tbuckley/cabot-search huggingface dataset:
Abstract 15007: Chemical Enhancement of Direct Cardiac Reprogramming in vitro and in vivo
Authors: Tamer M Mohamed, Nicole Stone, Emily Berry, Ethan Radzinsky, Yu Huang, Karishma Pratt, Yen-Sin Ang, Pengzhi Yu, Haixia Wang, Shibing Tang, Sergey Magnitsky, Sheng Ding, Kathryn Ivey, Deepak Srivastava
Journal: Circulation (2016)

Reprogramming of cardiac fibroblasts into induced cardiomyocyte-like cells (iCMs) in situ represents a promising strategy for cardiac regeneration. A combination of three cardiac transcription fact...
Evaluating Noninvasive Markers to Identify Advanced Fibrosis by Liver Biopsy in HBV/HIV Co‐infected Adults
Authors: Richard K. Sterling, Wendy C. King, Abdus S. Wahed, David E. Kleiner, Mandana Khalili, Mark Sulkowski, Raymond T. Chung, Mamta K. Jain, Mauricio Lisker‐Melman, David K. Wong, Marc G. Ghany
Journal: Hepatology (2019)

Noninvasive biomarkers are used increasingly to assess fibrosis in patients with chronic liver disease. We determined the utility of dual cutoffs for noninvasive biomarkers to exclude and confirm advanced fibrosis in hepatitis B virus (HBV)–human immunodeficiency virus (HIV) co‐infected patients receiving combined antiretroviral therapy. Participants were anti‐HIV/hepatitis B surface antigen–positive adults from eight clinical sites in the United States and Canada of the Hepatitis B Research Network. Fibrosis was staged by a central pathology committee using the Ishak fibrosis score (F). Clinical, laboratory, and vibration‐controlled transient elastography (VCTE) data were collected at each site. Dual cutoffs for three noninvasive biomarkers (aspartate aminotransferase‐to‐platelet ratio index, Fibrosis‐4 index [FIB‐4], and liver stiffness by VCTE) with the best accuracy to exclude or confirm advanced fibrosis (F ≥ 3) were determined using established methodology. Of the 139 enrolled participants, 108 with a liver biopsy and having at least one noninvasive biomarker were included: 22% had advanced fibrosis and 54% had normal alanine aminotransferase. The median (interquartile range) of APRI (n = 106), FIB‐4 (n = 106), and VCTE (n = 63) were 0.34 (0.26‐0.56), 1.35 (0.99‐1.89), and 4.9 (3.8‐6.8) kPa, respectively. The area under the curve for advanced fibrosis was 0.69 for APRI, 0.66 for FIB‐4, and 0.87 for VCTE. VCTE cutoffs of 5.0 kPa or less (to exclude) and 8.8 kPa or greater (to confirm) advanced fibrosis had a sensitivity of 92.3% and specificity of 96.0%, respectively, and accounted for 65.1% of participants. Among the 34.9% with values between the cutoffs, 26.1% had advanced fibrosis. Considering APRI or FIB‐4 jointly with VCTE did not improve the discriminatory capacity. Conclusion: VCTE is a better biomarker of advanced fibrosis compared with APRI or FIB‐4 in HBV/HIV co‐infected adults on combined antiretroviral therapy. Using VCTE dual cutoffs, approximately two‐thirds of patients could avoid biopsy to determine advanced fibrosis.
AB1085 OSTEO-ARTICULAR MANIFESTATIONS OF SARCOIDOSIS: ABOUT 13 CASES
Authors: ACHEK Mohamed Amine EL, Khadija Baccouche, Amel Farhat, Nejla El Amri, Hela Zeglaoui, Elyes Bouajina
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Sarcoidosis is a systemic disorder of unknown etiology, affecting the respiratory tract and the lymphatic system with predilection. It is an ubiquitous condition whose clinical phenotype is very diverse. <h3>Objectives</h3> To detail the epidemiological, clinico-biological, therapeutic and evolutionary features of osteoarticular forms of sarcoidosis. <h3>Methods</h3> This is a monocentric, descriptive retrospective study conducted over a 17-years period [2002-2018] involving 13 patients diagnosed with sarcoidosis with osteoarticular manifestations at the department of Rheumatology at Farhat Hached University Hospital in Sousse. <h3>Results</h3> 13 patients with osteoarticular manifestations were studied; this was indicative of the disease in 8 cases (61.5%). The sex ratio was 0.23 with a mean age of 42.38 years and a diagnostic delay of 12 months. In 77% of cases, inflammatory arthralgia was noted, Löfgren syndrome (30.7%), chronic polyarthritis (30.7%), phalanges cystoid osteitis (15.4%) and no cases of monoarthritis. The extra articular manifestations found were; Adenomegaly (92.3%), lung (77%), cutaneous (38.5%) (including 80% erythema nodosum), neurological (30.7%) (Aseptic meningitis and convulsion), ocular (23%) (chorioretinitis, keratoconjunctivitis and retro-bulbar optic neuritis), cardiac (15.4%), and biological hepatitis (7.7%). In the biology, a biological inflammatory syndrome was found in 30.7% with a mean sedimentation rate of 31.07 in the 1st hour, an average CRP of 9 mg/L, cholestasis and cytolysis found in 15.4% and 7.7%, respectively. No Hypercalcemia was noted. The value of the conversion enzyme was high in 5 out of 6 patients who were dosed with an average of 71 μg/L. In imaging, sarcoidosis was destructive in 4 cases; A multifocal osteonecrosis, Algodystrophy of the two hips and two cases of bullous cystoid osteitis of phalanges. <h3>Conclusion</h3> The osteo-articular involvement of sarcoidosis is polymorphic, which can reveal the disease or enamel its evolution. <h3>Disclosure of Interests</h3> None declared
Abstract 20266: Five-Year Clinical and Angiographic Outcome After Primary Stenting of Totally Occluded Native Coronary Arteries (PRISON II- Late Study) : A Randomized Comparison of Bare Metal Stent Implantation With Sirolimus-Eluting Stent Implantation for the Treatment of Total Coronary Occlusions
Authors: Ben J Van den Branden, Braim M Rahel, Gerrit J Laarman, Ton Slagboom, Johannes C Kelder, Jurrien M ten Berg, Maarten J Suttorp
Journal: Circulation (2010)

Objectives: The purpose of this study was to examine the five-year clinical and angiographic outcome in patients enrolled in the Primary Stenting of Totally Occluded Native Coronary Arteries II (PR...
THU0712-HPR QUALITY OF LIFE OF PATIENTS WITH MULTIPLE SCLEROSIS AND SYSTEMIC SCLEROSIS SUBMITTED TO HSCT: A COMPARATIVE AND LONGITUDINAL STUDY
Authors: Érika Arantes de Cardoso, Ana Luisa Carvalho Guimarães, Juliana Tomé Garcia Mareze, Lucas Dos Santos Lotério, Breno César Almeida Silva, Karla Costa Pereira, Belinda Pinto Simões, Maria Carolina Oliveira, Manoel Antônio Santos
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Multiple Sclerosis (MS) and Systemic Sclerosis (SSc) are chronic autoimmune diseases that comprise physical and psychological aspects. Disease progression is usually associated with a continuous decline in quality of life. In this devastating scenario, hematopoietic stem cell transplantation (HSCT) has emerged as an alternative treatment, improving physical outcomes and, at the same time, restoring a more normal life. Quality of life evaluations may be used to measure the effects of therapeutic interventions, considering their impact on patient life in the medium and long term. <h3>Objectives</h3> To evaluate the impact of HSCT on the Quality of Life (QoL) of SSc (G1) and MS (G2). <h3>Methods</h3> In this comparative, longitudinal, cross-sectional study, 56 patients (22 SSc and 34 MS) were included. The Medical Outcomes Study Short-Form 36 (MOS SF-36) scale, used for data collection, mainly assesses the physical health component (PHC) and the mental health component (MHC). The PHC encompasses the following domains: physical functioning (PF), role limitations due to physical problems (RPF), bodily pain (P) and general health perceptions (GHP). The MHC comprises: vitality (VIT), social functioning (SRF), role limitations due to emotional role functioning (ERF) and general mental health (MH). The results of each domain range from 0 to 100, where zero represents the worst and 100 the best state of health. Data were collected at admission and at 2-years after transplantation. Results underwent statistical analysis. Significance levels were established at p&lt;0.05. <h3>Results</h3> The study included intragroup comparisons (Pre<i> vs</i> post-HSCT; Table 1) and intergroup comparisons (G1 <i>vs</i> G2; Table 2) In the intragroup analyses, comparing pre and post-HSCT scores, there was general improvement in both disease groups, especially in G2. The intergroup comparisons indicate that before HSCT patients in G1 had better FP than those in G2 (p = 0.03). The other aspects were not significantly different between groups. After HSCT, patients in G2 had a better QoL scores in P (p = 0.4338), GHP (p = 0.0001) and MH (p = 0.0049). <h3>Conclusion</h3> These results may be interpreted as positive outcomes of HSCT for MS and SSc. However, our data indicate that HSCT may benefit patients with MS (G2) more than those with SSc (G1). <h3>References</h3> [1] Guimarães FAB, et al. Impact of autologous HSCT on the quality of life of patients with multiple sclerosis. Arquivos de Neuro-Psiquiatria, v. 68, p. 522-527, 2010. <h3>Disclosure of Interests</h3> None declared
Implications of different guidelines for surveillance after serrated polyp resection in United States of America and Europe
Authors: Arne Bleijenberg, Dagmar Klotz, Magnus Løberg, Evelien Dekker, Hans Olov Adami, Ernst J. Kuipers, Øyvind Holme, Mette Kalager, Louise Emilsson, Britta Kleist, Leif Løvdal, Jaroslaw Regula, Else M. Løberg, Joep IJspeert, Michael Bretthauer
Journal: Endoscopy (2019)

Abstract Introduction Because individuals with serrated polyps and adenomas are at increased risk of developing new polyps and colorectal cancer (CRC), surveillance after resection is justified. After adenoma resection, most international guidelines are consistent, but recommendations for surveillance after serrated polyp resection vary. The United States Multi-Society Taskforce on CRC (US-MSTF) base surveillance intervals on serrated polyp subtype (traditional serrated adenoma, sessile serrated polyp, hyperplastic polyps), while the European Society of Gastrointestinal Endoscopy (ESGE) guidelines do not take serrated polyp subtype into account. We evaluated the implications of this difference in a primary colonoscopy screening cohort. Methods We included participants from a large colonoscopy screening trial. In a post-hoc simulation, assuming full protocol adherence, we determined the surveillance interval for each subject based on their polyp burden, using the most recent US-MSTF and ESGE guidelines. Results We included 5323 participants, of whom 1228 had one or more serrated polyps. In 5201 of all participants (98 %; Cohen’s kappa 0.90) and in 1106 of those with serrated polyps (90 %; Cohen’s kappa 0.80), both guidelines recommended identical surveillance intervals. Recommendations for a 3-year surveillance interval were identical between the two guidelines. All 122 subjects with discordant recommendations would receive a follow-up colonoscopy after 10 years using ESGE guidance and after 5 years using US-MSTF guidance. Conclusion Despite the different criteria used to determine surveillance after serrated polyp resection, most individuals are recommended identical colonoscopy surveillance intervals whether following the ESGE or US-MSTF guidelines. This suggests that surveillance recommendations do not need to consider the serrated polyp subtype.
AB0859 THE SERUM CREATININE TO CYSTATIN C RATIO; A POSSIBLE NEW SURROGATE MARKER FOR BONE MINERAL DENSITY
Authors: Ichiro Yoshii, Tatsumi Chijiwa, Naoya Sawada
Journal: Annals of the Rheumatic Diseases (2019)

<h3>Background</h3> Bone mineral density (BMD) measured using dual-energy X-ray absorptiometry (DEXA) is one of the most important markers for osteoporosis diagnosis. The serum creatinine-to-cystatin C ratio (Cr/CysC) is in focus as a sarcopenia marker because it represents total muscle volume. Recently, Cr/CysC was suggested to have correlation with bone fragility fracture risk [1]. <h3>Objectives</h3> This study aimed to assess whether Cr/CysC is correlated with BMD. <h3>Methods</h3> BMD at the lumbar spine (LS) and femoral neck (FN) were measured in women diagnosed with postmenopausal osteoporosis. Steroid induced osteoporosis patients were eliminated. Cr/CysC was assessed at the time. Clinical factors, such as age, concurrent rheumatoid arthritis treatment (RA), concomitant biological drug administration (BIO), body mass index (BMI), concurrent dementia treatment (dementia), number of comorbidities other than dementia (N.Com), frailty score (FS), bone fragility fracture (BFF), and drug intervention for osteoporosis (DI), were evaluated. Multivariate linear regression (MLR) was used to assess the correlation of these factors with BMD at each part. The relationships between Cr/CysC and other parameters were statistically evaluated using MLR. Additionally, the relationships between the BMD change at each part and parameters were evaluated in the same manner. <h3>Results</h3> The study enrolled 207 subjects. BMD at the LS was significantly correlated with BMI and N.Com (p&lt;0.01), whereas BMD at the FN was significantly negatively correlated with age and was positively correlated with BMI, BFF, Cr/CysC, and DI (p&lt;0.01). Cr/CysC was significantly correlated with age, RA, BIO, dementia, FS, and BFF (p&lt;0.01). After effect of age was corrected, BMD at the FN demonstrated significant positive correlation with Cr/CysC (p&lt;0.01), whereas no statistical correlation demonstrated between BMD at the LS and Cr/CysC, Both BMD at the LS and the FN demonstrated significant positive correlation with BMI (p&lt;0.05). No parameter significantly correlated with the BMD change at both the LS and FN (p&lt;0.01). <h3>Conclusion</h3> Cr/CysC represents BMD at the FN and is a candidate for BMD substitution. <h3>References</h3> [1] Komorita Y, et al. Diabetes Res Clin Pract2018;146:202-10. <h3>Acknowledgement</h3> The author would like to thank Saori Tamura for the enthusiastic DXA and BMD measurements and Kaoru Kuwabara, Sayori Masuoka, and Mariko Osaki for their dedicated data collection <h3>Disclosure of Interests</h3> None declared
Abstract 18747: Young Infants With Severe Tetralogy of Fallot: Early Primary Surgery versus Trans-catheter Palliation
Authors: Travis J Wilder, Glen S Van Arsdell, Lee Benson, An Duong, Eric Pham-Hung, Michael Gritti, Alexandra Page, Christopher A Caldarone, Edward J Hickey
Journal: Circulation (2015)

Introduction: Infants with severe tetralogy of Fallot (TOF) may undergo: 1) early primary surgical repair (EARLY) or 2) early catheter palliation (CATH) prior to delayed surgical repair. We compared these two strategies to 3) usual elective single stage TOF repair (USUAL). Methods: We studied 453 TOF repairs (2000-2012, excluding BT shunts). USUAL strategy at our institution is repair ≥3 months. Risk adjusted hazard analysis compared freedom from surgical or catheter reintervention. Somatic size, branch PA size and RV systolic pressure were modeled using 2543 echo reports via mixed model regression. Results: Table: group characteristics for USUAL (383), EARLY (42) and CATH (28). CATH involved: RVOT stent=18, RVOT balloon=9, ductal stent=1. Risk adjusted freedom from surgical reoperation was 88%, 87% and 85% for USUAL, EARLY and CATH respectively, at 10 years. EARLY and CATH had similar reoperation rates, except for very young children (&lt;1 month) where EARLY primary repair conferred increased risk of further surgery (Figure). Risk adjusted freedom from catheter reintervention was higher for EARLY (76%) and especially so for CATH (53%) at 10 years, versus USUAL (83%; Figure). Somatic growth and progression of RVSP (37-40mmHg) was similar among groups at 8 years. EARLY (P=.02) and CATH (P=.09) tend to have smaller bPAs initially. The CATH group tend to remain smaller in the long-term, whereas growth in EARLY matches USUAL. Conclusions: Early primary repair at very young ages comes with a cost of increased late surgical reoperation. Early transcatheter palliation tends to come with a cost of increased late transcatheter intervention - possibly related to slower branch PA growth.
PS1228 SF3B1 KNOCKDOWN AT EARLY ERYTHROPOIESIS INDUCED CELL APOPTOSIS BY USING A TET‐ON LENTIVIRAL VECTOR
Authors: Y. Huang, X. Wu, L. Sun, X. An
Journal: HemaSphere (2019)

Background: SF3B1 has been drawing great interests of scientists due to their significantly high mutation frequency in one kind of hematological disease Myelodysplastic syndromes‐Refractory anemia with ring sideroblasts (MDS‐RARS), which is characterized by the anemia in clinic. However, the physiological roles of SF3B1 in erythropoiesis are poorly studied. Aims: To elucidate the biological functions of SF3B1 in specific stages of human erythropoiesis through precise temporal control of SF3B1 expression. To test the efficiency and safety of the new TET‐on lentiviral vector in differentiating cells. Methods: TET‐on lentiviral vector was constructed for conditional gene knockdown. Undifferentiated CD34+ cells were transduced with the Lentivirus and SF3B1 gene knockdown was induced by doxycycline in different differentiation stages across human erythropoiesis. The efficiency and safety of the TET‐on lentiviral vector were assessed by monitoring green fluorescent protein expression and erythroblasts differentiation, respectively. Knockdown of SF3B1 gene was checked by using qRT‐PCR and western blot analyses. The effects of SF3B1 ablation on progenitors were detected by colony forming assay and apoptosis was assessed by flow cytometry. Results: The TET‐on vector is efficient and safe for erythroblasts with no disturbance to the proliferation, differentiation, morphology and enucleation. Knockdown of SF3B1 in erythroblasts impairs erythroid colony forming and induced cell apoptosis. Interestingly, knockdown of SF3B1 shows temporal effects on erythropoiesis: in early stages, the deficiency of SF3B1 leads to significant cell apoptosis; however, it has no effect on cell growth at late stages of erythropoiesis. Summary/Conclusion: Our findings indicate that the TET‐on lentiviral vector is a powerful tool for precise temporal control of gene expression in differentiating cells, and SF3B1 is critical for the growth of early erythropoiesis.
PB2129 PRETREATMENT D‐DIMER LEVELS PROVIDE PROGNOSTIC INFORMATION IN PATIENTS WITH MULTIPLE MYELOMA
Authors: X. Peipei
Journal: HemaSphere (2019)

Background: High D‐dimer levels are associated with poor overall survival in patients with general cancer. Aims: This retrospective study aimed to investigate the prognostic value of D‐dimer levels in patients with multiple myeloma (MM). Methods A total of 214 patients newly diagnosed with MM were retrospectively enrolled. The correlation between pre‐treatment plasma D‐dimer levels and clinical factors was evaluated. The cut‐off value of D‐dimer for predicting survival was set as 0.8 μg/ml based on receiver operating curve analysis. Patients with a D‐dimer level ≥ 0.8 mg/ml had more adverse clinical features, including advanced R‐ISS stage, higher International Myeloma Working Group risk stratification, more abnormal chromosome, and increased serum creatinine level. The prognostic value of d‐dimer levels was explored. Results: Patients with a D‐dimer level ≥ 0.8 mg/ml showed poor overall survival (OS) and progression‐free survival (PFS). Multivariate analyses revealed that the D‐dimer levels were independent predictors of OS and PFS (hazard ratio = 10.15 and 0.27, 95% confidence interval = 4.86–21.19 and 0.18–0.42, P &lt; 0.001 and P &lt; 0.001). Summary/Conclusion: In conclusion, D‐dimer levels might be considered as a simple and effective prognostic marker in patients with MM.
Assessment of a Parent-Child Interaction Intervention for Language Development in Children
Authors: Dimitri A. Christakis, Sarah J. Lowry, Georgia Goldberg, Heather Violette, Michelle M. Garrison
Journal: JAMA Network Open (2019)

Parent-child interactions are critical for language development in early life.To test whether a clinic-based intervention was associated with improved home language environment and language development.A pre-post study of a multifaceted clinic-based intervention called Talk It Up was conducted for 61 English- or Spanish-speaking families with children aged 2 to 12 months. Enrollment took place at a single urban pediatric clinic from January to November 2017, with follow-up data collection through May 2018. Analyses took place from June to August 2018.The 6-month multifaceted intervention consisted of delivering Language Environment Analysis word counts with clinician feedback, coaching, and twice-weekly pushes of brief instructional videos via a commercially available smartphone application.Baseline and follow-up z scores for adult word counts, parent-child conversational turns, and child vocalizations and language development as measured by the Developmental Snapshot score were compared and adjusted for confounders.Among 61 families, the mean (SD) child's age at baseline was 5.9 (3.3) months. English was the primary language spoken in the home for 54 families (89%). At follow-up, Talk It Up was associated with significant improvements in adult word counts (mean z score, 0.30; 95% CI, 0.05-0.55), parent-child conversational turns (mean z score, 0.29; 95% CI, 0.002-0.59), and Developmental Snapshot score (mean, 6.59; 95% CI, 0.95-12.23), but there were no improvements in child vocalizations (mean z score, -0.13; 95% CI, -0.49 to 0.24).A multifaceted clinic-based approach to promote parent-child interactions holds some promise. Future, larger studies are warranted.
Abstract P050: Association Between Glycemic Control and Short-term Mortality Varies Across the Spectrum of Coronary Artery Disease
Authors: Sridharan Raghavan, Wenhui G Liu, P. Michael Ho, Mary E Plomondon, Anna E Baron, David Magid, Steven M Bradley, Thomas M Maddox
Journal: Circulation (2017)

Background: Diabetes is a significant risk factor for cardiovascular disease, but optimal glycemic control strategies remain unclear. In particular, trials of intensive glycemic control have highlighted a tension between increased mortality risk and macrovascular benefits. In this study we aimed to assess whether the burden of coronary artery disease (CAD) modifies the association between glycemic control and short-term mortality. Methods: We studied veterans with diabetes who underwent elective cardiac catheterization between 2005 and 2013 in a retrospective analysis of data from the VA Clinical Assessment, Reporting, and Tracking (CART) Program. Primary exposures were time-varying HbA1c over two years of follow-up after index catheterization, categorized as &lt;6%, 6-6.49%, 6.5-6.99%, 7-7.99%, 8-8.99%, and &gt;=9%, and burden of CAD, categorized as no CAD, non-obstructive CAD, or obstructive CAD. Primary outcome was two-year all-cause mortality. A total of 17394 participants had, on average, five HbA1c measurements over two years of follow-up. We used multivariable Cox proportional hazards regression to estimate the association between HbA1c and mortality, adjusting for demographic and clinical covariates and CAD burden, and including a term for interaction between HbA1c and CAD burden. Results: In adjusted models with 6.5 ≤ HbA1c ≤ 6.99% as the reference category, HbA1c &lt; 6% was associated with increased risk of mortality (HR 1.55 [1.25, 1.92]), whereas HbA1c categories above 7% were not. We observed significant interaction between glycemic control and CAD burden (interaction p=0.0005); the increased risk of short-term mortality at HbA1c &lt; 6% was limited to individuals with non-obstructive and obstructive CAD (Figure 1). Conclusions: HbA1c below 6% was associated with increased risk of short-term mortality, but only in individuals with CAD. CAD burden may thus inform individualized diabetes management strategies, specifically treatment de-escalation in individuals with any angiographically-defined CAD.