tbuckley/cabot-search huggingface dataset:
Abstract 21133: Platelet Vesicle-Engineered Stem Cells for Enhanced Delivery to Heart Injury: Rodent and Porcine Studies
Authors: Junnan Tang, Jinying Zhang, Ke Cheng
Journal: Circulation (2017)

Introduction: Stem cell transplantation is currently implemented clinically, but is limited by low retention and insufficient targeting to the injured heart. Platelets have the natural ability to home to injury and play an important in recruiting stem cells to the sites of injury on blood vessels. Hypothesis: We seek to harness the natural injury-homing ability of platelets to enhance the vascular delivery of cardiac stem cells (CSCs) to myocardial infarction (MI) injury. Methods: We fuse platelet nanovesicles (PNVs) onto the surface of CSCs to form engineered PNV-CSCs. This strategy is nontoxic to CSCs. The ability of PNV-CSCs for binding onto the injured vessel is tested ex vivo . Rat and pig models of ischemia/reperfusion are established to test PNV-CSCs. Results: PNV-CSCs possessed surface markers of platelets, which are associated with platelet adhesion to injury sites. PNV-CSCs can selectively bind collagen-coated surface and denuded rat aorta ex vivo . In rats and pigs with ischemia/reperfusion injury, PNV decoration increases CSC retention in the heart and augments therapeutic benefits. PNV-CSCs treatment robustly boosts cardiac function with the highest left ventricular ejection fraction and best cardiac morphology by promoting angiomyogenesis. Conclusions: The engineered PNV-CSCs possess the natural targeting and repairing ability of their parental cells: platelets and of CSCs. This method offers fast, straightforward, and safe manipulation of stem cells with no genetic alterations of the cells and is generalizable to multiple cell types.
Abstract 179: Evaluation Of Coronary Artery Lesion In 9 Infantile Kawasaki Disease Patients Using 320row Area Detector Ct
Authors: Yoshimitsu Tsuzuki, Masanori Mizuno, Osada Yousuke, Marie Nakano, Kenzo Sakurai, Chikako Masumori, Masaki Arima, Kenzirou Gotou, Kentarou Aso
Journal: Circulation (2015)

Introduction: Recent advances of CT technology have made it possible to obtain clear coronary artery lesion (CAL) image. However, high dose radiation exposure is inevitable. Also it becomes difficult to obtain adequate images when patient’s heart rate is high. Recently 320row area detector CT (ADCT) has been introduced. Using this device, clear coronary artery images are obtainable with low dose radiation exposure. Also it provides adequate image for high heart rate patients. However, there is few report which describe its utility for infantile patients. Aim: To evaluate the feasibility of 320row area detector CT for evaluation of CAL for infantile KD patients. Subjects and methods: Nine infants who have CAL due to KD have been evaluated. Mean age at examination was 30.5+/-22.0 (mean+/-SD) months, weight was 13.6+/-6.5 kg and mean time elapse from diagnosis of KD was 18.7+/-16.0 months. Patients were evaluated using ADCT and findings obtained were compared with either coronary angiography (CAG) or echocardiography. Radiation exposure dose were also explored. Results: All patients have accomplished examination and adequate images were obtained. Mean heart rate at examination was 105+/-27 bpm. Examinations were conducted to two patients with breath holding and others with spontaneous breathing. Compared with CAG and echocardiography, similar results were obtained. Mean effective radiation dose was 6.14+/-2.22 mSv. Conclusion: Infantile KD patient’s CALs were able to be evaluated by ADCT with relatively low dose radiation exposure, without breath holding. ADCT have contributed to reduce the need for repeated invasive CAG.
Thyrotropin aggravates atherosclerosis by promoting macrophage inflammation in plaques
Authors: Chongbo Yang, Ming Lu, Wenbin Chen, Zhao He, Xu Hou, Mei Feng, Hongjia Zhang, Tao Bo, Xiaoming Zhou, Yong Yu, Haiqing Zhang, Meng Zhao, Laicheng Wang, Chunxiao Yu, Ling Gao, Wenjian Jiang, Qunye Zhang, Jiajun Zhao
Journal: The Journal of Experimental Medicine (2019)

Subclinical hypothyroidism is associated with cardiovascular diseases, yet the underlying mechanism remains largely unknown. Herein, in a common population (n = 1,103), TSH level was found to be independently correlated with both carotid plaque prevalence and intima-media thickness. Consistently, TSH receptor ablation in ApoE-/- mice attenuated atherogenesis, accompanied by decreased vascular inflammation and macrophage burden in atherosclerotic plaques. These results were also observed in myeloid-specific Tshr-deficient ApoE-/- mice, which indicated macrophages to be a critical target of the proinflammatory and atherogenic effects of TSH. In vitro experiments further revealed that TSH activated MAPKs (ERK1/2, p38α, and JNK) and IκB/p65 pathways in macrophages and increased inflammatory cytokine production and their recruitment of monocytes. Thus, the present study has elucidated the new mechanisms by which TSH, as an independent risk factor of atherosclerosis, aggravates vascular inflammation and contributes to atherogenesis.
Five Years after Frontline Tyrosine-Kinase Inhibitor (TKI) Treatment Initiation for Chronic Myeloid Leukemia: What Does It Happen in a Real-Life Setting?
Authors: Daniela De Benedittis, Massimo Breccia, Ida Carmosino, Elena Mariggiò, Matteo Molica, Sara Mohamed, Fulvio Massaro, Maria Lucia De Luca, Emilia Scalzulli, Chiara Lisi, Mauro Passucci, Gioia Colafigli, Maria Giovanna Loglisci, Daniela Diverio, Marco Mancini, Robin Foà, Roberto Latagliata
Journal: Blood (2018)
Clinical and Molecular Features of Post-Colonoscopy Colorectal Cancers
Authors: N. Jewel Samadder, Deb Neklason, Angela Snow, Wade Samowitz, Melissa H. Cessna, Kerry Rowe, Iqbal Sandhu, Kenneth Boucher, Lisa Pappas, Ken Robert Smith, Jathine Wong, Karen Curtin, Dawn Provenzale, Randall W. Burt
Journal: Clinical Gastroenterology and Hepatology (2019)
Universities’ data systems can be hacked in under two hours, cybersecurity testing shows
Authors: Ingrid Torjesen
Journal: BMJ (2019)

A test of cyber defences at UK universities has found that hackers are able to get into data systems and gain access to “high value” information, including research data, in just two hours.

Simulated attacks carried out by Jisc, the agency providing internet and other shared digital services to the UK’s universities and research centres, gained access to personal data, finance systems, and research networks within two hours in all of the higher education institutions it targeted.

The techniques used by …
Still puzzling about a clear definition of pulmonary arterial hypertension in newborns
Authors: Giuseppe Calcaterra, Vassilios Fanos, Pier Paolo Bassareo
Journal: European Respiratory Journal (2019)

We read with close attention the report recently published in the European Respiratory Journal by Rosenzweig et al . [1]. A more in-depth, refined definition of pulmonary arterial hypertension in newborns is needed <http://ow.ly/DQH530nDaLz>
A video-based, flipped classroom, simulation curriculum for dermatologic surgery: A prospective, multi-institution study
Authors: Kristina J. Liu, Elizabeth Tkachenko, Abigail Waldman, Marko T. Boskovski, Rebecca I. Hartman, Adriane A. Levin, Bichchau M. Nguyen, Emily S. Ruiz, Victoria R. Sharon, Laura Sowerby, Jeffrey Tiger, F. Clarissa Yang, Arash Mostaghimi
Journal: Journal of the American Academy of Dermatology (2019)
Prospective evaluation of a primary care referral pathway for patients with non-alcoholic fatty liver disease
Authors: Ankur Srivastava, Ruth Gailer, Sudeep Tanwar, Paul Trembling, Julie Parkes, Alison Rodger, Deepak Suri, Douglas Thorburn, Karen Sennett, Sarah Morgan, Emmanuel A. Tsochatzis, William Rosenberg
Journal: Journal of Hepatology (2019)

Highlights•Established blood tests can be used in primary care to stratify patients with fatty liver disease.•A 2-step pathway (FIB-4 followed by ELF™ if required) reduced unnecessary referrals by 80%.•This pathway also improved the detection of cases of advanced fibrosis 5-fold and cirrhosis 3-fold.•This pathway can be used in primary care to identify patients who might benefit from referral to liver specialists.•This should reduce unnecessary referrals while at the same time improving the detection of cirrhosis.Graphical abstractAbstractBackground & AimsThe development of non-invasive liver fibrosis tests may enable earlier identification of patients with non-alcoholic fatty liver disease (NAFLD) requiring referral to secondary care. We developed and evaluated a pathway for the management of patients with NAFLD, aimed at improving the detection of cases of advanced fibrosis and cirrhosis, and avoiding unnecessary referrals.MethodsThis was a prospective longitudinal cohort study, with analyses performed before and after introduction of the pathway, and comparisons made to unexposed controls. We used a 2-step algorithm combining the use of Fibrosis-4 score followed by the ELF™ test if required.ResultsIn total, 3,012 patients were analysed. Use of the pathway detected 5 times more cases of advanced fibrosis (Kleiner F3) and cirrhosis (odds ratio [OR]5.18;95%CI2.97–9.04; p <0.0001), while reducing unnecessary referrals from primary care to secondary care by 81% (OR0.193; 95%CI 0.111–0.337; p <0.0001). Although it was used for only 48% of referrals, significant benefits were observed in practices exposed to the pathway compared to those which were not, with unnecessary referrals falling by 77% (OR0.23; 95% CI0.658–0.082; p = 0.006) and a 4-fold improvement in detection of cases of advanced fibrosis and cirrhosis (OR4.32; 95% CI1.52–12.25; p = 0.006). Compared to referrals made before the introduction of the pathway, unnecessary referrals fell from 79/83 referrals (95.2%) to 107/152 (70.4%), representing an 88% reduction in unnecessary referrals when the pathway was followed (OR0.12; 95%CI0.042–0.349; p <0.0001).ConclusionsThe use of non-invasive blood tests for liver fibrosis improves the detection of advanced fibrosis and cirrhosis, while reducing unnecessary referrals in patients with NAFLD. This strategy improves resource use and benefits patients.Lay summaryNon-alcoholic fatty liver disease effects up to 30% of the population but only a minority of cases develop liver disease. Our study has shown that established blood tests can be used in primary care to stratify patients with fatty liver disease, leading to a reduction in unnecessary referrals by 80% and greatly improving the detection of cases of advanced fibrosis and cirrhosis.
A Human Hematopoietic Niche Model Supporting Hematopoietic Stem and Progenitor Cells In Vitro
Authors: Maaike V. J. Braham, Amélie S. P. Li Yim, Jara Garcia Mateos, Monique C. Minnema, Wouter J. A. Dhert, F. Cumhur Öner, Catherine Robin, Jacqueline Alblas
Journal: Advanced Healthcare Materials (2019)

Abstract Niches in the bone marrow regulate hematopoietic stem and progenitor cell (HSPC) fate and behavior through cell–cell interactions and soluble factor secretion. The niche‐HSPC crosstalk is a very complex process not completely elucidated yet. To aid further investigation of this crosstalk, a functional in vitro 3D model that closely represents the main supportive compartments of the bone marrow is developed. Different combinations of human stromal cells and hydrogels are tested for their potential to maintain CD34 + HSPCs. Cell viability, clonogenic hematopoietic potential, and surface marker expression are assessed over time. Optimal HSPC support is obtained in presence of adipogenic and osteogenic cells, together with progenitor derived endothelial cells. When cultured in a bioactive hydrogel, the supportive cells self‐assemble into a hypoxic stromal network, stimulating CD34 + CD38 + cell formation, while maintaining the pool of CD34 + 38 − HSPCs. HSPC clusters colocalize with the stromal networks, in close proximity to sinusoidal clusters of CD31 + endothelial cells. Importantly, the primary in vitro niche model supports HSPCs with no cytokine addition. Overall, the engineered primary 3D bone marrow environment provides an easy and reliable model to further investigate interactions between HSPCs and their endosteal and perivascular niches, in the context of normal hematopoiesis or blood‐related diseases.
Closing the Gender Wage Gap and Achieving Professional Equity in Medicine
Authors: Maryam M. Asgari, Phyllis L. Carr, Carol K. Bates
Journal: JAMA (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Pluripotent stem cell-derived bile canaliculi-forming hepatocytes to study genetic liver diseases involving hepatocyte polarity
Authors: Arend W. Overeem, Karin Klappe, Silvia Parisi, Petra Klöters-Planchy, Lavinija Mataković, Marines du Teil Espina, Christian A. Drouin, Karl Heinz Weiss, Sven C.D. van IJzendoorn
Journal: Journal of Hepatology (2019)
Membrane Cholesterol Efflux Drives Tumor-Associated Macrophage Reprogramming and Tumor Progression
Authors: Pieter Goossens, Juan Rodriguez-Vita, Anders Etzerodt, Marion Masse, Olivia Rastoin, Victoire Gouirand, Thomas Ulas, Olympia Papantonopoulou, Miranda Van Eck, Nathalie Auphan-Anezin, Magali Bebien, Christophe Verthuy, Thien Phong Vu Manh, Martin Turner, Marc Dalod, Joachim L. Schultze, Toby Lawrence
Journal: Cell Metabolism (2019)
Second‐line treatments in children with immune thrombocytopenia: Effect on platelet count and patient‐centered outcomes
Authors: Rachael F. Grace, Kristin A. Shimano, Rukhmi Bhat, Cindy Neunert, James B. Bussel, Robert J. Klaassen, Michele P. Lambert, Jennifer A. Rothman, Vicky R. Breakey, Kerry Hege, Carolyn M. Bennett, Melissa J. Rose, Kristina M. Haley, George R. Buchanan, Amy Geddis, Adonis Lorenzana, Michael Jeng, Yves D. Pastore, Shelley E. Crary, Michelle Neier, Ellis J. Neufeld, Nolan Neu, Peter W. Forbes, Jenny M. Despotovic
Journal: American Journal of Hematology (2019)

Immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with isolated thrombocytopenia and hemorrhagic risk. While many children with ITP can be safely observed, treatments are often needed for various reasons, including to decrease bleeding, or to improve health related quality of life (HRQoL). There are a number of available second-line treatments, including rituximab, thrombopoietin-receptor agonists, oral immunosuppressive agents, and splenectomy, but data comparing treatment outcomes are lacking. ICON1 is a prospective, multi-center, observational study of 120 children starting second-line treatments for ITP designed to compare treatment outcomes including platelet count, bleeding, and HRQoL utilizing the Kids ITP Tool (KIT). While all treatments resulted in increased platelet counts, romiplostim had the most pronounced effect at 6 months (P = .04). Only patients on romiplostim and rituximab had a significant reduction in both skin-related (84% to 48%, P = .01 and 81% to 43%, P = .004) and non-skin-related bleeding symptoms (58% to 14%, P = .0001 and 54% to 17%, P = .0006) after 1 month of treatment. HRQoL significantly improved on all treatments. However, only patients treated with eltrombopag had a median improvement in KIT scores at 1 month that met the minimal important difference (MID). Bleeding, platelet count, and HRQoL improved in each treatment group, but the extent and timing of the effect varied among treatments. These results are hypothesis generating and help to improve our understanding of the effect of each treatment on specific patient outcomes. Combined with future randomized trials, these findings will help clinicians select the optimal second-line treatment for an individual child with ITP.
Nonanimal Models for Acute Toxicity Evaluations: Applying Data-Driven Profiling and Read-Across
Authors: Daniel P. Russo, Judy Strickland, Agnes L. Karmaus, Wenyi Wang, Sunil Shende, Thomas Hartung, Lauren M. Aleksunes, Hao Zhu
Journal: Environmental Health Perspectives (2019)

Background: Low-cost, high-throughput in vitro bioassays have potential as alternatives to animal models for toxicity testing. However, incorporating in vitro bioassays into chemical toxicity evaluations such as read-across requires significant data curation and analysis based on knowledge of relevant toxicity mechanisms, lowering the enthusiasm of using the massive amount of unstructured public data. Objective: We aimed to develop a computational method to automatically extract useful bioassay data from a public repository (i.e., PubChem) and assess its ability to predict animal toxicity using a novel bioprofile-based read-across approach. Methods: A training database containing 7,385 compounds with diverse rat acute oral toxicity data was searched against PubChem to establish in vitro bioprofiles. Using a novel subspace clustering algorithm, bioassay groups that may inform on relevant toxicity mechanisms underlying acute oral toxicity were identified. These bioassays groups were used to predict animal acute oral toxicity using read-across through a cross-validation process. Finally, an external test set of over 600 new compounds was used to validate the resulting model predictivity. Results: Several bioassay clusters showed high predictivity for acute oral toxicity (positive prediction rates range from 62–100%) through cross-validation. After incorporating individual clusters into an ensemble model, chemical toxicants in the external test set were evaluated for putative acute toxicity (positive prediction rate equal to 76%). Additionally, chemical fragment–in vitro–in vivo relationships were identified to illustrate new animal toxicity mechanisms. Conclusions: The in vitro bioassay data-driven profiling strategy developed in this study meets the urgent needs of computational toxicology in the current big data era and can be extended to develop predictive models for other complex toxicity end points. https://doi.org/10.1289/EHP3614
Conditional Survival and Mortality Risks after Allogeneic Hematopoietic Cell Transplantation (alloHCT) in Patients Who Survived Varying Length of Time after Allohct
Authors: F. Lennie Wong, Meisi Xiao, Jennifer Berano Teh, Liezl Atencio, Alicia Gonzales, Amy Hinton, David Iukuridze, Stephen J Forman, Ryotaro Nakamura, Saro H. Armenian
Journal: Blood (2018)
Abstract 263: A Decline in Kidney-derived Protein Klotho Contributes to Aging-related Heart Failure
Authors: Zhongjie Sun, Kai Chen
Journal: Circulation Research (2016)

Background &amp; Objective: The heart function declines in the aged population. At age 80 years, LV contractile function is less than half of what it was at age 20 years. Aging is a recognized risk factor for heart diseases. For instance, the prevalence of heart disease increases with age. The mortality from heart diseases is higher in the aged than in the young population. Klotho is a recently discovered anti-aging gene that is primarily expressed in kidneys. The secreted Klotho is released into blood. The objective of this study is to investigate if a decline serum Klotho levels contributes to aging-related heart failure. Method &amp; Results: The results showed that ejection fraction, stroke volume and cardiac output were decreased in aged mice (24 months) vs. the adult mice (10 months), indicating that aging impairs heart function. The serum level of Klotho was decreased significantly in aged mice. Interestingly, daily injection of recombinant Klotho protein rescued the aging-related decline in heart function. Mutation of Klotho gene ( KL -/- ) resulted in heart failure which was associated with a significant increase in serum phosphate levels (hyperphosphatemia). Low phosphate diet delayed but did not prevent the development of heart failure in male KL -/- mice, suggesting that hyperphosphatemia partially contributes to Klotho deficiency-induced heart failure. Unfortunately, low phosphate diet failed to improve heart failure in female KL -/- mice. Interestingly, administration of estrogen decreased hyperphosphatemia and delayed the development of heart failure in KL -/- although it did not prevent Klotho deficiency-induced heart failure. Therefore, Conclusion: Klotho deficiency contributes to aging-related heart failure. Hyperphosphatemia accelerated the development of Klotho deficiency-induced heart failure. Administration of recombinant Klotho protein is an effective therapeutic strategy for heart aging. (supported by NIH R01 HL118558, AG049780, DK093403).
Abstract 20126: Associations of Known Complications of Transcatheter Implantation of Balloon-expandable Edwards-Sapien XT Aortic Valves With Peri-procedural Mortality - An Insight From the 2014-2015 Reports From the FDA MAUDE Database
Authors: Pragya Ranjan, Saurav Chatterjee, Deepansh Gupta, Partha Sardar, Jay Giri, Jasjit Bhinder, Debabrata Mukherjee, Ashish Correa, Dharam J Kumbhani
Journal: Circulation (2015)

Background: Transcatheter implantation of aortic valves (TAVR) has revolutionized the management of severe aortic stenosis. However, specific complications have been noted with the valves. Details of such were sought from the US Food and Drug Administration (FDA) maintained MAUDE database which houses medical device reports submitted to the FDA by mandatory reporters (manufacturers, importers and device user facilities) and voluntary reporters such as health care professionals, patients and consumers. Methods: A detailed search was conducted in PubMed, Cochrane Library, EMBASE and CINAHL to identify adverse events reported with the implantation of the Edwards-Sapien XT valves. Then the FDA MAUDE database was queried from June 2014 to May 2015 to identify prevalence of the same complications as reported to the FDA. Individual patient reports were identified and relevant data on such complications were abstracted. Finally, mortality outcomes from such patients were abstracted, and associations of these for each individual reported complication were identified in a multivariate logistic regression model. Results: FDA MAUDE revealed 730 unique reports of complications with the first- generation Edwards-Sapien valves. A total of 106 patients died peri-procedurally. The complications most strongly associated with peri-procedural mortality were development of peri-procedural vascular complications, need for permanent pacemaker, renal failure, atrial fibrillation, stroke, need for surgical valve implantation and need for valve in valve procedure. (Table 1) Conclusions: Specific complications with the new Edwards XT valves were strongly associated with mortality, and focused strategies to mitigate these complications may improve outcomes with future generations of this exciting new technology.
Expanding therapeutic utility of carfilzomib for breast cancer therapy by novel albumin-coated nanocrystal formulation
Authors: Ji Eun Park, Joonyoung Park, Yearin Jun, Yunseok Oh, Gongmi Ryoo, Yoo-Seong Jeong, Hytham H. Gadalla, Jee Sun Min, Jung Hwan Jo, Myung Geun Song, Keon Wook Kang, Soo Kyung Bae, Yoon Yeo, Wooin Lee
Journal: Journal of Controlled Release (2019)