tbuckley/cabot-search huggingface dataset:
Abstract 14956: Out-of-hospital Cardiac Arrest and Bystander CPR in Kent County, Michigan: A Geospatial Analysis
Authors: Amy Uber, Todd Chassee, Rick C Sadler, Joshua C Reynolds
Journal: Circulation (2015)

Introduction: Geographic clustering of OHCA &amp; bystander CPR (bCPR) has been demonstrated in some US cities. We assessed for geospatial distribution patterns within our county. Objectives: 1) Characterize bCPR performance; 2) Test for geographic clustering of OHCA &amp; bCPR; 3) Identify hot spots &amp; cold spots, defined as areas with disproportionately greater and lesser chance of bystander intervention within a concentration of OHCA. Hypothesis: OHCA &amp; bCPR is geographically clustered within our region, resulting in hot and cold spots of bystander intervention. Methods: Retrospective, observational, population-based, cohort study using the CARES dataset for Kent County, Michigan. We included adult, non-traumatic, EMS-treated OHCA from 2010-14, excluding cases with on-site medical care. We abstracted EMS dispatch addresses, bCPR, &amp; patient characteristics, then geocoded street addresses (ArcGIS) to create a population-adjusted density map of OHCA annual incidence. Moran’s I test assessed for spatial autocorrelation of population-weighted cardiac arrest rate by census block. Difference mapping identified relative differences between cases with/without bCPR, &amp; Getis-Ord Gi statistic assessed for spatial clustering of bCPR, identifying hot and cold spots. Results: Of 1,632 eligible subjects, 1,167 met inclusion criteria. bCPR occurred in 436 (37%), &amp; was performed by family members (58%), lay persons (34%), &amp; off-duty medical providers (8%). Specific techniques included compressions/ventilations (28%), compression only (35%), ventilation only (1%), &amp; unknown (36%). Geospatial analysis revealed significant clustering of OHCA ranging from 0 to &gt;60 annual cases per 100,000 population (p&lt;0.001). Relative difference in bCPR ranged from -11.2 to 0.9 across geographic locales, with a paucity of bCPR (-11.2 to -2.0) in urban and suburban areas. We identified central Grand Rapids &amp; southern suburbs as primary cold spots (99% confidence), whereas eastern &amp; northeastern suburbs contained hot spots (99% confidence). Conclusion: The minority of OHCA received bCPR. OHCA &amp; bCPR are geographically clustered, &amp; bCPR is more/less likely to occur in certain areas. Distinguishing hot &amp; cold spots affords opportunity for targeted public health initiatives.
Abstract 18313: Predictors of Death or Extracorporeal Membrane Oxygenation Support After Surgery for Adult Congenital Heart Disease
Authors: Stephen J Dolgner, Titus Chan, Eric V Krieger, Jacob Wilkes, Susan L Bratton, Ravi R Thiagarajan, Cindy S Barrett
Journal: Circulation (2016)

Introduction: Adults with congenital heart disease (ACHD) who undergo cardiac surgery are at risk for poor outcomes, including death and extracorporeal membrane oxygenation support (ECMO). Prior st...
Abstract 351: Epigenetic mechanisms regulate DNA amplification and drug-resistant gene selection
Authors: Sweta Mishra, Capucine Van Rechem, Damayanti Chakraborty, Johnathan R. Whetstine
Journal: Cancer Research (2018)

Abstract DNA copy gains and amplifications are often associated with tumorigenesis, poor prognosis and drug resistance. Little is known about how these regions of the genome are selected for amplification. Our group discovered that modulation of epigenetic factors and their associated chromatin states control DNA copy gains of drug-resistant regions. In fact, transient site-specific copy number gains (TSSGs) occur in both normal and cancer cells upon manipulation of a histone H3 lysine 9/36 (H3K9/36) tri-demethylase KDM4A. However, the mechanism by which KDM4A is targeted to specific genomic regions and whether additional chromatin regulators are involved in this process were unknown. To begin addressing these questions, we performed a series of unbiased genetic screens against all the histone lysine-modifying enzymes and followed their ability to generate copy gains of regions within chromosome 1. Our screen uncovered insights into both KDM4A targeting and the identification of other epigenetic factors modulating DNA amplification of drug-resistant regions of the genome. We have uncovered KDM4A-independent DNA amplifications, which established that additional regions are undergoing epigenetic control for amplification. These currently unpublished data will be discussed at the meeting. Citation Format: Sweta Mishra, Capucine Van Rechem, Damayanti Chakraborty, Johnathan R. Whetstine. Epigenetic mechanisms regulate DNA amplification and drug-resistant gene selection [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 351.
Abstract 248: Comparison Of Transplantation Of Bone Marrow-derived Stem Cells, Adipose-derived Stem Cells And Endometrium- Derived Stem Cells In The Infarcted Heart
Authors: Xin YangHu, KanWang, Jian-anWang
Journal: Circulation Research (2014)

Background: A variety of adult stem cells have been transplanted into the infarcted heart to cure myocardial infarction(MI), however, comparative studies are lacking to show more suitable source of cells for transplantation. Mesenchymal stem cells hold promise for myocardial regeneration therapy. Derivation of these cells from the endometrium tissue might be easier compared to bone marrow and adipose tissue. However,the in vivo fate of endometrium stem cells (EnSCs) in the infarcted heart has never been compared directly to mesenchymal cells derived from bone marrow(BMMSCs) and adipose tissue(AdMSCs). Methods: EnSCs, AdMSCs and BMMSCs were isolated from healthy donors were characterized using flow cytometry for surface markers identification and microscopy for cell morphology. They were characterized with β-actin promoter driving firefly luciferase and green fluorescent protein (Fluc-GFP) double fusion reporter gene, and were characterized using flow cytometry, bioluminescence imaging (BLI) and luminometr...
Abstract LB-207: A novel role for polycomb group protein BMI1 in prostate cancer
Authors: Sen Zhu, Dongyu Zhang, Lin Yan, Weihua Jiang, Qipeng Liu, Rui Wang, Bo Xia, Jonathan C. Zhao, Rong-Fu Wang, Xiaobing Shi, Hung-ming Lam, Xuesen Dong, Jindan Yu, Kaifu Chen, Qi Cao
Journal: Cancer Research (2018)

Abstract BMI1, a Polycomb Group (PcG) protein, plays a critical role in epigenetic regulation of cancer stem cell self-renewal, cell differentiation and proliferation. BMI1 is upregulated in multiple types of cancer, including prostate cancer. As a key component of Polycomb Repressive Complex 1 (PRC1), BMI1 can exert its oncogenic functions by enhancing the enzymatic activities of RING1B to ubiquitinate histone H2A at lysine 119 and repress gene transcription. Here we report a PRC1-independent role of BMI1 that is critical for castrate-resistant prostate cancer (CRPC) progression. BMI1 binds the androgen receptor (AR) and prevents MDM2-mediated AR protein degradation, resulting in sustained AR signaling in prostate cancer cells. More importantly, we demonstrate that targeting BMI1 effectively inhibits tumor growth of xenografts that have developed resistance to surgical castration and enzalutamide treatment. These results suggest that blocking BMI1 alone or in combination with anti-AR therapy can be more efficient to suppress prostate tumor growth. Citation Format: Sen Zhu, Dongyu Zhang, Lin Yan, Weihua Jiang, Qipeng Liu, Rui Wang, Bo Xia, Jonathan C. Zhao, Rong-Fu Wang, Xiaobing Shi, Hung-ming Lam, Xuesen Dong, Jindan Yu, Kaifu Chen, Qi Cao. A novel role for polycomb group protein BMI1 in prostate cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr LB-207.
Abstract 20501: Left Ventricular Flow Dynamics in the Presence of Severe Mitral Annular Calcification - An In Vitro Study
Authors: Batoul El Sayegh, Giuseppe DiLabbio, Gregg S Pressman, Edinrin Obasare, Lyes Kadem
Journal: Circulation (2016)

Introduction: Normal flow patterns within the left ventricle (LV) optimize energy efficiency. Cardiac disease leads to disturbed flow and suboptimal LV performance. Mitral annular calcification (MA...
Relationships Between Alterations in Amine Metabolism and Blood Pressure
Authors: Albertsjoerdsma
Journal: Circulation Research (1961)

The principal processes of synthesis and metabolism for aromatic amines have been discussed in terms of their status in essential hypertension and in relation to alterations induced by enzyme inhibitors with hypotensive properties. From a practical standpoint, the clinical use of monoamine oxidase inhibitors and a decarboxylase inhibitor (α-methyldopa) approach the achievement of medical sympathectomy without prohibitive side effects. From a basic standpoint, use of these inhibitors as biochemical tools affords a new perspective into connections between aromatic amine metabolism and blood-pressure regulation. A combined pharmacological-biochemical approach in man will be an important phase of future investigations.
Abstract 485: Modulation of microRNA expression in EGFR-tyrosine kinase inhibitor-resistant non-small cell lung cancer cell lines
Authors: Inamul Haque, Hannah Motes, Mukut Sharma, Emma Borrego-Diaz Reyes, Andrew K. Godwin, Chao H. Huang
Journal: Cancer Research (2018)

Abstract Objectives: Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are now standard of care in lung cancer patients with EGFR mutation. Invariably, these patients develop resistance and require treatment with another EGFR-TKI or chemotherapy. The gatekeeper mutation T790M is responsible for about 50% of resistance. Other mechanisms may also confer resistance in these patients. High expression of microRNA-10b (miRNA-10b) is associated with worse prognosis in resected lung cancer patients with EGFR mutation. Both miRNA-27a and -27b are associated with increased expression of c-met, which is a potential mechanism of resistance to EGFR-TKI. The goal of this study is to determine the optimal treatment and miRNA characteristic of EGFR mutation lung cancer cells resistant to EGFR-TKI. We hypothesized that miRNA-10b, -27a and -27b modulated Erlotinib (ERL) resistance by different EGFR-TKIs and chemotherapeutic agents in EGFR mutant NSCLC cell lines. Materials and Methods: Lung cancer cell lines with EGFR mutation HCC827 and HCC4006 resistant to ERL were generated. ERL-resistant cells were then treated with 10 nM afatinib (Afa), 0.5 mg/mL pemetrexed (Pem), and combination of 10 nM Afa/0.5mg/mL Pem for 72h. The cells were harvested and microRNA assay was performed by real-time PCR. NCI-H460 cell line without EGFR mutation was used as control. Results: We observed increased expression of miRNA-10b, miRNA-27a and miRNA-27b in ERL-resistant HCC4006 compared to control cells NCI-H460. There was an upregulation of miRNA-27a and -27b but not of miRNA-10b in ERL-resistant HCC827. Treatment of ERL-resistant cells with Afa, Pem, and combined treatment changed the expression of miRNA-10b, miRNA-27a and miRNA-27b. These miRNAs were downregulated by 2nd-generation EGFR-TKI therapy alone or in combination with Pem. Different treatments had different effects on these miRNAs. There might be differences in miRNA regulation in different cell lines when treated with EGFR-TKI or chemotherapy. Our results suggest that microRNA may be used as targets for developing novel approach to treating patients with EGFR-TKI resistance. Conclusion: This study may have implications in the management of patients resistant to EGFR TKI. MicroRNA analysis showed upregulation of several microRNAs that should be confirmed in clinical setting. Citation Format: Inamul Haque, Hannah Motes, Mukut Sharma, Emma Borrego-Diaz Reyes, Andrew K. Godwin, Chao H. Huang. Modulation of microRNA expression in EGFR-tyrosine kinase inhibitor-resistant non-small cell lung cancer cell lines [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 485.
Pulsatile Cardiac Tissue Grafts Using a Novel Three-Dimensional Cell Sheet Manipulation Technique Functionally Integrates With the Host Heart, In Vivo
Authors: AkiraFuruta, ShunichiroMiyoshi, YujiItabashi, TatsuyaShimizu, ShinichiroKira, KeikoHayakawa, NobuhiroNishiyama, KojiroTanimoto, YokoHagiwara, ToshiakiSatoh, KeiichiFukuda, TeruoOkano, SatoshiOgawa
Journal: Circulation Research (2006)

We devised a method of fabricating easily transplantable scaffoldless 3D heart tissue, made with a novel cell-sheet (CS) technology from cultured cardiomyocytes using a fibrin polymer coated dish. In the present study, we tested in vivo electrical communication which is essential for improving heart function between the host heart and the grafted CS. The epicardial surface of the ventricle of an anesthetized open-chest nude rat was ablated by applying a heated metal. Bilayered CS was obtained from neonatal rat primary culture. CS was transplanted onto the injured myocardial surface (sMI) (sMI+sheet group). The rats were allowed to recover for 1 to 4 weeks, to stabilize the grafts. Action potentials (APs) from the excised perfused heart were monitored by the fluorescence signal of di-4ANEPPS with a high speed charge-coupled device camera. The APs were observed under epicardial pacing of the host heart or the CS grafts. The pacing threshold of the current output was measured in the sMI+sheet group and in th...
Abstract 14920: Elevation in Troponin-I is Associated With the Presence of Coronary Artery Disease and Increased Mortality in New Onset Atrial Fibrillation
Authors: Dustin Hillerson, Amrita Karve, William F Armstrong
Journal: Circulation (2016)

Introduction: Patients with new-onset atrial fibrillation (AF) present with a range of symptoms including chest discomfort or dyspnea. Troponin-I (Tn-I) is occasionally elevated in the absence of o...
Abstract 542: A disintergrin and metalloprotease (ADAM) 15 promotes non-small cell lung cancer (NSCLC) proliferation through cytosolic domain, not extra-cytosolic metalloprotease activity
Authors: Hsin-Han Hou
Journal: Cancer Research (2018)

Abstract Emerging evidence indicates that proteins of a disintergrin and metalloprotease (ADAM) family contribute to cancer progression and metastasis. ADAM15 is up-regulated in multiple cancers and the enzymatic activities of its extracellular metalloprotrease domain promote cancer proliferation and migration by mediating the ErbB signaling pathway. In lung cancer, the enzymatic activity of ADAM15 extracellular domain is reported to be independent of ErbB signaling, indicating an alternative mechanism in promoting lung cancer progression. However, the role of ADAM15 in non-small cell lung cancer (NSCLC) and its correlation to patient prognosis is not well understood. This study demonstrated the longest ADAM15 isoforms, isoform 6 (i6), which contained the most cytoplasmic Src homology 3 (SH3) binding motifs, was significantly up-regulated in progressive NSCLC cell. Comparison of i6 and isoform 1 (i1) revealed that the cytoplasmic domain was essential for NSCLC cell proliferation and aggressiveness. ADAM15 i6, but not i1, mediated the activation the activation of the Src homolog 2 domain containing (Shc) through physical interaction with growth factor receptor-bound protein 2 (Grb2). ADAM15 i6 overexpression promoted NSCLC cell growth in a xenograft mouse model, while ADAM15 or Grb2 knockdown resulted in tumor shrinkage. ADAM15 was overexpressed in primary NSCLC tissues compared to adjacent normal tissues. Patients with ADAM15 i6 high-expressing lung tumors had shorter survival times. Thus, a novel mechanism of the ADAM15 cytoplasmic domain is identified in NSCLC tumor progression. This may help shed light on the molecular mechanisms of ADAM proteins and facilitate development of novel therapy for NSCLC. Citation Format: Hsin-Han Hou. A disintergrin and metalloprotease (ADAM) 15 promotes non-small cell lung cancer (NSCLC) proliferation through cytosolic domain, not extra-cytosolic metalloprotease activity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 542.
Electrophysiological Characteristics and Catheter Ablation in Patients With Paroxysmal Right Atrial Fibrillation
Authors: Yenn-JiangLin, Ching-TaiTai, TsairKao, Han-WenTso, Jin-LongHuang, SatoshiHiga, YogaYuniadi, Bien-HsienHuang, Tu-YingLiu, Pi-ChangLee, Ming-HsiungHsieh, Shih-AnnChen
Journal: Circulation (2005)

Background— Catheter ablation of the right atrial (RA) substrate has had variable efficacy in curing paroxysmal atrial fibrillation (PAF), suggesting that RA substrate ablation can play an important role in the treatment of atrial fibrillation (AF) in some patients. The aim of this study was to investigate the electrophysiological characteristics and ablation strategy and its results in a specific group of patients with paroxysmal RA-AF. Methods and Results— The study population consisted of 13 patients (8 men; age, 64±15 years) with drug-refractory (2±1 drugs), frequent episodes of PAF. Provocation maneuvers did not reveal any ectopic beat–initiating AF. However, rapid atrial pacing easily induced AF. Activation mapping during sinus rhythm, atrial pacing, and AF was visualized by using a noncontact mapping system. Noncontact mapping revealed RA reentry (6 patients with single-loop circuits and 7 with double-loop circuits) with conduction through channels between lines of block, crista terminalis gaps, an...
Conditioning by Cortisol for the Production of Acute Massive Myocardial Necroses During Neuromuscular Exertion
Authors: Hansselye
Journal: Circulation Research (1958)

Following pretreatment with cortisol and NaH2PO4, a brief period of neuromuscular stress regularly produces massive myocardial necroses in the rat. Pretreatment with desoxycorticosterone and the phosphate is less effective in this respect.
Abstract 11721: Feasibility of Using a Leadless Patch Heart Rhythm Monitor to Measure Atrial Fibrillation Burden in Community-Based Epidemiological Studies: The Multiethnic Study of Atherosclerosis (MESA)
Authors: Lin Y Chen, Nicholas S Roetker, Aaron R Folsom, Alvaro Alonso, Susan R Heckbert
Journal: Circulation (2015)

Background: Whether or not higher atrial fibrillation (AF) burden (% time a person is in AF) is associated with higher risk of adverse outcomes such as stroke remains controversial. Research on AF burden in community-based studies has been hampered by lead-based heart rhythm monitors that only record continuously for short durations and have poor patient compliance. Hypothesis: We hypothesized that it is feasible to use a novel leadless patch monitor (Zio ® Patch [iRhythm Technologies, Inc, San Francisco, CA] that can record continuously for 2 weeks) to investigate AF burden in a sample of MESA participants. Methods: Between November 2013 and January 2014, we included 45 (mean age, 71.6 ± 6.2 years; 58% men) participants at the Minnesota field center without prior diagnoses or symptoms suggestive of AF or other arrhythmias; 30 and 15 were randomized to self-application (Group 1) and application by MESA staff (Group 2), respectively. Monitors were mailed to Group 1 and were applied at the field centers by MESA staff for Group 2 participants. We compared the wear time and analyzable time between the 2 groups. Results: The mean and median wear and analyzable times were not significantly different between the 2 groups (Table). Only 1 participant in Group 1 wore the monitor for &lt;2 days. Two (4%) participants were diagnosed by the monitor with AF (1 with burden of 0.02% and 1 with burden of 1.34%) and 1 with frequent non-sustained ventricular tachycardia; all participants were referred to their physicians for appropriate treatment and none suffered serious consequences. Conclusions: A leadless continuous recording patch monitor can be used effectively to investigate AF burden in asymptomatic subjects in community-based studies. Self-application of the monitor is equivalent to application by research staff which further facilitates the use of this monitor in community-based studies.
Low Birth Weight, a Risk Factor for Cardiovascular Diseases in Later Life, Is Already Associated With Elevated Fetal Glycosylated Hemoglobin at Birth
Authors: ThiemoPfab, TorstenSlowinski, MichaelGodes, HorstHalle, FriedrichPriem, BertholdHocher
Journal: Circulation (2006)

Background— It remains unclear whether the association between low birth weight and insulin resistance in adulthood has its origin in utero or whether it develops later in life depending on predisposition and exogenous factors. Methods and Results— Total glycosylated hemoglobin (TGH) was quantified at delivery in 1295 mother/child pairs serving as a surrogate of maternal and fetal glycemia. Multivariable regression analysis considering gestational age at delivery, the child’s sex, maternal body mass index, and smoking during pregnancy revealed that an increase in TGH by 1% in the child was significantly associated with a mean birth weight reduction of 135 g (P<0.0001), whereas the same increase in the mother was associated with a mean birth weight increase of 88 g (P<0.0001). The ratio of fetal/maternal TGH suggests that lighter newborns have a higher percentage of TGH than would be expected from maternal TGH. Conclusions— The study demonstrates for the first time in a large population that there is an in...
Abstract 16951: Polycystic Kidney Disease Aortic Endothelial Cells Show an Abnormal Angiogenic Response to the Hedgehog Ligand
Authors: Federico Franchi, Karen M Peterson, Ezequiel J Tolosa, Peter C Harris, Martin E Fernandez-Zapico, Martin G Rodriguez-Porcel
Journal: Circulation (2016)

Introduction: Polycystic Kidney Disease (PKD), a common renal ciliopathy, is characterized by impaired vascular structure and function, prior to changes in blood pressure or renal function. The can...
Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial
Authors: C. Adler Sørensen, E. Fuglsang, C. Sværke Jørgensen, R. Pilmann Laursen, A. Larnkjær, C. Mølgaard, C. Ritz, K.F. Michaelsen, K. Angeliki Krogfelt, H. Frøkiær
Journal: Clinical Microbiology and Infection (2018)

To examine the effect of a combination of probiotics on the antibody response to pneumococcal and pertussis vaccination in healthy Danish children, aged 8-14 months, at the time of starting day care. Moreover, the cytokine response to lipopolysaccharide of whole blood was assessed.A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a 6-month intervention period, and blood samples were drawn at the start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin-induced interleukin-6 (IL-6) and interferon-γ (IFN-γ) production in blood were analysed by Luminex and ELISA.There was no significant difference between the average individual changes from baseline to end of study in antibody concentrations for S. pneumoniae for both the probiotics (340.4% ± 11.2%) and the placebo group (382.9% ± 10.4%) (p 0.525), nor for B. pertussis toxin in the two groups (probiotics 190.1% ± 12.6% versus placebo 238.8% ± 1.1%, p 0.340). The average individual change in IL-6 concentration was significantly lower in the probiotics versus the placebo group (2.9% ± 10.3% versus 33.7% ± 9.0%, p 0.024), whereas there was no difference in IFN-γ concentration (0.0% ± 0.2% versus -0.2% ± 0.1%, p 0.279).The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.