tbuckley/cabot-search huggingface dataset:
Decrease in Lymphoid Specific Helicase and 5-hydroxymethylcytosine Is Associated with Metastasis and Genome Instability
Authors: Jiantao Jia, Ying Shi, Ling Chen, Weiwei Lai, Bin Yan, Yiqun Jiang, Desheng Xiao, Sichuan Xi, Ya Cao, Shuang Liu, Yan Cheng, Yongguang Tao
Journal: Theranostics (2017)

DNA methylation is an important epigenetic modification as a hallmark in cancer. Conversion of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) by ten-eleven translocation (TET) family enzymes plays an important biological role in embryonic stem cells, development, aging and disease. Lymphoid specific helicase (LSH), a chromatin remodeling factor, is regarded as a reader of 5-hmC. Recent reports show that the level of 5-hmC is altered in various types of cancers. However, the change in 5-hmC levels in cancer and associated metastasis is not well defined. We report that the level of 5-hmC was decreased in metastatic tissues of nasopharyngeal carcinoma, breast cancer, and colon cancer relative to that in non-metastasis tumor tissues. Furthermore, our data show that TET2, but not TET3, interacted with LSH, whereas LSH increased TET2 expression through silencing miR-26b-5p and miR-29c-5p. Finally, LSH promoted genome stability by silencing satellite expression by affecting 5-hmC levels in pericentromeric satellite repeats, and LSH was resistant to cisplatin-induced DNA damage. Our data indicate that 5-hmC might serve as a metastasis marker for cancer and that the decreased expression of LSH is likely one of the mechanisms of genome instability underlying 5-hmC loss in cancer.
Gene Regulation and Species-Specific Evolution of Free Flight Odor Tracking in Drosophila
Authors: Benjamin Houot, Laurie Cazalé-Debat, Stéphane Fraichard, Claude Everaerts, Nitesh Saxena, Sanjay P Sane, Jean-François Ferveur
Journal: Molecular Biology and Evolution (2017)

The flying ability of insects has coevolved with the development of organs necessary to take-off from the ground, generate, and modulate lift during flight in complex environments. Flight orientation to the appropriate food source and mating partner depends on the perception and integration of multiple chemical signals. We used a wind tunnel-based assay to investigate the natural and molecular evolution of free flight odor tracking in Drosophila. First, the comparison of female and male flies of several populations and species revealed substantial sex-, inter-, and intra-specific variations for distinct flight features. In these flies, we compared the molecular structure of desat1, a fast-evolving gene involved in multiple aspects of Drosophila pheromonal communication. We manipulated desat1 regulation and found that both neural and nonneural tissues affect distinct flight features. Together, our data suggest that desat1 is one of the genes involved in the evolution of free-flight odor tracking behaviors in Drosophila.
THU0624 Asessment of psychic experiences in patients with rheumatic diseases
Authors: JL Prados-Ojeda, JL Garrido-Castro, R Segura-Ruiz, C González-Navas, P Font-Ugalde, E Collantes-Estevez, A Escudero-Contreras
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> There are several studies that report psychiatric comorbidity in patients with rheumatic disease, mainly the presence of mood disorders. Some of them describe non-affective psychosis related with inflammatory processes. Population with chronic inflammatory disease could present subclinical psychotic experiences that can interfere in the patient9s functional status. The Community Assessment of Psychic Experiences (CAPE) scale is a validated and widely used tool for the evaluation of these experiences in the general population. <h3>Objectives</h3> To identify the presence of psychic experiences in different populations with a diagnosis of rheumatic disease, and to compare it with a sample of healthy subjects. <h3>Methods</h3> 124 subjects completed surveys including SF-12 and CAPE questionnaires, as well as other demographic and behavioral variables. Among them, 70 had Spondylarthritis (SpA) (age 44.3±13 years, 62% female), 23 rheumatoid arthritis (RA) (age 51.2±13 years, 82% female) and the rest were individuals without rheumatic diseases (47.6±12 years, 58% female). <h3>Results</h3> Results of the SF-12 test in their mental and physical domains, and the CAPE questionnaire in their dimensions (positive, negative, depressive and total symptoms) are shown in the table, expressed as mean value (SD) and in the graph expressed as density histograms with mean values. Significant statistical differences, according a t de Student test with control group is shown in both (* p&lt;0.05, ** p&lt;0.01). There were no significant differences in the mental component of the SF-12. These differences appear at the physical component, since patients have impaired their mobility and function due to their disease. About the CAPE questionnaire, patients had a little bit higher score due mainly to the appearance of depressive symptoms. The values of positive symptoms of psychosis remained within the normal range for diseases analyzed. <h3>Conclusions</h3> In our study, we found significant differences in the dimensions, especially depressive, of the CAPE scale among patients with rheumatic diseases (especially in SpA) and healthy subjects. This gives us an idea of the importance of considering the psychological problems of patients (anxiety, depression, ...) to improve the treatment of rheumatic disease. <h3>Acknowledgements</h3> We would like to thank these patients9 organizations for their collaboration in our study: Coordinadora Española de Asociaciones De Espondiloartritis (CEADE), Asociaciόn Cordobesa de Enfermos de Espondilitis (ACEADE), Asociaciόn Cordobesa de Enfermos de Artritis Reumatoide (ACOARE). <h3>Disclosure of Interest</h3> None declared
FRI0555 Left ventricular ejection fraction and bone mineral density after cardiac transplantation
Authors: L Bautista Aguilar, A Salmoral Chamizo, I Gomez Gracia, R Jimenez Gasco, P Font Ugalde, EC Lopez Medina, ML Ladehesa Pineda, L Perez Sanchez, A Escudero Contreras, E Collantes Estevez
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Left ventricular ejectionfraction (LVEF) has beendirectlyassociatedwith BMD in patientswithheartfailure. Nevertheless, no study has linked yet the left ventricular ejection fraction to bone mineral density and fragility fractures in cardiac transplantation. <h3>Objectives</h3> The main aim of this study was to evaluate the possible relationship between LVEF and BMD in heart transplantation and the association of LVEF with 25 OH vitamin D, paratohormone (PTH) and markers of bone remodeling in patients with heart transplantation (osteocalcin, telopeptide C terminal (CTX). <h3>Methods</h3> Seventy nine patients (66 male) were included in this cross-sectional study with a mean age of 55,75±14,81 years, body mass index (BMI) values of 26,95±5,35 kg/m<sup>2</sup> and an average post-transplantation period of 8,46±8,71 years. Transthoracic doppler echocardiography measuring LVEF (%) was calculated for each of patients, as well as bone mass study that included: bone mineral density scans of lumbar spine and hip, spine radiography, biomarkers of bone metabolism (calcium, phosphorus, osteocalcin,CTX,Parathyroid hormone and vitamin D). The association of LVEF with BMD and biomarkers of bone remodeling was determined by performing multiple linear regression analysis adjusted for variables directly related to BMD (age, sex, BMI, post-transplantation period and pharmacological treatment (daily corticoids dose, immunosuppressive treatment). <h3>Results</h3> A total of 79 patients were included in this present study. BMD in osteoporotic range was found in 31.2% of patients (17.7% in spine,16.52% in femoral neck and 13% in hip). Vitamin D deficiency (≤20ng/d) was detected in 68.4% of patients. Vertebral fracture was found in 30.4% and a 2.6% hip fracture. Bivariate analysis showed that the group of patients with FEVI ≤65% had a higher proportion of femoral neck osteoporosis (p=0.04), higher proportion of osteoporosis in total hip (p=0.03) and higher percentage of vertebral fractures (p=0.04) compared with group with LVEF&gt;65%. The multiple linear regression analysis indicated that LVEF was independently associated with osteoporosis in spine (B = -5.225, p=0.011), femoral neck osteoporosis (B = -5.411, p=0.015) and vertebral fractures (B = -5.433, p=0.002). In addition, LVEF was associated with osteocalcin levels (B = -0.105, p=0.002). <h3>Conclusions</h3> These results suggest that post-transplantation LVEF may have an influence on bone remodeling. However, further studies are neededin order to consider LVEF as a risk factor for osteoporosis and fractures due to fragility after heart transplantation. <h3>Disclosure of Interest</h3> None declared
AB1057 Multidisciplinary approach for diagnosing connective tissue disease-related lung disease: is it useful for rheumatologists?
Authors: S Peña Montelongo, A Monroy Calero, O Fernández Acosta, S Hernández García, L Martín Pérez, A Bonilla Arjona, H Pérez Sánchez, S Bustabad Reyes, B Rodríguez-Lozano
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Many patients with idiopathic interstitial pneumonia (IP) have certain clinical, serological and/or pulmonary morphologic features suggesting an underlying autoimmune disease (AD), but without meeting established criteria for connective tissue disease (CTD), a situation labelled as "IP with autoimmune features" (IPAF)<sup>1</sup>. To identify an underlying aetiology is important because it may impact on treatment and prognosis, and could be optimised by multidisciplinary approach. <h3>Objectives</h3> 1.To identify prevalence of IPAF in routine practice. 2. To determine the value of lung biopsy to diagnose underlying AD.3. To describe the course of IPAF. <h3>Methods</h3> Observational, longitudinal retrospective study in a tertiary hospital specific outpatient clinic for IP patients with cough and dyspnea as dominant symptoms requiring co-evaluation by rheumatologists in 2010–2016. Variables included: clinical, serologic and morphological findings by High-Resolution Computed Tomography of lungs assessed by 2011 current guidelines<sup>2</sup> and open lung biopsy. Statistical analysis:SPSS 17.0. <h3>Results</h3> Of 410 patients evaluated for IP, 93 had rheumatologist assessment, 70 F (75.3%), mean age at diagnosis 62.6 years (SD12.13), of whose 48 had no previous diagnosis. Mean follow-up 3.54 (SD2.77)years in undiagnosed patients. The most frequent radiological patterns were: inconsistent with usual interstitial pneumonia (UIP) (67.7%), UIP (22.6%), Possible UIP (4.3%), others (4.3%). Lung biopsy was performed in 15 patients (16%),11 without previous diagnosis. Histopathology patterns: 8 non-specific interstitial pneumonia (NSIP) in whom final rheumathologic clinical diagnosis was IPAF in 6 and UCTD in 2; 1 UIP (Sjögren); 1 vasculitis (p-ANCA) and 1 lymphoid interstitial pneumonia (LIP) corresponding to IPAF. We found lymphoid aggregates in one patient diagnosed as a IPAF. Overall new diagnostics were: IPAF 43.8%, UCTD 4.2%, CTD 52.1%.14/93 (15%) patients died, of whom 6 had IPAF (43%). <h3>Conclusions</h3> 1. Of patients with IP referred for rheumatologist assessment, 31% has no established CTD, with IPAF as clinical diagnosis in 24.7% of overall patients. 2. Surgical lung biopsy allowed to diagnose AD in 23% of unlabelled patients. 3. After a follow-up time of 3.5 years,43% of patients that died had been diagnosed as a IPAF. <h3>References</h3> Fischer A,et al. Er Respir J 2015. Raghu G, et al.Am J Respir Crit Care Med 2011. <h3>Disclosure of Interest</h3> None declared
AB1117 Latent tuberculosis infection and tuberculosis in patients with rheumatic diseases under treatment with anti-tumor necrosis factor drugs
Authors: ALB Morsch, G Garziera, F Otesbelgue, FL Staub, PE Palominos, CV Brenol, DR Silva
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> The introduction of biological agents, especially the tumor necrosis factor inhibitors (anti-TNF) for the treatment of rheumatic diseases increased the risk of developing tuberculosis (TB). Screening for latent TB infection (LTBI) is strongly recommended before starting therapy with anti-TNF agents. <h3>Objectives</h3> This study aimed to identify the prevalence of LTBI and TB among patients with rheumatic diseases on anti-TNF drugs. <h3>Methods</h3> In a cross-sectional study, the electronic medical records of all adult patients (≥18 years old) undergoing anti-TNF treatment at Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil, were reviewed. Every patient underwent Tuberculin Skin Test (TST) before starting anti-TNF treatment. <h3>Results</h3> In total, 176 patients were included. The mean age was 51.9±12.4 years, 34.7% were males, and 90.9% were white. The underlying diseases were rheumatoid arthritis (RA) in 50.6% (N=89), ankylosing spondylitis (AS) in 27.8% (N=49) and psoriatic arthritis (PsA) in 17.6% (N=31). Anti-TNF agents started after TST were: infliximab (22.7%, N=40), adalimumab (48.9%, N=86), etanercept (27.3%, N=48), and golimumab (1.1%, N=2). The prevalence of positive TST was 29.5%. Household contact with TB was significantly associated with a positive TST (p=0.020). RA patients had lower TST reactions than AS patients (p=0.022). There were six cases of TB (3.4%) diagnosed during anti-TNF therapy. <h3>Conclusions</h3> We demonstrated a high prevalence of positive TST (29.5%) among patients with rheumatic diseases in a region with high TB prevalence. Our data corroborates the ACR9s recommendation that patients who live in high TB incidence settings should be tested annually for LTBI. <h3>References</h3> Ishiguro T, Takayanagi N, Kagiyama N, Yanagisawa T, Sugita Y: Characteristics of tuberculosis in patients with rheumatoid arthritis: a retrospective single-center study. Intern Med 2014, 53: 1291–1298. Arkema EV, Jonsson J, Baecklund E, Bruchfeld J, Feltelius N, Askling J: Are patients with rheumatoid arthritis still at an increased risk of tuberculosis and what is the role of biological treatments? Ann Rheum Dis 2015, 74: 1212–1217. Singh JA, Furst DE, Bharat A, Curtis JR, Kavanaugh AF, Kremer JM et al.: 2012 update of the 2008 American College of Rheumatology recommendations for the use of disease-modifying antirheumatic drugs and biologic agents in the treatment of rheumatoid arthritis. Arthritis Care Res (Hoboken ) 2012, 64: 625–639. <h3>Disclosure of Interest</h3> None declared
AB0583 Differences between isolated aortitis and non-infectious aortitis secondary to other entities. study of 93 patients from a single center
Authors: N Vegas-Revenga, J Loricera, D Prieto-Peña, JL Hernández, I Martínez-Rodríguez, JI Banzo, M Calderόn-Goercke, V Calvo-Río, LC Domínguez-Casas, C González-Vela, P Fuentevilla, MΆ González-Gay, R Blanco
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Non-infectious aortitis is an inflammation of aortic wall which may be isolated or associated with a cluster of diseases. <h3>Objectives</h3> Our aim was to compare the clinical and laboratory findings of patients with isolated aortitis and patients with aortitis secondary to other underlying conditions. <h3>Methods</h3> Retrospective study of 93 patients with non-infectious aortitis diagnosed by PET/CT scan from a referral center from January 2010 to December 2016. We have considered two groups: <i>group a)</i> isolated aortitis; and <i>group b)</i> secondary aortitis. Distributions of <i>categorical variables were</i> compared by the Pearson Chi<sup>2</sup> or Fisher exact test. Quantitative variables were analyzing using the Student t test or Mann-Whitney U test as appropriate. <h3>Results</h3> Ninety-three patients were diagnosed with non-infectious aortitis. One patient was excluded due to missing data. Group a) was composed by 54 patients (34 women/ 20 men) with a mean age of 67±11 years; group b) comprised 38 patients (28 women/ 10 men) with a mean age of 68±11 years. In this group, the underlying conditions we found were: giant cell arteritis (n=24), Takayasu arteritis (n=3), spondiloarthropathy (n=3), Sjögren9s syndrome (n=3), ulcerative colitis (n=2), sarcoidosis (n=1), rheumatoid arthritis (n=1), polyarteritis nodosa (n=1). The comparative study between both groups is shown in the TABLE. Only inflammatory low back pain and polymyalgic syndrome yielded statistical signification. <h3>Conclusions</h3> In this study, we observed that both the presence of inflammatory low back pain and polymyalgic syndrome might have clinical relevance in the clinical suspicion of primary aortitis. However, larger studies are needed to corroborate these findings. <h3>Disclosure of Interest</h3> None declared
SAT0392 The diagnostic accuracy of existing grading criteria of sacroiliac joint ct in ankylosing spondylitis
Authors: D Gao, K Li, Y Wang, J Zhu, J Zhang, F Huang
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Imaging modalities are essential for the diagnosis of ankylosing spondylitis (AS) due to the absence of specific clinical manifestations. Sacroiliac Joint (SIJ) CT has been used to identify sacroiliitis for decades with a higher diagnostic accuracy than radiography in detecting structural changes, and not reducing the specificity like MRI. However, no well-accepted grading system for SIJ CT existed. <h3>Objectives</h3> We evaluated the diagnostic accuracy of existing grading criteria of sacroiliitis, aiming to provide references for future better reading of SIJ CT in AS. <h3>Methods</h3> A total of 2714 patients who had received CT scanning for any reasons with complete SIJ structures displaying between June 2012 and December 2015 were included. The CT scans were read by 2 rheumatologists together who had received professional training in radiology. Patients with sacroiliitis were selected and bilateral SIJs of each patient were evaluated separately by the 1984 modified New York (mNY) criteria, the criteria proposed by Lee (Lee criteria)<sup>[1]</sup> and the criteria from Innsbruck workshop report (Innsbruck criteria)<sup>[2]</sup>, respectively. The grading differences among these criteria were analyzed. <h3>Results</h3> Amount to 509 patients were detected having sacroiliitis with an average age of 34 years and 64% of male. Among 1018 SIJs of these patients, 45 SIJs graded 1∼3 by mNY criteria were graded 0 by Lee criteria, indicating the better specificity of Lee criteria. Lee criteria was much more convenient and reliable than mNY criteria for its more explicit definitions. The SIJs with definite sacroiliitis estimated by mNY and Lee criteria were 79.37% and 82.91%, respectively, and simply divided into grade 3 or grade 4. Conversely, 85.27% SIJs were identified as definite sacroiliitis and classified into 5 grades, from grade IIB to grade IVB, by Innsbruck criteria, and the percentages of each grade were 8.94%, 26.82%, 20.92%, 10.12% and 18.47%, respectively, which means a higher discrimination capability than the other two criteria. Other than graded by the extent of lesions in mNY criteria or Lee criteria, the grading assessment by Innsbruck criteria was based on the lesion types, which was more consistent with the natural progression of sacroiliitis. <h3>Conclusions</h3> Lee criteria has a better diagnostic specificity with a lower difficulty in the evaluation process, while Innsbruck criteria is a more detailed grading system, which has a higher consistency with the progression of sacroiliitis in AS. <h3>References</h3> Lee YH, et al. Rheumatol Int, 2013, 33(4): 1005–1011. Klauser A, et al. J Rheumatol 2004, 31(10): 2041–2047. <h3>Disclosure of Interest</h3> None declared
AB0454 Clinical and immunological activity in polish cohort of systemic lupus erythematosus patients treated with glucocorticoids
Authors: K Pawlak-Bus, P Leszczynski
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Nowadays the lupus treatment strategy is based on background therapy, immunosuppressive drugs and glucocorticoids (GC). Using minimal effective dose of GC only in flares is a recomandation for preventing complications which increase mortality. <h3>Objectives</h3> To evaluate SLE clinical and immunological activity in lupus patients during the standard clinical care and analyze GC treatment <h3>Methods</h3> We observed Polish cohort of patients with SLE 127 patients (118 female and 9 male) with average age 43±6 years, average disease duration 7,8±5,6 years. All of them were treated with oral and pulse GC and standard immunosuppressive therapies (CTX, MMF, AZT,MTX, CsA). As a background therapy 77% of these patients were on chloroquine or hydroksychloroquine. All patients were assessed according to Systemic Lupus Erythematosus Disease Activity Index assessed by SLEDAI (version 2000) and divided into 5 groups: no GC, low dose, medium dose, high dose and puls GC therapy group. Immunological activity was assessed by anti-dsDNA and C3 and C4 complements levels. <h3>Results</h3> In analyzed group 28 of patients wihout GC the average SLEDAI score was 7 and 50% of this pts not revealed any immunological activity. Low dose of GC was used in 50 pts with average SLEDAI score 13 and in 24 pts of this group anti-dsDNA and C3 or C4 levels upper limit were not observed. Medium dose of GC was used in 20 pts with average SLEDAI score 19 and it was contained with high immunological activity in 55% (n=11) of pts. In 27 of pts, high doses of GC including puls therapy were needed, the average SLEDAI score was very high 30 and most of pts from this group 70% were immunologicaly active. <h3>Conclusions</h3> In this Polish cohort lupus patients GC doses depended on lupus activity. Minimazing glucocorticoid exposure is an important part of appropriate management of lupus patients. Proper assessment of clinical and immunolgical lupus activity is critically for treatment decisions, especially for long-term GC use. <h3>Disclosure of Interest</h3> None declared