First detection of SARS-CoV-2 spike protein N501 mutation in Italy in August, 2020
Authors: Simona Fiorentini, Serena Messali, Alberto Zani, Francesca Caccuri, Marta Giovanetti, Massimo Ciccozzi, Arnaldo Caruso
Journal: The Lancet Infectious Diseases (2021)

A new variant of SARS-CoV-2, known as VOC-202012/01, is spreading in the UK and is rapidly becoming a global threat.1WHOWHO SARS-CoV-2 variant—United Kingdom of Great Britain and Northern Ireland.https://www.who.int/csr/don/21-december-2020-sars-cov2-variant-united-kingdom/enDate accessed: December 22, 2020Google Scholar, 2Rambaut A Loman N Pybus O et al.Preliminary genomic characterisation of an emergent SARS-CoV-2 lineage in the UK defined by a novel set of spike mutations.https://virological.org/t/preliminary-genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-the-uk-defined-by-a-novel-set-of-spike-mutations/563Date accessed: December 21, 2020Google Scholar VOC-202012/01 is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).3Yi C Sun X Ye J et al.Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies.Cell Mol Immunol. 2020; 17: 621-630Crossref PubMed Scopus (324) Google Scholar On Nov 10, 2020, a 59-year-old man with a history of SARS-CoV-2 infection persistence presented for molecular testing. Infection was laboratory confirmed; therefore, genetic characterisation of viruses detected in the sample collected in November (MB61-Nov) and in a previous sample collected in August (MB61-Aug) was done by metagenomic sequencing.4Caccuri F Zani A Messali S et al.A persistently replicating SARS-CoV-2 variant derived from an asymptomatic individual.J Transl Med. 2020; 18: 362Crossref PubMed Scopus (40) Google Scholar The two complete genomes were compared with full-length viral genomes available on GISAID (715 Italian isolates plus 1422 reference sequences representative of the global SARS-CoV-2 epidemic). EPI_ISL_717978 and EPI_ISL_728343 strains, carrying the mutation N501Y in the spike RBD, and recently characterised in Italy and in the UK as belonging to the rapidly emerging B.1.1.7 lineage, were also included in the analysis.2Rambaut A Loman N Pybus O et al.Preliminary genomic characterisation of an emergent SARS-CoV-2 lineage in the UK defined by a novel set of spike mutations.https://virological.org/t/preliminary-genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-the-uk-defined-by-a-novel-set-of-spike-mutations/563Date accessed: December 21, 2020Google Scholar, 5Tegally H Wilkinson E Giovanetti M et al.Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple spike mutations in South Africa.medRxiv. 2020; (published online Dec 22.) (preprint)https://doi.org/10.1101/2020.12.21.20248640Google Scholar Sequence alignment and editing were done as previously described.6Laiton-Donato K Villabona-Arenas CJ Usme-Ciro JA et al.Genomic epidemiology of severe acute respiratory syndrome coronavirus 2, Colombia.Emerg Infect Dis. 2020; 26: 2854-2862Crossref PubMed Google Scholar Bioinformatic analyses showed that the MB61-Aug SARS-CoV-2 isolate had accumulated ten amino acid changes compared with early Italian isolates, and three more had emerged along its evolution by the end of November (appendix). Phylogeny showed that the viral strains obtained from the patient belonged to the B.1.1 lineage and clustered together with strong support (bootstrap value 1.0). The time-stamped phylogeny was consistent with a persistent infection and accelerated viral evolution (appendix). Compared with the Wuhan reference strain, both MB61 variants showed four mutations and one deletion in the spike protein, two of which were located within the RBD (appendix). It is worth noting that the N501T substitution was detected in both MB61-Nov and MB61-Aug SARS-CoV-2 isolates, highlighting that a mutation at the critical amino acid residue 501 was already present in Italy in August, 2020. Our time-scaled maximum likelihood tree suggests that these spike N501T variants emerged in early August (95% highest posterior density early July to end of August) in northern Italy (appendix), and therefore that SARS-CoV-2 strains harbouring a substitution at position 501 might have circulated unnoticed even before the end of September, 2020, when the rapidly emerging B.1.1.7 lineage (carrying the N501Y mutation) was first reported.2Rambaut A Loman N Pybus O et al.Preliminary genomic characterisation of an emergent SARS-CoV-2 lineage in the UK defined by a novel set of spike mutations.https://virological.org/t/preliminary-genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-the-uk-defined-by-a-novel-set-of-spike-mutations/563Date accessed: December 21, 2020Google Scholar Interestingly, differently from VOC-202012/01, the MB61 variants showed a second mutation within the RBD at the amino acid position Q493K that, together with N501T, might alter the binding affinity of the spike protein to the ACE2 receptor. Recent findings about SARS-CoV-2 evolution, especially within the RBD, call for a massive effort in the scientific community to identify new variants that might increase viral spreading, as well as allow escape from natural or vaccine-induced neutralising immunity. We declare no competing interests. This work was supported by the University of Brescia research funds. Ethical approval was obtained from the Brescia Ethical Committee (number EvoSCONP4369). The patient has provided written informed consent for publication to the authors. Download .pdf (.42 MB) Help with pdf files Supplementary appendix