tbuckley/cabot-search huggingface dataset:
Abstract 12479: Class I and II Histone Deacetylases Orchestrate Muscle Regeneration Program Following Hind-limb Ischemia
Authors: Claudia Colussi, Francesco Spallotta, Simona Nanni, Jessica D Rosati, Silvia Tardivo, Stefania Straino, Antonello Mai, Maurizio C Capogrossi, Antonella Farsetti, Carlo Gaetano
Journal: Circulation (2012)

INTRODUCTION: Although a regulatory and functional cross-talk between class I and II HDACs have been hypothesized, the role of these molecules in the context of muscle regeneration following acute hind-limb ischemia has never been addressed and represents the object of this research.  METHODS & RESULTS: mice (C57BLJ10) were made ischemic by surgical excision of femoral artery and treated either with the selective class IIa inhibitor MC1568 (40 mg/Kg/day; n=12), the selective class I inhibitor MS275 (5 mg/Kg/day; n=12) or an equivalent amount of solvent (DMSO; n=12). Untreated ischemic (n=12) or sham operated animals (n=12) were used as further controls. Histological analyses revealed, from 3 to 7 days after damage, the prevalent localization of class IIa HDAC4 and 5 outside the nucleus. Following damage, dystrophin and nNOS proteins were down-regulated and decreased phosphatase PP2A activity was observed. Between 14 and 21 days after ischemia dystrophin and nNOS expression was reconstituted and paralleled by HDAC4 and 5 relocalization to the nucleus. Remarkably, the class IIa inhibitor MC1568 delayed muscle regeneration and immature fibers could be still detected 21 days after damage compared to ischemic untreated animals, while the class I selective inhibitor MS275 totally prevented postischemia muscle regeneration determining muscle atrophy, inflammation and fibrosis suggesting that members of different HDAC classes could be involved at different postischemia regeneration stages. Chromatin immunoprecipitation (ChIp) experiments demonstrated that A) during muscle regeneration class IIa HDACs bind and repress the promoter regions of proliferation-associated genes such as c-fos, telomerase, cyclin A1 and B1; B) class IIa HDACs bind class I HDAC2 promoter repressing its expression in late regeneration phases (>14 days post-ischemia). Accordingly, in MC1568-treated ischemic mice HDAC2 protein levels were increased as a consequence of class IIa inactivation.  CONCLUSION: These findings indicate a) opposite to class I nuclear localization of class IIa HDACs is important during late muscle regeneration after hind-limb ischemia; b) this molecular switch controls gene expression providing a functional link between different classes of HDACs.
Doctor whose scrubs emitted smell of alcohol is cleared of drink driving
Authors: Ted Davenport
Journal: BMJ (2017)

A hospital doctor has been cleared of drink driving after a court heard that a strong smell of alcohol came from cleaning fluid on his scrubs rather than wine.

David Burton was arrested three days after Christmas 2015, shortly after he had completed four consecutive night shifts at North Devon District Hospital in Barnstaple.

Staff at a shop …
Subclinical sensitization with diphenylcyclopropenone is sufficient for the treatment of alopecia areata: Retrospective analysis of 159 cases
Authors: Sung Jay Choe, Solam Lee, Long Quan Pi, Dong In Keum, Chung Hyeok Lee, Beom Jun Kim, Won-Soo Lee
Journal: Journal of the American Academy of Dermatology (2017)
A Cornucopia of Therapies under Study for Hemophilia
Authors: Glenn F. Pierce, Steven W. Pipe
Journal: Molecular Therapy (2017)

Hemophilia has historically been undiagnosed and untreated. Effective treatment began in the 1960s, first with the discovery that cryoprecipitate was enriched in factor VIII, followed subsequently by plasma fractionation processes that led to purified factor VIII and factor IX from pooled plasma. This ushered in a great inflection point in treatment, allowing effective bleed treatment, prevention, and home-based care. Mortality was reduced, and reduced morbidity from joint disease was realized. However, purified factor VIII and IX concentrates required the pooling of up to 120,000 donors, resulting in widespread viral contamination of plasma-derived clotting factors.
GPs are working beyond their competency to provide transgender care, BMA warns
Authors: Abi Rimmer
Journal: BMJ (2017)

GPs are putting themselves and patients at risk by acting beyond their competency to provide care for transgender patients, the BMA has said.

In response to an NHS England consultation on changes to gender identity services for adults, the BMA warned that GPs were being asked to prescribe specialist drugs that were beyond their expertise.1

It said, “We are concerned both about the safety of patients who are prescribed drugs off licence by a non-specialist prescriber, where there is a potential for harm to the patient and consequent medicolegal risk to GPs.”

Robert Morley, executive secretary of …
TCT-89 Tradeoff between Myocardial Infarction and Bleeding in Relation to Bleeding Risk: insights from PARIS registry
Authors: Sabato Sorrentino, Clayton Snyder, Usman Baber, Gennaro Giustino, Samantha Sartori, Serdar Farhan, Birgit Vogel, Jaya Chandrasekhar, Zhen Ge, Hannah Levine, David Cohen, Cono Ariti, C. Michael Gibson, Mitchell Krucoff, David Moliterno, Alaide Chieffo, Timothy Henry, Ioannis Iakovou, Antonio Colombo, Annapoorna Kini, Bernhard Witzenbichler, Philippe Gabriel Steg, Stuart Pocock, Roxana Mehran
Journal: Journal of the American College of Cardiology (2017)
Abstract 15108: Patient Centered Approaches to Research Enrollment (P-CARE)
Authors: Victoria M Scicluna, James C Blankenship, Chandan M Devireddy, Michael Frankel, Sara F Goldkind, Gautam Kumar, Qi Long, Andrea R Mitchell, Raul G Nogueira, Samantha Noreen, Ruth M Parker, Manesh R Patel, Rebecca D Pentz, Michele Riedford, Robert Silbergleit, Candace Speight, Kevin P Weinfurt, Neal W Dickert
Journal: Circulation (2016)

Introduction: Emergent informed consent for clinical trials in acute myocardial infarction (MI) and stroke is complicated by time pressure, severe illness, and stress. Insufficient data exist regar...