tbuckley/cabot-search huggingface dataset:
Abstract 17292: Ulk1 Plays a Crucial Role in Mitochondrial Autophagy in Cardiomyocytes
Authors: Toshiro Saito, Yoshiya Monden, Junichi Sadoshima
Journal: Circulation (2014)

Mitochondrial autophagy (MA) plays an important role in mediating mitochondrial (Mt) quality control. The molecular mechanism by which damaged mitochondria are removed during energy stress in cardiomyocytes (CMs) remains poorly understood. We investigated the roles of Atg7 and Ulk1, key regulators of autophagy, in mediating MA. Although cell viability, evaluated with CellTiter-Blue assays, was significantly lower (p&lt;0.05) in Ad-shRNA-Atg7 (sh-Atg7)-transduced CMs than in Ad-shRNA-Ulk1 (sh-Ulk1)- or Ad-shRNA-Control (sh-Cont)-transduced CMs at baseline, those after glucose deprivation (GD) were significantly greater (p&lt;0.05) in sh-Ulk1-transduced CMs than in sh-Atg7- or sh-Cont-transduced CMs. Although LC3-II and reduction of p62, markers of conventional autophagy, were indistinguishable in sh-Ulk1 and sh-Cont-transduced CMs, they were significantly suppressed in sh-Atg7-transduced CMs in comparison. GD significantly decreased (p&lt;0.05) the amount of mtDNA and citrate synthase activity, markers of Mt mass, in sh-Cont- or sh-Atg7-transduced CMs, but not in sh-Ulk1-transduced CMs. More CMs with depolarized mitochondria were observed in sh-Ulk1-transduced CMs than in sh-Cont- or sh-Atg7-transduced CMs after GD. Thus, Ulk1 plays a more important role in mediating CM survival and Mt clearance in response to GD than Atg7. Clearance of MA through lysosomes was evaluated with mitochondria-targeted (Mito)-Keima, with a dual excitation spectrum peaking at 440 and 560 nm, corresponding to neutral and acidic pH, respectively. Knockdown of Drp1 or Beclin1 abolished the fluorescent dots with a high 560nm/440nm ratio, which represent lysosomal translocation of Mito-Keima, suggesting that the high-ratio dots represent MA. In control CMs, high-ratio dots represented 3.6±0.5 % of the area after GD, indicating stimulation of MA. Although knockdown of Ulk1 significantly reduced the area to 0.7±0.4 % (p&lt;0.01, vs sh-Cont) after GD, that of Atg7 or p62 did not (4.9±1.3 % and 4.0±1.0 %). These results suggest that although Atg7 plays an important role in mediating autophagy at baseline, MA may take place even in the absence of Atg7 during energy stress. Furthermore, Ulk1 plays a major role in MA and CM survival in response to energy stress.
Periorbital heliotrope oedema as the only initial clinical manifestation of systemic lupus erythematosus in a primigravida
Authors: Y-S. Dai, H-C. Chiu
Journal: British Journal of Dermatology (2000)

Journal Article Periorbital heliotrope oedema as the only initial clinical manifestation of systemic lupus erythematosus in a primigravida Get access Y‐S. Dai, Y‐S. Dai Department of Dermatology, National Taiwan University Hospital, no. 7 Chung‐Shan S. Road, 100 Taipei, Taiwan Search for other works by this author on: Oxford Academic Google Scholar H‐C. Chiu H‐C. Chiu Department of Dermatology, National Taiwan University Hospital, no. 7 Chung‐Shan S. Road, 100 Taipei, Taiwan Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 143, Issue 3, 1 September 2000, Pages 679–680, https://doi.org/10.1111/j.1365-2133.2000.03753.x Published: 01 September 2000
Potential Mechanisms of Thyroid Disruption in Humans: Interaction of Organochlorine Compounds with Thyroid Receptor, Transthyretin, and Thyroid-Binding Globulin
Authors: Ann Oliver Cheek, Kelvin Kow, Jian Chen, John A. McLachlan
Journal: Environmental Health Perspectives (1999)

Organochlorine compounds, particularly polychlorinated biphenyls (PCBs), alter serum thyroid hormone levels in humans. Hydroxylated organochlorines have relatively high affinities for the serum transport protein transthyretin, but the ability of these compounds to interact with the human thyroid receptor is unknown. Using a baculovirus expression system in insect cells (Sf9 cells), we produced recombinant human thyroid receptor ss (hTRss). In competitive binding experiments, the recombinant receptor had the expected relative affinity for thyroid hormones and their analogs. In competitive inhibition experiments with PCBs, hydroxylated PCBs (OH-PCBs), DDT and its metabolites, and several organochlorine herbicides, only the OH-PCBs competed for binding. The affinity of hTRss for OH-PCBs was 10,000-fold lower (Ki = 20-50 microM) than its affinity for thyroid hormone (3,3',5-triiodothyronine, T3; Ki = 10 nM). Because their relative affinity for the receptor was low, we tested the ability of OH-PCBs to interact with the serum transport proteins--transthyretin and thyroid-binding globulin (TBG). With the exception of one compound, the OH-PCBs had the same affinity (Ki = 10-80 nM) for transthyretin as thyroid hormone (thyroxine; T4). Only two of the OH-PCBs bound TBG (Ki = 3-7 microM), but with a 100-fold lower affinity than T4. Hydroxylated PCBs have relatively low affinities for the human thyroid receptor in vitro, but they have a thyroid hormonelike affinity for the serum transport protein transthyretin. Based on these results, OH-PCBs in vivo are more likely to compete for binding to serum transport proteins than for binding to the thyroid receptor.
JAMA reference directories
Journal: JAMA (1978)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Ophthalmology.
Authors: None "Haller."
Journal: JAMA (1896)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
La talassemia (morbo di Cooley e forme affini)
Journal: JAMA (1952)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Teleradiology for a family practice center
Authors: E. A. Franken
Journal: JAMA (1989)

WE STUDIED the effect of teleradiology (TR) for radiological consultation in a real life situation with an independent family practice center. The hypotheses tested were (1) that TR provides acceptable diagnostic accuracy and (2) that timely radiological consultation is of value to the family practitioner. In prospective and controlled fashion we performed parallel interpretation of roentgenograms and TR images of these roentgenograms. In addition to evaluating the accuracy of the radiological consultation, we investigated the impact of this report on the family practice physician and patient. <h3>MATERIALS AND METHODS</h3> The family practice center of the University of Iowa, Iowa City, is an autonomous, separate unit of the University of Iowa Hospitals and Clinics. The study group consisted of 200 consecutive patients who underwent radiography at the family practice center. The study was limited to roentgenograms of the chest and extremities, consistent with radiation protection laws and practice styles in Iowa.
Crucial role of beta-arrestin-2 in linking fibrogenesis and cancer
Authors: Robert Schierwagen, Peter Dietrich, Judith Heinzen, Sabine Klein, Frank Uschner, Cristina Ortiz, Olaf Tyc, Sandra Torres, Sandra Rathmann, Richard Premont, Johanne Poisson, Pierre-Emmanuel Rautou, Glen Kristiansen, Jordi Gracia-Sancho, Marko Poglitsch, Isis Ludwig-Portugall, Thomas Walther, Zeinab Abdullah, Robert Lefkowitz, Mercedes Fernandez, Richard Moreau, Claus Hellerbrand, Krista Rombouts, Wolfgang Kastenmüller, Anna Mae Diehl, Jonel Trebicka
Journal: Journal of Hepatology (2020)
Effect of Asbestos on the Metabolism of Vasoactive Substances in Isolated Perfused Guinea Pig Lungs
Authors: Alain Cadieux, Serge Masse, Pierre Sirois
Journal: Environmental Health Perspectives (1983)

The measurement of pulmonary metabolism of three vasoactive substances and quantitative assessment of changes in lung morphology were performed in a long-term study of asbestos-exposed guinea pigs. Animals received intratracheal injections of a single dose of a sterile suspension of Canadian chrysotile B (5 mg), while control animals received only saline. Six months after the treatment, the guinea pigs were sacrificed, the lungs removed, perfused via the pulmonary artery and the metabolism of vasoactive substances was assessed (in vitro) in a cascade superfusion system. At the end of the experiments, the lungs were fixed in a glutaraldehyde solution for microscopic examination. The tissue response consisted of both inflammatory reaction of terminal and respiratory bronchioles and diffuse alveolar septal infiltration with interstitial fibrosis. The reaction was characterized at six months by a progressive bronchiolitis obliterans with fibroblastic proliferation and collagen formation. The development of the disease did not cause significant changes in the metabolism of acetylcholine and bradykinin. However, the metabolism of prostaglandin E2 decreased with the appearance of the bronchiolitis obliterans. Our results showed that asbestos exposure may produce early biochemical changes resulting in altered lung metabolism of vasoactive substances; these modifications could contribute to the pathogenesis of asbestosis.
Liver and intestinal propoxyphene metabolisms: An example of discreprancy in drug pre-systemic biotransformation in the rat Dept. of Gastroenterology, Experimental Surgery and Pharmacotherapy Laboratories, Catholic University of Louvain, Brussels
Journal: Hepatology (1995)

Previous studies have shown that several factors may influence the methylation of inorganic arsenic by rat liver in vitro (Buchet and Lauwerys, 1985). The present study attempts to assess the relevance of these observations in vivo. Like man, rat inactivates inorganic arsenic by methylation to monomethylarsoni (MMA) and dimethylarsinic (DMA) acids which are excreted in urine along with unchanged inorganic arsenic (Asi). The administration of S-adenosylmethionine alone or in association with reduced (GSH) or oxidized glutathione or acetylcysteine and the increase of hepatic GSH level by butylated hydroxytoluene pretreatment do not stimulate the urinary excretion of the methylated arsenic metabolites following a challenge dose of inorganic arsenic. Conversely a reduction of the hepatic GSH level by phorone pretreatment greatly modifies the metabolism of inorganic arsenic in vivo. A reduction exceeding 90% of the control value leads to a decreased urinary excretion of MMA and DMA and an increased urinary excretion of inorganic arsenic. This is also associated with an increased accumulation of inorganic arsenic in the liver. This suggests that a drastic reduction of GSH level in liver not only impairs the methylation of inorganic arsenic but also impairs its biliary excretion. When GSH depletion is less severe, the total amount of arsenic excreted in urine after a challenge dose of NaAsO2 is not significantly different from that found in unpretreated animals but the proportion of the three metabolic forms is different: MMA is reduced whereas Asi and DMA tend to increase. These changes resemble those found in patients with liver insufficiency (J. P. Buchet, A. Geubel, S. Pauwels, P. Mahieu, and R. Lauwerys (1984). The influence of liver disease on the methylation of arsenite in humans. Arch. Toxicol. 55, 151–154). Long-term pretreatment of rats with CCl4 slightly reduces the amount of MMA and DMA excreted in urine following a challenge dose of inorganic arsenic. This effect may result from a reduction of GSH transferase activity by CCl4. This study demonstrates the important role of liver GSH in the metabolism of inorganic arsenic in vivo
THYROID DISEASE, DIGITALIS, AND HYPOTHESES VALIDATED
Journal: JAMA (1966)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
In Memoriam.
Journal: JAMA (1907)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
WILHELM HIS, SR. (1831-1904)—EMBRYOLOGIST AND ANATOMIST
Journal: JAMA (1964)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Preventing neonatal herpes. The value of weekly viral cultures in pregnant women with recurrent genital herpes
Authors: N. J. Binkin
Journal: JAMA (1984)

To reduce the occurrence of neonatal herpes, laboratory screening with viral cultures during the last four to eight weeks of pregnancy has been recommended as a way to recognize women with subclinical herpes infection at delivery. Screening efforts have focused on a group of high-risk women, especially those with recurrent genital herpes infection. Using the technique of decision analysis, we examined the benefits, risks, and costs of viral culture screening for women with recurrent genital herpes. In a cohort of 3.6 million women, we estimate that screening would avert 11.3 neonatal deaths and 3.7 cases of severe retardation, but 3.3 women would die as a result of cesarean deliveries necessitated by culture results. Weekly viral cultures would diagnose one fourth of women with subclinical recurrent infection at delivery. The cost per case averted would be approximately $1.8 million. Future screening recommendations should consider not only the number of cases averted but also the effectiveness of screening and the costs in both maternal lives and medical care dollars.
MYELORRHAPHY.
Journal: JAMA (1902)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Functional significance of renal prostacyclin and thromboxane A2 production in patients with systemic lupus erythematosus
Authors: Carlo Patrono, Giovanni Ciabattoni, Giuseppe Remuzzi, Eliana Gotti, Stefano Bombardieri, Ombretta Munno, Giancarlo Tartarelli
Journal: Journal of Clinical Investigation (1985)

The following reference to the original description of the coupling technique of eicosanoids to L-[4,5-3H]leucine was inadvertently omitted in the Methods.
Terminal Complement Blockade With Pexelizumab During Coronary Artery Bypass Graft Surgery Requiring Cardiopulmonary Bypass: A Randomized Trial—Correction
Authors: Adam Wertz, Kenneth Wright, Joseph Ronda, Charles Czeisler
Journal: JAMA (2006)

SD, 16%; range, 35%-85%) (FIGURE).Between 21 and 61 minutes of wakefulness, the mean cognitive performance ranged from 83% to 86% of peak performance and was not significantly different from performance at subsequent points.The results for the female participant were not significantly different from the results for the male participants.Comment.Cognitive performance immediately on waking was worse than performance during subsequent sleep deprivation.We observed severe impairments within the first 3 minute of awakening, and others have reported severe performance impairments lasting up to 10 minutes following awakening, with effects on performance detectable for at least 2 hours. 1,2The degree of impairment is likely to be clinically important, given that changes in cognitive performance following 24 hours of sleep deprivation are reported to be comparable with those due to alcohol intoxication. 4,5 Our performance test required cognitive operations that use short-term memory, counting skills, speed of cognitive processing, and number fact and lexical retrieval.These skills are critical for many occupational tasks; however, the test did not simulate a specific operational task or indicate the effects of sleep inertia on performance in an operational setting.Further studies should measure this and should include more participants and broader populations.To extrapolate to medical personnel, such studies should measure the effects of interruption of naps and recovery sleep in on-call sleepdeprived individuals.However, given that sleep inertia increases with the depth of prior sleep, 1 the present results may have important implications for occupations in which sleep-deprived personnel are expected to perform immediately on awakening from deep sleep, including physicians, truck drivers and pilots arising from on-board sleeper berths, and public safety and military personnel.