tbuckley/cabot-search huggingface dataset:
165. Enhanced Baculovirus-Mediated Transduction of Human Cancer Cells by Tumor- Homing Peptides
Authors: Anna R. Makela, Heli Matilainen, Daniel White, Christian Oker-Blom
Journal: Molecular Therapy (2006)

Tumor cells and tumor vasculature both offer specific molecular targets that can be utilized for site-directed delivery of therapeutic genes. In order to achieve tumor-specific gene delivery, the tropism of baculovirus can be manipulated by modification of the virus envelope using baculovirus display technology. We genetically engineered a series of recombinant display viruses by fusing various tumor-homing peptides to the transmembrane anchor of vesicular stomatitis virus G-protein to establish more efficient and selective baculovirus vectors for targeted gene delivery into human cancer cells. The viruses were further equipped with a luciferase expression cassette, allowing transduction monitoring in mammalian cells by measuring luciferase activity. The fusion proteins were successfully incorporated into budded virions and these modified viruses showed significantly improved binding to both human breast carcinoma (MDA-MB-435) and hepatocarcinoma (HepG2) cells as measured by flow cytometry. Binding of the tumor-homing peptide displaying viruses to target cells was reduced to the level of the control virus by preincubating the cells with the corresponding soluble peptides. Moreover, a maximum of 7- and 24-fold increase in transgene expression was achieved for these cell lines, respectively. The enhanced binding and transduction strongly suggest that the displayed peptides dictated this behavior. Together, these results imply that the specificity and efficiency of baculovirus-mediated gene delivery can be notably enhanced in vitro when tumor-targeting ligands are used and therefore also highlight the potential of baculovirus vectors in cancer gene therapy in vivo.
Single-dose amoxicillin therapy for urinary tract infection. Multicenter trial using antibody-coated bacteria localization technique
Authors: R. H. Rubin
Journal: JAMA (1980)

Urine specimens from 134 women with acute, uncomplicated urinary tract infection at three medical centers were examined by the antibody-coated bacteria (ACB) assay. Patients with negative assays (suggesting bladder infection alone) were randomized to receive either a single 3-g oral dose of amoxicillin trihydrate or conventional ten-day courses of sulfamethoxazole-trimethoprim or oral ampicillin sodium. Comparable results were obtained with the three regimens for ACB-negative infection: 90% eradication of the original organism with single-dose amoxicillin, 100% with sulfamethoxazole-trimethoprim, and 96% with ampicillin. The overall incidence of ACB positivity was 32.1%, ranging from 8% to 63% at the three institutions. This difference seemed to be related to the ease of access to medical care: women with easy access having low rates of ACB positivity and those with poor access having high rates. (<i>JAMA</i>244:561-564, 1980)
Epidemic Encephalitis: Etiology, Epidemiology, Treatment, Report of a Survey by the Matheson Commission.
Journal: JAMA (1930)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
For Harriet
Authors: Richard Schiffman
Journal: JAMA (2011)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The Patient
Authors: Leland S. McKittrick
Journal: New England Journal of Medicine (1957)

I FEEL very humble in having my name added to those of the illustrious men who have preceded me as Shattuck Lecturers. It was not difficult to accept this honor, but efforts to meet its challenge have not been easy.I have given careful thought to the selection of my subject. Most of my predecessors, by virtue of their noteworthy contributions, have discussed various aspects of the scientific developments in medicine. Probably because of my inability to add to those, and since I was given complete freedom of choice, I have elected to attempt to develop this lecture around those . . .
First-Line Atezolizumab Effective in Bladder Cancer
Journal: Cancer Discovery (2016)

Results from a phase II study indicate that the PD-L1 inhibitor atezolizumab, recently approved for advanced bladder cancer that's refractory to standard platinum chemotherapy, is effective as first-line therapy for this disease. Durable responses to atezolizumab were seen in nearly a quarter of the study patients, who were all ineligible to receive cisplatin.
Erratum: The endothelial protein PLVAP in lymphatics controls the entry of lymphocytes and antigens into lymph nodes
Authors: Pia Rantakari, Kaisa Auvinen, Norma Jäppinen, Maria Kapraali, Joona Valtonen, Marika Karikoski, Heidi Gerke, Imtiaz Iftakhar-E-Khuda, Johannes Keuschnigg, Eiji Umemoto, Kazuo Tohya, Masayuki Miyasaka, Kati Elima, Sirpa Jalkanen, Marko Salmi
Journal: Nature Immunology (2015)
Botulinum toxin
Authors: D G. Davies, R S Dhillon
Journal: BMJ (1989)

of live births and stillbirths large enough to produce several examples of unexplained still- births and sudden infant death syndrome and (b) routine testing of different types of haemo- globin and immunoglobulins in infants with leukaemia or severe pulmonary infections.Finally, if these tests succeeded in establishing a link between juvenile myeloid leukaemia and sudden infant death syndrome then steps could be taken to discover whether the cancer inhibitor effects of immunisations observed in the Oxford survey data'0 have counterparts in sudden infant death syndrome.This would necessitate giving immunisations to infants with abnormally high levels of fetal haemoglobin and comparing (under the usual conditions ofcontrolled clinical trials) the frequency of sudden infant death syndrome in infants with and without these boosts to their immune systems.
Fatal bronchospasm after topical lignocaine before bronchoscopy:: Author's reply
Authors: J. G Ayres
Journal: BMJ (1987)

returning to paid work was a function of prior level of mental ill health.Fourthly, other research in this unit shows that in areas of higher unemployment compared with those of lower unemployment unemployed people report feeling less stigmatised by the lack of a job, having more social contact with other unemployed people, and feeling less under pressure from other people to get a job.Differential selection of unfit people in different areas is therefore unlikely to account for our findings.
Treatment of Childhood Acute Lymphoblastic Leukemia: Results of Dana-Farber ALL Consortium Protocol 87-01
Authors: Jean Marie LeClerc, Amy L. Billett, Richard D. Gelber, Virginia Dalton, Nancy Tarbell, Jeffrey M. Lipton, Ronald Barr, Luis A. Clavell, Barbara Asselin, Craig Hurwitz, Marshall Schorin, Steven E. Lipshultz, Lieven Declerck, Lewis B. Silverman, Harvey J. Cohen, Stephen E. Sallan
Journal: Journal of Clinical Oncology (2002)

To improve efficacy and reduce toxicity of treatment for children with acute lymphoblastic leukemia.Patients from all risk groups, including infants and those with T-cell disease, were treated between 1987 and 1991. Standard-risk (SR) patients did not receive cranial irradiation, whereas high-risk (HR) and very high-risk (VHR) patients participated in a randomized comparison of 18 Gy of cranial irradiation conventionally fractionated versus two fractions per day (hyperfractionated).At a median follow-up of 9.2 years, the 9-year event-free survival (EFS +/- SE) was 75% +/- 2% for all 369 patients, 77% +/- 4% for the 142 SR patients, and 73% +/- 3% for the 227 HR/VHR patients (P =.37 comparing SR and HR/VHR). The CNS, with or without concomitant bone marrow involvement, was the first site of relapse in 19 (13%) of the 142 SR patients: 16 (20%) of 79 SR boys and three (5%) of 63 SR girls. This high incidence of relapses necessitated a recall of SR boys for additional therapy. CNS relapse occurred in only two (1%) of 227 HR and VHR patients. There were no outcome differences found among randomized treatment groups. Nine-year overall survival was 84% +/- 2% for the entire population, 93% +/- 2% for SR children, and 79% +/- 3% for HR and VHR children (P <.01 comparing SR and HR/VHR).A high overall survival outcome was obtained for SR patients despite the high risk of CNS relapse for SR boys, which was presumed to be associated with eliminating cranial radiation without intensifying systemic or intrathecal chemotherapy. For HR/VHR patients, inability to salvage after relapse (nearly all of which were in the bone marrow) remains a significant clinical problem.
Sunlamps and the risk of melanoma
Authors: F M Allwright
Journal: BMJ (1988)

and not be limited to conventions.Classical power determination does use a single alternative hypothesis, but power of the test is even greater if the true difference is larger than the posited clinically important difference.We think that Bayesian approaches offer great potential.As presently developed, however, the Bayesian method is difficult even for Bayesian statisticians and requires more research before it becomes practical.Development of Bayesian in- ference instruments that are easily employed by clinicians would greatly advance the usefulness of the Bayesian approach.
Role of microtubuli in secretion of very-low-density lipoprotein in isolated rat hepatocytes: Early effects of thiol reagents
Authors: J Nagelkerke
Journal: Hepatology (1991)

The secretion of very-low-density lipoprotein from hepatocytes proceeds through the microtubules. In this study, the role of glutathione in the maintenance of intact microtubules and the secretion of very-low-density lipoprotein has been investigated. When rat hepatocytes were incubated with reagents that deplete glutathione (e.g., diethylmaleate, α-bromoisovalerylurea or allyl alcohol) or reacted directly with protein thiols (disulfiram), the secretion of very-low-density lipoprotein by the cells was inhibited and the microtubules were severely damaged as visualized by immunofluorescence staining. Both events occurred within 30 min; long before, an effect on the energy status of the cells became evident. The observed inhibition of the secretion therefore seems due to an effect of the toxicants on the microtubules. For α-bromoisovalerylurea, diethylmaleic acid and allyl alcohol, it may be related to glutathione depletion; preincubation of the hepatocytes with N-acetyl-L-cysteine reduced the decrease of glutathione by α-bromoisovalerylurea and allyl alcohol (but not of diethylmaleic acid) and almost completely prevented the inhibition of very-low-density lipoprotein secretion and microtubule damage. Depletion of glutathione may result in modification of a small group of essential free protein thiols. Disulfiram did not deplete glutathione, and N-acetyl-L-cysteine could not prevent the effects of disulfiram on microtubules. The binding to protein thiols of radiolabeled disulfiram, which binds to microtubules in vitro, was determined. At 0.2 mmol/L disulfiram, only 3% of total cellular protein thiols were conjugated, but secretion of very-low-density lipoprotein was already inhibited by 25%, and microtubules were severely affected. We propose that modification of a small fraction of cellular protein thiols results in the loss of microtubular ultrastructure and thereby leads to inhibition of very-low-density lipoprotein secretion. (HEPATOLOGY 1991;14:1259–1268.)
Highlights
Journal: JAMA Surgery (2017)

Hospital Readmission Following Appendectomy in Children 1106Appendectomies are the most common surgical procedures for children in the United States.With increasing trends towards same-day discharge after surgery, Cairo et al evaluated wound complications and 30-day readmissions based on discharge timing using the American College of Surgeons National Surgical Quality Improvement Program pediatric database.Discharge timing was not found to be associated with readmissions or wound complications and may be an applicable quality metric for pediatric surgical care.CME jamanetwork.com/learning
Bortezomib for Neuromyelitis Optica Spectrum Disorder
Authors: Su-Hyun Kim
Journal: JAMA Neurology (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Neurology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Neurology journal
Pathologia et Microbiologia, Basel
Journal: JAMA (1968)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
STUDIES ON THE BACTERIOPHAGE OF D'HERELLE
Authors: J. Bronfenbrenner, R. S. Muckenfuss, C. Korb
Journal: The Journal of Experimental Medicine (1927)

In VoL xliv, 5, page 614, the third column in Section A of Protocol V should show the death of all of the mice. Two died within 24 hours, two within 48 hours, and the last within 72 hours. The appended diagram giving the correction for this column can be pasted in place on the original protocol. See PDF for Structure
THE PANCREAS IN DIABETES.
Journal: JAMA (1902)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The approach of a new millennium
Authors: L. S. King
Journal: JAMA (1990)

After one brief decade, we enter into a new millennium, whose approach exerts a certain fascination. If nothing else, we face imminent changes in some ingrained habits—it will indeed seem strange to date a letter or a check with the year 2000 (while remembering, of course, that the new millennium will really start only in 2001). However, will a transition from one millennium to another presage more important changes? The recent past offers some interesting data that on a smaller scale have considerable relevance. For example, the last decade of the 19th century, from about 1890 to 1900, witnessed such a major cultural transformation that it received a special name—the "mauve decade." Quite commonly it was called<i>fin de siècle</i>, "the end of a century," as if that provided an explanation. If we go back another 100 years to the end of the 18th century, we again find intense disturbance.
The provision and financing of medical care for AIDS patients in US public and private teaching hospitals
Authors: D. P. Andrulis
Journal: JAMA (1987)

The National Association of Public Hospitals and the Association of American Medical Colleges' Council of Teaching Hospitals conducted a detailed survey on hospital care to patients with acquired immunodeficiency syndrome (AIDS) in major US public and private teaching institutions in 1985. The 169 hospitals treating patients with AIDS that responded to the survey reported providing inpatient services to 5393 patients with AIDS. These patients accounted for 171205 inpatient days and 8806 inpatient admissions, with an average length of stay of 19 days. The average costs and revenue for patients with AIDS per day were $635 and $482, respectively, with Medicaid representing the most frequent third-party payer. The average inpatient cost per patient per year was $20 320. Using Centers for Disease Control estimates of 18 720 patients diagnosed as having AIDS and alive during any part of 1985, we estimate that the total cost of inpatient care for patients with AIDS was $380 million for that year. We also found significant regional and ownership differences in source of payment for patients with AIDS and regional differences in revenues received for AIDS treatment. Results indicate that the costs of treating patients with AIDS will profoundly affect major public and private teaching institutions, but that public teaching hospitals in states with restrictive Medicaid programs will be most adversely affected. (<i>JAMA</i>1987;258:1343-1346)
AB0999 Development of An Activity Disease Score in Patients with Uveitis (UVEDAI)
Authors: E. Pato, A. Martin-Martinez, A. Castellό, R. Méndez-Fernandez, S. Muñoz-Fernández, M. Cordero-Coma, L. Martinez-Costa, E. Valls, M. Reyes, F. Francisco, M. Esteban, A. Fonollosa, F. Sanchez-Alonso, C. Fernandez-Espartero, T. Diaz-Valle, J.M. Carrasco, E. Beltran-Catalan, M. Hernandez-Garfella, V. Hernandez, L. Pelegrin, R. Blanco, D. Diaz-Valle
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Uveitis is defined as inflammation of the uvea. As standardized and validated outcome measures are lacking in uveitis management. <h3>Objectives</h3> To develop a disease activity score for patients with uveitis and determine its discriminating ability to classify patients based on the level of activity. <h3>Methods</h3> Multicenter cross-sectional observational study. The construct "uveitis inflammation activity" was defined as intraocular inflammation. Seven dimensions and 14 items were identified and were agreed upon by experts via a two-round Delphi technique. Patients diagnosed with uveitis, who were aged five years or older and who presented inflammatory activity at the time of their visit, were selected. An ordinal logistic regression model was built in which the dependent variable was uveitis inflammatory activity (mild, moderate, or severe). Modeling accuracy for patient classification was assessed by the Area Under the Curve (AUC). Two AUC measures were calculated: one for discriminating mild versus moderate and severe activity level of uveitis, and another for mild and moderate versus severe activity. <h3>Results</h3> 195 patients were included. The regression model revealed an associations between intraocular activity level and an elevated number of anterior chamber cells (OR<sub>4 cells vs 0 cells</sub> = 27.85; 95% CI: 3.42; 226.75), a high degree of vitreous haze (OR<sub>severe vs null</sub> = 3.95; 95% CI: 1.40; 11.16), macular edema over 315mm (OR<sub>&gt;315 vs ≤315</sub> = 3.58; 95% CI: 1.56; 8.21), inflammatory vessel sheathing (OR=4.43; 95% CI: 1.71; 11.53), higher patient evaluation scores (OR<sub>1-unit increase</sub> = 1.23; 95% CI: 1.09; 1.39), the presence of papilitis (OR=4.05; 95% CI: 1.18; 13.92) and increased numbers of choroidal or retinal lesions (OR<sub>≥6 vs 0</sub> =4.99; 95% CI =1.08; 23.01). The discriminatory capacity of UVEDAI was 87% for differentiating patients with mild uveitis from those with moderate or severe uveitis, and 88% for discriminating patients with mild or moderate uveitis versus those with severe uveitis. <h3>Conclusions</h3> UVEDAI assesses and classifies patients depending on global ocular inflammatory activity with high discriminative capacity. <h3>Disclosure of Interest</h3> None declared
SUCCESSFUL THERAPEUTIC HYPOTHERMIA IN PATIENTS WITH HYPERTROPHIC CARDIOMYOPATHY
Authors: Barry J. Maron, Bradley A. Maron, Martin S. Maron, Tammy S. Haas, Robert K. Altman, Stephen J. Smalley, Joseph J. Doerer, Mark S. Link, Michael R. Mooney
Journal: Journal of the American College of Cardiology (2011)