tbuckley/cabot-search huggingface dataset:
Abstract 104: Activated Cardiac Fibroblast Can Deactivate to Become Resting Fibroblasts Upon Injury Resolution
Authors: Onur Kanisicak, Hadi Khalil, Jason Karch, Bryan Maliken, Xing Fu, Jeffery D Molkentin
Journal: Circulation Research (2017)

Resident cardiac fibroblasts (CFs) are potential therapeutic targets in treating heart failure given the prominent role that fibrosis plays in this disorder. CFs directly convert to myofibroblasts (MFs) with injury where they mediate both adaptive wound healing after acute myocardial infarction as well as long-standing fibrosis during chronic disease states. However, the fate of activated MFs after injury resolution remains poorly understood, in part because the field has lacked a definitive strategy for identifying and tracing MFs and CFs in vivo. To address this issue we recently generated a novel mouse model that permits lineage tracing of all activated MFs in the heart after injury or stress stimulation. MFs were lineage traced with a tamoxifen inducible periostin allele knockin of the MerCreMer cDNA (PostnMCM) in combination with a Rosa26-eGFP Cre-dependent reporter. PostnMCM x R26-eGFP mice were transiently injured with the combined infusion of angiotensin II and phenylephrine (Ang/PE) for 2 weeks, during which time tamoxifen was also given to permanently label all newly formed MFs with eGFP. Ang/PE infusion was then removed and mice were then allowed to recover for 4 weeks as the fibrotic response regressed. The data show that during the Ang/PE infusion period nearly all the eGFP+ periostin lineage-traced myofibroblasts were αSMA positive with and activated myofibroblast gene expression profile. However, when the fibrotic response regressed weeks later, numerous periostin-lineage traced eGFP+ cells remained in the heart and these cells showed a phenotypic and molecular reversion back to resident CFs with a loss of myofibroblast marker genes. These results suggest that CFs are very unique cell types that can differentiate to MFs then de-differentiate back into resident CFs.
Abstract PO-054: Correlation of hematological toxicity with the bone marrow radiation dose and volume during concurrent chemo radiation in patients with cervical cancer
Authors: Irappa Madabhavi, Ragavendra Sagar, Apurva Patel, Malay Sarkar
Journal: Clinical Cancer Research (2021)

Abstract Aims &amp; Objective: Hematological toxicity is common in patients with cervical cancer treated with concurrent chemo radiotherapy (CT-RT), so the purpose is to assess this hematological toxicity and correlate the toxicity with the dose and volume of bone marrow included in the field of radiation. Materials &amp; Methods: Twenty-five patients with histologically proven cervical cancer attending to our cancer center from July 2008-August 2009 were the subjects of this study. Patients were treated on 6 MV linear accelerator with a radical intent with concurrent chemotherapy using cisplatin 50 mg weekly. The planning CT was done for all the patients before the treatment and contouring of the pelvic bone marrow apart from other organs at risk was done. Hematological toxicity was assessed using RTOG common toxicity criteria weekly during and at 2 weeks after the completion of the treatment. Results: A total of 25 patients on CT-RT treatment were assessed. Sixteen patients were in locally advanced stage. The variation in HB, TLC, Platelets, and ANC counts from the baseline to 2 weeks after chemo radiotherapy were assessed. Grade II anemia was observed in 12 and Grade III in 2 patients. There were no toxicity as far as WBC and platelets were considered. There was also no correlation between the volume of bone marrow included in the field of irradiation and appearance of anemia. Conclusion: CT-RT for cervical cancer is safe and is associated with minimal hematological toxicity in the form of anemia. The toxicity is same for different volumes of bone marrow included in the field of irradiation with both conventional as well as 3DCRT technique. The toxicity observed is probably contributed by Cisplatin. Citation Format: Irappa Madabhavi, Ragavendra Sagar, Apurva Patel, Malay Sarkar. Correlation of hematological toxicity with the bone marrow radiation dose and volume during concurrent chemo radiation in patients with cervical cancer [abstract]. In: Proceedings of the AACR Virtual Special Conference on Radiation Science and Medicine; 2021 Mar 2-3. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(8_Suppl):Abstract nr PO-054.
Converging genetic and epigenetic drivers of paediatric acute lymphoblastic leukaemia identified by an information-theoretic analysis
Authors: Michael A. Koldobskiy, Garrett Jenkinson, Jordi Abante, Varenka A. Rodriguez DiBlasi, Weiqiang Zhou, Elisabet Pujadas, Adrian Idrizi, Rakel Tryggvadottir, Colin Callahan, Challice L. Bonifant, Karen R. Rabin, Patrick A. Brown, Hongkai Ji, John Goutsias, Andrew P. Feinberg
Journal: Nature Biomedical Engineering (2021)
Association of Preconception Paternal Alcohol Consumption With Increased Fetal Birth Defect Risk
Authors: Qiongjie Zhou, Liting Song, Jingqi Chen, Qiaomei Wang, Haiping Shen, Shikun Zhang, Xiaotian Li
Journal: JAMA Pediatrics (2021)

This cohort study investigates the association of paternal drinking before pregnancy with risk of fetal birth defect.
Treatment Patterns and Clinical Outcomes After the Introduction of the Medicare Sepsis Performance Measure (SEP-1)
Authors: Ian J. Barbash, Billie S. Davis, Jonathan G. Yabes, Chris W. Seymour, Derek C. Angus, Jeremy M. Kahn
Journal: Annals of Internal Medicine (2021)

Medicare requires that hospitals report on their adherence to the Severe Sepsis and Septic Shock Early Management Bundle (SEP-1).To evaluate the effect of SEP-1 on treatment patterns and patient outcomes.Longitudinal study of hospitals using repeated cross-sectional cohorts of patients.11 hospitals within an integrated health system.54 225 encounters between January 2013 and December 2017 for adults with sepsis who were hospitalized through the emergency department.Onset of the SEP-1 reporting requirement in October 2015.Changes in SEP-1-targeted processes, including antibiotic administration, lactate measurement, and fluid administration at 3 hours from sepsis onset; repeated lactate and vasopressor administration for hypotension within 6 hours of sepsis onset; and sepsis outcomes, including risk-adjusted intensive care unit (ICU) admission, in-hospital mortality, and home discharge among survivors.Two years after its implementation, SEP-1 was associated with variable changes in process measures, with the greatest effect being an increase in lactate measurement within 3 hours of sepsis onset (absolute increase, 23.7 percentage points [95% CI, 20.7 to 26.7 percentage points]; P < 0.001). There were small increases in antibiotic administration (absolute increase, 4.7 percentage points [CI, 1.9 to 7.6 percentage points]; P = 0.001) and fluid administration of 30 mL/kg of body weight within 3 hours of sepsis onset (absolute increase, 3.4 percentage points [CI, 1.5 to 5.2 percentage points]; P < 0.001). There was no change in vasopressor administration. There was a small increase in ICU admissions (absolute increase, 2.0 percentage points [CI, 0 to 4.0 percentage points]; P = 0.055) and no changes in mortality (absolute change, 0.1 percentage points [CI, -0.9 to 1.1 percentage points]; P = 0.87) or discharge to home.Data are from a single health system.Implementation of the SEP-1 mandatory reporting program was associated with variable changes in process measures, without improvements in clinical outcomes. Revising the measure may optimize its future effect.Agency for Healthcare Research and Quality.
SARS-CoV-2 infection in schools in a northern French city: a retrospective serological cohort study in an area of high transmission, France, January to April 2020
Authors: Arnaud Fontanet, Laura Tondeur, Rebecca Grant, Sarah Temmam, Yoann Madec, Thomas Bigot, Ludivine Grzelak, Isabelle Cailleau, Camille Besombes, Marie-Noëlle Ungeheuer, Charlotte Renaudat, Blanca Liliana Perlaza, Laurence Arowas, Nathalie Jolly, Sandrine Fernandes Pellerin, Lucie Kuhmel, Isabelle Staropoli, Christèle Huon, Kuang-Yu Chen, Bernadette Crescenzo-Chaigne, Sandie Munier, Pierre Charneau, Caroline Demeret, Timothée Bruel, Marc Eloit, Olivier Schwartz, Bruno Hoen
Journal: Eurosurveillance (2021)

Background Children’s role in SARS-CoV-2 epidemiology remains unclear. We investigated an initially unnoticed SARS-CoV-2 outbreak linked to schools in northern France, beginning as early as mid-January 2020. Aims This retrospective observational study documents the extent of SARS-CoV-2 transmission, linked to an affected high school (n = 664 participants) and primary schools (n = 1,340 study participants), in the context of unsuspected SARS-CoV-2 circulation and limited control measures. Methods Between 30 March and 30 April 2020, all school staff, as well as pupils and their parents and relatives were invited for SARS-CoV-2 antibody testing and to complete a questionnaire covering symptom history since 13 January 2020. Results In the high school, infection attack rates were 38.1% (91/239), 43.4% (23/53), and 59.3% (16/27), in pupils, teachers, and non-teaching staff respectively vs 10.1% (23/228) and 12.0% (14/117) in the pupils’ parents and relatives (p &lt; 0.001). Among the six primary schools, three children attending separate schools at the outbreak start, while symptomatic, might have introduced SARS-CoV-2 there, but symptomatic secondary cases related to them could not be definitely identified. In the primary schools overall, antibody prevalence in pupils sharing classes with symptomatic cases was higher than in pupils from other classes: 15/65 (23.1%) vs 30/445 (6.7%) (p &lt; 0.001). Among 46 SARS-CoV-2 seropositive pupils &lt; 12 years old, 20 were asymptomatic. Whether past HKU1 and OC43 seasonal coronavirus infection protected against SARS-CoV-2 infection in 6–11 year olds could not be inferred. Conclusions Viral circulation can occur in high and primary schools so keeping them open requires consideration of appropriate control measures and enhanced surveillance.
Potential Value of Maternal Oxygen Supplementation—Reply
Authors: Nandini Raghuraman, Methodius G. Tuuli
Journal: JAMA Pediatrics (2021)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Abstract 15183: Thrombopoietin Has a Protective Effect Against Apoptosis of Endothelial Cells Through Activating Pi3k/akt Pathway
Authors: Liang Li, Junyan Wang, kuan zeng, hua zhang, Yanqi Yang, Mo Yang
Journal: Circulation (2020)

Introduction: Thrombopoietin (TPO) is a hematopoietic growth factor for platelet lineage. TPO was found to be neuroprotective in hypoxic-ischemic neonatal rat brain models and treatment with TPO reduced brain damage and improved sensorimotor functions (Li L et al, Aging-US, 2020). However, the underlying mechanism of TPO in this model, and its role in neural cells and endothelial cells were still unclear. Methods: C17.2 cells were divided into control (0.5% FCS), Normal (10% FCS), TPO and TPO + LY294002 groups. Human umbilical vein endothelial (HUVEC) cells were divided in to normal, CoCl 2 , TPO and TPO + CoCl2 groups. The expressed of TPO and c-mpl was tested by RT-PCR. The cell viability and apoptosis of each group were tested by Cell Counter Kit 8 (CCK-8) assay and flow cytometry. The expression of Caspase-3 and mitochondrial membrane potential (MMP) were then determined by flow cytometry with Caspase-3-PE and JC-1. The effect of TPO in PI3K/AKT pathway was detected by using Western blot. Results: Both TPO and c-mpl are expressed in the neurons of the human CNS. TPO was also detected in human cerebrospinal fluid. TPO promoted C17.2 cell proliferation through activation of the PI3K/Akt signaling pathway. Via the Bcl-2/BAX signaling pathway, TPO exerted an anti-apoptotic effect by suppressing mitochondria membrane potentials. We also investigated the protective effect of TPO on human endothelial cells. CoCl 2 significantly inhibited the growth of HUVECs. The cell viability of HUVECs decreased gradually with the enhancement of CoCl2 at a gradient of chemical concentrations (r= -0.997). CoCl 2 dramatically increased apoptosis of HUVECs, whereas pre-treatment with TPO rescued cell apoptosis induced by CoCl 2 (P&lt;0.001). Further investigation found that TPO decreased the expression of Caspase-3 and inhibited the reduction of MMP induced by CoCl 2 (P&lt;0.05). TPO increased the activation of PI3K/AKT pathway in HUVECs. Conclusions: TPO has a protective effect against apoptosis of neural cells and endothelial cells through activating the PI3K/AKT pathway, thus decreasing the expression of apoptosis protease Caspase-3 and inhibiting the reduction of MMP.
Health of women and children is central to covid-19 recovery
Authors: Neena Modi, Mark Hanson
Journal: BMJ (2021)

We present a case that illustrates the fluctuations in calcium levels to be expected while managing an infant with maternal gestational diabetes mellitus who also develops subcutaneous fat necrosis (SCFN). There is initial hypocalcaemia due to functional hypoparathyroidism, requiring judicious calcium replacement. But with increased extrarenal production of 1,25-dihydroxyvitamin D due to granulomatous inflammation of subcutaneous adipose tissue, hypercalcaemia ensues. With a self-limiting course, SCFN of the newborn has an excellent prognosis and resolves spontaneously. However, aberrations in serum calcium levels can manifest in life-threatening complications and must hence be closely monitored.
Remodeling glycerophospholipids affects obesity-related insulin signaling in skeletal muscle
Authors: Michael J. Wolfgang
Journal: Journal of Clinical Investigation (2021)

It has long been known that fatty acids can either adversely or positively affect insulin signaling in skeletal muscle, depending on chain length or saturation, and can therefore be primary drivers of systemic insulin sensitivity. However, the detailed mechanisms linking fatty acids to insulin signaling in skeletal muscle have been elusive. In this issue of the JCI, Ferrara et al. suggest a model whereby membrane lipid remodeling mediates skeletal muscle insulin sensitivity. The authors demonstrate that membrane glycerophospholipid fatty acid remodeling by lysophosphatidylcholine acyltransferase 3 (LPCAT3) in skeletal muscle from subjects with obesity was induced, suppressing insulin signaling and glucose tolerance. Loss or gain of LPCAT3 function in mouse models showed that Lpcat3 was both required and sufficient for high-fat diet-induced muscle insulin resistance. These results suggest that the physiochemical properties of muscle cell membranes may drive insulin sensitivity and, therefore, systemic glucose intolerance.
Epidemiology, clinical characteristics, and virologic features of COVID-19 patients in Kazakhstan: A nation-wide retrospective cohort study
Authors: Sergey Yegorov, Maiya Goremykina, Raifa Ivanova, Sara V. Good, Dmitriy Babenko, Alexandr Shevtsov, Kelly S. MacDonald, Yersin Zhunussov
Journal: The Lancet Regional Health - Europe (2021)
The CD94/NKG2A inhibitory receptor educates uterine NK cells to optimize pregnancy outcomes in humans and mice
Authors: Norman Shreeve, Delphine Depierreux, Delia Hawkes, James A. Traherne, Ulla Sovio, Oisin Huhn, Jyothi Jayaraman, Amir Horowitz, Hormas Ghadially, John R.B. Perry, Ashley Moffett, John G. Sled, Andrew M. Sharkey, Francesco Colucci
Journal: Immunity (2021)

The conserved CD94/NKG2A inhibitory receptor is expressed by nearly all human and ∼50% of mouse uterine natural killer (uNK) cells. Binding human HLA-E and mouse Qa-1, NKG2A drives NK cell education, a process of unknown physiological importance influenced by HLA-B alleles. Here, we show that NKG2A genetic ablation in dams mated with wild-type males caused suboptimal maternal vascular responses in pregnancy, accompanied by perturbed placental gene expression, reduced fetal weight, greater rates of smaller fetuses with asymmetric growth, and abnormal brain development. These are features of the human syndrome pre-eclampsia. In a genome-wide association study of 7,219 pre-eclampsia cases, we found a 7% greater relative risk associated with the maternal HLA-B allele that does not favor NKG2A education. These results show that the maternal HLA-B→HLA-E→NKG2A pathway contributes to healthy pregnancy and may have repercussions on offspring health, thus establishing the physiological relevance for NK cell education. VIDEO ABSTRACT.
Microarray evaluation of allergen-specific IgE in eosinophilic granulomatosis with polyangiitis
Authors: Federica Bello, Federica Maritati, Antonella Radice, Renato Alberto Sinico, Giacomo Emmi, Augusto Vaglio
Journal: Annals of the Rheumatic Diseases (2021)

The pathogenesis of asthma and ear–nose–throat (ENT) manifestations in eosinophilic granulomatosis with polyangiitis (EGPA) is still poorly understood. Asthma is present in almost all patients with EGPA.1 Severe or uncontrolled asthma occurs in more than 40% of patients and its severity correlates with serum IgE (sIgE) levels.2 However, sIgE towards common allergens are detectable in less than one-third of patients with EGPA using conventional diagnostic tests.3 This suggests either that atopy is not a key pathogenic mechanism in EGPA or that uncommon antigens are involved. Our study assessed IgE specificity in EGPA using microarray technologies which have higher diagnostic reliability than traditional assays and offer a wider representation of the IgE repertoire.4 5 We measured sIgE towards 112 purified or biotechnologically produced allergenic molecules using the ImmunoCAP Immuno Solid-phase Allergen Chip (ISAC) (online supplemental methods). Results are reported in ISAC standardised units (ISU). The study population comprised 29 patients with EGPA, evaluated during active and inactive disease (patients’ characteristics are reported in the online supplemental table 1), 30 patients with atopic asthma, 31 with active anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) (20 with granulomatosis with polyangiitis and 11 with microscopic polyangiitis) and 30 healthy controls (online supplemental methods). Positive IgE (ISU>0.3) in at least 5% of the whole study population were detected for 35 allergen components. We assessed for each …
A Closer Look at Health Inequities Among Pregnant People With Unstable Housing
Authors: Lindsay K. Admon, Mishka Terplan, Tyler N. A. Winkelman
Journal: JAMA Network Open (2021)

Ayae Yamamoto, PhD; Lillian Gelberg, MD, MSPH; Jack Needleman, PhD; Gerald Kominski, PhD; Sitaram Vangala, MS; Atsushi Miyawaki, MD, PhD; Yusuke Tsugawa, MD, PhD
Pulse arrival time, a novel sleep cardiovascular marker: the multi-ethnic study of atherosclerosis
Authors: Younghoon Kwon, Christopher Wiles, B Eugene Parker, Brian R Clark, Min-Woong Sohn, Sara Mariani, Jin-Oh Hahn, David R Jacobs, James H Stein, Joao Lima, Vishesh Kapur, Andrew Wellman, Susan Redline, Ali Azarbarzin
Journal: Thorax (2021)

Pulse arrival time (PAT) is commonly used to estimate blood pressure response. We hypothesised that PAT response to obstructive respiratory events would be associated with increased cardiovascular risk in people with obstructive sleep apnoea.
Impact of January 2021 curfew measures on SARS-CoV-2 B.1.1.7 circulation in France
Authors: Laura Di Domenico, Chiara E Sabbatini, Giulia Pullano, Daniel Lévy-Bruhl, Vittoria Colizza
Journal: Eurosurveillance (2021)

Following the spread of the SARS-CoV-2 B.1.1.7 variant, social distancing was strengthened in France in January 2021. Using a two-strain mathematical model calibrated on genomic surveillance, we estimated that curfew measures allowed hospitalisations to plateau by decreasing transmission of the historical strains while B.1.1.7 continued to grow. School holidays appear to have further slowed down progression in February. Without progressively strengthened social distancing, a rapid surge of hospitalisations is expected, despite the foreseen increase in vaccination rhythm.
Considerations for Racial Diversity in the Cardiology Workforce in the United States of America
Authors: Amber E. Johnson, Mehret Birru Talabi, Eliana Bonifacino, Alison J. Culyba, Naudia L. Jonassaint, Melonie A. Nance, G. Sarah Napoé, Oladipupo Olafiranye, Sylvia Owusu-Ansah, Tomeka L. Suber
Journal: Journal of the American College of Cardiology (2021)
Nonprofit Advocacy at the American Heart Association: Creating a Visionary Way Forward at Our 40th Anniversary: An American Heart Association Policy Statement
Authors: Keith Churchwell, Elliott Antman, Jill Birnbaum, Nihar R. Desai, Robert A. Harrington, Emily J. Holubowich, Kendra N. Ivy, Mark Schoeberl, John J. Warner, Courtney J. White, Laurie P. Whitsel, Clyde Yancy
Journal: Circulation (2021)

In 2021, the American Heart Association celebrates its 40th anniversary in advocacy. This policy statement details the arc of the organization's nonpartisan, evidence-based, equity-focused approach to advocating for public policy change, highlighting key milestones and describing the core components of the association's capacity and activity at all levels of government. This policy statement presents a vision and strategic imperative for future American Heart Association advocacy efforts to inform and influence policy changes that advance equitable, impactful societal solutions that transform and improve cardiovascular health for everyone. The American Heart Association maintains accountability by measuring and evaluating the totality of this work and its impact on equitable health outcomes. The American Heart Association will apply these lessons to constantly refine its own strategic policy focus and advocacy efforts. The association will also serve as a resource and catalyst to other organizations working to engage and educate policy makers, partners, the media, and funders about the important role and contribution of public policy change to achieve shared goals.