tbuckley/cabot-search huggingface dataset:
Use the Receiver Operating Characteristic to Assess Model Accuracy—Reply
Authors: Xuan Wang, Brian Lee Claggett, Lu Tian
Journal: JAMA Cardiology (2023)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Cardiology HomeNew OnlineCurrent IssueFor Authors Podcast Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Cardiology journal
Association of Genetically Predicted Insomnia With Risk of Sepsis
Authors: Marianne S. Thorkildsen, Lise T. Gustad, Randi M. Mohus, Stephen Burgess, Tom I. L. Nilsen, Jan K. Damås, Tormod Rogne
Journal: JAMA Psychiatry (2023)

Insomnia has been associated with altered inflammatory response as well as increased risk of infections and sepsis in observational studies. However, these studies are prone to bias, such as residual confounding. To further understand the potential causal association between insomnia and sepsis risk, a 2-sample Mendelian randomization (MR) approach should be explored.
Four‐Arm Polymer‐Guided Formation of Curcumin‐Loaded Flower‐Like Porous Microspheres as Injectable Cell Carriers for Diabetic Wound Healing
Authors: Xiaohuan Yuan, Wei Yang, Yingying Fu, Ziwei Tao, Lan Xiao, Qinzhou Zheng, Dan Wu, Mengya Zhang, Luxin Li, Zhengmao Lu, Yan Wu, Jie Gao, Yulin Li
Journal: Advanced Healthcare Materials (2023)

Stem cell injection is an effective approach for treating diabetic wounds; however, shear stress during injections can negatively affect their stemness and cell growth. Cell-laden porous microspheres can provide shelter for bone mesenchymal stem cells (BMSC). Herein, curcumin-loaded flower-like porous microspheres (CFPM) are designed by combining phase inversion emulsification with thermally induced phase separation-guided four-arm poly (l-lactic acid) (B-PLLA). Notably, the CFPM shows a well-defined surface topography and inner structure, ensuring a high surface area to enable the incorporation and delivery of a large amount of -BMSC and curcumin. The BMSC-carrying CFPM (BMSC@CFPM) maintains the proliferation, retention, and stemness of -BMSCs, which, in combination with their sustainable curcumin release, facilitates the endogenous production of growth/proangiogenic factors and offers a local anti-inflammatory function. An in vivo bioluminescence assay demonstrates that BMSC@CFPM can significantly increase the retention and survival of BMSC in wound sites. Accordingly, BMSC@CFPM, with no significant systemic toxicity, could significantly accelerate diabetic wound healing by promoting angiogenesis, collagen reconstruction, and M2 macrophage polarization. RNA sequencing further unveils the mechanisms by which BMSC@CFPM promotes diabetic wound healing by increasing -growth factors and enhancing angiogenesis through the JAK/STAT pathway. Overall, BMSC@CFPM represents a potential therapeutic tool for diabetic wound healing.
Association of Transport Time, Proximity, and Emergency Department Pediatric Readiness With Pediatric Survival at US Trauma Centers
Authors: Nina E. Glass, Apoorva Salvi, Ran Wei, Amber Lin, Susan Malveau, Jennifer N. B. Cook, N. Clay Mann, Randall S. Burd, Peter C. Jenkins, Matthew Hansen, Nicholas M. Mohr, Caroline Stephens, Mary E. Fallat, E. Brooke Lerner, Brendan G. Carr, Stephen P. Wall, Craig D. Newgard
Journal: JAMA Surgery (2023)

Emergency department (ED) pediatric readiness is associated with improved survival among children. However, the association between geographic access to high-readiness EDs in US trauma centers and mortality is unclear.
JAMA Cardiology
Authors: Patrick O'gara, Marc Sabatine, Clyde Boston, Ann Yancy, Michael Pencina, Carolina North, Robert Harrington, Adrian Hernandez, Mhs Durham, North Carolina, Ajay Kirtane, New York, Roxana Mehran, Pradeep Natarajan, Kristen Boston, Seattle Patton, Washington Udelson, Sadiya Khan, Gregg Fonarow, Los Angeles, Sharlene Day, Laine Thomas, Akhil Narang, Vinay Guduguntla, San Francisco, Julia Getts, Eugene Braunwald, Judith Hochman, Donald Lloyd-Jones, Scm Chicago, Michael Parmacek, Daniel Simon, Sidney Smith, Chapel Hill, Carolina Douglas, E Vaughan, Myron Weisfeldt, Maryland Douglas, P Zipes, Paul Armstrong, Shinya Goto, Peter Liu, Renato Lopes, Carolina Prabhakaran, Hugh Watkins, Harvey White, Habib Yacoub, Frs London, Gregory Curfman, Annette Flanagin, Michael Berkwits, Brenda Gregoline, Tracy Frey, Iris Lo, Kevin Brown, Bernadette Hromin, Erin Keenan, Rebecca Langley, Kris Simmons, Connie Manno, Sara Billings, Doug Brandt, Timothy Gray, Peter Olson, Juliet Orellana, Paul Ruich, Kirby Snell, Judith Ahlers, Stephanie Beckmeyer, Dawn Bielawski, Jane Calayag, Jan Clavey, Mary Coerver-Connolly, Amy Eutsey, Paul Jaworski, Laura King, Mary Kingzette, Martha Lentz, Jenny Mackay, Jennifer Maybin, Charon Pierson, Shilpa Shenvi, Thressa Smith, Divya Sreekumar, Donna Thordsen, Christine Tilles, Emma Underdown, Cara Wallace, Becki Weiss, Christina West, Caroline Woods, Erin Kato, Jacob Kendall-Taylor, Monica Mungle, Lenette Gardner-Gullens, Anna Bukowski, Lee Calibeo, Jordan Faber, Andrew Given, Tosca Hall, Emma Hyche, Kenneth Otani, Caroline Sietmann, Angelia Digital, Elena Guobyte, R Lile, Daniel Morrow, Daniel Musisi, Shelly Steff, Jennifer Zeis, Reuben Rios, Mary Ferkaluk, Dawn Cortez, Sidra Bonner, Heather Hsu, Andrew Ibrahim, Karen Bucher, Andy Rekito, Nick Reback, Lohitha Kethu, Marla Erson, Joseph Thornton, Thomas Easley, Brian Shields, Karen Adams-Taylor, Paul Gee, Anna Frazier, Monica Smith, Julie Burton, Ronna Hewitt, Mary Sieck, Geneine Someren, Jamaal Vargas, Content Systems, Mike Bujewski, Nathan Mitre, Digital Production, Regina Brownlee, Amanda Camino, Brenda Chandler-Grayer, Emily Dunn, Amy Evers, Maleeka Holden, Teri Hutchison, Dan Knowles, Joshua Lampinen, Natalie Marsh, Patricia Panek, Marla Seidell, La Son, Diggs Sledge, Julian Graphics, Joe Amft, Jonathan Chala, Maria Duda, Fred Furtner, Carolyn Hall, Maria Kowalkowski, Kelley Mcdonald, Joy Farrell, Maria Giannini, Sasha Grossman, Kate Lander, Judith Literskis, Teresa Omiotek, Michael Ryder, James Madara, Kirsten Bibbins-Domingo, Robert Bonow
Journal: JAMA Cardiology (2023)

science, and evidence-based health policy.Health equity, especially when supported by original science, is a leading editorial priority.
Use the Receiver Operating Characteristic to Assess Model Accuracy
Authors: Harry B. Burke
Journal: JAMA Cardiology (2023)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Cardiology HomeNew OnlineCurrent IssueFor Authors Podcast Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Cardiology journal
A metabolite from commensal Candida albicans enhances the bactericidal activity of macrophages and protects against sepsis
Authors: Peng Gu, Ruofan Liu, Qin Yang, Li Xie, Rongjuan Wei, Jiaxin Li, Fengyi Mei, Tao Chen, Zhenhua Zeng, Yan He, Hongwei Zhou, Hongjuan Peng, Kutty Selva Nandakumar, Huikuan Chu, Yong Jiang, Wei Gong, Ye Chen, Bernd Schnabl, Peng Chen
Journal: Cellular and Molecular Immunology (2023)
Germline Variants Incidentally Detected via Tumor-Only Genomic Profiling of Patients With Mesothelioma
Authors: Owen D. Mitchell, Katie Gilliam, Daniela del Gaudio, Kelsey E. McNeely, Shaili Smith, Maria Acevedo, Meghana Gaduraju, Rachel Hodge, Aubrianna S. S. Ramsland, Jeremy Segal, Soma Das, Feighanne Hathaway, Darren S. Bryan, Sanjukta Tawde, Shelly Galasinski, Peng Wang, Melissa Y. Tjota, Aliya N. Husain, Samuel G. Armato, Jessica Donington, Mark K. Ferguson, Kiran Turaga, Jane E. Churpek, Hedy L. Kindler, Michael W. Drazer
Journal: JAMA Network Open (2023)

Importance Patients with mesothelioma often have next-generation sequencing (NGS) of their tumor performed; tumor-only NGS may incidentally identify germline pathogenic or likely pathogenic (P/LP) variants despite not being designed for this purpose. It is unknown how frequently patients with mesothelioma have germline P/LP variants incidentally detected via tumor-only NGS. Objective To determine the prevalence of incidental germline P/LP variants detected via tumor-only NGS of mesothelioma. Design, Setting, and Participants A series of 161 unrelated patients with mesothelioma from a high-volume mesothelioma program had tumor-only and germline NGS performed during April 2016 to October 2021. Follow-up ranged from 18 months to 7 years. Tumor and germline assays were compared to determine which P/LP variants identified via tumor-only NGS were of germline origin. Data were analyzed from January to March 2023. Main Outcomes and Measures The proportion of patients with mesothelioma who had P/LP germline variants incidentally detected via tumor-only NGS. Results Of 161 patients with mesothelioma, 105 were male (65%), the mean (SD) age was 64.7 (11.2) years, and 156 patients (97%) self-identified as non-Hispanic White. Most (126 patients [78%]) had at least 1 potentially incidental P/LP germline variant. The positive predictive value of a potentially incidental germline P/LP variant on tumor-only NGS was 20%. Overall, 26 patients (16%) carried a P/LP germline variant. Germline P/LP variants were identified in ATM , ATR , BAP1 , CHEK2, DDX41 , FANCM , HAX1 , MRE11A , MSH6 , MUTYH , NF1 , SAMD9L , and TMEM127 . Conclusions and Relevance In this case series of 161 patients with mesothelioma, 16% had confirmed germline P/LP variants. Given the implications of a hereditary cancer syndrome diagnosis for preventive care and familial counseling, clinical approaches for addressing incidental P/LP germline variants in tumor-only NGS are needed. Tumor-only sequencing should not replace dedicated germline testing. Universal germline testing is likely needed for patients with mesothelioma.
GABA Regulates Electrical Activity and Tumor Initiation in Melanoma
Authors: Mohita Tagore, Emiliano Hergenreder, Sarah C. Perlee, Nelly M. Cruz, Laura Menocal, Shruthy Suresh, Eric Chan, Maayan Baron, Stephanie Melendez, Asim Dave, Walid K. Chatila, Jeremie Nsengimana, Richard P. Koche, Travis J. Hollmann, Trey Ideker, Lorenz Studer, Andrea Schietinger, Richard M. White
Journal: Cancer Discovery (2023)

Abstract Oncogenes can initiate tumors only in certain cellular contexts, which is referred to as oncogenic competence. In melanoma, whether cells in the microenvironment can endow such competence remains unclear. Using a combination of zebrafish transgenesis coupled with human tissues, we demonstrate that GABAergic signaling between keratinocytes and melanocytes promotes melanoma initiation by BRAFV600E. GABA is synthesized in melanoma cells, which then acts on GABA-A receptors in keratinocytes. Electron microscopy demonstrates specialized cell–cell junctions between keratinocytes and melanoma cells, and multielectrode array analysis shows that GABA acts to inhibit electrical activity in melanoma/keratinocyte cocultures. Genetic and pharmacologic perturbation of GABA synthesis abrogates melanoma initiation in vivo. These data suggest that GABAergic signaling across the skin microenvironment regulates the ability of oncogenes to initiate melanoma. Significance: This study shows evidence of GABA-mediated regulation of electrical activity between melanoma cells and keratinocytes, providing a new mechanism by which the microenvironment promotes tumor initiation. This provides insights into the role of the skin microenvironment in early melanomas while identifying GABA as a potential therapeutic target in melanoma. See related commentary by Ceol, p. 2128. This article is featured in Selected Articles from This Issue, p. 2109
Evaluation of Professional Setbacks and Resilience in Biomedical Scientists During the COVID-19 Pandemic
Authors: Nicole C. Woitowich, Anthony C. Waddimba, Chen Yeh, Lutfiyya N. Muhammad, Ann Marie Warren, Christine V. Wood
Journal: JAMA Network Open (2023)

This survey study evaluates whether resilience was associated with professional and career setbacks among biomedical scientists during the COVID-19 pandemic.
Autoreactive IgE: Pathogenic role and therapeutic target in autoimmune diseases
Authors: Nicolas Charles, Inge Kortekaas‐Krohn, Emek Kocaturk, Jörg Scheffel, Sabine Altrichter, Carolin Steinert, Yi‐Kui Xiang, Jan Gutermuth, Laurent L. Reber, Marcus Maurer
Journal: Allergy (2023)

Abstract Autoimmunity is the break of tolerance to self‐antigens that leads to organ‐specific or systemic diseases often characterized by the presence of pathogenic autoreactive antibodies (AAb) produced by plasmablast and/or plasma cells. AAb are prevalent in the general population and not systematically associated with clinical symptoms. In contrast, in some individuals, these AAb are pathogenic and drive the development of signs and symptoms of antibody‐mediated autoimmune diseases (AbAID). AAb production, isotype profiles, and glycosylations are promoted by pro‐inflammatory triggers linked to genetic, environmental, and hormonal parameters. Recent evidence supports a role for pathogenic AAb of the IgE isotype in a number of AbAID. Autoreactive IgE can drive the activation of mast cells, basophils, and other types of FcεRI‐bearing cells and may play a role in promoting autoantibody production and other pro‐inflammatory pathways. In this review, we discuss the current knowledge on the pathogenicity of autoreactive IgE in AbAID and their status as therapeutic targets. We also highlight unresolved issues including the need for assays that reproducibly quantify IgE AAbs, to validate their diagnostic and prognostic value, and to further study their pathophysiological contributions to AbAID.
SparkMaster 2: A New Software for Automatic Analysis of Calcium Spark Data
Authors: Jakub Tomek, Madeline Nieves-Cintron, Manuel F. Navedo, Christopher Y. Ko, Donald M. Bers
Journal: Circulation Research (2023)

Calcium (Ca) sparks are elementary units of subcellular Ca release in cardiomyocytes and other cells. Accordingly, Ca spark imaging is an essential tool for understanding the physiology and pathophysiology of Ca handling and is used to identify new drugs targeting Ca-related cellular dysfunction (eg, cardiac arrhythmias). The large volumes of imaging data produced during such experiments require accurate and high-throughput analysis.We developed a new software tool SparkMaster 2 (SM2) for the analysis of Ca sparks imaged by confocal line-scan microscopy, combining high accuracy, flexibility, and user-friendliness. SM2 is distributed as a stand-alone application requiring no installation. It can be controlled using a simple-to-use graphical user interface, or using Python scripting.SM2 is shown to have the following strengths: (1) high accuracy at identifying Ca release events, clearly outperforming previous highly successful software SparkMaster; (2) multiple types of Ca release events can be identified using SM2: Ca sparks, waves, miniwaves, and long sparks; (3) SM2 can accurately split and analyze individual sparks within spark clusters, a capability not handled adequately by prior tools. We demonstrate the practical utility of SM2 in two case studies, investigating how Ca levels affect spontaneous Ca release, and how large-scale release events may promote release refractoriness. SM2 is also useful in atrial and smooth muscle myocytes, across different imaging conditions.SparkMaster 2 is a new, much-improved user-friendly software for accurate high-throughput analysis of line-scan Ca spark imaging data. It is free, easy to use, and provides valuable built-in features to facilitate visualization, analysis, and interpretation of Ca spark data. It should enhance the quality and throughput of Ca spark and wave analysis across cell types, particularly in the study of arrhythmogenic Ca release events in cardiomyocytes.
Memory-like Differentiation, Tumor-Targeting mAbs, and Chimeric Antigen Receptors Enhance Natural Killer Cell Responses to Head and Neck Cancer
Authors: Miriam T. Jacobs, Pamela Wong, Alice Y. Zhou, Michelle Becker-Hapak, Nancy D. Marin, Lynne Marsala, Mark Foster, Jennifer A. Foltz, Celia C. Cubitt, Jennifer Tran, David A. Russler-Germain, Carly Neal, Samantha Kersting-Schadek, Lily Chang, Timothy Schappe, Patrick Pence, Ethan McClain, Jose P. Zevallos, Jason T. Rich, Randal C. Paniello, Ryan S. Jackson, Patrik Pipkorn, Douglas R. Adkins, Carl J. DeSelm, Melissa M. Berrien-Elliott, Sidharth V. Puram, Todd A. Fehniger
Journal: Clinical Cancer Research (2023)

Head and neck squamous cell carcinoma (HNSCC) is an aggressive tumor with low response rates to frontline PD-1 blockade. Natural killer (NK) cells are a promising cellular therapy for T cell therapy-refractory cancers, but are frequently dysfunctional in patients with HNSCC. Strategies are needed to enhance NK cell responses against HNSCC. We hypothesized that memory-like (ML) NK cell differentiation, tumor targeting with cetuximab, and engineering with an anti-EphA2 (Erythropoietin-producing hepatocellular receptor A2) chimeric antigen receptor (CAR) enhance NK cell responses against HNSCC.
Triple G Agonists — A Home Run for Obesity?
Authors: Mary Elizabeth Patti
Journal: New England Journal of Medicine (2023)

The buzz was palpable at the 2023 American Diabetes Association Scientific Sessions as multiple new nutrient-stimulated, hormone-based therapies for obesity and type 2 diabetes took center stage. Among these therapies, the triple–hormone-receptor agonist retatrutide — the subject of a phase 2 trial by Jastreboff et al., the results of which are published in this issue of the Journal1 — showed unprecedented efficacy in treating obesity, with 24% weight loss over 48 weeks. Hormonal responses to nutrient intake are key regulators of metabolism. The best known is insulin secretion in response to postprandial increases in levels of glucose or amino acids, . . .
Standardising acute myeloid leukaemia classification systems: a perspective from a panel of international experts
Authors: Rory M Shallis, Naval Daver, Jessica K Altman, Rami S Komrokji, Daniel A Pollyea, Talha Badar, Jan P Bewersdorf, Vijaya R Bhatt, Stéphane de Botton, Adolfo de la Fuente Burguera, Hetty E Carraway, Pinkal Desai, Richard Dillon, Nicolas Duployez, Firas El Chaer, Amir T Fathi, Sylvie D Freeman, Ivana Gojo, Michael R Grunwald, Brian A Jonas, Marina Konopleva, Tara L Lin, Gabriel N Mannis, John Mascarenhas, Laura C Michaelis, Alice S Mims, Pau Montesinos, Olga Pozdnyakova, Keith W Pratz, Andre C Schuh, Mikkael A Sekeres, Catherine C Smith, Maximilian Stahl, Marion Subklewe, Geoffrey L Uy, Maria Teresa Voso, Roland B Walter, Eunice S Wang, Joshua F Zeidner, Andrius Žučenka, Amer M Zeidan
Journal: The Lancet Haematology (2023)
Developmental Selection and the Perception of Mutation Bias
Authors: Paco Majic, Joshua L Payne
Journal: Molecular Biology and Evolution (2023)

Abstract The notion that mutations are random relative to their fitness effects is central to the Neo-Darwinian view of evolution. However, a recent interpretation of the patterns of mutation accumulation in the genome of Arabidopsis thaliana has challenged this notion, arguing for the presence of a targeted DNA repair mechanism that causes a nonrandom association of mutation rates and fitness effects. Specifically, this mechanism was suggested to cause a reduction in the rates of mutations on essential genes, thus lowering the rates of deleterious mutations. Central to this argument were attempts to rule out selection at the population level. Here, we offer an alternative and parsimonious interpretation of the patterns of mutation accumulation previously attributed to mutation bias, showing how they can instead or additionally be caused by developmental selection, that is selection occurring at the cellular level during the development of a multicellular organism. Thus, the depletion of deleterious mutations in A. thaliana may indeed be the result of a selective process, rather than a bias in mutation. More broadly, our work highlights the importance of considering development in the interpretation of population-genetic analyses of multicellular organisms, and it emphasizes that efforts to identify mechanisms involved in mutational biases should explicitly account for developmental selection.
Interleukin-6 in Patients With Heart Failure and Preserved Ejection Fraction
Authors: Alessio Alogna, Katlyn E. Koepp, Michael Sabbah, Jair M. Espindola Netto, Michael D. Jensen, James L. Kirkland, Carolyn S.P. Lam, Masaru Obokata, Mark C. Petrie, Paul M. Ridker, Hidemi Sorimachi, Tamara Tchkonia, Adriaan Voors, Margaret M. Redfield, Barry A. Borlaug
Journal: JACC Heart Failure (2023)