tbuckley/cabot-search huggingface dataset:
Abstract 1070: Targeted Delivery of Macromolecules Using Ultrasound and Microbubbles is Regulated by Induction of Endocytosis and Pore Formation
Authors: Lynda J Juffermans, Bernadet D Meijering, Klazina Kooiman, Marcia Emmer, Annemieke van Wamel, Rene J Musters, Rob H Henning, Inge S Zuhorn, Leo E Deelman, Wiek van Gilst, Nico de Jong, Otto Kamp
Journal: Circulation (2008)

Ultrasound (US) contrast microbubbles are promising vehicles for local drug and gene delivery. However, the exact mechanisms behind intracellular delivery of therapeutic compounds remain to be resolved. We hypothesized that endocytosis and pore formation are involved during US and microbubble targeted delivery (UMTD) of therapeutic compounds. Primary bovine aortic endothelial cells were subjected to UMTD of fluorescent dextrans (4.4 – 500 kDa) using 1-MHz US during 30s with 0.22 MPa peak-negative pressure. Duty cycle: 6.2%, pulse repetition frequency 20 Hz. Confocal laser microscopy showed the localization of 155 and 500 kDa dextrans in distinct vesicles after UMTD, indicating uptake via endocytosis. Independently inhibiting the main routes of active cellular uptake, i.e. clathrin- and caveolae-mediated endocytosis and macropinocytosis, caused significant decreases of 50 – 70% in intracellular delivery of 4.4 – 500 kDa dextrans (p&lt;0.05). Furthermore, 3D immunofluorescence microscopy demonstrated dextran vesicles (500 kDa) to co-localize with both clathrin and caveolin-1. Finally, after energy depletion no uptake of 500 kDa dextran was observed after UMTD. In addition to triggering endocytosis, UMTD evoked transient pore formation, suggested by the homogeneous distribution through the cytosol of the 4.4 and 70 kDa dextrans. Formation of transient pores in the cell membrane was further supported by the influx of Ca2+ measured by live-cell fluorescence microscopy. This influx (peak mean fluorescence intensity (MIF) 186.4±3.4%) was not affected by the L-type Ca2+ channel blocker verapamil (MIF 172.5±3.2%), but significantly attenuated in a Ca2+ free buffer (MIF 111.1±2.0%, p&lt;0.001). Finally, cells pre-loaded with fluorescent dextrans showed a significant decrease (p&lt;0.05) of cytosolic fluorescence after US and microbubble exposure (4.4 kDa: 63.4±2.1%) and (155 kDa: 79.1±2.3%), compared to cells not exposed to US and microbubbles (100%). Endocytosis is a key mechanism of UMTD besides transient pore formation. The contribution of uptake via pores is dependent on molecular size. These findings are pivotal for optimizing US and microbubble intracellular delivery of therapeutic compounds.
ANCYLOSTOMIASIS AND BILHARZIASIS IN EGYPT
Journal: JAMA (1925)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Gaspare Aselli (1581-1626) The Lacteals
Journal: JAMA (1969)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 2110: WEE1 inhibition could reverse trastuzumab resistance by downregulation of PD-L1 in HER2-positive cancers
Authors: Meihua Jin, Ah-Rong Nam, Ji-Eun Park, Ju-Hee Bang, Kyoung-Seok Oh, Do-Youn Oh, Yung-Jue Bang
Journal: Cancer Research (2019)

Abstract Background: Trastuzumab in combination with chemotherapy is a standard of care for patients with HER2-positive breast and gastric cancer. Resistance mechanism to trastuzumab, anti-HER2 therapy, includes multiple pathways. Cancer cells could evade immune surveillance through high expression of programmed cell death ligand 1 (PD-L1) on cancer or immune cells. Among resistance mechanisms to trastuzumab, the role of PD-L1 modulation has not yet been discovered in HER2-positive cancers. WEE1 is involved in cell cycle progression and DNA damage response (DDR), and, interestingly, beyond DDR, it could modulate immunes. We aimed to evaluate the role of PD-L1 in trastuzumab resistance in HER2-positive cancers with/without immune cells and to explore WEE1 inhibitor as a trastuzumab resistance overcoming strategy by modulation of PD-L1. Methods: Four trastuzumab-resistant (HR) cells (SNU216HR, N87HR, SNU2670HR, SNU2773HR) were established from 2 HER2-amplified gastric cancer cells (SNU216, NCI-N87) and 2 HER2-amplified biliary tract cancer cells (SNU2670, SNU2773). For WEE1 inhibition, AZD1775 was used. MTT assay, colony formation assay, cell cycle analysis by FACS Calibur flow cytometer, and western blot were done. Results: All four HR cells showed PD-L1 upregulation compared with parental cells. When PD-L1 was knocked-down by transfection with si-PD-L1, anti-growth/proliferation effects were observed in MTT assay and colony forming assay. PD-L1 itself may promote cancer cell growth without interacting with immune cells. AZD1775 downregulated PD-L1 expression and induced cell cycle arrest at sub-G1 and G2/M phases in all 4 HR cells. The synergistic anti-proliferative effects were found in all 4 HR cells with co-treatment of trastuzumab and AZD1775. Conclusion: PD-L1 upregulation may contribute to trastuzumab resistance in HER2-positive cancer cells. Targeting WEE1-PD-L1 pathway might be a candidate strategy to overcome trastuzumab resistance in HER2-positive cancers. Citation Format: Meihua Jin, Ah-Rong Nam, Ji-Eun Park, Ju-Hee Bang, Kyoung-Seok Oh, Do-Youn Oh, Yung-Jue Bang. WEE1 inhibition could reverse trastuzumab resistance by downregulation of PD-L1 in HER2-positive cancers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2110.
HER2 status in breast cancer–an example of pharmacogenetic testing
Authors: Mark Kroese, Ron L Zimmern, Sarah E Pinder
Journal: Journal of the Royal Society of Medicine (2007)

The development of new drugs and associated pharmacogenetic tests will provide an increasing number of challenges to health care systems. In particular, how to evaluate their benefits, prioritize for commissioning purposes and implement a service to provide them in a timely manner. This paper presents an overview of HER2 testing for trastuzumab (Herceptin) treatment in breast cancer cases. Immunohistochemistry and fluorescence in situ hybridization laboratory techniques are described and their HER2 testing performances are compared. Future options for the national provision of HER2 testing by the National Health Service in the UK are also discussed.
Who will die in the ICU? APACHE II, ROC curve analysis, and, of course, Cleone
Journal: JAMA (1989)

<h3>To the Editor. —</h3> The article by Kruse et al<sup>1</sup>important contribution to efforts at predicting the survival of patients admitted to medical intensive care units. However, the authors' use of the area under the receiver operating characteristic (ROC) curve to evaluate the predictions is potentially misleading. It is hard to imagine that the ability of clinicians to estimate intermediate probabilities (ie, a 60% mortality) with precision influences clinical action. Only extremely high or extremely low estimates of the probability of dying provide information that might change clinicians' decisions in the intensive care unit.<sup>2</sup>Since a ROC curve is established by many intermediate predictions, the estimates of a mediocre predictor can still generate a relatively large area under the ROC curve and appear to have "good" predictive accuracy, but offer little practical clinical value. The relevance of any predictor of mortality is best evaluated by evaluating the correctness
Flowct: A Semi-Automated Workflow for Deconvolution of Immunophenotypic Data and Objective Reporting on Large Datasets
Authors: Cirino Botta, Catarina Maia, Cristina Pérez Ruiz, Irene Manrique, Juan José Garcés, Sara Rodríguez, Leire Burgos, Juana Merino, Aitziber Lopez Lopez, Noemi Puig, María Teresa Cedena, Artur Paiva, Marco Rossi, Pierosandro Tagliaferri, Piefrancesco Tassone, Massimo Gentile, Ivan M. Borrello, Laura Rosinol Dachs, Maria-Victoria Mateos, Juan-José Lahuerta, Joan Bladé, Jesús San-Miguel, Bruno Paiva
Journal: Blood (2019)
Advances in Surgery
Authors: M. Michael Eisenberg
Journal: JAMA (1972)

This is the fifth volume in a series of texts designed to review facets of surgery in which significant progress has been made within the past two years. It represents a high-quality "catch up" analysis of six areas of general concern to the practicing surgeon. The sections, written mostly by distinguished and experienced clinicians, include such diverse areas of interest as hemorrhoidectomy, advances in clinical diagnostic roentgenology, surgery of pancreatic, parathyroid, and adrenal endocrine tumors, diverticular disease of the colon, biologic principles affecting repair of flexor tendons, and intra-abdominal sepsis. A particularly timely section on the present status of tumor immunology is also included. Like its predecessors, this text is illustrated and each division ends with a detailed bibliography, numbering, in one instance, in excess of 300 references—enough, in short, for the more serious reader to pursue each covered area in as much depth as he might happen to want.
Verwertung der diagnostischen Laboratoriumsverfahren in der ärztlichen Praxis.
Journal: JAMA (1929)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Migraine
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
A Correction
Journal: BMJ (1918)

I~~~~~~~~~~~~~~~~~~&UZDICA Joumuz.463 inquiry should be extended.He thought the HIouse should have had the report of the committee before it.At the time this committee was appointed it was under- stood-although he knew that that had been denied-that under the terms of reference it would investigate arrange- nients in this country as well as in France.The medical service in the East ought also to have been included.Sir Henry Craik, as the representative of some 5,000 doctors, supported the view that some further form of compulsion was desirable.He had received comrmunications from some of his constituents complaining that certain men in advantageous positions were stealing away the practices of others.It was a very serious
Clinical Gastroenterology
Authors: Howard Schachter
Journal: JAMA (1970)

Designed for the practicing physician, this textbook provides a practical and informative clinical approach to disorders of the gastrointestinal tract. At the same time, it presents the reader with basic background information for understanding the pathophysiology of digestive diseases. Particular virtues of this text are the large number of generally clear illustrations (although some of the arrows are not clearly visible) and refreshing readability, featured by a direct and positive style of writing. The book is sturdily bound, and the quality of paper and legibility of print are excellent. The text is organized into nine units including diseases of the esophagus, stomach, peptic ulcer, small intestinal disorders, diseases of the colon and rectum, the gallbladder and biliary duct, pancreas, liver, and miscellaneous considerations, and comprising a total of 60 chapters. These sections receive varying emphasis but the entire field generally is well-covered. As would be expected with single authorship, opinions
Shock
Authors: John J. Byrne
Journal: New England Journal of Medicine (1966)

DURING the past year, numerous symposiums and monographs have been published to guide the practitioner and investigator in the diagnosis and treatment of shock.1 2 3 4 5 From this wealth of new information, 3 common varieties are clearly emerging: hypovolemia, vasodilatation; and an ineffective cardiopulmonary pump.Causes and Types Hypovolemic shock (cold shock) is produced by loss from the circulating vascular space to the exterior or internal body compartments, – that is, the peritoneum, pleural cavity or extracellular spaces. Usually, it is caused by injury, hemorrhage, burns, operative trauma and salt and water depletion, and it complicates peritoneal diseases such as peritonitis, pancreatitis . . .
HERNIA OF THE APPENDIX WITH FOREIGN BODY PERFORATION OF THE HERNIAL SAC
Journal: JAMA (1921)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Patogenesi e terapia della sindrome di Morgagni-Adams-Stokes
Journal: JAMA (1934)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Do Everything
Authors: Ronald A. Fronduti
Journal: Annals of Internal Medicine (1994)

Letters1 December 1994Do EverythingRonald A. FrondutiRonald A. FrondutiSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-121-11-199412010-00024 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Dr. Wynia's account of how he had convinced the attending physician of a dying patient with liver failure not to institute cardiopulmonary resuscitation (CPR) despite the instructions of the patient's wife to “do everything” expresses a laudable ethical stance but seems to place the physician in a difficult legal position. Although the best interest of the patient was the clear impetus for the author's actions, certain legal decisions recently have placed physicians who would follow Wynia's lead in a precarious position. Take the case of Baby K, an anencephalic infant whom physicians wanted to remove from life ...Reference1. Wynia M. Do everything. Ann Intern Med. 1994; 121:77. Google Scholar2. Annas GJ. Asking the courts to set the standard of emergency care—the case of Baby K. N Engl J Med. 1994;330:1542-5. Google Scholar Author, Article, and Disclosure InformationAuthors: Ronald A. Fronduti PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byAnxieties as a Legal Impediment to the Doctor-Proxy Relationship 1 December 1994Volume 121, Issue 11Page: 900-901 ePublished: 15 August 2000 Issue Published: 1 December 1994 Copyright & PermissionsCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Sleep-wake disorders based on a polysomnographic diagnosis. A national cooperative study
Authors: R. M. Coleman
Journal: JAMA (1982)

Under the organizational aegis of Project Sleep and the Association of Sleep Disorders Centers (ASDC), nearly 5,000 patient records from 11 sleep-wake disorders clinics were analyzed in a cooperative study. These cases represented the diagnostic experience of each of these centers over a two-year period. Each patient underwent polysomnographic study, and his or her condition was diagnosed according to the ASDC classification system, a new, standardized nosology of sleep disorders medicine. The most common major diagnostic category was "disorders of excessive sleepiness (hypersomnia)," 42%; this was followed by "disorders of initiating and maintaining sleep (insomnia)," 26%; "penile tumescence evaluations for impotency," 17%; "parasomnias," 3%; and "disorders of the sleep-wake schedule," 2%. If the impotency evaluations performed in the sleep clinics are removed from the total, leaving only the population that was studied because of sleep complaints, the proportions of the diagnostic categories are hypersomnia, 51%; insomnia, 31%; parasomnias, 15%; and sleep-wake schedule disturbances, 3%. The most prevalent diagnoses in the hypersomnia category were sleep apnea (43%) and narcolepsy (25%). Psychiatric disorders (35%) comprised the most frequent group of insomnia diagnoses, though a variety of other disorders were common. The applications of these results for the practicing physician are discussed. (<i>JAMA</i>1982;247:997-1003)
Intermittent enteral feeding: the influence on respiratory and digestive tract colonization in mechanically ventilated intensive-care-unit patients.
Authors: M Metersky, D Skiest
Journal: American Journal of Respiratory and Critical Care Medicine (1997)

"Intermittent enteral feeding: the influence on respiratory and digestive tract colonization in mechanically ventilated intensive-care-unit patients.." American journal of respiratory and critical care medicine, 155(5), pp. 1817–1818