Ruxolitinib in adult patients with secondary hemophagocytic lymphohistiocytosis
Authors: Philip S. Boonstra, Asra Ahmed, Samuel A. Merrill, Ryan A. Wilcox
Journal: American Journal of Hematology (2021)

Hemophagocytic lymphohistiocytosis (HLH) is a devastating hyperinflammatory disorder in adults with protean clinical manifestations that is frequently caused by underlying autoimmune, infectious, or malignant disorders. The hyperinflammatory response ("cytokine storm"), including interferon production, instigated by these secondary disorders is the final common pathway in HLH and a therapeutic vulnerability. Therefore, antagonism of specific cytokines (eg, IL-1, IL-6, IFN-γ) is rational and well supported by pre-clinical and clinical studies. For example, a response to the anti-IFN-γ antibody emapalumab was observed in 63% of previously treated pediatric patients with primary HLH.1 Given the pleiotropic, and in some cases partially redundant, effects of most cytokines implicated in HLH pathogenesis, inhibition of Janus family kinases (JAK), required for cytokine receptor-mediated signaling, is an alternative approach, and is well supported in pre-clinical HLH models.2 Therefore, we launched an investigator-initiated, open-label, single-center study of ruxolitinib in adult patients with secondary HLH. A response, most of which were complete, was observed in all patients. A significant improvement in cytopenias and transfusion independence was achieved within 7 days of treatment initiation. All patients were hospitalized at the time of treatment initiation and were successfully discharged within 2 weeks. No deaths were observed, and the outcomes observed were superior to matched historic controls. Pharmacodynamic studies, including inhibition of STAT1 phosphorylation and reductions in soluble CD163, a surrogate marker for macrophage activation, further supported ruxolitinib's activity in secondary HLH. These results have been previously reported,3 but are potentially more significant in the Covid-19 era, as severe Covid-19 is pathophysiologically related to HLH, and JAK inhibitors (including ruxolitinib) are the subject of ongoing studies in severe Covid-19 [reviewed in4]. Herein we report both updated outcomes, with longer follow up, and summarize our experience with ruxolitinib in HLH patients who were treated "off study". The eligibility criteria, study design and endpoints, and statistical analysis plan have been previously reported.4 Briefly, adult patients (≥18 years) who fulfilled five of eight HLH-2004 diagnostic criteria for HLH were eligible.5 Patients with HLH secondary to an underlying malignancy were excluded, as were patients with known CNS involvement, or patients without adequate renal/hepatic function. This was an open label, single-center, prospective, pilot study, and was approved by the institutional review board and was conducted in accordance with the principles of the Declaration of Helsinki and the International Conference on Harmonization Guidelines for Good Clinical Practice. Patients received oral ruxolitinib 15 mg twice daily on a continuous, 28-day cycle and dose reductions for renal insufficiency and toxicity were permitted. Enrollment occurred between February, 2016 and August, 2019. The primary endpoint was overall survival at 2 months, and additional secondary endpoints included response, duration of response, progression-free and overall survival. Adverse event monitoring was performed by history, physical exam, and laboratory monitoring. All quantifiable signs and laboratory abnormalities included in the diagnostic criteria for HLH were considered evaluable for response. Normalization of all signs and laboratory abnormalities was required for a complete response (CR), and at least 25% improvement in at least two signs/laboratory abnormalities was required for a partial response (PR). At least a 50% worsening in at least two signs/laboratory abnormalities defined progressive disease (PD), and failure to fulfill any of these response criteria was considered stable disease (SD). Duration of response was defined as time between first PR/CR and subsequent SD/PD. Although this pilot study was closed in January, 2020, patients without recurrent HLH were assessed as deemed clinically appropriate by the treating co-investigator after study closure. Patients with a durable PR/CR at the time of the last clinical assessment were censored. Six patients (numbers 1–6) were enrolled in this study, and seven patients (numbers7-13) were treated off study (Table 1). One study patient (#6) enrolled after our initial report,3 and is now included here. Patients treated off study either failed to meet eligibility criteria, were treated after study closure, or were unable to participate due to logistical considerations, and two of these patients (#7, #8) were previously reported. The secondary etiology for HLH in these patients was idiopathic (5/13), autoimmune (4/13), and/or infectious (5/13), as summarized in Table 1. Malignancy-associated HLH was underrepresented, as these patients were not eligible for this study, but a single patient with relapsed classical Hodgkin lymphoma (cHL) and EBV viremia (#11) received treatment off study. All patients satisfied diagnostic criteria for HLH (summarized in Table S1). Ruxolitinib was first-line or second-line therapy for most (9/13) patients, whereas the remaining patients had recurrent or progressive HLH despite multiple prior therapies (range: 2–5 prior lines of therapy). An overall response rate of 77% was observed (Table 1), including five CR and five PR. As previously noted,3 the clinical significance of the distinction between a PR and CR in these patients is doubtful, as patients achieving a PR had persistent, mild elevations in laboratory abnormalities that may have preceded their HLH diagnosis (eg, hypertriglyceridemia, mild elevations in ferritin, and anemia of chronic disease), and thus highlights the need for improved response criteria in this disease. Three patients with severe, life-threatening infections at the time of treatment failed to respond (#10, 12, 13) and died within 5–41 days of treatment initiation. No other deaths have been observed. Two patients developed recurrent HLH after achieving an initial response (#1, 11), both of which are of special interest. One patient (#1) was found to have an underlying mutation (in the STXBP2 gene) associated with primary HLH and underwent allogeneic stem cell transplantation, as previously described.3 The other (#11) had malignancy-associated HLH (relapsed and refractory cHL), thus highlighting the importance of incorporating malignancy-targeted therapies in these patients. Therefore, 62% of patients (8/13) achieved a durable response (duration of response, range: 12.0–41.5+ months). As the optimal duration of therapy is unknown, gradual tapering and discontinuation of ruxolitinib, at the discretion of the investigator, was allowed, although an unspecified gradual taper was recommended, given the potential for cytokine rebound with rapid discontinuation. Therefore, most patients achieving a durable response have successfully discontinued treatment without recurrent HLH (5/8), and three patients remain on treatment. As anticipated, ruxolitinib was well tolerated, with two grade ≥ 3 adverse events (febrile neutropenia and fatigue) that were possibly treatment related (Table 1). One patient discontinued treatment due to grade two extremity pain (#2). The three deaths observed occurred in patients with challenging, difficult to treat infectious complications, thus highlighting the need for early recognition of HLH and prompt treatment initiation.6 With the exception of a single patient with primary HLH, and multiple secondary drivers, the only patient who progressed after achieving an initial, albeit relatively transient, response (#11) had progressive cHL. A response in this patient was only achieved upon control of the underlying cHL, thus highlighting the importance of malignancy-directed therapies in similar patients. In addition, JAK inhibition may be particularly attractive in malignancies, including mature T-cell lymphomas, that are commonly associated with secondary HLH,6 and for which ruxolitinib (or alternative JAK inhibitors) have clinical activity. In our experience, ruxolitinib is active in secondary HLH, well tolerated, non-myelosuppressive, and manageable in the outpatient setting. Therefore, we believe these findings support ongoing efforts investigating JAK inhibitors, not only in HLH, but in other HLH-related cytokine-release syndromes, including severe Covid-19. The work was presented in part at the 60th Annual ASH Meeting & Exposition, December, 2018. This work was supported in part by the National Cancer Institute (K08CA172215 and P30CA046592), the University of Michigan Rogel Cancer Center, and Incyte Corporation. We are grateful for the ongoing efforts of study co-investigators and the HLH patients for whom they continue to provide care. A.A., S.A.M., and R.A.W. provided and interpreted data. P.S.B. and R.A.W. analyzed data and drafted the manuscript. All authors reviewed and approved the final manuscript. The authors declare no conflict of interest. The data are not publicly available due to privacy or ethical restrictions. Table S1. Baseline characteristics. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.