tbuckley/cabot-search huggingface dataset:
TCTAP C-051 No Time for Surgeon an Emergency Critical Left Main Primary PCI Using IVUS
Authors: Vijayendran Rajalingam, Dharmaraj Karthikesan, Saravanan Krishinan, Kantha Rao Narasamuloo, Liew Kai Soon, Yuen Hoong Phang, Chee Tat Liew, Tamim Ansari Bin Jahubar Sathik, Pirevina Naidu Krishnan Naidu, Nur Asmalina Binti Azizan, Izzatul Nadzirah Binti Ismail, Ahmad Faiz Mohd Ezanee
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-086 Can a Machine Learning Based Approach Improve Risk Prediction and Individualise Decision Making Between Percutaneous and Surgical Revascularisation in Patients With Complex Coronary Artery Disease?
Authors: Kai Ninomiya, Shigetaka Kageyama, Hiroki Shiomi, Shinichiro Masuda, Nozomi Kotoku, Pruthvi Revaiah, Scot Garg, Neil O’Leary, Takeshi Kimura, Yoshinobu Onuma, Patrick W. Serruys
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-152 Successful Treatment of Subclavian Artery Thrombosis by Catheter-Based Thrombus Aspiration and Thrombolytic Therapy: A Case Report
Authors: A.P.M. Sohrabuzzaman, Shiblee Sadik Pathan, Md Mohasin Uddin, Mohammad Abul Khayer, Rashiduz Zaman, Asifudduza Asif, Rakibul Hasan, Fahmida Zaman
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-054 “CROSSVAC” - A New Flossing Strategy for Severe Calcified Lesion
Authors: Yukihiro Yamaguchi, Hideyuki Takimura, Reiko Tsukahara, Masatsugu Nakano, Satoru Nishio, Yukako Takimura, Mami Kawano, Emi Tajima, Ippei Tsuzuki, Rintaro Taniguchi
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-035 Coronary Lithotripsy for Underexpanded Stent and Orbital Atherectomy for Severely Calcified Native Coronary in NSTE-ACS
Authors: Mohd Tawfeq Mohd Noor, Balachandran Kandasamy, Khai Chih Teh, Faten Aqilah Aris, Jassie Teo
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-103 A Case Series of Snare-Assisted Transcatheter Aortic Valve Implantation in Challenging Anatomy
Authors: Kar Lok Leo Lai, Kevin Ka-Ho Kam, Ka Lung Chui, Randolph Hung-Leung Wong, Alex Pui Wai Lee, Eugene Brian Wu, Kent Chak Yu So
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-026 The Comparison of Hemostatic Strategies After Percutaneous Coronary Intervention: A Systematic Review and Meta-Analysis
Authors: Anastasya Maria Kosasih, Gusti Ngurah Prana Jagannatha, I Wayan Agus Surya Pradnyana, Putu Febry Krisna Pertiwi, Ida Bagus Satriya Wibawa, Luh Oliva Saraswati Suastika
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-068 Use of FFR in Chronic Stable Angina Patients and Its Impact on PCI and In-Hospital Outcome
Authors: Smita Kanungo, Fazila Malik, Nazir Ahmed, Mohammad Badiuzzaman, Ashok Kumar Dutta, Dhiman Banik, Md Kalimuddin, Nazmun Laila, Shamim Chowdhury
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-118 Iatrogenic Aorto-Coronary Dissection of the Right Coronary Artery During Diagnostic Angiography Using a Tiger Catheter
Authors: Ronald Santos, Meliton III Aquines Evangelista, Alwyn Susanto, Conrad Estanislao, Paterno Frias Dizon, Joanne Cosare San Pedro
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-037 Orbitotripsy in Emergent PCI: The Combined Use of Orbital Atherectomy and Intravascular Lithrotripsy for Heavily Calcified Coronary Lesions in a Patient Presented With Acute ST-Segment Elevation Myocardial Infarction
Authors: Siu Fung Wong, Chun Yue Mui, Tak-Shun Chung
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-082 Novel CHA2DS2-VASc-HSF as a Predictor In-Stent Restenosis in Coronary Artery Disease Patient After Stent Implantation
Authors: Ronaldi Rizkiawan, Ivana Purnama Dewi, Louisa Fadjri Kusuma Wardhani, I Gde Rurus Suryawan, Andrianto Andrianto
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-016 Immediate Versus Deferred Stenting for ST Elevation Myocardial Infarct With High Burden Thrombus: A Meta-Analysis From Different Perspective
Authors: Hendy Bhaskara Perdana Putra, Noer Halimatus Syakdiyah, Yusra Pintaningrum, Maria Adventia Debi Mustika Martin
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-055 Let’s Save the Heart: A Complex Coronary Intervention in a Patient With Distal Left Main Stenosis and Heart Failure With Reduced Ejection Fraction
Authors: Kumutha Gobal, Muhamad Ali SK Abdul Kader, Adelyn Nisha Henry, Vicknesan Kulasingham, Gurudevan Mahadevan, Tee Choon Keong
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-181 Pulmonary Valve Balloon Dilation and Transcatheter Closure of Atrial Septal Defect in a Teenaged Cyanotic Girl
Authors: Abdul Momen, Ashraf Ur Rahman, Lima Asrin Sayami, ABM Abm Nurunnobi, Md Saqif Shahriar, Md Zulfikar Ali
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-055 A Comparison of Pharmaco-Mechanical Thrombolysis and Catheter-Directed Thrombosis for Treatment of Thromboembolic Occlusion of Lower Limb – An Observational Case-Control Study.
Authors: Chun-Cheng Wang, Chung-Ho Hsu, Chiung-Ray Lu, Yukai Lin
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-037 Feasibility and Accuracy of Holographic Graft Length Measurement, A Sub-study of the FAST TRACK CABG Trial
Authors: Tsung Ying Tsai, Shigetaka Kageyama, Yoshinobu Onuma, Giulio Pompilio, Daniele Andreini, Saima Mushtaq Saima Mushtaq, Mark La Meir, Johan De Mey, Kaoru Tanaka, Torsten Doenst, John D. Puskas, Patrick W. Serruys
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-071 Comparison of Perindopril vs. Losartan With Regard to Pro-Coagulation Factors in Adipocytes Exposed to Sars-Cov-2 Spike Protein
Authors: Hanestya Oky Hermawan, Primasitha Maharany Harsoyo, Makhyan Jibril Al-Farabi, I Gde Rurus Suryawan, Meity Ardiana
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-014 Pharmacoinvasive Therapy in Treating Acute STEMI Patients in COVID-19 Era-Thrombolysis by Tenecteplase (TNK) / Streptokinase (STK) Followed by Rescue PCI of the Culprit Vessel
Authors: Ahm Waliul Islam, A.Q.M. Reza, Shams Munwar, Shahab Uddin Talukder, Tamzeed Ahmed Tamzeed Ahmed, Kazi Atiqur Rahman, Azfar Hossain Bhuiyan
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-044 Early Mechanical Circulatory Support in Myocardial Infarction Complicated by Cardiogenic Shock: A Nationwide Experience in Comparison to International Experience
Authors: Khaled Al Khodari, Alaa Rahhal, Raad Tahtouh, Mohammed Awad Ashour, Eman Shehada, Wael Kanjo, Mohamad Safwan Aljafar, Mona Sofi, Abdelaziz M. Tawengi, Mohamed Mahmoud Tawengi, Abdul Rahman Arabi
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-132 Optical Coherence Tomography Guided Circumflex Artery Stenting Complicated by Left Main Occlusion Treated by Intravascular Ultrasound Guided Left Main and Left Descending Artery Stenting
Authors: Farhat Fouladvand, Mauro Musmeci, Guido Belli
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-120 A Challenging Complication of Percutaneous Coronary Intervention – Stent Edge Dissection and Intimal Hematoma Formation
Authors: Withanage Narmada Madhubhashini Perera, Gamini Galappaththy, Chamidi Isanka Hasani Siriwardane, Naigalage Upananda Ajith Dissanayake
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-013 Association of Cardiovascular Events and Long-Term Exposure to Fine Particulate Matter Among Survivors of ST-Elevation Myocardial Infarction Who Received Percutaneous Coronary Intervention
Authors: Teeranan Angkananard, Phannita Piriyavirut, Phattarawadee Mathupayas, Naravit Kowathanakul, Bulakorn Thawornrunggit, Thiris Laohapornsvan, Arthit Vongsaosup, Nattapun Rattanajaruskul, Orrawan Wansawad, Worawut Roongsangmanoon
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-046 A 30-Day Pooled Analysis of Acetyl Salicylic Elimination Trials (ASET) In Brazil and Japan: Synergy Stent With Prasugrel Monotherapy Without Aspirin
Authors: Yuki Ishibashi, Shinichiro Masuda, Takashi Muramatsu, Ken Kozuma, Hideyuki Kawashima, Gaku Nakazawa, Kuniaki Takahashi, Kengo Tanabe, Norihiro Kogame, Masato Nakamura, Taku Asano, Takayuki Okamura, Yosuke Miyazaki, Hiroki Tateishi, Yukio Ozaki, Shimpei Nakatani, Yuki Katagiri, Yoshihiro Morino, Kai Ninomiya, Shigetaka Kageyama, Nozomi Kotoku, Patricia O. Guimarães, Pedro Lemos, Yoshinobu Onuma, Patrick W. Serruys
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-069 Evaluation of Instantaneous Wave-Free Ratio and Fractional Flow Reserve in Severe Aortic Valve Stenosis
Authors: Ha Hye Jo, Yeonwoo Choi, Hoyun Kim, Jinho Lee, Do-Yoon Kang, Jung-Min Ahn, Duk-Woo Park, Seung-Jung Park
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-090 Investigation of Strategies to Reduce Contrast Media Usage and Risk of Contrast Induced Renal Injury During PCI: A “Low Contrast Volume Protocol” Introduction to a Daily Clinical Practice
Authors: Aiste Zebrauskaite, Greta Ziubryte, Lukas Mackus, Austeja Lieponyte, Evelina Kairyte, Gediminas Jarusevicius
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-043 Precise Assessments of Dissection Following Drug-Coated Balloon Treatment and Its Impact on Clinical Outcomes in de Novo Small Vessel Disease
Authors: Kai Ninomiya, Patrick W. Serruys, Jiayue Huang, Shinichiro Masuda, Daixin Ding, Nozomi Kotoku, Shigetaka Kageyama, Shengxian Tu, William Wijns, Jouke Dijkstra, Sandeep Basavarajaiah, Antonio Colombo, Yoshinobu Onuma
Journal: Journal of the American College of Cardiology (2023)
Systematic elucidation of genetic mechanisms underlying cholesterol uptake
Authors: Marisa C. Hamilton, James D. Fife, Ersin Akinci, Tian Yu, Benyapa Khowpinitchai, Minsun Cha, Sammy Barkal, Thi Tun Thi, Grace H.T. Yeo, Juan Pablo Ramos Barroso, Matthew Jake Francoeur, Minja Velimirovic, David K. Gifford, Guillaume Lettre, Haojie Yu, Christopher A. Cassa, Richard I. Sherwood
Journal: Cell Genomics (2023)

Genetic variation contributes greatly to LDL cholesterol (LDL-C) levels and coronary artery disease risk. By combining analysis of rare coding variants from the UK Biobank and genome-scale CRISPR-Cas9 knockout and activation screening, we substantially improve the identification of genes whose disruption alters serum LDL-C levels. We identify 21 genes in which rare coding variants significantly alter LDL-C levels at least partially through altered LDL-C uptake. We use co-essentiality-based gene module analysis to show that dysfunction of the RAB10 vesicle transport pathway leads to hypercholesterolemia in humans and mice by impairing surface LDL receptor levels. Further, we demonstrate that loss of function of
TCTAP C-199 Left Main Coronary Artery Thrombosis During Transcatheter Aortic Valve Implantation
Authors: Mary Jane Penelope Diaz Gatpatan, Alwyn Susanto, Terence Cuezon, Douglas Bailon, Ferdinand Alzate, Fabio Enrique Posas
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-201 Optimal Implantation Depth of a Self-Expandable TAVR Device in a Patient With Type 0 Bicuspid Aortic Valve With Severe Stenosis and Calcification Guided by In-Silico Simulation
Authors: Xinlei Wu, Patrick W. Serruys, Xiaojie Yu, Yuanxue Sun, Yanru Chen, Daozhu Wu, Xinmin Zhang, Lianpin Wu
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-198 All Roads Lead to Rome
Authors: Kar Lok Leo Lai, Kevin Ka-Ho Kam, Sylvia S.W. Au, Ka Lung Chui, Randolph Hung-Leung Wong, Alex Pui Wai Lee, Bryan Ping-Yen Yan, Eugene Brian Wu, Joseph Y.S. Chan, Kent Chak Yu So
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-149 A Case Report of Class II Endovenous Heat-Induced Thrombosis Post Endovenous Laser Ablation: Prompt Treatment or Watchful Waiting?
Authors: Ifan Citra, Fery Budiman Santoso, Vekky Sariowan, Janry Antonius Pangemanan, Agnes Lucia Panda
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-019 Primary Percutaneous Coronary Intervention in a Patient With Single Coronary Artery Ostium Experiencing ST-Elevation Myocardial Infarction
Authors: Ricardo Adrian Nugraha, Bagus Khrisna, Tony Santoso Putra, Lalu Galih Pratama Rinjani, I Gde Rurus Suryawan, Yudi Her Oktaviono
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-176 Percutaneous Coronary Intervention to Left Main and Left Anterior Descending Artery With Rotational Atherectomy via Left Distal Radial Access
Authors: Meei Wah Chan, Benjamin Tao Xiung Lim, Abdul Raqib Abd Ghani, Kamaraj Selvaraj
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-200 An Interesting Complex Coronary / Valvular / Electrophysiological Intervention
Authors: Nithesh Kumar, Thoddi Ramamurthy Muralidharan, S. Sadhanandham, Nagendra Boopathy Senguttuvan, Manokar Panchanatham, Ramesh Sankaran, Vinod Kumar Balakrishnan
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-190 Successful Left Ventricular Unloading With Atrial Septal Stenting During Extracorporeal Membrane Oxygenation as a “Bridge to Heart Transplantation” in Patient With Severe Mitral Paravalvular Leakage
Authors: Thitima Limjaroen, Supanee Sinphurmsukskul, Vorarit Lertsuwunseri, Suphot Srimahachota
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-197 Difficult Transcatheter Aortic Valve Implantation in a Bicuspid Aortic Stenosis With Eccentric Calcification and a Horizontal Ascending Aorta
Authors: Debdatta Bhattacharyya, Manik Chopra, Ayan Kar, Snehil Goswami
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-187 Transcatheter Closure of Coronary Cameral Fistula From Right Coronary Artery to Right Atrium With Cocoon Patent Ductus Arteriosus Device
Authors: Polavith Chutisira, Vorarit Lertsuwunseri, Siriporn Athisakul, Suchat Sricholwattana, Suphot Srimahachota
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-167 QFR Guided PCI the New Modality
Authors: Sabapathy Diagarajan, Gurpreet Pal Singh Jugindar Singh, Mohd Ruslan Mustapa, Kengeswari Raja, Chen Tai Meng, Yew Fung Kwan, Hazleena Mohamed Hasnan, Nor Hanim Mohd Amin
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-172 Triglyceride Deposit Cardiomyovasculopathy, a Novel Heart Disease Refractory to Conventional Treatment in Patients With Cardiac Ischemia and Cardiac Dysfunction Diagnosed With BMIPP Scintigraphy
Authors: Atomu Tajima, Yusuke Nakano, Akihiro Suzuki, Kento Kikuchi, Masanobu Fujimoto, Hirohiko Ando, Tetsuya Amano
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-186 A Challenging Case Report: Transapical Vascular Plug to Occlude Large Pseudoaneurysm of the Mitro-Aortic Intervalvular Fibrosa (P-MAIVF) In Recurrent Prosthetic Valve Endocarditis
Authors: Panupong Pota, Tasalak Thonghong, Noppon Taksaudom
Journal: Journal of the American College of Cardiology (2023)
TCTAP A-035 Fractional Flow Reserve in Left Main Bifurcation From a Single Fluoroscopic View Versus Computed Tomography
Authors: Nozomi Kotoku, Daixin Ding, Kai Ninomiya, Neil O’Leary, William Wijns, Shengxian Tu, Nicolo Piazza, Yoshinobu Onuma, Patrick W. Serruys
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-203 An Inoperable Non-calcified Severe Aortic Regurgitation Case Treated by Balloon Expandable Transcatheter Aortic Valve Replacement
Authors: Thinnakrit Sasiprapha, Mann Chandavimol, Pavit Pienvichit, Tawai Ngernsritrakul
Journal: Journal of the American College of Cardiology (2023)
TCTAP C-196 Massive Pulmonary Embolism Mimicking Acute Coronary Syndrome in a Young Man
Authors: Mohd Asyiq Al-Fard Bin Mohd Raffali, Boon Cong Beh, Diyana Farouk, Shawal Faizal Mohamad, Hamat Hamdi Che Hassan
Journal: Journal of the American College of Cardiology (2023)
Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair
Authors: Tianchang Wang, Wentao Li, Yuxin Zhang, Xiang Xu, Lei Qiang, Weiqiang Miao, Xiaokun Yue, Xin Jiao, Xianhao Zhou, Zhenjiang Ma, Shuai Li, Muliang Ding, Junfeng Zhu, Chi Yang, Hui Wang, Tao Li, Xin Sun, Jinwu Wang
Journal: Bioactive Materials (2023)

Crosstalk between nerves and bone is essential for bone repair, for which Schwann cells (SCs) are crucial in the regulation of the microenvironment. Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair, the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes (SC-exos) on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve–bone crosstalk. SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) through exosomes. Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-β signaling pathway via let-7c-5p. Interestingly, SC-exos promoted the migration and tube formation performance of endothelial progenitor cells. Furthermore, the [email protected]/S constructs were developed by bioprinting technology that simulated SC-mediated nerve–bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos, exerting the regulatory effect of SCs in the microenvironment to promote innervation, vascularization, and osteogenesis and thus effectively improving bone repair in a cranial defect model. This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair.
Adipose-derived stem cell/FGF19-loaded microfluidic hydrogel microspheres for synergistic restoration of critical ischemic limb
Authors: Ruihan Wang, Fangqian Wang, Shan Lu, Bin Gao, Yuanqing Kan, Tong Yuan, Yisheng Xu, Chen Yuan, Daqiao Guo, Weiguo Fu, Xiaohua Yu, Yi Si
Journal: Bioactive Materials (2023)

The efficacy of stem cell therapy is substantially compromised due to low cell survival rate and poor local retention post-delivery. These issues drastically limit the application of stem cells for ischemic limb therapy, which requires effective blood perfusion and skeletal muscle regeneration. Herein, based on microfluidic technology, an integrated stem cell and cytokine co-delivery system designed for functional ischemic limb salvage was constructed by first incorporating the myogenic cytokine, fibroblast growth factor 19 (FGF19), into microspheres composed of methacrylate gelatin (GelMA). Then adipose-derived stem cells (ADSCs) were highly absorbed into the porous structure of the microspheres, overcoming the insufficient loading efficiency and activities by conventional encapsulation strategy. The fabricated ADSCs/FGF19@μsphere system demonstrated a uniform size of about 180 μm and a highly porous structure with pore sizes between 20 and 40 μm. The resultant system allowed high doses of ADSCs to be precisely engrafted in the lesion and to survive, and achieved sustained FGF19 release in the ischemic region to facilitate myoblast recruitment and differentiation and myofibrils growth. Furthermore, the combination of ADSCs and FGF19 exhibited a positive synergistic effect which substantially improved the therapeutic benefit of angiogenesis and myogenesis, both in vitro and in vivo. In summary, a stem cell and cytokine co-delivery system with the properties of easy preparation and minimal invasiveness was designed to ensure highly efficient cell delivery, sustained cytokine release, and ultimately realizes effective treatment of ischemic limb regeneration.
Bacteriuria of Pregnancy
Authors: Marvin Turck, Bernard S. Goffe, Robert G. Petersdorf
Journal: New England Journal of Medicine (1962)

ALTHOUGH symptomatic urinary-tract infections are diagnosed frequently during pregnancy, recent reports indicate that a number of pregnant women have bacteriuria that remains asymptomatic and escapes clinical recognition unless quantitative urine cultures are obtained. For example, Kass1 2 3 has shown that 6 to 7 per cent of 4000 pregnant women attending the prenatal clinics of the Boston City Hospital had asymptomatic bacteriuria. Henderson and his co-workers4 found the incidence of unrecognized urinary-tract infections to be 6.5 per cent in pregnant Negro women whereas only 2.5 per cent of white women, presumably of a similar low socioeconomic status, had cryptic bacteriuria. Kaitz . . .
Abstract LB250: Multiplex screening of PBMCs with DNA barcoded peptide exchanged MHC trimers to identify peptide-specific paired VDJ TCRs at single cell level
Authors: Marc Delcommenne, Xiaohong Hou, Yuri Poluektov, Wenjing Pan, Emily Sims, Miranda Byrne-Steele
Journal: Cancer Research (2023)

Abstract Elucidation of the paired TCR receptor-antigen synapse is important both for vaccine development and for targeted therapy. Linkage of T cell responses with specific antigenic peptides will not only guide the development of immunotherapies but also enable treatment response monitoring. Currently, single cell sequencing technology is well established, but the identification of specific pathogenic peptides and associated TCR in single cell level is still challenging. In most cases, ascertaining T cell responses to pathogenic peptides is based on the screening of pooled peptides that do not give the knowledge of corresponding responsive TCR to a particular peptide. We therefore developed a technique that uses DNA barcoded peptide exchanged MHC trimers to stain PBMCs. With this technique, multiple peptide-loaded trimers can be used to stain a PBMC sample in one test tube. Single cells positive for trimers are sorted, amplified, and sequenced together with the DNA barcode. Specific peptides can be identified with the DNA barcodes which are co-amplified with TCRs. Applying this technique, we screened one PBMC sample with several peptide-loaded trimers in one reaction. Utilizing the CDR3 identity of known clones against each of these peptides, we were able to demonstrate and validate that a multi-peptide screen using DNA barcodes is feasible. Further optimization is in progress to reduce background and to increase the multiplexing of barcoded peptide-trimers. Citation Format: Marc Delcommenne, Xiaohong Hou, Yuri Poluektov, Wenjing Pan, Emily Sims, Miranda Byrne-Steele. Multiplex screening of PBMCs with DNA barcoded peptide exchanged MHC trimers to identify peptide-specific paired VDJ TCRs at single cell level [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 2 (Clinical Trials and Late-Breaking Research); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(8_Suppl):Abstract nr LB250.