tbuckley/cabot-search huggingface dataset:
Effectiveness of trivalent seasonal influenza vaccine in preventing laboratory-confirmed influenza in primary care in the United Kingdom: 2012/13 end of season results
Authors: N Andrews, J McMenamin, H Durnall, J Ellis, A Lackenby, C Robertson, B von Wissmann, S Cottrell, B Smyth, C Moore, R Gunson, M Zambon, D Fleming, R Pebody
Journal: Eurosurveillance (2014)

The effectiveness of the 2012/13 trivalent seasonal influenza vaccine (TIV) was assessed using a test-negative case-control study of patients consulting primary care with influenza-like illness in the United Kingdom. Strain characterisation was undertaken on selected isolates. Vaccine effectiveness (VE) against confirmed influenza A(H3N2), A(H1N1) and B virus infection, adjusted for age, sex, surveillance scheme (i.e. setting) and month of sample collection was 26% (95% confidence interval (CI): -4 to 48), 73% (95% CI: 37 to 89) and 51% (95% CI: 34 to 63) respectively. There was an indication, although not significant, that VE declined by time since vaccination for influenza A(H3N2) (VE 50% within three months, 2% after three months, p=0.25). For influenza A(H3N2) this is the second season of low VE, contributing to the World Health Organization (WHO) recommendation that the 2013/14 influenza vaccine strain composition be changed to an A(H3N2) virus antigenically like cell-propagated prototype 2012/13 vaccine strain (A/Victoria/361/2011). The lower VE seen for type B is consistent with antigenic drift away from the 2012/13 vaccine strain. The majority of influenza B viruses analysed belong to the genetic clade 2 and were antigenically distinguishable from the 2012/13 vaccine virus B/Wisconsin/1/2010 clade 3. These findings supported the change to the WHO recommended influenza B vaccine component for 2013/14.
Peroral endoscopic myotomy with endoscopic fundoplication in a patient with idiopathic achalasia
Authors: Zaheer Nabi, Mohan Ramchandani, Santosh Darisetty, Rama Kotla, D. Nageshwar Reddy
Journal: Endoscopy (2019)

A 28-year-old man diagnosed with type II achalasia cardia underwent peroral endoscopic myotomy (POEM) via the anterior route (12 – 2 o'clock) using the standard technique. In this case we performed an endoscopic fundoplication in addition to the POEM procedure to prevent post-procedure reflux. Inoue et al. recently described the technique of POEM plus fundoplication [1].
Changes of serum glycome in patients suffering from ovarian cancer.
Authors: Veronique Blanchard, Karina Biskup, Elena Ioana Braicu, Jalid Sehouli, Rudolf Tauber, Markus Berger
Journal: Journal of Clinical Oncology (2012)

5047 Background: Protein glycosylation plays an important role in many biological processes. Most human serum proteins, with the exception of albumin, are glycosylated. Glycosylation is known to be altered with development of diseases such as cancer. In the case of ovarian cancer, tumor markers among them CA-125 that are clinically used are known to have poor specificity. In addition, they fail to detect the disease at an early stage. Therefore, better biomarkers are needed. The aim of the present research work is to identify new potential glycan biomarkers by analyzing the serum N-glycome of patients suffering from ovarian cancer Methods: Serum was collected from 67 patients as well as from 33 healthy age-matching women. N-glycans were released from 10 ul serum by PNGase F digestion, permethylated and subsequently analyzed by means of MALDI-TOF mass spectrometry. The SPSS software was used for the statistical analysis. Results: The N-glycome of patients was found to have more fucosylated structures, especially in tri- and tetraantennary sialylated glycans. The PCA analysis indicates that there are significant differences between the glycome of ovarian cancer patients in all stages of the disease and the glycome of healthy controls. We identified 14 potential structures that were divided in two categories, one of monofucosylated structures with high antennarity (sensitivity 94%, specificity 97%) and one containing high-mannose structures and an asialylated structures (sensitivity 97%, specificity 97%). Conclusions: Our study is the first trial to identify major differences between ovarian cancer sera and control sera, which could potentially be used in the future as biomarkers.
ENDOTHELIAL CELL-DERIVED EXTRACELLULAR VESICLES ELICIT NEUTROPHIL DEPLOYMENT FROM THE SPLEEN FOLLOWING ACUTE MYOCARDIAL INFARCTION
Authors: N Akbar, A Braithwaite, E Corr, G Koelwyn, C van Solingen, C Cochain, Saliba A-E., A Corbin, D Pezzolla, L Edgar, M Gunadasa-Rohling, A Banerjee, D Paget, C Lee, E Hogg, A Costin, R Dhaliwal, E Johnson, T Krausgruber, J Riepsaame, G Melling, M Shanmuganathan, C Bock, D Carter, K Channon, P Riley, I Udalova, K Moore, D Anthony, R Choudhury
Journal: BMJ (2020)
Abstract P348: Coronary Revascularization Procedure Mortality in Patients with Coexisting Pneumoconiosis: Outcomes after Coronary Artery Bypass Grafting and Angioplasty are Similar
Authors: Alexander V Sergeev
Journal: Circulation (2013)

Background: Diminished respiratory capacity due to excessive development of fibrous tissue in the lungs (pneumofibroisis) can pose a serious challenge for cardiovascular interventional therapy proc...
Stopping Mesalamine Therapy in Patients With Crohn’s Disease Starting Biologic Therapy Does Not Increase Risk of Adverse Outcomes
Authors: Ryan C. Ungaro, Berkeley N. Limketkai, Camilla Bjørn Jensen, Clara Yzet, Kristine H. Allin, Manasi Agrawal, Thomas Ullman, Johan Burisch, Tine Jess, Jean-Frederic Colombel
Journal: Clinical Gastroenterology and Hepatology (2019)
Evidence for a MAIT-17–high phenotype in children with severe asthma
Authors: Guillaume Lezmi, Rola Abou-Taam, Nicolas Garcelon, Céline Dietrich, François Machavoine, Christophe Delacourt, Karine Adel-Patient, Maria Leite-de-Moraes
Journal: Journal of Allergy and Clinical Immunology (2019)
New “sutureless” technique of ileostomy and colostomy
Authors: M. V. Phadke, L. H. Stocks, Y. G. Phadke
Journal: Journal of Clinical Oncology (2009)

e15132 Purpose: Purpose is to prevent infection and subsequent complications following construction of either an Ileostomy or a colostomy in an elective or emergency operation. Technique is based on delayed-primary wound healing and established principles of basic sciences. Methods: Discovered by serendipity in 1986 during an Ileostomy, procedure was used for all stomata. Stomal (proximal) end of bowel (small/large) was left obstructed with staples and brought out at proposed site of stoma. Mesenteric corner was brought above skin opening. A cone was formed by lifting anti-mesenteric corner of bowel as apex of the stoma by pulling anti-mesenteric surface of bowel. Length of the stoma was determined by thickness of the abdominal wall. Serosa was sutured circumferentially to a round opening in rectus sheath starting below the mesenteric corner leaving an arc to accommodate the mesenteric vessels. This neutralized pulling effect of peristalsis on mesenteric corner of stoma and protects mesentery under skin reducing the chances of thrombosis of mesenteric vessels. Stoma was covered in OR using an appliance with transparent pouch. Obstructed stoma prevented immediate contamination of stomal and main wounds by feces. Postoperatively, serosal surface was covered by angiogenesis, making the stomal wound refractory to infection. When peristalsis returned, bulging stoma was opened using electro-cautery as a bedside procedure. Mucosal cuff protrudes and everts on angiogenesis over a single layer of serosa. Cuff progresses with peristalsis and grafts over a bed of granulation tissue. Margins of cuff adhere with dermis of skin opening completing self-maturation of stoma. Absence of sutures reduced tissue trauma and eliminated foreign body reaction resulting in better wound healing. This new scientific technique was named “DELAYED-PRIMARY SELF-MATURATION (DPSM)”. Results: 63 Colostomies and 17 Ileostomies were performed using DPSM. Infection in stomal and/or main wound was prevented. Subsequent complications were prevented. Conclusions: DPSM prevents infection and complications associated with Ileostomy and Colostomy operations. It is technically easier and more scientific than a conventional stoma. This technique is recommended for all cases requiring end/loop, temporary/permanent types of intestinal stomas. No significant financial relationships to disclose.
Mischaracterization of Spaceflight-Associated Neuro-ocular Syndrome
Authors: Michael A. Williams, Jan Malm
Journal: JAMA Neurology (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Neurology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Neurology journal
Abstract #498: Analysis of histone modification in transcriptional attenuation of c-myc and cyclin D1 in response to sodium butyrate in colon cancer cells
Authors: Maria Daroqui, Leonard Augenlicht
Journal: Cancer Research (2009)

Sodium butyrate (NaB) is a short-chain fatty acid present at >20 mM in the human colon, principally as a result of dietary fiber fermentation. While NaB is the primary energy source of colonocytes, it induces cell cycle arrest, differentiation and apoptosis in colon cancer cells, through mechanisms not completely understood. We have reported that although butyrate enhances s-catenin-Tcf signaling, which positively regulates c-myc (Bordonaro et al, Cancer Res. 1999; Wilson et al, Cancer Res. 2002), it also induces c-myc transcriptional attenuation, which consists of a post-initiation pause or blockage in transcription. We recently demonstrated a similar effect of NaB at the cyclin D1 locus, another key regulator of cell proliferation that is also a target of s-catenin-Tcf signaling (Maier et al, J. Cell. Physiol. 2008). To define better the molecular mechanisms involved in NaB-induced transcriptional attenuation, the c-myc and cyclin D1 genes were analyzed in SW837 human colorectal adenocarcinoma cells utilizing a \#8220;tiling\#8221; microarray designed with ~50 nt long probes spanning the entire RefSeq. We found that the level of expression of c-myc and cyclin D1 transcripts were markedly decreased after 6 and 8 h treatment with NaB (5 mM), and that this downregulation occurred at the very 5\#8217; end of the coding sequence (e.g.: starting at nt 80 of c-myc RefSeq), indicating that butyrate control indeed occurs early in the transcriptional process. Butyrate also alters chromatin structure and function through its inhibition of histone deacetylase activity (HDACi), resulting in histone hyperacetylation. Chromatin immunoprecipitation (ChIP) followed by qRT-PCR, revealed that the level of histone H3 acetylation (lysines 9 and 14) at both the c-myc and cyclin D1 loci, was decreased ~70% after butyrate treatment, while it increased over 2-fold in the p21 gene, one of the major targets of NaB-induced expression. Since H3 lysine 9 (K9) acetylation occurs synchronously with K4 methylation as marks of active chromatin, we investigated H3 methylation status in response to butyrate. While it was induced at the p21 locus, no increase in trimethylated K4 was observed in either the c-myc or cyclin D1 genes. Moreover, similar results were found in H3 trimethylated K36, a mark of transcriptional elongation, in response to NaB. Taken together, our data indicate that butyrate exerts a negative control of transcriptional activity at the c-myc and cyclin D1 loci, potentially principally through attenuation of elongation.  Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 498.
Mitotic catastrophe induced in cells treated with SDX-105 (Treanda™)
Authors: Christina Niemeyer, Heather Bendall, Brandi Bailey, Jack Reifert, Lorenzo M. Leoni
Journal: Cancer Research (2005)

2291   SDX-105 (Treanda™, bendamustine), an alkylating agent with a distinct mechanism of action, is active in NHL and CLL patients refractory to traditional DNA-damaging agents. Key features of SDX-105 activity include its lack of cross-resistance with other DNA-alkylating agents and the unique gene expression profile it induces when compared to other 2-chloroethylamine alkylating agents, such as chlorambucil and cyclophosphamide. The G 2 /M checkpoint regulators Polo-like kinase 1 (PLK-1) and Aurora A kinase (AurkA) are down-regulated in SU-DHL-1, SU-DHL-9 and Daudi cells after 8 hours of exposure to clinically achievable concentrations of SDX-105. Multi-drug resistant MCF-7/ADR cells and p53 deficient RKO-E6 colon adenocarcinoma cells exposed for two or three days to either 50 μM SDX-105 alone or 50 μM SDX-105 and 20 μM pan-caspase inhibitor zVAD-fmk were evaluated for induction of mitotic catastrophe. Microscopic analysis of nuclear morphology using DNA-staining with DAPI revealed an increased incidence of chromatin condensation and micronucleation in SDX-105-treated cells, even in presence of zVAD-fmk, hallmarks of mitotic catastrophe. The concomitant down-regulation of mitotic checkpoint genes and the induction of DNA damage by SDX-105 may amplify the cytotoxicity of SDX-105 through a necrotic cell death pathway and hence its activity in tumor cells where classical apoptotic pathways are inhibited. This may explain, at least in part, the potent anti-tumor activity of SDX-105 in tumor cells refractory to conventional DNA-damaging agents. Additional studies are ongoing to further elaborate the role of mitotic catastrophe in SDX-105’s mechanism of action. The capacity to induce mitotic catastrophe may explain, at least in part, the anti-tumor activity of SDX-105 in chemotherapy relapsed and resistant patients.
Abstract 2709: Saline Injection Test (SIT) May Provide Inaccurate Evaluation of Mitral-Left Ventricular (LV) Dimension During Mitral Valve Plasty (MVP)
Authors: Michihito Nonaka, Akira Marui, Takeshi Shimamoto, Masahira Fukuoka, Shinji Masuyama, Tadashi Ikeda, Masashi Komeda
Journal: Circulation (2006)

Introduction : SIT during MVP has been popular; however, geometric discrepancies of mitral-LV complex between saline-injected LV and beating LV may result in incomplete MVP. Thus, we compared 3 dim...
Imatinib-loaded gold nanoparticles inhibit proliferation of fibroblasts and macrophages from systemic sclerosis patients and ameliorate experimental bleomycin-induced lung fibrosis
Authors: Veronica Codullo, Emanuela Cova, Laura Pandolfi, Silvia Breda, Monica Morosini, Vanessa Frangipane, Manuela Malatesta, Laura Calderan, Maddalena Cagnone, Chiara Pacini, Lorenzo Cavagna, Helios Recalde, Jörg H.W. Distler, Marco Giustra, Davide Prosperi, Miriam Colombo, Federica Meloni, Carlomaurizio Montecucco
Journal: Journal of Controlled Release (2019)
NADPH oxidases in the differentiation of endothelial cells
Authors: Fabian Hahner, Franziska Moll, Katrin Schröder
Journal: Cardiovascular Research (2019)

The differentiation of stem cells into endothelial cells involves the modulation of highly interconnected metabolic and epigenetic processes. Therefore, the differentiation of endothelial cells is a tightly controlled process, which is adjusted at multiple levels, meaning that even the smallest variation can result in major consequences. Reactive oxygen species (ROS) represent a group of second messengers that can interfere with both metabolic and epigenetic processes. Besides their generation by mitochondria, ROS are produced in a controlled manner by the family of NADPH oxidases. The different members of the NADPH oxidase family produce superoxide anions or hydrogen peroxide. Due to the specific sub-cellular localization of the different NADPH oxidases, ROS are produced at diverse sites in the cell, such as the plasma membrane or the endoplasmic reticulum. Once produced, ROS interfere with proteins, lipids, and DNA to modulate intracellular signal cascades. Accordingly, ROS represent a group of readily available and specifically localized modulators of the highly sophisticated signalling network that eventually leads to the differentiation of stem cells into endothelial cells. This review focuses on the role of NADPH oxidases in the differentiation of stem cells into endothelial cells.
Methylation status of BCRP detected by methylation-specific PCR is inversely correlated with its expression and drug resistance in vitro and in patients with cancers
Authors: Hirofumi Nakano, Hiroshi Soda, Masaaki Fukuda, Shigeru Kohno, Kazuhiro Tsukamoto
Journal: Cancer Research (2006)

1  Breast cancer resistant protein (BCRP) functions as a drug efflux transporter that mediates drug resistance. Topoisomerase I inhibitors including SN-38 (an active metabolite of irinotecan) are substrates effluxed by BCRP. The underlying mechanisms for overexpression of BCRP during acquisition of drug resistance remain unclear. The aims of this study are to examine a correlation of altered methylation status in the promoter region of BCRP with BCRP expression and drug resistance in lung and colorectal cancers, and to establish a methylation-specific PCR (MSP) method for BCRP. Treatment of non-BCRP-expressing PC-6 cells with DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine, induced BCRP re-expression at the mRNA and protein levels. Bisulfite sequencing analysis and subsequent comparisons between bisulfite-modified and unmodified DNA sequences at CpG sites in the promoter region of BCRP (spanning from nt -1381 to +261) revealed that both alleles at all 89 CpG sites were completely methylated in PC-6, while those at 79 CpG sites (at nt -720 to +261) in BCRP-expressing cells of PC-6/SN2-5H were unmethylated. These results indicate a correlation between demethylation of the promoter region of BCRP and BCRP re-expression, and suggest a mechanism for SN-38 drug resistance. Analysis using MSP we developed also revealed an inverse correlation between methylation status of the promoter region of BCRP and BCRP mRNA expression in several lung and colorectal cancers. Examination of methylation status of the promoter region of BCRP using MSP before chemotherapy may provide a new biomarker for predicting drug resistance and responsibility.
Biomaterial and biomechanical considerations to prevent risks in implant therapy
Authors: Estevam A. Bonfante, Ryo Jimbo, Lukasz Witek, Nick Tovar, Rodrigo Neiva, Andrea Torroni, Paulo G. Coelho
Journal: Periodontology 2000 (2019)

Abstract This paper is aimed to present a biomaterials perspective in implant therapy that fosters improved bone response and long‐term biomechanical competence from surgical instrumentation to final prosthetic rehabilitation. Strategies to develop implant surface texturing will be presented and their role as an ad hoc treatment discussed in light of the interplay between surgical instrumentation and implant macrogeometric configuration. Evidence from human retrieved implants in service for several years and from in vivo studies will be used to show how the interplay between surgical instrumentation and implant macrogeometry design affect osseointegration healing pathways, and bone morphologic and long‐term mechanical properties. Also, the planning of implant‐supported prosthetic rehabilitations targeted at long‐term performance will be appraised from a standpoint where personal preferences (eg, cementing or screwing a prosthesis) can very often fail to deliver the best patient care. Lastly, the acknowledgement that every rehabilitation will have its strength degraded over time once in function will be highlighted, since the potential occurrence of even minor failures is rarely presented to patients prior to treatment.
3D Bioprinting: The Emergence of Programmable Biodesign
Authors: Sara Correia Carreira, Runa Begum, Adam W. Perriman
Journal: Advanced Healthcare Materials (2019)

Abstract Until recently, bioprinting was largely limited to highly interdisciplinary research teams, as the process requires significant input from specialists in the fields of materials science, engineering, and cell biology. With the advent of commercially available high‐performance bioprinters, the field has become accessible to a wider range of research groups, who can now buy the hardware off the shelf instead of having to build it from scratch. As a result, bioprinting has rapidly expanded to address a wide array of research foci, which include organotypic in vitro models, complex engineered tissues, and even bioprinted microbial systems. Moreover, in the early days, the range of suitable bioinks was limited. Now, there is a plethora of viable options to suit many cell phenotypes. This rapidly evolving dynamic environment creates endless opportunities for scientists to design and construct highly complex biological systems. However, this scientific diversity presents its own set of challenges, such as defining standardized protocols for characterizing bioprinted structures, which is essential for eventual organ replacement. In this progress report, the current state‐of‐the‐art in the field of bioprinting is discussed, with a special emphasis on recent hardware developments, bioprinting for regenerative medicine, and late‐breaking nontraditional topics.
Eigenrank by committee: Von-Neumann entropy based data subset selection and failure prediction for deep learning based medical image segmentation
Authors: Bilwaj Gaonkar, Joel Beckett, Mark Attiah, Christine Ahn, Matthew Edwards, Bayard Wilson, Azim Laiwalla, Banafsheh Salehi, Bryan Yoo, Alex A.T. Bui, Luke Macyszyn
Journal: Medical Image Analysis (2020)
Strategies to Adjust Positive End-Expiratory Pressure in Patients With ARDS—Reply
Authors: Jeremy R. Beitler, Daniel Talmor
Journal: JAMA (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal