tbuckley/cabot-search huggingface dataset:
Abstract 2832: Novel therapy for oral squamous cell carcinoma using non-thermal atmospheric pressure biocompatible plasma activated water
Authors: Ihn Han, In Seok Song, Ying Li, Min Ho Kang, Jae Jun Ryu, Eun Ha Choi
Journal: Cancer Research (2018)

Abstract Non-thermal atmospheric pressure biocompatible plasma (NBP) has been reported that was possible to induce apoptosis on various cancer cells including lung, ovary, brain, liver, breast, and pancreas cancer. We developed NBP device, which has been reported to have cytotoxicity on cancer cells induce DNA damage and apoptosis. In particular,reactive oxygen and/or nitrogen species (RONS) were generated by plasma device to give oxidative stress and depolarization of mitochondria membrane in cancer cells. Now we developed a microwave plasma jet system, which could generate nitric oxide (NO) and NO-plasma activated water (NO-PAW). Previously, we found that NO-PAW could induce apoptosis and accumulate ROS in human cervical cancer cells (1). In this study we investigated the efficacy of NO-PAW on oral squamous cancer cells (OSCC), a common malignant tumor of the head and neck (2, 3). We observed that cytotoxicity and apoptotic stimulation by NO-PAW were significantly increased. Also, expression level of caspase and PARP in OSCC was increased after treatment by NO-PAW. However, the apoptosis effect was not observed in human gingival fibroblasts (as normal human cells). Additionally, we found that NO-PAW inhibited cancer progression in vivo. In conclusion, NO-PAW could suppress the progress of OSCC by inducing cell apoptosis. This indicated NO-PAW as a novel tool for OSCC therapy, and probably will be also effective for other oral cancer. Acknowledgments: This work was supported by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF- 2016K1A4A3914113) funded by the Ministry of Science, ICT, and Future Planning (MSIP) of the Korean Government for EH. Choi, I. Han. The Korea government, and the Basic Science Research Program through the NRF of Korea, funded by the Ministry of Education (NRF-2015R1C1A2A01054137) for I. Han. References: 1. Y. Li, M.H. Kang, H.S. Uhm, G.J. Lee, E.H. Choi, I. Han. Sci Rep 2017;7:1. 2. E. Majchrzak, B. Szybiak, A. Wegner, P. Pienkowski, J. Pazdrowski, L. Luczewski, M. Sowka, P. Golusinski, J. Malicki, W. Golusinski. Radiol Oncol 2014;48:1. 3. B. Wang, S. Zhang, K. Yue, X.D. Wang. Chin J Cancer 2013;32:614. Citation Format: Ihn Han, In Seok Song, Ying Li, Min Ho Kang, Jae Jun Ryu, Eun Ha Choi. Novel therapy for oral squamous cell carcinoma using non-thermal atmospheric pressure biocompatible plasma activated water [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 2832.
Mammalian Target of Rapamycin Signaling in Cardiac Physiology and Disease
Authors: SebastianoSciarretta, MassimoVolpe, JunichiSadoshima
Journal: Circulation Research (2014)

The protein kinase mammalian or mechanistic target of rapamycin (mTOR) is an atypical serine/threonine kinase that exerts its main cellular functions by interacting with specific adaptor proteins to form 2 different multiprotein complexes, mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). mTORC1 regulates protein synthesis, cell growth and proliferation, autophagy, cell metabolism, and stress responses, whereas mTORC2 seems to regulate cell survival and polarity. The mTOR pathway plays a key regulatory function in cardiovascular physiology and pathology. However, the majority of information available about mTOR function in the cardiovascular system is related to the role of mTORC1 in the unstressed and stressed heart. mTORC1 is required for embryonic cardiovascular development and for postnatal maintenance of cardiac structure and function. In addition, mTORC1 is necessary for cardiac adaptation to pressure overload and development of compensatory hypertrophy. However, partial and selective pharmacolog...
Serotonin Increases Interleukin-6 Synthesis in Human Vascular Smooth Muscle Cells
Authors: TakayukiIto, UichiIkeda, MasahisaShimpo, KeijiYamamoto, KazuyukiShimada
Journal: Circulation (2000)

Background—Interleukin-6 (IL-6) is a key molecule in chronic inflammation and has been implicated in the progression of atherosclerosis. Serotonin (5-hydroxytryptamine; 5-HT) causes vascular contraction and proliferation, but its role in atherogenesis has not been clarified. We investigated the effects of 5-HT on IL-6 synthesis in human vascular smooth muscle cells (VSMCs). Methods and Results—IL-6 levels in the culture medium of VSMCs were determined by ELISA. IL-6 mRNA accumulation was determined by use of a Quantikine mRNA colorimetric quantification kit. NF-κB activation was tested by gel retardation assay. 5-HT induced IL-6 production by VSMCs in a time- and dose-dependent manner, with increased IL-6 mRNA accumulation and nuclear factor-κB activation. The effect of 5-HT on IL-6 production was significantly inhibited by the 5-HT2 receptor antagonist ketanserin and the selective 5-HT2A receptor antagonist sarpogrelate. Conversely, the 5-HT2 receptor agonist α-methyl-5-HT increased IL-6 production. The ...
Macromolecular Composition of Stress Fiber–Plasma Membrane Attachment Sites in Endothelial Cells In Situ
Authors: YumikoKano, KazuoKatoh, MichitakaMasuda, KeigiFujiwara
Journal: Circulation Research (1996)

Stress fibers (SFs) are present along the apical (apical SF) and the basal (basal SF) portions of cultured cells. We have recently shown that apical SFs are anchored to the apical plasma membrane (PM) in a manner similar to how basal SFs are attached to focal adhesion sites. We propose calling such apical SF–membrane attachment sites “apical plaques.” To study the macromolecular composition of the apical plaque and the focal adhesion in endothelial cells (ECs) in situ, we examined by confocal laser scanning and fluorescence microscopy guinea pig aortae stained with various antibodies against focal adhesion–associated proteins. Basal SFs oriented parallel to the blood flow direction were mainly located in the upstream half of the cell. Thin apical SFs were also observed. Spotty staining patterns were observed in the basal and the apical portions of cells stained with anti-vinculin, anti-talin, anti-paxillin, or anti-fibronectin receptor, indicating the presence of focal adhesions and apical plaques in ECs ...
Structural Basis for the Static Mechanical Properties of the Aortic Media
Authors: Harveywolinsky, Seymourglagov
Journal: Circulation Research (1964)

The arrangement and interrelation of the structural components of the rabbit aorta were studied by light and electron microscopy. Segments of abdominal aorta were restored to in vivo length and fixed in formalin or osmic acid while intraluminal pressures ranging from 0 to 200 mm Hg were maintained by a constant pressure perfusion apparatus. Transverse, longitudinal, and tangential sections of vessels fixed at various distending pressures were examined. Micrometric measurements included vessel diameters and wall thickness, thickness and waviness of elastin lamellae and interlamellar distances. With increasing pressures below the diastolic value, aortic radius increased and wall thickness decreased rapidly. Waviness of the tubular elastin lamellae decreased uniformly throughout the wall. Interlamellar distances decreased uniformly and markedly. Lamellar thicknesses decreased uniformly but much less than interlamellar distances. A fine fibrillary elastin network connected the thick lamellae. Collagen fibers ...
Abstract LB-329: Multiple-resolution characterization of tumor heterogeneity as associated with disease progression in a mouse model of colorectal cancer
Authors: Mohammed Farhoud, Alyssa Leystra, Dawn M. Albrecht, Mathew Brevard, Hemi Dimant, Robert Holt, Richard B. Halberg
Journal: Cancer Research (2018)

Abstract The ability to engineer models to express endogenous fluorophores has become an invaluable tool for studying tumor biology. The utility of these models is limited by the sensitivity and resolution of currently available imaging platforms. In vivo techniques enable longitudinal studies in which changes in fluorescent markers, tumors, and whole organs can be monitored over time. In vivo fluorescence suffers from poor sensitivity and resolution owing to the high absorption and scatter of light in biological tissue. While ex vivo fluorescence techniques allow for high resolution evaluation and characterization, these techniques typically only focus on a small samples of tissue instead of whole animals. Using a Cryo-Fluorescence Tomography (CFT) imaging approach, an imaging modality based on serial slicing and off-the-block fluorescence imaging, we can bridge the gap between in vivo and ex vivo resolution of the entire animal. As proof of concept, a novel mouse model expressing fluorophores was evaluated and characterized. Individual cells were labeled with either EGFP or tdTomato prior to tumor initiation. Intestinal tumors were induced through loss of Apc, the activation of PI3K, or both. Tumor heterogeneity and grade were assessed by traditional histopathology, individual-tissue CFT, and whole-animal CFT. Using CFT, serial sectioning and 3D-reconstruction of whole tissues were achieved at 10-50 µm resolution.The multi-resolution imaging approach of this model enabled visualization of molecular and histopathological information from a common in vivo resolution (1mm) to whole-tissue resolution. The CFT platform enabled a 3D data set at a resolution that was not previously available in traditional histopathology. CFT as a molecular tissue imaging technique has proven its utility as a complimentary assay in preclinical oncology studies allowing several characteristics of tumors including heterogeneity, growth, and malignant spread to be readily assessed. Citation Format: Mohammed Farhoud, Alyssa Leystra, Dawn M. Albrecht, Mathew Brevard, Hemi Dimant, Robert Holt, Richard B. Halberg. Multiple-resolution characterization of tumor heterogeneity as associated with disease progression in a mouse model of colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr LB-329.
Abstract 14294: Normal c-Myb Activity is Required for Bone Marrow-Derived Cells to Contribute to Arterial Remodeling Following Injury
Authors: Eric A Shikatani, Abdul Momen, Mansoor Husain
Journal: Circulation (2012)

Background: We showed that the c-Myb transcription factor regulates proliferation and differentiation of contractile vascular smooth muscle cells (VSMC) in adult mice and embryoid bodies respectively, the latter mediated by control over a specific hematopoietic and angiogenic progenitor. However, the definitive role of c-Myb in the proliferation and potential (trans)differentiation of bone marrow (BM)-derived vs. vessel resident cells in arterial remodeling following injury in the adult has eluded genetic examination due to the embryonic lethality of c-myb-/- mice from hematopoietic failure. To overcome this, we obtained a mouse with an ENU-generated point mutation in c-myb, resulting in a hypomorphic c-myb (h) allele with non-lethal loss of c-Myb activity. Methods & Results: Histology of common carotid arteries of 12 wk-old male c-mybh/h and c-mybwt mice was performed 14 days after wire denudation injury (CI). Uninjured vessels from wt and h/h mice did not differ in lumen, intima (I), and media (M) morph...
MALE SEXUAL DISORDERS
Journal: BMJ (1939)

In men sexual dysfunction refers to repeated impairment of normal sexual interest and/ or performance. What is regarded as normal sexual intercourse and what is thought to be impaired or unsatisfactory, depends in part on the expectations of the two individuals concerned. For example one couple may regard it as normal that the woman is regularly unable to achieve orgasm, whilst another may seek treatment. The problems of sexual dysfunction include those affecting sexual desire and sexual enjoyment, erectile impotence and premature ejaculation.
Neurological Symptoms in US Government Personnel in Cuba
Authors: Gerard J. Gianoli, James S. Soileau, P. Ashley Wackym
Journal: JAMA (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 13482: Urinary Concentrations of Isoflavones Are Not Associated With Risk of Incident Coronary Heart Disease: The Singapore Chinese Health Study
Authors: Mohammad Talaei, Rob M van Dam, Jian-Min Yuan, Bee Lan Lee, Choon Nam Ong, Woon Puay Koh, An Pan
Journal: Circulation (2014)

Introduction:  Data from prospective studies examining dietary intakes of soy isoflavones in relation to coronary heart disease (CHD) risk have been inconsistent, which may be partially due to the limitations in using questionnaires to assess isoflavone intakes or low consumption levels in western populations. Thus, we evaluated the relation between urinary concentrations of isoflavones and risk of incident CHD in middle-aged and older Chinese residing in Singapore.   Methods:  The study included 300 incident CHD cases (150 males and 150 females) and 300 matched controls nested in the Singapore Chinese Health Study, a prospective cohort of Chinese adults aged 45-74 years old enrolled in 1993-1998. Cases and controls were randomly selected from participants who provided morning spot urine samples during the first follow-up visit (1999-2004) and were free of CHD/stroke at the time of urine collection. The cases developed myocardial infarction confirmed by medical records or died of CHD up to 2010. The controls did not develop CHD during the follow-up and were matched by sex, dialect group, year of birth, year of recruitment and date of biospecimen collection. Urinary isoflavones were determined by high-performance liquid chromatography. Conditional logistic regression models were used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) with adjustment for potential confounders.   Results:  The mean concentration of urinary isoflavones was 5.92 and 5.51 nmol/mg creatinine in cases and controls, respectively. We did not observe significant association between urinary isoflavones and risk of CHD; the ORs (95% CIs) for the 2nd and 3rd tertiles were 0.88 (0.56-1.40) and 0.95 (0.62-1.43), respectively, compared to the 1st tertile (P-trend=0.95). No significant associations were found in men or women; the respective ORs (95% CIs) were 0.47(0.22-1.01) and 0.74 (0.39-1.41) in men; and 1.56 (0.81-3.00) and 1.23 (0.66-2.28) in women. No significant associations were observed for any of the individual isoflavone metabolites in urine: daidzein, geistein, glycitein and equol.   Conclusions:  The present study did not find a statistically significant association between urinary concentrations of total isoflavone or its metabolites and risk of incident CHD.
Choledochoduodenostomy for the treatment of portal cavernoma biliary stenosis to avoid bloody ERCP
Authors: Marie Napoléon, Jérôme Dumortier, Florian Rostain, Jérôme Rivory, Thierry Ponchon, Hélène Gimonet, Mathieu Pioche
Journal: Endoscopy (2018)

Endoscopic ultrasound-guided biliary drainage (EUS-BD) has emerged as an alternative to endoscopic retrograde cholangiopancreatography (ERCP) in cases of cannulation failures [1] [2] [3]. Two options are possible: choledochoduodenostomy or hepaticogastrostomy; however, these procedures are associated with higher morbidity, including bleeding [4] and bile leakage.
Characteristics of Tianeptine Exposures Reported to the National Poison Data System — United States, 2000–2017
Authors: Tharwat El Zahran, Joshua Schier, Emily Glidden, Stephanie Kieszak, Royal Law, Edward Bottei, Cynthia Aaron, Andrew King, Arthur Chang
Journal: MMWR Morbidity and Mortality Weekly Report (2018)

Tianeptine (marketed as Coaxil or Stablon) is an atypical tricyclic drug used as an antidepressant in Europe, Asia, and Latin America. In the United States, tianeptine is not approved by the Food and Drug Administration (FDA) for medical use and is an unscheduled pharmaceutical agent* (1). Animal and human studies show that tianeptine is an opioid receptor agonist (2). Several case studies have reported severe adverse effects and even death from recreational abuse of tianeptine (3-5). To characterize tianeptine exposures in the United States, CDC analyzed all exposure calls related to tianeptine reported by poison control centers to the National Poison Data System (NPDS)† during 2000-2017. Tianeptine exposure calls, including those for intentional abuse or misuse, increased across the United States during 2014-2017, suggesting a possible emerging public health risk. Most tianeptine exposures occurred among persons aged 21-40 years and resulted in moderate outcomes. Neurologic, cardiovascular, and gastrointestinal signs and symptoms were the most commonly reported health effects, with some effects mimicking opioid toxicity. A substantial number of tianeptine exposure calls also reported clinical effects of withdrawal. Among 83 tianeptine exposures with noted coexposures, the most commonly reported coexposures were to phenibut, ethanol, benzodiazepines, and opioids.
An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties
Authors: Sandra Loaiza, Silvia A. Ferreira, Tamara M. Chinn, Alex Kirby, Elena Tsolaki, Camilla Dondi, Katarzyna Parzych, Adam P. Strange, Laurent Bozec, Sergio Bertazzo, Martin A.B. Hedegaard, Eileen Gentleman, Holger W. Auner
Journal: Biomaterials (2018)

Cellular function depends on the maintenance of protein homeostasis (proteostasis) by regulated protein degradation. Chronic dysregulation of proteostasis is associated with neurodegenerative and age-related diseases, and drugs targeting components of the protein degradation apparatus are increasingly used in cancer therapies. However, as chronic imbalances rather than loss of function mediate their pathogenesis, research models that allow for the study of the complex effects of drugs on tissue properties in proteostasis-associated diseases are almost completely lacking. Here, to determine the functional effects of impaired proteostatic fine-tuning, we applied a combination of materials science characterisation techniques to a cell-derived, in vitro model of bone-like tissue formation in which we pharmacologically perturbed protein degradation. We show that low-level inhibition of VCP/p97 and the proteasome, two major components of the degradation machinery, have remarkably different effects on the bone-like material that human bone-marrow derived mesenchymal stromal cells (hMSC) form in vitro. Specifically, whilst proteasome inhibition mildly enhances tissue formation, Raman spectroscopic, atomic force microscopy-based indentation, and electron microscopy imaging reveal that VCP/p97 inhibition induces the formation of bone-like tissue that is softer, contains less protein, appears to have more crystalline mineral, and may involve aberrant micro- and ultra-structural tissue organisation. These observations contrast with findings from conventional osteogenic assays that failed to identify any effect on mineralisation. Taken together, these data suggest that mild proteostatic impairment in hMSC alters the bone-like material they form in ways that could explain some pathologies associated with VCP/p97-related diseases. They also demonstrate the utility of quantitative materials science approaches for tackling long-standing questions in biology and medicine, and could form the basis for preclinical drug testing platforms to develop therapies for diseases stemming from perturbed proteostasis or for cancer therapies targeting protein degradation. Our findings may also have important implications for the field of tissue engineering, as the manufacture of cell-derived biomaterial scaffolds may need to consider proteostasis to effectively replicate native tissues.
Abstract 5538: Arv7 validation in castrate resistant prostate tumors utilizing Rnascope <i>in situ</i> hybridization assay
Authors: Tanesha Cash-Mason, Jackson Wong, Martinez Martinez, Michael Sharp, Jayaprakash Karkera, John D. Alvarez, Gerald C. Chu, Shibu Thomas, Weiman Li, S. Ken Tian
Journal: Cancer Research (2018)

Abstract Background Androgen receptor splice variant 7 (ARV7) is a ligand-independent transcription factor associated with resistance to androgen deprivation therapy. There are efforts in development to monitor ARV7 expression during the treatment of patients with prostate cancer. Methods We evaluated ARV7 expression in primary and metastatic prostate adenocarcinomas tissues utilizing automated RNAScope (Advanced Cell Diagnostics) ISH assay, Immunohistochemistry (IHC) and quantitative PCR (qPCR). The novel RNAscope ISH assay was used for direct chromogenic visualization of RNA transcripts and to confirm positivity as an orthogonal approach to validate IHC and quantitative qPCR assay findings. We screened a total of 44 CRPC FFPE tumor samples by IHC and a subset of 28 (excluding controls) with the ISH and qPCR assays. Samples for qPCR were enriched for tumor by macrodissection. RNAScope Scoring Criteria was used to score ISH expression and raw Ct values were normalized to the RPL19 housekeeping gene for qPCR. Thresholds for each assay was established by comparing the findings with the other 2 assays. The data was calculated as ARV7 (+) or ARV7 (-) based on thresholds and compared to the other 2 assays to establish assay performance statistics. Results The ISH screening of 28 CRPC tumors resulted in 16 tumors with some level of positivity and approximately 10 tumors with significant positivity. Of the 44 CRPC tumors stained by IHC, 16 tumors showed various levels of ARV7 nuclear positivity and 8 tumors with strong positivity. Conclusions The detection of ARV7 was achieved in three independent assays. The correlation of three orthogonal data sets were used to identify positive and negative tissues. ISH has the potential for greater sensitivity compared to traditional IHC. The development of automated RNAscope ISH assay allows for more samples to run in a standardized manner with minimal inter-operator variably and requires less hands on-time gives consistently reproducible results. Citation Format: Tanesha Cash-Mason, Jackson Wong, Martinez Martinez, Michael Sharp, Jayaprakash Karkera, John D. Alvarez, Gerald C. Chu, Shibu Thomas, Weiman Li, S. Ken Tian. Arv7 validation in castrate resistant prostate tumors utilizing Rnascope in situ hybridization assay [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5538.
Mosaic mutations in <i>FGFR3</i> and <i>FGFR2</i> are associated with naevoid acanthosis nigricans or RAVEN (round and velvety epidermal naevus)
Authors: M. Larsabal, O. Cogrel, C. Caumont, M.‐H. Jegou, A. Taïeb, F. Morice‐Picard
Journal: British Journal of Dermatology (2018)