tbuckley/cabot-search huggingface dataset:
Orchestrated cellular, biochemical, and biomechanical optimizations endow platelet-rich plasma-based engineered cartilage with structural and biomechanical recovery
Authors: Ketao Wang, Ji Li, Yuxing Wang, Yaqiang Wang, Yuanyuan Qin, Fei Yang, Mingzhu Zhang, Heng Zhu, Zhongli Li
Journal: Bioactive Materials (2021)

Recently, biomaterials for cartilage regeneration has been intensively investigated. However, the development of scaffolds that capture regenerated cartilage with biomechanical and structural recovery has rarely been reported. To address this challenge, platelet-rich plasma (PRP)-based cartilage constructs with a well-orchestrated symphony of cellular, biochemical and biomechanical elements were prepared by simultaneously employing chondrogenic progenitor cells (CPCs) as a cell source, optimizing platelet concentration, and adding an enzyme-ion activator. It was shown that this triple-optimized PRP + CPC construct possessed increased biomechanical properties and suitable biochemical signals. The following in vitro study demonstrated that the triple-optimized PRP + CPC constructs generated cartilage-like tissue with higher expression levels of chondrogenic-specific markers, more deposition of cartilage-specific extracellular matrix (ECM), and greater biomechanical values than those of the other constructs. Twelve weeks after the construct was implanted in a cartilage defect in vivo, histological analysis, qPCR, and biomechanical tests collectively showed that the triple-optimized constructs yielded a more chondrocyte-like cell phenotype with a higher synthesis of Col-II and aggrecan. More importantly, the triple-optimized constructs facilitated cartilage regeneration with better biomechanical recovery than that of the other constructs. These results demonstrate the efficacy of the triple-optimization strategy and highlight the simplicity and potency of this PRP + CPC construct for cartilage regeneration.
A HIGH-PERFORMANCE MACHINE LEARNING MODEL TO PREDICT 90-DAY ACUTE HEART FAILURE READMISSION AND DEATH IN HEART FAILURE WITH PRESERVED EJECTION FRACTION
Authors: Jaeyoung Park, Farnaz Babaie Sarijaloo, Catarina Canha, Xiang Zhong, Anita Wokhlu
Journal: Journal of the American College of Cardiology (2021)
NGS mismapping confounds the clinical interpretation of the <i>PRSS1</i> p.Ala16Val (c.47C&gt;T) variant in chronic pancreatitis
Authors: Emmanuelle Génin, David N Cooper, Emmanuelle Masson, Claude Férec, Jian-Min Chen
Journal: Gut (2021)

M a s s o n, E m m a n u ell e, F é r e c, Cl a u d e a n d C h e n, Jia n-Mi n 2 0 2 2 .
ALPROSTADIL VERSUS ISOSORBIDE DINITRATE IN AMELIORATING ANGINA EPISODES IN PATIENTS WITH CORONARY SLOW FLOW PHENOMENON
Authors: Weifeng Zhang, Jinjie Dai, Yue Jiang, Xiaowen Zheng, Ke Xu, Xiaoxiao Yang, Xiaolei Wang, Lan Shen, Xingbiao Qiu, Linghong Shen, Ben He
Journal: Journal of the American College of Cardiology (2021)
COMPARATIVE EFFICACY AND SAFETY OF TICAGRELOR AND ASPIRIN DUAL ANTIPLATELET THERAPY VS TICAGRELOR MONOTHERAPY IN PATIENTS UNDERGOING PCI : A HEAD TO HEAD META ANALYSIS
Authors: Aminah Abdul Razzack, Faiza Ahmed, Faris Pacha, Sri Mandava, Nirmit Patel, Priyanka Panday, Syed Adeel Hassan, Farah Yasmin
Journal: Journal of the American College of Cardiology (2021)
INFLUENCE OF HEALTH INSURANCE ON LONG-TERM ADHERENCE TO STATINS AND BETA-BLOCKERS AFTER ACUTE CORONARY SYNDROMES
Authors: Jose Carlos Nicolau, Rocío Salsoso, Talia Dalcoquio, Paulo Genestreti, Andre Franci, Aline Ferrari, Adriadne Bertolin, Livia Lara, Marcela Juliasz, Cesar Pereira, Felipe Lima, Luciano Baracioli, Roberto Giraldez, Remo Furtado
Journal: Journal of the American College of Cardiology (2021)
Promoting musculoskeletal system soft tissue regeneration by biomaterial-mediated modulation of macrophage polarization
Authors: Jinchun Ye, Chang Xie, Canlong Wang, Jiayun Huang, Zi Yin, Boon Chin Heng, Xiao Chen, Weiliang Shen
Journal: Bioactive Materials (2021)

Musculoskeletal disorders are common in clinical practice. Repairing critical-sized defects in musculoskeletal systems remains a challenge for researchers and surgeons, requiring the application of tissue engineering biomaterials. Successful application depends on the response of the host tissue to the biomaterial and specific healing process of each anatomical structure. The commonly-held view is that biomaterials should be biocompatible to minimize local host immune response. However, a growing number of studies have shown that active modulation of the immune cells, particularly macrophages, via biomaterials is an effective way to control immune response and promote tissue regeneration as well as biomaterial integration. Therefore, we critically review the role of macrophages in the repair of injured musculoskeletal system soft tissues, which have relatively poor regenerative capacities, as well as discuss further enhancement of target tissue regeneration via modulation of macrophage polarization by biomaterial-mediated immunomodulation (biomaterial properties and delivery systems). This active regulation approach rather than passive-evade strategy maximizes the potential of biomaterials to promote musculoskeletal system soft tissue regeneration and provides alternative therapeutic options for repairing critical-sized defects.
MAGNETIC RESONANCE IMAGING BASED AORTIC PULSE WAVE VELOCITY NORMAL AND REFERENCE VALUES IN THE MIDDLE-AGED GENERAL POPULATION
Authors: Max Johan Pieter Van Hout, Ilona A. Dekkers, Jos J. Westenberg, Martin Schalij, Renée de Mutsert, Frits Rosendaal, Albert De Roos, Johan Jukema, Arthur Scholte, Hildo J. Lamb
Journal: Journal of the American College of Cardiology (2021)
Pals1 prevents Rac1-dependent colorectal cancer cell metastasis by inhibiting Arf6
Authors: Simona Mareike Lüttgenau, Christin Emming, Thomas Wagner, Julia Harms, Justine Guske, Katrin Weber, Ute Neugebauer, Rita Schröter, Olga Panichkina, Zoltán Pethő, Florian Weber, Albrecht Schwab, Anja Kathrin Wege, Pavel Nedvetsky, Michael P. Krahn
Journal: Molecular Cancer (2021)

Loss of apical-basal polarity and downregulation of cell-cell contacts is a critical step during the pathogenesis of cancer. Both processes are regulated by the scaffolding protein Pals1, however, it is unclear whether the expression of Pals1 is affected in cancer cells and whether Pals1 is implicated in the pathogenesis of the disease.Using mRNA expression data and immunostainings of cancer specimen, we show that Pals1 is frequently downregulated in colorectal cancer, correlating with poorer survival of patients. We further found that Pals1 prevents cancer cell metastasis by controlling Rac1-dependent cell migration through inhibition of Arf6, which is independent of the canonical binding partners of Pals1. Loss of Pals1 in colorectal cancer cells results in increased Arf6 and Rac1 activity, enhanced cell migration and invasion in vitro and increased metastasis of transplanted tumor cells in mice. Thus, our data reveal a new function of Pals1 as a key inhibitor of cell migration and metastasis of colorectal cancer cells. Notably, this new function is independent of the known role of Pals1 in tight junction formation and apical-basal polarity.
Circular RNA circZbtb20 maintains ILC3 homeostasis and function via Alkbh5-dependent m6A demethylation of Nr4a1 mRNA
Authors: Benyu Liu, Nian Liu, Xiaoxiao Zhu, Liuliu Yang, Buqing Ye, Huimu Li, Pingping Zhu, Tiankun Lu, Yong Tian, Zusen Fan
Journal: Cellular and Molecular Immunology (2021)
Sentinel Lymph Node Concept in High–Risk Profile Endometrial Cancer
Authors: Jan Persson, Celine Lönnerfors, Henrik Falconer
Journal: JAMA Surgery (2021)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
CORONARY VOLUME TO LEFT VENTRICULAR MASS RATIO DERIVED FROM CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY IN PATIENTS WITH DIABETES MELLITUS
Authors: Jurrien Kuneman, Mohammed El Mahdiui, Alexander van Rosendael, Manesh Patel, Bjarne Norgaard, Tim Fairbairn, Koen Nieman, Takashi Akasaka, Daniel Berman, Lynne Koweek, Gianluca Pontone, Tomohiro Kawasaki, Niels P. Ronnow Sand, Jesper Jensen, Tetsuya Amano, Michael Poon, Kristian A. Ovrehus, Jeroen Sonck, Mark Rabbat, Bernard De Bruyne, Campbell Rogers, Hitoshi Matsuo, Jeroen Bax, Jonathon Leipsic, Juhani Knuuti
Journal: Journal of the American College of Cardiology (2021)
REAL WORLD ANALYSIS OF OUTCOMES OF REMOTE PULMONARY ARTERY HEMODYNAMIC MONITORING IN HEART FAILURE PATIENTS OF DIFFERENT AGE GROUPS
Authors: Mansi Oberoi, Muhammad Hamza Saad Shaukat, Smitha Narayana Gowda, Adam Stys, Tomasz Stys, Marian Petrasko, Naveen Rajpurohit, Deborah Vicinsky, Orvar Jonsson
Journal: Journal of the American College of Cardiology (2021)
Promoter G-quadruplex folding precedes transcription and is controlled by chromatin
Authors: Jiazhen Shen, Dhaval Varshney, Angela Simeone, Xiaoyun Zhang, Santosh Adhikari, David Tannahill, Shankar Balasubramanian
Journal: Genome biology (2021)

Abstract Background Four-stranded G-quadruplexes (G4s) are DNA secondary structures in the human genome that are primarily found in active promoters associated with elevated transcription. Here, we explore the relationship between the folding of promoter G4s, transcription and chromatin state. Results Transcriptional inhibition by DRB or by triptolide reveals that promoter G4 formation, as assessed by G4 ChIP-seq, does not depend on transcriptional activity. We then show that chromatin compaction can lead to loss of promoter G4s and is accompanied by a corresponding loss of RNA polymerase II (Pol II), thus establishing a link between G4 formation and chromatin accessibility. Furthermore, pre-treatment of cells with a G4-stabilising ligand mitigates the loss of Pol II at promoters induced by chromatin compaction. Conclusions Overall, our findings show that G4 folding is coupled to the establishment of accessible chromatin and does not require active transcription.
A RARE CASE OF PERSISTENT THEBESIAN VEIN SYNDROME MASQUERADING CONSTRICTIVE PERICARDITIS
Authors: Dakshin Gangadharamurthy, Mandar Jagtap, Allan Klein, Ankur Kalra, Natesa Pandian, Muhammad Furqan, Saurabh Malhotra, Brian Borkowski, Keith Anderson, Michael McDonald, Mehul Patel
Journal: Journal of the American College of Cardiology (2021)
OUTCOMES OF URGENT VERSUS NON-URGENT TRANSCATHETER EDGE TO EDGE MITRAL VALVE REPAIR USING THE MITRACLIP DEVICE - AN INSIGHT FROM THE NATIONAL INPATIENT SAMPLE DATABASE
Authors: Venkata Missula, Yasser Al-Khadra, Basma Al-bast, Prashanth Singanallur, Radhika Deshpande, Raad Al Tamimi, Manasik Abdu, Nour Albast, Abdul Moiz Hafiz
Journal: Journal of the American College of Cardiology (2021)
STATIN ELIGIBILITY PER CHOLESTEROL GUIDELINES PRIOR TO STEMI IN PATIENTS IN INDIA - THE NORTH INDIA ST-ELEVATION MYOCARDIAL INFARCTION REGISTRY (NORIN-STEMI)
Authors: Sameer Arora, Arman Qamar, Puneet Gupta, Michael Hendrickson, Avinainder Singh, Muthiah Vaduganathan, Ambarish Pandey, Ankit Bansal, Vishal Batra, Saibal Mukhopadhyay, Bhawna Mahajan, MP Girish, Jamal Yusuf, Prashant Kaul, Deepak Bhatt, Mohit Gupta
Journal: Journal of the American College of Cardiology (2021)
Human Biology and Racial Welfare.
Journal: JAMA (1930)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
THE CURIOUS DISCOVERY OF A NEW INTRACAVITARY RIGHT ATRIAL CONTINUOUS DOPPLER FLOW SIGNAL; A DIAGNOSTIC AND MANAGEMENT DILEMMA
Authors: Umair Tariq, Pallavi Satuluri, Sagar Vadhar, Tarick Sheikh, Rahul Gupta, Shailendra Singh, Bruce Feldman, Raymond Singer
Journal: Journal of the American College of Cardiology (2021)
Metagenomic analysis of mother-infant gut microbiome reveals global distinct and shared microbial signatures
Authors: Shaopu Wang, Shuqin Zeng, Muireann Egan, Paul Cherry, Conall Strain, Emilene Morais, Patrick Boyaval, C. Anthony Ryan, Eugene Dempsey, R. Paul Ross, Catherine Stanton
Journal: Gut Microbes (2021)

Emerging evidence indicates maternal microbiota as one major reservoir for pioneering microbes in infants. However, the global distinct and identical features of mother–infant gut microbiota at various taxonomic resolutions and metabolic functions across cohorts and potential of infant microbial prediction based on their paired mother's gut microbiota remain unclear. Here, we analyzed 376 mother–infant dyads (468 mother and 1024 infant samples) of eight studies from six countries and observed higher diversity at species and strain levels in maternal gut microbiota but not their metabolic functions. A number of 290 species were shared in at least one mother–infant dyad, with 26 species (five at strain level) observed across cohorts. The profile of mother–infant shared species and strains was further influenced by delivery mode and feeding regimen. The mother-sourced species in infants exhibited similar strain heterogeneity but more metabolic functions compared to other-sourced species, suggesting the comparable stability and fitness of shared and non-shared species and the potential role of shared species in the early gut microbial community, respectively. Predictive models showed moderate performance accuracy for shared species and strains occurrences in infants. These generalized mother–infant shared species and strains may be considered as the primary targets for future work toward infant microbiome development and probiotics exploration.
Decoding Cell Death: From a Veritable Library of Babel to<i>Vade Mecum</i>?
Authors: Lindsey D. Hughes, Yaqiu Wang, Alexandre P. Meli, Carla V. Rothlin, Sourav Ghosh
Journal: Annual Review of Immunology (2021)

Programmed cell death (PCD) is a requisite feature of development and homeostasis but can also be indicative of infections, injuries, and pathologies. In concordance with these heterogeneous contexts, an array of disparate effector responses occur downstream of cell death and its clearance—spanning tissue morphogenesis, homeostatic turnover, host defense, active dampening of inflammation, and tissue repair. This raises a fundamental question of how a single contextually appropriate response ensues after an event of PCD. To explore how complex inputs may together tailor the specificity of the resulting effector response, here we consider ( a) the varying contexts during which different cell death modalities are observed, ( b) the nature of the information that can be passed on by cell corpses, and ( c) the ways by which efferocyte populations synthesize signals from dying cells with those from the surrounding microenvironment.
A NOVEL RISK-SCORE MODEL OF IN-HOSPITAL ACUTE KIDNEY INJURY BASED ON COMBINED BLOOD VARIABLES IN PATIENTS WITH ST-SEGMENT ELEVATION MYOCARDIAL INFARCTION
Authors: Yuhei Goriki, Atsushi Tanaka, Kensaku Nishihira, Atsushi Kawaguchi, Masahiro Natsuaki, Nehiro Kuriyama, Yoshisato Shibata, Koichi Node
Journal: Journal of the American College of Cardiology (2021)
PREVALENCE OF FAMILIAL HYPERCHOLESTEROLEMIA IN MYOCARDIAL INFARCTION IN THE YOUNG: PERSPECTIVES FROM THE NATIONAL INPATIENT SAMPLE 2016-2017
Authors: Daniel Antwi-Amoabeng, Mark B. Ulanja, Joban Ghuman, Bryce D. Beutler, Jasmine Ghuman, Nso Nso, Nageshwara Gullapalli, T. David Gbadebo
Journal: Journal of the American College of Cardiology (2021)
A RARE COMPLICATION OF A RARE SYNDROME: PROFOUND CARDIOVASCULAR COLLAPSE AND RIGHT LEG ISCHEMIA REQUIRING ECMO FROM EXTREME GASTRIC DISTENSION AND ACUTE ABDOMINAL COMPARTMENT SYNDROME
Authors: Ahmed Nagy, Nouran Sorour, Mary E. Huerter, Ricardo Bello, Colleen Harrington
Journal: Journal of the American College of Cardiology (2021)
TEMPORAL TRENDS OF INVASIVE PHYSIOLOGIC ASSESSMENT OF CORONARY ARTERY STENOSIS SEVERITY: INSIGHTS FROM A QUATERNARY-CARE CENTER IN THE UNITED STATES
Authors: Shawn Gogia, Madison Edens, Khady Fall, Denizhan Ozdemir, Hussein Rahim, Jeffrey Moses, Dimitrios Karmpaliotis, Ajay Kirtane, Ori Ben-Yehuda, Bjorn Redfors, Martin Leon, Mitsuaki Matsumura, Allen Jeremias, Akiko Maehara, Gary Mintz, Ziad Ali
Journal: Journal of the American College of Cardiology (2021)
A human-airway-on-a-chip for the rapid identification of candidate antiviral therapeutics and prophylactics
Authors: Longlong Si, Haiqing Bai, Melissa Rodas, Wuji Cao, Crystal Yuri Oh, Amanda Jiang, Rasmus Moller, Daisy Hoagland, Kohei Oishi, Shu Horiuchi, Skyler Uhl, Daniel Blanco-Melo, Randy A. Albrecht, Wen-Chun Liu, Tristan Jordan, Benjamin E. Nilsson-Payant, Ilona Golynker, Justin Frere, James Logue, Robert Haupt, Marisa McGrath, Stuart Weston, Tian Zhang, Roberto Plebani, Mercy Soong, Atiq Nurani, Seong Min Kim, Danni Y. Zhu, Kambez H. Benam, Girija Goyal, Sarah E. Gilpin, Rachelle Prantil-Baun, Steven P. Gygi, Rani K. Powers, Kenneth E. Carlson, Matthew Frieman, Benjamin R. tenOever, Donald E. Ingber
Journal: Nature Biomedical Engineering (2021)
PLAQUE BURDEN IN TWO ADOLESCENTS WITH HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA: TURNING BACK THE CLOCK WITH AGGRESSIVE LDL-C LOWERING
Authors: Nick Nurmohamed, Rens Reeskamp, Michiel Bom, Nils Planken, Roel Driessen, Pepijn Van Diemen, Irene Kuipers, Albert Wiegman, G Kees Hovingh
Journal: Journal of the American College of Cardiology (2021)
AIR POLLUTION AND ADVERSE CARDIAC REMODELLING IN PATIENTS WITH DILATED CARDIOMYOPATHY
Authors: Upasana Tayal, Daniela Fecht, Marc Chadeau-Hyam, Ruth Owen, John Gregson, Brian Halliday, Amrit Lota, John Gulliver, James Ware, Dudley Pennell, Frank Kelly, Anoop Shah, Mark Miller, David Newby, Sanjay Prasad
Journal: Journal of the American College of Cardiology (2021)
Covid-19: How can we keep the world’s doctors safe?
Authors: Fiona Godlee
Journal: BMJ (2021)

The world’s doctors have been at the forefront of the pandemic response. Already overstretched by workforce shortages, they have carried exhaustion, uncertainty, and risk, redeploying to unfamiliar specialties, learning at speed, and adopting different ways of working and new technology,1 while all the time fearing for their patients, their families, and themselves. For many there has been the added burden of betrayal by governments, whose decisions have caused avoidable harm through waste, corruption, and incompetence and through policies driven by ideology rather than evidence.23

Nothing shows this more starkly than the events unfolding in …
Abstract 15227: Egln1 Deficiency Induce Alveolar Macrophages Accumulation and Polarize Lung Interstitial Macrophages to a Pro-pah State Mediating Obliterative Pulmonary Vascular Remodeling and Severe PAH
Authors: Jingbo Dai, Zhiyu Dai, Mengqi Zhu, Daoyin Dong, Youyang Zhao
Journal: Circulation (2020)

Introduction: Previously we identified a mouse model (Tie2Cre-mediated disruption of Egln1 ) of Pulmonary arterial hypertension (PAH) with progressive obliterative vascular remodeling including vascular occlusion and plexiform-like lesions and right heart failure. Tie2Cre-meidated disruption of Egln1 in bone marrow contributes to the severity of PAH indicating the importance of the immune cells in the pathogenesis of PH. Hypothesis: Macrophage accumulation and polarization due to Egln1 deficiency contributes to obliterative pulmonary vascular remodeling and severe PAH in Egln1 Tie2Cre mice. Methods: Egln1 Tie2Cre mice with macrophage Fas-Induced apoptosis (MaFIA) transgene were generated (Egln1 Tie2Cre /MaFIA) and received AP20187 treatment to deplete the macrophages during the development of PAH afterwards the RVSP and RV/(LV+S) ratio were measured as the indicator of diseases severity. Flowcytometry and immunostaining were used to quantify different types of macrophages. Bulk RNA-seq and single cell RNA-seq were used to identify PAH-related pathway changes and population changes of lung macrophages in Egln1 Tie2Cre mice. BrdU staining was used to measure the smooth muscle cell (SMC) proliferation co-culturing with macrophages isolated from Egln1 Tie2Cre mice. Results: We observed that CD206+ Alternative Activated Macrophages (AAMs) are accumulated in the pulmonary vessel adventitia of IPAH patients as well as Egln1 Tie2Cre mice. These macrophages are Egln1 deficient and express elevated level of pro-PAH factors and thus promote SMC proliferation in vitro . Moreover, genetic depletion of total macrophages using the Egln1 Tie2Cre /MaFIA mouse model markedly inhibited obliterative pulmonary vascular remodeling and PAH. Single cell RNA-seq revealed that lung resident alveolar macrophages (Siglec-F+/Cd11c+) accumulated due to hyper-proliferation while recruited lung interstitial (Cx3cr1+/Aif1+) macrophages polarize to a pro-PAH state in Egln1 Tie2Cre mice. Conclusions: Egln1 deficiency in the HCs induced proliferation/accumulation of alveolar macrophages and polarized the recruited interstitial macrophages to AAM state which contributes to the vascular remodeling by secreting pro-PAH factors.