Liver injury in COVID-19: management and challenges
Authors: Chao Zhang, Lei Shi, Fu-Sheng Wang
Journal: The Lancet. Gastroenterology & hepatology (2020)

In December, 2019, an outbreak of a novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2], previously 2019-nCoV) started in Wuhan, China, and has since become a global threat to human health. The number of confirmed cases of 2019 coronavirus disease (COVID-19) has reached 87 137 worldwide as of March 1, 2020, according to WHO COVID-19 situation report 41; most of these patients are in Wuhan, China. Many cases of COVID-19 are acute and resolve quickly, but the disease can also be fatal, with a mortality rate of around 3%.1Guan W-J Ni Z-Y Hu Y et al.Clinical characteristics of 2019 novel coronavirus infection in China.N Engl J Med. 2020; (published online Feb 28.)DOI:10.1056/NEJMoa2002032Crossref Scopus (20278) Google Scholar Onset of severe disease can result in death due to massive alveolar damage and progressive respiratory failure.2Wang F-S Zhang C What to do next to control the 2019-nCoV epidemic?.Lancet. 2020; 395: 391-393Summary Full Text Full Text PDF PubMed Scopus (180) Google Scholar SARS-CoV-2 shares 82% genome sequence similarity to SARS-CoV and 50% genome sequence homology to Middle East respiratory syndrome coronavirus (MERS-CoV)—all three coronaviruses are known to cause severe respiratory symptoms. Liver impairment has been reported in up to 60% of patients with SARS3Chau TN Lee KC Yao H et al.SARS-associated viral hepatitis caused by a novel coronavirus: report of three cases.Hepatology. 2004; 39: 302-310Crossref PubMed Scopus (326) Google Scholar and has also been reported in patients infected with MERS-CoV.4Alsaad KO Hajeer AH Al Balwi M et al.Histopathology of Middle East respiratory syndrome coronovirus (MERS-CoV) infection—clinicopathological and ultrastructural study.Histopathology. 2018; 72: 516-524Crossref PubMed Scopus (228) Google Scholar At least seven relatively large-scale case studies have reported the clinical features of patients with COVID-19.1Guan W-J Ni Z-Y Hu Y et al.Clinical characteristics of 2019 novel coronavirus infection in China.N Engl J Med. 2020; (published online Feb 28.)DOI:10.1056/NEJMoa2002032Crossref Scopus (20278) Google Scholar, 5Huang C Wang Y Li X et al.Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.Lancet. 2020; 395: 497-506Summary Full Text Full Text PDF PubMed Scopus (32861) Google Scholar, 6Chen N Zhou M Dong X et al.Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study.Lancet. 2020; 395: 507-513Summary Full Text Full Text PDF PubMed Scopus (14363) Google Scholar, 7Wang D Hu B Hu C et al.Clinical characteristics of 138 hospitalised patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China.JAMA. 2020; (published online Feb 7.)DOI:10.1001/jama.2020.1585Crossref Scopus (16201) Google Scholar, 8Shi H Han X Jiang N et al.Radiological findings from 81 patients with COVID-19 pneumonia in Wuhan, China: a descriptive study.Lancet Infect Dis. 2020; (published online Feb 24.)DOI:10.1016/S1473-3099(20)30086-4Summary Full Text Full Text PDF Scopus (2575) Google Scholar, 9Xu X-W Wu X-X Jiang X-G et al.Clinical findings in a group of patients infected with the 2019 novel coronavirus (SARS-Cov-2) outside of Wuhan, China: retrospective case series.BMJ. 2020; (published online Feb 19.)DOI:10.1136/bmj.m606Crossref PubMed Scopus (1449) Google Scholar, 10Yang X Yu Y Xu J et al.Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study.Lancet Respir Med. 2020; (published online Feb 24.)DOI:10.1016/S2213-2600(20)30079-5Summary Full Text Full Text PDF Scopus (6966) Google Scholar In this Comment, we assess how the liver is affected using the available case studies and data from The Fifth Medical Center of PLS General Hospital, Beijing, China. These data indicate that 2–11% of patients with COVID-19 had liver comorbidities and 14–53% cases reported abnormal levels of alanine aminotransferase and aspartate aminotransferase (AST) during disease progression (table). Patients with severe COVID-19 seem to have higher rates of liver dysfunction. In a study in The Lancet by Huang and colleagues,5Huang C Wang Y Li X et al.Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.Lancet. 2020; 395: 497-506Summary Full Text Full Text PDF PubMed Scopus (32861) Google Scholar elevation of AST was observed in eight (62%) of 13 patients in the intensive care unit (ICU) compared with seven (25%) of 28 patients who did not require care in the ICU. Moreover, in a large cohort including 1099 patients from 552 hospitals in 31 provinces or provincial municipalities, more severe patients with disease had abnormal liver aminotransferase levels than did non-severe patients with disease.1Guan W-J Ni Z-Y Hu Y et al.Clinical characteristics of 2019 novel coronavirus infection in China.N Engl J Med. 2020; (published online Feb 28.)DOI:10.1056/NEJMoa2002032Crossref Scopus (20278) Google Scholar Furthermore, in another study,8Shi H Han X Jiang N et al.Radiological findings from 81 patients with COVID-19 pneumonia in Wuhan, China: a descriptive study.Lancet Infect Dis. 2020; (published online Feb 24.)DOI:10.1016/S1473-3099(20)30086-4Summary Full Text Full Text PDF Scopus (2575) Google Scholar patients who had a diagnosis of COVID-19 confirmed by CT scan while in the subclinical phase (ie, before symptom onset) had significantly lower incidence of AST abnormality than did patients diagnosed after the onset of symptoms. Therefore, liver injury is more prevalent in severe cases than in mild cases of COVID-19.TableComorbidity with liver disease and liver dysfunction in patients with SARS-CoV-2 infectionPatients with SARS-CoV-2 infectionPatients with pre-existing liver conditionsPatients with abnormal liver functionNotesGuan et al1Guan W-J Ni Z-Y Hu Y et al.Clinical characteristics of 2019 novel coronavirus infection in China.N Engl J Med. 2020; (published online Feb 28.)DOI:10.1056/NEJMoa2002032Crossref Scopus (20278) Google Scholar109923 (2·3%)AST abnormal (22·2%), ALT abnormal (21·3%)Elevated levels of AST were observed in 112 (18·2%) of 615 patients with non-severe disease and 56 (39·4%) of 142 patients with severe disease. Elevated levels of ALT were observed in 120 (19·8%) of patients with non-severe disease and 38 (28·1%) of 135 patients with severe disease.Huang et al5Huang C Wang Y Li X et al.Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China.Lancet. 2020; 395: 497-506Summary Full Text Full Text PDF PubMed Scopus (32861) Google Scholar411 (2·0%)15 (31·0%)Patients with severe disease had increased incidence of abnormal liver function. Elevation of AST level was observed in eight (62%) of 13 patients in the ICU compared with seven (25%) 25 patients who did not require care in the ICU.Chen et al6Chen N Zhou M Dong X et al.Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study.Lancet. 2020; 395: 507-513Summary Full Text Full Text PDF PubMed Scopus (14363) Google Scholar99NA43 (43·0%)One patient with severe liver function damage.Wang et al7Wang D Hu B Hu C et al.Clinical characteristics of 138 hospitalised patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China.JAMA. 2020; (published online Feb 7.)DOI:10.1001/jama.2020.1585Crossref Scopus (16201) Google Scholar1384 (2·9%)NA..Shi et al8Shi H Han X Jiang N et al.Radiological findings from 81 patients with COVID-19 pneumonia in Wuhan, China: a descriptive study.Lancet Infect Dis. 2020; (published online Feb 24.)DOI:10.1016/S1473-3099(20)30086-4Summary Full Text Full Text PDF Scopus (2575) Google Scholar817 (8·6%)43 (53·1%)Patients who had a diagnosis of COVID-19 confirmed by CT scan while in the subclinical phase had significantly lower incidence of AST abnormality than did patients diagnosed after the onset of symptoms.Xu et al9Xu X-W Wu X-X Jiang X-G et al.Clinical findings in a group of patients infected with the 2019 novel coronavirus (SARS-Cov-2) outside of Wuhan, China: retrospective case series.BMJ. 2020; (published online Feb 19.)DOI:10.1136/bmj.m606Crossref PubMed Scopus (1449) Google Scholar627 (11·0%)10 (16·1%)..Yang et al10Yang X Yu Y Xu J et al.Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study.Lancet Respir Med. 2020; (published online Feb 24.)DOI:10.1016/S2213-2600(20)30079-5Summary Full Text Full Text PDF Scopus (6966) Google Scholar52NA15 (29·0%)No difference for the incidences of abnormal liver function between survivors (30%) and non-survivors (28%).Our data (unpublished)562 (3·6%)16 (28·6%)One fatal case, with evaluated liver injury.13Xu Z Shi L Wang Y et al.Pathological findings of COVID-19 associated with acute respiratory distress syndrome.Lancet Respir Med. 2020; (published online Feb 18.)DOI:10.1016/S2213-2600(20)30076-XSummary Full Text Full Text PDF Scopus (6346) Google ScholarAST= aspartate aminotransferase. ALT= alanine aminotransferase. ICU=intensive care unit. Open table in a new tab AST= aspartate aminotransferase. ALT= alanine aminotransferase. ICU=intensive care unit. Liver damage in patients with coronavirus infections might be directly caused by the viral infection of liver cells. Approximately 2–10% of patients with COVID-19 present with diarrhoea, and SARS-CoV-2 RNA has been detected in stool and blood samples.11Yeo C Kaushal S Yeo D Enteric involvement of coronaviruses: is faecal–oral transmission of SARS-CoV-2 possible?.Lancet Gastroenterol Hepatol. 2020; (published online Feb 19.)DOI:10.1016/S2468-1253(20)30048-0Summary Full Text Full Text PDF PubMed Scopus (623) Google Scholar This evidence implicates the possibility of viral exposure in the liver. Both SARS-CoV-2 and SARS-CoV bind to the angiotensin-converting enzyme 2 (ACE2) receptor to enter the target cell,7Wang D Hu B Hu C et al.Clinical characteristics of 138 hospitalised patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China.JAMA. 2020; (published online Feb 7.)DOI:10.1001/jama.2020.1585Crossref Scopus (16201) Google Scholar where the virus replicates and subsequently infects other cells in the upper respiratory tract and lung tissue; patients then begin to have clinical symptoms and manifestations. Pathological studies in patients with SARS confirmed the presence of the virus in liver tissue, although the viral titre was relatively low because viral inclusions were not observed.3Chau TN Lee KC Yao H et al.SARS-associated viral hepatitis caused by a novel coronavirus: report of three cases.Hepatology. 2004; 39: 302-310Crossref PubMed Scopus (326) Google Scholar In patients with MERS, viral particles were not detectable in liver tissue.4Alsaad KO Hajeer AH Al Balwi M et al.Histopathology of Middle East respiratory syndrome coronovirus (MERS-CoV) infection—clinicopathological and ultrastructural study.Histopathology. 2018; 72: 516-524Crossref PubMed Scopus (228) Google Scholar Gamma-glutamyl transferase (GGT), a diagnostic biomarker for cholangiocyte injury, has not been reported in the existing COVID-19 case studies; we found that it was elevated in 30 (54%) of 56 patients with COVID-19 during hospitalisation in our centre (unpublished). We also found that elevated alkaline phosphatase levels were observed in one (1·8%) of 56 patients with COVID-19 during hospitalisation. A preliminary study (albeit not peer-reviewed) suggested that ACE2 receptor expression is enriched in cholangiocytes,12Chai X Hu L Zhang Y et al.Specific ACE2 expression in cholangiocytes may cause liver damage after 2019-nCoV infection.bioRxiv. 2020; (published online Feb 4.) (preprint).https://doi.org/10.1101/2020.02.03.931766Google Scholar indicating that SARS-CoV-2 might directly bind to ACE2-positive cholangiocytes to dysregulate liver function. Nevertheless, pathological analysis of liver tissue from a patient who died from COVID-19 showed that viral inclusions were not observed in the liver.13Xu Z Shi L Wang Y et al.Pathological findings of COVID-19 associated with acute respiratory distress syndrome.Lancet Respir Med. 2020; (published online Feb 18.)DOI:10.1016/S2213-2600(20)30076-XSummary Full Text Full Text PDF Scopus (6346) Google Scholar It is also possible that the liver impairment is due to drug hepatotoxicity, which might explain the large variation observed across the different cohorts. In addition, immune-mediated inflammation, such as cytokine storm and pneumonia-associated hypoxia, might also contribute to liver injury or even develop into liver failure in patients with COVID-19 who are critically ill. Liver damage in mild cases of COVID-19 is often transient and can return to normal without any special treatment. However, when severe liver damage occurs, liver protective drugs have usually been given to such patients in our unit. Chronic liver disease represents a major disease burden globally. Liver diseases including chronic viral hepatitis, non-alcoholic fatty liver disease, and alcohol-related liver disease affect approximately 300 million people in China. Given this high burden, how different underlying liver conditions influence liver injury in patients with COVID-19 needs to be meticulously evaluated. However, the exact cause of pre-existing liver conditions has not been outlined in the case studies of COVID-19 and the interaction between existing liver disease and COVID-19 has not been studied. Immune dysfunction—including lymphopenia, decreases of CD4+ T-cell levels, and abnormal cytokine levels (including cytokine storm)—is a common feature in cases of COVID-19 and might be a critical factor associated with disease severity and mortality. For patients with chronic hepatitis B in immunotolerant phases or with viral suppression under long-term treatment with nucleos(t)ide analogues, evidence of persistent liver injury and active viral replication after co-infection with SARS-CoV-2 need to be further investigated. In patients with COVID-19 with autoimmune hepatitis, the effects of administration of glucocorticoids on disease prognosis is unclear. Given the expression of the ACE2 receptor in cholangiocytes, whether infection with SARS-CoV-2 aggravates cholestasis in patients with primary biliary cholangitis, or leads to an increase in alkaline phosphatase and GGT, also needs to be monitored. Moreover, patients with COVID-19 with liver cirrhosis or liver cancer might be more susceptible to SARS-CoV-2 infection because of their systemic immunocompromised status. The severity, mortality, and incidence of complications in these patients, including secondary infection, hepatic encephalopathy, upper gastrointestinal bleeding, and liver failure, need to be examined in large-cohort clinical studies. Considering their immunocompromised status, more intensive surveillance or individually tailored therapeutic approaches is needed for severe patients with COVID-19 with pre-existing conditions such as advanced liver disease, especially in older patients with other comorbidities. Further research should focus on the causes of liver injury in COVID-19 and the effect of existing liver-related comorbidities on treatment and outcome of COVID-19. We declare no competing interests. Download .pdf (.56 MB) Help with pdf files Chinese translation of the full text