tbuckley/cabot-search huggingface dataset:
Graft‐infiltrating PD‐L1hi cross‐dressed dendritic cells regulate antidonor T cell responses in mouse liver transplant tolerance
Authors: Yoshihiro Ono, Angelica Perez‐Gutierrez, Toshimasa Nakao, Helong Dai, Geoffrey Camirand, Osamu Yoshida, Shinichiro Yokota, Donna Beer Stolz, Mark A. Ross, Adrian E. Morelli, David A. Geller, Angus W. Thomson
Journal: Hepatology (2017)

Although a key role of cross‐dressing has been established in immunity to viral infection and more recently in the instigation of transplant rejection, its role in tolerance is unclear. We investigated the role of intragraft dendritic cells (DCs) and cross‐dressing in mouse major histocompatibility complex (MHC)‐mismatched liver transplant tolerance that occurs without therapeutic immunosuppression. Although donor interstitial DCs diminished rapidly after transplantation, they were replaced in the liver by host DCs that peaked on postoperative day (POD) 7 and persisted indefinitely. Approximately 60% of these recipient DCs displayed donor MHC class I, indicating cross‐dressing. By contrast, only a very minor fraction (0%‐2%) of cross‐dressed DCs (CD‐DCs) was evident in the spleen. CD‐DCs sorted from liver grafts expressed much higher levels of T cell inhibitory programed death ligand 1 (PD‐L1) and high levels of interleukin‐10 compared with non–CD‐DCs (nCD‐DCs) isolated from the graft. Concomitantly, high incidences of programed death protein 1 (PD‐1)hi T cell immunoglobulin and mucin domain containing 3 (TIM‐3)+ exhausted graft‐infiltrating CD8+ T cells were observed. Unlike nCD‐DCs, the CD‐DCs failed to stimulate proliferation of allogeneic T cells but markedly suppressed antidonor host T cell proliferation. CD‐DCs were much less evident in allografts from DNAX‐activating protein of 12 kDa (DAP12)−/− donors that were rejected acutely. Conclusion: These findings suggest that graft‐infiltrating PD‐L1hi CD‐DCs may play a key role in the regulation of alloimmunity and in the induction of liver transplant tolerance. (Hepatology 2018;67:1499‐1515)
AB0929 Balance in fibromyalgia is not related with lower limb strength
Authors: P Serra-Añό, M Aguilar-Rodríguez, S Cortés-Amador, GV Espí-lόpez, N Sempere-Rubio
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Postural control, that depends on many sensory contributors and motor skills, is altered in people with fibromyalgia (FM) <sup>1–3</sup>. Knowing those contributors that could be impaired in this population is important to design an appropriate balance therapy. <h3>Objectives</h3> Due to the fact that people with FM use to present a strength loss <sup>7</sup>, our main goal was to explore the relation between postural control and lower limb strength in this population. Further, the sensory inputs contributors to the postural control were assessed. <h3>Methods</h3> A cross-sectional study was conducted in a sample of 40 FM patients. A force platform was used to assess postural control under five bipedal conditions: i) eyes-open (EO); ii) dual task (recalling a typical day in their life) (DT); iii) eyes-close (EC); iv) eyes-open over a foam (FEO); v. eyes-close over a foam (FEC). Three variables, "Ellipse", "RMS" and "Sample Entropy" (SampEn), were recorded (for both directions). Further, maximal quadriceps (qdc) and hamstring (hmsg) isometric strength were assessed using a dynamometer. Spearman correlations were conducted to establish the relationship between postural control and strength and a repeated-measures MANOVA, to analyze differences between conditions. <h3>Results</h3> When EO and DT (performed with eyes open) were compared, no significant differences were obtained. However when patients closed their eyes, an increase in ellipse and RMS_AP and a decrease in variability were obtained. The same was true when the tests were performed over a foam (altering proprioception), Table 1. Furthermore, no significant correlations (p&gt;0.05) were observed between strength and balance except for a weak significant correlation between hmsg strength and RMS_AP (r<sub>s</sub>=-0.33, p&lt;0.05) in the EC test. <h3>Conclusions</h3> Postural control worsens in FM people when some sensory inputs are altered or missed. Further, there is no correlation between lower limb strength and balance in this population. <h3>References</h3> Akkaya N, Akkaya S, Atalay NS, Acar M, Catalbas N, Sahin F. Assessment of the relationship between postural stability and sleep quality in patients with fibromyalgia. Clin Rheumatol. 2013;32(3):325–331. Jones KD, King LA, Mist SD, Bennett RM, Horak FB. Postural control deficits in people with fibromyalgia: a pilot study. Arthritis Res Ther. 2011;13(4):R127. Russek LN, Fulk GD. Pilot study assessing balance in women with fibromyalgia syndrome. Physiother Theory Pract. 2009;25(8):555–565. Gόes SM, Stefanello JM, Homann D, Lodovico A, Hubley-Kozey CL, Rodacki AL. Torque and muscle activation impairment along with insulin resistance are associated with falls in women with fibromyalgia. J Strength Cond Res Strength Cond Assoc. 2016; 30(11), 3155–3164. <h3>Acknowledgements</h3> Grant GV2016/140. Conselleria Educaciό Ciencia i esports. Valencia, Spain. <h3>Disclosure of Interest</h3> None declared
Hospital-Readmission Risk — Isolating Hospital Effects from Patient Effects
Authors: Harlan M. Krumholz, Kun Wang, Zhenqiu Lin, Kumar Dharmarajan, Leora I. Horwitz, Joseph S. Ross, Elizabeth E. Drye, Susannah M. Bernheim, Sharon-Lise T. Normand
Journal: New England Journal of Medicine (2017)

To isolate hospital effects on risk-standardized hospital-readmission rates, we examined readmission outcomes among patients who had multiple admissions for a similar diagnosis at more than one hospital within a given year.
Glomerular hyperfiltration is a predictor of adverse cardiovascular outcomes
Authors: Gianpaolo Reboldi, Paolo Verdecchia, Gioia Fiorucci, Lawrence J. Beilin, Kazuo Eguchi, Yutaka Imai, Kazuomi Kario, Takayoshi Ohkubo, Sante D. Pierdomenico, Joseph E. Schwartz, Lindon Wing, Francesca Saladini, Paolo Palatini
Journal: Kidney International (2017)
OP0136 Microrna-223-3p expression in affected skin of adult iga vasculitis correlates with the severity of skin involvement
Authors: A Hocevar, J Pizem, M Tomsic, D Glavac
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> IgA vasculitis (IgAV) represents a common systemic vasculitis in paediatric and adult population. Our current knowledge of disease pathogenesis is still very limited and there is no information on miRNAs profile in IgAV. <h3>Objectives</h3> The aim of our study was to determine the expression of three miRNAs (miR-148–3p, miR-155–5p and miR-223–3p) in the affected skin of adult IgAV patients. <h3>Methods</h3> The study included 65 skin samples from consecutive, untreated IgAV patients (61% male, median age 67.6 years, range 29–91), diagnosed between October 2014 and September 2016, and 10 control skin samples. Total RNA was isolated from tissue section of formalin-fixed, paraffin-embedded samples of biopsied IgAV vasculitic skin lesions and normal skin samples. Expression of miR-148–3p, miR-155–5p and miR-223–3p was measured using qRT-PCR. Skin miRNAs expression was then correlated to clinical characteristics of adult IgAV patients. To present relative miRNA expression the ΔΔCT method was used. <h3>Results</h3> We found significantly higher expression levels of miR-223–3p in the affected skin compared to controls (14-fold; p&lt;0.001). The expression of the 148b-3p and miR-155–5p was near normal levels (1.05-fold and 1.13-fold increase, respectively). The differences in the expression of miR-223–3p depending on clinical parameters of IgAV are presented in Table 1. Patients with necrotic skin lesions had significantly higher miR-223 tissue expression than those with non-necrotic purpura (p=0.020). Gastrointestinal tract (GIT) involvement inversely correlated with the level of skin miR-223 expression (p=0.024). No significant relationship between renal involvement and skin miR-223 was found. <h3>Conclusions</h3> miR-223 expression was increased in the affected skin of IgAV in comparison to normal skin. Levels of miR-223 expression correlated with severity of skin involvement and inversely with GIT involvement. <h3>Disclosure of Interest</h3> None declared
Abstract 633: Unique AF-Specific Symptom Score Assesses Long-Term Symptom Relief After Ablation
Authors: Anita Wokhlu, David O Hodge, Kristi Monahan, Janis Haroldson, Kelly J Wock, Samuel J Asirvatham, Paul A Friedman, Thomas M Munger, Douglas L Packer
Journal: Circulation (2008)

Introduction: Ablation is indicated in pts with highly symptomatic atrial fibrillation (AF). While trials of ablation report favorable rhythm and quality of life (QoL) outcomes, corroborative data ...
Metabolomic Profiling of Extracellular Vesicles and Alternative Normalization Methods Reveal Enriched Metabolites and Strategies to Study Prostate Cancer-Related Changes
Authors: Maija Puhka, Maarit Takatalo, Maria-Elisa Nordberg, Sami Valkonen, Jatin Nandania, Maria Aatonen, Marjo Yliperttula, Saara Laitinen, Vidya Velagapudi, Tuomas Mirtti, Olli Kallioniemi, Antti Rannikko, Pia R-M Siljander, Taija Maria Af Hällström
Journal: Theranostics (2017)

Body fluids are a rich source of extracellular vesicles (EVs), which carry cargo derived from the secreting cells. So far, biomarkers for pathological conditions have been mainly searched from their protein, (mi)RNA, DNA and lipid cargo. Here, we explored the small molecule metabolites from urinary and platelet EVs relative to their matched source samples. As a proof-of-concept study of intra-EV metabolites, we compared alternative normalization methods to profile urinary EVs from prostate cancer patients before and after prostatectomy and from healthy controls.We employed targeted ultra-performance liquid chromatography-tandem mass spectrometry to profile over 100 metabolites in the isolated EVs, original urine samples and platelets. We determined the enrichment of the metabolites in the EVs and analyzed their subcellular origin, pathways and relevant enzymes or transporters through data base searches. EV- and urine-derived factors and ratios between metabolites were tested for normalization of the metabolomics data.Approximately 1 x 1010 EVs were sufficient for detection of metabolite profiles from EVs. The profiles of the urinary and platelet EVs overlapped with each other and with those of the source materials, but they also contained unique metabolites. The EVs enriched a selection of cytosolic metabolites including members from the nucleotide and spermidine pathways, which linked to a number of EV-resident enzymes or transporters. Analysis of the urinary EVs from the patients indicated that the levels of glucuronate, D-ribose 5-phosphate and isobutyryl-L-carnitine were 2-26-fold lower in all pre-prostatectomy samples compared to the healthy control and post-prostatectomy samples (p < 0.05). These changes were only detected from EVs by normalization to EV-derived factors or with metabolite ratios, and not from the original urine samples.Our results suggest that metabolite analysis of EVs from different samples is feasible using a high-throughput platform and relatively small amount of sample material. With the knowledge about the specific enrichment of metabolites and normalization methods, EV metabolomics could be used to gain novel biomarker data not revealed by the analysis of the original EV source materials.
Nanoparticles of a polyaspartamide-based brush copolymer for modified release of sorafenib: In vitro and in vivo evaluation
Authors: Melchiorre Cervello, Giovanna Pitarresi, Antonella Bavuso Volpe, Barbara Porsio, Daniele Balasus, Maria Rita Emma, Antonina Azzolina, Roberto Puleio, Guido Ruggero Loria, Stefano Puleo, Gaetano Giammona
Journal: Journal of Controlled Release (2017)
FRI0676 A proposal for a simple ultrasound method for the diagnosis of early rheumatoid arthritis
Authors: T Serban, I Satulu, O Vutcanu, M Milicescu, CM Mihai, M Bojinca
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> At this point, the classification criteria for rheumatoid arthritis (RA) are well known and generally applied in clinical practice. (1) Ultrasound (US) assessment can help in distinguishing the patients with early RA (ERA) within the patients with early inflammatory arthritis (EIA). <h3>Objectives</h3> The aim of this study was to develop an US method for the diagnosis of ERA in a population of EIA patients from an Early Arthritis Research Center (EARC). <h3>Methods</h3> We have assessed all patients with EIA referred to our EARC between 2012–2016. Patients who were diagnosed with other diseases except EIA or ERA, or in whom symptom duration exceeded 12 months, were excluded from the analysis. Every patient underwent clinical, laboratory and ultrasound evaluation. For the proposed US diagnostic method we have evaluated bilaterally 3 joints: wrists, MCP II and III and 2 tendon regions: the extensor ulnaris carpis tendon and the flexor tendons of the fingers. The presence/absence of synovitis/tenosynovitis either in gray-scale or power-Doppler scale was scored in a binary mode as 1/0. In order to simplify the scanning protocol, we considered the flexor tendons of the fingers as a singular structure for each hand which meant that that the presence of US abnormalities in at least one flexor tendon was scored as 1. Thus, the maximum score obtainable in both hands was 10. We analyzed the performance of the proposed US method for the diagnosis of ERA using ROC curve analysis. <h3>Results</h3> Of 253 patients referred to our EARC, 73 satisfied the inclusion criteria; among them, 43 fulfilled the EULAR/ACR criteria for RA (ERA patients), while the other 30 were considered to have undifferentiated EIA. The demographic, clinical and US data of the 73 patients are displayed in the table below. 34/43 ERA patients (76.7%) had a duration of symptoms less or equal to 3 months which classifies them as very ERA (VERA). In ROC analysis, a cut-off of the US score of 4 had best results for sensitivity and specificity (73.3% and 82.1%, respectively), with an area under the curve of 0.812. The US score correlated with the levels of RF, ACPA, DAS28 and SDAI (p&lt;0.001), but not with those of acute phase reactants (p&gt;0.05). The time needed for performing the ultrasound examination was less than 10 minutes. <h3>Conclusions</h3> The proposed US method proves to be reliable in identifying patients with ERA. The binary mode of US evaluation allows even persons with little training in US examination to diagnose patients. As the costs and time needed for US evaluation are low, the method is valuable in clinical practice for a rapid assessment of patients with EIA. <h3>References</h3> Aletaha D et al. Arhritis Rheum. 2010;62:2569–258. <h3>Disclosure of Interest</h3> None declared
AB1122 All-cause of hospital mortality in patients with rheumatoid arthritis and lupus erythematosus in a university hospital during 1998 to 2014
Authors: D Vega-Morales, JA Esquivel Valerio, AC Arana-Guajardo
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> All-cause and cause-specific mortality is increased in patients with systemic lupus erythematosus (SLE) and Rheumatoid Arthritis (RA) when compared to the general population. Mortality can be attributed to the disease per se, side effects of drugs and effect of comorbidities. The survival of patients has improved over the past years when compared to historical controls <h3>Objectives</h3> The objective is to describe all-case of mortality in patients with SLE and RA in a university hospital. <h3>Methods</h3> This is an observational, descriptive, and cross-sectional study. We included all patients with SLE and RA hospitalized during 1998 to 2014. The cause of death was obtained from medical records and classified according to International Classification of Diseases (ICD)-10. We made a descriptive analysis of all-cause of mortality of both diseases. <h3>Results</h3> We analyzed 1,330 medical records, of which 215 died in hospital. The respiratory insufficiency was the most common mortality diagnosis in both diseases (RA 29%, SLE 24.1%), followed by sepsis (RA 25%, SLE 20.4%). The all-cause of mortality of SLE and RA are shown in Table 1. Of the 467 RA hospital admissions, the 5.1% died, and of the 863 SLE hospital admissions, the 22.1% died. <h3>Conclusions</h3> Advances in the diagnosis and treatment of SLE and RA, have decreased the morbidity and mortality of the two diseases. Infectious and cardiovascular pathologies were the most frequent causes of death. <h3>Disclosure of Interest</h3> None declared
Postoperative Antimicrobial Prophylaxis Following Cesarean Delivery in Obese Women
Authors: David P. Calfee, Amos Grünebaum
Journal: JAMA (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Refined karyotype-based prognostic stratification of chronic lymphocytic leukemia with a low- and very-low-risk genetic profile
Authors: I Del Giudice, G M Rigolin, S Raponi, L Cafforio, C Ilari, J Wang, M Bordyuh, A Piciocchi, M Marinelli, M Nanni, S Tavolaro, M Filetti, A Bardi, E Tammiso, E Volta, M Negrini, E Saccenti, F R Mauro, D Rossi, G Gaidano, A Guarini, R Rabadan, A Cuneo, R Foà
Journal: Leukemia (2017)
Oncologic Safety of Prophylactic Nipple-Sparing Mastectomy in a Population With <i>BRCA</i> Mutations
Authors: James W. Jakub, Anne Warren Peled, Richard J. Gray, Rachel A. Greenup, John V. Kiluk, Virgilio Sacchini, Sarah A. McLaughlin, Julia C. Tchou, Robert A. Vierkant, Amy C. Degnim, Shawna Willey
Journal: JAMA Surgery (2017)

Nipple-sparing mastectomy (NSM) offers superior cosmetic outcomes and has been gaining wide acceptance; however, its role among patients with BRCA mutations remains controversial.To report on the oncologic safety of NSM and provide evidence-based data to patients and health care professionals regarding preservation of the nipple-areolar complex during a risk-reducing mastectomy in a population with BRCA mutations.We retrospectively reviewed the outcomes of 9 institutions' experience with prophylactic NSM from 1968 to 2013 in a cohort of patients with BRCA mutations. Patients with breast cancer were included if they underwent contralateral risk-reducing mastectomy; however, only the prophylactic side was considered in the analysis. Patients found to have an occult primary breast cancer at the time of risk-reducing mastectomy, those having variant(s) of unknown significance, and those undergoing free nipple grafts were excluded.The primary outcome measure was development of a new breast cancer after risk-reducing NSM. Three reference data sources were used to model the expected number of events, and this was compared with our observed number of events.A total of 548 risk-reducing NSMs in 346 patients were performed at 9 institutions. The median age at NSM was 41 years (interquartile range, 34.5-47.5 years). Bilateral prophylactic NSMs were performed in 202 patients (58.4%), and 144 patients (41.6%) underwent a unilateral risk-reducing NSM secondary to cancer in the contralateral breast. Overall, 201 patients with BRCA1 mutations and 145 with BRCA2 mutations were included. With median and mean follow-up of 34 and 56 months, respectively, no ipsilateral breast cancers occurred after prophylactic NSM. Breast cancer did not develop in any patients undergoing bilateral risk-reducing NSMs. Using risk models for BRCA1/2 mutation carriers, approximately 22 new primary breast cancers were expected without prophylactic NSM. Prophylactic NSM resulted in a significant reduction in breast cancer events (test of observed vs expected events, P < .001).Nipple-sparing mastectomies are highly preventive against breast cancer in a BRCA population. Although the follow-up remains relatively short, NSM should be offered as a breast cancer risk-reducing strategy to appropriate patients with BRCA mutations.
AB0226 Functional disability in ra patients treated with biologics: hur-bio real life results
Authors: A Sari, B Armagan, A Erden, L Kilic, O Karadag, A Akdogan, S Apras Bilgen, S Kiraz, U Kalyoncu, I Ertenli
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Rheumatoid arthritis (RA) is a chronic, erozive disorder which may lead to permanent joints damage. Health assessment questionnaire (HAQ) is frequently used for evaluating functional disability in RA patients. <h3>Objectives</h3> The aim of this study is to determine the effect of biologic treatment on functional disability in RA patients. <h3>Methods</h3> HUR-BIO (Hacettepe University Rheumatology Biologic Registry) is a prospective, monocentric database of biological treatments including 1229 RA patients by August 2016. 523 patients in whom HAQ assessment before biologics was avaliable, were recruited in this retrospective analysis. HAQ score ≥1.0 was defined as severe functional disability.<sup>1</sup> HAQ assesment at last follow-up visit were evaluated. Demographic,clinical and serologic data of patients were also collected. Improvement of HAQ score 0,22 points or more was considered as clinically significant response to treatment.<sup>1</sup> <h3>Results</h3> Among 523 patients (80.5% female), mean age was 52.6±12.5 and mean disease duration was 9.4±7.3 years. Seropositivity for RF and/or CCP was present in 67.2% of patients. At baseline visit, HAQ score was ≥1.0 in 268 patients (51.2%). Baseline and last follow-up HAQ scores were 1.07±0.62 and 0.64±0.57. Minimal clinically significant improvement of HAQ score was observed in 238/377 patients (63.1%). Clinically significant response was more frequent in patients with baseline HAQ score of ≥1 (153/195 (78.4%) vs 85/182 (46.7%), p&lt;0.001). Table 1 represents features of patients according to baseline HAQ score. Mean follow-up time was 16.4±16.4 months. Data of at least one visit was available for 377 (72.0%) patients. <h3>Conclusions</h3> Functional disabilty was observed approximately half of patients. Clinically significant improvement was more frequent among patients with higher baseline HAQ scores particularly. Biologic treatment seems to be provide significant functional improvement. However significant functional disability persists in one fourth of patients. <h3>References</h3> Lillegraven S., Kvien T.K. Measuring disability and quality of life in established rheumatoid arthritis. Best Pract Res Clin Rheumatol. 2007;21:827–840. <h3>Disclosure of Interest</h3> None declared