tbuckley/cabot-search huggingface dataset:
Combination Treatment with All-Trans Retinoic Acid Prevents Cisplatin-Induced Enrichment of CD133+ Tumor-Initiating Cells and Reveals Heterogeneity of Cancer Stem Cell Compartment in Lung Cancer
Authors: Massimo Moro, Giulia Bertolini, Ugo Pastorino, Luca Roz, Gabriella Sozzi
Journal: Journal of Thoracic Oncology (2015)

The existence of specific cellular subpopulations within primary tumors with increased tumorigenic potential and chemotherapy resistance (tumor-initiating cells, TICs) holds great therapeutic implications. Resistant cells can remain quiescent for long periods and be responsible for local relapses and metastasis. We and others have previously described in non-small-cell lung cancer the presence of cisplatin-resistant CD133⁺ cells with tumor-initiating potential and co-expression of CXCR4 as possible indicator of TICs with disseminating potential. In this study, we report, by in vitro cell fate tracing systems, heterogeneity within the TIC compartment with a highly quiescent pool and a slowly dividing subpopulation, both containing CD133⁺ cells but respectively enriched for CD133⁺/CXCR4⁻ and CD133⁺/CXCR4⁺ cells. Pretreatment with differentiating agent all-trans retinoic acid counteracts cisplatin resistance specifically of the slowly dividing compartment indicating effect on CD133⁺/CXCR4⁺ cells. The same effects are appreciable also in vivo in patient-derived xenografts, where several cycles of all-trans retinoic acid and cisplatin treatment are able to stably reduce this fraction of TICs and tumor dissemination. Thus, partially affecting the heterogeneous TICs compartment, differentiating therapy has promising effects in counteracting cisplatin resistance of CD133⁺ cells, reducing both local tumor growth and dissemination. In addition, our approach discloses a further level of complexity of chemotherapy-resistant CD133⁺ TICs, revealing phenotypical and functional heterogeneity of the cancer stem cell compartment in lung cancer.
Abstract 18648: Heart Healthy Living: A Community-Based, Tailored Educational Program to Improve the Cardiovascular Knowledge and Health of African American Women
Authors: Aileen Sauris, Patricia Timmons, Fatima Rodriguez, Yun Wang, JoAnne M Foody
Journal: Circulation (2013)

Background: Low health literacy is a potential contributor to poor cardiovascular outcomes. Health literacy is lowest among the elderly, racial/ethnic minorities, those with low education level, the uninsured, and the publicly insured. African American (AA) women suffer from a disproportionate burden of CV risk factors (RF) and low health literacy. We developed a community-based, tailored health education, nutrition, and exercise program, Heart Healthy Living (HHL) to address the unique challenges in AA women. Methods: HHL consisted of a 6-week program to improve the CV health literacy of AA women aged 18 years or older with at least one CV risk factor. The program consisted of weekly tailored CV health education and exercise classes. In weeks 1 and 6, participants completed a validated Heart Disease Knowledge Questionnaire, blood pressure and weight were checked. Results: Participants included 32 AA women with an average age of48 years. Participants had high rates of hypertension (55%) and obesity (68%). Participants experienced an age-adjusted weekly weight loss of 0.5 lbs and an age-adjusted total weight loss of 2.7 lbs (p&lt;0.001). There was a 40% relative decline in rates of HTN after completion of the program. At baseline, participants scored &lt;50% on the validated Heart Disease Knowledge Questionnaire; however after completion of the program this improved significantly (Figure). Conclusion: We found that a tailored health education and exercise program resulted in improved CV health literacy in AA Women. Our data suggests an intensive CV education and exercise program, improved health literacy disparities and CV RF in AA women. Larger and longer studies of HHL are ongoing to determine whether these results are replicable and sustainable.
339 Chemoprevention of hepatocellular carcinoma. Assessment of the efficacy of pioglitazone and lanreotide in a carcinogenic rat model
Authors: Ivan Borbath, Valérie Lebrun, Jorge Abarca-Quinones, P. Moulin, Christine Sempoux, Isabelle Leclercq, Yves Horsmans
Journal: Journal of Hepatology (2005)
Cybord Is An Active, Well Tolerated, Cost-Effective Induction Regimen In Newly Diagnosed Multiple Myeloma – a Single Centre Experience
Authors: Janusz Krawczyk, Sahar Khan, Bushra Ahsan, Larissa Higgins, Enda McGowan, Safa Eltom, Khalid Awad Saeed, Carmelita Gibbons, Karen Maloney, Teresa Meenaghan, Geraldine Walpole, Catriona Collins, Robert Snedker, Moutaz Abdelrahmen, Amjad Hayat, Andrew Hodgson, Margaret Murray, Michael E O'Dwyer
Journal: Blood (2013)
Cohort study of a campylobacteriosis outbreak associated with chicken liver parfait, United Kingdom, June 2010
Authors: T Inns, K Foster, R Gorton
Journal: Eurosurveillance (2010)

In an outbreak of 24 cases of gastroenteritis among guests at a wedding reception, 13 cases had confirmed Campylobacter infection. In a cohort study, univariate analysis revealed a strong association with consumption of chicken liver parfait: risk ratio (RR): 30.08, 95% confidence interval (CI): 4.34-208.44, p&lt;0.001, which remained after adjustment for potential confounders in a multivariable model: RR=27.8, 95% CI=3.9-199.7, p=0.001. These analyses strongly support the hypothesis that this outbreak was caused by the consumption of chicken liver parfait.
Abstract 10858: Post-Ischemic Contractile Dysfunction is Exacerbated in a Porcine Model of Metabolic Syndrome, but Ameliorated by Restricting Fatty Acid Availability
Authors: Janice Huang, Ya Xu, Li Lu, Bryan Bergman, Shuyu Ye, Clifford Greyson, Gregory G Schwartz
Journal: Circulation (2011)

Background: The poor prognosis of acute coronary syndrome (ACS) in patients with metabolic syndrome (Met Syn) is not fully explained by the extent of coronary atherosclerosis, suggesting that myoca...
Brain SPECT used to evaluate vasospasm after subarachnoid hemorrhage: correlation with angiography and transcranial Doppler.
Authors: JOSEPH RAJENDRAN, DAVID LEWIS, DAVID NEWELL, H. WINN
Journal: Clinical Nuclear Medicine (2001)

The primary objective of this study was to correlate Tc-99m HMPAO and ethyl cysteine dimer perfusion brain SPECT imaging with angiography and transcranial Doppler (TCD) to identify vasospasm after subarachnoid hemorrhage.A retrospective analysis of consecutive patients who had cerebral blood flow SPECT imaging for subarachnoid hemorrhage and aneurysm clipping was made. Flow velocity measurements were correlated using TCD and cerebrovascular angiography.Of the 129 patients were included in this study, 84 were female and 45 were male, with a mean age of 51.9 years and a median age of 51 years (range, 9 to 84 years). Eighty-nine patients had brain SPECT evidence of hypoperfusion. Concordance was found between SPECT and TCD with vasospasm in 57 of 89 (64%) patients and nonconcordance was evident in 32 patients (36%). Eleven patients who had concordance between SPECT and TCD had nonconcordant results of angiography for vasospasm.These findings suggest that all three methods are complementary to each other in the evaluation of patients with vasospasm after subarachnoid hemorrhage. Concordance of 64% between SPECT and TCD is acceptable and explicable by the differences in technique and measurement of cerebral blood flow compared with vascular narrowing, respectively.
Abstract 1767: Feasibility of <i>In Vivo</i> Cardiac Stem Cell Tracking, by SPECT and PET Imaging, Using the Sodium-iodide Symporter as Reporter Gene
Authors: John Terrovitis, Keng Fai Kwok, Riikka Läutamaki, James M Engles, Andreas S Barth, Junichiro Miake, Michelle K Leppo, James Fox, Jurgen Seidel, Richard L Wahl, Benjamin Tsui, Frank Bengel, Eduardo Marbán, M. Roselle Abraham
Journal: Circulation (2007)

Background. Stem cells offer the promise of cardiac repair. Stem cell labeling is a prerequisite to tracking cell fate in vivo . Aim. To develop a reporter gene that permits in vivo stem cell labeling. We examined the sodium-iodide symporter (NIS), a protein that is not expressed in the heart, but promotes cellular uptake of 99m Tc or 124 I, thus permitting cell tracking by SPECT or PET imaging, respectively. Methods. The human NIS gene ( h NIS) was expressed in rat cardiac derived stem cells (rCDCs) using lentivirus driven by the CAG or CMV promoter. NIS function in transduced cells was confirmed by in vitro 99m Tc uptake. Eleven rats were injected with 1 or 2 million rCDCs intramyocardially immediately after LAD ligation; 6 with CMV-NIS and 5 with CAG-NIS cells. Dual isotope SPECT imaging was performed on a small animal SPECT/CT system, using 99m Tc for cell detection and 201 Tl for myocardial delineation, 24 hrs after cell injection. PET was performed on a small animal PET scanner using 124 I for cell tracking and 13 NH 3 for myocardial delineation, 48hrs after cell injection. Contrast Ratio (CR) was defined as [(signal in the cells)-(signal in blood pool)]/signal in blood pool. High resolution ex vivo SPECT scans of explanted hearts (n=3) were obtained to confirm that in vivo signal was derived from the cell injection site. The presence of h NIS mRNA was confirmed in injected hearts after animal sacrifice (n=2), by real-time RT-PCR. Results. NIS expression in rCDCs did not affect cell viability/proliferation (p=0.718, ctr vs NIS). In vitro 99m Tc uptake was 6.0±0.9% vs 0.07±0.05, without and with perchlorate (specific NIS blocker), respectively. NIS-transduced rCDCs were easily visualized as spots of 99m Tc or 124 I uptake within a perfusion deficit in the SPECT and PET images. CR was considerably higher when cells were transduced by the CMV-NIS virus in comparison to the CAG-NIS virus (70±40% vs 28±29%, p=0.085). Ex vivo small animal SPECT imaging confirmed that in vivo 99m Tc signals were localized to the injection sites. PCR confirmed the presence of h NIS mRNA in injected hearts. Conclusion. NIS expression allows non invasive in vivo stem cell tracking in the myocardium, using both SPECT and PET. This reporter gene has great potential for translation in future clinical applications.
FLT3, NPM1 and CEBPA Mutations in 195 Children with Acute Myeloid Leukemia in Argentina
Authors: Cristina N. Alonso, Patricia L. Rubio, Adriana Medina, Silvia Eandi Eberle, Andrea Bernasconi, Mariela Cocce, Marta Gallego, Pedro Zubizarreta, Jorge Gabriel Rossi, Maria Sara Felice
Journal: Blood (2014)
Abstract 18350: A Composite Risk Score of Biomarkers of Inflammation, Thrombosis, and Cell Stress Pathways Significantly Predict Risk of Adverse Cardiovascular Outcomes
Authors: Nima Ghasemzadeh, Hatem Al Kassem, Danny Eapen, Mohamed Khayata, Ayman S Tahhan, Pankaj Manocha, Bushra Alzaraneh, Ravi Nanjundappa, Chrsitian W Thorball, Riyaz Patel, Stamatios Lerakis, Laurence Sperling, Tomasz Pielak, Sergey Sikora, Stephen E Epstein, Arshed A Quyyumi
Journal: Circulation (2013)

Background: An aggregate biomarker risk score of C-reactive protein (CRP), heat shock protein (HSP)-70, and fibrin degradation products (FDP) significantly predicts adverse outcomes in patients with CAD. Soluble Urokinase Plasminogen Activator Receptor (suPAR) is an inflammatory biomarker that has been associated with incident CVD. We sought to investigate the value of adding plasma suPAR level to the 3 original biomarkers in predicting risk of adverse outcomes. Methods: 3,280 patients (aged: 62± 11, 83% white, 32% diabetics, 64% with CAD ≥50%) undergoing diagnostic angiography were followed for death and/or MI over median 2.3 yrs. Plasma suPAR (ViroGates, Denmark), and serum CRP, HSP-70, and FDP (Firstmark Inc) were measured using ELISA. Severity of CAD was assessed by the Gensini score. Cox proportional survival and C-statistic analyses were performed adjusting for traditional risk factors, ejection fraction, serum creatinine, and Gensini. Results: Patients with suPAR level ≥ 3.5ng/ml (ROC cutoff) had a greater CAD burden (Gensini: 49± 68 vs. 41± 64, p=0.003). Plasma suPAR ≥ 3.5 also predicted future death/MI (HR=1.76, p&lt;0.001) after adjustment for all covariates and the 3 biomarkers, CRP, HSP-70, and FDP; the C-statistic improved (0.69 to 0.71, p=0.01). A 4 biomarker aggregate score composed of suPAR, CRP, FDP, and HSP-70 below/above cutoffs significantly predicted risk of death/MI. Thus, compared to those with 0 biomarkers, HR of death/MI for those with 1, 2, 3, or 4 positive biomarkers were: 1.5, 2.1, 4.4, and 7.3 respectively (P&lt;0.001). Addition of the 4-marker score significantly improved the C-statistic compared to a model of traditional factors (0.68 to 0.75, p=0.004). Conclusion: An aggregate risk score of CRP, HSP-70, FDP, and suPAR representing inflammatory, thrombotic, and cell stress pathways, identifies CAD patients at low and very high risk of death/MI. Thus, the score allows individualization of risk in CAD patients.
Beta-blocker therapy nor hyperoxic breathing lowers ventilatory drive in pulmonary arterial hypertension patients
Authors: Herman Groepenhoff, Jasmijn van Campen, Anco Boonsta, Anton Vonk-Noordegraaf, Harm Jan Bogaard
Journal: European Respiratory Journal (2014)

Introduction:  Hypocapina at rest and during exercise is a common finding in pulmonary arterial hypertension patients (PAH). Increased dead-space ventilation, due to decreased perfusion of the lung, enhances ventilatory demand in PAH patients but does not explain hypocapnia. It has been suggested that increased sympathetic activation and hypoxemia play a role in driving ventilation in PAH.  In this study we tested the hypothesises that reduced sympathetic activation by beta-blocker therapy or increased inspiratory oxygen pressure will lower ventilatory drive in patients with PAH.   Methods:  We measured in 14 stable PAH patients, at baseline, minute ventilation (VE), arterial carbon dioxide pressure (PaCO 2 ), heart rate (HR) and the linear regression slope relating ventilation to carbon dioxide production during exercise (VE/VCO 2 ). VE and HR were also measured during breathing a gas mixture with an inspiratory oxygen fraction of 40%. After three months of treatment of placebo or the beta-blocker bisoprolol (double blind cross-over study design), all measurements were repeated while breathing room air only. Mixed linear regression analysis was used to correct for confounding factors and to check for significance.   Results:    Δ: absolute difference  , *** p l 0.0001 vs HR  (paired t-test)    Conclusion:  Neither beta-blocker therapy nor hyperoxic gas breathing lowers ventilatory drive in PAH patients.
Reconceptualising placebo
Authors: G Lorimer Moseley
Journal: BMJ (2008)

Four patients with salt-losing congenital adrenal hyperplasia (CAH) who had stopped mineralocorticoid therapy for several years, showed raised plasma concentrations of 17OH-progesterone and plasma renin activity, despite adequate glucoticoid therapy. One patient was able to reduce urinary sodium excretion when the sodium intake was restricted. Another patient who was a salt-loser, developed signs of an adrenal crisis when salt deprived. In comparison, one nonsalt-loser and 2 normal subjects decreased urinary sodium excretion in response to sodium restriction. The addition of fludrocortisone (100 micrograms) to usual maintenance doses of glucocorticoid, resulted in normal levels of plasma 17OH-progesterone and plasma renin activity in all 4 salt-losers. Two female salt-losers, with raised plasma testosterone concentrations, began menstruating when their plasma testosterone concentrations returned to normal after treatment with fludrocortisone. It is recommended that salt-losing CAH patients should be given mineralocorticoid, in addition to glucocorticoid therapy, at least until adult life.
Abstract 5898: ADAMTS-1 is an Endothelial Cell-specific Hypoxia-inducible Gene
Authors: Satoshi Hirohata, Faruk O Hatipoglu, Toru Miyoshi, Hiroko Ogawa, Masanari Obika, Shigeshi Kamikawa, Shozo Kusachi, Hiroshi Itoh, Yoshifumi Ninomiya
Journal: Circulation (2009)

Background: We have previously reported that ADAMTS1 (A disintegrin and metalloprotease with thrombospondin motifs), a member of the metalloproteinase family, is induced in the infarcted heart in r...
Effect of recombinant human erythropoietin combined with granulocyte/ macrophage colony-stimulating factor in the treatment of patients with myelodysplastic syndrome
Authors: John A. Thompson, D. Gary Gilliland, Josef T. Prchal, John M. Bennett, Kay Larholt, Richard A. Nelson, Esther H. Rose, Margaret H. Dugan
Journal: Blood (2000)
Abstract 17937: Higher Parity is Associated with Left Ventricular Diastolic Dysfunction: Echo Study of Latinos (Echo SOL)
Authors: Shivani R Aggarwal, David M Herrington, Catherine J Vladutiu, Katrina Swett, Carlos J Rodriguez
Journal: Circulation (2014)

Introduction:  Previous literature indicates that having six or more pregnancies is associated with an increased risk of cardiovascular disease and that diastolic dysfunction (DD) may persist post-delivery. However, this relationship has not been studied in a Hispanic/Latina population despite higher parity (i.e., number of prior live births) observed amongst them. Our objective was to examine the relationship between parity and diastolic function in a population-based cohort. We hypothesized that higher parity would be associated with DD.   Methods:  Participants included 855 Hispanic/Latina women, aged 45 and older, who were enrolled in the population-based Echocardiographic Study of Latinos (Echo-SOL) from four communities across the U.S. (Bronx, Miami, San Diego, and Chicago) and representative of several Hispanic backgrounds. Wake Forest School of Medicine served as the centralized reading center. Reproductive history was obtained by questionaire and DD was graded following an algorithm that combined ASE and Redfield criteria. Using sampling weights and survey statistics, multivariable logistic regression models assessed the relationship between parity and the presence of DD (grades I-III).   Results:  In the target population, 4.7% were nulliparous (no live births) and 12.2% were grand multiparous (≥5 live births); 25% had diabetes, 42% had pre-diabetes and 27% were on anti-hypertensive medications. Among the nulliparous women, 51% had DD as compared to 61-63% of women with 2-4 live births and 85% of women with ≥5 live births (p = 0.03). In a multivariable model adjusted for age, body mass index, diabetes, systolic blood pressure and use of anti-hypertensive medications, the odds ratio for any grade of DD in grand-multiparous women compared to nulliparous women was 3.15 (95% CI 1.20-8.24, p   Conclusions:  Increasing number of live births is associated with the presence of DD among Hispanic/Latina women. Further studies are needed to determine the pathophysiology for the apparent deleterious consequences on diastolic function and whether these changes translate into heart failure with preserved ejection fraction.
Abstract 556: Cardiomyocytes from Human Embryonic Stem Cells Using an Efficient Directed Differentiation Method: In Vitro Characterization and Transplantation
Authors: Mohammad Hassanipour, Chunhui Xu, Chris O'Sullivan, Yinhong Chen, Catherine Priest, Lila Collins, Michael A Laflamme, Wei-Zhong Zhu, Joseph Gold
Journal: Circulation (2006)
Abstract 3438: Gene Expression Profiling of G Protein-Coupled Receptors in the Adult Mouse Heart
Authors: Thomas Moore-Morris, Annie Varrault, Matteo E Mangoni, Anne Le Digarcher, Vincent Negre, Laurent Journot, Brigitte Couette
Journal: Circulation (2008)

The G protein-coupled receptors (GPCRs), one of the major targets of pharmaceutical drugs, are essential to many aspects of heart physiology including the regulation of heart rate, contractile force, cardiac remodelling and gene expression. The present study addresses the expression of endoGPCRs, all GPCRs excluding taste and odorant receptors, in each of the four cardiac chambers of adult mouse heart. High-throughput real-time RT-PCR was used to quantify 396 GPCRs and 7 associated proteins in right and left atria and ventricles. Statistical analysis of the most highly expressed transcripts showed that 47 were equally expressed throughout the heart and 89 were differentially expressed in at least one cardiac chamber. Among receptors uniformly expressed, we found well-characterized receptors such as the β2 adrenergic receptor, muscarinic M2 receptor, endothelin receptor type A (ETA) and purinoceptor P2Y2. Surprisingly enough, many of the most abundantly expressed GPCRs (e.g. belonging to the frizzled or chemokine receptor subfamilies) had not previously been identified in healthy heart. Repertoire analysis revealed genes specific to atria and ventricles. The atria exhibited the most specific signature with a lot of receptors not yet studied (receptors belonging to the EGF-like, Mucin-like, Decay-accelerating Factor and Mas-related subfamilies). No gene appeared to be significantly specific to the left or right side of the heart. RT-PCR data were confirmed at the protein level in all four cavities for several receptors and regulatory proteins including ETA, angiotensin receptor 1, protease-activated receptor 1, chemokine receptor CCR2, secreted frizzled related protein 1 and metabotropic glutamate receptor 1 (mGluR1). We also demonstrate that the mGluR1b splice variant is the sole highly expressed metabotropic glutamate receptor in adult mouse heart and is present and functional in isolated ventricular cardiomyocytes. Thus, large scale identification of the regional expression pattern of GPCRs in normal, and in the future in pathological conditions, might lead to the identification of new pharmacological targets for the treatment of human heart diseases.
One Word
Authors: 和好 木下
Journal: JAMA (1995)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3068: Lesion Flow Coefficient: A Novel Diagnostic Index to Assess Coronary Occlusions Using Combined in vivo Measurements of Blood Flow, Pressure Gradient and Area Blockage
Authors: Abhijit Sinha Roy, Saeb F Khoury, Vijaya Velury, Eric W Schneeberger, Ronald W Millard, Rupak K Banerjee
Journal: Circulation (2006)