tbuckley/cabot-search huggingface dataset:
Abstract 18188: Reversible Pulmonary Trunk Banding. XI: Myocardial Vascular Endothelial Growth Factor Expression of Young Goats Submitted to Rapid Ventricle Retraining
Authors: Renato S Assad, Eduardo A Rocha, Tiago A Meniconi, Maria Cristina D Abduch, Luiz Felipe P Moreira, Vera D Aiello, Fabio B Jatene
Journal: Circulation (2016)

Introduction:  Intermittent systolic overload proved to be superior to traditional PA banding, with a more efficient ventricle hypertrophy, even with less exposure to pressure overload. There is a great interest on the adaptive mechanisms of the retrained ventricle.   Hypothesis:  This study sought to assess vascular endothelial growth factor (VEGF) expression in the subpulmonary ventricle (RV) retraining of young goats submitted to continuous versus intermittent systolic overload.   Methods:  Twenty one21 young goats were separated into 3 groups: sham (no systolic overload), continuous (continuous systolic overload), and intermittent (four 12-hour periods of systolic overload paired with a 12-hour resting period). During a 96-hour protocol, systolic overload was adjusted to achieve a 0.7 right ventricle/aortic pressure ratio. Hemodynamic evaluations were performed before and after systolic overload every day in the postoperative period. Echocardiograms were obtained in the preoperative period and at the end of protocol. After the study period, the animals were humanely killed for morphologic and vascular endothelial growth factor (VEGF) expression assessment.   Results:  Both groups had increased right ventricular and septal masses compared with the sham group (P   Conclusions:  Intermittent systolic overload promotes a greater neoangiogenesis signaling. The association of RV hypertrophy and increased VEGF expression has important implication on the ability of the heart to adapt to pressure overload by promoting a compensatory growth of the coronary vasculature, allowing for a more efficient hypertrophy.
Abstract 11205: Tropoelastin Inhibits Intimal Hyperplasia of Mouse Bioresorbable Arterial Vascular Grafts
Authors: Tadahisa Sugiura, Riddhima Agarwal, Tai Yi, Yong-Ung Lee, Shuhei Tara, Christopher K Breuer, Anthony S Weiss, Toshiharu Shinoka
Journal: Circulation (2016)

Introduction: Neointimal hyperplasia, which results from the activation, proliferation and migration of vascular smooth muscle cells (SMCs), is a detrimental condition for vascular stents or vascular grafts that leads to stenosis. Preventing neointimal hyperplasia is critically important for the success of arterial vascular grafts. Hypothesis: We assessed the hypothesis that tropoelastin seeding will prevent SMC proliferation and neointimal hyperplasia in bioresorbable arterial vascular grafts. Methods: Poly (glycolic acid) (PGA) fiber mesh coated with poly (l-lactic-co- ε -caprolactone) (PLCL) scaffolds reinforced by poly (l-lactic acid) (PLA) nano-fibers were prepared as bioresorbable arterial grafts. Tropoelastin was then seeded onto the luminal surfaces of the grafts. Female C57BL/6 mice were randomly divided into 2 groups: 1. Tropoelastin seeded group (n=10), and 2. Unseeded group (n=10). The grafts were implanted as infra-renal aortic interposition conduits. Animals were followed for 8 weeks then sacrificed to evaluate neotissue formation. Results: Tropoelastin treatment substantially reduced the number of cells that stained positive for SMC (α-SMA) and Proliferating cell nuclear antigen (PCNA) in the vessel walls (Unseeded group: 60 ± 36/HPF vs. Tropoelastin seeded group: 21 ± 13/HPF, P =0.048, Unseeded group: 11.0 ± 5.6 /HPF vs. Tropoelastin seeded group: 4.8 ± 1.7/HPF, P =0.043, SMC and PCNA, respectively, HPF; high power field) (Fig. A, B). Tropoelastin significantly reduced the thickness of the intimal layer (Unseeded group: 91 ± 22 μm vs. Tropoelastin seeded group: 50 ± 11 μm, P =0.004) (Fig. C). Conclusions: Tropoelastin seeding is beneficial in preventing SMC proliferation and neointimal hyperplasia in bioresorbable arterial vascular grafts.
Judge grants anonymity to hospital treating brain damaged baby to protect staff
Authors: Clare Dyer
Journal: BMJ (2017)

A High Court judge has granted an NHS trust caring for a seriously brain damaged baby a blanket anonymity order to prevent jigsaw identification of the baby and the sort of harassment that doctors and nurses at Great Ormond Street Hospital experienced in the Charlie Gard case.

Mr Justice Moor declared that it would be in the boy’s best interests for the hospital trust to stop providing life supporting treatment and ordered that the trust looking after him could be described only as “an NHS trust in the Midlands” in any media report.

The trust argued that staff …
Enquête européenne sur les politiques vaccinales du BCG et surveillance chez les enfants, 2005
Authors: A Infuso, D Falzon
Journal: Eurosurveillance (2006)

En 2005, les 25 pays de l’Union Europeenne (UE), ainsi qu’Andorre, la Bulgarie, la Norvege, la Roumanie et la Suisse ont participe a une enquete portant sur la vaccination par le BCG chez les enfants. Dans 12 pays, le BCG est recommande pour tous les enfants de moins de 12 mois, et dans cinq pays chez les enfants plus âges. Dix pays recommandent la vaccination chez les enfants a risque (defini par leur origine, les contacts ou les voyages), sept pays n’ont pas recours a la vaccination BCG de maniere systematique, et quatre pays pratiquent la revaccination. Dans les pays ou la vaccination par le BCG est universelle, la couverture vaccinale du BCG etait elevee (83,0% a 99,8%). Des cas de tuberculose etaient frequemment identifies chez des enfants vaccines (de 30%-98% dans cinq pays ayant une politique vaccinale universelle ou ciblee sur les groupes a haut risque). Peu d’infections dues au BCG ont ete notifiees recemment (0-1/100 000 vaccines). Les recommandations concernant le BCG sont tres variables d’un pays a l’autre, et pres de la moitie des pays participants a l’enquete prevoient des modifications, au moment ou le Centre europeen pour la prevention et le controle des maladies (ECDC) appelle a une harmonisation des strategies vaccinales. Les donnees concernant la couverture vaccinale du BCG dans les groupes cibles sont important a cet egard, mais ne sont pas souvent disponibles en Europe. Les informations sur le statut vaccinal et l’eligibilite devraient etre collectees en routine par le biais des notifications de cas de tuberculose. L’incidence des effets secondaires severes du BCG chez les enfants devrait etre etudiee. De plus, compte-tenu du manque d’evidence quant a l’efficacite de la revaccination, elle devrait etre supprimee.
Myocardial Metabolism in Endurance Exercise-Induced Left Ventricular Hypertrophy
Authors: Meagan M. Wasfy, Courtney Foster Bibbo, Marcel Brown, James R. DeLuca, Francis Wang, Brant Berkstresser, Rory B. Weiner, Gregory D. Lewis, Adolph M. Hutter, Michael H. Picard, Marcelo F. Di Carli, Aaron L. Baggish
Journal: JACC. Cardiovascular imaging (2017)
Performance of the 2017 European League Against Rheumatism/American College of Rheumatology classification criteria for adult and juvenile idiopathic inflammatory myopathies in clinical practice
Authors: Alojzija Hočevar, Ziga Rotar, Monika Krosel, Saša Čučnik, Matija Tomšič
Journal: Annals of the Rheumatic Diseases (2017)

American College of Rheumatology classification criteria for
Use of Corticosteroids to Prevent Atrial Fibrillation After Cardiac Surgery
Authors: Jaewon Oh, Ji-Hyung Chung, Seok-Min Kang
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Novel dual arginase 1/2 inhibitor OATD-02 (OAT-1746) improves the efficacy of immune checkpoint inhibitors
Authors: M.M. Grzybowski, P.S. Stańczak, J. Pęczkowicz-Szyszka, P. Wolska, A.M. Zdziarska, M. Mazurkiewicz, J. Brzezińska, R. Blaszczyk, A. Gołębiowski, P. Dobrzański, K. Dzwonek
Journal: Annals of Oncology (2017)
Abstract 49: Simultaneous quantification of HPV oncogene (E6, E7 mRNA) and PD-L1 protein expression in oral cancer samples using flow cytometry
Authors: Rian J. Morgan, Amanda N. Chargin, Haitham Mirghani, Bruce K. Patterson
Journal: Clinical Cancer Research (2017)

Abstract Background: Human papillomaviruses are a family of DNA viruses that infect the epithelium leading to the formation of lesions with the ability to progress into carcinoma in many forms. Research into causality of head and neck cancers has found a link to HPV infections affiliated with better prognosis. In addition, advances in immuno-oncology have brought immunotherapy to the forefront of cancer treatment across cancer types. Here we present data that demonstrate a combined diagnostic approach to quantify two markers important in the management of head and neck cancer. Methods: Swabs were collected from patients of Institut Gustave Roussy with lesions of the oral pharynx. Swabs were collected and placed into a collection vial with a proprietary fixation/permeabilization solution (IncellCollect, IncellDx, Inc.) and shipped overnight on cold packs for processing. Upon receipt, samples were passed through a 35 µM filter to remove aggregates. Cells were labeled with CD45 antibodies to separate epithelial from immune cells, RNA in situ hybridization with E6, E7 mRNA probes (HPV OncoTect) was performed, and these cells were labeled with PD-L1 Ab (clone 28-8) prior to analysis on the flow cytometer (CytoFlex, Beckman Coulter, Inc). Samples were also stained with DAPI to identify single nucleated cells, and to analyze cell cycle. Results: We analyzed samples from 8 patients with oral cancer with the combined E6, E7 mRNA/PD-L1 protein assay by flow cytometry. The percentage of tumor cells expressing PD-L1 averaged 9.1% in all of the samples. Of the HPV DNA positive tumor samples, the percentage of cells overexpressing HPV E6, E7 mRNA was 4.8%. Interestingly, the percentage of cells overexpressing E6, E7 mRNA that also expressed PD-L1 was statistically lower (1.4%) than the percentage of all tumor cells that expressed PD-L1 (P=0.01). Conclusions: We report a novel flow cytometric assay to quantify both HPV E6, E7 mRNA and PD-L1 simultaneously in single cells. Further, we found the expression of PD-L1 in tumor cells with HPV E6, E7 mRNA to be significantly lower than the expression of PD-L1 in tumor cells not expressing HPV E6, E7 mRNA. These findings may influence prognosis and treatment strategies. Note: This abstract was not presented at the conference. Citation Format: Rian J. Morgan, Amanda N. Chargin, Haitham Mirghani, Bruce K. Patterson. Simultaneous quantification of HPV oncogene (E6, E7 mRNA) and PD-L1 protein expression in oral cancer samples using flow cytometry [abstract]. In: Proceedings of the AACR-AHNS Head and Neck Cancer Conference: Optimizing Survival and Quality of Life through Basic, Clinical, and Translational Research; April 23-25, 2017; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2017;23(23_Suppl):Abstract nr 49.
Is There an Association Between Pre-Operative Estimated Glomerular Filtration Rates (Egfr) and Post-Operative Outcomes in Gastrointestinal Surgeries?
Authors: B. Barmayehvar, S.K. Kamarajah, M. Sowida, A. Adlan, C. Reihill, P. Ellahee
Journal: International Journal of Surgery (2017)
Tisssue factor contributes to lung metastasis and malignant pleural effusion in Stat3-activated lung adenocarcinoma
Authors: Tzy-yu Weng
Journal: Cancer Research (2007)

3087   Malignant pleural effusion (MPE) is a poor prognostic sign for patients with non-small cell lung cancer (NSCLC). The generation of MPE is largely regulated by vascular endothelial growth factor (VEGF), and upregulation of VEGF by Stat3 has been observed in several types of tumor cells. Previously, we demonstrated that autocrine IL-6 activation of Stat3 in ADCLC plays important role in the formation of malignant pleural effusion by upregulating VEGF. In this study, we established that constitutively activated Stat3 trigers tissue factor (TF) expression both in mRNA and protein level through promoter regulation in PC14PE6/AS2 human lung cancer cell line. We found that JAK kinases, but not Src kinase, are contributed in TF expression. Blockage of TF expression by siRNA in PC14PE6/AS2 decreases anchorage-independent growth in soft agar. In nude mice model, PC14P6/AS2/siTF cells produced no MPE and lung metastasis. These results suggested that TF expression plays an important role in the pathogenesis of Stat3 activated ADCLC.
Abstract 11917: Muscarinic Stimulation Facilitates SR Ca+2 Release by Increasing PKG Phosphorylation of RyR2 at Ser-2808 and Decreasing CaMKII Phosphorylation at Ser-2814
Authors: Hsiang-Ting Ho, Andriy E Belevych, Bin Liu, Ingrid M Bonilla, Przemyslaw B Radwanski, Héctor H Valdivia, Karsten Schober, Cynthia A Carnes, Sándor Györke
Journal: Circulation (2016)

Autonomic regulation of SR Ca +2  release is critical for modulating cardiac performance to match physiologic demand. During heart failure (HF), autonomic balanced shifts to a dominant sympathetic state to increase fractional SR Ca +2  release. Although intended to be compensatory, this increase in sympathetic input causes increases in the frequency of proarrhythmic spontaneous SR Ca +2  release events. Interestingly, parasympathetic augmentation was recently shown to alleviate HF phenotypes by shifting autonomic balance away from sympathetic dominance. While the effects of sympathetic stimulation and mechanism behind their action on cardiomyocyte Ca +2  handling are relatively well established in health and disease, the modes of action and mechanisms of parasympathetic modulation of SR Ca +2  release are poorly defined. To address this question, we investigated the effects of the cholinergic agonist, carbachol (CCH), on Ca +2  handling (Fluo-4 FF AM) and protein phosphorylation levels in cardiomyocytes using a combination of pharmacological approaches and genetic mouse models. In settings of reduced SR Ca +2  load, exposure to the parasympathetic agonist CCH increased fractional Ca +2  release without affecting Ca +2  spark frequency in cardiomyocytes. Immunoblot measurements showed that this “efficient” mode of excitation-contraction (EC) coupling involves reciprocal changes in the phosphorylation state of RyR2. Specifically, CCH treatment of cardiomyocytes increased Ser-2808 phosphorylation, mediated by muscarinic receptor subtype 2 and activation of PKG. Conversely, CCH treatment was associated with dephosphorylation at Ser-2814 through activation of muscarinic receptor subtype 3 and decreased ROS-dependent activation of CaMKII. We further demonstrate that parasympathetic stimulation improved Ca +2  cycling efficiency in cardiomyocytes isolated from failing hearts by restoring altered RyR2 phosphorylation balance. These results suggest that parasympathetic stimulation initiates a novel mode of EC coupling in cardiomyocytes that enhances the efficiency of cardiac SR Ca +2  store mobilization during systolic Ca +2  release, while inhibiting pathologic diastolic Ca +2  leak.
Scleroderma Peripheral B Lymphocytes Secrete Interleukin‐6 and Transforming Growth Factor β and Activate Fibroblasts
Authors: Nicolas Dumoitier, Benjamin Chaigne, Alexis Régent, Sébastien Lofek, Maissa Mhibik, Peter Dorfmüller, Benjamin Terrier, Jonathan London, Alice Bérezné, Nicolas Tamas, Nadine Varin‐Blank, Luc Mouthon
Journal: Arthritis & Rheumatology (2016)

To study the role of B lymphocytes in systemic sclerosis (SSc).Peripheral B cell subpopulations and the production of interleukin-6 (IL-6) and transforming growth factor β (TGFβ) were analyzed using flow cytometry and multiplex assay. The fibroblast proliferation rate upon incubation with supernatants from B cells isolated from SSc patients or healthy controls was assessed using XTT, bromodeoxyuridine, and Ki-67. Collagen production was assessed using a collagen assay.Ninety untreated patients (12 males) fulfilling the American College of Rheumatology/European League Against Rheumatism criteria for SSc (23 with diffuse cutaneous SSc [dcSSc] and 67 with limited cutaneous SSc [lcSSc]) and 30 healthy controls were recruited. Increased proportions of B cells expressing CD69 and CD95 were identified among the patients with SSc. B lymphocytes from dcSSc patients versus lcSSc patients and healthy controls expressed increased proportion of cells positive for CD5 (mean ± SD 24.12 ± 7.93% versus 14.09 ± 6.58% [P = 0.03] and 14.21 ± 5.34% [P = 0.01]), CD86 (39.89 ± 22.11% versus 17.72 ± 13.98% [P = 0.0007] and 11.68 ± 11.09% [P < 0.001]), IL-6 receptor (IL-6R; 33.64 ± 23.12% versus 17.91 ± 13.62% [P < 0.0001] and 12.08 ± 8.68% [P = 0.0009]), or IL-21R (32.55 ± 20.19% versus 5.76 ± 4.40% [P < 0.0001] and 5.93 ± 3.29% [P < 0.0001]). In addition, the levels of IL-6 (mean ± SD 314.3 ± 317.8 pg/ml versus 6.10 ± 2.58 pg/ml; P = 0.0007) and TGFβ (mean ± SD 1,020 ± 569 pg/ml versus 163.8 ± 98.69 pg/ml; P = 0.001) secreted by B lymphocytes from patients with SSc were increased compared to healthy controls. Fibroblast proliferation and collagen production were also significantly increased in the presence of B cell supernatant from SSc patients as compared to healthy controls.The numbers of activated B cells were increased in SSc patients, and the up-regulation of CD5, CD86, IL-6R, and IL-21R discriminated between patients with dcSSc and those with lcSSc. Peripheral B lymphocytes from SSc patients secreted both IL-6 and TGFβ, and they activated fibroblasts in vitro.
Targeting of EWS/FLi-1 by siRNA inhibits survivin expression and sensitizes ESFT cells to TRAIL
Authors: Guang Li, Maria Tsokos
Journal: Cancer Research (2007)

522   Ewing′s sarcoma family of tumors (ESFT) are characterized by the presence of the EWS/FLi-1 chimeric transcript which mediates survival of ESFT cells. Current treatment is curative only in half of ESFT patients. Treatment failures are due to persistence of drug-resistant disseminated tumor cells. It has been postulated that these cells do not proliferate and temporarily fail to express the EWS/FLi-1 transcript. In order to simulate the in vivo dormant cell status, we created stable EWS/FLi-1-silenced clones by transfecting EWS/FLi-1 fusion-specific siRNA into the TC71 ESFT cells. All clones showed significant G1/ G0 cell cycle arrest and became highly sensitive to TRAIL, in contrast to the parent TC71 cells. To investigate why TRAIL resistance was reversed in these clones, we searched for expression of antiapoptotic (survivin, bcl2, FLIP etc.) and proapoptic (caspases 8 and 3, TRAIL receptors etc.) proteins by Western blotting. We found that only survivin expression was decreased significantly in the EWS/FLi-1-silenced cells, suggesting an EWS/FLi-1 regulatory role in survivin expression. Luciferase reporter assays in both cycling and mimosine G1/ G0 arrested embryonic kidney cells (HEK293) showed that wild type EWS/FLi-1 induced 10~20 folds survivin promoter activity in a cell cycle independent manner, in contrast to DNA-binding deficient EWS/FLi-1 mutants, which did not. Also, reduced survivin promoter activity was observed in luciferase reporter assays of transient EWS/FLi-1 siRNA TC71 transfectants. Chromatin immunoprecipitation assays showed no direct binding of EWS/FLi-1 to survivin promoter. Therefore, we hypothesized the involvement of an intermediatiary pathway, such as NF-kB, which has been implicated in the regulation of antiapoptotic proteins including survivin. Luciferase reporter assays in parent TC71 cells and in EWS/FLi-1-transfected HEK293 cells in the presence or absence of the NF-kB inhibitor BAY11-7082 showed that BAY11-7082 blocked efficiently EWS/FLi-1-induced survivin promoter activity. Our results indicate that downregulation of EWS/FLi-1 results in transcriptional downregulation of survivin, possibly through NF-kB and reverses TRAIL resistance. Therefore, ESFT patients with dormant drug-resistant cells, which do not express EWS/FLi-1, may benefit from TRAIL treatment.
Impact of prediagnostic smoking and smoking cessation on colorectal cancer prognosis: a meta-analysis of individual patient data from cohorts within the CHANCES consortium
Authors: J.M. Ordóñez-Mena, V. Walter, B. Schöttker, M. Jenab, M.G. O’Doherty, F. Kee, B. Bueno-de-Mesquita, P.H.M. Peeters, B.H. Stricker, R. Ruiter, A. Hofman, S. Söderberg, P. Jousilahti, K. Kuulasmaa, N.D. Freedman, T. Wilsgaard, A. Wolk, L.M. Nilsson, A. Tjønneland, J.R. Quirós, F J B van Duijnhoven, P.D. Siersema, P. Boffetta, A. Trichopoulou, H. Brenner
Journal: Annals of Oncology (2017)
Abstract 13690: Longitudinal and Segmental Right Ventricular Free-Wall Strain Before and After Pulmonary Thromboendarterectomy in Patients With Chronic Thromboembolic Pulmonary Hypertension
Authors: Darrin Wong, Omid Yousefian, William Auger, Teri Dittrich, Michael Madani, Lori Daniels, Ajit Raisinghani, Anthony DeMaria, Daniel Blanchard
Journal: Circulation (2015)

Introduction: Echocardiographic assessment of right ventricular (RV) function is problematic. We sought to evaluate RV systolic function in chronic thromboembolic pulmonary hypertension (CTEPH) using 2D speckle tracking and strain analysis. We assessed longitudinal &amp; segmental RV free-wall strain before and after pulmonary thromboendarterectomy (PTE) in patients with CTEPH. Hypothesis: We hypothesized systolic strain would improve after PTE. Methods: Peak systolic strain (%) of the RV free-wall and its segments (basal, mid, apical) was measured by 2D echocardiography in 127 consecutive CTEPH patients (52 ± 14 years, 49% female) with adequate images pre- &amp; post- PTE. Strain was measured 9.7 ± 17.3 days pre- and 11.3 ± 17.0 days post-PTE using Epsilon Imaging EchoInsight software. Results: Mean longitudinal strain of the RV remained the same pre-PTE compared to post-PTE (-11.4 ± 5.7 to 11.2 ± 5.6%, p = 0.67), but basal &amp; mid RV free-wall strain worsened (less negative): -17.1% to -14.1% (p=0.003) and -12.8% to -10% (p=0.001) respectively after PTE. Apical RV free-wall strain improved (more negative): -3.7% to -9.2% (p&lt;0.001) (Table 1). Pre-PTE, mean RV free-wall strain correlated significantly with pulmonary vascular resistance (PVR) (Table 2) and mean pulmonary artery pressure (mPAP), but this relationship disappeared after PTE. Conclusions: RV free wall basal and mid segment systolic strain worsened (less negative) soon after PTE, while the apical segment strain improved (more negative). The reasons for these differences are unclear, but may vary due to RV stunning and changes in LV filling. RV mean free-wall strain correlates significantly with PVR pre- but not post-PTE.
Policy makers ignore the role of evidence based treatment for opioid dependence
Authors: Ian Hamilton
Journal: BMJ (2017)

Makary and colleagues, refreshingly, acknowledge that doctors’ habits have contributed to the inappropriate introduction and management of opiates.1 I have some cautionary points to add. We must be careful that, in raising awareness of this matter, people who have a clear medical need for these drugs are not denied them; an over-reaction would …