tbuckley/cabot-search huggingface dataset:
Abstract 4222: CCN5 deficient MCF-7 side population exhibits stem cell like behavior and formed aggressive tumor in xenograft model
Authors: Kakali Dhar, Snigdha Banerjee, Joyce Slusser, Monami Majumder, Sushanta K. Banerjee
Journal: Cancer Research (2010)

Abstract The side population (SP) has been identified in a variety of solid tumor cell lines including human breast cancer cell lines. This population of cells has been considered as cancer stem cells (CSCs). Cancer recurrence is currently thought to be due to non-dividing cancer stem/progenitor cells that are resistant to chemo- and radio-therapies. Different therapeutic approaches need to be considered for the elimination/transformation of the cancer stem cells. To reshape our therapeutic approach to breast cancer, we aimed this study and isolated the SP from MCF-7 breast cancer cell line. SP was confirmed by both positive and negative cell surface molecular markers. These include CD44, CD24, b-catenin, E-cadherin, Keratin 19, Oct-4, Notch-1 and Vimentin. Our studies showed that SP and non-SP exhibit differential expression of CCN5, an anti-invasive gene. CCN5 expression was undetected or barely expressed in SP. The comparative studies showed that the in vitro migratory property of SP was significantly higher as compared to non-SP. Moreover, the introduction of CCN5 in SP may enhance the epithelial properties as evidenced by differential expression of MET (mesenchymal-epithelial transition) molecular markers. SP shows higher tumorigenicity in immunodeficient mice. These findings indicate that CCN5 deficiency could be an essential molecular event for the development of a side population having invasive phenotype. Therefore, CCN5 can be used as a therapeutic reagent for advanced breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4222.
Abstract 2589: Sulfated hyaluronic acid: A possible targeted therapy for prostate cancer
Authors: Travis Yates, Anaid Benitez, Luis E. Lopez, Vinata B. Lokeshwar
Journal: Cancer Research (2011)

Abstract Tumor cell-derived hyaluronidase HYAL1, which degrades hyaluronic acid (HA), promotes tumor growth and metastasis. In prostate cancer (CaP), HYAL1 expression in biopsy and prostatectomy specimens is an independent prognostic marker for predicting disease progression. Sulfated hyaluronic acid (sHA) inhibits HYAL1 activity through a mixed inhibition mechanism. Antitumor activity of sHA has not been evaluated. In this study we evaluated effects of sHA on CaP cells. The effect of sHA (0-40 ug/ml) on cell proliferation and apoptosis was examined in CaP cells (PC3-ML, LNCaP, LNAI, C4-2B, LAPC-4, DU145, RWPE1) by cell counting and Cell Death ELISA kit. Matrigel invasion and Boyden chamber assays were used to test the effect of sHA on invasive activity. Effect of sHA on signaling, apoptosis cascade, androgen receptor activity, VEGF, NFkB activity, and HA receptor levels, was evaluated by immunoblotting, Q-PCR and reporter assays. Athymic mice bearing LNAI xenografts were treated with sHA (25 and 50 mg/kg) by i.p. injection. sHA inhibited proliferation, motility and invasion in CaP cells. At IC50 for HAase activity inhibition (∼ 10-μg/ml), sHA induced &amp;gt; 3-fold apoptosis and inhibited invasive activity of CaP cells, which could be partially reversed by HA-fragments. sHA induced caspase-3, -8, -9 activation, up-regulation of Fas, Fas-L, FADD, and DR4 and down regulated bcl-2, phospho-bad, bcl-XL, phospho-Akt, phospho-IkB, and phospho-AR. At IC50, sHA caused &amp;gt; 90% inhibition of NFKB-reporter activity, PSA-promoter activity and transcriptionally down regulated HA-receptors (CD44 and RHAMM) and VEGF. Effect of sHA could be reversed by overexpression of m-Akt. Down regulation of CD44 and RHAMM, together with sHA synergistically inhibited cell proliferation and Akt activation. sHA significantly inhibited LNAI tumor growth. The majority of the animals did not form palpable tumors at 50-mg/kg dose even after 70 days when the treatment was stopped on 42nd day. No weight loss or serum and organ toxicity was observed in sHA treated animals. Tumors showed reduced microvessel density (∼3-fold) and increased TUNEL positive cells (&amp;gt; 5-fold). This is the first study that shows targeting HYAL1 (by sHA – a novel non-toxic agent), may control CaP growth and progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2589. doi:10.1158/1538-7445.AM2011-2589
A TENTATIVE NEW RISK SCORE FOR ST-SEGMENT ELEVATION MYOCARDIAL INFARCTION TREATED WITH PRIMARY PERCUTANEOUS CORONARY INTERVENTION: A TOOL FOR RISK ASSESSMENT AT PRESENTATION
Authors: Istvan Hizoh, Zalan Gulyas, Gabor Szabo, Zsuzsanna Majoros, Istvan Zatroch, Laszlo Major, Andras Korda, Gabor Kerecsen, Robert Gabor Kiss
Journal: Journal of the American College of Cardiology (2012)
PMO-208 Double balloon enteroscopy in a district general hospital: the experience so far
Authors: C Parker, R Perowne, D Nylander, S Panter
Journal: Gut (2012)

<h3>Introduction</h3> Double Balloon Enteroscopy (DBE) is widely used in clinical practice worldwide and can be used to explore the small bowel in an antegrade or retrograde approach. A DBE service at South Tyneside District Hospital was commenced in January 2010 to complement the existing capsule endoscopy service. <h3>Methods</h3> Patient records were examined retrospectively for all DBE9s performed at South Tyneside District Hospital between January 2010 and January 2012. Information recorded included: indication for procedure, route of procedure, average depth of insertion, findings of procedure, therapy performed, sedation used and complications. <h3>Results</h3> A total of 37 procedures (17 (46%) in first 12 months, 20 (54%) in 2<sup>nd</sup>) were performed: 17 anal, 1 via ileostomy, 19 oral. 81% were done under sedation using a combination of midazolam and pethidine (average doses 4.7 mg midazolam, 62 μg pethidine), 19% were performed under GA. Average depth of insertion for all procedures was 197 cm for oral DBE and 81 cm for anal DBE. Average depth for year 1: 204 cm oral, 69 cm anal. Average depth of insertion for year 2: 190 cm oral, 93 cm anal. Indications: Suspected mid GI bleeding 30%, definite or suspected Crohns 46%, neoplasia, 10.5%, abnormal imaging 10.5%, symptoms/signs only 3%. See Abstract PMO-208 table 1. DBE Findings: Diagnostic yield: 30% for occult GI bleeding, 46% for suspected or definite Crohns with an overall change in management (or can explain symptoms) in 37%. See Abstract PMO-208 table 2. Overall biopsies were taken in 43% (37.5% year 1, 47% year 2). Therapy was performed in 9% (1 Crohn9s stricture dilatation, 2 Peutz Jegher polypectomies.). There have been no complications. <h3>Conclusion</h3> Our DBE service appears safe. In the second 12 months there were deeper depths of insertion for the anal approach suggesting the “learning curve” is greatest for this approach. Increasing amounts of therapy and tattoos are being performed as experience is increasing. A recently published systematic review of DBE<sup>1</sup> found the main indication for referral for DBE to be GI bleeding (60.2%), interestingly our results show a different picture with the main referral indication being Crohn9s disease (in 46%) and GI bleeding in only 30% of cases. The overall rate of positive findings is lower than in the literature at 37% and may be due to the different referral indications as well as the small number of procedures performed so far. <h3>Competing interests</h3> None declared. <h3>Reference</h3> 1. <b>Xin</b>, <i>et al.</i> Indications, positive findings, total enteroscopy, and complications of DBE. <i>GIE</i> 2011;<b>74</b>:563–70.
Abstract 3359: Salinomycin reduces stem cell properties and induces apoptosis in mammary epithelial cell
Authors: Hyun sook An, Han-Na Kang, Myoung-Hee Kang, Jung-Lim Kim, Jun-Suk Kim, Jea-Hong Seo
Journal: Cancer Research (2012)

Abstract Tumor initiating cells or cancer stem cells (CSCs) were characterized as a unique subpopulation with stem cell features in many types of cancer. Although there has been a number of studies on breast cancer stem cells, inhibitory mechanisms of tumor initiation, progression, and resistance to therapy are unknown. Salinomycin is a chemical isolated from Streptomyces albus and a potassium ionophore. It has been used for many years to control coccidiosis. Recently, salinomycin has been reported to act as selective breast cancer stem cell inhibitor because of its property of reducing the cancer stem cell and inducing the apoptosis in cancer cell. Thus, we investigated the anti-cancer property of salinomycin in mouse mammary epithelial cell. With CDμgeo (mouse mammary epithelial cell), MTT assay was used to confirm cell viability after salinomycin treatment (0, 0.5, 1, 2, 5, 10 μm/ml) upon anchorage-dependent state. We observed that salinomycin reduced the cell proliferation dose-dependently. Also, mammosphere formation assay was performed to observe stem cell-like property after salinomycin treatment in anchorage-independent state. We confirmed salinomycin reduced the number and the size of mammosphere in 72, 144, and 216 hours. We performed salinomycin pre-treatment (0, 0.5, 1, 2, 5 μm/ml) for 12-48 hours upon anchorage-dependent state. Results by western blotting revealed that salinomycin promoted apoptosis of mouse mammary epithelial cells by activating both caspase-3 and -9 and inducing PARP cleavage. To confirm the reduction of the side population level, we performed FACS analysis using CD49+, CD29+, and CD24+ markers. Based on our findings, we concluded that anti-cancer effect of salinomycin reduced stemness and induced apoptosis in mouse mammary epithelial cell. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3359. doi:1538-7445.AM2012-3359
Treatment of unusual rectal foreign body using a Foley catheter
Authors: Abdurrahim Sayılır, Ibrahim Düzgün, Bülent Güvendi
Journal: Endoscopy (2014)

A 70-year-old man presented to our emergency department with a history of abdominal pain and bloating and lack of defecation for 3 days. A round, hard foreign body with a smooth surface was palpated on digital rectal examination. The man admitted having introduced a mandarin into the rectum 4 days previously. An attempt at manual extraction of the foreign body was unsuccessful. Endoscopic examination of the rectum showed that a round, orange-colored mandarin was almost completely obstructing the lumen, and did not allow the colonoscope (Pentax EC-380LKp, Tokyo, Japan) to pass to proximally beyond it ([Fig. 1]). Grasping the mandarin with a 50-mm polypectomy snare (Endo-Technik, Solingen, Germany) failed because of the mandarin's round and smooth surface. A 14-French Foley catheter (PlusMed; Sisco Latex PVT, Chennai, India) was inserted and passed proximally to the mandarin under colonoscopic observation ([Fig. 2]). The balloon of the catheter was inflated with 20 mL water and pulled gently. In this way the mandarin was fully extracted without complication ([Fig. 3]). After the extraction, control endoscopic examination revealed only mild superficial laceration of the rectal mucosa. The patient was observed for a few hours after the procedure and discharged from the hospital on the same day.
Abstract 920: Mislocalization of the polarity protein Scribble promotes tumorigenesis in the mouse mammary gland
Authors: Michael E. Feigin, Avi Z. Rosenberg, Robert D. Cardiff, Senthil K. Muthuswamy
Journal: Cancer Research (2011)

Abstract Recent evidence from our lab has demonstrated that the basolateral polarity protein Scribble is frequently mislocalized in human tumors and can cooperate with ErbB2 and Myc to induce mammary tumors. However, we do not know if mislocalization of Scribble can initiate cell transformation and tumorigenesis. To test the hypothesis that Scr mislocalization can induce tumorigenesis, we generated transgenic mice expressing a Scr point mutant that has been shown to mislocalize to the cytosol, under the control of the MMTV promoter (ScrP305L). ScrP305L mice develop mammary tumors with long latency, suggesting that secondary genetic events are required for tumor initiation. The tumors are histologically diverse, and immunohistochemical and gene expression analysis indicate that they express high levels of basal markers, including cytokeratins 5, 6 and 14, and low levels of the luminal marker E-cadherin. Inspection of the developing virgin mammary gland reveals that Scr mislocalization impairs tertiary branching and ductal density. While primary and secondary ducts appear normal, tertiary branches fail to form, suggesting a defect in luminal differentiation. These changes can be observed as early as eight weeks after birth; however, by one year of age, multifocal hyperplasias are apparent in most mice. We conclude that mislocalization of Scribble in the mouse mammary gland impairs luminal differentiation, resulting in branching defects and, through acquisition of secondary genetic events, tumors of varying histotype. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 920. doi:10.1158/1538-7445.AM2011-920
Bile-Salt Deficiency in the Steatorrhea Following Resection of the Ileum and Proximal Colon
Authors: William G. M. Hardison, Irwin H. Rosenberg
Journal: New England Journal of Medicine (1967)

STEATORRHEA is common in patients who have had all or part of the ileum removed. Originally, investigators assumed that this was because fat absorption occurred mainly in the ileum.1 In 1957, when Borgström et al.2 demonstrated that over 90 per cent of fat was absorbed in the duodenum and proximal jejunum, a different explanation was required.In 1964 Borgström, Lundh and Hofmann3 demonstrated that the ileum was the principal site of bile-salt absorption in man. A year later, Playoust, Lack and Weiner4 showed that steatorrhea produced in dogs by resection of the ileum was accompanied by a greatly shortened sodium . . .
Abstract 802: Minimal deleted regions and their clinical significances in oral squamous cell cacinomas
Authors: Ling-Ling Hsieh, Huei-Tzu Chien, Shiang-Fu Huang, I-How Chen, Chun-Ta Liao, Hung-Ming Wang
Journal: Cancer Research (2010)

Abstract With improvements in technologies, DNA alterations on a genome-wide scale have been successfully evaluated in several human cancers. To determine frequently novel deletion regions in oral squamous cell carcinomas (OSCCs) in Taiwan and regions specifically associated with clinicopathologic parameters and prognoses, the 400 polymorphic microsatellite markers from Applied Biosystems PRISM Linkage Mapping Set-MD10 (PE Biosystems, Foster City, CA) were used to examine 63 male primary tumors. To make sure that tumor tissue samples for genome-wide LOH screening consisted &amp;gt;90% tumor cells, LCM was performed on immunostaining slide using a PixCell laser capture microscope (Arcturus Engineering, Mountain View, CA). For increasing reliability of statistical analysis, only when a microsatellite marker was able to provide LOH information in more than 30% (&amp;gt; 19 cases) of the DNA samples was considered to be a successful allelotyping. Based on this criterion, 276 markers were included for the present study. The mean heterozygosity rate in these 276 microsatellite markers was 70.04%. The percentage of LOH among the markers was ranged from 3.85% (marker D20S171) to 65.52% (marker D18S478) with a mean of 33.63% (SD, 12.28%). Twenty-four markers displayed &amp;gt;50% LOH and chromosome regions at 2p21, 2p11.2, 5p15.1, 5q35.1 and 7q21.13 were novel frequent LOH regions not reported previously in OSCC. In this study, we found that markers with a LOH frequency &amp;gt;35% are likely to show LOHs associated with cancer-specific phenotypes. Therefore, only markers with a LOH frequency &amp;gt;35% (121 markers) were used to examine whether there were associations between LOH and the clinicopathologic parameters, including age, anatomic site, tumor stage and risk factors of oral cancer (cigarette smoking, alcohol drinking and AQ chewing) and construct minimal deleted region (MDR). Thirty-two MDRs were defined and 10 novel MDRs were noted. A loss of MDR c19r2 (19q13.42-19q13.43) was correlated significantly with cigarette smoking and a loss of MDR c14r1 (14q24.2-14q32.12) and c11r1 (11q13.4-11q25) was associated with disease-free survival. A loss of MDR c14r1 and c11r1 was associated with the magnitude of genomic instability estimated by the proportion of loci showing LOH (PLOH) and the degree of chromosomal derangement estimated by the mean fractional allelic loss (FAL) in a tumor. In conclusion, our comprehensive allelotype analyses have unveiled a few MDRs involved in the development of OSCC in Taiwan. These results provide useful clues for identification of putative tumor suppressor genes by fine mapping of the suspected MDRs. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 802.
TARGETED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES FACILITATE ENGRAFTMENT OF THE IPSC-DERIVED CARDIOMYOCYTES INTO THE INJURED MURINE MYOCARDIUM
Authors: Morteza Mahmoudi, Atsushi Tachibana, Jeff Cohen, Andrew Goldstone, Bryan Edwards, Eric Rulifson, Y. Joseph Woo, Phillip Yang
Journal: Journal of the American College of Cardiology (2016)
SP0126 NKT and Related Cells: Key Roles in Spondyloarthritis?
Authors: D. Elewaut
Journal: Annals of the Rheumatic Diseases (2013)

<h3></h3> Natural killer T (NKT) cells are a unique T cell subset with features of both innate and adaptive immunity. Several subsets of NKT cells exist, the most known is characterized by an invariant Valpha14-Jalpha18 T cell receptor alpha chain and a restricted Vbeta repertoire, also called invariant NKT (iNKT) cells. From a functional perspective, iNKT cells are unique given their ability to rapidly produced copious amounts of Th1, Th2 or Th17 cytokines upon recognition of cognate glycolipid ligands. A variety of microbially derived ligands have been described including glycolipids from Strep. Pneumonia or Borrelia Burgdorferi. iNKT cells also directly respond to certain cytokines such as IL-12, thereby mimicking innate cells. We have recently described an important regulatory role for iNKT cells in dampening combined gut and joint inflammation in spondyloarthritis (SpA). In men, iNKT cells are also expanded within the inflamed joint of SpA patients where they seem to undergo some degree of functional impairment by upregulation of coinhibitory molecules. Together with other regulatory T cell subsets, iNKT cells establish a naturaly regulatory feedback loop that acts to dowmodulate inflammation and aims to resolve ongoing inflammation. <h3>Disclosure of Interest</h3> None Declared
Treatment of a completely obstructed colonic anastomotic stricture using a CT-guided endoscopic rendezvous technique
Authors: M. Albertsmeier, P. Rittler, R.-T. Hoffmann, F. Spelsberg
Journal: Endoscopy (2011)

Although anastomotic strictures are a recognized complication of colorectal surgery, complete colonic anastomotic obstruction from benign disease is rare and there are few reports of endoscopic management [1] [2] [3] [4]. Here, we describe a case of successful endoscopic treatment using a novel combined anterograde-retrograde endoscopic rendezvous technique facilitated by CT-guided fluoroscopy.
Abstract 3120: Evidence supporting a role of ETV4 in pancreatic cancer pathogenesis.
Authors: Sanjeev K. Srivastava, Nikhil Tyagi, Gurpreet Kaur, Sumit Arora, Arun Bhardwaj, William E. Grizzle, Ajay P. Singh, Seema Singh
Journal: Cancer Research (2013)

Abstract The clinical outcome from pancreatic cancer has not improved over the years. This is, in part, due to not well understood disease mechanisms and consequently there is little progress made in developing intervention therapy. Many genetic alterations have been characterized in pancreatic cancer, of which some have been shown to be functionally relevant. ETV4, proto-oncogenic transcription factor, has been shown to be aberrantly expressed in various cancers types and plays important role in disease pathogenesis. The role of ETV4, however, has not been defined in pancreatic cancer so far. In this study, we investigated the pathological involvement of ETV4 in pancreatic cancer. For this, we silenced its expression in two highly tumorigenic and metastatic ETV4 overexpressing pancreatic cancer cell lines (Colo357 and ASPC1) by stable transfection of ETV4-targeted short-hairpin RNA (shRNA)-expression plasmid. Silencing of ETV4 led to a decrease in growth and clonogenicity of ETV4-silenced pancreatic cancer cells (Colo357-shETV4 and ASPC1-shETV4) as compared to their respective controls (Colo357-Scr and ASPC1-Scr). Furthermore, inhibition of ETV4 also caused suppression of pancreatic cancer cell motility and invasiveness. In contrast, ETV4-silenced pancreatic cancer cells exhibited greater cell-cell interaction as compared to their respective control cells. Immunoblot analyses demonstrated a loss of mesenchymal markers, N-cadherin, Vimentin, and twist, and gain of epithelial marker, E-cadherin, in ETV4-silenced Colo357 and ASPC1 cells, indicating a role of ETV4 in epithelial to mesenchymal transition (EMT) of PC cells. Lastly, expression analysis of ETV4 in clinical specimens demonstrated its aberrant expression in malignant pancreatic tissue, while no expression was detected in the normal pancreas. Altogether, these findings provide first experimental evidence for a functional role of ETV4 in pancreatic cancer. Citation Format: Sanjeev K. Srivastava, Nikhil Tyagi, Gurpreet Kaur, Sumit Arora, Arun Bhardwaj, William E. Grizzle, Ajay P. Singh, Seema Singh. Evidence supporting a role of ETV4 in pancreatic cancer pathogenesis. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3120. doi:10.1158/1538-7445.AM2013-3120
PREVALENCE AND PATTERN OF DYSLIPIDEMIA IN 2500 ADOLESCENTS IN SUBURBAN INDIA
Authors: Sonam Puri, Sonika Puri, Harmeet Singh Rehan, Anil Sabharwal, Rashmi Nanda, Satish Kumar Aggarwal, Madhur Yadav, Raman Puri
Journal: Journal of the American College of Cardiology (2015)