tbuckley/cabot-search huggingface dataset:
Identification of a novel gene product: Resveratrol-Induced Nuclear Protein (RINP)
Authors: Mohammad R. Salabat, Xiang Z. Ding, Michael B. Ujiki, Laleh Golkar, David J. Bentrem, Mark S. Talamonti, Richard H. Bell, Thomas E. Adrian
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

3254 

Background and Aim: Resveratrol is an antioxidant found in grape skins and other natural food products. Many studies have shown that resveratrol has anti-cancer properties. We have reported that Resveratrol significantly inhibits pancreatic cancer cell proliferation and induces apoptosis. The current study is aimed to To elucidate underlying molecular mechanisms for anti-cancer properties of resveratrol. Methods and results: An oligonucleotide microarray analysis was used to identify genes modified by resveratrol in CD18 pancreatic cancer cell line. Among multiple genes that are up- or down-regulated, we found an uncharacterized novel gene is significantly upregulated by Resveratrol, which is further confirmed by real time RT-PCR. With 5-RACE and 3-RACE (Rapid Amplification of cDNA End) PCR based on the EST sequence of the novel gene, a full length cDNA of the novel gene is cloned out. The cDNA is 1879 bp long containing a 615 bp open reading frame, which encodes a protein with 205 amino acid residues. Computational analysis using PSORT II online software suggests that the novel gene product contains a putative single strand DNA binding domain. Therefore, we named this novel gene product Resveratrol-induced DNA binding protein (RINP). By fusing ORF of the novel gene with V5 epitope sequence in a pLenti 6/V5 expression vector, we studied the localization of RINP by immunofluoresence staining and confocal microscope. We found that full length RINP is localized in the nucleus. Since both the N and C terminals of RINP are heavily basic and thus are possible sites for a nuclear localization signal, therefore, we established two truncated RINP expression vectors containing only either N-terminal or C-terminal RINP alone. We found that either N-terminal or C-terminal RINP alone is enough to signal the novel protein to nucleus. We also examined distribution of RINP in multiple normal human tissues using real time RT-PCR. We found that RINP is expressed in every tissue tested with highest expression in liver and placenta and the lowest in brain. Conclusion: We have characterized a novel gene induced by Resveratrol in pancreatic cancer cells, CD18 cell line, which contains a putative single strand DNA binding site and is localized to the nucleus. Further studies are undergoing to identify the function of this gene with a focus on its role in resveratrol-induced growth inhibition and apoptosis in cancer cells.
In MDS Targeted Deep Sequencing of CD34+ Blood Cells but Not Peripheral Blood Mononuclear Cells Give Results Which Are Representative for Bone Marrow
Authors: Roman Martin, Laura Palomo, Christina Ganster, Pamela Acha, Sascha Dierks, Mar Mallo, Vera Adema, Francisco Fuster-Tormo, Paula Gomez-Marzo, Nuria De Haro, Neus Solanes, Lurdes Zamora, Blanca Xicoy, Arkadiusz Kominowski, Martin Stromburg, Alina Brockmann, Lorenz H. Truemper, Francesc Sole, Detlef Haase
Journal: Blood (2016)
Роль гендерного диморфізму у розвитку та наслідках хронічних вірусних гепатитів
Authors: V.B. Yagmur
Journal: GASTROENTEROLOGY (2017)

Хронічні захворювання печінки вірусного походження до цього часу займають провідне місце в структурі смертності від гепатобіліарної патології та причин транс­плантації печінки. Пошук факторів, що впливають на прогресування фіброзу і прогноз захворювання, є актуальним завданням охорони здоров’я. У статті наводиться огляд досліджень впливу статевих гормонів на прогресування фіброзу печінки, імовірність розвитку гепатоцелюлярної карциноми у чоловіків і жінок репродуктивного віку і в періоді менопаузи.
Higher risk-taking behavioursbehaviors and sensation seeking needs in collegiate student-athletes with a history of multiple sport-related concussions
Authors: Erica Beidler, Tracey Covassin, MB Donnellan, Sally Nogle, Matthew B Pontifex, Anthony P Kontos
Journal: British Journal of Sports Medicine (2017)

Objective To investigate differences in risk-taking behaviours and sensation seeking needs between collegiate student-athletes with and without a history of sport-related concussion (SRC). Design Cross-sectional quantitative study. Setting Four National Collegiate Athletic Association (NCAA) universities. Participants A total of 1,398 (68%) of 2,055 collegiate student-athletes completed the survey. There were 146 participants excluded due to: age&lt;18, current SRC/acute musculoskeletal injury, SRC within 3-months, non-NCAA, non-SRC, and incomplete survey. Therefore, 1,252 subjects from 18 sports were included. Assessment of risk factors A10-minute survey was administered that included demographic information, SRC history, Barratt Impulsiveness Scale (BIS) for risk-taking, and Brief Sensation Seeking Scale (BSSS). The independent variable was SRC history: 0(n=938), 1(n=205), 2+(n=109). Outcome measures The dependent variables were BIS total risk-taking impulsivity and BSSS total sensation seeking scores. Main results Significant differences were found between SRC groups for the totalrisk-taking impulsivity [F(1,227)=7.15,p=0.00] and sensation seeking [F(1,214)=4.53,p=0.01] variables. Total risk-taking impulsivity was significantly higher for the 2+ SRC group compared to the 0 SRC group (MD±95%CI=0.18 ± 0.11,p=0.00) and the 1 SRC group (MD±95%CI=0.15 ± 0.12,p=0.02). Total sensation seeking scores were significantly higher for the 2+ SRC group compared to the 0 SRC group (MD±95%CI=0.19 ± 0.17, p=0.03). Conclusions Total risk-taking and sensation seeking scores were higher for collegiate student-athletes with a history of multiple SRC. This finding could be useful when implementing behaviour modifications for safer play. Future longitudinal research should adopt a pre-/post-test design to determine if increased risk-taking and sensation seeking are SRC risk-factors or if multiple SRCs cause an increase in psychological variables. Competing interests None.
Postirradiation Lichen Sclerosus et Atrophicus
Authors: Kathleen M. Nemer, Milan J. Anadkat
Journal: JAMA Dermatology (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Dermatology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Dermatology journal
Résection “Full Thickness” per endoscopique de la paroi colique dans la prise en charge des résidus adénomateux après mucosectomie: Première série pilote Française de 5 patients
Authors: Y Le Baleur, E Anouk, G Charlotte, M Levy, A Amiot, F Venezia, F Brunetti, D Nicolas, M Farida, I Sobhani
Journal: Endoscopy (2016)

La prise en charge d'une récidive adénomateuse après mucosectomie colique est un problème thérapeutique complexe car la fibrose sous muqueuse cicatricielle rend difficile voir impossible les résections endoscopiques ultérieures conduisant le plus souvent à une chirurgie d'exérèse par colectomie dont la morbi -mortalité bien que faible n'est pas nulle. La résection colique transpariétale per endoscopique dite «Full Thickness» pourrait permettre d'éviter un recours systématique à la chirurgie après échec des techniques de résection endoscopiques conventionnelles.
Perception and practice of prescribed rest following concussion in primary and secondary schools
Authors: Alex M Taylor, Laura S Blackwell, Anna F Robinson, Mark R Proctor
Journal: British Journal of Sports Medicine (2017)

Objective The purpose of our study was to characterise return-to-learn practices following concussion in primary and secondary schools, particularly with regard to prescribed cognitive rest. Design Cross-sectional survey. Setting Massachusetts. Participants Nine hundred member of the Massachusetts School Nurse Research Network (MASNRN) employed full or part-time at a public, private, or charter school serving pre-school through the 12th grade. Outcome measures Prescribed rest (actual) versus perceived benefit of rest. Main results A majority of respondents expressed the opinion that more prolonged cognitive rest benefits recovery, with 53.6% endorsing 4–10 days as beneficial compared to 44.3% endorsing 3 days or less. Only 2.1% felt no benefit to cognitive rest following concussion. The average number of medically advised rest (actual) was equal for older and younger students (4.5 days). School nurses perceived less benefit to prolonged cognitive rest (&gt;4 days) for high school students relative to students in elementary school [2 (4, n=97)=11.05, p&lt;0.05], but provided academic accommodations to high school students for comparatively longer periods of time (10–14 days vs. 6–10 days) [F(2,89)=4.74, p=0.011]. Conclusions Counter to the practice of administering accommodations for fewer days in younger students, school nurses perceived greater benefit to shorter periods of cognitive rest (3 days or less) for older children (high school age) relative to elementary school and middle school children. In all settings, schools rest is overprescribed in light of recent finding indicating that prolonged rest (beyond two to three days) may delay recovery. Competing interests None.
Abstract 282: Chemically Tailoring Cardiovascular Drugs for Alzheimer's Disease Drug Discovery
Authors: Giulio Pasinetti, Lap Ho, Kenjiro Ono, Jun Wang
Journal: Circulation Research (2012)

We previously reported that carvedilol, a brain bioavailable and bioactive antihypertensive agent, significantly reduces brain contents of Alzheimer’s disease (AD)-type β-amyloid (Aβ) oligomers experimentally. Currently, we are exploring clinical applications of carvedilol to prevent or attenuate the progression of AD dementia. However, the potential development of carvedilol for AD is complicated by its cardiovascular activities, including bradycardia and hypotension, particularly among patients already undergoing treatment with other cardiovascular medications. Based on this, using structure-activity relationship (SAR) medicinal chemistry we are developing novel modified carvedilol lead compounds with decreased cardiovascular features for treating AD. Testing commercially available compounds identified from a preliminary screening of a library of carvedilol analogs, we identified 6 bioactive small-molecule compounds that significantly reduce Aβ oligomerization in vitro. Anti-Aβ oligomerization activity was confirmed by circular diachroism and electron microscopy. One compound, (R)-(+)-4-hydroxyphenyl carvedilol, showed improved anti-oligomerization activity and reduced α-1 adrenergic receptor binding activity compared to carvedilol. Short-term proof-of-concept in vivo testing showed that treatment with 1.5 mg/kg/day (R)-(+)-4-hydroxyphenyl carvedilol significantly reduced levels of oligomeric Aβ and total Aβ in the brains of a transgenic mouse model of AD-type Aβ neuropathology without cardiovascular complications. We are currently evaluating the effects of (R)-(+)-4-hydroxyphenyl carvedilol on cardiovascular functions and the attenuation of Aβ-mediated cognitive dysfunction in experimental AD models. Our study provides the critical basis for exploring the bioavailability and pharmacokinetic characteristics of novel cardiovascular lead compounds with enhanced brain activities and reduced adverse events in IND-directed pre-clinical safety assessments and eventually Phase I clinical applications in AD.
Cancer Death Rates Decrease in Men, Women, and Children
Authors: Jennifer Abbasi
Journal: JAMA (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Prognostic Value of Baseline Quantitative PET Metrics for Patients with Unfavourable Early Stage Hodgkin Lymphoma Enrolled in the Standard Arm of the EORTC/Lysa/FIL H10 Trial
Authors: Anne Ségolène Cottereau, Annibale Versari, Annika Loft, Rene-Olivier Casasnovas, Monica Bellei, Romain Ricci, Stephane Bardet, Antonio Castagnoli, Pauline Brice, John Raemaeker, Emmanuel Itti, Catherine Forpied, Tiana Raveloarivahy, Theodore Girinsky, Thierry Van der Borght, Massimo Federico, Martin Hutchings, Umberto Ricardi, Michel Meignan, Marc Andre
Journal: Blood (2016)
Effect of a history of 3+ previous concussions on centre of pressure variables during quiet stance
Authors: Jill K Dierijck, Alexander D Wright, Kelsey Bryk, Jonathan D Smirl, Paul van Donkelaar
Journal: British Journal of Sports Medicine (2017)

Objective To determine the influence of a history of 3+ previous concussions on quiet stance. Design Retrospective cohort. Setting Laboratory. Participants Eighty-seven male (age range 17–22) contact-sport athletes (football and hockey) entered the study. Participants were recruited from the same sport teams and tested during the pre-season. Exclusion criteria: concussion in the prior 6 months, or history of 1 or 2 concussions. Forty-four met the inclusion criteria: thirty-one had no previous concussions, and thirteen had a history of 3+ concussions. Interventions Participants held quiet upright stance on a force plate (NDI True Impulse) with feet hip-width apart and hands on hips with eyes-open (1 min), and closed (1 min). Independent variables included condition (2) and concussion history (2). Outcome measures Root-mean-square displacement (RMSd), and mean velocity of centre-of-pressure (COP) in the anterior/posterior (AP) and medial/lateral (ML) directions. Main results Mixed ANOVA indicated a significant condition effect in ML-RMSd (p=0.015, 95% CI: 0.032–0.277) between eyes-open and eyes-closed conditions. ML mean velocity also displayed a significant condition effect (p=0.025, 95% CI: 0.025–0.358). In contrast to the effect of visual input, there was no main effect of concussion history on these metrics. Conclusions A history of 3+ concussions does not appear to result in long-term deficits controlling in quiet stance. Those ML variables were sensitive to condition and indicate that frontal plane COP movements may be more sensitive than those in the sagittal plane. These findings are important as a history of concussion does not appear to alter quiet stance balance control mechanisms. Competing interests None.
Haptic Feedback in Needle Insertion Modeling and Simulation
Authors: Gourishetti Ravali, Muniyandi Manivannan
Journal: IEEE Reviews in Biomedical Engineering (2017)

Needle insertion is the most basic skill in medical care, and training has to be imparted not only for physicians but also for nurses and paramedics. In most needle insertion procedures, haptic feedback from the needle is the main stimulus in which novices need training. For better patient safety, the classical methods of training the haptic skills have to be replaced with simulators based on new robotic and graphics technologies. This paper reviews the current advances in needle insertion modeling, classified into three sections: needle insertion models, tissue deformation models, and needle-tissue interaction models. Although understated in the literature, the classical and dynamic friction models, which are critical for needle insertion modeling, are also discussed. The experimental setup or the needle simulators that have been developed to validate the models are described. The need of psychophysics for needle simulators and psychophysical parameter analysis of human perception in needle insertion are discussed, which are completely ignored in the literature.
BRIE: transcriptome-wide splicing quantification in single cells
Authors: Yuanhua Huang, Guido Sanguinetti
Journal: Genome biology (2017)

Single-cell RNA-seq (scRNA-seq) provides a comprehensive measurement of stochasticity in transcription, but the limitations of the technology have prevented its application to dissect variability in RNA processing events such as splicing. Here, we present BRIE (Bayesian regression for isoform estimation), a Bayesian hierarchical model that resolves these problems by learning an informative prior distribution from sequence features. We show that BRIE yields reproducible estimates of exon inclusion ratios in single cells and provides an effective tool for differential isoform quantification between scRNA-seq data sets. BRIE, therefore, expands the scope of scRNA-seq experiments to probe the stochasticity of RNA processing.
Abstract #2298: High throughput screening identifies two classes of antibiotics: tetracycline and fluoroquinolone eliciting radioprotection
Authors: Kwanghee Kim, Julianne Pollard, Andrew Norris, J. McDonald, Ewa Micewicz, Robert Damoiseaux, Kei Iwamoto, James Sayre, Richard Gatti, William McBride
Journal: Cancer Research (2009)

Whole body irradiation causes severe apoptotic cell death of radiation sensitive tissues. Among them, acute hematopoietic syndromes are serious problem even at low dose exposure. Thus, agents protecting immune system from radiation cytotoxicity would be immediately useful for the case of radiation emergencies. From a high throughput screening of over 33,000 compounds of small molecule libraries, two classes of antibiotics, tetracycline analogs and fluoroquinolones, clearly emerged as being radioprotective towards a mouse lymphocyte cell line. These compounds also protected human lymphoblastoid cells against radiation-induced apoptosis and were further tested in vitro and in vivo. In vivo, two injections of tetracycline prior to a lethal dose of radiation to the whole body (8 Gy) improved survival to 87.5% while all vehicle treated mice expired. The enhanced survival might be due to the protection of both lymphoid and myeloid stem/progenitor populations from radiation damage. Tetracycline injection post 8 Gy irradiation also improved the survival, but with lesser efficacy. In vitro pretreatment of human lymphoblastoid cells with tetracycline at 20 \#956;M reduced the foci formation of histone H2AX, indicating the protection from DNA double strand breaks. In addition, tetracycline does not interfere with radiotherapy of mouse lung tumor cells grafted in mice suggesting potential application for cancer radiotherapy. The mechanisms of tetracycline action in both normal tissue protection and sensitization of tumor cells are currently being investigated. This work was supported by UCLA\#8217;s Center for Biological Radioprotectors U19 AI067769/NIAID.   Citation Information:  In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 2298.
Costs and contexts: factors affecting self-report of concussion in a military academy
Authors: Christopher D’Lauro, Brian Rudolph Johnson, Craig A Foster, Gerald McGinty, Darren Campbell
Journal: British Journal of Sports Medicine (2017)

Objective To understand the factors affecting the decision to self-report a concussion – particularly among sub-groups like athletes and future pilots. Design Survey. Setting A United States military academy. Participants Voluntarily participatingcadets (n=2,503, 23.9%=female). Assessment of risk factors NCAA Division I athlete status, future pilot status, gender, school year were examined as factors affecting self-report. Outcome measure Anticipated Self-Report (ASR) of concussion on a scale from 1–9 (most to least likely to self-report). Cadets completed a 2-page survey matching ASR to demographic risk factors (above), and to self-report questions assessing Costs, Rewards, Attitude, Subjective Norms, Self-Efficacy, and Social Support. Main results Cadets demonstrated an overall willingness to self-report a concussion – indicated by a mean ASR score of 6.07 (95% CI=5.97–6.17, SD=2.47, median=7.00, n=2.332) above the scale midpoint. Costs (r=−0.61, p&lt;0.001), Rewards (r=0.67, p&lt;0.001) and Attitude (r=0.70, p&lt;0.001) were highly, significantly predictive of ASR. Cadet sub-populations revealed the importance of specific costs. Aspiring pilots – concerned about concussions affecting their pilot qualification status –steadily decreased ASR by year, while non-pilot cadets retained consistent ASR, shown by Future Pilot x Class Year ANOVA ( F (3,2196)=11.78, p&lt;0.001). NCAA Athletes, conversely, showed no ASR differences from non-athletes, t(2251)=0.16, p=0.87, CI =±0.23. Conclusions Symptom self-report is a crucial factor in all stages of concussion care. Costs and rewards affecting self-report may have unique contexts that drive unexpected self-report patterns. Here, aspiring pilots showed lower self-report behaviour while athletes showed average self-report behaviour – based on perceived costs to career aspirations. These data will be used to design interventions to increase to concussion self-report. Competing interests All authors received funding from the National Collegiate Athletic Association and Department of Defense (NCAA Mind Matters Challenge).
Causal evidence for retina-dependent and -independent visual motion computations in mouse cortex
Authors: Daniel Hillier, Michele Fiscella, Antonia Drinnenberg, Stuart Trenholm, Santiago B Rompani, Zoltan Raics, Gergely Katona, Josephine Juettner, Andreas Hierlemann, Balazs Rozsa, Botond Roska
Journal: Nature Neuroscience (2017)