PB1796: DETERMINATION OF THE RELATIONSHIP BETWEEN NEXT GENERATION SEQUENCING AND FLOW CYTOMETRY RESULTS IN NEWLY DIAGNOSED ACUTE MYELOBLASTIC LEUKEMIA
Authors: I. Yavasoglu, A. H. E. küçükdiler, I. erdoğdu, Z. bolaman
Journal: HemaSphere (2022)

Background: Flow cytometry is one of the classical diagnostic tools used in the diagnosis of AML. In particular, the use of these two methods is essential for minimally measurable disease. According to our current knowledge, there is only one study in the literature that evaluated the expression of surface markers detected by both NGS and flow cytometry. In this study, NPM1, FLT3 (TKD and ITD), KRAS and NRAS signal mutations were found to be associated with high CD33 levels Aims: In this study, we aimed to determine the relationship between flow cytometrically determined surface markers in newly diagnosed AML patients whose genetic profile was determined by the NGS method Methods: Atotal of 120 patients diagnosed with AML by histochemical methods and flow cytometric analysis were evaluated retrospectively. Flow cytometric examination was performed using Beckman Coulter Navious 3L10C device (10 colors). NGS was performed with bone marrow aspiration samples taken at the time of diagnosis by the Molecular Pathology unit. Sequence analysis was performed using the Illumina MiniSeq next-generation sequencing system and supporting automated platforms and the QIAseq Targeted Myeloid Neoplasms DNA panel (DHS-003Z) containing 141 genes and 5887 primers for testing. The obtained raw data were first transferred to the QCI-A software where variants would be examined for bioinformatics analysis in the same laboratory. Variants transferred to QCI-Interpret, phenotype-related molecular hemato-pathological findings determined after bioinformatics interpretation were reported. The conformity of the quantitative data to the normal distribution was tested with the Shapiro-Wilk test and graphical examinations. Independent groups t-test was used for comparisons between two groups of normally distributed quantitative variables, and Mann-Whitney U test was used for comparisons between two groups of non-normally distributed quantitative variables. Statistical significance was considered as p<0.05. Results: ASXL1, ANKRD26, ATRX, BCR, BLM, BRCA1, BRCA2, CEBPA, CTCF, DNMT1, FLT3, KMT2A, KRAS, MSH6, NSD1, PTEN, RUNX1, SRSF2, SUZ12, TET2, U2AF1 in bone marrow aspiration samples and a significant correlation was found between WRN and high values of surface markers detected by flow cytometry. The some results are shown in Table 1. Image:Summary/Conclusion: The effects of CD 36 and 9 on platelet functions and cell signal interactions are well known. In this context, the relationship between the positivity of both surface markers and the ANKRD26, ATRX, KMT2A, MSH6, NSD1, RUNX1 and SUZ12 mutations is important. The correlation of CD 7, which is aberrantly expressed surface markers in AML, with CEBPA and KRAS was also remarkable. However, a significant correlation was found between high CD 33 values and BLM, TET2 and WRN positivity. These results are critical for future targeted therapies, and multi-center studies with larger numbers of patients are needed.