tbuckley/cabot-search huggingface dataset:
AB0265 Prognostic Role of Serum Matrix Metalloproteinase-3 in Total Knee Arthroscopic Synovectomy
Authors: M. Lipina, M. Makarov, V. Amirdjanova, S. Makarov, A. Novikov, D. Goriachev, V. Pavlov
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Total arthroscopic knee synovectomy in rheumatoid arthritis helps for successful treatment of aggressive joint synovitis and reduces cartilage and bone destruction. Inopportune and inadequate indication for surgery can lead to synovitis reccurence. Is it possible to determine the risk factors of the synovitis recurrence using laboratory methods of diagnostics, namely serum matrix metalloproteinase-3 (MMP-3), which is one of the most popular prognostic marker in rheumatoid arthritis? <h3>Objectives</h3> To determine the prognostic role of MMP-3 in knee joint synovitis recurrence after total arthroscopic synovectomy (TAS). <h3>Methods</h3> The study includes 124 TAS for 108 rheumatoid arthritis patients. Serum MMP-3 was measured by ELISA before surgery and 3, 6, 12 months after. Prognostic role of MMP-3 was determined by receiver operating characteristic (ROC) curve analysis. The sensitivity, specificity, the area under the curve of ROC were calculated. <h3>Results</h3> Serum MMP-3 levels were elevated in all RA patients compared with healthy controls. The ROC analysis showed the area under the curve of 0,751, with the specificity of 69, 2% and the sensitivity of 73, 3%. MMP-3 also correlate with arthroscopic picture of cartilage degradation (Outerbridge classification) (r=0, 44) and morphologic characteristic, such as angiomatosis, of the synovial tissue. <h3>Conclusions</h3> High level of MMP-3 before surgery is a good predictor of the synovitis recurrence in RA. Degree of cartilage destruction and morphological characteristics of the synovial tissue are the adverse factors for the surgery results. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4344
Stem-Cell Treatment Brings a Historic Week in Gene Therapy
Authors: S. Jenks
Journal: JNCI Journal of the National Cancer Institute (1993)

Journal Article Stem-Cell Treatment Brings a Historic Week in Gene Therapy Get access Susan Jenks Susan Jenks Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 85, Issue 12, 16 June 1993, Pages 944–945, https://doi.org/10.1093/jnci/85.12.944 Published: 16 June 1993
Abstract 2674: Radio-sensitizer eluting nanoporous coatings on fiducials markers: Biological in-situ dose-painting for IGRT
Authors: Srinivas Sridhar, Dattatri Nagesha, Dayane Tada, Rajiv Kumar, G. Mike Makrigiorgos, Robert Cormack
Journal: Cancer Research (2011)

Abstract Image-guided radiation treatments routinely utilize implantable devices, such as radio-opaque fiducials or brachytherapy spacers, for improved spatial accuracy. We study the hypothesis that the therapeutic efficiency of IGRT can be further enhanced by biological in-situ dose painting (BIS-IGRT) using local delivery of radiosensitizers embedded within nanoparticles and nanoporous polymer matrices coating gold fiducial markers. Biocompatible polymers loaded with model molecules were coated as a thin film on gold fiducials. The nanoporous morphology of the polymer coatings allowed the controlled release of the molecules and nanoparticles. Two experimental approaches were studied: (i) a free drug release system Doxorubicin, a hydrophilic drug in Poly(methyl methacrylate (PMMA) coating and (ii) Poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles loaded with Coumarin-6, a model for a fluorescent hydrophobic drug, in a chitosan matrix applied as fiducial coating. Temporal release kinetics measurements in buffer were carried out using fluorescence spectroscopy. For flat gold films and gold fiducials coated with Doxorubicin in PMMA matrix, an initial release of Dox within the first few hours was followed by a sustained release over the course of next 3 months. Release of Dox from within PMMA matrix is dependent on the concentration of Dox, ratio of PMMA/Dox, thickness of PMMA/Dox coating on gold surface. The release profile of coumarin-6 loaded nanoparticles from chitosan film on gold fiducials showed a continuous release of NPs from the coating during forty days. (63±10)% of NPs were released in twenty days. After that, the release became slower and additional 37% of release was observed after twenty-days. Spatial release profiles in an agarose phantom were also measured and compared with release kinetics models. Results show that dosage and rate of release of these radiosensitizers can be precisely tailored to achieve the desired release profile for BIS-IGRT. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2674. doi:10.1158/1538-7445.AM2011-2674
360 EFFECTS OF NANOROD-CONJUGATED PEPTIDES FOR CELL TARGETING OF HEPATIC MACROPHAGES IN ACUTE AND CHRONIC MURINE LIVER INJURY
Authors: M. Bartneck, T. Ritz, H.A. Keul, M. Wambach, J. Bornemann, U. Gbureck, N. Gassler, J. Groll, C. Trautwein, F. Tacke
Journal: Journal of Hepatology (2012)
Abstract 993: Enhanced EGFR activity of prostate cancer cells by low pH, low glucose environment
Authors: Lakshmi R. Bollu, Zhang Weihua
Journal: Cancer Research (2011)

Abstract Background: Hypoxia, lack of nutrients and high aerobic glycolysis creates a microenvironment of high lactate, low glucose and low pH conditions within a solid tumor. The purpose of the current research is to study how the cancer cells adapt to a microenvironment with low pH, low glucose and high lactate levels with selective growth advantages. Methods: We simulated solid tumor microenvironment conditions by making DMEM media with low glucose (2mM), high lactate (10mM) and low pH (6.5). To study the growth advantages of this microenvironment, we determined the effect of low pH, low glucose and lactate medium on EGFR activation. Prostate cancer (PC3) cells were grown in six-well plates in 10% serum containing DMEM and serum starved overnight before they were changed to experimental medium. Control cells were grown in normal DMEM. We treated PC3 cells growing in normal DMEM and experimental medium with EGF at 1ng/ml. Protein samples were collected at different time intervals and subjected to western blotting to observe the activation of EGFR by using phospho EGFR antibody. EGFR and actin antibodies were used as control and loading controls, respectively. Results: Robust and sustained phosphorylation of EGFR for longer time was observed with EGF treatment when the cells were grown in low pH, low glucose and high lactate medium when compared with the cells grown in normal DMEM medium. Conclusion: The robust activation of EGFR with low pH, low glucose and high lactate medium suggest that the microenvironment containing high lactate provides growth or survival advantages to the cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 993. doi:10.1158/1538-7445.AM2011-993
Abstract 2100: Down regulation of HOXD10 by microRNA-10b enhances proliferation of glioblastoma multiforme derived cell lines
Authors: Arti B. Gaur, Mark A. Israel
Journal: Cancer Research (2010)

Abstract We sought to elucidate the role of a potentially oncogenic microRNA, microRNA-10b (mir-10b), in mediating the pathology of glioblastoma multiforme (GBM). MicroRNAs are small noncoding RNAs that play a critical role in developmental and physiological processes and are implicated in the pathogenesis of several human diseases including cancer. MicroRNAs function by regulating target gene expression post-transcriptionally. We have previously assessed the expression pattern of 241 mature human microRNAs in the NCI60 panel of cell lines and in a set of corresponding normal tissues (Gaur et al., 2007, Cancer Research. 67(6):2456-68). We found that microRNA expression patterns mark specific biological characteristics of tumors and/or mediate biological activities important for their pathobiology. In CNS tumor-derived cell lines, we identified mir-10b as a candidate oncogene, as it is expressed at higher levels in human GBM lines an primary GBM tissue than it is in normal brain tissue. Using several target prediction algorithms we identified a member of the homeobox family, Homeobox D10 (HOXD10), as a potential target of mir-10b. Our in vitro studies demonstrate that down regulation of mir-10b in GBM-derived cell lines results in increased expression levels of HOXD10. Additionally, our data indicate that down-regulation of mir-10b in human GBM lines leads to decreased proliferation and decreased anchorage independent colony formation in soft agar. Importantly, using a xenograft model in immune-deficient nude mice, we have observed human that GBM lines in which mir-10b expression is inhibited exhibit decreased tumor formation and growth in vivo. Furthermore, the decreased colony formation in soft agar observed as a result of mir-10b inhibition in GBM-derived cell lines is significantly antagonized by expression of siRNAs complementary to HOXD10 mRNA. Also, over expression of HOXD10 in GBM cell lines results in differentiation and decreased proliferation. Further experiments are ongoing to identify the specific role of HOXD10 in mediating the pathologic characteristics of GBM. An understanding of the pathways mediated by HOXD10 signaling may provide novel opportunities for therapeutic intervention. The National Brain Tumor Society (ABG) and the Theodora B. Betz Foundation (MAI) supported this work. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2100.
Presentation of hepatocellular antigens
Authors: Arash Grakoui, Ian Nicholas Crispe
Journal: Cellular and Molecular Immunology (2016)

The liver is an organ in which antigen-specific T-cell responses manifest a bias toward immune tolerance. This is clearly seen in the rejection of allogeneic liver transplants, and multiple other phenomena suggest that this effect is more general. These include tolerance toward antigens introduced via the portal vein, immune failure to several hepatotropic viruses, the lack of natural liver-stage immunity to malaria parasites, and the frequent metastasis of cancers to the liver. Here we review the mechanisms by which T cells engage with hepatocellular antigens, the context in which such encounters occur, and the mechanisms that act to suppress a full T-cell response. While many mechanisms play a role, we will argue that two important processes are the constraints on the cross-presentation of hepatocellular antigens, and the induction of negative feedback inhibition driven by interferons. The constant exposure of the liver to microbial products from the intestine may drive innate immunity, rendering the local environment unfavorable for specific T-cell responses through this mechanism. Nevertheless, tolerance toward hepatocellular antigens is not monolithic and under specific circumstances allows both effective immunity and immunopathology.
Side Glance: Flax Seed, Fruit, and Flower
Authors: J. Carter
Journal: JNCI Journal of the National Cancer Institute (1995)

Journal Article Side Glance : Flax Seed, Fruit, and Flower Get access Jack Carter, Ph.D. Jack Carter, Ph.D. Flax Institute at North Dakota State UniversityFargo Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 87, Issue 7, 5 April 1995, Page 484, https://doi.org/10.1093/jnci/87.7.484 Published: 05 April 1995
PREDICTION OF INTRAVENTRICULAR THROMBOSIS BY QUANTITATIVE IMAGING OF STASIS: A PILOT COLOR-DOPPLER STUDY IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION
Authors: Carolina Devesa Cordero, Lorenzo Rossini, Pablo Martinez-Legazpi, Candelas Perez Del Villar, Yolanda Benito, Alicia Barrio, Francisco Fernandez-Aviles, Raquel Yotti, Juan Carlos del Alamo, Javier Bermejo
Journal: Journal of the American College of Cardiology (2015)
Nasopharyngeal Tuberculosis Mimicking Nasopharyngeal Carcinoma on 18F-FDG PET/CT in a Young Patient
Authors: Yin Zhang, Yue Chen, Zhanwen Huang, Liang Cai, Jinbo Wu
Journal: Clinical Nuclear Medicine (2014)

A 30-year-old woman presented with a painless left upper neck swelling for more than a week. Fibrolaryngoscope examination suggested nasopharyngeal carcinoma. FDG PET/CT was performed for an initial staging, which revealed intense activity in the soft tissue mass in the nasopharynx and cervical lymph nodes. However, biopsy from the nasopharyngeal lesion demonstrated tuberculosis.
Abstract 5334: Tumor-associated-macrophage induces CD44 expression through microRNAs suppression promotes cancer progression and chemoresistance.
Authors: Takatsugu Ishimoto, Masayuki Watanabe, Nobuyuki Ozaki, Masaaki Iwatsuki, Yoshifumi Baba, Naoya Yoshida, Hideo Baba
Journal: Cancer Research (2013)

Abstract Objectives: CD44 has recently been identified as one of the cell surface markers associated with cancer stem cells (CSCs) in several types of tumor. We have reported the functional role of CD44 variant isoform in the maintenance of low reactive oxygen species (ROS) levels in gastrointestinal cancer cells. While, microRNA (miRNA) is involved in the pathogenesis of many cancers and the networks of miRNA regulate CSCs properties. However, the mechanism underlying the miRNA in the regulation of CSC marker is mostly unrevealed. Methods and Results: To investigate the mechanism in the regulation of CD44, we performed 384 miRNA quantitative RT-PCR array analysis in six human gastrointestinal cancer cell lines that differ in CD44 expression status. We found 59 miRNAs which are suppressed in CD44 high expressing cancer cells, and further investigated the predicted 33miRNAs which interact directly with CD44 3’UTR using the online miRNA target database. As a result in these analyses, we identified 2 miRNA candidates, which could regulate CD44 expression in gastrointestinal cancer. In this study, we especially focused on miR-328 and examined the functional relevance of miR-328 implicated in CD44 regulation. We found that gastrointestinal cancer cells transfected with miR-328 mimic decreased CD44 expression and led to inhibition of cancer cell growth and impairing chemo- and ROS- resistance. Furthermore, we investigated the mechanism underlying the regulation of miR-328 by using human macrophage and gastrointestinal cancer cell lines. We found that co-culture with tumor-associated-macrophages (TAMs) triggers the CD44 expression through suppression of miR-328 in gastrointestinal cancer cells and promote stem-like property that possess sphere-forming ability. Finally, we showed that TAMs are closely related with both CD44 over-expression and miR-328 under-expression in human gastric cancer tissue. Conclusions: These findings reveal that TAMs in tumor microenvironment contribute CD44 expression via miR-328 suppression, resulting in tumor progression through enhancing ROS defense. Citation Format: Takatsugu Ishimoto, Masayuki Watanabe, Nobuyuki Ozaki, Masaaki Iwatsuki, Yoshifumi Baba, Naoya Yoshida, Hideo Baba. Tumor-associated-macrophage induces CD44 expression through microRNAs suppression promotes cancer progression and chemoresistance. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5334. doi:10.1158/1538-7445.AM2013-5334
Essentials of Paediatric Cardiology
Authors: Mary Allen Engle
Journal: JAMA (1965)

This compact yet complete book can be recommended for those just being introduced to pediatric cardiology and those wishing to review a too distant acquaintance with the subject. It proceeds in logical fashion to describe the basic consideration of anatomy and physical diagnosis before summarizing the development of the heart and the transition from fetal to adult circulation. Abnormalities of cyanosis, heart murmurs, and cardiac failure are discussed before congenital and acquired heart disease. The developmental abnormalities are divided into the common and uncommon. The individual lesions are defined, and for each the altered physiology, clinical features, and management are summarized. In the section on tetralogy, this reviewer would have preferred other terms for two colloquialisms. The radiologic appearance of the lung fields is called "dry" rather than "oligemic" (or "excessively clear" or "radiolucent"), and the spells or attacks of paroxysmal dyspnea and cyanosis are repeatedly referred to as "turns."
MS-02 * TRANSCRIPTOME ANALYSIS IDENTIFIES THE PI3K/AKT/mTOR PATHWAY AS A TARGETABLE PATHWAY IN SCHWANNOMA
Authors: S. Agnihotri, K. Burell, S. Jalali, A. Alamsahebpour, N. Sabha, B. Krischek, K. Aldape
Journal: Neuro-Oncology (2014)

Schwannomas are common benign tumors of the vestibular nerve, or arise from nerves within the spinal canal. Although benign, both Spinal schwannoma (SS) and vestibular schwannoma (VS) cause significant morbidities. The current treatment strategies for VS and SS include surgery or radiation with each treatment option having associated complications and side effects. The transcriptional landscape of schwannoma remains largely unknown. We interrogated the transcriptome by gene-expression array analysis of 49 schwannomas and seven normal control vestibular nerves to identify tumor-specific gene expression patterns. We identified over 4000 differentially expressed genes between control and schwannoma with network analysis uncovering proliferation and anti-apoptotic pathways previously not implicated in VS. Using several distinct clustering technologies, we could not reproducibly identify VS subtypes or significant differences between sporadic and germline NF2 associated schwannomas suggesting that VS comprises of a highly similar entity. We next performed a transcript analysis comparing VS to SS. Surprisingly; we identified few differential transcripts demonstrating that schwannoma maybe a homogenous entity. Current studies are focused on DNA methylation profiling and genome wide sequencing analysis. To date our group and others have identified that inactivating mutations in NF2 is the most recurrent aberration in schwannoma. The most recurrent activated pathway in schwannoma was over-expression of PI3K/AKT/mTOR signaling pathway, which is directly druggable and we evaluated this pathway for therapeutic targeting. Testing compounds BEZ235 and PKI-587, both novel dual inhibitors of PI3K and mTOR, attenuated tumor growth in a cell line model of schwannoma. Our In vitro findings demonstrated that pharmacological inhibition of the PI3K/AKT/mTOR pathway with next generation compounds lead to decreased cell viability, and increased cell death. Future work is testing these compounds in vivo using relevant cell lines and primary cultures of schwannoma. Our findings implicate that targeting the PI3K/AKT/mTOR pathway may serve as a potential treatment strategy.
Abstract 4056: Fluorescence labeling of different live cell populations and subsequent analysis by flow and imaging cytometry
Authors: Leo L. Chan, Mark Alvarez, Dee Shen, Bo Lin, Wayne F. Patton
Journal: Cancer Research (2011)

Abstract Relative to conventional antibody-based immunohistological methods employing fixed and permeabilized cells, live cell imaging has presented challenges due to the lack of availability of compatible fluorescent probes. Additionally, instrument-dependent complications arise, such as the requirement that adherent cells be detached into a single cell suspension for flow cytometry, which can impact physiological response. A novel noncovalent fluorescence, long-term labeling technology was employed to stably incorporate green, red, or mixtures of green and red fluorescent dyes into the plasma membranes of different live cell populations. The dyes were well retained for up to 96 hours, faithfully transferred to daughter cells upon mitosis, yet displayed no tendency for nonspecific transfer to adjacent unlabeled cell populations. Since the described dyes do not covalently modify proteins within the cells, normal physiological responses are well preserved. Labeled cell populations were analyzed by conventional flow cytometry as well as with a novel imaging cytometer, the Cellometer Vision, incorporating inexpensive disposable counting chambers. Using a combination of bright-field and fluorescent images, the imaging system automates cell image acquisition and processing using novel counting algorithms for accurate and consistent measurement of cell concentration, fluorescent intensities and percentages of fluorescent positive cells. Results achieved using the imaging cytometer showed excellent correlation with those achieved by flow cytometry, but required 25-fold lower sample per analysis. As many as four cell subpopulations could be determined simultaneously. The described workflow provides a powerful, yet economic, approach applicable to long-term cell viability, cytotoxicity, cell adhesion, cell migration, lineage tracing and cell-cell fusion analysis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4056. doi:10.1158/1538-7445.AM2011-4056
THE IMPACT OF PRE-PROCEDURAL SERUM ALBUMIN LEVELS ON OUTCOME OF PATIENTS UNDERGOING TRANSCATHETER AORTIC VALVE REPLACEMENT
Authors: Edward Koifman, Marco Magalhaes Pereira, Itsik Ben-Dor, Sarkis Kiramijyan, Ricardo Escarcega Alarcon, Rebecca Torguson, Petros Okubagzi, Smita Negi, Nevin Baker, Sa’ar Minha, Paul Corso, Christian Shults, Lowell Satler, Augusto Pichard, Ron Waksman
Journal: Journal of the American College of Cardiology (2015)
Prevalence of <i>Helicobacter pylori</i> Infection and Histologic Gastritis in Asymptomatic Persons
Authors: Cornelius P. Dooley, Hartley Cohen, Patrick L. Fitzgibbons, Madeline Bauer, Maria D. Appleman, Guillermo I. Perez-Perez, Martin J. Blaser
Journal: New England Journal of Medicine (1989)

We estimated the prevalences of Helicobacter pylori (formerly called Campylobacter pylori) infection and histologic gastritis in 113 asymptomatic persons, using endoscopic biopsy of the gastric antrum and corpus. Unsuspected lesions, mainly mucosal erosions, were revealed at endoscopy in 16 subjects (14 percent). Gastritis was found in 42 subjects (37 percent), of whom 36 (32 percent of the total) were found to be infected with H. pylori on the basis of hematoxylin-eosin staining. H. pylori was not found in any of the 71 subjects with normal histologic features. Gastritis and H. pylori were noted in both the antrum and corpus in 75 percent of those infected (n = 27). The prevalence of H. pylori infection increased from 10 percent (2 of 20 subjects) in those between the ages of 18 and 29, to 47 percent (7 of 15) in those between the ages of 60 and 69, but the effect of age did not reach statistical significance. The prevalence of gastritis increased significantly with advancing age. Stepwise logistic regression analysis revealed that the relative risk for H. pylori infection associated with recent (within six months) antibiotic use was 5.8 (95 percent confidence interval, 1.5 to 22.1), whereas the relative risk was 6.5 (95 percent confidence interval, 1.4 to 29.2) for those who had never used bismuth compounds. We conclude that histologic gastritis and H. pylori infection commonly occur in the stomach of apparently normal persons and increase in prevalence with advancing age. All the subjects with H. pylori infection had gastritis, suggesting a possible etiologic role for the bacterium in the histologic lesion.