FRI0171 Levels of soluble vcam-1, p-selectin, and e-selectin in patients with primary antiphospholipid syndrome and systemic lupus erytematosus
Authors: AA Novikov, EN Alexandrova, TM Reshetnyak
Journal: Annals of the Rheumatic Diseases (2001)

<h3>Background</h3> The antiphospholipid antibodies (aPL) are known to modify the expression of adhesion molecules on activated endothelial cells and platelets <i>in vitro</i> and <i>in vivo</i>. <h3>Objectives</h3> <h3>Methods</h3> We studied the levels of soluble (s) P-selectin, sE-selectin, and sVCAM-1 by ELISA in sera from 19 patients (pts) (5 male, 14 female, mean age? 36.9 ± 10.1 years) with primary antiphospholipid sindrome (PAPS), 23 pts (10 male, 13 female, mean age ? 35.3 ± 11.6 years) with systemic lupus (SLE) erythematosus? related antiphospholipid sindrome (SLE-APS), 15 pts (13 male, 2 female, mean age? 29.0 ± 9.0 years) with SLE and 38 controls. <h3>Results</h3> The sE-selectin and sVCAM-1 concentrations were significantly higher in pts with PAPS, SLE-APS and SLE compared with controls. (78.3 ± 61.2 ng/ml; 52.3 ± 14.1 ng/ml; 70.5 ± 46.9 ng/ml vs 38.0 ± 13.4 ng/ml; p = 0.0019, p = 0.0363, p = 0.0144, respectively for sE-selectin; 1003 ± 582.6 ng/ml; 1189.6 ± 691.0 ng/ml; 1227 ± 680.2 ng/ml vs 594.7 ± 63.3 ng/ml; p = 0.0001 for sVCAM-1). The sP-selectin concentration was significantly higher in pts with SLE compared with controls (262.4 ± 158.8 ng/ml vs 120.1 ± 53.7 ng/ml; p = 0.0037). Within the group of pts with PAPS the sP-selectin concentration was higher among those with ischaemic CNS involvement. (p &lt; 0.02). The serum C-reactive protein level was positively correlated with the sE-selectin concentration in SLE-APS (r = 0.341, p &lt; 0.05) and SLE (r = 0.699, p &lt; 0.01), the sVCAM-1 concentration in PAPS (r = 0.705, p &lt; 0.001) and SLE (r = 0.405, p &lt; 0.05). The serum, anti ds and ssDNA antibody level was positively correlated with the sP-selectin concentration in SLE-APS (r = 0.730, and r = 0.909, p &lt; 0.001), and the sE-selectin concentration in SLE (r = 0.684, p &lt; 0.001 and r = 0.616, p &lt; 0.002). <h3>Conclusion</h3> The sE-selectin and sVCAM-1 concentrations were significantly higher in pts with PAPS, SLE-APS and SLE compared with controls. (78.3 ± 61.2 ng/ml; 52.3 ± 14.1 ng/ml; 70.5 ± 46.9 ng/ml vs 38.0 ± 13.4 ng/ml; p = 0.0019, p = 0.0363, p = 0.0144, respectively for sE-selectin; 1003 ± 582.6 ng/ml; 1189.6 ± 691.0 ng/ml; 1227 ± 680.2 ng/ml vs 594.7 ± 63.3 ng/ml; p = 0.0001 for sVCAM-1). The sP-selectin concentration was significantly higher in pts with SLE compared with controls (262.4 ± 158.8 ng/ml vs 120.1 ± 53.7 ng/ml; p = 0.0037). Within the group of pts with PAPS the sP-selectin concentration was higher among those with ischaemic CNS involvement (p &lt; 0.02). The serum C-reactive protein level was positively correlated with the sE-selectin concentration in SLE-APS (r = 0.341, p &lt; 0.05) and SLE (r = 0.699, p &lt; 0.01), the sVCAM-1 concentration in PAPS (r = 0.705, p &lt; 0.001) and SLE (r = 0.405, p &lt; 0.05). The serum, anti ds and ssDNA antibody level was positively correlated with the sP-selectin concentration in SLE-APS (r = 0.730, and r = 0.909, p &lt; 0.001), and the sE-selectin concentration in SLE (r = 0.684, p &lt; 0.001 and r = 0.616, p &lt; 0.002).