tbuckley/cabot-search huggingface dataset:
Abstract 567: Tumor suppressor merlin and WWOX involve in regulating tumor cell growth via epithelial mesenchymal transition (EMT)
Authors: Wan-Jou Shen, Yu-Feng Huang, Han-Yu Lin, Chun-I Sze, Nan-Shan Chang
Journal: Cancer Research (2011)

Abstract Cancer radiotherapy may complicated by chemo/radiation-induced resistance. Gene expression profiling studies have revealed that the tumor carries mesenchymal signature is a poor prognostic predictor. Our laboratory had generated post-irradiated 1306-MG glioblastoma (GBM) cell line which expresses epithelial mesenchymal transition (EMT) markers such as vimentin, fibronectin, desmin, and carbonic anhydrase III. Nuclear translocation of vimentin also occurs in these cells. These findings all indicate EMT. EMT requires cells transition and detachment which is mainly driven by a hyperactive Raf/MAPK pathway via transforming growth factor β (TGF-β) signaling. TGF-β signaling interacts with cell surface Hyal-2 and recruits WWOX. We treated 1306-MG with hyaluronan (HA) and co-transfected with wild type, active form, and inactive form merlin plus WWOX. By fluorescence resonance energy transfer (FRET) and co-immuoprecipitation (CO-IP) assays, our data showed that WWOX interacted with merlin in GBM cell line after treated with hyaluronan (HA). This finding suggest that WWOX participates in the regulation of CD44/merlin signaling. The growth of 1306-MG in merln or WWOX transfected cells showed more cells in S/G2M phases than co-tranfected cells. The co-tranfected WWOX with wild type or active form merlin induced growth arrest in cell cycle were greater than single-tranfected merlin or WWOX. However, inactive form merlin and WWOX co-tranfection caused a growth permissive state was identified by flow cytometry analysis. Using soft agarose colony survival assay, we determined that colony number in co-transfected cells with WWOX plus wild type and active form merlin were more doubled than cells transfected with inactive form merlin plus WWOX. On the contrary, co-transfected cells with wild type and active form merlin plus WWOX, colony size was smaller than co-transfected with inactive form merlin plus WWOX. Given the presence of EMT marker proteins in post-irradiated GBM cell lines, we hypothesize that merlin interacts with WWOX may interfere TGF-β signal pathway and EMT. Analysis data from 1306-MG cells after 12 Gy irradiation and transfected WWOX plus individual types of merlin, we found EMT makers such as vimentin and fibronectin were also significantly increased, especially in cells co-transfected with WWOX and inactive form merlin. Data obtained from cells treated with TβRI/II inhibitor suggested that these changes were TGF-β-dependant. According to these results, we conclude that TGF-β signaling recruits merlin/WWOX and via its interaction to promote cell growth and EMT in post-irradiated GBM cell line in vitro. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 567. doi:10.1158/1538-7445.AM2011-567
Does the length of specimen storage affect influenza testing results by real-time reverse transcription-polymerase chain reaction? An analysis of influenza surveillance specimens, 2008 to 2010
Authors: D L Caselton, G Arunga, G Emukule, P Muthoka, L Mayieka, A Kosgey, R Ochola, L W Waiboci, D R Feikin, J A Mott, R F Breiman, M A Katz
Journal: Eurosurveillance (2014)

Binary file ES_Abstracts_Final_ECDC.txt matches
PTU-099 Health-related quality of life in Greek patients with inflammatory bowel disease
Authors: K Argyriou, E Tsakiridou, K Oikonomou, A Kapsoritakis, S Potamianos
Journal: Gut (2012)

<h3>Introduction</h3> Over the last 20 years, health related quality of life (HRQoL) has developed into a scientific index of subjective health status in the management of ulcerative colitis (UC) and Crohn9s disease (CD), with the majority of the available data to come from Northern Europe. But limited data are available from Southern Europe. Aim of our study was to characterise the HRQoL in patients with IBD from Central Greece and the investigation of sociodemographic factors and disease characteristics that affect their HRQoL in order to create a bank of data for future comparative studies. <h3>Methods</h3> The population of the study consisted of 69 IBD patients who were recruited from the IBD referral centre for Central Greece. Data collection included the usage of the Greek version of IBDQ and a card with the sociodemographic and clinical characteristics of the population. Disease activity was assessed by Harvey-Bradshaw activity index for CD and by Simple Colitis Activity Index for UC. The impact of each characteristic in HRQoL was studied with one and two way ANOVA. <h3>Results</h3> 43% of the patients were suffering from UC and 57% from CD. The majority were male (50.7%) but a prevalence of women was noticed in the CD group. 80% of patients were &lt;60 yrs. 60% of the patients with UC and 22.8% with CD were in remission. No significant HRQoL differences were found between UC and CD patients. But, there was a tendency of the CD patients to have higher IBDQ scores and better emotional functioning. ANOVA analysis identified disease activity and symptom9s to explain variations in HRQoL. No significant impact found for sex, educational level, employment and marital status. In contrast, young age (20–40 years) in the CD group had negative impact on their social functioning. <h3>Conclusion</h3> HRQoL did not differ significantly between patients with CD and UC But, there was a tendency of the CD patients to score higher in the IBDQ compared to the UC group which can be justified by the beneficial effect of biologic agents in the management of CD. Young age in CD patients had negative impact on their social functioning which indicates the need for developing supportive networks similar to those of Northern Europe in the South. Finally, disease activity and symptom9s severity were the only factors that affect HRQoL in our population. <h3>Competing interests</h3> None declared. <h3>References</h3> 1. <b>Bernklev</b>, <i>et al.</i> Course of disease, drug treatment and HRQoL in patients with inflammatory bowel disease 5 years after initial diagnosis. <i>Eur J Gastroenterol Hepatol</i> 2005;<b>17</b>:1037–45. 2. <b>Rubin GP</b>, <i>et al.</i> Quality of life in patients with established IBD: A UK general practice survey. <i>Aliment Pharmacol Ther</i> 2004;<b>19</b>:529−53. 3. <b>Canavan C</b>, Abrams KR, Hawthorne B, <i>et al.</i> Long-term prognosis in Crohn9s disease: factors that affect quality of life. <i>Aliment Pharmacol Ther</i> 2006;<b>23</b>:377–85.
P1201 UTILITY OF SERUM MARKERS AND TRANSIENT ELASTOGRAPHY TO IDENTIFY SERIOUS ADVERSE EFFECTS DURING ANTIVIRAL THERAPY IN HCV INFECTED CIRRHOTIC PATIENTS. A MULTICENTER STUDY
Authors: M. Puigvehí, M.C. Londoño, R.M. Morillas, M. Miquel, A. Gallego, S. Lens, A. Baiges, M. Vergara, X. Torras, R. Planas, R. Solà, J.A. Carrión
Journal: Journal of Hepatology (2014)
Abstract 3593: GABA counteracts ethanol induced stimulation of pancreatic cancer cells and normal pancreatic duct epithelia .
Authors: Mohammed H. Al-Wadei, Hussein A.N. Al-Wadei, Hildegard M. Schuller
Journal: Cancer Research (2013)

Abstract Pancreatic cancer has a high mortality rate and alcoholism is a risk factor independent of smoking. We have shown that nicotinic acetylcholine receptors (nAChRs) regulate pancreatic ductal epithelia and pancreatic ductal adenocarcinoma (PDAC) cells in an autocrine fashion by stimulating their production of the stress neurotransmitters noradrenaline and adrenaline that signal through beta-adrenergic receptors (β-ARs). Our current study has investigated the modulation of this autocrine regulatory loop by chronic ethanol and explored the potential prevention of these effects by γ-amino butyric acid (GABA). Treatment for seven days with ethanol at a concentration equivalent to the legal blood alcohol limit in the United States induced protein expression and sensitivity of nAChRs α3, α5 and α7 resulting in increased production of noradrenaline and adrenaline which drive proliferation and migration via cAMP-dependent signaling downstream of β-ARs. Treatment with GABA prevented all of these responses to chronic ethanol, reducing cell proliferation and migration below base levels in untreated cells. Our findings suggest that alcoholism induces multiple cAMP-dependent PDAC stimulating signaling pathways by up-regulating the protein expression and sensitivity of nAChRs that regulate stress neurotransmitters production. Moreover, our data identify GABA as a promising agent for the prevention of PDAC in individuals at risk due to chronic alcohol consumption. Supported by R01CA042829 and R01CA130888 with the National Cancer Institute Citation Format: Mohammed H. Al-Wadei, Hussein A.N. Al-Wadei, Hildegard M. Schuller. GABA counteracts ethanol induced stimulation of pancreatic cancer cells and normal pancreatic duct epithelia . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3593. doi:10.1158/1538-7445.AM2013-3593
Abstract 604: Nek2 is a promising target for triple negative breast cancer treatment strategies
Authors: Jaehyung Lee, Lauren Gollahon
Journal: Cancer Research (2011)

Abstract Although the anticancer drugs paclitaxel and doxorubicin are commonly used to treat many types of solid tumors, their effectiveness is highly variable due to tumor cell resistance. Therefore, it is important to find mechanisms that can be targeted to increase the sensitivity of cancer cells to antitumor agents such as paclitaxel and doxorubicin. NIMA related kinase 2 (Nek2), a serine/threonine kinase is emerging as an important oncogene because of its regulatory role in mammalian cell mitosis. Thus, regulation of the Nek2 expression levels may prove important as a target for cancer treatment. In this study, we investigated whether drug sensitivity was increased in the triple negative breast cancer cell lines MDA-MB-231 and MDA-MB-468 by using antisense oligonucleotides (ASOs) and siRNA against Nek2. MDA-MB-231 and MDA-MB-468 breast cancer cells transfected with Nek2 siRNA or ASO were exposed to various concentrations of paclitaxel and doxorubicin. Cell viability, cell cycle distribution, and apoptosis were evaluated. We observed that drug susceptibility in these transfected cells was dramatically increased compared with either agent alone. Interestingly, the cell proliferation of the Nek2 ASO transfected breast cancer cells was much lower than the Nek2 siRNA transfected breast cancer cells. Our FACS data showed paclitaxel treated cells transfected with ASOs arrested in G2/M phase as expected due to Nek2 regulatory function in centrosome duplication. We observed that ASOs and siRNA against Nek2 work with paclitaxel and by arresting cells at G2/M and progressing the cells to apoptosis. Our results suggest that these drugs in combination with Nek2 siRNA or ASO treatment greatly improve the sensitivity of cancer cells. Thus, these results provide a possible new strategy for the treatment of breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 604. doi:10.1158/1538-7445.AM2011-604
P88 Thalidomide as treatment for IPF associated cough
Authors: G. Saini, T. McKeever, S. Johnson, G. Jenkins
Journal: Thorax (2011)

<h3>Introduction and Objective</h3> Idiopathic pulmonary fibrosis (IPF) is a chronic, irreversible fibrotic disease with more than 5000 incident cases annually in UK. IPF related cough is present in 80% cases and is often refractory to current treatments. Cough has a significant impact on quality of life, causing sleep disturbance, difficulties at work and stress incontinence. A previously published prospective open label phase II trial of thalidomide for treatment of pulmonary fibrosis suggested that IPF associated cough responded well to thalidomide. We have been using thalidomideas an “off-license” indication for selected IPF patients. The objective of this study was to review our experience of using Thalidomide as treatment for cough in IPF. <h3>Methods</h3> Nine patients were referred to Nottingham Academic Interstitial Lung Disease clinic between 2009 and 2011 for assessment of their cough. A modified version of Leicester Cough Questionnaire was used, in conjunction with subjective symptoms, to clinically assess their cough. A trial of PPI (omeprazole 40 mg) and Prednisolone (10 mg) for 6 weeks was given to all subjects. Two were excluded as did not have significant cough, one patient declined thalidomide after initial screening. <h3>Results</h3> Six patients were treated with thalidomide. Four had IPF, one had Hypersensitivity Pneumonitis and one had fibrotic Cryptogenic Organising Pneumonia. 72% were males with mean age 69 years (range 51–88 years). The median pre-thalidomide cough score was 74.5 (IQR 13.25) and post treatment cough score was 51.5 (IQR 49.25). This was statistically significant (p=0.046). Three stopped thalidomide subsequent to rash. Two patients are currently stable with 50 mg once daily, and 1 with 50 mg alternate daily of thalidomide. <h3>Conclusion</h3> Our observation of a carefully selected cohort of patients suggests that thalidomide has potential for treatment of IPF associated cough. It works quickly (within days) as reflected both subjectively and objectively via questionnaire, even when prednisolone has failed. However, it does have a significant side-effect profile. Identification of thalidomide9s mechanism of action may aid the development of novel, effective anti-tussive therapy for this debilitating aspect of IPF. We will assess this in randomised open label trial comparing thalidomide vs prednisolone for treatment of IPF associated cough.
AB1183-HPR Physical Activity in Patients with Systemic Sclerosis and Matched Controls
Authors: C. Boström, A. Åkerström, L. Björnådal, H. Alexanderson, E. Svenungsson, A. Nordin
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Previous studies have demonstrated that physical activity has several benefits among the general population. In patients with systemic sclerosis (SSc) few studies concerning the frequency of physical activity are sparse and no studies have compared different aspects of physical activity between patients with SSc and the general population. <h3>Objectives</h3> The aim of the study was to examine different aspects of self-reported physical activity in patients with SSc and matched population controls. <h3>Methods</h3> 106 patients, fulfilling the ACR criteria for SSc, and 106 controls, individually matched for age, gender and living region were investigated clinically by a rheumatologist. All participants filled out a questionnaire about exercise capacity, physical activity and sedentary behaviour. A nurse collected the questionnaire and measured weight and height. <h3>Results</h3> In total, 178 women and 34 men were included. The mean age of the patients was 61.9 (SD 12.4) and for controls 62.01 (SD 12.4) years. The Body Mass Index was lower in patients, mean 24.4 (SD 3.7), than controls, 26.1 (3.9) (p=0.001). The patients reported lower capacity for walking, jogging and running compared to controls (p&lt;0.001). There were no significant difference in physical exercise the previous year between the patients and the controls (p=0.1). Similar results concerning physical activity on "low to moderate" intensity 6-7 times/week were reported in patients (36%) and controls (31%) (ns). Concerning physical activity on "high" intensity 4-7 times/week the results also were similar in patients (17%) and controls (19%) (ns). <h3>Conclusions</h3> This is the first study reporting different aspects of physical activity in patients with SSc compared to matched controls. Although the patients reported lower exercise capacity than controls, this study indicated that there was no difference in physical exercise the previous year. Only approximately 1/5 of both patients and controls reached the general recommendations for physical activity, i.e. on high intensity. Future interventions should focus on supporting increased physical activity and exercise in patients with SSc. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5299
Cyclodextrin promotes atherosclerosis regression via macrophage reprogramming
Authors: Sebastian Zimmer, Alena Grebe, Siril S. Bakke, Niklas Bode, Bente Halvorsen, Thomas Ulas, Mona Skjelland, Dominic De Nardo, Larisa I. Labzin, Anja Kerksiek, Chris Hempel, Michael T. Heneka, Victoria Hawxhurst, Michael L. Fitzgerald, Jonel Trebicka, Ingemar Björkhem, Jan-Åke Gustafsson, Marit Westerterp, Alan R. Tall, Samuel D. Wright, Terje Espevik, Joachim L. Schultze, Georg Nickenig, Dieter Lütjohann, Eicke Latz
Journal: Science Translational Medicine (2016)

The cyclic oligosaccharide 2-hydroxypropyl-β-cyclodextrin facilitates atheroprotective mechanisms through oxysterol-mediated reprogramming of macrophages.
SP0052 Taking action against stigmatization
Authors: A.T. Kennedy
Journal: Annals of the Rheumatic Diseases (2013)

<i>"Taking Action Against Stigmatization"</i> considers the interventions being currently researched and offered to combat both the depression and self-seclusion created by a disfiguring and disabling chronic disease, and the bias and exclusion by society that is often unconscious or based on incorrect facts. Using scleroderma (SSc) as an example, this session will investigate the growing portfolio of psychosocial programs that can significantly improve Quality Of Life (QOL) and return those with the condition to a position in which to participate as happy and useful members of society. The lecture will discuss surveys that have investigated the effects of SSc such as pain, fatigue, body-image, and emotional distress, which have a major influence on daily activities and QOL, and other symptoms such as gastrointestinal issues and sexual difficulties, which are among the worst prevalent and disruptive physical problems socially, before turning to some of the programs developed in order to meet these needs. Self-stigmatization causes significant disruptions in social life, a burden considered by many as the worst consequence of their disease. Depression, low self-esteem, concerns with physical appearance, and uncertainty about future outcomes are symptoms of chronic disease that need treatment as much as kidney, lung, and gut involvement. <h3>Disclosure of Interest</h3> None Declared
Image Analysis in Patients with Cancer Studied with a Combined PET and CT Scanner
Authors: M Charron, T Beyer, N N Bohnen, P E Kinahan, M Dachille, J Jerin, R Nutt, C C Meltzer, V Villemagne, D W Townsend
Journal: Clinical Nuclear Medicine (2000)

To compare combined whole-body PET and CT images of different cancers with PET images alone.Thirty-two patients with known or possible cancers were examined using a combined positron emission tomographic (PET) and computed tomographic (CT) scanner. All data were acquired using this same combined scanner. After an injection of F-18 fluorodeoxyglucose (FDG), noncontrast helical CT imaging of the neck, chest, abdomen, or pelvis was performed. The spiral CT was followed by a PET scan covering the same axial extent as the CT.Coregistered PET-CT images identified and localized 55 lesions. In 10 patients (31%), areas with variable amounts of normal physiologic FDG uptake were distinguished from potential uptake of FDG in a nearby neoplastic lesion. Improved localization was achieved in 9 patients (for a total of 13 lesions, or 24%).Combined PET-CT images appear more effective than PET images alone to localize precisely neoplastic lesions and to distinguish normal variants from juxtaposed neoplastic lesions.
Oocyte cryopreservation: where are we now?
Authors: Catrin E. Argyle, Joyce C. Harper, Melanie C. Davies
Journal: Human Reproduction Update (2016)

Since the first live birth from oocyte cryopreservation three decades ago, oocyte cryopreservation has become an important component of ART. Cryopreservation techniques have evolved, leading to higher success rates and the introduction of oocyte cryopreservation into IVF clinics worldwide. Concurrently, there has been an increase in patient demand, especially for so-called 'social egg freezing' that allows women to preserve their fertility in anticipation of age-related fertility decline. This review addresses a need to evaluate the current status of oocyte cryopreservation. It explores current techniques and success rates, clinical applications, the rise of elective oocyte cryopreservation, and future implications.A search was performed using Web of Science and PubMed databases for publications between January 1980 and December 2015. Keywords used included 'egg freezing', 'oocyte freezing', 'oocyte cryopreservation', 'oocyte vitrification', and 'fertility preservation'.The success rate of oocyte cryopreservation has risen, and the increasing use of vitrification offers has improved outcomes, with IVF pregnancy rates now similar to those achieved with fresh oocytes. There are conflicting opinions about the comparative success rates of open and closed vitrification. Patients are accessing and receiving oocyte cryopreservation for a wide range of indications, and there has been a marked increase in patient numbers and oocyte cryopreservation cycles. Oocyte cryopreservation for circumventing age-related infertility is becoming more widely accepted.Oocyte cryopreservation is an established component of ART, with vitrification now being the cryopreservation technique of choice. Increasing numbers of women undergo oocyte cryopreservation for both medical and social reasons. It is important to continue auditing outcomes and reporting long-term follow-up of children born from frozen-thawed oocytes.
Unusual Case of Diagnosis of Pigeon Breeder’s Hypersensitivity Pneumonitis With Ventilation–Perfusion Lung Scan
Authors: H Kolesnikov-Gauthier, M F Borie, B Maitre, J Rosso, A Schaeffer, M Meignan
Journal: Clinical Nuclear Medicine (2000)

The authors describe a patient thought to have pulmonary embolism. Results of a perfusion scan were normal, but Tc-99m DTPA aerosol ventilation lung scan showed, in successive views, decreased activity of the deposited aerosol. The lung Tc-99m DTPA clearance was measured and was markedly increased. This suggested possible hypersensitivity pneumonitis, which was later confirmed as pigeon breeder's disease. The decreased activity observed on the DTPA ventilation scan must be directed toward increased lung clearance and its measurement.