tbuckley/cabot-search huggingface dataset:
Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
Authors: Maegan L. Capitano, Nirit Mor-Vaknin, Anjan K. Saha, Scott Cooper, Maureen Legendre, Haihong Guo, Rafael Contreras-Galindo, Ferdinand Kappes, Maureen A. Sartor, Christopher T. Lee, Xinxin Huang, David M. Markovitz, Hal E. Broxmeyer
Journal: Journal of Clinical Investigation (2019)

The nuclear protein DEK is an endogenous DNA-binding chromatin factor regulating hematopoiesis. DEK is one of only 2 known secreted nuclear chromatin factors, but whether and how extracellular DEK regulates hematopoiesis is not known. We demonstrated that extracellular DEK greatly enhanced ex vivo expansion of cytokine-stimulated human and mouse hematopoietic stem cells (HSCs) and regulated HSC and hematopoietic progenitor cell (HPC) numbers in vivo and in vitro as determined both phenotypically (by flow cytometry) and functionally (through transplantation and colony formation assays). Recombinant DEK increased long-term HSC numbers and decreased HPC numbers through a mechanism mediated by the CXC chemokine receptor CXCR2 and heparan sulfate proteoglycans (HSPGs) (as determined utilizing Cxcr2-/- mice, blocking CXCR2 antibodies, and 3 different HSPG inhibitors) that was associated with enhanced phosphorylation of ERK1/2, AKT, and p38 MAPK. To determine whether extracellular DEK required nuclear function to regulate hematopoiesis, we utilized 2 mutant forms of DEK: one that lacked its nuclear translocation signal and one that lacked DNA-binding ability. Both altered HSC and HPC numbers in vivo or in vitro, suggesting the nuclear function of DEK is not required. Thus, DEK acts as a hematopoietic cytokine, with the potential for clinical applicability.
EFFICACY OF ESD IN LESIONS LOCATED IN DENTATE LINE. A PROSPECTIVE MULTICENTRIC COMPARATIVE STUDY
Authors: J Rodríguez-Sánchez, F Múgica, A Eduardo, JD Tasende, P Rosón, J De la Peña, Á Terán, A Herreros de Tejada, F Ramos, S Rodríguez, G Fernández Esparrach, A Del Pozo, C Dolz, H Uchima, A Álvarez, A Amoros, R Duran, H Cortés, JC Marín Gabriel
Journal: Endoscopy (2019)

Endoscopic submucosal dissection (ESD) is a well-established treatment for complex colorectal lesions. However, those which involve the dentate line (DL) up to 1 cm, have been traditional treated by transanal endoscopic microsurgery (TEM). Poor manoeuvrability and the presumable risk of bleeding might become ESD more difficult and unfeasible. Therefore, the lack of prospective comparative studies of ESD with this indication, makes it questionable.
Direct-acting antiviral therapy slows kidney function decline in patients with Hepatitis C virus infection and chronic kidney disease
Authors: Meghan E. Sise, Donald F. Chute, Yaa Oppong, Maya I. Davis, Joshua D. Long, Sakuni T. Silva, Nifasha Rusibamayila, Deborah Jean-Francois, Syeda Raji, Sophia Zhao, Ravi Thadhani, Raymond T. Chung
Journal: Kidney International (2019)
SELF-EXPANDABLE METALLIC STENTS (SEMS) IN ESOPHAGEAL VARICES POST BAND ULCER REFRACTORY BLEEDING, A RETROSPECTIVE STUDY
Authors: O Elbahr, A Kamal, A Alsebaey, M Amin, M Abassy, A Edris, A Nada, A Sabry, A el-Aleem Helal, AN Gad-Allah, M Abd Elaziz
Journal: Endoscopy (2019)

Evaluate efficacy of SEMS in control of variceal post band ulcer bleeding and 42 days survival outcome.
FELLOW INVOLVEMENT DURING COLONOSCOPY DOES NOT AFFECT EXAMINATION'S QUALITY INDICATORS IN A GREEK TERTIARY ENDOSCOPY FACILITY
Authors: P Gkolfakis, G Tziatzios, A Stamou, S Karamaroudis, K Grammatikos, K Miltiadou, P Gatos-Gatopoulos, K Triantafyllou
Journal: Endoscopy (2019)

To evaluate the effect of fellow's involvement during colonoscopy on examination's quality indicators.
Programmatic human papillomavirus testing in cervical cancer prevention in the Jujuy Demonstration Project in Argentina: a population-based, before-and-after retrospective cohort study
Authors: Silvina Arrossi, Melisa Paolino, Rosa Laudi, Juan Gago, Alicia Campanera, Oscar Marín, Cristina Falcón, Verónica Serra, Rolando Herrero, Laura Thouyaret
Journal: The Lancet Global Health (2019)

BackgroundHuman papillomavirus (HPV) testing for cervical cancer prevention was introduced in Argentina through the Jujuy Demonstration Project (2011–14). The programme tested women aged 30 years and older attending the public health system with clinician-collected HPV tests. HPV self-collection was introduced as a programmatic strategy in 2014. We aimed to evaluate the effectiveness of programmatic HPV testing to detect cervical intraepithelial neoplasia (CIN) of grade 2 or worse (CIN2+) in comparison with cytology-based screening.MethodsWe did a population-based, before-and-after retrospective cohort study using data from the National Cervical Cancer Prevention Program for the Jujuy province in northwest Argentina. We obtained data for the cytology-based screening period from Jan 1, 2010, until Dec 31, 2011, and for the HPV-based screening period from Jan 1, 2012, until Dec 31, 2014. The primary outcome was detection of histologically diagnosed CIN2+ among women aged 30 years and older. To assess the outcomes in all individuals included in the study, we used multivariable logistic regression and propensity score matching. The reach, effectiveness, adoption, implementation, and maintenance (RE-AIM) framework was used for the before-and-after analysis of programmatic dimensions.FindingsOf the 29 631 women who underwent cytology-based screening in 2010–11, CIN2+ was detected in 236 (0·8%) individuals. Of the 49 565 women HPV tested in 2012–14 (clinician-collected tests, n=44 700; self-collection tests, n=4865), 693 (1·4%; 658 clinician-collected tests; 35 self-collection tests) were found to have CIN2+ after the first round of screening. Compared with cytology-based screening, the odds ratio of being diagnosed with a CIN2+ lesion was 2·34 (95% CI 2·01–2·73; p<0·0010) with clinician-collected tests, and 1·08 (0·74–1·52; p=0·68) when screened with self-collection tests, after controlling for age and health insurance status. Screening coverage was similar in both periods (52·7% vs 53·2%); improvements of programmatic indicators were observed in the HPV testing period in relation to laboratory centralisation, lower overscreening (6·6% vs 0·0%), higher adherance to age recommendations (79·3% vs 98·8%), and a decrease of inadequate samples (3·6% vs 0·2%).InterpretationHPV testing in middle-income settings increases detection of CIN2+ lesions and allows for improvement of programmatic indicators. Evidence suggests that the introduction of HPV testing will accelerate the reduction of cervical cancer burden.FundingArgentinian National Cancer Institute and National Council of Scientific and Technologic Research.
Immune functions of follicular helper CD4+CXCR5+ T cells in human breast cancer
Authors: M. Langouo Fontsa, G. Noel, S. Garaud, A. de Wind, G. Van den Eynden, A. Boisson, C. Naveaux, D. Larsimont, M. Piccart, K. Willard-Gallo
Journal: Annals of Oncology (2019)

Background: Tumor-infiltrating lymphocytes (TIL) have been associated with good clinical outcomes in HER2-positive (HER2+) and triple-negative (TN) breast cancer (BC) patients. Recently, we found that in 60% of BC, TIL are organized in tertiary lymphoid structures (TLS) in the stroma. We further identified a CXCL13-producing CD4+T follicular helper (Tfh) cell subpopulation associated with positive clinical outcomes in BC. We aimed to investigate how CD4+ Tfh cells, expressing the CXCL13 receptor CXCR5, contribute to immune response in BC TLS. Methods: Prospectively collected fresh primary BC tissues were dissociated without enzymes to separate tumor supernatants and TIL and used for Immunoglobulin (Ig)/cytokines quantification and flow cytometric analysis/sorting. Matching formalin-fixed paraffin-embedded (FFPE) were used for spatial analysis. Results: In our BC cohort, around 15% CD4+ TIL, 13% CD8+ TIL and >95% B cell TIL express CXCR5 while confocal microscopy reveals that they colocalized in BC TLS. In some BC Tfh TIL (as activated tonsillar Tfh) express ICOS and PD-1 suggesting they are activated. RNA analysis on sorted Tfh TIL detected high expression of IL-21, INFg, and CXCL13. In an in vitro assay where Tfh TIL were activated with splenic B cells revealed that only ICOS+PD-1+Tfh TIL from TN/HER2+ BC are capable of inducing Ig secretion by B cells. mRNA analysis of sorted ICOS+PD-1+Tfh TIL confirms an activated, functional nature for the former with high levels of mRNA expression of IL-21, INFg, and CXCL13. We found that CXCR5+CD8 TIL expressing ICOS and PD-1 are related to high expression of CCL4, FASL, granzyme B and IFNγ. Indeed multiplex IHC analyses confirm the presence of the later in close contact with functional Tfh and B TIL. A high correlation between the presence of functional Tfh with activated CD8 TIL and with Ig secreted in the NANT from TN/HER2+ BC was observed. Conclusions: We describe two distinct population of Tfh TIL. The CXCL13-producing Tfh that may recruit the CXCR5+Tfh TIL in BC TLS. Functional Tfh TIL probes to guide humoral and cytotoxic anti-tumor immunity in TLS. These data shed light on anti-tumor immune responses taking place in the TLS, whose functional activities may have important treatment implications, particularly for immunotherapy in BC. Legal entity responsible for the study: Karen Willard-Gallo. Funding: Fonds National de Recherche Scientifique Belgium/ Télévie. Disclosure: All authors have declared no conflicts of interest.
RADIOLOGICALLY VS ENDOSCOPICALLY-PLACED GASTROSTOMY FEEDING TUBES: AN AUDIT OF CURRENT PRACTICE AND CLINICAL OUTCOMES IN A LARGE, MULTI-SITE UK NHS TRUST
Authors: S Pannick, L Hicks, J Kim, Z Velji, K Colucci, A Wright, W Howson
Journal: Endoscopy (2019)

The optimum method for gastrostomy tube placement is unclear. A Cochrane review found insufficient evidence to promote either percutaneous endoscopic gastrostomy (PEG) or radiologically inserted gastrostomy (RIG). Here, we audit practice and clinical outcomes for gastrostomies across our three acute hospitals.
The mutational burden of therapy-related myeloid neoplasms is similar to primary myelodysplastic syndrome but has a distinctive distribution
Authors: Deepak Singhal, Li Yan A. Wee, Monika M. Kutyna, Rakchha Chhetri, Joel Geoghegan, Andreas W. Schreiber, Jinghua Feng, Paul P.-S. Wang, Milena Babic, Wendy T. Parker, Smita Hiwase, Suzanne Edwards, Sarah Moore, Susan Branford, Teodora Kuzmanovic, Nimit Singhal, Raghu Gowda, Anna L. Brown, Peer Arts, Luen B. To, Peter G. Bardy, Ian D. Lewis, Richard J. D’Andrea, Jaroslaw P. Maciejewski, Hamish S. Scott, Christopher N. Hahn, Devendra K. Hiwase
Journal: Leukemia (2019)
Unplanned 30-Day Readmission Risk Among Patients with Acute Myocardial Infarction: A Report from TRANSLATE-ACS
Authors: Connie N. Hess, Tracy Y. Wang, Lisa Kaltenbach, Emily Honeycutt, John C. Messenger, William T. Smith, Marjorie E. Zettler, Mark B. Effron, Timothy D. Henry, Eric D. Peterson, Gregg C. Fonarow
Journal: Circulation (2012)
ABCB1 SNP predicts outcome in patients with acute myeloid leukemia treated with Gemtuzumab ozogamicin: a report from Children’s Oncology Group AAML0531 Trial
Authors: Roya Rafiee, Lata Chauhan, Todd A. Alonzo, Yi-Cheng Wang, Ahlam Elmasry, Michael R. Loken, Jessica Pollard, Richard Aplenc, Susana Raimondi, Betsy A. Hirsch, Irwin D. Bernstein, Alan S. Gamis, Soheil Meshinchi, Jatinder K. Lamba
Journal: Blood Cancer Journal (2019)

Abstract Gemtuzumab-ozogamicin (GO), a humanized-anti-CD33 antibody linked with the toxin-calicheamicin-γ is a reemerging and promising drug for AML. Calicheamicin a key element of GO, induces DNA-damage and cell-death once the linked CD33-antibody facilitates its uptake. Calicheamicin efflux by the drug-transporter PgP-1 have been implicated in GO response thus in this study, we evaluated impact of ABCB1 -SNPs on GO response. Genomic-DNA samples from 942 patients randomized to receive standard therapy with or without addition of GO (COG-AAML0531) were genotyped for ABCB1-SNPs. Our most interesting results show that for rs1045642, patients with minor-T-allele (CT/TT) had better outcome as compared to patients with CC genotype in GO-arm (Event-free survival-EFS: p = 0.022; and risk of relapse-RR, p = 0.007). In contrast, no difference between genotypes was observed for any of the clinical endpoints within No-GO arm (all p &gt; 0.05). Consistent results were obtained when genotype groups were compared by GO and No-GO arms. The in vitro evaluation using HL60-cells further demonstrated consistent impact of rs1045642-T-allele on calicheamicin induced DNA-damage and cell-viability. Our results show the significance of ABCB1 SNPs on GO response in AML and warrants the need to investigate this in other cohorts. Once validated, ABCB1 -SNPs in conjunction with CD33-SNPs can open up opportunities to personalize GO-therapy.
Abstract 17384: Increased Estimated Cardiac Output and Functional Improvement in Fetuses in Response to Laser Therapy for Twin-Twin Transfusion Syndrome
Authors: Shiraz A Maskatia, Rodrigo Ruano, Abdollah A Shamshirsaz, Pouya Javadian, Joshua A Kailin, Michael A Belfort, Carolyn A Altman, Nancy A Ayres
Journal: Circulation (2013)

BACKGROUND: Twin-twin transfusion syndrome (TTTS) is thought to involve placental vascular anastomoses that cause a hyperdynamic state in the donor fetus and volume overload in the recipient fetus....
PHOTODYNAMIC THERAPY IN THE TREATMENT OF LARGE DUODENAL PAPILLA AND EXTRAHEPATIC BILE DUCTS CANCER
Authors: E Stranadko, A Lobakov, V Morokhotov, M Riabov, V Duvansky
Journal: Endoscopy (2019)

Cancers of Vater's papilla and extrahepatic bile ducts are hardly accessible tumor localizations characterized by extensive tumor growth and poor prognosis. Surgical resection provides limited success with rather high recurrence rate. Photodynamic therapy (PDT) is a new technique, providing both, adequate tumor destruction and minimal damage to surrounding tissue.
BREATH ANALYSIS CAN BE USED TO DETECT COLORECTAL CANCER: PROOF-OF-PRINCIPLE STUDY
Authors: KE van Keulen, ME Jansen, RWM Schrauwen, JJ Kolkman, PD Siersema
Journal: Endoscopy (2019)

Colorectal cancer (CRC) is the third most common cancer diagnosis and a major cause of mortality worldwide. This study aims to assess if breath analysis with an electronic nose (Aeonose, the eNose company, the Netherlands) can be used as a non-invasive screening test for CRC.
Is Echocardiography Critical in Patients With Kawasaki Disease With a Z-Score &amp;lt; 2 6 Weeks From Onset?—Reply
Authors: Sarah D. de Ferranti, Jane W. Newburger
Journal: JAMA Pediatrics (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Knowledge, Motivations, and Practices Regarding Indoor Tanning Among Men Who Have Sex With Men in the San Francisco Bay Area
Authors: Lily Morrison, Henry F. Raymond, Kenneth A. Katz, Julia Simard, Natnaelle Admassu, Niko Pascua, Eleni Linos
Journal: JAMA Dermatology (2019)

This study assesses knowledge, motivations, and practices regarding indoor tanning among men who have sex with men in the San Francisco Bay Area using data from the National HIV Behavioral Surveillance survey.
EUS-GUIDED RADIOFREQUENCY ABLATION PLUS CHEMOTHERAPY VERSUS CHEMOTHERAPY ALONE FOR UNRESECTABLE PANCREATIC CANCER (ERAP): PRELIMINARY RESULTS OF A PROSPECTIVE COMPARATIVE STUDY
Authors: K Tiankanon, P Kongkam, T Orprayoon, T Luangsukrerk, DW Seo, V Sriuranpong, C Nantavithya, T Jantarattana, A Cañones, P Angsuwatcharakon, W Ridtitid, P Kullavanijaya, R Rerknimitr
Journal: Endoscopy (2019)

To compare EUS-RFA plus chemotherapy versus chemotherapy (CMT) alone as a primary treatment of unresectable pancreatic cancer (UPC) in a prospective comparative cohort study.
Release of paused RNA polymerase II at specific loci favors DNA double-strand-break formation and promotes cancer translocations
Authors: Gaetano Ivan Dellino, Fernando Palluzzi, Andrea Maria Chiariello, Rossana Piccioni, Simona Bianco, Laura Furia, Giulia De Conti, Britta A. M. Bouwman, Giorgio Melloni, Davide Guido, Luciano Giacò, Lucilla Luzi, Davide Cittaro, Mario Faretta, Mario Nicodemi, Nicola Crosetto, Pier Giuseppe Pelicci
Journal: Nature Genetics (2019)
Prognostic value of Ki-67 in patients with triple-negative breast cancer receiving neo-adjuvant or adjuvant chemotherapy: A systematic review and meta-analysis
Authors: Q. Zhou, W. Li
Journal: Annals of Oncology (2019)

Background: Ki-67 is a widely used marker of tumor proliferation, but the prognostic value of Ki-67 in triple-negative breast cancer (TNBC) is still controversial. The aim of present meta-analysis was conducted to evaluate the association between Ki-67 expression and survival in TNBC patients receiving neo-adjuvant or adjuvant chemotherapy. Methods: Relevant literatures were identified from PubMed, Embase, Web of Science, China National Knowledge Infrastructure, and Cochrane Library, up to March 14, 2018. Hazard ratio (HR) with 95% confidence intervals (CI) were calculated as effect values, and heterogeneity, sensitivity analysis and publication bias analyses were also performed. Results: 35 studies enrolling 7716 patients were eligible for inclusion. Data on overall survival (OS) and disease-free survival (DFS) were available from 25 and 29 studies, respectively. The pooled results showed that high Ki-67 expression was significantly associated with poor DFS (HR: 1.73, 95% CI: 1.45–2.07, p < 0.001) and OS (HR: 1.65, 95% CI: 1.27–2.14, p < 0.001) compared with low Ki-67 expression in TNBC. Similar results were observed on subgroup analysis by Ki-67 or ER/PR cut-off value, treatment strategies, or districts. Conclusions: High Ki-67 was a poor prognostic factor for TNBC who received neo-adjuvant or adjuvant chemotherapy. Legal entity responsible for the study: Wen Li. Funding: Has not received any funding. Disclosure: All authors have declared no conflicts of interest.
Association Between Parental Medical Claims for Opioid Prescriptions and Risk of Suicide Attempt by Their Children
Authors: David A. Brent, Kwan Hur, Robert D. Gibbons
Journal: JAMA Psychiatry (2019)

The rate of youth suicide has increased over the past 15 years in the United States as has the rate of death due to opioid overdose in adults of parental age.To explore the possible connection between parental use of prescription opioids and the increasing rate of youth suicide.A pharmacoepidemiologic study was conducted from January 1, 2010, to December 31, 2016, linking medical claims for parental opioid prescriptions with medical claims for suicide attempts by their children. The study used MarketScan medical claims data covering more than 150 million privately insured people in the United States. The study included 121 306 propensity score-matched 30- to 50-year-old parents who used opioids and parents who did not use opioids and their 10- to 19-year-old children (148 395 children of parents who did not use opioids and 184 142 children of parents who used opioids). Propensity score matching was used to identify relevant control families based on demographic features and concomitant use of psychotropic medication.Opioid use in a parent was defined as having prescription fills covering more than 365 days of an opioid between 2010 and 2016.Suicide attempt rate in the children of parents who used opioids and those who did not use opioids.A total of 148 395 children (75 575 sons and 72 820 daughters; mean [SD] age, 11.5 [1.6] years at the start of follow-up) had parents who did not use opioids and 184 142 children (94 502 sons and 89 640 daughters; mean [SD] age, 11.8 [1.8] years at the start of follow-up) with parents who did use opioids. There were 100 899 children aged 10 to 14 years and 47 496 children aged 15 to 19 years with parents who did not use opioids and 96 975 children aged 10 to 14 years and 87 163 children aged 15 to 19 years with parents who did use opioids. Of the children with parents who did not use opioids, 212 (0.14%) attempted suicide; of the children with parents who did use opioids, 678 (0.37%) attempted suicide. Parental use of opioids was associated with a doubling of the risk of a suicide attempt by their offspring (odds ratio [OR], 1.99; 95% CI, 1.71-2.33). The association remained significant after adjusting for child age and sex (OR, 1.85; 95% CI, 1.58-2.17), addition of child and parental depression and diagnoses of substance use disorder (OR, 1.46; 95% CI, 1.24-1.72), and addition of parental history of suicide attempt (OR, 1.45; 95% CI, 1.23-1.71). Geographical variation in opioid use did not change the association (OR, 2.00; 95% CI, 1.71-2.34).Children of parents who use prescription opioids are at increased risk for suicide attempts, which could be a contributing factor to the time trend in adolescent suicidality. The care of families with a parent who uses opioids should include mental health screening of their children.
Abstract 11038: Late Gadolinium Enhancement Cardiac Magnetic Resonance in Detection of Cardiac Involvement and its Prognostic Implication in Patients with Extracardiac Sarcoidosis
Authors: Toshiyuki Nagai, Shun Kohsaka, Shigeo Okuda, Toshihisa Anzai, Koichiro Asano, Keiichi Fukuda
Journal: Circulation (2012)

PURPOSE: In patients with sarcoidosis, sudden cardiac death is a leading cause of mortality, and unrecognized cardiac involvement (CI) is often noted. Recent advances in late gadolinium enhancement...