tbuckley/cabot-search huggingface dataset:
LINC01133 as ceRNA inhibits gastric cancer progression by sponging miR-106a-3p to regulate APC expression and the Wnt/β-catenin pathway
Authors: Xian-Zi Yang, Tian-Tian Cheng, Qing-Jun He, Zi-Ying Lei, Jun Chi, Zhen Tang, Quan-Xing Liao, Hong Zhang, Li-Si Zeng, Shu-Zhong Cui
Journal: Molecular Cancer (2018)

Gastric cancer (GC) is a common malignancy and frequent cause of cancer-related death. Long non-coding RNAs (lncRNAs) have emerged as important regulators and tissue-specific biomarkers of multiple cancers, including GC. Recent evidence has indicated that the novel lncRNA LINC01133 plays an important role in cancer progression and metastasis. However, its function and molecular mechanism in GC remain largely unknown. LINC01133 expression was detected in 200 GC and matched non-cancerous tissues by quantitative reverse transcription PCR. Gain- and loss-of-function experiments were conducted to investigate the biological functions of LINC01133 both in vitro and in vivo. Insights into the underlying mechanisms of competitive endogenous RNAs (ceRNAs) were determined by bioinformatics analysis, dual-luciferase reporter assays, quantitative PCR arrays, TOPFlash/FOPFlash reporter assay, luciferase assay, and rescue experiments. LINC01133 was downregulated in GC tissues and cell lines, and its low expression positively correlated with GC progression and metastasis. Functionally, LINC01133 depletion promoted cell proliferation, migration, and the epithelial–mesenchymal transition (EMT) in GC cells, whereas LINC01133 overexpression resulted in the opposite effects both in vitro and in vivo. Bioinformatics analysis and luciferase assays revealed that miR-106a-3p was a direct target of LINC01133, which functioned as a ceRNA in regulating GC metastasis. Mechanistic analysis demonstrated that miR-106a-3p specifically targeted the adenomatous polyposis coli (APC) gene, and LINC01133/miR-106a-3p suppressed the EMT and metastasis by inactivating the Wnt/β-catenin pathway in an APC-dependent manner. Our findings suggest that reduced expression of LINC01133 is associated with aggressive tumor phenotypes and poor patient outcomes in GC. LINC01133 inhibits GC progression and metastasis by acting as a ceRNA for miR-106a-3p to regulate APC expression and the Wnt/β-catenin pathway, suggesting that LINC01133 may serve as a potential prognostic biomarker and anti-metastatic therapeutic target for GC.
Virus-mimetic polymer nanoparticles displaying hemagglutinin as an adjuvant-free influenza vaccine
Authors: Chaeyeon Lee, Jonghwa Jeong, Taeheon Lee, Wei Zhang, Li Xu, Ji Eun Choi, Ji Hyun Park, Jae Kwang Song, Sinae Jang, Chi-Yong Eom, KyuHwan Shim, A.An Seong Soo, Young-Sun Kang, Minseok Kwak, Hyeong Jin Jeon, Jeung Sang Go, Yung Doug Suh, Jun-O Jin, Hyun-jong Paik
Journal: Biomaterials (2018)
Decreased Suppression and Increased Phosphorylated STAT3 in Regulatory T Cells are Associated with Benefit from Adjuvant PD-1 Blockade in Resected Metastatic Melanoma
Authors: David M. Woods, Rupal Ramakrishnan, Andressa S. Laino, Anders Berglund, Kelly Walton, Brian C. Betts, Jeffrey S. Weber
Journal: Clinical Cancer Research (2018)

Abstract Purpose: PD-1 blockade induces durable responses in patients with metastatic melanoma and prolongs relapse-free survival in patients with resected melanoma; however, current biomarkers do not consistently associate with patient responses. In this study, we investigated the impact of nivolumab therapy on peripheral blood regulatory T cells (Treg) and its relation to patient outcomes. Experimental Design: Peripheral blood Tregs and conventional CD4+ T cells from patients with resected high-risk melanoma treated with adjuvant nivolumab were assessed for gene expression changes by RNA-seq. Percentages of circulating Tregs and phosphorylated-STAT3 (pSTAT3) expression levels were assessed by flow cytometry and validated in an independent cohort of active disease patients. Suppressive function of Tregs was assessed in allogeneic mixed lymphocyte reactions. Results: Tregs from non-relapse patients had increased expression of proliferation associated genes. An increase in the proportion of circulating Tregs and pSTAT3 expression and a reduction in Treg-suppressive capacity were observed in non-relapsing, but not relapsing patient samples 13 weeks after starting treatment. In vitro blockade of PD-1 increased Treg percentages and pSTAT3 expression, and reduced Treg-suppressive function. PD-1 blockade also led to IL10 production by T cells, resulting in higher Treg proliferation. The addition of a STAT3 inhibitor ameliorated the increase in Tregs, enhanced suppressive function, and decreased T-cell IL10 production in vitro. Conclusions: These results demonstrate that induction of pSTAT3, reduced suppressive function, and a paradoxical increase in Treg proliferation are novel correlates of patient benefit from PD-1 blockade.
Guideline: USPSTF recommends exercise and multifactorial interventions for fall prevention in older adults
Authors: Melvyn Jones
Journal: Annals of Internal Medicine (2018)

Source Citation US Preventive Services Task Force, Grossman DC, Curry SJ, et al. Interventions to prevent falls in community-dwelling older adults: US Preventive Services Task Force recommendation statement. JAMA. 2018;319:1696-704. 29710141
Antibiotics and Acid-Suppressing Medications in Early Life and Allergic Disorders
Authors: Berthe C. Oosterloo, Arine M. Vlieger, Ruurd M. van Elburg
Journal: JAMA Pediatrics (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Review: Flexible vs restrictive ICU visiting policies reduce delirium and anxiety severity in patients
Authors: Daniel I. Steinberg
Journal: Annals of Internal Medicine (2018)

ACP Journal Club21 August 2018Review: flexible vs restrictive ICU visiting policies reduce delirium and anxiety severity in patientsDaniel I. Steinberg, MDDaniel I. Steinberg, MDIcahn School of Medicine at Mount Sinai, New York City, New York, USA (D.I.S.)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/ACPJC-2018-169-4-023 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Source CitationNassar Junior AP, Besen BA, Robinson CC, et al. Flexible versus restrictive visiting policies in ICUs: a systematic review and meta-analysis. Crit Care Med. 2018;46:1175-80. https://pubmed.ncbi.nlm.nih.gov/29642108Clinical Impact RatingsHospitalists: Geriatrics: Critical Care: References1 Davidson JE, Powers K, Hedayat KM, et al; American College of Critical Care Medicine Task Force 2004-2005, Society of Critical Care Medicine. Clinical practice guidelines for support of the family in the patient-centered intensive care unit: American College of Critical Care Medicine Task Force 2004-2005. Crit Care Med. 2007;35:605-22. [PMID: 17205007] Google Scholar2 Berwick DM, Kotagal M. Restricted visiting hours in ICUs: time to change. JAMA. 2004;292:736-7. [PMID: 15304472] Google Scholar3 West CP, Dyrbye LN, Erwin PJ, Shanafelt TD. Interventions to prevent and reduce physician burnout: a systematic review and meta-analysis. Lancet. 2016;388:2272-81. [PMID: 27692469] Google Scholar4 Rosa RG, Falavigna M, Robinson CC, et al; ICU Visits Study Group Investigators and the BRICNet. Study protocol to assess the effectiveness and safety of a flexible family visitation model for delirium prevention in adult intensive care units: a cluster-randomised, crossover trial (The ICU Visits Study). BMJ Open. 2018;8:e021193. [PMID: 29654049] Google Scholar Author, Article, and Disclosure InformationAffiliations: Icahn School of Medicine at Mount Sinai, New York City, New York, USA (D.I.S.)This article was published at Annals.org on 7 August 2018. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 21 August 2018Volume 169, Issue 4Page: JC23KeywordsAnxietyDeliriumIntensive care unitsNumber needed to treatObservational studiesOdds ratioQuality improvementRandomized trialsSystematic reviews ePublished: 21 August 2018 Issue Published: 21 August 2018 Copyright & PermissionsCopyright © 2018 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Abstract 15132: Amiodarone Treatments Improve the Inflammation in Heart Failure
Authors: Yusuke Tanigaito, Kayoko Sato, Kazutaka Kitamura, Atsushi Suzuki, Atsuko Futase, Tsuyoshi Shiga, Nobuhisa Hagiwara
Journal: Circulation (2014)

Introduction: Inflammation is deeply involved in heart failure (HF). In vitro studies showed that amiodarone blocked cytokine production from human mononuclear cells stimulated with LPS. In addition, the randomised trial showed that the low-dose amiodarone improved the mortality in severe congestive heart failure. Hypothesis: Amiodarone treatments improve the inflammation in HF. Methods/Results: Twenty-nine patients with HF (Age:57.1±16.9, Men:58.6%, LVEF:43.9±9.6%, NYHA2.0±0.9) including DCM, HCM, ARVC, ICM, and Valvular CM patients were studied, and compared with 64 normotensive healthy controls (Cont). BNP, hsCRP levels were higher in HF than Cont (P<0.001 and P<0.00001). In addition, inflammatory T cell cytokine, IL-6, IFNγ, and IL17, were increased in HF by CBA kit (P<0.001, P<0.05, and P<0.001, respectively). After amiodarone treatments for 1 month, the levels of IL-6, hsCRP, and BNP were significantly improved in HF patients. To investigate the beneficial amiodarone effects for inflammation in HF, ...
Physiological and druggable skipping of immunoglobulin variable exons in plasma cells
Authors: Mohamad Omar Ashi, Nivine Srour, Jean-Marie Lambert, Anne Marchalot, Ophélie Martin, Sandrine Le Noir, Eric Pinaud, Maria Victoria Ayala, Christophe Sirac, Jérôme Saulière, Jérôme Moreaux, Michel Cogné, Laurent Delpy
Journal: Cellular and Molecular Immunology (2018)
Prominent geneticist loses £3.5m grant over charges of bullying
Authors: Nigel Hawkes
Journal: BMJ (2018)

The Wellcome Trust has withdrawn a £3.5m (€3.9m; $4.5m) research grant to a scientist at the Institute of Cancer Research after allegations of bullying were made against her.

Nazneen Rahman, a prominent genetic researcher at the institute, resigned her position there after an investigation found that she had a case to answer. A law firm commissioned to investigate charges that were first laid against her by other staff members last November found sufficient evidence for the allegations to be reviewed by a disciplinary hearing.

Rahman announced her …
Abstract 19740: Intramuscular Lipid Accumulation is Reversed by a p62-lysosomal-independent Lipophagy Pathway
Authors: Truong N Lam, Romain Harmancey, Hernan Vasquez, Blaine Gilbert, Nina Patel
Journal: Circulation (2015)

Background and Hypothesis: In the mammalian heart, autophagy is an adaptive mechanism promoting the removal of protein aggregates or damaged cell organelles. A common feature of non-ischemic heart failure is lipid accumulation. We examined whether autophagy is impaired in the muscle in chronic lipid overload and investigated whether activation of autophagy protects myocytes from lipotoxicity. Methods: Rat L6 myocytes were incubated over a period of 6 days with or without long chain fatty acids (equimolar mixture of oleate and palmitate, 1.0mM). At day 6, an autophagic inhibitor (bafilomycin A1, 200 nM), or an autophagic activator (rapamycin, 1 μM), or both, were added for 48 hours. Intracellular triglyceride (TG) accumulation was assessed by Oil Red O staining and immunofluorescence and was quantified colorimetrically. Protein markers of autophagic flux (LC3 and p62) and cell death (Caspase 3 cleavage) were measured by immunoblotting. Results: Inhibition of autophagy using bafilomycin increased TG accumulation and also increased fatty acid-mediated cell death. Conversely, activation of autophagy using rapamycin reduced both intracellular lipid accumulation and cell death. Unexpectedly, treatment with both rapamycin and bafilomycin resulted in a decrease in lipid accumulation. Immunoblotting indicated p62 degradation (autophagic flux), while immunofluorescence revealed the colocalization of p62 with lipid droplets in these cells. These findings indicate the potential association of p62 with lipid droplet turnover, which is a novel pathway for the breakdown of lipid droplets in muscle cells. TG in the extracellular medium was also assessed. The same cells treated with rapamycin and bafilomycin had higher levels of extracellular TG compared to extracellular TG of cells with other treatments. The results suggest that the p62-mediated pathway for lipid degradation ultimately exports TG’s from the cell by exocytotic mechanisms. Conclusions: Autophagy promotes the clearance of lipids from myocytes and switches to an alternative, p62-mediated pathway of lipophagy and exocytosis in the context of chronic lipid overload. Moreover, lipophagy promotes metabolic adaptation and myocyte survival.
Abstract 18349: Molecular Classification of the Heart Transplant Endomyocardial Biopsy
Authors: Eleanor Chang, Gregory Fishbein, Maral Bakir, Galyna Bondar, Nicholas Jackson, David Liem, Silvio Litovsky, Jose Tallaj, Charlotte Starling, Peipei Ping, Elaine Reed, Mario Deng, Esteban Tabak, Martin Cadeiras
Journal: Circulation (2015)

Background: Endomyocardial Biopsy ( EMB) is the standard method to diagnose allograft rejection post HTx. While it is used to support medical decisions, insufficient diagnostic accuracy constitutes a fundamental limitation. The aim of this study is to develop a methodology that improves the classification of the EMB through a non-supervised evaluation of intramyocardial gene expression. Methods: Sixty-four heart tissues from 47 HTx recipients were subjected to genome wide mRNA sequencing. An unsupervised algorithm using optimal transport to mitigate batch effects and to filter confounding sources of variability was developed to identify molecular signatures of rejection. Linear Mixed Model identified genes statistically significant among the histology defined rejection groups. Weighted Gene Correlation Network Analysis (WGCNA) was used to establish 13 eigengene modules and module-clinical phenotype relationships. Gene Ontology was used for interpretation of the modules in their biological context. Results: O ur algorithm best classified the EMBs into 4 unsupervised clusters solely based on their gene expression. Statistical analysis showed a set of genes differentially expressed among groups defined by histology criteria. Top ranked genes were CLNK, TNFRSF10A, TRADD, CD2, and HLA-A. WGCNA revealed best trait-module correlation was observed between the classes defined by the unsupervised algorithm developed in this study followed by Histology. Figure 1 shows Module-Trait relationships, strength of association, significance and enriched biological process. Conclusion: We have developed an unsupervised algorithm that classifies the EMBs into 4 functionally distinctive categories. These categories are highly correlated with genomic modules defined by WGCNA and with the clinical phenotypes. To our knowledge, this is the first unsupervised classification of the EMBs. Further validation and performance will be provided at the time of presentation.
Abstract 17191: Comparison of Progression Rate Between Thoracic And Abdominal Aortic Diameter in a High-risk Asian Population for Aortic Aneurysm: a Prospective, 3 Year Follow-up Study With Non-contrast Computed Tomography
Authors: In-Jeong Cho, Hyuk-Jae Chang, Sang-Eun Lee, Chi Young Shim, Geu-Ru Hong, Namsik Chung
Journal: Circulation (2016)

Introduction: Screening strategies for aortic aneurysm according to risk factors and ethnicity are controversial. Hypothesis: This study aimed to investigate progression rate of thoracic and abdomi...
Response to Comment on Bril et al. Clinical and Histologic Characterization of Nonalcoholic Steatohepatitis in African American Patients. Diabetes Care 2018;41:187–192
Authors: Fernando Bril, Kenneth Cusi
Journal: Diabetes Care (2018)

We thank Pearlman et al. (1) for their interest in our article, which recommended screening for nonalcoholic steatohepatitis (NASH) in African American patients with nonalcoholic fatty liver disease (NAFLD), as steatohepatitis may occur in these patients as frequently as in Caucasian patients (2).

The authors mention a “recent large systematic review of 34 studies and over 350,000 subjects” where the risk of NASH in African Americans was lower than in Caucasians (relative risk 0.72 [95% CI 0.60–0.87]) (3). However, in a closer look, only 16,083 patients were part of the studies assessing the risk of NASH in patients with NAFLD (10 studies). Moreover, Pearlman et al. (1) missed that from this pool of studies, 8 out of the 10 included fewer African American patients than our study, ranging from 2 to …
Abstract 15915: Sudden Cardiac Death in Blacks versus Whites: A Closer Look at Coronary Pathology
Authors: Sandeep G Nair, Hiroyoshi Mori, Kyndaron Reinier, Aapo L Aro, Frank Kolodgie, Renu Virmani, Sumeet S Chugh
Journal: Circulation (2016)

Introduction: From a community-based study, we have recently reported a higher burden of sudden cardiac death (SCD) in blacks compared to whites, with significant differences in demographic and clinical profile. Coronary artery disease (CAD) is commonly associated with SCD, but due to low rates of postmortem evaluation in the community, there is limited information regarding possible race-related differences in pathophysiology of coronary disease. Methods: From an established, dedicated cardiac pathology database in Eastern US, we compared 133 consecutive black SCD subjects, to 195 white SCD subjects (2005-2010). Detailed clinical and autopsy data including cardiac and coronary gross and histopathology findings were compared. Results: Consistent with our published community-based findings, blacks were found to be significantly younger than whites (54.8 ± 14.6 yr vs 60.6 ± 14.1 yr; p <0.01), and more likely to be female (32% vs 23%; p =0.07). There were no significant differences in mean height (Blacks, 17...
Dual target strategy: a proposal to mitigate the risk of overtreatment and enhance patient satisfaction in rheumatoid arthritis
Authors: Ricardo J O Ferreira, Mwidimi Ndosi, Maarten de Wit, Eduardo José Ferreira Santos, Cátia Duarte, Johannes W G Jacobs, Pedro M Machado, Désirée van der Heijde, Laure Gossec, Jose A P da Silva
Journal: Annals of the Rheumatic Diseases (2018)

et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Abstract 14332: Factors Associated With Quality of Life in Atrial Fibrillation: Results From the ORBIT-AF Registry
Authors: Tiffany C Randolph, DaJuanicia N Simon, Gregg C Fonarow, Bernard J Gersh, Peter R Kowey, James A Reiffel, Gerald V Naccarelli, Paul S Chan, John A Spertus, Eric D Peterson, Jonathan P Piccini
Journal: Circulation (2015)

Background: With increasing options for treating atrial fibrillation (AF), it is important to understand and quantify patient symptoms and quality of life (QoL), as improving symptoms and maximizing QoL are primary treatment goals. However, little is known about the factors associated with QoL. Methods: The Atrial Fibrillation Effect on QualiTy-of-life (AFEQT) is a disease-specific QoL tool for AF, with domain and summary scores ranging from 0 (the worst health status) to 100. Using hierarchical pseudo-binomial regression, we evaluated factors associated with baseline AFEQT Summary and Subscale scores in the ORBIT AF registry. Adjusted associations were reported as odds ratios (OR) and 95% confidence intervals (CI). Results: AFEQT was assessed in 2,007 AF outpatients from 99 sites. Median age and interquartile range (IQR) was 76 (67-82); 43% were female; and the median CHA2DS2-VASC score was 4 (3-5). Median AFEQT summary score was 82 (67, 94). Median symptom score was 92 (78-100) and daily activity score was 77 (52-94). Female sex, younger age, new onset AF, higher heart rate, obstructive sleep apnea, and CAD were all associated with reduced QoL. (Table) Similar trends were seen for the symptoms subscale: female sex [OR 0.57 CI (0.48, 0.68)], younger age [OR 1.10 CI (1.06, 1.15)], and new onset AF [OR 0.55 CI (0.43, 0.70)] were associated with worse symptoms. Likewise, for the daily activities subscale, Other race [OR 0.54 CI (0.36-0.80)] and higher BMI [OR 0.98 CI (0.98-0.99)] were associated with decreased activity. Conclusions: In the largest community-based assessment of QoL in patients with AF, we found several patient factors to be associated with low QoL, including female sex, new onset AF, younger age, higher heart rate, and certain cardiopulmonary comorbidities. Understanding patient characteristics associated with worse QoL can be a foundation for eradicating disparities and tailoring treatment.
A collagen microchannel scaffold carrying paclitaxel-liposomes induces neuronal differentiation of neural stem cells through Wnt/β-catenin signaling for spinal cord injury repair
Authors: Xiaoran Li, Caixia Fan, Zhifeng Xiao, Yannan Zhao, Haimin Zhang, Jie Sun, Yan Zhuang, Xianming Wu, Jiajia Shi, Yanyan Chen, Jianwu Dai
Journal: Biomaterials (2018)
Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses
Authors: Elizabeth Stewart, Justina McEvoy, Hong Wang, Xiang Chen, Victoria Honnell, Monica Ocarz, Brittney Gordon, Jason Dapper, Kaley Blankenship, Yanling Yang, Yuxin Li, Timothy I. Shaw, Ji-Hoon Cho, Xusheng Wang, Beisi Xu, Pankaj Gupta, Yiping Fan, Yu Liu, Michael Rusch, Lyra Griffiths, Jongrye Jeon, Burgess B. Freeman, Michael R. Clay, Alberto Pappo, John Easton, Sheila Shurtleff, Anang Shelat, Xin Zhou, Kristy Boggs, Heather Mulder, Donald Yergeau, Armita Bahrami, Elaine R. Mardis, Richard K. Wilson, Jinghui Zhang, Junmin Peng, James R. Downing, Michael A. Dyer
Journal: Cancer Cell (2018)
Guideline: Insufficient evidence for vitamin D and/or calcium to prevent fractures in community-dwelling adults
Authors: Angela M. Cheung
Journal: Annals of Internal Medicine (2018)

Source Citation US Preventive Services Task Force, Grossman DC, Curry SJ, et al. Vitamin D, calcium, or combined supplementation for the primary prevention of fractures in community-dwelling adults: US Preventive Services Task Force recommendation statement. JAMA. 2018;319:1592-9. 29677309