tbuckley/cabot-search huggingface dataset:
FRI0465-HPR Association between one leg stand test, self-reported knee instability and activity limitations in patients with established knee osteoarthritis
Authors: D.C. Sanchez-Ramirez, M. van der Leeden, L.D. Roorda, S. Verschueren, W. Lems, J. Dekker
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> To our knowledge it is still unknown if there is a correlation between an easy to implement balance test such as one leg stand test (OLST), with self-reported knee instability and functional ability in patients with established knee osteoarthritis (OA). <h3>Objectives</h3> To determine the association between one leg stand test (OLST), self-reported knee instability and activity limitations in a cohort of patients with established knee osteoarthritis (OA). <h3>Methods</h3> Data are from 294 patients with established knee OA from the Amsterdam Osteoarthritis cohort. In the OLST, the patient was asked to stand on one leg for 30 seconds without additional support. Activity limitations were assessed using performance test (i.e. Get Up and Go test (GUG), and a stair climbing-descending test) and a questionnaire (Western Ontario and McMaster Osteoarthritis Index-subscale Physical Function (WOMAC-PF). Self-reported knee instability was reported as episodes of buckling, shifting or giving way of the knee in the previous three months. Regression analyses were used to assess the association between OLST and self-reported knee instability and activity limitations. <h3>Results</h3> Completion of OLST was highly associated with less time performing the GUG test (-2.67, p&lt;0.001) and walking up-down stairs (-7.86, p&lt;0.001), and lower WOMAC-PF score (-5.46, p&lt;0.001). The associations were less strong but still significant for the GUG test and time walking up-down stairs after adjustment for BMI, laxity, ROM active flexion, muscle strength and pain. A significant association was observed between OLST and self-reported knee instability (OR=0.57, p=0.038); however it disappeared after adjustment for BMI, ROM active flexion, muscle strength or pain. <h3>Conclusions</h3> It was found that OLST was highly associated with performance-based activity limitations in persons with knee OA, but not with WOMAC-PF score and self reported knee instability when taking into account relevant co-variables. OLST is an easy to implement balance evaluation tool which requires only limited space and time. It may be helpful detecting targets for treatment of patients with knee OA. <h3>Disclosure of Interest</h3> None Declared
Abstract 2624: Evaluation of the association of the ABO blood group SNP rs505922 with breast cancer phenotypes
Authors: Seth K. Rummel, Rachel E. Ellsworth, Craig D. Shriver
Journal: Cancer Research (2012)

Abstract Purpose: To date, evaluation of the association of the ABO blood group and breast cancer has yielded mixed results. SNP rs505922, located within the first intron of the ABO gene, has been associated with the adenocarcinoma subtype of pancreatic cancer. To determine whether the ABO blood group is associated with risk of breast cancer, rs505922 was genotyped in 629 Caucasian women with invasive breast cancer, representing a variety of clinical and pathological tumor types. Methods: Genomic DNA was isolated from blood clots. TaqMan SNP assay C_2253769_10 was used to determine the genotypes for rs505922. Statistical analysis was performed using chi-square analysis using a P-value &amp;lt;0.05 to define significance. Results: Genotypes were generated for 100% of the 629 patients in this study. Allele and genotype frequencies did not vary significantly for age at diagnosis, tumor stage, size or grade, hormone, HER2 or lymph node status, intrinsic subtype, tumor type or patient outcome. Conclusions: Allele frequencies for rs505922 did not differ between women with breast cancer and published HapMap frequencies. Further stratification into different tumor phenotypes also failed to reveal an association between rs505922 and any tumor characteristics. Together, these data suggest that the minor allele of rs505922 and the resulting non-O blood types, are not associated with increased risk or less favorable tumor characteristics or prognosis in breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2624. doi:1538-7445.AM2012-2624
Abstract P3-01-08: Mammographic density and estrogen receptor α gene polymorphism in Javanese ethnic women
Authors: L Choridah, T Aryandono, A Faisal, AH Sadewa, D Purnomosari
Journal: Cancer Research (2012)

Abstract Background: Mammography density reflects the number of stromal and epithelial and one of strongest risk of BC. Estrogen plays an important role in the occurrence of breast cancer through a mechanism of proliferation and genotoxic effects. Action of estrogen on breast tissue by binding to estrogen receptors. The main estrogen receptor α is coded as ESR1 gene. The most studied variants in this gene are the PvuII and XbaI polymorphisms, which have been associated to lower sensitivity to estrogen. Objective: To determine the proportion of genetic variation ESR1 XbaI and PvuII in Javanese ethnic woman in Yogyakarta, Indonesia and to study the correlation between genetic variations in these genes with mammogram density based on quantitative methods. This reaserch to be used as preventive measures for the development of genetic marker breast cancer risk associated with the density of mammography for younger women who have not entered the age to undergo mammography and can be useful as a reference for evaluation of anti estrogen therapy. Method: Conducted digitizer mammogram density assessment using quantitative rating system based on computer-assisted methods of measurement with cumulus program based on interactive thresholding. Polymorphism identification ESR1 XbaI and PvuII RFLP obtained through PCR process. The numbers of genotyped cases and controls for each marker were 50 cases and 58. Mean of density and frequencies of SNPs were compared between cases and controls to identify SNPs associated with cancer susceptibility. Using anova, independent t-test, cruskal wallis and mann whitney u test we calculated significant different between SNP genotype group. Results: The mean of mammographic density is higher in cases (52%) than in controls (0,41%) (p &amp;lt; 0,05). Means of mammographic density were compared by ESR1 genotypes and haplotypes. The percentage density was higher in women with one or two copies of the PvuII p allele (means for CT/Pp and TT/pp are 49 % and 48 %, respectively) than in those with the CC/PP genotype (39%), TT VS CC (P &amp;gt;0,05). Women with one or two copies of the XbaI × allele had higher mean percentage density (AG/Xx and AA/xx, 49% and 47%, respectively) than those with the GG/XX genotype (32%,), AAVGG P &amp;lt; 0,05. Persentage of TT genotype and mean of density is higher in cases (30%, 52%) than controls (10,2%, 40%). Conclusion: Haplotype 2 (TG/PX) was associated with lower sensitivity to estrogen and reflects as decrease of mammographic density. The findings of this study support the view that ESR1 polymorphisms may affect breast cancer risk through differences in breast density. Keywords: Breast Cancer, Mammogram Digitizer, DNA polymorphism ESR 1 PvuII, ESR1 XbaI, PCR RFLP Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P3-01-08.
Abstract 3191: The Akt-mTOR axis determines cell fate through the regulation of eIF2α phosphorylation pathway.
Authors: Clara Tenkerian, Zineb Mounir, Jothi Krishnamoorthy, Antonis E. Koromilas
Journal: Cancer Research (2013)

Abstract Metazoans respond to environmental stress by inducing the phosphorylation of the α subunit of translation initiation factor eIF2 at S51 (eIF2αP), a modification that leads to protein synthesis inhibition. We demonstrate that eIF2αP is induced by pharmacological inhibition or genetic ablation of the PI3K-Akt-mTOR pathway (1). Increased eIF2αP is an evolutionary conserved process that involves the endoplasmic reticulum (ER)-resident protein kinase PERK, which is negatively regulated by Akt dependent phosphorylation at T799. The PERK-eIF2αP arm is downregulated by Akt in cells exposed to ER stress or oxidative stress leading to the induction of cell survival or death respectively. In unstressed cells, the PERK-eIF2αP pathway guards survival and facilitates adaptation to the deleterious effects of PI3K, Akt or mTOR inactivation. Inactivation of the PERK-eIF2αP arm sensitizes tumor death from pharmacological inhibition of the PI3K-Akt-mTOR pathway. The PERK-eIF2αP pathway links Akt and mTOR signaling to translational control with implications in tumor treatment with pharmacological inhibitors of PI3K-Akt-mTOR pathway. Citation Format: Clara Tenkerian, Zineb Mounir, Jothi Krishnamoorthy, Antonis E. Koromilas. The Akt-mTOR axis determines cell fate through the regulation of eIF2α phosphorylation pathway. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3191. doi:10.1158/1538-7445.AM2013-3191 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.
Abstract P4-03-08: A new real-time image fusion technique, a coordinated sonography and MRI using magnetic position tracking system, improves the sonographic identification of enhancing lesions in breast MRI
Authors: S Nakano, K Fujii, M Yoshida, J Kousaka, Y Mouri, T Fukutomi, T Ishiguchi
Journal: Cancer Research (2012)

Abstract Breast magnetic resonance imaging (MRI) is a method that can detect clinically and mammographically occult cancer foci. For lesions suspected to be malignant on the basis of breast MRI, targeted sonography can help determine whether the lesion is amenable to tissue biopsy using sonographic guidance. However, the reproducibility of sonography as well as interexaminer reliability in targeted sonography is still a clinical issue. Recently, we have developed real-time virtual sonography (RVS) that can coordinate a sonographic image and a MRI multiplanar reconstruction (MRI-MPR) of the same section in real time. Although RVS has been reported to be useful for breast cancers, only a few studies have investigated the size and positioning error between a real-time sonographic image and a MRI-MPR image. The purpose of this study was to evaluate the accuracy of RVS to sonographically identify enhancing lesions by breast MRI. Between December 2008 and May 2009, RVS was performed in 51 consecutive patients with 63 enhancing lesions. MRI was performed with the patients in the supine position using a 1.5-T imager with a body surface coil to achieve the same position as with sonography. To assess the accuracy of the RVS, the following three issues were analyzed: (i) The sonographic detection rate of enhancing lesions, (ii) the comparison of the tumor size measured by sonography and the MRI-MPR and (iii) the positioning errors as the distance from the actual sonographic position to the expected MRI position in 3-D. Among the 63 enhancing lesions, 42 (67%) lesions were identified by conventional B-mode, whereas the remaining 21 (33%) initial conventional B-mode occult lesions were identified by RVS alone. The sonographic size of the lesions detected by RVS alone was significantly smaller than that of lesions detected by conventional B-mode (p &amp;lt; 0.001). The mean tumor size provided by RVS was 12.3 mm for real-time sonography and 14.1 mm for MRI-MPR (r = 0.848, p &amp;lt; 0.001). The mean positioning errors for the transverse and sagittal planes and the depth from the skin were 7.7, 6.9 and 2.8 mm, respectively. The overall mean 3D positioning error was 12.0 mm. Our results suggest that RVS has good targeting accuracy to directly compare a sonographic image with MRI results without operator dependence. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P4-03-08.
Fibrin sealant for the prevention of axillary seroma formation after axillary lymph node disection. A prospective randomized study.
Authors: JI Sanchez-Mendez, MJ Lopez-Rodriguez, C Sanchez-Martinez, MT Martinez-Garcia, MD Iniesta Doñate, J Ordas Santotomas
Journal: Cancer Research (2009)

Abstract Abstract #4139 Background&amp;#x2028; The most frequent postoperative complication after axillary limph node dissection is the axilary seroma (AS) formation. Currently accepted practice includes insertion of one or more drainage system for fluid evacuation. Several methods have been proposed to decrease AS formation, such as closed suction catheters, shoulder immobilisation, tetracycline sclerotherapy, talc podrage and closure of dead space; however no of them have reduced the incidence of AS. Seromas are associated with wound infection, wound dehiscence and skip flap necrosis, which prolong the recovery and can extend patient´s stay in Hospital.&amp;#x2028; The aim of this prospective randomized controlled trial was to evaluate the efficacy of fibrin sealant (FS) on the prevention of AS.&amp;#x2028; Material and Methods&amp;#x2028; Sixty-two patients undergoing axillary lymph node dissection were randomized to treatment with drainaje alone (arm A, n=49), drainage plus FS (arm B, n=12), or FS alone (arm C, n=53). Arm B was cancelled after primary results. Efficacy was evaluated in terms of lengh of hospital stay, volumen of fluid drainage and rate of wound related complications (seroma, hematoma, infection, dehiscence) in each arm.&amp;#x2028; Results&amp;#x2028; No significative differences are detected neither type of surgery, nor histlogycal characteristics.&amp;#x2028; &amp;#x2028; The mean result is a difference of almost minus 3 days in postoperative stay in the FS group.&amp;#x2028; &amp;#x2028; Conclusions&amp;#x2028; 1- Application of fibrin sealant reduces the amount of serosanguinous drainage and the length of postoperative stay in patients who have undergone axillary lymph node dissection.&amp;#x2028; 2- Fibrin sealant allows to avoid the insertion of an axillar drainage system&amp;#x2028; 3- It is very important the correct preparation and application of the fibrin sealant and it should be performed by a qualified surgeon.&amp;#x2028; 4- Future clinical trials toasses the role of fibrin sealant seroma are warranted. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 4139.
Abstract 5288: S100PBP is ubiquitously expressed and modulates the function of S100 calcium-binding proteins in pancreatic cancer
Authors: Kate E. Lines, Namaa Audi, Claude Chelala, Hemant Kocher, Nilu Wijesuriya, Helen Hurst, Tatjana Crnogorac-Jurcevic
Journal: Cancer Research (2010)

Abstract We have recently discovered a 37kDa novel S100P-binding protein, S100PBP, which shows no homology to any currently known protein; we have therefore explored its expression in a wide range of normal and cancer tissues, its cellular localization and performed several in vitro functional assays to define its putative roles. In order to determine S100PBP localization in human samples immunohistochemistry was performed on a multi-organ tissue array (Pantomics) and on an in-house made pancreatic ductal adenocarcinoma tissue array including normal, cancer and metastatic lesions. To determine S100PBP function cells with both silenced and stably over-expressing S100PBP were established and investigated by Affymetrix gene expression arrays and a battery of functional assays including adhesion, migration and invasion. We show that this protein is almost ubiquitously expressed, in varying levels, between normal and tumor specimens, which is both cancer- and tissue-type dependent. In normal pancreas it is widely expressed in both exocrine and endocrine compartments while in pancreatic adenocarcinoma its expression is absent in the stroma and retained in some of the cancer cells but with significantly lower intensity. We further show that in addition to S100P, in pancreatic cancer cells S100PBP binds, in a calcium-dependant fashion, several other S100 proteins, namely S100A2, S100A4 and S100A6. Over-expression of S100PBP led to changes in the expression levels of S100P and S100A9, which were down-regulated, and S100A4, which was up-regulated. Furthermore, manipulation of S100PBP expression led to phenotypic changes affecting the adhesive, migratory and invasive capacity of pancreatic cancer cells, that varied depending on the cellular context and constellation of the different S100 proteins present. Both over-expression and silencing of S100PBP also led to an increase/decrease in Cathepsin Z levels, respectively. In summary, S100PBP is ubiquitously expressed and is deregulated in many different cancer types, and appears to play a role in the adhesive, migratory and invasive capabilities of pancreatic cancer cells. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5288.
International Health Care Systems
Authors: Stephen Morrissey, David Blumenthal, Robin Osborn, Gregory D. Curfman, Debra Malina
Journal: New England Journal of Medicine (2014)

All countries want to meet the health and medical needs of their populations, preferably without bankrupting themselves or draining resources that serve other important human needs and purposes. In the face of varied economic, political, cultural, environmental, epidemiologic, and demographic forces, each country tries to tailor its health care system to the specific characteristics and needs of its population. Yet the building blocks are largely the same: every country requires a basic public health infrastructure, which in developing countries may rely heavily on community health workers. Developed countries require physicians, nurses, and other health care professionals, hospitals and clinics, and . . .
Abstract 2527: Amonafide and its metabolite N-acetyl amonafide are Top2 poisons with differing biochemical properties
Authors: Eroica Soans, Karthik Shanmuganatham, Anna Rogojina, John L. Nitiss
Journal: Cancer Research (2011)

Abstract Naphthalimides derivatives have been extensively studied as anti-cancer agents. Amonafide (5-amino-2-[2-(dimethylamino)ethyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione) showed clinical activity especially in some groups of AML patients. Amonafide has been shown to target topoisomerase II (Top2), and to stabilize Top2 covalent complexes. Therefore, the agent is a Top2 poison, however, the compound shows some unusual properties. Unlike most Top2 poisons, the action of amonafide against Top2 is largely ATP independent; In addition, amonafide leads to cleavage of DNA at a very restricted set of sites compared to other Top2 poisons such as mitoxantrone or etoposide. These findings have led to suggestions that amonafide may target Top2 in an unconventional way. Another obstacle to the clinical use of amonafide is variable drug metabolism. Felder and colleagues showed that amonafide is metabolized by N-acetyl transferase 2 (NAT2) to form N-acetyl amonafide (NAA). Toxicity of amonafide regimens is associated with higher levels of NAT2 activity 2. The mechanism of NAA toxicity has not been reported. We have used the ICE assay developed by Muller and colleagues to assess Top2 covalent complex levels in cells treated with either amonafide or NAA. We found that amonafide induces Top2 mediated DNA cleavage, and that covalent complexes formed by both Top2 alpha and Top2 beta were seen. Interestingly, NAA induced higher levels of Top2 covalent complexes than the parent compound. In addition, the level of Top2 covalent complexes increased with increasing NAA dose, whereas a plateau in the level of Top2 covalent complexes was seen with amonafide at relatively low doses. We are currently comparing the action of amonafide and NAA against purified human topoisomerases. We suggest that NAA is a Top2 poison, and a plausible hypothesis is that NAA may act more like a conventional Top2 poison, while amonafide may show a better therapeutic index because it has more limited potential for forming Top2 covalent complexes. These results may be useful in the further development of amonafide derivatives with a favorable therapeutic profile. (1) Felder, T. B.; McLean, M. A.; Vestal, M. L.; Lu, K.; Farquhar, D.; Legha, S. S.; Shah, R.; Newman, R. A. Drug Metab Dispos 1987, 15, 773. (2) Ratain, M. J.; Rosner, G.; Allen, S. L.; Costanza, M.; Van Echo, D. A.; Henderson, I. C.; Schilsky, R. L. J Clin Oncol 1995, 13, 741. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2527. doi:10.1158/1538-7445.AM2011-2527
IgE antibodies, FcepsilonRIalpha, and IgE-mediated local anaphylaxis can limit snake venom toxicity.
Authors: Philipp Starkl, Thomas Marichal, Nicolas Gaudenzio, Laurent Lionel Reber, Riccardo Sibilano, Mindy Tsai, Stephen Joseph Galli
Journal: Journal of Allergy and Clinical Immunology (2015)
Abstract 450: Host-tumor immune response for breast cancer patients.
Authors: Shigeru Imoto, Noriko Nakatsugawa, Hirotsugu Isaka, Hiroki Ito, Kentaro Imi, Kaisuke Miyamoto, Tetsuya Nakatsura
Journal: Cancer Research (2013)

Abstract We demonstrated immune suppression of regulatory T cells (Treg) and M2 macrophage in breast cancer (BC) patients at AACR 2011. In summary, to compare between 38 early and 12 advanced BC cases, CD163-positive tumor cells and CCR4-positive tumor cells were detected more frequently in advanced cases than in early cases. Treg cells in PBMC significantly increased in percentage of the population in 37 BC patients than in 21 healthy volunteers. To explore host-tumor immune response, several cytokines were examined in the same cases. Plasma from 32 early and 9 advanced BC cases was preoperatively obtained. Human IL-2, IL-4, IL-6, IL-10, TNF, INFγ, and IL-17A were measured using cytometric beads array system. Statistical significance was analyzed using Mann-Whitney U-test. Plasma IL-17A had significantly higher levels in early BC than in advanced BC (103 pg/ml v.s. 51 pg/ml at mean level). Th17 cells may have some potential to control tumor progression in BC. In conclusions, induction of host-tumor immune response due to chemotherapy and anti-HER2 therapy and release from immune suppression such as CCR4 monoclonal antibody are important issues to explore a new strategy for BC patients. Citation Format: Shigeru Imoto, Noriko Nakatsugawa, Hirotsugu Isaka, Hiroki Ito, Kentaro Imi, Kaisuke Miyamoto, Tetsuya Nakatsura. Host-tumor immune response for breast cancer patients. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 450. doi:10.1158/1538-7445.AM2013-450
830 CHOLESTEROL LEVELS AND PRESENCE OF DIABETES PREDICT EARLY VIROLOGICAL RESPONSE TO TRIPLE THERAPY WITH TELAPREVIR, PEG-INTERFERON alfa-2a 180 μg AND RIBAVIRIN IN CHRONIC HEPATITIS C
Authors: E. Jaeckel, E. Zehnter, C. John, R. Heyne, G. Teuber, W. Schiffelholz, S. Christensen, C. Antoni, S. Pape, M. Roessle, H. Loehr, S. Stoll, U. Alshuth, D. Hueppe, S. Mauss, M.P. Manns
Journal: Journal of Hepatology (2013)