tbuckley/cabot-search huggingface dataset:
SINGLE MAMMALIAN CELLS
Journal: JAMA (1960)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Induction chemotherapy, extrapleural pneumonectomy (EPP), and adjuvant hemithoracic radiation are feasible and effective for locally advanced malignant pleural mesothelioma (MPM)
Authors: R. M. Flores, L. Krug, K. E. Rosenzweig, A. Vincent, T. Akhurst, R. Heelan, V. W. Rusch
Journal: Journal of Clinical Oncology (2004)

7193
EPCO-05. LOSS OF THE H4 LYSINE METHYLTRANSFERASE KMT5B DRIVES INVASION / MIGRATION BY DEPLETING H3K27me3 AT LOCI OTHERWISE RETAINED IN H3K27M MUTANT DIPG CELLS
Authors: Ketty Kessler, Alan Mackay, Valeria Molinari, Haider Tari, Anna Burford, Andrea Sottoriva, Maria Vinci, Chris Jones
Journal: Neuro-Oncology (2020)

Abstract Diffuse intrinsic pontine glioma (DIPG) and other diffuse midline glioma (DMG) are characterised by K27M mutations in histone H3 variants. The major functional consequence is a global loss of the repressive mark H3K27me3, causing a raft of transcriptional changes promoting tumorigenesis, although certain key loci retain trimethylation, such as CDKN2A/B. We recently identified subclonal loss-of-function mutations in the H4 lysine methyltransferase KMT5B to be associated with an enhanced invasion/migration, but the mechanism by which this occurred was unclear. Here we show by ChIP-seq using patient-derived subclonal DIPG models and CRISPR-Cas9 depletion that loss of KMT5B (or KMT5C) causes a paradoxical increase in global levels of H4K20me3 in promoters and regulatory regions, only ablated by knocking out both enzymes. Loss of KMT5B alone further causes loss of the majority of otherwise retained H3K27me3 loci in DIPG cells, although CDKN2A/B itself was spared. De-repression occurred at bivalent loci marked by H3K4me3 and had elevated gene expression by RNAseq; these were significantly enriched for genes involved in chromatin remodelling and invasion/migration, the latter including MMP9/MMP24. Phenotypic assessment of the models in vitro by high-throughput imaging demonstrated significantly increased invasion and migration in association with either KMT5B or KMT5C loss, but not both. Quantitative proteomic assessment of the secretome identified factors by which a minority of KMT5B-deficient cells may signal to promote motility of the neighbouring populations. These data suggest a previously unrecognised trans-histone (H4/H3) interaction in DIPG cells with a potentially profound effect on their diffusely infiltrating phenotype.
Registry of the X-Linked Lymphoproliferative Syndrome
Journal: JNCI Journal of the National Cancer Institute (1987)

Registry of the X-Linked Lymphoproliferative Syndrome Get access JNCI: Journal of the National Cancer Institute, Volume 79, Issue 4, October 1987, Page 909, https://doi.org/10.1093/jnci/79.4.909-a Published: 01 October 1987
Words − sentences = ?
Authors: Lee Osterhout, Michael Bersick
Journal: Behavioral and Brain Sciences (1999)

Pulverm&amp;uuml;ller advocates an experimental design in which well-matched tokens from various word categories are presented in list form to subjects making rapid lexical decisions about these stimuli. Implicit in this paradigm are several problematic assumptions. We conclude that such experiments are not likely to tell us much about the biological bases of human language.
<i>QuickStats:</i>Percentages* of Residential Care Communities and Adult Day Services Centers That Provided<sup>†</sup>Selected Services — United States, 2014
Journal: MMWR Morbidity and Mortality Weekly Report (2016)

In 2014, a greater percentage of residential care communities than adult day service centers provided five of seven selected services. The majority of residential care communities provided pharmacy services (82%); followed by transportation for social activities (79%); physical, occupational, or speech therapy (69%); hospice (62%); skilled nursing (59%); and mental health services (52%). Fewer than half provided social work services (48%). The majority of adult day services centers provided transportation for social activities (69%); skilled nursing (66%); and social work (52%). %). Fewer than half provided physical, occupational, or speech therapy (49%). One third or less provided mental health (33%), pharmacy (27%), and hospice services (12%).
Sequential chemoradiotherapy (SCRT) for larynx preservation (LP): Results of the randomized phase II TREMPLIN study.
Authors: J. Lefebvre, Y. Pointreau, F. Rolland, M. Alfonsi, A. Baudoux, C. Sire, D. De Raucourt, E. Bardet, C. Tuchais, P. Garaud, G. Calais
Journal: Journal of Clinical Oncology (2011)

5501 Background: Induction chemotherapy (ICT) followed by concurrent chemoradiotherapy or by concurrent bioradiotherapy (BRT) is under evaluation in locally advanced head and neck squamous cell carcinoma (SCC). The TREMPLIN study assesses SCRT specifically for LP. Methods: Previously untreated patients (pts) with stage III-IV larynx/hypopharynx SCC and candidates for total laryngectomy were eligible. Pts received 3 ICT cycles (docetaxel and cisplatin both 75 mg/m² day 1 and 5-FU 750 mg/m²/day on days 1-5). Pts responding < 50% had surgery. In case of response > 50 %, pts were randomized to arm A: RT (70 Gy) with cisplatin (100 mg/m² on days 1, 22 and 43 of RT) or arm B: RT (70 Gy) with cetuximab (400 mg/m² loading dose before RT and 250 mg/m² on the first day of the 7 weeks of RT). Primary endpoint, LP 3 months after treatment, did not differ between arms but with better compliance to BRT, secondary endpoints included larynx function preservation (LFP) at 18 months after treatment, survival, feasibility of salvage surgery and tolerance to treatment. Results: 153 pts were enrolled; 116 pts could be randomized after ICT (60 in arm A, 56 in arm B). In intent to treat evaluation, 52 pts (86 %) in arm A and 46 pts (82 %) in arm B had LFP 18 months after treatment or at death (p= .41). Overall survival was respectively 85 % and 86 %. With a mean follow-up of 32 months local failures occurred in 5 pts in arm A (none were salvaged) and in 12 pts in arm B (7 were salvaged) for a similar ultimate local failure rate of 5 pts in each arm. Late toxicity (any grade) occurred more often in arm A (p= 0.111). Conclusions: LFP and survival were similar in both arms, acute and late toxicity were more common in arm A. Local failures occurred more often in arm B, but salvage surgery was only feasible in this arm resulting in similar ultimate local control. Additional research on LP is warranted.
Oral Presentations, Monday, 22 September 2009
Authors: C Salazar Ramirez, I Bellido Este ´vez, M De La Torre-Prados, Z Wang, J Poulsen, K Møller, H Kehlet, A Perner, L Papazian, J.-M Forel, A Gacouin, G Perrin, S Jaber, J.-M Arnal, D Perez, J.-M Seghboyan, J.-M Constantin, P Courant, A Roch, A Larentzakis, H Markogiannakis, P Drimousis, A Xanthaki, M Toutouza, E Samara, N Pachylaki, E.-M Theodoraki, D Theodorou, K Toutouzas, S Katsaragakis, T Mikula, W Stan ´czak, J Cianciara, A Wiercin ´ska-Drapało
Journal: Intensive Care Medicine (2009)

INTRODUCTION.To determine the effectiveness of an educational program based on Surviving Sepsis Campaign guidelines to reduce sepsis mortality in several hospitals in Spain.METHODS.Eleven Spanish ICUs, in the South of Spain (Andalucia) were included in a prospective study.A multicentric study realised during 2005 and 2006.We determined the rate of compliance of the resuscitation bundle (first 6 h) and the management bundle (first 24 h) during 2 months before the educational program and 4 months after the educational program.RESULTS.Three hundred and thirty patients were enrolled in this analysis: severe sepsis (29.4%) or septic shock (70.6%) admitted in intensive care units.There were no significant differences between the groups with respect to base-line characteristics and severity of illness measured by the Acute Physiology and Chronic Health Evaluation II were similar for both groups.Patients characteristics were as follows: age, 62 (60, 63) years; APACHE II score, 21 (20, 22).The main sources of infection were: lung (36.1%), abdomen (28.1%) and UTI (11%).CVP and Svc02 monitoring, antibiotics, fluid resuscitation, mechanical ventilation, vasopressors, inotropes, corticosteroides, and rhAPC were included in the educational program.The patients in the after EP period had measured more Svc02 (36.2 vs. 15.5%),CVP (45.7 vs. 39.9%) and lactate (27.7 vs. 23.2%)All patients received broad-spectrum antibiotics, patient in the after group received more corticosteroids (35 vs. 25%), a lower lactate (3.8 vs. 5.19 mmol/l), blood culture (47.4 vs. 37.5%), were more likely to receive intravenous fluids of [20 ml/kg body weight before vasopressor administration (38 vs. 36%), patient in the after group were less likely to require vasopressor (43 vs. 47%).In the after EP period these patients had a shorter length of stay in intensive care (238 vs. 280 h, we reduced the length in 15.1%) and shorter hospital length of stay (688 vs. 719 h).Mortality in ICU pre-EP was 43.8% and after-EP was 31.6%.The percentage of compliance with 6-h and 24-h bundle in the after-EP increased, respectively (9.85%, P \ 0.01 and 8.86%), total compliance was also 46.62%. CONCLUSIONS.Our study found the implementation of a standardized order set for the management of septic shock was associated with an important decreased of the mortality (12.2%), improving early and accurate diagnosis and increasing the use of appropriate treatment and interventions.
Book Listings
Journal: Annals of Internal Medicine (2001)

Book Listings16 October 2001Book ListingsSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-135-8_Part_1-200110160-00001 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail CardiologyAcute Myocardial Infarction and Other Acute Ischemic Syndromes2nd edition. RM Califf, ed. 232 pages. Philadelphia: Current Medicine; 2001. $125.00. ISBN 1573401595. Order phone 215-574-2266.The Adult with Tetralogy of FallotMA Gatzoulis and DJ Murphy Jr, eds. 95 pages. Armonk, NY: Futura; 2001. $30.00. ISBN 0879934905. Order phone 800-877-8761.Atlas of Atherosclerosis: Risk Factors and Treatment2nd edition. PW Wilson, ed. 269 pages. Philadelphia: Current Medicine; 2000. $125.00. ISBN 1573401374. Order phone 215-574-2266.Basic Dysrhythmias: Interpretation and Management3rd edition. RJ Huszar. 544 pages. St. Louis: Mosby-Year Book; 2002. $37.00. ISBN 0323012442. Order phone 314-872-8370.Cardiology Secrets2nd edition. OV Adair, ed. 343 ... Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 16 October 2001Volume 135, Issue 8_Part_1Page: B1KeywordsComputed axial tomographyInterleukinsPrevention, policy, and public health ePublished: 16 October 2001 Issue Published: 16 October 2001 Copyright & PermissionsCopyright © 2001 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Arteriovenous shunts for prolonged intermittent hemodialysis. Technique, survival, and complications in 57 patients
Authors: L. H. Wolin
Journal: JAMA (1967)

To facilitate repeated intermittent hemodialysis, 57 patients at the University of Colorado Medical Center and the Denver Veterans Administration Hospital had arteriovenous shunts placed into their arms. The radial artery and cephalic vein were cannulated 3 cm proximal to the wrist on the nondominant arm. Fifty-four of the 57 patients had both vessels cannulated through a single incision, with an average length of 5 cm. The ligating suture on the vessel was tied around that portion of the siliconized rubber (Silastic) tube containing the vessel tip. Disengagement at this junction or postoperative bleeding requiring transfusion or surgical intervention never occurred. Twelve of the 17 patients who experienced complications (clotting, 12 instances; infection, 7; spasm, 3; disengagement, 2; and erosion, 2) required revision of their shunts. No complication was ever observed in the remaining 40 patients during their entire stay on the dialysis program.
Results of retransplantation for recurrent hepatitis C
Authors: S Roayaie
Journal: Hepatology (2003)

Retransplantation for recurrent hepatitis C virus (HCV) has been evaluated in small series. In this study, patients undergoing transplantation for HCV-related cirrhosis with subsequent retransplantation more than 90 days for recurrent HCV (proven by pathologic examination of the explant and exclusion of other factors) were prospectively followed. This group was compared with a simultaneous cohort without HCV infection undergoing retransplantation more than 90 days after primary transplantation. Forty-two patients underwent retransplantation for recurrent HCV with a median survival of 12.9 ± 6.7 months after retransplantation. Twenty patients (48%) were dead at 6 months, and 13 (65%) of these deaths were due to sepsis. On univariate analysis, creatinine level greater than or equal to 3 mg/dL, platelet count less than 100,000/μL, prothrombin time (PT) greater than or equal to 16 seconds, alkaline phosphatase level less than or equal to 240 U/L, γ-glutamyltransferase level less than or equal to 130 U/L, and donor age of 60 years or greater all correlated significantly with shorter survival after retransplantation. PT and donor age were predictors of survival on multivariate analysis. Patients undergoing retransplantation for recurrent HCV had a significantly shorter median survival than the 55 patients undergoing retransplantation for other chronic reasons of graft loss (75.6 ± 17.7 months). In conclusion, median survival after liver retransplantation for recurrent HCV is significantly shorter than after retransplantation for other causes of late graft loss. Most deaths occur in the first 6 months and are due to sepsis. Candidates for retransplantation with a preoperative PT less than 16 seconds and those receiving grafts from donors younger than 60 years can expect a significantly longer median survival after retransplantation. (Hepatology 2003;38:1428-1436.)
A Randomized, Controlled Trial With 6-Month Follow-Up of Repetitive Transcranial Magnetic Stimulation and Electroconvulsive Therapy for Severe Depression
Authors: Savitha Eranti
Journal: American Journal of Psychiatry (2007)

Objective: Repetitive transcranial magnetic stimulation (rTMS) has been reported to be as effective as electroconvulsive therapy (ECT) for major depression. The authors conducted a multicenter randomized, controlled trial to test the equivalence of rTMS with ECT. Method: Forty-six patients with major depression referred for ECT were randomly assigned to either a 15-day course of rTMS of the left dorsolateral prefrontal cortex (N=24) or a standard course of ECT (N=22). The primary outcome measures were the score on the 17-item Hamilton Depression Rating Scale (HAM-D) and the proportion of patients with remissions (Hamilton score, ≤8) at the end of treatment. Secondary outcomes included mood self-ratings on the Beck Depression Inventory-II and visual analogue mood scales, Brief Psychiatric Rating Scale (BPRS) score, and both self-reported and observer-rated cognitive changes. The patients were followed up after 6 months. Results: HAM-D scores at the end of treatment were significantly lower for ECT, with 13 patients (59.1%) achieving remission in the ECT group and four (16.7%) in the rTMS group. However, at 6 months the HAM-D scores did not differ between groups. Beck scale, visual analogue mood scale, and BPRS scores were lower for ECT at the end of treatment and remained lower after 6 months. Self- and observer-rated cognitive measures were similar in the two groups. Conclusions: rTMS was not as effective as ECT, and ECT was substantially more effective for the short-term treatment of depression.
Increased expression of matrix metalloproteinase-II in experimental liver fibrosis in rats
Authors: T TAKAHARA
Journal: Hepatology (1995)

Matrix metalloproteinase-II (MMP-II, 72-kd type IV collagenase, or gelatinase) is one of the gene families of zinc enzymes capable of degrading extracellular matrix molecules, and specifically of degrading type IV and V collagens, gelatin, fibronectin, and elastin. In this study, we used both the liver fibrosis model and the reversibility model of experimental cirrhosis to clarify how MMP-II participates in liver fibrosis of rats. To produce fibrosis model, rats received subcutaneous injections of CCI4 twice weekly for 7, 9, or 14 weeks. For the reversibility model, rats were treated with CCl4 three a week for 8 weeks and killed at 3, 7, 14, 28, or 42 days after discontinuation of treatment. MMP-II gene expression was studied by Northern hybridization technique, and gelatinase activity of MMP-II was examined by zymography using gelatin substrate. At the same time, an immunohistochemical study using anti-type IV collagen antibody was carried out. In liver fibrosis model, nodule formation was established at 14 weeks. Immunodeposit of type IV collagen was increased in wide fibrous septa and was clearly observed along sinusoidal wall. Gene expression of MMP-II increased up to 7 to 12 times compared with that of controls, with the expression rate being maximum at an intermediate stage of fibrosis. Zymography showed the expressions of both 65-kd latent MMP-II, which is confirmed to be activated by adding p-aminophenylmercuric acetate, and 62-kd active MMP-II during fibrosis. The expression of both forms increased 13 to 28 times as the fibrosis progressed. By contrast, little latent MMP-II was detected in control livers. The percent active form to total MMP-II at each stage was elevated most at an intermediate stage of fibrosis up to 30% and decreased to 16% in the cirrhotic stage. As cirrhosis reversed, fibrous septa became thin but still persisted at 42 days in the reversibility model. Immunostaining of type IV collagen was increased in thin septa and faintly observed along sinusoid. Gene expression was elevated 18-fold and recovered gradually to remain elevated 18-fold and recovered gradually to remain elevated at 42 days after the discontinuation of intoxication. Expressions of both active and latent forms detected by zymography were elevated 15-fold during the early reversible stage and decreased gradually after the discontinuation of intoxication. These results indicated MMP-II may participate in pathogenesis of liver fibrosis and cirrhosis.
Asbestos Exposure, Pleural Mesothelioma, and Serum Osteopontin Levels
Authors: Harvey I. Pass, Dan Lott, Fulvio Lonardo, Michael Harbut, Zhandong Liu, Naimei Tang, Michele Carbone, Craig Webb, Anil Wali
Journal: New England Journal of Medicine (2005)

We investigated the presence of osteopontin in pleural mesothelioma and determined serum osteopontin levels in three populations: subjects without cancer who were exposed to asbestos, subjects without cancer who were not exposed to asbestos, and patients with pleural mesothelioma who were exposed to asbestos.A group of 69 subjects with asbestos-related nonmalignant pulmonary disease were compared with 45 subjects without exposure to asbestos and 76 patients with surgically staged pleural mesothelioma. Tumor tissue was examined for osteopontin by immunohistochemical analysis, and serum osteopontin levels were measured by an enzyme-linked immunosorbent assay.There were no significant differences in mean (+/-SE) serum osteopontin levels between age-matched subjects with exposure to asbestos and subjects without exposure to asbestos (30+/-3 ng per milliliter and 20+/-4 ng per milliliter, respectively; P=0.06). In the group with exposure to asbestos, elevated serum osteopontin levels were associated with pulmonary plaques and fibrosis (56+/-13 ng per milliliter) but not with normal radiographic findings (21+/-5 ng per milliliter), plaques alone (23+/-3 ng per milliliter), or fibrosis alone (32+/-7 ng per milliliter) (P=0.004). Serum osteopontin levels were significantly higher in the group with pleural mesothelioma than in the group with exposure to asbestos (133+/-10 ng per milliliter vs. 30+/-3 ng per milliliter, P<0.001). Immunohistochemical analysis revealed osteopontin staining of the tumor cells in 36 of 38 samples of pleural mesothelioma. An analysis of serum osteopontin levels comparing the receiver-operating-characteristic curve in the group exposed to asbestos with that of the group with mesothelioma had a sensitivity of 77.6 percent and a specificity of 85.5 percent at a cutoff value of 48.3 ng of osteopontin per milliliter. Subgroup analysis comparing patients with stage I mesothelioma with subjects with exposure to asbestos revealed a sensitivity of 84.6 percent and a specificity of 88.4 percent at a cutoff value of 62.4 ng of osteopontin per milliliter.Serum osteopontin levels can be used to distinguish persons with exposure to asbestos who do not have cancer from those with exposure to asbestos who have pleural mesothelioma.
针对白癜风试验结局的国际行动计划 (INFO)
Authors: V. Eleftheriadou, I. Hamzavi, A.G. Pandya, P. Grimes, J.E. Harris, R.H. Huggins, H.W. Lim, N. Elbuluk, B. Bhatia, A. Tovar‐Garza, A.F. Nahhas, T. Braunberger, K. Ezzedine
Journal: British Journal of Dermatology (2019)

为了开发和测试一种治疗, 研究者们会测量一个“结局”。例如, 这一“结局”可能包括测量白癜风中一块白色斑片的颜色恢复(复色)速度。白癜风是一种皮肤病, 会导致皮肤和(有时)毛发变白。目前尚无治愈手段, 也没有针对白癜风治疗的确凿临床推荐意见。这一现象背后的主要原因之一是不同研究测量不同的结局; 因此这些研究不能被整合和比较。白癜风结局国际行动计划(白癜风 INFO)包括来自全世界超过 24 个国家的患者、他们的照护者、医学期刊编辑和医生, 近期, 该研究就应在每项试验中测量的一套白癜风结局达成了一致:即皮损复色、治疗副作用/危害和白癜风皮损复色后颜色能保持多久。但对于应使用何种量表(结局指标)测量复色方面, 仍未在患者和医生中达成一致。这意味着, 即使未来的所有试验都测量复色, 但使用不同的量表的话, 研究结果仍不能被整合和比较。实施这项国际研究的目的是从患者的角度定义成功复色, 和提出如何以及何时在白癜风临床试验中评估复色。作者们与白癜风患者以及他们的父母/照护者们召开了 3 次研讨会。共招募了 73 名参与者。患者角度的“成功复色”意味着白癜风斑片达到了超过 80% 复色。应在每项试验中测量复色的百分比四分位数 (0‐25%, 26‐50%, 51‐79%, 80‐100%) 和患者角度结果的美容可接受性。总之, 这是最大规模的患者结局研讨会。研究者就使用相同量表在未来试验中测量复色达成一致将带来更丰富的数据和因此更清晰的临床推荐意见。
Increasing US mortality from infectious diseases
Authors: B. Randall
Journal: JAMA (1996)

<h3>In Reply.</h3> —Somatization is a multidetermined phenomenon and represents the product of psychological, behavioral, interpersonal, and social factors. Therefore, effective treatment is often complex and multimodal. While the primary care physician is undoubtedly the cornerstone of therapy, he or she alone may not be sufficient. Primary care physicians can care more effectively for somatizing patients with the assistance of management guidelines, psychiatric consultation, and specific training and educational programs, as Smith et al<sup>1</sup>have shown using psychiatric consultation letters. However, more definitive symptom relief and improved functional status may require a more intensive targeted effort, which requires the time and expertise the primary care physician lacks. Contrary to Drs Fabian, Langsjoen, and Kent, we find impressive evidence of the efficacy and effectiveness of cognitive and behavioral techniques in a variety of conditions, including isolated functional somatic symptoms, somatization disorder, and hypochondriasis.<sup>2 6</sup>Although it is true that nonspecific