tbuckley/cabot-search huggingface dataset:
SAT0553 Incident Non-Infectious Uveitis in Spain: Yearly Cost of Resources
Authors: R. Blanco, E. Pato, A. Sellas, A. Adán, J. M. Benitez del Castillo, A. Bañares, A. Ruiz Zorrilla, M. Costi
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Non infectious uveitis (NIU) is frequently associated to systemic auto-immune diseases, ranging from 2% (inflammatory intestinal disease) to 40% (ankylosing spondylitis), while up to 50% of NIU are idiopathic. There is limited data on NIU burden of disease and its associated costs in Spain. <h3>Objectives</h3> The purpose of this study was to quantify the yearly costs associated to the management of NIU in Spain. <h3>Methods</h3> A systematic literature search tried to find epidemiological data and direct costs of NIU management in Spain. Information gaps were secondarily identified and filled using expert panel consensus: questionnaire consensus development on resource use items to be responded; experts' individualized response collection; and experts' consensus agreement on relevant resources whose costs should be calculated. Five experts, two ophthalmologists and three rheumatologists from multi-disciplinary uveitis units at tertiary hospitals, participated. All cost calculations were based on 2011 Spanish National Health System rates for procedures and drug approved prices <h3>Results</h3> From the literature search it was found that there were 9398 incident NIU cases in 2011 in Spain of which 55% were female, and 70% aged between 16 and 65 years. Acute Anterior Uveitis (AAU) showed the highest incidence (55%) followed by Posterior Uveitis (PU) and Panuveitis (PnU) (15% each), and Adult Chronic Anterior Uveitis (ACAU), Pediatric Chronic Anterior Uveitis (PCAU) and Intermediate Uveitis (IU) (5% each). Table 1 shows total associated costs per type of resource used, according to the type of uveitis. Initial drug treatment was associated to the highest cost and included topical cyclopegics, midriatics and steroids, systemic steroids, sulfasalazine, antimetabolites, T-cell inhibitors and TNF-alpha inhibitors. The second highest cost resource was surgical procedures, including cataract, glaucoma treatment, iridotomy and vitrectomy. It also shows total associated costs per patient and per type of NIU. PnU, PU, and AAU management were associated to the highest associated costs. <h3>Conclusions</h3> Clinical management of incident NIU in Spain is associated to high yearly costs of resources, mainly originated from initial drug treatment, surgical treatment of complications and diagnostic visits. Prevention of NIU could potentially originate savings to the National Health System <h3>Disclosure of Interest</h3> None Declared
Abstract SY38-04: Altered metabolic enzymes and metabolites in cancer
Authors: Yue Xiong
Journal: Cancer Research (2012)

Abstract Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599, USA. Molecular and Cell Biology Lab, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China Altered metabolic regulation was recognized as the first tumor phenotype and broadly used in the clinic for tumor detection. Recent findings of frequent mutations targeting gene encoding metabolic enzymes in cancer have renewed interest in cancer metabolism. Eight metabolic genes encoding for four metabolic enzymes, IDH1, IDH2, SDH and FH, have been found to be mutated in a wide range of human cancers. Our recent findings revealed that associated with the mutations of these four enzymes are the accumulation of three metabolites, 2-hydroxyglutarate (2-HG), succinate and fumarate, that act as antagonists of α-ketoglutarate (α-KG) and negatively regulate the activity of multiple α-KG-dependent dioxygenases, including both histone demethylases and newly discovered TET family of DNA hydroxylases. Consistent with these in vitro and cell culture studies, tumor with IDH1/2 mutation exhibit altered methylation on both histone and DNA. These findings suggest not only roles of these putative oncometabolites in epigenetic control and gene regulation, but also potential new targets for cancer therapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr SY38-04. doi:1538-7445.AM2012-SY38-04
Adalimumab Pricing and Market Exclusivity for Biologics
Authors: Aaron S. Kesselheim
Journal: New England Journal of Medicine (2010)

To the Editor: In our Perspective article about market exclusivity for biologics (Nov. 12, 2009, issue),1 the table provided the cost to consumers for adalimumab for the treatment of Crohn's disease, for which the approved dose is an induction of 160 mg, followed by 80 mg 2 weeks later, and then 40 mg every other week for a year. The value of $50,933 was derived from a publicly available listing in 2009 on Drugstore.com, a widely used Internet pharmacy site, where the product was described as “1 box = two 20 mg/0.4 mL syringes” and the price per box was . . .
Synergistic Differentiation-Promoting Activity of Interferon and Tumor Necrosis Factor- : Role of Receptor Regulation on Human Neuroblasts
Authors: P. G. Montaldo, R. Carbone, M. V. Corrias, P. C. Ferraris, M. Ponzoni
Journal: JNCI Journal of the National Cancer Institute (1994)

Background : Interferon γ (IFN-γ) and tumor necrosis factor-α (TNF) synergize in inducing human neuroblastoma cells to differentiate terminally in vitro into mature nonproliferating neurons. The mechanisms by which this synergistic activity takes place are still obscure. Purpose : To understand the basis of IFN-γ-TNF synergism, we investigated the constitutive equipment of receptors to IFN-γ and TNF in two human neuroblastoma cell lines (i.e., LAN-5 and GI-LI-N) and their quantitative and functional variations following treatment with IFN-γ or TNF. Methods : IFN-γ receptors and TNF receptors were assessed and functionally characterized by radioreceptor-binding assay before and after treatment of the cells with IFN-γ or TNF. The TNF receptor subtypes were identified by the reverse transcriptase-polymerase chain reaction, chemical cross-linking of receptors to iodinated TNF, and inhibition of TNF binding by type-specific anti-TNF receptor monoclonal antibodies. The effects of cytokines on cell differentiation were assessed by thymidine incorporation inhibition and morphologic maturation. Results : No quantitative or functional modification of IFN-γ receptors was observed in TNF-treated cells. However, after treatment with IFN-γ, TNF receptor numbers were enhanced to a different extent in both cell lines. The two neuroblastoma cell lines expressed, both constitutively and after IFN-γ induction, only one species of TNF receptor, i.e., the p80 form in LAN-5 and the p60 form in GI-LI-N. Sequential treatment with IFN-γ followed by TNF, but not in the opposite order, could reproduce the early effects of differentiation in neuroblastoma cells, supporting a role for TNF receptor up-regulation as a basis for the cooperation between the two cytokines. Conclusion : The results strongly suggest that receptor regulation can be at least one mechanism by which IFN-γ and TNF exert their synergistic effects. Moreover, it appears that the two TNF receptor types are redundant in signaling neuroblastoma cell differentiation. Implications : Our findings can provide a guideline for a rational design of experimental differentiation-based therapeutic protocols in patients with neuroblastoma. [J Natl Cancer Inst 86:1694–1701, 1994]
Adverse Cardiovascular and Central Nervous System Events Associated with Dietary Supplements Containing Ephedra Alkaloids
Authors: C A Haller, N L Benowitz
Journal: New England Journal of Medicine (2000)

Dietary supplements that contain ephedra alkaloids (sometimes called ma huang) are widely promoted and used in the United States as a means of losing weight and increasing energy. In the light of recently reported adverse events related to use of these products, the Food and Drug Administration (FDA) has proposed limits on the dose and duration of use of such supplements. The FDA requested an independent review of reports of adverse events related to the use of supplements that contained ephedra alkaloids to assess causation and to estimate the level of risk the use of these supplements poses to consumers.
P964 MICROWAVE ABLATION OF LIVER METASTASES FROM COLORECTAL CANCER USING A NEW DEVICE: PRELIMINARY RESULTS
Authors: G. Poggi, L. Solbiati, F. Meloni, B. Montagna, M. Bezzi, A. Campatelli, L.G. Carata, A. Filippone, D. Gasperini, C. Gazzera, F. Grasso, C.L. Mezzolla, A. Noto, G. Riva, S. Sartori, F. Stacul, G. Vallone
Journal: Journal of Hepatology (2014)
Duodenal duplication cyst causing acute recurrent pancreatitis, managed curatively by endoscopic marsupialization
Authors: V. Arantes, S. Nery, S. Starling, W. Albuquerque, L. Alberti
Journal: Endoscopy (2012)

Duodenal duplication cyst (DDC) is a rare congenital anomaly that typically presents with symptoms during childhood [1]. Acute pancreatitis and biliary obstruction have rarely been reported in adults [2] [3]. Two modalities of treatment have been described for DDCs: surgical [4] [5] and endoscopic, by means of cyst marsupialization [1]. The rationale behind cyst marsupialization is to establish communication between the cyst cavity and the duodenal lumen so that the cystic contents can drain continuously into the duodenum [1].
OC-031 Development of a collaborative inpatient alcohol strategy
Authors: M York-Smith, R Thompson, G Burrell, S Green, K Phekoo, J Fehler, V P B Grover
Journal: Gut (2012)

<h3>Introduction</h3> Alcohol-related medical admissions rise by 10% every year at The Hillingdon Hospitals NHS Foundation Trust, frequently readmitting patients to the medical wards. We sought to work collaboratively, crossing traditional boundaries between different care providers, to improve the quality of care provided to patients admitted with alcohol-related problems. <h3>Methods</h3> Acute and community care providers worked with the Collaboration for Leadership in Applied Health Research and Care (CLAHRC) for Northwest London to identify and overcome barriers to care and communication for patients with alcohol-related admissions to the medical wards at a District General Hospital. We employed a Psychologist with appropriate addiction and alcohol expertise as an Alcohol Specialist Practitioner (ASP). The role of the ASP was to: (1) educate, empower and support staff to better identify patients with alcohol problems deliver; (2) perform a comprehensive psychological assessment; (3) deliver psychological interventions; (4) encourage patient engagement with community alcohol services when assessed as appropriate; (5) measure outcomes as a result of service implementation. 103 medical inpatients (76 male; 27 female) were referred to the new inpatient service between 1 August 2011 and 31 December 2011. We compared the rate of engagement with alcohol services of the cohort referred to the ASP, to a cohort of 41 patients admitted to the Gastroenterology ward in 2010 with an alcohol-related admission, formally referred to alcohol services on discharge, by the Ward Manager. We studied the 30-day readmission rates between patients who did and did not engage with psychological interventions/community services. The Student9s t-test was used for statistical analysis between groups. <h3>Results</h3> 7 of the 103 patients referred to the service subsequently died as an inpatient, a further seven were abstinent at the time of referral. Analysis was performed on data from the remaining 89 patients. 69.5% of patients referred to the inpatient service engaged with the ASP and/or community alcohol services. This compares to an engagement rate of 15% with community alcohol services before the appointment of the ASP, (p 63% of those that declined the assessment/intervention of the ASP were readmitted within 30 days, significantly more than intervention group (p <h3>Conclusion</h3> Employment of a Psychologist as an ASP on the medical wards significantly improved the subsequent engagement of patients with community alcohol services. Patients engaged with community alcohol services had a significantly lower rate of alcohol-related readmissions, compared to those who did not engage. <h3>Competing interests</h3> None declared.
Abstract 4038: Identification of microRNAs involved in colorectal cancer progression
Authors: Manuela Gariboldi, Andrea Lampis, Viktorija Sokolova, Sara Pizzamiglio, James F. Reid, Susanna Zanutto, Claudia Bertan, Federica Perrone, Paolo Verderio, Ermanno Leo, Marco Pierotti
Journal: Cancer Research (2010)

Abstract MicroRNAs (miRNAs) are negative regulators of gene expression during various basic biological processes such as development, cellular differentiation, proliferation and apoptosis. Altered expression of miRNAs is associated with development and progression of various human cancers, where they mainly regulate the translation of oncogenes and of tumor suppressor genes. In colorectal cancer (CRC), these regulators complement the Vogelstein multi-step model of pathogenesis and have the potential of becoming a novel class of tumor biomarkers and of therapeutic targets. To identify miRNAs deregulated in CRC that could be used for diagnosis and prognosis, we examined by Quantitative Real-Time PCR the global expression of 378 mature miRNAs in 40 CRCs and their paired non-tumor tissues. Non-parametric confidence intervals for each miRNA where calculated and twenty-four miRNAs whose expression is significantly altered in tumors respect to the normal tissue where identified (Bonferroni P&amp;lt;0.05). Part of them, such as miR-21, miR-31 or miR-183 has been previously reported in CRC. MiR-135b, with the highest expression in the tumors is a promising tumor related miRNA. We subsequently evaluated the association of the 24 miRNAs with different clinical characteristics of the samples such as tumor site, disease status, TNM staging, expression of CEA and CA199, and presence of alterations in the molecular markers of CRC progression (APC, TP53, KRAS and loss of 18q arm). MiRNAs differently expressed among colon and rectum or higher in late stage tumors were identified. Expression levels of miR-31 were correlated to that of CA199 and miR-18a, miR-21 and miR-31 were associated with mutations in APC gene. Gene target predictions of these miRNAs pointed mainly the pathways related to CRC progression such as Wnt/TGFb, MAPK and TP53 signaling pathways. In addition, many of these miRNAs were predicted to target genes in the Vogelstein model. This study has identified promising miRNAs to be used as biomarkers (for example in plasma) in diagnostic and prognostic settings of CRC. And, in addition it shows their direct involvement in the regulation of driving events of CRC carcinogenesis which should be explored further in biological models. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4038.
SC-10 * DIVERGENT LIPID METABOLISM DRIVES SELF-RENEWAL IN GLIOBLASTOMA
Authors: J. Hale, T. Gromovsky, H. Alex Brown, J. Mark Brown, J. Lathia
Journal: Neuro-Oncology (2014)

The tumor microenvironment is essential to cancer stem cell (CSC) maintenance, providing discreet cues influencing cellular behavior. However, the mechanisms by which CSCs sense and respond to microenvironmental stresses, such as lipid availability, which regulates cellular metabolism and membrane turnover, are poorly understood. Scavenger receptors, a broad class of membrane receptors, are instrumental in the recognition and uptake of modified lipids, presenting a unique link between CSCs and the tumor microenvironment. Recent work published by our group utilizing patient-derived CSCs demonstrated elevated levels of the scavenger receptor CD36 in Glioblastoma (GBM) CSCs, with functional consequences on self-renewal and tumorigenesis. These results highlighted enhanced oxidized low-density lipoprotein uptake and utilization by CSCs and suggest that lipid metabolism is intimately linked to CSC maintenance. We therefore hypothesize that CSCs possess increased ability to take up and metabolize lipid species. We have observed increased lipid uptake and incorporation in CSCs as assessed by 14C acetate and 3H oleate. Despite increased uptake, CSCs show decreased lipid droplet accumulation as evidenced by Oil Red O staining. These results were confirmed by flow cytometry with the alternative lipid stain Nile Red. A metabolic shRNA screen is currently underway to identify potential targets in the regulation of lipid metabolism in CSCs. These data provide a paradigm by which CSCs utilize lipid species, providing survival and metabolic advantages and thereby allowing for tumor progression.
T1-MAPPING BY CARDIAC MAGNETIC RESONANCE IMAGING: FROM HISTOLOGICAL VALIDATION TO CLINICAL IMPLICATION
Authors: Andreas Anselm Kammerlander, Beatrice A. Marzluf, Caroline Zotter-Tufaro, Stefan Aschauer, Franz Duca, Alina Bachmann, Klaus Knechtelsdorfer, Matthias Wiesinger, Stefan Pfaffenberger, Andreas Greiser, Irene Lang, Diana Bonderman, Julia Mascherbauer
Journal: Journal of the American College of Cardiology (2016)
Abstract B33: Transcriptional control of genome surveillance and repair by a metabolic switch
Authors: Li-Jun Di, Madeline M. Wong, Clay T. Wakano, Jung Byun, Lyuba Varticovski, Kent Hunter, Olufunmilayo I. Olopade, Kevin Gardner
Journal: Cancer Research (2011)

Abstract A hallmark of primitive and more aggressive breast cancer is increased genome instability due to deficits in DNA repair. Recently we described a molecular mechanism through which the NADH regulated protein C-terminal binding protein (CtBP) represses the expression of the Breast Cancer One tumor suppressor gene BRCA1. These initial observations provided a molecular mechanism through which specific aspects of genome repair and stability, mediated by BRCA1, could be controlled by cellular metabolic status. By profiling the global interactions of CtBP with the breast cancer genome via chromatin immuno-precipitation combined with deep sequencing (ChlP-seq), we now reveal that CtBP acts both as a co-transcriptional repressor and activator to integrate distinct pathways that control genome stability, epithelial to mesenchymal transition, and stem cell transcriptional programming. Breast cancer cells depleted of CtBP show increased DNA repair as detected by comet and gamma H2AX foci formation assays. Over-expression of CtBP shows loss of the CD24 luminal surface marker while CtBP depletion increases CD24 expression. Kaplan-Meier survival analysis, based on our CtBP targeted gene lists, distinguishes breast cancer patients with worst outcome/survival in several published gene expression studies. Finally, tissue micro-array studies reveal significantly higher staining of CtBP predominantly in patients with poorly differentiated, triple negative and basal-like morphologic and molecular phenotypes. We propose a model in which CtBP acts at multiple levels to enhance dedifferentiation and genomic instability in malignancies of the breast. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the Second AACR International Conference on Frontiers in Basic Cancer Research; 2011 Sep 14-18; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2011;71(18 Suppl):Abstract nr B33.
AB0515 Salivary Alpha-Amylase and Antichromatin Antibody Are Elevated and Associated with Disease Activity in Sle
Authors: H.-A. Kim, J.-Y. Jung, J.-Y. Nam, S.-S. Kim, C.-H. Suh
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Psychological stress has been known as one of triggering factors in systemic lupus erythematosus (SLE). Saliva can be obtained easily without any invasive or painful procedure. Previously it has not been studied to measure disease related markers in saliva in patients with SLE. <h3>Objectives</h3> We investigated the salivary stress hormones, disease related biomarkers and two questionnairs about stress and depression among SLE patients and healthy controls. <h3>Methods</h3> Saliva were collected from patients with SLE (n=110) and healthy normal controls (NC, n=59). Salivary cortisol, α-amylase, interleukin-1β (IL-1β) and antichromatin antibody were measured by enzyme-linked immunosorbent assay. Also, perceived stress scale (PSS) and Beck depression index (BDI) were surveyed from all participants. <h3>Results</h3> The mean levels of salivary α-amylase were higher in SLE than in the NC (128.09±60.61 U/ml vs 101.56±54.30 U/ml, <i>p</i> =0.004) Also, antichromatin antibody was higher (50.7±114.3 AU vs 14.1±17.2 AU, <i>p</i> =0.002). However, those of cortisol and IL-1β were not different between the SLE and the NC. The mean value of BDI was more increased in the SLE than that in the NC (10.86±9.25 vs 5.37±4.82, <i>p</i> &lt;0.001), but there was no difference in the PSS. Salivary α-amylase was correlated with only ESR, and salivary IL-1β was correlated with BDI, disease duration, and ESR. Salivary antichromatin antibody was correlated with oral ulcer, and leukocyte count. SLE patients whose SLEDAI has been elevated had higher PSS scores and high titers of salivary antichromatin antibody 4 or 5 months before. <h3>Conclusions</h3> Salivary α-amylase and antichromatin antibody were elevated in patients with SLE. The SLE patients were suggested be more depressed than normal healthy control. There was no difference in recognizing stress, however, the activity of SLE became worse with increasing perceived stress levels. <h3>References</h3> Kozora E, Ellison MC, Waxmonsky JA, Wamboldt FS, Patterson TL. Major life stress, coping styles, and social support in relation to psychological distress in patients with systemic lupus erythematosus. Lupus 2005; 14:363-372. Van der Goes MC, Bossoma ER, Hartkamp A, Godaert GL, Jacobs JW, Kruize AA et al. Cortisol during the day in patients with systemic lupus erythematosus or primary Sjogren9s syndrome. J Rheumatol 2011; 38;285-288. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3473