tbuckley/cabot-search huggingface dataset:
Case 21-2009
Authors: Eric G. Neilson, Alton B. Farris
Journal: New England Journal of Medicine (2009)

A 61-year-old woman was admitted to this hospital because of a 2-month history of abdominal pain and weight loss and an elevated serum creatinine level. On admission, physical examination was normal except for mild abdominal tenderness. Abdominal imaging revealed no specific abnormalities. Laboratory testing revealed anemia. The creatinine level initially fell after the administration of intravenous fluids but then rose and remained elevated. On the 8th day, erythema and pain developed in the left eye. A diagnostic procedure was performed.
OP0105 Accumulation of CD34+ hematopoietic stem cells in the initial inflammatory human fracture hematoma is driven by rantes and eotaxin
Authors: P. Hoff, T. Gaber, M. Hahne, C. Strehl, M. Fangradt, K. Schmidt-Bleek, G.R. Burmester, G. Schmidmaier, G.N. Duda, C. Perka, F. Buttgereit
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> We have previously shown the early phase of human fracture healing to be characterized by hypoxia which promotes inflammation and chemoattraction. Hypoxia is also known to promote proliferation, survival and migration of different stem/progenitor cells like mesenchymal stem cells, endothelial progenitor cells or hematopoietic stem cells (HSC). However, the clinical relevance of hypoxia and inflammation in the early phase of fracture healing for HSC remains unclear. <h3>Objectives</h3> To investigate immunological events in fracture healing, we quantified (i) CD34+ hematopoietic stem cells and (ii) inflammatory chemokines present in the early (&lt;72h) human fracture hematoma (FH) at the fracture gap. To investigate the chronologic development, we also analyzed hematomas which resulted from the transection of the femur in patients receiving a total hip arthroplasty (THA). The THA-hematomas (THA-H) were defined as a model for fracture hematomas at time point 0h. <h3>Methods</h3> The proportion of HSC in the fracture hematoma from healthy patients (n=42) and patients receiving a THA (n=20) was analyzed by flow cytometry. Secreted factors were quantified by multiplex suspension array. <h3>Results</h3> A fracture destroys bone architecture and vascular network leading to bioenergetically restricted conditions such as hypoxia within the fracture hematoma. Although the cells present have to face those conditions, we were able to find a higher proportion of CD34+ hematopoietic stem cells in the FH as compared to THA-H (7.6±1.5 vs. 3.8±0.5% of mononuclear cells) indicating proliferation and/or immigration of HSC in the FH. As CD34+ hematopoietic stem cells express CCR3, we investigated the concentrations of its ligands RANTES and Eotaxin. Indeed, both chemokines were present at significantly higher concentrations in the FH as compared to THA-H (RANTES: 16867±1632 vs. 9830±1397 pg/ml, p&lt;0.01; Eotaxin: 327±76 vs. 125±15 pg/ml, p&lt;0.001). We also identified the macrophage migration inhibitory factor (MIF) to be significantly increased in the FH (179431±28538 vs. 21751±2973 pg/ml, p&lt;0.001). <h3>Conclusions</h3> Hypoxia and other bioenergetically adverse conditions in a FH contribute to the induction of inflammation, including the secretion of RANTES, Eotaxin and MIF. We suppose the high concentrations of RANTES and Eotaxin to facilitate the immigration of CD34+ HSC. The initial hypoxic conditions also mediate the secretion of the proinflammatory MIF which has been already shown to be important for successful fracture healing. Thus, the inflammatory microenvironment in the FH is among the crucial factors determining fracture healing. <h3>Disclosure of Interest</h3> None Declared
TCT-311 Pressure Wire Pullback Using the Instantaneous Wave-Free Ratio (iFR) Can Identify Stenoses and Predict the Improvement After Stenting
Authors: Sukhjinder S. Nijjer, Sayan Sen, Ricardo Petraco, Javier Escaned, Mauro Echavarria-Pinto, Rasha Al-Lamee, Nicolas Foin, Iqbal S. Malik, Ghada Mikhail, Amarjit Sethi, Masood A. Khan, Christopher S. Baker, Michael Bellamy, Darrel P. Francis, Carlo Di Mario, Justin E. Davies
Journal: Journal of the American College of Cardiology (2014)
P701Systemic inflammation markers and vascular damage in rheumatoid arthritis
Authors: AR Babaeva, KS Solodenkova, EV Cherevkova, MN Usachiova
Journal: Cardiovascular Research (2014)

Purpose: inflammation contributes to the pathogenesis of vascular damage in cardiovascular disease (CVD). Rheumatoid arthritis (RA) is associated with systemic inflammation and is considered as independent risk factor of cardiovascular events. The severity of RA can be assessed by disease activity score (DAS 28) and is correlated with the levels of most significant pro-inflammatory cytokines, such as TNF-alpha, IL-1beta, IL-6. The aims of this study were to determine the relationship between inflammatory markers (TNF-alpha, IL-1, IL-6) and the incidence of CVD clinical manifestation in RA pts. Methods: 120 pts with determined RA were monitored during 12 months. We evaluated activity of RA by DAS 28 index, serum C-reactive protein and pro-inflammatory cytokines (TNF-alpha, IL-1-beta, IL-6). At the same time as we assessed the systemic cardiovascular risk and incidence of cardiovascular events: severe hypertensive crisis, arrhythmia, unstable angina, myocardial infarction, stroke, sudden cardiac death. Results: In observed group the most common cardiovascular manifestations were: arterial hypertension, arrhythmias, angina pectoris, chronic heart failure. The cases of complicated hypertensive crisis, arrhythmias, unstable angina and myocardial infarction were preferably revealed in pts with high activity RA (DAS 28 >5). There was a significant correlation between the levels of TNF-alpha, IL-1beta, IL-6 and cardiovascular risk in RA pts. According the χ2 criterion the incidence of cardiovascular events was significantly more in pts with elevated cytokine level. Conclusions: The increased serum levels of TNF-alpha, IL-1beta, IL-6 in RA may play a significant role in CVD manifestation and can be considered as predictors of cardiovascular events.
262 STUDY ON CD3+CD8+ HLA-A2 T CELLS WITH SPECIFICITY TO THE DIFFERENT HEPATITIS B VIRAL PEPTIDES IN THE PATIENT WITH HEPATITIS B ASSOCIATED HEPATOCELLULAR CARCINOMA
Authors: J.-L. Cheng, L.-W. Wang, B. Lv, C.-L. Qiu, Y.-C. Ai, Y. Zhang
Journal: Journal of Hepatology (2012)
Abstract 2630: DNA repair single nucleotide polymorphisms interact with indoor tanning and melanoma
Authors: Li Luo, Jenna M. Lilyquist, Chris Stidley, Kristina Flores, Kirsten White, Susan Paine, Melissa Gonazales, Esther Erdei, Rachel Vogel, DeAnn Lazovich, Marianne Berwick
Journal: Cancer Research (2012)

Abstract Development of melanoma has previously been associated with compromised DNA repair which can be analyzed by genotyping SNPs in DNA repair genes. The Minnesota Skin Health Study collected DNA samples to investigate the hypothesis that DNA repair SNPs are associated with increased risk for melanoma among tanning bed users. 92 SNPs, with minor allele frequencies greater than 0.05, were analyzed in 20 genes for 1659 samples_893 melanoma cases and 766 controls. The SNPs were genotyped on the Illumina BeadExpress GoldenGate platform. The SNPs were then analyzed for individual SNP associations with melanoma, haplotype associations with melanoma, individual SNP interactions with ever/never indoor tanning, and haplotype interactions with ever/never indoor tanning after adjustments for age, gender, and phenotypic index. There were several SNPs that had an association with melanoma including: six SNPs in three nucleotide excision repair genes ERCC4, ERCC6 (rs4253190, adjusted OR=1.19, 95%CI: 1.04-1.37; rs4838518, adjusted OR=0.86, 95%CI: 0.75-0.99; rs4253121, adjusted OR=0.84, 95%CI:0.71-0.99), and XPC, one SNP in base excision repair gene RFC1, one SNP in direct reversal DNA repair gene MGMT, and one SNP in a novel gene FBRSL1(rs4883571, adjusted OR=0.87, 95%CI: 0.75-1.00). There were four haplotype associations with melanoma including one ERCC6 block (rs10745261, rs6537537, rs4253121: GGA, adjusted OR: 0.84, 95%CI: 0.71-0.99), two MGMT blocks, and one XPC block. Additionally, there was one SNP in FBRSL1 (rs4883557, p=0.0056) and one SNP in ERCC6 (rs10745261, p=0.025) that had an interaction with indoor tanning along with two haplotypes including one block in ERCC6 (rs10745261, rs6537537, rs4253121: AAG, p=0.0355) and one block in MGMT. While the interaction analyses were suggestive, the study is limited because none of the SNPs or haplotypes were only significant (p&amp;lt;0.05) before Bonferroni correction. Further analyses of the function of these SNPs will be necessary, particularly for ERCC6, which had SNPs that were significant in every analysis, and FBRSL1, which is a novel gene whose function is unknown. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2630. doi:1538-7445.AM2012-2630
Impact of Inhaled Nitric Oxide on Platelet Aggregation and Fibrinolysis in Rats with Endotoxic Lung Injury
Authors: NATHALIE KERMARREC, PATRICIA ZUNIC, SADEK BELOUCIF, JOËLLE BENESSIANO, LUDOVIC DROUET, DIDIER PAYEN
Journal: American Journal of Respiratory and Critical Care Medicine (1998)

As inhaled nitric oxide (iNO) may differently increase bleeding time (BT) and inhibit platelet aggregation in normal and lung-injured patients or experimental models, we studied the effects of iNO on hemostasis in presence and absence of an endotoxic lung injury in the rat. Eight hours after intratracheal administration of endotoxin (lipopolysaccharide [LPS]) or its solvent (phosphate-buffered solution [PBS]), four groups of rats were randomized according to the presence or absence of 15 ppm iNO added for an additional 10 h. We measured BT, ex vivo platelet aggregation, plasma fibrinogen, euglobulin clot lysis time (ECLT), and platelet and aortic cyclic guanosine 5'-monophosphate (cGMP) contents. Acute lung inflammation did not influence BT, but increased platelet aggregability, fibrinogen levels, and platelet and aortic cGMP. In control and endotoxic rats, iNO increased BT, reduced platelet aggregability, and increased platelet cGMP. iNO increased aortic cGMP only in healthy rats. ECLT was increased by LPS and unchanged with iNO. These results suggest that the extrapulmonary "systemic" effects induced by iNO on hemostasis were not strictly similar in healthy and LPS rats, inflammation inducing proper changes in coagulation parameters. However, iNO attenuated the procoagulant activity induced by acute lung inflammation, suggesting a potentially beneficial effect of this therapy.
OP0172 Characterising Arthralgia in the Preclinical Phase of Rheumatoid Arthritis Using Magnetic Resonance Imaging
Authors: H.W. Van Steenbergen, J.A. van Nies, T.W. Huizinga, J.L. Bloem, M. Reijnierse, A.H. van der Helm-van Mil
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> The phase of arthralgia is the earliest moment to clinically recognize patients who will develop Rheumatoid Arthritis (RA). Previous imaging studies in the phase of arthralgia without clinical arthritis showed that subclinical inflammation precedes RA development. It is unknown which symptoms and characteristics relate to subclinical joint inflammation as measured by MR-imaging. Among all arthralgia patients, those with Clinically Suspect-type Arthralgia (CSA) are suspect to progress to arthritis according to the clinical judgment of their rheumatologists. <h3>Objectives</h3> We determined the symptoms and characteristics of patients with Clinically Suspect Arthralgia that had inflammation on MRI. <h3>Methods</h3> 102 patients with CSA and without clinical arthritis were included. At baseline, they filled questionnaires, underwent joint counts and unilateral 1.5T MRI of MCP2-4, wrist and MTP1-5 joints. Synovitis, bone marrow edema (BME) and tenosynovitis were scored according to the RAMRIS method. Symptoms and signs were related to MRI-inflammation (based on MRI-scores in symptom free controls, a sum of the scores for synovitis, BME and tenosynovitis ≥3 was considered as positive for MRI-inflammation). Whether certain clinical characteristics and MRI-inflammation frequently occurred together was studied by Partial Least Squares regression. All CSA patients were followed longitudinally. <h3>Results</h3> 44% of the CSA patients had subclinical MRI-inflammation. Synovitis was the most prevalent inflammatory feature on MRI (20%). Patients with MRI-inflammation were older and more frequent anti-citrullinated peptide antibodies (ACPA)-positive than patients without MRI-inflammation (p&lt;0.001 and 0.049 respectively). A combination of symptoms and characteristics (including subacute symptom onset, initial localization in large joints, or in lower extremities, morning stiffness ≥60 minutes, elevated acute phase reactants and presence of autoantibodies) explained 42% of the variance in MRI-inflammation. Hence, no specific combination of characteristics was found to be discriminative for the presence of subclinical inflammation. Despite a still limited follow-up duration, 35% of the patients with MRI-detected subclinical inflammation that were followed for at least four months developed clinical arthritis or RA. <h3>Conclusions</h3> Subclinical inflammation as measured by MRI is present in 44% of CSA patients. A combination of symptoms/characteristics incompletely differentiated patients with and without MRI-inflammation. Follow-up will reveal which characteristics relate to RA development. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2092
Glucagon Treatment of Insulin Reactions
Authors: Harold Elrick, Thomas A. Witten, Yoshiaki Arai
Journal: New England Journal of Medicine (1958)

PREVIOUS studies have shown that glucagon is capable of counteracting the hypoglycemic effect of insulin in normal man,1 in psychotic patients undergoing insulin-coma therapy2 and in diabetic patients.3 In this paper evidence is presented that glucagon is an effective agent for the treatment of insulin reactions in diabetic patients.Experimental DataPatientsEighteen hospitalized male diabetic patients, thirty to sixty-nine years of age, were studied. The known duration of the diabetes in the group ranged from one to twenty-five years. All patients required insulin; the daily requirement ranged from 12 to 150 units. All had a history of previous insulin . . .
FRI0104 Cohort study of infectious disease risk management in RA patients receiving tocilizumab at 48 weeks (ACT4U-study48)
Authors: A. Ihata, H. Hagiyama, S. Nagaoka, J. Obata, K. Miyachi, H. Yamada, S. Hirohata, N. Koido, M. Yamasaki, K. Miyagi, S. Ohno, D. Kishimoto, K. Takase, M. Hama, R. Yoshimi, A. Ueda, M. Takeno, Y. Ishigatsubo
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> An administration of tocilizumab (TOC) rapidly reduced inflammatory markers such as CRP and ESR, which made the diagnosis of infection difficult. According to PMS for 28 weeks, 553 events of infection and 163 events of serious infection (SI) were reported. There is a real need for the reduction of the infection risk. <h3>Objectives</h3> To validate the deterrence effect of infectious disease risk management (IDRM) in RA patients with TOC. <h3>Methods</h3> Forty-nine RA patients with TOC were enrolled from 4 universities and affiliated clinics. They were required to comply with IDRM policy during observation period. Primary endpoint was an occurrence frequency of SI (OFSI). <h3>Results</h3> Although 17 events of infection and 3 events of SI were observed, neither pneumonia nor cellulitis occurred. Risk factors were 10 year over disease duration and 5mg over CS dosage at the baseline. CDAI was changed from 61.1±3.7 to 18.3±4.4. The dose of CS was reduced by 41.8%. Persistence rate of TOC was 69.4% at 48 weeks. <h3>Conclusions</h3> In spite of higher frequency of concomitant lung disease, the incidence of respiratory infection and OFSI were lower in our study than PMS. IDRM could contribute to reduction of OFSI especially in respiratory infection. <h3>References</h3> Koike T, et al. Ann Rheum Dis 2011:70:2148-2151 <h3>Disclosure of Interest</h3> None Declared
Occlusion of Large Cerebral Vessels in Sickle-Cell Anemia
Authors: James A. Stockman, Michael A. Nigro, Mark M. Mishkin, Frank A. Oski
Journal: New England Journal of Medicine (1972)

Seven patients with sickle-cell anemia and neurologic deficits secondary to Central-nervous-system dysfunction were studied with cerebral angiography after careful preparation. Preparation of the patient reduced the level of S hemoglobin to less than 20 per cent before study, and all studies were performed without sequelae. Six of the seven patients were found to have partial or complete occlusion of large cerebral vessels. The internal carotid artery was involved in all six patients. Disease in the anterior and middle cerebral arteries as well as the vertebral artery was also observed. Two patients had repeat studies. The disease appeared to be progressive. These findings, rarely reported, indicate that the common clinical assumption that the Central-nervous-system manifestations of sickle-cell anemia are a consequence of exclusively small-cerebral-vessel obstruction may in fact be erroneous.
OP0220 Innovative anti-inflammatory strategy in arthritis using PBEF sirna-mediated silencing in LY-6CHIGH monocytes
Authors: J. Presumey, G. Courties, L.-M. Charbonnier, V. Escriou, D. Scherman, Y.-M. Pers, P. Louis-Plence, H. Yssel, J. Pène, D. Kyburz, S. Gay, C. Jorgensen, F. Apparailly
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The inflammatory Ly-6C<sup>high</sup> monocyte subset is essential in innate immunity but it also contributes to the pathogenesis of many inflammatory and autoimmune disorders. Strategies to manipulate this cell subset are thus of therapeutic importance. The pre-B-cell colony enhancing factor (PBEF/visfatin/Nampt) is an essential enzyme in the NAD biosynthetic pathway that also exerts a key role in the persistence of inflammation through the induction of the expression of the TNF-α and IL-6 pro-inflammatory cytokines. PBEF is highly expressed in the serum of patients with a variety of inflammatory disorders, including rheumatoid arthritis. <h3>Objectives</h3> The present study aimed at evaluating the therapeutic potential of delivering anti-inflammatory siRNA to Ly6C<sup>high</sup> monocytes for immuno-intervention in arthritis. <h3>Methods</h3> Using the collagen-induced arthritis mouse model, we show that the delivery of a short interfering (si)RNA against PBEF into the Ly-6C<sup>high</sup> monocytes is able to reduce molecular and cellular markers of inflammation, both systemically and locally, thereby inhibiting disease progression. Upon intravenous injection, siRNA localize to circulating Ly-6C<sup>high</sup> monocytes, but not to Ly-6C<sup>low</sup> monocytes. Efficient silencing of PBEF in Ly-6C<sup>high</sup> monocytes reduces the downstream TNF-α and IL-6 production and lead to decreased number of infiltrating leukocytes in arthritic joints and to reduced number of splenic pathogenic T cells. Importantly, when the siRNA-containing liposome formulation is tailored for translation to the clinic and tested on human peripheral blood mononuclear cells, inhibition of PBEF and its downstream mediators in CD14<sup>+</sup>CD16<sup>-</sup> monocytes reduces the generation of Th17 cells. <h3>Conclusions</h3> Overall, our preclinical results demonstrate the feasibility and utility of targeting disease-causing genes with RNAi technology in Ly-6C<sup>high</sup> monocytes for therapeutic intervention in arthritis. <h3>Disclosure of Interest</h3> None Declared
Metastases of Germ Cell Tumor Mimicking Paget's Disease
Authors: Archi Agrawal, Sushma Awasare, Ramesh Agrawal, Ramesh Asopa
Journal: Clinical Nuclear Medicine (2010)

A bone scan done for a 58-year-old man with intense pain in the lower back and pelvis, showed intense MDP uptake in most of the pelvis, in the proximal aspect of the left femur, D-8 vertebra, and a focal area of uptake in the skull. The features were suggestive of Paget's disease. An x-ray of pelvis revealed multiple sclerotic lesions in the pelvis (left ischium, acetabulum, and supra-acetabular regions of left ilium, right acetabulum, the left femoral neck, and left hip joint), suggesting metastases. On clinical examination, he had a left hydrocele. Peroperatively, a mass was found in the left testis for which a left orchidectomy was done. The histopathology was high-grade malignant germ cell tumor. Fine needle aspiration cytology from the pelvis confirmed the presence of metastases from germ cell tumor.
AB0903 Sleep quality and associated factors in ankylosing spondylitis: Relation with disease parameters, psychological status and quality of life
Authors: I. Batmaz, M.A. Sariyildiz, B. Dilek, Y. Bez, M. Karakoç, R. Cevik
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Disturbance in sleep quality is important problem in ankylosing spondylitis(AS). Sleep problems may be affected from different aspects. <h3>Objectives</h3> We aimed to investigate sleep quality and its association with the disease specific parameters, psychological status and quality of life in patients with AS. <h3>Methods</h3> 80 AS patients (male/Female: 60/20) fullfilling modified New York criteria and 52 controls (M/F: 33/19), age and gender matched, were included into the study. Sleep quality was assessed using Pittsburg Sleep Quality Index (PSQI) in patients and controls. Pain was measured with visual analogue scale (VAS). Disease activity and functional status assesed by Bath AS Disease Activity Index (BASDAI) and Bath AS Functional Index (BASFI). Bath AS Metrology Index (BASMI) is used to evaluate mobility restrictions. Bath AS Radiology Index (BASRI) was used for evaluating the radiological damage. Psychological status and quality of life were assessed with the Hospital Anxiety-Depression Scale (HADS) and AS quality of life (ASQoL) measurements. <h3>Results</h3> The patients with AS had significantly higher scores in subjective sleep quality, habitual sleep efficiency domains (p&lt;0.001) and the total sore of the PSQI (p&lt;0.05) compared to the healthy controls. Poor sleep quality (total PSQI score) was positively correlated with increased pain, poor quality of life, higher depressed mood, more active disease and restrictive mobility (p&lt;0.01, p&lt;0.01, p&lt;0.01, p&lt;0.01 and p&lt;0.05, respectively). Also Pain was an independent contributor of poorer sleep quality (p=0.002). <h3>Conclusions</h3> Sleep quality disturbed in AS. Pain, disease activity, depression, quality of life and increased mobility restriction have important impact on sleep quality. <h3>Disclosure of Interest</h3> None Declared