tbuckley/cabot-search huggingface dataset:
Analysis of the correlation between the expression of VEGF and αvβ5 and the ratio of eukaryotic initiation factor 4E (eif4E)/phosphorylated 4E-binding protein 1 (4EBP-1) level in hematopoietic derived cell lines.
Authors: Michael Lawson, Ellen Friday, Jonathon Glass, Francesco Turturro
Journal: Cancer Research (2004)

3235  It has been recently shown that VEGF is part of an autocrine loop that regulates migration of prostate cancer cells in bone by activating ανβ5 and ανβ3 integrins. The secretion of VEGF has been associated with the expression of eukaryotic initiation factor 4E (eIF4E), that favors the translation of 5’-UTR highly structured mRNAs, such as VEGF mRNA in several malignancies. In this study, we studied the association between the secretion of VEGF and ανβ integrins in cells derived from hematological malignancies with high bone tropism such as acute myeloid leukemia (AML), ALK+ anaplastic large cell lymphoma (ALCL) and multiple myeloma (MM). The level of secretion of VEGF165 measured by ELISA was significantly correlated (R 0.93) with the expression of ανβ5 as measured by flow cytometry in all cells except in MM-derived ARH77 cells. An increased level of surface ανβ5 (88.08 %) was present in this cell line despite low level of VEGF165 secretion (1.49 fold vs control). On the contrary, only HL60 cells (AML) presented combined elevated levels of both ανβ5 (69.16 %) and ανβ3 (45 %) associated with increased level of VEGF165 secretion (16.87 fold vs control). We identified two populations of cells based on the expression of total eIF4E as measured by densitometric analysis of Western blots. AML and MM-derived cells presented higher levels of eIF4E compared to ALCL-derived cell lines. Surprisingly, MM-derived cells ARH77 and HsSultan showed very low levels of VEGF165 (1.49 and 0.95 fold vs control, respectively) despite elevated levels of eIF4E secretion. The initiation of an efficient cap-dependent translation is related to the ratio of eIF4E to phosphorylated 4E binding protein 1 (4EBP1) that plays an important role in this process. This ratio was inversely related to the level of VEGF165 in MM and AML-derived cells but not in ALCL-derived cells. In conclusion, our data illustrate the complexity of the association of VEGF165 secretion, eIF4E-regulated expression of VEGF and the expression of ανβ5. Further investigation may help in elucidating the complexity of these mechanisms and their role in regulating the migration of hemotopoietic-derived cells in bone and other tissues.
At the Heart of the Matter
Authors: Norrisa Haynes, Lisa A. Cooper, Michelle A. Albert
Journal: Circulation (2020)

On behalf of the Association of Black CardiologistsT he coronavirus disease 2019 (COVID-19) pandemic has unmasked longstanding racial and ethnic health-related disparities.As noted by Ta-Nehisi Coates in reference to racial and ethnic inequities, the "sociology, the history, the economics, the graphs, the charts, the regressions all land with great violence on the body," 1 and the effect of COVID-19 on the body, especially on bodies of color, is too often fatal.
Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms
Authors: Emmelien Vancaester, Thomas Depuydt, Cristina Maria Osuna-Cruz, Klaas Vandepoele
Journal: Molecular Biology and Evolution (2020)

Abstract Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% of worldwide oxygen production, which can rapidly respond to favorable conditions and often outcompete other phytoplankton. We investigated the contribution of horizontal gene transfer (HGT) to its ecological success. A large-scale phylogeny-based prokaryotic HGT detection procedure across nine sequenced diatoms showed that 3–5% of their proteome has a horizontal origin and a large influx occurred at the ancestor of diatoms. More than 90% of HGT genes are expressed, and species-specific HGT genes in Phaeodactylum tricornutum undergo strong purifying selection. Genes derived from HGT are implicated in several processes including environmental sensing and expand the metabolic toolbox. Cobalamin (vitamin B12) is an essential cofactor for roughly half of the diatoms and is only produced by bacteria. Five consecutive genes involved in the final synthesis of the cobalamin biosynthetic pathway, which could function as scavenging and repair genes, were detected as HGT. The full suite of these genes was detected in the cold-adapted diatom Fragilariopsis cylindrus. This might give diatoms originating from the Southern Ocean, a region typically depleted in cobalamin, a competitive advantage. Overall, we show that HGT is a prevalent mechanism that is actively used in diatoms to expand its adaptive capabilities.
Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency
Authors: In Young Choi, HoTae Lim, Alex Huynh, James Schofield, Hyeon Jin Cho, Hosuk Lee, Peter Andersen, Joo Heon Shin, Won Do Heo, Sang-Hwan Hyun, Yong Jun Kim, Yohan Oh, Hyesoo Kim, Gabsang Lee
Journal: Biomaterials (2020)
Feature Tracking Myocardial Strain Incrementally Improves Prognostication in Myocarditis Beyond Traditional CMR Imaging Features
Authors: Kady Fischer, Sarah J. Obrist, Sophie A. Erne, Anselm W. Stark, Maximilian Marggraf, Kyoichi Kaneko, Dominik P. Guensch, Adrian T. Huber, Simon Greulich, Ayaz Aghayev, Michael Steigner, Ron Blankstein, Raymond Y. Kwong, Christoph Gräni
Journal: JACC. Cardiovascular imaging (2020)
Abstract 14321: Dabigatran-Associated Bleedings May Result From Pharmacokinetic Interactions With Mild P-gp Inhibitors in Very Elderly Patients
Authors: Marjorie Bernier, Nadege Parassol, Thibaud Lavrut, Milou-Daniel Drici
Journal: Circulation (2019)

Introduction: AFib shall concern more than half of the very elderly population by 2050. The benefit risk ratio of anticoagulants must thereforebe optimized in this population. Direct oral anticoagu...
CONTRASTING EFFECTS OF VERAPAMIL AND NIFEDIPINE ON PH OF ISCHEMIC MYOCARDIUM DURING FIXED CORONARY-OCCLUSION
Authors: Ro Ym, Patterson Re, Markle D, Mcguire D, Goldstein S, Speir E, Greene R, Aamodt R, Epstein Se
Journal: Journal of the American College of Cardiology (1983)
Bringing NHS data analysis into the 21st century
Authors: Ben Goldacre, Martin Bardsley, Tim Benson, Kate Cheema, Roger Chinn, Ellen Coughlan, Sarah Dougan, Marc Farr, Loraine Hawkins, Adrian Jonas, Andy Kinnear, Morag Mcinnes, Mohammed Amin Mohammed, Caroline Morton, Rahul Pasumarthy, Neil Pettinger, Ben Rowland, Neil Sebire, Paul Stroner, Jeni Tennison, Samantha Warnakula, Oliver Watson, Emma Wright, Hamish Young, Jessica Morley
Journal: Journal of the Royal Society of Medicine (2020)
A tertiary center experience of multiple myeloma patients with COVID-19: lessons learned and the path forward
Authors: Bo Wang, Oliver Van Oekelen, Tarek H. Mouhieddine, Diane Marie Del Valle, Joshua Richter, Hearn Jay Cho, Shambavi Richard, Ajai Chari, Sacha Gnjatic, Miriam Merad, Sundar Jagannath, Samir Parekh, Deepu Madduri
Journal: Journal of Hematology & Oncology (2020)

Abstract Background The COVID-19 pandemic, caused by SARS-CoV-2 virus, has resulted in over 100,000 deaths in the USA. Our institution has treated over 2000 COVID-19 patients during the pandemic in New York City. The pandemic directly impacted cancer patients and the organization of cancer care. Mount Sinai Hospital has a large and diverse multiple myeloma (MM) population. Herein, we report the characteristics of COVID-19 infection and serological response in MM patients in a large tertiary care institution in New York. Methods We performed a retrospective study on a cohort of 58 patients with a plasma-cell disorder (54 MM, 4 smoldering MM) who developed COVID-19 between March 1, 2020, and April 30, 2020. We report epidemiological, clinical, and laboratory characteristics including the persistence of viral detection by polymerase chain reaction (PCR) and anti-SARS-CoV-2 antibody testing, treatments initiated, and outcomes. Results Of the 58 patients diagnosed with COVID-19, 36 were hospitalized and 22 were managed at home. The median age was 67 years; 52% of patients were male and 63% were non-White. Hypertension (64%), hyperlipidemia (62%), obesity (37%), diabetes mellitus (28%), chronic kidney disease (24%), and lung disease (21%) were the most common comorbidities. In the total cohort, 14 patients (24%) died. Older age (&gt; 70 years), male sex, cardiovascular risk, and patients not in complete remission (CR) or stringent CR were significantly ( p &lt; 0.05) associated with hospitalization. Among hospitalized patients, laboratory findings demonstrated elevation of traditional inflammatory markers (CRP, ferritin, D-dimer) and a significant ( p &lt; 0.05) association between elevated inflammatory markers, severe hypogammaglobulinemia, non-White race, and mortality. Ninety-six percent (22/23) of patients developed antibodies to SARS-CoV-2 at a median of 32 days after initial diagnosis. The median time to PCR negativity was 43 (range 19–68) days from initial positive PCR. Conclusions Drug exposure and MM disease status at the time of contracting COVID-19 had no bearing on mortality. Mounting a severe inflammatory response to SARS-CoV-2 and severe hypogammaglobulinemia was associated with higher mortality. The majority of patients mounted an antibody response to SARS-CoV-2. These findings pave a path to the identification of vulnerable MM patients who need early intervention to improve outcomes in future outbreaks of COVID-19.
T‐cell receptor repertoire analysis for the diagnosis and treatment of solid tumor: A methodology and clinical applications
Authors: Na Li, Jiani Yuan, Wenjia Tian, Lin Meng, Yongyu Liu
Journal: Cancer Communications (2020)

Abstract T cells, which are involved in adaptive immunity, are essential in the elimination of tumor cells. Mature T cells can specifically recognize the antigen on the major histocompatibility complex (MHC) molecule through T‐cell receptors (TCR). The unique rearrangement mechanisms during T‐cell maturation provide great diversity to TCR, ensuring specific recognition between T cells and antigens. Thus, TCR repertoire analysis occupied an important position in T‐cell regarding research. Nowadays, next‐generation sequencing technology allows the simultaneous detection of TCR sequences with high throughput, and several evaluation indexes facilitate the measure of TCR repertoire. Based on this new methodology, discoveries are made across a range of tumor types. Results have shed light on the TCR repertoire differences between cancer patients and healthy control as well as between individual's lesions, paracancer, and peripheral blood samples. The potential of TCR repertoire as a biomarker for immunotherapy efficacy is also widely studied as TCR repertoire represents different baseline within individuals and shows dynamic change during treatment. Accurate delineation of the T‐cell repertoire can further the understanding of the immune system response to tumorigenesis. Still, existing researches are insufficient to clarify the specific clinical implications of TCR dynamic change and the definite role of TCR repertoire diversity during the treatment process. The results of some studies are even contrary. In this article, we reviewed TCR rearrangement mechanisms and analysis methods. Recent progress of TCR sequencing technology in tumor research is also discussed. In conclusion, intensive studies over an extended range of cancer types and a broadened group of subjects should be carried to solidify the TCR repertoire's position as an immunotherapy biomarker.
Reply to Lipworth <i>et al.</i>: Inhaled Corticosteroids and COVID-19
Authors: Tania Maes, Ken Bracke, Guy G. Brusselle
Journal: American Journal of Respiratory and Critical Care Medicine (2020)

Maes and colleagues (1) fail to point out the inhibitory in vitro effects of ICS on local and systemic production of 6), this being the strongest predictor for impending respiratory failure in severe coronavirus disease (COVID-19) (7).Finally, a more specific suppressive effect from ICS on SARS-CoV-2 replication has been described with ciclesonide and mometasone furoate but not with budesonide, beclomethasone, or fluticasone (8).We believe that, taken together, these observations reinforce the need for patients with eosinophilic asthma and COPD to continue receiving their controller therapy containing ICS, as that will provide optimal disease control and perhaps also confer protection against viral triggers, perhaps including SARS-CoV-2.
A Pooled Analysis of Diffusion-Weighted Imaging Lesions in Patients With Acute Intracerebral Hemorrhage
Authors: Santosh B. Murthy, Sung-Min Cho, Ajay Gupta, Ashkan Shoamanesh, Babak B. Navi, Radhika Avadhani, Joshua Gruber, Yunke Li, Tatiana Greige, Vasileios-Arsenios Lioutas, Casey Norton, Cenai Zhang, Pitchaiah Mandava, Costantino Iadecola, Guido J. Falcone, Kevin N. Sheth, Alessandro Biffi, Jonathan Rosand, Adnan I. Qureshi, Joshua N. Goldstein, Chelsea Kidwell, Issam Awad, Magdy Selim, Daniel F. Hanley, Daniel Woo, Hooman Kamel, Wendy C. Ziai
Journal: JAMA Neurology (2020)

The etiology and significance of diffusion-weighted imaging (DWI) lesions in patients with acute intracerebral hemorrhage (ICH) remain unclear.
Developing Primary Care–Based Recommendations for Social Determinants of Health: Methods of the U.S. Preventive Services Task Force
Authors: Karina W. Davidson, Alex R. Kemper, Chyke A. Doubeni, Chien-Wen Tseng, Melissa A. Simon, Martha Kubik, Susan J. Curry, Justin Mills, Alex Krist, Quyen Ngo-Metzger, Amanda Borsky
Journal: Annals of Internal Medicine (2020)

The purpose of the U.S. Preventive Services Task Force (USPSTF) is to provide evidence-based recommendations on primary care screening, behavioral counseling, and preventive medications. A person's health is strongly influenced by social determinants of health, such as economic and social conditions; therefore, preventive recommendations that address these determinants would be ideal. However, differing social determinants have been proposed by a wide range of agencies and organizations, little prevention evidence is available, and responsible parties are in competition, all of which make the creation of evidence-based prevention recommendations for social determinants of health challenging. This article highlights social determinants already included in USPSTF recommendations and proposes a process by which others may be considered for primary care preventive recommendations. In many ways, incorporating social determinants of health into evidence-based recommendations is an evolving area. By reviewing the evidence on the effects of screening and interventions on social determinants relevant to primary care, the USPSTF will continue to provide recommendations on clinical preventive services to improve the health of all Americans.
Radiation-free removal of common bile duct stones in pregnancy facilitated by endoscopic ultrasound and single-operator cholangioscopy
Authors: Chalapathi Rao Achanta
Journal: Endoscopy (2020)

A 26-year-old woman who was 6 months pregnant presented with a 5-day history of jaundice, right upper abdominal discomfort, and pruritus. Abdominal ultrasound showed gallstones and a dilated common bile duct (CBD) with a suspicious calculous in the lower CBD. Serum bilirubin was 2.9 mg/dL and serum alkaline phosphatase was 216 IU. A radiation-free procedure to remove choledocholithiasis was planned under propofol sedation, and informed consent was obtained.
SAT0101 Analysis of the efficacy of cyclosporin a in 56 psoriatic patients
Authors: I Krajnc, K Lukanoviè, I Holc
Journal: Annals of the Rheumatic Diseases (2001)

<h3>Background</h3> <h3>Objectives</h3> 20% of the patients with psoriatic arthritis (PA) may develop a very severe form of arthritis. When the skin disease is extensive, it makes sense to use medications which are thought to be effective for both, the skin and joint disease. The efficacy of the cyclosporin A (CyA) on the skin lesiones in psoriasis has been shown in several controlled studies. A few authors demonstrated the anti-inflammatory efficacy of the drug in the PA. The most important action of CyA is the inhibition of production of cytokines by T-cells. We have evaluated efficacy and tolerability of CyA in PA patients.56 patients with average age of 47 (range 33–71), 21 female and 35 male have been treated with 3 mg/kg per day for 6 months. 19 patients received low doses of corticosteroids, nobody received other DMARD during the treatment. <h3>Methods</h3> Several clinical and laboratory parameters for the assessment of joint status at the start and at the end of the study were employed: Ritchie index, duration in minutes of morning stiffness with a semi-quantitive evaluation the presence or absence of the onychopathy. We also performed at monthly interval the systolic blood pressure values and the laboratory parameters: erythrocyte sedimentation rate, CRP, serum creatinine and blood urea and liver enzymes. We measured a concentration of the drug in serum with Fluorescence polarisation immuno assay (ABOTT) method at the beginning and at the end of the study. <h3>Results</h3> The results are shown in the following Table 1. (statistical-analysis t-student-test) <h3>Conclusion</h3> Six of our patients drop out because of high blood pressure, all other patients finished the study. Our results show a good tolerability and efficacy on the joint pain, on the morning stiffness and on the skin lesions.
TReND in Africa: Toward a Truly Global (Neuro)science Community
Authors: Tom Baden, Mahmoud Bukar Maina, Andre Maia Chagas, Yunusa Garba Mohammed, Thomas O. Auer, Ana Silbering, Lukas von Tobel, Marie Pertin, Renee Hartig, Jelena Aleksic, Ibukun Akinrinade, Mosab A. Awadelkareem, Artemis Koumoundourou, Aled Jones, Fabiana Arieti, Andrew Beale, Daniel Münch, Samyra Cury Salek, Sadiq Yusuf, Lucia L. Prieto-Godino
Journal: Neuron (2020)
Ventricular tachyarrhythmia detection by implantable loop recording in patients with heart failure and preserved ejection fraction: the <scp>VIP‐HF</scp> study
Authors: Dirk J. van Veldhuisen, Gijs van Woerden, Thomas M. Gorter, Vanessa P.M. van Empel, Olivier C. Manintveld, Robert G. Tieleman, Alexander H. Maass, Kevin Vernooy, B. Daan Westenbrink, Isabelle C. van Gelder, Michiel Rienstra
Journal: European Journal of Heart Failure (2020)

The primary aim of the VIP-HF study was to examine the incidence of sustained ventricular tachyarrhythmias (VTs) in heart failure (HF) with mid-range (HFmrEF) or preserved ejection fraction (HFpEF). Secondary aims were to examine the incidence of non-sustained VTs, bradyarrhythmias, HF hospitalizations and mortality.
Inhibition of growth and metastasis of breast carcinoma by tranilast
Authors: Chakrabarti Rabindranath, Serg Jothy, Gerald Prudhomme
Journal: Cancer Research (2008)

1176  Tranilast (N-[3, 4 - dimethoxycin-namoyl]-anthranalic acid) is used clinically in Japan as an anti-allergic agent and to control fibrotic disorders. The anti-fibrotic effect of tranilast is thought to be mediated through the inhibition of TGF-β1 and possibly other mediators. Tranilast has also been shown to inhibit the growth of many tumor cells, such as glioma cells, uterine leiomyoma cells, oral squamous cell carcinoma and pancreatic tumor. It is well tolerated and has few adverse effects as compared to conventional anticancer drugs. In the present study, we explored the anti-tumor effect of tranilast in experimental mammary tumor, as the metastasis of breast cancer very much depends on TGF-β1. BALB/c mice were inoculated with syngenic mammary tumor cells (4T1) in mammary fat pads and tranilast was given daily by gavage. Tumor volumes were measured at various time points. After 4 weeks, mice were sacrificed and various tissues were fixed for routine histology. Our results showed that tranilast markedly reduced (> 50 %) the growth of the primary tumor in vivo. The drug also drastically reduced (> 90 %) metastases to the lungs and liver. In addition, tranilast suppressed the growth of 4T1 cells in a dose-dependent manner in culture, without any cytotoxic effect. Growth of human MDA-MB-231 breast tumor cells and other cancer cells were also inhibited by tranilast in culture. Tranilast inhibited the epithelial to mesenchymal transdifferentiation of 4T1, which is important for metastasis. Thus, these results showed that the tranilast has potential to be an effective therapeutic agent in breast cancer.
NRF2 is a key regulator of endothelial microRNA expression under proatherogenic stimuli
Authors: Suvi Linna-Kuosmanen, Vanesa Tomas Bosch, Pierre R Moreau, Maria Bouvy-Liivrand, Henri Niskanen, Emilia Kansanen, Annukka Kivelä, Juha Hartikainen, Mikko Hippeläinen, Hannu Kokki, Pasi Tavi, Anna-Liisa Levonen, Minna U Kaikkonen
Journal: Cardiovascular Research (2020)

Oxidized phospholipids and microRNAs (miRNAs) are increasingly recognized to play a role in endothelial dysfunction driving atherosclerosis. NRF2 transcription factor is one of the key mediators of the effects of oxidized phospholipids, but the gene regulatory mechanisms underlying the process remain obscure. Here, we investigated the genome-wide effects of oxidized phospholipids on transcriptional gene regulation in human umbilical vein endothelial cells and aortic endothelial cells with a special focus on miRNAs.We integrated data from HiC, ChIP-seq, ATAC-seq, GRO-seq, miRNA-seq, and RNA-seq to provide deeper understanding of the transcriptional mechanisms driven by NRF2 in response to oxidized phospholipids. We demonstrate that presence of NRF2 motif and its binding is more prominent in the vicinity of up-regulated transcripts and transcriptional initiation represents the most likely mechanism of action. We further identified NRF2 as a novel regulator of over 100 endothelial pri-miRNAs. Among these, we characterize two hub miRNAs miR-21-5p and miR-100-5p and demonstrate their opposing roles on mTOR, VEGFA, HIF1A, and MYC expressions. Finally, we provide evidence that the levels of miR-21-5p and miR-100-5p in exosomes are increased upon senescence and exhibit a trend to correlate with the severity of coronary artery disease.Altogether, our analysis provides an integrative view into the regulation of transcription and miRNA function that could mediate the proatherogenic effects of oxidized phospholipids in endothelial cells.
Abstract 12389: Should Paramedics Withhold Nitroglycerin in Patients With Prehospital Chest Pain Who Are Tachycardiac?
Authors: Mohammad Alhamaydeh, Ziad Faramand, Christian Martin-Gill, Clifton Callaway, Salah Al-Zaiti
Journal: Circulation (2018)

Background: Sublingual nitroglycerin (NTG) is frequently used in the prehospital setting in patients with suspected acute myocardial infarction. Current AHA/ACC guidelines recommends against admini...
223Ra-Dichloride Response Evaluation Using 18F-Fluciclovine PET/CT and Bone Scintigraphy in a Patient With Castration-Resistant Metastatic Prostate Cancer
Authors: Jiaqiong Wang, Russ Kuker, Aldo Serafini
Journal: Clinical Nuclear Medicine (2020)

Abstract A 66-year-old man was diagnosed with metastatic prostate cancer to the bones. The patient started 223 Ra-dichloride (Xofigo) therapy in April 2019. 99m Tc-MDP bone scan and 18 F-fluciclovine (Axumin) PET/CT showed discordant but overall complementary findings that indicated disease progression after 5 doses of Xofigo therapy. The patient’s prostate-specific antigen increased from 33.81 ng/mL at baseline before Xofigo therapy and up to 394.3 ng/mL after the fifth dose of Xofigo treatment. Because of disease progression, Xofigo therapy was discontinued.