tbuckley/cabot-search huggingface dataset:
Endoscopic magnet-assisted gastrojejunostomy to treat symptoms caused by a deformed pylorus
Authors: Long He, Xiaobo Qin, Liansong Ye, Bing Hu
Journal: Endoscopy (2021)

A 42-year-old woman presented with a 5-year history of abdominal distension, abdominal pain, and emesis, which became worse after she began eating a little more. Gastroduodenal barium examination detected a deformed pylorus ([Fig. 1 a]). Subsequently, a gastroduodenoscopy was performed, which revealed that the outlet of her stomach lay on the lesser curvature side of the gastric angulus, with a blind end at the antrum ([Fig. 1 b]). We therefore suggested to the patient that she undergo surgery; however, she declined to undergo such treatment, preferring instead an endoscopic method to treat her problems. In the end, we decided to attempt, with the patient’s informed consent, an endoscopic magnet-assisted gastrojejunostomy ([Video 1]).
Abstract 9423: Virtual Reality (VR) Guided Telerobotic Cardiac Catheter Ablation is Feasible Over Low Bandwidth Network Connection
Authors: Stephen P Seslar, Ryan C James, Blake Hannaford, Basil Saour, Wayne Monsky
Journal: Circulation (2021)

Introduction: Telerobotic operation in which a remote surgeon performs an operation over distance has long held the promise of providing a more equitable distribution of healthcare in rural and underserved regions of the world. One of the technological limitations that has hampered the adoption of telerobotic surgery is the need for a broadband connection to transmit high-resolution video of the operative field. Hypothesis: We hypothesized that we could develop a low-bandwidth system for telerobotic cardiac catheter ablation that deploys a virtual reality (VR)-based surgical guidance system to allow remote operation without the need for high-resolution video transmission. Methods: We assembled a portable pre-clinical telerobotic catheter ablation system from commercial-grade components by 3 rd party vendors. Raw sensor data from the catheter ablation system requiring a bitrate of less than 100kb/sec was streamed in real-time over distance using a standard internet connection. We used a proprietary service (DOPL Technologies, Seattle, WA) and an Oculus Quest (Oculus, Inc. Menlo Park, CA) to generate a shared, virtual operative field and operating room environment. No video data was transmitted. The Institutional Animal Care and Use Committee of the University of Washington (Seattle, WA) approved the study performed on a 14-week (27 kg) Yorkshire pig. The operator was located 6 miles from the OR at the time of the operation. Results: A successful proof of concept telerobotic cardiac catheter ablation in an animal model was performed without video transmission. The remote surgeon applied radiofrequency energy lesions in the animal’s cavotricuspid isthmus region. The visual depiction of the operative environment was successfully constructed in virtual reality from sensor data generated from the animal subject and surgical instruments. The remote operator conducted the ablation while interacting in a shared virtual OR environment with members of the surgical team present in the physical operating room and three remote observers. Conclusions: Telerobotic cardiac catheter ablation is feasible without video transmission. This approach could reduce network bandwidth requirements for telerobotic operations in rural areas.
Risk and Severity of Cytokine Release Syndrome in Patients with Relapsed/Refractory (R/R) AML or MDS Treated with CD3xCD123 Bispecific Antibody APVO436
Authors: Tara L. Lin, Justin M Watts, Alice S. Mims, Prapti Patel, Paul J Shami, Elizabeth H. Cull, Christopher R. Cogle, Cynthia Lee, Fatih Uckun
Journal: Blood (2021)
Quantitative Change in Metabolic Tumor Volume May Assist in Distinguishing between Pseudoprogressors and Responders in Patients with Relapsed/Refractory Classical Hodgkin Lymphoma Treated with PD-1 Blockade
Authors: Beatriz Wills, Laure Michaud, Heiko Schöder, Nivetha Ganesan, Craig H. Moskowitz, David J. Straus, Lorenzo Falchi, Matthew J. Matasar, Andrew D. Zelenetz, Erel Joffe, Gottfried von Keudell, Connie Lee Batlevi, Audrey Hamilton, Philip C Caron, Ariela Noy, Gilles Salles, Alison J. Moskowitz
Journal: Blood (2021)
Global Proteomic Profiling Identifies Novel Prognostic Factors in Undifferentiated Leukemia Blasts from Patients with NPM1 Mutations: A Previously Unreported Approach to Biomarker Discovery from the Fred Hutch and SWOG
Authors: Derek Stirewalt, Megan Othus, Jasmine Naru, Jacob J Kennedy, Era L Pogosova-Agadjanyan, Frederick R. Appelbaum, Lan Beppu, Thomas R. Chauncey, Jeffrey Delrow, Harry P. Erba, Phillip R Gafken, John E. Godwin, Matthew Fitzgibbon, Xing Hua, Isaac C. Jenkins, Min Fang, Richard G Ivey, ChenWei Lin, Travis D Lorentzen, Anna Moseley, Jerald P. Radich, Uliana J Voytovich, Pei Wang, Jeffrey Whiteaker, Cheryl L Willman, Feinan Wu, Soheil Meshinchi, Amanda G Paulovich
Journal: Blood (2021)
The Gut Microbiota in Patients with Polycythemia Vera: Novel Vs. Conventional Treatments
Authors: Christina Schjellerup Schjellerup Eickhardt-Dalbøge, Anna Cäcilia Ingham, Lee O'Brien Andersen, Henrik V Nielsen, Kurt Fuursted, Morten Kranker Larsen, Lasse Kjær, Sarah Friis Christensen, Trine Alma Knudsen, Vibe Skov, Christina Ellervik, Hans C. Hasselbalch, Jens Jørgen Elmer Christensen, Xiaohui Chen Nielsen
Journal: Blood (2021)
Abstract PO-069: Modeling the tumor microenvironment using tissue engineering technologies
Authors: Rodrigo Curvello, Verena Kast, Daniela Loessner
Journal: Cancer Research (2021)

Abstract Tissue engineering technologies provide controllable and reproducible approaches to reconstruct the extracellular and cellular elements of pancreatic cancer. In a reductionist approach they allow the modelling of the complex tumor microenvironment (TME) and the study of disease biology and anti-cancer treatments. Our objective is that an engineered TME model, that mimics the tissue and matrix composition, architecture and cell types, will behave like a real tumor and is suitable for preclinical drug testing. Using biomimetic materials, we recreated the desmoplastic tissue characteristics of the pancreatic TME. Our natural and synthetic biomaterials were tailored to achieve the mechanical properties that resembled the stiffness and viscoelasticity of patient-derived tissues, providing a supportive cell-matrix interface for 3D cell culture conditions. Pancreatic cancer cells grown embedded in the matrix scaffolds formed tumor spheroids. Cell-based assays and microscopic analysis indicated a high cell viability and proliferation of the tumor spheroids as well as the expression of cancer-associated markers. Incorporation of cancer-associated fibroblasts and myeloid cells led to a multicellular 3D systems and matrix stiffening due to the secretion of extracellular matrix proteins. Transcriptomic analysis of the 3D cell cultures identified differentially regulated pathways related to cell proliferation, mechano-transduction and the secretion of pro-inflammatory cytokines, indicative of a malignant behavior. Treatment with mechano-modulating inhibitors and anti-cancer compounds increased the efficacy of chemotherapeutics, thereby reducing matrix stiffness and the release of cytokines. Our engineered TME model provides an easily translatable technology to ease the burden of pancreatic cancer, allowing us to characterize combination treatments that slow down or reduce tumor growth. Citation Format: Rodrigo Curvello, Verena Kast, Daniela Loessner. Modeling the tumor microenvironment using tissue engineering technologies [abstract]. In: Proceedings of the AACR Virtual Special Conference on Pancreatic Cancer; 2021 Sep 29-30. Philadelphia (PA): AACR; Cancer Res 2021;81(22 Suppl):Abstract nr PO-069.
An integrated workflow for enhanced taxonomic and functional coverage of the mouse fecal metaproteome
Authors: Nicolas Nalpas, Lesley Hoyles, Viktoria Anselm, Tariq Ganief, Laura Martinez-Gili, Cristina Grau, Irina Droste-Borel, Laetitia Davidovic, Xavier Altafaj, Marc-Emmanuel Dumas, Boris Macek
Journal: Gut Microbes (2021)

Intestinal microbiota plays a key role in shaping host homeostasis by regulating metabolism, immune responses and behavior. Its dysregulation has been associated with metabolic, immune and neuropsychiatric disorders and is accompanied by changes in bacterial metabolic regulation. Although proteomics is well suited for analysis of individual microbes, metaproteomics of fecal samples is challenging due to the physical structure of the sample, presence of contaminating host proteins and coexistence of hundreds of taxa. Furthermore, there is a lack of consensus regarding preparation of fecal samples, as well as downstream bioinformatic analyses following metaproteomics data acquisition. Here we assess sample preparation and data analysis strategies applied to mouse feces in a typical mass spectrometry-based metaproteomic experiment. We show that subtle changes in sample preparation protocols may influence interpretation of biological findings. Two-step database search strategies led to significant underestimation of false positive protein identifications. Unipept software provided the highest sensitivity and specificity in taxonomic annotation of the identified peptides of unknown origin. Comparison of matching metaproteome and metagenome data revealed a positive correlation between protein and gene abundances. Notably, nearly all functional categories of detected protein groups were differentially abundant in the metaproteome compared to what would be expected from the metagenome, highlighting the need to perform metaproteomics when studying complex microbiome samples.
<i>The ERK1/2 Regulator WNK2 Shows Differential Expression and Isoform Usage, Primarily Driven By Aberrant Methylation, in MYD88 Mutated Waldenström's Macroglobulinemia</i>
Authors: Maria Luisa Guerrera, Guang Yang, Xia Liu, Nickolas Tsakmaklis, Maria G Demos, Amanda Kofides, Manit Munshi, Lian Xu, Christopher J Patterson, Jorge J. Castillo, Shayna Sarosiek, Catherine A Flynn, Kirsten Meid, Joshua Gustine, Tomasz Sewastianik, Ruben D. Carrasco, Steven P Treon, Zachary R Hunter
Journal: Blood (2021)
DNMT3A Mutations Should be Considered in the Risk Stratification for Pediatric and Adolescent and Young Adult Patients with Acute Myeloid Leukemia
Authors: Dristhi Ragoonanan, Avis Harden, Amr Elgehiny, Cesar Nunez, Michael E. Roth, David McCall, Branko Cuglievan, Priti Tewari, Demetrios Petropoulos, Kris M Mahadeo, Sajad J Khazal
Journal: Blood (2021)
Prognostic Impact of CD3 Count in Apheresis Collection in Multiple Myeloma Patients Undergoing Autologous Stem Cell Transplant
Authors: Marcella Kaddoura, Morie A. Gertz, David Dingli, Francis K. Buadi, Martha Q. Lacy, Angela Dispenzieri, Prashant Kapoor, Suzanne R. Hayman, Wilson I Gonsalves, Taxiarchis Kourelis, Rahma M Warsame, Eapen K. Jacob, Shaji Kumar
Journal: Blood (2021)
NHS is facing most difficult winter in its history, trust leaders warn
Authors: Gareth Iacobucci
Journal: BMJ (2021)

NHS hospitals are already “beyond full stretch” and are preparing for the most difficult winter in the health service’s history, trust leaders have warned.

NHS Providers, which represents all hospitals, mental health trusts, and community and ambulance services in England, said the combined impact of high demand for emergency care, growing waiting lists, chronic staff shortages, potential staff burnout, vaccination campaigns, and the prospect of high levels of covid-19, flu, and other respiratory viruses was causing huge anxiety among trust leaders.

The organisation’s annual survey of trust leaders,1 launched on the first day of its …
Public health measures for covid-19
Authors: Paul P Glasziou, Susan Michie, Atle Fretheim
Journal: BMJ (2021)

Somatic hypermutation (SHM) of Ig genes is the result of a two-phase process initiated by activation-induced cytidine deaminase, relying on two different strategies for the introduction of mutations at CG pairs (phase I) and at AT pairs (phase II). To explain the selectivity of phase II, two mechanisms were proposed: AT-selective error-prone DNA-polymerases, deoxyuridine triphosphate (dUTP) incorporation, or both. However, there has been no experimental evidence so far of the possible involvement of the latter. We have developed a ligation-anchored PCR method based on the formation of single-strand breaks at uracils. In this study, we show the presence of uracil in hypermutating <i>VkOx</i> genes in wild type (AID<sup>+/+</sup>) mice, demonstrating that dUTP incorporation via DNA polymerases could be a major mechanism in SHM. Thus, error-prone DNA polymerases would participate in SHM via low-fidelity replication and incorporation of dUTP.
Abstract 9931: Baseline Characteristics of Patients with Peripartum Cardiomyopathy; Mortality versus Left Ventricular Ejection Fraction Recovery Groups
Authors: Hasan Ali Farhan, Israa Fadhil Yaseen
Journal: Circulation (2021)

Introduction: Data about long term outcomes among patients with peripartum cardiomyopathy (PPCM) is limited. According to the 2018 ESC guideline for the management of cardiovascular disease during pregnancy, three factors are associated with adverse outcomes including baseline LVEF &lt; 30%, LVEDD ≥ 60 mm, and right ventricular involvement. Purpose: To identify the baseline characteristics between two groups of patients with PPCM; patients with LVEF recovery within first year after diagnosis and patients who died within the same period after diagnosis. Also to identify the specific outcomes of the two groups. Methods: Retrospectively data were analyzed including patients with PPCM who had LVEF recovery within first year of diagnosis versus patients who died within one year after diagnosis. Data collected at cardio-obstetric clinic between January 2015 - November 2020. Results: Total number of patients who died within the first years were six patients while the total number of patients who had LVEF recovery within 1 year was 14 patients. Their clinical characteristics and outcomes are shown in table 1. Conclusions: Mortality group had less hypertension co-morbid disease, lower baseline systolic blood pressure, higher heart rate, lower LVEF, more right ventricular dysfunction, and less to be diagnosed in the postpartum period compared with LVEF recovered group. Use of spironolactone and bromocriptine were limited among both groups and light should be focus on this gap to be bridged. Lower rate of ACE-inhibitor use among mortality group may be explained by lower systolic blood pressure in this cohort. Most of the patients had LVEF recovery within the first 6-month and most of the patients in the other group died within the same period with sudden cardiac death as the leading cause of mortality.
Abstract 14189: Isolated Tricuspid Valve Surgery Repair versus Replacement for Infective Endocarditis: A Nationwide Analysis
Authors: Agam Bansal, Mohamed Gad, Paul Cremer, Wael A Jaber, Venu Menon, heba wassif
Journal: Circulation (2021)

Introduction: Incidence of right-sided infective endocarditis (IE) is on rise secondary to opioid epidemic, and increasing use of cardiac devices, central catheters, or AV fistulas. We aimed to compare outcomes of isolated tricuspid valve (TV) repair versus replacement and determine the predictors of mortality after isolated TV surgery for hospitalizations with IE. Methods: We utilized nationwide readmission database (NRD) to identify hospitalizations for IE that underwent isolated TV repair or replacement in 2018. Hospitalizations with concomitant or previous cardiac surgery were excluded. Multivariate logistic regression analysis was performed to determine predictors of mortality after isolated TV surgery for IE. Results: 1463 hospitalizations were identified for IE undergoing either isolated TV repair (n=531, 36.3%) or replacement (n=932, 63.7%). 106 (11.3%) underwent mechanical TV replacement. Patients undergoing TV replacement had increased risk of acute kidney injury (aOR: 1.45 (1.03-2.03), p=0.03) and pacemaker implantation (aOR: 7.03 (3.95-13.45), p&lt;0.001), however there were no significant differences in in-hospital mortality (aOR: 0.80 (0.35-1.83), p=0.59) (Table). Independent predictors of mortality for TV replacement were advanced liver disease (ALD) (5.31 (1.62-17.67), p=0.005), and acute heart failure (3.48 (1.02-18.14), p=0.044) and for TV repair were ALD (5.72 (1.31-24.90), p=0.017), and age (1.07 (1.02-1.14), p=0.026). However, predisposing conditions (drug use, infection of cardiac prosthesis, or vascular device), micro-organisms, and mechanical TV replacement were not associated with mortality in patients undergoing surgery. Conclusions: Our study reinforces that TV repair should be preferred over replacement for right sided IE when feasible, and adds to existing literature that it is the patient’s co-morbidities rather than the predisposing factors or microbiology that is associated with increased mortality.
Slit3 secreted from M2-like macrophages increases sympathetic activity and thermogenesis in adipose tissue
Authors: Yi-Na Wang, Yan Tang, Zhihui He, Hong Ma, Linyuan Wang, Yang Liu, Qiqi Yang, Dongning Pan, Cuiqing Zhu, Shuwen Qian, Qi-Qun Tang
Journal: Nature Metabolism (2021)
Protective efficacy of 6-week regimen for latent tuberculosis infection treatment in rural China: 5-year follow-up of a randomised controlled trial
Authors: Henan Xin, Xuefang Cao, Haoran Zhang, Boxuan Feng, Ying Du, Bin Zhang, Dakuan Wang, Zisen Liu, Ling Guan, Fei Shen, Xueling Guan, Jiaoxia Yan, Yijun He, Yongpeng He, Zhusheng Quan, Shouguo Pan, Jianmin Liu, Qi Jin, Lei Gao
Journal: European Respiratory Journal (2021)

Enlarging tuberculosis (TB) preventive treatment among at-risk populations is a critical component of the End TB Strategy. There is an urgent need to develop suitable latent tuberculosis infection (LTBI) testing and treatment tools according to the local TB epidemic and available resources worldwide.Based on an open-label randomised controlled trial conducted since 2015 in China among rural residents aged 50-70 years with LTBI, the protective efficacy of a 6-week twice-weekly regimen of rifapentine plus isoniazid was further evaluated in a 5-year follow-up survey.1298 treated participants and 1151 untreated controls were included in the 5-year protective efficacy analysis. In the per-protocol analysis, the incidence rate was 0.49 (95% CI 0.30-0.67) per 100 person-years in the untreated control group and 0.19 (95% CI 0.07-0.32) per 100 person-years in the treated group; the protection rate was 61.22%. Subgroup analysis showed that the protection rate was 76.82% in the per-protocol analysis among participants with baseline interferon (IFN)-γ levels in the highest quartile (≥3.25 IU·mL-1). Multiple logistic regression analysis indicated that participants with baseline body mass index <18.5 kg·m-2 and with pulmonary fibrotic lesions had increased hazard of developing active disease with an adjusted hazard ratio (aHR) of 3.64 (95% CI 1.20-11.00) and 5.99 (95% CI 2.20-16.27), respectively. In addition, individuals with higher baseline IFN-γ levels showed an increased risk of TB occurrence (aHR 2.27, 95% CI 1.13-4.58).Our findings suggest the 6-week twice-weekly regimen of rifapentine plus isoniazid for LTBI treatment might be an optional tool for TB control in the Chinese population.
The CAR-Hematotox Identifies Patients at High Risk for Prolonged Neutropenia, Infectious Complications and Prolonged Hospitalization Following CD19-CART in R/R LBCL
Authors: Kai Rejeski, Ariel Perez-Perez, Gloria Iacoboni, Olaf Penack, Veit L Buecklein, Liv Jentzsch, Dimitrios Mougiakakos, Grace Johnson, Brian Arciola, Viktoria Blumenberg, Cecilia Carpio, Eva Hoster, Lars Bullinger, Frederick L. Locke, Michael von Bergwelt, Andreas Mackensen, Wolfgang A Bethge, Pere Barba, Michael D. Jain, Marion Subklewe
Journal: Blood (2021)
From health to climate there is disrespect at the heart of every crisis
Authors: Kamran Abbasi
Journal: BMJ (2021)

Is the prime minister a king? The outrage at Boris Johnson appearing without a mask in so many photos in the media, from sitting beside David Attenborough at the COP26 climate conference to touring Hexham General Hospital in Northumberland, shows that the court of public opinion will let you get away with only so much (https://www.independent.co.uk/news/health/boris-johnson-mask-nhs-hospital-backlash-b1954209.html; doi:10.1136/bmj.n2682).12

Ironically, healthcare management and leadership are currently high profile areas of government scrutiny, but the premise and objectives behind the scrutiny are misguided, argues Nigel Edwards (doi:10.1136/bmj.n2618).3 “The scale, complexity, and political nature of the health service,” he explains, “limit the applicability of ideas and interventions from elsewhere.” Parachuting in leaders from other industries hasn’t worked before in …
Mobilized Mouse and Human Peripheral Blood Containing Elevated Numbers of Donor Treg Cells Ameliorates Pre-Clinical GvHD and GVL Is Maintained in an MHC-Matched Allogeneic Murine Model
Authors: Henry Barreras, Sabrina N. Copsel, Ying Ding, Charles J. Cash, Cameron S. Bader, Cara L. Benjamin, Krishna V. Komanduri, Thomas R. Malek, Robert B. Levy
Journal: Blood (2021)
Primary Clinical Response of Relmacabtagene Autoleucel in Adults with Relapsed/Refractory Follicular Lymphoma (r/r FL) in China
Authors: Yuqin Song, Zhitao Ying, Haiyan Yang, Ye Guo, Wenyu Li, Dehui Zou, Jianqiu Wu, Su Yang, Pian Zhang, Meilin Ma, Zisong Zhou, Hongxia Zheng, Jun Zhu
Journal: Blood (2021)