Poster session 2Morphogenetic mechanisms290MiR-133 regulates retinoic acid pathway during early cardiac chamber specification291Bmp2 regulates atrial differentiation through miR-130 during early heart looping formationDevelopmental genetics294Association of deletion allele of insertion/deletion polymorphism in alpha 2B adrenoceptor gene and hypertension with or without type 2 diabetes mellitus295Association of G1359A polymorphism of the endocannabinoid type 1 receptor (CNR1) with coronary …
Authors: V Garcia-Martinez, C Lopez Sanchez, W Hamed, W Hamed, JH Hsu, R Ferrer-Lorente, Maryam Alshamrani, J Pizzicannella, C Vindis, I Badi, L Korte, C Voellenkle, L S Niculescu, M Massaro, AR Babaeva, F Da Silva, L Woudstra, A Berezin, MK Bae, C Del Giudice, S A Bageghni, K Krobert, M Levay, N Vignier, A Ranieri, A Magenta, A Orlandi, B Porro, E-S Jeon, Y Omori, J Herold, G A Barnett, A Grochot-Przeczek, P Korpisalo, C Deffge, A Margariti, W Rong, JA Maring, J Gambardella, CG Mitrofan, O Karpinska, L Morbidelli, FL Wilkinson, A Berezin, AS Kostina, J G R De Mey, A Kumar, A Lupieri, C Pellet-Many, R Stamatiou, A Gromotowicz, A Dickhout, M Murina, YM Roka-Moiia, L Malinova, C Diaz-Canestro, T Vigliarolo, S Cuzzocrea, A Szantai, B Medic, S Cassambai, A Korda, C R Revnic, G Borile, A Diokmetzidou, L Murfitt, A Budko, A Fiordelisi, DPM De Wijs-Meijler, AB Gevaert, A Noriega De La Colina, J Benes, GSB Guillermo Solache Berrocal, V Gafarov, VM Zhebel, R Prakaschandra, E L Stepien, LE Smith, MA Carluccio, Y Timasheva, M Paci, N A Dorofeyeva, CH Chimed, TI Petelina, O Sorop, A Genis, I R Parepa, M Tscharre, MV Krestjyaninov, C Maia-Rocha, L Borges, M L Sasonko, SS Kapel, K Stam, E Sommariva, S Stojkovic, J O'reilly, G Chiva-Blanch, L Malinova, A Evtushenko, J Skopal, N Sunderland, T Gegenava, MA Charnaia, N Di Lascio, SJ Tarvainen, S Malandraki-Miller, A Uitterdijk, P Benzoni, E Ruivo, E J Humphrey, MC Arokiaraj, D Franco, V Garcia-Lopez, A Aranega, C Lopez-Sanchez, D Franco, V Garcia-Lopez, A Aranega, V Garcia-Martinez, S Tayel, H Khader, N El-Helbawy, S Tayel, A Alrefai, H El-Barbary, JR Wu, ZK Dai, JL Yeh, C Sanjurjo-Rodriguez, Y Richaud-Patin, FJ Blanco, L Badimon, A Raya, Paul A Cahill, F Diomede, I Merciaro, O Trubiani, H Nahapetyan, A Swiader, J Faccini, P Boya, M Elbaz, F Zeni, I Burba, M Bertolotti, MC Capogrossi, G Pompilio, A Raucci, R Widmer-Teske, J Dutzmann, J Bauersachs, K Donde, JM Daniel, DG Sedding, N Simionescu, GM Sanda, MG Carnuta, CS Stancu, AC Popescu, MR Popescu, A Vlad, DR Dimulescu, AV Sima, E Scoditti, M Pellegrino, N Calabriso, MA Carluccio, C Storelli, R De Caterina, KS Solodenkova, EV Kalinina, MN Usachiova, J Lappalainen, M DE C Lee-Rueckert, PT Kovanen, PS Biesbroek, RWE Emmens, AC Van Rossum, LJM Juffermans, JWM Niessen, PAJ Krijnen, A Kremzer, T Samura, T Berezina, E Gronenko, MK Kim, HJ Park, SK Bae, D Sorriento, M Ciccarelli, E Vernieri, P Campiglia, B Trimarco, G Iaccarino, K E Hemmings, K E Porter, J F Ainscough, M J Drinkhill, N A Turner, HG Hiis, MV Cosson, FO Levy, T Wieland, C Macquart, M Chatzifrangkeskou, A Evans, G Bonne, A Muchir, E Kemp, M Avkiran, F Carlomosti, M D'agostino, S Beji, G Zaccagnini, B Maimone, V Di Stefano, F De Santa, S Cordisco, A Antonini, R Ciarapica, E Dellambra, F Martelli, D Avitabile, MC Capogrossi, MG Scioli, A Bielli, S Agostinelli, C Tarquini, V Tarallo, S De Falco, A Zaninoni, S Fiorelli, P Bianchi, G Teruzzi, I Squellerio, L Turnu, A Lualdi, E Tremoli, V Cavalca, YJ Lee, ES Ju, JO Choi, GY Lee, BK Lim, MANOJ Manickam, S-H Jung, S Omiya, K Otsu, C Deffge, S Nowak, M Wagner, RC Braun-Dullaeus, S Kostin, JM Daniel, A Francke, S Subramaniam, SM Kanse, K Al-Lamee, CJ Schofield, S Egginton, AH Gershlick, D Kloska, A Kopacz, A Augustyniak, J Dulak, A Jozkowicz, J Hytonen, P Halonen, J Taavitsainen, S Tarvainen, T Hiltunen, T Liimatainen, K Kalliokoski, J Knuuti, S Yla-Herttuala, M Wagner, S Weinert, B Isermann, J Lee, RC Braun-Dullaeus, J Herold, A Cochrane, S Kelaini, J Bojdo, M Vila Gonzalez, Y Hu, D Grieve, A W Stitt, L Zeng, Q Xu, A Margariti, B Reglin, W Xiang, B Nitzsche, M Maibier, AR Pries, KR Vrijsen, SAJ Chamuleau, V Verhage, CHG Metz, K Lodder, ECM Van Eeuwijk, SM Van Dommelen, PA Doevendans, AM Smits, MJ Goumans, JPG Sluijter, D Sorriento, M Bova, S Loffredo, B Trimarco, G Iaccarino, M Ciccarelli, S Appleby, N Morrell, M Baranowska-Kuczko, M Kloza, E Ambrozewicz, M Kozlowski, B Malinowska, H Kozlowska, M Monti, E Terzuoli, M Ziche, AM Mahmoud, AM Jones, JA Wilkinson, M Romero, J Duarte, MY Alexander, A Kremzer, T Berezina, E Gronenko, G Faggian, AA Kostareva, AB Malashicheva, TM Leurgans, TN Nguyen, A Irmukhamedov, LP Riber, R Mcgeogh, S Comer, A Blanco Fernandez, A Ghigo, R Blaise, NF Smirnova, N Malet, P Vincent, I Limon, S Gayral, E Hirsch, M Laffargue, V Mehta, I Zachary, I Aidonidis, K Kramkowski, W Miltyk, P Kolodziejczyk, A Gradzka, J Szemraj, E Chabielska, I Dijkgraaf, N Bitsch, S Van Hoof, F Verhaegen, R Koenen, TM Hackeng, D I Roshchupkin, K V Buravleva, VI Sergienko, DD Zhernossekov, VM Rybachuk, TV Grinenko, N Furman, P Dolotovskaya, M Shamyunov, T Denisova, M Reiner, A Akhmedov, S Keller, M Miranda, S Briand, L Barile, G Kullak-Ublick, T Luscher, G Camici, L Guida, M Magnone, P Ameri, E Lazzarini, C Fresia, S Bruzzone, E Zocchi, R Di Paola, M Cordaro, R Crupi, R Siracusa, M Campolo, G Bruschetta, R Fusco, P Pugliatti, E Esposito, J Paloczi, E Ruivo, R Gaspar, A Dinnyes, J Kobolak, P Ferdinandy, A Gorbe, Z Todorovic, D Krstic, K Savic Vujovic, D Jovicic, G Basta Jovanovic, S Radojevic Skodric, M Prostran, S Dean, CJ Mee, KL Harvey, A Hussain, C Pena, B Paltineanu, S Voinea, F Revnic, C Ginghina, T Zaglia, P Ceriotti, A Campo, P Carullo, A Armani, R Coppini, V Vida, I Olivotto, G Stellin, R Rizzuto, D De Stefani, M Sandri, D Catalucci, M Mongillo, E Soumaka, I Kloukina, M Tsikitis, M Makridakis, A Varela, C Davos, A Vlachou, Y Capetanaki, MM Iqbal, H Bennett, B Davenport, C Pinali, G Cooper, E Cartwright, A Kitmitto, NA Strutynska, LA Mys, VF Sagach, A Franco, D Sorriento, B Trimarco, G Iaccarino, M Ciccarelli, A Verzijl, K Stam, R Van Duin, IKM Reiss, DJ Duncker, D Merkus, H Shakeri, M Orije, AJ Leloup, CE Van Hove, EM Van Craenenbroeck, GRY De Meyer, CJ Vrints, K Lemmens, L Desjardins-Creapeau, R Wu, M Lamarre-Cliche, P Larochelle, L Bherer, H Girouard, M Melenovsky, A Kvasilova, J Benes, K Ruskova, D Sedmera, ABV Ana Barral, M Martin Fernandez, PRG Pablo Roman Garcia, JCLL Juan Carlos Llosa, MND Manuel Naves Diaz, CM Cesar Moris, JBCA Jorge B Cannata-Andia, IR Isabel Rodriguez, M Voevoda, E Gromova, V Maximov, D Panov, I Gagulin, A Gafarova, H Palahniuk, IP Pashkova, NV Zhebel, OL Starzhynska, DP Naidoo, K Rawojc, F J Enguita, G Grudzien, SJ Cordwell, MY White, M Massaro, E Scoditti, N Calabriso, M Pellegrino, R Martinelli, V Gatta, R De Caterina, TR Nasibullin, VV Erdman, IA Tuktarova, OE Mustafina, J Hyttinen, S Severi, GG Vorobyov, VF Sagach, KH Batmyagmar, Z Lkhagvasuren, LI Gapon, NA Musikhina, KS Avdeeva, SM Dyachkov, I Heinonen, M Van Kranenburg, VJ De Beer, Y Octavia, RJ Van Geuns, AH Van Den Meiracker, J Van Der Velden, D Merkus, DJ Duncker, FP Everson, T Ogundipe, T Grandjean, P De Boever, N Goswami, H Strijdom, A I Suceveanu, A P Suceveanu, L Mazilu, D E Tofoleanu, D Catrinoiu, M Rohla, C Hauser, K Huber, H Wojta, TW Weiss, MA Melnikova, NV Olezov, RH Gimaev, H Khalaf, VI Ruzov, R Adao, P Mendes-Ferreira, D Santos-Ribeiro, M Rademaker, AF Leite-Moreira, C Bras-Silva, LAA Alvarenga, RSP Falcao, RR Dias, S Lacchini, PS Gutierrez, JB Michel, YU I Gurfinkel, O YU Atkov, M Teichert, C Korn, C Mogler, S Hertel, C Arnold, T Korff, HG Augustin, RWB Van Duin, DPM De Wijs-Meijler, A Verzijl, DJ Duncker, D Merkus, Y D'alessandra, F M Farina, M Casella, V Catto, C Carbucicchio, A Dello Russso, I Stadiotti, S Brambilla, M Chiesa, M Giacca, G I Colombo, G Pompilio, C Tondo, F Ahlin, T Andric, D Tihanyi, J Wojta, K Huber, E O'connell, A Butt, L Murphy, S Pennington, M Ledwidge, K Mcdonald, J Baugh, C Watson, R Suades, J Crespo, R Estruch, L Badimon, A Dyachenko, V Ryabukho, V Evtushenko, YU Saushkina, YU Lishmanov, K Smyshlyaev, A Bykov, S Popov, E Pavlyukova, Y Anfinogenova, E Szigetfu, B Kapornai, E Forizs, ZS Jenei, Z Nagy, B Merkely, E Zima, A Cai, R Dworakowski, T Gibbs, S Piper, N Jegard, T Mcdonagh, M Gegenava, II Dementieva, YUA Morozov, C Barsanti, F Stea, F Lenzarini, C Kusmic, F Faita, PJ Halonen, PH Puhakka, JP Hytonen, JM Taavitsainen, S Yla-Herttuala, E A Supit, C A Carr, BCW Groenendijk, C Gorsse-Bakker, A Panasewicz, S Sneep, D Tempel, WJ Van Der Giessen, DJ Duncker, J Rys, C Daraio, P Dell'era, J Paloczi, J Pigler, A Eder, P Ferdinandy, T Eschenhagen, A Gorbe, M M Mazo, N Amdursky, N S Peters, M M Stevens, C M Terracciano
Journal: Cardiovascular Research (2016)

Introduction:We have previously demonstrated the ability of retinoic acid to regulate the expression the atrial specific markers AMHC-1 and Tbx5 during early cardiac chamber specification.However, the molecular mechanisms responsible for this process still remain unclear.At present, microRNAs represent a novel layer of complexity in the regulatory networks controlling gene expression during cardiovascular development.Purpose: The aim of this work is to study the intrinsic mechanism involved in this signaling pathway, since miR-133 has demonstrated to be involved in other earliest cardiac developmental processes.Methods: Our model is focused on developing chick at gastrula stages by in vitro electroporation of miR-133, a microRNA which is has been shown expressed at the level of linear cardiac tube.Results: Our results show the miR-133 expression at the level of the primitive heart tube.Moreover, or work reveals that overexpression of miR-133 suppresses AMHC-1 and Tbx5 expression.Conclusion: These data support that miR-133, a putative microRNA that targets RARB 3 ´UTR, regulates the early cardiac chamber specification via retinoic acid pathway.