tbuckley/cabot-search huggingface dataset:
Effects of naloxone on patients with panic attacks
Authors: M R Liebowitz, J M Gorman, A J Fyer, D J Dillon, D F Klein
Journal: American Journal of Psychiatry (1984)

To determine whether endogenous opiates are involved in panic anxiety, the authors challenged 12 patients with panic attacks with intravenous naloxone hydrochloride alone or combined with sodium lactate. Naloxone did not produce panic attacks or alter responses to lactate.
Survivin expression in Ewing sarcoma family of tumors.
Authors: P. Hingorani, D. Azorsa, A. white-Collins, P. Dickman, M. Seidel
Journal: Journal of Clinical Oncology (2011)

9558 Background: Despite significant advances in treatment of pediatric sarcomas, the 5-year survival rates for metastatic and relapsed sarcomas remain dismal. Novel therapies are needed for such patients. Survivin, an inhibitor of apoptosis protein, is over expressed in several human cancers including lung, breast, colon, brain tumors, melanoma, neuroblastoma and soft tissue sarcomas and increased survivin expression is associated with reduced overall survival. The goal of the current study was to evaluate the role of survivin in Ewing sarcoma (ES), the second most common pediatric bone sarcoma. Methods: The goal of this study was to evaluate the expression of survivin in ES cell lines and tissue samples and to correlate the degree of expression with conventional clinical parameters. Survivin protein expression was determined in ES cell lines using Western Blot analysis. To examine a functional role for survivin in growth of ES cells, cells were treated with four siRNAs targeting survivin and cell growth and viability was assessed following survivin inhibition with the siRNAs. Immunohistochemical analysis was performed on paraffin-embedded patient samples using an anti-survivin antibody. Staining was scored from 0-3 depending on the percentage of positive cells. The degree of expression was correlated with clinical parameters such as tumor site, presence or absence of metastases, event-free and overall survival. Results: Survivin is overexpressed in a panel of ES cell lines. Gene silencing of survivin in the Ewing's sarcoma cell line TC-71 decreased cell growth by more than 50% for each survivin siRNA compared to non-silencing control siRNA. Survivin is overexpressed in majority of the ES tumor samples with a higher degree of expression noted in patients with metastatic disease and patients with relapsed disease. Further statistical analysis is currently underway to confirm the effect on survival rates. Conclusions: Our results show that survivin is overexpressed in ES cell lines and patient samples. Further, it plays an important role in cell growth and viability in-vitro. Higher degree of expression in patients with advanced and relapsed disease may indicate that survivin plays an imporatant role in ES pathogenesis and overall patient outcome.
“Hybrid” Type Cells in Combined Cultures of Two Different Mammalian Cell Strains
Authors: G. Barski, S. Sorieul, Fr. Cornefert
Journal: JNCI Journal of the National Cancer Institute (1961)

Cells of a high-cancer N1 line derived from NCTC 2472 clone of mouse fibroblasts and cells of N2 or N2T′ lines derived from low-cancer NCTC 2555 clone of the same drigin were cultivated together in vitro. The evolution of mixed-cell populations was compared with the behavior of component cells in separate cultures. Separately cultivated, the cell lines kept their essential characteristics fairly stable. N1 cells maintained a karyotype with a modal number of nearly 55 telocentric chromosomes—one extra long. N2 lines had modal chromosome numbers of 62 and 58 chromosomes, 13 biarmed. Higher polyploids in pure cultures were recognized as doubles or quadruples of stemline cells. In mixed cultures the proportion of N1 cells progressively decreased until they almost completely disappeared. At the 3d month, a new type of cell designated “M” appeared, which later reached the proportion of one third to one fourth of the whole cell population. The M cells had many “hybrid” chromosomal and biological characteristics. They showed a modal number of 115 to 116 chromosomes with 9 to 15 metacentrics, frequently with one extra long telocentric. M cells from mixed populations and secondarily isolated M-type cell clones produced, in isologous mice, malignant tumors in which M cells could be identified. The possible origin of the “hybrid” type cells is discussed.
Abnormal cortisol suppression in bipolar patients with simultaneous manic and depressive symptoms
Authors: R R Krishnan, A A Maltbie, J R Davidson
Journal: American Journal of Psychiatry (1983)

The authors studied the cortisol response to 1 mg of dexamethasone in 10 patients who simultaneously manifested manic and depressive symptoms. All patients showed some evidence that normal cortisol suppression was lacking. When 3 patients were randomly retested after recovery, the results suggested the normalization of suppression.
OP0255 MMP13 is Transcriptionally Repressed by the HIF1α/β-Catenin Interaction in Chondrocytes and Osteoarthritis in Mice
Authors: W. Bouaziz, J. Sigaux, D. Modrowski, C. Marty, S. Provot, H.-K. Ea, M. Cohen-Solal, E. Hay
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> Activation of Wnt/β-catenin pathway triggers chondrocyte catabolism and MMP13 activation that contribute to osteoarthritis (OA). The mechanism of down-regulation of Wnt/β-catenin pathway in cartilage homeostasis is unknown. Because chondrocytes are in a hypoxic environment that is lost in OA, we speculated that Hypoxia Inducible Factor 1α (HIF1α) inhibits Wnt signaling and chondrocyte catabolism. <h3>Objectives</h3> We here investigated the interaction of β-catenin/TCF4 and the molecular regulation of Mmp-13 in articular chondrocytes and in OA mice. <h3>Methods</h3> Primary murine chondrocytes from WT and ΔHIF1α were cultured with Wnt3a in normoxic (21% O<sub>2</sub>) and hypoxic (1% O<sub>2</sub>) conditions and analyzed the expression of catabolic markers. The binding of TCF4 to Mmp-13 regulatory regions was assessed in Chip assay in both conditions. To determine the impact of the interaction of Wnt and HIF1α in healthy cartilage and in OA, ΔHIF1α<sup>chon</sup> and fl/fl HIF1α underwent DMM and received articular injection of PKF 118-310, an inhibitor of β-catenin and TCF4 interaction. <h3>Results</h3> Hypoxia prevented the increase in Mmp-13 and the decrease in Col2 and ACAN expression induced by Wnt. Blocking HIF1α by siRNA or cre-recombinase enhanced the expression of Mmp-13, but not Col2 expression suggesting that HIF1α regulated catabolic rather than anabolic genes. In hypoxic chondrocytes, Chip assay revealed that HIF1α lowered β-catenin/TCF4 transcriptional activity and the expression of Mmp13. Indeed, HIF1α interacted with β-catenin which displaced TCF4 from <i>Mmp13</i> regulatory sequences. Moreover, DMM resulted in decreased HIF1α expression in articular cartilage of control mice. Furthermore, cartilage lesions were higher in ΔHIF1α<sup>chon</sup> mice submitted to DMM along with higher expression of β-catenin and Mmp13. Local administration of PKF 118-310 in ΔHIF1α<sup>chon</sup> reduced Mmp13 expression and prevented cartilage lesions. These results showed that HIF1α limited cartilage breakdown by blocking the binding of β-catenin to TCF4 subsequently leading to a lower Mmp-13 activation. <h3>Conclusions</h3> We show here that the HIF1α/β-catenin complex acts as a negative regulator of Wnt signaling responsible for Mmp13 transcription in chondrocytes and in mice osteoarthritic joints. Therefore, targeting the HIF1α/β-catenin interaction is a new approach to reduce chondrocyte catabolism and prevent from osteoarthritis. <h3>Disclosure of Interest</h3> None declared
Intrauterine device removal from the sigmoid colon during a screening colonoscopy
Authors: Ana García-Rodríguez, Marco Álvarez, Agustin Seoane
Journal: Endoscopy (2015)

An intrauterine device (IUD) is a highly effective method of contraception and is associated with few side effects; however, uterine perforation and migration of the IUD to adjacent organs including the colon have been reported.
AB1074 Utility of the Stricter Remission Criteria for Assessing the Absence of Subclinical Activity in Rheumatoid Arthritis
Authors: M.V. Martire, D. Puente Trigo, V.M. Duarte, L. Marino Claverie, G. Amaya Crespo, M. Manzano, L. Carlevaris, A. Secco, M. Mamani, L. Santiago
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> It has been seen that patients who achieve remission measured by DAS28 persist with subclinical synovitis on hands and toes evaluated by ultrasound. In recent times stricter criteria as the CDAI, SDAI and ACR EULAR Boolean have been proposed. <h3>Objectives</h3> To evaluate the stricter criteria as diagnostic test with ultrasound considered as reference test or gold standard in patients in remission for DAS28. <h3>Methods</h3> We evaluated consecutive patients in remission by DAS28 less than 2,6. Strict criteria were applied. A rheumatologist specializing in musculoskeletal ultrasound, who was unaware of the clinical status and laboratory activities, performed ultrasonography on hands and and toes. Systematic evaluation was performed on grayscale and Doppler technique with multifrequency linear transducer (10 -18 MHz) in longitudinal and transverse sections of radiocarpal joint, second and third metacarpophalangeal, second and third proximal interphalangeal in both hands, second to fifth metatarsophalangeal joint, bilateral. It was considered as cases, synovitis absence grade II-III and/or Power Doppler II-III using OMERACT9s definitions. We defined controls as the ones with at least one of these signs of activity. We consider positive diagnosis test to remission status for different stricter criteria. Sensitivity, specificity, positive likelihood ratio, positive predictive value (PPV) and negative predictive value (NPV). <h3>Results</h3> 100 scans were performed in 100 patients, 80% were women (n=80) and the mean age 52.7 SD 12. There were no differences in age, sex, duration of disease, treatment, or RF and anti CCP positivity between cases and controls. Of the ultrasound screening 58 didn9t have signs of activity and 42 had synovitis and/or PD grade II-III. Table 1 shows the status of activity and Table 2 shows the results. <h3>Conclusions</h3> We found that in this population, which is in remission for DAS28, none of the three criteria is of great value to detect absence of activity using ultrasound as gold standard. <h3>References</h3> Felson DT, Smolen JS, Wells G, Zhang B, van Tuyl LH, Funovits J, et al. American College of Rheumatology; European League Against Rheumatism. American College of Rheumatology/European League Against Rheumatism provisional definition of remission in rheumatoid arthritis for clinical trials. Arthritis Rheum. 2011; 63:573-86. <h3>Disclosure of Interest</h3> None declared
Human Immunodeficiency Virus-1 env Impairs Fcγ Receptor-Mediated Phagocytosis Via a Cyclic Adenosine Monophosphate-Dependent Mechanism
Authors: Christian A. Thomas, Ofra K. Weinberger, Benedikt L. Ziegler, Steven Greenberg, Ira Schieren, Samuel C. Silverstein, Joseph El Khoury
Journal: Blood (1997)
Updated analysis of weekly nab-Paclitaxel (P)+ Gemcitabine (G) with metastatic pancreatic cancer (MPC): Phase I / II trial
Authors: Yasushi Omuro, Hideki Ueno, Masafumi Ikeda, Makoto Ueno, Nobumasa Mizuno, Tatsuya Ioka, Takako Nakajima, Junji Furuse
Journal: Annals of Oncology (2015)
THU0262 Multifocal Infectious Spondylodiscitis in Central Tunisia: Clinical and Therapeutic Features
Authors: Z. Alaya, M. Bouzaouache, K. Baccouche, H. Zaghouani, S. Belghali, D. Amri, H. Zeglaoui, E. Bouajina
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> The infectious spondylodiscitis is the location of a microbial agent to the body of the vertebra associated with disc disease. It is called multifocal when the spinal involvement of several floors is not contiguous. It is seen especially in immunocompromised patients. <h3>Objectives</h3> The objective of our study was to describe the clinical, biological, radiological and therapeutic aspects of multifocal infectious spondylodiscitis. <h3>Methods</h3> This is a retrospective study of 84 cases of infectious spondylodiscitis collected in department of Rheumatology over a period of 15 years [1999-2014]. Multifocal infectious spondylodiscitis was observed in 6 cases. <h3>Results</h3> Our population consists of 4 men and 2 women. The mean age was 52 years [36-64]. One patient was diabetic. The tuberculous origin was retained in 5 cases and the Brucella origin in one case. The mean disease duration was 5 months [1-12 months]. The start mode was progressive in all cases. The circumstances of discovery were inflammatory spinal pain in all cases associated to general signs such alteration of the general state and night sweats in 5 cases. Radicular pain was observed in 4 cases. Motor deficit was objectified in 4 cases. An array of spinal compression occurred in 2 cases. The biological inflammatory syndrome was observed in all cases. X-ray showed disc space narrowing in 6 cases and erosion of the vertebral endplates in 3 cases. MRI performed in 5 cases showed a typical appearance of infectious spondylodiscitis in all cases, an epiduritis in one case, an infiltration of the soft parts in 5 cases and spinal cord compression in 2 cases. Spondylodiscitis interested lumbar spine in one case, thoracic and lumbar spine in 3 cases and lumbar and cervical spine in 2 cases. All patients benefited of disco-vertebral puncture biopsy. Histological examination was specific for tuberculosis in 3 cases. The tuberculous origin was retained in 2 cases based on clinical and radiological data. The Brucella serology was positive in one case. All patients with tuberculous spondylodiscitis were put on anti tuberculosis treatment. The patient with brucella spondylodiscitis was put on association of cyclin and rifampicin. The evolution was unfavorable in one case with persistence of neurological sequelae. <h3>Conclusions</h3> Multifocal infectious spondylodiscitis can occur in immunocompetent individuals. The causative organisms are dominated by tuberculosis. Treatment should be initiated as soon as possible to avoid neurological sequelae especially. <h3>Disclosure of Interest</h3> None declared
THU0305 Fatigue is Correlated to Pain Mechanism Rather than to Inflammatory Load in Patients with Rheumatoid Arthritis: A Descriptive Cross-Sectional Danbio Registry Survey
Authors: A.W. Christensen, S. Rifbjerg-Madsen, R. Christensen, M. Hetland, H. Bliddal, L.E. Kristensen, B. Danneskiold-Samsø, K. Amris
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> In patients with rheumatoid arthritis (RA), patient reported outcomes (PROs) are sometimes reported to be refractory to therapies targeting anti-inflammatory pathways, which may be due to influence of central pain mechanisms. The painDETECT Questionnaire (PDQ), which is a validated questionnaire developed to detect neuropathic pain, assigns a score to the patient. Since its development the PDQ has also been used to link the pain phenotypic similarities of a neuropathic pain and central sensitization in osteoarthritis and fibromyalgia, and may be used as well in RA. <h3>Objectives</h3> To examine whether fatigue is closer associated to the PDQ-score than CRP in patients with RA. <h3>Methods</h3> An electronic version of the PDQ was added to the DANBIO touchscreens in the waiting rooms at 22 of 24 departments of Rheumatology in Denmark for a period of six months. Patient consent was obtained and corresponding data on patients diagnosed with RA in the DANBIO database were extracted. A PDQ score &gt;18 was interpreted as an indication of predominant central pain mechanisms, 13-18 as unclear and &lt;13 as nociceptive pain. Interactions between strata were based on Kruskal-Wallis test and correlations based on Spearman correlation coefficients. <h3>Results</h3> Out of 15978 patients invited to participate in the survey, 9024 were diagnosed with RA. Of these, 4387 patients agreed to fill in the PDQ and 3826 patients completed it. Patients with higher PDQ-scores had higher VAS-fatigue, DAS28, VAS-global health and more tender and swollen joints (Table 1). VAS-fatigue correlated moderately with the PDQ-score (Rho=0.48), but not with CRP (Rho=0.09) as shown in Table 2. <h3>Conclusions</h3> These results suggest that fatigue, in patents with RA, shows a closer association with PDQ (indicator of clinical features of augmented central pain processing) than with CRP (indicator of inflammatory load). Disease models other than peripheral inflammatory driven models are probably needed to fully understand the mechanisms underlying individual differences in pain characteristics and might allow for a more individual approach to treatment. <h3>Acknowledgements</h3> This study is supported financially by The Oak Foundation and Selsbjerg Holding. The authors would like to acknowledge the participating Departments of Rheumatology, DANBIO and Zitelab for their assistance. <h3>Disclosure of Interest</h3> None declared
Suppressive as compared with Analgesic Hormonal Therapy in Patients with Rheumatoid Arthritis
Authors: L. W. Kinsell
Journal: Annals of the Rheumatic Diseases (1954)

<h3>Abstract</h3> To identify <i>cis</i>-regulatory elements (CREs) and motifs of TF binding is an important step in understanding the regulatory functions of TF binding and gene expression. The lack of experimentally determined and computationally inferred data means that the genome-wide CREs and TF binding sites (TFBs) in filamentous fungi remain unknown. ATAC-seq is a technique that provides a high-resolution measurement of chromatin accessibility to Tn5 transposase integration. In filamentous fungi, the existence of cell walls and the difficulty in purifying nuclei have prevented the routine application of this technique. Herein, we modified the ATAC-seq protocol in filamentous fungi to identify and map open chromatin and TF-binding sites on a genome-scale. We applied the assay for ATAC-seq among different <i>Aspergillus</i> species, during different culture conditions, and among TF-deficient strains to delineate open chromatin regions and TFBs across each genome. The syntenic orthologues regions and differential changes regions of chromatin accessibility were responsible for functional conservative regulatory elements and differential gene expression in the <i>Aspergillus</i> genome respectively. Importantly, 17 and 15 novel transcription factor binding motifs that were enriched in the genomic footprints identified from ATAC-seq data of <i>A. niger</i>, were verified in vivo by our artificial synthetic minimal promoter system, respectively. Furthermore, we first confirmed the strand-specific patterns of Tn5 transposase around the binding sites of known TFs by comparing ATAC-seq data of TF-deficient strains with the data from a wild-type strain.
Soluble interleukin-2 receptors in the sera of patients with hairy cell leukemia: relationship with the effect of recombinant alpha-interferon therapy on clinical parameters and natural killer in vitro activity
Authors: M Chilosi, G Semenzato, G Cetto, A Ambrosetti, L Fiore-Donati, G Perona, G Berton, M Lestani, A Scarpa, C Agostini
Journal: Blood (1987)
ECG changes during haloperidol and pimozide treatment of Tourette's disorder
Authors: G Fulop, R A Phillips, A K Shapiro, J A Gomes, E Shapiro, J W Nordlie
Journal: American Journal of Psychiatry (1987)

The ECG QTc interval was significantly prolonged by pimozide but not haloperidol or placebo in a randomized clinical trial with 40 patients with Tourette's disorder. However, no adverse cardiac effects or differences in rate, rhythm, or waveform were found.
Letter by Boulon and Constans Regarding Article, “Relation of Nailfold Capillaries and Autoantibodies to Mortality in Patients With Raynaud Phenomenon”
Authors: Carine Boulon, Joël Constans
Journal: Circulation (2016)

Mueller et al 1 found a relation between nailfold capillaries and antinuclear autoantibodies and mortality in patients with Raynaud phenomenon.Both methods are recommended in Raynaud phenomenon to detect patients at risk to have connective tissue disease but especially systemic sclerosis.Raynaud phenomenon usually is the first clinical sign of systemic sclerosis, and systemic sclerosis is associated with increased mortality.The authors found a relationship between capillaroscopy or antinuclear autoantibodies and mortality only in females but not in males.Capillaroscopy as presented by the authors raises 3 problems.The first one is the high magnification that was used (×250-300) versus ×50 to ×100 for many vascular specialists.Then, only a very small part of the capillary field was observed, and the authors do not indicate whether the central part was examined for each finger or whether the place of the most important abnormalities was considered.The last problem is that the findings of capillaroscopy were classified as abnormal if any among 10 abnormalities was found.However, the authors recorded many aspecific and frequent abnormalities, such as reduced density, tortuous capillaries, hemorrhages (often related to finger trauma in men), or capillary edema.Abnormal capillaroscopic findings are usually divided into sclerodermic landscape or aspecific abnormalities. 2 We believe that the relation between sclerodermic landscape and mortality might be stronger than the relation between aspecific capillaroscopic abnormalities and mortality, and this relation might be significant in males as well.
Predictors of response: new versus old?
Authors: B Vikram
Journal: Journal of Clinical Oncology (1994)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1994.12.5.1102 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 8164034 Predictors of response: new versus old? B VikramxB VikramSearch for articles by this author https://doi.org/10.1200/JCO.1994.12.5.1102 PDF "Predictors of response: new versus old?." Journal of Clinical Oncology, 12(5), p. 1102© 1994 by American Society of Clinical Oncology