tbuckley/cabot-search huggingface dataset:
Abstract 250: Growth characteristics of breast cancer stem cells <i>in vitro</i> and <i>in vivo</i>.
Authors: Carnella M. Lee, Anandi Sawant, Ha-Ram Cha, Selvarangan Ponnazhagan
Journal: Cancer Research (2013)

Abstract Breast cancer (BCa) is the most common malignancy in United States women, accounting for greater than 40,000 deaths each year. Studies have attributed the presence of cancer stem cells (CSC) with resistance to chemotherapy as a key component of refractories of treatments in BCa. Breast cancer stem cells (BCa-SC) are characterized by CD24lo and CD44hi phenotype with mesenchymal properties, which differ from the epithelial CD24hi and CD44hi phenotype expressed in the majority of BCa cells. Thus, further understanding properties of BCa-SC may lead to the development of novel treatment options towards treating refractory cancer cells. Fundamental to this, further characterization of BCa-SC will be needed to identify therapeutic targets based CSC biology. To this end, we sought to characterize the plasticity of BCa-SC based on cell cycle in vitro and growth kinetics in vivo. A murine BCa cell line, 4T1, was cultured in CSC medium and analyzed every 6 hrs for CD24 and CD44 expression by flow cytometry. Results of this analysis indicated that specific BCa-SC markers peaked every 24 hours. This data supports a possible reprogramming of BCa-SC based on cell cycle regulation, suggesting the use of cell cycle inhibitors to block cells in particular stages of the cell cycle, which may improve therapeutic approaches to induce cell death. The stemness of these cells was further confirmed by real-time PCR. BCa-SC showed increased expression of ALDH1, TWIST, and TGF-β3 with decreased E-Cadherin and CD24 levels correlating to the phenotypic changes observed with CD24 and CD44 expression. To further examine the role of BCa-SC in tumor growth in vivo, BALB/c mice were subcutaneously implanted with populations of 4T1 cells that constituted CSC and non- cancer stem cells (NCSC) in different combinations and tumor growth was analyzed. Results showed that the growth kinetics CSC and NCSC when used alone was significantly lower in vivo compared to mixed populations containing both CSC and NCSC, suggesting CSC may attribute for the repopulation of the disease but an aggressive growth requires differentiated non-stem cells in the tumor microenvironment. Citation Format: Carnella M. Lee, Anandi Sawant, Ha-Ram Cha, Selvarangan Ponnazhagan. Growth characteristics of breast cancer stem cells in vitro and in vivo. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 250. doi:10.1158/1538-7445.AM2013-250
SP0140 Outcome Measures in Rheumatology (OMERACT): Status on the Flare Assessment Work
Authors: S.J. Bartlett
Journal: Annals of the Rheumatic Diseases (2014)

<h3></h3> Since 2006, the OMERACT RA Flare Group has been working together to define and measure RA flare to facilitate research and improve clinical practice. We are an international collaboration of rheumatology researchers, clinicians, patients, measurement specialists, and industry partners. Using multiple strategies including literature reviews, patient focus groups, patient and provider Delphi exercises, data from observational studies and consensus voting at OMERACT conferences, we developed a consensus-based definition for serious inflammatory RA flares and identified relevant domains to measure. This lecture will provide an overview of our recent work and present initial findings from ongoing efforts to validate an initial set of items for an RA Flare tool that can precisely and reliably measure flare severity across patients, providers and settings. Items provide an expanded assessment of disease worsening (beyond the traditional RA core set) from the patient9s perspective. Item responses from several large clinical and observational trials in North American and Europe are combined with clinical data to help identify, characterize and quantify RA flares from multiple perspectives and across relevant domains. Both traditional and modern psychometric approaches are being used to refine the final OMERACT RA Flare Questionnaire prior to release for widespread use. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.6245
Abstract 4201: AP1 factor inactivation in suprabasal epidermis causes increased epidermal hyperproliferation and hyperkeratosis but reduced carcinogen-dependent tumor formation
Authors: Ellen A. Rorke, Ralph Jans, Gautam Adhikary, David Berman, Richard L. Eckert
Journal: Cancer Research (2010)

Abstract AP1 (jun/fos) factors comprise a family of transcriptional regulators (c-jun, junB, junD, c-fos, FosB, Fra-1 and Fra-2) that are key controllers of epidermal keratinocyte survival and differentiation, and are important drivers of cancer development. Previous studies indicate that targeted inactivation of epidermal basal layer AP1 factor function by expression of dominant-negative c-jun (TAM67) in the basal epidermis produces no overt phenotypic impact, but does inhibit UVB and carcinogen-dependent epidermal tumor formation. We hypothesized that the role of AP1 factors is likely to be different in basal versus suprabasal epidermis. To test this hypothesis, we targeted TAM67 to the suprabasal epidermis using a tetracycline-inducible construct. Induction of suprabasal TAM67 expression results in marked epidermal hyperproliferation and hyperkeratosis. This is characterized by expression of basal layer and hyperproliferation-associated keratins in the suprabasal epidermis, suggesting that the cells are undergoing incomplete differentiation. We further demonstrate global changes in the AP1 factor signaling including changes in level and subcellular distribution of selected AP1 factors. Surprisingly, these animals display reduced susceptibility to carcinogen-dependent tumor induction. These novel observations, which suggest that perturbing AP1 factor function in the basal versus suprabasal layer produces different biological outcomes, is cancer relevant and points to the complexity of AP1-associated regulation and that fact that AP1 factor function differs as the tissue differentiates. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4201.
P660 THE EFFECT OH HEPATITIS B VIRUS INFECTION ON STEATOSIS IN HEPATITIS C VIRUS COINFECTED PATIENTS: THE BOSTIC STUDY
Authors: N. Goossens, N. Coppola, R. Zampino, M. Stanzione, H. Wedemeyer, F. Barrera, B. Müllhaupt, D. Moradpour, D. Semela, N. Semmo, R. Malinverni, M. Heim, J. George, M. Manns, L.E. Adinolfi, L. Rubbia-Brandt, F. Negro
Journal: Journal of Hepatology (2014)
Normal Vasculature in Tc-99m RBC Labeled Liver SPECT With Corresponding CT:
Authors: ORHAN ÖZ, WONSICK CHOE, LEISA BORDERS, REBHI AWAD
Journal: Clinical Nuclear Medicine (1996)

Hepatic tumors are often observed as “hot spots” on Tc-99m labeled RBC SPECT imaging. Because normal blood vessels also show increased activity, it would be important to know their location and appearance. The authors show contrast enhanced CT images with corresponding SPECT images of a patient who does not have a hepatic tumor. Additional SPECT images with different reconstruction processing are also shown to emphasize the importance of optimal processing.
Intense FDG Activity in a Case of Xanthogranulomatous Cholecystitis Without Elevated Fluorothymidine Activity
Authors: Kuruva Manohar, Bhagwant Rai Mittal, Anish Bhattacharya, Bishan Dass Radotra, Ganga Ram Verma
Journal: Clinical Nuclear Medicine (2012)

F-FDG PET/CT is a useful imaging modality to detect malignant gall bladder lesions. We present an image of a patient with a gall bladder mass that was found to be FDG avid but negative on F-fluorothymidine PET/CT imaging. This was later confirmed to be a case of xanthogranulomatous cholecystitis.
NCI Foreign Agreement Becoming More Common
Authors: J. Mathews
Journal: JNCI Journal of the National Cancer Institute (1992)

Journal Article NCI Foreign Agreement Becoming More Common Get access James Mathews James Mathews Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 84, Issue 17, 2 September 1992, Pages 1312–1313, https://doi.org/10.1093/jnci/84.17.1312 Published: 02 September 1992
AB1104 Hypovitaminosis D in female patients with myofasial pain syndrome
Authors: N. Kesiktas, M. Koser, N. Paker, D. Bugdayci, D. Sindel
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Vitamin D insufficiency has emerged as a pandemic health issue in the recent times. Recent observations and hypotheses suggest that VDI may affect almost every system of the body. A number of observations have suggested an association between low serum levels of vitamin D and higher incidence of chronic pain [1]. <h3>Objectives</h3> The present study was, carried out to examine hypovitaminosis D and its determinants in female patients with myofascial pain syndrome. <h3>Methods</h3> One hundred-sixty consecutive women with myofascial pain were included the study. For exclusion of patients with rickets and post menopausal osteoporosis, only the patients aged between 20-30 years were accepted to study. Patients with celiac disease, malabsorbtion symdrome, abnormal hepatic and renal function tests and patients taking medications known to affect vitamin D level were excluded. Informed consents were obtain from all patients. As part of their routine assessment, we recorded their vitamin D level (25-hydroxilated vitamin D), PTH, calsium, phosphor and other biocheimstry assays and pain characteristics with VAS. A risk factors list associated with hypovitaminosis like sun exposure (outfit), diet, pregnancy were applied. Logistic regression analysis were used in statistical evaluation with SPSS. <h3>Results</h3> There is a significant correlation with vitamin D and visual analogue scale of pain. Logistic regression analysis were showed that outfit was a significant factor for low level vitamin D. Myofascial pain patients were divided into two groups, depending on their outfitting style. In group-1, plasma 25 (OH)2 vitamin D3 and body mass index (BMI) was statistically significantly lower (p&lt;0.01). When the laboratory parameters were compared, There was no significant difference routine blood analyses. There were no difference between number of pregnancies and nutrition habbits between groups. <h3>Conclusions</h3> There is no consensus on the even optimal levels of vitamin D. Vitamin D insufficiency constitutes an unrecognized epidemic in many populations worldwide. Therefore, the causal association of any disease with vitamin D insufficiency or deficiency should be judged cautiously. Vitamin D might help pain therapy in myofascial pain syndromes. But at least it must be examined. <h3>References</h3> Straube S, Andrew Moore R, Derry S, McQuay HJ (2009) Vitamin D and chronic pain. Pain 141(1–2):10–13 <h3>Disclosure of Interest</h3> None Declared
AB0804 Experience of educational programs for physicians in diagnosis and treatment of osteoporosis in kazan (russia)
Authors: L. Myasoutova, A. Vasiliev, S. Lapshina
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Osteoporosis is a serious and badly diagnosed problem for doctors in various specialties. <h3>Objectives</h3> To evaluate the effectiveness of educational activities for general practitioners (GP), internists, neurologists in osteoporosis (OP) detection. <h3>Methods</h3> The educational schools are conducted to identify possible symptoms (nature of back pain, changes in posture and growth), risk factors for OP diagnosis, indications for bone densitometry, a complex of therapeutic measures for primary care physicians in Kazan since January 2011. Evaluation of the results was carried out according to analysis of outpatient rheumatological report from Rhematological Center (Kazan) in 2010 (base year) and 2011-2012 (after schools). <h3>Results</h3> After schools (2011-2012) significantly increased the number of patients (3.5-fold) with OP diagnosis verified by rheumatologist at a reception to 353 (primary OP-170 (48.2%), secondary OP - 183 (51.8%)) in end of 2011 and 581 (primary OP-292 (50.2%), secondary OP - 289 (49.8%)) - in 2012 compared to the base year 2010 (158: 19 (12%) - the primary OP 139 (88%) - the secondary OA). In addition, the number of patients with OP, who applied for the first time in the center of Kazan Rheumatology, increased from 118 (77.1%) in 2010 to 290 (82.1%) people in 2011 and 478 (82, 2%) people in 2012. In 2010 patients with primary OP were referred by physicians to a rheumatologist with other diagnoses in 76.2% of cases. In 2011, the percentage difference of primary diagnoses decreased to 37,9%, in 2012– to 21,3%. <h3>Conclusions</h3> Educational programs for primary care physicians are essential and contribute to early diagnosis of OP, timely administration of therapy, improve the quality of life of patients. The experience of the educational activities in Kazan has improved detection of OP by primary care physicians and timely referral to the center of the diagnosis and treatment of OP. <h3>Disclosure of Interest</h3> None Declared
PREVALENCE AND PREDICTORS OF SLEEP-DISORDERED BREATHING IN PATIENTS WITH STABLE CHRONIC HEART FAILURE: FINAL DATA OF THE SCHLAHF REGISTRY
Authors: Holger Woehrle, Michael Arzt, Olaf Oldenburg, Andrea Graml, Anna Drabik, Helmut Teschler, Erland Erdmann, Karl Wegscheider
Journal: Journal of the American College of Cardiology (2015)
Novel germ line DDX41 mutations define families with a lower age of MDS/AML onset and lymphoid malignancies
Authors: Maya Lewinsohn, Anna L. Brown, Luke M. Weinel, Connie Phung, George Rafidi, Ming K. Lee, Andreas W. Schreiber, Jinghua Feng, Milena Babic, Chan-Eng Chong, Young Lee, Agnes Yong, Graeme K. Suthers, Nicola Poplawski, Meryl Altree, Kerry Phillips, Louise Jaensch, Miriam Fine, Richard J. D’Andrea, Ian D. Lewis, Bruno C. Medeiros, Daniel A. Pollyea, Mary-Claire King, Tom Walsh, Siobán Keel, Akiko Shimamura, Lucy A. Godley, Christopher N. Hahn, Jane E. Churpek, Hamish S. Scott
Journal: Blood (2015)
Abstract 1657: Targeting Cullin-RING ligases by MLN4924 induces autophagy via modulating the HIF1-REDD1-TSC1-mTORC1-DEPTOR axis in breast cancer cells.
Authors: Yongchao Zhao, Xiufang Xiong, Lijun Jia, Yi Sun
Journal: Cancer Research (2013)

Abstract MLN4924, a newly discovered small molecule inhibitor of NEDD8-Activating Enzyme (NAE), inactivates Cullin-RING E3 ubiquitin ligases (CRLs) by blocking cullin neddylation. As a result, MLN4924 causes accumulation of several key substrates of CRLs and effectively suppresses tumor cell growth by inducing apoptosis and senescence. However, the role of MLN4924 in induction of autophagy and its biological significance are totally unknown. Here we showed that MLN4924 effectively induces autophagy in both time- and dose-dependent manners in breast cancer cells. Mechanistically, by inactivating CRLs, MLN4924 causes accumulation of DEPTOR and HIF1α. The siRNA knockdown and gene KO studies showed that DEPTOR and the HIF1α-REDD1-TSC1 axis are responsible for MLN4924-induced autophagy via inhibiting mTORC1. Biologically, autophagy is a survival signal to tumor cells, and blockage of autophagy via siRNA knockdown, gene KO and small molecule inhibitor remarkably enhanced MLN4924-induced apoptosis. Our study reveals an uncharacterized mechanism of MLN4924 action and provides the proof-of-concept evidence for strategic drug combination of MLN4924 with an autophagy inhibitor for maximal killing of tumor cells via enhancing apoptosis. Citation Format: Yongchao Zhao, Xiufang Xiong, Lijun Jia, Yi Sun. Targeting Cullin-RING ligases by MLN4924 induces autophagy via modulating the HIF1-REDD1-TSC1-mTORC1-DEPTOR axis in breast cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1657. doi:10.1158/1538-7445.AM2013-1657
EARLY VERSUS DELAYED USE OF BETA-BLOCKERS IN ACUTE CORONARY SYNDROMES: A LANDMARK ANALYSIS. FROM THE ISACS-TC STUDY
Authors: Beatrice Ricci, Edina Cenko, Zorana Vasiljevic, Maria Dorobantu, Sasko Kedev, Oliver Kalpak, Olivija Gustiene, Dijana Trininic, Božidarka Kneževic, Davor Miličić, Olivia Manfrini, Akos Koller, Lina Badimon, Raffaele Bugiardini
Journal: Journal of the American College of Cardiology (2015)
A1.29 In rheumatoid arthritis, smoking is not primarily associated with anti-citrullinaged protein antibodies, but with the presence of several autoantibodies
Authors: Ammar Muhammad, Tineke van Wesemael, Yuta Kochi, Maria D Mjaavatten, Kirsten Wevers-de Boer, Cornelia F Allaart, Leendert A Trouw, Akari Suzuki, Kazuhiko Yamamoto, Annette H M van der Helm-van Mil, Tom W J Huizinga, René E M Toes, Diane van der Woude
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background and Objectives</h3> In rheumatoid arthritis (RA), smoking has been described to be specifically associated with the presence of anti-citrullinated protein antibodies (ACPA). However, smoking has also been shown to be associated with the presence of autoantibodies in various other autoimmune diseases, such anti-dsDNA in systemic lupus erythematosus and anti-Jo1 in idiopathic inflammatory myopathy. We therefore investigated whether smoking is specifically associated with ACPA-positive RA, or with autoantibody-positive RA in general. <h3>Materials and Methods</h3> A meta-analysis was performed using RA patients from 5 countries: Norway, the Netherlands, Japan, Sweden, and the United Kingdom. Complete data on rheumatoid factor (RF)-, ACPA-status and tobacco exposure were available for 6320 RA patients. The odds ratios (ORs) and 95% confidence intervals (95% CIs) associated with the presence of RF, ACPA or both, were calculated by logistic regression comparing ever smokers with never smokers, and using the RF-negative ACPA-negative RA patients as the reference category. <h3>Results</h3> There was no significant association between smoking and RA in patients who were positive for only one antibody, being either RF (OR 1.04, 0.76 – 1.42) or ACPA (OR 1.00, 0.82 – 1.22). However, smoking was significantly associated with double-positive (RF-positive and ACPA-positive) RA (OR 1.55, 1.20 – 2.00). When ANA-status was also taken into account in the Dutch cohort, the association with smoking was strongest for the triple-positive group (OR = 2.43, 95% CI 1.47 – 4.00), although the difference with the double-positive RA patients (RF- and ACPA-positive, ANA-negative) (OR = 1.73, 95% CI 1.14 – 2.62) was not statistically significant. <h3>Conclusions</h3> Smoking is not specifically associated with ACPA-positive RA, but rather with the concurrent presence of RF and ACPA in RA patients. These data indicate that smoking predisposes to the development of several autoantibodies, and that current hypotheses about the role of smoking in the pathophysiology of RA may need to be revisited.
Endoscopic Ultrasonography in staging of upper gastrointestinal malignance with linear array transducer
Authors: RA Zaydan
Journal: Endoscopy (2006)

Background and aim of study: Since the echo layers of gastrointestinal wall are corresponding to the histological layers, EUS is the most accurate method for locoregional staging of upper gastrointestinal malignancy, so the aim of this study is to evaluate the accuracy of EUS in staging of upper gastrointestinal tract malignancy using curved array transducer. Patients and methods: This study include 74 patients with upper gastrointestinal malignancy (esophageal (19), gastric (32), pancreatic (14), ampullary (9) referred to the gastroenterology and hepatology teaching hospital between April 2002 and March 2004 were submitted to the EUS examination by linear array echoendoscope to assess the accuracy of EUS staging of these malignancies before surgical/pathological staging. Results: In the leuminal malignancy (esophageal and gastric) the result between EUS staging and surgical/pathological staging were similar. Although EUS is highly accurate for T staging of locoregionaly advance disease, but is far less accurate in T staging of locoregionaly limited disease. In pancreatic malignancy (14 patients), EUS (T) staging was compatible with that of surgical/pathological staging. However, EUS is highly accurate for detecting the invasion of major vessels (portal and splenic) by tumors. Regarding the ampullary tumors (9 patients), EUS is accurate in T staging, and it is safe, impact on management of ampullary tumors. Conclusion: EUS is highly accurate in of upper gastrointestinal tract malignancy particularly in advance tumors.
Endoscopic closure of esophageal fistula using a novel ”clips and loop” method
Authors: C. Luigiano, F. Ferrara, A. Polifemo, C. Fabbri, S. Ghersi, M. Bassi, N. D’Imperio
Journal: Endoscopy (2009)

Fistula can develop rarely between the esophageal lumen and other mediastinal structures. The majority of cases are caused by malignancy. Benign causes include infections, other inflammatory conditions, postsurgical trauma, and prolonged periods of endotracheal intubation or tracheostomy tube placement. In most cases of fistula, surgery is required. There are, however, a number of reports in the literature of endoscopic methods of attempting fistula closure: the application of fibrin glue, use of covered esophageal stents, and clipping have been described, for closure of esophagotracheal, bronchoesophageal, and esophagopleural fistulas [1] [2] [3] [4].
Abstract 5599: Screening for large genomic rearrangements in the FANCA and FANCJ genes reveals extensive genomic FANCA deletion in a Finnish breast cancer family
Authors: Szilvia Solyom, Robert Winqvist, Jenni Nikkilä, Katri Pylkäs
Journal: Cancer Research (2011)

Abstract A portion of familial breast cancer is accounted for by mutations in the same genes that are inactivated in Fanconi anemia (FA). In the current study we set out to evaluate the role of large genomic rearrangements in the FANCA and FANCJ/BRIP1 genes in Finnish breast cancer families. Mutations in the upstream FANCA gene account for the majority of FA patients, and about one-third of all FANCA mutations are large intragenic deletions. Based on the gene's hypermutable nature, the protein's interaction with BRCA1 and its involvement in genomic integrity maintenance, we considered FANCA as a candidate for breast cancer susceptibility. In FANCJ, which is a downstream FA and known breast cancer susceptibility gene, early truncating mutations have been reported, but no large deletions have been recognized so far. For the present study, blood DNA from affected index persons of 100 Northern Finnish breast cancer families were assessed for possible constitutional exonic deletions or amplifications in FANCA and FANCJ by using the multiplex ligation-dependent probe amplification (MLPA) method. We identified a novel large heterozygous FANCA deletion in one of the studied breast cancer families, removing the promoter region and the first 12 exons of the gene. The defective allele was absent from all of the tested 124 controls as well as 540 unselected breast cancer cases, but was present in the father of the index patient having pancreatic cancer. No large deletions in FANCJ were uncovered. We conclude that large FANCA deletions might contribute to breast cancer susceptibility, therefore more extensive studies on their involvement in the disease are needed. To our knowledge, this is the first report on the existence of a large-size genomic deletion in an upstream FA gene in association with familial breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5599. doi:10.1158/1538-7445.AM2011-5599