tbuckley/cabot-search huggingface dataset:
Frog cardiac calsequestrin. Identification, characterization, and subcellular distribution in two structurally distinct regions of peripheral sarcoplasmic reticulum in frog ventricular myocardium.
Authors: A GMcLeod, A CShen, K PCampbell, MMichalak, A OJorgensen
Journal: Circulation Research (1991)

Calsequestrin is a calcium-binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum (SR) of muscle cells during relaxation. In the present study, we used affinity-purified antibodies to chicken cardiac calsequestrin to identify a 60,000-Da calsequestrin in frog myocardium. Like previously identified cardiac calsequestrins, it is enriched in cardiac microsomes, it is enriched by biochemical procedures previously used to purify cardiac and skeletal calsequestrins, and it exhibits a pH-dependent shift in its apparent Mr on a two-dimensional gel system. Finally, the NH2-terminal amino acid sequence of this 60,000-Da immunoreactive protein purified by fast protein liquid chromatography was identical to that of rabbit skeletal and canine cardiac calsequestrin. Thus, we conclude that this protein corresponds to the calsequestrin isoform in frog ventricular muscle. Frog calsequestrin was localized in discrete foci present at the periphery but absent from the central regions of frog ven...
Interviews with patients better than surveys for generating change
Authors: Chris Mahony
Journal: BMJ (2003)

Intensive interviews with patients and carers generate far more valuable ideas for service change than traditional patient satisfaction surveys, the Coronary Heart Disease Collaborative has claimed.

The collaborative, which has developed its own model for interviews, says that as well as providing ideas for improving services, reading transcripts or playing tapes of the interviews can have a powerful impact on clinical staff.

Dr Mark Dancy, consultant cardiologist at North West London Hospitals NHS Trust and clinical chair of the collaborative, outlined the work at a conference on redesigning NHS service delivery held by the Health Service Journal in London last week.

Dr Dancy said: “The interviews are aimed at finding out what it is like to be a person with a particular condition or to care for someone with that condition. Healthcare professionals sometimes find it difficult to put themselves in the shoes of patients.”

He suggested that as well as giving more detailed insight into the patient experience, semistructured interviews probably provided a more reliable guide to service quality than patient satisfaction surveys.
Activation-dependent cumulative depletions of extracellular free calcium in guinea pig atrium measured with antipyrylazo III and tetramethylmurexide.
Authors: D WHilgemann, M JDelay, G ALanger
Journal: Circulation Research (1983)

We have used a spectrophotometric method to monitor mean free extracellular calcium concentrations in isolated left atria of guinea pigs via extracellular application of the calcium-sensitive absorption dyes, antipyrylazo III and tetramethylmurexide. Exchange of extracellular free calcium with the bathing medium takes several minutes and closely parallels contractile response in this preparation. Under conditions favoring a rapid positive force staircase response during repetitive stimulation after a long rest period (2-10 minutes), cumulative depletions of extracellular calcium can clearly be differentiated from motion artifact due to muscle movement by multiple-wavelength spectrophotometry. Responses of similar magnitude and characteristics are obtained with both dyes employed. In the presence of 10(-7) M isoproterenol, the mean extracellular calcium concentration falls by at least 5% (0.25-0.8 mM total calcium concentration) in four beats at 0.5 Hz; extracellular calcium replenishes during rest with an...
Radioimmunoassay for human myoglobin. Initial experience in patients with coronary heart disease.
Authors: MReichlin, J PVisco, F JKlocke
Journal: Circulation (1978)

A radioimmunoassay for human myoglobin has been used to study the serum myoglobin level in 13 normal individuals and 68 patients admitted to a Coronary Care Unit because of chest pain. Values in normal individuals ranged from 3 to 75 and averaged 25 +/- 23 (SD) ng/ml. Thirty-two patients with myocardial infarction initially examined within 12 hours of the onset of chest pain all showed clear-cut elevations in serum myoglobin, peak values ranging from 200 to 5500 and averaging 1368 +/- 1357 ng/ml. Seventeen patients with clinically atypical chest pain and no subsequent evidence of myocardial necrosis had myoglobin levels in the normal range, as did 11 of 19 patients with chest pain thought clinically to represent myocardial ischemia but no subsequent evidence of myocardial necrosis by conventional criteria. The final eight patients in the latter group showed mild elevations of serum Mb, peak values ranging from 102 to 280 and averaging 162 +/- 52 ng/ml; the basis for these elevations remains to be clarified.
Relationship of the lytic state to successful reperfusion with standard- and low-dose intracoronary streptokinase.
Authors: R LRothbard, P GFitzpatrick, C WFrancis, D MCaton, W BHood, V JMarder
Journal: Circulation (1985)

The influence of a systemic lytic state on reperfusion obtained after intracoronary streptokinase (SK) therapy has been evaluated in 15 patients with acute myocardial infarction and complete coronary occlusion. Coronary angiographic studies and measurements of blood fibrinolytic parameters were repeated at 15 min intervals during the infusion of a standard dose of SK and were compared with the results with approximately one-tenth the standard dose. Successful reperfusion was obtained in only 20% (2/10) of patients receiving the low dose, compared with a 75% to 80% success rate in patients receiving the standard dose as initial treatment (4/5) or as follow-up treatment of patients in whom low-dose therapy failed (6/8). There was a striking association between reperfusion and development of the lytic state in that all 12 treatments resulting in reperfusion also caused a lytic state and all seven treatments that failed to produce a lytic state also failed to induce reperfusion (p less than .001). Among the s...
TCTAP A-047 Are Diabetic Patients with Above 0.8 FFR Really Safe?
Authors: Kyu-Sun Lee, Bon-Kwon Koo, Seokhun Yang, Doyeon Hwang, Gilwoo Choi, Joo Myung Lee, Eun-Seok Shin, Joon-Hyung Doh, Chang-Wook Nam, Hyuk-Jae Chang, Kyung Woo Park, Hyun-Jae Kang, Hyo-Soo Kim
Journal: Journal of the American College of Cardiology (2021)
A comparison of simultaneous measurements of systolic function in the baboon by electromagnetic flowmeter and high frame rate ECG-gated blood pool scintigraphy.
Authors: M VGreen, H GOstrow, R NScott, M ADouglas, J JBailey, G SJohnston
Journal: Circulation (1979)

Left ventricular (LV) systolic timing and relative volume variations were simultaneously measured by electromagnetic flowmeter (EMF) and high frame rate ECG-gated blood pool scintigraphy in five baboons. No significant differences (p greater than 0.1, paired t test) were observed in the time (from R wave) to peak aortic flow (maximum LV ejection rate), time to cessation of aortic flow (end-systole) or in the duration of aortic flow (LV ejection time). A small (approximately 15 msec) but significant systematic difference (p less than 0.02) was noted in the time to onset of aortic flow. The shape of each scintigraphic time-activity curve during systole was compared to an equivalent curve synthesized from 10 EMF flow profiles obtained in the same baboon. Comparison of these paired curves over systolic ejection yielded an average correlation or r = 0.95 (range 0.90--0.99). The ratio of peak flow to stroke volume determined from these data did not differ significantly (p greater than 0.05). In the baboon, quan...
Ileoscopy Yield During Colonoscopy Procedures: An Indication-Based Analysis
Authors: F Abu Baker, T Khoury, M Mahamid, A Shorbaji, Y Kopelman, A Mari
Journal: Endoscopy (2021)

Aims While the routine performance of ileoscopy during colonoscopy procedures is perceived to have a low yield, its utility during colonoscopies performed for specific indications have not been well studied. The current study aims to assess the diagnostic yield of indication-based ileoscopies in the real life practice.
Anatomic correlates of aortic pulse wave velocity and carotid artery elasticity during atherosclerosis progression and regression in monkeys.
Authors: D JFarrar, M GBond, W ARiley, J KSawyer
Journal: Circulation (1991)

We noninvasively measured changes in average aortic stiffness in 79 cynomolgus monkeys being fed cholesterol progression, regression, and control diets by measuring pulse wave velocity (PWV) in 260 experiments during a 30-month period. Every 6 months, a group of monkeys was studied with invasive aortic PWV techniques and with ultrasonically determined pressure-strain elastic modulus (Ep) of the carotid artery, and then the group was killed so that morphometric evaluation of atherosclerosis severity could be made. After 6 months of a cholesterol progression diet, PWV decreased slightly from 6.2 +/- 0.1 to 5.7 +/- 0.1 m/sec, followed by an approximate linear increase to 8.8 +/- 1.2 m/sec after 30 months on the diet. The corresponding ratio of intimal (plaque) area to medial area (IA/MA) measured on perfusion-fixed cross-sections of the abdominal and thoracic aortas increased from 0.16 +/- 0.07 at 6 months to 1.23 +/- 0.22 at 30 months. Monkeys in the regression groups were fed the cholesterol progression di...
Unilateral Renal Vein Thrombosis with Nephrotic Syndrome
Authors: R. C. Khokhani, P. Raghavan, S. M. Sant, R. D. Ganatra, S. M. Sharma, M. J. Shah
Journal: BMJ (1966)

MEDICURNAL 1523 attacks, occurring between 8.30 and 9 a.m., were noted to follow runs of coupled ectopic beats, and recurrent ventricular fibrillation was shown by E.C.G. (Fig. 1).Each attack either terminated spontaneously or was terminated by external cardiac massage, but the basic dysrhythmia of coupled ventricular ectopic beats persisted.8 ..... .. ... .... 4.. if-fi .t. f Z. ~~~~~~... FIG.2.-Electrocardiogram taken before and after intravenous administra tion of propranolol.
Predicting an accelerated lung function decline in smokers: is there a proper threshold?
Authors: Firdaus A.A. Mohamed Hoesein, Pieter Zanen
Journal: European Respiratory Journal (2013)

Predicting an accelerated lung function decline in smokers: is there a proper threshold?To the Editor:We read, with very much interest, the publication by AKKERMANS et al. [1] in the European Respiratory Journal.In this study they reanalysed data from the first Lung Health Study by forming four groups, based on the forced expiratory volume in 1 s (FEV1)/forced vital capacity (FVC) ,0.70 or ,5th percentile, according to the LMS (lambda, mu, sigma) approach.However, we do have some remarks concerning the validity of their analysis and propose a means of reinterpretation of their outcome. AKKERMANS et al.[1] concluded that the decline of the post bronchodilator FEV1 in the group fixed + /LMS - (FEV1/FVC ,0.70 and .5thpercentile) was significantly lower compared to the group fixed + /LMS + (FEV1/ FVC ,0.70 and ,5th percentile).The mean¡SD decline was 43.8¡50.0mL?year -1 versus 53.5¡51.5 mL?year -1 , respectively, and thus one recommended the use of the 5th percentile threshold to define the presence of chronic obstructive pulmonary disease (COPD).Interestingly, this conclusion was reached after they excluded, in total, 1842 subjects from the initial 5887 subjects.Therefore, the question arises: what is the consequence of such a vast post hoc exclusion on the estimates of the FEV1 decline in the two groups?The majority of subjects (n51276 ) were excluded as they showed ''an unstable classification'' and one can ask oneself what the FEV1 decline was in those excluded subjects.Some were reported to have an improved lung function over time and some were reported to have become more obstructive.Data, on whether this exclusion was ''randomised'' over the four groups formed, is lacking.The text of the publication does not give detailed information on the reasons as to why the authors chose to follow this path.Anyway, one must realise that it is highly likely that the groups, thus formed, suffer from selection bias and the outcome of the analysis is, therefore, also subject to some degree of bias.This selection bias is illustrated in table 1 of the article [1] where it is shown that the excluded subjects had a better FEV1 and FEV1/FVC, despite the same amounts of pack-years.
Electrotonic metabolism of pacemaker activity. Further biological and mathematical observations on the behavior of modulated parasystole.
Authors: CAntzelevitch, JJalife, G KMoe
Journal: Circulation (1982)

An in vitro biologic model of parasystole and a mathematical model of parasystole based on the phase-response relationships derived from the biologic model were used in tandem to further develop our understanding of the patterns of  activity that might arise as a consequence of the interaction of two pacemakers across a zone of block. Superfusion of the central segment of a dog Purkinje fiber with an ion-free isotonic sucrose solution provided a narrow region of block. The modulation of pacemaker activity by electronic potentials transmitted across the area of block was shown to be importantly influenced by the position of the ectopic pacemaker relative to the site of block. Effects of repetitive electrotonic influences on a single pacemaker cycle, the degree of entrance block and capture of the pacemaker during a phase of supernormal excitability were also studied in both the biologic and mathematical models. Our results indicate that marked shifts in the incidence and pattern of manifest ectopi...
Effects of external pH on ionic currents in smooth muscle cells from the basilar artery of the guinea pig.
Authors: G AWest, D CLeppla, J MSimard
Journal: Circulation Research (1992)

pHo is an important determinant of vascular tone in cerebral blood vessels. We investigated the effects of changes in pHo on isolated smooth muscle cells from the basilar artery of the guinea pig. Single cells contracted rapidly in response to an elevation in pHo (constant CO2), and contraction was blocked by nifedipine, suggesting a role for dihydropyridine-sensitive Ca2+ channels. In whole-cell patch-clamp experiments, changes in pHo (pHo 5.7-8.1, pHi 7.2 with 10 mM HEPES) strongly affected the amplitude of the peak Ca2+ channel current (10 mM Ba2+, +15 mV, holding potential of -55 mV), with an apparent pK of 6.9. The current-voltage curves were minimally shifted, indicating no important effect of surface charge. To separate the slowly inactivating L-type Ca2+ channel current from the more rapidly inactivating B-type current, the decaying portions of inward currents from cells studied with repetitive 1-second pulses (+15 mV, holding potential of -55 mV) were fit to a two-component model. Titration curve...
Regional oxidative metabolism in patients after recovery from reperfused anterior myocardial infarction. Relation to regional blood flow and glucose uptake.
Authors: J LVanoverschelde, J AMelin, ABol, RVanbutsele, MCogneau, DLabar, ARobert, CMichel, WWijns
Journal: Circulation (1992)

BACKGROUNDEnhanced uptake of the glucose analogue 18F-fluorodeoxyglucose (FDG) in relation to flow has been proposed as an accurate method of identifying viable myocardium. The evaluation of myocardial oxidative metabolism could be an alternate way to identify reversible injury. The aim of the present study was to investigate in patients with reperfused anterior infarction whether differences in regional oxidative metabolism exist among regions with and without flow-metabolism mismatch.METHODS AND RESULTSFifteen patients with reperfused anterior myocardial infarction were studied between 2 weeks and 3 months after the acute event. Regional myocardial blood flow (13N-ammonia; three-compartment model), oxidative metabolism (11C-acetate; monoexponential clearance), and glucose uptake (FDG, linear graphic analysis) were evaluated with dynamic positron emission tomography. Flow-metabolism patterns were used to differentiate reversibly (FDG/flow greater than 1.2) from irreversibly injured myocardium (FDG/flow l...
Atrial natriuretic factor-induced systemic vasoconstriction in conscious dogs, rats, and monkeys.
Authors: Y TShen, M AYoung, JOhanian, R MGraham, S FVatner
Journal: Circulation Research (1990)

This study addresses the hypothesis that atrial natriuretic factor (ANF) is a primary vasodilator, which reduces arterial pressure directly and increases total peripheral resistance secondarily by reflex mechanisms. The effects of 30-minute infusions of ANF (0.3 micrograms/kg/min i.v.) were examined in conscious dogs, rats, and monkeys before and after ganglionic blockade with hexamethonium. In seven intact, conscious dogs, ANF reduced mean arterial pressure by 7 +/- 1% and cardiac output by 19 +/- 3% and increased total peripheral resistance by 15 +/- 3%. After ganglionic blockade, ANF reduced mean arterial pressure by 7 +/- 2% but still increased total peripheral resistance by 15 +/- 3%. Similar results were observed in four dogs with total cardiac denervation and in six dogs with arterial baroreceptor denervation. Furthermore, in two dogs, combined ganglionic and alpha 1-adrenoceptor blockades failed to alter the rise in total peripheral resistance observed with ANF. In six intact, conscious rats, ANF ...
Long-term beta-blockade in dilated cardiomyopathy. Effects of short- and long-term metoprolol treatment followed by withdrawal and readministration of metoprolol.
Authors: FWaagstein, KCaidahl, IWallentin, C HBergh, AHjalmarson
Journal: Circulation (1989)

To evaluate the short- and long-term effects of beta-adrenergic blockade (metoprolol) as well as the reaction to withdrawal and readministration of metoprolol in severe heart failure, 33 patients (25 men and eight women; mean age, 47.6 +/- 14.0 years) with dilated cardiomyopathy were studied by right and left heart catheterization, right ventricular biopsy, two-dimensional and Doppler echocardiography, and external pulse recordings. Twenty-six of 33 patients survived more than 6 months, and 24 of the 26 patients improved their functional class (from mean 3.3 to 1.8, p less than 0.0001). These 24 patients were subjected to withdrawal of metoprolol until the number of symptoms increased and deterioration occurred as observed noninvasively (group 1, n = 16), whereas the eight patients did not deteriorate during a 12-month period (group 2). During long-term treatment with metoprolol, there was an increase in ejection fraction from 0.24 to 0.42 (p less than 0.0001), whereas there was a decrease in the left ven...
Unusual examples of supraventricular re-entrant tachycardias.
Authors: H JWellens
Journal: Circulation (1975)

Programmed electrical stimulation of the heart uncovered unusual findings in four patients suffering from supraventricular tachycardias. They included 1) two supraventricular tachycardias of different location and frequency in the same patient, 2) increase in rate of a re-entrant atrioventricular (A-V) nodal tachycardia followed by the development of 2-to-1 A-V block, 3) a gap in A-V conduction and A-V nodal re-entrant tachycardia with 2-to-1 A-V block, and 4) the initiation of atrial re-entrant tachycardia by ventricular premature beats. The examples are given to help elucidate the understanding of mechanisms involved in re-entrant tachycardias and to localize the site of the re-entry circuit.
Different Biomarker Modalities and Response to Anti–PD-1/PD-L1 Therapies
Authors: Shufang Liu, Wenquan Niu
Journal: JAMA Oncology (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Oncology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Oncology journal
Two-dimensional echocardiographic examination of mitral valve abnormalities associated with coarctation of the aorta.
Authors: VCelano, D RPieroni, J AMorera, J MRoland, R LGingell
Journal: Circulation (1984)

Coarctation of the aorta is frequently associated with left ventricular inflow tract abnormalities that may be difficult to detect even at cardiac catheterization. This study involved patients with coarctation who underwent comprehensive two-dimensional echocardiographic investigations emphasizing visualization of the mitral valve and its apparatus. Of the 56 patients studied, 23 had completely normal study results, while in 33 (59%) results showed abnormalities of the mitral complex. The latter were divided into two groups: those having major mitral abnormalities (n = 12) and those with minor anomalies of the valve and apparatus (n = 21). The first group included patients with a supravalve stenosing ring, congenital mitral stenosis, mitral valve prolapse, and parachute mitral valve. Minor anomalies were classified as abnormalities of the papillary muscles, chordae tendineae, or combinations of both. No mitral malformations were found in a control group of patients. We conclude that two-dimensional echoca...
<i>VPS4A</i> : A Novel Candidate Gene for Congenital Dyserythropoietic Anemia
Authors: Katie Giger, Georgios E Christakopoulos, Lisa Trump, Clarissa E. Johnson, Haripriya Sakthivel, Mary Risinger, Omar Niss, Kejian Zhang, Lionel Blanc, Robert B. Lorsbach, Paritha I. Arumugam, Carolyn Lutzko, Theodosia A. Kalfa
Journal: Blood (2017)
216P COMMD1 in non-small cell lung cancer: A novel DNA repair protein as a therapeutic target and diagnostic biomarker
Authors: A. Suraweera, P. Duijf, M. Tang, C. Jekimovs, K. Schrobback, C. Liu, M. Adams, D. Richard, K. O'Byrne
Journal: Journal of Thoracic Oncology (2021)