tbuckley/cabot-search huggingface dataset:
Hierarchical macro-microporous WPU-ECM scaffolds combined with Microfracture Promote in Situ Articular Cartilage Regeneration in Rabbits
Authors: Mingxue Chen, YangYang Li, Shuyun Liu, Zhaoxuan Feng, Hao Wang, Dejin Yang, Weimin Guo, Zhiguo Yuan, Shuang Gao, Yu Zhang, Kangkang Zha, Bo Huang, Fu Wei, Xinyu Sang, Qinyu Tian, Xuan Yang, Xiang sui, Yixin Zhou, Yufeng Zheng, Quanyi Guo
Journal: Bioactive Materials (2020)

Tissue engineering provides a promising avenue for treating cartilage defects. However, great challenges remain in the development of structurally and functionally optimized scaffolds for cartilage repair and regeneration. In this study, decellularized cartilage extracellular matrix (ECM) and waterborne polyurethane (WPU) were employed to construct WPU and WPU-ECM scaffolds by water-based 3D printing using low-temperature deposition manufacturing (LDM) system, which combines rapid deposition manufacturing with phase separation techniques. The scaffolds successfully achieved hierarchical macro-microporous structures. After adding ECM, WPU scaffolds were markedly optimized in terms of porosity, hydrophilia and bioactive components. Moreover, the optimized WPU-ECM scaffolds were found to be more suitable for cell distribution, adhesion, and proliferation than the WPU scaffolds. Most importantly, the WPU-ECM scaffold could facilitate the production of glycosaminoglycan (GAG) and collagen and the upregulation of cartilage-specific genes. These results indicated that the WPU-ECM scaffold with hierarchical macro-microporous structures could recreate a favorable microenvironment for cell adhesion, proliferation, differentiation, and ECM production. In vivo studies further revealed that the hierarchical macro-microporous WPU-ECM scaffold combined with the microfracture procedure successfully regenerated hyaline cartilage in a rabbit model. Six months after implantation, the repaired cartilage showed a similar histological structure and mechanical performance to that of normal cartilage. In conclusion, the hierarchical macro-microporous WPU-ECM scaffold may be a promising candidate for cartilage tissue engineering applications in the future.
HbA1c Change and Diabetic Retinopathy During GLP-1 Receptor Agonist Cardiovascular Outcome Trials: A Meta-analysis and Meta-regression
Authors: M. Angelyn Bethel, Rafael Diaz, Noelia Castellana, Indranil Bhattacharya, Hertzel C. Gerstein, Mark C. Lakshmanan
Journal: Diabetes Care (2020)

BACKGROUND Long-term glycemic control reduces retinopathy risk, but transient worsening can occur with glucose control intensification. Glucagon-like peptide 1 receptor agonists (GLP-1RA) lower glucose, but the long-term impact on retinopathy is unknown. GLP-1RA cardiovascular outcome trials (CVOTs) provide long-term follow-up, allowing examination of retinopathy outcomes. PURPOSE To examine the associations between retinopathy, HbA1c, systolic blood pressure (SBP), and weight in GLP-1RA CVOTs. DATA SOURCES Systematic review identified six placebo-controlled GLP-1RA CVOTs reporting prespecified retinopathy outcomes. STUDY SELECTION Published trial reports were used as the primary data sources. DATA EXTRACTION HbA1c, SBP, and weight data throughout follow-up by treatment group were extracted. DATA SYNTHESIS Random-effects model meta-analysis showed no association between GLP-1RA treatment and retinopathy (odds ratio [OR] 1.10; 95% CI 0.93, 1.30), with high heterogeneity between studies (I2 = 52.2%; Q statistic P = 0.063). Univariate meta-regression showed an association between retinopathy and average HbA1c reduction during the overall follow-up (slope = 0.77, P = 0.007), but no relationship for SBP or weight. Sensitivity analyses for HbA1c showed a relationship at 3 months (P = 0.006) and 1 year (P = 0.002). A 0.1% (1.09 mmol/mol) increase in HbA1c reduction was associated with 6%, 14%, or 8% increased Ln(OR) for retinopathy at the 3-month, 1-year, and overall follow-up, respectively. LIMITATIONS CVOTs were not powered to assess retinopathy outcomes and differed in retinopathy-related criteria and methodology. The median follow-up of 3.4 years is short compared with the onset of retinopathy. CONCLUSIONS HbA1c reduction was significantly associated with increased retinopathy risk in meta-regression for GLP-1RA CVOTs. The magnitude of HbA1c reduction was correlated with retinopathy risk in people with diabetes and additional cardiovascular risk factors, but the long-term impact of improved glycemic control on retinopathy was unmeasured in these studies. Retinopathy status should be assessed when intensifying glucose-lowering therapy.
Confection Confusion: Interplay Between Diet, Taste, and Nutrition
Authors: Christina E. May, Monica Dus
Journal: Trends in Endocrinology and Metabolism (2020)

Taste sensation varies with age, disease state, and environmental exposure. Emerging data from animal models suggest that diet composition, particularly the levels of sugar and fat, alter sweet taste sensation to influence feeding behavior and the risk of metabolic disease.Diet composition and obesity contribute to different aspects of chemosensory plasticity.Consumption of high sugar and/or high-fat diets reshapes the transcriptome and epigenome of the taste cells and nerves and dulls their responsiveness to stimuli.Sugar levels in the diet also affect taste sensation in humans, but more research is needed to understand the impact of high sugar intake on taste and feeding. Although genetics shapes our sense of taste to prefer some foods over others, taste sensation is plastic and changes with age, disease state, and nutrition. We have known for decades that diet composition can influence the way we perceive foods, but many questions remain unanswered, particularly regarding the effects of chemosensory plasticity on feeding behavior. Here, we review recent evidence on the effects of high-nutrient diets, especially high dietary sugar, on sweet taste in vinegar flies, rodents, and humans, and discuss open questions about molecular and neural mechanisms and research priorities. We also consider ways in which diet-dependent chemosensory plasticity may influence food intake and play a role in the etiology of obesity and metabolic disease. Understanding the interplay between nutrition, taste sensation, and feeding will help us define the role of the food environment in mediating chronic disease and design better public health strategies to combat it. Although genetics shapes our sense of taste to prefer some foods over others, taste sensation is plastic and changes with age, disease state, and nutrition. We have known for decades that diet composition can influence the way we perceive foods, but many questions remain unanswered, particularly regarding the effects of chemosensory plasticity on feeding behavior. Here, we review recent evidence on the effects of high-nutrient diets, especially high dietary sugar, on sweet taste in vinegar flies, rodents, and humans, and discuss open questions about molecular and neural mechanisms and research priorities. We also consider ways in which diet-dependent chemosensory plasticity may influence food intake and play a role in the etiology of obesity and metabolic disease. Understanding the interplay between nutrition, taste sensation, and feeding will help us define the role of the food environment in mediating chronic disease and design better public health strategies to combat it.
Psychological impact of COVID-19 and implementability of prevention and control measures in hemodialysis centers: A provincial questionnaire
Authors: X. Liang, Y. Chen, Y. Wu, P. Hu
Journal: Journal of the American Society of Nephrology (2020)

Background: This study investigated the psychological status of patients and staff and implementabilty of prevention measures in hemodialysis centers in Guangdong Province of China during the Coronavirus disease 2019 (COVID-19) pandemic  Methods: An electronic questionnaire survey was performed in an anonymous manner between March 28 and April 3, 2020  Two questionnaires were designed to investigate the psychological status for hemodialysis patients and general staff (doctors, nurses, technicians, and other staff), respectively  And an additional questionnaire for administrators (directors or head nurses) of hemodialysis centers was designed to address the implementabilty of prevention measures, including strengthened patient triage management, restricting caregiver visits to patients during dialysis, strengthened prevention amongst staff, and improved patient education and protection  All the 516 hemodialysis centers registered in Guangdong Province were voluntarily invited to join the survey  Results: Total 1,782 patients, 3,400 staff, and 420 administrators responded for this survey  Patients living in rural areas reported a higher incidence of severe anxiety compared to those living in other areas  Medical staff reported better mental health than non-medical staff  With respect to implementabilty of prevention measures, hemodialysis centers in general hospitals outperformed independent blood purification centers, and tertiary hospitals outperformed other level hospitals  However, restricting acceptance of non-resident patients was lower in tertiary hospitals than that in other hospitals  Under this condition, only one patient imported from Hubei Province was diagnosed with COVID-19  Conclusions: The outbreak did not significantly affect the psychological status of most patients and medical staff  Due to the successful implementation of comprehensive prevention measures, the COVID-19 epidemic was controlled effectively  This provincial survey may provide experience for other countries and regions with similar epidemic
A fluorescence sandwich immunoassay for the real-time continuous detection of glucose and insulin in live animals
Authors: Mahla Poudineh, Caitlin L. Maikawa, Eric Yue Ma, Jing Pan, Dan Mamerow, Yan Hang, Sam W. Baker, Ahmad Beirami, Alex Yoshikawa, Michael Eisenstein, Seung Kim, Jelena Vučković, Eric A. Appel, H. Tom Soh
Journal: Nature Biomedical Engineering (2020)
Clinical Symptoms in 44 Hemodialysis Patients Who Survived and Recovered from a Confirmed SARS-CoV-2 Infection and COVID-19 in Relation to Age and Hospitalization: An International Experience
Authors: Mauricio Ruiz, Szymon Brzosko, Marta Serwanska-Swietek, Werner Kleophas, Thilo Krueger, Joao M. Frazao, Fatima F. Silva, Wisam H. Al-Badr, Atef Z. Altaher, Rosnawati Yahya, Maciej Drozdz, Partha Das, Claudio A. Monteiro, Stefan H. Jacobson
Journal: Journal of the American Society of Nephrology (2020)

Background: A novel coronavirus (SARS-CoV-2) is now rapidly spreading throughout the world  Patients undergoing long-term in-center hemodialysis (HD) are highly vulnerable given kidney failure, comorbidities, and the need for frequent visits to a dialysis facility  Methods: A total of 610 patients on maintenance HD at DaVita clinics in 6 countries were tested for presence of infection with SARS-CoV-2 using polymerase chain reaction (PCR) between March 28 and May 18  Of these, 115 HD patients (19%) were positive  Information up to May 25, 2020, show that 44 patients have recovered  Clinical symptoms during infection with SARS-CoV-2 are reported from these 44 recovering survivors (Germany 13 patients, Poland 12, Portugal 12, Colombia 2, Saudi Arabia 3, and Malaysia 2) and classified into 4 categories: no symptoms, mild, moderate, or severe symptoms  Hospitalizations and time to recovery were also analyzed  Statistical comparisons were made using Chi-2 analysis and Kruskal-Wallis tests  Results: Of the 44 patients recovering from COVID-19, 22 patients were ≥70 years and 9 patients were >;80 years  Symptoms in relation to age, hospitalization, and time to recovery are shown below  Conclusions: The majority of HD patients (66%) who recovered from COVID-19 had no or mild clinical symptoms during the infection  There were no significant differences in the occurrence of symptoms from SARS-CoV-2 in relation to age, hospitalization, or time to recovery  Additionally, old and frail HD patients with confirmed COVID-19 may have mild symptoms of the disease
AKI due to COVID-19 in the Intensive Care Unit: Analysis of a Brazilian Center
Authors: Precil D. Neves, Victor Sato, Sara Mohrbacher, Bernadete Ferreira, Leonardo V. Pereira, Erico S. Oliveira, Alessandra M. Bales, Luciana L. Nardotto, Jéssica Ferreira, David Machado, Estevao Bassi, Amilton Silva-Junior, Pedro Chocair, Americo L. Cuvello neto
Journal: Journal of the American Society of Nephrology (2020)

Neves, Precil D.; Sato, Victor; Mohrbacher, Sara; Ferreira, Bernadete; Pereira, Leonardo V.; Oliveira, Erico S.; Bales, Alessandra M.; Nardotto, Luciana L.; Ferreira, Jéssica; Machado, David; Bassi, Estevao; Silva-Junior, Amilton; Chocair, Pedro; Cuvello neto, Americo L. Author Information
Correction: HER2-Overexpressing Breast Cancers Amplify FGFR Signaling upon Acquisition of Resistance to Dual Therapeutic Blockade of HER2
Authors: Ariella B. Hanker, Joan T. Garrett, Mónica Valeria Estrada, Preston D. Moore, Paula González Ericsson, James P. Koch, Emma Langley, Sharat Singh, Phillip S. Kim, Garrett M. Frampton, Eric Sanford, Philip Owens, Jennifer Becker, M. Reid Groseclose, Stephen Castellino, Heikki Joensuu, Jens Huober, Jan C. Brase, Samira Majjaj, Sylvain Brohée, David Venet, David Brown, José Baselga, Martine Piccart, Christos Sotiriou, Carlos L. Arteaga
Journal: Clinical Cancer Research (2019)

In the original version of this article (1), the stated disclosure of the author Carlos L. Arteaga is incorrect. The error has been corrected in the latest online HTML and PDF versions of the article. The authors regret this error.
Pulmonary granulomas: give it a whorl
Authors: Li-Ta Keng, Wei-Yung Lo, Hsien-Neng Huang, Huan-Jang Ko, Sheng-Kai Liang
Journal: Thorax (2020)

A 60-year-old woman with systemic lupus erythematosus, diabetes mellitus and chronic kidney disease presented to the respiratory clinic for shortness of breath on exertion for 6 months and an abnormal routine chest radiography obtained 1 year earlier. She did not have cough, sputum or chest pain. On examination, she was alert and oriented without distress. Auscultation revealed bilateral expiratory wheezes and normal heart sounds without murmur. Her oxygenation was 96% while she was breathing ambient air. Chest radiograph (figure 1A) and CT of the chest (figure 1B) showed bilateral, multiple, variable-sized pulmonary nodules and masses. The lesions were round or polygonal in shape, non-enhancing after contrast injection, with right lower lobe predominance (figure 1C). She had been told that a routine chest radiography 1 year earlier showed multiple pulmonary nodules. Pulmonary function test revealed moderate obstructive ventilator defect with …
Extracorporeal Cytokine Reduction Using Oxiris Blood Purification in COVID-19
Authors: Spencer Hodgins, Marat Abdullin, Mark A. Tidswell, Daniel L. Landry
Journal: Journal of the American Society of Nephrology (2020)

Introduction: Severe COVID-19 infection can cause 'cytokine storm' and endorgan dysfunction  OXIRIS, a blood-purification filter, was recently approved by the FDA under emergency use authorization for this indication due to its ability to remove cytokines and endotoxin through its AN-69ST membrane  We describe our experience with the first two cases treated at our institution  Case Description: Case 1: 58 year-old female patient with a baseline creatinine of 0 8 mg/dL & history of hemoglobin SC disease was admitted with respiratory failure due to COVID-19 infection  She deteriorated on hospital day (HD) 6 and was intubated  She received broad-spectrum antibiotics and convalescent plasma  On HD 15 she had increasing vasopressor requirement, anuric AKI and was started on continuous renal replacement therapy (CRRT)  Due to worsening clinical status on HD18 she was started on the OXIRIS hemofilter through the CRRT circuit for 48 hours  Oxygenation improved and there was some improvement in inflammatory markers (IM) (table 1), however, the family withdrew care on HD 20  Case 2: 29-year-old male with no prior past medical history apart from morbid obesity presented with fever and dry cough in the setting of recent COVID-19 exposure  He was found to be COVID-19 positive and rapidly deteriorated with resultant intubation on HD4  He received hydroxychloroquine, doxycycline, remdesivir and convalescent plasma  OXIRIS hemoperfusion was initiated on HD8 due to worsening hypoxia despite high FiO2  Oxygenation improved by HD10 (table 1) and he was successfully extubated on HD16  Discussion: We present our first 2 cases using the OXIRIS hemofilter  We treated the patients for 48 hours with a scheduled filter exchange at 24 hours  We used a blood flow of 250 ml/min and dialysate flow of 25 ml/kg/hour with either systemic heparin or regional citrate anticoagulation along with 1L/hr of pre-filter saline  For hemoperfusion we used the same parameters without dialysate  We observed rapid improvements in oxygenation (Figure 1)  The findings are hypothesis generating though more data is needed to determine optimal timing and efficacy of this filter
AKI in Patients with COVID-19 Infection: Preliminary Data from AKI COVID-19 Registry of the Spanish Society of Nephrology
Authors: Maria Jose Soler, Esteban Poch, Raquel Diaz Mancebo, Rocĭo E. Echarri, Nuria Areste, Mercedes Salgueira Lazo
Journal: Journal of the American Society of Nephrology (2020)

Background: SARS-CoV-2 coronavirus pandemic has significant impact on the general population, and chronic hemodialysis patients presented a poor prognosis with a mortality rate around 25%  Data from severe acute kidney injury(AKI) and acute renal replacement therapy(RRT) is scarce  We present the preliminary results of AKI COVID-19 Registry of the Spanish Society of Nephrology  Methods: The online Registry began operating on May 21th  It collects epidemiological variables, contagion and diagnosis data, signs and symptoms, treatments and outcomes  Patients were diagnosed with SARS-Cov-2 infection based on PCR of the virus  Results: One week after the AKI COVID registry started, 54 patients with AKI with RRT and COVID-19 from 11 Hospitals  Age was 64+9years and 55% men  65% hypertension, 31% diabetes mellitus, 14% cardiovascular disease, 26% chronic kidney disease, 6% neoplasm, 29% obesity, 8% chronic obstructive pulmonary disease, and6% smokers  Previous treatment: 10% immunosuppressive, 20% ACEi, 25% ARBs, 14% antiplatelets, and 10% anticoagulants  Clinical characteristics: 92% common respiratory symptoms, 96% pneumonia, 90% required intensive care unit(ICU) and 87% mechanic ventilation  32% albuminuria, 18% hematuria, and 50% AKI with preserved urine output  Time from COVID-19 symptoms start to AKI 12 3+8days, time ICU 19 8+5days  APACHE at UCI admission 15+7  81% lymphopenia  RRT was needed in 91% 13 4+12days: 55% received continuous RRT, and 72% anticoagulation  Kidney biopsy was not performed  Mortality 46 3% (60% males), and 4% remained under RRT  Time from AKI to renal function recovery 25+14 days  65 2% death patients had hypertension  No differences were observed in comorbidities, chronic treatments, renal clinical characteristics, dialysis modality and mortality  Decreased lymphocyte count was associated with worse patient prognosis (dead 495±260 vs  survivors 789±460,p=0 023)  Conclusions: The mortality in AKI with RRT and COVID-19 is alarming high  Severe AKI associated with COVID-19 disease is more frequent in males  Interestingly, half of the patients preserved urine output  Severe lymphopenia was associated with mortality  More data from the AKI COVID-19 registry will help us to enlighten the prognosis and risk factors associated to mortality
三酸化ヒ素による、bakの調節とKip1/p27発現によるアポトーシスとHTLV-1感染T細胞に対するG(1)フェーズでの細胞周期停止の誘発
Authors: Sihitsuka K, Kitazono M, Hanada S, Suzuki S, Utsunomiya A, Takeshita T, Shimotakahara K, Uozumi K, Akiyama S, Arima T
Journal: Blood (1998)
ACCELERATE: A Patient-Powered Natural History Study Design Enabling Clinical and Therapeutic Discoveries in a Rare Disorder
Authors: Sheila K. Pierson, Johnson S. Khor, Jasira Ziglar, Amy Liu, Katherine Floess, Erin NaPier, Alexander M. Gorzewski, Mark-Avery Tamakloe, Victoria Powers, Faizaan Akhter, Eric Haljasmaa, Raj Jayanthan, Arthur Rubenstein, Mileva Repasky, Kojo Elenitoba-Johnson, Jason Ruth, Bette Jacobs, Matthew Streetly, Linus Angenendt, Jose Luis Patier, Simone Ferrero, Pier Luigi Zinzani, Louis Terriou, Corey Casper, Elaine Jaffe, Christian Hoffmann, Eric Oksenhendler, Alexander Fosså, Gordan Srkalovic, Amy Chadburn, Thomas S. Uldrick, Megan Lim, Frits van Rhee, David C. Fajgenbaum
Journal: Cell Reports Medicine (2020)

Geographically dispersed patients, inconsistent treatment tracking, and limited infrastructure slow research for many orphan diseases. We assess the feasibility of a patient-powered study design to overcome these challenges for Castleman disease, a rare hematologic disorder. Here, we report initial results from the ACCELERATE natural history registry. ACCELERATE includes a traditional physician-reported arm and a patient-powered arm, which enables patients to directly contribute medical data and biospecimens. This study design enables successful enrollment, with the 5-year minimum enrollment goal being met in 2 years. A median of 683 clinical, laboratory, and imaging data elements are captured per patient in the patient-powered arm compared with 37 in the physician-reported arm. These data reveal subgrouping characteristics, identify off-label treatments, support treatment guidelines, and are used in 17 clinical and translational studies. This feasibility study demonstrates that the direct-to-patient design is effective for collecting natural history data and biospecimens, tracking therapies, and providing critical research infrastructure.
Lung Ultrasound to Monitor Extremely Preterm Infants and Predict Bronchopulmonary Dysplasia. A Multicenter Longitudinal Cohort Study
Authors: Barbara Loi, Giulia Vigo, Eugenio Baraldi, Francesco Raimondi, Virgilio P. Carnielli, Fabio Mosca, Daniele De Luca
Journal: American Journal of Respiratory and Critical Care Medicine (2020)

Rationale: Lung ultrasound is useful in critically ill patients with acute respiratory failure. Given its characteristics, it could also be useful in extremely preterm infants with evolving chronic respiratory failure, as we lack accurate imaging tools to monitor them. Objectives: To verify if lung ultrasound can monitor lung aeration and function and has good reliability to predict bronchopulmonary dysplasia in extremely preterm neonates. Methods: A multicenter, international, prospective, longitudinal, cohort, diagnostic accuracy study consecutively enrolling inborn neonates with gestational age 30+6 weeks or younger. Lung ultrasound was performed on the 1, 7, 14, and 28 days of life, and lung ultrasound scores were calculated and correlated with simultaneous blood gases and work of breathing score. Gestational age–adjusted lung ultrasound scores were created, verified in multivariate models, and subjected to receiver operator characteristics (ROC) analyses to predict bronchopulmonary dysplasia at 36 weeks postmenstrual age. Measurements and Main Results: Mean lung ultrasound scores are different between infants developing (n = 72) or not developing (n = 75) bronchopulmonary dysplasia (P < 0.001 at any time point). Lung ultrasound scores significantly correlate with oxygenation metrics and work of breathing at any time point (P always < 0.0001). Gestational age–adjusted lung ultrasound scores significantly predict bronchopulmonary dysplasia at 7 (area under ROC curve, 0.826–0.833; P < 0.0001) and 14 (area under ROC curve, 0.834–0.858; P < 0.0001) days of life. Bronchopulmonary dysplasia severity and gestational age–adjusted lung ultrasound scores are significantly correlated at 7 and 14 days (P always < 0.0001). Conclusions: Lung ultrasound scores allow monitoring of lung aeration and function in extremely preterm infants. Gestational age–adjusted scores significantly predict the occurrence of bronchopulmonary dysplasia, starting from the seventh day of life.