tbuckley/cabot-search huggingface dataset:
More Than 15 Years of Experience with Intradetrusor OnabotulinumtoxinA Injections for Treating Refractory Neurogenic Detrusor Overactivity: Lessons to Be Learned
Authors: Lorenz Leitner, Sharmistha Guggenbühl-Roy, Stephanie C. Knüpfer, Matthias Walter, Marc P. Schneider, Jure Tornic, Ulla Sammer, Ulrich Mehnert, Thomas M. Kessler
Journal: European Urology (2016)
Identiflcatlon and Tumor-Associated Venereal Sarcoma Physicochemical Characterization of a Antigen From Canine Transmissible&lt;xref ref-type="fn" rid="fn2"&gt;2&lt;/xref&gt;&lt;xref ref-type="fn" rid="fn3"&gt;3&lt;/xref&gt;&lt;xref ref-type="fn" rid="fn4"&gt;4&lt;/xref&gt;
Authors: Thomas J. Palker, Tsu-Ju Thomas Yang
 6
Journal: JNCI Journal of the National Cancer Institute (1981)

A tumor antigen associated wtih canine transmissible venereal sarcoma (CTVS) has been identified and partially characterized. The antigen was demonstrated in 3-M KCI and saline extracts of washed CTVS cells. Rabbit anti-CTVS antisera absorbed wih glutaraldehyde cross-linked normal dog serum and pooled homogenates of canine spleen, thymus, lymph node, and liver were used to identify the antigen. The specificity of the rabbit anti-CTVS antiserum for the CTVS antigen was established by use of immunodiffusion, two-dimensional electrophoresis, and the enzyme-linked immunosorbent assay (ELISA). Extracted CTVS antigen was fractionated by Sephadex G-200 chromatography, and the antigen factions were identified with the use of absorbed rabbit anti-CTVS antiserum and the ELISA technique. Antigen activity was detected in samples with estimated molecular weights greater than 70,000 daltons. Antigen fractions reacted with absorbed anit-CTVS antiserum to form a single precipitation band in immunodiffusion studies. Extraction of CTVS antigen with 3 M KCl and saline in the presence of a protease inhibitor did not significantly alter the antigen activity or molecular weight of the CVTS antigen. Cytoplasmic and nucleolar fluorescence were observed in an indirect immunofluorescence test with the use of acetone-fixed CTVS cells and absorbed anti-CTVS antiserum. The CTVs antigen activity was greatly reduced by trypsin digestion, by incubation for 1 hour at 65 degrees C, and by exposure to pH 2.8, 4.0, and 11.0 for 1 hour each.
Abstract 2411: Non-invasive analysis for T790M mutations of EGFR using a selective amplification method
Authors: Shingo Nishikawa, Hideharu Kimura, Hayato Koba, Taro Yoneda, Takashi Sone, Chris Booth, Andrew Webb, Kazuo Kasahara
Journal: Cancer Research (2015)

Abstract Epidermal growth factor receptor (EGFR) T790M mutation is associated with EGFR tyrosine kinase inhibitors (EGFR-TKIs) resistance in non-small cell lung cancer (NSCLC). However, tissue availability limits the genotyping of EGFR T790M mutation in a clinical setting. The aims of this study are to develop a blood-based, non-invasive approach to detecting the EGFRT790M mutation in advanced NSCLC patients, using PointMan™ EGFR DNA Enrichment Kit, which is a novel method for selective amplification of genotype specific sequences. Methods: Blood samples were collected from NSCLC patients with activating EGFR mutations, who were resistant to EGFR-TKIs treatment. EGFR T790M mutations in plasma DNA were detected using the kit. The concentrations of plasma DNA were determined using quantitative real-time PCR. Results: 21 (87.5%) of the patients had EGFR T790M mutations in their plasma DNA as detected using the kit. In all 6 cases detected T790M mutations from tumor tissues, the T790M mutations ware detected also in plasma DNA. The concentrations of plasma DNA were higher in patients with T790M mutations than without the mutations. Conclusions: The PointMan™ is an easily and useful method for determining the plasma EGFR T790M mutation status. Citation Format: Shingo Nishikawa, Hideharu Kimura, Hayato Koba, Taro Yoneda, Takashi Sone, Chris Booth, Andrew Webb, Kazuo Kasahara. Non-invasive analysis for T790M mutations of EGFR using a selective amplification method. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2411. doi:10.1158/1538-7445.AM2015-2411
Laminopathies disrupt epigenomic developmental programs and cell fate
Authors: Jelena Perovanovic, Stefania Dell’Orso, Viola F. Gnochi, Jyoti K. Jaiswal, Vittorio Sartorelli, Corinne Vigouroux, Kamel Mamchaoui, Vincent Mouly, Gisèle Bonne, Eric P. Hoffman
Journal: Science Translational Medicine (2016)

Mutations in lamin A/C and emerin change myogenic cell fate by disrupting heterochromatin tethering to the nuclear envelope.
Sentinel Node in Oral Cancer
Authors: Girolamo Tartaglione, Sandro J. Stoeckli, Remco de Bree, Clare Schilling, Geke B. Flach, Vivi Bakholdt, Jens Ahm Sorensen, Anders Bilde, Christian von Buchwald, Georges Lawson, Didier Dequanter, Pedro M. Villarreal, Manuel Florentino Fresno Forcelledo, Julio Alvarez Amezaga, Augusto Moreira, Tito Poli, Cesare Grandi, Maurizio Giovanni Vigili, Michael O’Doherty, Davide Donner, Elisabeth Bloemena, Siavash Rahimi, Benjamin Gurney, Stephan K. Haerle, Martina A. Broglie, Gerhard F. Huber, Annelise l. Krogdah, Lars R. Sebbesen, Edward Odell, Luis Manuel Junquera Gutierrez, Luis Barbier, Joseba Santamaria-Zuazua, Manuel Jacome, Marie-Cecile Nollevaux, Emma Bragantini, Philippe Lothaire, Enrico M. Silini, Enrico Sesenna, Giles Dolivet, Romina Mastronicola, Agnes Leroux, Isabel Sassoon, Philip Sloan, Patrick M. Colletti, Domenico Rubello, Mark McGurk
Journal: Clinical Nuclear Medicine (2016)

Nuclear imaging plays a crucial role in lymphatic mapping of oral cancer. This evaluation represents a subanalysis of the original multicenter SENT trial data set, involving 434 patients with T1-T2, N0, and M0 oral squamous cell carcinoma. The impact of acquisition techniques, tracer injection timing relative to surgery, and causes of false-negative rate were assessed.Three to 24 hours before surgery, all patients received a dose of Tc-nanocolloid (10-175 MBq), followed by lymphoscintigraphy. According to institutional protocols, all patients underwent preoperative dynamic/static scan and/or SPECT/CT.Lymphoscintigraphy identified 723 lymphatic basins. 1398 sentinel lymph nodes (SNs) were biopsied (3.2 SN per patient; range, 1-10). Dynamic scan allowed the differentiation of sentinel nodes from second tier lymph nodes. SPECT/CT allowed more accurate anatomical localization and estimated SN depth more efficiently. After pathological examination, 9.9% of the SN excised (138 of 1398 SNs) showed metastases. The first neck level (NL) containing SN+ was NL I in 28.6%, NL IIa in 44.8%, NL IIb in 2.8%, NL III in 17.1%, and NL IV in 6.7% of positive patients. Approximately 96% of positive SNs were localized in the first and second lymphatic basin visualized using lymphoscintigraphy. After neck dissection, the SN+ was the only lymph node containing metastasis in approximately 80% of patients.Best results were observed using a dynamic scan in combination with SPECT/CT. A shorter interval between tracer injection, imaging, and surgery resulted in a lower false-negative rate. At least 2 NLs have to be harvested, as this may increase the detection of lymphatic metastases.
Sodium Cyanate as a Potential Treatment for Sickle-Cell Disease
Authors: Peter N. Gillette, Charles M. Peterson, Yang S. Lu, Anthony Cerami
Journal: New England Journal of Medicine (1974)

Thirty-one patients with sickle-cell disease, seven to 49 years of age, were given the antisickling drug sodium cyanate (10 to 35 mg per kilogram of body weight per day by mouth) for six to 18 months. Carbamylation of the amino-terminal valine of hemoglobin was dose-related and ranged from 0.03 to 0.67 moles of carbamyl group per mole of hemoglobin tetramer. No toxic effects were observed at a dosage of 35 mg per kilogram per day or less. Occasional gastrointestinal distress and drowsiness occurred. The previously demonstrated ability of sodium cyanate to diminish the hemolytic rate was confirmed. The cyanate-induced increase in hemoglobin concentration (3 to 38 per cent) was dose related and resulted from decreased red-cell destruction. A retrospective analysis of the patients' records revealed a statistically significant decrease in the mean frequency of crises (from 3.6 to 2.1 crises per year) between two groups of patients with low (<0.3 mole of carbamyl group per mole of hemoglobin tetramer) and high (≥0.3 mole) carbamylatlon. Although these studies do not establish the long-term safety and clinical usefulness of sodium cyanate they indicate its potential value and justify large-scale trials. (N Engl J Med 290:654–660, 1974)
Demonstration of Adrenal Metastases by Adrenal Venography
Authors: Stewart R. Reuter
Journal: New England Journal of Medicine (1968)

Adrenal metastases were detected in five patients by means of adrenal venography. It is possible to demonstrate very small tumor nodules by the method, but because they can be identified only by displacement of normal adrenal veins, no criteria exist for differentiating a solitary metastasis from an adrenal adenoma.
Homoharringtonine In Combination With Cytarabine and Etoposide For Induction Therapy In Patients With De Novo Acute Myelogenous Leukemia
Authors: Jingsong He, Li Li, Jingjing Zhu, Yi Zhao, Wenjun Wu, Yanlong Zheng, Gaofeng Zheng, Weiyan Zheng, Xiaoli Zhu, He Huang, Xiujin Ye
Journal: Blood (2013)
Obsessive–Compulsive and Related Disorders
Authors: Michael A. Jenike
Journal: New England Journal of Medicine (1989)

"I know it's stupid. I feel like a crazy person, but I know that I'm not crazy!" These are typical statements from a patient with obsessive–compulsive disorder who for the past 10 years has spent more than six hours a day washing her hands, although she recognizes that it is senseless. Other patients are compelled to check and recheck that a stove is off or the doors locked, for hours every day. An internist calls the laboratory 15 times to confirm that he heard the white-cell count correctly. After hours of viewing, a pathologist cannot decide whether a slide reveals . . .
Comparative Effectiveness and Safety of Anti–Tumor Necrosis Factor Agents in Biologic-Naive Patients With Crohn’s Disease
Authors: Siddharth Singh, Herbert C. Heien, Lindsey R. Sangaralingham, Stephanie R. Schilz, Michael D. Kappelman, Nilay D. Shah, Edward V. Loftus
Journal: Clinical Gastroenterology and Hepatology (2016)
Biomaterial property-controlled stem cell fates for cardiac regeneration
Authors: Yanyi Xu, Jianjun Guan
Journal: Bioactive Materials (2016)

Myocardial infarction (MI) affects more than 8 million people in the United States alone. Due to the insufficient regeneration capacity of the native myocardium, one widely studied approach is cardiac tissue engineering, in which cells are delivered with or without biomaterials and/or regulatory factors to fully regenerate the cardiac functions. Specifically, in vitro cardiac tissue engineering focuses on using biomaterials as a reservoir for cells to attach, as well as a carrier of various regulatory factors such as growth factors and peptides, providing high cell retention and a proper microenvironment for cells to migrate, grow and differentiate within the scaffolds before implantation. Many studies have shown that the full establishment of a functional cardiac tissue in vitro requires synergistic actions between the seeded cells, the tissue culture condition, and the biochemical and biophysical environment provided by the biomaterials-based scaffolds. Proper electrical stimulation and mechanical stretch during the in vitro culture can induce the ordered orientation and differentiation of the seeded cells. On the other hand, the various scaffolds biochemical and biophysical properties such as polymer composition, ligand concentration, biodegradability, scaffold topography and mechanical properties can also have a significant effect on the cellular processes.
Case 3-2003
Authors: Jonathan S. Coblyn, Robert T. McCluskey
Journal: New England Journal of Medicine (2003)

Presentation of CaseA 36-year-old, right-handed man was admitted to the hospital because of seizures and severe hypertension.The patient had an 18-year history of intravenous drug abuse, including abuse of heroin and cocaine with needle sharing. One year before admission, he discontinued his use of illicit drugs and began to feel vaguely unwell. Three months before admission, tingling developed in the left toes and progressed to numbness in the foot; these symptoms were accompanied by recurrent vomiting, night sweats, intermittent diarrhea, abdominal pain, subjective fever, and a weight loss of 16 kg. He noted erythematous lumps over the shins . . .
Multinational Home Use of Closed-Loop Control Is Safe and Effective
Authors: Stacey M. Anderson, Dan Raghinaru, Jordan E. Pinsker, Federico Boscari, Eric Renard, Bruce A. Buckingham, Revital Nimri, Francis J. Doyle, Sue A. Brown, Patrick Keith-Hynes, Marc D. Breton, Daniel Chernavvsky, Wendy C. Bevier, Paige K. Bradley, Daniela Bruttomesso, Simone Del Favero, Roberta Calore, Claudio Cobelli, Angelo Avogaro, Anne Farret, Jerome Place, Trang T. Ly, Satya Shanmugham, Moshe Phillip, Eyal Dassau, Isuru S. Dasanayake, Craig Kollman, John W. Lum, Roy W. Beck, Boris Kovatchev
Journal: Diabetes Care (2016)

OBJECTIVE To evaluate the efficacy of a portable, wearable, wireless artificial pancreas system (the Diabetes Assistant [DiAs] running the Unified Safety System) on glucose control at home in overnight-only and 24/7 closed-loop control (CLC) modes in patients with type 1 diabetes. RESEARCH DESIGN AND METHODS At six clinical centers in four countries, 30 participants 18–66 years old with type 1 diabetes (43% female, 96% non-Hispanic white, median type 1 diabetes duration 19 years, median A1C 7.3%) completed the study. The protocol included a 2-week baseline sensor-augmented pump (SAP) period followed by 2 weeks of overnight-only CLC and 2 weeks of 24/7 CLC at home. Glucose control during CLC was compared with the baseline SAP. RESULTS Glycemic control parameters for overnight-only CLC were improved during the nighttime period compared with baseline for hypoglycemia (time &amp;lt;70 mg/dL, primary end point median 1.1% vs. 3.0%; P &amp;lt; 0.001), time in target (70–180 mg/dL: 75% vs. 61%; P &amp;lt; 0.001), and glucose variability (coefficient of variation: 30% vs. 36%; P &amp;lt; 0.001). Similar improvements for day/night combined were observed with 24/7 CLC compared with baseline: 1.7% vs. 4.1%, P &amp;lt; 0.001; 73% vs. 65%, P &amp;lt; 0.001; and 34% vs. 38%, P &amp;lt; 0.001, respectively. CONCLUSIONS CLC running on a smartphone (DiAs) in the home environment was safe and effective. Overnight-only CLC reduced hypoglycemia and increased time in range overnight and increased time in range during the day; 24/7 CLC reduced hypoglycemia and increased time in range both overnight and during the day. Compared with overnight-only CLC, 24/7 CLC provided additional hypoglycemia protection during the day.
The Nurse Shortage
Authors: Linda H. Aiken, Connie Flynt Mullinix
Journal: New England Journal of Medicine (1987)

The proportion of vacant positions for registered nurses in hospitals doubled between September 1985 and December 1986,1 reaching the levels of the last national nursing shortage of 1979. Current reports of vacancies are perplexing in the light of the size of the nation's supply of nurses. The output of nurses has doubled over the past 30 years, greatly exceeding the population growth, and licensed registered nurses now number 2.1 million. Between 1977 and 1984 alone, the number of employed nurses increased by 55 percent, as compared with an 8 percent growth in population.2 Intuitively, it would seem that an increased . . .
Serum level monitoring of thiothixene in schizophrenia: acute single- dose levels at fixed doses
Authors: J A Yesavage, J Becker, P D Werner, M J Mills, C A Holman, R Cohn
Journal: American Journal of Psychiatry (1982)

After establishing a strong correlation between steady-state thiothixene levels and levels drawn 2.5 hours after a 20-mg test dose, the authors examined the correlation between clinical improvement and serum levels drawn 2.5 hours after a single 20-mg test dose of thiothixene. For 30 male schizophrenic inpatients, improvement on the Brief Psychiatric Rating Scale was significantly correlated with the single test-dose levels. Serum levels were also positively correlated with age, which may explain the tendency of elderly patients to require lower neuroleptic doses and to exhibit a higher incidence of side effects. The results suggest that acute single-dose levels may be useful in predicting clinical response to thiothixene.
ATPS-69POLYDOTS, A NOVEL NANOMICELLE DRUG DELIVERY SYSTEM, FOR TARGETED THERAPY OF BRAIN TUMORS
Authors: Jihong Xu, Alessandra Welker, Jose Otero, J. Brad Elder, Christine Beattie, Mark Calhoun, Metin Gurcan, Jessica O. Winter, Vinay Puduvalli
Journal: Neuro-Oncology (2015)

Standard therapies have limited success against malignant primary brain tumors such as glioblastoma (GB) raising the need for novel therapies that can efficiently transgress the blood brain barrier (BBB), target tumor and provide sustained drug release. Nanoparticle delivery systems which can bypass the BBB and target nervous system tumor cells allowing combined delivery of therapeutic agents with unique advantages compared to conventional agents. We have developed a novel nanodelivery vehicle called PolyDots that combines features of nanomicelles and polymer (poly[lactic-co-glycolic acid]) nanoparticles. We examined the in vivo and in vitro effects of PolyDot delivery both as empty particles and loaded with either fluorescent markers (Coumarin 6) or a therapeutic agent, vorinostat (HDAC inhibitor). Both glioma cells and GSC tumor spheres showed robust uptake of fluorescent PolyDots with initial endosomal uptake (punctate foci) followed by release from endosomes (diffuse fluorescence) indicating entry into both monolayer and 3-D tumor cell aggregates. Treatment of LN229 and U373 cells with fluorescent empty (PDC6) or vorinostat-loaded (PDV) PolyDots showed PDV-specific increase in acetyl-Histone 3 and acetyl-tubulin levels indicating uptake and biological effect of drug-loaded PolyDots in glioma cells. Lastly, fluorescent PolyDots injected into intracranial glioma xenografts in zebrafish showed discrete green punctae in the brain after 30 min and throughout the neuraxis at 24 hours, indicating neuraxis retention and wide-spread distribution of PolyDots in vivo after intracranial injection. Our results suggest that PolyDots can serve as a robust drug delivery platform that can encapsulate both drugs and imageable markers for nervous system delivery. Of particular relevance, they exhibit tunable release characteristics with potential for co-release of multiple agents with different release rates along with enhanced BBB penetration and diffusion in brain. PolyDots hence constitute a novel therapeutic delivery system that has the potential to overcome critical roadblocks in drug delivery for nervous system malignancies.
Prevention of Hyperuricemia by Allopurinol in Hypertensive Patients Treated with Chlorothiazide
Authors: James A. Nicotero, Eileen T. Scheib, Ramon Martinez, Gerald P. Rodnan, Alvin P. Shapiro
Journal: New England Journal of Medicine (1970)

A group of 18 patients with mild to moderate hypertension, receiving 1.0 gm of chlorothiazide per day, were treated with 300 mg of allopurinol daily for a two-week period. A change-over design was employed in which a two-week period on placebo was substituted for allopurinol. The level of serum urate rose from 4.8 to 5.7 mg per 100 ml with chlorothiazide and placebo; this value was reduced to 3.5 with addition of allopurinol and was accompanied by an approximate decrease of 25 per cent in uric acid excretion.
Abnormal CT Scans of the Brain in Asymptomatic Children with Acute Lymphocytic Leukemia after Prophylactic Treatment of the Central Nervous System with Radiation and Intrathecal Chemotherapy
Authors: Nili Peylan-Ramu, David G. Poplack, Philip A. Pizzo, Bruce T. Adornato, Giovanni Di Chiro
Journal: New England Journal of Medicine (1978)

Original Article from The New England Journal of Medicine — Abnormal CT Scans of the Brain in Asymptomatic Children with Acute Lymphocytic Leukemia after Prophylactic Treatment of the Central Nervous System with Radiation and Intrathecal Chemotherapy
Case 29-2003
Authors: Joshua D. Gutman, Camille N. Kotton, Alexander Kratz
Journal: New England Journal of Medicine (2003)

Presentation of CaseA 60-year-old man was evaluated in the clinic in mid-June because of fever, rigors, and sweats.The patient had been well until 10 days earlier, when anorexia, a sore throat, diffuse myalgia, arthralgia, headache, malaise, and fever (temperature, up to 39.3°C) developed, with frank rigors, night sweats, and dyspnea on climbing stairs. The sore throat had largely subsided by the time of the examination. There was no cough, nausea, vomiting, diarrhea, jaundice, light stools, dysuria, or rash. His only medication was atorvastatin, and he was allergic to sulfonamides.The temperature was 38.1°C. There was no rash, conjunctival . . .
Presence of Food Allergy Alters the Presentation of Pediatric Eosinophilic Esophagitis
Authors: Barry J. Pelz, Joshua B. Wechsler, Anusha Reddy Gaddam, Katie Amsden, Barry Wershil, Amir F. Kagalwalla, Paul Bryce
Journal: Journal of Allergy and Clinical Immunology (2016)
Effect of Reperfusion on Biventricular Function and Survival after Right Ventricular Infarction
Authors: Terry R. Bowers, William W. O'Neill, Cindy Grines, Mark C. Pica, Robert D. Safian, James A. Goldstein
Journal: New England Journal of Medicine (1998)

Although the salutary effects of reperfusion in patients with left ventricular infarction are well documented, the benefits in patients with acute right ventricular infarction are less clear.
A Study of the Value of Simple Protective Isolation in Patients with Granulocytopenia
Authors: William M. Nauseef, Dennis G. Maki
Journal: New England Journal of Medicine (1981)

To assess the value of simple protective isolation, we prospectively compared it with standard hospital care in 43 episodes of severe granulocytopenia, most occurring in patients with acute nonlymphocytic leukemia. Sterilized food and prophylactic oral antibiotics were not used. Twenty episodes in 17 patients were randomized to simple protective isolation (437 days), and 23 episodes in 20 patients to standard care (611 days). No statistically significant differences were observed in the overall incidence of infection, time to onset of first infection, or days with fever. Twenty-seven infections occurred in recipients of standard care (4.42 per 100 days), and 28 infections in isolated patients (6.41 per 100 days). Except for a threefold higher rate of bacteremia in patients in isolation (2.06 vs. 0.65 per 100 days), the profile of infection was similar in the two groups. Neither response to antileukemic therapy nor survival was improved by isolation. We conclude that protective isolation alone, as practiced in most hospitals, appears not to benefit granulocytopenic patients. (N Engl J Med. 1981; 304:448–53.)