tbuckley/cabot-search huggingface dataset:
Measurement of Gamma Glutamyl Transferase to Determine Risk of Liver Transplantation or Death in Patients With Primary Biliary Cholangitis
Authors: Alessio Gerussi, Davide Paolo Bernasconi, Sarah Elisabeth O'Donnell, Willem J. Lammers, Henk Van Buuren, Gideon Hirschfield, Harry Janssen, Christophe Corpechot, Anna Reig, Albert Pares, Pier Maria Battezzati, Massimo Giovanni Zuin, Nora Cazzagon, Annarosa Floreani, Frederik Nevens, Nikolaos Gatselis, George Dalekos, Marlyn J. Mayo, Douglas Thorburn, Tony Bruns, Andrew L. Mason, Xavier Verhelst, Kris Kowdley, Adriaan van der Meer, Grazia Anna Niro, Benedetta Terziroli Beretta-Piccoli, Marco Marzioni, Luca Saverio Belli, Fabio Marra, Maria Grazia Valsecchi, Keith D. Lindor, Pietro Invernizzi, Bettina E. Hansen, Marco Carbone
Journal: Clinical Gastroenterology and Hepatology (2020)
Optimum Blood Pressure in Patients With Shock After Acute Myocardial Infarction and Cardiac Arrest
Authors: Koen Ameloot, Pekka Jakkula, Johanna Hästbacka, Matti Reinikainen, Ville Pettilä, Pekka Loisa, Marjaana Tiainen, Stepani Bendel, Thomas Birkelund, Ann Belmans, Pieter-Jan Palmers, Eline Bogaerts, Robin Lemmens, Cathy De Deyne, Bert Ferdinande, Matthias Dupont, Stefan Janssens, Joseph Dens, Markus B. Skrifvars
Journal: Journal of the American College of Cardiology (2020)
Rasburicase and Glucose 6-Phophaste Dehydrogenase: Clinical Utility in the Management of Acute Leukemia Related Hyperuricemia
Authors: Walid Mohamedali, Jesus Vera Aguilera, Ryan M. Carr, Mythri Mudireddy, Abhishek Mangaonkar, Jonas Paludo, Kebede H. Begna
Journal: Blood (2017)
Abstract B37: Menin and EWS/FLI1 activate serine biosynthesis via ATF4 in Ewing sarcoma
Authors: Jennifer A. Jiménez, Ramon Ocadiz Ruiz, April Apfelbaum, Abhijay Kumar, Samuel Kerk, Jolanta Grembecka, Costas A. Lyssiotis, Elizabeth R. Lawlor
Journal: Cancer Research (2020)

Abstract Ewing sarcoma (ES) is an aggressive bone and soft-tissue tumor, most commonly driven by the EWS/FLI1 fusion oncogene. Previous work has revealed that the scaffolding protein menin functions to promote ES tumorgenicity. Additionally, our recent studies revealed a previously undescribed role for menin in the activation of the serine biosynthetic pathway (SSP), a critical metabolic pathway that is aberrantly activated in many human cancers. The transcription factor ATF4 has been identified in other tumor types as a master transcriptional regulator of the SSP. Furthermore, regulation of the SSP by EWS/FLI1 has been described, although the mechanism of this regulation remains unknown. The biologic functions of menin are largely determined by its protein-binding partners, the best characterized of which is MLL in epigenetic trithorax complexes. In the current study, we are investigating the mechanistic link between menin, EWS/FLI1, and SSP hyperactivation to test the hypothesis that, in the context of ES, these transcriptional regulators cooperate to promote metabolic reprogramming via ATF4. Through gain- and loss-of-function studies, our results show that ATF4 regulates the SSP in ES cells, and that loss of ATF4 impairs ES cell proliferation. Additionally, both pharmacologic inhibition with the menin:MLL interaction inhibitor, MI-503, and doxycycline-inducible shRNA knockdown of menin lead to loss of ATF4, coincident with loss of SSP expression. Knockdown of EWS/FLI1 similarly results in loss of both ATF4 and SSP expression. Interrogation of publicly available ChIP-seq data further shows enrichment for EWS/FLI1 binding at the ATF4 gene promoter that is lost with EWS/FLI1 knockdown, and ChIP-qPCR shows menin binding at the ATF4 gene promoter in association with H3K4me3 enrichment. ChIP-qPCR also shows enrichment of ATF4 binding at SSP gene promoters, which is diminished upon menin inhibition with MI-503. Preliminary studies suggest that ATF4 overexpression may rescue the effects of menin loss of function on the SSP. Together these findings support the hypothesis that menin hijacks the SSP via aberrant activation of ATF4, and that this depends on cooperation with EWS/FLI1. Ongoing studies are assessing whether this is mediated by trithorax-dependent or -independent functions of menin. Citation Format: Jennifer A. Jiménez, Ramon Ocadiz Ruiz, April Apfelbaum, Abhijay Kumar, Samuel Kerk, Jolanta Grembecka, Costas A. Lyssiotis, Elizabeth R. Lawlor. Menin and EWS/FLI1 activate serine biosynthesis via ATF4 in Ewing sarcoma [abstract]. In: Proceedings of the AACR Special Conference on the Advances in Pediatric Cancer Research; 2019 Sep 17-20; Montreal, QC, Canada. Philadelphia (PA): AACR; Cancer Res 2020;80(14 Suppl):Abstract nr B37.
International ITP Registry with Focus on the Asia Pacific Region: Update on Preliminary Findings of Epidemiological and Clinical Data
Authors: Beng H Chong, Jong-Wook Lee, Jun Ho Jang, Tzeon-Jye Chiou, Lai Heng Lee, Osman Ilhan, Pantep Angchaisuksiri, Ai-Sim Goh, Soo Chin Ng, Shinichiro Okamoto, Kenny Mauricio Galvez, Sarah Davidson
Journal: Blood (2017)
Expanding Our Understanding of Ovarian Cancer Risk: The Role of Incomplete Pregnancies
Authors: Alice W Lee, Stacey Rosenzweig, Ashley Wiensch, Susan J Ramus, Usha Menon, Aleksandra Gentry-Maharaj, Argyrios Ziogas, Hoda Anton-Culver, Alice S Whittemore, Weiva Sieh, Joseph H Rothstein, Valerie McGuire, Nicolas Wentzensen, Elisa V Bandera, Bo Qin, Kathryn L Terry, Daniel W Cramer, Linda Titus, Joellen M Schildkraut, Andrew Berchuck, Ellen L Goode, Susanne K Kjaer, Allan Jensen, Susan J Jordan, Roberta B Ness, Francesmary Modugno, Kirsten Moysich, Pamela J Thompson, Marc T Goodman, Michael E Carney, Jenny Chang-Claude, Mary Anne Rossing, Holly R Harris, Jennifer Anne Doherty, Harvey A Risch, Lilah Khoja, Aliya Alimujiang, Minh Tung Phung, Katharine Brieger, Bhramar Mukherjee, Paul D P Pharoah, Anna H Wu, Malcolm C Pike, Penelope M Webb, Celeste Leigh Pearce
Journal: JNCI Journal of the National Cancer Institute (2020)

Parity is associated with decreased risk of invasive ovarian cancer; however, the relationship between incomplete pregnancies and invasive ovarian cancer risk is unclear. This relationship was examined using 15 case-control studies from the Ovarian Cancer Association Consortium (OCAC). Histotype-specific associations, which have not been examined previously with large sample sizes, were also evaluated.
Risk of Systemic Adverse Events after Intravitreal Bevacizumab, Ranibizumab, and Aflibercept in Routine Clinical Practice
Authors: Maya H. Maloney, Stephanie R. Payne, Jeph Herrin, Lindsey R. Sangaralingham, Nilay D. Shah, Andrew J. Barkmeier
Journal: Ophthalmology (2020)
Abstract 12040: Volume of Primary Angioplasty Procedures and Mortality in Relation to Low Population Density After Acute Myocardial Infarction
Authors: Yasushi Matsuzawa, Masaaki Konishi, Yusuke Saigusa, Masataka Taguri, Masaomi Gohbara, Toshiaki Ebina, Masami Kosuge, Kiyoshi Hibi, Kunihiro Nishimura, Michikazu Nakai, Yoshihiro Miyamoto, Satoshi Yasuda, Yoshihiko Saito, Naoki Nakayama, Ichiro Takeuchi, Kouichi Tamura, Kazuo Kimura
Journal: Circulation (2019)

Introduction: The low population density may associate with high mortality in acute myocardial infarction (AMI) patients. The impact of distance to hospital and hospital primary percutaneous corona...
Hypoxia, snail, and EMT in breast cancer
Authors: Katja Lundgren, Caroline Holm, Goran Landberg
Journal: Cancer Research (2007)

AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA

5361 

Snail is one of the master regulators of the epithelial-mesenchymal transition (EMT) implicated in key tumor biological processes such as invasion and metastasizing. Besides the importance of genetic alterations in tumor cells it is obvious that also the micro environment affects the behavior of tumor cells. Hypoxia is one element in the tumor micro environment potentially affecting many important processes linked to clinical aggressiveness of a tumor. We therefore set out to investigate the regulation of EMT in hypoxia, by determining the role of the E-cadherin transcriptional repressor Snail, in breast cancer cell lines and primary breast tumors. Cell lysates and pellets from different time points of hypoxic exposure of the breast cancer cell lines MCF7, T47D and CAMA1 were analyzed by western blotting and immunohistochemistry, using antibodies for Snail and E-cadherin. The Snail antibody was validated for western blotting and immunhistochemistry by analyzes of cell lines transiently overexpressing Snail. At 0.1 % O2 (severe hypoxia) there was a clear increase in Snail protein content after approximately four hours in all three breast cancer cell lines. There was further a decrease in E-cadherin protein in MCF7 cells but no difference in migratory properties of these cell lines grown in hypoxia compared to normoxia. In order to test how the cell lines responded to a Snail increase, we overexpressed Snail in MCF7 and T47D cells. Interestingly, both cell lines were more migratory when overexpressing Snail but only MCF7 cells expressed lower levels of E-cadherin compared to control transfected cells. Further, transient siRNA transfection against Snail resulted in a slightly decreased migration compared to control transfection in MCF7 cells. These results indicate that hypoxia induces Snail but no migratory phenotype suggesting that hypoxic cells are partially pushed towards EMT. To further validate the importance of Snail in breast cancer and also delineate any potential links to clinical behaviors and HIF1α protein content, we analyzed a material of 350 primary breast cancer samples arranged in a tissue micro array. There was no direct link between Snail expression and HIF1α protein content but Snail expression correlated positively to lymph node status. This supports that increased expression of Snail protein in primary breast cancer might be important for the ability of tumors to metastasize, possibly through EMT.
SAT0306 SEMIQUANTITATIVE AND QUANTITATIVE ANALYSIS OF LUNG CT IN THE ASSESSMENT OF INTERSTITIAL LUNG DISEASE IN IDIOPATHIC INFLAMMATORY MYOPATHIES WITH A FOCUS ON ANTISYNTHETASE.
Authors: S. Barsotti, C. Roncella, A. Valentini, L. Cavagna, R. Castellana, E. Cioffi, A. Tripoli, D. Caramella, B. Bartholmai, R. Neri, F. Falaschi, C. Romei, M. Mosca
Journal: Annals of the Rheumatic Diseases (2020)

Background: Interstitial lung disease (ILD), is common in patients with idiopathic inflammatory myopathies (IIM) and strongly impact on patients’ morbidity and mortality. Patients with anti-aminoacyl-transfer RNA-synthetases (anti-ARS) antibodies are associated with an increased risk of ILD. Objectives: Defining the radiological characteristics of IIM patients, with special focus on serological groups, through qualitative, semiquantitative and quantitative analysis of lung CT. Methods: This was a prospective study conducted from 2016 to 2019. Ninety-eight IIM patients (35 men, 63 women) were included. Myositis specific autoantibodies (MSA) were assessed with Myositis Prophyle III (Euroimmune, Lubeck). Each patient had a baseline CT; the total score of Warrick (WS) was obtained at semiquantitative analysis. The radiological scores ILD% (interstitial lung disease %) and PVRS% (pulmonary vascular related structure) were the result of quantitative analysis in 61 patients (CALIPER). Pulmonary function tests (PFTs) included TLC%, FVC% and DLCO% (65 patients). The analysis was conducted in the whole group and divided in subgroups based on their MSA pattern: in particular anti-ARS (Group 1) and patients negative to MSA (Group 2) were analysed. Results: Positive correlations between ILD% and PVRS% (Rho=0.916; ρ=0.000), WS and ILD% (Rho=0.663; ρ=0.000) and WS and PVRS% (Rho=0.637; ρ&lt;0.001) were found. The most relevant inverse correlations were found between ILD% and DLCO% (Rho=-0.590; ρ=0.001), PVRS% and DLCO% (Rho=-0.549; ρ&lt;0.001) and WS and DLCO% (Rho=-0.471; ρ&lt;0.001). Statistically significant higher values of WS, ILD% and PVRS% were found in Group 1 (WS=15, ILD%=11 and PVRS%=3.5), compared to Group 2 (WS=2.5, ILD%=0.84 and PVRS%=2.2). NSIP pattern resulted dominant represented in the two groups (80% Group 1, 75% Group 2). No statistically significant differences of DLCO%, FVC% and TLCO% were found. Conclusion: The inverse correlations between the radiological scores and the functional data TLC% and DLCO% (ρ&lt;0.001) confirm the role of lung CT in the clinical management of ILD in IIM patients, and may represent a promising tool for clinical trials. For the first time anti-ARS and serological negative patients were defined through qualitative, semiquantitative and quantitative analysis of lung CT. Further study should be conducted in order to define the prognostic value of the quantitative analysis of lung CT in the follow up of IIM patients. Disclosure of Interests: None declared
Abstract 13507: Dk-Crush Stenting Increases the Risk of Incomplete Stent Apposition Compared With Culotte Stenting for the Treatment of Left Main Coronary Bifurcation Lesion: Analysis From Micro CT
Authors: Yutaka Hikichi, Kaito Matsubara, Kouta Sugiyama, Xiaodong Zhu, Yusuke Tsuboko, Yuki Matsuhashi, Yohei Inoue, Masahiro Natsuaki, Koichi Node, Kiyotaka Iwasaki
Journal: Circulation (2019)

Aims: There is no established method for a planned 2-stent strategy is deemed necessary for unprotected LM coronary artery disease. This study aimed to investigate the influence of stent implantati...
Association of a Serum Protein Signature With Rheumatoid Arthritis Development
Authors: Liam J. O’Neil, Victor Spicer, Irene Smolik, Xiaobo Meng, Rishi R. Goel, Vidyanand Anaparti, John Wilkins, Hani S. El‐Gabalawy
Journal: Arthritis & Rheumatology (2020)

The pathophysiologic events that precede the onset of rheumatoid arthritis (RA) remain incompletely understood. This study was undertaken to identify changes in the serum proteome that precede the onset of RA, with the aim of providing new insights into the pathogenic mechanisms that lead to its development.In a cohort of first-degree relatives of Indigenous North American RA patients, the SomaScan proteomics platform was used to determine the levels of 1,307 proteins in multiple longitudinal serum samples from 17 individuals who were followed up prospectively to the time of disease onset. Proteomic signatures from this group of individuals (designated the progressor group) were compared to those in a group of individuals who were considered at risk of developing RA, stratified as either positive (n = 63) or negative (n = 47) for anti-citrullinated protein antibodies (ACPAs) (designated the at-risk group). Machine learning was used to identify a protein signature that could accurately classify those individuals at highest risk of future RA development.A preclinical proteomic signature that differentiated RA progressors from at-risk individuals, irrespective of ACPA status, was identified (area under the curve 0.913, accuracy 91.2%). Importantly, the predictive preclinical proteomic signature was present not only in serum samples obtained close to the onset of RA, but also in serum samples obtained a median of 30.9 months prior to onset. Network analysis implicated the activation of Toll-like receptor 2 and production of tumor necrosis factor and interleukin-1 as key events that precede RA progression.Alterations in the serum proteome in the preclinical phase of RA can emerge years prior to the onset of disease. Our findings suggest that the serum proteome provides a rich source of proteins serving both to classify at-risk individuals and to identify molecular pathways involved in the development of clinically detectable RA.
AB0188 COMPARISON OF COMPOSITE INDICES FOR DETECTING REMISSION ON ULTRASOUND
Authors: R. Fakhfakh, N. El Amri, K. Baccouche, H. Zeglaoui, E. Bouajina
Journal: Annals of the Rheumatic Diseases (2020)

Background: Several studies have shown the greater sensitivity of ultrasound (US) to detect B-mode synovitis and synovial Doppler activity in a high percentage of rheumatoid Arthritis (RA) patients in clinical remission, assessed by different composite indices. Objectives: The aim of the study was to compare the accuracy of composite indices to detect remission in ultrasound B-mode and power Doppler (PD) in RA patients that are in remission according to the DAS28 ESR. Methods: Cross-sectional study including patients with RA in clinical remission defined by: DAS28ESR ≤ 2.6, without disease flare or changes in therapy in the previous 6 months. Each patient underwent B-mode and PD assessments of 36 joints and 20 tendons in the Rheumatology Department over a period of 6 month. B-mode and PD signal for synovitis and tenosynovitis were defined according to the Outcome Measures in Rheumatology Clinical Trials (OMERACT). A global score for B-mode and a global score for PD signal were calculated for each patient. The DAS28, CDAI, SDAI and the Boolean 2010 ACR/EULAR remission criteria were compared. Results: Thirty two patients were enrolled, the mean age was 53.7±13.4 and the sex ratio M/F was 0.3. The mean disease duration was 15.0 years ± 8.8. According to the SDAI, 68.8% of patients were in remission. These were lower for the CDAI (62.5%) and the Boolean criteria (23.3%). Synovial hypertrophy and tenosynovitis in B mode was detected in 100% with the Boolean remission criteria, in 93.8% with a DAS28, in 90.9% with a SDAI ≤ 3.3 and in 90% with a CDAI ≤ 2.8 (p&gt;0.05). The PD signal was detected in 62.5% with a DAS28, in 59.1% with a SDAI ≤ 3.3, in 57.1% with the Boolean remission criteria and in 55.1% with a CDAI≤ 2.8 (p&gt;0.05). The mean B-mode global score was higher for the DAS28 ESR (8.2±6) and lower for the Boolean remission criteria (6.2 ±5.4). For a CDAI ≤ 2.8, the mean global score for B-mode was 7.6 ±5.9 and for a SDAI ≤ 3.3, it was 7.4±5.7. The median PD global score was similar for the DAS28, SDAI≤ 3.3 and Boolean remission criteria (1[0-12]). It was higher for a CDAI ≤ 2.8 (1.5 [0-12]). The global score for PD signal was correlated with DAS28 ESR (r: 0.42 p: 0.02). There were no significant correlations between the other indices and the mode B and PD global scores. Conclusion: The CDAI least detected subclinical synovitis and tenosynovitis in B mode and in power Doppler signal but it showed higher scores of power Doppler. References: [1]Merve Ozata Olmez, Esen Kasapoglu Gunal, Sibel Bakirci Ureyen and al. Comparison of composite indices with global synovitis score on ultrasound for detecting remission. Clinical Rheumatology 2017. doi.org/10.1007/s10067-017-3925-x Disclosure of Interests: None declared
Response to Open Peer Commentaries on “Partnering with Patients to Bridge Gaps in Consent for Acute Care Research”
Authors: Neal W. Dickert, A. Michelle Bernard, JoAnne M. Brabson, Rodney J. Hunter, Regina McLemore, Andrea R. Mitchell, Stephen Palmer, Barbara Reed, Michele Riedford, Raymond T. Simpson, Candace D. Speight, Tracie Steadman, Rebecca D. Pentz
Journal: The American Journal of Bioethics (2020)

"Response to Open Peer Commentaries on “Partnering with Patients to Bridge Gaps in Consent for Acute Care Research”." The American Journal of Bioethics, 20(8), pp. W12–W13
Targeted Delivery of CXCL9 and OX40L by Mesenchymal Stem Cells Elicits Potent Antitumor Immunity
Authors: Pan Yin, Liming Gui, Caihong Wang, Jingjing Yan, Min Liu, Lu Ji, You Wang, Bin Ma, Wei-Qiang Gao
Journal: Molecular Therapy (2020)

Major obstacles in immunotherapies include toxicities associated with systemic administration of therapeutic agents, as well as low tumor lymphocyte infiltration that hampers the efficacies. In this study, we report a mesenchymal stem cell (MSC)-based immunotherapeutic strategy in which MSCs specifically deliver T/natural killer (NK) cell-targeting chemokine CXCL9 and immunostimulatory factor OX40 ligand (OX40L)/tumor necrosis factor superfamily member 4 (TNFSF4) to tumor sites in syngeneic subcutaneous and azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced spontaneous colon cancer mouse models. This approach generated potent local antitumor immunity by increasing the ratios of tumor-infiltrating CD8+ T and NK cells and production of antitumor cytokines and cytolytic proteins in the tumor microenvironment. Moreover, it improved the efficacy of programmed death-1 (PD-1) blockade in a syngeneic mouse model and significantly suppressed the growth of major histocompatibility complex class I (MHC class I)-deficient tumors. Our MSC-based immunotherapeutic strategy simultaneously recruits and activates immune effector cells at the tumor site, thus overcoming the problems with toxicities of systemic therapeutic agents and low lymphocyte infiltration of solid tumors. Major obstacles in immunotherapies include toxicities associated with systemic administration of therapeutic agents, as well as low tumor lymphocyte infiltration that hampers the efficacies. In this study, we report a mesenchymal stem cell (MSC)-based immunotherapeutic strategy in which MSCs specifically deliver T/natural killer (NK) cell-targeting chemokine CXCL9 and immunostimulatory factor OX40 ligand (OX40L)/tumor necrosis factor superfamily member 4 (TNFSF4) to tumor sites in syngeneic subcutaneous and azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced spontaneous colon cancer mouse models. This approach generated potent local antitumor immunity by increasing the ratios of tumor-infiltrating CD8+ T and NK cells and production of antitumor cytokines and cytolytic proteins in the tumor microenvironment. Moreover, it improved the efficacy of programmed death-1 (PD-1) blockade in a syngeneic mouse model and significantly suppressed the growth of major histocompatibility complex class I (MHC class I)-deficient tumors. Our MSC-based immunotherapeutic strategy simultaneously recruits and activates immune effector cells at the tumor site, thus overcoming the problems with toxicities of systemic therapeutic agents and low lymphocyte infiltration of solid tumors.
T Follicular Regulatory (TFR) Cell Deficiency during Chronic Graft-Versus-Host Disease Is Improved By Low-Dose IL-2 Therapy
Authors: Edouard Forcade, Yusuke Kamihara, Haesook T Kim, Isabelle Douchet, John Koreth, Masahiro Hirakawa, Corey Cutler, Sarah Nikiforow, Vincent T Ho, Philippe Armand, Edwin Alyea, Patrick Blanco, Bruce Blazar, Joseph H. Antin, Robert J. Soiffer, Jerome Ritz
Journal: Blood (2017)
Abstract 17229: Underuse of Evidence-Based Guidelines for Incident Type 2 Diabetes in a Countywide Cohort: Outcomes Across Socioeconomic Strata at One Year
Authors: Bradi B Granger, Susan E Spratt, Matthew Phelan, Ursula Rogers, Benjamin A Goldstein
Journal: Circulation (2019)

Introduction: Diabetes affects>29 million Americans and is the seventh-leading cause of death in the United States. The use of guideline-based therapies improves outcomes, but actual adoption in cl...
Long-term placement of lumen-apposing metal stent after endoscopic ultrasound-guided duodeno- and jejunojejunal anastomosis for direct access to excluded jejunal limb
Authors: Gianfranco Donatelli, Fabrizio Cereatti, Andrea Spota, David Danan, Thierry Tuszynski, Jean-Loup Dumont, Serge Derhy
Journal: Endoscopy (2020)

Abstract Background Management of biliary disorders in patients with altered anatomy may be challenging. Endoscopic ultrasound (EUS)-guided gastrointestinal anastomosis using a lumen-apposing metal stent (LAMS) was introduced to allow endoscopic retrograde cholangiography (ERC) in such cases. However, the appropriate stent indwelling time remains uncertain. We report long-term LAMS deployment after duodenojejunal or jejunojejunal anastomosis (EUS-DJA) to allow endoscopic reinterventions in cases of recurrences. Methods 11 consecutive patients underwent EUS-DJA with long-standing LAMS between January 2017 and December 2018. Over a 12-month period, ERC treatment was carried out with multiple endoscopic sessions across the DJA. Results Technical success was 91 % (10/11) for EUS-DJA and 100 % for ERC. Four patients presented stricture recurrence at a mean of 489 days (standard deviation [SD] 31.7) after the end of ERC treatment. A novel ERC across the LAMS anastomosis was feasible in all cases. At a mean of 781 days (SD 253.1), all LAMS remained in place with no evidence of complications. Conclusion Long-term LAMS placement after EUS-DJA may be feasible and safe for direct access to the excluded limb.