tbuckley/cabot-search huggingface dataset:
Bilateral giant cell arteritis with skin necrosis following SARS‐CoV‐2 vaccination
Authors: T. Gambichler, C. Krogias, I. Tischoff, A. Tannapfel, R. Gold, L. Susok
Journal: British Journal of Dermatology (2021)

Journal Article Bilateral giant cell arteritis with skin necrosis following SARS‐CoV‐2 vaccination Get access T. Gambichler, T. Gambichler Skin Cancer Center Department of Dermatology Ruhr‐University Bochum BochumGermany https://orcid.org/0000-0001-7862-3695 Search for other works by this author on: Oxford Academic Google Scholar C. Krogias, C. Krogias Department of Neurology Ruhr‐University Bochum BochumGermany Search for other works by this author on: Oxford Academic Google Scholar I. Tischoff, I. Tischoff Institute of Pathology Ruhr‐University Bochum Bochum Germany Search for other works by this author on: Oxford Academic Google Scholar A. Tannapfel, A. Tannapfel Institute of Pathology Ruhr‐University Bochum Bochum Germany Search for other works by this author on: Oxford Academic Google Scholar R. Gold, R. Gold Department of Neurology Ruhr‐University Bochum BochumGermany Search for other works by this author on: Oxford Academic Google Scholar L. Susok L. Susok Skin Cancer Center Department of Dermatology Ruhr‐University Bochum BochumGermany Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 186, Issue 2, 1 February 2022, Page e83, https://doi.org/10.1111/bjd.20824 Published: 01 February 2022
TCT-492 External Validation of the SYNTAX Score II 2020 in the CREDO-Kyoto PCI/CABG Registry
Authors: Hironori Hara, Hiroki Shiomi, David van Klaveren, David Kent, Ewout W. Steyerberg, Scot Garg, Yoshinobu Onuma, Takeshi Kimura, Patrick Serruys
Journal: Journal of the American College of Cardiology (2021)
Crosstalk between autophagy inhibitors and endosome-related secretory pathways: a challenge for autophagy-based treatment of solid cancers
Authors: Martina Raudenska, Jan Balvan, Michal Masarik
Journal: Molecular Cancer (2021)

Abstract Autophagy is best known for its role in organelle and protein turnover, cell quality control, and metabolism. The autophagic machinery has, however, also adapted to enable protein trafficking and unconventional secretory pathways so that organelles (such as autophagosomes and multivesicular bodies) delivering cargo to lysosomes for degradation can change their mission from fusion with lysosomes to fusion with the plasma membrane, followed by secretion of the cargo from the cell. Some factors with key signalling functions do not enter the conventional secretory pathway but can be secreted in an autophagy-mediated manner. Positive clinical results of some autophagy inhibitors are encouraging. Nevertheless, it is becoming clear that autophagy inhibition, even within the same cancer type, can affect cancer progression differently. Even next-generation inhibitors of autophagy can have significant non-specific effects, such as impacts on endosome-related secretory pathways and secretion of extracellular vesicles (EVs). Many studies suggest that cancer cells release higher amounts of EVs compared to non-malignant cells, which makes the effect of autophagy inhibitors on EVs secretion highly important and attractive for anticancer therapy. In this review article, we discuss how different inhibitors of autophagy may influence the secretion of EVs and summarize the non-specific effects of autophagy inhibitors with a focus on endosome-related secretory pathways. Modulation of autophagy significantly impacts not only the quantity of EVs but also their content, which can have a deep impact on the resulting pro-tumourigenic or anticancer effect of autophagy inhibitors used in the antineoplastic treatment of solid cancers.
Abstract 14754: Detection of Significant Stenosis in Left Anterior Descending Branch on Conventional Coronary Angiogram by Virtual "Myocardial Perfusion" Bolus Tracking CT Images in Coronary 320 Slice CT Angiography
Authors: Hiroyuki Takaoka, Nobusada Funabashi, Koya Ozawa, Yoshihide Fujimoto, Yoshio Kobayashi
Journal: Circulation (2013)

Purpose: Diagnostic accuracy of coronary CT angiography is impaired by severe coronary calcification and surrogate measurements without additional radiation exposure are needed. We used bolus track...
Birth Outcomes in Women Who Have Taken Hydroxycholoroquine During Pregnancy: A Prospective Cohort Study
Authors: Christina D. Chambers, Diana L. Johnson, Ronghui Xu, Yunjun Luo, Robert Felix, Minh Fine, Chloe Lessard, Margaret P. Adam, Stephen R. Braddock, Luther K. Robinson, Leah Burke, Kenneth Lyons Jones
Journal: Arthritis & Rheumatology (2021)

Findings from previous small studies have been reassuring regarding the safety of treatment with hydroxychloroquine (HCQ) during pregnancy. In one recent study, it was demonstrated that the frequency of major birth defects was increased in women who had received HCQ at a dose of ≥400 mg/day during pregnancy. This study was undertaken to examine pregnancy outcomes among women following the use of HCQ.
Direct oral penicillin challenge for penicillin allergy delabeling as a health services intervention: A multicenter cohort study
Authors: Jason A. Trubiano, Sara Vogrin, Ana Copaescu, Mohamed Nasra, Abby Douglas, Natasha E. Holmes, Kyra Y. L. Chua
Journal: Allergy (2021)

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
TCT-446 NobleStitch EL Use for PFO Closure: A Multicenter Experience
Authors: Lars Witte, Mick Renkens, Aleksandra Gąsecka, Gregor Leibundgut, Daniel Weilenmann, Robbert De Winter, Pieter Stella, Michiel Voskuil
Journal: Journal of the American College of Cardiology (2021)
Biomaterial Wettability Affects Fibrin Clot Structure and Fibrinolysis (Adv. Healthcare Mater. 20/2021)
Authors: Alexander M. Ruhoff, Jun Ki Hong, Lingzi Gao, Jasneil Singh, Clara Tran, Grace Mackie, Anna Waterhouse
Journal: Advanced Healthcare Materials (2021)

Biomaterial Fibrin Clot Structures Stylized fibrin clots are presented in article number 2100988 by Anna Waterhouse and co-workers. Less dense clots on hydrophobic (right) compared to hydrophilic (left) polystyrene are more easily lysed, demonstrating potential for targeted fibrinolytic treatment and improved hemocompatible device design.
Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model
Authors: Shun Itai, Hiroaki Onoe
Journal: Advanced Healthcare Materials (2021)

Since the biochemical reaction of blood vessels plays an essential role in immune response or various diseases, in vitro vascular models have high demand from medical fields. However, vascular models often tend to be difficult to mimic the biomedical reaction faithfully because of the lack of implementation of the tissue deformation. Here, an inflammatory mediator-induced deformation reaction of a blood vessel on a flexibly deformable collagen hydrogel tube device is reproduced. A self-standing collagen tube enables the tissue to deform flexibly in biochemical reaction and achieves contraction both at tissue and cell level. The contraction of tissue relieves the stress between cells under reaction to maintain cellular junctions even tight junctions are broken. Also, the drug perfusion test with antihistamine chemical is easily performed due to the connector part and properly inhibits the inflammatory reaction. Moreover, the traction force on endothelial cells is analyzed as about 0.9 µN on two types of scaffolds with different stiffness. It is believed that the potential of the flexible tissue model to reproduce biochemical reactions can contribute to the fabrication of vascular tissue models mimicking in vivo in high similarity as a platform for biomedical researches and pharmacokinetic testing.
Novel Noninvasive Approaches to the Treatment of Obesity: From Pharmacotherapy to Gene Therapy
Authors: Angeliki M Angelidi, Matthew J Belanger, Alexander Kokkinos, Chrysi C Koliaki, Christos S Mantzoros
Journal: Endocrine Reviews (2021)

Recent insights into the pathophysiologic underlying mechanisms of obesity have led to the discovery of several promising drug targets and novel therapeutic strategies to address the global obesity epidemic and its comorbidities. Current pharmacologic options for obesity management are largely limited in number and of modest efficacy/safety profile. Therefore, the need for safe and more efficacious new agents is urgent. Drugs which are currently under investigation modulate targets across a broad range of systems and tissues, including the central nervous system, gastrointestinal hormones, adipose tissue, kidney, liver, and skeletal muscle. Beyond pharmacotherapeutics, other potential antiobesity strategies are being explored, including novel drug delivery systems, vaccines, modulation of the gut microbiome, and gene therapy. The present review summarizes the pathophysiology of energy homeostasis, and highlights pathways being explored in the effort to develop novel antiobesity medications and interventions but does not cover devices and bariatric methods. Emerging pharmacologic agents and alternative approaches targeting these pathways and relevant research in both animals and humans are presented in detail. Special emphasis is given to treatment options at the end of the development pipeline and closer to the clinic, i.e., compounds that have a higher chance to be added to our therapeutic armamentarium in the near future. Ultimately, advancements in our understanding of the pathophysiology and interindividual variation of obesity may lead to multimodal and personalized approaches to obesity treatment that will result in safe, effective and sustainable weight loss until the root causes of the problem are identified and addressed.
Microbial contribution to the caloric restriction-triggered regulation of the intestinal levels of glutathione transferases, taurine, and bile acid
Authors: András Gregor, Marc Pignitter, Slave Trajanoski, Sandra Auernigg-Haselmaier, Veronika Somoza, Jürgen König, Kalina Duszka
Journal: Gut Microbes (2021)

Recently we showed that caloric restriction (CR) triggers an increase in the levels of free taurine, taurine-conjugated bile acids (BA), and other taurine conjugates in intestinal mucosa while decreasing glutathione (GSH) levels in wild-type male mice. In the current project, we decided to investigate whether the microbiota is involved in the response to CR by depleting gut bacteria. The antibiotics treatment diminished CR-specific increase in the levels of free taurine and its conjugates as well as upregulated expression and activity of GSH transferases (GST) in the intestinal mucosa. Further, it diminished a CR-related increase in BAs levels in the liver, plasma, and intestinal mucosa. Transplant of microbiota from CR mice to ad libitum fed mice triggered CR-like changes in MGST1 expression, levels of taurine and taurine conjugates in the mucosa of the ileum. We show for the first time, that microbiota contributes to the intestinal response to CR-triggered changes in BA, taurine, and GST levels.
World leaders must act now to prevent next pandemic, warns Global Preparedness Monitoring Board
Authors: Ingrid Torjesen
Journal: BMJ (2021)

Time is running out for world leaders to act to end the current covid-19 pandemic and to introduce measures to prevent future pandemics, the Global Preparedness Monitoring Board (GPMB) has said.In its 2021 report the board issued a stark warning that the "window of opportunity to make meaningful change" was closing quickly as the world's attention turned to other issues.
TCT-357 Diagnostic Accuracy of Dynamic Stress Myocardial CT Perfusion as Compared With Invasive Coronary Physiology Metrics in Patients With Suspected In-Stent Restenosis or CAD Progression: Preliminary Results of ADVANTAGE 2 Study
Authors: Saima Mushtaq, Edoardo Conte, Daniela Trabattoni, Luca Grancini, Sarah Troiano, Giovanni Monizzi, Carlos Collet, Jeroen Sonck, Daniele Andreini
Journal: Journal of the American College of Cardiology (2021)
Predicting the recurrence of chronic rhinosinusitis with nasal polyps using nasal microbiota
Authors: Yan Zhao, Junru Chen, Yun Hao, Boqian Wang, Yue Wang, Qinghua Liu, Jinming Zhao, Ying Li, Ping Wang, Xiangdong Wang, Peng Zhang, Luo Zhang
Journal: Allergy (2021)

Recent studies have revealed that the nasal microbiota in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) is profoundly altered and is correlated with systemic inflammation. However, little is known regarding whether the microbiota can be utilized to predict nasal polyp recurrence. This study is aimed to determine whether altered nasal microbiota constituents could be used as biomarkers to predict CRSwNP recurrence.Nasal microbiota constituents were quantified and characterized using bacterial 16S ribosomal RNA gene sequencing. Selected features for least absolute shrinkage and selection operator regression-based predictors were the nasal microbiota community composition and CRSwNP patient clinical characteristics. The primary outcome was recurrence, which was determined post-admission.By distinguishing recurrence-associated nasal microbiota taxa and exploiting the distinct nasal microbiota abundance between patients with recurrent and non-recurrent CRSwNP, we developed a predictive classifier for the diagnosis of nasal polyps' recurrence with 91.4% accuracy.Key taxonomical features of the nasal microbiome could predict recurrence in CRSwNP patients. The nasal microbiome is an understudied source of clinical variation in CRSwNP and represents a novel therapeutic target for future prevention and treatment.
Strategies for Promotion of a Healthy Lifestyle in Clinical Settings: Pillars of Ideal Cardiovascular Health: A Science Advisory From the American Heart Association
Authors: Penny M. Kris-Etherton, Kristina S. Petersen, Jean-Pierre Després, Cheryl A.M. Anderson, Prakash Deedwania, Karen L. Furie, Scott Lear, Alice H. Lichtenstein, Felipe Lobelo, Pamela B. Morris, Frank M. Sacks, Jun Ma
Journal: Circulation (2021)

Engagement in healthy lifestyle behaviors is suboptimal. The vast majority of the US population does not meet current recommendations. A healthy lifestyle is defined by consuming a healthy dietary pattern, engaging in regular physical activity, avoiding exposure to tobacco products, habitually attaining adequate amounts of sleep, and managing stress levels. For all these health behaviors there are well-established guidelines; however, promotion in clinical settings can be challenging. It is critical to overcome these challenges because greater promotion of heathy lifestyle practices in clinical settings effectively motivates and initiates patient behavior change. The 5A Model (assess, advise, agree, assist, and arrange) was developed to provide a framework for clinical counseling with requisite attention to the demands of clinical settings. In this science advisory, we present strategies, based on the 5A Model, that clinicians and other health care professionals can use for efficient lifestyle-related behavior change counseling in patients at all levels of cardiovascular disease risk at every visit. In addition, we discuss the underlying role of psychological health and well-being in lifestyle-related behavior change counseling, and how clinicians can leverage health technologies when providing brief patient-centered counseling. Greater attention to healthy lifestyle behaviors during routine clinician visits will contribute to promoting cardiovascular health.
A unique role of p53 haploinsufficiency or loss in the development of acute myeloid leukemia with FLT3-ITD mutation
Authors: Min Yang, Zengkai Pan, Kezhi Huang, Guntram Büsche, Hongyun Liu, Gudrun Göhring, Regina Rumpel, Oliver Dittrich-Breiholz, Steven Talbot, Michaela Scherr, Anuhar Chaturvedi, Matthias Eder, Julia Skokowa, Jianfeng Zhou, Karl Welte, Nils von Neuhoff, Ligen Liu, Arnold Ganser, Zhixiong Li
Journal: Leukemia (2021)

Abstract With an incidence of ~50%, the absence or reduced protein level of p53 is much more common than TP53 mutations in acute myeloid leukemia (AML). AML with FLT3 -ITD (internal tandem duplication) mutations has an unfavorable prognosis and is highly associated with wt-p53 dysfunction. While TP53 mutation in the presence of FLT3-ITD does not induce AML in mice, it is not clear whether p53 haploinsufficiency or loss cooperates with FLT3 -ITD in the induction of AML. Here, we generated FLT3 -ITD knock-in; p53 knockout (heterozygous and homozygous) double-transgenic mice and found that both alterations strongly cooperated in the induction of cytogenetically normal AML without increasing the self-renewal potential. At the molecular level, we found the strong upregulation of Htra3 and the downregulation of Lin28a , leading to enhanced proliferation and the inhibition of apoptosis and differentiation. The co-occurrence of Htra3 overexpression and Lin28a knockdown, in the presence of FLT3-ITD, induced AML with similar morphology as leukemic cells from double-transgenic mice. These leukemic cells were highly sensitive to the proteasome inhibitor carfilzomib. Carfilzomib strongly enhanced the activity of targeting AXL (upstream of FLT3 ) against murine and human leukemic cells. Our results unravel a unique role of p53 haploinsufficiency or loss in the development of FLT3 -ITD + AML.
TCT-10 Ten-Year Follow-Up Outcomes in Diabetic Patients With ST-Segment Elevation Myocardial Infarction: Insights From the Examination-Extend Trial
Authors: Francesco Spione, Victor Arevalos, Rami Gabani, Victor Alfonso Jimenez Diaz, Marcelo Jimenez, Pilar Jimenez Quevedo, Roberto Diletti, Vicente Mainar Tello, Gianluca Campo, Antonio Silvestro, Jaume Maristany, Xacobe Flores-Ríos, Patrick Serruys, Salvatore Brugaletta
Journal: Journal of the American College of Cardiology (2021)
Applications of CRISPR technology to lung cancer research
Authors: Anabel Sorolla, Eva Parisi, Maria Alba Sorolla, Marta Marqués, José M. Porcel
Journal: European Respiratory Journal (2021)

<b>CRISPR technology has revolutionised lung cancer research. It can be used to model lung cancer in animals, to find new players in cancer initiation and progression, to diagnose earlier, and even to design novel gene therapies.</b>https://bit.ly/3GPjT6o
TCT-106 Transcatheter Edge-To-Edge Mitral Valve Repair in Non–COAPT-Like Patients: Incidence and Predictors of 2-Year Good Outcome
Authors: Andrea Scotti, Andrea Munafò, Marianna Adamo, Maurizio Taramasso, Paolo Denti, Antonio Sisinni, Nicola Buzzatti, Stefano Stella, Francesco Ancona, Gregorio Zaccone, Dario Cani, Matteo Montorfano, Alessandro Castiglioni, Michele De Bonis, Ottavio Alfieri, Azeem Latib, Antonio Colombo, Eustachio Agricola, Francesco Maisano, Marco Metra, Alberto Margonato, Cosmo Godino
Journal: Journal of the American College of Cardiology (2021)
TCT-418 Thick Biodegradable-Polymer Biolimus A9–Eluting Stent Versus Thin Durable-Polymer Zotarolimus-Eluting Stent in Multivessel Percutaneous Coronary Intervention
Authors: Yun-Kyeong Cho, Ung Kim, Joon-Hyung Doh, Bon-Kwon Koo, Eun-Seok Shin, Cheol Hyun Lee, In-Cheol Kim, Seung-Ho Hur
Journal: Journal of the American College of Cardiology (2021)
Neuroendocrine Cell Hyperplasia of Infancy (NEHI)/Hiperplasia de Celulas Neuroendocrina de la Infancia (NEHI)
Authors: David Spielberg, David Moreno-McNeil, Marianna Sockrider
Journal: American Journal of Respiratory and Critical Care Medicine (2021)

"Neuroendocrine Cell Hyperplasia of Infancy (NEHI)/Hiperplasia de Celulas Neuroendocrina de la Infancia (NEHI)." American Journal of Respiratory and Critical Care Medicine, 204(9), pp. P15–P16
“ADPKD-omics”: determinants of cyclic AMP levels in renal epithelial cells
Authors: Yash R. Mehta, Spencer A. Lewis, Kirby T. Leo, Lihe Chen, Euijung Park, Viswanathan Raghuram, Chung-Lin Chou, Chin-Rang Yang, Hiroaki Kikuchi, Syed Khundmiri, Brian G. Poll, Mark A. Knepper
Journal: Kidney International (2021)
RNAi-mediated silencing in hard-to-transfect cell types - From single gene analysis to screening applications
Authors: Markus Zumbansen, Dietmar Lenz, Ludger Altrogge, Herbert Muller-Hartmann
Journal: Cancer Research (2007)

1191  RNAi-mediated gene knockdown is a powerful tool that is very successfully applied to identify gene function and elucidate biological pathways. RNAi experiments and large-scale siRNA screens require the efficient delivery of highly functional and specific nucleic acids into the appropriate cell system, including siRNA molecules, vectors expressing shRNAs or micro RNAs. While reagent-mediated transfection is a common approach for siRNA delivery into standard cell lines, many more relevant cell types, including suspension and primary cells, are poorly accessible by such approach.  Nucleofection is an established method for the effective, non-viral transfection of nucleic acids into difficult-to-transfect cell types including primary cells. With the expansion of the method to the 96-well format high throughput applications such as siRNA-library screenings can be performed in these cell types rendering target validation and identification possible in cell types highly relevant for medical research.  We here present data showing the efficient RNAi-mediated gene knockdown in various cell lines and primary cells using 96-well nucleofection.  For example, data about the knockdown of the Fas-mediated apoptosis in Jurkat cells will be presented. The analysis was expanded to PLK1, which is a key regulator of mitotic progression in mammalian cells. Knockdown of PLK1 resulted in impaired proliferation and increased apoptosis. Starting from these single gene analyses RNAi-library screens are set up for the identification of anti-proliferative and pro-apoptotic genes.
Safety and immunogenicity of 2-dose heterologous Ad26.ZEBOV, MVA-BN-Filo Ebola vaccination in healthy and HIV-infected adults: A randomised, placebo-controlled Phase II clinical trial in Africa
Authors: Houreratou Barry, Gaudensia Mutua, Hannah Kibuuka, Zacchaeus Anywaine, Sodiomon B. Sirima, Nicolas Meda, Omu Anzala, Serge Eholie, Christine Bétard, Laura Richert, Christine Lacabaratz, M. Juliana McElrath, Stephen De Rosa, Kristen W. Cohen, Georgi Shukarev, Cynthia Robinson, Auguste Gaddah, Dirk Heerwegh, Viki Bockstal, Kerstin Luhn, Maarten Leyssen, Macaya Douoguih, Rodolphe Thiébaut
Journal: PLoS Medicine (2021)

We investigated safety, tolerability, and immunogenicity of the heterologous 2-dose Ebola vaccination regimen in healthy and HIV-infected adults with different intervals between Ebola vaccinations.