AB0242 Evaluation of variants in MIR-146A, MIR-196A-2 and MIR-499 and their association with susceptibility for rheumatoid arthritis and its extra-articular manifestations
Authors: JC De La Cruz-Castillejos, RE Barbosa-Cobos, LT Becerril-Mendoza, GE Lugo-Zamudio, J Ramírez-Bello, M Matias-Carmona, I Alemán-Άvila
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> The miRNAs, small non-coding RNA, regulate the genetic expression to posttranscriptional level, inhibiting the translation. The role of miRNAs in the evolution of RA is not clear. <h3>Objectives</h3> To evaluate the variants rs 2910164G/C in <i>miR-146a</i>, rs11614913C/T in <i>miR-196a-2</i> and rs3746444A/G in <i>miR-499</i> and their association with susceptibility and severiry to Rheumatoid arthritis (RA) and its extra-articular manifestations (EAM) <h3>Methods</h3> 133 cases with RA were included (ACR/EULAR criteria 2010) and 430 healthy controls. There were evaluated EAM (rheumatoid nodules [RN], Raynaud phenomenon [RP], cutaneous vasculitis (CV), episcleritis, scleritis, peripheral ulcerative keratitis [PUK], multiple mononeuritis [MM] and multiple polyneuritis [MP]) and levels of ESR, CRP, RF and CCP. It was performed genotyping of singe nucleotide polymorphisms (SNPs) rs2910164G/C in <i>miR-146a</i>, rs11614913C/T of <i>miR-196a-2</i> and rs3746444A/G of <i>miR-499</i>. The descriptive and inferential statistical analysis was performed with the software SPSS and Finetti. <h3>Results</h3> Patients with RA, women 126 (94.7%); age Me 48.9 (IQR 40–58); patients with EAM 23 (17.2%; women 22 [95.6%]; RN 14 [60.8%], RP 4 [17.3%], CV 1 [4.3%], episcleritis 1 [4.3%], PUK 1 [4.3%], MM 1 [4.3%], MP 1 [4.3%]); ESR Me 37 (IQR 22–45), CRP Me 0.11 (IQR 0.03–0.27); positive RF 125 patients (93.9%, high positive 106 [79.7%], low positive 19 [14.3%]; EAM with high positive RF, 100%), positive CCP 70 (52.6%, high positive 48.9%, low positive 3.8%; EAM high positive 94.1%, negative 5.9%). The alleles and genotypic frequencies did not show statistically significant difference between cases and the healthy controls (p&gt;0.05). It was identified statistical difference between the patients with and without EAM in CPR (p=0.032). The genotypic and allelic frequencies and association analysis of <i>miRNAs</i> in patients with and without EAM are shown in table 1 <h3>Conclusions</h3> None of the evaluated variants in <i>miRNAs</i> are associated with susceptibility for RA, however, the SNP re11614913C/T licated in <i>miR-196a-2</i> is associated with EAM. <h3>References</h3> Churov AV, Oleinik EK, Knip M. MicroRNAs in rheumatoid arthritis: altered expression and diagnostic potential. Autoimmun Rev 2015;14:1029–37. Singh RP, Massachi I, Manickavel S, Singh S, Rao NP, Hasan S et al. The role of miRNA in inflammation and autoimmunity. Autoimmun Rev 2013;12:1160–5. Nakasa T, Nagata Y, Yamasaki K, Ochi M. A mini-review: microRNA in arthritis. Physiol Genomics 2011;1:566–70. Chung IM, Ketharmathan S, Thiruvengadam M, Rajajumar G. Rheumatoid Arthritis: The Stride from Research to Clinical Practice. Int J Mol Sci 2016;17. piiE900. LI K, Tie H, Hu N, Chen H, Yin X, Peng C et al. Association of two polymorphisms rs2910164 in miRNA-146a and rs3746444 in miRNA-499 with rheumatoid arthritis: a meta-analysis. Hum Immunol 2014;75:602–8. Hashemi M, Eskandari-Nasab E, Zakeri Z, Atabaki M, Bahari G, Jahantigh. Association of pre-miRNA-146a rs2910164 and pre-miRNA-499 rs3746444 polymorphisms and susceptibility to rheumatoid arthritis. Mol Med Rep 2013;7:287–9. Lu L, Tu Y, Liu L, Qi J, He L. MicroRNA-499 rs3746444 polymorphism and autoimmune diseases risk: a meta-analysis. Mol Diagn Ther 2014;18:237–4. <h3>Disclosure of Interest</h3> None declared