Abstract 1711: Mechanical strain induces phenotypic changes in breast cancer cells and promotes immunosuppression in the tumor microenvironment
Authors: Yong Wang, Kayla F. Goliwas, Paige E. Severino, Kenneth Hough, Derek Van Vessem, Hong Wang, Sultan Tousif, Roy P. Koomullil, Andra R. Frost, Selvarangan Ponnazhagen, Joel L. Berry, Jessy S. Deshane
Journal: Cancer Research (2020)

Abstract Introduction: Breast cancer (BCa) occurs with a complex, three-dimensional microenvironment that involves heterogeneous biochemical and biophysical cues. Understanding how mechanical properties within the tumor microenvironment (TME) regulate breast cancer phenotype and immunosuppression is of great interest. Materials and Methods: BCa cells (MCF-7, MDA-MB-231 or 4T1.2) cultured to confluence on collagen coated FlexCell culture plates were subjected to 10% uniaxial cyclic/oscillatory strain at 0.3 Hz, or 10% constant strain, or no strain for 48 hours. They were isolated for analysis of proliferation (MTT assay and cell count by trypan blue), and migration (transwell and wound healing assay). Exosomes from conditioned media were isolated by differential centrifugation or using the Total Exosome Isolation kit. The purified exosomes were quantified by NanoSight and characterized by ImageStream. 5 × 105 4T1.2 cells or PKH67-labeled strained or control cells were injected into the mammary fat pad of BALB/c mice. Tumor volume was measured at the indicated time points after injection. Tumor-infiltrating immune cells and the internalization of exosomes were analyzed by flow cytometry on day 14 post implantation. In some experiments, on day 6 after tumor injection, 7.5 × 108 PHK67-labeled tumor cell-derived exosomes or PBS were injected into the tumor nodule. Tumor tissues were harvested for analysis of the internalization of exosomes by immune cells and tumor cells on days 2 and 8 after exosome injection. Results: We show that mechanical strain enhanced the proliferation and migration of BCa cells in vitro. Exosome concentrations produced by triple negative breast cancer (TNBC) cells were increased following exposure to oscillatory strain. Phenotyping exosomes by ImageStream showed that the percentages of CD81+PD-L1+ and CD63+PD-L1+ exosomes were increased after exposure to oscillatory strain. Using a syngeneic orthotopic mouse model of TNBC, we showed that preconditioning with mechanical strain increased tumor growth. The percentages of tumor-infiltrating monocytic myeloid-derived suppressor cells (M-MDSC) and recruited macrophages were increased while CD8+ T cells decreased in the TME of mice implanted with 4T1.2 cells preconditioned with oscillatory strain. Further, exosome internalizations by M-MDSC and recruited macrophages were elevated when tumor cells were preconditioned with oscillatory strain. Moreover, exosomes internalization by immune cells and tumor cells in TME were identified by PKH67 positive signals on days 2 and 8 after injection of PKH67-labeled exosomes into tumor nodules by flow cytometry analyses and confocal microscope imaging. Conclusions: Our data indicate that exposure to mechanical strain promotes invasive and pro-tumorigenic phenotypes in BCa, alters exosome production by BCa and induces immunosuppression in the TME. Citation Format: Yong Wang, Kayla F. Goliwas, Paige E. Severino, Kenneth Hough, Derek Van Vessem, Hong Wang, Sultan Tousif, Roy P. Koomullil, Andra R. Frost, Selvarangan Ponnazhagen, Joel L. Berry, Jessy S. Deshane. Mechanical strain induces phenotypic changes in breast cancer cells and promotes immunosuppression in the tumor microenvironment [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1711.