tbuckley/cabot-search huggingface dataset:
Abstract 340: Squamous cell carcinoma in the mouse: A novel, rapidly growing, syngeneic orthotopic lung tumor model .
Authors: Moshe Giladi, Rosa S. Schneiderman, Yaara Porat, Mijal Munster, Aviran Itzhaki, Daniel Mordechovich, Shay Cahal, Eilon D. Kirson, Yoram Palti
Journal: Cancer Research (2013)

Abstract Lung cancer is the leading cause of cancer death and accounts for 28% of all cancer death in the United States. About 80% of lung cancers are non-small cell lung carcinoma (NSCLC) of which over 20% are Squamous Cells Carcinoma (SCC). The currently available therapies, for NSCLC, are less effective against squamous cells carcinoma. While there are several established in-vivo models for lung adenoma and adenocarcinomas, there are no rapidly growing, syngeneic, orthotopic tumor models for lung SCC. The goal of the present study was to develop such a model in order to facilitate research of new treatments against SCC. Murine SCC (KLN205) cells were mixed with matrigel and injected directly into the lungs of C57BL/6 and DBA-2 mice using the same methodology applied during the induction of Lewis Lung Carcinoma orthotopic tumors. Injection of KLN205 resulted in development of highly variable tumors with an average size of 24mm3 in DBA-2 mice after 3 weeks from inoculation. No sustainable tumors were seen in C57BL/6 mice. Cells derived from DBA/2 lung tumors (referred to as KLN205-T1) were subcultured for over 10 generations and their cytological characteristics were determined. A second round of orthotopic tumor injections using KLN205-T1 cells resulted in the formation of reproducible, solitary and localized tumors in the lungs of over 85% of the DBA/2 mice but not in C57BL/6 mice. The tumors increased in average size from 20 to 40 to 80 mm3 between days 10, 13 and 16. Comparable results were obtained while imaging tumor progression using MRI. Metastases were observed only in thoracic lymph nodes which were probably a result of cells escaping the lung during tumor injection. Immunostaining of KLN205-T1 tumors with p63 and cytokeratin 5/6 demonstrated similar characteristics as described for human SCC. Cisplatin and paclitaxel were tested in this KLN205-T1 tumor model demonstrating its potential use for testing treatment options for SCC lung cancer. The effect of Tumor Treating Fields (TTFields) therapy with and without paclitaxel or cisplatin is currently being tested in this novel tumor model. Citation Format: Moshe Giladi, Rosa S. Schneiderman, Yaara Porat, Mijal Munster, Aviran Itzhaki, Daniel Mordechovich, Shay Cahal, Eilon D. Kirson, Yoram Palti. Squamous cell carcinoma in the mouse: A novel, rapidly growing, syngeneic orthotopic lung tumor model . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 340. doi:10.1158/1538-7445.AM2013-340
P80 Characterisation Of The Eq-5d-5l And Exercise Performance In Bronchiectasis
Authors: S. Greenwood, T. Pemberton, L. Grillo
Journal: Thorax (2014)

<h3>Introduction and objectives</h3> NHS England have been looking at using the EQ-5D-5L as a measure of health outcome across the NHS. It is a simple measure which patients complete at the start and end of treatment to evaluate quality and effectiveness of interventions. To date there is no evidence on its use in Bronchiectasis (Bx). Moreover, evaluation of exercise performance is also vital as this can be associated with increased dyspnoea, reduced lung function or increased malaise. Sit to stand (5STS) and six minute walk test (6MWT) can be used to evaluate exercise performance but there is limited guidance on responsiveness and feasibility in Bx. This abstract provides novel data for these outcome measures (OM) in Bx patients during a routine inpatient stay. <h3>Methods</h3> 20 Bx inpatients (Male: Female 20:20, Median age: 63 (29–74) Median FEV<sub>1</sub>: 1.26 (0.51–2.9) were assessed. 6MWT, 5STS and EQ-5D-5L were completed on all patients during their initial and final assessment. <h3>Results</h3> Median length of stay was 10 days. Data is presented as median difference and comparisons were made using Wilcoxon Signed Rank tests. <h3>Conclusion</h3> The EQ-5D-5L improved but did not show a significant difference, moreover there is currently no reported MCID for this OM. Significant differences were seen in both the 6MWT and 5STS. The 5STS is quick and feasible to complete and therefore maybe more preferable to use than the 6MWT. More understanding is needed on the utility of the EQ-5D-5L in this population. <h3>References</h3> Pasteur <i>et al</i>. 2010 ‘BTS Guidelines for non-CF Bronchiectasis’ EQ-5D-5L (www.euroquol.org)
Abstract 4745: A whole genome association study and identification of a locus for histiocytic sarcoma in the flat-coated retriever
Authors: Abigail L. Shearin, Emmett V. Schmidt, Heidi G. Parker, Cathryn S. Mellersh, Matthew Breen, Elaine A. Ostrander
Journal: Cancer Research (2010)

Abstract Histiocytic sarcoma (HS) is a cancer characterized by unregulated proliferation of histiocytes. In the flat-coated retriever (FCR), the primary tumor site is frequently appendicular with a high rate of local and distant metastases. The disease occurs with a high prevalence in the FCR, accounting for approximately 40% of all cancers in the breed and 18% of disease-related deaths in a UK cohort. Breed-specific prevalence in FCRs indicates a strong genetic predisposition. Our goal is to identify the underlying loci and causal mutations associated with HS in the FCR. A whole genome association study (GWAS) using the Illumina Canine SNP20 BeadChip was performed using 98 DNA samples from FCR collected from the United States (48 cases and 50 unaffected controls over the age of ten yrs). Of the 23,000 SNPs genotyped, 17,000 were informative in the FCR. A single-marker chi-squared analysis using EMMA to correct for population structure and kinship yielded an association with the HS phenotype (p=2.33×10-5). Including the flanking markers, the associated region spans 12 Mb and includes several candidate genes of interest. To reduce this critical interval, ABI SNPlex genotyping and candidate gene sequencing is in progress. Our current data distinguish HS in the FCR from the related disease malignant histiocytosis (MH) found in Bernese mountain dogs (BMD). MH is a disseminated histiocytic cancer with primary tumor sites usually occurring in visceral organs. Our data suggest that HS in the FCR is not a localized form of MH. GWAS analyses for both breeds indicate that the major contributing heritable factors of MH in the BMD differ from those for HS in the FCR. Given the different behaviors of the cancers between the two breeds and the different heritable loci associated with them, our current evidence distinguishes them as separate diseases. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4745.
Laparoscopic resection of a gastric glomangioma
Authors: Xiao-jun Zhao, Hang-hong Wang, Jian-qiu Sheng, Na Li
Journal: Endoscopy (2014)

A 51-year-old woman underwent gastroscopy because of epigastric distension. The mucosa in the lesser curvature of the distal gastric body showed a hemispheric bulge, with a smooth surface and normal color ([Fig. 1]). An ultrasound scan showed the presence of a lesion located within the submucosa. It was olive shaped with a medium echo, and the boundary was distinct and smooth with uneven echoes. Multiple hypoechoic lumen-like structures, which typically measured approximately 2 mm, were observed inside the lesion. Color Doppler showed a rich blood flow inside these lumen-like structures ([Fig. 2]).
Role of Receptor Protein Tyrosine Phosphatase γ in Sensing Extracellular CO2 and HCO3 −
Authors: Yuehan Zhou, Lara A. Skelton, Lumei Xu, Margaret P. Chandler, Jessica M. Berthiaume, Walter F. Boron
Journal: Journal of the American Society of Nephrology (2016)

Regulation of blood pH-critical for virtually every facet of life-requires that the renal proximal tubule (PT) adjust its rate of H(+) secretion (nearly the same as the rate of HCO3 (-) reabsorption, JHCO3 ) in response to changes in blood [CO2] and [HCO3 (-)]. Yet CO2/HCO3 (-) sensing mechanisms remain poorly characterized. Because receptor tyrosine kinase inhibitors render JHCO3 in the PT insensitive to changes in CO2 concentration, we hypothesized that the structural features of receptor protein tyrosine phosphatase-γ (RPTPγ) that are consistent with binding of extracellular CO2 or HCO3 (-) facilitate monitoring of blood CO2/HCO3 (-) concentrations. We now report that PTs express RPTPγ on blood-facing membranes. Moreover, RPTPγ deletion in mice eliminated the CO2 and HCO3 (-) sensitivities of JHCO3 as well as the normal defense of blood pH during whole-body acidosis. Thus, RPTPγ appears to be a novel extracellular CO2/HCO3 (-) sensor critical for pH homeostasis.
FDG PET/CT Images Demonstrating Epididymo-Orchitis in a Patient With HIV, Acute Kidney Injury and Known Epididymo-Orchitis on Scrotal Ultrasound
Authors: Sameer Chopra, Arjuna Dharmaraja, Pareen Mehta, Patrick M. Colletti, Heidi Wassef
Journal: Clinical Nuclear Medicine (2014)

A 52-year-old man with HIV was referred for an F-FDG PET/CT scan for the cause of kidney injury. FDG PET/CT scan revealed increased renal cortical FDG activity, which can be seen in HIV nephropathy or acute interstitial nephritis. Diffuse increased FDG uptake was demonstrated within the right testicle and epididymis, consistent with the patient's known right epididymo-orchitis, as diagnosed on ultrasound 1 week before admission. Multiple enlarged lymph nodes with increased FDG activity were also found within the right inguinal and external iliac nodal chains, which were presumed to be reactive. The patient was treated with ciprofloxacin with symptomatic improvement.
Comparison between HER-2/neu, estrogen and progesterone receptors in primary breast carcinomas and matched lymph node metastases.
Authors: MM Desouki, SE Self, DJ Wolff
Journal: Cancer Research (2009)

Abstract Abstract #3053 Background: HER2, estrogen (ER) and progesterone receptors (PR) are important markers for prognostic and therapeutic implications in breast cancer. Fluorescence in situ hybridization (FISH) and IHC for HER2 gene amplification and evaluation of ER and PR in primary tumor are commonly used methods. However, evaluation of these markers in lymph nodes or distant metastases, which may be more biologically significant, is not that applicable in regular clinical basis. In the current work we compared HER2 (by FISH), ER and PR (by IHC) in matched primary breast carcinomas and lymph node metastases.&amp;#x2028; Design: Formalin fixed, paraffin embedded tissue sections from 39 cases of primary and lymph node metastases were assessed for Her2 (by FISH). Primary tumors of the cases selected were known to be HER2 un-amplified. Also, ER and PR IHC were performed on 36 cases from the same cohort of cases to assess any discrepancy between primary and lymph node metastases of these cases.&amp;#x2028; Result: Out of 39 cases, one case was HER2 amplified in lymph node metastasis compared to un-amplified primary tumor. Eight percent of cases (3/36) were ER negative in LN mets and 6% (2/36) were less strongly positive compared to the positive primary tumors. Nineteen percent (7/36) were PR negative in LN mets in contrast to the matched positive primary tumors. Regarding PR immunoreactivity, 6% (2/36) were PR-positive in LN met compared to their corresponding negative primary tumors.&amp;#x2028; Conclusion: While most matched primary breast tumors and lymph node metastases show concordance in HER2, ER, PR status, we found discordance in a minority of cases. These results suggest the possibility that extension of studies in metastatic breast tumors in parallel with that of primary lesions is informative and can be used to determine further treatment options and predict prognosis in breast cancer patients. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 3053.
1150 HEPATITIS C VIRUS LIVER TRANSPLANTATION ESCAPE VARIANT IS CHARACTERIZED BY BOTH ENHANCED TRIGLYCERIDE-RICH LIPOPROTEIN ASSOCIATION AND SENSITIVITY TO apoE ANTIBODIES
Authors: D.J. Felmlee, C. Fauvelle, L. Heydmann, M.-S. Hiet, I. Fofana, R. Bartenschlager, F. Stoll-Keller, M.B. Zeisel, S. Fafi-Kremer, T.F. Baumert
Journal: Journal of Hepatology (2013)
Abstract 5174: HYAL4: a new player in bladder cancer progression and diagnosis
Authors: Diogo O. Escudero, Judith Knapp, Soum Lokeshwar, Murugesan Manoharan, Mark Soloway, Vinata B. Lokeshwar
Journal: Cancer Research (2011)

Abstract BACKGROUND: Hyaluronidase (HAase) family of enzymes degrades hyaluronic acid (HA). In the human genome there are 6 HAase genes clustered on chromosomes 3p21.3 (HYAL-1, -2, -3) and 7q31.3 (HYAL-4, PH20, HYALP1). While PH20 and HYAL-2 are testicular and lysosomal HAases, HYAL1 is a tumor-derived HAase. HYAL1 promotes tumor growth, invasion, and angiogenesis and is a diagnostic and prognostic marker for bladder cancer (BCa). The expression of other HAases has not been investigated in either normal cells or tumor cells and tissues. In this study we examined the expression of all 6 HAases in bladder tissues, cells and urine. METHODS: Using time RT-PCR, we examined the mRNA levels of 6 HAase genes in BCa cell lines, 59 bladder tissues (normal (NBL) = 22; tumor (TBL) = 37) and 160 urine specimens (BCa = 52; normal = 18; history of BCa = 30; benign conditions = 59). The levels were normalized to β-actin. HYAL1 and HYAL4 were localized in bladder tissues by immunohistochemistry using anti-HYAL1 and anti-HYAL4 antibodies, respectively. The staining was graded for intensity (0 – 3) and area of staining. HYAL4 function was analyzed by siRNA transfection of HT1376 and HT5637 cell lines. RESULTS: HYAL1, HYAL4 and HYALP1 mRNA levels were significantly (6-13-fold) elevated in TBL tissues (12.5 ± 2.7; 29.7 ± 21.9; 26.7 ± 21.9) when compared to NBL tissues (1.2 ± 0.6; 2.2 ± 2.4; 4.2 ± 6.6; P 0.05). cDNA cloning revealed that HYALP1 is a pseudo gene that encodes a non-functional truncated protein. HYAL1 and HYAL4 staining was significantly elevated in TBL tissues (188 ± 57; 232 ± 91) when compared to NBL tissues (68.5 ± 21.3; 93 ± 46; P &amp;lt; 0.001). The staining for both HAases was exclusively localized in tumor cells. When compared to HYAL3, PH20, and HYAL2 mRNA levels, HYAL1, HYAL4 and HYALP1 mRNA levels were significantly elevated in BCa patients’ urine (P &amp;lt; 001). Urinary HYAL4 mRNA levels had higher sensitivity (78.9%) and specificity (86.2%) than HYAL1 mRNA levels (72.3%; 76.2%) to detect BCa. Both HAases detected high-grade BCa with 83% – 86% sensitivity. Down regulation of HYAL4 expression by siRNA decreased proliferation of HT1376 and HT5637 cells (2.3-fold) and induced apoptosis (2.6-fold). Conclusion: This is the first study showing that, like HYAL1, HYAL4 is a tumor derived HAase that is exclusively expressed in BCa cells. HYAL4 may be a potentially accurate marker for BCa diagnosis and may promote BCa growth and progression. Support: Grant NCI/NIH R01 CA-72821-09; Bankhead Coley Cancer Research Program Sylvester Comprehensive Cancer Center and CURED Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5174. doi:10.1158/1538-7445.AM2011-5174
P66 IMPDH2-TARGETED CONSTRAINT OF CELL GROWTH IN HEPATOCELLULAR CARCINOMA BY MYCOPHENOLIC ACID
Authors: K. Chen, K. Man, H.J. Metselaar, H.L.A. Janssen, J. Kwekkeboom, D. Sprengers, M.J. Bruno, M.P. Peppelenbosch, Q. Pan
Journal: Journal of Hepatology (2014)
Calcified Object in the Mid-Maxilla
Authors: Siegfried A Schwab, Rolf Janka
Journal: New England Journal of Medicine (2010)

A 60-year-old woman underwent multislice whole-body computed tomography for staging of a high-risk melanoma of the anus. The examination revealed no metastases but did reveal a round, calcified structure in the mid-maxilla (Panel A, arrow). In the sagittal reconstruction, a peg-shaped supernumerary tooth was identified (Panel B, arrow), which resulted in the diagnosis of an unerupted, inverted mesiodens. A mesiodens is a developmental disturbance that occurs during odontogenesis; the overall prevalence is 0.2 to 1.9%. A mesiodens can cause median diastema (an open space between the incisors), a dentigerous cyst (a cyst associated with the crown of an unerupted or . . .
Evaluation of MDS and ADAC Edge Detection Algorithms on Simultaneously Acquired Data:
Authors: V.J. Ficken, E. W. Allen, M.F. Wilson, C. J. Ryals
Journal: Clinical Nuclear Medicine (1983)

Ficken, V.J. M.S.; Allen, E. W. M.D.; Wilson, M.F. M.D.; Ryals, C. J. Author Information
Increased Expression of an Adhesion-Promoting Surface Glycoprotein in the Granulocytopenia of Hemodialysis
Authors: M. Amin Arnaout, Raymond M. Hakim, Robert F. Todd, Nava Dana, Harvey R. Colten
Journal: New England Journal of Medicine (1985)

To identify the mechanisms accounting for hemodialysis-induced granulocytopenia, we undertook quantitative kinetic studies of a granulocyte-adhesion–promoting surface glycoprotein (Mo1). In eight patients undergoing maintenance hemodialysis, there was a fivefold increase in the mean cell-surface expression of Mo1 within 15 minutes after the start of dialysis with a new cuprophane membrane. The peak increase in surface Mo1 coincided with the maximal drop in neutrophil count and with the peak rise in the plasma levels of the complement-activation products C5a desArg and C3a desArg. During dialysis on a membrane being reused for the fifth time, no significant complement activation, no increase in Mo1 expression, and no change in neutrophil count were seen. C5a desArg (but not C3a desArg) induced a comparable increase in Mo1 expression on normal granulocytes in vitro at concentrations similar to those measured in vivo. Chemotactic peptide–induced granulocyte aggregation (a reflection of increased cell-to-cell adhesiveness) was specifically blocked by mouse monoclonal antibodies to Mo1 in vitro. These data suggest that the increased expression of Mo1 on granulocytes in vivo is in part mediated by C5a (and C5a desArg). The quantitative increase in granulocyte-surface Mo1 may provide a mechanism for initiating leukoaggregation, sequestration of granulocytes, and neutropenia during hemodialysis. (N Engl J Med 1985; 312:457–62.)
Tumor Response and Secondary Resectability of Colorectal Liver Metastases Following Cetuximab with Chemotherapy: A Randomized, Controlled Study
Authors: L. Ye, D. Zhu, L. Ren, Y. Wei, X. Pan, Y. Zhong, L. Liang, B. Xu, T. Liu, J. Xu
Journal: Annals of Oncology (2012)
Abstract 1295: Inhibition of platelet derived growth factor receptor tyrosine kinase reduces lymphangiogenesis of human gastrointestinal cancer
Authors: Michiyo Kodama, Yasuhiko Kitadai, Tomonori Sumida, Mayu Ohnishi, Eiji Ohara, Kei Shinagawa, Miwako Tanaka, Shinji Tanaka, Wataru Yasui, Kazuaki Chayama
Journal: Cancer Research (2010)

Abstract Objective: Vascular endothelial growth factor (VEGF)-C/D induces lymphangiogenesis by activating VEGF receptor (VEGFR)-3, which is expressed on lymphatic endothelial cells. Platelet derived growth factor receptor (PDGF-R) has also been detected on lymphatic endothelial cells and PDGF-BB acts as a lymphangiogenic factor in murine fibrosarcoma. We have previously reported that PDGF-R is expressed by lymphatic endothelial cells in human gastric cancer and its expression levels in tumor tissue correlate with lymph node metastasis. In this study, we examined whether inhibition of PDGF-R tyrosine kinase signaling by imatinib affects lymphangiogenesis and lymph node metastasis of human gastrointestinal cancer cells growing in the orthotopic site of nude mice. Methods: TMK-1 human gastric cancer cells or KM12SM human colon cancer cells were injected into the orthotopic site of nude mice. Groups of mice (n=10) received saline (control), imatinib, the cancer chemotherapeutic irinotecan, or a combination of imatinib and irinotecan. The lymphatic vessels were then stained with antibodies against Lyve 1. Results: Lymphatic endothelial cells as well as activated fibroblasts in the tumor stroma express PDGF-R. Four weeks of treatment with imatinib and irinotecan significantly inhibited lymph node metastasis (relative to control or single-agent therapy). Imatinib alone or in combination with irinotecan significantly decreased number and area of lymphatic vessels in the tumor stroma. Conclusion: Administration of a PDGF-R tyrosine kinase inhibitor in combination with irinotecan inhibits the lymph node metastasis of orthotopically implanted gastrointestinal cancer cells in nude mice by blocking PDGF-R signaling in tumor-associated lymphatic endothelial cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1295.
SAT0238 Risk of Falls in Patients with Ankylosing Spondylitis
Authors: N. Dursun, S. Sarikaya, S. Ozdolap, E. Dursun, C. Zateri, L. Altan, M. Birtane, K. Akgun, A. Revzani, I. Aktas, N. Tastekin, R. Celiker
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Risk of vertebral fractures is increased in patients with ankylosing spondylitis (AS). The underlying mechanisms for the elevated fracture risk might be associated with bone and fall-related risks. <h3>Objectives</h3> The aim of this study was to evaluate the risk of falls and to determine the factors that increase the risk of falls in AS patients. <h3>Methods</h3> Eighty-nine women, 217 men, a total of 306 AS patients with a mean age of 40.1 ± 11.5 from 9 different centers in Turkey were included in the study. Patients were questioned regarding history of falls within the last one year. Their demographics, disease characteristics including Bath AS Disease Activity Index (BASDAI), Bath AS Metrology Index (BASMI), Bath AS Functional Index (BASFI), AS Quality of Life Index (ASQoL), and risk factors for falls including Vitamin D levels and Short Physical Performance Battery (SPPB) Test results were recorded. <h3>Results</h3> Forty (13%) out of 306 patients reported at least one fall in the recent one year. Statistically significant differences were found between the patients with and without a history of falls regarding age (p=0,001), disease duration (p=0,001), history of fractures (p=0.035), fear of falls (p=0,000), hip involvement (p=0,032), BASMI (p=0,002), BASFI (p=0,000), and SPPB (p=0,000). No significant correlation were found between number of falls and gender, morning stiffness, polypharmacy, knee involvement, ankle involvement, usage of antidepressants or myorelaxants, AS related days of sick leave, BASDAI, ASQoL, sedimentation, CRP, and Vitamin D levels (p&gt;0,05 for all parameters). Statistically significant correlations were found between number of falls and age (R=0,117, p=0,041), disease duration (R=0,160, p=0,005), AS related lost job (R=0,140, p=0,014), fear of falling (R=0,316, p=0,000), hip involvement (R=0,112, p=0,05), BASMI (R=0,234, p=0,000), BASFI (R=0,244, p=0,000), and SPPB (R=0,183, p=0,006). <h3>Conclusions</h3> The annual incidence of falls was higher among AS patients than that of normal population. Age, longstanding disease, hip joint involvement, fear of falls, history of fractures, reduced mobility, decreased function, impaired balance were found to be the factors that increase the risk of falls in AS patients. <h3>References</h3> Geusens P, Vosse D, Van der Linden S: Osteoporosis and vertebral fractures in ankylosing spondylitis. Curr Opin Rheumatol 2007, 19:335-339. Sambrook PN, Geusens P: The epidemiology of osteoporosis and fractures in ankylosing spondylitis. Ther Adv Musculoskel Dis 2012, 4(4): 287-292. <h3>Disclosure of Interest</h3> None Declared