tbuckley/cabot-search huggingface dataset:
Abstract P3-04-01: Telomere G-tail length correlates with the presence of breast cancer
Authors: Yumiko Koi, Yasuhiro Tsutani, Yukie Nishiyama, Shinsuke Sasada, Norio Masumoto, Takayuki Kadoya, Ryou-u Takahashi, Morihito Okada, Hidetoshi Tahara
Journal: Cancer Research (2020)

Abstract Background The telomere G-tail, located at the terminal 3’-end, plays an important role in the maintenance of chromosome stability. Telomere G-tail length has been shown to be related to the presence of various cancers; however, the association between telomere G-tail length and breast cancer has remained unclear. Methods We measured the length of the leukocyte telomere G-tail using the hybridization protection assay, which has the advantages of being simple to perform, accurate and highly sensitive for G-tails as short as 20 nucleotides. The association between telomere G-tail length and presence of breast cancer was estimated by comparing breast cancer patients (n=70) and cancer-free individuals (n=83). We recruited cStage I-III breast cancer patients who underwent surgery between November 2016 and December 2017 at Hiroshima University Hospital. Cancer-free individuals included age-matched female individuals with no previous or current history of any cancer. Results The median telomere G-tail length was significantly shorter in breast cancer patients (11,886.70 ± 2,008.74 RLU/μg DNA) than in cancer-free individuals (13,336.00 ± 2,072.31 RLU/μg DNA; p &amp;lt; 0.01). Shortening of the telomere G-tail length was observed in the phase of ductal carcinoma in situ, and this trend was observed with or without shortening of the total telomere length. Spearman’s rank correlation analysis showed that there was no significant association between telomere G-tail length and total telomere length in breast cancer patients (ρ = -0.09; p = 0.48). In contrast, a significant correlation was found between telomere G-tail length and total telomere length in cancer-free individuals (ρ = 0.29; p = 0.01). Conclusions Our study indicated that leukocyte telomere G-tail length may serve as a novel potential biomarker for detection of breast cancer. Citation Format: Yumiko Koi, Yasuhiro Tsutani, Yukie Nishiyama, Shinsuke Sasada, Norio Masumoto, Takayuki Kadoya, Ryou-u Takahashi, Morihito Okada, Hidetoshi Tahara. Telomere G-tail length correlates with the presence of breast cancer [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P3-04-01.
Abstract P5-08-22: Associations between MYC alterations and clinical pathological characteristics in Chinese patients and TCGA cohort
Authors: Li Cao, Guochun Zhang, Yulei Wang, Bo Chen, Chongyang Ren, Lingzhu Wen, Liping Guo, Kai Li, Minghan Jia, Ning Liao
Journal: Cancer Research (2020)

Abstract Background MYC is frequently amplified and its amplification is associated with poor prognosis in breast cancer patients. This study aimed to determine the prevalence of MYC alterations from Chinese patients, the correlations between MYC amplification and clinicopathological characteristics, as well as its impacts on patient outcomes in breast cancer patients. Methods We analyzed 412 cases of breast cancer specimens in Guangdong Provincial People's Hospital (GDPH) for MYC alterations from June 1, 2017 to September 27, 2018, using next generation sequencing. The associations between MYC amplification and clinicopathological characteristics were determined using chi square. We further compared the our results with those from The Cancer Genome Atlas (TCGA) cohort (n=1079). Kaplan-Meier curves were drawn to determine the impacts of MYC amplification on patient outcomes. Results MYC was amplified in 12.44% (51/410) in GDPH cohort and the mean copy number of our cohort was 4.42 (2.84-11.27), while the frequency of MYC amplification in TCGA cohort was much higher (21.22%, 229/1079). Except that, we also found two fusions. Among these, one was RTFDC1-MYC fusion (AF=1.62%) and the other was PVT1-MYC fusion (AF=3.33%). The case with PVT1-MYC fusion was also found to have PVT1-MYC co-amplification concurrently. In TCGA cohort, we found MYC amplification to be associated with age (P=0.047), tumor type (P=0.000), ER status (P=0.000), PR status (P=0.000), HER2 status (P=0.008) and molecular subtype (P=0.000). Whereas, except for molecular subtype (P=0.028), we only found MYC amplification to be associated with tumor size (P=0.023) and Ki-67 (P=0.031) in GDPH cohort. According to the survival information from TCGA, patients with MYC amplification had significantly inferior overall survival (OS) in both triple negative breast cancer (TNBC) (P=0.02) and HR+/HER2+ breast cancer patients (P=0.03). Conclusions This study evaluated the differences of MYC mutations in Chinese and and TCGA cohort breast cancer patients. Except for MYC amplification, we found two rare fusions. Results showed MYC amplified less frequently in Chinese patients and was associated with inferior OS in both TNBC and HR+/HER2+ breast cancer patients. We suggest further research with expand the sample size is necessary to draw the ethnical differences of MYC mutations. Citation Format: Li Cao, Guochun Zhang, Yulei Wang, Bo Chen, Chongyang Ren, Lingzhu Wen, Liping Guo, Kai Li, Minghan Jia, Ning Liao. Associations between MYC alterations and clinical pathological characteristics in Chinese patients and TCGA cohort [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P5-08-22.
Randomized Trials in Cardiac Surgery
Authors: Mario Gaudino, A. Pieter Kappetein, Antonino Di Franco, Emilia Bagiella, Deepak L. Bhatt, Andreas Boening, Mary E. Charlson, Marcus Flather, Annetine C. Gelijns, Frederick Grover, Stuart J. Head, Peter Jüni, Andre Lamy, Marissa Miller, Alan Moskowitz, Wilko Reents, A. Laurie Shroyer, David P. Taggart, Derrick Y. Tam, Marco A. Zenati, Stephen E. Fremes
Journal: Journal of the American College of Cardiology (2020)
2006-2007 年度合同研究班報告.冠攣縮性狭心症の診断と治療に関するガイドライン
Authors: 小川久雄, 隆史 赤阪, 謙 奥村, 成乃亮 川嶋, 道雄 川筋, 一雄 木村, 穎 斎藤, 宏明 下川, 章三 末田, 陽一 嶽山, 恭彦 田辺, 和文 土橋, 孝一 野出, 隆一 服部, 杏一 水野, 邦久 三羽, 豊明 室原, 正博 毛利, 正和 山岸, 道博 吉村, 晃男 井上, 周生 雪吹, 晃 大下, 幸一 海北, 宏明 河野, 淳 小島, 雅美 小菅, 弘文 副島, 滋穂 財田, 雅文 中山, 聡 安田, 浩 岸田, 仁暢 友池, 一夫 土師, 光宏 横山
Journal: Circulation (2008)
A Dynamic Immune Response Shapes COVID-19 Progression
Authors: Eugenia Ziying Ong, Yvonne Fu Zi Chan, Wan Ying Leong, Natalie Mei Ying Lee, Shirin Kalimuddin, Salahudeen Mohamed Haja Mohideen, Kian Sing Chan, Anthony Tanoto Tan, Antonio Bertoletti, Eng Eong Ooi, Jenny Guek Hong Low
Journal: Cell Host & Microbe (2020)
Genotyping and Subtyping <i>Cryptosporidium</i> To Identify Risk Factors and Transmission Patterns — Nebraska, 2015–2017
Authors: Brianna K. Loeck, Caitlin Pedati, Peter C. Iwen, Emily McCutchen, Dawn M. Roellig, Michele C. Hlavsa, Kathleen Fullerton, Thomas Safranek, Anna V. Carlson
Journal: MMWR Morbidity and Mortality Weekly Report (2020)

Molecular typing of Cryptosporidium in Nebraska during 2015â2017 found that C. parvum cases were associated with animal exposures in rural settings, whereas C. hominis cases were more likely to occur in urban areas.
Abstract P3-01-21: Functional duality of NUAK2 in breast cancer metastases
Authors: Wang Jia, XUE Jingyan, Hou Jianjing, Xiu Bingqiu, Li Lun, Chi Yayun, Wu Jiong
Journal: Cancer Research (2020)

Abstract Background: Breast cancer patients with lymph nodes metastasis were proven to have more aggressive biologically phenotypes. This study was aimed to find different biomarker ectopic expression between breast cancer patients' metastatic lymph nodes and primary cancer, and to explore the potential molecular mechanisms. Method: From our previous research, we selected the pairing samples from primary breast cancer with their early stage lymph node metastasis to carry out differential gene expression profiling. Genes with high expression level in sentinel lymph node metastasis were obtained. Then according to the findings of CRISPR/Cas9 screening and the results in relevant literature and online public database, we decided to investigate the role of NUAK2 in our further research. In vitro and in vivo analyses were carried out to determine the potential mechanisms of NUAK2 in lymph node metastases and prognosis. Results NUAK2 belongs to the AMP-activated protein kinase (AMPK) family, with its members controling protein metabolism, polarity, and overall cellular homeostasis actin cytoskeleton organization. The effect of NUAK2 variant expression in breast cancer metastases remains unknown. In this study, it was found that the expression level of NUAK2 was significantly higher in 5 paired metastatic lymph node than that in primary cancer, using Affymetrix GeneChip® Human Transcriptome Array 2.0. From online public database, higher NUAK2 expression level was markedly associated with better metastasis-free survival in breast cancer patients. The effects of NUAK2 on proliferation were investigated by CCK-8 and IncuCyte ZOOM™ assay, respectively. The results demonstrated that knockdown NUAK2 was able to significantly inhibit the proliferation of breast cancer cells. However, for cancer metastases, it was demonstrated that knock-down of NUAK2 significantly accelerated the migration of breast cancer cells, while overexpression of NUAK2 notably inhibited breast cancer cell migration. When promoting proliferation of breast cancer cells, NUAK2 might involve signal pathways of cell cycle. As for the anti-metastasis function, it would regulate cell adhesion, cell motility and microenvironment. Conclusion: Our in vitro study demonstrated that NUAK2 has shown reverse effects of tumor cell proliferation and invasion. The interaction of NUAK2 and cancer cells could be an important role in cell proliferation involved in cell cycle signal pathway, and modulate cell mobility by inducing cell-cell detachment. Its molecular mechanism of dual effect in breast cancer need further investigation. Citation Format: Wang Jia, XUE Jingyan, Hou Jianjing, Xiu Bingqiu, Li Lun, Chi Yayun, Wu Jiong. Functional duality of NUAK2 in breast cancer metastases [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P3-01-21.
Impact of rapid microbial identification on clinical outcomes in bloodstream infection: the RAPIDO randomized trial
Authors: A. MacGowan, S. Grier, M. Stoddart, R. Reynolds, C. Rogers, K. Pike, H. Smartt, M. Wilcox, P. Wilson, M. Kelsey, J. Steer, F.K. Gould, J.D. Perry, R. Howe, M. Wootton
Journal: Clinical Microbiology and Infection (2020)
CircPTK2 (hsa_circ_0005273) as a novel therapeutic target for metastatic colorectal cancer
Authors: Hongbao Yang, Xiaobo Li, Qingtao Meng, Hao Sun, Shenshen Wu, Weiwei Hu, Guilai Liu, Xianjing Li, Yong Yang, Rui Chen
Journal: Molecular Cancer (2020)

As a novel class of noncoding RNAs, circRNAs have been recently identified to regulate tumorigenesis and aggressiveness. However, the function of circRNAs in colorectal cancer (CRC) metastasis remains unclear. We aimed to identify circRNAs that are upregulated in CRC tissues from patients and study their function in CRC metastasis.
Guidance needed for singlehanded GPs to deal with covid-19 pandemic
Authors: Adrian O'Dowd
Journal: BMJ (2020)

Guidance needed for singlehanded GPs to deal with covid-19 pandemicAdrian O'Dowd London Singlehanded GPs are worried about what will happen to their practice patients if they themselves become ill with the covid-19 virus and have to self-isolate, it has emerged.The situation has been highlighted by a case of a husband and wife GP, both older than 70, who each have their own singlehanded practices in Nottingham.
Hydrogen Sulfide Alleviates Liver Injury Through the S‐Sulfhydrated‐Kelch‐Like ECH‐Associated Protein 1/Nuclear Erythroid 2–Related Factor 2/Low‐Density Lipoprotein Receptor–Related Protein 1 Pathway
Authors: Shuang Zhao, Tianyu Song, Yue Gu, Yihua Zhang, Siyi Cao, Qing Miao, Xiyue Zhang, Hongshan Chen, Yuanqing Gao, Lei Zhang, Yi Han, Hong Wang, Jun Pu, Liping Xie, Yong Ji
Journal: Hepatology (2020)

Background and Aims Protein S‐sulfhydration mediated by H 2 S has been shown to play important roles in several diseases. However, its precise role in liver disease and the related mechanism remain unclear. Approach and Results We showed that in streptozotocin (STZ)–treated and high‐fat diet (HFD)–treated low‐density lipoprotein receptor–negative (LDLr −/− ) mice, the H 2 S donor GYY4137 ameliorated liver injury, decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, mitigated lipid deposition, and reduced hepatocyte death. Strikingly, S‐sulfhydration of Kelch‐like ECH‐associated protein 1 (Keap1) was decreased in the livers of patients with fatty liver under diabetic conditions. In STZ+HFD‐treated LDLr −/− mice and in high glucose–treated and oxidized low‐density lipoprotein (ox‐LDL)–treated primary mouse hepatocytes, the GYY4137‐mediated increase in Keap1 S‐sulfhydration induced nuclear erythroid 2‐related factor 2 (Nrf2) dissociation from Keap1, which enhanced the nuclear translocation of Nrf2 itself and the consequent expression of antioxidant proteins. Keap1 Cys151 mutation significantly reduced Keap1 S‐sulfhydration and abolished the hepatoprotective effects of H 2 S both in vivo and in vitro . Nrf2 deficiency inhibited the H 2 S‐induced beneficial impacts in Nrf2 −/− mice. Similarly, in CCl 4 ‐stimulated mice, GYY4137 increased Keap1 S‐sulfhydration, improved liver function, alleviated liver fibrosis, decreased hepatic oxidative stress, and activated the Nrf2 signaling pathway; and these effects were abrogated after Keap1 Cys151 mutation. Moreover, H 2 S increased the binding of Nrf2 to the promoter region of LDLr‐related protein 1 ( Lrp1 ) and consequently up‐regulated LRP1 expression, but these effects were disrupted by Keap1 Cys151 mutation. Conclusions H 2 S‐mediated Keap1 S‐sulfhydration alleviates liver damage through activation of Nrf2. Hence, administration of exogenous H 2 S in the form of the H 2 S donor GYY4137 may be of therapeutic benefit in the context of concurrent hyperlipidemia and hyperglycemia–induced or CCl 4 ‐stimulated liver dysfunction.
Organ donation: the clock is ticking for public awareness
Authors: Faraz Mughal, Azeem Majeed
Journal: BMJ (2020)

Diaryliodonium salts are useful arylating reagents that have been exploited widely.In this Communication, we demonstrate that heating diphenyliodonium triflate in the solvent DMSO leads to an unexpected arylation reaction.It is postulated that arylation of DMSO at oxygen, followed by a thia-Sommelet-Hauser rearrangement, leads to the formation of 2-thiomethylphenols.More substituted diaryliodonium salts and cyclic diaryliodonium salts are shown to be more stable and less likely to react with DMSO.In conclusion, when using iodonium salts dissolved in DMSO, beware of side-reactions.
Comprehensive Genomic Analysis of Translocation Renal Cell Carcinoma Reveals Copy-Number Variations as Drivers of Disease Progression
Authors: Julian Marcon, Renzo G. DiNatale, Alejandro Sanchez, Ritesh R. Kotecha, Sounak Gupta, Fengshen Kuo, Vladimir Makarov, Amar Sandhu, Roy Mano, Andrew W. Silagy, Kyle A. Blum, Daniel E. Nassau, Nicole E. Benfante, Michael V. Ortiz, Maria I. Carlo, Timothy A. Chan, Robert J. Motzer, Martin H. Voss, Jonathan Coleman, Paul Russo, Victor Reuter, A. Ari Hakimi, Ed Reznik
Journal: Clinical Cancer Research (2020)

Translocation renal cell carcinoma (tRCC) is a rare, aggressive renal cell carcinoma (RCC) subtype. There is currently limited understanding on the role of molecular alterations in the pathogenesis and progression of these tumors. We investigated the association between somatic alterations and clinical outcomes in two independent cohorts profiled using DNA sequencing.
Ski racers’ understanding of sports-related concussion and its management: are contemporary findings and clinical recommendations reaching the target audience, the racers themselves?
Authors: Natalie Maxwell, Lucy Redhead, Evert Verhagen, Jörg Spörri
Journal: British Journal of Sports Medicine (2020)

In the past two decades, there has been a revolution in literature relating to the epidemiology, aetiology, early recognition, prevention, diagnosis and treatment of sports-related concussion (SRC). This research has resulted in the publication of five international concussion in sport consensus statements.1 Are those recommendations impacting on pitch-side rulings and contributing to the real-life management of SRC? We ask: ‘Is evidence-based SRC information reaching those it concerns the most, i.e. the athletes?’2

Within alpine ski racing, SRC is the most frequent head injury.3 The International Ski Federation (FIS) has established SRC return-to-play guidelines,4 but athletes aim to dodge the guidelines by under-reporting their symptoms (eg, persistent headache, blurred vision),5 possibly resulting in premature return to sport. Besides this, athlete adherence to such guidelines may be impeded by insufficient understanding of the potential long-term consequences of SRC (eg, increased risk of reinjury, prolonged recovery from subsequent concussions, mild cognitive impairment or depression).

To better understand alpine ski racing athletes’ perceptions, beliefs and experiences of concussion, we conducted a qualitative interview study with 11 participants. These 11 participants were representatives from a world-leading ski association comprising 88 athletes. The underlying …
Abstract P1-04-01: Breast cancer patient derived scaffolds as a platform for studying cancer promoting properties of the microenvironment
Authors: Elena Garre, Anna Gustafsson, Maria del Carmen Leiva-Arrabal, Paul Fitzpatrick, Pernilla Gregersson, Anders Ståhlberg, Göran Landberg
Journal: Cancer Research (2020)

Abstract Despite great efficiencies of today’s anti-cancer therapies in breast cancer medicine, recurrences and metastasis still remains a major challenge. The tumor initiating population of cancer stem cells (CSC) is believed to promote metastasis and drug resistance, suggesting that selectively targeting CSC and their niche may be a potential beneficial therapeutic strategy. However, specific driving factors in the microenvironment influencing the CSC niche remains unknown. To study the influence of specific cancer microenvironments, we have developed a novel three-dimensional cell culture platform, using cell-free patient derived scaffolds (PDS) from decellularized breast cancer tumors. We confirmed that our novel patient derived scaffold (PDS) recapitulates the native microenvironment where the cancer cells originate and, retains their biological properties. Our data demonstrate that culturing breast cancer cell lines in PDSs promotes CSC and EMT-like properties and decreases proliferation, similarly to in vivo conditions. Additionally, global gene expression profiling revealed that PDS cultures exhibit transcriptional patterns more similar to patient derived xenografts than to traditional monolayer cultures. Moreover, we analyzed the expression of cells cultured in 108 PDSs made from breast cancer tumors with clinical follow-up data, using a gene panel representative of relevant breast cancer related processes. This revealed that expression changes of several EMT markers and other CSC related genes in the PDS cultured cancer cells were correlated to clinical parameters such as grade, lymph node metastasis, patient survival and disease recurrences. Altogether, our data shows that PDS can reveal unique additional information about the malignancy-inducing properties of specific tumor microenvironments, potentially provides a complementary diagnosis tool for breast cancer tumors in vitro, and is a promising platform for drug screening of anti-cancer therapies. Citation Format: Elena Garre, Anna Gustafsson, Maria del Carmen Leiva-Arrabal, Paul Fitzpatrick, Pernilla Gregersson, Anders Ståhlberg, Göran Landberg. Breast cancer patient derived scaffolds as a platform for studying cancer promoting properties of the microenvironment [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P1-04-01.
Dupilumab improves clinical manifestations, symptoms, and quality of life in adult patients with chronic nodular prurigo
Authors: Andrea Chiricozzi, Martina Maurelli, Niccolò Gori, Giuseppe Argenziano, Clara De Simone, Giulia Calabrese, Giampiero Girolomoni, Ketty Peris
Journal: Journal of the American Academy of Dermatology (2020)
Congenital Dislocation of the Knee
Authors: R. William Provenzano
Journal: New England Journal of Medicine (1947)

ALTHOUGH congenital dislocation is extremely rare, it is the most frequent congenital deformity of the knee. In recent years the occurrence and the treatment of this lesion have received considerable attention,1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 but to date an etiologic grouping has not been presented. It is the purpose of this paper to formulate an etiologic classification of congenital dislocations of the knee, to review the various cases reported to determine the incidence in relation to hereditary tendencies, sex and types of dislocations, to discuss the numerous treatments prescribed and to present the case of a patient treated by simple conservative manipulation and early . . .
Abstract P1-20-28: Intraoperative margin status in breast conserving surgery - A novel 3D evaluation using tomosynthesis
Authors: Bothaina Nakad, Ibrahim Matter, Paula Simovici, Daria Milevsky, Oksana Greg, Gideon Sroka, Asia Brodsky
Journal: Cancer Research (2020)

Abstract Background: Margin status remains a debatable issue in breast cancer surgery, accounting for a 10-40% re-excision rate. Accurate intraoperative assessment of margin status enables one step surgery, lowers re-excision rates and offers better surgical and cosmetic outcomes for the patient. We present a novel 3D intraoperative evaluation using Screen Vision and Computed Tomosynthesis. Methods: Our standard suture-guided orientations for a lumpectomy in 36 patients (SG group) undergoing BCS was compared to the new 3D orientation using the Screen Vision, a plastic box, with a two-dimensional axis system combined with three-dimensional tomosynthesis, in the first 26 patients operated with this technique (3D group). We defined clear margins as “no ink on tumor” for invasive carcinoma, and more than 2 mm distance for ductal carcinoma in situ. The margin status and the re-excision rates were recorded, for both benign and malignant lesions. Results: The margin-expansion rate in the first operation according to intra-operative margin status imaging, was 6/36 (16%) in the SG group compared to 9/26 (34%) in the 3D group. Additional margin-expansion according to clinical suspect in spite of negative imaging status was performed in 14/36 (28%) in the SG group and 8/26 (30%) in the 3D group. The re-lumpectomy rate was 4/36 (11%) in the SG group and 2/26 (7%) in the 3D group. Conclusions: This method of combined Screen Vision and computed tomosynthesis presents easier orientation of surgical specimens, with better intra-operative analysis of surgical margins. It might have a role in improving intra-operative margin status imaging, with subsequent lowering of re-lumpectomy rates. Citation Format: Bothaina Nakad, Ibrahim Matter, Paula Simovici, Daria Milevsky, Oksana Greg, Gideon Sroka, Asia Brodsky. Intraoperative margin status in breast conserving surgery - A novel 3D evaluation using tomosynthesis [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P1-20-28.