AB1290 Anti carbamylated protein in healthy systemic first-degree relatives of rheumatoid arthritis individuals: relation with clinical signs of arthritis
Authors: C. Romero-Sanchez, J. De Avila, L. Chila, M. Mahler, W. Bautista-Molano, J.M. Bello-Gualtero
Journal: Annals of the Rheumatic Diseases (2018)

<h3>Background</h3> Association studies in rheumatoid arthritis (RA) have been focused in the pre-clinical phases of the disease in asymptomatic individuals with higher risk to develop RA such as first-degree relatives (FDR).A new group of autoantibodies directed against carbamylated proteins (anti-Carp) have been discover in RA. <h3>Objectives</h3> To investigate the levels of anti-Carp in FDR and establish their association with the state of rheumatic condition and compare these variables with a control group of healthy individuals from general population <h3>Methods</h3> In total, 118 FDR and 118 healthy controls matched by age and gender were included. Rheumatologic (clinical and markers) and periodontal assessment was performed. Individuals selected were over 18 and less than 65 years old were studied in Bogota-Colombia.A complete medical history related to RA was obtained by rheumatologist. They were assessed by two periodontists calibrated. Cancer, autoimmune disease, recent infection, lactation or pregnancy, use of antibiotics in the last three months, diabetes, edentulous, periodontal and orthodontic treatment in the last six months, were exclusion criteria. Anti-carbamylated-fetal calf serum protein by ELISA (RUO, Inova Diagnostics, San Diego), RF (Rheumatoid Factor, Spinreact), ACPAs IgG/IgA(Inova, Diagnosis), Enzyme-linked immunosorbent assay for leptin, Adiponectin based on Luminex xMAP technology, high-sensitivity CRP and IL-6(Siemmens)by chemiluminescence and ESR were evaluated. An association analysis was performed to evaluate the relationship between anti-Carp levels and rheumatologic conditions using Chi square and Fisher test. Logistic regression model was performed to confirm these associations. All the analyses were performed with a level of significance of 95% <h3>Results</h3> In the FDR-group, seventy three percent were female with a mean age of 40.19±12.47 years, 22.2% were current smoker, leptin high in 26.27% and 4.23% and 16.9%, had positive RF and ACPAs respectively. Anti-Carp in 26.3%; Among the controls, 70.97% were women, with an average age of 41.0±12.2 years, leptin high in 12.7% (p=0.004), positive RF in 2.5% (p=0.71), ACPA 6.8% (p=0.013) and anti-Carp 15.3(p=0.027); in in the general group 20.8% were positive (49/236) for anti-Carp, of which 63.3% are FDR (OR=2.02 IC 95% 1.03–3.96, p=0.04), Likewise, a greater frequency of painful joint (62.2%) p=0.008 and swollen joint (79.2%) (OR=4.35 CI 95% 1.5–12.36,p=0.006) were observed. The model designed for FDR showed a significant association between anti-Carp positive with of at least 1 swollen joint adjusted to the presence of leptin levels above the moderate range (OR=2.84, CI 95% 1.20–6.71,p=0.017) <h3>Conclusions</h3> The Research effort aimed at the "risk" phases of RA has increased. That is, before the development of clinical signs of arthritis, to identify those individuals and modify environmental factors and pharmacological intervention. The findings show us a significantly higher frequency of anti-Carp in a cohort of FDR of RA patients compared to healthy controls no associations were found with other autoantibodies but a very interesting way with signs of early inflammation <h3>Reference</h3> [1] Alessandri C, et al. Clinical Exp Rheumatol, 2015;33:824–30 <h3>Acknowledgements</h3> <h3>Disclosure of Interest</h3> None declared