AB0051 Regulatory T cells in rheumatoid arthritis, systemic lupus erythematosus and behcet’s disease
Authors: J.N. Chae, C.G. Jung, G. Choi, J.-S. Ha, S.-H. Park, Y.S. Kim, S.-H. Kim
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Treg cells play an important role in the maintenance of peripheral immunological self-tolerance through suppression of self-reactive T cells. Changes of the proportion of Treg cells within T cells as well as that of Treg subpopulations such as CD45RA<sup>+</sup>FoxP3<sup>lo</sup> resting Treg (rTreg) and CD45RA<sup>-</sup>FoxP3<sup>hi</sup> activated Treg (aTreg) in peripheral blood have been reported in several autoimmune diseases, but results have been variable. <h3>Objectives</h3> We investigated the proportion of Treg cell and its subpopulations in peripheral blood from patients with three autoimmune diseases, SLE, RA and BD. Then we intended to find possible relationships between changes of Treg cells and autoimmune mechanisms in such disease. <h3>Methods</h3> Peripheral blood samples were obtained from 42 rheumatoid arthritis (RA), 13 systemic lupus sclerosis (SLE), 7 Behcet's disease (BD) and 22 healthy controls. In flow cytometric analysis, CD4<sup>+</sup>CD25<sup>high</sup>FoxP3<sup>+</sup> population was analyzed as Treg cells. The CD4<sup>+</sup>CD25<sup>++</sup>CD45RA<sup>+</sup> and CD4<sup>+</sup>CD25<sup>+++</sup>CD45RA<sup>-</sup> cell populations were gated as rTreg and aTreg, respectively. <h3>Results</h3> The proportion of CD4<sup>+</sup>CD25<sup>high</sup>FoxP3<sup>+</sup> Treg was significantly lower in RA patients (<i>P</i>&lt;0.001) and BD patients (<i>P</i>&lt;0.01) compared to healthy controls. SLE patients also showed lower proportion of CD4<sup>+</sup>CD25<sup>high</sup>FoxP3<sup>+</sup> Treg cells than controls, but it was not statistically significant. The proportion of rTreg and non-suppressive T cells were similar among RA, SLE and BD patients and healthy controls. However, the proportion of aTreg was significantly lower in RA patients (<i>P</i>&lt;0.01) and BD patients (<i>P</i>&lt;0.05) compared to healthy controls. Comparison of the ratio of aTreg to rTreg revealed a significantly decreased ratio in RA patients compared to healthy controls (<i>P</i>&lt;0.01). SLE and BD patients did not show statistically significant changes of the ratio of aTreg to rTreg compared to controls. <h3>Conclusions</h3> The decrement of Treg population was significant in RA and BD patients than SLE, and these findings likely resulted from the decrement of the aTreg subpopulation fraction. Although a variety of studies has evaluated Treg in autoimmune diseases, we suggest that the distinction and evaluation of the proportions of Treg subpopulation, especially aTreg, would be more informative in assessing the dynamics of Treg cells as well as understanding the mechanism of autoimmune diseases. <h3>References</h3> Yagi H, Nomura T, Nakamura K, Yamazaki S, Kitawaki T, Hori S, Maeda M, Onodera M, Uchiyama T, Fujii S, Sakaguchi S (2004) Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells. Int Immunol 16(11): 1643-56 Miyara M, Yoshioka Y, Kitoh A, Shima T, Wing K, Niwa A, Parizot C, Taflin C, Heike T, Valeyre D, Mathian A, Nakahata T, Yamaguchi T, Nomura T, Ono M, Amoura Z, Gorochov G, Sakaguchi S (2009) Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor. Immunity 30(6): 899-911 Cvetanovich GL, Hafler DA (2010) Human regulatory T cells in autoimmune diseases. Curr Opin Immunol 22(6): 753-60 Kuhn A, Beissert S, Krammer PH (2009) CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res 301(1):71-81 <h3>Disclosure of Interest</h3> None Declared