tbuckley/cabot-search huggingface dataset:
Protein arginine N-methyltransferase 4 (PRMT4) contributes to lymphopenia in experimental sepsis
Authors: Yandong Lai, Xiuying Li, Tiao Li, Xiaoyun Li, Toru Nyunoya, Kong Chen, Georgios Kitsios, Mehdi Nouraie, Yingze Zhang, Bryan J McVerry, Janet S Lee, Rama K Mallmapalli, Chunbin Zou
Journal: Thorax (2022)

Background One hallmark of sepsis is the reduced number of lymphocytes, termed lymphopenia, that occurs from decreased lymphocyte proliferation or increased cell death contributing to immune suppression. Histone modification enzymes regulate immunity by their epigenetic and non-epigenetic functions; however, the role of these enzymes in lymphopenia remains elusive. Methods We used molecular biological approaches to investigate the high expression and function of a chromatin modulator protein arginine N-methyltransferase 4 (PRMT4)/coactivator-associated arginine methyltransferase 1 in human samples from septic patients and cellular and animal septic models. Results We identified that PRMT4 is elevated systemically in septic patients and experimental sepsis. Gram-negative bacteria and their derived endotoxin lipopolysaccharide (LPS) increased PRMT4 in B and T lymphocytes and THP-1 monocytes. Single-cell RNA sequencing results indicate an increase of PRMT4 gene expression in activated T lymphocytes. Augmented PRMT4 is crucial for inducing lymphocyte apoptosis but not monocyte THP-1 cells. Ectopic expression of PRMT4 protein caused substantial lymphocyte death via caspase 3-mediated cell death signalling, and knockout of PRMT4 abolished LPS-mediated lymphocyte death. PRMT4 inhibition with a small molecule compound attenuated lymphocyte death in complementary models of sepsis. Conclusions These findings demonstrate a previously uncharacterised role of a key chromatin modulator in lymphocyte survival that may shed light on devising therapeutic modalities to lessen the severity of septic immunosuppression.
Prevalence of Hidradenitis Suppurativa in Berekum, Ghana
Authors: Paa Gyasi Hagan, Dorra Bouazzi, Gideon Nyarko, Elsie Serwaa Dartey, Kojo Botsie Nunoo-Ghartey, David Nkum, Prince Ansu, Joel Yiadom Boakye, Rune Kjærsgaard Andersen, Jurr Boer, Gregor B.E. Jemec
Journal: British Journal of Dermatology (2022)
Injectable adhesive hemostatic gel with tumor acidity neutralizer and neutrophil extracellular traps lyase for enhancing adoptive NK cell therapy prevents post-resection recurrence of hepatocellular carcinoma
Authors: Yusheng Cheng, Yihang Gong, Xiuxing Chen, Qi Zhang, Xijian Zhang, Yizhan He, Lijie Pan, Beibei Ni, Fan Yang, Yan Xu, Lei Zhou, Yang Yang, Wenjie Chen
Journal: Biomaterials (2022)
MULTIDIMENSIONAL POVERTY AS A SOCIAL DETERMINANT OF HEALTH FOR ATHEROSCLEROTIC CARDIOVASCULAR DISEASE IN THE UNITED STATES
Authors: Sara Butt, Mauricio Tano, Javier Valero-Elizondo, Zulqarnain Javed, Kobina Hagan, Isaac Acquah, Elias Mossialos, Zlatko Nikoloski, Miguel Cainzos Achirica, Khurram Nasir
Journal: Journal of the American College of Cardiology (2022)
Nationwide indoor smoking ban and impact on smoking behaviour and lung function: a two-population natural experiment
Authors: Alexandra Strassmann, Yunus Çolak, Miquel Serra-Burriel, Børge G Nordestgaard, Alexander Turk, Shoaib Afzal, Milo A Puhan
Journal: Thorax (2022)

Introduction Many countries have implemented indoor smoking bans over the past two decades. Although smoking bans have been shown to reduce cardiovascular outcomes, little is known about their impact on respiratory health. This study investigated the impact of a nationwide indoor smoking ban on smoking behaviour and lung function. Methods We used repeated cross-sectional data from two large cohorts of the general population comprising 31 807 Swiss and 62 093 Danish adults. We compared associations between smoking ban and smoking prevalence and prebronchodilator lung function trends in Denmark (indoor smoking ban introduced in 2007) and Switzerland (indoor smoking ban introduced in 2010) from 2005 to 2010 using a quasi-experimental study design. We performed difference-in-difference analyses with linear regression models adjusted for age, sex, weight and height. Results Denmark had a stronger decrease in active smokers compared with Switzerland. Also, forced expiratory volume in the first second was higher in Danish adults than in Swiss adults: 26 mL (95% CI 2.4 to 49) 1 year, 88 mL (65 to 112) 2 years, and 74 mL (51 to 98) 3 years after smoking ban implementation. Correspondingly, forced vital capacity was higher in Danish adults compared with Swiss adults (80 mL (50 to 109) after 1 year and 126 mL (97 to 155) after two and 3 years). Improvements were observed in both never-smokers and ever-smokers, most pronounced in ever-smokers. Conclusions Nationwide indoor smoking ban is associated with less smoking and improved lung function in the general population. Implementing an indoor smoking ban can improve lung function by influencing smoking behaviour and reducing secondhand smoke.
Abstract P1-08-11: Clinical relevance of host immunity in patients with breast cancer who received neoadjuvant chemotherapy
Authors: Soong June Bae, Jung Hwan Ji, Chihhao Chu, Yoon Jin Cha, Jee Hung Kim, Sung Gwe Ahn, Joon Jeong
Journal: Cancer Research (2022)

Abstract Background Host immunity plays a key role in breast cancer progression. It seems that the neutrophil-to-lymphocyte ratio (NLR) may reflect the systemic host immunity, and tumor-infiltrating lymphocytes (TILs) may reflect the localized host immunity. This study aimed to analyze the clinical relevance of host immunity combining NLR and TILs to treatment efficacy and survival.Methods We assessed the pre-treatment NLR and TILs from core-biopsy samples in 461 patients with breast cancer who received neoadjuvant chemotherapy. The patients were assigned into two groups: the intact host immunity group (NLR &amp;lt; 2.75 and TILs ≥ 30%) and the defective host immunity group (the remainder; NLR &amp;gt; 2.75 or TILs &amp;lt; 30%). We investigated the pathologic complete response (pCR) and recurrence-free survival (RFS) according to host immunity.ResultsOf the total, 141 (30.6%) patients belong to the intact host immunity group, and 320 (69.4%) patients belong to the defective host immunity group. A pCR was achieved in 76 (53.9%) of 141 patients in the intact host immunity group, whereas a pCR was achieved in 96 (30.0%) of 320 patients in the defective host immunity group (P&amp;lt;0.001). The intact host immunity was an independent predictor for high pCR (adjusted odds ratio [OR], 2.65; 95% confidence interval [CI], 1.74-4.03). Furthermore, the Cox proportional hazards regression models revealed that the intact host immunity obtained an independent prognostic value for favorable RFS after adjusted for clinicopathologic factors including pCR (adjusted hazard ratio [HR], 0.33; 95% CI, 0.11-0.98; P=0.045).ConclusionHost immunity based on the combined analysis of NLR and TILs provides information regarding the treatment response and prognosis in patients with breast cancer receiving neoadjuvant chemotherapy. There is a need for a new therapeutic strategy to activate the immune system in patients with defective host immunity. 2 Citation Format: Soong June Bae, Jung Hwan Ji, Chihhao Chu, Yoon Jin Cha, Jee Hung Kim, Sung Gwe Ahn, Joon Jeong. Clinical relevance of host immunity in patients with breast cancer who received neoadjuvant chemotherapy [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr P1-08-11.
Notice of Retraction and Replacement. Anderson et al. Association of Marijuana Legalization With Marijuana Use Among US High School Students, 1993-2019. <i>JAMA Netw Open</i>. 2021;4(9):e2124638.
Authors: D. Mark Anderson, Joseph J. Sabia
Journal: JAMA Network Open (2022)

These concerns were brought to our attention by readers and subsequently published as Comments with the article. 2,3In this study, we used data from the Youth Risk Behavior Survey (YRBS) for the period 1993 to 2019 to provide updated estimates of the association of legalization of recreational marijuana and legalization of medical marijuana with adolescent marijuana use.For our analyses, we used unweighted and pooled national and state YRBS data.While this unweighted and pooled approach maximized the number of state policy changes used to identify the effect of marijuana legalization on youth marijuana use for the average student in the pooled sample, published Comments advised that the approach was inappropriate for the following reasons: (1) "Pooling national and state YRBS data is inappropriate because underlying person-level weights are different, and there is some overlap between national and state YRBS data; thus, a student could be represented more than once.The YRBS codebook explicitly warns against combining these data."And "YRBS data are designed to be weighted to capture representative populations" 3 In addition, unweighted and pooled analyses may produce results that are not generalizable.At the request of
Resection of glioma—Feeding the beast?
Authors: Jens Blobner, Joerg-Christian Tonn
Journal: Neuro-Oncology (2022)

It is well established that glioma cell infiltration extends far beyond the radiological limits of the tumor. Even more, glioma cells and brain parenchyma develop a functional multicellular network structure by using tumor microtubes formed by membrane protrusions as routes for brain invasion, proliferation and to interconnect over long distances. Therefore, these tumors should be considered a systemic brain disease rather than a delineated tumor.1 Given this diffuse invasion pattern, an oncological “radical” resection is virtually impossible in the majority of cases. Yet, surgical excision is one central pillar in the treatment strategy.2 However, there is definitional fuzziness regarding categories for extent of resection. Prior studies quantify the percentage of tumor volume reduction while more recent studies suggest the absolute residual tumor volume to be more relevant in terms of prognosis.3 Although a series of studies have reported a survival benefit when parts of the surrounding T2/FLAIR-hyperintense region have been resected, further evaluation of the clinical and biological impacts of supramarginal resection in both low- and high-grade gliomas are warranted. Especially in malignant gliomas, these studies will also have to take into account whether the mere “survival benefit” outweighs potential impairment of morbidity and postsurgical quality of life.
B-cell responses to vaccination with BNT162b2 and mRNA-1273 6 months after second dose
Authors: Robert Markewitz, Daniela Pauli, Justina Dargvainiene, Katja Steinhagen, Sarah Engel, Victor Herbst, Dorinja Zapf, Christina Krüger, Shahpour Sharifzadeh, Benjamin Schomburg, Frank Leypoldt, Jan Rupp, Siegfried Görg, Ralf Junker, Klaus-Peter Wandinger
Journal: Clinical Microbiology and Infection (2022)
Comparative analysis of surface sanitization protocols on the bacterial community structures in the hospital environment
Authors: Tilman E. Klassert, Cristina Zubiria-Barrera, Robert Neubert, Magdalena Stock, Antony Schneegans, Mercedes López, Dominik Driesch, Gregor Zakonsky, Petra Gastmeier, Hortense Slevogt, Rasmus Leistner
Journal: Clinical Microbiology and Infection (2022)

In hospital hygiene, it remains unclear to what extent surface contamination might represent a potential reservoir for nosocomial pathogens. This study investigates the effects of different sanitization strategies on the microbial structures and the ecological balance of the environmental microbiome in the clinical setting.Three cleaning regimes (disinfectants, detergents, and probiotics) were applied subsequently in nine independent patient rooms at a neurological ward (Charité, Berlin). Weekly sampling procedures included three different environmental sites: floor, door handle, and sink. Characterization of the environmental microbiota and detection of antibiotic resistance genes (ARGs) were performed by 16S rRNA sequencing and multiplex Taq-Man qPCR assays, respectively.Our results showed a displacement of the intrinsic environmental microbiota after probiotic sanitization, which reached statistical significance in the sink samples (median 16S-rRNA copies = 138.3; IQR: 24.38-379.5) when compared to traditional disinfection measures (median 16S rRNA copies = 1343; IQR: 330.9-9479; p < 0.05). This effect was concomitant with a significant increase in the alpha-diversity metrics in both the floor (p < 0.001) and the sink samples (p < 0.01) during the probiotic strategy. We did not observe a sanitization-dependent change in relative pathogen abundance at any tested site, but there was a significant reduction in the total ARG counts in the sink samples during probiotic cleaning (mean ARGs/sample: 0.095 ± 0.067) when compared to the disinfection strategy (mean ARGs/sample: 0.386 ± 0.116; p < 0.01).The data presented in this study suggest that probiotic sanitization is an interesting strategy in hospital hygiene management to be further analyzed and validated in randomized clinical studies.
INCIDENCE AND OUTCOMES OF IN-HOSPITAL CARDIAC ARREST DURING THE CORONAVIRUS DISEASE 2019 PANDEMIC AND THE PRECEDING ERA: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Sanjana Nagraj, Sumit Sohal, Dimitrios Varrias, Andreas Tzoumas, Spyros Peppas, Marko Novakovic, Sheetal V. Mathai, Damianos Kokkinidis, Rosy Thachil, Robert T. Faillace
Journal: Journal of the American College of Cardiology (2022)
Correction: Clinical and technical outcomes of patients undergoing endoscopic ultrasound-guided gastroenterostomy using 20-mm vs. 15-mm lumen-apposing metal stents
Authors: Michael Bejjani, Bachir Ghandour, Jose Carlos Subtil, Belén Martínez-Moreno, Reem Z. Sharaiha, Rabindra R. Watson, Thomas E. Kowalski, Petros C. Benias, Matthew T. Huggett, Tobias Weber, Lionel S. D’Souza, Andrea Anderloni, Michael Lajin, Harshit S. Khara, Khanh Do-Cong Pham, Douglas Pleskow, Carlo Fabbri, Jose M. Nieto, Nikhil A. Kumta, Rishi Pawa, Manol Jovani, Mouen A. Khashab
Journal: Endoscopy (2022)

Correction to: Clinical and technical outcomes of patients undergoing endoscopic ultrasound-guided gastroenterostomy using 20-mm vs. 15-mm lumen-apposing metal stentsEndoscopy 2022; 54(07): 680-687DOI: 10.1055/a-1654-6914
Structure remodeling of soy protein-derived amyloid fibrils mediated by epigallocatechin-3-gallate
Authors: Zejian Xu, Guancheng Shan, Nairong Hao, Lianwei Li, Tian Lan, Yabo Dong, Jiayu Wen, Ran Tian, Yan Zhang, Lianzhou Jiang, Xiaonan Sui
Journal: Biomaterials (2022)
TOTAL CARDIOVASCULAR AND LIMB EVENTS WITH TICAGRELOR AND CLOPIDOGREL IN PATIENTS WITH SYMPTOMATIC PAD IN THE EUCLID TRIAL
Authors: Michael Szarek, Connie Ng Hess, Manesh R. Patel, William Schuyler Jones, Jeffrey S. Berger, Iris Baumgartner, Brian Katona, Kenneth W. Mahaffey, Lars Norgren, Juuso Blomster, Frank Rockhold, Judith A. Hsia, Francis Gary Fowkes, Marc P. Bonaca
Journal: Journal of the American College of Cardiology (2022)
Re: Leclercq et al.: Anti-tumor necrosis factorα versus tocilizumab in the treatment of refractory uveitic macular edema: a multicenter study from the French Uveitis Network. (Ophthalmology. 2021 Nov 16;S0161-6420(21)00900-3. doi: 10.1016/j.ophtha.2021.11.013. Online ahead of print. [Epub ahead of print]
Authors: François Pépin, Rachael L. Niederer
Journal: Ophthalmology (2022)
Corrigendum to ‘Risk stratification and management of women with cardiomyopathy/heart failure planning pregnancy or presenting during/after pregnancy: a position statement from the Heart Failure Association of the European Society of Cardiology Study Group on Peripartum Cardiomyopathy’ [<i>Eur J Heart Fail</i> 2021;23:527–540]
Journal: European Journal of Heart Failure (2022)
INPATIENT HOSPITAL MORTALITY IN TAKOTSUBO CARDIOMYOPATHY (TCM): INSIGHTS FROM THE NATIONAL INPATIENT SAMPLE
Authors: Mohsin Mughal, Weiyi Xia, Hasan Mirza, Harjot S. Jagdey, Ali Ghani, Muhammad Khakwani, Hafsa Akbar, Hamza Hafeez, Muhammad Zahid A. Raza, Howard L. Levitt, Fahad Waqar, Najam Wasty, Mohammed Haris Usman, Mahboob Alam, Sergio Waxman
Journal: Journal of the American College of Cardiology (2022)
EVALUATION OF EARLY ENDOTHELIALIZATION 1 MONTH AFTER STENTING USING OPTICAL COHERENCE TOMOGRAPHY AND 3-YEAR CLINICAL OUTCOMES OF DUAL-THERAPY ENDOTHELIAL PROGENITOR CELL CAPTURING SIROLIMUS-ELUTING COMBO STENT: THE OCT-VENTURE STUDY
Authors: Jiachen BI, William Kongto Hau, Bryan P. Yan
Journal: Journal of the American College of Cardiology (2022)
12P Circulating tumor DNA as early marker of response to treatment in stage IV gastrointestinal cancer
Authors: P. Kirchweger, A. Kupferthaler, J. Burghofer, G. Webersinke, E. Jukic, S. Schwendinger, R. Függer, M. Biebl, H. Wundsam, H. Rumpold
Journal: Annals of Oncology (2022)
DIGITAL HEALTH INTERVENTIONS AFTER HOSPITALIZATION FOR DECOMPENSATED HEART FAILURE: A SYSTEMATIC REVIEW AND META-ANALYSIS OF RANDOMIZED CONTROLLED TRIALS
Authors: Yazan Assaf, Ayman Elbadawi, Bryan Tan, Michael Megaly, Mohamed Shokr, Mohamed Hamed, Faisal Rahman, Carl J. Pepine, Ahmed Soliman
Journal: Journal of the American College of Cardiology (2022)
RISK OF VENTRICULAR TACHYCARDIA AND SUDDEN CARDIAC ARREST ASSOCIATED WITH QT INTERVAL-PROLONGING DRUGS IN PATIENTS WITH HEART FAILURE WITH PRESERVED EJECTION FRACTION
Authors: Chien-Yu Huang, Brian Overholser, Kevin Sowinski, Heather A. Jaynes, Richard J. Kovacs, James Edward Tisdale
Journal: Journal of the American College of Cardiology (2022)
Batf-mediated epigenetic control of effector CD8 <sup>+</sup> T cell differentiation
Authors: Hsiao-Wei Tsao, James Kaminski, Makoto Kurachi, R. Anthony Barnitz, Michael A. DiIorio, Martin W. LaFleur, Wataru Ise, Tomohiro Kurosaki, E. John Wherry, W. Nicholas Haining, Nir Yosef
Journal: Science Immunology (2022)

The response of naive CD8 + T cells to their cognate antigen involves rapid and broad changes to gene expression that are coupled with extensive chromatin remodeling, but the mechanisms governing these changes are not fully understood. Here, we investigated how these changes depend on the basic leucine zipper ATF-like transcription factor Batf, which is essential for the early phases of the process. Through genome scale profiling, we characterized the role of Batf in chromatin organization at several levels, including the accessibility of key regulatory regions, the expression of their nearby genes, and the interactions that these regions form with each other and with key transcription factors. We identified a core network of transcription factors that cooperated with Batf, including Irf4, Runx3, and T-bet, as indicated by their colocalization with Batf and their binding in regions whose accessibility, interactions, and expression of nearby genes depend on Batf. We demonstrated the synergistic activity of this network by overexpressing the different combinations of these genes in fibroblasts. Batf and Irf4, but not Batf alone, were sufficient to increase accessibility and transcription of key loci, normally associated with T cell function. Addition of Runx3 and T-bet further contributed to fine-tuning of these changes and was essential for establishing chromatin loops characteristic of T cells. These data provide a resource for studying the epigenomic and transcriptomic landscape of effector differentiation of cytotoxic T cells and for investigating the interdependency between transcription factors and its effects on the epigenome and transcriptome of primary cells.
“RECUMBENT BIKE TO THE RESCUE”: UTILITY OF INVASIVE HEMODYNAMIC ASSESSMENT WITH EXERCISE ON SUPINE BICYCLE IN A PATIENT WITH HYPERTROPHIC CARDIOMYOPATHY (HCM) WITHOUT LEFT VENTRICULAR OUTFLOW TRACT OBSTRUCTION (LVOTO)
Authors: Manojna Nimmagadda, Abdullah Sarkar, Diana C. Miranda Ruiz, Antonio Lewis, Jose Sleiman, Eduardo Javier Perez, Alejandro Sanchez Nadales, Dipan Uppal, David Snipelisky, Craig R. Asher
Journal: Journal of the American College of Cardiology (2022)
Focusing on Accessibility of Evidence-Based Treatments for Obsessive-Compulsive Disorder
Authors: Emily G. Lattie, Caitlin A. Stamatis
Journal: JAMA Network Open (2022)

Lina Lundström, MSc; Oskar Flygare, MSc; Erik Andersson, PhD; Jesper Enander, PhD; Matteo Bottai, PhD; Volen Z. Ivanov, PhD; Julia Boberg, MSc; Diana Pascal, MD, PhD; David Mataix-Cols, PhD; Christian Rück, MD, PhD
Disruption of mitochondrial complex III in cap mesenchyme but not in ureteric progenitors results in defective nephrogenesis associated with amino acid deficiency
Authors: Nan Guan, Hanako Kobayashi, Ken Ishii, Olena Davidoff, Feng Sha, Talat A. Ikizler, Chuan-Ming Hao, Navdeep S. Chandel, Volker H. Haase
Journal: Kidney International (2022)

Oxidative metabolism in mitochondria regulates cellular differentiation and gene expression through intermediary metabolites and reactive oxygen species. Its role in kidney development and pathogenesis is not completely understood. Here we inactivated ubiquinone-binding protein QPC, a subunit of mitochondrial complex III, in two types of kidney progenitor cells to investigate the role of mitochondrial electron transport in kidney homeostasis. Inactivation of QPC in sine oculis-related homeobox 2 (SIX2)-expressing cap mesenchyme progenitors, which give rise to podocytes and all nephron segments except collecting ducts, resulted in perinatal death from severe kidney dysplasia. This was characterized by decreased proliferation of SIX2 progenitors and their failure to differentiate into kidney epithelium. QPC inactivation in cap mesenchyme progenitors induced activating transcription factor 4-mediated nutritional stress responses and was associated with a reduction in kidney tricarboxylic acid cycle metabolites and amino acid levels, which negatively impacted purine and pyrimidine synthesis. In contrast, QPC inactivation in ureteric tree epithelial cells, which give rise to the kidney collecting system, did not inhibit ureteric differentiation, and resulted in the development of functional kidneys that were smaller in size. Thus, our data demonstrate that mitochondrial oxidative metabolism is critical for the formation of cap mesenchyme-derived nephron segments but dispensable for formation of the kidney collecting system. Hence, our studies reveal compartment-specific needs for metabolic reprogramming during kidney development.
Food Sensitization and DNA methylation at age one in the CHILD Cohort
Authors: Kurt Kolsun, Samantha Lee, Julia MacIsaac, Theo Moraes, Padmaja Subbarao, Meghan Azad, Piush Mandhane, Stuart Turvey, Michael Kobor, Meaghan Jones, Elinor Simons
Journal: Journal of Allergy and Clinical Immunology (2022)
Response to Comment on Dawed et al. Genome-Wide Meta-analysis Identifies Genetic Variants Associated With Glycemic Response to Sulfonylureas. Diabetes Care 2021;44:2673–2682
Authors: Adem Y. Dawed, Sook Wah Yee, Kaixin Zhou, Nienke van Leeuwen, Yanfei Zhang, Moneeza K. Siddiqui, Amy Etheridge, Federico Innocenti, Fei Xu, Josephine H. Li, Joline W. Beulens, Amber A. van der Heijden, Roderick C. Slieker, Yu-Chuan Chang, Josep M. Mercader, Varinderpal Kaur, John S. Witte, Ming Ta Michael Lee, Yoichiro Kamatani, Yukihide Momozawa, Michiaki Kubo, Colin N.A. Palmer, Jose C. Florez, Monique M. Hedderson, Leen M. 't Hart, Kathleen M. Giacomini, Ewan R. Pearson
Journal: Diabetes Care (2022)