tbuckley/cabot-search huggingface dataset:
Abstract 4263: Dynamic phenotype switching regulated by epigenetic marks along melanoma genesis
Authors: Alice S. Morais, Fernanda Molognoni, Miriam Galvonas Jasiulionis
Journal: Cancer Research (2012)

Abstract Melanoma is the most aggressive form of skin cancer and its incidence is increasing worldwide. Although melanoma is highly curable when early diagnosed, the metastatic melanoma detains the worst prognosis and remains one of the most resistant cancers to treatment. In this light, continuous efforts are made to better understand the pathogenesis of this disease. It has been strongly suggested that aberrant epigenetic events, occurred in specific steps, are important to the establishment and progression of tumor genesis as the occurrence of point genetic mutations. Based on a murine model of malignant transformation, the aim of our group has been identify and understand the effective participation of epigenetic mechanisms in the generation of a transformed phenotype. The study model of melanoma genesis was established in vitro by submitting non-tumorigenic melanocytes (murine melan-a cell line) to sequential cycles of anchorage blockade, proposing that microenvironment interference may lead to intracellular alterations with important gene expression impact. Our cell lines represent distinct phases of melanoma genesis (melan-a melanocytes, 4C pre-malignant melanocytes, 4C11- non-metastatic and 4C11+ metastatic melanoma) and, on an overview, it was observed clearly morphological differences, mainly in intermediate stages of progression, suggesting a transient change from epithelial to mesenchymal phenotype (from melan-a to 4C and 4C11- cell lines) along malignant transformation. This transitory modification was accompanied by a switch in the EMT markers expression, like Snail1 and your targets, β-catenin, E-cadherin and N-cadherin. In parallel, Chd1 (chromatin remodeling factor), Nanog (pluripotency factor) and Jarid1b (histone demethylase) are highly expressed in these intermediate phases (4C and 4C11-), characterizing an open chromatin configuration. Interestingly, expression of most of these genes appear to be controlled by epigenetic mechanisms, both DNA methylation and chromatin modification. We hypothesized that during melanocyte malignant progression associated with sustained stress, an opened chromatin occurrence would facilitate cells to undergo changes in gene expression which allow cell adaptation to new environment and all these alterations are orchestrated by epigenetic events. Supported by FAPESP. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4263. doi:1538-7445.AM2012-4263
P2-10-10: A Precision Comparison of Breast Ultrasound Images between Different Time Phases by Imaging Fusion Technique Using Magnetic Position Tracking System.
Authors: S Nakano, K Fujii, K Yorozuya, M Yoshida, T Fukutomi, O Arai, T Mitake
Journal: Cancer Research (2011)

Abstract Purpose: We developed a real-time virtual sonography (RVS) in which realtime ultrasound (US) images and reconstructive virtual US images of the same cross-section are synchronized based on US volume data obtained on the same monitor using magnetic position tracking system (US-RVS). The purpose of this study was to evaluate the accuracy of US-RVS to compare breast US images between different time phases. Materials and Methods: Between March 2010 and April 2011, US-RVS was performed in 108 consecutive patients with 112 target lesions for US (mean lesion size: 12 mm, 54 malignant and 58 benign). We used US-RVS system which consisted of sonography equipment, magnetic field generator, magnetic sensor, and workstation. To assess the accuracy of the US-RVS, we analyzed the sonographic re-identification rate of target lesions in different time phases. Results: Among the 112 target lesions, 105 (94%) lesions were re-identified by US-RVS. The remaining occult 7 (6%) lesions were included one DCIS and 6 fibrocystic changes in terms of the histological type, and one mass lesion and 6 nonmass like lesions in terms of morphological feature. US-RVS enabled us to precisely compare arbitrary cross-sections of US images in any direction between different time phases independent of the operator's skill. Conclusion: US-RVS, which requires no contrast medium and involves no radiation exposure, can be a useful modality for temporally tracking regions of interest using US. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P2-10-10.
Endoscopic and ultrasonic endoscopic features of gastrointestinal carcinoid
Authors: Qingxiang Yu, Yixiang Chang, Bangmao Wang, Wentian Liu
Journal: Endoscopy (2011)

Carcinoid tumors are slow-growing and usually become symptomatic late in the course of the disease. We evaluated our experience in the management of GI carcinoid tumors to investigate the endoscopic and ultrasonic endoscopic features of GI carcinoid.
Abstract 2057: Hypoxia stimulates the epithelial-mesenchymal transition of gastric cancer cells via TGF-β/ TGF-β receptor signaling
Authors: Junko Matsuoka, Masakazu Y, Shinichiro Kashiwagi, Yukihiro Kato, Yuhiko Fuyuhiro, Naoshi Kubo, Hiroaki Tanaka, Kazuya Muguruma, Nobuya Yamada, Kiyoshi Maeda, Masaichi Ohira, Tetsuro Ishikawa, Kosei Hirakawa
Journal: Cancer Research (2011)

Abstract Introduction: Epithelial mesenchymal transition (EMT) is a cellular process during which epithelial cells acquire mesenchymal properties while losing cell-cell interactions and apicobasal polarity. EMT is considered to be correlated with malignancy of cancer cells and to be responsible for cancer invasion and metastasis. Patients with gastric cancer show a poor prognosis because of frequent metastasis. We previously reported that hypoxia was associated with distant metastasis and poor prognosis in gastric cancer. Therefore, we investigated the effect of hypoxic condition on EMT of gastric cancer cells. Materials and Methods:Three gastric cancer cell lines, OCUM-2MD3, OCUM-2M, and OCUM-12 derived from gastric cancer were used. Cells were cultured in normoxia (21% O2) or hypoxia (1% O2). EMT rate was evaluated as the percentage of spindle-shaped cells of total cells. The expression level of TGF-β and TGF-β receptor was evaluated by real time RT-PCR. Effect of TGF-β or TGF-βR inhibitor, SB431542, on the EMT was evaluated by cell morphology. Results: Hypoxia stimulated EMT of OCUM-2MD3 and OCUM-12 cells, but not that of OCUM-2M cells. The level of TGF-β expression was increased significantly (p&amp;lt;0.001) under hypoxia in comparison with normoxia in all of three cell lines. The level of TGF-β receptor expression was increased in OCUM-2MD3 and OCUM-12 cells, but not OCUM-2M cells. TGF-βR inhibitor, SB431542, significantly suppressed EMT of OCUM-2MD3 (p=0.03) and OCUM-12 (p&amp;lt;0.01). Conclusion: Hypoxia stimulates the EMT of gastric cancer cells via TGF-β/ TGF-β receptor signaling. TGF-β receptor inhibitor might be promising for the treatment of gastric cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2057. doi:10.1158/1538-7445.AM2011-2057
A1.34 Oncostatin M differentially regulates TNFα-induced pro-inflammatory mechanisms in the RA joint
Authors: M Hanlon, D Veale, S Cregan, M Biniecka, U Fearon, T McGarry
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Introduction</h3> Oncostatin M (OSM) is a pleiotropic cytokine that has both agonistic and antagonistic effects depending on the inflammatory microenvironment. This study examines the effect of OSM on TNFα-induced pro-inflammatory mechanisms and on Notch-1 signalling in Rheumatoid Arthritis. <h3>Methods</h3> Primary RA synovial fibroblasts (RASFC) isolated from RA synovial biopsiesobtained at time of knee arthroscopy and human dermal microvascular endothelial cells (HMVEC) were grown to confluence. RASFC and HMVEC were cultured with OSM (10ng/ml) alone or in combination with increasing concentrations of TNFα (0.01 – 1ng/ml). IL-6, IL-8, RANTES, GROα and MCP-1 cytokines/chemokines were quantified in culture supernatants by ELISA. Functionally, angiogenesis and RASFC invasion were assessed by matrigel tube formation and Transwell invasions assays, VEGF in cell lysates by Real-time PCR. Finally, Notch-1, its ligands Delta-like-ligand 4 (DLL4)and Jagged-1 (Jag-1) and downstream transcriptional repressors-Hey-1 and Hey-2 were quantified by Real-time PCR. <h3>Results</h3> OSM alone significantly induced IL-6 and MCP-1 while inhibiting IL-8 and GROα in RASFC and HMVEC culture supernatants. OSM alone induced RANTES expression in HMVEC with no effect observed for RASFC. OSM potentiated the effect of TNFα on IL-6 and MCP-1 secretion from RASFC and HMVEC. Conversely OSM inhibited TNFα-induced-IL-8 and GROα secretion from RASFC and HMVEC. Interestingly, OSM significantly inhibited TNFa-induced RANTES expression in HMVEC and conversely potentiated this effect in RASFC. At a functional level, OSM induced RASFC and HMVEC invasion and network formation and induced VEGF expression compared to basal control. Finally OSM significantly induced Notch-1 and Hey-2 in RASFC and HMVEC, but interestingly differentially regulated the Notch-1 ligands, with induction of Jag-1 only observed in RASFC, and induction of DLL-4 only observed in HMVEC. <h3>Conclusion</h3> OSM is a pleiotrophic cytokine that differentiallyregulates pro-inflammatory mechanisms within the inflamed joint, effects that appear to be dependent on cell type and the inflammatory microenvironment. Targeting OSM or downstream signalling pathways may have therapeutic potential.
Closing the Loop: Practical and Theoretical
Authors: Charles M Peterson, Lois B Jovanovic, Michael Brownlee, Robert L Jones, Anthony Cerami
Journal: Diabetes Care (1980)

Using presently available technologies, a closed-loop system can be devised that employs patient-monitored glucose determinations and self-adjusted insulin administration. Improved glucose control has been documented in type I diabetes and in pregnant women with insulin-dependent diabetes mellitus. In pregnancy, self-administered insulin may achieve levels of carbohydrate control that are superior to those achieved using a self-contained glucose-controlled insulin infusion system, programmed by existing algorithms. Improved closed-loop systems are necessary in order to achieve carbohydrate “control” that will surpass that which can be attained using presently available means. A potential approach might employ a lectin glucose-controlled insulin delivery system,
HISTOLOGICAL STUDIES ON HOG CHOLERA
Authors: O. Seifried, C. B. Cain
Journal: The Journal of Experimental Medicine (1932)

1. The hemorrhages, foci of necrosis, and anemic infarcts met with in the various organs in virus hog cholera, result primarily from the vascular lesions described in a previous paper of this series. Although they are not dependent on the presence of secondarily invading bacteria, their severity is influenced by these organisms. 2. The lesions in the lymph nodes, spleen, kidneys, and central nervous system seem to be of special diagnostic value in questionable cases of hog cholera. The presence of an encephalitis alone does not justify the diagnosis of hog cholera because in the central nervous system of swine similar inflammatory lesions occur in other diseases.
CLINICAL EPIDEMIOLOGY OF CHYLOTHORAX AFTER CARDIAC SURGERY IN CHILDREN: A REPORT FROM THE PEDIATRIC CARDIAC CRITICAL CARE CONSORTIUM
Authors: Jason Buckley, Eric Graham, Michael Gaies, Jeffrey A. Alten, David Cooper, John Costello, Yuliya Domnina, Janet Donahue, Darren Klugman, Sara Pasquali, Wenying Zhang, Mark Scheurer
Journal: Journal of the American College of Cardiology (2016)
EFFECTS OF AEROBIC EXERCISE ON VASCULAR FUNCTION AND INFLAMMATION
Authors: Evangelos Oikonomou, Dimitrios Athanasiou, Gerasimos Siasos, Konstantinos Mourouzis, Gerasimos Terzis, Aggeliki Stasinaki, Efstathios Dimitropoulos, Nikolaos Gouliopoulos, Eleni Kokkou, Panagiotis Tourikis, Konstantinos Spengos, Dimitris Tousoulis
Journal: Journal of the American College of Cardiology (2016)
Abstract 3973: MiR-21 modulates hTERT through a STAT3-dependent manner on glioblastoma cell growth
Authors: Guan Sun, Yingyi Wang, Junxia Zhang, Xiefeng Wang, Lihua Sun, Peiyu Pu, Chunsheng Kang, Ning Liu, Zhen Fu, Yongping You
Journal: Cancer Research (2011)

Abstract MiRNAs, are small noncoding RNA molecules of approximately 20-22 nucleotides in length, regulate protein expression by cleaving or repressing the translation of targets mRNA. MiR-21, as an oncogene, has been reported to be dyregulated in a variety of cancer. Our recent data have verified that miR-21 is one of the most frequently over-expressed miRNA in human glioblastoma (GBM) cell lines. However, the mechanism of miR-21 involved in gliomagenesis is still unknown. In this study, reduction of miR-21 by antisense oligonucleotide induced cell apoptosis and inhibited cell growth in U87 and LN229 GBM cells, with a decreased expression of human telomerase reverse transcriptase (hTERT). Knockdown of hTERT expression using lentiviral vector mediated delivery of hTERT-siRNA triggered cell apoptosis and cell cycle G1/G0 arrest in U87 and LN229 cells. In order to explore the molecular regulation of hTERT by miR-21, we found that STAT3 could be an important mediator between miR-21 and hTERT. Reduction of miR-21 repressed STAT3 expression and STAT3 phosphorylation. STAT3 inhibitor WP1066 led to a remarkable depletion of hTERT both at mRNA and protein levels. To determine the functional interaction between STAT3 and hTERT, ChIP showed the presence of STAT3-binding sites in the hTERT gene. Luciferase reporter assay revealed that the hTERT gene promoter was direct bound by STAT3. Furthermore, we evaluated the status of STAT3 in hTERT regulation by miR-21. U87 and LN229 cells that treated with miR-21 antisense oligonucleotide and IL-6 which could elevate the expression of STAT3 showed a significant increase of hTERT expression, whereas cells that treated with miR-21 oligonucleotide and STAT3 inhibitor WP1066 displayed a reduction of hTERT expression. Finally, knockdown of miR-21 considerably inhibited tumor growth and diminished the expression of STAT3, pSTAT3 and hTERT in xenograft model. Taken together, we have demonstrated that miR-21 inhibits cell growth through regulating hTERT expression in a STAT3-dependent pathway in GBM cells. These results suggest that modulation of the mechanism responsible for miR-21 in GBM could be used as a therapeutic strategy for GBM treatment and warrants further investigation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3973. doi:10.1158/1538-7445.AM2011-3973
Are you serious about climate change, BMJ?
Authors: A. Inglis
Journal: BMJ (2011)

Periodically the BMJ tries to convey the importance with which it claims to regard the threat of climate change,1 and it boasts a fine sounding environmental policy proclaiming “BMJ Group [is] going green.”2

The BMJ continues to rely on the circulation of print copies when increasing numbers of readers have other options available: online through BMA membership and employer’s …
Abstract 2884: Standardized mangosteen fruit extract promotes cell cycle arrest and apoptosis of PC3 cells and inhibits tumor formation in athymic nude mice
Authors: Jeremy J. Johnson, Deeba N. Syed, Vaqar Adhami, John T. Rasmussen, Imtiaz A. Siddiqui, Hasan Mukhtar
Journal: Cancer Research (2010)

Abstract The purple mangosteen fruit (Garcinia mangostana) is native to southeast Asia and has been reported to contain a variety of health promoting properties that include antioxidant, anti-inflammatory, anit-microbial and anti-diabetic properties. The principle constituents isolated from the mangosteen fruit are the xanthones with well over 50 unique entities isolated to date. Here we report on the properties of a mangosteen fruit extract for cancer chemoprevention and/or chemotherapy using prostate cancer (PCa) as a model disease. Using a mangosteen fruit extract (MFE) a significant decrease in cell viability was observed against four different prostate cancer cell lines (LNCaP, CWR22, PC3, DU145). Several analytical standards were used to identify dietary xanthones by HPLC with alpha-mangostin found to be the most abundant xanthone of MFE representing approximately 59% of the extract. Next, we investigated the ability of MFE to promote cell cycle arrest by flow cytometry and observed a significant increase in cells in G1 phase when treated with 7.5 μg/mL of MFE after 24 hours. Further confirmatory studies were performed by western blot analysis of PC3 cells treated with MFE. To understand the possible interaction between xanthones and cyclins/cdks in a cell free system a biochemical based assay was performed and found to inhibit cyclinD1/CDK4 as well as other kinases. To determine the potential of MFE in an in vivo model we investigated the ability of MFE to inhibit colony formation of CWR22 and PC3 cells. MFE was found to completely inhibit colony formation of CWR22 cells at a concentration of 5 µg/mL. Next, using an athymic nude mouse model implanted with CWR22 cells mice were randomized to receive intraperitoneally the standardized mangosteen fruit extract (MFE) or placebo two times weekly with tumors measured twice weekly until tumors reached a volume of 1200 mm3. At the conclusion of the study the treatment group had a significantly smaller tumor with an average tumor volume of (143 mm3) compared to the placebo group (1135 mm3) representing an 87.5% smaller tumor in the treatment group. Based on these promising results we have evidence suggesting that MFE may be a promising agent for the prevention and/or treatment of prostate cancer. Further studies are needed to determine the potential of purified dietary xanthones from the mangosteen fruit for cancer chemoprevention and chemotherapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2884.
External pancreatic fistula treated by endoscopic ultrasound-guided drainage with a novel lumen-apposing metal stent mounted on a cautery-tipped delivery system
Authors: Franco Orellana, Fabia Attili, Santiago Andrade Zurita, Guido Costamagna, Alberto Larghi
Journal: Endoscopy (2015)

One of the most common causes of external pancreatic fistula is the iatrogenic manipulation of a complex pancreatic fluid collection concomitantly associated with a disconnected pancreatic duct [1] [2]. This situation can lead to the development of a high output (up to 400 mL/d) external pancreatic fistula that is difficult to manage and sometimes requires surgery [3].
Imaging Pelvic Inflammatory Disease with Tc-99m Ciprofloxacin
Authors: WONSICK CHOE, MOON WHAN IM, JUNE-KEY CHUNG, JAE MIN JEONG
Journal: Clinical Nuclear Medicine (2000)

The authors describe scintigraphic images of patients with pelvic inflammatory disease (PID) that were obtained using a Tc-99m–-labeled antibiotic, ciprofloxacin. Compared with control images, those of the patients with PID showed foci of significantly increased uptake in the regions corresponding to the areas of clinical symptoms. This imaging technique is considered valuable in persons with symptoms of PID in whom diagnosis is difficult. FIGURE 1Fig. 1: Scintigraphic images were obtained 1 hour after injection in a patient with bilateral adnexitis. Increased uptake is evident in both adnexa (arrows), where the patient had tenderness. Mildly increased uptake in the uterus (arrow) is also present. The diagnosis of PID depends mainly on clinical symptoms and signs, which are often lacking in objectivity. Although laparoscopic biopsy increases the diagnostic accuracy, it is seldom used because of its invasiveness (1). PID is often caused by bacterial infection, and an imaging technique exists that visualizes a focus of the bacterial infection (2–4). We used our custom-formulated Tc-99m ciprofloxacin for imaging in our patient.FIGURE 2Fig. 2: Scintigraphic images of the blood flow study.FIGURE 3Fig. 3: Scintigraphic images were obtained 1 hour after injection. The patient had a left tuboovarian abscess that was confirmed at surgery. There is increased uptake in the area of the left ovary (arrow) (with central photopenia) and in the uterus (arrow).FIGURE 4Fig. 4: Scintigraphic images of another patient (control) who had no clinical evidence of PID were obtained 1 hour after injection.
201 ASSESSMENT OF SEXUAL ACTIVITY AND CONJUGAL SATISFACTION AFTER LIVER TRANSPLANTATION IN MALE AND FEMALE PATIENTS
Authors: J. Klein, S.-N. Tran, A. Mentha-Dugerdil, E. Giostra, P. Majno, I. Morard, T. Berney, P. Morel, G. Mentha, C. Iselin, C. Toso
Journal: Journal of Hepatology (2012)
Changes in Renal Concentrating Ability Associated with Major Surgical Procedures
Authors: Herbert D. Gullick, Lawrence G. Raisz
Journal: New England Journal of Medicine (1960)

THE occurrence of acute renal failure after major surgery has centered attention on the effects of an operative procedure on renal function. Although data are available concerning the effects of anesthesia, operation and the postoperative state on urine formation, renal hemodynamics, water diuresis and electrolyte excretion,1 2 3 4 5 6 7 8 there is little information about the effect of major surgical procedures on renal concentrating ability. It has been suggested that measurement of concentrating ability is useful in differentiating postoperative oliguria due to dehydration from oliguria due to acute tubular necrosis.9 , 10 Concentrating ability is known to decrease transiently in normal subjects during hypotension11 or heavy . . .
THE TYPES OF POSTSYSTOLIC SHORTENING WERE RELATED TO TRANSMURAL NECROSIS AND MICROVASCULAR OBSTRUCTION IN ACUTE PHASE OF MYOCARDIAL INFARCTION: A CONTRAST STUDY WITH CARDIAC MAGNETIC RESONANCE
Authors: Hang Zhao, Ge Heng, Zhiqing Qiao, Jun Pu, Xuedong Shen, Ben He
Journal: Journal of the American College of Cardiology (2015)