tbuckley/cabot-search huggingface dataset:
Bmi-1 extends the replicative life span of normal human oral keratinocytes independently of p16INK4A
Authors: Mo K. Kang, Reuben H. Kim, Zin M. Oo, Ki-Hyuk Shin, No-Hee Park
Journal: Cancer Research (2005)

2826  Normal human oral keratinocytes (NHOK) undergo a limited number of replication in vitro and enter replicative senescence without spontaneous immortalization. Escape from senescence is therefore one of the earliest steps in cellular immortalization and tumorigenesis. The current study was undertaken to investigate the role of polycomb group (PcG) oncogene, Bmi-1, in control of replicative life span of NHOK. Secondary NHOK culture was infected with retroviral vector expressing full-length Bmi-1 or empty vector and was maintained in serial subcultures after selection with puromycin. Parental NHOK and those infected with the empty vector senesced after 16 population doublings (PDs), while the Bmi-1 expressing NHOK continued replication beyond 44 PDs, after which time the cells demonstrated features resembling senescent NHOK. Parental NHOK showed enhanced expression of p16INK4A during senescence, and p16INK4A expression remained at the elevated level in exponentially replicating NHOK expressing Bmi-1. During the Bmi-1-induced extended life span, telomerase activity was not detected, and the cells replicated with notable telomere shortening. Also, super infection of Bmi-1 expressing NHOK with a retroviral vector expressing E6 but not E7 oncoprotein of human papillomavirus (HPV) type 16 further enhanced their replicative life span beyond that induced by Bmi-1 alone. These results indicate that Bmi-1 extends the life span of NHOK independently of the p16INK4A level but cannot immortalize them. This study was supported in part by the grants DE 15316 and DE 14147 from NIDCR/NIH.
Piercing technique for recanalization of pancreaticojejunal obstruction through the endoscopic ultrasound-guided pancreatic duct drainage route
Authors: Tatsuya Ishii, Tsuyoshi Hayashi, Kuniyuki Takahashi, Toshifumi Kin, Akio Katanuma
Journal: Endoscopy (2020)

Endoscopic ultrasound-guided pancreatic duct intervention is considered to be the most difficult procedure and requires various techniques to achieve successful treatment, particularly in patients who have undergone pancreatectomy [1]. We report a case of successful recanalization of a complete anastomotic obstruction after pancreaticojejunostomy using a piercing technique ([Video 1]).
Abstract 15298: Ischemic Postconditioning With HDL Protects Mitochondrial Complex I Activity
Authors: Joaquim B Oliveira, Isabella Bonilha, Estela Busanello, Ana Carolina Marques Marques, José Carlos Lima, Vitor W Virginio, Michele Santana, Orlando Petrucci, Anibal Vercesi, Andrei Sposito
Journal: Circulation (2018)

Introduction: Myocardial reperfusion injury underlies half of final infarct size following myocardial infarction and, so far, no effective therapy has been demonstrated. Modulation of mitochondrial...
Methylation in pericytes after acute injury promotes chronic kidney disease
Authors: Yu-Hsiang Chou, Szu-Yu Pan, Yu-Han Shao, Hong-Mou Shih, Shi-Yao Wei, Chun-Fu Lai, Wen-Chih Chiang, Claudia Schrimpf, Kai-Chien Yang, Liang-Chuan Lai, Yung-Ming Chen, Tzong-Shinn Chu, Shuei-Liong Lin
Journal: Journal of Clinical Investigation (2020)

The origin and fate of renal myofibroblasts is not clear after acute kidney injury (AKI). Here, we demonstrate that myofibroblasts were activated from quiescent pericytes (qPericytes) and the cell numbers increased after ischemia/reperfusion injury-induced AKI (IRI-AKI). Myofibroblasts underwent apoptosis during renal recovery but one-fifth of them survived in the recovered kidneys on day 28 after IRI-AKI and their cell numbers increased again after day 56. Microarray data showed the distinctive gene expression patterns of qPericytes, activated pericytes (aPericytes, myofibroblasts), and inactivated pericytes (iPericytes) isolated from kidneys before, on day 7, and on day 28 after IRI-AKI. Hypermethylation of the Acta2 repressor Ybx2 during IRI-AKI resulted in epigenetic modification of iPericytes to promote the transition to chronic kidney disease (CKD) and aggravated fibrogenesis induced by a second AKI induced by adenine. Mechanistically, transforming growth factor-β1 decreased the binding of YBX2 to the promoter of Acta2 and induced Ybx2 hypermethylation, thereby increasing α-smooth muscle actin expression in aPericytes. Demethylation by 5-azacytidine recovered the microvascular stabilizing function of aPericytes, reversed the profibrotic property of iPericytes, prevented AKI-CKD transition, and attenuated fibrogenesis induced by a second adenine-AKI. In conclusion, intervention to erase hypermethylation of pericytes after AKI provides a strategy to stop the transition to CKD.
Maternal Health Equity and Justice for Pregnant Women Who Experience Incarceration
Authors: Andrea Knittel, Carolyn Sufrin
Journal: JAMA Network Open (2020)

Alison Carter Ramirez, MD; Jessica Liauw, MD; Alice Cavanagh, MA; Dustin Costescu, MD, MS; Laura Holder, MSc; Hong Lu, PhD; Fiona G. Kouyoumdjian, MD, MPH, PhD
Covid-19: 700 000 patients could be left unable to see a GP face to face, charity warns
Authors: Abi Rimmer
Journal: BMJ (2020)

Covid-19: 700 000 patients could be left unable to see a GP face to face, charity warns Abi RimmerMore than 700 000 patients in England may be unable to get to face-to-face appointments because their GPs are at high risk from covid-19, a report from the Health Foundation has said.Research conducted by the charity found that 7.9% (3632) of all GPs and a third (209 of 639) of singlehanded GPs were at high risk of death from covid-19. 1 1 Health Foundation.How might covid-19 affect the number of GPs available to see patients in England? 6 Aug 2020.https://www.health.org.
Abstract IA26: The need for evidence-based survivorship care
Authors: Leontien Kremer
Journal: Cancer Research (2020)

Abstract The 5-year survival for children with cancer has increased enormously. However, years after treatment, childhood cancer survivors are at high risk for developing health and psychosocial long-term side effects due to their previous cancer treatment. The impacts on morbidity, mortality, and society are significant. These impacts can be reduced by optimal long-term follow-up of survivors. Survivorship care that is focused on the detection of treatable disease at an early phase will improve quality of life of survivors by timely interventions. The International Guideline Harmonisation Group (ighg.org) developed evidence-based guidelines for many late effects. However, implementing these guidelines has proven challenging. The availability of follow-up care for adult survivors varies considerably, and many clinics do not offer survivorship care for adult survivors of childhood cancer. High-quality survivorship care requires a multidisciplinary care infrastructure, implementation of guidelines, and development of a survivorship care plan with a detailed summary of treatment and specific knowledge on late effects. This presentation will focus on the translation of knowledge to guidelines and the need for survivorship care. Citation Format: Leontien Kremer. The need for evidence-based survivorship care [abstract]. In: Proceedings of the AACR Special Conference on the Advances in Pediatric Cancer Research; 2019 Sep 17-20; Montreal, QC, Canada. Philadelphia (PA): AACR; Cancer Res 2020;80(14 Suppl):Abstract nr IA26.
Response to: ‘Exacerbation of immune thrombocytopenia triggered by COVID-19 in patients with systemic lupus erythematosus’ by Kondo et al
Authors: Alexis Mathian, Zahir Amoura
Journal: Annals of the Rheumatic Diseases (2020)

<i>Staphylococcus aureus</i> expresses a highly diversified arsenal of immune evasion proteins, many of which target the complement system. The extracellular fibrinogen-binding protein (Efb) and the Efb homologous protein (Ehp) have previously been demonstrated to bind to C3 and inhibit complement activation and amplification. In this study we present the first evidence that Efb and Ehp are also capable of inhibiting the interaction of C3d with complement receptor 2 (CR2), which plays an important role in B cell activation and maturation. The C-terminal domain of Efb efficiently blocked this interaction both in surface plasmon resonance-based competition studies and cellular assays and prevented the CR2-mediated stimulation of B cells. Furthermore, analyses of the available structural data were consistent with a molecular mechanism that reflects both steric and electrostatic effects on the C3d-CR2 interaction. Our study therefore suggests that <i>S. aureus</i> may disrupt both the innate and adaptive immune responses with a single protein module.
Expression Levels of PD-1 on CD8+ T Cells in Chronic Myeloid Leukemia with and without BCR-ABL1 kinase Mutation
Authors: Min Young Lee, Chan-jeoung Park, Young-Uk Cho, Eunkyoung You, Seongsoo Jang, Chang Ahn Seol, Eul-Ju Seo, Jung-Hee Lee
Journal: Blood (2017)
Reducing Inpatient Hypoglycemia in the General Wards Using Real-time Continuous Glucose Monitoring: The Glucose Telemetry System, a Randomized Clinical Trial
Authors: Lakshmi G. Singh, Medha Satyarengga, Isabel Marcano, William H. Scott, Lillian F. Pinault, Zhaoyong Feng, John D. Sorkin, Guillermo E. Umpierrez, Elias K. Spanakis
Journal: Diabetes Care (2020)

Use of real-time continuous glucose monitoring (RT-CGM) systems in the inpatient setting is considered investigational. The objective of this study was to evaluate whether RT-CGM, using the glucose telemetry system (GTS), can prevent hypoglycemia in the general wards.In a randomized clinical trial, insulin-treated patients with type 2 diabetes at high risk for hypoglycemia were recruited. Participants were randomized to RT-CGM/GTS or point-of-care (POC) blood glucose testing. The primary outcome was difference in inpatient hypoglycemia.Seventy-two participants were included in this interim analysis, 36 in the RT-CGM/GTS group and 36 in the POC group. The RT-CGM/GTS group experienced fewer hypoglycemic events (<70 mg/dL) per patient (0.67 [95% CI 0.34-1.30] vs. 1.69 [1.11-2.58], P = 0.024), fewer clinically significant hypoglycemic events (<54 mg/dL) per patient (0.08 [0.03-0.26] vs. 0.75 [0.51-1.09], P = 0.003), and a lower percentage of time spent below range <70 mg/dL (0.40% [0.18-0.92%] vs. 1.88% [1.26-2.81%], P = 0.002) and <54 mg/dL (0.05% [0.01-0.43%] vs. 0.82% [0.47-1.43%], P = 0.017) compared with the POC group. No differences in nocturnal hypoglycemia, time in range 70-180 mg/dL, and time above range >180-250 mg/dL and >250 mg/dL were found between the groups. The RT-CGM/GTS group had no prolonged hypoglycemia compared with 0.20 episodes <54 mg/dL and 0.40 episodes <70 mg/dL per patient in the POC group.RT-CGM/GTS can decrease hypoglycemia among hospitalized high-risk insulin-treated patients with type 2 diabetes.
The Utility of Perforated Appendix Rate as a Proxy for Timely Access to Care
Authors: John W. Scott, Andrew P. Loehrer
Journal: JAMA Surgery (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
AB0200 SHORT AND MID-TERM OUTCOME OF FOREFOOT SURGERY FOR PATIENTS WITH RHEUMATOID ARTHRITIS
Authors: R. Kurose
Journal: Annals of the Rheumatic Diseases (2020)

Background: Patients with rheumatoid arthritis (RA) have frequently painful foot deformities. These deformities including hallux valgus, dorsal dislocation of the metatarsophalangeal (MTP) joints and hammer toe deformity of the lesser toes are associated with disability in daily activities. Objectives: The aim of this study is to investigate short and mid-term outcome of forefoot surgery for patients with RA. Methods: We investigated 28 patients with 39 foots who underwent forefoot surgery between January 2010 and December 2018 and followed for more than one year after surgery. Swanson implant arthroplasty or metatarsal osteotomy was performed for big toe, and shortening oblique osteotomy (SOO) was performed for the II-V toes. Examination items were as follows; plain X-ray changes in the angle of hallux valgus (HVA), the angle between the first and second metatarsal bones (M1/2), and the angle between the first and fifth metatarsal bones (M1/5) after surgery. The progress of painful hyperkeratosis and bunion, Japanese Society for Surgery of the foot standard rating system (JSSF scale), the recurrence of deformity and bone fusion, complications, and patient satisfaction were also examined. Results: The patients followed were 24 women with 34 feet and 4 men with 5 feet, and average age at the time of surgery was 66.1 years. HVA improved from average of 39.7 degrees to 20.2 degrees one month after surgery, but increased slightly to 23.5 degrees one year after surgery. Painful hyperkeratosis and bunion tended to disappear one month after surgery, and JSSF scale significantly improved at the final observation. One year after surgery, the bone fusion rate of big toe was 100%, but 7.8% of the metatarsal bone underwent SOO had non-union. The complications after surgery were one infection at the surgical site, one necrosis at the tip of the toe, and one infection of Swanson implant. Patient satisfaction at the final observation was high Conclusion: Forefoot surgery for RA has problems such as recurrence, non-union, and infections, but it is considered to be a useful treatment because that pain by hyperkeratosis and bunion disappears and ADL improves. References: [1]Sung IH, Sung YK, Huh DR, Kim SJ. A comparative study on the results of the modified Ludloff osteotomy for hallux valgus deformities with minimal erosion of the metatarsophalangeal joints in rheumatoid patients versus non-rheumatoid patients.. Mod Rheumatol 2015; 25(5): 694-700. Disclosure of Interests: None declared
Abstract 14006: Sex Differences of Coronary Physiology Can Be Justified by Fractional Myocardial Mass
Authors: Joon Ho Ahn, Hyung Yoon Kim, Kye Hun Kim, Jae Yeong Cho, Hyun Ju Yoon, Hyukjin Park, Jin-Ho Choi
Journal: Circulation (2019)

Introduction: The gender difference in the anatomical-physiological relationship may elucidate the gender difference in the clinical presentation of coronary artery disease. Hypothesis: We hypothes...
Novel double endoscopic ultrasound-guided hepaticogastrostomy for two-hole benign anastomotic stenosis with difficult gastrointestinal approach
Authors: Hidetoshi Kitamura, Susumu Hijioka, Yuta Maruki, Akihiro Ohba, Yoshikuni Nagashio, Takuji Okusaka, Yutaka Saito
Journal: Endoscopy (2020)

Enteroscopy is useful for approaching anastomotic stenoses; however, in surgically altered anatomy, success rates of 70 % – 80 % have been reported [1]. Alternatively, endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) may be acceptable [2]. Usually, one EUS-HGS is performed; however, double EUS-HGS is required for two-hole anastomotic stenosis to resolve symptoms. We report the first case of successful double EUS-HGS for two-hole benign anastomotic stenosis.
Increased Hepatic ACE2 Expression in NAFL and Diabetes—A Risk for COVID-19 Patients?
Authors: Jelena Soldo, Martin Heni, Alfred Königsrainer, Hans-Ulrich Häring, Andreas L. Birkenfeld, Andreas Peter
Journal: Diabetes Care (2020)

The current pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced disease (coronavirus disease 2019 [COVID-19]) is a major global threat.Although most infected patients have a mild course, a relevant proportion of approximately 20% develop a severe and often lethal condition.Recent findings demonstrate that COVID-19 is a multiorgan disease.In addition to the characteristic pneumonia, it affects heart, kidney, pancreas, the coagulation system, and liver.Major risk factors for severe COVID-19 with multiorgan complications include male sex, older age (.65 years), cardiovascular and chronic lung disease, and especially diabetes (1,2).However, it is still unclear why these conditions predispose to a severe course of COVID-19.To enter the target cell, SARS-CoV-2 requires the binding of its spike protein to the angiotensin-converting enzyme 2 (ACE2) receptor (3).The entry is additionally facilitated by subsequent priming and cleavage of the spike protein through proteases such as transmembrane serine protease 2 (TPMRSS2) and furin (3).While the ACE2 receptor is mainly expressed in heart, lungs, endothelial cells, kidney, and the gastrointestinal tract, expression has also been detected in cholangiocytes and
Integrated pipeline for the accelerated discovery of antiviral antibody therapeutics
Authors: Pavlo Gilchuk, Robin G. Bombardi, Jesse H. Erasmus, Qing Tan, Rachel Nargi, Cinque Soto, Peter Abbink, Todd J. Suscovich, Lorellin A. Durnell, Amit Khandhar, Jacob Archer, Jenny Liang, Mallorie E. Fouch, Edgar Davidson, Benjamin J. Doranz, Taylor Jones, Elise Larson, Stacey Ertel, Brian Granger, Jasmine Fuerte-Stone, Vicky Roy, Thomas Broge, Thomas C. Linnekin, Caitlyn H. Linde, Matthew J. Gorman, Joseph Nkolola, Galit Alter, Steven G. Reed, Dan H. Barouch, Michael S. Diamond, James E. Crowe, Neal Van Hoeven, Larissa B. Thackray, Robert H. Carnahan
Journal: Nature Biomedical Engineering (2020)
Nephron Progenitor Maintenance Is Controlled through Fibroblast Growth Factors and Sprouty1 Interaction
Authors: Sung-Ho Huh, Ligyeom Ha, Hee-Seong Jang
Journal: Journal of the American Society of Nephrology (2020)

Significance Statement Nephrons are derived from nephron progenitor cells. Nephron progenitors are depleted during kidney development, which makes the kidney unable to regenerate nephrons. Therefore, understanding the signaling molecules that regulate nephron progenitor cell generation and maintenance is of great interest for kidney regeneration. Sprouty1 regulates nephron progenitor maintenance by inhibiting Fibroblast growth factor (FGF) signaling. Deletion of Sprouty1 rescues renal agenesis and nephron progenitor depletion in Fgf9/20 loss-of-function kidneys. Deletion of one copy of Fgf8 further decreases FGF signaling, which blocks kidneys’ response to Sprouty1 , resulting in failure of nephron progenitor maintenance. This study thus identifies the reciprocal functioning of FGF-Sprouty1 signaling during nephron progenitor development. Background Nephron progenitor cells (NPCs) give rise to all segments of functional nephrons and are of great interest due to their potential as a source for novel treatment strategies for kidney disease. Fibroblast growth factor (FGF) signaling plays pivotal roles in generating and maintaining NPCs during kidney development, but little is known about the molecule(s) regulating FGF signaling during nephron development. Sprouty 1 (SPRY1) is an antagonist of receptor tyrosine kinases. Although SPRY1 antagonizes Ret-GDNF signaling, which modulates renal branching, its role in NPCs is not known. Methods Spry1 , Fgf9 , and Fgf20 compound mutant animals were used to evaluate kidney phenotypes in mice to understand whether SPRY1 modulates FGF signaling in NPCs and whether FGF8 functions with FGF9 and FGF20 in maintaining NPCs. Results Loss of one copy of Spry1 counters effects of the loss of Fgf9 and Fgf20 , rescuing bilateral renal agenesis premature NPC differentiation, NPC proliferation, and cell death defects. In the absence of SPRY1, FGF9, and FGF20, another FGF ligand, FGF8, promotes nephrogenesis. Deleting both Fgf8 and Fgf20 results in kidney agenesis, defects in NPC proliferation, and cell death. Deleting one copy of Fgf8 reversed the effect of deleting one copy of Spry1 , which rescued the renal agenesis due to loss of Fgf9 and Fgf20 . Conclusions SPRY1 expressed in NPCs modulates the activity of FGF signaling and regulates NPC stemness. These findings indicate the importance of the balance between positive and negative signals during NPC maintenance.
Induction of ferroptosis-like cell death of eosinophils exerts synergistic effects with glucocorticoids in allergic airway inflammation
Authors: Yanping Wu, Haixia Chen, Nanxia Xuan, Lingren Zhou, Yinfang Wu, Chen Zhu, Miao Li, Qingyu Weng, Jiaxin Shen, Hao Zhang, Bin Zhang, Fen Lan, Lixia Xia, Xuefang Xiong, Zhouyang Li, Yun Zhao, Mindan Wu, Songmin Ying, Wen Li, Huahao Shen, Zhihua Chen
Journal: Thorax (2020)

Introduction Eosinophils are critical in allergic disorders, and promoting eosinophil death effectively attenuates allergic airway inflammation. Ferroptosis is a recently described novel form of cell death; however, little is known about ferroptosis in eosinophils and related diseases. This study aimed to investigate the effects of ferroptosis-inducing agents (FINs) on eosinophil death and allergic airway inflammation, and to explore their potential synergistic effect with glucocorticoids (GCs). Methods Eosinophils isolated from the peripheral blood of humans or mice were incubated with FINs, and eosinophil ferroptosis was assessed. The in vivo effects of FINs alone or in combination with dexamethasone (DXMS) were examined in a mouse model of allergic airway inflammation. Bronchoalveolar lavage fluid and lung tissue were collected to examine airway inflammation. Results Treatment with FINs time and dose dependency induced cell death in human and mouse eosinophils. Interestingly, FINs induced non-canonical ferroptosis in eosinophils, which generated morphological characteristics unique to ferroptosis and was iron dependent but was independent of lipid peroxidation. The antioxidants glutathione and N-acetylcysteine significantly attenuated FIN-induced cell death. Treatment with FINs triggered eosinophil death in vivo and eventually relieved eosinophilic airway inflammation in mice. Furthermore, FINs exerted a synergistic effect with DXMS to induce eosinophil death in vitro and to alleviate allergic airway inflammation in vivo. Conclusions FINs induced ferroptosis-like cell death of eosinophils, suggesting their use as a promising therapeutic strategy for eosinophilic airway inflammation, especially due to the advantage of their synergy with GCs in the treatment of allergic disorders.