tbuckley/cabot-search huggingface dataset:
Abstract 152: SOX2-mediated 5hmC dysregulation in GBM stem cells
Authors: Hernando M. Lopez-Bertoni
Journal: Cancer Research (2020)

Abstract Primary brain tumors are among the most devastating forms of cancer and glioblastoma (GBM) represents the most aggressive and lethal form of the disease. We now know that GBM contain small subsets of cells that display tumor-propagating stem-like phenotypes (i.e. glioma stem cells or GSCs) that act as critical determinants of GBM resistance to current treatments and tumor recurrence for which there is no proven therapy. Altered patterns of DNA methylation are widely reported in human GBM. However, substantial knowledge gaps remain in our understanding of the molecular mechanisms responsible for this epigenetic dysregulation, its downstream consequences and role in the GBM tumor propagating phenotype. Understanding and ultimately targeting the epigenetic mechanisms that induce and maintain these tumor-propagating cell subsets is critical to improving GBM therapy and patient outcomes. DNA methylation generally occurs in cytosine-guanine (CpG) sequences and is established by DNMTs, which catalyze the conversion of cytosine to 5-methylcytosine (5mC). DNA methylation is a reversible process and is partially mediated by the ten-eleven translocation (TET) family of enzymes which function as deoxygenases to catalyze the conversion of 5mC to 5-hydroxymethylcytosine (5hmC). Multiple studies found negative correlations between 5hmC levels and glioma grade and loss of 5hmC correlates with poor prognosis of GBM patients. TET enzymes and regulation of 5mC and 5hmC have the capacity to function on a global scale to regulate tumor suppressing mechanisms. We have found that SOX2 represses the TET2 demethylase and decreases 5hmC, the enzymatic product catalyzed by TET proteins, in GSCs. TET2 repression using 2 independent shRNA hairpins efficiently decreases 5hmc levels and significantly enhances self-renewal capacity and tumor growth capacity of low-passage GSC isolates. We also show that SOX2 induces expression of miR-10b-5p, a known onco-miR predicted to target TET2, and inhibiting this miRNA rescued the reduction in 5hmC expression observed in GSCs, thus identifying miR-10b-5p as a critical mediator of SOX2-induced onco-methylation, GSC induction and glioma malignancy. Our results identify a novel targetable pathway by which SOX2 regulates the epigenetic landscape of GBM-propagating cells. This novel mechanism combined with our recent reports that SOX2 regulates DNMTs and the HMGA1 chromatin remodeling protein identified a multi-dimensional epigenetic mechanism by which oncogenic signals control GBM cell stemness and tumor propagation. Citation Format: Hernando M. Lopez-Bertoni. SOX2-mediated 5hmC dysregulation in GBM stem cells [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 152.
Risky Business
Journal: JAMA (1994)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Cerebral phosphorus-31 magnetic resonance spectroscopy in patients with chronic hepatic encephalopathy
Authors: S Taylor-Robinson
Journal: Hepatology (1994)

Cerebral phosphorus-31 magnetic resonance spectroscopy was undertaken in 33 patients with biopsyproven cirrhosis: 6 had no evidence of neuropsychiatric impairment on standard clinical, psychometric and electrophysiological testing; 8 had evidence of subclinical hepatic encephalopathy; and 19 were classified as having overt hepatic encephalopathy. The reference population comprised 15 healthy volunteers. Unlocalized spectra were acquired from the entire head with a 45-degree pulse angle and repetition times of 1 and 5 sec. Spectra localized to the basal ganglia were acquired with a 45-degree pulse angle and a repetition time of 1 sec. Peak area ratios of phosphomonoesters, inorganic phosphate, phosphodiesters and phosphocreatine relative to β-ATP were measured in the spectra acquired. We noted no consistent change in the ratios of inorganic phosphate to ATP and phosphocreatine to ATP. Mean values of the ratios of phosphomonoesters to ATP and phosphodiesters to ATP were significantly lower in the total patient population than in the reference population, and they correlated with the patients' neuropsychiatric status. Thus we found no significant reductions in the mean ratios of phosphomonoesters to ATP and phosphodiesters to ATP in patients who were neuropsychiatrically unimpaired, but significant reductions were observed in the mean ratios of phosphomonoesters to ATP and phosphodiesters to ATP in patients with both subclinical and overt hepatic encephalopathy. The most marked reductions in these metabolite ratios were observed in patients with overt encephalopathy. (Hepatology 1994;20:1173–1178).
The Heart and the Aorta. Studies in Clinical Radiology.
Journal: JAMA (1921)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Evidence that cholesterol antibodies exist in human gallbladder bile and may be promoters of nucleation , Washington University School of Medicine, St Louis, MO
Journal: Hepatology (1995)

In earlier work we showed that cholesterol monohydrate crystallization from model and native biles can involve filamentous cholesterol crystals, and other metastable intermediates which are covered by a layer of phosphatidylcholine (lecithin) molecules [Konikoff et al., J. Clin. Invest. 90 (1992) 1155]. The aim of the present study was to isolate the initial filamentous cholesterol crystals by density gradient centrifugation and microfiltration and to sequentially monitor their transformations into equilibrium plates within the mother liquor composed of a dilute (1.2 g/dl) bile salt-rich model bile (cholesterol/egg yolk lecithin/sodium taurocholate, 1.7/0.8/97.5 mol%). When assayed by dual radiolabeling at 37°C, total precipitated cholesterol in bile increased from zero at 2–4 h of incubation to 43% at 24 h, reaching a stable value by 48 h when 36% of total cholesterol had crystallized. Isopycnic sucrose density gradient centrifugation at 20°C separated early filamentous crystals from plate-like crystals and revealed densities compatible with anhydrous cholesterol (1.029 g/ml) and cholesterol monohydrate (1.048 g/ml), respectively. Rapid (1 h) density gradient centrifugation carried-out in time-lapse sequence disclosed that cholesterol crystallization involved initially low-density (1.01-1.03 g/ml) filamentous crystals, which reached a maximal concentration at 24 h and disappeared gradually by 156 h of incubation. Concomitantly, the concentrations of high-density (1.04-1.06 g/ml) plate-like cholesterol crystals increased reciprocally throughout the crystallization process suggesting a precursor-product relationship. Rates of crystal filament formation and transitions to thermodynamically stable plates accelerated curvilinearly with increases in temperature from 4 to 60°C, but the crystallization process per se remained unchanged. We conclude that metastable intermediate crystals during cholesterol precipitation from bile may involve either low-density anhydrous crystals or a new cholesterol polymorph that transforms slowly at physiologic temperature (37°C) into classic plate-like cholesterol monohydrate crystals. Clearly, cholesterol crystallization in model bile is more complex and heterogenous than hitherto believed and monitoring metastable intermediate forms, habits and possibly polymorphs should provide a better framework for studying the physical chemistry of nucleation and crystal growth in native bile as well as promoters and inhibitors of these processes.
Cholera, Its Increase in Paris.
Journal: JAMA (1884)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Correction
Journal: BMJ (1947)

MEDICAL JOURNAL take 1,500 million litres of the average spa water to reach a dangerous dose.Ointments containing radium salts may be dangerous, but do not now seem to be used.Emanotherapy is not included in the general regimen of treatment of rheumatic subjects in this country; spa treatment is used for the discipline incurred and for its value as a remedial agent in muscular re-education.Fitch, writing on mineral waters of the U.S.A., states that sciatica is curable by radio-active treatment, if it is continued long enough, by baths and springs.Such a state- ment, reviewed in the light of modern pathology, leads to increased confidence in time, but not in radio-activity, as a cure, and such criticism is applicable to many aspects of this controversial subject.
Book Listings
Journal: Annals of Internal Medicine (2001)

Book Listings3 April 2001Book ListingsSearch for more papers by this authorhttps://doi.org/10.7326/0003-4819-134-7-200104030-00006 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail AngiologyVein Diagnosis and Treatment: A Comprehensive ApproachRA Weiss, C Feied, and MA Weiss, eds. 304 pages. New York: McGraw-Hill; 2001. $149.00. ISBN 0070692017. Order phone 800-262-4729.CardiologyAcute Coronary Syndromes2nd edition. EJ Topol, ed. 862 pages. New York: Marcel Dekker; 2001. $100.00. ISBN 0824704169. Order phone 800-228-1160.Angiotensin I Receptor AntagonistsM Epstein and HR Brunner, eds. 384 pages. Philadelphia: Hanley & Belfus; 2001. ISBN 1560534532. Order phone 800-962-1892.Antioxidants and Cardiovascular DiseaseJ Tardif and MG Bourassa, eds. 258 pages. Boston: Kluwer; 2000. $95.00. ISBN 079236564X. Order phone 781-871-1660.Blood Pressure Monitoring in Cardiovascular Medicine and TherapeuticsWB White, ed. 308 ... PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 3 April 2001Volume 134, Issue 7Page: B1KeywordsPrevention, policy, and public health ePublished: 3 April 2001 Issue Published: 3 April 2001 Copyright & PermissionsCopyright © 2001 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Thalassemia genes.
Authors: P Wasi
Journal: BMJ (1970)

atrium.A convenient external reference point for this in a supine patient is the mid axillary point.This level was presumably used by A. E. Baue, whose review we quoted.Some prefer to use the sternal angle as a reference level, in which case the recorded pressures will, of course, be lower.Since the usefulness of C.V.P. measurements is not in their absolute values but in changes of C.V.P. that take place it is of little importance which reference point is chosen, provided the same point is used consistently.-ED., B.
Demyelinating Diseases
Authors: George A. Schumacher
Journal: New England Journal of Medicine (1960)

Pathological Investigations Contributing to Classification and PathogenesisContributions of Descriptive Pathology Vascular factors. For many years the existence of primary vascular lesions in multiple sclerosis has been debated. A large body of literature on this subject up to 1950 will not be summarized here. A study of the topographic distribution of plaques in the spinal cord in multiple sclerosis91 showed a vascular pattern with the distribution of plaques related to the spinal veins and their drainage territories. A peculiar predilection for certain patterns of localization of spinal plaques was described. In a detailed study of the pathology of multiple . . .
Abstract 486: MicroRNA-30 family members inhibit breast cancer invasion, osteomimicry, and bone destruction by directly targeting multiple bone metastasis-associated genes
Authors: Martine Croset, Francesco Pantano, Casina Kan, Edith Bonnelye, Francoise Descotes, Catherine Alix-Panabières, Charles Lecellier, Richard Bachelier, Nathalie Allioli, Saw See Hong, Kai Bartkowiak, Klaus Pantel, Philippe Clézardin
Journal: Cancer Research (2018)

Abstract MicroRNAs (miRNAs), which are master regulators of gene expression, have emerged as key players in cancer metastasis. Here, we established miR-30 family members (miR-30a, miR-30b, miR-30c, miR-30d and miR-30e) as breast cancer bone metastasis suppressor genes. Low expression levels of miR-30s in primary tumors of breast cancer patients were associated with poor relapse-free survival. Additionally, miR-30s levels were significantly lower in estrogen receptor (ER)-negative/progesterone receptor (PR)-negative tumors than in their hormone-responsive counterparts. Overexpression of miR-30s in ER/PR-negative breast cancer cells resulted in the reduction of bone metastasis burden in vivo. MiR-30s overexpression also reduced tumor outgrowth, when tumor cells were implanted subcutaneously in animals. In vitro, miR-30s did not affect tumor cell proliferation, but inhibited tumor cell invasion. Furthermore, miR-30s restored bone homeostasis by reversing the in vitro effects of the tumor cell conditioned medium on stimulation of osteoclastogenesis and inhibition of osteoblastogenesis. We identified a number of genes associated with osteoclastogenesis stimulation (IL-8, IL-11), osteoblastogenesis inhibition (DKK-1), tumor cell osteomimicry (RUNX2, CDH11) and invasiveness (CTGF, ITGA5, ITGB3) that were direct and/or indirect targets for repression by miR-30s. Among these genes, integrin ITGA5 was a previously unknown miR-30 target. By silencing ITGA5 in ER-/PR-negative breast cancer cells, colonization of the bone marrow by tumor cells was drastically reduced in vivo. Overall, our data indicate that miR-30s employ multiple mechanisms to impede breast cancer bone metastasis. These findings may pave the way to a new field of therapeutic interventions in breast cancer patients with bone metastasis. Citation Format: Martine Croset, Francesco Pantano, Casina Kan, Edith Bonnelye, Francoise Descotes, Catherine Alix-Panabières, Charles Lecellier, Richard Bachelier, Nathalie Allioli, Saw See Hong, Kai Bartkowiak, Klaus Pantel, Philippe Clézardin. MicroRNA-30 family members inhibit breast cancer invasion, osteomimicry, and bone destruction by directly targeting multiple bone metastasis-associated genes [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 486.
Author Index
Authors: Ranan Rimón, Reima Kampman, Urpu Ikonen, Mauri Reunanen, Rafaella M.A. Osti, Giancarlo Trombini, Bruno Magnani, Gordon D. Jensen, Lorne M. Hartman, Bert Garssen, Harold L. Levitan, Peter E. Sifneos, Roberta J. Apfel, Ellen Bassuk, George Fishman, Andrew Gill
Journal: Psychotherapy and Psychosomatics (1980)
Supplement 386
Journal: BMJ (1911)

The authorities of this inland health resort had a stall at Birmingham chiefly devoted to the interests of what is practically a new enterprise on their part.This is the sale of Buxton water in a bottled form; the main object in view is to secure the use of the water for table purposes; so far the effort has proved even unexpectedly successful.Tile spring used is that arising at the bottom of St. Ann's well.Indications of the general advantages with which Nature has supplied Buxton were also afforded by the stall while we were informed by the official in charge that the number of different treatments of a hydropathic order available at the bathing establishment has now been brought up to over eighty.The latest additions include arrangements for " Greville " hot air and " Miramond" treatment.This spa therefore being fully up to date in all directions feels confident that it will at least hold its own despite its many rivals abroad and at home.A. WM ULFING AND COMPANY (12, Chenies Street, London, W.). Of the three exhibits of this firm the most important was Albulactin, a preparation which when first brought out a few years ago, seemed to us decidedly worth attention.Its point of interest is that it is not an addition to the multiplicity of infant foods constantly being pressed on the notice of medical men, but a preparation which in theory, at any rate, represents quite a new departure.It has been known for many years that the proteid of milk is composite, but comparatively little attention has been paid to this fact, and practically no use could be made of the knowledge very easily until a method of isolating lact- albumin, as distinct from casein, in a soluble form had been devised.This having been effected some three or four [3F6]
Abstract 3497: Low Serum Estradiol and Cardiovascular Disease Mortality in Older Men: The Rancho Bernardo Study
Authors: Gail A Laughlin, Elizabeth Barrett-Connor, Jaclyn Bergstrom
Journal: Circulation (2007)

Context: The traditional view that the male excess in cardiovascular disease (CVD) mortality is due to harmful effects of testosterone has been challenged. In older men, low, rather than high, testosterone predicts atherosclerotic disease progression, and serum estradiol, not testosterone, is prospectively associated with CVD events. Objective: To determine the association of endogenous sex hormones with cardiovascular disease mortality in older community-dwelling men. Methods: Prospective population-based study of 858 men aged 50 to 91 years (median 73) who had serum estradiol and testosterone measurements at baseline (1984 – 87) and were followed for mortality through July, 2006. Results: Median (95% interval) hormone levels were 300 (175, 505) ng/dl for testosterone and 20 (11, 32) pg/ml for estradiol. During the 22 year (average 12.6) follow-up, 559 deaths occurred; 281 (50%) were due to CVD. Age-adjusted Cox regression analyses using hormone quintiles showed testosterone was not related to CVD mortality, but suggested a threshold effect for lower levels of estradiol (P &lt; .001). The risk of CVD death was 1.77 (95% CI 1.32, 2.38) for men in the lowest estradiol quintile (&lt; 15 pg/ml) compared to those with higher levels (P &lt; .001), adjusting for age, BMI, current smoking, physical activity and alcohol intake. This association was independent of the metabolic syndrome, diabetes and prevalent cardiovascular disease. Additional adjustment for lipids, lipoproteins, blood pressure, insulin resistance, CRP, IL-6, leptin, adiponectin or androgen deficiency (total testosterone &lt; 250 ng/dl) had minimal effect on results. Exclusion of deaths that occurred during the first 2 years (to minimize the effect of occult disease) or adjustment for a combination of health status markers also had no effect. Men with low estradiol had lower BMI, smaller waist girth, and were more likely to have lost 10 pounds in the previous 10 years (all P &lt; .001); adjusting for these factors did not influence results. Conclusions: Low serum estradiol in older men is associated with increased risk of cardiovascular death, independent of age, adiposity, and lifestyle choices. This relationship does not appear to be due to pre-existing disease and is not related to androgen deficiency.
The Package Insert
Journal: JAMA (1969)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal