tbuckley/cabot-search huggingface dataset:
Magnesium incorporated chitosan based scaffolds for tissue engineering applications
Authors: Udhab Adhikari, Nava P. Rijal, Shalil Khanal, Devdas Pai, Jagannathan Sankar, Narayan Bhattarai
Journal: Bioactive Materials (2016)

Chitosan based porous scaffolds are of great interest in biomedical applications especially in tissue engineering because of their excellent biocompatibility in vivo, controllable degradation rate and tailorable mechanical properties. This paper presents a study of the fabrication and characterization of bioactive scaffolds made of chitosan (CS), carboxymethyl chitosan (CMC) and magnesium gluconate (MgG). Scaffolds were fabricated by subsequent freezing-induced phase separation and lyophilization of polyelectrolyte complexes of CS, CMC and MgG. The scaffolds possess uniform porosity with highly interconnected pores of 50-250 μm size range. Compressive strengths up to 400 kPa, and elastic moduli up to 5 MPa were obtained. The scaffolds were found to remain intact, retaining their original three-dimensional frameworks while testing in in-vitro conditions. These scaffolds exhibited no cytotoxicity to 3T3 fibroblast and osteoblast cells. These observations demonstrate the efficacy of this new approach to preparing scaffold materials suitable for tissue engineering applications.
Donor Human Milk for Very Low-Birth-Weight Infants
Authors: Tarah T. Colaizy
Journal: JAMA (2016)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
SAT0003 The Role of 12/15-Lipoxygenase in The Pathogenesis of Osteoporosis in Mice
Authors: E. Jeziernicka, P. Bielecki, E. Sacharzewska, J. Niklinski, K. Kowal, O. Kowal-Bielecka
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> The pathogenesis of osteoporosis is complex and involves age-related factors and inflammation. 12/15-lipoxygenase (12/15-LOX) plays a key role in the regulation of inflammatory response and has been shown to contribute in bone metabolisms. However, the exact role of 12/15-LOX in the pathogenesis of osteoporosis is not clear. <h3>Objectives</h3> We aimed to investigate the impact of genetic deletion of <i>12/15-LOX</i> on 1) bone mineral density (BMD) and 2) serum balance between osteoprotegerin (OPG) and RANK ligand (RANKL), the two proteins known to be involved in bone remodelling, in mice. <h3>Methods</h3> The whole body BMD was measured using dual-energy X-ray absorptiometry (DEXA) bone densitometer equipped with software for evaluation of small animals (Hologic Discovery, USA) in 40-weeks old 12/15-LOX knockout mice and their age- and sex-matched wild-type littermates (C57Bl mice). Serum concentrations of OPG and RANKL were measured using commercially available ELISA kits (R&amp;D Systems and USCN Life Science Inc., respectively). <h3>Results</h3> The mean whole body BMD was significantly lower in female 12/15-LOX knockout mice as compared with age-matched female C57Bl mice (p&lt;0.01). No significant differences were found in the mean whole body BMD of male 12/15-LOX knockout mice as compared with their age-matched male wild-type littermates. No significant difference could be found in the mean serum concentration of OPG between 40-weeks old female 12/15-LOX knockout and C57Bl mice. However, in younger (8-weeks old) female C57Bl mice the mean serum concentration of OPG was significantly higher as compared with female 12/15-LOX knockout mice of the same age as well as compared with female 40-weeks old C57Bl or 12/15-LOX knockout animals (p&lt;0.01 for all comparisons). There were no significant differences in the mean serum concentration of RANKL between female 12/15-LOX knockout mice and their wildtype littermates. <h3>Conclusions</h3> The results of our study indicate that 12/15-LOX plays a role in age-related osteoporosis in female mice, possibly through regulation of OPG-RANKL balance. Further studies should address the role of hormonal status on 12/15-LOX interactions with OPG-RANKL pathway. <h3>Disclosure of Interest</h3> None declared
Left Ventricular Cardiac Function during the Course of a One Year Multicenter Trial of the Safety and Efficacy with ICL670 5–40mg/kg/day and Deferoxamine 20–60 mg/kg/day in Beta-Thalassemia Patients with Transfusional Hemosideosis.
Authors: Dorothy A. Kleinert, Erica Jones, Cristina P. Sison, Peter Marks, Patricia J. Giardina
Journal: Blood (2005)
Association Between Allergen Exposure in Inner-City Schools and Asthma Morbidity Among Students
Authors: William J. Sheehan, Perdita Permaul, Carter R. Petty, Brent A. Coull, Sachin N. Baxi, Jonathan M. Gaffin, Peggy S. Lai, Diane R. Gold, Wanda Phipatanakul
Journal: JAMA Pediatrics (2016)

<h3>Importance</h3> Home aeroallergen exposure is associated with increased asthma morbidity in children, yet little is known about the contribution of school aeroallergen exposures to such morbidity. <h3>Objective</h3> To evaluate the effect of school-specific aeroallergen exposures on asthma morbidity among students, adjusting for home exposures. <h3>Design, Setting, and Participants</h3> The School Inner-City Asthma Study was a prospective cohort study evaluating 284 students aged 4 to 13 years with asthma who were enrolled from 37 inner-city elementary schools in the northeastern United States between March 1, 2008, and August 31, 2013. Enrolled students underwent baseline clinical evaluations before the school year started and were then observed clinically for 1 year. During that same school year, classroom and home dust samples linked to the students were collected and analyzed for common indoor aeroallergens. Associations between school aeroallergen exposure and asthma outcomes during the school year were assessed, adjusting for home exposures. <h3>Exposures</h3> Indoor aeroallergens, including rat, mouse, cockroach, cat, dog, and dust mites, measured in dust samples collected from inner-city schools. <h3>Main Outcomes and Measures</h3> The primary outcome was maximum days in the past 2 weeks with asthma symptoms. Secondary outcomes included well-established markers of asthma morbidity, including asthma-associated health care use and lung function, measured by forced expiratory volume in 1 second. <h3>Results</h3> Among 284 students (median age, 8 years [interquartile range, 6-9 years]; 148 boys and 136 girls), exposure to mouse allergen was detected in 441 (99.5%) of 443 school dust samples, cat allergen in 420 samples (94.8%), and dog allergen in 366 samples (82.6%). Levels of mouse allergen in schools were significantly higher than in students’ homes (median settled dust level, 0.90 vs 0.14 µg/g;<i>P</i> &lt; .001). Exposure to higher levels of mouse allergen in school (comparing 75th with 25th percentile) was associated with increased odds of having an asthma symptom day (odds ratio, 1.27; 95% CI, 1.05-1.54;<i>P</i> = .02) and 4.0 percentage points lower predicted forced expiratory volume in 1 second (95% CI, –6.6 to –1.5;<i>P</i> = .002). This effect was independent of allergic sensitization. None of the other indoor aeroallergens were associated with worsening asthma outcomes. <h3>Conclusions and Relevance</h3> In this study of inner-city students with asthma, exposure to mouse allergen in schools was associated with increased asthma symptoms and decreased lung function. These findings demonstrate that the school environment is an important contributor to childhood asthma morbidity. Future school-based environmental interventions may be beneficial for this important public health problem.
FRI0242 Role of Mitochondria- and Nadph Oxidase-Derived ROS in Fibroblasts Isolated from Patients Affected by Systemic Sclerosis
Authors: T. Spadoni, S. Svegliati, A. Gabrielli
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Oxidative stress plays an important role in the development of fibrosis under various pathological conditions. Fibroblasts isolated from patients with Systemic Sclerosis (SSc) overproduce reactive oxygen species (ROS), overexpress type I collagen and α-smooth muscle actin (α-SMA) and show DNA damage and activation of checkpoint kinase ATM (1, 2). NADPH oxidase (NOX) is an important source of ROS, and its overactivity or overexpression are often associated with chronic diseases, characterized by tissue damage and fibrosis. Recently, we have demonstrated that NOX2 and NOX4 are critical components of NADPH oxidase complex in SSc fibroblasts and ROS generated by NOX play a primary role in the pathological activation of dermal fibroblasts (3). Since a recent study reported that NOX4 localizes to membranes and mitochondria and contributes to the generation of mitochondrial ROS (4), we wondered whether mitochondria also play a role in ROS production in SSc fibroblasts. <h3>Objectives</h3> The aim of this study was to investigate the contribution of mitochondrial ROS to oxidative stress in SSc. <h3>Methods</h3> ROS production was analysed by confocal microscopy using dihydroethidium (DHE, to assess all intracellular superoxide), or MitoSOX™ Red (to detect mitochondria superoxide), or using a microplate reader following Amplex® Red kit protocol (to measure H<sub>2</sub>O<sub>2</sub>). Mitochondria were purified using a mitochondria isolation kit. For protein expression, mitochondrial and cytosolic fractions were subjected to western blot with specific antibodies. <h3>Results</h3> SSc fibroblasts incubated with the mitochondria-targeted antioxidant MitoQ showed a reduced MitoSOX™ Red staining, and a partially decreased DHE fluorescence, suggesting that mitochondria contribute to the redox state in SSc fibroblasts. Mitochondria purified from SSc fibroblasts generated significantly higher levels of ROS compared to controls. Incubation of normal cells with PDGF, a profibrotic cytokine able to activate a SSc-like phenotype, led to a significant increase of total and mitochondrial ROS levels compared to unstimulated cells. Mitochondrial and cytosolic fractions from SSc and activated normal fibroblasts were also analyzed to evaluate different protein expression patterns. <h3>Conclusions</h3> In this study we demonstrated that mitochondria contribute to the abnormal redox state of SSc fibroblasts and activated normal cells. Further studies may clarify whether mitochondrial ROS are generated by a mitochondrial NOX isoform or are the result of the interplay between mitochondria and NOX enzymes located outside the organelles. <h3>References</h3> Baroni S et al, N Engl J Med 2006; 354:2667–76; Svegliati S et al, Science Signaling 2014; 7: ra84; Spadoni T et al, Arthritis Rheum 2015; 67:1611–1622; Lee DY et al, J Biol Chem 2013;288:28668–28686. <h3>Disclosure of Interest</h3> None declared
AB1126-HPR How Physically Active Are Nordic Patients with Moderate To Severe Osteoarthritis?
Authors: R.H. Moe, T. Uhlig
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Osteoarthritis (OA) is one of the most frequent chronic rheumatic and musculoskeletal diseases (RMD), and one of the leading causes of pain and disability worldwide. Physical activity is a key lifestyle advice along with weight reduction if overweight. However, data on the amount of physical activity in patients with OA are limited. <h3>Objectives</h3> To examine physical activity in people referred to specialist care for their OA. <h3>Methods</h3> Patients with known OA with moderate to severe OA referred to a specialist clinic in Oslo, Norway were after informed consent mailed questionnaires on demographic data and physical activity before visiting the clinic. Of 391 people, 86% (n=338) were females with mean age (SD) 61.2 (7.8) years). Patients rated their weekly physical activity that included shortness of breath, based on experiences from the last two weeks. Other patient reported outcomes included pain, fatigue and stiffness on numeric rating scales, and body mass index (BMI). Through descriptive statistics and explorative analyses the group was described. Patients who fulfilled 3 hours of physical activity per week or more were slightly leaner and had a numerically lower BMI than those who were less physically active (non-significant differences, data not shown) than those who were less physically active. <h3>Results</h3> See tables. <h3>Conclusions</h3> 29.9% (N=117/391) of OA patients fulfilled the recommended minimum of 3 hours of physical activity per week, while almost 14% reported not being physically active at all. To enhance recommended physical activity, emphasis on physical activity in OA is required during clinical care as well as easy access to programmes for health-enhancing physical activity. These interventions may decrease the burden of OA in the future. <h3>Disclosure of Interest</h3> None declared
OC-068 Measuring the Effect of Ispaghula on Gut Content and Function Using MRI
Authors: G Major, K Murray, A Nowak, C Hoad, C Kurtz, A Silos-Santiago, J Johnston, L Marciani, P Gowland, R Spiller
Journal: Gut (2016)

<h3>Introduction</h3> Ispaghula husk (IS) is believed to modulate functional gastrointestinal symptoms by drawing water into the bowel to soften stool and accelerate transit, and by adding bulk. It is not thought to be readily fermented. Magnetic Resonance Imaging (MRI) can assess gastrointestinal content and function. The aim of the study was to assess whether MRI could detect and quantify the effects of IS in patients with chronic constipation. <h3>Methods</h3> A double-blind crossover study in adults with functional constipation or constipation-predominant irritable bowel syndrome. Intervention: Metamucil Original Coarse Fibre® (P&amp;G, USA) 14 g tds – daily IS dose 21 g. Placebo: Maltodextrin(MD) 14 g tds. On day 5 subjects swallowed 5 gadolinium filled capsules. On day 6 MRI scans were taken fasting and hourly for 7 hours while subjects ingested a rice pudding meal and treatment (IS or MD). Whole gut transit was assessed by the weighted average position score of the capsules 24 hours after ingestion (WAPS). Free water in the small bowel (SBWC) and ascending colon (ACWC) was measured as were T<sub>1</sub> and T<sub>2 </sub>relaxation times in the ascending and descending colon (AC &amp; DC), colonic volume and gas. <h3>Results</h3> 16 subjects completed both treatments. Transit was faster after IS with a mean decrease in WAPS of 24% (p = 0.05, 1 tailed). Postprandial SBWC was markedly higher on IS (p &lt; 0.001) with smaller increases seen in ACWC (p &lt; 0.05). Fasting T<sub>1</sub> was significantly higher after IS in both AC and DC. T<sub>2</sub> fasting values were also higher. A postprandial rise was seen in both T<sub>1</sub>AC and T<sub>2</sub>AC after IS but not MD. Fasting colonic volume increased on PS by mean 332 mL or 48%. Exploratory analysis of colonic gas found that after IS significantly more was detectable both fasting (p &lt; 0.05) and postprandially (p &lt; 0.05). AC gas did not increase until 240 min after PS while transverse colon gas increased steadily through the day. <h3>Conclusion</h3> MRI parameters demonstrated accelerated in transit, increased intestinal water content and increasedcolonic volume with IS. Fasting T<sub>1</sub> appears to discriminate constipation from health and responded to treatment. More colonic gas was detected with IS. This may reflect fermentation of IS or interference with small bowel absorption leading to malabsorption of carbohydrate in the rice meal. These novel findings illustrate the potential for MRI to provide insights into the <i>in vivo</i> effects and mechanisms of action of gut modulators. <h3>Disclosure of Interest</h3> None Declared
Profiling Clinical Platelet and Plasma Use To Inform Blood Supply and Contingency Clanning: Puppy, The Prospective Utilization Of Platelets and Plasma Study
Authors: Mark N. Polizzotto, George Grigoriadis, Neil Waters, Mary Comande, Marija Borosak, Zoe K. McQuilten, Erica M. Wood
Journal: Blood (2013)
KCa3.1 Blockers Inhibit Cell Proliferation in Chronic Lymphocytic Leukemia
Authors: Eva M Groessinger, Lukas Weiss, Elisabeth Hinterseer, Judith Schmoelzer, Karin Oberascher, Daniela Asslaber, Alexander Egle, Tanja Nicole Hartmann, Richard Greil, Hubert H Kerschbaum
Journal: Blood (2012)
Impact of Cytogenetics on Outcomes of Transplant-Ineligible Patients with Newly Diagnosed Multiple Myeloma Treated with Continuous Lenalidomide Plus Low-Dose Dexamethasone in the First (MM-020) Trial
Authors: Herve Avet-Loiseau, Cyrille Hulin, Lofti Benboubker, Meletios A. Dimopoulos, Andrew Belch, Donna Reece, John Catalano, Antonio Pinto, Heinz Ludwig, Nizar J Bahlis, Michele Cavo, Michel Attal, Philippe Moreau, Lugui Qiu, Richard LeBlanc, Rik Schots, S. Vincent Rajkumar, Jennifer Marek, Guang Chen, Wai Yiu, Annette Ervin-Haynes, Thierry Facon
Journal: Blood (2015)
Pooled RCTs: CABG reduces mortality more than PCI with DES in nondiabetic patients with multivessel CAD
Authors: Jennifer A. Rymer, L. Kristin Newby
Journal: Annals of Internal Medicine (2016)

ACP Journal Club15 November 2016Pooled RCTs: CABG reduces mortality more than PCI with DES in nondiabetic patients with multivessel CADJennifer A. Rymer, MD, MBA, L. Kristin Newby, MD, MHSJennifer A. Rymer, MD, MBADuke University Medical Center, Durham, North Carolina, USA (J.A.R., L.K.N.)Search for more papers by this author, L. Kristin Newby, MD, MHSDuke University Medical Center, Durham, North Carolina, USA (J.A.R., L.K.N.)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/ACPJC-2016-165-10-051 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Source CitationChang M, Ahn JM, Lee CW, et al. Long-term mortality after coronary revascularization in nondiabetic patients with multivessel disease. J Am Coll Cardiol. 2016;68:29-36. https://pubmed.ncbi.nlm.nih.gov/27364047Clinical Impact RatingsGIM/FP/GP: Cardiology: References1 Levine GN, Bates ER, Blankenship JC, et al. 2011 ACCF/AHA/SCAI Guideline for percutaneous coronary intervention. A report of the American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines and the Society for Cardiovascular Angiography and Interventions. J Am Coll Cardiol. 2011;58:e44-122. [PMID: 22070834] Google Scholar2 Serruys PW, Morice MC, Kappetein AP, et al; SYNTAX Investigators. Percutaneous coronary intervention versus coronary-artery bypass grafting for severe coronary artery disease. N Engl J Med. 2009;360:961-72. [PMID: 19228612] Google Scholar3 Park SJ, Ahn JM, Kim YH, et al; BEST Trial Investigators. Trial of everolimus-eluting stents or bypass surgery for coronary disease. N Engl J Med. 2015;372:1204-12. [PMID: 25774645] Google Scholar4 Wallentin L, Becker RC, Budaj A, et al; PLATO Investigators. Ticagrelor versus clopidogrel in patients with acute coronary syndromes. N Engl J Med. 2009;361:1045-57. [PMID: 19717846] Google Scholar5 Stone GW, Rizvi A, Newman W, et al; SPIRIT IV Investigators. Everolimus-eluting versus paclitaxel-eluting stents in coronary artery disease. N Engl J Med. 2010;362:1663-74. [PMID: 20445180] Google Scholar Author, Article, and Disclosure InformationAffiliations: Duke University Medical Center, Durham, North Carolina, USA (J.A.R., L.K.N.)This article was published at Annals.org on 1 November 2016. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 November 2016Volume 165, Issue 10Page: JC51KeywordsCardiac surgeryCoronary artery bypass graftingDrugsMortalityMyocardial infarctionPercutaneous coronary interventionRevascularizationStable coronary artery diseaseStent implantationSurgery ePublished: 15 November 2016 Issue Published: 15 November 2016 Copyright & PermissionsCopyright © 2016 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
SFRP1 Is Estrogen Inducible in Bone Marrow Stromal Cells and Suppresses the Earliest Events in Lymphopoiesis.
Authors: Takafumi Yokota, Kenji Oritani, Karla P. Garrett, Taku Kouro, Makoto Nishida, Isao Takahashi, Michiko Ichii, Itaru Matsumura, Paul W. Kincade, Yuzuru Kanakura
Journal: Blood (2007)
A Predictive Cost-Effectiveness Analysis of the Treatment of Chronic, Refractory Immune Thrombocytopenic Purpura (ITP) with Novel Agents: An Economic Model Based on Long-Term Treatment Data with Rituximab Versus Romiplostim.
Authors: Wendy Oatis, Veena Shankaran, Faisal Saghir, Charles L. Bennett, Elizabeth Richey, James B Bussel
Journal: Blood (2008)
Deregulation of Retinoic Inducible Gene---Rig-I Underlies the Pathogenesis of Acute Promyelocytic Leukemia.
Authors: Jiang Zhu, Shu-Hong Shen, Li-Juan Chen, Qiu-Hua Huang, Nan-Nan Zhang, Yi-Wu Sun, Stephen G. Emerson, Sai-Juan Chen, Zhu-Gang Wang, Zhu Chen
Journal: Blood (2005)
Longitudinal Genome Wide Analysis of Patients with Chronic Lymphocytic Leukemia Reveals Complex Evolution of Clonal Architecture At Disease Progression and At the Time of Relapse
Authors: Esteban Braggio, Neil Kay, Scott Van Wier, Renee C. Tschumper, Stephanie A. Smoley, Jeanette E. Eckel-Passow, Traci Sassoon, Daniel L. Van Dyke, John C. Byrd, Diane F. Jelinek, Tait D. Shanafelt, Rafael Fonseca
Journal: Blood (2011)