tbuckley/cabot-search huggingface dataset:
Abstract 397: Differentiation and Maturation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in Nanopatterned Cell Culture
Authors: Yiqiang Zhang, Jong-Seob Choi, Alec Smith, Robb MacLellan, Deok-Ho Kim, Yiqiang Zhang
Journal: Circulation Research (2020)

Background: Human induced pluripotent stem cells (hiPSCs) are widely used in studies of developmental and regenerative biomedicine involving various cell types, including cardiomyocytes (CMs). Understanding the cellular and molecular processes during hiPSC-CM differentiation and maturation will be crucial to develop heart regeneration therapies. In addition, while bioengineered cellular cues have been shown to modulate cellular morphology and phenotypes, little is known regarding their effects on molecular and functional maturity of hiPSC-CMs. Aims: To determine the expression of cardiac and cell cycle genes and the electrophysiological properties of hiPSC-CMs during their differentiation and maturation on unpatterned (Flat) or isotropic/nanopatterned (Nano) cell culture surface. Methods and Results: Using small molecules, WTC-11 hiPSC were differentiated into CMs efficiently (92.5% TNNT2 + ; 95% NKX2-5 + ). The resultant hiPSC-CMs were re-plated in Flat or Nano surfaces and harvested at different time points. Cardiac genes Myh7, Tnnt2, Serca2a, Ryr2, Cacna1c , and Kcnj2 gradually and significantly increased during differentiation; this was accompanied by reduced expression of cell cycle genes. While myofilament genes expressions were similar between Nano and Flat cultured hiPSC-CMs, ion channel genes Scn5a, Cacna1c , and Kcnj2 were significantly higher in Nano group, suggesting that Nano cultured CMs were more matured. In addition, fewer hiPSC-CMs were proliferative (EdU + ) in 2-week Nano group compared to Flat group. This was associated with decreased expression of active cell cycle genes Ccne1, Cdk4, Cdk14, Ki67 and Plk1 in Nano 2-week CMs. Micro-electrode array (MEA) analysis demonstrated that Beat Period, Spike Amplitude, and Field Potential Duration were increased in the Nano group. Fluo-4 Ca 2+ imaging assay revealed improved Ca 2+ transition activities in isotropically cultured hiPSC-CMs. Conclusion: These results demonstrate a significant upregulation of cardiac genes along with a down-regulation of cell cycle genes during the differentiation and maturation of hiPSC-CM on Nano surfaces. Bioengineered nanotopographically patterned substrates promoted the maturation and electrophysiological functions of hiPSC-CMs.
Safety and Efficacy of Virus-Specific Cytotoxic T-Lymphocytes Manufactured By the IFN-g Cytokine Capture System for the Treatment of Refractory Adenovirus, Cytomegalovirus, Epstein Barr Virus, and BK Virus Infections in Children, Adolescents and Young Adults after Allogeneic Hematopoietic Stem Cell Transplantation, Solid Organ Transplantation, or with Primary Immunodeficiency (IND# 17449)
Authors: Allyson M. Flower, Rachel Friedmann, Janet Ayello, Olivia Rigot, Lauren Harrison, Erin Morris, Meghan Sturhahn, Elana Maryamchik, Yongping Wang, Lynn O'Donnell, Rolla Abu-Arja, Dean Anthony Lee, Julie-An Talano, Bryon Johnson, Nancy Bunin, Mitchell S Cairo
Journal: Blood (2020)
T-Cell Acute Lymphoblastic Leukemia Can be Subdivided into Six Genetically Distinct Subtypes with Prognostic Impact By Combination of Whole Genome and Whole Transcriptome Data
Authors: Janine Müller, Claudia Haferlach, Henning Ruge, Heiko Müller, Irene Fuhrmann, Manja Meggendorfer, Martha-Lena Müller, Wolfgang Kern, Torsten Haferlach, Anna Stengel
Journal: Blood (2020)
The Microbiome and Cancer: Creating Friendly Neighborhoods and Removing the Foes Within
Authors: Sheetal Parida, Dipali Sharma
Journal: Cancer Research (2020)

Abstract The human body is colonized by the microbial cells that are estimated to be as abundant as human cells, yet their genome is roughly 100 times the human genome, providing significantly more genetic diversity. The past decade has observed an explosion of interest in examining the existence of microbiota in the human body and understanding its role in various diseases including inflammatory bowel disease, neurologic diseases, cardiovascular disorders, and cancer. Many studies have demonstrated differential community composition between normal tissue and cancerous tissue, paving the way for investigations focused on deciphering the cause-and-effect relationships between specific microbes and initiation and progression of various cancers. Also, evolving are the strategies to alter tumor-associated dysbiosis and move it toward eubiosis with holistic approaches to change the entire neighborhood or to neutralize pathogenic strains. In this review, we discuss important pathogenic bacteria and the underlying mechanisms by which they affect cancer progression. We summarize key microbiota alterations observed in multiple tumor niches, their association with clinical stages, and their potential use in cancer diagnosis and management. Finally, we discuss microbiota-based therapeutic approaches.
Primary and Secondary Central Nervous System Lymphoma: Outcomes from Houston Methodist Cancer Center
Authors: Cesar Gentille Sanchez, Ethan Burns, Ibrahim Muhsen, Humaira Sarfraz, Carlo Guerrero, John T Rogers, Matthew D Cykowski, Joe Ensor, Kirk Heyne, Jorge G. Darcourt, Jasleen K. Randhawa, Shilpan S. Shah, Sai Ravi Kiran Pingali
Journal: Blood (2020)
Interim Analysis of Hematologic Malignancies (HM)-Screen-Japan 01: A Mutation Profiling Multicenter Study of Patients with AML
Authors: Kenichi Miyamoto, Kentaro Fukushima, SungGi Chi, Hirohiko Shibayama, Naoko Hosono, Takahiro Yamauchi, Seiichiro Katagiri, Akihiko Gotoh, Takanobu Morishita, Masamitsu Yanada, Kazuhito Yamamoto, Naohito Fujishima, Naoto Takahashi, Reiki Ogasawara, Takeshi Kondo, Yoshikazu Utsu, Nobuyuki Aotsuka, Kensuke Usuki, Takaaki ONO, Tsutomu Kobayashi, Junya Kuroda, Hiroto Horiguchi, Satoshi Iyama, Suguru Fukuhara, Koji Izutsu, Makoto Nakamura, Kensuke Kojima, Yosuke Minami
Journal: Blood (2020)
Phase 2 Study Evaluating the Efficacy and Safety of Parsaclisib in Patients with Relapsed or Refractory Marginal Zone Lymphoma (CITADEL-204)
Authors: Tycel J. Phillips, Paolo Corradini, Ronit Gurion, Caterina Patti, Monica Tani, Abraham Avigdor, Wojciech Jurczak, Amitkumar Mehta, Pier Luigi Zinzani, Izidore S. Lossos, Catherine Thieblemont, Fred Zheng, Erica Rappold, Wanying Zhao, Peter Johnson
Journal: Blood (2020)
An Anti-Bcma CAR T-Cell Therapy (C-CAR088) Shows Promising Safety and Efficacy Profile in Relapsed or Refractory Multiple Myeloma
Authors: Gang An, Weiwei Sui, Tingyu Wang, Xiaoyan Qu, Xian Zhang, Junfang Yang, Yan Zhang, Lu Zhang, Judy Zhu, Chengxiao Zheng, Xiuxiu Yan, Xiaoteng Lv, Liping Lan, Dingzhu Yang, Ningning Huo, Ting Han, Dijun Zhao, Shichao Qin, Junfeng Wu, Xin Yao, Shigui Zhu, Jiaqiang Ren, Li Zhang, Jiaqi Huang, Michael Humphries, Yihong Yao, Daobin Zhou, Jianyong Li, Peihua Lu
Journal: Blood (2020)
Safety Profile of Idelalisib in Patients with Refractory Follicular Lymphoma: Interim Analysis of a Noninterventional Study
Authors: Gilles Salles, Ka Lung Wu, Sofya Kovalchuk, Santiago Mercadal, Annalisa Chiarenza, Ruth Pettengell, Elisabeth Vandenberghe, Paolo Corradini, Susana Carvalho, Christophe De Belder, Rikisha Shah Gupta, Anna van Troostenburg, Nishanthan Rajakumaraswamy, Heribert Ramroth
Journal: Blood (2020)
Abstract 449: Myristic Acid Conjugated Protein Kinase C Beta II Peptide Inhibitor Elicits Robust Cardioprotective Effects in Rat Myocardial Ischemia-Reperfusion Injury
Authors: Alison M Baker, Melinda Beale, Daphne Metellus, Ian Madison, Sunit Singh, Jeremy Castro, Michael Lloyd, Redona Gjoka, Rose Martorana, Anahi McIntyre, Jennifer Dang, Annam Humayun, Tameka Dean, Qian Chen, Robert Barsotti, Lindon Young
Journal: Circulation Research (2020)

Reactive oxygen species (ROS) induced ischemia-reperfusion (I/R) injury is a phenomenon causing paradoxical myocardial damage after cardio-angioplasty, coronary bypass and organ transplantation. Previous studies show that a cell-permeable myristic acid (myr-) conjugated PKCβII peptide inhibitor given at reperfusion prevents PKCβII translocation (N - myr-SLNPEWNET; myr-PKCβII-) and significantly attenuates ROS mediated I/R injury. We included a scrambled myr-PKCβII- (N-myr-WNPESLNTE; myr-PKCβII-scram) to examine the effects of myr separately. We hypothesize that myr-PKCβII- will improve and myr-PKCβII activator peptide (N-myr-SVEIWD; myr-PKCβII+) will exacerbate infarct size and post-reperfused cardiac function compared to myr-PKCβII-scram and untreated controls. Hearts isolated from male Sprague-Dawley rats (~300g) were perfused with Krebs’ buffer at a constant pressure of 80mmHg and subjected to 30 min of global ischemia and 50 min reperfusion. Myr-PKCβII-, myr-PKCβII+, myr-PKCβII-scram (all 20μM), or untreated control were given during the first five minutes of reperfusion. Left ventricular (LV) dP/dt max and dP/dt min (mmHg/s) were measured using a pressure transducer, and infarct size was determined using 1% triphenyltetrazolium chloride staining comparing infarcted tissue vs. total tissue weight. Data were evaluated using ANOVA with Student-Newman-Keuls post-hoc analysis. Myr-PKCβII- (n=17) significantly improved LV dP/dt max and dP/dt min to 1535±107 and 1063±83 at 50 min post-reperfusion compared to untreated control (815±107 and 722±89; n=15); myr-PKCβII-scram (513±78 and 433±66; n=12), and myr-PKCβII+ (860±118 and 694±81; n=14) (all p&lt;0.05). Myr-PKCβII- significantly reduced infarct size (%) to 13±2 compared to untreated control (24±4); myr-PKCβII-scram (22±2), and myr-PKCβII+ (21±3) (all p&lt;0.05). Unexpectedly, myr-PKCβII-scram significantly depressed post-reperfused LV dP/dt max and LV dP/dt min compared to untreated control and other treated groups (p&lt;0.05). Results suggest that myr-PKCβII- exerted significant cardioprotective effects compared to untreated controls, myr- PKCβII+ and myr-PKCβII-scram and would improve clinical outcomes after cardio-angioplasty or organ transplantation.
Survival Implications of Opioid Use after Blood and Marrow Transplantation
Authors: Noha N. Soror, Ashleigh Keiter, Qiuhong Zhao, Julianna Roddy, Sam Penza, Jonathan E Brammer, Yvonne A. Efebera, Don M. Benson, Sumithra Vasu, Alice S. Mims, Bradley W Blaser, Hannah Choe, Karilyn Larkin, Nicole Grieselhuber, Sarah A Wall, Ayman Saad, Ashley Rosko, Patrick Elder, Maria Chaudhry, Naresh Bumma, Abdullah Khan, Srinivas Devarakonda, Samantha Jaglowski, Basem M. William
Journal: Blood (2020)
pH-Activatable cell penetrating peptide dimers for potent delivery of anticancer drug to triple-negative breast cancer
Authors: So Hee Nam, Joomyung Jang, Dae Hee Cheon, Seung-Eun Chong, Joon Hyung Ahn, Soonsil Hyun, Jaehoon Yu, Yan Lee
Journal: Journal of Controlled Release (2020)
Blast MRD AML-1 Trial: Blockade of PD-1 Added to Standard Therapy to Target Measurable Residual Disease in Acute Myeloid Leukemia (AML) 1- an Investigator-Initiated, CTEP-Sponsored, Randomized Phase 2 Study of the Anti-PD-1 Antibody Pembrolizumab in Combination with Conventional Intensive Chemotherapy (IC) As Frontline Therapy in Patients with Acute Myeloid Leukemia (AML)
Authors: Amer M. Zeidan, Prajwal C Boddu, Brent L Wood, Daniel Zelterman, Richard F. Little, S. Percy Ivy, Anne Caldwell, Beatriz Sanchez-Espiridion, Gheath Alatrash, Elad Sharon, Jerald P. Radich
Journal: Blood (2020)
Incidence of and Risk Factors for Venous Thromboembolism Among Hospitalized Patients with Cancer and COVID-19: Report from the COVID-19 and Cancer Consortium (CCC19) Registry
Authors: Ang Li, Nicole M. Kuderer, Jeremy L. Warner, Aakash Desai, Dimpy P. Shah, Julie Fu, Monica Li, Rebecca Zon, Surbhi Shah, Shuchi Gulati, Ali Raza Khaki, Amit Kulkarni, Amro Elshoury, Michael A. Thompson, Petros Grivas, Gilberto de Lima Lopes, Yu Shyr, Nathan A Pennell, Andrew Schmidt, Christopher R Friese, Daniel Stover, Jaymin Patel, Balazs Halmos, Toni K. Choueiri, Solange Peters, Donna R. Rivera, Corrie Painter, Brian Rini, Gary H. Lyman, Jean M. Connors, Rachel P. Rosovsky
Journal: Blood (2020)
A Novel Method for Molecular Enumeration of Circulating Tisa-Cel and Axi-Cel in Lymphoma Patients
Authors: Chiara Monfrini, Vanessa Aragona, Martina Magni, Riccardo Betta, Cristina Vella, Chiara Paolizzi, Anna Dodero, Annalisa Chiappella, Anna Guidetti, Paolo Corradini, Cristiana Carniti
Journal: Blood (2020)
Persistent Activation of JAK/STAT Signaling Plays an Important Role in<i>in Vitro</i>Jaki Resistance in<i>TYK2-</i>rearranged B-Cell Acute Lymphoblastic Leukaemia
Authors: Paniz Tavakoli, Laura N Eadie, Susan L Heatley, John B Bruning, Deborah L White
Journal: Blood (2020)
Zinc Deficiency–Associated Dermatitis
Authors: Sina D Schröder, Sebastian-Willibald Griebl
Journal: New England Journal of Medicine (2020)

A 4-month-old boy presented with a progressively worsening rash on his abdomen, diaper area, limbs, face, and scalp. A biopsy was performed, and a diagnosis of zinc deficiency–associated dermatitis was made.
Selinexor in Combination with Pomalidomide and Dexamethasone (SPd) for Treatment of Patients with Relapsed Refractory Multiple Myeloma (RRMM)
Authors: Christine I Chen, Nizar J. Bahlis, Cristina Gasparetto, Sascha A Tuchman, Brea Lipe, Muhamed Baljevic, Rami Kotb, Heather J Sutherland, William I Bensinger, Michael Sebag, Richard LeBlanc, Christopher P. Venner, Gary J. Schiller, Suzanne Lentzsch, Natalie S. Callander, Adriana C Rossi, Heidi Sheehan, Dane Van Domelen, Kai Kazuharu, Hongwei Wang, Jatin Shah, Sharon Shacham, Michael G Kauffman, Darrell J White
Journal: Blood (2020)
Cell senescence: basic mechanisms and the need for computational networks in vascular ageing
Authors: Véronique Regnault, Pascal Challande, Florence Pinet, Zhenlin Li, Patrick Lacolley
Journal: Cardiovascular Research (2020)

This review seeks to provide an update of the mechanisms of vascular cell senescence, from newly identified molecules to arterial ageing phenotypes, and finally to present a computational approach to connect these selected proteins in biological networks. We will discuss current key signalling and gene expression pathways by which these focus proteins and networks drive normal and accelerated vascular ageing. We also review the possibility that senolytic drugs, designed to restore normal cell differentiation and function, could effectively treat multiple age-related vascular diseases. Finally, we discuss how cell senescence is both a cause and a consequence of vascular ageing because of the possible feedback controls between identified networks.
Hepatitis G virus (HGC) infection and subtypes of GB virus C/HCV positive liver transplant recipients
Authors: L. M. M. B Pereira, R. M. C. Leitão, A. Ong Fernandez, P. C. B Massarolo, Sérgio Mies, I. G McFarlane, M Mizokami
Journal: Hepatology (1998)
Targeting Aberrant Chromatin in Chronic Lymphocytic Leukemia
Authors: Zhiquan Wang, Justin C. Boysen, Huihuang Yan, Charla R. Secreto, Sameer A. Parikh, Saad S. Kenderian, Wei Ding, Esteban Braggio, Susan L. Slager, Neil E. Kay
Journal: Blood (2020)
Abstract 272: Epigenetic Regulation Involved in Diabetes-induced Impairment in Myocardial Reparative Function of Endothelial Progenitor Cell-derived Exosomes
Authors: Grace Huang, Alycia Hildebrand, Cindy Benedict, Maria Cimini, Chunlin Wang, Zhongjian Cheng, Venkata N Garikipati, Vandana Mallaredy, Raj Kishore
Journal: Circulation Research (2020)

Myocardial infarction (MI) occurs frequently in patients with diabetes resulting in higher mortality and morbidity than non-diabetic patients. We and others have shown that bone marrow-derived endothelial progenitor cells (EPCs) promote cardiac neovascularization and attenuate ischemic injury in animal models. Moreover, emerging evidence supports that exosomes (Exo) mediate stem cell therapy by carrying cell-specific biological signatures and by inducing signaling via transfer of bioactive molecules to target cells. However, autologous cell-based therapies yielded modest clinical results, suggesting that cellular/Exo reparative function may be compromised on a background of disease such as diabetes. In addition, recent studies suggest epigenetic mechanisms, such as histone methylation for gene silencing, promotes diabetes-induced vascular complication. Therefore, we hypothesized that diabetic EPCs produce exosomes of altered and dysfunctional content which compromise EPC reparative function in ischemic heart disease via epigenetic alterations. We collected EPC-Exo from non-diabetic mice (Lepr db/+ ) and diabetic mice (Lepr db/db ) and examined their effect on tube formation and cardiomyocyte/endothelial cell survival in vitro as well as their reparative effects on permanent and acute ischemia/reperfusion (I/R) myocardial ischemic injuries in vivo . Diabetic EPC-Exo promoted neonatal rat cardiomyocyte cell apoptosis under hypoxic stress and repressed endothelial tube formation and cell survival compared to cells treated with WT EPC-Exo. In vivo studies revealed diabetic EPC-Exo significantly attenuated cardiac function, reduced capillary density, increased fibrosis and infarct size in permanent LAD ligation and I/R MI models. Mechanistically,H3K9Me3 was increased in mouse cardiac endothelial cells treated with diabetic EPC-exo, suggesting inhibition of angiogenic genes. Our results provide evidence that diabetic EPC-derived exosomes lose their cardiac reparative activities. Specific angiogenic genes will be examined by CHIP analysis of H3K9Me3. Reversing EPC-Exo function by manipulating H3K9Me3 expression will augment autologous therapies in regenerative medicine.
EOS789, a broad-spectrum inhibitor of phosphate transport, is safe with an indication of efficacy in a phase 1b randomized crossover trial in hemodialysis patients
Authors: Kathleen M. Hill Gallant, Elizabeth R. Stremke, Laurie L. Trevino, Ranjani N. Moorthi, Simit Doshi, Meryl E. Wastney, Nozomi Hisada, Jotaro Sato, Yoshitaka Ogita, Naohisa Fujii, Yuya Matsuda, Takei Kake, Sharon M. Moe
Journal: Kidney International (2020)
Molecular machineries and physiological relevance of ER-mediated membrane contacts
Authors: Shiyin Lin, Tian Meng, Haofeng Huang, Haixia Zhuang, Zhengjie He, Huan Yang, Du Feng
Journal: Theranostics (2020)

Membrane contact sites (MCSs) are defined as regions where two organelles are closely apposed, and most MCSs associated with each other via protein-protein or protein-lipid interactions. A number of key molecular machinery systems participate in mediating substance exchange and signal transduction, both of which are essential processes in terms of cellular physiology and pathophysiology. The endoplasmic reticulum (ER) is the largest reticulum network within the cell and has extensive communication with other cellular organelles, including the plasma membrane (PM), mitochondria, Golgi, endosomes and lipid droplets (LDs). The contacts and reactions between them are largely mediated by various protein tethers and lipids. Ions, lipids and even proteins can be transported between the ER and neighboring organelles or recruited to the contact site to exert their functions. This review focuses on the key molecules involved in the formation of different contact sites as well as their biological functions.
Mutational Patterns and Correlation to Chip-Related Mutations in Hematological Malignancies - a Study on Mutation Frequencies of 122 Genes in 28 Entities Including 3096 Cases
Authors: Anna Stengel, Constance Baer, Wencke Walter, Manja Meggendorfer, Wolfgang Kern, Torsten Haferlach, Claudia Haferlach
Journal: Blood (2020)
Oral Rilzabrutinib, a Bruton Tyrosine Kinase Inhibitor, Showed Clinically Active and Durable Platelet Responses and Was Well-Tolerated in Patients with Heavily Pretreated Immune Thrombocytopenia
Authors: David J. Kuter, Merlin Efraim, Jiri Mayer, Vickie McDonald, Robert J. Bird, Thomas Regenbogen, Mamta Garg, Zane Kaplan, Olga Bandman, Regan Burns, Ann Neale, Dolca Thomas, Nichola Cooper
Journal: Blood (2020)
Urinary Magnesium in the Evaluation of Hypomagnesemia
Authors: Bryan M. Tucker, James L. Pirkle, Rajeev Raghavan
Journal: JAMA (2020)

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Leveraging Whole Genome Sequencing to Define the Mutational Landscape in Paroxysmal Nocturnal Hemoglobinuria
Authors: Adam Wahida, Stephan Hutter, Manja Meggendorfer, Cassandra M. Kerr, Carmelo Gurnari, Elisabeth Weiss, Claudia Haferlach, Wolfgang Kern, Jaroslaw P. Maciejewski, Torsten Haferlach
Journal: Blood (2020)
Abstract 226: Recellularization of Xenograft Heart Valves Reduces the Xenoreactive Immune Response in an <i>In Vivo</i> Rat Model
Authors: Sabin J Bozso, Ryaan EL-Andari, Lin Fu Zhu, Benjamin Adam, Michael C Moon, Darren H Freed, Jayan Nagendran, Jeevan Nagendran
Journal: Circulation Research (2020)

Background: Current xenograft valve constructs provoke an intense immune response that may lead to valve dysfunction. Our aim was to address the role of autologous mesenchymal stem cell (MSC) recellularization of xenogenic valves on the activation of the xenoreactive immune response in an in-vivo rat model. Methods: Explanted aortic valve constructs from female Hartley guinea pigs were procured and decellularized, followed by recellularization with syngeneic Sprague-Dawley rat MSCs. The recellularized aortic valve xenografts were then implanted into the infrarenal aorta of recipient female Sprague-Dawley rats. Grafts were implanted as either syngeneic grafts, non-decellularized (fresh), decellularized and recellularized xenografts. Rats were euthanized after 7-days, exsanguinated and the grafts explanted. Total serum immunoglobulin was quantified and histological analysis perfomed to analyze the immune response. Results: Overall survival to endpoint was significantly lower in the decellularized xenograft group (67%; 4/6), compared to fresh (100%; 6/6) and recellularized grafts (100%; 6/6). Similarly grafts in the decellularized group were more likely to have completely thrombosed (50%; 2/4), compared to fresh (33%; 2/6) and recellularized grafts (0%; 0/6). Decellularized guinea pig xenografts, when implanted into rats in-vivo , result in significantly reduced total serum immunoglobulin production and significantly reduced graft cellular infiltrate when compared to fresh xenografts. Moreover, when decellularized guinea pig xenografts were recellularized with syngeneic rat MSCs there was an additional decrease in total serum immunoglobulin production and graft cellular infiltrate when compared to both fresh and decellularized xenografts. Importantly, recellularized guinea pig xenografts had an equivalent total immunoglobulin production and graft cellular infiltrate when compared to syngeneic rat aortic valve controls. Conclusions: Autologous MSC recellularization of xenogenic valves reduces the xenoreactive immune response in an in-vivo rat model and may be an effective approach to decrease the progression of xenograft valve dysfunction.