tbuckley/cabot-search huggingface dataset:
AB0227 Transcriptional profiling of synovial macrophages using minimally invasive ultrasound-guided synovial biopsies in rheumatoid arthritis
Authors: H. Perlman
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Despite the many therapies for patients with rheumatoid arthritis (RA), there is little information to guide selection of the most effective treatment for an individual patient. Forty-sixty percent of patients with RA respond (defined by ACR50 response criteria) to conventional disease modifying anti-rheumatic drugs (cDMARDs) or cDMARDs plus anti-tumour necrosis factor (TNF) therapy. Moreover, 20%–40% of subjects in clinical trials never demonstrate even a minimal response (ACR20 response criteria). Based on a population of over 300 million in the United States, a disease prevalence of 0.6%, and a course of 3–4 months per biologic DMARD therapy, as much as $2.5 billion is wasted annually on inadequate therapy. There is a clear need to develop precision-based therapy for patients with RA, whereby clinical information such as novel biomarkers will enhance our ability to predict the therapeutic response and thereby limit ineffective therapy. <h3>Objectives</h3> Currently, there are no reliable biomarkers for predicting therapeutic response in patients with rheumatoid arthritis (RA). The synovium may unlock critical information for determining efficacy as reduction in numbers of sublining synovial macrophages remains the most reproducible biomarker. Thus, a clinically actionable method for collection of synovial tissue, which can be analysed using high-throughput strategies, must become a reality. <h3>Methods</h3> Rheumatologists at six United States academic sites were trained in minimally invasive ultrasound-guided synovial tissue biopsy. Histology, fluorescence-activated cell sorting and RNA-seq were performed on biopsy synovial tissue from patients with RA and compared with osteoarthritis (OA) samples. An optimised protocol for digesting synovial tissue was developed to generate high quality RNA-seq libraries from isolated macrophage populations. Associations were determined between macrophage transcriptional profiles and clinical parameters of RA patients. <h3>Results</h3> Patients with RA reported minimal adverse effects in response to synovial biopsy. Comparable RNA quality was observed between synovial tissue and isolated macrophages from patients with RA and OA. Whole tissue samples from patients with RA demonstrated a high degree of transcriptional heterogeneity. In contrast, the transcriptional profile of isolated RA synovial macrophages highlighted a subpopulation of patients and identified six novel transcriptional modules that were associated with disease activity and therapy. <h3>Conclusions</h3> Performance of synovial tissue biopsies by rheumatologists in the United States is feasible and generates high-quality samples for research. By utilising cutting-edge technologies on synovial biopsies with corresponding clinical information, a precision-based medicine approach for patients with RA is attainable. <h3>Disclosure of Interest</h3> None declared
A Novel Inducible Mouse Model of <i>MLL‐ENL</i>‐driven Mixed‐lineage Acute Leukemia
Authors: Vaia Stavropoulou, Marwa Almosailleakh, Hélène Royo, Jean‐François Spetz, Sabine Juge, Laurent Brault, Patrick Kopp, Michelina Iacovino, Michael Kyba, Alexandar Tzankov, Michael B. Stadler, Gianni Cazzaniga, Antoine H.F.M. Peters, Juerg Schwaller
Journal: HemaSphere (2018)

Previous retroviral and knock-in approaches to model human t(11;19)+ acute mixed-lineage leukemia in mice resulted in myeloproliferation and acute myeloid leukemia not fully recapitulating the human disease. The authors established a doxycycline (DOX)-inducible transgenic mouse model "iMLL-ENL" in which induction in long-term hematopoietic stem cells, lymphoid primed multipotent progenitor cells, multipotent progenitors (MPP4) but not in more committed myeloid granulocyte-macrophage progenitors led to a fully reversible acute leukemia expressing myeloid and B-cell markers. iMLL-ENL leukemic cells generally expressed lower MLL-ENL mRNA than those obtained after retroviral transduction. Disease induction was associated with iMLL-ENL levels exceeding the endogenous Mll1 at mRNA and protein levels. In leukemic cells from t(11;19)+ leukemia patients, MLL-ENL mRNA also exceeded the endogenous MLL1 levels suggesting a critical threshold for transformation. Expression profiling of iMLL-ENL acute leukemia revealed gene signatures that segregated t(11;19)+ leukemia patients from those without an MLL translocation. Importantly, B220+iMLL-ENL leukemic cells showed a higher in vivo leukemia initiation potential than coexisting B220- cells. Collectively, characterization of a novel transgenic mouse model indicates that the cell-of-origin and the fusion gene expression levels are both critical determinants for MLL-ENL-driven acute leukemia.
SAT0515 Clinical characteristics of patients with cancer-associated myositis complicated by interstitial lung disease
Authors: Y. Kaneko, T. Nunokawa, Y. Taniguchi, Y. Yamaguchi, T. Gono, K. Masui, A. Kawakami, Y. Kawaguchi, S. Sato, M. Kuwana
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Cancer-associated myositis (CAM) is believed to be rarely complicated by interstitial lung disease (ILD), and, thus, detailed clinical characteristics of CAM in patients with polymyositis (PM)/dermatomyositis (DM)-associated ILD are not well known. <h3>Objectives</h3> To clarify the incidence, risk factors, and impact on survival of malignancy in patients with PM/DM-associated ILD, using a large cohort data. <h3>Methods</h3> We used 497 patients with PM/DM-associated ILD enrolled in a multicentre retrospective cohort of incident cases from 44 institutions across Japan (JAMI). CAM was defined as malignancy diagnosed within 3 years before or after PM/DM diagnosis. Demographic data and clinical characteristics were recorded at the time of diagnosis, and follow-up survival and malignancy data were collected prospectively. <h3>Results</h3> Thirty-two patients with CAM (6.4%) were identified. Patients in the CAM group were older (64.3 vs 55.1 years, p&lt;0.001), had shorter disease duration at onset (4.1 vs 7.0 months, p=0.01), and presented with arthritis less frequently (24.1 vs 48.5%, p=0.01), in comparison with those with non-CAM group. All patients with CAM were older than 40 years, and the proportion of CAM increased along with the age. Patients who were ≥59 years at diagnosis and lacked arthritis were at 12 times higher risk for concomitant malignancy than those without such features (figure 1). Frequencies of autoantibodies, including anti-MDA5, anti-ARS, and anti-TIF1gamma, were not different between the groups. In 19 patients (59%), malignancy was diagnosed within 3 months before or after PM/DM diagnosis. Eleven CAM patients were died, and cause of deaths included ILD in 6 and malignancy in 5. Survival analysis by Kaplan-Miere method demonstrated that CAM patients had a poorer survival than did non-CAM patients (p=0.016). <h3>Conclusions</h3> In patients with PM/DM-associated ILD, older age at diagnosis and lack of arthritis are predictors for concomitant malignancy, which leads to a reduced survival. <h3>Disclosure of Interest</h3> Y. Kaneko Speakers bureau: Astellas, T. Nunokawa: None declared, Y. Taniguchi: None declared, Y. Yamaguchi: None declared, T. Gono: None declared, K. Masui: None declared, A. Kawakami: None declared, Y. Kawaguchi: None declared, S. Sato Grant/research support from: a patent on anti-MDA5 antibody-measuring kit, M. Kuwana Grant/research support from: Astellas, Speakers bureau: Astellas, Japan Blood Products Organisation, Medical and Biological Laboratories
AB1458-HPR Work participation of people with musculoskeletal disorders in ireland: a qualitative multi-stakeholder analysis
Authors: K Robinson, K. O’Sullivan, A. Synnott
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Musculoskeletal disorders (MSD's) are a leading cause of work disability. Good work offers many potential benefits to employees with MSDs. Understanding the perspectives of all the players involved in helping people with MSD's to stay at, return to and remain in work will advance understanding of work related interventions and services. Given the varying health and social insurance systems across international contexts there is a need to develop contextually specific knowledge. <h3>Objectives</h3> To explore in-depth the perspectives of multiple stakeholders involved in helping people with MSD's stay at, return to or remain in work. <h3>Methods</h3> In-depth qualitative interviews were completed with people with MSD's, health professionals, and employers to explore their perspectives on what factors support people with MSD's to stay in, return to, or remain in work in Ireland. Data were analysed using thematic analysis. <h3>Results</h3> Across all stakeholder groups biological factors were most implicated as the reason for work absences and the resolution of symptoms was identified as the single greatest enabler of return to work. Although pockets of good practice were identified, in the main, health professionals and employers describe uncertainty about their role and responsibilities and describe a narrow scope of practice. Patients report a mostly adversarial experience of vocational supports. <h3>Conclusions</h3> Irish vocational rehabilitation stakeholders do not report awareness of the complex interplay of biological, psychological and social factors influencing work participation for people with MSD's. Vocational supports and services are hampered by role uncertainty and consequentially adversarial experiences for service users. <h3>Acknowledgements</h3> The authors acknowledge funding support from the Health Research Institute, Univeristy of Limerick for this study. <h3>Disclosure of Interest</h3> None declared
THU0672 Impact of pregnancy on physical function and health-related quality of life in women with axial spondyloarthritis
Authors: K. Ursin, S. Lydersen, J. Skomsvoll, M. Wallenius
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> The only previous study exploring the impact of pregnancy on health-related quality of life in women with axial spondyloarthritis (axSpA), included only ten women.<sup>1</sup> <h3>Objectives</h3> To prospectively study physical function and health-related quality of life in a large cohort of women with axSpA during and after pregnancy, using Bath Ankylosing Spondylitis Functional Index (BASFI) and three of the dimensions of RAND 36-Item Health Survey (RAND-36): Physical functioning, bodily pain and mental health. <h3>Methods</h3> RevNatus is a Norwegian nationwide register designed for the follow-up of pregnant women with rheumatic diseases. Our study comprised 179 pregnancies in 166 women with axSpA included in RevNatus between 2006 - 2016. The women had seven visits at a rheumatology unit; before pregnancy, in each trimester, and six weeks, six months and twelve months postpartum. BASFI-scores and scores of RAND-36 physical functioning, bodily pain and mental health from each visit were analysed in a linear mixed model. BASFI has an overall index score between 0 ("no functional impairment") and 10 ("maximal functional impairment").<sup>2</sup> The dimensions of RAND-36 have scores between 0–100 (where 100="best possible health").<sup>3</sup> <h3>Results</h3> BASFI-scores were highest in third trimester, significantly higher than six weeks postpartum (mean BASFI 3.6 vs. 2.6, p&lt;0.001). In line with this result, scores of RAND-36 physical functioning was significantly lower in third trimester compared to six weeks postpartum (mean physical function 54.5 vs. 71.0, p&lt;0.001). The women reported considerable pain throughout the study period, with worst reported pain in second trimester, when scores of RAND-36 bodily pain were significantly lower than six weeks after postpartum (mean bodily pain 44.3 vs. 49.6, p=0.01). Scores of RAND-36 mental health were high and stable throughout the study period, with best reported mental health six weeks after delivery, when mental health was significantly better than in all time points in pregnancy. <h3>Conclusions</h3> Even though women with axSpA reported considerable pain and gradually worsening of physical function in pregnancy, they experienced good and stable mental health from preconception to one year after delivery. <h3>References</h3> [1] Forger F, Ostensen M, Schumacher A, Villiger PM. Impact of pregnancy on health related quality of life evaluated prospectively in pregnant women with rheumatic diseases by the SF-36 health survey. Ann Rheum Dis2005;64(10):1494–9. [2] Sieper J, Rudwaleit M, Baraliakos X, Brandt J, Braun J, Burgos-Vargas R, et al. The Assessment of SpondyloArthritis international Society (ASAS) handbook: a guide to assess spondyloarthritis. Ann Rheum Dis2009;68(Suppl 2):ii1–44. [3] Hays RD, Sherbourne CD, Mazel RM. The RAND 36-Item Health Survey 1.0. Health Econ1993;2(3):217–27. <h3>Disclosure of Interest</h3> None declared
Youth Risk Behavior Surveillance — United States, 2017
Authors: Laura Kann, Tim McManus, William A. Harris, Shari L. Shanklin, Katherine H. Flint, Barbara Queen, Richard Lowry, David Chyen, Lisa Whittle, Jemekia Thornton, Connie Lim, Denise Bradford, Yoshimi Yamakawa, Michelle Leon, Nancy Brener, Kathleen A. Ethier
Journal: MMWR Surveillance Summaries (2018)

Health-risk behaviors contribute to the leading causes of morbidity and mortality among youth and adults in the United States. In addition, significant health disparities exist among demographic subgroups of youth defined by sex, race/ethnicity, and grade in school and between sexual minority and nonsexual minority youth. Population-based data on the most important health-related behaviors at the national, state, and local levels can be used to help monitor the effectiveness of public health interventions designed to protect and promote the health of youth at the national, state, and local levels.
SAT0489 Assesment of persistent organ damage according to imacs (INTERNATIONAL MYOSITIS ASSESMENT AND CLINICAL STUDIES) myositis damage index in 92 patients with idiopathic inflammatory myositis
Authors: E. Oguz, E. Sahin, M. Erdugan, B.A. Esen, A. Gul, L. Ocal, M. Inanc
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Number of work regarding long term organ damage caused by idiopathic inflamatory myopathies (IIM) and risk factors associated with organ damage have been undrstudied and never reported from Turkey; recently a new tool as developed fort that purpose. <h3>Objectives</h3> In this study we aimed to evaluate by long term organ damage and risk factors, associated with these prospectively in IIM patients. <h3>Methods</h3> IIM patients (n=110) who has been followed up for at least six month by our clinic and fulfilling Bohan and Peter criteria were recruited. Demographic data, clinical and serological features, treatment and final clinical tatus was recorded. IMACS Miyositis Damage Index (MDI) was determined twice in 92 patients (71% female) at the time of diagnosis from the records and at the at last clinical visit prospectively. <h3>Results</h3> Mean age of the 92 patients during the diagnosis was 46±14.7. Out of 92 patients 69% had dermatomyositis, 23% had polymyositis, 8% had necrotizing autoimmune myopathy and inclusion body myositis. Mean follow up was 82 months. Frequencies of disphagia, respiratuary muscle involvement and intertstitial lung disease were 29;5;34% respectively. Twenty-one percent of the patients had associated malignancy. The mean daily prednisolone dosage and total amount was 7.5 mg/day and 9000 mgs. Mortality was 13%. Initial mean MDI at the time of diagnosis was 1.6±3.0 (range, 0–14) and the last DMI score recorded was 6.1±4.7 (range, 0–21). After the last assessment the proportion of patients without damage was 8% and whose score was 4 were 37%. The last DMI score was significantly higher than the first DMI score (p&lt;0.001). The last DMI in females and patients with calcinosis were significantly high (p=0.002; p=0.007). The last DMI score and disease duration were weakly corelated (r=0.35 p=0.001). A moderately significant correlation was found between the last DMI score, the duration of glucocorticoid use and the total dose used (r=0.48 p&lt;0.001; r=0.45 p&lt;0.001). <h3>Conclusions</h3> Our long term follow up data showed that persistent organ damage assessed by DMI and mortality were high in patients with IIM and over half of patients developed severe damage. Organ damage was detected in some patients at presentation and DMI scores were significantly increased during the follow up. DMI scores were found high in females and in patients with calcinosis. There were significant correlation between disease duration, the duration of glucocorticoid use, the total dose used and DMI scores. Current treatments and strategies have been insufficient at improving the prognosis of patients with IIM and new treatment strategies and drugs are needed. <h3>Disclosure of Interest</h3> None declared
AB0592 Impairment in hand strength, dexterity and activities of daily living performance in patients with systemic lupus erythematosus: results of a cross-sectional study
Authors: K. Keramiotou, C. Anagnostou, A. Galanos, P. Sfikakis, M.G. Tektonidou
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Systemic lupus erythematosus (SLE) is a prototypic autoimmune disease affecting multiple systems. To date a small number of studies have assessed the hand function and performance of daily activities in SLE<sup>1</sup>. <h3>Objectives</h3> To examine the grip and pinch hand strength, the dexterity, and the performance of Activities of Daily Living (ADL) in SLE patients compared with healthy controls. <h3>Methods</h3> 197 SLE patients (48,03±12,76, 88,3% female) and 100 healthy controls (47,87±12,77, 86% female), matched by age and gender, were enrolled in the study. Both groups were assessed by hand grip and pinch grip strength, dexterity and ADL performance tests. Hand grip strength was measured by Jamar dynamometer, and pinch grip strength by pinch gauge, in both hands. Dexterity was measured by purdue pegboard test. Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire and Health Assessment Questionnaire (HAQ) were used in order to determine the difficulties in ADLs. <h3>Results</h3> Hand grip strength, both lateral and jaws pinch grip strength, and dexterity, were significantly impaired (p&lt;0,001) in both hands of SLE patients compared to healthy subjects (table 1). DASH questionnaire (19.78±20.93 vs 2.43±2.9, p&lt;0.001) and Health Assessment Questionnaire score (HAQ) (0.41±0.52 vs 0.03±0.52, p&lt;0.001) were also significantly different between SLE patients and healthy controls. <h3>Conclusions</h3> These findings demonstrate that SLE patients have lower grip, pinch strength and dexterity and more difficulties in ADL performance. These findings underline the need to develop specific hand therapy programs for SLE patients. <h3>References</h3> [1] Johnsson PM, Sandqvist G, Nilsson JA, et al. <i>Hand function and performance of daily activities in systemic lupus erythematosus: a clinical study</i>. Lupus2015;24:827–834 <h3>Disclosure of Interest</h3> None declared
Prenatal Ultrasonography and Autism Spectrum Disorder
Authors: Christoph Lees
Journal: JAMA Pediatrics (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Robotic-assisted endoscopic full-thickness resection of a gastrointestinal stromal tumor: the tip of the iceberg
Authors: Monica Saumoy, Brandon Guenthart, Luai Madanat, Cheguevara Afaneh, Michel Kahaleh
Journal: Endoscopy (2018)

A 56-year-old woman presented for evaluation of a gastric body submucosal nodule. She underwent endoscopic ultrasound, which revealed a hypoechoic lesion arising from the muscularis propria that measured 21.8 × 17.9 mm in diameter. Fine-needle aspiration demonstrated spindle cells positive for C-KIT and DOG-1, consistent with gastrointestinal stromal tumor (GIST).
Sexual harassment of US women in science is widespread and is worst in medicine, report says
Authors: Janice Hopkins Tanne
Journal: BMJ (2018)

Sexual harassment of women is rampant in US academic sciences and is worst in medicine, says a report by the National Academies of Sciences, Engineering, and Medicine.1 Systemic change is needed to create a diverse and welcoming scientific environment, it warns.

Harassment causes “significant damage to research integrity and a costly loss of talent” and should be considered “equally important as research misconduct in terms of its effect on the integrity of research,” the report said.

“Decades of research demonstrate how quality and innovation in business and science benefit from having a diverse workforce,” it said. “Thus, the cumulative effect of sexual harassment is a significant and costly loss of talent in academic science, engineering, and medicine, which has consequences for advancing the …
Disconnected pancreatic duct syndrome – Wait! Why not try one more time?
Authors: Shu-Ling Wang, Sheng-Bing Zhao, Tian Xia, Zhao-Shen Li, Yu Bai
Journal: Endoscopy (2018)

A 42-year-old man with a history of necrotizing pancreatitis complicated by pancreatic fluid collections (PFCs) who underwent percutaneous drainage for 4 months but still had a persistent external fistula with high amylase activity in the drainage fluid was referred. A previous endoscopic retrograde cholangiopancreatography (ERCP) in another endoscopy center had suggested complete main pancreatic duct (MPD) disruption ([Fig. 1]). Contrast injection through the drainage catheter showed no opacification of the proximal MPD ([Fig. 2 a]). During a second ERCP, carried out in our endoscopy center, contrast injection through the major duodenal papilla also demonstrated complete cutoff of the proximal MPD and no opacification of the distal MPD ([Fig. 2 b]). Therefore, the diagnosis of complete MPD disruption was made and normally surgical treatment would have been considered.