tbuckley/cabot-search huggingface dataset:
Abstract 1976: Systematic identification of protein-protein interactions by Proximity Ligation Assay
Authors: Tzu-Chi Chen, Kuan-Ting Lin, Yu-Lun Kuo, Pei-Ying Lee, Chun-Houh Chen, Yu-Wen Liu, Chi-Ying Huang
Journal: Cancer Research (2011)

Signal transduction pathways at cellular level require massive protein-protein interactions (PPIs) in response to different environmental cues. Diverse experimental techniques for detecting PPIs have been developed; however, the large amount of data accumulated from various sources has posed a grand challenge in data reliability for PPI. To evaluate the role of PPIs within signaling pathways, we have systematic profiled PPI residing in 28 KEGG pathways, for example mTOR, MAPK, Wnt, VEGF, and Hedgehog, and 3 cancer-related pathways stored in KEGG (http://www.genome.jp/kegg/). Of ∼60000 human PPI collected, 2786 PPI can be detected within targeted 14 pathways. To validate these PPIs, we used a newly developed Proximity Ligation Assay, which detects endogenous PPI through a pair of antibodies that bind to proteins in close proximity. Each PPI can be made visible through DNA amplification and quantified by counting fluorescent dots in the cell. Of 1,204 PPIs examined, 557 PPIs can be confirmed in HeLa cells. Specifically, 383 out of 557 validated PPIs residing within pathways in cancer and mTOR, Wnt and MAPK pathways are considered novel “links” based on annotations of KEGG. Moreover, 79 validated PPIs are cross-talk events among 35 signaling transduction and cancer related pathways. Surprisingly, only 16 PPIs (out of 557 confirmed PPIs) are highly correlated in 7 sets of cancer cells from NCI60 microarray dataset. In this large scale PPI profiling, 41 apoptosis related PPIs were further tested in 3 cancer cell lines to explore the possibility for drug screening. These systematic analyses of endogenous PPI pairs within and between pathways may set the foundation to disrupt the signaling pathways by targeting PPIs in cancer drug discovery.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1976. doi:10.1158/1538-7445.AM2011-1976
Systemic Anticoagulation And Intraocular Hemorrhage
Authors: Gilbert W. Cleasby
Journal: JAMA (1972)

<h3>To the Editor.—</h3> Severe intraocular hemorrhage is an infrequent but well-known complication of disciform macular degeneration (exudative senile maculopathy). In my experience, this has been seen in the absence of anticoagulation therapy. In the case reported, it is possible there could have been some causeand-effect relationship between anticoagulation and intraocular hemorrhage, but it is also quite possible that there was none. It is my opinion that the information presented in this report does not justify the conclusion.
Quantification and Impact of Secondary Osteoarthritis in Patients With Anti–Citrullinated Protein Antibody–Positive Rheumatoid Arthritis
Authors: Camille P. Figueiredo, David Simon, Matthias Englbrecht, Judith Haschka, Arnd Kleyer, Sara Bayat, Axel Hueber, Rosa M. R. Pereira, Juergen Rech, Georg Schett
Journal: Arthritis & Rheumatology (2016)

To search for evidence of secondary osteoarthritis (OA) in patients with rheumatoid arthritis (RA) in a cross-sectional and longitudinal setting, and to relate osteophyte formation to functional outcome.Anti-citrullinated protein antibody (ACPA)-positive RA patients underwent high-resolution peripheral quantitative computed tomography of the hand. Cross-sectional and longitudinal measurements were performed. The number and size (volume) of osteophytes as well as bone erosions were documented. The relationship of osteophytes to bone erosions and to demographic and disease-specific data was evaluated by multiple logistic regression models.A total of 202 ACPA-positive RA patients were enrolled in the cross-sectional part of the study, and a total of 77 ACPA-positive RA patients were enrolled in the longitudinal analysis (interval of 1.5 years between baseline and follow-up assessment). The mean ± SD number of osteophytes per patient was 1.3 ± 2.3, and the mean ± SD osteophyte volume per patient was 2.6 ± 4.9 mm(3) . The total number of erosions was significantly correlated with the total number of osteophytes (P < 0.001), and the total volume of erosions was significantly correlated with the total volume of osteophytes (P < 0.001). Moreover, the number of osteophytes was related to age (P < 0.001) and disease duration (P = 0.001), while the volume of osteophytes was related to age (P = 0.001), disease duration (P < 0.001), and function as measured by the Health Assessment Questionnaire (P = 0.013). Multivariate regression analyses showed an independent association between osteophytes and erosions. In the longitudinal analysis, the mean number (P = 0.033) and volume (P < 0.001) of osteophytes increased significantly in RA patients during their disease course.Age, disease duration, and bone erosions are associated with osteophytes, indicating development of secondary OA in patients with RA.
Percutaneous ethanol injection (PEI) of hepatocellular carcinoma (HCC) in liver cirrhosis: Long term follow up in 72 patients
Authors: I DESIO
Journal: Hepatology (1995)

To evaluate the prevalence and predictors of long-term impairment, epilepsy, mortality, and recurrences after the first stroke in a cohort of Chilean children.A prospective study involving 98 children who suffered a first stroke and underwent follow-up for at least 3 years in a single center. Functional outcome was measured using the modified Rankin Scale for children. We utilized multivariate logistic regression models to estimate the odds ratios (ORs) for outcomes while adjusting for age, sex, and underlying conditions (significance <.05).Stroke recurrences were present in 18 children and were strongly associated with arteriopathies (OR 8.11; CI 1.5-43). Of 26 children who died during the follow-up period, a significant proportion had a cardiopathy (OR 6.57; CI 1.3-32) or a chronic head and neck disease (OR 41.3; CI 3.5-490). Among 72 survivors (median age 1.49 years; 38 girls; mean follow-up time 4.85 years), 28 presented marked impairment; these children were younger (P = .019) and had more commonly arteriopathies (OR 9.33; CI 1.7-51) and epilepsy (OR 10.5; CI 3.1-36) as compared to survivors without disabilities. Cumulative epilepsy prevalence was 55.6%; children with epilepsy were younger (P = .037) and had more commonly acute symptomatic seizures (OR 12.16; CI 2.93-50.4) as compared to survivors without epilepsy.The prevalence of long-term adverse outcomes after childhood stroke is high and does not differ from other geographical and racial groups. Younger age, acute seizures, and arteriopathies but not sex and other underlying conditions predict adverse outcome following childhood stroke.
Perception of Risk Heightens Stress of Breast Cancer Screening
Authors: K. Smigel
Journal: JNCI Journal of the National Cancer Institute (1993)

Journal Article Perception of Risk Heightens Stress of Breast Cancer Screening Get access Kara Smigel Kara Smigel Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 85, Issue 7, 7 April 1993, Pages 525–526, https://doi.org/10.1093/jnci/85.7.525 Published: 07 April 1993
A memorable patient: A promise broken
Authors: A. Savage
Journal: BMJ (2000)

Mrs A was a kindly, middle aged woman with a large house in north London, who had been devoted to her mother, a patient of mine. When her mother died she took in Dr W, who had the spacious ground floor room, his own furniture, and all services. I became his GP.

He was a charming old man with chronic heart failure and we got on well. He volunteered nothing about his past and I never asked …
COMPARISON OF THE PROGNASTIC USEFULNESS OF THALLIUM SCINTIGRAPHY ACCORDING TO THE POPULATION REFERRED FOR TESTING.
Authors: Alan Rozanski
Journal: Clinical Nuclear Medicine (1986)

Jamshid Maddahi, George O'Byrne, Howard Staniloff, Nicholina Said, Daniel S. Berman, Cedars-Sinai Med Ctr., L.A., CA
Abstract 20047: Validation of the Simple Acute Coronary Syndrome (SACS) Score for Identifying Obstructive Coronary Artery Disease
Authors: Daniel Matta, Christina Marsalisi, Wayne Ruppert, Ravi Korabathina
Journal: Circulation (2015)

Background: Each year, up to 136,000 individuals suffering from acute coronary syndrome (ACS) are misdiagnosed and discharged from our nations’ emergency departments. We developed the Simple Acute Coronary Syndrome (SACS) score that tabulates a patient’s symptoms, ECG findings, risk factors, and cardiac markers (Figure 1). Our aim was to validate this novel scoring tool in its ability to identify the presence of obstructive coronary artery disease (CAD). Methods: A single-center retrospective chart review was performed after institutional review board approval. The charts of 42 consecutive patients who presented with ACS and who were treated with an invasive strategy were reviewed. Scores were calculated for each patient using the SACS tool as well as the Modified Thrombolysis in Myocardial Infarction (TIMI) ACS calculator. The study endpoint was the presence of at least one vessel obstructive CAD at cardiac catheterization. Descriptive statistics were employed. Results: The stratification of SACS and Modified TIMI scores for each of the 42 ACS patients is shown in Figure 2. In patients with a SACS score that was less than 3, none of these patients were found to have obstructive CAD at cardiac catheterization. For patients with a SACS score of 4 or higher, 100% of these patients were shown to have obstructive CAD. For patients with Modified TIMI scores of 1 and 2, 3/11 (27%) were found to have obstructive CAD. Conclusions: The novel SACS scoring system identifies ACS patients who will have obstructive CAD more reliably than more traditional scoring systems. The SACS scoring tool needs to be validated in larger scale studies.
Percutaneous Catheter Commissurotomy in Rheumatic Mitral Stenosis
Authors: James E. Lock, M. Khalilullah, Savitri Shrivastava, V. Bahl, John F. Keane
Journal: New England Journal of Medicine (1985)

We attempted percutaneous transcatheter-balloon mitral commissurotomy in eight children and young adults (9 to 23 years of age) with rheumatic mitral stenosis. The atrial septum was traversed by needle puncture, and an 8-mm angioplasty balloon was advanced over a guide wire. The atrial septal perforation was then dilated to allow passage of the valvuloplasty balloon catheter (18 to 25 mm) across the mitral annulus. Inflation of the transmitral balloon decreased the end-diastolic transmitral gradient temporarily in all patients (from 21.2 +/- 4.0 mm Hg [mean +/- S.D.] to 10.1 +/- 5.5 mm Hg; P less than 0.001). The immediate decrease in the gradient was associated with increases in cardiac output (from 3.8 +/- 1.0 to 4.9 +/- 1.3 liters per minute per square meter of body-surface area; P less than 0.01) and in the calculated mitral-valve-area index (from 0.73 +/- 0.29 to 1.34 +/- 0.32 cm2 per square meter; P less than 0.001). Murmur intensity diminished immediately after commissurotomy in all patients. The greatest reduction in pressure gradient (76 to 95 per cent) occurred when the largest balloon (inflated diameter, 25 mm) was used in the smallest patients (0.9 to 1.2 m2). The balloon commissurotomy produced minimal mitral regurgitation in only one child. Follow-up catheterization (at two to eight weeks) demonstrated persistence of hemodynamic improvement with evidence of partial restenosis in one patient. These early results indicate that balloon mitral commissurotomy can be a safe and effective treatment for children and young adults with rheumatic mitral stenosis.
En bloc resection: does it really matter?
Authors: Bas Weusten
Journal: Endoscopy (2014)

Gastrointestinal endoscopy has increasingly become a therapeutic procedure rather than a solely diagnostic one. In the field of early neoplasia of the digestive tract, therapeutic endoscopy started many years ago when polypectomies of pedunculated colonic adenomas became widely practiced. In current practice, precancerous lesions throughout the digestive tract, and even early invasive cancers, are removed endoscopically.
Radial endosonography versus oesophagogastroduodenoscopy for detecting oesophageal and gastric varices
Authors: I Tozzi
Journal: Endoscopy (2011)

Gastrointestinal bleeding from oesophageal and gastric varices may be fatal in as many as one third of patients with liver cirrhosis. Early diagnosis is necessary for prophylaxis and prevention of the first episode of bleeding.
CORRELATION BETWEEN THE SEVERITY OF CORONARY ARTERY STENOSIS AND CORONARY ARTERY CALCIUM, CAROTID ATHEROSCLEROSIS, ARTERIAL STIFFNESS AND LEFT VENTRICULAR MASS INDEX IN METABOLIC SYNDROME PATIENTS
Authors: Jae Hyoung Park, Jae Young Cho, Hyung Joon Joo, Soon Jun Hong, Cheol Woong Yu, Do-Sun Lim
Journal: Journal of the American College of Cardiology (2015)
Retention of Iodine-131 in a Thoracic Esophageal Diverticulum Mimicking Metastatic Thyroid Cancer
Authors: HO-CHUN SONG, SEONG-MIN KIM, YOUNG-JUN HEO, HEE-SEUNG BOM
Journal: Clinical Nuclear Medicine (2002)

SONG, HO-CHUN M.D., Ph.D.; KIM, SEONG-MIN M.D.; HEO, YOUNG-JUN M.D.; BOM, HEE-SEUNG M.D., Ph.D. Author Information
The importance of updating the family history of breast cancer survivors.
Authors: L Madlensky, L Wasserman, BA Parker, JP Pierce
Journal: Cancer Research (2009)

Abstract Abstract #1097 BACKGROUND: The cancer family history is the most important tool in identifying women who may be at high risk of having the hereditary breast-ovarian cancer syndrome (HBOCS) caused by mutations in the BRCA1 and BRCA2 genes. While many breast cancer patients may be asked about their family history at the time of their initial diagnosis, it is not known how many women initially classified as low risk of HBOCS become high risk of HBOCS over time, as new cancers are diagnosed in their relatives.&amp;#x2028; PURPOSE: 1)To determine how the prevalence of "high risk" cancer family histories changes over time in cancer patients, and; 2) To determine how many breast cancer survivors at high risk for HBOCS self-report having had genetic testing for BRCA1/2.&amp;#x2028; METHODS: Breast cancer survivors in the Women's Healthy Eating and Living (WHEL) study provided detailed family history data at baseline and again at study closure. The Myriad prevalence tables were used to categorize survivors as "high risk" of HBOCS if their risk of having a mutation in BRCA1/2 was 10% or greater.&amp;#x2028; RESULTS: At baseline, 8.2% of 2508 survivors were classified as high risk. At follow-up (avg. 7.5 years later), an additional 5.8% of women became high risk due to new cancers in their families. Among high-risk survivors, 15% reported that they had never heard of BRCA testing. Of the 85% who had heard of testing, 23% reported that they had been tested.&amp;#x2028; CONCLUSIONS: The prevalence of "high risk" cancer family histories increased by 70% in a cohort of breast cancer survivors over 7.5 years. It is important for clinicians to identify high-risk families not only at initial diagnosis, but to update the family history periodically. A substantial proportion of high-risk women had never heard of BRCA1/2 testing, and could benefit from genetic counseling to discuss the cancer risk in their families. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1097.
Abstract PD07-09: Hypoxia (<i>VEGF-S)</i> Signature and <i>CRYAB</i> Predict Response to Neoadjuvant Anthracycline/Taxane Containing Chemotherapy within Triple Negative and Unselected Breast Tumors
Authors: MCU Cheang, JS Parker, X He, W Zhao, C Fan, A Prat, L Carey, Investigators I-SPY, CM. Perou
Journal: Cancer Research (2010)

Abstract Background: Aggressive breast tumors are a clinically challenge to manage. A VEGF-signature (VEGF-s) developed in vivo (Hu et al. BMC Med. 09) and the profile of the anti-apoptotic gene and regulator of tumor angiogenesis, CRYAB (Moyano et al. J Clin Invest 06; Dimberg et al. Blood 08), are both associated with distant metastasis. In this study, we sought to test if these two mechanistically related biomarkers predict pathological complete response(pCR) to anthracyline/taxane-based neoadjuvant chemotherapy. Methods: Gene expression data of pre-treatment fresh frozen tumors were combined, 149 from the ISPY trial (Agilent 44K) and 225 from MD Anderson Cancer Center(MDACC) (Affymetrix HG U133A). Patients from I-SPY were treated with doxorubicin/cyclophosphamide followed by a taxane. Patients from MDACC were treated with paclitaxel followed by sequential fluorouracil-AC. Tumors were classified into intrinsic subtypes using PAM50(Parker et al. J Clin Oncol 09) and Claudin-low(CL) predictor(Prat et al., Submitted). Odds ratios of biomarkers to pCR were determined using logistic regression. Predictive values of multivariable models were estimated using receiver operating characteristic curve. Results: 319 patients had complete data; the pCR rate was similar between the datasets (ISPY 22%, MDACC 20%). Both the VEGF-s and CRYAB were highly expressed within Basal-like(BL) and CL (P&amp;lt;0.0001); these two biomarkers were positively correlated with each other (r: 0.4, P&amp;lt;0.001). In univariate analysis, both VEGF-s (P&amp;lt;0.001) and CRYAB (P&amp;lt;0.001) were significantly associated with pCR. A multivariable test including tumor size, grade, ER, Her2, intrinsic subtypes, VEGF-s and CRYAB, had an AUC value of 0.86 to predict pCR(Table 1). T stage, Her2, intrinsic subtypes and CRYABwere significant. 82 patients with triple-negative tumors within this combined cohort. In univariate analysis, T stage, grade or classifying tumors into BL vs not did not predict pCR; but VEGF-s (p= 0.04) and CRYAB (p = 0.02) were associated with pCR. Multivariable model, including VEGF-s, CRYAB and the clinical variables, had a predictive value of 0.71 to pCR(Table 1), which identified CRYAB as the most significant factor by likelihood ratio test. Table 1: Multivariable logistic regression models to predict pCR. Conclusion: VEGF-s and CRYAB expression are associated with aggressive subtypes and may possibly help to predict response of anthracycline/taxane-based neoadjuvant chemotherapy beyond ER, Her2, and intrinsic subtypes. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr PD07-09.
THU0557 Nailfold Videocapillaroscopic Abnormalities and Selected Angiogenesis Modulators in Patients with Raynaud's Phenomenon after Multiwave Locked System (MLS) Laser Therapy
Authors: A. Kuryliszyn-Moskal, J. Kita, A. Dakowicz, S. Chwieśko-Minarowska, D. Moskal, B. Kosztył a-Hojna, E. Jabł ońska, P.A. Klimiuk
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Raynaud9s phenomenon (RP) is a vascular disorder characterised by reversible vasospastic response of the fingers and toes to cold or emotional stimuli. It can be primary (idiopathic) or secondary (Raynaud9s syndrome), related to a number of different pathological conditions, including rheumatic diseases. <h3>Objectives</h3> The aim of this study was to investigate the influence of the Multiwave Locked System (MLS) laser therapy on clinical features, microvascular changes in nailfold videocapillaroscopy (NVC) and circulating modulators releasing as a consequence of vascular endothelium injury such as vascular endothelial growth factor (VEGF), angiopoietin 2 (Ang-2) and nitrogen oxide (NO) in patients with primary and secondary RP. <h3>Methods</h3> Seventy-eight RP patients and 30 healthy subjects were recruited into the study. All patients with RP received MLS laser irradiation for 3 weeks. Clinical, NVC and laboratory investigations were performed before and after MLS laser therapy. The serum concentration of VEGF and Ang-2 were determined by an enzyme-linked immunosorbent assay (ELISA). Total NO concentration was determined using an indirect method based on assessment of nitrite concentration according to Griess9s reaction. <h3>Results</h3> After three weeks of MLS laser therapy the clinical improvement manifested by decreasing of the number of RP attacks, duration of single attack and pain intensity in RP patients was observed. After MLS laser therapy, in 65% of patients with primary and in 35% with secondary RP an increase in the loop number and/or a reduction of avascular areas in NVC were observed. In comparison with a control group, higher serum concentration of VEGF, Ang-2 and NO in RP patients was demonstrated (in all cases p&lt;0.001). After MLS laser therapy a reduction of Ang-2 and NO levels in secondary RP patients (in both cases p&lt;0.001) and of Ang-2 in primary RP patients (p&lt;0.01) was found. <h3>Conclusions</h3> Our results suggest that NVC may reflect microvascular changes associated with clinical improvement after MLS laser therapy in patients with primary and secondary RP. Ang-2 and NO serum levels may be a useful marker of microvascular abnormalities in RP patients treated with MLS laser therapy. <h3>Disclosure of Interest</h3> : None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.1657
A3.18 Comparison and evaluation of different anti-DSDNA antibody detection methodologies in a cohort of Hungarian patients
Authors: E Nagy, P Gergely, E Kiss, J de Jong, D Hamann, R Rasonyi, I Kenessey, Gy Poór
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Introduction</h3> Anti-double stranded DNA (dsDNA) antibodies are useful for the diagnosis of systemic lupus erythematosus (SLE). However, these antibodies are very heterogeneous and low avidity anti-dsDNA antibodies may occur in other rheumatic diseases as well. As a consequence of their heterogeneity, detection of anti-dsDNA antibodies partly depends on the type of assay used. <h3>Aim of the study</h3> To compare five different anti-dsDNA antibody assays with the current one used in our routine diagnostics in order to set up an algorithm for better differentiation of high avidity anti-dsDNA antibodies from low avidity ones. <h3>Methods</h3> From sera of 1446 consecutive patients sent to our laboratory for anti-dsDNA antibody measurements during a 4 months period, all sera that were found positive with our assay (ELISA1) were included in the study: 92 samples from SLE, 62 from other connective tissue disease (CTD) and 36 from patients with other inflammatory diseases (190 in total). All selected sera were tested for anti-dsDNA antibodies with two additional enzyme-linked immunoassays (ELISA2 and a high avidity ELISA3), with two Crithidia luciliae immunofluorescence tests (CLITF1 and CLITF2), and with Farr method used as the gold standard. Sera of 20 healthy blood donors were used as control. <h3>Results</h3> In the selected group, out of the six methods the Farr, CLIFT1 and CLIFT2 had the highest specificity (93.87%, 90.81% and 81.63%) but lowest sensitivity (30.43%, 31.52% and 39.13%) for SLE. ELISA2 had a lower specificity (38.77%), but still high sensitivity (92.39%). The highest agreement by the Cohen’s Kappa was seen between CLIFT1 and CLIFT2 (0.558), Crithidia tests and Farr (0.531 and 0.489) and ELISA2 and ELISA3 (0.472). All Farr positive samples (n = 23) were also positive in ELISA’s. The levels of anti-dsDNA antibodies detected with ELISA2 and ELISA3 correlated significantly (Spearman rank correlation coefficient Rho: 0.755; p&lt;0.05). All other quantitative methods had a lower correlation coefficient. <h3>Conclusions</h3> Based on these results, a two-step algorithm is suggested for the detection of anti-dsDNA antibodies: after screening samples with an ELISA test as the primary method (preferably ELISA2), CLIFT1 should be used to confirm positive results obtained in step one.