Abstract LB-320: Evaluating changes in circulating tumor cell count in prostate cancer patients during androgen deprivation plus chemotherapy by using AxonDx enumeration device
Authors: Robert J. Amato, Reynolds Brobey, Yue Wang, Mehdi Dehghani, Zoe Tramel, Kent Murphy, Jeff Smith
Journal: Cancer Research (2018)

Abstract Background: Circulating tumor cell (CTC) counts have clinical utility as a prognostic marker in cancer prostate cancer (PCa). Using the FDA-approved CellSearch system, CTC counts &amp;gt5 CTC/7.5 ml convey a negative prognosis with respect to overall survival. The significance of sequential CTC counts during treatment is not fully understood. To determine whether longitudinal CTC counts can be used as a prognostic and/or predictive marker of clinical outcome in PCa patients undergoing androgen deprivation therapy (ADT) plus chemotherapy, we used the AxonDx nCyte non-enrichment-based CTC detection and enumeration system to evaluate CTC counts at baseline, during, and after therapy in 16 patients for up to 11 months. Methods: Whole blood was drawn from PCa patients participating in a clinical trial of ADT plus chemotherapy. CTCs were determined at multiple time points: at PSA evaluation (every 8 wks, ±radiographic reevaluation [every 16 wks]). Blood was collected into vacutainers containing K3-EDTA and processed within 6 hr. PBMC containing nucleated cells harvested from 6 ml of whole blood were fixed, permeabilized and blocked with BSA buffer, and stained using a proprietary antibody cocktail containing DAPI, an anti-cytokeratin mixture, the pan-leukocyte marker CD45, and markers selective for granulocytes and lymphocytes. Stained cells were drawn onto 1.2 µm pore filters, washed, placed on counting slides, and analyzed. CTCs are identified as cytokeratin-positive, nucleus-positive and leukocyte marker-negative. Results: CTCs were obtained from 12 patients for 2-9 months at 2-7 time points; from 2 patients for 11 months at 5-7 time points, and from 2 patients for 1 month at 1 time point. We detected time-dependent changes in CTC counts following treatment that did not necessarily correlate with patient's local prostate specific antigen (PSA) levels but were early indicators of subsequent increase in tumor burden. Conclusions: The AxonDx nCyte system can reliably detect CTCs from PCa patient samples that trend along with other signs of disease progression. CTCs can be used longitudinally for clinical monitoring of patients with PCa prior to signs of progression via tumor markers or scans during ADT plus chemotherapy. To further evaluate sequential CTC counts as a reliable biomarker for PCa, we plan additional studies to confirm our data and compare the AxonDx nCyte system with the CellSearch device in our ongoing clinical trial PCa program. Citation Format: Robert J. Amato, Reynolds Brobey, Yue Wang, Mehdi Dehghani, Zoe Tramel, Kent Murphy, Jeff Smith. Evaluating changes in circulating tumor cell count in prostate cancer patients during androgen deprivation plus chemotherapy by using AxonDx enumeration device [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr LB-320.