tbuckley/cabot-search huggingface dataset:
An miRNA assay for the classification of benign and neoplastic lesions in pancreatic fine-needle aspirates.
Authors: E. Labourier, M. Lloyd, B. Andruss, A. Adai, A. Schwarzbach
Journal: Journal of Clinical Oncology (2011)

163 Background: Improved diagnosis of pancreatic ductal adenocarcinoma (PDAC) versus benign pancreatic lesions is an urgent clinical need. We previously reported a laboratory-developed test based on the differential expression of two miRNAs, miR-196a and miR-217, with a sensitivity and specificity of 95% in formalin-fixed, paraffin-embedded (FFPE) specimens with ≥60% tumor content. Here, we report the development and validation of an miRNA-based research test optimized for low % tumor specimens and fine needle aspirates (FNA). Methods: RNA was extracted using methods developed and validated by the Asuragen Services Laboratory from frozen, FFPE or FNA pancreatic specimens. One to three endoscopic ultrasonography-guided FNAs were collected, stored, and shipped in RNARetain, an RNA stabilization solution formulated in 1 mL single-use vials. Expression levels of up to 12 miRNAs were interrogated using TaqMan qRT-PCR assays. Results: A set of 211 specimens, including 94 PDAC, 96 chronic pancreatitis, and 21 normal, was used to develop a miRNA classifier to discriminate benign and low tumor content PDAC. Training on 95 FFPE specimens showed that expression analysis of miR-196a, -210, and -375 improved by 2- to 3-fold the detection rate of PDAC in specimens with 5-88% tumor. The classifier performance was further verified on 4 independent sets of specimens (n=116): frozen with high tumor content (80-100%), FFPE with high tumor content (60-95%), FFPE with low tumor content (10-60%), and FNA. The signature is now being evaluated in a multisite prospective study using FNAs collected in RNARetain; 110 specimens have been analyzed to date and we will report the relative performance of the 2- and 3-miRNA classifiers. Conclusions: Benign lesions and PDAC can be accurately classified based on the expression level of a few miRNAs in specimens with 5-100% tumor and in FNA. Testing of preoperative FNAs collected in RNARetain as part of routine clinical evaluation could in the future improve the differential diagnosis of PDAC and potentially reduce the rate of indeterminate or false-negative calls. [Table: see text]
Chekhov's Doctors
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
KIT with D816 Mutations Cooperates with CBFB-MYH11 for Leukemogenesis in Mice
Authors: Ling Zhao, J. Joseph Melenhorst, Lemlem Alemu, Stacie Anderson, Martha Kirby, Shelley Hoogstraten-Miller, Lauren Brinster, Maggie Kench, Yasuhiko Kamikubo, D. Gary Gilliland, Pu Paul Liu
Journal: Blood (2011)
FRIEDRICH VON ESMARCH—MILITARY SURGEON
Journal: JAMA (1963)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Complement C4 protein gene expression by rat hepatic lipocytes . Saint Louis University Health Sciences Center and St. Louis VA Medical Center, St. Louis, MO, and Alton Ochsner Medical Foundation, New Orleans, LA
Journal: Hepatology (1995)

The effect of continuous intraperitoneal infusion of undine diphosphoglucose on ethanlol-induced suppression of lipocyte proliferation was studied in regenerating rat livers from 1–4 days after hepatectomy. Proliferating lipocytes were positively identified using a two-sequence immunohistochemical staining for cytoplasmic desmin and bromodeoxyuridine-labelled nuclei. Hepatectomy rapidly stimulated lipocyte proliferation which peaked 2 days after hepatectomy (labelling index, 17.9 ± 0.81%). uridine diphosphoglucose or glucose infusion did not modify the time course of lipocyte proliferation. Ethanol feeding to hepatectomized rats receiving saline or glucose infusion resulted in a 71% (p < 0.005) and 61% (p < 0.005) inhibition of lipocyte proliferation, respectively, 2 days after hepatectomy, thereby abolishing the characteristic proliferative peak observed in rats not treated with ethanol. In contrast, uridine diphosphoglucose infusion doubled the labelling index (13.1 ± 2.34%) in ethanol-fed rats compared to that in corresponding rats treated with saline (5.28 ± 1.29%; p < 0.005) or glucose (6.51 ± 0.64%; p < 0.005). This resulted in the appearance of a proliferative peak, albeit smaller than normal, 2 days after hepatectomy. In sham-operated rats, lipocyte proliferation was low with a labelling index of 1.88 ± 0.13% at the time of operation and of 1.69 ± 0.23% 2 days thereafter. Uridine diphosphoglucose infusion to sham-operated rats for 2 days did not significantly affect lipocyte proliferation (labelling index 1.79 ± 0.06%). The present study demonstrated that uridine diphosphoglucose does not affect lipocyte proliferation in the regenerating or sham-operated livers, but that it partially reverses the ethanol-induced suppression of lipocyte proliferation after hepatectomy.
Oxidative metabolism in a rat hepatoma (N13) and isolated rat hepatocytes: A flow cytometric comparative study
Authors: G Juan
Journal: Hepatology (1996)

Recently, we have developed a new and fast kinetic method for assessing mitochondrial membrane potential by flow cytometry, based on the quantitation of the initial rate of rhodamine 123 (Rh123) uptake by living cells. This test has proved suitable to detect metabolic and toxic effects on mitochondria. To characterize energy metabolism in a rat hepatoma cell line (N13), we applied this method to assess several metabolic pathways that eventually generate mitochondrial membrane potential. Using this approach, we found that N13 hepatoma cells retain an oxidative capacity comparable with that observed in isolated hepatocytes under the same conditions. These results show that this cell line may represent an adequate biological model to perform metabolic and toxicological studies in vitro.
Esophagomyotomy Effective in Diffuse Spasm of Esophagus
Journal: JAMA (1965)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Medicaid Eligibility, Prenatal Care, and the Outcome of Pregnancy
Authors: David P. Page
Journal: JAMA (1991)

<h3>To the Editor. —</h3> The study by Piper et al<sup>1</sup>was interesting and timely. The authors provide data to suggest that providing eligibility for public insurance is not sufficient to affect health. I agree with the sentiments of Guyer's editorial<sup>2</sup>that the maternity care system itself must be addressed, not only insurance coverage. Still, I would like to make two points. First, the abstract by Piper et al states that "there were no concomitant improvements in... neonatal mortality." The data presented show a reduction in mortality from 5.6 per 1000 to 5.1 per 1000, an 8.9% reduction. Certainly this reduction could have been due to chance; the statistical analysis of the data does not allow one to conclude with 95% certainty that the difference was not due to chance. However, the observed improvement in neonatal mortality was significant to the 90% level of confidence, and so statistical analysis
Perceived versus measured functional vaginal capacity in cancer patients with sexual function concerns.
Authors: Vanessa Amy Kennedy, Stacy Tessler Lindau, Seiko Diane Yamada, Kristen Wroblewski, Emily Abramsohn, Jennifer Makelarski
Journal: Journal of Clinical Oncology (2013)

170 Background: Actual and perceived loss of vaginal capacity can be a source of distress among female cancer survivors. The objective of this study was to assess perceived (PC) versus measured (MC) functional vaginal capacity in patients presenting with sexual function concerns. Methods: This was a cross-sectional registry-based study of women seen at the Program in Integrative Sexual Medicine for Women and Girls with Cancer (PRISM) Clinic. During the visit, patients were presented with graduated vaginal dilators and asked to select the largest dilator they perceived could be inserted without pain (PC) and the dilator representing their desired functional capacity (DC) (for patients with a male partner, this was the size closest to the partner’s erect penis). Two models of dilators were offered. Dilators were numbered 1-24 in order of increasing volume. If the patient could accommodate the dilator chosen as PC without pain, she was examined with dilators of gradually increasing size until the patient reported discomfort. The largest dilator tolerated without pain was MC. Differences between PC and MC, and between DC and MC were calculated. The association between penetrative sexual activity in the prior 4 weeks and accuracy of PC was assessed using the Mann-Whitney U test. Results: Mean patient age was 46 years (range 21-80, N=69). Most patients had breast (43%) or a gynecologic cancer (16%); 29% had benign disease. Nearly half reported two or more sexual concerns; painful intercourse (81%), vaginal complaints (21%), and loss of libido (19%) were most common. Median PC was 50cm 3 (IQR 35-65cm 3 ), median MC was 81cm 3 (IQR 60-90cm 3 ), and median DC was 90cm 3 (IQR 81-132cm 3 ). PC equaled MC in 22%. PC was less than MC in 75% and less than DC in 81% of patients. Of patients with PC less than DC, 41% had MC equal to or larger than DC. PC was closer to MC in patients reporting penetrative sexual activity in the prior 4 weeks (p=0.02). Conclusions: In this single-site study, many cancer survivors seeking care for sexual concerns underestimate their functional vaginal capacity. Further study is needed to determine whether correcting patient perception of capacity lessens distress and improves function.
Reference Committees
Journal: JAMA (1968)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Metabolism and Pharmacokinetics of Alternate Drinking Water Disinfectants
Authors: Mohamed S. Abdel-Rahman, Daniel Couri, Richard J. Bull
Journal: Environmental Health Perspectives (1982)

The chlorination of surface waters is known to elevate trihalomethanes; consequently, chlorine dioxide (ClO2) is being considered as an alternative disinfectant. The primary products resulting from ClO2 disinfection of waters are chlorites (ClO2-) and chlorates (ClO3-). Studies in rats revealed that ClO2 is converted to chloride (Cl-), ClO2- and ClO3-. ClO2- and ClO3- are excreted as Cl-, ClO2- and Cl-, ClO2-, ClO3-, respectively. Radioactivity was rapidly absorbed from the gastrointestinal tract following the administration of 36ClO2 orally, and the half-life for the elimination of 36Cl from the rat was 44 hr, corresponding to a rate constant of 0.016/hr. After 72 hr, radioactivity was highest in plasma, followed by kidney, lung, and stomach. 36Cl in plasma reached a peak at 2 hr and 1 hr after oral administration of 36ClO2- and 36ClO3-, respectively. 36Cl excretion was greatest 24 hr after the administration of 36ClO3-, but in the case of 36ClO2-, the excretion probably represented saturation of the biotransformation and excretion pathway. A low activity in packed cells compared to plasma was detected in chlorate ingestion, rather than an even distribution in chlorite treatment. Chloroform determinations in rat blood after one year indicated that chloroform was significantly higher than control in the 100 and 1000 mg/l. ClO2 groups. However, no significant values were observed in the 1 or 10 mg/l. ClO2 and ClO2 metabolites groups. ClO2 and its metabolites are eliminated from the body more rapidly than chlorine, and they do not appear to increase trihalomethane concentrations at low dosages.
An epidemiologic study of the benefits and risks of running
Authors: J. P. Koplan
Journal: JAMA (1982)

To better estimate rates of certain benefits and risks of recreational running, we sent questionnaires to 1,250 randomly selected male and 1,250 female registrants for a 10-km road race. The response rate was 55% for men and 58% for women. Telephone interviews of a randomly selected group of nonrespondents indicated that the only significant differences between respondents and nonrespondents were that (1) respondents were older than nonrespondents, (2) more male nonrespondents had stopped running during the year after the race, and (3) more male nonrespondents had been hit by thrown objects. One year after the race; 89% of male and 79% of female respondents were still running regularly. Eighty-one percent of men and 75% of women who smoked cigarettes when they began running had stopped smoking after beginning recreational running. Giving up smoking was significantly more common for current runners than for "retired" runners. Weight loss was commonly associated with runningand was greater in those persons who were overweight when they began running. More than a third of respondents had a musculoskeletal injury attributed to running in the year after the race and about one seventh of all respondents sought medical consultation for their injury. The risk of injury increased with increasing weekly mileage. This study uses epidemiologic methods to quantify some of the benefits and risks of running. (<i>JAMA</i>1982;248:3118-3121)
PBB Fed to Adult Female Chickens: Its Effect on Egg Production, Reproduction, Viability of Offspring, and Residues in Tissues and Eggs
Authors: D. Polin, R. K. Ringer
Journal: Environmental Health Perspectives (1978)

Two experiments with light breed laying chickens fed polybrominated biphenyl (PBB) as FireMaster FF-1. The first involved feeding PBB at dietary levels of 0.2, 1, 5, 25, 125, 625, and 3125 ppm, the second involved levels of 30, 45, 60, 90, and 120. Each group had 24 hens, and each experiment had a control group of 24 hens. PBB diets were fed for 5 weeks. Feed intake, production, reproduction, tissue residues and viability of offspring were monitored during that time and a subsequent 8 weeks. Production, hatchability, and viability of offspring were significantly affected by feeding PBB at 45 ppm. Marked inanition occurred at levels of 625 and 3125 ppm, and there was some loss of feed intake at 125 ppm. There was a return to normal production and hatchability in 3 to 4 weeks after PBB withdrawal of diets with levels of 125 ppm or less. Dose--response lines are presented for PBB in muscle, liver, kidney, adipose tissue, and eggs. Withdrawal curves for PBB from these tissues are also given.
Exploring the preferences for place of death among cancer patients and their caregivers.
Authors: Marieberta Vidal, Alfredo Rodriguez Nunez, David Hui, Julio Allo, Janet L. Williams, Minjeong Park, Susan Frisbee-Hume, Eduardo Bruera
Journal: Journal of Clinical Oncology (2015)

9528 Background: Understanding the preferred place of death (POD) for advanced cancer patients (pts) is very important when they are approaching the end of life. Meeting the preferences of pts is considered an important palliative care outcome. Prior studies reported that more than 80% of pts with terminally ill cancer prefer to die at home. In many countries such as the UK, Italy, Greece, South Korea and Japan home deaths have been falling, but in some states of the US/Canada there are indications of a reversal of trends. Dying at home may be more difficult for pts in severe physical and psychosocial distress, or when there are social or financial difficulties. The purpose of this study was to determine POD preference among palliative care pts in the outpatient center (OC) and the palliative care unit (PCU). Methods: A sectional anonymous questionnaire was administered to advanced cancer pts and caregivers (PCU pts and OC) between August 2012 and September 2014. PCU pts responded when there was no delirium and the primary caregiver responded when the patient (pt) was unable to respond. In the case of outpatients dyads were assessed. Results: Overall 141/216 (65%) preferred home death. PCU pts preferred home death less than outpatients (table). Patient and caregiver agreement regarding preferred place of death was 86% (p < 0.001). The preferred POD was the same as one month ago for 82/99 (83%) PCU pts and 111/116 (96%) outpatients, with significant difference between groups (p = 0.002). Conclusions: Although home is the preferred POD in advanced cancer pts, a substantial minority prefer hospital death or no difference. Pts admitted to the PCU have a higher preference for hospital death in comparison to the outpatients, likely reflecting more severe distress because they already tried home care. Pts and caregivers agree on POD in most cases. Personalized assessment of POD preference for both patient and caregiver is needed. Preferred POD per location. Preference Total PCU* OC** P N=216 % N=100 % N= 116 % Home 141 65 58 58 83 72 0.02775 Hospital 40 19 27 27 13 11 No preference 28 13 12 12 16 14 Others 7 3 3 3 4 3 P-values are from Fisher’s exact test comparing preference of POD between PCU & OC; *PCU = Pts (N = 24) Caregivers (N = 76); ** OC = Patients (N = 116)
Editorial: Medical education, medical care, and our public
Authors: R. L. Evans
Journal: JAMA (1973)

Frustration and disquiet with medical care is a major symptom of our present culture. The American public has been deluged with information on what fine, crisis-oriented emergency care and the episode-oriented specialty care modern medicine can provide. The public has also experienced the near impossibility of obtaining a third, and less complex care—continuing, comprehensive personal care. At times, some people we serve have not been able to gain access to any nonemergent care, yet provision of all three types of care is clearly within our abilities. Difficulty in provision of such care at acceptable cost has produced three major pressures on our system of health care and medical education. These are (1) pressure for major change in the method, control and amount of third-party payments for health care, coupled with mounting ire toward supporting biomedical research and medical education from the patient care dollar; (2) increasing public and political resistance
In this series we ask one or more experts to review a case. They are provided with information on which further management is to be based, and asked to explain their rationale, using the available evidence in the literature. What was actually done in the case is then revealed. An Elderly Man with a Postcholecystectomy Bile Leak
Authors: None BaillieGUESTDISCUSSANT:T.Ponchon
Journal: Endoscopy (2000)

I am pleased to introduce Dr Thierry Ponchon, MD, from Lyon, France, as our guest discussant. Dr Ponchon is a world expert in hepatobiliary and pancreatic disorders.
At last! A medical treatment for skin aging
Authors: B. A. Gilchrest
Journal: JAMA (1988)

"We sell hope...." Only a few years ago, this much-quoted self-assessment of the cosmetic industry accurately portrayed the state of the art. No sophisticated consumer, let alone scientist, had any expectation that the unattractive concomitants of skin aging could truly be reversed. The article by Weiss and coworkers<sup>1</sup>in this issue ofThe Journalsuggests that a new age has dawned. See also p 527. The University of Michigan investigators<sup>1</sup>present strong evidence that the wrinkling, sallowness, roughness, and mottled pigmentation characteristic of habitually exposed skin of middle-aged and elderly Caucasions can be improved within a four-month period by daily topical application of all<i>trans</i>-retinoic acid (tretinoin). In each subject, the left forearm and face were randomly treated with either 0.1% tretinoin cream or vehicle cream control, while the right forearm received the other preparation. Comparisons with the pretreatment state were made on five occasions during the