tbuckley/cabot-search huggingface dataset:
FRI0058 Production of An Anti-Type II Collagen IGG2B Monoclonal Antibody Capable of Inducing Destructive Polyarthritis in Mice
Authors: S. Yoshino, N. Mizutani, T. Koobkokkruad
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> A mouse model of destructive antibody-mediated polyarthritis (AMPA) induced by a combination of four or five different monoclonal antibodies (mAbs) specific for type II collagen (CII) was previously established. However, it has not been well defined whether an anti-CII mAb is able to induce AMPA. <h3>Objectives</h3> The present study aimed to produce an anti-CII mAb that induced destructive AMPA in mice. <h3>Methods</h3> To establish anti-CII mAb-producing hybridomas, DBA mice were repeatedly sensitized with chicken CII plus CFA and then CII-sensitized spleen cells were fused with myeloma cells before cloning fused-cells producing mAbs against mouse CII. To induce arthritis, DBA mice were i.p. injected with various doses of each produced anti-CII mAb (day 0), followed by i.p. administration of LPS on day 3. To evaluate the severity of the arthritis, lesions of the four paws were each graded from 0 to 3 according to the increasing extent of erythema and edema of the periarticular tissue. Histological analysis of inflamed joints and measurement of C3 in serum were also performed. <h3>Results</h3> A number of hybidomas producing different isotypes of anti-CII mAbs including IgG1, IgG2a, IgG2b, IgG3, and IgM were established. I.p. injection of various doses of each anti-CII mAb into DBA mice revealed that only two mAbs (C2B-9 and C2B-14) induced arthritis by the injection without a combination of the mAbs. The incidence of AMPA was 100% in the 5 mice injected with either C2B-9 or C2B-14. However, C2B- 9 induced only mild arthritis while C2B-14 induced moderate joint inflammation, although injection of both C2B-9 and C2B-14 induced severe arthritis. The isotype of C2B-9 and C2B-14 was IgG2b. Histologically, marked hyperplasia of the synovium as well as destruction of cartilage and bone was observed in mice injected with C2B-14 but not C2B-9. Significant decreases in C3 in serum were also observed in C2B-14- but not in C2B-9 -injected animals. <h3>Conclusions</h3> We successfully produced an anti-CII IgG2b mAb, C2B-14, that induced destructive AMPA. The destructive AMPA by C2B-14 was associated with low levels of C3 in serum, suggesting a role of complement activation in C2B-14-induced AMPA. <h3>References</h3> Terato, K., et al., J. Immunol. 148: 2103, 1992. Nandakumor, K.S., et al., J. Immunol. Methods 304: 126. 2005. Hutamekalin, P., et al., J. Immunol. Methods 343: 49, 2009. <h3>Acknowledgement</h3> This work was supported by a Grant-in-Aid for Scientific Research (C) from the Japan Sciety for the Promotion of Science. <h3>Disclosure of Interest</h3> None declared
Factors Predicting Gvhd Free, Relapse Free Survival (GRFS) after Allogeneic Hematopoietic Cell Transplantation; A Multivariable Analysis of 531 Allografts from a Single Center
Authors: Melhem Solh, Xu Zhang, Katelin Connor, Stacey Brown, H. Kent Holland, Lawrence E. Morris, Scott R. Solomon, Asad Bashey
Journal: Blood (2015)
SAT0465 Sensorimotor Performance and Function in People with Osteoarthritis of The Hand: A Case-Control Comparison
Authors: N. Magni, P. McNair, D. Rice
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Hand osteoarthritis (OA) is a common cause of pain and disability, affecting 20% of adults over age 70 [1]. Classically this pathology has been attributed solely to impairments of peripheral structures (e.g. joint and surrounding tissues). However, recent evidence has shown that changes in the central nervous system may contribute to the maintenance of pain and disability [2]. To date no evidence has focused on brain related sensorimotor impairments in hand OA, highlighting the need for further studies on this topic. We hypothesised that patients with hand OA would present with evidence of sensorimotor impairments and that these changes would be correlated to hand function. <h3>Objectives</h3> To determine whether motor imagery (MI), tactile acuity, and body perception, are disrupted in people with hand OA. To verify if a correlation exists between these measures and hand function in people with hand OA. <h3>Methods</h3> Twenty patients with symptomatic hand OA and 19 healthy pain-free controls undertook MI (hand left/right judgments), a brain speed processing task (controls left/right judgments), two-point discrimination (TPD) threshold testing, a neglect-like symptoms questionnaire (assessing body perception) and hand performance tests. <h3>Results</h3> People with hand OA were slower and less accurate in the MI task (<i>P</i> &lt;0.05) when compared to healthy controls, with no brain processing differences between groups. No difference was found between groups for two-point discrimination measures. Patients with hand OA presented with higher (<i>P</i> &lt;0.05) scores on the neglect-like questionnaire. Interestingly, people with left hand pain demonstrated enhanced neglect-like symptoms (<i>P</i> &lt;0.05) compared to those with right-sided hand pain. A correlation between TPD thresholds and measures of hand performance was identified, however no relation was found between MI and hand function. <h3>Conclusions</h3> Patients with hand OA demonstrate brain related sensorimotor deficits. Additionally, the side of the pain appears to influence body perception, probably due to differences in right and left hemispheric functions. Sensorimotor measures partially correlate to function, specifically tactile acuity is moderately related to hand function. <h3>References</h3> Zhang, Y. et al. Am J Epidemiol.2002:156:1021–7. Arendt-Nielsen, L. et al. Pain.2010:149:573–81. <h3>Disclosure of Interest</h3> None declared
PTH-130 Attitudes of Medical Students Towards Gastroenterology Teaching in The UK: A BSG Survey
Authors: VTF Cheung, GJ Johnson
Journal: Gut (2016)

<h3>Introduction</h3> Little is known about how undergraduate gastroenterology teaching is delivered in the UK. Our project aims to inform how the BSG could assist in this and to build links with students with an interest in gastroenterology. <h3>Methods</h3> A survey containing 27 questions related to undergraduate gastroenterology training was compiled on surveymonkey.com. We emailed the UK medical school deans and local gastroenterology champions asking them to send out the survey to their medical students. In 10 weeks, 110 students from 12 medical schools responded. <h3>Results</h3> 83% felt that there was adequate exposure to gastroenterology. 75% felt that their training made them confident in managing gastroenterology patients. 9% had a student gastroenterology society (41% don’t have one, 50% not sure). They usually interact via email or Facebook. 59% would consider a career in gastroenterology (53% interested in subject, 24% inspirational consultants, 24% clinic experience, 10% endoscopy experience). 41% would not consider a career in gastroenterology (49% mind set on another specialty, 14% too much endoscopy, 14% out of hours requirement, 7% don’t want to be medical registrar, 6% don’t want to do CMT training, 3% complexity of patients, 3% low financial reward compared to other specialties). Year group sizes ranged from 40–400. 71% had an integrated curriculum whilst 29% had traditional. 20-100% would have a gastroenterology block in their training. Gastroenterology blocks lasted from 1–12 weeks usually in 3<sup>rd</sup> year. Teaching consisted of lectures (99%), small group work (65%), PBL (63%), bedside teaching (65%) and others (13%&gt; GP, home visit, prosection). 65% had gastroenterology and surgical training integrated. 72% said there was a copy of the Gastroenterology syllabus/set of learning objectives within the MBBS curriculum and 60% had read them. Medical final exams involved SBA (83%), short answers (36%), MCQs (65%) and OSCEs (26%). Suggestions for improving gastroenterology training: More consultant training Expert patients as a learning tool Online teaching tools Simulation days Mandatory gastroenterology block More hepatology <h3>Conclusion</h3> There appears to be adequate exposure to gastroenterology training in UK medical schools. Training seems to enable medical students to feel comfortable managing gastroenterology patients in their foundation years. However, there remains scope for enhancing gastroenterology training with students wanting longer dedicated attachments to gastroenterology firms and BSG to do taster days/revision courses/simulation days. Student gastroenterology societies are rare and BSG can help in promoting these. 60% students would consider a career in gastroenterology with interest in the subject being the overriding reason. BSG should capitalise on this and establish links with medical students early in their training. <h3>Disclosure of Interest</h3> None Declared
Subcutaneous Alemtuzumab in Patients with Refractory/Relapsed B-Cell CLL after a Fludarabine-Based Regimen. An Interim Analysis.
Authors: R. Fernando Bezares, German Stemmelin, Daniel Argentieri, Emilio Lanari, Efrain Guy-Garay, Reinaldo Campestri, Miguel Bartomioli, Juan Jose Garcia, Sergio Giralt, Gustav Milone
Journal: Blood (2006)
Essential Thrombocythemia Patients with CALR Mutations Have Increased Platelet Activation Markers Compared to JAK2V617F Mutated Patients
Authors: Maya Koren-Michowitz, Hagit Hauschner, Yulia Shuly, Meital Nagar, Elena Ribakovsky, Mudi Misgav, Odit Gutwein, Uri Seligsohn, Nurit Rosenberg, Arnon Nagler
Journal: Blood (2014)
FRI0540 Evaluation of Nailfoldvideocapillaroscopy in Central Serous Chorioretinopathy: Table 1.
Authors: M.K. Erol, A. Balkarli, D. Toslak, D. Durmaz, B. Dogan, E. Baskan, S. Altun, M. Bulut, V. Cobankara
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Nailfold videocapillaroscopy (NVC) is a diagnostic tool to evaluate micro-vasculature. Presence of choroidal vasculopathy is apparent in central serous chorioretinopathy (CSCR). <h3>Objectives</h3> In this study, it was aimed to assess capillaroscopic findings of nailfold in patients with CSCR. To best of our knowledge, there is no study assessing NVC findings in CSCR in the literature. <h3>Methods</h3> Sixty-one patients with CSCR who met inclusion criteria and 82 age- and sex-matched healthy controls were included to the study. A videocapillaroscopy device with 200x magnification was used for capillaroscopic assessment. <h3>Results</h3> The mean age was 48.79 ± 11.146 years in the patient group (13 female, 48 male) whereas 49.38 ± 9.02 years in the control group (17 female, 65 male). The age and gender were comparable in the patient and control groups (p=0.727 and p=0.933, respectively). The capillary count was found to be decreased in the patient group compared to control group. No significant correlation was found between capillary count and choroidal thickness (p=0.551; r=-0.081). In the patient group, the frequencies of major capillaroscopic findings including capillary ectasia, aneurysm, micro-hemorrhage, avascular area, tortuosity, neo-formation, bizarre capillary, bushy capillary, meander capillary and extravasation were found to be increased in the patient group. However, no significant correlation was detected between capillaroscopic findings and disease type and presence of attacks. <h3>Conclusions</h3> This is first study in which nailfold capillary assessment was performed in patients with CSCR and we detected major capillaroscopic changes. These findings suggest that CSCR can be systemic microvasculopathy. Further studies are needed to clarify the diagnostic and prognostic value of capillaroscopy in CSCR. <h3>Disclosure of Interest</h3> None declared
Regular Aspirin Use and Risk of Non-Hodgkin Lymphoma: A Prospective Analysis in the Nurses' Health Study
Authors: Elsa Brånvall, Karin E Smedby, Bernard A. Rosner, Edward Giovannucci, Jan-Peter Glossmann, Kimberly A Bertrand, Honglei Chen, Francine Laden, Shumin Zhang, Brenda M. Birmann
Journal: Blood (2014)
AB0496 Occurrence of Vascular Access Thrombosis in Patients with Systemic Lupus Erythematosus on Hemodialysis
Authors: N.O. El-Azizi, M. Abd El-Baky, A.B. Abd El-Aziz, E.S. Atwa
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> In patients with lupus nephritis, the presence of antiphospholipid antibodies (aPL) is considered to be an indication of increased risk of thrombotic microangiopathy, a serious complication of SLE <sup>1</sup>. <h3>Objectives</h3> To assess the occurrence of vascular access thrombosis in SLE patients on regular hemodialysis &amp; its relation to the presence of antiphospholipid antibodies. <h3>Methods</h3> The study included 50 SLE female patients on regular hemodialysis; patients further subdivided into; <i>group A</i> included 34 patients with thrombotic history and/or fetal loss and <i>group B</i> included 16 patients without thrombosis and <i>group C</i> 20 female patients on regular hemodialysis with end stage renal disease caused by diseases other than SLE with age matching as a control group. All recruited from the dialysis units in Ahmed Maher Teaching Hospital, and Ain Shams University Hospital. After informed consent all the study population underwent full history taking, detailed clinical examination, measurement of anti-ds DNA, ANA, anticardiolipin IgG &amp; IgM and lupus anticoagulant in addition to CBC, PT, PTT, Creatinine, ALT &amp; AST and duplex on A-V fistula with measurement of intima media thickness. <h3>Results</h3> Age &amp; dialysis duration matched groups were selected (37.24±8.88, 35.69±10.91 vs 37.15±9.21 yrs &amp; 5±3.06, 5.06±3.04 vs 5.65±3.17 yrs respectively). We found that the level of anticardiolipin IgG &amp; IgM and lupus anticoagulant were highly significantly higher in patients of group A than group B than group C (62.18±8.84 vs 20.74±5.73vs 13.41±5.70 u/ml, 46.96±9.72 vs 9.99±3.85 vs 7.12±2.68 u/ml, 63.26±12.05 vs 38.31±6.33 vs 2±0 respectively) (p&lt;0.001). Also there was a highly significant thrombocytopenia in group A (132.41±15.10 vs 270.56±64.78 vs 264.05±67.80x10<sup>3</sup>/μl) with prolonged PT and PTT (17.14±1.74 vs 13.47±1.55 vs 12.40±2.33 sec. and 45.24±6.32 vs 33.25±4.71 vs 29.55±3.62 sec) than group B &amp; group C (p&lt;0.001). There was a highly significant increase in intima media thickness of A-V fistula in group A than group B &amp; group C (119.94±22.22 vs 51.19±15.58 vs 61.91±34.55 mm) (p&lt;0.001). <h3>Conclusions</h3> SLE patients on regular hemodialysis with positive antiphospholipid antibodies have higher incidence of occurrence of vascular access thrombosis. <h3>References</h3> Cohen D, Koopmans M, Kremer Hovinga IC, et al. Potential for glomerular C4d as an indicator of thrombotic microangiopathy in lupus nephritis. Arthritis Rheum. 2008; 58: 2460–9. <h3>Acknowledgement</h3> We thanks all patients participating with us in this study. <h3>Disclosure of Interest</h3> None declared
The Clinical Features and Prognostic Impact of DNMT3A Gene Mutation in Japanese Patients with De Novo AML
Authors: Takeshi Ryotokuji, Hiroki Yamaguchi, Ikuko Omori, Tuneaki Hirakawa, Satoshi Wakita, Tomoaki Kitano, Yoshio Mitamura, Fumiko Kosaka, Takashi Shimada, Koiti Inokuchi, Kazuo Dan
Journal: Blood (2011)
About 17% of AML with NPM1 mutations Show a Specific Pattern of Chromosome Aberrations but These Cases Do Not Differ Prognostically from AML with NPM1 Mutations Carrying a Normal Karyotype
Authors: Claudia Haferlach, Susanne Schnittger, Tamara Weiss, Wolfgang Kern, Brunangelo Falini, Torsten Haferlach
Journal: Blood (2008)
Sequencing of Sipuleucel-T and Androgen Deprivation Therapy in Men with Hormone-Sensitive Biochemically Recurrent Prostate Cancer: A Phase II Randomized Trial
Authors: Emmanuel S. Antonarakis, Adam S. Kibel, Evan Y. Yu, Lawrence I. Karsh, Aymen Elfiky, Neal D. Shore, Nicholas J. Vogelzang, John M. Corman, Frederick E. Millard, Johnathan C. Maher, Nancy N. Chang, Todd DeVries, Nadeem A. Sheikh, Charles G. Drake
Journal: Clinical Cancer Research (2016)

Purpose: STAND, a randomized, phase II, open-label trial (NCT01431391), assessed sequencing of sipuleucel-T (an autologous cellular immunotherapy) with androgen deprivation therapy (ADT) in biochemically recurrent prostate cancer (BRPC) patients at high risk for metastasis.Experimental Design: Men with BRPC following prostatectomy and/or radiotherapy, a PSA doubling time ≤12 months, and no metastasis were enrolled. Patients were randomized (34/arm) to sipuleucel-T followed by ADT (started 2 weeks after sipuleucel-T completion), or ADT followed by sipuleucel-T (started 12 weeks after ADT initiation); ADT continued for 12 months in both arms. The primary endpoint was PA2024-specific T-cell response [enzyme-linked immunospot (ELISPOT)] over time.Results: PA2024-specific ELISPOT responses over time were similar between groups, except at week 6, where responses were higher with sipuleucel-T→ADT versus ADT→sipuleucel-T (P = 0.013). PA2024-specific T-cell proliferation responses, averaged across time points, were approximately 2-fold higher with sipuleucel-T→ADT versus ADT→sipuleucel-T (P = 0.001). PA2024-specific cellular and humoral responses and prostatic acid phosphatase-specific humoral responses increased significantly versus baseline (P < 0.001) and were maintained for 24 months (both arms). Median time-to-PSA recurrence was similar between arms (21.8 vs. 22.6 months, P = 0.357). Development of a PA2024-specific humoral response correlated with prolonged time-to-PSA progression (HR, 0.22; 95% CI, 0.08-0.67; P = 0.007). Sipuleucel-T with ADT was generally well tolerated.Conclusions: Sipuleucel-T→ADT appears to induce greater antitumor immune responses than the reverse sequence. These results warrant further investigation to determine whether this sequence leads to improved clinical outcomes, as well as the independent contribution of ADT alone in terms of immune activation. Clin Cancer Res; 23(10); 2451-9. ©2016 AACR.
Frequency of Agranulocytosis and Mild Neutropenia During Deferiprone Therapy in Clinical Practice
Authors: Mohsen Saleh Elalfy, Shebl Said Shebl, Mohamed A. Badr, Usama R. Elsafy, Mostafa A. Salama, Youssef Al-Tonbary, Yousryeia Abdel Rahman, Mohamad H. Qari, Ghazi Abdullah Al Damnhouri, Zakaria Al Hawsawi, Yasser Ahmed Wali, M. Akif Yesilipek, Yurdanur Kilinc, Dilek Yazman, Zeynep Karakas, Fernando Tricta
Journal: Blood (2012)
<i>Notes from the Field</i>: Cardiac Dysrhythmias After Loperamide Abuse — New York, 2008–2016
Authors: William Eggleston, Jeanna M Marraffa, Christine M Stork, Maria Mercurio-Zappala, Mark K Su, Rachel S Wightman, Karen R Cummings, Joshua G Schier
Journal: MMWR Morbidity and Mortality Weekly Report (2016)

Loperamide is an over-the-counter antidiarrheal with opioid-receptor agonist properties. Recommended over-the-counter doses (range = 2-8 mg daily) do not produce opioid effects in the central nervous system because of poor oral bioavailability and P-glycoprotein efflux* of the medication (1); recent reports suggest that large doses (50-300 mg) of loperamide produce euphoria, central nervous system depression, and cardiotoxicity (2-4). Abuse of loperamide for its euphoric effect or for self-treatment of opioid withdrawal is increasing (5). Cases of loperamide abuse reported to the Upstate New York Poison Center and New York City Poison Control Center were analyzed for demographic, exposure, clinical, and laboratory characteristics. Cases of intentional loperamide abuse reported to the National Poison Database System (NPDS) also were analyzed for demographic, dose, formulation, and outcome information.
Treatment of Patients with Refractory AML with SU5416 Downregulates VEGF-A, STAT5 and Akt Expression in Leukemic Blasts.
Authors: Sonja Loges, Heike Tinnefeld, Anja Metzner, Manfred Jucker, Martin Butzal, Melanie Bruweleit, Uta Fischer, Elena Draab, Gunter Schuch, Anne M. O’Farrell, Dieter K. Hossfeld, Carsten Bokemeyer, Walter Fiedler
Journal: Blood (2005)
Effective Elimination of CML Progenitor and Stem Cells Through Combination of α-CD123 Antibody-Dependent Cell-Mediated Cytotoxicity and Tyrosine Kinase Inhibitor Treatment
Authors: Eva Nievergall, Deborah L. White, Agnes S.M. Yong, Hayley S. Ramshaw, Samantha J. Busfield, Gino Vairo, Angel F. Lopez, Timothy P. Hughes, Devendra K. Hiwase
Journal: Blood (2012)
CD44-Induced Differentiation of Acute Monoblastic Leukemia Cells Is Mediated by Prolonged Activation of ERK1/2 and by Cytokines.
Authors: Sébastien Bourcier, Arnaud Sansonetti, Laetitia Durand, Christine Chomienne, Jacqueline Robert-Lézènès, Florence Smadja-Joffe Sr., PhD
Journal: Blood (2009)
SAT0217 Axon Reflex Vasodilatation of Digital Arteries in Systemic Sclerosis Patients, Evaluated by Laser-Doppler Fluxmetry
Authors: C. Rotondo, M. Nivuori, A. Chialà, E. Praino, L. Coladonato, M.G. Anelli, M. Giannini, N. Lascaro, R. Fanizzi, G. Laselva, F. Cacciapaglia, G. Lapadula, F. Iannone
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Systemic sclerosis (SSc) is a connective disease characterized by severe microvessels vasculopathy. The important role of endothelial dysfunction in SSc pathogenesis is widely demonstrated, but the SSc related autonomic nervous system impairment is controversial and rarely described. The dysregulation of axon reflex vasodilatation of fingertip microvessels in SSc has been reported in some studies, but the functional involvement of digital arteries in SSc patients had never been investigated. <h3>Objectives</h3> We aimed at evaluating the blood flux changes mediated by axon reflex response upon heating stimulus in SSc patients. <h3>Methods</h3> 87 SSc patients (SSc pt) and 22 healthy controls (HC) were recruited in this study. The SSc patients were aged 52,7±16,2 yrs, with disease duration of 8,23±7,8 yrs. The LD Flowmetry (Periflux System 5000, Perimed) with 4 LD heating probes was used to measure the skin blood flux of the middle phalanx of the 2nd, 3rd, 4th and 5th fingers of the left hand. The skin fingers flux was recorded at pre-heating period with probes temperature fixed at 34 °C (PHF) and after 5 min heating at 44 °C (IHF). Therefore, the heating test assesses the variation of digital artery flux in response to local heating stress at 44 °C. The ratio IHF/PHF evaluates the axon reflex mediated vasodilatation (ARMV). The results are expressed as average across 4 fingers at each time. The flux was expressed in perfusion unit (PU) and HC and SSc patients responses were compared by the t-student test. Statistic significance was set at p≤0,05. The data analysis was performed using IBM SPSS statistic 20. <h3>Results</h3> The ARMV was significantly reduced in SSc patients (2,2±1,4) in comparison with HC (4,8±2,7, p=0,0001). IHF was lower in SSc patients (118,6±54,3) than HC (168±78, p=0,001). Dividing the SSc pts group by the presence of pitting scars (PS), digital ulcers (DU) and digital necrosis (DN), we observed a lower IHF in SSc pts with PS (103,2±48,6) than in SSc pts without (138,1±56, p=0,003), and in SSc pts with DU (97,8±47,6) than in SSc pts without (160,27±120,1, p=0,002). Moreover, SSc pts with ND showed a lower ARMV (1,8±1) than SSc pts without (2,6±1,5, p=0,006), and a lower IHF (98,3±49,1 vs 133,2±54,5, p=0,004). The age, sex, concomitant therapy with Ca-antagonist or Bosentan or cardioaspirin did not influence the digital artery flux. <h3>Conclusions</h3> This study provided evidence that a functional impairment of DA occurs in SSc, and it worsens with increasing severity of vascular clinical manifestations. <h3>Disclosure of Interest</h3> None declared
Hydroxyurea Induces Fetal Hemoglobin Expression by Activating cAMP Signaling Pathways In a cAMP- and cGMP-Dependent Manner; New Hypothesis to Account for a Role of Non-Erythroid Cells In Fetal Hemoglobin Induction
Authors: Tohru Ikuta, Diana Gutsaeva, James Parkerson, Shobha Yerigenahally, C. Alvin Head
Journal: Blood (2010)