AB1143 MIR-10B EXPRESSION IN PSORIATIC ARTHRITIS PATIENTS WITH DAPSA SCORE REMISSION OR LOW DISEASE ACTIVITY
Authors: Ş. S. Köksal, Z. Öztürk, U. Alkurt, D. Karatas, G. Güney Eskİler, E. Çokluk, A. Deveci Ozkan, Ü. Erkorkmaz, E. Gönüllü
Journal: Annals of the Rheumatic Diseases (2023)

<h3>Background</h3> Psoriatic arthritis (PsA) is a heterogeneous inflammatory rheumatologic disease associated with psoriasis. The etiopathogenesis of PsA has not been fully elucidated. Although activity scores are used in the follow-up of patients, reliable biomarkers are not yet available. MicroRNAs (miRNA) are non-coding RNA oligonucleotides whose cellular expression levels change in inflammatory and autoimmune diseases and provide gene expression regulation. miRNAs are being investigated for their potential biomarker properties in the diagnosis and follow-up of psoriatic arthritis. <h3>Objectives</h3> In this context, the current study aimed to determine the changes in mir-10b expression level in patients with PsA who have remission/low disease activity according to DAPSA score and in age-sex matched healthy population. <h3>Methods</h3> Ethics committee approval was obtained from Sakarya University Ethics Committee for the study (E-71522473-050.01.04-15102135). RNA isolation, cDNA synthesis and RT-PCR analysis were performed in 30 PsA patients (19 Female and 11 Males) and age/sex matched control group (20 Females and 11 Males) with DAPSA scores in remission or low disease activity, who applied to Sakarya University Internal Medicine Rheumatology Clinic between January 2019 and February 21, miR-10b expression levels. U6 was used as internal control. IBM SPSS Statistics 26 program was used for statistical analysis. <h3>Results</h3> The mean age of the patients with psoriatic arthritis was 47.4±13.4, and the control group was 46.6±12.9 (p=0.78). No gender difference was analyzed between the two groups. (p=0.92).In addition, it was analyzed that the miR-10b expression level was 0.90-fold in PsA patients compared to the control group, and the expression level did not change significantly compared to the control group (p=0.53). <h3>Conclusion</h3> In our study, no statistically significant difference was observed in terms of miR-10b expression in PsA patients with DAPSA remission-low disease activity when compared to healthy controls. Further studies are needed in patients with moderate and high activity psoriatic arthritis. *The study was supported by the 2209-A Tubitak Student Project (1919B012101025). <h3>References</h3> [1]Rida, Mohamad Ali, and Vinod Chandran. "Challenges in the clinical diagnosis of psoriatic arthritis." <i>Clinical Immunology</i> 214 (2020): 108390. [2]Veale, Douglas J., and Ursula Fearon. "The pathogenesis of psoriatic arthritis." <i>The Lancet</i> 391.10136 (2018): 2273-2284. [3]Nakasa, Tomoyuki, et al. "A mini-review: microRNA in arthritis." Physiological Genomics 43.10 (2011): 566-570. <h3>Acknowledgements:</h3> NIL. <h3>Disclosure of Interests</h3> Şevval Sultan Köksal Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Zeynep Öztürk Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Umut ALKURT Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Damla Karatas Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Gamze GÜNEY ESKİLER Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Erdem ÇOKLUK Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Asuman Deveci Ozkan Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Ünal ERKORKMAZ Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025)., Emel Gönüllü Grant/research support from: The study was supported by the 2209-A Tubitak(Scientific and Technological Research Council of Turkey) Student Project (1919B012101025).