tbuckley/cabot-search huggingface dataset:
Abstract 407: Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line
Authors: I-Chen Wu, Yu-Kuei Chen, Deng-Chyang Wu, Ming-Tsang Wu
Journal: Cancer Research (2010)

Abstract Background: Once diagnosed, esophageal cancer has a very low overall 5-year survival rate. This study investigates the mechanisms behind the invasiveness and severity of esophageal squamous cell carcinoma (ESCC). Materials and Methods: We used Transwell Invasion Chamber to subdivide one Taiwanese esophageal squamous cancer cell line CE81T/VGH into sublines (CE81T-0, CE81T-1, CE81T-2, CE81T-3, and CE81T-4) in four rounds of assays, identified the most invasive ones, and measured various factors related to their invasiveness. Results: CE81T-1, CE81T-2, CE81T-3, and CE81T-4 sublines were significantly more invasive than the parental cell (CE81T/VGH) and CE81T-0 subline. CE81T-1 and CE81T-4, the sublines we chose to study further, had significantly greater colony forming ability (3.5-2.7 fold) and wound migrating activity (1.95-2.6 fold) than the parental cell in vitro (p&amp;lt; 0.01). They also displayed greater tumorigenesis in immunodeficiency BALB/c Foxlnn mice than the parental cell. We found an inverse correlation between expression of TIMP-2 (tissue inhibitor of metalloproteinase-2) and invasive ability and a significant positive correlation between expressions of MMP-1 (matrix metalloproteinase-1), vimentin, and p-Src (pY416) in these cell lines and their invasiveness (all p&amp;lt; 0.05). Conclusion: The subline model may be used to study the molecular and genetic mechanisms underlying the invasion and metastasis of ESCC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 407.
Abstract 3669: Bisphosphonate use following first primary ER+ breast cancer and risk of second primary breast cancer
Authors: Genevieve M. Monsees, Polly Newcomb, Kathi Malone, Christopher Li
Journal: Cancer Research (2011)

Abstract Background: Recent studies suggest that bisphosphonates, drugs commonly prescribed for the prevention and treatment of osteoporosis, may reduce risk of developing a first breast cancer. This is the first population-based assessment of the effect of bisphosphonate use on risk of developing a second breast cancer among breast cancer survivors. Methods: Within a population-based nested case-control study of women diagnosed with a first primary ER+ invasive breast cancer (BC) at ages 40-79, we assessed the association between bisphosphonate use after first breast cancer diagnosis and risk of asynchronous primary in the contralateral breast (CBC). Conditional logistic regression was used to compare 359 CBC cases to 649 incidence density matched controls (women not diagnosed with CBC). Analyses of duration were restricted to women followed for similar time periods. Results: Use of nitrogenous bisphosphonates for ≥12 months following first primary ER+ BC diagnosis was associated with a 56% decrease in CBC risk relative to no bisphosphonate use in this period (95% CI: 0.24-0.80, p-value: 0.008). Increasing duration of bisphosphonate use was associated with greater reductions in CBC risk (OR≥6months: 0.52, 95% CI: 0.31-0.89, p-value: 0.02; OR≥16months: 0.42, 95% CI: 0.21-0.83, p-value: 0.01; OR≥36months: 0.25, 95% CI: 0.07-0.84, p-value: 0.03). Use within 6 months also appeared protective (OR: 0.50, CI: 0.29-0.88, p-value: 0.02). Conclusions: This study suggests that use of nitrogenous bisphosphonates, following diagnosis with ER+ BC, may reduce second primary CBC. Given bisphosphonate tolerability and other potential benefits in this high-risk population, bisphosphonate therapy may be a feasible approach for CBC risk reduction, and merits further investigation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3669. doi:10.1158/1538-7445.AM2011-3669
BM-31 * STUDY OF INCIDENCE AND ESTIMATION OF RISK FACTORS ASSOCIATED WITH HIPPOCAMPAL METASTASIS
Authors: N. Sesikeran, Y. Sarma, K. Bhattacharya, V. Reddy, V. Reddy
Journal: Neuro-Oncology (2014)

BACKGROUND: Modern radiation techniques are being used to attempt hippocampal-sparing whole brain RT in brain metastasis in order to minimize neurocognitive decline. However this technique is accompanied by potential risk of hippocampal recurrence. AIMS: To evaluate the incidence of metastasis in the hippocampal region and identify factors which can predict the occurrence of metastasis in this region. METHOD: Pre-treatment MRIs of 40 cases(median age 55)with single or multiple brain metastases between Jan 2010-Dec 2013 were analysed. The hippocampus was contoured on 1mm thick planning-MRI on T1 image as per RTOG guidelines. All gross lesions were contoured, volume and minimum distance from hippocampus measured. Hippocampus was expanded by 5mm to represent peri-hippocampus. All lesions within this volume were considered as "hippocampal region metastases". Various clinical parameters were analysed by Pearson's test for correlation with presence of hippocampal disease. RESULTS: A total of 120 lesions were found in 40 patients.Incidence of metastasis in the hippocampal region was 20%(7.5% intra-hippocampal, 12.5% peri-hippocampal) of which 50% had breast primary, 27.5% lung,12.5% endometrium. Primary was controlled in 62.5%, 62.5% had received chemotherapy before diagnosis of brain metastasis. 87.5% of patients with hippocampal disease also had lung metastasis. Median aggregate volume and number of brain metastasis was 5.5cc and 2,while in those with hippocampal disease it was 23.81cc and 6. Among the parameters tested for correlation with presence of hippocampal metastasis, median aggregate volume(p = 0.001) and number of metastasis (p = 0.0001) had significant correlation, while presence of lung metastasis had a trend towards significance (p = 0.09). CONCLUSION: Metastasis in the hippocampal region are more likely to be present in patients who have larger number, larger aggregate volume of brain metastases and advanced systemic disease(lung-metastasis).
P655GRK2 subcellular localization in response to radiation stress in cardiac myocytes
Authors: A Franco, R Annunziata, R Pacelli, N Prevete, B Trimarco, D Sorriento, G Iaccarino, M Ciccarelli
Journal: Cardiovascular Research (2014)

GRK2 is an ubiquitous 80kd protein that interacts with several molecular partners in different cellular compartments. Recent studies have shown in endothelial cells that GRK2 localizes within the mitochondria, improves biogenesis and protects from ATP loss in response to ischemia/reperfusion. To evaluate cellular trafficking of GRK2 in isolated adult ventricular cardiomyocytes (AVM) from C57BL6 mice under stress condition, we isolated and cultured AVM, exposed them to 4Gy by Isovolt DS-1x-ray tube (Seifer), and subjected to differential centrifugation to isolate mitochondria, plasma membranes and cytosol fractions. GRK2 subcellular localization was evaluated by western blot at 3 and 8 hours from ionizing radiation exposure. At 3 hours from irradiation GRK2 is mainly localized in membranes, and its level are reduced in mitochondria. At 8 hours from irradiation, GRK2 accumulates into mitochondrial. Giving the role of GRK2 in the regulation of mitochondrial function we analyzed the production of reactive oxygen species (ROS) with DCHF (10-3M) and mitochondrial mass by Mito Traker (5nM) at 3 and 8 hours after irradiation. We found no modification in ROS production but reduction in mitochondrial mass after 3 hours from irradiation. After 8 hours, ROS production was increased along with mitochondrial mass. These date suggest that GRK2 subcellular localization is regulated in a time dependent manner in response to irradiation stress and it is associated with relevant modification of mitochondrial function. In conclusion, the regulation of GRK2 subcellular localization appears pivotal for the regulation of cardiomyocytes mitochondrial function in stress condition.
Model State Uses Cigarette Tax To Fund Cancer Research
Authors: L. Baute
Journal: JNCI Journal of the National Cancer Institute (1989)

Journal Article Model State Uses Cigarette Tax To Fund Cancer Research Get access Linda Baute Linda Baute Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 81, Issue 23, 6 December 1989, Pages 1772–1773, https://doi.org/10.1093/jnci/81.23.1772-a Published: 06 December 1989
OP0250 Distinct Imaging Patterns of Heart Failure in Systemic Lupus Erythematosus: Evaluation Using Cardiovascular Magnetic Resonance
Authors: S. Mavrogeni, G. Karabela, E. Stavropoulos, S. Plastiras, G. Spiliotis, E. Gialafos, G. Kolovou, P.P. Sfikakis, G.D. Kitas
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Systemic lupus erythematosus (SLE) is associated with increased cardiovascular disease either due to disease <i>per se</i> or to non-SLE related causes. We hypothesized that cardiac tissue characterization by cardiovascular magnetic resonance (CMR) may aid in clarifying the pathophysiology of heart failure in SLE. <h3>Methods</h3> Thirty-two SLE patients (29 women), aged 29±7 yrs (range 25-40), after recent X-Ray coronary angiography (&lt;30 days), due to new-onset HF NYHA I-II, were consecutively evaluated by CMR, including LV function, STIR T2 and late gadolinium enhanced (LGE) images. <h3>Results</h3> T2 ratio&gt;2 and patchy, epi/endo-myocardial LGEs with a total extent of 7±2% LV, due to acute myocarditis, were identified in 5/31 (16%) patients. LV function impairment, but without inflammation or fibrosis, due to dilated cardiomyopathy (DCM), was also found in 5 other patients (16%). A transmural myocardial scar (6 inferior and 5 anteroseptal), due to myocardial infarction, was evident in other 11/32 patients (34%), with an extent of 25±3% LV. Notably, diffuse subendocardial fibrosis with an extent of 34±3% LV, due to vasculitis, was revealed in 9 patients (29%). Finally, aortic valve stenosis and mitral valve regurgitation, due to Libman- Sacks endocarditis, were diagnosed in the remaining 2 patients (6%). No mixed pathology was observed in any patient. The presence of LGE, denoting myocardial fibrosis, correlated significantly with longer disease duration, higher erythrocyte sedimentation rate and the presence of anti-phospholipid antibodies Abnormal X-Ray coronary angiography (3, 5 and 3 patients having 1, 2 and 3 vessel disease, respectively) was found only in those patients with transmural myocardial scar, which followed the distribution of diseased vessels. <h3>Conclusions</h3> Heart failure in patients with SLE is due to various causes, including myocarditis, dilated cardiomyopathy, coronary artery disease, vasculitis and/or valvular disease. Assessment of the pathophysiologic background by CMR may facilitate the differential diagnosis and patients9 risk stratification. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3774
ORPHENADRINE IN THE TREATMENT OF DEPRESSION
Authors: JONAS B. ROBITSCHER, SYDNEY E. PULVER
Journal: American Journal of Psychiatry (1958)

In this preliminary study, 14 psychiatric patients with depressive features were treated with orphenadrine to determine if the euphoric action, previously reported as a side-reaction in nonpsychiatric patients, occurs and is beneficial in depression. Two of the patients were rated as recovered; 9 showed beneficial effects including improved behavior, more optimism concerning the future, decreased anxiety and agitation. Further dies with adequate controls and a double blind technique are warranted to determine if orphenadrine can prove useful in the treatment of depressed psychiatric patients.
AB0445 Vasculitides with necrotic lesions in different nosological entities in bulgarian population
Authors: D. Kalinova, T. Yoneva, V. Reshkova, R. Rashkov
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The vasculitides are a heterogenous group of diseases involving inflammation of blood vessels with subsequent tissue and/or organ failure.<sup>1</sup> Systemic vasculitis may be idiopathic or secondary associated with variety of diseases of known etiology, as one of the most severe clinical features are the necrotic changes of the extremitas and/or internal organs.<sup>2</sup> <h3>Objectives</h3> The objective of this study was to analyse a group of patients with different forms vasculitides, presenting with necrotic changes in relation to nosological belonging, association with other vasculitis lesions and immunological profile. <h3>Methods</h3> The study included 76 patients (19 men and 57 women) with vasculitic necrotic lesions, as the mean age was 59.7 years. The diagnoses were determined using the diagnostic criteria for systemic lupus erythematodes (SLE), progressive systemic sclerosis (PSS), rheumatoid artritis (RA), ANCA-associated vasculitis (AAV). Sera were tested for panel antinuclear antibodies (ANA) (anti-RibP, dsDNA, PCNA, Ro60, La, CENT-B, Sm, RNP/Sm, nucleosomes, Jo-1, Scl-70, Pm/Scl), for some antiphospholipid antibodies (anti-anticardiolipin, b2GPI, protrombin) and antineutrophil cytoplasmic antibodies (ANCA). <h3>Results</h3> The most frequent nosological entities associated with vasculitis, presenting with necrotic lesions, were SLE and PSS (44%). Hypersensitivity vasculitis with known and unknown etiology was diagnosed in 35.5%. AAV was determined only in 6.58% from the group with necrosis. It is interesting that rheumatoid artritis was associated with necrotic vasculitic changes in 7.9% of cases. Idiopathic vasculitis with necrosis wasn’t observed in the study. Analising other vasculitic lesions we found association with Raynaud’s phenomenon in 85.53%, cyanotic spots – 40.79%, livedo reticularis in 7.89% of patients, periungual vasculitis – 23.86%. The most frequently expressed antinuclear antibodies were anti-topoisomerase Abs (anti-Scl-70) – in 45% of patients. Other ANA were rarer detected. Antiphospholipid Abs were present in 33.3% of necrotizing vasculitis. ANCA were observed only in 3.75% of patients with necrosis. <h3>Conclusions</h3> Vasculitis with necrosis is developed in the course of a variety of diseases, as it is associated with other vasculitic lesions in the significant part of patients. It is supposed different antibodies participate in the pathogenetic mechanisms connecting with the damage of the endothelium, following endothelial dysfunction, which promotes vascular inflammation. <h3>References</h3> Merkel P et Mahr AD: Classification and epidemiology of vasculitis. Rheumatology, ELSEVIER, Toronto, Canada, 2011: 1501 Churg J. Systemic necrotizing vasculitis. Cardiovascular pathology, 1994, 3: 197-204. <h3>Disclosure of Interest</h3> None Declared
Paraganglioma Seen with FDG Dual-Head Gamma Camera Coincidence Imaging After False-Negative Results of I-123 MIBG Imaging
Authors: KAZUKI FUKUCHI, TAKASHI INENAGA, YOSHIHIKO SUZUKI, YOSHIO HORITA, KOHEI HAYASHIDA, YOSHIO ISHIDA
Journal: Clinical Nuclear Medicine (2001)

FUKUCHI, KAZUKI M.D.; INENAGA, TAKASHI M.D.; SUZUKI, YOSHIHIKO M.D.; HORITA, YOSHIO M.D.; HAYASHIDA, KOHEI M.D.; ISHIDA, YOSHIO M.D. Author Information
S3 T Cell Responses to Vitamin D Are Blunted in Latent Tuberculosis
Authors: G Hawthorne, J Zhang, L Jeffery, D Thickett, A Turner
Journal: Thorax (2012)

<h3>Introduction</h3> Low vitamin D status is associated with an increased risk of Mycobacterium Tuberculosis. Consistent with this, the active form of vitamin D, 1,25(OH)<sub>2</sub>D<sub>3</sub> exerts potent effects on immune cells. T cells are thought to contribute to the control of latent tuberculosis in which patients are infected with Mycobacterium tuberculosis but show no signs or symptoms of the disease. How vitamin D influences T cell responses in latent patients is therefore of interest. It has been shown that the co-suppressive protein, CTLA4, which is expressed on regulatory T cells and induced in T cells upon activation, is strongly up regulated by vitamin D and that it increases the frequency of CTLA-4+FoxP3+ cells [1]. Furthermore, vitamin D conditioned T cells are functionally suppressive. In this study we have therefore compared the effect of vitamin D upon CTLA-4 expression and the frequency of CD25+ FoxP3+ CTLA4+ cells in healthy and latent TB patients. <h3>Methods</h3> Peripheral blood cells from healthy control donors (n=21) and patients with latent tuberculosis (n=12) were cultured with SEB or PPD with or without 1,25(OH)2D3 or inactive 25(OH)D3. CD25+ FoxP3+ CTLA4+ frequency and the median CTLA-4 expression (MFI) of CD25+ cells was then monitored after two days by flow cytometry. <h3>Results</h3> 1,25(OH)<sub>2</sub>D<sub>3</sub> significantly increased CTLA4 MFI in both healthy and latent populations following stimulation with SEB (p≤0.01) or PPD (P=0.026, 0008). 25(OH)D<sub>3</sub> also enhanced CTLA-4 expression in SEB cultures (p≤0.01). Induction of CTLA-4 was however reduced in PPD cultures (median 121) compared to SEB (median 360). Interestingly, the magnitude of CTLA-4 induction by 1,25(OH)<sub>2</sub>D<sub>3</sub> or 25(OH)D<sub>3</sub> also differed for healthy and latent populations in response to SEB (1,25(OH)<sub>2</sub>D<sub>3</sub> (p=0.01) and 25(OH)D<sub>3</sub> (p=0.006), with a similar trend in PPD stimulated cells (p=0. 092). <h3>Conclusion</h3> The shift towards a T reg population as a result of vitamin D is blunted in latent TB compared to health. Differential response of memory cells in latent disease could account for this. Jeffery, LE, et al., <i>1,25-Dihydroxyvitamin D3 and IL-2 combine to inhibit T cell production of inflammatory cytokines and promote development of regulatory T cells expressing CTLA-4 and FoxP3.</i> Journal of immunology, 2009. <b>183</b>(9): p. 5458–67.
Pneumatosis Cystoides Intestinalis of the right colon: EUS findings, interesting cause and feasibility of interesting treatment
Authors: T Hirano
Journal: Endoscopy (2006)

Pneumatosis Cystoides Intestinalis (PCI) of the colon is a relatively rare condition. We report two cases of PCI of the right colon and EUS findings. In the first case, PCI was caused by Voglibose (alpha 1-glucosidase inhibitor) for treatment of diabetes mellitus, which is the fifth case in the Japanese literature and no case reports from outside Japan. In the second case, a submucosal tumor of PCI was shrunken by needle aspiration. This aspiration method may be feasible for acute relief of symptoms in colonic obstruction due to PCI.
AB0238 Treatment of rheumatoid arthritis patients with chronic hepatitis b: analysis of korean national health insurance claims data
Authors: K. Shin, E. B. Lee, E. Y. Lee, Y. W. Song
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> There are currently limited treatment options for rheumatoid arthritis (RA) patients with chronic hepatitis B (CHB). Numerous recommendations or consensus have been published for selecting conventional and biologic disease modifying anti-rheumatic agents (DMARDs) for these patients. However, there is limited data demonstrating what DMARDs are practically prescribed within the population of an endemic region of CHB. <h3>Objectives</h3> To study the prescription characteristics of conventional and biologic DMARDs, as well as the rate of combination of anti-viral agents in RA patients with CHB in Korea. <h3>Methods</h3> We analyzed the Korean National Health Insurance claims database (2007-2009) of RA patients through the co-work with Korean Health Insurance Review and Assessment Service. Patients with CHB were defined by an algorithm including prescription information, blood tests, and the ICD-10 code. Among biologic DMARDs, data of etanercept, adalimumab, infliximab, and rituximab data were only available during the study period. <h3>Results</h3> There were 8,677 CHB patients (3.8%) among 226,592 RA patients in the database. The age distribution or gender difference in CHB patients was comparable to the general RA population. Hydroxychloroquine was the most frequently (66.2%) prescribed DMARD. Thirty four percent of CHB patients had been prescribed with methotrexate (MTX) during the study period, only 7.6% with concomitant anti-viral treatment. About 3% of RA patients with CHB were prescribed with TNF inhibitors, which was significantly lower compared with the rate in the general RA population in Korea. <h3>Conclusions</h3> MTX is yet one of the DMARDs frequently prescribed to Korean RA patients with CHB. Our data also indicate that biologic DMARDs are indeed seldom prescribed in this particular subgroup of patients. <h3>Disclosure of Interest</h3> None Declared
AB0931 Anti-TNF Agents in Intractable Familial Mediterranean Fever with Axial Spondylarthropathy: Four Cases
Authors: M. Rozenbaum, L. Kaly, D. Rimar, G. Slobodin, N. Boulman, N. Jiries, I. Rosner
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Familial Mediterranean Fever (FMF) is an autoinflammatory disease characterized by recurrent attacks of fever and serositis. A relation between FMF and Ankylosing Spondylitis (AS) has been suggested in small cohort studies, although there is no consensus regarding the role of HLA B27. Colchicine, the mainstay treatment in FMF, does not improve the axial or peripheral symptoms due to spondylarthropathy. There are controversial data about the efficacy of Tumor Necrosis Factor Alpha (TNF α) blockade in FMF patients (1). <h3>Objectives</h3> Efficacity of Tumor Necrosis Factor Alpha in FMF patients with axial spondyloarthropathy. <h3>Methods</h3> We report our experience in 4 patients with intractable FMF treated with oral colchicine and supplemental IV colchicine (2), that were treated with TNF α blockade for symptomatic axial spondylarthropathy). <h3>Results</h3> A 26- year-a old man with MEFV mutations V726A and E148Q, negative for HLAB27, with concomitant ulcerative colitis was treated with infliximab and then with adalimumab; and 3 women (42, 48 and 55 years old), two of them treated with infliximab and one treated with adalimumab. The three women were homozygous for the M694V mutation. All developed severe to moderate adverse events: exacerbation of FMF in 2 of them, and myositis and ulcerative colitis exacerbation in the male patient, and staphylococcus aureus sepsis in another patient. Three of them had to stop the TNF α blockade treatment. One patient developed psoriatic rash, with no need to stop the treatment. <h3>Conclusions</h3> In our limited experience, TNF α blockade in patients with both intractable FMF and AS is not very effective and may be associated with severe adverse events. Little is known about the possible interaction between intravenous colchicine and anti-TNF treatment. <h3>References</h3> Bilgen SA, Kilic L, Akdogan A, Kiraz S, Kalyoncu U, Karadag O, Ertenli I, Dogan I, Calguneri M. Effects of anti-tumor necrosis factors agents for familial Mediterranean fever patients with chronic arthritis and/or sacroiliitis who were resistant to colchicine treatment. J clin Rheumatol 2011;7:358-62. Rozenbaum M, Boulman N, Feld J, Avshovich N, Petrovich S, Elias M, Slobodin G, Rosner I. Intravenous colchicine treatment for six months: adjunctive therapy in Familial Mediterranean fever (FMF) unresponsive to oral colchicine. Clin Exp Rheumatol 2009; 27 (2 suppl 53) S 105. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.1994
P1117 SIMEPREVIR REDUCES TIME WITH PEGINTERFERON/RIBAVIRIN-INDUCED SYMPTOMS AND QUALITY-OF-LIFE IMPAIRMENTS: 72-WEEK RESULTS FROM THREE PHASE III STUDIES
Authors: J. Scott, L. Gilles, M. Peeters, K. Cerri, M. Beumont-Mauviel
Journal: Journal of Hepatology (2014)