tbuckley/cabot-search huggingface dataset:
Abstract 4300: Constitutive activation of Stat3 increases hair follicle progenitor population at the expense of bulge region keratinocyte stem cells
Authors: Dharanija Rao, Steve Carbajal, Kaoru Kiguchi, John DiGiovanni
Journal: Cancer Research (2010)

Abstract Mouse skin epidermis is maintained throughout life by a highly proliferative compartment located in the outer root sheath called the hair follicle bulge. Stem cells located in the mouse hair follicle bulge region have self-renewing, slow cycling and quiescent characteristics and maintain hair and epidermal growth. In the present study, we examined the impact of Stat3 activation on the number and proliferative capacity of bulge region keratinocyte stem cells (KSCs). K5. Stat3C transgenic mice express a constitutively active/dimerized form of Stat3 called Stat3C via the bovine keratin 5 (K5) promoter. The K5 promoter targets expression of Stat3C in the basal layer of the epidermis as well as in the bulge region KSCs. We analyzed the number of KSCs using the bulge specific stem cell markers CD34 and α6 integrin. The number of CD34+ve/α6 +ve cells was significantly reduced in Stat3C mice as compared to the non-transgenic littermates. Sca-1 positive cells reside in the infundibulum region of the hair follicle. We analyzed the Sca-1 expressing population in the total hair follicle cells and found that the number of Sca-1 expressing cells was increased in the Stat3C mice. Activation of Stat3, in bulge region KSCs appeared to cause epidermal stem cells to exit the stem cell compartment. To understand how Stat3 exerts these effects we studied the expression of Stat3 target genes like Myc and cyclin D1. Both Myc and cyclin D1 were found to be upregulated in total hair follicle cells in the transgenic mice. In addition, the levels of both α6 and β1 integrin were reduced in total hair follicle cells of the Stat3C mice supporting the hypothesis that activation of Stat3 causes stem cell to exit from the bulge region. Overall, these studies suggest that Stat3 is critical for the behavior, growth properties and quiescent characteristics of bulge region KSCs. Supported by NIH Grant CA76520. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4300.
SP0061 Fibromyalgia – What Really Works? from Ancient to Recent Managements
Authors: W. Häuser
Journal: Annals of the Rheumatic Diseases (2014)

<h3></h3> Multiple systematic reviews of randomised controlled trials (RCTs) demonstrated that no therapy (complementary/alternative, drug, physical, psychological) in FM "really works" in the sense of substantial symptom relief in the majority of patients. On average, the effects of all these treatments on FM-symptoms are small and from a clinical point of view not meaningful. Network meta-analyses demonstrated that "old drugs" such as amitryptiline or fluoxetine did not differ from "new" drugs such as duloxetine, milnacipran and pregabalin in terms of symptom reduction and tolerability. In the short run, the effects of these drugs were comparable to the ones of aerobic exercise and cognitive behavioral therapies. However, there is only evidence of sustained (but declining) positive effects of aerobic exercise and cognitive behavioral therapies after the end of treatment. Clinicians should not treat average values, but treatment responders. Detailed analysis of RCTs of pharmacological and non-pharmacological studies demonstrated that there is a minority of patients with substantial symptom relief with no or tolerable side effects (sustained responders). Until now, there are no predictors of sustained responders available. It is the art of medicine to identify treatment responders. Recent guidelines (Canada, Germany, Israel) emphasized graduated treatment according to the severity of FM. Patients with mild FM do not need a specific treatment. In severe cases, drug therapy should cover not only pain and be tailored to the symptoms of the patient. Duloxetine might be preferred in patients with co-morbid major depression and amitriptyline or pregabalin in case of severe sleeping problems. A recent study which combined aerobic exercise with tailored psychological therapies showed promising long-term results. Finally treatment recommendation of FM should balance potential benefits, harms (tolerability, safety) and availability of treatment options. Traditional RCTs may not be the method of choice to answer all these questions. Alternative approaches should be developed and evaluated, e.g. systematic comparative effectiveness studies of health care registry data, use of patient registries and consumer reports. <h3>Conflicts of interest</h3> I received a consulting honorarium by Daiichi Sankyo and honororia for educational lectures by Abbott, MSD Sharp&amp;Dhome and Pfizer within the previous three years. I am member of medical associations of internal medicine, psychosomatic medicine, psychotherapy and sports medicine. I am the head of the steering committee of the German guideline on the management of FMS. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.6185
380 RAPAMYCIN MODULATES THE COURSE OF HEPATITIS B VIRUS (HBV) INFECTION IN HYDRODYNAMIC INJECTION MOUSE MODEL: THE ROLE OF Treg IN HBV PERSISTENCE
Authors: S. Huang, Z. Song, B. Wang, J. Wang, Z. Zhu, B. Zhu, X. Feng, M. Lu, D. Yang
Journal: Journal of Hepatology (2013)
SAT0522 Value of the Doppler-Ultrasonography for the Diagnosis of Temporal Arteritis
Authors: S. García Carazo, E. De Miguel, C. Castillo, P. Alcocer Amores, E. Martín Mola
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Giant cell arteritis (GCA) or Temporal Arteritis is the most common systemic vasculitis in adults. Usually, the diagnosis is based on the American College of Rheumatology (ACR) classification criteria published in 1990, and on the temporal artery biopsy. But according to the literature, the biopsy can be negative in up to 68% of patients with GCA. Today, the validity of Doppler Ultrasound has been shown in at least three metaanalysis. <h3>Objectives</h3> Assess the value of Doppler Ultrasonography for the diagnosis of GCA, compared with ACR criteria and biopsy, using the definitive clinical diagnosis of the patient as gold standard. <h3>Methods</h3> Two hundred seventy-six patients with clinical suspicion of GCA were evaluated. All of them underwent Doppler Ultrasonography of temporal arteries. Only 92 patients underwent biopsy, and the gold standard was the final diagnosis at least three months after the clinical trial and initiation of treatment. The topographic involvement (number of branches affected by ultrasound) of 267 patients was recorded. <h3>Results</h3> Of the 276 patients studied, 189 were women and 87 men (68.5% vs 31.5%), with a mean age of 75.81 ± 9.02. Forty-eight percent had definite diagnosis of GCA while 55.79% met the ACR criteria and 45.28% had ultrasound signs compatible with GCA. Of the 92 biopsies, 24 (26%) were positive. The validity (sensitivity and specificity) and Security (positive predictive value [PPV], negative predictive value [NPV], Likelihood Ratio [LR] + and LR -) of diagnostic tests used were as follows: According to topographic involvement, 17 patients had a single branch affected with 23.5% false positives. There were only 2% of false positives in the 100 patients with involvement of two or more branches. The only false positive in four branches affected group was a skull base osteomyelitis. ESR showed a variation between patients with ACG and those with othe rdiagnoses not significant (64.05 ± 58.15 vs 29.8 ± 32.3, p = 0.12), while the PCR variation was significant between patients with and without GCA (59.51 ± 63.4 vs. 31.6 ± 53.7, p = 0.016). <h3>Conclusions</h3> Doppler Ultrasonography shows great validity and safety as a diagnostic test of GCA, and its use in clinical practice and in the diagnostic criteria of the disease should be valued. <h3>Disclosure of Interest</h3> None Declared
Refluxtions on Esophageal Cancer: Can We Swallow the Changes?
Authors: Joel Tepper
Journal: Journal of Clinical Oncology (2000)

Article Tools EDITORIAL Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2000.18.3.453 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 10653859 Refluxtions on Esophageal Cancer: Can We Swallow the Changes? Joel TepperxJoel TepperSearch for articles by this author Show More University of North Carolina, Chapel Hill, NC https://doi.org/10.1200/JCO.2000.18.3.453 Full Text PDF Figures and Tables "Refluxtions on Esophageal Cancer: Can We Swallow the Changes? ." Journal of Clinical Oncology, 18(3), p. 453© 2000 by American Society of Clinical Oncology
Double- vs. single-balloon enteroscopy: and the winner is…
Authors: M. Manno, C. Barbera, H. Bertani, R. Manta, V. Mirante, R. Conigliaro
Journal: Endoscopy (2012)

We read with interest the review by May on balloon-assisted enteroscopy [1]. The author concluded that double-balloon enteroscopy (DBE) is superior to single-balloon enteroscopy (SBE). However, we would like to make some comments.
Combined modality therapy versus chemotherapy alone as an induction regimen for primary central nervous system lymphoma: a cost-effectiveness analysis
Authors: A. Prica, K. Chan, M. Cheung
Journal: Neuro-Oncology (2014)

In immunocompetent patients with primary central nervous system lymphoma (PCNSL), combined modality therapy (CMT) using high-dose methotrexate and radiotherapy (WBRT) has improved response rates compared with chemotherapy alone. The trade-off is delayed and potentially devastating treatment-related neurotoxicity (NT). A cost-effectiveness analysis using a Markov model compared CMT with chemotherapy alone in age-stratified patients with PCNSL. Baseline probabilities were derived from a systematic literature review. Direct and lost productivity costs were collected from a Canadian perspective and presented in Can$ in 2011. Outcomes were life expectancy, quality-adjusted life years (QALYs), and incremental cost-effectiveness ratio. The quality-adjusted life expectancy was 1.55 QALYs for CMT and 1.53 QALYs for chemotherapy alone. In younger patients (aged <60 years), CMT yielded 2.44 QALYs, compared with 1.89 QALYs for chemotherapy alone, yielding an expected benefit with CMT of 0.55 QALYs or 6.6 quality-adjusted months. The CMT strategy dominated in younger patients, as it was Can$11 951 less expensive than chemotherapy alone. The chemotherapy-alone strategy dominated in older patients, as it was Can$11 244 less expensive than CMT, and there was no difference in QALYs between the strategies. The model was robust in sensitivity analyses of key variables tested through the plausible ranges obtained from costing sources and published literature. The preferred induction strategy for younger patients with PCNSL appears to be CMT, which minimized cost while maximizing life expectancy and QALYs. This analysis confirms that the preferred strategy for older patients is chemotherapy alone.
Abstract 753: Reciprocal regulation of HOXB13 and androgen receptor in prostate cancer.
Authors: Shuangling Chen, Dorhyun Johng, William B. Isaacs
Journal: Cancer Research (2013)

Abstract The androgen receptor (AR), a member of the nuclear receptor superfamily of ligand-regulated transcription factors, plays a pivotal role in the initiation and progression of prostate cancer. HOXB13, a prostate-specific homeodomain-containing transcription factor, interacts with AR to regulate the cellular response to androgens (Norris et al. Mol Cell 2009). Our group recently identified a rare but recurrent germline mutation (G84E) in HOXB13 as a prostate cancer susceptibility allele by sequencing the 17q21-22 region of the genomic DNA from families with hereditary prostate cancer. (Ewing et al. NEJM 2012). Our research goal is to study how HOXB13 regulates AR function and how this mechanism might be affected by mutant HOXB13. The overexpression of wild type HOXB13 in LNCaP cells led to an increased expression of AR at the mRNA and protein levels; this induction of AR was attenuated with HOXB13 G84E over-expression. Upon the reduction of endogenous HOXB13 by siRNA in LNCaP cells, the amount of AR decreased at the mRNA and protein levels, indicating that HOXB13 promotes the expression of AR. Conversely, when AR was knocked down with siRNA, the level of HOXB13 transcripts and proteins increased, indicating that AR suppresses HOXB13 expression. Furthermore, co-immunoprecipitation experiments demonstrate that FOXA1, the pioneer factor implicated to control prostate-specific transcription of HOXB13 (McMullin et al. 2009), interacts with AR and HOXB13. These analyses implicate at least a three factor mechanism whereby the levels of critical components of prostate-specific androgen signaling network might recruit and/or titrate one another, and suggest this network may be perturbed by mutations in HOXB13. Since LNCaP cells contain a mutated copy of HOXB13 (L144P), these analyses need to be performed in additional prostate cancer cells before general conclusions can be reached. Citation Format: Shuangling Chen, Dorhyun Johng, William B. Isaacs. Reciprocal regulation of HOXB13 and androgen receptor in prostate cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 753. doi:10.1158/1538-7445.AM2013-753
SP0104 What Does Evidence Tell us About Gender Differences in Psychological Impact and Ways of Coping in People with RMD?
Authors: M. B. Kool
Journal: Annals of the Rheumatic Diseases (2013)

<h3></h3> It can be a great challenge for men and women to learn to deal with a rheumatic disease and its consequences. There are numerous indications in scientific literature that men and women react differently to stress. It has, for instance, been proposed that female reactions are characterized by a "tend-and-befriend" pattern, while male behavior is characterized by a "fight-or-flight" pattern. "Tend and befriend" refers to behavior that involves protection of offspring (tending) and seeking out the social group for mutual defense (befriending). A fight-or-flight pattern is characterized by confronting the stressor (fight) or flee (flight), either literally or through avoidant coping, such as social withdrawal. There are also other examples from daily life suggesting that women and men indeed react differently. For example, women show more emotions and like to talk with other women about their problems, while men perhaps process emotions by seeking distraction such as watching soccer with other men. Social roles are also different for men and women. Women more often take care of the children and do a larger part of the housekeeping. These differences in roles and coping between men and women may affect the psychological impact of a rheumatic disease. This would suggest a gender-sensitive approach in research and health care. In this presentation, I will review research that used a gender-sensitive approach and present the results with regard to gender differences in psychological impact and ways of coping in people with rheumatic diseases. <h3>Disclosure of Interest</h3> None Declared
Detection of Sarcomatoid Lung Metastasis With 68GA-PSMA PET/CT in a Patient With Prostate Cancer
Authors: Llanos Geraldo, Francesco Ceci, Christian Uprimny, Dorota Kendler, Irene Virgolini
Journal: Clinical Nuclear Medicine (2016)

A 70-year-old man with prostate cancer (adenocarcinoma; pT3aN0Mx; GS: 4 + 4) underwent radical prostatectomy and lymph node dissection in February 2008. In December 2009, biochemical recurrence occurred and prostate-specific antigen progressively increased to 4.63 ng/mL despite local salvage radiotherapy and androgen deprivation. 68Ga-PSMA PET/CT showed a positive left iliac lymph node and a pathological left pulmonary lesion, which was highly positive in a subsequent 18F-FDG PET/CT. Lymph node resection confirmed an adenocarcinoma metastasis of the prostate cancer and lung surgery demonstrated a sarcomatoid metastasis of prostate cancer. After surgery, prostate-specific antigen decreased to 0.03 ng/mL.
A5.29 Spontaneous Production of Anti-Citrullinated Protein Antibodies in Cultures of Peripheral Blood Mononuclear Cells and Synovial Fluid Mononuclear Cells Isolated from Patients with Rheumatoid Arthritis
Authors: Priscilla F Kerkman, Yoann Rombouts, Ellen IH van der Voort, Leendert A Trouw, Tom WJ Huizinga, René EM Toes, Hans U Scherer
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background and Objectives</h3> Anti-citrullinated protein antibodies (ACPA) are among the most important molecular candidates that could drive the inflammatory immune response in a subset of patients with rheumatoid arthritis (RA). So far, however, little is known on the phenotype and functional characteristics of ACPA producing B cells. Therefore, we studied ACPA producing B cells using ex-vivo cultures of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC). <h3>Materials and Methods</h3> PBMC as well as SFMC from patients with ACPA-positive RA were cultured in 96 well plates without the addition of exogenous stimuli. Cultures were maintained for several weeks, with weekly complete replacement of the culture medium. Every week, supernatants were assessed for the presence of ACPA-IgG and total IgG by ELISA. B cell subsets within the culture populations were determined by flow cytometry at several time points. <h3>Results</h3> Circulating, spontaneously ACPA producing B cells were readily detectable in peripheral blood of ACPA positive RA patients, but not in ACPA negative RA patients or healthy donors. FACS sorting experiments comparing isolated B cell subsets located spontaneous ACPA production to the plasmablast compartment. Memory B cells were capable of ACPA production upon stimulation. In some culture wells, ACPA production was stable and detectable for up to 3 months. In a similar manner, we observed spontaneous, long-lasting ACPA production in (paired) SFMC cultures. The latter showed an up to 200 fold increase in ex vivo ACPA production compared to PBMC, but only a minor increase in the secretion of non-specific IgG. B cell numbers in PBMC and SFMC were comparable in the starting population. <h3>Conclusions</h3> ACPA specific plasmablasts circulate in the peripheral blood of patients with ACPA positive RA. Upon isolation, peripheral blood B cells can secrete ACPA spontaneously for several months. This observation suggests that ACPA specific B cells are either continuously recruited from the memory compartment, or that a subset of ACPA specific plasmacells might have the capacity to survive for extended periods of time. In SFMC, the frequency of ACPA specific B cells is strongly increased. These observations point to a continuously active, ACPA specific immune response in RA.
Values of endoscopic ultrasonography on diagnosis and treatment of duodenal stromal tumor
Authors: Hu Feng-ling, Xu Guo-Qiang, Teng Xiao-Dong, Chen Hong-Tan, Shan Guo-Dong
Journal: Endoscopy (2011)

Objective: To explore the clinical values of endoscopic ultrasonography (EUS) on diagnosis and treatment of duodenal stromal tumor.
1417 THE MACROPHAGE-DERIVED SERUM BIOMARKER SOLUBLE CD163 INDEPENDENTLY PREDICTS LIVER CIRRHOSIS IN THE GENERAL POPULATION
Authors: H.J. Møller, R. Frikke-Schmidt, S.K. Moestrup, B.G. Nordestgaard, A. Tybjærg-Hansen
Journal: Journal of Hepatology (2012)
WNT11 PRETREATMENT PROTECTS AGAINST OXIDATIVE STRESS-INDUCED APOPTOSIS BY STAT3 ACTIVATION
Authors: Anweshan Samanta, Lei Chen, Lin Zhao, Arash Davani, Kashyap Choksi, Guangming Cheng, Amy Cantilena, Robert Vincent, Magdy Girgis, Buddhadeb Dawn
Journal: Journal of the American College of Cardiology (2015)
Abstract A17: miRNA silencing in malignant melanoma: Mechanisms, regulation, and potential implications
Authors: Raya Leibowitz-Amit, Liron Zehavi, Dror Avni, Yehezkel Sidi
Journal: Clinical Cancer Research (2010)

Abstract Metastatic melanoma is a devastating disease with limited treatment options. miRNAs are small RNA species within cells whose aberrant expression has been implicated in cancerous transformation, in some cases by a mechanism of cellular de-differentiation. The role of miRNA in melanoma is still not known. We recently observed that the expression of miRNAs from a large intergenic miRNA cluster is significantly down-regulated or absent in melanoma cell lines in comparison to normal melanocytes. This cluster resides within a parentally imprinted chromosomal region implicated in development and differentiation, and the miRNAs in this region are normally expressed selectively only in brain and skin. This chromosomal region was shown to be involved in the pathogenesis of ovarian carcinoma, and several of the miRNAs within it were shown to have tumor suppressor functions in other cellular models. Our results demonstrate that in some melanoma cell lines, there is a chromosomal deletion or loss of heterozygosity in the cis-acting differentially-methylated regulatory region of this cluster. Conversely, in other cell lines we were able to re-express some of these miRNAs using epigenetic modifiers (de-methylating agents and histone de-acetylase inhibitors), suggesting that epigenetic aberrations take part in their silencing. We are now assessing whether miRNA re-expression or ectopic expression decreases the tumorigenic and metastatic potential of melanoma cells. Our results suggest that aberrant regulation of this chromosomal region has a role in the pathogenesis of melanoma, and may even lead, in the future, to the development of new therapeutic strategies and bio-markers in this yet incurable disease. Citation Information: Clin Cancer Res 2010;16(7 Suppl):A17
Electromagnetic Bougienage to Lengthen Esophageal Segments in Congenital Esophageal Atresia
Authors: W. Hardy Hendren, J. Richard Hale
Journal: New England Journal of Medicine (1975)

Some cases of esophageal atresia, either with or without tracheoesophageal fistula, are not suited for primary anastomosis. To avert the need for colon interposition in two such infants, intermittent electromagnetic force was used to pull together "bullets" placed in the esophageal ends. This method elongated and enlarged the esophageal segments enough to accomplish their anastomosis later. This approach appears feasible to use for infants whose esophageal malformation does not permit primary repair. It may also be applicable to cases of imperforate anus with a hight pouch.
FLUOROSCOPIA VS NO FLUROSCOPIA EN EL DRENAJE TRANSMURAL GUIADO POR USE DE COLECCIONES ABDOMINALES
Authors: CF Consiglieri, JB Gornals, S Salord, M De La Hera, LT Sanchez Salido, E Garcia-Recio, J Busquets, N Pelaez, R Sanzol, J Fabregat
Journal: Endoscopy (2014)

Introducción: Habitualmente se ha utilizado fluoroscopia durante la práctica de drenajes transmurales guiados por USE, aunque con la mejoria y conocimiento de la técnica junto a la innovación de los utensilios, recientemente se han descrito experiencias sin la ayuda de fluoroscopia.
Irritable Bowel Syndrome
Authors: Richard B. Lynn, Lawrence S. Friedman
Journal: New England Journal of Medicine (1993)

Irritable bowel syndrome is one of a group of disorders that includes chest pain of unexplained origin, nonulcer dyspepsia, and biliary dyskinesia. These chronic disorders are frequently considered functional, because no specific structural or biochemical cause has been found1. In all likelihood, the pathogenesis involves disordered motility or altered visceral sensation, although no consistent abnormality has been identified. In addition, psychosocial factors affect the clinical expression of these disorders.Definition and Clinical PresentationPatients with irritable bowel syndrome have a broad range of symptoms well known to most clinicians. The symptoms may be continuous or intermittent but should be . . .