tbuckley/cabot-search huggingface dataset:
Abstract 18661: Regulation of Lymphatic Physiology by Connexin47
Authors: Merlijn J Meens, Issa Kutkut, Amélie Sabine, Tatiana V Petrova, Brenda R Kwak
Journal: Circulation (2015)

Introduction: Mutations in GJC2, which encodes connexin47 (Cx47), are associated with lymphedema. Moreover, Cx47 mRNA was recently found in human lymphatic endothelial cells (LECs). Hypothesis: Lymphatic endothelial Cx47 has a role in lymphatic physiology and pathology. Methods: Confocal microscopy and qPCRs were used to assess expression of Cx47. Lymphatic drainage was studied by subcutaneous Evans Blue injections in the footpad of 12 week-old ApoE -/- or Cx47 eGFP/eGFP ApoE -/- mice. Atherosclerosis was studied in 15 months-old ApoE -/- or Cx47 eGFP/eGFP ApoE -/- mice. Results: Cx47 was expressed in lymphangions of mesenteric collecting vessels in Cx47 eGFP/eGFP Prox1-mOrange2 mice as assessed by microscopy and its expression was confirmed by qPCR on mesentery, gut and skin mRNA of ApoE -/- but not Cx47 eGFP/eGFP ApoE -/- mice. Interestingly, lymphatic drainage was enhanced in Cx47-deficient mice (n=10/11, p&lt;0.05). To address whether Cx47 affects chronic immuno-inflammatory pathology atherosclerotic plaque formation was studied in 15-month old male ApoE -/- and Cx47 eGFP/eGFP ApoE -/- mice on regular chow. These studies showed that Cx47-deficiency does not affect the size or composition (e.g. lipid, collagen or macrophage content) of aortic plaques in the aortic root. However, plaque development in the thoracic-abdominal aorta of 15 months-old mice tended to be larger in Cx47 eGFP/eGFP ApoE -/- mice (21±5 vs 30±4 % lipid area, n=10, p=0.08). Unexpectedly, total cholesterol (TC) and LDL, but not HDL, triglycerides or free fatty acids, were increased in serum of Cx47 eGFP/eGFP ApoE -/- mice as compared to ApoE -/- serum (TC: 472±24 vs 647±18 mg/dL, n=10, p&lt;0.01; and LDL: 272±20 vs 443±17 mg/dL, n=10, p&lt;0.001, respectively). Increased lymphatic drainage from plaques in Cx47 eGFP/eGFP ApoE -/- mice may, partially, compensate for increased serum TC and LDL thereby preventing exuberant atherosclerotic plaque formation despite increased serum lipid levels. Conclusions: In conclusion, Cx47 is expressed in LECs of the lymphangion and seems implicated in multiple aspects of lymphatic (patho)physiology.
Arterial Pulse Pressure Variation with Mechanical Ventilation
Authors: Jean-Louis Teboul, Xavier Monnet, Denis Chemla, Frédéric Michard
Journal: American Journal of Respiratory and Critical Care Medicine (2018)

Fluid administration leads to a significant increase in cardiac output in only half of ICU patients. This has led to the concept of assessing fluid responsiveness before infusing fluid. Pulse pressure variation (PPV), which quantifies the changes in arterial pulse pressure during mechanical ventilation, is one of the dynamic variables that can predict fluid responsiveness. The underlying hypothesis is that large respiratory changes in left ventricular stroke volume, and thus pulse pressure, occur in cases of biventricular preload responsiveness. Several studies showed that PPV accurately predicts fluid responsiveness when patients are under controlled mechanical ventilation. Nevertheless, in many conditions encountered in the ICU, the interpretation of PPV is unreliable (spontaneous breathing, cardiac arrhythmias) or doubtful (low Vt). To overcome some of these limitations, researchers have proposed using simple tests such as the Vt challenge to evaluate the dynamic response of PPV. The applicability of PPV is higher in the operating room setting, where fluid strategies made on the basis of PPV improve postoperative outcomes. In medical critically ill patients, although no randomized controlled trial has compared PPV-based fluid management with standard care, the Surviving Sepsis Campaign guidelines recommend using fluid responsiveness indices, including PPV, whenever applicable. In conclusion, PPV is useful for managing fluid therapy under specific conditions where it is reliable. The kinetics of PPV during diagnostic or therapeutic tests is also helpful for fluid management.
Abstract 16798: Urinary Biomarkers at Admission in Out-of-hospital Cardiac Arrest Patients May Predict Acute Kidney Injury and Patient Outcome
Authors: Sigrid Beitland, Bård Waldum-Grevbo, Espen R Nakstad, Jens-Petter Berg, Anne-Marie S Trøseid, Berit S Brusletto, Cathrine Brunborg, Geir Ø Andersen, Kjetil Sunde
Journal: Circulation (2016)

Introduction: Urine biomarkers may be used to diagnose acute kidney injury (AKI) and predict patient outcome. Hypothesis: Urinary AKI biomarkers sampled at admission after out-of-hospital cardiac arrest (OHCA) may predict AKI, mortality and unfavourable neurological outcome (UNO). Methods: Prospective observational study of resuscitated, comatose OHCA patients treated with targeted temperature management to 33°C for 24 hours at Oslo University Hospital Ulleval, Oslo, Norway. AKI was diagnosed during the first three days based on the Kidney Disease Improving Global Outcomes (KDIGO) guidelines. UNO defined as cerebral performance category 3-5 and mortality were assessed after six months. Urine samples were collected at hospital admission and analyzed for cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and the NephroCheck™ test calculating the product of tissue inhibitor of metalloproteinase 2 (TIMP-2) and insulin-like growth factor-binding protein 7 (IGFBP-7). Data are compared using the Mann-...
Delayed Coronary Endothelial Protection 24 Hours After Preconditioning
Authors: NathalieKaeffer, VincentRichard, ChristianThuillez
Journal: Circulation (1997)

Background Preconditioning (PC) induces delayed protection against myocardial ischemia/reperfusion (I/R) injury. Whether a similar late protective effect exists in coronary endothelial cells is not known. Thus, we assessed whether PC also induces late protection against endothelial injury after I/R and the potential role of reactive oxygen species as triggers for late PC in this setting. Methods and Results Rats were subjected to sham surgery or to PC with 1 cycle of 2 minutes I/5 minutes R and 2 cycles of 5 minutes I/5 minutes R in the absence or presence of the free radical scavenger N-2-mercaptopropionyl glycine (MPG). Twenty-four hours later, rats were subjected to 20 minutes I/60 minutes R in the absence or presence of MPG. At the end of R, coronary segments (diameter, 200 to 300 μm) were removed distal to the site of occlusion and mounted in wire myographs. I/R reduced the relaxations to acetylcholine (maximal relaxations: sham, 72±6%; I/R, 31±6%), and this impairment was prevented by MPG (64±7%). P...
Abstract 11871: Morphologic Features Predict Late Adverse Events in Uncomplicated Stanford Type-B Aortic Dissection
Authors: Anna M Sailer, Patty Nelemans, Sander M van Kuijk, Anne S Chin, Aya Kino, Peter Chiu, Michael P Fischbein, Michael D Dake, D C Miller, Geert W Schurink, Dominik Fleischmann
Journal: Circulation (2016)

Introduction: Medical treatment of uncomplicated Stanford type-B aortic dissection (AD) is associated with a high rate of late adverse events (AE). Endografting may prevent late complications, however, initial trials failed to demonstrate a survival benefit in the first two years. We hypothesize that morphologic features at the initial hospitalization are associated with AE and could aid in the risk stratification of patients with AD. Methods: Eighty-three patients with acute uncomplicated type-B AD were retrospectively identified at two aortic centers in the US and EU. Multiple clinical and morphologic features - including true vs. false lumen blood supply - were identified on high-quality CT scans and followed. Multivariable Cox proportional hazards regression was used to evaluate the associations between candidate predictors and AE - defined as rapid growth, aneurysm formation (≥6cm), organ ischemia, and fatal or non-fatal aortic rupture, and develop an internally validated risk score. Results: AE were observed in 33 patients over a median 850 (IQR 247-1824) days follow up. Five significant predictors were identified in the multivariable model: Connective tissue disease (HR 2.94, 95% CI: 1.29 - 6.72, p= 0.01), circumferential extent of false lumen in angular degrees (HR 1.03, 95% CI: 1.01 - 1.04, p= 0.003), maximum aortic diameter in mm (HR 1.10, 95% CI: 1.02 - 1.18, p= 0.015), false lumen outflow in mL/min (HR 0.999, 95% CI: 0.998 - 1.000, p= 0.055), and number of intercostal arteries (HR 0.89, 95% CI: 0.80 - 0.98), p= 0.024). A model combining these five factors was constructed to calculate patient specific risk scores allowing stratification into high, intermediate, and low risk terciles. The model showed good discriminatory ability with an optimism-corrected c-statistic of 70.1%. Conclusion: Morphologic features predict late AE in uncomplicated type-B AD. If externally validated this may identify high-risk patients who benefit from early endografting.
Abstract 14238: Label-free Imaging of Human Heart by Raman Microspectroscopy
Authors: Tsunehisa Yamamoto, Yoshinori Harada, Takeo Minamikawa, Sadahisa Yamaoka, Hitoshi Yaku, Tetsuro Takamatsu
Journal: Circulation (2013)

Introduction: The accurate elucidation of myocardial viability is a critical issue in determining the resection region of cardiac tissue in surgical ventricular restoration (SVR). In general, the myocardial viability is evaluated before the operation by using MRI and myocardial scintigraphy. These methods are difficult to apply for real time evaluation of the myocardial viability during the SVR operation. Raman spectroscopy is an ideal and valuable analytical tool in biological and medical fields, allowing identification of states of tissue via detecting molecular vibrations of intrinsic molecules in the tissue without labels or extensive preparations. In this study, we evaluated a new method of human cardiac tissue to identify cardiomyocytes from fibrotic tissue by Raman microspectroscopy for the first time. Method: Cardiac tissues were resected from five ischemic cardiomyopathy patients who underwent SVR. The resection stumps were immediately snap-frozen in liquid nitrogen and were sliced into 5-um-thic...
Number of Pregnancies and Atrial Fibrillation Risk
Authors: Jorge A. Wong, Kathryn M. Rexrode, Roopinder K. Sandhu, David Conen, Christine M. Albert
Journal: Circulation (2017)

Increased parity is independently associated with risk of cardiovascular disease (CVD) in large observational studies. 1 Although CVD is a strong risk factor for the development of atrial fibrillation (AF), 2 little is known about the relationship between parity and AF risk.To address these gaps in knowledge, and given the significant impact AF has in women, we sought to examine the relationship between parity and AF in a large cohort of women free of CVD and AF at baseline within the WHS (Women's Health Study).WHS began as a randomized trial examining the use of aspirin versus placebo for the primary prevention of CVD and cancer. 3The study enrolled 39 876 women aged ≥45 years without CVD or any major illness.Development of prespecified health outcomes, and updated demographic, lifestyle, and CVD risk factor information were captured in annual questionnaires.The randomized trial was completed in March 2004, and subjects were invited to participate in observational follow-up.Women reported their number of pregnancies lasting at least 6 months in duration at baseline and incident AF events beginning at 48 months and annually thereafter.Medical records were sought for all self-reported incident AF cases, and events were adjudicated by a committee of cardiologists.Only AF events confirmed by medical record review were included in the analysis.Patients who self-reported AF (n=876), CVD (n=14), or an unknown number of pregnancies at baseline (n=137) were excluded.We also excluded 4210 women who did not participate in observational follow-up because AF could not be reliably confirmed. 3The study population thus consisted of 34 639 women.All participants provided written informed consent, and the study was approved by Brigham and Women's Hospital institutional review board.The number of pregnancies ≥6 months was grouped into 5 categories (0 [referent], 1, 2-3, 4-5, ≥6) adapted from Ness et al. 1 Multivariable, time-updated, Cox proportional-hazards models were used to estimate the association between number of pregnancies and incident AF.Tests for linear trend were performed by assigning the median value to each pregnancy category and modeling this as a continuous variable in separate Cox models.All models were constructed without imputation for missing data.Person-time was calculated from return of the baseline questionnaire to the date of incident AF, death, loss to follow-up, or December 31, 2014, whichever occurred first.Median baseline age was 52.9 years (interquartile range, 48.9-58.8),and median number of pregnancies was 2 (interquartile range, 2-3).During a median follow-up of 20.5 years, 1532 incident AF cases occurred.The Table summarizes multivariable-adjusted hazard ratios and 95% confidence intervals for incident AF according to the number of pregnancies.After adjusting for age, there was a linear increase in the hazard ratio for incident AF across increasing parity categories (P-trend=0.004).
Improving on Tail-of-the-Curve Evaluation With the American Society of Clinical Oncology Value Framework
Authors: Nathan I. Cherny, Elisabeth G. E. de Vries
Journal: JAMA Oncology (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Oncology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Oncology journal
Abstract 19319: Fasting Glucose, Fasting Insulin, and Insulin Resistance in the Prediction of Myocardial Infarction and Mortality at Long-term Follow-up
Authors: Mette L Nielsen, Manan Pareek, Margrét Leósdóttir, Peter M Nilsson, Michael H Olsen
Journal: Circulation (2015)

Objective: To assess the additional prognostic value of fasting blood glucose (FBG), fasting plasma insulin (FPI), and homeostasis model assessment derived insulin resistance (HOMA-IR) for predicting incident myocardial infarction (MI) and all-cause mortality, independently of traditional cardiovascular (CV) risk factors. Methods: As part of a population-based cohort study aiming to screen for CV risk factors, 6024 men and 1013 women without known diabetes mellitus (DM) or overt CV disease (CVD defined as previous MI, stroke, or transient ischemic attack), recruited 1974-1992, had both FBG and FPI measured at baseline. Subsequently, HOMA-IR was derived using the computerized HOMA calculator and ranked into quartiles due to the non-normal distribution and presumably non-linear biological effect of insulin resistance. Prognostic values of FBG, FPI, HOMA-IR, and traditional risk factors were tested using Cox proportional hazards regression analysis and likelihood-ratio testing. Follow-up time from inclusion until event (first MI or death) or censoring (emigration or last follow-up date (≤ 20 years)) comprised the underlying time scale. Results: Median [IQR] age at inclusion was 47.7 [47.1-48.3] years, whereas median [IQR] HOMA-IR was 0.9 [0.4-1.4]. Over a median follow-up time of 20 years, 1448 events occurred (11.3 per 1000 person-years). The simple prediction model, i.e. the model with traditional CV risk factors only, included age, gender, body mass index, systolic blood pressure, total cholesterol, and smoking status. Addition of FBG, but not FPI or HOMA-IR resulted in significant model improvement (FBG: χ2 = 5.82, p = 0.02; FPI: χ2 = 0.29, p = 0.59; HOMA-IR: χ2 = 0.08, p = 0.78). Moreover, we detected significant interactions between both FBG and FPI (χ2 = 12.52, p &lt; 0.001), FBG and HOMA-IR (χ2 = 6.07, p = 0.01), and FPI and HOMA-IR (χ2 = 13.15, p &lt; 0.001). Conclusion: Addition of FBG, but not FPI or HOMA-IR provided additional significant prognostic value on top of traditional CV risk factors in the prediction of MI or death at long-term follow-up in subjects without CVD and/or DM at baseline. Furthermore, interaction analyses revealed that the prognostic values of both FBG and FPI were significantly greater among subjects in the highest HOMA-IR quartile.
Abstract 16081: Diastolic Function and Left Atrial Volume Improve Following Septal Myectomy for Obstructive Hypertrophic Cardiomyopathy
Authors: Albree Tower-Rader, Nicholas Furiasse, Jyothy Puthumana, Jane Kruse, Zhi Li, Adin-Cristian Andrei, Vera Rigolin, Robert O Bonow, Patrick M McCarthy, Lubna Choudhury
Journal: Circulation (2013)

Background: Septal myectomy is an established therapy for obstructive hypertrophic cardiomyopathy (HCM) patients with symptoms refractory to maximal medical therapy. Although reduction in left vent...
Automated human-level diagnosis of dysgraphia using a consumer tablet
Authors: Thibault Asselborn, Thomas Gargot, Łukasz Kidziński, Wafa Johal, David Cohen, Caroline Jolly, Pierre Dillenbourg
Journal: npj Digital Medicine (2018)

The academic and behavioral progress of children is associated with the timely development of reading and writing skills. Dysgraphia, characterized as a handwriting learning disability, is usually associated with dyslexia, developmental coordination disorder (dyspraxia), or attention deficit disorder, which are all neuro-developmental disorders. Dysgraphia can seriously impair children in their everyday life and require therapeutic care. Early detection of handwriting difficulties is, therefore, of great importance in pediatrics. Since the beginning of the 20th century, numerous handwriting scales have been developed to assess the quality of handwriting. However, these tests usually involve an expert investigating visually sentences written by a subject on paper, and, therefore, they are subjective, expensive, and scale poorly. Moreover, they ignore potentially important characteristics of motor control such as writing dynamics, pen pressure, or pen tilt. However, with the increasing availability of digital tablets, features to measure these ignored characteristics are now potentially available at scale and very low cost. In this work, we developed a diagnostic tool requiring only a commodity tablet. To this end, we modeled data of 298 children, including 56 with dysgraphia. Children performed the BHK test on a digital tablet covered with a sheet of paper. We extracted 53 handwriting features describing various aspects of handwriting, and used the Random Forest classifier to diagnose dysgraphia. Our method achieved 96.6% sensibility and 99.2% specificity. Given the intra-rater and inter-rater levels of agreement in the BHK test, our technique has comparable accuracy for experts and can be deployed directly as a diagnostics tool.
Correction to: Genome-wide discovery and characterization of maize long non-coding RNAs
Authors: Lin Li, Steven R. Eichten, Rena Shimizu, Katherine Petsch, Cheng-Ting Yeh, Wei Wu, Antony M. Chettoor, Scott A. Givan, Rex A. Cole, John E. Fowler, Matthew M. S. Evans, Michael J. Scanlon, Jianming Yu, Patrick S. Schnable, Marja C. P. Timmermans, Nathan M. Springer, Gary J. Muehlbauer
Journal: Genome biology (2018)

The original version [1] of this article unfortunately contained a mistake. The additive effects of the eQTLs of lncRNAs were flipped, meaning that the base allele in the contrast to derive the additive effects should have been B73, rather than Mo17, due to the original coding of biallele SNPs as "0s" and "1s". Going through the entire analysis procedure, it was determined that the mistake was made while tabulating the eQTL results from QTL Cartographer.
Abstract 19591: Long-term Renal Function Following Worsening Renal Function Precipitated by Aggressive Diuresis: Insights From the DOSE Trial Limited Dataset
Authors: Jennifer Simon, Isaac E Hall, Chukwuma O Onyebeke, Jennifer Schaub, Meredith A Brisco, Karen Modesto, Mahlet Assefa, Jeffrey M Testani
Journal: Circulation (2015)

Background: Worsening renal function (WRF) is a common complication of the treatment of acute decompensated heart failure and can be precipitated by aggressive diuresis. The DOSE trial randomized patients to a high vs. low intensity loop diuretic strategy. Notably, the rate of WRF during the 72-hour intervention period was increased with the high dose strategy but post-discharge non-renal outcomes were similar. However, the long term renal outcomes in patients with WRF precipitated by aggressive diuresis have not been described. Objective: To determine the long term renal outcomes of patients with WRF in the high and low dose arms of the DOSE trial. Methods: Participants of the DOSE trial with data on renal function at baseline and 60 days were studied. To maximize the number of events, WRF was defined as any worsening in estimated glomerular filtration rate (eGFR) from randomization to 72 hours. Results: In total, 224 patients (72.7%) in the DOSE trial population had data on renal function available at baseline and 60 days. Of this subset, 102 patients (45.5%) experienced WRF at 72 hours and these patients had an average 16.7 ± 12.0% worsening in eGFR. In patients with WRF that were randomized to the high dose strategy (n=58) eGFR improved from 44.9 ± 22.4 ml/min/1.73m 2 at 72 hours to 53.1 ± 30.0 ml/min/1.73m 2 (p&lt;0.001) at 60 days. Notably, eGFR was not significantly different at 60 days compared to baseline in these patients (p=0.18). However, in patients with WRF randomized to the low dose strategy (n=44), eGFR did not significantly improve from 72 hours to 60 days (46.4 ± 18.1 ml/min/1.73m 2 to 48.8 ± 22.4 ml/min/1.73m 2 , p=0.34) and eGFR at 60 days was significantly worse than baseline (p=0.02). Conclusion: In the DOSE trial, WRF precipitated by an aggressive loop diuretic strategy was transient and not associated with worse eGFR at 60 days whereas WRF in the absence of aggressive diuresis was associated with long term decline in renal function. The mechanism underlying WRF may be important in driving long term renal outcomes.