tbuckley/cabot-search huggingface dataset:
Genetic characterization of an H5N6 avian influenza virus from chickens in Guangdong, China
Authors: Jing Yang, Yong Li, Jinyue Guo, Kaijian Luo, Hai Yu, Yao Chen, Anqi Li, Sheng Yuan, Saeed El-Ashram, Shujian Huang, Feng Wen
Journal: Journal of Infection (2020)
TCT CONNECT-325 Usefulness of Optical Coherence Tomography in Myocardial Infarction With Nonobstructive Coronary Arteries
Authors: Caterina Mas-Lladó, Jaume Maristany, Josep Gomez Lara, Marcos Pascual, Alameda Maria Del Mar, Alfredo Gomez, Vicente Peral-Disdier
Journal: Journal of the American College of Cardiology (2020)
Activatable Zymography Probes Enable <i>In Situ</i> Localization of Protease Dysregulation in Cancer
Authors: Ava P. Soleimany, Jesse D. Kirkpatrick, Susan Su, Jaideep S. Dudani, Qian Zhong, Ahmet Bekdemir, Sangeeta N. Bhatia
Journal: Cancer Research (2020)

Abstract Recent years have seen the emergence of conditionally activated diagnostics and therapeutics that leverage protease-cleavable peptide linkers to enhance their specificity for cancer. However, due to a lack of methods to measure and localize protease activity directly within the tissue microenvironment, the design of protease-activated agents has been necessarily empirical, yielding suboptimal results when translated to patients. To address the need for spatially resolved protease activity profiling in cancer, we developed a new class of in situ probes that can be applied to fresh-frozen tissue sections in a manner analogous to immunofluorescence staining. These activatable zymography probes (AZP) detected dysregulated protease activity in human prostate cancer biopsy samples, enabling disease classification. AZPs were leveraged within a generalizable framework to design conditional cancer diagnostics and therapeutics and showcased in the Hi-Myc mouse model of prostate cancer, which models features of early pathogenesis. Multiplexed screening against barcoded substrates yielded a peptide, S16, that was robustly and specifically cleaved by tumor-associated metalloproteinases in the Hi-Myc model. In situ labeling with an AZP incorporating S16 revealed a potential role of metalloproteinase dysregulation in proliferative, premalignant Hi-Myc prostatic glands. Systemic administration of an in vivo imaging probe incorporating S16 perfectly classified diseased and healthy prostates, supporting the relevance of ex vivo activity assays to in vivo translation. We envision AZPs will enable new insights into the biology of protease dysregulation in cancer and accelerate the development of conditional diagnostics and therapeutics for multiple cancer types. Significance: Visualization of protease activity within the native tissue context using AZPs provides new biological insights into protease dysregulation in cancer and guides the design of conditional diagnostics and therapeutics.
<i>Notes from the Field:</i> Characteristics of E-cigarette, or Vaping, Products Confiscated in Public High Schools in California and North Carolina — March and May 2019
Authors: Mays Shamout, Lauren Tanz, Carolyn Herzig, Lisa P. Oakley, Corey M. Peak, Amy Heinzerling, Marisa Hast, Eileen McGowan, Rebecca J. Williams, Catherine Hess, Chunxia Wang, Sarah Planche, Sally Herndon, Jim Martin, Susan M. Kansagra, Maeh Al-Shawaf, Paul Melstrom, Kristy Marynak, Michael A. Tynan, Israel T. Agaku, Brian A. King
Journal: MMWR Morbidity and Mortality Weekly Report (2020)
The number of successful injections associates with improved clinical effect in intralymphatic immunotherapy
Authors: Søren Helbo Skaarup, Ole Graumann, Johannes Schmid, Anne‐Sofie Bjerrum, Tina Skjold, Hans Jürgen Hoffmann
Journal: Allergy (2020)

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Plasma Proteomics Identify Biomarkers and Pathogenesis of COVID-19
Authors: Ting Shu, Wanshan Ning, Di Wu, Jiqian Xu, Qiangqiang Han, Muhan Huang, Xiaojing Zou, Qingyu Yang, Yang Yuan, Yuanyuan Bie, Shangwen Pan, Jingfang Mu, Yang Han, Xiaobo Yang, Hong Zhou, Ruiting Li, Yujie Ren, Xi Chen, Shanglong Yao, Yang Qiu, Ding-Yu Zhang, Yu Xue, You Shang, Xi Zhou
Journal: Immunity (2020)
Introduction of standardised packaging and availability of illicit cigarettes: a difference-in-difference analysis of European Union survey data 2015–2018
Authors: Anthony A Laverty, Christopher Millett, Nicholas S Hopkinson, Filippos T Filippidis
Journal: Thorax (2020)

Standardised packaging of tobacco products is intended to reduce the appeal of smoking, but the tobacco industry claims this increases illicit trade. We examined the percentage of people reporting being offered illicit cigarettes before and after full implementation of standardised packaging in the UK, Ireland and France and compared this to other European Union countries. Reported ever illicit cigarette exposure fell from 19.8% to 18.1% between 2015 and 2018 in the three countries fully implementing the policy, and from 19.6% to 17.0% in control countries (p for difference=0.320). Standardised packaging does not appear to increase the availability of illicit cigarettes.
TCT CONNECT-473 Reproducibility of Cusp Overlap Technique to Reduce Permanent Pacemaker Implantation With Evolut: The Latin American Experience
Authors: Hemal Gada, Amit Vora, Oscar Millan-Iturbe, Matias Sztejfman, Rogerio Tumelero, Luis Gutierrez
Journal: Journal of the American College of Cardiology (2020)
The Association of Aging Biomarkers, Interstitial Lung Abnormalities, and Mortality
Authors: Jason L. Sanders, Rachel K. Putman, Josée Dupuis, Hanfei Xu, Joanne M. Murabito, Tetsuro Araki, Mizuki Nishino, Emelia J. Benjamin, Daniel Levy, Vasan S. Ramachandran, George R. Washko, Jeffrey L. Curtis, Christine M. Freeman, Russell P. Bowler, Hiroto Hatabu, George T. O’Connor, Gary M. Hunninghake
Journal: American Journal of Respiratory and Critical Care Medicine (2020)

The association between aging and idiopathic pulmonary fibrosis has been established. The associations between aging-related biomarkers and interstitial lung abnormalities (ILA) have not been comprehensively evaluated.
Use of exhaled breath condensate (EBC) in the diagnosis of SARS-COV-2 (COVID-19)
Authors: Daniel J Ryan, Sinead Toomey, Stephen F Madden, Michelle Casey, Oscar S Breathnach, Patrick G Morris, Liam Grogan, Peter Branagan, Richard W Costello, Eoghan De Barra, Killian Hurley, Cedric Gunaratnam, Noel G McElvaney, Michael Emmet OBrien, Imran Sulaiman, Ross K Morgan, Bryan T Hennessy
Journal: Thorax (2020)

False negatives from nasopharyngeal swabs (NPS) using reverse transcriptase PCR (RT-PCR) in SARS-CoV-2 are high. Exhaled breath condensate (EBC) contains lower respiratory droplets that may improve detection. We performed EBC RT-PCR for SARS-CoV-2 genes (E, S, N, ORF1ab) on NPS-positive (n=16) and NPS-negative/clinically positive COVID-19 patients (n=15) using two commercial assays. EBC detected SARS-CoV-2 in 93.5% (29/31) using the four genes. Pre-SARS-CoV-2 era controls (n=14) were negative. EBC was positive in NPS negative/clinically positive patients in 66.6% (10/15) using the identical E and S (E/S) gene assay used for NPS, 73.3% (11/15) using the N/ORF1ab assay and 14/15 (93.3%) combined.
Hepatocyte Nuclear Factor 4α Prevents the Steatosis‐to‐NASH Progression by Regulating p53 and Bile Acid Signaling (in mice)
Authors: Yanyong Xu, Yingdong Zhu, Shuwei Hu, Yang Xu, Diane Stroup, Xiaoli Pan, Fathima Cassim Bawa, Shaoru Chen, Raja Gopoju, Liya Yin, Yanqiao Zhang
Journal: Hepatology (2020)

Hepatocyte nuclear factor 4α (HNF4α) is highly enriched in the liver, but its role in the progression of nonalcoholic liver steatosis (NAFL) to NASH has not been elucidated. In this study, we investigated the effect of gain or loss of HNF4α function on the development and progression of NAFLD in mice.Overexpression of human HNF4α protected against high-fat/cholesterol/fructose (HFCF) diet-induced steatohepatitis, whereas loss of Hnf4α had opposite effects. HNF4α prevented hepatic triglyceride accumulation by promoting hepatic triglyceride lipolysis, fatty acid oxidation, and VLDL secretion. Furthermore, HNF4α suppressed the progression of NAFL to NASH. Overexpression of human HNF4α inhibited HFCF diet-induced steatohepatitis in control mice but not in hepatocyte-specific p53-/- mice. In HFCF diet-fed mice lacking hepatic Hnf4α, recapitulation of hepatic expression of HNF4α targets cholesterol 7α-hydroxylase and sterol 12α-hydroxylase and normalized hepatic triglyceride levels and attenuated steatohepatitis.The current study indicates that HNF4α protects against diet-induced development and progression of NAFLD by coordinating the regulation of lipolytic, p53, and bile acid signaling pathways. Targeting hepatic HNF4α may be useful for treatment of NASH.
TCT CONNECT-397 Comparison of Prognostic Value of Fractional Flow Reserve in Deferred and Functionally Optimized PCI Patients With Stable Coronary Artery Disease
Authors: Rimsha Hasan, Mohammed Eid Madmani, Malek Al-Hawwas, Mohamed Ayan, Aisha Siraj, Negar Salehi, Rahil Rafeedheen, Srikanth Vallurupalli, Mallik Edupuganti, Barry Uretsky, Shiv Kumar Agarwal
Journal: Journal of the American College of Cardiology (2020)
Substance Use Disorder Linked to Higher COVID-19 Risk
Authors: Rita Rubin
Journal: JAMA (2020)

People with substance use disorder (SUD), particularly those with opioid use disorder (OUD) and Black individuals, are at an increased risk of coronavirus disease 2019 (COVID-19), according to a recent study coauthored by Nora Volkow, MD, director of the National Institute on Drug Abuse (NIDA).
Abstract 14208: A Novel Augmentation Device for Ventilation Utilizing the Bag Valve Mask in EMS and Resource Limited Settings
Authors: Rohith Malya, Carolina De Santiago, Natalie Dickman, Madison Nasteff, Tim Nonet, Aravind Sundaramraj, Karen Vasquez, Sabia Abidi, Maria Oden
Journal: Circulation (2019)

Introduction: EMS and Low-and-middle income countries (LMICs) do not have ubiquitous access to mechanical ventilators which are limited by durability, cost, training, manpower, and power consumptio...
TCT CONNECT-290 Evaluating the Long-Term Clinical Outcomes of Different Treatment Modalities for Intrastent Chronic Total Occlusion
Authors: Sandeep Basavarajaiah, Satoru Mitomo, Sunao Nakamura, Sampath Athukorala, George Pulikal, Gurbir Bhatia, Richard Watkin
Journal: Journal of the American College of Cardiology (2020)
The Missing Piece — SARS-CoV-2 Testing and School Reopening
Authors: Yasmin Rafiei, Michelle M. Mello
Journal: New England Journal of Medicine (2020)

SARS-CoV-2 Testing and School Reopening Most U.S. school reopening plans focus on screening for Covid-19 symptoms. But because an estimated 40% of Covid-19 cases are asymptomatic and 50% of transmi...
OP0139 Regulation of cd154 in systemic lupus erythematosus
Authors: RQ Cron, A Genin, M Brunner
Journal: Annals of the Rheumatic Diseases (2001)

<h3></h3> CD40-ligand (CD154) is expressed on the surface of activated CD4 T cells and as a soluble cytokine. CD154 is a critical regulator of the immune response via its interaction with CD40 on various cell types, and, therefore, it is not surprising that its expression is tightly controlled. Current reports have described abnormal expression (increased and prolonged levels) of CD154 in adult patients with systemic lupus erythematosus (SLE), similar to observations in lupus-prone mice. We have recently confirmed these findings in paediatric SLE patients. Normally, like several tumour necrosis factor cytokine family members, CD154 expression is primarily controlled at the level of gene transcription and mRNA stability. Moreover, regulatory defects in intracellular signalling pathways that may effect cytokine mRNA levels have recently been described in CD4 T cells from patients with SLE. Using real-time quantitative PCR we have found that CD154 mRNA levels are increased in SLE patients versus matched controls. Preliminary results from nuclear run-on assays also suggest that increased CD154 transcription plays a role in CD154 hyper-expression in SLE. The CD154 <i>cis</i>-element (s) responsible for this increase in transcription is unknown. Although we had previously identified a relatively weak proximal 5′ flank transcriptional promoter, very little is known about the <i>cis-</i> and <i>trans</i>-regulatory elements that contribute to CD154 gene expression. We have recently identified a NFκB-responsive transcriptional enhancer element located within and just downstream of the 3′ CD154 untranslated region. Moreover, using nuclei from primary human CD4 T cells, we have identified a novel DNase I hypersensitive site located approximately 1 kb upstream of the proximal promoter. This novel site is flanked by several potential T cell transcription factor binding sites and, thus, may function as a transcriptional enhancer. We are currently comparing the transcriptional activities of these various CD154 <i>cis</i>-regulatory elements in T cells from SLE patients and controls in order to identify region (s) that contribute to enhanced CD154 expression in SLE. Ultimately, we hope to be able to target the <i>cis</i>- or <i>trans</i>-regulatory elements that lead to the abnormal expression of CD154 in SLE and various other autoimmune disorders.
Severe acute pancreatitis caused by endoscopic ultrasonography-guided fine-needle biopsy of a pancreatic solid pseudopapillary neoplasm
Authors: Su Luo, De-feng Li, Liliangzi Guo, Lu Bai, Li-sheng Wang, Jun Yao
Journal: Endoscopy (2020)

A 33-year-old man was admitted to our hospital for investigation of a mass in the pancreatic tail found on computed tomography (CT) scan 3 months previously. Endoscopic ultrasonography (EUS) revealed a 3.0 × 3.1-cm hypoechoic lesion in the tail of the pancreas, with no wall nodules, separation, or communication with the pancreatic duct ([Fig. 1]). EUS-guided fine-needle biopsy (EUS-FNB) was performed with a 22G ultrasound biopsy needle with a side hole (ECHO-HD-22-C; Cook Echotip ProCore). We obtained sufficient tissue with no cystic fluid by puncturing the lesion three times, each time under 5-mL negative pressure with 15 to 20 insertions ([Video 1]). The mass was eventually diagnosed as a solid pseudopapillary tumor on pathology with immunohistochemical staining ([Fig. 2]).
Val Curtis: public health researcher and activist
Authors: Robert Aunger, Oliver Cumming
Journal: BMJ (2020)

Credit: Aunger, 2020

Val Curtis served as the director of the Environmental Health Group at the London School of Hygiene and Tropical Medicine where she built a reputation as a world class researcher and passionate advocate for safe water, sanitation, and hygiene (WASH), leading a large and dynamic group of researchers.

Many people learnt of her illness through an article published in the Guardian .1 In characteristic fashion, she saw in her personal tragedy an opportunity to highlight a public health problem—in this case, the human impact of fiscal austerity on the NHS. Even in her final weeks, and despite her ill health and associated discomfort, she was an active member of SPI-B—the UK government’s Independent Scientific Pandemic Insights Group on Behaviours for covid-19—and continued to serve as an adviser to the Indian and Tanzanian governments on their national sanitation campaigns, which she had helped design.

Her multidisciplinary background, spanning engineering, public health, and evolutionary anthropology, made her unusual in the department of disease control at the London School of Hygiene and Tropical Medicine. …
CS<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" altimg="si1.svg"><mml:msup><mml:mrow/><mml:mn>2</mml:mn></mml:msup></mml:math>-Net: Deep learning segmentation of curvilinear structures in medical imaging
Authors: Lei Mou, Yitian Zhao, Huazhu Fu, Yonghuai Liu, Jun Cheng, Yalin Zheng, Pan Su, Jianlong Yang, Li Chen, Alejandro F. Frangi, Masahiro Akiba, Jiang Liu
Journal: Medical Image Analysis (2020)
TCT CONNECT-67 Antithrombotic Treatment Strategies in Patients Older Than 75 Years With Atrial Fibrillation After Percutaneous Coronary Intervention With Drug-Eluting Stents: The PACO-PCI Registry
Authors: Jose De la Torre Hernandez, José Luis Ferreiro, Ramón López Palop, Soledad Ojeda, David Marti, Pablo Avanzas, David Serrano, Alejandro Diego Nieto, Miren Telleria, Ignacio Amat-Santos, Jose Antonio Linares Vicente, Belen Cid Alvarez, Iñigo Lozano, Cristobal Urbano, Marcos Garcia-Guimaraes, Armando Perez de Prado, Felipe Hernandez Hernandez, Mario Sadaba, Angel Sanchez Recalde, Eduardo Pinar Bermudez, Rafael Gonzalez Manzanares, Ricardo Concepcion, Luis Arboine, Celia Garilleti
Journal: Journal of the American College of Cardiology (2020)
TCT CONNECT-100 A Novel Method of Assessing Commissural Alignment for the Sapien 3 Transcatheter Aortic Valve
Authors: Nikolaos Spilias, Nabil Sabbak, Grant Reed, Rishi Puri, James Yun, Shinya Unai, Amar Krishnaswamy, Serge Harb, Samir Kapadia
Journal: Journal of the American College of Cardiology (2020)
Author Correction: Rapid SARS-CoV-2 whole-genome sequencing and analysis for informed public health decision-making in the Netherlands
Authors: Bas B. Oude Munnink, David F. Nieuwenhuijse, Mart Stein, Áine O’Toole, Manon Haverkate, Madelief Mollers, Sandra K. Kamga, Claudia Schapendonk, Mark Pronk, Pascal Lexmond, Anne van der Linden, Theo Bestebroer, Irina Chestakova, Ronald J. Overmars, Stefan van Nieuwkoop, Richard Molenkamp, Annemiek A. van der Eijk, Corine GeurtsvanKessel, Harry Vennema, Adam Meijer, Andrew Rambaut, Jaap van Dissel, Reina S. Sikkema, Aura Timen, Marion Koopmans, G. J. A. P. M. Oudehuis, Janke Schinkel, Jan Kluytmans, Marjolein Kluytmans-van den Bergh, Wouter van den Bijllaardt, Robbert G. Berntvelsen, Miranda M. L. van Rijen, Peter Schneeberger, Suzan Pas, Bram M. Diederen, Anneke M. C. Bergmans, P. A. Verspui van der Eijk, Jaco J. Verweij, Anton G. N. Buiting, Roel Streefkerk, A. P. Aldenkamp, P. de Man, J. G. M. Koelemal, D. Ong, S. Paltansing, N. Veassen, Jacqueline Sleven, Leendert Bakker, Heinrich Brockhoff, Ariene Rietveld, Fred Slijkerman Megelink, James Cohen Stuart, Anne de Vries, Wil van der Reijden, A. Ros, Esther Lodder, Ellen Verspui-van der Eijk, Inge Huijskens, E. M. Kraan, M. P. M. van der Linden, S. B. Debast, N. Al Naiemi, A. C. M. Kroes, Marjolein Damen, Sander Dinant, Sybren Lekkerkerk, Oscar Pontesilli, Pieter Smit, Carla van Tienen, P. C. R. Godschalk, Jorien van Pelt, Alewijn Ott, Charlie van der Weijden, Heiman Wertheim, Janette Rahamat-Langendoen, Johan Reimerink, Rogier Bodewes, Erwin Duizer, Bas van der Veer, Chantal Reusken, Suzanne Lutgens, Peter Schneeberger, Mirjam Hermans, P. Wever, A. Leenders, Henriette ter Waarbeek, Christian Hoebe
Journal: Nature Medicine (2020)

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Tumor Fibroblast–Derived FGF2 Regulates Expression of SPRY1 in Esophageal Tumor–Infiltrating T Cells and Plays a Role in T-cell Exhaustion
Authors: Qing-yun Chen, Yi-ni Li, Xin-yue Wang, Xu Zhang, Yi Hu, Lei Li, Da-qin Suo, Ke Ni, Zhuo Li, Jia-rong Zhan, Ting-ting Zeng, Ying-hui Zhu, Yan Li, Li-jia Ma, Xin-Yuan Guan
Journal: Cancer Research (2020)

T-cell exhaustion was initially identified in chronic infection in mice and was subsequently described in humans with cancer. Although the distinct signature of exhausted T (TEX) cells in cancer has been well investigated, the molecular mechanism of T-cell exhaustion in cancer is not fully understood. Using single-cell RNA sequencing, we report here that TEX cells in esophageal cancer are more heterogeneous than previously clarified. Sprouty RTK signaling antagonist 1 (SPRY1) was notably enriched in two subsets of exhausted CD8+ T cells. When overexpressed, SPRY1 impaired T-cell activation by interacting with CBL, a negative regulator of ZAP-70 tyrosine phosphorylation. Data from the Tumor Immune Estimation Resource revealed a strong correlation between FGF2 and SPRY1 expression in esophageal cancer. High expression of FGF2 was evident in fibroblasts from esophageal cancer tissue and correlated with poor overall survival. In vitro administration of FGF2 significantly upregulated expression of SPRY1 in CD8+ T cells and attenuated T-cell receptor-triggered CD8+ T-cell activation. A mouse tumor model confirmed that overexpression of FGF2 in fibroblasts significantly upregulated SPRY1 expression in TEX cells, impaired T-cell cytotoxic activity, and promoted tumor growth. Thus, these findings identify FGF2 as an important regulator of SPRY1 expression involved in establishing the dysfunctional state of CD8+ T cells in esophageal cancer. SIGNIFICANCE: These findings reveal FGF2 as an important regulator of SPRY1 expression involved in establishing the dysfunctional state of CD8+ T cells and suggest that inhibition of FGF2 has potential clinical value in ESCC. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/80/24/5583/F1.large.jpg.
COVID ageism as a public mental health concern
Authors: Eva-Marie Kessler, Catherine E Bowen
Journal: The Lancet Healthy Longevity (2020)

Whenever older people (aged 65 years or older) are mentioned in relation to COVID-19, it is nearly always as a risk group. The frequent portrayal of older people as vulnerable during the pandemic is, at the very least, an abridged version of the truth.1 Older chronological age is indeed a risk factor for COVID infection, disease severity, and mortality. However, older people are not just a homogenous group of defenceless people in need of protection.
Prurigo pigmentosa and new‐onset type 1 diabetes in a black female patient testing positive for SARS‐CoV‐2 IgG
Authors: K.M. Derrick, D.R. Mazori, E.R. Heilman, S.A. Glick
Journal: British Journal of Dermatology (2020)

Prurigo pigmentosa and new-onset type 1 diabetes in a black female patient
Pre- and Post-Operative Stroke Volume Impact After Surgical Aortic Valve Replacement for Severe Aortic Stenosis
Authors: Ezequiel Guzzetti, Anthony Poulin, Mohamed-Salah Annabi, Dimitri Kalavrouziotis, François Dagenais, Philippe Pibarot, Marie-Annick Clavel
Journal: Journal of the American College of Cardiology (2020)
Effect of Timing of and Adherence to Social Distancing Measures on COVID-19 Burden in the United States
Authors: Oguzhan Alagoz, Ajay K. Sethi, Brian W. Patterson, Matthew Churpek, Nasia Safdar
Journal: Annals of Internal Medicine (2020)

Across the United States, various social distancing measures were implemented to control the spread of coronavirus disease 2019 (COVID-19). However, the effectiveness of such measures for specific regions with varying population demographic characteristics and different levels of adherence to social distancing is uncertain.
TCT CONNECT-461 Predictors of Reversibility of Left Bundle Branch Block After TAVI Implantation
Authors: Carlos Arellano Serrano, Sara Lozano Jiménez, Arturo Garcia-Touchard, Jose Antonio Fernandez-Diaz, Maria Del Trigo, Victor Castro, Jorge Toquero, Marta Cobo Marcos, Cristina Mitroi, Ignacio Fernández Lozano, Javier Goicolea, Javier Segovia-Cubero, Juan Francisco Oteo Dominguez
Journal: Journal of the American College of Cardiology (2020)