tbuckley/cabot-search huggingface dataset:
Abstract 445: Characterizing The Cardiac Acetylome During Exercise
Authors: Laura Liu, Hasmik Keshishian, Xiaojun Liu, Tanya Svinkina, Carolin Lerchenmüeller, Glenn C Rowe, Mun C Chan, Steven A Carr, Anthony Rosenzweig
Journal: Circulation Research (2016)

Recent advances in mass spectrometry have led to in-depth characterization of both tissue and cellular acetylation profiles (or “acetylomes”). These studies reveal the abundance and importance of this post-translational modification not only in transcriptional control, but also in many signaling and metabolic pathways. In the heart, acetylation has been found to play a role in cardiac proliferation, differentiation, and hypertrophy in both developmental and pathological settings. To examine the role of acetylation in exercise-induced cardiac hypertrophy, we studied the expression of enzymes regulating these processes and then undertook a comprehensive proteomic analysis of cardiac proteome and acetylome in hearts from sedentary and exercised mice. We first characterized the expression profiles of 8 acetylases (MYST1-4, GCN5, PCAF, EP300, CREBBP) and 18 deacetylases (HDAC1-11 and SIRT1-7) in four different exercise protocols, including swimming, voluntary wheel run, acute treadmill run, and long-term treadmill run in aged mice. Then, using a proteomics workflow with greater sensitivity and higher coverage recently developed at the Broad Institute, we determined the acetylome of the swim exercised mouse heart (n=5) in comparison to sedentary animals (n=4). We concurrently assessed proteome and phospho-proteome profiles in the same set of samples. Confirmation of the presence of physiological hypertrophy and increased cardiac function with exercise was determined with echocardiography and morphometric analysis. With this method, we identified 4159 acetylation sites and 19,402 phosphorylation sites on 6615 proteins. Acetylated proteins were found to be involved in a diverse array of processes, but most prominently in metabolism and muscle contraction. Phospho-proteome analysis has highlighted the interplay and crosstalk between acetylation and phosphorylation. In summary, the cardiac acetylome is dynamically regulated by exercise and demonstrates major alterations in metabolic pathways. Understanding how physiological hypertrophy affects acetylation and the activity of downstream factors may provide novel insights into the cardioprotective role of exercise.
Goals of care documentation in advanced cancer.
Authors: Ingrid Harle, Safiya Karim, Christopher M. Booth
Journal: Journal of Clinical Oncology (2017)

58 Background: Documenting goals of care (GOC) is integral to patient centered care, decision making and quality performance. Studies show patients value conversations about GOC after receiving a terminal diagnosis. Positive effects have been demonstrated for survival, quality of life, disease management and healthcare savings. The ASCO QOPI recommends documentation within 3 visits from diagnosis of metastatic cancer. For hospital inpatients, GOC are more often integrated into care planning, as admissions frequently reflect changes in illness trajectory, yet documentation remains poor. This Quality Improvement (QI) initiative aims to increase GOC documentation for ambulatory patients and referral rates to Palliative Medicine. Methods: A retrospective cohort receiving palliative chemotherapy for metastatic lung, pancreas, colorectal and breast cancers during 2010-2015 were identified from pharmacy records at a Canadian Regional Cancer Centre. Inclusion criteria required a minimum of 4 clinic visits post treatment. Clinical notes of Oncologists and Palliative Medicine Physicians were reviewed for GOC documentation and referrals to Palliative Medicine. Baseline data determined improvement target rates. A clinical practice QI initiative applied passive and active phases, beginning May 2016. Passive phase included the development of a guideline, documentation template and referral process. The active phase is the implementation process (ongoing). Results: A cohort included 456 patients with metastatic cancer, 63% lung cancer. Oncologists documented GOC in 6% (26/456) and referred 47% to Palliative Medicine. GOC rate was 48% post-referral. Improvement target rates were set at 40% and for referrals 70% by March 2017. Analysis during the passive phase showed Oncologists’ GOC documentations increased to 15%. Analysis of the active phase is ongoing and preliminary results will be presented. Conclusions: Low rates of GOC documentation and referrals to Palliative Medicine persist. The passive phase of engaging physicians in the QI development positively influenced GOC documentation. This QI initiative will inform the sustainability of GOC documentation, Palliative Medicine referrals and standardization of institutional guidelines.
Oral tolerance modulates the skin transcriptome in mice with induced atopic dermatitis
Authors: J. O. Baek, J. R. Lee, J. Y. Roh, Y. Jung
Journal: Allergy (2017)

Abstract Defective gut immune reactions have been implicated in the development of atopic dermatitis ( AD ), whereas oral tolerance ( OT ), that is, the immune unresponsiveness induced by oral antigen administration, protects mice against AD . To investigate this protective role of OT , the transcriptomic profiles of skin were obtained by RNA sequencing from mice that were epicutaneously sensitized, orally tolerized prior to epicutaneous sensitization, or neither (control). Oral tolerance inhibited the upregulation of keratin‐ and allergic inflammation‐associated genes that occurred in the epicutaneously sensitized group. Compared to the controls, mice that were orally tolerized and epicutaneously sensitized showed an upregulation of genes that regulate inflammation or keratinocyte differentiation. Knocking down two of those genes, SCGB 1A1 and TSC 22D3 , upregulated Th2 inflammatory mediators and downregulated a cornified cell envelope‐related gene. Based on our findings, OT may protect skin against allergic inflammation by promoting the expression of genes that regulate Th2 inflammatory responses and skin barrier function.
Comparing antibiotic consumption between two European countries: are packages an adequate surrogate for prescriptions?
Authors: Laurence Watier, Philippe Cavalié, Bruno Coignard, Christian Brun-Buisson
Journal: Eurosurveillance (2017)

Defined daily doses (DDD) are the gold standard indicator for quantifying prescriptions. Since 2014, the European Centre for Disease Prevention and Control (ECDC) has also been using the number of packages per 1,000 inhabitants per day (ipd), as a surrogate for prescriptions, to report antibiotic consumption in the community and to perform comparisons between European Union (EU) countries participating in the European Surveillance of Antimicrobial Consumption Network (ESAC-Net). In 2015, consumption was reported to range across Europe from 1.0 to 4.7 packages per 1,000 ipd. Our analysis showed that consumption of antibiotics for systemic use per 1,000 ipd was on average 1.3 times greater in France than in Belgium when considering prescriptions in the numerator, 2.5 times greater when considering packages and 1.2 times greater when considering DDD. As long as the same metrics are used over time, antibiotic consumption data aggregated and disseminated by ECDC are useful for assessing temporal trends at the European level and within individual countries; these data may also be used for benchmarking across EU countries. While DDD - although imperfect - are the most widely accepted metric for this purpose, antibiotic packages do not appear suitable for comparisons between countries and may be misleading.
Canadian mother whose son died from strep throat sentenced to three years in prison
Authors: Owen Dyer
Journal: BMJ (2017)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following Canadian mother whose son died from strep throat sentenced to three years in prison Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
P1.05-044 The Impact of IASLC 8th Edition Updates for T-Classification for Lung Cancer in a US Population-Based Surgical Resection Cohort
Authors: Matthew Smeltzer, Nicholas Faris, Carrie Fehnel, Cheryl Houston-Harris, Meredith Ray, Yu-Sheng Lee, Meghan Meadows, Sam Signore, Chris Mutrie, Edward Robbins, Raymond Osarogiagbon
Journal: Journal of Thoracic Oncology (2017)
Abstract 16044: Understanding the Effect of Time on Chest Compression Generated Blood Flow
Authors: Joshua W Lampe, Tai Tin, George Bratinov, Wes Shoap, Yuxi Lin, Christopher L Kaufman, Henry Halperin, Lance B Becker
Journal: Circulation (2017)

Introduction: We reported that the pumping mechanism of mechanical chest compression (MCC) generated blood flow changed after ~10 min of chest compressions. We wanted to better understand the physi...
P3.01-017 Primary Lung Adenocarcinomas with Enteric Differentiation: A Retrospective Analysis
Authors: Laura Bonanno, Ilaria Attili, Nazarena Nannini, Paola Del Bianco, Stefano Frega, Giulia Pasello, Valentina Polo, Giulia Zago, Enrico Pizzirani, Pierfranco Conte, Stefano Indraccolo, Fiorella Calabrese
Journal: Journal of Thoracic Oncology (2017)
Abstract 14986: Nationwide Hospital Variation in the Use and Outcomes of Cardiac Stress Testing in Patients Presenting to the Emergency Department
Authors: Daniel W Mudrick, Sreekanth Vemulapalli, Christy R Houle, Rita Kristy, James R Spalding, Glenn Magee, Teresa Davis, Edward Lee, Durran Taylor, Therese M Kitt
Journal: Circulation (2016)

Introduction:  Hospitals vary significantly in rates of cardiac testing during initial evaluation of chest pain in the ED. This study examines hospital rates of cardiac re-testing and cardiac-related readmission 180 days post-discharge in a large national database (Premier).   Methods:  Initial inpatient and outpatient discharges between January 2009 and December 2014 were identified for patients 35 years or older first evaluated in the ED with a diagnosis consistent with acute coronary syndrome risk and cardiac biomarker testing. Patients were categorized based on occurrence of any noninvasive cardiac testing during their index visit and were summarized at the hospital level as a percentage. The highest (HQ) and lowest (LQ) quartiles of hospitals for both inpatient and outpatient encounters were identified based on the percentage of patients receiving any cardiac test (FIG). Multivariate models assessed the risk of cardiac re-testing and cardiac readmission within 180 days from index discharge.   Results:  There were 531 evaluable hospitals (134 each in HQ and LQ). Unadjusted mean rates of additional cardiac testing and cardiac readmissions were higher for HQ hospitals (FIG).  In the multivariate analyses, adjusting for hospital characteristics and case mix among inpatients, the HQ hospitals had a higher likelihood of cardiac re-testing (2.25, p    Conclusion:  After adjustment, hospitals with the highest rates of initial testing have higher rates of cardiac re-testing and cardiac-related readmissions compared to the lowest testing hospitals. Additional research is required to determine if these differences are influenced by hospital protocols, available testing-related resources or other factors not captured in this analysis.
Challenges, Perceptions, and Readiness of Oncology Clinicians for the MACRA Quality Payment Program
Authors: Chadi Nabhan, Yolaine Jeune-Smith, Peter Klinefelter, Ronan J. Kelly, Bruce A. Feinberg
Journal: JAMA Oncology (2017)

This study surveys oncologists, hematologists, and practice administrators to gain an understanding of their perceptions of and readiness to participate in the MACRA Quality Payment Program.
Hong Kong Consensus Statements for the Management of Unresectable Hepatocellular Carcinoma
Authors: Tom Tan-To Cheung, Philip Chong-Hei Kwok, Stephen Chan, Chin-Cheung Cheung, Ann-Shing Lee, Victor Lee, Hoi-Ching Cheng, Nam-Hung Chia, Charing C.N. Chong, Tak-Wing Lai, Ada L.Y. Law, Mai-Yee Luk, Chi Chung Tong, Thomas C.C. Yau
Journal: Liver Cancer (2018)

&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Hepatocellular carcinoma (HCC) is highly prevalent in Hong Kong due to the high prevalence of chronic hepatitis B infection. Liver cancer is the fourth most common cancer and the third most common cause of cancer death. Due to the high case load, there is a high level of local expertise in treating HCC, and the full spectrum of treatment modalities is available. This document summarizes how these modalities should be used based on the latest evidence. &lt;b&gt;&lt;i&gt;Summary:&lt;/i&gt;&lt;/b&gt; In 2 meetings held in early 2017, a multidisciplinary group of Hong Kong clinicians, including liver surgeons, interventional radiologists, clinical oncologists, and medical oncologists, met to update local consensus statements for management of HCC. These statements are based on the latest evidence and give detailed guidance on how to deploy these modalities, in particular for cases of HCC which are not suited to surgical resection. &lt;b&gt;&lt;i&gt;Key Messages:&lt;/i&gt;&lt;/b&gt; These statements give detailed information on how to decide if a patient is a candidate for resection, methods to improve candidacy for resection, and guidance for use of various nonsurgical interventions to manage patients ineligible for resection.
Patient-Reported Outcomes Following Treatment of Chronic Hepatitis C Virus Infection With Sofosbuvir and Velpatasvir, With or Without Voxilaprevir
Authors: Zobair M. Younossi, Maria Stepanova, Stuart Gordon, Stefan Zeuzem, Michael P. Mann, Ira Jacobson, Marc Bourliere, Curtis Cooper, Steven Flamm, K. Rajender Reddy, Kris Kowdley, Issah Younossi, Sharon Hunt
Journal: Clinical Gastroenterology and Hepatology (2017)
Prevalence of type I sensitization to alpha‐gal in forest service employees and hunters: Is the blood type an overlooked risk factor in epidemiological studies of the α‐Gal syndrome?
Authors: A. Cabezas‐Cruz, J. de la Fuente, J. Fischer, J. Hebsaker, E. Lupberger, G. Blumenstock, E. Aichinger, A. S. Yazdi, S. Enkel, R. Oehme, T. Biedermann
Journal: Allergy (2017)

All authors contributed equally to this correspondence and there is no conflict of interest. The authors declare no conflicts of interest. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels
Authors: M. Giulbudagian, G. Yealland, S. Hönzke, A. Edlich, B. Geisendörfer, B. Kleuser, S. Hedtrich, M. Calderón
Journal: Theranostics (2017)

Topical administration permits targeted, sustained delivery of therapeutics to human skin. Delivery to the skin, however, is typically limited to lipophilic molecules with molecular weight of < 500 Da, capable of crossing the stratum corneum. Nevertheless, there are indications protein delivery may be possible in barrier deficient skin, a condition found in several inflammatory skin diseases such as psoriasis, using novel nanocarrier systems.Water in water thermo-nanoprecipitation; dynamic light scattering; zeta potential measurement; nanoparticle tracking analysis; atomic force microscopy; cryogenic transmission electron microscopy; UV absorption; centrifugal separation membranes; bicinchoninic acid assay; circular dichroism; TNFα binding ELISA; inflammatory skin equivalent construction; human skin biopsies; immunohistochemistry; fluorescence microscopy; western blot; monocyte derived Langerhans cells; ELISA Results: Here, we report the novel synthesis of thermoresponsive nanogels (tNG) and the stable encapsulation of the anti-TNFα fusion protein etanercept (ETR) (~150 kDa) without alteration to its structure, as well as temperature triggered release from the tNGs. Novel tNG synthesis without the use of organic solvents was conducted, permitting in situ encapsulation of protein during assembly, something that holds great promise for easy manufacture and storage. Topical application of ETR loaded tNGs to inflammatory skin equivalents or tape striped human skin resulted in efficient ETR delivery throughout the SC and into the viable epidermis that correlated with clear anti-inflammatory effects. Notably, effective ETR delivery depended on temperature triggered release following topical application.Together these results indicate tNGs hold promise as a biocompatible and easy to manufacture vehicle for stable protein encapsulation and topical delivery into barrier-deficient skin.
Promise of Salivary MicroRNA for Assessing Concussion
Authors: William P. Meehan, Rebekah Mannix
Journal: JAMA Pediatrics (2017)

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Abstract 342: Comparison of Fractional Flow Reserve (FFR) and Cardiac Stress Testing in Prediction of Coronary Ischemia
Authors: Mahan Shahrivari, Preetham Kumar, Andrew K Kurklinsky, Michael S Levy, Brian P Shapiro, Issam D Moussa
Journal: Circulation (2013)

Introduction: Clinical outcomes of FFR-guided PCI have been superior to those of angiographically guided PCI, with or without prior cardiac stress testing. Based on these findings, we hypothesized that the accuracy of stress testing is inferior to that of FFR in determining the physiologic significance of angiographic coronary artery disease. Method: To that end, we studied 243 consecutive patients who underwent coronary angiography and FFR assessment between September 2010 and April 2013 at Mayo Clinic, Florida. Out of this group, we excluded 143 patients due to absence of stress testing in the preceding 6 months. We assessed demographics, coronary risk factors, symptoms, stress test variables, angiographic data, and FFR measurements. FFR values ≤0.80 were considered to be positive for ischemia. We evaluated the diagnostic performance of stress testing vs. FFR. Result: Out of the 100 patients included in the study, FFR was measured in the Left Main coronary artery in 3(3%), in the Left Anterior Descending artery (LAD) in 67(67%), in the left circumflex territory in 11(11%), in the Right Coronary Artery (RCA) in 18(18%). The diagnostic performance of stress testing vs. FFR is summarized in the table, with regards to presence of ischemia in any vascular territory and with regards to a specific vascular territory. Conclusion: Compared to FFR, cardiac stress testing has reasonable NPV but poor PPV in determining the presence of absence of overall ischemia in a given patient. Most concerning, however, is that stress testing has poor PPV and NPV when used to determine the site of ischemia in a specific vascular territory.
Abstract 16938: IW-1973 is a Potent Soluble Guanylate Cyclase Stimulator in vitro and in vivo With Extensive Tissue Distribution
Authors: Daniel P Zimmer, Jenny Tobin, Courtney Shea, Renee Sarno, Kimberly Long, Sarah Jacobson, Kim Tang, Peter Germano, James Wakefield, G-Yoon Jamie Im, James E Sheppeck, Albert T Profy, G. Todd Milne, Mark G Currie, Jaime L Masferrer
Journal: Circulation (2016)

Introduction: A number of diseases including hypertension, diabetes, heart failure, and pulmonary hypertension are associated with elevated reactive oxygen species, endothelial dysfunction, and red...
Clinical assessment of emotions in patients with cancer: Diagnostic accuracy compared to two reference standards.
Authors: Henk M.W. Verheul, Annemarie Braamse, W Aerts, A W Hoogendoorn, F J Snoek, Myra Ellen Van Linde, Joost Dekker
Journal: Journal of Clinical Oncology (2017)

24 Background: Previous research has suggested that clinical assessment of emotions in patients with cancer is suboptimal. Since well-trained and experienced doctors and nurses may differentiate between emotions that do or do not necessitate professional mental health care, we hypothesized that clinical assessment may be more accurate than previously concluded. This study aimed to evaluate the diagnostic accuracy of clinical assessment of emotions. Methods: The study was designed as a retrospective cohort study at a department of medical oncology. The clinical assessment of emotions by medical oncologists and nurses was derived from the patient file. Emotional distress and need for emotional care was assessed using the Distress Thermometer and Problem List. The sensitivity, specificity and diagnostic odds ratio of clinical assessment was calculated, with either (i) emotional distress or (ii) need for professional mental health care as a reference standard. Results: Clinical assessment resulted in notes on emotions in 42.2% of the patient files (n =185), with 36.2% of patients experiencing emotional distress and 10.8% expressing a need for professional mental health care. As expected, the sensitivity of clinical assessment of emotions was higher with ‘need for professional mental health care’ as reference standard, compared to ‘emotional distress’ as reference standard (0.85 versus 0.54, p &lt; 0.001). The diagnostic odds ratio showed a similar pattern (9.66 versus 2.10, p = 0.02). For specificity, equivalent results were obtained with the two reference standards (0.63 versus 0.64, p =0.63). Conclusions: Medical oncologists and nurses seem to differentiate between emotions that do or do not necessitate professional mental health care, resulting in a high sensitivity of clinical assessment of emotions in patients with cancer.