tbuckley/cabot-search huggingface dataset:
AB1210 THE IMPACT OF EXAMINATION STRESS ON AUTOIMMUNE DISEASES AMONG UNIVERSITY STUDENTS
Authors: F. Fayed, E. Abdelkarim, M. Morsy
Journal: Annals of the Rheumatic Diseases (2020)

Background: Stress is a risk factor of various diseases including autoimmune diseases. Autoimmune diseases are one of the leading causes of morbidity in young adults. (1) Examination stress is a main concern nowadays due to the study style, lack of preparation, doctor- student relationship and family pressure. (2) The previous studies declared that stress may causes neuroendocrinal changes leading to immune dysregulations and cytokines production. (3) Objectives: The aim of study is to scope the light on the importance of stress as a predisposing factor in autoimmune disease flares particularly Examination stress. Methods: A three-year (2017-2019) cross-sectional prospective study conducted on 1365 students who presented to the Alexandria University rheumatology clinic during examinations. Clinical assessments, routine investigations, activity markers, activity indices, stress and anxiety questionnaires and perceived stress scale (PSS) were applied to all patients during consecutive visits. Results: Through 5800 visits in three years during examination sessions, patients age ranged from (17 -25) years with 76% females and 24% males. They grouped into SLE (31.35%), Rheumatoid arthritis (RA) (37.28%), Fibromyalgia (13.91%), FMF (2.63%), Ankylosing Spondylitis (1.75%), Psoriatic arthritis (0.73%), systemic sclerosis (0.58%), and undifferentiated connective tissue (11.73%). According to SLE patients, 43.92% were newly diagnosed whilst 54.16% of previously diagnosed SLE presented with Flare in particular lupus nephritis (56.33%), arthritis (43.22%), hematological (49.76%) and serositis (21.36%). Interestingly, RA patients who newly diagnosed were 35.16% of total RA patients while 42.42% of previously diagnosed RA patients presented with moderate and high DAS-28 due to incompliance with treatment in (64.37%) of patients, (11.53% on biological, 88.47% on conventional treatment). In addition, (49.36%) of FMF presented in recent attacks. It was also found that Arthralgia, bone aches and sleep deprivation are the main complaints. Concerning, A High perceived stress scale (PSS) was associated with High DAS28 and SLEDI-2K scores. (r s = 0.723, 0.865) (P&lt;0.001) Conclusion: Examination stress is one of triggering factor for autoimmune disease flares. It is associated with high disease activities and ruthless outcomes. References: [1]Cooper, G. S., &amp; Stroehla, B. C. The epidemiology of autoimmune diseases. Autoimmunity Reviews,2003:2(3);119–25. [2]Archana kumari, jagrati jain. Examination stress and anxiety: a study of college students. Global Journal of Multidisciplinary Studies 2014:4:ISSN 2348-0459 Disclosure of Interests: None declared
Absence of Influence of the Pre-Transplant Immune Status of Recipients on Survivals and Gvhd after Allogeneic Stem Cell Transplantation: A Retrospective Study of 195 Cases
Authors: Baptiste Le Calvez, Thierry Guillaume, Pierre Peterlin, Alice Garnier, Amandine Le Bourgeois, Marie Audrain, Marion Eveillard, Thomas Dejoie, Béatrice Mahé, Viviane Dubruille, Nicolas Blin, Cyrille Touzeau, Thomas Gastinne, Anne Lok, Benoit Tessoulin, Sophie Vantyghem, Steven Le Gouill, Philippe Moreau, Marie C Bene, Patrice Chevalier
Journal: Blood (2020)
CD38-Directed CAR-T Cell Therapy: A Novel Immunotherapy Strategy for Relapsed Acute Myeloid Leukemia after Allogeneic Hematopoietic Stem Cell Transplantation
Authors: Xiaowen Tang, Depei Wu, Qingya Cui, Chongsheng Qian, Nan Xu, Liqing Kang, Haiping Dai, Baoquan Song, Wei Cui, Jia Yin, Zheng Li, Lei Yu
Journal: Blood (2020)
Clinical Outcomes of Patients with Secondary Acute Myeloid Leukemia (sAML) Treated with Hypomethylating Agent Plus Venetoclax (HMA-Ven) or Liposomal Daunorubicin Cytarabine (CPX-351)
Authors: Amandeep Salhotra, Dat Ngo, Jianying Zhang, Karamjeet S. Sandhu, Monzr M. Al Malki, Ahmed Aribi, Haris Ali, Pkoller Koller, Shukaib Arslan, Andrew S. Artz, Lihua E Budde, Samer Khaled, Sanjeet S Dadwal, David S Snyder, Stephen J. Forman, Ryotaro Nakamura, Anthony S. Stein, Guido Marcucci, Ibrahim Aldoss, Vinod A. Pullarkat
Journal: Blood (2020)
Intention to Treat Analysis of Real-World Outcomes Following Tisgenlecleucel Therapy for Pediatric and Young Adult ALL through a National Access Programme
Authors: Sara Ghorashian, Caroline Furness, Michelle Cummins, John A Snowden, Maeve A O'Reilly, Claire Roddie, Lorna Neill, Emma Nicholson, Caroline Besley, Sridhar Chaganti, John Gillson, Antonio Pagliuca, Anna Castleton, Amit Patel, Peter Clark, Robert Fernley, Robert Wynn, Ajay Vora, David I. Marks, Geoff Shenton, Persis J Amrolia, Denise Bonney, Rachael E Hough
Journal: Blood (2020)
FRI0074 Adenoviral-based overexpression of timp-1 reduces tissue damage in the joints of tnf-a-transgenic mice
Authors: G Schett, S Hayer, M Tohidast-Akrad, Q Xu, G Kollias, G Steiner, J Smolen
Journal: Annals of the Rheumatic Diseases (2001)

<h3>Background</h3> Rheumatoid arthritis is a prototype of a destructive inflammatory disease. Inflammation triggered by the overexpression of TNF-a is a driving force of this disorder and mediates tissue destruction. The particular impact of TNF-a-induced molecules, such as matrix metalloproteinases, in tissue destruction is however unknown. <h3>Objectives</h3> Herein, the effect of an overexpression of tissue inhibitor of metalloproteinases (TIMP)-1, a physiological antagonist of metalloproteinases, was studied in the arthritis model of TNF- a-transgenic mice. <h3>Methods</h3> Systemic treatment was carried out by replication-defective adenoviral vectors for TIMP-1 (AdvTIMP1, n = 7) or b-galactosidase (AdvLacZ, n = 6) or phosphate buffered saline (PBS, n = 7), which were applied once at the onset of arthritis. Clinical, serological, radiological and histological outcomes were assessed 18 days after the treatment. <h3>Results</h3> The AdvTIMP1 group showed a significantly reduced paw swelling and increased grip strength compared to the two control groups, whereas total body weight, TNF-a and IL-6 levels were similar in all three groups. Radiological assessment revealed a significant reduction of joint destruction in the AdvTIMP1 group; this was confirmed by histological analyses, showing reduced formation of pannus as well as erosions in the AdvTIMP1 group as compared to the AdvLacZ- and PBS- control groups. The formation of arthritis-specific autoantibodies to hnRNP-A2 was not observed in the AdvTIMP1 group but was present in the two control groups. <h3>Conclusion</h3> These results indicates a central role of matrix metalloproteinases in TNF-a-mediated tissue damage <i>in vivo</i> and a promising therapeutic role of TIMP-1.
Thigh pain after a stroke
Authors: Valencia Long, Sheun-Loong Tham
Journal: BMJ (2020)

This axial view of an abdominal and pelvic computed tomogram shows warfarin induced left psoas (black arrow) and retroperitoneal (white arrow) haematomas (fig 1).



Fig 1 

The patient—a man in his 50s with no previous trauma—had worsening left posterior …
First-in-Human Phase I Dose Escalation and Expansion Study Evaluating the Fc Optimized FLT3 Antibody Flysyn in Acute Myeloid Leukemia Patients with Minimal Residual Disease
Authors: Jonas S. Heitmann, Daniela Dörfel, Sabine Kayser, Michael Heuser, Felicitas Thol, Silke Kapp-Schwoerer, Wolfgang Bethge, Ludger Grosse-Hovest, Martin Steiner, Melanie Märklin, Juliane S. Walz, Richard F. Schlenk, Gundram Jung, Helmut R. Salih
Journal: Blood (2020)
Challenges in Diagnosis and Treatment of Systemic Amyloidosis: 10 Years of Experience in a Public Brazilian University Center
Authors: Roberta Shcolnik Szor, Fabio Fernandes, Fernanda S Seguro, Angelina M Lino, Lecticia B Jorge, Leonardo O Mendonça, Valkercyo A Feitosa, Jussara B Castelli, Eduardo M Rego, Mayara Jacomassi, Lucas BO Alves, Gracia Martinez, Vanderson Rocha
Journal: Blood (2020)
Abstract 341: Protective Role of Myeloid Cell-specific Erbb3 Signaling After Transverse Aortic Constriction
Authors: Haifeng Yin, Amanda J Lessard, Joanne T deKay, Yodit Herrmann, Michael P Robich, Douglas B Sawyer, Sergey Ryzhov
Journal: Circulation Research (2020)

Introduction: Understanding molecular mechanisms underlying cardiac hypertrophy is crucial for protecting against cardiac remodeling, or slowing down its destined pathway to heart failure. Recent studies revealed that myeloid cells play an important role in the regulation of hypertrophic remodeling. Neuregulin-1 (NRG-1) has been shown to play an essential role in the regulation of tissue-protective and pro-survival processes in response to tissue injury in the cardiovascular system. We find that NRG-1 receptors, including ErbB2 and ErbB3, are expressed and functionally active in cardiac myeloid cells. We hypothesized that the NRG-1/ErbB3 signaling in myeloid cells plays a protective role in cardiac hypertrophic remodeling. To test this hypothesis, we examined the effect of Erbb3 gene ablation in mouse myeloid cells on cardiac hypertrophic remodeling induced by pressure overload. Methods and results: Myeloid-specific ErbB3-deficient mice (ErbB3 MyeKO ) were generated by crossing Erbb3 -floxed mice with LysM-Cre transgenic mice. Cardiac hypertrophic remodeling was established in mice by transverse aortic constriction (TAC). Five days after TAC, survival was dramatically reduced in male ErbB3 MyeKO mice (20% vs. 90%, p=0.021 , MyeKO vs. MyeWT). The post-mortem examination of lung weight to body weight ratio suggested that acute pulmonary edema was developed in ERBB3 MyeKO mice. In order to determine the cellular and molecular mechanisms involved in the increased mortality in ErbB3 MyeKO males, cardiac cell populations were examined at day 3 post-TAC using flow cytometry. A significant accumulation of myeloid cells was found in control but not in ErbB3 MyeKO males. This was accompanied by increased proliferation of Sca-1 positive non-immune cells (endothelial cells and cardiac progenitors) in control but not ErbB3 MyeKO males. An antibody-based protein array analysis revealed that IGF-1 expression was significantly downregulated only in ErbB3 MyeKO males after TAC. Conclusion: Our data highlight the important role of myeloid cell-specific ErbB3 signaling in the accumulation of myeloid cells that contributes to the development of compensatory hypertrophy and the prevention of acute heart failure in male mice.
Group II Introns Generate Functional Chimeric Relaxase Enzymes with Modified Specificities through Exon Shuffling at Both the RNA and DNA Level
Authors: Félix LaRoche-Johnston, Rafia Bosan, Benoit Cousineau
Journal: Molecular Biology and Evolution (2020)

Group II introns are large self-splicing RNA enzymes with a broad but somewhat irregular phylogenetic distribution. These ancient retromobile elements are the proposed ancestors of approximately half the human genome, including the abundant spliceosomal introns and non-long terminal repeat retrotransposons. In contrast to their eukaryotic derivatives, bacterial group II introns have largely been considered as harmful selfish mobile retroelements that parasitize the genome of their host. As a challenge to this view, we recently uncovered a new intergenic trans-splicing pathway that generates an assortment of mRNA chimeras. The ability of group II introns to combine disparate mRNA fragments was proposed to increase the genetic diversity of the bacterial host by shuffling coding sequences. Here, we show that the Ll.LtrB and Ef.PcfG group II introns from Lactococcus lactis and Enterococcus faecalis respectively can both use the intergenic trans-splicing pathway to catalyze the formation of chimeric relaxase mRNAs and functional proteins. We demonstrated that some of these compound relaxase enzymes yield gain-of-function phenotypes, being significantly more efficient than their precursor wild-type enzymes at supporting bacterial conjugation. We also found that relaxase enzymes with shuffled functional domains are produced in biologically relevant settings under natural expression levels. Finally, we uncovered examples of lactococcal chimeric relaxase genes with junctions exactly at the intron insertion site. Overall, our work demonstrates that the genetic diversity generated by group II introns, at the RNA level by intergenic trans-splicing and at the DNA level by recombination, can yield new functional enzymes with shuffled exons, which can lead to gain-of-function phenotypes.
Response of Patients with Transfusion-Dependent β-Thalassemia (TDT) to Betibeglogene Autotemcel (beti-cel; LentiGlobin for β-Thalassemia) Gene Therapy Based on <i>HBB</i> Genotype and Disease Genetic Modifiers
Authors: Mark C. Walters, David H.K. Chui, John J Farrell, Ashutosh Lal, Franco Locatelli, Janet L. Kwiatkowski, John B. Porter, Martin G. Sauer, Isabelle Thuret, Suradej Hongeng, Andreas E. Kulozik, Adrian J. Thrasher, Evangelia Yannaki, Julia Yang, Dustin Whitney, Alexandria Petrusich, Richard A. Colvin, Alexis A. Thompson
Journal: Blood (2020)
Trends in Inpatient Chemotherapy Hospitalizations, Cost and Mortality for Patients with Hematological Malignancies: Insights from the National Inpatient Sample
Authors: Ghulam Rehman Mohyuddin, Kellen Gil, Brian McClune, Nausheen Ahmed, Al-Ola Abdallah, Siddhartha Ganguly, Joseph P. McGuirk, Leyla Shune, Saqib Abbasi
Journal: Blood (2020)
Gene Expression and Epigenetic Analysis in Relapsed/Refractory Diffuse Large B Cell Lymphoma Provides Insights into Evolution of Treatment Resistance to R-CHOP
Authors: Manishkumar S. Patel, Ellen K. Kendall, Sarah Ondrejka, Agrima Mian, Yazeed Sawalha, Bo Hu, Eric D. Hsi, Brian T. Hill, Neetu Gupta
Journal: Blood (2020)
Abstract PO-015: Ex-vivo stimulation of chimeric antigen receptor T cell pre-infusion products predicts clinical response in pediatric B cell acute lymphoblastic leukemia
Authors: Steven Woodhouse, Zhiliang Bai, Rong Fan, Pablo G. Camara, J. Joseph Melenhorst
Journal: Cancer Research (2020)

Abstract CAR T cell therapy has shown remarkable efficacy against refractory hematological malignancies, frequently leading to complete remission. However, some patients do not respond to the therapy, and many patients who do show an initial response eventually relapse. The factors determining these diverse patient responses remain to be understood. We sought to assess whether the intrinsic molecular characteristics of CAR T pre-infusion products could be predictive of clinical outcomes, reasoning that differences in T cell cellular states and subtype proportions determine response. To investigate the heterogeneity and temporal dynamics of CD19-targeted CAR T cells at a molecular level and the relationship to clinical variables in pediatric B cell acute lymphoblastic leukemia, we profiled by single-cell RNA-seq patient-derived pre-infusion CAR T cells before and after stimulation ex-vivo by antigen presenting cells. Computational analysis of data from 12 pediatric patients with chemotherapy-resistant or refractory disease revealed distinct populations of T cells associated with clinical response. We found that CAR T cells from patients with a favorable response had an increased potential to transition to an active, cytokine-expressing state upon stimulation with CD19, and that responding patients were particularly associated with a robust mixed CD4/CD8 Type 1 helper-like response. Conversely, we found that patients who do not did not respond to CAR T therapy had a higher proportion of non-cytokine expressing CD8 cytotoxic T cells upon stimulation. These results demonstrate the potential of single-cell gene expression profiling coupled with computational analysis to uncover the molecular factors determining response to immunotherapy. Citation Format: Steven Woodhouse, Zhiliang Bai, Rong Fan, Pablo G. Camara, J. Joseph Melenhorst. Ex-vivo stimulation of chimeric antigen receptor T cell pre-infusion products predicts clinical response in pediatric B cell acute lymphoblastic leukemia [abstract]. In: Proceedings of the AACR Virtual Special Conference on Tumor Heterogeneity: From Single Cells to Clinical Impact; 2020 Sep 17-18. Philadelphia (PA): AACR; Cancer Res 2020;80(21 Suppl):Abstract nr PO-015.
Determination of Relapse Risk By Complement Gene Variants after Eculizumab Discontinuation in Complement-Mediated Thrombotic Microangiopathy: A Retrospective Review
Authors: Aldo A. Acosta-Medina, Ann M. Moyer, Ronald S. Go, Maria Alice V. Willrich, Hassan B. Alkhateeb, Christianne Bourlon De Los Rios, Dong Chen, Fernando C. Fervenza, Nahla M. Heikal, Nelson Leung, Ariela L. Marshall, Cheryl L. Tran, Jeffrey L. Winters, Meera Sridharan
Journal: Blood (2020)
Point-of-care tests for influenza A and B viruses and RSV in emergency departments – indications, impact on patient management and possible gains by syndromic respiratory testing, Capital Region, Denmark, 2018
Authors: Uffe Vest Schneider, Mona Katrine Alberthe Holm, Didi Bang, Randi Føns Petersen, Shila Mortensen, Ramona Trebbien, Jan Gorm Lisby
Journal: Eurosurveillance (2020)

Background Point-of-care tests (POCT) for influenza A and B viruses and respiratory syncytial virus (RSV) were implemented in emergency departments of all hospitals in the Capital Region of Denmark in 2018. Aim To establish whether POC testing for influenza viruses or RSV is based on a valid respiratory symptom indication, whether changes in patient management based on a positive result are safe and whether syndromic POC testing may benefit patients with influenza or RSV. Methods Samples from 180 children (&lt; 18 years) and 375 adults tested using POCT between February and July 2018 were retested for 26 respiratory pathogens. Diagnosis, indication for POC testing, hospitalisation time, antimicrobial therapy and readmission or death within one month of testing were obtained from patient records. Results A valid indication for POC testing was established in 168 (93.3%) of children and 334 (89.1%) of adults. A positive POCT result significantly reduced antibiotic prescription and median hospitalisation time by 44.3 hours for adults and 14.2 hours for children, and significantly increased antiviral treatment in adults. Risk of readmission or death was not significantly altered by a positive result. Testing for 26 respiratory pathogens established that risk of coinfection is lower with increasing age and that POCT for adults should be restricted to the influenza and RSV season. Conclusion Positive POCT resulted in changed patient management for both children and adults, and was deemed safe. POCT for additional pathogens may be beneficial in children below 5 years of age and outside the influenza and RSV season.
Use of Apremilast in Patients With Psoriatic Arthritis During the COVID‐19 Pandemic: Comment on the Article by Mikuls et al
Authors: Pankaj Bansal, Amandeep Goyal, Austin Cusick, Fawad Aslam
Journal: Arthritis & Rheumatology (2020)

Our approach has limitations.The gender-matching package was unable to match the majority of authors and was limited to Social Security Administration data from 1970 for names.The searches only retrieved articles that were indexed for Medline and utilized MeSH terms, which may be inconsistently applied to publications.Relevant variables assessed by Bagga and colleagues, such as industry funding and article type, could not be ascertained.These limitations notwithstanding, our data complement Figure 1.Women as first, middle, and senior authors of non-pediatric rheumatology articles (A), pediatric rheumatology publications (B), and articles reporting rheumatology randomized controlled trials (RCTs) (C).Data are presented as 3-year rolling means.Percentage labels indicate initial (2005) and final (2020) values.the findings of Bagga et al, highlighting notable gender disparities with respect to senior authorship, authorship of articles reporting RCTs, and authorship of studies involving pediatric patients.
Genomic and Transcriptomic Characterization of Adult and Pediatric Relapsed Acute Myeloid Leukemia Reveals Novel Therapeutic Targets
Authors: Svea Stratmann, Sara A Yones, Jitong Sun, Aron Skaftason, Markus Mayrhofer, Nina Norgren, Morten Krogh Herlin, Anna Eriksson, Martin Hoglund, Josefine Palle, Kirsi Jahnukainen, Katja Pokrovskaja Tamm, Lucia Cavelier, Linda Holmfeldt
Journal: Blood (2020)
Intravenous nanoparticle vaccination generates stem-like TCF1+ neoantigen-specific CD8+ T cells
Authors: Faezzah Baharom, Ramiro A. Ramirez-Valdez, Kennedy K. S. Tobin, Hidehiro Yamane, Charles-Antoine Dutertre, Ahad Khalilnezhad, Glennys V. Reynoso, Vincent L. Coble, Geoffrey M. Lynn, Matthew P. Mulè, Andrew J. Martins, John P. Finnigan, Xiao Meng Zhang, Jessica A. Hamerman, Nina Bhardwaj, John S. Tsang, Heather D. Hickman, Florent Ginhoux, Andrew S. Ishizuka, Robert A. Seder
Journal: Nature Immunology (2020)
Abstract 299: Cardiac Fibroblast-derived FGF21 is a Novel Therapeutic Target for Cardiac Pathological Remodeling
Authors: Nozomi Furukawa, Norimichi Koitabashi, Hiroki Matsui, Tomoyuki Yokoyama, Masahiko KURABAYASHI
Journal: Circulation Research (2020)

Background: Fibroblast Growth factor 21(FGF21) is an endocrine factor, secreted mainly by the liver, that exerts metabolic actions. It has been shown that FGF21 has an anti-hypertrophic action for cardiac hypertrophy. We previously reported that murine cardiac FGF21 expression was upregulated by pressure-overload. The objective of this study was to clarify the role of cardiac fibroblast-derived FGF21 in cardiac pathological remodeling. Methods and Results: We generated fibroblast-specific/tamoxifen-inducible FGF21 knockout mice crossing by FGF21flox mice and Col1a2-CreERT mice (FB-FGF21KO). FGF21flox control mice and KO mice were induced cardiac hypertrophy by transverse aortic constriction (TAC). After 3-weeks TAC surgery, we evaluated the cardiac function by echocardiography or conductance catheter. TAC-mediated cardiac hypertrophy or impairment of systolic function were exacerbated in FB-FGF21KO mice. Conductance catheter showed that Tau, diastolic relaxation marker, was exacerbated in FB-FGF21KO mice with TAC. FB-FGF21KO heart with TAC showed significant upregulation in profibrotic genes and down-regulation in Ca2+ ATPase in comparison with control TAC heart. Cardiac Sirt1, which plays a central role in energy metabolism or oxidative stress, increased by TAC was significantly attenuated in FB-FGF21KO heart. Conclusion: Deletion of FGF21 in cardiac fibroblasts exacerbates cardiac dysfunction in response to pressure overload suggesting that cardiac fibroblasts regulates pathological remodeling via FGF21 regulation.
Bi-allelic pathogenic variations in DNAJB11 cause Ivemark II syndrome, a renal-hepatic-pancreatic dysplasia
Authors: Penelope Jordan, Christelle Arrondel, Bettina Bessières, Aude Tessier, Tania Attié-Bitach, Sarah Guterman, Vincent Morinière, Corinne Antignac, Sophie Saunier, Marie-Claire Gubler, Laurence Heidet
Journal: Kidney International (2020)