tbuckley/cabot-search huggingface dataset:
Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology
Authors: Hana Stepankova, Hana Michalkova, Zbynek Splichal, Lukas Richtera, Pavel Svec, Tomas Vaculovic, Jan Pribyl, Martin Kormunda, Simona Rex, Vojtech Adam, Zbynek Heger
Journal: Bioactive Materials (2022)

Although the general concept of nanotechnology relies on exploitation of size-dependent properties of nanoscaled materials, the relation between the size/morphology of nanoparticles with their biological activity remains not well understood. Therefore, we aimed at investigating the biological activity of Se nanoparticles, one of the most promising candidates of nanomaterials for biomedicine, possessing the same crystal structure, but differing in morphology (nanorods vs. spherical particles) and aspect ratios (AR, 11.5 vs. 22.3 vs. 1.0) in human cells and BALB/c mice. Herein, we report that in case of nanorod-shaped Se nanomaterials, AR is a critical factor describing their cytotoxicity and biocompatibility. However, spherical nanoparticles (AR 1.0) do not fit this statement and exhibit markedly higher cytotoxicity than lower-AR Se nanorods. Beside of cytotoxicity, we also show that morphology and size substantially affect the uptake and intracellular fate of Se nanomaterials. In line with in vitro data, in vivo i.v. administration of Se nanomaterials revealed the highest toxicity for higher-AR nanorods followed by spherical nanoparticles and lower-AR nanorods. Moreover, we revealed that Se nanomaterials are able to alter intracellular redox homeostasis, and affect the acidic intracellular vesicles and cytoskeletal architecture in a size- and morphology-dependent manner. Although the tested nanoparticles were produced from the similar sources, their behavior differs markedly, since each type is promising for several various application scenarios, and the presented testing protocol could serve as a concept standardizing the biological relevance of the size and morphology of the various types of nanomaterials and nanoparticles.
Clinical implications of left atrial changes after optimization of medical therapy in patients with heart failure
Authors: Riccardo M. Inciardi, Matteo Pagnesi, Carlo M. Lombardi, Stefan D. Anker, John G. Cleland, Kenneth Dickstein, Gerasimos S. Filippatos, Chim C. Lang, Leong L. Ng, Pierpaolo Pellicori, Piotr Ponikowski, Nilesh J. Samani, Faiez Zannad, Dirk J. van Veldhuisen, Scott D. Solomon, Adriaan A. Voors, Marco Metra
Journal: European Journal of Heart Failure (2022)

Abstract Aims Limited data exist regarding the prognostic relevance of changes in left atrial (LA) dimensions in patients with heart failure (HF). We assessed changes in LA dimension and their relation with outcomes after optimization of guideline‐directed medical therapy (GDMT) in patients with new‐onset or worsening HF. Methods and results Left atrial diameter was assessed at baseline and 9 months after GDMT optimization in 632 patients (mean age 65.8 ± 12.1 years, 22.3% female) enrolled in BIOSTAT‐CHF. LA adverse remodelling (LAAR) was defined as an increase in LA diameter on transthoracic echocardiography between baseline and 9 months. After the 9‐month visit, patients were followed for a median of 13 further months. LAAR was observed in 247 patients (39%). Larger baseline LA diameter (odds ratio [OR] 0.90; 95% confidence interval [CI] 0.87–0.93; p &lt; 0.001) and up‐titration to higher doses of angiotensin‐converting enzyme inhibitors or angiotensin receptor blockers (ACEi/ARBs) (OR 0.56; 95% CI 0.34–0.92; p = 0.022) were independently associated with lower likelihood of LAAR. LAAR was associated with an increased risk of the composite of all‐cause mortality or HF hospitalization (log‐rank p = 0.007 and adjusted hazard ratio 1.73, 95% CI 1.22–2.45, p = 0.002). The association was more pronounced in patients without a history of atrial fibrillation ( p for interaction = 0.009). Conclusion Among patients enrolled in BIOSTAT‐CHF, LAAR was associated with an unfavourable outcome and was prevented by ACEi/ARB up‐titration. Changes in LA dimension may be a useful marker of response to treatment and improve risk stratification in patients with HF.
PB1771: THE SIGNIFICANCE OF MRD DETECTION BY FLOW CYTOMETRY IN TRACING R/R B-ALL PATIENTS RECEIVING CD-19-CART BRIDGING TO ALLO-HSCT
Authors: M. chen, W. zhao, M. fu, H. Wang
Journal: HemaSphere (2022)

Background: Minimal Residual Disease(MRD) detection by multicolor flow cytometry(MFC) is an important parameter for monitoring therapy effect in acute leukaemia. However, MFC panels for MRD detection after CD19-CAR-T treatment should change because the expression of CD19 will lose or partially lose. Aims: To discuss the significance of MRD detection by MFC with cytoplastic CD79a(cCD79a) gating panel in relapsed or refractory B-cell acute lymphoblastic leukemia(r/r B-ALL) and evaluate its value for prognosis in CD19-CAR-T bridging to allogeneic-hematopoietic stem cell transplantation (allo-HSCT). Methods: 37 r/r B-ALL patients who received CD-19-CAR-T infusion bridging to allo-HSCT were enrolled at Hebei Yanda Lu Daopei Hospital from January 2019 to July 2019. Patients had not been treated with CAR-T and/or allo-HSCT before. MFC is used to monitor the patient’s MRD and the dynamic of B cells. The median follow-up time of 37 patients after treatment was 890 days (104-1062). Binary logistic regression was used to retrospectively analyze the data. Results: 8 patients (8/37, 21.62%) died and 29 (29/37, 78.38%) survived. when using death/survival as the end point in the dependent variable, MFC-MRD-positive recurrence (P=0.0006), extramedullary infiltration (P=0.047), and chromosomal karyotype changes (P=0.047) showed significant differences between the two groups, as well as the remission rate of MRD on 15d (P=0.011), the remission rate of the MRD on the 28d (P=0.002), and the time from CD19 positive recovery to CAR-T before allo-HSCT (P=0.020) after CAR-T reinfusion to allo-HSCT. P values of these indicators were all less than 0.05. the rest factors were not significant between the two groups. In the binary Logistic regression model of related factors during treatment and disease outcome, FCM-MRD remission on 28d can be used as a potential indicator to evaluate disease death or survival outcome, while other factors are excluded from the equation. The area under the ROC curve for the analysis of disease prognosis affecting factors was 0.762 (P=0.026), OR=12.667, the sensitivity was 0.625, and the specificity was 0.897. Image:Summary/Conclusion: The cCD79a antibody gated MFC-MRD monitoring can play a certain guiding significance in the prognosis judgment of the disease and the selection of the type of CART.
Nanosilicate‐Reinforced Silk Fibroin Hydrogel for Endogenous Regeneration of Both Cartilage and Subchondral Bone
Authors: Renwang Sheng, Jialin Chen, Hongmei Wang, Yifan Luo, Jia Liu, Zhixuan Chen, Qingyun Mo, Jiayu Chi, Chen Ling, Xin Tan, Qingqiang Yao, Wei Zhang
Journal: Advanced Healthcare Materials (2022)

Abstract Osteochondral defects are characterized by injuries to both cartilage and subchondral bone, which is a result of trauma, inflammation, or inappropriate loading. Due to the unique biological properties of subchondral bone and cartilage, developing a tissue engineering scaffold that can promote dual‐lineage regeneration of cartilage and bone simultaneously remains a great challenge. In this study, a microporous nanosilicate‐reinforced enzymatically crosslinked silk fibroin (SF) hydrogel is fabricated by introducing montmorillonite (MMT) nanoparticles via intercalation chemistry. In vitro studies show that SF‐MMT nanocomposite hydrogel has improved mechanical properties and hydrophilicity, as well as the bioactivities to promote the osteogenic differentiation of bone marrow mesenchymal stem cells and maintain chondrocyte phenotype compared with SF hydrogel. Global proteomic analysis verifies the dual‐lineage bioactivities of SF‐MMT nanocomposite hydrogel, which are probably regulated by multiple signaling pathways. Furthermore, it is observed that the biophysical interaction of cells and SF‐MMT nanocomposite hydrogel is partially mediated by clathrin‐mediated endocytosis and its downstream processes. In vivo, the SF‐MMT nanocomposite hydrogel effectively promotes osteochondral regeneration as evidenced by macroscopic, micro‐CT, and histological evaluation. In conclusion, a functionalized SF‐MMT nanocomposite hydrogel is developed with dual‐lineage bioactivity for osteochondral regeneration, indicating its potential in osteochondral tissue engineering.
Construction of Rhodamine‐Based AIE Photosensitizer Hydrogel with Clinical Potential for Selective Ablation of Drug‐Resistant Gram‐Positive Bacteria In Vivo
Authors: Shuang Zeng, Zuokai Wang, Chen Chen, Xiaosheng Liu, Yu Wang, Qixian Chen, Jingyun Wang, Haidong Li, Xiaojun Peng, Juyoung Yoon
Journal: Advanced Healthcare Materials (2022)

Abstract The emergence of powerful antibiotic‐resistant bacteria caused by the abuse of antibiotics has become a public health problem. Photodynamic antibacterial therapy is regarded as an innovative and promising antibacterial approach due to its minor side effects and lack of drug resistance. Nevertheless, few photosensitizers (PSs) are reported to have near‐infrared (NIR) emission, the ability to rapidly discriminate bacteria, and high photodynamic antibacterial efficiency. In this study, it is reported for the first time that a water‐soluble NIR fluorescence emission rhodamine‐based photosensitizer with aggregation‐inducing emission (AIE) effects, referred to as CS‐2I, can efficiently identify and kill Gram‐positive bacteria. In a fluorescence imaging experiment with blended bacteria, CS‐2I can selectively target Gram‐positive bacteria and specifically label Gram‐positive bacteria with high efficiency after only 5 min of incubation. Furthermore, CS‐2I achieves complete inhibition of methicillin‐resistant Staphylococcus aureus (MRSA) at an extremely low concentration (0.5 µ m ) and light dosage (6 J cm −2 ). Remarkably, CS‐2I is mixed with Carbomer 940 to prepare an antibacterial hydrogel dressing (CS‐2I@gel), and in vitro and in vivo results demonstrate that CS‐2I@gel provides extraordinary performance in photodynamic antibacterial therapy. Hence, this study provides a new strategy and blueprint for the future design of antibacterial materials.
Single cell heterogeneity and evolution of breast cancer bone metastasis and organoids reveals therapeutic targets for precision medicine
Authors: K. Ding, F. Chen, N. Priedigkeit, D.D. Brown, K. Weiss, R. Watters, K.M. Levine, T. Heim, W. Li, J. Hooda, P.C. Lucas, J.M. Atkinson, S. Oesterreich, A.V. Lee
Journal: Annals of Oncology (2022)
Combined ERCP and endoscopic ultrasound-guided antegrade stenting for hilar biliary obstruction in a patient after pancreatoduodenectomy
Authors: Kazuma Ishikawa, Hirotoshi Ishiwatari, Fumitaka Niiya, Hiroyuki Matsubayashi, Hiroyuki Ono
Journal: Endoscopy (2022)

Biliary drainage of hilar biliary obstruction (HBO) after surgical biliary reconstruction, including pancreatoduodenectomy, is challenging because recurrent tumors hamper access to the hepaticojejunostomy anastomosis (HJA) [1]. Although endoscopic ultrasound (EUS)-guided hepaticogastrostomy is an alternative, biliary drainage of both hepatic lobes is still difficult [2] [3] [4]. We report a case in which multiple metal stents were placed across an unrecognizable HJA using a partial stent-in-stent technique with EUS-guided antegrade stenting.
An unexpected adverse event during gastrointestinal endoscopy involving retrieval of dislodged endoscopic suture material from the trachea
Authors: Daniyal Abbas, Nicholas McDonald, Mohammad Bilal
Journal: Endoscopy (2022)

A 75-year-old man with T3N1MO esophageal adenocarcinoma who was deemed to be a poor surgical candidate and was being evaluated for chemoradiotherapy was referred for progressive dysphagia and esophageal stent placement. An upper gastrointestinal (GI) endoscopy was performed, which showed a mass in the distal esophagus leading to near-complete obstruction of the esophageal lumen. A through-the-scope fully covered self-expandable metal stent (diameter 18 mm, length 9.7 cm; Boston Scientific, Marlborough, Massachusetts, USA) was placed. To prevent stent migration, it was planned that two endoscopic sutures would be placed for stent fixation in a "mucosa–stent–mucosa" fashion. Upon deployment of the second suture, there was a device malfunction and it was not possible to deploy the cinch. The suture was cut using endoscopic scissors; however, during this process, the endoscopic suturing device briefly got stuck in the stent retrieval thread, leading to proximal stent migration. As a result, the stent was removed.
Gel-immersion endoscopic detorsion for pediatric sigmoid volvulus
Authors: Yasunori Yamamoto, Yoshiou Ikeda, Eiji Takeshita, Toshihiro Jogamoto, Takahiro Motoki, Mariko Eguchi, Yoichi Hiasa
Journal: Endoscopy (2022)

Pediatric sigmoid volvulus is a rare but emergency disease caused by abnormal twisting of the bowel along the mesenteric axis [1] [2]. Water immersion colonoscopy has been reported to be effective for sigmoid volvulus because it minimizes colonic distension and improves visibility [3] [4]. Gel-immersion endoscopy is a new method for securing the visual field during endoscopy [5]. The gel is better than water for removing contaminated bowel fluid and improving visibility. Moreover, the weight of the gel opens the twisting colon and facilitates volvulus passage. We present a case of pediatric sigmoid volvulus treated with gel-immersion endoscopic detorsion.
Retracted: Targeting neddylation in cancer
Authors: Anshika Goenka, Shi-Yuan Cheng
Journal: Neuro-Oncology (2022)

Journal Article Retracted: Targeting neddylation in cancer Get access Anshika Goenka, Anshika Goenka The Ken & Ruth Davee Department of Neurology, Lou and Jean Malnati Brain Tumor Institute at Northwestern Medicine, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Shi-Yuan Cheng Shi-Yuan Cheng The Ken & Ruth Davee Department of Neurology, Lou and Jean Malnati Brain Tumor Institute at Northwestern Medicine, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA Corresponding Author: Shi-Yuan Cheng, PhD, The Ken & Ruth Davee Department of Neurology, Lou and Jean Malnati Brain Tumor Institute at Northwestern Medicine, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA (shiyuan.cheng@northwestern.edu). https://orcid.org/0000-0003-1737-0588 Search for other works by this author on: Oxford Academic PubMed Google Scholar Neuro-Oncology, Volume 24, Issue 11, November 2022, Pages 1869–1870, https://doi.org/10.1093/neuonc/noac159 Published: 24 June 2022 Article history Published: 24 June 2022 Corrected and typeset: 07 July 2022
Direct large flow of venous gas into right atrium and ventricle during endoscopic biliary treatment
Authors: Koki Hoshi, Keiichi Tominaga, Naoya Izawa, Akira Yamamiya, Kazunori Nagashima, Takahito Minaguchi, Atsushi Irisawa
Journal: Endoscopy (2022)

The patient, a 32-year-old woman, had undergone partial resection of the left lobe of the liver and extrahepatic bile duct with choledochojejunostomy for numerous intrahepatic biliary stones due to primary sclerosing cholangitis ([Fig. 1]). An afferent loop of jejunum–cutaneous fistula had also been surgically created for additional endoscopic treatment. Three months after surgery, we attempted endoscopic removal of the remaining stones using the jejunocutaneous fistula. An endoscope was inserted through the fistula to reach the biliary–jejunal fistula ([Fig. 2]). Choledochography showed multiple biliary strictures ([Fig. 3]). Several strictures were dilated with 1-mm to 8-mm balloons inflated using carbon dioxide ([Fig. 4]). When the balloon dilator was deflated and removed, fluoroscopy showed gas entering the right ventricle. No abnormal bleeding occurred from the bile duct. The endoscope was immediately removed from the jejunal–cutaneous fistula. Fluoroscopic investigation revealed no continuous leakage of gas into the ventricle ([Video 1]). Fortunately, computed tomography showed no sign of gas embolism in the various organs.
Unusual diagnosis of malignant melanoma via cholangioscopy
Authors: Martha M. Kirstein, Lars Tharun, Jens U. Marquardt, Philipp Solbach
Journal: Endoscopy (2022)

A 75-year-old male patient was admitted to the emergency department with febrile temperature. The patient presented with multiple, very dark nevi all over his face and body, previously diagnosed as benign.
POU2F3 in SCLC: Clinicopathologic and Genomic Analysis With a Focus on Its Diagnostic Utility in Neuroendocrine-Low SCLC
Authors: Marina K. Baine, Christopher A. Febres-Aldana, Jason C. Chang, Achim A. Jungbluth, Shenon Sethi, Cristina R. Antonescu, William D. Travis, Min-Shu Hsieh, Mee Sook Roh, Robert J. Homer, Marc Ladanyi, Jacklynn V. Egger, W. Victoria Lai, Charles M. Rudin, Natasha Rekhtman
Journal: Journal of Thoracic Oncology (2022)
Hydrogel Combined with Phototherapy in Wound Healing
Authors: Yinglin Xu, Haolin Chen, Yifen Fang, Jun Wu
Journal: Advanced Healthcare Materials (2022)

Abstract Wound healing is a complex biological process that involves tissue regeneration. Traditional wound dressings are dry, cannot provide a moist environment for wound healing, and do not have high antibacterial properties. Hydrogels, which are capable of retaining large amounts of water, can create a moist healing environment. Currently, phototherapies have exhibited a high potential for the treatment of bacterial infections. Therefore, combining hydrogels with phototherapy can adequately overcome the shortcomings of traditional wound treatment methods and show great potential for wound healing owing to their high efficiency, low irritation, and good antibacterial performance. In this review, the application of hydrogels combined with phototherapy in wound healing is summarized. First, the basic principles of photodynamic therapy and photothermal therapy are briefly introduced. In addition, the progress of the application of hydrogel combined with phototherapy in wound healing is systematically investigated. Finally, the challenges and prospects of combining hydrogel with phototherapy in wound healing are discussed.
PB1778: NB4 CELLS CAN SURVIVE DURING ATO TREATMENT BY REGULATING THE NUMBER OF MITOCHONDRIA
Authors: S. Ding, J. Wang, Y. Wang, S. Cui, Z. Li, X. Yin, J. Xu, K. Liu, R. Xu
Journal: HemaSphere (2022)

Background: Defects in mitochondrial dynamics are associated with many human diseases including leukemia.Therefore, targeting mitochondrial dynamic proteins is a strong candidate target for future treatments. Aims: We aimed to explore whether mitochondrial dynamics play a role in Arsenic Trioxide(ATO)-induced apoptosis in acute promyelocytic leukemia (APL) NB4 cells and its mechanism. Methods: (1) We cultured human APL NB4 cells. (2)The cells were treated with different concentration of ATO for 24/48 h, and the cell viability was measured by cell counting kit-8(CCK-8) to determine the IC50 of ATO.(3) The apoptotic rate was measured by Annexin V-FITC/PI double staining flow cytometry(FCM).the number of mitochondria was measured by Mitotracker Deep Red staining flow cytometry(FCM). (4) The mRNA expression levels of Drp1, Opa1, Mfn1, and Mfn2 are measured by real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). Results: We found that during ATO-induced apoptosis, the mitochondrial abundance of surviving cells increased, the mitochondrial division gene Drp1 was up-regulated, and the expression of mitochondrial fusion genes Opa1, Mfn1, and Mfn2 was down-regulated. Summary/Conclusion: Our results indicate that NB4 cells can survive during ATO treatment by regulating the number of mitochondria. It is mediated by regulating the expression of mitochondrial dynamic proteins Drp1, Opa1, Mfn1 and Mfn2.
Facts and Hopes for Gut Microbiota Interventions in Cancer Immunotherapy
Authors: Diwakar Davar, Hassane M. Zarour
Journal: Clinical Cancer Research (2022)

Abstract Immune checkpoint inhibitors (ICI) targeting cytotoxic T-lymphocyte–associated antigen 4 (CTLA-4) and programmed death 1 (PD-1) proteins transformed the management of advanced cancers. Many tumor-intrinsic factors modulate immunological and clinical responses to such therapies, but ample evidence also implicates the gut microbiome in responses. The gut microbiome, comprising the bacteria, archaea, fungi, and viruses that live in the human digestive tract, is an established determinant of host immunity, but its impact on response to ICI therapy in mice and humans with cancer has only recently been appreciated. Therapeutic interventions to optimize microbiota composition to improve immunotherapy outcomes show promise in mice and humans with cancer. In this review, we discuss the rationale for gut microbiome–based cancer therapies, the results from early-phase clinical trials, and possible future developments.
PB1784: CYTOGENETIC LANDSCAPE OF 87 MOROCCAN ACUTE MYELOID LEUKEMIA PATIENTS
Authors: E. M. Mahtat, H. MOUSSA BOUH, S. JENNANE, M. ABABOU, S. AHCHOUCH, A. HAMMANI, H. EL MAAROUFI, H. EDDOU, K. DOGHMI
Journal: HemaSphere (2022)

Background: Cytogenetics and molecular biology are the most important diagnostic tools for determining prognosis in acute myeloid leukemia (AML). In our country, access to molecular biology tests is limited compared to conventionnal cytogenetics. Still data about cytogenetics in AML moroccan patients are scarce. Aims: Describe cytogenetic landscape of patients diagnosed with acute myeloid leukemia treated in our center. Methods: We conducted a descriptive retrospective analysis of 87 adult patients diagnosed with acute myeloid leukaemia treated in our unit. Results: 87 patients diagnosed with acute myeloid leukmemia were included. Median age was 45 years and 25% of patients were older than 60 years. The cytogenetic analysis failed in 13 patients (15%). Prognostic group distribution according to the ELN 2017 was found to be favorable in 17 patients (19,5%) with 6 cases of t (8; 21) and 7 cases of inv (16), 4 patients had t(15;17). Intermediate group was predominant (48,5%), 28 patients (32%) had normal karyotype.15 patients (17%) had unfavourable cytogenetics including 9 patients with complex karytype and 3 patients with del7q. Intermediate risk cytogenetics were predominant in patients under 60 years old whereas Unfavourable karyotypes were more frequent in older patients. Summary/Conclusion: The landscape of karyotype abnormalities in our population found a predominance of intermediate risk group enhancing the need of molecular biology to refine prognosis and guide the treatment of AML patients in Morocco.