tbuckley/cabot-search huggingface dataset:
Abstract 2718: Spheroid culture of colorectal cancer cells from patient tumor tissue preserves important cellular characteristics of the parental tumor.
Authors: Maria Jeppesen, Grith Hagel, Anders Glenthoj, Ole Thastrup, Lars Nannestad Jorgensen, Jacob Thastrup
Journal: Cancer Research (2013)

Abstract At the time of diagnosis more than 65% of patients with colorectal cancer have advanced disease and require chemotherapeutic treatment. However, colorectal tumors demonstrate pronounced heterogeneity, leading to significant individual variation in sensitivity to standard chemotherapeutic drugs. Tools for assessing chemosensitivity and tailoring therapeutic regimens to the individual patient are therefore urgently needed. A promising approach is to isolate viable cancer cells from patient tissue and to test the chemosensitivity of the individual tumor in vitro. The aim of the current study was to establish primary colorectal cancer cell cultures from patient tissue and investigate if the cultured cells retain important characteristics of the original tumor. Small cell clusters were isolated from colorectal tumor tissue and allowed to form cell spheroids in suspension culture. The cell type composition of patient spheroids was characterized by immunostaining with cell type specific antibodies. Spheroid size was monitored over time and growth curves established for each patient. The distribution of proliferating and apoptotic cells in the spheroids was assessed by staining for specific markers. The 3D culture system was verified as a tumor model by comparing the results obtained for the spheroids to paraffin-embedded sections of the original tumors. Successful spheroid culture was obtained from more than 80% of tissue samples. Immunostaining confirmed that the primary cultures were composed of colorectal cancer cells, without significant contaminations by other cell types. Growth could be observed as an increase in spheroid size. The dominating cell populations of the original tumor could generally be identified in the derived spheroids as well. In summary, spheroid cultures of colorectal cancer cells can be established from tumor tissue of individual patients with a high success rate and the formed spheroids retain important cellular characteristics of the original tumor. Culture of colorectal cancer cells as spheroids represents a promising method for establishing primary cultures for chemotherapeutic screening of the individual patient. Citation Format: Maria Jeppesen, Grith Hagel, Anders Glenthoj, Ole Thastrup, Lars Nannestad Jorgensen, Jacob Thastrup. Spheroid culture of colorectal cancer cells from patient tumor tissue preserves important cellular characteristics of the parental tumor. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2718. doi:10.1158/1538-7445.AM2013-2718
AB0712 The influence of hand angulation on hand bone mineral density estimates using digital x-ray radiogrammetry
Authors: A. Pfeil, J. Boettcher, R. Froeber, J. Sommerfeld, P. Oelzner, G. Wolf
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Digital X-ray radiogrammetry (DXR) is a computer-assisted technique to quantify cortical hand bone mineral density at the metacarpal bones in patients with rheumatoid arthritis. <h3>Objectives</h3> The aim of this experimental study was to evaluate the influence of rotation of the metacarpal bones on measurements of bone mineral density (BMD) and the Metacarpal Index (MCI) using the DXR technique. <h3>Methods</h3> Hand radiographs of a deceased male were performed at different angulations (between 0 to 19°). Bone mineral density and the Metacarpal Index of the hand were measured by DXR (Version 2, Sectra, Sweden) and the coefficients of variation were measured to verify the reliability and reproducibility of this radiogeometric technique. <h3>Results</h3> Between 0 to 19° no significant changes of the coefficient of variation for bone mineral density and the Metacarpal Index were observed. <h3>Conclusions</h3> The study revealed no significant influence of hand rotation on the measurements of metacarpal bone mineral density as estimated by DXR, indicating the capability of this technique to accurately quantify periarticular cortical bone mass. This is important especially in the field of rheumatoid arthritis as x-ray imaging involves intricate positioning of arthritic hands. <h3>Disclosure of Interest</h3> None Declared
Immunity against poliomyelitis in the Netherlands, assessed in 2006 to 2007: the importance of completing a vaccination series
Authors: N A van der Maas, L Mollema, G A Berbers, D M van Rooijen, H G van der Avoort, M A E Conyn-Van Spaendonck, H E de Melker, F R van der Klis
Journal: Eurosurveillance (2014)

Binary file ES_Abstracts_Final_ECDC.txt matches
Abstract P5-04-08: Identifying and characterizing human kinases for novel regulators of epithelial-mesenchymal transition in breast cancer cells
Authors: L Li, N Azizian, W Li
Journal: Cancer Research (2012)

Abstract The epithelial-mesenchymal transition (EMT) confers mesenchymal properties on epithelial cells and has been closely associated with the acquisition of aggressive traits by breast cancer cells. Recently, a few kinases, such as FYN and c-Met, were reported to participate in regulating EMT. However, the roles of the majority of ∼700 human kinases in EMT and breast cancer progression are still unknown. Here we carried out human kinase cDNA screens to identify novel regulators of EMT. Many unknown EMT regulators and a few known ones such as PI3K, PDGFRA, FYN and c-Met, were found to up-regulate mesenchymal marker vimentin. Some also increase fibronectin and N-cadherin expression in kinase-transducted human mammary gland epithelial cells (HMLER). At the same time, these HMLER cells acquired mesenchymal phenotypes, such as increased in vitro migration activity, enhanced capability to survive and proliferate in growth factor deprivation condition or under drug treatments. RT-PCR results also exhibited increased expression of some EMT transcriptional regulators, such as ZEB1, Snail and Slug, which suggested our selected kinases may regulate EMT through these transcription factors. Interestingly, FACS analysis also showed a substantial increase in CD44hiCD24− population in these kinase-transducted HMLER cells. The increase of CD44hiCD24− mesenchymal phenotype could be attributed to increase of standard form CD44 (CD44s) and decrease of the variant form CD44 (CD44v8-10). And this alternation was associated with down-regulation of ESRP1 and ESRP2, two splicing factors that regulate CD44 splicing and EMT. Therefore, these selected kinases may be considered as novel regulators of EMT, and may aid the development of new prognostic markers or drug targets for breast cancer progression. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P5-04-08.
RADIOIMMUNOIMAGING OF TUMORS WITH INDIUM-111 LABELED F(abʼ)2 ANTI-CARCINOEMBRYONIC ANTIGEN MONOCLONAL ANTIBODY.
Authors: Walter Haindl, Samuel E. Halpern, Robert O. Dillman, Phillip L. Hagan, Jacquelyn Beauregard, Maureen L. Clutter, Diane G. Amox, Kevin Ryan, Richard M. Bartholomew, James M. Frincke, Dennis J. Carlo
Journal: Clinical Nuclear Medicine (1986)

Haindl, Walter; Halpern, Samuel E.; Dillman, Robert O.; Hagan, Phillip L.; Beauregard, Jacquelyn; Clutter, Maureen L.; Amox, Diane G.; Ryan, Kevin; Bartholomew, Richard M.; Frincke, James M.; Carlo, Dennis J. Author Information
QUANTITATIVE ANALYSIS OF PERICARDIAL LATE GADOLINIUM ENHANCEMENT (LGE) WITH MYOCARDIUM AS THE REFERENCE REGION PERFORMS BETTER THAN VISUAL ASSESSMENT IN PATIENTS WITH CONSTRICTIVE PERICARDITIS
Authors: Paul Cremer, Muhammed Tariq, Abhishek Karwa, Scott Flamm, Allan Klein, Deborah Kwon
Journal: Journal of the American College of Cardiology (2015)
Abstract 1892: Integrated energetics and flux analysis reveals differential metabolic reprogramming in highly and less invasive cancer cells.
Authors: Abhinav Achreja, Lifeng Yang, Hongyun Zhao, Juan C. Marini, Deepak Nagrath
Journal: Cancer Research (2013)

Abstract Cancer cell metabolism was investigated using systems biology tools such as metabolic flux analysis (MFA) to reveal intracellular cancer cell metabolic flux distribution. To obtain a complete role of nutrient-induced deprivation in metabolic reprogramming of cancer cells, we need to use metabolic flux analysis to determine unknown intracellular fluxes. MFA provides a framework for the estimation of intracellular fluxes and energy balance analysis (EBA) ensures the thermodynamic feasibility of the computed optimal metabolic fluxes. MFA uses stoichiometric and mass balance constraints to compute the intracellular fluxes. Here, we have developed an integrated energy balance and flux balance analysis framework. Our framework allows estimation of intracellular fluxes and reveals specific adaptations and dysregulation of metabolic pathways in ovarian cancer cells as a result of the nutrient (glucose and glutamine) deprivation conditions. Glutamine and glucose are known to be important for cancer cell survival, growth and progression. Thus, there is a strong motivation to study metabolic alternations caused by glucose and glutamine deprivation and uncover links between various fuel sources for highly and less invasive cancer cells. Our findings indicate that NAD+/NADH ratio regulates glutamine's direct effect on lactate production irrespective of the bioenergetic substrate requirement for different kinds of cancer cells. Our findings, using quantification of metabolites pertaining to central carbon and nitrogen metabolism provided clues as to which pathways relevant to glycolysis, the pentose phosphate pathway, the TCA, urea cycles and lipid cycles, amino acid and nucleotide metabolisms may be altered by glucose and glutamine in cancer cells. This resulted in identification of dominant factors which control cancer metabolism. We believe that development of our oncosecretomics based metabolic strategy provides an unprecedented opportunity for the development of nutritional supplementation that can deregulate the adaptations and alterations induced by various nutrients in cancer metabolism. Citation Format: Abhinav Achreja, Lifeng Yang, Hongyun Zhao, Juan C. Marini, Deepak Nagrath. Integrated energetics and flux analysis reveals differential metabolic reprogramming in highly and less invasive cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1892. doi:10.1158/1538-7445.AM2013-1892 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.
COMPREHENSIVE ASSESSMENT OF TYPE 2 MYOCARDIAL INFARCTION IN AT-RISK PATIENTS: RESULTS FROM THE CATHETER SAMPLED BLOOD ARCHIVE IN CARDIOVASCULAR DISEASES (CASABLANCA) STUDY
Authors: Hanna Kim Gaggin, Yuyin Liu, Asya Lyass, Roland Van Kimmenade, Shweta Motiwala, Noreen Kelly, Aditi Mallick, Parul Gandhi, Nasrien Ibrahim, Mandy Simon, Arianna Belcher, Jamie Harisiades, Joseph Massaro, Ralph D’Agostino, James Januzzi
Journal: Journal of the American College of Cardiology (2016)
Abstract 5192: GEP100 binding to HER2 receptor promotes lung cancer invasion and metastasis
Authors: Menju Toshi, Shigeru Hashimoto, Ari Hashimoto, Hiroshi Date, Hisataka Sabe
Journal: Cancer Research (2010)

Abstract HER2 overexpression is well known to be a poor prognostic factor in some of epithelial tumors, including breast cancer and lung cancer. Previous reports have shown that GEP100-Arf6-AMAP1 pathway is pivotal for significant population of breast cancer invasion originated from EGFR signaling. Here we have examined the involvement of this pathway in HER2 signal-induced lung cancer invasion and / or metastasis. Co-immunoprecipitation for the reconstitutive expression of both HER2 and GEP100 or ARNO, the other ArfGEF for Arf6, showed that GEP100, but not ARNO, bound HER2 receptor through its pleckstrin homology (PH) domain. The stimulation of reconstitutively expressed cells with EGF did not increase its binding. On the other hand, the starvation for 24hours decreased. We found that H522 lung adenocarcinoma cells overexpressed HER2 receptor, but less EGFR, compared with the other thoracic cancer cell lines, and that GEP100 endogenously bound HER2, neither EGFR nor HER3 in this cells. In vitro invasion of H522 cells was little promoted with EGF stimulation used as chemoattractants. It suggested that the signal transduction through GEP100 was mainly originated from HER2 signaling in H522 cells, not EGFR. In vitro invasive activity in H522 cells was decreased by approximately 40-60% with three conditions as follows: the deprivation of HER2 signaling with the selective inhibitor, GEP100 knockdown by siRNA, or the interruption of this binding with the exogenous expression of PH domain of GEP100. And the knockdown of Arf6 and AMAP1, the downstream molecules of GEP100, similarly decreased in vitro invasion. Immunohistochemical analyses of GEP100 and HER2 expression for 112 cases of resected specimens of human primary lung adenocarcinoma were performed and all samples were scored with the intensity of tumor lesion. These analyses showed that the intensity scores of GEP100 and HER2 were both statistically higher in pN2 cases than in pN1 cases. Furthermore, in cases of very strong HER2 expression (score; 3+), positive expression of GEP100 tended to be correlated with the high frequency of lymphatic metastasis (100% vs 61.5%, p=0.08). GEP100 binding to HER2 receptor promotes lung cancer invasion and metastasis. Taking account of the importance of GEP100 in invasive mechanisms related with both EGFR and HER2 signaling, this GEP100 molecule appears to be a quite promising target to prevent cancer invasion and metastasis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5192.
AAAS Panel Discusses Cancer And Aging
Authors: T. Reynolds
Journal: JNCI Journal of the National Cancer Institute (1991)

Journal Article AAAS Panel Discusses Cancer And Aging Get access Tom Reynolds Tom Reynolds Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 83, Issue 7, 3 April 1991, Pages 471–473, https://doi.org/10.1093/jnci/83.7.471 Published: 03 April 1991
Single Organ Metastatic Disease, a New Prognostic Factor for Overall Survival (OS) in Stage IV Non-Small Cell Lung Cancer (NSCLC)
Authors: L. Hendriks, J.L. Derks, P.E. Postmus, R.A. Damhuis, R.M. Houben, E.G. Troost, M.M. Hochstenbag, E.F. Smit, A.-M.C. Dingemans
Journal: Annals of Oncology (2015)
Abstract 3743: 18F-Fluorothymidine (18F-FLT) as tumor response predictor for anti-epidermal growth factor receptor antibody in human lung cancer xenograft
Authors: Satoshi Takeuchi, Songji Zhao, Yuji Kuge, Yan Zhao, Ken-ichi Nishijima, Toshiyuki Hatano, Yasushi Shimizu, Ichiro Kinoshita, Nagara Tamaki, Hirotoshi Dosaka-Akita
Journal: Cancer Research (2010)

Abstract Background: Specific inhibition of epidermal growth factor receptor (EGFR) is one of the most promising treatment in the clinical setting. Cetuximab, a chimeric monoclonal antibody that binds to the extracellular domain of EGFR, has activity in colorectal cancer, non-small cell lung cancer, and so on. Because cetuximab is very expensive and has unique side effects, the development of appropriate evaluation for the therapeutic effects is necessary to use this drug effectively. Measurement of tumor proliferative activity by PET using 18F-Fluorothymidine (18F-FLT) may serve to assess early therapeutic effect. The purpose of this study is to determine whether 18F-FLT is useful to evaluate response to cetuximab treatment at a very early time-point in human lung cancer cell line xenograft. Methods: Human tumor xenograft model was established in male BALB/c athymic mice by human lung cancer cell line NCI-H1975. These mice were assigned to two groups (n=5, each group): control and cetuximab-treated groups. Cetuximab was intra-peritoneally given 1.0 mg/body on the first day. 3H-FLT was given intravenously on day 3 and radioactivity in the tumor and other tissue samples (%Injected Dose/g/kg) was measured using liquid scintillation one hour later. Immunohistochemical labeling index (LI, %positive cells) staining of nuclear antigen Ki-67 was also studied in tumor tissues as a tumor cell proliferation marker. Results: 3H-FLT uptake was higher in tumors compared with any other organs. The mean value of 3H-FLT uptake in tumor was significantly decreased to 55% of control value (0.38 ± 0.03 %ID/g/kg in control group vs. 0.21 ± 0.07 %ID/g/kg in cetuximab-treated group; p &amp;lt; 0.05). As compared with control, Ki-67 LI was significantly decreased in cetuximab-treated group (21.8 ± 6.7 %positive cells in control group vs. 2.2 ± 1.2 %positive cells in cetuximab-treated group; p &amp;lt; 0.001), while there was no significant changes in tumor volume and body weight between two groups. Conclusions: In this animal model, 3H-FLT uptake and Ki-67 expression were decreased in cetuximab-treated group just two days after the treatment start. These results indicate 18F-FLT-PET can be a useful predictor to evaluate response to molecular targeted medicine such as cetuximab at a very early time-point of treatment before significant change of tumor size. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3743.
Accidental Ingestion and Overdosage Involving Psychopharmacologic Drugs
Authors: Howard M. Cann, Henry L. Verhulst
Journal: New England Journal of Medicine (1960)

AS new drugs are developed, their accidental over-ingestion will occur, and, although toxicity studies on laboratory animals precede general distribution of drugs, human data usually become available only after distribution. Knowledge concerning the potential toxicity of psychopharmacologic agents is accumulating as they continue to be used. The information on the acute toxicity of these drugs in human beings presented below is based on 280 cases of accidental ingestion and poisoning involving "tranquilizer" agents reported to the National Clearinghouse for Poison Control Centers by 60 local poison control centers over a two-year period.Methods and Case Material StudiedIn the event . . .
AB0156 Expression of autophagy “markers” in monocytes from patients with active early rheumatoid arthritis:
Authors: C. Iannuccelli, R. Misasi, M. Di Franco, C. Alessandri, A. Capozzi, E. Lococo, M. Olivieri, M. Sorice, G. Valesini
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Autophagy is a fundamental self-degradative process in eukaryotic cells via the lysosomal pathway, involved both in basal turnover of cellular components and in the response to nutrient starvation (1). The immune system utilizes autophagy to restrict intracellular pathogens and regulate adaptive immunity, thus autophagy, as well as apoptosis, could be relevant in induction or loss of self tolerance to intracellular molecules and might act as a source of autoantigens in autoimmune diseases (2). <h3>Objectives</h3> Aim of our study was to study the monocyte autophagy in patients with active early RA naïve to steroids and DMARDs before and after 3 months of therapy. <h3>Methods</h3> 17 early RA patients (mean age 49 yrs, mean disease duration 21 weeks), fulfilling both 1987 and 2010 ACR criteria, naïve to treatment, were recruited after written informed consent. Before (T0) and after 3 months of treatment (T1), DAS28 was calculated, CRP was assessed. Autophagy markers (LC3, Ambra, beclin) were evaluated by flow cytometry on isolated monocytes. As a control group, 10 healthy donors were also evaluated. <h3>Results</h3> As expected, CRP, GH and DAS 28 score were significantly reduced following treatment with steroids and DMARDs (Table 1). Autophagy markers (LC3, Ambra, beclin) showed a similar behaviour, reducing their expression in monocytes after treatment (Tab.1). Interestingly, the expression of autophagy markers was significantly related to DAS28 (p=0.04 for LC3, p=0.03 for Ambra), to GH (p=0.01 for Ambra), and to CRP (p=0.01 for Ambra, p=0.006 for beclin). <h3>Conclusions</h3> These data indicate an increased expression of autophagy markers in isolated monocytes from patients with active early RA, suggesting a possible role for autophagy in the pathogenesis of this disease. The relationship between autophagy and acute inflammation is supported by the significant reduction of autophagy markers after therapy, together with their relationship with DAS28, GH, and CRP. Our results may contribute to clarify whether endogenous or pharmacological regulation of autophagy might modify the autoimmune response and the progression of the disease. <h3>References</h3> Lleo A et Al,J.Autoimmun 2007;29:61-68. Deretic V,Curr Opin Immunol 2009;21:53-62. <h3>Disclosure of Interest</h3> None Declared
AB0521 A single-arm, open-label study of the safety and effectiveness of tocilizumab in combination with methotrexate, in patients with rheumatoid arthritis in latin america – preliminary effectiveness results-ritmo study
Authors: C. Zerbini, J. Orozco, M. Martinez, J. Jaller, A. Quinteros, Y. Finol, J. Facal, R. Villacís, J. Alfaro, P. Faustino, R. Sáenz
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> To present the preliminary effectiveness data of TCZ + MTX treatment, with regard to reduction in signs and symptoms over 6 months in Latin American pts with mod/severe RA, MTX-IR. <h3>Methods</h3> Ritmo is a single-arm, open-label, prospective study, performed at 69 sites (18 Brazil, 12 Colombia, 9 Chile, 7 Mexico, 6 Argentina, 5 Ecuador, 4 Peru, 4 Costa Rica, 3 Venezuela, 1 Uruguay). Pts received TCZ 8 mg/kg IV/4wks + MTX(10-25mg/wk). Endpoints at wk24 included proportion of pts with ACR20/50/70 and DAS28 &lt;2.6, change in DAS28, EULAR resp. and HAQ. Clinical instructions (e.g.AES handling) were similar to the ones in Phase III trials. <h3>Results</h3> 362 patients were included (ITT). Mean age was 51 yrs, 88% women, with mean disease duration of 11yrs. RF positive in 79% of pts. Mean±SD Baseline DAS28 was 6.45±1.0 and mean±SD HAQ-DI score 1.55±0.7. 319 pts (88%) completed the 24wk treatment. At wk24, 63% of pts (95% CI: [57.9; 68,3]) had achieved the primary objective (ACR50). ACR20/70 rates were 72.6% (95% CI: [67.6; 77.2]) and 20.8% (95% CI: [16.7; 25.5]), respectively. DAS28 &lt;2.6 was achieved by 54.4% of pts. Mean±SD change in DAS28 score was 3.9±1.43 (p&lt;0.0001). Good and moderate EULAR responses were observed in 73.3% and 24.3% of pts, respectively. Mean±SD change in HAQ-DI was 0.92±0.7 (p&lt;0.0001). TCZ safety profile was comparable to that previously observed. Figure 1: ACR 20/50/70 responses over 24 weeks. <h3>Conclusions</h3> At wk24, significant improvement was observed and the safety profile of TCZ was comparable to that previously observed. These results suggest that TCZ is a therapeutic option in our region as suggested by data from the Pivotal Trials in DMARD-IR population.<sup>1-3</sup> <h3>References</h3> Smolen J, et al. Lancet 2008; 371:987-97. Genovese M, et al. Arthritis Rheum 2008; 58:2968-80. Kremer et al. Arthritis Rheum 2011;63(3):609-21. <h3>Disclosure of Interest</h3> C. Zerbini Grant/Research support from: Roche, Jansen, Pfizer, Sanofi-Aventis, J. Orozco Grant/Research support from: Roche, M. Martinez Grant/Research support from: Roche, J. Jaller Grant/Research support from: Roche, A. Quinteros Grant/Research support from: Roche, Y. Finol: None Declared, J. Facal: None Declared, R. Villacís: None Declared, J. Alfaro: None Declared, P. Faustino Employee of: Roche, R. Sáenz: None Declared