tbuckley/cabot-search huggingface dataset:
Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma
Authors: Bruna R. Correa, Patricia Rosa de Araujo, Mei Qiao, Suzanne C. Burns, Chen Chen, Richard Schlegel, Seema Agarwal, Pedro A. F. Galante, Luiz O. F. Penalva
Journal: Genome biology (2016)

Glioblastoma (GBM) is the most common and aggressive type of brain tumor. Currently, GBM has an extremely poor outcome and there is no effective treatment. In this context, genomic and transcriptomic analyses have become important tools to identify new avenues for therapies. RNA-binding proteins (RBPs) are master regulators of co- and post-transcriptional events; however, their role in GBM remains poorly understood. To further our knowledge of novel regulatory pathways that could contribute to gliomagenesis, we have conducted a systematic study of RBPs in GBM. By measuring expression levels of 1542 human RBPs in GBM samples and glioma stem cell samples, we identified 58 consistently upregulated RBPs. Survival analysis revealed that increased expression of 21 RBPs was also associated with a poor prognosis. To assess the functional impact of those RBPs, we modulated their expression in GBM cell lines and performed viability, proliferation, and apoptosis assays. Combined results revealed a prominent oncogenic candidate, SNRPB, which encodes core spliceosome machinery components. To reveal the impact of SNRPB on splicing and gene expression, we performed its knockdown in a GBM cell line followed by RNA sequencing. We found that the affected genes were involved in RNA processing, DNA repair, and chromatin remodeling. Additionally, genes and pathways already associated with gliomagenesis, as well as a set of general cancer genes, also presented with splicing and expression alterations. Our study provides new insights into how RBPs, and specifically SNRPB, regulate gene expression and directly impact GBM development.
The Spectrum Of Pathological Findings Of Kidney Injury In The Cases Of Multiple Myeloma: A Clinico-Pathological Study Of 93 Consecutive Autopsy Cases
Authors: Sohtaro Mine, Shotaro Hagiwara, Risen Hirai, Akira Tanimura, Miki Nakamura, Masataka Takeshita, Akiyoshi Miwa, Toru Igari
Journal: Blood (2013)
MAP17 Is a Necessary Activator of Renal Na+/Glucose Cotransporter SGLT2
Authors: Michael J. Coady, Abdulah El Tarazi, René Santer, Pierre Bissonnette, Louis J. Sasseville, Joaquim Calado, Yoann Lussier, Christopher Dumayne, Daniel G. Bichet, Jean-Yves Lapointe
Journal: Journal of the American Society of Nephrology (2016)

The renal proximal tubule reabsorbs 90% of the filtered glucose load through the Na+-coupled glucose transporter SGLT2, and specific inhibitors of SGLT2 are now available to patients with diabetes to increase urinary glucose excretion. Using expression cloning, we identified an accessory protein, 17 kDa membrane-associated protein (MAP17), that increased SGLT2 activity in RNA-injected Xenopus oocytes by two orders of magnitude. Significant stimulation of SGLT2 activity also occurred in opossum kidney cells cotransfected with SGLT2 and MAP17. Notably, transfection with MAP17 did not change the quantity of SGLT2 protein at the cell surface in either cell type. To confirm the physiologic relevance of the MAP17-SGLT2 interaction, we studied a cohort of 60 individuals with familial renal glucosuria. One patient without any identifiable mutation in the SGLT2 coding gene (SLC5A2) displayed homozygosity for a splicing mutation (c.176+1G>A) in the MAP17 coding gene (PDZK1IP1). In the proximal tubule and in other tissues, MAP17 is known to interact with PDZK1, a scaffolding protein linked to other transporters, including Na+/H+ exchanger 3, and to signaling pathways, such as the A-kinase anchor protein 2/protein kinase A pathway. Thus, these results provide the basis for a more thorough characterization of SGLT2 which would include the possible effects of its inhibition on colocalized renal transporters.
Brain Tumors
Authors: Charles B. Wilson
Journal: New England Journal of Medicine (1979)

PRIMARY brain tumors account for slightly less than 10 per cent of nontraumatic neurologic diseases, and for the same proportion of deaths from all neoplastic disease. Their effect is relatively greater in pediatric practice, since brain tumors constitute the most common solid tumor in this age group. The incidence of metastatic brain tumors has increased steadily, undoubtedly because the successful treatment of tumors arising outside the central nervous system has prolonged the period of risk for Central-nervous-system metastases. Nonetheless, recent advances have enhanced the diagnostic and therapeutic capabilities that determine the immediate and long-term outlook for patients with intracranial tumors. . . .
Studies of Parotid-Tumor Agent in Cultures of Leukemic Tissues of Mice
Authors: C J DAWE, L W LAW, T B DUNN
Journal: JNCI Journal of the National Cancer Institute (1959)

Attempts to detect a presumptive leukemia-inducing or leukemia-accelerating agent in leukemic tissues of the mouse in short-term cultures have been unsuccessful, since no agent was found in a variety of leukemias, including 3 from the induced passage A series of Gross. However, parotid-tumor agent (PTA) was isolated from 2 of the 3 leukemias from the Gross series. One of the positive lines subsequently converted to negative during serial transplantation. This added to the evidence that association of leukemias with PTA is largely coincidental. Continuous cultures (P388 D1) derived from a DBA/2 lymphoma initially gave no evidence of carrying PTA, but were susceptible to infection with PTA. Ten serial passages of PTA through P388 D1 were completed without evident decline of tumor-inducing activity. Thymic epithelial tumors and a variety of other tumors and lesions associated only rarely with the agent before passage through P388 D1 cultures were observed more frequently after such passage. The tumors associated with PTA form a histologically distinctive group. “Carrier” cultures of P388 D1 infected with PTA were maintained continuously for 9 months in high-serum medium. Continued presence of the agent was demonstrated by persistent tumor-inducing activity of these cultures. No completely resistant strain of mouse was found among 12 tested with PTA. Mice up to 14 days of age were found susceptible to induction of parotid tumors by PTA of high activity. Resistance of certain entire litters to PTA appeared to be correlated with maternal immunity. Tissue-culture preparations of PTA induced tumors in all litters and in nearly 100 percent of mice born of uninfected mothers.
Drug-induced dystonia in young and elderly patients
Authors: G Addonizio, G S Alexopoulos
Journal: American Journal of Psychiatry (1988)

This retrospective study examined the common notion that neuroleptic-induced dystonia is less frequent in elderly patients. The hospital records of 45 young patients and 45 elderly patients were reviewed. Thirty-one percent of the young patients developed dystonia, compared to 2% of the elderly patients. This significant difference did not appear to be the result of types of neuroleptics used, dose, or concomitant administration of anticholinergic drugs.
Doctors Afield
Authors: John Calef, Charles A. Hall
Journal: New England Journal of Medicine (1981)

DR. JOHN CALEF (1726–1812) was more often afield than at home in Ipswich practicing medicine. A list of his titles and appointments includes Surgeon, Frigate Massachusetts; Surgeon, Col. Bagley's regiment; Representative to the Provincial Assembly; twice delegate to London on behalf of the settlers of the Penobscot; Inspector of the Woods of Sagadahock, and all at Fort George; Surgeon, Inspector of the Commissary, Overseer of the Works, Chief Justice of the Peace, Superintendent of the Indians, and Chaplain. Calef was not, as his titles may suggest, a hero of the American Revolution; he was a Tory.John Calef was born . . .
Massachusetts Medical Society
Authors: John Figgis Jewett
Journal: New England Journal of Medicine (1965)

COMMITTEE ON MATERNAL WELFARECoronary Occlusion (Recanalized)With a history of at least 20 pregnancies, a thirty-five-year-old woman had only 2 living children, both delivered at term. There had been 2 term stillbirths, 1 1588-gm. (31/2-pound) full-term infant compromised by "placental insufficiency" and dead on the ninth day, 1 incomplete abortion, with dilatation and curettage, and 14 or more early abortions, all treated at home without curettage. Besides obstetric admissions, there had been several for dyspnea, chest pain, edema and cough. X-ray studies, bronchoscopy and electrocardiography had demonstrated bronchiectasis, cardiac enlargement and probable coronary-artery disease. There had been . . .
MPL W515L mutation in patients with myeloproliferative diseases
Authors: J. LI, W. XU, J. Xia, S. Zhang, L. Fan
Journal: Journal of Clinical Oncology (2008)

18019 Background: The BCR-ABL negative chronic myeloproliferative disorders (MPD) include polycythemia vera (PV), essential thrombocytosis (ET), and myelofibrosis with idopathic myelofibrosis (IMF). Although clonal hematopoiesis was observed in these disorders more than three decades ago, the molecular etiology of these disorders was not known until recently when several groups reported a somatic mutation in the JAK2 tyrosine kinase (JAK2 V617F) in most patients with PV and in a subset of patients with ET and IMF. As JAK2 V617F, MPL W515L is a novel acquired mutation that induces constitutive cytokine-independent activation of the JAK-STAT pathway in MPD. The discovery of this mutation provides a novel mechanism for activation of signal transduction in hematopoietic malignancies. Methods: To investigate its prevalence in Chinese patients with MPD, we introduced allele-specific PCR (AS- PCR) combined with sequence analysis to simultaneously screen MPL W515L and JAK2 V617F mutations in 190 MPD patients. Results: The results indicated that MPL W515L mutation was found to be harbored in only one of 102 ET patients (1.0%) and was not detected in PV, IMF, chronic myelogenous leukemia (CML) patients. Sixty eight BCR/ABL-negative MPD patients (46.3%) were found harbouring JAK2 V617F mutation (PV, 62.5% ET, 42.1% IMF 38.1%). Furthermore, MPL W515L and JAK2 V617F mutations were not detected in patients of acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), myelodysplastic syndromes (MDS) and CML. Conclusions: MPL W515L mutations may contribute to the primary molecular pathogenesis mechanism of Chinese patients with ET. No significant financial relationships to disclose.
Madonna and Child
Authors: M Therese Southgate
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Interim Guidance for Interpretation of Zika Virus Antibody Test Results
Authors: Ingrid B. Rabe, J. Erin Staples, Julie Villanueva, Kimberly B. Hummel, Jeffrey A. Johnson, Laura Rose, Susan Hills, Annemarie Wasley, Marc Fischer, Ann M. Powers
Journal: MMWR Morbidity and Mortality Weekly Report (2016)

Zika virus is a single-stranded RNA virus in the genus Flavivirus and is closely related to dengue, West Nile, Japanese encephalitis, and yellow fever viruses (1,2). Among flaviviruses, Zika and dengue virus share similar symptoms of infection, transmission cycles, and geographic distribution. Diagnostic testing for Zika virus infection can be accomplished using both molecular and serologic methods. For persons with suspected Zika virus disease, a positive real-time reverse transcription-polymerase chain reaction (rRT-PCR) result confirms Zika virus infection, but a negative rRT-PCR result does not exclude infection (3-7). In these cases, immunoglobulin (Ig) M and neutralizing antibody testing can identify additional recent Zika virus infections (6,7). However, Zika virus antibody test results can be difficult to interpret because of cross-reactivity with other flaviviruses, which can preclude identification of the specific infecting virus, especially when the person previously was infected with or vaccinated against a related flavivirus (8). This is important because the results of Zika and dengue virus testing will guide clinical management. Pregnant women with laboratory evidence of Zika virus infection should be evaluated and managed for possible adverse pregnancy outcomes and be reported to the U.S. Zika Pregnancy Registry or the Puerto Rico Zika Active Pregnancy Surveillance System for clinical follow-up (9,10). All patients with clinically suspected dengue should have proper management to reduce the risk for hemorrhage and shock (11). If serologic testing indicates recent flavivirus infection that could be caused by either Zika or dengue virus, patients should be clinically managed for both infections because they might have been infected with either virus.
SP0239 Juvenile Systemic Sclerosis
Authors: I. Foeldvari
Journal: Annals of the Rheumatic Diseases (2015)

Juvenile Systemic Sclerosis (jSSc) is an orphan diseases. The incidence is approximately 0.27 per million children. The paediatric rheumatologist pioneered in the establishment of new classification of juvenile scleroderma, which requires only one major criteria and two minor criteria. Regarding presentation of the disease in juvenile age range, they are two large cohorts published. The organ involvement pattern at the time of onset differs from the adults with Systemc Sclerosis (SSc). The mean age at onset is around 8 years. In jSSc patients at pediatric age the diffuse subset dominates and there is a very low occurrence of anticentromere antibodies. The currently running prospective juvenile scleroderma incpetion cohort project (www.juvenile-scleroderma.com), where patient data is collected with a standardized assessment protocoll prospectively, 74% of the patients have diffuse subtype too. Regarding the outcome of the juvenile patients into the adulthood, they are three publications, which show the change of pattern of the organ involvement, which could reflect a survival bias. At the adult age the subset distribution between juvenile onset or young adult onset patients does not differ anymore. The survival of the pediatric patients at 5 years is around 90 to 95%, which is significantly better than in adulthood for the same kind mostly diffuse patient population. <h3>Disclosure of Interest</h3> I. Foeldvari Consultant for: chugai, novartis, pfizer,
A Study of the Golgi Material and Mitochondria in Malignant and Benign Prostatic Tissue
Authors: Albert E. Bothe, M. D., Albert J. Dalton, Ph. D., Willard S. Hastings, M. D., Frederick O. Zillessen, M. D.
Journal: JNCI Journal of the National Cancer Institute (1950)

Journal Article A Study of the Golgi Material and Mitochondria in Malignant and Benign Prostatic Tissue Get access Albert E. Bothe, M. D., Albert E. Bothe, M. D. Search for other works by this author on: Oxford Academic PubMed Google Scholar Albert J. Dalton, Ph. D., Albert J. Dalton, Ph. D. Search for other works by this author on: Oxford Academic PubMed Google Scholar Willard S. Hastings, M. D., Willard S. Hastings, M. D. Search for other works by this author on: Oxford Academic PubMed Google Scholar Frederick O. Zillessen, M. D. Frederick O. Zillessen, M. D. Jeanes Hospital, Philadelphia, Pa., and National Cancer Institute, National Institutes of Health, U. S. Public Health Service, Bethesda, Md. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 11, Issue 2, October 1950, Pages 239–251, https://doi.org/10.1093/jnci/11.2.239 Published: 01 October 1950 Article history Received: 28 March 1950 Published: 01 October 1950
SP0122 How to Evaluate Subclinical Atherosclerosis with us of the Carotid Artery + Demo
Authors: D.L.C. Joelle
Journal: Annals of the Rheumatic Diseases (2015)

Common carotid artery intima-media thickness (CIMT) has been shown to predict cardiovascular risk. Measuring the far wall may be difficult. the common carotid artery is the least variable method for assessing IMT, however obtaining adequates images of all carotid artery segments may be difficult. The reproductibilty of CIMT measurements is improved by the development of automated edge-tracking software. The manual edge tracking needs several measurements and a mean of the measurements. Evaluate IMT progression suppose to use the same methods of measurements, automated edge-tracking software or manual measurement. In short series, both measurements were released on a same patient, and same ultrasound scan, to evaluate the difference of the results <h3>Disclosure of Interest</h3> None declared
A Leu117 → Trp Mutation Within the RGD-Peptide Cross-Linking Region of β3 Results in Glanzmann Thrombasthenia by Preventing αIIbβ3 Export to the Platelet Surface
Authors: Ramesh B. Basani, Deborah L. Brown, Gaston Vilaire, Joel S. Bennett, Mortimer Poncz
Journal: Blood (1997)
Is the basic reproductive number (R0) for measles viruses observed in recent outbreaks lower than in the pre-vaccination era?
Authors: P Plans Rubió
Journal: Eurosurveillance (2012)

Preview this article: Is the basic reproductive number (R0) for measles viruses observed in recent outbreaks lower than in the pre-vaccination era?, Page 1 of 1 < Previous page | Next page > /docserver/preview/fulltext/eurosurveillance/17/31/art20233-en-1.gif
SAT0120 Ninja, A Japanese Rheumatoid Arthritis Database, Demonstrated that the Size and Number of Swollen Joints Correlated with Increased Systemic Inflammation Markers. Digital Joint Swelling Showed Only Trace Increases of Serum C-Reactive Protein and Erythrocyte Sedimentation Rate
Authors: K. Shimada, A. Komiya, K. Onishi, T. Kise, Y. Miyoshi, Y. Nagai, E. Kikuchi, T. Nunokawa, T. Sawaki, N. Yokogawa, S. Sugii, S. Tohma
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> Polyarthritis caused by rheumatoid arthritis has wide variety in size and number of affected joints. In diagnosis and evaluation, swollen joint count and systemic inflammation markers (<i>e.g.</i> serum C-reacive protein (CRP) and erythrocyte sedimentation rate (ESR)) are considered independently. However, when systemic inflammation markers are supposed to reflect the total inflammation in the whole body, the level of the markers should vary according to the size and the number of the swollen, that is, inflamed joints. <h3>Objectives</h3> To investigate the correlation between the size of swollen joints with systemic inflammation markers using a large-scale patient database. <h3>Methods</h3> <i>NinJa</i> is the largest database of rheumatoid arthritis in Japan in which more than 10,000 patients are registered. Data registered to the <i>NinJa</i> database in 2012 were used. Sixty-six joints were classified into 3 size categories, namely, the digital joints (distal interphalangeal joints, proximal interphalangeal joints, interphalangeal joints, metacarpophalangeal joints and metatarsophalangeal joints) and acromioclavicular joints as small joints (joint surface area<sup>1</sup> &lt;10 cm<sup>2</sup>), the knees as large joints (joint surface area<sup>1</sup> &gt;100 cm<sup>2</sup>), and the others as medium-sized joints (joint surface area<sup>1</sup> between 10 cm<sup>2</sup> and 100 cm<sup>2</sup>). Patients with swollen joints limited to one joint-size category were grouped by swollen joint count and compared with patients with no swollen joint. 8574 cases were analyzed. Patients receiving anti-interleukin-6 or Janus-kinase-inhibiting therapies were excluded. <h3>Results</h3> Patients with larger numbers of swollen joints tended to show higher levels of both serum CRP and ESR in each joint-size category (Figure 1). In the medium-sized, or knee joint group, these levels were significantly higher than in the no-swelling group and small joint group with the same swollen joint count. Linear regression analysis revealed increments of CRP (mg/L) (ESR (mm/1hr) [95% confidence interval]) per swollen joint as 4.6 [3.7-5.5] (8.9 [6.5-11]) in the knee, 2.3 [2.0-2.7] (4.9 [4.0-5.9]) in medium-sized joints, and 0.56 [0.35-0.77] (0.89 [0.39-1.38]) in the small joints. <h3>Conclusions</h3> The strength of swelling in the joints affecting systemic inflammation markers increased with the involvement of larger and/or more numerous joints. <h3>References</h3> Am J Med Sci. 1956;232:150-5 <h3>Disclosure of Interest</h3> None declared
REM sleep and adaptation of psychiatric patients: an application of sleep studies
Authors: R Greenberg, C A Pearlman
Journal: American Journal of Psychiatry (1976)

The authors used sleep laboratory data to predict responses to psychiatric treatment. The predictions were based on the assumptions that REM latency reflects the need for dreaming and therefore the need for adaptation and that REM time reflects the capacity for the adaptive work associated with REM sleep. Dream recall and dream content were considered indicators of psychological mindedness and the patient's current conflicts. With this material, statistically significant predictions of such outcomes as good response to psychotherapy, elopement, or need for medication were made. The results of this study support the hypothesis that REM sleep is involved in adaptation.