Decline and loss of anti–SARS-CoV-2 antibodies in kidney transplant recipients in the 6 months following SARS-CoV-2 infection
Authors: Nathalie Chavarot, Marianne Leruez-Ville, Anne Scemla, Carole Burger, Lucile Amrouche, Claire Rouzaud, Xavier Lebreton, Frank Martinez, Rebecca Sberro-Soussan, Christophe Legendre, Julien Zuber, Dany Anglicheau
Journal: Kidney International (2021)

The dynamics of immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in kidney transplant recipients (KTRs) remains largely unknown. KTRs have been reported to develop serological responses to SARS-CoV-2.1Prendecki M. Clarke C. Gleeson S. et al.Detection of SARS-CoV-2 antibodies in kidney transplant recipients.J Am Soc Nephrol. 2020; 31: 2753-2756Crossref PubMed Scopus (28) Google Scholar,2Choi M. Bachmann F. Naik M.G. et al.Low seroprevalence of SARS-CoV-2 antibodies during systematic antibody screening and serum responses in patients after COVID-19 in a German transplant center.J Clin Med. 2020; 9: 3401Crossref Scopus (11) Google Scholar However, information about the duration and significance of antibody response in this immunocompromised population is still critically lacking. We herein report anti–SARS-CoV-2 IgG trajectory in a cohort of KTRs followed at Necker Hospital (Paris, France) between 2 and 6 months after symptomatic coronavirus disease 2019 (COVID-19) infection. Forty-two patients (22 men [52.4%]; median age of 57.7 years; interquartile range [IQR]: 47.2–67.0), who developed COVID-19 infection between March 14 and May 2, 2020, were included. COVID-19 was defined by typical clinical symptoms associated to a positive SARS-CoV-2 polymerase chain reaction test on nasopharyngeal swab. Sera were tested for the presence of anti-nucleocapsid protein IgG by a chemiluminescent microparticle immunoassay (SARS-CoV-2 IgG assay, Abbott, Abbott Park, IL) at 2 and 6 months after COVID-19 onset. According to the manufacturer's instructions, an IgG index >1.4 indicates a positive serology while an IgG index between 0.4 and 1.4 is considered to be an equivocal result and IgG index <0.4 to be a negative result. Sera were available for all patients at month 2 and for 33 of 42 patients at month 6. COVID-19 occurred at a median time of 6.3 years (IQR: 3.1–12.7) after transplantation. In our cohort, 32 patients (76.2%) required hospitalization, including 7 (21.9%) in an intensive care unit (ICU), none of whom died. At first serological testing (month 2), all patients had recovered and SARS-CoV-2 polymerase chain reaction was negative in 40 patients (95.2%). At month 6, SARS-CoV-2 polymerase chain reaction was negative in all except 1 patient. Of the 42 patients, 30 (71.4%) were seropositive (IgG> 1.4) at month 2 (Figure 1a). Among the 21 of 33 patients (63.6%) who were IgG-positive at month 2 and who had available sera at month 6, 12 (57.1%) remained positive (index ≥1.4) at month 6, while 9 (42.9%) had negative or equivocal results. Overall, 21 of 33 patients (63.6%) had an IgG index <1.4 at month 6 (Figure 1a), including 14 of 24 patients (58.3%) and 4 of 7 (57.1%) who, respectively, required hospitalization and ICU stay at the time of the COVID-19 episode. IgG index decreased between months 2 and 6 in all patients including in patients requiring hospitalization or ICU stay. Median IgG index fell from 3.6 (IQR: 1.3–5.1) at month 2 to 0.7 (IQR: 0.1–2.0) at month 6 (Figure 1b and c). Median decrease was 80.3% (IQR: 60.8%–83.3%; P < 0.0001). No patient relapsed from COVID-19 infection. At month 6, there was no correlation between IgG index and initial disease severity (P = 0.65), post-transplantation delay (P = 0.99), or induction therapy by anti-thymocyte globulins (P = 0.77). In conclusion, this is the first study assessing the anti–SARS-CoV2-IgG trajectory over a period of 6 months after disease onset in KTRs. Our results confirm that most KTRs develop specific antibodies against SARS-CoV-2.1Prendecki M. Clarke C. Gleeson S. et al.Detection of SARS-CoV-2 antibodies in kidney transplant recipients.J Am Soc Nephrol. 2020; 31: 2753-2756Crossref PubMed Scopus (28) Google Scholar However, antibody levels rapidly decrease in all patients and more than 60% had negative or equivocal IgG results at month 6. Interestingly, antibodies turned also negative or equivocal in patients with severe forms. Data about anti–SARS-CoV-2 antibodies' duration in the general population are controversial.3Dan J.M. Mateus J. Kato Y. et al.Immunological memory to SARS-CoV-2 assessed for greater than six months after infection.http://biorxiv.org/lookup/doi/10.1101/2020.11.15.383323Google Scholar,4Bölke E. Matuschek C. Fischer J.C. Loss of anti–SARS-CoV-2 antibodies in mild Covid-19.N Engl J Med. 2020; 383: 1694-1698Crossref PubMed Scopus (15) Google Scholar However, antibodies' decline had been mainly described in mild disease forms.4Bölke E. Matuschek C. Fischer J.C. Loss of anti–SARS-CoV-2 antibodies in mild Covid-19.N Engl J Med. 2020; 383: 1694-1698Crossref PubMed Scopus (15) Google Scholar,5Patel M.M. Thornburg N.J. Stubblefield W.B. et al.Change in antibodies to SARS-CoV-2 over 60 days among health care personnel in Nashville, Tennessee.JAMA. 2020; 324: 1781-1782Crossref PubMed Scopus (100) Google Scholar Further studies are needed to assess long-term antibody response in KTRs and its potential correlation with COVID-19 reinfections or relapses, as well as the efficacy of the vaccine in this population. Disparity between levels of anti-RBD IgG and anti-nucleocapsid protein IgG antibodies in COVID-19–recovered patients who received a kidney transplantKidney InternationalVol. 100Issue 1PreviewWe read with great interest the study by Chavarot et al.,1 which demonstrated that anti–severe acute respiratory syndrome coronavirus disease 2 (SARS-CoV-2) anti-nucleocapsid (N) protein IgG declines rapidly following SARS-CoV-2 infection in patients who received a kidney transplant (KTx-pts), independent of illness severity, but did not address the dynamic interplay with IgG antibodies against the spike protein–receptor binding domain (spike-RBD). Full-Text PDF