tbuckley/cabot-search huggingface dataset:
AB1378 Prevalence and associated factors of chronic widespread pain among the attendees of general practice in sri lanka
Authors: P.V. De Silva
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Chronic widespread pain (CWP) is a common health problem within the community and among the working population [1]. This has been identified as one of the most common complains encountered in general practice and in rheumatology clinics. However, this was not properly studied among the attendees of general practice in Sri Lanka. <h3>Objectives</h3> This study was carried out to identify the prevalence and associated factors of CWP among the attendees of general practice <h3>Methods</h3> All the adults (above 30 years) who were attended to a general practice in a rural area of Galle district during January 2011 were included in the study. The general practitioner collected the data and diagnosis of CWP was made using the "Manchester definition" for CWP. It defined CWP as "<i>pain reported in at least two sections of two contralateral limbs and in the axial skeleton, and have been present for at least three months"</i>. <h3>Results</h3> Out of the 620 eligible participants only 556 participated in the survey with a non response rate of 10.3%. Out of the participants' majority were (57.9%) females. Mean age was 54.4 years. Prevalence of the CWP among females (13.0%) was higher compared to the prevalence among males (8.5%). Overall prevalence was 11.1%. Female gender, age above 60 years, low socio-economic status, low education status and having co-morbidities were associated with the CWP. <h3>Conclusions</h3> Although it is a highly neglected problem in Sri Lanka, more than 10% of the adult population attending to a general practice is suffering from CWP. Therefore, general practitioners have to be more vigilant about the diagnosis and the management of CWP. <h3>References</h3> Croft PR, Rigby PS, Boswell R, Schollum J, Silman AJ. The prevalence of chronic widespread pain in the general population. J Rheumatol 1993;14:41-45. <h3>Disclosure of Interest</h3> None Declared
Tc-99m Leukocyte Imaging for Evaluating Disease Severity and Monitoring Treatment Response in Ulcerative Colitis: Comparison With Colonoscopy
Authors: TAMIO ABURANO, YASUHIRO SAITO, NORIYUKI SHUKE, TOKIYOSHI AYABE, YUTAKA KOHGO, JUNICHI SATO, YUKIO ISHIKAWA
Journal: Clinical Nuclear Medicine (1998)

The diagnostic value of Tc-99m leukocyte imaging for evaluating disease activity and monitoring treatment response in ulcerative colitis was compared with that of colonoscopy. A total of 48 studies were performed in 28 patients with ulcerative colitis. Forty-six studies had both Tc-99m leukocyte imaging and colonoscopy within 2 weeks of each other, and the remaining two studies had only Tc-99m leukocyte imaging. In 46 studies, semiquantitative grading of colonic radioactivity by comparison with iliac crest marrow activity on radionuclide imaging correlated well with macroscopic grading based on Matts' criteria on colonoscopy. Fourteen (82%) of 17 studies with slight colonoscopic change showed a negative result on the radionuclide image. In 15 patients treated with glucocorticoids or leukapheresis, radionuclide imaging showed nearly the same statistical results as colonoscopy in monitoring treatment response. Tc-99m leukocyte imaging could be used as an alternative to colonoscopy to evaluate the disease activity of ulcerative colitis or to monitor the treatment response, although it is insensitive for detecting disease with only slight macroscopic changes.
P113 DEFICIENT MICRORNA-21 INDUCTION PROMOTES SMALL-FOR-SIZE SYNDROME FOLLOWING EXTENDED HEPATECTOMY IN MICE
Authors: E. Kachaylo, C. Tschuor, P. Limani, K. Lehmann, P. Ramadori, M. Foti, R. Graf, B. Humar, P.-A. Clavien
Journal: Journal of Hepatology (2014)
Shiitake mushroom-induced ileus managed using double-balloon enteroscopy
Authors: Meiko Shozushima, Ken Ohata, Kouichi Nonaka, Nobuyuki Matsuhashi
Journal: Endoscopy (2013)

Detection of the cause of dietetic ileus is often difficult. High quality computed tomography (CT) scanning has improved our ability to precisely diagnose a large variety of intra-abdominal and intraluminal disease processes, including the presence of foreign bodies [1]. Here we report a case of ileus caused by ingestion of shiitake mushroom that could be diagnosed before treatment and could be treated endoscopically without surgery.
Abstract 2959: The Syk tyrosine kinase negatively affects cell cycle progression through phosphorylation of the Cdk1 kinase in response to DNA damage
Authors: Michael Saen, Anne Morel, Romain Larive, Serge Urbach, Patrick Jouin, May C. Morris, Vjekoslav Dulic, Paul Mangeat, Peter J. Coopman
Journal: Cancer Research (2011)

Abstract The non-receptor spleen tyrosine kinase Syk has mainly been studied in hematopoietic cells in which it plays a key role in the immune-response signalling. We previously demonstrated that Syk is also present in mammary epithelial cells and that its expression is lost during malignant progression of breast cancer cell lines. Clinical studies confirmed loss of Syk expression, not only in breast tumors but also in other carcinomas and melanoma. Xenotransplantation animal experiments evidenced that Syk acts as a tumor and metastasis suppressor in breast cancer and melanoma. The mechanisms by which Syk exerts its anti-oncogenic activity remain, however, unrevealed. We previously demonstrated that Syk localizes to the centrosomes and negatively affects cell division. The centrosomal Syk substrates and possible effectors of its anti-oncogenic activity remain, nevertheless, unknown. In this study, we demonstrate that Syk may negatively affect cell cycle progression through the phosphorylation of the Cdk1 kinase, a key protein involved in the control of mitosis. Using immunofluorescence, we observed that endogenous Syk and Cdk1 colocalize at the centrosomes and that transient DsRed-Syk transfection induces pY15-Cdk1-positive epitopes at the centrosomes. In vitro kinase assays demonstrate that Syk can phosphorylate Cdk1 in the presence of different cyclins. Mass spectrometry allowed identification of four different Cdk1 tyrosine residues phosphorylated by Syk, amongst which the Y15 is known to negatively affect mitotic entry when phosphorylated by the Wee1 kinase. Transient overexpression of DsRed-Syk or GFP-Cdk1 carrying phospho-mimicking mutations resulted in a cell cycle block as observed by FACS analysis and led to a senescence-like growth arrest. Finally, we demonstrate that DNA damage-inducing agents induce Syk activation and Cdk1 tyrosine phosphorylation in a Syk-dependent manner, as evidenced by the use of a Syk-targeting shRNA. In conclusion, our results reveal that Syk overexpression or Syk activation by genotoxic agents induce a cell cycle arrest through the phosphorylation of Cdk1. These novel aspects of Syk function will contribute to a better understanding of its onco-suppressive activity and, more generally, of the DNA damage-induced signalling. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2959. doi:10.1158/1538-7445.AM2011-2959
Abstract 3640: Increased anti-tumor effects of sequential docetaxel followed by 5-FU treatment against human oral cancer cells
Authors: Tetsuya Tamatani, Tarannum Ferdous, Daisuke Uchida, Hirokazu Nagai, Youji Miyamoto
Journal: Cancer Research (2010)

Abstract [Background] Docetaxel (TXT), a novel member of the taxoid family, and 5-fluorouracil (5-FU) are key drugs against oral cancer chemotherapy. The promising clinical activity of TXT and 5-FU has promoted considerable interest in combining these drugs. Although the combination treatment has been reported to improve the response rates, sequential treatment with TXT and 5-FU was unknown. [Purpose] The aim of this study was to evaluate the anti-tumor effects of sequential treatment with TXT and 5-FU against oral squamous cell carcinoma cells. [Methods and Results] Two human oral squamous cell carcinoma cell lines, B88 and CAL27 cells were used in this study. The effects of sequential treatment on the inhibition of tumor growth with TXT and 5-FU were investigated by 3-(4, 5-dimethylthiaol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, and the xenograft on nude mice. In vivo study, B88 or CAL cells (5×106) were subcutaneously inoculated into the backs of nude mice. When the tumor reached 0.5 cm in diameter, tumor-bearing nude mice received either the intraperitoneal administration of TXT (10 mg/kg) alone, 5-FU (15 mg/kg) alone, TXT followed by 5-FU or 5-FU followed by TXT treatment. TXT followed by 5-FU treatment more effectively inhibited the tumor growth in vitro and in vivo, and induced apoptosis than 5-FU followed by TXT treatment. To elucidate the mechanisms underlying growth inhibitory effect of TXT followed by 5-FU, we examined the expression of 5-FU metabolic enzymes, thymidylate synthase (TS), dehydropyrimidine dehydrogenase (DPD), and ortate phosphorybosyl transferase (OPRT) by real-time PCR and western blot analysis. Down-regulation of TS and DPD expression, and up-regulation of OPRT expression were induced by TXT treatment compared with 5-FU, suggesting that they enhanced the efficacy of 5-FU. [Conclusions]These findings demonstrated that sequential treatment with TXT followed by 5-FU can be effective for the patients with oral squamous cell carcinoma. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3640.
P2-18-01: The Magnitude of Trastuzumab Benefit in HER2−Positive (HER2+) Lobular Breast Carcinoma (BC): Results of a HERA Trial Sub-Group Analysis.
Authors: O Metzger, M Procter, Azambuja E de, G Viale, B Leyland-Jones, M Dowsett, R Gelber, E Gresko, S Loi, C Sotiriou, M Piccart
Journal: Cancer Research (2011)

Abstract Background: Invasive lobular carcinoma (ILC) represents the second most common BC subtype and is often characterized as hormone receptor positive and HER2−negative. However a small subset of ILC is found to be HER2+. Isolated case reports have demonstrated high responsiveness to trastuzumab in patients with advanced HER2+ ILC. The HERA trial compares 1 or 2 years of trastuzumab treatment with observation and 1 versus 2 years of trastuzumab treatment after standard chemotherapy in women with HER2+ breast cancer. We sought to evaluate the incidence of HER2+ ILC and the magnitude of trastuzumab benefit in HER2+ ILC in the context of the HERA trial. In addition, we sought to describe the pattern of hormone receptor positivity in the subsets of ILC and invasive ductal carcinoma (IDC) Methods: The database used in the analysis had a clinical cut-off date of 9th June 2008 and 4-year median follow-up (Gianni et al., 2011). Patients randomized to the 1-year trastuzumab and observation arms were included in the present analysis. Central assessment of hormone receptor status was considered. Histological BC subtype was assessed locally. Results: Of the 1703 women randomized to one-year of trastuzumab and 1698 to observation, 5.5% (n=187) and 94.5% (n=3213) were diagnosed as HER2+ ILC and IDC, respectively. Central hormone receptor status was available in 88.3% (n = 2838) of IDC and 86.1% (n=161) of ILC. ER and/or PR positivity was more common in ILC than IDC (63.4% [102/161] vs. 46.3% [1314/2838]; p&amp;lt;0.001). Allred scores for ER are shown in the table below. The DFS hazard ratios comparing one year of trastuzumab versus observation were 0.63 (95% CI 0.34−1.14) for ILC and 0.77 (95% CI 0.67−0.89) for IDC. There was no evidence of an interaction between histological subtype and trastuzumab benefit (interaction [subtype lobular and subtype ductal and not lobular] p=0.49). Conclusion: HER2+ ILC accounts for 5.5% of patients included in a large population of over 3,000 HER2+ BC. While only a limited number of patients with ILC was enrolled, this analysis suggests an increased ER positivity in ILC compared to IDC. There was no suggestion that patients with HER2+ ILC derived a different magnitude of benefit from adjuvant trastuzumab when compared to the HER2+ IDC cohort. The lack of central pathology review for BC subtype assessment is a caveat of our study. Future research in the field of ILC and particularly in the HER2+ subset should be encouraged. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P2-18-01.
Intérêt de l'endomicroscopie confocale dans le dépistage de la dysplasie sur gastrite chronique atrophique
Authors: D Moussata, L Senouci, M Chauvenet, F Berger, K Stroeymeyt, G Boschetti, S Nancey, JC Saurin, B Flourié
Journal: Endoscopy (2012)

De diagnostic tardif, le cancer de l'estomac représente la deuxième cause de décès par cancers digestifs (survie à 5 ans <20%). Le cancer est précédé d'une gastrite chronique atrophique (GCA) qui peut progresser vers la métaplasie intestinale puis la dysplasie. Pour faciliter le dépistage de la dysplasie, une cartografie avec chromoendoscopie (CE) est recommandée, mais elle manque de sensibilité. L'endomicroscopie confocale (EMC), permet de réaliser un diagnostic histologique pendant l'endoscopie et de mieux cibler les biopsies. But: Evaluer l'intérêt de l' EMC dans le dépistage de la dysplasie en comparant le taux de dysplasie détectée par CE seule et par CE couplée à l'EMC (CE+EMC) selon une cartografie bien établie.
PTH-107 Knowledge of South Asian Population in Inflammatory Bowel Disease: Ccknow- How much do they Know?
Authors: S Soteriadou, S Hickman, J K Limdi
Journal: Gut (2013)

<h3>Introduction</h3> Despite an unprecedented explosion in information and technology and improved understanding of the aetiopathogenesis of Inflammatory bowel diseases (IBD) it remains unclear whether this has translated into a meaningful improvement in patients understanding of their disease. Less is known about South Asian patients with IBD. Our aim was to assess the understanding amongst South Asian patient’s using the validated CCKNOW questionnaire<sup>1</sup>. <h3>Methods</h3> We conducted a prospective questionnaire-based study of 108 South Asian IBD patients at our institution. Patient demographics, first language, country of origin, educational level and disease duration were recorded. A previously validated 24-item Crohn’s and Colitis Knowledge (CCKNOW) questionnaire was used to assess IBD-specific knowledge relating to disease1. <h3>Results</h3> Forty-three of 108 surveys sent out were returned (39.8%). Twenty-three of the 43 patients surveyed were male (53.5%). Fifteen were born in the UK (34.8%). English was recorded as the first language in 15 (34.9%). The highest education level was high school comprehensive in 19 (44.1%), sixth-form/technical college in 5 (11.6%), university in 14 (32.6%), and postgraduate in 3 (6.98%). Fifty one percent of patients described their health to be average or below average, as opposed to good. Both the mean and median CCKNOW score were 6.74 (Range 0–20). Mean scores related to anatomy were 1.21 (n = 5; 24.1%), aetiology 0.77 (n = 2; 38.35%), symptoms 1.55 (n = 3; 38.7%), diagnosis 0.19 (n = 1; 18.6%), treatment and related complications 1.91 (n = 7; 23.6%), surgery 0.07 (n = 1, 7%), complications 0.93 (n = 4; 23.2%), and pregnancy and fertility related questions 0.12 (n = 1; 11.6%) <h3>Conclusion</h3> The knowledge base of South Asian IBD patients was thus deemed poor. More concerning were specific areas of deficient knowledge such as pregnancy and fertility, cancer and treatment related complications with the potential for serious consequences. <h3>Patient</h3> education is a key determinant of clinician –patient relationship and an important outcome modifier in chronic disease. IBD teams must guide information needs of specific patient groups through well designed material to enhance knowledge and understanding of disease ultimately impacting on quality of life, treatment adherence and reduction in the use of healthcare resources <h3>Disclosure of Interest</h3> None Declared. <h3>Reference</h3> Eaden JA, Abrams K, Mayberry JF. The Crohn’s and Colitis Knowledge Score: a test for measuring patient knowledge in inflammatory bowel disease. Am J Gastroenterol. 1999 Dec; 94(12):3560–6.
The Role of TNF Superfamily Member 13 in the Progression of IgA Nephropathy
Authors: Seung Seok Han, Seung Hee Yang, Murim Choi, Hang-Rae Kim, Kwangsoo Kim, Sangmoon Lee, Kyung Chul Moon, Joo Young Kim, Hajeong Lee, Jung Pyo Lee, Ji Yong Jung, Sejoong Kim, Kwon Wook Joo, Chun Soo Lim, Shin-Wook Kang, Yon Su Kim, Dong Ki Kim
Journal: Journal of the American Society of Nephrology (2016)

TNF superfamily member 13 (TNFSF13) has been identified as a susceptibility gene for IgA nephropathy in recent genetic studies. However, the role of TNFSF13 in the progression of IgA nephropathy remains unresolved. We evaluated two genetic polymorphisms (rs11552708 and rs3803800) and plasma levels of TNFSF13 in 637 patients with IgA nephropathy, and determined the risk of ESRD according to theses variable. Neither of the examined genetic polymorphisms associated with a clinical outcome of IgA nephropathy. However, high plasma levels of TNFSF13 increased the risk of ESRD. To explore the causal relationship and underlying mechanism, we treated B cells from patients ( n =21) with or without recombinant human TNFSF13 (rhTNFSF13) and measured the expression of IgA and galactose-deficient IgA (GdIgA) using ELISA and flow cytometry. Treatment with rhTNFSF13 significantly increased the total IgA level among B cells, and TNFSF13 receptor blockade abrogated this increase. Furthermore, the absolute levels of GdIgA increased with rhTNFSF13 treatment, but the total IgA-normalized levels did not change. Both RNA sequencing and quantitative PCR results showed that rhTNFSF13 did not alter the expression of glycosyltransferase enzymes. These results suggest that high plasma TNFSF13 levels associate with a worse prognosis of IgA nephropathy through the relative increase in GdIgA levels.
A6.21 NF-κB-inducing kinase (NIK) is expressed in synovial endothelial cells in early arthritis patients and correlates with markers of inflammation
Authors: KI Maijer, AR Noort, MJH de Hair, C van der Leij, KPM van Zoest, IY Choi, DM Gerlag, M Maas, PP Tak, SW Tas
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background and objectives</h3> The NF-κB family of transcription factors is strongly involved in synovial inflammation. We have previously shown that NF-κB-inducing kinase (NIK) is a key regulator of inflammation-induced angiogenesis in rheumatoid arthritis (RA) synovial tissue (ST). Here, we investigated synovial NIK expression in early arthritis patients and in autoantibody-positive individuals at risk for developing RA. <h3>Materials and methods</h3> ST biopsies were obtained via arthroscopy from 154 early arthritis patients (arthritis duration &lt;1 year) with different diagnoses and from 54 IgM-rheumatoid factor and/or anti-citrullinated peptide antibody-positive individuals at risk for developing RA, without any evidence of arthritis. ST was stained for NIK and the endothelial cell (EC) marker vWF. Additionally, measures of disease activity (ESR, CRP, joint swelling) were collected and contrast-enhanced MRI was performed in a subset of these patients. <h3>Results</h3> In early arthritis patients, NIK was predominantly expressed in EC of small blood vessels. Furthermore, NIK expression correlated with ESR (r = 0.184; p = 0.024), CRP (r = 0.194; p = 0.017), joint swelling (r = 0.297; p &lt; 0.001), synovial cellular markers (CD68<sup>+</sup> lining and sublining macrophages r = 0.585; p &lt; 0.001 and r = 0.728; p &lt; 0.001, respectively, CD3<sup>+</sup> T cells r = 0.733; p &lt; 0.001 and CD22<sup>+</sup> B cells r = 0.264; p = 0.040), MRI effusion (r = 0.665; p &lt; 0.001), MRI synovitis (r = 0.632; p &lt; 0.001) and MRI total score (r = 0.569; p &lt; 0.001). In 18.5% of autoantibody-positive individuals ST NIK<sup>+</sup>EC were present, but this was not predictive for the development of arthritis. <h3>Conclusions</h3> NIK<sup>+</sup>EC are present in the earliest phase of synovial inflammation and may be indicative of high angiogenic activity in the inflamed ST. Therefore, NIK<sup>+</sup>EC may play an important role in the persistence of synovitis. Collectively, our data underscore the importance of angiogenesis in synovial inflammation and identify NIK as a potential therapeutic target in arthritis.
Intraoropharyngeal U-turn method using transnasal esophagogastroduodenoscopy
Authors: Kenro Kawada, Takuya Okada, Taro Sugimoto, Seiji Kishimoto, Tatsuyuki Kawano
Journal: Endoscopy (2014)

The more progress that has been made in endoscopy, the more superficial cancers have been found in the head and neck regions [1] [2] [3] [4]. There are, however, some areas that are difficult to observe on transoral esophagogastroduodenoscopy (EGD). In particular, only a tangential view of the radix linguae is obtained with transoral EGD. In this report, we present the procedure for an intraoropharyngeal U-turn method using transnasal EGD [5], which we used to diagnose a superficial squamous cell carcinoma of the radix linguae.
PTH-126 A Functional Model of of a ‘Seven Day Acute Gastroenterology Service’: Looking Beyond Out-Of-Hours Endoscopy
Authors: A Jawhari, L Meran, A McCarthy, N Lewis, G Aithal
Journal: Gut (2013)

<h3>Introduction</h3> Early involvement and management by specialists has been shown to have a favourable impact on outcomes in a number of acute medical conditions. Increased patient mortality at weekends has also been attributed to limited access to specialist services. While an increasing number of hospitals provide an out-of-hours service for upper gastrointestinal bleeding, examples of a comprehensive acute Gastroenterology services are infrequent. <h3>Methods</h3> In January 2007, we established an acute gastroenterology service to provide consultant-lead assessment and management for all patients identified through acute servieces with symptoms related to gastrointestinal and hepato-pancreatico-biliary conditions. The consultant of the week lead and delivered the service supported by a registrar and a dedicated inpatient endoscopy team, free from any commitment to elective services. Inpatient care was supported by daily consultant led ward rounds. We achieved the ‘critical mass’ to deliver this service by consolidating all inpatient work on on site. <h3>Results</h3> Mean LOS of all patients discharged with a gastroenterology HRG, from the specialist gastroenterology ward was 7.6 days, and from non-specialist medical beds was 9.6 days. Overall the specialist gastroenterology ward provided care for 1.8 times more patients compared with other Medical wards of the same size at NUH. Actual mean LOS (for the period of 2011–2012) was significantly shorter than the ‘expected’ LOS, and than mean peer LOS for 4 main diagnostic categories (table) with no significant increase in readmission rates. Discharge rates were maintained at the same level during the weekend (mean 4 discharges per weekday and on Saturday, with a peak of 6 discharges on Fridays and a dip to a mean of 3 discharges on Sunday. <h3>Conclusion</h3> Specialist led care can be provided to all patients with acute gastrointestinal and hepato-pancreatico-biliary conditions. A functional 7-day ‘acute gastroenterology’ can be sustained to provide high quality and intensity of care with favourable outcomes. <h3>Disclosure of Interest</h3> None Declared.