tbuckley/cabot-search huggingface dataset:
PTU-288 Taking the ibd standards forward in scotland: a national patient survey
Authors: AJM Watson
Journal: Gut (2015)

<h3>Introduction</h3> Crohns and Colitis UK, working with Scottish Government, have commited to a project to raise the standards of care for patients with Inflammatory Bowel Disease (IBD) in Scotland. The programme’s objective is to develop a national strategy for IBD service improvement, led by a national steering group supported by the findings from pilots in two diverse Health Boards. As part of the process, a large national survey was commissioned. The aim of the study, therefore, was to present the data generated from 777 partcipants who responded to the survey. <h3>Method</h3> An on line questionnaire was developed and completed by people living in Scotland who have IBD. The survey was a snap shot of their views and experiences of their NHS health care provision, with a particular focus on their most recent flare up. The survey contained a combination of quantitive and qualitative (reported seperately) questions. The survey was designed to be completed on-line (Survey Monkey) and was distributed through the Crohns and Colitis UK website and membership database. Social digital networks were also used to promote the survey. The survey was conducted between March and May 2013. <h3>Results</h3> 777 particpants responded to the survey (68% female). The majority of respondents were aged 16–65 (88%). 64% said that they had an IBD nurse. 79% of patients had an annual review. This review was principally by a gastroenterologist (78%). 50% of patients had suffered a disease flare in the previous 6 months. Principal symptoms of the last flare will be presented. 19% of patients self-treated during their last flare, 41% contacted their GP and 33% contacted their IBD nurse. Patients would like to have had a referral to an Occupational therapist, careers adviser and social worker. over 328 patients took time off during their last flare, with 40% taking over a week off. <h3>Conclusion</h3> This survey provides confirmation that there are many people with IBD living in Scotland whose NHS service provision falls below that outlined in the IBD Standards. Prompt diagnosis, readily available information, quick access to advise and support are issues that are frequently raised. The results of the survey have informed the pilot Health Boards, who have developed workstreams to address areas of deficiencies, <h3>Disclosure of interest</h3> None Declared.
The fate of patients referred to a specialistvascular unit with large infra-renal abdominalaortic aneurysms over a two-year period
Authors: Nida Pasha, Sharanya Kumar, Alan Karthikesalingam, Zaki Akhtar, T. Nicoli, Peter Holt, Robert Hinchliffe, Ian Loftus, Matt Thompson
Journal: International Journal of Surgery (2012)
Arterial Foci of F-18 Fluorodeoxyglucose Are Associated With an Enhanced Risk of Subsequent Ischemic Stroke in Cancer Patients
Authors: Solène Grandpierre, Emmanuel Desandes, Benoit Meneroux, Wassila Djaballah, Damien Mandry, Fanny Netter, Denis Wahl, Renaud Fay, Gilles Karcher, Pierre-Yves Marie
Journal: Clinical Nuclear Medicine (2011)

Imaging of F-18 fluorodeoxyglucose (FDG) by positron emission tomography/computed tomography (PET/CT) hybrid systems allows detecting arterial FDG foci, which relate to inflammatory and presumably unstable atherosclerosis, and is increasingly used in cancer patients among whom many are at risk for ischemic events. However, the link between an enhanced arterial FDG uptake and subsequent ischemic events remains to be clearly established. This pilot study aimed at determining whether the prior arterial FDG uptake in cancer patients presenting a subsequent stroke was higher than that of stroke-free controls.Patients referred to FDG PET/CT for conventional oncologic indications were retrospectively included and compared between: (i) 7 case-patients with subsequent hospitalizations for documented ischemic stroke and (ii) 16 event-free controls, matched to the case-patients according to age, gender, and cancer site.Stroke was related with previous arterial FDG foci when detected on the aortic arch (stroke patients, 86% vs. Controls, 31%; P = 0.03) and especially on carotid bifurcations (stroke patients, 71% vs. Controls, 6%; P = 0.006); and among the 5 case-patients with stroke from carotid territory, 4 (80%) had FDG foci on ipsilateral carotid bifurcations.This pilot study shows that the previous detection of FDG foci on aortic arch and especially on carotid bifurcations of cancer patients is associated with the risk of subsequent ischemic stroke. A further confirmation on larger populations is needed.
Liver Tuberculoma Detected By Ga-67 Imaging
Authors: HITOYA OHTA, TAKAYUKI FUKUYAMA, MADOKA SAKAMOTO, TOMOO KOMIBUCHI, MASAYUKI SHINTAKU
Journal: Clinical Nuclear Medicine (1996)

The authors report a rare case of liver tuberculoma successfully detected by Ga-67 scintigraphy. The patient was referred because of high fever and massive ascites of unknown etiology. Ga-67 scintigraphy showed two hot spots in the liver. Percutaneous biopsy was subsequently performed with ultrasonic guidance, which revealed liver tuberculoma.
THU0189 Patterns of Disease Remission among Patients with Rheumatoid Arthritis Treated with Biologic Therapies in the United States
Authors: S. Narayanan, Y. Lu, R. Hutchings, A. Baskett
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Disease remission is a key target while managing patients with Rheumatoid Arthritis (RA). <h3>Objectives</h3> To assess the patterns of disease remission among Rheumatoid Arthritis (RA) patients recently treated with biologic therapies in the United States. <h3>Methods</h3> A multi-center medical chart-review study of RA patients was conducted among physicians (majority: rheumatologists) in hospitals/private practices to collect de-identified data on patients who are currently on a biologic or recently discontinued a biologic within past 3-months. Physicians were screened for practice-duration and patient-volume and recruited from a large panel to be geographically representative of the US. Patient charts of ∼10 successive patients visiting each center/practice during study period were selected. Physicians abstracted patient diagnosis, treatment patterns/dynamics and patient symptomatology/disease status (incl. assessment of "disease remission", per physician clinical judgment). This retrospective de-identified data collection method met the criteria for IRB exemption under federal regulation 45 CFR 46.101(b)(4). <h3>Results</h3> In 4Q2011, 109 physicians abstracted 851 eligible RA patient charts; patient mean age: 51yrs, female: 73%; 69% and 24% were on 1st line and 2nd line biologic respectively. Overall, 48% of patients were in remission. Remission rate differed by biologic lines: 1st line: 51%, 2nd line: 45%, 3rd line: 26%, 4th line: 27%. Among those with lab measures, results differed between those in remission vs. those who were not: mean ESR (mm/h): 18.9 vs. 37.2, mean CRP (mg/dl): 1.6 vs. 5.3, Rheumatoid Factor (% positive): 87% vs. 89%, Anti-CCP (% positive): 74% vs. 79% and HLA-B27 (% positive): 7% vs. 9%. Among those with data, recent (mean) disease severity scores differed between those in remission vs. those who were not: Tender Joint Count: 1.6 vs. 6.3, Swollen Joint Count: 0.8 vs. 4.8, 100mm VAS score: 18.9 vs. 39.8, HAQ: 0.6 vs. 1.5 and DAS28: 2.2 vs. 4.1. <h3>Conclusions</h3> More than half of the patients were not in remission in this cohort of RA patients in the US who were treated with a biologic recently; they experienced disproportionate level of disease burden. As the line of treatment increased, proportion of individuals achieving remission decreased. These observed patterns warrant further scrutiny to determine the best practices and improve remission rates, thereby alleviating patient burden. <h3>Disclosure of Interest</h3> : None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4793
Totally stapled gastrojejunal anastomosis using hybrid NOTES – single 12 mm trocar approach in acute and survival porcine models
Authors: L Polese, S Merigliano, B Mungo, R Rizzato, R Luisetto, L Norberto
Journal: Endoscopy (2012)

Objective of the study: The aim of this study was to evaluate the feasibility and safety of a totally stapled gastrojejunal anastomosis performed with hybrid NOTES – single 12-mm trans-abdominal trocar approach in a porcine model.
Case 7-2011
Authors: J. Carl Pallais, Bonnie T. Mackool, Martha Bishop Pitman
Journal: New England Journal of Medicine (2011)

A 52-year-old man had low oxygen saturation by pulse oximetry, without other evidence of hypoxemia. He had dermatitis herpetiformis, treated with dapsone, and type 1 diabetes mellitus, with normal hemoglobin A1c levels despite poor glycemic control. A diagnostic test was performed.
Human Hemopoietic Progenitor Cell Toll-like and Thymic Stromal Lymphopoietin Receptor Expression and Function in Allergic Asthmatic Subjects
Authors: Damian Tworek, Delia Heroux, Seamus N. O'Byrne, Paul M. O'Byrne, Judah A. Denburg
Journal: Journal of Allergy and Clinical Immunology (2016)
MPTH-06BRAFV600E MUTATION IN HIGH GRADE GLIOMAS OF CHILDREN AND YOUNG ADULTS
Authors: Sridhar Epari, Hetakshi Kurani, Tejpal Gupta, Girish Chinnaswamy, Jayant Sastri Goda, Prakash Shetty, Aliasgar Moiyadi, Rakesh Jalali
Journal: Neuro-Oncology (2015)

INTRODUCTION: BRAF gene is one of the key genes altered in most paediatric low-grade gliomas, which includes - BRAF fusion and BRAFV600E mutation. In this study, different spectrum of high grade non-ependymal glial tumors of children and young adults, were evaluated for BRAFV600E mutations. MATERIALS AND METHODS: All high grade non-ependymal glial tumours diagnosed at our Institute in patients aged ≤ 30 years during the period of 2011-2013 with available formalin fixed and paraffin embedded (FFPE) tissues were evaluated for BRAFV600E mutation by Sanger sequencing. RESULTS: 67 cases formed the study sample, with male to female ratio was: 2.2:1. Most common location was cerebral hemisphere (n: 57; 85%), followed by posterior fossa (n: 5 cases, which included 3 cases in cerebellum and 2 in cerebellar peduncle); pineal/3rd ventricular:1 and spine:2. Location was not known in two. Histological spectrum included - GBM (n:46; including GBM-O:6; age group - 1-6yrs:4; 7-14yrs:17; 15-20yrs:6 & 21-30yrs:19), anaplastic astrocytoma (aA;n:4; age group - 7-14yrs:1; 15-20yrs:1;21-30yrs:2), anaplastic oligoastrocytoma aOA;(n:6, age group - 7-14yrs:1 & 21-30yrs:5), anaplastic oligodendroglioma (aO; n:8, age group - 18-20yrs:3 & 21-30yrs:5) and pleomorphic xanthoastrocytoma with anaplasia (aPXA;n:3, age group - 15-20yrs:2 & 21-30yrs:1). Only 39 cases (58%) yielded interpretable results for BRAFV600E mutation, of which 5 (12.8%) showed T1799A point mutation (BRAFV600E). Two cases each were of GBM (2/24; 8.3%) and aPXA (2/2; 100%) respectively and one was aO (1/6;16.7%). None of the cases aA (0/4) and aOA (0/3) showed mutation. CONCLUSIONS: 5 cases (12.8%) of pediatric and young adult high grade gliomas showed the BRAFV600E mutation. This study re-emphasizes the occurrence of BRAFV600E mutations in high grade gliomas including oligodendrogliomas and GBMs, raising a possibility of occurrence of distinct low grade-like molecular subset within the histological unequivocally high grade glial tumours.
AB1220 Neonatal lupus erythematosus and isolated congenital heart block
Authors: Ž. Mustapic, I. Malcic, M. Frkovic, D. Dilber, M. Jelusic
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Isolated CHB is a rare condition (1 case in every 15,000 to 20,000 live births) affecting otherwise structurally normal fetal heart. Most of the mothers of affected children are asymptomatic during the pregnancy, but it has been confirmed that there is a firm association with rheumatic diseases (e.g. SLE or Sjögren’s syndrome), and especially anti-SSA/Ro or anti-SSB/La antibodies which are necessary for making a neonatal lupus syndrome diagnosis. These cross the placenta and affect fetal tissues producing rash, cytopenias or liver disorders. The complete heart block is the only irreversible complication. <h3>Methods</h3> We evaluated data from population based Croatian congenital heart disease registry. Data were collected from four major tertiary centers in a five year period <h3>Results</h3> There were 205,051 live births in Croatia, 1,480 of which were patients diagnosed with congenital heart disease, accounting for 0.72% of the live-born children. An overall incidence of 1% of isolated heart blocks among all of the CHDs (overall 3-4 cases per year). Out of these, 30% (5 patients) are born to mothers that suffer from some kind of an autoimmune rheumatic disease. Mothers had been mostly asymptomatic or suffered occasionaly from photosensitive rashes, arthralgias or elevated erythrocyte sedimentation rates. Four of them were diagnosed with a rheumatic disease within a short period of time following the pregnancy (two SLE,one with mixed connective tissue disease and one with leucocytoclastic vascultis). Only one of them had elevated SSA/Ro or anti-SSB/La antibodies. In children initial symptom was bradycardia with subsequent ecohcardiography showing no structural anomalies. The patients (oldest 6 years old) still don’t require PM implantation. One of them developed subsequent restrictive cardiomyopathy (whose mother was treated with dexamethasone at 32 wk of gestation) at the age of 4 months. <h3>Conclusions</h3> Complete isolated congenital heart block is an extremely rare entity. Still, it is of great importance due to a high mortality rate (up to 30%). Therefore, it is mandatory to elucidate its pathogensis and link to maternal rheumatic diseases. The term, neonatal lupus syndrome is commonly used, but there are still no clear-cut criteria pertaining to it. The name of the syndrome itself doesn’t reflect the true nature of the disorder either. <h3>Disclosure of Interest</h3> None Declared
AB1323 Trends in incidence and prevalence of rheumatoid arthritis in a russian population
Authors: E. Myasoedova, S. Myasoedova, N. Musatova
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The incidence of rheumatoid arthritis (RA) has been shown to vary with geographical location and to change with time. RA epidemiology has been widely studied in Western populations. Longitudinal trends in RA incidence and prevalence in Russian population remain largely unexplored. <h3>Objectives</h3> To study the trends in incidence and prevalence of RA in a population of Russia during the 1994–2010 period. <h3>Methods</h3> The data on incident cases of RA in the population of Ivanovo Region (Central Federal District of Russia) during 1994–2010 period were abstracted from the database of Medical Analytical Center of the Department of Healthcare of Ivanovo Region. This comprehensive database includes information on disease occurrence based on annual report of all practicing physicians of Ivanovo region. The diagnosis of RA was based on the fulfillment of the 1987 ACR criteria for RA. Regression models were used to analyze trends in RA incidence in the study population. <h3>Results</h3> During the period from 1/1/1994 to 31/12/2010 a total of 3,283 incident RA cases were registered in the database. Figure shows trends in incidence of RA during the study period. In 1994 the incidence rate was 16.7 (95%CI 16.5–16.9) per 100,000 population. There was a significant increase in RA incidence from 1994 to 2010 with RA incidence rate of 22.6 (95%CI 22.3–22.9) per 100,000 population in 2010 (p-value for trend 0.005). There were no statistically significant changes in RA prevalence (0.50%; 95%0.30–0.57 and 0.55%; 95%CI 0.47–0.62, respectively; p=0.30). <h3>Conclusions</h3> Our study shows an increase in incidence of RA during the 1994–2010 time period in a Russian population, but no apparent change in RA prevalence during this time. More studies are needed to explore the nature of these trends. <h3>Disclosure of Interest</h3> None Declared
Abstract B26: Lack of cyclin D1 kinase activity leads to reduced levels of ErbB2-induced senescence and activation of the Wnt signaling pathway in the mammary gland
Authors: Nelson E. Brown, Charlotte Kuperwasser, Philip W. Hinds
Journal: Cancer Research (2009)

Abstract Proper orchestration of the Oncogene-induced Senescence (OIS) checkpoint relies upon the activation of the ARF-p53 and/or the p16 INK4a-pRB tumor suppressor pathways. Overexpression of cyclin D1, as observed in a large proportion of human breast cancers, is believed to confer proliferative advantage through pRb inactivation. The aim of the current study is to assess the role of cyclin D1-associated kinase activity during ErbB2-induced senescence in the mammary epithelium using a kinase-deficient cyclin D1 knock-in mouse (cyclin D1KE/KE) as a model. The effect of cyclin D1WT/WT or cyclin D1KE/KE on MMTV-ErbB2-induced senescence was analyzed in mammary glands of double-transgenic animals. Surprisingly, we observed that cyclin D1KE/KE/MMTV-ErbB2 mammary glands fail to upregulate markers of cellular senescence when compared to preneoplastic cyclin D1WT/WT/ MMTV-ErbB2 mammary glands. These findings correlated with high levels of proliferation and increased cellularity per duct section despite the fact that the cyclin D1KE/KE/MMTV-ErbB2 mammary epithelial cells do not progress to tumor formation. Moreover, this ectopic proliferation was not accompanied by phosphorylation at Ser780 of pRb, suggesting that other D-cyclins were unable to compensate for the lack of cyclin D1-associated kinase activity. Interestingly, failure to implement the senescence program in mutant mammary epithelium was accompanied by an activation of the Wnt signaling pathway that led to stabilization of beta-catenin in mutant mammary epithelial cells, an observation that others have found to correlate with senescence bypass in MEFs. In addition, induction of senescence in cyclin D1WT/WT/ MMTV-ErbB2 mammary glands did not lead to the accumulation of the gene products encoded by the INK4a/ARF locus. Instead, p18 INK4c expression was increased in D1WT/WT/ MMTV-ErbB2 mammary glands above the basal levels observed in cyclin D1WT/WT mammary tissue that did not express MMTV-ErbB2. A comparable induction was not observed in cyclin D1KE/KE/ MMTV-ErbB2 mammary glands. Taken together, our results suggest a dual role for cyclin D1-associated kinase activity in ErbB2-induced senescence and tumor formation. Cyclin D1 kinase activity is necessary for the full transformation of ErbB2-expressing cells, but it may also play an active role in the cascade that leads ErbB2-overexpressing cells to senesce such that cells that are kinase-deficient can still proliferate due to Wnt signaling activation. Citation Information: Cancer Res 2009;69(23 Suppl):B26.
P50 * DEVELOPING A PHYSIOLOGICALLY RELEVANT BLOOD BRAIN BARRIER MODEL FOR THE STUDY OF DRUG DISPOSITION IN GLIOMA
Authors: S. Kumar, L. Shaw, C. Lawrence, R. Lea, J. Alder
Journal: Neuro-Oncology (2014)

INTRODUCTION: Many in vitro blood brain barrier (BBB) models have been designed to study the underlying mechanism involved in drug transport across the BBB, but most have not included glioma or cells of human origin. Trans-endothelial electrical resistance (TEER) is a key measure of membrane potential and resistance and can be used to quantify the tightness of the BBB. The aim of this study was to evaluate the integrity of various models of the in vitro BBB as determined by TEER measurements. METHOD: Varying combinations of co-culture models were set up using primary cells of human origin which included human macrovascular endothelial cells (HBMEC), human brain vascular pericytes (HBVP), human astrocytes (HA) and human glioma. All cells were grown as monolayers to 80 % confluency in respective media supplemented with 5 % foetal bovine serum and incubated at 37°C, 5 % CO2 prior to establishing the co-culture. Culture dishes and inserts were coated with extracellular matrix (poly-L-lysine or fibronectin). The TEER was measured across the insert for 15 days. RESULTS: A steady increase in TEER was exhibited by all the models up to day 10. The highest TEER value of 250 Ω was recorded for the tri-culture comprising of HBMEC on the insert, HBVP cells in contact and HA cells out of contact of the well. The lowest TEER value of 100 Ω was recorded for the monolayer culture of HBMEC and the co-culture model (HBMEC and HBVP cells). CONCLUSION: The TEER values suggested that the tri culture model showed the highest membrane potential. This model would further be tested by various permeability assays to assess its functionality as an in vitro BBB model.
Abstract 50: Anchored multiplex PCR for detection of gene rearrangements and mutations using next-generation sequencing.
Authors: Zongli Zheng, Boryana Zhelyazkova, Divya Panditi, Hayley Robinson, Jeffrey A. Engelman, John A. Iafrate, Long Le
Journal: Cancer Research (2013)

Abstract We describe a rapid targeted RNA-seq library construction method for the detection of gene fusions in cancer using next generation sequencing. The method involves ligation of half-functional sequencing adaptors to double strand cDNA, followed by two successive PCR reactions using hemi-nested gene-specific 3’ primers with the 5’ adaptor primer, and generates libraries ready for bi-directional sequencing. RNA extracted from formalin-fixed paraffin-embedded biopsies can be used directly without the need to deplete ribosomal RNA, non-target mRNA or genomic DNA. Importantly, the assay can be performed without the need to know the 5’ partner in a gene fusion event. The method can also be applied to genomic DNA for detection of gene mutations, insertions, deletions and copy number changes. The method is very flexible and can allow multiplexing of over 500 targets. We have designed a lung cancer translocation panel which includes 18 amplicons for detections of gene rearrangements involving ALK, ROS1 and RET. For targeted genomic DNA-seq, we have designed a small 96-amplicon panel including tumor hotspots and entire coding sequences of key tumor suppressor genes TP53 and PTEN, and a 623-amplicon panel for 370 exons of 18 tumor suppressor genes. Unlike conventional PCR-based method, this method generates libraries with random sequencing start sites and allows for direct sequencing without having to spiking in controls (e.g. PhiX) on those platforms where high starting site complexity is required, such as the Illumina Miseq sequencer. The assays generally achieve high on-target specificity (&amp;gt;90%), and are very cost-effective. The targeted RNA-seq assay has detected various gene rearrangements in known FISH-positive cases with previously unknown 5’ partners using our archived samples. The small 96-amplicon genomic DNA assay showed 100% of targeted bases having &amp;gt;100-fold coverage and 99.9% having &amp;gt;500-fold coverage with minimal optimization. In our first attempt of targeting 370 exons (626 amplicons, target size 52.3 kilobases), this assay achieved 97% of targeted bases having &amp;gt;100-fold coverage and 93% having &amp;gt;500-fold using ¼ of a Miseq sequencing run. Citation Format: Zongli Zheng, Boryana Zhelyazkova, Divya Panditi, Hayley Robinson, Jeffrey A. Engelman, John A. Iafrate, Long Le. Anchored multiplex PCR for detection of gene rearrangements and mutations using next-generation sequencing. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 50. doi:10.1158/1538-7445.AM2013-50