tbuckley/cabot-search huggingface dataset:
Abstract 438: Development of an autoantibody panel that reflects disease pathogenesis in ovarian cancer
Authors: Makoto Kobayashi, Clayton R. Boldt, Wei-Lei Yang, Clemente Aguilar-Bonavides, Hong Wang, Zhen Lu, Robert C. Bast, Samir M. Hanash
Journal: Cancer Research (2016)

Abstract Introduction The serous subtype of ovarian cancer is responsible for most ovarian cancer deaths. When patients are diagnosed with early stage disease localized to the ovary or pelvis, current therapies can achieve survival rates up to 75-90%. However, approximately 70% of cases are diagnosed at an advanced stage, with a 5-year survival rate of less than 20%. A strategy based on autoantibodies which occur early during tumor development has merit for early cancer detection. We have applied a combined approach of mass spectrometry of circulating antigen-antibody complexes and with high-density protein microarrays to develop ovarian cancer serum markers based on autoantibody reactivity. Materials and methods Serum samples were obtained from women diagnosed with ovarian cancer (cases) and healthy controls undergoing ovarian cancer screening, women undergoing gynecologic surgery for various conditions but with normal ovarian pathology (surgical controls), and women without malignancy but with benign ovarian disease (benign controls). All samples were obtained following Institutional Review Board approval and informed consent. For autoantibody analysis, we used recombinant protein arrays containing 5,005 recombinants arrayed in duplicate and Alexa 647-labeled anti-human IgG were purchased from Invitrogen. Circulating Immunoglobulin (Ig) bound antigen analysis was performed using LC-MS/MS. Results Autoantibodies against 20 targets were identified in ovarian cancer patients compared to healthy, surgical and benign controls by protein array analysis with P&amp;lt;0.01. Ingenuity Pathway Analysis of these 20 antigens, exhibited major nodes of p53, MYC, and ESR1 associated networks reflecting ovarian cancer pathogenesis. Concordant findings were observed with LC-MS/MS analysis of Ig-antigen complexes. Conclusion We have uncovered a set of 20 protein antigens associated with the development of autoantibodies in ovarian cancer that reflect disease pathogenesis and that have potential relevance for early detection. Citation Format: Makoto Kobayashi, Clayton R. Boldt, Wei-Lei Yang, Clemente Aguilar-Bonavides, Hong Wang, Zhen Lu, Robert C. Bast, Samir M. Hanash. Development of an autoantibody panel that reflects disease pathogenesis in ovarian cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 438.
37P Serum microRNAs as potential biomarker for screening colorectal cancer patients
Authors: L. Ng, T. Wan, A. Chow, J. Man, D. Iyer, W.-K. Leung, T. Yau, O. Lo, D. Foo, J. Poon, W.-L. Law, R. Pang
Journal: Annals of Oncology (2015)
Abstract 843: Spontaneous immortalization of human mammary epithelial cells from a woman with a germline STK11 mutation
Authors: David Euhus, Dawei Bu, Michael Considine, Leslie Cope
Journal: Cancer Research (2016)

Abstract Spontaneous immortalization of benign mammary epithelial cells (MEC) in culture is very rare. Models of MEC immortalization suggest that cells escape M0 growth arrest (selection) by silencing p16 expression after which they encounter a second growth arrest, M1 (agonescence), characterized by telomere shortening and profound genomic instability. Pre-selection, post-selection, and spontaneously immortalized MEC from a woman with a germline STK11 mutation were subjected to a comprehensive “OMICS” evaluation including miRNA, transcriptome, methylome, exome, and comparative genomic hybridization (CGH) in order to understand the molecular events associated with MEC immortalization. Principle component analysis of gene expression identified 4 significant components, the first of which showed the greatest separation between the different time points. The most notable expression changes were upregulation of FOXQ1, IL1-related genes, Ras-related genes, and fibronectin and downregulation of COL1, COL6, CK19, and CDH1. MEC gradually acquired global hypomethylation of non-CpG DNA and regional hypermethylation of CpG islands (including p16). A classifier based on methylation pattern showed that immortalized cells moved away from identity with The Cancer Genome Atlas (TCGA) benign samples towards TCGA breast cancer samples. Immortalization was associated with a modest accumulation of mutations and a modestly greater number of DNA copy number alterations than was observed in earlier passage cells but no evidence of general genomic instability. Immortal cells showed a large deletion at 10q13-15 and deletions at 10p11-15 and 17p11-13 that were associated with reduced expression of genes in those regions. Post-selection bulk cell populations are quite distinct from early passage cells, but are not direct precursors of the immortal cells as some DNA copy number alterations acquired by post-selection cells were not observed in the immortalized cells. Rather, in this instance, it is likely that post-selection cells established conditions conducive to the immortalization of a rare cell in the mix. This has implications for the development of tissue-based approaches for breast cancer risk stratification. Citation Format: David Euhus, Dawei Bu, Michael Considine, Leslie Cope. Spontaneous immortalization of human mammary epithelial cells from a woman with a germline STK11 mutation. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 843.
Robotic surgery for prostate cancer achieves similar outcomes to open surgery, study shows
Authors: Susan Mayor
Journal: BMJ (2016)

Robotic prostatectomy has achieved similar outcomes to open surgery in removing cancerous tissue and preserving urinary and sexual function in men with localised prostate cancer, early results from a study to compare these two approaches have shown.1

The study recruited 326 men (aged 35 to 70) with newly diagnosed localised prostate cancer who had chosen to be treated with surgery. They were randomly assigned to robot assisted laparoscopic prostatectomy or open radical retropubic prostatectomy, led by two surgeons at the Royal Brisbane and Women’s Hospital, Brisbane, Australia. Use of robotic surgery for prostate cancer has increased rapidly since its introduction in 2000, but trial data comparing the two surgical approaches have been limited.

Results from …
LP42 : Safety and efficacy of novel antivirals in kidney transplant recipients with chronic hepatitis c virus (HCV) infection
Authors: M.V. Lin, M.E. Sise, M. Pavlakis, J.M. Hanifi, A.E. Rutherford, N. Elias, E.C. Heher, M.P. Curry, L.V. Riella
Journal: Journal of Hepatology (2015)
Relaxin serum levels in acute heart failure are associated with pulmonary hypertension and right heart overload
Authors: Mariana Pintalhao, Paulo Castro‐Chaves, Francisco Vasques‐Novoa, Francisco Gonçalves, Luís Mendonça, Ricardo Fontes‐Carvalho, Patrícia Lourenço, Pedro Almeida, Adelino Leite‐Moreira, Paulo Bettencourt
Journal: European Journal of Heart Failure (2016)
Abstract 1929: The novel role of microRNA-128 in proneural to mesenchymal subtype transition in glioblastoma stem cells by targeting components of pro-oncogenic Polycomb Repressor Complex
Authors: Arun K. Rooj, Marco Mineo, Franz Ricklefs, Agnieszka Bronisz, Ennio Chiocca, Jakub Godlewski
Journal: Cancer Research (2016)

Abstract Heterogeneous glioblastoma multiforme (GBM) was categorized based on transcriptional signatures into four subtypes (proneural (PN), neural, classical, and mesenchymal (MES)). In order to develop effective targeted therapeutic strategies, understanding the heterogeneous gene expression and molecular features of these subtypes is crucial. De-regulation of microRNA expression and activity has been shown to play an important role in tumor initiation and progression, including gliomagenesis. We have previously reported that expression of microRNA-128 (miR-128) is significantly down regulated in GBM and it diminishes self-renewal of GBM stem-like cells (GSCs) and sensitizes them to irradiation. Proneural-to-mesenchymal transition (PMT) manifested by concomitant up regulation of MES markers and down regulation of PN markers, is associated with increased malignancy, therapy-resistance and worse prognosis, but the underlying causes of PMT have not been convincingly characterized yet. In this study, we have demonstrated that miR-128 can regulate the PMT in GSC subsets. We showed that the expression of miR-128 in PN GSCs was significantly higher compared to MES subtype. As a tumor suppressive microRNA, miR-128 inhibited the MES GSC-specific high expression of Bmi1 and Suz12, two components of Polycomb Repressor Complexes (PRC) 1 and 2, respectively. In both GSC subtypes, miR-128 driven targeting of PRCs suppressed their epigenetic activity measured by ubiquitination of H2AK119 and tri-methylation of H3K27. Stable down regulation of miR-128 in PN GSCs significantly increased the expression of MES-specific gene signature (BCL2A1, CD44, WT-1, LYN, and MET) while its stable up regulation in MES GSCs resulted in the restoration of PN specific gene signature (CD133, SOX2, NES, OLIG2, and NOTCH1). We also showed that stable expression of miR-128 in GSCs could regulate the process of irradiation-induced PMT. Our in vivo studies showed the anti-tumorigenic role of miR-128 in both PN and MES GSC-derived intracranial tumor models. Taken together, we demonstrated that altering levels of miR-128 was sufficient to cause or reverse PMT, most likely by targeting the level/functions of PRCs and their target genes in GBM. Citation Format: Arun K. Rooj, Marco Mineo, Franz Ricklefs, Agnieszka Bronisz, Ennio Chiocca, Jakub Godlewski. The novel role of microRNA-128 in proneural to mesenchymal subtype transition in glioblastoma stem cells by targeting components of pro-oncogenic Polycomb Repressor Complex. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1929.
Myokine: a novel target for exercise to improve cognitive function?
Authors: Zhe-Cheng Jiang, Ri-Yue Jiang, Huan Li, Bin Zhu
Journal: British Journal of Sports Medicine (2016)

Recently, we read with great interest the paper by Zheng et al ,1 in which the authors who performed an excellent meta-analysis concluded that aerobic exercise had a physiological benefit to improve cognitive function in patients with mild cognitive impairment. We appreciate this finding and would like to present a hypothesis that myokine triggered by physical aerobic exercises might also play a pivotal role in the improvement of cognitive impairment.

Physical exercise is of great importance to the health of brain. Increasing evidence shows that regular exercise could increase the activity of skeletal muscle and, as a …
C-reactive protein at 4 days after surgery as an early predictor of serious infectious complications following esophageal cancer surgery.
Authors: Kazuki Kano, Takashi Ogata, Yukio Maezawa, Kenki Segami, Tetsushi Nakajima, Kousuke Ikeda, Tsutomu Sato, Haruhiko Cho, Takaki Yoshikawa
Journal: Journal of Clinical Oncology (2016)

10 Background: Serious infectious complications (SICs), frequently observed morbidities, have been reported to be related not only with short-term mortality but also with poor long-term survival in various types of malignancies including esophageal cancer. SICs usually develop 7 to 10 days after esophagectomy and early diagnosis is believed to be difficult because systemic response by SICs is considered to be covered by surgical invasion. The aim of this study was to investigate whether serum C-reactive protein (CRP) on postoperative day (POD) 4 can be predictor of SICs after esophageal cancer surgery. Methods: The present study retrospectively examined 110 consecutive patients undergoing open thoracic esophagectomy after neoadjuvant chemotherapy for thoracic esophageal cancer between January 2011 and June 2015. All patients received perioperative care of enhanced recovery after surgery program with steroid therapy. SICs were defined as morbidity of grade III or more according to the Clavien-Dindo classification. Diagnostic accuracy was determined by measuring the area under the receiver operating characteristic curve (AUC). Clinical and laboratory parameters including CRP, all available before or within POD 4, were analyzed with univariate and multivariate logistic regression model to identify SICs. Results: Median age was 68 years. SICs were observed in 20 patients (18.2%). CRP on POD 4 had superior diagnostic accuracy for PICs (AUC 0.759; 95% confidence interval [CI], 0.652-0.866). Cut-off value for CRP was determined as 4.0 mg/dl which yielded a sensitivity of 70.0%, a specificity of 74.4% and a negative predictive value of 91.8% for the detection of SICs. Multivariate analysis identified CRP ≥ 4.0 mg/dl on POD 4 (odds ratio of 8.399 with 95% CI, 2.646–26.666) and three-field lymph node dissection (odds ratio of 4.658 with 95% CI, 1.306–16.619) as predictive factors for SICs after esophagectomy. Conclusions: CRP on POD 4 was an early indicator for serious infectious complications after esophageal cancer surgery, which could encourage imaging study to detect the focus or early intervention by antibiotics.
HIV charity criticises NHS England over “homophobic” PrEP statement
Authors: Gareth Iacobucci
Journal: BMJ (2016)

The HIV and Aids charity the Terrence Higgins Trust has accused NHS England of issuing an “intentionally provocative” and “homophobic” statement in response to a court ruling that it holds responsibility for commissioning pre-exposure prophylaxis (PrEP) for preventing HIV.

As a result of the High Court verdict on 2 August, NHS England said that it could now not confirm funding for other specialised …
Alemtuzumab-CHOP for Aggressive T Cell Lymphoma. A Phase II HOVON 69 Trial.
Authors: J.C. Kluin-Nelemans, Marinus Van Marwijk Kooij, P.J. Lugtenburg, Pierre W Wijermans, Willem JL Van Putten, Gustaaf W van Imhoff
Journal: Blood (2008)
Collaborative Data Project [C-DATA] of the Comprehensive Sickle Cell Centers Program.
Authors: Zora R. Rogers, Susan Lieff, Marsha McMurray, Carlton Dampier, Winfred C. Wang, Melanie Chelednik, George R. Buchanan, Kenneth I. Ataga, Laura M. De Castro, Matthew M. Heeney, Karen Kalinyak, Kim Smith-Whitley, Elliott Vichinsky, Ronald W. Helms
Journal: Blood (2006)
Loss of Function of GALNT2 Lowers High-Density Lipoproteins in Humans, Nonhuman Primates, and Rodents
Authors: Sumeet A. Khetarpal, Katrine T. Schjoldager, Christina Christoffersen, Avanthi Raghavan, Andrew C. Edmondson, Heiko M. Reutter, Bouhouche Ahmed, Reda Ouazzani, Gina M. Peloso, Cecilia Vitali, Wei Zhao, Amritha Varshini Hanasoge Somasundara, John S. Millar, YoSon Park, Gayani Fernando, Valentin Livanov, Seungbum Choi, Eric Noé, Pritesh Patel, Siew Peng Ho, Todd G. Kirchgessner, Hans H. Wandall, Lars Hansen, Eric P. Bennett, Sergey Y. Vakhrushev, Danish Saleheen, Sekar Kathiresan, Christopher D. Brown, Rami Abou Jamra, Eric LeGuern, Henrik Clausen, Daniel J. Rader
Journal: Cell Metabolism (2016)
Letter by Mezue et al Regarding Article, “Effect of Intensive Versus Usual Blood Pressure Control on Kidney Function Among Individuals With Prior Lacunar Stroke: A Post Hoc Analysis of the Secondary Prevention of Small Subcortical Strokes (SPS3) Randomized Trial”
Authors: Kenechukwu Mezue, Ritika Puri, Janani Rangaswami
Journal: Circulation (2016)
Abstract 507: Prediction of colorectal cancer recurrence with plasma miRNAs in the ColoCare Study
Authors: Brandon Dickinson, Scott V. Adams, Cornelia Ulrich, Kathy Vickers, Michelle Wurscher, William M. Grady, Karen W. Makar
Journal: Cancer Research (2016)

Abstract Background Colorectal cancer is the third most common cancer and third most common cause of cancer-related death in the US. Recurrence of primary tumors occurs in 10-40% of patients depending on tumor stage. Early detection of recurrence can increase the treatment options for those eligible for curative therapy, which may lead to better survival. We determined the levels of multiple different plasma miRNAs and assessed their association with clinical outcomes to identify specific miRNAs that may serve as predictors of CRC recurrence. Methods Colorectal cancer patients (age 18-80, newly diagnosed, stage I-IV) were recruited as part of the ColoCare Study in Seattle, Washington, between 2007 and 2011. Patients who had a blood sample taken &amp;gt;30 and &amp;lt;365 days after surgery and prior to any recurrence were included in the study (n = 83). Candidate miRNAs (miRNA 31, 141, 200b, 203, 16, 17-3p, 29a, 92a, 125b) were quantified using pre-designed TaqMan microRNA assays in triplicate assays. Plasma miRNA expression was normalized and expressed as copy number. Cox proportional hazards regression with robust standard errors was used to estimate hazard ratios of recurrence associated with level of each miRNA, adjusted for age, sex and tumor site and stratified by stage. No recurrences were observed in stage I patients. Statistical analyses were conducted separately for stages II-III and II-IV colorectal cancer. Results During a median of 1,067 days (33-2,254 days) follow-up after surgery, 20 recurrences were observed. In stage II-III patients, the presence of detectable circulating levels of miR-141 (HR = 4.30 [1.10-16.84]) or miR-203 (HR = 8.04 [1.39-46.4]) were associated with risk of recurrence. In stage II-IV patients, detectable miR-203 (HR = 3.52 [1.13-10.96]) was associated with recurrence, and elevated miR-29a levels were associated with greater risk of recurrence (HR: 1.80 per natural log unit, 95% CI: 1.06-3.04). Conclusion The present study identified multiple plasma miRNAs that may help to predict those patients at greatest risk of colorectal cancer recurrence. Our results require validation in order to determine the role for such plasma markers in clinical practice. Citation Format: Brandon Dickinson, Scott V. Adams, Cornelia Ulrich, Kathy Vickers, Michelle Wurscher, William M. Grady, Karen W. Makar. Prediction of colorectal cancer recurrence with plasma miRNAs in the ColoCare Study. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 507.
Clinical Features and Treatment Outcomes of Primary Testicular Lymphoma.
Authors: Byeong-Bae Park, Jong Gwang Kim, HyeonSeok Eom, Hyo Jin Kim, Hyuck Chan Kwon, Sung Yong Oh, Jung Hun Kang, Suk Joong Oh, Ho-Jin Shin, Cheolwon Suh, Jung Han Kim, Ho Young Kim, Sang Cheol Lee, Kihyun Kim, Chul W. Jung, Won Seog Kim
Journal: Blood (2006)
Abstract 1118: The role of Epstein-Barr virus-encoded miRNAs in ATM regulating DNA damage response in nasopharyngeal carcinoma
Authors: RAYMOND Wai-Ming LUNG, Tom Pok-Man Hau, Wing-Po Chak, Joanna Hung-Man Tong, Ken Hung-On Yu, Sai-Wah Tsao, Kevin Yuk-Lap Yip, Ka-Fai To, Kwok-Wai Lo
Journal: Cancer Research (2016)

Abstract Nasopharyngeal carcinoma (NPC) is an aggressive epithelial malignancy that has remarkably high incidence and mortality rates in Hong Kong and south China. It is well-known to be associated with Epstein-Barr virus (EBV) infection. Previous work in our team revealed that EBV-encoded miRNAs derived from the BARTs (miR-BARTs) were abundantly expressed in primary NPCs. They function in controlling apoptotic and immune responses in the infected cells during NPC development. Most recently, we attempted to use in silico method to predict cellular targets of miR-BARTs. Results suggested that there were multiple putative miR-BART binding sites on the 3’UTRs of an important DNA double-strand breaks (DSBs) repair gene, Ataxia-Telangiectasia-Mutant (ATM). The down-regulation of ATM mRNA and protein had also been demonstrated in our local primary NPC samples. Although the interaction of miR-BARTs on each suggested putative binding site had not been completely validated, our preliminary data indicated at least three EBV-encoded miRNAs (BART5-5p, BART9-3p and BART14-3p) were involved in repressing ATM expression in NPCs. They could work either alone or cooperatively to control ATM expression in transient assays. Notably, ectopic expression of these three miR-BARTs could successfully suppress γ irradiation-induced ATM activity in two EBV-negative cell lines, NP69 and HeLa. It had been reported that ATM null cells were defective to repair the DSB lesions and sensitive to the Poly(ADP-ribose) polymerase (PARP) inhibitor treatment in the presence of DNA-damaging agents. In contrast, the DSBs are effectively repaired in normal cells to retain genetic integrity. We believed that EBV infection, via miR-BARTs to reduce ATM activity and disrupt Homologous Recombination (HR) repair function, made the NPC cells sensitize to ionizing radiation and DNA-damaging agent treatment. Hence, the knowledge generated from this project is not only enhance our understanding of EBV biology, but also opens an avenue for the development of effective NPC therapies. Citation Format: RAYMOND Wai-Ming LUNG, Tom Pok-Man Hau, Wing-Po Chak, Joanna Hung-Man Tong, Ken Hung-On Yu, Sai-Wah Tsao, Kevin Yuk-Lap Yip, Ka-Fai To, Kwok-Wai Lo. The role of Epstein-Barr virus-encoded miRNAs in ATM regulating DNA damage response in nasopharyngeal carcinoma. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1118.
Treating Nonepileptic Seizures: Therapist Guide; Taking Control of Your Seizures: WorkbookTreating Nonepileptic Seizures, by W. Curt LaFrance, Jr., and Jeffrey Peter Wincze. New York, Oxford University Press, 2015, 192 pp., $55.00 (paperback).Taking Control of Your Seizures, by Joel M. Reiter, Donna Andrews, Charlotte Reiter, and W. Curt LaFrance, Jr. New York, Oxford University Press, 2015, 336 pp., $49.95 (paperback).
Authors: Brian Rothberg
Journal: American Journal of Psychiatry (2016)
Inferior Long-Term Outcome of Front-Line Hypomethylating Agent Compared to Supportive Care in Patients with Lower Risk Myelodysplastic Syndrome: Prosensity Score Matched Analysis
Authors: Sang Kyun Sohn, Joon Ho Moon, Ahn Jae Sook, Hyeoung Joon Kim, Moo kon Song, Ho-Jin Shin, Won Sik Lee, Sang Min Lee, Young Don Joo, Ho Sup Lee, Yang Soo Kim, Yoon Young Cho, Sung Hwa Bae
Journal: Blood (2014)