tbuckley/cabot-search huggingface dataset:
Abstract 12857: Echocardiographic Patterns of Post-cardiac Arrest Myocardial Dysfunction
Authors: Kyoung-Chul Cha, Sung Oh Hwang, Hyung Il Kim, Oh Hyun Kim, Yong Sung Cha, Hyun Kim, Kang Hyun Lee
Journal: Circulation (2016)

Purpose: Post-cardiac arrest myocardial dysfunction can be developed after successful resuscitation from cardiac arrest. However, echocardiographic patterns of post-cardiac arrest myocardial dysfunction are not yet known. The purpose of this study was to investigate the manifestations of post-cardiac arrest myocardial dysfunction with serial echocardiography during post-cardiac arrest period. Materials and Methods: We enrolled non-traumatic out-of-hospital cardiac arrest patients older than 18 years old who were successfully resuscitated after CPR. The patients with myocardial infarction and pre-existing cardiac disease including heart failure or myocardial disease were excluded. Transthoracic echocardiography was performed within 24 hours, between 24 to 48 hours, after 48 hour since restoration of spontaneous circulation (ROSC) was achieved. Results: Out of 263 patients, 186 patients (122 males) were enrolled in the analysis. No left ventricular (LV) dysfunction was observed in 120 patients (64.5%). Abnormal LV dysfunction was observed in 66 patients (35.5%). Abnormal LV dysfunctions included global hypokinesis in 32 patients (17.2%), regional wall motion abnormalities (RWMA) in 25 patients (13.4%), and abnormalities like stress-induced cardiomyopathy in 9 patients (4.8%). There were no differences in etiology (p=0.275), duration of cardiac arrest (p=0.078), cumulative dose of epinephrine (p=0.336), and hospital mortality (p=0.140) according to the pattern of post-cardiac arrest myocardial dysfunction. In subgroup analysis of the patients with shockable rhythm, cumulative defibrillation energy (joules) was lower in patients without LV dysfunction (101±41) than in patients with RWMA (200±74) or global hypokinesis (408±131) (p=0.011). Cut-off value of 220 J had 21% of sensitivity and 91% of specificity for development of LV dysfunction (AUC=0.572). Conclusion: Post-cardiac arrest myocardial dysfunction occurs in one thirds of patients resuscitated from cardiac arrest. Echocardiographic patterns of post-cardiac arrest LV dysfunction include global hypokinesis, regional wall motion abnormalities, and abnormalities like stress-induced cardiomyopathy.
Abstract 4390: Developing methods for investigating cold atmospheric plasma effects on cervical cancer
Authors: Nicole J. Sova, Yonry Zhu, Quinn O. Mitchell, Ariel L. Lanier, David Burnette, Monica M. Burdick
Journal: Cancer Research (2018)

Abstract Cold atmospheric plasma (CAP) has been suggested for use as a novel cancer therapeutic due to its reported ability to induce cell-specific death. Cervical cancer is the fourth most common cancer in women. Its prevalence and accessibility for topical treatment make cervical cancer an ideal target for CAP treatments. Despite its potential impact, effects of CAP in biological systems are not well understood. To better understand the viability of CAP as a therapy, this project investigated in vitro cellular responses induced by CAP treatment within cancerous cervical cells (SiHa) and non-cancerous cervical (Ect1/E6E7 and End1/E6E7) and vaginal cells (VK2/E6E7). Plasma is a state of ionized gas, produced using a controlled electric field or extreme heat. CAP, however, is a subset of plasma that is produced near 1 atm and 40oC. This allows for CAP treatment of biological systems without heating-induced effects. A dielectric barrier discharge plasma pen was developed for this study. Plasma modeling was conducted in order to predict the reactive species present in the system. CAP treatments were performed on cells for 30 seconds using a helium carrier gas at a flowrate of 1 L/min and a DC power supply with input settings of 10kV, 1µs, and 0.1A. Cell vitality assays demonstrated induced cell death following exposure to CAP treatment in both cancerous and noncancerous cervical cells as indicated by flow cytometric analysis and florescence microscopy. CAP treatment induced cell death in 76 ± 8% of treated cancerous, SiHa, cells. In comparison, noncancerous cell lines Ect1/E6E7, End1/E6E7, and VK2/E6E7 underwent 49 ± 8%, 42 ± 13%, and 51 ± 7% cell death, respectively. Flow cytometry characterization and wound healing assays indicate that CAP treatment does not significantly affect the expression of investigated cell surface adhesion molecules or cell migration. Cells were treated with helium carrier gas as the negative control and hydrogen peroxide as an induced death control. Altered levels and locations of uptake of FITC and quantum dots following CAP treatment suggest modification of cell membranes as compared to carrier gas treatment controls. Adjustments in membrane permeability may be a mechanism through which CAP treatment increases exposure to apoptotic triggers. In conclusion, comprehensive understanding of CAP effects on cancerous and non-cancerous cells could lead to the development of effective CAP-based therapies for cervical cancer. Citation Format: Nicole J. Sova, Yonry Zhu, Quinn O. Mitchell, Ariel L. Lanier, David Burnette, Monica M. Burdick. Developing methods for investigating cold atmospheric plasma effects on cervical cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 4390.
The Prognostic Value of 18F-FDG PET/CT in Monitoring Chemotherapy in Ovarian Cancer Both at Initial Diagnosis and at Recurrent Disease
Authors: Domenico Rubello, Maria Cristina Marzola, Patrick M. Colletti
Journal: Clinical Nuclear Medicine (2018)

Published experiences related to the prognostic relevance of negative or positive FDG PET/CT in patients treated for ovarian cancer for progression-free survival and overall survival are typically heterogeneous retrospective analyses. Several points have been well defined, these are as follows: (a) there is a correlation between FDG tumor uptake and prognosis; (b) ovarian cancer patients treated by neoadjuvant or adjuvant chemotherapy can be divided into responders with reduction in tumor FDG uptake during and after treatment and nonresponders where tumor FDG uptake remains stable or increases after treatment;
Abstract 989: Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages and can form chimeric mammary glands in vivo
Authors: Jennifer M. Rosenbluth, Ronald Schackmann, Carman Li, Norman Sachs, Deborah Dillon, Andrea Richardson, Jane Brock, Judy Garber, Gary Kenneth Gray, Jason Zoeller, Mackenzie Boedicker, Hendrick Johannes Kuiken, Hans Clevers, Joan Brugge
Journal: Cancer Research (2018)

Abstract Use of cell culture models to investigate the development of cancer associated with familial breast cancer syndromes is challenging because many culture methodologies do not maintain all of the lineages that are present in vivo, particularly the progenitor cells that may be the specific cancer cell-of-origin. We evaluated the ability of organoid culture technology to preserve stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Tissue samples were obtained from elective reduction mammoplasties or from prophylactic mastectomies for cancer prevention, and were histologically normal. These were grown using conditions similar to organoid cultures for other epithelial cell types, as has been recently described (Sachs et al., Cell 2018). We found that basal stem and luminal progenitor cells could be cultured long-term, and differentiated in culture to generate mature basal and luminal cell types. Analysis of matched organoid cultures and native tissues by mass cytometry (CyTOF) for 38 markers at single-cell resolution confirmed the presence of multiple mammary epithelial cell types in the organoids, and demonstrated that protein expression patterns of the tissue of origin were largely preserved in culture. In addition, we generated a panel of over 40 mammary organoids derived from patients harboring inherited mutations in the cancer predisposition gene BRCA1 and from unaffected controls. Despite interindividual variability, an expansion in luminal progenitor populations associated with BRCA1 mutation could be detected in cultured organoids. Human mammary organoids heterozygous for BRCA1 could be engrafted into the murine mammary gland, resulting in mixed ductal and acinar structures. These studies indicate that this new model system is well suited for studies of aberrant phenotypes associated with BRCA1 mutation and approaches to prevent cancer development in these genetically predisposed tissues. Citation Format: Jennifer M. Rosenbluth, Ronald Schackmann, Carman Li, Norman Sachs, Deborah Dillon, Andrea Richardson, Jane Brock, Judy Garber, Gary Kenneth Gray, Jason Zoeller, Mackenzie Boedicker, Hendrick Johannes Kuiken, Hans Clevers, Joan Brugge. Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages and can form chimeric mammary glands in vivo [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 989.
What do Vitiligo Impact Scale‐22 scores mean? Studying the clinical interpretation of scores using an anchor‐based approach
Authors: V. Gupta, V. Sreenivas, M. Mehta, M. Ramam
Journal: British Journal of Dermatology (2018)

The Vitiligo Impact Scale (VIS)‐22 is a vitiligo‐specific quality‐of‐life instrument. Its criterion, convergent and known‐groups validity, test–retest reliability and responsiveness have been studied previously in an Indian population. The clinical meaning of VIS‐22 scores has not yet been analysed. To assign clinical meaning to VIS‐22 scores using anchor‐based methods. This was a cross‐sectional study conducted in a large teaching hospital in North India. Patients with vitiligo > 15 years of age (n = 391) completed the VIS‐22 and Dermatology Life Quality Index (DLQI) questionnaires, and answered a Global Question (GQ) on the effect of vitiligo on their lives on a five‐point Likert scale. Multiple band sets of VIS‐22 scores were devised using GQ as the anchor. A weighted kappa‐coefficient was calculated to estimate the level of agreement between different band sets of VIS‐22 and GQ. VIS‐22 and DLQI were compared based on their degree of correlation and agreement with GQ. The mean ± SD of VIS‐22 scores was 24·8 ± 14·0 (range 0–61). VIS‐22 scores showed good correlation with GQ (r = 0·76). Of the various VIS‐22 band sets tested, the following was chosen: 0–5, 6–15, 16–25, 26–40 and 41–66 (weighted κ = 0·57), corresponding to the five categories of GQ. The degree of correlation (VIS‐22, r = 0·77; DLQI, r = 0·69) and agreement (VIS‐22, 51·6%; DLQI, 36·1%; P < 0·001) of VIS‐22 with GQ was higher than that with DLQI. VIS‐22 scores can be used to stratify the impairment of vitiligo‐related quality of life.
Role of Protein Kinase C in Mitochondrial KATP Channel–Mediated Protection Against Ca2+ Overload Injury in Rat Myocardium
Authors: YigangWang, MuhammadAshraf
Journal: Circulation Research (1999)

Abstract—Growing evidence exists that ATP-sensitive mitochondrial potassium channels (MitoKATP channel) are a major contributor to the cardiac protection against ischemia. Given the importance of mitochondria in the cardiac cell, we tested whether the potent and specific opener of the MitoKATP channel diazoxide attenuates the lethal injury associated with Ca2+overload. The specific aims of this study were to test whether protection by diazoxide is mediated by MitoKATP channels; whether diazoxide mimics the effects of Ca2+ preconditioning; and whether diazoxide reduces Ca2+ paradox (PD) injury via protein kinase C (PKC) signaling pathways. Langendorff-perfused rat hearts were subjected to the Ca2+ PD (10 minutes of Ca2+ depletion followed by 10 minutes of Ca2+ repletion). The effects of the MitoKATP channel and other interventions on functional, biochemical, and pathological changes in hearts subjected to Ca2+ PD were assessed. In hearts treated with 80 μmol/L diazoxide, left ventricular end-diastolic pres...
Abstract 4024: TRAF2 Ser-11 phosphorylation confers resistance to AKT inhibitors in breast cancer cells
Authors: Hasem Habelhah, Laiqun Zhang
Journal: Cancer Research (2018)

Abstract The PI3K-AKT pathway is the most frequently aberrantly activated pathway in breast cancer (BC), with alterations in genes encoding the pathway components (e.g. RTKs, HER2, PTEN, PIK3C and AKT) occurring in ~80% of cases. However, inhibition of this pathway is either not effective or often results in development of resistance in BC. TRAF2 is a key effector protein downstream of the TNF receptor superfamily, and activates NF-κB to promote BC cell survival. We mapped two phosphorylation sites (Ser-11 and Ser-274) in TRAF2, and identified TBK1/IKKϵ and AKT as the kinases that directly phosphorylate TRAF2 at Ser-11 and Ser-274, respectively. TBK1 and IKKϵ are serine/threonine kinases activated by inflammatory stimuli and the oncogenic RTK-Ras-RALB pathway, and play a critical role in BC transformation and survival. IKKϵ and TRAF2 are overexpressed in a significant portion of BC, and their overexpression is associated with poor prognosis in patients with invasive breast carcinoma. We found that inhibition of AKT leads to increased TBK1 activation and TRAF2 Ser-11 phosphorylation in triple negative BC (TNBC) cells. Importantly, combination of AKT and TBK1/IKKϵ inhibitors synergistically induced apoptosis in all TNBC cell lines tested in vitro, suggesting that inhibition of PI3K-AKT derepresses the pro-survival TBK1-TRAF2 pathway, which in turn confers resistance to AKT inhibition in TNBC. We believe that any positive outcomes from our ongoing xenograft experiments will have an immediate translational impact in TNBC treatment. Citation Format: Hasem Habelhah, Laiqun Zhang. TRAF2 Ser-11 phosphorylation confers resistance to AKT inhibitors in breast cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 4024.
Angiogenic Effects of Adrenomedullin in Ischemia and Tumor Growth
Authors: SatoshiIimuro, TakayukiShindo, NobuoMoriyama, ToshihiroAmaki, PeiNiu, NorifumiTakeda, HiroshiIwata, YuelanZhang, AyaEbihara, RyozoNagai
Journal: Circulation Research (2004)

Adrenomedullin (AM) is a novel vasodilating peptide involved in the regulation of circulatory homeostasis and implicated in the pathophysiology of cardiovascular disease. We tested the hypothesis that AM also possesses angiogenic properties. Using laser Doppler perfusion imaging, we found that AM stimulated recovery of blood flow to the affected limb in the mouse hind-limb ischemia model. AM exerted this effect in part by promoting expression of vascular endothelial growth factor (VEGF) in the ischemic limb, and immunostaining for CD31 showed the enhanced flow to reflect increased collateral capillary density. By enhancing tumor angiogenesis, AM also promoted the growth of subcutaneously transplanted sarcoma 180 tumor cells. However, heterozygotic AM knockout mice (AM+/−) showed significantly less blood flow recovery with less collateral capillary development and VEGF expression than their wild-type littermates. Similarly, mice treated with AM22-52, a competitive inhibitor of AM, showed reduced capillary ...
Specific Regulation of Noncanonical p38α Activation by Hsp90-Cdc37 Chaperone Complex in Cardiomyocyte
Authors: AsukaOta, JunZhang, PeipeiPing, JiahuaiHan, YibinWang
Journal: Circulation Research (2010)

Rationale: p38 is an important stress activated protein kinase involved in gene regulation, proliferation, differentiation, and cell death regulation in heart. p38 kinase activity can be induced through canonical pathway via upstream kinases or by noncanonical autophosphorylation. The intracellular p38 kinase activity is tightly regulated and maintained at low level under basal condition. The underlying regulatory mechanism for canonical p38 kinase activation is well-studied, but the regulation of noncanonical p38 autophosphorylation remains poorly understood. Objective: We investigated the molecular basis for the regulation of noncanonical p38 autophosphorylation and its potential functional impact in cardiomyocytes. Methods and Results: Using both proteomic and biochemical tools, we established that heat shock protein (Hsp)90-Cdc37 chaperones are part of the p38α signaling complex in mammalian cells both in vitro and in vivo. The Hsp90-Cdc37 chaperone complex interacts with p38 via direct binding betwee...
POEMS症候群(Crow‐Fukase症候群)に合併した肺高血圧症の1症例
Authors: 大坂友美子, 櫻井馨, 田尾進, 大西健太郎, 佐々木毅, 稲垣裕, 川端美穂子, 蜂谷仁, 鈴木淳一, 畑明宏, 平尾見三, 磯部光章
Journal: Circulation (2007)
Abstract 206: Utilization and Outcome of Therapeutic Hypothermia for Out-of-Hospital Cardiac Arrest
Authors: AwaniDeshmukh, AchintPatel, NileshkumarPatel, AbhishekDeshmukh, NeilPatel, AvirupGuha, GrishmaDhaduk, SadipPant, ShantanuSolanki, SohilkumarManvar, ShilpkumarArora, NilayPatel, VinaykumarPanchal, BadalThakkar, ApurvaBadheka, HakanPaydak, ManishJoshi
Journal: Circulation (2014)

Background: Therapeutic hypothermia (TH) is recommended in international resuscitation guidelines.TH has been incorporated into the American Heart Association practice guidelines and use of TH has steadily increased, but widespread clinical uptake remains low Hypothesis: Our aim was to examine the frequency of TH use after cardiac arrest in United States. Methods: We analyzed data from years 2004-2011 from the United States Nationwide Inpatient Sample which is the largest hospitalization database available in United States. Using International Classification of Diseases-9 (ICD-9) codes, we identified cases with cardiac arrest (427.5) who underwent therapeutic hypothermia (99.81). Population estimates were obtained by complex sample analysis used for NIS design. Results: The utilization of TH in OHCA has significantly increased from 0.06% in 2004 to 6.43 % in 2011. The TH was utilized more in men (53.5%), age group 65-79 years (35.0%), white ( 59.3% ) , private insurance ( 52.8% ) , charlsons comorbidity i...
Short Communication: Dating Components of Human Atherosclerotic Plaques
Authors: IsabelGonçalves, KristinaStenström, GöranSkog, SörenMattsson, MihaelaNitulescu, JanNilsson
Journal: Circulation Research (2010)

Rationale: Atherosclerotic plaques that give rise to acute clinical symptoms are typically characterized by degradation of the connective tissue and plaque rupture. Experimental studies have shown that mechanisms to repair vulnerable lesions exist, but the rate of remodeling of human plaque tissue has not been studied. Objective: In the present study, we determined the biological age of different components of advanced human atherosclerotic plaques by analyzing tissue levels of 14C released into the atmosphere during the nuclear weapons tests in the late 1950s and early 1960s. Methods and Results: Atherosclerotic plaques were obtained from 10 patients (age 46 to 80 years) undergoing carotid surgery. Different regions of the plaques were dissected and analyzed for 14C content using accelerator mass spectrometry. At the time of surgery, the mean biological age of the cap region was 6.4±3.2 years, which was significantly lower than that of the shoulder region (12.9±3.0 years, P<0.01), the interface toward th...
Abstract 343: Activation of ROR-α, but not ROR-β or ROR-γ, Protects against Myocardial Ischemia/Reperfusion Injury
Authors: JunPu, BenHe, ErheGao, XinliangMa, YajingWang
Journal: Circulation Research (2014)

Objectives: The RAR-related orphan receptors (RORs) are members of the nuclear receptor superfamily that play a pivotal role in many physiological processes, including regulation of the circadian rhythm, development, metabolism and immune function. Three different but highly homologous ROR isoforms, ROR-α, -β, and -γ, have been discovered separately. However, the functional roles of RORs in the heart have never been investigated. We investigate the role of RORs in the pathophysiology of acute myocardial ischemia/ reperfusion (MI/R) injury. Methods and Results: The endogenous RORα, but not RORβ or RORγ, was significantly upregulated after MI/R. Synthetic ROR agonists SR1078 and SR3335 reduced myocardial infarction and improved contractile function after MI/R. Mechanistically, ROR activation inhibited endoplasmic reticulum (ER) stress, attenuated mitochondrial impairment, reduced cardiomyocyte apoptosis, and inhibited MI/R-induced autophagy dysfunction. Moreover, ROR activation inhibited MI/R-induced oxidat...
Abstract 18145: Non-cardiac Surgery in Patients With a Ventricular Assist Device
Authors: Juliane Vierecke, Thomas Krabatsch, Martin Schweiger, Alexander Stepanenko, Evgenji Potapov, Roland Hetzer
Journal: Circulation (2012)

Introduction As both VAD support duration and patient age increase, there is greater likelihood that some of these patients will develop general surgical problems and require non-cardiac surgery (NCS). The clinical course of patients requiring NCS during VAD support is not well described. Previous studies are limited in the number of patients with VADs and number of operations. Methods We reviewed the NCS procedures of our 190 patients with ongoing VAD support between 10/2010 and 10/2011. NCS was divided into two groups: VAD-related NCS and non-VAD-related NCS. Results Fifty of our patients underwent 52 NCS procedures in 1 year. We documented 16 VAD-related NCS and 36 non-VAD-related NCS; 14 of these procedures were urgent and 38 were elective. Median age at time of NCS was 58 years; 92% of these patients were male and 52% had ischemic cardiomyopathy. Different devices were used: HeartWare 52%, HeartMate II 32%, INCOR 12%, Berlin Heart BVAD 2%, HW BVAD 2%. One third of the non-VAD-related NCS were dental ...
Acute Rupture of Chordae Tendineae of the Mitral Valve in Infants
Authors: IsaoShiraishi, KunihiroNishimura, HeimaSakaguchi, TadaakiAbe, MasatakaKitano, KenichiKurosaki, HitoshiKato, ToshioNakanishi, HiroyukiYamagishi, KoichiSagawa, YoshihikoIkeda, TakayukiMorisaki, TakayaHoashi, KojiKagisaki, HajimeIchikawa
Journal: Circulation (2014)

Background—Recently, infant cases of acute heart failure attributable to rupture of the mitral chordae tendineae have been reported. However, little is known about the pathogenesis and clinical course of this condition. Methods and Results—Ninety-five children with rupture of mitral chordae tendineae were identified in nationwide surveys of Japan diagnosed from 1995 to 2013. The clinical manifestations, management strategies, and prognosis were investigated. Eighty-one (85%) patients were between 4 and 6 months (median, 5 months) of age. In 63 (66%) patients, rupture occurred during the spring or summer. The underlying conditions before rupture included Kawasaki disease (10 cases), maternally derived anti-SSA antibodies (2 cases), and infective endocarditis (1 case). Surgery was performed in 80 patients (94 operations), and the final operations included plasty of mitral chordae in 52 cases and mechanical valve replacement in 26 cases. The histopathologic examinations of the mitral valves and chordae (n=28...