tbuckley/cabot-search huggingface dataset:
Nodular lymphocyte-predominant and classical Hodgkin lymphoma subtypes: Differences in biology, survival, and impact of radiotherapy.
Authors: Shihab Ali, Adam J. Olszewski
Journal: Journal of Clinical Oncology (2013)

8550 Background: Hodgkin lymphoma (HL) is a heterogeneous disease, but differences between nodular lymphocyte predominant (NLPHL) and classical (CHL) subtypes were previously studied in small cohorts preventing adjustment for confounders. We studied those differences based on the Surveillance, Epidemiology and End Results (SEER) program data. Methods: We analyzed SEER HL cases aged 16 years and over, diagnosed between 1995 and 2009. We studied the following endpoints: crude probability of HL-related death (HLRD), relative survival (using individual data in multivariate flexible parametric models) and risk of secondary malignancies (using competing risk regression). We studied the impact of radiotherapy (RT) in early-stage disease using a propensity score, adjusting for treatment selection and immortal time bias. Results: We identified 25,903 patients, with disparate age, race and stage distributions between subtypes. In a multivariate model, NLPHL demonstrated significantly better crude and net survival outcomes (Table), but in contrast to all CHL subtypes, it showed a steady increase in mortality rate after 2 years. The risk of secondary non-Hodgkin lymphoma was significantly higher in both NLPHL (hazard ratio, HR, 2.28, P=0.002) and lymphocyte-rich (LR) CHL (HR 2.08, P=0.01) than in nodular sclerosis (NS). The risk of other secondary malignancies did not differ between subtypes. After balancing confounding factors in treatment arms, RT in stage I/II was associated with improved survival in NS (HR, 0.78, P=0.001) and LR-CHL (HR 0.36, P&lt;0.001), but not in NLPHL (HR 0.98, P=0.94) or in the remaining CHL subtypes. Conclusions: Studies of NLPHL and CHL subtypes should account for their disparate biology and clinical course. Prospective evaluation of NLPHL treatment strategies without RT is justified. [Table: see text]
Abstract 18915: Modulation of Exosomal Pro-angiogenic MicroRNAs from CD34+ Progenitor Cells by Hypoxic-Preconditioning Enhances its Angiogenic Potency and Post-ischemic Tissue Repair
Authors: Susmita Sahoo, Rajesh Gupta, Tina Thorne, Sol Misener, Douglas W Losordo, Douglas E Vaughan
Journal: Circulation (2012)

Exosomes, membrane bound nano-vesicles secreted by the cells, has great potential to advance cell-based therapies for ischemic cardiovascular diseases by overcoming its limitations- such as viability and retention, difficulty in isolation of adequate number of cells, or, appropriate storage of the cells. Recently, we have discovered that adult human CD34+ stem cells, one of the most successful cell types for treating cardiovascular diseases, secrete exosomes (Exo) that mediates most of the angiogenic and therapeutic activity of the stem cells via transfer of pro-angiogenic microRNAs. We hypothesize that the regenerative efficacy of the cell-free Exo derived from human CD34+ cells can be improved by hypoxic pre-conditioning, modulating its pro-angiogenic miRNA content. Methods and Results: CD34+ Exo isolated from hypoxic human CD34+ cells were significantly proliferative, anti-apoptotic and angiogenic both in vitro and in vivo, as compared to CD34+ Exo from normoxic cells. Treatment of hypoxic Exo significantly improved perfusion and prevented ischemic limb amputation, compared with normoxic Exo, in a mouse model of hind limb ischemia. We investigated the possibility of involvement of proteins by comparing the total proteins from hypoxic and normoxic Exo using 2-D differential gel electrophoresis and mass spectrometry; we did not detect any difference in the proteins expressions quantified. Interestingly, bioanalyzer analyses indicated that hypoxic cells and Exo were enriched for miRNAs. The differential expression of miRNAs between hypoxic and normoxic Exo are profiled using miRNA microarray and validated by qRT-PCR. We detected significantly high expression of proangiogenic miRNAs in hypoxic Exo. Flow cytometry and in vitro trafficking by confocal microscopy demonstrates transfer of Cy3 labeled miRNA to recipient endothelial cells. We are studying the involvement of transferred pro-angiogenic miRNAs on hypoxia-Exo-induced angiogenic activity and modulation of target endothelial cell gene expression by loss of function-gain of function studies. Conclusion: Our results demonstrate that hypoxia modulates the miRNA content of adult human CD34+ progenitor cell derived exosomes improving its angiogenic and therapeutic potency.
Abstract 12330: 30-Day Readmissions in Young Adults With ST-Elevation Myocardial Infarction: A 5-Year Nationwide Analysis
Authors: Karan Sud, Vivek Modi, Dhrubajyoti Bandyopadhyay, Eyal Herzog
Journal: Circulation (2018)

Introduction: There is an increasing incidence of myocardial infarction (MI) among younger adults due to increased prevalence of risk factors. Limited data is available regarding in-hospital outcom...
Case 18-2019: A 24-Year-Old Woman with a Pelvic Mass
Authors: David R. Spriggs, Alexander Melamed, Weier Li, Nida Safdar
Journal: New England Journal of Medicine (2019)

A 24-year-old woman was evaluated at the hospital for a pelvic mass. Several weeks earlier, intermittent vaginal bleeding, increased abdominal girth, and mild pelvic pain had developed. The blood human chorionic gonadotropin level was elevated. Management decisions were made.
FRI0067 ANXIETY AND DEPRESSION IN A PREGNANCY AND RHEUMATIC DISEASES CLINIC
Authors: Cassandra Michele Skinner Taylor, Lorena Pérez Barbosa, María Eugenia Corral Trujillo, Janett Carmen Riegatorres, Dionicio Ángel Galarza-Delgado, Jairo Vladimir Rodriguez Hinojosa
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background:</h3> Preconceptional psychologic evaluation allows the identification of risks factors for perinatal disorders. Anxiety (13.4%) and depression (41.5%) has been reported in rheumatic diseases<sup>1</sup>. Hospital Anxiety and Depression Scale (HADS) is used to evaluate anxiety and depression in patients with chronic diseases<sup>2</sup>. <h3>Objectives:</h3> To describe the frequency of anxiety and depression disorders among preconceptional patients in a Pregnancy and Rheumatic Diseases Clinic. <h3>Methods:</h3> An observational, cross-sectional, and descriptive study was conducted in the Rheumatology Consultation of Hospital Universitario "Dr. José Eleuterio González" in Monterrey, Mexico. We included 100 women aged between 18 and 39 years in the preconceptional stage of the Pregnancy and Rheumatic Diseases Clinic. HADS, which is divided in two subscales, one for anxiety and one for depression, indicating a disorder when a score ≥11 was applied. To evaluate Rheumatoid Arthritis (RA) a Disease Activity Score (DAS28) was used, for Systemic Lupus Erythematous (SLE) a Systemic Lupus Erythematous Disease Activity index (SLEDAI) was performed, and others (Sjögren's syndrome, dermatomyositis and fibromyalgia) were evaluated with the Global Quality of Life (GQL). To analyze differences among groups, Gamma coefficient was used. It was considered a statistically significant p&lt;0.05. <h3>Results:</h3> One hundred women in childbearing age were included, with a mean age of 34.48 (SD 18.33) years. RA was diagnosed in 48%, SLE in 37%, and others in 15%. We found a mean HADS total score of 11.73 (7.29), in the anxiety subscale 6.94 (4.027), and depression subscale 4.79 (4.026). A significant correlation between SLE and a high HADS score was observed (Table 1). <h3>Conclusion:</h3> In order to have a safe pregnancy, an early intervention to assess the psychiatric status is required in patients with rheumatic diseases. <h3>References:</h3> [1] Quintana, J.M., Padierna, A., Esteban, C., Arostegui, I., Bilbao, A., &amp; Ruiz, !. (2003). Evaluation of the psuchometric characteristics of the Spanish versión of the Hospital Anxiety and Depression Scale. Acta Scandinavica, 107(3), 216-221. [2] Zigmond, A. S., &amp; Snaith, R. P. (1983). The hospital Anxiety and depresión scale. Acta psychiatrica scandinavica, 67(6), 361-370. <h3>Disclosure of Interests:</h3> None declared
Abstract 16349: Revascularization After Ischemia is Partially Mediated by Clonally Expanding Endothelial Cells
Authors: Yosif Manavski, Tina Lucas, Andreas M Zeiher, Reinier A Boon, Stefanie Dimmeler
Journal: Circulation (2017)

Vascularization is critical to maintain organ function. Outgrowth and clonal expansion of endothelial cells (ECs) has been documented in vitro, however, the contribution of clonally expanding ECs t...
Prevalence of Periodontal Disease and Periodontopathic Bacteria in Anti–Cyclic Citrullinated Protein Antibody–Positive At-Risk Adults Without Arthritis
Authors: Kulveer Mankia, Zijian Cheng, Thuy Do, Laura Hunt, Josephine Meade, Jing Kang, Val Clerehugh, Alastair Speirs, Aradhna Tugnait, Elizabeth M. A. Hensor, Jackie L. Nam, Deirdre A. Devine, Paul Emery
Journal: JAMA Network Open (2019)

The prevalence of periodontitis is increased in patients with rheumatoid arthritis (RA) and periodontopathic bacteria can citrullinate proteins. Periodontitis may, therefore, be an initiator of RA and a target for prevention. Periodontal disease and periodontal bacteria have not been investigated in at-risk individuals with RA autoimmunity but no arthritis.To examine periodontal disease and periodontopathic bacteria in anti-cyclic citrullinated protein (anti-CCP) antibody-positive at-risk individuals without arthritis.This cross-sectional study took place at a teaching hospital from April 27, 2015, to May 8, 2017. Forty-eight anti-CCP-positive individuals without arthritis (CCP+ at-risk) were recruited nationally. Twenty-six patients with early RA (ERA) and 32 healthy control individuals were recruited locally. Data were analyzed between June 1, 2017, and December 1, 2017.Periodontal assessment and examination of joints using ultrasonography.Prevalence of diseased periodontal sites, clinical periodontitis, and periodontal inflamed surface area in CCP+ at-risk individuals compared with patients with ERA and healthy individuals matched for age and smoking. Paired-end sequencing of DNA from subgingival plaque from diseased and healthy periodontal sites was performed and DNA was profiled and analyzed.A total of 48 CCP+ at-risk individuals (mean [SD] age, 51.9 [11.4] years; 31 [65%] female), 26 patients with ERA (mean [SD] age, 54.4 [16.7] years; 14 [54%] female), and 32 healthy individuals (mean [SD] age, 49.4 [15.3] years; 19 [59%] female) were recruited. Of 48 CCP+ at-risk individuals, 46 had no joint inflammation on ultrasonography. Thirty-five CCP+ at-risk individuals (73%), 12 healthy individuals (38%), and 14 patients with ERA (54%) had clinical periodontitis. The median (interquartile range) percentage of periodontal sites with disease was greater in CCP+ at-risk individuals compared with healthy individuals (3.3% [0%-11.3%] vs 0% [0%-0.7%]) and similar to patients with ERA (1.1% [0%-13.1%]). Median (interquartile range) periodontal inflamed surface area was higher in CCP+ at-risk individuals compared with healthy individuals (221 mm2 [81-504 mm2] vs 40 mm2 [12-205 mm2]). Patients with CCP+ at-risk had increased relative abundance of Porphyromonas gingivalis (but not Aggregatibacter actinomycetemcomitans) at healthy periodontal sites compared with healthy individuals (effect size, 3.00; 95% CI, 1.71-4.29) and patients with ERA (effect size, 2.14; 95% CI, 0.77-3.52).This study found increased prevalence of periodontitis and P gingivalis in CCP+ at-risk individuals. This suggests periodontitis and P gingivalis are associated with disease initiation and could be targets for preventive interventions in RA.
PB1732 ASSOCIATION OF FLUDARABIN, CYTARABINE AND GEMTUZUMAB FOR FIRST‐LINE REFRACTORY AML IN CHILDREN: A SINGLE‐CENTER EXPERIENCE
Authors: M. Penel‐Page, A. Plesa, S. Girard, A. Marceau‐Renaut, C. Renard, Y. Bertrand
Journal: HemaSphere (2019)

Background: Acute myeloblastic leukemia (AML) is a rare disease in children, with only 50 to 60% of event‐free survival. Ten percents of patients do not respond to first‐line chemotherapy, and there is no recommendation about their second‐line treatment. Gemtuzumab ozogamicin (GO) is a monoclonal antibody targeted against CD33, linked to calicheamicin. Aims: We report the efficacy and tolerance of a salvage regimen with fludarabin, cytarabine and GO (FLA‐GO) in our comprehensive cancer center, for patients refractory to first‐line treatment. Methods: Eight patients (median age 14.5 years) received this protocol between 2013 and 2018. They received Gemtuzumab 3 mg/m 2 on day 1, 4, 7, associated with cytarabine 2000 mg/m 2 and fludarabin 30 mg/m 2 d1‐d5. Results: Six out of the 8 treated patients achieved complete remission with blast count ≤10 ‐2 . Five of them received a second course. We observed 11 febrile neutropenia, including 6 septicemias without complication. There was no fungal infection, nor toxic death. Two patients received G‐CSF. One had partial platelet recovery (CRp), one prolonged pancytopenia. The 8 patients received hematopoietic stem cell transplant (HSCT). We observed 5 mild to severe sinusoidal obstruction syndrome during HSCT procedure, especially in patients who did not receive prophylaxis with defibrotide. At the date of last contact, 6 patients are in continuous complete remission, with a median follow‐up of 41 months (12‐51). Two patients presented post‐HSCT relapse and died. Summary/Conclusion: FLA‐GO is a good salvage regimen for refractory AML in children, with acceptable toxicity. HSCT is mandatory to achieve sustained CR in these patients.
AB1399-HPR FOOT AND ANKLE ASSESSMENT IN PATIENTS WITH DIFFERENT GRADES OF KNEE OSTEOARTHRITIS
Authors: KARAPINAR MERVE, Ferdi Baskurt, Zeliha Baskurt, Meric Unal
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Foot and ankle profile has long been considered to contribute to the development of knee osteoarthritis (OA). It may alter the mechanical alignment and dynamic function of the lower limb especially knee functions. It is important to determined compensatory changes in the foot and ankle posture in patients with different grades of knee osteoarthritis. Because it may help in understanding the role of foot orthoses, footwear modifications or treatment strategies on alignment and function of knee <h3>Objectives</h3> This present study was designed to assess the changes in foot and ankle profile among different grades of knee OA. <h3>Methods</h3> Patients were assessed with physical examination including foot and knee specifically and anteroposterior and lateral direct radiographies. All patients were divided into four groups for medial compartment OA severity by using the Kellgren-Lawrence grading system of knee OA on weight bearing knee radiographies. The Western Ontario and Mc Master University Osteoarthritis Index (WOMAC) questionnaire was performed to assess pain and the functional status of the patients. Structural foot profile was assessed by the Foot Posture Index (FPI) system and American Orthopaedic Foot and Ankle Society (AOFAS) anklehindfoot and midfoot scales. <h3>Results</h3> The study included forty-four patients with mean age 57.1212.2 years. In terms of severity of knee OA groups; percentages for grade 0-1,2,3 and 4 were 25%,22.7%,25%,27.2% respectively. In the grade 4 and 3 with knee OA; WOMAC total score, pain and function subscale scores were higher (p &lt; 0.001). Besides, foot pronation was also increased as the severity of osteoarthritis increased. In the grade 0-1 and grade 2; AOFAS hindfoot and midfoot scores were higher (p&lt;0.001) compere to severe OA (grade 3 and grade 4). <h3>Conclusion</h3> The study indicates that differences of the severity of OA have influence on the foot and ankle profile to different degrees. Therefore, it is clinically important to give appropriate orthotics advice or treatment to patients with different degrees of OA to correct the pronation of foot to different degrees. <h3>References</h3> [1] Saito, I., Okada, K., Wakasa, M., Abe, H., &amp; Saito, A. (2018). Foot pressure pattern, hindfoot deformities, and their associations with foot pain in individuals with advanced medial knee osteoarthritis. Gait &amp; posture, 59, 83-88. <h3>Disclosure of Interests</h3> None declared
Abstract 20561: Detecting Pre-Clinical Pro-Arrhythmia Risk in vitro Using Mature Human Induced Pluripotent Stem Cell Derived Cardiomyocyte Monolayers in a Quantitative Electrophysiological High Content Screen
Authors: Katherine F Campbell, André Monteiro da Rocha, Sergey Mironov, Jiang Jiang, José Jalife, Todd J Herron
Journal: Circulation (2017)

Introduction: Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) offer a novel in vitro platform for pre-clinical cardiotoxicity and pro-arrhythmia screening of drugs in develop...
Prospective non-invasive evaluation of CXCR4 expression for the diagnosis of MALT lymphoma using [<sup>68</sup>Ga]Ga-Pentixafor-PET/MRI
Authors: Alexander R. Haug, Asha Leisser, Wolfgang Wadsak, Markus Mitterhauser, Sarah Pfaff, S. Kropf, Hans-Juergen Wester, Marcus Hacker, Markus Hartenbach, Barbara Kiesewetter-Wiederkehr, Markus Raderer, Marius E. Mayerhoefer
Journal: Theranostics (2019)

MALT lymphomas express the chemokine receptor CXCR4 on a regular basis, and [68Ga]Ga-Pentixafor-PET has been shown to quantify CXCR4 expression non-invasively. We, therefore, aimed to evaluate [68Ga]Ga-Pentixafor-PET/MRI for the non-invasive assessment of MALT lymphomas.We included 36 MALT lymphoma patients, who had not undergone previous systemic or radiation therapy, in our prospective, IRB-approved, proof-of-concept study. Involved anatomic regions were the orbit (n=14), stomach (n=10), lungs (n=5), and other sites (soft-tissues n=3; adrenal gland, tonsils, parotid gland, and urinary bladder n=1, respectively). MRI sequences included an axial 2-point Dixon T1 VIBE SPAIR 3D sequence for PET attenuation correction; a coronal T2 HASTE sequence; and an axial echo-planar imaging SPAIR-based diffusion-weighted sequence (DWI) obtained during free-breathing (b-values, 50 and 800), with corresponding ADC (apparent diffusion coefficient) maps.In 33/36 patients, there were MALT lymphomas with an increased uptake of [68Ga]Ga-Pentixafor; all current lymphoma manifestations showed an increased uptake and, accordingly, were positive on the PET/MRI. The remaining three patients had undergone surgery for their orbital MALT lymphomas prior to PET/MRI. Mean SUVmax was 8.6 ± 4.7, mean SUVmean was 4.7 ± 1.8, and mean SUVpeak was 8.0 ± 4.2. The mean SUVmax of the liver was 1.8, and the mean tumor-to-liver ratio was 2.9 ± 2.0. There were no significant differences in SUVmax (P=0.22), SUVmean (P=0.53), SUVpeak (P=0.29), or SUVt/l (P=0.92) between the four anatomic regions (orbit, stomach, lungs, other). The mean tumor volume was 146 ± 499.Our results thus indicate that [68Ga]Ga-Pentixafor-PET is feasible for the assessment of MALT lymphomas, with a good tumor-to-background ratio in terms of radiotracer uptake.
PB1813 FRONTLINE AUTOLOGOUS STEM CELL TRANSPLANTATION IN PERIPHERAL T-CELL LYMPHOMA: A SINGLE CENTER EXPERIENCE
Authors: R. El Fatmi, I. Gharbi, L. Torjemane, D. Belloumi, R. Ouerghi, N. Ben Abdejelil, A. Lakhal, S. Ladeb, T. Ben Othman
Journal: HemaSphere (2019)

Background: Peripheral T-cell lymphoma (PTCL) is a rare heterogeneous lymphoma with a poor long-term survival. Aims: Our purpose was to assess the efficacy and prognosis of autologous stem cell transplantation (ASCT) as a frontline treatment for PTCL. Methods: Patients (pts) with primary diagnosed PTCL received 6–8 weeks of pre transplant induction chemotherapy. If in complete or partial remission, they proceeded to consolidation with BEAM based ASCT. We retrospectively analyzed all consecutive patients with PTCL treated with ASCT in the Tunisian National Center of Bone Marrow transplantation from January 2004 to February 2017. PTCL diagnosis confirmed by immunohistochemistry according to 2016 WHO classification criteria. Results: In all, 32 pts were enrolled. Median age at ASCT was 41 years (range 24 to 60 years) and 23 patients (72%) were male. PTCL subtypes included ALK-negative anaplastic T cell lymphoma (TCL) (n = 12); PTCL not otherwise specified, (n = 9); angioimmunblastic TCL (n = 2); enteropathy type TCL (n = 2); extra nodal natural killer TCL, nasal type (n = 2); and hepato splenic TCL (n = 2). 81.2% had advanced disease (Ann Arbor stage III or IV). Three patients (9,4%) had bone marrow involvement and 47 % had elevated serum lactate dehydrogenase. 72 % of all patients had a good PS (WHO 0 to 1). According to IPI, pts were classified low risk (33%), low intermediate (53%) or high intermediate (13%). Pre-transplant first line chemotherapy was CHOP/CHOEP regimen (60%) or ACVBP regimen (40%). 30% of pts had refractory disease and received second line chemotherapy. Pts achieved CR (61%) or PR (39%) prior to ASCT. The median time to neutrophil engraftment was 9 days (range 7 to 16) and the median time to platelet engraftment was 11 days (range 6 to 18). The median hospital stay was 33 days (range 17 to 99). After a median follow-up post ASCT of 23 months (range 5 to 150), 12 patients remained alive. The main cause of death was lymphoma progression (73%). Non-relapse mortality was 3%. The 5-year DFS and OS rate was 35% and 38% respectively. Patients transplanted in CR had a 5-year OS rate of 42%, compared with an OS of 31% in patients in PR. Summary/Conclusion: Frontline ASCT following conventional chemotherapy in PTCL is safe and may be beneficial