tbuckley/cabot-search huggingface dataset:
Abstract 19424: Red Blood Cell Derived Microparticles Transfer Hemoglobin to Vascular Endothelial Cells, Activate the Heme Oxygenase System, and Mitigate Inflammatory Response
Authors: Adam Mitchell, Warren Gray, Islam Mohamed, Sheena Thomas, Kimberly Rooney, John D Roback, Charles D Searles
Journal: Circulation (2016)

Introduction:  Cell-derived microparticles (MPs) can shuttle cellular contents such as RNA or protein between cells, representing a novel mechanism of intercellular communication. Red blood cell-derived microparticles (RMPs) are among the most abundant MPs in human circulation and accumulate in stored human red blood cell transfusion units. Little is known regarding the effects of RMPs on vascular endothelial cells.   Hypothesis:  RMPs would be internalized by human aortic endothelial cells (HAECs), transfer RNA and protein cargo, and alter the response of cells to inflammatory stimuli.   Methods:  RMPs were isolated from stored human red blood cell transfusion units by centrifugation, labeled with a fluorescent membrane dye (PKH-26), and incubated with HAECs for 24 hours at a concentration of 6 x 10 6  MPs/mL, after which the cells were washed extensively. Internalization of RMPs by HAECs was assessed by confocal microscopy and flow cytometry. Monocyte adhesion to HAECs +/- LPS was assessed with a static monocyte adhesion assay, and levels of intracellular reactive oxygen species (ROS) +/- TBHP were measured with ROSstar. Changes in RNA and protein were assessed with RT-qPCR and Western blot, respectively.   Results:  Confocal microscopy and flow cytometry studies confirmed cellular internalization of RMPs by HAECs. RMPs significantly reduced monocyte adhesion to HAECs in the presence of LPS (0.53 fold, p   Conclusions:  RMPs transfer hemoglobin to HAECs in vitro, thereby activating the heme oxygenase pathway, which may account for the observed anti-inflammatory and antioxidative effects of RMPs on HAECs. These studies raise questions regarding the possibility of RMP mediated communication between red blood cells and the vascular endothelium, as well as the potential effects of RMPs in transfused blood.
TCTAP A-003 Prognosis and Predictors of Lesion Failure After Left Main Bifurcation Intervention: Pooled Analysis of Korean Multicenter Drug-eluting Stent Cohort
Authors: Tae-Min Rhee, Jung-Kyu Han, Han-Mo Yang, Kyung Woo Park, Hyun-Jae Kang, Bon-Kwon Koo, Hyo-Soo Kim
Journal: Journal of the American College of Cardiology (2018)
Divergent effects of RIP3 and MLKL inhibition on HF diet induced steatosis
Authors: W. Saeed, D.W. Jun, E. Kim, K.S. Jang, B.-K. Kang, E.C.J. Jang, S.B. Ahn, M.M. Kim, J.Y. Jeong, J.H. Sohn
Journal: Journal of Hepatology (2018)
Antibiotic-associated disruption of microbial composition and functionality is restored after fecal transplant in cirrhosis
Authors: J.S. Bajaj, A. Fagan, G. Kakiyama, T. Savidge, E. Gavis, M. Sikaroodi, D. Heuman, H. Nittono, H. Takei, Z. Kassam, M. Fuchs, P. Boonma, A. Haag, P. Gillevet
Journal: Journal of Hepatology (2018)
Biases in electronic health record data due to processes within the healthcare system: retrospective observational study
Authors: Denis Agniel, Isaac S Kohane, Griffin M Weber
Journal: BMJ (2018)

<h3>Abstract</h3> <h3>Objective</h3> To evaluate on a large scale, across 272 common types of laboratory tests, the impact of healthcare processes on the predictive value of electronic health record (EHR) data. <h3>Design</h3> Retrospective observational study. <h3>Setting</h3> Two large hospitals in Boston, Massachusetts, with inpatient, emergency, and ambulatory care. <h3>Participants</h3> All 669 452 patients treated at the two hospitals over one year between 2005 and 2006. <h3>Main outcome measures</h3> The relative predictive accuracy of each laboratory test for three year survival, using the time of the day, day of the week, and ordering frequency of the test, compared to the value of the test result. <h3>Results</h3> The presence of a laboratory test order, regardless of any other information about the test result, has a significant association (P&lt;0.001) with the odds of survival in 233 of 272 (86%) tests. Data about the timing of when laboratory tests were ordered were more accurate than the test results in predicting survival in 118 of 174 tests (68%). <h3>Conclusions</h3> Healthcare processes must be addressed and accounted for in analysis of observational health data. Without careful consideration to context, EHR data are unsuitable for many research questions. However, if explicitly modeled, the same processes that make EHR data complex can be leveraged to gain insight into patients' state of health.
Italian Nivolumab Expanded Access Program in Nonsquamous Non–Small Cell Lung Cancer Patients: Results in Never-Smokers and EGFR-Mutant Patients
Authors: Marina Chiara Garassino, Alain Jonathan Gelibter, Francesco Grossi, Rita Chiari, Hector Soto Parra, Stefano Cascinu, Francesco Cognetti, Daniele Turci, Livio Blasi, Carmelo Bengala, Enrico Mini, Editta Baldini, Silvia Quadrini, Giovanni Luca Ceresoli, Paola Antonelli, Enrico Vasile, Carmine Pinto, Gianpiero Fasola, Domenico Galetta, Marianna Macerelli, Diana Giannarelli, Giuseppe Lo Russo, Filippo de Marinis
Journal: Journal of Thoracic Oncology (2018)
Regulating Our Enthusiasm for Self-regulation Interventions
Authors: Laurence Steinberg
Journal: JAMA Pediatrics (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Repressing the repressor: Ezh2 mediates macrophage activation
Authors: Annette E. Neele, Menno P.J. de Winther
Journal: The Journal of Experimental Medicine (2018)

In this issue of JEM, Zhang et al. (https://doi.org/10.1084/jem.20171417) show that the suppressive epigenetic enzyme Ezh2 is an important regulator of macrophage activation. The absence of Ezh2 leads to reduced cytokine secretion and suppresses macrophage-dependent disease development. They identify the antiinflammatory factor Socs3 as an important target for Ezh2 and thus show that regulation of suppressive histone modifications controls macrophage activation in disease.
Relationship between Clinic and Ambulatory Blood-Pressure Measurements and Mortality
Authors: José R. Banegas, Luis M. Ruilope, Alejandro de la Sierra, Ernest Vinyoles, Manuel Gorostidi, Juan J. de la Cruz, Gema Ruiz-Hurtado, Julián Segura, Fernando Rodríguez-Artalejo, Bryan Williams
Journal: New England Journal of Medicine (2018)

Evidence for the influence of ambulatory blood pressure on prognosis derives mainly from population-based studies and a few relatively small clinical investigations. This study examined the associations of blood pressure measured in the clinic (clinic blood pressure) and 24-hour ambulatory blood pressure with all-cause and cardiovascular mortality in a large cohort of patients in primary care.We analyzed data from a registry-based, multicenter, national cohort that included 63,910 adults recruited from 2004 through 2014 in Spain. Clinic and 24-hour ambulatory blood-pressure data were examined in the following categories: sustained hypertension (elevated clinic and elevated 24-hour ambulatory blood pressure), "white-coat" hypertension (elevated clinic and normal 24-hour ambulatory blood pressure), masked hypertension (normal clinic and elevated 24-hour ambulatory blood pressure), and normotension (normal clinic and normal 24-hour ambulatory blood pressure). Analyses were conducted with Cox regression models, adjusted for clinic and 24-hour ambulatory blood pressures and for confounders.During a median follow-up of 4.7 years, 3808 patients died from any cause, and 1295 of these patients died from cardiovascular causes. In a model that included both 24-hour and clinic measurements, 24-hour systolic pressure was more strongly associated with all-cause mortality (hazard ratio, 1.58 per 1-SD increase in pressure; 95% confidence interval [CI], 1.56 to 1.60, after adjustment for clinic blood pressure) than the clinic systolic pressure (hazard ratio, 1.02; 95% CI, 1.00 to 1.04, after adjustment for 24-hour blood pressure). Corresponding hazard ratios per 1-SD increase in pressure were 1.55 (95% CI, 1.53 to 1.57, after adjustment for clinic and daytime blood pressures) for nighttime ambulatory systolic pressure and 1.54 (95% CI, 1.52 to 1.56, after adjustment for clinic and nighttime blood pressures) for daytime ambulatory systolic pressure. These relationships were consistent across subgroups of age, sex, and status with respect to obesity, diabetes, cardiovascular disease, and antihypertensive treatment. Masked hypertension was more strongly associated with all-cause mortality (hazard ratio, 2.83; 95% CI, 2.12 to 3.79) than sustained hypertension (hazard ratio, 1.80; 95% CI, 1.41 to 2.31) or white-coat hypertension (hazard ratio, 1.79; 95% CI, 1.38 to 2.32). Results for cardiovascular mortality were similar to those for all-cause mortality.Ambulatory blood-pressure measurements were a stronger predictor of all-cause and cardiovascular mortality than clinic blood-pressure measurements. White-coat hypertension was not benign, and masked hypertension was associated with a greater risk of death than sustained hypertension. (Funded by the Spanish Society of Hypertension and others.).
Report from the SWOG Radiation Oncology Committee: Research Objectives Workshop 2017
Authors: Paul Okunieff, Katherine Casey-Sawicki, Natalie A. Lockney, Bradford S. Hoppe, Heiko Enderling, Chelsea Pinnix, James Welsh, Sunil Krishnan, Greg Yothers, Martin Brown, Susan Knox, Robert Bristow, Paul Spellman, Timur Mitin, Nima Nabavizadeh, Jerry Jaboin, H. Charles Manning, Felix Feng, Susan Galbraith, Abhishek A. Solanki, Matthew M. Harkenrider, Richard Tuli, Roy H. Decker, Steven E. Finkelstein, Charles C. Hsu, Chul S. Ha, Reshma Jagsi, Dean Shumway, Megan Daly, Tony J.C. Wang, Thomas J. Fitzgerald, Fran Laurie, David T. Marshall, David Raben, Louis Constine, Charles R. Thomas, Lisa A. Kachnic
Journal: Clinical Cancer Research (2018)

Abstract The Radiation Therapy Committee of SWOG periodically evaluates its strategic plan in an effort to maintain a current and relevant scientific focus, and to provide a standard platform for future development of protocol concepts. Participants in the 2017 Strategic Planning Workshop included leaders in cancer basic sciences, molecular theragnostics, pharmaceutical and technology industries, clinical trial design, oncology practice, and statistical analysis. The committee discussed high-priority research areas, such as optimization of combined modality therapy, radiation oncology–specific drug design, identification of molecular profiles predictive of radiation-induced local or distant tumor responses, and methods for normal tissue-specific mitigation of radiation toxicity. The following concepts emerged as dominant questions ready for national testing: (i) what is the role of radiotherapy in the treatment of oligometastatic, oligorecurrent, and oligoprogressive disease? (ii) How can combined modality therapy be used to enhance systemic and local response? (iii) Can we validate and optimize liquid biopsy and other biomarkers (such as novel imaging) to supplement current response criteria to guide therapy and clinical trial design endpoints? (iv) How can we overcome deficiencies of randomized survival endpoint trials in an era of increasing molecular stratification factors? And (v) how can we mitigate treatment-related side effects and maximize quality of life in cancer survivors? The committee concluded that many aspects of these questions are ready for clinical evaluation and example protocol concepts are provided that could improve rates of cancer cure and quality of survival. Clin Cancer Res; 24(15); 3500–9. ©2018 AACR.
TCTAP C-197 Optical Coherence Tomography Defined Plaque Erosion After Removal of Coronary Guidewire
Authors: Sang-woong Choi, Seung-Ho Hur, Han-Jun Bae, Seungwoon Jun, In-Cheol Kim, Yun-Kyeong Cho, Hyoung-Seob Park, Hyuck-Jun Yoon, Hyungseop Kim, Chang-Wook Nam, Seong-Wook Han, Yoon-Nyun Kim, Kwon-Bae Kim
Journal: Journal of the American College of Cardiology (2018)
Normalization of NK cell phenotype is delayed after successful interferon-free therapy in cirrhotic patients with chronic HCV infection
Authors: E. Perpiñan, S.P. del Pulgar, M.C. Londoño, Z. Mariño, S. Lens, C. Bartres, A. Pla, P. González, M. García-López, E. Pose, X. Forns, M. Maini, G. Koutsoudakis
Journal: Journal of Hepatology (2018)
Exercise-induced increase in brain-derived neurotrophic factor in human Parkinson's disease: a systematic review and meta-analysis
Authors: Mark A. Hirsch, Erwin E. H. van Wegen, Mark A. Newman, Patricia C. Heyn
Journal: Translational Neurodegeneration (2018)

Animal models of exercise and Parkinson’s disease (PD) have found that the physiologic use of exercise may interact with the neurodegenerative disease process, likely mediated by brain derived neurotrophic factor (BDNF). No reviews so far have assessed the methodologic quality of available intervention studies or have bundled the effect sizes of individual studies on exercise-induced effects on BDNF blood levels in human PD. We searched MEDLINE, EMBASE, Cochrane Library, PsycINFO and PubMed from inception to June 2017. Data aggregated from two randomized controlled trials and four pre-experimental studies with a total of 100 ambulatory patients with idiopathic PD (Hoehn/Yahr ≤3) found improvements in BDNF blood concentration levels in all 6 studies (two RCTs and 4 pre-experimental studies). Pooled BDNF level change scores from the 2 RCTs resulted in a significant homogeneous summary effect size (Standardized Mean Difference 2.06, 95% CI 1.36 to 2.76), and a significant heterogeneous SES for the motor part of the UPDRS-III examination (MD -5.53, 95% CI -10.42 to -0.64). Clinical improvements were noted in all studies using a variety of outcome measures. The evidence-base consists primarily of small studies with low to moderate methodological quality. This review provides preliminary evidence for the effectiveness of physical exercise treatments for persons with PD on BDNF blood levels. Further research is needed.
Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche
Authors: Maria Maryanovich, Ali H. Zahalka, Halley Pierce, Sandra Pinho, Fumio Nakahara, Noboru Asada, Qiaozhi Wei, Xizhe Wang, Paul Ciero, Jianing Xu, Avigdor Leftin, Paul S. Frenette
Journal: Nature Medicine (2018)
Quadriceps tendon autograft for arthroscopic knee ligament reconstruction: use it now, use it often
Authors: Andrew J Sheean, Volker Musahl, Harris S Slone, John W Xerogeanes, Danko Milinkovic, Christian Fink, Christian Hoser
Journal: British Journal of Sports Medicine (2018)

Traditional bone-patellar tendon-bone and hamstring tendon ACL grafts are not without limitations. A growing body of anatomic, biomechanical and clinical data has demonstrated the utility of quadriceps tendon autograft in arthroscopic knee ligament reconstruction. The quadriceps tendon autograft provides a robust volume of tissue that can be reliably harvested, mitigating the likelihood of variably sized grafts and obviating the necessity of allograft augmentation. Modern, minimally invasive harvest techniques offer the advantages of low rates of donor site morbidity and residual extensor mechanism strength deficits. New data suggest that quadriceps tendon autograft may possess superior biomechanical characteristics when compared with bone-patella tendon-bone (BPTB) autograft. However, there have been very few direct, prospective comparisons between the clinical outcomes associated with quadriceps tendon autograft and other autograft options (eg, hamstring tendon and bone-patellar tendon-bone). Nevertheless, quadriceps tendon autograft should be one of the primary options in any knee surgeon's armamentarium.
malERA: An updated research agenda for characterising the reservoir and measuring transmission in malaria elimination and eradication
Journal: PLoS Medicine (2017)

This paper summarises key advances in defining the infectious reservoir for malaria and the measurement of transmission for research and programmatic use since the Malaria Eradication Research Agenda (malERA) publication in 2011. Rapid and effective progress towards elimination requires an improved understanding of the sources of transmission as well as those at risk of infection. Characterising the transmission reservoir in different settings will enable the most appropriate choice, delivery, and evaluation of interventions. Since 2011, progress has been made in a number of areas. The extent of submicroscopic and asymptomatic infections is better understood, as are the biological parameters governing transmission of sexual stage parasites. Limitations of existing transmission measures have been documented, and proof-of-concept has been established for new innovative serological and molecular methods to better characterise transmission. Finally, there now exists a concerted effort towards the use of ensemble datasets across the spectrum of metrics, from passive and active sources, to develop more accurate risk maps of transmission. These can be used to better target interventions and effectively monitor progress toward elimination. The success of interventions depends not only on the level of endemicity but also on how rapidly or recently an area has undergone changes in transmission. Improved understanding of the biology of mosquito–human and human–mosquito transmission is needed particularly in low-endemic settings, where heterogeneity of infection is pronounced and local vector ecology is variable. New and improved measures of transmission need to be operationally feasible for the malaria programmes. Outputs from these research priorities should allow the development of a set of approaches (applicable to both research and control programmes) that address the unique challenges of measuring and monitoring transmission in near-elimination settings and defining the absence of transmission.
Risk Factors and Biomarkers for the Occurrence of Uveitis in Juvenile Idiopathic Arthritis
Authors: Christoph Tappeiner, Jens Klotsche, Claudia Sengler, Martina Niewerth, Ina Liedmann, Karoline Walscheid, Miha Lavric, Dirk Foell, Kirsten Minden, Arnd Heiligenhaus
Journal: Arthritis & Rheumatology (2018)

To analyze the prognostic value of demographic, clinical, and therapeutic factors and laboratory biomarkers and to assess their role in predicting uveitis occurrence in patients with juvenile idiopathic arthritis (JIA).Patients with JIA were enrolled within the first year after JIA diagnosis. Demographic and clinical parameters were documented. Serum samples were collected at study enrollment, at 3-month follow-up visits within the first year, and then every 6 months. A multivariable Cox regression analysis was performed to evaluate the impact of demographic, clinical, laboratory, and therapeutic parameters on uveitis onset.We included 954 JIA patients (67.2% female, 54.2% antinuclear antibody [ANA] positive, mean ± SD age at onset 7.1 ± 4.6 years). Uveitis occurred in 133 patients (observation period 44.5 months). Young age at JIA onset and ANA positivity were significantly associated with the onset of uveitis (both P < 0.001). Treatment of arthritis with methotrexate alone (hazard ratio [HR] 0.18 [95% confidence interval (95% CI) 0.12-0.29], P < 0.001) or combined with etanercept (HR 0.10 [95% CI 0.04-0.23], P < 0.001) or adalimumab (HR 0.09 [95% CI 0.01-0.61], P = 0.014) reduced the risk of uveitis onset and the occurrence of uveitis-related complications. Predictors of uveitis onset included elevated erythrocyte sedimentation rate at baseline (HR 2.36 [95% CI 1.38-4.02], P = 0.002) and continuing moderate or high disease activity during follow-up as measured by the 10-joint clinical Juvenile Arthritis Disease Activity Score (HR 4.30 [95% CI 2.51-7.37], P < 0.001). Additionally, S100A12 levels ≥250 ng/ml at baseline were significantly associated with the risk of uveitis (HR 2.10 [95% CI 1.15-3.85], P = 0.016).Apart from demographic risk factors and treatment modalities, JIA disease activity scores and laboratory biomarkers could be used to better define the group of JIA patients at high risk of uveitis onset.