SAT0292 Serum and Salivary Cytokines and Chemokines Levels as Disease Activity Biomarkers in Patients with Primary Sjögren's Syndrome
Authors: A.A. Tzec Pérez, R.E. Barbosa Cobos, G.E. Lugo Zamudio, L.T. Becerril Mendoza, M.A. Moreno Eutimio, N.G. Nieto Velázquez, C. Liceaga Escalera, M.E. Vélez Cruz, L.A. Montoya Pérez, A.A. Castillo Ortiz
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Evidence suggests that levels of different cytokines in primary Sjögren9s syndrome (pSS) are associated with cell infiltration degree within salivary and lacrimal glands and severity of the disease. Chemokines appear to contribute to the pathogenesis of pSS. <h3>Objectives</h3> To evaluate the association between serum and salivary Th1/Th2/Th17 cytokines and chemokines leves and disease activity in patients with pSS. <h3>Methods</h3> Case-control study, 18 patients with pSS and 13 healthy controls were included. Disease activity was assessed by EULAR Sjögren9s Syndrome Disease Activity Index (ESSDAI); serum and salivary IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, IL-17, IP10, MCP-1, MIG, RANTES and IL-8 levels were measured by bead-based assays following the sandwich immunoassay principle (BD Cytometric Bead Array; BD Biosciences). Differences between groups were analyzed using the Mann-Whitney U test, correlation was estimated by Spearman9s correlation coefficient. <h3>Results</h3> IL-17, IL-2, IL-4, IFNγ, MIG and RANTES salivary levels were statistically different between controls and patients with pSS (p&lt;0.005). Significant correlation (p&lt;0.005) was showed in patients between serum IL-6 and serum IL-12 (r=0.004), IL-10 (r=0.006); serum IL-12 and serum RANTES (r=0.008); serum IP-10 and serum MIG (r=0.000), RANTES (r=0.003); serum MIG and serum RANTES (r=0.014); salivary IL-17 and salivary IL-2 (r=0.004), IL-4 (r=0.001), INFγ (r=0.000), TNF (r=0.002), IL-10 (r=0.000); salivary IL-6 and salivary IL-2 (r=0.000), IL-4 (r=0.000), TNF (r=0.000), IL-10 (r=0.000), IL-8 (r=0.0003), MCP-1 (r=0.007); salivary IL2 and salivary IL4 (r=0.000), INFγ (r=0.006), TNF (r=0.001), IL10 (r=0.000); salivary IL4 and sallivary TNF (r=0.001), IL10 (r=0.000), MCP-1 (r=0.009); salivary TNF and salivary INFγ (r=0.002), IL-10 (r=0.000), IP-10 (r=0.006); salivary MCP-1 and salivary TNF (r=0.000), IL-10 (r=0.001), IL-8 (r=0.003). <h3>Conclusions</h3> Increased salivary Th1/Th2/Th17 cytokines and chemokines levels were found in pSS. No correlation was found between serum or salivary cytokines and chemokines levels and pSS disease activity. <h3>References</h3> Chen W, Cao H, Lin J, Olsen N, Zheng SG. Biomarkers for primary Sjögren syndrome. Genomics Proteomics Bioformatics 2015; 11:219–23. Bikker A, Moret FM, Kruize AA, Bijlsma JW, Lafeber FP, van Roon JA. IL-7 drives Th1 and Th17 cytokine production in patients with primary SS despite an increase in CD4 T cells lacking the IL-7Rα. Rheumatology Oxford 2012; 51: 996–1005. Lee YJ, Scofield RH, Hyon JY, Yun PY, Lee HJ, Lee EY, Lee EB, Song YW. Salivary chemokine levels in patients with primary Sjogren9s syndrome. Rheumatology Oxford. 2010; 49:1747–52. Katsifis GE, Rekka S, Moutsopoulos NM, Pillemer S, Wahl SM. Systemic and local interleukin-17 and linked cytokines associated with Sjögren9s syndrome immunopathogenesis. Am J Pathol 2009; 175:1167–1177. Delaleu N, Immervoll H, Cornelius J, Jonsson R. Biomarker profiles in serum and saliva of experimental Sjögren9s syndrome: associations with specific autoimmune manifestations. Arthritis Res Ther 2008; 10:1–14. <h3>Disclosure of Interest</h3> None declared