tbuckley/cabot-search huggingface dataset:
SAT0141 Vitamin D Deficiency is A Risk Factor for Carotid Atherosclerosis in Women with Rheumatoid Arthritis: Table 1.
Authors: E.-K. Park, S.-G. Lee, G.-T. Kim, J.-H. Lee, S.-H. Baek, J.-W. Lee
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Although there is increasing evidence that low serum 25-hydroxy vitamin D (25-OHD) is associated with a higher frequency of cardiovascular diseases (CVDs) in the general population, little attention has been given to the relationship between vitamin D deficiency and atherosclerosis in patients with rheumatoid arthritis (RA). <h3>Objectives</h3> The present study aimed to investigate whether vitamin D deficiency is a potential risk factor for carotid atherosclerosis in patients with (RA). <h3>Methods</h3> With a cross-sectional design, we recruited 60 consecutive female RA patients without previous CVDs between September 2013 and December 2013 at the outpatient rheumatology clinic of a tertiary referral centre in South Korea. Carotid intima media thickness (CIMT) was measured using high-resolution ultrasonography (Philips HD15, Bothwell, WA, USA) and QLAB9s IMT-quantification software plug-in (Philips Healthcare, DA Best, The Netherlands) was used to enhance the consistency and reliability of CIMT measurement. Serum 25-OHD levels were assessed by radioimmunoassay. Stepwise multivariable linear regression models were used to evaluate the association between serum 25-OHD levels and CIMT. <h3>Results</h3> Mean (SD) age and disease duration were 56.0 (11.2) and 5.9 (4.9) years, respectively. Median 25-OHD levels (IQR) was 14.0 (11.0-20.7) ng/mL and 74% of patients had vitamin D deficiency (&lt;20ng/mL). Mean (SD) CIMT was 0.58 (0.08) mm and 24 (48%) RA patients had abnormal CIMT (≥6 mm). Compared to RA patients with normal CIMT (&lt;6mm), those with abnormal CIMT (≥6 mm) were older and had longer disease duration, lower serum 25-OHD levels and high density lipoprotein (HDL) and higher disease activity score (DAS) 28 and Korean version of health assessment questionnaire (K-HAQ) score. In univariable analyses, lower serum 25-OHD levels and HDL, older age, and higher DAS28 and K-HAQ scores were associated with increased CIMT (Table 1), while disease duration, blood pressure, triglyceride, low density lipoprotein and Homeostatic model assessment - insulin resistance showed no association with CIMT. Serum 25-OHD levels remains statistically significant in multivariable regression models after adjusting confounding factors (Table 1). <h3>Conclusions</h3> Our data suggests that vitamin D deficiency is a risk factor for subclinical atherosclerosis in female patients with RA. Larger prospective studies are needed to confirm our observation. <h3>Acknowledgements</h3> None <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.1510
The Effects of Physical Therapy on Cerebral Palsy
Authors: F B Palmer, B K Shapiro, R C Wachtel, M C Allen, J E Hiller, S E Harryman, B S Mosher, C L Meinert, A J Capute
Journal: New England Journal of Medicine (1988)

Legislatively mandated programs for early intervention on behalf of handicapped infants often stipulate the inclusion of physical therapy as a major component of treatment for cerebral palsy. To evaluate the effects of physical therapy, we randomly assigned 48 infants (12 to 19 months of age) with mild to severe spastic diplegia to receive either 12 months of physical therapy (Group A) or 6 months of physical therapy preceded by 6 months of infant stimulation (Group B). The infant-stimulation program included motor, sensory, language, and cognitive activities of increasing complexity. Masked outcome assessment was performed after both 6 and 12 months of therapy to evaluate motor quotient, motor ability, and mental quotient. After six months, the infants in Group A had a lower mean motor quotient than those in Group B (49.1 vs. 58.1, P = 0.02) and were less likely to walk (12 vs. 35 percent, P = 0.07). These differences persisted after 12 months of therapy (47.9 vs. 63.3, P less than 0.01, and 36 vs. 73 percent, P = 0.01, respectively). We noted no significant differences between the groups in the incidence of contractures or the need for bracing or orthopedic surgery. Group A also had a lower mean mental quotient than Group B after six months of therapy (65.6 vs. 75.5, P = 0.05). The routine use of physical therapy in infants with spastic diplegia offered no short-term advantage over infant stimulation. Because of the limited scope of the trial, our conclusions favoring infant stimulation are preliminary. The results suggest that further study of the effects of both physical therapy and infant stimulation is indicated.
Abstract 4732: Cis-expression QTL analysis of established risk variants for colorectal cancer
Authors: Lenora W.M Loo, Iona Cheng, Maarit Tiirikainen, Annette Lum-Jones, Ann Seifried, Lucas M. Dunklee, Steve Gallinger, Stephen N. Thibodeau, Graham Casey, Loic Le Marchand
Journal: Cancer Research (2011)

Abstract Introduction: Genome-wide association studies of colorectal cancer have identified 18 risk variants that explain ∼25% of the hereditable risk of colorectal cancer. These variants are located in intronic, inter-genic or gene-desert regions. To uncover whether some of these variants may have a regulatory function on the expression of near-by genes, we conducted expression quantitative trait loci (eQTL) analyses of these 18 regions. Methods: DNA and RNA were extracted from 40 fresh-frozen paired colon tumor (microsatellite stable and CpG island methylator phenotype-negative) and adjacent normal tissue samples collected by the Colon Cancer Family Registry. Whole genome gene expression assessment was performed in the tumors and adjacent normal tissue with the Affymetrix GeneChip Human Exon 1.0 ST array. Genome-wide SNP data was generated with DNA extracted from the same normal tissue samples using the Affymetrix Genome-Wide Human SNP 6.0 array. Partek software was used to compare the expression levels by genotype for each gene spanning a 2 Mb region up- and down- stream of each of the 18 established risk variants for colorectal cancer. Results: Three of the 18 risk variants (rs10795668, rs4444235, rs9929218) demonstrated significant cis-eQTLs (FDR q-values&amp;lt;0.05). Specifically, differential gene expression was observed in genes neighboring rs10795668 at 10p14 (ATP5C1, q=0.024), rs4444235 at 14q22.2 (DLGAP5, q=0.041), and rs9929218 at 16q22.1 (NOL3, q=0.017; DDX28, q=0.046). In particular, DLGAP5 (discs, large (Drosophila) homolog-associated protein 5) at 14q22.2 and NOL3 (nucleolar protein 3) at 16q22 are interesting candidate genes for colorectal cancer. DLGAP5 encodes for a microtubule-associated protein and functions as a mitotic regulator that has been reported to be overexpressed in colon tumors. NOL3 (nucleolar protein 3) encodes for an anti-apoptotic protein that down-regulates the expression of caspase 2 and 8, and p53. NOL3 also appears to be overexpressed in colorectal tumors. Conclusion: In summary, these findings suggest putative functional activities for three established colorectal risk variants and highlight potentially interesting candidate genes for this malignancy. If confirmed, these results may help explain the total genetic risk of the disease, as well as further our understanding of the underlying biology of colorectal cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4732. doi:10.1158/1538-7445.AM2011-4732
Evaluation of Tolserol in the Treatment of Children with Cerebral Palsy
Authors: Eric Denhoff, Raymond H. Holden, Caroll M. Silver
Journal: New England Journal of Medicine (1949)

THE basic concept of the treatment of cerebral palsy is re-education in the form of physical and occupational therapy. This treatment might be enhanced if a drug were found that would favorably influence the disabilities peculiar to this syndrome. This drug should have a relaxing effect on spastic and athetoid states and should facilitate the correction of resulting deformities. Since such a therapeutic program cannot be successful without the co-operation of the patient, the drug would be even more valuable if it were to alter favorably the child's attitude and behavior.Tolserol (3 o-toloxy 1,2-propanediol) has been reported to effect . . .
Abstract LB-119: Production of functionally active glycoproteins and phosphoproteins based on a novel human in vitro expression system
Authors: Greg Kilmer, Krishna Vattem, Eric Hommema, Ken Maas, Atul Deshpande, Brian L. Webb
Journal: Cancer Research (2010)

Abstract One of the key features of the human proteome is the abundance of post-translation modification (PTM), with more than 50% of proteins being glycosylated and more than 30% of all the proteins regulated by kinase activities. The alteration of protein glycosylation and other PTMs such as phosphorylation in cancer cells is closely related to the onset and progression of cancer and other disease states. In collaboration with RIKEN, we have developed a novel human in vitro translation (also known as cell-free expression) system, that can produce biologically active glycoproteins such as human chorionogonadotrophic hormone as well as active protein kinases such as Akt, MAPK and Aurora A kinase. A highly efficient PCR based approach will also be discussed that eliminates the need for cloning of genes into the expression vector and provides an easier expression of genes present in any other vector. In addition we demonstrate that our in vitro human expression, unlike the only other commercially existing mammalian expression system based on rabbit reticulocytes, can sustain expression of proteins for over 6 hours without the need to remove of inhibitory by products and is compatible with fluorimetric and colorimetric assays. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-119.
Polypoid and non-pigmented small-bowel melanoma in capsule endoscopy is common
Authors: E. Prakoso, W. Selby
Journal: Endoscopy (2010)

We read with interest the case report by Urbain et al. "Small-bowel metastasis of malignant melanoma: video capsule endoscopy appearance" [1]. The typical melanotic lesion reported by the authors, "solar eclipse" appearance with the central coal-black lesion encircled by whitish intestinal villi [1], certainly enriches the variety of small-bowel melanoma appearances seen in capsule endoscopy.
FRI0484 One year results of treatment efficacy on bone microarchitecture and joint space width in early inflammatory arthritis measured with hr-pqct
Authors: C. Barnabe, M. Kan, L. Martin, S. K. Boyd
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> New sensitive imaging techniques may detect longitudinal improvements in bone quality with treatment in early inflammatory arthritis. <h3>Objectives</h3> Quantify measures of bone damage in the first year of rheumatoid arthritis (RA) and undifferentiated arthritis (UA) by high-resolution peripheral quantitative computed tomography (HR-pQCT, Scanco Medical AG, Switzerland), which provides bone microarchitecture resolution at 82 μm. <h3>Methods</h3> HR-pQCT images of the 2nd and 3rd MCP joints of subjects at diagnosis and controls were obtained, with repeat scans at 1 year. The manufacturer's standard evaluation protocol was applied to quantify measures of bone density and microarchitecture. Joint space width was measured using a custom analysis implemented for the HR-pQCT based on direct measurements from the high resolution image data. <h3>Results</h3> Twenty-one subjects with inflammatory disease (RA=17, UA=4) and three controls have been scanned. These subjects are 71% female, mean 45 years of age, mean symptom duration at diagnosis 6.5 months, BMI 28.6, 41% are RF positive and 67% anti-CCP positive. The mean reduction at 1 year for clinical parameters was: tender joint counts 11.3 (SD 8.3)/28, swollen joint count 9.7 (SD 6.9)/28, HAQ 0.68 (SD 0.82) and DAS28 2.93 (SD 2.13). <h3>Conclusions</h3> Treatment initiation in early inflammatory arthritis results in stabilization of bone density and no joint space narrowing was observed. Bone density was lower in patients with inflammatory disease and did not improve with treatment despite improvement in clinical disease activity measures. <h3>Disclosure of Interest</h3> None Declared
MPs call for improvement on cost, duplication, and delays in ambulance services
Authors: A. O'Dowd
Journal: BMJ (2011)

England’s ambulance service inefficiencies should be tackled to ensure patients are not kept waiting outside emergency departments and costs are evened out across the country, say MPs.

In a report published on 15 September, MPs on the parliamentary public accounts committee called for improvements to the service despite its existing good reputation with the general public.

The report, Transforming NHS Ambulance Services, says there is much to be praised about the “valuable” service provided by the 11 regional ambulance services across England, which, collectively, handled 7.9 million emergency calls in 2009-10.

However, there were several inefficiencies around the service that should be addressed, said the MPs, such as the fact that some ambulance …
P66 Low pre-treatment plasma HBsAg level and its decline during add-on interferon treatment predict response to combined lamivudine/interferon therapy in tolerant children with infancy-acquired chronic hepatitis B
Authors: I. Carey, M. Horner, M. Bruce, S. Bansal, D. Vergani, G. Mieli-Vergani
Journal: Gut (2011)

<h3>Introduction</h3> Changes in HBsAg plasma levels during antiviral therapy with pegylated interferon (IFN) predict response in adults with chronic hepatitis B (CH-B). Whether pre-treatment HBsAg plasma levels correlate with liver relaxed circular (RC) HBV DNA and covalently closed circular (ccc) DNA is controversial. To date, no information is available on HBsAg plasma level behaviour and response to treatment prediction in tolerant patients with infancy-acquired CH-B treated with lamivudine (LAM) and IFN. <h3>Aim</h3> To investigate whether HBsAg plasma levels predict response to LAM + IFN treatment in tolerant children with infancy-acquired CH-B, and to determine their association with plasma HBV DNA levels during treatment and with pre-treatment liver RC HBV DNA and cccDNA. <h3>Method</h3> <b>Patients:</b> 23 children (8 males, median age 10.2 yrs) with infancy-acquired CH-B (all HBeAg positive), treated for 52 weeks [lead-in LAM (3 mg/kg/d) for 9 weeks; LAM plus IFN-α (5 MU/m<sup>2</sup> TIW) from week 9 for 44 weeks], were divided according to treatment response: 5 responders (R = anti-HBs seroconversion) and 18 non-responders (NR). <b>Methods:</b> Plasma HBsAg and HBV DNA levels were measured before (treatment week 0, TW0), during (TW9, TW28, TW52) and after (follow-up week, FUW24) therapy by Abbott ARCHTECT_ assay and real-time TaqMan PCR [both log<sub>10</sub> IU/ml]. Baseline liver RC HBV DNA and cccDNA was quantified by real-time TaqMan PCR [copies/ng genomic DNA]. Results are presented as median. <h3>Results</h3> Baseline HBsAg levels were lower in R than NR (4.36 vs 4.74, p=0.02), but similar in R and NR at the end of LAM lead-in therapy (TW9) (4.34 vs 4.66, p=0.1). During IFN add-on therapy, at TW28 (2.34 vs 4.33) and TW52 (0 vs 4.08) levels were markedly lower in R than NR, the difference persisting at FUW24 (0 vs 4.51) (p&lt;0.01 for all). Plasma HBV DNA levels were similar at baseline in R and NR (8.94 vs 8.98), but decreased significantly in R compared to NR at TW9 (4.91 vs 5.48), TW28 (3.42 vs 4.39), TW52 (1.57 vs 4.07) and FUW24 (0.27 vs 7.75) (p&lt;0.01 for all). There was a strong positive correlation between plasma HBsAg and HBV DNA levels at TW28 (r=0.6, p&lt;0.01) and TW52 (r=0.64, p&lt;0.01). Baseline liver RC HBV DNA (43 800 vs 52 300 copies/ng genomic DNA) and cccDNA (41 vs 49 copies/ng genomic DNA) were similar in R and NR, with no correlation between liver RC HBV DNA or cccDNA and baseline HBsAg. <h3>Conclusion</h3> Lower baseline HBsAg plasma levels and a sharp decrease of plasma HBV DNA levels at TW9 (LAM lead-in) followed by declining plasma HBsAg levels from TW28 (IFN add-on) heralds HBsAg clearance and response to treatment in tolerant children with CH-B.
Abstract 4474: Cellular effects of fluorescent quinolinium drugs on normal lymphoblast and A431 carcinoma cells
Authors: Christian Velez, Denisse Molina, Carlos Rosado, Osvaldo Cox, Beatriz Zayas
Journal: Cancer Research (2011)

Abstract This study aims to evaluate the cellular effects of amino benzazolo [3,2- a] quinolinium drugs on normal lymphoblast cells and compare them to effects on A431 epitheloid carcinoma cells. The drugs under study are amino containing heterocyclic compounds possessing a positive charge that could facilitate their interaction with cell organelles specially mitochondria. The fluorescent properties could also facilitate observation of their cellular binding location. These compounds have also been demonstrated to have mutagenic and DNA binding capacity on A431 cancer cells. A431 and TK6 cells in culture where exposed for 48 hours to determine the IC50 dose, mitochondrial membrane permeability, ROS generation and fluorescent microscopy analysis of intracellular binding. IC50 was determined by trypan blue exclusion and mitochondrial membrane permeability applying the Nucleo counter 3000, JC-1 assay. For ROS determination the fluorescent dye 2,7-dichlorofluorescein diacetate (DCFH-DA) was applied. Determination of intracellular binding was performed applying confocal microscopy. In the TK6 normal lymphoblast cells the observed IC50 concentrations were as follow: ABQ38=310µM, and ABQ95=270µM. On A431 IC50s were as follows: ABQ38 = 32 µM and ABQ95 = 36 µM. Overall, normal TK6 demonstrated higher tolerance to the tested ABQS than A 431. Preliminary results on ROS generation indicated a stronger ROS release on A431 than on TK6 lymphoblast. Analysis of the effects of ABQS on the mitochondrial permeability where also consistent with the cytotoxicity observed, demonstrating higher levels of membrane permeability on treated tumor cells than on normal. Microscopy images of ABQS treated cells revealed location of these fluorescent compounds in organelles such as mitochondria and DNA. DNA fragmentation analyses are being implemented. This study presents preliminary evidence of an overall higher tolerance of normal TK6 lymphoblast to the tested fluorescent drugs in comparison to treated A431. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4474. doi:10.1158/1538-7445.AM2011-4474
OP18 * IDENTIFICATION AND CHARACTERISATION OF MICRORNAS INVOLVED IN GLIOBLASTOMA CELL PROLIFERATION AND SURVIVAL
Authors: M. Boissinot, J. Hayes, M. Adams, J. Higgins, D. Tomlinson, S. E. Lawler, S. Short
Journal: Neuro-Oncology (2014)

INTRODUCTION: There is a pressing need for new therapeutic approaches for the treatment of glioblastoma (GBM). MicroRNAs(miRs)are single-stranded non-coding RNAs, 22-24nt in length, that function by reducing translation or causing degradation of target mRNAs. MiRs have been shown to play roles in multiple hallmark characteristics of GBM,suggesting that miRs and their associated pathways may be of therapeutic importance. METHOD: The miRIDIAN mimic library (Dharmacon) that encompasses all human miRs annotated in miRBase v16.0 was used with a high-throughput imaging platform (Operetta) to identify miRs with potent effects on GBM cell proliferation and survival. Screens were performed in duplicate on U251 (adult) and KNS42 (paediatric) GBM cell lines. Cell number was assessed 72h post-transfection and expressed as z-scores of the nuclei count. MiRs were considered significant candidates if their mean z-score was below zero, indicating a decrease in cell number, and if it differed from that of the negative control by at least two standard deviations.Validation included RTqPCR, imaging and flow cytometry based assays. RESULTS: For each cell line, the functional screen resulted in approximately 100 candidates, of which 70% were in both cell lines. Seven miRs were validated in a panel of 4 adult and 2 paediatric GBM cell lines.Mir-X was identified as the most potent, robust candidate involved in GBM cell death across all cell lines. This miR consistently caused a G2M cell cycle arrest followed by apoptosis. CONCLUSION: We have identified a novel potent pro-apoptotic miR in GBM that may be relevant in therapy. Work is ongoing to characterise the targets and mechanisms responsible for this effect and test for efficacy in vivo.
Widespread implementation of EUCAST breakpoints for antibacterial susceptibility testing in Europe
Authors: D Brown, R Cantón, L Dubreuil, S Gatermann, C Giske, A MacGowan, L Martínez-Martínez, J Mouton, R Skov, M Steinbakk, C Walton, O Heuer, M J Struelens, L Diaz Högberg, G Kahlmeter
Journal: Eurosurveillance (2015)

The European Committee on Antimicrobial Susceptibility Testing (EUCAST) was established to harmonise clinical antimicrobial breakpoints and to define breakpoints for new agents in Europe. Data from the European Antimicrobial Resistance Surveillance Network (EARS-Net) external quality assessment (EQA) exercises from 2009 to 2012, from the United Kingdom External Quality Assessment Scheme (UK NEQAS) from November 2009 to March 2013 and data collected by EUCAST through a questionnaire in the first quarter of 2013 were analysed to investigate implementation of EUCAST guidelines in Europe. A rapid change to use of EUCAST breakpoints was observed over time. Figures for implementation of EUCAST breakpoints at the end of the studied period were 61.2% from EARSNet data and 73.2% from UK NEQAS data. Responses to the EUCAST questionnaire indicated that EUCAST breakpoints were used by over 50% of laboratories in 18 countries, by 10 to 50% of laboratories in eight countries and by less than 10% in seven countries. The EUCAST disk diffusion method was used by more than 50% of laboratories in 12 countries, by 10 to 50% of laboratories in ten countries and by less than 10% in eleven countries. EUCAST guidelines implementation is essential to ensure consistent clinical reporting of antimicrobial susceptibility results and antimicrobial resistance surveillance.
Images of EUS and pathological features in cases with carcinoma in situ of the pancreas
Authors: Hanada Keiji
Journal: Endoscopy (2011)

Backgrounds: Pancreatic carcinoma is hard to diagnose in the early stage. In our doctor communities around Onomichi City, the screening consensus including EUS between high volume centers and regional doctors has been established in the early diagnosis of small pancreatic carcinoma. In this study, we report the images of EUS and pathological features in cases with carcinoma in situ of the pancreas.
LONG-TERM EFFECTS OF EPIRUBICIN ON INCIDENCE OF HEART FAILURE IN WOMEN WITH BREAST CANCER: DATA FROM A RANDOMIZED CLINICAL TRIAL
Authors: Ann Bøcher Secher Banke, Emil Fosbol, Jacob Møller, Gunnar Gislason, Mads Andersen, Mogens Bernsdorf, Maj-Britt Jensen, Morten Schou, Bent Ejlertsen
Journal: Journal of the American College of Cardiology (2016)