tbuckley/cabot-search huggingface dataset:
Curriculum for small-bowel capsule endoscopy and device-assisted enteroscopy training in Europe: European Society of Gastrointestinal Endoscopy (ESGE) Position Statement
Authors: Reena Sidhu, Stefania Chetcuti Zammit, Peter Baltes, Cristina Carretero, Edward J. Despott, Alberto Murino, Xavier Dray, David S. Sanders, Martin Keuchel, Evelien Dekker, James E. East, Gavin Johnson, Pedro Pimentel-Nunes, Marianna Arvanitakis, Thierry Ponchon, Mario Dinis-Ribeiro, Raf Bisschops
Journal: Endoscopy (2020)

Main Recommendations The European Society of Gastrointestinal Endoscopy (ESGE) has recognized the need to formalize training in small-bowel endoscopy across European centers. The following criteria and framework for training in small-bowel capsule endoscopy (SBCE) and device-assisted enteroscopy (DAE), which aim to provide uniform and high quality training to ensure that small-bowel endoscopists are competent to operate independently, are based on the current literature and experience of experts in the field. Three main areas are covered: skills required prior to commencing training in small-bowel endoscopy; structured training for trainees to become independent endoscopists; and ways of ensuring competence is achieved. 1 Centers providing training in SBCE should perform a minimum of 75 – 100 SBCEs/year. 2 Experience in bidirectional endoscopies is desirable for structured training in SBCE. 3 SBCE courses should consist of at least 50 % hands-on training and cover information on technology, indications and contraindications for SBCE, pathologies that can be encountered on SBCE, and standard terminology that should be used during reporting of SBCE. An SBCE course should be completed prior to achieving competence in SBCE reporting. 4 Competence in SBCE can be assessed by considering a minimum of 30 SBCEs. Direct Observation of Procedural Skills, short SBCE videos, and multiple-choice questions can be useful to assess improvement in the skills of trainees. 5 Centers offering training in DAE should aim to carry out at least 75 DAEs/year, should have direct links with an SBCE service, and should allow regular discussion of cases at a radiology small-bowel MDT. Training centers with lower numbers are encouraged to offer training by “buddying-up” with other centers, or using mentoring systems. 6 DAE trainees must be independent in bidirectional endoscopies and have experience in level 1 polypectomy prior to commencement of training. They should also be competent in reviewing SBCEs. 7 Training in DAE should be structured with a minimum of 75 procedures, including 35 retrograde DAEs, with therapeutic procedures undertaken in at least 50 % of the DAEs performed. Training should cover the indications, contraindications, complications including prevention, and technicalities of the DAE procedure; formal evaluation should follow. DAE trainees must acquire skills to independently manage and advise on small-bowel pathology following DAE procedures. 8 It is highly recommended that international societies develop online modules and courses on DAE, which are currently lacking across Europe.
Genome-Wide Association Study: Functional Variant rs2076295 Regulates Desmoplakin Expression in Airway Epithelial Cells
Authors: Yuan Hao, Samuel Bates, Hongmei Mou, Jeong H. Yun, Betty Pham, Jiangyuan Liu, Weiliang Qiu, Feng Guo, Jarrett D. Morrow, Craig P. Hersh, Christopher J. Benway, Lu Gong, Yihan Zhang, Ivan O. Rosas, Michael H. Cho, Jin-Ah Park, Peter J. Castaldi, Fei Du, Xiaobo Zhou
Journal: American Journal of Respiratory and Critical Care Medicine (2020)

Rationale: Genetic association studies have identified rs2076295 in association with idiopathic pulmonary fibrosis (IPF). We hypothesized that rs2076295 is the functional variant regulating DSP (desmoplakin) expression in human bronchial epithelial cells, and DSP regulates extracellular matrix–related gene expression and cell migration, which is relevant to IPF development.Objectives: To determine whether rs2076295 regulates DSP expression and the function of DSP in airway epithelial cells.Methods: Using CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 editing (including regional deletion, indel, CRISPR interference, and single-base editing), we modified rs2076295 and measured DSP expression in edited 16HBE14o- and primary airway epithelial cells. Cellular integrity, migration, and genome-wide gene expression changes were examined in 16HBE14o- single colonies with DSP knockout. The expression of DSP and its relevant matrix genes was measured by quantitative PCR and also analyzed in single-cell RNA-sequencing data from control and IPF lungs.Measurements and Main Results: DSP is expressed predominantly in bronchial and alveolar epithelial cells, with reduced expression in alveolar epithelial cells in IPF lungs. The deletion of the DNA region-spanning rs2076295 led to reduced expression of DSP, and the edited rs2076295GG 16HBE14o- line has lower expression of DSP than the rs2076295TT lines. Knockout of DSP in 16HBE14o- cells decreased transepithelial resistance but increased cell migration, with increased expression of extracellular matrix–related genes, including MMP7 and MMP9. Silencing of MMP7 and MMP9 abolished increased migration in DSP-knockout cells.Conclusions: rs2076295 regulates DSP expression in human airway epithelial cells. The loss of DSP enhances extracellular matrix–related gene expression and promotes cell migration, which may contribute to the pathogenesis of IPF.
Teamworking in endoscopy: a human factors toolkit for the COVID-19 era
Authors: Srivathsan Ravindran, Manmeet Matharoo, Mark Coleman, Sarah Marshall, Chris Healey, Ian Penman, Siwan Thomas-Gibson
Journal: Endoscopy (2020)

Abstract Background Endoscopy services have had to rapidly adapt their working practices in response to COVID-19. As recovery of endoscopy services proceeds, our workforce faces numerous challenges that can impair effective teamworking. We designed and developed a novel toolkit to support teamworking in endoscopy during the pandemic. Methods A human factors model was developed to understand the impact of COVID-19 on endoscopy teams. From this, we identified a set of key teamworking goals, which informed the development of a toolkit to support several team processes. The toolkit was refined following expert input and refinement over a 6-week period. Results The toolkit consists of four cognitive aids that can be used to support team huddles, briefings, and debriefs, alongside techniques to optimize endoscopic nontechnical skills across the patient-procedure pathway. We describe the processes that local endoscopy units can employ to implement this toolkit. Conclusion A toolkit of cognitive aids, based on human factors principles, may be useful in supporting teams, helping them adapt to working safely in the era of COVID-19.
METTL14-mediated N6-methyladenosine modification of SOX4 mRNA inhibits tumor metastasis in colorectal cancer
Authors: Xiaoxiang Chen, Mu Xu, Xueni Xu, Kaixuan Zeng, Xiangxiang Liu, Bei Pan, Chenmeng Li, Li Sun, Jian Qin, Tao Xu, Bangshun He, Yuqin Pan, Huilin Sun, Shukui Wang
Journal: Molecular Cancer (2020)

Colorectal cancer (CRC) is one of the leading causes of tumor-related death worldwide, and its main cause of death is distant metastasis. Methyltransferase-like 14(METTL14), a major RNA N6-adenosine methyltransferase, is involved in tumor progression via regulating RNA function. The goal of the study is to uncover the biological function and molecular mechanism of METTL14 in CRC.Quantitative real-time PCR (qRT-PCR), western blot and immunohistochemical (IHC) assays were employed to detect METTL14 and SOX4 in CRC cell lines and tissues. The biological functions of METTL14 were demonstrated using in vitro and in vivo experiments. Chromatin immunoprecipitation (ChIP), Transcrptomic RNA sequencing (RNA-Seq), m6A-RNA immunoprecipitation sequencing (MeRIP-Seq), RNA immunoprecipitation and luciferase reporter assays were used to explore the mechanism of METTL14 action.METTL14 expression was significantly downregulated in CRC and decreased METTL14 was associated with poor overall survival (OS). Both the univariate and multivariate Cox regression analysis indicated that METTL14 was an independent prognostic factor in CRC. Moreover, lysine-specific histone demethylase 5C(KDM5C)-mediated demethylation of histone H3 lysine 4 tri-methylation(H3K4me3) in the promoter of METTL14 inhibited METTL14 transcription. Functionally, we verified that METTL14 inhibited CRC cells migration, invasion and metastasis through in vitro and in vivo assays, respectively. Furthermore, we identified SRY-related high-mobility-group box 4(SOX4) as a target of METTL14-mediated m6A modification. Knockdown of METTL14 markedly abolished SOX4 mRNA m6A modification and elevated SOX4 mRNA expression. We also revealed that METTL14-mediated SOX4 mRNA degradation relied on the YTHDF2-dependent pathway. Lastly, we demonstrated that METTL14 might inhibit CRC malignant process partly through SOX4-mediated EMT process and PI3K/Akt signals.Decreased METTL14 facilitates tumor metastasis in CRC, suggesting that METTL14 might be a potential prognostic biomarker and effective therapeutic target for CRC.
Visualization of sodium dynamics in the kidney by magnetic resonance imaging in a multi-site study
Authors: James T. Grist, Frank Riemer, Esben S.S. Hansen, Rasmus S. Tougaard, Mary A. McLean, Joshua Kaggie, Nikolaj Bøgh, Martin J. Graves, Ferdia A. Gallagher, Christoffer Laustsen
Journal: Kidney International (2020)

Sodium magnetic resonance imaging (MRI) is a powerful, non-invasive technique to assess sodium distribution within the kidney. Here we undertook pre-clinical and clinical studies to quantify the corticomedullary sodium gradient in healthy individuals and in a porcine model of diuresis. The results demonstrated that sodium MRI could detect spatial differences in sodium biodistribution across the kidney. The sodium gradient of the kidney changed significantly after diuresis in the pig model and was independent of blood electrolyte measurements. Thus, rapid sodium MRI can be used to dynamically quantify sodium biodistribution in the porcine and human kidney.
Persistent positivity and fluctuations of SARS-CoV-2 RNA in clinically-recovered COVID-19 patients
Authors: Valeria Cento, Luna Colagrossi, Alice Nava, Anna Lamberti, Sabrina Senatore, Giovanna Travi, Roberto Rossotti, Marta Vecchi, Ornella Casati, Elisa Matarazzo, Alessandra Bielli, Giorgia Casalicchio, Maria Antonello, Silvia Renica, Valentino Costabile, Francesco Scaglione, Roberto Fumagalli, Nicola Ughi, Oscar Massimiliano Epis, Massimo Puoti, Chiara Vismara, Marino Faccini, Diana Fanti, Claudia Alteri, Carlo Federico Perno
Journal: Journal of Infection (2020)
Blockade of CTLA-4 increases anti-tumor response inducing potential of dendritic cell vaccine
Authors: Maryam Esmaily, Ali Masjedi, Shahin Hallaj, Mohsen Nabi Afjadi, Farinaz Malakotikhah, Sepideh Ghani, Armin Ahmadi, Mozhdeh Sojoodi, Hadi Hassannia, Fatemeh Atyabi, Afshin Namdar, Gholamreza Azizi, Ghasem Ghalamfarsa, Farhad Jadidi-Niaragh
Journal: Journal of Controlled Release (2020)
A single dose of doxycycline after an ixodes ricinus tick bite to prevent Lyme borreliosis: An open-label randomized controlled trial
Authors: M.G. Harms, A. Hofhuis, H. Sprong, S.C. Bennema, J.A. Ferreira, M. Fonville, A. Docters van Leeuwen, W.J.J. Assendelft, H.C.P.M. Van Weert, W. Van Pelt, C.C. Van den Wijngaard
Journal: Journal of Infection (2020)
Prehabilitation vs Postoperative Rehabilitation for Frail Patients
Authors: Jun Kako, Kohei Kajiwara, Noriatsu Tatematsu
Journal: JAMA Surgery (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
S100A6 is a critical regulator of hematopoietic stem cells
Authors: Tan Hooi Min Grahn, Abhishek Niroula, Ákos Végvári, Leal Oburoglu, Maroulio Pertesi, Sarah Warsi, Fatemeh Safi, Natsumi Miharada, Sandra C. Garcia, Kavitha Siva, Yang Liu, Emma Rörby, Björn Nilsson, Roman A. Zubarev, Stefan Karlsson
Journal: Leukemia (2020)

Abstract The fate options of hematopoietic stem cells (HSCs) include self-renewal, differentiation, migration, and apoptosis. HSCs self-renewal divisions in stem cells are required for rapid regeneration during tissue damage and stress, but how precisely intracellular calcium signals are regulated to maintain fate options in normal hematopoiesis is unclear. S100A6 knockout (KO) HSCs have reduced total cell numbers in the HSC compartment, decreased myeloid output, and increased apoptotic HSC numbers in steady state. S100A6KO HSCs had impaired self-renewal and regenerative capacity, not responding to 5-Fluorouracil. Our transcriptomic and proteomic profiling suggested that S100A6 is a critical HSC regulator. Intriguingly, S100A6KO HSCs showed decreased levels of phosphorylated Akt (p-Akt) and Hsp90, with an impairment of mitochondrial respiratory capacity and a reduction of mitochondrial calcium levels. We showed that S100A6 regulates intracellular and mitochondria calcium buffering of HSC upon cytokine stimulation and have demonstrated that Akt activator SC79 reverts the levels of intracellular and mitochondrial calcium in HSC. Hematopoietic colony-forming activity and the Hsp90 activity of S100A6KO are restored through activation of the Akt pathway. We show that p-Akt is the prime downstream mechanism of S100A6 in the regulation of HSC self-renewal by specifically governing mitochondrial metabolic function and Hsp90 protein quality.
Effects of Primary Biliary Cholangitis on Quality of Life and Health Care Costs in the United Kingdom
Authors: Stephen Rice, Viviana Albani, Dimitrios Minos, Gulnar Fattakhova, George F. Mells, Marco Carbone, Steven Flack, Nikoletta Varvaropoulou, Jonathan Badrock, Ann Spicer, Richard N. Sandford, Mark D.F. Shirley, Diarmuid Coughlan, Gideon Hirschfield, Simon D. Taylor-Robinson, Luke Vale, David E.J. Jones
Journal: Clinical Gastroenterology and Hepatology (2020)
Diagnostic Accuracy of Cardiac Computed Tomography and 18-F Fluorodeoxyglucose Positron Emission Tomography in Cardiac Masses
Authors: Emanuela Concetta D’Angelo, Pasquale Paolisso, Giovanni Vitale, Alberto Foà, Luca Bergamaschi, Ilenia Magnani, Giulia Saturi, Andrea Rinaldi, Sebastiano Toniolo, Matteo Renzulli, Domenico Attinà, Luigi Lovato, Giacomo Maria Lima, Rachele Bonfiglioli, Stefano Fanti, Ornella Leone, Maristella Saponara, Maria Abbondanza Pantaleo, Paola Rucci, Luca Di Marco, Davide Pacini, Carmine Pizzi, Nazzareno Galiè
Journal: JACC. Cardiovascular imaging (2020)
ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation
Authors: Yawei Liu, Robert Bockermann, Mahdieh Hadi, Iman Safari, Belinda Carrion, Marie Kveiborg, Shohreh Issazadeh-Navikas
Journal: Cellular and Molecular Immunology (2020)

A disintegrin and metalloproteinase (ADAM)12 was previously found to be expressed in T cells in the inflamed brain. However, the function of ADAM12 in T-cell responses in general and in tissue inflammation has not been examined. Here, we studied the role of ADAM12 in T-cell responses, fate determination on activation, and its functions in T cells to mediate tissue inflammation. We identified ADAM12 as a costimulatory molecule that is expressed on naive T cells and downregulated on stimulation. ADAM12 mimics CD28 costimulatory signaling to activate and induce the proliferation of T-helper 1 (Th1) cells. Monoclonal ADAM12 Fab antibodies trigger T-cell activation by amplifying TCR signaling to stimulate T-bet-mediated IFNγ production. Lack of genomic ADAM12 and its knockdown in T cells diminished T-bet and IFNγ production in Th1 cells, whereas other T cells, including Th17 cells, were unaffected. ADAM12 had similar functions in vivo on myelin antigen (MOG
Reperfusion of ST-Segment–Elevation Myocardial Infarction in the COVID-19 Era
Authors: Matthew J. Daniels, Mauricio G. Cohen, Anthony A. Bavry, Dharam J. Kumbhani
Journal: Circulation (2020)

e are in the midst of a generation-defining global pandemic, the scope, scale, and pace of which is unprecedented.Coronavirus 2019 (CO-VID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 virus), occurs in addition to existing challenges to emergency services, like ST-elevation myocardial infarction (STEMI).Both conditions may coexist, initial presentations can overlap, and true and reliable point-of-care testing does not exist.Furthermore, symptoms alone are unhelpful, as most screened for COVID-19 test negative (30% can be false negative) and ≈80% of COVID-19 infections are asymptomatic.Experts dealing with the COVID-19 epidemic in China recommend fibrinolytic therapy (FT) over primary percutaneous coronary intervention (PPCI) for STEMI.In this perspective, we analyze the arguments for a FT-based strategy in patients presenting with STEMI in the COVID-19 era.Collectively, we feel that this is a reasonable consideration, because fibrinolysis may be the best compromise of prompt reperfusion for the patient with the least resource implications for the institution, buying time for a complete clinical picture to be made.In caring for our patients, we must recognize that optimal care strategies, established outside the challenges of a pandemic, may be potentially suboptimal during one.
Interplay between persistent activity and activity-silent dynamics in the prefrontal cortex underlies serial biases in working memory
Authors: Joao Barbosa, Heike Stein, Rebecca L. Martinez, Adrià Galan-Gadea, Sihai Li, Josep Dalmau, Kirsten C. S. Adam, Josep Valls-Solé, Christos Constantinidis, Albert Compte
Journal: Nature Neuroscience (2020)
Abstract 12295: Characterization of a Novel Homozygous Multi-exon Ryr2 Duplication Associated With Exertion-related Sudden Unexplained Death in the Young in the Amish Community Using Patient-specific Hipsc-cardiomyocytes
Authors: David J Tester, CS John Kim, Samantha K Hamrick, Hannah M Bombei, Kristi K Fitzgerald, Carla M Haglund-Turnquist, Dianne L Atkins, Luis A Ochoa, Ian H Law, Joel Temple, Michael J Ackerman
Journal: Circulation (2019)

Background: We identified a novel homozygous duplication involving the promoter region and exons 1-4 of RYR2 that is responsible for highly penetrant, exertion-related sudden deaths in the young (S...
Optimized tandem CD19/CD20 CAR-engineered T cells in refractory/relapsed B cell lymphoma
Authors: Chuan Tong, Yajing Zhang, Yang Liu, Xingyu Ji, Wen-ying Zhang, Yelei Guo, Xiao Han, Dongdong Ti, Hanren Dai, Chunmeng Wang, Qingming Yang, Wanli Liu, Yao Wang, Zhiqiang Wu, Weidong Han
Journal: Blood (2020)
COVID-19 revisiting inflammatory pathways of arthritis
Authors: Georg Schett, Bernhard Manger, David Simon, Roberto Caporali
Journal: Nature Reviews Rheumatology (2020)

Coronavirus disease 2019 (COVID-19) is an infectious disease, caused by severe acute respiratory syndrome coronavirus 2, which predominantly affects the lungs and, under certain circumstances, leads to an excessive or uncontrolled immune activation and cytokine response in alveolar structures. The pattern of pro-inflammatory cytokines induced in COVID-19 has similarities to those targeted in the treatment of rheumatoid arthritis. Several clinical studies are underway that test the effects of inhibiting IL-6, IL-1β or TNF or targeting cytokine signalling via Janus kinase inhibition in the treatment of COVID-19. Despite these similarities, COVID-19 and other zoonotic coronavirus-mediated diseases do not induce clinical arthritis, suggesting that a local inflammatory niche develops in alveolar structures and drives the disease process. COVID-19 constitutes a challenge for patients with inflammatory arthritis for several reasons, in particular, the safety of immune interventions during the pandemic. Preliminary data, however, do not suggest that patients with inflammatory arthritis are at increased risk of COVID-19. This Perspective article explores similarities in the inflammatory processes underlying coronavirus disease 2019 (COVID-19) and rheumatoid arthritis, including the role of pro-inflammatory cytokines and the potential of anti-cytokine therapies to treat COVID-19, as well as the effect of the COVID-19 pandemic on rheumatology.
<i>ARID1A</i> mutation plus CXCL13 expression act as combinatorial biomarkers to predict responses to immune checkpoint therapy in mUCC
Authors: Sangeeta Goswami, Yulong Chen, Swetha Anandhan, Peter M. Szabo, Sreyashi Basu, Jorge M. Blando, Wenbin Liu, Jan Zhang, Seanu Meena Natarajan, Liangwen Xiong, Baoxiang Guan, Shalini Singh Yadav, Abdel Saci, James P. Allison, Matthew D. Galsky, Padmanee Sharma
Journal: Science Translational Medicine (2020)

A combination of ARID1A mutation and CXCL13 expression in baseline tumor tissues improves predictive capability for patients with mUCC receiving ICT.