tbuckley/cabot-search huggingface dataset:
P022/O02 Distinct macrophage phenotype and bioenergetic profiles in rheumatoid arthritis
Authors: MM Hanlon, T McGarry, M Canavan, C Low, DJ Veale, U Fearon
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Career situation of first and presenting author</h3> Student for a master or a PhD. <h3>Introduction</h3> Synovial macrophages play a key role in RA disease progression, however, the diversity and plasticity of macrophage subsets and their metabolic profile within the joint has yet to be elucidated. <h3>Objectives</h3> To phenotype distinct macrophage subsets within the RA joint, and determine the metabolic and inflammatory function of RA macrophages compared to healthy controls (HC). <h3>Methods</h3> Synovial-tissue biopsies from RA, PsA and OA, obtained through arthroscopy, were digested to yield a synovial single cell-suspension. Biopsy suspensions and synovial fluid mononuclear cells were analysed using advanced flow-cytometry with the following antibody panel (CD40,CD45,CD64,CD68,CD163,CD206,CD253). CD14+ monocytes were sorted from RA and HC bloods and differentiated/polarized into M1/M2 macrophages. Inflammatory (IL-8,-MCP-1,-IL1b,-CCR5,-IRAK1,-OSM) and metabolic (HIF1a,-PFKFB3,-PKM2,-LDHA,-HK2) markers were measured by RT-PCR, and phagocytosis by OVA luciferase-yellow assays. Glycolysis (ECAR) and oxidative phosphorylation (OCR) were quantified by Seahorse -XFE- technology. <h3>Results</h3> RA synovial-tissue and fluid CD68+ macrophages displayed markers typical of both M1(CD40+CD253+) and M2(CD206+CD163+). A significant increase in frequency of CD68+ and CD64+ macrophages in synovial-tissue compared to fluid was observed (p&lt;0.05), with significant increases in marker expression of CD40,CD163,CD206 (p&lt;0.07). A spectrum of macrophage-subtypes within the inflamed joint was observed, with significant enrichment of a dominant double positive CD206+CD163+ macrophage subtype in the synovial-tissue versus synovial fluid demonstrated (p&lt;0.05). Increased frequency of CD206+CD163+ macrophages and higher expression of activation marker CD40 were demonstrated in RA synovial-tissue compared to PsA and OA. M1 macrophages demonstrate a pro-glycolytic phenotype with significant increases in HIF1a,-HK2,-PKM2,-and PFKB3, compared to M2, effects exacerbated in RA macrophages compared to HC. In parallel, using seahorse-technology RA M1 and M2 macrophages displayed higher ECAR and OCR profiles, in addition to an increased ECAR:OCR ratio compared to HC (p&lt;0.05), evidence that RA macrophages switch to a glycolytic profile. This was paralleled by increased intracellular-cytokines expression of IL-1b,-IL-6 and TNFa and genes expression of IL-8,-IL1b, OSM and MCP-1 (p&lt;0.05). Finally, phagocytic ability of RA M1 was impaired compared to HC. <h3>Conclusions</h3> We have identified, for the first time, a dominant macrophages subtype enriched in RA synovial-tissue. Furthermore, RA M1/M2 have distinct metabolic profiles associated with differences in key inflammatory mediator and phagocytosis function. <h3>Disclosure of Interest</h3> None declared.
“Rapid diagnosis of primary ciliary dyskinesia: cell culture and soft computing analysis.” Massimo Pifferi, Andrew Bush, Francesca Montemurro, Giovanni Pioggia, Martina Piras, Gennaro Tartarisco, Maria Di Cicco, Iolanda Chinellato, Angela M. Cangiotti and Attilio L. Boner.<i>Eur Respir J</i>2013; 41: 960–965.
Journal: European Respiratory Journal (2013)
Abstract 481: Distinct and overlapping patterns of B-cell growth pathway mutations in CD5-negative B-cell lymphoproliferative disorders
Authors: Sophia Shaddy, Weiqiang Zhao, Huolin Tu, Brianna Sisson, Rongqin Ren, Sean Caruthers, Susan Long, Peng Ru, Narendranath Epperla, Lynne Abruzzo, Kami Maddocks, Dan Jones
Journal: Cancer Research (2019)

Abstract Low-grade CD5-negative B-cell lymphoproliferative disorders (CD5- LG B-LPD) encompass several entities that often show mutations in different growth regulatory pathways. These include MYD88 (TLR signaling), CD79A/B (BCR complex), CARD11 (CBM signalosome), CXCR4 (chemokine receptor), and NOTCH1/2 and are complemented by KLF2 mutations and BCL6 and MALT1 overexpression. Using targeted, next-generation sequencing (NGS) for 50 B-cell associated growth regulators and epigenetic modifiers (&amp;gt;1000X depth) and cytogenetic/FISH data, we sought to delineate the complementary mutation and gene amplification patterns in 45 consecutive cases of CD5- LG B-LPD. In mutation-negative cases, a 572-gene NGS panel was used to interrogate these pathways more completely. WHO diagnostic criteria were used, without reliance on molecular data, emphasizing morphology features, paraprotein level and type to distinguish lymphoplasmacytic lymphoma (LPL) from marginal zone lymphoma (MZL). As expected, MYD88 L265P mutations were seen in the majority of LPL (11/17, 65%), but also in CD5- chronic lymphocytic leukemia (CLL) (3/9, 33%) and splenic (S) MZL (1/6, 17%) but not in nodal MZL. In 4 LPL cases lacking MYD88 L265P, extra copies of chromosome 3 (BCL6), 6 (IRF4), 12 and/or 18 (MALT1) were seen, with CARD11 mutations in the other 2. NOTCH1/2 mutations were mostly associated with SLL/CLL (3/9, 33%). Mutations in epigenetic regulators (see Table) were seen in the majority of nodal MZL (8/13, 62%) but only few LPL (2/17, 12%) and SMZL (1/6, 17%). CXCR4 inactivating mutations were seen with MYD88 and CD79B mutations and +3/BCL6 alteration. CARD11 mutations were seen with KLF2, MYD88 and NOTCH1/2 mutations. Less common mutations targets included PTPRC and PLCG2 (pre-ibrutinib treatment). The higher rate of mutation in epigenetic regulators (along with loss of KLF2-mediated BCR signaling) supports a distinct pathogenesis for nodal MZL in contrast to other CD5- B-LPD. Calls per gene in MYD88 negative cases; *TP53, TET2, ASXL1, DNMT3A, BCORCD79BCARD11NOTCH1/2CXCR4KLF2Epigenetic gene set*LPL023000010000000010CD5- CLL000002001001001000001000000001sMZL011010010000000001nMZL000002000022000001010011000101000001000001000001012010000200 Citation Format: Sophia Shaddy, Weiqiang Zhao, Huolin Tu, Brianna Sisson, Rongqin Ren, Sean Caruthers, Susan Long, Peng Ru, Narendranath Epperla, Lynne Abruzzo, Kami Maddocks, Dan Jones. Distinct and overlapping patterns of B-cell growth pathway mutations in CD5-negative B-cell lymphoproliferative disorders [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 481.
Closed irrigation-suction of spine infections. A review of four successfully treated cases of pyogenic vertebral osteomyelitis
Authors: E. O. Leventen
Journal: JAMA (1966)

A relatively simple technique is adapted for closed suction-irrigation with antibiotic and detergent as treatment of chronic osteomyelitis of the spine. The antibiotic used is one known to be effective against the infecting organism. The irrigation is continued until two consecutive negative cultures of suction fluid are obtained. This is usually a week to ten days. Of four successfully treated patients, one had been draining continuously for six years and the others for about six months.
Dynamic DNA methylation change in the CpG island region of p15 during human myeloid development
Authors: Kazuo Sakashita, Kenichi Koike, Tatsuya Kinoshita, Masaaki Shiohara, Takehiko Kamijo, Shun’ichiro Taniguchi, Takeo Kubota
Journal: Journal of Clinical Investigation (2001)

We examined the kenetics of p15 methylation and expression during myeloid development. We treated human cord blood CD34+ cells with either GM-CSF alone or in combination with stem cell factor and followed methylation at this locus using bisulfite genomic sequencing. CD34+ cells were found to be either fully methylated or completely unmethylated at 27 CpG dinucleotide sites in exon 1 and at 18 CpG sites in the promoter region of the p15 gene. A time-course study showed that the percentage of the allelic methylation of p15 CpG island increased to approximately 50% to 60% until 7 days after cytokine stimulation, then decreased to less than 10% after 21 days. The methylation was also observed in bone marrow CD34+ cells exposed to GM-CSF. p15 expression varied inversely with methylation. Expression was negligible or at low levels until 14 days, after which it increased substantially. The frequency of myeloid colony-forming cells in the progeny decreased and myeloid-specific markers increased in the later stages. Based on our observations on cells grown with GM-CSF and 5-aza-2′-deoxycytidine, DNA methylation of the p15 promoter region CpG island appears to be associated with proliferation rather than differentiation of normal human myeloid progenitors.
What's new in the other general journals
Authors: Alison Tonks
Journal: BMJ (2006)

Age related macular degeneration is an important cause of blindness among older people, particularly in developed countries. The aetiology is still unclear, but a new study suggests that about half of all cases are caused by polymorphism at the gene coding for complement factor H, an important inflammatory mediator that inhibits the complement cascade. Credit: ANN INTERN MED In a cohort of people aged over 55 from Rotterdam in the Netherlands, carriers of the Y402H allele were significantly more likely than non-carriers to develop age related macular degeneration over a median follow-up of 10 years. They were also more likely to get worse. People homozygous for the allele had an 11-fold increase in risk of sight threatening degeneration (odds ratio 11.02; 95% CI 6.82 to 11.81), relative to non-carriers. Overall, 36.2% (4116/11 362) of the cohort carried at least one allele. Genetic predisposition became more important after the age of 75, and the authors estimate that nearly half the homozygotes in their cohort would develop a sight threatening form of the disease by the age of 95. Smoking is a well known risk factor for age related macular degeneration, but in this study it was particularly risky for people with an existing genetic predisposition. Homozygotes for the Y402H allele were over 30 times more likely to develop serious age related macular degeneration than non-carriers who did not smoke (odds ratio 34.0; 13.0 to 88.6). Credit: JAMA JAMA 2006; 296: 301-9 [OpenUrl][1][CrossRef][2][PubMed][3][Web of Science][4] Being overweight or obese at age 18 increases your chances of a premature death, according to a cohort study of over 100 000 American nurses. Even nurses who remembered being only moderately overweight at 18 had an increased risk of dying before late middle age (hazard ratio 1.66; 95% CI 1.31 to 2.10 for a body mass index of 25.0 to 29.9 relative to … [1]: {openurl}?query=rft.jtitle%253DJAMA%26rft.stitle%253DJAMA%26rft.issn%253D0002-9955%26rft.aulast%253DDespriet%26rft.auinit1%253DD.%2BD.%2BG.%26rft.volume%253D296%26rft.issue%253D3%26rft.spage%253D301%26rft.epage%253D309%26rft.atitle%253DComplement%2Bfactor%2BH%2Bpolymorphism%252C%2Bcomplement%2Bactivators%252C%2Band%2Brisk%2Bof%2Bage-related%2Bmacular%2Bdegeneration.%26rft_id%253Dinfo%253Adoi%252F10.1001%252Fjama.296.3.301%26rft_id%253Dinfo%253Apmid%252F16849663%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [2]: /lookup/external-ref?access_num=10.1001/jama.296.3.301&link_type=DOI [3]: /lookup/external-ref?access_num=16849663&link_type=MED&atom=%2Fbmj%2F333%2F7561%2F249.atom [4]: /lookup/external-ref?access_num=000239089900022&link_type=ISI
Bells
Authors: Cinnamon Bradley
Journal: JAMA (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Peroral cholangioscopy after operations on the vater's papilla Medical Center, ?Aeroflot Russian International Airlines?, Moscow
Journal: Hepatology (1995)

Recurrent pyogenic cholangitis (RPC) is characterized by repeated attacks of bacterial infection of the biliary tract by enteric organisms resulting in formation of strictures and stones in the intrahepatic as well as the extrahepatic bile ducts. Between 1973 and 1984, we managed ten children with RPC. Presentation was acute, septicemia being invariably present. Four children responded to vigorous intravenous (IV) fluid and antibiotic therapy; of these, three subsequently required elective transduodenal sphincteroplasty. Six children required emergency biliary tract decompression surgically and three of them also had concomitant definitive drainage procedures (transduodenal sphincteroplasty, two; supraduodenal choledochoduodenostomy, one). The other three had T-tube drainage initially followed by elective drainage procedures (transduodenal sphincteroplasty, one; supraduodenal choledochoduodenostomy, two). At exploration, pigment stones and/or mud were invariably present in the bile ducts. One child required reexploration for hemostasis following sphincteroplasty, and one child died from septicemia and respiratory failure following operation. The rest are well, having been followed for 3 to 12 years. Major advances in recent years include (1) endoscopic retrograde cholangiopancreatography (ERCP) for accurate definition of the biliary tract and confirmation of the presence and location of stones during the quiescent phase, (2) the intraoperative choledochoscopic extraction of intrahepatic and extrahepatic stones, and (3) postoperative stone removal via the T-tube tract.
Breast Imaging
Authors: Daniel B. Kopans, Jack E. Meyer, Norman Sadowsky
Journal: New England Journal of Medicine (1984)

THE initial enthusiasm for breast-cancer screening, stimulated by the successful use of x-ray mammography in detecting early-stage breast cancer, was diminished in 1976 when concerns were raised about the theoretical potential for x-ray carcinogenesis.1 Since that time, various methods have been developed for imaging the breast, in an effort to duplicate the accuracy of mammography without the use of x-rays. At the same time, technological advances in mammography have resulted in a marked reduction of the radiation dose and improved image quality. Nevertheless, the majority of the 115,000 new cases that will be diagnosed in 1984 will occur in women . . .
Reply:
Authors: Eric M. Gershwin
Journal: Hepatology (2008)

Unless we are both studying the same sera, there is no basis of comparison. In our clinical cases and I assume their clinical series, many types of ALF were studied and we both found AMAs when we used the same method. It is also obvious that no international standardization exists, and measurements of all these antibodies will not be standardized until the release of the newer multiphasic kits in 2009 and beyond. M. Eric Gershwin M.D.*, * Division of Rheumatology/Allergy, University of California, Davis Medical Center, Sacramento, CA.
Editorial Board
Authors: Donald Leung, Stanley Szefler, Colo Associate, Cezmi Akdis, Davos Switzerland, Andrea Apter, Pedro Philadelphia, Chicago Avila, Bruce Bochner, Joshua Boyce, David Broide, M Chb, La Jolla, Robert Calif, Madison Bush, Fred Finkelman, Dennis Ledford, David Peden, Marc Rothenberg, Cincinnati Ohio, William Shearer, Scott Sicherer, New York, Donata Vercelli, Ariz Statistics Editor, Vernon Chinchilli
Journal: Journal of Allergy and Clinical Immunology (2010)
"STILBAMIDINE" THERAPY
Authors: Joseph M. Miller
Journal: JAMA (1953)

<h3>To the Editor:</h3> —The comment of Cohen on the dangers of "stilbamidine" therapy inThe Journal(page 1332, Nov. 29, 1952) reiterates a warning that has been made in the literature on numerous occasions. These references may be found in an article on blastomycosis and its treatment with "stilbamidine," by Schoenbach, Miller, and Long (<i>Ann. Int. Med.</i><b>37:</b>31-47 [July] 1952). Exposure of "stilbamidine" to light for short periods of time will produce a toxic product. The end products of such deterioration are not known with certainty. Evidence of hepatic or renal toxicity following treatment in man with fresh solutions has not been observed. Several thousand patients, including many who were considered poor risks, chronically ill Asiatics and Africans, have been treated with one or more of the diamidines. Deaths due to delayed poisoning with hepatic and renal injury have been reported in patients who were given solutions made in
Section 7: Rheumatoid Arthritis
Journal: JAMA (1973)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Gastroenterology and Hepatology
Authors: J. Donald Ostrow
Journal: JAMA (1985)

Although histamine H<sub>2</sub>-receptor blockers remain the mainstay of therapy for peptic ulcer, the experimental agent omeprazole has proved to be the most potent acid suppressant. Administered once daily, it may suppress gastric acid secretion for up to three days. Clinical trials in Zollinger-Ellison syndrome support the advantage of omeprazole over H<sub>2</sub>-receptor—antagonist therapy.<sup>1</sup>Omeprazole, when compared in a double-blind fashion to cimetidine, has been shown to heal duodenal ulcers more rapidly.<sub>2</sub>Limiting enthusiasm for this agent, however, is the development of carcinoid tumors in some rats treated with omeprazole for two years, a finding that led to temporary suspension of European clinical trials.<sup>3</sup> Development of gastric carcinoid in 8% of a large group of patients with pernicious anemia, as well as reports of malignant carcinoid complicating the Zollinger-Ellison syndrome, suggests that chronic hypersecretion of gastrin, whether induced by suppression of acid secretion or by
LAW AND MEDICINE
Authors: Richard P. Bergen
Journal: JAMA (1967)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Prenatal Exposure of the Northern Quebec Inuit Infants to Environmental Contaminants
Authors: Gina Muckle, Pierre Ayotte, Eric Dewailly, Sandra W. Jacobson, Joseph L. Jacobson
Journal: Environmental Health Perspectives (2001)

Mercury is a heavy metal that enters the environment from both natural and anthropogenic sources.A natural element in the earth's crust, Hg is converted by bacteria to methylmercury (MeHg) in lakes and oceans and bioaccumulates in the marine food web.Hg is also released into the environment through human activities, mainly burning of fossil fuels and waste incineration.Polychlorinated biphenyls (PCBs) and chlorinated pesticides are organochlorine compounds (OCs), a family of lipophilic compounds used extensively in North American and European industry and agriculture from 1930 to the mid-1980s (1,2).OCs have been dispersed widely in the environment.Even in industrialized countries where they are no longer being manufactured, they are still detectable in the environment because they resist degradation, are released into the environment by inadvertent spills and careless disposal, and in some countries are still in use (3).The PCBs used for industrial purposes were complex mixtures of various congeners, each with its own unique molecular structure and potentially different toxicological effects.
Influence of nutritional status and posttransplant nutrition support on morbidity and mortality rates following liver transplantation JM Hasse, LS Blue, JS Crippin, RM Goldstein, LW Jennings, TA Gonwa, BS Husberg, MF Levy, GB Klintmalm. Transplantation Services; Baylor University Medical Center; Dallas, TX
Journal: Hepatology (1993)
Routine Contraception
Authors: Karl Kautsky
Journal: JAMA (1961)

<h3>To the Editor:—</h3> In<i>Medical World News</i>Aug. 26, 1960, is notice of a project of the Planned Parenthood Federation to use Enovid tablets as a "routinely" taken contraceptive. As former chief of the Municipal Premarital and Marital Guidance Clinic of Vienna (1921-1934), I take exception, not to the purpose, but, to this method of hormonal contraception. The persistent ingestion of progestin-like hormones works by suppression of ovulation. This might be harmless if used for a short while, but much caution should be taken if used for a long time. Most females in German concentration camps lost their menstrual periods, sometimes for years; usually immediately after their detainment, as a reaction to the fear caused by the Nazi terror.<sup>1</sup>After their release from these camps most of the younger inmates started ovulating and menstruating again, and later became pregnant; but there was a startling after effect, as work by