tbuckley/cabot-search huggingface dataset:
Personal View
Authors: B. Bury
Journal: BMJ (1990)

<h3>Abstract</h3> Cryo-electron tomography is an emerging technique to study the cellular architecture and the structure of proteins at high resolution <i>in situ</i>. Most biological specimens are too thick to be directly investigated and are therefore thinned by milling with a focused ion beam under cryogenic conditions. This procedure is prone to frost and amorphous ice depositions which makes it a tedious process, leading to suboptimal results especially when larger batches are milled. Here, we present new hardware that overcomes the current limitations. We developed a new glove box and a high vacuum cryo transfer system and installed a stage heater, a cryo-shield and a cryo-shutter in the FIB milling microscope. This tremendously reduces the ice depositions during transfer and milling, and simplifies the handling of the sample. In addition, we tested a new software application that automates the key milling steps. Together, these improvements allow for high-quality, high-throughput cryo-FIB milling.
Conjugation of a self-antigen to papillomavirus-like particles allows for efficient induction of protective autoantibodies
Authors: Bryce Chackerian, Douglas R. Lowy, John T. Schiller
Journal: Journal of Clinical Investigation (2001)

High avidity and long-lasting autoantibodies to a self-polypeptide (TNF-α) were generated after parenteral vaccination of mice with low doses of virus-like particle–based (VLP-based) vaccines that were constructed by linking mouse TNF-α peptides to the surface of papillomavirus VLPs. High-titer autoantibodies were induced with or without coadministration of potent conventional adjuvants, but were enhanced by coadministration of CFA. Compared with immunization with the fusion protein alone, attachment to VLPs increased autoantibody titers 1,000-fold. A comparison of Ab responses against the self (TNF-α) and foreign components of the fusion protein showed that VLP conjugation abrogated the ability of the humoral immune system to distinguish between self and foreign. Similar levels of IgM were detected to self and foreign epitopes regardless of the assembly state of the antigen, suggesting that conjugation of self-peptides to VLPs promotes survival or expansion of mature autoreactive B cells. In a mouse model, vaccination with conjugated particles inhibited development of type II collagen-induced arthritis. Together, these results suggest a potentially flexible method to efficiently generate autoantibodies against specific self-proteins that mediate arthritis and other diseases.
Abstract 1429: The Proline-Rich Tyrosine Kinase PYK2 Regulates NO Production by eNOS under Physiologic and Pathophysiologic Conditions.
Authors: Annemarieke E Loot, Beate Fisslthaler, Rudi Busse, Ingrid Fleming
Journal: Circulation (2007)

In endothelial cells, the proline-rich tyrosine kinase (PYK2) is activated by fluid shear stress and phosphorylates the endothelial NO synthase (eNOS) on tyrosine residue 657. We have previously shown that eNOS phosphorylation on Tyr657 inhibits NO production in vitro and therefore addressed the consequences of PYK2 activation in a more physiological situation i.e., in perfused isolated carotid arteries as well as in response to other stimuli. Pressurised perfused murine carotid arteries subjected to step-wise increases in luminal flow responded with a graded endothelium-dependent vasodilatation that was partially sensitive to NOS inhibition. Carotid arteries in which the endothelium was infected with an adenovirus expressing a dominant-negative PYK2 mutant displayed normal flow-dependent vasodilatation (maximal response: R max 49 ± 6 μm) that was sensitive to the NOS inhibitor L-NAME (R max 28 ± 2 μm). However, flow-induced vasodilatation was markedly reduced in arteries overexpressing wild-type PYK2 and was insensitive to L-NAME (R max 27 ± 4 μm and 28 ± 5 μm, respectively) indicating loss of the NO-dependent component of vasodilatation. Given that angiotensin II is known to stimulate PYK2 phosphorylation and activation in smooth muscle cells we assessed the effects of angiotensin II on endothelial PYK2 activation and NO-dependent vasodilatation. We observed a time-dependent phosphorylation of PYK2 immuno-precipitated from porcine aortic endothelial cells stimulated with angiotensin II for 30 min up to 24 hrs. This correlated with an angiotensin II-induced decrease in NO-dependent vasodilatation. These data indicate that under physiological conditions, the tyrosine phosphorylation of eNOS by PYK2 in response to fluid shear stress attenuates the activity of the enzyme and limits NO-mediated flow-dependent vasodilatation. This PYK2-dependent inhibition of NO production may at least partially limit the detrimental consequences of maintained high NO output e.g. the generation of peroxynitrite. Conversely, under pathophysiological conditions in which angiotensin II levels are elevated, the increased activity of PYK2 can contribute to endothelial dysfunction by reducing eNOS activity.
Author Missing From Author List
Journal: JAMA (2016)

US Preventive Services Task Force; Kirsten Bibbins-Domingo, PhD, MD, MAS; David C. Grossman, MD, MPH; Susan J. Curry, PhD; Karina W. Davidson, PhD, MASc; John W. Epling Jr, MD, MSEd; Francisco A. R. García, MD, MPH; Matthew W. Gillman, MD, SM; Alex R. Kemper, MD, MPH, MS; Alex H. Krist, MD, MPH; Ann E. Kurth, PhD, RN, MSN, MPH; C. Seth Landefeld, MD; Michael LeFevre, MD, MSPH; Carol M. Mangione, MD, MSPH; Douglas K. Owens, MD, MS; William R. Phillips, MD, MPH; Maureen G. Phipps, MD, MPH; Michael P. Pignone, MD, MPH; Albert L. Siu, MD, MPH
STRIAE IN NORMAL MEN
Journal: JAMA (1966)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
New ideas in asthma and allergy research: creating a multidisciplinary graduate school
Authors: B. Bjorksten
Journal: Journal of Clinical Investigation (2003)

The spring term of 2001 saw the start of a new, unique graduate research training program at the Centre for Allergy Research at the Karolinska Institutet in Stockholm, Sweden. The program was created to bridge the gaps between basic, clinical, social, and behavioral sciences and to establish a global approach to the study of asthma and allergy. A reflection, two years on, discusses the strategies that are key to this model’s success and the challenges in introducing a multidisciplinary research program.
AMA's VISION
Journal: JAMA (1997)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Narrowed TCR repertoire and viral escape as a consequence of heterologous immunity
Authors: M. Cornberg, A. T. Chen, L. A. Wilkinson, M. A. Brehm, S.-K. Kim, C. Calcagno, D. Ghersi, R. Puzone, F. Celada, R. M. Welsh, L. K. Selin
Journal: Journal of Clinical Investigation (2007)

During the preparation of the manuscript, an error was introduced into the series of oligonucleotide primer sequences on page 1455.The sequence for the Vβ18 primer was inadvertently given for the constant region of the TCR β-chain (Cβ) as well.The correct Cβ sequence is 5'-GGCTCAAACAAGGAGACCTTGGGTGG-3'.
British Association for the Study of the Liver
Authors: G Dusheiko, H Weiss, D Brown, S-W Chan, P-L Yap, S Sherlock, N Mcintyre, P Simmonds, S Rassam, J Wilber, N Thompson, A Ridley- Dash, M Urdea, P Neuwald
Journal: Gut (1993)

It has become important to clarify whether genotypes of hepatitis C virus correlate with patho- genicity, infectivity, response to antiviral treat- ment, and geographic clustering.We have analysed the pattern of nucleotide sequence variability in the 5 prime non-coding region (5'NCR) by restricting enzymes to analyse the distribution of HCV genotypes in 80 patients with chronic hepatitis C. Genotypes were correlated with demographical, clinical, and histological features.Biopsies were coded as (1) mild or moderate chronic hepatitis without cirrhosis or (2) severe chronic hepatitis, cirrho- sis or hepatocellular carcinoma.There were 55 men and 25 women, mean age 47-5 years.Thirty nine patients were infected with type 1, 10 with type 2, eight with type 3, a further 21 were infected with a new distinct HCV type provisionally termed type 4, and two were infected with an as yet uncharacterised type (U).There was a significant association between geographical origin and genotype.Types 1, 2 or 3 were found in patients from the United Kingdom, southern Europe, Asia, Africa, and South America.Seventeen of 21 type 4 genotype isolates, however, were from Middle Eastern patients compared with 2/39 type 1 isolates (p<'005, Odds ratio 15-7).Conversely, 18/20 (90%) Middle Eastern patients had type 4 HCV v 2/20 with type 1 (p< 005, Odds ratio 9).There was no significant difference between the mean ages or mean serum aminotransferase concentrations between the various types.Types 1, 2, 3, and U were found in patients with both (1) mild to moderate disease, or (2) severe disease.Twenty two of 31 patients with type 1 who had liver biopsies had severe chronic hepatitis, cirrhosis or hepatocellular carcinoma histologically, compared with 9/31 who had mild or moderate chronic hepatitis without cirrhosis (p=004, Odds ratio 2-5).There was little definite relation between viral genotypes and response to alpha interferon treatment.Our data suggest that different HCV genotypes can be encountered in both mild and severe disease.There is a significant geographical clustering of type 4 in the Middle Eastern area.The clinical significance and prognostic importance of these genotypes may require prolonged follow up studies.
Help Your Doctor to Help You When You Have Food Allergy
Journal: JAMA (1942)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
PROCEEDINGS OF THE SEVENTEENTH ANNUAL MEETING OF THE AMERICAN SOCIETY FOR CLINICAL INVESTIGATION HELD IN WASHINGTON, D. C., MAY 4, 1925
Journal: Journal of Clinical Investigation (1925)

Continuing the work of Parker it has been possible to cultivate vaccine virus in artificial media at 37.5°C.for 130 days.The medium is essentially a living tissue culture, consisting of a small bit of normal rabbit testis to which is added infected testis (or plasma), the whole being held in place by one or two drops of diluted normal rabbit plasma.The cultures are made in small Petri dishes (5 cm.diameter) and after coagulation of the plasma the dishes are inverted and sealed with sterile vaseline.Transplants are made every five or six days.Analy- sis of the air in the Petri dishes after five to six days incubation shows an oxygen content comparable to that of normal air.Intradermal titrations in rabbits show a marked increase in the actual amount of virus from generation to generation, thus eliminating the possibility of mere survival.
Dermabrasion
Authors: E. H. DeKleine
Journal: JAMA (1969)

<h3>To the Editor:—</h3> Dr. Ervin Epstein's "Present Status of Dermabrasion" (<b>206</b>:607, 1968) states that dermabrasion can no longer be considered a useful means for the treatment of acne scars. Most of the complications that Dr. Epstein cites: bleeding, pain, erythema, infection, and milia are infrequent or transient. The problem of scarring is rare and only results if improper technique is used. The major complications are pigmentary disturbances, which are fortunately uncommon, and the result of over-exposure to sunlight, especially when the exposure occurs shortly after the dermabrasion. No one will deny that acne scars can be disfiguring and can cause severe psychological consequences. Even the most enthusiastic supporters of dermabrasion will admit that perfect results cannot be attained, but this is no reason to discard a procedure which can achieve a marked cosmetic improvement. Because of the less than perfect results from dermabrasion, some of the initial enthusiasm for
Idiopathic Hypoventilation
Authors: Joseph L. Grant
Journal: JAMA (1965)

Thin as well as fat people may chronically underbreathe and, though their lungs are normal, the resulting hypoxia may lead to pulmonary hypertension and even to cor pulmonale. Cardiac therapy may then allow a level of breathing which partly corrects hypoxia and hypercapnia, thus breaking the vicious cycle which contributed to the heart failure initially. One cannot treat the lesion in the medullary respiratory center, but treatment of heart failure, artificial ventilation, and oxygen may, as in the present case, permit the patient to return to work. Prevention of heart failure then allows a level of breathing which partly corrects anoxia and hypercapnia and, in turn, prevents recurrent episodes of heart failure.
Induced termination of pregnancy before and after Roe v Wade. Trends in the mortality and morbidity of women. Council on Scientific Affairs, American Medical Association
Authors: Yank D. Coble, E. Harvey Estes, C. Alvin Head, Mitchell S. Karlan, William R. Kennedy, Patricia Joy Numann, William C. Scott, W. Douglas Skelton, Richard M. Steinhilber, Jack P. Strong, Christine C. Toevs, Henry N. Wagner, Jerod M. Loeb, Robert C. Rinaldi, Janet E. Gans
Journal: JAMA (1992)

The mortality and morbidity of women who terminated their pregnancy before the 1973 Supreme Court decision in Roe v Wade are compared with post-Roe v Wade mortality and morbidity. Mortality data before 1973 are from the National Center for Health Statistics; data from 1973 through 1985 are from the Centers for Disease Control and The Alan Guttmacher Institute. Trends in serious abortion-related complications between 1970 and 1990 are based on data from the Joint Program for the Study of Abortion and from the National Abortion Federation. Deaths from illegally induced abortion declined between 1940 and 1972 in part because of the introduction of antibiotics to manage sepsis and the widespread use of effective contraceptives. Deaths from legal abortion declined fivefold between 1973 and 1985 (from 3.3 deaths to 0.4 death per 100,000 procedures), reflecting increased physician education and skills, improvements in medical technology, and, notably, the earlier termination of pregnancy. The risk of death from legal abortion is higher among minority women and women over the age of 35 years, and increases with gestational age. Legal-abortion mortality between 1979 and 1985 was 0.6 death per 100,000 procedures, more than 10 times lower than the 9.1 maternal deaths per 100,000 live births between 1979 and 1986. Serious complications from legal abortion are rare. Most women who have a single abortion with vacuum aspiration experience few if any subsequent problems getting pregnant or having healthy children. Less is known about the effects of multiple abortions on future fecundity. Adverse emotional reactions to abortion are rare; most women experience relief and reduced depression and distress.
Erratum: “Low-Level Environmental Lead Exposure and Children’s Intellectual Function: An International Pooled Analysis”
Authors: Bruce P. Lanphear, Richard Hornung, Jane Khoury, Kimberly Yolton, Peter Baghurst, David C. Bellinger, Richard L. Canfield, Kim N. Dietrich, Robert Bornschein, Tom Greene, Stephen J. Rothenberg, Herbert L. Needleman, Lourdes Schnaas, Gail Wasserman, Joseph Graziano, Russell Roberts
Journal: Environmental Health Perspectives (2019)
Implication of Oxygen-Regulated Protein 150 (ORP150) in Apoptosis Induced by Proteasome Inhibitors in Human Thyroid Cancer Cells
Authors: Yan-Yan Gao, Bao-Qin Liu, Zhen-Xian Du, Hai-Yan Zhang, Xiao-Fang Niu, Hua-Qin Wang
Journal: Endocrine Reviews (2010)

ABSTRACT Context The inhibition of the 26S proteasome may lead to endoplasmic reticulum stress, which has been shown to be implicated in the antitumoral effects of proteasome inhibitors. Oxygen-regulated protein 150 (ORP150) is an inducible endoplasmic reticulum chaperone that is up-regulated after numerous cellular insults and has a cytoprotective role for the maintenance of cellular viability. Objective The purpose of this study was to determine the involvement of ORP150 in cytotoxicity of thyroid cancer cells mediated by proteasome inhibition. Design The effects of proteasome inhibition on the expression of ORP150 were analyzed using real-time RT-PCR and Western blot. To ascertain the effect of ORP150, cells were transfected with ORP150 plasmid or small interfering RNA (siRNA) against ORP150, apoptotic cells, and induction of CCAAT/enhancer-binding protein homologous transcription factor (CHOP) mediated by proteasome inhibition were investigated. Results ORP150 was induced in thyroid cancer cells after proteasome inhibition. Suppression of activating transcription factor 4 expression by siRNA inhibited the up-regulation of ORP150 mediated by proteasome inhibitors. siRNA for ORP150 stimulated MG132-mediated apoptosis and induction of CHOP, a transcription factor with apoptosis-inducing activity. In contrast, ORP150-overexpressing cells demonstrated less susceptibility to MG132-induced apoptosis and displayed less up-regulation of CHOP. In addition, the sensitizing effect of small interfering ORP150 on apoptosis was suppressed by siRNA for CHOP. Conclusions These results suggest that up-regulation of ORP150 in thyroid cancer cells inhibits MG132-induced apoptosis via suppression of CHOP induction, thereby decreasing the potential antitumor activity of MG132.
05.04 Functional polymorphisms of the abcg2 gene in primary gout
Authors: Blanka Stiburkova, Katerina Pavelcova, Lenka Petru, Pavel Cepek, Jakub Zavada, Karel Pavelka
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> The urate transporters are one of the genetic determinants of serum uric acid concentrations. Common dysfunctional variants of <i>ABCG2</i> are revealed to be a major cause of gout and hyperuricemia by decreasing urate excretion. In the present study we describe the analysis of allelic variants in the <i>ABCG2</i> gene in a cohort with primary gout. <h3>Methods</h3> The cohort consisted of 143 individuals (129 men, 14 women). Gouty arthritis was diagnosed according to the 1977 preliminary criteria of the American College of Rheumatology. Patients suffering from secondary gout were excluded. All 16 exons of <i>ABCG2</i> were amplified using PCR and sequenced directly. <h3>Results</h3> In the <i>ABCG2</i> gene, 16 intronic variants were detected. In the case of c.689+1G&gt;A, related to an individual with severe gouty phenotype, two abnormal splicing variants were identified lead to frameshift and premature stop codon introduction.<sup>1</sup> From the ten exon variants detected, there were eight non-synonymous: p.V12M, p.Q141K, p.R147W, p.T153M, p.F373C, p.T434M, p.S476P and p.D620N. Heterozygous p.V12M was detected in seven individuals. Heterozygous variants p.R147W, p.T153M, p.F373C, p.T434M and p.S476P were detected once, variant p.D620N twice. All these allelic variants were <i>in silico</i> predicted as a probably damaging and were not detected in control cohort of 150 normouricemia subjects. The p.Q141K, previously functionally characterised allelic variant with a strong effect on uric acid secretion impairment, was in cohort of gout patients presented with significantly higher minor allele frequency (MAF=0.19, 43 heterozygotes/5 homozygotes), than in population of European origin (MAF=0.09). In our cohort, the age of onset of gout had a normal distribution. Remarkably, in 75% of all individuals with early onset of gout between age 10–20 years, we detected p.Q141K (in 6 out of 8 patients). In the age group 21–30 years, p.Q141K was detected in 42% of patients (8/19), and in the age group 61–70 years, just in 29% (13/29). <h3>Conclusions</h3> Our results show that genetic factor <i>ABCG2</i> should be considered as one of the strong common risks for gout. In summary, we revealed that allelic variants of <i>ABCG2</i>, especially dysfunctional variant p.Q141K, have a significant effect on earlier onset of gout. <h3>Reference</h3> 1. Stiburkova B, Miyata H, Závada J, <i>et al</i>. Novel dysfunctional variant in <i>ABCG2</i> as a cause of severe tophaceous gout: biochemical, molecular genetics and functional analysis. Rheumatology (Oxford). 2016Jan;55(1):191-4. This study was supported by the grants from the Czech Republic Ministry of Health AZV 15–26693A.
Clinical haematology.
Journal: BMJ (1969)

MBITLSOUNL 795 vaccination in Britain.Finally, the incident is a further reminder of the necessity of adequate surveillance of all vaccination procedures, including that against measles.As was previously said in these columns2 before the measles vaccination campaign was launched in Great Britain, it is essential that a close watch should be kept on the effects of vaccination.Only in this way can a true assessment of the vaccine in general use be obtained, and it is important that the Departments of Health should ensure that a well-planned and long-term surveillance programme is carried out.