tbuckley/cabot-search huggingface dataset:
Abstract PD01-05: A Combination of HDAC Inhibitor Entinostat (MS-275), All Trans Retinoic Acid (ATRA) and Chemotherapy Drug(s) Causes Regression of Established Xenografts of Triple Negative Breast Cancer
Authors: NK Nguyen, P Korangath, G Sabnis, A Brodie, P Ordentlich, V Stearns, S. Sukumar
Journal: Cancer Research (2010)

Abstract Triple negative breast cancer (TNBC) is a subgroup of breast cancer that rapidly develops resistance to chemotherapy. Currently, TNBC patients are treated with anthracycline and taxane-based regimens. These treatments result in some improvements in the clinical outcomes; however, they are associated with poor prognosis. Also, endocrine therapies are ineffective since the tumors do not express the estrogen receptor. Recent studies have shown that histone deacetylase (HDAC) inhibitors could reverse the epigenetic profile of some genes, including ER, EGFR, and RAR≥2. We have recently shown that treatments that combine the HDAC inhibitor, Entinostat (MS-275), with the aromatase inhibitor, Letrozole, result in regression of xenografts of MDA-MB-231 breast cancer cells. Since RAR≥2 is re-expressed as well, we reasoned that combining epigenetic therapy using MS-275, with differentiation therapy using a RAR≥2 agonist (ATRA) will provide an effective combination of drugs against TNBC. We also examined possible potentiation of anticancer activity of these agents by low nontoxic doses of chemotherapy (doxorubicin and paclitaxel). In vitro, human TNBC cell lines, MDA-MB-231, HCC 1143, SUM159, SUM149 and BT20 responded significantly better to the triple combination compared to the drugs used singly or in combination with MS-275. Upon treatment, re-expression of the silenced RAR≥2 and downstream effectors was observed in the cell lines in vitro and in tumor xenografts of MDA-MB-231 cells. Subcutaneous tumor growth in immunodeficient mice was strongly inhibited (10/14 significant regression) using a combination of MS-275, ATRA and low dose Doxorubicin, compared to the drugs administered singly or in combination with MS-275. No significant weight loss was observed in any of the groups. This combination was then used to treat tumor xenografts of SUM159, another TNBC cell line, and gave a promising result. Furthermore, combination of MS-275, ATRA, and a clinical dose of Paclitaxel can effectively inhibit growth of MDA-MB-231 and SUM159 cells. These results suggest that combination of HDAC inhibitor and RAR≥2 agonist with low dose chemotherapy has the potential to be an effective treatment against TNBC. This study provides encouraging new information for a nontoxic, and novel combination of drugs which could be effective against TNBC. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr PD01-05.
Splenic Uptake of Tc-99m MDP in a Patient With Hemochromatosis
Authors: SUSHIL S. SABNIS, MICHAEL J. BLEND
Journal: Clinical Nuclear Medicine (1999)

A 43-year-old man with a history of a liver transplantation for hepatocellular carcinoma and hemochromatosis was admitted because of syncope. His serum ferritin level was elevated. A bone scan revealed posttraumatic changes in the ribs and diffuse uptake in the spleen. This splenic activity, which has been reported in other iron deposition diseases, was thought to be related to the patient's hemochromatosis. Obviously, previous radionuclide imaging must be excluded when splenic uptake is seen on a bone scan.
FRI0029 Nucb2/nesfatin-1: a new adipokine expressed in human and murine chondrocytes with pro-inflammatory properties
Authors: M. Scotece, J. Conde, V. Lopez, J. Pino, J. J. Gomez-Reino, O. Gualillo
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Nesfatin-1 is a recently identified satiety-inducing adipokine determined in hypothalamic regions that regulates energy balance [1]. It is an integral regulator of energy homeostasis and a putative glucose-dependent insulin coadjuvant [2]. Nesfatin-1 has strong similarities in terms of metabolic actions with other members of adipokine superfamily, such as leptin, adiponectin and lipocalin-2. These factors have been recently identified as active players in the regulation of physiological and pathological processes, such as inflammation and immune response in whole joint tissues and in rheumatic diseases, such as osteoarthritis (OA) and/or rheumatoid arthritis (RA). <h3>Objectives</h3> So far, no data exist on nesfatin expression, regulation, and secretion in human and murine chondrocytes. We therefore investigated NUCB2/nesfatin-1 gene and protein expression in human and murine chondrocytes. For completeness, the effect of NUCB-2 on pro-inflammatory cytokines expression was also studied <h3>Methods</h3> NUCB-2 mRNA expression was studied by qRT-PCR. Protein secretion was measured by BioPlex assay and Western blot analysis. Peptide localization was performed by laser confocal microscopy. <h3>Results</h3> First, we demonstrated cytoplasmic localization of NUCB-2 peptide in both human and murine chondrocytes. We present evidence that both mRNA and protein expression of NUCB-2 were increased during the differentiation of ATDC5 murine chondrocyte cell line Furthermore, we demonstrated that nesfatin-1 induces IL-6 and MIP-1α mRNA expression and protein secretion in ATDC5 cells challenged with IL-1. Finally, nesfatin-1 provoked a clear and a direct induction of pro-inflammatory agents, such as COX-2 and IL-8 in human chondrocytes. <h3>Conclusions</h3> NUCB-2/Nesfatin-1 is synthesized and produced by human and murine chondrocytes, in which it exerts a clear pro-inflammatory activity. <h3>References</h3> Oh-I S, Shimizu H, Satoh T, Okada S, 2006 Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 443:709-71 Ramanjaneya M, Chen J, Brown JE, Tripathi G, 2010 Identification of nesfatin-1 in human and murine adipose tissue: a novel depot-specific adipokine with increased levels in obesity. Endocrinology 151(7):3169-80 <h3>Disclosure of Interest</h3> None Declared
Hazardous Waste Site Remediation, Neighborhood Change, and Neighborhood Quality.
Authors: M Greenberg, D Schneider
Journal: Environmental Health Perspectives (1994)

We tested the hypothesis that neighborhoods with hazardous waste sites may no longer be undesirable places to live if they have been at least partly remediated. We collected 377 questionnaires (42% response rate) administered from within one-half mile of the number 1, 4, and 12 hazardous waste sites on the National Priority List (Superfund). These neighborhoods were rated higher quality than neighborhoods with unremediated hazardous waste sites and about the same as neighborhoods in northern New Jersey and the United States as a whole. Newer residents considered these formerly tainted areas to be opportunities to upgrade their housing and living conditions. Long-term residents retained the negative image of the blemished neighborhood.
Abstract 3469: Effect of age and mutagen on the enriched murine mammary epithelial stem and progenitor cell population
Authors: Abhik M. Bandyopadhyay, Keya De, Tanuka Biswas, Maryanne Herzig, Christi A. Walter, LuZhe Sun
Journal: Cancer Research (2010)

Abstract Epidemiological studies indicated that aging is a significant risk factor for breast cancer. Yet, there is insufficient knowledge on the mechanisms affecting breast cancer initiation and promotion in older women. Recent studies indicated epithelial stem/progenitor cell population, responsible for lifelong tissue maintenance and regeneration, as a target of transformation. It has been reported earlier by serial transplantation of murine mammary tissues that functional mammary outgrowths indicating stem cell function is unaffected by the age of the donor. But it is unclear that whether spontaneous or induced mutagenesis in the aging stem/progenitor cell population has the potential to impair stem/progenitor functions and/or to initiate tumorigenesis. In this study, first we have isolated lineage negative (CD31−CD45−Ter119−) mammary epithelial cells (Lin−MEC) from the thoracic/inguinal mammary glands of young (6 months, n=7) and old (28 months, n=6) mice by serial enzymatic dissociation followed by magnetic nanoparticle separation method (StemCell Technology). These cells were utilized to form mammospheres, representing mammary stem/early progenitor cells. Although the frequency of mammosphere formation remained the same, the number of larger mammospheres (&amp;gt;60µm) from old mice were significantly lower than the young mice, probably indicating decreased stem cell activity in the older population. To study the effect of mutagen in vivo, we treated two young and two old mice with methyl nitrosourea (MNU, 25mg/kg body weight) once a week for three consecutive weeks. Animals were sacrificed one week after the last treatment. Both total and larger mammospheres (&amp;gt;60µm) from the isolated Lin−MEC of the treated older mice were observed in significantly higher number than the treated younger mice. In a recent clinical study larger mammospheres (&amp;gt;50µm) obtained from metastatic breast cancer cells has been correlated to their ability to induce tumors. These Lin−MEC cells were also analyzed and compared for DNA damage by alkaline comet assay and a higher percentage of cells (&amp;gt;10%) with irreversible DNA damage in the older population were observed compared to younger population (&amp;lt;2%) indicating probable lower DNA repair capacity of the former cells. Thus, our preliminary study indicated that enriched stem/progenitor cells in the old mice with decreased in vitro stem cell activity might have the potential to be vulnerable to DNA damage by mutagens and subsequent tumorigenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3469.
MOLECULAR GENETIC DETERMINANTS OF LONG-TERM SURVIVAL WITH GLIOBLASTOMA
Authors: M. Weller, R. G. Weber, V. Riehmer, K. Kaulich, E. Willscher, H. Wirth, J. Gietzelt, B. Hentschel, M. Westphal, M. Simon, G. Schackert, J. Schramm, J. Matschke, M. C. Sabel, D. Gramatzki, J. Felsberg, C. Hartmann, J. P. Steinbach, U. Schlegel, W. Wick, B. Radlwimmer, T. Pietsch, J. C. Tonn, A. von Deimling, H. Binder, M. Loeffler, G. Reifenberger
Journal: Neuro-Oncology (2014)

BACKGROUND: The prognosis of glioblastoma, the most malignant type of glioma, is still poor, with only a minority of patients showing long-term survival of more than three years after diagnosis. We aimed to elucidate the poorly characterized molecular aberrations in glioblastomas of longterm survivors. METHODS: We performed genome- and/or transcriptome-wide molecular profiling of primary tumor samples from 94 glioblastoma patients of the German Glioma Network, including 28 longterm survivors with >36 months overall survival (OS), 20 short-term survivors with <12 months OS, and 46 patients with intermediate OS. Integrative bioinformatic analyses were performed to characterize molecular aberrations in the distinct survival groups considering established molecular markers such as isocitrate dehydrogenase 1 or 2 (IDH1/2) mutations, and O6-methylguanine DNA methyltransferase (MGMT) promoter methylation. RESULTS: Patients with long-term survival were younger and more often had IDH1/2-mutant and MGMT promoter-methylated tumors. Gene expression profiling revealed over-representation of a distinct (proneural-like) expression signature in long-term survivors that was linked to IDH1/2 mutation. However, among the IDH1/2-wildtype patients, tumors from long-term survivors did not show distinct gene expression profiles and included proneural, classical and mesenchymal glioblastoma subtypes. Genomic imbalances also differed between IDH1/2-mutant and -wildtype tumors, but not between survival groups of IDH1/2-wildtype patients. CONCLUSIONS: Our data support an important role for MGMT promoter methylation and IDH1/2 mutation in glioblastoma long-term survival and corroborate the association of IDH1/2 mutation with distinct genomic and transcriptional profiles. However, IDH1/2-wildtype glioblastomas from long-term survivors do not carry distinct DNA copy number changes or gene expression signatures, indicating that host factors play a major role in allowing for long-term survival. SECONDARY CATEGORY: Clinical Neuro-Oncology.
MICROVASCULAR ANGINA IN THE ABSENCE OF CORONARY ARTERY DISEASE IS COMMON IN UNDIFFERENTIATED CHEST PAIN PATIENTS IN THE EMERGENCY DEPARTMENT
Authors: Basmah Safdar, Gail D’Onofrio, James Dziura, Raymond Russell, Matthew Naftilan, Caitlin Johnson, Albert Sinusas
Journal: Journal of the American College of Cardiology (2016)
A7.5 Limb Pain and swelling in children: sometimes it's not inflammation!
Authors: Susana Fernandes, José António Melo Gomes
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background and Objectives</h3> Algodystrophy or Reflex Sympathetic Dystrophy is a localised chronic pain syndrome characterised by extreme and persistent pain, functional disability and refusal of mobilisation of a specific segment. Algodystrophy in children has several specific characteristics that are distinct from the disease seen in adults. Psychological stress and other psychological comorbidities play a prominent role in children, with frequent reports of a previous history of familiar problems, bullying or sexual abuse. Significant preceding trauma is not a common event in most paediatric series, as well as previous severe disease. This condition is more common in adolescent girls and pain typically occurs in a single lower extremity. Radiographs of the affected extremity are usually normal in paediatric patients and technetium bone scintigraphy usually show decrease uptake. Pharmacologic agents have a limited role in the treatment of these children. A multidisciplinary approach should be performed, including psychological, behavioural and occupational therapy. The authors present three case reports of Algodystrophy in children. <h3>Material and Methods</h3> Three patients from a Portuguese Pediatric Rheumatology centre. <h3>Results</h3> The first case is a ten year old girl presenting with severe arthralgia, diffuse edema and refusal to use the left foot which was experience a history of bulling in school. The second report of an eight year old girl, with a previous injury reported, presenting with arthralgia, allodynia and refusal to use the right foot, also with a previous history of bullying. And a third case of sixteen year old girl with severe pain, edema, cyanosis and functional impairment of her right hand that progress to addition of her left leg in which a history of previous sexual abuse has been found. One of these patients has been previous and wrongly treated with anti-TNF agents, without any clinical result. <h3>Conclusions</h3> The diagnosis of Algodystrophy in children must lead to a careful investigation of an underlying psychological cause. Its correction is the only way that allows a correct approach of these children and adolescents and enables to provide the best medical care.
Abstract 2432: AMF/PGI modulates EGF-induced invasion of breast cancer cell line via positive feedback loop
Authors: Dhonghyo Kho, Avraham Raz
Journal: Cancer Research (2011)

Abstract Phosphoglucose isomerase (PGI/AMF) is well established to promote cell migration as an autocrine motility factor. It remains to be established which microenvironmental signals enhance the secretion of AMF from tumor cells. Herein, we found that epithelial growth factor (EGF) enhances the secretion of AMF in the breast cancer cell line, T47D. Immunoprecipitation study shows that Caisen kinase 2α (CK2α) increases the phosphorylation of intracellular AMF. Therefore, increasing secretion of AMF and the extracellular AMF enhances the downstream cascade of EGF such as Phospho-ERK and β-catenin stabilization. In the transwell motility study, EGF-induced invasion increases synergistically with purified AMF. To confirm this, we demonstrate that the knockdown of AMF by siRNA treatment suppresses EGF-induced invasiveness. These findings imply that AMF may modulate the EGF-induced invasion in the tumor microenvironment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2432. doi:10.1158/1538-7445.AM2011-2432
AB1164-HPR Correlation of Dynamic Postural Control and Hip Muscle Strength: Table 1.
Authors: A.P. Marques, A.P.M.C. Carvalho e Silva, G.P.L. Almeida, M.O. Magalhães
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Chondromalacia patellae is a common cause of anterior Knee pain, especially in young athletes or sedentary [1]. These individuals often have diminished muscle strength of the hip extensor, abductor, responsible for balance control during dynamic activities [2]. The pain and hip muscle weakness could alter dynamic postural control [3]. <h3>Objectives</h3> Correlated anterior-posterior and medial-lateral dynamic postural control with hip extensor and abductor muscle strength. <h3>Methods</h3> This study examined 25 women with Chondromalacia patellae (CP) n=25; age 25,26 (6,6), weight 60,9 (10,2) and height 163 (8,0). Postural control was evaluated in a force platform <i>NeuroCom Balance Master</i>. The dynamic control was evaluated by the step up and down test in 20 cm step to assessed anterior-posterior and medial lateral displacement of the pressure of center (COP). The hand-held dynamometer was used to evaluate the isometric hip muscle strength, which was normalized with body weight. Pain was assessed with a visual analog scale (VAS) and function by the scale of activities of daily living (ADLS). The Pearson correlation was used to verify the relation between COP displacement and hip muscle strength, using the followed guide: &lt;0.4 = lower; 0.4-0.7 = good; &gt;0.7 = high correlation. A significance level was α=0.05. <h3>Results</h3> There is a good positive correlation between anterior-posterior displacement of the COP and hip extensor strength in individual with chondromalacia patellae. The individuals show moderate pain 5.7 (1.6) and minimal functional disability 70.4 (7.5). <h3>Conclusions</h3> We observed a significant correlation between hip extensor and anterior-posterior showing the influence of the muscle strength in dynamic postural control. The positive relation may be by the activity chosen which need to move the center body forward. These results suggest the influence of the hip strength in dynamic postural control in individuals with chondromalacia patellae. <h3>References</h3> Casscells SW. Chondromalacia of the patella. J Pediatr Orthop 1982;2:560–4. Prins MR, van der Wurff P. Females with patellofemoral pain syndrome have weak hip muscles: a systematic review. Aust J Physiother 2009;55:9-15 Lee SP. Souza RB. Powers CM. The influence of hip abductor muscle performance on dynamic postural stability in females with patellofemoral pain. Gait Posture. 2012 Jul;36:425-9. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2898
Abstract 4753: Anti-tumor effect of dendritic cell-based immunotherapy in combination with chemotherapy in the patients with advanced colorectal cancer
Authors: Shinichiro Akiyama, Jun Tsukada, Takeshi Tomoda, Hiroyuki Abe, Masato Okamoto
Journal: Cancer Research (2010)

Abstract Background and objective: Tumor-specific cytotoxic T lymphocytes (CTLs) can be activated in vivo by dendritic cell (DC)-based vaccination. However, clinical responses to the immunotherapy with DC vaccination have only been observed in a minority of patients with solid cancer. Combination with other treatment modalities such as chemotherapy may overcome immunoresistance of cancer cells. In the current study, the clinical efficacy of the DC vaccine pulsed with the peptide derived from colorectal cancer-associated antigens in combination with chemotherapy has been evaluated in the patients with advanced, inoperable colorectal cancer. Patients and Methods: Thirty-two patients with advanced, inoperable colorectal cancer refractory to standard treatment were entered the study. DCs which were generated from CD14+ monocytes from leukapheresis by 6-day cultivation with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4, were matured by OK-432, a streptococcal agent, and were pulsed with the pancreatic cancer-associated antigens, such as WT1, MUC1. These DCs (1 × 107) were intradermally administered 5 times at 14-day intervals. Results: Of the 32 patients, complete response (CR) was observed in 0 (0%), partial response (PR) in 7 (21.9%), stable disease (SD) in 12 (37.5%), progressive disease (PD) in 13 (40.6%). Response rate was 21.9%. Tumor control rate was 59.4%. Survival rate, quality of life, performance status were markedly increased. Severe side effects of more than grade 3 which were assessed in accordance with NCI-Common Toxicity Criteria v.2.0, were not observed. Conclusions: It was strongly suggested that the DC vaccination pulsed with cancer associated-peptides in combination with chemotherapy was safety and effective in the patients with the inoperable colorectal cancer refractory to standard treatment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4753.
The weightless hat: Is self-deception optimal?
Authors: Elias L. Khalil
Journal: Behavioral and Brain Sciences (2011)

Abstract There are problems with the thesis of von Hippel &amp; Trivers (VH&amp;T): (1) It entails that self-deception arises from interpersonal deception – which may not be true; (2) it entails that self-deception is optimal – which is not necessarily so; and (3) it entails that interpersonal deception is optimum – which may not be true.