tbuckley/cabot-search huggingface dataset:
Abstract 3959: Growth suppression by TGF-β in responsive colon carcinoma is opposed by miR-20a through mechanisms altering p21WAF1 up-regulation
Authors: Viktorija Sokolova, Antonio Fiorino, James F. Reid, Elisabetta Crippa, Marco A. Pierotti, Manuela Gariboldi
Journal: Cancer Research (2011)

Abstract Loss of response to TGF-β occurs in many cancers and disruption of its regulatory circuitry appears as a central event in the genesis of colorectal cancer (CRC) malignancy. Lack of inhibitory response to TGF-β is common to most colon cancer cell lines. However, inactivating mutations at receptors and transducers occur in less than a half of neoplastic colon tissues, which underscore the significance of additional mechanisms diverting TGF-β growth suppression. In this context, abrogation of TGF-β response by some miRNAs has been recently reported. By searching for miRNAs in regions showing copy number changes and concordant gene expression in 36 sporadic CRCs compared to their normal counterpart, we identified the miR-17-92 cluster on the 13q31 locus, which is gained and highly expressed at early stages of CRC. MiR-20a from miR-17-92 cluster is a paralog of the miR-106b, which affects TGF-β sensitivity in gastric carcinoma. We hypothesized an involvement of miR-20a in the suppression of TGF-β response in CRCs and selected the TGF-β sensitive FET colon carcinoma, expressing low miR-20a levels, to investigate the relationship between enhanced expression of miR-20a and TGF-β sensitivity and address growth inhibition. We report that miR-20a affects regulation of p21WAF1 expression, has a negative and significant effect on the cytostatic response mediated by TGF-β – evaluated by BrdU incorporation, MTT assay, and cell-cycle analysis – but shows little effect on TGF-β untreated cells. Although p21WAF1 transcript and protein are significantly decreased in cells treated or not with the cytokine, we could observe that the p21 up-regulation driven by TGF-β is strongly subverted by miR-20a. One potential mechanism of p21 down-modulation by miR-20a can be the targeting of its 3’UTR, as occurring for miR-106a. Moreover, we could observe that miR-20a is also able to block the transactivation of the 2.3-kb p21 promoter upon TGF-β stimulation, as assessed by luciferase-based assay, but not the activation of the Smad3/4-reponsive reporter. Down-modulation of c-Myc by TGF-β, crucial to regulation of p21 transcription, is also subverted by miR-20a delivery into cells. Our experiments suggest for miR-20a an interference with the TGF-β homeostasis in colon addressing the up-regulation of p21 expression, through mechanisms involving more effectors of the TGF-β cascade. Overall, miR-20a seems to participate in the abrogation of this key regulatory response in colonic epithelium. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3959. doi:10.1158/1538-7445.AM2011-3959
Anastomose gastro-jéjunale par technique 'N.O.T.E.S' exclusive sur cochon vivant: étude de faisabilité et d'efficacité
Authors: G Vanbiervliet, JM Gonzalez, E Garnier, S Giusiano, MC Saint Paul, S Berdah, M Barthet
Journal: Endoscopy (2012)

Les techniques endoscopiques d'accès péritonéal par voie trans digestive naturelle (ou N.OT.E.S) permettraient de réduire la morbi mortalité de certains gestes chirurgicaux. L'anastomose gastro jéjunale pour sténose digestive ou dans l'optique d'une chirurgie bariatrique a démontré son utilité mais présente des complications potentiellement sévères. Le but de cette étude expérimentale est de déterminer la faisabilité d'un modèle simple d'anastomose gastro jéjunale in vivo chez le cochon en technique N.O.T.E.S exclusive.
AB0747 Assessment of cortical discontinuities in interphalangeal joints with hrpqct in comparison with radiography and microct imaging.
Authors: J. Williams, B. van Rietbergen, C. Arts, J. van den Bergh, A. van Tubergen, A. Scharmga, P. Geusens
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Bone erosions scored on classical 2D radiographs (CR) are a hallmark for diagnosis, disease prediction and evaluation of drug effects in patients with rheumatoid arthritis (RA). However, on a population level, more erosions are found using 3D imaging than with 2D CR.<sup>1</sup> <h3>Objectives</h3> To compare the presence of bone erosions on CR with the presence of cortical breaks using high-resolution quantitative computed tomography (HRpQCT) in cadaver human fingers. <h3>Methods</h3> Five human female cadaveric index fingers (aged 81,4 ± 11 years) were radiographed using CR and scanned with a HRpQCT device as used in vivo (Xtreme CT, Sanco, Swiss, spatial resolution 82 µm) and with µCT (µCT 80, Scanco, Swiss, spatial resolution: 18 µm). For each CT measurement, three regions of interest were measured; an 18 mm area covering the metacarpal phalangeal (MCP) joint and two 9 mm areas covering the proximal interphalangeal (PIP) and the distal interphalangeal (DIP) joints. The CR images were assessed by an experienced rheumatologist for the presence of erosions. In the CT data, cortical bone breaks with a diameter &gt;0.5 mm were manually counted throughout the transversal CT slices of both the HRpQCT and the µCT images. <h3>Results</h3> In total, 15 interphalangeal joints were assessed. Using CR, two bone erosions were found in two joints, whereas in HRpQCT images, 35 cortical breaks with a diameter &gt;0.5 mm were counted in 13 joints (chi square 13.3, p&lt;0.001 versus CR). Using µCT, a total of 46 cortical breaks &gt;0.5 mm wascounted in 14 joints (chi square 16.2, p&lt;0.001 versus CR, no significant difference versus HRpQCT). <h3>Conclusions</h3> This pilot <i>in vitro</i> study in human cadaver finger joints shows that high resolution CT imaging enables to identify significantly more cortical breaks than CR. HRpQCT is therefore a more sensitive technique for the evaluation of cortical breaks in rheumatic diseases in interphalangeal joints. The results indicate that more small individual cortical breaks can be identified with µCT with respect to HRpQCT due to a higher resolution and less signal noise. <h3>References</h3> Stach et al. A&amp;R, Vol. 62, No. 2, February 2010, pp 330–339 <h3>Disclosure of Interest</h3> None Declared
Reversible Severe Lower Limb Pain During a Dipyridamole Sestamibi Myocardial Perfusion Study: A Case Report
Authors: DAVID C. WONG, EDWIN SZETO
Journal: Clinical Nuclear Medicine (1998)

A 68-year-old woman reported acute severe pain in both lower limbs during a dipyridamole sestamibi myocardial perfusion scan. The pain was rapidly relieved by the administration of intravenous aminophylline. A review of the literature on the adverse effects of dipyridamole did not include this symptom.
SAT0098 Seropositivity of anti-cyclic citrullinated peptide antibody is more prevalent in unaffected first-degree relatives with multicases families of rheumatoid arthritis
Authors: W.-T. Chung, H. Lee, J.H. Kim, S.-H. Park, J.-Y. Choe, S.-K. Kim
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> This study elucidates the prevalence of seropositivity and determinants for rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP), and anti-mutated citrullinated vimentin (anti-MCV) antibodies in unaffected first-degree relatives (FDRs) of rheumatoid arthritis (RA) patients. <h3>Methods</h3> The subjects (135 with RA and 202 of their FDR) were enrolled in this case-control study. Serum RF, anti-CCP antibody, and anti-MCV antibody were measured. Subjects with multi-case families (two or more affected FDRs within the study family) were identified. Multivariate logistic regression analysis was used to determine the risk factors associated with RA-related autoantibodies. <h3>Results</h3> Seropositivity for RF, anti-CCP antibody, and anti-MCV antibody in unaffected FDRs was 14.4%, 5.0%, and 13.4%, respectively. Anti-CCP antibody was more prevalent in FDRs with multi-case families (17.8%) than in those without multi-case families (1.3%) (p&lt;0.0001). Significant correlations were detected between RA-associated autoantibodies in the FDR group (<i>r</i> =0.366, p&lt;0.0001 between RF and anti-CCP antibody; <i>r</i> =0.343, p&lt;0.0001 between RF and anti-MCV antibody; <i>r</i> =0.849, p&lt;0.0001 between anti-CCP antibody and anti-MCV antibody). The anti-CCP antibody seropositivity in FDRs was significantly associated with the presence of multi-case families within FDRs (odds ratio: 49.8, 95% confidence interval: 5.6 – 441.6 after adjustment for age and gender). <h3>Conclusions</h3> The association between anti-CCP antibody seropositivity and having a multi-case family in the unaffected FDR group suggests that genetic and/or environmental factors may increase the unaffected FDR subjects' risk of developing RA. <h3>Disclosure of Interest</h3> None Declared
P689Examination of radiation-induced heart damage using in vitro and in vivo animal models
Authors: L Kiscsatari, Z Varga, A Gorbe, N Morvay, B Kovari, I Lepran, P Ferdinandy, Z Kahan
Journal: Cardiovascular Research (2014)

Purpose: Due to the clinical significance of radiogenic heart damage, it is important to investigate it with clinical and biological methods. Our aim was to develop reliable in vitro and in vivo animal models which help to study the effect of radiation to the heart and to test potential radiation protective materials. Materials and Methods: In in vitro experiments, cardiomyocyte cells were isolated and cultured from newborn rats. The 24-hour cultures were exposed to different doses (5, 10, 15, 20 Gy) of radiation, and the effect was examined at different latency times (0, 24, 48, 72, 96, 120 h). The cells were cultured in a medium containing 1% fetal bovine serum (FBS), but the experiments were completed in serum-free conditions, too. The toxicity of the radiation was evaluated with calcein fluorescence viability assay. In our in vivo model using adult male rats, we developed a selective heart irradiation technique and determined the dose effect curve. The irradiation was carried out with 20, 25, 30 and 40 Gy. After the irradiation animals were continuously monitored for 3 months (weight, heart ultrasound, measurement of circulating cytokines), and at the end of the third month, they were sacrificed and pathological analyses was performed on the cardiac and lung tissues. Results: In the in vitro experiments, 48 hours after 10 Gy irradiation, 50% of the myocytes, grown in medium containing serum were lost, thus, these circumstances seemed to be the most suitable for the study of the radiation damage. We examined the survival of the cardiac muscle cells in serum-free environment, in this case the radiation dose and latency required for a 50%-cell loss were 10 Gy and 24 hours, respectively. In the in vivo model, echocardiography indicated septal wall motion disturbance in animals receiving a radiation dose of 40 Gy 3 months after the treatment. With histological examination, significant myocardial or vascular damage was not found, however, in animals irradiated with the highest dose (40 Gy) mild radiation pericarditis, fibrotic heart lesions and lung damage were observed by hematoxylin-eosin and Crossman's trichrome staining. Conclusions: In accordance with our initial results, the described in vitro and in vivo models appear to be suitable for studying the mechanisms of radiogenic heart damage. In the following experiments, we intend to raise the radiation dose and to extend the time of observation in order to better evaluate the mechanisms of action of radiation and the effects of radiation protective materials.
Standard Medical School Hardest Hit by Indirect Cost Cuts
Authors: C. Vanchieri
Journal: JNCI Journal of the National Cancer Institute (1991)

Standard Medical School Hardest Hit by Indirect Cost Cuts Cori Vanchieri Cori Vanchieri Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 83, Issue 12, 19 June 1991, Page 823, https://doi.org/10.1093/jnci/83.12.823 Published: 19 June 1991
Abstract P3-10-01: Epidemiological risk factors and normal breast tissue markers in inflammatory breast cancer
Authors: RL Atkinson, KR Sexton, NT Ueno, R El-Zein, AM Brewster, SA Krishnamurthy, WA Woodward
Journal: Cancer Research (2012)

Abstract Background: Inflammatory breast cancer (IBC) is a rare form of aggressive breast cancer with no existing identifiers for screening or prevention strategies. Women with triple-negative (TNBC, ER–, PR–, Her2–) non-inflammatory breast cancer are less likely to breastfeed, and we have shown in adjacent normal breast tissue that this tissue has more foci of stem cells compared to non-TNBC cancers. A disproportionately higher percentage of women with IBC have TNBC relative to women with non-IBC. We hypothesized that adjacent normal tissue in TNBC IBC vs. TN non-IBC may also display unique biological features based on epidemiologic characteristics. Methods: We examined epidemiologic factors by breast cancer receptor subtype in 144 patients diagnosed with IBC in 1991–2011 at MD Anderson Cancer Center. Breast cancer risk factors including parity and breastfeeding were compared between patients with TN and non-TN IBC with chi-square or Wilcoxon rank sum tests. Normal adjacent tissues were stained for stem cell markers CD44+CD49f+CD133/2+ and macrophage marker CD68. Results: The mean age at diagnosis was 52.3 years (range = 23–80) and 83% of patients were non-Hispanic white, 80% were overweight or obese (BMI &amp;gt;25), and 36% were TN IBC. Patients with TN IBC had significantly lower frequency of breastfeeding compared with non-TN IBC, 28% vs. 55%, (p = 0.01). No differences were found in the frequency of other breast cancer risk factors. All 8 IBC adjacent tissue samples showed a distinct spatial distribution of stem cell staining, not limited to the triple negative patients. Compared with 0/60 non-IBC cases, 0/8 triple negative non-IBC, (p = 0.001 Pearson chi-square). Given the high BMI among IBC patients, we further examined normal tissues for the presence of CD68+ cells distributed individually or as clusters exhibiting a “crown-like” pattern (multiple CD 68+ macrophages found around dead adipocytes), and found that 7 of the 8 IBC adjacent tissues were CD68+. Benign biopsies collected from 2 patients at 10 years before diagnosis displayed similar staining, including both stem cell and CD68 staining. Compared with 12/60 non-IBC adjacent tissues were positive for CD68, with 1/8 TN non-IBC, (p = 0.001 Pearson chi-square). Conclusion: We describe for the first time a stem-cell staining pattern unique to IBC present in all IBC tissues examined, including pre-cancer biopsies. Tissue samples from additional patients will be examined to further explore the relationship between stem cells and CD68 positivity with IBC subtypes. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P3-10-01.
Psoriasis with Acro-Osteolysis
Authors: William R. Buckley, Robert L. Raleigh
Journal: New England Journal of Medicine (1959)

ACRO-osteolysis, without associated articular inflammation, has been observed in a patient presenting cutaneous lesions of psoriasis. Lysis of bone followed trauma to the distal aspect of the involved phalanges. This finding adds to the evidence already available that psoriasis is a systemic disease.1 Its peculiar localization to various tissues is "triggered" by trauma whether chemical or physical. Certainly, its localization to the cutaneous system after physical stimuli was noted over a hundred years ago by many observing physicians, and particularly, by Kobner,2 , 3 in 1872. Localization in articular tissue, as in psoriatic arthritis, has been of great interest to the rheumatologists, . . .
P1207 TRIPLE THERAPY FOR HCV CIRRHOSIS IN LIVER TRANSPLANT CANDIDATES. HIGH RATE OF PREVENTION OF HCV RECURRENCE AFTER LIVER TRANSPLANTATION (LT) IN WELL SELECTED PATIENTS
Authors: A. Braithwaite, V. Aguilera, C. Vinaixa, M. Garcia, S. Benlloch, A. Rubin, M. Prieto, M. Berenguer
Journal: Journal of Hepatology (2014)
THU0443 Differential diagnosis between rheumatoid arthritis and peripheral spondylarthropathies: Usefulness of shoulders ultrasonography
Authors: S. Ottaviani, K. Dawidowicz, E. Palazzo, G. Gill, G. Hayem, O. Meyer, P. Dieudé
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The differential diagnosis between rheumatoid arthritis (RA) and peripheral spondylarthropathies (pSpA) could be difficult. A recent ultrasound study showed that ultrasonography (US) of hands might help the physician to differentiate psoriatic arthritis to RA (1). <h3>Objectives</h3> To investigate the usefulness of shoulders US to differentiate RA and pSpA, in individuals having painful shoulders. <h3>Methods</h3> 36 RA (EULAR/ACR 2010 criteria) patients and 32 SpA (ESSG criteria) patients with clinical involvement of shoulder joints were consecutively included in the study. All US examinations were performed by one trained rheumatologist. The following items were systematically assessed: gleno-humeral effusion, long head biceps tendon tenosynovitis, sub-acromial and sub-deltoid bursitis, acromio-clavicular synovitis and bone erosion. <h3>Results</h3> A total of 36 RA patients (median age: 56.0 [44.5-62.4], 72% of female) and 32 SpA patients (median age: 45.5 [37.8-58.3], 47% of female) were assessed. Rheumatoid Factor and anti-CCP antibodies were found in 86% and 91% in RA patients, respectively and none in SpA patients. A higher frequency of acromio-clavicular synovitis was observed in pSpA (72% vs 6%, OR=43.4 [7.6-329.4], P&lt;0.0001). RA patients had more frequently sub-acromial and sub-deltoid bursitis (67% vs 31%, OR=4.4 [1.4-14.0], P=0.008), long head biceps tendon tenosynovitis (47% vs 13%, OR=6.2 [1.6-26.3], P=0.005), gleno-humeral effusion (28% vs 3%, OR =11.9 [1.4-265.6], P=0.015) and bone erosion (53% vs 3%, OR=35.6 [4.1-755.9], P&lt;0.0001). <h3>Conclusions</h3> Our results suggest that painful shoulder is highly associated with the acromio-clavicular synovitis detected by US in pSpA. Thus, US might help the physician i) to differentiate RA and pSpA in patients with painful shoulders ii) to better define the structure requiring local corticosteroid injection. <h3>References</h3> Gutierrez M, Filippucci E, Salaffi F, Di Geso L, Grassi W.Differential diagnosis between rheumatoid arthritis and psoriatic arthritis: the value of ultrasound findings at metacarpophalangeal joints level. Ann Rheum Dis. 2011 Jun;70(6):1111-4. Epub 2011 Mar 15. <h3>Disclosure of Interest</h3> None Declared
Reply to Dr. Sahai
Authors: Shinpei Doi, Ichiro Yasuda
Journal: Endoscopy (2013)

We appreciate the comments made by Dr. Sahai regarding our study in which we concluded that endoscopic ultrasound (EUS)-guided celiac ganglia neurolysis (EUS – CGN) is more effective than the “central” technique of EUS-guided celiac plexus neurolysis (EUS – CPN) [1]. Since EUS – CPN (the central technique) was first introduced by Wiersema et al. [2], some modified techniques have been developed, which have aimed to increase the spread of the drug to a wider area than that achieved by the original central technique; moreover, the outcomes of the new techniques have been shown to be superior to those of the central technique [3] [4].
Basic mechanisms of ovarian function: germ cells
Authors: Stephen E. Harris
Journal: Environmental Health Perspectives (1978)

Recent work is reviewed on several molecular aspects of gene expression during oocyte maturation and early development. Several analyses have been carried out on protein synthesis patterns using the high resolution two-dimensional polyacrylamide gel electrophoresis technique. The protein pattern of some 400–500 spots in sea urchin oocytes before and just after fertilization are quite similar. By gastrula, major stage specific protein changes have been noted. In a similar study major protein changes are noted during the meiotic maturation process in mammals. With the use of the single-copy DNA hybridization technique, the quantitative levels of rare mRNA sequence expression have been determined during oogenesis and early development in the sea urchin model. The mature oocyte sequence set of some 37 × 106 nucleotides of information (or potentially 18,500 different proteins) is synthesized during oogenesis and slowly utilized during development. The sea urchin gastrula mRNA is predominantly synthesized by the embryo genome; however, essentially all those gastrula mRNA sequences can also be found in the mature oocyte (maternal) set. It is proposed by Hough-Evans et al. that this maternal sequence set, which is made during oogenesis and both utilized (translated in 10,000–20,000 proteins), and in part, continually synthesized by all stages of the embryo, plays a critical role in the morphogenic formation of a sea urchin embryo.
Abstract 5340: MicroRNA-720 promotes cell proliferation and invasion in prostate cancer cells.
Authors: Soichiro Yamamura, Takeshi Chiyomaru, Shinichiro Fukuhara, Sharanjot Saini, Shahana Majid, Guoren Deng, Vary Shahryary, Sumit Arora, Inik Chang, Yuichiro Tanaka, Peter Carroll, Rajvir Dahiya
Journal: Cancer Research (2013)

Abstract MicroRNAs (miRNAs) are highly conserved, single stranded, non-coding RNAs of approximately 21 - 24 nucleotides that regulate gene expression by posttranscriptional silencing of specific target RNAs, by repressing translation or cleaving RNA transcripts by binding to the 3’ untranslated region (3’UTR) of the target messenger RNAs. miRNAs regulate diverse cellular processes such as cell-cycle progression, proliferation, apoptosis and development. miRNAs also have been shown to function as oncogenes or tumor suppressor genes. MicroRNA profiling has shown that miR-720 was up-regulated in cancer tissues, however the function of miR-720 in cancer has not been studied. Here we studied the functional effects of miR-720 on prostate cancer cells. We found that miR-720 was up-regulated in human prostate cancer tissues compared to adjacent normal tissues. We also found that miR-720 was up-regulated in prostate PC-3 cancer cell line compared with normal prostate cell line, RWPE1 cells. Transfection of miR-720 into prostate cancer cells, PC-3 and DU145 cells, decreased apoptosis, promoted cell proliferation and cell invasion. Transfection of anti-miR-720 into PC-3 cells increased apoptosis, inhibited cell proliferation and cell invasion. These results indicated that miR-720 has oncogenic functions in prostate cancer cells. In-silico analysis suggests that miR-720 targets genes of DR1, a down-regulator of global transdcription, and SWI5, a component of a complex required for double-strand break repair, which have tumor suppressive functions. Luciferase assays indicated that miR-720 binds to the 3’ UTR of these genes in prostate cancer cells, suggesting that these genes are targets of miR-720. Our results suggest that miR-720 promotes malignancy by targeting DR1 and SWI5 in prostate cancer cells. Funding: National Institutes of Health through Grant Number RO1CA138642, RO1CA130860 and RO1CA160079 and VA Merit Review and VA Program Project. Citation Format: Soichiro Yamamura, Takeshi Chiyomaru, Shinichiro Fukuhara, Sharanjot Saini, Shahana Majid, Guoren Deng, Vary Shahryary, Sumit Arora, Inik Chang, Yuichiro Tanaka, Peter Carroll, Rajvir Dahiya. MicroRNA-720 promotes cell proliferation and invasion in prostate cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5340. doi:10.1158/1538-7445.AM2013-5340
Prostate Cancer Rates Climbed Sharply in 1990
Authors: T. Reynolds
Journal: JNCI Journal of the National Cancer Institute (1993)

Journal Article Prostate Cancer Rates Climbed Sharply in 1990 Get access Tom Reynolds Tom Reynolds Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 85, Issue 12, 16 June 1993, Pages 947–948, https://doi.org/10.1093/jnci/85.12.947 Published: 16 June 1993