tbuckley/cabot-search huggingface dataset:
Abstract 523: γα T cells are activated by protein-bound polysaccharide krestin (PSK) and contribute to the antitumor effect of PSK
Authors: Carol Inatsuka, Yi Yang, Ekram Gad, Lauren Rastetter, Mary Disis, Hailing Lu
Journal: Cancer Research (2012)

Abstract Gamma delta (γδ) T cells play an important role in anti-tumor immunity, and novel methods to augment γδ T cell function are highly desired. It has recently been shown that toll-like receptor (TLR) agonists may modulate the function of γδ T cells, so we questioned whether protein-bound polysaccharide krestin (PSK), a mushroom extract with TLR2 agonist activity, can activate γδ T cell. Using splenocytes from neu-transgenic mice, we found that γδ T cells produce IFN-γ after PSK treatment and have up-regulated expression of CD25 and CD69. The expression of CD107a in γδ T cells was also enhanced by PSK. To investigate whether the effect of PSK on γδ T cells is direct or indirect via activation of dendritic cells (DC), purified γδ T cells were cultured either alone or together with bone marrow derived DC (BMDC) in a co-culture or trans-well system and then stimulated with PSK. Results showed that direct cell-to-cell contact between γδ T cells and DC is required for optimal activation of γδ T cells. In the absence of DC, PSK can co-stimulate γδ T cells with anti-γδ TCR or anti-CD3 mAb. Oral administration of PSK in neu-transgenic mice with implanted breast tumors resulted in increased percentage of γδ T cells among splenocytes and increased expression of NKG2D on γδ T cells. Depleting γδ T cells during PSK treatment decreased the anti-tumor effect of PSK. All together, these results demonstrated that γδ T cells are activated by PSK and contribute to the anti-tumor effect of PSK. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 523. doi:1538-7445.AM2012-523
AB0167 Autocitrullinated peptidylarginine deiminase from porphyromonas gingivalis part of an infection model in RA?
Authors: M. Sohn, B. Marklein, G.R. Burmester, K. Skriner
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Bacteria release outer membrane vesicles (OMV) which do contain proteins, DNA and enzymes like Peptidylarginine Deiminase from Porphyromonas gingivalis (PPAD) to interact with bacteria and the host immune system. PPAD has been implicated in the pathogenesis of rheumatoid arthritis (RA). <h3>Objectives</h3> We have examined were PPAD its modified in the immune response which patients and controls do respond to Porphyromonas gingivalis outer membrane vesicles (POMV) and PPAD. <h3>Methods</h3> PPAD was analysed in POMVs, recombinantly expressed and separated by 2D SDS-PAGE. PPad was analysed my mass spectrometry (MS). Modifications and the immune reactivity was tested with sera in ELISA and immunobloting. <h3>Results</h3> We have analysed the POMV reactivity and found significant reactions of RA patients and controls to the PPAD and other proteins of the POMV. We could show that PPAD in OMVs is cleaved rendering it into an active enzyme. POMVs can interact with histones to protect bacteria from antibacterial response of histones. Moreover the anti H1 histone antibody response of SLE sera can be blocked by POMVs. H1 histone citrullinated with rabbit PAD was positive with ACPA positive and negative RA and SLE sera. Out of 69 early RA sera, and 23 periodontitis sera (PD), 10 SLE and 10 healthy controls 100% of the early RA and PD sera, 70% of the SLE and 90% of the healthy control sera were reactive with the recombinant PPAD. This immune reactivity correlated with the positivity to P.g LPS ELISA. Specific response to the citrullinated form of PPAD could be detected with affinity purified ACPA antibodies from different citrullinated peptides only with RA sera. The human affintity purified patient ACPA (anti-citrullinated protein antibody) antibodies reacted specifically with the 2D SDS-PAGE separated autocitrullinated form of PPAD. Autocitrullination of PPAD was detected by MS, with human anti Cit-Fibrinogen antibodies and the anti-modified citrulline kit. PPAD citrullination activity was tested with different peptide sequences containing arginine. Sequence and pH dependent cit-reactions could be detected. <h3>Conclusions</h3> PPAD autocitrullination and sequence and pH dependent intramolecular citrullination can be detected using different methods. Anti OMV and PPAD antibody reaction is commonly found in RA, SLE and healthy controls. ACPA specific binding with the autocitrullinated form of PPAD can be only detected in RA sera. The autocitrullinated bacterial protein PPAD might be part of an infection model for the generation of RA specific ACPAs. <h3>Disclosure of Interest</h3> None Declared
P1322 REAL-TIME SHEAR-WAVE ELASTOGRAPHY (SWE, AIXPLORER™) PERFORMANCES IN CHRONIC HEPATITIS C (CHC) PATIENTS COMPARED TO LIVER STIFFNESS MEASUREMENT (LSM, FIBROSCAN™) AND FIBROTEST™ WITH LIVER BIOPSY (LB) AS REFERENCE
Authors: M. Galushko, I. Bakulin, M. Munteanu, V. Kushnir
Journal: Journal of Hepatology (2014)
447 IL28B POLYMORPHISM IS NOT ASSOCIATED WITH RESPONSE TO CONVENTIONAL INTERFERON TREATMENT AND HBSAG SEROCLEARANCE IN HBEAG-POSITIVE CHRONIC HEPATITIS B PATIENTS
Authors: O. Lada, Q. Zhang, M. Lapalus, R. Moucari, S. De Muynck, M. Martinot-Peignoux, I. Bieche, C. Laouenan, E. Estrabaud, N. Boyer, M. Vidaud, T. Asselah, P. Marcellin
Journal: Journal of Hepatology (2012)
Private provider of out of hours care breached targets because of unfilled shifts
Authors: Z. Kmietowicz
Journal: BMJ (2013)

The private provider Harmoni was given until 18 May to spell out how it will increase recruitment to its out of hours service in north London after failing to meet the level of staffing demanded by the health regulator, the Care Quality Commission.1

The out of hours service provided by Harmoni covers 250 general practitioners in the London boroughs of Camden, Islington, Haringey, City, and Hackney—who serve a population of 1.2 million people.

The latest inspections, which took place on 11 and 12 March, followed claims by doctors in December 2012 that the service was so understaffed that it was unsafe.2 The death of a 7 week old baby from a suspected respiratory infection is being investigated after …
Persistence of HIV-1 Transcription in Peripheral-Blood Mononuclear Cells in Patients Receiving Potent Antiretroviral Therapy
Authors: Manohar R. Furtado, Duncan S. Callaway, John P. Phair, Kevin J. Kunstman, Jennifer L. Stanton, Catherine A. Macken, Alan S. Perelson, Steven M. Wolinsky
Journal: New England Journal of Medicine (1999)

Although potent antiretroviral therapy can control infection with human immunodeficiency virus type 1 (HIV-1), a long-lived reservoir of infectious virus persists in CD4+ T cells. We investigated this viral reservoir by measuring the levels of cell-associated viral DNA and messenger RNA (mRNA) that are essential for HIV-1 replication. Approximately every 6 months, we obtained samples of peripheral-blood mononuclear cells from five men with long-standing HIV-1 infection who had had undetectable levels of plasma HIV-1 RNA for 20 months or more during treatment with potent antiretroviral drugs.
Effect of Bradykinin-Receptor Blockade on the Response to Angiotensin-Converting–Enzyme Inhibitor in Normotensive and Hypertensive Subjects
Authors: James V. Gainer, Jason D. Morrow, Angela Loveland, Debbie J. King, Nancy J. Brown
Journal: New England Journal of Medicine (1998)

Angiotensin-converting–enzyme (ACE) inhibitors not only decrease the production of angiotensin II but also decrease the degradation of bradykinin. In this study, a specific bradykinin-receptor antagonist, icatibant acetate (HOE 140), was used to determine the contribution of bradykinin to the short-term effects of ACE inhibition on blood pressure and plasma renin activity in both normotensive and hypertensive subjects.
Sesamoids Opened Earlier and Easier by Bone Imaging
Authors: WONSICK CHOE.
Journal: Clinical Nuclear Medicine (1997)

A 20-year-old woman who was a tennis player underwent three-phase bone imaging to rule out fracture in the right foot. She complained of pain in the region of the first digit, but x-rays did not show any changes. Bone imaging showed increased bilateral uptake in the sesamoids of the great toes on blood pool and delayed images. It was thought that the findings were suggestive of sesamoiditis or stress to the sesamoids.
SAT0407 Association between the rates of enlarged nailfold capillaries and serum FDP-E levels reflects microvascular injuries in juvenile dermatomyositis
Authors: M. Kikuchi, T. Kanetaka, T. Nozawa, T. Kizawa, T. Miyamae, T. Imagawa, S. Yokota
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> One indicator of the active microvascular damages of juvenile dermatomyositis (JDM) is enlarged nailfold capillary (NFC). We have reported that serum FDP–E level is a marker of fibrinogenolysis and reflects maicroangiopathy in JDM patients. <h3>Objectives</h3> To determine clinical factors associated with the rates of enlarged NFCs. <h3>Methods</h3> Forty NFC images of fifteen JDM patients (7.3±3.9 years at first visit) were evaluated. NFC findings of 4 fingers of hand, excluding thumb were recorded in maintaining a constant room temperature of range 24–26°C. The rates of enlarged capillaries (&gt;20 μm) to total number of end raw loops per millimeter in 4 digits were evaluated. Correlations between the rates of enlarged NFCs and serum level of CK, aldorase, FDP-E and ESR were examined. Associations between clinical manifestations (skin involvemrnts and muscle weakness) and the rates of enlarged NFCs were also evaluated. The rates of enlarged NFCs were devided into four groups in this study (group 1=0∼25%, group 2=26∼50%, group 3=51∼75%, group 4=76∼100%), and rates of abnormal levels of serum CK, aldolase, FDP-E, and ESR were evaluated, respectively. <h3>Results</h3> The rates of enlarged NFCs were significantly associated with serum FDP-E levels (r=0.34, P&lt;0.05). Skin involvements were associated with higher rates of enlarged NFCs. Furthermore, abnormal FDP-E levels were found in 33% in group 1, whereas in group 4 abnormal FDP-E levels were found in all patients (p=0.0034). <h3>Conclusions</h3> Enlarged NFCs may reflect microangiopathy in active disease of JDM. Serum FDP-E levels will indicate microvascular inflammation especially in skin involvements. <h3>References</h3> Takayuki K, et al. Clinical analysis of juvenile dermatomyositis; a retrospective study of 45 Japanese. Eular congress. 2009 <h3>Disclosure of Interest</h3> None Declared
Abstract 3711: Human colon mucosal crypts in culture: a model to assess colon chemoprevention.
Authors: Michael K. Dame, Yan Jiang, Danielle Kim Turgeon, Henry Appelman, Muhammad N. Aslam, Kelly Copley, Durga Attili, Dean E. Brenner, James Varani
Journal: Cancer Research (2013)

Abstract Introduction: In order to advance a primary cell culture model of human colonic carcinogenesis we developed methods for the isolation and in vitro maintenance of intact colonic crypts from normal human colon tissue and adenomatous polyps. Methods: Fresh biopsy samples were transported in culture medium containing 1.5mM Ca2+ and antimicrobials. The mucosa/submucosa was surgically separated from the muscularis propria and incubated in 10mM dithiothreitol followed by 8mM ethylenediaminetetraacetic acid. Crypts were agitated free, washed with slow spins, and immediately embedded in a 3-dimensional environment at a density of 50 crypts/50μl Matrigel. After 3 hours, media was replaced to remove non-viable cellular products. Serum-free medium, containing low Ca2+ (0.15mM), was used to maintain crypt cultures. Cell proliferation and differentiation were assessed with markers Ki67 and E-cadherin by confocal microscopy. The fluorescence signal was quantified for the bottom 1/3 of the crypts (35 cells from base on one crypt edge) using morphometric imaging software. In parallel studies, abnormal crypt-like structures were isolated from adenomatous polyps/adenocarcinoma and maintained in a similar culture system. Results: At day-1 in culture, Ki67 expression was strong in the proliferating cells at the crypt base, with E-cadherin staining increasing towards the crypt apex. By day-3, E-cadherin expression became much more prominent and Ki67 decreased dramatically. This culture system preserved the in vivo phenotype of the crypts and allowed for the colonic epithelial cells to follow the physiological sequence of progression from proliferation to differentiation. Adenomatous tissue in culture at day-1 showed expression for Ki67 and E-cadherin but lacked spacial organization. Conclusion: Intact colonic crypts from normal and abnormal human mucosa can be viably maintained in 3-D culture for 72 hours. This primary human in vitro 3-D model may be used to model efficacy of colonic cancer chemopreventives. Epithelial Cell Proliferation and Differentiation in Cultured Human Crypts (fold changes) Markers Zero-time 24 hours 48 hours 72 hours Ki67 1.00 0.36 0.18 0.00 E-cadherin 1.00 2.00 2.89 3.22 Citation Format: Michael K. Dame, Yan Jiang, Danielle Kim Turgeon, Henry Appelman, Muhammad N. Aslam, Kelly Copley, Durga Attili, Dean E. Brenner, James Varani. Human colon mucosal crypts in culture: a model to assess colon chemoprevention. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3711. doi:10.1158/1538-7445.AM2013-3711
AB0690 Bone Mineral Density in Patients with Early Axial Spondyloarthritis
Authors: E. Gubar, A. Bochkova, A. Smirnov, T. Dubinina, A. Demina, O. Rumyantseva, S. Shubin, S. Gluhova, S. Erdes
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Bone loss in patients (pts) with early axial spondyloarthritis is insufficiently studied. <h3>Objectives</h3> To examine bone mineral density (BMD) at the femoral neck (FN) and lumbar spine (LS) in pts with early axial spondyloarthritis (axSpA). <h3>Methods</h3> 73 pts (33 men and 40 women) with early axSpA were included in this study; mean disease duration 19.9±14.4 months. Pts fulfilled ASAS criteria. Mean age 28.3±6.4 years, mean BASDAI 4.1±1.9; mean ASDAS-CRP 2.7±1.3. Clinical data were collected and BMD was measured using dual energy x-ray absorptiometry (DXA) of the FN and LS (L2–4). Low BMD was defined as T score ≤ -1. <h3>Results</h3> In all 73 pts mean T-score was -0.7±0.78 for the FN and -0.9±1.03 for the LS. In the group of pts with bone loss the BMD values were the following: mean T-score for the FN -1.5±0.39 and for the LS -1.6±0.46. Correlation has been detected between BMD reduction in the FN and in the LS (r =0.42; p=0.0001). The reduction in BMD was found in 44 (60.3%) pts. 27 (37.0%) pts had bone loss of the FN, 37 (50.7%) pts had bone loss of the LS, BMD reduction in both areas was found in 20 (27.4%) pts. <h3>Conclusions</h3> Systemic bone loss starts early in the disease process and is revealed in a considerable part of pts with early axSpA. This emphasizes the need for early intervention in axSpA. There is an association between low BMD in the FN and in the LS. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3083
AB0495 Audiovestibular involvement in systemic sclerosis: our exprerince
Authors: E. Lanciano, C. Rotondo, E. Praino, M. Covelli, F. Iannone, G. Lapadula
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Hearing and balance disorders may occur in systemic sclerosis (SSc) as a result of the immune-mediated inner ear damage, the etiology being vasculitis and fibrosis. Objective of our study was to evaluate the prevalence of the audiovestibular organ involvement and its relationship with duration of the disease in patients with SSc. <h3>Methods</h3> In order to evaluate the nature and association of audiovestibular disturbances and systemic sclerosis (SSC), 19 selected SSc patients (2 males and 18 females) were studied with a detailed audiological and vestibular examination. Patients were 15-77 years old and were affected by SSc from around10 years. Patients had no history of exposure to noise, ototoxic drugs, hearing loss metabolic desease-associated and genetic hearing loss. Otoscopy, liminal tonal audiometry, speech audiometry, impedance and evoked otoacoustic emissions was performed. <h3>Results</h3> Nine patients (47%) had normal hearing (PTA &lt; 20 dB HL), while ten patients (53%) had hearing loss (one had severe hearing loss with PTA ≤ 71-90 dB HL, five moderate PTA ≤ 41-70 dB HL and four mild heraring loss with PTA ≤ 20- 40dB HL). Four patients had unilateral and 15 bilateral hearing loss. Nine patients (47%) showed sensorineural hearing loss mostly bilateral and symmetrical, while four patients (21%) had a bilateral transmissive hearing loss and six patients (32%) had normal hearing. <h3>Conclusions</h3> We observed a loss of hearing threshold sensorineural, bilateral and symmetrical, higher in subjects aged 55-64 years with 9-10 years disease duration. SSC appears to be directly responsible for audiovestibular damage, no other apparent cause could be revealed. Thus, screening for audiovestibular involvement should be considered in SSc patient follow-up. <h3>References</h3> Audiovestibular involvemente in Systemic Sclerosis. Berrettini S, Ferri C, Pitaro N, Bruschini P, Latorraca A, Sellari-Franceschini S, Segnini G. ORL J Otorhinolaryngol Relat Spec. 1994 Jul-Aug;56(4):195-8. Vestibular system in patients with systemic sclerosis. 2011 May-Jun;65(3):202-7. doi: 10.1016/S0030-6657(11)70676-X. Polish. Audiovestibular manifestations in patients with limited systemic sclerosis and centromere protein-B (CENP-B) antibodies. Amor-Dorado JC, Arias-Nuñez MC, Miranda-Filloy JA, Gonzalez-Juanatey C, Llorca J, Gonzalez-Gay MA. Medicine (Baltimore). 2008 May;87(3):131-41. <h3>Disclosure of Interest</h3> None Declared
High mortality following gastrostomy tube insertion in adult peritoneal dialysis patients: case report and literature review
Authors: R. Dahlan, M. Biyani, B. McCormick
Journal: Endoscopy (2013)

A 79-year-old woman on peritoneal dialysis was admitted to hospital on January 14, 2013, for management of bilateral nonhealing heel ulcers. During her hospital stay, her oral intake was very poor and her albumin level progressively declined to 8 g/L. On February 15, she underwent percutaneous endoscopic gastrostomy (PEG) tube insertion and was switched to hemodialysis. Prophylactic oral fluconazole and intravenous piperacillin/tazobactam were administered. On February 23, she developed fever, hypotension, and tachycardia, and subsequently had a cardiorespiratory arrest. She was resuscitated and transferred to the intensive care unit. A leak around the PEG tube site was noted and the PD catheter drained turbid fluid with a total white cell count of 9800 × 10–6/L and effluent culture grew Klebsiella oxytoca, Pseudomonas aeruginosa, Enterococcus species, and Candida albicans. Imaging showed no evidence of bowel perforation. The patient continued to deteriorate and she died on February 24, 2013.
Functional Capabilities of Steroid-Recruited Neutrophils Harvested for Clinical Transfusion
Authors: Lewis Glasser, Douglas W. Huestis, James F. Jones
Journal: New England Journal of Medicine (1977)

To determine whether steroids exert a direct inhibitory effect on neutrophil function, thus contraindicating their use to increase granulocyte yields for white-cell transfusions to infected neutropenic patients, we gave normal donors a single intravenous dose of dexamethasone (4 mg per square meter of body-surface area). Approximately two hours later the absolute neutrophil count increased from an initial value (mean ±1 S.D.) of 3800±1400 to 5700±2400 per microliter. Granulocytes were then collected by discontinuous-flow centrifugation and tested. Viability (98.4 per cent), the percentage of neutrophils capable of phagocytosis (97.6), particle accumulation (12.3 candida per phagocyte), fungicidal activity (1.08 "ghost" yeast cells per phagocyte), the percentage of bacteria killed (97.7) and chemotaxis (119 per cent) did not significantly differ from observations in nontreated paired controls. The functional competence of neutrophils used for granulocyte transfusions was not altered after short-term exposure of these healthy donors to steroid medication. (N Engl J Med 297: 1033–1036, 1977)
Abstract 5267: Mouse models of cancer at The Jackson Laboratory Repository
Authors: Deborah M. Boswell, Stephen F. Rockwood, Michael Sasner, Cathleen M. Lutz, Muriel T. Davisson, Leah Rae Donahue
Journal: Cancer Research (2012)

Abstract As one of the largest collections of characterized mouse strains available, The Jackson Laboratory Repository serves the scientific community as a resource to archive and distribute mouse models. Among the hundreds of new strains acquired or developed annually are a transgenic model for prostate hyperplasia that progresses to adenoma/adenocarcinoma, a transgenic model of large B cell lymphoma similar to tumors seen in patients with SLE and Sjogren's syndrome; and a targeted mutation strain of Lats1 (large tumor suppressor) that develops soft tissue sarcomas, ovarian stromal cell tumors and increased sensitivity to carcinogens. Conditional (floxed) strains that are useful in generating tissue-specific mutants have been recently added to the Repository and include mutations of Perp (TP53 apoptosis effector), Msh2 (mutS homolog 2, E. coli), and Irf8 (interferon regulatory factor 8). A transgenic strain that expresses Cre recombinase specifically in the large intestine has applications in colon cancer research. A combination mutant strain (containing targeted mutations of Vhl, von Hippel-Lindau tumor suppressor, and Pten, phosphatase and tensin homolog, and a transgene with Cre recombinase expression driven by Cdh16, cadherin 16) is a model for benign mixed adenosquamous genital tract tumors resembling clear cell cystadenomas observed in patients with Von Hippel-Lindau syndrome. The TEL-AML1 (Etv6-RUNX1, t12;21) translocation/fusion protein associated with childhood acute lymphoblastic leukemia is conditionally expressed by a targeted mutation strain. For transplantation research, a number of “humanized” mouse strains are available. These typically are immune compromised lines and/or lines that provide continuous circulation of human growth factors supporting a platform for a humanized microenvironment. A number of new research tool strains have been added to the Repository, including a fluorescent reporter for carcinoma metastasis, the Cre reporter R26R-Confetti strain which allows individual cell labeling with nuclear-localized membrane-targeted or cytoplasmic fluorescent proteins, and transgenic strains expressing the KikGR flurorescent protein which changes color from green to red upon activation. The Repository maintains a searchable on-line resource (www.jax.org) for each strain. Researchers can submit their strains by using the on-line form available at The Jackson Laboratory website: www.jax.org/donate-a-mouse. Supported by NIH, HHMI, The Ellison Medical Foundation and several private charitable foundations. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5267. doi:1538-7445.AM2012-5267
RISK OF SURGERY AMONG PATIENTS WITH OR WITHOUT PREVIOUS CORONARY DRUG-ELUTING STENT IMPLANTATION
Authors: Gro Egholm, Steen Kristensen, Troels Thim, Kevin K.W. Olesen, Morten Madsen, Henrik T. Sørensen, Svend Eggert Jensen, Lisette O. Jensen, Hans Erik Bøtker, Michael Maeng
Journal: Journal of the American College of Cardiology (2016)