tbuckley/cabot-search huggingface dataset:
Ecosystem functioning across the diel cycle in the Anthropocene
Authors: Daniel T.C. Cox, Kevin J. Gaston
Journal: Trends in Ecology & Evolution (2023)

Given the marked differences in environmental conditions and active biota between daytime and nighttime, it is almost inevitable that ecosystem functioning will also differ. However, understanding of these differences has been hampered due to the challenges of conducting research at night. At the same time, many anthropogenic pressures are most forcefully exerted or have greatest effect during either daytime (e.g., high temperatures, disturbance) or nighttime (e.g., artificial lighting, nights warming faster than days). Here, we explore current understanding of diel (daily) variation in five key ecosystem functions and when during the diel cycle they primarily occur [predation (unclear), herbivory (nighttime), pollination (daytime), seed dispersal (unclear), carbon assimilation (daytime)] and how diel asymmetry in anthropogenic pressures impacts these functions.
Reducing the Risks of Nuclear War — The Role of Health Professionals
Authors: Kamran Abbasi, Parveen Ali, Virginia Barbour, Kirsten Bibbins-Domingo, Marcel G M Olde Rikkert, Andy Haines, Ira Helfand, Richard Horton, Bob Mash, Arun Mitra, Carlos Monteiro, Elena N Naumova, Eric J Rubin, Tilman Ruff, Peush Sahni, James Tumwine, Paul Yonga, Chris Zielinski
Journal: New England Journal of Medicine (2023)

our ability to approach each patient with compassion and kindness.
The New USPSTF Mammography Recommendations — A Dissenting View
Authors: Steven Woloshin, Karsten Juhl Jørgensen, Shelley Hwang, H. Gilbert Welch
Journal: New England Journal of Medicine (2023)

Interview with Steven Woloshin on an updated U.S. Preventive Services Task Force recommendation regarding the starting age for mammography screening. (06:33)Download The evidence for breast cancer screening of women in their 40s is insufficient to support a new public health imperative, given the uncertain benefits and common harms for healthy women.
Binding-induced fibrillogenesis peptide inhibits RANKL-mediated osteoclast activation against osteoporosis
Authors: Qi-Lin Liang, Huan-Ge Xu, Long Yu, Meng-Ru Ding, Yu-Ting Li, Gao-Feng Qi, Kuo Zhang, Lei Wang, Hao Wang, Xu Cui
Journal: Biomaterials (2023)
An acid-responsive MOF nanomedicine for augmented anti-tumor immunotherapy via a metal ion interference-mediated pyroptotic pathway
Authors: Zhenzhen Feng, Gui Chen, Min Zhong, Ling Lin, Ziyi Mai, Yan Tang, Guimei Chen, Wen Ma, Guang Li, Yuanyuan Yang, Zhiqiang Yu, Meng Yu
Journal: Biomaterials (2023)
Acute bacterial meningitis due to Neisseria meningitidis serotype B missed by a multiplexed PCR panel
Authors: Mikaël de Lorenzi-Tognon, Vladimir Lazarevic, Nadia Gaïa, Chiraz Chaabane, Abdessalam Cherkaoui, Gesuele Renzi, Jacques Schrenzel
Journal: Clinical Microbiology and Infection (2023)
Hepatocyte TIPE2 is a fasting-induced Raf-1 inactivator that drives hepatic gluconeogenesis to maintain glucose homeostasis
Authors: Yan Tao, Jingyuan Zhao, Jilong Yin, Zixin Zhou, Huijie Li, Jinhao Zang, Tianci Wang, Yalin Wang, Chun Guo, Faliang Zhu, Shen Dai, Fuwu Wang, Hui Zhao, Haiting Mao, Fengming Liu, Lining Zhang, Qun Wang
Journal: Metabolism (2023)
Gut microbiome perturbation, antibiotic resistance, and Escherichia coli strain dynamics associated with international travel: a metagenomic analysis
Authors: Colin J Worby, Sushmita Sridhar, Sarah E Turbett, Margaret V Becker, Lucyna Kogut, Vanessa Sanchez, Ryan A Bronson, Sowmya R Rao, Elizabeth Oliver, Allison Taylor Walker, Maroya Spalding Walters, Paul Kelly, Daniel T Leung, Mark C Knouse, Stefan H F Hagmann, Jason B Harris, Edward T Ryan, Ashlee M Earl, Regina C LaRocque
Journal: The Lancet Microbe (2023)

Culture-based studies have shown that acquisition of extended-spectrum β-lactamase-producing Enterobacterales is common during international travel; however, little is known about the role of the gut microbiome before and during travel, nor about acquisition of other antimicrobial-resistant organisms. We aimed to identify (1) whether the gut microbiome provided colonisation resistance against antimicrobial-resistant organism acquisition, (2) the effect of travel and travel behaviours on the gut microbiome, and (3) the scale and global heterogeneity of antimicrobial-resistant organism acquisition.
Unraveling schwannomas
Authors: Uta E Flucke, Laura S Hiemcke-Jiwa, Pieter Wesseling
Journal: Neuro-Oncology (2023)

Journal Article Unraveling schwannomas Get access Uta E Flucke, Uta E Flucke Princess Máxima Center for Pediatric Oncology, Utrecht, The NetherlandsDepartment of Pathology, Radboud University Medical Center, Nijmegen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar Laura S Hiemcke-Jiwa, Laura S Hiemcke-Jiwa Princess Máxima Center for Pediatric Oncology, Utrecht, The NetherlandsDepartment of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar Pieter Wesseling Pieter Wesseling Princess Máxima Center for Pediatric Oncology, Utrecht, The NetherlandsDepartment of Pathology, Amsterdam University Medical Centers, Amsterdam, The Netherlands Corresponding Author: Pieter Wesseling, MD PhD, Department of Pathology, Amsterdam University Medical Centers, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands (p.wesseling@amsterdamumc.nl) https://orcid.org/0000-0001-5453-5201 Search for other works by this author on: Oxford Academic PubMed Google Scholar Neuro-Oncology, Volume 25, Issue 12, December 2023, Pages 2237–2238, https://doi.org/10.1093/neuonc/noad171 Published: 05 October 2023 Article history Corrected and typeset: 05 October 2023 Published: 05 October 2023
Hidradenitis suppurativa: new insights into disease mechanisms and an evolving treatment landscape
Authors: James G Krueger, John Frew, Gregor B E Jemec, Alexa B Kimball, Brian Kirby, Falk G Bechara, Kristina Navrazhina, Errol Prens, Kristian Reich, Eva Cullen, Kerstin Wolk
Journal: British Journal of Dermatology (2023)

Abstract Hidradenitis suppurativa (HS), also known as acne inversa, is a chronic disabling and debilitating inflammatory disease with a high unmet medical need. The prevalence of HS reported in most studies is 1–2%, although it is likely to be under-reported and estimates vary globally owing to variance in data collection methods, ethnicity, geographical location and under-diagnosis. HS is characterized by persistent, painful cutaneous nodules, abscesses and draining tunnels commonly affecting the axillary, anogenital, inguinal and perianal/gluteal areas. Over time, chronic uncontrolled inflammation results in irreversible tissue destruction and scarring. Although the pathophysiology of HS has not been fully elucidated, the tumour necrosis factor (TNF)-α and interleukin (IL)-17 pathways have an important role, involving multiple cytokines. Currently, treatment options include topical medications; systemic therapies, including repeated and/or rotational courses of systemic antibiotics, retinoids and hormonal therapies; and various surgical procedures. The anti-TNF-α antibody adalimumab is currently the only biologic approved by both the US Food and Drug Administration and the European Medicines Agency for HS; however, its efficacy varies, with a clinical response reported in approximately 50% of patients in phase III trials. HS is a rapidly evolving field of discovery, with a diverse range of agents with distinct mechanisms of action currently being explored in clinical trials. Several other promising therapeutic targets have recently emerged, and agents targeting the IL-17 and Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathways are the most advanced in ongoing or completed phase III clinical trials. Alongside limited therapeutic options, significant challenges remain in terms of diagnosis and disease management, with a need for better treatment outcomes. Other unmet needs include significant diagnostic delays, thus missing the therapeutic ‘window of opportunity’; the lack of standardized outcome measures in clinical trials; and the lack of established, well-defined disease phenotypes and biomarkers.
NLGN4X TCR transgenic T cells to treat gliomas
Authors: Christoper Krämer, Michael Kilian, Yu-Chan Chih, Alexandros Kourtesakis, Dirk C Hoffmann, Tamara Boschert, Philipp Koopmann, Khwab Sanghvi, Alice De Roia, Stefanie Jung, Kristine Jähne, Bryan Day, Lenny D Shultz, Miriam Ratliff, Richard Harbottle, Edward W Green, Rainer Will, Wolfgang Wick, Michael Platten, Lukas Bunse
Journal: Neuro-Oncology (2023)

Neuroligin 4 X-linked (NLGN4X) harbors a human leukocyte antigen (HLA)-A*02-restricted tumor-associated antigen, overexpressed in human gliomas, that was found to induce specific cytotoxic T cell responses following multi-peptide vaccination in patients with newly diagnosed glioblastoma. T cell receptor (TCR) discovery was performed using droplet-based single-cell TCR sequencing of NLGN4X-tetramer-sorted T cells postvaccination. The identified TCR was delivered to Jurkat T cells and primary human T cells (NLGN4X-TCR-T). Functional profiling of NLGN4X-TCR-T was performed by flow cytometry and cytotoxicity assays. Therapeutic efficacy of intracerebroventricular NLGN4X-TCR-T was assessed in NOD scid gamma (NSG) major histocompatibility complex (MHC) I/II knockout (KO) (NSG MHC I/II KO) mice bearing NLGN4X-expressing experimental gliomas. An HLA-A*02-restricted vaccine-induced T cell receptor specifically binding NLGN4X131-139 was applied for preclinical therapeutic use. Reactivity, cytotoxicity, and polyfunctionality of this NLGN4X-specific TCR are demonstrated in various cellular models. Intracerebroventricular administration of NLGN4X-TCR-T prolongs survival and leads to an objective response rate of 44.4% in experimental glioma-bearing NSG MHC I/II KO mice compared to 0.0% in control groups. NLGN4X-TCR-T demonstrate efficacy in a preclinical glioblastoma model. On a global scale, we provide the first evidence for the therapeutic retrieval of vaccine-induced human TCRs for the off-the-shelf treatment of glioblastoma patients.Keywords cell therapy | glioblastoma | T cell receptor | tumor antigen.
Exploring the Potential of Nanogels: From Drug Carriers to Radiopharmaceutical Agents
Authors: Manja Kubeil, Yota Suzuki, Maria Antonietta Casulli, Rozy Kamal, Takeshi Hashimoto, Michael Bachmann, Takashi Hayashita, Holger Stephan
Journal: Advanced Healthcare Materials (2023)

Nanogels open up access to a wide range of applications and offer among others hopeful approaches for use in the field of biomedicine. This review provides a brief overview of current developments of nanogels in general, particularly in the fields of drug delivery, therapeutic applications, tissue engineering, and sensor systems. Specifically, cyclodextrin (CD)-based nanogels are important because they have exceptional complexation properties and are highly biocompatible. Nanogels as a whole and CD-based nanogels in particular can be customized in a wide range of sizes and equipped with a desired surface charge as well as containing additional molecules inside and outside, such as dyes, solubility-mediating groups or even biological vector molecules for pharmaceutical targeting. Currently, biological investigations are mainly carried out in vitro, but more and more in vivo applications are gaining importance. Modern molecular imaging methods are increasingly being used for the latter. Due to an extremely high sensitivity and the possibility of obtaining quantitative data on pharmacokinetic and pharmacodynamic properties, nuclear methods such as single photon emission computed tomography (SPECT) and positron emission tomography (PET) using radiolabeled compounds are particularly suitable here. The use of radiolabeled nanogels for imaging, but also for therapy, is being discussed.
High‐Performance Artificial Ligament Made from Helical Polyester Fibers Wrapped with Aligned Carbon Nanotube Sheets
Authors: Liyuan Wang, Hongyu Jiang, Fang Wan, Hongji Sun, Yiqing Yang, Wenjun Li, Zheyan Qian, Xuemei Sun, Peining Chen, Shiyi Chen, Huisheng Peng
Journal: Advanced Healthcare Materials (2023)

Repairing high-load connective tissues, such as ligaments, by surgically implanting artificial grafts after injury is challenging because they lack biointegration with host bones for stable interfaces. Herein, a high-performance helical composite fiber (HCF) ligament by wrapping aligned carbon nanotube (CNT) sheets around polyester fibers is proposed. Anterior cruciate ligament (ACL) reconstruction surgery shows that HCF grafts could induce effective bone regeneration, thus allowing the narrowing of bone tunnel defects. Such repair of the bone tunnel is in strong contrast to the tunnel enlargement of more than 50% for commercial artificial ligaments made from bare polyester fibers. Rats reconstructed with this HCF ligament show normal jumping, walking, and running without limping. This work allows bone regeneration in vivo through a one-step surgery without seeding cells or transforming growth factors, thereby opening an avenue for high-performance artificial tissues.
Near Infrared Emissive Lanthanide Luminescence Nanoparticle Used in Early Diagnosis and Brain Temperature Detection for Ischemic Stroke
Authors: Shen‐Jie Li, Fang Li, Na Kong, Jian‐Ren Liu, Xingjun Zhu
Journal: Advanced Healthcare Materials (2023)

Ischemic stroke (IS) is one of the most dangerous medical conditions resulting in high mortality and morbidity. The increased brain temperature after IS is closely related to prognosis, making it highly significant for the early diagnosis and the progression evaluation of IS. Herein, a temperature-responsive near infrared (NIR) emissive lanthanide luminescence nanoparticle is developed for the early diagnosis and brain temperature detection of IS. After intravenous injection, the nanoparticles can pass through the damaged blood-brain barrier of the ischemic region, allowing the extravasation and enrichment of nanoparticles into the ischemic brain tissue. The NIR luminescence signals of the nanoparticles are used not only to judge the location and severity of the cerebral ischemic injury but also to report the brain temperature variation in the ischemic area through a visualized way. The results show that the designed nanoparticles can be used for the early diagnosis of ischemic stroke and minimally invasive temperature detection of cerebral ischemic tissues in transient middle cerebral artery occlusion mice model, which is expected to make the clinical diagnosis of ischemic stroke more rapid and convenient, more accurately evaluate the state of brain injury in stroke patients and also guide stroke hypothermia treatment.
Feeding Next‐Generation Nanomedicines to Europe: Regulatory and Quality Challenges
Authors: Umberto M. Musazzi, Silvia Franzè, Fabrizio Condorelli, Paola Minghetti, Paolo Caliceti
Journal: Advanced Healthcare Materials (2023)

Abstract New and innovative nanomedicines have been developed and marketed over the past half‐century, revolutionizing the prognosis of many human diseases. Although a univocal regulatory definition is not yet available worldwide, the term “nanomedicines” generally identifies medicinal products that use nanotechnology in their design or production. Due to the intrinsic high structural complexity of these products, the scientific and regulatory communities are reflecting on how to revise the regulatory framework to provide a more appropriate benefit/risk balance to authorize them on the market, considering the impact of their peculiar physicochemical features in the evaluation of efficacy and safety patterns. Herein, a critical perspective is provided on the current open issues regarding regulatory qualification and physicochemical characterization of nanosystems considering the current European regulatory framework on nanomedicine products. Practicable paths for improving their quality assurance and predicting their fate in vivo are also argued. Strengthening the multilevel alliance among academic institutions, industrial stakeholders, and regulatory authorities seems strategic to support innovation by standard approaches (e.g., qualification, characterization, risk assessment), and to expand current knowledge, also benefiting from the new opportunities offered by artificial intelligence and digitization in predictive modelling of the impact of nanomedicine characteristics on their fate in vivo.
Mechanisms underlying the cell-matrixed nerve grafts repairing peripheral nerve defects
Authors: Shanshan Wang, Hongkui Wang, Panjian Lu, Leilei Gong, Xiaosong Gu, Meiyuan Li
Journal: Bioactive Materials (2023)

Decellularized extracellular matrix (dECM), with its distinct biological properties, has gained significant attention as a natural biomaterial. Leveraging its potentials, we successfully developed a three-dimensional matrix-based oriented nerve graft by encapsulating a fibrous scaffold with multilayered conformationally intact and biologically active human bone marrow mesenchymal stem cell-derived decellularized extracellular matrix (hBMSC-dECM). Convincingly, the hBMSC-dECM group exhibited comparable functional recoveries to the autograft group by postoperative week 12. In the comprehensive analysis, the molecular regulations in the hBMSC-dECM group were more intricate and nuanced compared to the autograft group. Nevertheless, both groups displayed similar molecular regulatory processes in terms of vascularization and extracellular matrix. Notably, the hBMSC-dECM group demonstrated sustained high levels of regulation in axon and myelin regeneration at week 12, while the immunomodulation returned to the normal levels after peaking at week 2. Collectively, our findings illustrated the satisfactory construction of a cell-matrixed nerve graft that established a microenvironment conducive to nerve regeneration, and elucidated the distinct molecular regulation patterns and characteristics associated with different repair modes.
Corrigendum to “EASL clinical practice guidelines on the management of cystic liver diseases” [J Hepatol (77) 1083–1108]
Authors: Joost Drenth, Thijs Barten, Tobias Böttler, Hermien Hartog, Frederik Nevens, Richard Taubert, Roser Torra Balcells, Valerie Vilgrain
Journal: Journal of Hepatology (2023)
Magnetically-targetable outer-membrane vesicles for sonodynamic eradication of antibiotic-tolerant bacteria in bacterial meningitis
Authors: Rui Shi, Rui Lv, Ziliang Dong, Qinghua Cao, Renfei Wu, Sidi Liu, Yijin Ren, Zhuang Liu, Henny C. van der Mei, Jian Liu, Henk J. Busscher
Journal: Biomaterials (2023)

Treatment of acute bacterial meningitis is difficult due to the impermeability of the blood-brain barrier, greatly limiting the antibiotic concentrations that can be achieved in the brain. Escherichia coli grown in presence of iron-oxide magnetic nanoparticles secrete large amounts of magnetic outer-membrane vesicles (OMVs) in order to remove excess Fe from their cytoplasm. OMVs are fully biomimetic nanocarriers, but can be inflammatory. Here, non-inflammatory magnetic OMVs were prepared from an E. coli strain in which the synthesis of inflammatory lipid A acyltransferase was inhibited using CRISPR/Cas9 mediated gene knockout. OMVs were loaded with ceftriaxone (CRO) and meso-tetra-(4-carboxyphenyl)porphine (TCPP) and magnetically driven across the blood-brain barrier for sonodynamic treatment of bacterial meningitis. ROS-generation upon ultrasound application of CRO- and TCPP-loaded OMVs yielded similar ROS-generation as by TCPP in solution. In vitro, ROS-generation by CRO- and TCPP-loaded OMVs upon ultrasound application operated synergistically with CRO to kill a hard-to-kill, CRO-tolerant E. coli strain. In a mouse model of CRO-tolerant E. coli meningitis, CRO- and TCPP-loaded OMVs improved survival rates and clinical behavioral scores of infected mice after magnetic targeting and ultrasound application. Recurrence did not occur for at least two weeks after arresting treatment.
TBC1D15 deficiency protects against doxorubicin cardiotoxicity via inhibiting DNA-PKcs cytosolic retention and DNA damage
Authors: Wenjun Yu, Haixia Xu, Zhe Sun, Yuxin Du, Shiqun Sun, Miyesaier Abudureyimu, Mengjiao Zhang, Jun Tao, Junbo Ge, Jun Ren, Yingmei Zhang
Journal: Acta Pharmaceutica Sinica B (2023)

Clinical application of doxorubicin (DOX) is heavily hindered by DOX cardiotoxicity. Several theories were postulated for DOX cardiotoxicity including DNA damage and DNA damage response (DDR), although the mechanism(s) involved remains to be elucidated. This study evaluated the potential role of TBC domain family member 15 (TBC1D15) in DOX cardiotoxicity. Tamoxifen-induced cardiac-specific Tbc1d15 knockout (Tbc1d15CKO) or Tbc1d15 knockin (Tbc1d15CKI) male mice were challenged with a single dose of DOX prior to cardiac assessment 1 week or 4 weeks following DOX challenge. Adenoviruses encoding TBC1D15 or containing shRNA targeting Tbc1d15 were used for Tbc1d15 overexpression or knockdown in isolated primary mouse cardiomyocytes. Our results revealed that DOX evoked upregulation of TBC1D15 with compromised myocardial function and overt mortality, the effects of which were ameliorated and accentuated by Tbc1d15 deletion and Tbc1d15 overexpression, respectively. DOX overtly evoked apoptotic cell death, the effect of which was alleviated and exacerbated by Tbc1d15 knockout and overexpression, respectively. Meanwhile, DOX provoked mitochondrial membrane potential collapse, oxidative stress and DNA damage, the effects of which were mitigated and exacerbated by Tbc1d15 knockdown and overexpression, respectively. Further scrutiny revealed that TBC1D15 fostered cytosolic accumulation of the cardinal DDR element DNA-dependent protein kinase catalytic subunit (DNA-PKcs). Liquid chromatography-tandem mass spectrometry and co-immunoprecipitation denoted an interaction between TBC1D15 and DNA-PKcs at the segment 594-624 of TBC1D15. Moreover, overexpression of TBC1D15 mutant (∆594-624, deletion of segment 594-624) failed to elicit accentuation of DOX-induced cytosolic retention of DNA-PKcs, DNA damage and cardiomyocyte apoptosis by TBC1D15 wild type. However, Tbc1d15 deletion ameliorated DOX-induced cardiomyocyte contractile anomalies, apoptosis, mitochondrial anomalies, DNA damage and cytosolic DNA-PKcs accumulation, which were canceled off by DNA-PKcs inhibition or ATM activation. Taken together, our findings denoted a pivotal role for TBC1D15 in DOX-induced DNA damage, mitochondrial injury, and apoptosis possibly through binding with DNA-PKcs and thus gate-keeping its cytosolic retention, a route to accentuation of cardiac contractile dysfunction in DOX-induced cardiotoxicity.
Comment on: “Interim results from the PEDISTAD Real-World Registry by Paller et al”—First results from the German TREATkids registry for children and adolescents with moderate-to-severe atopic dermatitis
Authors: Hagen Ott, Susanne Abraham, Barbara Kind, Eva Haufe, Stephan Weidinger, Thomas Werfel, Jochen Schmitt
Journal: Journal of the American Academy of Dermatology (2023)