tbuckley/cabot-search huggingface dataset:
Tackling cancer burden in low-income and middle-income countries: Morocco as an exemplar
Authors: Farida Selmouni, Ahmed Zidouh, Latifa Belakhel, Catherine Sauvaget, Maria Bennani, Youssef Chami Khazraji, Abdellatif Benider, Christopher P Wild, Rachid Bekkali, Ibtihal Fadhil, Rengaswamy Sankaranarayanan
Journal: The Lancet Oncology (2018)
Re-intervention with endoscopic ultrasound-guided hepaticogastrostomy for unresectable hilar biliary drainage using a multipath occlusion balloon
Authors: Daisuke Uchida, Hironari Kato, Hiroyuki Okada
Journal: Endoscopy (2018)

Endoscopic ultrasound (EUS)-guided hepaticogastrostomy (EUS-HGS) and antegrade stenting (EUS-AS) have been developed as alternative biliary drainage methods; however, treating unresectable malignant hilar biliary strictures remains challenging [1] [2] [3]. We successfully performed re-intervention for malignant hilar biliary drainage after EUS-HGS using a multipath occlusion balloon.
Abstract 17114: How Long Should You Continue Resuscitation in Alternative Cardiopulmonary Resuscitation Using Venoarterial Extracorporeal Membrane Oxygenation?
Authors: Eisuke Kagawa, Dote Keigo, Masaya Kato, Shota Sasaki, Noboru Oda, Eiji Kunita, Michiaki Nagai
Journal: Circulation (2017)

Introduction: Durations of cardiopulmonary resuscitation (CPR) are inversely associated with favour outcome in pediatric and adult patients after cardiac arrest. However, little is known in extraco...
Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015
Authors: Maxime Bisseux, Jonathan Colombet, Audrey Mirand, Anne-Marie Roque-Afonso, Florence Abravanel, Jacques Izopet, Christine Archimbaud, Hélène Peigue-Lafeuille, Didier Debroas, Jean-Luc Bailly, Cécile Henquell
Journal: Eurosurveillance (2018)

BackgroundHuman enteric viruses are resistant in the environment and transmitted via the faecal-oral route. Viral shedding in wastewater gives the opportunity to track emerging pathogens and study the epidemiology of enteric infectious diseases in the community. Aim: The aim of this study was to monitor the circulation of enteric viruses in the population of the Clermont-Ferrand area (France) by analysis of urban wastewaters. Methods: Raw and treated wastewaters were collected between October 2014 and October 2015 and concentrated by a two-step protocol using tangential flow ultrafiltration and polyethylene glycol precipitation. Processed samples were analysed for molecular detection of adenovirus, norovirus, rotavirus, parechovirus, enterovirus (EV), hepatitis A (HAV) and E (HEV) viruses. Results: All wastewater samples (n = 54) contained viruses. On average, six and four virus species were detected in, respectively, raw and treated wastewater samples. EV-positive samples were tested for EV-D68 to assess its circulation in the community. EV-D68 was detected in seven of 27 raw samples. We collected data from clinical cases of EV-D68 (n = 17), HAV (n = 4) and HEV infection (n = 16) and compared wastewater-derived sequences with clinical sequences. We showed the silent circulation of EV-D68 in September 2015, the wide circulation of HAV despite few notifications of acute disease and the presence in wastewater of the major HEV subtypes involved in clinical local cases. Conclusion: The environmental surveillance overcomes the sampling bias intrinsic to the study of infections associated with hospitalisation and allows the detection in real time of viral sequences genetically close to those reported in clinical specimens.
Potential of butyrate to influence food intake in mice and men
Authors: Kristina S Fluitman, Madelief Wijdeveld, Max Nieuwdorp, Richard G IJzerman
Journal: Gut (2018)

In recent years, many studies have been dedicated towards elucidating the role of the intestinal microbiota in metabolic health. It was suggested that specific intestinal microbial compositions can either protect from—or contribute to obesity and metabolic diseases. A causal relation between the microbiota and host metabolic profile was demonstrated when faecal microbiota of lean and obese subjects was transferred into mice, which then exhibited corresponding phenotypes.1 Short chain fatty acids (SCFAs) have broadly been proposed as key mediators in the microbiota-induced metabolic effects on the host. They are produced by bacterial fermentation of otherwise indigestible nutrients. The most abundant SCFAs are propionate, acetate and butyrate. All have been extensively studied in murine models for their possible influence on colonic health, glucose and lipid metabolism, as well as appetite and energy expenditure. Although various mechanisms by which these SCFAs exert their effect were suggested,2–7 the precise pathways remain unclear.

In Gut , Li et al report on the mechanisms by which butyrate positively influences energy balance and protects from diet-induced obesity. They deduce that many of the butyrate-induced metabolic benefits are driven by a decrease in energy intake. In their first experiment, Li et al exposed overnight fasted mice to either intravenously administered butyrate or to butyrate administered via intragastric gavage. Interestingly, only intragastric administration affected feeding behaviour, reducing cumulative 24 hours food intake by 21%. Furthermore, it induced a marked shift in the activity of hypothalamic orexigenic and anorexic neurons. This demonstrates that butyrate administration affects central appetite regulation before butyrate enters …
Congenital Zika virus infection as a silent pathology with loss of neurogenic output in the fetal brain
Authors: Kristina M Adams Waldorf, Branden R Nelson, Jennifer E Stencel-Baerenwald, Colin Studholme, Raj P Kapur, Blair Armistead, Christie L Walker, Sean Merillat, Jay Vornhagen, Jennifer Tisoncik-Go, Audrey Baldessari, Michelle Coleman, Manjiri K Dighe, Dennis W W Shaw, Justin A Roby, Veronica Santana-Ufret, Erica Boldenow, Junwei Li, Xiaohu Gao, Michael A Davis, Jesica A Swanstrom, Kara Jensen, Douglas G Widman, Ralph S Baric, Joseph T Medwid, Kathryn A Hanley, Jason Ogle, G Michael Gough, Wonsok Lee, Chris English, W McIntyre Durning, Jeff Thiel, Chris Gatenby, Elyse C Dewey, Marian R Fairgrieve, Rebecca D Hodge, Richard F Grant, LaRene Kuller, William B Dobyns, Robert F Hevner, Michael Gale, Lakshmi Rajagopal
Journal: Nature Medicine (2018)
Brown adipose tissue thermogenic adaptation requires Nrf1-mediated proteasomal activity
Authors: Alexander Bartelt, Scott B Widenmaier, Christian Schlein, Kornelia Johann, Renata L S Goncalves, Kosei Eguchi, Alexander W Fischer, Günes Parlakgül, Nicole A Snyder, Truc B Nguyen, Oliver T Bruns, Daniel Franke, Moungi G Bawendi, Matthew D Lynes, Luiz O Leiria, Yu-Hua Tseng, Karen E Inouye, Ana Paula Arruda, Gökhan S Hotamisligil
Journal: Nature Medicine (2018)
In Vivo Expansion and Antitumor Activity of Coinfused CD28- and 4-1BB-Engineered CAR-T Cells in Patients with B Cell Leukemia
Authors: Zhi Cheng, Runhong Wei, Qiuling Ma, Lin Shi, Feng He, Zixiao Shi, Tao Jin, Ronglin Xie, Baofeng Wei, Jing Chen, Hongliang Fang, Xiaolu Han, Jennifer A. Rohrs, Paul Bryson, Yarong Liu, Qi-Jing Li, Bo Zhu, Pin Wang
Journal: Molecular Therapy (2018)

Several recent clinical trials have successfully incorporated a costimulatory domain derived from either CD28 or 4-1BB with the original CD3ζ T cell activating domain to form second-generation chimeric antigen receptors (CARs) that can increase the responsiveness and survival of CAR-engineered T (CAR-T) cells. However, a rigorous assessment of the individual benefits of these costimulatory components relative to the in vivo performance of infused T cells in patients is still lacking. Therefore, we have designed a study that allows us to investigate and compare the impact of different costimulatory signal domains on CAR-T cells in vivo. Patients with B cell leukemia were infused with a mixture of two types of CD19-specific CAR-T cells, individually bearing CD28 (28ζ) and 4-1BB (BBζ) costimulatory signaling domains. We found that such a clinical procedure was feasible and safe. Complete remission (CR) was observed in five of seven enrolled patients, with two patients exhibiting durable CR lasting more than 15 months. The in vivo expansion pattern of 28ζ and BBζ CAR-T cells varied significantly among individual patients. These results confirm a feasible method of comparing different CAR designs within individual patients, potentially offering objective insights that may facilitate the development of optimal CAR-T cell-based immunotherapies. Several recent clinical trials have successfully incorporated a costimulatory domain derived from either CD28 or 4-1BB with the original CD3ζ T cell activating domain to form second-generation chimeric antigen receptors (CARs) that can increase the responsiveness and survival of CAR-engineered T (CAR-T) cells. However, a rigorous assessment of the individual benefits of these costimulatory components relative to the in vivo performance of infused T cells in patients is still lacking. Therefore, we have designed a study that allows us to investigate and compare the impact of different costimulatory signal domains on CAR-T cells in vivo. Patients with B cell leukemia were infused with a mixture of two types of CD19-specific CAR-T cells, individually bearing CD28 (28ζ) and 4-1BB (BBζ) costimulatory signaling domains. We found that such a clinical procedure was feasible and safe. Complete remission (CR) was observed in five of seven enrolled patients, with two patients exhibiting durable CR lasting more than 15 months. The in vivo expansion pattern of 28ζ and BBζ CAR-T cells varied significantly among individual patients. These results confirm a feasible method of comparing different CAR designs within individual patients, potentially offering objective insights that may facilitate the development of optimal CAR-T cell-based immunotherapies.
IDENTIFICATION OF GENES AND VARIANT ALLELES ASSOCIATED WITH IRON OVERLOAD IN HEMOCHROMATOSIS HFE C282Y HOMOZYGOTES
Authors: Christine E. McLaren, Pradyumna D. Phatak, Paul C. Adams, V. Nathan Subramaniam, James C. Barton, Chad P. Garner, Lawrie W. Powell, Lyle C. Gurrin, Katrina J. Allen, Deborah A. Nickerson, Grant A. Ramm, Gregory J. Anderson, Gordon D. McLaren
Journal: American Journal of Hematology (2013)
Opioid Agonist and Antagonist Therapies Prevent Opioid Relapse
Authors: Anita Slomski
Journal: JAMA (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Correlation of PET/CT and CT RECIST response in GIST patients with PDGFRA D842V gene mutations treated with crenolanib.
Authors: Jennifer Madeline Matro, Jian Qin Yu, Michael C. Heinrich, Abhijit Ramachandran, Nora Ku, Margaret von Mehren
Journal: Journal of Clinical Oncology (2014)

10546 Background: Crenolanib (CP-868,596) is an orally bioavailable, selective inhibitor of platelet derived growth factor receptor (PDGFR) tyrosine kinase, with in vitro studies demonstrating activity against PDGFRA D842V mutant cell lines. A phase II study evaluated antitumor efficacy of crenolanib in patients (pts) with advanced gastrointestinal stromal tumor (GIST) with PDGFRA D842 related mutations and deletions. An exploratory objective evaluated metabolic response after 1 cycle of therapy as a predictor of RECIST response, so positron emission tomography/computerized tomography (PET/CT) data was available for a majority of pts. We present the results of this exploratory objective. Methods: Pts with advanced GIST with PDGFRA D842 related mutations and deletions, including D842V, with residual measurable disease were eligible for enrollment at 1 of 2 study sites. Pts with a baseline PET/CT and a follow-up PET/CT after 1 cycle were included in this analysis. One nuclear medicine specialist interpreted the scans and provided SUV estimates for index lesions at baseline and after 1 treatment cycle. RECIST measurements were provided by the local interpreting radiologist. Results: 12 pts treated with crenolanib had both a baseline and follow-up PET/CT after 1 cycle. Baseline FDG avidity was lower than expected for GIST based on published reports, with the exception of 1 pt who had average initial standardized uptake value (SUV) of 33. Average SUV excluding this pt was 4.6. The table shows PET metabolic response after 1 cycle and CT RECIST best response. 7 of 12 (58%) pts had non-concordant PET and CT responses, including one with partial response (PR) on PET but progressive disease (PD) on CT. Conclusions: In this small exploratory analysis, pts with PDGFRA D842 related mutations had lower than expected SUV activity on PET, and metabolic response did not predict response by RECIST. These results suggest that PET/CT may not be an optimal method for predicting, evaluating and following response among GIST pts with PDGFRA D842 related mutations. Clinical trial information: NCT01243346.Radiographic response. # of patients PET Metabolic Response CT RECIST 3 SD SD 2 PD PD 2* PD SD 2* SD PD 1* PR SD 1* PR PD 1* SD PR Abbreivation: SD, stable disease. *Indicates noncorrelation.
Infectious meningitis and encephalitis in adults in Denmark: a prospective nationwide observational cohort study (DASGIB)
Authors: J. Bodilsen, Merete Storgaard, L. Larsen, L. Wiese, J. Helweg-Larsen, A.-M. Lebech, C. Brandt, C. Østergaard, H. Nielsen
Journal: Clinical Microbiology and Infection (2018)
MRI and laboratory features and the performance of international criteria in the diagnosis of multiple sclerosis in children and adolescents: a prospective cohort study
Authors: Giulia Fadda, Robert A Brown, Giulia Longoni, Denise A Castro, Julia O'Mahony, Leonard H Verhey, Helen M Branson, Patrick Waters, Amit Bar-Or, Ruth Ann Marrie, E Ann Yeh, Sridar Narayanan, Douglas L Arnold, Brenda Banwell, Katherine Wambera, Mary B Connolly, Jerome Yager, Jean K Mah, Giullaume Sebire, David Callen, Brandon Meaney, Marie-Emmanuelle Dilenge, Anne Lortie, Daniela Pohl, Asif Doja, Sunita Venkateswaran, Simon Levin, E Athen MacDonald, David Meek, Ellen Wood, David Buckley, Mark Awuku, J Burke Baird, Virender Bhan, Rozie Arnaoutelis, Danusha Nandamalavan
Journal: The Lancet Child & Adolescent Health (2018)
Abstract 16015: Loss of Protective Autoimmunity in Atherosclerosis
Authors: Dennis Wolf, Teresa Gerhardt, Sara McArdle, Livia Baas, Melanie Vassallo, Thamotharampillai Dileepan, Bjarke E Hanson, Kevin Tse, Harley Tse, Alessandro Sette, Marc K Jenkins, Klaus Ley
Journal: Circulation (2016)

Background: In atherosclerosis, CD4+ T helper cells recognize auto-antigens including ApoB-100, the main protein in low-density lipoprotein (LDL). However, atherosclerosis-specific, auto-reactive C...
Lifetime air pollution exposure and asthma in a pediatric birth cohort
Authors: Mary B. Rice, Sheryl L. Rifas-Shiman, Augusto A. Litonjua, Matthew W. Gillman, Nicole Liebman, Itai Kloog, Heike Luttmann-Gibson, Brent A. Coull, Joel Schwartz, Petros Koutrakis, Emily Oken, Murray A. Mittleman, Diane R. Gold
Journal: Journal of Allergy and Clinical Immunology (2018)
Nonhealing Crusted Scalp Lesions in a 4-Year-Old Boy
Authors: Stephanie Nagel, Hagen Graf Einsiedel, Manfred Kunz
Journal: JAMA Dermatology (2018)

A 4-year-old boy presented with a 3-year history of nonhealing scalp lesions; physical examination revealed multiple disseminated erythematous papules, petechial hemorrhages, and yellowish crusts. What is your diagnosis?
Growth differentiation factor-15 is a new biomarker for survival and renal outcomes in light chain amyloidosis
Authors: Efstathios Kastritis, Ioannis Papassotiriou, Giampaolo Merlini, Paolo Milani, Evangelos Terpos, Marco Basset, Athanasios Akalestos, Francesca Russo, Erasmia Psimenou, Filia Apostolakou, Maria Roussou, Maria Gavriatopoulou, Evangelos Eleutherakis-Papaiakovou, Despina Fotiou, Dimitrios C. Ziogas, Elektra Papadopoulou, Constantinos Pamboucas, Meletios A. Dimopoulos, Giovanni Palladini
Journal: Blood (2018)
Genome-wide analysis of HIF-1 binding with chromatin immunoprecipitation and tiled microarray
Authors: Xiaobo Xia, Madeleine Lemieux, Wei Li, Edward Fox, X Shirley Liu, Andrew Kung
Journal: Cancer Research (2007)

2444   Hypoxia is a hallmark of tumors, and adaptation is mostly mediated by the transcription factors Hypoxia Inducible Factor-1 (HIF-1) and HIF-2. Hypoxia results in accumulation of HIFs, which bind to the cognate Hypoxia-Response Element (HRE) and transactivate target genes that contribute to many aspects of cancer progression. To better understand the mechanisms of HIF-mediated transactivation, we have mapped HIF-1 binding sites on a genome-wide scale using chromatin immunoprecipitation followed by analysis on tiled genomic arrays (ChIP-on-chip). Triplicate HIF-1 ChIP’ed DNA from both normoxic and hypoxic HepG2 cells are hybridized onto Affymetrix Human Tiling Arrays 2.0R, which contain probes across the whole genome with a 35 base resolution. Using model-based analysis tiling array algorithm, MAT, we have identified both known and novel HIF-1 binding sites and target genes. In contrast to estrogen receptor (ER), most HIF-1 binding sites are found in promoter regions. Integration of ChIP-on-chip with gene expression profiling and motif scanning data has revealed new details of HIF-1 mediated gene transactivation, as well as mechanisms of suppression of gene activation.
Hippo-mediated suppression of IRS2/AKT signaling prevents hepatic steatosis and liver cancer
Authors: Sun-Hye Jeong, Han-Byul Kim, Min-Chul Kim, Ji-Min Lee, Jae Ho Lee, Jeong-Hwan Kim, Jin-Woo Kim, Woong-Yang Park, Seon-Young Kim, Jae Bum Kim, Haeryoung Kim, Jin-Man Kim, Hueng-Sik Choi, Dae-Sik Lim
Journal: Journal of Clinical Investigation (2018)

Nonalcoholic fatty liver disease (NAFLD) is a major risk factor for liver cancer; therefore, its prevention is an important clinical goal. Ablation of phosphatase and tensin homolog (PTEN) or the protein kinase Hippo signaling pathway induces liver cancer via activation of AKT or the transcriptional regulators YAP/TAZ, respectively; however, the potential for crosstalk between the PTEN/AKT and Hippo/YAP/TAZ pathways in liver tumorigenesis has thus far remained unclear. Here, we have shown that deletion of both PTEN and SAV1 in the liver accelerates the development of NAFLD and liver cancer in mice. At the molecular level, activation of YAP/TAZ in the liver of Pten-/- Sav1-/- mice amplified AKT signaling through the upregulation of insulin receptor substrate 2 (IRS2) expression. Both ablation of YAP/TAZ and activation of the Hippo pathway could rescue these phenotypes. A high level of YAP/ TAZ expression was associated with a high level of IRS2 expression in human hepatocellular carcinoma (HCC). Moreover, treatment with the AKT inhibitor MK-2206 or knockout of IRS2 by AAV-Cas9 successfully repressed liver tumorigenesis in Pten-/- Sav1-/- mice. Thus, our findings suggest that Hippo signaling interacts with AKT signaling by regulating IRS2 expression to prevent NAFLD and liver cancer progression and provide evidence that impaired crosstalk between these 2 pathways accelerates NAFLD and liver cancer.
MicroRNA-15A AND MicroRNA-16 Increase in Circulating Pro-Angiogenia Cells (PACs) of Patients with Critical Limb Ischemia and Impair PAC Functions in vitro and in vivo
Authors: Gaia Spinetti, Orazio Fortunato, Andrea Caporali, Marco Meloni, Ilaria Floris, Betty Descamps, Giuseope Mangialardi, Micol Marchetti, Elena Sangalli, Ezio Faglia, Paolo Madeddu, Costanza Emanueli
Journal: Circulation (2012)
FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis
Authors: Tokio Matsuzaki, Oscar Alvarez-Garcia, Sho Mokuda, Keita Nagira, Merissa Olmer, Ramya Gamini, Kohei Miyata, Yukio Akasaki, Andrew I. Su, Hiroshi Asahara, Martin K. Lotz
Journal: Science Translational Medicine (2018)

Aging is a main risk factor for osteoarthritis (OA). FoxO transcription factors protect against cellular and organismal aging, and FoxO expression in cartilage is reduced with aging and in OA. To investigate the role of FoxO in cartilage, Col2Cre-FoxO1, 3, and 4 single knockout (KO) and triple KO mice (Col2Cre-TKO) were analyzed. Articular cartilage in Col2Cre-TKO and Col2Cre-FoxO1 KO mice was thicker than in control mice at 1 or 2 months of age. This was associated with increased proliferation of chondrocytes of Col2Cre-TKO mice in vivo and in vitro. OA-like changes developed in cartilage, synovium, and subchondral bone between 4 and 6 months of age in Col2Cre-TKO and Col2Cre-FoxO1 KO mice. Col2Cre-FoxO3 and FoxO4 KO mice showed no cartilage abnormalities until 18 months of age when Col2Cre-FoxO3 KO mice had more severe OA than control mice. Autophagy and antioxidant defense genes were reduced in Col2Cre-TKO mice. Deletion of FoxO1/3/4 in mature mice using Aggrecan(Acan)-CreERT2 (AcanCreERT-TKO) also led to spontaneous cartilage degradation and increased OA severity in a surgical model or treadmill running. The superficial zone of knee articular cartilage of Col2Cre-TKO and AcanCreERT-TKO mice exhibited reduced cell density and markedly decreased Prg4 In vitro, ectopic FoxO1 expression increased Prg4 and synergized with transforming growth factor-β stimulation. In OA chondrocytes, overexpression of FoxO1 reduced inflammatory mediators and cartilage-degrading enzymes, increased protective genes, and antagonized interleukin-1β effects. Our observations suggest that FoxO play a key role in postnatal cartilage development, maturation, and homeostasis and protect against OA-associated cartilage damage.
Serious Cardiovascular Events and Clinical Outcomes in Secondary Polycythemia: Efficacy of Conventional Phlebotomy and Proposed Hematocrit Target
Authors: Ajaz Bulbul, Emilio Paul Araujo-Mino, Matney Huber, Adrianna Bautista, Kyaw Zin Thein, Hatim Husain, Flowers Brittany, Sanjay Awasthi, Sai-Ching J Yeung, Masoud Khorsand
Journal: Blood (2017)
Detecting dysplasia using white light endoscopy or chromoendoscopy in ulcerative colitis patients without primary sclerosing cholangitis: A systematic review and meta-analysis
Authors: Saeed Azizi, Hussein Al-Rubaye, Mohammed Adil A. Turki, Muhammad R. Sameem Siddiqui, Arun P. Shanmuganandan, Bushra Ehsanullah, Ranjeet Brar, Al-Mutaz Abulafi
Journal: International Journal of Surgery (2018)