tbuckley/cabot-search huggingface dataset:
P016 Vascular involvement in rheumatic diseases – the role of imaging and immunological biomarkers
Authors: KM Fischer, H Przepiera-Będzak, I Brzosko, M Sawicki, M Brzosko
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Career situation of first and presenting author</h3> Assistant. <h3>Introduction</h3> Vascular abnormalities are common complications in rheumatic patients. Their background is multifactorial and still is a subject of the debate. <h3>Objectives</h3> This study was designed to evaluate the significance of selected noninvasive imaging indices, immunological and genetic biomarkers in diagnosis of vascular lesions in rheumatic diseases. <h3>Methods</h3> The study group included 288 patients with systemic connective tissue diseases (SCTD). The noninvasive evaluation of vascular lesions was made on the basis of carotid intima-media thickness (cIMT), ankle brachial index (ABI), high resistance index (HRI) and ulnar artery intraluminal diameter (UAID) measurements using HDI 3500 (ATL). We analyzed more than 100 variables: autoantibodies, inflammatory and angiogenic markers, genetic polymorphisms and classical risk factors for atherosclerosis. Statistical analysis was performed with STATA 11 including: chi<sup>2</sup>Yates, chi<sup>2</sup>Pearson and rank Spearman correlations tests, logistic regression analysis and multivariate stepwise analysis. <h3>Results</h3> Macroangiopathy was influenced by selected autoantibodies including antiphospholipid (OR=4.4; 95% CI:1.1–20.7) and anti-endothelial cell (OR=6.6; 95% CI:1.6–28.3) as well as inflammatory biomarkers (OR=3.6; 95% CI:1.1–11.8). The analysis of genetic polymorphisms showed especially an important impact of <i>VEGF</i> 2578 AA genotype on atherosclerosis development (OR=4.8; 95% CI:1.1–21.1). Angiogenic biomarkers were strongly associated with prothrombotic risk (OR=22.8; 95% CI:2.3–230.6). The analysis of relations between imaging indices and vascular manifestations revealed significant association of cIMT with cardiovascular (OR=52.9; 95% CI:7.0–1012.7) and cerebrovascular disease (OR=4.0; 95% CI:1.0–15.3). There was significant reverse correlation between ABI and peripheral vascular disease (R=−0.33; p=0.001). HRI values significantly correlated with thromboembolic disorders (R=−0.29; p=0.03). Finaly, UAID was notably related to microangiopathic complications (p&lt;0.05). <h3>Conclusions</h3> The protocol for vascular lesions diagnosis in SCTD should be based on the combination of imaging and laboratory biomarkers. Immunological and inflammatory factors are crucial in diagnostics of vascular involvement in rheumatic diseases. IMT and ABI showed a high prognostic value and can be used for the general cardiovascular risk stratification. <h3>Disclosure of Interest</h3> None declared.
A tangled tale of molecular subtypes in pancreatic cancer
Authors: Shiv Singh, Marie Christin Hasselluhn, Albrecht Neesse
Journal: Gut (2019)

Pancreatic ductal adenocarcinoma (PDAC) constitutes the most common malignancy of the pancreas. With a 5-year survival rate of below 10% and a median survival of less than 12 months following diagnosis, PDAC remains a major biomedical challenge. Due to the lack of early symptoms patients are usually diagnosed with locally advanced or metastatic disease where surgical removal of the tumour is no longer feasible. Despite some recent developments in medical oncology, response to chemotherapeutic regimens such as nab-paclitaxel and FOLFIRINOX is limited and accompanied by side effects such as nausea, neutropenia, neuropathy and infectious complications.1 

Histologically, the majority of PDAC cases are characterised by tubular adenocarcinoma of the ductal glands embedded in a pronounced tumour microenvironment (TME) composed of acellular components such as collagen, hyaluronic acid, fibronectin and abundant matricellular proteins. Moreover, inflammatory cells such as tumour-associated macrophages, tumour-associated neutrophils, regulatory T cells, cancer-associated fibroblasts and neural cells accumulate around neoplastic cells and actively contribute to TME formation and tumour-stroma crosstalk.2 Notably, histopathological features of PDAC have not yet been used to select therapeutic strategies. However, increasing evidence from other tumour entities such as breast, melanoma, lung or colon cancer suggest that molecular characteristics can vastly differ in microscopically indistinguishable cancers. With the advent of high-throughput sequencing technologies such as whole-genome profiling and transcriptome profiling, molecular taxonomy of cancers has become …
Serial assessment of spot urine sodium predicts effectiveness of decongestion and outcome in patients with acute heart failure
Authors: Jan Biegus, Robert Zymliński, Mateusz Sokolski, John Todd, Gad Cotter, Marco Metra, Ewa A. Jankowska, Waldemar Banasiak, Piotr Ponikowski
Journal: European Journal of Heart Failure (2019)
Abstract 16841: Neutrophil-Mediated Docosahexaenoic Acid Oxidation Generates the Substrate for Integrin αMβ2 and αDβ2-Dependent Macrophage Migration
Authors: Valentin P Yakubenko, Kui Cui, Christopher Ardell, Moammir Aziz, Eugene A Podrez, Tatiana Byzova
Journal: Circulation (2017)

A protective role of DHA during cardiovascular diseases and inflammation has been recently questioned based on new data and clinical trials. The reported results demonstrate heterogeneous responses...
Prevalence of antimicrobial resistance genes in Bacteroides spp. and Prevotella spp. Dutch clinical isolates
Authors: A.C.M. Veloo, W.H. Baas, F.J. Haan, J. Coco, J.W. Rossen
Journal: Clinical Microbiology and Infection (2019)

The prevalence of resistance genes in two important anaerobic genera, Bacteroides and Prevotella, was assessed by applying PCR specifically directed to genes of interest.A total of 101 Bacteroides spp. and 99 Prevotella spp. human clinical isolates were identified using MALDI-TOF MS. The presence of the resistance genes cfxA, cepA, cfiA, tetQ, ermF and nim, was assessed. Prevalence of resistance genes was compared with the phenotypic resistance against amoxicillin, clindamycin, meropenem and metronidazole.Even though the majority of the Bacteroides isolates (95.0%) showed resistance towards amoxicillin, only 52/101 of the isolates harboured one of the resistance genes, accounting for this resistance. Within the genus Prevotella the presence of cfxA (50/99) almost perfectly matched the amoxicillin resistance (48/99). No difference in prevalence of the ermF gene (16/101 and 9/99) and clindamycin resistance (16/101 and 10/99) was observed within Bacteroides and Prevotella, respectively. Two isolates of Prevotella were resistant to metronidazole. One harboured the nim gene. One metronidazole-susceptible isolate of Bacteroides harboured a nim gene. Within the Bacteroides and Prevotella genera, 6/101 strains and 5/99 isolates harboured three different resistance genes, respectively, among them tetQ. TetQ is often located on a conjugative transposon, increasing the chance of horizontal gene transfer between isolates.An unknown mechanism in Bacteroides non-fragilis isolates causes resistance to β-lactam antibiotics. The fact that the prevalence of the tetQ gene among Prevotella is increasing and the existence of isolates harbouring three resistance genes are worrisome developments.
Monosomy 7 As the Initial Hit Followed By Sequential Acquisition of SETBP1 and ASXL1 Driver Mutations in Childhood Myelodysplastic Syndromes
Authors: Victor Pastor Loyola, Pritam Kumar Panda, Sushree Sangita Sahoo, Enikoe Amina Szvetnik, Emilia J. Kozyra, Rebecca K Voss, Dirk Lebrecht, Julius Wehrle, Miriam Erlacher, Jan Stary, Christian Flotho, Gudrun Göhring, Brigitte Schlegelberger, Brigitte Strahm, Charlotte Niemeyer, Marcin W. Wlodarski
Journal: Blood (2018)
Sex Differences in Advanced Heart Failure Therapies
Authors: Eileen M. Hsich
Journal: Circulation (2019)

This in-depth review of sex differences in advanced heart failure therapy summarizes the existing literature on implantable cardioverter defibrillators, biventricular pacemakers, mechanical circulatory support, and transplantation with a focus on utilization, efficacy/clinical effectiveness, adverse events, and controversies. One will learn about the controversies regarding efficacy/clinical effectiveness of implantable cardioverter defibrillators and understand why these devices should be implanted in women even if there are sex differences in appropriate shocks. Individuals will learn about the sex differences with biventricular pacemakers with respect to ventricular remodeling and reduction in heart failure hospitalizations/mortality, as well as, possible mechanisms. We will demonstrate sex differences in heart transplantation and waitlist survival. Despite similar survival for women and men with left ventricular assist devices, there are sex differences in adverse events. These devices do successfully bridge women and men to transplant, yet women are less likely than men to have a left ventricular assist at time of listing and time of transplantation. Finally, one will learn about the concerns regarding poor outcome for men who receive female donor hearts and discover this may not be due to sex, but rather size. More research is needed to better understand sex differences and further improve advanced heart failure therapy for both women and men.
GW28-e1215 The characteristics of patients with idiopathic ventricular fibrillation and evaluation of the efficacy with radiofrequency ablation versus ICD: a meta-analysis of individual participant data
Authors: Wenjia Guo, Ping Zhang, Jing Yang, Kun Li, Yajun Xue, Tingting Lv
Journal: Journal of the American College of Cardiology (2017)
Comparison of Biomarker Assays for <i>EGFR</i>: Implications for Precision Medicine in Patients with Glioblastoma
Authors: Andrew B. Lassman, Lisa Roberts-Rapp, Irina Sokolova, Minghao Song, Ekaterina Pestova, Rupinder Kular, Carolyn Mullen, Zheng Zha, Xin Lu, Erica Gomez, Anahita Bhathena, David Maag, Priya Kumthekar, Hui K. Gan, Andrew M. Scott, Maria Guseva, Kyle D. Holen, Peter J. Ansell, Martin J. van den Bent
Journal: Clinical Cancer Research (2019)

Patients with glioblastoma (GBM) have a poor prognosis and are in desperate need of better therapies. As therapeutic decisions are increasingly guided by biomarkers, and EGFR abnormalities are common in GBM, thus representing a potential therapeutic target, we systematically evaluated methods of assessing EGFR amplification by multiple assays. Specifically, we evaluated correlation among fluorescence in situ hybridization (FISH), a standard assay for detecting EGFR amplification, with other methods.Experimental Design: Formalin-fixed, paraffin-embedded tumor samples were used for all assays. EGFR amplification was detected using FISH (N = 206) and whole-exome sequencing (WES, N = 74). EGFR mRNA expression was measured using reverse transcription-polymerase chain reaction (RT-PCR, N = 206) and transcriptome profiling (RNAseq, N = 64). EGFR protein expression was determined by immunohistochemistry (IHC, N = 34). Significant correlations among various methods were determined using Cohen's kappa (κ = 0.61-0.80 defines substantial agreement) or R2 statistics.EGFR mRNA expression levels by RNA sequencing (RNAseq) and RT-PCR were highly correlated with EGFR amplification assessed by FISH (κ = 0.702). High concordance was also observed when comparing FISH to WES (κ = 0.739). RNA expression was superior to protein expression in delineating EGFR amplification.Methods for assessing EGFR mRNA expression (RT-PCR, RNAseq) and copy number (WES), but not protein expression (IHC), can be used as surrogates for EGFR amplification (FISH) in GBM. Collectively, our results provide enhanced understanding of available screening options for patients, which may help guide EGFR-targeted therapeutic approaches.
Lymphoproliferative disease in patients with Wiskott-Aldrich syndrome: Analysis of the French Registry of Primary Immunodeficiencies
Authors: Morgane Cheminant, Nizar Mahlaoui, Céline Desconclois, Danielle Canioni, Loïc Ysebaert, Loïc Dupré, Zilton Vasconcelos, Marion Malphettes, Despina Moshous, Bénédicte Neven, Pierre-Simon Rohrlich, Marc Bernard, Yves Bertrand, Alain Fischer, Felipe Suarez
Journal: Journal of Allergy and Clinical Immunology (2019)
Mitotic Arrest: A possible mechanism for the development of aneuploidy in cancer
Authors: Vanessa Yu, Jennifer Yeh, Louis Dubeau
Journal: Cancer Research (2008)

4228  Aneuploidy, defined as a chromosome number that is not a multiple of the haploid number, is a hallmark of cancer. We previously showed that epithelial cell strains derived from benign ovarian cystadenomas and further transfected with SV40 Large T Antigen undergo polyploidy followed by aneuploidy as they approach in vitro crisis, providing us with an in vitro model to study the consequences of aneuploidy on cancer development. We sought to determine whether the ploidy changes that precede crisis in this in vitro system are triggered by a cell cycle arrest.  To investigate whether an arrest takes place at the G2 checkpoint, we compared the expression levels of the Cdk1/cyclin B1 complex, a mediator of entry into mitosis, and those of the Cdk2/cyclin A complex, a regulator of S phase transition, in cells approaching crisis (primarily aneuploid) versus cells at earlier passages (primarily diploid). Formation of a Cdk1/cyclin B1 complex, and Cdk1 activity were increased in cells approaching crisis, while formation of the Cdk2/cyclin A complex, and Cdk2 activity were decreased in the same cells. A decrease in DNA synthetic activity in cells approaching crisis was also confirmed by comparing the percentage of BrdU incorporation at low versus high passage. These results suggest that fewer cells are transitioning through S phase and undergoing DNA replication as they approach crisis. We further investigated the nature of the cell cycle arrest by comparing the levels of p-histone H3, a marker of nuclear envelope breakdown, in low passage cells versus cells approaching crisis. The levels of this protein were elevated in cells approaching crisis compared to cells undergoing logarithmic growth, further supporting an arrest at the mitotic spindle checkpoint at the time of crisis. Treatment with caffeine, a known abrogator of G2 arrest, caused a decrease in the percentage of cells undergoing G2/M transition, suggesting that this chemical might also be an abrogator of mitotic arrest.  These data suggests that crisis in epithelial cells expressing SV40 Large T Antigen is characterized by a cell cycle arrest at the M phase. The near tetraploidy that characterizes cell populations approaching crisis, as well as cell clones that spontaneously recover from crisis to give rise to immortal cell lines is probably the result of cells overcoming the M phase arrest, leading to the initiation of a new cycle before completion of cytoplasmic separation. Given that a large number of human cancers are near polyploid, this could be an important mechanism of aneuploidy development in cancer.
<i>Circulation Research</i> Editors’ Annual Report for 2006
Authors: Eduardo Marbán, Michelle Strotman, Jonathan Schultz
Journal: Circulation Research (2007)

pleased to present this annual report on the progress of the journal over the course of 2006, the seventh year of our editorship.The journal saw continued overall growth in 2006, with increased submissions, rapid manuscript handling, and enviably brief times to publication.
Spotting the old foe—revisiting the case definition for TB
Authors: Rein M G J Houben, Hanif Esmail, Jon C Emery, Louis R Joslyn, C Finn McQuaid, Nicolas A Menzies, Joaquín Sanz, Sourya Shrestha, Richard G White, Chongguang Yang, Frank Cobelens
Journal: The Lancet Respiratory Medicine (2019)
Identifying Efficacious Treatment Elements for Refractory Conditions, Such as Body Dysmorphic Disorder
Authors: Douglas S. Mennin
Journal: JAMA Psychiatry (2019)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Psychiatry HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Psychiatry journal
Prevalence of mental disorders in China: a cross-sectional epidemiological study
Authors: Yueqin Huang, Yu Wang, Hong Wang, Zhaorui Liu, Xin Yu, Jie Yan, Yaqin Yu, Changgui Kou, Xiufeng Xu, Jin Lu, Zhizhong Wang, Shulan He, Yifeng Xu, Yanling He, Tao Li, Wanjun Guo, Hongjun Tian, Guangming Xu, Xiangdong Xu, Yanjuan Ma, Linhong Wang, Limin Wang, Yongping Yan, Bo Wang, Shuiyuan Xiao, Liang Zhou, Lingjiang Li, Liwen Tan, Tingting Zhang, Chao Ma, Qiang Li, Hua Ding, Hongchun Geng, Fujun Jia, Jianfei Shi, Shiliang Wang, Ning Zhang, Xinbai Du, Xiangdong Du, Yue Wu
Journal: The Lancet Psychiatry (2019)
Sudden deafness: hyperbaric oxygen therapy should be discussed
Authors: J Alexander de Ru, Ahmed B Bayoumy
Journal: BMJ (2019)

The rate of spontaneous complete recovery from sudden deafness is only 39%, so additional therapy is warranted.1 Fishman and Cullen do not mention hyperbaric oxygen therapy in their paragraph on how patients with sudden hearing loss should be managed.2 The Cochrane systematic review finds a significant positive effect for this treatment, which is more than …
Update in Tuberculosis 2006
Authors: Wing Wai Yew, Chi Chiu Leung
Journal: American Journal of Respiratory and Critical Care Medicine (2007)

Because little was known concerning the scope of treatment of latent tuberculosis infection (LTBI) in the United States and Canada, identification of the types of clinics that administered such treatment, and patients who received it, would guide resource utilization and improve treatment initiation and completion. Sterling and coworkers, on behalf of the Tuberculosis Epidemiologic Studies Consortium, Centers for Disease Control and Prevention, surveyed 244 clinics, each having initiated LTBI treatment for 10 or more patients in 2002, at 19 U.S. and 2 Canadian sites (1). An estimated 37,857 patients started LTBI treatment in 2002, including 37,145 from the U.S. sites, with 79% at general public health clinics, 6.4%at immigrant/refugee clinics, and 6.1% at correction/detention facilities. Study catchment areas for the 19 U.S. sites represented 8.6% of the U.S. population and 12.7% of all tuberculosis (TB) cases in 2000. On extrapolation to the entire U.S. population, the estimated total number of LTBI treatment starts was approximately 291,000 to 433,000. Assuming a 5% lifetime risk of TB without treatment, and 20 to 60% treatment effectiveness, approximately 4,000 to 11,000 cases of TB were prevented in the United States. Thus, Sterling and coworkers concluded that treatment for LTBI was initiated among a substantial number of persons in theUnited States andCanada, primarily in the public sector, and such treatment could significantly decrease the disease burden in these countries. Targeted screening and treatment of latently infected subjects are central to strategies aimed at eliminating TB. Unfortunately, there appear to be few specific criteria, other than medical factors, in designating groups as high risk for developing TB. Moonan and coworkers conducted location-based screenings in partnership with multiple community-based organizations in communities previously demonstrated by geographic information system to have genotypically clustered Mycobacterium tuberculosis isolates (2). One person with TB was found for every 83 screened, and one person with LTBI for every five screened, far exceeding the expected yield of untargeted screening for a county with a TB incidence of only 5.7 per 100,000. Male subjects were more commonly identified (odds ratio [OR], 4.8). Thus, it appeared that combining genotyping and geographic information systems could potentially help in identifying high-risk status and in determining areas for location-based TB screening. In an editorial accompanying Moonan and colleagues’ article (2), it was pointed out that these data could be used as a tool for garnering the critical support of community-based organizations (3). Both shortand long-term benefits of such partnerships as well as the resulting interventions are important to measure. In addition to following up on the outcome of the intervention, an analysis of the services received by the screened individuals would clarify the relative roles of housing, correctional care
Stat6 is constitutively activated in human follicular lymphoma
Authors: Seema Rawal, Soung-Chul Cha, Hong Qin, Larry Kwak, Sattva Neelapu
Journal: Cancer Research (2008)

5272  Signal transducers and activators of transcription (STAT) are a family of transcription factors that regulate a broad range of cellular processes, such as proliferation, differentiation, and survival. Constitutive activation of STATs, particularly STAT3, STAT5, and STAT6 has been detected at high frequency in various malignancies. Here, we show that STAT6 is constitutively activated and may a play a role in the pathogenesis of human follicular lymphoma, the most common low-grade B-cell non-Hodgkin’s lymphoma that is considered incurable in advanced stages. STAT6 was demonstrated to be constitutively tyrosine phosphorylated in primary human follicular lymphoma samples by immunoblotting and phosphoflow analysis. We show that IL-4 but not IL-13 is expressed by primary follicular lymphoma cells and may act in an autocrine fashion to induce constitutive phosphorylation of STAT6. Inhibition of STAT6 phosphorylation with a STAT6 inhibitor, leflunidomide resulted in dephosphorylation of STAT6 and enhanced the apoptosis of primary follicular lymphoma cells as determined by Annexin V staining. Furthermore, the addition of exogenous IL-4 protected the cells from undergoing apoptosis in the presence of leflunidomide. Constitutive phosphorylation of STAT6 was associated with expression of growth factor independence-1 (Gfi-1) protein in primary follicular lymphoma samples. Gfi-1 is known to control the transcription of cell cycle proteins, such as decreasing levels of cyclin dependent kinase inhibitor p27Kip1. In addition, Gfi-1 has been shown to inhibit cell death by repressing pro-apoptotic factor Bax. Consistent with this, we observed downregulation of Gfi-1 protein following incubation of primary follicular lymphoma tumor cells with leflunidomide as compared with basal levels. Taken together, these results suggest that constitutive activation of STAT6 may play a role in the pathogenesis of human follicular lymphoma by promoting cell growth and by preventing apoptosis. Moreover, these results imply that inhibitors of STAT6 phosphorylation may be therapeutically useful for the treatment of patients with follicular lymphoma.
<i>Circulation Research</i> Editors’ Yearly Report: 2003
Authors: Eduardo Marbán, Roberto Bolli, Gerda Breitwieser, Rudi Busse, Hal Dietz, Masao Endoh, Toren Finkel, Kathy Griendling, David Kass, Charles Lowenstein, Marlene Rabinovitch, Gordon Tomaselli, Kara Hansell Keehan
Journal: Circulation Research (2004)