tbuckley/cabot-search huggingface dataset:
Abstract 1719: Integrative genomic analysis of ovarian cancer cell lines points to EMT involvement in the development of cisplatin resistance
Authors: Alexandria Haslehurst, Madhuri Koti, Ricardo Vidal, Paul Park, Wenyu Jiang, Jeremy Squire, Harriet Feilotter
Journal: Cancer Research (2011)

Abstract Ovarian cancer is the 5th leading cause of cancer related death in women, and has the highest associated mortality rate of any of the gynecological cancers. Approximately 75-80% of women diagnosed will succumb to the disease. The high mortality rate associated with ovarian cancer is due to poor screening and detection methods, resulting in late stage diagnosis, and also to resistance of the tumours to chemotherapeutic drugs. Primary drug resistance occurs in about 20% of patients during their treatment phase while approximately 70-80% of women that initially respond to chemotherapy will develop drug resistance over the course of treatment or at the time of relapse. Being able to accurately predict which patients will not successfully respond to drugs is the first step in developing personalized medicine and preventing useless chemotherapy treatments. In order to discover biomarkers of cisplatin resistance, a multi-platform integrative approach was taken utilizing data from aCGH as well as miRNA and mRNA microarrays. Data were collected from a drug sensitive and drug resistant ovarian cancer cell line pair (A2780 and A2780cis). Working with the paired cell lines provided a homogeneous population with which to build an integration algorithm. Data analysis using GeneSpring and Nexus software showed an enrichment of genes involved in TGFβ and EGF signaling. These signaling pathways lead us to investigate the involvement of the epithelial to mesenchymal transition (EMT) in drug resistance. Interestingly, many of the key genes involved in the regulation of the EMT process were found to be upregulated in our mRNA expression data. Observations from cell culture work support EMT involvement. We have noted a slower growth rate and a change to a fibroblastic appearance in the resistant cells relative to their drug sensitive parents. Immunohistochemistry has also been utilized to show an increase in mesenchymal markers in the resistant cell line. In order to determine if these findings were representative of drug resistance in human ovarian cancer, expression data from cisplatin sensitive and resistant ovarian cancer tumours were mined specifically for these and other EMT genes. A large number of the same EMT genes were consistently shown to be upregulated in the drug resistant tumour tissues relative to the sensitive tumours. Together, these data suggest the potential involvement of the epithelial to mesenchymal transition in the development of cisplatin resistance in ovarian cancer. Further validation of these results in vitro is currently in progress. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1719. doi:10.1158/1538-7445.AM2011-1719
Abstract 2898: Intracellular dissociation of polymer coating from nanoparticles
Authors: Hongwei Chen, Peng Zou, Hayley Paholak, Duxin Sun
Journal: Cancer Research (2012)

Abstract Polymer-coated nanoparticles are widely used for drug delivery in cancer therapy. However, it is not known whether the polymer coating will be disrupted in the lipid bilayer or intracellular space. Our current work suggests that the polymer coating of inorganic nanoparticles is disrupted after internalization by cancer cells. Single dispersed red quantum dots (QDs) labeled with 5-FAM (green) (D = 28 nm) were incubated with cancer cells (PC-3) and imaged via fluorescence microscopy. Initially the 5-FAM-labeled polymer coating was attached to the QDs and its green fluorescence was quenched when the nanoparticles were internalized from 2 to 4 hrs, but 5-FAM-labeled polymer became separated from the QDs once they are inside lysosome of cells and its fluorescence suddenly visible from 8 hrs. The fluorescence ratio (5-FAM/QDs) were increased by 29-fold after 8 h incubation compared to 2 h. The fluorescence quenching effect was compared to free PEG-5-FAM mixed with QDs, which only exhibited a 44% decrease in 5-FAM quenching. In addition, the intracellular dissociation of polymer coating from QD loaded micelles (D = 300 nm) was also observed. Furthermore, when labeled amphiphilic polymeric micelles encapsulate hydrophobic iron oxide nanoparticles (IONPs) with the hydrophobic dye BODIPY-TMR (red), double quenching was observed by encapsulated nanoparticles and the hydrophobic region inside the micelles by 8-fold fluorescence reduction. The BODIPY-TMR-labeled nanoparticles also showed polymer dissociation inside of cancer cells after 24 h incubation. These results suggest that polymer coating is stable in the lipid bilayer and it is dissociated from nanoparticles in the lysosome of cancer cells. These data will provide guidance for intracellular drug delivery using polymer coated nanoparticles. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2898. doi:1538-7445.AM2012-2898
The Efficacy of Terazosin, Finasteride, or Both in Benign Prostatic Hyperplasia
Authors: Herbert Lepor, William O. Williford, Michael J. Barry, Michael K. Brawer, Christopher M. Dixon, Glenn Gormley, Clair Haakenson, Madeline Machi, Perinchery Narayan, Robert J. Padley
Journal: New England Journal of Medicine (1996)

Men with benign prostatic hyperplasia can be treated with α1-adrenergic–antagonist drugs that relax prostatic smooth muscle or with drugs that inhibit 5α-reductase and therefore reduce tissue androgen concentrations. However, the effects of the two types of drugs have not been compared.
AB0049 Human Type 1 Innate Lymphoid Cells Accumulate in the Inflamed Synovium in Spondyloarthritis
Authors: N. Yeremenko, T. Noordenbos, I. Blijdorp, H. Hreggvidsdottir, K. Germar, J.H. Bernink, H. Spits, D. Baeten
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> Spondyloarthritis (SpA) is a major form of chronic inflammatory arthritis characterized by inflammation of axial and peripheral joints and by pathologic new bone formation leading to ankylosis. Consistent genetic, experimental, and clinical evidence indicates that IL-23/IL-17 immune axis plays a pivotal role in the pathophysiology SpA. It remains, however, unknown which IL-23 responsive cells are the major cellular source of IL-17 in SpA. Innate lymphoid cells (ILCs) are an emerging family of innate immune cells that produce various cytokines, including IL-17 and IL-22, and play critical roles in regulation of inflammation and tissue remodeling. <h3>Objectives</h3> In this study we investigated the presence and phenotype of ILCs in the peripheral blood and inflamed peripheral joints of patients with SpA. <h3>Methods</h3> Paired peripheral blood (PB), synovial fluid (SF) and synovial tissue (ST) were obtained from SpA patients with actively inflamed knee joints. ILCs (lineage negative, CD45<sup>+</sup>CD127<sup>+</sup> CD161<sup>+</sup>) were analysed by flow cytometry. <h3>Results</h3> ILCs were present in all three compartments of patients with SpA. Analysis of ST revealed a significantly increased frequency of total ILCs in the joint compared with PB ((median (IQR) 0.37 (0.12-1.12)% of the lymphocyte population in ST versus 0.06 (0.04-0.09) % in PB, p=0.016). Immunophenotyping of ILC subsets showed a statistically significant increase in the frequency of ILC1 (CRTH2<sup>-</sup>NKp44<sup>-</sup>ckit<sup>-</sup>) in ST (37.8%; 74.43-20.47%) versus SF (7.27%; 0.6-25.1%, p=0.008) and PB (3.45%; 1.45-9.25%, p=0.004). The second most prominent ILCs in the joint were NCR-negative ILC3 (CRTH2<sup>-</sup>NKp44<sup>-</sup>ckit<sup>+</sup>), composing 33.45% (9.54-50.64%) of the total ILCs. NCR-positive ILC3 (CRTH2<sup>-</sup>NKp44<sup>+</sup>ckit<sup>+</sup>) and ILC2 (CRTH2<sup>+</sup>) populations were present in synovium at lower frequencies. <h3>Conclusions</h3> We observed an absolute and relative enrichment of both ILC1 and NCR-negative ILC3 in the inflamed ST of patient with spondyloarthritis as compared to PB and SF. As studies in other tissues such as gut and tonsil revealed that these IL-23 responsive ILC subsets can be an important source of IL-17 and /or IL-22 (1, 2), we will further investigate the cytokine production by these synovial ILCs. <h3>References</h3> Geremia et al. J Exp Med. 2011 208(6):1127-1133 Bernink et al. Nature Immunology 14(3):221-229 <h3>Disclosure of Interest</h3> None declared
Abstract LB-289: A novel role for the p19ARF tumor suppressor in chromosome segregation
Authors: Eric MC Britigan, Beth A. Weaver
Journal: Cancer Research (2012)

Abstract The p19ARF tumor suppressor (p14ARF in humans) is part of the ink4a locus and has been implicated in a variety of cancers. The traditional role for p19ARF has been in the stabilization of p53, a protein which is inactivated or mutated in 50% of tumors. However, it has been suggested that p19ARF has roles outside of this pathway. Evidence for this comes from mouse model studies that found mice lacking p19ARF and p53 develop a wider tumor spectrum and are more likely to have multiple tumors than mice lacking p53 alone. We have found that loss of p19ARF in mouse embryonic fibroblasts (MEFs) leads to a weakened mitotic checkpoint and aneuploidy. The mitotic checkpoint is the major cell cycle checkpoint regulating chromosome segregation and progression through mitosis. Defects in chromosome segregation produce progeny that are aneuploid, meaning they contain an abnormal chromosome number that deviates from a multiple of the haploid. The majority of cancers are aneuploid, which suggests that a dysfunctional mitotic checkpoint could play a role in tumorigenesis or cancer progression. In addition to a weakened mitotic checkpoint, loss of p19ARF leads to other mitotic defects including multipolar spindles, supernumerary centrosomes, and lagging chromosomes in anaphase. Future work will define the mechanism by which p19ARF functions in mitosis and establish an additional role in which p19ARF acts as a tumor suppressor. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-289. doi:1538-7445.AM2012-LB-289
Cardiovascular Risk Factor Targets and Cardiovascular Disease Event Risk in Diabetes: A Pooling Project of the Atherosclerosis Risk in Communities Study, Multi-Ethnic Study of Atherosclerosis, and Jackson Heart Study
Authors: Nathan D. Wong, Yanglu Zhao, Rohini Patel, Christopher Patao, Shaista Malik, Alain G. Bertoni, Adolfo Correa, Aaron R. Folsom, Sumesh Kachroo, Jayanti Mukherjee, Herman Taylor, Elizabeth Selvin
Journal: Diabetes Care (2016)

OBJECTIVE Controlling cardiovascular disease (CVD) risk factors in diabetes mellitus (DM) reduces the number of CVD events, but the effects of multifactorial risk factor control are not well quantified. We examined whether being at targets for blood pressure (BP), LDL cholesterol (LDL-C), and glycated hemoglobin (HbA1c) together are associated with lower risks for CVD events in U.S. adults with DM. RESEARCH DESIGN AND METHODS We studied 2,018 adults, 28–86 years of age with DM but without known CVD, from the Atherosclerosis Risk in Communities (ARIC) study, Multi-Ethnic Study of Atherosclerosis (MESA), and Jackson Heart Study (JHS). Cox regression examined coronary heart disease (CHD) and CVD events over a mean 11-year follow-up in those individuals at BP, LDL-C, and HbA1c target levels, and by the number of controlled risk factors. RESULTS Of 2,018 DM subjects (43% male, 55% African American), 41.8%, 32.1%, and 41.9% were at target levels for BP, LDL-C, and HbA1c, respectively; 41.1%, 26.5%, and 7.2% were at target levels for any one, two, or all three factors, respectively. Being at BP, LDL-C, or HbA1c target levels related to 17%, 33%, and 37% lower CVD risks and 17%, 41%, and 36% lower CHD risks, respectively (P &amp;lt; 0.05 to P &amp;lt; 0.0001, except for BP in CHD risk); those subjects with one, two, or all three risk factors at target levels (vs. none) had incrementally lower adjusted risks of CVD events of 36%, 52%, and 62%, respectively, and incrementally lower adjusted risks of CHD events of 41%, 56%, and 60%, respectively (P &amp;lt; 0.001 to P &amp;lt; 0.0001). Propensity score adjustment showed similar findings. CONCLUSIONS Optimal levels of BP, LDL-C, and HbA1c occurring together in individuals with DM are uncommon, but are associated with substantially lower risk of CHD and CVD.
A Comparison of Noninvasive Positive-Pressure Ventilation and Conventional Mechanical Ventilation in Patients with Acute Respiratory Failure
Authors: Massimo Antonelli, Giorgio Conti, Monica Rocco, Maurizio Bufi, Roberto Alberto De Blasi, Gabriella Vivino, Alessandro Gasparetto, Gianfranco Umberto Meduri
Journal: New England Journal of Medicine (1998)

The role of noninvasive positive-pressure ventilation delivered through a face mask in patients with acute respiratory failure is uncertain. We conducted a prospective, randomized trial of noninvasive positive-pressure ventilation as compared with endotracheal intubation with conventional mechanical ventilation in 64 patients with hypoxemic acute respiratory failure who required mechanical ventilation.
SP0140 Neutrophil extracellular traps
Authors: A. Zychlinsky
Journal: Annals of the Rheumatic Diseases (2013)

Neutrophils are one of the first lines of defence of the immune system against microbes. These cells kill microorganisms effectively by phagocytosis and by the formation of extracellular structures, called Neutrophil Extracellular Traps (NETs). NETs are made of chromatin and specific neutrophil proteins and are released after a unique cell death program that requires the production of radical oxygen species (ROS) and the relocation of neutrophil elastase to the nucleus. NETs help limit and control infection and also can activate the acquired immune system. Thus, formation of NETs appears to be necessary for an efficient clearing of microbes but can also initiate and exacerbate autoimmune responses. <h3>Disclosure of Interest</h3> None Declared
1134 PREMATURE TREATMENT DISCONTINUATION OF PEG-IFNa-2A/RBV DUE TO GOOD VIRAL RESPONSES AND INSUFFICIENT VIRAL RESPONSES AMONG HCV GENOTYPE 1, 2 AND 3 PATIENTS FROM PROPHESYS
Authors: A. Mangia, T. Bányai, G. De Bartolomeo, F. Habersetzer, J.-P. Mulkay, D. Ouzan, G. Parruti, N. Passariello, J. Gervain, A.-J. Remy, M. Rizzetto, M.L. Shiffman, A.D. Tice, M. Schmitz, F. Tatsch, M. Rodriguez-Torres
Journal: Journal of Hepatology (2012)
P514 TOLERANCE AND EFFICIENCY OF PORTOPULMONARY HYPERTENSION TREATMENT WITH OR WITHOUT LIVER TRANSPLANTATION
Authors: E. Bardou-Jacquet, C. Chabanne, C. Camus, M. Latournerie, P. Houssel, C. Jezequel, K. Boudjema, D. Guyader
Journal: Journal of Hepatology (2014)
Value ER, PR and HER Status as Independent Prognostic Factor of Local Recurrence after Breast Conserving Surgery.
Authors: V. Ostapenko, S. Bruzas, A. Mudenas, J. Sabonis, J. Kurtinaitis, A. Jackevicius, A. Ostapenko
Journal: Cancer Research (2009)

Abstract Aim : Evaluate value ER, PR and HER2 status as independent prognostic factor of local recurrence after breast conserving surgery Methods and materials: The retrospective study of 537 breast cancer patients stage I,II,III which were combined treated in Institute Oncology, Vilnius University in 2005-2006.was made The surgery treatment was used as primarily selection criteria, the data on vital status of all patients was summarized by December, 2008. The median follow-up time was 33 month. Pts age was from 29 years to 76 years. Premenopausal pts (&amp;lt;50 years) - 181 pts, postmenopausal – 356 pts. All pts underwent Breast conserving surgery, irradiation and chemohormono therapy. Distribution of pts according pathological tumor size "pT" was pT0 - 5 pts (1,0%), pT1 - 312 pts (58,1%), pT2 - 208 pts (38,7%), pT3 - 12 pts (2,2%). Distribution of pts according to nodal status "pN" was: "N0" - 333 pts (62,1%), "N1"- 172 pts (32%), "N2" - 30 pts (5,8%), "N3" - 2 pts (0,3%). Distribution of pts according differentiation degree "G" was “G1"- 100 (18,6%), "G2"- 266 (49,7%), "G3" - 171 (31,7%). Pts distribution according ER receptors positive tumors was ER strong (score &amp;gt;=10) - 166 pts (30,9%), medium (score 6-9) - 146 pts (27,2%), week (scrore &amp;lt;=5) - 72 pts (13,4%), no reaction - 148 pts (27,6%). Pts distribution according PR receptors positive tumors was PR strong (score &amp;gt;=10) - 141 pts (26,6%), medium (score 6-9) - 125 pts (23,3%), week (scrore &amp;lt;=5) - 84 pts (15,6%), no reaction - 182 pts (33,9%). Pts distribution according HER2 receptors (3+) - 117 pts (21,8%), HER2 receptors (2+) - 116 pts (21,8%), HER2 receptors (1+) - 188 pts (35,0%), HER2 receptors no reaction - 110 pts (20,5%).Results: Local recurrence rate was calculated according Nelson-Aalen cumulative hazard estimate (analysis time 4 years) and was equal to 8 %.Local recurrence rate according ER-, PR-, HER2 0 vs ER+, PR+, HER2 3+ was equal to 14% vs 4,5%.In 63,5% of all local recurrence ER, PR were negative “0 “,in 40.9% of all local recurrences HER2 was positive (3+) Conclusion: According to our preliminary results ER, PR and HER2 status are independent prognostic factor of local recurrence after breast. Citation Information: Cancer Res 2009;69(24 Suppl):Abstract nr 6047.
Reply to Ustundag et al.
Authors: K. de Jong, J.-W. Poley, J. van Hooft, M. Visser, M. Bruno, P. Fockens
Journal: Endoscopy (2011)

In the comment on our recent paper [1] describing the technical results and complications of fine needle aspiration (FNA) of pancreatic cysts, Ustundag et al. highlight several important issues pertaining to the role of endoscopy-guided FNA in the diagnostic work-up of pancreatic cysts.
18F-FDG PET/CT in Anti-LGI1 Encephalitis
Authors: Sohyun Park, Hongyoon Choi, Gi Jeong Cheon, Keon Wook Kang, Dong Soo Lee
Journal: Clinical Nuclear Medicine (2014)

Anti-LGI1 encephalitis is a subgroup of autoimmune encephalitis, characterized by memory impairments, seizures, and behavioral problems. The diagnosis can be made by clinical manifestation with a help of serum autoantibody test. There was lack of imaging studies to evaluate and monitor the disease activity by anatomical and functional information. Here, we report serial F-FDG PET/CT findings in a patient with anti-LGI1 encephalitis. Intense F-FDG uptake was initially noted in bilateral limbic system at active disease status, and then decreased and eventually normalized according to the clinical improvement after treatment. F-FDG PET/CT can be used to evaluate treatment response of encephalitis.
PROGNOSTIC IMPACTS OF ATRIAL FIBRILLATION STATUS IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION: A STUDY OF JAPANESE REGISTRY OF ACUTE MYOCARDIAL INFARCTION DIAGNOSED BY UNIVERSAL DEFINITION
Authors: Nobuaki Kokubu, Masaharu Ishihara, Masashi Fujino, Hisao Ogawa, Koichi Nakao, Satoshi Yasuda, Yukio Ozaki, Teruo Noguchi, Kazuo Kimura, Satoru Suwa, Kazuteru Fujimoto, Yasuharu Nakama, Takashi Morita, Wataru Shimizu, Yoshihiko Saito, Atsushi Hirohata, Yasuhiro Morita, Teruo Inoue, Asunori Okamura, Masaaki Uematsu, Kazuhito Hirta, Kengo Tanabe, Yoshisato Shibata, Mafumi Owa, Tetsuji Miura
Journal: Journal of the American College of Cardiology (2016)
AB1166 Henoch-schoenlein purpura: Clinical study of 340 patients
Authors: J. Rueda-Gotor, V. Calvo-Río, J. Loricera, F. Ortiz-Sanjuán, D. Lamuño, L. Martín, M.A. González-Lόpez, H. Fernández-Llaca, M.C. González-Vela, M. Arias, C. Mata-Arnáiz, E. Peirό, M.A. González-Gay, R. Blanco
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Most Henoch-Schonlein Purpura (HSP) studies came from selected series and used different classification criteria <h3>Objectives</h3> Our aim was to assess the epidemiological, clinical features, treatment and prognosis in an unselected and well-defined seriesof HSP. <h3>Methods</h3> Retrospective study of a wide series of patients from two hospitals diagnosed as having HSP. Patients with vasculitis secondary to connective-tissue diseases, malignancies, severe infections, primary systemic necrotizing vasculitides, and those with other well-defined clinical conditions such as hipersensitivity vasculitis and essential myxed cryoglobulinemia were excluded from this analysis. Patients were classified as having HSP according to the criteria proposed by Michel et al [1]. <h3>Results</h3> According to the above mentioned criteria 340 patients (194 men/146 women), with a mean ±SD age of 18.7±22.6 years (range; 1 to 87) were classified as having HSP. Most of the patients (256) were young people (subset established if the disease occurred in individuals younger than 20 years) (6.9±3.1 years) and 84 were adults (54.6±17.5 years). Precipitating events were found in 41.2% of patients. A history of drug intake before the onset of vasculitis was found in 19.7% and a previous upper respiratory tract infection in 35.3%. At disease onset, the most common manifestations were skin lesions (62%), abdominal pain (19.5%), joint symptoms (11%) and fever (8.8%). When the HSP disease was diagnosed the most frequent manifestations were cutaneous (99.7%), gastrointestinal (65.6%), joint (63%), and renal involvement (41.5%). The main laboratory data were elevated ESR (31.9%), leukocytosis (32.6%), anemia (8.5%), positive rheumatoid factor (6 of 139 cases tested), positive ANA (17 of 148), C3 and/or C4 decreased (14 of 285), negative cryoglobulins (60 of 78) and negative ANCAs (35 of 35). The more frequent treatments were: NSADs (11.2%), corticosteroids (33.5%), and cytotoxic agents (5.3%) (azathioprine in 6 cases, cyclophosphamide in 6 and methotrexate in 1). After a mean follow-up of 32.9±51.2 (median, 12) months, relapses were observed in 28.5% of patients, complete recovery in 84.1%, persisted mild renal insufficiency in 4 cases and mild hematuria in 26 cases. <h3>Conclusions</h3> HSP as defined by the criteria proposed by Michel et al, represents a relatively benign syndrome that affects predominantly to young population. <h3>References</h3> Michel BA, Hunder GG, Bloch DA, Calabrese LH. Hypersensitivity vasculitis and Henoch-Schönlein purpura: a comparison between the 2 disorders. J Rheumatol. 1992 May;19(5):721-8. <h3>Disclosure of Interest</h3> None Declared