tbuckley/cabot-search huggingface dataset:
Evaluation of the Response of Unresectable Primary Cutaneous Melanoma to Immunotherapy Visualized With Reflectance Confocal Microscopy
Authors: Cristian Navarrete-Dechent, Miguel Cordova, Michael A. Postow, Melissa Pulitzer, Cecilia Lezcano, Allan C. Halpern, Anthony M. Rossi
Journal: JAMA Dermatology (2019)

Melanoma incidence and the use of systemic treatments for it are rising. Current treatment monitoring uses clinical examination and radiologic examinations; however, cutaneous involvement and cutaneous metastasis may not be well visualized. Reflectance confocal microscopy (RCM) is a US Food and Drug Administration-approved, noninvasive technology that enables visualization of the skin with quasihistological resolution.To evaluate the feasibility of using RCM to monitor advanced melanomas treated with immunotherapy.This case report study took place from March 2017 to June 2018 and included 2 patients with locally advanced melanoma who were not candidates for surgery or were not willing to have surgery and who were started on an immunotherapy regimen at a tertiary care cancer hospital.Clinical and RCM findings correlated with histopathology.In the patients, locally advanced melanoma with cutaneous involvement was treated with immunotherapy (pembrolizumab in 1 patient and an ipilimumab-nivolumab combination in the other) with resulting clearance of the lesions. Use of RCM showed the disappearance of clear melanoma features seen at baseline; these findings correlated with histopathology. The response was not seen with radiologic images, such as magnetic resonance imaging and computed tomography.Although RCM will not replace larger field imaging (such as magnetic resonance imaging, positron emission tomography, and computed tomography) in the management and follow-up of melanoma or other tumors, for imaging of cutaneous involvement and disease monitoring, RCM holds promise as a novel noninvasive technique.
A sensitive method for quantitative measurement of plasma dna: a marker for cancer progression and treatment
Authors: Mei Zhang, Hengshan Zhang, Aiguo Zhang, Lulu Zhang, Yunqing Ma, Shanmin Yang, Wei Wang, Weimin Sun, Chaomei Liu, Kunzhong Zhang, Liangjie Yin, Bruce Fenton, Peter Keng, Paul Okunieff, Lurong Zhang
Journal: Cancer Research (2008)

3642    Introduction:  Necrosis is a common characteristic of cancer progression, and causes the release of DNA into the circulation. This cell death and release of DNA is due in part to uncontrolled tumor growth overwhelming the capacity of its supply of oxygen and nutrients. The DNA fragments released from spontaneous apoptosis of cancer cells can also enter the circulation and be detected in plasma. While plasma DNA level is likely to be a marker for  cancer progression and treatment, the method for rapid and quantitative measurement of plasma DNA without extraction is not well developed. Recently, we have utilized QuantiGene 2.0 technology to develop a method that can sensitively measure the level of plasma DNA with potential for monitoring cancer progression and treatment.  Methods:  A novel assay based on QuantiGene 2.0 technology was developed. Several capture probes were designed to immobilize the plasma DNA on plate. The bound DNA was detected using several layers of amplifications, i.e. the label extender, preamplifier, amplifier and label probe conjugated with alkaline phosphatase. The substrate Lumigen APS-5 yields chemiluminescence (RLU), which is quantitatively related to the amount of bound DNA. The plasma DNA was measured in plasma sample from  1)  normal adults;  2)  patients with different diseases, including lymphoma, leukemia, liver cancer;  3)  patients with autoimmune disease, such as systemic lupus erythematosus (SLE) which has antibody generated against autologous DNA;  4)  pregnant subjects; and  5)  leukemia patient that had received radiation for bone marrow transplantation (BMT). The significance of results was assessed. Results:  The correlation co-efficient (R value) between RLU and plasma DNA concentration of our assay was 0.9996. While the normal adults had a very low level of plasma DNA, all the cancer patients (lymphoma, leukemia and liver cancer) had much higher (11-26 fold). The patients with SLE also had a high level of plasma DNA as did pregnant women. The leukemia patients who received TBI (1.65 Gy BID x 4 days) prior to BMT had a huge surge (about 140 times normal) of plasma DNA seen immediately after the first dose of total body radiation (TBI), and which remained high during the whole course of radiation. When TBI was stopped on day 6, the level gradually declined to the basal level on day 9. Conclusion: We have developed a highly sensitive method to determine the plasma DNA level. The method can be used for several clinical applications, and has potential for monitoring cancer progression and therapeutic effect. The value of this index will be tested in a large-scale clinical trial.
Consensus Grading of Cytokine Release Syndrome (CRS) in Adult Patients with Relapsed or Refractory Diffuse Large B-Cell Lymphoma (r/r DLBCL) Treated with Tisagenlecleucel on the JULIET Study
Authors: Stephen J. Schuster, Richard T. Maziarz, Solveig G. Ericson, Elisha S. Rusch, James Signorovitch, Vadim V. Romanov, Frederick L. Locke, David G. Maloney
Journal: Blood (2018)
Abstract 17332: A Multi-Marker Approach for Risk Stratification in Patients With Chronic Heart Failure: Insights From the HF-ACTION Trial
Authors: Abhinav Sharma, Susanna R Stevens, Adrian Coles, Mona Fiuzat, Kirkwood F Adams, David J Whellan, Mark P Donahue, Dalane W Kitzman, Ileana L Piña, Faiez Zannad, William E Kraus, Christopher M O'Connor, G. Michael Felker
Journal: Circulation (2017)

Introduction: It is unclear whether multi-marker approaches improve risk stratification among patients with heart failure (HF). We evaluated the prognostic utility of a multi-marker approach with b...
THU0613 The frequency and characteristics of headache in behcet’s disease and its evaluation by transcranial doppler ultrasonography
Authors: F. Salgür, D. Üsküdar Cansu, N. Uzuner, C. Korkmaz
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Behcet's disease (BD) is a multisystem vasculitic disease and the most often neurologic manifestation of BD is headache. Transcranial Doppler ultrasonography (TCD) is a test which used for evaluating the changes in blood flow velocity developed against visual stimulation. It is not well known TCD findings in BD patients who suffered from headache. <h3>Objectives</h3> To evaluate the frequency and the types of headache and to investigate cerebral reactivity by TCD in BD patients. <h3>Methods</h3> 113 patients with BD diagnosed based on diagnostic criteria of BD by ISG and 40 healthy individuals were included in the study. The patients with BD who had neurological involvement were not included to the study. Headache type was specified by a specialist neurologist according to International classification of headache disorders society criteria. TCD was applied to 62 patients with BD and 40 healthy individuals. TCD results were evaluated by a specialist neurologist. <h3>Results</h3> Headache was determined in 89 (78.8%) patients with BD. It was statistically significant compared to HC group (60%, p&lt;0.03). 48 of 89 BD patients had tension type of headache and 33 of them had migrainous type. No significant difference was found between BD patients and HC group in terms of cerebral reactivity by TCD. Low pulsatility index for both the right side and the left side were noted in BD patients suffering from headache compared to BD patients not having headache (p&lt;0.006, p&lt;0.003). No significant differences were found between tension type and migrainous type of headache in terms of TCD parameters. <h3>Conclusions</h3> Headache is common in BD patients, but cerebral reactivity is maintained. <h3>Acknowledgements</h3> None <h3>Disclosure of Interest</h3> None declared
Lenalidomide in Combination with CHOP in Patients with Angioimmunoblastic T-Cell Lymphoma (AITL): Final Analysis of Clinical and Molecular Data of a Phase 2 Lysa Study
Authors: Francois Lemonnier, Violaine Safar, Laurence de Leval, Anne Ségolène Cottereau, Laura Pelletier, Cyrielle Robe, Emmanuel Bachy, Guillaume Cartron, Marie-Pierre Moles, Audrey Letourneau, Edoardo Missiaglia, Alain Jacques Delmer, Reda Bouabdallah, Laurent Voillat, Marie Parrens, Olivier Casasnovas, Victoria Cacheux, Remy Gressin, Herve Tilly, Marie-Helene Delfau, Michel Meignan, Philippe Gaulard, Corinne Haioun
Journal: Blood (2018)
AB1451-HPR Burden among caregivers in rheumatoid arthritis – a pilot study
Authors: D. Buitrago-Garcia, I. Mendez, F Rodriguez, L. Villarreal, P. Santos-Moreno
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Rheumatoid arthritis (RA) is a chronic, inflammatory disease of the joints affecting more than 1% of global population, it is a long term condition that causes pain and disability.<sup>1</sup> Evidence had shown that most of the patients are moderately disabled, and about 10% of the patients suffered from severe disablement which brings the necessity of a caregiver to become the patient's companion due to its chronic disease.<sup>2</sup> The caregiving role can have an impact in the psychological and physical spheres of the caregiver's life.<sup>3</sup> <h3>Objectives</h3> The aim of this study was to explore demographical characteristics and caregiver burden through the Zarit Scale. <h3>Methods</h3> We conducted a cross sectional study in a meeting where caregivers in a rheumatoid arthritis specialised setting. We collected sociodemographic information, and applied the Zarit caregiver burden interview (ZBI) adapted to Spanish. The ZBI includes 22 questions which has 5 responses from 0 (never) to 4 (nearly always), where scores lower than 47 indicated little to no burden, 47 to 55 low burden and &gt;55 intense burden. We calculated means, and standard deviations for continuous variables and categorical variables were presented as rates. We categorised age of caregivers and compared to it to ZBI score, we used X<sup>2</sup> to perform bivariate analysis. <h3>Results</h3> 115 caregivers were included in the study, 63% were female and 37% were male. Mean age was 49 years±18 and 35% were single. Regarding educational level 44% had college degree, most of caregivers 30% had a full time job, and also 45% had other family members as their responsibility. See table 1. Zarit mean score was 44±14, additionally 71% had a score lower than 47. See table 1. Regarding age groups 35% of patients were older than 60 years and 15% of them were considered with intense burden disease, there was no statistical association between age and disease burden, see table 2. <h3>Conclusions</h3> Although a high proportion of caregivers reported to have not burden, it is important to develop strategies and activities in order to attend the necessities of the caregivers in order to reduce the overload of responsibilities among them. Also further research is needed in order to identify the risk factors or protector factors that can prevent disease burden in caregivers. <h3>References</h3> [1] Prothero L, Georgopoulou S, de Souza S. Patient involvement in the development of a handbook for moderate rheumatoid arthritis. 2016. [2] Jacobi CE, van den Berg B, Boshuizen HC, Rupp I, Dinant HJ, van den Bos GA. Dimension-specific burden of caregiving among partners of rheumatoid arthritis patients. Rheumatology (Oxford, England). 2003;42(10):1226–33. [3] Win KK, Chong MS, Ali N, Chan M, Lim WS. Burden among Family Caregivers of Dementia in the Oldest-Old: An Exploratory Study. Frontiers in medicine. 2017;4:205. <h3>Disclosure of Interest</h3> None declared
Postoperative Delirium—Amplifying the Confusion
Authors: Nirvik Pal
Journal: JAMA Surgery (2019)

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Allogeneic Stem Cell Transplantation (Allo-SCT) after Treatment with Programmed Cell Death-1 (PD-1) Checkpoint Inhibitors for Relapsed/Refractory Classic Hodgkin Lymphoma (R/R cHL) Is Associated with an Unprecedented Low Relapse Rate
Authors: Armando Santoro, Luca Castagna, Massimo Magagnoli, Stefania Bramanti, Jacopo Mariotti, Rita Mazza, Lucia Morello, Francesca Ricci, Barbara Sarina, Chiara De Philippis, Simona Sica, Marcello Rodari, Martina Sollini, Margarita Kirienko, Laura Giordano, Arturo Chiti, Paolo Corradini, Carmelo Carlo-Stella
Journal: Blood (2018)
FRI0599 The relevance of thermography and nailfold capillaroscopy in older patients with primary and secondary raynaud’s phenomenon
Authors: S. van Vugt, Y. Vreeburg, L. van Vugt, G. Schardijn
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Raynaud's phenomenon (RP) is defined as episodic attacks of artery and arteriole vasoconstriction. To differentiate between the benign RP (pRP) and the form associated with connective tissue diseases (sRP) the capillary morphology can be studied using nailfold capillaroscopy (nCS). However, abnormal morphology can also be caused due to age-related changes and has been described in patients with diabetes and cardiovascular disease. In addition, this technique cannot provide functional information. Thermal imaging (thermography; TG) is a non-invasive technique which enables quantification of cutaneous blood vessel function. In veterinary medicine, thermal imaging is applied for various clinical settings. A combined approach using both nCS and TG could improve the differentiation between pRP and sRP. <h3>Objectives</h3> The aim of this pilot study was to determine which technique (TG versus nCS) allows the best discrimination amongst older patients with pRP and sRP. <h3>Methods</h3> Thirty patients with RP (pRP, n=21; sRP, n=9) underwent nCS (Olympus SZ51) and TG (Flirr B 620). Nailfold morphologic features were measured and scored on capillary density, giant capillaries, ramification and hemorrages. The patients were divided into three categories: normal, slightly abnormal (slightly enlarged capillaries) and severely abnormal (destruction of capillary structure and hemorrages). TG of the hand was performed before, directly after and 10 min after a cold challenge test with cold manchets of 3°C. Rewarming and reperfusion were monitored and baseline images and rewarming curves were analysed. <h3>Results</h3> Capillary abnormalities with nCS were found in all patients with sRP (9/9) and in 52% (11/21) of patients with pRP. Out of 11 pRP patients with altered capillary morphology, 7 (63%) had a cardiovascular disease. TG demonstrated a lower average temperature at baseline in the pRP group compared to the sRP group (d 1,68°C, p&lt;0,01). In patients with pRP temperature decreased after cold induction (−2.34°C, p=0,01), whereas in sRP patient temperature stayed consistent (+0.07°C, p=0,46) (table 1). In both groups temperature increased ten minutes after cold induction (pRP +2.62°C, p&lt;0,01; sRP +1.57°C, p&lt;0,01). The gradient of the rewarming curve was significantly lower in patients with sRP compared to the pRP group (median 0.16 vs. 0.26 °C/min; p=0.015). <h3>Conclusions</h3> Nailfold capillaroscopy and thermography can reliably be used to measure microvascular damage and dysfunction. TG is better suitable to differentiate between older patients with pRP and sRP. Furthermore, in presence of cardiovascular disease, TG appears to be a more reliable technique than nCS for differentiating between patients with pRP and sRP. <h3>Disclosure of Interest</h3> None declared
AB1080 Neurological evaluation of childhood-onset cryopyrin-asscociated periodic syndromes-a preliminary report
Authors: H. Kilic, S. Sahin, C. Duman, A. Adrovic, K. Barut, O. Kasapcopur, S. Saltik
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> The cryopyrin-associated periodic syndrome (CAPS) is a treatable autoinflammatory disease that encompasses familial cold autoinflammatory syndrome(FCAS), Muckle–Wells syndrome(MWS), and chronic infantile, neurologic, cutaneous, and articular syndrome (CINCA), which are quite different in severity. Early diagnosis of CAPS and prompt initiation of IL-1 blockers have significant effect on the neurologic prognosis of CAPS. Although neurologic complications of CINCA are well-known, there are scarce date regarding neurologic features of milder phenotypes. <h3>Objectives</h3> We aimed to review the neurologic features in detail and summarise the other CAPS-related manifestations in 9 children. <h3>Methods</h3> All children with CAPS that have been followed-up from paediatric rheumatology outpatient clinic, were enrolled to the study. In addition to the neurologic examination, magnetic resonance imaging (MRI) of brain, electroencephalography, eye examination, hearing test and neuropsychiatric tests were done. Demographic, clinical features, genetic analysis and laboratory tests were noted from patient records and hospital database. <h3>Results</h3> The median age of the subjects was 6 years (range 2–14 years), with a female-to-male ratio 4/5. Most frequently noted neurologic clinical manifestations during the disease course were papilledema (3/9) and epilepsy (3/9), followed by neurodevelopmental delay (2/9), aseptic meningitis (2/9), upper motor neuron findings (2/9), ocular symptoms/signs (2/9), sensorineural hearing loss (1/9), optic atrophy (1/9) (table 1). MRI of the brain was abnormal in two patients. <h3>Conclusions</h3> Increased understanding and awareness of this rare but treatable syndrome among neurologists is essential, since the disease could manifest with neurologic manifestations such as seizure, papilledema, sensorineural deafness and aseptic meningitis. If remains untreated and recognised, this autoinflammatory syndrome could lead to significant morbidity and mortality. Hence, early treatment with anti-interleukin-1 therapy provides complete resolution of symptoms and, also prevent progression of neurologic findings when initiated in the late stage of the disease. <h3>Reference</h3> [1] Eroglu FK, Kasapcopur O, Beşbaş N, et al. Genetic and clinical features of cryopyrin-associated periodic syndromes in Turkish children. Clin Exp Rheumatol;34: 115–120. <h3>Disclosure of Interest</h3> None declared
A novel HBx genotype serves as a preoperative predictor and fails to activate the JAK1/STATs pathway in hepatocellular carcinoma
Authors: Qing-guo Xu, Sheng-xian Yuan, Qi-fei Tao, Jian Yu, Jie Cai, Yuan Yang, Xing-gang Guo, Kong-ying Lin, Jin-zhao Ma, De-shu Dai, Zhen-guang Wang, Fang-ming Gu, Ling-hao Zhao, Le-qun Li, Jing-feng Liu, Shu-han Sun, Yun-jin Zang, Hui Liu, Fu Yang, Wei-ping Zhou
Journal: Journal of Hepatology (2019)
Association of type 2 cytokines in severe rhinovirus bronchiolitis during infancy with risk of developing asthma: A multicenter prospective study
Authors: Kohei Hasegawa, Claire E. Hoptay, Brennan Harmon, Juan C. Celedón, Jonathan M. Mansbach, Pedro A. Piedra, Robert J. Freishtat, Carlos A. Camargo
Journal: Allergy (2019)

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Abstract 18092: The Relationship Between Patency of the Culprit Artery on Admission and Electrolyte Changes in Anterior STEMI - Analysis From the EMBRACE STEMI Clinical Trial
Authors: Serge Korjian, Yazan Daaboul, Robert A Kloner, Robert P Giugliano, W. D Weaver, Jim Carr, Nathan Michalak, Gerald Chi, Maria Villarreal, Anthony Gallo, Donald Szlosek, Purva Jain, Luke Rusowicz-Orazem, C. M Gibson
Journal: Circulation (2016)

Introduction: During STEMI, the activation of the renin-aldosterone-angiotensin system (RAAS) has a vasoconstrictive effect which may contribute to cardiac ischemia and the final infarct size. None...
MiR-454-3p-Mediated Wnt/β-catenin Signaling Antagonists Suppression Promotes Breast Cancer Metastasis
Authors: Liangliang Ren, Han Chen, Junwei Song, Xuhong Chen, Chun Lin, Xiaolan Zhang, Ning Hou, Jinyuan Pan, Zhongqiu Zhou, Lan Wang, Danping Huang, Jianan Yang, Yingying Liang, Jun Li, Hongbiao Huang, Lili Jiang
Journal: Theranostics (2019)

The Wnt/β-catenin pathway is constitutively active and promotes multiple tumor processes, including breast cancer metastasis. However, the underlying mechanism by which the Wnt/β-catenin pathway is constitutively activated in breast cancer metastasis remains unclear. Inhibition of Wnt antagonists is important for Wnt/β-catenin signaling activation, and post-transcriptional regulation of these antagonists by microRNAs (miRNAs) might be a possible mechanism underlying signaling activation. Regulation of nuclear pre-mRNA domain-containing 1A (RPRD1A) is a known inhibitor of cell growth and Wnt/β-catenin signaling activity, but the function and regulatory mechanism of RPRD1A in breast cancer have not been clarified. The aim of this study was to understand how regulators of the Wnt/β-catenin pathway may play a role in the metastasis of this cancer. Methods: RPRD1A expression and its association with multiple clinicopathological characteristics was analyzed immunohistochemically in human breast cancer specimens. miR-454-3p expression was analyzed using real-time PCR. RPRD1A or miR-454-3p knockdown and overexpression were used to determine the underlying mechanism of their functions in breast cancer cells. Xenografted tumor model, 3D invasive culture, cell migration and invasion assays and sphere formation assay were used to determine the biofunction of RPRD1A and miR-454-3p in breast cancer. Electrophoretic mobility shift assay (EMSA), luciferase reporter assay, and RNA immunoprecipitation (RIP) were performed to study the regulation and underlying mechanisms of RPRD1A and miR-454-3p expression and their correlation with the Wnt/β-catenin pathway in breast cancer. Results: The Wnt/β-catenin signaling antagonist RPRD1A was downregulated and its upstream regulator miR-454-3p was amplified and overexpressed in metastatic breast cancer, and both were correlated with overall and relapse-free survival in breast cancer patients. The suppression by miR-454-3p on RPRD1A was found to activate Wnt/β-catenin signaling, thereby promoting metastasis. Simultaneously, three other negative regulators of the Wnt/β-catenin pathway, namely, AXIN2, dickkopf WNT signaling pathway inhibitor (DKK) 3 and secreted frizzled related protein (SFRP) 1, were also found to be targets of miR-454-3p and were involved in the signaling activation. miR-454-3p was found to be involved in early metastatic processes and to promote the stemness of breast cancer cells and early relapse under both in vitro and in vivo conditions. Conclusions: The findings indicate that miR-454-3p-mediated suppression of Wnt/β-catenin antagonist RPRD1A, as well as AXIN2, DKK3 and SFRP1, sustains the constitutive activation of Wnt/β-catenin signaling; thus, miR-454-3p and RPRD1A might be potential diagnostic and therapeutic targets for breast cancer metastasis.