tbuckley/cabot-search huggingface dataset:
Molecular Imaging of Chimeric Antigen Receptor T Cells by ICOS-ImmunoPET
Authors: Federico Simonetta, Israt S. Alam, Juliane K. Lohmeyer, Bita Sahaf, Zinaida Good, Weiyu Chen, Zunyu Xiao, Toshihito Hirai, Lukas Scheller, Pujan Engels, Ophir Vermesh, Elise Robinson, Tom Haywood, Ataya Sathirachinda, Jeanette Baker, Meena B. Malipatlolla, Liora M. Schultz, Jay Y. Spiegel, Jason T. Lee, David B. Miklos, Crystal L. Mackall, Sanjiv S. Gambhir, Robert S. Negrin
Journal: Clinical Cancer Research (2020)

Abstract Purpose: Immunomonitoring of chimeric antigen receptor (CAR) T cells relies primarily on their quantification in the peripheral blood, which inadequately quantifies their biodistribution and activation status in the tissues. Noninvasive molecular imaging of CAR T cells by PET is a promising approach with the ability to provide spatial, temporal, and functional information. Reported strategies rely on the incorporation of reporter transgenes or ex vivo biolabeling, significantly limiting the application of CAR T-cell molecular imaging. In this study, we assessed the ability of antibody-based PET (immunoPET) to noninvasively visualize CAR T cells. Experimental Design: After analyzing human CAR T cells in vitro and ex vivo from patient samples to identify candidate targets for immunoPET, we employed a syngeneic, orthotopic murine tumor model of lymphoma to assess the feasibility of in vivo tracking of CAR T cells by immunoPET using the 89Zr-DFO-anti-ICOS tracer, which we have previously reported. Results: Analysis of human CD19-CAR T cells during activation identified the Inducible T-cell COStimulator (ICOS) as a potential target for immunoPET. In a preclinical tumor model, 89Zr-DFO-ICOS mAb PET-CT imaging detected significantly higher signal in specific bone marrow–containing skeletal sites of CAR T-cell–treated mice compared with controls. Importantly, administration of ICOS-targeting antibodies at tracer doses did not interfere with CAR T-cell persistence and function. Conclusions: This study highlights the potential of ICOS-immunoPET imaging for monitoring of CAR T-cell therapy, a strategy readily applicable to both commercially available and investigational CAR T cells. See related commentary by Volpe et al., p. 911
Gene array analysis and the liver
Authors: Nicholas A. Shackel, Mark D. Gorrell, Geoffrey W. McCaughan
Journal: Hepatology (2002)

Functional genomics methods promise a previously unparalleled high-throughput examination of intrahepatic gene expression. Profiling transcriptomes as well as examining the coordinate expression of many genes in diverse pathobiologic pathways is now pssible with techniques such as gene array analysis. However, the nature of the hepatic transcriptome, limitations of the functional genomics methokologies used, and analysis of the data generated are often poorly understood. Further, the use of replicates during analysis and the downstream confirmation of results using a supplemental methokology are important but frequently neglected aspects of experimenta design. This review presents an overview of functional genomics methodoogies in the organ-specific context of studying intrahepatic gene expression. The focus of the review is the most widely used functional genomic methodology, gene array analysis. The nature of the hepatic transcriptome is discussed, together with a summary of gene array analysis methodologies. Analysis of data generated by gene array analysis, determination of intrahepatic cellular origin of transcript expression, and studies of intrahepatic gene expression using gene array analysis are also reviewed. Finally, the importance of a coordinate experimental approach examining both liver protein and mRNA expression is presented. (Hepatology 2002;36: 1313-1325).
Additional Concerns Regarding Children With Coronavirus Disease 2019—Reply
Authors: Riccardo Castagnoli, Amelia Licari, Gian Luigi Marseglia
Journal: JAMA Pediatrics (2020)

Riccardo Castagnoli, MD; Martina Votto, MD; Amelia Licari, MD; Ilaria Brambilla, MD, PhD; Raffaele Bruno, MD; Stefano Perlini, MD; Francesca Rovida, PhD; Fausto Baldanti, MD; Gian Luigi Marseglia, MD
TCT CONNECT-333 German Multicenter Experience With a New Leaflet-Based Transcatheter Mitral Valve Repair System for Mitral Regurgitation
Authors: Victor Mauri, Matthias Riebisch, Tobias Ruf, Muhammed Gerçek, Patrick Horn, Christina Grothusen, Michael Mehr, Christoph Mues, Niklas Boeder, Felix Kreidel, Kai Friedrichs, Ralf Westenfeld, Daniel Braun, Can Öztürk, Stephan Baldus, Tienush Rassaf, Holger Thiele, Georg Nickenig, Jörg Hausleiter, Helge Möllmann, Volker Rudolph, Ralph Stephan von Bardeleben, Holger Nef, Peter Luedike, Philipp Lurz, Roman Pfister
Journal: Journal of the American College of Cardiology (2020)
Overall survival with 3 or 6 months of adjuvant chemotherapy in Italian TOSCA phase 3 randomised trial
Authors: F. Petrelli, E. Rulli, R. Labianca, S. Lonardi, G. Rosati, K. Dotti, M. Ronzoni, N. Pella, V. Pusceddu, M. Banzi, M.G. Zampino, M. Yasmina, P. Marchetti, M. Cantore, A. Zaniboni, L. Rimassa, L. Ciuffreda, D. Ferrari, V. Zagonel, E. Maiello, A. Sobrero
Journal: Annals of Oncology (2020)
Doxycycline: Coroner calls for MHRA to review side effects after student jumped from plane
Authors: Clare Dyer
Journal: BMJ (2020)

A senior coroner has demanded action from the Medicines and Healthcare Products Regulatory Agency after a student who had taken an antimalarial drug jumped from a plane to her death.

Alana Cutland, 19, a natural sciences student at Cambridge University, was doing an internship in Madagascar when she had several attacks of paranoia.

She had spoken to her parents and was about to fly home to the UK after cutting her internship short in July 2019 when she travelled in the Cessna light aircraft. She opened one of the plane’s doors and, despite attempts by the pilot and the other passenger to restrain her, …
FRI0502 SEASONAL CLUSTERING OF ACUTE SARCOIDOSIS IN GERMANY AND ASSOCIATIONS WITH AIR POLLUTION
Authors: P. Rustler, D. Schindler, R. Voll, F. Kollert
Journal: Annals of the Rheumatic Diseases (2020)

Background: Sarcoidosis is a multisystemic granulomatous disorder of unknown origin. The central role of macrophages and granuloma formation, the predominant involvement of lung and skin, and certain risk populations (e.g. firefighters 1, 2 ) might be explained by causative airborne antigen(s) 3 . Whether air pollution is involved in pathogenesis and seasonal clustering of sarcoidosis is uncertain. Objectives: This study has been set to analyze seasonal clustering of acute sarcoidosis and associations to air pollution. Methods: Patients with acute sarcoidosis, defined by bihilar lymphadenopathy, ankle swelling, and/or erythema nodosum plus physician’s diagnosis,were included in this retrospective study. Disease onset (seasonal clustering) and associations to air pollution (particulate matter (PM 10 ) and nitrogen dixoide (NO 2 )) were analyzed. Google Trends queries were conducted to address seasonal clustering on a global scale. Results: A total of 185 patients with acute sarcoidosis were included; 48.7 % of the enrolled patients were female and Löfgren triad was complete in 73.5 % of patients. Acute sarcoidosis clustered from December to June in West Germany (p&lt;0.005, Kendall τ=-0.68), peaking in January (17.8 % of cases) and in the first third of the year (54.5 %). Mean PM 10 values clustered from December to April with values between 15 and 40 µg/m 3 . NO 2 levels were measured highest from November to March (45 µg/m 3 ) and lowest between April and August (25 µg/m 3 ). Elevated air pollution markers (PM 10 and NO 2 ) were associated with higher monthly incidence rates of acute sarcoidosis (Cross correlation coefficient ranging between 0.7 -0.8). Google Trends analysis yielded seasonal clustering (p&lt;0.005, Kendall τ = -0.64) in winter and spring months on the northern hemisphere. Conclusion: In Central Europe acute sarcoidosis peaks in winter and spring months (December until March) shortly after PM 10 and NO 2 maxima are reached. Whether components of particulate matter might be involved in the pathogenesis of sarcoidosis has to be elucidated by further studies. References: [1] Prezant DJ, Dhala A, Goldstein A, Janus D, Ortiz F, Aldrich TK, et al. The incidence, prevalence, and severity of sarcoidosis in New York City firefighters. Chest. 1999; [2] Webber MP, Yip J, Zeig-Owens R, Moir W, Ungprasert P, Crowson CS, et al. Post-9/11 sarcoidosis in WTC-exposed firefighters and emergency medical service workers. Respir Med [Internet]. 2017;132:232–7. [3] Newman LS, Rose CS, Bresnitz EA, Rossman MD, Barnard J, Frederick M, et al. A case control etiologic study of sarcoidosis: Environmental and occupational risk factors. Am J Respir Crit Care Med. 2004;170(12):1324–30. Disclosure of Interests: Philipp Rustler: None declared, Dirk Schindler: None declared, Reinhard Voll: None declared, Florian Kollert Employee of: Novartis
Salvage antegrade endoscopic ultrasound-guided pancreatic guidewire placement allowing subsequent double-balloon ERCP
Authors: Rintaro Fukuda, Tomotaka Saito, Yousuke Nakai, Atsuo Yamada, Hirofumi Kogure, Kazuhiko Koike
Journal: Endoscopy (2020)

During endoscopic retrograde cholangiopancreatography (ERCP), biliary cannulation is still challenging in patients with anatomical variations, such as an intradiverticular ampulla or surgically altered anatomy [1] [2]. While the double-guidewire (DGW) technique is one of the possible rescue techniques [3] [4], pancreatic duct (PD) guidewire placement for DGW is sometimes impossible. Endoscopic ultrasound (EUS)-guided biliary access, such as the rendezvous technique, is increasingly used when cannulation has failed but this technique also needs a dilated biliary duct for EUS-guided puncture. We present a successful DGW biliary cannulation using PD guidewire placement under EUS guidance [5] in a patient with failed biliary access by ERCP and EUS ([Video 1]).
Ensemble learning predicts multiple sclerosis disease course in the SUMMIT study
Authors: Yijun Zhao, Tong Wang, Riley Bove, Bruce Cree, Roland Henry, Hrishikesh Lokhande, Mariann Polgar-Turcsanyi, Mark Anderson, Rohit Bakshi, Howard L. Weiner, Tanuja Chitnis
Journal: npj Digital Medicine (2020)

The rate of disability accumulation varies across multiple sclerosis (MS) patients. Machine learning techniques may offer more powerful means to predict disease course in MS patients. In our study, 724 patients from the Comprehensive Longitudinal Investigation in MS at Brigham and Women's Hospital (CLIMB study) and 400 patients from the EPIC dataset, University of California, San Francisco, were included in the analysis. The primary outcome was an increase in Expanded Disability Status Scale (EDSS) ≥ 1.5 (worsening) or not (non-worsening) at up to 5 years after the baseline visit. Classification models were built using the CLIMB dataset with patients' clinical and MRI longitudinal observations in first 2 years, and further validated using the EPIC dataset. We compared the performance of three popular machine learning algorithms (SVM, Logistic Regression, and Random Forest) and three ensemble learning approaches (XGBoost, LightGBM, and a Meta-learner L). A "threshold" was established to trade-off the performance between the two classes. Predictive features were identified and compared among different models. Machine learning models achieved 0.79 and 0.83 AUC scores for the CLIMB and EPIC datasets, respectively, shortly after disease onset. Ensemble learning methods were more effective and robust compared to standalone algorithms. Two ensemble models, XGBoost and LightGBM were superior to the other four models evaluated in our study. Of variables evaluated, EDSS, Pyramidal Function, and Ambulatory Index were the top common predictors in forecasting the MS disease course. Machine learning techniques, in particular ensemble methods offer increased accuracy for the prediction of MS disease course.
TCT CONNECT-31 Predictive Value of Apoptotic Factor M30 for Negative Left Ventricular Remodeling in Patients Undergoing Primary Angioplasty for ST-Segment Elevation Myocardial Infarction
Authors: Jamal Yusuf, Pranav Vaidya, Ankur Gautam, Saibal Mukhopadhyay
Journal: Journal of the American College of Cardiology (2020)
TCT CONNECT-304 Meta-Analysis of Long-Term Outcomes in Patients With Diabetes Mellitus and Left Main Coronary Artery Disease Treated With Drug-Eluting Stent Versus Coronary Artery Bypass Grafting
Authors: Abdullah Al-Abcha, Mark Terence Mujer, Manel Boumegouas, Lawrenshey Charles, Adnan Halbouni, Yehia Saleh
Journal: Journal of the American College of Cardiology (2020)
TCT CONNECT-182 Outcomes of Patients With Cardiogenic Shock Requiring Ventricular Assist Devices in Northern Ontario: Quality Improvement Project
Authors: Sami Alnasser, Derek Manchuk, Bhanu Nalla, Hooman Hennessey, Abdulrahman Alqahtani, Tova Jessup, Robert Anderson, Rony Atoui
Journal: Journal of the American College of Cardiology (2020)
Pembrolizumab plus axitinib versus sunitinib monotherapy as first-line treatment of advanced renal cell carcinoma (KEYNOTE-426): extended follow-up from a randomised, open-label, phase 3 trial
Authors: Thomas Powles, Elizabeth R Plimack, Denis Soulières, Tom Waddell, Viktor Stus, Rustem Gafanov, Dmitry Nosov, Frédéric Pouliot, Bohuslav Melichar, Ihor Vynnychenko, Sergio J Azevedo, Delphine Borchiellini, Raymond S McDermott, Jens Bedke, Satoshi Tamada, Lina Yin, Mei Chen, L Rhoda Molife, Michael B Atkins, Brian I Rini
Journal: The Lancet Oncology (2020)
TCT CONNECT-66 Ischemic and Bleeding Outcomes in Patients With Versus Without Atrial Fibrillation: Analysis From the Onyx ONE Month DAPT Program
Authors: Sanjeevan Pasupati, Azeem Latib, Elvin Kedhi, Ajay Kirtane, David Kandzari, Roxana Mehran, Matthew Price, Daniel Simon, Alexandre Abizaid, Stephen G. Worthley, Azfar Zaman, Maria Parke, Te-Hsin Lung, Stephan Windecker, Gregg Stone
Journal: Journal of the American College of Cardiology (2020)
AB0099 Adult onset still’s disease revelaled by pericardial tamponade and myocarditis
Authors: S Aloui, H Ben Fredj, I Bouajina, M Khelifa, F Bahri, MH Abdessalem, T Chekir
Journal: Annals of the Rheumatic Diseases (2001)

<h3>Background</h3> Pericardial tamponade and myocarditis are very seldom complication of adult onset Still’s disease and exceptionally revealed the disease. A18-year- old man was hospitalised for fever, polyartharalgia, dyspnea and thoracic pain. Chest radiograph demonstrated large cardiac sillouette. The electrocardiogram showed tachycardia and non specific diffuse ST-T changes. Echocardiography revealed a mild alteration of left ventricular function and circumferential pericardial effusion with diastolic collapse. A pericardiocentesis yielded 300 ml of sersanguinos fluid. The white blood cell count was 41200 with 96% neutophils. An extensive evaluation for infection was unrevealing and tests for antinuclear factors and rheumtoid factor were negative. Still’s disease was diagnosed. The patient was treated with prednisone 1 mg/kg/day and rapidly improved. <h3>Objectives</h3> <h3>Methods</h3> <h3>Results</h3> <h3>Conclusion</h3>
Association between atopic dermatitis and autoimmune diseases: a population‐based case–control study*
Authors: L.U. Ivert, C.‐F. Wahlgren, B. Lindelöf, H. Dal, M. Bradley, E.K. Johansson
Journal: British Journal of Dermatology (2020)

Atopic dermatitis (AD) is a common chronic skin disorder and is well known to be associated with other atopic conditions. There is increasing evidence for an association also with nonatopic conditions, including autoimmune diseases, but data are limited about several autoimmune diagnoses.To investigate the association between AD and autoimmune diseases.This case-control study used Swedish national healthcare registers. The source population comprised the entire Swedish population aged ≥ 15 years from 1968 to 2016. Cases, including all those with an inpatient diagnosis of AD (from 1968) and/or a specialist outpatient diagnosis of AD (from 2001), were matched by sex and age to healthy controls (104 832 cases of AD, 1 022 435 controls).AD was significantly associated with one or more autoimmune diseases compared with controls - adjusted odds ratio (aOR) 1·97, 95% confidence interval (CI) 1·93-2·01 - and this association was significantly stronger in the presence of multiple autoimmune diseases compared with only one. The association was strongest for autoimmune disorders involving the skin (aOR 3·10, 95% CI 3·02-3·18), the gastrointestinal tract (aOR 1·75, 95% CI 1·69-1·82) or connective tissue (aOR 1·50, 95% CI 1·42-1·58). In the overall analysis, men with AD had a stronger association with rheumatoid arthritis and coeliac disease than did women with AD. In subanalyses, the findings remained stable in multivariable analyses after adjustment for smoking and parental autoimmune disease.This large population-based study indicates significant autoimmune comorbidity of adults with AD, especially between AD and autoimmune dermatological, gastrointestinal and rheumatological diseases. Having multiple autoimmune diseases resulted in a stronger association with AD than having only one autoimmune disease.
Epidermal growth factor-induced activation of the insulin-like growth factor I receptor in rat hepatocytes
Authors: Hazem Hallak, Giesla Moehren, Jei Tang, Mohamad Kaou, Mouhamad Addas, Jan B. Hoek, Raphael Rubin
Journal: Hepatology (2002)

Insulin-like growth factor I (IGF-I) plays a critical role in the induction of cell cycle progression and survival in many cell types. However, there is minimal IGF-I binding to hepatocytes, and a role for IGF-I in hepatocyte signaling has not been elucidated. The dynamics of IGF-I receptor (IGF-IR) activation were examined in freshly isolated rat hepatocytes. IGF-I did not activate the IGF-IR. However, des(1-3)IGF-I, which weakly binds IGF binding protein-3 (IGFBP-3), induced IGF-IR phosphorylation. IGFBP-3 surface coating was identified by confocal immunofluorescence microscopy. In contrast with the inactivity of IGF-I, epidermal growth factor (EGF) induced the tyrosine phosphorylation of the IGF-IR in parallel with EGF receptor phosphorylation. Transactivation of the IGF-IR by EGF was inhibited by tyrphostin I-Ome-AG538, a tyrosine kinase inhibitor with high specificity for the IGF-IR. Src kinase inhibitors pyrazolopyrimidine PP-1 and PP-2 inhibited transactivation of the IGF-IR by EGF. EGF stimulated the tyrosine phosphorylation of Src, and induced its association with the IGF-IR. EGF-induced phosphorylations of insulinrelated substrate (IRS)-1, IRS-2, Akt, and p42/44 mitogen-activated protein kinases (MAPKs) were inhibited variably by I-Ome-AG538. In conclusion, the data show an EGF- and Src-mediated transactivation pathway for IGF-IR activation in hepatocytes, and indicate a role for the IGF-IR in hepatocyte intracellular signaling. The findings also show a role for IGFBP-3 in the inhibition of IGF-I signaling in hepatocytes. (Hepatology2002;36:1509-1518).
3D Coaxial Printing Tough and Elastic Hydrogels for Tissue Engineering Using a Catechol Functionalized Ink System
Authors: Ying Zhou, Zhilian Yue, Zhi Chen, Gordon Wallace
Journal: Advanced Healthcare Materials (2020)

Abstract 3D printing is now popular in tissue engineering as it provides a facile route to the fabrication of scaffolds with/without living cells with a predesigned geometry. The properties of the ink constituents are critical for printing structures to meet both mechanical and biological requirements. Despite recent advances in ink development, it remains a challenge to print biopolymer based tough and elastic hydrogels. These hydrogels are in great demand as they can mimic the biomechanics of soft tissues such as skin, muscle, and cartilage. In this study, a catechol functionalized ink system is developed for 3D coaxial printing tough and elastic hydrogels. The ink is based on biopolymers including catechol modified hyaluronic acid (HACA) and alginate. A novel crosslinking strategy is proposed, involving simple ionic crosslinking, catechol mediated crosslinking, and Michael addition that are all induced under mild conditions. The HACA and alginate form a double network with high fracture toughness and elasticity, while proteins such as gelatin can be integrated with the HACA/alginate hydrogel during printing to improve cell interactions. The printed constructs demonstrate high cytocompatibility and support the differentiation of myoblasts into aligned myotubes. The catechol functionalized ink can be further modified to target various applications in soft tissue engineering.
Polysocial Risk Scores for Assessing Social Determinants of Health—Reply
Authors: Jose F. Figueroa, Ashish K. Jha
Journal: JAMA (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Primary Chronic Lymphocytic Leukemia Cells Can be Maintained Long-Term in Serum-Free, Cytokine-Free 3D Culture
Authors: Joana Dos Santos, Louise Enfield, Susana Brito Dos Santos, Mark C. Allenby, Sophie Zemenides, Athanasios Mantalaris, Nicki Panoskaltsis
Journal: Blood (2017)
The ethics of transferring adult patients to paediatric services
Authors: Thomas Hampton, Victoria Sadlers
Journal: BMJ (2020)

Oliver discusses some ethical issues that arise from changing pathways of care to manage demand.1 We present another altered treatment paradigm from the first wave of covid-19 that required novel ethical decision making. Similar to Massachusetts General Hospital for Children,2 Alder Hey Children’s NHS Foundation Trust accommodated adults in its paediatric intensive care unit. Fortunately, few local children with covid-19 were admitted to hospital, so the trust offered to relieve pressure on adult services.

Anticipating …
Improving Pretreatment Risk Prognostication in Localized Prostate Cancer
Authors: Yaw A. Nyame, Willie Underwood
Journal: JAMA Oncology (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Oncology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Oncology journal