tbuckley/cabot-search huggingface dataset:
Abstract P3-06-02: Exosomes produced by adipocytes induce EMT, immune exhaustion and tumor metastasis, in both <i>in vivo</i> and <i>in vitro</i> models of TNBC
Authors: Yuhan Qiu, Conor Ross, Naser Jafari, Manohar Kolla, Pablo Llevenes, Christina Ennis, Carla S Mazzeo, Kiana Mahdaviani, Naomi Y Ko, Gerald V Denis
Journal: Cancer Research (2022)

Abstract Type 2 Diabetes (T2D) is a chronic inflammatory disease characterized by inflamed adipose tissue. Patients with triple negative breast cancer (TNBC) and comorbid T2D have higher risk of metastasis and shorter survival. However, mechanisms that couple T2D to TNBC outcomes are unknown. Here we hypothesize that exosomes, small vesicles secreted by tumor microenvironment breast adipocytes, drive epithelial-to-mesenchymal transition (EMT) in TNBC, immune exhaustion and metastasis via Ampk-Akt signaling.Methods: Exosomes were purified from conditioned media of 3T3-L1 mature adipocytes that were insulin-sensitive (IS) or insulin-resistant (IR), then characterized and quantified by NanoSight and surface markers, CD63. Murine 4T1 cells, a TNBC model, were treated with exosomes in vitro (3 days). To establish 3D organoids, 4T1 spheroids were treated with exosomes and embedded in Matrigel. For in vivo models, mammary fat pads of BALB/c mice were injected with 4T1 cells treated with IS vs IR exosomes. 4T1 tumors were harvested and mRNA extracted for qPCR, RNAseq, and Ingenuity Pathway Analysis. Metastatic sites in lung and liver were visualized by H&amp;E staining and clonogenic assay. Immune exhaustion markers of tumor infiltrated lymphocytes (TILs) were measured by flow cytometry. Results: In 4T1 cells treated with IR exosomes, EMT was upregulated and PD-L1 expression increased. Tumor-bearing mice exhibited metastasis in exosome-treated groups, visualized by microscopy and clonogenic assay. Immune exhaustion markers showed modified expression in TILs from exosome-treated groups. RNA-seq analysis revealed differences among exosome-treated groups that suggest dysregulated Ampk-Akt pathways.Conclusion: Exosomes from IR adipocytes modify the tumor microenvironment, increase EMT and immune exhaustion markers on tumor cells and TILs, and promote metastasis to distant organs through Ampk-Akt. Metabolic diseases such as T2D reshape the TNBC tumor microenvironment, promoting metastasis and decreasing survival. Clearly, TNBC patients with T2D should be more closely monitored for metastasis than metabolically normal patients, with metabolic medications considered. Citation Format: Yuhan Qiu, Conor Ross, Naser Jafari, Manohar Kolla, Pablo Llevenes, Christina Ennis, Carla S Mazzeo, Kiana Mahdaviani, Naomi Y Ko, Gerald V Denis. Exosomes produced by adipocytes induce EMT, immune exhaustion and tumor metastasis, in both in vivo and in vitro models of TNBC [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr P3-06-02.
Humoral response to SARS-CoV-2 infection among liver transplant recipients
Authors: Chiara Becchetti, Annelotte G C Broekhoven, Géraldine Dahlqvist, Montserrat Fraga, Marco Fabrizio Zambelli, Olga Ciccarelli, Anne-Catherine Saouli, Arianna Trizzino, Vanessa Banz, Jean-François Dufour, Anna H E Roukens, Shessy P Torres Morales, Sebenzile K Myeni, Marjolein Kikkert, Mariet C W Feltkamp, Minneke J Coenraad
Journal: Gut (2022)

Immunosuppressive agents are known to interfere with T and/or B lymphocytes, which are required to mount an adequate serologic response. Therefore, we aim to investigate the antibody response to SARS-CoV-2 in liver transplant (LT) recipients after COVID-19.Prospective multicentre case-control study, analysing antibodies against the nucleocapsid protein, spike (S) protein of SARS-CoV-2 and their neutralising activity in LT recipients with confirmed SARS-CoV-2 infection (COVID-19-LT) compared with immunocompetent patients (COVID-19-immunocompetent) and LT recipients without COVID-19 symptoms (non-COVID-19-LT).Overall, 35 LT recipients were included in the COVID-19-LT cohort. 35 and 70 subjects fulfilling the matching criteria were assigned to the COVID-19-immunocompetent and non-COVID-19-LT cohorts, respectively. We showed that LT recipients, despite immunosuppression and less symptoms, mounted a detectable antinucleocapsid antibody titre in 80% of the cases, although significantly lower compared with the COVID-19-immunocompetent cohort (3.73 vs 7.36 index level, p<0.001). When analysing anti-S antibody response, no difference in positivity rate was found between the COVID-19-LT and COVID-19-immunocompetent cohorts (97.1% vs 100%, p=0.314). Functional antibody testing showed neutralising activity in 82.9% of LT recipients (vs 100% in COVID-19-immunocompetent cohort, p=0.024).Our findings suggest that the humoral response of LT recipients is only slightly lower than expected, compared with COVID-19 immunocompetent controls. Testing for anti-S antibodies alone can lead to an overestimation of the neutralising ability in LT recipients. Altogether, routine antibody testing against separate SARS-CoV-2 antigens and functional testing show that the far majority of LT patients are capable of mounting an adequate antibody response with neutralising ability.
Two years of COVID-19: many lessons, but will we learn?
Authors: David L. Heymann, Helena Legido-Quigley
Journal: Eurosurveillance (2022)

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RATE OF 30-DAY READMISSION AND ECONOMIC BURDEN IN PATIENTS WITH ATRIAL FIBRILLATION AND CO-EXISTING PROTEIN ENERGY MALNUTRITION
Authors: Michael A. Fatuyi, Leanne Pereira, Vahid Namdarizandi, Awfa Zain Elabidin, Vivek Sharma, Leonid Khokhlov, Mubarak H. Yusuf, Oghenesuvwe Eboh, Asimul H. Ansari, Kamal Shemisa
Journal: Journal of the American College of Cardiology (2022)
What Is Rheumatoid Arthritis?
Authors: Melanie H. Smith, Jessica R. Berman
Journal: JAMA (2022)

Signe Møller-Bisgaard, MD, PhD; Kim Hørslev-Petersen, MD, DMSc; Bo Ejbjerg, MD, PhD; Merete Lund Hetland, MD, PhD, DMSc; Lykke Midtbøll Ørnbjerg, MD, PhD; Daniel Glinatsi, MD, PhD; Jakob Møller, MSc; Mikael Boesen, MD, PhD; Robin Christensen, MSc, PhD; Kristian Stengaard-Pedersen, MD, DMSc; Ole Rintek Madsen, MD, PhD, DMSc; Bente Jensen, MD, PhD; Jan Alexander Villadsen, MD; Ellen-Margrethe Hauge, MD, PhD; Philip Bennett, MD; Oliver Hendricks, MD, PhD; Karsten Asmussen, MD, PhD; Marcin Kowalski, MD, PhD; Hanne Lindegaard, MD, PhD; Sabrina Mai Nielsen, MSc; Henning Bliddal, MD, DMSc; Niels Steen Krogh, MSc; Torkell Ellingsen, MD, PhD; Agnete H. Nielsen, MD; Lone Balding, MD; Anne Grethe Jurik, MD, DMSc; Henrik S. Thomsen, MD, DMSc; Mikkel Østergaard, MD, PhD, DMSc
Expert consensus on dental caries management
Authors: Lei Cheng, Lu Zhang, Lin Yue, Junqi Ling, Mingwen Fan, Deqin Yang, Zhengwei Huang, Yumei Niu, Jianguo Liu, Jin Zhao, Yanhong Li, Bin Guo, Zhi Chen, Xuedong Zhou
Journal: International Journal of Oral Science (2022)

Dental Caries is a kind of chronic oral disease that greatly threaten human being's health. Though dentists and researchers struggled for decades to combat this oral disease, the incidence and prevalence of dental caries remain quite high. Therefore, improving the disease management is a key issue for the whole population and life cycle management of dental caries. So clinical difficulty assessment system of caries prevention and management is established based on dental caries diagnosis and classification. Dentists should perform oral examination and establish dental records at each visit. When treatment plan is made on the base of caries risk assessment and carious lesion activity, we need to work out patient‑centered and personalized treatment planning to regain oral microecological balance, to control caries progression and to restore the structure and function of the carious teeth. And the follow-up visits are made based on personalized caries management. This expert consensus mainly discusses caries risk assessment, caries treatment difficulty assessment and dental caries treatment plan, which are the most important parts of caries management in the whole life cycle.
Coupling of angiogenesis and odontogenesis orchestrates tooth mineralization in mice
Authors: Tomoko Matsubara, Takahito Iga, Yuki Sugiura, Dai Kusumoto, Tsukasa Sanosaka, Ikue Tai-Nagara, Norihiko Takeda, Guo-Hua Fong, Kosei Ito, Masatsugu Ema, Hideyuki Okano, Jun Kohyama, Makoto Suematsu, Yoshiaki Kubota
Journal: The Journal of Experimental Medicine (2022)

The skeletal system consists of bones and teeth, both of which are hardened via mineralization to support daily physical activity and mastication. The precise mechanism for this process, especially how blood vessels contribute to tissue mineralization, remains incompletely understood. Here, we established an imaging technique to visualize the 3D structure of the tooth vasculature at a single-cell level. Using this technique combined with single-cell RNA sequencing, we identified a unique endothelial subtype specialized to dentinogenesis, a process of tooth mineralization, termed periodontal tip-like endothelial cells. These capillaries exhibit high angiogenic activity and plasticity under the control of odontoblasts; in turn, the capillaries trigger odontoblast maturation. Metabolomic analysis demonstrated that the capillaries perform the phosphate delivery required for dentinogenesis. Taken together, our data identified the fundamental cell-to-cell communications that orchestrate tooth formation, angiogenic-odontogenic coupling, a distinct mechanism compared to the angiogenic-osteogenic coupling in bones. This mechanism contributes to our understanding concerning the functional diversity of organotypic vasculature.
Serology Test Results and Other Important Characteristics of Patients With Persistent COVID-19 Symptoms
Authors: Michiel Tack
Journal: JAMA Internal Medicine (2022)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
A NOVEL PREDICTIVE MODEL OF CORONARY ARTERIAL INLET, OUTLET AND SIDE-BRANCH BLOOD FLOW
Authors: Daniel Taylor, Paul David Morris, Julian Gunn, Rebecca Gosling, Ian Halliday, David R. Hose, Vignesh Rammohan, Marcel van‘t Veer, Pim A. Tonino, Daniëlle Keulards, Jeroen Feher, Michel Rochette
Journal: Journal of the American College of Cardiology (2022)
NON-INVASIVE AND AUTOMATED CARDIAC OUTPUT MEASUREMENT FROM CARDIAC ACOUSTIC SIGNALS USING THE CARDIAC PERFORMANCE SYSTEM: A COMPARISON WITH CATHETERIZATION
Authors: Harsh Golwala, Firas E. Zahr, Kelsey Cameron, Yi Zheng, Christopher I. Baek, Kanav Saraf, Michael H. Wasko, Xu Zhang, Per H. Borgstrom, William Kaiser
Journal: Journal of the American College of Cardiology (2022)
AHA LIFE’S SIMPLE 7 METRICS ARE NOT SIGNIFICANTLY ASSOCIATED WITH SUBCLINICAL CORONARY ATHEROSCLEROSIS INCIDENCE OR PROGRESSION AMONG SOUTH ASIANS OR OTHER RACIAL/ETHNIC GROUPS: FINDINGS FROM THE MASALA AND MESA STUDIES
Authors: Vardhmaan Jain, mahmoud AlRifai, Alka Kanaya, Nilay S. Shah, Sameera Talegawkar, Kevin Shah, Salim S. Virani, Erin D. Michos, Roger S. Blumenthal, Jaideep Patel
Journal: Journal of the American College of Cardiology (2022)
Abstract PD3-09: Cd73 may influence zoledronate-induced b- and t-cell accumulation into triple-negative breast tumors
Authors: Nataliia Petruk, Arafat Siddiqui, Jorma Määttä, Jouko Sandholm, Katri Selander
Journal: Cancer Research (2022)

Abstract Background: CD73 is an ecto-5′-nucleotidase, which converts AMP to adenosine. In breast cancer, CD73 expression is associated with prognosis, treatment responses to anthracyclines and tumor immunity. Bisphosphonates (BP) inhibit osteoclast-mediated bone resorption. When given in the adjuvant setting, BPs increase survival among post-menopausal breast cancer patients. Since BPs also affect cells of the immune system, we hypothesized that CD73 expression may affect BP responses in triple negative breast cancer cells. Methods: Cell viability and IC50 BPs doses were determined by WST-8 kit. Apoptotic rate and cell cycle arrest were showed by FACS. The number of caspase3/7- positive cells was accessed by a red fluorescence emission via IncuCyteS3. The apoptosis-associated genes mRNA expression was determined by qPCR. To compare BP effect on TNBC tumoral responses in vivo, we used immune-competent mouse models of breast cancer. Tumors were stained by immunofluorescence and immunohistochemically.Results: Suppression of CD73 expression, but not of enzymatic activity significantly sensitized TNBC cells to nitrogen-containing BPs (n-BPs), especially to zoledronate in vitro. The stronger growth inhibitory effects of n-BPs in CD73-suppressed cells were associated with G1 cell cycle arrest and increased apoptosis, as compared with representative control cells in vitro. Zoledronate similarly inhibited both control and CD73-suppressed TNBC tumor growthin immune-competent mice. CD73 suppression in tumors led to the lower lung metastatic burden and metastases size. Zoledronate effect on increased apoptosis was similar in both groups, but significant in CD73-suppressed tumors. Impaired angiogenesis was shown by decreased CD34-positive cell number in CD73-suppressed tumors. Zoledronate significantly increased infiltration of CD45R/B220+ B cells and cytotoxic CD8+ T cells into CD73-suppressed tumors.Conclusions: Suppressed CD73 sensitizes TNBC cells to n-BP treatment, resulting inapoptosis activation and delayed proliferation in vitro. Zoledronate induces a more profound accumulation of CD45R/B220+ B cells and CD8+ T cells into TNBC tumors with the lack of CD73. Since tumor B-cell infiltration has been associated with favourable patient prognosis in breast cancer, the significance of this B-cell effect of zoledronate warrants further investigation. Citation Format: Nataliia Petruk, Arafat Siddiqui, Jorma Määttä, Jouko Sandholm, Katri Selander. Cd73 may influence zoledronate-induced b- and t-cell accumulation into triple-negative breast tumors [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr PD3-09.
Medical Groups Defend Patient-Physician Relationship and Access to Adolescent Gender-Affirming Care
Authors: Bridget M. Kuehn
Journal: JAMA (2022)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
HSR22-167: Burden of Myelosuppression Among Patients With Extensive-Stage Small Cell Lung Cancer Treated With Chemotherapy in a Community Oncology Setting
Authors: Jeffrey Scott, Dean Slack, Lindsay Gingras, Marc Chioda, Huan Huang
Journal: Journal of the National Comprehensive Cancer Network (2022)

"HSR22-167: Burden of Myelosuppression Among Patients With Extensive-Stage Small Cell Lung Cancer Treated With Chemotherapy in a Community Oncology Setting" published on 31 Mar 2022 by National Comprehensive Cancer Network.
PURE ADRENALINE: A CASE REPORT OF EXCELLENT OUTCOME OF HIGH RISK REVASCULARIZATION IN A NONAGENARIAN FEMALE.
Authors: Alejandro Sanchez, Alejandra Diaz-Sierra, Muftawu-Deen Iddrisu, Antonio Lewis, Eduardo Javier Perez, Andres Sanchez Nadales, Jose Sleiman
Journal: Journal of the American College of Cardiology (2022)
A multicenter evaluation of computable phenotyping approaches for SARS-CoV-2 infection and COVID-19 hospitalizations
Authors: Rohan Khera, Bobak J. Mortazavi, Veer Sangha, Frederick Warner, H. Patrick Young, Joseph S. Ross, Nilay D. Shah, Elitza S. Theel, William G. Jenkinson, Camille Knepper, Karen Wang, David Peaper, Richard A. Martinello, Cynthia A. Brandt, Zhenqiu Lin, Albert I. Ko, Harlan M. Krumholz, Benjamin D. Pollock, Wade L. Schulz
Journal: npj Digital Medicine (2022)

Abstract Diagnosis codes are used to study SARS-CoV2 infections and COVID-19 hospitalizations in administrative and electronic health record (EHR) data. Using EHR data (April 2020–March 2021) at the Yale-New Haven Health System and the three hospital systems of the Mayo Clinic, computable phenotype definitions based on ICD-10 diagnosis of COVID-19 (U07.1) were evaluated against positive SARS-CoV-2 PCR or antigen tests. We included 69,423 patients at Yale and 75,748 at Mayo Clinic with either a diagnosis code or a positive SARS-CoV-2 test. The precision and recall of a COVID-19 diagnosis for a positive test were 68.8% and 83.3%, respectively, at Yale, with higher precision (95%) and lower recall (63.5%) at Mayo Clinic, varying between 59.2% in Rochester to 97.3% in Arizona. For hospitalizations with a principal COVID-19 diagnosis, 94.8% at Yale and 80.5% at Mayo Clinic had an associated positive laboratory test, with secondary diagnosis of COVID-19 identifying additional patients. These patients had a twofold higher inhospital mortality than based on principal diagnosis. Standardization of coding practices is needed before the use of diagnosis codes in clinical research and epidemiological surveillance of COVID-19.
SYNERGY- EVEROLIMUS ELUTING STENT WITH A BIOABSORBABLE POLYMER IN ST ELEVATION MYOCARDIAL INFARCTION- CLEAR SYNERGY OASIS-9 TRIAL
Authors: Sanjit S. Jolly, Rajibul Mian, Sasko Kedev, Shahar Lavi, Raul Moreno, Gilles Montalescot, Timothy D. Henry, Valon Asani, Robert F. Storey, Johanne Silvain, James C.S. Spratt, Goran Stankovic, Biljana Zafirovska, Madhu K. Natarajan, Manel Sabate, Satya Shreenivas, Omar Abdul-Jawad Altisent, Núria Ribas, Shamir R. Mehta
Journal: Journal of the American College of Cardiology (2022)
Association of Cardiometabolic Disease With Cancer in the Community
Authors: Elizabeth E. Liu, Navin Suthahar, Samantha M. Paniagua, Dongyu Wang, Emily S. Lau, Shawn X. Li, Manol Jovani, Katherine S. Takvorian, Bernard E. Kreger, Emelia J. Benjamin, Wouter C. Meijers, Stephan J.L. Bakker, Lyanne M. Kieneker, Eke G. Gruppen, Bert van der Vegt, Geertruida H. de Bock, Ron T. Gansevoort, Shehnaz K. Hussain, Udo Hoffmann, Greta Lee Splansky, Ramachandran S. Vasan, Martin G. Larson, Daniel Levy, Susan Cheng, Rudolf A. de Boer, Jennifer E. Ho
Journal: JACC CardioOncology (2022)

Obesity and cardiometabolic dysfunction have been associated with cancer risk and severity. Underlying mechanisms remain unclear.The aim of this study was to examine associations of obesity and related cardiometabolic traits with incident cancer.FHS (Framingham Heart Study) and PREVEND (Prevention of Renal and Vascular End-Stage Disease) study participants without prevalent cancer were studied, examining associations of obesity, body mass index (BMI), waist circumference, visceral adipose tissue (VAT) and subcutaneous adipose tissue depots, and C-reactive protein (CRP) with future cancer in Cox models.Among 20,667 participants (mean age 50 years, 53% women), 2,619 cancer events were observed over a median follow-up duration of 15 years. Obesity was associated with increased risk for future gastrointestinal (HR: 1.30; 95% CI: 1.05-1.60), gynecologic (HR: 1.62; 95% CI: 1.08-2.45), and breast (HR: 1.32; 95% CI: 1.05-1.66) cancer and lower risk for lung cancer (HR: 0.62; 95% CI: 0.44-0.87). Similarly, waist circumference was associated with increased risk for overall, gastrointestinal, and gynecologic but not lung cancer. VAT but not subcutaneous adipose tissue was associated with risk for overall cancer (HR: 1.22; 95% CI: 1.05-1.43), lung cancer (HR: 1.92; 95% CI: 1.01-3.66), and melanoma (HR: 1.56; 95% CI: 1.02-2.38) independent of BMI. Last, higher CRP levels were associated with higher risk for overall, colorectal, and lung cancer (P < 0.05 for all).Obesity and abdominal adiposity are associated with future risk for specific cancers (eg, gastrointestinal, gynecologic). Although obesity was associated with lower risk for lung cancer, greater VAT and CRP were associated with higher lung cancer risk after adjusting for BMI.
Meet the First Authors
Authors: Audrys Pauža
Journal: Circulation Research (2022)

After earning his MS in Neuroscience from Vilnius University in 2015, he went on to receive a four-year BHF-PhD scholarship to investigate the mechanisms of chemosensory sensitization in hypertension.His main research interests are the autonomic control of circulation and low metabolic states (hibernation/torpor), in particular, the integration of high-throughput omics data with the functional systems-level physiology to find novel treatments for cardiovascular and metabolic disease.After his PhD, he plans to continue his studies as a postdoctoral fellow at the University of Auckland in New Zealand.He can be found on Twitter @audrysgp.