tbuckley/cabot-search huggingface dataset:
SAT0307 Recurrence rate of thrombosis for patients with anti-phospholipid antibodies initially and disappeared later after thrombosis
Authors: SM Kim, J Kim, Y Kim, SW Kang, S-C Shim, S-J Yoo
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> In case of anti-phospholipid syndrome, anticoagulants are recommended. However, there were no data about recurrence rate for thrombosis in patients with anti-phospholipid antibodies (APS) initially which disappeared later. <h3>Objectives</h3> We compared recurrence rate of thrombosis between negative conversion group and control group. <h3>Methods</h3> We reviewed the medical records of patients diagnosed with thrombosis such as cerebral infarct, myocardial infarct, deep vein thrombosis, or thrombosis of other vessels at a tertiary medical center from 2000 to October 2016. Of these, 14 patients whose APA status was converted from positive to negative after more than 12 weeks were enrolled as negative conversion group. Forty-six patients without APA were matched with the ratio of 1:3∼1:4 according to age, sex, thrombosis type (arterial or venous) and therapeutic agents as control group. <h3>Results</h3> There was no difference between negative conversion group and control group in smoking status, presence of diabetes or hypertension, duration from the thrombosis to last visits or to recurrence, the proportion of patients taking glucocorticoids. There was no difference in the overall recurrence of thrombosis between two groups [negative conversion group, 3/14 (21%) vs. control group, 6/46 (13%), p=0.423]. In the negative conversion group, the number of patients diagnosed with SLE was significantly higher than the control group [SLE, 3/14 (21%) vs. 1/46 (2%), p=0.036]. However, thrombosis had recurred in one out of three patients with SLE. <h3>Conclusions</h3> The cumulative incidence of thrombotic events was not significantly different between negative conversion group and control group. <h3>Disclosure of Interest</h3> None declared
AB0903 Laboratory findings in patients with chikungunya fever and chronic joint symptoms – a longitudinal assessment
Authors: A Ranzolin, RM Correia, HD de Lima, MRDA Freitas, AFR de Oliveira, ALBP Duarte, CDL Marques, PRS de Melo, LF da Rocha
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Chikungunya Fever (CF) is an arbovirosis with a high attack rate, affecting large proportion of the population in its outbreaks (85%&gt;90% of infected are symptomatic). In general, it is recommended to carry out laboratory tests when patients reach subacute phase or show signs of severity at the beginning of the disease. There are few studies showing which laboratory results are relevant and their clinical applicability. <h3>Objectives</h3> To recognize the most frequent findings of laboratory tests in a cohort of patients with CF and chronic joint symptoms and to correlate laboratory results with clinical data. <h3>Methods</h3> Patients with diagnosis of CF (clinical and epidemiological criteria) were followed in a cohort study. Clinical data and laboratory tests were collected in a regular schedule in the first months of the disease. <h3>Results</h3> A total of 54 patients were enrolled during 10 months, persistent changes in some patients were recorded (table). In the subacute phase, the ESR (erythrocyte sedimentation rate) correlated with number of swollen joints (r=0.45, p=0.03), VAS (visual analogue scale) of pain (r=0.72, p=0.0002), VAS patient9s general health (r=0.50 p=0.02), VAS by physician (r=0.45, p=0.03) and with HAQ (r=0.51, p=0.01). In subacute phase the VAS of morning stiffness correlated with CRP (r=0.46, p=0.02). In chronic phase, CRP correlated with VAS of pain (r=0.47, p=0.02) and there was a reversal in the correlations between ESR and VAS of general health of the patient (r=-0.54, p=0.03), VAS of physician (r=-0.52, p=0.02), swollen joints (r=-0.46 p=0.03) and HAQ (r=-0.56, p=0.01). ESR and SF-12 (mental component) were correlated (r=0.61, p=0.01). <h3>Conclusions</h3> Levels of ESR correlated with measures of pain and worsening of functional capacity in subacute phase. In chronic phase, there was reversal of this correlation, indicating that ESR does not reflect clinical worsening of patients at this stage. Further clinical studies are needed to better analize other alterations. <h3>References</h3> Weaver SC, Lecuit M. Chikungunya virus and the global spread of a mosquito-borne disease. N Engl J Med 2015 Mar 26;372(13):1231–9. Brito CA, Sohsten AK, Leitão CC et al. Pharmacologic management of pain in patients with Chikungunya: a guideline. Rev Soc Bras Med Trop. 2016; 49(6):668–679. <h3>Disclosure of Interest</h3> None declared
新規ホスファチジルプロドラッグのホスホリパーゼDベースのドラッグデリバリー戦略の設計と評価【Powered by NICT】
Authors: Tao Xinyi, Jia Ning, Cheng Nenghui, Ren Yuhong, Cao Xuni, Liu Min, Wei Dongzhi, Wang Feng-Qing
Journal: Biomaterials (2017)
Changes in Transportation-Related Air Pollution Exposures by Race-Ethnicity and Socioeconomic Status: Outdoor Nitrogen Dioxide in the United States in 2000 and 2010
Authors: Lara P. Clark, Dylan B. Millet, Julian D. Marshall
Journal: Environmental Health Perspectives (2017)

Disparities in exposure to air pollution by race-ethnicity and by socioeconomic status have been documented in the United States, but the impacts of declining transportation-related air pollutant emissions on disparities in exposure have not been studied in detail.
AB0102 Impairment of granzyme b-producing regulatory b cells exacerbated rheumatoid arthritis
Authors: L Xu, F Hu, X Liu, L Zhu, L Ren, H Liu, H Zhu, Y Su
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Rheumatoid arthritis (RA) is a common and complex autoimmune disease characterized by chronic inflammation and cartilage/bone damage involving numerous cells, such as T cells, B cells, chondrocytes, fibroblasts <sup>[1]</sup>. B cells had long been well-demonstrated to participant in the development of RA <sup>[2]</sup>. Except producing specific antibody and inducing T cell activation, impaired immunosuppressive function of B cells further emphasized their roles in RA recently <sup>[3]</sup>. <h3>Objectives</h3> To investigate whether B cells could produce granzyme B and the potential role in the pathogenesis of Rheumatoid arthritis (RA). <h3>Methods</h3> To reveal the expression of granzyme B in B cells, flow cytometry, PCR and Elispot were performed. The role of IL-21 and anti-BCR stimulation on granzyme B expression was assessed by in vitro stimulation assay. CD4<sup>+</sup> T cell-B cell co-culture in the presence of granzyme B neutralizing antibody was performed to demonstrate the function of these cells. Then the levels of granzyme B in B cells between RA patients, OA patients as well as HCs were compared. Next,the correlation analysis between granzyme B-producing B cells and clinical features in RA patients was performed. Finally, the frequencies of granzyme B-producing B cells in RA patients before and after therapy were also evaluated using flow cytometry. <h3>Results</h3> B cells could spontaneously produce granzyme B, which could be perpetuated by IL-21 and anti-BCR stimulation. The frequencies of Th1 and Th17 cells were significantly elevated under the condition of granzyme B blockade when granzyme B was neutralized in CD4<sup>+</sup> T cell-B cell co-culture. In RA patients, but not OA patients and HCs, the frequencies of granzyme-B producing Bregs decreased significantly, which was functionally impaired and negatively correlated with disease activity score 28. Moreover, after effective clinical therapy, the frequencies could recover to nomal levels. <h3>Conclusions</h3> B cells could exert the regulatory functions via granzyme B production. Under RA circumstance, these granzyme B-producing Bregs were impaired and contributed to the disease progression. <h3>References</h3> Smolen JS, Aletaha D, McInnes IB, Rheumatoid arthritis, Lancet. 2016 Oct 22;388(10055):2023–2038. doi: 10.1016/S0140–6736(16)30173–8. Nakken B, Munthe LA, Konttinen YT, et al,B-cells and their targeting in rheumatoid arthritis–current concepts and future perspectives. Autoimmun Rev. 2011 Nov;11(1):28–34. doi: 10.1016/j.autrev.2011.06.010. Daien CI, Gailhac S, Mura T, Regulatory B10 cells are decreased in patients with rheumatoid arthritis and are inversely correlated with disease activity. Arthritis Rheumatol. 2014 Aug;66(8):2037–46. doi: 10.1002/art.38666. <h3>Acknowledgements</h3> This study was supported by grants from the Natural Science Foundation of China (81671609, 81671604,31470039). <h3>Disclosure of Interest</h3> None declared
Toll-like receptor 7/8 agonists stimulate plasmacytoid dendritic cells to initiate TH17-deviated acute contact dermatitis in human subjects
Authors: Natalie Garzorz-Stark, Felix Lauffer, Linda Krause, Jenny Thomas, Anne Atenhan, Regina Franz, Sophie Roenneberg, Alexander Boehner, Manja Jargosch, Richa Batra, Nikola S. Mueller, Stefan Haak, Christina Groß, Olaf Groß, Claudia Traidl-Hoffmann, Fabian J. Theis, Carsten B. Schmidt-Weber, Tilo Biedermann, Stefanie Eyerich, Kilian Eyerich
Journal: Journal of Allergy and Clinical Immunology (2017)

BackgroundA standardized human model to study early pathogenic events in patients with psoriasis is missing. Activation of Toll-like receptor 7/8 by means of topical application of imiquimod is the most commonly used mouse model of psoriasis.ObjectiveWe sought to investigate the potential of a human imiquimod patch test model to resemble human psoriasis.MethodsImiquimod (Aldara 5% cream; 3M Pharmaceuticals, St Paul, Minn) was applied twice a week to the backs of volunteers (n = 18), and development of skin lesions was monitored over a period of 4 weeks. Consecutive biopsy specimens were taken for whole-genome expression analysis, histology, and T-cell isolation. Plasmacytoid dendritic cells (pDCs) were isolated from whole blood, stimulated with Toll-like receptor 7 agonist, and analyzed by means of extracellular flux analysis and real-time PCR.ResultsWe demonstrate that imiquimod induces a monomorphic and self-limited inflammatory response in healthy subjects, as well as patients with psoriasis or eczema. The clinical and histologic phenotype, as well as the transcriptome, of imiquimod-induced inflammation in human skin resembles acute contact dermatitis rather than psoriasis. Nevertheless, the imiquimod model mimics the hallmarks of psoriasis. In contrast to classical contact dermatitis, in which myeloid dendritic cells sense haptens, pDCs are primary sensors of imiquimod. They respond with production of proinflammatory and TH17-skewing cytokines, resulting in a TH17 immune response with IL-23 as a key driver. In a proof-of-concept setting systemic treatment with ustekinumab diminished imiquimod-induced inflammation.ConclusionIn human subjects imiquimod induces contact dermatitis with the distinctive feature that pDCs are the primary sensors, leading to an IL-23/TH17 deviation. Despite these shortcomings, the human imiquimod model might be useful to investigate early pathogenic events and prove molecular concepts in patients with psoriasis.Graphical abstract
CHAPTER X: Clinical and Electrocardiographic Observations
Journal: Circulation (1973)

Clinical findings were not sensitive indicators of the presence or resolution of pulmonary emboli. However, patients with massive pulmonary embolism who received urokinase tended to have more rapid resolution of dyspnea, ↑S 2 P, and gallop sounds (at 24 hours) and more complete resolution at 14 days than comparable heparin patients. The preinfusion electrocardiogram was abnormal in the great majority of patients. Rhythm disturbances were infrequent, but QRS and ST-T changes were present in the majority. The most frequent single finding was T-wave inversion. Much less frequent were those changes of classic acute cor pulmonale. The limitations of electrocardiographic changes, therefore, are related to the inverse relation of their frequency and specificity. Moreover, the electrocardiogram did not sensitively reflect the resolution of pulmonary emboli.
FRI0386 The validation of the scleroderma health assessment questionnaire in turkish speaking patients with systemic sclerosis
Authors: D Temiz Karadag, F Karakas, S Tekeoglu, O Işik Ozdemir, A Yazici, A Cefle
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> The Health Assessment Questionnaire (HAQ) is one of the main instruments for assessing disability in rheumatic diseases.The Scleroderma HAQ (S-HAQ) combines the HAQ with five systemic sclerosis (SSc) related visual analogue scales for Raynaud9s phenomenon (RP), digital ulcers, digestive symptoms, pulmonary symptoms, and overall disease severity. <h3>Objectives</h3> To perform cross-cultural adaptation and validation of the S-HAQ in patients with SSc. <h3>Methods</h3> Sixty patients who fulfilled the 2013 ACR/EULAR Classification Criteria for Systemic Sclerosis ACR criteria for SSc were were recruited.Fifty three percent of the patients fulfilled the criteria for limited systemic sclerosis (lcSSc).We evaluated test–retest reliability using the intraclass correlation coefficient (ICC); known-groups construct validity by stratifying patients according to severe organ involvement; and convergent validity using Spearman9s correlation with mental and physical components of Short Form 36 version 2 (SF36v2). <h3>Results</h3> Eighty percent of the patients were female and the median age was 56 years old.Finger tip ulcers were observed in 16 patients (26.7%) and severe organ involvements were detected as intersititial lung disease in 32 (53%) and pulmonary hypertension in 5 (8%) of the patients.HAQ Disability Index (HAQ-DI), digestive VAS, pulmonary VAS, digital ulcer VAS, Raynaud9s phenomenon VAS and overall disease severity VAS demonstrated high reliability (ICCs=0.819–0.908).The HAQ-DI showed higher correlation coefficients with physical function and role physical components of physical-related scores (r=-0.537 and – 0.453, respectively); social function and role emotional components of mental-related dimensions (r= -0.470 and -0.328, respectively) in the SF-36.Two among five SSc related VAS (VAS digital ulcer and VAS overall disease severity) were highly correlated with HAQ-DI (r=0.527 and 0.481 respectively).The instruments could not discriminate between severe organ involvements and both subsets of SSc. <h3>Conclusions</h3> The Turkish version of the S-HAQ meet the requirements of reproducibility and validity.More studies are needed to examine the capacity of these instruments to discriminate between severe involvements and disease subsets.This study forms the basis for future studies to evaluate the properties of S-HAQ in Turkish speaking SSc patients more extensively. <h3>References</h3> R Hesselstrand et al, Psychometric properties of the Swedish version of the Scleroderma Health Assessment Questionnaire and the Cochin Hand Function Scale in patients with systemic sclerosis, Scandjrheumatol Vol. 42, Iss. 4,2013. <h3>Disclosure of Interest</h3> None declared
AB0490 Analysis of common gram-negative bacteria and their drug resistance in hospitalized patients with systemic lupus erythematosus
Authors: D Chen, Z Zhan
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> Infection is an important cause of morbidity and mortality in patients with systemic lupus erythematosus (SLE). The spectrum of infectious agents in SLE patients varies significantly among different ethnic groups. The national surveillance study from the China showed that Gram-negative bacteria (GNB) was the most the common bacterial infection in China, while Gram-positive bacteria (GPB) was predominant in European countries. <h3>Objectives</h3> To identify the spectrum and drug resistant pattern of infection caused by GNB in patients hospitalized with SLE. <h3>Methods</h3> The clinical and microbiological data from hospitalized SLE patients with bacterial infection between June 2005 and June 2015 was collected and then analyzed retrospectively. <h3>Results</h3> Two hundred and sixty-eight episodes of bacteria had been identified from 3815 hospitalized patients. In terms of isolated microorganisms, gram-negative bacteria (GNB) were predominant over gram-positive bacteria (GPB) (178 isolates vs. 90 isolates). In the GNB, <i>Escherichia coli</i> (66/178, 37.1%) was the most common isolate, followed by <i>Acinetobacter baumannii</i> (36/178, 20.2%), <i>Klebsiella pneumoniae</i> (24/178, 13.5%), <i>Pseudomonas aeruginosa</i> (20/178, 11.2%), <i>Haemophilus influenzae</i> (10/178,5.6%), <i>Salmonella sp.</i> (7/178, 3.9%), <i>Enterobacter aerogenes</i> (5/178, 2.8%), <i>Stenotrophomonas maltophilia</i> (5/178, 2.8%), <i>Citrobacter freundii</i> (3/178,1.7%), <i>Proteus mirabilis</i> (2/178,1.1%). Resistant isolates (53/178, 30.0%) were more common documented in GNB, mostly extended-spectrum beta-lactamase (ESBL) producing <i>Escherichia coli</i> (30/66, 45.5%) and <i>Klebsiella pneumonia</i> (6/24, 25%), and multi-drug resistant <i>acinetobacter baumannii</i> (36.1%). Susceptibility tests showed that the ESBL-producing strains were highly sensitive to carbapenems, β-lactamase inhibitor compound families, and certain cefalosporin (Cefepime and Ceftazidime) <i>in vitro.</i> (the resistance rate &lt;20%), whereas it was highly resistant to ampicillin and Gentamicin. Besides carbapenems and Cefoperazone/sulbactam, <i>Acinetobacter baumannii</i> was resistant to most antibiotics. <h3>Conclusions</h3> GNB was predominant in Chinese hospitalized patients with SLE. The drug resistance of GNB has increased significantly. It was necessary to rational use of antibiotics in patients with SLE. <h3>Disclosure of Interest</h3> None declared
Tenascin-C and Integrin α9 Mediate Interactions of Prostate Cancer with the Bone Microenvironment
Authors: Rebeca San Martin, Ravi Pathak, Antrix Jain, Sung Yun Jung, Susan G. Hilsenbeck, María C. Piña-Barba, Andrew G. Sikora, Kenneth J. Pienta, David R. Rowley
Journal: Cancer Research (2017)

Abstract Deposition of the extracellular matrix protein tenascin-C is part of the reactive stroma response, which has a critical role in prostate cancer progression. Here, we report that tenascin C is expressed in the bone endosteum and is associated with formation of prostate bone metastases. Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin C exhibited enhanced adhesion and colony formation as mediated by integrin α9β1. In addition, metastatic cells preferentially migrated and colonized tenascin-C–coated trabecular bone xenografts in a novel system that employed chorioallantoic membranes of fertilized chicken eggs as host. Overall, our studies deepen knowledge about reactive stroma responses in the bone endosteum that accompany prostate cancer metastasis to trabecular bone, with potential implications to therapeutically target this process in patients. Cancer Res; 77(21); 5977–88. ©2017 AACR.
AB0824 The method of calculating the probability of osteopenia in patients with multifocal atherosclerosis
Authors: AN Kokov, VL Masenko, SE Semenov, TA Raskina, OL Barbarash
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Objectives</h3> To determine probability of the presence of osteopenic syndrome in patients with multifocal atherosclerosis based on the multislice computed tomography (MSCT) evaluation of vascular calcification. <h3>Methods</h3> 186 male (60±6,7 years) with multifocal atherosclerosis. All the patients underwent the measurement of BMD with X-ray absorptiometry. Moreover, calcium scores (CS) of coronary and brachiocephalic arteries were obtained using Agatston method. <h3>Results</h3> Among the patients had a high prevalence of osteopenia syndrome (87.1%). According to X-ray absorptiometry T-score values of lumbar vertebrae -1.07 [-1.54; -0.40], T-score of the proximal femur -2.01 [-2.71; -1.49]. We also found a large amount of calcification of the coronary arteries according MSCT: calcium score (CS) 471.8 [118.2; 916,8]. Calcification of the carotid arteries in patients of the study group was less pronounced: CS 113.9 [44.5; 300.8], but noted significant direct relationship between the degree of calcification of different vascular beds (r=0,35, p&lt;0,05). We have data on the significant inverse association between bone density and a coronary artery calcification (r=-0,29, p&lt;0,05), and the carotid artery (r=-0,22, p&lt;0,05) by using Spearman rank correlations. Factors that affect the probability of osteopenic syndrome (according X-ray absorptiometry) in patients with known rates of calcification of the coronary and carotid arteries were obtained by regression analysis. These factors were coronary CS (p=0.012), carotid CS (p=0.034), the mass of calcifications of the carotid arteries (p=0.025) and the presence of a stenosis of the carotid arteries (p=0.026). The predictive model for estimating the probability of the presence of osteopenia in patients with multifocal atherosclerosis has been is designed using regression coefficients of each of the factors. As a result, ROC-analysis of the area under the ROC-curve for this prediction model was 0.792 (p=0.0001). The model was tested in the study sample. The specificity of the model was 72.1%, sensitivity of the model was 80.2%. <h3>Conclusions</h3> The study results suggest that indicators of calcification of the carotid and coronary arteries resulting from a routine examination by MSCT of patients with multifocal atherosclerosis have a high predictive capacity for assessing the probability of the presence of osteopenic syndrome in this category of patients. <h3>Disclosure of Interest</h3> None declared
AB0163 Mesenchymal stem cells induce CD1C+ tolerogenic dendritic cells in human systemic lupus erythematosus via up-regulating FLT-3 ligand
Authors: X Yuan, D Wang, Z Zhang, Q Wang, W Li, X Tang, W Chen, G Yao, L Sun
Journal: Annals of the Rheumatic Diseases (2017)

<h3>Background</h3> CD1c<sup>+</sup> tolerogenic dendritic cells (DCs) play important roles in the induction of peripheral tolerance and control of adaptive immune response. Umbilical cord (UC)-derived mesenchymal stem cells (MSCs) exhibit immunoregulation effects in systemic lupus erythematosus (SLE). However, the underlying immunosuppression mechanism of MSCs via tolerogenic DCs in SLE remains largely unknown. <h3>Objectives</h3> The aim of this study was to examine tolerogenic DCs levels in SLE patients, and to further investigate the mechanism of MSCs in the regulation of tolerogenic DCs. <h3>Methods</h3> Tolerogenic DCs were isolated as Lin (CD3/19/56/14)<sup>-</sup> HLA DR<sup>+</sup>CD11c<sup>+</sup>CD1c<sup>+</sup> from peripheral blood mononuclear cells (PBMCs). Levels of tolerogenic DCs were determined by flow cytometry, and serum concentration of Flt-3 ligand (FLT3L) were determined by ELISA from 17 healthy controls and 25 SLE patients. Eight SLE patients were given UC MSCs infusions. We compared the levels of tolerogenic DCs and serum FLT3L before and 24 hours after UC MSCs transplantation. PBMCs from 8 patients were collected and co-cultured with UC MSCs at ratios of 1:1, 10:1 and 50:1, for 24 hours, 48 hours and 72 hours, respectively, to detect the levels of tolerogenic DCs. The FLT3L in the supernatant solution were determined. FLT3L siRNA was added to the co-culture system, and the level of tolerogenic DCs were detected. <h3>Results</h3> The levels of peripheral CD1c<sup>+</sup> DCs and serum FLT3L were significantly decreased in SLE patients compared to healthy controls. After UC MSCs transplantation, the levels of CD1c<sup>+</sup> DCs increased, along with an increase in serum FLT3L. In vitro studies showed that UC MSCs time-dependently up-regulated peripheral CD1c<sup>+</sup> DCs, but not dose-dependently. The supernatant FLT3L level significantly increased after co-cultured with MSCs. However, the addition of FLT3L siRNA significantly abrogated the up-regulation of CD1c<sup>+</sup> DCs by MSCs. <h3>Conclusions</h3> UC MSCs induce CD1c<sup>+</sup> tolerogenic DCs through up-regulating FLT3L in lupus patients. <h3>Disclosure of Interest</h3> None declared