tbuckley/cabot-search huggingface dataset:
AB0126 ANTI-INFLAMMATORY EFFECTS OF SPLEEN TYROSINE KINASE (SYK) INHIBITOR, PICEATANNOL, ON FIBROBLAST-LIKE SYNOVIOCYTE IN RHEUMATOID ARTHRITIS
Authors: Seon Uk Kim, Hyun Jung Yoo, Shin Eui Kang, Ji Soo Park, Ra Ham Kim, Jin Kyun Park, Eun Young Lee, Yeong Wook Song
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background:</h3> Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation with subsequent cartilage and bone erosion leading to joint destruction. The fibroblast-like synoviocytes (FLS) have a central role in disease pathogenesis and in vitro FLS invasiveness correlates with articular damage in RA patients. Spleen tyrosine kinase (Syk) is an intracellular protein tyrosine kinase in the cells of joint tissues such as bone, cartilage, and synovium, where it is involved in tumor necrosis factor α (TNF-α) receptor signaling. Piceatannol, a naturally occurring hydroxylated analog of resveratrol, was reported to inhibit Syk. <h3>Objectives:</h3> The aim of this study is to evaluate the effects of piceatannol on inflammation and the downstream signaling pathway of Syk in RA FLS. <h3>Methods:</h3> FLS isolated from synovium of rheumatoid arthritis (RA) patients were cultured with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin. FLS were stimulated with lipopolysaccharide (LPS) for 24 hr after 1 hr treatment of piceatannol. The cytokines were screened using human inflammatory and matrix metalloproteinase (MMP) antibody array kit (Abcam) in the culture supernatant. Also, IL-6, IL-8 and CXCL10 levels were measured by ELISA and the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) were determined by western blotting. <h3>Results:</h3> Protein expression level of Syk was suppressed by piceatannol (50 μM treatment). Piceatannol inhibited the production of cytokines/chemokines such as IL-6, CCL-5, CCL-8 and MMP-1 in RA FLS stimulated with LPS. The levels of IL-6, IL-8 and CXCL10 in RA FLS were dose-dependently decreased. The viability and proliferation of the cells were not affected. Piceatannol significantly suppressed COX-2 and iNOS protein expressions. <h3>Conclusion:</h3> Piceatannol had suppressive effects on pro-inflammatory cytokines/chemokines production and had anti-inflammatory effects in RA FLS. <h3>References</h3> [1] Kjelgaard-Petersen CF, et al. Arthritis Rheumatol. 2018Sep;70(9):1419-1428 [2] Mullard A. et al. Nature Reviews Drug Discovery2018, 17:385 <h3>Disclosure of Interests:</h3> None declared
AB0477 THE EFFECT OF CLOSTRIDIUM BUTYRICUM ON INTESTINAL FLORA OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS
Authors: Zhang Mingxing, Xu-Fang Yin, Li Xiao-Feng
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Objectives:</h3> To investigate the effect of Clostridium butyricum on intestinal flora in patients with systemic lupus erythematosus. <h3>Methods:</h3> Forty-four patients with systemic lupus erythematosus who were randomly selected from our hospital were given the oral administration of Clostridium butyricum live capsule 840mg twice a day. The concentration of methane and hydrogen at each time point before and after treatment for 44 patients was detected and compared by hydrogen and methane in lactulose breath test (LBT). <h3>Results:</h3> After 28 days of treatment with Clostridium butyricum capsules, there was no significantly statistical difference in exhaled hydrogen concentration or methane concentration between 0 min, 30 min, 60 min and 90 min before and after treatment. See Table 1. <h3>Conclusion:</h3> Numerous studies have shown that taking probiotics can promote the growth of normal flora and reduce the reproduction of other abnormal flora, which has a certain impact on the structure and quantity of There was no significantly statistical difference in exhaled hydrogen concentration or methane concentration at various points in time between the 44 patients before and after treatment, suggesting that there was no significant change in the structure and quantity of intestinal flora. intestinal flora, which may be resulted from our low dose Clostridium butyricum live capsules or short treatment time, or need to be combined with other probiotics. These hypothesis above need us to further explore, in general, using microbes as a therapeutic target spot to regulate the illness of SLE by interfering with intestinal flora through diet, probiotics or fecal transplantation provides a promising prospect for clinical treatment of SLE. Using Wilcoxon test, P&lt;0.05. <h3>Disclosure of Interests:</h3> None declared
SAT0330 VALUE OF DIAPHRAGMATIC ASSESSMENT IN RELATION TO PULMONARY FUNCTIONS AND DISEASE ACTIVITY IN AXIAL SPONDYLOARTHROPATHIES: PRELIMINARY REPORT
Authors: Seçilay Güneş, Aysun Genç, İsmihan Sunar, Sevgi Esra Özdemir, Derya Gökmen, Şebnem Ataman, Yesim Kurtais Aytür
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background:</h3> Axial Spondyloarthropathies (SpA) are chronic, systemic, inflammatory diseases which affect the axial skeleton, limit chest mobility and cause serious impairments in pulmonary functions. <h3>Objectives:</h3> In this cross-sectional study, as a new approach; diaphragm thickness is assessed by ultrasound in patients with axial SpA to determine possible relationships with pulmonary functions and disease activity. <h3>Methods:</h3> 49 axial SpA patients enrolled in the study were assessed by Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Bath Ankylosing Spondylitis Functional Index (BASFI), Bath Ankylosing Spondylitis Metrology index (BASMI), Erythrocyte Sedimentation Rate (ESR), serum C-Reactive Protein levels (CRP) and chest expansion (cm). 15 patients with lung diseases other than axial SpA pulmonary involvement, neuromuscular diseases, scoliosis and congenital chest deformity were excluded. Dynamic pulmonary function tests (PFT) and 6 min walk (6MW) tests were done and physical activity levels were evaluated by short form International Physical Activity Questionnaire (IPAQ). Diaphragm thickness was measured at end expiration (dtFRC) and deep inspiration (total lung capacity (dtTLC)). Thickness ratio (dtr: dtTLC/dtFRC) and thickness change (dtc: dtTLC-dtFRC) were calculated. <h3>Results:</h3> The descriptive data of the patients are presented in Table 1. Patients had a moderate disease activity and all the patients were category 1 (inactivity) according to IPAQ. Mean value of dtr and PFT seem to be within normal range according to population studies and dtr was significantly correlated with dynamic pulmonary function tests, yet negatively correlated with ESR (r: -0.466, p: 0.006). There was insignificant negative correlation with age, disease duration, BASDAI, BASFI, and BASMI. <h3>Conclusion:</h3> To the best of our knowledge, this is the first study evaluating the relation between diaphragm thickness and pulmonary functions with disease activity. Even though, there was no limitation in PFT and expected values were obtained in dtr, the negative correlation with ESR makes us think about the possible effect of disease activity on dtr. With these preliminary results, it is early to conclude, but dtr assessment may be complementary to PFT and early measures to improve diaphragm function with specific exercises should be implemented in patients with axial SpA. <h3>Reference</h3> [1] Carrillo-Esper R, Pérez-Calatayud ÁA, Arch-Tirado E, Díaz-Carrillo MA, Garrido-Aguirre E4, Tapia-Velazco R, Peña-Pérez CA, Espinoza-de Los Monteros I, Meza-Márquez JM, Flores-Rivera OI, Zepeda-Mendoza AD, de la Torre-León T. Standardization of Sonographic Diaphragm Thickness Evaluations in Healthy Volunteers Respir Care 2016;61(7):920-4 <h3>Disclosure of Interests:</h3> None declared
A Stromal Niche Defined by Expression of the Transcription Factor WT1 Mediates Programming and Homeostasis of Cavity-Resident Macrophages
Authors: Matthew B. Buechler, Ki-Wook Kim, Emily J. Onufer, Jesse W. Williams, Christine C. Little, Claudia X. Dominguez, Qingling Li, Wendy Sandoval, Jonathan E. Cooper, Charles A. Harris, Melissa R. Junttila, Gwendalyn J. Randolph, Shannon J. Turley
Journal: Immunity (2019)
PB2075 DIFFERENT IRON FORMULATIONS MIGHT CHANGE METHYLATION PATTERNS AND BONE MARROW MACROPHAGES POLARIZATION IN LOW RISK MYELODYSPLASTIC SYNDROMES (LRMDS)
Authors: G. Giordano, A. Lucchesi, M. magri, G. niro, M. gasperi
Journal: HemaSphere (2019)

Background: Iron status influences dna methylation. External factors as liposomes may switc-off bone marrow macrophages M1 polarization that is related to inflammatory status. These things are important in ineffective hemopoiesis in LRMDS. Aims: To see if sucromial iron and sodium ferrigluconate support in LRMDS shows different dna metilation patterns and macrophages polarization in bone marrow. Methods This study is a retrospective nonrandomized study. 45 patients with low risk refractory anemia receiving support with erythropoietin 40000 UI two times/week, b12 300 mg/day and folate 15 mg/day were divided in 3 group of 15 patients. Group A received i.v. sodium ferrigluconate 65 mg/day for 7 days/month, group B received sucrosomial iron tablets every other day, group C don’t received iron. No significant differences among median Hb level (9.5 g/dl), median age (65 yo), sex, karyotype, sEpo, ferritin, Tsat, IPSS, transfusion need, were in the three groups. Follow-up period was 6 months. The bone marrow M1 proinflammatory macrophages (iNOShigh/CD163low/CD14 + ) and M2 antiinflammatory (iNOSlow/CD163high/CD14-) were analyzed by cytofluorimetry and dna methylation with methylation-specific PCR. Bone marrow aspiration, M1 and M2 count, dna methylation, Hb level, transfusion need, ferritin and tsat level, CRP were recorded bimonthly. Results: All results were recorded at time 0, 2, 4 and 6 months. M1% was 1, 0.8, 0.7, 1 in group A, 1.2, 0.9, 0.6, 0.4 in group B, 0.8, 0.8, 0.9, 1 in group C. M2% was0.4, 0.5, 0.3, 0.4 in group A, 0.4, 0.5, 0.5,0.6 in group B, 0.3, 0.3, 0.4,0.3 in group C. % of methilated cytosine was 6% in group A, 3% in group B, 5% in group C. CRP (ng/ml) was 6, 8, 10, 12 in group A, 7, 5, 3, 2 in group B, 8, 6, 5, 5 in group C. Hb concentration (g/dl) was 9, 9.8, 11.5, 11.8 in group A, 9, 10.2, 11.5, 12.5 in group B, 9.5, 10, 10.5, 10.9 in group C. Transfusion need (units) was 2 in group A, 2 in group B, 2 in group C.Summary/Conclusion: In LRMDS iron support improves erythropoietin effectiveness and reduces dna methylation. Sucrosomial iron reduces macrophages M1 polarization and CRP, improving effective hemopoiesis.
OP0177 ASSOCIATION BETWEEN METABOLIC SYNDROME AND KNEE STRUCTURAL CHANGE ON MRI: A 10.7-YEAR FOLLOW-UP STUDY
Authors: Feng Pan, Jing Tian, Siti Maisarahmattap, Flavia Cicuttini, Graeme Jones
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Metabolic syndrome (MetS) has been suggested a crucial role in the pathophysiology of osteoarthritis (OA); however, currently none of studies have examined the associations between MetS and structural changes on MRI. <h3>Objectives</h3> To describe the associations between MetS and its components and strucutral changes on MRI including knee cartilage volume (CV) loss and bone marrow lesion (BML) change over 10.7 years. <h3>Methods</h3> Longitudinal data on 435 participants (mean age 61 years, 50% of females) from a population-based cohort study were analysed. Blood pressure, glucose, triglycerides, and high-density lipoprotein (HDL) were collected. MetS was defined based on the National Cholesterol Education Program-Adult Treatment Panel III criteria. MRI of the right knee was performed to measure CV and BML. Radiographic knee osteoarthritis (ROA) was assessed by X-ray. <h3>Results</h3> 32% of participants had MetS and 60% had ROA. In multivariable analysis, the following were independently associated with medial tibial CV loss [MetS: β=-0.30%; central obesity: β=-0.26%; low HDL: β=-0.26% per annum]. MetS, hypertriglyceridemia and low HDL were also associated with higher risk of BML size increase in the medial compartment [MetS: relative risk (RR) 1.72, 95%CI 1.22-2.43; Hypertriglyceridemia: RR 1.43, 95%CI 1.01-2.02; low HDL: RR 1.67, 95%CI 1.18-2.36]. After further adjustment for central obesity, MetS and low HDL remained significant with both medial tibial CV loss and BML size increase. The number of components of MetS correlated with greater CV loss and BML size increase (both P for trend &lt;0.05). There were no statistically significant associations in the lateral compartment. <h3>Conclusion</h3> MetS and low HDL are associated with medial compartment CV loss and BML worsening, suggesting that targeting MetS has the potential to prevent or slow structural change in knee osteoarthritis. <h3>Disclosure of Interests</h3> None declared
Publisher Correction: IFN-γ-independent immune markers of Mycobacterium tuberculosis exposure
Authors: Lenette L. Lu, Malisa T. Smith, Krystle K. Q. Yu, Corinne Luedemann, Todd J. Suscovich, Patricia S. Grace, Adam Cain, Wen Han Yu, Tanya R. McKitrick, Douglas Lauffenburger, Richard D. Cummings, Harriet Mayanja-Kizza, Thomas R. Hawn, W. Henry Boom, Catherine M. Stein, Sarah M. Fortune, Chetan Seshadri, Galit Alter
Journal: Nature Medicine (2019)

In the version of this article originally published, there was an error in the abstract. The word disease should not have been included in the sentence “These individuals were highly exposed to Mtb but tested negative disease by IFN-γ release assay and tuberculin skin test, ‘resisting’ development of classic LTBI”. The sentence should have been “These individuals were highly exposed to Mtb but tested negative by IFN-γ release assay and tuberculin skin test, ‘resisting’ development of classic LTBI.” The error has been corrected in the HTML and PDF versions of this article.
Adjuvant Chemotherapy plus Radiation for Locally Advanced Endometrial Cancer
Authors: Daniela Matei, Virginia Filiaci, Marcus E. Randall, David Mutch, Margaret M. Steinhoff, Paul A. DiSilvestro, Katherine M. Moxley, Yong M. Kim, Matthew A. Powell, David M. O’Malley, Nick M. Spirtos, William Small, Krishnansu S. Tewari, William E. Richards, John Nakayama, Ursula A. Matulonis, Helen Q. Huang, David S. Miller
Journal: New England Journal of Medicine (2019)

Stage III or IVA endometrial cancer carries a significant risk of systemic and locoregional recurrence.
AB0732 THE ASSOCIATION BETWEEN PERIODONTAL DISEASE AND RISK OF ANKYLOSING SPONDYLITIS: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Patompong Ungprasert, Karn Wijarnpreecha, Wisit Cheungpasitporn
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background:</h3> The association between periodontal disease and rheumatoid arthritis is well-recognized [1]. <i>Porphyromonas gingivalis</i> is probably the link between the two conditions. On the other hand, the data on the association between periodontal disease and ankylosing spondylitis are still relaitvely limited. <h3>Objectives:</h3> The current systematic review and meta-analysis was conducted with the aims to identify all publsihed studies that investiagted the risk of ankylosing spondylitis among patients with periodontal disease versus individuals without periodontal disease and summarize their results together to better characterize this relationship. <h3>Methods:</h3> Two investigators independently searched for published studies indexed in MEDLINE and EMBASE database from inception to October 2018 using the search strategy that included the terms for periodontal disease and ankylosing spondylitis. The inclusion criteria are as follows: (1) Case-control or cohort studies that compared the risk of ankylosing spondylitis between patients with periodontal disease versus individuals without periodontal disease (2) individuals without periodontal disease were used as comparators in cohort studies while individuals without ankylosing spondylitis were used as controls in case-control studies and (3) Effect estimates and 95% confidence intervals (CI) of the association of interest were reported. Point estimates and standard errors from each study were extracted and combined together using the random effect, generic inverse variance technique of DerSimonian and Laird. <h3>Results:</h3> Of 554 retrieved articles, a total of 7 case-control studies with 41,575 participants met the eligibility criteria and were included into the meta-analysis. The risk of ankylosing spondylitis among patients with periodontal disease was significantly higher than individuals without periodontal disease with the pooled odds ratio of 2.16 (95% CI, 1.48–3.16). The statistical heterogeneity was low with an I<sup>2</sup> of 45%. The forest plot of this meta-analysis is shown as figure 1. Funnel plot was created for evaluation of publication bias. The plot was relatively symmetric which did not suggest the presence of publication bias in favor of studies with positive results. <h3>Conclusion:</h3> A significantly increased risk of ankylosing spondylitis among patients with periodontal disease was demonstrated in this study. This observation may provide more understanding of the pathogenesis of this chronic inflammatory arthritis. <h3>References</h3> [1] Leech MT, Bartold PM. The association between rheumatoid arthritis and periodontitis. Best Pract Res Clin Rheumatol2015;29:189-201. <h3>Disclosure of interests:</h3> None declared
SP0120 PERSONALISED TREATMENT FOR FIBROMYALGIA; THE IMPACT OF COMORBIDITIES
Authors: Serge Perrot
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background:</h3> The heterogeneity of the clinical presentation, the co-morbidities and the pathophysiologic mechanisms associated with fibromyalgia (FM), and the modest results on average for any therapy, call for a more individualized management strategy. How to develop personalized treatment? Individualized treatment can be developed on the basis of subgrouping of patients according to associated conditions and co-morbidities (mental health problems, chronic overlapping pain conditions, other somatic diseases) or on disease severity. Personalizing treatment needs to classify FM in specific subgroups. It can be proposed on the basis of clinical assessment (eg, degree of daily functioning), on co-morbidities or on dedicated questionnaires. Lastly, shared decision-making regarding treatment options can be directed according to patient preferences, co-morbidities, and availability in various health care settings. What is currently recommended as a personalized approach? The European League Against Rheumatism guidelines recommend a tailored approach directed by FM key symptoms (pain, sleep disorders, fatigue, depression, disability), whereas the German guidelines recommend management tailored to disease severity, with mild disease not requiring any specific treatment, and more severe disease requiring multicomponent therapy (combination of drug treatment with aerobic exercise and psychological treatments). When indicated, treatments should follow a stepwise approach beginning with easily available therapies such as aerobic exercise and amitriptyline. We will present how successful application of a tailored treatment approach that is informed by individual patient characteristics should improve outcome of FM. <h3>Key points:</h3> This presentation will presents suggestions for an individualized treatment strategy for FM patients on the basis of symptoms, disease severity and co-morbidities. <h3>Disclosure of Interests:</h3> None declared
AB1372-HPR THE INVESTIGATION OF THE RELATIONSHIP BETWEEN PSYCHOSOCIAL STATUS OF CHILDREN WITH JIA AND PARENTS
Authors: Aykut Özçadırcı, Aybüke Seven, Gamze Arın, Yasemin Özel Aslıyüce, Fatma Birgül Kumbaroğlu, Nur Banu Karaca, Selcan Demir, Yelda Bilginer, Edibe Ünal, Seza Özen
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Juvenile idiopathic arthritis (JIA) is a heterogeneous group of diseases characterized by unknown origin arthritis that begins before the age of 16 years (1). Therefore, families are often affected by the disease of children. Parents and children often disagree with assessing different aspects of disease activity in JIA (2). <h3>Objectives</h3> The aim of this study is to investigate the relationship between children with JIA and their parents' psychosocial status. <h3>Methods</h3> A total of 345 children with JIA and their parents were included in the study (n = 690). Juvenile Arthritis Biopsychosocial Questionnaire (JAB-Q) was administered to the subjects (3). The psychosocial status of the individuals was evaluated by this questionnaires. <h3>Results</h3> The mean age of children included in the study (n = 345) was 12.32 3.76 years. While the median value of the Child Psychosocial score was 10 (min: 0 max: 38), the median value of the Parent Psychosocial score was 6 (min: 0 max: 20). Correlation coefficients and statistical significance were calculated by using the Pearson test. A positive low correlation was found between the psychosocial status of the child and parents (r = 0.273, p &lt;0.001) (Table 1). <h3>Conclusion</h3> It was observed that there was a low relationship between the psychosocial status of children and parents. It was concluded that child and parents psychosocial status may be affected by other variables such as high disease activity, school attendance. Further studies are needed in this area including different variables and interaction psychosocial status. <h3>References</h3> [1] Prakken, B., Albani, S., &amp; Martini, A. (2011). Juvenile idiopathic arthritis. The Lancet, 377(9783), 2138-2149. [2] Garcia-Munitis P, Bandeira M, Pistorio A, Magni-Manzoni S, Ruperto N, Schivo A, et al. Level of agreement between children, parents, and physicians in rating pain intensity in juvenile idiopathic arthritis. Arthritis Rheum 2006;55:177-83. <h3>Disclosure of Interests</h3> None declared
Abstract 19618: Lower Aortic Valve Calcification Volume Index Paradoxically Predicts Heart Failure Hospitalization After Successful Transfemoral Transcatheter Aortic Valve Implantation
Authors: Yutaka Konami, Tomohiro Sakamoto, Hiroto Suzuyama, Eiji Taguchi, Youko Horibata, Nobuhisa Hagiwara, Koichi Nakao
Journal: Circulation (2017)

Introduction: Transcatheter aortic valve implantation (TAVI) is now a standard treatment for patients with inoperable severe aortic stenosis (AS). However, less information is available for predict...
A FULLY Human Bispecific Antibody Functionally Rescues Factor VIII Deficiency EX VIVO
Authors: Wei Wang, John Blackwood, Roberto Magliozzi, Leonardo Moraes, Jeffrey Hollins, Alessandro Sinopoli, Bertie Chi, Laura Mitchell, Chris Sellick, Claire Pearce, Igor Theurl, Volker Germaschewski, Jacob Galson, Luca Badiali, Karen Dickson, Allan Bradley, E-Chiang Lee
Journal: Blood (2019)