tbuckley/cabot-search huggingface dataset:
The impact of tumor-to-nipple distance and multicentricity on occult nipple-areola complex involvement and local recurrence in the setting of nipple-sparing mastectomy: A meta-analysis and systematic review.
Authors: Hai-Tong Lyu, Sheng-Li An, Guo-Chun Zhang, Chong-Yang Ren, Ling-Zhu Wen, Yu-Mei Shi, Ning Liao
Journal: Journal of Clinical Oncology (2016)

1041 Background: Nipple-sparing mastectomy (NSM) improved patients’ mental and aesthetics results and showed safe oncologic outcomes. However, there were still controversies about the indications for NSM, especially tumor-to-nipple distance (TND) and multicentricity and there was limited evidence about predictive factors of local recurrence (LR). Methods: A systematic review was conducted according to the PRISMA guidelines. Studies reported on the association of TND or multicentricity and occult NAC involvement were included in the meta-analysis. Studies reported on TND or multicentricity and LR in the setting of NSM were included in the systematic review. We computed the Relative Risks (RRs) with 95% confidence intervals (CIs) from the original studies. Results: 1544 articles were identified from electronic search. Ultimately, seventeen clinical studies published from 1985 to 2015 with 4099 patients met selection criteria. Seven studies reported TND and occult NAC involvement. The Relative Risk (RR) for the groups of “TND < 1cm versus TND > 1cm”, “TND < 2cm versus TND > 2cm”, “TND < 3cm versus TND > 3cm” and “TND < 4cm versus TND > 4cm” were 7.95, 3.79, 2.71 and 3.43 respectively and the outcomes were all statistically significant. Six studies reported multicentricity and occult NAC involvement and showed no statistically significant difference. Eight studies reported specific inclusion criteria for TND in patients underwent NSM were included in the systematic review. Studies of TND > 1cm and TND > 2cm had mean LR rate of 3.3% and 5.3% respectively. Conclusions: This meta-analysis and systematic review showed that TND = 1cm could be considered as a cutoff to select NSM candidates for decreasing risk of occult NAC involvement. Besides, selecting patients of TND > 1cm or TND > 2cm for NSM may not increase LR. We also found that multicentricity was not associated with occult NAC involvement.
Microfluidic Transduction Harnesses Mass Transport Principles to Enhance Gene Transfer Efficiency
Authors: Reginald Tran, David R. Myers, Gabriela Denning, Jordan E. Shields, Allison M. Lytle, Hommood Alrowais, Yongzhi Qiu, Yumiko Sakurai, William C. Li, Oliver Brand, Joseph M. Le Doux, H. Trent Spencer, Christopher B. Doering, Wilbur A. Lam
Journal: Molecular Therapy (2017)

Ex vivo gene therapy using lentiviral vectors (LVs) is a proven approach to treat and potentially cure many hematologic disorders and malignancies but remains stymied by cumbersome, cost-prohibitive, and scale-limited production processes that cannot meet the demands of current clinical protocols for widespread clinical utilization. However, limitations in LV manufacture coupled with inefficient transduction protocols requiring significant excess amounts of vector currently limit widespread implementation. Herein, we describe a microfluidic, mass transport-based approach that overcomes the diffusion limitations of current transduction platforms to enhance LV gene transfer kinetics and efficiency. This novel ex vivo LV transduction platform is flexible in design, easy to use, scalable, and compatible with standard cell transduction reagents and LV preparations. Using hematopoietic cell lines, primary human T cells, primary hematopoietic stem and progenitor cells (HSPCs) of both murine (Sca-1+) and human (CD34+) origin, microfluidic transduction using clinically processed LVs occurs up to 5-fold faster and requires as little as one-twentieth of LV. As an in vivo validation of the microfluidic-based transduction technology, HSPC gene therapy was performed in hemophilia A mice using limiting amounts of LV. Compared to the standard static well-based transduction protocols, only animals transplanted with microfluidic-transduced cells displayed clotting levels restored to normal.
On-Table Endoscopy: A Simple Effective Quality Assurance Measure in Laparoscopic Anti-Reflux Surgery
Authors: N Ravi, N Al-Sarraf, PJ Byrne, JV Reynolds
Journal: Endoscopy (2006)

Objective: To assess prospectively the impact of on-table endoscopy at laparoscopic Rosetti-Nissen fundoplication to qualitative improvement in outcomes.
A CONCEPT MAPPING APPROACH TO IDENTIFY PERCEIVED FACILITATORS TO ENHANCE THE IMPLEMENTATION OF INJURY PREVENTION TRAINING IN YOUTH TEAM HANDBALL: THE I-PROTECT PROJECT
Authors: Eva Ageberg, Sofia Bunke, Karolina Andersson, Per Nilsen, Alex Donaldson
Journal: British Journal of Sports Medicine (2017)

Background Evidence-based injury preventive exercise programs (IPEPs) usually have limited public health impact because they are not widely implemented into regular training routines. Objective To identify facilitators that could help make IPEPs part of regular training routines in youth team handball. Design Ecological participatory approach incorporating the perspective of multiple stakeholders. This is the first phase in a series of studies within the “Implementing injury Prevention exercise ROutines in TEams and Clubs in youth Team handball (I-PROTECT)” project. Setting Two community team handball clubs in Sweden offering organized training for youth players. Participants Stakeholders from multiple levels of youth handball. Methods Concept Mapping (CM), a mixed-method approach for qualitative data collection and quantitative data analysis, was used. CM involves steps from generating statements from stakeholders to conceptualizing a framework for developing an implementation plan. Results 179 stakeholders (32% players, 14% coaches, 42% caregivers, and 12% club, district and national handball administrators) participated in a brainstorming in response to the question “ In order to make injury prevention training a part of our regular handball training routines I need….” . This generated 89 unique ‘facilitator’ statements which are currently being sorted into clusters and rated for ‘ importance ’ and ‘ feasibility ’ by 35 stakeholders. To date, data collection is completed for 11 of 35 stakeholders, yielding 5 clusters (stress value 0.255): “Understanding and motivation”, “Education, knowledge and consistency”, “Exercises and set-up”, “Routines and quality assurance”, and “Club policy and expert collaboration”. Statements rated as both highly important and feasible indicate that developing an IPEP and an associated implementation plan in partnership with all stakeholders should be a high priority. Conclusions The preliminary results suggest that developing an evidence-based context-specific IPEP and an associated context-specific implementation plan in partnership with all stakeholders should be a high priority to ensure successful IPEP implementation in youth team handball.
Association of Centers for Medicare &amp; Medicaid Services Overall Hospital Quality Star Rating With Outcomes in Advanced Laparoscopic Abdominal Surgery
Authors: Christina Y. Koh, Colette S. Inaba, Sarath Sujatha-Bhaskar, Ninh T. Nguyen
Journal: JAMA Surgery (2017)

<h3>Importance</h3> The Centers for Medicare &amp; Medicaid Services (CMS) recently released the Overall Hospital Quality Star Rating to help patients compare hospitals based on a 5-star scale. The star rating was designed to assess overall quality of the institution; thus, its validity toward specifically assessing surgical quality is unknown. <h3>Objective</h3> To examine whether CMS high-star hospitals (HSHs) have improved patient outcomes and resource use in advanced laparoscopic abdominal surgery compared with low-star hospitals (LSHs). <h3>Design, Setting, and Participants</h3> Using the University HealthSystem Consortium database (which includes academic centers and their affiliate hospitals) from January 1, 2013, through December 31, 2015, this administrative database observational study compared outcomes of 72 662 advanced laparoscopic abdominal operations between HSHs (4-5 stars) and LSHs (1-2 stars). The star rating includes 57 measures across 7 areas of quality. Patients who underwent advanced laparoscopic abdominal surgery, including bariatric surgery (sleeve gastrectomy, Roux-en-Y gastric bypass), colorectal surgery (colectomy, proctectomy), or hiatal hernia surgery (paraesophageal hernia repair, Nissen fundoplication), were included. Risk adjustment included exclusion of patients with major and extreme severity of illness. <h3>Main Outcomes and Measures</h3> Main outcome measures included serious morbidity, in-hospital mortality, intensive care unit admissions, and cost. <h3>Results</h3> A total of 72 662 advanced laparoscopic abdominal operations were performed in patients at 66 HSHs (n = 38 299; mean [SD] age, 51.26 [15.25] years; 12 096 [31.5%] male and 26 203 [68.4%] female; 28 971 [75.6%] white and 9328 [24.4%] nonwhite) and 78 LSHs (n = 34 363; mean [SD] age, 49.77 [14.77] years; 9902 [28.8%] male and 24 461 [71.2%] female; 21 876 [67.6%] white and 12 487 [32.4%] nonwhite). The HSHs were observed to have fewer intensive care unit admissions (1007 [2.6%] vs 1711 [5.0%],<i>P</i> &lt; .001) and lower mean cost ($7866 vs $8708,<i>P</i> &lt; .001). No significant difference was found in mortality between HSHs and LSHs for any advanced laparoscopic abdominal surgery. No significant difference was found in serious morbidity between HSHs and LSHs for bariatric or hiatal hernia surgery. However, for colorectal surgery, serious morbidity was lower at HSHs compared with LSHs (258 [2.2%] vs 276 [2.9%],<i>P</i> = .002). <h3>Conclusions and Relevance</h3> This study found that HSHs treat fewer ethnic minorities and have similar outcomes as LSHs for advanced laparoscopic abdominal operations. However, HSHs may represent hospitals with improved resource use and cost.
Cardiovascular Disease Does Not Predict Exacerbation Rate or Mortality in Chronic Obstructive Pulmonary Disease
Authors: Paul W. Jones, Hana Mullerova, Alvar Agusti, Marc Decramer, Lukasz Adamek, Alice Raillard, Chang-Qing Zhu, Jadwiga A Wedzicha
Journal: American Journal of Respiratory and Critical Care Medicine (2017)
Is antibody to surface antigen associated with hepatitis B reactivation in patients with resolved hepatitis B?
Authors: Yi‐Hsiang Huang, Shou‐Dong Lee
Journal: Hepatology (2017)

Potential conflict of interest: Nothing to report. To the editor: In an issue of Hepatology, Paul et al. reported an interesting meta‐analysis study that showed that the presence of antibodies to hepatitis B surface antigen (HBsAg, anti‐HBs) is associated with a decreased risk of hepatitis B virus reactivation (HBVr) in patients with resolved hepatitis B undergoing chemotherapy.1 However, in this meta‐analysis, some points should be further clarified. First, the definition of HBVr for resolved hepatitis B was not consistent in previous reports and included criteria such as HBsAg reverse seroconversion, a detectable HBV viral load from a previously undetectable status, elevation of the HBV viral load to 2,000 IU/mL, or a 10‐fold in increase in HBV DNA. In our previous randomized controlled trial (RCT), an elevation of HBV viral load to 2,000 IU/mL was adopted as the threshold of HBVr.2 However, Paul et al. incorrectly classified the HBVr definition of our RCT as detectable HBV DNA. In our RCT, the baseline HBV DNA levels of the seven cases of HBVr in the control arm ranged from undetectable (<12 IU/mL) to 61 IU/mL. This indicated that all the patients had more than a 10‐fold increase in HBV viral load during HBVr, instead of only detectable HBV DNA.2 Second, the incidence of HBVr (HBV DNA elevation) is higher than that of HBsAg reverse seroconversion during rituximab treatment, even in the presence of anti‐HBs (Fig. 1). Based on our RCT, 4 patients positive for anti‐HBs (with titers ranging from 21 to > 1,000 mIU/mL) had HBVr in the control arm. The patient with the highest titer of anti‐HBs (>1,000 mIU/mL) developed HBVr with an increase in the HBV viral load to 46,300 IU/mL. Another study also showed a high risk of HBVr (21%) in anti‐HBs‐positive patients undergoing rituximab treatment.3 The information provided by this meta‐analysis seems to have overlooked the risk of HBVr in anti‐HBs‐positive lymphoma patients undergoing rituximab‐based chemotherapy. Third, the incidence of reactivation depends on the criteria of HBVr. In our RCT, the difference between the incidence of HBVr and HBsAg reverse seroconversion was 7.4% (17.9% versus 10.3%).2 The risk and odds ratio of HBVr in this meta‐analysis might be distorted by mixing all types of HBVr definitions together. The data should be precisely presented by grouping the enrolled studies according to their definition of HBVr.Figure 1: Risk of HBVr stratified by anti‐HBs status (modified from Huang et al.2). Abbreviation: HBc, hepatitis B core.
Symbiotic Consumption Reduces the Proliferative Activity of the Colonic Mucosa but does not Influence the Tumour Promoting Activity of Faecal Water (FW)
Authors: Y Clune, G Caderni, M O'Riordan, G O'Sullivan, J Collins
Journal: Endoscopy (2006)

Aims: The aim of this study was to determine if symbiotic consumption could influence the proliferative activity of the colonic mucosa, the tumour promoting potential of faecal water (FW), the expression of glutathione-S transferases (GSTs) and the levels of CEA and CA 19–9. Synbiotic consumption significantly reduced the proliferative activity in biopsies from polyp subjects and increased GST activity in cancer subjects. There was no change in the tumour promoting activity of FW as measured by comet assay. Neither was there an effect of synbiotic consumption on the levels of CEA and CA 19–9. The influence of FW on tight junction integrity in Caco-2 cells was significantly different for FW derived from polyp subjects compared to FW from cancer subjects indicating that the composition of FW is different in these two groups. In conclusion synbiotic consumption influences the proliferative activity of the colonic mucosa and the expression of detoxifying enzymes in a health promoting manner. The composition of FW derived from cancer subjects is different to FW from polyp subjects at the beginning of intervention and at all time points. Proliferative activity, GST expression and the influence of FW on tight junction integrity may be useful as biomarkers of cancer risk.
Abstract P103: Genome-Wide Study of Gene Variants Associated with Differential Event Reduction by Pravastatin Therapy
Authors: Dov Shiffman, Stella Trompet, Judy Z Louie, Charles M Rowland, Joseph J Catanese, Olga A Iakoubova, Todd G Kirchgessner, David J Scott, Naveed Sattar, James J Devlin, Christopher J Packard, Ian Ford, Frank M Sacks, J W Jukema
Journal: Circulation Research (2011)

BACKGROUND : Statin therapy reduces the risk of coronary heart disease (CHD); however, the variability in response to statin therapy is not well understood. We investigated the effect of genetic variation on the reduction of CHD events by pravastatin therapy. METHODS : We genotyped 682 CHD cases from CARE and 383 CHD cases from WOSCOPS, two randomized placebo-controlled studies of pravastatin using the Illumina OMNI1 bead array. Single nucleotide polymorphisms (SNPs) that were associated with differential CHD event reduction by pravastatin therapy were investigated in PROSPER, a randomized placebo-controlled study of pravastatin in the elderly. RESULTS : A combined case-only analysis of CARE and WOSCOPS identified 62 SNPs associated with differential event reduction by pravastatin therapy (P int &lt;0.0001 for interaction between treatment and genotype in an additive model). We investigated 57 of these SNPs in PROSPER. In an analysis that included cases as well as non-cases of CARE, WOSCOPS, and PROSPER, we found that for an intronic SNP in DNAJC5B (rs13279522), CHD event reduction by pravastatin therapy according to genotype differed in all 3 studies: P int =0.001 in CARE, P int =0.01 in WOSCOPS, P int =0.002 in PROSPER, and P int =3X10 -7 in a combined analysis of CARE, WOSCOPS and PROSPER (Figure). CONCLUSIONS : We have identified a SNP that is associated with differential event reduction by pravastatin therapy in CARE, WOSCOPS, and PROSPER. This SNP merits investigation in additional randomized studies of pravastatin and other statins.
Exceptional requests for care surge as rationing deepens
Authors: Gareth Iacobucci
Journal: BMJ (2017)

The number of exceptional funding requests that GPs in England are making on behalf of their patients for treatments such as cataract removal, hip and knee replacements, and mental health interventions has increased markedly, an investigation by The BMJ has found.1

Richard Vautrey, a GP in Leeds and deputy chair of the BMA’s General Practitioners Committee, said that the figures were a sign that clinical commissioning groups were under increasing financial constraint. “There is undoubtedly more pressure on CCG budgets and attempts to reduce referrals and costs, and the greater use of IFRs [individual funding requests] …
ID1 enhances docetaxel cytotoxicity in prostate cancer cells through inhibition of p21 (Cancer Research (2010) 70, (3239-3248) DOI
Authors: H. Geng, B. L. Rademacher, J. Pittsenbarger, C. Y. Huang, C. T. Harvey, M. C. Lafortune, A. Myrthue, M. Garzotto, P. S. Nelson, T. M. Beer, D. Z. Qian
Journal: Cancer Research (2010)
Abstract 220: miR-486 Mediates the Benefits of Exercise in Attenuating Cardiac Fibrosis
Authors: Dongchao Lv, Yihua Bei, Qiulian Zhou, Qi Sun, Tianzhao Xu, Junjie Xiao
Journal: Circulation Research (2015)

MicroRNAs (miRNAs, miRs), a novel group of small non-coding RNAs, play important roles in cardiac fibrosis. Exercise-induced physiological cardiac growth is associated with hypertrophy and proliferation of cardiomyocytes. In addition, exercise has been shown to inhibit cardiac fibrosis. However, relative little is known about whether exercise could attenuating cardiac fibrosis via targeting miRNA. miR-486 is a muscle enriched miRNAs, however, its role in heart is relative unclear. The current study aimed to investigate the role of miR-486 in exercise-induced cardiac growth in a 3-week swimming training murine model as well as in the function of cardiac fibroblasts and production of extracellular matrix (ECM) using neonatal rat cardiac fibroblasts in primary culture. Our data showed that exercised mice displayed increased about three-fold expression of miR-486 in hearts as measured by microarray analysis and qRT-PCRs. EdU proliferation assays demonstrated that miR-486 mimics decreased (5.90%±0.57% vs 4.02%±0.27% in nc-mimics vs miR-486-mimics, respectively), while miR-486 inhibitor increased the proliferation of cardiac fibroblasts in vitro (5.87%±0.16% vs 9.60%±0.58% in nc-inhibitor vs miR-486-inhibitor, respectively). Although downregulation of miR-486 had no regulatory effect on α-sma and collagen-1 gene expression in cardiac fibroblasts, overexpression of miR-486 significantly reduced the mRNA level of α-sma (1.01±0.08 vs 0.28±0.04 in nc-mimics vs miR-486-mimics, respectively) and collagen-1(1.02±0.12 vs 0.58±0.09 in nc-mimics vs miR-486-mimics, respectively), indicative of attenuated activation of fibroblasts and reduced production of ECM. These data reveal that miR-486 is essentially involved in the proliferation and activation of cardiac fibroblasts, and might be a key regulator mediating the benefit of exercise in preventing cardiac fibrosis.
FOLFOX/Bevacizumab +/− Irinotecan in advanced colorectal cancer (AIO) “CHARTA”: Final results and multivariate prognostic factor analysis
Authors: Hans-Joachim Schmoll, Benjamin Garlipp, Christian Junghanß, Malte Leithäuser, Arndt Vogel, Michael Schaefers, Ulrich Kaiser, Heinz-Gert Hoeffkes, Axel Florschütz, Jörn Rüssel, Stephan Kanzler, Thomas Edelmann, Helmut Forstbauer, Thomas Göhler, Carla Hannig, Bert Hildebrandt, Jörg Steighardt, Franziska Cygon, Fabian Meinert, Alexander Stein
Journal: Annals of Oncology (2017)
Characterization of Bleeding in Hemophilia Carriers and Comparison to Women with Type 1 Von Willebrand Disease, Type 3 Von Willebrand Disease Obligate Carriers and Controls
Authors: Natasha Amrita Satkunam, Johnny Mahlangu, Christoph Bidlingmaier, Maria Eva Mingot-Castellano, Meera B. Chitlur, Patrick F. Fogarty, Adam Cuker, Maria Elisa Mancuso, Pal André Holme, Julie Grabell, Wilma Hopman, Prasad Mathew, Paula D. James
Journal: Blood (2016)
Erratum: Copy number analysis indicates monoclonal origin of lethal metastatic prostate cancer
Authors: Wennuan Liu, Sari Laitinen, Sofia Khan, Mauno Vihinen, Jeanne Kowalski, Guoqiang Yu, Li Chen, Charles M Ewing, Mario A Eisenberger, Michael A Carducci, William G Nelson, Srinivasan Yegnasubramanian, Jun Luo, Yue Wang, Jianfeng Xu, William B Isaacs, Tapio Visakorpi, G Steven Bova
Journal: Nature Medicine (2009)
Botulinum toxin a for adolescents with persisting post-concussion headaches: a case series
Authors: Jacqueline Purtzki, Catherine Chan
Journal: British Journal of Sports Medicine (2017)

Objective To present safety and tolerability of treating persistent post-concussive headaches with botulinum toxin A (Botox) in adolescents. Design Retrospective case series. Setting A tertiary rehabilitation hospital interdisciplinary outpatient concussion clinic for adolescents aged 12–18 years. Participants Seven patients ages 15–18 with persistent post-concussion headaches. Interventions 1. Comprehensive assessment by an interdisciplinary rehab team. 2. Establishing the diagnosis of chronic daily headache disorder with 15 headaches per month, 8 of them meeting pediatric migraine criteria. Headache treatment included neck exercises, physiotherapy, CBT, mindfulness, oral prophylactic and abortive migraine medications as well as Botox. Patients selected had prolonged debilitating headaches, had failed other treatment options, and/or had specific circumstances precluding use of other treatment options, were unable to attend school, tolerate daily activities. 3. Discussion of potential side effects of Botox, complications of injection, review of current literature evidence. Verbal consent obtained. Clinical monitoring of adverse events of injection, side effects of Botox, effect on daily activities and headaches. Main results There were no adverse events related to the injection of Botox. Some patients experienced a transient flare up of headaches after injection. All patients reported that injections and post-injection side effects were tolerable. Reduction in headaches was variable. Conclusions Based on this small clinical sample of adolescents with severe persistent post-concussion headaches, Botox appears to be a well-tolerated and safe treatment option. Prospective, RCT studies are required to confirm this preliminary clinical impression. Competing interests None.