tbuckley/cabot-search huggingface dataset:
Vitamin D levels, triple-negative histology, and geography in breast cancer: A retrospective analysis of a large database of oncology practices in the United States.
Authors: Joyce Georges Habib, Janet L. Espirito, Robyn K. Harrell, Brian Turnwald, Joyce O'Shaughnessy, Ervin H. Epstein, Debra A. Patt
Journal: Journal of Clinical Oncology (2014)

17 Background: The UVB-vitamin D-breast cancer (BC) hypothesis is supported by ecological studies demonstrating an inverse correlation between sunlight exposure and BC incidence and mortality. Observational studies also favor an inverse association between vitamin D status and BC risk, recurrence and mortality. Yet controversies remain regarding the role of vitamin D in BC. We examined associations between vitamin D levels, geographic location, clinical, and pathologic characteristics of BC patients (pts). Methods: This was a retrospective analysis from the electronic health record database of pts diagnosed with BC between 1/2007 and 5/2013 across US Oncology Network practices categorized based on their geographic location into: northern (&gt; 40° N), central (35 to 40°N) and southern (&lt; 35°N) latitude. We collected age at diagnosis, BMI, smoking history, stage, estrogen receptor (ER), progesterone receptor (PR), HER2 status, and the first documented serum 25-hydroxyvitamin D (25-(OH)D) level, categorized as 30 optimal. Statistical comparison was performed using Chi-squared tests for categorical variables and Kruskal-Wallis tests for continuous variables. Logistic regression was used to predict the likelihood of vitamin D deficiency. Results: 20,338 BC pts with a documented vitamin D level were identified. Mean age at diagnosis was 58. Stage and receptor status distribution were: 8%, 41%, 32%, 11% and 4% for stage 0, I, II,III, and IV respectively; 63%, 13%, and 10% for ER+/HER-2-, HER-2+ and TNBC, respectively. 17.6% and 27.8% of pts had deficient or suboptimal vitamin D levels. The covariates of age &lt; 60 years (OR 1.24), advanced stage (OR 1.32 stage II, OR 1.51 stage III, and OR 1.81 stage IV), TNBC (OR 1.45), BMI ≥ 25 (OR 2.02), current smoker (OR 2.11), and lower latitudes (OR 1.36 and 1.19 for central and southern latitude respectively) were independent predictors of a first documented vitamin D deficiency in a multivariate model. Conclusions: Vitamin D deficiency may be associated with TNBC and central and southern latitudes. The possible influences of differential vitamin D supplementation and timing of testing require further investigation.
Secondary Prophylaxis with rFVIIa Improves Quality of Life of Hemophilia Patients with Inhibitors and Frequent Bleeds.
Authors: Barbara A. Konkle, Liselotte S. Ebbesen, Guenter K.H. Auerswald, Ute Friedrich, Rolf C.R. Ljung, Harold R. Roberts, W. Keith Hoots
Journal: Blood (2006)
Accumulation of Myeloid-Derived Suppressor Cells in Bone Marrow in Multiple Myeloma Induces Tumor-Specific Immune Suppression and Promotes Tumor Growth
Authors: Indu R Ramachandran, Anna Martner, Thomas C Condamine, Alexandra Pisklakova, Tess Chase, Dmitry Gabrilovich, Yulia Nefedova
Journal: Blood (2012)
Differential Maintenance of Cell Cycle Status of Human Bone Marrow- and Umbilical Cord Blood-Derived CD34+ Cells after Homing to the Bone Marrow Microenvironment.
Authors: Bradley A. Poteat, Brahmananda Reddy Chitteti, Karen E. Pollok, Attaya Suvannasankha, Edward F. Srour
Journal: Blood (2008)
Quantitative HLA Class I Expression and KIR-Mismatch between NK-Cell-Donor and Patient Predict NK Cell Mediated Lysis of Leukemic Cells from Children with Acute Lymphatic Leukemia.
Authors: Matthias M. Pfeiffer, Michael Schumm, Klaus Dietz, Tobias Feuchtinger, Rupert Handgretinger, Peter J. Lang
Journal: Blood (2006)
Brief Report: <i>Dialister</i> as a Microbial Marker of Disease Activity in Spondyloarthritis
Authors: Raul Y. Tito, Heleen Cypers, Marie Joossens, Gaëlle Varkas, Liesbet Van Praet, Elien Glorieus, Filip Van den Bosch, Martine De Vos, Jeroen Raes, Dirk Elewaut
Journal: Arthritis & Rheumatology (2016)

Objective Dysbiosis of the intestinal microbiota has been widely established in inflammatory bowel disease (IBD). There is significant clinical and genetic overlap between spondyloarthritis (SpA) and IBD, and up to 50% of all patients with SpA exhibit microscopic signs of bowel inflammation, often bearing particular resemblance to early Crohn's disease, a subtype of IBD. This study was undertaken to assess the relationship between intestinal microbial composition, gut histology, and disease activity markers in SpA. Methods Gene analysis by 16S ribosomal RNA amplicon sequencing was used to compare the microbial composition in ileal and colonic biopsy specimens from 27 patients with SpA (14 with microscopic bowel inflammation, 13 without) and 15 healthy control subjects (ileal samples from all 15 subjects and colonic samples from 6). Spearman's rank correlation tests were used to assess correlations of the microbial composition with disease activity measures. Results The intestinal inflammation status (histologically normal versus acute or chronic inflammation) was strongly associated with the mucosal microbiota profile of patients with SpA. In inflamed biopsy tissue, the detected bacterial community composition clustered separately from that in noninflamed biopsy tissue ( P &lt; 0.05 by permutational multivariate analysis of variance, using hierarchical clustering on Bray–Curtis distances). Interestingly, abundance of the genus Dialister was found to be positively correlated with the Ankylosing Spondylitis Disease Activity Score (Spearman's rho = 0.62, false discovery rate–corrected q &lt; 0.01). This finding was further supported by the low frequency of Dialister observed in noninflamed ileal and colonic biopsy tissue from patients with SpA and healthy controls. Conclusion These findings demonstrate a significant difference in the intestinal microbial composition in patients with SpA who have microscopic gut inflammation compared to those without microscopic gut inflammation. Moreover, Dialister may represent a potential microbial marker of disease activity in SpA.
Optimization of One-Stage Coagulation Factor Assays: Improved Accuracy and Precision over an Extended Linear Range.
Authors: Janos Polgar, Jack Havelick, Qun Li, Nathalie Laurent, Mahesh Unalkat, Denise Matte, Zina Gruman, Pamela Haley, Edith Doenges, Christopher Holland, Istvan Csapo, Colleen Crowly, Mary Doyle, Mark Triscott
Journal: Blood (2007)
Long-Term Outcome for De Novo or Minimally Treated Burkitt-Type Lymphoma/Leukemia (BL/B-ALL) After Therapy with Hyper-CVAD and Rituximab
Authors: Deborah A. Thomas, Susan O'Brien, Jorge E. Cortes, Stefan Faderl, William G. Wierda, Guillermo Garcia-Manero, Elias Jabbour, Alessandra Ferrajoli, Srdan Verstovsek, Jan A. Burger, Rebecca Garris, Jorge Romaguera, Fredrick Hagemeister, Hagop Kantarjian
Journal: Blood (2010)
A Phase II Study of Alemtuzumab in the Treatment of Plasma Cell Disorders.
Authors: Lijo Simpson, Shaji Kumar, Jacob Allred, Martha Lacy, Angela Dispenzieri, Suzanne Hayman, Steven Zeldenrust, S. Vincent Rajkumar, John A. Lust, Philip R. Greipp, Morie Abraham Gertz, Thomas E. Witzig
Journal: Blood (2006)
STAT3-Mutations Indicate the Presence of Subclinical Self-Reactive Cytotoxic T Cell Clones in Aplastic Anemia and Myelodysplastic Syndromes
Authors: Andres Jerez, Michael J. Clemente, Hideki Makishima, Hanna L M Koskela, Inés Gómez-Seguí, Thomas Olson, Kathy McGraw, Bartlomiej Przychodzen, Austin G Kulasekararaj, Manuel G. Afable, Holleh D Husseinzadeh, Edward P Evans, Naoko Hosono, Francis LeBlanc, Sonja Lagström, Dan Zhang, Pekka Ellonen, Alan E. Lichtin, Aleksandra Wodnar-Filipowicz, Ghulam J. Mufti, Alan F. List, Satu Mustjoki, Thomas P. Loughran, Jaroslaw P. Maciejewski
Journal: Blood (2012)
BMA calls for urgent meeting with prime minister to discuss NHS crisis
Authors: Gareth Iacobucci
Journal: BMJ (2017)

The BMA’s chair of council, Mark Porter, has requested an urgent meeting with Theresa May to discuss the crisis in the NHS in England after Downing Street’s controversial decision to point the finger of blame at GPs for the current pressure on hospital emergency departments.1

In a letter to the prime minister Porter accused May of “playing down” the crisis and deliberately trying to scapegoat GPs to distract from the situation in the NHS. He warned that …
Schedule Dependent Effects of Adoptively Transferred Autologous T Cells After AutoSCT for Myeloma: Accelerated Immune Recovery Is Associated with Improved Event-Free Survival.
Authors: Aaron P. Rapoport, Nicole A. Aqui, Edward A. Stadtmauer, Dan T. Vogl, Hong-Bin Fang, Stephen Janofsky, Jan Storek, Anne Chew, Elizabeth Veloso, Gorgun Akpek, Saul Yanovich, Ming T. Tan, Yin Wu, Alan Cross, Sunita Philip, Heather Murphy, Rita Bhagat, Yiping Liu, Bruce Levine, Robert H. Vonderheide, Carl H. June
Journal: Blood (2009)
Increased Osteoprotegerin Levels in Response to Increased Bone Resorption in AL Amyloidosis: Comparisons with Bone Remodeling of Multiple Myeloma.
Authors: E. Kastritis, Meletios Athanasios Dimopoulos, M. Mihail, E. Mihali, M.C. Kyrtsonis, S. Delimpassi, E. Katodritou, N. Anagnostopoulos, G. Kokkini, K. Zervas, K. Anargyrou, P. Panagiotidis, E. Terpos
Journal: Blood (2007)
A Phase I study of LFB-R603, a Novel Anti-CD20 Antibody, in Patients with Relapsed Chronic Lymphocytic Leukemia (CLL)
Authors: Guillaume Cartron, Bruno Cazin, Bertrand Coiffier, Stéphane Leprêtre, Pierre Feugier, Thérèse Aurran, Guylène Chartier, Alain Sadoun, Vincent Ribrag
Journal: Blood (2010)
Fluctuating Values of Molecular Residual Disease (MRD) without Molecular Progression After Imatinib Discontinuation in Patients (pts) with Chronic Myeloid Leukemia (CML) Who Have Maintained Complete Molecular Response: Implications for Re-Treatment Criteria and Role of Prior Interferon Therapy. A Pilot Study of the French CML Group (FILMC),
Authors: Philippe Rousselot, Pascale Cony Makhoul, Delphine Rea, Philippe Agape, Franck E Nicolini, Laurence Legros, Michel Tulliez, Lydia Roy, Aude Charbonnier, Joelle Guilhot, Francois Guilhot, Francois-Xavier Mahon
Journal: Blood (2011)
The Use of Autologous LMP2-Specific Cytotoxic T Lymphocytes (CTL) for the Treatment of Relapsed EBV-Positive Hodgkin Disease and Non-Hodgkin Lymphoma.
Authors: Catherine M. Bollard, Elizabeth Buza, Helen Huls, Teresita Lopez, Stephen Gottschalk, George Carrum, Vicky Gresik, Jeff Chang, Adrian P. Gee, Malcolm K. Brenner, Cliona M. Rooney, Helen E. Heslop
Journal: Blood (2005)
Cardiac Iron Overload Causes Clinically Evident Heart Failure and Arrhythmia in Sickle Cell Anemia Patients: Evidence From Three Cases
Authors: Bhakti P. Mehta, Vasilios Berdoukas, Mammen Puliyel, Adam Bush, Thomas Hofstra, Susan Claster, Anne Nord, Susan Carson, Tatiana Hernandez, Ani Dongelyan, John C Wood, Thomas D. Coates
Journal: Blood (2011)
Impact of Autologous Stem Cell Transplantation and/or Rituximab on Outcome of Patients with Relapsed Follicular Lymphoma- Retrospective Analysis of 2 Randomized Trials of the German Low Grade Lymphoma Study Group (GLSG).
Authors: Oliver Weigert, Atakan Uysal, Bernd Metzner, Michael Pfreundschuh, Norbert Schmitz, Hannes Wandt, Christian Peschel, Eva Hoster, Michael Unterhalt, Wolfgang Hiddemann, Martin Dreyling
Journal: Blood (2008)
Heterogeneity of the εγδβ Thalassemias: Characterization of 3 Novel English Deletions.
Authors: Helen Rooks, Jean Bergounioux, Laurence Game, James P. Close, Steve Best, Tania Senior, Susan Height, Thompson Richard, Hadzic Nedim, Paula Bolton-Maggs, Swee Lay Thein
Journal: Blood (2004)
Alternate Splicing Is a Frequent Event and Impacts Clinical Outcome in Myeloma: A High-Density Exon Array Analysis of Uniformly Treated Newly-Diagnosed Myeloma Patients
Authors: Nikhil C. Munshi, Cheng Li, Stephane Minvielle, Samir B Amin, Philippe Moreau, Florence Magrangeas, Kenneth C Anderson, Herve Avet-Loiseau
Journal: Blood (2008)
Nilotinib Is Associated with Minimal Cross Intolerance to Imatinib in Patients with Imatinib-Intolerant Philadelphia-Positive (Ph+) Chronic Myelogenous Leukemia (CML) in Either Chronic Phase (CP) or Accelerated Phase (AP).
Authors: Jorge Cortes, Elias Jabbour, Andreas Hochhaus, Philipp le Coutre, Michele Baccarani, Kapil N. Bhalla, Gert Ossenkoppele, Norbert Gattermann, Ariful Haque, Neil Gallagher, Francis Giles, Hagop M. Kantarjian
Journal: Blood (2007)
Which Patients Would Be Willing to Change Anticoagulation From Vitamin-K Antagonists to a New Oral Anticoagulant?
Authors: Job Harenberg, Svetlana Marx, Nadja Abou-Ayash, Christophe Kremer, Vera Hoeing, Katrin Schirk, Christel Weiss, Lutz Froelich
Journal: Blood (2011)
Nonalcoholic fatty liver disease in adolescents and young adults: The next frontier in the epidemic
Authors: Iliana Doycheva, Kymberly D. Watt, Naim Alkhouri
Journal: Hepatology (2017)

Nonalcoholic fatty liver disease (NAFLD) is a significant health burden in adolescents and young adults (AYAs) which has substantially risen in prevalence over the last decades. The occurrence of NAFLD parallels high rates of obesity and metabolic syndrome in this age group, with unhealthy lifestyle also playing an independent role. Genetic factors, sex, and ethnicity should be considered in a risk stratification model. NAFLD and nonalcoholic steatohepatitis (NASH) in AYAs often go unrecognized and, if untreated, can progress eventually to cirrhosis requiring liver transplantation (LT) before the age of 40. Recently, NASH has increased as an indication for LT in this age group. Important knowledge gaps include the feasibility of noninvasive diagnostic tests and imaging modalities as well as uncertainty about unique histological features and their predictive value. Future clinical trials focused on AYAs are needed to determine effectiveness of therapies. Tools for increasing awareness and prevention of NAFLD in AYAs are greatly needed. (H epatology 2017;65:2100‐2109).
Heat Shock Protein 90 (Hsp90) Activity Status Is An Independent Prognostic Marker in Chronic Lymphocytic Leukemia (CLL) and Correlates with IgVH Mutational Status, ZAP-70 Expression and Time to First Treatment
Authors: Januario E. Castro, Juan S. Barajas-Gamboa, Julio A. Diaz-Perez, Lina M. Ariza-Serrano, Johanna Melo-Cardenas, Mauricio Rodriguez, Mauricio Urquiza, Thomas J. Kipps
Journal: Blood (2011)
Influence of Different Components of the Tumor Microenvironment on Human Patient-Derived Lymphoma Cell Engraftment in Immmunodeficient Mice
Authors: Cordula Tschuch, Kerstin Klingner, Dorothee Lenhard, Cyrill Rentsch, Christian Wetterauer, Lukas Bubendorf, Tatjana Vlajndic, Milena Pantic, Lisa K Isbell, Nikolas von Bubnoff, Teppo Haapaniemi, Julia B. Schueler
Journal: Blood (2015)
Targeted Sequencing of Relapsed/Refractory Myeloma Patients Identifies an Enrichment of Mutations in Cereblon and MAPK Pathways
Authors: Elias Karl Mai, K. Martin Kortuem, Nur Hanafiah, Santiago Barrio, Laura Ann Bruins, Maximilian Merz, Jing Xu, Koenuel Pashayeva, Yuan-Xiao Zhu, Chang-Xin Shi, R. Alexander Stewart, Patrick Jedlowski, Mindaugas Andrulis, Anna Jauch, Jens Hillengass, Hartmut Goldschmidt, P. Leif Bergsagel, Esteban Braggio, A. Keith Stewart, Marc Steffen Raab
Journal: Blood (2015)
An Alternative Method for Myeloablative Allogenic Stem Cell Transplantation in Hematological Malignancies: Reduced BUCY2 and GCS-F Primed Bone Marrow Stem Cells
Authors: Eucario León-Rodríguez, Patricia Guzmán-Uribe, Sandra Ileana Pérez-Álvarez, Andrea Castro-Sánchez, Karla Adriana Espinosa-Bautista, Alejandra Armengol-Alonso, Elena Tuna-Aguilar, Yvette Neme-Yunes, Irma Espinosa-Poblano, Antonio Velázquez-González
Journal: Blood (2012)
Cytomegalovirus pp65 - Recombinant Protein: A New Antigen for Efficient Restimulation of CD4+ and CD8+ pp65-Specific T Lymphocytes.
Authors: Anne Richter, Patricia Marschall, Marie Mohn, Uwe Odenthal, Silke Gösling, Manuel Skopnik, Volker Nölle, Mario Assenmacher
Journal: Blood (2004)
The Utility of the New Instrument for Comprehensive Symptom Profile Assessment In Patients with Multiple Myeloma
Authors: Andrei A Novik, Tatyana I Ionova, Svetlana A Kalyadina, Denis A Fedorenko, Nikita E Mochkin, Kira A Kurbatova, Tatyana P Nikitina, Gary I Gorodokin
Journal: Blood (2010)
Comparison of Platelet Function and Bleeding in Thrombocytopenic Patients with Immune Thrombocytopenic Purpura (ITP) and Chemotherapy-Induced Thrombocytopenia (CIT).
Authors: Bethan Psaila, James B. Bussel, Matthew D. Linden, You Fu Li, Marc R. Barnard, Chinara M. Tate, Lemke K. Page, Eric J. Feldman, Andrew L. Frelinger, Alan D. Michelson
Journal: Blood (2007)
Polymorphisms in the Promoter Region of the Mcl-1 Gene Are Associated with Ex Vivo Cytotoxicity but Not with Response to Treatment in Advanced Chronic Lymphocytic Leukemia (CLL).
Authors: Neus Villamor, Silvia Marce, Francesc Bosch, Anna Ferrer, Patricia Pérez-Galán, Armando López-Guillermo, Eva Giné, Elías Campo, Emili Montserrat, Dolors Colomer
Journal: Blood (2005)
Association of “Weekend Warrior” and Other Leisure Time Physical Activity Patterns With Risks for All-Cause, Cardiovascular Disease, and Cancer Mortality
Authors: Gary O’Donovan, I-Min Lee, Mark Hamer, Emmanuel Stamatakis
Journal: JAMA Internal Medicine (2017)

<h3>Importance</h3> More research is required to clarify the association between physical activity and health in “weekend warriors” who perform all their exercise in 1 or 2 sessions per week. <h3>Objective</h3> To investigate associations between the weekend warrior and other physical activity patterns and the risks for all-cause, cardiovascular disease (CVD), and cancer mortality. <h3>Design, Setting, and Participants</h3> This pooled analysis of household-based surveillance studies included 11 cohorts of respondents to the Health Survey for England and Scottish Health Survey with prospective linkage to mortality records. Respondents 40 years or older were included in the analysis. Data were collected from 1994 to 2012 and analyzed in 2016. <h3>Exposures</h3> Self-reported leisure time physical activity, with activity patterns defined as inactive (reporting no moderate- or vigorous-intensity activities), insufficiently active (reporting &lt;150 min/wk in moderate-intensity and &lt;75 min/wk in vigorous-intensity activities), weekend warrior (reporting ≥150 min/wk in moderate-intensity or ≥75 min/wk in vigorous-intensity activities from 1 or 2 sessions), and regularly active (reporting ≥150 min/wk in moderate-intensity or ≥75 min/wk in vigorous-intensity activities from ≥3 sessions). The insufficiently active participants were also characterized by physical activity frequency. <h3>Main Outcomes and Measures</h3> All-cause, CVD, and cancer mortality ascertained from death certificates. <h3>Results</h3> Among the 63 591 adult respondents (45.9% male; 54.1% female; mean [SD] age, 58.6 [11.9] years), 8802 deaths from all causes, 2780 deaths from CVD, and 2526 from cancer occurred during 561 159 person-years of follow-up. Compared with the inactive participants, the hazard ratio (HR) for all-cause mortality was 0.66 (95% CI, 0.62-0.72) in insufficiently active participants who reported 1 to 2 sessions per week, 0.70 (95% CI, 0.60-0.82) in weekend warrior participants, and 0.65 (95% CI, 0.58-0.73) in regularly active participants. Compared with the inactive participants, the HR for CVD mortality was 0.60 (95% CI, 0.52-0.69) in insufficiently active participants who reported 1 or 2 sessions per week, 0.60 (95% CI, 0.45-0.82) in weekend warrior participants, and 0.59 (95% CI, 0.48-0.73) in regularly active participants. Compared with the inactive participants, the HR for cancer mortality was 0.83 (95% CI, 0.73-0.94) in insufficiently active participants who reported 1 or 2 sessions per week, 0.82 (95% CI, 0.63-1.06) in weekend warrior participants, and 0.79 (95% CI, 0.66-0.94) in regularly active participants. <h3>Conclusions and Relevance</h3> Weekend warrior and other leisure time physical activity patterns characterized by 1 or 2 sessions per week may be sufficient to reduce all-cause, CVD, and cancer mortality risks regardless of adherence to prevailing physical activity guidelines.
The combination of Index of NASH score and liver stiffness improves the noninvasive diagnostic accuracy for severe liver fibrosis in patients with NonAlcoholic Fatty Liver Disease.
Authors: R. Ibrahim, Chiara Rosso, S. Petta, M. Cucco, Milena Marietti, Gian Paolo Caviglia, Maria Lorena Abate, C. Cammà, Antonina Smedile, A. Craxì, Giorgio Maria Saracco, Elisabetta Bugianesi
Journal: Hepatology (2016)
Risk-Stratification Treatment Directed by Minimal Residual Disease Improves the Outcome of Acute Myeloid Leukemia with t(8;21) in First Complete Remission: Results of the AML05 Multicentre Trial
Authors: Honghu Zhu, Xiao-hui Zhang, Yazhen Qin, Hao Jiang, Dai-Hong Liu, Huan Chen, Qian Jiang, Jun-Yue Chai, Li-Ru Wang, Xiaojun Huang
Journal: Blood (2012)
Serum High Expression of Micorna-214 Is a Novel Predictor for Myeloma Bone Disease and Poor Prognosis
Authors: Mu Hao, Meirong Zang, Yan Xu, Yu Qin, Lei Zhao, Shuhui Deng, Weiwei Sui, Gang An, Zengjun Li, Yi Shuhua, Fenghuang Zhan, Lugui Qiu
Journal: Blood (2015)
Hybrid Imaging Labels: Providing the Link Between Mass Spectrometry-Based Molecular Pathology and Theranostics
Authors: Tessa Buckle, Steffen van der Wal, Stijn J.M. van Malderen, Larissa Müller, Joeri Kuil, Vincent van Unen, Ruud J.B. Peters, Margaretha E.M. van Bemmel, Liam A. McDonnell, Aldrik H. Velders, Frits Koning, Frank Vanhaeke, Fijs W. B. van Leeuwen
Journal: Theranostics (2017)

Background: Development of theranostic concepts that include inductively coupled plasma mass spectrometry (ICP-MS) and laser ablation ICP-MS (LA-ICP-MS) imaging can be hindered by the lack of a direct comparison to more standardly used methods for in vitro and in vivo evaluation; e.g.fluorescence or nuclear medicine.In this study a bimodal (or rather, hybrid) tracer that contains both a fluorescent dye and a chelate was used to evaluate the existence of a direct link between mass spectrometry (MS) and in vitro and in vivo molecular imaging findings using fluorescence and radioisotopes.At the same time, the hybrid label was used to determine whether the use of a single isotope label would allow for MS-based diagnostics.Methods: A hybrid label that contained both a DTPA chelate (that was coordinated with either 165 Ho or 111 In) and a Cy5 fluorescent dye was coupled to the chemokine receptor 4 (CXCR4) targeting peptide Ac-TZ14011 (hybrid-Cy5-Ac-TZ4011).This receptor targeting tracer was used to 1) validate the efficacy of ( 165 Ho-based) mass-cytometry in determining the receptor affinity via comparison with fluorescence-based flow cytometry (Cy5), 2) evaluate the microscopic binding pattern of the tracer in tumor cells using both fluorescence confocal imaging (Cy5) and LA-ICP-MS-imaging ( 165 Ho), 3) compare in vivo biodistribution patterns obtained with ICP-MS ( 165 Ho) and radiodetection ( 111 In) after intravenous administration of hybrid-Cy5-Ac-TZ4011 in tumor-bearing mice.Finally, LA-ICP-MS-imaging ( 165 Ho) was linked to fluorescence-based analysis of excised tissue samples (Cy5).Results: Analysis with both mass-cytometry and flow cytometry revealed a similar receptor affinity, respectively 352 ± 141 nM and 245 ± 65 nM (p = 0.08), but with a much lower detection sensitivity for the first modality.In vitro LA-ICP-MS imaging ( 165 Ho) enabled clear discrimination between CXCR4 positive and negative cells, but fluorescence microscopy was required to determine the intracellular distribution.In vivo biodistribution patterns obtained with ICP-MS ( 165 Ho) and radiodetection ( 111 In) of the hybrid peptide were shown to be similar.Assessment of tracer distribution in excised tissues revealed the location of tracer uptake with both LA-ICP-MS-imaging and fluorescence imaging.Ivyspring
The Role of Spleen Directed Therapy and Predictors of Outcomes with Reduced Intensity Conditioning Allogeneic Hematopoietic Stem Cell Transplantation for Patients with Primary Myelofibrosis and Splenomegaly
Authors: Daniel K. Partain, Moussab Damlaj, Aref Al-Kali, Naseema Gangat, Mark R Litzow, William J Hogan, Shahrukh K. Hashmi, Wilson I. Gonsalves, James M. Foran, Vivek Roy, Ruben A. Mesa, Jeanne Palmer, James L. Slack, Mrinal M Patnaik
Journal: Blood (2015)