tbuckley/cabot-search huggingface dataset:
Cytotoxic T Cells in Cytomegalovirus Infection
Authors: Gerald V. Quinnan, Nigar Kirmani, Alain H. Rook, Jody F. Manischewitz, Lozannie Jackson, Gail Moreschi, George W. Santos, Rein Saral, William H. Burns
Journal: New England Journal of Medicine (1982)

We studied 58 recipients of bone-marrow transplants to evaluate immune responses to cytomegalovirus infection. Such infection developed in 43 patients; it was fatal in 12, nonfatal in 23, and present at death from other causes in eight. All patients had low or absent cytomegalovirus-specific cytotoxic lymphocyte activity before the onset of infection. Cytomegalovirus-specific cytotoxic responses developed in all survivors, whereas only two patients with fatal infection had even low-level cytomegalovirus-specific cytotoxic responses. Natural and antibody-dependent killer-cell activities were depressed both before and during infection in patients with fatal infections, but not in those who survived. The outcome of the infection did not correlate with the nature of the underlying disease, the type of transplant received, the pretransplantation cytomegalovirus-antibody status, or lymphocyte-proliferation responses to cytomegalovirus antigens or concanavalin A. The correlation between effective virus-specific cytotoxic response and recovery from infection indicates that these effector cells probably mediate recovery from cytomegalovirus infection. (N Engl J Med. 1982; 307:6–13.)
Abstract 5286: Evaluation of 64Cu-labeled peptide for in vivo imaging of Thomsen-Friedenreich antigen expressing breast carcinomas
Authors: Senthil R. Kumar, Thomas Quinn, Susan Deutscher
Journal: Cancer Research (2011)

Abstract Introduction: Thomsen-Friedenreich carbohydrate antigen (TF; Galβ1-3GalNAc) is displayed on cell surface proteins and lipids in ∼90% of adenocarcinomas including those of the breast. We investigated the ability of a peptide, DRD-IVWHRWYAWSPASRI-DRD-NH2 (TF-pep), selected through bacteriophage display to bind breast and prostate carcinoma cells expressing TF. It is hypothesized that TF-pep once radiolabeled with 64Cu, a positron radiation emitter, has the potential to be used as a positron emission tomography (PET) imaging agent for TF expressing breast tumors for diagnostic purposes. Experimental Procedure: A bifunctional-chelator (1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA)) was attached to the TF-pep with Gly-Ser-Gly (GSG) spacer. The peptide was radiolabeled with 64Cu in ammonium acetate buffer pH 6.8 at 80oC for 60 minutes. In vitro cell binding studies with both human breast (MDA-MB-435) carcinoma cells, as well as normal breast epithelial (184A1) cells were performed with the radiolabeled peptide. In vivo biodistribution and PET/CT imaging of the tumor with radiolabeled peptide was analyzed in SCID mice bearing MDA-MB-435 tumor xenografts. Results: 64Cu-NOTA-Gly-Ser-Gly-TF-pep peptide bound to human MDA-MB-435 carcinoma cells in a time dependant manner while insignificant binding was observed with normal breast 184A1 cells. In vivo biodistribution studies demonstrated radiolabeled peptide accumulation in MDA-MB-435 tumor-bearing SCID mice (∼1.09 ± 0.34 %, and 0.85 ± 0.06% ID/g in tumor) at 0.5 h and 2 h, respectively. Little accumulation occurred in other organs with the exception of liver (1.12 ± 0.12% ID/g, and kidneys (15.4 ± 1.2% ID/g) at 1 h. Live imaging studies with 64Cu-NOTA-Gly-Ser-Gly-TF-pep (300 μιχρoCi) demonstrated good tumor uptake after 1 h post-injection. Conclusions: The TF-specific peptide once radiolabeled with 64Cu, can function as a non-invasive in vivo tumor imaging agent of human breast and other carcinomas expressing this antigen. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5286. doi:10.1158/1538-7445.AM2011-5286
Anti-Donor Immune Responses in Prediction of Transplant Rejection
Authors: Calvin R. Stiller, Nicholas R. StC. Sinclair, Sydney Abrahams, Dolores McGirr, Harinderjit Singh, William T. Howson, Raymond A. Ulan
Journal: New England Journal of Medicine (1976)

We assessed various immune responses against donor tissue to determine their value in the diagnosis and prediction of clinical rejection episodes. Twenty-six consecutive clinical renal-transplant recipients were examined. Cell-mediated lymphocytotoxicity preceded and accompanied 41 of 45 rejection episodes (P less than 0.001). Complement-dependent antibody was present in 12 of 15 rejections (P less than 0.002)--four not accompanied by, and eight in association with, cell-mediated lymphocytotoxicity. Mixed lymphocyte reactivity or nonreactivity and inhibition by autologous serum occurred equally often in rejection and quiescence. Lymphocyte-dependent antibody occurred during both rejection episodes and quiescent phases, with a greater frequency during quiescence (P = 0.05). Cell-mediated lymphocytotoxicity was the best predictor of rejection (P less than 0.05). Cell-mediated lymphocytotoxicity was the best predictor of rejection (P less than 0.001), and was more easily suppressed by standard immunosuppressive therapy, than complement-dependent antibody. If specific cell-mediated lymphocytotoxicity, with or without antibody, recurred after rejection therapy, the graft underwent further rejection.
Two cases of acute severe flaccid myelitis associated with enterovirus D68 infection in children, Norway, autumn 2014
Authors: H C Pfeiffer, K Bragstad, M K Skram, H Dahl, P K Knudsen, M S Chawla, M Holberg-Petersen, K Vainio, S G Dudman, A M Kran, A E Rojahn
Journal: Eurosurveillance (2015)

Enterovirus D68 (EV-D68), phylogenetic clade B was identified in nasopharyngeal specimens of two cases of severe acute flaccid myelitis. The cases were six and five years-old and occurred in September and November 2014. EV-D68 is increasingly associated with acute flaccid myelitis in children, most cases being reported in the United States. Awareness of this possible neurological complication of enterovirus D68 infection is needed.
S88 Day case cisplatin delivery for advanced NSCLC patients: faster, cheaper, more desirable
Authors: H. Jarrett, M. Hill, L. J. Billingham, K. J. O'Byrne, D. Ferry
Journal: Thorax (2011)

<h3>Background</h3> The BTOG2 trial was a phase III randomised clinical trial in the treatment of advanced NSCLC. It investigated the optimal dose of cisplatin (50 vs 80 mg/m<sup>2</sup> 3-weekly), in combination with gemcitabine, and whether carboplatin (AUC6-Wright) could safely and effectively be substituted for the optimal cisplatin dose. The protocol recommended cisplatin given as an out-patient regimen designed to ensure diuresis while maintaining electrolytic balance. A previously reported audit by these authors, 48% of hospitals surveyed were admitting NSCLC patients for cisplatin /gemcitabine chemotherapy. <h3>Methods</h3> Between April 2005 and November 2009, 909 patients were randomised to receive cisplatin, in the UK and Ireland, as part of BTOG2. The trial mandated submission of proposed chemotherapy delivery schedules to ensure standard parameters in terms of: total duration of delivery, mandatory use of mannitol, short 1-h delivery of cisplatin and total fluid volume &lt;4 l. Data mining was used to investigate AEs relating to renal function, electrolyte imbalance and ototoxicity. AEs that could feasibly be related to the manner in which cisplatin was administered. <h3>Results</h3> 2853 treatment cycles were available for analysis. Average treatment duration decreased from nearly 9 to 6 h and total fluid volume from as much as 7 to &lt;4 l. As a result of participating in BTOG2, 97% of surveyed hospitals were able to deliver cisplatin in a day case setting. Toxicities feasibly related to the manner in which cisplatin was administered were comparable to the current available literature with &lt;1% experiencing grade &gt;2. <h3>Conclusion</h3> Current NHS Tariffs in the UK quote a 60% higher price for patients being inpatient cisplatin treatment as opposed to outpatient. With the prima facie case that patients prefer outpatient treatment, it is important to achieve the maximum benefit from the existing drugs in a clinically deliverable way. The results indicate that administering cisplatin via a short hydration schedule of &lt;6 h, even at 80 mg/m<sup>2</sup>, is safe. It is unlikely that the many hospitals who changed their practice would have done so without the support of a running randomised controlled trial.
THE PREDICTIVE VALUE OF 201TL-123I-BMIPP SCINTIGRAPHY IN THE RECOVERY OF LEFT VENTRICULAR SYSTOLIC DYSFUNCTION IN TAKOTSUBO SYNDROME
Authors: Takayuki Warisawa, Nobuo Tomizawa, Toru Naganuma, Yusuke Fujino, Hisaaki Ishiguro, Satoko Tahara, Naoyuki Kurita, Nakamura Shotaro, Takeshi Nojo, Sunao Nakamura
Journal: Journal of the American College of Cardiology (2016)
SAT0410 Determinants of Hypertension in Patients with Psoriatic Arthritis
Authors: R. Queiro, B. Acasuso, S. Rodríguez, I. Cabezas, I. Morante, P. Tejόn, L. Arboleya, J.L. Riestra
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Several studies have found a higher rate of hypertension among patients with psoriasis and psoriatic arthritis (PsA) versus the general population. This frequency may be even higher in patients with PsA compared to those with psoriasis alone<sup>1</sup>. Risk factors linked to the development of hypertension in PsA are unknown. <h3>Objectives</h3> To analyze the clinical profile and risk factors linked to hypertension in subjects with PsA. <h3>Methods</h3> Retrospective cohort study including 205 patients with PsA (CASPAR criteria) followed in a single university center. Cohort comprised by 112 men and 93 women, mean age 53±12.9 years. Average duration of psoriasis of 22±10.2 years and 14±8.9 years for arthritis. The following variables were included in all patients: age of onset of psoriasis and PsA, educational level, type and location of psoriasis, form of onset of PsA, evolutionary pattern, enthesitis, nail disease, DIP involvement, dactylitis, erosions, HLA-B27 and C*06, BMI, NSAID/GC and systemic therapy. Univariate and multivariate analyses were performed. <h3>Results</h3> Sixty three of the 205 subjects had hypertension (30.7%). There was no difference in the frequency of hypertension between men and women. In univariate analysis, factors related to hypertension were low educational level (p&lt;0.0001), pustular psoriasis (p=0.040), polyarticular onset (p=0.012), polyarticular evolution (p=0.040), onset of psoriasis ≥40 years (p&lt;0.001), onset of arthritis ≥40 years (p=0.004) and obesity (p=0.001). However, in the multivariate analysis the only factor linked to hypertension risk was age (OR 1.12, 95% CI: 1.07-1.18). <h3>Conclusions</h3> Hypertension is common among patients with PsA. Some clinical features of the disease linked to its inflammatory burden (polyarticular forms), social status (low educational level) or other comorbidities (obesity), seem to increase this risk. In any case it seems that the main determinant of this comorbidity is the age of patients (risk increase with age). <h3>References</h3> Miller IM, Ellervik C, Yazdanyar S, Jemec GB. Meta-analysis of psoriasis, cardiovascular disease, and associated risk factors. J Am Acad Dermatol. 2013 Dec;69(6):1014-24. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2285
Abstract A47: Possible effect of repetitive magnetic stimulation of the spinal cord on the limb angiogenesis in healthy rats and its clinical implication for the treatment of lymphedema
Authors: Yusun Min, Jaewon Beom, Byung-Mo Oh, Kwan-Sik Suh
Journal: Clinical Cancer Research (2010)

Abstract Objective: To investigate the effect of repetitive magnetic stimulation of the spinal cord on limb angiogenesis in healthy rats and explore its implication for the treatment of lymphedema. Methods: Twelve adult male Sprague-Dawley rats were divided into four groups as follows: sham repetitive magnetic stimulation (rMS) followed by tissue harvest 5 minutes later (group 1, n=2), 1 Hz rMS and tissue harvest 5 minutes later (group 2, n=3), 20Hz rMS and tissue harvest 5 minutes later (group 3, n=3), 20Hz rMS and tissue harvest 30 minutes later (group 4, n=4). Animals were treated with 20-minute rMS with 120% of the motor threshold on their left side of upper lumbar spinal cord. Expression of angiogenic factors, that is, Akt, phospho-Akt, endothelial nitric oxide synthase (eNOS), phospho-eNOS were measured by Western blot. Bilateral hindlimb muscles (quadriceps and gastrocnemius) were harvested. Results: Expression of eNOS in quadriceps muscle was markedly increased in group 3 and 4 (6.4 and 7.7-fold each compared with group 1, p=0.04). Also, expression of eNOS in gastrocnemius muscle was increased in group 2 and 3 than in group 1 (p=0.02). Expression of Akt in gastrocnemius muscle tends to increase in rMS- treated groups than in sham rMS group (p&amp;gt;0.05). Expression of phospho-Akt and phospho-eNOS were not comparable between four groups (p&amp;gt;0.05). Conclusion: Repetitive magnetic stimulation of the spinal cord may exert an angiogenic effect closely linked to lymphangiogenesis. It has clinical implication for the possible therapy of lymphedema caused by breast, cervical or endometrial cancer operation. Future studies with the specific lymphatic endothelial cell markers are required to confirm the effect of repetitive magnetic stimulation on lymphangiogenesis. Citation Information: Clin Cancer Res 2010;16(14 Suppl):A47.
Segmental Hepatic Concentration of 18F-FDG-Labeled Autologous Leukocytes Causing a “Pseudo-Lesion” on PET/CT in a Patient With Transient Hepatic Attenuation Difference
Authors: Anish Bhattacharya, Raghava Kashyap, Rakesh Kochhar, Sarika Sharma, Naveen Kalra, Bhagwant Rai Mittal
Journal: Clinical Nuclear Medicine (2013)

Labeled leukocyte scintigraphy has been described in the detection of infection in peripancreatic fluid collections in patients with acute pancreatitis. The authors describe a sectorial hepatic concentration of 18F-FDG-labeled autologous leukocytes forming a “pseudo-lesion” on PET/CT, matching the characteristic finding of transient hepatic attenuation difference on venous phase contrast-enhanced computerized tomography.
Gastroscopic snare polypectomy for cystic duct adenoma: a rare occurrence
Authors: Zhong-hong Liu, Cheng-qian Lv, Guang-xing Cui, Xu Zhang, Kavanjit Kaur, Mei-ling Han, Zhi-wu Lv
Journal: Endoscopy (2014)

Cystic duct adenoma is an uncommon disease. Appropriate therapeutic strategies have not been clearly defined. Surgical resection is often recommended [1] [2] [3] [4]. Recently, endoscopic procedures for biliary papillomatosis were reported, such as endoscopic papillary balloon dilation, argon plasma coagulation, photodynamic therapy, endoscopic retrograde biliary drainage, and intraluminal brachytherapy with iridium-192 [5] [6] [7] [8] [9]. However, it is difficult to achieve complete resection and the recurrence rate is high. We report on a patient diagnosed with cystic duct adenoma who presented with recurrent cholangitis and was treated with a snare polypectomy using a gastroscope.
A Different Perspective on Thrombosis and the HeartMate II
Authors: John M Stulak, Simon Maltais
Journal: New England Journal of Medicine (2014)

This study confirms previous observations of an increased risk of pump thrombosis with the HeartMate II LVAD beginning in 2011. However, this study shows that after a peak in 2012, the risk of pump thrombosis returned to baseline by 2014.
AB0070 The Importance of Gliostatin as an Indicator of Disease Activity in Patients with Rheumatoid Arthritis
Authors: K. Ikuta, Y. Waguri-Nagaya, N. Tatematsu, Y. Kawaguchi, M. Kobayashi, M. Aoyama, K. Asai, T. Otsuka
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Gliostatin (GLS) plays an important role in joint destruction in rheumatoid arthritis (RA). GLS not only has an enzymatic activity as a thymidine phosphorylase but also has angiogenic and arthritogenic activities. We previously reported that GLS levels in serum and synovial fluid are increased in RA patients compared with healthy controls and in osteoarthritis patients<sup>1</sup>. In addition, the production of GLS by cultured RA fibroblast-like synoviocytes (FLSs) has been shown to be enhanced by IL-1β, TNF-α and GLS itself<sup>2</sup>. GLS levels in TNF-α-stimulated FLSs were reduced by FK506 treatment<sup>3</sup>. <h3>Objectives</h3> The aim of this study was to investigate whether GLS serum levels were correlated with disease activity in RA patients. In addition we evaluated the effects of TNF inhibitors and the immunosuppressant FK506 (tacrolimus) on serum GLS levels. <h3>Methods</h3> Serum GLS levels were measured using an enzyme immunoassay system in 146 samples from 21 Japanese RA patients. Correlations were analysed between serum GLS levels and clinical features, laboratory results and disease activity scores (DAS) as defined by EULAR. <h3>Results</h3> We identified positive associations between serum GLS levels in RA patients and C-reactive protein (CRP), matrix metalloproteinase-3 (MMP-3), and DAS 28(ESR). After TNF inhibitor treatment for 12 months, 9 of 10 patients showed a good or moderate clinical response and also showed significant reductions in serum GLS levels and other laboratory parameters. After FK506 treatment for 12 weeks, 6 of 11 patients showed good or moderate responses, according to DAS 28(ESR), while 5 patients showed no response. Serum GLS levels and other laboratory parameters were only reduced significantly in the 6 responders. <h3>Conclusions</h3> Circulating GLS levels were significantly associated with CRP, MMP-3, and DAS28(ESR). TNF inhibitors and FK506 may suppress serum levels of GLS and thus its angiogenic and arthritogenic activity. Our results suggested a possible role for GLS in RA pathogenesis. <h3>References</h3> Takeuchi, et al.: Aberrant production of gliostatin/platelet-derived endothelial cell growth factor in rheumatoid arthritis. Arthritis Rheum 1994; 37: 662–72. Muro H, et al. Autocrine induction of gliostatin/platelet-derived endothelial cell growth factor (GLS/PD-ECGF) and GLS-induced expression of matrix metalloproteinases in rheumatoid arthritis synoviocytes. Rheumatol 1999; 38: 1195 -1202. Yamagami T, et al. FK506 inhibition of gliostatin/thymidine phosphorylase production induced by tumor necrosis factor-α in rheumatoid fibroblast-like synoviocytes. Rheumatol Int 2011; 37:903-909. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2315
Psychiatric Consultation in Somatization Disorder
Authors: G. Richard Smith, Roberta A. Monson, Debby C. Ray
Journal: New England Journal of Medicine (1986)

The per capita expenditure for health care of patients with multiple physical symptoms but no apparent physical disease (somatization disorder) is up to nine times the average per capita amount. We conducted a randomized controlled trial to determine whether psychiatric consultation would reduce the medical costs of these patients, without effecting a substantial change in patient outcome. Thirty-eight patients were randomly assigned to treatment or control groups and studied prospectively for 18 months. Treatment consisted of a psychiatric consultation and suggestions on management given to primary physicians. After nine months, the control group was crossed over to receive treatment with the same intervention. After the psychiatric consultation, the quarterly health care charges in the treatment group declined by 53 percent (P<0.05). In contrast, the charges in the control group showed wide variations but no overall change. The quarterly charges in the control group were significantly higher than those in the treatment group (P<0.05). After the control group was crossed over to receive treatment, their quarterly charges declined by 49 percent (P<0.05). The reductions in expenditures in both groups were due largely to decreases in hospitalization. We conclude that psychiatric consultation in the care of patients with somatization disorder reduced subsequent health care expenditures without inducing changes in health status or patients' satisfaction with their health care. (N Engl J Med 1986; 314:1407–13.)
Abstract 165: MIRA (methylated CpG island recovery assay) assay identifies frequently methylated genes in breast cancer
Authors: Victoria Hill, Luke Hesson, Temuujin Dansranjavin, Ashraf Dallol, Ivan Bieche, Stella Tommasi, Timothy Dobbins, Dean Gentle, David Euhus, Reinhard Dammann, Robyn L. Ward, John Minna, Eammon R. Maher, Gerd Pfeifer, Farida Latif
Journal: Cancer Research (2010)

Abstract There are several high throughput approaches to identify methylated genes in cancer. We utilized one such recently developed approach, MIRA (methylated-CpG island recovery assay) combined with CpG island arrays to identify novel genes that are epigenetically inactivated in breast cancer. Using this approach we identified numerous CpG islands that demonstrated aberrant DNA methylation in breast cancer cell lines. Using a combination of combined bisulphite restriction analysis (CoBRA) and sequencing of bisulfite modified DNA, we confirmed 5 novel genes frequently methylated in breast tumors; EMILIN2, SALL1, DBC1, FBLN2 and CIDE-A. Methylation frequencies ranged from between 25% and 63% in primary breast tumors, whilst matched normal breast tissue DNA was either unmethylated or demonstrated a much lower frequency of methylation compared to malignant breast tissue DNA. Furthermore expression of the above 5 genes was shown to be restored following treatment with a demethylating agent in methylated breast cancer cell lines. We have expanded this analysis across three other common epithelial cancers (lung, colorectal and prostate). We demonstrate that the above genes show varying levels of methylation in these cancers. Lastly and most importantly, methylation of EMILIN2 was associated with poorer clinical outcome in breast cancer and was strongly associated with estrogen receptor as well as progesterone receptor positive breast cancers. The combination of the MIRA assay with CpG island arrays is a very useful technique for identifying epigenetically inactivated genes in cancer genomes and can provide molecular markers for early cancer diagnosis, prognosis and epigenetic therapy. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 165.
Abstract IA17: Microfluidic measurements of single cell mass reveal how growth and division are coordinated
Authors: Scott Manalis
Journal: Cancer Research (2011)

Abstract Cell growth during the cell cycle must be coordinated with genome replication and segregation, but the mechanisms underlying this coordination are poorly understood, particularly in mammalian cells. My lab is developing suspended microchannel resonators that can precisely measure the size of single cells – mass, volume and density – over time. Using a microfluidic system for culturing single cells over multiple generations while cell mass and cell cycle progression were continuously measured, we monitored over 1,000 hours of single cell growth from mouse lymphoblasts (L1210). I will present recent data from this system and discuss preliminary experiments where the precision cell sizing capabilities of the resonator are used for biomarker analysis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the Second AACR International Conference on Frontiers in Basic Cancer Research; 2011 Sep 14-18; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2011;71(18 Suppl):Abstract nr IA17.