tbuckley/cabot-search huggingface dataset:
miR-7/TGF-β2 axis sustains acidic tumor microenvironment-induced lung cancer metastasis
Authors: Tao Su, Suchao Huang, Yanmin Zhang, Yajuan Guo, Shuwei Zhang, Jiaji Guan, Mingjing Meng, Linxin Liu, Caiyan Wang, Dihua Yu, Hiu-Yee Kwan, Zhiying Huang, Qiuju Huang, Elaine Lai-Han Leung, Ming Hu, Ying Wang, Zhongqiu Liu, Linlin Lu
Journal: Acta Pharmaceutica Sinica B (2021)

Acidosis, regardless of hypoxia involvement, is recognized as a chronic and harsh tumor microenvironment (TME) that educates malignant cells to thrive and metastasize. Although overwhelming evidence supports an acidic environment as a driver or ubiquitous hallmark of cancer progression, the unrevealed core mechanisms underlying the direct effect of acidification on tumorigenesis have hindered the discovery of novel therapeutic targets and clinical therapy. Here, chemical-induced and transgenic mouse models for colon, liver and lung cancer were established, respectively. miR-7 and TGF-β2 expressions were examined in clinical tissues (n = 184). RNA-seq, miRNA-seq, proteomics, biosynthesis analyses and functional studies were performed to validate the mechanisms involved in the acidic TME-induced lung cancer metastasis. Our data show that lung cancer is sensitive to the increased acidification of TME, and acidic TME-induced lung cancer metastasis via inhibition of miR-7-5p. TGF-β2 is a direct target of miR-7-5p. The reduced expression of miR-7-5p subsequently increases the expression of TGF-β2 which enhances the metastatic potential of the lung cancer. Indeed, overexpression of miR-7-5p reduces the acidic pH-enhanced lung cancer metastasis. Furthermore, the human lung tumor samples also show a reduced miR-7-5p expression but an elevated level of activated TGF-β2; the expressions of both miR-7-5p and TGF-β2 are correlated with patients' survival. We are the first to identify the role of the miR-7/TGF-β2 axis in acidic pH-enhanced lung cancer metastasis. Our study not only delineates how acidification directly affects tumorigenesis, but also suggests miR-7 is a novel reliable biomarker for acidic TME and a novel therapeutic target for non-small cell lung cancer (NSCLC) treatment. Our study opens an avenue to explore the pH-sensitive subcellular components as novel therapeutic targets for cancer treatment.
External Validation of a Widely Implemented Proprietary Sepsis Prediction Model in Hospitalized Patients
Authors: Andrew Wong, Erkin Otles, John P. Donnelly, Andrew Krumm, Jeffrey McCullough, Olivia DeTroyer-Cooley, Justin Pestrue, Marie Phillips, Judy Konye, Carleen Penoza, Muhammad Ghous, Karandeep Singh
Journal: JAMA Internal Medicine (2021)

<h3>Importance</h3> The Epic Sepsis Model (ESM), a proprietary sepsis prediction model, is implemented at hundreds of US hospitals. The ESM's ability to identify patients with sepsis has not been adequately evaluated despite widespread use. <h3>Objective</h3> To externally validate the ESM in the prediction of sepsis and evaluate its potential clinical value compared with usual care. <h3>Design, Setting, and Participants</h3> This retrospective cohort study was conducted among 27 697 patients aged 18 years or older admitted to Michigan Medicine, the academic health system of the University of Michigan, Ann Arbor, with 38 455 hospitalizations between December 6, 2018, and October 20, 2019. <h3>Exposure</h3> The ESM score, calculated every 15 minutes. <h3>Main Outcomes and Measures</h3> Sepsis, as defined by a composite of (1) the Centers for Disease Control and Prevention surveillance criteria and (2)<i>International Statistical Classification of Diseases and Related Health Problems, Tenth Revision</i>diagnostic codes accompanied by 2 systemic inflammatory response syndrome criteria and 1 organ dysfunction criterion within 6 hours of one another. Model discrimination was assessed using the area under the receiver operating characteristic curve at the hospitalization level and with prediction horizons of 4, 8, 12, and 24 hours. Model calibration was evaluated with calibration plots. The potential clinical benefit associated with the ESM was assessed by evaluating the added benefit of the ESM score compared with contemporary clinical practice (based on timely administration of antibiotics). Alert fatigue was evaluated by comparing the clinical value of different alerting strategies. <h3>Results</h3> We identified 27 697 patients who had 38 455 hospitalizations (21 904 women [57%]; median age, 56 years [interquartile range, 35-69 years]) meeting inclusion criteria, of whom sepsis occurred in 2552 (7%). The ESM had a hospitalization-level area under the receiver operating characteristic curve of 0.63 (95% CI, 0.62-0.64). The ESM identified 183 of 2552 patients with sepsis (7%) who did not receive timely administration of antibiotics, highlighting the low sensitivity of the ESM in comparison with contemporary clinical practice. The ESM also did not identify 1709 patients with sepsis (67%) despite generating alerts for an ESM score of 6 or higher for 6971 of all 38 455 hospitalized patients (18%), thus creating a large burden of alert fatigue. <h3>Conclusions and Relevance</h3> This external validation cohort study suggests that the ESM has poor discrimination and calibration in predicting the onset of sepsis. The widespread adoption of the ESM despite its poor performance raises fundamental concerns about sepsis management on a national level.
Functional human IgA targets a conserved site on malaria sporozoites
Authors: Joshua Tan, Hyeseon Cho, Tossapol Pholcharee, Lais S. Pereira, Safiatou Doumbo, Didier Doumtabe, Barbara J. Flynn, Arne Schön, Sachie Kanatani, Samantha O. Aylor, David Oyen, Rachel Vistein, Lawrence Wang, Marlon Dillon, Jeff Skinner, Mary Peterson, Shanping Li, Azza H. Idris, Alvaro Molina-Cruz, Ming Zhao, Lisa Renee Olano, Patricia J. Lee, Alison Roth, Photini Sinnis, Carolina Barillas-Mury, Kassoum Kayentao, Aissata Ongoiba, Joseph R. Francica, Boubacar Traore, Ian A. Wilson, Robert A. Seder, Peter D. Crompton
Journal: Science Translational Medicine (2021)

A functional human IgA response to malaria targets a conserved epitope in the N terminus of the Plasmodium falciparum circumsporozoite protein.
Investigation and management of severe thrombocytopaenia in a patient with cavitating lung disease
Authors: Aya M Abbas, Kate Brackenborough, Sarah Menzies, John Willan, Jasmine Ming Gan, Safoora Rehman
Journal: Thorax (2021)

A 44-year-old Polish man attended the emergency department complaining of cough and pleuritic chest pain. He had a fever of 38.6°C and his oxygen saturation (SpO2) was 97%. His medical history included childhood asthma. He took no medication or over-the-counter remedies. He was a smoker with a 60-pack year history and consumed 200 units of alcohol a week for 16 years, but reported he stopped drinking 1 month ago. He migrated from Poland 15 years ago and worked as a manual labourer. He denied any recent foreign travel. A chest X-ray (CXR) showed a cavitating lung lesion, and a subsequent urgent CT scan of the chest performed to rule out malignancy demonstrated a cavitating consolidation in the left posterior upper lobe and consolidation of the right lateral middle lobe (figures 1–4). Cavitating pneumonia in those with alcohol excess usually occurs in the posterior segments of the upper lobes. He was prescribed a 1-week course of co-amoxiclav for suspected bacterial pneumonia and was referred to the respiratory outpatient clinic to investigate these lesions further. At that time, his full blood count was normal.



Figure 1 
Coronal CT chest image showing cavitating consolidation in the left posterior upper lobe.





Figure 2 
Coronal CT chest image showing consolidation in the right lateral middle lobe.





Figure 3 
Axial CT chest image showing cavitating consolidation in the left posterior upper lobe.





Figure 4 
Axial CT chest image showing consolidation in the right lateral middle lobe.



We met this patient 1 month later when he re-presented to the emergency department, with a 1-day history of moderate-volume haemoptysis and epistaxis on a background of left lateral chest pain, dry cough and exertional dyspnoea. He was apyrexic and his SpO2 was 95% at rest. He reported loss of appetite and 40 kg weight loss over the past year. Weighing 93 kg on admission with new onset lethargy …
Immunogenicity of the BNT162b2 COVID-19 mRNA vaccine and early clinical outcomes in patients with haematological malignancies in Lithuania: a national prospective cohort study
Authors: Kazimieras Maneikis, Karolis Šablauskas, Ugnė Ringelevičiūtė, Vilmantė Vaitekėnaitė, Rita Čekauskienė, Lina Kryžauskaitė, Daniel Naumovas, Valdas Banys, Valdas Pečeliūnas, Tumas Beinortas, Laimonas Griškevičius
Journal: The Lancet Haematology (2021)
Abstract 216: Intra-myocardial Injection of a Novel Thermosensitive Hydrogel Inhibits Post-infarct Heart Failure in Rats
Authors: Ying Wen, Xiao-yan Li, Ze-yong Li, Meng-long Wang, Xian-zheng Zhang, Xue-jun Jiang
Journal: Circulation Research (2016)

Intra-myocardial injection of hydrogel after myocardial infarction (MI) has been confirmed for preventing post-infarct ventricular remodeling (VR) in numerous studies. Meanwhile, VR or existed heart failure (HF) symptoms of patients with MI was improved after intra-myocardial injection of hydrogels in recent clinical trials. However, whether the injection of hydrogel immediately after MI can prevent post-infarct HF in a long follow-up period is unclear. In present study, MI was induced by permanent coronary artery ligation in SD rats. Immediately, 100μl Dex-PCL-HEMA/ PNIPAAm (DPHP) hydrogel solution was injected into the infarct border myocardium of experimental rats, while phosphate-buffered saline (PBS) was employed in control group. Twelve weeks later, compared with PBS group (n=11), echocardiography and hemodynamics showed left ventricle dilatation was attenuated (end-diastolic diameter 9.30±0.73 mm vs 10.74±0.57 mm, p &lt;0.01) and cardiac function was improved (+dp/dt 12351±1158 mmHg/s vs 9488±695 mmHg/s, p &lt;0.01) in hydrogel group (n=11). Serum brain natriuretic peptide (107.74±18.21 pg/ml vs 132.17±20.62 pg/ml, p &lt;0.05) and cardiac aldosterone production of hydrogel group was suppressed, and so did pulmonary congestion. Histological analysis indicated thicker scar, smaller infarct size, diminished cardiac fibrosis (collagen volume fraction 21.74±3.13% vs 33.38±3.08%, p &lt;0.01) and hypertrophy in hydrogel group. Higher Col3α1 mRNA (27.32±0.71 vs 14.29±1.37, p &lt;0.01) in infarct zone but lower Col3α1 mRNA (0.97±0.12 vs 9.06±1.04, p &lt;0.01) and ANP mRNA (10.03±1.49 vs 24.74±2.40, p &lt;0.01) in non-infarct zone (NIZ) were detected by RT-PCR in hydrogel group. These data proves that the intra-myocardial injection of DPHP hydrogel immediately after MI can improve cardiac function including diastolic function, decrease infarct size, and enhance fibrotic healing in the infarct area. On the other hand, this treatment suppresses reactive fibrosis and hypertrophy in NIZ and thus delays post-infarct VR and prevents HF. DPHP hydrogel implantation immediately after MI is an effective strategy to prevent abnormal cardiac remodeling and have a long term beneficial effect even after it has been biodegraded.
Obesity, Adiposity, and Risk of Symptomatic Gallstone Disease According to Genetic Susceptibility
Authors: Junghyun Lim, Janine Wirth, Kana Wu, Edward Giovannucci, Peter Kraft, Constance Turman, Mingyang Song, Manol Jovani, Andrew T. Chan, Amit D. Joshi
Journal: Clinical Gastroenterology and Hepatology (2021)
Combined Clinical and Gene Expression Score Identifies Follicular Lymphoma Patients with High Risk of Transformation in the Rituximab Era
Authors: Chloe Beate Steen, Ellen Leich, June Helen Myklebust, Sandra Lockmer, Jillian Frances Wise, Björn Engelbrekt Wahlin, Bjørn Østenstad, Knut Liestol, Eva Kimby, Andreas Rosenwald, Erlend Bremertun Smeland, Harald Holte, Ole Christian Lingjærde, Marianne Brodtkorb
Journal: Blood (2017)
Abstract 15963: Where the Fontan Flows: Longitudinal Trends in Flow Through the Palliated Single Ventricle Circulation
Authors: Reena M Ghosh, Kevin K Whitehead, Matthew A Harris, Sara Partington, David M Biko, Mark A Fogel
Journal: Circulation (2019)

Background: In the past 2 decades patients living with Fontan physiology have had improved life expectancy but continued high rates of morbidity. Alterations in flow equilibrium within this circula...
Abstract SY02-02: Understanding evolution of metastatic disease in renal cancer and melanoma
Authors: Samra Turajlic
Journal: Cancer Research (2021)

Abstract While our knowledge of the evolutionary features of primary tumors has grown exponentially over the last few years our understanding of evolution of metastases is more limited and many open questions remain, regarding the timing of metastatic dissemination, the acquisition of metastatic competence and the underpinning of the variety of metastatic phenotypes observed in the clinic from latent metastases to solitary and oligo-metastases to widespread metastases. We have leveraged unique translational protocols that allow interrogation of tumor evolution from the earliest stages of diseases to death to address some of these questions. In the context of renal cell cancers we show that the mode of evolution at the primary tumor site determines the tempo and distribution of systemic metastases; that the features that distinguish metastasis-competent clones included genome instability and aneuploidy; and that metastatic competence often emerges in the centre of the primary tumor. In the context of melanoma we observe a wide spectrum of metastatic seeding that includes organ-specific metastatic clones, monophyletic and polyphyletic seeding and polyclonal mixing at metastatic sites. We observe progressive increase in aneuploidy and occurrence of whole genome doubling as melanoma metastases progress and resist treatment suggesting this as a a mechanism of immune-evasion and treatment resistance. Citation Format: Samra Turajlic. Understanding evolution of metastatic disease in renal cancer and melanoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr SY02-02.
Abstract 15323: Par2 Deficiency Protects the Heart Against Cvb3-Induced Myocarditis Via Upregulation of Toll-Like Receptor-3 Mediated INFβ Expression
Authors: Ursula Rauch, Alice Weithaeuser, Karin Klingel, Heinz-Peter Schultheiss
Journal: Circulation (2011)

Introduction: The protease activated receptor 2 (PAR2) is activated by serine proteases, such as the complex of tissue factor (TF)/ factor VII (FVII) and factor X (FX). Its proteolytic cleavage lea...
Corrigendum to “Psychological interventions to prevent the onset of depressive disorders: A meta-analysis of randomized controlled trials” [Clinical Psychology Review 83 (2021) 101955]
Authors: Pim Cuijpers, Blanca S. Pineda, Soledad Quero, Eirini Karyotaki, Sascha Y. Struijs, Caroline A. Figueroa, Jazmin A. Llamas, Toshi A. Furukawa, Ricardo F. Muñoz
Journal: Clinical Psychology Review (2021)
The impact of obesity and body weight on the outcome of patients with relapsed/refractory large B-cell lymphoma treated with axicabtagene ciloleucel
Authors: Kitsada Wudhikarn, Radhika Bansal, Arushi Khurana, Matthew A. Hathcock, N. Nora Bennani, Jonas Paludo, Jose C. Villasboas, Yucai Wang, Patrick B. Johnston, Stephen M. Ansell, Yi Lin
Journal: Blood Cancer Journal (2021)

The impact of obesity and body weight
We Can Heal From Hate Crimes by Practicing Solidarity
Authors: Christopher Cai
Journal: JAMA Internal Medicine (2021)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
Abstract 9556: A Risk Prediction Model for Hemorrhagic Events in Elderly Patients With Coronary Artery Disease and Malignant Gastrointestinal Tumors
Authors: Nandi Bao, Yabin Wang, Ran Zhang, Hebin Che, Huitao Wu, Wenwen Meng, Jiaxin Miao, Danyang Li, Wanguo Xue, Li Fan, Feng Cao
Journal: Circulation (2019)

Introduction: Elderly patients with coronary heart disease and malignant digestive tract tumor have a high incidence of bleeding events. In this study, a risk prediction model was established based...
若年男性に発生した,非典型的な画像所見を呈した巨大fibrolamellar carcinomaの1切除例
Authors: 松本尊嗣, 青木琢, 鈴木隆志, 清水崇行, 谷圭吾, Park Kyung Hwa, 白木孝之, 森昭三, 礒幸博, 加藤正人, 大日方謙介, 黒田一, 石塚満, 今井康雄, 窪田敬一
Journal: Liver Cancer (2018)
Long-Term Outcomes Following Myocardial Infarction in Young Adult Survivors of Hodgkin Lymphoma
Authors: Sanjay Divakaran, David W. Biery, Adam N. Berman, Avinainder Singh, Jon Hainer, Wanda Y. Wu, Marcelo F. Di Carli, Deepak L. Bhatt, Anju Nohria, Ron Blankstein
Journal: JACC CardioOncology (2021)
Association between Initial Treatment Strategy and Long-Term Survival in Pulmonary Arterial Hypertension
Authors: Athénaïs Boucly, Laurent Savale, Xavier Jaïs, Fabrice Bauer, Emmanuel Bergot, Laurent Bertoletti, Antoine Beurnier, Arnaud Bourdin, Hélène Bouvaist, Sophie Bulifon, Céline Chabanne, Ari Chaouat, Vincent Cottin, Claire Dauphin, Bruno Degano, Pascal De Groote, Nicolas Favrolt, Yuanchao Feng, Delphine Horeau-Langlard, Mitja Jevnikar, Etienne-Marie Jutant, Zhiying Liang, Pascal Magro, Pierre Mauran, Pamela Moceri, Jean-François Mornex, Sylvain Palat, Florence Parent, François Picard, Jérémie Pichon, Patrice Poubeau, Grégoire Prévot, Sébastien Renard, Martine Reynaud-Gaubert, Marianne Riou, Pascal Roblot, Olivier Sanchez, Andrei Seferian, Cécile Tromeur, Jason Weatherald, Gérald Simonneau, David Montani, Marc Humbert, Olivier Sitbon
Journal: American Journal of Respiratory and Critical Care Medicine (2021)

Rationale: The relationship between the initial treatment strategy and survival in pulmonary arterial hypertension (PAH) remains uncertain. Objectives: To evaluate the long-term survival of patients with PAH categorized according to the initial treatment strategy. Methods: A retrospective analysis of incident patients with idiopathic, heritable, or anorexigen-induced PAH enrolled in the French Pulmonary Hypertension Registry (January 2006 to December 2018) was conducted. Survival was assessed according to the initial strategy: monotherapy, dual therapy, or triple-combination therapy (two oral medications and a parenteral prostacyclin). Measurements and Main Results: Among 1,611 enrolled patients, 984 were initiated on monotherapy, 551 were initiated on dual therapy, and 76 were initiated on triple therapy. The triple-combination group was younger and had fewer comorbidities but had a higher mortality risk. The survival rate was higher with the use of triple therapy (91% at 5 yr) as compared with dual therapy or monotherapy (both 61% at 5 yr) (P < 0.001). Propensity score matching of age, sex, and pulmonary vascular resistance also showed significant differences between triple therapy and dual therapy (10-yr survival, 85% vs. 65%). In high-risk patients (n = 243), the survival rate was higher with triple therapy than with monotherapy or dual therapy, whereas there was no difference between monotherapy and double therapy. In intermediate-risk patients (n = 1,134), survival improved with an increasing number of therapies. In multivariable Cox regression, triple therapy was independently associated with a lower risk of death (hazard ratio, 0.29; 95% confidence interval, 0.11-0.80; P = 0.017). Among the 148 patients initiated on a parenteral prostacyclin, those on triple therapy had a higher survival rate than those on monotherapy or dual therapy. Conclusions: Initial triple-combination therapy that includes parenteral prostacyclin seems to be associated with a higher survival rate in PAH, particularly in the youngest high-risk patients.
Abstract 12327: Precision Medicine for the Trafficking Defective Mutation, p. N633S, in the Type 2 Long QT Syndrome-associated, KCNH2-encoded Kv11.1 Channel Using Patient-specific Re-engineered Cardiomyocytes
Authors: Samantha K Hamrick, CS John Kim, David J Tester, Michael J Ackerman
Journal: Circulation (2019)

Introduction: The KCNH2-encoded Kv11.1 (hERG) potassium channel is a critical regulator of the cardiomyocyte’s (CM) action potential duration (APD). The majority of type 2 long QT syndrome (LQT2) s...
Treatment of cystic fibrosis airway cells with CFTR modulators reverses aberrant mucus properties <i>via</i> hydration
Authors: Cameron B. Morrison, Kendall M. Shaffer, Kenza C. Araba, Matthew R. Markovetz, Jason A. Wykoff, Nancy L. Quinney, Shuyu Hao, Martial F. Delion, Alexis L. Flen, Lisa C. Morton, Jimmy Liao, David B. Hill, Mitchell L. Drumm, Wanda K. O'Neal, Mehmet Kesimer, Martina Gentzsch, Camille Ehre
Journal: European Respiratory Journal (2021)

Question Cystic fibrosis (CF) is characterised by the accumulation of viscous adherent mucus in the lungs. While several hypotheses invoke a direct relationship with cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction ( i.e. acidic airway surface liquid (ASL) pH, low bicarbonate (HCO 3 − ) concentration, airway dehydration), the dominant biochemical alteration of CF mucus remains unknown. Materials/methods We characterised a novel cell line (CFTR-KO Calu3 cells) and the responses of human bronchial epithelial (HBE) cells from subjects with G551D or F508del mutations to ivacaftor and elexacaftor-tezacaftor-ivacaftor. A spectrum of assays such as short-circuit currents, quantitative PCR, ASL pH, Western blotting, light scattering/refractometry (size-exclusion chromatography with inline multi-angle light scattering), scanning electron microscopy, percentage solids and particle tracking were performed to determine the impact of CFTR function on mucus properties. Results Loss of CFTR function in Calu3 cells resulted in ASL pH acidification and mucus hyperconcentration (dehydration). Modulation of CFTR in CF HBE cells did not affect ASL pH or mucin mRNA expression, but decreased mucus concentration, relaxed mucus network ultrastructure and improved mucus transport. In contrast with modulator-treated cells, a large fraction of airway mucins remained attached to naïve CF cells following short apical washes, as revealed by the use of reducing agents to remove residual mucus from the cell surfaces. Extended hydration, but not buffers alkalised with sodium hydroxide or HCO 3 − , normalised mucus recovery to modulator-treated cell levels. Conclusion These results indicate that airway dehydration, not acidic pH and/or low [HCO 3 − ], is responsible for abnormal mucus properties in CF airways and CFTR modulation predominantly restores normal mucin entanglement.
早期心室収縮誘発心筋症からの回復にもかかわらず持続性前不整脈神経リモデリング【JST・京大機械翻訳】
Authors: Y Tan Alex, Elharrif Khalid, Cardona-Guarache Ricardo, Mankad Pranav, Ayers Owen, Joslyn Martha, Das Anindita, Kaszala Karoly, Lin Shien-Fong, A Ellenbogen Kenneth, J Minisi Anthony, F Huizar Jose
Journal: Journal of the American College of Cardiology (2020)