tbuckley/cabot-search huggingface dataset:
Abstract 207: PTEN contributes to p53 activation during skin transformation
Authors: Delira F. Robbins, Xin Gu, Jianfeng Liu, Jacqulyne Ponville, Kerri Morris, Yunfeng Zhao
Journal: Cancer Research (2011)

Abstract Our previous studies have shown that the tumor suppressor p53 is activated and translocates to the nucleus, as well as, mitochondria during skin carcinogenesis. However, how p53, a short-lived protein is stabilized, and whether it works with other tumor suppressors remains unclear. We hypothesize that PTEN contributes to TPA-induced p53 activation in the early stages of skin carcinogenesis. Following TPA treatment, PTEN expression in the whole cell lysate was reduced; however, nuclear PTEN expression was increased, and the p53-PTEN interaction was detected via immunoprecipitation in murine skin epidermal JB6 P+ cells, an effect accompanied by an increase in the proapoptotic protein and p53 transcriptional target, Bax. In cell transfection experiments, knocking down PTEN expression via siRNA was sufficient to suppress p53 transcriptional activity as evidenced by decreased Bax expression. Similar effects were seen when p53 transcriptional activity was blocked by pifithrin-α, a specific p53 inhibitor that blocks p53 nuclear translocation. In addition, TPA-induced PTEN expression was significantly reduced in cells that were pre-treated with pifithrin-α. Our results indicate that the tumor suppressor, PTEN, promotes p53 activation in the early stages of TPA-mediated skin tumorigenesis to regulate the expression and transcriptional activity of p53. Therefore modulators of the PTEN- p53 complex can be therapeutically useful in the treatment of skin carcinogenesis and early-stage tumor promotion. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 207. doi:10.1158/1538-7445.AM2011-207
Reversal of Drug-Induced Rhabdomyolysis on Bone Scan
Authors: Joseph Abrams, Serafin Tiu
Journal: Clinical Nuclear Medicine (2011)

A 75-year-old man with prostate cancer was referred for metastatic workup. A Tc-99m methylene diphosphonate bone scan was performed which revealed diffusely increased radiopharmaceutical uptake in the muscles of the arms and thighs. The patient was taking simvastatin 80 mg per day and gemfibrozil 600 mg twice a day for high cholesterol. The patient reported myalgias, and laboratory evaluation was consistent with rhabdomyolysis. After discontinuation of the anticholesterol medications, the clinical and laboratory evaluations normalized. Bone scan performed 1 year later demonstrated complete resolution of muscle uptake.
Lack of Effect of Induction of Hypothermia after Acute Brain Injury
Authors: Guy L. Clifton, Emmy R. Miller, Sung C. Choi, Harvey S. Levin, Stephen McCauley, Kenneth R. Smith, J. Paul Muizelaar, Franklin C. Wagner, Donald W. Marion, Thomas G. Luerssen, Randall M. Chesnut, Michael Schwartz
Journal: New England Journal of Medicine (2001)

Induction of hypothermia in patients with brain injury was shown to improve outcomes in small clinical studies, but the results were not definitive. To study this issue, we conducted a multicenter trial comparing the effects of hypothermia with those of normothermia in patients with acute brain injury.
THU0375 Can Radial Speed of Sound Measurements Identify Osteoporosis at the Radius as Measured by DXA?
Authors: A. Oral, D. Sindel, P. Kursuz Koseoglu, A. Yaliman
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> One-third radius (33% radius) bone mineral density (BMD) measurements of the non-dominant forearm using dual-energy X-ray absorptiometry (DXA) may be used for the diagnosis of osteoporosis in certain circumstances. <h3>Objectives</h3> The aim of this study was to investigate the ability of radial speed of sound (SOS) measurements for identifying osteoporosis at the radius as measured by DXA. <h3>Methods</h3> Both 1/3 radius BMD and SOS of the non-dominant forearm were measured in 128 women aged between 21 and 79 years using DXA (Hologic QDR® 4000) and a multi-site quantitative ultrasound (QUS) device (Sunlight Omnisense™ 8000), respectively. Osteoporosis at the radius was defined according to the WHO criteria (a T-score ≤-2.5) in postmenopausal women in the sample studied. In premenopausal women, Z-scores of ≤-2.0 were used for defining osteoporosis or BMD below the expected range for age. <h3>Results</h3> Pearson correlation coefficients revealed statistically significant correlations between DXA 1/3 radius BMD and radial SOS measurements (r=0.496; p&lt;0.001) as well as 1/3 radius and radial SOS T-scores (r=0.495; p&lt;0.001). The areas under the receiver operating characteristic curves (AUCs) for radial SOS and its T-score for predicting osteoporosis or BMD below the expected range were 0.762 (p&lt;0.001) and 0.760 (p&lt;0.001), respectively. <h3>Conclusions</h3> Significant correlations between radial BMD measurements and QUS parameters and fair AUCs for QUS parameters for identifying osteoporosis or BMD below the expected range found in this small sample of women may have implications that radial SOS measurements may provide valuable information for treatment decisions when DXA cannot be done in any region of interest. <h3>Disclosure of Interest</h3> None Declared
1184 FRAGMENT BASED DRUG DISCOVERY OF NEW CYCLOPHILIN INHIBITORS UNRELATED TO CYCLOSPORINE A THAT POTENTLY INHIBIT HCV REPLICATION
Authors: A. Ahmed-Belkacem, L. Colliandre, N. Ahnou, P. Barthe, W. Bourget, D. Douguet, J.-F. Guichou, J.-M. Pawlotsky
Journal: Journal of Hepatology (2013)
THE CONTEMPORARY USE OF ANGIOGRAPHY AND REVASCULARIZATION AMONG PATIENTS WITH NON-ST SEGMENT ELEVATION MYOCARDIAL INFARCTION IN THE UNITED STATES VERSUS SOUTH KOREA
Authors: Hyun-Jae Kang, DaJuanicia Holmes, Tracy Wang, Karen Alexander, Myung Ho Jeong, Hyo-Soo Kim, Eric Bates, Timothy Henry, Eric Peterson, Matthew Roe
Journal: Journal of the American College of Cardiology (2014)
P840 ALTERATION IN LIPID METABOLISM AFTER AN ORAL FAT LOAD IN PATIENTS WITH NAFLD
Authors: L. Mezzabotta, E. Vanni, C. Rosso, M. Gaggini, R. Gambino, E. Buzzigoli, C. Saponaro, D. Ciociaro, M.L. Abate, F. Saba, F. Salomone, G.P. Caviglia, S. Carenzi, A. Smedile, M. Rizzetto, M. Cassader, A. Gastaldelli, E. Bugianesi
Journal: Journal of Hepatology (2014)
A Pattern of Blood-Volume Responses to Open-Heart Surgery
Authors: Robert L. Berger, Thomas F. Boyd, Phillip S. Marcus
Journal: New England Journal of Medicine (1964)

THE maintenance of an adequate circulating blood volume is one of the most important requirements in open-heart surgery. A reduction in the effective circulatory volume results in poor tissue perfusion and enhances the hypoxic metabolic acidosis of cardiopulmonary bypass. The combination of acidosis and hypovolemia may result in irreversible shock. Alternately, hypervolemia is equally hazardous, for a recently wounded and recuperating heart may not be able to cope with the burdens of an excessive circulating volume.The most reliable guide to the estimation of blood volume is its direct measurement. The conventional methods of estimations, such as clinical signs, pressure . . .
AB0800 Bone Mineral Density in Postmenopausal Women with Hip Osteoarthritis
Authors: N. Grygorieva, V. Povoroznyuk, S. Krochak
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Osteoporosis and osteoarthritis are the common diseases in women in postmenopausal period. The reason why osteoarthritis and osteoporosis do not affect all women during their postmenopausal period has not been ascertained yet, the mechanisms of bone loss and cartilage degeneration when ageing have not been studied completely. Some literature data confirmed that indices of X-ray absorptiometry in women with hip osteoarthritis (HOA) are higher, but other did not confirm this point. <h3>Objectives</h3> The aim was to study the particularity of relationship between state of bone and development of HOA in postmenopausal women. <h3>Methods</h3> 69 women in postmenopausal period aged 50-79 years old with hip osteoarthritis were examined (mean age - 65.6±0.6 years old, weight - 74.1±0.8 kg, height – 158.6±0.4 cm, age of menopause - 49.2±0.4 years, duration of postmenopausal period - 16.2±0.6 years). The control group consisted the 60 healthy women without HOA. A diagnosis of osteoarthritis was performed due to the ACR criteria9s. Women with primary knee osteoarthritis were excluded from the study. Bone state was measured by Dual-energy X-ray absorptiometry "Prodigy"(DXA) and calcaneus9s quantitative ultrasound (QUS) densitometry "Sahara". <h3>Results</h3> We found the significant correlation between indices of DXA and ultrasound densitometry and presence and stage of HOA in postmenopausal women. In patients with HOA data of X-ray absorptiometry were significantly higher compared with healthy women (bone mineral density (BMD) of Trunk: F=7.71, p=0.006; Spine: F=6.32, p=0.01; Neck: F=4.08, p=0.04; Wards area: F=4.69, p=0.03 and Total BMD: F=5.61, p=0.02). Indices of ultrasound densitometry in women with HOA also were significantly higher compared with healthy women (Est BMD: F=6.49, p=0.01; BUA: F=9.8, p=0.002; SOS: F=3.45, p=0.05). In postmenopausal women we did not found significant relationship between some indices of DXA (L1-L4, total hip and radius) and presence of hip osteoarthritis. <h3>Conclusions</h3> Presence and stage of HOA had significantly influence on ultrasound densitometry and DXA indices. It is important in assessment of bone state in women in postmenopausal period with hip osteoarthritis. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4569
A community-based public-academic liaison program
Authors: A B Santos, J C Ballenger, J J Bevilacqua, J J Zealberg, T G Hiers, S McLeod-Bryant, P A Deci, L J Rames
Journal: American Journal of Psychiatry (1994)

The authors discuss obstacles and incentives associated with successful community-based public-academic liaison activities and illustrate their conclusions by describing their public-academic liaison program, which received the American College of Psychiatrists' first annual Award for Creativity in Psychiatric Education.The first 8 years of a state/university collaboration are described in which the parties involved first developed a variety of innovative services designed to fill specific gaps in the public service delivery system and subsequently integrated academic research and training components.The carefully planned and monitored process resulted in the exponential growth of interest in public sector work, the realization of the university's primary goals of developing high quality training and research sites, a substantial increase in the number of graduates accepting positions in the public sector, several projects funded by the National Institute of Mental Health, and two national awards.These collaborations illustrate the highly complementary relationship of public-academic liaison activities and their potential capacity to improve access to services, substantially improve the quality of these services, generate extramural support for services research, and increase the number of well-trained professionals in the public sector.
Prognosis of Stage I Lymphosarcoma and Reticulum-Cell Sarcoma
Authors: Allan Lipton, Burton J. Lee
Journal: New England Journal of Medicine (1971)

Eleven patients with Stage I (i.e., negative lymphangiography) lymphosarcoma and 15 with Stage I reticulum-cell sarcoma were followed for at least two years. There were three deaths and six recurrences, all within 32 months of diagnosis. Twenty-four of 26 patients (92 per cent) were alive at two years, and 17 of 20 (85 per cent) at four years. Early bone-marrow invasion was seen only in the three patients who died. Six patients survived for a mean of over 111 months with nodal and extranodal recurrences. There were two deaths and six recurrences in 19 patients (42 per cent) receiving less than 3500 R (supervoltage in most cases) and only one death and no recurrences in seven patients (14 per cent) receiving at least 3500 R to the primary tumor site.
Abstract 796: Utilizing TCRβ and whole exome sequencing to determine the clonality and mutational spectrum of p53-null thymic lymphomas.
Authors: Crissy Dudgeon, Chang Chan, Yvonne Sun, Arnold J. Levine
Journal: Cancer Research (2013)

Abstract The tumor suppressor p53 is nonfunctional in over 50% of cancers. The ability for p53 to protect the cell from tumorigenesis is rooted in its ability to induce cell cycle arrest, apoptosis, and senescence following genotoxic stress. Furthermore, loss of p53 in mice gives rise to spontaneous thymic lymphomas and sarcomas. In this study, we used the p53-KO mouse as a model for progression of tumorigenesis. We first analyzed genomic DNA of p53-KO and WT normal thymus at various ages using TCRβ sequencing. This procedure allowed us to determine the clonality of the T cell repertoire. We then analyzed the clonality of p53-KO thymic lymphomas. Our results indicated that while the highest T cell clone was 0.08% for normal thymus, the highest T cell clone for the p53-KO tumor was 70%, indicating that the p53-KO thymic lymphomas were monoclonal. Building on this data, we analyzed the mutations occurring in the thymic lymphomas by mouse exome sequencing. We believe that combining TCRβ sequencing with mouse exome sequencing will allow for a better understanding of the clonality and genetic mutations required for cellular transformation. Citation Format: Crissy Dudgeon, Chang Chan, Yvonne Sun, Arnold J. Levine. Utilizing TCRβ and whole exome sequencing to determine the clonality and mutational spectrum of p53-null thymic lymphomas. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 796. doi:10.1158/1538-7445.AM2013-796
Handle Survivors with Care
Authors: Armand Sprecher
Journal: New England Journal of Medicine (2015)

In 1967, a woman became ill after exposure to a newly discovered pathogen that we now call Marburg virus, a member of the family Filoviridae (filoviruses), to which Ebola virus also belongs.1 Testing of the semen of her husband, who had recovered from the disease 6 weeks previously, determined that her exposure was through sexual intercourse. This was the first confirmed case of sexual transmission of filovirus disease from a convalescent man. It was also the last . . . until the West African outbreak. In March 2015, Ebola virus disease (EVD) developed in a Liberian woman after the country . . .
Brief Communication: Colon Carcinoma of Highly Inbred Rats
Authors: Makoto Miyamoto, Shoichi Takizawa
Journal: JNCI Journal of the National Cancer Institute (1975)

Colon carcinoma in related WF rats was found in several colonies. The tumors were noted in generations 12-15. Usually, the lesions were detected in 6-month-old animals. However, in later generations, the tumors seemed to occur earlier.
A Subcutaneous Delta-Positive T-Cell Lymphoma That Produces Interferon Gamma
Authors: Günter Burg, Reinhard Dummer, Martin Wilhelm, Frank Nestle, Maria Michaela Ott, Alfred Feller, Hiltrud Hefner, Ute Lanz, Andreas Schwinn, Johannes Wiede
Journal: New England Journal of Medicine (1991)

WE report an unusual form of cutaneous T-cell lymphoma characterized by subcutaneous tumors that regress spontaneously, fever, progressive leukopenia, high levels of interferon gamma in the serum, and an increased natural-killer-cell activity of peripheral-blood mononuclear cells. Histologically, the infiltrate consists of nonepidermotropic, pleomorphic lymphoid cells located in the subcutaneous fatty tissue. These cells express the phenotype of immature (CD1+, CD3+, CD4-, CD5-, CD8-), activated (CD30+, CD25+, HLA-DR+) T cells positive for delta T-cell receptors. They express the natural-killer-cell phenotype (CD56+) and produce interferon gamma in cell culture. Case Report A 70-year-old woman presented in March 1985 with recurrent, spontaneously regressing, . . .
SAT0116 What Drives the RAPID Increase in Risk of Acute Coronary Syndrome following RA Diagnosis?
Authors: Ä. Mantel, M. Holmqvist, F. Nyberg, G. Tornling, T. Frisell, L. Alfredsson, J. Askling
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> The risk of cardiovascular disease (CVD) is increased in RA patients. We have previously reported a rapid increase in risk of acute coronary syndromes (ACS) following RA diagnosis. Despite known variation in time-to-risk patterns for different subtypes of CVD, indicating differences in underlying mechanisms, most studies on CV risk factors in RA so far have focused on composite CVD-outcomes. <h3>Objectives</h3> To investigate risk factors specifically for ACS in patients with new-onset RA. <h3>Methods</h3> We performed a case-control study nested in a cohort of patients with incident RA included in the Epidemiological Investigation of Rheumatoid Arthritis (EIRA) study. Cases with ACS, defined as a hospitalization or cause-of-death listing ACS following RA diagnosis, were identified in the national Patient Register and Cause of Death Register. Up to 5 controls, free of ACS, per case were selected from the same RA cohort matched on sex, year of RA diagnosis and EIRA center. Information on exposures of interest was collected as follows: I) Serology (antibodies towards citrullinated peptides [ACPA], rheumatoid factor [RF]) and shared epitope alleles (SE) was retrieved from the EIRA-study. II) Clinical disease activity data (DAS-28 and its components [CRP, ESR, Joint status, Global Health [GH] patient assessment] were abstracted from medical charts and supplemented with information from Swedish Rheumatology Quality Register (SRQ). III) Information on other risk factors and pharmacotherapies was collected from medical charts, from the Patient Register, and from the national Prescribed Drug Register. IV) Net days of sick leave and disability pension during the first year following RA-diagnosis was obtained from the social insurance agency. Area under the curve (AUC) measures of disease activity per year and for complete follow-up following RA diagnosis was estimated by taking the average of all reported values per time-period. Odds ratios were adjusted for matching factors and age. <h3>Results</h3> 93 cases and 440 controls with information were included. Baseline smoking, high BMI and history of MI, as well as high clinical disease activity (AUC of ESR, DAS28 and GH) and functional measures (net days of sick leave) during the first year after RA onset were all associated with ACS risk; by contrast, neither treatment (methotrexate or other DMARDs), nor serology (RF/ACPA), nor SE were associated with ACS. The above pattern remained when AUC measures during complete follow-up (average 5.3 yrs) were assessed. <h3>Conclusions</h3> Disease activity and perceived health already during the first year following RA onset are strong drivers of ACS risk whereas RF, ACPA and SE are not. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3565
Abstract 1837: Exosomal non-coding RNAs as mediators for cell-cell communications .
Authors: Ramesh Singh, Yin-Yuan Mo
Journal: Cancer Research (2013)

Abstract Introduction: Exosomes are 30-100nm membrane vesicles of endocytic origin containing proteins, mRNAs, microRNAs and lincRNAs. MicroRNAs are small and lincRNAs are large intergenic non-coding RNAs. They are emerging as key regulators of diverse cellular processes including cancer. In this study, we investigated the exosome-mediated transfer of microRNAs and lincRNAs, regulation and functional significance in breast cancer. Approach: Exosomes were isolated by ultracentrifugation and stained with PKH26 dye for cellular uptake in different cells by confocal microscopy. This uptake was also shown by exosome isolated from stable 293T cell line overexpressing the pCDH-CD63-GFP fusion protein. To determine the effect of nSMase2 on exosome secretion, stable cell lines were established with smpd3 gene. Levels of microRNAs and lincRNAs secreted in media were determined by real time PCR and their function validated through western blot and cell invasion. Results: We first showed that exosomes can be transferred among different cell lines through direct uptake. We found that miR-10b is highly expressed in metastatic breast cancer MDA-MB-231 cells as compared to non-metastatic breast cells. Also, nSMase2 promoted the exosome-mediated miR-10b secretion whereas ceramide inhibitor suppressed this secretion. Importantly, western blot revealed that upon uptake, miR-10b can still reduce the protein level of its target genes. We also found lincRNA 21A and BC200 to be secreted through exosome in cell type dependent manner with functional significance. Conclusion: We found that the exosomes secreted from cancer cells containing microRNA and lincRNA can be taken up by other cells and have functional role in the recipient cells suggesting the importance of exosome-mediated RNA transfer in tumor microenvironment. Citation Format: Ramesh Singh, Yin-Yuan Mo. Exosomal non-coding RNAs as mediators for cell-cell communications . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1837. doi:10.1158/1538-7445.AM2013-1837
OCULAR SARCOIDOSIS IS ASSOCIATED WITH ENDOTHELIAL DYSFUNCTION AND ARTERIAL STIFFNESS IN SARCOIDOSIS PATIENTS
Authors: Gerasimos Siasos, Theodore Paraskevopoulos, Elias Gialafos, Evangelos Oikonomou, Georgia Siasou, Nikolaos Gouliopoulos, Marina Zaromitidou, Konstantinos Mourouzis, Sotirios Tsalamandris, Charalambos Vlachopoulos, Eleni Kokkou, Christodoulos I Stefanadis, Dimitris Tousoulis
Journal: Journal of the American College of Cardiology (2015)