tbuckley/cabot-search huggingface dataset:
THU0018 Sumo specific proteases 5 and 7 contribute to the inbalanced sumoylation level of rheumatoid athritis synovial fibroblasts
Authors: S. Strietholt, M.A. Peters, S. Frank, A. Hillmann, G. Kollias, T. Pap
Journal: Annals of the Rheumatic Diseases (2012)

<h3>Background</h3> It has been shown previousely that posttranslational protein modification through SUMOylation is enhanced in rheumatoid athritis synovial fibroblasts (RASF) compared to osteoathritis synovial fibroblasts (OASF) and that higher levels of SUMOylation result in a reduced capability of RASF to undergo FAS induced apoptosis. <h3>Objectives</h3> Based on these findings, we here focussed on the expression of the SUMO specific proteases SENP5 and SENP7, both of which are essential for the maturation and deconjugation of SUMO2/3 from its target proteins. <h3>Methods</h3> Synovial tissue samples were obtained from RA and OA patients and used for cell culture experiments and histological analysis. The expression of SENP5 and 7 was analysed by PCR and Western Blot. With specific antibodies, the expression of SENP5 and 7 was analyzed by immunohistochemistry in synovial tissue specimens. The subcellular localization of SENP5 and 7 was studied by immunocytochemical costaining with cellcompartment specific markers and confocal laser scanning microscopy. <h3>Results</h3> We where able to show an imbalance in both SUMO specific protease 5 and 7. Both were strongly upregulated in RASF in comparison to OASF. SENP5 showed a clear nuclear localization whereas SENP7 was found also in the cytoplasm. In line with our findings in tissue samples we could show a expression of SENP5 and 7 in RASF compared to OASF. Furthermore we where able to confirm these findings in the hTNFtg mouse model of human RA. Here, we found a strong upregulation of SENP5 and 7 on protein level. However the mRNA level showed only a difference for SENP7. In immuncytochemistry experiments the nuclear and cytoplasmic staining for Senp7 could be proven. <h3>Conclusions</h3> Our findings underline the role of posttranslational protein modifications in RASF. Strong unbalances in the SUMOylations in hand with a striking overexpression of SENP's adds to the characteristic features of RASF that promote the diseases processes in RA fibroblasts. <h3>Disclosure of Interest</h3> None Declared
Sex differences in weight in infancy and the British 1990 national growth standards
Authors: C. M Wright, S. S Corbett, R. F Drewett
Journal: BMJ (1996)

<h3>Abstract</h3> <b>Objectives</b>: To determine if there is a sex difference in infancy in the new British national standards for weight (based on data from 1990). <b>Design</b>: Weight data in a birth cohort were compared with the 1990 standards and Tanner and Whitehouse (1966) standards up to age 12 months. <b>Setting</b>: Newcastle upon Tyne. <b>Subjects</b>: 3418 term infants. <b>Results</b>: Our cohort showed a mean difference in standard deviation scores of 0.42 between boys and girls (P&lt;0.0001) when compared with the 1990 standards. Two and a half times as many girls as boys had weights below the 3rd centile during the first year, with an equivalent excess of boys above the 97th centile (P&lt;0.0001). Similar results were found with Tanner and Whitehouse standards. <b>Conclusions</b>: These differences could result in substantial sex bias in the identification of poor growth in early childhood. The standards need modification.
THU0154 Disease Characteristics and Predictors of Development of RA: A 2-Year Follow-Up Study of 658 Patients with Undifferentiated Arthritis of Less Than 16 Weeks Duration
Authors: G. H. Brinkmann, E. S. Norli, E. Lie, A. J. Haugen, L. Grøvle, H. Nygaard, O. Bjørneboe, T. K. Kvien, M. D. Mjaavatten
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Identifying patients with undifferentiated arthritis (UA) who are destined to develop rheumatoid arthritis (RA) is important to allow early institution of disease modifying therapy. <h3>Objectives</h3> To describe and compare the baseline characteristics of very early UA-patients developing RA according to the 2010 ACR/EULAR RA classification criteria (UA-RA) vs. not developing RA (UA-non-RA) over a 2-year follow-up period. <h3>Methods</h3> An early arthritis clinic was established as a prospective multi-center cohort study in 2004. Patients (18-75 years old) with ≥1 swollen joint of ≤16 weeks duration were included and followed for 2 years with examination at 0, 3, 6,12 and 24 months. A comprehensive data collection was undertaken, including a structured patient history, 66-swollen (SJC) and 28-tender joint counts (TJC), laboratory examinations and patients reported outcomes. Patients with osteoarthritis, septic arthritis or arthritis due to trauma were excluded. Mann-Whitney U-test, independent T-test and chi-square tests were used as appropriate to compare baseline characteristics between UA-non-RA and UA-RA patients. <h3>Results</h3> 1085 patients (pts) were included from 2004 to 2010 (mean(SD) age 46.1(14.9) years, 54.7% females, median (interquartile range (IQR)) duration of joint swelling 33(12,65) days). 658 pts (after excluding pts with other diagnoses) had available data for application of the 2010 ACR/EULAR RA classification criteria at baseline, and 236/658 (35.9%) fulfilled the criteria. Of the remaining 422 UA pts, only 27 further pts (6.4%) were classified as RA according to the criteria during follow-up. Distribution of criteria points at baseline in UA-RA pts:5 points n=11, 4 points n=7, 3 points n=6, 2 points, 1 point and 0 points n=1 each. In contrast, 20/395 (5.1%), of the UA-non-RA pts had 5 criteria points at baseline (p&lt;0.001). 13/20 (65.0%) of these pts were seronegative compared to 4/11 (36.4%) of the UA-RA pts (p=0.13). 21/27 (77.8%) of the UA-RA pts fulfilled the criteria within the first 6 months. Comparison of baseline characteristics between the two groups is shown in the table. <h3>Conclusions</h3> Only 6.4 % of patients with very early UA fulfilled the 2010 RA classification criteria during 2 years of follow-up, most of them fulfilled the criteria within the first 6 months. In univariate analyses female gender, smoking, seropositivity, higher joint counts and disability were associated with RA development. The number of patients with 5 criteria points at baseline was higher in the UA-RA vs. the UA-non-RA group. <h3>Disclosure of Interest</h3> None Declared
Abstract 1972: Clinical impact of 6q deletion patterns in prostate cancer.
Authors: Martina Kluth, Nina Amschler, Anna Heinl, Simon Jung, Ronald Simon, Thorsten Schlomm, Guido Sauter, Sarah Jane Pauline Minner
Journal: Cancer Research (2013)

Abstract Deletions of chromosome 6q12-22 represent the second common deletion in prostate cancer. Although most of these deletions are large and span &amp;gt; 50 megabases, a minimal deleted region containing 3-4 megabases was described at 6q15. MAP3K7 (at 6q15) was suggested as a potential target gene in this area. Based on the hypothesis, that large 6q deletions compromising a multitude of genes may have a different impact on the biology of prostate cancer cells than small circumscribed 6q15 deletions, a tissue microarray (TMA) containing over 7000 prostate cancers was analyzed by fluorescence in situ hybridization (FISH). All cancers were analyzed with probes for 6q12, 6q14, 6q15 (MAP3K7), 6q16, and 6q22. 3888 tumors were interpretable for all 5 probes including 463 (10.6%) showing a 6q deletion. 6q deletions were tightly linked to a negative ERG status with 15.6% deletions in ERG negative and 5.8% deletions in ERG positive cancers (p&amp;lt;0.0001). The frequency of 6q deletions increased with Gleason grade and pT stage. 6q deletions were found in 5.4% of ≤3+3, 10.6% of 3+4, 19.6% of 4+3, and 18.8% of ≥4+4 cancers (p&amp;lt;0.0001). 6q deletions occurred in 9.6% of pT2, 11.5% of pT3a, and 14.8% of pT3b carcinomas (p=0.0160). 6q deletions were also linked to early PSA recurrence (p&amp;lt;0.0001). Remarkably, the clinical outcome varied markedly with deletion size. PSA recurrence was more likely in cancers with 6q12-22 deletions as compared to tumors with 6q14-16 deletions (p&amp;lt;0.0001). Twelve genes located within 6q14-16 were functionally evaluated applying colony formation assays to prostate cell lines BPH1, DU-145, and PC-3. These analyses failed to identify a cell behavior consistent with a role as a tumor suppressor gene for MAP3K7, as well as RRAGD, ANKRD6, LYRM2, MDN1, CASP8AP2, BACH2, COQ3, MANEA, RNGTT, SLC35A1, and MMS22L. In conclusion, the strong impact of the deletion size suggests, that multiple genes on chromosome 6q12-22 might have a tumor suppressive role in prostate cancer and that alterations of more than one of these has an additive adverse effect to cells. Our data do not confirm MAP3K7 as the main target of 6q deletions in prostate cancer Citation Format: Martina Kluth, Nina Amschler, Anna Heinl, Simon Jung, Ronald Simon, Thorsten Schlomm, Guido Sauter, Sarah Jane Pauline Minner. Clinical impact of 6q deletion patterns in prostate cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1972. doi:10.1158/1538-7445.AM2013-1972
THE VALUE OF ELECTROCARDIOGRAPHY FOR THE PROGNOSTICATION OF ACUTE PULMONARY EMBOLISM: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Amro Qaddoura, Genevieve Digby, Conrad Kabali, Piotr Kukla, Zhong-Qun Zhan, Adrian Baranchuk
Journal: Journal of the American College of Cardiology (2016)
An Evaluation of Tetracycline Preparations
Authors: William P. Boger, John J. Gavin
Journal: New England Journal of Medicine (1959)

SHARP editorial comments1 2 3 continue to appear4 regarding the claims and counterclaims that have been made for various tetracycline preparations. Specific criticisms have been made of much of the published literature.5 and certainly some of the errors of omission must be granted. However, if there is merit in pinpointing the chief reason for confusion in this "battle of blood levels," it can be done very readily — in retrospect! It has been time-honored practice to employ dicalcium phosphate as an "inert" excipient or filler in tablets and capsules. Although it is now fairly general knowledge that tetracycline acts as a chelating . . .
THU0456 Relapsing Polychondritis : Cases Series in China and Review of the Literature
Authors: D. Lin, J. Gu
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> To study the the clinical features of Relapsing polychondritis (RP) in China. <h3>Methods</h3> 158 cases from 1994-2012 reported in China were retrospected and compared with international literal reviews[1-8] including clinical features, systemic involvement, differential diagnosis and prognosis. <h3>Results</h3> 1)Average age of onset was 45.3 years old, the mean age at first symptoms was 14.4 months and sex ratio was 63 female to 95 male, misdiagnostic rate was as high as 47%. 2) contrast with literal reviews, arthritis was lower frequent than Caucians (18% : 23-47%), but higher than Japaese (5%), while laryngotracheal symptom was higher (42%,14-38%) at the time of diagnosis. The incidence of auricular chondritis(68%: 84% -95%), ocular inflammation (44% :49-65%) were lower during the course of follow-up. Renal (3%: 9% -26%)involvement was lower, and neurological involvement (13%: &lt;10%) were found to be higher in Chinese population. The proportion of associated autoimmune disease and systemic vasculitis were respectively 6%, 3% similar to Japaese (4%, 2%), but less than Caucasians(12-26%,5-18%). The top death cause is respiratory failure due to the primary disease, followed by lung infections and cardiovascular events. Teenager patients(&lt;18yrs) had higher incidence in arthiritis(84%:56%), nasel chondritis(84%:56%), largynochontreal involvement(79%:68%) compared to adult patients in China. <h3>Conclusions</h3> Chinese RP had its unique characteristics in spite of sharing most clinical features with the cases series in literature reviews. <h3>References</h3> McAdamLP, O'HanlanMA, BluestoneR, et al. Relapsingpolychondritis:prospective study of 23 patients and a review of the literature. Medicine1976;55(3):193–215. Suzuki M, Uchida K, Nagano M, et al. Case of relapsing polychondritis associated with persistent rib cartilage pain and severe tracheal stenosis--a review of 53 cases in Japan. Nihon Kyobu Shikkan Gakkai Zasshi. 1983 Jul;21(7):665-71. Japanese. Michet CJ Jr, McKenna CH, Luthra HS, et al. Relapsing polychondritis. Survival and predictive role of early disease manifestations. Ann Intern Med, 1986,104:74-78 Chang-Miller A, Okamura M, Torres VE, et al. Renal involvement in relapsing polychondritis. Medicine (Baltimore). 1987 May;66(3):202-17. Francès C, el Rassi R, Laporte JL, et al. Dermatologic manifestations of relapsing polychondritis. A study of 200 cases at a single center. Medicine (Baltimore). 2001 May;80(3):173-9 Zeuner M, Straub RH, Rauh G, Albert ED, Scholmerich J, Lang B. Relapsingpolychondritis: clinical and immunogenetic analysis of 62 patients. JRheumatol 1997;24(1):96–101. David E.Trentham, Christine H. Le, Relapsing Polychondritis. Ann Intern Med.1998;129:114-122. David E.Trentham, Christine H. Le, Relapsing Polychondritis. Ann Intern Med.1998;129:114-122. Alexandre Belot, Agnes Duquesne, Chantal Job-Deslandre, et al. Pediatric-Onset Repalsing Polychondritis: Case Series and Systematic Review. <h3>Disclosure of Interest</h3> None Declared
Association between Plasma Homocysteine Concentrations and Extracranial Carotid-Artery Stenosis
Authors: Jacob Selhub, Paul F. Jacques, Andrew G. Bostom, Ralph B. D'Agostino, Peter W.F. Wilson, Albert J. Belanger, Daniel H. O'Leary, Philip A. Wolf, Ernst J. Schaefer, Irwin H. Rosenberg
Journal: New England Journal of Medicine (1995)

Epidemiologic studies have identified hyperhomocysteinemia as a possible risk factor for atherosclerosis. We determined the risk of carotid-artery atherosclerosis in relation to both plasma homocysteine concentrations and nutritional determinants of hyperhomocysteinemia.
TEMPORAL TRENDS AND OPERATOR VARIATION IN ANTICOAGULANT USE DURING PERCUTANEOUS CORONARY INTERVENTION FOR PATIENTS WITH ACUTE MYOCARDIAL INFARCTION IN THE UNITED STATES
Authors: Eric Alexander Secemsky, Ajay Kirtane, Sripal Bangalore, Enrico Ferro, Neil Wimmer, Matthew Roe, Dadi (David) Dai, Laura Mauri, Robert Yeh
Journal: Journal of the American College of Cardiology (2016)
Vitamin E Deficiency with Normal Serum Vitamin E Concentrations in Children with Chronic Cholestasis
Authors: Ronald J. Sokol, James E. Heubi, Susan T. Iannaccone, Kevin E. Bove, William F. Balistreri
Journal: New England Journal of Medicine (1984)

We studied serum vitamin E levels and the ratio of serum vitamin E to serum lipid levels in 11 children with chronic cholestasis complicated by vitamin E deficiency, as defined by characteristic neurologic signs or sural-nerve histopathology in addition to impaired intestinal absorption of vitamin E. Eight of the children had low levels of serum vitamin E, as well as low ratios of serum vitamin E to total lipids and to cholesterol. However, three patients had normal serum vitamin E levels but low ratios of serum vitamin E to total lipids (two of the three had normal ratios of vitamin E to cholesterol). In four patients who were not vitamin E-deficient, all three values were normal. We conclude that vitamin E deficiency may exist in a child with a normal serum vitamin E concentration and that the ratio of serum vitamin E to total serum lipids is the most reliable biochemical index of vitamin E status during chronic childhood cholestasis.
Absence of Epstein-Barr Viral DNA in American Burkitt's Lymphoma
Authors: Joseph S. Pagano, Chien Hui Huang, Paul Levine
Journal: New England Journal of Medicine (1973)

The cellular DNA from tumor-infiltrated tissue from four patients with the American form of Burkitt's lymphoma did not contain detectable Epstein-Barr viral DNA. The analyses were conducted by a highly specific and sensitive molecular hybridization technic, DNA-DNA renaturation kinetics, which can detect 0.4 genome or less of Epstein-Barr viral DNA per cell. The absence of nucleotide sequences homologous to the virus in the American lymphomas is in contrast to African Burkitt's lymphoma and also to nasopharyngeal carcinoma, in which Epstein-Barr viral DNA is usually detectable even with the less sensitive technic of complementary RNA-DNA hybridization. Six specimens from patients with American Hodgkin's disease and three from patients with metastatic melanoma also did not contain detectable Epstein-Barr viral DNA. (N Engl J Med 289:1395–1399, 1973)
Abstract 3088: Regulation of tight junction protein claudin 1 mRNA expression by AU-rich element-binding (ELAV) proteins in colon cancer
Authors: Ashok Sharma, Amar B. Singh, Punita Dhawan
Journal: Cancer Research (2011)

Abstract Tight junction associated proteins are key molecular components governing cellular adhesion, polarity and play critical roles in cellular proliferation via their functions as couplers of the extracellular milieu to intracellular signaling pathways. Claudin-1, a tight junction protein, expression is dysregulated in the majority of human colorectal carcinomas. We have previously reported that claudin-1 is regulated at post-transcriptional levels on treatment with HDAC inhibitor, TSA and its mRNA stability is modulated by 3’-UTR as the major mechanism underlying HDAC-dependent claudin-1 expression. It has putative AU-rich (ARE) cis-elements in its long 3’UTR (∼ 2.3Kb). Presence of a long 3’UTR is frequently indicative of post-translational regulation of gene expression via modulation of mRNA stability. ELAV like RNA-binding proteins Hu antigen R (HuR) and tristetraprolin (TTP) associate with labile mRNAs bearing AU-rich elements located in the 3’ untranslated regions (3′-UTRs) and modulate their stability. We have identified putative AU rich elements in the proximal region of the claudin 1 3’-UTR and demonstrate that HuR interacts with these AU-rich elements. We further show a role of HuR and TTP during colon tumorigenesis and their ability to regulate Claudin-1 mRNA expression in presence TSA. We found that Claudin-1 mRNA was stabilized by HUR in both SW480 and SW620 cells, whereas its overexpression decreased the expression of TTP; We used a ribonomic approach to test the binding of HuR and TTP to claudin 1 3’UTR and their role in stabilization/destabilization of claudin-1 mRNA in the presence of TSA. On treatment with TSA the binding of HuR to claudin-1 3’UTR decreases while the binding of TTP increases correlating well with the rapid decay of claudin-1 mRNA in presence of TSA. Thus, our data suggest that claudin 1 is regulated via mRNA stability and a novel regulatory feedback loop between HuR and TTP in colon tumorigenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3088. doi:10.1158/1538-7445.AM2011-3088
CONTRAST MEDIUM INDUCED PD/PA RATIO (CMR) VERSUS FFR AND ADENOSINE-FREE INDEXES IN THE EVALUATION OF INTERMEDIATE CORONARY STENOSIS
Authors: Antonio Maria Leone, Pio Cialdella, Giovanni Luigi De Maria, Eloisa Basile, Giuseppe Zimbardo, Lazzaro Paraggio, Domenico D'Amario, Carlo Trani, Francesco Burzotta, Antonio Rebuzzi, Filippo Crea
Journal: Journal of the American College of Cardiology (2016)
Compound Reverses Memory Deficits in Model of Schizophrenia
Authors: Tracy Hampton
Journal: JAMA (2016)

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Pathogenesis of Malignant Histiocytic Lymphoma Induced by Silica in a Colony of Specific-Pathogen-Free Wistar Rats
Authors: Margaret M. F. Wagner
Journal: JNCI Journal of the National Cancer Institute (1976)

The occurrence of malignant histiocytic lymphomas (MHLT) after an intrapleural inoculation of silica and the presence of a hyperplastic or granulomatous reaction to the silica were confirmed in cesarean-derived barrier-maintained inbred W rats. A specific hyperplastic reaction in the silica granulomas preceded the appearance of MHLT. Hyperplastic changes were observed when the absolute monocyte count at weeks 25-30 was slightly higher than that seen in the controls. Silica injected intrapleurally penetrated the thymus tissue but the carbon and coal dusts and the silica, which was administered by other routes, did not involve the thymus. Also, silica was the sole dust, when introduced into the coelomic cavities that produced MHLT, which grew rapidly on entering the thymus. Rats that died with MHLT had widespread dissemination with malignant foci situated most consistently above the diaphragm.