Seek and You Will Not Find: Ending the Hunt for Replication-Competent Retroviruses during Human Gene Therapy
Authors: Helen E. Heslop, Malcolm K. Brenner
Journal: Molecular Therapy (2017)

In this issue of Molecular Therapy, two articles1Cornetta K. Duffy L. Turtle C.J. Jensen M. Forman S. Binder-Scholl G. Fry T. Chew A. Maloney D.G. June C.H. Absence of Replication-Competent Lentivirus in the Clinic: Analysis of Infused T Cell Products.Mol. Ther. 2017; 26 (this issue): 280-288Abstract Full Text Full Text PDF PubMed Scopus (66) Google Scholar, 2Marcucci K.T. Jadlowsky J.K. Hwang W.-T. Suhoski-Davis M. Gonzalez V.E. Kulikovskaya I. Gupta M. Lacey S.F. Plesa G. Chew A. et al.Retroviral and Lentiviral Safety Analysis of Gene-Modified T Cell Products and Infused HIV and Oncology Patients.Mol. Ther. 2017; 26 (this issue): 269-279Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar and the related Letter to the Editor3Lyon D. Lapteva N. Gee A.P. Absence of Replication-Competent Retrovirus in Vectors, T Cell Products, and Patient Follow-Up Samples.Mol. Ther. 2017; 26 (this issue): 6-7Abstract Full Text Full Text PDF PubMed Scopus (9) Google Scholar provide extensive data on replication-competent retrovirus (RCR) and replication-competent lentivirus (RCL) monitoring in T cells genetically modified with retroviral or lentiviral vectors and in patient follow-up samples; no evidence for RCR or RCL has yet been found. These and previous studies4Bear A.S. Morgan R.A. Cornetta K. June C.H. Binder-Scholl G. Dudley M.E. Feldman S.A. Rosenberg S.A. Shurtleff S.A. Rooney C.M. et al.Replication-competent retroviruses in gene-modified T cells used in clinical trials: is it time to revise the testing requirements?.Mol. Ther. 2012; 20: 246-249Abstract Full Text Full Text PDF PubMed Scopus (48) Google Scholar, 5Cornetta K. Yao J. Jasti A. Koop S. Douglas M. Hsu D. Couture L.A. Hawkins T. Duffy L. Replication-competent lentivirus analysis of clinical grade vector products.Mol. Ther. 2011; 19: 557-566Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar argue that T cell products from an RCR- or RCL-negative vector lot need no longer be routinely subjected to this expensive and burdensome testing and that patient follow-up samples can be archived rather than tested. This change would significantly reduce the cost of these potent therapies without adversely affecting their safety. When integrating viruses such as retroviruses and lentiviruses were first used to transduce cellular products in clinical trials, investigators and regulatory agencies feared that a recombination event could lead to generation of a novel RCR or RCL during cell product manufacturing or in the patients receiving them. Such an event would increase genotoxicity and the risk of malignant transformation. A primate gene transfer study in the early 1990s, in which 3 of 10 animals who received hematopoietic stem cells transduced with a retroviral vector contaminated with replication-competent virus developed an aggressive T cell lymphoproliferative disease,6Donahue R.E. Kessler S.W. Bodine D. McDonagh K. Dunbar C. Goodman S. Agricola B. Byrne E. Raffeld M. Moen R. et al.Helper virus induced T cell lymphoma in nonhuman primates after retroviral mediated gene transfer.J. Exp. Med. 1992; 176: 1125-1135Crossref PubMed Scopus (481) Google Scholar confirmed this risk. The US Food and Drug Administration (FDA) therefore published guidance for monitoring clinical vector lots, manufactured cell products, and patients post-infusion using biologic or PCR-based testing to detect RCR or RCL. In the 25 years since this guidance was published, retroviral packaging cell line and vector designs have minimized the homology between vector and packaging cell sequences and have segregated packaging genes so that the generation of RCR is extremely unlikely. The segregation of vector components into four plasmids for lentiviral production has similarly ensured that, to date, RCL generation remains only a theoretical possibility.5Cornetta K. Yao J. Jasti A. Koop S. Douglas M. Hsu D. Couture L.A. Hawkins T. Duffy L. Replication-competent lentivirus analysis of clinical grade vector products.Mol. Ther. 2011; 19: 557-566Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar In addition, there are now long-term safety data from clinical trials, all of which used vector supernatants that were released for use only after negative tests for RCR/RCL. In 2012, Bear et al.4Bear A.S. Morgan R.A. Cornetta K. June C.H. Binder-Scholl G. Dudley M.E. Feldman S.A. Rosenberg S.A. Shurtleff S.A. Rooney C.M. et al.Replication-competent retroviruses in gene-modified T cells used in clinical trials: is it time to revise the testing requirements?.Mol. Ther. 2012; 20: 246-249Abstract Full Text Full Text PDF PubMed Scopus (48) Google Scholar published a multicenter review reporting that RCR screening using amplification in HEK293 cells and analysis with the S+L− focus assay were consistently negative from 30 master cell banks (MCBs) and 42 viral supernatant lots used in clinical trials at 4 centers. These vectors were used to generate 297 genetically-modified T cell products, all of which were themselves RCR negative. RCRs were also absent in 629 patient follow-up samples.4Bear A.S. Morgan R.A. Cornetta K. June C.H. Binder-Scholl G. Dudley M.E. Feldman S.A. Rosenberg S.A. Shurtleff S.A. Rooney C.M. et al.Replication-competent retroviruses in gene-modified T cells used in clinical trials: is it time to revise the testing requirements?.Mol. Ther. 2012; 20: 246-249Abstract Full Text Full Text PDF PubMed Scopus (48) Google Scholar In 2011, the National Gene Vector Biorepository (NGVB) similarly reported that 16 lentiviral vector products manufactured for clinical trial use had no evidence of RCL. The two articles and the Letter to the Editor in this issue of Molecular Therapy provide additional safety data to assuage concerns. A report from the NGVB discusses the screening of samples from 26 trials that used third-generation lentiviral vectors produced by transient transfection to genetically modify T cells.1Cornetta K. Duffy L. Turtle C.J. Jensen M. Forman S. Binder-Scholl G. Fry T. Chew A. Maloney D.G. June C.H. Absence of Replication-Competent Lentivirus in the Clinic: Analysis of Infused T Cell Products.Mol. Ther. 2017; 26 (this issue): 280-288Abstract Full Text Full Text PDF PubMed Scopus (66) Google Scholar Over 450 transduced T cell products manufactured for 375 subjects were screened for RCL, and all tests were negative. In addition, 296 of the subjects that received the T cell product were screened for RCL (using PCR for env) at least 1 month after infusion of the cell product. All tests were negative.1Cornetta K. Duffy L. Turtle C.J. Jensen M. Forman S. Binder-Scholl G. Fry T. Chew A. Maloney D.G. June C.H. Absence of Replication-Competent Lentivirus in the Clinic: Analysis of Infused T Cell Products.Mol. Ther. 2017; 26 (this issue): 280-288Abstract Full Text Full Text PDF PubMed Scopus (66) Google Scholar A second report from the University of Pennsylvania presents data between 2001 and 2017 for eight different investigational products used in clinical trial subjects with hematological malignancies, solid tumors, or HIV.2Marcucci K.T. Jadlowsky J.K. Hwang W.-T. Suhoski-Davis M. Gonzalez V.E. Kulikovskaya I. Gupta M. Lacey S.F. Plesa G. Chew A. et al.Retroviral and Lentiviral Safety Analysis of Gene-Modified T Cell Products and Infused HIV and Oncology Patients.Mol. Ther. 2017; 26 (this issue): 269-279Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar They analyzed 17 vector lots (15 lentiviral and 2 retroviral), 375 manufactured T cell products, and 308 patient samples after infusion. These analyses were also consistently negative.2Marcucci K.T. Jadlowsky J.K. Hwang W.-T. Suhoski-Davis M. Gonzalez V.E. Kulikovskaya I. Gupta M. Lacey S.F. Plesa G. Chew A. et al.Retroviral and Lentiviral Safety Analysis of Gene-Modified T Cell Products and Infused HIV and Oncology Patients.Mol. Ther. 2017; 26 (this issue): 269-279Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar Finally, a letter from Lyon et al.3Lyon D. Lapteva N. Gee A.P. Absence of Replication-Competent Retrovirus in Vectors, T Cell Products, and Patient Follow-Up Samples.Mol. Ther. 2017; 26 (this issue): 6-7Abstract Full Text Full Text PDF PubMed Scopus (9) Google Scholar updates the Baylor College of Medicine experience with 9 different retroviral vector lots tested for RCR. Again, all were negative. These vectors were used in 17 clinical trials to manufacture 266 T cell lines, all of which tested negative for RCR using a co-culture assay. A total of 549 patient samples from 220 patients infused with these products have been assayed, and all have been negative.3Lyon D. Lapteva N. Gee A.P. Absence of Replication-Competent Retrovirus in Vectors, T Cell Products, and Patient Follow-Up Samples.Mol. Ther. 2017; 26 (this issue): 6-7Abstract Full Text Full Text PDF PubMed Scopus (9) Google Scholar The results from these three studies as well as the previous 2012 report from Bear et al.2Marcucci K.T. Jadlowsky J.K. Hwang W.-T. Suhoski-Davis M. Gonzalez V.E. Kulikovskaya I. Gupta M. Lacey S.F. Plesa G. Chew A. et al.Retroviral and Lentiviral Safety Analysis of Gene-Modified T Cell Products and Infused HIV and Oncology Patients.Mol. Ther. 2017; 26 (this issue): 269-279Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar are summarized in Table 1. They provide compelling safety data attesting to the low risk of generating RCR or RCL with current retroviral PG13 packaging cells and vectors and third-generation lentiviral vectors using VSV-G and transient transfection, although we do not yet know whether newly emerging lentiviral packaging cell lines and viral envelopes will have similar characteristics. Nonetheless, these published studies show no measurable risk that any RCR-/RCL-negative supernatants will lead to unanticipated replicative recombinants following gene transfer. While testing genetically modified T cell products for RCL or RCR provides no discernible added value over testing of the retroviral or lentiviral vector, it imposes a substantial resource and financial burden—usually over $10,000 for each patient.Table 1Published reports of RCR/RCL TestingStudyVector ProductsGene-Modified T Cell LinesPatient Follow-Up StudiesRCLRCRRCLRCRRCLRCRBear et al., 20124Bear A.S. Morgan R.A. Cornetta K. June C.H. Binder-Scholl G. Dudley M.E. Feldman S.A. Rosenberg S.A. Shurtleff S.A. Rooney C.M. et al.Replication-competent retroviruses in gene-modified T cells used in clinical trials: is it time to revise the testing requirements?.Mol. Ther. 2012; 20: 246-249Abstract Full Text Full Text PDF PubMed Scopus (48) Google Scholar–42 vector lots negative–297 negative–629 negativeCornetta et al., 20175Cornetta K. Yao J. Jasti A. Koop S. Douglas M. Hsu D. Couture L.A. Hawkins T. Duffy L. Replication-competent lentivirus analysis of clinical grade vector products.Mol. Ther. 2011; 19: 557-566Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar26 vector lots negative–460 negative–296 negative–Marcucci et al., 20172Marcucci K.T. Jadlowsky J.K. Hwang W.-T. Suhoski-Davis M. Gonzalez V.E. Kulikovskaya I. Gupta M. Lacey S.F. Plesa G. Chew A. et al.Retroviral and Lentiviral Safety Analysis of Gene-Modified T Cell Products and Infused HIV and Oncology Patients.Mol. Ther. 2017; 26 (this issue): 269-279Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar15 vector lots negative2 vector lots negative351 negative24 negative288 negative20 negativeLyon et al., 20173Lyon D. Lapteva N. Gee A.P. Absence of Replication-Competent Retrovirus in Vectors, T Cell Products, and Patient Follow-Up Samples.Mol. Ther. 2017; 26 (this issue): 6-7Abstract Full Text Full Text PDF PubMed Scopus (9) Google Scholar–9 vector lots negative–266 negative–549 negativeTotal41 negative53 negative811 negative587 negative584 negative1495 negative Open table in a new tab The recent FDA approval of two genetically modified T cell products targeting CD19 with chimeric antigen receptors7Brenner M.K. Next Steps in the CAR Journey of a Thousand Miles.Mol. Ther. 2017; 25: 2226-2227Abstract Full Text Full Text PDF PubMed Scopus (3) Google Scholar, 8Rosenbaum L. Tragedy, Perseverance, and Chance - The Story of CAR-T Therapy.N. Engl. J. Med. 2017; 377: 1313-1315Crossref PubMed Scopus (90) Google Scholar, 9U.S. Food and Drug Administration. (2017). FDA approval brings first gene therapy to the United States: CAR T-cell therapy approved to treat certain children and young adults with B-cell acute lymphoblastic leukemia. https://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/ucm574058.htm.Google Scholar has led to intense debate about cost and accessibility,7Brenner M.K. Next Steps in the CAR Journey of a Thousand Miles.Mol. Ther. 2017; 25: 2226-2227Abstract Full Text Full Text PDF PubMed Scopus (3) Google Scholar a debate that will only become more intense as the number and reach of such therapies becomes broader. Eliminating the requirement for RCR/RCL testing of the genetically modified T cell product and routine testing of patient follow-up samples would significantly reduce the cost of developing and implementing these potent therapies without adversely affecting their safety. It is, therefore, now time to review the RCR and RCL guidelines that have been in place for 25 years. We should base our new practices on current evidence rather than past fears. H.E.H. and M.K.B. wrote the commentary. This work was supported by the National Cancer Institute (P50CA126752 and P01CA094237) and the Leukemia and Lymphoma Society.