tbuckley/cabot-search huggingface dataset:
Abstract 15670: Relationship Between Baseline Cardiac Biomarkers and Cardiovascular Death or Hospitalization for Heart Failure in DECLARE-TIMI 58
Authors: Thomas A Zelniker, David A Morrow, Ofri Mosenzon, Erica Goodrich, Sabina A Murphy, Deepak L Bhatt, Lawrence A Leiter, Darren K McGuire, John P Wilding, Ingrid A Gause-Nilsson, Anna Maria Langkilde, Itamar Raz, Marc S Sabatine, Stephen D Wiviott
Journal: Circulation (2019)

Introduction: Dapagliflozin reduced the risk of the composite of cardiovascular death or hospitalization for heart failure (CVD/HHF) in patients (pts) with type 2 diabetes (T2D) in DECLARE-TIMI 58....
Chronic Myelogenous Leukemia
Authors: Susan O'Brien, Ellin Berman, Hossein Borghaei, Daniel J. DeAngelo, Marcel P. Devetten, Steven Devine, Harry P. Erba, Jason Gotlib, Madan Jagasia, Joseph O. Moore, Tariq Mughal, Javier Pinilla-Ibarz, Jerald P. Radich, Neil P. Shah, Paul J. Shami, B. Douglas Smith, David S. Snyder, Martin S. Tallman, Moshe Talpaz, Meir Wetzler
Journal: Journal of the National Comprehensive Cancer Network (2009)

OverviewChronic myelogenous leukemia (CML) accounts for 15% of adult leukemias.Although the median age of disease onset is 67 years, CML occurs in all age groups (Surveillance, Epidemiology, and End Results [SEER] statistics).In 2009, an estimated 5050 cases will be diagnosed and 470 patients will die from the disease in the United States. 1 CML is a hematopoietic stem cell disease, which is characterized by a reciprocal translocation between chromosomes 9 and 22, resulting in the formation of the Philadelphia chromosome (Ph chromosome).This translocation t(9;22) results in the head-to-tail fusion of the breakpoint cluster region (BCR) gene on chromosome 22 at band q11 and the Abelson murine leukemia (ABL) gene located
PD-L1 targeting high-affinity NK (t-haNK) cells induce direct antitumor effects and target suppressive MDSC populations
Authors: Kellsye P Fabian, Michelle R Padget, Renee N. Donahue, Kristen Solocinski, Yvette Robbins, Clint T. Allen, John H. Lee, Shahrooz Rabizadeh, Patrick Soon-Shiong, Jeffrey Schlom, James W Hodge
Journal: Journal for ImmunoTherapy of Cancer (2020)

Background Although immune checkpoint inhibitors have revolutionized cancer treatment, clinical benefit with this class of agents has been limited to a subset of patients. Hence, more effective means to target tumor cells that express immune checkpoint molecules should be developed. For the first time, we report a novel natural killer (NK) cell line, programmed death-ligand 1 (PD-L1) targeting high-affinity natural killer (t-haNK), which was derived from NK-92 and was engineered to express high-affinity CD16, endoplasmic reticulum-retained interleukin (IL)-2, and a PD-L1-specific chimeric antigen receptor (CAR). We show that PD-L1 t-haNK cells also retained the expression of native NK receptors and carried a high content of granzyme and perforin granules. Methods NanoString, flow cytometry, and immunofluorescence analyses were performed to characterize the phenotype of irradiated PD-L1 t-haNK cells. In vitro PD-L1 t-haNK cell activity against cancer cell lines and human peripheral blood mononuclear cells (PBMCs) was determined via flow-based and 111 In-release killing assays. The antitumor effect of PD-L1 t-haNK cells in vivo was investigated using MDA-MB-231, H460, and HTB1 xenograft models in NOD-scid IL2Rgamma null (NSG) mice. Additionally, the antitumor effect of PD-L1 t-haNK cells, in combination with anti-PD-1 and N-803, an IL-15 superagonist, was evaluated using mouse oral cancer 1 syngeneic model in C57BL/6 mice. Results We show that PD-L1 t-haNK cells expressed PD-L1-targeting CAR and CD16, retained the expression of native NK receptors, and carried a high content of granzyme and perforin granules. In vitro, we demonstrate the ability of irradiated PD-L1 t-haNK cells to lyse 20 of the 20 human cancer cell lines tested, including triple negative breast cancer (TNBC) and lung, urogenital, and gastric cancer cells. The cytotoxicity of PD-L1 t-haNK cells was correlated to the PD-L1 expression of the tumor targets and can be improved by pretreating the targets with interferon (IFN)-γ. In vivo, irradiated PD-L1 t-haNK cells inhibited the growth of engrafted TNBC and lung and bladder tumors in NSG mice. The combination of PD-L1 t-haNK cells with N-803 and anti-PD-1 antibody resulted in superior tumor growth control of engrafted oral cavity squamous carcinoma tumors in C57BL/6 mice. In addition, when cocultured with human PBMCs, PD-L1 t-haNK cells preferentially lysed the myeloid-derived suppressor cell population but not other immune cell types. Conclusion These studies demonstrate the antitumor efficacy of PD-L1 t-haNK cells and provide a rationale for the potential use of these cells in clinical studies.
Effect of structural stability on endolysosomal degradation and T‐cell reactivity of major shrimp allergen tropomyosin
Authors: Sandip D. Kamath, Sandra Scheiblhofer, Christopher M. Johnson, Yoan Machado, Thomas McLean, Aya C. Taki, Paul A. Ramsland, Swati Iyer, Isabella Joubert, Heidi Hofer, Michael Wallner, Josef Thalhamer, Jennifer Rolland, Robyn O’Hehir, Peter Briza, Fatima Ferreira, Richard Weiss, Andreas L. Lopata
Journal: Allergy (2020)

Abstract Background Tropomyosins are highly conserved proteins, an attribute that forms the molecular basis for their IgE antibody cross‐reactivity. Despite sequence similarities, their allergenicity varies greatly between ingested and inhaled invertebrate sources. In this study, we investigated the relationship between the structural stability of different tropomyosins, their endolysosomal degradation patterns, and T‐cell reactivity. Methods We investigated the differences between four tropomyosins—the major shrimp allergen Pen m 1 and the minor allergens Der p 10 (dust mite), Bla g 7 (cockroach), and Ani s 3 (fish parasite)—in terms of IgE binding, structural stability, endolysosomal degradation and subsequent peptide generation, and T‐cell cross‐reactivity in a BALB/c murine model. Results Tropomyosins displayed different melting temperatures, which did not correlate with amino acid sequence similarities. Endolysosomal degradation experiments demonstrated differential proteolytic digestion, as a function of thermal stability, generating different peptide repertoires. Pen m 1 (T m 42°C) and Der p 10 (T m 44°C) elicited similar patterns of endolysosomal degradation, but not Bla g 7 (T m 63°C) or Ani s 3 (T m 33°C). Pen m 1–specific T‐cell clones, with specificity for regions highly conserved in all four tropomyosins, proliferated weakly to Der p 10, but did not proliferate to Bla g 7 and Ani s 3, indicating lack of T‐cell epitope cross‐reactivity. Conclusions Tropomyosin T‐cell cross‐reactivity, unlike IgE cross‐reactivity, is dependent on structural stability rather than amino acid sequence similarity. These findings contribute to our understanding of cross‐sensitization among different invertebrates and design of suitable T‐cell peptide‐based immunotherapies for shrimp and related allergies.
Health Literacy and Outcomes Among Patients With Heart Failure
Authors: Matteo Fabbri, M. Hassan Murad, Alexandra M. Wennberg, Pierpaolo Turcano, Patricia J. Erwin, Fares Alahdab, Alvise Berti, Sheila M. Manemann, Kathleen J. Yost, Lila J. Finney Rutten, Véronique L. Roger
Journal: JACC Heart Failure (2020)
Factors Associated With Chemotherapy-Induced Vomiting Control in Pediatric Patients Receiving Moderately or Highly Emetogenic Chemotherapy: A Pooled Analysis
Authors: L. Lee Dupuis, George A. Tomlinson, Annpey Pong, Lillian Sung, Kara Bickham
Journal: Journal of Clinical Oncology (2020)

To identify factors associated with chemotherapy-induced vomiting (CIV) control in pediatric patients receiving highly emetogenic chemotherapy (HEC) or moderately emetogenic chemotherapy (MEC).An individual, patient-level, pooled analysis was performed using data from five clinical trials of aprepitant or fosaprepitant in pediatric patients receiving HEC or MEC. The proportion of individuals who experienced no vomiting (complete CIV control) during the phase of interest was the primary study end point. The association of acute-phase complete CIV control (from first chemotherapy dose to 24 hours after the last chemotherapy dose of the chemotherapy block) with age, sex, race, cancer type, acute-phase duration, and antiemetic regimen was examined. Association of the same factors and acute-phase complete CIV control with complete CIV control in the delayed phase (end of acute phase until ≤ 96 hours later) was examined.A total of 735 patients (mean age, 8.9 years; range, 0.3 to 17.9 years) were included in the acute-phase analysis. Acute-phase complete CIV control was less likely in older patients (relative risk [RR], 0.97 per year; 95% CI, 0.96 to 0.98 per year) and longer acute-phase duration (RR, 0.89 per day; 95% CI, 0.84 to 0.94 per day). Receipt of ondansetron plus aprepitant or fosaprepitant was associated with a higher likelihood of acute-phase complete CIV control versus ondansetron alone (RR, 1.28; 95% CI, 1.09 to 1.50). Delayed-phase complete CIV control was more likely in patients with acute-phase complete CIV control (RR, 1.19; 95% CI, 1.06 to 1.34) and in those who received aprepitant or fosaprepitant.Younger age, shorter acute-phase duration, and antiemetic regimen were associated with acute-phase complete CIV control in pediatric patients receiving HEC or MEC. Acute-phase complete CIV control and antiemetic regimen were associated with delayed-phase complete CIV control.
1O Neratinib + capecitabine vs lapatinib + capecitabine in HER2+ metastatic breast cancer previously treated with ≥2 HER2-directed regimens: Exploratory biomarker analyses from phase III NALA trial
Authors: C. Saura, A. Vivancos, J. Matito, H. Wildiers, A.M. Brufsky, M. Oliveira, S. Waters, S.A. Hurvitz, B. Moy, S-B. Kim, W.J. Gradishar, G.S. Queiroz, E. Cronemberger, J. Bebchuk, K. Keyvanjah, A.S. Lalani, L.D. Eli, S. Delaloge
Journal: Annals of Oncology (2020)
Computed tomography and photoacoustic imaging guided photodynamic therapy against breast cancer based on mesoporous platinum with insitu oxygen generation ability
Authors: Lingyan Zhang, Mifang Li, Quan Zhou, Meng Dang, Yuxia Tang, Shouju Wang, Jijun Fu, Zhaogang Teng, Guangming Lu
Journal: Acta Pharmaceutica Sinica B (2020)

Photodynamic therapy (PDT) has been widely used in cancer treatment. However, hypoxia in most solid tumors seriously restricts the efficacy of PDT. To improve the hypoxic microenvironment, we designed a novel mesoporous platinum (mPt) nanoplatform to catalyze hydrogen peroxide (H2O2) within the tumor cells in situ without an extra enzyme. During the fabrication, the carboxy terminus of the photosensitizer chlorin e6 (Ce6) was connected to the amino terminus of the bifunctional mercaptoaminopolyglycol (SH-PEG-NH2) by a condensation reaction, and then PEG-Ce6 was modified onto the mPt moiety via the mercapto terminal of SH-PEG-NH2. Material, cellular and animal experiments demonstrated that Pt@PEG-Ce6 catalyzed H2O2 to produce oxygen (O2) and that Ce6 transformed O2 to generate reactive oxygen species (ROS) upon laser irradiation. The Pt@PEG-Ce6 nanoplatform with uniform diameter presented good biocompatibility and efficient tumor accumulation. Due to the high atomic number and good near-infrared absorption for Pt, this Pt@PEG-Ce6 nanoplatform showed computed tomography (CT) and photoacoustic (PA) dual-mode imaging ability, thus providing an important tool for monitoring the tumor hypoxic microenvironment. Moreover, the Pt@PEG-Ce6 nanoplatform reduced the expression of hypoxia-inducible factor-1α (HIF-1α) and programmed death-1 (PD-1) in tumors, discussing the relationship between hypoxia, PD-1, and PDT for the first time.
166P Awareness and availability of routine germline BRCA1/2 (gBRCA1/2) mutation testing in patients (pts) with advanced breast cancer (ABC) in Germany
Authors: M.P. Lux, T. Decker, E.D. Runkel, A. Niyazov, R.G.W. Quek, E. Glastetter, N.W. Marschner, N. Harbeck
Journal: Annals of Oncology (2020)
A Combined Morphometric and Statistical Approach to Assess Nonmonotonicity in the Developing Mammary Gland of Rats in the CLARITY-BPA Study
Authors: Maël Montévil, Nicole Acevedo, Cheryl M. Schaeberle, Manushree Bharadwaj, Suzanne E. Fenton, Ana M. Soto
Journal: Environmental Health Perspectives (2020)

The Consortium Linking Academic and Regulatory Insights on Bisphenol-A (CLARITY-BPA) is a rare collaboration of guideline-compliant (core) studies and academic hypothesis-based studies to assess the effects of bisphenol A (BPA).We aimed to a) determine whether BPA showed effects on the developing rat mammary gland using new quantitative and established semiquantitative methods in two laboratories, b) develop a software tool for automatic evaluation of quantifiable aspects of the mammary ductal tree, and c) compare those methods.Sprague-Dawley rats were exposed to BPA, vehicle, or positive control [ethinyl estradiol (EE2)] by oral gavage beginning on gestational day (GD)6 and continuing with direct dosing of the pups after birth. There were two studies: subchronic and chronic. The latter used two exposure regimes, one stopping at postnatal day (PND)21 (stop-dose) the other continuing until tissue harvest (continuous). Glands were harvested at multiple time points; whole mounts and histological specimens were analyzed blinded to treatment.The subchronic study's semiquantitative analysis revealed no significant differences between control and BPA dose groups at PND21, whereas at PND90 there were significant differences between control and the lowest BPA dose and between control and the lowest EE2 dose in animals in estrus. Quantitative, automatized analysis of the chronic PND21 specimens displayed nonmonotonic BPA effects, with a breaking point between the 25 and 250μg/kg body weight (BW) per day doses. This breaking point was confirmed by a global statistical analysis of chronic study animals at PND90 and 6 months analyzed by the quantitative method. The BPA response was different from the EE2 effect for many features.Both the semiquantitative and the quantitative methods revealed nonmonotonic effects of BPA. The quantitative unsupervised analysis used 91 measurements and produced the most striking nonmonotonic dose-response curves. At all time points, lower doses resulted in larger effects, consistent with the core study, which revealed a significant increase of mammary adenocarcinoma incidence in the stop-dose animals at the lowest BPA dose tested. https://doi.org/10.1289/EHP6301.
Abstract 14930: Salt-Sensitive Hypertension is Blunted by Deletion of G Protein-Coupled Receptor 35 Gene in Mice
Authors: Hainan Li, Megan M O’Meara, Sai Pranathi Meda Venkata, Jie-mei Wang
Journal: Circulation (2019)

Introduction: G protein-coupled receptor 35 (GPR35) is a poorly characterized receptor with controversial endogenous ligands and unclear intracellular signaling pathways. Links between GPR35 and hy...
Peyronie Disease as an Example of Nononcological, Inflammatory Uptake of 18F-Fluorocholine in Patient With Prostate Cancer
Authors: Paulina Cegla, Anna Kubiak, Kamila Witkowska, Rafał Czepczyński
Journal: Clinical Nuclear Medicine (2020)

Abstract 18 F-fluorocholine PET/CT is commonly used for staging and assessing treatment response in prostate cancer patients. Growing clinical experience has shown that 18 F-fluorocholine can actually accumulate in sites of inflammation. We report a rare case of a prostate cancer patient with incidentally detected Peyronie disease.
만성 바이러스성 간질환 환자의 삶의 질 평가 및 관련인자 분석
Authors: 박창근, 박수영, 김은수, 박진형, 현동우, 윤영미, 조창민, Won Young Tak, 권영오, Sung Kook Kim, Yong-Hwan Choi, 박신구
Journal: Clinical and Molecular Hepatology (2003)

목적: 최근 질병의 자연경과나 치료에 따른 건강 상태의 변화를 평가하는데 있어 건강 관련 삶의 질 측정이 중요하게 인식되고 있다. 우리나라에서 주요한 만성 질환의 하나인 만성 바이러스성 간염은 아직 근본적 치료가 제한되어 있고, 궁극적으로 간경변이나 간암으로 진행할 수 있다는 점에서 질병 초기부터 환자의 삶의 질에 영향을 미칠 것으로 본다. 이에 저자들은 만성 바이러스성 간질환 환자에서 삶의 질을 평가하고, 이들의 삶의 질에 영향을 미치는 임상 변수를 분석하고자 하였다. 대상과 방법: 본원 소화기내과에서 치료 중인 만성 바이러스성 간염 및 간경변 환자 365명과 건강 검진을 시행한 750명의 대조군을 대상으로 한국어판 SF-36을 이용하여 삶의 질을 측정 및 비교하였으며, 환자군의 인구 통계학적 및 임상 변수와 삶의 질의 각 항목 별 점수의 상관 정도를 분석하였다. 결과: 대조군과 비교하였을 때 환자군에서 SF-36의 대부분의 항목에서 삶의 질이 낮았으며, 특히 이러한 차이는 신체적 건강을 나타내는 영역보다 정신적 건강을 나타내는 영역에서 더 현저하였고, 전반적으로 질병의 중증도가 심할수록 각 항목 별 점수가 낮았다. 또한 정신 건강을 나타내는 영역에서는 간염 및 초기 간경변(Child A)에서부터 삶의 질이 낮았으나, 신체 건강을 나타내는 영역에서는 질병이 상당히 진행하여서야(Child B or C) 삶의 질이 낮아짐을 알 수 있었다. B형 간염과 C형 간염을 비교하였을 때 두 군간의 유의한 차이는 없었다. 조직학적 활성도, AST치, ALT치, 질병의 이환 기간, HBV DNA 수치는 삶의 질과 유의한 상관 관계를 보이지 않았고, 나이, 혈청 알부민, 빌리루빈, 프로트롬빈 시간은 유의한 상관 관계를 보였다. 결론: 만성 바이러스성 간염 및 간경변 환자는 질병의 중증도에 따라 다양한 삶의 질의 저하를 보였으며, 신체적 건강에 비해 정신적 건강을 나타내는 영역에서 질병의 초기부터 삶의 질이 저하됨을 알 수 있다. 따라서 치료 효과나 질병의 진행 정도를 평가하는데 있어, 건강 관련 삶의 질 측정에 더 많은 관심이 필요할 것으로 생각된다.
Chimpanzee ethnography reveals unexpected cultural diversity
Authors: Christophe Boesch, Ammie K. Kalan, Roger Mundry, Mimi Arandjelovic, Simone Pika, Paula Dieguez, Emmanuel Ayuk Ayimisin, Amanda Barciela, Charlotte Coupland, Villard Ebot Egbe, Manasseh Eno-Nku, J. Michael Fay, David Fine, R. Adriana Hernandez-Aguilar, Veerle Hermans, Parag Kadam, Mohamed Kambi, Manuel Llana, Giovanna Maretti, David Morgan, Mizuki Murai, Emily Neil, Sonia Nicholl, Lucy Jayne Ormsby, Robinson Orume, Liliana Pacheco, Alex Piel, Crickette Sanz, Lilah Sciaky, Fiona A. Stewart, Nikki Tagg, Erin G. Wessling, Jacob Willie, Hjalmar S. Kühl
Journal: Nature Human Behaviour (2020)
Prevalence and Clinical Presentation of Health Care Workers With Symptoms of Coronavirus Disease 2019 in 2 Dutch Hospitals During an Early Phase of the Pandemic
Authors: Marjolein F. Q. Kluytmans-van den Bergh, Anton G. M. Buiting, Suzan D. Pas, Robbert G. Bentvelsen, Wouter van den Bijllaardt, Anne J. G. van Oudheusden, Miranda M. L. van Rijen, Jaco J. Verweij, Marion P. G. Koopmans, Jan A. J. W. Kluytmans
Journal: JAMA Network Open (2020)

<h3>Importance</h3> On February 27, 2020, the first patient with coronavirus disease 2019 (COVID-19) was reported in the Netherlands. During the following weeks, at 2 Dutch teaching hospitals, 9 health care workers (HCWs) received a diagnosis of COVID-19, 8 of whom had no history of travel to China or northern Italy, raising the question of whether undetected community circulation was occurring. <h3>Objective</h3> To determine the prevalence and clinical presentation of COVID-19 among HCWs with self-reported fever or respiratory symptoms. <h3>Design, Setting, and Participants</h3> This cross-sectional study was performed in 2 teaching hospitals in the southern part of the Netherlands in March 2020, during the early phase of the COVID-19 pandemic. Health care workers employed in the participating hospitals who experienced fever or respiratory symptoms were asked to voluntarily participate in a screening for infection with the severe acute respiratory syndrome coronavirus 2. Data analysis was performed in March 2020. <h3>Main Outcomes and Measures</h3> The prevalence of severe acute respiratory syndrome coronavirus 2 infection was determined by semiquantitative real-time reverse transcriptase–polymerase chain reaction on oropharyngeal samples. Structured interviews were conducted to document symptoms for all HCWs with confirmed COVID-19. <h3>Results</h3> Of 9705 HCWs employed (1722 male [18%]), 1353 (14%) reported fever or respiratory symptoms and were tested. Of those, 86 HCWs (6%) were infected with severe acute respiratory syndrome coronavirus 2 (median age, 49 years [range, 22-66 years]; 15 [17%] male), representing 1% of all HCWs employed. Most HCWs experienced mild disease, and only 46 (53%) reported fever. Eighty HCWs (93%) met a case definition of fever and/or coughing and/or shortness of breath. Only 3 (3%) of the HCWs identified through the screening had a history of travel to China or northern Italy, and 3 (3%) reported having been exposed to an inpatient with a known diagnosis of COVID-19 before the onset of symptoms. <h3>Conclusions and Relevance</h3> Within 2 weeks after the first Dutch case was detected, a substantial proportion of HCWs with self-reported fever or respiratory symptoms were infected with severe acute respiratory syndrome coronavirus 2, likely as a result of acquisition of the virus in the community during the early phase of local spread. The high prevalence of mild clinical presentations, frequently not including fever, suggests that the currently recommended case definition for suspected COVID-19 should be used less stringently.
Response to: ‘Phsician's global assessment is often useful in SLE, but not always: the case of clinical remission’ by Zenet al
Authors: Anca Askanase, Shereen Oon, Molla Huq, Alicia Calderone, Eric F Morand, Mandana Nikpour, Cynthia Aranow
Journal: Annals of the Rheumatic Diseases (2020)

We thank Dr Zen and colleagues for their interest in our article on the Physician’s Global Assessment (PGA)1 in lupus, and for sharing their experience.2 Their comment focusses on the impact of a PGA (scored prior to reviewing complement and anti-DNA antibody tests) in association with the clinical Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) (cSLEDAI) on the definition of clinical remission. Of note, the cSLEDAI captures haematology and renal …
Abstract 1545: Insulating an Open Irrigated Ablation Electrode Improves Electrode Temperature Feedback for Radiofrequency Lesion Control
Authors: Katsuaki Yokoyama, Hiroshi Nakagawa, Fred H Wittkampf, Atsushi Ikeda, Jeremy D Dando, Huisun Wang, Harry A Puryear, Jan V Pitha, Ralph Lazzara, Warren M Jackman
Journal: Circulation (2007)

Open irrigation (Irrig) cools the electrode and electrode-tissue interface (ETI), preventing thrombus. Improving electrode temperature (ET) feedback by insulating the Irrig electrode internally may help prevent excessive tissue heating. The purpose was to compare an insulated Irrig electrode to a conventional Irrig electrode in ET response and lesion formation using a canine thigh muscle preparation. Methods In 11 dogs, the skin over the thigh muscle was incised and raised to form a cradle which was superfused with heparinized blood at 37°C. The 7F insulated catheter has 6 Irrig holes proximal to a 2.5mm tip electrode (spray around electrode and ETI) and one thermally insulated Irrig channel through the electrode (only external cooling, Ext Cool, St Jude Medical). The Ext Cool and a conventional 7F, 2.5mm open Irrig catheter (external and internal electrode cooling, Ext/Int Cool, St Jude Medical) were held perpendicular or parallel to thigh muscle. RF was applied for 60 sec (n=176) at 10, 15, 20, or 25W with saline Irrig at 17ml/min in low blood flow (&lt;0.1 m/sec) to favor thrombus. ET, ETI and tissue temperatures (3 and 7mm depths) were measured. After each RF, blood was removed to examine for thrombus. Results Table and Fig Conclusions Ext Cool had: greater correlation between ET and both lesion depth (r=0.84 vs 0.54) and steam pop (ET≥51°C vs ET 32– 48°C); lower ETI temperature and less thrombus.