tbuckley/cabot-search huggingface dataset:
A single dose of self-transcribing and replicating RNA-based SARS-CoV-2 vaccine produces protective adaptive immunity in mice
Authors: Ruklanthi de Alwis, Esther S. Gan, Shiwei Chen, Yan Shan Leong, Hwee Cheng Tan, Summer L. Zhang, Clement Yau, Jenny G.H. Low, Shirin Kalimuddin, Daiki Matsuda, Elizabeth C. Allen, Paula Hartman, Kyoung-Joo Jenny Park, Maher Alayyoubi, Hari Bhaskaran, Adrian Dukanovic, Yanjie Bao, Brenda Clemente, Jerel Vega, Scott Roberts, Jose A. Gonzalez, Marciano Sablad, Rodrigo Yelin, Wendy Taylor, Kiyoshi Tachikawa, Suezanne Parker, Priya Karmali, Jared Davis, Brian M. Sullivan, Sean M. Sullivan, Steve G. Hughes, Pad Chivukula, Eng Eong Ooi
Journal: Molecular Therapy (2021)

A self-transcribing and replicating RNA (STARR)-based vaccine (LUNAR-COV19) has been developed to prevent SARS-CoV-2 infection. The vaccine encodes an alphavirus-based replicon and the SARS-CoV-2 full-length spike glycoprotein. Translation of the replicon produces a replicase complex that amplifies and prolongs SARS-CoV-2 spike glycoprotein expression. A single prime vaccination in mice led to robust antibody responses, with neutralizing antibody titers increasing up to day 60. Activation of cell-mediated immunity produced a strong viral antigen-specific CD8+ T lymphocyte response. Assaying for intracellular cytokine staining for interferon (IFN)γ and interleukin-4 (IL-4)-positive CD4+ T helper (Th) lymphocytes as well as anti-spike glycoprotein immunoglobulin G (IgG)2a/IgG1 ratios supported a strong Th1-dominant immune response. Finally, single LUNAR-COV19 vaccination at both 2 μg and 10 μg doses completely protected human ACE2 transgenic mice from both mortality and even measurable infection following wild-type SARS-CoV-2 challenge. Our findings collectively suggest the potential of LUNAR-COV19 as a single-dose vaccine.
Incidental Pancreatic Adenocarcinoma: More Frequent Than Expected?
Authors: G Soy, I Olivas, A Bofill, L Escudé, O Sendino, G Fernández-Esparrach, JR Ayuso, E Vaquero, F Ausania, T Saurí, S Alós, M Cuatrecasas, F Balaguer, A Ginès
Journal: Endoscopy (2021)

Aims To describe the frequency and characteristics of incidental PAC sent to our Endoscopy Unit for cytological diagnosis by EUS FNA during the last year.
Inhaled nitric oxide reverses pulmonary vasoconstriction in the hypoxic and acidotic newborn lamb.
Authors: J DRoberts, T YChen, NKawai, JWain, PDupuy, AShimouchi, KBloch, DPolaner, W MZapol
Journal: Circulation Research (1993)

We determined whether inhaling low levels of nitric oxide (NO) gas could selectively reverse hypoxic pulmonary vasoconstriction in the near-term newborn lamb and whether vasodilation would be attenuated by respiratory acidosis. To examine the mechanism of air and NO-induced pulmonary vasodilation soon after birth, we measured plasma and lung cGMP levels in the newly ventilated fetal lamb. Breathing at FIO2 0.10 nearly doubled the pulmonary vascular resistance index in newborn lambs and decreased pulmonary blood flow primarily by reducing left-to-right blood flow through the ductus arteriosus. Inhaling 20 ppm NO at FIO2 0.10 completely reversed hypoxic pulmonary vasoconstriction within minutes. Maximum pulmonary vasodilation occurred during inhalation of > or = 80 ppm NO. Breathing 8% CO2 at FIO2 0.10 elevated the pulmonary vascular resistance index to a level similar to breathing at FIO2 0.10 without added CO2. Respiratory acidosis did not attenuate pulmonary vasodilation by inhaled NO. In none of our stu...
Protection of hypoxic myocardium by intracoronary administration of verapamil in open-chest dogs.
Authors: HMori, MNagata, TMiyazaki, KSakurai, SOgawa, SHattori, MTakahashi, YNakamura
Journal: Circulation (1984)

Hypoxic perfusion of the regional myocardium was performed in 34 open-chest dogs. In eight dogs in which the myocardium was perfused with original hypoxic Krebs-Henseleit (K-H) solution for 5 min (group I) there was a marked decrease in adenosine triphosphate content (2.03 +/- 0.44 mumol/g) and an increase in lactate content (8.65 +/- 2.26 mumol/g). In myocardium of nine dogs (group II) perfused with verapamil-containing (1.3 mg/dl) K-H solution and in that of nine dogs (group III) receiving Ca2+-free solution, the degrees of reduction in adenosine triphosphate (2.99 +/- 0.73 and 3.25 +/- 0.48 mumol/g, respectively) and of lactate accumulation (5.16 +/- 0.59 and 4.79 +/- 1.02 mumol/g, respectively) at 5 min were significantly less than in group I. Absence of statistically significant differences in hemodynamic parameters among these three groups indicates that the metabolic preservation in group II probably resulted from a direct effect of verapamil on the regional myocardium related to Ca2+ metabolism. H...
Endoscopic Submucosal Dissection (ESD) or Endoscopic Mucosal Resection (EMR) for Early Esophageal Cancers? Results from the Graphe Prospective Multicenter Study
Authors: N Chapelle, M Barret, A Berger, S Hakim, JB Chevaux, M Camus, V Prouvost, S Koch, E Metivier-Cesbron, M Pioche, M Wangermez, G Rahmi, J Privat, C Bossard, JF Mosnier, G Vanbiervliet
Journal: Endoscopy (2021)

Aims Esophageal cancer is associated with excellent prognosis when diagnosed and treated at an early stage. Therefore, widespread adoption of EMR and ESD is of pivotal importance but poorly assessed in routine practice. Our aim was to assess the efficacy of EMR and ESD in “real life” conditions.
Multimodal Use of Advanced Endoscopic Resection Techniques for Local Recurrent Colorectal Adenomas: A Single Center Prospective Study
Authors: S Ghersi, A Gazzola, P Apolito, S Landi, M La Marca, M Bassi, E Dabizzi, A Larocca, V Cennamo
Journal: Endoscopy (2021)

Aims Local recurrence occurs in up to 20 % of piecemeal endoscopic mucosal resection (EMR) and re-treatment can be technically challenging. Advanced endoscopic techniques, such as endoscopic submucosal dissection (ESD) and endoscopic full thickness resection (EFTR), alone or combined, allow deep en bloc large lesion removal, thus potentially achieving oncological radicality. Our study aims to assess efficacy and safety of ESD, EFTR and hybrid techniques (ESD+EFTR; ESD+EMR) for the treatment of recurrent polyps.
Patients with two types of atrioventricular junctional (AV nodal) reentrant tachycardia. Evidence that a common pathway of nodal tissue is not present above the reentrant circuit.
Authors: M AMcGuire, K CLau, D CJohnson, D ARichards, J BUther, D LRoss
Journal: Circulation (1991)

BACKGROUNDThe site of the reentrant circuit in atrioventricular (AV) junctional reentrant tachycardia has not been defined; in particular, the existence of a common pathway of AV nodal tissue above the reentrant circuit is controversial.METHODS AND RESULTSTwo types of AV junctional reentrant tachycardia were induced in each of three patients at electrophysiological study. In one type of tachycardia (anterior), the onset of atrial activity occurred from 0 to 12 msec before the onset of ventricular activation, and earliest atrial activity was recorded near the His bundle. In the second type of tachycardia (posterior), the ventriculoatrial intervals were longer (76-168 msec), and earliest atrial activity was recorded near the mouth of the coronary sinus. In individual patients, the two types of tachycardia had different cycle lengths. Posterior AV junctional reentrant tachycardia was not a fast-slow form of AV junctional reentry in at least two of the three patients. Surgical cure was attempted in two patien...
Vcp-Regulated Homologous Recombination Represents a New Druggable Vulnerability in Acute Myeloid Leukemia
Authors: Lina Benajiba, Nina Fenouille, Edyta Malolepsza, Jana M Ellegast, Gabriela Alexe, Yana Pikman, Frédéric Luciano, Ilene Galinsky, Daniel J. DeAngelo, Richard M. Stone, Patrick Auberger, Josee Guirouilh-Barbat, Bernard Lopez, Mehdi Khaled, Kasper Lage, Olivier Hermine, Michael Hemann, Alexandre Puissant, Kimberly Stegmaier
Journal: Blood (2017)
Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration
Authors: Xiao Zheng, Xiaorong Zhang, Yingting Wang, Yangxi Liu, Yining Pan, Yijia Li, Man Ji, Xueqin Zhao, Shengbin Huang, Qingqing Yao
Journal: Bioactive Materials (2021)

Large bone defect repair requires biomaterials that promote angiogenesis and osteogenesis. In present work, a nanoclay (Laponite, XLS)-functionalized 3D bioglass (BG) scaffold with hypoxia mimicking property was prepared by foam replication coupled with UV photopolymerization methods. Our data revealed that the incorporation of XLS can significantly promote the mechanical property of the scaffold and the osteogenic differentiation of human adipose mesenchymal stem cells (ADSCs) compared to the properties of the neat BG scaffold. Desferoxamine, a hypoxia mimicking agent, encourages bone regeneration via activating hypoxia-inducible factor-1 alpha (HIF-1α)-mediated angiogenesis. GelMA-DFO immobilization onto BG-XLS scaffold achieved sustained DFO release and inhibited DFO degradation. Furthermore, in vitro data demonstrated increased HIF-1α and vascular endothelial growth factor (VEGF) expressions on human adipose mesenchymal stem cells (ADSCs). Moreover, BG-XLS/GelMA-DFO scaffolds also significantly promoted the osteogenic differentiation of ADSCs. Most importantly, our in vivo data indicated BG-XLS/GelMA-DFO scaffolds strongly increased bone healing in a critical-sized mouse cranial bone defect model. Therefore, we developed a novel BG-XLS/GelMA-DFO scaffold which can not only induce the expression of VEGF, but also promote osteogenic differentiation of ADSCs to promote endogenous bone regeneration.
De subitaneis mortibus. XVII. Multifocal stenoses due to fibromuscular dysplasia of the sinus node artery.
Authors: T NJames, T KMarshall
Journal: Circulation (1976)

The sinus node artery was focally narrowed by fibromuscular dysplasia in two examples of sudden unexpected death reported here. Although both cases had additional histological abnormalities in the conduction system of the heart, the more striking feature was the focal fibromuscular dysplasia. These findings are discussed in relationship to a large number of similar examples of focal fibromuscular dysplastic narrowing of the sinus node artery observed in other victims of sudden unexpected death, considering some possible mechanisms for lethal electrical instability of the heart and also the possible pathogenesis of such fibromuscular dysplasia.
Side effects of therapy with prostaglandin E1 in infants with critical congenital heart disease.
Authors: A BLewis, M DFreed, M AHeymann, S LRoehl, R CKensey
Journal: Circulation (1981)

The case reports of 492 infants with critical congenital cardiac disease treated with prostaglandin E1 (PGE1) were reviewed to determine the nature and incidence of intercurrent medical events. Forty-three percent of the infants had at least one such event, but only half of these were related to PGE1 and the majority required only minor changes in management. Cardiovascular events were the most common (18% incidence), with cutaneous vasodilation and edema occurring more frequently during intraaortic infusion than during i.v. infusion. Central nervous system events were reported in 16% of the patients. Respiratory depression was reported in 12%, and was particularly common in infants weighing less than 2.0 kg at birth (42%). Hematologic, infectious and renal events appeared for the most part to be unrelated to PGE1. The overall mortality (excluding 19 patients with hypoplastic left-heart syndrome) was 31%; the mortality for the patients with critical coarctation or interruption of the aortic arch was nearl...
Influence of atrial systole on the Frank-Starling relation and the end-diastolic pressure-diameter relation of the left ventricle.
Authors: TLinderer, KChatterjee, W WParmley, R ESievers, S AGlantz, J VTyberg
Journal: Circulation (1983)

The influence of atrial systole on the left ventricular function curve (stroke volume vs end-diastolic pressure or end-diastolic diameter) and on the left ventricular end-diastolic pressure diameter relation was studied in nine anesthetized, open-chest dogs whose atrioventricular (AV) node had been completely blocked. Measurements were made during volume loading with the pericardium closed and opened and during alternate AV sequential pacing (to permit atrial contribution to ventricular filling) and AV simultaneous pacing (to prevent atrial contribution). When the pericardium was closed, withdrawal of the atrial contribution shifted the stroke-volume end-diastolic pressure relation downward, but did not shift the stroke volume-end-diastolic diameter relation, i.e., it reduced stroke volume for a given end-diastolic pressure according to a reduction in end-diastolic volume. The downward shift of the stroke volume-end-diastolic pressure relation was caused by an upward shift of the end-diastolic pressure-di...
Long-term prognostic importance of patency of the infarct-related coronary artery after thrombolytic therapy for acute myocardial infarction.
Authors: H DWhite, D BCross, J MElliott, R MNorris, T WYee
Journal: Circulation (1994)

BACKGROUNDAfter thrombolytic therapy, long-term patency of the infarct-related artery may reduce arrhythmias, limit ventricular dilatation, and provide collaterals to another infarct zone if further infarction occurs. However, independent long-term prognostic value of infarct artery patency has not been shown.METHODS AND RESULTSWe followed 312 patients with first myocardial infarction treated < 4 hours after pain onset with thrombolysis (streptokinase [n = 188] or recombinant tissue-type plasminogen activator [n = 124]). At 28 +/- 11 days, cardiac catheterization was performed. Flow of the infarct-related artery was assessed by the TIMI scoring system, and a scoring system relating coronary stenoses and flow to the amount of myocardium supplied was also used. Follow-up was for 39 +/- 13 months. Cardiac death occurred in 5.8% of patients, and there were two noncardiac deaths. Revascularization was performed in 11.5% of patients. On univariate and multivariate analysis, ventricular function (ejection fracti...
Electrophysiological and clinical observations in patients with alternating bundle branch block.
Authors: DWu, PDenes, R CDhingra, FAmat-Y-Leon, C RWyndham, RChuquimia, K MRosen
Journal: Circulation (1976)

Electrophysiological studies (His bundle recordings and atrial stimulation) were performed in nine patients who manifested periods of both right and left bundle branch block (RBBB and LBBB). In seven of the patients, alternating bundle branch block appeared to reflect intermittent or chronic bundle branch block superimposed on incomplete (but electrocardiographically complete) block of the contralateral bundle branch. In three of these seven, shift from one bundle branch block pattern to the other was associated with reproducible change in H-V (mean change 30 msec), and could be induced by alteration of cardiac rate with carotid massage, coupled atrial stimulation, and rapid atrial pacing. In one of the seven, RBBB with a P-R of 0.20 seconds preceded chronic LBBB with a P-R of 0.24 seconds, implying that RBBB had been incomplete. In three of the seven, although a definite mechanism of alternation could not be demonstrated, transient contralateral bundle branch block occurred superimposed on chronic ipsila...
Calcific deposits in stenotic mitral valves. Extent and relation to age, sex, degree of stenosis, cardiac rhythm, previous commissurotomy and left atrial body thrombus from study of 164 operatively-excised valves.
Authors: A SLachman, W CRoberts
Journal: Circulation (1978)

The presence or absence and the extent of calcific deposits in excised stenotic mitral valves was determined by radiographs of the excised valve in 164 patients aged 26 to 72 years. The extent of the mitral calcific deposits was determined by the percent of the valvular circumference containing the deposits = grade 0 (14 patients); grade I = less than 25% (43 patients); grade II = 25-50% (34 patients); grade III = 51-75% (39 patients); and grade IV = greater than 75% (34 patients). The amount of calcific deposits in the stenotic mitral valves correlated with sex and with the mean diastolic pressure gradient across the mitral valve (P less than 0.05), but it did not correlate with the patient's age, cardiac rhythm, pulmonary arterial or pulmonary arterial wedge pressure, previous mitral commissurotomy, presence of thrombus in the body of left atrium or the presence of disease of one or more other cardiac valves.
Ventricular tachycardia in a young population without overt heart disease.
Authors: B JDeal, S MMiller, DScagliotti, DPrechel, J LGallastegui, R JHariman
Journal: Circulation (1986)

Since 1974, 24 young patients presenting with ventricular tachycardia and without clinical evidence of heart disease were evaluated and followed. Sixteen patients (67%) were symptomatic. Clinical episodes of ventricular tachycardia were sustained in 18, incessant in four, and nonsustained in two patients. The rate of tachycardia ranged from 130 to 300 beats/min (mean = 200 beats/min). Subtle abnormalities of cardiac size or function were present at cardiac catheterization in 16 of 23 patients (70%). During electrophysiologic studies, spontaneous ventricular tachycardia was present in six patients. The clinical ventricular tachycardia was inducible by programmed stimulation in 13 of 18 patients. The site of origin of tachycardia based on endocardial mapping in 17 patients was the right ventricle in 14, the ventricular septum in one, and indeterminate in two patients. Seventeen patients were treated based on results of short-term drug testing. During a mean follow-up period of 7.5 years, three patients died...
Oscillatory afterpotentials in dog ventricular muscle fibers.
Authors: MHiraoka, YOkamoto, TSano
Journal: Circulation Research (1981)

Ventricular muscle fibers from dog hearts were superfused with K+-free, high Ca2+ solutions after exposure to a K+-free, Ca2+-free superfusate. Following a series of driven action potentials, oscillatory after potentials (OAPs) were elicited in all 40 preparations at a resting potential of greater than -60 mV. The OAPs appeared not to represent either an electrotonic influence from Purkinje fibers or reentry. They showed a non-monotonic dependence on the basic cycle length, as well as on the number of preceding action potentials. When the OAPs attained threshold, there was extra-excitation (triggered activity) and, occasionally, sustained rhythmic activity. Single premature impulses increased the amplitude of the OAPs, but single or multiple stimuli applied during sustained rhythmic activity failed to stop it. The amplitude of the OAPs increased with higher external [Ca2+] and with lower external [Na+]. The OAPs and triggered activity were suppressed in the presence of Mn2+, verapamil, and 2.7 mM K+. Our ...
The influence of resting tension on immunoreactive atrial natriuretic peptide secretion by rat atria superfused in vitro.
Authors: R JSchiebinger, JLinden
Journal: Circulation Research (1986)

Atrial natriuretic peptide is a potent diuretic hormone secreted by the atria in response to volume expansion. We examined the effect of resting tension on atrial natriuretic peptide secretion by rat atria superfused in vitro. Left atria were hooked between an electrode and force transducer and superfused with medium 199. The atria were studied at a pacing frequency of 0 or 3 Hz. Atrial natriuretic peptide content of the superfusate was measured by radioimmunoassay. In nonpaced and paced atria, increasing resting tension three- to five-fold caused immunoreactive atrial natriuretic peptide secretion to increase by 35 +/- 5% (mean +/- SEM, n = 6, p less than 0.01) and 30 +/- 3% (n = 4, p less than 0.01), respectively. Lowering resting tension by 50% in nonpaced and paced atria lowered immunoreactive atrial natriuretic peptide secretion by 30 +/- 3% (n = 7, p less than 0.01) and 24 +/- 3% (n = 6, p less than 0.01), respectively. To exclude the possibility that release of norepinephrine or acetylcholine from ...
Heterosexual Transmission of AIDS
Authors: Richard B. Pearce
Journal: JAMA (1986)

<h3>To the Editor.—</h3> Because there are no data to indicate that "soldiers are more likely to lie (about homosexuality or IV drug use) than civilians," Redfield et al<sup>1</sup>conclude that they are telling the truth. Unfortunately, the mere absence of data to the contrary does not by itself make the opposite assertion true; if it did, science would be a much simpler thing. And yet the authors cite their observation (which is what it is) of acquired immunodeficiency syndrome (AIDS) among ten married servicemen, who claim contact with European prostitutes as their only risk factor, as evidence helping to substantiate the idea that human T-cell lymphotropic virus type III (HTLV-III) can be transmitted sexually from women to men. We are given the conclusion that "HTLV-III is a bidirectional sexually transmitted disease [<i>sic</i>]," which the authors find in "non-drug-using, strictly heterosexual men." While it is true that in Africa, the
Comment on: “Acral findings during the COVID-19 outbreak: Chilblain-like lesions should be preferred to acroischemic lesions”
Authors: Diego Fernandez-Nieto, Juan Jimenez-Cauhe, Ana Suarez-Valle, Oscar M. Moreno-Arrones, David Saceda-Corralo, Arantxa Arana-Raja, Daniel Ortega-Quijano
Journal: Journal of the American Academy of Dermatology (2020)
Angiotensin II-induced mitogenesis of spontaneously hypertensive rat-derived cultured smooth muscle cells is dependent on autocrine production of transforming growth factor-beta.
Authors: G AStouffer, G KOwens
Journal: Circulation Research (1992)

Angiotensin II (Ang II) has been implicated in the regulation of smooth muscle cell proliferation after vascular injury, but the molecular mechanisms of this effect remain obscure. The aims of the present study were 1) to determine if Ang II was mitogenic (in a defined serum-free medium) for aortic smooth muscle cells derived from spontaneously hypertensive rats, either alone or in combination with epidermal growth factor, basic fibroblast growth factor, or platelet-derived growth factor-BB; and 2) to determine if the Ang II effects were mediated by autocrine production of transforming growth factor-beta (TGF-beta). Results demonstrated that Ang II increased the proliferative response of smooth muscle cells to epidermal growth factor or platelet-derived growth factor-BB. Ang II alone and in combination with basic fibroblast growth factor induced a small delayed increase (48-72 hours after treatment) in DNA synthesis and [3H]thymidine labeling indexes without an increase in cell number. Ang II effects were...
The value of catheter mapping during sinus rhythm to localize site of origin of ventricular tachycardia.
Authors: D MCassidy, J AVassallo, A EBuxton, J UDoherty, F EMarchlinski, M EJosephson
Journal: Circulation (1984)

We assessed the value of endocardial catheter mapping in 52 patients in sinus rhythm and with 102 morphologically distinct ventricular tachycardias. The local bipolar electrograms from various regions of the left ventricle were assessed and quantitatively classified with respect to the characteristics of amplitude and duration. With the use of this assessment we found that electrograms from the site of origin were of significantly lower amplitude and longer duration; however, because such an overlap occurred with electrograms that were not from sites of origin, this does not serve as a useful clinical marker. Various types of electrograms, including normal, abnormal, fractionated, abnormal late, fractionated late, and longest, were evaluated with respect to sensitivity, specificity, and positive predictive value. None of these types possessed the ability to reliably localize the site of origin of ventricular tachycardia. We therefore conclude that endocardial catheter mapping during sinus rhythm is not us...