tbuckley/cabot-search huggingface dataset:
AB0897 Lipid Peroxidation and Antioxidant Protection in Children with Juvenile Scleroderma and Juvenile Rheumatoid Arthritis
Authors: I. Chyzheuskaya, L. Belyaeva, T. Yuraga, H. Khrustalyova, H. Kolupaeva
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Objectives</h3> To evaluate the status of lipid peroxidation (LPO) and antioxidant protection in children with juvenile scleroderma (JS) and juvenile idiopathic arthritis (JIA). <h3>Methods</h3> Baseline demographics, clinical characteristics and disease activity parameters have been documented. Determination of parameters of LPO and the content of fat-soluble and water-soluble ability (ACL and ACW) of substances in blood serum have been carried out. <h3>Results</h3> 34 children with juvenile scleroderma (JS) and 48 children with juvenile idiopathic arthritis (JIA) were examined. At investigation the median ages at JIA were 11.9±4.3 years and at JS were 12.3±3.6 years. The median age at JIA onset was 6.8 years (STD (standard deviation) 4.3, range 0.5-14.7). The median age at JS onset was 7.4 years (STD 3.9, range 1.4-13.8). The median disease duration was 5.1 years (STD 4.0). ANA positivity has been found in 52.4%. The most frequent included JIA categories were rheumatoid factor negative polyarticular JIA with 62.5% and oligoarticular JIA with 22.9%. 14.6% of patients had a history of systemic JIA. Significant (P&lt;0.05) increase in the level of intermediate and end products of LPO in the serum of patients in clinical groups compared with the control group were revealed in the study. Content of dienkonyugates in the blood of children with JS were 6.81±1.01, in children with JIA – 3.26±0.54, in healthy children – 1.65±0.4. Content of dienketones children with JS was 9.88±1.24, in children with JRA – 4.22±0.39, healthy children – 0.19±0.08. Content of malonic dialdehyde in children with JS was 17.9±2.7 mmol/l, in children with JIA – 9.14±1.84 mmol/l, in healthy children – 6.96±1.42 mmol/l. Significant (P&lt;0.01) reduction ACW and ACL substances in the ability of blood serum of children both clinical groups compared with the control group were established. ACW content in children with JS was 6.88±1.24 mmol/l in children with JIA – 11.52±5.6 mmol/l in healthy children – 13.72±5.24 mmol/l, the content of ACL in children with JS – 5.16±1.03 mmol/l in children with JIA – 8.11±3.95 mmol/l in healthy children – 8.81±3.5 mmol/l. <h3>Conclusions</h3> The findings suggest that activation of lipid peroxidation and violation processes of antioxidant protection in children with JIA and JS can help maintain a high intensity of immune inflammation in this pathology. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5746
Abstract 2971: The modulation checkpoint signaling by natural products during the DNA damage response
Authors: Hiroshi Nishida, Kayoko Kawakami, Naoto Tatewaki, Masao Hirayama, Nobuo Ikekawa, Tetsuya Konishi
Journal: Cancer Research (2011)

Abstract ATM (ataxia telangiectasia mutated) and ATR (AT and Rad3 related) protein kinases play a crucial role in cellular DNA damage responses. The inhibition of these kinases and/or related signaling molecules lead to an abolishment of one such signal pathways termed as checkpoints. It is expected that the discovery of checkpoint modulator such as natural products will be an effective assistance of anti-cancer therapy with lower side effect. In the present study, we demonstrated the inhibitory effect of Squalene (SQ), flavonoids derived from Persimmon leaves (PL) and Schisandrin-B (SchB) on ATM/ATR-dependent checkpoint signaling pathway. The cell viability was decreased by SQ, PL or SchB treated A549 adenocarcinoma after gamma-irradiation (IR) or UV exposure. In each SQ, PL and SchB treated cells, G2/M checkpoint activity was abolished in DNA damaged cells. In vitro kinase assay revealed that PL and SchB inhibits ATM and ATR, respectively. SQ, on the other hand, showed no effect on both kinases. Interestingly, SQ increased Wip1 protein expression in unperturbed cells, and suppressed ATM activation in IR treated cells. Consistent with potential inhibition of ATM by SQ or PL, IR-induced phosphorylations of ATM effectors such as p53 (S15) and Chk1 (S317) were remarkably inhibited by the treatment of SQ or PL. SchB inhibited the specific phosphorylation of these effectors by ATR. Moreover, Xenograft and allograft studies with natural products were demonstrated that the tumor growth of both cancer cells in vivo were obviously inhibited by SQ, PL or SchB treatment. These results suggest that some natural products such as SQ, PL and SchB might be applicable to the sensitization of anti-cancer therapies such as radio- /chemotherapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2971. doi:10.1158/1538-7445.AM2011-2971
1270 ALTERED FATTY ACID PROFILE IN LIVERS OVEREXPRESSING THE Igf2 mRNA BINDING PROTEIN p62: INDUCTION OF FATTY ACID ELONGASE ELOVL6 VIA Igf2-DEPENDENT SREBP1 ACTIVATION
Authors: S. Laggai, S.M. Kessler, K. Gemperlein, J. Haybaeck, R. Mueller, A.K. Kiemer
Journal: Journal of Hepatology (2013)
1333 PRELIMINARY EVIDENCE OF A REDUCED SERUM LEVEL OF FIBROBLAST GROWTH FACTOR 19 IN PATIENTS WITH BIOPSY-PROVEN NONALCOHOLIC FATTY LIVER DISEASE
Authors: F. Eren, R. Kurt, F. Ermis, O. Atug, N. Imeryuz, Y. Yilmaz
Journal: Journal of Hepatology (2013)
AEROBIC EXERCISE TRAINING AND HEALTH STATUS IN AMBULATORY HEART FAILURE PATIENTS WITH A REDUCED EJECTION FRACTION: AN ANALYSIS FROM THE HF-ACTION TRIAL
Authors: Andrew P. Ambrosy, Lukasz Cerbin, Adam DeVore, Stephen Greene, William E. Kraus, Christopher O’Connor, Ileana Pina, David Whellan, Daniel Wojdyla, Angie Wu, Robert Mentz
Journal: Journal of the American College of Cardiology (2016)
Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR‐1+CD8+ T Cells
Authors: Kathrin Rothe, Dagmar Quandt, Kristin Schubert, Manuela Rossol, Maria Klingner, Simon Jasinski‐Bergner, Roger Scholz, Barbara Seliger, Matthias Pierer, Christoph Baerwald, Ulf Wagner
Journal: Arthritis & Rheumatology (2015)

Objective Leukocyte immunoglobulin‐like receptor 1 (LIR‐1) is up‐regulated by cytomegalovirus (CMV), which in turn, has been associated with premature aging and more severe joint disease in patients with rheumatoid arthritis (RA). The aim of this study was to investigate the expression and functional significance of LIR‐1 in CMV‐positive RA patients. Methods We determined the phenotype, cytolytic potential, CMV‐specific proliferation, and HLA–G–triggered, LIR‐1–mediated inhibition of interferon‐γ secretion of LIR‐1+ T cells in RA patients and healthy controls. Results We found increased frequencies of CD8+ T cells with CMV pp65–specific T cell receptors in CMV‐positive RA patients as compared to CMV‐positive healthy controls. CMV‐specific CD8+ T cells in these patients were preferentially LIR‐1+ and exhibited a terminally differentiated polyfunctional phenotype. The numbers of LIR‐1+CD8+ T cells increased with age and disease activity, and showed high levels of reactivity to CMV antigens. Ligation of LIR‐1 with soluble HLA–G molecules in vitro confirmed an inhibitory role of the molecule when expressed on CD8+ T cells in RA patients. Conclusion We propose that latent CMV infection in the context of a chronic autoimmune response induces the recently described “chronic infection phenotype” in CD8+ T cells, which retains anti‐infectious effector features while exhibiting autoreactive cytolytic potential. This response is likely dampened by LIR‐1 to avoid overwhelming immunopathologic changes in the setting of the autoimmune disease RA. The known deficiency of soluble HLA–G in RA and the observed association of LIR‐1 expression with disease activity suggest, however, that LIR‐1+ T cells are insufficiently controlled in RA and are still likely to be involved in the pathogenesis of the disease.
Vitreous Opacities Diagnostic of Familial Primary Amyloidosis
Authors: Herbert E. Kaufman, Louis B. Thomas
Journal: New England Journal of Medicine (1959)

IN 1953 Kantarjian and DeJong1 first described the A presence of vitreous opacities in a family with amyloidosis. Subsequently, Rukavina and his coworkers2 reported cases with vitreous opacities in another family, and Falls et al.3 suggested that the morphology of the opacities, especially when they occurred in the absence of signs of intraocular inflammation, might be of diagnostic aid. In 1958 the vitreous opacities occurring in a case of familial primary amyloidosis were proved to consist of amyloid.4 This indicated that the distinctive appearance of these deposits was indeed pathognomonic of the disease.Primary amyloidosis ranks with disseminated lupus erythematosus, . . .
A Solitary Large Radioiodine Accumulative Lung Lesion in High-Dose 131I Therapeutic Scan
Authors: Won Hyoung Lee, Jung Mi Park, Jeong Ja Kwak
Journal: Clinical Nuclear Medicine (2014)

We reported a large radioiodine accumulative lung lesion on I therapeutic whole-body scan performed in a 50-year-old woman for thyroid cancer ablation therapy. Previously, her chest radiography and contrast-enhanced chest CT images showed bronchial atresia in the left upper lobar bronchus and mucus-filled dilated distal bronchus. Bronchial mucocele was confirmed by CT-guided percutaneous transthoracic needle aspiration. Bronchial atresia is a rare congenital abnormality associated with the mucocele.
Abstract 4073: Comprehensive cell health analysis on a novel multi-parametric cell analyzer
Authors: Katherine Gillis, Asima Khan, Julie Clor, Kamala Tyagarajan
Journal: Cancer Research (2012)

Abstract The study of anti-cancer compounds and cancerous cell lines requires access to cell health information that includes parameters such as cellular vitality, cell cycle and apoptosis and provides a complete picture of cell health. Traditionally, expensive and complicated instrumentation has been required for comprehensive cell health analysis. Here we present data from a novel, low-cost, easy to use, compact, high performance instrument, the Muse Cell Analyzer which provides multi-dimensional cell health information. The instrument utilizes a combination of highly simplified mix and read assay protocols with a touch-screen interface which greatly simplifies data acquisition and analysis. The objective of this study was to evaluate performance of Muse Count and Viability Assays when compared to traditional predicate methods including hemacytometers and automated image based systems. The mix and read format of the Muse™ Count &amp; Viability assay provides quantitative detection of multiple suspension cell lines (e.g. Jurkats, Hl60s, K562s) and adherent cell lines (e.g. CHOs, PC3s, MCF-7s) across a wide range of concentrations and viabilities. Comparison of count and viability data from multiple platforms indicate that count and viability information on a vast variety of cancer cell lines can be robustly and accurately obtained on the Muse Cell Analyzer and the system depicts good linearity over a wide concentration range. Superior precision was obtained on the Muse Cell Analyzer for a variety of sample types with %CV ranging from 1-8% compared to %CV of 5-20% for predicate methods on the same samples. Cytotoxicity effects of anti-cancer compounds such as staurosporine, gambogic acid etc.on multiple cell types could be reliabily obtained on the instrument platform. The mix and read format of the Annexin V &amp; Dead Cell assay and simplified Cell Cycle protocol on the instrument further allows for the easy and reliable study of impacts of anti-cancer compounds. The combination of three critical assays for cell count and viability, apoptosis and cell cycle status on a simple low cost instrument makes the Muse Cell Analyzer a versatile and accurate solution for comprehensive cell health analysis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4073. doi:1538-7445.AM2012-4073
Dystonic Reactions to Prochlorperazine in Hypoparathyroidism
Authors: Marcus Schaaf, Charles A. Payne
Journal: New England Journal of Medicine (1966)

THE acute dystonic or dyskinetic reactions are the most dramatic and alarming extrapyramidal complication of phenothiazine drugs. A severe attack is seldom forgotten by patient or attending physician. The onset is usually sudden, with intense, intermittent and often asymmetrical muscle spasm producing torticollis, retrocollis, opisthotonos, facial grimacing, protrusion or retraction of the tongue, trismus or oculogyric crises. Involvement of the jaw muscles may be severe enough to cause oral trauma,1 2 3 break teeth or dislocate the mandible,4 and respiratory distress from retained secretions, edema of the tongue or laryngeal and pharyngeal muscle spasm has prompted tracheostomy3 or endotracheal intubation and the . . .
Adaption at Specific Loci. VI. Divergence vs. Parallelism of Polymorphic Allozymes in Molecular Function and Fitness-Component Effects Among Colias Species (Lepidoptera, Pieridae)
Authors: W. B. Watt, K. Donohue, P. A. Carter
Journal: Molecular Biology and Evolution (1996)

In lowland Cobs butterflies, genotypes of the enzyme phosphoglucose isomerase (PGI) show major differences in molecular function, from which genotypic differences in organismal performance and fitness components in the wild are accurately predictable.The alpine species Colias meadii seems to share electromorph alleles with lowland congeners at PGI and phosphoglucomutase (PGM).However, high-resolution electrophoresis finds differences between PGI electromorphs of meudii and those of lowland taxa.Common C. meadii genotypes differ in thermal stability and are less thermally stable than similar electromorph genotypes in lowland Colius eurytheme.These meudii genotypes show heterozygote advantage in the kinetic parameters K,,, and V,JK,,, (and differ sharply from genotypes of C. eurytheme).The thermally more stable homozygote is the kinetically less effective one, extending the tradeoff of kinetics vs. stability in PGI homozygotes, seen in lowland taxa, to C. meudii.Positive evidence is given for the absence of assortative mating and segregation distortion at both PGI and PGM.The functional differences among PGI genotypes explain previously observed heterozygote advantage in flight capacity and survivorship and correctly predict heterozygote advantage in male mating success, of C. meudii' s PGI genotypes.Though functional information is not yet available on C. meudii PGM variants, these also show heterozygote advantage in male mating success but do not interact with PGI.Thus, differences in molecular function result in fitness component differences among PGI allozymes in alpine Cobs, as well as in lowland ones.This is the more remarkable because the parallelism is not based on allelic identity.These results support expectations of evolutionary bioenergetics and emphasize the hazards of using ordinary electrophoresis to infer allozymes' identity among taxa.In alternative scenarios for Colius' PGI evolutiondivergence from ancestral polymorphism, or independent origin-these results suggest major constraint, based in protein structure, on ability of PGI alleles to maximize fitness-related biochemical performance when homozygous.
PTU-028 Symptom Assessment of Patients in the Cheshire Bowel Cancer Screening Programme with a finding of Cancer: Abstract PTU-028 Table 1
Authors: A Bond, J Everall, I London, K Koss
Journal: Gut (2013)

<h3>Introduction</h3> When attending the bowel cancer screening (BSC) programme patients undergo pre-colonoscopy assessment of their symptoms. This is conducted by the specialist screening practitioner for the BCS programme. Following a diagnosis of bowel cancer at colonoscopy the questions were asked again, after a 3–6 month period. Comparison could then be made to assess the validity of the pre-assessment questionnaire. It would also allow us to look at whether patients reported all symptoms during pre-assessment. <h3>Methods</h3> All patients with a diagnosis of bowel cancer, at the Cheshire Bowel Cancer Screening Programme, were asked to complete a second symptom assessment questionnaire. A direct comparison between the reported symptoms pre and post colonoscopy could then be made. The post colonoscopy questioning also included duration of symptoms. <h3>Results</h3> In total 83 patients replied to the second questionnaire. The symptoms reported pre and post colonoscopy were similar. PR bleeding was the most commonly reported symptom in the pre and post assessment questionnaire. 42 (49%) patients reported this in the pre questioning, whilst 31 reported in after their diagnostic procedure. The next most common symptom was change in bowel habit. The number of patients reporting this was the same in both the pre and post assessment (n = 25). Further symptoms were assessed in the post procedure questionnaire, 19% and 14% of patients report straining and abdominal bloating respectively. There was also an increase in the reporting of family history of malignancy in the post assessment process, namely of Breast and Ovarian carcinoma. 30% of patients with diagnosed bowel cancer reported a family history of bowel cancer during the pre and post assessment questionnaire. Only 11% patients reported both a family history of bowel cancer and PR bleeding during pre-assessment. When comparing multiple symptoms the results in the two assessments were fairly similar. 16 patients in the pre-assessment reported both bleeding and change in bowel habit, with 14 in the post assessment group. Table showing numbers of patients reporting combinations of symptoms, pre and post assessments. <h3>Conclusion</h3> PR bleeding is the most reported symptom in those found to have bowel cancer. However, more than 50% patients with diagnosed bowel cancer did not report PR bleeding. When combining all symptoms together we found that 23% patients were totally asymptomatic. Comparison of questionnaires collected prior and post colonoscopy on bowel cancer screening programme has shown accurate and consistent reporting of symptoms. <h3>Disclosure of Interest</h3> None Declared
OP0211 APOE genotypes are associated with reduced joint damage and increased lipid levels in patients with rheumatoid arthritis:
Authors: M. Mæhlen, S.A. Provan, A.G. Semb, T. Uhlig, D. van der Heijde, I.C. Olsen, T.K. Kvien, B.A. Lie
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Rheumatoid arthritis (RA) is a disease characterised by joint inflammation, in addition RA patients often suffer from dyslipidemia and cardiovascular disease (CVD). Apolipoprotein E (<i>APOE</i>) genotypes are associated with lipid levels, cardiovascular disease and CRP levels in the general population. It is unknown whether <i>APOE</i> genotypes are associated with RA susceptibility or severity. <h3>Objectives</h3> We examined the associations between <i>APOE</i> genotypes and disease susceptibility and markers of disease severity in RA, including radiographic joint damage, inflammatory markers, lipid levels and cardiovascular markers. <h3>Methods</h3> 945 RA patients and 1121 controls were genotyped for two <i>APOE</i> polymorphisms. Genotype frequencies were compared between patients and controls. 205 patients (baseline disease duration &lt;4 years) had 10 year longitudinal data, with repeated assessments of CRP, erythrocyte sedimentation rate (ESR) and hand radiographs (van der Heijde Sharp scored (SHS)). We examined longitudinal associations between <i>APOE</i> genotypes and SHS, CRP and ESR, respectively. Lipid levels, carotid intima media thickness, carotid plaques, history of CVD were compared across genotypes in a cross sectional study of 136 patients. Analyses were adjusted for confounders, covariates and multiple testing. <h3>Results</h3> We found a longitudinal linear association between <i>APOE</i> genotypes and radiographic joint damage. ɛ2 carriers had highest radiographic score, followed by ɛ3/ɛ3, and ɛ4 carriers had lowest score (Coefficient (95% CI): -0.43 (-0.74 - -0.12)p=0.007). The association remained significant after adjustment for age, gender, ACPA status, DMARD treatment and time. We also found an association with total cholesterol (TC) and low-density lipoproteins (LDL) (ɛ2 &lt; ɛ3/ɛ3 &lt; ɛ4, p=0.03 and p=0.02, TC and LDL respectively, Table). Associations remained significant in multivariate analyses, where adjustments were made for age, gender and statins. No association was found with RA susceptibility, CRP, ESR, or CVD markers. <h3>Conclusions</h3> Joint damage can be viewed as cumulative inflammation over time, and our findings suggest that <i>APOE</i> genotypes are associated with joint damage in RA over a 10-year period. These findings are in accordance with a study by Toms et al which found an association between <i>APOE</i> genotypes and disease activity in RA (Journal of Rheumatology, 2011). In addition, our results indicate that <i>APOE</i> genotypes influence TC and LDL levels in RA patients which may promote atherosclerosis. <h3>Disclosure of Interest</h3> None Declared
617 FREE SERUM CORTISOL LEVELS AS A PREDICTIVE FACTOR FOR SURVIVAL IN PATIENTS WITH LIVER CIRRHOSIS AND ACUTE VARICEAL BLEEDING
Authors: C. Triantos, M. Kalafateli, D. Samonakis, K. Zisimopoulos, N. Papiamonis, A. Sapountzis, M. Michalaki, G. Theocharis, K. Thomopoulos, C. Labropoulou-Karatza, V. Kiriazopoulou, E. Jelastopulu, E.A. Kouroumalis, V. Nikolopoulou, A.K. Burroughs
Journal: Journal of Hepatology (2013)