tbuckley/cabot-search huggingface dataset:
Abstract 153: Using Enzyme-Catalyzed Proximity Labeling to Define the Interactome of the Insulin-like Growth Factor-1 Receptor
Authors: Akshay Bareja, Conrad Hodgkinson, Alan Payne, Erik Soderblom, Greg Waitt, Victor Dzau
Journal: Circulation Research (2017)

Introduction: Manipulation of I nsulin-like G rowth F actor- 1 R eceptor (IGF1R) signaling has been proposed to be an important therapeutic for the treatment of heart failure and myocardial infarction. However, activation of this receptor has, under certain conditions, been shown to result in deleterious side-effects such as pathological hypertrophy. Development of therapeutic strategies that avoid these side-effects requires a more comprehensive understanding of the proteins involved in the IGF1R signaling axis. To this end, we have made use of the enzyme-catalyzed proximity labeling technique called BioID. Objective: To comprehensively define the interactome of the activated IGF1R. Methods and Results: Briefly, BioID makes use of a biotin protein ligase called BirA* that promiscuously biotinylates neighboring proteins. The protein of interest is fused with BirA* and is then expressed in a particular cell line. Following administration of an excess amount of biotin, BirA* will biotinylate those proteins that are adjacent to or interact with the protein of interest. Interacting proteins are identified by tandem mass spectrometry (MS/MS). We have made a HEK 293T cell line that stably expresses the BirA* biotin ligase fused to the C-terminus of IGF1R. We performed BioID using this cell line (N=3) to identify a set of proteins that bind to the IGF1R following stimulation by IGF1. This set included many known interactors of the IGF1R, such as IRS2 and SHC1, thereby validating this technique. Of the many interesting novel interactors, we chose to pursue validation of sorting nexin 6 (SNX6) as some SNX proteins have been shown to be involved in receptor recycling to the plasma membrane following stimulation. We have found that SNX6 knockdown results in a dramatic diminution of IGF1-mediated ERK phosphorylation (2-fold; N=4; p &lt;0.05). However, there was no effect on IGF1-mediated Akt phosphorylation (N=4). We are currently assessing the role of SNX6 in IGF1R-mediated function and recycling of this receptor. Conclusion: We have demonstrated the ability of the BioID technique to identify a set of proteins that can interact with the activated IGF1R. Moreover, BioID has identified a number of novel interactors that further define IGF1R signaling and function.
Occurrence and Distribution of Arsenic in Soils and Plants
Authors: Leo M. Walsh, Malcolm E. Sumner, Dennis R. Keeney
Journal: Environmental Health Perspectives (1977)

Inorganic arsenicals have been used in agriculture as pesticides or defoliants for many years and, in localized areas, oxides of arsenic have contaminated soils as a result of fallout from ore-smelting operations and coal-fired power plants. Use of inorganic arsenicals is no longer permitted in most agricultural operations, and recent air pollution controls have markedly reduced contamination from smelters. Thus, this paper will concentrate on the effect of past applications on arsenic accumulation in soil, phytotoxicity to and uptake by plants as influenced by soil properties, and alleviation of the deleterious effects of arsenic. Once incorporated into the soil, inorganic arsenical pesticides and arsenic oxides revert to arsenates, except where the soil is under reducing conditions. The arsenate ion has properties similar to that of orthophosphate, and is readily sorbed by iron and aluminum components. This reaction greatly restricts the downward movement (leaching) of arsenic in soils and the availability of arsenic to plants. Several methods of estimating plant available arsenic in soils have been developed. They involve extraction of the soil with reagents used to estimate phosphorus availability. This extractable arsenic is reasonably well correlated with reduced plant growth by, and plant uptake of arsenic. For most plants, levels of arsenic in the edible portion of the plant are well below the critical concentration for animal or human consumption, even when severe phytotoxicity occurs. Alleviation of arsenic phytotoxicity has been attempted by increasing the soil pH, by use of iron or aluminum sulfate, by desorbing arsenate with phosphate and subsequent leaching, and by cultural practices such as deep plowing. Only limited benefits have accrued from these procedures the cost of which is often prohibitively high. Since attempts to reduce arsenic toxicity have not been very successful, its excessive accumulation in soils should be avoided.
SI quantities and units for American Medicine
Authors: E. R. Powsner
Journal: JAMA (1984)

The International System of Units (SI) is an extension of the metric system and was approved by the General Conference on Weights and Measures in 1960. The SI, expanded for the health professions, has been adopted by most European countries and is being adopted by Canada. Proponents of the SI for American medicine see intrinsic merit in its coherent units and believe international communication among physicians requires its adoption. Opponents fear that conversion to the SI is little more than "tinkering" and that any changes from the present system are potentially dangerous. Adoption of some of the less controversial portions of the SI has been recommended by the AMA Council on Scientific Affairs; consideration by the House of Delegates is anticipated. (<i>JAMA</i>1984;252:1737-1741)
Successful Strategies in Adult Immunization
Journal: JAMA (1991)

Safe and effective vaccines are available to prevent pneumococcal disease and influenza and hepatitis B virus (HBV) infections among persons in the United States; however, a substantial number of adults at increased risk for these preventable infections remain unvaccinated. This report describes collaborative public and private efforts to increase the vaccination of adults and highlights National Adult Immunization Awareness Week, October 27-November 2, 1991.
THU0048 1,25(OH)2D3 Inhibits Th17 Polarization and RORC Expression Through GATA3-Dependent and -Independent Mechanisms
Authors: W. Dankers, J. P. van Hamburg, A.-M. M. Mus, P. S. Asmawidjaja, J. P. van Leeuwen, R. W. Hendriks, L. Boon, E. M. Colin, E. Lubberts
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Vitamin D has suppressive effects on autoimmune diseases, such as rheumatoid arthritis (RA). Regulation of Th17 cell activity is an important mechanism by which vitamin D exerts these effects. Aside from inhibiting Th17 cytokines and the Th17 transcription factor Rorγt, vitamin D induces IL-4 and GATA3. Interestingly, GATA3 over-expression inhibits experimental Th17-mediated autoimmunity. <h3>Objectives</h3> We studied the contribution of GATA3 in vitamin D-mediated suppression of Th17 polarization. <h3>Methods</h3> CD4<sup>+</sup> T cells were sorted from patients with early RA, naïve DBA-1 mice, DBA-1 mice immunized with collagen type II (CII) or naïve CD2-GATA3 transgenic mice and cultured under T helper cell polarizing conditions with or without 1,25(OH)<sub>2</sub>D<sub>3</sub>, the active form of vitamin D. <h3>Results</h3> 1,25(OH)<sub>2</sub>D<sub>3</sub> inhibits Th17 polarization in CD4+ cells from both non-immunized and CII-immunized mice, while up-regulating IL-4 and GATA3 expression. In these cultures, IL-4 inhibition partly reversed the vitamin D-mediated inhibition of Th17 polarization. Moreover, GATA3 over-expression reduces Th17 differentiation to a lower level than 1,25(OH)<sub>2</sub>D<sub>3</sub>. Interestingly, combining GATA3 over-expression and 1,25(OH)<sub>2</sub>D<sub>3</sub> treatment reduced IL-17A and Rorγt expression even further. Furthermore, gene-expression analysis showed that NFAT-C2, which is involved in IL-17A production, was down-regulated by 1,25(OH)<sub>2</sub>D<sub>3</sub>. In addition, in T cells from patients with RA, 1,25(OH)<sub>2</sub>D<sub>3</sub> inhibited Th17 cytokine and RORγt expression and induced IL-4 and GATA3 expression. <h3>Conclusions</h3> These data show that vitamin D-mediated regulation of Th17 polarization occurs through GATA3-dependent mechanisms, including direct effects on RORγt expression and IL-4-mediated inhibition of Th17 polarization. Moreover, GATA3-independent mechanisms are involved that may include modulation of NFAT-C2 expression. <h3>Disclosure of Interest</h3> None Declared
Bureau of Investigation
Journal: JAMA (1928)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
"THE PIRQTJET SYSTEM OF NUTRITION AND ITS APPLICABILITY TO AMERICAN CONDITIONS"
Journal: JAMA (1921)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Enzyme Induction and Acute Endocrine Effects in Prepubertal Female Rats Receiving Environmental PCB/PCDF/PCDD Mixtures
Authors: Mei-Hui Li, Larry G. Hansen
Journal: Environmental Health Perspectives (1996)

Air, subsurface soil, and superficial dust from a National Priorities List landfill located in southern Illinois were sampled to determine their potential toxicities. The major components of these landfill extracts were polychlorinated biphenyls (PCBs), with significant amounts of polychlorinated dibenzofurans (PCDFs) and small amounts of polychlorinated dibenzodioxins (PCDDs). The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalency factor approach has been proposed to estimate the toxic potency of complex mixtures of chlorinated aromatics for environmental risk assessment. However, most components of environmental residues are nonplanar and do not act as aryl hydrocarbon (Ah) receptor agonists, so there is a great risk of not identifying adverse responses that are not dioxinlike. We used a 2-day prepubertal female rat bioassay to examine multiple biological responses, including both dioxinlike and nondioxinlike effects from these landfill extracts. As expected, both types of effects were detected. The soil and dust extracts produced similar dose-response relationships for 7-ethoxyresorufin O-deethylase, 7-pentoxyresorufin O-depentylase, 7-benzyloxyresorufin O-debenzylase, and 4-nitrophenol UDP-glucuronyltransferase induction; the dose response for the air extract deviated from the other two extracts. Soil, dust, and air extracts effectively reduced serum total thyroxine (T4) with similar dose-response relationships, despite the significantly different TCDD toxic equivalent (TEQ) values of these three extracts. Both soil (346 mg PCB/kg) and air (175 mg PCB/kg) extracts caused a greater than 30% increase in uterine wet weight. This study suggests that a more comprehensive approach is required to improve current risk assessment of environmental mixtures. TCDD TEQs reflect only a portion of effects and may especially underpredict effects on T4.
Intensive Care
Authors: Brenda Butka
Journal: Annals of Internal Medicine (2017)

Ad Libitum4 July 2017Intensive CareBrenda Butka, MDBrenda Butka, MDFrom Nashville, Tennessee.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/M17-0257 Audio Reading - “Intensive Care” Audio. Virginia L. Hood, MD, reads "Intensive Care," by B. Butka. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Mr. Jones stands outside the glass, looks inat his foreign wife. Alien,her only language numbers, her only songvariations on an oscilloscope. The audiencerecognizes wild new rhythms:she is pushing back frontiers, shemarches forward.In this cool light,eight floors above the parking lot,she transforms.A bag of bone and water,flesh becomes irrelevant, she becomeswhat she was not.A silent grotesque, she sings her song.The room sighs, is more alive than this centerpiece.Her chest rises and falls in time with machines.As the sun warms an aqua morning sky,a hidden crescendo.Mr. Jones tells Mrs. Jones good-bye. Author, Article, and Disclosure InformationAffiliations: From Nashville, Tennessee.Current Author Address: Brenda Butka, MD; e-mail, brenda.[email protected]edu. PreviousarticleNextarticle Advertisement Audio Reading - “Intensive Care” Audio. Virginia L. Hood, MD, reads "Intensive Care," by B. Butka. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ FiguresReferencesRelatedDetails Metrics 4 July 2017Volume 167, Issue 1Page: 65KeywordsBoneFallsThorax ePublished: 4 July 2017 Issue Published: 4 July 2017 Copyright & PermissionsCopyright © 2017 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Do health care premiums affect patient enrollment?
Authors: D. A. Barr
Journal: JAMA (1994)

<h3>To the Editor.</h3> —While I concur with many of the assertions of Rice et al<sup>1</sup>in their essay identifying weaknesses inherent in the Jackson Hole approach to health care, one of their most important arguments is, in my experience, false. They state, "[A]lthough consumers would be expected to respond somewhat to premium differences when choosing their health plans, the magnitude of this response does not appear to be particularly great," and go on to conclude that "relying on enrollees' cost consciousness is a fundamentally weak strategy." As part of a study I have done, I was provided data by the Department of Medical Economics of the Northern California Kaiser Permanente Health Care Plan (KP) for the period 1985 to 1989. This was a period in which KP experienced dramatic swings in membership, going from a net membership loss in the third quarter of 1985 to an increase of more
Human ingestion of a 'superwarfarin' rodenticide resulting in a prolonged anticoagulant effect
Authors: R. A. Lipton
Journal: JAMA (1984)

IN THE past decade, several novel hydroxycoumarin anticoagulants have been developed as rodenticides. These so-called superwarfarins have been developed to deal with the serious public health problem of warfarin resistance in rodents. This genetically determined resistance appears rapidly in wild rats and mice because it is transmitted as a dominant trait. <h3>Report of a Case</h3> A 31-year-old woman with a long history of mental illness and many hospitalizations for acute psychoses ingested over a two-day period approximately thirty 50-g packages of a commercial rodenticide (Talon-G) that contains brodifacoum. She purchased the rodenticide in a hardware store. On April 18,1983, five days after the ingestion, her family had her admitted to the hospital's psychiatric unit, and she was seen in medical consultation. Her BP was 120/70 mm Hg; pulse rate, 78 beats per minute; and respirations, 18/min. There were no orthostatic changes in the vital signs. There were multiple superficial slash
Influenza
Authors: John H. Dingle
Journal: New England Journal of Medicine (1947)

INFLUENZA is an old and well established disease that has appeared in epidemics and pandemics since before the seventeenth century. Despite its long history, however, the cause or causes of both epidemic and pandemic influenza remained unknown until fifteen years ago. Since then, remarkable progress in the methods of study and knowledge of epidemic influenza has taken place: epidemic influenza has been established as a virus infection caused by one or the other of two very similar agents; methods have been devised that have permitted chemical and physical characterization of the viruses; re-evaluation of the clinical and epidemiologic nature of . . .
Public Health Papers and Reports
Journal: JAMA (1892)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Unresolved issue: Do drinkers have less coronary heart disease?
Authors: E. R. Gonzalez
Journal: JAMA (1979)

The notion that a bit of imbibery is good for the heart is causing concern among physicians who fear that it will give people a new rationalization for drinking. At the recent American Heart Association (AHA) meeting in Anaheim, Calif, William P. Castelli, MD, medical director of the Framingham Heart Study, reiterated material from his recent editorial in<i>JAMA</i>(242:2000, 1979): "Can you tell people to take two drinks a day and stop? I can't see telling someone to start. That person could be prone to alcoholism, and you could end up destroying his family." Castelli echoed the concern of the AHA Nutrition Committee, which issued an advisory in October stating that "it would be premature to recommend a change in anyone's drinking habits.... Furthermore, there is no doubt that the dangers of acute or chronic excessive alcohol intake far outweigh any theoretical beneficial effect on HDL [high-density lipoprotein] cholesterol."
ANTITOXINS.
Authors: E. MARK HOUGHTON
Journal: JAMA (1898)

As we note the army of workers in the field of bacteriology, and the vast amount written on the subject, we can hardly realize that it has been scarcely twenty years since the germ was first demonstrated as the cause of a disease, and less than three years since Behring and Roux published perfected methods for preparing and administering diphtheria antitoxin. During this short period of discovery, recognized but unexplained truths have been elucidated, and many new facts have been brought to light. Many of the facts of immunity have been known since prehistoric ages, but prevailing theories regarding these questions were at fault: consequently, clinical interpretations and treatment have been fallacious. Before studying the antitoxins, it will be well to take a brief historical survey of the knowledge already acquired regarding the facts of immunity and the theories put forth to explain these facts. Pasteur in 1880 began his
Editorial: Adult respiratory-distress syndrome.
Journal: BMJ (1975)

PAS23 and possibly also isoniazid24 can interfere with the estimation of SGOT to produce spuriously high values.Some means of predicting a patient's hypersensitivity to antituberculous drugs is urgently needed.Unfortunately, in vitro tests such as the lymphocyte transformation test, in which the patient's lymphocytes are exposed to the drug under suspicion, have yielded conflicting results for antituberculous agents.25-28
Emerging concepts in the treatment of HIV infection in children
Authors: P. A. Pizzo
Journal: JAMA (1989)

<h3>SELECTED CASE</h3> A 16-MONTH-OLD child was considered to have human immunodeficiency virus (HIV) infection at 4 months of age when she developed acute interstitial pneumonia that was documented by open lung biopsy to be caused by<i>Pneumocystis carinii</i>. The patient's mother was known to be HIV seropositive, having presumably acquired the infection from sexual intercourse with the child's father, a known intravenous drug user. The patient's episode with<i>P carinii</i>was stormy, requiring first sulfamethoxazole and trimethoprim and, later, pentamidine isethionate and ventilatory support to reverse the pneumonic process. Following this episode of pneumonia, the child's subsequent course was characterized by recurrent episodes of otitis media and thrush, failure to thrive, hepatosplenomegaly, and a slowed acquisition of normal developmental milestones. At the time of her referral to the Pediatric Branch, National Cancer Institute, for antiretroviral therapy, her CD4 lymphocyte count was 0.115 × 10<sup>3</sup>/L and the CD4/CD8 lymphocyte
Intravascular ultrasound-guided provisional stenting in small coronary stenosis Alexandre Abizaid, Augusto D. Pichard, Roxana Mehran, Gary S. Mintz, Luis Gruberg, Javed Ahmed, Gregg W. Stone, Andrea S. Abizaid, Lowell F. Salter, Kenneth M. Kent, George Dangas, Martin B. Leon. Cardiovascular Research Foundation, New York, NY, Washington Hospital Center, Washington, DC
Journal: Journal of the American College of Cardiology (2001)