MSCT as the only method of radiation diagnostics for the early detection of viral pneumonia in patients receiving emergency and emergency care at the regional vascular center of BUZOO «GC BSMP No. 1» are considered. The use of this method, in the ultra-acute period of acute ischemic stroke, during the first 40 minutes from the moment of admission, can significantly reduce the time of diagnosis and mortality in patients with acute ischemic stroke and viral pneumonia.
Diagnosis of Spinal Metameric Arteriovenous Malformation Syndrome (Case Report)
Authors: E. V. Rasulova, I. N. Pronin, R. M. Afandiev, N. E. Zakharova
Journal: Radiology - Practice (2021)

This paper presents a rare case of spinal arteriovenous metameric syndrome (SAVMS) with lesions localized in the lower thoracic spine with the presence of two closely spaced arteriovenous malformations intramedullary and extradural (intravertebral) localization. The possibilities of minimally invasive contrast dynamic MR-angiography (TRICKS) in the diagnosis of the main components of vascular malformations and in determining the level of their arterial blood supply have been demonstrated.
Experience of Application of Magnetic Resonance Myelography Methods in Patients with Neurological and Neurosurgical Pathology
Authors: O. B. Bogomyakova, L. M. Vasilkiv, Yu. A. Stankevich, A. A. Savelov, A. A. Tulupov
Journal: Radiology - Practice (2021)

Purpose — to study the possibilities of clinical application of MR-myelography techniques in static and dynamic modes in patients with various neurological and neurosurgical pathologies accompanied by CSF dynamics disorders. On the Philips 1.5 and 3.0 T MRI scanners, an extended protocol for examination of patients with cerebrospinal fluid disorders was presented. The protocol includes, in addition to routine sequences (T1-, T2-WI, FLAIR), thin-slice MP-myelography in a static mode (CSF-DRIVE, 3DMYUR) with soft tissues signal suppression. Also, a cinema-technique of MR myelography (CSF-PCA) in the optimal projection and a phase-contrast method with a quantitative assessment of the cerebrospinal fluid flow at the pathology level and at adjacent levels were performed. The assessment of the diagnostic significance of the proposed research protocol was carried out. The extended study protocol was applied in patients with idiopathic normotensive hydrocephalus, obstructive hydrocephalus, Chiari malformation type I, in patients with syringomyelia at the level of the cervical spinal cord and showed high efficiency in assessing of cerebrospinal fluid dynamics in patients with a neurological and neurosurgical profile. The use of extended approach allows to more fully evaluate the state of the cerebrospinal fluid system, the patency of the cerebrospinal fluid (the presence of additional septa / membranes, partial / complete stenosis and narrowing), as well as preand postoperative morpho-functional changes.
Diffusion-tensor and Diffusion-kurtosis Magnetic Resonance Imaging in the Assessment of Diffuse Axonal Injury (Literature Review)
Authors: R. M Afandiev, N. E. Zakharova, E. L. Pogosbekyan, A. A. Potapov, I. N. Pronin
Journal: Radiology - Practice (2021)

Traumatic brain injury (TBI) is the main cause of disability and mortality among young people, and neurological dysfunctions can persist many years after injury. One of the main type of TBI is diffuse axonal injury (DAI). The prevalence of diffuse axonal injury is often underestimated in computed tomography (CT) and routine magnetic resonance imaging (MRI). In such cases, diffusion-tensor MRI (DT MRI) and diffusion-kurtosis MRI (DK MRI) provide additional information about the integrity of the brain tissue, which cannot be obtained using standard MRI. In this review, we examined the contribution of DT MRI and DK MRI methods to understanding the pathophysiology and prognosis of DAI to help experts interested in planning new studies and participating in the care of patients with TBI.
Abstract 13184: ATP Citrate Lyase a New Therapeutic Target for Vascular Remodeling Diseases
Authors: Yann Grobs, Sarah-eve Lemay, Charlotte Romanet, Sandra Breuils Bonnet, Mark Orcholski, Alice Bourgeois, Melanie Sauvaget, Kana Shimauchi, Pierre Voisine, Roxane Paulin, Francois Potus, Steeve Provencher, Olivier BOUCHERAT, Sebastien Bonnet
Journal: Circulation (2021)

Vascular remodeling diseases (VRD) like coronary artery diseases or in-stent restenosis are characterized by proliferative and apoptosis-resistant vascular smooth muscle cells (VSMCs). As in cancer, proliferative VSMCs have increased Warburg effect leading to an impairment in the acetyl-coA homeostasis resulting in a profound epigenetic reprogramming sustaining their survival overtime. ATP citrate lyase (ACLY) is a key enzyme of the acetyl-coA and cholesterol metabolism involved in cell survival and resistance to apoptosis, in part by promoting the Warburg effect and histone acetylation. Surprisingly, its role in VRD remains unknown. We hypothesized that ACLY inhibition reverses VRD. Using immunofluorescence and western blot, we showed a significant (p&lt;0.05) upregulation of ACLY in remodeled distal coronary arteries (n=5), stenosed saphenous vein (n=3) and remodeled radial arteries (n=5) isolated from patients with VRD compared to non VRD donors. In vitro , we found that PDGF/FGF2-stimulated human rings of mammary arteries, saphenous veins or cultured VSMCs isolated from coronary arteries exhibit increased expression as well as AKT-dependent activation and nuclear accumulation of ACLY (p&lt;0.05). These effects were associated with a profound increase in cell proliferation (Ki67) and repression of apoptosis (Annexin V). Consistently, acetylation-dependent upregulation of the pro-proliferative markers PCNA, MCM2 and PLK1 (p&lt;0.05) and the cell survival marker SURVIVIN was observed (p&lt;0.05)). All these effects were dose-dependently inhibited by pharmacological inhibition of ACLY using BMS303141. Using a standard in vivo rat carotid injury model, we showed that the clinically available ACLY inhibitor ETC-1002 (n=10) prevents carotid remodeling 14 days post-injury compared to vehicle treated rats (n=10) (p&lt;0.05). We demonstrated that ACLY upregulation in VRD is associated with vascular remodeling through increased smooth muscle cell proliferation and resistance to apoptosis. In vivo , ETC-1002 prevents remodeling in a rat carotid injury model. In vivo experiments using Acly smooth muscle cell-specific knockout mice are currently performed to further characterize the therapeutic potential of ACLY inhibition in VRD.
Endoscopy International Open – recently published
Journal: Endoscopy (2021)

Comment on: Safety and effectiveness of endoluminal vacuum-assisted closure for esophageal defects: Systematic review and meta-analysisEndosc Int Open 2021; 09(09): E1371-E1380DOI: 10.1055/a-1508-5947 Comment on: Double tunnel technique reduces technical failure during POEM in cases with severe submucosal fibrosisEndosc Int Open 2021; 09(09): E1335-E1341DOI: 10.1055/a-1499-6817 Comment on: A deep learning framework for autonomous detection and classification of Crohnʼs disease lesions in the small bowel and colon with capsule endoscopyEndosc Int Open 2021; 09(09): E1361-E1370DOI: 10.1055/a-1507-4980
Endoscopy International Open – recently published
Journal: Endoscopy (2021)

Comment on: Short versus standard esophageal myotomy in achalasia patients: a systematic review and meta-analysis of comparative studiesEndosc Int Open 2021; 09(08): E1246-E1254DOI: 10.1055/a-1490-8493 Comment on: Long-term outcome after EUS-guided radiofrequency ablation: Prospective results in pancreatic neuroendocrine tumors and pancreatic cystic neoplasmsEndosc Int Open 2021; 09(08): E1178-E1185DOI: 10.1055/a-1479-2199 Comment on: Video clips compared with high-definition still images for characterization of colorectal neoplastic lesions: a randomized comparative prospective studyEndosc Int Open 2021; 09(08): E1255-E1263DOI: 10.1055/a-1487-5628
Cognitive-motor dissociation and time to functional recovery in patients with acute brain injury in the USA: a prospective observational cohort study
Authors: Jennifer Egbebike, Qi Shen, Kevin Doyle, Caroline A Der-Nigoghossian, Lucy Panicker, Ian Jerome Gonzales, Lauren Grobois, Jerina C Carmona, Athina Vrosgou, Arshneil Kaur, Amelia Boehme, Angela Velazquez, Benjamin Rohaut, David Roh, Sachin Agarwal, Soojin Park, E Sander Connolly, Jan Claassen
Journal: The Lancet Neurology (2022)
Interferon receptor dysfunction in a child with malignant atrophic papulosis and CNS involvement
Authors: Lena-Luise Becker, Frédéric Ebstein, Denise Horn, Christos C Zouboulis, Elke Krüger, Angela M Kaindl, Anna Tietze, Angelika Eger, Tilmann Kallinich, Saskia Biskup, Simone Schmid, Werner Stenzel, Hanna Münzfeld, Ulrike Blume-Peytavi
Journal: The Lancet Neurology (2022)
Efficacy and safety of neoadjuvant immunotherapy in surgically resectable esophageal cancer: A systematic review and meta-analysis
Authors: Zhaoyang Wang, Changjian Shao, Yuanyong Wang, Hongtao Duan, Minghong Pan, Jinbo Zhao, Jian Wang, Zhiqiang Ma, Xiaofei Li, Xiaolong Yan
Journal: International Journal of Surgery (2022)
The Boston criteria version 2.0 for cerebral amyloid angiopathy: a multicentre, retrospective, MRI–neuropathology diagnostic accuracy study
Authors: Andreas Charidimou, Gregoire Boulouis, Matthew P Frosch, Jean-Claude Baron, Marco Pasi, Jean Francois Albucher, Gargi Banerjee, Carmen Barbato, Fabrice Bonneville, Sebastian Brandner, Lionel Calviere, François Caparros, Barbara Casolla, Charlotte Cordonnier, Marie-Bernadette Delisle, Vincent Deramecourt, Martin Dichgans, Elif Gokcal, Jochen Herms, Mar Hernandez-Guillamon, Hans Rolf Jäger, Zane Jaunmuktane, Jennifer Linn, Sergi Martinez-Ramirez, Elena Martínez-Sáez, Christian Mawrin, Joan Montaner, Solene Moulin, Jean-Marc Olivot, Fabrizio Piazza, Laurent Puy, Nicolas Raposo, Mark A Rodrigues, Sigrun Roeber, Jose Rafael Romero, Neshika Samarasekera, Julie A Schneider, Stefanie Schreiber, Frank Schreiber, Corentin Schwall, Colin Smith, Levente Szalardy, Pascale Varlet, Alain Viguier, Joanna M Wardlaw, Andrew Warren, Frank A Wollenweber, Marialuisa Zedde, Mark A van Buchem, M Edip Gurol, Anand Viswanathan, Rustam Al-Shahi Salman, Eric E Smith, David J Werring, Steven M Greenberg
Journal: The Lancet Neurology (2022)
Safety and efficacy of rituximab versus dimethyl fumarate in patients with relapsing-remitting multiple sclerosis or clinically isolated syndrome in Sweden: a rater-blinded, phase 3, randomised controlled trial
Authors: Anders Svenningsson, Thomas Frisell, Joachim Burman, Jonatan Salzer, Katharina Fink, Susanna Hallberg, Joakim Hambraeus, Markus Axelsson, Faiez Al Nimer, Peter Sundström, Martin Gunnarsson, Rune Johansson, Johan Mellergård, Igal Rosenstein, Ahmad Ayad, Irina Sjöblom, Anette Risedal, Pierre de Flon, Eric Gilland, Jonas Lindeberg, Fadi Shawket, Fredrik Piehl, Jan Lycke
Journal: The Lancet Neurology (2022)
Numb and Numblike regulate sarcomere assembly and maintenance
Authors: Baolei Wang, Min Yang, Shujuan Li
Journal: Journal of Clinical Investigation (2022)

The description of Figure 3E in the Results section has also been corrected, as below:The results indicate that Numb (173-322 aa) and Numblike (1-366 aa) were the dominant residue sequences that interacted with ACTC1 and that Numblike (1-205 aa) and Numblike (367-604 aa) had negligible functional interactions with ACTC1 (Figure 3F).After blasting the Numb (173-322 aa) and Numblike (1-366 aa) amino acid sequences in the NCBI database, we found that these 2 sequences were highly conserved, indicating the potentially critical role of these conservative domains in α-Actin binding (Supplemental Figure 9A).In the Discussion section, a corrected sentence has been included, as below:Since the expression of MLC-2v is restricted within the ventricular chamber throughout mouse embryonic development, this mouse line serves as a great platform to study how Numb and Numblike affect sarcomere assembly in ventricular CMs at the early stage (19, 26).The text and Figure 3E have been updated in the HTML version and PDF with the correct information.
Inhibiting DNA methylation improves antitumor immunity in ovarian cancer
Authors: Katherine B. Chiappinelli, Stephen B. Baylin
Journal: Journal of Clinical Investigation (2022)

Cancer cells resist the immune response in a process known as immune editing or immune evasion. Therapies that target the immune system have revolutionized cancer treatment; however, immunotherapies have been ineffective for the majority of ovarian cancer cases. In this issue of the JCI, Chen, Xie, et al. hypothesized that hypomethylating agent (HMA) treatment would induce antitumor immunity to sensitize patients with ovarian cancer to anti-PD-1 immunotherapy. The authors performed a phase II clinical trial to test the combination of guadecitabine, a second-generation HMA, along with pembrolizumab, an immune checkpoint inhibitor of PD-1. The trial included a group of 35 patients with platinum-resistant ovarian cancer. While the clinical benefit from the combined HMA plus immune checkpoint blockade regimen was lower than hoped, the correlate analyses gave important information about which patients with ovarian cancer may be more likely to respond to immune therapy.
CD11b suppresses TLR activation of nonclassical monocytes to reduce primary graft dysfunction after lung transplantation
Authors: Melissa Querrey, Stephen Chiu, Emilia Lecuona, Qiang Wu, Haiying Sun, Megan Anderson, Megan Kelly, Sowmya Ravi, Alexander V. Misharin, Daniel Kreisel, Ankit Bharat, G.R. Scott Budinger
Journal: Journal of Clinical Investigation (2022)

Primary graft dysfunction (PGD) is the leading cause of postoperative mortality in lung transplant recipients and the most important risk factor for development of chronic lung allograft dysfunction. The mechanistic basis for the variability in the incidence and severity of PGD between lung transplant recipients is not known. Using a murine orthotopic vascularized lung transplant model, we found that redundant activation of Toll-like receptors 2 and 4 (TLR2 and -4) on nonclassical monocytes activates MyD88, inducing the release of the neutrophil attractant chemokine CXCL2. Deletion of Itgam (encodes CD11b) in nonclassical monocytes enhanced their production of CXCL2 and worsened PGD, while a CD11b agonist, leukadherin-1, administered only to the donor lung prior to lung transplantation, abrogated CXCL2 production and PGD. The damage-associated molecular pattern molecule HMGB1 was increased in peripheral blood samples from patients undergoing lung transplantation after reperfusion and induced CXCL2 production in nonclassical monocytes via TLR4/MyD88. An inhibitor of HMGB1 administered to the donor and recipient prior to lung transplantation attenuated PGD. Our findings suggest that CD11b acts as a molecular brake to prevent neutrophil recruitment by nonclassical monocytes following lung transplantation, revealing an attractive therapeutic target in the donor lung to prevent PGD in lung transplant recipients.