tbuckley/cabot-search huggingface dataset:
P719Different regulation of vascular NOX-1 and NOX-4 expression by interleukin-1beta and angiotensin II
Authors: A Aguado Martinez, O Zhenyukh, T Fischer, C Rodriguez, J Martinez, S Martinez Revelles, R Aras, D Dixon, A M Briones, M Salaices
Journal: Cardiovascular Research (2014)

Purpose. The NADPH oxidase (NOX) is the main source of reactive oxygen species (ROS) which are important in vascular remodeling. Interleukin 1b (IL-1β) and angiotensin II (Ang II) induce different NOX isoforms. We assessed the effects of IL-1β and Ang II on NOX-1 and NOX-4 expression, NOX activity and ROS production, the mechanisms involved and their functional role. Methods. Rat and human aortic smooth muscle cells were stimulated with with IL-1b or Ang II+IL-1b (4, 8 or 24 h). mRNA level was measured by qPCR. NOX-1 and NOX-4 promoter and 3'UTR activity were measured by luciferase assay. HuR cytoplasmic translocation was measured by immunofluorescence and cellular fractionation and the HuR binding to NOX-1 mRNA by immunoprecipitation. NOX activity and ROS production were measured by lucigenin chemiluminescence and with dihydroethidium staining, respectively. Cell proliferation was analyzed by a commercial kit and cell migration by wound healing and transwell. Results. IL-1b (10 ng/ml) increased NOX-1 expression, NOX activity and ROS production, and decreased NOX-4 expression. Ang II (0.1 μM) potentiated NOX-1 expression, NOX activity and ROS production induced by IL-1b and it did not modify IL-1b-induced NOX-4 downregulation. The potentiation of NOX-1 was reduced by ERK1/2, p38MAPK and JNK inhibitors and was accompanied by an increase of mRNA stability. These effects were also inhibited by a HuR inhibitor. Ang II+IL-1b promoted HuR translocation to the cytoplasm and its binding to NOX-1 mRNA. A NOX-1 inhibitor blocked the Ang II+IL-1b-induced cell migration but it did not affect cell proliferation. The decrease of NOX-4 expression induced by IL-1b was blocked by ERK1/2, JNK and PI3K inhibitors and was accompanied by a decrease in the 200 bp proximal promoter activity. These effects were inhibited by cycloheximide. Histone deacetylase inhibitors decreased the basal 200 bp NOX-4 promoter activity and NOX-4 mRNA expression. Conclusions. 1) Ang II potentiates IL-1b-induced vascular expression of NOX-1 through an increase of mRNA stability mediated by HuR. 2) NOX-1-derived-ROS participate in Ang II+IL-1b-induced cell migration. 3) IL-1b induced NOX-4 downregulation mediated by an inducible repressor which binds to the 200 bp proximal promoter. 4) Basal NOX-4 expression depends on deacetylation of a transcription factor that binds to NOX-4 200 bp proximal promoter. 5) An unbalance between NOX-1 and NOX-4 in inflammatory conditions might contribute to vascular remodeling.
Hemopexin Metabolism in Sickle-Cell Disease, Porphyrias and Control Subjects — Effects of Heme Injection
Authors: R. Dean Wochner, Isaias Spilberg, Atsushi Iio, Ham Heng Liem, Ursula Muller-Eberhard
Journal: New England Journal of Medicine (1974)

To study hemopexin metabolism the turnover of 125I-labeled plasma hemopexin was determined in 10 control subjects, five patients with erythropoietic protoporphyria, one with porphyria cutanea tarda, and two with sickle-cell disease. Hemopexin half-life in the control subjects (mean ± S.D.) was 7.1 ± 1.0 days, and fractional catabolic rates were 23.6 ± 3.0 per cent of the plasma pool per day. 125I-hemopexin catabolism was accelerated over controls in each of these illnesses, with fractional catabolic rates of 27.6 ± 2.1 per cent in erythropoietic protoporphyria (p<0.05), 35 per cent in the patient with porphyria cutanea tarda and 41 and 32 per cent in the patients with sickle-cell disease. Since the calculated synthetic rates for hemopexin fell within the normal range in these patients, reduction in plasma hemopexin levels observed was accounted for by accelerated breakdown. Injection of heme into a control subject produced marked acceleration of hemopexin catabolism, providing further documentation for the role of hemopexin in the clearance of plasma heme in man. (N Engl J Med 290:822–826, 1974)
SUSTAINED IMPROVEMENT IN CARDIAC FUNCTION WITH SINGLE DOSE CIMAGLERMIN ALFA (GGF2) IN HUMAN LEFT VENTRICULAR SYSTOLIC DYSFUNCTION
Authors: Daniel J. Lenihan, Evan Brittain, Carrie Lenneman, Andrew Eisen, Ping Zhao, Jen Iaci, Lisa Mendes, Stephen Frohwein, Douglas Sawyer, Anthony Caggiano
Journal: Journal of the American College of Cardiology (2015)
DOES THE SMOKER'S PARADOX EXIST IN PATIENTS WITH ST-ELEVATION MYOCARDIAL INFARCTION UNDERGOING PRIMARY PERCUTANEOUS CORONARY INTERVENTION?
Authors: Tanush Gupta, Dhaval Kolte, Sahil Khera, Prakash Harikrishnan, Wilbert Aronow, Diwakar Jain, Sachin Sule, Ali Ahmed, Howard Cooper, William Frishman, Deepak Bhatt, Gregg Fonarow, Julio Panza
Journal: Journal of the American College of Cardiology (2016)
Angiogenesis in multiple myeloma: correlation between in vitro endothelial colonies growth (CFU-En) and clinical–biological features
Authors: M Dominici, D Campioni, F Lanza, M Luppi, P Barozzi, S Pauli, R Milani, F Cavazzini, M Punturieri, R Trovato, G Torelli, G Castoldi
Journal: Leukemia (2001)
Rheumatic Syndromes of Myxedema
Authors: John H. Bland, John W. Frymoyer
Journal: New England Journal of Medicine (1970)

In 74 patients, referred between 1954 and 1967 to our Rheumatology Unit because of arthritis, we suspected hypothyroidism on clinical grounds. Laboratory confirmation was obtained in 38; five had no objective signs of arthritis, and 22 were excluded as having other causes for rheumatic symptoms and signs. The remaining 11 patients had a variety of rheumatic syndromes as their principal manifestation of myxedema. Diagnoses with which myxedema joint disease was confused were serum-negative rheumatoid arthritis, intervertebral disk disease, osteoarthritis, fibrositis, psychoneurosis and nonspecific "arthritis" or rheumatism. All patients recovered completely on thyroid-replacement therapy.
Abstract 815: The role of spleen tyrosine kinase (SYK) in paclitaxel resistant ovarian cancer
Authors: Stephanie Gaillard, Alexander Stoeck, Ben Davidson, Tian-Li Wang, Ie-Ming Shih
Journal: Cancer Research (2012)

Abstract The development of resistance to paclitaxel represents a general challenge in clinical oncology, particularly in ovarian serous carcinomas (OSCs), the most common and lethal form of ovarian cancer. Initial treatment with a taxane and platinum combination regimen is standard and while the majority of women will achieve a major response to this treatment, the mortality of ovarian cancer is directly related to the tumor's development of chemoresistance. Using a proteomic approach to comprehensively compare the protein expression profiles of primary and recurrent OSC clinical samples, our laboratory previously identified several novel candidate proteins associated with the development of recurrent OSC. In this study, we focus on evaluating one of these proteins, spleen tyrosine kinase (SYK), a non-receptor tyrosine kinase, and its role in the maintenance of paclitaxel resistance in OSC. We have determined that increased expression of SYK in OSC tumor cells obtained from ascites and/or pleural effusion fluid is associated with a poorer response to chemotherapy. Using a cell culture system, we found that two OSC cell lines show an increased expression of SYK after development of in vitro resistance to paclitaxel treatment. More importantly, we demonstrate that SYK small compound inhibitors significantly resensitize the tumor cells to paclitaxel. Our results suggest that SYK plays a role in maintaining paclitaxel resistance in OSC. Recently an oral SYK inhibitor was found to be safe and effective in treating rheumatoid arthritis and non-Hodgkin lymphoma; our results suggest a potential translational use for this inhibitor as a chemosensitizing agent in patients with OSC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 815. doi:1538-7445.AM2012-815
S49 Increased skeletal muscle-specific microRNA-1 in the blood of COPD patients
Authors: A. V. J. Donaldson, A. Lewis, A. Natanek, W. D. Man, P. Kemp, M. I. Polkey
Journal: Thorax (2011)

<h3>Introduction</h3> Quadriceps muscle dysfunction is an important prognostic comorbidity in COPD. MicroRNAs (miRs) are small non-coding RNAs that regulate gene expression. Skeletal muscle expresses a number of tissue-specific microRNA including miR-1, which modulates muscle phenotype. MicroRNAs can be secreted from cells and maintained in blood within exosomes. Elevated levels of circulating miR-1 have been demonstrated in a number of human and animal models of muscle disease. We hypothesised that plasma levels of miR-1 would be elevated in COPD patients and would correlate with important physiological parameters. <h3>Methods</h3> 103 COPD patients and 25 controls were studied. MiR-1 was quantified in stored plasma samples using q-RT PCR.1 MiR-16 and miR-122 were quantified as negative controls. Results were normalised to an exogenous spiked-in control. <h3>Results</h3> Characteristics as mean (SD); COPD patients: M: 67, F: 36, age=66.47 (8.4), FEV<sub>1</sub> % pred= 43.5 (18.6), 6-minute walk (6MW) = 394 (120). Controls: M: 14, F: 11, age=67 (8.1), FEV<sub>1</sub> % pred=111.2 (13.1), 6MW=613 (83). Plasma miR-1 was significantly elevated in COPD patients, p=0.002. There was no difference in miR-16 and miR-122. MiR-1 was negatively associated with FEV<sub>1</sub> % predicted (r =−0.3, p&lt;0.001) and with Tlco (r=−0.3, p&lt;0.001), but it was not possible to distinguish between GOLD stages using ANOVA. However, if patients were sub-divided into early GOLD stage COPD (1 and 2) or late GOLD stage COPD (3 and 4), miR-1 was significantly higher in the latter group (p=0.02). The plasma level of miR-1 was inversely correlated with daily activity measured as locomotion time (r= −0.25, p&lt;0.01) but miR-1 levels were not associated with any muscle phenotype or with muscle-specific gene expression. <h3>Conclusion</h3> Our results show that stable COPD patients have elevated plasma levels of muscle-specific miR-1. The increase in miR-1 may be due to increased muscle degradation or turnover in the COPD patients studied. Our work raises the possibility of using other muscle-specific microRNAs in the future as potential biomarkers of muscle dysfunction in patients with COPD.
An Unusual Case of Abdominal Pain
Authors: Russell Goodman, Paul F. Dellaripa, Amy Leigh Miller, Joseph Loscalzo
Journal: New England Journal of Medicine (2014)

A 41-year-old man presented to the ED with abdominal pain. The pain was excruciating and sudden in onset, originating in his right lower quadrant and radiating to his right groin and flank. He reported nausea and one episode of nonbloody, nonbilious emesis.
Abstract 2530: Incidence trends of childhood and adolescent melanoma in the United States from 1973-2009.
Authors: Jeannette Wong, Jenine Harris, Carlos Rodriguez-Galindo, Kimberly Johnson
Journal: Cancer Research (2013)

Abstract OBJECTIVE: Melanoma in children and adolescents is rare but, similar to adults, has been reported to be increasing. To gain insight into possible reasons underlying increased number of cases, we analyzed trends in melanoma incidence diagnosed between the ages of 0-19 years among United States whites by sex, stage, age at diagnosis, and primary site. We also investigated incidence trends by ultraviolet B radiation (UVB) exposure levels. METHODS: Using Surveillance, Epidemiology, and End Results (SEER) program data (1973-2009), we calculated age-adjusted incidence rates, annual percent changes, and 95% confidence intervals (CIs) for each category of interest. Incidence trends were also evaluated using joinpoint and loess regression models. SEER registries were categorized with respect to low or high UVB radiation exposure. RESULTS: From 1973-2009, 1,230 children of white race were diagnosed with malignant melanoma. Overall, pediatric melanoma increased by an average of 2% per year (95% CI, 1.4%-2.7%). Females, 15-19 year olds, and individuals with low UVB exposure had significantly higher incidence rates than males, younger children, and those living in SEER registries categorized as high UVB. Over the study period, males experienced increased incidence rates for melanoma on the face and trunk, and females on the lower limbs and hip. The only decreased incidence trend we observed was among 15-19 year olds in the high UVB exposure group from 1985-2009. Loess curves indicated similar patterns. CONCLUSIONS: These results may help elucidate possible risk factors for adolescent melanoma, but further individual-level studies will be necessary to determine the reasons for increasing incidence trends. Citation Format: Jeannette Wong, Jenine Harris, Carlos Rodriguez-Galindo, Kimberly Johnson. Incidence trends of childhood and adolescent melanoma in the United States from 1973-2009. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2530. doi:10.1158/1538-7445.AM2013-2530
Exceptional Intelligence in a Mongoloid Child of a Family with a 13–15/Partial 21 (D/Partial G) Translocation
Authors: Sara C. Finley, Wayne H. Finley, Clarence J. Rosecrans, Carey Phillips
Journal: New England Journal of Medicine (1965)

PATIENTS with Down's syndrome (mongolism) usually have 47 chromosomes and appear to be trisomic for No. 21 as first described by Lejeune, Gauthier and Turpin1 in 1959. However, it was soon found that some patients who satisfied the same clinical criteria had 46 chromosomes, including 1 of a new type originated through a translocation process. The first was reported by Polani and his associates2 in 1960 and was interpreted as a new chromosome resulting from a reciprocal translocation between 1 member of Group 21–22 (G) and 1 of Group 13–15 (D). A second type, interpreted as a translocation between 1 . . .
In vivo imaging of Lgr5-positive cell populations using confocal laser endomicroscopy during early colon tumorigenesis
Authors: Jin Choi, Jun Kim, Myunghwan Choi, Yi Kim, Seok Yun
Journal: Endoscopy (2014)

<b>Background and study aims:</b> A diagnostic molecular marker for pre-neoplastic lesions, particularly before polyposis, is still lacking. Lgr5 has been broadly accepted as a marker for intestinal cancer stem cells. The aim of this study was to investigate the monitoring of Lgr5<sup> + </sup> cells as a useful tool for the early diagnosis of premalignant lesions before polyp formation. <b>Methods:</b> In vivo molecular imaging was performed to examine colon tumorigenesis in <i>Lgr5-EGFP</i> mice treated with azoxymethane and dextran sodium sulfate. eGFP<sup> +</sup> Lgr5<sup> +</sup> regions in the descending colon were longitudinally monitored using side-view confocal endomicroscopy. Based on the eGFP signal intensity on the luminal surface, polyps were classified into two groups – Lgr5-high and Lgr5-low. White light colonoscopy was used to monitor polyp formation. <b>Results:</b> Approximately 75 % of the polyps originated from foci containing Lgr5-eGFP<sup> +</sup> cells, whereas 25 % of the polyps emerged from Lgr5<sup> –</sup> foci. Among eGFP<sup> +</sup> foci, Lgr5-high foci grew faster than Lgr5-low foci. <b>Conclusions:</b> Polyps developed at Lgr5<sup> +</sup> regions. Luminal Lgr5 expression was correlated with the growth rate of early-stage adenomas. Lgr5 is a promising molecular marker for the early diagnosis of colon tumors.
Cancer Information Seekers Cruise the "Highways"
Authors: K. Nagy
Journal: JNCI Journal of the National Cancer Institute (1994)

Journal Article Cancer Information Seekers Cruise the “Highways” Get access Kate Nagy Kate Nagy Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 86, Issue 15, 3 August 1994, Pages 1115–1118, https://doi.org/10.1093/jnci/86.15.1115 Published: 03 August 1994
P170 INTRAHEPATIC QUASISPECIES HETEROGENEITY AS A PREDICTOR FOR VIROLOGICAL RELAPSE AFTER NUCLEOTIDE ANALOGUES WITHDRAWAL IN HBeAg POSITIVE CHRONIC HEPATITIS B PATIENTS: A PROSPECTIVE STUDY
Authors: Q.R. Gan, C. Pan, M.H. Lin, R.S. Lin, Q. Li, L.F. Yao, D.Q. Zhang, Q.G. Li, L. Chen
Journal: Journal of Hepatology (2014)
Equitable Allocation of HLA-Compatible Kidneys for Local Pools and for Minorities
Authors: Steve Takemoto, Paul I. Terasaki, David W. Gjertson, J. Michael Cecka
Journal: New England Journal of Medicine (1994)

The methods used to allocate cadaveric kidneys in the United States have been criticized as being unfair to minorities because of an overemphasis on HLA matching. We evaluated a new HLA-matching method that might alleviate this problem.
UPTAKE OF FIBRIN MONOCLONAL ANTIBODY (MAb) BY A MOUSE CANCER WITH COAGULATIVE PROPERTIES.
Authors: G. L. DeNardo, S. J. DeNardo, N. P. Miyao, G. P. Adams, U. C. Davis
Journal: Clinical Nuclear Medicine (1986)

DeNardo, G. L.; DeNardo, S. J.; Miyao, N. P.; Adams, G. P.; Davis, U. C. Author Information
PWE-242 Accessing information on IBD: a survey of sources and reliability of internet resources
Authors: R A Ley Greaves, L F Wilkinson, T Orchard
Journal: Gut (2012)

<h3>Introduction</h3> Crohn9s and Ulcerative Colitis (UC) are chronic diseases, often requiring lifelong treatment. They can be variable therefore accurate and useful patient information is vital. With an increasing number of sources this can be a cause of confusion for patients. As part of a service evaluation this study examined the information given to patients by doctors, other sources used, how they chose their information and their accuracy. <h3>Methods</h3> 100/113 patients agreed to answer a short survey. Topics were: Sources of information, internet usage, checks made on information and reasons for choices. Separately an internet search was performed with three major search engines using five search terms (IBD, UC, Crohn9s, Ulcerative colitis and inflammatory bowel disease). From the top 10 hits on each 22 websites that were relevant to the search term were then checked against 5 criteria for accreditation of information. <h3>Results</h3> Of 100 patients (43 male, 57 female) with an average age of 44 (18–81). There were 47 Crohn9s and 53 UC patients. 65% had suffered for over 5 years. 48% wanted more information than given in clinic and 91% had sought further information. Of those who sought further information 90% used the internet, 55% patient leaflets and 25% used books. Sources used varied with age, 78% of over 65s didn9t use the internet while only 6% of under 30s doing research didn9t. Leaflet use in the under 30s was 48% while being 78% in the over 65s. Using the search terms in Google, Yahoo and bing an average of 1, 1.5 and 2 sponsored advertising links were highest ranked respectively. 67% of those using the internet picked websites on their search engine ranking with 20% picking on recommendation. 40% made no checks on the information they found whereas 23% said they “read around”. Of the 22 relevant websites 7 filled 5 accreditation criteria and 6 scored 2 or less. Crohn9s and Colitis UK (NACC) website scored highly for accreditation and 45% of patients used it 62% of whom had been recommended it by their doctor. However its mean search engine ranking was 7th and it only appeared in the top ten in 9/12 searches. In the free text section a number of patients commented on concerns about inaccurate information. <h3>Conclusion</h3> The results show patient information is mainly sourced from the internet and patient leaflets. Internet information is of varying quality and search engine rankings are influenced by sponsored links directed at IBD patients as opposed to accredited sites. Data on use of the NACC website with and without recommendation show simple measures such as a health care professional taking time to advise a patient may direct them to towards better information and away from lower quality potentially misleading sites. Many patients commented that websites with unsubstantiated information caused them significant concern. <h3>Competing interests</h3> None declared.
A Comparison of Seven Antiarrhythmic Drugs in Patients with Ventricular Tachyarrhythmias
Authors: Jay W. Mason
Journal: New England Journal of Medicine (1993)

The relative efficacies of various antiarrhythmic drugs in the treatment of ventricular tachyarrhythmias are not well known. This study examined the effectiveness of imipramine, mexiletine, pirmenol, procainamide, propafenone, quinidine, and sotalol in patients with ventricular tachyarrhythmias who were enrolled in the Electrophysiologic Study versus Electrocardiographic Monitoring trial.
AB1007 Increased Vascular Wall Inflammation in Patients with Active Rheumatoid Arthritis as Measured by an 18F-Fdg-Pet/Ct Scan
Authors: R. Agca, A.M. Sijl, Y.M. Smulders, A.E. Voskuyl, C. van der Laken, R. Boellaard, K.D. Lensen, M.T. Nurmohamed
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Patients with rheumatoid arthritis (RA) have an elevated risk of developing cardiovascular disease (CVD). Like active RA, atherosclerosis is an inflammatory process. There are indications that aortic inflammation in atherosclerosis can be detected on <sup>18</sup>F-Fluorodeoxyglucose-positron emission tomography/computed tomography (<sup>18</sup>F-FDG-PET/CT). <h3>Objectives</h3> To quantify <sup>18</sup>F-FDG uptake in large arteries of RA patients using PET/CT, as potential reflection of vascular wall inflammation in areas of atherosclerosis, and its association with disease activity in patients with RA as compared to controls. <h3>Methods</h3> <sup>18</sup>F-FDG-PET/CT was performed in patients with active RA (DAS28 &gt;4.0; n=29) and in controls with osteoarthritis (OA; n=11). Semi-quantitative FDG-uptake was determined by calculation of the mean standardized uptake value (SUV) and tissue-to-background ratio (TBR) using <sup>18</sup>F-FDG activity in the vena cava as background. One volume of interest (VOI) of an arterial segment with maximum <sup>18</sup>F-FDG uptake was used for SUV calculation (focal arterial uptake). Total arterial uptake was estimated by using the mean of all focal arterial uptakes in all arteries. <h3>Results</h3> Focal as well as total arterial uptake of <sup>18</sup>F-FDG was the highest in patients with RA. SUV was significantly higher in RA for the thoracic aortic tract, the abdominal aorta and the femoral arteries as compared to OA controls. CRP was associated with an increased total arterial uptake as well as an increased focal arterial uptake in the thoracic aortic tract, the abdominal aorta, the iliac arteries and the femoral arteries. DAS28 &gt;2,6 was also correlated with total arterial uptake and uptake in the thoracic aortic tract, the abdominal aorta and the iliac arteries. There were no significant differences in TBR between RA and OA. <h3>Conclusions</h3> Increased focal vascular wall uptake of <sup>18</sup>F-FDG as sign of vascular inflammation was found in several arterial segments of RA patients. CRP and clinical RA activity were correlated with <sup>18</sup>F-FDG uptake in most of these arteries. Overall, total arterial uptake was higher in RA patients and it was correlated with CRP and disease activity. The lack of differences in TBR measurements requires further study. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4185
897 INTERLEUKIN-28b CC GENOTYPE PREDICTS TREATMENT EARLY RESPONSE AND CT/TT GENOTYPES PREDICT NON RESPONSE IN HCV GENOTYPE 3 INDIAN PATIENTS
Authors: A. Gupta, S. Sukriti, P. Gautam, N. TrehanPati, S. Sharma, S. Hissar, V. Midha, T. Singh, A. Sood, S.K. Sarin
Journal: Journal of Hepatology (2013)
BLEEDING RISK STRATIFICATION USING MICROCHIP FLOW-CHAMBER SYSTEM IN PATIENTS RECEIVING MULTIPLE ANTITHROMBOTIC AGENTS
Authors: Shinya Ichikawa, Kengo Tsukahara, Eiichi Akiyama, Nobuhiko Maejima, Noriaki Iwahashi, Kiyoshi Hibi, Masami Kosuge, Toshiaki Ebina, Satoshi Umemura, Kazuo Kimura
Journal: Journal of the American College of Cardiology (2016)