tbuckley/cabot-search huggingface dataset:
Abstract 3712: 2-Methoxyestradiol inhibits Barrett's esophageal adenocarcinoma growth and aggressive phenotypes through differential regulation of β-catenin-E-cadherin-axis
Authors: Suman Kambhampati, Snigdha Banerjee, Kakali Dhar, Smita Mehta, Inamul Haque, Gopal Dhar, Monami Majumder, Gibanananda Ray, Peter J. Vanveldhuizen, Sushanta K. Banerjee
Journal: Cancer Research (2010)

Abstract The purpose of this study was to evaluate whether 2-methoxyestradiol (2-ME2), a promising anticancer agent, modulates Barrett's esophageal adenocarcinoma (BEAC) cell growth and behavior through cellular pathway involving beta-catenin in partnership with E-cadherin, which appears to play a critical role in the induction of antitumor responses in cancer cells. We found that 2-ME2 markedly reduced the BEAC cell proliferation via regulating apoptotic machinery such as Bcl-2 and Bax. It may nullify the aggressive behavior of the cells by reducing the migratory behavior. Expressions of beta-catenin and E-cadherin and binding of these two proteins is activated in a 2-ME2-dependent fashion in Bic-1 cells. Moreover, over expressions of these two proteins may be due to the stabilization of these proteins by 2-ME2. We found that 2-ME2-induced anti-migratory effects are mediated through the beta-catenin -E-cadherin signaling pathways. In view of these results, we determined whether 2-ME2 reduces BEAC tumor growth. Administration of 2-ME2 significantly decreased the growth of BEAC cells xenografted on the flank of nude mice. The evidence presented points out that the impact of 2-ME2 on beta-catenin-orchestrated signal transduction plausibly plays a multi-faceted functional role to inhibit the proliferation and cell migration of 2-ME2 treated malignant cells and it could be a potential candidate in novel treatment strategies for Barrett's esophageal adenocarcinoma Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3712.
Extraventricular Neurocytoma Treated With 177Lu DOTATATE PRRT Induction and Maintenance Therapies
Authors: William Makis, Karey McCann, Alexander J.B. McEwan
Journal: Clinical Nuclear Medicine (2015)

A 64-year-old woman with a 30-year history of recurrent sellar masses presented with severe headache and rapidly progressive visual field loss in the left eye. She was diagnosed with an extraventricular neurocytoma, which was octreotide positive, and was referred for PRRT (peptide receptor radionuclide therapy) with 177Lu [DOTA0,Tyr3]octreotate (DOTATATE). After 4 induction and 4 maintenance treatment cycles, her headaches resolved, her vision improved, and her sellar mass stabilized. The use of 177Lu DOTATATE PRRT in the management of extraventricular neurocytoma warrants further research.
Comparison of the diagnostic value of miniprobe sonography system and conventional EUS combined with fine-needle aspiration for gastric lymphoma
Authors: Sun Yun-wei, Gong Ting-ting, Zuo Li-ping, Zhu Qi
Journal: Endoscopy (2011)

Aim: To evaluate and compare the potential value of MPS, conventional EUS and EUS-FNA for diagnosis of gastric lymphoma during the follow-up investigations with treatment of radiation or chemotherapy.
Abstract 2449: Pancreatic cancer tumor initiating cells are marked by the presence of a primary cilium
Authors: Jennifer M. Bailey, Zeshaan Rasheed, William Matsui, Anirban Maitra, Michael A. Hollingsworth, Steven D. Leach
Journal: Cancer Research (2011)

Abstract Primary cilia are microtubule rich projections eminating from the apical surface of epithelial cells. Primary cilia are responsible for the transduction of growth factor and morphogenetic signaling pathways, including the hedgehog, wnt and platelet-derived growth factor pathways. Initial observations in the field of pancreatic cancer have suggested the absence of primary cilia in human pancreatic intraepithelial neoplasia (PanIN) and pancreatic ductal adenocarcinoma. While absent in the majority of pancreatic cancer cells, primary cilia have been identified in differentiated acinar cells undergoing an acinar to ductal metaplasia. This metaplasia is well characterized in pancreatic injury and regeneration models and accelerates tumor formation in the presence of acinar specific KrasG12D mutations. Thus, we are addressing the hypothesis that primary cilia, while not expressed in bulk tumor populations, are maintained in pancreatic tumor-initiating cells. To address the hypothesis, we have used confocal microscopy to quantify the percentage of cells within low grade human PanIN lesions that express a primary cilium. Performing confocal microscopy on a human PanIN tissue array (n=23), we have determined that in 15% of PanIN lesions, 13-30% of the cells are expressing a primary cilium, as is evidenced by staining for acetylated alpha tubulin. We have also developed a sorting strategy using an acetylated alpha tubulin antibody to isolate ciliated cells by Fluorescence Activated Cell Sorting (FACS) analysis. Using this protocol, we have discovered that human pancreatic cancer cells bearing a primary cilium are markedly enriched in cells also positive for either CD133 or CD24/CD44, representing putative pancreatic cancer stem cell populations. We have functionally analyzed the tumor-initiating capacity of ciliated vs non-ciliated cells using tumor sphere experiments. In this assay, ciliated cells show a significant increase (100 fold) in tumor sphere-forming capacity, suggesting that the presence of a primary cilium may mark a pancreatic cancer tumor initiating population. These data are informative regarding the earliest initiating events in pancreatic cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2449. doi:10.1158/1538-7445.AM2011-2449
Fatal mediastinitis following botulinum toxin injection for esophageal spasm
Authors: Sophie Marjoux, Mathieu Pioche, Thomas Benet, Jean-Sébastien Lanne, Sabine Roman, Thierry Ponchon, François Mion
Journal: Endoscopy (2013)

Endoscopic injection of botulinum toxin (BTX) may be used to treat achalasia or distal esophageal spasm [1] [2] [3]. We report a case of fatal mediastinitis following this treatment.
ASSOCIATION OF MAJOR AND MINOR ELECTROCARDIOGRAPHIC ABNORMALITIES WITH CARDIOVASCULAR MORBIDITY AND MORTALITY IN THE MULTI-ETHNIC STUDY OF ATHEROSCLEROSIS COHORT
Authors: Panteha Rezaeian, David N. Ezekiel, Thomas Diaz, Roger Blumenthal, Christine Darwin, Robyn McClelland, Donald Lloyd-Jones, Romeo Castillo, Hobart Lee, Khurram Nasir, Saman Nazarian, Juan Rivera, Elsayed Soliman, Matthew Budoff
Journal: Journal of the American College of Cardiology (2016)
AB0126 CD70 Expressing CD4 T Cells Produce IFN-Γ and IL-17 in Rheumatoid Arthritis
Authors: J.K. Park, B.K. Han, J.A. Park, Y.J. Woo, S.Y. Kim, E.Y. Lee, E.B. Lee, P. Chalan, A.M. Boots, Y.W. Song
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> CD70 expressing CD4 T cells are enriched in rheumatoid arthritis (RA) and promote autoimmunity via costimulatory CD70-CD27 interaction. To date, it is unknown whether CD70+ CD4 T cells contribute to RA pathogenesis <i>via</i> other mechanisms than the CD70-CD27 interaction. <h3>Objectives</h3> To explore the phenotype and cytokine production of CD70+ CD4 T cells in RA. <h3>Methods</h3> Peripheral blood mononuclear cells (PBMCs) from 32 RA patients were isolated and frequencies of CD70+ cells within different CD4 T subsets were analyzed using flow cytometry. Interferon (IFN)-γ and interleukin (IL)-17 production were compared between the CD70+ and CD70- cells. Expression of master transcription factors T-bet, GATA3 and retinoic-acid-related orphan receptor gamma t (RORγt) were examined by real time-PCR. Results were in mean ± standard error of the mean. <h3>Results</h3> CD4 T cells of healthy controls rarely expressed CD70 as compared to CD4 T cells of RA patients (0.9±0.3% vs. 7.6±0.6%, p&lt;0.001, panel A). In RA, CD70+ cells were present within all CD4 T cell subsets, i.e. CD45RA+ CCR7+ naïve, CD45RA-CCR7+ central memory, CD45RA-CCR7- effector memory, and CD45RA+ CCR7- terminally differentiated effector memory T cells with a frequency of 3.9±1.1%, 4.0±0.5%, 4.2±0.7%, and 9.4±4.3%, respectively (panel B). As compared to CD70- CD4 T cells, CD70+ CD4 T cells produced significantly more IFN-γ (panel C) and IL-17 (panel D) after short activation. CD70+ CD4 T cells preferentially expressed transcription factor RORγt. <h3>Conclusions</h3> CD70+ CD4 T cells are enriched in RA and may directly contribute to RA pathogenesis by producing IFN-γ and IL-17. Targeting CD70+ CD4 T cells might offer new therapeutic opportunities in RA. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2780
Abstract 5137: A genome wide RNAi screen identify STAT3 activation as essential for ErbB2 mediated transforamtion of breast cancer cells
Authors: Ruth Rodriguez Barrueco
Journal: Cancer Research (2012)

Abstract The generation of multidimensional datasets characterizing genetic, epigenetic, and functional properties of large normal and tumor-related samples is creating unique opportunities for the systems-level dissection of mechanisms associated with malignant phenotypes. Integrated data sets may lead to more efficient and specific approaches for the elucidation of therapeutic targets and biomarkers and for the identification of specific small molecule inhibitors. However, the power of these computational methods to study human cancer from an unbiased global perspective is severely compromised by the low through-put approaches necessary to functionally validate the in silico predictions. During the last years, RNA interference (RNAi) emerged as a very powerful genetic strategy to functionally interrogate the entire genome by loss-of-function studies. This cellular process can be experimentally controlled to knock down the expression of any specific gene. Our group has pioneered the development of RNAi-based genetic tools to translate RNAi studies to mammalian systems generating a shRNA-mir pooled lentiviral library that allows performing functional studies at genome-wide level. ErbB2 is a receptor tyrosine kinase found overexpressed in 20-40% of breast tumors correlating with a malignant phenotype and worse prognosis. Different targeted therapies have been developed to specifically inhibit its activity such the monoclonal antibody Herceptin, or the small tyrosine kinase inhibitor Lapatinib. Despite of the success of these inhibitors in the treatment of tumors that overexpress ErbB2, about 30% of them become resistant making necessary to look for alternative targets in these cases. The combination of the state-of-the-art computational analysis and RNAi-based technology toward system biology studies must represent a powerful tool to dissect the regulatory networks that govern the homeostasis of breast tumors. Applying this combined approach to an overexpressing ErbB2 breast cancer model we have identified the genes that are critical in those cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5137. doi:1538-7445.AM2012-5137
1103 A 4 SERUM PROTEIN SIGNATURE FOR THE DIAGNOSIS OF MILD FIBROSIS IN CHRONIC HEPATITIS C (HEPACHRONIX STUDY)
Authors: B. Jardin-Watelet, I. Bieche, M. Lapalus, A. Duces, E. Mathieu- Dupas, S. De Muynck, N. Lambert, I. Baxtelli-Molina, B. Sallenave, E. Nicolas, E. Estrabaud, N. Jullian, J. Balicchi, M. Martinot-Peignoux, O. Lada, V. Paradis, D. Valla, P. Bedossa, D. Laune, P. Marcellin, M. Vidaud, T. Asselah
Journal: Journal of Hepatology (2013)
Endoscopic Ultrasonography in Patients with Thyroid Cancer: Its Usefulness and Limitations for Evaluating Esophagopharyngeal Invasion
Authors: E. Koike, H. Yamashita, S. Noguchi, A. Ohshima, H. Yamashita, S. Watanabe, S. Uchino, T. Arita, S. Kuroki, M. Tanaka
Journal: Endoscopy (2002)

Although computed tomography (CT) and magnetic resonance imaging (MRI) are useful for detecting esophagopharyngeal invasion by thyroid cancer, they cannot assess the depth of invasion, which is important for the surgical planning. In the present study, endoscopic ultrasonography (EUS) of the esophagus was used to assess esophagopharyngeal invasion by thyroid cancer, and the value of this technique was assessed prospectively.Between 1 December 1998 and 31 December 2000, EUS examinations were carried out in 59 patients in whom esophagopharyngeal invasion by thyroid cancer was suspected due to large tumors or tumors with poor mobility. The EUS findings were evaluated in 52 patients (10 men, 42 women; mean age 62.4 years; mean tumor size 39.5mm) with complete resections, and compared with the pathological results.The diagnostic specificity and accuracy of EUS in assessing invasion into the muscularis propria (82.9 %, 82.7 %) were greater than those of MRI (60 %; P = 0.034, 65.4 %; P = 0.044) and esophagography (58.8 %; P = 0.034, 60 %; P = 0.028). The accuracy for detecting cancer invasion located in the upper part of the lobe was less than for invasion in the middle and/or lower parts of the lobe ( P = 0.020). Conclusions. EUS is useful for assessing esophagopharyngeal invasion by thyroid cancer, although its effectiveness may be limited in thyroid lesions located in the upper part of the lobe.
Abstract 39: A mathematical model for the dependence on tumor size of the rate of metastasis formation
Authors: Ardith W. El-Kareh, Timothy W. Secomb
Journal: Cancer Research (2011)

Abstract That the dependence of the rate of formation of new metastases on the existing tumor volume is weak has been demonstrated from data on the distribution of sizes of metastasic lesions, as well as data on the fraction of tumors that are metastatic as a function of tumor size at diagnosis. A mathematical model for the rate of metastasis formation as a function of tumor size was developed to explain this finding and is presented here. The model includes several stromal cell populations, in particular macrophages and fibroblasts, in addition to tumor cells, which are divided into epithelial- and mesenchymal-like populations. Epithelial-to-mesenchymal transitions are accounted for, as well as key growth factors that mediate tumor-stroma crosstalk and recruitment of stromal cells to the tumor mass. A key feature of the model is cooperativity in the steps involved in the establishment of metastases. Experimental findings of tumor cell-macrophage cooperativity in chemotaxis-driven migration towards microvessels, and EMT/non-EMT cooperativity in tumor cell escape, extravasation and intravasation are incorporated into the model. By involving the product of two or more rates, each of which depend on tumor volume raised to a power less than one, these cooperative processes result in a dependence of the metastatic rate on tumor size that is less than the 2/3 power expected if the rate were merely surface-area dependent. In addition to providing a framework for understanding experimental findings on the kinetics of metastasis, the model should prove useful for investigating the interplay between cell kill rates due to treatment and tumor regrowth kinetics in the most common case where the treated tumor consists of a distribution of metastatic lesions rather than a single mass. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 39. doi:10.1158/1538-7445.AM2011-39
Clinical Determinants of the Racial Disparity in Very Low Birth Weight
Authors: Allison Kempe, Paul H. Wise, Susan E. Barkan, William M. Sappenfield, Benjamin Sachs, Steven L. Gortmaker, Arthur M. Sobol, Lewis R. First, DeWayne Pursley, Heidi Rinehart, Milton Kotelchuck, F. Sessions Cole, Nita Gunter, Joseph W. Stockbauer
Journal: New England Journal of Medicine (1992)

Although the risk of very low birth weight (<1500 g) is more than twice as high among blacks as among whites in the United States, the clinical conditions associated with this disparity remain poorly explored.
OC-017 A Discriminant Analysis Demonstrates that Siblings of Patients with Crohn’S Disease have a Distinct Microbiological and Immune Phenotype Compared with Healthy Controls: Insights into Disease Pathogenesis
Authors: C Hedin, N E McCarthy, P Louis, F Farquharson, S McCartney, K Taylor, N Prescott, T Murrells, A J Stagg, K Whelan, J O Lindsay
Journal: Gut (2013)

<h3>Introduction</h3> Crohn’s disease (CD) is associated with genetic risk, intestinal dysbiosis, altered blood T-cell phenotype, increased faecal calprotectin (FC) and intestinal permeability (IP). Factors shared by CD patients and unaffected siblings may be implicated in CD pathogenesis. <h3>Aims</h3> Delineate the genetic, immune and microbial phenotype of patients, siblings and healthy controls (HC); identify factors associated with CD that discriminate siblings from HC. <h3>Methods</h3> Faecal microbiota, FC, blood T-cell phenotype, IP and genotype risk over 72 CD risk loci, were measured by qPCR, ELISA, flow cytometry, sugar permeability and Illumina Bead Array respectively, in 22 patients with inactive CD, 21 of their healthy siblings and 25 HC. <h3>Results</h3> In addition to genotype risk, siblings shared aspects of the phenotype of CD patients, distinct from HC, as previously reported.1 Direct discriminant function analysis revealed that the variables maximally separating siblings from HC (Function 2) were: increased β7 integrin expression by circulating naïve CD4+ T-cells and an increased proportion of memory CD4+ T-cells as well as reduced faecal <i>Roseburia</i> spp. (Image 1). In contrast, the variables differentiating CD patients from HC (Function 1) were: elevated FC and altered faecal microbiota (reduced <i>Faecalibacterium prausnitzii</i>, Cluster IV<i> Ruminococcus spp.</i><i>Bacteroides-Prevotella and</i> Clostridial cluster IV). <h3>Conclusion</h3> Healthy siblings of CD patients manifest immune and microbiological abnormalities associated with CD, distinct from their genetic risk. Unaffected siblings of CD patients are an excellent model in which to investigate early CD pathogenesis. <h3>Disclosure of Interest</h3> None Declared <h3>Reference</h3> Hedin <i>et al.</i><i></i> Gut 2012; 61: Suppl. 2 A22-A23.
The “U-wire” technique during ERCP for easy guide wire passage through occluded uncovered metal stents
Authors: F. Liu, J. Zhu, Z. Li
Journal: Endoscopy (2012)

A significant number of self-expanding metal stents (SEMSs) placed as conservative therapy for malignant biliary obstruction will become occluded [1]. Stent occlusion can be treated endoscopically either by balloon trawling to remove the biliary sludge and debris or by inserting a polyethylene stent or a second stent through the occluded stent [2]. However, these treatments can be given only after a guide wire is passed through the occluded lumen of the stent into the bile duct beyond [1], and not infrequently during endoscopic retrograde cholangiopancreatography (ERCP), a guide wire accidentally passes through the mesh of an uncovered metal stent, which may result in prolongation of the ERCP procedure or fluoroscopy.
Dideoxyinosine in Children with Symptomatic Human Immunodeficiency Virus Infection
Authors: Karina M. Butler, Robert N. Husson, Frank M. Balis, Pim Brouwers, Janie Eddy, Deborah El-Amin, Janet Gress, Mary Hawkins, Paul Jarosinski, Howard Moss, David Poplack, Sheila Santacroce, David Venzon, Lori Wiener, Pamela Wolters, A. Pizzo Philip
Journal: New England Journal of Medicine (1991)

2′,3′-Dideoxyinosine (ddI) is a dideoxynucleoside with potent activity in vitro against the human immunodeficiency virus (HIV). In initial clinical trials in adults, ddI showed evidence of antiretroviral activity with little hematologic toxicity.
Case 28-2007
Authors: James L. Januzzi, Joseph M. Garasic, Tomas G. Neilan, R. Gilberto Gonzalez, James R. Stone
Journal: New England Journal of Medicine (2007)

A 68-year-old man was admitted to the hospital because of a syncopal episode, followed by hemiparesis and altered mental status. An electrocardiogram revealed ST-segment elevations in the anterolateral leads, and MRI of the brain revealed infarcts involving all vascular distributions. Transthoracic echocardiography showed hypokinesis of the left ventricle and a mobile echodensity in the left atrium. Angiography revealed occlusion of the left anterior descending coronary artery, both renal arteries, and the superior mesenteric artery. A diagnostic procedure was performed.
P208 IFNL4 STIMULATES EXPRESSION OF IFNλS AND DIFFERENTIALLY CONTROLS BASAL EXPRESSION LEVELS OF ANTI-VIRAL ISGS AND SIGNAL SUPPRESSORS WHICH LEAD TO DIFFERENT EFFECTS OF IFN
Authors: H. Abe, C.N. Hayes, D. Miki, N. Hiraga, M. Imamura, M. Tsuge, T. Kobayashi, Y. Kawakami, H. Aikata, H. Ochi, K. Chayama
Journal: Journal of Hepatology (2014)
Role of SPECT/CT in Sentinel Lymph Node Detection in Patients With Breast Cancer
Authors: Laetitia Vercellino, Jessica Ohnona, David Groheux, Abdelhak Slama, Patrick M. Colletti, Sotirios Chondrogiannis, Pascal Merlet, Marie-Elisabeth Toubert, Domenico Rubello
Journal: Clinical Nuclear Medicine (2013)

Aim The purpose of this study was to conduct a systematic review of the published literature to assess the role (indications, advantages, and limitations) of SPECT/CT for the detection of sentinel lymph node (SLN) in breast cancer. Methods The authors searched PubMed for published literature in English addressing this topic. Results Eleven studies, published since 2006, focused on the role and value of SPECT/CT for SLN detection (SLND). They showed that SPECT/CT improved sentinel node detection and anatomical localization. One study suggested that SPECT/CT may provide a more accurate staging. Limitations for SLND with SPECT/CT include extra time and inconvenience for the patient and additional radiation dose. Conclusions SPECT/CT is a valuable tool for SLND, especially in difficult cases, when planar lymphoscintigraphy shows no SLN or unexpected lymphatic drainage.
EFFECT OF CANAGLIFLOZIN ON TRIGLYCERIDE-TO-HIGH DENSITY LIPOPROTEIN CHOLESTEROL RATIO, A MARKER OF INSULIN RESISTANCE IN PATIENTS WITH TYPE 2 DIABETES MELLITUS
Authors: Kathleen L. Wyne, Anita Ziboh, Ujjwala Vijapurkar, Michael Davies, Gary Meininger
Journal: Journal of the American College of Cardiology (2015)
Characterization and Significance of Sulfonylurea Receptors
Authors: Linda Siconolfi-Baez, Mary Ann Banerji, Harold E Lebovitz
Journal: Diabetes Care (1990)

This study describes and characterizes a putative sulfonylurea receptor. The radioligand used was [3H]glipizide (9 Ci/mmol). The beta-cell plasma membranes were derived from a transplantable rat insulinoma generated by subcutaneous injection of RINm5F cells and purified by ultracentrifugation on a 15-55% sucrose gradient. Specific binding of [3H]glipizide to purified beta-cell plasma membranes was determined to be maximal at temperatures of 4-23 degrees C, pH 7.3, and an incubation of 2 h. Scatchard analysis indicated a single binding site with Kd = 7 nM and sulfonylurea binding of 0.93 pmol/mg membrane protein. Displacement of [3H]glipizide from the purified beta-cell plasma membranes by various sulfonylureas and their analogues correlated well with their known hypoglycemic and insulin-releasing activities. Various agents, including nutrients, agents affecting Ca2+ flux, gastrointestinal hormones, and pancreatic hormones, had no effect on [3H]glipizide binding to the beta-cell plasma membranes. Putative sulfonylurea receptors on beta-cell and brain cell plasma membranes have been reported by several groups of investigators. Sulfonylurea binding to the beta-cell is hypothesized to close an ATP-sensitive K+ channel, which leads to depolarization of the membrane and activation of a voltage-dependent Ca2+ channel.