tbuckley/cabot-search huggingface dataset:
Abstract 16446: Human Mesenchymal Stem Cells of Adult and Fetal Origin Do Not Express ACE2 and are Immune to SARS-CoV-2 Spike Pseudotyped Virus
Authors: Francesca Bastaroli, Manuela Mura, Giulia Nykjaer, Massimiliano Gnecchi
Journal: Circulation (2020)

Background: Angiotensin-converting enzyme 2 (ACE2) expression in the lung has a protective role against injury. However, ACE2 is the main host cell receptor for the Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) entry. Human mesenchymal stem cells (hMSCs) are currently under investigation for the treatment of pulmonary and cardiac complications of the SARS-CoV-2 disease (COVID-19). However, it is unclear if hMSCs express ACE2 and if they possibly can secrete ACE2. Also, their predisposition to be infected by SARS-CoV-2 is unknown. Aim: To assess if hMSCs of fetal and adult origin constitutively express and secrete ACE2 and if they can be infected by SARS-CoV-2. Methods: We studied 8 hMSC lines: 4 of fetal origin isolated from human placentas (hA-MSC) and 4 of adult origin isolated from bone marrow aspirates (hBM-MSCs). The lung epithelial cancer cell line CALU-3, which expresses high levels of ACE2, was used as positive control. ACE2 expression was evaluated in both standard culture conditions and after 48 hrs of hypoxia exposure (1% O 2 ). We quantified the mRNA levels by RT-qPCR and verified protein content by western blot on both cell lysates and serum-free 48 hrs-conditioned media (MSC-CM). ACE2 levels in MSC-CM were quantified by ELISA assay. Finally, to test MSC viral susceptibility, we produced replication-defective, GFP-tagged retroviral particles bearing the SARS-CoV-2 Spike protein; the pantropic VSV glycoprotein (VSV-G) was used as positive control. Results: ACE2 mRNA level in both fetal and adult MSC was 200-fold lower than in CALU-3. ACE2 protein expression was undetectable in MSC lysates and CM, both by western blot and ELISA. Hypoxia slightly increased ACE2 mRNA level, but not protein level, which still was undetectable. Finally, CALU-3 but not MSC were infected by SARS-CoV-2 Spike pseudovirus, whereas both cell types were susceptible to VSV-G pseudovirus infection. Conclusions: Both fetal and adult MSC do not express significant levels of ACE2 both under normoxia and hypoxia, and therefore the putative therapeutic mechanism of hMSCs in COVID-19 cannot go through the release of ACE2. Importantly, MSCs are immune to SARS-CoV-2 infection and therefore their use appears safe.
Maternal exposures and the infant gut microbiome: a systematic review with meta-analysis
Authors: Allison Grech, Clare E Collins, Andrew Holmes, Ravin Lal, Kerith Duncanson, Rachael Taylor, Adrienne Gordon
Journal: Gut Microbes (2021)

Early life, including the establishment of the intestinal microbiome, represents a critical window of growth and development. Postnatal factors affecting the microbiome, including mode of delivery, feeding type, and antibiotic exposure have been widely investigated, but questions remain regarding the influence of exposures
Impact of continuous positive airway pressure therapy on metabolic syndrome in patients with obstructive sleep apnea syndrome
Authors: Shimozato Takashi, Osada Naohiko, Kida Keisuke, Yoneyama Kihei, Kou Seishou, Tsuchiya Ayako, Izumo Masaki, Ishibashi Yuki, Sekiduka Hiromitsu, Hayashi Akio, Takahashi Eiji, Suzuki Kengo, Tamura Masachika, Akashi Yoshihiro, Inoue Koji, Omiya Kazuto, Miyake Fumihiko, Izawa Kazuhiro, Watanabe Satoshi
Journal: Circulation (2008)
巨大popliteal venous aneurysmによる肺塞栓をきたした1例
Authors: Otani Hayato, Jinnai Toshikazu, Yokoyama Taku, Kawaguchi Yoshitaka, Takeuchi Ryosuke, Murata Koichiro, Shimane Akira, Onodera Tomoya, Takizawa Akinori
Journal: Circulation (2002)
Priorities for COVID-19 research response and preparedness in low-resource settings
Authors: Alice Joan Norton, Charles Shey Wiysonge, Jean-Marie Vianney Habarugira, Nicholas John White, Marta Tufet Bayona, Hans-Eckhardt Hagen, Julie Elise Archer, Moses Sifuma Alobo, Gail Lindsay Carson, Patricia Jannet Garcia, Rui M.B. Maciel, Uma Ramakrishnan, Choong-Min Ryu, Helen Rees, Francine Ntoumi, Akhona Nontanda Tshangela, Mohammad Abul Faiz, Valerie Anne Snewin, Sheila Wambui Mburu, Rachel Elizabeth Esther Miles, Brenda Gloria Amo Okware, Richard Vaux, Stefanie Sowinski, Caesar Alimsinya Atuire, Charu Kaushic
Journal: The Lancet (2021)

COVID-19 poses particular threats in low-resource settings, which typically have underfunded health-care systems and insufficient influence on the global health research agenda. Leadership and coordination have been shown in COVID-19 research in such settings, particularly across Africa, building on earlier experience from research on diseases such as Ebola virus disease and HIV. However, global coordination to achieve a coherent research response and ensure sufficient context-specific research has been challenging.
Relationship of Echo-Attenuated Plaque to Optical Coherence Tomography Findings and Occurrence of Periprocedural (Type-IVa) Myocardial Infarction in Stable Angina Pectoris
Authors: Kimura Shigeki, Shigeta Takatoshi, Kojima Keisuke, Hishikari Keiichi, Osaka Yuki, Yamao Kazuya, Kawaguchi Naohiko, Nakashima Emiko, Akiyama Daiki, Sugiyama Tomoyo, Handa Keita, Watari Yuji, Takigawa Masateru, Ohkubo Kenji, Takagi Katsumasa, Kuwahara Taishi, Hikita Hiroyuki, Takahashi Atsushi, Isobe Mitsuaki
Journal: Circulation (2013)
Abstract 17061: Impact of Maternal Anxiety on <i>In Vivo</i> Placental Development in Fetuses With Congenital Heart Disease
Authors: Yuanchiao Lu, Kushal Kapse, Nicole Andersen, Nickie Andescavage, Andrea Fry, Catherine Lopez, Anita Krishnan, Dorothy Bulas, Mary Donofrio, Catherine Limperopoulos
Journal: Circulation (2020)

Introduction: Studies have shown that maternal stress and anxiety during pregnancy have been associated with a higher incidence of preterm birth and low birth weight. However, the relationship between prenatal maternal mental distress and placental development in fetuses with congenital heart disease (CHD) is unknown. Hypothesis: We tested the hypothesis that elevated maternal anxiety is associated with altered placental morphology in fetuses with CHD. Methods: A total of 141 pregnant women were recruited prospectively (control: 91; two-ventricle/2V CHD: 29; single-ventricle/SV CHD: 21), in which 228 fetal MR scans were performed (gestational age: 31.8±4.5 weeks). Single shot fast spin echo T2-weighted images were acquired on a 1.5 Tesla GE MRI scanner. Placenta tissue was manually segmented, and slice-to-volume registration was used for motion correction and 3D reconstruction. Six placental features were calculated: volume, thickness, elongation, surface area, mean diameter, and umbilical cord centricity. We completed the Spielberger State (SSAI) and Trait (SSAI) Anxiety Inventory at each study visit. Linear mixed models were utilized to analyze the placental features predicted by maternal anxiety. Results: Placental volume, surface area, and mean diameter were decreased with elevated SSAI in 2V CHD fetuses, while volume and cord centricity were increased with higher SSAI in SV CHD fetuses. Lower cord centricity was associated with elevated STAI in 2V CHD fetuses, and elongation was positively associated with STAI in SV CHD fetuses. There were no associations between anxiety measures and placental features in control fetuses. Conclusions: We report for the first time that elevated prenatal maternal anxiety is associated with altered in vivo placental structures in pregnancies complicated by fetal CHD. Our data suggest that disturbances in placental structure vary based on the type of fetal CHD (SV vs. 2V) in the setting of elevated prenatal maternal anxiety.
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma
Authors: Ana Silva, Afonso R. M. Almeida, Ana Cachucho, João L. Neto, Sofie Demeyer, Mafalda de Matos, Thea Hogan, Yunlei Li, Jules Meijerink, Jan Cools, Ana Rita Grosso, Benedict Seddon, João T. Barata
Journal: Blood (2021)
Abstract P159: Factors Associated With Moderate Physical Activity Among Older Adults With Atrial Fibrillation: SAGE-AF Study
Authors: Jordy Mehawej, Jane Saczynski, Catarina I Kiefe, Eric Ding, Hawa O Abu, Darleen Lessard, Robert H Helm, Benita Bamgbade, Connor Saleeba, Weijia Wang, David D McManus, Robert Goldberg
Journal: Circulation (2021)

Objective: Guidelines encourage patients with atrial fibrillation (AF) to engage in moderate-intensity physical activity. We examined the sociodemographic, geriatric, and clinical factors associated with engagement in moderate-intensity physical activity among older adults with AF. Methods: Patients aged 65 years and older, diagnosed with AF, and having a CHA 2 DS 2 -VASc ≥ 2 were recruited from multiple clinics in Massachusetts and Georgia between 2015 and 2018. We utilized The Minnesota Leisure Time Physical Activity questionnaire to assess whether participants reported being engaged in moderate physical exercise. We examined the association between sociodemographic, clinical, geriatric elements and engaging in moderate-intensity physical activity using multiple logistic regression modelling. Results: The mean age of participants was 76 years and 52% were men. Approximately one-half (52%) of participants met the recommended levels of physical activity. Participants who were cognitively impaired (adjusted OR [aOR]=0.74; 95% CI= 0.56-0.97 ) , morbidly obese (aOR= 0.40; 95% CI= 0.22-0.72), socially isolated (aOR= 0.58; 95% CI= 0.40-0.84), and had a slow gait speed (aOR= 0.44; 95% CI= 0.32-0.60) were less likely, while those with higher AF related quality of life score (aOR=1.66; 95% CI=1.26-2.18) more likely, to engage in moderate physical activity. Conclusions: A considerable proportion of older adults with AF did not engage in moderate physical activity. Cognitive impairment, morbid obesity, social isolation, and slow gait speed were associated with not engaging in moderate exercise. Health care providers should identify patients with AF who are sedentary, promote engagement, and tailor interventions to address obstacles in engagement in physical activity.
12P The RODILIA pilot study for molecular screening of patients with metaplastic breast cancer
Authors: M. Silvestri, A. Vingiani, L. De Cecco, A. Belfiore, E. Ortolan, S. Veneroni, A. Trama, V. Cappelletti, G. Pruneri, S. Di Cosimo
Journal: Annals of Oncology (2021)
Methodology for characterization of platinum-based drug's targeted delivery nanosystems
Authors: J. Zajda, A. Wróblewska, L. Ruzik, M. Matczuk
Journal: Journal of Controlled Release (2021)

Conventional anticancer therapies exploiting platinum-based drugs rely principally on the intravascular injection of the therapeutic agent. The anticancer drug is distributed throughout the body by the systemic blood circulation undergoing cellular uptake, rapid clearance and excretion. Consequently, only a small portion of the platinum-based drug reaches the tumor site, which is associated with severe side effects. For this reason, targeted delivery systems are of great need since they offer enhanced and selective delivery of a drug to cancerous cells making the therapy safe and more effective. Up to date, a variety of the Pt-based drug targeted delivery systems (Pt-based DTDSs) utilizing nanomaterials have been developed and tested using a range of analytical techniques that provided essential information on their synthesis, stability, biodistribution and cytotoxicity. Here we summarize those experimental techniques indicating their applicability at different stages of the research, as well as pointing out their strengths, advantages, drawbacks and limitations. Also, the existing strategies and approaches are critically reviewed with the objective to reveal and give rise to the development of the analytical methodology suitable for reliable Pt-based DTDSs characterization which would eventually result in novel therapies and better patients' outcomes.
Abstract 17259: Identification of Biological Pathways of Candidate Atrial Fibrillation Risk Genes Through the Use of Weighted Gene Coexpression Network Analysis
Authors: Sojin Y Wass, Catherine Cantlay, Meghan McHale, Julie H Rennison, Han Sun, Jeffery Hsu, Erik Offerman, A Gillinov, Christine S Moravec, John Barnard, Jonathan D SMITH, David R Van Wagoner, Mina K Chung
Journal: Circulation (2020)

Introduction: Over 135 genetic loci have been linked to atrial fibrillation (AF), yet the biological pathways of AF pathophysiology remain elusive. Weighted gene coexpression network analysis (WGCNA) constructs gene modules within a network based on correlations in gene expression, and identifies mechanisms related to AF risk. Objective: To identify biological pathways of candidate AF risk genes that will advance our understanding of AF mechanisms. Methods: RNA-sequencing was performed on left atrial appendage tissue from 265 patients. RNA-seq data were adjusted for differences in AF rhythm state and other known AF risk factors. Correlations from adjusted data were further adjusted for latent factors then spatial quantile normalized to correct for mean-variance bias. WGCNA was applied to the resulting adjusted and normalized gene-gene correlations to identify gene modules. Ingenuity Pathway Analysis and gene set over representation analysis (GSOR) were applied to each module. Results: WGCNA identified 63 modules from 17,434 genes; 47 of these contained at least one candidate AF risk gene. AF risk genes were overrepresented in 7 modules (Table 1). Notable top pathways of AF overrepresented modules include apelin signaling, heme metabolism, intracellular ion homeostasis, and the unfolded protein response. These are known to be involved in calcium signaling, iron homeostasis, glucose regulation, heat shock response, and protein ubiquitination during states of high energy demand and stress. These pathways coincide with larger cellular processes of myocyte remodeling, apoptosis, and cell survival, which were also prominent. Conclusions: Biological pathways identified through WGCNA and GSOR suggest that sustained increases in energy demand during AF promotes stress-induced cellular remodeling. Changes in calcium signaling, iron homeostasis, the unfolded protein response and glucose regulation are likely primary mechanisms of AF pathophysiology.
176P Breast cancer treatment during the first wave of the COVID-19 pandemic at a UK centre
Authors: K. Balachandran, J. Williams, D. Bell, A. Brown, S. Mahmoud, P. Hurhangee, R. Ramakrishnan, S. Cleator, R.C. Coombes, O. Hatcher, F. Rehman, J. Stebbing, L. Kenny
Journal: Annals of Oncology (2021)
Impacts of Sugarcane Fires on Air Quality and Public Health in South Florida
Authors: Holly K. Nowell, Charles Wirks, Maria Val Martin, Aaron van Donkelaar, Randall V. Martin, Christopher K. Uejio, Christopher D. Holmes
Journal: Environmental Health Perspectives (2022)

Background: Preharvest burning of sugarcane is a common agricultural practice in Florida, which produces fine particulate matter [particulate matter (PM) with aerodynamic diameter ≤2.5μm (PM2.5)] that is associated with higher mortality. Objectives: We estimated premature mortality associated with exposure to PM2.5 from sugarcane burning in people age 25 y and above for 20 counties in South Florida. Methods: We combined information from an atmospheric dispersion model, satellites, and surface measurements to quantify PM2.5 concentrations in South Florida and the fraction of PM2.5 from sugarcane fires. From these concentrations, estimated mortalities attributable to PM2.5 from sugarcane fires were calculated by census tract using health impact functions derived from literature for six causes of death linked to PM2.5. Confidence intervals (CI) are provided based on Monte Carlo simulations that propagate uncertainty in the emissions, dispersion model, health impact functions, and demographic data. Results: Sugarcane fires emitted an amount of primary PM2.5 similar to that of motor vehicles in Florida. PM2.5 from sugarcane fires is estimated to contribute to mortality rates within the Florida Sugarcane Growing Region (SGR) by 0.4 death per 100,000 people per year (95% CI: 0.3, 1.6 per 100,000). These estimates imply 2.5 deaths per year across South Florida were associated with PM2.5 from sugarcane fires (95% CI: 1.2, 6.1), with 0.16 in the SGR (95% CI: 0.09, 0.6) and 0.72 in Palm Beach County (95% CI: 0.17, 2.2). Discussion: PM2.5 from sugarcane fires was estimated to contribute to mortality risk across South Florida, particularly in the SGR. This is consistent with prior studies that documented impacts of sugarcane fire on air quality but did not quantify mortality. Additional health impacts of sugarcane fires, which were not quantified here, include exacerbating nonfatal health conditions such as asthma and cardiovascular problems. Harvesting sugarcane without field burning would likely reduce PM2.5 and health burdens in this region. https://doi.org/10.1289/EHP9957
Abstract 16374: Factors Associated With Hypertension Prevalence Among US Adults With Diabetes Mellitus: The National Health and Nutrition Examination Survey 2015-2016
Authors: Joel C Marrs, Sarah L Anderson
Journal: Circulation (2018)

Introduction: National prevalence data on diabetes (DM) and hypertension (HTN) based on the National Health and Nutrition Examination Survey (NHANES) 2015-2016 cohort has not been reported on and i...
Abstract 16807: The Association Between Dietary Factors and the Distribution of Monocyte Subsets: A Systematic Review
Authors: Ester Oh, Muzi Na, Connie Rogers
Journal: Circulation (2020)

Introduction: Three subsets of monocytes have been identified in humans (i.e. classical, intermediate and non-classical), which have been proposed to differently contribute to the pathogenesis of atherosclerosis, a major cause of cardiovascular disease (CVD). Previous studies implicate a shift in the distribution of monocyte subsets from classical (CM) to intermediate (IM) and non-classical monocytes (NCM) in individuals with cardiometabolic disorders, which is hypothesized to contribute to CVD development. The risk of cardiometabolic disease is strongly associated with dietary habits. Thus, the aim of the current systematic review was to evaluate the association between dietary factors and the distribution of monocyte subsets. Methods: Articles were systematically searched in CINAHL, Cochrane Central Register of Controlled Trials, and PubMed until May 2020. A total of 2496 articles were independently screened by 2 reviewers. Eligible study subjects were adults &gt; 18 years old. Eligible studies were cross-sectional, cohort, within-subject design studies and randomized-controlled trials that examine the effect of dietary factors on monocyte subset distribution. A total of 12 studies were included in this review. Results: A single high fat meal challenge increased the number and percentage of NCM (n=1) and increased the number and percentage of CM (n=1) in healthy subjects. Consuming a high-salt diet for a week increased the number and percentage of IM in healthy subjects, whereas these changes were reversed after switching to a low-salt diet (n=2). EPA+DHA supplementation (4 g/day) for 2 weeks decreased the number and percentage of IM in patients with hypertriglyceridemia (n=2). Lastly, one cross-sectional study showed that the number and percentage of IM was lower in patients with CVD who consumed a high quality diet compared to low quality diet. Conclusions: Dietary factors may influence the distribution of monocyte subsets. However, the role of diet-induced changes in the distribution of monocytes on atherosclerosis risk remains unknown. Additional mechanistic studies are needed to understand the relationship between dietary factors, changes in the distribution of monocyte subsets and atherosclerotic risk.
Abstract P014: Life’S Simple 7 And Fibroblast Growth Factor 23: The Boston Puerto Rican Health Study.
Authors: Chinedu C Ochin, Sherman Bigornia, Mahdi O Garelnabi, Katherine L Tucker
Journal: Circulation (2021)

Introduction: FGF-23 is a phosphatouric hormone which also serves as a plasma biomarker that mediates pathologic cardiac remodeling, such as left ventricular hypertrophy or myocardial fibrosis, and is associated with increased cardiovascular mortality. However, limited evidence is available on lifestyle-related factors associated with circulating concentration of this emerging cardiovascular risk factor. Objective: Our aim was to measure the association between the American Heart Association’s Life’s Simple 7 (LS7) and circulating FGF-23 concentration among Puerto Rican adults. Methods: Data are from Boston Puerto Rican Health Study (BPRHS) participants (mean age 56.2 years, 77% female) at baseline and at approximately 8-y follow-up (n=410). LS7 was calculated at baseline and includes 4 physiological (BMI, total cholesterol, blood pressure, and fasting glucose) and 3 behavioral factors (smoking, physical activity, and diet). Each component was categorized using a constituent scoring system (ideal=2; intermediate=1; Poor=0) and these were summed to calculate the total LS7 score. Participants were categorized into 5 LS7 groups as follows: 1) score 1-4 (n=105), 2) 5 (n=76), 3) 6 (n=89), 4) 7 (n=69), and 5) 8-12 (n=71). Fasting plasma FGF-23 concentration at 8-y follow-up was measured by ELISA. Associations between baseline LS7 score and 8-y FGF-23 were assessed using ANCOVA, adjusted for age and sex (model 1), and additionally for history of cardiovascular disease and poverty-income-ratio (model 2). Results: Relative to participants in LS7 group 1 (2.98 ± 1.09 mg/dL), those in groups 5 (1.63 ± 1.12 mg/dL, p=0.0001) had significantly lower FGF-23 concentration (model 1, P-trend=0.0001). After considering additional confounders, FGF-23 concentration among LS7 group 5 (1.69 ± 1.12 mg/dL, p=0.0007) remained significantly lower than in group 1 (2.95 ± 1.09 mg/dL). The p-trend across categories was also significant at p=0.0007. In additional analyses, LS7 components for smoking (ideal vs. poor, 2.07 ± 1.10 mg/dL vs. 2.87 ± 1.10 mg/dL, p=0.01) and fasting glucose (ideal vs. poor, 2.03 ± 1.07 mg/dL vs. 2.90 ± 1.09 mg/dL, p=0.004), but no other components, were associated with FGF-23 concentration. Conclusions: Optimal LS7 status was associated with lower 8-y mean plasma FGF-23 concentration. Efforts to target LS7 components, particularly smoking and glucose control, may improve FGF-23 concentration among Puerto Rican adults.