tbuckley/cabot-search huggingface dataset:
POS0399 TMT-BASED QUANTITATIVE PROTEOMICS ANALYSIS OF SYNOVIAL FLUID-DERIVED EXOSOMES IN RHEUMATOID ARTHRITIS, AXIAL SPONDYLOARTHRITIS, GOUT AND OSTEOARTHRITIS
Authors: Y. Liu, Y. Huang, Q. Huang, Z. Huang, Z. Li, T. W. Li
Journal: Annals of the Rheumatic Diseases (2021)

Background: The pathogeneses of the joint diseases rheumatoid arthritis (RA), axial spondyloarthritis (axSpA), gout, and osteoarthritis (OA) are still not fully elucidated. Exosomes in synovial fluid (SF) has a critical role in the pathogenesis of arthritis. None of study has compared the proteomics of SF-derived exosomes in RA, axSpA, gout and OA. Objectives: To compare the proteomics of SF-derived exosomes in RA, axSpA, gout and OA based on tandem mass tags (TMT) labeled quantitative proteomics technique. Methods: SF-derived exosomes was isolated from RA, axSpA, gout and OA patients by the Exoquick kit combined ultracentrifugation method. TMT labeled quantitative proteomics technique was used to compare the proteomics of SF-derived exosomes. Volcano plot, hierarchical cluster, Gene Ontologies (GO), Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted. Results: A total of 1678 credible proteins were detected. With the cut off criteria of |log2 (fold-change)| ≥1.2 and p-value &lt;0.05, 267 (140 up-regulated and 127 down-regulated)differential proteins were found in OA vs gout, 291 (179 and 112) in axSpA vs OA, 515 (109 and 406) in RA vs axSpA, 298 (191 and 107) in axSpA vs gout, 462 (160 and 302) in RA vs gout, 536 (170 and 366) in RA vs OA. GO analysis showed that the biological progress of differential proteins were mainly enriched in the “immune response”. Regarding the molecular function, the differential proteins mainly mediated “antigen binding”. GO analysis of the cellular components indicated that most proteins were annotated as “extracellular exosomes”. KEGG pathway analysis demonstrated differential proteins were significantly enriched in “complement and coagulation cascades”. The hierarchical cluster analysis of the differential proteins in the four groups showed that Lysozyme C and Keratin were more abundant in gout, Hemoglobin and Actin-related protein 2/3 complex subunit 3 in OA, Sodium/potassium-transporting ATPase subunit alpha-1 and Immunoglobulin heavy constant delta in axSpA, Pregnancy zone protein and Stromelysin-1 in RA. Conclusion: The protein profiles of SF-derived exosomes in RA, axSpA, gout and OA patients were different. The differential proteins were the potential biomarkers of RA, axSpA, gout and OA. References: [1]Cretu D, Diamandis E P, Chandran V. Delineating the synovial fluid proteome: recent advancements and ongoing challenges in biomarker research.[J]. Critical reviews in clinical laboratory sciences, 2013,50(2):51-63. [2]McArdle A J, Menikou S. What is proteomics?[J]. Archives of disease in childhood. Education and practice edition, 2020. Figure 1. The hierarchical cluster analysis of differential proteins in axSpA, OA, Gout and RA. Disclosure of Interests: None declared
Abstract 17145: Surface Modification Of Stem Cell Exosomes Myocardial Infarction Specific Peptides For Non Invasive Delivery To Ischemic Myocardium
Authors: Vandana Mallaredy, Zhongjian Cheng, Venkata Garikipati, Maria Cimini, Grace Huang, Chunlin Wang, May Trungcao, Cindy Benedict, Raj Kishore
Journal: Circulation (2019)

Exosomes are under extensive investigation as a next generation therapeutic agent for clinical applications in various diseases. Stem cell-derived exosomes have been shown to provide post-MI cardia...
International aid and covid-19 in India: a humanitarian catastrophe at risk of a familiar response
Authors: Najeeb Rahman
Journal: BMJ (2021)

Observing the unfolding tragedy in India is analogous to watching the news of a humanitarian disaster such as an earthquake or tsunami—the sheer scale necessitates an international response.1

Given this analogy to events such as the South Asian Tsunami of 2004 or the Haiti earthquake of 2010, will the “cluster coordination” approach be used to ensure that resources are distributed equitably?2 Will minimum standards of response, as outlined by Sphere,3 be followed to meet the needs of vulnerable communities? …
Petrous Apex Cholesterol Granuloma Showing 68Ga-DOTATATE Uptake
Authors: Nuh Filizoglu, Efe Soydemir, Tunc Ones, Salih Ozguven, Feyza Sen
Journal: Clinical Nuclear Medicine (2021)

Abstract The importance of 68 Ga-DOTATATE PET/CT in the evaluation of neuroendocrine tumors is well established. However, false-positive results can occur due to inflammatory pathologies. We report a 47-year-old man who was diagnosed with a neuroendocrine tumor of the stomach. Initial 68 Ga-DOTATATE PET/CT showed intense 68 Ga-DOTATATE uptake in the petrous apex. Histopathological confirmation could not be obtained, thus the asymptomatic patient was followed with periodic clinical/radiologic examinations for more than 2.5 years. Therefore, this lesion was diagnosed as a cholesterol granuloma of the temporal bone. These results suggested that an initial wait-and-scan approach is a feasible management strategy for similar presentations.
Health-related quality of life and economic burden of prurigo nodularis
Authors: Katherine A. Whang, Thomas K. Le, Raveena Khanna, Kyle A. Williams, Youkyung Sophie Roh, Nishadh Sutaria, Justin Choi, Sylvie Gabriel, Rajeev Chavda, Yevgeniy Semenov, Shawn G. Kwatra
Journal: Journal of the American Academy of Dermatology (2021)
Abstract 14400: The Association Between Bleeding and Warfarin Discontinuation in Patients with Atrial Fibrillation
Authors: Xianying Pan, Hugh Kawabata, Han-Yao Huang, Teresa A Simon, Laurent Azoulay, Flavia Dietrich, Uchenna Iloeje, Samy Suissa
Journal: Circulation (2012)

Purpose: Bleeding is an adverse event of warfarin use and may lead to warfarin discontinuation. This study aimed to quantify the association between bleeding during or resulting in a hospitalizatio...
POS1013 LET’S TAKE A LOOK AT THE SYMPHYSIS PUBIC AREA IN SPONDYLARTHRITIS
Authors: H. Hajji, H. Ferjani, K. Maatallah, S. Miri, C. Ines, M. Yasmine, D. Kaffel, W. Hamdi
Journal: Annals of the Rheumatic Diseases (2021)

Background: Symphysis pubic (SP) is the frequent site of enthesitis in spondylarthritis (SpA). Radiological changes in SP appear later in the course of the disease. Underdiagnosed, its prevalence varies from 4% to 47% (1), depending on imaging modalities. Objectives: This study aimed to evaluate the prevalence of SP involvement in patients with spondyloarthritis (SpA). We also focused on the relation between radiographical changes and clinical findings. Methods: It was a cross-sectional study, including patients with SpA according to the Assessment of SpondyloArthritis International Society (ASAS) criteria. We collected the following data: age, gender, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Ankylosing Spondylitis Disease Activity Score (ASDAS), and Bath Ankylosing Spondylitis Functional Index (BASFI). Pelvic radiographs were examined by 2 experimented rheumatologists. Grading symphysial involvement was made as follow: scores ranged from 0-4 per reading: grade 0 = normal; grade 1 = subtle irregularity and/or subchondral sclerosis, grade 2 = clear erosions, 3 = marked sclerosis, grade 4 = ankylosis. We divided our patients into two groups: G0 patients without SP changes and G1 patients with SP changes. Results: One hundred and thirty-one patients were included, 84 were male, and 48 were female. The sex ratio M/F was 1.72. The mean age was 41.32±12.42 years. The mean disease duration was 12.65 ± 9.49 years. The clinical presentation of SpA was peripheral in 61 cases and axial in 118 cases. The mean disease scores activity was: BASDAI: 3.94±2.046 and ASDASCRP: 2.75±1.05. The mean BASFI was 4.17±2.7 SP changes were observed in 31 patients: score 1 (n=14), score 2 (n=8), score 3 (n=8) and score 4 (n=4). Sex ratios M/F were 2.1 and 1.65 in G1 and G0, respectively (p=0.23). No statistically significant differences were reported between the two groups G0 and G1: mean age (40.48 vs. 43.45, p=0.324), mean disease duration (11.19 vs. 14.45, p=0.218), mean BASDAI (3.8 versus 3.9, p=0.850), mean ASDAS-ESR (3.09 vs. 2.55, p=0.113) and mean BASFI (3.76 versus 4.96, p=0.06) respectively. In G1, nine patients had hip involvement (p=0.203). Enthesitis was more common in patients with SP changes (p=0.02). Conclusion: In our study, the presence of enthesitis was associated with SP changes. Surprisingly, age and disease duration did not influence SP changes (1). References: [1]Kang Y, Ahn JM, Lee E, Lee JW, Kang HS. Active inflammatory changes around the pubic symphysis in patients with axial spondyloarthritis: Magnetic resonance imaging characteristics and association with clinical factors. Eur J Radiol. mars 2020;124:108802. Disclosure of Interests: None declared.
Promoting Trust and Morale by Changing How the Word <i>Provider</i> Is Used
Authors: John W. Beasley, Richard G. Roberts, Allan H. Goroll
Journal: JAMA (2021)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
AB0782 MONOARTHRITIS: PROBABLE OUTCOMES
Authors: M. Osipyan, M. Efraimidou, V. Vardanyan, K. Ginosyan
Journal: Annals of the Rheumatic Diseases (2021)

Background: Numerous joint disorders initially produce swelling in a single joint and new onset monoartritis will probably further lead to the involvement of other joint groups and development of extraarticular manifestations. It is essential to take a proper diagnostic approach for organizing appropriate treatment and lowering possibility of disease progression. Objectives: The aim of this study was to investigate joint distribution, determine rheumatological diseases of patients with acute monoarthritis and reveal the development of further systemic manifestations. Methods: 100 patients (age 18-75 years) with clinically apparent monoarthritis of less than 6 weeks duration were included in the study. Criteria of exclusion were infection, trauma and crystal induced arthritis. Joint distribution, presence of systemic manifestations and development of chronic inflammatory rheumatic disease were evaluated. Presence of arthritis was proved with help of ultrasound examination. Complete blood count, ESR, CRP, RF, anti-CCP; HLAB27; MEFV mutations and X-ray of swollen joint were performed for all patients. Temperature was also measured. Results: Mean age of patients with acute monoarthritis was 46±13 years. Female predominance was noted (61%). 71% of patients had elevated ESR, 69%- CRP. In 24% of cases homozygous or heterozygous mutations of MEFV gene were revealed. 21% of patients had positive RF and 18% - anti-CCP. 11% patients carried HLA-B27 antigen. 28% of examined patients had subfebril fever. Hepatosplenomegaly was determined in 16%, uveitis in 5%, psoriatic plaque in 4%, interstitial pneumonia in 2% of cases At the baseline 82 patients were diagnosed with rheumatologically disease. Baseline data is shown in the Table 1 bellow. Table 1. Baseline data Diagnosis Number of patients FMF 23 Osteoarthritis (reactive synovitis) 16 Rheumatoid arthritis 15 Reactive arthritis 10 Ankylosing spondylitis 6 Psoriatic arthritis 4 SLE 3 Schonleyn-Henoch purpura 2 Sarcoidosis 2 Behcet diseases 1 Conclusion: In this study monoarhtritis in majority of cases underlies FMF. Though FMF is not considered as a frequent cause of acute monoarthritis, more attention should be paid on this pathology in focus of monoarthritis, especially in specific for FMF region. Further follow up of acute monoarthritis progression is needed. References: [1]A. Becker, J. Daily, K. Pohlgeers. Acute Monoarthritis: Diagnosis in Adults. Am Fam Physician 2016; 94(10): 810-816 [2]S. Camacho-Lovillo, A. García-Martínez. Arthritis as presentation of familial Mediterranean fever. An Pediatr (Barc). 2015; 83(2):130. DOI: 10.1016/j.anpede.2015.07.007 [3]J. Ellis. Acute monoarthritis. JAAPA. 2019, 32(3):25-31. doi: 0.1097/01.JAA.0000553379.52389.eb Disclosure of Interests: None declared
Allocating/Rationing Organs for Transplant
Authors: Kenneth A. Andreoni
Journal: JAMA Surgery (2021)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Megakaryocyte production is sustained by direct differentiation from erythromyeloid progenitors in the yolk sac until midgestation
Authors: Lorea Iturri, Laina Freyer, Anne Biton, Pascal Dardenne, Yvan Lallemand, Elisa Gomez Perdiguero
Journal: Immunity (2021)

The extra-embryonic yolk sac contains the first definitive multipotent hematopoietic cells, denominated erythromyeloid progenitors. They originate in situ prior to the emergence of hematopoietic stem cells and give rise to erythroid, monocytes, granulocytes, mast cells and macrophages, the latter in a Myb transcription factor-independent manner. We uncovered here the heterogeneity of yolk sac erythromyeloid progenitors, at the single cell level, and discriminated multipotent from committed progenitors, prior to fetal liver colonization. We identified two temporally distinct megakaryocyte differentiation pathways. The first occurs in the yolk sac, bypasses intermediate bipotent megakaryocyte-erythroid progenitors and, similar to the differentiation of macrophages, is Myb-independent. By contrast, the second originates later, from Myb-dependent bipotent progenitors expressing Csf2rb and colonize the fetal liver, where they give rise to megakaryocytes and to large numbers of erythrocytes. Understanding megakaryocyte development is crucial as they play key functions during vascular development, in particular in separating blood and lymphatic networks.
Expansion of Fcγ Receptor <scp>IIIa</scp>–Positive Macrophages, Ficolin 1–Positive <scp>Monocyte‐Derived</scp> Dendritic Cells, and Plasmacytoid Dendritic Cells Associated With Severe Skin Disease in Systemic Sclerosis
Authors: Dan Xue, Tracy Tabib, Christina Morse, Yi Yang, Robyn T. Domsic, Dinesh Khanna, Robert Lafyatis
Journal: Arthritis & Rheumatology (2021)

In this study, we sought a comprehensive understanding of myeloid cell types driving fibrosis in diffuse cutaneous systemic sclerosis (dcSSc) skin.We analyzed the transcriptomes of 2,465 myeloid cells from skin biopsy specimens from 12 dcSSc patients and 10 healthy control subjects using single-cell RNA sequencing. Monocyte-derived dendritic cells (mo-DCs) were assessed using immunohistochemical staining and immunofluorescence analyses targeting ficolin-1 (FCN-1).A t-distributed stochastic neighbor embedding analysis of single-cell transcriptome data revealed 12 myeloid cell clusters, 9 of which paralleled previously described healthy control macrophage/DC clusters, and 3 of which were dcSSc-specific myeloid cell clusters. One SSc-associated macrophage cluster, highly expressing Fcγ receptor IIIA, was suggested on pseudotime analysis to be derived from normal CCR1+ and MARCO+ macrophages. A second SSc-associated myeloid population highly expressed monocyte markers FCN-1, epiregulin, S100A8, and S100A9, but was closely related to type 2 conventional DCs on pseudotime analysis and identified as mo-DCs. Mo-DCs were associated with more severe skin disease. Proliferating macrophages and plasmacytoid DCs were detected almost exclusively in dcSSc skin, the latter clustering with B cells and apparently derived from lymphoid progenitors.Transcriptional signatures in these and other myeloid populations indicate innate immune system activation, possibly through Toll-like receptors and highly up-regulated chemokines. However, the appearance and activation of myeloid cells varies between patients, indicating potential differences in the underlying pathogenesis and/or temporal disease activity in dcSSc.
Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells
Authors: David Wu, Devin L. Harrison, Teodora Szasz, Chih-Fan Yeh, Tzu-Pin Shentu, Angelo Meliton, Ru-Ting Huang, Zhengjie Zhou, Gökhan M. Mutlu, Jun Huang, Yun Fang
Journal: Nature Metabolism (2021)
AB0714 COMPARISON OF THE EFFECT OF SUBACROMIAL ADMINISTRATION OF HYALURONIC ACID AND PLATELET RICH PLASMA IN CASES OF DAMAGE TO THE TENDONS OF THE ROTATORS OF THE SHOULDER IN INDIVIDUALS OF DIFFERENT AGE GROUPS
Authors: V. Nesterenko, A. Karateev, E. Bialik, M. Makarov, S. Makarov, V. Bialik, M. Nurmukhametov, A. Chernikova
Journal: Annals of the Rheumatic Diseases (2021)

Background: Everyone has had shoulder pain at least once in their life. From 70 to 80% of chronic pain in the shoulder joint is associated with subacromial impingement syndrome. The use of non-steroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids does not always meet the needs of patients and have a number of adverse reactions. Local administration of PRP and HA preparations is widely used for chronic shoulder pain. However, the question of the effectiveness of these drugs in patients of different ages has been insufficiently studied. Objectives: To compare the efficacy of GLA and PRP in patients with chronic shoulder pain by age. Methods: The study group included 100 patients, 46% women and 54% men, aged 51.5 ± 15.1, with chronic shoulder pain (≥3 months) caused by damage to the tendons of the muscles of the shoulder rotators. Patients &lt;45 years old accounted for 34%, patients ≥45 years old - 66%. All patients were randomly divided into 2 groups. Patients in group 1 received 2 consecutive subacromial injections of GLK, groups 2 - 3 consecutive subacromial injections of PRP. Pain dynamics were assessed using a 100-mm visual analogue scale (VAS), functional impairments according to ASS (American Shoulder and Elbow Surgeons Assessment) and CS (Constant Score), and a decrease in the need for NSAID use after 6 months. after the course of treatment. Results: The dynamics of pain and functional disorders in the treatment of GLC and PRP did not differ. The pain decreased from 57.6 ± 17.8 to 30.2 ± 26.3 and 56.0 ± 14.6 to 31.8 ± 26.3 (p = 0.768), ASS from 54.7 ± 15.1 to 77.3 ± 22.5 and 54.8 ± 13.8 to 74.6 ± 22.4 (p = 0.552), CS from 47.8 ± 16.9 to 65.6 ± 19.3 and 59.2 ± 14.4 to 66.9 ± 17.4 (p = 0.245). In general, with the treatment of GLA and PRP, the dynamics of pain and functional disorders was significantly better in patients &lt;45 years old than in patients ≥45 years old. So, pain according to VAS after 6 months. was 22.4 ± 26.3 and 35.5 ± 26.2 (p = 0.022), ASS 83.3 ± 20.9 and 72.1 ± 22.6 (p = 0.017), CS 76.2 ± 16, 1 and 63.2 ± 18.2 (p = 0.001). There was no need to take NSAIDs in 82.4% and 65.2% of patients (p = 0.103). No serious adverse reactions have been reported with the treatment of GLC and PRP. Conclusion: The efficacy of GLA and PRP in chronic shoulder pain caused by damage to the tendons of the muscles of the rotator of the shoulder does not differ. The clinical response to both drugs was significantly higher in those under 45 years of age. Disclosure of Interests: None declared
A verrucous plaque on the hand
Authors: Fang-Gu Li, Yi-Ming Fan
Journal: BMJ (2021)

An otherwise healthy woman in her 80s presented with a two month history of an asymptomatic, rapidly growing plaque on the dorsum of her right hand. She reported no previous trauma or local treatment. Cutaneous examination showed a 2.3×1.8 cm well defined, oblong, yellowish verrucous plaque with multiple black dots and a reddish halo (fig 1). No axillary lymphadenopathy was identified. The results of systemic examination and routine blood investigations were unremarkable.



Fig 1 
Well defined, oblong, yellowish verrucous plaque (2.3×1.8 cm) with multiple black dots and reddish halo on the dorsum of the right hand



Because the results of dermoscopy were inconclusive—numerous shiny white papillae with or without central faint red-purple glomerular and linear vessels (frogspawn-like appearance) and dark brown-black haemorrhagic crusts (fig 2)—and as this was a rapidly growing plaque in …
Identification of an Altered Matrix Signature in Kidney Aging and Disease
Authors: Michael J. Randles, Franziska Lausecker, Qingyang Kong, Hani Suleiman, Graeme Reid, Maria Kolatsi-Joannou, Bernard Davenport, Pinyuan Tian, Sara Falcone, Paul Potter, Tom Van Agtmael, Jill T. Norman, David A. Long, Martin J. Humphries, Jeffrey H. Miner, Rachel Lennon
Journal: Journal of the American Society of Nephrology (2021)

Accumulation of extracellular matrix in organs and tissues is a feature of both aging and disease. In the kidney, glomerulosclerosis and tubulointerstitial fibrosis accompany the decline in function, which current therapies cannot address, leading to organ failure. Although histologic and ultrastructural patterns of excess matrix form the basis of human disease classifications, a comprehensive molecular resolution of abnormal matrix is lacking.