tbuckley/cabot-search huggingface dataset:
Abstract 1694: Selective formation of the γ isomer of 1,<i>N</i>2-propanodeoxyguanosine adducts of acrolein through interactions with amine-containing molecules
Authors: Fung-Lung Chung, Mona Y. Wu, Marcin D. Dyba, Raghu G. Nath
Journal: Cancer Research (2010)

Abstract Acrolein (Acr) is a highly reactive α,β-unsaturated aldehyde found ubiquitously in the environment and also formed endogenously as a major product from oxidation of polyunsaturated fatty acids. Acr can bind to deoxyguanosine (dG) to form two regioisomers of 1,N2-propanodeoxyguanosine adducts (α- or γ-Acr-dG depending on the position of the OH group). However, in most cases γ-Acr-dG is the major adduct detected in rodent and human tissues and Acr-treated cells. Our LC-MS/MS analyses of seven human lung and liver tissues confirmed previous findings that γ-Acr-dG is the major isomer. The reasons for the apparent prevalence of γ-Acr-dG in vivo are unclear. To determine whether the reaction of Acr with cellular molecules alter isomer formation, the soluble fraction of BEAS-2B cell lysate was reacted with equimolar concentrations of Acr and dG in 100 mM sodium phosphate buffer (pH 7.4) at 37° C for 24 h. Acr-dG was detected and quantified via HPLC-UV. In reactions without cell lysate, α- and γ-Acr-dG were detected in approximately equivalent amounts; however, in the presence of cell lysate the amount of α-Acr-dG decreased compared to γ-Acr-dG in a concentration-dependent manner. Similar results were obtained in the reactions with amino acids, histones, and polyamines under the same conditions or with a Tris buffer instead of a phosphate buffer. Additionally, LC-MS/MS analysis of reactions of these compounds with calf thymus DNA rather than dG yielded similar results. These results suggest that amino compounds are needed for the selective formation of the γ isomer. To investigate the intermediate(s) involved, Acr was reacted with arginine in 100 mM sodium phosphate buffer (pH 7.4). Analyses by HPLC-UV show that an initial compound forms quickly, but decomposes over time to give rise to a stable compound. These compounds were collected separately and reacted with dG. While Acr-dG was not detected in reactions with the stable compound, the initial compound predominately gave rise to γ-Acr-dG. It is likely that a similar or related unstable intermediate formed between Acr and an amine-containing molecule is responsible for the formation of DNA adducts in vivo. (Supported by NCI grant CA043159.) Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1694.
Endoscopic Findings and Endosonographic Features of Esophageal Tuberculosis: a Report of 9 Cases
Authors: YuQian Zhou, JiRong Huo, DeLiang Liu, XueHong Wang, DaLian Ou
Journal: Endoscopy (2011)

Objective: Because of the rarity, esophageal tuberculosis is hard to diagnose correctly, which often leads to unnecessary surgeries and severe complications. In this report, we summarize the characteristics of nine cases with the conventional endoscopy and endoscopic ultrasonography (EUS). Methods: A single-center based retrospective study was performed. We reviewed the clinical, endoscopic, endoscopic ultrasonographic and pathological records of the patients with esophageal tuberculosis. These patients were clinically diagnosed from 2005 to 2009.
Appendectomy and the Risk of Tubal Infertility
Authors: Beth A. Mueller, Janet R. Daling, Donald E. Moore, Noel S. Weiss, Leon R. Spadoni, Bruce V. Stadel, Michael R. Soules
Journal: New England Journal of Medicine (1986)

We studied the importance of a history of appendectomy for appendicitis in 279 women with laparoscopically or surgically diagnosed tubal infertility and a control group of 957 fertile women. After controlling for the effects of age, use of an intrauterine device for contraception, a history of pelvic inflammatory disease, and other potential confounding variables, we found that no excess risk of tubal infertility was associated with a simple appendectomy without rupture. However, when the operation was reportedly for a ruptured appendix, the relative risk of tubal infertility was 4.8 (95 percent confidence interval, 1.5 to 14.9) for women who had never been pregnant and 3.2 (95 percent confidence interval, 1.1 to 9.6) for women with one or more previous pregnancies. We conclude that the early diagnosis and treatment of suspected appendicitis in girls and women of reproductive age may reduce the incidence of tubal infertility resulting from the sequelae of a ruptured appendix.
AB0531 Prevalence and differences of the metabolic syndrome in patients with psoriatic arthritis and ankylosing spondylitis
Authors: D. Nemes, E. Amaricai, L. Catan, M. Dragoi, D. Popa, G. Puenea, D. Andrei, R. Balacescu
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Metabolic syndrome (MetS) is regarded as one of the extra articular manifestations of psoriasis and psoriatic arthritis (PsA). On the other hand, the data about the MetS prevalence in ankylosing spondylitis (AS) are limited. <h3>Objectives</h3> To evaluate the differences of MetS in patients with psoriatic arthritis and ankylosing spondylitis and to study the possible relationship of MetS with inflammation biomarkers and patients' functional status. <h3>Methods</h3> The study was conducted on 115 AS patients and 103 PsA patients. The patients were investigated for MetS according to the National Cholesterol Education Program. Data regarding MetS (triglycerides, HDL cholesterol, fasting glucose, blood pressure and waist circumference) and inflammatory status (ESR, CRP) were recorded. The general health was evaluated by the Health Assessment Questionnaire (HAQ). <h3>Results</h3> The prevalence of MetS was significantly higher in PsA (34%) than in AS (14%) (p&lt;0.001). <h3>Conclusions</h3> No significant differences of MetS parameters were noticed between PsA and AS patients. Instead, there were recorded statistically significant differences of inflammatory biomarkers and health status. The patients with AS and MetS had a higher inflammatory activity and a decreased quality of life in comparison with the patients with PsA and MetS. <h3>References</h3> Mok CC, Ko GT, Ho LY, Yu KL. Prevalence of atherosclerotic risk factors and the metabolic syndrome in patients with chronic inflammatory arthritis. Arthritis Care Res 2011;63(2):195-202 Tobin AM, Veale DJ, Fitzgerald O. Cardiovascular disease and risk factors in patients with psoriasis and psoriatic arthritis. J Rheumatol 2010;37(7):1386-94 <h3>Disclosure of Interest</h3> None Declared
P5-17-02: Associations between Breast Cancer Patients' Satisfaction with Nursing Staff and Hospital Characteristics, Results of a German Multicenter Study.
Authors: R Wuerstlein, C Kowalski, S Diener, S Krebs, H Pfaff, N Harbeck
Journal: Cancer Research (2011)

Abstract Background Only few studies have investigated the association between breast center characteristics such as teaching status, number of patients, whether clinical trials are conducted, or whether breast care nurses are employed and patients’ perceptions of care. Objective: The aim of the study was to determine whether or not the satisfaction of newly-diagnosed breast cancer patients with nursing staff correlates with hospital characteristics after adjusting for relevant patient characteristics. The study is part of a national process-identifying project focusing on breast cancer of the MAGS NRW (Ministry of work, health and social affairs, North Rhine Westfalia, Germany). Methods: Multilevel regression analysis was applied combining data from newly-diagnosed breast cancer patients (n= 3733) on patient characteristics and satisfaction with nursing staff and data on characteristics of the hospitals (n= 93 breast centers in Germany) in which patients were treated. Patients’ perspectives were measured for six month using the Cologne Patient Questionnaire for Breast Cancer (CPQ-BC), the other informations and hospital characteristics were collected by medical personnel. Results: Full data of 2945 patients from 81 hospitals were analysed in the multilevel logistic regression model. The odds for patients being satisfied with nursing staff were significantly higher in hospitals which employed breast care nurses at the time of the survey. At the patient level, patients were significantly more likely to be satisfied with nursing staff if they were native speakers, and with higher self-rated health. Cross-level interaction analysis suggested that higher patient satisfaction resulting from employing breast care nurses was largely limited to native speaking patients. Other hospital characteristics were not significantly associated with the perception of nursing staff (teaching hospital, required number of surgeries, clinical trial participation). Conclusions: The results demonstrate that patient satisfaction with nursing staff is higher if breast care nurses are employed in the treating hospital. Further work will focus on the description of the breast care nurses work as it is interpreted individually in every breast center. However, only the satisfaction of native speakers was significantly higher when breast care nurses were employed. Implications for Practice: Findings suggest that hospitals should invest in employing specialist nurses. Special attention should be paid to the care of non-native speaking patients. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P5-17-02.
Abstract 3328: Cytotherapy with naïve rat umbilical cord matrix stem cells significantly attenuates growth of murine pancreatic cancer cells and increases mouse survival in syngeneic mice
Authors: Chiyo Doi, Dharmendra K. Maurya, Marla Pyle, Deryl Troyer, Masaaki Tamura
Journal: Cancer Research (2010)

Abstract Pancreatic cancer is one of the most aggressive human malignancies, with a very poor prognosis. It is the fourth leading cause of cancer-related morbidity and mortality in the United States. MSC derived from the human umbilical cord matrix (UCMSC) have a great potential for therapeutic use in cancer. UCMSC-based targeted gene/therapeutic-delivery and successful tumor attenuation has been demonstrated. However, the targeted stem cell therapy would be significantly safer and more applicable to human patients if unmodified postnatal stem cells alone were shown to cause tumor cell death and regress tumors, without any foreign gene transfection. Accordingly, the aim of this study was to determine the anti-cancer effect of un-engineered naïve rat UCMSC on the growth of murine pancreatic ductal carcinoma in vitro and in vivo. A mouse peritoneal model was used to test the ability of un-engineered rat UCMSC to control growth of pancreatic cancer. In addition, MTT, direct cell count, [3H] thymidine uptake, and soft agar colony assays were carried out as in vitro studies. Co-culture of rat UCMSC with PAN02 murine pancreatic carcinoma cells (UCMSC:PAN02, 1:6 and 1:3) caused G0/G1 arrest and significantly attenuated the proliferation of PAN02 carcinoma cells as monitored by MTT assay, direct cell counts, and [3H] thymidine uptake assay. Rat UCMSCs also significantly reduced PAN02 colony size and number as measured by soft agar colony assay. The in vivo mouse studies showed that rat UCMSC treatment significantly decreased the peritoneal PAN02 tumor burden 3 weeks after tumor transplantation and increased mouse survival time. Histological study revealed that intraperitoneally administered rat UCMSC survived for at least 3 weeks and the majority were found near or inside the tumor. These results indicate that naïve rat UCMSC alone remarkably attenuate the growth of pancreatic carcinoma cells in vitro and in a mouse peritoneal model. Thus, these studies imply that UCMSC could be a potential tool for targeted cytotherapy for pancreatic cancer. This work was supported by Kansas State University (KSU) Terry C. Johnson Center for Basic Cancer Research, KSU College of Veterinary Medicine Dean's fund, Kansas State Legislative Appropriation, NIH P20RR017686, P20RR015563 and R21 CA135599. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3328.
TREATMENT AND DISCHARGE PATTERNS AMONG PATIENTS HOSPITALIZED WITH NON-VALVULAR ATRIAL FIBRILLATION WHO TRANSITION FROM THE INPATIENT TO OUTPATIENT SETTING
Authors: Henry Henk, Kenneth Tuell, Kwanza Price, Jack Mardekian, Kevin Odell, Chad Patel, Mario Mendoza, Shalabh Singhal, Jeffrey Trocio, Lien Vo
Journal: Journal of the American College of Cardiology (2016)
Abstract 4364: Copy number gain of <i>PTK2</i> and <i>PIK3CA</i> mutations are common genetic alterations in ovarian clear cell carcinoma.
Authors: Heejei Yoon, Yoon-La Choi, Ji-Young Song, Ingu Do, So Young Kang, Young-Hyeh Ko, Sangyong Song, Byong-Gie Kim
Journal: Cancer Research (2013)

Abstract Ovarian clear cell carcinoma (OCCC) is a subtype of epithelial ovarian cancer and accounts for 5-25% of ovarian cancers. OCCC has shown higher resistance to first-line platinum and taxane-based chemotherapy, resulting in worse prognosis. Most common mutations occur in the genes ARID1A (46%) and PIK3CA (33%). Using array comparative genomic hybridization (aCGH), we previously identified frequent chromosomal gain at 8q24, which includes the encoding sequence for focal-adhesion kinase (FAK)/Protein Tyrosine Kinase 2 (PTK2), a protein that provides signaling and scaffolding functions at sites of integrin adhesion. To validate the copy number gain of PTK2 and correlate the copy number variations with PIK3CA mutations, we analyzed the copy number gain and protein expression levels of PTK2, and examined the PIK3CA mutations in 67 OCCC samples. We found that PTK2 copy number gain and loss were at a frequency of 23% and 25%, respectively. PTK2 gain significantly correlated with the strong expression of PTK2. PIK3CA mutations were found in 20% of the samples. About 40% of the tested samples showed PTK2 gain or PIK3CA mutation. PIK3CA mutations are not strictly mutually exclusive to PTK2 gain, but are more likely to occur in samples with PTK2 copy number normal or loss. Genetic alterations in OCCC mainly activate the PTK2/PI3K/AKT signaling pathway, which makes the pathway an attractive candidate for targeted therapeutics. We, therefore, treated OVMANA, OVISE and OVTOKO OCCC cell lines with PTK2 inhibitors (PF-562271 or PF-573228), or a combination of PF-562271 with a PI3K/mTOR dual inhibitor (BEZ235). Copy number variations of PTK2 were not related to the drug sensitivity of the OCCC cell lines. Drug sensitivity of most of the cells was enhanced toward PF-562271 than PF-573228. OVMANA cells with PIK3CA mutations were more sensitive to a combination of PI3K/mTOR and PTK2 inhibitor than the cells without PIK3CA mutations. Our findings thus suggest that the PTK2/PI3K/AKT signaling pathway is frequently activated in OCCC, and a combination of inhibitor drugs would prove to be necessary to enhance the overall drug efficacy in treating OCCC. Citation Format: Heejei Yoon, Yoon-La Choi, Ji-Young Song, Ingu Do, So Young Kang, Young-Hyeh Ko, Sangyong Song, Byong-Gie Kim. Copy number gain of PTK2 and PIK3CA mutations are common genetic alterations in ovarian clear cell carcinoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4364. doi:10.1158/1538-7445.AM2013-4364
FEASIBILITY AND VALUE OF GATED BLOOD POOL SPECT (GBPS) ASSESSMENT OF RV FUNCTION IN PATIENTS UNDERGOING LVAD IMPLANTATION
Authors: Christopher B. Link, Aditi Nayak, Luigi Lagazzi, Andrew Althouse, Jeffrey Teuteberg, Marc Simon, Robert Kormos, Prem Soman
Journal: Journal of the American College of Cardiology (2016)
AB0155 Periostin Deficiency Aggravates Inflammation and Joint Destruction in an Animal Model of Rheumatoid Arthritis
Authors: Y.S. Suh, H.-O. Kim, I.-I. Lee, H.S. Lim, Y.-H. Cheon, W.-H. Yoo, W.-S. Lee, J.-M. Kim, S.-H. Kim
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Periostin is a matricellular protein that expressed in bone, periodontal ligament, heart valve, tendon and skin. Previous study indicated that periostinis linked with several inflammatory diseases such as airway hypersensitivity, periodontal inflammation, and pulmonary fibrosis. However, there is no study to investigate the role of periostin in rheumatoid arthritis (RA). <h3>Objectives</h3> This study was performed to assess the function of periostin in K/BxN serum transfer arthritic mice. <h3>Methods</h3> Synovial tissues, serum, joint fluid, and fibroblast-like synoviocyte (FLS) were obtained from patients with RA and osteoarthritis (OA). The expression of periostin in synovial tissues was assessed by immunohistochemistry. The levels of periostin were checked by Western blot and enzyme-linked immunosorbent assay (ELISA). K/BxN serum transfer arthritis was induced in periostin knockout (KO) and age-matched wild type (WT) mice. Arthritis severity was assessed by clinical and histopathologic scoring. The levels of inflammatory cytokines in ankles and serum were measured by quantitative PCR and ELISA. The osteoclastogenesis was assessed by using bone marrow monocytes (BMM) from periostin KO or WT mice. <h3>Results</h3> Periostin was highly expressed in the synovium of RA patient than OA. The periostin levels were increased in the presence of IL-1β and TNF-α in RA-FLS. The periostin KO mice showed more severe arthritis from day 4 to day 10. The peak clinical scores were: 5.0±0.6 in WT, 7.36±0.5 in periostin KO mice; p&lt;0.01). The periostin KO mice showed aggravated histopathologic findings than WT, respectively: synovial inflammation (3.5±0.6 vs 2.2±0.4; p&lt;0.05), bone erosion (3.8±0.6 vs 1.9±0.5; p&lt;0.05) and cartilage damage (3.2±0.7 vs 1.8±0.5; p&lt;0.05). The level of IL-1b was increased in the serum of periostin KO mice than WT (18 pg/ml ±7.0 vs 5 pg/ml ±1.2; p&lt;0.01). BMMs from periostin KO mice had more TRAP-positive multinucleated cells than WT. <h3>Conclusions</h3> This study suggests that regulation of periostin contributes to pathogenesis of RA and periostin has a potential protective role in RA. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4201
F-18 FDG PET/CT and MR Imaging of Rectourethral Fistula
Authors: Ba D. Nguyen, Joseph M. Collins
Journal: Clinical Nuclear Medicine (2011)

Rectourethral fistula (RUF) is an uncommon pathologic entity of congenital or acquired origin. Acquired lesions may be iatrogenic, traumatic, neoplastic, or inflammatory. The clinical diagnosis of RUF is frequently suggested by fecaluria, pneumaturia, or rectal leakage of urine during micturition. Its imaging diagnosis is based on the demonstration of the contrast-filled fistula track on fluoroscopy/CT or fluid-enhancing sequences on MR. The authors present a case of RUF suspected by PET/CT and confirmed by MR.
The Need for Better Methods of Extrapolation
Authors: Leo Friedman
Journal: Environmental Health Perspectives (1973)

In the fall of 1966 the Food and Drug Administration established an Advisory Com- mittee on Protocols for Safety Evaluation.The first problem to be reviewed was the requirement for reproduction studies in the safety evaluation of pesticides and food ad- ditives, and the adequacy of the current me- thods involved.Reproduction studies are de- signed to give information on: (1) effects on male and female fertility, (2) effects during gestation on the mother and the fetus, (3) effects appearing after parturition on the mother (for example, lactation) and the off- spring (for example, growth, development, sexual maturation), and (4) mutagenic ef- fects, particularly those which might not appear in the first generation.The report of panel dealing with this problem was written in November 1968 and published later (1).During the time the panel was considering the problem, a re- port prepared by the Genetic Study Section under the chairmanship of Dr. James F. Crow (2), became available to us (C.C. Cockerham, personal communication).The emphasis in this particular report of the Genetic Study Section was that there is reason to be concerned about chemicals as a mut- agenic risk equivalent to radiation, possibly even more serious.The concern, of course, is with the welfare of future generations as well as with the health of contemporary pop- ulations.It is useful to emphasize again that
Laparoscopy as a Cause of a False-Positive Meckel's Scan
Authors: ELAINE C. McKEVITT, JOANNE E. BAERG, HELEN R. NADEL, ERIC M. WEBBER
Journal: Clinical Nuclear Medicine (1999)

A new cause of a false-positive result of a Meckel's scan is reported. An 11-year-old girl had a 3-week history of constant right lower quadrant pain that was initially managed by laparoscopic appendectomy. A repeated laparoscopy for persistent pain was nondiagnostic. A missed Meckel's diverticulum was considered as the cause of this pain, which prompted a Meckel scan. This scan revealed a periumbilical focus of activity that was interpreted as a Meckel's diverticulum attached to the anterior abdominal wall by a band. The laparotomy showed no Meckel's diverticulum. The false-positive result of the Meckel scan may be the result of inflammation from the periumbilical laparoscopic port site.
THE ASSOCIATION OF ADIPONECTIN WITH INCREASED ALL-CAUSE AND CARDIOVASCULAR MORTALITY AND MAJOR ADVERSE CARDIOVASCULAR EVENTS: INSIGHTS FROM THE DALLAS HEART STUDY
Authors: Guy Witberg, Colby Ayers, Aslan Turer, Eli Lev, Ran Kornowski, James de Lemos, Ian Neeland
Journal: Journal of the American College of Cardiology (2016)
Fluoroquinolone Antimicrobial Agents
Authors: David C. Hooper, John S. Wolfson
Journal: New England Journal of Medicine (1991)

THE introduction of fluoroquinolone antimicrobial agents (Fig. 1) into clinical use is an important recent advance.1-3 These drugs, also called quinolones, include norfloxacin, ciprofloxacin, ofloxacin, enoxacin, and pefloxacin. Of these, norfloxacin, ciprofloxacin, and ofloxacin have been approved for use in the United States. Quinolones are orally absorbed, are potent in vitro against a broad spectrum of bacterial species, and have favorable pharmacokinetic properties. We shall evaluate here the current status of the quinolones, considering mechanisms of action and resistance, activity in vitro, pharmacokinetics, clinical efficacy, adverse effects, and clinical uses. A discussion of the structure-activity relations of the quinolones . . .