AB0037 Mir-155 promotes the differentiation of th17 cell by targeting ets-1 in rheumatoid arthritis
Authors: Z. Yin, W. He, X. Chen, X. Luo, Y. Zhang, Z. Ye
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> MicroRNAs play important roles in arthritis. MiR-155 emerges as a central regulator of the immune response, but its function in autoimmune arthritis is poorly understood. <h3>Objectives</h3> To clarify the role of miR-155 in the balance of CD4<sup>+</sup>Th17 and Treg cell differentiations as well as how their mechanisms function in autoimmune arthritis. <h3>Methods</h3> CD4 +T cells were <i>in vitro</i> isolated from DBA1 mice. The transfections of miR-155 mimics and miR-155 inhibitors in CD4 +T cells were performed for enhanced or knockdowns of miR-155 expression and RNA interference was applied to silence Ets-1 gene expression. In addition, we carried out <i>in vivo</i> experiments of enhanced miR-155 expression by lenti-virus mediated miR-155 (LV-miR-155) infection and silencing of miR-155 expression by lenti-virus-anti-miR-155(LV-anti-miR-155) within our well-established type II collagen (CII)-induced arthritis (CIA) mice model. The ratios of IL-17<sup>+</sup> TH17 cells were analysed using flow cyctometry (FCM). The expressions of some key autoimmune-response of genes including RORgt, IFN-g, Ets-1, IL-17, IL-23, and Stat3 were further determined by (Taqman) Quantitative Real-time PCR and Western blotting. Initially n=6 per group if not indicated further;totally 6 groups in parallel per experiment. *p&lt;0.05, considered as significantly;**p&lt;0.01, considered as highly significantly. <h3>Results</h3> For the first time we showed that miR-155 may promote Th17 cell differentiation in RA pathogenesis. Firstly, we observed that miR-155 may significantly induce the expressions of some key autoimmune-response genes including RORgt, IFN-g, IL-17, IL-23, and Stat3 but it significantly inhibited Ets-1 gene expression. 2ndly, miR-155 may in part modulates Th17 cell differentiation by targeting Ets-1 in that indeed the expression of miR-155 significantly co-related with disease index in CIA mice. Furthermore, the CIA mice with <i>in vivo</i> forced expression of miR-155 have significantly more Th17 cells(i.e.high ratio) and severe CIA disease index compared to their control CIA mice;strikingly, they also have a significantly higher expression of aforementioned autoimmune-response genes compared to their corresponding controls along with the response of Ets-1 exactly in a verse direction. Consistently, the CIA mice with <i>in vivo</i> knockdown of miR-155 expression resulted in significantly less Th17 cells and lesser severe CIA disease index compared to their control CIA mice. However, they have a significantly lower expression of above-mentioned autoimmune-response genes compared to their corresponding controls along with a response of Ets-1 in a verse direction (the data in part shown in Figure 1;sham=CIA treated with LV-nonspecific sequence). <h3>Conclusions</h3> For the first time our data show miR-155 has a critical role in Th17 differentiation and the RA pathogenesis via targeting the Ets-1. Although it warrants further investigations, miR-155 might be a promising therapeutic target for autoimmune diseases, esp. RA. <h3>Acknowledgements</h3> This work ws supported by National Natural Science Foundation of China #81102266. All authors listed were either involved in conception and design, or acquisition, analysis and interpretation of data, and/or writing. Each author gave their final approval of this version. <h3>Disclosure of Interest</h3> None declared