POS0099 THE CELLULAR METABOLISM OF SLE NK CELLS IS PRIMARILY ALTERED AT THE LEVEL OF MITOCHONDRIAL RESPIRATION.
Authors: N. Fluder, M. Humbel, F. Bellanger, A. Horisberger, C. Fenwick, C. Ribi, D. Comte
Journal: Annals of the Rheumatic Diseases (2022)

Background Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease, involving the development of autoreactive cells and autoantibodies. Natural Killer (NK) cells are innate immune cells that mediate the interaction between the innate and adaptive immune system, however their role in SLE is incompletely understood. SLE NK cells are decreased in peripheral blood, exhibit reduced cytotoxicity, and impaired cytokine production (1, 2). Furthermore, SLE NK cells present phenotypic alterations: increased expression of CD38 and altered upregulation of SLAMF7 after activation (3). To date, few studies evaluated the molecular mechanisms underlying NK cell dysfunction in SLE. Objectives We examined immunometabolic alterations of SLE NK cells. First, we characterized the cellular metabolism of SLE NK cells by assessing glycolysis and oxidative phosphorylation (OXPHOS) at basal level. Then, we evaluated how cellular metabolism can be manipulated to enhance NK cell function. In this perspective, we examined how the ligation of CD38 with daratumumab (DARA) and SLAMF7 with elotuzumab (ELO) modulate glycolysis and OXPHOS. Methods NK cells of cryopreserved PBMC from SLE patients were isolated. Glycolysis and OXPHOS were studied using XF e 96 Seahorse. Expression of metabolic receptors (CD71, GLUT-1, CD98), mitochondrial function (mitochondrial membrane potential, mass) and calcium influx were investigated by FACS. Mitochondrial structure was evaluated by electron and confocal microscopy. Results First, we examined the cellular metabolism of SLE NK cells compared to healthy cells. We observed that OXPHOS is significantly increased in SLE NK cells (Figure 1A), whereas glycolysis was normal (Figure 1B). Furthermore, the mitochondrial mass and membrane potential (by FACS (Figure 1C) and confocal microscopy) were increased in SLE. Electron microscope imaging showed profound alterations in SLE NK cell mitochondrial ultrastructure (Figure 1D). No significant differences in the expression of key metabolite transporters involved in mitochondrial fueling (CD71, GLUT-1, CD98) was observed in SLE NK cells compared to healthy controls. Second, we examined how ligation of DARA and ELO influences the metabolism of healthy NK cells. Our data showed that ELO primarily enhances NK cell OXPHOS (Figure 1E), whereas DARA mainly increases glycolysis. Consistently, ELO also increases mitochondrial membrane potential and expression of metabolite transporters CD71, GLUT-1 and CD98. Next, we examined the effect of DARA and ELO on SLE NK cells. While stimulation with DARA adequately increases glycolysis in SLE NK cells, engagement with ELO fails to properly increase OXPHOS (Figure 1F), expression of cell surface transporters, mitochondrial membrane potential and mass. Conclusion Our data suggest that SLE NK cells exhibit alterations in cellular metabolism, primarily involving mitochondrial respiration. In contrast, glucose metabolism is similar to that of healthy NK cells. Additionally, ELO and DARA mediate the activation of healthy NK cells through the engagement of different metabolic pathways: OXPHOS and glycolysis, respectively. Therefore, priming SLE NK cells with ELO is unable to adequately engage their dysfunctional mitochondrial respiration. These findings provide important insights on the alteration present in SLE NK cells and contribute to a better understanding of the pathogenesis of the disease. References [1]Spada R, Rojas JM, Barber DF. Recent findings on the role of natural killer cells in the pathogenesis of systemic lupus erythematosus. J Leukoc Biol. 2015;98(4):479-87. [2]Park Y-W, Kee S-J, Cho Y-N, Lee E-H, Lee H-Y, Kim E-M, et al. Impaired differentiation and cytotoxicity of natural killer cells in systemic lupus erythematosus. Arthritis &amp; Rheumatism. 2009;60(6):1753-63. [3]Humbel M, Bellanger F, Fluder N, Horisberger A, Suffiotti M, Fenwick C, et al. Restoration of NK Cell Cytotoxic Function With Elotuzumab and Daratumumab Promotes Elimination of Circulating Plasma Cells in Patients With SLE. Front Immunol. 2021;12:645478. Acknowledgements This study received funding from the Swiss National Science Foundation (Ambizione PZ00P3_173950 to DC). Disclosure of Interests None declared