tbuckley/cabot-search huggingface dataset:
Abstract 1609: Functional analysis of SFRS10: A novel molecular target for anti-cancer drug
Authors: Satoru Wada, Hidetaka Eguchi, Keiji Tanimoto, Keiko Hiyama, Masahiko Nishiyama
Journal: Cancer Research (2010)

Abstract [Background] Development of novel molecular-target drugs is actively conducted: A large number of such drugs are under clinical trials. However, an ideal molecular-target drug with high-specificity and low-toxicity is yet to be developed. For epoch-making development of cancer drugs, it is necessary to further identify target candidates by unprecedented approach. [Purpose] Last year, we reported 13 candidate genes that contributed to an immortalization through comprehensive gene expression analysis of human mortal- and immortal-cells and tissues. Under the same procedure, we here added SFRS10 gene to the list. To evaluate its possibility as drug target, we attempted here a functional analysis of SFRS10. [Materials and Methods] Human colon cancer cell lines DLD-1 and HCC48 and pancreatic cancer cells MIAPaCa-2, KLM-1, and BxPC-3 were used in each experiment. Quantitative analyses of gene expression levels were conducted by real-time RT-PCR method using gene specific-primers and Universal Probe Library probes (Roche). Microarray analysis was conducted with 4×44K Whole Human Genome Microarray (Agilent). Pathway analysis was performed through the use of Ingenuity Pathways Analysis (IPA, Ingenuity® Systems, www.ingenuity.com). Knockdown experiments were performed using Stealth RNAi and RNAiMax reagent (Invitrogen). Cell proliferation was measured with MTT assay. Caspase activities were analyzed using ApoAlert Caspase Profiling Plate (Clontech). Cell cycle profile was obtained by measuring PI-stained cells with FACS “Calibur” (BD Biosciences). [Results] SFRS10 knockdown induced inhibition of cell proliferation of all the tested cells. Because knockdown efficiencies and cytostatic activities showed a good correlation in the experiment, we speculated that SFRS10 may have an important role for the cell proliferation. Treatment with SFRS10 siRNA induced cell cycle arrest in the S and G2/M phases and apoptosis in DLD-1 and KLM-1 cells. Moreover, we observed that some cell cycle-related genes, such as MAD2L1 and RFC4, altered their expression levels in response to SFRS10 knockdown as assessed by microarray analysis; cell cycle checkpoint pathways were significantly changed with network analyses. The alteration of gene expression levels were confirmed by real-time RT-PCR analyses. [Conclusion] These results suggested that SFRS10 may have an important role in regulation of cell proliferation and may control cell cycle checkpoints. Taken together, SFRS10 may be one molecule responsible for immortalization of cancer cells and the useful molecular target for cancer therapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1609.
Elastogenesis in the Vaginal Wall and Pelvic-Organ Prolapse
Authors: Kathleen A. Connell
Journal: New England Journal of Medicine (2011)

Pelvic-organ prolapse is a debilitating condition that affects millions of women and is associated with costs of more than $1 billion annually in the United States alone.1 The recurrence of pelvic-organ prolapse after the various surgical treatment options is nearly 30%, with declining success with subsequent procedures.2 Although alterations in collagen, elastin, and proteoglycan proteins of the extracellular matrix within the pelvic-support ligaments and vaginal tissue have been associated with pelvic-organ prolapse, our understanding of the underlying causative mechanisms are lacking, which limits approaches to treatment. A recent study by Budatha and colleagues3 sheds light on the mechanistic role of . . .
ABL gene fuses BCR during t(20;22) results in Philadelphia-negative, but BCR/ABL-positive chronic myeloid leukemia
Authors: Y Ito, T Shimamoto, Y Kuriyama, A Nakajima, M Sumi, Y Watanabe, H Beppu, K Ohtsubo, K Ohyashiki
Journal: Leukemia (2002)
SAT0187 Immunomodulatory effects of human umbilical cord wharton jelly-derived mesenchymal stem cells (HUCMS) on circulating T-cell subsets in patients with sjögren’s syndrome
Authors: A. Alunno, P. Montanucci, O. Bistoni, G. Basta, S. Caterbi, T. Pescara, E. Bartoloni, I. Pennoni, F. Cannarile, R. Calafiore, R. Gerli
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> hUCMS are adult stem cells easy to retrieve in bulk. Their immunomodulatory and pro-differentiation properties has been demonstrated. Indeed they may suppress lymphocyte activation, thereby prolonging skin graft survival time. hUCMS may also promote differentiation of tolerizing dendritic cells which favors development of Treg. These properties seem to derive both from hUCMS-secreted soluble factors such as TGFβ and on contact with the target cell. Very few evidences are currently available regarding potential therapeutic application of hUCMS in systemic autoimmune disorders and in particular no data have been published in Sjogren's syndrome (SS) to date. <h3>Objectives</h3> We aimed to assess hUCMS immunomodulatory in vitro effects on circulating SS-T cells. <h3>Methods</h3> hUCMS were isolated from umbilical cords according to the method that we recently developed and published (1). Five SS patients and 5 healthy donors (HD) were enrolled. Peripheral blood mononuclear cells (PBMC) were obtained by density gradient from SS and HD heparinized venous blood. In selected experiments CD3+ cells were isolated from PBMC by magnetic sorting prior to arrange cultures. Fluorescein diacetate and ethidium bromide and trypan blue dye exclusion assays were performed to assess cell viability. Lymphocytes proliferation was assessed by CFSE diluition assay and culture supernatants were tested for IL-17 with commercial ELISA kit. Intracellular IL-17 was performed by flow cytometry following 6 hours stimulation with PMA, ionomycin and brefeldin. <h3>Results</h3> hUCMS and PBMC were successfully co-cultured in CMRL 1066 medium with 10% fetal bovine serum (FBS). When PBMC were used, cell proliferation was dramatically inhibited by hUCMS when co-culture was performed in contact. Interestingly, the higher was PBMC:hUCMS ratio the stronger was such inhibition. IL-17 concentration in supernatants and the percentage of CD4+IL17+ cells were significantly reduced by hUCMS. When magnetic sorted CD3+ cells were used, the proliferation rate was not affected and the effect on IL-17 concentration in supernatants and the percentage of CD4+IL17+ cells was partially lost. Co-cultures in transwell and with microencapsulated hUCMS are currently ongoing. <h3>Conclusions</h3> Our preliminary results suggest possible therapeutic applications of hUCMS by selectively targeting Th17 pathogenic cells in SS. <h3>References</h3> Montanucci P et al. Tissue Eng Part A 2011 Montanucci P. et al. Biomaterials 2011;32:9254 <h3>Disclosure of Interest</h3> None Declared
Long-acting recombinant coagulation factor IX albumin fusion protein (rIX-FP) in hemophilia B: results of a phase 3 trial
Authors: P. Saiag, R. Gutzmer, P. Ascierto, M. Maio, J.-J. Grob, P. Murawa, B. Dréno, M. Ross, J. Weber, A. Hauschild, P. Rutkowski, A. Testori, E. Levchenko, A. Enk, L. Misery, C. Vanden Abeele, I. Vojtek, O. Peeters, V. Brichard, P. Therasse, Anne Samier-Guérin, Alain Saraux, Simon Gestin, Divi Cornec, Thierry Marhadour, Valérie Devauchelle-Pensec, Luc Bressollette, Michel Nonent, Sandrine Jousse-Joulin, Sébastien Sanges, David Launay, Rennie Rhee, Olivier Sitbon, Eric Hachulla, Luc Mouthon, Loïc Guillevin, Laurence Rottat, David Montani, Pascal de Groote, Vincent Cottin, Pascal Magro, Gregoire Prevot, Fabrice Bauer, Emmanuel Bergot, Céline Chabanne, Martine Reynaud-Gaubert, Sylvie Leroy, Matthieu Canuet, Olivier Sanchez, Christophe Gut-Gobert, Claire Dauphin, Christophe Pison, Clement Boissin, Gilbert Habib, Pierre Clerson, François Conesa, François Cordier, Steven Kawut, Gerald Simonneau, Marc Humbert, Elena Santagostino, Uri Martinowitz, Toshko Lissitchkov, Brigitte Pan-Petesch, Hideji Hanabusa, Johannes Oldenburg, Lisa Boggio, Claude Negrier, Ingrid Pabinger, Mario von Depka Prondzinski, Carmen Altisent, Giancarlo Castaman, Koji Yamamoto, Maria-Teresa Álvarez-Roman, Christine Voigt, Nicole Blackman, Iris Jacobs
Journal: Blood (2016)
Esophageal submucosal dissection under steady pressure automatically controlled endoscopy (SPACE): a clinical feasibility study
Authors: Motohiko Kato, Kiyokazu Nakajima, Takuya Yamada, Masashi Hirota, Yasuaki Miyazaki, Makoto Yamasaki, Tsutomu Nishida, Masaki Mori, Yuichiro Doki, Masahiko Tsujii, Tetsuo Takehara
Journal: Endoscopy (2014)

<b>Background and study aims:</b> Steady pressure automatically controlled endoscopy (SPACE) is a new insufflation system that provides constant carbon dioxide (CO<sub>2</sub>) insufflation pressure during prolonged procedures. The system consists of an overtube, a surgical insufflator, and a newly developed leak-proof valve. The aims of this study were to validate the feasibility and safety of SPACE for esophageal endoscopic submucosal dissection (ESD). <b>Patients and methods:</b> This was a clinical phase I trial, involving 10 patients who underwent esophageal ESD. The primary end point was the rate of adverse events within 30 days (grade 0 to 4). Secondary end points were changes in partial pressure of carbon dioxide (PaCO<sub>2</sub>) and vital signs during ESD, completion rate of ESD, and degree of abdominal distension by patient assessment and radiographic grading. <b>Results: </b>All adverse events were Grade 2 or less. Mild PaCO<sub>2 </sub>elevation after ESD was noted; however, no associated symptoms were reported. The procedure was completed under SPACE alone in 8 of 10 patients. Minimal post-procedural bowel distension was observed. <b>Conclusions:</b> In this small pilot study, SPACE was feasible and appeared to be safe. Further study with larger case numbers is required to demonstrate efficacy and safety. Clinical trial registration: UMIN000005434
Abstract 1905: The association between coffee and green tea intake and upper aerodigestive tract cancer in Japanese population
Authors: Isao Oze, Keitaro Matsuo, Satoyo Hosono, Hidemi Ito, Miki Watanabe, Kazuo Tajima, Hideo Tanaka
Journal: Cancer Research (2011)

Abstract [Background] Coffee and tea are the most common beverages worldwide. Specifically, green tea is the most common tea in Japan. Coffee and green tea are potentially thought to have anticancer effect because of the antioxidative components such as chlorogenic acids in coffee and catechins in tea. On the other hand, coffee and green tea are often consumed with high temperature. Hot foods and beverages are considered as risk factor for esophageal cancer (e.g. yarba mate in Latin America). Thus, coffee and green tea might be carcinogenic. Then, we conducted a case-control study to elucidate the association between UAT cancer and coffee and green tea consumption. [Method] Cases were 961 patients with histologically diagnosed as UAT cancer (head and neck cancer in 527 and esophageal cancer in 434) between January 2001 and December 2005 at Aichi Cancer Center Hospital. Controls were 2883 first-visit outpatients who were confirmed to have no cancer during the same period. Cases and Controls were matched by age and sex. Information on coffee and green tea consumption and other lifestyle factors were obtained via self-administered questionnaire. Associations between coffee and green tea consumption and UAT cancer were assessed by odds ratios (ORs) and 95% confidence intervals (CIs) by using conditional logistic regression models. [Result] Both coffee and green tea consumption had significant association with UAT cancer overall (trend p=0.011 and 0.007, respectively). Those who had three or more cups of coffee a day showed significantly lower OR for UAT cancer (OR 0.71, 95% CI 0.55-0.93) than those with non-daily consumers. In contrast, three or more cups of green tea consumption had significant positive association with UAT cancer (OR 1.28, 95% CI 1.06-1.56). These trends were not clear for esophageal cancer. The inverse association between coffee consumption and UAT cancer was evident among current drinkers, while the positive association between green tea and UAT cancer was evident among never drinkers. Similar trends were observed in other categories. [Conclusion] UAT cancer was inversely associated with coffee consumption, and positively associated with green tea consumption. Further epidemiologic studies are warranted. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1905. doi:10.1158/1538-7445.AM2011-1905
Authors’ response: Measles outbreak linked to an international dog show in Slovenia, 2014
Authors: M Grgič-Vitek, None on behalf of the authors of the original article
Journal: Eurosurveillance (2015)

Preview this article: Authors’ response: Measles outbreak linked to an international dog show in Slovenia, 2014, Page 1 of 1 < Previous page | Next page > /docserver/preview/fulltext/eurosurveillance/20/9/art21052-en-1.gif
Interpandemic Influenza in the Houston Area, 1974–76
Authors: W. Paul Glezen, Robert B. Couch, Robert A. MacLean, Ardath Payne, Justus N. Baird, Carlos Vallbona, Michael Tristan, Nancy Byrd
Journal: New England Journal of Medicine (1978)

Prospective virologic surveillance has defined two influenza epidemics representing the fifth and sixth outbreaks attributed to H3N2 viruses since the prototype, A/Hong Kong/68 (H3N2), emerged in 1968. The 1975 epidemic was caused by influenza A/ Port Chalmers and yielded an estimated attack rate of 9 per cent; the second, attributed to influenza A/Victoria, produced an explosive outbreak, with an estimated attack rate of 18 per cent in 1976. The highest morbidity occurred in preschool children, with an estimated attack rate of over 30 per cent. During the early stages of both epidemics there was a predominance of cases among school-aged children, and school absenteeism peaked earlier than other nonvirologic indexes. These observations support the concept of rapid dissemination of influenza among schoolchildren and suggest that control of epidemic influenza might be facilitated by prophylaxis for that age group and other accessible, healthy populations. (N Engl J Med 298:587–592, 1978)
Case 16-2007
Authors: Cameron D. Wright, Panos Fidias, Noah C.H. Choi, Jo-Anne O. Shepard, Robert P. Hasserjian
Journal: New England Journal of Medicine (2007)

A 61-year-old man was referred to the thoracic oncology service for management of a thymoma. Six weeks earlier, a mediastinal mass, 4 cm in diameter, had been detected on a computed tomographic scan obtained because of chest pain. A positron-emission tomographic scan showed increased tracer uptake in the area of the lesion. Biopsy specimens showed a World Health Organization type B1 thymoma. Physical examination was normal. A decision on management was made.
Abstract 3072: Characterization of TCL1-Tg:p53-/- mice that resemble human chronic lymphocytic leukemia with 17p-deletion: alterations in p53→Mir30→EZH2 axis.
Authors: Jinyun Liu, Marcia A. Ogasawara, Gang Chen, Helene Pelicano, Peng Huang
Journal: Cancer Research (2013)

Abstract Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in the United States and Europe. CLL patients with deletion of chromosome 17p, where the tumor suppressor p53 gene is located, often develop a more aggressive disease with poor clinical outcomes. However, the underlying mechanism remains unclear. We have recently generated mice with Eμ-Tcl1-Tg:p53-/- genotype and showed that these mice develop aggressive leukemia that resembles human CLL with 17p deletion. In the present study, we further demonstrated that the p53 deficiency in the Tcl1 transgenic mice resulted in significant down-regulation of microRNAs miR-15a and miR16-1, associated with a substantial up-regulation of Mcl-1, suggesting that the p53-miR15a/16-Mcl-1 axis may play an important role in CLL pathogenesis. Interestingly, we also found that loss of p53 resulted in a significant decrease in expression of the miR-30 family in leukemia lymphocytes from the Eμ-Tcl1-Tg:p53-/- mice. Consistently, primary leukemia cells from CLL patients with 17p deletion also showed a decrease in the miR-30d expression. To further exam the biological significance of decrease in the miR-30 family in CLL, we investigated the potential involvement of EZH2 (enhancer of zeste homolog 2), a component of the Polycomb repressive complex known to be a downstream target of miR-30d and plays a role in disease progression in several solid cancers. RT-PCR and western blot analyses showed that both EZH2 mRNA transcript and protein levels were significantly increased in the lymphocytes of Eμ-Tcl1-Tg:p53-/- mice relative to Eμ-Tcl1-Tg:p53+/+ mice. Exposure of leukemia cells isolated from Eμ-Tcl1-Tg:p53-/- mice to the EZH2 inhibitor 3-deazaneplanocin (DZNep) led to induction of apoptosis, suggesting EZH2 may play a role in promoting CLL cell survival and this may contribute to the aggressive phenotype of CLL with loss of p53. Our study reveals that the p53-miR30-EZH2 axis may play an important role in CLL pathogenesis, and EZH2 may potentially be a target for CLL treatment. Citation Format: Jinyun Liu, Marcia A. Ogasawara, Gang Chen, Helene Pelicano, Peng Huang. Characterization of TCL1-Tg:p53-/- mice that resemble human chronic lymphocytic leukemia with 17p-deletion: alterations in p53→Mir30→EZH2 axis. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3072. doi:10.1158/1538-7445.AM2013-3072
AB0978 Comparison of effectiveness and safety of tramadol/acetaminophen and non-steroidal anti-inflammatory drugs (NSAIDS) for treatment of knee osteoarthritis in elderly patients
Authors: J.-W. Hur, J.W. Ryou, S.Y. Kim
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The use of a combination of oral analgesics vs. an individual agent offers several potential benefits. Combining analgesics into a single product may facilitate prescribing and compliance by reducing the total number of medications that a patient must take to manage pain. Furthermore elderly patients usually have risk of bleeding and other complications. <h3>Objectives</h3> To compare the analgesic effectiveness and safety of tramadol 37.5mg/acetaminophen 325mg combination tablets(tramadol/acetaminophen) with non-steroidal anti-inflammatory drugs (NSAIDs) for treatment of osteoarthrits in elderly patients. <h3>Methods</h3> This randomized controlled study enrolled 48 patients with chronic knee osteoarthritis that diagnostic period was over 3 months. Patients with at least moderate pain [visual analog scale (VAS) with scores ≥40/100 mm] after washout period were randomized to tramadol/acetaminophen or NSAID. All patients received tramadol/acetaminophen 1 tablet TID or NSAID (naproxen 500mg) BID. Primary outcome measure was final pain VAS score. Secondary measures included pain relief (5 point Likert scale), adverse events, and overall medication assessments. <h3>Results</h3> In total, 43 intent-to-treat (ITT) patients received tramadol/acetaminophen (n=21) or NSAID (n=22). Mean baseline pain VAS scores were not difference between two groups. ITT analysis showed significantly better mean final VAS scores (38.45 vs 31.24, p=0.004) and mean final pain relief scores (2.18 vs 2.95; p=0.007) for tramadol/acetaminophen than for NSAID. The most common treatment related adverse events with tramadol/acetaminophen were nausea/vomiting (47.6%) and constipation (33.3%). Epigastric soreness and heartburn was more frequent in NSAID treatment. <h3>Conclusions</h3> Tramadol/acetaminophen combination showed better effectiveness in pain reduction compared with NSAIDs used for the treatment of osteoarthritis in elderly patients. <h3>References</h3> Tramadol/acetaminophen combination tablets and codeine/acetaminophen combination capsules for the management of chronic pain: A comparative trial. Clinical Therapeutics. 2001;23:1429-45. Efficacy and safety of tramadol HCl in breakthrough musculoskeletal pain attributed to osteoarthritis. J Rheumatol. 1998;25:1358-63. <h3>Disclosure of Interest</h3> None Declared
The Value of Ga-67 Scintigraphy in Sarcoid Myopathy
Authors: TIBERIU HERSHCOVICI, SERGEI MEKHMANDOROV, YITZHAK BEIGEL, RUTH HARDOFF
Journal: Clinical Nuclear Medicine (2001)

HERSHCOVICI, TIBERIU M.D.; MEKHMANDOROV, SERGEI M.D.; BEIGEL, YITZHAK M.D.; HARDOFF, RUTH M.D., D.Sc. Author Information
PMO-210 Double balloon enteroscopy: how useful is it to confirm Crohn's disease?: Abstract PMO-210 Table 1
Authors: C Parker, R Perowne, D Nylander, S Panter
Journal: Gut (2012)

<h3>Introduction</h3> Double Balloon Enteroscopy (DBE) is widely used in clinical practice worldwide. A DBE service at South Tyneside District Hospital was commenced in January 2010 to complement the existing capsule endoscopy (CE) service. We present the results of an audit of prior investigation before DBE. <h3>Methods</h3> Clinical records were examined for patients referred for DBE with a diagnosis of suspected Crohn9s disease. Information was gathered regarding: place of referral, previous imaging and endoscopy, findings and histology. <h3>Results</h3> 28/37 (77%) of referrals were from outside our hospital. 15/37 referred for investigation of Crohn9s disease, 75% of these were from outside our hospital. Seven patients with known Crohn9s were referred for investigation of recurrent symptoms or for possible stricturing disease. Eight patients were referred with possible Crohn9s based on clinical symptoms and signs. All patients had been previously investigated with multiple endoscopic or imaging modalities. Most common method of prior imaging for patients being investigated for Crohn9s disease was Barium follow through (BaFT) 42%, followed by CE 33%, CT 12.5%, MRE 12.5%. 87% had a colonoscopy prior to referral. 11/15 had abnormal imaging, 5 (33.3%) having inflammatory changes seen on CE. Of these histology was taken in three and found: Crohn9s (1), non-specific inflammation (1), normal (1). 3/5 cases were normal at DBE. 4/15 had entirely normal previous investigations. Of the eight patients with suspected Crohn9s, two patients with abnormal radiology had DBE findings consistent with Crohn9s. Of the three patients with SB ulceration on CE only one had an abnormal DBE and histology obtained was inconclusive. See Abstract PMO-210 table 1. <h3>Conclusion</h3> A large proportion of our referrals are to investigate Crohn9s disease. Most have had multiple endoscopic and imaging modalities prior to referral. DBE is more likely to have positive findings when associated with abnormal imaging rather than abnormal VCE findings. Only those with abnormalities on imaging other than VCE were confirmed to have Crohn9s disease; it may be that subtle inflammatory changes on VCE are over reported or that findings were beyond the reach of DBE. Our figures although small would suggest that in those with normal radiological imaging there is little improvement in diagnostic yield with DBE. <h3>Competing interests</h3> None declared.