tbuckley/cabot-search huggingface dataset:
Abstract A20: Dysgeusia may play a role in negatively affecting mood and anxiety in post-radiation head and neck patients
Authors: Thomas Arnell, Quinn Self, Sharon Li, Carl Nelson
Journal: Clinical Cancer Research (2020)

Abstract Introduction: Anxiety and depression are well-documented comorbid conditions that many patients with head and neck cancer experience. Furthermore, radiation therapy for many head and neck cancers is associated with significant morbidity. Dysgeusia, in particular, is almost universally experienced with total taste loss occurring in up to 73% of individuals radiated for head and neck cancers. Prior studies have demonstrated a significant correlation between decreased quality of life and dysgeusia. In this retrospective study, we wish to further characterize the association between taste disturbance and patient-reported anxiety and mood. With better understanding of this relationship, we may be able to effectively intervene in improving the quality of life for head and neck patients. Methods: Forty-nine patients at The University of Vermont Medical Center who completed radiation therapy for head and neck cancer completed the University of Washington Quality of Life (QOL) Questionnaire at scheduled follow-up visits. Surveys were reviewed retrospectively for QOL measures, specifically patient-reported anxiety, mood, and taste. Results: Taste scores were the lowest at follow-up weeks 0-3 and more than tripled by the last follow-up, at 48 weeks (p&amp;gt;0.0001). The trend in taste improvement was most rapid during the first few weeks after radiation, but leveled off after 12 weeks. Mood and anxiety similarly improved across weeks after radiation when compared against taste. Conclusion: Our data suggest a correlation between taste disturbance, mood, and anxiety in patients after head and neck radiation. This suggests that targeting treatments to improve patients’ sense of taste post-radiation may improve their psychiatric well-being. Citation Format: Thomas Arnell, Quinn Self, Sharon Li, Carl Nelson. Dysgeusia may play a role in negatively affecting mood and anxiety in post-radiation head and neck patients [abstract]. In: Proceedings of the AACR-AHNS Head and Neck Cancer Conference: Optimizing Survival and Quality of Life through Basic, Clinical, and Translational Research; 2019 Apr 29-30; Austin, TX. Philadelphia (PA): AACR; Clin Cancer Res 2020;26(12_Suppl_2):Abstract nr A20.
Nonalcoholic Steatohepatitis—Reply
Authors: Adam C. Sheka, Bilal Hameed, Sayeed Ikramuddin
Journal: JAMA (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Pleural Thickness Correlates with Inflammatory Status, Vitronectin and Fibronectin Expression in the Acute Phase of Experimental Pleurodesis
Authors: L. Antonangelo, M. M. P. Acencio, L. R. Teixeira, V. L. Capelozzi, E. Marchi, F. S. Vargas
Journal: American Journal of Respiratory and Critical Care Medicine (2004)
Abstract 3008: A new mechanism of drug resistance in cancer: Extracellular ATP-induced resistance through ATP internalization and upregulation of ATP-binding cassette multidrug transporters
Authors: Haiyun Zhang, Xuan Wang, Xiaozhuo Chen
Journal: Cancer Research (2020)

Abstract Cancer is the second leading cause of death in the US. Multidrug resistance (MDR) and the resulting ineffectiveness of the drug treatment are responsible for most relapses of cancer and cancer-related death. One major mechanism of MDR is the efflux of multiple drugs mediated by ATP-binding Cassette (ABC) multidrug transporters. ABC transporters pump drugs with different structures and mechanisms of action out of cancer cells using ATP-provided energy. And ABC activities is also intimately linked to epithelial mesenchymal transition and metastasis. The opportunistic uptake of extracellular molecules has been named as a key emerging hallmark of cancer metabolism. Intratumoral extracellular ATP levels of various cancer types are 103 to 104 times higher than those in their corresponding normal tissues. Our previous studies have shown that extracellular ATP is internalized by cancer cell via macropinocytosis, and promotes cancer drug resistance by directly fueling ABC transporters and increase its drug efflux activities. However, whether extracellular ATP can induce change in the expression of ABC transporters and the underlying mechanisms remain unclear. Our results from quantitative reverse transcription PCR and flow cytometry show that extracellular ATP significantly alters mRNA levels and cell surface expression of ABC transporters. These results suggest that extracellular ATP has profound regulatory effects on ABC transporters and thus promotes drug resistance, linking the ATP-rich tumor microenvironment with cancer drug resistance. On-going studies include identification of mechanisms for the extracellular ATP-induced drug resistance via ABC transporters. The completion of the study will expand our understanding of the roles of extracellular ATP in cancer, and reveal new therapeutic targets for reducing drug resistance and enhancing anticancer efficacy of chemotherapeutics and targeted drugs. Citation Format: Haiyun Zhang, Xuan Wang, Xiaozhuo Chen. A new mechanism of drug resistance in cancer: Extracellular ATP-induced resistance through ATP internalization and upregulation of ATP-binding cassette multidrug transporters [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 3008.
Abstract 5133: Optimized RAS top-down proteomic assay reveals expanded proteoform landscape in malignant cells
Authors: Caroline DeHart, Kanika Sharma, Dominic Esposito, Anna Maciag, Dwight Nissley, Frank McCormick
Journal: Cancer Research (2020)

Abstract Traditional proteomic methods face an inherent challenge with the RAS family of GTPases (HRAS, NRAS, KRAS4A, KRAS4B). Expressed at low endogenous abundance, these four isoforms possess considerable sequence similarity, rendering the confident proteomic linkage of a given isoform with a specific mutation or post-translational modification (PTM) a formidable proposition outside of isoform-specific model cell lines. Conversely, top-down proteomic analysis (TDP), which measures intact protein molecules, can precisely identify modified protein forms, or proteoforms, and confidently localize both mutations and PTMs on each RAS isoform present. Previously, a method combining immunoprecipitation and subsequent TDP (IP-TDP) led to the identification of eleven KRAS4B proteoforms, including four mutations and two novel PTMs, within colorectal cancer cell lines and tumor samples†. These initial results demonstrated the great potential for proteoform-resolved measurements to further illuminate the role of KRAS4B mutations and modifications in human cancer. More recently, the NCI RAS Initiative has developed an enhanced RAS proteoform assay incorporating IP-TDP analysis on an Orbitrap Fusion Lumos mass spectrometer. By employing alternate antibodies, recombinant protein standards, and well-characterized model or malignant cell lines, we have obtained substantial improvements in both RAS proteoform detection and characterization, thus facilitating the identification and localization of low-abundance PTMs. Moreover, we have applied our optimized IP-TDP method to a diverse panel of malignant and RAS mutant cell lines with the goal of expanding our knowledge of the RAS proteoform landscape. In doing so, we have observed a wealth of novel RAS proteoforms, of far greater number and complexity than previously anticipated. We have also observed isoform-specific proteoform variations, most notably between KRAS4A and KRAS4B, and initial indications of proteoform context dependence, namely within a given mutation or tissue background. Our results further underscore the power of proteoform-resolved measurements and indicate that RAS-dependent signaling pathways in normal and cancer contexts may be far more complex than previously thought. † I Ntai et. al. “Precise characterization of KRAS4b proteoforms in human colorectal cells and tumors reveals mutation/modification crosstalk.” Proc Natl Acad Sci U S A. 2018 Apr 17; 115(16):4140-4145. Citation Format: Caroline DeHart, Kanika Sharma, Dominic Esposito, Anna Maciag, Dwight Nissley, Frank McCormick. Optimized RAS top-down proteomic assay reveals expanded proteoform landscape in malignant cells [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 5133.
Shared and distinct roles of T peripheral helper and T follicular helper cells in human diseases
Authors: Hiroyuki Yoshitomi, Hideki Ueno
Journal: Cellular and Molecular Immunology (2020)

The interactions of CD4+ T cells and B cells are fundamental for the generation of protective antibody responses, as well as for the development of harmful autoimmune diseases. Recent studies of human tissues and blood samples have established a new subset of CD4+ B helper T cells named peripheral helper T (Tph) cells. Unlike T follicular helper (Tfh) cells, which interact with B cells within lymphoid organs, Tph cells provide help to B cells within inflamed tissues. Tph cells share many B helper-associated functions with Tfh cells and induce B cell differentiation toward antibody-producing cells. The differentiation mechanism is also partly shared between Tph and Tfh cells in humans, and both Tfh and Tph cells can be found within the same tissues, including cancer tissues. However, Tph cells display features distinct from those of Tfh cells, such as the expression of chemokine receptors associated with Tph cell localization within inflamed tissues and a low Bcl-6/Blimp1 ratio. Unlike that of Tfh cells, current evidence shows that the target of Tph cells is limited to memory B cells. In this review, we first summarize recent findings on human Tph cells and discuss how Tph and Tfh cells play shared and distinct roles in human diseases.
Early short-course corticosteroids and furosemide combination to treat non-critically ill COVID-19 patients: An observational cohort study
Authors: Jean-Philippe Kevorkian, Jean-Pierre Riveline, Claire Vandiedonck, Diane Girard, Joris Galland, Florine Féron, Jean-François Gautier, Bruno Mégarbane
Journal: Journal of Infection (2020)
Weight Loss in Underserved Patients — A Cluster-Randomized Trial
Authors: Peter T. Katzmarzyk, Corby K. Martin, Robert L. Newton, John W. Apolzan, Connie L. Arnold, Terry C. Davis, Eboni G. Price-Haywood, Kara D. Denstel, Emily F. Mire, Tina K. Thethi, Phillip J. Brantley, William D. Johnson, Vivian Fonseca, Jonathan Gugel, Kathleen B. Kennedy, Carl J. Lavie, Daniel F. Sarpong, Benjamin Springgate
Journal: New England Journal of Medicine (2020)

Evidence of the effectiveness of treatment for obesity delivered in primary care settings in underserved populations is lacking.
Payer Mix Among Patients Receiving Dialysis
Authors: Eugene Lin, Kevin F. Erickson
Journal: JAMA (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Exploring Protein Space: From Hydrolase to Ligase by Substitution
Authors: Nir Hecht, Caroline L Monteil, Guy Perrière, Marina Vishkautzan, Eyal Gur
Journal: Molecular Biology and Evolution (2020)

The understanding of how proteins evolve to perform novel functions has long been sought by biologists. In this regard, two homologous bacterial enzymes, PafA and Dop, pose an insightful case study, as both rely on similar mechanistic properties, yet catalyze different reactions. PafA conjugates a small protein tag to target proteins, whereas Dop removes the tag by hydrolysis. Given that both enzymes present a similar fold and high sequence similarity, we sought to identify the differences in the amino acid sequence and folding responsible for each distinct activity. We tackled this question using analysis of sequence-function relationships, and identified a set of uniquely conserved residues in each enzyme. Reciprocal mutagenesis of the hydrolase, Dop, completely abolished the native activity, at the same time yielding a catalytically active ligase. Based on the available Dop and PafA crystal structures, this change of activity required a conformational change of a critical loop at the vicinity of the active site. We identified the conserved positions essential for stabilization of the alternative loop conformation, and tracked alternative mutational pathways that lead to a change in activity. Remarkably, all these pathways were combined in the evolution of PafA and Dop, despite their redundant effect on activity. Overall, we identified the residues and structural elements in PafA and Dop responsible for their activity differences. This analysis delineated, in molecular terms, the changes required for the emergence of a new catalytic function from a preexisting one.
Endoscopic stricturotomy for a refractory complex esophageal stricture
Authors: Amit Mehta, Shawn L. Shah, Srihari Mahadev
Journal: Endoscopy (2020)

Benign esophageal strictures present a frequent endoscopic challenge [1] [2] [3] [4]. Endoscopic stricturotomy using an electrocautery technique is effective for simple or short strictures (< 1 cm) [5]; however, there are few reports of its application for complex or long (> 1 cm) esophageal strictures.
Abstract 427: A mouse model for studying oncogenesis of HPV-associated oropharyngeal cancer
Authors: Eva McGhee, Yi-Ling Lin, Jay Vadgama, Khadijah Lang, Judith Okoro, Victoria Vidal, Naomi Long, Mengtao Li
Journal: Cancer Research (2020)

Abstract The human papillomavirus (HPV) causes 70% of oropharyngeal cancers in the United States. While the molecular and cellular mechanisms and the stepwise progression of HPV-related cervical cancer have been well studied, oropharyngeal HPV carcinogenesis remains to be unraveled since an equivalent HPV premalignant stage has never been identified in clinical oropharyngeal tissues. In this study, a series of keratinocyte cell lines derived from the base of the tongue of C3H mice were established. These cells formed varying degrees of malignancy in the syngenetic mice and are used to study the biology of HPV-related oropharyngeal cancer in an immunocompetent host. Keratinocytes from the base of the tongue were isolated from C3H newborns and were subsequently retrovirally immortalized with TERT or HPV16 E6E7 oncoproteins, with or without oncogene H-ras. They are designated MOKT1 (TERT), MOKT1/H-ras (TERT/H-ras), MOKE2 (E6E7), or MOKE2/H-ras (E6E7/H-ras). These cells were injected in the masseter muscle of syngeneic mice and the developing lesions were harvested for histological analysis over a time course of 1 month. E6E7 transformed MOKE2 cells exhibited features of HPV associated cancer cells. We found that p63 and phosphorylated STAT3 were upregulated in MOKE2 cells in comparison to MOKT1 cells. MOKE2 cells obtained growth advantages with increased cell proliferation and decreased apoptosis. In addition, MOKE2 cells showed increased cell migration activity in wound healing assays. While all cell lines grew indefinitely in vitro, MOKT1 and MOKT1/H-ras failed to show any sign of malignancy when implanted in vivo. MOKE2 demonstrated features of malignancy, but the phenotype was transient. Host immunity does not appear to be the determining factor for suppressing tumor growth as MOKE2 regressing also occurred when implanted in immunodeficient NSG mice. Only MOKE2/H-ras cells were capable of progressing and establishing permanent cancer. Ourdata suggest that differentiation induced cell growth arrest may contribute to tumor regression and a second hit is required to overcome such arrest. The MOKE2 cells can be used in syngeneic mice to study secondary oncogenic factors in the development of HPV-related oropharyngeal cancer. Citation Format: Eva McGhee, Yi-Ling Lin, Jay Vadgama, Khadijah Lang, Judith Okoro, Victoria Vidal, Naomi Long, Mengtao Li. A mouse model for studying oncogenesis of HPV-associated oropharyngeal cancer [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 427.
Results from a First-in-Human Study with Bnz-1: A Novel Peptide Inhibitor of IL-2, IL-9 and IL-15 for the Treatment of T-Cell Malignancies That Safely and Selectively Decreases Regulatory T-Cells, Natural Killer Cells, and CD8+ Central Memory T-Cells
Authors: Paul Frohna, Yutaka Tagaya, Anoshie Ratnayake, Nick Doerr, Asjad Basheer, Laith Al-Mawsawi, Woo Jae Kim, Juan Zapata, Xisorong Wu, Nazli Azimi
Journal: Blood (2017)
Targeting purine synthesis in ASS1-expressing tumors enhances the response to immune checkpoint inhibitors
Authors: Rom Keshet, Joo Sang Lee, Lital Adler, Muhammed Iraqi, Yarden Ariav, Lisha Qiu Jin Lim, Shaul Lerner, Shiran Rabinovich, Roni Oren, Rotem Katzir, Hila Weiss Tishler, Noa Stettner, Omer Goldman, Hadas Landesman, Sivan Galai, Yael Kuperman, Yuri Kuznetsov, Alexander Brandis, Tevi Mehlman, Sergey Malitsky, Maxim Itkin, S. Eleonore Koehler, Yongmei Zhao, Keyur Talsania, Tsai-wei Shen, Nir Peled, Igor Ulitsky, Angel Porgador, Eytan Ruppin, Ayelet Erez
Journal: Nature Cancer (2020)
Plasmacytoid dendritic cell expansion defines a distinct subset of <i>RUNX1</i>-mutated acute myeloid leukemia
Authors: Wenbin Xiao, Alexander Chan, Michael R. Waarts, Tanmay Mishra, Ying Liu, Sheng F. Cai, Jinjuan Yao, Qi Gao, Robert L. Bowman, Richard P. Koche, Isabelle S. Csete, Nicole L. DelGaudio, Andriy Derkach, Jeeyeon Baik, Sophia Yanis, Christopher A. Famulare, Minal Patel, Maria E. Arcila, Maximilian Stahl, Raajit K. Rampal, Martin S. Tallman, Yanming Zhang, Ahmet Dogan, Aaron D. Goldberg, Mikhail Roshal, Ross L. Levine
Journal: Blood (2020)
Epicardial Adipose Tissue Accumulation Confers Atrial Conduction Abnormality
Authors: Chrishan J. Nalliah, James R. Bell, Antonia J.A. Raaijmakers, Helen M. Waddell, Simon P. Wells, Gabriel B. Bernasochi, Magdalene K. Montgomery, Simon Binny, Troy Watts, Subodh B. Joshi, Elaine Lui, Choon Boon Sim, Marco Larobina, Michael O’Keefe, John Goldblatt, Alistair Royse, Geoffrey Lee, Enzo R. Porrello, Matthew J. Watt, Peter M. Kistler, Prashanthan Sanders, Lea M.D. Delbridge, Jonathan M. Kalman
Journal: Journal of the American College of Cardiology (2020)
THU0210 Clinical longterm updateable evaluation (clue) forms for clinical features and medications used in the management of patients with rheumatoid arthritis
Authors: T Sokka, T Pincus
Journal: Annals of the Rheumatic Diseases (2001)

<h3>Background</h3> Rheumatologists collect similar data in the care of patients with rheumatoid arthritis (RA) concerning clinical features, such as extra-articular disease, comorbidities, and classification criteria, as well as a history of therapies used in patient management. However, these data generally are collected in non-standard formats. Therefore, much clinical data are not easily accessible to the clinician in routine long-term care of individual patients, or to workers in clinical research concerning cohorts of patients at one centre or multiple centres. Advances in laboratory and clinical science are enhanced when data are collected in a standard format. <h3>Objectives</h3> To develop two clinical long term updateable evaluation (CLUE) standard forms for use in the management of patients with RA, one concerning clinical features, and the second concerning medication history, which can be maintained indefinitely at the front of the medical record. <h3>Methods</h3> Two one page CLUE forms were developed for use in usual clinical care. The RA clinical features CLUE form includes extra-articular disease, comorbidities, classification criteria for RA, possible family history of RA, records of joint surgeries/dates and radiographs. The medication review CLUE form includes all DMARDs and most current NSAIDs. Each form is designed to be updated easily at each visit, without a need to collect the data again. <h3>Results</h3> The CLUE forms have been used to characterise 102 consecutive patients with RA in routine clinical care. Medications in current use included prednisone (median dose 4 mg per day) by 94% of patients, methotrexate (median dose 15 mg per week) by 86%, hydroxycholoroquine by 28%, leflunomide by 10%, cyclosporine by 8%, etanercept by 8%, infliximab by 8%, auranofin by 3%, gold injections by 3%, azathioprine by 2%, penicillamine by 1%, and sulfasalazine by 1% of patients. Extra-articular disease included malaise reported by 72% of patients, dry mouth by 46%, carpal tunnel syndrome 37% dry eyes by 35%, and scleritis by 9% of patients at any time in the course of RA. Comorbities included chronic back pain in 36% of patients, hypertension in 31%, peptic ulcer in 27%, esophageal symptoms in 25%, cataract in 20%, thyroid disease in 11%, cancer in 10%, diabetes mellitus in 4%, renal disease in 3% of patients. Sixty-three percent of patients had smoked cigarettes and 28% were current smokers. <h3>Conclusion</h3> Two simple clinical longterm updateable evaluation (CLUE) forms are easily completed in usual clinical care to characterise patients with RA. These forms are complemented by a patient questionnaire and joint count form, to provide simple, standardised measures of RA for improved clinical care, clinical research, and clinical outcomes.