tbuckley/cabot-search huggingface dataset:
Abstract 166: MMP12 promotes EMT-mediated tumor aggressiveness in lung cancer cells
Authors: Na-Kyung Han, Yoon-Jin Lee, Hae-June Lee
Journal: Cancer Research (2019)

Abstract Epithelial mesenchymal transition (EMT) plays a pivotal role in cancer progression and metastasis. Matrix metalloproteinases (MMPs) have a major role in remodeling the extracellular matrix (ECM) and are implicated in the invasion and metastasis of various malignancies. MMP12 is involved in many pathological processes including cancer, but has not been fully understood yet. To investigate the role of MMP12 in lung cancer cells, we examined the effect of overexpression or knockdown of MMP12 in A549 cells and measured the protein expression level of EMT markers. MMP12 overexpression induced considerable EMT phenotypic changes compared to the control group, both in vitro and in vivo. The mRNA levels of EMT markers, snail2/slug, snail1, zeb1, TGFβ-1, and fibronectin, increased significantly upon MMP12 overexpression. We observed that changes in the expression level of ECM components such as, collagen, lamin, and proteoglycans led to MMP12 overexpression-induced EMT. These alterations promoted the aggressiveness of the A549 cells, evident from changes in functional properties of the cell, such as proliferation, migration, invasion, and adhesion capacity. On the contrary, MMP12 suppression abrogated malignancy of A549 cells. MMP12 knockdown in A549 cells led to decreased mRNA expression of EMT marker proteins associated with adherence, invasion, and migration. Our results suggest that MMP12 is a key regulator of EMT in non-small cell lung cancer (NSCLC). MMP12 overexpression in A549 cells results in changes in the expression of EMT markers and ECM composition. Interestingly, the dynamic changes in the ECM components by MMP12 overexpression cause EMT reprogramming and induce malignant functional properties. Thus, we suggest that the control of MMP12 expression can be a potential target for new therapeutic strategies of NSCLC. Citation Format: Na-Kyung Han, Yoon-Jin Lee, Hae-June Lee. MMP12 promotes EMT-mediated tumor aggressiveness in lung cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 166.
OPERATIVE TREATMENT FOR TYPHOID PERFORATION.
Journal: JAMA (1899)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
ANTIPROPRIETARY DRUG LEGISLATION.
Journal: JAMA (1899)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Behavior Control
Authors: Frank J. Ayd
Journal: JAMA (1970)

This fascinating book is a comprehensive account of the growing technology of behavior control. The data Dr. London has assembled and put in context will surprise many people, including most of the scientists, technicians, teachers, doctors, engineers, and other specialists in the learned professions, many of whom have not yet looked upon the tools that they use as parts of a technology for controlling behavior. Dr. London leaves no doubt about the extent to which it is now possible to manipulate people systematically. And, he rightfully labels these as "petty compared to what will soon be possible. Means are being found, in all the crafts and sciences of man, society, and life, that will soon make possible precise control over much of people's individual actions, thoughts, emotions, moods, and wills." This enlightening and thoughtprovoking volume is composed of three parts. Dr. London begins by discussing "The Advent of Behavioral Control"
STUDIES IN GYNECOLOGY FROM THE SERVICE OF THE WOMAN'S HOSPITAL OF PHILADELPHIA.
Authors: ANNA M. FULLERTON
Journal: JAMA (1897)

My paper does not pretend to be a contribution to the most interesting and valuable subject of surgical gynecology but rather a plea for what may be termed preventive gynecology, to which perhaps we have given too little heed in the past. Not that I would in any way decry the brilliant and skilful results attained by surgical gynecology in this day, for it is too true that diseased conditions affecting the pelvic viscera very frequently result in the production of organic lesions which induce chronic invalidism and render the functional activity of the generative organs a menace to the health and even the life of the patient. Too often, however, the operator himself realizes that in the removal of diseased pelvic organs he is but cutting away, as it were, dead twigs and branches from a plant which has a worm at the root. It is not a matter
Essai randomisé évaluant l’efficacité et la tolérance de l’ablation par radiofréquence des cholangiocarcinomes extra-hépatiques non résécables
Journal: Endoscopy (2018)

La mise en place endoscopique d’une prothèse biliaire pour soulager l’ictère est déterminante dans le traitement palliatif des cholangiocarcinomes extra-hépatiques non résécables. Il a été montré dans des travaux de faible niveau de preuve que l’ablation par radiofréquence (RFA) endobiliaire pouvait prolonger la perméabilité des prothèses et ainsi améliorer la survie des malades.
Manuscripts Approved for JNCI During December 1975-January 1976
Journal: JNCI Journal of the National Cancer Institute (1976)

Journal Article Manuscripts Approved for JNCI During December 1975-January 1976 Get access JNCI: Journal of the National Cancer Institute, Volume 56, Issue 2, February 1976, Pages 453–455, https://doi.org/10.1093/jnci/56.2.453-d Published: 01 February 1976
Die Klinik der Nierenkrankheiten. Kurs-Vorlesungen für praktische Ärzte und Studierende.
Journal: JAMA (1927)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Index of Subjects&lt;subtitle&gt;Volume 27 July 1961 • December 1961&lt;/subtitle&gt;
Journal: JNCI Journal of the National Cancer Institute (1961)

Journal Article Index of Subjects: Volume 27 July 1961 • December 1961 Get access JNCI: Journal of the National Cancer Institute, Volume 27, Issue 6, December 1961, Pages 1521–1528, https://doi.org/10.1093/jnci/27.6.1521 Published: 01 December 1961
AB0161 Identification and validation of a protein combination including s100a9 able to predict the response to the mtx/etanercept association in rheumatoid arthritis patients
Authors: A. Obry, P. Cosette, J. Hardouin, O. Boyer, O. Vittecoq, T. Lequerré
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The number of biologic agents in Rheumatoid Arthritis (RA) is continuously increasing. However, clinicians observe that around 30 to 40% of treated patients fail to respond to TNFα blocking agents. One way to optimize the drug prescription is to identify predictive markers of drug responsiveness. <h3>Objectives</h3> To identify a combination of serum proteins whose expression profile would predict the RA patients responses to the association of methotrexate (MTX) and etanercept (ETA) by mass spectrometry-based quantification methods and ELISA. <h3>Methods</h3> A "cohort discovery phase" of 22 patients with active RA was treated by a subcutaneous injection of ETA. The clinical efficacy was evaluated with the DAS28 score after 6 months of treatment according to the EULAR response criteria. For proteomic analysis, a serum sample was collected in patients prior to treatment exposure. A "label free" approach on the whole proteome was performed by mass spectrometry on the 22 sera. Accordingly, the proteome of each sample was extracted and in-gel digested. The resulting peptides were analyzed by LTQ Orbitrap® (ThermoFisher). Differential analysis between responder and non responder samples was performed with LCMS ProGenesis® (Nonlinear Dynamics). To validate these results a relative quantification of selected protein was performed on the second "cohort validation phase" by ELISA. The proteome of peripheral blood mononuclear cells (PBMC) from a second cohort of seven patients with similar characteristics has also been studied by the same label free approach. <h3>Results</h3> The label free approach revealed 12 differentially expressed serum proteins according to patient response. This combination of proteins was used to build a Random Forest statistical model to predict the patient's status. This model was validated by a blind test on a panel of seven patients. Moreover, these results have shown the protein S100A9 overexpression in both the serum and the PBMCs from responder's patients and this expression was confirmed by ELISA <h3>Conclusions</h3> The label free approach has identified a combination of predictive markers of response to MTX treatment / ETA. Thus, using sera samples collected in patients prior to treatment exposure, it is possible to predict response to treatment with a small error. These proteins represent interesting candidate biomarkers of response that must be validated in a larger population. Already identified as a diagnostic and prognostic biomarker of RA, the S100A9 protein has been identified as a predictive biomarker of response both in serum and in PBMCs. <h3>Disclosure of Interest</h3> None Declared
BOOK REVIEWS
Authors: R. F. AMBINDER
Journal: JNCI Journal of the National Cancer Institute (1991)

BOOK REVIEWS Get access New Aspects in the Diagnosis and Treatment of Hodgkin's Disease: Recent Results in Cancer Research, Volume 117. V. Diehl M. Pfreundschuh , M. Loeffler , eds. Heidelberg : Springer-Verlag , 1989 , 283 pp. $119. RICHARD F. AMBINDER RICHARD F. AMBINDER The Johns Hopkins Hospital Oncology Center 418 N. Bond St.Baltimore, MD 21231 Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 83, Issue 16, 21 August 1991, Pages 1176–1177, https://doi.org/10.1093/jnci/83.16.1176-a Published: 21 August 1991
Abstract 14192: Stroke Recurrence and Atrial Fibrillation Occurrence in the Patients With Right Insular Stroke
Authors: Hee-Kwon Park, Sang-Bae Ko, Joung-Ho Rha
Journal: Circulation (2015)

Introduction: The right insular cortical lesion is believed to have the arrhythmogenic potential such as secondary atrial fibrillation(AF). The P wave-triggered signal-averaged electrocardiogram(SA-ECG) can detect the P wave dispersion which is related to the risk of AF. The stroke risk of secondary AF after right insular stroke remained unclear. Hypothesis: We investigated the arrhythmogenic potential of right insular stroke, using SA-ECG and the stroke recurrence rate related to stroke location. Methods: We recruited acute stroke patients who admitted from February 2012 to October 2013 and took routine work-up, including SA-ECG. Patients who had the previous history of AF before admission, were excluded. We checked the stroke recurrence and AF occurrence over two years after index stroke. We analyzed the difference of SA-ECG, AF occurrence and stroke recurrence rate between the right insular cortex lesion and other stroke. Results: A total of 252 subjects were enrolled and 49 among them had right insular involvement. The patients with right insular lesion had longer P wave duration than the other stroke patients (154.0+29.6 vs. 133.5+26.5 ms, p&lt;0.001). During observation period, stroke recurred in 28 patients and AF occurred in 50 persons. The patients with right insular lesion suffered from the AF occurrence and the stroke recurrence more frequently than those with other stroke lesions(AF occurrence, 33% vs 17%, P=0.01; Stroke Recurrence 25% vs 6%, P&lt;0.01). Among the patients with AF occurrence, the patients with right insular lesion had the tendency of the higher stroke recurrence rate than those with the other stroke lesion(43.8% vs 20.6%, P=0.089). Conclusions: Our result suggests that the right insular lesion is related to increased P wave dispersion and this might explain the development of secondary AF after right insular cortex stroke. Secondary AF after right insular lesion might have the higher stroke risk than that after other stroke lesion.
Protein kinase C-η regulates resistance to UV- and γ-irradiation-induced apoptosis in glioblastoma cells by preventing caspase-9 activation
Authors: Isa M. Hussaini, Joan E. Carpenter, Gerard T. Redpath, Julianne J. Sando, Mark E. Shaffrey, Scott R. Vandenberg
Journal: Neuro-Oncology (2002)

Neuro-OncologyBoth increased cell proliferation and apoptosis play important roles in the malignant growth of glioblastomas.We have demonstrated recently that the differential expression of protein kinase C (PKC)-h increases the proliferative capacity of glioblastoma cells in culture; however, speci c functions for this novel PKC isozyme in the regulation of apoptosis in these tumors has not been de ned.In the present study of several glioblastoma cell lines, we investigated the role of PKC-h in preventing UVand g-irradiation-induced apoptosis and in caspasedependent signaling pathways that mediate cell death. Exposure to UV or g irradiation killed 80% to 100% of PKC-h-de cient nonneoplastic human astrocytes and U-1242 MG cells, but had little effect on the PKC-h-expressing U-251 MG and U-373 MG cells. PKC-h appears to mediate resistance to irradiation specifically such that when PKC-h was stably expressed in U-1242 MG cells, more than 80% of these cells developed resistance to irradiation-induced apoptosis. Reducing PKC-h expression by transient and stable expression of antisense PKC-h in wild-type U-251 MG cells results inProtein kinase C-h regulates resistance to UV-and g-irradiation-induced apoptosis in glioblastoma cells by preventing caspase-9 activation
Phospholamban gene ablation improves calcium transients but not cardiac function in a heart failure model
Authors: A JANCZEWSKI
Journal: Cardiovascular Research (2004)

Decreased amplitude and slower kinetics of cardiomyocyte intracellular calcium (Cai2+) transients may underlie the diminished cardiac function observed in heart failure. These alterations occur in humans and animals with heart failure, including the TNF1.6 mouse model, in which heart failure arises from cardiac-specific overexpression of tumor necrosis factor α (TNFα). Objective: Since ablation of phospholamban expression (PLBKO) removes inhibition of the sarcoplasmic reticulum (SR) Ca2+ pump, enhances SR Ca2+ uptake and increases contractility, we assessed whether ablation of phospholamban expression could improve cardiac function, limit remodeling, and improve survival in the TNF1.6 model of heart failure. Methods: We bred PLBKO with TNF1.6 mice and characterized the progeny for survival, cardiac function (echocardiography), cardiac remodeling (hypertrophy, dilation, fibrosis), and Cai2+ transients and contractile function of isolated cardiomyocytes. Results: PLB ablation did not improve survival, cardiac function, or limit cardiac chamber dilation and hypertrophy in TNF1.6 mice (TKO mice). However, contractile function and Cai2+ transients (amplitude and kinetics) of isolated TKO cardiomyocytes were markedly enhanced. This discordance between unimproved cardiac function, and enhanced Cai2+ cycling and cardiomyocyte contractile parameters may arise from a continued overexpression of collagen and decreased expression of gap junction proteins (connexin 43) in response to chronic TNFα stimulation. Conclusions: Enhancement of intrinsic cardiomyocyte Cai2+ cycling and contractile function may not be sufficient to overcome several parallel pathophysiologic processes present in the failing heart.
THE SO-CALLED MAGIC TOUCH AS OPPOSED TO LABORATORY PRECISION IN DIAGNOSIS.
Journal: JAMA (1906)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
529P Exploratory study for preventing nausea and vomiting by switching from pranisetron + dexamethasone (Days 1–3) + aprepitant (Days 1–3) to palonosetron + pexamethasone (Day 1) in patients undergoing moderately emetogenic chemotherapy
Authors: T. Kimura, K. Otsuka, M. Yaegashi, M. Hakozaki, T. Matuo, H. Fujii, K. Sato, M. Kamishima, T. Miyake, T. Takahara, Y. Akiyama, T. Iwaya, S. Nishizuka, H. Nitta, K. Koeda, M. Mizuno, Y. Kimura, A. Sasaki
Journal: Annals of Oncology (2016)
Relapsing Polychondritis
Authors: David E. Trentham
Journal: Annals of Internal Medicine (1998)

Relapsing polychondritis, an uncommon, chronic, multisystem disorder characterized by recurrent episodes of inflammation of cartilaginous tissues, can be life-threatening, debilitating, and difficult to diagnose. This review is based on the authors' experience with 36 patients with relapsing polychondritis who were followed from 1980 to 1997, 30 patients located elsewhere who completed a detailed questionnaire and interview, and a perusal of English-language textbooks and papers located by a systematic search of the MEDLINE database. Relapsing polychondritis can present in a highly ambiguous fashion; therefore, in the authors' series, the mean delay from the time medical attention was sought because of symptom onset until diagnosis was 2.9 years. Although prednisone was the main form of treatment, methotrexate seemed to be of additional value. Survival was much more favorable than previously thought. Greater awareness of relapsing polychondritis would probably lead to earlier diagnosis and better outcomes.
643. Human Matrix Metalloproteinase-8 Gene Delivery Enhances the Oncolytic Activity of a Replicating Adenovirus
Authors: Jin Cheng, Harald Sauthoff, Yao Qi Huang, David I. Kutler, Sheila Heitner, William N. Rom, John G. Hay
Journal: Molecular Therapy (2006)

The success of replicating adenoviruses in cancer therapy is limited by inefficient delivery. The suboptimal transduction of cancer cells is compounded by poor distribution of the virus within the tumor mass. The hypothesis that is addressed in this study is that matrix within the tumor hinders the free cell-to-cell spread of replicating adenoviral vectors. Collagen I, collagen IV, fibronectin and laminin matrix components were obtained commercially pre-coated on membrane inserts. The membrane inserts were placed into the chambers of 24-well plates that had been pre-seeded with 293 cells growing as a monolayer on the base. Adβ-gal virus was added to the upper chamber, and in order to infect the 293 cells plated on the base of the wells, the virus had to spread through the matrix pre-coated insert. The collagen I insert blocked the spread of Adβ-gal virus so that only a few 293 cells stained blue after the addition of X-gal. In contrast, control, collagen IV, fibronectin and laminin inserts allowed the passage of virus with subsequent intense blue staining of the 293 indicator cells. A significant reduction of viral passage through the Collagen 1 matrix was confirmed with assays of β-gal acivity. Based on reports of the effective collagen I-degrading activity of MMP-8, we chose to construct an non-replicating adenovirus to express the MMP-8 transgene. Established human A549 cell lung cancer xenografts were injected with a wild-type replicating adenovirus Adwt (5 × 108 PFU) together with the non-replicating AdMMP-8 virus (5 × 108 PFU). Histochemical analysis demonstrated reduced amounts of collagen within necrotic areas of Adwt plus AdMMP-8 injected tumors compared to controls. The addition of AdMMP-8 to the wild type virus also significantly reduced the growth of tumors compared with control tumors injected with the wild type virus in combination with the non-replicating control virus. AdMMP-8 injection alone did not affect the growth of the lung A549 tumor xenografts. These results suggest that intratumoral AdMMP-8 and collagen disruption is a possible strategy for improving viral spread and improving the oncolytic activity of replicating adenovirus.