tbuckley/cabot-search huggingface dataset:
Abstract 90: The Spleen Is an Essential Regulator of Systemic Neovascularization In Vivo
Authors: Mohamed Ameen Ismahil, Tariq Hamid, Justin R Kingery, Sumanth D Prabhu
Journal: Circulation Research (2012)

Myocardial neovascularization play key role in the structural remodeling that occurs in heart failure. Prior studies have suggested an important role for splenic monocytes in tissue healing after myocardial infarction; however, the role of macrophages in regulating angiogenesis at the site of tissue injury is complex and not well defined. Moreover, it is unclear how the spleen regulates macrophage-mediated neovascularization. We hypothesized that the spleen plays a critical role in determining macrophage polarity that in turn influences tissue neovascularization. In 7 naïve and 7 splenectomized C57BL/6 mice (splenectomy performed 28 d prior), we performed in vivo Matrigel plug assays. Matrigel (0.6 mL/plug) was injected subcutaneously, either alone or together with peritoneal macrophages (∼20,000 cells/plug) isolated from syngeneic mice, and Matrigel plug neovascularization was assessed after 10 d. Masson trichrome staining and CD31 immunostaining was used to index vascularization. Circulating activated monocytes (CD11b+F480+) and endothelial progenitor cells (EPCs, CD34+Flk-1+) were assessed by flow cytometry. Flow cytometry of peripheral blood revealed significant reductions in circulating activated monocytes (2.20 ± 0.32 vs. 3.95 ± 0.49 %; p=0.002) and EPCs (0.0076 ± 0.0013 vs. 0.018 ± 0.0023; p=0.0002) in splenectomized mice as compared with naïve mice. Splenectomized mice also exhibited markedly reduced neovessel formation in Matrigel plugs indexed by morphometric and immunohistological analyses. The addition of peritoneal macrophages to the Matrigel plug restored vessel formation in splenectomized mice, but did not change neovascularization in naïve mice. Moreover, this restoration of neovascularization occurred with the addition of peritoneal macrophages polarized to an M2 phenotype (using interleukin [IL]-4 or IL-10) but not to an M1 phenotype. We conclude that the spleen is a key determinant of macrophage polarization and function that subsequently impacts the efficiency of tissue neovascularization. Hence, the spleen provides an anti-inflammatory and pro-angiogenic regulatory function in vivo that may be of central importance to the adequacy of myocardial repair and remodeling following tissue injury.
Radiation and Chemotherapy Bystander Effects in Colorectal Cancer
Authors: S Gorman, M Tosetto, O Howe, F Lyng, H Mulcahy, D O'Donoghue, J Hyland, D Fennelly, J Armstrong, K Sheahan, J O'Sullivan
Journal: Endoscopy (2006)

Background: Bystander effects occur when irradiated cells communicate with non-irradiated cells possibly via secreted factors, the non-irradiated cells then displaying characteristics of irradiated cells. Little is known regarding bystander radiation and chemotherapy effects alone and in combination in colorectal cancer.
KINETIC CHAIN RELATED RISK FACTORS FOR THE DEVELOPMENT OF LOWER EXTREMITY INJURIES IN WOMEN: A PROSPECTIVE STUDY
Authors: Ruth Verrelst, Damien Van Tiggelen, Roel De Ridder, Erik Witvrouw
Journal: British Journal of Sports Medicine (2017)

Background Attention has turned to the role of the overall kinetic chain function within the development of lower extremity (LE) injury, however few longitudinal studies have been conducted so far. Objective To prospectively determine kinetic chain related risk factors for the development of lower extremity (LE) injury in women. Design Prospective cohort design. Setting Department of Physical Education at the University of Ghent, Belgium. Participants Ninety healthy, female physical education students aged 19.33±0.87 years were tested. Assessment of Risk Factors Kinematic and VGRF parameters were measured during a triple hop jump (THJ). Cox regression analysis was used to identify the kinetic chain related risk factors for the development of LE injury. Main Outcome Measurements The LE injury follow-up was assessed using a weekly online questionnaire and a three-monthly retrospective control questionnaire. LE injury was diagnosed by an experienced medical doctor. Results Thirty-five participants were diagnosed with a LE injury. The results of this study identified that in the sagittal plane, decreased dynamic range of motion (ROM) of the pelvis segment ( P =0.043) and increased dynamic ROM of the knee and ankle joint ( P =0.041 and P =0.028 respectively) during landing phase are predictive parameters for the development of LE injury in females. No VGRF parameters were found to be of predictive value. Conclusions The pattern of decreased ROM of the pelvis segment in the sagittal plane combined with increased ROM of knee and ankle joint in the sagittal plane during landing phase, can be seen as decreased ability of equally absorbing forces throughout the kinetic chain during the landing phase of the THJ, which might lead to LE injury.
Baby-Led Weaning—Safe and Effective but Not Preventive of Obesity
Authors: Rajalakshmi Lakshman, Emma A. Clifton, Ken K. Ong
Journal: JAMA Pediatrics (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Association Between Medication Use and Performance on Higher Education Entrance Tests in Individuals With Attention-Deficit/Hyperactivity Disorder
Authors: Yi Lu, Arvid Sjölander, Martin Cederlöf, Brian M. D’Onofrio, Catarina Almqvist, Henrik Larsson, Paul Lichtenstein
Journal: JAMA Psychiatry (2017)

<h3>Importance</h3> Individuals with attention-deficit/hyperactivity disorder (ADHD) are at greater risk for academic problems. Pharmacologic treatment is effective in reducing the core symptoms of ADHD, but it is unclear whether it helps to improve academic outcomes. <h3>Objective</h3> To investigate the association between the use of ADHD medication and performance on higher education entrance tests in individuals with ADHD. <h3>Design, Setting, and Participants</h3> This cohort study observed 61 640 individuals with a diagnosis of ADHD from January 1, 2006, to December 31, 2013. Records of their pharmacologic treatment were extracted from Swedish national registers along with data from the Swedish Scholastic Aptitude Test. Using a within-patient design, test scores when patients were taking medication for ADHD were compared with scores when they were not taking such medication. Data analysis was performed from November 24, 2015, to November 4, 2016. <h3>Exposures</h3> Periods with and without ADHD medication use. <h3>Main Outcomes and Measures</h3> Scores from the higher education entrance examination (score range, 1-200 points). <h3>Results</h3> Among 930 individuals (493 males and 437 females; mean [SD] age, 22.2 [3.2] years) who had taken multiple entrance tests (n = 2524) and used ADHD medications intermittently, the test scores were a mean of 4.80 points higher (95% CI, 2.26-7.34;<i>P</i> &lt; .001) during periods they were taking medication vs nonmedicated periods, after adjusting for age and practice effects. Similar associations between ADHD medication use and test scores were detected in sensitivity analyses. <h3>Conclusions and Relevance</h3> Individuals with ADHD had higher scores on the higher education entrance tests during periods they were taking ADHD medication vs nonmedicated periods. These findings suggest that ADHD medications may help ameliorate educationally relevant outcomes in individuals with ADHD.
Downregulation of Human Endogenous Retrovirus Type K (HERV-K) Viral <i>env</i> RNA in Pancreatic Cancer Cells Decreases Cell Proliferation and Tumor Growth
Authors: Ming Li, Laszlo Radvanyi, Bingnan Yin, Kiera Rycaj, Jia Li, Raghavender Chivukula, Kevin Lin, Yue Lu, JianJun Shen, David Z. Chang, Donghui Li, Gary L. Johanning, Feng Wang-Johanning
Journal: Clinical Cancer Research (2017)

Abstract Purpose: We investigated the role of the human endogenous retrovirus type K (HERV-K) envelope (env) gene in pancreatic cancer. Experimental Design: shRNA was employed to knockdown (KD) the expression of HERV-K in pancreatic cancer cells. Results: HERV-K env expression was detected in seven pancreatic cancer cell lines and in 80% of pancreatic cancer patient biopsies, but not in two normal pancreatic cell lines or uninvolved normal tissues. A new HERV-K splice variant was discovered in several pancreatic cancer cell lines. Reverse transcriptase activity and virus-like particles were observed in culture media supernatant obtained from Panc-1 and Panc-2 cells. HERV-K viral RNA levels and anti-HERV-K antibody titers were significantly higher in pancreatic cancer patient sera (N = 106) than in normal donor sera (N = 40). Importantly, the in vitro and in vivo growth rates of three pancreatic cancer cell lines were significantly reduced after HERV-K KD by shRNA targeting HERV-K env, and there was reduced metastasis to lung after treatment. RNA-Seq results revealed changes in gene expression after HERV-K env KD, including RAS and TP53. Furthermore, downregulation of HERV-K Env protein expression by shRNA also resulted in decreased expression of RAS, p-ERK, p-RSK, and p-AKT in several pancreatic cancer cells or tumors. Conclusions: These results demonstrate that HERV-K influences signal transduction via the RAS–ERK–RSK pathway in pancreatic cancer. Our data highlight the potentially important role of HERV-K in tumorigenesis and progression of pancreatic cancer, and indicate that HERV-K viral proteins may be attractive biomarkers and/or tumor-associated antigens, as well as potentially useful targets for detection, diagnosis, and immunotherapy of pancreatic cancer. Clin Cancer Res; 23(19); 5892–911. ©2017 AACR.
Clinical Decision Support Systems and Antibiotic Prescribing—Reply
Authors: Matthew H. Samore, Gregory J. Stoddard, Stephen C. Alder, Bassam Haddadin, Michael A. Rubin, Kim Bateman
Journal: JAMA (2006)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Anticoagulation Prophylaxis in Asparaginase-Based Therapy in Adults with Acute Lymphoblastic Leukemia
Authors: Hassan Sibai, Umberto Falcone, Arjun Datt Law, Naoko Sakurai, Lalit Saini, Tian Q Wang, Andrew Stessman, Joseph Samuel, Eshetu G. Atenafu, Ali Hosni, Andre C. Schuh, Karen W.L. Yee, Vikas Gupta, Mark D. Minden, Steven M. Chan, Aaron D. Schimmer, Joseph M. Brandwein, Jack T Seki
Journal: Blood (2016)
Sidedness and TP53 mutations impact OS in anti-EGFR but not anti-VEGF treated mCRC - An analysis of the KRAS registry of the AGMT (Arbeitsgemeinschaft Medikamentöse Tumortherapie
Authors: Florian Huemer, Josef Thaler, Gudrun Piringer, Hubert Hackl, Lisa Pleyer, Clemens Hufnagl, Lukas Weiss, Richard Greil
Journal: Annals of Oncology (2017)
Use of QuantiFERON-TB Gold In-tube assay in screening for neutralizing anti-interferon-γ autoantibodies in patients with disseminated nontuberculous mycobacterial infection
Authors: U.-I. Wu, Y.-C. Chuang, W.-H. Sheng, H.-Y. Sun, Y.-T. Jhong, J.-Y. Wang, S.-C. Chang, J.-T. Wang, Y.-C. Chen
Journal: Clinical Microbiology and Infection (2017)
Severe Pneumococcal Pneumonia Causes Acute Cardiac Toxicity and Subsequent Cardiac Remodeling
Authors: Luis F. Reyes, Marcos I. Restrepo, Cecilia A. Hinojosa, Nilam J. Soni, Antonio Anzueto, Bettina L. Babu, Norberto Gonzalez-Juarbe, Alejandro H. Rodriguez, Alejandro Jimenez, James D. Chalmers, Stefano Aliberti, Oriol Sibila, Vicki T. Winter, Jacqueline J. Coalson, Luis D. Giavedoni, Charles S. Dela Cruz, Grant W. Waterer, Martin Witzenrath, Norbert Suttorp, Peter H. Dube, Carlos J. Orihuela
Journal: American Journal of Respiratory and Critical Care Medicine (2017)

Rationale: Up to one-third of patients hospitalized with pneumococcal pneumonia experience major adverse cardiac events (MACE) during or after pneumonia. In mice, Streptococcus pneumoniae can invade the myocardium, induce cardiomyocyte death, and disrupt cardiac function following bacteremia, but it is unknown whether the same occurs in humans with severe pneumonia.Objectives: We sought to determine whether S. pneumoniae can (1) translocate the heart, (2) induce cardiomyocyte death, (3) cause MACE, and (4) induce cardiac scar formation after antibiotic treatment during severe pneumonia using a nonhuman primate (NHP) model.Methods: We examined cardiac tissue from six adult NHPs with severe pneumococcal pneumonia and three uninfected control animals. Three animals were rescued with antibiotics (convalescent animals). Electrocardiographic, echocardiographic, and serum biomarkers of cardiac damage were measured (troponin T, N-terminal pro-brain natriuretic peptide, and heart-type fatty acid binding protein). Histological examination included hematoxylin and eosin staining, immunofluorescence, immunohistochemistry, picrosirius red staining, and transmission electron microscopy. Immunoblots were used to assess the underlying mechanisms.Measurements and Main Results: Nonspecific ischemic alterations were detected by electrocardiography and echocardiography. Serum levels of troponin T and heart-type fatty acid binding protein were increased (P < 0.05) after pneumococcal infection in both acutely ill and convalescent NHPs. S. pneumoniae was detected in the myocardium of all NHPs with acute severe pneumonia. Necroptosis and apoptosis were detected in the myocardium of both acutely ill and convalescent NHPs. Evidence of cardiac scar formation was observed only in convalescent animals by transmission electron microscopy and picrosirius red staining.Conclusions: S. pneumoniae invades the myocardium and induces cardiac injury with necroptosis and apoptosis, followed by cardiac scarring after antibiotic therapy, in an NHP model of severe pneumonia.
Data normalization considerations for digital tumor dissection
Authors: Aaron M. Newman, Andrew J. Gentles, Chih Long Liu, Maximilian Diehn, Ash A. Alizadeh
Journal: Genome biology (2017)

In a recently published article in Genome Biology, Li and colleagues introduced TIMER, a gene expression deconvolution approach for studying tumor-infiltrating leukocytes (TILs) in 23 cancer types profiled by The Cancer Genome Atlas. Methods to characterize TIL biology are increasingly important, and the authors offer several arguments in favor of their strategy. Several of these claims warrant further discussion and highlight the critical importance of data normalization in gene expression deconvolution applications. Please see related Li et al correspondence: www.dx.doi.org/10.1186/s13059-017-1256-5 and Zheng correspondence: www.dx.doi.org/10.1186/s13059-017-1258-3
Multi-omics of 34 colorectal cancer cell lines - a resource for biomedical studies
Authors: Kaja C. G. Berg, Peter W. Eide, Ina A. Eilertsen, Bjarne Johannessen, Jarle Bruun, Stine A. Danielsen, Merete Bjørnslett, Leonardo A. Meza-Zepeda, Mette Eknæs, Guro E. Lind, Ola Myklebost, Rolf I. Skotheim, Anita Sveen, Ragnhild A. Lothe
Journal: Molecular Cancer (2017)

Colorectal cancer (CRC) cell lines are widely used pre-clinical model systems. Comprehensive insights into their molecular characteristics may improve model selection for biomedical studies. We have performed DNA, RNA and protein profiling of 34 cell lines, including (i) targeted deep sequencing (n = 612 genes) to detect single nucleotide variants and insertions/deletions; (ii) high resolution DNA copy number profiling; (iii) gene expression profiling at exon resolution; (iv) small RNA expression profiling by deep sequencing; and (v) protein expression analysis (n = 297 proteins) by reverse phase protein microarrays. The cell lines were stratified according to the key molecular subtypes of CRC and data were integrated at two or more levels by computational analyses. We confirm that the frequencies and patterns of DNA aberrations are associated with genomic instability phenotypes and that the cell lines recapitulate the genomic profiles of primary carcinomas. Intrinsic expression subgroups are distinct from genomic subtypes, but consistent at the gene-, microRNA- and protein-level and dominated by two distinct clusters; colon-like cell lines characterized by expression of gastro-intestinal differentiation markers and undifferentiated cell lines showing upregulation of epithelial-mesenchymal transition and TGFβ signatures. This sample split was concordant with the gene expression-based consensus molecular subtypes of primary tumors. Approximately ¼ of the genes had consistent regulation at the DNA copy number and gene expression level, while expression of gene-protein pairs in general was strongly correlated. Consistent high-level DNA copy number amplification and outlier gene- and protein- expression was found for several oncogenes in individual cell lines, including MYC and ERBB2. This study expands the view of CRC cell lines as accurate molecular models of primary carcinomas, and we present integrated multi-level molecular data of 34 widely used cell lines in easily accessible formats, providing a resource for preclinical studies in CRC.
Можливості і проблеми застосування гепатопротекторів у хворих на хронічний гепатит С при супутньому автоімунному синдромі
Authors: I.Ya. Hospodarskyi, K.V. Volynets, V.V. Hrushko
Journal: GASTROENTEROLOGY (2014)

Проведене вивчення клініко-біохімічних показників ефективності гепатопротекторів у 32 хворих на хронічний гепатит С без ознак цирозу при супутньому автоімунному компоненті. Виявлена суттєва різниця терапевтичного впливу біофлавоноїдних гепатопротекторів та гепатопротекторів на основі орнітину на показники функції печінки та імунної системи, а також на перебіг гепатиту С.
Therapy of Small Cell Lung Cancer (SCLC) with a Topoisomerase-I–inhibiting Antibody–Drug Conjugate (ADC) Targeting Trop-2, Sacituzumab Govitecan
Authors: Jhanelle E. Gray, Rebecca S. Heist, Alexander N. Starodub, D. Ross Camidge, Ebenezer A. Kio, Gregory A. Masters, W. Thomas Purcell, Michael J. Guarino, Jamal Misleh, Charles J. Schneider, Bryan J. Schneider, Allyson Ocean, Tirrell Johnson, Leena Gandhi, Kevin Kalinsky, Ronald Scheff, Wells A. Messersmith, Serengulam V. Govindan, Pius P. Maliakal, Boyd Mudenda, William A. Wegener, Robert M. Sharkey, David M. Goldenberg
Journal: Clinical Cancer Research (2017)

Purpose: We evaluated a Trop-2-targeting antibody conjugated with SN-38 in metastatic small cell lung cancer (mSCLC) patients.Experimental Design: Sacituzumab govitecan was studied in patients with pretreated (median, 2; range, 1-7) mSCLC who received either 8 or 10 mg/kg i.v. on days 1 and 8 of 21-day cycles. The primary endpoints were safety and objective response rate (ORR); duration of response, progression-free survival (PFS), and overall survival (OS) were secondary endpoints.Results: Sixty percent of patients showed tumor shrinkage from baseline CTs. On an intention-to-treat basis (N = 50), the ORR was 14% (17% for the 10-mg/kg group); the median response duration, 5.7 months; the clinical benefit rate (CBR ≥4 months), 34%; median PFS, 3.7 months; and median OS, 7.5 months. There was a suggested improvement in PR, CBR, and PFS with sacituzumab govitecan in second-line patients who were sensitive to first-line therapy, but no difference between first-line chemosensitive versus chemoresistant patients in the overall population. There was a statistically significant higher OS in those patients who received prior topotecan versus no topotecan therapy in a small subgroup. Grade ≥3 adverse events included neutropenia (34%), fatigue (13%), diarrhea (9%), and anemia (6%). Trop-2 tumor staining was not required for patient selection. No antibodies to the drug conjugate or its components were detected on serial blood collections.Conclusions: Sacituzumab govitecan appears to have a safe and effective therapeutic profile in heavily pretreated mSCLC patients, including those who are chemosensitive or chemoresistant to first-line chemotherapy. Additional studies as a monotherapy or combination therapy are warranted. Clin Cancer Res; 23(19); 5711-9. ©2017 AACR.
Tumor Hyaluronan May Predict Benefit From PEGPH20 When Added to nab Paclitaxel/Gemcitabine in Patients With Previously Untreated Metastatic Pancreatic Ductal Adenocarcinoma (mPDA)
Authors: Andrew Hendifar, Andrea Bullock, Tara Seery, Lei Zheng, Darren Sigal, Paul Ritch, Fadi Braiteh, Mark Zalupski, Nathan Bahary, William Harris, Jie Pu, Fangru Lian, Junming Zhu, Wilson Wu, Joaquina Baranda, Ping Jiang, Sunil Hingorani
Journal: Annals of Oncology (2017)
Outcome of Autologous Hematopoietic Stem Cell Transplantation in Relapsed and Refractory Multiple Myeloma
Authors: Lauren Westfall Veltri, Denái R. Milton, Nina Shah, Krina Patel, Yago Nieto, Partow Kebriaei, Uday R. Popat, Simrit Parmar, Betul Oran, Stefan O. Ciurea, Chitra Hosing, Hans Lee, Elisabet E. Manasanch, Robert Z. Orlowski, Elizabeth J. Shpall, Richard E. Champlin, Muzaffar H. Qazilbash, Qaiser Bashir
Journal: Blood (2016)
Reducing Bodies in the Adrenal Medulla Fixed With Zenker-Formol
Authors: S. S. Spicer, M.D., Thelma B. Dunn, M.D.
Journal: JNCI Journal of the National Cancer Institute (1960)

The adrenal medulla fixed in Helly's fluid (Zenker-formol) reveals hematoxyphilic particles. Histochemical studies of these strongly reducing particles indicate they are composed of catechol amine. Spontaneous mouse pheochromocytomas fixed in Helly's fluid show similar reducing bodies conspicuously.