tbuckley/cabot-search huggingface dataset:
Abstract 3310: Ezrin is an independent prognostic factor in extremity soft tissue sarcomas
Authors: Ana Carneiro, Mats Jönsson, Eva Rambech, Per Rissler, Anders Rydholm, Mef Nilbert
Journal: Cancer Research (2010)

Abstract Ezrin is a cytoskeleton protein actively involved in regulating the growth and metastatic capacity of cancer cells. It has recently been reported to be to be associated with poor prognosis high grade soft tissue sarcoma (STS) and to mediate growth and survival through AKT pathway. To further validate the prognostic value of ezrin in STS, we evaluated 229 extremity primary STS using tissue microarrays and immunohistochemistry. Ezrin expression was correlated with metastasis during follow-up for at least 5 years or until death.185 of the 229 cases analyzed (80%) showed ezrin immunoreactivity in the membrane and cytoplasm of the tumor cells. Multivariate analysis with other known risk factors, including size, vascular invasion and necrosis showed that ezrin expression was significantly associated with development of distant metastasis during follow-up (P = 0.01, 95%CI 1.4-3.4). Ezrin expression was also correlated with AKT pathway changes, evaluated by gene expression. Our findings suggest that ezrin is a promissing prognostic marker in highly malignant STS and support an interaction between ezrin and AKT in the genesis of STS metastasis. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3310.
Abstract 1611: Identification of target molecules and anti-leukemic effects of novel phospha sugar derivative, TMPP
Authors: Satoki Nakamura, Tomonari Takemura, Daisuke Yokota, Isao HIrano, Takaaki Ono, Kazuyuki Shigeno, Kazunori Ohnishi
Journal: Cancer Research (2010)

Abstract [Background and Aims] We synthesized two phospha sugar derivatives, 2,3,4-tribromo-3-methyl-1-phenylphospholane 1-oxide (TMPP) by reacting 3-methyl-1-phenyl-2-phospholene 1-oxide with bromine, and we has reported their potential as an antileukemic agent in cell lines. In this study, we have investigated that TMPP have antileukemic effects as shown in various leukemia cell lines as well as primary AML patient specimens. Moreover, we have identified the target molecules of TMPP by using docking simulation. [Methods] In human leukemia cell lines (HL60, NB4, U937, NOMO-1, CEM, MOLT4, SUP-B15, and YRK2 cells), the anti-leukemic effects were elevated by cell proliferation assay, Cell cycle analysis, Western blotting. In AML cells derived from AML patients and normal hematopoietic progenitor cells derived from healthy volunteers, the changes of viability by treatment with TMPP were evaluated. By using docking simulation, the target molecules were identified in leukemia cells treated with TMPP. [Results] TMPP showed inhibitory effects on leukemia cell proliferation, with mean IC50 values of 10.2 nmol/L for TMPP, indicating that inhibition appeared to be dependent on the number of bromine atoms in the structure. Further, TMPP at 8.4 nmol/L induced G2/M cell cycle block in leukemia cells, and TMPP at 17.3 nmol/L induced apoptosis in these cells. TMPP treatment reduced cell cycle progression signals (FoxM1, KIS, Cdc25B, Cyclin D1, Cyclin A, and Aurora-B) and tumor cell survival (Bcl-2, p27Kip1 and p21Cip1). Further, treatment with TMPP significantly reduced the viability of AML specimens derived from AML patients, but only slightly reduced the viability of normal ALDHhi progenitor cells. We also found that TMPP showed the strong activity against Aurora kinase B and Bcl-2 by using docking simulation. [Conclusion] We demonstrate that TMPP inhibits Aurora kinase B and Bcl-2 and shows a dominant Aurora B kinase inhibition-related cell cycle arrest and the induction of mitochondrial potential catastrophe-related apoptosis in acute leukemia cells. Moreover, we identified Aurora kinase B and Bcl-2 as the target molecules of TMPP. We conclude that TMPP has great therapeutic potential in anticancer therapy in a wide range of cancers. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1611.
Successful re-intervention with metal stent trimming using argon plasma coagulation after endoscopic ultrasound-guided hepaticogastrostomy
Authors: Kei Yane, Akio Katanuma, Hiroyuki Maguchi, Kuniyuki Takahashi, Manabu Osanai, Toshifumi Kin, Satoshi Ikarashi, Ryuki Minami, Manabu Sen-yo, Itsuki Sano, Hajime Yamazaki
Journal: Endoscopy (2014)

Recently, endoscopic ultrasound (EUS)-guided biliary drainage has been introduced as an alternative method after failed endoscopic biliary drainage, particularly in patients with a pre-existing duodenal obstruction [1] [2] [3]. A longer self-expandable metal stent (SEMS) is usually used for EUS-guided hepaticogastrostomy (EUS-HGS) to prevent stent migration. However, re-intervention after EUS-HGS is challenging because of the protrusion of the SEMS into the stomach. Metal stent trimming using argon plasma coagulation (APC) has been reported to be a useful option for stent-related complications such as dislocation [4] [5]. We report a case in which successful re-intervention after EUS-HGS was made possible by metal stent trimming using APC.
Commentary: Biomarkers in Epidemiology: Scientific Issues and Ethical Implications
Authors: Paul A. Schulte
Journal: Environmental Health Perspectives (1992)

The current generation of biologic markers have three characteristics that differentiate them from previous ones. These include the ability to detect xenobiotics at concentrations at the cellular and molecular level, to detect earlier biologic changes presumptive of disease or disease risk, and to identify a detailed continuum of events between an exposure and resultant disease. If biomarkers are to enhance cancer epidemiology, they must be valid, reliable, and practical. When these characteristics have not been previously demonstrated, pilot studies should be conducted prior to the primary study. Interdisciplinary communication and collaboration is required so that useful markers are selected and that collection and handling, assay, and interpretation are appropriate. The status of many biomarkers is that they have been developed in the laboratory but lack validation for field use. Validation of a marker for use in a population requires attention to issues of background prevalence, sample size, natural history, persistence, variability, confounding factors, and predictive value. Additionally, practical features such as subject preparation, access to specimens, specimen storage aspects, and costs must be clarified. Ultimately, the use of biologic markers in epidemiologic studies will depend on how well the markers increase ability to reduce misclassification, provide for better interpretation of exposure-disease associations, and increase opportunities for prevention. Validation studies and general research using biomarkers also have clinical, ethical, and legal implications. These range from communicating uncertainty about the meaning of a marker to the kinds of societal response that result when groups or individuals are identified as having an abnormal marker frequency.
Retrograde Vertebral-Artery Flow Produced by Correction of Subclavian-Steal Syndrome
Authors: Robert L. Berger, James J. Sidd, K. Ramaswamy
Journal: New England Journal of Medicine (1967)

DESCRIBED originally by Contorni1 in 1960 and christened by the editors of this journal,2 the subclavian-steal syndrome has since become a well established clinical entity.3 , 4 It is associated with occlusion of the subclavian artery proximal to the origin of the vertebral. The syndrome is characterized by diversion of blood from the opposite vertebral artery into the obstructed side, with resultant perfusion of the distal subclavian bed with blood that was intended for cerebral circulation. The symptoms are reminiscent of basilar-artery insufficiency and may be precipitated by exertion of the involved extremity.5 The occlusion is usually caused by degenerative vascular disease, . . .
EVALUATION OF CLINICAL FEATURES IN PATIENTS WITH DECREASING ANKLE-BRACHIAL VALUE: A SUBANALYSIS OF THE IMPACT-ABI REGISTRY
Authors: Naoto Hashizume, Takashi Miura, Yasushi Ueki, Tomoaki Mochidome, Mikiko Harada, Naoyuki Abe, Hitoshi Nishimura, Soichiro Ebisawa, Hirohiko Motoki, Ayako Okada, Yuji Shiba, Atsushi Izawa, Jun Koyama, Uichi Ikeda
Journal: Journal of the American College of Cardiology (2016)
AB0342 Contribution of high resolution ultrasound in the early diagnosis of rheumatoid arthritis (RA) with negative immunological tests
Authors: W. Hamdi, M. Bouaziz, M.M. Ghannouchi, F.M. Ladeb, M.M. Kchir
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The early diagnosis of RA is often difficult in the absence of immunologic markers, bone erosions on radiographs or clinical characteristic features such as typical joint involvement or rheumatoid nodules. <h3>Objectives</h3> The aim of this study was to assess the contribution of High resolution Doppler Ultrasound in the early diagnosis of RA with negative immunologic markers. <h3>Methods</h3> Cross sectional study of 44 patients admitted in our institution between 2009 and 2011. Inclusion criteria were the following: recent onset inflammatory rheumatism (&lt;2 years), negative immunological markers, absence of bone erosion or joint narrowing on radiographs of both hands. All patients had High resolution Doppler ultrasound of both hands using a 15 Mhz linear probe to assess synovitis, tenosynovitis, bone erosion and Doppler hyperhemia. <h3>Results</h3> Thirty four women and ten men fulfilled the inclusion criteria. The mean age was 53±13 [18-76] years. The onset of symptoms was mono-articular in 54.5%, oligo-articular in 11.3% and polyarticular in 34.2% of cases. The mean duration of the disease was 11.2±7.6 [2-24] months. Radiographs showed epiphyseal bone demineralization in 12 cases. Ultrasound showed at least one synovitis in 39 patients, 17 of which associated with Doppler hyperhemia. More than 5 joints were involved in 32 patients and bone erosions were detected in 19 cases. High resolution ultrasound allowed confirmation of early RA in 43.1% of cases since it showed bone erosions in 19 patients with inflammatory rheumatism, elsewhere ultrasound has helped the diagnosis of early inflammatory rheumatism since it showed polysynovitis in 14 of 24 patients showing a mono-articular clinical involvement. <h3>Conclusions</h3> High resolution Doppler ultrasound is more sensitive than Radiographs in detecting bone erosions, synovitis and hyperhemia. Combined with a meticulous clinical examination, US is useful in the early diagnosis of RA. <h3>Disclosure of Interest</h3> None Declared
P1091 MONITORING THE IMMUNOMODULATORY EFFECTS OF PEGYLATED INTERFERON α-2a THERAPY IN PATIENTS WITH CHRONIC HBV INFECTION, AN IMMUNOLOGICAL SUB-STUDY OF ANRS HB06 PEGAN TRIAL
Authors: C. Aspord, J. Bruder-Costa, T. Dufeu-Duchesne, S. Larrat, J.-P. Zarski, J. Plumas, V. Leroy
Journal: Journal of Hepatology (2014)
HETEROGENEITY IN EFFICACY OF ROLOFYLLINE USING A RISK-BASED EVALUATION IN PATIENTS HOSPITALIZED WITH ACUTE HEART FAILURE
Authors: Biniyam Demissei, Douwe Postmus, Licette Liu, John Cleland, Christopher M. O’Connor, Marco Metra, Piotr Ponikowski, John Teerlink, Gad Cotter, Beth Davison, Michael Givertz, Daniel Bloomfield, Howard Dittrich, Adriaan Voors, Hans Hillege
Journal: Journal of the American College of Cardiology (2016)
AB1391 The validity of duruÖz hand index (DHI) in geriatric population
Authors: T. Duruöz, E. Topcu, E. Duruöz, U. Ucar
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The Duruöz Hand Index (DHI) [1] has 18 items of daily activities questions. It was developed to assess the functional disability of rheumatoid hand and cross validated for some other arthropaties of the hand (osteoarthritis, systemic sclerosis, carpal tunnel syndrome, stroke, flexor tendon rupture etc.). <h3>Objectives</h3> To assess the usefulness of the DHI to evaluate functional disability of hands in geriatri population. <h3>Methods</h3> Subjects aged more than 65 were recruited into the study. The local or systemic diseases which may affect directly the hand function were exclusion criteria. Demographic and functional characteristics of subjects were evaluated. The functional assessment was performed with DHI, Hand Functional Index (HFI), Health Assessment Questionnaire (HAQ), Purdue Pegboard (fingertip dexterity and hands coordination) and grip strength (JAMAR) and 3 kinds of pinch (tip, lateral, chuck) strengths (JAMAR). The DHI was correlated (Spearman's correlation coefficient) with the other functional parameters to assess the convergent validity and with non-functional parameters to assess the divergent validity. p&lt;0.05 was significant. <h3>Results</h3> Fifty patients (33 female) with mean age 72.6 (range: 65-90) were recruited. The average score of DHI was 16.32 (SD: 17.57) and 26.0% of patients had zero score (without disability). The DHI had not any significant relation with demographic data such as age, hand dominancy, alcohol consumption, smoking and DHI has poor or fair correlation with fatigue level and Pittsburg Sleep Quality Index (divergent validity). The DHI has significant and good correlation with Health Assessment Questionnaire (HAQ), Hand Functional Index, Purdue Pegboard scores, grip strength and 3 types of pinch strengths (convergent validity). The strongest relation of DHI is with HAQ (rho: 0.782; p&lt;0.0001). <h3>Conclusions</h3> The DHI is practical scale and it is useful to assess the functional disability of hands accurately in geriatric population. <h3>References</h3> Duruöz MT et al. Development and validation of a rheumatoid hand functional disability scale that assess functional handicap. J Rheumatol 1996; 23:1167-72. <h3>Disclosure of Interest</h3> None Declared
Abstract 193: Dissecting satellite and imprinting genes methylation status in cloned bovine: Aberrant methylation in imprinting loci
Authors: Chih-Jie Shen, Chiao-Chieh Ling, Perng-Chih Shen, Winston T. K. Cheng, Hsiao-Ling Chen, Chuan-Mu Chen
Journal: Cancer Research (2010)

Abstract In mammals, genome-wide epigenetic reprogramming systems exist in primordial germ cells and zygote. It plays a crucial role in regulating genome functions at critical stages of embryo development and confers stability of gene expression during mammalian development. Inappropriate reprogramming of donor nuclei in somatic cell resulted in neonatal death and preimplantation defects during animal cloning technique. Specific demethylation events in differentiated tissues could then lead to further changes in gene expression as needed. In this study, six cloned bovines created by ear fibroblast nuclear transfer with short life span and multiple organ defects were used as the experimental materials. We focus on three imprinting genes included two growth factor genes IGF-2, H19 and Xist (X chromosome inactive regulated gene) by combined bisulfite and restriction assay (COBRA). Bisulfite sequencing was also applied to analyze the aberrant CpG sites in different imprinted genes loci in these cloned bovine genomes. Our data show that loss of imprinting (LOI) phenomenon was frequently appeared in IGF-2, Xist and H19 imprinted loci in several tissues of these cloned bovines. Furthermore, we detected the cloned bovine NTG-2 expression of these imprinting genes with quantitative real time PCR (Q-PCR). The data suggested that IGF-2 and H19 are extremely overexpressed in liver, vein, ear, skin and uterus. The IGF-2 mRNA expression in uterus and liver of NTG-2 cow are almost 1,200 and 800 folds compared to WT cow. The H19 expression level in liver of NTG-2 also exhibited a 34,000-fold higher than that of WT cow. According to our research, the extensively overexpression of IGF-2 and H19, probably were the major reason to cause the cloned bovine NTG-2 not only organ defects, but also aberrant embryonic development. In conclusion, the death of clones may be due to aberrant DNA methylation at the loci of imprinting genes and disruption of incompletely reprogrammed after nuclear transfer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 193.
Abstract 1517: The outcome of inhibition of stromal TGFβRII in pancreatic cancer is tumor cell-dependent
Authors: Katherine T. Ostapoff, Bercin K. Cenik, Kyla Carroll, Rolf A. Brekken
Journal: Cancer Research (2012)

Abstract An elevated level of TGF-β is a negative prognostic indicator for patients with pancreatic ductal adenocarcinoma (PDAC). Despite this, the function of TGF-β signaling in tumor microenvironment stromal cells is unclear. We have studied how TGF-β-induced signaling in stromal cells (macrophages and fibroblasts) impacts human pancreatic cancer cell lines (Capan-1, MiaPaca-2, C5LM2 and Colo357) in vitro and in vivo. The TGFβRII activity in Raw 264.7 macrophages and 3T3 fibroblasts was inhibited with 2G8, a monoclonal rat anti-mouse TGFβRII IgG. Raw cells treated with 2G8 adopt a predominantly pro-inflammatory phenotype in vitro as documented by increased Hes1 and MCP-1 expression and inhibited tumor cell migration. Colony formation in soft agar was inhibited by conditioned media (CM) of Raw cells treated with 2G8 in all lines tested, except MiaPaca-2. Raw cell CM inhibited tumor cell proliferation in vitro compared to control media-treated tumor cells, but there were no significant differences between CM from Raw cells treated with 2G8 vs. a control IgG. CM from 3T3 cells treated with 2G8 also inhibited tumor cell migration and soft agar colony formation while control CM from 3T3 stimulated soft colony formation. MiaPaca-2, Capan-1 and Colo357 cells were orthotopically injected in SCID mice and were treated with saline or 2G8. 2G8 therapy differentially affected primary tumor size, such that inhibition of stromal TGFβRII stimulated the growth of MiaPaCa-2 tumors but inhibited the growth of Capan-1 and Colo357 primary tumors compared to treatment with a control IgG. Interestingly, 2G8 which only blocks mouse TGFβRII, suppressed metastasis in mice bearing MiaPaca-2, Capan-1 and Colo357 tumors. Furthermore, in isogenic cell lines derived from a 5 week old PDAC transgenic (p48-Cre; KrasG12D; Ink4alox/lox) mouse we also found a differential response to 2G8 therapy. Subcutaneous tumors that arose from clone MPLR 2D, were repressed by 2G8 therapy while subcutaneous tumors that arose from clone MPLR3B were stimulated by 2G8 therapy. The nature of differential response of the primary tumors and the mechanism of suppression of metastasis is under vigorous investigation. Our data thus far underscore the complexity and challenge of targeting the TGF-β pathway in pancreatic cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1517. doi:1538-7445.AM2012-1517
Medication Monitors to Treat Tuberculosis
Authors: THOMAS S. MOULDING
Journal: American Journal of Respiratory and Critical Care Medicine (1999)

The use of directly observed therapy (DOT) for nearly all cases of pulmonary tuberculosis (TB) is being widely promoted by the Centers for Disease Control, but its implementation is being resisted by many health professionals. Consequently, less than half of the patients in major metropolitan health departments were given DOT in 1996. The usual justification for “universal” DOT instead of selective DOT is the well-known difficulty in differentiating between patients who are reliable in taking medication from those who are not. Devices called medication monitors, which record when medication is removed from a container, were shown to be effective in determining the reliability of TB patients in taking medication in the 1960s but were cumbersome to use. Since then several improved, convenient to use, electronic medication monitors have been introduced and further improvements can be anticipated. These increasingly practical medication monitors need to be studied as a supplement to DOT in order to make selective DOT an effective alternative to “universal” DOT in managing the medication compliance problem when treating TB.
248 FASTING AMMONIA (NH3) AS A PREDICTOR OF HEPATIC ENCEPHALOPATHY (HE) EVENTS
Authors: J.M. Vierling, M. Mokhtarani, R.S. Brown, P. Mantry, D.C. Rockey, M. Ghabril, D.F. Coakley, D. Millikien, B.F. Scharschmidt
Journal: Journal of Hepatology (2013)
Palisaded Neutrophilic and Granulomatous Dermatitis
Authors: Meena Singh, Nneka Comfere
Journal: New England Journal of Medicine (2012)

A 26-year-old man presented with a 3-year history of recurrent crops of expanding annular and gyrate plaques on his head, neck, torso, and proximal extremities. Systemic symptoms included low-grade fever, polyarthralgia, chronic cough, weight loss, and abdominal pain.
Automatic detection of over 100 anatomical landmarks in medical CT images: A framework with independent detectors and combinatorial optimization
Authors: Shouhei Hanaoka, Akinobu Shimizu, Mitsutaka Nemoto, Yukihiro Nomura, Soichiro Miki, Takeharu Yoshikawa, Naoto Hayashi, Kuni Ohtomo, Yoshitaka Masutani
Journal: Medical Image Analysis (2016)