tbuckley/cabot-search huggingface dataset:
AB0667 Hlab27 Associated Uveitis Trends and Patterns of A Multiethnicity Cohort: Need for Combined Rheumatology and Eye Services?
Authors: A. Pradeep, P. Kumar, A. Moorthy
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Uveitis is associated with systemic autoimmune disease and often present to Eye Department as an emergency. Uveitis can be a first presenting symptom of systemic autoimmune disease and often not followed up in Rheumatology services. Approximately 40% of patients with acute anterior uveitis (AAU) have undiagnosed spondyloarthritis (1). Previous studies in UK by Hamilton et al showing delay in diagnosing spondyloarthropathy (SpA) up to 8 years (2). Delay in referral of HLA B27 positive Uveitis by primary care and secondary care physicians to Rheumatology may be a reason for the delayed diagnosis (3,4).This can be better avoided if inter-speciality referral and pathways are established. To enable the best effective care delivered for Uveitis patients,we attempted to review the current trends of our own cohort of patients with HLAB27 Associated Uveitis <h3>Objectives</h3> - To review the cohort of HLA B27 positive uveitis in a busy tertiary care hospital serving multi ethnic patients. - To observe the pattern and association of systemic autoimmune disease particularly spondyloarthropathy. - To see how many cases been referred to Rheumatology services and how best they are managed with systemic immunosuppressive therapy. <h3>Methods</h3> The methodology includes retrospective analysis of data captured from the Uveitis Data Base maintained by ophthalmology department. The consecutive new cases of HLAB27 uveitis were identified for three-year period from 2011 to 2014. The clinical records, ophthalmology examination records, demographic details, ethnicity were reviewed. The type of uveitis, presenting symptoms and systemic associations were explored. Modes of treatment, complications and pattern of referral and input from other specialties were assessed as well treatment plans were collected. The data were captured by using standardized proforma. The captured data was analysed using excel spreadsheet. <h3>Results</h3> Total of 624 Uveitis patients 73 cases (105 eyes) of HLAB27 positive uveitis (11.7%) were identified.The most common systemic association in our cohort was Ankylosing Spondylitis (31.8%). 34%of the affected eyes did not develop anycomplications.88% had anterior uveitis with 38% showing chronicity.79% of the patients had better than 0.3LogMar acuity in the affected eye on presentation. <h3>Conclusions</h3> The data collected emphasizes the higher incidence of Ankylosing Spondylitis in patients, who presents with uveitis associated with positive HLA B27. Our result reiterates the need for establishing combined clinics to avoid delay in referrals enhancing patient management and experience. <h3>Disclosure of Interest</h3> None declared
THU0009 adiponectin-AdipoR1-STAT3 Dependent Pathway Is A Critical Regulator of Th17 Cell Differentiation in Collagen-Induced Arthritis
Authors: M. Zhang
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> We previously showed that adiponectin (AD) acts to promote Th17 cell differentiation, suggesting that AD plays an important role in mediating joint inflammation and damage in collagen-induced arthritis (CIA). <h3>Objectives</h3> The aim of this study was to investigate whether STAT3 expression is regulated by AD, and if so, whether knockdown AdipoR1 could inhibit STAT3 and Th17 expression and prevent disease progression in CIA. <h3>Methods</h3> Expression of Th17, AD and STAT3 in joint tissue of CIA was detected by real-time PCR and flow cytometry. STAT3 promoter activity was analyzed using a Dual-Luciferase Reporter Assay. The effect of AD on STAT3 expression in <i>vitro</i> and <i>vivo</i> was assessed using histological analysis, real-time PCR and flow cytometry. <h3>Results</h3> Expression of STAT3 was up-regulated and positively correlated with highly expressed AD in joint tissue of CIA. Moreover, AD treatment markedly enhanced STAT3 expression both in <i>vitro</i> and <i>vivo</i>. Moreover, we found that the anti-Th17 differentiation effect of STAT3 inhibitor can be stimulated by addition of exogenous AD partly. Mechanistically, we showed AD activated STAT3 transcription by binding to the promoter region of the STAT3 gene. Further, using the CIA model, we showed that treatment with the AdipoR1 RNA interference led to reduction of STAT3 levels, decrease of Th17 response, and attenuation of inflammation and disease progression in <i>vivo</i>. <h3>Conclusions</h3> Our results not only reveal a new role of AD in promoting Th17 development via upregulation of STAT3 expression. Our study also suggests that the pro-Th17 cell differentiation effect of AD is mediated through AdipoR1. <h3>Disclosure of Interest</h3> None declared
CMR First-Pass Perfusion for Suspected Inducible Myocardial Ischemia
Authors: Robert C Hendel, Matthias G Friedrich, Jeanette Schulz-Menger, Claudia Zemmrich, Frank Bengel, Daniel S Berman, Paolo G Camici, Scott D Flamm, Dominique Le Guludec, Raymond Kim, Massimo Lombardi, John Mahmarian, Udo Sechtem, Eike Nagel
Journal: JACC. Cardiovascular imaging (2016)
Ca2+/NFAT Signaling Regulates the Expression CD38 and ZAP70 in Murine B Cells and Controls B1a Cell Homeostasis
Authors: Melanie Märklin, Stefanie Bugl, Marina Bechtel, Alexandra Poljak, Hans-Georg Kopp, Lothar Kanz, Anjana Rao, Stefan Wirths, Martin R Müller
Journal: Blood (2011)
Clinical Trial of Therapeutic Blockade of CTLA4 with Ipilimumab in Patients with Relapse of Malignancy Following Allogeneic Hematopoietic Transplantation.
Authors: Asad Bashey, Bridget Medina, Sue Corringham, Mildred Pasek, Ewa Carrier, Linda Vrooman, Howard Streicher, Israel Lowy, Scott R. Solomon, Lawrence E. Morris, Kent Holland, James R. Mason, Robert J. Soiffer, Edward D. Ball
Journal: Blood (2007)
The Efficacy of R-ICE Therapy as a Salvage Treatment for Relapse and Refractory Diffuse Large B-Cell Lymphoma.
Authors: Makoto Kodaira, Masahiro Yokoyama, Hiroaki Asai, Shuhei Yamada, Kyoko Ueda, Tomohiro Myojo, Tomoko Nishimura, Yuko Mishima, Sakura Sakajiri, Kengo Takeuchi, Takashi Saotome, Yasuhito Terui, Shunji Takahashi, Kiyohiko Hatake
Journal: Blood (2007)
Thymic 18F-FDG PET/CT Findings in Pediatric Malignant Lymphoma after Chemotherapy: Differentiation of Long-Lasting Thymic Hyperplasia from Lymphoma Recurrence
Authors: Toshihiro Fujiki, Hideaki Maeba, Raita Araki, Yasuhiro Ikawa, Rie Kuroda, Shintaro Mase, Masaki Fukuda, Shoichi Koizumi, Akihiro Yachie, Ryosei Nishimura
Journal: Blood (2014)
South Brazilian Chronic Myeloid Leukemia (CML) Profile Patients: How We Treat and Our Results
Authors: Laura Fogliatto, Marcelo Capra, Mariza Schaan, Mario Sergio Fernandes, Waldir Pereira, Dalnei Pereira, Virginia Coser, Tania Hellwig, Gilca Nachtigal, Aletea Zago, Marco Schilling, Denise Pereira, Moema Nene, Sabrina Wisenstein, Roque Lorandi, Cheila Eickhof, Victor Hugo Lenz, Adriana Zardo, Adriana Wink, Luciana Tagliari, Inez Guterrez, Lisandra Dellacosta, Tito Vanelli, Ingrid Mito, Lucia Silla
Journal: Blood (2008)
Transcriptional Selectivity of Epigenetic Therapy in Cancer
Authors: Takahiro Sato, Matteo Cesaroni, Woonbok Chung, Shoghag Panjarian, Anthony Tran, Jozef Madzo, Yasuyuki Okamoto, Hanghang Zhang, Xiaowei Chen, Jaroslav Jelinek, Jean-Pierre J. Issa
Journal: Cancer Research (2016)

Abstract A central challenge in the development of epigenetic cancer therapy is the ability to direct selectivity in modulating gene expression for disease-selective efficacy. To address this issue, we characterized by RNA-seq, DNA methylation, and ChIP-seq analyses the epigenetic response of a set of colon, breast, and leukemia cancer cell lines to small-molecule inhibitors against DNA methyltransferases (DAC), histone deacetylases (Depsi), histone demethylases (KDM1A inhibitor S2101), and histone methylases (EHMT2 inhibitor UNC0638 and EZH2 inhibitor GSK343). We also characterized the effects of DAC as combined with the other compounds. Averaged over the cancer cell models used, we found that DAC affected 8.6% of the transcriptome and that 95.4% of the genes affected were upregulated. DAC preferentially regulated genes that were silenced in cancer and that were methylated at their promoters. In contrast, Depsi affected the expression of 30.4% of the transcriptome but showed little selectivity for gene upregulation or silenced genes. S2101, UNC0638, and GSK343 affected only 2% of the transcriptome, with UNC0638 and GSK343 preferentially targeting genes marked with H3K9me2 or H3K27me3, respectively. When combined with histone methylase inhibitors, the extent of gene upregulation by DAC was extended while still maintaining selectivity for DNA-methylated genes and silenced genes. However, the genes upregulated by combination treatment exhibited limited overlap, indicating the possibility of targeting distinct sets of genes based on different epigenetic therapy combinations. Overall, our results demonstrated that DNA methyltransferase inhibitors preferentially target cancer-relevant genes and can be combined with inhibitors targeting histone methylation for synergistic effects while still maintaining selectivity. Cancer Res; 77(2); 470–81. ©2016 AACR.
The Type of Upfront Induction Therapy for Newly Diagnosed Multiple Myeloma Patients Has No Significant Impact on Clinical Outcomes after Autologous Hematopoietic Stem Cell Transplantation.
Authors: Stephen S. Chung, Thomas R. Spitzer, David T. Ting, Christine Dube, Andrew J. Yee, Karen Ballen, Steven L. McAfee, Eyal Attar, Bimalangshu R. Dey
Journal: Blood (2007)
Dose and Timing Limitations in the Use of Bone Marrow-Derived Mesenchymal Stem Cells for the Treatment of Experimental Arthritis.
Authors: Evangelia Yannaki, Anastasia Papadopoulou, Minas Yiangou, Evangelia Athanasiou, Argyrw Paraskeva, Ioannis Batsis, Eleftheria Tsolaki, Angeliki Xagorari, Achilles Anagnostopoulos, Athanasios Fassas
Journal: Blood (2008)
AB0163 No Association between The Percentage of Memory or Transitional B Regs and Disease-Related Autoantibodies in Systemic Sclerosis
Authors: A. Mavropoulos, C. Liaskos, T. Simopoulou, A. Varna, C.G. Katsiari, D.P. Bogdanos, L.I. Sakkas
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Recently, we found that Breg cells are numerically decreased and functionally impaired in patients with systemic sclerosis (SSc)<sup>1</sup> A subsequent study confirmed these data and reported a negative correlation between Breg levels and the titres of anti-topo I and anti-centromere antibodies (ab) in patients with SSc<sup>2</sup>. <h3>Objectives</h3> To assess the relation between memory or transitional Bregs with SSc-specific anti-topo I, anti-centromere (CEN) and anti-RNA pol I abs in our cohort of SSc patients. <h3>Methods</h3> Fifty patients with Ssc were tested for disease-specific abs (Topo I, CENP, RNA pol I) using line blot analysis (EUROIMMUN), and for memory [CD19(pos)CD27(pos)CD24(high)] and transitional [CD19(pos)CD24(high)CD38(high)] Bregs using flow cytometry. <h3>Results</h3> Anti-CEN, anti-Scl-70 and RNA pol I abs were present in 12 (24%), 23 (46%) and 7 (14%) patients with SSc, respectively. The % of memory and transitional B regs was comparable between anti-CEN ab-positive and -negative patients (1.56±1.40 vs 1.74±1.01 and 0.78 ±0.73 vs 0.65±0.68 respectively, p&gt;0.05), and also beween anti-RNA ab-positive and -negative patients with SSc (1.96±1.26 vs 1.65±1.09 and 0.54±0.53 vs 0.7±0.72 respectively). While the % of memory Bregs did not differ between anti-topo I-positive and -negative patients (1.96±1.26 vs 1.65±1.09 and 0.54 ±0.53 vs 0.7±0.72 respectively), the % of transitional Bregs tended to be decreased in patients with anti-topo I-positive compared to anti-topo I ab-negative patients (0.49±0.46 vs 0.84±0.61 p=0.066). Levels of B regs (memory or transitional) did not negatively correlate with autoantibody titres by line blot analysis. <h3>Conclusions</h3> We failed to find an association between percentages of memory and transitional B regs with SSc-specific autoantibodies. <h3>References</h3> Mavropoulos A et al Arthritis Rheumatol 2016;68:494–504 Matsushita T et al Rheumatology 2016;55:263–7 <h3>Acknowledgement</h3> Financial support by ELKE, University of Thessaly <h3>Disclosure of Interest</h3> None declared
Antitumor therapeutic application of self-assembled RNAi-AuNP nanoconstructs: Combination of VEGF-RNAi and photothermal ablation
Authors: Sejin Son, Namho Kim, Dong Gil You, Hong Yeol Yoon, Ji Young Yhee, Kwangmeyung Kim, Ick Chan Kwon, Sun Hwa Kim
Journal: Theranostics (2016)

Nucleic acid-directed self-assembly provides an attractive method to fabricate prerequisite nanoscale structures for a wide range of technological applications due to the remarkable programmability of DNA/RNA molecules. In this study, exquisite RNAi-AuNP nanoconstructs with various geometries were developed by utilizing anti-VEGF siRNA molecules as RNAi-based therapeutics in addition to their role as building blocks for programmed self-assembly. In particular, the anti-VEGF siRNA-functionalized AuNP nanoconstructs can take additional advantage of gold-nanoclusters for photothermal cancer therapeutic agent. A noticeable technical aspect of self-assembled RNAi-AuNP nanoconstructs in this study is the precise conjugation and separation of designated numbers of therapeutic siRNA onto AuNP to develop highly sophisticated RNA-based building blocks capable of creating various geometries of RNAi-AuNP nano-assemblies. The therapeutic potential of RNAi-AuNP nanoconstructs was validated in vivo as well as in vitro by combining heat generation capability of AuNP and anti-angiogenesis mechanism of siRNA. This strategy of combining anti-VEGF mechanism for depleting angiogenesis process at initial tumor progression and complete ablation of residual tumors with photothermal activity of AuNP at later tumor stage showed effective tumor growth inhibition and tumor ablation with PC-3 tumor bearing mice.
Treatment Outcomes with Pomalidomide (POM) in Combination with Low-Dose Dexamethasone (LoDex) in Patients with Relapsed and Refractory Multiple Myeloma (RRMM) and Del(17p13) and/or t(4;14)(p16;q32) Cytogenetic Abnormalities Who Have Received Prior Therapy with Lenalidomide (LEN) and Bortezomib (BORT)
Authors: Paul G. Richardson, Andrzej Jakubowiak, Nizar J. Bahlis, David S. Siegel, Christine I. Chen, Min Chen, Mohamed Zaki, Gail Larkins, Kenneth C. Anderson
Journal: Blood (2012)
RADAR Update on Thalidomide (Thal)- and Lenalidomide (Len)-Associated Venous Thromboembolism (VTE): Safety Concerns Persist for Multiple Myeloma (MM) Despite FDA Approvals in This Setting.
Authors: Charles L. Bennett, Zehra Hussain, Mark Courtney, Paul Yarnold, Dennis Raisch, June M. McKoy
Journal: Blood (2006)
Phase I Trial of Escalated Doses of Targeted Marrow Radiation Delivered by Tomotherapy Combined with Etoposide and Cyclophosphamide; An Allogeneic HCT Preparative Regimen for Patients with Advanced Leukemia
Authors: Anthony Selwyn Stein, Jeffrey Wong, Joycelynne Palmer, Margaret O'Donnell, David S. Snyder, Ni-Chun Tsai, Pablo Parker, Len Farol, Ricardo Spielberger, Firoozeh Sahebi, Neil Kogut, Joyce Murata-Collins, David Senitzer, Stephen J. Forman
Journal: Blood (2012)
Metabolomic biomarkers predictive of early structural lung disease in cystic fibrosis
Authors: Charles R. Esther, Lidija Turkovic, Tim Rosenow, Marianne S. Muhlebach, Richard C. Boucher, Sarath Ranganathan, Stephen M. Stick
Journal: European Respiratory Journal (2016)

Neutrophilic airway inflammation plays a role in early structural lung disease in cystic fibrosis, but the mechanisms underlying this pathway are incompletely understood. Metabolites associated with neutrophilic inflammation were identified by discovery metabolomics on bronchoalveolar lavage fluid supernatant from 20 preschool children (2.9±1.3 years) with cystic fibrosis. Targeted mass-spectrometric detection of relevant metabolites was then applied to 34 children (3.5±1.5 years) enrolled in the Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST CF) who underwent chest computed tomography and bronchoalveolar lavage from two separate lobes during 42 visits. Relationships between metabolites and localised structural lung disease were assessed using multivariate analyses. Discovery metabolomics identified 93 metabolites associated with neutrophilic inflammation, including pathways involved in metabolism of adenyl purines, amino acids and small peptides, cellular energy and lipids. In targeted mass spectrometry, products of adenosine metabolism, protein catabolism and oxidative stress were associated with structural lung disease and predicted future bronchiectasis, and activities of enzymes associated with adenosine metabolism were elevated in the samples with early disease. Metabolomics analyses revealed metabolites and pathways altered with neutrophilic inflammation and destructive lung disease. These pathways can serve as biomarkers and potential therapeutic targets for early cystic fibrosis lung disease.
Homozygous Deletion of p16, p14 and p15 Is a Poor Prognostic Factor in Adult B-Lineage Acute Lymphoblastic Leukemia, Not in Childhood B-ALL: A Comparison through Deletion and Hypermethylation Study
Authors: Miyoung Kim, Seon-Hee Yim, Hai Rim Shin, Nam Sun Cho, Seong_Ho Kang, Ho Young Kim, Bo Ra Oh, Tae Young Kim, Hyun Jung Min, Cha Ja She, Hyo Seop Ahn, Hee Young Shin, Hyoung Jin Kang, Sung-Soo Yoon, Byoung Kook Kim, Kyu Sup Han, Han_Ik Cho, Dong Soon Lee
Journal: Blood (2008)
Non-Invasive Hemoglobin Estimation in Patients with β-Thalassemia Major
Authors: Murtadha K. Al-Khabori, Arwa Z. Al-Riyami, Mohammed Al-Huneini, Khalil Al Farsi, Abdulhakeem Al-Hashim, Nasser Al-Kemyani, Hussain M Mohsin, Shahina Daar
Journal: Blood (2012)
Posttransplant Immunotherapy with a GM-CSF-Based Tumor Vaccine (GVAX®) Following Autologous Stem Cell Transplant (ASCT) for Acute Myeloid Leukemia (AML).
Authors: Daniel J. DeAngelo, Edwin P. Alyea, Ivan M. Borrello, Hyam I. Levitsky, Wendy Stock, Dorie Sher, Lloyd E. Damon, Charles A. Linker, Amy Donnelly, Kristen M. Hege
Journal: Blood (2004)
Formation of the Functional Niche in Vitro by Mimicking the Pathophysiological Features of the Bone Marrow Microenvironment in Multiple Myeloma
Authors: Jana Jakubikova, Richard W.J. Groen, Sophia Adamia, Teru Hideshima, Danka Cholujova, Jacob P. Laubach, Nikhil C. Munshi, Paul G. Richardson, Kenneth C. Anderson
Journal: Blood (2012)
SAT0367 Can We Differentiate Takayasu Arteritis from Atherosclerosis Using Carotid and Femoral Artery Doppler USG?: Table 1.
Authors: S. Ugurlu, F. Cetinkaya, F.E. Keskin, M. Melikoglu, V. Hamuryudan, I. Fresko, P. Kadioglu, S. Yurdakul, E. Seyahi
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Clinicians can have difficulty in making differential diagnosis between Takayasu arteritis (TA) and atherosclerosis. This is especially true when laboratory evaluations and FDG PET are inconclusive. In addition to that atherosclerosis load is potentially increased in TA (1–2). About 1/3rd of the patients with TA had carotid artery plaques and almost half had diffuse aortic wall calcifications (1–2). <h3>Objectives</h3> We thought that TA might have unique vascular changes distinct from atherosclerosis when evaluated with Doppler ultrasonography (USG). <h3>Methods</h3> Consecutive patients with TA seen in the department of Rheumatology at Cerrahpasa Medical Faculty at Istanbul were studied along with healthy controls. TA patients and healthy controls were aged between 18 and 50 years. Patients with diabetes mellitus (DM) who were followed by the endocrinology outpatient clinic were also studied. For the purposes of this study, no age limit was set for patients with DM. Only females were studied. Traditional atherosclerotic risk factors were also assessed. The radiologist scanned the right and left common, internal and external carotids, carotid bulb as well as the right and left common femoral arteries using Doppler USG. Intima-media thickness (IMT) was measured and resistivity index was calculated. The presence of atherosclerotic plaques and diffuse IMT was assessed. Diffuse IMT was defined as diffuse homogenous intima-media thickening of at least 0.9 mm. <h3>Results</h3> We studied 58 patients with TA (mean age: 43 ±11), 42 patients with DM (mean age: 57 ± 9) and 24 healthy controls (mean age: 41 ± 4). Patients with DM were significantly older and had significantly more atherosclerotic risk factors. The mean carotid IMT was comparable between TA and DM, whereas the mean femoral IMT was significantly lower in TA. The frequency of atherosclerotic plaques in both carotid and femoral arteries was significantly higher among patients with DM (Table). Presence of diffuse wall thickenning of at least 0.9 mm and turbulence were observed almost only among patients with TA. Finally the mean resistivity index was only significantly increased in TA (Table). <h3>Conclusions</h3> Diffuse homogenous increase in IMT, presence of turbulence and higher resistivity index can be considered as suggestive of TA rather than atherosclerosis. This study showed that carotid and femoral artery USG may be helpful in differentiating TA from atherosclerosis. <h3>References</h3> Seyahi E, et al. Ann Rheum Dis. 2006. Seyahi E, et al. Semin Arthritis Rheum. 2013. <h3>Disclosure of Interest</h3> None declared
AB0998 Evaluation of The Effectiveness of Joint Status Self-Assessment by Patients with Rheumatoid Arthritis
Authors: E. Yurova, E. Lygina, S. Yakushin, G. Bagirova, A. Vorobyev
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Objectives</h3> The cooperation in the "doctor-patient" pair is necessary to implement the TREAT TO TARGET conception in the management of rheumatoid arthritis (RA). For greater adherence to treatment, self–control of the disease activity is important for a patient. We have created an original method of training RA patients to self-assess the count of swollen and tender joints correctly. The objective of the research was to study the effectiveness of the method of training patients to self-assess the RA activity. <h3>Methods</h3> The study included 96 patients with the definite diagnosis of RA, according to the ACR/EULAR 2010 diagnostic criteria (88 females; 8 males, mean age 58,1 (51,0; 65,0), the median duration of RA was 8,3 (2,0; 11,5). During the first visit patients were trained using the original method of Disease Activity Self-Assessment (the physician demonstrated the technique of self-assessment of swollen and tender joints and provided the patient with printed guidelines and photos). During the second and third visits with one-month interval, there was an independent control of swollen and tender joint count by a physician and a patient. Statistical significance was analyzed using the McNemar9s test. <h3>Results</h3> Absolute coincidence of the number of tender joints as assessed in parallel by a physician and a patient was observed in 35 cases during the second visit and in 62 (p=0,01) cases during the third visit. Absolute coincidence of the number of swollen joints as assessed in parallel by a physician and a patient was observed in 43 cases during the second visit and in 65 (p=0,01) cases during the third visit. <h3>Conclusions</h3> Our original method of Disease Activity Self-Assessment allows training most patients with RA to assess swollen and tender joints. This is important for self-control of the diseases activity and allows a patent to visit a physician for timely therapy correction. <h3>Disclosure of Interest</h3> None declared