tbuckley/cabot-search huggingface dataset:
COVID-19 prevalence and the impact on quality of life from stringent social distancing in a single large UK rheumatology centre
Authors: Natasha Cleaton, Sabrina Raizada, Nick Barkham, Srinivasan Venkatachalam, Tom Sheeran, Tochukwu Adizie, Hem Sapkota, Baldev Singh, James Bateman
Journal: Annals of the Rheumatic Diseases (2020)

We were interested to read the recent report from Gianfrancesco et al 1 in which they highlight impacts of medication on COVID-19 rates from an international registry. An important public health prevention measure advocated by European League Against Rheumatism (EULAR) and the British Society for Rheumatology has been stringent social isolation ‘shielding’ for patients on current immunomodulatory medication. ‘Shielding’ patients remain isolated in their homes, having minimal contact with even members of their household, for potentially 12 weeks or more.2 To complement Gianfresesco’s1 work, we present data from a large rheumatology cohort in the UK evaluating impact of treatment group and shielding on COVID-19 incidence. We also present data on impact of therapy and shielding on health-related quality of life (HRQoL).

We undertook an audit of our follow-up patient cohort (1 February 2020 to 1 May 2020) and found death rates from swab confirmed COVID-19in that population (12/10387; 0.12%), similar to the local population (4131/7 415 149; 0.12%), compiled from a regional COVID-19 test database. We then audited follow-up patients with a recorded mobile phone number for clinician contact by a linked mobile-phone short message service (SMS) message, previously described.3 This method enables rapid response and provides a feedback rate comparable to …
OP0067 Usefulness of the basal and pilocarpine-stimulated salivary flows in primary sjÖgren’s syndrome
Authors: J Rosas, M Ramos-Casals, J Ena, M Garcia-Carrasco, J Verdu, R Cervera, J Font, O Caballero, M Ingelmo, E Pascual
Journal: Annals of the Rheumatic Diseases (2001)

<h3>Background</h3> <h3>Objectives</h3> To examine salivary function in patients with primary Sjögren's syndrome (SS) by assessing unstimulated and stimulated flows using a 2% pilocarpine solution. <h3>Methods</h3> We investigated the clinical and immunological characteristics of 60 consecutive patients with primary SS. All patients fulfilled four or more of the preliminary diagnostic European criteria for SS. We measured unstimulated (basal) salivary flows (BSF) in all patients. In those patients with a BSF &lt; 1.5 mL, stimulated salivary flows (SSF) were also measured after the stimulus with an ophthalmic 2% pilocarpine solution. <h3>Results</h3> Of the 60 patients, 55 were women and 5 men, with a mean age at the SS onset of 61 years (range 18 to 82 years). The mean BSF for SS patients was 1.40 ± 0.17 mL. Fifty (83%) patients showed a BSF less than 1.5 mL. No clinical or immunological differences were found between SS patients with BSF more or less than 1.5 mL, although those patients with a BSF less than 1.5 mL showed more frequently a parotid scintigraphy class III or IV (42% vs 0%, p = 0.01). Finally, those SS patients with a pilocarpine-SSF less than 1.5 mL showed a higher time of SS evolution (73.3 vs 31.3 months, p = 0.03) and a higher prevalence of positive anti-Ro/SS-A (70% vs 36%, p = 0.038), anti-La/SS-B (65% vs 32%, p = 0.038), parotid scintigraphy class III-IV (79% vs 9%, p &lt; 0.001) and positive salivary gland biopsy (90% vs 43%, p &lt; 0.001). <h3>Conclusion</h3> The study of xerostomia using basal and pilocarpine-stimulated salivary flows are methods simple to perform and acceptable to patients with no special equipment. Twenty-two of the 46 patients with low BSF obtained stimulated flows over 1.5 mL These "responders" patients showed a lower time of evolution of sicca symptomatology, a lower frequency of positive immunological markers and milder grades of scintigraphic patterns and lymphocytic infiltrates on salivary gland biopsies.
Abstract 13405: Interleukin-19 is a Novel Immunoregulatory, Pro-Angiogenic Adipokine
Authors: Christine Vrakas, Allison Herman, Sheri E Kelemen, Rosario G Scalia, Michael Autieri
Journal: Circulation (2019)

Background: The expanding white adipose tissue (WAT) of the obese organism lacks adequate angiogenesis thus becoming hypoxic. Hypoxia results in WAT fibrosis and over production of inflammatory cyt...
Plasma adenosine is linked to disease activity and response to treatment in patients with chronic spontaneous urticaria
Authors: Manyun Mao, Hong Liu, Siyu Yan, Yan Yuan, Runqiu Liu, Yingfang Wu, Cong Peng, Jie Li, Xiang Chen
Journal: Allergy (2020)

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Sponges with Janus Character from Nanocellulose: Preparation and Applications in the Treatment of Hemorrhagic Wounds
Authors: Huan Cheng, Dongdong Xiao, Yujing Tang, Bijia Wang, Xueling Feng, Mujun Lu, G. Julius Vancso, Xiaofeng Sui
Journal: Advanced Healthcare Materials (2020)

Abstract The development of a rapid and effective hemostatic dressing is highly desired in the treatment of hemorrhagic wounds. In this study, sponges with Janus character are developed using cellulose nanofibers (CNFs) that exhibit materials facets of different wettability characteristics using heterogeneous mixing and freeze−drying. The bonding of the interface between the hydrophilic and hydrophobic facets is achieved by using interpenetrating chemical cross‐linking between CNFs and organosilanes. The hydrophilic layer absorbs water from blood and works synergistically with the inherent hemostatic chitosan‐rich complementary layer to accelerate blood clotting, displaying both active and passive hemostatic mechanisms. The hydrophobic layer prevents blood penetration into the construct and exerts proper pressure on the wound. Compared with the hydrophilic control samples and commercial gauzes, the Janus sponges can achieve effective bleeding control with nearly 50% less blood loss in a femoral artery injury model and prolong the survival time in a carotid artery injury model. Compared with the only hydrophilic layer, the time to hemostasis of Janus sponge are reduced from 165 ± 20 to 131 ± 26 s in femoral artery injury model and from 102 ± 21 to 83 ± 15 s in liver femoral artery injury model.
Derivation and validation of a clinical prediction rule for thrombolysis-associated major bleeding in patients with acute pulmonary embolism: the BACS score
Authors: Luis Jara-Palomares, David Jiménez, Behnood Bikdeli, Alfonso Muriel, Parth Rali, Yugo Yamashita, Takeshi Morimoto, Takeshi Kimura, Raphael Le Mao, Antoni Riera-Mestre, Ana Maestre, Fares Moustafa, Manuel Monreal
Journal: European Respiratory Journal (2020)

Improved prediction of the risk of major bleeding in patients with acute pulmonary embolism (
Apoptosis of hematopoietic progenitor-derived adipose tissue–resident macrophages contributes to insulin resistance after myocardial infarction
Authors: Sathish Babu Vasamsetti, Emilie Coppin, Xinyi Zhang, Jonathan Florentin, Sasha Koul, Matthias Götberg, Andrew S. Clugston, Floyd Thoma, John Sembrat, Grant C. Bullock, Dennis Kostka, Claudette M. St. Croix, Ansuman Chattopadhyay, Mauricio Rojas, Suresh R. Mulukutla, Partha Dutta
Journal: Science Translational Medicine (2020)

Patients with insulin resistance have high risk of cardiovascular disease such as myocardial infarction (MI). However, it is not known whether MI can initiate or aggravate insulin resistance. We observed that patients with ST-elevation MI and mice with MI had de novo hyperglycemia and features of insulin resistance, respectively. In mouse models of both myocardial and skeletal muscle injury, we observed that the number of visceral adipose tissue (VAT)-resident macrophages decreased because of apoptosis after these distant organ injuries. Patients displayed a similar decrease in VAT-resident macrophage numbers and developed systemic insulin resistance after ST-elevation MI. Loss of VAT-resident macrophages after MI injury led to systemic insulin resistance in non-diabetic mice. Danger signaling-associated protein high mobility group box 1 was released by the dead myocardium after MI in rodents and triggered macrophage apoptosis via Toll-like receptor 4. The VAT-resident macrophage population in the steady state in mice was transcriptomically distinct from macrophages in the brain, skin, kidney, bone marrow, lungs, and liver and was derived from hematopoietic progenitor cells just after birth. Mechanistically, VAT-resident macrophage apoptosis and de novo insulin resistance in mouse models of MI were linked to diminished concentrations of macrophage colony-stimulating factor and adiponectin. Collectively, these findings demonstrate a previously unappreciated role of adipose tissue-resident macrophages in sensing remote organ injury and promoting MI pathogenesis.
Lentiviral shRNA screen of multidrug resistant associated genes identifies PRP-4 as a new regulator of chemoresistance in human ovarian cancer
Authors: Zhenfeng Duan, Edward Weinstein, Diana Ji, Rachel Ames, Edwin Choy, Henry Mankin, Francis Hornicek
Journal: Cancer Research (2008)

720  Experimental evidence implicates a wide range of mechanisms that may contribute to the drug resistant phenotype in ovarian cancer. The major goal of this study is to determine the mechanism underlying the relationship between overexpression of drug resistance associated genes and multi-drug resistance in ovarian cancer. Using lentiviral shRNAs targeting 120 genes identified from transcriptionally profiling drug resistant cancer cell lines, individual knockdown experiments were performed in the presence of sublethal doses of paclitaxel. Specific knockdown associated cellular toxicity identified ABCB1 (MDR1), survivin and PRP-4 (Pre-mRNA Processing factor-4) as genes directly linked to paclitaxel resistance in the paclitaxel resistant cell line SKOV-3TR. Both ABCB1 and survivin have been previously reported to play a role in multidrug resistance and chemotherapy drug induced apoptosis, while the effects of PRP-4 expression with drug sensitivity is currently unrecognized. PRP-4 belongs to the Ser/Thr protein kinase family, plays a role in pre-mRNA splicing, and interacts with CLK1. Northern analysis demonstrated that PRP-4 is overexpressed in several paclitaxel-resistant cell lines and also confirmed PRP-4 expression could be significantly knocked down by PRP-4 lentivrial shRNA. Both clonegenic and MTT assays demonstrated that knock down of PRP-4 could reverse paclitaxel resistance 5-10 fold in the paclitaxel resistant cell line SKOV-3TR.  Finally, overexpresion of PRP-4 in drug sensitive cells could induce a modest level of drug resistance to paclitaxel.
Trends in the Access and Cost of Photodynamic Therapy Among Medicare Beneficiaries in the United States, 2012-2017
Authors: Shayan Cheraghlou, Hao Feng, Jeffrey M. Cohen
Journal: JAMA Dermatology (2020)

This cross-sectional study evaluates the geographical variation in the availability of photodynamic therapy for the Medicare population in the United States and characterizes the temporal trends of photodynamic therapy cost and use from 2012 to 2017.
Tropism-facilitated delivery of CRISPR/Cas9 system with chimeric antigen receptor-extracellular vesicles against B-cell malignancies
Authors: Qian Xu, Zheng Zhang, Lei Zhao, Yun Qin, Haodong Cai, Zhe Geng, Xiaojian Zhu, Wei Zhang, Yuanyuan Zhang, Jiaqi Tan, Jue Wang, Jianfeng Zhou
Journal: Journal of Controlled Release (2020)
Stress induces NF-κB mediated resistance to Etoposide in H1299 human lung cancer cells
Authors: Irini S. Hadjisavva, Katherine A. Kennedy
Journal: Cancer Research (2004)

1227  Conditions within solid tumor microenvironments can activate stress response pathways leading to the development of resistance to anti-cancer drugs. We show that treatment with the physiologic stress hypoxia or chemical stress Brefeldin A (BFA) leads to the development of resistance to the topoisomerase II inhibitor etoposide in H1299 (p53-/-) human lung cancer cells. Furthermore, experiments measuring luciferase expression after transient transfection of an NF-κB-regulated luciferase reporter construct demonstrate that 4 or 8 hours of hypoxia treatment, or 2 hours BFA followed by 2 hours of BFA-free recovery induce NF-κB activation in H1299 (p53-/-) cells. Experiments using H1299 cells transfected with wild-type p53 in a tetracycline inducible system showed wild-type p53 expressing cells to be similarly resistant to etoposide as their p53-/- counterparts. Finally, we created 5 subpopulations using H1299 cells, which were either non-treated (O2), or treated with 25μM etoposide (O2+25μM E), hypoxia (H), hypoxia and 25μM etoposide (H+25μM E), hypoxia and 100μM etoposide (H+100μM E). H+25μM E represents the equidose subpopulation and H+100μM E represents the equitoxic dose subpopulation to the O2+25μM E treated population. Preliminary data using these subpopulations demonstrate that in non-stressful conditions (95% air/5% CO2), the O2+25μM E subpopulation is greater than 3-fold resistant to etoposide than the O2 controls. In contrast, the sensitivity to etoposide is similar to that of O2 control populations in the H, H+25μM E and H+100μM E subpopulations. Furthermore, experiments show the hypoxic-treated subpopulations to be intrinsically less sensitive to etoposide in the presence of stress than the O2 and O2+25μM E subpopulations. Taken together our findings demonstrate an involvement of NF-κB in the development of stress-induced drug resistance in human H1299 (p53-/-) cells, and suggest that the resistance pathway is independent of p53. Moreover, data from the H1299 subpopulations suggest that the hypoxia-induced resistance pathway suppresses the resistance to drug caused by prior drug exposure, and further that hypoxia pretreatment makes H1299 subpopulations intrinsically less sensitive to etoposide. Understanding the pathways by which stress-induced resistance to anti-cancer drugs operate could be important for the development of therapeutic approaches for solid tumors. Supported by Army Breast Cancer Initiative Award 99-1-9186 (K.A.K.)
Effect of Different Treatment Patterns to Cost Effectiveness in Chronic Lymphocytic Leukemia Patients, Turkish CLL Study Group
Authors: Fatih Demirkan, Filiz Vural, Atakan Tekinalp, Omur Gokmen Sevindik, Nilgun Sayinalp, Leylagul Kaynar, Mehmet Ali Erkurt, Sibel Kabukcu Hacioglu, Munci Yagci, Burhan Turgut
Journal: Blood (2017)
Single-Cell Reconstruction of Human Basal Cell Diversity in Normal and Idiopathic Pulmonary Fibrosis Lungs
Authors: Gianni Carraro, Apoorva Mulay, Changfu Yao, Takako Mizuno, Bindu Konda, Martin Petrov, Daniel Lafkas, Joe R. Arron, Cory M. Hogaboam, Peter Chen, Dianhua Jiang, Paul W. Noble, Scott H. Randell, Jonathan L. McQualter, Barry R. Stripp
Journal: American Journal of Respiratory and Critical Care Medicine (2020)

Rationale: Declining lung function in patients with interstitial lung disease is accompanied by epithelial remodeling and progressive scarring of the gas-exchange region. There is a need to better understand the contribution of basal cell hyperplasia and associated mucosecretory dysfunction to the development of idiopathic pulmonary fibrosis (IPF).Objectives: We sought to decipher the transcriptome of freshly isolated epithelial cells from normal and IPF lungs to discern disease-dependent changes within basal stem cells.Methods: Single-cell RNA sequencing was used to map epithelial cell types of the normal and IPF human airways. Organoid and air-liquid interface cultures were used to investigate functional properties of basal cell subtypes.Measurements and Main Results: We found that basal cells included multipotent and secretory primed subsets in control adult lung tissue. Secretory primed basal cells include an overlapping molecular signature with basal cells obtained from the distal lung tissue of IPF lungs. We confirmed that NOTCH2 maintains undifferentiated basal cells and restricts basal-to-ciliated differentiation, and we present evidence that NOTCH3 functions to restrain secretory differentiation.Conclusions: Basal cells are dynamically regulated in disease and are specifically biased toward the expansion of the secretory primed basal cell subset in IPF. Modulation of basal cell plasticity may represent a relevant target for therapeutic intervention in IPF.
American College of Rheumatology Guidance for the Management of Pediatric Rheumatic Disease During the COVID‐19 Pandemic: Version 1
Authors: Dawn M. Wahezi, Mindy S. Lo, Tamar B. Rubinstein, Sarah Ringold, Stacy P. Ardoin, Kevin J. Downes, Karla B. Jones, Ronald M. Laxer, Rebecca Pellet Madan, Amy S. Mudano, Amy S. Turner, David R. Karp, Jay J. Mehta
Journal: Arthritis & Rheumatology (2020)

To provide clinical guidance to rheumatology providers who treat children with pediatric rheumatic disease (PRD) in the context of the coronavirus disease 2019 (COVID-19) pandemic.The task force, consisting of 7 pediatric rheumatologists, 2 pediatric infectious disease physicians, 1 adult rheumatologist, and 1 pediatric nurse practitioner, was convened on May 21, 2020. Clinical questions and subsequent guidance statements were drafted based on a review of the queries posed by the patients as well as the families and healthcare providers of children with PRD. An evidence report was generated and disseminated to task force members to assist with 3 rounds of asynchronous, anonymous voting by email using a modified Delphi approach. Voting was completed using a 9-point numeric scoring system with predefined levels of agreement (categorized as disagreement, uncertainty, or agreement, with median scores of 1-3, 4-6, and 7-9, respectively) and consensus (categorized as low, moderate, or high). To be approved as a guidance statement, median vote ratings were required to fall into the highest tertile for agreement, with either moderate or high levels of consensus.The task force drafted 33 guidance statements, which were voted upon during the second and third rounds of voting. Of these 33 statements, all received median vote ratings within the highest tertile of agreement and were associated with either moderate consensus (n = 6) or high consensus (n = 27). Statements with similar recommendations were combined, resulting in 27 final guidance statements.These guidance statements have been generated based on review of the available literature, indicating that children with PRD do not appear to be at increased risk for susceptibility to SARS-CoV-2 infection. This guidance is presented as a "living document," recognizing that the literature on COVID-19 is rapidly evolving, with future updates anticipated.
Molecular basis of cancer cell resistance to allyl isothiocyanate-induced growth inhibition
Authors: Joseph D. Paonessa, Li Tang, Yuesheng Zhang
Journal: Cancer Research (2005)

5859  In addition to exerting their anticarcinogenic activity by modulating carcinogen-metabolizing enzymes, many isothiocyanates (ITCs) also inhibit the growth of various types of cancer cells through induction of apoptosis, arrest of cell cycle progression and induction of differentiation, demonstrating their cancer therapeutic potential. However, there is significant variation in the sensitivity of cancer cell lines to the growth-inhibitory effects of ITCs, and the reason is poorly understood. Human hepatoma HepG2 cells were 6-8 times more resistant to allyl ITC (AITC) than human prostate cancer LNCaP cells, based on their IC50 values. The uptake levels of AITC in both cell lines were similar. AITC-induced cell cycle arrest does not fully account for the marked difference in growth inhibition of the two cell lines either. For example, 37% of LNCaP cells and 20% of HepG2 cells were blocked in G2/M phase after treatment with 18 μM AITC for 24 h. In contrast, AITC-induced cell death, as measured by the occurrence of sub-G1 phase cells, was markedly higher in LNCaP cells than in HepG2 cells - 47% of LNCaP cells and only 5% of HepG2 cells were in Sub-G1 phase, after treatment with 18 μM AITC for 24 h. The differential effects of AITC on cell death were closely correlated with the susceptibility of the mitochondrial membrane to AITC. For example, whereas treatment of LNCaP cells with 18 μM AITC for 1 h and 3 h caused a decrease in mitochondrial trans-membrane potential in 23% and 36% of the cells, respectively, the same treatment had no effect on the mitochondrial trans-membrane potential in HepG2 cells. Bcl-2 family proteins are known to affect the stability of the mitochondrial membrane. Indeed, the expression levels of Bcl-2 and Bcl-xl, both of which are known to stabilize the mitochondrial membrane, are markedly higher in HepG2 cells, whereas Bad, which is known to destabilize the mitochondrial membrane, is significantly higher in LNCaP Cells. These results suggest that Bcl-2 family proteins affect AITC-induced mitochondrial membrane damage. The cytoprotective function of glutathione (GSH) is well known. Total intracellular GSH level in LNCaP cells (28 nmol/mg crude cell lysate protein) is approximately half of that in HepG2 cells (54 nmol/mg protein). However, while it is not yet clear to what extent the difference of GSH content in these cells contribute to the difference in AITC-induced cell death, we found that GSH depletion in HepG2 cells, through inhibition of GSH biosynthesis, did not sensitize the mitochondria in these cells to AITC. Taken together, these results suggest that the mitochondria play a major role in determining the sensitivity of cancer cells to AITC-induced cytotoxicity. Supported in part by NCI grant CA80962.
Early Cardiovascular Toxicity with Treatment of Follicular Lymphoma Is Associated with Shorter Overall Survival
Authors: Suchitra Sundaram, Gouri Dharmavaram, Benjamin K. Tomlinson, Richard Creger, Ehsan Malek, Leland Metheny, Brenda W. Cooper, Hillard M Lazarus, Marcos de Lima, Paolo Caimi
Journal: Blood (2017)
Hypoxia induced exosomal circRNA promotes metastasis of Colorectal Cancer via targeting GEF-H1/RhoA axis
Authors: Haiou Yang, Haiyang Zhang, Yuchong Yang, Xinyi Wang, Ting Deng, Rui Liu, Tao Ning, Ming Bai, Hongli Li, Kegan Zhu, Jialu Li, Qian Fan, Guoguang Ying, Yi Ba
Journal: Theranostics (2020)

Hypoxia is one of the important properties of solid tumor. However, oxygen supply within tumors is generally heterogeneous according to the distance from the nearest blood vessel. The discrepancy of metastatic potential exists between hypoxic cancer cells and relatively normoxic cancer cells. But the molecular mechanism remains poorly understood.
Surgical Jacket and Bouffant Use and Surgical Site Infection Risk
Authors: Lisa Spruce
Journal: JAMA Surgery (2020)

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