tbuckley/cabot-search huggingface dataset:
AB1118 THE ROLE OF ANGIOPOETHIN-LIKE PROTEIN 4 TYPE IN PROGRESSION OF INFLAMMATORY CHANGES IN RHEUMATOID ARTHRITIS
Authors: Vladislav Aleksandrov, Ludmila Shilova, Irina Alekhina, Ninel Aleksandrova, Natalia Nikitina, Andrey Aleksandrov
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Angiopoietin-like protein 4 (ANGPTL4) is actively involved in the processes associated with inflammation, angiogenesis and lipid metabolism in rheumatoid arthritis (RA). <h3>Objectives</h3> To study of the effect of ANGPTL4 on the features of the inflammatory process in RA. <h3>Methods</h3> The study included 36 RA patients (aged from 33 to 64 years old). A control group (12 people) comprised healthy individuals aged 28 to 52 years old, 28 patients with osteoarthritis (OA) aged 48 to 70 years and 14 people with ankylosing spondylitis (AS) aged 39 to 62 years. Levels of ANGPTL4 in serum were determined by the enzyme immunoassay using the commercial test systems «Human Angiopoietin-like Protein 4 ELISA» from «Bio Vendor» (Czech Republic). Serum C-reactive protein (CRP) levels, erythrocyte sedimentation rates (ESR), rheumatoid factor (RF) titers, and anti-cyclic citrullinated peptide antibody (anti-CCP) were also measured in patients with RA. <h3>Results</h3> The following results were obtained: the level of ANGPTL4 was significantly higher in patients with RA than in patients with OA, AS, and healthy individuals (p = 0.04, p = 0.021, p = 0.038, respectively). A strong positive correlation was found between the level of ANGPTL4 and the activity of RA according to DAS28 (r = 0.71, p = 0.002). There is no reliable association between ANGPTL4 and anti-CCP (p&gt; 0.05). The ANGPTL4 level in the serum was correlated with levels of ESR (r = 0.42, p = 0.019), CRP (r = 0.49, p = 0.007) and the Sharp score of radiologic change (r = 0.39, p = 0.045) in RA. Hypervascularization rates were significantly correlated with ANGPTL4 in patients with RA (r = 0.38, p = 0.002) according to Doppler data. ANGPTL4 can activate proliferation processes in the synovial membrane by binding to integrin-αvβ3. Besides, the concentration of mast cells is increased in the synovium of affected joints. Mast cells significantly influence angiogenesis through the production of proangiogenic cytokines, including ANGPTL4. <h3>Conclusion</h3> Changes of the level of ANGPTL4 in the serum of patients with RA may be a potential biomarker of disease activity, severity of neovascularization, inflammation and development of bone erosion. <h3>Disclosure of Interests</h3> None declared
Abstract 20016: Extra Corporeal Life Support: Bleeding Complications and Out-hospital Survival
Authors: Andre Vincentelli, Natacha Rousse, Francis Juthier, Agnes Mugnier, Emmanuel Robin, Mouhamed Moussa, Carlo Banfi, Antoine Rauch, Eric Van Belle, Sophie Susen
Journal: Circulation (2017)

Introduction: Out-hospital survival after ECLS support is rarely reported. We retrospectively reviewed our institutional data over a15-year experience of ECLS. We analyzed patient survival and risk...
Estimated Contributions of Emissions Controls, Meteorological Factors, Population Growth, and Changes in Baseline Mortality to Reductions in Ambient PM2.5 and PM2.5-Related Mortality in China, 2013–2017
Authors: Dian Ding, Jia Xing, Shuxiao Wang, Kaiyun Liu, Jiming Hao
Journal: Environmental Health Perspectives (2019)

Background: In 2013, China released the Air Pollution Prevention and Control Action Plan (Action Plan), which set the roadmap for national air pollution control actions for the period of 2013 to 2017. A decrease in the fine particulate matter with aerodynamic diameter ≤2.5μm (PM2.5) concentration may lead to a substantial benefit for human health. Objective: We aimed to quantify the relative contributions four factors: emission reductions, changed meteorology, population growth, and a change in baseline mortality rates to the reduced PM2.5-related mortality (PM2.5-mortality) during the 2013–2017 period and evaluate the importance of emission controls for human health protection in China. Methods: The integrated exposure–response function was adopted to estimate the chronic health effects of PM2.5. The annual PM2.5 concentrations were estimated from chemical transport model simulations combined with surface observations for 2013 and 2017. Relative contributions to PM2.5-mortality from emission reductions and the three factors were individually quantified through scenario analysis. Results: The estimated total PM2.5-mortality in China was 1.389 million [95% confidence interval (CI): 1.005 million, 1.631 million] in 2013 but was substantially reduced to 1.102 million (95% CI: 0.755 million, 1.337 million) in 2017. Emission controls contributed 88.7% to this reduction in PM2.5-mortality, while changed meteorology, the change in baseline mortality rates, and population growth during 2013–2017 contributed 9.6, 3.8, and −2.2%, respectively. Conclusions: The implementation of the Action Plan has significantly reduced the PM2.5 concentration in regions of China where population density is high, dominating the decline in PM2.5-mortality during 2013–2017. However, the health burden of PM2.5 pollution in China is still extremely high compared with that in other developed countries. An aggressive air pollution control strategy should be implemented in densely populated areas to further reduce the health burden. https://doi.org/10.1289/EHP4157
S870 SINTILIMAB FOR RELAPSED/REFRACTORY (R/R) EXTRANODAL NK/T CELL LYMPHOMA (ENKTL): A MULTICENTER, SINGLE‐ARM, PHASE 2 TRIAL (ORIENT‐4)
Authors: R. Tao, L. Fan, Y. Song, Y. Hu, W. Zhang, Y. Wang, L. Xu, H. Zhou, J. Li
Journal: HemaSphere (2019)

Background: ENKTL account for more than 20% of the peripheral T‐cell lymphoma in Asia. Patients with r/r ENKTL have a poor prognosis after failing an L‐asparaginase based regimen, and the median overall survival is less than 6 months. The overexpression of PD‐L1 induced by EBV infection is a potential mechanism for ENKTL to avert immune surveillance, and recent studies of PD‐1 antibodies in pts with r/r ENKTL have demonstrated potential efficacy. Sintilimab, a fully human anti‐PD‐1 monoclonal antibody, has a safety profile consistent with other approved PD‐1 antibodies and was approved for r/r classical Hodgkin lymphoma in China in 2018. Aims: This multicenter, single‐arm, phase 2 study aims to validate the efficacy and safety of sintilimab monotherapy in patients with r/r ENKTL in China. Methods: Patients with pathologically confirmed r/r ENKTL were enrolled. Sintilimab was given 200 mg IV Q3W, until PD, death, unacceptable toxicity, or withdrawal from the study. Treatment beyond PD is allowed. Tumor response evaluation was performed by both PET‐CT and CT/MRI with contrast. The primary endpoint was objective response rate based on LUGANO 2014 criteria. Data cut‐off date for this analysis was Feb 2, 2019. Results: From Aug 31, 2017 to Feb 7, 2018, a total of 28 patients were enrolled: 60.7% male and the median age was 37 (range: 19∼65) yr. Sixty‐eight percent of patients were stage IV and 89.3% were ECOG PS ≥ 1. All patients had failed an L‐asparaginase based regimen, the median lines of previous therapy were 3 (range: 1∼13), 78.6% patients received prior radiotherapy and 7.1% had failed HSCT. Median duration of therapy was 14.04 (range: 1.4∼17.3) months and 19 patients are still receiving sintilimab. Sixty‐eight percent (19/28, 95%CI: 47.6%∼84.1%) of patients achieved response (CR+PR), including 4 pts who experienced PD prior to having a response. DCR was 85.7%, including 5 pts who experienced PD before SD or response. The 1‐year OS rate was 82.1% and the median OS has not been reached. Most TRAEs were G1∼2 (67.9%) and no patients discontinued treatment due to AEs. The most common TRAE was decreased lymphocyte count (46.4%) and 84.6% were grade 1∼2. SAEs occurred in 21.4% of patients and none were related to sintilimab. No patients died from AEs. Summary/Conclusion: Sintilimab is effective and well tolerated in r/r ENKTL and could be a promising treatment option for these patients. Early disease progression observed by PET scan in this study could be pseudo‐progression as it did not correlate with poor outcome, which warrants further investigation. NCT03228836
Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression
Authors: Zhuanghui Feng, Yuda Wei, Yongxian Zhang, Yan Qiu, Xiaojian Liu, Li Su, Ningning Liang, Huiyong Yin, Qiurong Ding
Journal: Theranostics (2019)

Identification of proper agents to increase or activate UCP1+ cells in adipose tissues remains a potent therapeutic strategy to combat obesity. Screening systems for UCP1 activators have been previously established and allow for unbiased discovery of effective compound(s). Methods: A previously established Ucp1-2A-GFP reporter system was applied to a chemical library containing 33 phosphatase inhibitors. Compounds that can significantly activate UCP1 expression were further tested in vivo in mouse adipose tissues. Possible underlying mechanism was explored via RNA profiling, CMAP analysis, CRISPR targeting as well as inhibitor treatments. Results: We identified BML-260, a known potent inhibitor of the dual-specific phosphatase JSP-1, that significantly increased UCP1 expression in both brown and white adipocytes. BML-260 treatment also activated oxidative phosphorylation genes, increased mitochondrial activity as well as heat generation in vitro and in vivo. Mechanistic studies revealed that effect of BML-260 on adipocytes was partly through activated CREB, STAT3 and PPAR signaling pathways, and was unexpectedly JSP-1 independent. Conclusion: The rhodanine derivate BML-260 was previously identified to be a JSP-1 inhibitor, and thus was proposed to treat inflammatory and proliferative disorders associated with dysfunctional JNK signaling. This work provides evidences that BML-260 can also exert a JSP-1-independent effect in activating UCP1 and thermogenesis in adipocytes, and be potentially applied to treat obesity.
Abstract 12966: Triple Treatment With Alirocumab and Evinacumab on Top of Atorvastatin Regresses Lesion Size and Improves Plaque Phenotype in APOE*3Leiden.CETP Mice
Authors: Marianne G Pouwer, Elsbet J Pieterman, Worms Nicole, Nanda Keijzer, J W Jukema, Viktoria Gusarova, Jesper Gromada, Hans M Princen
Journal: Circulation (2018)

Introduction: Regression of atherosclerosis beyond the level of lesion size at the start of treatment is modest with the current standard therapy and therefore it may be beneficial to investigate n...
The evolution of CHROMOMETHYLASES and gene body DNA methylation in plants
Authors: Adam J. Bewick, Chad E. Niederhuth, Lexiang Ji, Nicholas A. Rohr, Patrick T. Griffin, Jim Leebens-Mack, Robert J. Schmitz
Journal: Genome biology (2017)

The evolution of gene body methylation (gbM), its origins, and its functional consequences are poorly understood. By pairing the largest collection of transcriptomes (>1000) and methylomes (77) across Viridiplantae, we provide novel insights into the evolution of gbM and its relationship to CHROMOMETHYLASE (CMT) proteins.CMTs are evolutionary conserved DNA methyltransferases in Viridiplantae. Duplication events gave rise to what are now referred to as CMT1, 2 and 3. Independent losses of CMT1, 2, and 3 in eudicots, CMT2 and ZMET in monocots and monocots/commelinids, variation in copy number, and non-neutral evolution suggests overlapping or fluid functional evolution of this gene family. DNA methylation within genes is widespread and is found in all major taxonomic groups of Viridiplantae investigated. Genes enriched with methylated CGs (mCG) were also identified in species sister to angiosperms. The proportion of genes and DNA methylation patterns associated with gbM are restricted to angiosperms with a functional CMT3 or ortholog. However, mCG-enriched genes in the gymnosperm Pinus taeda shared some similarities with gbM genes in Amborella trichopoda. Additionally, gymnosperms and ferns share a CMT homolog closely related to CMT2 and 3. Hence, the dependency of gbM on a CMT most likely extends to all angiosperms and possibly gymnosperms and ferns.The resulting gene family phylogeny of CMT transcripts from the most diverse sampling of plants to date redefines our understanding of CMT evolution and its evolutionary consequences on DNA methylation. Future, functional tests of homologous and paralogous CMTs will uncover novel roles and consequences to the epigenome.
Bayesian Analysis of Evolutionary Divergence with Genomic Data under Diverse Demographic Models
Authors: Yujin Chung, Jody Hey
Journal: Molecular Biology and Evolution (2017)

We present a new Bayesian method for estimating demographic and phylogenetic history using population genomic data. Several key innovations are introduced that allow the study of diverse models within an Isolation-with-Migration framework. The new method implements a 2-step analysis, with an initial Markov chain Monte Carlo (MCMC) phase that samples simple coalescent trees, followed by the calculation of the joint posterior density for the parameters of a demographic model. In step 1, the MCMC sampling phase, the method uses a reduced state space, consisting of coalescent trees without migration paths, and a simple importance sampling distribution without the demography of interest. Once obtained, a single sample of trees can be used in step 2 to calculate the joint posterior density for model parameters under multiple diverse demographic models, without having to repeat MCMC runs. Because migration paths are not included in the state space of the MCMC phase, but rather are handled by analytic integration in step 2 of the analysis, the method is scalable to a large number of loci with excellent MCMC mixing properties. With an implementation of the new method in the computer program MIST, we demonstrate the method’s accuracy, scalability, and other advantages using simulated data and DNA sequences of two common chimpanzee subspecies: Pan troglodytes (P. t.) troglodytes and P. t. verus.
PB2420 EFFICIENCY OF DUAL ANTIPLATELET THERAPY AFTER PERCUTANEOUS CORONARY INTERVENTION
Authors: O. Matvienko, O. Smirnova, I. Gelzer, L. Tarkovskaya, O. Golovina, L. Papayan
Journal: HemaSphere (2019)

Background: Percutaneous coronary intervention (PCI) is the treatment metod of ischemic heart disease. Platelet hyperactivity is associated with stent thrombosis so this patients need antiplatelet therapy. Such therapy usually includes clopidogrel and aspirin. It might be useful to assess total procoagulant activity using a global coagulation test in patients on dual antiplatelet therapy (DAPT) Aims: To estimate the plasma coagulation potential level in patients with a different response to antiplatelet therapy. Methods: This research included 46 patients after the successful coronary stent placement. All of them were given 75 mg clopidogrel and 150 mg aspirin per day. The antiplatelet therapy efficiency was assessed with light transmission aggregometry performed with a Hronolog 4‐channel platelet aggregometer using the adenosine diphosphate (ADP, 5 μM) and collagen (2 μg/ml) as agonists. Maximal platelet aggregation (MA, %) was determined. Thrombin generation was measured in platelet‐rich plasma by Calibrated Automated Thrombinogram Assay method (CAT) using reagent containing recombinant tissue factor (Trombinoscope BV, The Netherlands) as a trigger. Following parameters were derived: endogenous thrombin potential (ETP, nM‐min), peak thrombin (Peak, nM), and rate of thrombin generation (R, nM/min). STATISTICA 6.0 package was used in data analysis. Results are presented as median with 95% confidence intervals, p &lt; 0.05 was considered statistically significant. Results: The results of aggregometry are presented in table. 30 (65%) patients (group №1) had a significant decrease in aggregation response only to collagen compared with controls, the response to ADP was not considerably lower. 16 (35%) patients (group №2) had a significant decrease in aggregation response to both collagen and ADP. The results of platelet aggregation suggest that the patients in the group №2 were more sensitive to antiplatelet therapy. There was a significant decrease in ETP, Peak and R in the group №2 compared with group №1 (ETP:Мe‐1636,5, CI: 1168,6‐1919,9 vs. Me‐1785,0, CI: 1415,7‐2576,2, p &lt; 0,05; Peak: Me‐112,8, CI: 72,3‐138,9 vs. Me‐134,7, CI: 80,6‐180,2, p &lt; 0,001; R: Me‐9,2, CI: 4,1‐13,3 vs. Me‐ 12,4, CI: 4,5‐21,2, p &lt; 0,01). Summary/Conclusion: Patients with a better sensitivity to DAPT had lower plasma coagulation potential. It might be associated with the lower risk of stent thrombosis in patients with good response to DAPT.. Individual assessment of plasma coagulation potential using thrombin generation test could be useful for prediction of the thrombosis risk in the stent in patients after PCI. More research is clearly needed. image
Stereochemical Strategy Advances Microbially Antiadhesive Cotton Textile in Safeguarding Skin Flora
Authors: Jiangqi Xu, Hongjuan Zhao, Zixu Xie, Scott Ruppel, Xiaqing Zhou, Shuang Chen, Jun F. Liang, Xing Wang
Journal: Advanced Healthcare Materials (2019)

Abstract Microbial contamination on cotton textiles (CT) negatively affects people's health as well as the textile itself during use and storage. Using antimicrobial CT in a body‐safe manner is currently still a challenge because it is difficult to balance killing microbes and protecting skin flora. Herein, a borneol‐decorated CT (BDCT) through coupling of borneol 4‐formylbenzoate molecules onto the amino‐modified CT is reported. This BDCT shows strong and broad‐spectrum microbially antiadhesive activities against gram‐positive bacteria ( Staphylococcus aureus and S. epidermidis ), gram‐negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ), and fungi ( Aspergillus niger , Mucor racemosus , and Candida albicans ). Because of its unique stereochemical microbial antiadhesion mechanism, BDCT is harmless to skin flora. In addition, BDCT exhibits prominent durability of microbially antiadhesive capability by bearing 50 times of accelerated laundering. Therefore, this stereochemical BDCT strategy shows great potential for applications in the new generation of textiles, food packaging, and medical protection.
Abstract 15682: Role of Connexin 43 in Human Bone Marrow Derived Mesenchymal Stem Cell Cardiac Integration and Cardiac Stem Cell Niche Formation
Authors: Cristina Sanina, Claudia O Rodrigues, Michael Bellio, Ivonne H Schulman, Wayne Balkan, Konstantinos E Hatzistergos, Irene M Margitich, Joshua M Hare
Journal: Circulation (2013)

Bone marrow-derived mesenchymal stem cells (MSCs) and cardiac progenitor stem cells (CPCs) have been used successfully as a cell-based approach for cardiac regeneration in animal model and in clini...
Revisions of Gastric Bypass—A Moral Obligation—Reply
Authors: Ingmar Näslund, Stephan Hjorth, Lena M. S. Carlsson
Journal: JAMA Surgery (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Abstract 11989: Cardiac 12-Lipoxygenase-Induced Oxidative Stress Promotes the Development of Diabetic Cardiomyopathy
Authors: Yosuke Kayama, Hirofumi Suzuki, Masaya Sakamoto, Ippei Shimizu, Tomohisa Nagoshi, Tohru Minamino, Michihiro Yoshimura
Journal: Circulation (2014)

Diabetes mellitus affects cardiac structure and function, and it has been suggested that diabetes leads to cardiomyopathy. Arachidonic acid (AA) metabolism was thought to be a potential mediator of cardiac fibrosis and heart failure associated with oxidative stress. 12-lipoxygenase (12-LOX) is a key lipid peroxidizing enzyme of the AA cascade that plays an important role in the development of atherogenesis and neurodegenerative disease. However, the role of 12-LOX in diabetic cardiomyopathy has not been examined. To determine whether 12-LOX is a key molecule in the development of diabetic cardiomyopathy, we created streptozotocin (STZ)-induced diabetic mice (WT-STZ) and compared to control mice. Cardiac expression of 12-LOX pathway was up-regulated after induction of diabetes. Histological analysis revealed that expression of 12-LOX was specifically up-regulated in cardiomyocytes but not vascular cells and fibroblast cells. Cardiac fibrosis was increased after induction of diabetes. We next created STZ-induced diabetic mice using 12-LOX KO mice (KO-STZ) and compared them to WT-STZ. Cardiac dysfunction and fibrosis in WT-STZ were significantly inhibited in KO-STZ. We next examined the relationship between 12-LOX and cardiac oxidative stress in the diabetic heart. Cardiac expression of 4-hydroxy-2-nonenal (4-HNE) and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) were up-regulated in the diabetic heart. This increase was inhibited by disruption of 12-LOX. To further investigate the subcellular mechanism of the increase reactive oxygen species (ROS) in the diabetic heart, intracellular ROS levels in cardiomyocytes were estimated under high glucose condition (HG) and normal glucose condition (LG) by fluorimetry and using MitoTracker® Red in vitro. Cardiomyocytes under HG showed the enhancement of fluorescence intensity of DCF-DA and loss of mitochondrial membrane potential. Treatment with 12-LOX inhibitor (CDC) improved the enhancement of DCF-DA and mitochondrial membrane potential under HG condition. These in vivo and in vitro results suggest that 12-LOX pathway is important in the process of production of ROS and oxidative stress in diabetic heart and promotes the development of diabetic cardiomyopathy.