tbuckley/cabot-search huggingface dataset:
Abstract 1985: The activation of GSK3 by HGF-MET axis is responsible for differentiation of rhabdomyosarcoma cells
Authors: Katarzyna Miekus, Wojciech Bobela, Marcin Majka
Journal: Cancer Research (2011)

Abstract Introduction: Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma among children. At the late stages (metastatic) the outcome for the patients is very poor. The molecular basis for loss of cell differentiation in the process of carcinogenesis is not well understood. Differentiation status of rhabdomyosarcoma cells strongly influences its progression and metastatic ability and therefore the overall patient survival. At the onset of myogenic differentiation MET receptor is rapidly downregulated and on the other hand the activation of MET receptor in rhabdomyosarcoma has been shown to influence proliferation, survival and migration. Aim: Dissecting the role of HGF-MET receptor axis and GSK3 activation in the process of RMS differentiation. Materials and methods: Cell lines used in experiments: RH30 (ARMS), SMS-CTR (ERMS). Real time RT-PCR and western blotting were used to evaluate gene expression and the activation of various intracellular signaling pathways, respectively. The evaluation of protein activation in RMS cell lines was performed after stimulation with HGF. Results: We found that, during differentiation process the expression of MyoD, characteristic for poorly differentiated rhabdomyosarcoma cells decreased and the expression of myogenin, protein characteristic for differentiated/ matured muscle cells increased. That was paralleled by decreased expression of MET receptor. In RMS cell lines with stable downregulation of MET receptor the expression of differentiation related genes was similar to differentiated RMS cells. When we study the activation of GSK3 we observed the phosphorylation of GSK3 beta on serine 9 after HGF stimulation. The intracellular pathway responsible for GSK3 beta phosphorylation turn out to be PI3K-AKT dependent. We observed also the accumulation of beta-catenin in the nucleus of RMS cells stimulated with HGF. This effect was father augmented when HGF was used together with BIO, small molecular inhibitor of GSK3 beta. Conclusion and future directions: In this study we have shown that the expression and signaling of MET receptor is responsible for differentiation of RMS cells. Moreover we postulate that MET receptor exerts its influence on RMS cells differentiation through GSK3 beta activation. These findings might have the significant clinical implication for the treatment of RMS cells because they suggest that induction of differentiation of RMS cells by blocking MET receptor could inhibit growth of RMS tumors. Finally, we think that also GSK3 beta could be used as the new therapeutic target to induce differentiation of RMS. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1985. doi:10.1158/1538-7445.AM2011-1985
Noninvasive Evaluation of Cardiac Function in Hypothyroidism
Authors: William F. Crowley, E. Chester Ridgway, Edward W. Bough, Gary S. Francis, Gilbert H. Daniels, Ione A. Kourides, Gordon S. Myers, Farahe Maloof
Journal: New England Journal of Medicine (1977)

Left ventricular performance was studied in 15 patients with severe, primary hypothyroidism (mean serum total thyroxine of 0.8 mug per 100 ml and serum thyrotropin of 160 muU per milliliter). Pretreatment systolic-time intervals were characterized by prolongation of the pre-ejection period (delta PEP = +30) and reduction of the left ventricular ejection period (delta LVET = -23) with a resultant increase in the PEP/LVET ratio (0.47). Nine of 14 patients demonstrated pericardial effusions. These abnormalities were reversed with physiologic thyroxine replacement. Further reductions of the delta PEP and PEP/LVET ratio occurred with supraphysiologic doses (200 to 300 mug per day). During therapy, delta PEP was inversely correlated with serum thyroxine (P less than 0.001) and directly correlated with serum thyrotropin (P less than 0.001). Thus physiologic thyroid hormone replacement, appropriately adjusted to need, appears necessary in hypothyroidism for optimal left ventricular function.
AB0727 Bone Mineral Density Depending on the Activity of Ankylosing Spondylitis in Men
Authors: O. Pirogova, T. Raskina, O. Malyeshenko, V. Fanaskov
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> infringement of a metabolism of bone tissue caused by inflammation, increases the risk of developing osteoporosis (OP) and can be considered as an indicator of "seriousness" of the disease and the activity of inflammation. <h3>Objectives</h3> assess bone mineral density in men with ankylosing spondylitis (AS) depending on x-ray stage of the disease. <h3>Methods</h3> Under the supervision were 70 male patients with a diagnosis of as. The average age of patients 43,2±9.1 years. Patients were included in the study in a different time period from the onset of the speakers: 4.3 per cent in the first year of illness, 21,4% - while ago as 1 - 4 years, with the duration of 5-9 years - 25,7% and 48.6% of patients have 10 years or more. Among the 70 patients of 62.8% had only axial defeat. In 60,0% of cases in the radiography of spine identified Sindesmofitis, of them in the lumbar spine of 52.3% of patients, breast - 45.2% and in the cervical - 35,7%. Sindesmofitis in two departments of a backbone identified the 35,7%, and in all departments, 15% of patients, till the formation of the symptoms of "bamboo sticks." <h3>Results</h3> When comparing the densitometric parameters in the groups of patients depending on radiological stage statistically significant decreasing of densitometric parameters of the proximal femur as the progression of radiographic changes. So, in men with I-II x-ray stage AU IPC hip was 947,8±15,13 g/cm<sup>2</sup>, which corresponds to -2,16±0,12 standard deviations by Z-test, while in stage III-IV AC IPC - 880,9±26,83 g/cm<sup>2</sup>, which corresponds to -2,66±0,19 standard deviations on the Z-test (p=0,0136 and R?0,001). However, in the lumbar spine densitometric parameters IPC increase as the progression of radiological stage. IPC at I-II stage was 1007,33±27,76 g/cm<sup>2</sup> and -1,08±0.15 standard deviations by Z-test compared with the IPC with stage III-IV AC 1179,81±31,94 g/cm<sup>2</sup> and -0,20±0,17 standard deviations on the Z-test (p=0,0106 and p=0,0216). <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4162
FOCUSED, ABBREVIATED TRAINING IN THE PERFORMANCE AND INTERPRETATION OF TRANSTHORACIC ECHOCARDIOGRAPHY SHOWS PROMISE IN RURAL UGANDA
Authors: Catherine Bennet, Fardous Charles Abeya, Ari Hoffman, Joselyn Rwebembera, Malissa Wood, Michael Picard, Samson Okello
Journal: Journal of the American College of Cardiology (2016)
PD02-07: Models Predicting Non-Sentinel Node Involvement in Breast Cancer Also Predict for Regional Recurrence If the Axilla Is Not Treated.
Authors: M Pepels, H Vestjens, M de Boer, P Bult, J van Dijck, M Mencke, P van Diest, G Borm, V Tjan
Journal: Cancer Research (2011)

Abstract Background Series on breast cancer patients with sentinel node (SN) isolated tumor cells or micrometastases show low recurrence rates in the presence of SN isolated tumor cells, and varying recurrence rates in the presence of SN micrometastases. Non-SN prediction models are frequently used as a decision aid to identify patients that may not need axillary treatment, but this still needs to be validated in respect to regional recurrence. Patients and Methods We followed a cohort of 486 patients in the MIRROR-study of whom none received axillary treatment with favorable primary tumor characteristics and pN0(i+)(sn) or pN1(mi)(sn) for a median of 5 years. The patients were categorized into low or high-risk subgroups based on several published non-SN prediction models (cut-off levels between models varied from 3 to 10%), i.e. the Tenon Scoring system, MSKCC nomogram, Stanford nomogram and a Dutch model (Bolster et al.). The primary endpoint was 5-year regional recurrence-free survival. Results The overall 5-year regional recurrence rate was 3.0% (+/− 0.1%). Using the Tenon scoring system, we identified 438 patients with a low risk score of 3.5 or lower with a 5-year regional recurrence rate of 2.3% (+/− 0.8%), compared to a recurrence rate of 10.1% (+/− 0.4%) in 48 patients with a score above 3.5. For the MSKCC nomogram a low risk score of 0.10 or lower identified 300 patients with a 5-year recurrence rate of 2.8% (+/− 1.1%), and a score above 0.10 identified 166 patients with a recurrence rate of 3.4% (+/− 1.5%) (20 patients not assessable). By the Stanford nomogram a low risk cutoff level of 10% identified 21 patients without recurrence, whereas 465 patients had a 3.2% (+/−0.9%) recurrence rate. Using a Dutch model, a low risk cutoff score of 20 discriminated between 384 patients with a 5-year recurrence rate of 2.2% (+/− 0.8%) and 102 patients with a recurrence rate of 6.3% (+/− 2.9%). Further analyses with different cut-off values and subgroups will be presented at the conference. Conclusion Using several published non-SN prediction models for predicting regional recurrence, the Tenon scoring system outperformed the other models in that it identified the largest subgroup of patients with a low recurrence rate. We would recommend axillary treatment in patients classified as high risk according to the Tenon score. Funding: Netherlands Organization for Health Research and Development (ZonMw 945-06-509). Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr PD02-07.
Experimental approaches for exposure to sized glass fibers.
Authors: D M Bernstein, R T Drew, M Kuschner
Journal: Environmental Health Perspectives (1980)

A number of studies have shown that glass fibers induce both malignant mesothelioma and fibrosis in rats and that these reactions may be primarily a function of the physical properties of the fiber. However, these studies were carried out with fibers having broad size distributions and used methods of administration which bear little resemblance to the way man is exposed. To better characterize the health effects of glass fibers, techniques have been developed to expose rats to glass fibers of defined sizes by intratracheal instillation of aqueous suspensions and by
FIRST PASS CARDIAC IMAGING USING A SINGLE CRYSTAL GAMMA CAMERA/COMPUTER SYSTEM
Authors: M. W. Groch, E. L. Lands, H. J. Palay, D. Willems, S. M. Spies, P. J. Groch, T. J. Freeman
Journal: Clinical Nuclear Medicine (1997)

Scientific Abstracts Central Chapter, Society Of Nuclear Medicine Meeting, April 11-13, 1997, Grand Geneva Resort, Lake Geneva, Wi
Abstract 2649: Human adipose tissue-derived mesenchymal stem cells can target brain tumor initiating cells.
Authors: Seung Ah Choi, Kyu-Chang Wang, Ji Hoon Phi, Ji Yeoun Lee, Jung Won Choi, Hyung Ah Kim, Se Hee Kim, Yong Hwy Kim, Sung Eun Kwon, Young-Hoon Kim, Seung-Ki Kim
Journal: Cancer Research (2013)

Abstract Abstract The discovery of neural stem cells offers paradigm shift in brain tumor research. In neuro-oncology, the biology of neural stem cells has been pursued in two ways: as cancer stem cells to understand the origin and maintenance of brain tumors and as a potential cell-based vehicle for gene therapy. In addition to neural stem cells, mesenchymal stem cells (MSCs) have been reported to possess tumor-tropic migratory capacities. However, there is scanty data on migratory capacity of MSC toward brain tumor initiating cells (BTICs). This study focuses on investigating the ability of human adipose tissue derived MSCs (hAT-MSCs) to target BTICs and their cross-talk in the microenvironment. BTICs were isolated from three different kinds of brain tumors (medulloblastoma, atypical teratoid/rhabdoid tumor (ATRT), glioblastoma). The migration capacities of hAT-MSCs toward BTICs were examined both in vitro transwell assay and in vivo bioluminescence imaging analysis. To investigate the crosstalk between hAT-MSCs and BTICs, various cytokines were analyzed. Using co-culture system of hAT-MSCs and BTICs, we analyzed the mRNA expression patterns of cytokine receptors (CCR2, CCR4, CCR5, CCR7, CCR9, CCR10, XCR1, CXCR1, IL-8R, CXCR4, CX3CR1, IL1R1, IL6R, MET, PDGFRB, KDR, CD44, IFNAR1 and TEK) by qRT-PCR and protein level of their ligand in co-cultured media by Bio-Plex human cytokine ELISA. We confirmed the migratory capacity of t hAT-MSCs toward BTICs in vitro and BTICs-derived xenograft brain tumors in vivo live imaging. mRNA expression of receptors (CCR4, CCR5, CCR10, XCR1, CXCR1, IL-8R, CXCR4, PDGFRB, KDR, CD44, IFNAR1 and TEK) increased two- to eighteen-fold higher levels. The cytokine analysis revealed that the ligand levels of hAT-MSCs (medulloblastoma: VEGF, IL6, IL8, CCL3, CCL2 and TEK; ATRT: VEGF, CCL3 and TEK; glioblastoma: CCL2, PDGF, TEK and IGF1) and the ligand levels BTICs (medulloblastoma: CCL5 and CXCL4; ATRT: IL-8 and CXCL4; glioblastoma: CXCL4 and IGF1) were elevated in the co-cultured media. Our findings demonstrated that hAT-MSCs can target BTICs. Cross-talk between hAT-MSCs cytokine receptor and BTICs ligand (medulloblastoma: CCR5/CCL5 and CXCR4/CXCL4; ATRT: IL-8R/IL-8 and CXCR4/CXCL4; glioblastoma: CXCR4/CXCL4 and IGF1R/IGF1) play cardinal role in this process. Citation Format: Seung Ah Choi, Kyu-Chang Wang, Ji Hoon Phi, Ji Yeoun Lee, Jung Won Choi, Hyung Ah Kim, Se Hee Kim, Yong Hwy Kim, Sung Eun Kwon, Young-Hoon Kim, Seung-Ki Kim. Human adipose tissue-derived mesenchymal stem cells can target brain tumor initiating cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2649. doi:10.1158/1538-7445.AM2013-2649
OP0157 Use of Biologics in Patient with Juvenile Idiopathic Arthriitis
Authors: G. Horneff, K. Minden, H. I. Huppertz
Journal: Annals of the Rheumatic Diseases (2013)

Background Only few biologics are approved for treatment of juvenile idiopathic arthritis (JIA) while numerous drugs were used additionally on an off label use. Objectives To ananlyse the primary and the sequential use of biologics in JIA patients. Methods Analysis of the courses and reasons for discontinuation of biologics treatment in the German BIKER registry. Results A total of 2566 treatment courses with biologics have been documented in 2016 JIA patients. 1583 patients received one biologic, 337 two, 77 three, 18 four and 1 patient received 6 different biologic agents. Thus 21.5% only switched from one to at least a second biologic. As expected, etanercept is the most commonly used agent (n=1778), followed by adalimumab (463), tocilizumab (81), anakinra (61), abatacept (57), infliximab (48), golimumab (28), and canakinumab (14). Thus, etanercept is the most commonly used biologic and 60% of patients treated with adalimumab have first tried etanercept. The kind and number of first line biologic, ever used biologic and last biologic agent used is given in [Table 1][1]. Of interest, these numbers differ markedly in JIA patients with systemic arthritis and in JIA patients with uveitis history. Adherence (ratio of last to ever used biologic) in all JIA patients was highest in tocilizumab (95%) followed by etanercept (79%), adalimumab (79%), abatacept (68%), anakinra (64%) and canakinumab (50%). In systemic JIA adherence was higher in tocilizumab (90%) than with anakinra (67%), canakinumab (62%) followed by with TNF-inhibitors (50%> 62%) and lowest for abatacept (25%). Inefficacy was the most common reason for discontinuation of biologics (13%) followed by remission (11.3%) and intolerance (4.8%). Thus only few patients did not tolerate treatment with biologics. Inefficacy led to discontinuation of abatacept in 12(21.1%), etanercept in 283 (15.6%), canakinumab in 2 (14%), adalimumab in 31 (6.7%), tocilizumab in 3 (4%), infliximab in 1 and golimumab in 2 (4%). ![Figure][2]</img> Conclusions JIA patients are exposed to a number of different biologics. The majority of patients were still exposed to a single biologic. Inefficacy is the most common reason for discontinuation while intolerance is infrequent. The use of anakina, canakinumab and tocilizumab is more frequent in systemic JIA patients, TNF inhibitors were still used in the majority of patients. Since participation on the registry is voluntary, off label use might be underestimated. Disclosure of Interest G. Horneff Grant/research support from: Pfizer, Abbott, Roche, K. Minden Grant/research support from: Pfizer, Abbott, H. Huppertz: None Declared [1]: #F1 [2]: pending:yes
HEMODYNAMIC EFFECTS OF PULMONARY VALVE REPLACEMENT FOR PULMONARY REGURGITATION AFTER REPAIR OF ISOLATED PULMONARY STENOSIS: A MATCHED COMPARISON WITH TETRALOGY OF FALLOT
Authors: Jouke Pieter Bokma, Michiel Winter, Thomas Oosterhof, Hubert Vliegen, Arie Van Dijk, Mark Hazekamp, David Koolbergen, Maarten Groenink, Barbara Mulder, Berto Bouma
Journal: Journal of the American College of Cardiology (2015)
Scintigraphic and Radiographic Appearance of Osteomas in Gardner’s Syndrome
Authors: BOHDAN BYBEL, ADEL G. MATTAR
Journal: Clinical Nuclear Medicine (2003)

From the Department of Molecular and Functional Imaging,* Cleveland Clinic Foundation, and the Department of Nuclear Medicine, † London Health Sciences Centre, London, Ontario, Canada Received for publication June 19, 2002. Revision accepted September 18, 2002. Correspondence: Bohdan Bybel, Department of Molecular and Functional Imaging, Cleveland Clinic Foundation, 9500 Euclid Avenue Gb3, Cleveland, Ohio 44195. E-mail: [email protected]
Doughnut Appearance on I-131 MIBG Scintigraphy
Authors: ZEHRA ÖZCAN, SERDAR ERDEM, HAYATI AKIN, ZEYNEP BURAK, HAYAL ÖZKILIÇ
Journal: Clinical Nuclear Medicine (1996)

The uptake of I-131 MIBG in neuroblastoma is well-established in imaging studies. I-131 MIBG scintigraphy of a patient with neuroblastoma illustrated “doughnut” appearance, which is a well-recognized bone scan finding mostly in giant-cell tumor of bone. This finding was probably caused by central necrosis and hemorrhage, and surrounding viable tumor tissue.
Abstract 3138: Epigenetic regulation of Src pathway in pancreatic adenocarcinomas
Authors: Elena Padin-Iruegas, Elena Evgenyeva, Michel Herranz, Ihab Abdulkader, Elena Brozos, Maria Pombo, Jose Ramón Antúnez, Alvaro Ruibal, Rafael López
Journal: Cancer Research (2012)

Abstract Purpose: Src cascade is crucial in tumor growth and metastasis development. Nowadays, one of the most relevant mechanisms for gene control is epigenetic mechanisms, both methylation and acetylation. We study the epigenetic regulation of Src pathway in pancreatic adenocarcinomas. The main purpose of this study was to obtain translational results, we can apply in the clinical practice. Methods: 70 pancreatic adenocarcinomas and normal tissue from the same patient were analyzed. DNA from laser microdissected paraffin embedded sample was obtained, bisulfite treated, methylation specific PCR (MSP) amplified, and agarose visualized. Some cases were bisulfite sequenced for verification. Rassf1a, Cox2, Socs, E-Cadherin, DAP-kinase, TIMP3, TWIST1, p16 and Cyclin-D2 genes were analyzed due to their implication with Src pathway. All patients signed informed consent and detail clinic histories were reviewed. Results: We found gene hypermethylation in all the selected targets ranged between 23.5% (DAP-K) to 73% (TWIST1), i.e. RASSF1α (48.7%); p16 (38.2%)… Bisulfite promoter sequence confirms MSP results. Patient clinical profile clustered in some well defined groups. Epigenetic profile also showed very nice association. Combine clinical and epigenetic profile showed a specific and characteristic correlation between some clinical parameters and specific gene methylation profile. In this sense, for instance, we found a perfect match between just RASSF1α promoter hypermethylation with clinical parameters: pTNM and tumor localization. Conclusions: Gene hypermethylation control seems important in pancreatic adenocarcinoma behavior. Src pathway could be implicated in tumoral development, both directly and indirectly (through i.e. EGFR, HER2/Neu, Integrins and SPARC). Src pathway methylation control could be important in development and progression of pancreatic carcinoma (up to 73%). An “epigenetic fingerprint” of pancreatic adenocarcinoma was found from clinical and epigenetic clustering analysis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3138. doi:1538-7445.AM2012-3138
AB1056 Aortic calcification on lumbar spine radiographs: Is it important?
Authors: F.A.B. Ibrahim, L. Durcan, G. Cunnane
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Vascular calcification is a marker of endothelial cell damage and is associated with cardiovascular morbidity. Calcification of the abdominal aorta (AA) is occasionally noted as an incidental finding on lumbar spine x-rays, but the significance of this observation is unknown. We undertook a study of patients with osteoarthritis (OA) or rheumatoid arthritis (RA) who underwent lumbar spine radiography for evaluation of lower back pain. We used available laboratory data to investigate any possible links between AA calcification and cardiometabolic risk factors. <h3>Methods</h3> Chart reviews were performed on sequential patients with RA and OA attending rheumatology out-patient clinics. Those who had recent plain films of their lumbosacral spine were included in our analysis. Co-morbid conditions, medication use, available laboratory and radiology results were recorded. Each lumbar spine x-ray was evaluated for aortic calcification by 2 investigators (FI and GC) using the following scoring system: <i>nil:</i> no calcification; <i>minimal:</i> calcification measuring less than the height of 1 vertebral body; <i>moderate:</i> calcification measuring &lt;2 vertebral bodies; <i>extensive:</i> calcification measuring &gt;2 vertebral bodies. Statistical assessment was performed using SPSS version 18. Categorical variables were compared using a chi squared test and continuous variables were compared using the students t test. <h3>Results</h3> 214 patients were included in the analysis of which 144 were female. Mean age was 65.7 years (range 40 to 92).RA was the underlying diagnosis in 110, while 104 had OA. No patient had been diagnosed with gout. There was no statistically significant difference between these groups in terms of age, gender, vascular calcification, body mass index (BMI), dyslipidemia or hyperglycemia. However, hyperuricemia was more prevalent in the OA group (p=0.023). Almost half the total cohort (47.2%) had evidence of moderate or extensive AA calcification. As expected, the incidence of vascular calcification increased with age (p&lt;0.00001). AA calcification also correlated significantly with hyperuricaemia (P&lt;0.04), but not with other cardiometabolic risk factors such as BMI or dyslipidemia. <h3>Conclusions</h3> Aortic calcification increased with age and was associated with hyperuricemia, but not other laboratory-based cardiometabolic factors. Chronic inflammatory disease did not confer additional risks for vascular calcification. Hyperuricemia, known to have adverse effects on vascular health, correlated significantly with AA calcification and may therefore have a similar deleterious effect on blood vessels as chronic inflammation. <h3>Disclosure of Interest</h3> None Declared