OP0307 GUT MICROBIOTA AND ITS RELEVANCE TO PERIPHERAL LYMPHOCYTE SUBPOPULATION IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS
Authors: T. Cheng, X. Wang, S. X. Zhang, J. Yang, C. Zhao, Y. Wang, J. An, J. Chen
Journal: Annals of the Rheumatic Diseases (2021)

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease with disturbance of lymphocyte subpopulations 1 . Growing experimental and clinical evidence suggests that chronic inflammatory response induced by gut microbiome critically contribute to the development of SLE 2 3 . Objectives: To investigate the characteristics of gut microbiome and the associations between flora and peripheral lymphocyte subpopulations in SLE patients. Methods: A total of 19 SLE patients who fulfilled the 2019 American college of Rheumatology (ACR) and European League Against Rheumatism (EULAR) classification criteria and 16 age- and sex- matched healthy controls (HC) were enrolled in this study. The peripheral T lymphocyte subsets of these participants were assessed by flow cytometry and the gut microbiota were investigated via 16s rRNA. Indicators of disease activity such as erythrocyte sedimentation rate (ESR), complement C3 and C4 were recorded at the same time. Mann-Whitney U test was applied to compare T lymphocyte subsets between SLE patients and HC. Spearman analysis was used for calculating correlation between T subsets and highly expressed differential flora as well as their correlation with disease activity indicators. All P -values reported herein were two-tailed and P -value&lt;0.05 was taken as statistically significant. Results: SLE patients had higher proportions of Th17 cells ( P =0.020) and γδT cells ( P =0.018) but lower levels of Treg cells ( P =0.001), Tfh cells ( P =0.018) and Naïve CD4 + T cells ( P =0.004) (Figure 1a-e). The diversity and relative abundance of intestinal flora in patients with SLE were significantly different from those in HCs. Detailly, the α-diversity was decreased in SLE ( P &lt;0.05) (Figure 2a-c). Compared with HC, 11 species of flora were discovered to be distinctly different( P &lt;0.05) (Figure 2d-e). Moreover, there was a significant positive correlation between Treg levels and Ruminococcus2 ( P =0.042), Th17 and Megamonas ( P =0.009), γδT and Streptococcus ( P =0.004) as well as Megamonas ( P =0.003), Tfh and Bacteroides ( P =0.040). Whereas Th1 levels and Bifidobacterium were negatively correlated in these participants ( P =0.005). As for clinical disease measures, there were negative correlations not only between ESR and Treg cells ( P =0.031) but also C4 and the amount of Unclassified Ruminococcaceae ( P =0.032). Conclusion: Abnormality of T cell subsets, especially the level of Naïve CD4 + T, γδT, Tfh, Treg, and Th17 cells contributes to the occurrence and progression of SLE, which may be related to the disturbance of gut microbiota. Therefore it is necessary to attach importance to the alteration of gut microbiota to prevent the outbreak of inflammation and maybe they can be identified as biomarkers predicting disease activity. References: [1]Katsuyama T, Tsokos GC, Moulton VR. Aberrant T Cell Signaling and Subsets in Systemic Lupus Erythematosus. Front Immunol 2018;9:1088. doi: 10.3389/fimmu.2018.01088 [published Online First: 2018/06/06] [2]López P, de Paz B, Rodríguez-Carrio J, et al. Th17 responses and natural IgM antibodies are related to gut microbiota composition in systemic lupus erythematosus patients. Sci Rep 2016;6:24072. doi: 10.1038/srep24072 [published Online First: 2016/04/06] [3]Esmaeili SA, Mahmoudi M, Momtazi AA, et al. Tolerogenic probiotics: potential immunoregulators in Systemic Lupus Erythematosus. J Cell Physiol 2017;232(8):1994-2007. doi: 10.1002/jcp.25748 [published Online First: 2016/12/21] Acknowledgements: This project was supported by National Science Foundation of China (82001740), Open Fund from the Key Laboratory of Cellular Physiology (Shanxi Medical University) (KLCP2019) and Innovation Plan for Postgraduate Education in Shanxi Province (2020BY078). Disclosure of Interests: None declared.