tbuckley/cabot-search huggingface dataset:
As Age, Income Go Up, Added Sugar Calories Go Down
Journal: JAMA (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Is Increasing the Copy Number of the Microrna Precursor in a Vector Really Helpful for Further Improving the Level of Mature Microrna in Stable Cells?
Authors: Hui Xu, Liang-Hu Qu
Journal: Hepatology (2010)

We are very pleased to discuss the related questions on the lentiviral vectors we used in our article.1 We also thank Wang et al. for the positive evaluation on our construction method. First, the copies of microRNA-122 (miR-122) precursor in the genome of stable cells can be identified by polymerase chain reaction, using primers covering the whole region of all miR-122 precursors. However, we did not perform this step because it is redundant. During lentiviral packaging, the region between the viral long terminal repeats (LTRs) of the transfer vector is packaged within the viral capsid.2 After infection of target cells, the viral RNAs are reverse-transcribed into the viral complementary DNAs, followed by the formation of double-stranded DNAs (dsDNAs), which could enter the nuclear DNA and integrate into the host genomes. During this process, only the integral/unspliced RNAs containing both the 5′ LTRs and 3′ LTRs could successfully form dsDNAs that are capable of integrating into the host genomes in a stable fashion.2 In other words, the viral DNA fragments integrated into the host genome must include the whole region between the viral LTRs of the transfer vector. Therefore, we could obtain the stable cells expressing multiple copies of the miRNA precursor, as expected. Second, we used a gradual number (one, two, four, and eight) of copies of miR-122 in vectors to construct miR-122–overexpressed stable cells. This is an advantageous method to further improve the expression level of miR-122. However, it has a major drawback. During packaging, because the viral RNAs transcribed from transfer vector possess the hairpin structures of pre–miR-122, they could be recognized and cleaved by Drosha and dicer. Therefore, as the copies of pre–miR-122 increased, the packaging effectiveness significantly decreased. The viral titer obtained using lentiviral vector carrying eight copies of pre–miR-122 was far lower than the titer obtained when using lentiviral vector carrying a single copy of pre–miR-122. For this reason, in the previous study, we did not construct vectors carrying more copies of pre–miR-122. Third, to determine if the multiple copies of pre-miRNA will form the predicted hairpin structures, we would check the secondary structure of viral RNA (including green fluorescent protein/pre-miRNAs/woodchuck hepatitis virus posttranscriptional regulatory element) using RNAstructure software before the construction. When four or eight copies of miR-122 hairpin were chained to express in one primary transcript, every copy of miR-122 precursor sequence could fold into the same hairpin structure as expected (data not shown). Hui Xu Ph.D.*, Liang-Hu Qu Ph.D.*, * Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol ,Sun Yat-sen University ,Guangzhou, China.
Heart Surgeon
Authors: Brenda Butka
Journal: Annals of Internal Medicine (2012)

Ad Libitum4 December 2012Heart SurgeonBrenda Butka, MDBrenda Butka, MDFrom Nashville, TN 37218.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-157-11-201212040-00014 Audio Reading - “Heart Surgeon” Audio. Michael A. LaCombe, MD, Annals Associate Editor, reads "Piano Man," by C.S. Williams, "Heart Surgeon," by B. Butka, and "Ménière's Attack," by B.E. Baldwin. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail I never liked that man.“He's the best,” you say.I know that much. Resigned,I close my eyes, mark the stethoscope's cold zigzag,Right, left, left, left, inhale, exhale.The rest I do not know.After, woozily, I am here again,for you, for you, as you,afraid for what we hope,cautiously touch my hand.The scar tattooed down my breastbonesays he was hereand has left his thumbprinton my heart.I close my eyes again,afraid for what we owe.Brenda Butka, MDNashville, TN 37218 Author, Article, and Disclosure InformationAffiliations: From Nashville, TN 37218.Current Author Address: Brenda Butka, MD; e-mail, [email protected]. PreviousarticleNextarticle Advertisement Audio Reading - “Heart Surgeon” Audio. Michael A. LaCombe, MD, Annals Associate Editor, reads "Piano Man," by C.S. Williams, "Heart Surgeon," by B. Butka, and "Ménière's Attack," by B.E. Baldwin. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ FiguresReferencesRelatedDetails Metrics 4 December 2012Volume 157, Issue 11Page: 826KeywordsHeartScarsSurgeonsTouch ePublished: 4 December 2012 Issue Published: 4 December 2012 Copyright & PermissionsCopyright © 2012 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Factors involved in crystal formation in cystinuria. Reduction of cystine crystalluria with chlordiazepoxide and during nephrotic syndrome
Authors: B. L. Fariss
Journal: JAMA (1968)

Six patients with persistent cystine crystalluria were given chlordiazepoxide hydrochloride (Librium) in a daily dose of 5 to 40 mg by mouth. While taking this drug, the patients had a prompt reduction in cystine crystalluria. When administration of the medication was stopped, cystine crystals were again noted but disappeared when the patients again began taking chlordiazepoxide. No other medications were prescribed during this study, and no attempt was made to control food or fluid intake. Also, a patient with cystine crystalluria was observed to have a decrease of cystine crystals in his urine with the development of the nephrotic syndrome during treatment with penicillamine (D-penicillamine). On reduction of his proteinuria, there was a prompt return of cystine crystals without a significant change in total cystine excretion. Possible mechanisms to explain these observations are under investigation.
The Epidermis
Authors: P. C. Anderson
Journal: JAMA (1965)

This book is affectionately dedicated to a master of investigative dermatology, Dr. Stephen Rothman, who was chairman of the Department of Dermatology at the University of Chicago until shortly before his death on the last day of August in 1963. Dr. Rothman's accomplishments as a teacher may outshine even his success as an investigator. Few persons in investigative dermatology did not somehow come under his stimulating influence. Most of this book concerns the stratum corneum and keratinization, the principal interests of contemporary investigators. The authors are unusually authoritative. The structure of alpha-keratin is described by Gunnar Swanbeck, the effects of vitamin A on keratinization by Honor Fell; Isser Brodey describes electronmicroscopic findings in the horny layer; Peter Flesch reviews the clinical anomalies in the stratum corneum, and Stephen Rothman, in an unusually interesting style, supplies historical perspective. The great importance of lipid synthesis in the development of a normal stratum
Antibody–Drug Conjugate Hits Elusive HER2+ Breast Tumors
Journal: Cancer Discovery (2013)

Abstract A biomarker analysis of the phase III EMILIA study in women with metastatic HER2-positive breast cancer suggests that the antibody–drug conjugate T-DM1 (Kadcyla; Genentech) works as well in patients who have PIK3CA mutations as in those who don't.
Highlights
Journal: JAMA (2014)

Maternal smoking during pregnancy has an adverse effect on infant lung development.Evidence from a primate model suggests vitamin C may mitigate some of the effect.To assess whether maternal vitamin C supplementation would improve pulmonary function of infants whose mothers smoked during pregnancy, McEvoy and colleagues randomly assigned 159 pregnant smokers to receive daily vitamin C supplements or placebo.The authors report that supplemental vitamin C improved newborn pulmonary function and decreased the incidence of wheezing in the first year of life.In an Editorial, Hall discusses maternal vitamin C supplementation and infant respiratory health.
SENSIBLE SANITARY MEASURES ON THE ISTHMUS.
Journal: JAMA (1906)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 1698: ERG protein expression and genomic rearrangement status in primary and metastatic prostate cancer - A comparative study of two monoclonal antibodies
Authors: Shyh-Han Tan, Martin Braun, Diane Goltz, Zaki Shaikibrahim, Wenzel Vogel, Diana Boehm, Veit Scheble, Albert Dobi, Falko Fend, Nicolas Wernert, Glen Kristiansen, Sven Perner
Journal: Cancer Research (2012)

Abstract Introduction and Objective: Overexpression of the ERG protein is highly prevalent in prostate cancer (PCa) and most commonly results from gene fusions involving the ERG gene. Recently, an N-terminal epitope targeted mouse and a C-terminal epitope targeted rabbit monoclonal anti-ERG antibody have been introduced for the detection of the ERG protein. Independent studies reported that immunohistochemical (IHC) stains with both monoclonal anti-ERG antibodies (ERG-MAbs) highly correlate with the underlying ERG gene rearrangement status. However, a comparative study of both antibodies has not been provided so far. Here, we are the first to compare the mouse ERG-MAb to the rabbit ERG-MAb for their concordance on the same PCa cohort. Furthermore, we assessed if the ERG protein expression is conserved in lymph node and distant PCa metastases, of which a subset underwent decalcification. Methods: We evaluated tissue microarrays of 278 specimens containing 265 localized PCa, 29 lymph node, 30 distant metastases, and 13 normal prostatic tissues. We correlated the ERG protein expression with the ERG rearrangement status using an ERG break-apart fluorescence in-situ hybridization (FISH) assay and IHC of both ERG antibodies. Results: ERG protein expression and ERG rearrangement status were highly concordant regardless of whether the mouse or rabbit ERG-MAb was used (97.8% versus 98.6%, respectively). Of interest, both ERG antibodies reliably detected the ERG expression in lymph node and distant PCa metastases, of which a subset underwent decalcification. If an ERG protein expression was present in localized PCa, we observed the same pattern in the corresponding lymph node metastases. Conclusions: This is the first study to comprehensively compare the two available ERG-MAbs. By demonstrating a broad applicability of IHC to study ERG protein expression using either antibody, this study adds an important step towards a facilitated routine clinical application. Further, we demonstrate that the clonal nature of the ERG rearrangement is not restricted to the genomic level, but proceeds in the proteome. Together, our results simplify future efforts to further elucidate the biological role of ERG in PCa. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1698. doi:1538-7445.AM2012-1698
Itching and the Spinothalamic Tracts
Journal: JAMA (1965)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Screening Mammography
Authors: L. S. Caplan, P. H. Gann
Journal: JAMA (1991)

<h3>To the Editor.—</h3> The recent article "Screening Mammography: A Missed Clinical Opportunity?"<sup>1</sup>identified the two most common reasons for women never having had a mammogram as (1) lack of awareness as to the need for one and (2) a failure of their physicians to recommend it. This lack of patient awareness can also be seen for knowledge of potential risk and protective factors for breast cancer. In a recent study that investigated the factors associated with patient delay in seeking medical help for self-discovered breast symptoms, we collected information to assess women's knowledge of possible risk factors and protective factors for breast cancer. Among 162 women presenting to a referral breast clinic in Worcester, Mass, for evaluation of self-discovered breast symptoms, 101 of them responded to questions in a self-administered questionnaire about whether certainfactors increase or decrease one's risk of developing breast cancer. As seen in the Table,
Coding Error Resulting in Change in Secondary Outcome Scores
Authors: Jean Kutner, Amy Abernethy
Journal: JAMA Internal Medicine (2018)

Jean S. Kutner, MD, MSPH; Patrick J. Blatchford, PhD; Donald H. Taylor Jr, PhD; Christine S. Ritchie, MD; Janet H. Bull, MD; Diane L. Fairclough, DrPH; Laura C. Hanson, MD; Thomas W. LeBlanc, MD; Greg P. Samsa, PhD; Steven Wolf, MS; Noreen M. Aziz, MD, PhD; David C. Currow, BMed; Betty Ferrell, PhD; Nina Wagner-Johnston, MD; S. Yousuf Zafar, MD; James F. Cleary, MD; Sandesh Dev, MD; Patricia S. Goode, MD; Arif H. Kamal, MD; Cordt Kassner, PhD; Elizabeth A. Kvale, MD; Janelle G. McCallum, RN, MSN; Adeboye B. Ogunseitan, MD; Steven Z. Pantilat, MD; Russell K. Portenoy, MD; Maryjo Prince-Paul, PhD; Jeff A. Sloan, PhD; Keith M. Swetz, MD; Charles F. Von Gunten, MD, PhD; Amy P. Abernethy, MD, PhD
Estimates of diagnostic accuracy should give confidence intervals
Journal: BMJ (1999)

Alcohol misusers reduce risks of surgery if they stop drinkingDuring recent years several studies have shown that alcohol misusers who drink at least five drinks a day have increased postoperative morbidity.On p 1311 Tønnesen et al report the results of the first randomised study of an intervention to reduce this morbidity-that is, one month of abstinence from alcohol (controlled with disulfiram) before surgery for colorectal resection.They found that the intervention group experienced a reduced rate of complications after the operation, possible because of improved organ function and reduced responses to surgical stress.The authors recommend preoperative assessment of alcohol misuse and withdrawal for one month before operation.
Correction for Results Section
Authors: David Lovas
Journal: JAMA Psychiatry (2016)

Evdokia Anagnostou, MD; Michael G. Aman, PhD; Benjamin L. Handen, PhD; Kevin B. Sanders, MD; Amy Shui, MA; Jill A. Hollway, PhD; Jessica Brian, PhD; L. Eugene Arnold, MD; Lucia Capano, MD; Jessica A. Hellings, MD; Eric Butter, PhD; Deepali Mankad, MD; Rameshwari Tumuluru, MD; Jessica Kettel, MD; Cassandra R. Newsom, PsyD; Stasia Hadjiyannakis, MD; Naomi Peleg, MSc; Dina Odrobina, BMSc; Sarah McAuliffe-Bellin, MEd; Pearl Zakroysky, MPH; Sarah Marler, MA; Alexis Wagner, BS; Taylor Wong, BS; Eric A. Macklin, PhD; Jeremy Veenstra-VanderWeele, MD
Variations in event rates between Canada and United States in the EPISTENT trial Eric A. Cohen, Michael Lincoff, Shelly Sapp, Jeffrey R. Burton, James E. Tcheng, Eric Topol, EPISTENT Investigators. Sunnybrook &amp; Women's College Health Sciences Centre, University of Toronto, Toronto, ON, Canada, Cleveland Clinic Foundation, Cleveland, OH
Journal: Journal of the American College of Cardiology (2001)
School Menus Improve but Need Work
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
AMA Schedules "Open House"
Journal: JAMA (1966)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
MASSAGE.
Authors: JOHN KERCHER
Journal: JAMA (1897)

Having had a number of years of personal experience in the application of massage; having seen massage applied by so-called professors of massage; also having read all available literature on the subject, I was amazed at the diversity of opinion and often utter lack of knowledge of the subject, as to what constitutes scientific massage. On the other hand there are a few books in the English language that treat the subject in an able and scientific manner. I was therefore prompted to write this article in order that the general practitioner may become better acquainted with the indications, contraindications, physiologic action, etc., of massage. I have for this reason taken up the subject in a scientific manner, hoping that the use of massage be thereby elevated and placed in the hands of physicians, where it belongs. <h3>DEFINITION OF MASSAGE.</h3> Dunglison, 1860—Massage; massement; massing-all have the same meaning<sup>1</sup>—shampooing. Webster's Unabridged
Columbus Cancer Conference: An Update on the Recent Advances in the Diagnosis and Treatment of Lung Cancer
Journal: JNCI Journal of the National Cancer Institute (1986)

Columbus Cancer Conference: An Update on the Recent Advances in the Diagnosis and Treatment of Lung Cancer Get access JNCI: Journal of the National Cancer Institute, Volume 77, Issue 5, November 1986, Page 1173, https://doi.org/10.1093/jnci/77.5.1173-b Published: 01 November 1986