tbuckley/cabot-search huggingface dataset:
7Crispr/Cas9 gene editing reveals novel tertiary constraints in clustered miRNA processing
Authors: L Lataniotis, A Albrecht, F O Kok, CAL Monfries, L Benedetti, N Lawson, S Hughes, K Steinhofel, M Mayr, A Zampetaki
Journal: Cardiovascular Research (2018)

Introduction: MicroRNAs (miRNAs) play an important role in the cellular function.They often form families, with members sharing high sequence homology, a property that hampers miRNA research as there is a lack of elegant tools for specific miRNA manipulation.Purpose: To establish a reliable workflow for miRNA inhibition using genome editing.Methods and Results: This study focused on miR-195, a member of the miR-15 family and employed the CRISPR-Cas9 system.To this end we generated mouse vascular smooth muscle cells (VSMCs) stably expressing Cas9 nuclease.Cells were then transfected with an in vitro transcribed single guide RNA targeting the mir-195 gene.T7 endonuclease I assay (T7EI) and Sanger sequencing confirmed efficient editing.QPCR demonstrated effective decrease of miR-195 but not of miR-15a and miR-16, two highly expressed members of the miR-15 family in VSMCs.Surprisingly, we noticed that the expression of miR-497 was also decreased in edited cells.Noteworthy, miR-195 and miR-497 form a miRNA cluster and are co-transcribed as a primary transcript.No gene editing of the mir-497 genomic locus was detected by T7EI and sequencing.Computational simulation predicted that mutations of the miR-195 stem loop led to changes in the three dimensional structure of the primary miR-497195 transcript that could affect its processing to mature miRNAs.Similar findings were obtained for the miR-143145 cluster.MiR-143 and miR-145a, two miRNAs that do not belong to the same family, show no sequence homology and play a pivotal role in vascular biology.Specific targeting of the mir-145a locus effectively inhibited the expression of both miRNAs of the cluster while no genomic editing was observed for the mir-143 locus.On the contrary, gene editing in the miR-1792 and miR-106b25 clusters, two miRNA clusters with a key function in the cardiovascular system, resulted in targeted miRNA inhibition.MiR-18a and miR-25, respectively, were targeted on each cluster.Specific editing only for the intended miRNA locus was observed and qPCR quantification indicated inhibition of the edited miRNA.No effect on the expression of other miRNAs occurred, for either of the two miR-1792 and miR-106b25 clusters.Detailed analysis of the gene editing in the four clusters revealed that the unintended inhibition of expression of clustered miRNAs coincides with disruption of sequence motifs of the terminal loop of the targeted hairpin.These results suggest that these elements are critical for the maturation not only of individual hairpins but the entire primary transcript in miRNA clusters.Conclusions: CRISPR/Cas9 emerged as a powerful tool that can offer novel insights into the maturation process of miRNAs in cardiovascular diseases.
OPTIC NERVE DISTURBANCES IN DISEASES OF THE POSTERIOR NASAL SINUSES
Journal: JAMA (1920)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The papillary muscle syndrome
Authors: G. E. Burch
Journal: JAMA (1968)

Normal mitral valve function is dependent upon several structures other than the valve leaflets alone. These structures are in anatomic continuity from the atrial muscle to the ventricular muscle and, in addition to the leaflets, include the valve ring, the chordae tendineae, conduction tissue, and the papillary muscles. For perfectly normal valve function, each of these structures must be (1) anatomically and functionally intact, (2) in appropriate spatial interrelationship, and (3) operative in correct temporal sequence with respect to related structures. Obviously, normal mitral valve function is dependent upon a rather complex and finely coordinated system. In the past, of all the structures in this system, the papillary muscles have received the least interest and study. Yet they are of paramount importance, and disturbances of their function are a common cause of mitral valve incompetence. <h3>Pathophysiology</h3> Causes of papillary muscle dysfunction may be outlined as follows: Circulatory insufficiency (ischemia) Angina
THE OUTLOOK IN THE STUDY OF DIABETES
Journal: JAMA (1921)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
5?/5? isomerization during enterohepatic cycling: Bile acids of 5 dragons (AGAMIDAE) and . Department of Medicine, UCSD, San Diego, CA
Journal: Hepatology (1995)

Remains of the hominoidSivapithecus parvadaand a diversity of mammalian taxa are preserved at locality Y311 (ca. 10 Ma) in the Siwalik Nagri Formation of northern Pakistan. Bovids (Bovidae, Artiodactyla) are the most abundant mammals next to tragulids (Tragulidae, Artiodactyla) at locality Y311 and provide a means for reconstructing the paleoenvironments that would have been available toSivapithecus parvada. A functional model indicates a linkage between habitat and several femoral characters among extant bovids. Based on this model, we infer that forested habitats predominated at locality Y311 but that some less densely covered areas may also have been present. Paleoenvironments in the earlier Chinji Formation appear comparable to those at locality Y311, although the presence of a continuous canopy in the former is more certain. Thus, adaptive changes in the bovid fauna from the Chinji through the Nagri Formations appear to have preceded the shift to predominantly C4 grasslands which, based on other lines of evidence, occurred locally (and possibly globally) between 8 and 6 Ma. The paleoenvironments of locality Y311 and the Chinji Formation localities appear different from the paleoenvironment ofKenyapithecusat Fort Ternan in Kenya, where the presence of continuous canopy is unlikely. The Fort Ternan fauna is dominated by two genera of bovids. One of these is adapted to light cover while the other appears better adapted to heavy cover.SivapithecusandKenyapithecuslived in different ecological settings probably characterized by varying degrees of vegetative cover.
Allied health education and accreditation
Journal: JAMA (1981)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Efficacy and safety of combination therapy with elobixibat and colestyramine for non-alcoholic steatohepatitis model mice
Authors: Michihiro Iwaki, Takaomi Kessoku, Anna Ozaki, Takashi Kobayashi, Yasushi Honda, Yuji Ogawa, Kento Imajo, Masato Yoneda, Satoru Saito, Atsushi Nakajima
Journal: Journal of Hepatology (2020)
Low-birth-weight infants born to adolescent mothers. Effects of coresidency with grandmother on child development
Authors: S. K. Pope
Journal: JAMA (1993)

<h3>Objective.</h3> —To explore the impact of young maternal age, coresidency with infant's grandmother, and other familial and environmental factors on development of low-birth-weight (LBW) infants. <h3>Design.</h3> —Prospective cohort analyses. <h3>Setting.</h3> —Eight medical institutions in different geographical locations participating in the Infant Health and Development Program. <h3>Participants.</h3> —Control population of 272 LBW, preterm infants enrolled in the Infant Health and Development Program born to mothers aged 15 to 24 years. <h3>Main Outcome Measure.</h3> —Child cognitive, behavioral, and health outcomes at 36 months' gestation-corrected age. <h3>Results.</h3> —Maternal age was not significantly related to child development. Coresidence with infant's grandmother was associated with improved cognitive and health outcomes. Maternal ethnicity, maternal verbal ability, and other environmental factors were also associated with child outcomes. <h3>Conclusions.</h3> —Findings of this study support the need for programs that include the extended family of at-risk infants, providing education and literacy skills to the mothers and encouraging participation of all care givers of the child. (<i>JAMA</i>. 1993;269:1396-1400)
Union internationale contre le cancer; Conférence Préparatoire tenue le 22 mars 1934 au Ministére de la Santé publique à Paris
Journal: JAMA (1934)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Incorrect Byline and Figure Terminology
Journal: JAMA (2014)

Michelle L. O’Donoghue, MD, MPH; Eugene Braunwald, MD; Harvey D. White, MBChB, DSc; Dylan L. Steen, MD; Mary Ann Lukas, MD; Elizabeth Tarka, MD; P. Gabriel Steg, MD; Judith S. Hochman, MD; Christoph Bode, MD; Aldo P. Maggioni, MD; KyungAh Im, PhD; Jennifer B. Shannon, MS; Richard Y. Davies, MS; Sabina A. Murphy, MPH; Sharon E. Crugnale, MS; Stephen D. Wiviott, MD; Marc P. Bonaca, MD, MPH; David F. Watson, MSc; W. Douglas Weaver, MD; Patrick W. Serruys, MD, PhD; Christopher P. Cannon, MD; for the SOLID-TIMI 52 Investigators
Divided Authority in Yellow Fever.
Authors: None W.
Journal: JAMA (1899)

New York, June 29, 1899. <h3>To the Editor:</h3> —No little popular interest has been aroused in New York by the announcement promulgated by Quarantine Officer Doty of the discovery of a serum which is believed to be prophylactic against yellow fever and may be considered to have curative value. This has become known through the announcement which Dr. Doty gave to the daily press, for the medical pronouncement has yet to be made in some one of the professional papers. The gist of the discovery set forth by Dr. Doty appears to be that his bacterial assistant, Dr. C. B. Fitzpatrick, has succeeded in isolating a germ or micro-organism from certain cases of yellow fever which were detained at the quarantine station on Swinburne Island in 1897, and that from the cultures of this germ he has produced an efficient serum. In this announcement Dr. Doty rather detracts from the
RIO DE JANEIRO
Journal: JAMA (1931)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Melanin Pigmentation
Authors: Thomas B. Fitzpatrick, Makoto Seiji, A. David McGugan
Journal: New England Journal of Medicine (1961)

IN the basal layer of the human epidermis there are approximately two billion specialized unicellular secretory glands weighing as a group about 1 gm.1 , 2 These unique cells are called melanocytes because they secrete into the epidermal cells an amorphous brown pigment known as melanin (Greek melas, melanos, black). The biochrome, melanin, is largely responsible for the tinctorial differences that exist in the skin, hair and eyes of man. Its importance for man does not stop there, however. Melanin is necessary, if not essential, for survival; without melanin, man would be required to live under cover or reside in the sunless . . .
NIH Prepares to Launch Precision Medicine Study
Journal: Cancer Discovery (2016)

The NIH has awarded $55 million to several institutions to launch the Precision Medicine Initiative Cohort Program, which aims to enroll at least 1 million Americans between 2016 and 2020 in a long-term study. Organizers say the program will facilitate collaboration among researchers, potentially accelerating the development of personalized therapies for cancer and other diseases.
Implantation of a cardioverter/defibrillator without thoracotomy using a triple electrode system
Authors: S. Saksena
Journal: JAMA (1988)

This article describes the implantation of an automatic implantable cardioverter/ defibrillator in a 69-year-old man with coronary artery disease and recurrent sustained ventricular tachycardia without the need for a thoracotomy/sternotomy and epicardial electrodes. The patient underwent serial electrophysiological evaluation that revealed drug-refractory ventricular tachycardia. Surgical ablation was considered and rejected due to advanced, severe, pulmonary disease. A triple electrode system using two transvenous catheter electrodes and a submuscular patch electrode in the left midaxillary line was employed. A single shock was delivered simultaneously over two spatially distinct current pathways and reproducibly defibrillated ventricular fibrillation with energies less than or equal to 10 J. The automatic implantable cardioverter/defibrillator was implanted in the anterior abdominal wall and demonstrated reproducible termination of ventricular tachycardia and ventricular fibrillation. (<i>JAMA</i>1988;259:69-72)
Abstract 4199: HPV prevalence of cervical cancer in the United States 2014-2015
Authors: Jacqueline M. Mix, Elizabeth Unger, Troy Querec, Trevor Thompson, April Greek, Thomas Tucker, Charles Lynch, Edward Peters, Mona Saraiya
Journal: Cancer Research (2019)

Abstract INTRODUCTION: To determine the prevalence of human papillomavirus (HPV) types in invasive and in situ cervical cancers identified in three US population-based cancer registries (PBCRs) approximately 10 years after HPV vaccine implementation. METHODS: Archived tissue from invasive cervical cancer (ICC) and cervical carcinoma in situ (CCIS) diagnosed between 2014 and 2015 were identified from three PBCRs in Iowa, Kentucky, and Louisiana. HPV genotyping was performed using Linear Array (Roche Diagnostics); follow-up testing of HPV-negative samples was done with INNO-LiPA HPV Genotyping Assay (Innogenetics). Type-specific weighted HPV prevalences among ICC and CCIS were estimated using the hierarchical proportion to HPV 16 and 18 oncogenic genotypes included in all vaccine formulations (16/18) and protection from five additional types in the 9-valent vaccine (31/33/45/52/58). Comparisons were made with a similar study of seven PBCRs with cervical tumor tissue obtained from diagnoses between 1994 and 2005. RESULTS: A total of 363 ICC and 350 CCIS were successfully genotyped. Prevalence of HPV16/18 and HPV31/33/45/52/58 types were: ICC: [16/18: 69%, 31/33/45/52/58: 10%] and CCIS: [16/18: 68%, 31/33/45/52/58: 22%]. HPV prevalence in the 1994-2005 study was: ICC (n=777): [16/18: 67%, 31/33/45/52/58: 14%] and CCIS (n=481): [16/18: 61%, 31/33/45/52/58: 22%]. No overall changes were observed in the prevalence of HPV16/18 and HPV31/33/45/52/58 between the two studies. CONCLUSIONS: Given the long latency period of HPV-related cancers, continuing to monitor HPV prevalence in invasive and in situ cervical cancers will be important for evaluating the impact of the HPV vaccine on genotype prevalence. Citation Format: Jacqueline M. Mix, Elizabeth Unger, Troy Querec, Trevor Thompson, April Greek, Thomas Tucker, Charles Lynch, Edward Peters, Mona Saraiya. HPV prevalence of cervical cancer in the United States 2014-2015 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 4199.
La réduction gastrique endoscopique avec un dispositif de suture “endoluminal”: un essai prospectif multicentrique avec un suivi à 1 an
Journal: Endoscopy (2018)

La prise en charge de l’obésité est devenue un enjeu de santé publique. Les techniques de réduction volumique de la cavité gastrique sont réalisées depuis plusieurs années de manière chirurgicale, avec un taux de complication élevé. La réalisation de gastroplastie par voie endoscopique, technique moins invasive, se développe dans cette indication depuis peu.
Fibrinolytic Agents
Authors: Kenneth M. Moser
Journal: JAMA (1961)

<h3>To the Editor:—</h3> Differences of opinion, theory, and method are to be expected in any complex new field of medical investigation. While these differences are well recognized by the few physicians working on a specific problem, they are not apparent to physicians engaged primarily in other pursuits. Therefore, when the data and conclusions of one group in a highly specialized field are published, such information may be generally interpreted as fact rather than as representative of one point of view. These considerations have prompted this belated comment on an article by Fletcher and asso associates which appeared on Feb. 27, 1960 inThe Journal; this report seems seems to have promoted confusion among physicians regarding the status of fibrinolytic agents. The methods used by Fletcher and associates and their interpretation of data led them to express the opinion that one fibrinolytic agent of the activatorplasmin type (Actase) might not prove effective as a clot dissolvant in man. This
Methemoglobinemia resulting from absorption of nitrates
Authors: J. C. Harris
Journal: JAMA (1979)

Three cases of toxic methemoglobinemia resulted from absorption of nitrate salts through burned skin areas. In addition to the unusual route of intoxication, this report emphasizes the occupational hazard of methemoglobinemia in workers exposed to nitrogen salts and the importance of rapid diagnosis and treatment. The diagnosis of methemoglobinemia should be suspected in any case of cyanosis refractory to oxygen therapy. Appropriate treatment should include oxygen, methylene blue, and exchange transfusion in clinically severe cases. (<i>JAMA</i>242:2869-2871, 1979)