tbuckley/cabot-search huggingface dataset:
Respiratory disease, and treatment / thematic poster session development of a mask for bronchoscopy to prevent infection under COVID-19 pandemic
Authors: K. Sato, H. Yasui, K. Takahashi, S. Taki, T. Ito, S. Okachi
Journal: American Journal of Respiratory and Critical Care Medicine (2021)

Introduction: COVID-19 pandemic makes us realize that infectious disease a threat for humankind. Because respiratory infections such as coronaviruses are transmitted through contact and droplets, bronchoscopy is thought to be a high risk manipuration. An increased risk of virus transmission through droplets and aerosols has been reported, and strict infection control measures are essential. Healthcare providers take standard precautions, but few measures are taken on the side of patients who generates infectious droplets and aerosols.Therefore, innovative and user-friendly measures to cut droplets and aerosols are highly desirable.Method: To prevent droplets and aerosols during bronchoscopy, we created a simple, disposable, costeffective, mask for the patients who undergo bronchoscopy, with the Japanese mask industry help. The mask has a 10-mm-slit in the center for insertion of bronchoscope and a 6-mm-slit on both sides for suction catheter, which is realized with its unique pleats. The slits are closed with an electrified filter unless the tube is pierced. To evaluate the effectiveness of the prevention of droplet dispersal from the mouth, visualization of the particles with the super-highly sensitive camera and high-power light source (ViEST system) with or without the mask (bronchoscope and one suction catheter inserted) was performed and analyzed. With the quantification of droplets, and analysis of fluorescence-based image, the efficacy of prevention was evaluated. Moreover, we do the simulation with fluid dynamics software (Cradle) to visualize the spread of aerosol. The Aerosol was almost prevented with the mask. We also accessed the feasibility via the observation time of the bronchoscopy for the doctors. Results: The visualization and quantification of the droplets with ViEST system revealed that almost all the droplets are cut with the mask on bronchoscope. The simulation of the aerosol spreading indicated that the mask prevented the straightforward spreading of the aerosol from the patient's mouth. The observation time of bronchoscopy did not show the difference between with mask and without mask.In conclusion, this new mask might be useful in preventing splashes and aerosols during bronchoscopy. .
Adults Lag Behind Children in Reduced Head Injuries From Biking
Authors: Bridget M. Kuehn
Journal: JAMA (2021)

Child and adolescent emergency department (ED) visits for biking-related traumatic brain injuries dropped by almost 50% between 2009 and 2018, a CDC study reported. Despite the decline, a much smaller 5.5% reduction among adults points to a need for improved safety measures.
Abstract 458: Novel Interactions With AMP-activated Protein Kinase Identified by Promiscuous Biotin Ligase Assay
Authors: Yulia Lipovka, John P Konhilas
Journal: Circulation Research (2016)

Estrogen is positioned to play a unique role in cardiovascular disease (CVD) since it can respond to environmental, genetic and non-genetic cues to impact gene expression and cellular signaling. Recently AMP-activated protein kinase (AMPK) has been identified as an important modulator of CVD. We propose that AMPK represents a nodal point for estrogen-dependent modulation of CVD. Our preliminary studies in a non-muscle cell line demonstrate that estrogen (E2) regulates AMPK activity predominantly through Estrogen Receptor alpha (ERα). In addition, both classical estrogen receptors (ERα and ERβ) bind to the α-catalytic subunit of AMPK, and the upstream kinase complex LKB1 is required for AMPK activation after E2 stimulation. The next step is to translate these findings into the cardiac system. In order to do that, we validated a novel technology to screen for interactions based on a promiscuous biotin ligase from E. coli . We created vectors containing our protein of interest, AMPK and/or the upstream kinase complex, LKB1, fused to the biotin ligase. After infecting Neonatal Rat Cardiomyocytes (NRCM) with viruses expressing those constructs, we added biotin to the cells, allowing biotinylation of proximal endogenous proteins; following biotin-affinity capture and western blot analysis. We show that estrogen receptors bind both to the α-catalytic subunit of AMPK, and the upstream kinase complex LKB1 in NRCM. Co-immunoprecipitation of endogenous proteins confirmed those interactions. In addition, we characterized a novel interaction between AMPK and the cytoskeletal protein, vinculin. Previous studies in our lab showed that AMPK is a modulator of myofilament function, and that the estrogen receptors associate with myofilament proteins. These, along with the findings detailed above open a new perspective on cardiac energy regulation.
Comparing the clinical differences in white and black women with frontal fibrosing alopecia
Authors: P. Adotama, V. Callender, A. Kolla, C. Young, P. Jones, K. Svigos, L. Yin, N. Ugonabo, D. Gutierrez, E. Peterson, K. Lo Sicco, J. Shapiro
Journal: British Journal of Dermatology (2021)
Pediatric COVID-19 Disparities and Prioritizing Equity—Children Are Not Spared
Authors: Evida A. Dennis-Heyward, Snehal N. Shah
Journal: JAMA Pediatrics (2021)

Defne Saatci, MD; Tom A. Ranger, PhD; Cesar Garriga, PhD; Ash Kieran Clift, MD; Francesco Zaccardi, PhD; Pui San Tan, PhD; Martina Patone, PhD; Carol Coupland, PhD; Anthony Harnden, MD; Simon J. Griffin, MD; Kamlesh Khunti, PhD; Hajira Dambha-Miller, MD; Julia Hippisley-Cox, MD
Inhaled treprostinil and forced vital capacity in patients with interstitial lung disease and associated pulmonary hypertension: a post-hoc analysis of the INCREASE study
Authors: Steven D Nathan, Aaron Waxman, Sudarshan Rajagopal, Amy Case, Shilpa Johri, Hilary DuBrock, David J De La Zerda, Sandeep Sahay, Christopher King, Lana Melendres-Groves, Peter Smith, Eric Shen, Lisa D Edwards, Andrew Nelsen, Victor F Tapson
Journal: The Lancet Respiratory Medicine (2021)
Flexible Ultrasonic Patch for Accelerating Chronic Wound Healing
Authors: Wenhan Lyu, Yinji Ma, Siyu Chen, Haibo Li, Peng Wang, Ying Chen, Xue Feng
Journal: Advanced Healthcare Materials (2021)

Abstract Ultrasound treatment is an effective method for accelerating chronic wound healing. However, it is not widely used because traditional ultrasonic probes cannot be conformal to the wound surface, which leads to limitations of use and unstable treatment effects. In addition, the use of liquid coupling agent increases the chance of wound infection. A strategy is proposed to design and fabricate a flexible ultrasonic patch for treating chronic wounds effectively. The piezoelectric ceramic in the patch is discretized into several linearly arranged units, which are integrated on a flexible circuit substrate. A thin hydrogel patch is used as both encapsulation and coupling layer to avoid wound infection and ensure the penetration of ultrasound. The ultrasonic patch is soft, light, and can completely conform to the treatment area. Bending of the patch focuses the sound beams on the center of the bending circle, which achieves control of the target treatment area. Ultrasound treatment experiments are carried out on some type‐II diabetic rats. Immunohistochemical (IHC) results indicate that ultrasound accelerates wound healing by activating Rac1 in both dermal and epidermal layers. Treatment results show that wound treated with the ultrasound heals faster than wounds without. The healing time is shortened by ≈40%.
SARS-CoV-2 viremia is associated with distinct proteomic pathways and predicts COVID-19 outcomes
Authors: Yijia Li, Alexis M. Schneider, Arnav Mehta, Moshe Sade-Feldman, Kyle R. Kays, Matteo Gentili, Nicole C. Charland, Anna L.K. Gonye, Irena Gushterova, Hargun K. Khanna, Thomas J. LaSalle, Kendall M. Lavin-Parsons, Brendan M. Lilley, Carl L. Lodenstein, Kasidet Manakongtreecheep, Justin D. Margolin, Brenna N. McKaig, Blair A. Parry, Maricarmen Rojas-Lopez, Brian C. Russo, Nihaarika Sharma, Jessica Tantivit, Molly F. Thomas, James Regan, James P. Flynn, Alexandra-Chloé Villani, Nir Hacohen, Marcia B. Goldberg, Michael R. Filbin, Jonathan Z. Li
Journal: Journal of Clinical Investigation (2021)

BACKGROUND. SARS-CoV-2 plasma viremia has been associated with severe disease and death in COVID-19 in small-scale cohort studies. The mechanisms behind this association remain elusive.
Abstract 14711: Predictors and Prognostic Impact of Left Ventricular Function Improvement Following Percutaneous Coronary Intervention in Patients With Ischemic Cardiomyopathy
Authors: Ryosuke Murai, Reo Hata, Kazuki Matsushita, Akimune Kuwayama, Masanobu Ohya, Takenobu Shimada, Katsuya Miura, Hidewo Amano, Shunsuke Kubo, Koshi Miyake, Suguru Otsuru, Yuichi Kawase, Seiji Habara, Takeshi Tada, Hiroyuki Tanaka, Yasushi Fuku, Harumi Katoh, Tsuyoshi Goto, Kazushige Kadota
Journal: Circulation (2018)

Introduction: Predictors and clinical effects of left ventricular (LV) function recovery in ischemic cardiomyopathy (ICM) patients undergoing percutaneous coronary intervention (PCI) are uncertain....
A retrospective cohort study of risk factors for mortality among nursing homes exposed to COVID-19 in Spain
Authors: Clara Suñer, Dan Ouchi, Miquel Àngel Mas, Rosa Lopez Alarcon, Mireia Massot Mesquida, Núria Prat, Josep Maria Bonet-Simó, Marta Expósito Izquierdo, Irene Garcia Sánchez, Sara Rodoreda Noguerola, Montserrat Teixidó Colet, Joaquim Verdaguer Puigvendrelló, Norma Henríquez, Ramón Miralles, Eugènia Negredo, Marc Noguera-Julian, Michael Marks, Oriol Estrada, Jordi Ara, Oriol Mitjà
Journal: Nature Aging (2021)

Long-term care (LTC) facilities have shown remarkably high mortality rates during the coronavirus disease 2019 (COVID-19) outbreak in many countries1, and different risk factors for mortality have been identified in this setting2–5. Using facilities as the unit of analysis, we investigated multiple variables covering facility characteristics and socioeconomic characteristics of the geographic location to identify risk factors for excess mortality from a comprehensive perspective. Furthermore, we used a clustering approach to detect patterns in datasets and generate hypotheses regarding potential relationships between types of nursing homes and mortality trends. Our retrospective analysis included 167 nursing homes providing LTC to 8,716 residents during the COVID-19 outbreak in Catalonia (northeast Spain). According to multiple regression analysis, COVID-19-related and overall mortality at the facility level were significantly associated with a higher percentage of patients with complex diseases, lower scores on pandemic preparedness measures and higher population incidence of COVID-19 in the surrounding population. When grouping nursing homes into eight clusters based on common features, we found higher mortality rates in four clusters, mainly characterized by a higher proportion of residents with complex chronic conditions or advanced diseases, lower scores on pandemic preparedness, being located in rural areas and larger capacity, respectively. Facility-level factors associated with increased mortality rates among nursing home residents in Spain during the COVID-19 pandemic included a higher proportion of patients with complex diseases, lower scores on pandemic preparedness measures and higher COVID-19 incidence levels in the surrounding neighborhood.
Characterizing Concomitant Follicular Lymphoma and Gastric Carcinoma Using 68Ga-FAPI-04 and 18F-FDG PET/CT
Authors: Xuetao Chen, Maomao Wei, Shuailiang Wang, Zhi Yang, Xuejuan Wang
Journal: Clinical Nuclear Medicine (2021)

Abstract We herein report a case involving a 67-year-old man with concomitant progressive follicular lymphoma and gastric carcinoma. Baseline 18 F-FDG PET/CT showed high metabolic activity in multiple nodal stations and a thickened gastric antrum wall, whereas 68 Ga-FAPI-04 PET/CT depicted very intense tracer uptake in the gastric lesion but mild uptake in the nodes. After the treatment, complete remission from lymphadenopathy was achieved, whereas the gastric lesion accumulated more radiotracers compared with baseline levels. Despite our incorrect initial assumption of B-cell transformation, molecular imaging was able to profile the characteristics of these 2 diseases.
Respiratory disease, and treatment / thematic poster session outcomes of percutaneous tracheostomy for prolonged respiratory failure in sars-cov-2 infection
Authors: J. Arnold, C. A. Gao, E. Malsin, K. Todd, J. M. Coleman, M. Prickett, M. J. Cuttica, C. Argento, S. B. Smith
Journal: American Journal of Respiratory and Critical Care Medicine (2021)

Rationale: Patients with severe respiratory failure from SARS-CoV-2 often require prolonged mechanical ventilation. Tracheostomy has been described in these patients, but data are limited regarding the outcomes of patients with SARS-CoV-2 who require tracheostomy. Methods: We performed a retrospective review of patients with severe SARS-CoV-2 intubated at our tertiary care center from March to November 2020. We included those who had percutaneous tracheostomy for prolonged respiratory failure. We catalogued demographics and outcomes. Non-parametric data were compared using unpaired, two-tailed Mann-Whitney tests. Results: We identified 50 patients with prolonged respiratory failure from SARS-CoV-2 who had percutaneous tracheostomy. Median age was 65.5 (interquartile range, IQR 56 - 71) years, 64% were male, and median BMI was 28 (25 - 35). Median time to last follow-up was 61 (IQR 14 - 136) days, and 70% of patients were alive at last follow-up. Median time from intubation to tracheostomy was 19 (IQR 15 - 24) days. The majority of tube sizes were extended length Shiley size 6 (64%). Median time from tracheostomy to end of mechanical ventilation was 13 (IQR 8 - 20) days, and median time to decannulation was 24 (19 - 38) days. Seventeen patients (34%) were decannulated by hospital discharge, and 33 patients (66%) were decannulated by last follow-up;94% of survivors were decannulated by last follow-up. Two patients (4%) developed tracheal stenosis after decannulation. Patients who were decannulated were younger (56 (37 - 64) vs 69 (64 - 74) years, p<0.01). Providers involved in the tracheostomy procedures reported no SARS-CoV-2 infections amongst themselves. Conclusions: In this limited cohort of patients who had tracheostomy for prolonged respiratory failure from SARS-CoV-2, there was a high rate of decannulation. The majority of patients were decannulated by last followup, and almost all survivors were decannulated. This appears to be a unique feature of SARS-CoV-2, as the incidence of decannulation for other types of ARDS are lower. Continued study is needed to understand the long-term outcomes of survivors of SARS-CoV-2. .
원저 : Non - B , Non - C 간세포암종의 임상적 특징과 B 형 간염바이러스 , C 형 간염바이러스 및 TT Virus 바이러스혈증의 빈도
Authors: 조현호(Hyun Ho Cho), 김영호(Young Ho Kim), 정진모(Jin Mo Jung), 조광희(Kwang Hee Cho), 조상형(Sang Hyung Cho), 최대현(Dae Hyun Choi), 정숙향(Sook Hyang Jeong), 이진혁(Jin Hyuk Lee), 한철주(Chul Ju Han), 김유철(You Cheoul Kim), 이진오(Jhin Oh Lee), 김창민(Chang Min Kim)
Journal: Clinical and Molecular Hepatology (2001)

Background/Aims: About 15% of Korean hepatocellular carcinoma(HCC) are negative both of Hepatitis B surface antigen (HBsAg) and anti-hepatitis C virus (anti-HCV)in their sera. They can be classified as a non-B, non-C hepatocellular carcinoma group(NBNC group). The aims of our study were, firstly, to describe the clinical characteristics of Korean NBNC HCC and compare them with those of HBsAg-positive HCC(HBV group) and anti-HCV-positive HVV(HCV group). Secondly we wanted to assess the frequency of viremia of HBV, HCV and transfusion-transmitted virus(TTV)in NBNC HCC patients. Methods : we prospectively collected clinical data and sera from 113 NBNC HCC patients and performed PCR for HBV DNA, HCV RNA and TTV DNA. We also collected clinical data from 125 HBsAg-positive HCC patients during a similar period. Results : The mean age of the NBNC HCC group was 59 years, in-between that of the HBV and the HCV groups. A History of heavy alcohol drinking was found in 48% of the NBNC HCC group. This was significantly higher than that of the HBV group, but similar to that of the HCV group. Serum α FP level in the NBNC HCC group was more frequently in the normal range compared to that in the HBV and HCV groups. The detection rates of HBV DNA, HCV RNA and TTV DNA in the NBNC HCC group were 17%, 13% and 67% respectively. Conclusions : The NBNC HCC patients seemed to comprise a heterogeneous group of various etiologies and clinical presentations. About one third of these patients displayed evidence of viremia of HBV or HCV.
セロトニン神経伝達物質は腸内病原菌の病原性を調節する【JST・京大機械翻訳】
Authors: Kumar Aman, M Russell Regan, Pifer Reed, Menezes-Garcia Zelia, Cuesta Santiago, Narayanan Sanjeev, B MacMillan John, Sperandio Vanessa
Journal: Cell Host & Microbe (2020)
Abstract 323: Loss of Gravity Impairs Cardiac Neural Crest Cell Lineage Development and Function
Authors: Konstantinos E Hatzistergos, Krystalenia Valasaki, Zhijie Jiang, Lauro M Takeuchi, Wayne Balkan, Dieter Saur, Barbara Seidler, Darcy L DiFede, Joshua M Hare
Journal: Circulation Research (2016)

Introduction: A multitude of structural, haemodynamic and electromechanical cardiovascular disorders have been observed in humans following space-travel. These abnormalities are thought to emerge from transient alterations in autonomic nervous system (ANS). However, since the ANS is cardiac neural crest (CNC)-derived, whether microgravity-induced cardiomyopathies reflect CNC dysfunction, is unknown. Hypothesis: Impairment of CNCs underlies microgravity-induced cardiomyopathies. Methods: Myocardial explants from adult cKit CreERT2/+ ;IRG mice (n=5/group), as well as cKit CreERT2/+ ;IRG- derived (iPSC Kit-Cre ; n=6/group) and Wnt1-Cre;tdTomato -derived (iPSC Wnt1-Cre ; n=18/group) induced pluripotent stem cells, were cultured under static (SC) or simulated microgravity conditions (rotary cell-culture system; RCCS). Results: CNC lineage-tracing in cardiac explants illustrated that, compared to SC, RCCS abolished the pool of cKit + CNCs in adult hearts, indicated by quantitation of cKit CreERT2 - mediated EGFP expression ( p &lt;0.05). Cardiogenesis modeling experiments with iPSC Kit-Cre yielded fewer beating EBs ( p =0.0005), and ~10-fold reduction in EGFP + cardiomyocytes ( p =0.01), in RCCS vs . SC. Microarray analyses suggested that RCCS-mediated alterations in BMP and Wnt/β-catenin pathways, downregulated ANS and CNC-related gene programs, and enhanced vasculogenic differentiation without affecting the expression of cardiac mesoderm-related genes. Differences were verified by quantitative PCR. Modeling CNC development in iPSC Wnt1-Cre further confirmed an RCCS-mediated dramatic impairment in development and function of CNCs, indicated by quantitation of tdTomato expression in day-10 and day-21 beating embryoid bodies ( p &lt;0.0001). Intriguingly, the effect of RCCS in CNCs could be only partially rescued upon transfer to SC. Conclusions: Together these data indicate that microgravity negatively regulates the development and function of CNCs, thus partly explaining the cellular and molecular mechanisms of microgravity-induced cardiomyopathies. Moreover, these findings are expected to have important implications in space exploration, since they suggest an essential role for gravity in vertebrate development.
Platelet Function Tests and Inflammatory Markers for the Differentiation of Primary Thrombocytosis and Secondary Thrombocytosis
Authors: Piyapong Kanya, Ekarat Rattarittamrong, Ornkamon Wongtagan, Thanawat Rattanathammethee, Sasinee Hantrakool, Chatree Chai-Adisaksopha, Adisak Tantiworawit, Lalita Norasetthada
Journal: Blood (2017)
Validation of the STAR-CAP Clinical Prognostic System for Predicting Biochemical Recurrence, Metastasis, and Cancer-specific Mortality After Radical Prostatectomy in a European Cohort
Authors: Christoph Würnschimmel, Randi Marisa Pose, Mike Wenzel, Zhe Tian, Reha-Baris Incesu, Pierre Karakiewicz, Markus Graefen, Derya Tilki
Journal: European Urology (2021)
Clinical efficacy of T-cell therapy after short-term BRAF-inhibitor priming in patients with checkpoint inhibitor-resistant metastatic melanoma
Authors: Troels Holz Borch, Katja Harbst, Aynal Haque Rana, Rikke Andersen, Evelina Martinenaite, Per Kongsted, Magnus Pedersen, Morten Nielsen, Julie Westerlin Kjeldsen, Anders Handrup Kverneland, Martin Lauss, Lisbet Rosenkrantz Hölmich, Helle Hendel, Özcan Met, Göran Jönsson, Marco Donia, Inge Marie Svane
Journal: Journal for ImmunoTherapy of Cancer (2021)

Despite impressive response rates following adoptive transfer of autologous tumor-infiltrating lymphocytes (TILs) in patients with metastatic melanoma, improvement is needed to increase the efficacy and broaden the applicability of this treatment. We evaluated the use of vemurafenib, a small-molecule BRAF inhibitor with immunomodulatory properties, as priming before TIL harvest and adoptive T cell therapy in a phase I/II clinical trial. 12 patients were treated with vemurafenib for 7 days before tumor excision and during the following weeks until TIL infusion. TILs were grown from tumor fragments, expanded in vitro and reinfused to the patient preceded by a lymphodepleting chemotherapy regimen and followed by interleukin-2 infusion. Extensive immune monitoring, tumor profiling and T cell receptor sequencing were performed. No unexpected toxicity was observed, and treatment was well tolerated. Of 12 patients, 1 achieved a complete response, 8 achieved partial response and 3 achieved stable disease. A PR and the CR are ongoing for 23 and 43 months, respectively. In vitro anti-tumor reactivity was found in TILs from 10 patients, including all patients achieving objective response. Serum and tumor biomarker analyses indicate that baseline cytokine levels and the number of T cell clones may predict response to TIL therapy. Further, TCR sequencing suggested skewing of TCR repertoire during in vitro expansion, promoting certain low frequency clonotypes. Priming with vemurafenib before infusion of TILs was safe and feasible, and induced objective clinical responses in this cohort of patients with checkpoint inhibitor-resistant metastatic melanoma. In this trial, vemurafenib treatment seemed to decrease attrition and could be considered to bridge the waiting time while TILs are prepared.