tbuckley/cabot-search huggingface dataset:
Regulation of cytoplasmic and nuclear PTEN levels by human papillomavirus (HPV) type 16 E6 and E7 oncogenes in HPV16+ oropharyngeal cancer cell line
Authors: A. Psyrri, C. Sasaki, B. A. Burtness, T. Rampias
Journal: Journal of Clinical Oncology (2008)

6034 Background: We have previously shown that repression of HPV type 16 E6 and E7 genes using short hairpin RNAs to HPV16+ oropharyngeal cancer cells results in restoration of cellular p53 and pRb tumor suppressor pathways. PTEN is a cellular tumor suppressor protein that functions as a plasma membrane lipid phosphatase antagonizing the phosphoinositide 3-kinase/AKT cell survival pathway. PTEN is also found in cell nuclei maintaining the chromosomal stability. We sought to investigate the role of HPV E6 and E7 oncogenes in the regulation of PTEN tumor suppressor protein levels in HPV16+ oropharyngeal cancer cells. Methods: Short hairpin RNAs targeting HPV type 16 E6 and E7 genes were delivered by retrovirus infection to HPV16+ oropharyngeal cancer cell lines 147T and 090. E6 and E7 mRNA downregulation was assessed by quantitative reverse transcription polymerase chain reaction and western blotting for p53 and Rb. The protein levels of AKT, pAKT and PTEN were analyzed before and after silencing in 147T and 090 oropharygeal cell lines by western blotting. Quantitative real time RT-PCR analysis was also used to detect changes to mRNA expression of AKT and PTEN after E6/E7 repression. Results: The protein levels of cytoplasmic and nuclear PTEN were significantly upregulated after E6/E7 gene silencing. The upregulation of PTEN protein after silencing was not associated with a change in the phosphorylated status of AKT. Quantitative real time RT-PCR analysis showed that PTEN mRNA levels were not significantly upregulated after E6/E7 repression. Conclusions: We provide evidence that the principal HPV16 oncoproteins (E6 and E7) are involved in downregulation of cytoplasmic and nuclear PTEN protein levels in HPV16+ oropharygeal cancer cell lines. PTEN upregulation after E6/E7 silencing occurs at the protein and not at the mRNA level. Thus, it is possible that PTEN protein stabilization after E6/E7 repression is associated with cellular restoration of p53 protein. The lack of correlation between PTEN downregulation and AKT activation is consistent with data showing that PTEN tumorigenesis also occurs via AKT-independent mechanisms. No significant financial relationships to disclose.
Comparative chemopreventive efficacy of Naproxen and NO-Naproxen in rodent models of colon and bladder cancer.
Authors: Vernon Steele, Chinthalapally Rao, Yuting Zhang, Jagan Patlolla, Clinton Grubbs, Ronald Lubet
Journal: Cancer Research (2008)

AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA

3054 

Nonsteroidal antiinflammatory drugs have proven to be very efficacious to prevent colon cancer in humans and in animal models. They are also very effective chemopreventive agents in experimental animal models of bladder and skin cancer. However the major side effect of such agents is gastrointestinal ulceration and bleeding. Recently it was found that adding a nitrous oxide (NO) group to NSAIDs alleviated this GI toxicity side effect through the protective activity of NO, yet still inhibited cyclooxygenase activity. Our studies were designed to compare the chemopreventive effects of NO-naproxen with the parent compound Naproxen administered in equimolar concentrations in the diet. In the AOM-induced rat colon cancer model Naproxen was administered at 200 and 400 ppm in the diet reduced mean aberrant crypt foci (ACF) in the colon by about 45 and 60% respectively. NO-Naproxen was likewise administered at 300 and 600 ppm by diet and reduced total ACF by 20 and 40% respectively. Importantly, Naproxen and NO-Naproxen at the higher doses reduced multi-crypt foci (4 or more) in colon by 72% and 50%, respectively. In the OH-BBN rat bladder cancer model NO-Naproxen and Naproxen were given at 550 ppm and 400 ppm in the diet respectively. After 6 months the NO-Naproxen group had a 60% decrease in palpable bladder tumors, while the Naproxen group showed a 53% decrease in incidence. These data show that both Naproxen and NO-Naproxen are effective agents against colon and bladder cancers and show similar efficacies. The decrease in gastrointestinal mucosal toxicity seen with the NO-Naproxen would give this agent a distinct advantage in clinical trials for colon or bladder cancer prevention, while the efficacy should be similar to the parent compound. Supported by NCI -CN53300 and CN43301.
No blood pressure lowering agents prolong survival in diabetes and kidney disease, review shows
Authors: S. Mayor
Journal: BMJ (2015)

No blood pressure lowering agents used either alone or in combination prolong survival in adults with diabetes and kidney disease, a meta-analysis published in the Lancet has found.1

Researchers analysed 157 studies comparing blood pressure lowering agents in a total of 43 256 patients with diabetic kidney disease, mostly type 2 diabetes and chronic kidney disease. The trials were identified by searching electronic databases systematically for trials published up to January 2014 that compared several classes of blood pressure lowering drugs, used alone or …
Proapoptotic Kinase Mst1 Restricts Myocyte Hypertrophy by Managing Unfolded Protein Responses
Authors: Soichiro Usui
Journal: Circulation (2007)

Abstract 288 Soichiro Usui, Chull Hong, Shumin Gao, Junichi Sasoshima, UMDNJ New Jersey Medical School, Newark, NJ Soichiro Usui, 2007 Finalist and Presenting Author
Abstract 19973: Dynamic Thallium-201 SPECT/CT Provides Quantitative Index of Myocardial Flow and Viability: Validation in Porcine Post-Myocardial Infarction Model
Authors: Stephanie L Thorn, Mitchel R Stacy, Alda Bregasi, Jin C Paeng, Vahid A Tavakoli, James O Winters, Chris Hawley, Yangfeng Tang, James Bennett, R. G Wells, Chi Liu, Ran Klein, Albert J Sinusas
Journal: Circulation (2014)

Introduction: Thallium-201 (Tl-201) SPECT imaging is routinely used to evaluate relative myocardial perfusion and/or viability. In this study, we have evaluated Tl-201 kinetics in a porcine model of myocardial infarction (MI) with dynamic imaging on a hybrid SPECT/CT system. Methods: Tl-201 SPECT and contrast CT imaging were performed on hybrid SPECT/64-slice CT (GE Discovery NM/CT 570c) in Yorkshire pigs (n=5) one week following a 90 min balloon occlusion of the LAD below the first diagonal branch. Tl-201 (83-158 MBq) was injected after the initiation of a 900s list-mode acquisition. The list-mode data was reconstructed with MLEM using 30 iterations creating 23 frames (12 x 10s; 6 x 30s; 5 x 120s). Reconstructed dynamic SPECT data was analyzed using FlowquantTM semi-automated analysis software with a 1-compartment model. K1 (index of flow) and k2 (index of viability) were assessed within a 17-segment model of the left ventricle. Four segments within the central infarcted LAD territory were compared to six segments of the contralateral inferolateral wall. The same segments were analyzed for wall thickening from ECG-gated contrast CT scans using centerline approach (GE AW Cardiac Function Xpress). Results: K1 was significantly decreased in the MI vs contralateral normal regions (0.52 ± 0.17 vs 0.61 ± 0.16 ml/min/g, p&lt;0.02). K2 an index of tissue viability was significantly increased in the MI region (0.42 ± 0.19 vs 0.32 ± 0.12 /min, p&lt;0.04), reflecting accelerated Tl-201 washout in MI. Wall thickening in the same MI region was significantly decreased (0.33 ± 1.5 mm vs 3.3 ± 1.9 mm, p&lt;0.005). Conclusions: Dynamic Tl-201 SPECT/CT imaging provides a quantitative index of both myocardial flow and viability using a 1-compartment model. This preliminary pre-clinical demonstrates the potential for dynamic Tl-201 SPECT imaging to quantify myocardial viability early post-MI.
Early versus late partial sleep deprivation in patients with premenstrual dysphoric disorder and normal comparison subjects
Authors: B L Parry, H Cover, N Mostofi, B LeVeau, P A Sependa, A Resnick, J C Gillin
Journal: American Journal of Psychiatry (1995)

The aim of this study was to compare the clinical effects of early-night and late-night partial sleep deprivation in patients with premenstrual dysphoric disorder and normal comparison subjects.In the premenstrual phase of two menstrual cycles, 23 subjects with DSM-IV premenstrual dysphoric disorder and 18 normal comparison subjects underwent a randomized crossover trial of 1) early-night sleep deprivation, in which subjects slept from 3:00 a.m. to 7:00 a.m., followed by a night of recovery sleep (11:00 p.m. to 7:00 a.m.), and 2) late-night sleep deprivation, in which subjects slept from 9:00 p.m. to 1:00 a.m., followed by a night of recovery sleep.For the subjects with premenstrual dysphoric disorder, in both partial sleep deprivation conditions the Hamilton and Beck depression ratings were significantly lower after recovery sleep than at baseline. Ratings on the day after early or late partial sleep deprivation tended to be lower than at baseline but were not statistically different. The normal comparison subjects showed no clinically important mood changes. A factor analytic approach used with the Hamilton depression scores showed that depressive retardation symptoms were the most responsive to sleep deprivation in the premenstrual dysphoric disorder subjects.These results are consistent with the reported efficacy of sleep deprivation for major depressive disorder. However, the premenstrual dysphoric disorder subjects improved after the recovery sleep rather than directly after partial sleep deprivation. That late-night sleep deprivation did not have greater benefit than did the hypothesized sham treatment, early-night sleep deprivation, also suggests that placebo effects cannot be ruled out.
Tumor size, age and stage in patient detected breast cancer
Authors: H. G. Kaplan, J. A. Malmgren, M. Atwood
Journal: Journal of Clinical Oncology (2005)

716 Background: At our institution up to 45% of all breast cancer (BC) cases and 90% of <40 year old BC cases are patient detected (PD) with a higher rate of mortality compared to mammography detected breast cancer (MGD). Our objective is to identify explanatory factors for the increased mortality. Methods: A retrospective cohort study design was used to analyze data on all women age 21–94 diagnosed with primary invasive breast cancer from a community based institution cancer registry of patients diagnosed between 1989–1999 with follow up to 2003/2004 (n=2327). Initial breast cancer detection method abstracted from patient charts were mammography, physician exam or patient detection. Our intermediate endpoints were age, tumor size, detection method and mammography results from the patient record. Our final endpoint was disease specific mortality obtained from registrar follow up and supplemented by SEER registry data. Results: Tumor size was significantly larger in every age group and TNM stage I-III for patient detected breast cancer (table). In a Cox proportional hazards model corrected for age and stage, PD cases had an increased disease specific mortality risk compared to MGD detected cases (OR = 1.46, 95% CI: 1.06 - 2.03). Subsequent to diagnosis, 75% of PD cases had mammograms with 28% of their tumors not seen on the mammograms.Tumors seen v. not seen on mammography in PD cases did not differ significantly by patient age or tumor size. Conclusions: At every age and in stage I-III, PD breast cancer had consistently larger tumors yet a substantial number were not detectable by mammography. Patient detected breast cancer may represent a biologically distinct subset of patients at increased mortality risk. Further characterization of the PD breast cancer population is required to identify effective detection and treatment modalities. Table ANOVA mean tumor size by detection method (n=2327) Patient (n=1135) Mean (95% CI) Mammography (n=1192) Mean (95% CI) Total 2.43 cm (range .10–18.50) 1.39 cm (range .10-.90) F Statistic = 178.60, p=.000 Age 21–39 2.51 (2.32, 2.70) 1.32 (.77, 1.87) 40–49 2.33 (2.19, 2.47) 1.57 (1.39, 1.76) 50–59 2.39 (2.22, 2.56) 1.43 (1.28, 1.57) 60–69 2.48 (2.29, 2.67) 1.33 (1.18, 1.48) 70–94 2.53 (2.33, 2.73) 1.30 (1.16, 1.45) F statistic: 40.93, p=.000 TNM stage I 1.34 (1.26, 1.43) 1.09 (1.03, 1.15) IIA 2.19 (2.10, 2.28) 1.75 (1.64, 1.86) IIB 3.27 (3.16, 3.38) 2.81 (2.60, 3.02) IIIA 6.22 (6.01, 6.43) 5.28 (4.77, 5.78) IIIB 4.85 (4.43, 5.28) 2.70 (1.47, 3.93) IV 3.68 (3.24, 4.11) 3.56 (2.95, 4.18) F statistic: 335.10, p=.000 No significant financial relationships to disclose.
Cardiopulmonary Benefits of Reducing Indoor Particles of Outdoor Origin
Authors: Renjie Chen, Ang Zhao, Honglei Chen, Zhuohui Zhao, Jing Cai, Cuicui Wang, Changyuan Yang, Huichu Li, Xiaohui Xu, Sandie Ha, Tiantian Li, Haidong Kan
Journal: Journal of the American College of Cardiology (2015)
Radionuclide Salivagram and Gastroesophageal Reflux Scintigraphy in Pediatric Patients
Authors: Jigang Yang, Ion Codreanu, Sabah Servaes, Hongming Zhuang
Journal: Clinical Nuclear Medicine (2015)

Both gastroesophageal reflux (GER) scintigraphy and radionuclide salivagram are commonly used in the detection of pulmonary aspiration in pediatric patients. This investigation is to compare the diagnostic value of these 2 imaging methods.This retrospective study included 4186 pediatric patients (aged 1 week to 16 years; mean age, 28 months) who underwent a GER scintigraphy and/or radionuclide salivagram. Detection rate of pulmonary aspiration by the 2 imaging techniques was compared.The detection rate for pulmonary aspiration in patients undergoing both procedures was 1.9% (5 of 266) for GER scintigraphy and 22.2% (59 of 266) for radionuclide salivagram. Fifty-six of 59 patients with proven aspiration on radionuclide salivagram demonstrated no such findings on GER scintigraphy, whereas 2 of 5 patients with proven aspiration on GER scintigraphy demonstrated no such findings on radionuclide salivagram. In patients who underwent only 1 procedure (either GER scintigraphy or salivagram), the detection rate for pulmonary aspiration was 0.4% (15 of 3551) for GER scintigraphy and 20.3% (75 of 369) for radionuclide salivagram.Radionuclide salivagram showed a much higher detection rate for pulmonary aspiration compared with GER scintigraphy. However, this may be related to a significantly higher prevalence of antegrade versus retrograde aspiration in our study population. Our results also suggest that not all episodes of retrograde aspiration can be detected by a radionuclide salivagram, and the requested scan should be tailored to the type of suspected aspiration.
Fortnightly Review Biology and clinical relevance of nitric oxide
Authors: P Vallance, J Collier
Journal: BMJ (1994)

Ten years ago the idea that nitric oxide might be a biological mediator would have seemed far fetched.Clinicians might have recalled from schooldays that nitric oxide is a clear, colourless gas which rapidly turns brown on contact with air, anaesthetists might have remembered the series of disasters related to inadvertent contamination of cylinders of nitrous oxide with nitric oxide, the environmentally aware would have been familiar with the gas as a product of car exhaust and cigarette smoke and as a pollutant, and for others it would have conjured up images of primordial swamps and electrochemical storms.However, in 1987 it was shown that mammalian cells synthesise nitric oxide, a year later it was suggested that cells communicate with each other by the synthesis of nitric oxide from the amino acid arginine,' and in 1993 there were over 1000 publications on the biology of nitric oxide.This improbable biological mediator has been implicated in the pathogenesis of diseases ranging from hypertension to septic shock and dementia.'
Abstract 4855: Influence of Left Ventricular End Diastolic Diameter Index on Clinical Outcomes with Cardiac Resynchronization: Insights from the COMPANION Study
Authors: Teresa De Marco, Byron Lee, JoAnn Lindenfeld, Leslie A Saxon, John Boehmer, Mintu Turakhia, Jill Leigh, Patrick Yong, Colleen Curran, Arthur M Feldman, Michael R Bristow
Journal: Circulation (2008)

Background: Although cardiac resynchronization therapy (CRT) has been shown to slow the progression of heart failure (HF), it has not been well described whether the baseline severity of ventricular remodeling influences clinical outcomes. Methods: The COMPANION study database was analyzed retrospectively. This study randomized patients (pts) with NYHA Class III/IV HF, wide QRS, and systolic dysfunction to optimal pharmacologic therapy (OPT) alone or to OPT plus CRT either with (CRT-D) or without a defibrillator (CRT-P). For this analysis both CRT arms were combined. Ventricular remodeling was measured as left ventricular end diastolic dimension indexed by body surface area (LVEDDI). Data were stratified around the median value: LVEDDI≥ 35 mm/m 2 and &lt; 35 mm/m 2 . CRT was compared to OPT for each cohort using functional outcomes at six months [exercise capacity, quality of life, functional status, and the Clinical Composite Endpoint] as well as event-driven outcomes [all-cause mortality alone and in combination with cause-specific hospitalization]. Results: Baseline LVEDDI data were available for 1260 pts. Pts in the larger LVEDDI cohort were characterized at baseline with wider QRS, lower EF, and a greater proportion of non-ischemic, female, and NYHA Class IV. Clinical outcomes are summarized below: Conclusion: In the COMPANION study, both cohorts demonstrated significant benefit in functional outcomes with CRT when compared to OPT. However, significant improvement in event-driven outcomes were seen only in the population with larger baseline LVEDDI. A prospective study is needed to confirm that LVEDDI can predict potential for improvement with CRT.
Standard of Care for Small Renal Masses in the 21st Century
Authors: Joshua J. Meeks, Chris M. Gonzalez
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Abstract 156: Pediatric Basic Life Support Self-Training Is Comparable to Instructor-Led Training: A Randomized Manikin Study
Authors: Lone D Vestergaard, Bo Løfgren, Casper Jessen, Christina Børlum, Anne Wolff, Henrik Vendelboe Nielsen, Niels Henrik Vinter Krarup
Journal: Circulation (2011)

Background: Pediatric cardiac arrest carries a poor prognosis. Basic life support improves survival. Previous studies have shown promising results in teaching adult basic life support using self-tr...
Interventional pharmacotherapy
Authors: R. Philipp
Journal: BMJ (2003)

The dramatic increase in the use of percutaneous coronary intervention has been possible because of advances in adjunctive pharmacotherapy, which have greatly improved safety. Percutaneous intervention inevitably causes vessel trauma, with disruption of the endothelium and atheromatous plaque. This activates prothrombotic factors, leading to localised thrombosis; this may impair blood flow, precipitate vessel occlusion, or cause distal embolisation. Coronary stents exacerbate this problem as they are thrombogenic. For these reasons, drug inhibition of thrombus formation during percutaneous coronary intervention is mandatory, although this must be balanced against the risk of bleeding, both systemic and at the access site.​site.



Figure 1

Action of antiplatelet and antithrombotic agents in inhibiting arterial thrombosis
Correlation Of Heparin Confirmatory Test (HCT) With Optical Density (OD) and Serotonin Release Assay (SRA) In The Diagnosis Of Heparin Induced Thrombocytopenia (HIT)
Authors: Ravneet Thind, Danielle Heidemann, Sundara Raman, Philip Kuriakose
Journal: Blood (2013)
Abstract 200: Hsp90 Binding Inhibitors Improve Survival and Attenuate the Inflammatory Insult in a Murine Model of Sepsis-Induced Acute Lung Injury.
Authors: Anuran Chatterjee, Connie Snead, Galina Antonova, Christiana Dimitropoulou, John D Catravas
Journal: Circulation (2006)

Sepsis-induced acute lung injury (ALI) is associated with the activation of numerous pro-inflammatory pathways, key components of which (e.g., NFkB iNOS, Src kinase, STAT3, etc.) are hsp90-dependen...
Abstract 10211: Exaggerated Exercise Blood Pressure Response is Related to Increased Arterial Stiffness, Asymmetric Dimethylarginine and Osteoprotegerin in Essential Hypertensive Subjects
Authors: Kyriakos Dimitriadis, Costas Tsioufis, Athanasios Aggelis, Alexandros Kasiakogias, Michalis Giakoumis, Antigoni Miliou, Andreas Michaelides, Christodoulos Stefanadis
Journal: Circulation (2011)

Introduction: A hypertensive response to exercise (HRE) is associated with high cardiovascular risk, while elevated levels of asymmetric dimethylarginine (ADMA) and osteoprotegerin (OPG) are relate...
American Encyclopedia of Sex
Authors: Adolph Fredrick Niemoeller
Journal: JAMA (1935)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Neonatal Hemolytic Anemia Due to Inherited Harderoporphyria: Clinical Characteristics and Molecular Basis
Authors: J. Lamoril, H. Puy, L. Gouya, R. Rosipal, V. Da Silva, B. Grandchamp, T. Foint, B. Bader-Meunier, J.P. Dommergues, J.C. Deybach, Y. Nordmann
Journal: Blood (1998)