tbuckley/cabot-search huggingface dataset:
Abstract 2161: MLK3 signals to paxillin in chemokine-induced migration and invasion, and is necessary for breast cancer metastasis
Authors: Jian Chen, Kathleen A. Gallo
Journal: Cancer Research (2012)

Abstract Breast cancer metastasis is the major cause for mortality of patients with invasive breast cancer. Metastasis involves multiple steps including cancer cell invasion through basement membrane, intravasation, survival in the circulation, extravasation and colonization of distant organs. Identification of the signaling pathways through which prometastatic factors promote cancer cell migration and invasion is key to develop effective therapeutics for breast cancer. Mixed lineage kinase 3 (MLK3) is a MAP3K involved in activating each of the three major MAPK signaling pathways. The MLK3-JNK-AP-1 signaling axis has been shown to be required in breast cancer cell migration and invasion. Herein, we demonstrate that the prometastatic factors, CXCL12 and HGF, signal through MLK3-JNK to induce phosphorylation of the focal adhesion scaffold, paxillin on Ser 178, which is required for migration and invasion of human breast cancer cells. Furthermore, we show that MLK3 regulates the phosphorylation of paxillin at Tyr 118 and promotes the interaction of FAK with paxillin, which can be blocked by inhibition of MLK3. To further understand the role of paxillin phosphorylation at S178, a nonphosphorylatable paxillin mutant S178 A was generated. Expression of S178A blocks Y118 phosphorylation of paxillin in breast cancer cells. Silencing of mlk3, inhibition of JNK, or expression of the nonphosphorylatable paxillin S178A increases focal adhesion numbers and increases Rho activity, suggesting that the MLK3-JNK-paxillin axis limits Rho activity in breast cancer cells. Furthermore, MLK3 silencing is sufficient to prevent formation of metastases in a mouse xenograft model of spontaneous breast cancer metastasis. Our findings have identified a novel role of MLK3 in regulation of focal adhesion dynamics and cytoskeletal remodeling in the process of breast cancer cell migration and suggest that MLK3 may be a potential therapeutic target for metastatic breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2161. doi:1538-7445.AM2012-2161
Abstract 2793: Folate, B vitamin and methionine intake and risk of colon cancer in relation to <i>BRAF</i> mutation and CpG Island Methylator Phenotype (CIMP)
Authors: Eva S. Schernhammer, Katsuhiko Nosho, Edward L. Giovannucci, Charles S. Fuchs, Shuji Ogino
Journal: Cancer Research (2010)

Abstract BACKGROUND AND AIMS: One-carbon metabolism appears to play an important role in DNA methylation reaction. Evidence suggests that a low intake of folate or other B vitamins or high alcohol consumption increases colorectal cancer risk. How dietary folate and B vitamins affect the CpG island methylator phenotype (CIMP) and BRAF mutation status in colon cancer remains uncertain. METHODS: Utilizing 472 colon cancer tissue specimens from a large prospective cohort of women in the Nurses’ Health Study, we sequenced BRAF codon 600 and quantified DNA methylation in eight CpG islands [CACNA1G, CDKN2A (p16), CRABP1, IGF2, MLH1, NEUROG1, RUNX3, and SOCS1], which have been validated as a CIMP marker panel. In addition, we examined 8 other CpG islands (CHFR, HIC1, IGFBP3, MGMT, MINT-1, MINT-31, p14, WRN). We examined the relationship between intake of folate and other one-carbon nutrients and colon cancer risk, according to BRAF mutation or CIMP status. RESULTS: We were able to sequence BRAF in 472 incident colon cancers, and determine CIMP status in 462 tumors. Compared with women reporting intake of &amp;lt;200μg of folate per day, the multivariate relative risk (RR) of developing CIMP-low/0 tumors (with &amp;lt;6/8 methylated CIMP-specific markers) was 0.73 (95% confidence interval (CI), 0.53-1.02) for those who took ≥400μg of folate per day. In contrast, total folate intake had no influence on risk for CIMP-high tumors (with ≥6/8 methylated CIMP-specific markers) [multivariate RR, 0.98; 95% CI, 0.54-1.77, comparing ≥400 to &amp;lt;200μg of folate per day]. Vitamin B6 intake did not appear to differentially influence risks for CIMP-high and CIMP-low/0 tumors (Pheterogeneity =0.63). In contrast, a higher methionine intake appeared to lower the risk of CIMP-high cancers (top versus bottom quintile, multivariate RR, 0.68; 95% CI, 0.35-1.31) whereas it had no effect on CIMP-low/0 tumors (multivariate RR, 1.04; 95% CI, 0.71-1.52; Pheterogeneity =0.007). Using the 16-marker CIMP panel did not substantially alter our results. Folate, B vitamin or methionine intake did not differentially affect colon cancer risk by BRAF status. CONCLUSIONS: Lower levels of folate and vitamin B6 intake were associated with increased risk of CIMP-low/0 colon tumors, whereas higher levels of methionine intake appeared to lower the risk of CIMP-high tumors. These nutrients did not appear to affect BRAF mutation status. Our data support epigenetic alterations as potential mechanisms through which one-carbon nutrients exert their influence on colon carcinogenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2793.
Abstract 3469: EphrinB2 promotes endothelial cell survival and vascular integrity
Authors: Ombretta Salvucci, Dragan Maric, Fan Zhang, Xuri Li, Mark Basik, Marguerite Buchanan, Giovanna Tosato
Journal: Cancer Research (2011)

Abstract EphrinB transmembrane ligands and their cognate EphB receptors critically contribute to vascular development through cell-to-cell signaling by the EphB receptor (forward signaling) and by the EphrinB ligand (reverse signaling). Previous studies demonstrated that mutant mice lacking ephrinB2 or EphB4 and a proportion of mice lacking EphB2 and EphB3 display severe defects in the embryonic vascular system and die at the embryo stage likely due to these vascular defects. Previously, we showed that EphB2, EphB4 and EphrinB2 are required for mature endothelial cell assembly into capillary structures. In the currently studies, we observed that silencing EphrinB2 expression in different types of primary human endothelial cells causes significant and time-dependent apoptotic cell death, as measured by cleavage of Poly(ADP-ribose) Polymerase (PARP), caspase-3 and caspase-8. VEGF, bFGF and SDF-1 variously modulated apoptosis induced by loss of EphrinB2 expression in endothelial cells. Gene expression profiling revealed that EphrinB2 silencing is associated with reduced expression of genes linked to regulation of the cell cycle progression and cytoskeleton organization, and with increased expression of pro-apoptotic genes. Breast cancer tissue from a patient treated with bevacizumab, a humanized monoclonal antibody that neutralizes VEGF, displayed evidence of endothelial cell apoptosis, which was associated with selectively reduced EphrinB2 expression, suggesting a link between VEGF signaling, EphrinB2 expression and cell death. These studies show that EphrinB2 expression is critical to endothelial cell survival in vitro and suggest that EphrinB2 contributes to blood vessel integrity in vivo. Thus, in addition to its well-characterized function as a regulator of cell-to-cell adhesion and repulsion, EphrinB2 is an endothelial pro-survival molecule, which may serve as a novel target for anti-angiogenic therapies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3469. doi:10.1158/1538-7445.AM2011-3469
Congress Considers Eliminating Nearly All Tobacco Advertising
Authors: T. Hensley
Journal: JNCI Journal of the National Cancer Institute (1989)

Congress Considers Eliminating Nearly All Tobacco Advertising Tim Hensley Tim Hensley Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 81, Issue 17, 6 September 1989, Pages 1281–1282, https://doi.org/10.1093/jnci/81.17.1281 Published: 06 September 1989
Abstract LB-370: Radiation and hypoxia-induced bystander effect in human lung cancer cells
Authors: Slavisa Tubin, Seema Gupta, Mansoor M. Ahmed
Journal: Cancer Research (2011)

Abstract Purpose: The bystander effects of radiation are well documented. However, it is shown that many cell lines that respire anaerobically do not show cytotoxic bystander effects. There is no existing data on the acute hypoxia-induced bystander effects. The purpose of this study was to investigate the status of radiation-induced bystander effect in normoxic, hypoxic and normoxic-hypoxic mixed conditions that are equivalent to in vivo tumor compartments as well as acute hypoxia-induced bystander effect. Methods: Two lung cancer cell lines, A549 and H460 were used. The cells were exposed either to real hypoxia (&amp;lt; 2 % oxygen) for 3 days or incubated in normoxic conditions and then irradiated (2 or 10Gy) after changing the media. After 24h (irradiated and unirradiated cells were continued to be in the hypoxic or normoxic environment respectively), unirradiated hypoxic (H-CM) or normoxic (N-CM) conditioned media and irradiated hypoxic (H-RCM) or normoxic (N-RCM) conditioned media was collected and cells were incubated with fresh media for another 24h before collecting the second respective CM. Unirradiated normoxic cells were exposed to H-CM, N-CM, H-RCM or N-RCM and cell growth was monitored dynamically in real time using real time cell electronic sensing system (RT-CES). Results: Our preliminary results in A549 cells showed that H-RCM was much more effective in inducing growth delay compared to N-RCM. For all the treatments except for 2Gy N-RCM, which promoted slight cellular growth, RCM induced significant growth delay compared to CM. Further, as expected N-RCM obtained after 10Gy irradiation was more cytotoxic/cytostatic than 2Gy N-RCM. Interestingly, H-RCM obtained after 2Gy and 10Gy irradiation did not show significant differences in the growth delay of A549 cells. During the first 48h under real hypoxic conditions (24 or 48h H-CM), the H-CM promoted slight increase in cellular growth followed by significant growth delay which were most pronounced at 96h. When, in addition to the bystander factors, the cells were exposed to 2Gy direct irradiation, the growth was severely affected. Currently, we are performing these experiments in more radiosensitive cell line, H460 and also investigating the effects of acute hypoxia and mixed normoxia-hypoxia on generation of bystander effects. Conclusions: The findings from this study demonstrate for the first time that irradiation of hypoxic cells and exposing the cells to acute hypoxia lead to significant bystander effect that has important clinical implications. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-370. doi:10.1158/1538-7445.AM2011-LB-370
IMPACT OF LESION CORONARY CALCIUM SCORE ON OUTCOMES FOLLOWING EVEROLIMUS-ELUTING STENT IMPLANTATION
Authors: Tatsuyuki Sato, Hiroshi Kadowaki, Nozomi Fuse, Taishi Okuno, Yu Sato, Kentaro Yasuhara, Furui Koichi, Nahoko Kato, Yu Horiuchi, Toshio Kinoshita, Satoru Kishi, Shuzou Tanimoto, Jiro Aoki, Kazuhiro Hara, Kengo Tanabe
Journal: Journal of the American College of Cardiology (2016)
Warthin's Tumor Demonstrated With Tc-99m Pertechnetate SPECT and CT
Authors: MARY KAY PETERSON, ROBERT R. BUTLER
Journal: Clinical Nuclear Medicine (1998)

A 55-year-old woman presented with a four-month history of bilateral preauricular swelling. She denied pain, fever, or symptoms of upper respiratory infection. Physical exam revealed bilateral parotid gland enlargement, without inflammatory changes. A contrasted CT demonstrated bilateral parotid hyperdense masses, the largest measuring 3 cm on the right, and a smaller 1.5 cm mass on the left. A planar Tc99m pertechnetate salivary scan demonstrated increased and delayed uptake in the inferior pole of the right parotid, consistent with a Warthin's tumor. Delayed uptake in the left parotid mass was not well defined on planar imaging. Further evaluation with SPECT demonstrated delayed uptake in both parotid lesions. The findings of bilateral Warthin's tumors was confirmed by surgical excision of both lesions.
P62Role of microRNAs associated with non-alcoholic fatty liver disease in sudden cardiac death from coronary artery disease
Authors: A Braza-Boils, J Mari-Alexandre, P Molina, D Domingo, Y Abellan, J Sancho, MA Arnau, J Giner, A Estelles, E Zorio
Journal: Cardiovascular Research (2014)

Purpose: Coronary artery disease (CAD) is the leading cause of sudden cardiac death (SCD). It has recently been pointed to non-alcoholic fatty liver disease (NAFLD) as a new risk factor for the development of CAD. The possible mechanisms linking NAFLD and CAD include inflammation, dyslipidaemia, insulin resistance, and direct impact of NAFLD on coronary arteries. microRNAs (miRNAs) are 21–22 nucleotide non-coding RNAs that regulate gene expression and play fundamental roles in several diseases. And they have been related to the cholesterol homeostasis, therefore they could contribute to the development of NAFLD. The aim of this study is 1) to evaluate the relationship between NAFLD and SCD from severe CAD in forensic autopsies and 2) to quantify the expression of several liver miRNAs related to lipid metabolism and NAFLD and to correlate the expression levels of these liver miRNAs with the presence of steatohepatitis (NASH), obesity and lipid profile. Methods: 133 cases of SCD due to CAD (CAD-SCD) and 106 cases of non-CAD sudden death (control group, non-CAD-SD) were prospectively included from 2008 to 2013 following a broad protocol created by our study group to offer a multidisciplinary approach of SCD. miRNAs -122-5p, -33a-5p, -34a-3p, -21-5p, -29c-3p, -27a-3p and -106b-5p were quantified by qRT-PCR in frozen liver tissues. Forensic investigation included compilation of circumstances of death and premortem clinical information, autopsy examination including body mass index (BMI) and abdominal circumference (AC). Results: In both groups a male predominance was observed. The presence of NAFLD was significantly higher in the CAD-SCD group than in the group of non-CAD-SD (62% vs 26%, p<0.001), with more advanced forms of the disease (NASH). In both groups, the presence of hepatic disorder correlated with BMI and AC (p<0.01). A significant increase of miR-34a-3p (p<0.05) and a significant decrease in miR-122-5p and -29c-3p (p<0.01) in the CAD-SCD with NASH group versus non-CAD-SD without NAFLD group were observed. A significant inverse correlation (p<0.05) between miRNAs -29c-3p and -122-5p and AC or BMI were obtained. Moreover, we found a significant inverse correlation (p<0.05) between miR-122-5p and LDL/HDL ratio; and a significant direct correlation (p<0.05) between miR-34a and GGT was observed. Conclusions: The CAD-SCD is associated with NAFLD and NASH. The miRNAs studied appear to be associated with NAFLD severity.
Open Microsurgical Autograft of Adrenal Medulla to the Right Caudate Nucleus in Two Patients with Intractable Parkinson's Disease
Authors: Ignacio Madrazo, René Drucker-Colín, Víctor Díaz, Juan Martínez-Mata, César Torres, Juan José Becerril
Journal: New England Journal of Medicine (1987)

Recent experimental studies and one clinical case have suggested that grafting tissue from the adrenal medulla into the brain may ameliorate the signs of Parkinson's disease. We describe the treatment of two young patients (35 and 39 years old) with intractable and incapacitating Parkinson's disease, in whom fragments of the adrenal medulla were autotransplanted to the right caudate nucleus. Clinical improvement was noted in both patients at 15 and 6 days (respectively) after implantation and has continued in both. Rigidity and akinesia had virtually disappeared in the first patient at 10 months after surgery, and his tremor was greatly reduced. A similar degree of improvement was present in the second patient at three months. We conclude that autografting of the adrenal medulla to the right caudate nucleus was associated with a marked improvement in the signs of Parkinson's disease in two patients, but our results are preliminary and further work is necessary to see whether this procedure will be applicable over the long term in other types of patients with Parkinson's disease. (N Engl J Med 1987; 316: 831–4.)
ASSOCIATION OF POST-NORWOOD ARRHYTHMIA WITH SUBSEQUENT OUTCOMES IN THE SINGLE VENTRICLE RECONSTRUCTION TRIAL
Authors: Matthew Oster, Shan Chen, Eric Gerstenberger, Yaniv Bar-Cohen, Matthew Brothers, Nicole Cain, Steven Colan, Richard Czosek, Jamie Decker, David Gamboa, Salim Idriss, Joel Kirsh, Martin LaPage, Richard Ohye, Elizabeth Radojewski, Maully Shah, Eric Silver, Anoop Singh, Joel Temple, John Triedman, Jonathan Kaltman
Journal: Journal of the American College of Cardiology (2015)
Abstract 2656: Association of daunorubicin to a lipidic nanoemulsion (NEM-ODNR) - in vivo tumor growth inhibition
Authors: Thais C. Contente, Iara F. Kretzer, Durvanei A. Maria, Tathiana Capeto, Ricardo Fock, Raul C. Maranhao
Journal: Cancer Research (2010)

Abstract After injection into blood stream an artificial nanoemulsion (NEm) that mimics LDL composition binds to LDL receptors and concentrates in tumor tissues that show LDL receptor overexpression. NEm has been used as carrier to target drugs against neoplastic tissues and was shown to diminish toxicity while increase tumoral inhibition growth in murine models, showing more effective and selectiveness. Daunorrubicin (DNR) is an anthracycline antibiotic that possesses broad spectrum antineoplastic activity, and is one of the most important anticancer agents in use. However, clinical utility is hampered by cumulative, dose limiting cardiotoxicity, myelosupression, and the development of drug resistence. Derivative form of daunorubicin oleate (ODNR) was synthesized and associated to NEM by prolonged ultrassonication and ultracentrifugation Antitumor activity of NEM-ODNR were tested in B16F10 melanoma bearing mice and compared with commercial formulation (DNR) and control group (saline). Tumor growth, presence of metastatic nodes and hematological profile were evaluated. The cytotoxicity of NEm-ODNR was diminished by five times compared to DNR. In dose acute toxicity studies the NEm-ODNR tolerability was 72-times higher compared to commercial DNR. The tumor growth inhibition rate for NEM-ODNR was over 35% when compared to DNR and 59% compared to saline (p &amp;lt; 0,001). There was a significant reduction in animals bearing metastasis for NEM associated treated group (30%) in comparison to the commercial treated (80%). In control group all animals developed metastatic tumors. Hematological profile showed that NEM-ODNR treatment had a 15% raise for red blood cells, compared to 29% of reduction for DNR, 25% of reduction to white blood cells compared to 55% for DNR and 45% reduction of platelets compared to 57% for DNR. Flow cytometry suggested greater antitumor activity for NEm-ODNR group showing remarkable arrest (47%) on DNA fragmentation phase of cell cycle compared to 26% to DNR. Those tests are essential for evaluating the NEM-ODNR formulation and its implementation of future clinical trials. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2656.
COST-EFFECTIVENESS COMPARISON OF INTRA AORTIC BALLOON PUMP VERSUS LEFT VENTRICULAR ASSIST DEVICES AS BRIDGE TO HEART TRANSPLANT STRATEGIES
Authors: Javier Amione-Guerra, Kelli J. Elizondo, Laurie Loza, Arvind Bhimaraj, Barry Trachtenberg, Guha Ashrith, Myung Park, David Bernard, Jerry Estep
Journal: Journal of the American College of Cardiology (2016)
Detection of Late Chest Wall Recurrence of Breast Carcinoma During Tc-99m Sestamibi Parathyroid Scintigraphy
Authors: Gary R Conrad, David A Sloan, Partha Sinha
Journal: Clinical Nuclear Medicine (2003)

From the Nuclear Medicine Section, Department of Radiology,* and the Department of Surgery,† University of Kentucky Chandler Medical Center, Lexington, Kentucky Received for publication July 17, 2002. Accepted October 18, 2002. Reprint requests: Gary R. Conrad, Department of Radiology, HX-311, 800 Rose Street, Lexington, Kentucky 40536-0293. E-mail: [email protected]
Diagnostic flexible laparoscopy: A single incision procedure
Authors: L Polese, S Merigliano
Journal: Endoscopy (2012)

Objective of the study: Diagnostic laparoscopy is a minimally invasive method which enables the direct inspection of intra-abdominal organs. Simple additional procedures, such as histological or cytological sampling, fluid aspiration and intraperitoneal lavage can be carried out by positioning additional trocars.
Who was James Lind, and what exactly did he achieve
Authors: Jeremy Hugh Baron
Journal: Journal of the Royal Society of Medicine (2013)

Iain Milne considered the possibility that Lind's report of a controlled trial may be a fabrication1 and kindly cited my review.2 However the credit should instead go to Graham Sutton who knew, as I did not, that the ship's papers of HMS Salisbury survived and there were no sailors sick with scurvy on this ship in May 1747.3
ACUTE CORONARY SYNDROME CAUSED BY NON-CRITICAL CORONARY STENOSIS: INSIGHT FROM AN IN VIVO OPTICAL COHERENCE TOMOGRAPHY STUDY
Authors: Haibo Jia, Sining Hu, Amir Lerman, Catalin Toma, Harold Dauerman, Daniel Ong, Rocco Vergallo, Jinwei Tian, Tsunenari Soeda, Yoshiyasu Minami, Lei Gao, Hang Lee, Shaosong Zhang, Jingbo Hou, Bo Yu, Ik-Kyung Jang
Journal: Journal of the American College of Cardiology (2015)