tbuckley/cabot-search huggingface dataset:
Contact — but not foul play — dominates injury mechanisms in men’s professional handball: a video match analysis of 580 injuries
Authors: Patrick Luig, Werner Krutsch, Thomas Henke, Christian Klein, Hendrik Bloch, Petra Platen, Leonard Achenbach
Journal: British Journal of Sports Medicine (2020)

We aimed to identify patterns and mechanisms of injury situations in men's professional handball by means of video match analysis.Moderate and severe injuries (absence of >7 days) sustained in competition in one of six seasons (2010 to 2013 and 2014 to 2017) in men's professional handball were prospectively analysed with a newly developed standardised observation form. Season 2013 to 2014 was excluded because of missing video material.580 injuries were identified: 298 (51.4%) contact injuries, 151 (26.0%) indirect contact injuries and 131 (22.6%) non-contact injuries. Head (87.5%), hand (83.8%), shoulder (70.2%) and ankle (62.9%) injuries were mainly sustained during direct contact. Typical contact injuries included collision with an opponent's upper extremity or torso, and ankle injuries mainly consisted of foot-to-foot collisions. A large proportion (41.7%) of knee injuries were caused by indirect contact, whereas thigh injuries mainly occurred (56.4%) through non-contact mechanism. Wing (56.9%) and pivot (58.4%) players had the highest proportion of contact injuries, whereas backcourt players had a high proportion of indirect contact injuries (31.5%) and goalkeepers of non-contact injuries (48.9%). The injury proportion of foul play was 28.4%. Most injuries occurred in the central zone between the 6-metre and 9-metre lines (26.1%) and during the last 10 min of each match half (OR 1.71, p=0.016).In men's professional handball in a league setting, contact - but not foul play - was the most common mechanism associated with moderate and severe injuries. Head, hand, shoulder and ankle injury were mainly sustained during direct contact.
Corrigendum re “Periurethral Injection of Autologous Adipose-Derived Stem Cells with Controlled-Release Nerve Growth Factor for the Treatment of Stress Urinary Incontinence in a Rat Model” [Eur Urol 2011;59:155–63]
Authors: Weiming Zhao, Cheng Zhang, Chengjun Jin, Zijian Zhang, Deling Kong, Wanhai Xu, Youcheng Xiu
Journal: European Urology (2020)
Reply to Gueret <i>et al.</i> and to Hammond <i>et al.</i>
Authors: Ryan M. Brown, Li Wang, Jonathan D. Casey, Karen E. Jackson, Wesley H. Self, Todd W. Rice, Matthew W. Semler
Journal: American Journal of Respiratory and Critical Care Medicine (2020)

A recently published systematic review and meta-analysis attempted to clarify the impact of using balanced crystalloids in sepsis; however, including these new data with previously published controlled trials and observational studies improves on those efforts (2).When prior data are considered alongside the SMART post hoc analysis, the benefit on short-term mortality appears less certain, although potentially still impactful (odds ratio [OR], 0.88; 95% confidence interval [CI], 0.76-1.01)(1, 3-5) (Figure 1).We have greater confidence in the decreased odds of patients developing acute kidney injury (OR, 0.67; 95% CI, 0.49-0.92)but uncertainty regarding progression to receipt of renal replacement therapy (OR, 0.85; 95% CI, 0.71-1.03)when receiving balanced crystalloids (1, 4-6).Although two of the three individual outcomes did not show significance, there was a lower incidence of major adverse kidney events in 30 days with balanced crystalloids compared with saline (OR, 0.78; 95% CI, 0.65-0.94) in the two studies that evaluated this outcome (1,6).Differences in this composite outcome, which are not being driven by a particular intermediate clinical endpoint, are extremely encouraging for balanced crystalloid use.When financial costs are considered, the proposition of using a balanced crystalloid rather than saline becomes even more attractive.Although Plasma-Lyte 148 is almost three times more costly than saline, lactated Ringer's is available at approximately the same purchase price as saline.The majority of patients in most published studies who were prescribed a balanced crystalloid received lactated Ringer's (1, 4-6).Given the significant costs associated with poor clinical outcomes and adverse events during critical illness, using lactated Ringer's in critically ill patients with sepsis is likely to be a substantially cost-effective intervention in most scenarios.We hope that the fine work by Brown and colleagues will be considered favorably alongside other published evaluations of balanced crystalloids versus saline in critically ill adults (1).At this time, we believe providing fluid resuscitation with balanced crystalloids, particularly lactated Ringer's, to adult patients with sepsis is likely a more effective and cost-effective intervention than saline.
Conditional reprogramming: next generation cell culture
Authors: Xiaoxiao Wu, Shengpeng Wang, Mingxing Li, Jing Li, Jing Shen, Yueshui Zhao, Jun Pang, Qinglian Wen, Meijuan Chen, Bin Wei, Parham Jabbarzadeh Kaboli, Fukuan Du, Qijie Zhao, Chi Hin Cho, Yitao Wang, Zhangang Xiao, Xu Wu
Journal: Acta Pharmaceutica Sinica B (2020)

Long-term primary culture of mammalian cells has been always difficult due to unavoidable senescence. Conventional methods for generating immortalized cell lines usually require manipulation of genome which leads to change of important biological and genetic characteristics. Recently, conditional reprogramming (CR) emerges as a novel next generation tool for long-term culture of primary epithelium cells derived from almost all origins without alteration of genetic background of primary cells. CR co-cultures primary cells with inactivated mouse 3T3-J2 fibroblasts in the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling primary cells to acquire stem-like characteristics while retain their ability to fully differentiate. With only a few years' development, CR shows broad prospects in applications in varied areas including disease modeling, regenerative medicine, drug evaluation, drug discovery as well as precision medicine. This review is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its wide applications, highlighting the value of CR in both basic and translational researches and discussing the challenges faced with CR.
Targeting the two major isoforms of heat shock protein 70: A novel approach to inhibiting the molecular chaperone heat shock protein 90
Authors: Marissa V. Powers, Paul A. Clarke, Paul Wokman
Journal: Cancer Research (2006)

191  Hsp90 has emerged as an exciting anticancer drug target due to its role in maintaining the conformational stability of a plethora of oncogenic client proteins. The molecular chaperone function of Hsp90 is dependent on ATP hydrolysis which is orchestrated by interactions with a number of co-chaperones including Hsp70. 17-allylamino-17-demethoxygeldanamycin (17AAG) competitively inhibits the intrinsic ATPase of Hsp90 which we and others have previously demonstrated to cause cytostasis and cell line-dependent cell death in vitro, the extent of which is relatively small when compared to that induced by other clinically relevant agents such as 5-FU and oxaliplatin (Clarke et al unpublished observations). This may be due to induction of the major constitutive (Hsc70) and inducible (Hsp72) isoforms of the Hsp70 family, which we and others have previously shown to occur in response to Hsp90 inhibition. Since the Hsp70 family can have anti-apoptotic activity, we used an siRNA approach to reduce the expression of Hsp72 and/or Hsc70 in the human colon cancer cell line HCT116. Transient knockdown of Hsp72, but not Hsc70, prior to treatment with 17AAG caused an increase in the cell death response to this Hsp90 inhibitor. We hypothesized that the absence of increased cell death following Hsc70 reduction may be due to concurrent induction of Hsp72. To investigate this, the expression of the two isoforms were simultaneously reduced which resulted in increased cell death from 13.1% (± 2.18 s.e) to 40.3% (± 13.79 s.e) of the total cell population following only 24 hours exposure to 17AAG. In addition, we observed a time-dependent increase in cell death following reduction of Hsp72 and Hsc70 expression, in the absence of 17AAG exposure. This increase in cell death may be in part due to inhibition of Hsp90 since knockdown of Hsp72 and Hsc70 resulted in reduced expression of Hsp90 client proteins c-RAF, CDK4 and ErbB2. The observed reduction in Hsp90 client proteins was shown to involve ubiquitin-dependent proteasome degradation, as co-treatment with proteasome inhibitors bortezomib and MG-132 resulted in reduced degradation of these clients. In conclusion, we have demonstrated that induction of Hsp72 provides a cytoprotective effect against the cell death activity of 17AAG. In addition, reducing the expression of Hsc70 with Hsp72 produces a molecular phenotype similar to that of Hsp90 inhibition but with a degree of cell death far greater than is associated with existing Hsp90 inhibitors. This is consistent with an anti-apoptotic effect of Hsp72 and Hsc70, which makes the development of Hsp70 inhibitors an exciting prospect, both in terms of enhancing the cell death activity of existing Hsp90 inhibitors and also as anticancer therapeutics in their own right.
Subclonal NT5C2 mutations are associated with poor outcomes after relapse of pediatric acute lymphoblastic leukemia
Authors: Malwine J. Barz, Jana Hof, Stefanie Groeneveld-Krentz, Jui Wan Loh, Annabell Szymansky, Kathy Astrahantseff, Arend von Stackelberg, Hossein Khiabanian, Adolfo A. Ferrando, Cornelia Eckert, Renate Kirschner-Schwabe
Journal: Blood (2020)
Pediatric Implications of Opioid-Specific Parental Drug Use
Authors: Guan Wang, Wenquan Niu
Journal: JAMA Pediatrics (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Jeannette Alexandra Smith
Authors: Pamela J F Smith
Journal: BMJ (2020)

Jeannette Alexandra Ferguson was born on Guernsey, the daughter of Alexander and Emily Ferguson and the youngest of four girls. She stayed on the island during the German occupation, and the resilience and resourcefulness of her fellow islanders helped to shape her character. After studying for her A levels on the mainland she was accepted by the medical school at the University of Liverpool.

She joined the Methodist Society, where she became secretary and then president. …
B30 The Role of SMARCA4 as an EGFR-Independent Mechanism of Resistance to Osimertinib
Authors: F.J. de Miguel, B. Hu, W.L. Cai, N. Sun, M.C. Melnick, D.X. Nguyen, A.Z. Xiao, K.A. Politi
Journal: Journal of Thoracic Oncology (2020)
PUMA-GとHM74はニコチン酸の受容体であり,抗脂質分解作用に関与する | 文献情報 | J-GLOBAL 科学技術総合リンクセンター
Authors: Tunaru S, Kero J, Schaub A, Wufka C, Blaukat A, Pfeffer K, Offermanns S
Journal: Nature Medicine (2003)
The Missed Patient With Diabetes
Authors: Xuanping Zhang, Linda S. Geiss, Yiling J. Cheng, Gloria L. Beckles, Edward W. Gregg, Henry S. Kahn
Journal: Diabetes Care (2008)

OBJECTIVE—This study examined the association between access to health care and three classifications of diabetes status: diagnosed, undiagnosed, and no diabetes. RESEARCH DESIGN AND METHODS—Using data from the 1999–2004 National Health and Nutrition Examination Survey, we identified 110 “missed patients” (fasting plasma glucose &amp;gt;125 mg/dl but without diagnoses of diabetes), 704 patients with diagnosed diabetes, and 4,782 people without diabetes among adults aged 18–64 years. The population percentage undetected among adults with diabetes and the odds ratio of being undetected among adults who reported not having diabetes were compared between groups based on their access to health care. RESULTS—Among those with diabetes, the percentages having undetected diabetes were 42.2% (95% CI 36.7–47.7) among the uninsured, 25.9% (22.9–28.9) among the insured, 49.3% (43.0–55.6) for those uninsured &amp;gt;1 year, 38.7% (29.2–48.2) for those uninsured ≤1 year, and 24.5% (21.7–27.3) for those continuously insured over the past year. Type of insurance, number of times receiving health care in the past year, and routine patterns of health care utilization were also associated with undetected diabetes. Multivariate adjustment indicated that having undetected diabetes was associated with being uninsured (odds ratio 1.7 [95% CI 1.0–2.9]) and with being uninsured &amp;gt;1 year (2.6 [1.4–5.0]). CONCLUSIONS—Limited access to health care, especially being uninsured and going without insurance for a long period, was significantly associated with being a “missed patient” with diabetes. Efforts to increase detection of diabetes may need to address issues of access to care.
Iannis Aifantis: An accidental scientist
Authors: Stephanie Houston
Journal: The Journal of Experimental Medicine (2020)

Iannis Aifantis is a principal investigator at NYU Langone Medical Center, and his laboratory works on the molecular mechanisms that drive normal stem cell differentiation and malignant transformation. Specifically, they’re interested in the genomic, epigenetic, and proteomic regulation of hematopoietic stem cell differentiation and the induction of leukemia and lymphoma; some of their basic research has led to clinical trials in leukemia and myelodysplastic syndromes. I chatted with Iannis to find out about his career in science so far.
Medical Assessment of Head Collision Events in Elite Women’s and Men’s Soccer
Authors: Christopher Tarzi, Gabriel Tarzi, Madison Walker, Olli Saarela, Michael D. Cusimano
Journal: JAMA (2020)

Medical Assessment of Head Collision Events in Elite Women's and Men's SoccerThe International Conference on Concussion in Sport protocols, which state that all athletes with suspected concussion must receive medical assessment, have been supported by elite soccer organizations. 1 However, a majority of male soccer players do not receive medical assessment after head collision events (HCEs). [2]3][4] To our knowledge no research has analyzed HCEs in elite women's soccer.The purpose of this study was to determine the incidence rate and subsequent medical assessment of HCEs in the 2019 Fédération Internationale de Football Association (FIFA) Women's World Cup and to compare these with rates from 3 elite men's tournaments. Methods |The 52 matches of the 2019 FIFA Women's World Cup were independently reviewed by 3 trained observers who recorded data on frequency and medical assessment of HCEs, time stopped for assessment, and visible concussion signs.Data were collected using the same standardized form as in prior HCE analyses. [2]3][4] The Cohen κ among reviewers was 0.88.Head collision events were defined as any incident in which a player was unable to resume play within 5 seconds of direct head contact.Medical assessment was any assessment on the field or sideline conducted by health care team personnel.Time stopped for assessment was the duration between the player receiving assessment and returning to the match.Players involved in HCEs were observed for the following visible signs of concussion: clutching of head, slowness getting up, disorientation, disequilibrium, loss of consciousness, and seizures.Data from 3 elite men's tournaments were combined for the comparative analysis. [2]3][4] All 4 studies were conducted in the same way using the same definitions.The Mann-Whitney U test was used for comparison of median time stopped for assessment.Categorical variables were compared using the χ 2 test or Fisher exact test as appropriate.Incidence comparisons were estimated by quasi-Poisson generalized linear models.Two-sided statistical significance was set at P < .05.R version 3.6.0(R Core Team 2019) was used for statistical analysis.
Community-Based Hemoglobin A<sub>1C</sub> Testing in Barbershops to Identify Black Men With Undiagnosed Diabetes
Authors: Marcela Osorio, Joseph E. Ravenell, Mary A. Sevick, Yonathan Ararso, Ta’Loria Young, Stephen P. Wall, David C. Lee
Journal: JAMA Internal Medicine (2020)

This cross-sectional study evaluates the use of hemoglobin A1c testing at barbershops owned by black individuals for timely diagnosis of diabetes among black men and suggests appropriate methods for care.
Abstract 410: Early Imaging Incompletely Predicts Subsequent Cerebral Physiological Derangements After Cardiac Arrest
Authors: Francisco Gomez, Benjamin Abella, Ramani Balu
Journal: Circulation (2019)

Introduction: Decreased grey-white matter ratio (GWR) on early head CT scans correlates with poor outcome after cardiac arrest. However, it is unknown if early imaging features fully predict of subsequent derangments in cerebral physiology that cause secondary brain injury. Hypothesis: GWR on early head CT correlates with average intracranial pressure (ICP), brain tissue oxygen (PbtO2), and pressure reactivity (a proxy for cerebral autoregulation) after cardiac arrest. Methods: A total of 30 post-arrest patients were identified over a 3-year period who underwent head CT within 1 day of arrest as well as intracranial monitoring (IM) during hospitalization. 5 patients were excluded either due to unavailable IM recordings (n=1) or motion degraded head CTs (n=4). ICP, P bt O 2 , and mean arterial pressure (MAP) were recorded continuously using a bedside recording unit (CNS Monitor, Moberg Research Inc). Pressure reactivity index (PRx), was calculated as the Pearson correlation coefficient between ICP and MAP over 5 minute intervals, updated every minute. GWR was measured for the basal ganglia from Hounsfield unit measurements made from initial head CT. Relationships between average ICP, PRx, P bt O 2 and GWR was calculated using linear regression. Results: Average GWR for the cohort was 1.2154. Average ICP was weakly correlated with initial GWR (R 2 =0.257, p=0.01). A trend towards higher PRx values was seen with lower initial GWR; however, this did not reach statistical significance (R 2 =0.15, p=0.07). There was no relation between average P bt O 2 and GWR (R 2 =0.02, p=0.56). Conclusions: Lower initial GWR is associated with higher average ICP, and may also be associated with disorderd cerebral autoregulation. However, no relationship could be identified between initial imaging findings and average brain oxygen levels. These data imply that physiological stressors such as brain hypoxia are not predicted by initial imaging findings and suggests a role for invasive neuromonitoring to identify and treat processes that promote secondary brain injury.
Systemic Absorption of Sunscreen
Authors: Adewole S. Adamson, Kanade Shinkai
Journal: JAMA (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Paired analysis of TCR alpha and TCR beta chains at the single-cell level in mice
Authors: Pradyot Dash, Jennifer L McClaren, Thomas H Oguin, William Rothwell, Brandon Todd, Melissa Y Morris, Jared Becksfort, Cory Reynolds, Scott A Brown, Peter C Doherty, Paul G Thomas
Journal: Journal of Clinical Investigation (2011)

Characterizing the TCRα and TCRβ chains expressed by T cells responding to a given pathogen or underlying autoimmunity helps in the development of vaccines and immunotherapies, respectively. However, our understanding of complementary TCRα and TCRβ chain utilization is very limited for pathogen- and autoantigen-induced immunity. To address this problem, we have developed a multiplex nested RT-PCR method for the simultaneous amplification of transcripts encoding the TCRα and TCRβ chains from single cells. This multiplex method circumvented the lack of antibodies specific for variable regions of mouse TCRα chains and the need for prior knowledge of variable region usage in the TCRβ chain, resulting in a comprehensive, unbiased TCR repertoire analysis with paired coexpression of TCRα and TCRβ chains with single-cell resolution. Using CD8+ CTLs specific for an influenza epitope recovered directly from the pneumonic lungs of mice, this technique determined that 25% of such effectors expressed a dominant, nonproductively rearranged Tcra transcript. T cells with these out-of-frame Tcra mRNAs also expressed an alternate, in-frame Tcra, whereas approximately 10% of T cells had 2 productive Tcra transcripts. The proportion of cells with biallelic transcription increased over the course of a response, a finding that has implications for immune memory and autoimmunity. This technique may have broad applications in mouse models of human disease.
Plaque-associated myeloid cells derive from resident microglia in an Alzheimer’s disease model
Authors: Erin G. Reed-Geaghan, Andrew L. Croxford, Burkhard Becher, Gary E. Landreth
Journal: The Journal of Experimental Medicine (2020)

Alzheimer’s disease (AD) is accompanied by a robust inflammatory response mediated by plaque-associated myeloid cells of the brain. These cells exhibit altered gene expression profiles and serve as a barrier, preventing neuritic dystrophy. The origin of these cells has been controversial and is of therapeutic importance. Here, we genetically labeled different myeloid populations and unequivocally demonstrated that plaque-associated myeloid cells in the AD brain are derived exclusively from resident microglia, with no contribution from circulating peripheral monocytes.