Effect of Genotype-Guided Oral P2Y12 Inhibitor Selection vs Conventional Clopidogrel Therapy on Ischemic Outcomes After Percutaneous Coronary Intervention
Authors: Naveen L. Pereira, Michael E. Farkouh, Derek So, Ryan Lennon, Nancy Geller, Verghese Mathew, Malcolm Bell, Jang-Ho Bae, Myung Ho Jeong, Ivan Chavez, Paul Gordon, J. Dawn Abbott, Charles Cagin, Linnea Baudhuin, Yi-Ping Fu, Shaun G. Goodman, Ahmed Hasan, Erin Iturriaga, Amir Lerman, Mandeep Sidhu, Jean-Francois Tanguay, Liewei Wang, Richard Weinshilboum, Robert Welsh, Yves Rosenberg, Kent Bailey, Charanjit Rihal
Journal: JAMA (2020)

<h3>Importance</h3> After percutaneous coronary intervention (PCI), patients with<i>CYP2C19*2</i>or<i>*3</i>loss-of-function (LOF) variants treated with clopidogrel have increased risk of ischemic events. Whether genotype-guided selection of oral P2Y12 inhibitor therapy improves ischemic outcomes is unknown. <h3>Objective</h3> To determine the effect of a genotype-guided oral P2Y12 inhibitor strategy on ischemic outcomes in<i>CYP2C19</i>LOF carriers after PCI. <h3>Design, Setting, and Participants</h3> Open-label randomized clinical trial of 5302 patients undergoing PCI for acute coronary syndromes (ACS) or stable coronary artery disease (CAD). Patients were enrolled at 40 centers in the US, Canada, South Korea, and Mexico from May 2013 through October 2018; final date of follow-up was October 2019. <h3>Interventions</h3> Patients randomized to the genotype-guided group (n = 2652) underwent point-of-care genotyping.<i>CYP2C19</i>LOF carriers were prescribed ticagrelor and noncarriers clopidogrel. Patients randomized to the conventional group (n = 2650) were prescribed clopidogrel and underwent genotyping after 12 months. <h3>Main Outcomes and Measures</h3> The primary end point was a composite of cardiovascular death, myocardial infarction, stroke, stent thrombosis, and severe recurrent ischemia at 12 months. A secondary end point was major or minor bleeding at 12 months. The primary analysis was in patients with<i>CYP2C19</i>LOF variants, and secondary analysis included all randomized patients. The trial had 85% power to detect a minimum hazard ratio of 0.50. <h3>Results</h3> Among 5302 patients randomized (median age, 62 years; 25% women), 82% had ACS and 18% had stable CAD; 94% completed the trial. Of 1849 with<i>CYP2C19</i>LOF variants, 764 of 903 (85%) assigned to genotype-guided therapy received ticagrelor, and 932 of 946 (99%) assigned to conventional therapy received clopidogrel. The primary end point occurred in 35 of 903<i>CYP2C19</i>LOF carriers (4.0%) in the genotype-guided therapy group and 54 of 946 (5.9%) in the conventional therapy group at 12 months (hazard ratio [HR], 0.66 [95% CI, 0.43-1.02];<i>P</i> = .06). None of the 11 prespecified secondary end points showed significant differences, including major or minor bleeding in<i>CYP2C19</i>LOF carriers in the genotype-guided group (1.9%) vs the conventional therapy group (1.6%) at 12 months (HR, 1.22 [95% CI, 0.60-2.51];<i>P</i> = .58). Among all randomized patients, the primary end point occurred in 113 of 2641 (4.4%) in the genotype-guided group and 135 of 2635 (5.3%) in the conventional group (HR, 0.84 [95% CI, 0.65-1.07];<i>P</i> = .16). <h3>Conclusions and Relevance</h3> Among<i>CYP2C19</i>LOF carriers with ACS and stable CAD undergoing PCI, genotype-guided selection of an oral P2Y12 inhibitor, compared with conventional clopidogrel therapy without point-of-care genotyping, resulted in no statistically significant difference in a composite end point of cardiovascular death, myocardial infarction, stroke, stent thrombosis, and severe recurrent ischemia based on the prespecified analysis plan and the treatment effect that the study was powered to detect at 12 months. <h3>Trial Registration</h3> ClinicalTrials.gov Identifier:NCT01742117