tbuckley/cabot-search huggingface dataset:
Treatment of Vasospastic Amaurosis Fugax with Calcium-Channel Blockers
Authors: Jacqueline Winterkorn, Mark J. Kupersmith, Jonathan D. Wirtschafter, Scott Forman
Journal: New England Journal of Medicine (1993)

Amaurosis fugax, a brief loss of vision in one eye, is caused by hypoperfusion of the retinal circulation. If an attack of amaurosis is prolonged, the patient is at risk of permanent visual loss. Since the 1950s, amaurosis fugax has usually been attributed to embolism from the heart or great vessels1 or to carotid occlusive disease. Because of the difficulty in differentiating between these and other causes, amaurosis fugax remains a therapeutic challenge. Depending on the patency of the internal carotid arteries, the principal treatments are anticoagulation or antiplatelet therapy2 and carotid endarterectomy3. Vasospasm is rarely reported as a . . .
436P Efficacy and safety of necitumumab (neci) in East Asian (EA) patients (pts) with stage IV squamous non-small-cell lung cancer (NSCLC): a subanalysis of the SQUIRE trial
Authors: K. Park, E.K. Cho, M. Bello, M.-J. Ahn, S. Thongprasert, E.-K. Song, V. Soldatenkova, H. Depenbrock, T. Puri, M. Orlando
Journal: Annals of Oncology (2015)
2′-O-methyl-modified RNAs Act as TLR7 Antagonists
Authors: Marjorie Robbins, Adam Judge, Lisa Liang, Kevin McClintock, Ed Yaworski, Ian MacLachlan
Journal: Molecular Therapy (2007)

RNA molecules such as single-stranded RNA (ssRNA) and small interfering RNA (siRNA) duplexes induce Toll-like receptor (TLR)-mediated immune stimulation after intracellular delivery. We have previously shown that selective incorporation of 2'-O-methyl (2'OMe) residues into siRNA abrogates cytokine production without reduction of gene silencing activity. Here we show that 2'OMe-modified RNA acts as a potent inhibitor of RNA-mediated cytokine induction in both human and murine systems. This activity does not require the direct incorporation of 2'OMe nucleotides into the immunostimulatory RNA or that the 2'OMe nucleotide-containing RNA be annealed as a complementary strand to form a duplex. Our results indicate that 2'OMe RNA acts as a potent antagonist of immunostimulatory RNA. We further show that 2'OMe RNA is able significantly to reduce both interferon-alpha (IFN-alpha) and interleukin-6 (IL-6) induction by the small-molecule TLR7 agonist loxoribine in human peripheral blood mononuclear cells (human PBMCs), in murine Flt3L dendritic cells (Flt3L DCs), and in vivo in mice. These results indicate that 2'OMe-modified RNA may have utility as an inhibitor of TLR7 with potential applications in the treatment of inflammatory and autoimmune diseases that involve TLR7-mediated immune stimulation.
GROUP PSYCHOTHERAPY
Authors: SAMUEL B. HADDEN
Journal: American Journal of Psychiatry (1944)

Four years experience with the group method indicates that it is one which may solve the problem of giving adequate psychiatric care to greater numbers of neurotic patients. All of the psychotherapeutic methods of individual therapy are utilized, and their effect seems to be increased in the group because contact with other sufferers gives the patient courage and causes him to lose his feelings of isolation. He is inspired by his fellow sufferers to greater effort in his own behalf. As he improves and can offer encouragement to newcomers he gains a sense of being important in the lives of others. These mechanisms seem to give group treatment greater force than individual therapy. The method permits students to observe and participate in a dynamic psychotherapeutic relationship and has been shown to be of value in military service. The method will establish psychotherapy as a practical procedure just as the reduction in cost of manufacture of insulin and sulfanilamide has made them readily available.
Harm reduction interventions should encompass people who inject image and performance enhancing drugs
Authors: Jim McVeigh, Andreas Kimergård, Geoff Bates, Vivian D Hope, Fortune Ncube
Journal: BMJ (2016)

We welcome the news item on the role of harm reduction in the “fight” against HIV,1 and we broadly agree with the findings of the report The Case for a Harm Reduction Decade: Progress, Potential and Paradigm Shifts .2 Clearly harm reduction for people who inject drugs is having a positive impact on HIV in many places around …
Abstract 1740: Over-expression of gelsolin protein in human cervical carcinoma associate with cell migration in vitro and recurrence-free survival in vivo
Authors: Tzu-I Wu, Chia-Jung Liao, Ting-Chang Chang, Kwang Huei Lin
Journal: Cancer Research (2010)

Abstract Introduction: The global yearly incidence and mortality of cervical cancer are 471,000 annual new cases and 233,000 deaths. Almost 80% cases occur in less developed countries, where it accounts for 15% of cancer in women. Cervical carcinoma ranks as the leading malignancy of genital tract malignancy in the developing countries. The recurrence indicate poor prognosis while the clinicopathological risk factors exist. In order to identify the potential molecular biomarker, proteomic analysis was performed and gesolin protein was selected. Materials and Methods: The plasma from normal healthy and cervical cancer, and tissue of cervical cancer plus adjacent normal tissue were collected for Western blotting assay, enzyme-linked immunosorbent assay (ELISA) or immunohistochemical stain (IHC). One hundred and twenty four patients with cervical cancers underwent surgical management were enrolled to evaluate IHC Histoscore of gesolin according to the multiply of immunoreactivity and involved percentage. The Transwell assay was conducted for cell migration analysis in HeLa cell line. The statistical analysis include independent t tests, Kaplan-Meier method using the log-rank test and the Cox proportional hazard model Receiver operating characteristics (ROC) curve analysis was used to determine cut-off points of the predictive parameters in univariate analysis. Results: The Western blot and ELISA assay both demonstrated the overexpression of plasma gelsolin in cases with cervical cancer rather than normal population, and with staged III/IV rather than stage I/II cases, In addition, gelsolin protein level also increased in conditional medium of cultured HeLa cell. Thus in vitro cell migration assay revealed decreased cell numbers while adding the gelsolin neutralizing antibody or small interference RNA compared with adding mock, non-specific antibody or Luciferase-shRNA. The other clinical parameters including stage, tumor size and lymph node status also put into consideration. The best cut-off point of gesolin Histosore to clarify the recurrence by ROC curve analysis is 115. The gelsolin Histosore (≤ 115 vs &amp;gt; 115, P= 0.018) and lymph node metastasis (no vs yes, P&amp;lt;0.001) play important prognostic role in recurrence by multivariate analysis. Conclusion: Overexpression of plasma and cytoplasmic gelsolin protein in cervical cancer promote cell migration by binding to actin cytoskeleton for rearrangement and cell motility. Among the clinicopathological parameters, the gelsolin overexpression and lymph node metastasis disclosed the recurrence-free survival impact by multivariate analysis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1740.
Endoscopic ultrasound-guided placement of fiducial markers using a novel “wet-fill technique” without a bone wax seal
Authors: Harshit Khara, John Pineda-Bonilla, Kimberly Chaput, Amitpal Johal
Journal: Endoscopy (2013)

Endoscopic ultrasound (EUS)-guided placement of fiducial markers for image-guided radiation therapy of gastrointestinal malignancies is usually accomplished by backloading a 22-gauge EUS-fine needle aspiration (FNA) needle with a thinner gold fiducial marker and sealing the needle tip with bone wax [1] [2] [3]. We describe a novel "wet-fill technique" for loading a fiducial marker into a needle and placing it in a tumor under EUS guidance that avoids the use of bone wax. The new technique has been used in one patient with pancreatic cancer and three patients with esophageal cancer.
Abstract 2085: MMP-2 knockdown leads to anoikis and inhibits migration and invasion by attenuating αvβ3/EGFR/Pak4 survival signaling in human glioma xenograft cells <i>in vitro</i> and <i>in vivo</i>
Authors: Divya Kesanakurti, Chandramu Chetty, Dilip Maddirela, Dzung H. Dinh, Jasti S. Rao
Journal: Cancer Research (2012)

Abstract MMP-2 plays a key role in the degradation of the extracellular matrix and thereby enhances the proliferative, invasive and metastatic potential of cancer cells. MMP-2 knockdown using siRNA (pM) in human glioma xenograft cell lines, 4910 and 5310, sensitized cells to anoikis associated with cleavage of PARP, and caspases 8 and 3. pM-inhibited cell adhesion, invasion and migration correlated with attenuation of EGFR, c-Src and Pak4 phosphorylation. High expression of Pak4 in human glioma cell lines and tumor tissues implicated its essential role in increasing glioma malignancy. Co-immunoprecipitation studies implied that MMP-2 directly interacts by complex formation with EGFR, p130CAS and Pak4 along with αvβ3 integrin and pM treatment disrupted this direct binding. Vitronectin-mediated αvβ3 activation and EGF and rhMMP-2 supplementation counteracted pM-inhibited, Pak4-mediated migration and invasion. Conversely, αvβ3 blocking antibody and ErbB-2/EGFR inhibitor (GW2974) further reduced pM-inhibited, Pak4-mediated invasiveness and migration, thereby suggesting a role of αvβ3 and EGFR in the regulation of Pak4 survival signaling. Individual treatments of pM and Pak4.si led to considerable anoikis in both 4910 and 5310 cells and simultaneous co-depletion MMP-2 and Pak4 resulted in robust increase in anoikis levels. In vivo orthotopic experiments in nude mice revealed decreased tumor size with pM treatment. Immunofluorescence analysis of pSV-tumor sections showed high expression and co-localization of MMP-2/αvβ3, which decreased upon treatment with pM along with significant decrease in phospho-EGFR, phospho-Pak4, Bcl2 and Bcl-xL levels. Our results imply a possible crosstalk between MMP-2 and Pak4 and suggest the potential of targeting both MMP-2 and Pak4 as a future therapeutic approach in glioma therapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2085. doi:1538-7445.AM2012-2085
Case 16-2013
Authors: Suzanne L. Bender, Nicole A. Sherry, Ricard Masia
Journal: New England Journal of Medicine (2013)

A 12-year-old girl with a history of celiac disease, anxiety, and depression was seen in the outpatient psychiatry clinic of this hospital because of irritability, hypersomnia, and multiple somatic symptoms. Psychotherapy and the administration of antidepressants were begun.
Clinical Evaluation of the DNA Bentonite Flocculation Test for Systemic Lupus Erythematosus
Authors: Donald E. Kayhoe, John P. Nasou, John Bozicevich
Journal: New England Journal of Medicine (1960)

WE recently described the adaptation of the bentonite flocculation technic1 to the demonstration of the reaction of lupus serum with highly polymerized desoxyribonucleic acid (DNA). The report that follows is a clinical evaluation of this new test, emphasizing studies that indicate its frequent reactivity with serum in patients with active systemic lupus erythematosus and its nonreactivity with the serum of patients with rheumatoid arthritis and a variety of other diseases.Materials and MethodsIn the study one or more serum specimens were obtained from 13 patients with active systemic lupus erythematosus, 12 patients with systemic lupus in remission, 8 patients . . .
Purine Nucleoside Phosphorylase Deficiency Associated with Selective Cellular Immunodeficiency
Authors: J. W. Stoop, B. J. M. Zegers, G. F. M. Hendrickx, L. H. Siegenbeek van Heukelom, G. E. J. Staal, P. K. de Bree, S. K. Wadman, R. E. Ballieux
Journal: New England Journal of Medicine (1977)

We studied a 15-month-old girl who had normal T-cell and B-cell immunity at birth, after which a gradual decrease in T-cell immunity developed. This selective cellular immunodeficiency was inherited as an autosomal recessive trait: two older sisters had the same immunodeficiency. Adenosine deaminase activity was present in erythrocytes and lymphocytes of the patient, parents and a healthy brother. Purine nucleoside phosphorylase activity was not found in the patient's erythrocytes and lymphocytes (the parents and brother had intermediate values, indicating that the enzyme deficiency too was inherited as an autosomal recessive trait). Analysis of serum and urine from the patient and of serum from her two deceased sisters showed high levels of inosine and guanosine in addition to hypouricemia and hypouricosuria. The bone marrow was megaloblastic, and the blood hypochromic microcytic. The patient had spastic tetraparesis. Intoxication of the T lymphocytes after birth by metabolic products may explain the progressive cellular immunodeficiency.
Abstract 5295: S100A4 regulates tumor growth, motility, and invasion in pancreatic cancer
Authors: Hitoshi Sekine, Takahiro Tabata, Nobukazu Tsukamoto, Akihiro Yamamura, Keiko Abe, Yuki Yoshino, Keisuke Sato, Na Chen, Yukiko Umetsu, Daisuke Sato, Zhaodi Gu, Shinichi Fukushige, Kentaro Shima, Fuyuhiko Motoi, Shinichi Egawa, Makoto Sunamura, Akira Horii
Journal: Cancer Research (2010)

Abstract S100A4 protein belongs to the S100 subfamily, which has grown to be one of the large subfamilies of the EF-hand Ca2+-binding proteins, and overexpression of S100A4 is suggested to associate with cell proliferation, invasion, and metastasis. We observed frequent overexpression of S100A4 in pancreatic cancer cell lines by quantitative real-time RT-PCR and Western blotting. Tissue microarray analysis validated S100A4 overexpression in primary pancreatic cancer tissues, and overexpression of S100A4 associated with perineural invasion. We further analyzed RNAi-mediated knockdown to address the possibility of its use as a therapeutic target for pancreatic cancer. The specific knockdown of S100A4 strongly suppressed cell growth, induced G2 arrest and eventual apoptosis, and decreased cell migration. Furthermore, microarray analyses revealed that knockdown of S100A4 induced expression of the tumor suppressor genes PRDM2 and VASH1. We further established stably S100A4-expressing cell lines using two pancreatic cancer cell lines with low level of S100A4 expression; remarkable accelerated cell growth and motility was observed after induction of S100A4, whereas no change was observed in the control cells. Comparison of the expressional profiles after siRNA-mediated knockdown and expression vector-mediated induction is underway, and the results will also be demonstrated and discussed. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5295.
OP0074 First Report of Circulating Micrornas in Tumour Necrosis Factor Receptor-Associated Periodic Syndrome (TRAPS)
Authors: M. Galeazzi, L. Obici, M. Ferracin, V. Fulci, M. F. McDermott, O. M. Lucherini, G. Merlini, L. Dickie, I. Muscari, B. Frediani, M. Negrini, R. Cimaz, L. Cantarini
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Tumor necrosis factor-receptor associated periodic syndrome (TRAPS) is the most common autosomal dominant autoinflammatory disorder. To the best of our knowledge circulating miRNAs in TRAPS, as well as in other monogenic autoinflammatory disorders have never been investigated. <h3>Objectives</h3> to evaluate circulating microRNAs (miRNAs) levels in patients with TRAPS, in comparison to healthy controls, and to correlate their levels to parameters of disease activity and/or disease severity <h3>Methods</h3> expression levels of circulating miRNAs were measured by Agilent microarrays in 29 serum samples from 15 TRAPS patients carrying mutations known to be associated with high disease penetrance and 8 healthy controls. Differentially expressed and clinically relevant miRNAs were detected using GeneSpring GX software <h3>Results</h3> we identified a 6 miRNAs signature able to discriminate TRAPS from healthy controls. Moreover, 4 miRNAs were differentially expressed between patients treated with the interleukyn (IL)-1 receptor antagonist anakinra and untreated patients. Of them, miR-92a-3p expression was found to be reduced in untreated patients, while its expression levels were similar to healthy controls in those samples from patients during anakinra treatment. MiR-92b levels were inversely correlated with the number of fever attacks/year during the 1<sup>st</sup> year from the index attack of TRAPS, while miR-377-5p levels were positively correlated with serum amyloid A (SAA) circulating levels. <h3>Conclusions</h3> serum miRNAs levels show a baseline pattern in TRAPS, and may serve as potential markers of response to therapeutic intervention <h3>Disclosure of Interest</h3> None Declared
SAT0292 Clinical, Imaging and Laboratory Variables Fail to PREDICT Morphological Vascular Progression in Takayasu Arteritis
Authors: S. Sartorelli, M.C. Di Chio, E.M. Baldissera, M. Papa, F. De Cobelli, M.G. Sabbadini, A.A. Manfredi, E. Tombetti
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Takayasu arteritis (TA) is an orphan idiopathic inflammatory disease. A major pitfall in TA is the absence of a reliable modality to describe disease evolution and activity. Morphological vascular progression represents a significant clinical outcome, given the prognostic impact of cardiovascular complications. <h3>Objectives</h3> We verified if variables currently used in clinical practice as well as functional imaging data could predict the concurrent morphological progression. <h3>Methods</h3> We used Magnetic Resonance Angiography (MRA) as the reference for the assessment of disease evolution and morphological progression and we performed an exhaustive follow-up of patients with multiple MRA assessments. We selected and retrospectively evaluated 16 TA patients, for which 38 couples of MRA examinations performed within 24 months were available. Morphological progression within each couple was defined as the occurrence of new lesion(s) or worsening of at least one of the pre-existing lesions. Wall contrast enhancement (CE) was expressed as the difference of CNR (Contrast to Noise Ratio) pre- and post- infusion of contrast medium. <h3>Results</h3> Morphological progression occurred in 18 out of 38 couples of MRA examinations, despite effective treatment selected based on the most recent information available in the literature. All variables commonly used in the clinical practice failed to predict TA progression, except for maximum neutrophils values and radiological progression in the year preceding the first MRA of each couple. Wall CE at the first MRA examination within each couple was not associated with progression at the following MRA examination. <h3>Conclusions</h3> Most clinical, laboratory and functional imaging variables, currently associated with TA activity, fail to predict the vascular progression. Since they well reflect the activation of cardinal systemic inflammatory pathways, our data suggest that other inflammatory pathways contribute to the clinical outcomes of TA patients. Their identification and as such the identification of more effective targeted molecular therapies represent a major unmet medical need. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5598
Mutations in the Factor XII Gene in Solitary Cases of Recurrent Angioedema with Normal C1 Inhibitor Induced or Worsened by Oral Contraceptives or Hormonal Replacement Therapy
Authors: C. Stanger, K. Wulff, J. Hardt, G. Witzke, K. Bork
Journal: Journal of Allergy and Clinical Immunology (2012)
Case 6-2016
Authors: Jeffrey A. Biller, S. Reha Butros, Gayun Chan-Smutko, Annah N. Abrams, Daniel C. Chung, Catherine E. Hagen
Journal: New England Journal of Medicine (2016)

A 10-year-old boy was seen in the gastroenterology clinic because of abdominal cramping and fevers. Abdominal imaging studies revealed circumferential thickening of a segment of the colonic wall and mesenteric lymphadenopathy. A diagnostic procedure was performed.
FRI0080 Bone Mineral Density, Sclerostin and Insulin Are Independently Associated with Coronary-Artery Atherosclerosis in Patients with Established Rheumatoid Arthritis
Authors: M. Bernardes, A.J. Madureira, M.J. Martins, T. Vieira, D. Gonçalves, J.G. Pereira, J.C. Machado, L. Costa, I. Ramos, F. Simões-Ventura, E. Martins
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Rheumatoid arthritis (RA) is associated with premature atherosclerosis and increased prevalence of cardiovascular disease (CVD). Inverse associations have been described between bone mineral density (BMD) and Coronary Artery Calcification (CAC) in different diseases. <h3>Objectives</h3> To determine the degree of association of CAC Score (CACS) with bone density, bone markers and CVD risk factors in patients with established RA without known coronary disease, nor CV events. <h3>Methods</h3> HAQ and DAS28 (4v) were obtained. ESR, CRP, traditional CVD risk factors, homocysteine, insulin, BNP, serum β-CTX1, osteocalcin, Dkk-1, sclerostin, RANKL and OPG were determined. BMD was assessed by DeXA (Lunar Expert® 1320) at the hip, lumbar spine and hands. CACS was evaluated using a 64-slice computed tomography scanner, Somatom Sensation Cardiac 64 (Siemens, Germany). Coronary calcium was measured by Argus software; CACS was determined by the Agatston score. A multivariate analysis model was used for statistical analysis (IBM SPSS Statistics 21). <h3>Results</h3> We evaluated 57 RA patients, 42 (74%) women, age 54±12 years, disease duration 14±10 years, mean DAS28 (4v) of 4.15±1.24 and a mean HAQ of 1.32±0.69. In our sample, 29 (51%) were under biologics. CAC was detected in 31 (54%) patients (CACS ≤10: 37 (66%); 10&lt; CACS ≤100: 10 (17%); CACS &gt;100: 10 (17%). Lumbar spine, total hip, femoral neck, hands and second proximal phalanges BMD are inversely associated with CACS (p&lt;0.05) after adjusting for age, disease duration, body mass index, DAS28 (4v), current HAQ, time under DMARDs therapy, years of corticosteroid use and years of bisphosphonates use. In terms of bone metabolism, lower levels of sclerostin were associated with higher CACS (p&lt;0.05) (after adjusting for age, age at diagnosis, disease duration, body mass index, DAS28 (4v), current HAQ, current average daily dose of prednisone and years of bisphosphonates use). None of the traditional CVD risk parameters were associated with CACS. Insulinemia was inversely associated with CACS (p&lt;0.001), after adjusting for age, age at diagnosis, disease duration, body mass index, DAS28 (4v), current HAQ, time under DMARDs therapy, time under biologics, years of corticosteroid use and current average daily dose of prednisone. <h3>Conclusions</h3> Our RA population reproduced the inverse relationship between, either systemic or localized, BMD and CAC, supporting the hypothesis that bone metabolism and atherosclerotic plaque mineralization are related processes. As in the african american-diabetics, sclerostin was negatively associated with CACS in our RA patients. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3921