tbuckley/cabot-search huggingface dataset:
Abstract 2268: Systems analysis identified Thioredoxin family genes as a prognostic signature and novel therapeutic target for gastric cancer
Authors: Jae Yun Lim, Sun Och Yoon, Soon Won Hong, Jong Won Kim, Seung Ho Choi, Jae Yong Cho
Journal: Cancer Research (2011)

Abstract Background: Gastric cancer is the second cause of cancer-related deaths worldwide. After curative surgery, relapse occurs in a large number of patients except for early gastric cancer patients. Even though within the same stage, gastric cancer patients present diverse clinical manifestations and prognosis. The clinical diversity of gastric cancer arises from the molecular biological diversity, which is caused by changes in different genes. This study aimed to identify potential biomarkers which could be used to classify patients according to prognosis and also become new therapeutic targets. Procedures: We obtained whole genome expression microarray data of 65 primary gastric adenocarcinoma patients and the results were validated by quantitative reverse transcription polymerase chain reaction experiment of 68 gastric cancer tissues. We uncovered prognostic subgroups of gastric cancer and identified expressions of a limited number of genes are significantly associated with prognosis. Immunohistochemical assay using 328 gastric cancer tissues was performed to validate protein levels of biomarker gene. Results: A gene encoding thioredoxin (TXN) was found to be markedly elevated in many gastric cancer tissues and strongly related to poor prognosis (p=0.009) in univariate analysis. Conversely, thioredoxin interacting protein (TXNIP), TXN inhibitor, was significantly decreased in patients with poor prognosis. Systems analysis revealed TXN and TXNIP were highly correlated with many oncogenes and tumor suppressors and also with energy and protein synthesis. It was validated that TXN and TXNIP expression were significant prognostic biomarker even in the same stage patients. When patients were classified into the three groups by expression level of TXN and TXNIP, TXN low and TXNIP high expression group showed better prognosis than others; recurrence free survival (p&amp;lt;0.001) and overall survival (p=0.001). Hypoxia induced gene, ERO1-like (ERO1L), also highly correlated with TXN and this suggested that TXN and TXNIP were influenced by hypoxia in gastric cancer. Conclusion: TXN and TXNIP are promising prognostic markers for gastric cancer and agents that target TXN or up modulate TXNIP could be used in target therapy for gastric cancer patients who are selected based on TXN and TXNIP gene signatures. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2268. doi:10.1158/1538-7445.AM2011-2268
OC-093 A pragmatic multicentre randomised controlled trial comparing stapled haemorrhoidopexy to traditional excisional surgery for haemorrhoidal disease. the ethos trial
Authors: AJ Watson, H Bruhn, K Macleod, A McDonald, G McPherson, M Kilonzo, J Norrie, MA Loudon, K McCormack, J Wood, JA Cook
Journal: Gut (2015)

<h3>Introduction</h3> Haemorrhoidal disease is common in all age groups from the mid-teens onwards. Approximately 25,000 procedures were performed in England during 2006/2007 on hospital day-cases or inpatients.<sup>1</sup>Current interventions include traditional excisional surgery (TH) and stapled haemorrhoidopexy (SH). Uncertainty remains about how they compare.<sup>2–6</sup> The primary aim of eTHoS is to assess whether there is a difference between SH and TH<sup>7 </sup>for the treatment of haemorrhoidal disease (grades II, III, and IV) on patient centred quality of life profile. Treatment costs will also be examined. <h3>Method</h3> Participants were randomly allocated to one of two study groups in equal proportion (SH or TH). Thirty-one sites from within the UK took part in the study. Patients, aged 18 year or older, with a diagnosis of circumferential haemorrhoids grade II–IV were eligible to take part. Randomisation utilised a minimisation algorithm which incorporated centre, grade of haemorrhoid (s), baseline EuroQol 5 Dimension health status questionnaire (EQ-5D) score and gender. The primary outcome for this study is patient-centred, quality of life profile (area under the curve (AUC) derived from EQ-5D data collected at baseline, 1 week, 3 weeks, 6 weeks, 12 months and 24 months; Economic, incremental cost per quality adjusted life year (QALY) gained with QALYs were based on the responses to the EQ-5D. A sample size of n = 338 per group was calculated to provide 90% power to detect a difference in the mean area under the quality of life curve of 0.25 standard deviations (derived from EQ-5D score measurements, with a significance level of 5%). 400 were to be randomised to each group. <h3>Results</h3> The eTHoS trial closed for recruitment in August 2014 and is in follow-up. A total of 1129 patients were approached to take part in the trial. 777 participants were randomised (388 to TH and 389 to SH) with 7 post-randomisation exclusions from each group. <h3>Conclusion</h3> This trial is currently the largest of its type in the world. Our results will be reported in full in October 2016 to The National Institute for Health Research. <h3>Disclosure of interest</h3> None Declared. <h3>References</h3> NHS Information Centre UK; http://www.hesonline.nhs.uk/servlet/ContentServer?siteID=1937&amp;catergoryID-215 Shao WJ, <i>et al</i>. Br J Surg. 2008;95(2):147–60 Jayaraman S, <i>et al</i>. Dis Colon Rectum. 2007;50(9):1297–305 Burch J, <i>et al</i>. Health Technol Assess. 2008;12(8) Thaha MA, <i>et al</i>. Gut 2009;58(5):668–78 Shanmugam, et alet al. Colorectal Dis. 2010;12:579–86 Watson, et alet al. Trials 2014;15:439
THE ROLE OF MYELOID DERIVED SUPPRESSOR CELLS (MDSCS) IN TUMOR-INDUCED IMMUNOSUPPRESSION IN HUMAN AND MURINE GLIOMAS
Authors: M. A. Vogelbaum, B. Otvos, B. Raychaudhuri, D. Hambardzumyan, J. Finke, J. Lathia
Journal: Neuro-Oncology (2014)

BACKGROUND: (blind field) METHODS: To further study the biology of MDSCs in GBM, we have evaluated two preclinical models of GBM (RCAS tVA, GL261 orthografts) and observed a similar magnitude of glioma-induced elevation of circulating MDSCs as we observed in GBM patients. RESULTS: With use of immunofluorescent, antibody-based cellular labeling techniques, we have identified MDSCs within primary human and murine glioma tumor samples, and started to identify the niche to which MDSCs hone within these tumors. Further, we have observed an immunosuppressive phenotype marked by staining of MDSCs for arginase I, a known mediator capable of reducing cytotoxic T cell suppressor function. We also have observed co-localization between MDSCs and cancer stem cells (CSCs) in both human tissues and mouse models, leading to the hypothesis that CSCs recruit MDSCs to the tumor microenvironment, promote their survival, and that MDSCs are at least in part responsible for the immune-evasive properties of CSCs. Growth of gliomas in mice has been associated with expansion of the arginase-producing subset of MDSCs within bone marrow, and increased levels of arginase-secreting MDSCs within the glioma microenvironment. In vitro studies with isolated murine MDSCs have demonstrated that CSC conditioned media promotes decreased apoptosis and increased arginase production by these cells. Finally, treatment of glioma-bearing mice with sunitinib, a signal transduction inhibitor that has established anti-MDSC properties, resulted in a reduction in circulating and tumor-infiltrating MDSCs, increased T cell infiltration, and prolonged survival. CONCLUSIONS: Taken together, these results suggest a critical role by which gliomas are able to promote immunosuppression through the recruitment of MDSCs via CSCs into tumors and subsequent interaction of recruited MDSCs with tumor-infiltrating T cells. SECONDARY CATEGORY: n/a.
Abstract 3055: Wnt inhibitory factor 1 inhibits cervical cancer cell growth <i>in vitro</i>
Authors: Ilangovan Ramachandran, Satish Ramalingam, Gopalan Natarajan, Wilbur K. Mills, Shrikant Anant, Antonio M.C. Reis, Lurdes Queimado
Journal: Cancer Research (2010)

Abstract Background and Purpose: Cervical cancer is one of the most common malignancies and major causes of death in women worldwide. Aberrant activation of Wnt/β-catenin pathway is associated with various cancers including cervical cancer. Mutations within intracellular components of the Wnt pathway are rare in cervical cancer. We hypothesize that alterations in secreted Wnt antagonists might play a major role in Wnt activation. Here we characterize the status of the Wnt inhibitory factor 1 (WIF1) in cervical cancer cell lines and determined the in vitro effects of WIF1 on cervical cancer cells. Methods: Three cervical cancer cell lines (HeLa, C33A and CC1) were treated with the demethylating agent 5-aza-2′-deoxycytidine (DAC) for 4 days, total RNA was isolated and real-time RT-PCR was performed to determine WIF1 mRNA expression. HeLa cells were transfected simultaneously with either pCI blast (empty vector) or pCI blast-WIF1 using LipoD293 transfection reagent and assessed for cell proliferation and apoptosis. Cell proliferation was determined at different time intervals (24, 48, 72 and 96 h) by hexosaminidase assay. Cell cycle analysis was performed after 72 h by flow cytometry using FACSCalibur analyzer. Caspase 3/7 activity was measured using the Apo-one Homogeneous Caspase-3/7 Assay kit. Results: We demonstrated that WIF1 is down-regulated in cervical cancer cells. Treatment with DAC caused a significant increase in WIF1 mRNA expression in all the cell lines tested compared with vehicle treatment. Furthermore, transfection with a plasmid expressing WIF1 significantly inhibited cervical cancer cell proliferation at all time points studied. To probe the possible mechanisms involved in WIF1 cell growth inhibition, we performed cell cycle analysis and caspase 3/7 assay. Interestingly, cell cycle analysis demonstrated that transfection with WIF1 significantly increased the G2-M cell population and the number of apoptotic cells. In addition, WIF1 significantly induced caspase 3/7 activity suggesting that WIF1 induced apoptosis is mediated through caspase 3/7 activation. Conclusions: Our data suggests that WIF1 is silenced in cervical cancer cells and WIF1 restoration inhibits cervical cancer cell growth by inducing G2-M arrest and apoptosis. This study emphasizes the importance of WIF1 as a potential therapeutic strategy in the treatment of cervical cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3055.
Incidental Detection of a Malignant Hurthle Cell Carcinoma by Tc-99m Sestamibi Cardiac Imaging
Authors: CHRISTINE FISHER, AMY VEHEC, BASHAR KASHLAN, GERALD LONGA, LOWELL HOUPT, KRISTI HOWE, LISA STARK, DENNIS CAVANAUGH
Journal: Clinical Nuclear Medicine (2000)

FISHER, CHRISTINE B.S.; VEHEC, AMY B.S.; KASHLAN, BASHAR M.D.; LONGA, GERALD M.D.; HOUPT, LOWELL C.N.M.T.; HOWE, KRISTI C.N.M.T.; STARK, LISA C.N.M.T.; CAVANAUGH, DENNIS M.D. Author Information
P541 QUERCETIN INDUCES HSC APOPTOSIS, UP-REGULATION OF CB2 AND DOWN-REGULATION OF CB1 IN AN ANIMAL MODEL OF LIVER FIBROSIS
Authors: L. Hernandez Ortega, A.A. Sobrevilla Navarro, A.M.M. Salazar Montes, J. Armendariz-Borunda
Journal: Journal of Hepatology (2014)
AB0177 Patterns and severity of lung disease in patients with rheumatoid arthritis
Authors: L. Yalakki Jagadeesh, B. Lams, R. Preston, N. Gullick, B. Kirkham, S. Agarwal
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Rheumatoid arthritis (RA) is a chronic autoimmune condition characterised by inflammatory polyarthritis, with lung disease being an important extra-articular manifestation. <h3>Objectives</h3> The primary aim of this study was to explore the different radiological patterns and severity of lung disease in patients with rheumatoid arthritis. A secondary aim was to determine predictors for the various patterns of lung disease. <h3>Methods</h3> A total of 90 patients with RA and lung disease were included in this retrospective cohort study. All patients had lung disease confirmed on high resolution computed tomography (HRCT). Radiological patterns of lung disease and their severity were scored independently by an experienced chest physician and a consultant radiologist and consensus was reached.5 patterns of lung disease were characterised– fibrosis, emphysema, large airway dilatation, large airway wall thickening and mosaicism (small airways disease) and graded as absent, mild, moderate or severe. <h3>Results</h3> 62 patients were female and 28 were male with a median age of 70 years(range 38-94 years). The mean duration of RA was 13.5 years, although 39 patients (43.3%) were diagnosed with lung disease within 1 year of diagnosis of RA. The majority of patients were receiving either oral steroids(96%) or methotrexate(88%) at the time of diagnosis of lung disease.80 patients were positive for rheumatoid factor (RF) and of the 41 patients with documented anti-citrullinated protein antibodies (ACPA),25 patients were positive. Radiological erosions were seen in 44 patients(48.9%) and extra-articular manifestations were seen in only 14 patients(15.6%). The incidence of the different lung patterns is shown in the graph. Large airway wall thickening was the commonest pattern observed(55.6%). Fibrosis emerged as the most severe radiological pattern(11.1%). Most patients had a mixed pattern of disease. There was no association between RF/ACPA positivity and the individual patterns of lung disease. DAS 28 showed a positive correlation with large airway wall dilatation at the time of diagnosis of lung disease. Female gender was identified as a predictor for mosaicism(p 0.01)and male gender as a predictor for fibrosis(p 0.03) Smoking is confirmed as a strong predictor for emphysema(p 0.002). <h3>Image/graph</h3> <h3>Conclusions</h3> In patients with RA a variety of radiological patterns of lung disease are seen. Large airway wall thickening was the commonest pattern identified in our cohort although the most severe radiological lung disease was due to fibrosis. <h3>Disclosure of Interest</h3> None Declared
Establishment of a pure NOTES procedure using a conventional flexible endoscope
Authors: E. Linghu
Journal: Endoscopy (2011)

Mori et al. [1] discuss the possibility of using a conventional flexible endoscope during pure natural orifice transluminal endoscopic surgery (NOTES) in patients with gastric gastrointestinal stromal tumors (GISTs), which is a really amazing idea. They use several novel techniques during full-thickness resection of GISTs, which may lay the foundation for the realization of pure NOTES using only conventional flexible endoscopes. First, a nasal endoscope was introduced to either create a perforation or assist in pulling up the collapsed gastric wall to prevent the operative view from being obscured. Second, a duodenal balloon was used to prevent gas from flowing beyond the outlet of the stomach and causing uncomfortable distention of the intestine. Third, unlike previous studies, perforation was made at 10-mm intervals instead of a continuous incision, a process that can also provide landmarks for resection of GISTs.
Efficacy and Safety of the SQ-House Dust Mite Sublingual Immunotherapy Tablet in North American Children and Adults: Findings from a Large Randomized, Placebo-Controlled Clinical Trial
Authors: Hendrik Nolte, David I. Bernstein, Joerg R. Kleine-Tebbe, Gordon L. Sussman, Dorthe Seitzberg, Dorte Rehm, Amarjot Kaur, Ziliang Li, Susan Lu, Harold S. Nelson
Journal: Journal of Allergy and Clinical Immunology (2016)
SAT0172 Psychiatric Disorders in Behcet’S Disease: is It a Primary or Secondary Feature of the Disease?
Authors: R. Talarico, E. Elefante, L. Palagini, A. d’Ascanio, C. Stagnaro, C. Ferrari, C. Tani, C. Baldini, M. Mosca, S. Bombardieri
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Frequency of psychiatric disorders in BD is a debated issue: while some experts attribute their presence to the chronicity of the illness, others think that they may be imputable to disease activity or to intrinsic features of the disease. <h3>Objectives</h3> The primary aims were to determine the frequency of psychiatric disorders in BD patients, both with neurological involvement or without; the secondary aims were: to investigate a possible association between disease activity/organ involvement and psychiatric profile of the BD patients and to compare the distribution of psychiatric disorders of patients with BD with those in patients with other chronic diseases. <h3>Methods</h3> One hundred and seven BD patients with a diagnosis of BD according the ISG criteria were studied. Demographic profile of the cohort studied are summarised in Table 1. Psychiatric disorders evaluated were: bipolar disorder, obsessive-compulsive disorder, depression and sleep disorder. Age and sex matched disease controls of systemic lupus erythematosus (SLE) and chronic arterial hypertension were included. <h3>Results</h3> Prevalence of psychiatric disorders are shown in Table 2. No correlations were found between the presence of psychiatric disorders and disease activity/organ involvement. Moreover, the frequency of bipolar disorder resulted significantly higher than in disease controls (p &lt; 0.001). <h3>Conclusions</h3> Our results show a high frequency of psychiatric disorders in BD patients. This elevated prevalence both in BD patient with or without neurological involvement, in presence or absence of disease activity and in a higher frequency than in disease controls, strongly suggest that BD patients are characterised by a specific psychiatric profile. <h3>Disclosure of Interest</h3> None Declared
Colonic stenting in patients on P2Y12 receptor antagonists and direct oral anticoagulants: are current BSG/ESGE guidelines practical?
Authors: Senthil V M Murugesan, Mark T Hendrickse
Journal: Gut (2016)

We thank the British Society of Gastroenterology and the European Society of Gastrointestinal Endoscopy for updating guidelines relating to endoscopy in patients on antiplatelets or anticoagulation therapy.1 However, we wish to raise two important issues. First, this guideline …
Effective Treatment of Experimental Glioblastoma by HSV Vector-Mediated TNFα and HSV-tk Gene Transfer in Combination with Radiosurgery and Ganciclovir Administration
Authors: Ajay Niranjan, Shusuke Moriuchi, L. Dade Lunsford, Douglas Kondziolka, John C. Flickinger, Wendy Fellows, Swaminathan Rajendiran, Masakazu Tamura, Justus B. Cohen, Joseph C. Glorioso
Journal: Molecular Therapy (2000)

<h2>Abstract</h2> Experiments were carried out in a nude mouse model of human glioblastoma to determine whether gamma-knife radiosurgery combined with herpes simplex virus thymidine kinase (tk) suicide gene therapy and tumor necrosis factor alpha (TNFα) gene transfer provided an improved multimodality treatment of this disease. Animals were inoculated intracerebrally with 2 × 10<sup>5</sup> U-87MG human glioblastoma cells to establish brain tumors. At 3 days postinoculation, the tumor region was injected with 2 × 10<sup>6</sup> infectious particles of highly defective herpes simplex viral vectors expressing the viral tk gene with the kinetics of a viral immediate early gene either alone (T.1) or together with TNFα (TH:TNF). Subgroups of animals were given daily intraperitoneal injections of ganciclovir (GCV) for 10 days and/or subjected to gamma-knife radiosurgery on the fifth day post tumor-cell implantation. Comparisons of animal survival showed that the TH:TNF vector in combination with radiosurgery and GCV administration provided the most effective therapy; eight of nine animals survived for 75 days compared to four of eight using the next best protocol. These findings suggest that gene therapy in combination with more conventional therapeutic methods may provide an improved strategy for extending the life expectancy of patients afflicted with this ultimately fatal disease.
Abstract 5398: TARDBP regulates glycolysis in hepatocellular carcinoma by regulating PFKP through miR-520.
Authors: Yun-Yong Park, Sang Bae Kim, Hee Dong Han, Bo-Hwa Sohn, Jiyong Liang, Yiling Lu, Gordon Mills, Anil Sood, Ju-Seog Lee
Journal: Cancer Research (2013)

Abstract Metabolic changes are common features of many cancer cells and are frequently associated with the clinical outcome of patients with various cancers including hepatocellular carcinoma (HCC). Thus, aberrant metabolic pathways in cancer cells are attractive targets for cancer therapy. However, our understanding of cancer-specific regulatory mechanisms of cell metabolism is still very limited. We found that Tat activating regulatory DNA-binding protein (TARDBP) is a novel regulator of glycolysis in HCC cells. TARDBP regulates expression of the platelet isoform of phosphofructokinase (PFKP), the rate-limiting enzyme of glycolysis that catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. Silencing of TARDBP expression in multiple HCC cell lines leads to impaired glucose metabolism and inhibition of in vitro and in vivo growth of HCC cells. Notably, the miR-520 family is an intermediate regulator of TARDBP-mediated regulation of glycolysis. Mechanistically, TARDBP suppressed expression of the miR-520 family, which in turn inhibited expression of PFKP. We further showed that expression of PFKP is significantly associated with the overall survival of patients with HCC. Our study provides new mechanistic insights into the regulation of glycolysis in HCC cells and reveals TARDBP as a potential therapeutic target for HCC. Citation Format: Yun-Yong Park, Sang Bae Kim, Hee Dong Han, Bo-Hwa Sohn, Jiyong Liang, Yiling Lu, Gordon Mills, Anil Sood, Ju-Seog Lee. TARDBP regulates glycolysis in hepatocellular carcinoma by regulating PFKP through miR-520. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5398. doi:10.1158/1538-7445.AM2013-5398
Tackling the Achilles' Heel of Hemodialysis
Authors: Wolfgang C. Winkelmayer
Journal: New England Journal of Medicine (2011)

More than 375,000 patients undergo long-term hemodialysis treatment in the United States, but the outcomes have remained abysmal, with the rate of death during the first year of hemodialysis therapy exceeding 20%. Although the development half a century ago of techniques for sustainable vascular access rendered long-term extracorporeal treatment feasible, vascular access remains the Achilles' heel of hemodialysis. The current options include arteriovenous fistulas, synthetic grafts, and central venous catheters, with a clear hierarchy among these options. Native arteriovenous fistulas — simple anastomoses of forearm arteries and veins — yield the best outcomes. Among otherwise similar patients, those with functioning . . .
Proton Nuclear Magnetic Resonance Spectroscopy of Plasma from Healthy Subjects and Patients with Cancer
Authors: Terje Engan, Jostein Krane, Olbjørn Klepp, Stener Kvinnsland
Journal: New England Journal of Medicine (1990)

To evaluate the ability of proton nuclear magnetic resonance (NMR) spectroscopy to indicate the presence or absence of malignant disease, we analyzed plasma samples from 104 patients with untreated cancer of various types and from 164 healthy controls. All specimens were coded with random numbers, and the investigators were blind to patient category. A statistically significant difference (P<0.001) was found between the mean (±SD) line widths in the plasma samples from the controls (39.1±6.7 Hz) and the line widths in plasma from the patients with cancer (35.2±6.4 Hz). However, the values showed considerable overlap between the two groups.