tbuckley/cabot-search huggingface dataset:
Analysis of biomarker expression (ki67, EGFR, HER2, ERK, NFkB, and mTOR) in patients with advanced gastric and gastroesophageal junction (GEJ) cancer treated with cetuximab plus cisplatin/docetaxel: DOCETUX study.
Authors: C. Pinto, V. Mutri, F. Di Fabio, S. Giaquinta, C. Ceccarelli, F. L. Rojas Llimpe, P. Di Tullio, C. Barone, S. Siena, A. Martoni
Journal: Journal of Clinical Oncology (2010)

4133 Background: The aim of this study was to evaluate the correlation between pathologic biomarkers and objective response (OR), time-to-progression (TTP) and overall survival (OS) in advanced gastric or GEJ cancer patients (pts) treated with cetuximab plus chemotherapy. Methods: We analysed 31/72 pts with gastric or GEJ locally advanced/metastatic adenocarcinoma enrolled in the DOCETUX Study (Pinto et al., Br. J. Cancer, 2009). The pts were treated as first-line with cetuximab weekly plus cispatin/docetaxel every 3 weeks, for a maximum of 6 cycles, then cetuximab alone in pts with CR/PR/SD. In these pts KRAS/BRAF mutations were infrequent and not associated with efficacy (Stella et al., ASCO 2009, Abs 15503). Analyses of ki67, EGFR, HER2, ERK, NFkB and mTOR were performed using immunohistochemistry on primary tumor by just one pathologist. Results: Pt characteristics: 27M/4F; median age 62 years (24-74); stomach 28 (90.3%), GEJ 3 (9.7%); intestinal histotype 24 (77.4%), nonintestinal 7 (22.6%); locally advanced disease 1(3.2%), metastatic disease 30(96.8.%). OR were: 15 (48.4%) CR+PR, 10 (32.3%) SD, 4 (12.9%) PD, 2 (6.5%) NE. Median TTP was 5 months (1-23), and median OS 11 months (1-26). The expression of pathological biomarkers was: ki67 > 40% in 83.9% of pts, EGFR score high in 26.1%, HER2 2+-3+ in 28.6%, ERK > 30% in 48.4%, NFkB > 5% in 43.5% and mTOR > 2% in 58.1%. In the multivariate analysis, the better OR was observed in pts with negative/low EGFR (p = 0.017) and high ERK (p = 0.035), and a longer TTP in pts with positive mTOR (p = 0.036). Any other statistically significant correlation was observed. Conclusions: This results suggest that negative/low EGFR score, high ERK and positive mTOR expressions are correlated to treatment efficacy. No significant financial relationships to disclose.
Oncogenicity of Plaque Variants of CELO Virus in Newborn Hamsters 2
Authors: R. G. Pendola, V. J. Yates, R. E. Wolke, V. Jasty
Journal: JNCI Journal of the National Cancer Institute (1973)

Small-plaque (SP) and large-plaque (LP) variants of chicken-embryo-lethal-orphan (CELO) virus, an avian adenovirus, were tumorigenic in newborn hamsters inoculated either subcutaneously (sc) or intraperitoneally (ip). Tumors were induced at the site of sc inoculation. With the ip route, tumors were induced in the peritoneal cavity and liver. No significant difference was observed either in the latent period of induction or in tumor incidence between the two virus variants. The neoplasms induced in subcutaneous areas and in the peritoneal cavity were classified histologically as anaplastic sarcomas; the liver tumors were bile-duct carcinomas. The sera From the SP and LP tumor-bearing hamsters contained CELO-specific T antibodies.
4P Studies of structure-functional peculiarities of soluble Her2 secreted by breast tumor cells
Authors: Y.A. Trizna, K.A. Glukhova, A.S. Glukhov, V.V. Marchenkov, B.S. Melnik, G.V. Afanasieva, A.A. Ivanov, O.P. Popova, T.I. Danilova, I.P. Beletsky, O.V. Proussakova
Journal: Annals of Oncology (2015)
Abstract 2903: Differential regulation of LC3 A and B, GABARAPL 1 and 2 autophagy genes by micro-environmental stress and role in breast cancer survival
Authors: Christos E. Zois, Syed Haider, Simon Wigfield, Alexandra Giatromanolaki, Efthimios Sivridis, Karl Morten, Ioannis Roxanis, Russell Leek, Francesca Buffa, Michael Koukourakis, Adrian L. Harris
Journal: Cancer Research (2015)

Abstract The microtubule-associated protein light chain 3 MAP1LC3 (LC3) and GABARAPL1 and 2 are the major proteins to monitor autophagy. Here, we analyzed the role of both MAP1LC3, GABARAPL1 and 2 in breast cancer cells and primary human breast cancer. HER2 amplified (BT474, SKBR3, MDA-MB-453), ER positive (MCF7, T47D) and triple negative (MDAMB231) breast cancer cell lines were studied in response to hypoxia, in high [25mM] and low glucose [5mM] treatment. Low glucose increased the basal levels for LC3A-II, LC3B-II and GABARAPL2-II in BT474, MCF7 and MDAMB231 cell lines. SKBR3 cell line had the lowest autophagic protein levels for LC3B-II, and Gabarapl2-II and was the most resistance to the autophagy inhibition mediated using siRNAs to all theseIn hypoxia both LC3B (up to 8 fold) and GABARAPL1 (up to 12 fold) mRNA expression were induced in all cell lines (p&amp;lt;0.001). MAP1LC3A knockdown significantly decrease cell proliferation in BT474, MCF7 and MDAMB231 while GABARAPL2 knockdown additionally decreased baseline cell proliferation in T47D and SKBR3 breast cancer cells (p&amp;lt;0.05, n = 3). Moreover, using the Seahorse, decreased MAP1LC3A and GABARAPL2 expression by siRNA led to cellular bioenergetic changes including increased basal oxygen consumption of the MCF7. Co-expression analysis of autophagic genes define distinctive clusters of MAP1LC3, GABARAPL 1 and 2 with the hypoxic signature in breast cancer subtypes using the METABRIC series. The expression levels of the MAP1LC3 and GABARAPL1 and 2 among the breast cancer subtypes and normal tissue showed significant suppession of all of them in cancer compared to normal breast tissue. In conclusion, all the pathways were suppressed at RNA level in cancer compared to normal, baseline levels were not associated with core classification of breast cancer types. These latter data were confirmed in a breast cancer cell line panel, which showed a marked variation in inducibility by environemntal stress and role in survival under low glucose and hypoxia. Thus analysis of induced gene expression in response to stress may be important, or heterogeneity in selection of tumors for anti-autophagy therapy. Citation Format: Christos E. Zois, Syed Haider, Simon Wigfield, Alexandra Giatromanolaki, Efthimios Sivridis, Karl Morten, Ioannis Roxanis, Russell Leek, Francesca Buffa, Michael Koukourakis, Adrian L. Harris. Differential regulation of LC3 A and B, GABARAPL 1 and 2 autophagy genes by micro-environmental stress and role in breast cancer survival. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2903. doi:10.1158/1538-7445.AM2015-2903
Modeling BCR-ABL and MLL-AF9 leukemia in a human bone marrow-like scaffold-based xenograft model
Authors: P Sontakke, M Carretta, J Jaques, A Z Brouwers-Vos, L Lubbers-Aalders, H Yuan, J D de Bruijn, A C M Martens, E Vellenga, R W J Groen, J J Schuringa
Journal: Leukemia (2016)
Two Photon Intravital Microscopy Reveals CD4+ T Cells Making Cognate Interactions With Tissue Dendritic Cells In Skin Graft-Versus-Host-Disease
Authors: Sarah Morin-Zorman, Christian Wysocki, Edwina Kisanga, David Gonzalez, David Rothstein, Fadi Lakkis, Ann Haberman, Warren D Shlomchik
Journal: Blood (2013)
Adjuvant chemoradiotherapy with carboplatin and a fluoropyrimidine for resectable gastric and gastroesophageal junction cancer: A retrospective review of the Stanford experience
Authors: M. A. de Bruin, P. L. Kunz, V. B. Sharma, J. A. Norton, J. Bastidas, D. T. Chang, A. C. Koong, A. C. Koong, R. R. Balise, J. M. Ford, G. A. Fisher
Journal: Journal of Clinical Oncology (2009)

e15674 Background: The standard of care for the adjuvant treatment of resected gastric or gastroesophageal junction (GEJ) adenocarcinoma in the U.S. is post-operative 5FU and radiotherapy per the MacDonald regimen. At Stanford Cancer Center (SCC) we have adopted a modified regimen of chemoradiotherapy using carboplatin and a fluoropyrimidine. Methods: A retrospective review was performed of patients at SCC with T2-T4 or node positive gastric or GEJ cancer who underwent surgery with curative intent, and then received the following treatment. Carboplatin (AUC 6) was administered on days 1 and 22. Patients also received either 5FU at 200 mg/m2/day (via continuous infusion) plus leucovorin for six weeks, or capecitabine at 1,000 mg/po BID for 14 days, repeated every 21 days for 2 cycles. At week 8, infusional 5FU or capecitabine was combined with external beam radiotherapy to the gastric bed for five weeks (total 4,500 cGy). At week 14, patients repeated an additional 2 cycles of carboplatin and fluoropyrimidine as tolerated. Results: Forty-nine patients were identified. The majority (76%) were male. Median age at diagnosis was 57 years. Thirty-nine had gastric and 10 had GEJ cancers. With a mean follow up of 35 months, twenty-one patients (43%) have died; median disease free and overall survival have not been reached. Eighteen patients (37%) have recurred. The percentage of patients alive by stage was 100% (4/4) for stage IB, 62% (8/13) for stage 2, 53% (9/17) for stage IIIA, 33% (2/6) for stage IIIB and 22% (2/9) for stage IV. Grade 3 or 4 toxicities occurred in 27 patients (55%); most common were neutropenia (16), thrombocytopenia (11) and gastrointestinal toxicity in (8). Conclusions: Adjuvant chemoradiotherapy with carboplatin and a fluoropyrimidine after curative resection of gastric and GEJ cancer was well tolerated and yielded survival results similar to historical data. No significant financial relationships to disclose.
PTH-200 Amino acid profiles in patient with intestinal failure: preliminary data on biochemical insights
Authors: KC Fragkos, M Samaan, S Morgan, I Barnova, L Smith, M Babington, PS Patel, N Keane, F Rahman, S Di Caro
Journal: Gut (2015)

<h3>Introduction</h3> Amino acid profiles have become increasingly important in intestinal failure (IF), ever since citrulline has been used as an IF marker. The purpose of the present study is to investigate levels in patients with IF needing parenteral nutrition (PN) and to examine their use as markers of IF severity to guide clinical decisions. <h3>Method</h3> Serum amino acids levels were measured prospectively within the cohort of patients managed by the IF Unit at our hospital using Ion Exchange Chromatography. Statistical analysis was performed by R and SPSS 21.0 using Pearson’s Correlation coefficient, t-tests and ANOVA. The traditional classification of IF<sup>1</sup>was taken as a proxy of IF severity where Type 1 IF was coded as 1 and type 3 IF was coded as 3. <h3>Results</h3> Our study included 35 patients (11 Males, mean age 45, range 16–87), non IF controls (n = 10), Type 1 IF (n = 3), Type 2 IF (n = 3), Type 3 IF (n = 19); all IF patients were on PN. Causes of IF were short bowel syndrome, enterocutaneous fistula, high stoma output, pseudo-obstruction and radiation enteropathy. Citrulline was significantly lower in patients with Type 3 IF (p = 0.033) and receiving PN (p = 0.031) compared to other groups. Valine was significantly lower in patients with Type 3 and 2 IF compared to other controls and type 1 (p = 0.053); a trend was observed for lower homocysteine in patients receiving PN (p = 0.082). The other amino acids didn’t display differences with respects to groups. Figure 1 shows the correlation matrix between amino acids levels and severity of IF. Lower levels of citrulline, valine, alanine, total homocysteine, methionine, histidine, ornithine, and isoleucine were associated with more severe IF (range of correlation coefficients –0.432 to –0.174) whilst glutamate levels had a positive association (0.215) with less severe IF. Strong positive associations were found between amino acids valine, histidine, threonine, methionine, ornithine, alanine, leucine and isoleucine (correlation coefficient range 0.41–0.73), suggesting involvement in common biochemical pathways. <h3>Conclusion</h3> The present study confirmed the usefulness of citrulline as a marker of enterocytes mass in IF It provided new evidence that lower levels of valine, total homocysteine, methionine, histidine, ornithine, and isoleucine were associated with more severe IF. Larger studies are needed to validate those data. <h3>Disclosure of interest</h3> None Declared. <h3>Reference</h3> ESPEN endorsed recommendations. Definition and classification of intestinal failure in adults. <i>Clin Nutr</i>. 2014. doi: 10.1016/j.clnu.2014.08.017
Surface‐Driven Collagen Self‐Assembly Affects Early Osteogenic Stem Cell Signaling
Authors: Tojo Razafiarison, Unai Silván, Daniela Meier, Jess G. Snedeker
Journal: Advanced Healthcare Materials (2016)

This study reports how extracellular matrix (ECM) ligand self‐assembly on biomaterial surfaces and the resulting nanoscale architecture can drive stem cell behavior. To isolate the biological effects of surface wettability on protein deposition, folding, and ligand activity, a polydimethylsiloxane (PDMS)‐based platform was developed and characterized with the ability to tune wettability of elastomeric substrates with otherwise equivalent topology, ligand loading, and mechanical properties. Using this platform, markedly different assembly of covalently bound type I collagen monomers was observed depending on wettability, with hydrophobic substrates yielding a relatively rough layer of collagen aggregates compared to a smooth collagen layer on more hydrophilic substrates. Cellular and molecular investigations with human bone marrow stromal cells revealed higher osteogenic differentiation and upregulation of focal adhesion‐related components on the resulting smooth collagen layer coated substrates. The initial collagen assembly driven by the PDMS surface directly affected α1β1 integrin/discoidin domain receptor 1 signaling, activation of the extracellular signal‐regulated kinase/mitogen activated protein kinase pathway, and ultimately markers of osteogenic stem cell differentiation. We demonstrate for the first time that surface‐driven ligand assembly on material surfaces, even on materials with otherwise identical starting topographies and mechanical properties, can dominate the biomaterial surface‐driven cell response.
The critical role of the central nervous system (pro)renin receptor in regulating systemic blood pressure
Authors: Quanbin Xu, Dane D. Jensen, Hua Peng, Yumei Feng
Journal: Pharmacology & Therapeutics (2016)

The systemic renin–angiotensin system (RAS) has long been recognized as a critically important system in blood pressure (BP) regulation. However, extensive evidence has shown that a majority of RAS components are also present in many tissues and play indispensable roles in BP regulation. Here, we review evidence that RAS components, notably including the newly identified (pro)renin receptor (PRR), are present in the brain and are essential for the central regulation of BP. Binding of the PRR to its ligand, prorenin or renin, increases BP and promotes progression of cardiovascular diseases in an angiotensin II-dependent and -independent manner, establishing the PRR a promising antihypertensive drug target. We also review the existing PRR blockers, including handle region peptide and PRO20, and propose a rationale for blocking prorenin/PRR activation as a therapeutic approach that does not affect the actions of the PRR in vacuolar H+-ATPase and development. Finally, we summarize categories of currently available antihypertensive drugs and consider future perspectives.
IL-1β, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
Authors: Suzanne M Bal, Jochem H Bernink, Maho Nagasawa, Jelle Groot, Medya M Shikhagaie, Kornel Golebski, Cornelis M van Drunen, Rene Lutter, Rene E Jonkers, Pleun Hombrink, Melanie Bruchard, Julien Villaudy, J Marius Munneke, Wytske Fokkens, Jonas S Erjefält, Hergen Spits, Xavier Romero Ros
Journal: Nature Immunology (2016)
TCTAP A-078 The Effect of Admission Blood Glucose and Hemoglobin A1C on Prognosis of Diabetic Patients with Acute Myocardial Infarction: From KorMI Registry
Authors: KangUn Choi, Byung-Jun Kim, Jong-Ho Nam, Kyu-Hwan Park, Chan-Hee Lee, Jang-Won Son, Ung Kim, Jong-Seon Park, Dong-Gu Shin, Young-Jo Kim, Jun-Ho Bae, Deuk-Young Nah, Jeong-Hwan Cho, Sang-Wook Kang
Journal: Journal of the American College of Cardiology (2016)
‘FIFA 11 for Health’ for Europe. II: effect on health markers and physical fitness in Danish schoolchildren aged 10–12 years
Authors: Christina Ørntoft, Colin W Fuller, Malte Nejst Larsen, Jens Bangsbo, Jiri Dvorak, Peter Krustrup
Journal: British Journal of Sports Medicine (2016)

Objectives To evaluate whether a modified ‘FIFA 11 for Health’ programme for non-communicable diseases had effects on body composition, blood pressure and physical fitness of Danish schoolchildren aged 10–12 years. Design A cluster-randomised controlled study with 7 intervention and 2 control schools. Participants 546 Danish 5th grade municipal schoolchildren allocated to an intervention group (IG; n=402: 11.1±0.4 (±SD) years, 150.1±7.0 cm, 41.3±8.4 kg) and a control group (CG; n=144: 11.0±0.5 years, 151.2±7.8 cm, 41.3±9.0 kg). Intervention As part of the physical education (PE) curriculum, IG carried out 2 weekly 45 min ‘FIFA 11 for Health’ sessions focusing on health issues, football skills and 3v3 games. CG continued regular school PE activities. Measurements of body composition, blood pressure at rest, Yo-Yo intermittent recovery level 1 children's test (YYIR1C), balance, jump and sprint performance were performed before and after the 11-week study period. Results During the 11-week study period, systolic blood pressure (−3.5 vs 0.9 mm Hg), mean arterial blood pressure (−1.9 vs 0.4 mm Hg), body mass index (−0.02 vs 0.13 kg/m 2 ) and body fat percentage (−0.83% vs −0.04%) decreased more (p&lt;0.05) in IG than in CG. Within-group improvements (p&lt;0.05) were observed in IG for 20 m sprint (4.09±0.29 to 4.06±0.28 s) and YYIR1C performance (852±464 to 896±517 m), but these changes were not significantly different from CG, and balance or jump performance remained unchanged in both groups. Conclusions The modified ‘FIFA 11 for Health’ programme has beneficial effects on body composition and blood pressure for Danish schoolchildren aged 10–12 years, thereby providing evidence that this football-based health education programme can directly impact participants' cardiovascular health profile.
TCTAP C-168 In-stent Chronic Total Occlusion at Left Anterior Descending Artery Treated by AXXESS Dedicated Bifurcation Stent and Additional Drug-eluting Stents: The First Case of AXXESS-treated Chronic Total Occlusion in Taiwan
Authors: Chao-Feng Lin, Tsung-Yeh Yang, Chun Chao Chen
Journal: Journal of the American College of Cardiology (2016)
Acclimation during space flight: effects on human emotion
Authors: Qing Liu, Ren-Lai Zhou, Xin Zhao, Xiao-Ping Chen, Shan-Guang Chen
Journal: Military Medical Research (2016)

Recently, studies on the extent to which spaceflight affects the psychology of individuals has received attention. In order to reveal the mental challenges that humans face in space, we need practical viewpoints to integrate the psychological effects, behavior, performance and the environment itself for space exploration. The present review discusses the individual variables related to space psychology and manned spaceflight, in addition to their growing trends. These items include patterns of emotional changes in extreme environments and the approaches to evaluating emotions. Moreover, the review concludes with suggested future research on emotion during spaceflight and its analogs. These data and information are needed to plan for the exploration of the Moon and Mars, along with contributions to the construction of the international space station (ISS) and astronaut training.
Antiangiogenic activity of clofarabine
Authors: Anshu M. Roy, Rongbao Li, Zhican Qu
Journal: Cancer Research (2006)

235  2-chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)adenine (clofarabine) is a new-generation nucleoside analog discovered by Southern Research Institute based on the experience with cladribine and fludarabrine. Clofarabine has demonstrated significant single-agent antitumor activity and has recently been approved by the FDA for the treatment of pediatric patients with relapsed or refractory acute lymphoblast leukemia. Clofarabine has also shown significant efficacy in xenograft models of solid tumors; its activity in solid tumors is presently being investigated clinically. It is known that clofarabine inhibits DNA synthesis and repair and induces apoptosis by disrupting the integrity of mitochondrial membranes. However, it has not been tested if clofarabine is active against tumor angiogenesis that is required for a solid tumor to grow beyond 2 mm in diameter. In this study, we have investigated the effect of clofarabine on endothelial cell proliferation, migration and differentiation into capillary vessels, the key steps in angiogenesis. The results show that clofarabine inhibits human endothelial cell proliferation, with an IC50 value in sub-micromolar range, and also their migration towards the angiogenic factors, an important functional property of endothelial cells to form the vascular network. Clofarabine, also, clearly interrupts the endothelial cell differentiation to form endothelial tubes. These results indicate that clofarabine is not only active against tumor cell growth, but also active against endothelial cells to inhibit its proliferation, migration and microvessel tube formation, suggesting an important role of clofarabine in antiangiogenesis. Further studies on the mechanism by which clofarabine inhibits neoangiogenesis are being undertaken. These findings highlight the potential of clofarabine as an effective agent against human solid tumors, which combines anti-proliferative and antiangiogenic activities in one molecule, and provide information to further optimize dosage and sequence of clofarabine treatment in future clinical trials.
Accupedo pedometer: daily walking step counter
Authors: Zoran Milanović, Nenad Stojiljković, Ljubomir Pavlović, Vladimir Antić, Nemanja Stanković
Journal: British Journal of Sports Medicine (2016)

iOS (requires iOS 8.1 or later), Android (versions 2.2 or later)

Lite version—free; Accupedo-Pro Pedometer $3.99

Accupedo, a pedometer app, monitors daily walking and calculates the physical activity level. With more than 5 million downloads and 3.8 overall reviewers score, this is one of the most popular apps in the health and fitness category. The accuracy of this app is based on three-dimensional (3D) motion recognition algorithms which track walking patterns by filtering and rejecting non-walking activities. Normally an ‘intelligent algorithm’ starts tracking after 10 consecutive steps, then stops and restarts automatically as you walk, but the advantage of this app compared to the other commercial 2D sensor pedometers is Accupedo's ability to count steps regardless of where you put your …