tbuckley/cabot-search huggingface dataset:
Stability of Conversion Factors for BCR-ABL Monitoring -– Implications for the Frequency of Validation Rounds
Authors: Martin C. Müller, Umang Munjal, Philipp Erben, Thomas Lion, Gerlinde Mitterbauer-Hohendanner, Hakim El Housni, Nancy Boeckx, Renata Zadro, Jiri Mayer, Peter Rohon, Jana Rulcova, Lykke Grubach, Kadri Raudsepp, Veli Kairisto, Francois-Xavier Mahon, Claude Preudhomme, Sandrine Hayette, Jean Gabert, Jean-Michel Cayuela, Christian Thiede, Lars Galonska, Heike Pfeifer, Carsten Hirt, Philippe Schafhausen, Nils von Neuhoff, Martin Roskos, Christiane Pott, Thoralf Lange, Georg Hess, Susanne Schnittger, Annika Dufour, Steffen Koschmieder, Frank Stegelmann, Katerina Zoi, Kostas Stamatopoulos, Hajnalka Andrikovics, Tali Tohami, Giovanni Martinelli, Barbara Izzo, Enrico Gottardi, Mindaugas Stoskus, Dag Andre Nymoen, Tomasz Sacha, Joana Diamond, Rodica Talmaci, Andrey Misyurin, Michael V. Dubina, Tadej Pajic, Josep F. Nomdedeu, Dolors Colomer, Gisela Barbany-Bustinza, Hans Ehrencrona, Elisabeth Oppliger Leibundgut, Jeroen Janssen, Vincent H. J. van der elden, Peter J.M. Valk, Ugur Ozbek, Lihui Wang, Gareth Gerrard, Helen E White, Thomas Schenk, Thomas Ernst, Rüdiger Hehlmann, Giuseppe Saglio, Andreas Hochhaus, Nicholas C. P. Cross
Journal: Blood (2010)
A Dominant Mutation in Mitochondrial Unfoldase CLPX Results in Erythropoietic Protoporphyria
Authors: Yvette Y Yien, Gael Nicolas, Lisa van der Vorm, Laurent Gouya, Hector Begonia, Martin Kafina, Jean-Charles Deybach, Tania Baker, Julia Kardon, John D. Phillips, Hervé Puy, Barry H. Paw
Journal: Blood (2016)
Cortactin Is a New Player in Aggressiveness and Diffusion of Chronic Lymphocytic Leukaemia
Authors: Veronica Martini, Federica Frezzato, Valentina Trimarco, Marco Pizzi, Giorgia Chiodin, Filippo Severin, Edoardo Scomazzon, Vincenza Guzzardo, Deborah Saraggi, Flavia Raggi, Leonardo Martinello, Monica Facco, Andrea Visentin, Gianpietro Semenzato, Livio Trentin
Journal: Blood (2016)
The Effect of a No-Prophylactic Versus Prophylactic Platelet Transfusion Strategy On Bleeding in Patients with Hematological Malignancies and Severe Thrombocytopenia (TOPPS trial). A Randomized Controlled, Non-Inferiority Trial
Authors: Simon J. Stanworth, Lise Estcourt, Gillian Powter, Brennan C. Kahan, Claire Dyer, Lekha Bakrania, Charlotte Llewelyn, Louise Choo, Linley Bielby, Derek Norfolk, Erica M. Wood, Michael F. Murphy
Journal: Blood (2012)
Induced Resistance to Bortezomib in Preclinical Model of Waldenstrom Macroglobulinemia Is Associated with Bcl-2 Upregulation.
Authors: Kasyapa Chitta, Jean-Gabriel Coignet, Sakina Sojar, Pushpankur Ghoshal, Kiersten M. Miles, Leighton Stein, Morton Coleman, Richard R. Furman, Craig Hofmeister, Suzanne Hayman, Kelvin P. Lee, Asher A. Chanan-Khan
Journal: Blood (2009)
The Ubiquitous Chromatin Opening Element (UCOE) Enhances Lentiviral Cytidine Deaminase (CDD) Expression and Drug Resistance During Hematopoietic Differentiation of Murine Induced Pluripotent Stem Cells (iPSCs),
Authors: Nils Pfaff, Nico Lachmann, Mania Ackermann, Saskia Kohlscheen, Christian Brendel, Michael Flasshove, Axel Schambach, Thomas Moritz
Journal: Blood (2011)
Objective Measures of Pre-Transplant Physiologic Fitness Are Strong Predictors of Very-Short Term Transplantation Related Mortality
Authors: Joshua A Fein, Roni Shouval, Noga Shem-Tov, Ivetta Danylesko, Ronit Yerushalmi, Vered Dulberg, Maya Muchnick, Shalom Hassid, Ramit Mehr, Avichai Shimoni, Arnon Nagler
Journal: Blood (2016)
Markers for Increased Thrombotic Risk within the Complete Blood Count
Authors: Sæmundur Rögnvaldsson, Sigrún H. Lund, Malin Hultcrantz, Signý Vala Sveinsdóttir, Guðný Eiríksdóttir, Tamara B Harris, Vilmundur Guðnason, Sigurdur Y Kristinsson
Journal: Blood (2016)
Evidence Relating Health Care Provider Burnout and Quality of Care
Authors: Daniel S. Tawfik, Annette Scheid, Jochen Profit, Tait Shanafelt, Mickey Trockel, Kathryn C. Adair, J. Bryan Sexton, John P.A. Ioannidis
Journal: Annals of Internal Medicine (2019)

Whether health care provider burnout contributes to lower quality of patient care is unclear.To estimate the overall relationship between burnout and quality of care and to evaluate whether published studies provide exaggerated estimates of this relationship.MEDLINE, PsycINFO, Health and Psychosocial Instruments (EBSCO), Mental Measurements Yearbook (EBSCO), EMBASE (Elsevier), and Web of Science (Clarivate Analytics), with no language restrictions, from inception through 28 May 2019.Peer-reviewed publications, in any language, quantifying health care provider burnout in relation to quality of patient care.2 reviewers independently selected studies, extracted measures of association of burnout and quality of care, and assessed potential bias by using the Ioannidis (excess significance) and Egger (small-study effect) tests.A total of 11 703 citations were identified, from which 123 publications with 142 study populations encompassing 241 553 health care providers were selected. Quality-of-care outcomes were grouped into 5 categories: best practices (n = 14), communication (n = 5), medical errors (n = 32), patient outcomes (n = 17), and quality and safety (n = 74). Relations between burnout and quality of care were highly heterogeneous (I2 = 93.4% to 98.8%). Of 114 unique burnout-quality combinations, 58 indicated burnout related to poor-quality care, 6 indicated burnout related to high-quality care, and 50 showed no significant effect. Excess significance was apparent (73% of studies observed vs. 62% predicted to have statistically significant results; P = 0.011). This indicator of potential bias was most prominent for the least-rigorous quality measures of best practices and quality and safety.Studies were primarily observational; neither causality nor directionality could be determined.Burnout in health care professionals frequently is associated with poor-quality care in the published literature. The true effect size may be smaller than reported. Future studies should prespecify outcomes to reduce the risk for exaggerated effect size estimates.Stanford Maternal and Child Health Research Institute.
High Efficacy and Significantly Shortened Neutropenia Of Dose-Dense S-HAM As Compared To Standard Double Induction: First Results Of a Prospective Randomized Trial (AML-CG 2008)
Authors: Jan Braess, Karl-Anton Kreuzer, Karsten Spiekermann, Hans Walter Lindemann, Eva Lengfelder, Ullrich Graeven, Peter Staib, Wolf-Dieter Ludwig, Harald Biersack, Yon-Dschun Ko, Michael J. Uppenkamp, Stefan Korsten, Maike De Wit, Rudolf Peceny, Tobias Gaska, Xaver Schiel, Dirk M Behringer, Michael G. Kiehl, Bettina Zinngrebe, Gerald Meckenstock, Eva Roemer, Dirk Medgenberg, Ernst Spaeth-Schwalbe, Birgit Braess, Gero Massenkeil, Heidrun Hindahl, Guido Trenn, Rainer Schwerdtfeger, Susanne Amler, Cristina Sauerland, Andreas Faldum, Michael Unterhalt, Stefan K. Bohlander, Stephanie Schneider, Annika Dufour, Christian Buske, Michael Fiegl, Marion Subklewe, Anja Baumgartner, Bernhard J. Woermann, Dietrich Beelen, Wolfgang Hiddemann
Journal: Blood (2013)
Bone Marrow Biopsy for the Staging of Diffuse Large B-Cell Lymphoma: Is It Necessary Bilateral Trephine Biopsy?.
Authors: Marcelo Bellesso, Rodrigo D. Velasques, Luis F. Pracchia, Beatriz Beitler, Vera L. Aldred, Dalton Chamone, Jose M. Kutner, Juliana Pereira
Journal: Blood (2007)
Advancing functional connectivity research from association to causation
Authors: Andrew T. Reid, Drew B. Headley, Ravi D. Mill, Ruben Sanchez-Romero, Lucina Q. Uddin, Daniele Marinazzo, Daniel J. Lurie, Pedro A. Valdés-Sosa, Stephen José Hanson, Bharat B. Biswal, Vince Calhoun, Russell A. Poldrack, Michael W. Cole
Journal: Nature Neuroscience (2019)
MicroRNA Profiling in Benign and Malignant Conjunctival Melanocytic Lesions
Authors: Jolique A. van Ipenburg, Ad J.M. Gillis, Ing, Lambert C.J. Dorssers, Quincy C.C. van den Bosch, Rita van Ginderdeuren, Guy S. Missotten, Nicole Naus, Dion Paridaens, Leendert H.J. Looijenga, Robert M. Verdijk
Journal: Ophthalmology (2019)
The replication-competent HIV-1 latent reservoir is primarily established near the time of therapy initiation
Authors: Melissa-Rose Abrahams, Sarah B. Joseph, Nigel Garrett, Lynn Tyers, Matthew Moeser, Nancie Archin, Olivia D. Council, David Matten, Shuntai Zhou, Deelan Doolabh, Colin Anthony, Nilu Goonetilleke, Salim Abdool Karim, David M. Margolis, Sergei Kosakovsky Pond, Carolyn Williamson, Ronald Swanstrom
Journal: Science Translational Medicine (2019)

Although antiretroviral therapy (ART) is highly effective at suppressing HIV-1 replication, the virus persists as a latent reservoir in resting CD4+ T cells during therapy. This reservoir forms even when ART is initiated early after infection, but the dynamics of its formation are largely unknown. The viral reservoirs of individuals who initiate ART during chronic infection are generally larger and genetically more diverse than those of individuals who initiate therapy during acute infection, consistent with the hypothesis that the reservoir is formed continuously throughout untreated infection. To determine when viruses enter the latent reservoir, we compared sequences of replication-competent viruses from resting peripheral CD4+ T cells from nine HIV-positive women on therapy to viral sequences circulating in blood collected longitudinally before therapy. We found that, on average, 71% of the unique viruses induced from the post-therapy latent reservoir were most genetically similar to viruses replicating just before ART initiation. This proportion is far greater than would be expected if the reservoir formed continuously and was always long lived. We conclude that ART alters the host environment in a way that allows the formation or stabilization of most of the long-lived latent HIV-1 reservoir, which points to new strategies targeted at limiting the formation of the reservoir around the time of therapy initiation.
Mechanism of Regulating Fas Signaling by Promyelocytic Leukemia Protein (PML) and Its Dominant-Negative Mutant PMLRARα
Authors: Rong-Hua Tao, Zuzana Berkova, Jillian F Wise, Haifeng Zhu, Xue Ao, Zeming Chen, Wenzhuo Zhuang, Frank K Braun, Rohit Mathur, Felipe Samaniego
Journal: Blood (2012)
Major Improvement of Invasive Aspergillosis Outcome Is Not Enough to Improve Overall Survival In Allogeneic Hematopoietic Stem Cell Transplant Recipients: Results From a Single-Center Retrospective Analysis
Authors: Géraldine Salmeron, Raphaël Porcher, Anne Bergeron, Marie Robin, Regis Peffault de Latour, Vanderson Rocha, Anna D. Petropoulou, Alienor Xhaard, Annie Sulahian, Gérard Socié, Patricia Ribaud
Journal: Blood (2010)
Cerebral Vein Thrombosis In Patients With Myeloproliferative Neoplasms
Authors: Ida Martinelli, Valerio De Stefano, Alessandra Carobbio, Maria Luigia Randi, Claudia Santarossa, Alessandro Rambaldi, Maria Chiara Finazzi, Francisco Cervantes, Eduardo Arellano-Rodrigo, Serena Rupoli, Lucia Canafoglia, Alessia Tieghi, Facchini Luca, Silvia Betti, Alessandro M Vannucchi, Lisa Pieri, Rossella Cacciola, Emma Cacciola, Agostino Cortelezzi, Alessandra Iurlo, Enrico Maria Pogliani, Elena Maria Elli, Antonio Spadea, Tiziano Barbui
Journal: Blood (2013)
Clinical Outcome of 27 Imatinib Mesylate Resistant Chronic Myelogenous Leukemia (CML) Patients Harbouring a T315I BCR-ABL Mutation.
Authors: Franck E. Nicolini, Emmanuel Bachy, Sélim Corm, Quoc-Hung Lê, Sandrine Hayette, Dominique Bories, Michel Tulliez, Nathalie Sorel, Francois Guilhot, Laurence Legros, François-Xavier Mahon, Frédéric Maloisel, Jean-Jacques Kiladjian, Catherine Roche-Lestienne, Claude Preudhomme, Mauricette Michallet
Journal: Blood (2006)
Impact of K-ras status on FOLFOX and XELOX regimens efficacy in metastatic colorectal (mCRC) patients (pts).
Authors: Mikhail Fedyanin, Alexey Tryakin, Ilya Pokataev, Igor Bazin, Vechaslav Aliev, Sergey Gordeev, August Garin, Sergei Tjulandin
Journal: Journal of Clinical Oncology (2013)

e14674 Background: Predictive value of K-ras status for the treatment with anti-EGFR monoclonal antibodies is well established. There is a lack of data about predictive significance of K-ras status for oxaliplatin-based chemotherapy (CT). We retrospectively studied prognostic and predictive significance of mutant K-ras in mCRC pts treated with FOLFOX or XELOX in 1 st line CT. Methods: We analyzed data on 127 pts with mCRC, who were treated in our department during 2008-2011 with oxaliplatin-based regimens. K-ras status was determined by PCR analysis in 51/127 (40,1%) pts. Mutant K-ras was detected in 16/51 (31,3%) pts: 13/16 (81,3%) – codon 12, 3/16 (18,7%) – codon 13. mFOLFOX6 was administered in 21/51 (41,2%), XELOX – in 30/51 (58,8%) pts. No pts received anti-EGFR mAb in the 1-st line. Median follow-up was 9 months (range, 2 - 41). The mean number of courses was 9 and 5 in FOLFOX and XELOX groups, respectively. PFS was chosen as a primary end-point. Results: Median PFS was 7,5 months for all 127 pts, 8,4 months in pts with FOLFOX and 7,5 months in pts with XELOX. Median PFS in pts with wild type K-ras was 10,3 months and 6,5 months in pts with mutant K-ras (p=0,1, HR 1,7, 95%CI 0,9-3,4). Negative prognostic significance of mutant K-ras was seen in XELOX group (PFS, 10,9 vs 5,7 months, р= 0,002) but not in FOLFOX group (PFS, 7,7 vs 9,3 months, р= 0,48). In pts with wild type K-ras XELOX lead to longer PFS than FOLFOX regimen - 10,9 vs 7,5 months, respectively (р=0,09, HR 0,52, 95%CI 0,23-1,14). And vice versa, in pts with mutant K-ras FOLFOX was more active than XELOX – median PFS was 9,3 vs 5,7 months, respectively (р=0,009, HR 0,3, 95%CI 0,05-0,64). Conclusions: Our findings probably suggest that K-ras status influences the efficacy of FOLFOX and XELOX regimens in mCRC pts. These data need to be confirmed in larger series of pts.
Application of MS score in macrophage activation syndrome patients associated with adult onset Still’s disease
Authors: Ran Wang, Ting Li, Shuang Ye, Wenefng Tan, Cheng Zhao, Yisha Li, Chunde Bao, Qiong Fu
Journal: Annals of the Rheumatic Diseases (2019)

We read with great interest the article by Minoia et al which named development and initial validation of the macrophage activation syndrome (MAS)/systemic juvenile idiopathic arthritis (sJIA) (MS) score for diagnosis of MAS in sJIA.

MAS is a life-threatening complication of rheumatic disorders, including sJIA, adult-onset Still’s disease (AOSD) and lupus.1–4 Timely diagnosis and appropriate treatment of MAS are particularly important to improve the prognosis of MAS patients. At present, hemophagocytic lymphohistiocytosis (HLH)-2004 and HLH-2009 criteria are widely used to identify MAS associated with AOSD. Hemophagocytic syndrome diagnostic (HS) score was developed previously to facilitate MAS recognition, but still requires validation.5 In 2019, Francesca Minoia et al reported a MS score for classification of sJIA-associated MAS patients.6 Considering that sJIA and AOSD are thought to constitute the …
Recipient and Donor KIRs Ligands Match Prevents CMV Reactivation Post-Haploidentical T Cell-Replete Transplantation
Authors: Xiang-Yu Zhao, Xue-Yi Luo, Xing-Xing Yu, Xiaosu Zhao, Meng Lv, Ting-Ting Han, Ying-Jun Chang, Ming-Rui Huo, Lan-Ping Xu, Xiao-Hui Zhang, Kai-Yan Liu, Dan Li, Xiao-Jun Huang
Journal: Blood (2016)
Countermeasures for urgent visit of patients treated with immune checkpoint inhibitors in Nagoya Memorial Hospital
Authors: Satoshi Hibi, Yuko Shirokawa, Kengo Nanya, Yuko Tomomatsu, Satoshi Kayukawa, Takashi Yosida, Kentaro Hirade, Megumi Kabeya, Takae Kataoka, Shu Yuasa, Kenji Ina
Journal: Annals of Oncology (2019)
Epigenetic Priming Using Decitabine Followed By Cytarabine As an Induction Regimen in Older Patients with Newly Diagnosed Acute Myeloid Leukemia
Authors: Annie Im, Jing-Zhou Hou, Anastasios Raptis, Mounzer Agha, Rafic Farah, Robert L. Redner, Alison R. Sehgal, Kathleen A. Dorritie, Ann Welsh, Daniel E. Johnson, Daniel Normolle, Michael Boyiadzis
Journal: Blood (2014)
Risk of Venous Thromboembolism Recurrence Among Rivaroxaban Treated Patients Who Continued Versus Discontinued Therapy: Analyses Among VTE Patients
Authors: Alok A. Khorana, Jeffrey S Berger, Philip S Wells, Roger Seheult, Veronica Ashton, François Laliberté, Concetta Crivera, Dominique Lejeune, Jeff Schein, Peter Wildgoose, Patrick Lefebvre, Scott Kaatz
Journal: Blood (2016)
Genome-Wide Analysis of Microdeletions and Identification of NF1 Inactivation in Acute Myelogenous Leukemia.
Authors: Sami Malek, Peter Ouillette, Yin Wang, Yan Liu, Whitney Wright, Diane Roulston, Amanda L Dressel, Judith Karp, Paula Bockenstedt, Ammar Al-Zoubi, Moshe Talpaz, Lisa Kujawski, Yang Liu, Kerby Shedden, Sajid Shakhan, Cheng Li, Harry Paul Erba, Brian Parkin
Journal: Blood (2009)
Association of anti-Ro52 autoantibodies with interstitial lung disease in connective tissue diseases
Authors: Si Wu, Xiaojun Tang, Liping Wu, Liang-jing Lu, Xuebing Feng
Journal: Annals of the Rheumatic Diseases (2019)

We read with great interest the article by Sabbagh et al demonstrating that anti-Ro52 autoantibodies were connected with the development of interstitial lung disease (ILD) in patients with juvenile myositis.1 Anti-Ro52 has been found in a variety of connective tissue diseases (CTDs) and drawn considerable attention from rheumatologists in recent years. Besides myositis, these autoantibodies have also been reported to be related to ILD in several other CTDs, but with great discrepancy across the studies.2 In addition, it remains unclear how the incidence of ILD differs in the presence of anti-Ro52 alone or in combination with anti-Ro60 (Sjogren's syndrome related antigen A), one of the most associated antibodies that may determine anti-Ro52 …
The Extent of Labile Plasma Iron (LPI) Predicts for Non-Relapse-Mortality (NRM) in AML and MDS Patients with Systemic Iron Overload Undergoing Allogenic Stem Cell Transplantation Results of the Prospective, German-Austrian Allive Trial
Authors: Martin Wermke, Julia Eckoldt, Katharina Goetze, Stefan A. Klein, Gesine Bug, Friedrich Stölzel, Malte von Bonin, Johannes Schetelig, Michael Laniado, Verena Plodeck, Wolf-Karsten Hofmann, Gerhard Ehninger, Martin Bornhäuser, Dominik Wolf, Igor Theurl, Uwe Platzbecker
Journal: Blood (2015)
Micro RNAs in Acute Myeloid Leukemia
Authors: Aakriti Pandita, Alina Basnet, Poornima Ramadas, Aarati Poudel, Ankit Anand, Nibal Saad, Syed A. Akbar, Salman I. Chaudhry, Frank Middleton, Diana M. Gilligan
Journal: Blood (2016)
Synoviorthesis (Radionuclide Synovectomy) with Yttrium -90 for Chronic Synovitis in Haemophilia : A Cost-Effective Therapy in Developing Countries.
Authors: Ajay Sharma, Velu Nair, Satyaranjan Das, PG Kumar, Deepak Kumar Mishra, Jasjit Singh
Journal: Blood (2009)
VE Cadherin Positive Endothelial Cells Regulate Hematopoietic Reconstitution In Vivo.
Authors: J. Lauren Russell, Phuong Doan, Heather A Himburg, Sarah K. Meadows, Pamela Daher, Katherine Helms, Mamle Quarmyne, Nelson J. Chao, David Kirsch, John P. Chute
Journal: Blood (2010)
PCR-Based Liposomal Amphotericin B Treatment Following Allogeneic Stem Cell Transplantation Is a Safe Treatment Strategy: Preliminary Results of a Prospective Study.
Authors: Holger Hebart, L. Klingspor, Thomas Klingebiehl, Juergen Loeffler, J. Tollemar, Per Ljungman, Hannes Wandt, H.J. Dornbusch, Ulrike Schumacher, Christoph Meisner, C. Engel, Lothar Kanz, Ole Ringden, Hermann Einsele
Journal: Blood (2004)
Utility of Next Generation Sequencing in Prognostication and Therapeutic Decision Making in Cytogenetically Normal AML with DNMT3A Mutations
Authors: Arjun Datt Law, Mahadeo A. Sukhai, Mariam Thomas, Andrea Arruda, Narmin Ibrahimova, Steven M. Chan, Vikas Gupta, Mark D. Minden, Aaron D. Schimmer, Hassan Sibai, Karen W.L. Yee, Dwayne L. Barber, Tracy L. Stockley, Suzanne Kamel-Reid, Andre C. Schuh
Journal: Blood (2016)
Condensed Versus Standard Schedule of High-Dose Cytarabine Consolidation Therapy with Pegfilgrastim Growth Factor Support in Acute Myeloid Leukemia
Authors: Sonia Jaramillo, Axel Benner, Jurgen Krauter, Hans Martin, Thomas Kindler, Martin Bentz, Helmut R. Salih, Gerhard Held, Caus-Henning Köhne, Katharina Götze, Michael Lübbert, Andrea Kündgen, Peter Brossart, Mohammad Amen Wattad, Hans-Juergen Salwender, Bernd Hertenstein, David Nachbaur, Gerald Wulf, Heinz A. Horst, Heinz Kirchen, Walter Fiedler, Aruna Raghavachar, Gudrun Russ, Stephan Kremers, Elisabeth Koller, Volker Runde, Gerhard Heil, Daniela Weber, Brigitte Schlegelberger, Konstanze Döhner, Arnold Ganser, Hartmut Döhner, Richard F. Schlenk
Journal: Blood (2016)
Clinical Relevance of CD45+/CD19- Stem-like Tumor Cells in Patients with Mantle Cell Lymphoma: A Single Center Experience
Authors: Sung Min Kim, Seung-Tae Lee, Kyung Ju Ryu, Hee-Jin Kim, Sun-Hee Kim, Kwai Han Yoo, Young Hyeh Ko, Won Seog Kim, Seok Jin Kim
Journal: Blood (2014)
Non-viral delivery of therapeutic microRNAs to glioma cells using chitosan nanocomplexes
Authors: Marjorie Boissinot, Sarra Limam, Maria Collado-González, Yadira Gonzalez-Espinosa, Heiko Wurdak, Francisco M Goycoolea, Susan C Short
Journal: Neuro-Oncology (2019)

Abstract One of the biggest challenges when treating brain tumours is achieving efficient delivery of therapeutic agents to the brain and more specifically to the cancer cells. MicroRNA-1300 was identified in our group as a very promising therapeutic microRNA given its cytotoxic effect when introduced in both established as well as cancer-stem-like patient-derived glioblastoma cultures, while not affecting differentiated glioblastoma cells. We are now collaborating to assess the potential efficiency of the natural biopolymer chitosan to form nanocomplexes containing the mature form of microRNA-1300 for delivery. Chitosan has been established as a highly attractive biocompatible polymer to deliver both in vitro and in vivo therapeutic nucleotides intracellularly. In previous studies, we have shown chitosan’s efficacy to form spherical nanocomplexes with microRNA and apply them to the downregulation of JAMA-A mRNA in MCF-7 breast cancer cells. Chitosan can also be chemically conjugated to introduce affinity towards a wide range of cellular targets (e.g. with aptamers). Methods We have optimised of the composition and characterised the biophysical properties of chitosan-microRNA nanocomplexes of varying (+/-) charge ratios using both a control nontargeting microRNA coupled to a fluorochrome (CS-miRdy547, efficiency of cell entry) and mature microRNA-1300 (CS-mi1300, efficient release and biological effect). We have tested the nanocomplexes in 2D monolayers and 3D spheroid cultures on established U251 as well as two patient-derived cultures. Reverse transfection was used as positive control. Results The control nanoparticles of CS-miRdy547 are taken up by the patient-derived cultures in 2D and 3D. Analysis is ongoing using the CS-miR-1300 nanoparticles.
Sequence of Therapy in Multiple Myeloma: Does It Matter? Retrospective Evaluation of Patients with Multiple Myeloma Who Have Received Bortezomib Followed by Lenalidomide or Vice Versa,
Authors: Amila M Patel, Viet Q. Ho, Kenneth H. Shain, Daniel Sullivan, Melissa Alsina, Leonel Ochoa, Taiga Nishihori, Benjamin Djulbegovic, William Dalton, Rachid Baz
Journal: Blood (2011)
Abstract 18759: Vascular Smooth Muscle Cells Derived From Inbred Swine Induced Pluripotent Stem Cells for Vascular Tissue Engineering
Authors: Yibing Qyang, Jiesi Luo, Lingfeng Qin, Mehmet Kural, Darrel Kotton, Robert Hawley, David H Sachs, Laura Niklason
Journal: Circulation (2016)

Introduction: Development of autologous tissue-engineered vascular constructs using vascular smooth muscle cells (VSMCs) derived from human induced pluripotent stem cells (hiPSCs) holds great poten...
Gain-of-Function Mutations and Copy Number Increases of Notch2 in Diffuse Large B-Cell Lymphoma.
Authors: Suk Young Lee, Keiki Kumano, Kumi Nakazaki, Masashi Sanada, Go Yamamoto, Yasuhito Nannya, Ritsuro Suzuki, Satoshi Ota, Yasunori Ota, Koji Izutsu, Mamiko Sakata-Yanagimoto, Akira Hangaishi, Hideo Yagita, Masashi Fukayama, Masao Seto, Mineo Kurokawa, Seishi Ogawa, Shigeru Chiba
Journal: Blood (2007)