tbuckley/cabot-search huggingface dataset:
Abstract 117: MicroRNA-138 regulates S100A1 in Endothelial Cells
Authors: Anagha Sen, Shumei Ren, Jianxin Sun, Patrick Most, Karsten Peppel
Journal: Circulation Research (2013)

Rationale: The EF-hand Ca2+ sensor S100A1 is essential for proper endothelial nitric oxide (NO) synthase (eNOS) activation. S100A1 levels are greatly reduced in endothelial cells (ECs) subjected to hypoxia, rendering them dysfunctional. Objective: To determine if the 3’UTR mediates the rapid hypoxia-induced downregulation of S100A1 in ECs. Methods and Results: ECs transfected with a S100A1 - 3’ untranslated region (UTR) luciferase reporter construct displayed significantly reduced gene expression when subjected to gas or chemical hypoxia. Bioinformatic analysis suggested that microRNA -138 (miR-138) could target the 3’UTR of S100A1. Hypoxia greatly increased miR-138 levels in ECs, but not in skeletal muscle C2C12 myotubes. Consistent with this finding, patients with critical limb ischemia (CLI) or mice subjected to femoral artery resection (FAR) displayed increased miR-138 levels. Transfection of a miR-138 mimic into ECs reduced S100A1 - 3 ‘UTR reporter gene expression, while transfection of an anti miR-138 (antagomir) prevented the hypoxia-induced downregulation of the reporter gene. The increased levels of miR-138 are dependent on Hif1-α activation as treatment with siRNA against Hif1-α prevented S100A1 reporter gene downregulation after hypoxia. Conversely, specific activation of Hif1-α by a selective prolyl-hydroxylase inhibitor (IOX2) reduced reporter gene expression. Finally, ECs transfected with miR-138 mimic displayed reduced tube formation when plated onto Matrigel matrix and expressed less NO when stimulated with VEGF. These effects were reversed by gene transfer of S100A1 using recombinant adenovirus. Conclusions: Our study shows that miR-138 is an essential mediator of EC dysfunction via its ability to target the 3’UTR of S100A1 in a hypoxia-induced manner. MiR-138 might thus be an attractive target for the treatment of pathologies that are linked to endothelial dysfunction.
INCIDENCE AND RISK FACTORS OF INJURIES IN BRAZILIAN ELITE VOLLEYBALL PLAYERS: A PROSPECTIVE COHORT STUDY
Authors: Rafael M Pimenta, Luiz C Hespanhol Junior, João A Grangeiro Neto, Alexandre D Lopes
Journal: British Journal of Sports Medicine (2017)

Background Volleyball can be considered one of the most popular sports. Prospective studies aimed at investigating the incidence and risk factors of volleyball injuries are scarce. Objective To investigate the incidence and risk factors for volleyball injuries among Brazilian elite volleyball players. Design 20-week prospective cohort study. Setting Brazilian elite national league (2015–2016). Participants 13 Brazilian elite volleyball teams (208 players): 7 male teams (116 players) and 6 female teams (92 players). Assessment of Risk Factors Volleyball exposure (hours and sessions of training, strength training and number of matches) and injuries were recorded weekly by the medical team. Main Outcome Measurements Injury during the follow-up period. Results 196 injuries were reported among 96 athletes. The overall injury incidence was 4.8 injuries/1000 hours of training and 18.6 injuries/1000 matches. Overuse injuries (65.3%, n=128) were more common than acute injuries (33.7%, n=66). Knee (28.5%, n=50) and shoulder (9.8%, n=19) were the body regions most affected by overuse injuries. Ankle (6.2%, n=12) and shoulder (5.6%, n=11) were the body regions most affected by acute injuries. Tendinopathy (23.7%, n=46) was the overuse injury type most observed, and ankle sprain (7.2%, n=12) was the acute injury type most observed. Female athletes (OR: 0.50, CI 95%: 0.31–0.80) and additional training sessions (OR: 0.74, CI 95%: 0.60–0.91) were associated with a lower risk of injury. Previous injury (OR: 2.18, CI 95%: 1.40–3.38), and an additional hour of training (OR: 1.18, CI 95%: 1.05–1.32) were associated with a higher risk of injury. Conclusions This study showed that male gender, an additional hour of training, and a history of previous injury were the most significant risk factors for developing a new injury. However, additional training session was associated with lower considered risk of injury.
The atypical antipsychotic olanzapine causes weight gain by targeting serotonin receptor 2C
Authors: Caleb C. Lord, Steven C. Wyler, Rong Wan, Carlos M. Castorena, Newaz Ahmed, Dias Mathew, Syann Lee, Chen Liu, Joel K. Elmquist
Journal: Journal of Clinical Investigation (2017)

Atypical antipsychotics such as olanzapine often induce excessive weight gain and type 2 diabetes. However, the mechanisms underlying these drug-induced metabolic perturbations remain poorly understood. Here, we used an experimental model that reproduces olanzapine-induced hyperphagia and obesity in female C57BL/6 mice. We found that olanzapine treatment acutely increased food intake, impaired glucose tolerance, and altered physical activity and energy expenditure in mice. Furthermore, olanzapine-induced hyperphagia and weight gain were blunted in mice lacking the serotonin 2C receptor (HTR2C). Finally, we showed that treatment with the HTR2C-specific agonist lorcaserin suppressed olanzapine-induced hyperphagia and weight gain. Lorcaserin treatment also improved glucose tolerance in olanzapine-fed mice. Collectively, our studies suggest that olanzapine exerts some of its untoward metabolic effects via antagonism of HTR2C.
Computed Tomography Score of Aortic Valve Tissue May Predict Cerebral Embolism During Transcatheter Aortic Valve Implantation
Authors: Norio Tada, Yoshihiro Haga, Shinichi Suzuki, Yusuke Enta, Masaki Miyasaka, Hiroshi Inoue, Masataka Taguri, Kiyoshi Ishii, Masaki Hata, Masashi Sakuma, Shigeru Toyoda, Teruo Inoue, Tatsushi Ootomo
Journal: JACC. Cardiovascular imaging (2017)
Proceedings of the Fifth National Gastrointestinal Cancer Conference
Journal: JNCI Journal of the National Cancer Institute (1953)

Journal Article Proceedings of the Fifth National Gastrointestinal Cancer Conference Get access Morris K. Barrett, M. D. Morris K. Barrett, M. D. Executive Secretary Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 13, Issue 4, February 1953, Pages 927–1093, https://doi.org/10.1093/jnci/13.4.927 Published: 01 February 1953 Article history Received: 01 November 1952 Published: 01 February 1953
Morbidité de l’échoendoscopie interventionnelle: expérience d’un centre référent incluant 129 patients consécutifs
Authors: B Bournet, JL Bornet, M Delacroix, I Migueres, D Vigouroux, J Frexinos, J Escourrou, L Buscail
Journal: Endoscopy (2004)

Introduction: L’échoendoscopie (EE) a pris actuellement une place prépondérante dans la prise en charge des cancers digestifs et des affections bilio pancréatiques compte tenu de la possibilité d’effectuer des ponction biopsies dirigées, drainages de pseudo kystes et alcoolisation du plexus coeliaque. Nous avions précédemment évalué la morbidité de l’EE diagnostique à 0,93 ‰, la mortalité étant nulle. La présente étude expose la morbidité de l’EE interventionnelle évaluée de façon prospective dans un centre référent. Patients et Méthodes: De mars 2001 à septembre 2003, 133 EE interventionnelles ontété effectuées chez 129 patients consécutifs (70 hommes, 59 femmes, âge moyen 60 ans (25–80 ans)). Elles ont été réalisées par 1 même opérateur (appareils Olympus® GF-UC30P et Pentax® FG36UX). Il s’agissait de ponction biopsies (lésions pancréatiques tissulaires n = 53 ou kystiques pancréatiques n = 46, lésions extra digestives n = 27), de 5 alcoolisations du plexus coeliaque et de 2 ponction drainages de pseudokyste pancréatique. Les EE ont été réalisées sous anesthésie générale (Propofol) sans intubation programmée. Après l’examen, les patients ont tous eu une contre visite médicale (2 à 3 heures après la sortie de la salle de réveil) et ont été laissés à jeun au moins 6 heures. Tous les patients ont été hospitalisés au minimum 20 heures après le geste. Une antibioprophylaxie a été appliquée pour les ponctions de lésions kystiques, les lésions péri rectales et chez les porteurs de valves cardiaques. Résultats: Aucun décès n’a été déploré. Aucune intubation non programmée a été effectuée. Quatre complications sont survenues: une pancréatite aiguë après ponction biopsie d’une tumeur endocrine pancréatique (stade A de balthazar, délai d’apparition 7 heures, durée d’hospitalisation 3 jours); douleur abdominale et fièvre après cytoponction d’une tumeur kystique du pancréas (délai d’apparition 12 heures, hospitalisation 3 jours); perforation rétropéritonéale en regard de D3 après ponction biopsie d’une tumeur intracanalaire mucineuse papillaire pancréatique (délai d’apparition 8 heures, hospitalisation 5 jours); pleuro péricardite aiguë après ponction d’une masse médiastinale (délai d’apparition 48 heures, hospitalisation 21 jours). Il n’y a pas eu de séquelles à long terme de ces complications avec un suivi supérieur à 6 mois. Conclusion: L’EE interventionnelle possède une mortalité nulle et une morbidité de 3%. La réalisation par un opérateur confirmé, en dehors de l’urgence et avec une évaluation anesthésique préalable peut expliquer cette faible morbidité. Malgré cela, un délai moyen de survenue des complications supérieur à 6 heures, peut remettre en cause la réalisation de ce geste en ambulatoire comme cela a déjà été préconisé.
Abstract 144: Interstitial Formation of Adenosine and Inosine Is Proarrhythmic Through Adenosine A1 Receptor in the Developing Heart
Authors: Elodie Robin, Jessica Sabourin, Eric Raddatz
Journal: Circulation Research (2012)

We previously established that exogenous adenosine (ADO) induces transient arrhythmias in the developing heart via the ADO A1 receptor (A1AR) and downstream activation of NADPH oxidase/ERK and PLC/PKC pathways. We assessed the hypothesis that both endogenous ADO and its derived compound inosine (INO) can simultaneously act in an autocrine/paracrine manner and induce rhythm and conduction disturbances. The validated model of the spontaneously beating heart obtained from 4-day-old chick embryos was used. The basal ADO/INO ratio determined by HPLC was approximately 10. Blockade of Equilibrative Nucleoside Transporters (ENTs) by dipyridamole, which is known to increase interstitial ADO level, induced transient atrial dysrhythmias and atrio-ventricular blocks in 70% of the hearts (n=30) and, in parallel, increased ERK2 phosphorylation. When extracellular conversion of AMP to ADO by ecto-5’-nucleotidase/CD73 was inhibited by AOPCP, the dipyridamole-induced arrhythmias were prevented. The dipyridamole-induced arrhythmias and ERK2 phosphorylation were prevented by the specific antagonist of the A1AR (DPCPX) but not by antagonists of A2AAR ( SCH58261 ), A2BAR (MRS1754) or A3AR (MRS1523). Interestingly, inhibition by EHNA of the conversion of ADO to INO by adenosine deaminase was not proarrhythmic whereas exogenous INO (500 µM) induced arrhythmias which were prevented by DPCPX. In conclusion, these findings suggest that endogenous ADO and INO in the interstitial compartment of the developing heart can provoke transient pacemaking and conduction disturbances via A1AR and downstream ERK activation in an autocrine/paracrine manner. Overall, our results provide new insights into the mechanisms underlying cardiac dysfunction when a hypoxic-ischemic episode induces local overproduction of ADO and INO
Drainage »en un seul temps« des pseudo-kystes pancréatiques guidée par échoendoscopie. Résultats chez 36 patients
Authors: M Giovannini, E Bories, CH Pesenti, C Danisi
Journal: Endoscopy (2004)

But: Le but de cette étude a été d’évaluer une nouvelle méthode de drainage des pseudo kystes pancréatiques (PKP) en utilisant un système de drainage en un seul temps (Giovannini needle-wire (GNW), Wilson-Cook Company). Cet accessoire associe un infundibulotome, un cathéter dilatateur (6 ou 7 F), une prothèse plastique droite de 8,5 ou 10 F et un pousse prothèse. L’infundibulotome présente une partie interne rigide qui peut être retirée et ainsi devenir un fil guide souple pouvant être facilement enroulé dans le PKP. L’utilisation de ce système permettait de réduire le nombre de manipulation notamment d’échange du fil guide et du cathéter. Ce drainage était entièrement guidé par échoendoscopie en utilisant un échoendoscope interventionnel muni d’un canal opérateur de 3,2 ou 3,8 mm et d’un élévateur. Patients et Méthodes: De janvier 1999 à juin 2003, un drainage d’un PKP a été réalisé sous guidage échoendoscopique en utilisant le GNW, il s’agissait de 25 hommes et 11 femmes d’âge moyen 56,7 ans (extrêmes 29–69 ans). La taille moyenne des 36 lésions kystiques était de 5,8 cm (4–8 cm). Les PKP étaient localisés dans la tête du pancréas dans 6 cas, dans le corps dans 16 cas et au niveau de la queue dans 14 cas. Les échoendoscopes utilisés étaient le FG 38X et le EG 38UT fabriqués par les sociétés Pentax-Hitachi. Résultats: Aucune complication majeure n’est survenue notamment hémorragique. Une patiente a présenté un pneumopéritoine qui a nécessité un traitement chirurgical. Dans tous les cas, la prothèse kysto digestive a pu être mise en place. Il s’agissait 22 fois d’une prothèse de 8,5 F et 14 fois d’une prothèse de 10 F. Le choix de la taille était fonction du contenu du PKP. Nous avons noté 2 migrations de prothèses, une dans le PKP et la seconde dans le parenchyme pancréatique. Trois récidivessont survenues avec un suivi moyen de 29 mois (6–48 mois). Deux patients ont été opérés et l’autre a été traité de nouveau endoscopiquement avec succès. Conclusion: Le drainage »en un seul temps« guidé par échoendoscopie a été efficace chez 31/36 patients (86,1%).
Developing the Regulatory Utility of the Exposome: Mapping Exposures for Risk Assessment through Lifestage Exposome Snapshots (LEnS)
Authors: Rachel M. Shaffer, Marissa N. Smith, Elaine M. Faustman
Journal: Environmental Health Perspectives (2017)

Exposome-related efforts aim to document the totality of human exposures across the lifecourse. This field has advanced rapidly in recent years but lacks practical application to risk assessment, particularly for children's health.
Les traitements modifiant la fonction plaquettaire augmentent-ils le risque hémorragique après endoscopie thérapeutique? Une étude cas-témoins
Authors: N Hussain, A Barkun, P Burtin, Y Toubouti, E Rahme, JF Boivin
Journal: Endoscopy (2004)

Introduction: Il existe peu de données sur l’innocuité des gestes thérapeutiques endoscopiques chez les patients prenant des agents anti plaquettaires. Buts: évaluer si la prise d’agents anti-plaquettaires est associée à une augmentation du risque hémorragique après un traitement endoscopique. Patients et Méthodes: Une étude cas témoins a été réalisée à partir de cas de saignement non équivoque (selon des critères précédemment publiés) survenu après une polypectomie endoscopique du tube digestif supérieur ou inférieur, une sphinctérotomie endoscopique (SE) ou une gastrostomie perendoscopique dans un centre tertiaire ayant des endoscopistes expérimentés. Deux ou 3 patients témoins (sans hémorragie) ont été sélectionnés au hasard pour chaque cas par consultation d’une base de données administrative institutionnelle. L’appariement a été réalisésur le sexe, l’âge ± 15 ans, et le type de procédure chez les patients dont les données cliniques étaient disponibles. Un modèle de régression logistique conditionnelle a été utilisé pour rechercher une association entre l’exposition à un traitement anti-plaquettaire (dernière dose au cours des 10 derniers jours) et un saignement post-endoscopique en ajustant sur les facteurs de confusion potentiels connus. La puissance de l’étude a été fixée pour affirmer une association cliniquement significative. Résultats: Parmi les 44 cas, 50% ont saigné au cours de la procédure, 46% au cours des 14 jours suivants, et 4% plus tardivement. Cinquante pour cent de ceux qui ont saigné, ont nécessité une transfusion sanguine (moyenne: 1,5 ± 1,8 unités). Les 132 témoins et les 44 cas étaient correctement appariés pour l’âge (69 ± 16 vs 68 ± 17 ans), le sexe (H/F = 1/1 pour chaque groupe), le TP (normal chez 91 vs 82%), et la numération plaquettaire (> 60000 x 109/L chez tous). Des différences dans la répartition des angiocholites initiales (22% vs 48%), et de l’INR (> 1,5 dans 2% vs 9%) ont été observées. Au total, 20% des témoins et 23% des cas ont été exposés aux agents anti plaquettaires. Après ajustement sur les effets potentiellement liés une variation du TP et de l’INR, ainsi que sur l’existence d’une angiocholite dans le groupe SE (tous des facteurs de confusion), l’exposition à un agent anti plaquettaire n’était pas associée à un risque accru de saignement après traitement endoscopique (RR: 1,04; IC: 0,4–2,5). Conclusion: Cette étude cas témoins constitue une des rares études contrôlées disponibles suggérant que l’exposition à un agent anti-plaquettaire n’augmente pas le risque d’hémorragie après un traitement endoscopique de façon significative.
Inoueバルーンによる経皮的肺動脈弁形成術(PTPV)が著効した成人肺動脈弁狭窄症(PS)の一例
Authors: 廣岡慶治, 石津宜丸, 中川彰人, 小出雅雄, 岩破俊博, 篠田幸紀, 小向賢一, 山戸昌樹, 佐々木典子, 山元博義, 川口義廣, 是恒之宏, 楠岡英雄, 安村良男
Journal: Circulation (2008)
Developmental PBDE Exposure and IQ/ADHD in Childhood: A Systematic Review and Meta-analysis
Authors: Juleen Lam, Bruce P. Lanphear, David Bellinger, Daniel A. Axelrad, Jennifer McPartland, Patrice Sutton, Lisette Davidson, Natalyn Daniels, Saunak Sen, Tracey J. Woodruff
Journal: Environmental Health Perspectives (2017)

Background: In the United States, one in six children are affected by neurodevelopmental disorders, and polybrominated diphenyl ethers (PBDEs) in flame-retardant chemicals are measured ubiquitously in children. Objective: We conducted a systematic a systematic review regarding developmental exposure to PBDEs and intelligence or Attention Deficit/Hyperactivity Disorder (ADHD) and attention-related behavioral conditions in humans. Methods: We searched articles published up to 26 September 2016, and included original studies that quantified exposures to PBDEs incurred any time in proximity to conception or during in utero, perinatal, or childhood time periods. We evaluated the risk of bias of individual studies and the overall quality and strength of the evidence according to the Navigation Guide systematic review methodology. We established criteria in advance to identify studies that could be combined using random effects meta-analyses (DerSimonian-Laird method). Results: Fifteen studies met the inclusion criteria; 10 studies met the criteria for intelligence and nine for attention-related problems. We rated studies generally with "low" to "probably low" risk of bias and rated the overall body of evidence as "moderate" quality with "sufficient" evidence for an association between Intelligence Quotient (IQ) and PBDEs. Our meta-analysis of four studies estimated a 10-fold increase (in other words, times 10) in PBDE exposure associated with a decrement of 3.70 IQ points (95% confidence interval: 0.83, 6.56). We concluded the body of evidence was of "moderate" quality for ADHD with "limited" evidence for an association with PBDEs, based on the heterogeneity of association estimates reported by a small number of studies and the fact that chance, bias, and confounding could not be ruled out with reasonable confidence. Conclusion: We concluded there was sufficient evidence supporting an association between developmental PBDE exposure and reduced IQ. Preventing developmental exposure to PBDEs could help prevent loss of human intelligence. https://doi.org/10.1289/EHP1632
Stress Perfusion Coronary Flow Reserve Versus Cardiac Magnetic Resonance for Known or Suspected CAD
Authors: Shingo Kato, Naka Saito, Tatsuya Nakachi, Kazuki Fukui, Tae Iwasawa, Masataka Taguri, Masami Kosuge, Kazuo Kimura
Journal: Journal of the American College of Cardiology (2017)
Systemic Pharmacologic Therapies for Low Back Pain: A Systematic Review for an American College of Physicians Clinical Practice Guideline
Authors: Roger Chou, Richard Deyo, Janna Friedly, Andrea Skelly, Melissa Weimer, Rochelle Fu, Tracy Dana, Paul Kraegel, Jessica Griffin, Sara Grusing
Journal: Annals of Internal Medicine (2017)

Background: A 2007 American College of Physicians guideline addressed pharmacologic options for low back pain. New evidence and medications have now become available. Purpose: To review the current evidence on systemic pharmacologic therapies for acute or chronic nonradicular or radicular low back pain. Data Sources: Ovid MEDLINE (January 2008 through November 2016), Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, and reference lists. Study Selection: Randomized trials that reported pain, function, or harms of systemic medications versus placebo or another intervention. Data Extraction: One investigator abstracted data, and a second verified accuracy; 2 investigators independently assessed study quality. Data Synthesis: The number of trials ranged from 9 (benzodiazepines) to 70 (nonsteroidal anti-inflammatory drugs). New evidence found that acetaminophen was ineffective for acute low back pain, nonsteroidal anti-inflammatory drugs had smaller benefits for chronic low back pain than previously observed, duloxetine was effective for chronic low back pain, and benzodiazepines were ineffective for radiculopathy. For opioids, evidence remains limited to short-term trials showing modest effects for chronic low back pain; trials were not designed to assess serious harms. Skeletal muscle relaxants are effective for short-term pain relief in acute low back pain but caused sedation. Systemic corticosteroids do not seem to be effective. For effective interventions, pain relief was small to moderate and generally short-term; improvements in function were generally smaller. Evidence is insufficient to determine the effects of antiseizure medications. Limitations: Qualitatively synthesized new trials with prior meta-analyses. Only English-language studies were included, many of which had methodological shortcomings. Medications injected for local effects were not addressed. Conclusion: Several systemic medications for low back pain are associated with small to moderate, primarily short-term effects on pain. New evidence suggests that acetaminophen is ineffective for acute low back pain, and duloxetine is associated with modest effects for chronic low back pain. Primary Funding Source: Agency for Healthcare Research and Quality. (PROSPERO: CRD42014014735)
Identification of Multiple, Patient-Specific MLL Fusion Transcript Isoforms in Childhood Leukemia Using Anchored Multiplex PCR-Based Enrichment (AMP-E)
Authors: Sadia Afrin, Claus Meyer, Caedyn L. Stinson, Thy Pham, Timothy J.C. Bruxner, Nicola C. Venn, Toby N. Trahair, Rosemary Sutton, Rolf Marschalek, Lynn Fink, Andrew S. Moore
Journal: Blood (2016)
Factors Influencing the Induction of Pulmonary Tumors in Strain A Mice by Carcinogenic Hydrocarbons
Authors: Michael B. Shimkin, Egon Lorenz
Journal: JNCI Journal of the National Cancer Institute (1942)

Journal Article Factors Influencing the Induction of Pulmonary Tumors in Strain A Mice by Carcinogenic Hydrocarbons Get access Michael B. Shimkin, Michael B. Shimkin assistant surgeon National Cancer Institute, National Institute of Health, United States Public Health Service Search for other works by this author on: Oxford Academic PubMed Google Scholar Egon Lorenz Egon Lorenz senior biophysicist National Cancer Institute, National Institute of Health, United States Public Health Service Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 2, Issue 5, April 1942, Pages 499–510, https://doi.org/10.1093/jnci/2.5.499 Published: 01 April 1942
Macroscopic T-Wave Alternans
Authors: Peter K. Moore, Katie E. Raffel, Isaac R. Whitman
Journal: JAMA Internal Medicine (2017)

A man in his 40s with a history of alcohol abuse and hepatitis C cirrhosis presented to the emergency department with several weeks of subjective fever, abdominal cramping, and diarrhea. He had no history of cardiovascular disease and was not taking medications or supplements.