tbuckley/cabot-search huggingface dataset:
Antitumor effect of preparation for colon cancer cells with hyperthermia and/or Hsp70 inducer, geranylgeranylacetone in pulmonary metastasis models of colon cancer and subcutaneous tumor model in mice
Authors: Tetsuya Okayama, Satoshi Kokura, Toshimitsu Okuda, Satoko Adachi, Takeshi Hattori, Nami Nakabe, Naoyuki Sakamoto, Osamu Handa, Tomohisa Takagi, Yuji Naito, Norimasa Yoshida, Haruki Kato, Toshikazu Yoshikawa
Journal: Cancer Research (2007)

2203  Aim: The transcription factor-kB(NF-kB)is activated in many human cancers, and induces the expression of multiple genes including CyclinD1, COX-2, VEGF, Bcl-2, and IAP. Therefore, the blocking of NF-kB activity may be associated with the suppression of proliferation, angiogenesis, and induction of apoptosis.  Recently, we confirmed heat treatment inhibited activation of NF-kB through the expression of heat shock proteins in a human colon cancer cell line.  In clinically, it is known that chemotherapy for cancer growing at several local areas induces remote metastasis. The part of the reason is thought that NF-kB is activated by chemotherapy. We reported previously that hyperthermia and induction of Hsp70 inhibited continuous activation of NF-kB of cancer cells. In this study, we examined whether hyperthermia attenuated the metastatic potential of colon cancer through the induction of Hsp70.  Method: Colon26 cell, a mouse colon adenocarcinoma cell line, were separated four groups. 1) no treatment, 2) treatment with hyperthermia at 42 degree Celsius in the CO2 incubator for one hour, 3) treatment with Hsp inducer, geranylgeranylacetone (GGA, 10-6M) for two hours, 4) treatment with hyperthermia after GGA treatment. We measured the viability of each-treated colon26 cells by WST-8 and the content of Hsp 70 in each-treated cell by Western blotting.  For in vivo study, colon26 cells in four groups were injected via the tail vein or into the subcutaneous zone of male BALB/c mice and numbers of pulmonary metastases or size of subcutaneous tumors were assessed 14 days later.  Results: Cell viability is almost equal in four groups. About the induction of Hsp70 in colon26 cells, GGA and hyperthermia induced Hsp70 as compared with untreated colon26. The combination of GGA and hyperthermia further induced Hsp70. In pulmonary metastasis models, the combination of GGA and hyperthermia is less pulmonary metastases than other cell groups. In subcutaneous tumor models, all cell groups were developing as same.  Conculusion: It is suggested that the metastatic potential but not the proliferation potency of cancer cells is inhibited by transient induced Hsp70.
AB1473-HPR Quality of referral letters received in rheumatology
Authors: D Palma-Sanchez, A.D.C. Haro-Martinez, M.J. Moreno-Martinez, E. Peñas-Gonzalez
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> The referral letter is an essential tool in the relationship between Primary Care and other specialties with Rheumatology. In our area, in 2010, a computer system called SELENE was introduced at hospital level and, in Primary Care, O.M.I. was set up more than 20 years ago <h3>Objectives</h3> To assess the quality of referral letters received in the Rheumatology Unit during the first four months of 2017 <h3>Methods</h3> Retrospective and descriptive study, in which referral letters received in the Rheumatology Unit between January and April 2017 were analysed. The following variables were collected: medical specialty that sent the document, computerization of the document, reference to medical history, chronic treatments, current disease, physical examination, complementary tests, presumption diagnosis, treatment for the current process, and number of reasons for consultation per document. The quality of the document was assessed using the recommendations of Irazábal and Gutiérrez modified by Morera et al, which classified them as: good, acceptable and bad (table 1). <h3>Good</h3> It includes medical history, familiar medical history, chronic treatment, current disease, complementary tests, presumption diagnosis, differential diagnosis and reason for consultation <h3>Acceptable</h3> It includes reason for consultation and enough information about current disease, although not all those included in the previous one <h3>Bad</h3> Unreadable, without enough information to assess the current disease or absence of information In the descriptive statistical analysis of quantitative variables, the mean and standard deviation were used. Frequencies were used to analyse qualitative variables <h3>Results</h3> 1234 referral letters were included and 90.3% were computerised. The specialties that referred patients more frequently were: Primary Care (84.7%), Traumatology (3.4%), Neurology (1.9%) and Emergency (1.6%). 14.5% of the documents included more than one reason for consultation. The variables related to the fulfilment of quality criteria in the referral letters are shown in Table 2. Regarding the quality level, 4.1% of the documents were good, 95.2% acceptable and 0.6% bad. <h3>Conclusions</h3> The quality of the referral letters was mostly acceptable. Therefore, there is much room to improve, especially in the sections referencing medical history, chronic treatment, physical examination and treatment for the current process <h3>Disclosure of Interest</h3> None declared
AB1180 The influence of mechanical stress on the hands on ultrasound results: a prospective study with volleyball players (US-VOLLEY) – preliminary data
Authors: D. Scheicht, S. Zeglam, S. Frechen, J. Strunk
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Various imaging studies have found an influence of physical exercise on joint and tendon pathology in ultrasound. It is not clear<sup>1</sup> how specific grey scale and power Doppler changes are in a context of mechanical stress and<sup>2</sup> if a distinction to findings in acute arthritis exists. <h3>Objectives</h3> To examine via grey scale and power Doppler ultrasound the acute physiological effects of increased physical stress on the fingers and wrists and their surrounding structures through volleyball training in amateur healthy subjects as a reference to the known pathological findings (effusion, increased perfusion) in acute arthritis. <h3>Methods</h3> Examination of 18 healthy amateur volleyball players via musculoskeletal ultrasound before and directly after their routine volleyball practice. Ultrasound assessment included a grey scale and power Doppler examination of the dominant hand (longitudinal planes of the dorsal wrist and dorsal and palmar planes of MCP II-III and PIP II-III joints). Findings were graded semi-quantitatively from 0 to 3 both in grey scale (effusion) and power Doppler (perfusion). The results of the joint assessment were aggregated in a composite index for grey scale and power Doppler (range 0–54 points). All ultrasound images were graded by two independent raters who were blinded to image acquisition. <h3>Results</h3> Volleyball training showed statistically significant effects in the composite index for grey scale and power Doppler scores (p=0.004). 16 of 18 players showed a change in their composite index with a median change of 1 and a maximum change of 3 points. Subanalysis revealed that changes were related to grey scale exclusively, with no statistical difference in power Doppler scores. <h3>Conclusions</h3> The results of our study suggest that mechanical stress on the hands leads to changes in grey scale ultrasound in healthy subjects in at least one joint. However, the composite index for changes in grey scale and power Doppler changed 3 points at the most in one subject over various joints. To add, no changes in power Doppler score were observed. While changes in grey scale ultrasound appear to be minor, power Doppler ultrasound appears to be a more robust method and less prone to environmental factors. Power Doppler appears to be able to discriminate between physiological changes due to mechanical stress and acute arthritis and thus, is highly specific. <h3>Disclosure of Interest</h3> None declared
THU0360 A validation study of the global antiphospholipid syndrome score (GAPSS) in women with systemic lupus erythematosus and pregnancy morbidity
Authors: K. Schreiber, M. Radin, I. Cecchi, M.J. Cuadrado, L. Marozio, D. Roccatello, S. Sciascia
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Systemic lupus erythematosus (SLE) and antiphospholipid antibodies (aPL) are associated with pregnancy complications. <h3>Objectives</h3> To validate the global antiphospholipid syndrome score (GAPSS) in a cohort of women with SLE. <h3>Methods</h3> 143 women ever pregnant with SLE who presented in our outpatient clinic were included (table 1). Data on cardiovascular risk factors and aPL positivity were retrospectively collected. The individual GAPSS was calculated for each patient by calculating the sum of each risk factor score, which is based on a linear transformation derived from the ß regression coefficient as follows: 3 for hyperlipidaemia, 1 for arterial hypertension, 5 for aCL IgG/IgM, 4 for anti-b2 glycoprotein I IgG/IgM, 3 for aPS/PT IgG/M and 4 for lupus anticoagulant (LA)<sup>1</sup>. <h3>Results</h3> Significantly higher GAPSS values were seen in patients with a history of pregnancy complications compared to those wihout a history pregnancy complications. Results are outlined in table 1 and figure 1. <h3>Conclusions</h3> Higher GAPSS values are found in women with SLE and aPL with previous pregnancy complications compared to those without pregnancy complications. The clinical utility of the GAPSS score in pregnancy seem promising and should be validated in a prospective cohort. <h3>Reference</h3> [1] Sciascia S, et al. GAPSS: the Global Anti-Phospholipid Syndrome Score. Rheumatology (Oxford)2013;52:1397–1403. doi:10.1093/rheumatology/kes388 <h3>Disclosure of Interest</h3> None declared
Acetyl-CoA Metabolism Supports Multistep Pancreatic Tumorigenesis
Authors: Alessandro Carrer, Sophie Trefely, Steven Zhao, Sydney L. Campbell, Robert J. Norgard, Kollin C. Schultz, Simone Sidoli, Joshua L.D. Parris, Hayley C. Affronti, Sharanya Sivanand, Shaun Egolf, Yogev Sela, Marco Trizzino, Alessandro Gardini, Benjamin A. Garcia, Nathaniel W. Snyder, Ben Z. Stanger, Kathryn E. Wellen
Journal: Cancer Discovery (2019)

Pancreatic ductal adenocarcinoma (PDA) has a poor prognosis, and new strategies for prevention and treatment are urgently needed. We previously reported that histone H4 acetylation is elevated in pancreatic acinar cells harboring
Altered DNA methylation in children born to mothers with rheumatoid arthritis during pregnancy
Authors: Hilal Ince-Askan, Pooja R Mandaviya, Janine F Felix, Liesbeth Duijts, Joyce B van Meurs, Johanna M W Hazes, Radboud J E M Dolhain
Journal: Annals of the Rheumatic Diseases (2019)

The main objective of this study was to determine whether the DNA methylation profile of children born to mothers with rheumatoid arthritis (RA) is different from that of children born to mothers from the general population. In addition, we aimed to determine whether any differences in methylation are associated with maternal RA disease activity or medication use during pregnancy.For this study, genome-wide DNA methylation was measured at cytosine-phosphate-guanine (CpG) sites, using the Infinium Illumina HumanMethylation 450K BeadChip, in 80 blood samples from children (mean age=6.8 years) born to mothers with RA. As controls, blood samples from 354 children (mean age=6.0 years) from the population-based Generation R Study were used. Linear mixed models were performed to investigate differential methylation between the groups, corrected for relevant confounders.A total of 147 CpGs were differentially methylated between blood samples of children born to mothers with RA and the control blood samples. The five most significantly associated CpGs were cg06642177, cg08867893, cg06778273, cg07786668 and cg20116574. The differences in methylation were not associated with maternal RA disease activity or medication use during pregnancy.DNA methylation at 147 CpGs differed between children born to mothers with RA and children born to mothers from the general population. It remains unknown whether the identified associations are causal, and if so whether they are caused by the disease or treatment. More research, including replication of these results, is necessary in order to strengthen the relevance of our findings for the later-life health of children born to mothers with RA.
BRD4 Degradation Is a Potent Approach to Block MYC Expression and to Overcome Multiple Forms of Stem Cell Resistance in Ph+ CML
Authors: Barbara Peter, Gregor Eisenwort, Alexandra Keller, Karin Bauer, Daniela Berger, Irina Sadovnik, Gabriele Stefanzl, Gregor Hoermann, Dominik Wolf, Zdenek Racil, Jiří Mayer, Johannes Zuber, Wolfgang R. Sperr, Georg E. Winter, Michael Willmann, Thomas Rülicke, Peter Valent
Journal: Blood (2018)
THU0174 Sexual function of women with rheumatoid arthritis in comparison with healthy volunteers
Authors: A. Fazaa, R. Ben Saad, K. ouennich, S. Miladi, S. kassab, C. salma, K. Ben Abdelghani, A. laatar
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Rheumatoid Arthritis (RA) is one of the first diseases where quality of life (QoL) measurements are considered as a therapeutic goal. Sexual health is an important part of a quality of life. Persons with RA often experience decreased sexual health, due to pain, fatigue and physical disability. <h3>Objectives</h3> To assess the prevalence of sexual dysfunction in married women with RA and to compare it with a control group. <h3>Methods</h3> We conducted a cross-sectional study including seventy one married women with RA (ACR/EULAR Criteria), having sexual activity and seventy one, healthy volunteers women matched for socio-demographic characteristics. Sexual function was assessed by a self-reported questionnaire the Index of Female Sexual Function (FSFI). The comparison of qualitative variables was performed with the Chi square test and the comparison of qualitative variable and quantitative ones was performed with the Student's test. The significance level was set at 0.05. <h3>Results</h3> The prevalence of female sexual dysfunction in women with RA and in controls was 49% and 23,9% respectively. There was a significant difference in the total FSFI score between patients and controls (24±6,7 versus 27,05±5,34; p=0,002). We found statistically significant differences between the two groups in dimensions of sexual function: desire (p=0,050), arousal (p=0,038) and satisfaction (p=0,024). However, no significant differences were found for pain (p=0,757), lubrication (p=0,069) and orgasm (p=0,083). <h3>Conclusions</h3> Our findings showed that RA adversely affects women's sexual function. The FSFI, easy and quick to use, could be proposed for the assessment of female sexual function to optimise the management of patients with RA. <h3>Disclosure of Interest</h3> None declared
AB0749 Don’t forget the caregivers of patients with systemic sclerosis
Authors: M. Boonstra, D.F. Stoop, T.W. Huizinga, T.P. Vliet Vlieland, J.K. de Vries-Bouwstra
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Systemic sclerosis (SSc) is a disease with considerable physical and psychosocial impact. Previous studies among partners of patients with other chronic diseases, such as Parkinson's and stroke, showed that 6%–25% of caregivers experience high levels of stress related to caregiving. To our knowledge, the burden experienced by caregivers of patients with SSc has not been determined so far. <h3>Objectives</h3> To evaluate perceived strain, quality of life and health care needs among SSc caregivers. <h3>Methods</h3> During a patient information meeting, companions of SSc patients were invited to complete 3 questionnaires: 1. The Caregiver Strain Index questionnaire (CSI), in which a score ≥7 indicates high care burden, 2. Short Form-36 (SF-36): a measure of self-reported Quality of Life (QoL) and 3. a self-designed questionnaire on the needs and preferences for caregiving support. Caregivers with and without high care burden were compared regarding their general characteristics and Qol. <h3>Results</h3> Thirty-six SSc caregiver filled out questionnaires (n=31 completed the CSI questionnaire). Respondents were mostly men (64%), on average 62 years old (SD 14), 35% (n=11) had a paid job, and 50% (n=18) suffered from chronic disease. The majority was in a spousal relationship (75%) with the SSc patient and 56% of patients had been diagnosed with SSc more than 5 years ago. Nineteen percent of patients (n=7) needed daily personal caregiving and 25% (n=9) was transport-dependent. Median CSI was 2 (range 0 to 5), and 6/31 (19%) perceived high care burden(CSI ≥7). Caregivers with high care burden were significantly more often involved in daily personal caregiving of the patients (3/6 vs. 2/25). Although not statistically significant, all caregivers perceiving a high burden were spouses, while the remaining caregivers had varying relationships with SSc patients. SF-36 summary scale scores were similar in both groups (table 1). Regarding needs and preferences of caregivers, 15% (n=5) reported a need for additional individual information, and 21% (n=7) a need for contact with fellow caregivers of patients, preferably during information meetings. <sup>1</sup><b> Fisher's exact <sup>2</sup> Mann-Withney U *n=35, **n=31, ***n=28, ****n=19</b> <h3>Conclusions</h3> Within a small group of SSc caregivers, 19% experienced high care burden. In particular caregivers involved in personal caregiving seem to be at risk for increased strain related to caregiving. The results of the current study can direct clinical practice aiming at support of the caregivers. <h3>Disclosure of Interest</h3> None declared
Appropriateness of Carotid Endarterectomy in Asymptomatic Carotid Disease—Predicting 5-Year Survival
Authors: Kara A. Rothenberg, Shipra Arya
Journal: JAMA Surgery (2019)

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AB0223 Has the presentation and severity of rheumatoid arthritis changed in recent decades?
Authors: E. Chamizo Carmona, C. Carrasco Cubero, S.M. Rojas Herrera, J. Malave Calzada, L.M. Chaves Chaparro
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> In recent decades, there have been social, economic and health changes, also in the treatment of rheumatoid arthritis (RA). The development and severity of RA can be affected by these changes. It is discussed if RA is currently less frequent and severe than before<sup>1</sup>. <h3>Objectives</h3> To determine the changes in the clinical and demographic variables and the severity of RA that may have occurred over a period of four decades (from 1980 to 2015) and its repercussion in the response to methotrexate (MTX) monotherapy. <h3>Methods</h3> We conducted a retrospective study, reviewing the records of patients from a cohort of RA who received MTX monotherapy and compared the results of demographic and clinical variables by natural decades. The statistical analysis was performed using the Chi2 test and the one-way ANOVA test. <h3>Results</h3> We included 301 patients (202 women and 99 men) with a mean age at diagnosis of 49.6 (±13.2) years. The table 1 shows the characteristics of the complete sample and distributed by natural decades. The age at diagnosis was higher after 2000 (p&lt;0.01), while the proportion of women, educational level, sedentary lifestyle, habits (tobacco, alcohol) and comorbidities did not change. There was also no variation in the joint pattern of presentation, percentage of RF/ACPA positive or mean value of RF and ACPA. There was a decrease in time from the onset of symptoms to diagnosis (from 21.7 to 15.3 months) (NS), but especially between the diagnosis and the first DMARD (from 34.5 to 1.4 months) and in the number of FAMEs before MTX (p&lt;0.01 for both), as well as in the baseline activity of the disease (PCR and DAS28-PCR), early erosions (&lt;2 years) and extra-articular involvement (p&lt;0.01). There were no changes in the dose of prednisone and MTX, nor in the route of administration, while folic acid supplements increased. The suspension rate due to toxicity decreased (p&lt;0.05), while the clinical remission rate improved (p&lt;0.01). <h3>Conclusions</h3> In our cohort, the severity of RA has decreased during the last four decades, the time to start MTX has been reduced and better remission rates with MTX monotherapy have been achieved. <h3>Reference</h3> [1] Silman AJ. Trends in the incidence and severity of rheumatoid arthritis. J Rheumatol1992;19(suppl 32):71–3. <h3>Disclosure of Interest</h3> None declared
SP0061 How to use mri in the diagnosis and management of psoriatic arthritis + clinical cases
Authors: M. Østergaard
Journal: Annals of the Rheumatic Diseases (2018)

<h3></h3> Psoriatic arthritis (PsA) is an inflammatory joint disease characterised by presence of arthritis, enthesitis and/or spondylitis in patients with psoriasis. PsA presents a wide range of disease manifestations in various patterns. Imaging is an important part of management of PsA and is used for multiple reasons including establishing/confirming a diagnosis of inflammatory joint disease, determining the extent of disease, monitoring activity and damage, assessing therapeutic efficacy, and identifying complications of disease or treatment, in the setting of clinical practice or clinical studies. Magnetic resonance imaging (MRI) allows detailed assessment of all peripheral and axial joints and entheses involved in PsA and can visualise both inflammation and structural changes. This talk will provide an overview of the status and perspectives of MRI in diagnosis and management peripheral and axial PsA. <h3>Disclosure of Interest</h3> None declared