tbuckley/cabot-search huggingface dataset:
PB2279 EFFECT OF EDUCATIONAL TRAINING PROGRAM ON IMPROVING PEDIATRIC ONCOLOGY NURSES, PRACTICE REGARDING NON PHARMACOLOGICAL PAIN MANAGEMENT
Authors: M. Hesham, A. El‐ Dakhakhny, R. Ramadan, M. besheer
Journal: HemaSphere (2019)

Background: Pain in cancer children poses a significant challenge for health professionals. It is most often not adequately treated because of inexperience and unfounded fears of analgesic treatment. Non pharmacological interventions support patients in coping with the disease consequences and in playing an active role in the management of their condition. Nurses are the professionals most responsible for caring for patient's pain and play a critical role in efficient pain management. Aims: to identify the effect of an educational training program on improvement of pediatric oncology nurses’ practice regarding non‐ pharmacological pain management. Methods: A quasi‐experimental study design was conducted on a sample of 23 nurses who were working in in Pediatric Hematology and Oncology unit at Zagazig university children hospitals. Two tools were used in the present study at three phases (pretest, post‐test and follow up after 2 months); first: a questionnaire interview sheet to assess nurses’ knowledge related to pain and non‐ pharmacological pain management, the second: a structured observational checklist for nurses’ practice.Educational training program was developed to train pediatric oncology nurses about non‐pharmacological pain management in children (Massage technique,Reflexology massage,Hotand cold Pack Application,Slow rhythmic breathing). Results: from the present study we revealed that none of the studied nurses had good knowledge score before implementation of the program. This percentage increased to 100.0% after implementation of the program and decreased to 52.2% at follow up phases of the program. Pediatric oncology nurses practice had been improved significantly after implementation of the educational training program either immediately or 2 months later. Summary/Conclusion: it was concluded that educational training program to pediatric oncology nurses had improved their knowledge and practice regarding non pharmacological pain management as there was a statistical significant difference throughout the three phases of the study. Based on the results of the present study continuous formal training programs for updating the knowledge and practice of pediatric oncology nurses about non‐pharmacological pain management was recommended.
A systematic review of existing ageism scales
Authors: Liat Ayalon, Pnina Dolberg, Sarmitė Mikulionienė, Jolanta Perek-Białas, Gražina Rapolienė, Justyna Stypinska, Monika Willińska, Vânia de la Fuente-Núñez
Journal: Ageing Research Reviews (2019)

Ageism has been shown to have a negative impact on older people's health and wellbeing. Though multiple scales are currently being used to measure this increasingly important issue, syntheses of the psychometric properties of these scales are unavailable. This means that existing estimates of ageism prevalence may not be accurate. We conducted a systematic review aimed at identifying available ageism scales and evaluating their scope and psychometric properties. A comprehensive search strategy was used across fourteen different databases, including PubMed and CINAHL. Independent reviewers extracted data and appraised risk of bias following the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) guidelines. Of the 29,664 records identified, 106 studies, assessing 11 explicit scales of ageism, were eligible for inclusion. Only one scale, the 'Expectations Regarding Aging' met minimum requirements for psychometric validation (i.e., adequate content validity, structural validity and internal consistency). Still, this scale only assesses the 'stereotype' dimension of ageism, thus failing to evaluate the other two ageism dimensions (prejudice and discrimination). This paper highlights the need to develop and validate a scale that accounts for the multidimensional nature of ageism. Having a scale that can accurately measure ageism prevalence is key in a time of increasing and rapid population ageing, where the magnitude of this phenomenon may be increasing.
PB1957 EVALUATION OF HEMATOLOGICAL AND NONHEMATOLOGICAL COMPLICATIONS OF IMATINIB MESILATE (IM) IN PATIENTS WITH PHILADELPHIA POSITIVE CHRONIC MYELOGENOUS LEUKEMIA (PH+ CML)
Authors: A. Ter‐Grigoryan, N. Melkikyan, S. Danelyan
Journal: HemaSphere (2019)

Background: Chronic myelogenous leukemia (CML) is caused by the BCR‐ABL tyrosine kinase, the product of Philadelphia chromosome. Imatinib mesylate (IM) is a selective inhibitor of this kinase. Aims: The objective of this study was to determine the hematological and non‐hematological complications of IM in patient with CML in Armenian population. Methods: The object of the study were patients of the Hematology Center of the Ministry of Health of the Republic of Armenia, registered from 2000 to 2017. During this period 377 patients with a diagnosis of Ph+ CML were observed. Total of 326 (86%) patients treated with IM were involved in research (45.4% male and 54.6% female, who were at the age of 7‐81 years). The 282 patients (86.5%) were in chronic phase, 44 patients (13.5%) ‐ in phase of acceleration and blast crisis. The doses of IM were 400 mg daily for the chronic phase and 600‐800 mg daily for the accelerated and blast phase of disease. The patients were thoroughly monitored for both hematological and non‐hematological complications, in some cases temporary dose adjustment was necessary. Results: 213 patients experienced various side effects which, in decreasing order of frequency were: peripheral edema, bone pains, joint pains, allergic reactions, muscle cramps, nausea, vomiting, hepatic enzyme elevation, gastric pain and headaches as non‐hematologic complications (table 1) and leucopenia, thrombocytopenia and anemia as hematological complications (table 2).Three patients developed grade IV complications in form of toxic hepatitis, anasarca and allergic reaction, which required treatment discontinuation. Cytopenias developed in cases of 48 patients, and CHR/complete hematological response/ within the first 4 months from the start of therapy was observed in 65.3% of their cases. At the same time, in the group of patients who did not have these complications, CHR was achieved in 84% of cases (p = 0.0207). It is noteworthy that in case of patients with cytopenias, CCR/complete cytogenetic response/ was achieved in 15.3% of cases, whereas in case of patients without these complications‐ in 32.4% of cases (p = 0.0754). Thus, hematological complications in the form of cytopenias, which require a temporary cessation of treatment, reduce the likelihood of CHR and CCR. Such statistics show that hematological complications, quite possibly, were originally due to patients belonging to a higher prognostic group. And the group of patients, who in the first 4 months did not reach the CHR, requires a broader cytogenetic examination according to the karyotype and, if necessary, sequencing. Summary/Conclusion: Imatinib mesylate is a well‐tolerated drug with some adverse events that rarely require treatment discontinuation. image
Rbfox1 Mediates Cell-type-Specific Splicing in Cortical Interneurons
Authors: Brie Wamsley, Xavier Hubert Jaglin, Emilia Favuzzi, Giulia Quattrocolo, Maximiliano José Nigro, Nusrath Yusuf, Alireza Khodadadi-Jamayran, Bernardo Rudy, Gord Fishell
Journal: Neuron (2018)
Abstract 15111: Ctrp9 Enhances the Proliferation, Survival, and Paracrine Effect of Engrafted Adipose-Derived Mesenchymal Stem Cells (adsc) in Post-mi Mouse Heart
Authors: Wenjun Yan, Yongzhen Guo, Gan Lu, Yue Chen, Wayne Lau, Ling Tao, Erhe Gao, Walter Koch, Yajing Wang, Xin Ma
Journal: Circulation (2016)

Objective: The poor survival of engrafted stem cells in the ischemic environment limits their therapeutic efficacy for myocardial infarction (MI) treatment. Previously, we demonstrated that adminis...
PB1866 SUCCESSFUL MANAGEMENT OF A CHILD FOR PAROXYSMAL NOCTURNAL HEMOGLOBINURIA WITH LONG‐TERM ECULIZUMAB THERAPY
Authors: G. Aydogan, Z. Salcioglu, F. Akici, E. Yilmaz, T.N. Tahtakesen, N. Bayram, N. Ozdemir
Journal: HemaSphere (2019)

Background: PNH arises from a genetic mutation of hematopoietic stem cells which leads to the acquired nonmalignant clonal expansion of cells lacking glycosyl phosphatidylinositol‐anchored proteins (GPI‐APs). Lack of GPI‐APs translates into PNH's most significant clinical features: bone marrow failure, intravascular hemolysis and thrombosis. PNH rarely occurs in children and has been reported to have a distinct clinical presentation compared to the adult population. Most of the pediatric patients in the litearture have arisen in a previous setting of bone marrow failure. There are few pediatric case reports presented with reccurrent hemolytic episodes. Aims: There is a paucity of published data on outcomes in children with PNH treated with eculizumab. Therefore, we find it significant to share our experience regarding the use of eculizumab for a pediatric patient, who was diagnosed as PNH at 5.5 years of age and have been managed successful by eculizumab for more than 9 years at Kanuni Sultan Süleyman Education and Research Hospital. Methods: The case is retrospectively evaluated. Results: The patient was diagnosed in March 2008 with immunphenotyping and verified with FLAER method. She had been given blood transfusions, hyperhydration therapy, low‐dose oral prednisolone continuously initially and then during hemolytic attacks. During follow‐up, attacks had become more frequent and recurred at 2‐3 weeks intervals. With the informed consent of the family, eculizumab therapy was commenced in December 2011. She was 9 year old at this time.The dose of eculizumab was set according to the weight of the child as 600 mg/week for 4 week, then 900 mg/every other week parenterally. After introduction of eculizumab, both the frequency and the severity of the attacks had declined significantly. She has been under eculizumab therapy for more than 9 years without any considerable advers effect. Reoccurence of hemolytic attack was observed, when the patient had infections or the treatment was interrupted due to problems in obtining drugs. Summary/Conclusion: Depending on our experience, eculizumab may have a substantial role in controlling hemolytic episodes and preventing thrombotic complications and improving quality of life in children similiar to adults.
Abstract 14: Polyethylene Glycol-20k Improves Post-Resuscitation Cerebral Microcirculation in a Rat Model of Cardiac Arrest
Authors: Qinyue Guo, Jin Yang, Zhangle Hu, Yan Xiao, Xiaobo Wu, Jennifer Bradley, Mary Ann Peberdy, Joseph Ornato, Martin Mangino, Wanchun Tang
Journal: Circulation (2018)

Introduction: Polyethylene glycol-20k (PEG-20k) reduces severity of post CPR cerebral dysfunction, however the mechanism is yet unproven. In other vascular beds, PEG-20k osmotically reverses ischemia-induced metabolic cell swelling and decompresses the microcirculation, which improves flow and oxygen transfer. Hypothesis: We hypothesized that PEG-20k improves neurologic function by preserving microcirculatory blood flow and decreasing brain swelling. Methods: 32 S-D rats weighting between 450-550g were randomized into 4 groups: 1) Control (CPR+SALINE); 2) Treated (CPR+PEG-20k); 3) NO CPR+SALINE; 4) NO CPR+PEG-20k. CPR was initiated after 8 min of untreated ventricular fibrillation and continued for 8 min, then defibrillation was performed. PEG-20k was administered at the beginning of chest compression. Cerebral microcirculation was visualized at baseline, 2, 4 and 6 h after CPR by side-stream dark-field (SDF) imaging with a 5х imaging objective. Animals were euthanized with Euthasol (150 mg/kg) and brain swelling was assessed by comparing wet-to-dry weight ratios at 6h post resuscitation. Results: The wet to dry ratio in the CPR+SALINE group was significantly higher than the other three groups (p&lt;0.05). PEG-20k attenuated the wet to dry ratio (p&lt;0.05) compared with the CPR+SALINE group (Fig 1). A significant improvement of cerebral microcirculation was also observed in the CPR+PEG-20k group when compared with the CPR+SALINE group (Fig 2). Conclusions: PEG-20k given during resuscitation improves cerebral microcirculatory flow by increasing microcirculation and decreasing brain tissue swelling in a rat model of CA.
AB0317 THE EFFECT OF ANEMIA ON THE CARDIAC FUNCTION IN PATIENTS WITH RHEUMATOID ARTHRITIS
Authors: Liutsiia Feiskhanova, Svetlana Lapshina, Albina Yusupova, Aigul Akhmetzianova
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Rheumatoid arthritis (RA) is a chronic disease that is characterized by defeat of the musculoskeletal system and is often accompanied by the anemia. <h3>Objectives</h3> The aim of our study was to determine the effect of anemia on the structural and functional state of the heart in patients with rheumatoid arthritis. <h3>Methods</h3> The 154 patients with rheumatoid arthritis were included in the study: 37 patients with anemia (1<sup>st</sup> group) and 117 – without systemic manifestations of rheumatoid arthritis (2<sup>nd</sup> group). RA activity was estimated by index DAS-28. All patients underwent echocardiography. <h3>Results</h3> Both groups were comparable in age, sex, ratio of seropositive and seronegative patients. A comparative analysis of both groups revealed that in 1<sup>st</sup> group DAS-28 was higher than in 2<sup>nd</sup> group (p&lt;0.05). According to the results of echocardiography, the left ventricular myocardial mass index was higher in 1<sup>st</sup> group than in the 2<sup>nd</sup> group (p&lt;0.05). The same applies to the end-diastolic size of the left ventricle: in patients with anemia, it was more than in patients without it (p&lt;0.05). Our results suggest that patients with anemia often have a violation of diastolic myocardial function, which is accompanied by an increase in myocardial mass. The correlation analysis in both groups revealed a direct relationship between the age and the left ventricular mass index (r=0.62, p&lt;0.0005), between the age and the mitral E/a (r=-0.55, p&lt;0.00001). In addition, in 1<sup>st</sup> group we revealed the inverse relationship between the DAS-28 and the tricuspid E/a (r=-0.43, p&lt;0.05). <h3>Conclusion</h3> Thus, regardless of the presence of anemia, the patients with rheumatoid arthritis observed dependence of structural and functional changes in the heart of age: a violation of diastolic function of the left ventricle, an increase of the left ventricular myocardial mass index,. However, in patients with anemia, an increase in the activity of the disease is accompanied by diastolic dysfunction of the right ventricle that requires correction in the tactics of patient management. <h3>References</h3> [1] rheumatoid arthritis, anemia, echocardiography <h3>Disclosure of Interests</h3> None declared
BUSCO Applications from Quality Assessments to Gene Prediction and Phylogenomics
Authors: Robert M Waterhouse, Mathieu Seppey, Felipe A Simão, Mosè Manni, Panagiotis Ioannidis, Guennadi Klioutchnikov, Evgenia V Kriventseva, Evgeny M Zdobnov
Journal: Molecular Biology and Evolution (2017)

Genomics promises comprehensive surveying of genomes and metagenomes, but rapidly changing technologies and expanding data volumes make evaluation of completeness a challenging task. Technical sequencing quality metrics can be complemented by quantifying completeness of genomic data sets in terms of the expected gene content of Benchmarking Universal Single-Copy Orthologs (BUSCO, http://busco.ezlab.org). The latest software release implements a complete refactoring of the code to make it more flexible and extendable to facilitate high-throughput assessments. The original six lineage assessment data sets have been updated with improved species sampling, 34 new subsets have been built for vertebrates, arthropods, fungi, and prokaryotes that greatly enhance resolution, and data sets are now also available for nematodes, protists, and plants. Here, we present BUSCO v3 with example analyses that highlight the wide-ranging utility of BUSCO assessments, which extend beyond quality control of genomics data sets to applications in comparative genomics analyses, gene predictor training, metagenomics, and phylogenomics.
<i>HHIPL1</i> , a Gene at the 14q32 Coronary Artery Disease Locus, Positively Regulates Hedgehog Signaling and Promotes Atherosclerosis
Authors: Dimitra Aravani, Gavin E. Morris, Peter D. Jones, Helena K. Tattersall, Elisavet Karamanavi, Michael A. Kaiser, Renata B. Kostogrys, Maryam Ghaderi Najafabadi, Sarah L. Andrews, Mintu Nath, Shu Ye, Emma J. Stringer, Nilesh J. Samani, Tom R. Webb
Journal: Circulation (2019)

Genome-wide association studies have identified chromosome 14q32 as a locus for coronary artery disease. The disease-associated variants fall in a hitherto uncharacterized gene called HHIPL1 (hedgehog interacting protein-like 1), which encodes a sequence homolog of an antagonist of hedgehog signaling. The function of HHIPL1 and its role in atherosclerosis are unknown.HHIPL1 cellular localization, interaction with sonic hedgehog (SHH), and influence on hedgehog signaling were tested. HHIPL1 expression was measured in coronary artery disease-relevant human cells, and protein localization was assessed in wild-type and Apoe-/- (apolipoprotein E deficient) mice. Human aortic smooth muscle cell phenotypes and hedgehog signaling were investigated after gene knockdown. Hhipl1-/- mice were generated and aortic smooth muscle cells collected for phenotypic analysis and assessment of hedgehog signaling activity. Hhipl1-/- mice were bred onto both the Apoe-/- and Ldlr-/- (low-density lipoprotein receptor deficient) knockout strains, and the extent of atherosclerosis was quantified after 12 weeks of high-fat diet. Cellular composition and collagen content of aortic plaques were assessed by immunohistochemistry.In vitro analyses revealed that HHIPL1 is a secreted protein that interacts with SHH and increases hedgehog signaling activity. HHIPL1 expression was detected in human smooth muscle cells and in smooth muscle within atherosclerotic plaques of Apoe-/- mice. The expression of Hhipl1 increased with disease progression in aortic roots of Apoe-/- mice. Proliferation and migration were reduced in Hhipl1 knockout mouse and HHIPL1 knockdown aortic smooth muscle cells, and hedgehog signaling was decreased in HHIPL1-deficient cells. Hhipl1 knockout caused a reduction of >50% in atherosclerosis burden on both Apoe-/- and Ldlr-/- knockout backgrounds, and lesions were characterized by reduced smooth muscle cell content.HHIPL1 is a secreted proatherogenic protein that enhances hedgehog signaling and regulates smooth muscle cell proliferation and migration. Inhibition of HHIPL1 protein function might offer a novel therapeutic strategy for coronary artery disease.
Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
Authors: Alexis Vandenbon, Yutaro Kumagai, Mengjie Lin, Yutaka Suzuki, Kenta Nakai
Journal: Genome biology (2018)

The importance of transcription factors (TFs) and epigenetic modifications in the control of gene expression is widely accepted. However, causal relationships between changes in TF binding, histone modifications, and gene expression during the response to extracellular stimuli are not well understood. Here, we analyze the ordering of these events on a genome-wide scale in dendritic cells in response to lipopolysaccharide (LPS) stimulation.Using a ChIP-seq time series dataset, we find that the LPS-induced accumulation of different histone modifications follows clearly distinct patterns. Increases in H3K4me3 appear to coincide with transcriptional activation. In contrast, H3K9K14ac accumulates early after stimulation, and H3K36me3 at later time points. Integrative analysis with TF binding data reveals potential links between TF activation and dynamics in histone modifications. Especially, LPS-induced increases in H3K9K14ac and H3K4me3 are associated with binding by STAT1/2 and were severely impaired in Stat1-/- cells.While the timing of short-term changes of some histone modifications coincides with changes in transcriptional activity, this is not the case for others. In the latter case, dynamics in modifications more likely reflect strict regulation by stimulus-induced TFs and their interactions with chromatin modifiers.
Genome-wide association study implicates CHRNA2 in cannabis use disorder
Authors: Ditte Demontis, Veera Manikandan Rajagopal, Thorgeir E. Thorgeirsson, Thomas D. Als, Jakob Grove, Kalle Leppälä, Daniel F. Gudbjartsson, Jonatan Pallesen, Carsten Hjorthøj, Gunnar W. Reginsson, Thorarinn Tyrfingsson, Valgerdur Runarsdottir, Per Qvist, Jane Hvarregaard Christensen, Jonas Bybjerg-Grauholm, Marie Bækvad-Hansen, Laura M. Huckins, Eli A. Stahl, Allan Timmermann, Esben Agerbo, David M. Hougaard, Thomas Werge, Ole Mors, Preben Bo Mortensen, Merete Nordentoft, Mark J. Daly, Hreinn Stefansson, Kari Stefansson, Mette Nyegaard, Anders D. Børglum
Journal: Nature Neuroscience (2019)
Reforming public hospital financing in China: progress and challenges
Authors: Jin Xu, Weiyan Jian, Kun Zhu, Soonman Kwon, Hai Fang
Journal: BMJ (2019)

Reforming public hospital financing in China: progress and challengesJin Xu and colleagues describe the effects of the financing reforms of public hospitals and suggests steps to further progress towards equitable, efficient, and good quality care.* Major areas targeted by the reform on public hospital financing Drug mark-up removal