tbuckley/cabot-search huggingface dataset:
Highly Educated Spouses and Physician Practice in Rural Areas—Reply
Authors: Douglas O. Staiger
Journal: JAMA (2016)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
Authors: Natalie Landeck, Hélène Hall, Mustafa T. Ardah, Nour K. Majbour, Omar M. A. El-Agnaf, Glenda Halliday, Deniz Kirik
Journal: Molecular Neurodegeneration (2016)

Alpha-synuclein (asyn) has been shown to play an important role in the neuropathology of Parkinson's disease (PD). In the diseased brain, classic intraneuronal inclusions called Lewy bodies contain abnormal formations of asyn protein which is mostly phosphorylated at serine 129 (pS129 asyn). This suggests that post-translational modifications may play a role in the pathogenic process. To date, several uniplex assays have been developed in order to quantify asyn not only in the brain but also in cerebrospinal fluid and blood samples in order to correlate asyn levels to disease severity and progression. Notably, only four assays have been established to measure pS129 asyn specifically and none provide simultaneous readout of the total and pS129 species. Therefore, we developed a sensitive high-throughput duplex assay quantifying total and pS129 human asyn (h-asyn) in the same well hence improving accuracy as well as saving time, consumables and samples. Using our newly established duplex assay we measured total and pS129 h-asyn in vitro showing that polo-like kinase 2 (PLK2) can phosphorylate asyn up to 41 % in HEK293 cells and in vivo the same kinase phosphorylated h-asyn up to 17 % in rat ventral midbrain neurons. Interestingly, no increase in phosphorylation was observed when PLK2 and h-asyn were co-expressed in rat striatal neurons. Furthermore, using this assay we investigated h-asyn levels in brain tissue samples from patients with PD as well as PD dementia and found significant differences in pS129 h-asyn levels not only between disease tissue and healthy control samples but also between the two distinct disease states especially in hippocampal tissue samples. These results demonstrate that our duplex assay for simultaneous quantification is a useful tool to study h-asyn phosphorylation events in biospecimens and will be helpful in studies investigating the precise causative link between post-translational modification of h-asyn and PD pathology.
Feasibility and efficacy of concomitant chemoradiation (Ch-RT) in the management of newly diagnosed elderly glioblastoma (GB) patients: Results from the GLIOCAT study.
Authors: Maria Martinez-Garcia, Estela Pineda, Sonia del Barco, Natalia Garcia, Eugenia Verger, Carlos Mesia, Anna Estival, Oscar Gallego, Miguel J. Gil Gil, Rafel Fuentes, Jordi Craven, Salvador Villà, Núria de la Iglesia, Francesc Alameda, Cristina Carrato, Teresa Ribalta, Jose Maria Velarde, Carmen Balana
Journal: Journal of Clinical Oncology (2016)

2045 Background: Elderly GB patients have a dismal prognosis. The optimal treatment in this population remains to be established. Methods: We performed a multicenter retrospective and prospective study of newly diagnosed GB patients (pts) treated with standard Ch-RT (Stupp regimen). In the present substudy we analyzed clinical outcome and prognostic factors in elderly ( > 65 y) pts and compare these to younger pts from the global cohort. Results: Between 2005 to 2014, 432 pts were enrolled. There were 148 > 65y; 117 (79%) 65-75y and 31 (21%) > 75y with a mean age of 72y, mean KPS 80%, and 58.8% were males. In pts > 65y, gross total resection (GTR) was performed in 34 (24.5%), and biopsy in 29 (20.9%) with postoperative complications in 17 (11.5%). The time to initiate Ch-RT was 4.93 weeks (w); 127 (85.8%) finished Ch-RT and 109 (73.6%) completed adjuvant temozolomide (TMZ). Only 16 (10.8%) could discontinue steroids during the concomitant therapy. There were 28 (18.9%) patients with pseudoprogression. MGMT methylation status was studied in 103 pts of which 57 (55.3%) were methylated. The only statistical differences between > 65 and younger pts were: lower KPS (p = 0.01) and fewer pts initiating adjuvant TMZ (p = 0.015). Median follow-up is 16.76 months. In the global population progression-free survival (PFS) and overall survival (OS) were 8 months (95% CI, 7.49-8.5) and 15 months (95% CI, 7.49-8.5), respectively, compared to 7 (95% CI, 6.1-7.8), and 11 months (95% CI, 8.7-13.2) in > 65y (p < 0.001 and p = 0.033). For elderly pts, on multivariate analysis, GTR and methylated MGMTbut not KPS were independent predictors of OS and PFS, respectively. Conclusions: Standard Ch-RT is feasible in elderly pts even though this population had poor KPS and fewer pts that could start adjuvant TMZ. As expected, PFS and OS were poorer in > 65y. For elderly patients, type of resection and MGMT methylation remain the more relevant prognostic factors in newly diagnosed GB treated with Ch-RT and adjuvant TMZ. Randomized prospective trials are warranted.
Humanized Mouse Xenograft Models: Narrowing the Tumor–Microenvironment Gap
Authors: J. Jason Morton, Gregory Bird, Yosef Refaeli, Antonio Jimeno
Journal: Cancer Research (2016)

Abstract Cancer research has long been hampered by the limitations of the current model systems. Both cultured cells and mouse xenografts grow in an environment highly dissimilar to that of their originating tumor, frequently resulting in promising treatments that are ultimately clinically ineffective. The development of highly immunodeficient mouse strains into which human immune systems can be engrafted can help bridge this gap. Humanized mice (HM) allow researchers to examine xenograft growth in the context of a human immune system and resultant tumor microenvironment, and recent studies have highlighted the increased similarities in attendant tumor structure, metastasis, and signaling to those features in cancer patients. This setting also facilitates the examination of investigational cancer therapies, including new immunotherapies. This review discusses recent advancements in the generation and application of HM models, their promise in cancer research, and their potential in generating clinically relevant treatments. This review also focuses on current efforts to improve HM models by engineering mouse strains expressing human cytokines or HLA proteins and implanting human bone, liver, and thymus tissue to facilitate immune cell maturation and trafficking. Finally, we discuss how these improvements may help direct future HM model cancer studies. Cancer Res; 76(21); 6153–8. ©2016 AACR.
SETDB2 Links Glucocorticoid to Lipid Metabolism through Insig2a Regulation
Authors: Manuel Roqueta-Rivera, Ryan M. Esquejo, Peter E. Phelan, Katalin Sandor, Bence Daniel, Fabienne Foufelle, Jun Ding, Xiaoman Li, Sepideh Khorasanizadeh, Timothy F. Osborne
Journal: Cell Metabolism (2016)
Empiric Definition of Eligibility Criteria for Clinical Trials in Relapsed/Refractory AML: Analysis of 1,892 Patients from HOVON/SAKK and SWOG
Authors: Roland B. Walter, Megan Othus, Bob Löwenberg, Gert J Ossenkoppele, Stephen H. Petersdorf, Thomas Pabst, Marie-Christianne Vekemans, Frederick R. Appelbaum, Harry P. Erba, Elihu H. Estey
Journal: Blood (2014)
Anti-CD38 Pretargeted Radioimmunotherapy Eradicates Multiple Myeloma Xenografts In a Murine Model
Authors: Damian J. Green, Jon C. Jones, Mark D. Hylarides, Donald K. Hamlin, D Scott Wilbur, Yukang Lin, Aimee L. Kenoyer, Shani L. Frayo, William I. Bensinger, Ajay K. Gopal, Johnnie J. Orozco, Theodore A. Gooley, Brent L. Wood, John M. Pagel, Oliver W. Press
Journal: Blood (2013)
The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children
Authors: Francine C. van Erp, Edward F. Knol, Bo Pontoppidan, Yolanda Meijer, Cornelis K. van der Ent, André C. Knulst
Journal: Journal of Allergy and Clinical Immunology (2016)
Coronary Physiology During Exercise and Vasodilation in the Healthy Heart and in Severe Aortic Stenosis
Authors: Matthew Lumley, Rupert Williams, Kaleab N. Asrress, Satpal Arri, Natalia Briceno, Howard Ellis, Ronak Rajani, Maria Siebes, Jan J. Piek, Brian Clapp, Simon R. Redwood, Michael S. Marber, John B. Chambers, Divaka Perera
Journal: Journal of the American College of Cardiology (2016)
Effect of zoledronic acid acts on the interaction between mesenchymal stem cells and breast cancer cells within the bone microenvironment.
Authors: N. Normanno, M. Gallo, L. Lamura, A. De Luca
Journal: Journal of Clinical Oncology (2010)

10602 Background: Zoledronic acid (ZA) has been recently shown to increase the progression free-survival of estrogen receptor (ER)-positive breast cancer patients by reducing both loco-regional and distant metastases. However, ZA rapidly concentrates into the bone following I.V. administration and it is unlikely that this drug has a direct effect on cell populations outside the bone. Bone-marrow-derived mesenchymal stem cell (MSCs) have been shown to increase the metastatic potential of breast cancer cells by secreting the chemokine RANTES (CCL5) that sustains breast cancer motility and invasion. Methods: The antiproliferative effects of ZA on MSCs were evaluated with an anchorage-dependent growth assay. Migration of breast cancer cells through a fibronectin-coated membrane was evaluated by using a commercially available assay. The effects of ZA on the secretion of cytokines were assessed by using the Bio-Plex Suspension Array. Results: Treatment with 20 μM ZA produced marginal effects on the growth of MSCs (approximately 25% growth inhibition), but significantly impaired the ability of these cells to sustain breast cancer migration. In fact, conditioned medium from ZA-treated MSCs showed a reduced ability to promote the migration of ER-positive MCF-7 breast cancer cells as compared with conditioned medium from untreated cells. Analysis of conditioned media from MSCs revealed that treatment with ZA almost completely suppressed the ability of MSCs to secrete RANTES (>90% reduction) and IL-6 (50% reduction) that has been shown to act as a potent paracrine growth factor for MCF-7 cells. Treatment with anti-RANTES and anti-IL-6 antibodies, alone or in combination, produced a significant reduction of the migration of MCF-7 induced by conditioned medium from untreated MSCs. Finally, in co-culture assays, treatment with ZA reduced the ability of MSCs to sustain the growth of MCF-7 cells. Conclusions: These results demonstrate that ZA might exert its antitumor activity in the bone marrow microenvironment by blocking the ability of MSCs to secrete factors involved in breast cancer migration and proliferation. No significant financial relationships to disclose.
Intestinal Microbiota and Health of Adults Who Were Born by Cesarean Delivery
Authors: James J. Goedert
Journal: JAMA Pediatrics (2016)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Mycological Diagnosis of Invasive Pulmonary Aspergillosis Should Depend on Both the Underlying Condition and the Neutropenia Status of Patients with Hematologic Malignancies
Authors: Anne Bergeron, Raphaël Porcher, Annie Sulahian, Cédrid de Bazelaire, Karine Chagnon, Claire Lacroix, Emmanuel Raffoux, Françoise Isnard, Jean-Louis Poirot, Anne Vekhoff, Claire Bouges-Michel, Abdellatif Tazi, Patricia Ribaud
Journal: Blood (2010)
Gastric linitis: Results of a French national survey confirming the specificity of this gastric neoplasm and the limited therapeutic options.
Authors: M. Ducreux, O. Bouche, C. Lombard-Bohas, S. Manfredi, D. Pezet, J. Giroux, I. Sobhani, R. Coriat, V. Palascak, P. Burtin
Journal: Journal of Clinical Oncology (2010)

e14581 Background: The gastric linitis is a rare tumor of the stomach; the incidence appears to be growing and evolving profile specific. To gain a more comprehensive picture of this rare disease, we report here the results of a French national survey conducted between 2005 and 2007. Methods: Were included all patients with gastric linitis (prevalence survey). A predetermined form, widely distributed was used by the centers participating in the study. Results: 116 records have been received during the inclusion period; there were 60 men and 56 women of average age 60.5 years (24-83). The linitis involved the entire stomach in 24 patients, it was limited to two thirds of the stomach in 18, limited to the cardia in 9, the upper third of the stomach in 10, the middle third in 19, the lower third in 35. The disease was metastatic in 45 cases and limited in 61. The metastases were located in the peritoneum: 37 cases, in lymph nodes: 6, in bone: 4, 2 patients had bone marrow infiltration, 3 had liver metastases, 2 ovarian, 2 lung metastases and one had metastatic pleural effusion. Surgical excision of the primary was performed in 73 cases. Among the 61 patients with localized disease, R0 resections was done only in 44, R1 in 10 and R2 in 2. Adjuvant chemotherapy was done in 14 patients, postoperative chemoradiotherapy in 22. Tumor recurrence after curative resection was observed in 25 patients (during the follow-up period ranging from 2 months to 6 years), among them 20 were peritoneal recurrences. Their time of onset ranged from 4 to 70 months. Among the metastatic patients, 25 received chemotherapy, 14 had 2 lines, 10 had 3 lines, one had 4 lines and one had 5 lines. The date of death is available only for 40 patients with extremes ranging from 2 weeks to 86 months. Complete and updated survival data will be presented at the congress. Conclusions: This French national Survey confirms that gastric linitis sometimes reaches young subjects. 50% could be treated with curative intent, but recurrence is the rule, especially in the peritoneum. Relapses can be observed more than 5 years after curative treatment. Some metastasized patients survive long enough to receive multiple lines of chemotherapy. Author Disclosure Employment or Leadership Position Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration GlaxoSmithKline Amgen, Roche Amgen, Ipsen, Merck Serono, Novartis, Pfizer, Roche Roche
Grey matters; on the importance of publication bias in systematic reviews
Authors: Marinus Winters, Adam Weir
Journal: British Journal of Sports Medicine (2016)

‘How can I treat my patient best?’ is a question clinicians ask themselves on a daily basis. For clinical inquiries, systematic reviews (SRs) deliver the highest level of evidence in medical sciences.1 They can cover a variety of domains, including treatment and diagnostics. Presenting a full overview of the literature and delivering an unbiased estimate of effect are key aims of SRs. In this editorial, we explore publication bias, one of the potential sources of bias in SRs.

Authors of SRs usually search multiple databases to find relevant publications. However, is all the available evidence identified with this approach? Publication bias is one threat to delivering an unbiased effect estimate.2 ,3 Publication bias is a phenomenon where journals are more likely to publish positive results, as these articles yield more citations. Non-significant publications are more prone to be rejected and remain unpublished.4 Unpublished resources are known as ‘grey literature’.

Publication bias has been shown in …
Association of Dietary Intake and Biomarker Levels of Arsenic, Cadmium, Lead, and Mercury among Asian Populations in the United States: NHANES 2011–2012
Authors: Hiroshi Awata, Stephen Linder, Laura E. Mitchell, George L. Delclos
Journal: Environmental Health Perspectives (2016)

We have recently shown that biomarker levels of selected metals are higher in Asians than in other U.S. ethnic groups, with important differences within selected Asian subgroups. Much of this difference may be dietary in origin; however, this is not well established.We evaluated dietary intake of toxic metals as a source of increased biomarker levels of metals among U.S. Asians.We estimated daily food consumption and dietary intake of arsenic, cadmium, lead, and mercury by combining 24-hr dietary intake recall data from the 2011-2012 National Health and Nutrition Examination Survey (NHANES) with data from the USDA Food Composition Intake Database and FDA Total Dietary Study. We analyzed associations between dietary metal intake and biomarker levels of the metals using linear regression. Further, estimated food consumption and metal intake levels were compared between Asians and other racial/ethnic groups (white, black, Mexican American, and other Hispanic) and within three Asian subgroups (Chinese, Indian Asian, and other Asians).Significant associations (p < 0.05) were found between biomarker levels and estimated dietary metal intake for total and inorganic arsenic and mercury among Asians. Asians had the highest daily fish and rice consumption across the racial/ethnic groups. Fish was the major contributor to dietary mercury and total arsenic intake, whereas rice was the major contributor to inorganic arsenic dietary intake. Fish consumption across the Asian subgroups varied, with Asian Indians having lower fish consumption than the other Asian subgroups. Rice consumption was similar across the Asian subgroups.We confirmed that estimated dietary intake of arsenic (total and inorganic) and mercury is significantly associated with their corresponding biomarkers in U.S. Asians, using nationally representative data. In contrast, estimated dietary intake of cadmium and lead were not significantly associated with their corresponding biomarker levels in U.S. Asians. Citation: Awata H, Linder S, Mitchell LE, Delclos GL. 2017. Association of dietary intake and biomarker levels of arsenic, cadmium, lead, and mercury among Asian populations in the United States: NHANES 2011-2012. Environ Health Perspect 125:314-323; http://dx.doi.org/10.1289/EHP28.
Conjunctival allergen provocation test : guidelines for daily practice
Authors: J.-L. Fauquert, M. Jedrzejczak-Czechowicz, C. Rondon, V. Calder, D. Silva, B. K. Kvenshagen, I. Callebaut, P. Allegri, N. Santos, S. Doan, D. Perez Formigo, F. Chiambaretta, L. Delgado, A. Leonardi
Journal: Allergy (2016)

Conjunctival allergen provocation test (CAPT) reproduces the events occurring by instilling an allergen on the ocular surface. This paper is the compilation of a task force focussed on practical aspects of this technique based on the analysis of 131 papers. Main mechanisms involved are reviewed. Indications are diagnosing the allergen(s)-triggering symptoms in IgE-mediated ocular allergy in seasonal, acute or perennial forms of allergic conjunctivitis, especially when the relevance of the allergen is not obvious or in polysensitized patients. Contraindications are limited to ongoing systemic severe pathology, asthma and eye diseases. CAPT should be delayed if receiving systemic steroids or antihistamines. Local treatment should be interrupted according to the half-life of each drug. Prerequisites are as follows: obtaining informed consent; evidencing of an allergen by skin prick tests and/or serum-specific IgE dosages; being able to deal with an unlikely event such as acute asthma exacerbation, urticaria or anaphylaxis, or an exacerbation of allergic conjunctivitis. Allergen extracts should be diluted locally prior to administration. Positive criteria are based on itching or quoted according to a composite score. An alternative scoring is based on itching. CAPT remains underused in daily practice, although it is a safe and simple procedure which can provide valuable clinical information.
Error-Prone DNA Polymerases iota and beta Are Over-Expressed in B-CLL Cells: Correlation with Specific IgVH Point-Mutations and Implication for the Pathogenesis of Intraclonal IgVH Diversification.
Authors: Valter Gattei, Massimo Degan, Michele Dal Bo, Antonella Zucchetto, Elena Betto, Carlo Pucillo, Giovanni Del Poeta, Alfonso Colombatti, Riccardo Bomben
Journal: Blood (2004)
Final Results from a Phase 4 Open-Label 3-Year Study Evaluating Changes in Bone Marrow (Bm) Morphology in Adult Immune Thrombocytopenia (ITP) Patients (Pts) Receiving the Thrombopoietin Mimetic Romiplostim
Authors: Ann Janssens, Francesco Rodeghiero, David Anderson, Beng Chong, Zoltan Boda, Ingrid Pabinger, Libor Cervinek, Xuena Wang, Angela Lopez
Journal: Blood (2014)
IgG Alloantibodies Against RBC Induced By Pregnancy or Transfusion Have Unique Glycosylation Patterns Which Correlate with Clinical Outcome of Hemolytic Disease of the Fetus or Newborn
Authors: Myrthe Elisabeth Sonneveld, Rosina Plomp, Jon Admiraal, Carolien Koeleman, Agnes Hipgrave-Ederveen, Joke Koelewijn, Peter Ligthart, Masja De Haas, Manfred Wuhrer, C. Ellen Van der Schoot, Gestur Vidarsson
Journal: Blood (2015)
Recurrent venous thromboembolism in patients with pulmonary embolism and right ventricular dysfunction: a post-hoc analysis of the Hokusai-VTE study
Authors: Marjolein P A Brekelmans, Walter Ageno, Ludo F Beenen, Benjamin Brenner, Harry R Buller, Cathy Z Chen, Alexander T Cohen, Michael A Grosso, Guy Meyer, Gary Raskob, Annelise Segers, Thomas Vanassche, Peter Verhamme, Philip S Wells, George Zhang, Jeffrey I Weitz
Journal: The Lancet Haematology (2016)
P0145 : Prospective analysis of factors implicated in early readmissions in patients with decompensated cirrhosis
Authors: L. Franco, J.A. Ferrusquía, S. Antón, A. Ríos, C. Fernández de Castro, O.K. Nuevo, L. González-Diéguez, V. Cadahía, M. Varela, C.A. Navascués, J. de la Vega, M. Rodríguez
Journal: Journal of Hepatology (2015)
Chest Discomfort and Longstanding Dyspnea on Exertion
Authors: Ryan A. Macke, Thomas P. Templin, Justin D. Blasberg
Journal: JAMA Surgery (2016)

A man in his early 40s was referred for surgical evaluation following abnormal imaging obtained during workup of recent left-sided chest discomfort and longstanding dyspnea on exertion. What is your diagnosis?