tbuckley/cabot-search huggingface dataset:
Colorectal cancer conversations on Twitter: A content analysis of global perceptions and outreach efforts.
Authors: Alexander L. Chu, Vishal R. Patel, Christopher J. Blanton, Sofia Gereta, Michael Mackert, Nico Nortje, Michael Pignone
Journal: Journal of Clinical Oncology (2022)

35 Background: Screening is highly effective at reducing colorectal cancer (CRC) mortality. Social media is extensively used to communicate about cancer care, yet little is known about the role of these online platforms in promoting CRC screening and early diagnosis. This study tracked Twitter discussions about CRC and characterized participating users to better understand public communication and perceptions of CRC on social media. Methods: Tweets containing references to CRC were collected from January 2020 to April 2021 using Twitter’s Application Programming Interface. Account metadata was used to predict user demographic information and classify users as either organizations, individuals, or influencers. Influencers represent high-impact users, defined by sizeable follower counts or verified Twitter status. Latent Dirichlet Allocation, a natural language processing model, was used to identify observed topics of discussion in the collected tweets. Results: There were 72,229 unique CRC-related tweets by 31,170 users. Tweets reached a daily maximum after Chadwick Boseman, a well-known American actor, died from CRC. Individuals accounted for the majority of users (62.8%); organizations (35.2%); influencers (2%). Influencers made the most median impressions (35,853 impressions). Tweets contained the following topics: bereavement (27.9%), appeals for early detection (19.4%), research (14.0%), National Colorectal Cancer Awareness Month (NCCAM) (13.7%), screening access (12.5%), and risk factors (12.4%). Tweets referencing bereavement had the most user engagement. Links to clinical trial enrollment information were the least shared type of embedded content (0.3%). Conclusions: Discussions about CRC largely focused on bereavement and early detection. Online coverage of NCCAM, personal experiences, and celebrity deaths related to CRC effectively stimulated goal-oriented tweets about early detection. Our findings suggest that Twitter may be a suitable platform for promoting and communicating future public health recommendations about CRC.
Abstract 401: Nuclear Remodeling After Mechanical Circulatory Support
Authors: Jun Luo, Stephen Farris, Deri Helterline, April Stempien-Otero
Journal: Circulation Research (2017)

Rationale: Cardiomyocytes increase DNA content in normal growth and in response to stress in humans by both increases in nuclear number and ploidy. This observation complicates analysis of human cardiomyocyte proliferation as DNA content can increase in the absence of cytokinesis. Proliferation has been reported in cardiomyocytes following LVAD unloading which may represent a reversal of this process. However, cardiac recovery from LVAD is rare. Thus, we sought to analyze changes in cardiomyocyte nuclear characteristics for clues to this paradox. Objective: We used a novel technique to determine changes in nuclear content to test the hypothesis that adult cardiomyocytes can complete cell cycle progression by mitosis after long-term hemodynamic unloading of the failing heart. Methods and Results: The makeup of myocyte nuclear number, ploidy (per cell and per nucleus) and the frequency of cell cycling markers were evaluated by imaging flow cytometry. Hypertrophic hearts from 15 subjects with left ventricular assist device (LVAD) were compared with 8 non-LVAD unloaded hearts. After hemodynamic unloading for 13.7 ± 9.1 months, myocyte nuclear makeup, specifically the average sizes of both cell and individual nuclei, did not significantly change. DNA content per nucleus was significantly decreased (P &lt; 0.01). The frequency of cell cycle markers, i.e. Ki67 and phospho-histon3 (H3P) were not increased after hemodynamic unloading. Conclusions: Our data demonstrate that unloading of failing hearts with mechanical ventricular assist devices does not alter nucleation state of cardiomyocytes. However, unloading is associated with decreased DNA content of nuclei independent of nucleation state within the cell. As these changes were associated with a trend to decreased cell size but not increased cell cycle markers, they may represent a regression of hypertrophic nuclear remodeling.
Influence of Age and Renal Function on Efficacy and Safety of Dabigatran Versus Warfarin for the Treatment of Acute Venous Thromboembolism: A Pooled Analysis of RE-COVER™ and RE-COVER™ II
Authors: Sam Schulman, Henry Eriksson, Ajay Kakkar, Clive Kearon, Sebastian M Schellong, Patrick Mismetti, Martin Feuring, Stefan Hantel, Joerg Kreuzer, Samuel Z. Goldhaber
Journal: Blood (2014)
Relationship between ST segment elevation after acute myocardial infarction and microvascular reperfusion injury evaluated with myocardial contrast echocardiography Paolo Colonna, Abdel Hakeem Seleem, Marco Orr�, Chrisian Cadeddu, Roberta Montisci, Lijun Chen, Enrico Onnis, Luigi Meloni, Sabino Iliceto. Department of Cardiovascular and neurological Sciences, University of Cagliari, Cagliari, Italy
Journal: Journal of the American College of Cardiology (2001)
Obituaries
Journal: JAMA (1977)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Tandem duplication of the MLL gene in myelodysplastic syndrome‐derived overt leukemia with trisomy 11
Authors: Katsuya Yamamoto, Hiroyuki Hamaguchi, Kaoru Nagata, Masaru Kobayashi, Masafumi Taniwaki
Journal: American Journal of Hematology (1997)

Trisomy 11 as a sole chromosomal abnormality is a rare aberration observed in myelodysplastic syndrome (MDS) or acute myeloblastic leukemia (AML). Recently a partial tandem duplication of the MLL gene, located on chromosome band 11q23, has been identified in de novo AML with trisomy 11. We describe a 72-year-old woman suffering from MDS-derived overt leukemia with trisomy 11 and a tandem duplication of the MLL gene. At first the patient was found to have myeloblasts with Auer rods in the peripheral blood and diagnosed as MDS, refractory anemia with excess of blasts in transformation (RAEB-T). After 2 months a picture of overt leukemia (AML; M2) developed as shown by an increased number of myeloblasts. Various chemotherapy regimens had little effect, and she died of disease progression 15 months after admission. During her clinical course, the chromosome analyses consistently showed 47,XX, +11. Southern blot analysis of leukemic blasts on admission and in accelerated phase revealed identical rearranged bands of the MLL gene. Fluorescence in situ hybridization analysis excluded the possibility of masked translocation of the MLL gene to other chromosomes. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis using a forward exon 6 primer and a backward exon 3 primer demonstrated an in-frame fusion of exon 8 with exon 2. Our results indicated that a partial tandem duplication of exons 2-8 of the MLL gene could be observed in MDS-derived overt leukemia as well as de novo AML with trisomy 11. Am. J. Hematol. 55:41-45, 1997. © 1997 Wiley-Liss, Inc.
Extensive hypertensive work-up. In rebuttal to Dr. Melby
Authors: F. A. Finnerty
Journal: JAMA (1975)

ALTHOUGH I readily agree with Dr. Melby that patient noncompliance is the major problem in the long-term treatment of hypertension, the extensive and expensive routine diagnostic work-up that he advocates for every hypertensive patient would aggravate rather than solve the problem. In my experience, both in private practice and in the clinic, the average asymptomatic patient is not willing to spend the time or the money to undergo a battery of tests. Gifford found that less than 6% of 5,000 hypertensive patients examined consecutively at the Cleveland Clinic had secondary hypertension; I would thoroughly agree with Gifford's conclusion that an exhaustive search for the rare types of hypertension in every patient is not warranted. Even if 35% of the hypertensive population has some form of secondary hypertension that can be treated specifically (and most authorities would agree that this estimate is far too high), why must every hypertensive patient be
SARS-CoV-2 infection and COVID-19 vaccination rates in pregnant women in Scotland
Authors: Sarah J. Stock, Jade Carruthers, Clara Calvert, Cheryl Denny, Jack Donaghy, Anna Goulding, Lisa E. M. Hopcroft, Leanne Hopkins, Terry McLaughlin, Jiafeng Pan, Ting Shi, Bob Taylor, Utkarsh Agrawal, Bonnie Auyeung, Srinivasa Vittal Katikireddi, Colin McCowan, Josie Murray, Colin R. Simpson, Chris Robertson, Eleftheria Vasileiou, Aziz Sheikh, Rachael Wood
Journal: Nature Medicine (2022)

Abstract Population-level data on COVID-19 vaccine uptake in pregnancy and SARS-CoV-2 infection outcomes are lacking. We describe COVID-19 vaccine uptake and SARS-CoV-2 infection in pregnant women in Scotland, using whole-population data from a national, prospective cohort. Between the start of a COVID-19 vaccine program in Scotland, on 8 December 2020 and 31 October 2021, 25,917 COVID-19 vaccinations were given to 18,457 pregnant women. Vaccine coverage was substantially lower in pregnant women than in the general female population of 18−44 years; 32.3% of women giving birth in October 2021 had two doses of vaccine compared to 77.4% in all women. The extended perinatal mortality rate for women who gave birth within 28 d of a COVID-19 diagnosis was 22.6 per 1,000 births (95% CI 12.9−38.5; pandemic background rate 5.6 per 1,000 births; 452 out of 80,456; 95% CI 5.1−6.2). Overall, 77.4% (3,833 out of 4,950; 95% CI 76.2−78.6) of SARS-CoV-2 infections, 90.9% (748 out of 823; 95% CI 88.7−92.7) of SARS-CoV-2 associated with hospital admission and 98% (102 out of 104; 95% CI 92.5−99.7) of SARS-CoV-2 associated with critical care admission, as well as all baby deaths, occurred in pregnant women who were unvaccinated at the time of COVID-19 diagnosis. Addressing low vaccine uptake rates in pregnant women is imperative to protect the health of women and babies in the ongoing pandemic.
Detecting Cardiovascular Protein-Protein Interactions by Proximity Proteomics
Authors: Jared S. Kushner, Guoxia Liu, Robyn J. Eisert, Gary A. Bradshaw, Geoffrey S. Pitt, J. Travis Hinson, Marian Kalocsay, Steven O. Marx
Journal: Circulation Research (2022)

Rapidly changing and transient protein-protein interactions regulate dynamic cellular processes in the cardiovascular system. Traditional methods, including affinity purification and mass spectrometry, have revealed many macromolecular complexes in cardiomyocytes and the vasculature. Yet these methods often fail to identify in vivo or transient protein-protein interactions. To capture these interactions in living cells and animals with subsequent mass spectrometry identification, enzyme-catalyzed proximity labeling techniques have been developed in the past decade. Although the application of this methodology to cardiovascular research is still in its infancy, the field is developing rapidly, and the promise is substantial. In this review, we outline important concepts and discuss how proximity proteomics has been applied to study physiological and pathophysiological processes relevant to the cardiovascular system.
Initiation and rapid in-hospital titration of ACE inhibitor is safe in an elderly population with class IV heart failure John M. Cahill, Peter Quigley, Brian Maurer, Ken Mc Donald, Bronagh Travers, Mary Ryder, Mark Ledwidge, ST. VINCENT'S CARDIOMYOPATHY RESEARCH CENTRE, DUBLIN, Ireland, LABORATOIRES SERVIER (IRELAND) LTD., DUBLIN, Ireland
Journal: Journal of the American College of Cardiology (2001)
Comment on Khunti et al. COVID-19, Hyperglycemia, and New-Onset Diabetes. Diabetes Care 2021;44:2645–2655
Authors: Carmine Gazzaruso, Adriana Coppola, Pietro Gallotti, Ileana Terruzzi, Tiziana Montalcini, Livio Luzi
Journal: Diabetes Care (2022)
The D-Health Trial: a randomised controlled trial of the effect of vitamin D on mortality
Authors: Rachel E Neale, Catherine Baxter, Briony Duarte Romero, Donald S A McLeod, Dallas R English, Bruce K Armstrong, Peter R Ebeling, Gunter Hartel, Michael G Kimlin, Rachel O'Connell, Jolieke C van der Pols, Alison J Venn, Penelope M Webb, David C Whiteman, Mary Waterhouse
Journal: The Lancet Diabetes & Endocrinology (2022)
Contents
Authors: P Burra, F Tacke, V Ratziu, S Zeuzem, B Sangro, P Angeli, G Cabibbo, A Singal, V Mallet, L Parlati, A Martinino, J Pereira, C Jimenez, M Sakka, S Bouam, A Retbi, D Krasteva, J.-F Meritet, M Schwarzinger, D Thabut, P Rufat, D Bonnefont-Rousselot, P Sogni, S Pol, E Tsochatzis, J Whitfield, T.-H Schwantes-An, R Darlay, G Aithal, S Atkinson, R Bataller, G Botwin, N Chalasani, H Cordell, A Daly, C Day, F Eyer, T Foroud, D Gleeson, D Goldman, P Haber, J.-M Jacquet, T Liang, S Liangpunsakul, S Masson, P Mathurin, R Moirand, A Mcquillin, C Moreno, M Morgan, S Mueller, B Müllhaupt, L Nagy, P Nahon, B Nalpas, S Naveau, P Perney, M Pirmohamed, H Seitz, M Soyka, F Stickel, A Thompson, M Thursz, E Trépo, T Morgan, D Seth, P Sen, S Qadri, P Luukkonen, O Ragnarsdottir, A Mcglinchey, S Jäntti, A Juuti, J Arola, J Schlezinger, T Webster, M Orešicˇ, H Yki-Järvinen, T Hyötyläinen, H.-S Kim, X Yu, J Kramer, A Thrift, P Richardson, Y.-C Hsu, A Flores, H El-Serag, F Kanwal, R D'ambrosio, E Degasperi, M Anolli, I Fanetti, M Borghi, R Soffredini, M Iavarone, G Tosetti, R Perbellini, A Sangiovanni, V Sypsa, P Lampertico, H Kim, J Nam, H.-C Lee, S Kim, D Sinn, Y Seo, S Park, Y.-S Lim, E Jang, E Yoon, H Kim, S Kim, S Ahn, J.-J Shim, S Jeong, Y Jung, J Sohn, Y Cho, D Jun, G Dalekos, R Idilman, T Berg, M Buti, J Calleja, J Goulis, S Manolakopoulos, H Janssen, M.-J Jang, Y Lee, Y Kim, J.-H Yoon, G Papatheodoridis, J.-H Lee, T Herta, R Kersten, J.-C Chang, L Hubers, S Go, D Tolenaars, C Paulusma, M Nathanson, R Elferink, S Van De Graaf, U Beuers, V Patel, S Lee, M Mcphail, K Da Silva, S Guilly, A Zamalloa, E Witherden, S Støy, G Manakkat Vijay, N Pons, N Galleron, X Huang, S Gencer, M Coen, T Tranah, J Wendon, K Bruce, E Le Chatelier, S Ehrlich, L Edwards, S Shoaie, D Shawcross, D Samuel, R Morikawa, N Nakamoto, T Amiya, P.-S Chu, Y Koda, T Teratani, T Suzuki, Y Kurebayashi, A Ueno, N Taniki, K Miyamoto, A Yamaguchi, S Shiba, T Katayama, K Yoshida, Y Takada, R Ishihara, H Ebinuma, M Sakamoto, T Kanai, S.-T Cheng, J.-L Hu, J.-H Ren, H.-B Yu, S Zhong, V Wong, B Kwan Law, W.-X Chen, H.-M Xu, Z.-Z Zhang, X.-F Cai, Y Hu, W.- L Zhang, Q.-X Long, F Ren, H.-Z Zhou, A.-L Huang, J Chen, J Howell, A Pedrana, S Schroeder, N Scott, L Aufegger, R Atun, R Baptista-Leite, G Hirnschall, E 't Hoen, S Hutchinson, J Lazarus, L Olufunmilayo, R Peck, M Sharma, A Sohn, D Wilson, M Hellard, S.-Y Cai, D Yu, C Soroka, J Wang, J Boyer, D Chand, F Mohr, H Mcmillan, F Tukov, K Montgomery, A Kleyn, R Sun, S Tauscher-Wisniewski, P Kaufmann, G Kullak-Ublick, T Marjot, A Moon, J Cook, S Abd-Elsalam, C Aloman, M Armstrong, E Pose, E Brenner, T Cargill, M.-A Catana, R Dhanasekaran, A Eshraghian, I García-Juárez, U Gill, P Jones, J Kennedy, A Marshall, C Matthews, G Mells, C Mercer, P Perumalswami, E Avitabile, X Qi, F Su, N Ufere, Y Wong, M.-H Zheng, E Barnes, A Barritt, G Webb
Journal: Journal of Hepatology (2022)
STATE MEDICINE.
Journal: JAMA (1883)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Congenital anemia reveals distinct targeting mechanisms for master transcription factor GATA1
Authors: Leif S. Ludwig, Caleb A. Lareau, Erik L. Bao, Nan Liu, Taiju Utsugisawa, Alex M. Tseng, Samuel A. Myers, Jeffrey M. Verboon, Jacob C. Ulirsch, Wendy Luo, Christoph Muus, Claudia Fiorini, Meagan E. Olive, Christopher M. Vockley, Mathias Munschauer, Abigail Hunter, Hiromi Ogura, Toshiyuki Yamamoto, Hiroko Inada, Shinichiro Nakagawa, Shuichi Ohzono, Vidya Subramanian, Roberto Chiarle, Bertil Glader, Steven A. Carr, Martin J. Aryee, Anshul Kundaje, Stuart H. Orkin, Aviv Regev, Timothy L. McCavit, Hitoshi Kanno, Vijay G. Sankaran
Journal: Blood (2022)
Abnormal exocrine–endocrine cell cross-talk promotes β-cell dysfunction and loss in MODY8
Authors: Sevim Kahraman, Ercument Dirice, Giorgio Basile, Danielle Diegisser, Jahedul Alam, Bente B. Johansson, Manoj K. Gupta, Jiang Hu, Ling Huang, Chew-Li Soh, Danwei Huangfu, Senthil K. Muthuswamy, Helge Raeder, Anders Molven, Rohit N. Kulkarni
Journal: Nature Metabolism (2022)
Frequency and clinical significance of angiographic evidence of distal embolization during primary angioplasty for acute myocardial infarction Felix Zijlstra, Jan Paul Ottervanger, Menko Jan de Boer, Henk Eenhuizen, Dirk Dijkstra, Jan C.A. Hoorntje, Harry Suryapranata, Arnoud W.J. van 't Hof. Isala klinieken, hospital De Weezenlanden, Zwolle, The Netherlands
Journal: Journal of the American College of Cardiology (2001)
肠道中的微生物与黑色素瘤严重程度相关
Journal: British Journal of Dermatology (2022)

Abstract Linked Article: Vitali et al. Br J Dermatol 2022; 186:106–116. 黑色素瘤是一种由黑素细胞引起的皮肤癌 (黑素细胞能产生黑色素, 黑色素决定我们的皮肤颜色, 能够保护我们免受紫外线辐射损伤) 。这是一种严重的癌症, 在全球范围内导致最多的皮肤癌相关死亡。 与我们的身体一同存活的微生物 (真菌、酵母菌和细菌) 被称为“微生物群”。人类经过漫长进化, 已与我们的微生物群合作生存 (共生) 。 我们已知肠道微生物群能够与宿主免疫系统相互作用, 也知道黑色素瘤是一种强烈激活免疫系统的癌症, 因此, 我们进行了这项研究, 想要了解患者肠道中的微生物群是否与其疾病的严重程度有关。 我们使用一种称为二代测序的方法研究了肠道生态系统, 以识别所有微生物。本研究在意大利进行, 涉及黑色素瘤患者和健康对照参与者。 我们的研究结果表明, 黑色素瘤患者的肠道微生物群与健康对照参与者不同。此外, 在黑色素瘤患者中, 细菌和真菌群落的特征都与疾病的严重程度相关。我们得出结论, 早期黑色素瘤中的微生物群会随疾病严重程度而变化。我们的研究结果表明, 早期黑色素瘤研究的未来发展前景非常光明。 Linked Article: Vitali et al. Br J Dermatol 2022; 186:106–116.
Drug Treatment of Cluster Headache
Authors: Hans Christoph Diener, Arne May
Journal: Drugs (2021)

Cluster headache belongs to the group of trigeminal autonomic headaches. This review summarizes drug therapy of cluster attacks and prophylactic treatment. Neurostimulation methods are not addressed. The therapy for acute cluster attacks includes inhalation of 100% oxygen, subcutaneous administration of sumatriptan, and intranasal application of sumatriptan or zolmitriptan. Bridging therapy, which is used until oral prophylactic therapy is effective, is performed either with oral prednisolone or with a pharmacological block of the major occipital nerves. Best documented drugs for preventive treatment of cluster headache are verapamil and lithium, and possibly effective drugs are gabapentin, topiramate, divalproex sodium, and melatonin. The efficacy of monoclonal antibodies to the calcitonin gene-related peptide so far has been only demonstrated for episodic cluster headache. Several drug therapies are being investigated including ketamine, onabotulinumtoxinA, lysergic acid, and sodium oxybate.
Prospective study of the co-relation of ctDNA with pathologic complete remission (pCR) and other efficacy outcomes in rectal cancer patients undergoing neoadjuvant chemotherapy and radiation.
Authors: RuoBing Xue, Vivek R. Sharma, Russell Ware Farmer, Shesh Rai, Xiaoyong Wu, Michael Krainock, Bridgette Drummond, Perry Olshan, Paul R. Billings, Alexey Aleshin
Journal: Journal of Clinical Oncology (2022)

TPS235 Background: Circulating tumor DNA (ctDNA) has emerged as a biomarker for non-invasive longitudinal monitoring of tumor progression in cancer management. Through the recent advances in next generation sequencing (NGS) technologies and personalized assays, ctDNA has been heralded as a promising tool to detect residual disease, relapse, and monitor treatment response in hematologic malignancies and solid tumors. Our study aims to determine whether treatment related ctDNA dynamics can be used as a reliable indicator to predict pathologic complete response (pCR) in patients with rectal cancer receiving neoadjuvant treatment. Methods: This is a prospective observational cohort study. The primary aim is to estimate the sensitivity and specificity of ctDNA clearance in predicting pCR in patients undergoing neoadjuvant therapy. The secondary aim is to evaluate the feasibility of using ctDNA as a surveillance method to detect progression of rectal cancer during neoadjuvant therapy and relapse in the subsequent follow up period. ctDNA levels are collected from newly diagnosed rectal cancer patients at 7 discrete time points: at diagnosis or screening, during neoadjuvant therapy, after completion of neoadjuvant therapy and 1 month, 2 months, 4 months, 6 months after surgery. This will be followed by every 3 months ctDNA testing for surveillance for up to 2 years. We expect to enroll approximately 30 patients at our institution. The subjects will be sorted into two groups: responders and non-responders based on whether they achieve pCR. ctDNA level between two groups will subsequently be compared. The use of ctDNA to predict pCR in rectal cancer patients may allow for many of these patients to safely avoid surgery if undetectable ctDNA at the end of neoadjuvant therapy strongly correlates with pCR. We are actively enrolling patients into this prospective observational study and expect to report the data in the near future. The data we obtain will be combined with those from several institutions around the US doing similar studies with the same test. Data analysis will subsequently be conducted. [Table: see text]