<i>Fusobacterium</i>-Positive and Streptococcal-Positive Pharyngitis
Authors: James R. Uhl, Daniel R. Gustafson, Stefanea L. Rucinski, Robin Patel
Journal: Annals of Internal Medicine (2015)

Letters16 June 2015Fusobacterium-Positive and Streptococcal-Positive PharyngitisJames R. Uhl, MS, Daniel R. Gustafson, BS, Stefanea L. Rucinski, MT (ASCP), and Robin Patel, MDJames R. Uhl, MSFrom Mayo Clinic, Rochester, Minnesota.Search for more papers by this author, Daniel R. Gustafson, BSFrom Mayo Clinic, Rochester, Minnesota.Search for more papers by this author, Stefanea L. Rucinski, MT (ASCP)From Mayo Clinic, Rochester, Minnesota.Search for more papers by this author, and Robin Patel, MDFrom Mayo Clinic, Rochester, Minnesota.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/L15-5099-2 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:We developed a real-time polymerase chain reaction (PCR) assay to detect F. necrophorum in throat swabs; assay evaluation yielded findings that support and expand those of Centor and colleagues (1). Between November 2010 and January 2011, a total of 239 swabs submitted for Streptococcus pyogenes PCR testing (2) was evaluated for F. necrophorum using culture and PCR targeting the phosphotransferase system I gene using fluorescence resonance energy transfer hybridization probes. For PCR, swabs were cut into 50 µL of 0.1-mm glass beads and 600 µL of Tris buffer solution and processed using an Eppendorf ThermoMixer (Eppendorf AG) for ...References1. Centor RM, Atkinson TP, Ratliff AE, Xiao L, Crabb DM, Estrada CA, et al. The clinical presentation of Fusobacterium-positive and streptococcal-positive pharyngitis in a university health clinic: a cross-sectional study. Ann Intern Med. 2015;162:241-7. [PMID: 25686164] doi:10.7326/M14-1305 LinkGoogle Scholar2. Uhl JR, Adamson SC, Vetter EA, Schleck CD, Harmsen WS, Iverson LK, et al. Comparison of LightCycler PCR, rapid antigen immunoassay, and culture for detection of group A streptococci from throat swabs. J Clin Microbiol. 2003;41:242-9. [PMID: 12517855] CrossrefMedlineGoogle Scholar3. Eaton C, Swindells J. The significance and epidemiology of Fusobacterium necrophorum in sore throats [Letter]. J Infect. 2014;69:194-6. [PMID: 24642207] doi:10.1016/j.jinf.2014.03.005 CrossrefMedlineGoogle Scholar4. Hedin K, Bieber L, Lindh M, Sundqvist M. The aetiology of pharyngotonsillitis in adolescents and adults—Fusobacterium necrophorum is commonly found. Clin Microbiol Infect. 2015;21:263. [PMID: 25658556] doi:10.1016/j.cmi.2014.08.020 CrossrefMedlineGoogle Scholar5. Jensen A, Hansen TM, Bank S, Kristensen LH, Prag J. Fusobacterium necrophorum tonsillitis: an important cause of tonsillitis in adolescents and young adults. Clin Microbiol Infect. 2015;21:266. [PMID: 25658551] doi:10.1016/j.cmi.2014.09.020 CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAuthors: James R. Uhl, MS; Daniel R. Gustafson, BS; Stefanea L. Rucinski, MT (ASCP); Robin Patel, MDAffiliations: From Mayo Clinic, Rochester, Minnesota.Disclosures: Disclosures can be viewed at www.acponline.org/authors/icmje/ConflictOfInterestForms.do?msNum=L15-0189. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoThe Clinical Presentation of Fusobacterium-Positive and Streptococcal-Positive Pharyngitis in a University Health Clinic Robert M. Centor , T. Prescott Atkinson , Amy E. Ratliff , Li Xiao , Donna M. Crabb , Carlos A. Estrada , Michael B. Faircloth , Lisa Oestreich , Jeremy Hatchett , Walid Khalife , and Ken B. Waites The Clinical Presentation of Fusobacterium-Positive and Streptococcal-Positive Pharyngitis in a University Health Clinic Robert M. Centor , T. Prescott Atkinson , Amy E. Ratliff , Li Xiao , Donna M. Crabb , Carlos A. Estrada , Michael B. Faircloth , Lisa Oestreich , Jeremy Hatchett , Walid Khalife , and Ken B. Waites Fusobacterium-Positive and Streptococcal-Positive Pharyngitis Jan Matthys , Marc De Meyere , and An De Sutter Fusobacterium-Positive and Streptococcal-Positive Pharyngitis Robert M. Centor , T. Prescott Atkinson , and Ken B. Waites Fusobacterium-Positive and Streptococcal-Positive Pharyngitis Robert M. Centor , T. Prescott Atkinson , and Ken B. Waites Fusobacterium-Positive and Streptococcal-Positive Pharyngitis Jan Matthys , Marc De Meyere , and An De Sutter Metrics Cited byPoint-Counterpoint: A Nucleic Acid Amplification Test for Streptococcus pyogenes Should Replace Antigen Detection and Culture for Detection of Bacterial Pharyngitis 16 June 2015Volume 162, Issue 12Page: 876-877KeywordsAdolescentsDisclosurePeritonsillar abscessesPharyngitisPharynxPolymerase chain reactionRibosomal RNAStreptococcus pyogenesVancomycinYoung adults ePublished: 16 June 2015 Issue Published: 16 June 2015 Copyright & PermissionsCopyright © 2015 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...