BIOLOGY
Authors: J. H. Kim, H. B. Song, D. H. Kim, K. D. Park, J. H. Kim, J. H. Kim, B. J. Lee, D. H. Kim, J. H. Kim, S. Khatua, E. Kalkan, R. Brown, M. Pearlman, T. Vats, L. Abela, G. Fiaschetti, T. Shalaby, E. Grunder, M. Ma, J. Grahlert, M. Baumgartner, U. Siler, N. Nonoguchi, H. Ohgaki, M. Grotzer, J.-i. Adachi, T. Suzuki, K. Fukuoka, T. Yanagisawa, K. Mishima, T. Koga, M. Matsutani, R. Nishikawa, I. Sardi, L. Giunti, C. Bresci, S. Cardellicchio, M. Da Ros, A. M. Buccoliero, S. Farina, M. Arico, L. Genitori, M. Massimino, L. Filippi, A. Erdreich-Epstein, H. Zhou, X. Ren, M. Schur, T. B. Davidson, L. Ji, R. Sposto, S. Asgharzadeh, Y. Tong, E. White, M. Murugesan, B. Nimmervoll, M. Wang, D. Marino, D. Ellison, D. Finkelstein, S. Pounds, D. Malkin, R. Gilbertson, C. Eden, B. Ju, M. Murugesan, T. Phoenix, H. Poppleton, C. Lessman, M. Taylor, R. Gilbertson, I. Sardi, G. la Marca, S. Cardellicchio, M. Da Ros, S. Malvagia, L. Giunti, V. Fratoni, S. Farina, M. Arico, L. Genitori, M. Massimino, M. G. Giovannini, F. Giangaspero, M. Badiali, V. Gleize, S. Paris, L. Moi, S. Elhouadani, A. Arcella, R. Morace, M. Antonelli, F. Buttarelli, K. Mokhtari, M. Sanson, S. Smith, J. Ward, M. Wilson, C. Rahman, F. Rose, A. Peet, D. Macarthur, R. Grundy, R. Rahman, S. Venkatraman, D. Birks, I. Balakrishnan, I. Alimova, P. Harris, P. Patel, N. Foreman, R. Vibhakar, H. Wu, Q. Zhou, D. Wang, G. Wang, D. Dang, E. Pencreach, A. Nguyen, E. Guerin, C. Lasthaus, D. Guenot, N. Entz-Werle, R. Unland, S. Schlosser, N. Farwick, T. Plagemann, G. Richter, H. Juergens, M. Fruehwald, C.-L. Chien, Y.-H. Lee, C.-I. Lin, J.-Y. Hsieh, S.-C. Lin, T.-T. Wong, D. M.-T. Ho, H.-W. Wang, S. Lagah, I.-L. Tan, S. Malcolm, R. Grundy, R. Rahman, Y. Majani, S. Smith, R. Grundy, R. Rahman, D. G. van Vuurden, E. Aronica, L. E. Wedekind, E. Hulleman, D. Biesmans, M. Bugiani, W. P. Vandertop, G. J. L. Kaspers, T. Wurdinger, D. P. Noske, P. M. Van der Stoop, D. G. van Vuurden, S. Shukla, L. E. Wedekind, G. K. Kuipers, E. Hulleman, D. P. Noske, T. Wurdinger, W. P. Vandertop, B. J. Slotman, G. J. L. Kaspers, J. Cloos, T. Sun, N. Warrington, J. Luo, S. Ganzhorn, U. Tabori, T. Druley, D. Gutmann, J. Rubin, P. Castelo-Branco, S. Choufani, S. Mack, D. Galagher, C. Zhang, T. Lipman, N. Zhukova, D. Martin, D. Merino, J. Wasserman, C. Samuel, N. Alon, J. Hitzler, J. C. Y. Wang, D. Malkin, G. Keller, P. B. Dirks, S. Pfister, M. D. Taylor, R. Weksberg, U. Tabori, P. Leblond, S. Meignan, A. Dewitte, F. Le Tinier, N. Wattez, E. Lartigau, A. Lansiaux, R. Hanson, I. Gordon, S. Zhao, K. Camphausen, K. Warren, N. M. Warrington, T. Sun, D. H. Gutmann, J. B. Rubin, A. Nguyen, C. Lasthaus, M. Jaillet, E. Pencreach, E. Guerin, D. Guenot, N. Entz-Werle, Z. Kovacs, E. Martin-Fiori, T. Shalaby, M. Grotzer, M. Bernasconi, B. Werner, C. Dyberg, N. Baryawno, J. Milosevic, M. Wickstrom, P. A. Northcott, M. D. Taylor, M. Kool, P. Kogner, J. I. Johnsen, M. Wilson, G. Reynolds, N. Davies, T. Arvanitis, A. Peet, A. Zoghbi, M. Meisterernst, M. C. Fruehwald, K. Kerl, B. Orr, M. Haffner, W. Nelson, S. Yegnasubramanian, C. Eberhart, A. Fotovati, S. Abu-Ali, P.-S. Wang, L. Deleyrolle, C. Lee, J. Triscott, J. Chen, S. Franciosi, Y. Nakamura, Y. Sugita, T. Uchiumi, M. Kuwano, B. Leavitt, S. Singh, A. Jury, C. Jones, H. Wakimoto, B. Reynolds, C. Pallen, S. Dunn, S. Fletcher, J. Levine, M. Li, N. Kagawa, R. Hirayama, Y. Chiba, N. Kijima, H. Arita, M. Kinoshita, N. Hashimoto, S. Izumoto, M. Maruno, T. Yoshimine
Journal: Neuro-Oncology (2012)

PURPOSE: To investigate whether tissue factor (TF) regulates fibroblast growth factor (FGF)-2-induced angiogenesis in retinoblastoma.METHODS: In an orthotopic transplantation mouse model of retinoblastoma, immunofluorescence staining for TF and CD31 (an endothelial cell maker) was performed.With treatment of FGF-2 (10 ng/ml), TF expression in human umbilical vein endothelial cells (HUVECs) was measured by Western blotting.To confirm the role of TF in tumor angiogenesis in retinoblastoma, anti-angiogenic activity of TF pathway inhibitor (TFPI) was investigated by treating TFPI on FGF-2-induced proliferation, migration and in vitro tube formation of HUVECs.In addition, inhibition of ERK1/2 phosphorylation by TFPI was measured by Western blot analysis.RESULTS: TF was highly expressed on vascular endothelial cells of retinoblastoma, co-localized with CD31.With FGF-2-induced proliferation of HUVECs, TF expression was significantly up-regulated.Interestingly, TFPI effectively inhibited FGF-2-induced proliferation, migration and in vitro tube formation of HUVECs, which was accompanied by inhibition of ERK1/2 phosphorylation.CONCLUSIONS: TF is involved in tumor angiogenesis of retinoblastoma via extracellular signal-regulated kinase pathway.