tbuckley/cabot-search huggingface dataset:
Antigen cross-presentation in young tumor-bearing hosts promotes CD8 <sup>+</sup> T cell terminal differentiation
Authors: Ardiana Moustaki, Jeremy Chase Crawford, Shanta Alli, Yiping Fan, Shannon Boi, Anthony E. Zamora, Natalie M. N. McDonald, Gang Wu, Joy Nakitandwe, Scott Newman, Scott Foy, Antonina Silkov, Paul G. Thomas, Alberto Pappo, Michael A. Dyer, Elizabeth Stewart, Sara Federico, Ben Youngblood
Journal: Science Immunology (2022)

The immune system undergoes a progressive functional remodeling with age. Understanding how age bias shapes antitumor immunity is essential in designing effective immunotherapies, especially for pediatric patients. Here, we explore antitumor CD8 + T cell responses generated in young (prepubescent) and adult (presenescent) mice. Using an MHCI-deficient tumor model, we observed that tumor-reactive CD8 + T cells expanded in young tumor-bearing (TB) mice acquired a terminally differentiated phenotype characterized by overexpression of inhibitory receptors and the transcription factor Tox1. Furthermore, tumor-infiltrating CD8 + T cells from young tumors yielded a poor cytokine response compared with CD8 + T cells infiltrating adult tumors. Young migratory dendritic cells (migDCs) from the draining lymph nodes (dLNs), and mononuclear phagocytic cells (MPCs) infiltrating young tumors, were more competent in capturing and cross-presenting tumor antigen, leading to enhanced priming of CD8 + T cells in dLNs and their subsequent terminal differentiation in the tumors. Single-cell transcriptional profiling of tumor-infiltrating MPCs demonstrated that young MPCs are polarized toward an inflammatory, effector phenotype. Consistent with our observations in young versus adult TB mice, analysis of immune infiltrates from pediatric solid tumors showed a correlation between tumor-infiltrating CD8 + T cells with an exhaustion phenotype and the frequency of PD-L1–expressing monocytes/macrophages. Collectively, these data indicate that a young tissue microenvironment contributes to the generation of an immune response skewed toward a less pliable terminal effector state, thus narrowing the window for immunotherapeutic interventions.
Lyophilization provides long-term stability for a lipid nanoparticle-formulated, nucleoside-modified mRNA vaccine
Authors: Hiromi Muramatsu, Kieu Lam, Csaba Bajusz, Dorottya Laczkó, Katalin Karikó, Petra Schreiner, Alan Martin, Peter Lutwyche, James Heyes, Norbert Pardi
Journal: Molecular Therapy (2022)

Lipid nanoparticle (LNP)-formulated nucleoside-modified mRNA vaccines have proven to be very successful in the fight against the coronavirus disease 2019 (COVID-19) pandemic. They are effective, safe, and can be produced in large quantities. However, the long-term storage of mRNA-LNP vaccines without freezing is still a challenge. Here, we demonstrate that nucleoside-modified mRNA-LNPs can be lyophilized, and the physicochemical properties of the lyophilized material do not significantly change for 12 weeks after storage at room temperature and for at least 24 weeks after storage at 4°C. Importantly, we show in comparative mouse studies that lyophilized firefly luciferase-encoding mRNA-LNPs maintain their high expression, and no decrease in the immunogenicity of a lyophilized influenza virus hemagglutinin-encoding mRNA-LNP vaccine was observed after 12 weeks of storage at room temperature or for at least 24 weeks after storage at 4°C. Our studies offer a potential solution to overcome the long-term storage-related limitations of nucleoside-modified mRNA-LNP vaccines.
Mepolizumab Induced Loss of Smell Improvement in Patients With Chronic Rhinosinusitis With Nasal Polyps From the SYNAPSE Study
Authors: Joaquim Mullol, Valerie Lund, Martin Wagenmann, Joseph Han, Ana Sousa, Steven Smith, Bhabita Mayer, Steven Yancey, Robert Chan, Wytske Fokkens
Journal: Journal of Allergy and Clinical Immunology (2022)
The Impact Of Baseline Exhaled Nitric Oxide Levels And Blood Eosinophil Count On Clinical Outcomes In REALITI-A
Authors: David Bernstein, Mark Liu, Florence Schleich, Jason Lee, Girolamo Pelaia, Carlos Almonacid Sanchez, Rekha Chaudhuri, Rafael Alfonso Cristancho, Rupert Jakes, Robert Price, Aoife Maxwell, Peter Howarth
Journal: Journal of Allergy and Clinical Immunology (2022)
Society Proceedings
Journal: JAMA (1931)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Fabrication of Tβ4-Exosome-releasing artificial stem cells for myocardial infarction therapy by improving coronary collateralization
Authors: Peier Chen, Xiaodong Ning, Weirun Li, Yuxuan Pan, Ling Wang, Hekai Li, Xianglin Fan, Jiexin Zhang, Tiantian Luo, Yaobin Wu, Caiwen Ou, Minsheng Chen
Journal: Bioactive Materials (2022)

Currently, stem cell transplantations in cardiac repair are limited owing to disadvantages, such as immunological rejection and poor cell viability. Although direct injection of exosomes can have a curative effect similar to that of stem cell transplantation, high clearance hinders its application in clinical practice. Previous reports suggested that induction of coronary collateralization can be a desired method of adjunctive therapy for someone who had missed the optimal operation time to attenuate myocardial ischemia. In this study, to mimic the paracrine and biological activity of stem cells, we developed artificial stem cells that can continuously release Tβ4-exosomes (Tβ4-ASCs) by encapsulating specific exosomes within microspheres using microfluidics technology. The results show that Tβ4-ASCs can greatly promote coronary collateralization in the periphery of the myocardial infarcted area, and its therapeutic effect is superior to that of directly injecting the exosomes. In addition, to better understand how it works, we demonstrated that the Tβ4-ASC-derived exosomes can enhance the angiogenic capacity of coronary endothelial cells (CAECs) via the miR-17-5p/PHD3/Hif-1α pathway. In brief, as artificial stem cells, Tβ4-ASCs can constantly release functional exosomes and stimulate the formation of collateral circulation after myocardial infarction, providing a feasible and alternative method for clinical revascularization.
Premotor activity in the claustrum
Authors: Alison D. Do, Jesse Jackson
Journal: Neuron (2022)

In this issue of Neuron, Chevée et al. (2022) performed extracellular electrophysiological recordings from claustrum neurons during a sensory selection task. They found that neural activity in the claustrum reflected future motor output rather than sensory inputs and that chemogenetic suppression of claustrum activity reduced motor impulsivity in this task.
Blood cultures following rectal examination
Authors: D. Tandberg
Journal: JAMA (1978)

CERTAIN types of manipulative procedures may be followed by transient bacteremia.<sup>1</sup>These procedures include dental work, tonsillectomy, tracheal intubation, bronchoscopy, esophageal dilation, upper gastrointestinal (GI) tract endoscopy, liver biopsy, child-birth, cardiac catheterization, angiography, and examinations or surgery involving the rectum and prostate. The frequency of bacteremia following lower GI tract manipulation has varied in different reports, perhaps depending on the type of procedure and the technique used, the timing of blood samples, the culture techniques, and the investigator's interpretation regarding which of the recovered organisms were from the blood and which were contaminants. Sigmoidos-copy-associated bacteremia rates have varied from 0% to 9.5%,<sup>1,2</sup>colonoscopy rates from 0% to 5.6%,<sup>1,3</sup>and the rate associated with barium enema has been reported to be from 11.4% to 40%.<sup>1,4</sup>Prostatic manipulation can result in frequent bacteremia under certain circumstances, as illustrated by a reported 67% bacteremia rate following massage of apparently
Conductive Polymer Enabled Biostable Liquid Metal Electrodes for Bioelectronic Applications
Authors: Taehwan Lim, Minju Kim, Amir Akbarian, Jungkyu Kim, Patrick A. Tresco, Huanan Zhang
Journal: Advanced Healthcare Materials (2022)

Gallium (Ga)-based liquid metal materials have emerged as a promising material platform for soft bioelectronics. Unfortunately, Ga has limited biostability and electrochemical performance under physiological conditions, which can hinder the implementation of its use in bioelectronic devices. Here, an effective conductive polymer deposition strategy on the liquid metal surface to improve the biostability and electrochemical performance of Ga-based liquid metals for use under physiological conditions is demonstrated. The conductive polymer [poly(3,4-ethylene dioxythiophene):tetrafluoroborate]-modified liquid metal surface significantly outperforms the liquid metal.based electrode in mechanical, biological, and electrochemical studies. In vivo action potential recordings in behaving nonhuman primate and invertebrate models demonstrate the feasibility of using liquid metal electrodes for high-performance neural recording applications. This is the first demonstration of single-unit neural recording using Ga-based liquid metal bioelectronic devices to date. The results determine that the electrochemical deposition of conductive polymer over liquid metal can improve the material properties of liquid metal electrodes for use under physiological conditions and open numerous design opportunities for next-generation liquid metal-based bioelectronics.
Full Circle
Authors: Reem A. Abu-Libdeh
Journal: JAMA (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Saving children from man-made acute malnutrition in Tigray, Ethiopia: a call to action
Authors: Afework Mulugeta, Mulugeta Gebregziabher
Journal: The Lancet Global Health (2022)

Armed conflict has been raging in Tigray, a region in northern Ethiopia, since Nov 4, 2020. Some of the defining characteristics of this armed conflict are a total blockade of humanitarian aid, wanton destruction of health facilities, and the displacement of millions of people.1–5 These weaponised actions have exposed children in Tigray to preventable severe and moderate acute malnutrition. Thousands of civilians are facing man-made starvation and millions more acute food insecurity.6,7
Searching for a better mousetrap for ablation of Barrett’s esophagus
Authors: John A. Dumot
Journal: Endoscopy (2022)

I am pleased to share some insights on the article by Gotink et al. in this issue of Endoscopy: “Nonthermal resection device for ablation of Barrett’s esophagus: a feasibility and safety study” [1]. The authors are experts in both endoscopic therapy and clinical trials, making this an ideal team to evaluate the novel nonthermal resection device (EndoRotor Mucosal Resection device; Interscope Inc., Northbridge, Massachusetts, USA) for patients with Barrett’s esophagus. Well-meaning endoscopists must consider the risks of new technologies over the standard-of-care when they act as early adopters. Thus, I applaud the editors for choosing this well-performed clinical trial. We are cognizant of bias against reporting ineffective treatments and poor outcomes, especially with a trend toward industry funding, and publishing positive outcomes [2].
The Prevalence of Dysphagia and Food Impactions is High in Atopic Patients Without a Known History of Eosinophilic Esophagitis (EoE)
Authors: Ryan Eid, Emily Noonan, Jaimin Patel, Barrett Barnes, Bryan Sauer, Jeffrey Wilson, Amani Al-Hazaymeh, Emily McGowan
Journal: Journal of Allergy and Clinical Immunology (2022)
Errors in Abstract and Results Section
Journal: JAMA Dermatology (2021)

Adolescents With Moderate-to-Severe Atopic Dermatitis: The JADE TEEN Randomized Clinical Trial," 1 the phrasing of sentences in the Safety section of the Results was corrected, "PP-NRS total score" was changed to "PP-NRS total score, mean (SD)" in Table 1, and 6 superscript letters in Figure 3 were changed from " c " or " d " to " a ".This article was corrected on October 6, 2021.
Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments
Authors: Shogo Kumagai, Shohei Koyama, Kota Itahashi, Tokiyoshi Tanegashima, Yi-Tzu Lin, Yosuke Togashi, Takahiro Kamada, Takuma Irie, Genki Okumura, Hidetoshi Kono, Daisuke Ito, Rika Fujii, Sho Watanabe, Atsuo Sai, Shota Fukuoka, Eri Sugiyama, Go Watanabe, Takuya Owari, Hitomi Nishinakamura, Daisuke Sugiyama, Yuka Maeda, Akihito Kawazoe, Hiroki Yukami, Keigo Chida, Yuuki Ohara, Tatsuya Yoshida, Yuki Shinno, Yuki Takeyasu, Masayuki Shirasawa, Kenta Nakama, Keiju Aokage, Jun Suzuki, Genichiro Ishii, Takeshi Kuwata, Naoya Sakamoto, Masahito Kawazu, Toshihide Ueno, Taisuke Mori, Naoya Yamazaki, Masahiro Tsuboi, Yasushi Yatabe, Takahiro Kinoshita, Toshihiko Doi, Kohei Shitara, Hiroyuki Mano, Hiroyoshi Nishikawa
Journal: Cancer Cell (2022)
February 1980, vol 64, No. 2
Journal: JNCI Journal of the National Cancer Institute (1980)

February 1980, vol 64, No. 2 Get access JNCI: Journal of the National Cancer Institute, Volume 64, Issue 6, June 1980, Page 1571, https://doi.org/10.1093/jnci/64.6.1571 Published: 01 June 1980
Epithelial barrier hypothesis: Effect of the external exposome on the microbiome and epithelial barriers in allergic disease
Authors: Zeynep Celebi Sozener, Betul Ozdel Ozturk, Pamir Cerci, Murat Turk, Begum Gorgulu Akin, Mubeccel Akdis, Seda Altiner, Umus Ozbey, Ismail Ogulur, Yasutaka Mitamura, Insu Yilmaz, Kari Nadeau, Cevdet Ozdemir, Dilsad Mungan, Cezmi A. Akdis
Journal: Allergy (2022)

Environmental exposure plays a major role in the development of allergic diseases. The exposome can be classified into internal (e.g., aging, hormones, and metabolic processes), specific external (e.g., chemical pollutants or lifestyle factors), and general external (e.g., broader socioeconomic and psychological contexts) domains, all of which are interrelated. All the factors we are exposed to, from the moment of conception to death, are part of the external exposome. Several hundreds of thousands of new chemicals have been introduced in modern life without our having a full understanding of their toxic health effects and ways to mitigate these effects. Climate change, air pollution, microplastics, tobacco smoke, changes and loss of biodiversity, alterations in dietary habits, and the microbiome due to modernization, urbanization, and globalization constitute our surrounding environment and external exposome. Some of these factors disrupt the epithelial barriers of the skin and mucosal surfaces, and these disruptions have been linked in the last few decades to the increasing prevalence and severity of allergic and inflammatory diseases such as atopic dermatitis, food allergy, allergic rhinitis, chronic rhinosinusitis, eosinophilic esophagitis, and asthma. The epithelial barrier hypothesis provides a mechanistic explanation of how these factors can explain the rapid increase in allergic and autoimmune diseases. In this review, we discuss factors affecting the planet's health in the context of the 'epithelial barrier hypothesis,' including climate change, pollution, changes and loss of biodiversity, and emphasize the changes in the external exposome in the last few decades and their effects on allergic diseases. In addition, the roles of increased dietary fatty acid consumption and environmental substances (detergents, airborne pollen, ozone, microplastics, nanoparticles, and tobacco) affecting epithelial barriers are discussed. Considering the emerging data from recent studies, we suggest stringent governmental regulations, global policy adjustments, patient education, and the establishment of individualized control measures to mitigate environmental threats and decrease allergic disease.
Can medical product development be better aligned with global needs?
Authors: Vasee Moorthy, Gavin Yamey
Journal: BMJ (2022)

Go to any pharmacy and you'll see a dizzying array of shampoos and facial moisturizers with new kinds emerging all the time.There is no medical need for more ways to wash your hair or moisturize your skin, but the huge financial returns guarantee continual investment by companies into rebranding.By contrast, there are many disabling or fatal diseases for which we desperately need new medical products (such as medicines, vaccines, and diagnostics)-yet few, or none, are available and the product development pipeline is bare. 1 2 Even when these medical products do become available, often they reach high income-countries first, with a time lag of up to several decades before they become available in lower income settings.How has such inequity arisen and how can it be addressed?How drug companies determine whether to engage in product development OPINION
Errors in Abstract, Table 3, and Table 4
Journal: JAMA Network Open (2022)

Joyce M. Lee, MD, MPH; Andrea Rusnak, MS; Ashley Garrity, MPH; Emily Hirschfeld, BA; Inas H. Thomas, MD; Michelle Wichorek, PhD; Jung Eun Lee, MS; Nicole A. Rioles, MA; Osagie Ebekozien, MD, MPH, CPHQ; Sarah D. Corathers, MD
<i>In Vivo</i> Thrombosis Imaging in Patients Recovering from COVID-19 and Pulmonary Embolism
Authors: Rong Bing, Jack P. M. Andrews, Michelle C. Williams, Edwin J. R. van Beek, Christophe Lucatelli, Gillian MacNaught, Tim Clark, Norman Koglin, Andrew W. Stephens, Mark G. MacAskill, Adriana A. S. Tavares, Kevin Dhaliwal, David A. Dorward, Christopher D. Lucas, Marc R. Dweck, David E. Newby
Journal: American Journal of Respiratory and Critical Care Medicine (2021)

Protracted macrovascular and microvascular thrombosis of the systemic and pulmonary circulation is a feature of COVID-19 that persists despite systemic therapeutic anticoagulation.
Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing
Authors: Yuzhen Wang, Xingyu Yuan, Bin Yao, Shuoji Zhu, Ping Zhu, Sha Huang
Journal: Bioactive Materials (2022)

Extrusion-based bioprinting (EBB) holds potential for regenerative medicine. However, the widely-used bioinks of EBB exhibit some limitations for skin regeneration, such as unsatisfactory bio-physical (i.e., mechanical, structural, biodegradable) properties and compromised cellular compatibilities, and the EBB-based bioinks with therapeutic effects targeting cutaneous wounds still remain largely undiscussed. In this review, the printability considerations for skin bioprinting were discussed. Then, current strategies for improving the physical properties of bioinks and for reinforcing bioinks in EBB approaches were introduced, respectively. Notably, we highlighted the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vascularization and the regeneration of skin appendages (i.e., sweat glands and hair follicles) and discussed the challenges and future perspectives. This review aims to provide an overall view of the applications, challenges and promising solutions about the EBB-based bioinks for cutaneous wound healing and skin regeneration.
Use of <i>Vibrio cholerae</i> Vaccine in an Outbreak in Guinea
Authors: Francisco J. Luquero, Lise Grout, Iza Ciglenecki, Keita Sakoba, Bala Traore, Melat Heile, Alpha Amadou Diallo, Christian Itama, Anne-Laure Page, Marie-Laure Quilici, Martin A. Mengel, Jose Maria Eiros, Micaela Serafini, Dominique Legros, Rebecca F. Grais
Journal: New England Journal of Medicine (2014)

The use of vaccines to prevent and control cholera is currently under debate. Shanchol is one of the two oral cholera vaccines prequalified by the World Health Organization; however, its effectiveness under field conditions and the protection it confers in the first months after administration remain unknown. The main objective of this study was to estimate the short-term effectiveness of two doses of Shanchol used as a part of the integrated response to a cholera outbreak in Africa.We conducted a matched case-control study in Guinea between May 20 and October 19, 2012. Suspected cholera cases were confirmed by means of a rapid test, and controls were selected among neighbors of the same age and sex as the case patients. The odds of vaccination were compared between case patients and controls in bivariate and adjusted conditional logistic-regression models. Vaccine effectiveness was calculated as (1-odds ratio)×100.Between June 8 and October 19, 2012, we enrolled 40 case patients and 160 controls in the study for the primary analysis. After adjustment for potentially confounding variables, vaccination with two complete doses was associated with significant protection against cholera (effectiveness, 86.6%; 95% confidence interval, 56.7 to 95.8; P=0.001).In this study, Shanchol was effective when used in response to a cholera outbreak in Guinea. This study provides evidence supporting the addition of vaccination as part of the response to an outbreak. It also supports the ongoing efforts to establish a cholera vaccine stockpile for emergency use, which would enhance outbreak prevention and control strategies. (Funded by Médecins sans Frontières.).
Validation of Remote AD Severity Assessment with the Eczema Area and Severity Index (EASI) Using Photos and Videos: Preliminary Reliability and Validity
Authors: Emily Croce, Paul Rathouz, Fabiana Castro Porto Silva Lopes, Maria Leszczynska, Lucia Diaz, Moise Levy, Jennifer Ruth, Pooja Varshney, Elizabeth Matsui
Journal: Journal of Allergy and Clinical Immunology (2022)