tbuckley/cabot-search huggingface dataset:
Radiation enhanced MMP-9 dependent migration/invasion in hepatocellular carcinoma cell through PI3K/Akt/NF-kB or MAPK/ERK/NF-kB pathways
Authors: Jodi Johnson, Yuangang Liu, Sarah Lawson, Molly Kulesz-Martin
Journal: Cancer Research (2005)

5691   A view of all three members of the p53 family (p53, p63, p73) is needed to understand how they may cooperate to carry out activities in tumor suppression and response to ionizing (IR) and ultraviolet B (UVB) radiation. We investigated the roles in cell cycle arrest and apoptosis response to DNA damage of the endogenous p53 family member proteins in genetically related initiated control (03C) and malignant (03R) mouse keratinocytes. The malignant 03R cells possess a wt p53 gene but defective p53 protein activities, reduced overall expression of p63 protein, and a striking loss of p73 protein. The DNA affinity immunoblotting assay [Liu et al., 2001; Knights et al., 2003] detected endogenous p53 family member binding in vitro to target DNA sequences of p21 (cell cycle arrest) and Noxa (apoptosis). p53 family member proteins from initiated 03C cells treated with either IR or UVB light bound strongly to the p21 and Noxa DNA probes. This binding correlated with an increase in p21 and Noxa mRNA (as analyzed by quantitative real-time PCR), and increased cell cycle arrest and apoptosis compared to the untreated control. In contrast, the IR treated malignant 03R cells exhibited reduced p53 family member DNA binding to p21 and Noxa probes, a lack of p21 or Noxa mRNA induction, failure to arrest in G1, and a lack of apoptotic response. Surprisingly, the IR-resistant 03R cells were sensitized to UVB-induced apoptosis. The UVB-induced apoptosis in 03R cancer cells and the apoptosis response of 03C initiated cells correlated with an increased binding to Noxa of p53 and p63 isoforms (putative TAp63 alpha and beta), and upregulation of Noxa mRNA expression. Furthermore, following DNA damage, increased p63 binding to the Noxa binding site coincided with decreased p73 isoforms binding to Noxa. We will selectively modulate expression of p53 family member isoforms to explore the idea that specific isoforms of p63 and p73 coordinate with p53 in binding to cell cycle and apoptosis-related promoters to determine cell fate and may restore cell cycle arrest and apoptotic response in malignant cells. Insight into p53 family members’ roles in the etiology of radiation response in epithelial cancer development could suggest new therapeutic approaches to IR radiation-resistant human solid tumors of epithelia. Supported by NCI CA31101, CA98893, OHSU Cancer Institute CA69533, Medical Research Foundation of Oregon, the Tartar Trust Fellowship, and the Department of Dermatology, OHSU.
The Clinical Features and Underlying Pathophysiology of Myocardial Infarction with No Obstructive Coronary Atherosclerosis: The Role of Optical Coherence Tomography
Authors: Takahashi Tatsunori, Okayama Hideki, Hosokawa Saki, Kosaki Tetsuya, Nakagawa Hirohiko, Kawamura Go, Shigematsu Tatsuya, Kinoshita Masaki, Kawada Yoshitaka, Hiasa Go, Yamada Tadakatsu, Kazatani Yukio
Journal: Circulation (2016)
Abstract P087: Hydralazine and Nitroglycerin Combination Improves Excitation-Contraction Coupling in Failing Cardiomyocytes
Authors: Raul A Dulce, Daniel R Gonzalez, Joshua M Hare
Journal: Circulation Research (2011)

Although hydralazine and organic nitrates have proven clinical benefits for heart failure, the underlying mechanism of action of this combination remains obscure. Spontaneously Hypertensive Heart Failure (SHHF) rats are a representative heart failure model which shares common phenotypic features with human failing hearts. We have recently shown that SHHF cardiomyocytes exhibit depressed contractility and calcium transient amplitude in response to increasing rate and increased sarcoplasmic reticulum (SR) calcium leak, typical characteristics observed in heart failure. The purpose of this study was to test the hypothesis that hydralazine, alone or in combination with nitroglycerin, restores calcium cycling and contractile performance in cardiomyocytes from SHHF rats. We measured sarcomere length (SL) shortening and calcium transient amplitude (Δ[Ca 2+ ] i ) in isolated cardiac myocytes from male SHHF rats (22–24 months old, n=4) and their normotensive controls Wistar-Kyoto rats (18–22 months old, n=4) during pacing (0.5–4 Hz). Both SL shortening and Δ[Ca 2+ ] i responses to increasing pacing in SHHF cardiomyocytes were augmented by 10 μM hydralazine. The same concentration of nitroglycerin alone did not significantly affect these parameters. In response to the combination of these two drugs, there was a strong trend toward improved contractility and normal Δ[Ca 2+ ] i . We also assessed SR Ca 2+ leak by a brief incubation with tetracaine following by a caffeine challenge, a regimen designed to test SR Ca 2+ load. At matched SR Ca 2+ content, Ca 2+ leak was not significantly changed by nitroglycerin or hydralazine alone. However, in combination they restored the leak in SHHF myocytes to the normal levels seen in WKY myocytes. Hydralazine improves cardiac excitation-contraction coupling that is impaired in this model, whereas nitroglycerin does not exert a significant effect on this response. However, when administered together, nitroglycerin appears to modulate the enhancing effect induced by hydralazine and helps to restore normal SR calcium leakage. These data offer new insights into the mechanisms underlying the actions of hydralazine and organic nitrates on heart failure.
Effect of HSV-2 infection on subsequent HIV acquisition: an updated systematic review and meta-analysis
Authors: Katharine J Looker, Jocelyn A R Elmes, Sami L Gottlieb, Joshua T Schiffer, Peter Vickerman, Katherine M E Turner, Marie-Claude Boily
Journal: The Lancet Infectious Diseases (2017)

HIV and herpes simplex virus type 2 (HSV-2) infections cause a substantial global disease burden and are epidemiologically correlated. Two previous systematic reviews of the association between HSV-2 and HIV found evidence that HSV-2 infection increases the risk of HIV acquisition, but these reviews are now more than a decade old.For this systematic review and meta-analysis, we searched PubMed, MEDLINE, and Embase (from Jan 1, 2003, to May 25, 2017) to identify studies investigating the risk of HIV acquisition after exposure to HSV-2 infection, either at baseline (prevalent HSV-2 infection) or during follow-up (incident HSV-2 infection). Studies were included if they were a cohort study, controlled trial, or case-control study (including case-control studies nested within a cohort study or clinical trial); if they assessed the effect of pre-existing HSV-2 infection on HIV acquisition; and if they determined the HSV-2 infection status of study participants with a type-specific assay. We calculated pooled random-effect estimates of the association between prevalent or incident HSV-2 infection and HIV seroconversion. We also extended previous investigations through detailed meta-regression and subgroup analyses. In particular, we investigated the effect of sex and risk group (general population vs higher-risk populations) on the relative risk (RR) of HIV acquisition after prevalent or incident HSV-2 infection. Higher-risk populations included female sex workers and their clients, men who have sex with men, serodiscordant couples, and attendees of sexually transmitted infection clinics.We identified 57 longitudinal studies exploring the association between HSV-2 and HIV. HIV acquisition was almost tripled in the presence of prevalent HSV-2 infection among general populations (adjusted RR 2·7, 95% CI 2·2-3·4; number of estimates [Ne]=22) and was roughly doubled among higher-risk populations (1·7, 1·4-2·1; Ne=25). Incident HSV-2 infection in general populations was associated with the highest risk of acquisition of HIV (4·7, 2·2-10·1; Ne=6). Adjustment for confounders at the study level was often incomplete but did not significantly affect the results. We found moderate heterogeneity across study estimates, which was explained by risk group, world region, and HSV-2 exposure type (prevalent vs incident).We found evidence that HSV-2 infection increases the risk of HIV acquisition. This finding has important implications for management of individuals diagnosed with HSV-2 infection, particularly for those who are newly infected. Interventions targeting HSV-2, such as new HSV vaccines, have the potential for additional benefit against HIV, which could be particularly powerful in regions with a high incidence of co-infection.World Health Organization.
Recombinant Influenza Vaccine More Effective in Older Adults
Authors: Anita Slomski
Journal: JAMA (2017)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Five-Year Outcomes after On-Pump and Off-Pump Coronary-Artery Bypass
Authors: A. Laurie Shroyer, Brack Hattler, Todd H. Wagner, Joseph F. Collins, Janet H. Baltz, Jacquelyn A. Quin, G. Hossein Almassi, Elizabeth Kozora, Faisal Bakaeen, Joseph C. Cleveland, Muath Bishawi, Frederick L. Grover
Journal: New England Journal of Medicine (2017)

Coronary-artery bypass grafting (CABG) surgery may be performed either with cardiopulmonary bypass (on pump) or without cardiopulmonary bypass (off pump). We report the 5-year clinical outcomes in patients who had been included in the Veterans Affairs trial of on-pump versus off-pump CABG.From February 2002 through June 2007, we randomly assigned 2203 patients at 18 medical centers to undergo either on-pump or off-pump CABG, with 1-year assessments completed by May 2008. The two primary 5-year outcomes were death from any cause and a composite outcome of major adverse cardiovascular events, defined as death from any cause, repeat revascularization (CABG or percutaneous coronary intervention), or nonfatal myocardial infarction. Secondary 5-year outcomes included death from cardiac causes, repeat revascularization, and nonfatal myocardial infarction. Primary outcomes were assessed at a P value of 0.05 or less, and secondary outcomes at a P value of 0.01 or less.The rate of death at 5 years was 15.2% in the off-pump group versus 11.9% in the on-pump group (relative risk, 1.28; 95% confidence interval [CI], 1.03 to 1.58; P=0.02). The rate of major adverse cardiovascular events at 5 years was 31.0% in the off-pump group versus 27.1% in the on-pump group (relative risk, 1.14; 95% CI, 1.00 to 1.30; P=0.046). For the 5-year secondary outcomes, no significant differences were observed: for nonfatal myocardial infarction, the rate was 12.1% in the off-pump group and 9.6% in the on-pump group (P=0.05); for death from cardiac causes, the rate was 6.3% and 5.3%, respectively (P=0.29); for repeat revascularization, the rate was 13.1% and 11.9%, respectively (P=0.39); and for repeat CABG, the rate was 1.4% and 0.5%, respectively (P=0.02).In this randomized trial, off-pump CABG led to lower rates of 5-year survival and event-free survival than on-pump CABG. (Funded by the Department of Veterans Affairs Office of Research and Development Cooperative Studies Program and others; ROOBY-FS ClinicalTrials.gov number, NCT01924442 .).
癌の三重様式イメージングと熱変調された光線力学的治療のための活性化可能なアルブミン光増感剤ナノ集合体【Powered by NICT】
Authors: Hu Dehong, Sheng Zonghai, Gao Guanhui, Siu Fungming, Liu Chengbo, Wan Qian, Gong Ping, Zheng Hairong, Ma Yifan, Cai Lintao
Journal: Biomaterials (2016)
Note on the Copper Content of the Tissues of Tumor-Bearing Animals
Authors: Jesse P. Greenstein, J. W. Thompson
Journal: JNCI Journal of the National Cancer Institute (1943)

Journal Article Note on the Copper Content of the Tissues of Tumor-Bearing Animals Get access Jesse P. Greenstein, Jesse P. Greenstein senior biochemist Search for other works by this author on: Oxford Academic PubMed Google Scholar J. W. Thompson J. W. Thompson pharmacologist National Cancer Institute, National Institute of Health, United States Public Health Service Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 3, Issue 4, February 1943, Pages 405–408, https://doi.org/10.1093/jnci/3.4.405 Published: 01 February 1943
Post-discharge, Multidisciplinary Comprehensive Intervention in Cardiac Rehabilitation Ameliorates Prognostic Risk and Depression in Patients after Cardiac Events in Modern Era
Authors: Arakawa Tetsuo, Kumasaka Reon, Nakanishi Michio, Nakao Kazuhiro, Fukui Shigefumi, Ohhara Takahiro, Yanase Masanobu, Noguchi Teruo, Yasuda Satoshi, Goto Yoichi
Journal: Circulation (2016)
ナノ粒子促進オートファジー阻害は乳癌幹細胞に対する化学療法剤の有効性を促進する【Powered by NICT】
Authors: Sun Rong, Shen Song, Zhang Yun-Jiao, Xu Cong-Fei, Cao Zhi-Ting, Wen Long-Ping, Wang Jun
Journal: Biomaterials (2016)
<i>G</i> but not <i>g</i>: In search of the evolutionary continuity of intelligence
Authors: Moran Bar-Hen-Schweiger, Avraham Schweiger, Avishai Henik
Journal: Behavioral and Brain Sciences (2017)

Abstract Conceptualizing intelligence in its biological context, as the expression of manifold adaptations, compels a rethinking of measuring this characteristic in humans, relying also on animal studies of analogous skills. Mental manipulation , as an extension of object manipulation, provides a continuous, biologically based concept for studying G as it pertains to individual differences in humans and other species.
Exploitation of Gene Expression and Cancer Biomarkers in Paving the Path to Era of Personalized Medicine
Authors: Hala Fawzy Mohamed Kamel, Hiba Saeed A. Bagader Al-Amodi
Journal: Genomics Proteomics & Bioinformatics (2017)

Cancer therapy agents have been used extensively as cytotoxic drugs against tissue or organ of a specific type of cancer. With the better understanding of molecular mechanisms underlying carcinogenesis and cellular events during cancer progression and metastasis, it is now possible to use targeted therapy for these molecular events. Targeted therapy is able to identify cancer patients with dissimilar genetic defects at cellular level for the same cancer type and consequently requires individualized approach for treatment. Cancer therapy begins to shift steadily from the traditional approach of "one regimen for all patients" to a more individualized approach, through which each patient will be treated specifically according to their specific genetic defects. Personalized medicine accordingly requires identification of indicators or markers that guide in the decision making of such therapy to the chosen patients for more effective therapy. Cancer biomarkers are frequently used in clinical practice for diagnosis and prognosis, as well as identification of responsive patients and prediction of treatment response of cancer patient. The rapid breakthrough and development of microarray and sequencing technologies is probably the main tool for paving the way toward "individualized biomarker-driven cancer therapy" or "personalized medicine". In this review, we aim to provide an updated knowledge and overview of the current landscape of cancer biomarkers and their role in personalized medicine, emphasizing the impact of genomics on the implementation of new potential targeted therapies and development of novel cancer biomarkers in improving the outcome of cancer therapy.
Abstract 179: EGFR Transactivation Triggered by Alpha1a-Adrenergic Receptor Genetic Variant is Generalizable Phenomenon in Cardiovascular Cells
Authors: Anush Oganesian, Irina Gradinaru, Ekaterina Babaeva, Debra A Schwinn
Journal: Circulation Research (2013)

Alpha 1 -Adrenergic Receptors (α 1 AR) are G protein-coupled transmembrane receptors (GPCRs) that mediate actions of the sympathetic nervous system through binding of endogenous catecholamines epinephrine and norepinephrine. Three subtypes of α 1 ARs (α 1a , α 1b , α 1d ) are expressed within most sympathetic tissues and in the human heart and they couple predominantly to the G q/11 family of G proteins. α 1a ARs play a major role in regulating vascular tone, in blood vessel repair and in cardiovascular diseases like hypertension, myocardial hypertrophy, ischemia and heart failure. Recently, we discovered a novel, unique mechanism of hypertension triggered by naturally occurring human α 1a AR-G247R (247R) genetic variant, identified in the 3 rd intracellular loop (3iL) of the receptor in hypertensive patient. 247R signals in Gq-independent but βarrestin1-dependent manner leading to transactivation of epidermal growth factor receptor (EGFR) via constitutive active coupling to the βarrestin1 /MMP7/EGFR pathway. We also demonstrated that expression of 247R in cardiomyoblasts leads to phenotypical and morphological changes of the cells to novel fibroblast-like phenotype. Here we examine if the 247R-triggered hyperproliferation is generalizable in other cell types such as smooth muscle cells (SMC). Our preliminary results reveal that constitutive expression of 247R in human coronary artery SMCs and in A-10 rat cell line leads to ~2-fold increased hyperproliferation and ERK-kinase activation. The increased proliferation of 247R cells is reduced by EGFR specific inhibitor AG1478 and general MMP inhibitor GM6001, indicating involvement of EGFR transactivation pathway in 247R-triggered hyperproliferation. We elucidate molecular mechanisms and signal transduction pathways responsible for increased cell proliferation and hypertrophy in SMCs. Search of SNP databases revealed several additional SNPs in the same 3iL of human α 1a AR. The structure modeling reveals that 247R is a representative of these SNPs, and novel EGFR transactivation pathway triggered by 247R may represent general mechanism for SNPs identified in the 3iL of α 1a AR involved in development of hypertension.
Abstract P226: Improved Insulin Sensitivity with a Healthy Low Fat or a Healthy Low Carbohydrate Weight Loss Diet: A Twelve Month Randomized Trial
Authors: John F Trepanowski, Christopher D Gardner
Journal: Circulation (2017)

Background: The abilities of healthy low fat (HLF) and healthy low carbohydrate (HLC) weight loss diets to improve insulin sensitivity over the long term are not well understood. Objective: To examine whether HLF and HLC weight loss diets improve insulin sensitivity over 12 months, and to determine if either diet is superior for these improvements. Methods: Healthy adults without diabetes, aged 18-50 years, BMI between 28 and 40 kg/m 2 , were randomized to HLF or HLC for 12 months (n = 609). Health educators delivered the interventions in 22 1-hr group classes. Dietary intake was assessed by three 24-hour recalls per time point. Glucose and insulin were measured at times 0, 30, 60, and 120 min during a 75-g oral glucose tolerance test. Indices of hepatic insulin sensitivity (homeostasis model assessment of insulin resistance; HOMA) and whole-body insulin sensitivity (Matsuda index and the plasma concentration ratio of triglyceride to HDL-cholesterol; TG/HDL-C) were measured. Data were collected at baseline and 12 months. Statistical analyses were performed on study completers. Results: Complete data were available for n = 193 for HLF, and n = 197 for HLC. BMI and all indices of insulin sensitivity were similar between groups at baseline (p &gt; 0.25 for all). Macronutrient distributions at 12 months were 48% vs. 30% carbohydrate, 29% vs. 45% fat, and 21% vs. 23% protein for HLF and HLC, respectively. Changes in BMI (mean ± SEM) were similar between groups (HLF: 33.38 ± 0.28 to 31.48 ± 0.29; HLC: 33.01 ± 0.25 to 30.69 ± 0.28; p = 0.11). Improvements within each group were observed for HOMA (HLF: 3.81 ± 0.17 to 3.16 ± 0.12; HLC: 3.60 ± 0.13 to 3.00 ± 0.12), Matsuda index (HLF: 3.13 ± 0.14 to 3.92 ± 0.18; HLC: 3.10 ± 0.12 to 3.86 ± 0.14), and TG/HDL-C (HLF: 3.16 ± 0.17 to 2.74 ± 0.14; HLC: 2.91 ± 0.15 to 2.09 ± 0.14) (p &lt; 0.001 for all). The improvement in TG/HDL-C was greater for HLC (p = 0.013), while the improvements in HOMA and Matsuda index did not differ significantly between groups (p &gt; 0.75 for both). Conclusions: All indices of insulin sensitivity were improved with both diets after 12 months. Neither diet was superior for improving HOMA. The HLC diet was more effective for improving TG/HDL-C, but not the Matsuda index.
Clinical correlates of decreased anteroposterior metabolic gradients in positron emission tomography (PET) of schizophrenic patients
Authors: L. E. DeLisi, M. S. Buchsbaum, H. H. Holcomb, S. Dowling-Zimmerman, D. Pickar, J. Boronow, J. M. Morihisa, D. P. van Kammen, W. Carpenter, R. Kessler
Journal: American Journal of Psychiatry (1985)

The finding in schizophrenic patients of a reversal of the normal frontal to posterior pattern of brain metabolic activity with positron emission tomography (PET) is of interest, but its relevance to psychopathology is unknown. Using PET, the authors studied 21 patients with chronic schizophrenia and 21 age- and sex-matched control subjects. Although eight of the 21 patients and only one of the control subjects showed a relatively lower anteroposterior metabolic gradient, no clinical correlates of this finding were noted. In addition, cerebral atrophy, as determined by CAT scan, was not associated with this aberrant metabolic pattern.
Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions
Authors: Ana Martín-Pardillos, Anastasia Tsaalbi-Shtylik, Si Chen, Seka Lazare, Ronald P. van Os, Albertina Dethmers-Ausema, Nima Borhan Fakouri, Matthias Bosshard, Rossana Aprigliano, Barbara van Loon, Daniela C. F. Salvatori, Keiji Hashimoto, Celia Dingemanse-van der Spek, Masaaki Moriya, Lene Juel Rasmussen, Gerald de Haan, Marc H. G. P. Raaijmakers, Niels de Wind
Journal: Blood (2017)
Disentangling <i>learning</i> from <i>knowing</i>: Does associative learning ability underlie performances on cognitive test batteries?
Authors: Jayden O. van Horik, Stephen E. G. Lea
Journal: Behavioral and Brain Sciences (2017)

Abstract Are the mechanisms underlying variations in the performance of animals on cognitive test batteries analogous to those of humans? Differences might result from procedural inconsistencies in test battery design, but also from differences in how animals and humans solve cognitive problems. We suggest differentiating associative-based ( learning ) from rule-based ( knowing ) tasks to further our understanding of cognitive evolution across species.
Age up to 75 Years Does Not Influence the Outcomes of Allogeneic Hematopoietic Stem Cell Transplantation in Acute Myeloid Leukemia and Myelodysplastic Syndrome
Authors: Dipenkumar Modi, Abhinav Deol, Seongho Kim, Kendra Mellert, Marie Ventimiglia, Alavi Asif, Divaya Bhutani, Lois Ayash, Voravit Ratanatharathorn, Joseph P. Uberti
Journal: Blood (2016)