tbuckley/cabot-search huggingface dataset:
Abstract 1920: Armed oncolytic myxoma virus demonstrates transgene production, function, and therapeutic activity xenograft models
Authors: Lina S. Franco, Mario Abrantes, Wazir Abdullahi, Ana L. de Matos, Benjamin S. Walker, Zachary Tacner, Cassandra Kien, Nicole Grigaitis, Grant McFadden, Steven J. Potts, Leslie L. Sharp
Journal: Cancer Research (2021)

Abstract Oncolytic viruses (OV) selectively replicate in and lyse tumor cells and provide stimulation to the immune system, representing a promising therapeutic option in development to treat cancers that do not respond well to treatment with immune checkpoint inhibitors. Myxoma virus (MYXV) is a member of the Pox family of double stranded DNA viruses. The natural host of MYXV is a subset of rabbits and hares, but MYXV is able to infect cancer cell lines of humans and other species. The genome of MYXV is relatively large and is amenable to engineering for expression of transgenic proteins, making it an excellent oncolytic virus for introduction of immunomodulatory proteins. Viral reproduction rates, transgene production, transgene function, and inhibition of cell growth of multi-armed myxoma virus was evaluated in vitro using cell lines representing multiple human cancer indications. Efficacy and mechanism of action of multi-armed myxoma virus was also demonstrated in vivo via xenograft models. Multi-armed myxoma represents a novel, engineerable, oncolytic virus with preclinical characteristics that warrant further investigation as an immunomodulatory cancer therapeutic. Citation Format: Lina S. Franco, Mario Abrantes, Wazir Abdullahi, Ana L. de Matos, Benjamin S. Walker, Zachary Tacner, Cassandra Kien, Nicole Grigaitis, Grant McFadden, Steven J. Potts, Leslie L. Sharp. Armed oncolytic myxoma virus demonstrates transgene production, function, and therapeutic activity xenograft models [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 1920.
Abstract 2377: microRNA expression profile in urinary exosomes is dependent on non-invasive lymphoma induction in mice
Authors: Brittany Wilson, Rebekah Betar, Alexander Martin, Zackaria Niazi, Michael Boyer, Lori Winter, Victor Babich, Francesca Di Sole, Elitsa Ananieva
Journal: Cancer Research (2021)

Abstract Lymphoma accounts for approximately 4% of cancers in the United States, with an estimated 20,910 number of deaths per year. The standard method of diagnosis and staging of lymphoma involves surgical biopsy of the tumor - a procedure that has many negative associated risks. Exosomes are extracellular vesicles secreted in biological fluids that can serve as “liquid biomarkers”. Identification of unique cancer-related biomarkers in urinary exosomes may provide a novel non-invasive and cost-effective tool for lymphoma diagnosis. Analyses of biomarkers obtained from urinary exosomes have been used to evaluate urological cancers, however, these methods have not yet been translated to non-urological pathologies, such as lymphomas. The objective for this study was to determine the profile of microRNAs (miRNAs) expressed in urinary exosomes of mice challenged with lymphoma and compare it to miRNAs identified in urinary exosomes of control mice. Male and female C57BL/6 mice, (tumor (+), n=12), were injected with either 2.5x105 mouse EL-4 lymphoma cells or phosphate-buffered saline (tumor (-), n=12). Tumor growth was monitored for 20 days. Urine was collected for 48 hours starting on day 17 and serum, tumor tissues, and organs were collected on day 20. Extraction of urinary exosomes was followed by total RNA isolation and RT-qPCR for which a set of PCR arrays consisting of 709 mouse-specific miRNA primers was used. Fold changes in miRNA expression were quantified using the ΔΔCt method. Mice developed tumors by day 13 with initial tumor appearance around day seven. There were no statistically significant differences between final tumor mass, body weight, or food and water intake in tumor (+) versus tumor (-) mice. RT-qPCR arrays of miRNAs extracted from urinary exosomes revealed 464 miRNAs that were differentially expressed between tumor (+) and tumor (-). 215 miRNAs were up-regulated, while 249 miRNAs were down-regulated in tumor (+) mice. These results will be compared to miRNAs from serum and tumor tissues to identify tumor-specific miRNAs that can be used for potential application in the clinical setting. Citation Format: Brittany Wilson, Rebekah Betar, Alexander Martin, Zackaria Niazi, Michael Boyer, Lori Winter, Victor Babich, Francesca Di Sole, Elitsa Ananieva. microRNA expression profile in urinary exosomes is dependent on non-invasive lymphoma induction in mice [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2377.
Neutralizing antibody response against variants of concern after vaccination of dialysis patients with BNT162b2
Authors: Claudius Speer, Louise Benning, Maximilian Töllner, Christian Nusshag, Florian Kälble, Paula Reichel, Matthias Schaier, Marie Bartenschlager, Paul Schnitzler, Martin Zeier, Caner Süsal, Christian Morath, Ralf Bartenschlager
Journal: Kidney International (2021)
Clinicopathological characteristics of upper tract urothelial cancer with loss of immunohistochemical expression of mismatch repair proteins
Authors: T. Hayashi, K. Ikeda, D. Taniyama, R.S. Hsi, S. Inoue, J. Teishima, S. Akabane, K. Sentani, W. Yasui, H. Yamamoto, K. Kuraoka, T. Hinoi
Journal: European Urology (2021)
Effect of dexamethasone on hepatic glucose and insulin metabolism after oral glucose in conscious dogs.
Authors: Z Chap, R H Jones, J Chou, C J Hartley, M L Entman, J B Field
Journal: Journal of Clinical Investigation (1986)

To examine whether hyperinsulinemia associated with glucocorticoid treatment results solely from hypersecretion of insulin or also involves altered fractional hepatic extraction, oral glucose (1 g/kg body wt) was administered to dogs with or without dexamethasone treatment (2 mg/d for 2 d). Dexamethasone significantly increased basal glucose and insulin concentrations in the portal vein, hepatic vein, and femoral artery, reduced basal fractional hepatic extraction of insulin from 43 +/- 4% to 22 +/- 4%, and, after oral glucose, increased retention by the liver of net glucose released into the portal system from 27 +/- 4% to 53 +/- 13%. Intraportal insulin infusion (1 and 2 mU/kg per min) after 7 d of dexamethasone treatment (2 mg/d) caused less suppression of endogenous glucose production, and less exogenous glucose was required to maintain an euglycemic clamp than in control animals. Dexamethasone treatment is associated with: decreased basal fractional hepatic insulin extraction contributing to hyperinsulinemia; and less suppression of endogenous glucose production and increase in peripheral uptake in response to insulin, but no reduction in net hepatic glucose uptake after oral glucose.
Ultrasound‐Powered Implants: A Critical Review of Piezoelectric Material Selection and Applications
Authors: Brendan L. Turner, Seedevi Senevirathne, Katie Kilgour, Darragh McArt, Manus Biggs, Stefano Menegatti, Michael A. Daniele
Journal: Advanced Healthcare Materials (2021)

Ultrasound-powered implants (UPIs) represent cutting edge power sources for implantable medical devices (IMDs), as their powering strategy allows for extended functional lifetime, decreased size, increased implant depth, and improved biocompatibility. IMDs are limited by their reliance on batteries. While batteries proved a stable power supply, batteries feature relatively large sizes, limited life spans, and toxic material compositions. Accordingly, energy harvesting and wireless power transfer (WPT) strategies are attracting increasing attention by researchers as alternative reliable power sources. Piezoelectric energy scavenging has shown promise for low power applications. However, energy scavenging devices need be located near sources of movement, and the power stream may suffer from occasional interruptions. WPT overcomes such challenges by more stable, on-demand power to IMDs. Among the various forms of WPT, ultrasound powering offers distinct advantages such as low tissue-mediated attenuation, a higher approved safe dose (720 mW cm-2 ), and improved efficiency at smaller device sizes. This study presents and discusses the state-of-the-art in UPIs by reviewing piezoelectric materials and harvesting devices including lead-based inorganic, lead-free inorganic, and organic polymers. A comparative discussion is also presented of the functional material properties, architecture, and performance metrics, together with an overview of the applications where UPIs are being deployed.
Surgical safety of Cytoreductive Nephrectomy (CN) following pretreatment with Immune Checkpoint Inhibition (ICI) combination therapy in primary metastatic clear-cell Renal Carcinoma (mccRCC)
Authors: N.M. Graafland, B. Szabados, F. Mumtaz, R. Barod, D. Nicol, C. Tanabalan, E. Boleti, T. Powles, J. Haanen, A. Bex
Journal: European Urology (2021)
Cutaneous vascular anomalies associated with a mosaic variant of AKT3: Genetic analysis continues to refine the diagnosis, nomenclature, and classification of vascular anomalies
Authors: Olivia M.T. Davies, Maria C. Garzon, Ilona J. Frieden, Catherine E. Cottrell, Karen W. Gripp, Russell P. Saneto, Tor Shwayder, Ghayda M. Mirzaa, Beth A. Drolet
Journal: Journal of the American Academy of Dermatology (2021)
Implantable Cardioverter-Defibrillator Eligibility After Initiation of Sacubitril/Valsartan in Chronic Heart Failure: Insights From PROVE-HF
Authors: G. Michael Felker, Javed Butler, Nasiren E. Ibrahim, Ileana L. Piña, Alan Maisel, Devavrat Bapat, Alexander Camacho, Jonathan H. Ward, Kristin M. Williamson, Scott D. Solomon, James L. Januzzi
Journal: Circulation (2021)
Abstract 994: Investigating inhibition of nuclear export in breast cancer cells
Authors: Robert F. Gruener, Geoffrey L. Greene, R. Stephanie Huang
Journal: Cancer Research (2021)

Abstract XPO1 (Exportin aka CRM1) is a mediator of nuclear export that is known to be dysregulated in cancers. Selective inhibitors of nuclear export (SINEs) have shown some promise in cancer treatment, but the mechanism is poorly understood in cancer specific subtypes. Here, we investigated XPO1 inhibition in breast cancer by treating a panel of breast cancer cell lines with a SINE (Selinexor) and saw a bimodal response, with most triple-negative cell lines showing sensitivity and some ER+ cell lines showing resistance to the therapy. Changes in the expression of BIRC5 (Survivin) have been previously implicated in XPO1 inhibitor response. Using RT-qPCR experiments we were able to show that after 24 hours of selinexor treatment, the expression of BIRC5 does change in two sensitive cell lines MDA-MB-231 and MCF7, but is not necessarily changed at earlier time points. To more holistically explore the mechanism of XPO1 inhibition induced cell death, we performed RNA-Seq and differential gene expression analysis on these two cell lines at 2, 8, and 24 hours post treatment with Selinexor (compared to vehicle). Our results indicate that several breast cancer and cell cycle associated genes show expression changes as early as 2 hours post-treatment and showed-consistent changes across the time-points. Overall, our analysis sheds light on the mechanism by which XPO1 inhibition induces cell death in breast cancer, which may help elucidate potential biomarkers as well as to nominate rationale combinations to explore with XPO1 inhibition. Citation Format: Robert F. Gruener, Geoffrey L. Greene, R. Stephanie Huang. Investigating inhibition of nuclear export in breast cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 994.
Where are the opportunities to address system barriers preventing equitable cardiovascular outcomes for indigenous populations?
Authors: Katharine F McBride, Anna Rolleston
Journal: The Lancet Global Health (2021)

The health and wellbeing of indigenous communities across the globe are affected by the substantial burden of cardiovascular disease.1–3 Burden disproportionally sits with younger indigenous people compared with non-indigenous populations, affecting the cultural, social, and economic strength of communities.
Prospective analysis of prostate cancer local staging with [18f] psma pet/mri combined with multiparametric mri – a comparison to histopathology in the radical prostatectomy specimen
Authors: Y.J.L. Bodar, G.J.C. Zwezerijnen, J.P. Van Der Voorn, B.H.E. Jansen, R.S. Smit, S.Q. Kol, D. Meijer, K. De Bie, N.H. Hendrikse, A.N. Vis, D.E. Oprea-Lager
Journal: European Urology (2021)
Abstract 14148: Combination Therapy of an Angiotensin Receptor Blocker and a Calcium Channel Blocker Ameliorated Vascular Insulin Resistance in Metabolic Syndrome Rats Synergistically via a Reduction in Adipocyte Size and an Anti-Inflammatory Effect
Authors: Daisuke Sueta, Taishi Nakamura, Keiichiro Kataoka, Nobutaka Koibuchi, Kensuke Toyama, Yi-Fei Dong, Hisao Ogawa, Shokei Kim-Mitsuyama
Journal: Circulation (2011)

[Aim] The significance of combination therapy of angiotensin receptor blocker (ARB)- and calcium channel blocker (CCB) for metabolic syndrome remains to be elucidated. We examined the efficacy of A...
Abstract 9877: Novel Clinical Risk Score for the Prediction of Cardiac Events in Patients with Vasospastic Angina -A Report from the Nationwide Multi-Center Registry by the Japanese Coronary Spasm Association
Authors: Yusuke Takagi, Satoshi Yasuda, Ryusuke Tsunoda, Yasuhiro Ogata, Atsushi Seki, Tetsuya Sumiyoshi, Motoyuki Matsui, Toshikazu Goto, Yasuhiko Tanabe, Shozo Sueda, Toshiaki Sato, Satoshi Ogawa, Norifumi Kubo, Shin-ichi Momomura, Hisao Ogawa, Hiroaki Shimokawa
Journal: Circulation (2011)

Background: Previous studies demonstrated various predictive factors of cardiac events (e.g., out-of-hospital cardiac arrest) in patients with vasospastic angina (VSA). However, the useful score to predict cardiovascular events in VSA patients needs to be developed. We thus aimed to develop a novel clinical risk prediction score for VSA patients. Methods: The VSA database of the nationwide multi-center registry study by the Japanese Coronary Spasm Association (n=1,429; median 66 years; median follow-up of 32 months) was utilized for score derivation. Multivariable Cox proportional hazard model was used to select the correlated factors of major adverse cardiac events (MACE) including cardiac death, non-fatal myocardial infarction, unstable angina and heart failure. They were subsequently weighted according to the adjusted hazard ratio (HR) and integrated into the scoring system. Results: Six variables selected following Cox model were weighted; smoking (2 points), angina at rest alone (2 points), life-threatening arrhythmia during angina (2 points), multivessel spasm (2 points), organic coronary stenosis &gt;50% (2 points) and ST elevation during angina (1 point). According to the total score, 3 strata of risk were defined; low (score 0-3, n=713), intermediate (score 4-6, n=666) and high (score 7-9, n=50). The incidence of MACE in the low-, intermediate- and high-risk patients were 2.8%, 8.3% and 20.0%, respectively (P&lt;0.001). Kaplan-Meier curve for MACE among the 3 risk groups showed prognostic utility of the scoring system throughout the follow-up period (low vs. intermediate, HR 3.04, 95% CI 1.82-5.07, P&lt;0.001; low vs. high, HR 8.13, 95% CI 3.80-17.37, P&lt;0.001) ( Figure ). The C-statistic for the score predicting MACE was 0.67. Conclusions: We developed a novel scoring system that provides the stratification of cumulative risk of cardiac events in VSA patients, which is clinically relevant in the triage and management of the disorder.
Abstract 2796: Pan-cancer characterization of tumor immune microenvironment
Authors: Tianqing Chu, Zhang Bei
Journal: Cancer Research (2021)

Abstract Background: Anti-PD1/PDL1 therapy has been established as standard care in multiple tumor types, however, many patients will not benefit from this treatment as lack of infiltrating immune cells. Our aim was to explore the pan-cancer immune microenvironment and whether they impacted by ICI biomarkers. Method: 7-plex mIHC was performed to assessed immune cell infiltrations were assessed. Positive PD-L1 expression was defined as ≥1% of tumor cells with membranous staining. Statistical analyses were performed using R version 3.4.4. Result: Among 520 samples, CD8+T cell infiltration in tumor was assessed in Esophagus, Liver, Biliary Tract, Lung, Colorectum, Stomach tumors, ordered by descending median density at the center of the tumor. The median density at the periphery was almost double than the median density at the center. In addition, we have observed high TAM.M1 density in Bladder urinary Tract, Biliary Tract, Liver, with a higher or equivalent level of infiltration in stroma than in tumor. While in contrast, the TAM.M2 cell density was higher in stroma than in tumor. Regarding to PDL1 status in colorectum, there was little difference in the degree of CD8+ T cell infiltration between stroma and tumor. In the term of MSI status, CD8+T cell infiltration was significantly higher in MSIH colorectum tumor. We compared the proportion of above immune cells. In stroma, except lung tumor, CD8+T cells made up the largest share of immune cells, following by NK cells. In tumor, except for lung cancer, NK cells accounted for the highest proportion. Cancer with the highest proportion of NK cells is bladder/urinary tract cancer. Although the proportion of NK cells in lung cancer is also high, macrophages proportion is the highest, especially TAM.M2. Conclusions: Together, these data outlined the immune infiltration inter-, extra-tumor, and associations with TMB, MSI status. Tumor immune microenvironment might serve as promising predictive factors for immunotherapy. Citation Format: Tianqing Chu, Zhang Bei. Pan-cancer characterization of tumor immune microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2796.
Holmium laser enucleation of prostate versus minimally invasive simple prostatectomy for large volume (≥ 120 ml) prostate glands: A prospective multicenter randomized study
Authors: A.L. Pastore, A. Fuschi, Y. Al Salhi, L. Capone, F. Annino, S. Khorrami, A. Asimakopoulos, M. Falsaperla, G. Bozzini, A. Carbone
Journal: European Urology (2021)
An in vitro analysis of holmium: YAG and thulium fiber laser ablation rates
Authors: M. Basulto-Martínez, S. Proietti, M.P. Pavia, L. Rico, Y. Yeow, J.P. Flores-Tapia, A. Esqueda-Mendoza, F. Gaboardi, G. Giusti
Journal: European Urology (2021)
Abstract 2745: Murine high grade serous ovarian cancer (HGSOC) models replicate the human tumor microenvironment and suggest anti-tumor activities of macrophages after chemotherapy
Authors: Chiara Berlato, Owen Heath, Eleni Maniati, Ganga Gopinathan, Panoraia Kotantaki, Samar Elorbany, Jacqueline McDermott, Colin Pegrum, Ludmila Szabova, Ruth Perets, Ronny Drapkin, Peter Bailey, Alessandro Annibaldi, Desmond P. Barton, Frances R. Balkwill
Journal: Cancer Research (2021)

Abstract The aim of this study is to characterize and validate similarities between the human tumor microenvironment (TME) and the TME of new HGSOC murine models and to use the models to analyze the role of tumor associated macrophages (TAMs) after chemotherapy. HGSOC has seen in the past decades only a modest reduction of mortality. This is in part due to the lack of suitable models, as the origin and mutation patterns of HGSOC were not previously understood. Despite recent advances with organoid models, murine models that represent the TME and in particular the complex interactions with the immune system are needed. We characterized the TME of six orthotopic, transplantable syngeneic murine HGSOC lines established from tumors in genetic models and compared these to patient biopsies. This analysis identified significant correlations between the transcriptome, host cell infiltrates, immune response, matrisome, vasculature and tissue modulus of mouse and human TMEs, with several stromal and malignant cell targets in common. However, each model also showed distinct differences and potential vulnerabilities that will enable us to study potential therapies. These models allowed us to extend our understanding of the role of TAMs following chemotherapy. Chemotherapy reduced the number and changed the phenotype of TAMs both in patient biopsies and in mouse models. Following chemotherapy, inflammasome activation in TAMs was also observed. Depletion of TAMs with CSFR1 inhibitors after carboplatin treatment reduced survival in a mouse model, suggesting that chemotherapy can activate sub-populations of macrophages to an anti-tumor phenotype that can sustain adaptive immunity. In conclusion, we have characterized murine transplantable models that share similarities with human HGSOC and have helped us elucidate the anti-tumor role of TAMs once activated by chemotherapy. Citation Format: Chiara Berlato, Owen Heath, Eleni Maniati, Ganga Gopinathan, Panoraia Kotantaki, Samar Elorbany, Jacqueline McDermott, Colin Pegrum, Ludmila Szabova, Ruth Perets, Ronny Drapkin, Peter Bailey, Alessandro Annibaldi, Desmond P. Barton, Frances R. Balkwill. Murine high grade serous ovarian cancer (HGSOC) models replicate the human tumor microenvironment and suggest anti-tumor activities of macrophages after chemotherapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2745.
IFN-γ Induces Distinct Single Cell Transcriptomes in Clinical-Grade Mesenchymal Stromal Cells
Authors: Reema Panjwani, Stefan Cordes, Zerick Terrell Dunbar, Jinguo Chen, Prachi Jain, Fariba Chinian, Sara Hauffe, Eleftheria Koklanaris, Jeanine Superata, Rongye Shi, Huizhi Zhou, David Stroncek, Nan Zhang, Sarah Davies, Minoo Battiwalla, Austin John Barrett, Sawa Ito
Journal: Blood (2017)
Using a curated semi-automated algorithm for target identification in muscle-invasive bladder cancer
Authors: F.A. Krentel, F. Singer, L. Rosano Gonzalez, E.A. Gibb, Y. Liu, E. Davicioni, D.J. Stekhoven, M. Kruithof-De Julio, R. Seiler-Blarer
Journal: European Urology (2021)
Current Advances in Photoactive Agents for Cancer Imaging and Therapy
Authors: Deanna Broadwater, Hyllana C.D. Medeiros, Richard R. Lunt, Sophia Y. Lunt
Journal: Annual Review of Biomedical Engineering (2021)

Photoactive agents are promising complements for both early diagnosis and targeted treatment of cancer. The dual combination of diagnostics and therapeutics is known as theranostics. Photoactive theranostic agents are activated by a specific wavelength of light and emit another wavelength, which can be detected for imaging tumors, used to generate reactive oxygen species for ablating tumors, or both. Photodynamic therapy (PDT) combines photosensitizer (PS) accumulation and site-directed light irradiation for simultaneous imaging diagnostics and spatially targeted therapy. Although utilized since the early 1900s, advances in the fields of cancer biology, materials science, and nanomedicine have expanded photoactive agents to modern medical treatments. In this review we summarize the origins of PDT and the subsequent generations of PSs and analyze seminal research contributions that have provided insight into rational PS design, such as photophysics, modes of cell death, tumor-targeting mechanisms, and light dosing regimens. We highlight optimizable parameters that, with further exploration, can expand clinical applications of photoactive agents to revolutionize cancer diagnostics and treatment.
マンギフェリンによるPINK1-PRKNマイトファジー抑制はマウスC3H10T1/2間葉系幹細胞におけるPKA-p38 MAPKシグナリングを介して褐色脂肪表現型を促進する【JST・京大機械翻訳】
Authors: Rahman Md. Shamim, Kim Yong-Sik
Journal: Metabolism (2020)