tbuckley/cabot-search huggingface dataset:
Abstract 1896: Bioluminescent assays for investigating cell metabolism.
Authors: Donna Leippe, Mary Sobol, Sarah Duellman, Jolanta Vidugiriene, Wenhui Zhou, Gediminas Vidugiris, Troy Good, Laurent Bernad, Poncho Meisenheimer, James Cali
Journal: Cancer Research (2013)

Abstract Cell metabolism is an important and expanding area of research in the field of cancer biology. Simple, rapid assays for studying cellular metabolic pathways, their enzymes, metabolites and cofactors can facilitate these studies. We have developed two novel bioluminescent technologies, one for monitoring cell viability, and the other for monitoring changes in cellular nicotinamide adenine dinucleotides, that can facilitate the study of cell metabolism and its regulation. The bioluminescent cell viability assay is based on the metabolic activity of live cells. Metabolically active cells reduce a proluminogenic substrate in vivo which is then detected by a luciferase enzyme in the media. The luminescence output is correlated with the number of viable cells. The assay is non-toxic, sensitive (detects less than 10 cells/well) and has a large dynamic range (S/B&amp;gt;100). The key, differentiating feature of this assay is the incorporation of the recently developed stable and very bright NanoLuc™ luciferase and its profurimazine substrate, which allows changes in viability to be monitored in real-time and continuously, over extended periods of time, from the same sample. Three homogeneous, one-step, bioluminescent assays have been developed for the detection of nicotinamide adenine dinucleotides, specific for: (1) reduced forms NADH and NADPH, (2) non-phosphorylated forms NAD and NADH, and (3) phosphorylated forms NADP and NADPH. The assays are based on the reaction of the enzyme diaphorase with a proluciferin substrate, which in the presence of NADH or NADPH is converted to luciferin, a substrate for luciferase. These assays are sensitive (LOD ≤ 50 nM) and have large maximum signal windows (S/B &amp;gt; 100), two advantages over currently available colorimetric and fluorescent detection methods. They are also robust, and amenable to automated and high-throughput protocols (Z’ &amp;gt; 0.8). The high sensitivity, one-step reagent addition, and no requirement for sample processing, permit an in-well protocol for the assay of cells directly in culture plate wells. Changes in cellular NAD levels can be rapidly monitored, as demonstrated using FK866, an inhibitor of NAD biosynthesis. FK866 caused a decrease in NAD levels in multiple cell types with good signal windows (S/B &amp;gt; 10) and expected pharmacology (IC50 values = 1-10 nM). Citation Format: Donna Leippe, Mary Sobol, Sarah Duellman, Jolanta Vidugiriene, Wenhui Zhou, Gediminas Vidugiris, Troy Good, Laurent Bernad, Poncho Meisenheimer, James Cali. Bioluminescent assays for investigating cell metabolism. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1896. doi:10.1158/1538-7445.AM2013-1896
The arterial supply of the major duodenal papilla and its relevance to endoscopic sphincterotomy
Authors: S. Mirjalili, M. Stringer
Journal: Endoscopy (2011)

Background and study aims: Arterial bleeding from the major duodenal papilla is an uncommon but potentially life-threatening complication of endoscopic sphincterotomy. We investigated the arterial blood supply of the papilla to determine whether there might be a safer region for sphincterotomy.
Abstract 778: Suppression of lung adenocarcinoma progression by NKX2-1 is dependent on p53 Status.
Authors: Huei Lee, Po-Ming Chen, Tzu-Chin Wu, Yawen Cheng, Chih-Yi Chen
Journal: Cancer Research (2013)

Abstract This article investigates the mechanistic aspects of NKX2-1 as a tumour suppressor and its oncogenic properties in lung adenocarcinoma. We show that NKX2-1 is a transcriptional factor that directly regulates p53 transcription. In p53 wild-type cells, NKX2-1 interacts with SP1 to down-regulate IKKβ transcription. In p53 mutated cells, NKX2-1 elevates the mutant p53/NF-Y complex to up-regulate IKKβ transcription. NKX2-1-mediated IKKβ expression is responsible for the efficacy of soft agar growth, invasion, and xenograft lung tumour nodule formation. Moreover, tumour aggressiveness attenuated by NKX2-1-mediated IKKβ is predominately through the shuffling of Foxo3a between the cytosol and nucleus of lung cancer cells to modulate E-cadherin expression. A consistent association of NKX2-1 with IKKβ and E-cadherin expression was observed in tumours from lung adenocarcinoma patients. P53 mutated tumours with high NKX2-1 expression have poorer overall survival (OS) and relapse-free survival (RFS) rates than those with low-NKX2-1 expression. Conversely, p53 wild-type tumours with high-NKX2-1 tumours have more favourable OS and RFS rates than those with low-NKX2-1 expression. As such, we demonstrate that aberrant transcription of IKKβ underlines the ability of NKX2-1 to act as a tumour suppressor or to display oncogenic properties in lung adenocarcinoma. Citation Format: Huei Lee, Po-Ming Chen, Tzu-Chin Wu, Yawen Cheng, Chih-Yi Chen. Suppression of lung adenocarcinoma progression by NKX2-1 is dependent on p53 Status. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 778. doi:10.1158/1538-7445.AM2013-778
Intracardiac Shunts after Penetrating Wounds of the Heart
Authors: Charles P. Summerall, William H. Lee, John A. Boone
Journal: New England Journal of Medicine (1965)

IN the past half century, since Rehn's early (1896) repair of a cardiac laceration, success in the restoration of wounded hearts has been a vivid chapter in surgical progress. Maguire and Griswold,1 seventeen years ago, could report a mortality of only 8.7 per cent in 23 patients who survived their cardiac wounds long enough to permit thoracotomy. In the era of cardiac catheterization and openheart surgery the recognition and repair of intracardiac shunts caused by blunt or penetrating wounds have added further drama to the story. It seems worthwhile to record further examples of this unusual clinical problem.Case Reports . . .
Defining Why Cancer Develops in Children
Authors: John M Maris
Journal: New England Journal of Medicine (2015)

It has been more than 40 years since Knudson ushered in the modern era of cancer genetics with his seminal “two hit” hypothesis to explain familial cancer syndromes and provide a mathematical model for the biallelic inactivation of tumor-suppressor genes — with the first hit being inherited and the second occurring somatically to initiate tumorigenesis.1 Notably, he studied rare childhood cancers to develop his initial model, which proved to be remarkably accurate for both pediatric-onset and adult-onset heritable cancers.Since the identification in 1986 of loss-of-function mutations in the gene RB1 that cause hereditary retinoblastoma,2 more than 100 cancer-predisposition genes . . .
Abstract 3643: Phosphatidylserine-targeting antibody combined with Sorafenib has strong anti-tumor activity against human hepatocellular carcinomas in mice
Authors: Xiaoyun Cheng, Philip E. Thorpe
Journal: Cancer Research (2011)

Abstract Hepatocellular Carcinoma (HCC) is the third leading cause of cancer-related deaths in the world. The incidence of the HCC is increasing in the United States. HCC is refractory to most forms of therapy. Sorafenib is the only chemotherapeutic agent approved for the treatment of advanced HCC. Therefore, there is a huge unmet need for a new systemic therapy for HCC patients. A promising approach would be to combine sorafenib with bavituximab to treat HCC. Bavituximab is a vascular targeting antibody that is in Phase IIb clinical trials in patients with non-small cell lung cancer (NSCLC). Bavituximab targets phosphatidylserine (PS), which is normally intracellular but becomes exposed on the external surface of vascular endothelium in tumors. Bavituximab binds to PS-expressing tumor vascular endothelial cells and causes monocytes and macrophages to attack the vessels by antibody-dependent cellular cytotoxicity (ADCC). This results in death of tumor cells through deprivation of oxygen and nutrients. Chemotherapy and irradiation increase the exposure of PS on tumor vessels and enhance the anti-tumor activity of bavituximab-related antibodies in multiple rodent tumor models. We hypothesized that sorafenib would also induce exposure of PS on HCC vasculature because it interferes with anti-apoptotic signaling by VEGF and other angiogenic growth factors. We found that sorafenib induced PS exposure on human microvascular endothelial cells in vitro and in vivo. Treatment of mice bearing subcutaneous C3A, Huh7 or PLC/PRF/5 HCC tumors with sorafenib increased PS exposure on tumor vessels from 21% to 51% in PLC/PRF/5 tumors, from 22% to 67% in C3A tumors, and from 32% to 52% in Huh7 tumors. Combining a murine version of bavituximab with sorafenib significantly improved the anti-tumor effect in the PLC/PRF/5 xenograft model. The combination inhibited tumor growth by 95%, as compared with 80% for sorafenib alone (p&amp;lt;0.05). Bavituximab in combination with sorafenib has promise as a new systemic therapy for the treatment of advanced HCC patients. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3643. doi:10.1158/1538-7445.AM2011-3643
AB1114 Juvenile idiopathic arthritis: Can mother mood be a determinant factor in the child illness management?
Authors: A. Officioso, G. Griso, G. Russo, C. Storace, M. Alessio
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Juvenile Idiopathic Arthritis (JIA) is a chronic disease that causes children to have swelling, pain, and stiffness in their joints. JIA occurs before the age of 16. In particular child’s social, emotional and also cognitive development is not always in accordance with their age and peers. It isn’t unusual for someone who is living with pain, fatigue and loss of function to feel overwhelmed, depressed and anxious about the future. All chronic illnesses, have wide-reaching effects that impact not only the child, but other family members as well. Families experiencing childhood chronic illness must adapt to care giving burdens, stress, and anxiety demands. Being the parent of a child with any chronic illness can be difficult. Often parents are involved in guiding their teenager into taking more control and learning how to care for his or her own disease. And this condition can trigger depression in a family member. <h3>Objectives</h3> to investigate some psychological issues related to JIA, in particular, the relation existing between mother’s depression and child’s depression <h3>Methods</h3> Participants included 30 children (M=10, F=20), aged 7,9±3,4 and 30 mothers, aged 41,5±4,9 matched for social class. The level of depression of the children was investigated by Kovac’s children’s depression inventory (CDI). This test provides information about the youth’s scores and how they compare with the scores of the normative sample of non-clinical individuals. The psychological status of their mothers was assessed by the psychological general well-being (PGWB) covering the 6 affective states of health-related quality of life, anxiety, depressed mood, sense of positive well-being, self-control, general health, and vitality. Measures of unfunctional disability were completed. CDI results are been incorporated with other information as a direct clinical observation of the youth, and information about the youth’s background, family history, and school adjustment. <h3>Results</h3> Physical and psychological domains concerning individual’s overall perception of quality of life resulted impaired in mothers of JV children. The level of depression of the child can be correlated with the mother’s level of quality of life. <h3>Conclusions</h3> Adolescents experience higher levels of depression and a lower quality of life as they realize the impact their disease will have on their life. Anxiety and stress are high also among parents as they deal with the worries related to their adolescent’s growing independence and their own struggles over allowing the teenager to have that freedom to control his or her own health. <h3>Disclosure of Interest</h3> None Declared
THE BACTERIOLOGY OF WHOOPING COUGH.
Journal: JAMA (1897)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Prophylaxis with Single-Dose Doxycycline for the Prevention of Lyme Disease after an<i>Ixodes scapularis</i>Tick Bite
Authors: Robert B. Nadelman, John Nowakowski, Durland Fish, Richard C. Falco, Katherine Freeman, Donna McKenna, Peter Welch, Robert Marcus, Maria E. Agüero-Rosenfeld, David T. Dennis, Gary P. Wormser
Journal: New England Journal of Medicine (2001)

It is unclear whether antimicrobial treatment after an Ixodes scapularis tick bite will prevent Lyme disease.
Visualization of Fatal Hepatic Necrosis on Bone Scan
Authors: MAKOTO HOSONO, KIKUO MACHIDA, NORINARI HONDA, TAKEO TAKAHASHI, NOBUYUKI OHTAWA
Journal: Clinical Nuclear Medicine (2003)

HOSONO, MAKOTO M.D., PH.D.; MACHIDA, KIKUO M.D., PH.D.; HONDA, NORINARI M.D.; TAKAHASHI, TAKEO M.D.; OHTAWA, NOBUYUKI M.D. Author Information
AB0263 Impact Evaluation of A Doses Optimization Strategy for Biological Products in Structural Damage Measured by Low-Field MRI in Rheumatoid Arthritis Patients: Table 1
Authors: M.D. Ruiz Montesino, C. Vargas Lebron, V. Moreira Navarrete, F. Navarro Sarabia
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Introduction</h3> Inappropriate use of antimicrobials in hospitals contributes to antimicrobial resistance. Antimicrobial stewardship (AMS) interventions aim to improve antimicrobial prescribing, but they are often resource and personnel intensive. Computerised decision supportsystems (CDSSs) seem a promising tool to improve antimicrobial prescribing but have been insufficiently studied in clinical trials. <h3>Methods and analysis</h3> The COMPuterized Antibiotic Stewardship Study trial, is a publicly funded, open-label, cluster randomised, controlled superiority trial which aims to determine whether a multimodal CDSS intervention integrated in the electronic health record (EHR) reduces overall antibiotic exposure in adult patients hospitalised in wards of two secondary and one tertiary care centre in Switzerland compared with ‘standard-of-care’ AMS. Twenty-four hospital wards will be randomised 1:1 to either intervention or control, using a ‘pair-matching’ approach based on baseline antibiotic use, specialty and centre. The intervention will consist of (1) decision support for the choice of antimicrobial treatment and duration of treatment for selected indications (based on indication entry), (2) accountable justification for deviation from the local guidelines (with regard to the choice of molecules and duration), (3) alerts for self-guided re-evaluation of treatment on calendar day 4 of antimicrobial therapy and (4) monthly ward-level feedback of antimicrobial prescribing indicators. The primary outcome will be the difference in overall systemic antibiotic use measured in days of therapy per admission based on administration data recorded in the EHR over the whole intervention period (12 months), taking into account clustering. Secondary outcomes include qualitative and quantitative antimicrobial use indicators, economic outcomes and clinical, microbiological and patient safety indicators. <h3>Ethics and dissemination</h3> Ethics approval was obtained for all participating sites (Comission Cantonale d9Éthique de la Recherche (CCER)2017–00454). The results of the trial will be submitted for publication in a peer-reviewed journal. Further dissemination activities will be presentations/posters at national and international conferences. <h3>Trial registration number</h3> NCT03120975; Pre-results.
John Brown Morris
Authors: Rae Morris
Journal: BMJ (2016)

John Brown Morris was born in Glasgow and an only child. He attended Pollokshaws primary school and was evacuated to Braehead, Lanarkshire (near Carstairs), during the war. When he returned to Glasgow he began his secondary education at Hutchesons’ Grammar School for boys, where he studied Classics. Although he never studied the sciences, he was accepted to read medicine at Glasgow University in 1948. 

After qualifying he was initially posted to Stobhill Hospital, then Law Hospital, Carluke, where he met his wife, Rae, who was also doing her preregistration year. The couple married in October 1955 and moved to Ballykelly, Northern Ireland, …
Fulminant Coxsackieviral Myocarditis
Authors: In-Whan Seong, Seong-Choon Choe, Eun-Seok Jeon
Journal: New England Journal of Medicine (2001)

The Sounding Board by Bedell et al. on the doctor's letter of condolence (April 12 issue) 1 was distressing to read.My wife of 40 years died at a prestigious teaching hospital.For nearly four years she had struggled with carcinoma of the fallopian tube.There were 26 hospital admissions for surgery, chemotherapy, or emergency rehydration.When standard approaches ultimately failed, she managed, largely through her own research, to become enrolled at another distinguished hospital in a high-risk experimental protocol: high-dose chemotherapy followed by transplantation of autologous bone marrow.But the regimen turned out to be lethal.She died after three days in the emergency cardiac unit.Neither the hospital management nor the attending physician or anyone from the house staff ever troubled to write or telephone to express sympathy or offer an account of what had gone so wrong those last disastrous days.The effect of that bizarre silence was to make me wonder whether some monumental mistake might have been made in preparing the dose that was to end her life.The next of kin are entitled to some expression of sympathy or concern, even when it is not deeply felt.Those close to the deceased can only be baffled, resentful, or suspicious when no condolences are expressed.
Reduced intensity conditioning allogeneic stem cell transplantation for patients with acute myeloid leukemia: long term results of a ‘donor’ versus ‘no donor’ comparison
Authors: M Mohty, H de Lavallade, J El-Cheikh, P Ladaique, C Faucher, S Fürst, N Vey, D Coso, A-M Stoppa, J-A Gastaut, C Chabannon, D Blaise
Journal: Leukemia (2008)
Bisphosphonates and Atypical Femoral Fractures
Authors: Samantha Pozzi, Noopur Raje
Journal: New England Journal of Medicine (2010)

on the epidemiol-ogy of subtrochanteric fractures of the femoral shaft in more than 14,000 participants from ran -domized, controlled trials. In our view, the article has two major limitations. The literature suggests that these fractures are seen in some patients who have used bisphosphonates for a long time. How -ever, only about 1000 patients in the Black study had taken bisphosphonates for more than 4.5 years, and 40% of the fractures occurred within this group. Second, the literature suggests that in patients taking bisphosphonates, these fractures have a characteristic radiographic pattern. The au -thors did not have access to radiographs that could confirm whether the fractures had the spiral ap -pearance of traumatic or typical osteoporotic frac -tures or the characteristic appearance seen in as -sociation with prolonged use of bisphosphonates: cortical thickening (lateral greater than medial), cortical beaking in the upper third and middle third of the femoral shaft, and transverse to oblique fracture — all often evident in both legs. We agree with the authors that further studies are needed.Stuart L. Silverman, M.D.
FRI0016 Critical role for the IL-23/TNF-alpha axis during TLR2/NOD2 mediated acute joint inflammation
Authors: F. Cornelissen, O.B.J. Corneth, P.S. Asmawidjaja, A. Mus, E. Lubberts
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> IL-23 is essential in the development of chronic autoimmune diseases and supports the maturation of pathogenic Th17 cells. Although the role of IL-23 during adaptive immunity in arthritis has been studied extensively, the role of IL-23 during acute joint inflammation is unknown. <h3>Objectives</h3> To investigate the role of IL-23 in the development of an acute, macrophage-mediated joint inflammation. <h3>Methods</h3> Peptidoglycan (PG) or streptococcal cell wall fragments (SCW) were intra-articularly injected into the knee joint of naïve wt or IL-23p19 deficient mice. Joint swelling was assessed by measuring joint thickness using a caliper. In addition, synovial expression of different cytokines was measured by specific ELISA and/or Q-PCR. Moreover, synovial explants of wt and IL-23p19 deficient mice were stimulated with SCW and cytokine levels were measured by ELISA. <h3>Results</h3> TLR2/NOD2-mediated streptococcal cell wall (SCW) and peptidoglycan (PG) induced acute joint inflammation in IL-23p19-deficient mice resulted in a profound reduction of local joint inflammation compared to control mice in both these models. Synovial IL-23p19 transcript was detected at 1.5 and 4 hours after arthritis induction and was further increased 1 day after PG injection with a peak at day 2. Interestingly, IL-23p19-/- mice showed a significant reduction in synovial TNF-a, but not IL-6 levels 4 hours after TLR2/NOD2-mediated arthritis induction in the knee joint. In line with this, reduced TNF-a levels were detected in the culture supernatant of SCW-stimulated synovial explants from IL-23p19-/- mice compared to control mice. <h3>Conclusions</h3> These data show a critical role for IL-23 in the development of a TLR2/NOD2-mediated acute joint inflammation and reveal a novel IL-23/TNFalpha axis in this process. <h3>Disclosure of Interest</h3> None Declared