tbuckley/cabot-search huggingface dataset:
Abstract 447: Hypoxia induces tumor cell escape from T cell-mediated immunity via up-regulation of B7-H1.
Authors: Ivraym B. Barsoum, Chelsea A. Smallwood, D. Robert Siemens, Charles H. Graham
Journal: Cancer Research (2013)

Abstract In the current research we describe a novel mechanism by which hypoxia contributes to tumour cell escape from lysis mediated by cytotoxic T lymphocytes (CTLs). Specifically, we show that when exposed to hypoxia for 24 h, human DU145 prostate and MDA-MB-231 breast cancer cells, as well as mouse B16-OVA melanoma cells, increased their expression of the immune inhibitory ligand B7-H1 at both the mRNA and protein levels in a manner dependent on hypoxia-inducible factor-1α (HIF-1α). Moreover, our findings revealed that the hypoxia-induced up-regulation of B7-H1 expression in tumour cells resulted in increased tumour cell resistance to lysis mediated by primed CTLs. We previously demonstrated that activation of nitric oxide (NO) signalling blocks HIF-1α accumulation in tumour cells exposed to hypoxia. Here we show that the NO mimetic glyceryl trinitrate (GTN; 10 nM) prevented the hypoxia-induced up-regulation of tumour cell B7-H1 expression as well as the increased resistance to CTL-mediated lysis. Furthermore, co-culture studies revealed that the increased expression of B7-H1 following exposure of tumour cells to hypoxia led to the apoptotic death of CTLs as well as Jurkat leukemia cells in a manner that could be prevented by blocking B7-H1, programmed cell death-1 receptor (B7-H1 receptor expressed by T cells) or by incubation with low concentrations of GTN. These results indicate that a potential immunotherapy of cancer could involve interference with B7-H1 function in hypoxic tumour cells via administration of NO mimetics. Citation Format: Ivraym B. Barsoum, Chelsea A. Smallwood, D. Robert Siemens, Charles H. Graham. Hypoxia induces tumor cell escape from T cell-mediated immunity via up-regulation of B7-H1. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 447. doi:10.1158/1538-7445.AM2013-447
O107 ANTIVIRAL ACTIVITY OF VARIOUS INTERFERONS (IFNS) AND INFLAMMATORY CYTOKINES IN RELEVANT HEPATOCYTE MODELS OF PERSISTENT HEPATITIS B VIRUS (HBV) INFECTION
Authors: N. Isorce, B. Testoni, S. Luangsay, M. Ait-Goughoulte, M. Gruffaz, C. Zannetti, U. Hasan, T. Henry, D. Durantel, F. Zoulim
Journal: Journal of Hepatology (2014)
Multifocal Pott’s Disease (Tuberculous Spondylitis) Incidentally Detected on Tc-99m MDP Bone and Ga-67 Citrate Scintigraphy in a Patient with Diabetes
Authors: Seong-Jang Kim, Ju-Won Seok, In-Ju Kim, Yong-Ki Kim, Dong-Soo Kim
Journal: Clinical Nuclear Medicine (2003)

Pott’s disease is an uncommon extrapulmonary form of tuberculosis. Delay in diagnosis and management may cause serious complications. The authors describe Pott’s disease incidentally detected on Tc-99m MDP bone and Ga-67 imaging in a patient with diabetes. Tc-99m MDP bone scintigraphy showed intensely increased uptake in the lower cervical spine and lumbosacral regions. Ga-67 scintigraphy revealed intensely increased uptake corresponding to the areas noted on Tc-99m MDP bone scintigraphy. Magnetic resonance imaging showed destructive lesions in the C5-C6 and L5-S1 intervertebral discs with destruction of adjacent end plates. Biopsy of the lumbosacral area was guided by computed tomography, and histologic examination of the bone specimen showed caseation, giant cells, and acid-fast bacilli. Posterior decompression and posterolateral spinal fusion with bone grafts were performed. Antituberculous chemotherapy with isoniazid, rifampicin, pyrazinamide, and ethambutol was started. The patient showed remarkable relief of symptoms during a period of 9 months of therapy. Both Tc-99m MDP bone and Ga-67 imaging can offer the convenience of screening the entire body to detect multiple sites of Pott’s disease.
AB0977 Prevalence of Subclinical Synovitis Detected by Ultrasound in Rheumatoid Arthritis and Psoriatic Arthritis Patients Receiving Anti-TNF Therapy with Extended Interval of Administration: Table 1.
Authors: J.M. Senabre Gallego, J. Rosas, E. Salas Heredia, G. Santos Soler, F. Llinares Tello, C. Santos Ramírez, M. Sánchez Barrioluengo, X. Barber, R. Ortega, A. Pons, C. Cano, M.I. Lorente
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Objectives</h3> To estimate the prevalence of subclinical synovitis detected by ultrasound in rheumatoid arthritis (RA) and psoriatic arthritis (PsA) patients in clinical remission receiving anti-TNFα therapy with extended interval of administration (EIA). <h3>Methods</h3> Prospective observational study. Population: Patients diagnosed with RA and PsA, receiving anti-TNFα and being in clinical remission. 12-joint ultrasound assessment (elbows, wrists, 2nd and 3rd metacarpo-phalangeal, knees and ankles)<sup>1</sup> was performed, evaluating synovitis through B-mode (BM) and Color Doppler signal (CD), both by semiquantitative scale from 0 to 3 points (Esaote MyLab 25). Subsequently, a BM and CD score was calculated, summing the highest score obtained from any one of the synovial sites evaluated at each joint to a maximum of 36 points. The sonographer was blinded to the clinical and laboratory data. <h3>Results</h3> 34 patients were included in the study, 70.6% were women, mean age was 60 years [32-81] and mean duration of disease was 14.6 years [3-46]. The diagnosis was RA in 30 patients (88.2%) and APs in 4 (11.7%). In 86.7% of patients with RA rheumatoid factor was positive, and in 76.7% the citrullinated protein antibody was positive. Ultrasound assessment prior to EIA was available in 24 patients, and after EIA in 23 patients. Clinical activity and ultrasound scores are summarized in Table 1. Ultrasound detects some synovitis by CD in 52% of EIA patients, and by BM in 78% of EIA patients. Nevertheless most of them had a low CD score (average 1.3 out of 36 points). The EIA treatments were etanercept (ETN) in 13 patients and adalimumab (ADA) in 10 patients, with the following patterns: ETN/10 days (11 pat.), ETN/14 days (1 pat.), ETN/15 days (1 pat.), ADA/18 days (6 pat.), ADA/21 days (2 pat.), ADA/17 days (1 pat.), ADA/30 days (1 pat.). Six patients (24%) never began EIA due to clinical decision, and 7 (29%) had to return to the standard administration pattern due to worsening of disease activity. <h3>Conclusions</h3> Some synovitis was detected by Color Doppler ultrasound in 52% of patients in clinical remission receiving anti-TNFα therapy with extended interval of administration. Most of them had a low Color Doppler ultrasound score. Synovial hypertrophy was detected by B-mode ultrasound in 78% of them. <h3>References</h3> Naredo E. Arthritis Rheum 2008,59:515-22. <h3>Acknowledgements</h3> This work was supported by a grant from Fundaciόn Española de Reumatología. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2990
Abstract 5587: Serum HPV 6, 11, 16 and 18 neutralizing antibody detection by multiplex liquid array system (luminex)
Authors: Heejin Bae, Sumi Bae, Keun Ho Lee, Sei Jun Han, Woong Shick Ahn
Journal: Cancer Research (2010)

Abstract Recently two prophylactic HPV vaccines including types 6, 11, 16 and 18 are clinically available. Detection of HPV DNA in cervix and neutralizing antibody in serum are important before and after vaccination. A luminex technology facilitates the simultaneous evaluation of multiple immune mediators with advantages of high throughput, small sample volume, lower cost and short time than ELISA. We are already developed HPV DNA in cervix detection system using multiplex liquid array (Cancer science, 2007) and now established detection system using luminex. For production the antigen, HPV 6, 11, 16 and 18 L1 genes were isolated from their genomic DNA by PCR, cloned into pGEM vector with simian virus 40 (SV 40) large T-antigen and expressed in E. coli. Purified GST fusion recombinant proteins are coupled to microbeads, respectively. The characteristics of the assay were optimized using monoclonal antibody (MAb) specificity, validated with several distinct serum panels and used to determine the serum neutralizing antibody. Using this system, we tested 40 females who vaccinated quadrivalent prophylactic HPV vaccine 6 month ago (20) and sexually unexposed group in teenager (20). The value of neutralizing antibody was average 1800 (±200) MFI in vaccinated group. This high-throughput multiplex liquid array system is highly sensitive. We can use population based validation and combination with HPV DNA test using luminex. By using multiplex luminex beads coupled HPV antigen, we can check whether neutralizing antibodies formation or not, and measure the titers of antibodies to each subtype of HPV. We found one possibility to check up the HPV DNA and serum neutralizing antibody before and after HPV prophylactic vaccination. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5587.
SP0081 Fifteen years of experience with patient involvement in research. What has been achieved?
Authors: J. Kirwan
Journal: Annals of the Rheumatic Diseases (2013)

At our Academic Rheumatology Unit there we have recorded 37 different patients who have been involved in contributions to the development and implementation of our research programme over the last 15 years. They have contributed on 147 different occasions including providing advice and support, helping to write grant applications, making contributions to protocol development, analysing results and interpreting results. Our Patient Research Partners have be co-authors on 10 publications. In addition, through my role as Patient Coordinator of the OMERACT Group, I have involved 58 patients in this 2-yearly international conference since 2000. Involving patients has helped to direct our research efforts, and has helped to ensure we have grasped the nature of the research questions we want to address. I will provide a local example of a PhD project where progress was crucially dependant on patient insights, and where patient involvement in the work has helped us to appreciate them. I will refer to the international example of the development of fatigue as an outcome measure in rheumatoid arthritis, which was initiated and facilitated at all stages by patient partner involvement. Working together, we have achieved a step improvement in the quality and relevance of research into the consequences of arthritis and some new approaches to treatment, and we have learned which techniques are best able to support patient research partners in making their contributions. <h3>Disclosure of Interest</h3> None Declared
Abstract 1600: Molecular Characterization of <i>PAX5</i> alterations in Adult <i>BCR-ABL1</i>-Positive Acute Lymphoblastic Leukemia (ALL)
Authors: Annalisa Lonetti, Ilaria Iacobucci, Cristina Papayannidis, Anna Ferrari, Viviana Guadagnuolo, Daniela Cilloni, Francesca Messa, Stefania Paolini, Sabina Chiaretti, Simona Soverini, Giuseppe Saglio, Fabrizio Pane, Michele Baccarani, Robin Foà, Giovanni Martinelli
Journal: Cancer Research (2010)

Abstract Background: Recently, a genome-wide analysis using single nucleotide polymorphism (SNP) microarrays, identified genetic alterations targeting PAX5 in over 30% of pediatric acute lymphoblastic leukemia cases (Mullighan et al. Nature 2008). However, the occurrence of PAX5 alterations has not been investigated in adult BCR-ABL1-positive ALL. Aim: To characterize mutations and rearrangements on 9p involving PAX5 in adult BCR-ABL1-positive ALL. Patients and Methods: Eighty-nine patients with de novo adult BCR-ABL1-positive ALL were enrolled into institutional (n = 15) or Gruppo Italiano Malattie Ematologiche Maligne dell'Adulto Acute Leukemia Working Party (n = 74) clinical trials and after obtaining informed consent their genome was analyzed by SNP arrays (Affymetrix 250K NspI and SNP 6.0), FISH, genomic PCR and resequencing. Results: SNP array analysis identified a loss of PAX5 in 29 patients (33%). In all cases the deletion was heterozygous. Four patterns of PAX5 deletion were observed: focal deletion involving only the PAX5 gene in one case (3%); deletions involving only a portion of PAX5 and both telomeric and centromeric genes in 11 cases (38%) with a median size of 310 kb (range: 101 kb-16,395 kb); broader deletions involving the entire PAX5 locus and a variable number of flanking genes in 10 patients (34%) with a median size of 1,999 kb (range: 567 kb-1,208 kb); deletion of all chromosome 9 or 9p in 7 patients (24%). These deletions may result in PAX5 haploinsufficiency or altered gene expression. In one patient the deletion of PAX5 involved only a subset of exons (2-6) resulting in an alternative transcript encoding a prematurely truncated protein lacking key PAX5 functional domains. To investigate the consequences of genomic PAX5 alteration, quantitative PCR (q-PCR) was used: genomic alterations on 9p13.2 lead to a significant down-modulation at the transcript level of Pax5. Normal and deleted PAX5 subgroups were also investigated for point mutations, analyzing each exon by direct sequencing, but we did not found nucleotide substitutions. Conclusion: PAX5 deletions, but not point mutations, are frequent events in adult BCR-ABL1 positive ALL and are the main mechanism of inactivation of PAX5 in BCR-ABL1 positive ALL. Supported by: European LeukemiaNet, AIL, AIRC, FIRB 2006, Strategico di Ateneo, GIMEMA Onlus. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1600.
Liposomal Amphotericin B for Empirical Therapy in Patients with Persistent Fever and Neutropenia
Authors: Thomas J. Walsh, Robert W. Finberg, Carola Arndt, John Hiemenz, Cindy Schwartz, David Bodensteiner, Peter Pappas, Nita Seibel, Richard N. Greenberg, Stephen Dummer, Mindy Schuster, William E. Dismukes, John S. Holcenberg
Journal: New England Journal of Medicine (1999)

In patients with persistent fever and neutropenia, amphotericin B is administered empirically for the early treatment and prevention of clinically occult invasive fungal infections. However, breakthrough fungal infections can develop despite treatment, and amphotericin B has substantial toxicity.
"Elephantine" Calf Swelling After Groin Resection and Radiation Therapy for Vulvar Carcinoma
Authors: J D Slavin, I O Engin, R P Spencer, J A Vento
Journal: Clinical Nuclear Medicine (1999)

At age 46 years, a woman with carcinoma of the left vulva underwent surgical resection, groin dissection, and therapeutic irradiation. Some 25 years later, whole-body imaging with Tc-99m MDP showed enlargement of the left lower extremity in an unusual pattern (greater in the calf) and of elephantine proportions.
Prehospital Ventricular Defibrillation
Authors: Richard R. Liberthson, Eugene L. Nagel, Jim C. Hirschman, Sidney R. Nussenfeld
Journal: New England Journal of Medicine (1974)

The subsequent course of 301 subjects with prehospital ventricular fibrillation detected by rescue units was analyzed. Defibrillation was successful in 199. Of these patients, 98 died before admission; of 101 who were hospitalized, 59 died in the hospital, and 42 were discharged alive. Initially rapid post-defibrillation heart rates and atrial fibrillation or sinus tachycardia were associated with good survival in contrast to slow rates and idioventricular or junctional rhythms. Among hospitalized patients ventricular fibrillation or ventricular tachycardia recurred in 57 per cent and in most within 24 hours. Acute myocardial infarction was diagnosed in 35 per cent, and ischemia alone (predominantly of the anterior wall) in 32 per cent. In 17 per cent no acute myocardial changes evolved. Congestive heart failure occurred in 63 per cent, cardiogenic shock in 25 per cent, and pulmonary complications in 42 per cent. Of the 42 who were discharged, 60 per cent returned to pre-arrest status; 28 per cent had mild, and 12 per cent had severe neurologic deficit. Of hospital deaths, 40 per cent were within one day, and 75 per cent within one week. Mean survival for discharged patients was 12.7 months. Regardless of arrhythmia prophylaxis, 28 per cent died suddenly after discharge. (N Engl J Med 291:317–321, 1974)
PHYSICIAN DECISION MAKING IN ANTICOAGULATING ATRIAL FIBRILLATION: A PROSPECTIVE SURVEY EVALUATING A PHYSICIAN ALERT SYSTEM FOR ATRIAL FIBRILLATION DETECTED ON CARDIAC IMPLANTABLE ELECTRONIC DEVICES
Authors: Justin Cloutier, Clarence Khoo, Brett Hiebert, Anthony Wassef, Colette Seifer
Journal: Journal of the American College of Cardiology (2016)
1143 DISCONTINUATION OF PEGYLATED INTERFERON FOR REASONS OTHER THAN LACK OF EFFICACY IN UNITED STATES VETERANS WITH CHRONIC HEPATITIS C
Authors: J. LaFleur, R.E. Nelson, R. Hoop, P. Pandya, T. Morgan, C.L. Hayden, Y. Yao, F. Hamzeh
Journal: Journal of Hepatology (2012)
Abstract 1230: Nicotine induces expression of integrin-linked kinase in human lung carcinoma cells through A7 nAChR-mediated inhibition of AP-2A
Authors: XiaoRong Zhong, Ying Zheng, XiaoJuan Sun, Jeffrey D. Ritzenthaler, Jesse Roman, Shouwei Han
Journal: Cancer Research (2010)

Abstract We previously demonstrated that nicotine stimulates non small cell lung carcinoma (NSCLC) cell proliferation through nicotinic acetylcholine receptor (nAChR)-mediated induction of fibronectin expression. However, the mechanisms by which this agent affects human lung cancer occurrence and progression remain incompletely elucidated. Here, we show that nicotine increases the expression of integrin-linked kinase (ILK), a unique intracellular adaptor and kinase that has been implicated in the regulation of cancer cell growth/survival. This effect was blocked by the α7 nAChR antagonist, α-bungarotoxin, and by α7 nAChR siRNA. Silencing of ILK blocked the stimulatory effect of nicotine on phosphorylation of the PI3-K downstream target Akt and on cell growth. Furthermore, nicotine increased ILK gene promoter activity through inhibition of transcription factor AP-2α protein expression. Silencing of AP-2α enhanced, while exogenous expression of AP-2α attenuated, the stimulatory effect of nicotine on ILK expression. These findings appear to be relevant in vivo since we found that nicotine treatment increased tumor growth in animal models. Collectively, our results demonstrate that nicotine increases ILK gene expression through α7 nAChR-mediated inhibition of AP-2α followed by stimulation of PI3-K/Akt signaling. In turn, this leads to increased NSCLC cell growth. This study unveils a novel mechanism by which nicotine promotes human lung carcinoma cell growth/survival. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1230.
Abstract LB-386: The NuRD complex cooperates with DNMTs to maintain silencing of colorectal tumor suppressor genes.
Authors: Yi Cai, Ernst-Jan Geutjes, Paul Roepman, Li-Rong Yu, Janneke V. Blijswijk, Wei Wang, Helai Mohammad, Iris de Rink, Stephen Baylin, Rene Bernards
Journal: Cancer Research (2012)

Abstract Many Tumor Suppressor Genes (TSGs) are silenced through synergistic layers of epigenetic regulation including abnormal DNA hypermethylation of promoter CpG islands, repressive chromatin modifications and enhanced nucleosome deposition over transcription start sites. Many of the protein complexes responsible for silencing of such TSGs remain to be identified. A subset of silenced TSGs controlling key regulatory signaling pathways in colorectal cancer cells can be partially reactivated by in cells disrupted for the DNA methyltransferase 1 and 3B (DNMT1 and 3B) or by DNMT inhibitors (DNMTi). Herein, we used proteomic and functional genomic approaches to identify additional proteins that cooperate with DNMTs in silencing these key silenced TSGs in colon cancer cells. We discovered that DNMTs and the core components of the NuRD nucleosome remodeling complex, chromo domain helicase DNA-binding protein 4 (CHD4), histone deacetylases 1 and 2 (HDAC1 and 2), occupy the promoters of several of these key hypermethylated TSGs and physically and functionally interact to maintain their silencing. Consistent with this, we find an inverse relationship between expression of HDAC1 and 2 and these TSGs in a large panel of primary colorectal tumors. We demonstrate that this DNMT-NuRD complex maintains the silencing of several negative regulators of the WNT signaling pathway. We find that depletion of CHD4 is synthetic lethal with DNMT inhibition in correlation with reactivation of TSGs, suggesting that their combined inhibition may be beneficial for the treatment of colon cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-386. doi:1538-7445.AM2012-LB-386
ONE YEAR OUTCOMES AFTER SWITCHING FROM A BIVALIRUDIN-FIRST STRATEGY TO A HEPARIN-FIRST STRATEGY FOR ALL PERCUTANEOUS CORONARY INTERVENTIONS IN A SINGLE-CENTER CARDIAC CATHETERIZATION LAB
Authors: Rahul Jaswaney, Christopher End, Melissa Caughey, Patricia Sudar, Michael Yeung, Prashant Kaul, George Stouffer, John Vavalle
Journal: Journal of the American College of Cardiology (2016)
Biliary cast syndrome and liver abscesses complicating composite multivisceral transplantation
Authors: Fernanda Martins, Gustavo De Paulo, Erika Macedo, Angelo Ferrari
Journal: Endoscopy (2014)

A 59-year-old woman underwent composite multivisceral transplantation as a result of cryptogenic cirrhosis and extensive thrombosis of the splanchnic vessels. She presented with obstructive jaundice with elevated liver enzymes 48 days later. A computed tomography (CT) scan ([Fig. 1]) disclosed two large hepatic abscesses and dilated intra- and extrahepatic bile ducts. She underwent interventional percutaneous drainage and administration of antibiotics for the abscesses and was referred for endoscopic retrograde cholangiopancreatography (ERCP) on the following day. At ERCP, a dilated common bile duct was found with a large intraductal filling defect with extension into the intrahepatic system ([Fig. 2]). Endoscopic biliary sphincterotomy was performed followed by balloon retrieval of biliary debris. Bile duct clearance was not possible and therefore plastic stents were placed. Laboratory test results did not improve. A percutaneous transhepatic cholangiogram was performed 2 weeks later demonstrating a very dilated and severely irregular biliary tree filled with casts ([Fig. 3]). Although the hepatic abscesses disappeared, the severe biliary tree distortion remained unresolved ([Fig. 4]) despite repeated interventions. The graft damage was progressive and eventually the patient died after multiple complications.