tbuckley/cabot-search huggingface dataset:
Multiplex molecular profiling of breast cancer cell lines with quantum dot-antibody conjugates
Authors: Maksym Yezhelyev, Caroline Morris, Xiaohu Gao, Shuming Nie, Melinda Lewis, Cynthia Cohen, Ruth M. O’Regan
Journal: Cancer Research (2005)

2173  Identification of biomarkers in breast tumors is of primary importance in predicting the likely benefit of systemic therapies. Conventional biomarkers, such as estrogen receptor (ER), progesterone receptor (PR) and HER2/neu (HER2) can be used to predict the outcome from hormonal therapies and trastuzumab. Techniques currently used to detect and quantify these conventional biomarkers such as immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) are unable to perform multiplex antigen determination. Semiconductor nanoparticles such as quantum dots (QDs) have unique optical properties and can be conjugated with antibodies. QD-antibody conjugates allow the simultaneous detection of several proteins on a single specimen thereby avoiding problems with tumor heterogeneity. Methods: Antibodies conjugated to multicolor QDs were used to perform multiplex labeling of 5 breast cancer cell lines. Antibodies for HER2, ER and PR were conjugated to 565nm, 605nm and 655nm QDs (QDots Corp., CA) respectively. MCF-7, T47D, BT474, SKBR3 and MDA-MB-231 human breast cancer cell lines, and NH3T3 fibroblasts cells (used as controls) were plated in 8-wells chamber slides, fixed and stained simultaneously with anti-HER2, anti-ER and anti-PR antibody-QD conjugates. Quantitative analysis of the labeled antigens was then completed via single-cell spectroscopy using the SpectroPro 150 fluorescence microscope and a single-stage spectrograph. Results: We obtained a wavelength- resolved spectrum with three separated peaks representing expression levels of HER2, ER and PR. One hundred single cells from every cell line were analyzed and an average of intensity patterns was calculated. Estimated antigen amount is shown in the table (Fluorescent intensity -a.u.x10-2). We found an excellent correlation between biomarker expression levels using conjugated QDs and by Western blot, IHC and FISH. Conclusions: Using conjugated QDs, we have detected ER, PR and HER2 simultaneously on a single specimen. Conjugated QDs will ultimately allow the detection of up to 100 proteins simultaneously on a single specimen, thereby, opening exciting new possibilities in cancer molecular profiling using nanotechnology.
Stress Lymphoscintigraphy for Early Detection and Management of Secondary Limb Lymphedema
Authors: Girolamo Tartaglione, Giuseppe Visconti, Roberto Bartoletti, Stefano Gentileschi, Marzia Salgarello, Domenico Rubello, Patrick M. Colletti
Journal: Clinical Nuclear Medicine (2017)

Cancer treatments with axillary or pelvic lymph nodes dissection and radiation place patients at lifelong risk for the development of secondary lymphedema. Our aim was to evaluate the role of stress lymphoscintigraphy for early detection and management of secondary lymphedema.Stress lymphoscintigraphy was performed within 1 year after surgery and the completion of chemotherapy and radiation treatments. All patients were classified by the International Society of Lymphology clinical stages from 0 to 3. A dose of 50 MBq of 99mTc-HSA-nanocolloidal in 0.4mL was injected intradermally at the first and fourth intermetacarpal spaces on the hand, for the upper limb with edema, or at the first intermetatarsal space and at the lateral malleolus for lower extremities. Two planar static scans at rest were acquired immediately after tracer injection. Stress scans were acquired after weight lifting for upper extremity or stepping for 2 minutes for lower-extremity edema. After that, the patients underwent prolonged muscular exercise limited by symptoms, and later scans were acquired at 60 minutes to visualize regional lymph nodes and the effects of sustained muscular exercise. Transport Index was evaluated.Five patterns of lymphoscintigraphy were observed. In our experience, patients with types I to III pattern benefit from an exercise program as a first-line treatment. Patterns IVand V seem to be predictive of lymphedema.The abnormal patterns found may provide the basis for earlier complex physical therapy or microsurgical treatment of lymphatic disorders in patients resulting in improved outcomes.
Age at Diagnosis as a Relative Contraindication for Intervention in Facial Nonmelanoma Skin Cancer
Authors: Ruvi Chauhan, Brook N. Munger, Michael W. Chu, Imtiaz Munshi, Adam C. Cohen, William A. Wooden, Sunil S. Tholpady
Journal: JAMA Surgery (2017)

variability in the multidisciplinary management of OSTRICH member institutions as well as poor projected compliance with anticipated NAPRC accreditation standards.As a result, considerable opportunity is available for improvements in multidisciplinary treatment standardization.
Does Cognitive Training Prevent Cognitive Decline?
Authors: Mary Butler, Ellen McCreedy, Victoria A. Nelson, Priyanka Desai, Edward Ratner, Howard A. Fink, Laura S. Hemmy, J. Riley McCarten, Terry R. Barclay, Michelle Brasure, Heather Davila, Robert L. Kane
Journal: Annals of Internal Medicine (2017)

Background: Structured activities to stimulate brain function—that is, cognitive training exercises—are promoted to slow or prevent cognitive decline, including dementia, but their effectiveness is highly debated. Purpose: To summarize evidence on the effects of cognitive training on cognitive performance and incident dementia outcomes for adults with normal cognition or mild cognitive impairment (MCI). Data Sources: Ovid MEDLINE, EMBASE, the Cochrane Central Register of Controlled Trials, and PsycINFO through July 2017, supplemented by hand-searches. Study Selection: Trials (published in English) lasting at least 6 months that compared cognitive training with usual care, waitlist, information, or attention controls in adults without dementia. Data Extraction: Single-reviewer extraction of study characteristics confirmed by a second reviewer; dual-reviewer risk-of-bias assessment; consensus determination of strength of evidence. Only studies with low or medium risk of bias were analyzed. Data Synthesis: Of 11 trials with low or medium risk of bias, 6 enrolled healthy adults with normal cognition and 5 enrolled adults with MCI. Trainings for healthy older adults were mostly computer based; those for adults with MCI were mostly held in group sessions. The MCI trials used attention controls more often than trials with healthy populations. For healthy older adults, training improved cognitive performance in the domain trained but not in other domains (moderate-strength evidence). Results for populations with MCI suggested no effect of training on performance (low-strength and insufficient evidence). Evidence for prevention of cognitive decline or dementia was insufficient. Adverse events were not reported. Limitation: Heterogeneous interventions and outcome measures; outcomes that mostly assessed test performance rather than global function or dementia diagnosis; potential publication bias. Conclusion: In older adults with normal cognition, training improves cognitive performance in the domain trained. Evidence regarding prevention or delay of cognitive decline or dementia is insufficient. Primary Funding Source: Agency for Healthcare Research and Quality.
Hematologic, cytogenetic and molecular response to Glivec (STI571) in Ph+ chronic myeloid leukemia (CML) in accelerated and blastic phase: a prospective study of the Italian cooperative study group on CML
Authors: G Rosti, D Alberti, A De Vivo, F Bonifazi, E Trabacchi, S Bassi, K Vecchi, N Testoni, M Amabile, E Ottaviani, M Tiribelli, E Gottardi, D Cilloni, F Pane, M Intrieri, F Salvatore, G Martinelli, G Saglio, E Montefusco, S Tura, M. Baccarani
Journal: Blood (2001)
The Western environment reduces innate immune cytokine production in Chinese immigrants
Authors: Aarti Saiganesh, Belinda J. Hales, Shu Chen, Emilija Filipovska-Naumovska, Gavin Pereira, Mathieu Garand, Tobias R. Kollmann, Andrew J. Currie, Peter N. Le Souëf, Guicheng Zhang
Journal: Journal of Allergy and Clinical Immunology (2017)
Safety of Magnetic Resonance Imaging in Patients with Cardiac Devices
Authors: Saman Nazarian, Rozann Hansford, Amir A. Rahsepar, Valeria Weltin, Diana McVeigh, Esra Gucuk Ipek, Alan Kwan, Ronald D. Berger, Hugh Calkins, Albert C. Lardo, Michael A. Kraut, Ihab R. Kamel, Stefan L. Zimmerman, Henry R. Halperin
Journal: New England Journal of Medicine (2017)

Patients who have pacemakers or defibrillators are often denied the opportunity to undergo magnetic resonance imaging (MRI) because of safety concerns, unless the devices meet certain criteria specified by the Food and Drug Administration (termed "MRI-conditional" devices).
The role of adult attachment orientation and coping in psychological adjustment to living with skin conditions
Authors: M. Krasuska, A.C. Lavda, A.R. Thompson, A. Millings
Journal: British Journal of Dermatology (2017)

Skin conditions can be associated with significant psychological distress. Investigation of attachment orientation and associated use of coping strategies is a promising perspective from which to investigate psychological adjustment to skin conditions.To examine the role of adult attachment orientation, conceptualized as two dimensions - attachment avoidance and attachment anxiety - in psychological adjustment [appearance-related distress and skin-related quality of life (QoL)], and the mediating role of two coping strategies - defeatism and activity.Attachment avoidance and attachment anxiety, use of coping strategies, appearance-related distress and skin-related QoL were assessed via a cross-sectional online survey in 207 adults with skin conditions. Multiple mediation analyses were performed using PROCESS pathway analysis.Overall, higher attachment avoidance and higher attachment anxiety were associated with higher appearance-related distress and greater impact on emotions and functioning aspects of QoL. For attachment anxiety, the relationship was fully explained by more frequent use of defeatism coping. For attachment avoidance, the relationship was partially explained by more frequent use of defeatism coping (and in the case of appearance-related distress also more frequent use of activity coping), and higher attachment avoidance was also associated with poorer psychological adjustment in a way that could not be explained by use of assessed coping strategies.Insecure attachment orientation (high attachment avoidance or high attachment anxiety) is linked to poorer adjustment. People with insecure attachment might benefit from psychological interventions that target underlying attachment orientation.
Maternal Stress and Depression are Associated with Development of a High-Wheeze, Low-Atopy Phenotype in Their Young Offspring
Authors: Sima K. Ramratnam, Agustin Calatroni, Leonard Bacharier, Daniel J. Jackson, Avraham Beigelman, Robert A. Wood, Meyer Kattan, George T. O'Connor, Cynthia M. Visness, Peter J. Gergen, James E. Gern
Journal: Journal of Allergy and Clinical Immunology (2018)
Normal interventricular differences in tissue architecture underlie right ventricular susceptibility to conduction abnormalities in a mouse model of Brugada syndrome
Authors: Allen Kelly, Simona Salerno, Adam Connolly, Martin Bishop, Flavien Charpentier, Tomas Stølen, Godfrey L Smith
Journal: Cardiovascular Research (2017)

Loss-of-function of the cardiac sodium channel NaV1.5 is a common feature of Brugada syndrome. Arrhythmias arise preferentially from the right ventricle (RV) despite equivalent NaV1.5 downregulation in the left ventricle (LV). The reasons for increased RV sensitivity to NaV1.5 loss-of-function mutations remain unclear. Because ventricular electrical activation occurs predominantly in the transmural axis, we compare RV and LV transmural electrophysiology to determine the underlying cause of the asymmetrical conduction abnormalities in Scn5a haploinsufficient mice (Scn5a+/−). Optical mapping and two-photon microscopy in isolated-perfused mouse hearts demonstrated equivalent depression of transmural conduction velocity (CV) in the LV and RV of Scn5a+/− vs. wild-type littermates. Only RV transmural conduction was further impaired when challenged with increased pacing frequencies. Epicardial dispersion of activation and beat-to-beat variation in activation time were increased only in the RV of Scn5a+/− hearts. Analysis of confocal and histological images revealed larger intramural clefts between cardiomyocyte layers in the RV vs. LV, independent of genotype. Acute sodium current inhibition in wild type hearts using tetrodotoxin reproduced beat-to-beat activation variability and frequency-dependent CV slowing in the RV only, with the LV unaffected. The influence of clefts on conduction was examined using a two-dimensional monodomain computational model. When peak sodium channel conductance was reduced to 50% of normal the presence of clefts between cardiomyocyte layers reproduced the activation variability and conduction phenotype observed experimentally. Normal structural heterogeneities present in the RV are responsible for increased vulnerability to conduction slowing in the presence of reduced sodium channel function. Heterogeneous conduction slowing seen in the RV will predispose to functional block and the initiation of re-entrant ventricular arrhythmias.
Validación externa temporal y mejora de los modelos predictivos disponibles de hemorragia diferida tras RME de lesiones colo-rectales de gran tamaño
Authors: E Albéniz, M Fraile, M Rullan, M Pellisé, J Merlo, CJ Gargallo, M Rodríguez-Téllez, E Redondo, P Alonso, A Álvarez
Journal: Endoscopy (2017)

La hemorragia diferida (HD) es la complicación grave más frecuente de la RME. Existen solamente dos modelos publicados al respecto (GSEED-Resección Endoscópica (G-RE) y Grupo Australiano (GA) no validados por otros grupos.
Detection of cotinine in newborn dried blood spots
Authors: Logan Spector, Stephen Hecht, Simona Ognjanovic, Steven Carmella, Julie Ross
Journal: Cancer Research (2007)

830   Evidence is growing that many childhood cancers are initiated  in utero . Although tobacco contains many carcinogens that cross the placenta, associations between maternal tobacco use during pregnancy and childhood cancer have been inconsistently observed. However, recall or social desirability biases may influence smoking data collected by maternal interview since most studies use the case-control design. Detection of tobacco biomarkers in dried blood spots (DBS), which are increasingly stored by state newborn screening programs, may improve retrospective assessment of fetal tobacco exposure. As proof-of-principle we examined the tobacco-specific biomarker cotinine in DBS of 20 infants enrolled in a pilot study of pregnancy among low-income women. Between January and June of 1999 we recruited 107 pregnant women (
Outcomes After Adjustable Gastric Banding
Authors: George Fielding
Journal: JAMA Surgery (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Bacillus spore-based oral carriers loading curcumin for the therapy of colon cancer
Authors: Liang Yin, Zhan Meng, Yuxiao Zhang, Kaikai Hu, Wuya Chen, Kaibin Han, Bao-Yan Wu, Rong You, Chu-Hua Li, Ying Jin, Yan-Qing Guan
Journal: Journal of Controlled Release (2017)
The Bidirectional Association Between Depression and Severe Hypoglycemic and Hyperglycemic Events in Type 1 Diabetes
Authors: Paola Gilsanz, Andrew J. Karter, Michal Schnaider Beeri, Charles P. Quesenberry, Rachel A. Whitmer
Journal: Diabetes Care (2017)

OBJECTIVE Severe hyperglycemia and hypoglycemia (“severe dysglycemia”) are serious complications of type 1 diabetes (T1D). Depression has been associated with severe dysglycemia in type 2 diabetes but has not been thoroughly examined specifically in T1D. We evaluated bidirectional associations between depression and severe dysglycemia among older people with T1D. RESEARCH DESIGN AND METHODS We abstracted depression and severe dysglycemia requiring emergency room visit or hospitalization from medical health records in 3,742 patients with T1D during the study period (1996–2015). Cox proportional hazards models estimated the associations between depression and severe dysglycemia in both directions, adjusting for demographics, micro- and macrovascular complications, and HbA1c. RESULTS During the study period, 41% had depression and 376 (11%) and 641 (20%) had hyperglycemia and hypoglycemia, respectively. Depression was strongly associated with a 2.5-fold increased risk of severe hyperglycemic events (hazard ratio [HR] 2.47 [95% CI 2.00, 3.05]) and 89% increased risk of severe hypoglycemic events (HR 1.89 [95% CI 1.61, 2.22]). The association was strongest within the first 6 months (HRhyperglycemia 7.14 [95% CI 5.29, 9.63]; HRhypoglycemia 5.58 [95% CI 4.46, 6.99]) to 1 year (HRhyperglycemia 5.16 [95% CI 3.88, 6.88]; HRhypoglycemia 4.05 [95% CI 3.26, 5.04]) after depression diagnosis. In models specifying severe dysglycemia as the exposure, hyperglycemic and hypoglycemic events were associated with 143% (HR 2.43 [95% CI 2.03, 2.91]) and 74% (HR 1.75 [95% CI 1.49, 2.05]) increased risk of depression, respectively. CONCLUSIONS Depression and severe dysglycemia are associated bidirectionally among patients with T1D. Depression greatly increases the risk of severe hypoglycemic and hyperglycemic events, particularly in the first 6 months to 1 year after diagnosis, and depression risk increases after severe dysglycemia episodes.
Lineage tracking of origin and fate of smooth muscle cells in atherosclerosis
Authors: Jacob F Bentzon, Mark W Majesky
Journal: Cardiovascular Research (2017)

Advances in lineage-tracking techniques have provided new insights into the origins and fates of smooth muscle cells (SMCs) in atherosclerosis. Yet new tools present new challenges for data interpretation that require careful consideration of the strengths and weaknesses of the methods employed. At the same time, discoveries in other fields have introduced new perspectives on longstanding questions about steps in atherogenesis that remain poorly understood. In this article, we address both the challenges and opportunities for a better understanding of the mechanisms by which cells appearing as or deriving from SMCs accumulate in atherosclerosis.
Baseline total kidney volume and the rate of kidney growth are associated with chronic kidney disease progression in Autosomal Dominant Polycystic Kidney Disease
Authors: Alan S.L. Yu, Chengli Shen, Douglas P. Landsittel, Peter C. Harris, Vicente E. Torres, Michal Mrug, Kyongtae T. Bae, Jared J. Grantham, Frederic F. Rahbari-Oskoui, Michael F. Flessner, William M. Bennett, Arlene B. Chapman
Journal: Kidney International (2017)
Drug shortages cost NHS £38m in November
Authors: Gareth Iacobucci
Journal: BMJ (2017)

Ongoing drug shortages cost the NHS £38m (€43m; $51m) last month as more emergency price increases were agreed to boost stocks, an analysis has shown.1

A record 91 emergency price rises were agreed in November, as the NHS continued to experience supply problems that are estimated to have cost the service more than £200m since April 2017. However, the analysis, by the Evidence-Based Medicine DataLab at the University of Oxford, showed that the total amount overpaid last month was …
Molecular markers to predict response to selective fibroblast growth factor receptor inhibitors (FGFRinh) in patients (pts) with FGFR-amplified (amp) or mutated (mut) tumors.
Authors: Cinta Hierro, Marta Serrano, Fiorella Ruiz-Pace, Juan Martin-Liberal, Maria Ochoa de Olza, Analía Azaro, Irene Brana, Maria Vieito, Maria Alsina, Jose Manuel Perez Garcia, Helena Verdaguer, Teresa Macarulla, Monica Sanchez, Violeta Serra, Paolo Nuciforo, Ana Vivancos, Elena Garralda, Rodrigo Dienstmann, Josep Tabernero, Jordi Rodon
Journal: Journal of Clinical Oncology (2017)

2581 Background: Several FGFR and ligand (11q) alterations have been described in cancer. While FGFR fusions are recognized biomarkers of response to FGFRinh, it is still unclear to what extent FGFRamp, FGFR mRNA high expression (mRNAh) or FGFRmut predict sensitivity in the clinic. Methods: Retrospective analysis of pts with molecularly-selected FGFRamp/mRNAh/mut tumors treated with FGFRinh in phase 1 trials at our institution. Mut were detected with IlluminaÒ or Foundation OneÒ. FGFR1-2amp were analyzed by in situ hybridization and mRNA levels by qRT-PCR or nCounterÒ. Clinical benefit (ClinBen) was defined as any tumor shrinkage plus disease control for ³ 4 months (m). Time to progression (TTP) was defined as time between start of FGFRinh and end for any cause. Results: From 2011 to 2016, 36 pts with FGFRamp(25)/mRNAh(5)/mut(6) received an FGFRinh (irreversible- [11 cases (c)] or reversible-FGFR1-4inh [23 c], isoform-specific FGFRinh [3 c] or combo with PI3Kinh [1 c]). Median age 55 yrs (34-76); median prior palliative lines was 3 (0-8); tumor types: breast (17), colorectal (3), esophagus (3), liver (3), lung (3), others (7). Median TTP was 1.67 m (CI 95%; 1.40-2.87). In the FGFRamp/mRNAh population (30), 7 pts achieved ClinBen (23%). 4 out of these seven pts had mRNAh (1 FGFR2amp breast with FGFR2 mRNAh, 1 bladder FGFR3 mRNAh and 2 liver FGFR4 mRNAh without known amp), and 2 pts harboured 11q co-amplification (1 FGFR2amp breast, 1 FGFR2amp head and neck). There was no correlation between ClinBen and level of FGFRamp in the overall population (p = 0.51) or in the breast cancer group (p = 0.29). Of 6 FGFRmut pts, one with bladder cancer had ClinBen (clonal oncogenic FGFR3 S249C). The remaining 5 unresponsive FGFRmut pts had subclonal events, some of these FGFRmut were of unknown functional significance, and had coexisting oncogene mut in MAPK or PI3K pathways. Conclusions: Our results suggest that ClinBen with FGFRinh in the FGFRamp setting is enriched in pts with high mRNA expression and/or ligand co-amplification, and in the FGFRmut population may be dependent on clonality and functionality of the event and co-existence of driver mut.