tbuckley/cabot-search huggingface dataset:
Characterization of the intrahepatic immune response of virally-suppressed chronic hepatitis B patients to treatment with the oral TLR7 agonist GS-9620
Authors: L. Boeijen, G.W. van Oord, J. Hou, M.J. van Campenhout, M. van der Heide-Mulder, A. Gaggar, L. Li, S.P. Fletcher, R.J. de Knegt, A. Boonstra
Journal: Journal of Hepatology (2017)
HN1 contributes to migration, invasion, and tumorigenesis of breast cancer by enhancing MYC activity
Authors: Chen Zhang, Bingfei Xu, Shi Lu, Ying Zhao, Pian Liu
Journal: Molecular Cancer (2017)

Hematological and neurological expressed 1 (HN1) is upregulated in many tumors, but the role of HN1 in breast cancer progression and its regulatory mechanism have not been well understood.To study the role of HN1 in the initiation and progression of breast cancer, we examined HN1 levels in breast cancer cells and tissues and analyzed the relationship between HN1 levels and patient survival. We used mammosphere formation assay, side population analysis, wound healing assay, transwell assay, soft agar formation assay, and xenografted tumor model to determine the effect of HN1 on the expansion of breast cancer stem cells, and the migration, invasion and tumorigenesis of breast cancer. To determine whether HN1 regulates MYC, we used quantitative real-time PCR and Western blot analysis to assess the expression of MYC and their targeted genes to determine the phenotype caused by knockdown of MYC in breast cancer cell with HN1 overexpression.In this study, we found that HN1 was upregulated in breast cancer tissues. Patients with high levels of HN1 expression had significantly shorter survival than those with low HN1 expression. In breast cancer cell line, ectopic overexpression of HN1 not only promoted the expansion of breast cancer stem cells, but also promoted cell migration, invasion, and tumorigenesis, while knockdown of HN1 reduced these effects. Furthermore, there was a positive correlation between MYC (also known as c-MYC) level and HN1 level, mechanism analysis suggested HN1 promoted the expression of MYC and its targeted genes like CDK4, CCND1, p21, CAV1, and SFRP1. Downregulation of MYC abrogated the effect of HN1 overexpression in breast cancer cell lines.Taken together, these data reveal that HN1 promotes the progression of breast cancer by upregulating MYC expression, and might be a therapeutic target for breast cancer.
NGM282, a novel variant of FGF19, significantly reduces hepatic steatosis and key biomarkers of NASH: results of a Phase 2, multicenter, randomized, double-blinded, placebo controlled trial in biopsy-confirmed NASH patients
Authors: S.A. Harrison, M.F. Abdelmalek, J.F. Trotter, A.H. Paredes, H.L. Arnold, M. Kugelmas, M.R. Bashir, L. Ling, S.J. Rossi, A.M. DePaoli, M.E. Rinella, R.S. Loomba
Journal: Journal of Hepatology (2017)
Abstract P012: KDR Expression Enhances Commitment Toward the Cardiac Lineage of hESC- and iPS-Derived Cd15 <sup>+</sup> Progenitors
Authors: Elisa Di Pasquale, Portararo Paola, Roberto Rizzi, Monica Mancino, Gianluigi Condorelli
Journal: Circulation Research (2011)

Cell-based therapies represent exciting therapeutic options for myocardial regeneration after ischemic injury. Although many cell types have been proposed as a source of cardiac myocytes, the type of progenitor as well as the optimal conditions for their stimulation still represent major challenges. Moreover, molecular characterization of cardiac progenitors (CP) in vitro is still unsatisfactory: the identification of novel surface markers for their selective isolation is of utmost importance. In our study we employed different human ESCs and induced pluripotent stem (iPS) cells lines to set up a simple, reliable and efficient protocol to selectively induce cardiac fate in vitro. At first, we comparatively applied different methods and monitored the differentiation process by FACS analysis, RT-PCR and immunofluorescence for early and late markers. Our results show that sequential treatments with ActivinA and BMP4 or BMP2, ascorbic acid and TGFβ1 in a specific media formulation induce CD15 + cardiac progenitor population with an enhanced cell vitality compared to other methods. While we confirmed that CD15 is among the earliest markers upregulated after differentiation, the definition of other associated markers could better specify progenitors committed toward cardiomyogenesis. For this purpose, we systematically evaluated induction of other markers already shown associated with cardiac differentiation or heart development, that is PDGFRα, KDR, Sca1, CXCR4, cKIT. Our result demonstrate that a specific population expressing both KDR and CD15 surface markers is more committed toward cardiovascular lineages. The induced KDR + /CD15 + cell population exhibits higher levels of Gata4 and Isl1 expression (2-fold induction), compared to the KDR − /CD15 + counterpart and, after isolation, gives rise to 66.15% Troponin I positive cells (43 out of 65 in a single representative experiment), against the 26.5% (18 out of 68) detectable in the KDR − /CD15 + population. In conclusion, our studies, though preliminary, strongly indicate that KDR in CD15 + progenitor cells is an earlier marker of cardiomyogenesis.
The effect of anthtocyanins from fruits and berries on colorectal carcinogenesis in the ApcMin mouse
Authors: Darren N. Cooke, Peter Winterhalter, Michael Schwarz, William P. Steward, Andreas J. Gescher, Timothy H. Marczylo
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

2268 

Consumption of soft fruit and berries has been associated with a decrease in the incidence of colorectal cancer. The most abundant polyphenol constituents in fruits and berries are the anthocyanins, which are used as colourings in foodstuffs. Several reports suggest that anthocyanin-rich fruit or vegetable extracts prevent carcinogen-induced colorectal malignancies in rats and reduce numbers of caecal adenomas in the ApcMin (multiple intestinal neoplasia) mouse, a model of human familial adenomatous polyposis. Anthocyanins and their aglycons (anthocyanidins) are effective anti-oxidants, they also inhibit oncogenic pathways, including those mediated by epidermal growth factor receptor tyrosine-kinase (EGFR-TK) and phosphodiesterase IV (PDE) signalling. In a preliminary investigation we demonstrated a significant decrease (p < 0.05) in adenoma number when ApcMin mice (12 per group) were treated with a standardised bilberry extract \[MirtoselectR, Indena\] (Mirto)], a mixture of 15 anthocyanins. Mirto at a dose of 0.1% (w/w) in the diet reduced adenoma numbers from 62±16 in the controls to 42±10 (=67% of control). In follow-up studies the dose-dependency of this efficacy was investigated. Mice (16 per group) received AIN 95 diet containing Mirto or pure cyanidin-3-glucoside (C3G) at 0.03, 0.1 or 0.3% (w/w) with respect to anthocyanins (dose = 1, 3 or 9 mg anthocyanin/ mouse/day, respectively) for 12 weeks after weaning. For control mice or mice on Mirto at 0.03, 0.1 and 0.3% in the diet, numbers of intestinal adenomas were 62±23, 56±19 (=90% of control), 47±9 (=76%) and 40±14 (=65%), respectively. Similarly, mice receiving C3G at these doses had 48±14 (=92%), 42±16 (=81%) and 27±10 (=64%) adenomas, respectively, whilst numbers in control mice were 52±11. The difference in adenoma numbers between intervention and control was significant in the 0.3% dose group (p < 0.01). Analysis by HPLC-mass spectrometry of gastrointestinal mucosa and urine from mice on anthocyanins suggests the presence of parent anthocyanins and metabolites at measurable levels. Mirto generated methylated congeners (malvidin and peonidin glycosides) in the mucosa. Urine of mice on C3G contained also C3G-glucuronide, methylated C3G and methylated C3G glucuronide. The results suggest for the first time that dietary anthocyanins reduce intestinal adenoma development in ApcMin mice in a dose-dependent manner. Anthocyanins are worthy of further exploration of their potential as colorectal cancer chemopreventive agents.
Evidence for Quantitative and Functional Immune Deviation in Pediatric Patients with Sickle Cell Disease
Authors: Leslie Kean, Sharon Sen, Martha Ann Felder, VIN Tangpricha, Olufolake Adisa, Angelica JAMES-Herry, Iris Buchanan, Thomas Ziegler, Jessica Alvarez, Jonathan Beus, Diana Worthington-White, Jennifer Robertson, Jackie George, Joshua Cetron, Solomon Fiifi Ofori-Acquah, Ifeyinwa Osunkwo
Journal: Blood (2011)
Effect of terlipressin treatment in liver transplant-eligible patients with hepatorenal syndrome
Authors: M. Nguyen-Tat, M.-T. Häring, T. Zimmermann, M. Nagel, J. Weinmann-Menke, J.W. Rey, M.A. Wörns, P.R. Galle, J.U. Marquardt
Journal: Journal of Hepatology (2017)
Acoustic radiation force impulse elastography combined with ultrasound spleen platelets portal vein doppler score in variceal screening: improved performance compared with baveno VI criteria for avoidance of endoscopy in cirrhotics
Authors: D.I. Sherman, M. Bashyam, P. Lung, A. Sharif, P. Bassett, P. Shorvon
Journal: Journal of Hepatology (2017)
3D full-field quantification of cell-induced large deformations in fibrillar biomaterials by combining non-rigid image registration with label-free second harmonic generation
Authors: Alvaro Jorge-Peñas, Hannelore Bové, Kathleen Sanen, Marie-Mo Vaeyens, Christian Steuwe, Maarten Roeffaers, Marcel Ameloot, Hans Van Oosterwyck
Journal: Biomaterials (2017)
Incidence of and predictors for direct acting antiviral treatment failure among 3909 hepatitis C genotype 1 infected adults: real world outcomes from HCV TARGET
Authors: M.S. Sulkowski, K.R. Reddy, D.R. Nelson, J.K. Lim, J.S. Galati, A.A. Kuo, N. Terrault, A.S. Lok, B.L. Pearlman, M. Vainorius, L. Akushevich, M.W. Fried, A.M. Di Bisceglie
Journal: Journal of Hepatology (2017)
Air Pollution from Livestock Farms Is Associated with Airway Obstruction in Neighboring Residents
Authors: Floor Borlée, C. Joris Yzermans, Bernadette Aalders, Jos Rooijackers, Esmeralda Krop, Catharina B. M. Maassen, François Schellevis, Bert Brunekreef, Dick Heederik, Lidwien A. M. Smit
Journal: American Journal of Respiratory and Critical Care Medicine (2017)

Livestock farm emissions may not only affect respiratory health of farmers but also of neighboring residents.To explore associations between spatial and temporal variation in pollutant emissions from livestock farms and lung function in a general, nonfarming, rural population in the Netherlands.We conducted a cross-sectional study in 2,308 adults (age, 20-72 yr). A pulmonary function test was performed measuring prebronchodilator and post-bronchodilator FEV1, FVC, FEV1/FVC, and maximum mid-expiratory flow (MMEF). Spatial exposure was assessed as (1) number of farms within 500 m and 1,000 m of the home, (2) distance to the nearest farm, and (3) modeled annual average fine dust emissions from farms within 500 m and 1,000 m of the home address. Temporal exposure was assessed as week-average ambient particulate matter <10 μm in diameter and ammonia (NH3) concentrations before lung function measurements. Data were analyzed with generalized additive models (smoothing).A negative association was found between the number of livestock farms within a 1,000-m buffer from the home address and MMEF, which was more pronounced in participants without atopy. No associations were found with other spatial exposure variables. Week-average particulate matter <10 μm in diameter and NH3 levels were negatively associated with FEV1, FEV1/FVC, and MMEF. In a two-pollutant model, only NH3 remained associated. A 25-μg/m3 increase in NH3 was associated with a 2.22% lower FEV1 (95% confidence interval, -3.69 to -0.74), FEV1/FVC of -1.12% (-1.96 to -0.28), and MMEF of -5.67% (-8.80 to -2.55).Spatial and temporal variation in livestock air pollution emissions are associated with lung function deficits in nonfarming residents.
PET/CT findings using 18F-labeled galactose tracer (FDGal) vs. 18F-labeled glucose tracer (FDG) in patients with hepatocellular carcinoma
Authors: K.P. Bak-Fredslund, S. Kramer, G.E. Villadsen, S. Keiding, M. Sørensen
Journal: Journal of Hepatology (2017)
The anti-inflammatory receptor TREM-2 protects the liver from hepatocellular carcinoma development
Authors: A. Esparza-Baquer, I. Labiano, O. Erice, O. Sharif, F. Oakley, A. Lapitz, P. Olaizola, P.Y. Lee-Law, E. Hijona, R. Jiménez-Agüero, S. Knapp, D.A. Mann, L. Bujanda, J.M. Banales, M.J. Perugorria
Journal: Journal of Hepatology (2017)
Evaluation of ciclopirox efficacy in rhabdomyosarcoma.
Authors: Alaa Altahan, Jun Yang, Christopher L. Morton, Andrew M. Davidoff
Journal: Journal of Clinical Oncology (2014)

10059 Background: Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in the pediatric age group. Despite current multimodal treatment, a significant portion of patients still have poor outcomes. Recent studies have shown that ciclopirox (CPX), an antifungal agent, has antitumor activity in some types of cancers like leukemia and breast cancer. The aim of this study is to evaluate the therapeutic efficacy of ciclopirox in treating rhabdomyosarcoma both in-vitro and in-vivo. Methods: The effect of ciclopirox on RMS in-vitro was evaluated by performing dose and time response curves in 4 RMS cell lines (Rh30, Rh41 alveolar and Rh18, RD embryonal). Flow cytometry was used to evaluate cell cycle status and degree of apoptosis. Western blotting was used to evaluate protein changes. Levels of N-MYC and C-MYC in RMS were analyzed using R2: microarray analysis and visualization platform (http://r2.amc.nl). The efficacy of CPX in-vivowas tested in a subcutaneous RMS xenograft model in SCID mice. Once tumors were established, mice were randomized to treatment or control groups. Results: In-vitro studies showed high sensitivity of all the RMS cell lines to CPX with IC50 = 0.5-2.5uM. In contrast, normal human fibroblasts were minimally affected in this dose range. Cell cycle and apoptosis analysis showed different responses to CPX in different cell lines. Interestingly, western blot analysis showed a significant reduction of N-MYC and C-MYC levels with CPX treatment. Analysis of public data sets showed increased expression of N-MYC and C-MYC in RMS compared to normal muscle. In-vivoresults showed that CPX treatment leads to significant delay in tumor growth, with 47% reduction in tumor volume after 26 days of starting treatment (p-value 0.005), compared to the control group. Conclusions: Our results show that ciclopirox is effective at inhibiting rhabdomyosarcoma cell proliferation. Interestingly, we found that CPX significantly reduces N-MYC and C-MYC expression. Further studies are being done to better understand the mechanism behind this change. Finally, in-vivo results demonstrated therapeutic efficacy of CPX in treating RMS tumors. Results of our study suggest a potential therapeutic role for ciclopirox in the treatment of rhabdomyosarcoma.
Primary Testicular Lymphoma:a Single Center Experience and Literature Review
Authors: Ling Li, Fangwen Zhang, Ken H. Young, Wenjing Duan, Zhaoming Li, Jiaqin Yan, Xiaorui Fu, Lei Zhang, Xin Li, Zhenchang Sun, Xudong Zhang, Yu Chang, Feifei Nan, Mingzhi Zhang
Journal: Blood (2016)
Sex- and Age-Adjusted Population Analysis of Prevalence Estimates for Hidradenitis Suppurativa in the United States
Authors: Amit Garg, Joslyn S. Kirby, Jonathan Lavian, Gloria Lin, Andrew Strunk
Journal: JAMA Dermatology (2017)

The true prevalence of hidradenitis suppurativa (HS) is unknown.To establish standardized overall and group-specific prevalence estimates for HS in the United States.This retrospective analysis included a demographically heterogeneous population-based sample of more than 48 million unique patients across all US census regions. As of October 27, 2016, a total of 47 690 patients with HS were identified using electronic health record data.Standardized overall point prevalence for HS and sex-, age-, and race-specific prevalence estimates of HS in the general US population.Of the 47 690 patients with HS (26.2% men and 73.8% women), the overall HS prevalence in the US population sample was 0.10%, or 98 per 100 000 persons (95% CI, 97-99 per 100 000 persons). The adjusted prevalence in women was 137 per 100 000 (95% CI, 136-139 per 100 000), more than twice that of men (58 per 100 000; 95% CI, 57-59 per 100 000; P < .001). The prevalence of HS was highest among patients aged 30 to 39 years (172 per 100 000; 95% CI, 169-275 per 100 000) compared with all other age groups (range, 15-150 per 100 000; P < .001). Adjusted HS prevalences among African American (296 per 100 000; 95% CI, 291-300 per 100 000) and biracial (218 per 100 000; 95% CI, 202-235 per 100 000) patients were more than 3-fold and 2-fold greater, respectively, than that among white patients (95 per 100 000; 95% CI, 94-96 per 100 000; P < .001).Hidradenitis suppurativa is an uncommon, but not rare, disease in the United States that disproportionately affects female patients, young adults, and African American and biracial patients.
Simultaneous non-invasive assessment of liver injury and body composition in alpha1-antitrypsin deficiency
Authors: A. Arslanow, M.C. Reichert, S. Gatter, S. Fähndrich, R. Bals, K. Hamesch, P. Strnad, C. Trautwein, F. Lammert
Journal: Journal of Hepatology (2017)
Understanding the Emergence of Chronic Posttraumatic Stress Disorder Through Acute Stress Symptom Networks
Authors: Christina Haag, Donald J. Robinaugh, Anke Ehlers, Birgit Kleim
Journal: JAMA Psychiatry (2017)

To accelerate recovery after traumatic events and prevent posttraumatic stress disorder (PTSD), it is critical to understand the dynamic interplay of acute stress symptoms and how they develop over time into chronic PTSD.Bryant et al 1 examined relationships among PTSD symptoms during the acute posttrauma period using network analyses.They concluded that symptoms highly central to the acute symptom network, such as intrusive trauma memories, may be especially important to the development of chronic PTSD.We tested this prediction by examining whether acute symptoms identified as highly central by Bryant et al 1 were stronger predictors of subsequent PTSD than were low centrality symptoms in an independent sample of assault survivors.We then extended the findings by examining paths by which acute symptoms may relate to chronic PTSD.Methods | We analyzed data from 171 assault survivors, 2 who completed a self-report assessment of acute symptoms at 2
Abstract IA05: Autophagy prevents fatal nucleotide pool depletion in Ras-driven cancer cells
Authors: Eileen White
Journal: Clinical Cancer Research (2017)

Abstract Autophagy degrades and recycles proteins and organelles essential for quality control and survival in starvation. In genetically engineered mouse models (GEMMs) for Kras-driven lung cancer, autophagy prevents the accumulation of defective mitochondria and promotes tumor growth and malignancy. Autophagy-deficient tumor-derived cell lines (TDCLs) are respiration impaired, starvation sensitive, and glutamine-dependent. To survive starvation, autophagy may eliminate defective mitochondria or supply mitochondrial substrates. Here, we sequenced the mitochondrial genome from Kras-driven lung tumors and found higher mutant allele frequencies without autophagy-related-7 (Atg7). But the low pathogenic allele frequency was insufficient to account for metabolic defects. In starvation, Atg7 deficiency attenuated substrate supply for mitochondrial oxygen consumption and increased nucleotide degradation. The resulting nucleotide pool depletion and energy crisis in starvation was rescued by nucleoside supplementation. Thus the role for autophagy in Kras-driven tumor cells is to provide substrates to mitochondria to maintain nucleotide pools and energy homeostasis, which promotes survival to nutrient limitation. Citation Format: Eileen White. Autophagy prevents fatal nucleotide pool depletion in Ras-driven cancer cells. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Targeting the Vulnerabilities of Cancer; May 16-19, 2016; Miami, FL. Philadelphia (PA): AACR; Clin Cancer Res 2017;23(1_Suppl):Abstract nr IA05.
Maintenance chemotherapy with pemetrexed in epiteliomorfe malignant pleural mesothelioma.
Authors: Angelo Nacci, Claudio Lotesoriere, Michele Montrone, Salvatore Pisconti, Laura Orlando, Palma Fedele, Pietro Rizzo, Nicola Calvani, Enrica Mazzoni, Margherita Cinefra, Antonella Marino, Francesco Sponziello, Saverio Cinieri
Journal: Journal of Clinical Oncology (2013)

e18545 Background: Maintenance chemotherapy with pemetrexed is not the standard treatment of choice in patients with locally advanced or metastatic epiteliomorfe malignant pleural mesothelioma (EMPM). We would assess the safety and efficacy of a treatment with pemetrexed until progression disease after 4 or 6 cycles of induction therapy with or without platin. Methods: From July 2008 to September 2012, 21 patients (18 males and 3 females with a median age of 67 years range 58-84) with locally advanced or metastatic epiteliomorfe malignant pleural mesothelioma (EMPM) were enrolled. In all patients histology was epiteliomorfe malignant mesothelioma. Only 15 patients (71,4%) had a PS 0 whereas 6 (28,6%) had a PS 1. All patients received an induction therapy with or without platin. Each patient received an average of 5,6 cycles of induction chemotherapy. Then all patients received a maintenance chemotherapy with pemetrexed 500 mg/mq intavenously over 10 minutes every 3 weeks. Each patient received an average of 7,3 cycles of maintenance chemotherapy. All patients received folic acid and vitamin B12 supplementation to improve safety. Results: At the time of analysis all patients were evaluable for response. Fourteen patients (66,6 %) had a partial response and two of these underwent surgery and obtained a complete response. Six patients (28,5%) had a stable disease. The median overall survival was 13 months, while median progression-free survival was 11 months. Grade 2-3 of WHO haematological toxicities (anemia and neutropenia) occurred in 4 patient (19%). We also observed grade 2-3 of WHO gastrointestinal toxicities (diarrhea, nausea and vomiting) in 2 patient (9.5%). Grade 2 of lack of appetite and asthenia occurred in 3 patients (14.3%). Conclusions: Our data show that a maintenance chemotherapy with pemetrexed in EMPM resulted in a moderate overall survival (13 months). These results indicate that patients with EMPM could benefit from a maintenance treatment with pemetrexed.
In vivo pharmacodynamics of GS-5801, a liver targeted prodrug of a lysine demethylase 5 inhibitor with antiviral activity against hepatitis B virus
Authors: S.A. Gilmore, C.A. Snyder, R. Dick, M. Matles, D. Tam, C.H. Tay, J. Farand, E. Paoli, W.E. Delaney, B. Feierbach, G.T. Notte
Journal: Journal of Hepatology (2017)
Setting Research Priorities for Kidney Cancer
Authors: Jennifer M. Jones, Jaimin Bhatt, Jonathan Avery, Andreas Laupacis, Katherine Cowan, Naveen S. Basappa, Joan Basiuk, Christina Canil, Sohaib Al-Asaaed, Daniel Y.C. Heng, Lori Wood, Dawn Stacey, Christian Kollmannsberger, Michael A.S. Jewett
Journal: European Urology (2017)