tbuckley/cabot-search huggingface dataset:
Loss of CACNA2D2 expression in non-small cell lung cancer
Authors: George Xinarianos, Dimitrios Panutsopulos, Patricia Fielding, John R. Gosney, Demetrios A. Spandidos, Triantafillos Liloglou, John K. Field
Journal: Cancer Research (2006)

4195   Genetic events at chromosome 3p have been implicated in the development of non-small cell lung carcinoma (NSCLC). A number of known and putative tumour-suppressor genes reside within this region, including  CACNA2D2 . This is a candidate tumour-suppressor gene which is located at 3p21. Although  CACNA2D2 expression has previously been analysed in some lung cancer cell lines, its role in the development of primary NSCLC has yet to be elucidated. In this study, expression of the  CACNA2D2  gene was investigated in 55 paired (normal/tumour), surgically excised lung tissue samples from patients with NSCLC. Promoter methylation status and loss of heterozygosity (LOH) at 3p were investigated in 48 of these samples. expression levels of  CACNA2D2 were studied using comparative multiplex RT-PCR and subsequent analysis on an Agilent Bioanalyser. We have also developed a pyrosequencing assay on bisulphite treated DNA to examine the methylation status of part of the  CACNA2D2 promoter. Analysis was carried out on a PSQ96MA System. LOH was carried out using fluorescent microsatellite markers at three loci (D3S1289, D3S1300, and D3S1263) and analysis on a 377 ABI automated sequencer.  CACNA2D2  was underexpressed in 49 (89%) of 55 lung tumours compared to their adjacent normal tissue counterparts. The  CACNA2D2  promoter was hypermethylated, in at least one CpG site out of four examined, in 2 (4%) of 48 samples investigated. LOH was detected in 26 (67%) of 39, 26 (60%) of 43 and 23 (70%) of 33 informative cases in markers D3S1289, D3S1263 and D3S1300 respectively. Loss of  CACNA2D2  expression was more frequent in tumours with positive than tumours with negative nodes (Fisher’s exact, p=0.05). There was no association between  CACNA2D2  expression and histology, T stage, M status and differentiation. These data demonstrate that loss of  CACNA2D2  expression is a very frequent event in the development of NSCLC. Promoter hypermethylation of  CACNA2D2 is rare and doesn’t appear as a major mechanism accounting for this loss of expression. Other intrinsic or extrinsic factors may contribute to the transcriptional silencing of  CACNA2D2  in the molecular pathogenesis of NSCLC.  This research was funded by the Roy Castle Foundation UK.
Abstract 15235: A Pilot Study of Ac-SDKP (N-acetyl-seryl-aspartyl-lysyl-proline) and Galectin-3 Levels in Normal Pericardial Fluid and Tuberculous Pericardial Effusion: Implications for Pathogenesis and Prevention of Pericardial Constriction
Authors: Bongani M Mayosi, Mpiko Ntsekhe, Kerryn Matthews, Janine Wolske, Motasim Badri, Katalin Wilkinson, Robert J Wilkinson, Edward Sturrock
Journal: Circulation (2012)

Introduction Tuberculous pericarditis is associated with a high rate of progression to constrictive pericarditis. The cellular mediators and molecular mechanisms of post-inflammatory pericardial th...
A preliminary report on Crimean-Congo haemorrhagic fever in Turkey, March - June 2008
Authors: G R Yilmaz, T Buzgan, M A Torunoglu, A Safran, H Irmak, S Com, Y Uyar, A Carhan, E Ozkaya, M Ertek
Journal: Eurosurveillance (2008)

Crimean-Congo haemorrhagic fever (CCHF) is a disease caused by a virus belonging to Bunyaviridae family. CCHF virus isolation and/or disease have been reported from more than 30 countries in Africa, Asia, south-eastern Europe, and the Middle East [1]. The main transmission routes of the virus are tick-bite and contact with tissues, body fluids and blood of infected animals [1-4]. Nosocomial transmission is another important route of infection [1]. The incubation period is generally described as 1-3 days after tick-bite and 5-6 days after exposure to infected animal or human blood or body fluid, but it can be longer. Fever, chills, headache, fatigue and myalgia are the most common symptoms in the pre-haemorrhagic period. The disease progresses to haemorrhagic form in severe cases [1]. The fatality rate of disease is reported between 7.5-50% in hospitalised patients [4-7]. This wide range may due to phylogenetic variation of the virus, transmission route and different treatment facilities [4-7].
Abstract 3695: Obesity-mediated Vascular Injury Stimulates Progenitor Cell Mobilization
Authors: Salman Sher, Markus Porkert, Thomas R Zeigler, Margaret F Pedersen, Diane Sutcliffe, W Robert Taylor, Dean P Jones, Sandra B Dunbar, Craig Hooper, David Harrison, R Wayne Alexander, Arshed A Quyyumi
Journal: Circulation (2007)

Recent data suggest that bone marrow derived endothelial progenitor cells contribute to endothelial repair after damage. We investigated whether obesity modulates the number and function of progenitor cells, and whether lifestyle intervention with a healthy diet influences this effect. We hypothesized that obesity-mediated vascular injury stimulates progenitor cell mobilization. Methods Peripheral blood mononuclear cells were cultured on fibronectin plates for 7 days and the number of colony forming units (CFUs) with endothelial-type cells at their periphery were counted. 28 lean healthy subjects were compared with 30 overweight/obese age-and sex-matched non-smokers on a high saturated fat (&gt; 10%) American style diet. Twenty obese subjects were then randomized to receive either a Mediterranean-style (MED) diet (n=11) or the control regular American diet (n=9) for 2 months. Endothelium-dependent and -independent function was measured by brachial reactivity testing at baseline, 1 and 2 months. Results CFUs were significantly higher in overweight/obese compared to lean subjects (74±42 vs 23±10, p&lt;0.001), even after adjustment for other risk factors. When overweight/obese subjects were treated with a MED diet, CFUs reduced significantly, a change that was not observed in the controls (Table ). There was a simultaneous improvement in brachial flow-mediated vasodilation but not endothelium-independent function in the MED group. No changes were observed in controls(Table ). Conclusion Endothelial precursors are stimulated in obese compared to lean subjects. Dietary alteration to a MED diet resulted in improvement in endothelial function and reduced mobilization of endothelial precursors. These observations suggest that endothelial injury in obesity provokes a compensatory mobilization of endothelial progenitor cell population that may help repair damage to the endothelial lining.
Abstract 5052: Lowering of the Triglyceride/HDL Cholesterol Ratio Predicts the Benefit of Pioglitazone on Progression of Coronary Atherosclerosis in Diabetic Patients
Authors: S J Nicholls, O Bayturan, T Hu, S Kupfer, A Perez, Menon, A Askari, V S Srinivas, R W Nesto, E.M Tuzcu, S E Nissen
Journal: Circulation (2008)
Diurnal Changes in Perceptions of Energy and Mood
Authors: C Wood, M E Magnello
Journal: Journal of the Royal Society of Medicine (1992)

Data from five separate groups of volunteers show that diurnal variation in perceptions of physical and mental energy (‘vigour’) can be simply measured by the use of visual analogue scales. In all five groups, perceptions of vigour were significantly higher in the morning than at other times of day. In three groups where energy levels were measured six times daily the values achieved between 10.00 h and 12.00 h were significantly higher (P&lt; 0.05) than those recorded on rising or retiring. Some evidence was also found for the existence of high energy levels in the evening (particularly among the undergraduates) and for an ‘afternoon dip’ in energy in about 20% of subjects. Higher levels of positive affect were also found in the morning and levels recorded between 10.00 h and 12.00 h were significantly greater than those on rising or retiring, (P&lt; 0.01). However, no similar diurnal variation was observed for negative affect. The difference may be due to the fact that positive affect has a distinct ‘biological’ component contributing to perceptions of energy, whilst negative affbct depends more on features in the individual's environment, which show no consistent diurnal pattern.
Abstract 2840: An Impedance Threshold Device Improves 24 Hour Survival in a Spontaneously Breathing Pediatric Porcine Model of Hemorrhagic Shock
Authors: Anja Metzger, Brad Marino, Tim Matsuura, Carly Alexander, Margot Herman, Scott McKnite, Vijay Srinivasan, Vinay Nadkarni, Keith Lurie
Journal: Circulation (2007)

Introduction: Traumatic injury and hypovolemic shock are leading causes of death in children worldwide. We previously demonstrated that augmentation of negative intrathoracic pressure generated by spontaneously breathing through an impedance threshold device (ITD) improves blood pressure and cardiac output in a pediatric porcine model of hemorrhagic shock. However, the optimal method to restore intravascular volume, prevent secondary organ damage, and prevent progression of reversible shock to irreversible circulatory collapse after severe blood loss in children is not known. Hypothesis: Breathing through an ITD for 90 min prior to fluid resuscitation will safely increase short and long-term survival rates. Methods: Seventeen spontaneously breathing female piglets (12.4 ± 0.1 kg), anesthetized with propofol, were subjected to a 40% bleed and randomized in a prospective, blinded manner to either a sham or active ITD (cracking pressure of -7 cmH2O, Advanced Circulatory Systems, Minneapolis, MN) for 90 min. Shed blood was then reinfused. Work of breathing (WOB), 90 minute and 24 hour survival were evaluated. Survival analysis was performed using the Wilcoxon Rank Sum Test, Fischer’s Exact Test, and Kaplan-Meier Methods. Results: Baseline and post-bleed heart rate, blood pressure, lactate levels, and arterial blood gas parameters were similar between groups. Piglets treated with the active ITD had significantly improved survival compared to the piglets treated with the sham ITD at 90 min (8/9 vs. 3/8, p=0.04). At 24 hours, Kaplan-Meier curve analysis revealed improved survival with the active ITD (6/9 vs. 2/8, p=0.04). The 3 animals in the active ITD group that did not survive died at a median time of 280 minutes (82–580), and the 6 animals in the sham group that did not survive, died at a median time of 69 minutes (24 –101) after bleed (p=0.11). The WOB at 45 min (J/L) was 73% higher in the active ITD group than the sham group (1.18 ± 0.07 vs. 0.68 ± 0.07, p= 0.001). The piglets safely tolerated the active ITD therapy. Conclusion: In this spontaneously breathing, anesthetized piglet model of hemorrhagic shock, treatment with an active ITD for 90 min without supplemental fluid resuscitation significantly improved short and long-term survival rates.
Abstract 17706: A Common Variant in the PTPN11 Gene Contributes to the Risk of Tetralogy of Fallot
Authors: Bernard Keavney, Chrysovalanto Mamasoula, Darroch Hall, Ana Topf, Helen Griffin, Thahira Rahman, Heather Cordell, Judith Goodship
Journal: Circulation (2011)

Background: Tetralogy of Fallot (TOF) is the commonest form of cyanotic congenital heart disease. In 80% of cases, TOF behaves as a complex disease exhibiting significant heritability. We investigated whether common genetic variation in 23 candidate genes, selected either because of their expression in the second heart field or their involvement in syndromic congenital heart disease, predisposes to risk of “sporadic”, non-syndromic TOF. Methods and Results: 207 haplotype-tagging single nucleotide polymorphisms (SNPs) in the candidate genes with minor allele frequencies ≥0.05 were genotyped in a test cohort comprising 362 non-syndromic British Caucasian cases of TOF, and 900 controls (717 unaffected parents of cases, and 183 unrelated healthy people). Six SNPs with suggestive evidence of association (p&lt;0.01) were taken forward for genotyping in a replication cohort comprising 392 cases of TOF, 218 unaffected parents of cases, and 1319 controls. Significant association at p&lt;0.01 was confirmed for one SNP, rs11066320 in the PTPN11 gene, in the replication cohort. Genotype at rs11066320 was associated with a per-allele odds ratio of 1.35 (95% CI 1.20-1.52; p=2.94x10-6) in the total cohort of TOF cases and controls. Genotype at rs11066320 was responsible for a population attributable risk of TOF of approximately 5%. Association between rs11066320 genotype and TOF remained significant after Bonferroni correction for 207 analyses (corrected p=0.00061) Conclusions: Common variation in the PTPN11 gene contributes to the risk of non-syndromic TOF.
Abstract 54: Does Telephone-Assisted Cardiopulmonary Resuscitation Improve the Outcome of Out-of-Hospital Cardiac Arrest?
Authors: Thomas Loeb, Annna Ozguler, Geraldine Baer, Charles Groizard, Michel Baer
Journal: Circulation (2012)

Objectives: In an effort to increase the proportion of victims who receive bystander cardiopulmonary resuscitation (CPR) in case of out-of-hospital cardiac arrest (OHCA), it is now clearly recommended to propose t-CPR. However, very few studies have demonstrated, in real life conditions, the clinical benefits of such recommendations. The aim of this study was to assess the survival benefit of t-CPR on OHCA, compared with no bystander CPR. Materials and methods: This study was conducted over a seven-month period in 2011. It included all incoming calls to an Emergency Medical Service dispatch center in which t-CPR was instructed to non-priorly trained bystanders. The comparison group was matched on the date of occurrence; we enrolled incoming calls for witnessed OHCA with no bystander-CPR performed before rescuers arrival. Variables were given as means or percentages. Data were compared using Chi-square tests and Student’s t-test Results: The results appear in Table 1. Conclusion : This study showed that in OHCA, when bystanders initiated t-CPR, despite 1- a younger age population 2- more advanced life support and 3- higher rates of ROSC prior to hospital arrival, there was no improvement in survival rate after ICU discharge. As t-CPR does not seem sufficient to improve outcome, training citizens in CPR remains a top priority.
Human African trypanosomiasis in a Belgian traveller returning from the Masai Mara area, Kenya, February 2012
Authors: J Clerinx, E Vlieghe, V Asselman, S Van de Casteele, M B Maes, V Lejon
Journal: Eurosurveillance (2012)

A Belgian traveller was diagnosed with human African trypanosomiasis (HAT) due to Trypanosoma brucei rhodesiense nine days after visiting the Masai Mara area in Kenya. He presented with an inoculation chancre and was treated with suramin within four days of fever onset. Two weeks earlier, HAT was also reported in a German traveller who had visited the Masai Mara area. Because no cases have occurred in the area for over 12 years, this may indicate a focal cluster of HAT.
Abstract 2542: Effect of Lipid Lowering Therapy on Progression of Coronary Fibroatheroma Evaluated by Virtual Histology Intravascular Ultrasound
Authors: Kenya Nasu, Etsuo Tsuchikane, Osamu Katoh, Masashi Kimura, Mariko Ehara, Yoshihisa Kinoshita, Hitoshi Matsuo, Yasushi Asakura, Tetsuo Matsubara, Mitsuyasu Terashima, Takahiko Suzuki
Journal: Circulation (2008)

Background: The aim of this study is to asses the effect of 12 month treatment with Fluvastatin (60 mg daily) on progression of fibroatheroma in a non-culprit vessel. Methods: This prospective study was performed at 2 centers and designed to enroll 80 patients. Patients were randomized to receive lipid-lowering therapy by Fluvastatin or a lipid lowering diet. All patients with high low-density lipoprotein-cholesterol level (&gt; 140 mg/dl) were included in the Fluvastatin group. Non-culprit vessels were imaged with a 20 MHz phased-array intravascular ultrasound (IVUS) catheter with motorized pull-back system at 0.5 mm/s. Virtual Histology-derived fibroatheroma (VHD-FA) was defined as 1) percent necrotic core area &gt;20%; 2) percent plaque plus media cross-sectional area &gt;40%. For each image, the area of vessel and each plaque component were calculated. At 12-month followup repeat IVUS measurements were performed. Results: A total of 44 VHD-FAs in the Fluvastatin group and 47 VHD-FAs in the control group were detected at baseline. In the Fluvastatin group, fibrous and fibro-fatty areas are significantly decreased, necrotic core did not increase, and there was a significant reduction in atherogenic lipoproteins. In the control group, fibrous, necrotic core and dense calcium areas significantly increased (table ). Conclusion: Lipid-lowering therapy with Fluvastatin prevented progression of coronary fibroatheroma. Laboratory data and IVUS measurements
Factors Influencing Appropriate Firing of the Implanted Defibrillator for Ventricular Tachycardia/Fibrillation
Authors: Jagmeet P. Singh, W. Jackson Hall, Scott McNitt, Hongyue Wang, James P. Daubert, Wojciech Zareba, Jeremy N. Ruskin, Arthur J. Moss
Journal: Journal of the American College of Cardiology (2005)
Abstract 18013: Impact of Chronic Kidney Disease on Risk of Incident Atrial Fibrillation and Subsequent Survival in Medicare Patients
Authors: Shuling Li, Charles A Herzog
Journal: Circulation (2010)

Introduction: Atrial Fibrillation (AF) is common in elderly pts and its prevalence increased in pts with chronic kidney disease (CKD). Few studies have examined CKD as a risk factor for incident AF...
Race Culture; or, Race Suicide? A Plea for the Unborn.
Authors: Robert Reid Rentoul
Journal: JAMA (1907)

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Cost analysis of a randomized trial of early palliative care (PC) in patients with metastatic non-small cell lung cancer (NSCLC).
Authors: Joseph A. Greer, Angela Tramontano, Pamela M McMahon, Areej El-Jawahri, Ravi Bharat Parikh, Emily R. Gallagher, William F. Pirl, Vicki A. Jackson, Alona Muzikansky, Jennifer S. Temel
Journal: Journal of Clinical Oncology (2014)

4 Background: Several randomized, controlled trials have shown that early, integrated palliative and oncology care improves quality of life, mood, and symptom burden in patients with advanced cancers. However, the degree to which early involvement of specialty PC in the ambulatory care setting impacts the cost of care remains unknown. We investigated the health care costs for patients with metastatic NSCLC enrolled in a clinical trial of early PC. Methods: For this secondary analysis, we examined data from a randomized trial of 151 patients with newly-diagnosed metastatic NSCLC from 06/2006 to 07/2009. Patients received either early PC integrated with standard care or standard care (SC) alone. We abstracted costs for emergency and inpatient care, outpatient visits, intravenous chemotherapy, and physician services from the hospital’s accounting system. Oral chemotherapy costs were estimated based on actual drug charges for patients. To estimate hospice costs, we used Medicare reimbursement rates. For each participant, we calculated the average total cost of care per day for the entire study period as well as the total cost of care for the final 30 days prior to death. Costs differences between groups were examined with the Wilcoxon Rank-Sum Test. Results: We analyzed health care costs of the 138 patients who died by 07/15/2013 (early PC N=68; SC N=70). The mean number of days on study was longer for patients assigned to early PC (M=397, SD=360) versus SC (M=299, SD=266). Over the study period, early PC was associated with a lower average total cost per day of $117 (SD=$436) compared to SC (p=.09). In the final 30 days of life, patients in the early PC group incurred higher total costs for hospice care (Mean difference=$1,053, SD=$3,162, p=.11), while expenses for chemotherapy were less (Mean difference=$757, SD=$2,143, p=.06). No cost differences between groups met the threshold for statistical significance. Conclusions: Although this secondary analysis was inconclusive due to the lack of statistical power to examine differences in cost outcomes, the delivery of early PC for patients with metastatic NSCLC does not appear to increase health care expenses over the course of disease or at the end of life. Clinical trial information: NCT01038271.
Eplerenone Survival Benefits in Heart Failure Patients Post-Myocardial Infarction Are Independent From its Diuretic and Potassium-Sparing Effects
Authors: Patrick Rossignol, Joël Ménard, Renaud Fay, Finn Gustafsson, Bertram Pitt, Faiez Zannad
Journal: Journal of the American College of Cardiology (2011)