tbuckley/cabot-search huggingface dataset:
Abstract C27: Phosphorylation of the polycomb group protein polyhomeotic (Ph) induces a relaxed chromatin state through depolymerization of the Ph SAM domain
Authors: David R. Nanves, Belinda Leal, Angela Robinson, Lijun Di, Kevin Hakala, Susan Weintraub, Chongwoo A. Kim
Journal: Cancer Research (2011)

Abstract The transcriptional repressor polyhomeotic (Ph) is part of the polycomb group (PcG). PcG proteins are responsible for repressing Hox genes, a set of transcription factors that determine segmental identification early in development. PcG proteins are highly conserved throughout evolution, and recent studies have identified that the human homologue polyhomeotic-like protein 3 (PHC3) plays a role in tumor suppression in osteosarcomas. In order to understand how PHC3 functions, we utilized a cellular system with Ph. In our study we found that Ph is phosphorylated at a Ser residue several positions before the SAM domain. This region is important for controlling Ph SAM polymerization raising the possibility that phosphorylation alters the polymeric state of Ph. Phosphorylation was detected only in wild-type Ph and a mutant intended to increase polymerization, but not on polymer deficient Ph mutants. Because Ph repressive ability is dependent on SAM polymerization, we hypothesize that phosphorylation could trigger SAM depolymerization and consequently induce a relaxed chromatin state. To this point, mutating the phosphorylated Ser to Ala produced a better transcriptional repressor than wild-type. These results point to a potential way of controlling Ph SAM polymerization where phosphorylation leads to depolymerization and a replication competent chromatin state. Upon completing replication, the original repressive epigenetic state could be inherited by the daughter cell through dephosphorylation and Ph repolymerization. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the Second AACR International Conference on Frontiers in Basic Cancer Research; 2011 Sep 14-18; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2011;71(18 Suppl):Abstract nr C27.
Abstract 1094: Role of the CRLF2 ligand TSLP in normal human B-cell development
Authors: Kimberly J. Payne, Terry-Ann Milford, Olivia Francis, Ineavely Baez, Sinisa Dovat, Rui-Jun Su
Journal: Cancer Research (2011)

Abstract B cell acute lymphoblastic leukemia (B-ALL) is the most common childhood malignancy. Defects that result in overexpression of CRLF2, a cytokine receptor component, have recently been linked with poor outcome in both high-risk and non-high-risk B-ALL. CRLF2, together with the IL-7R alpha chain, forms the signaling receptor for the cytokine TSLP (Thymic Stromal-derived LymphoPoietin). TSLP-induced CRLF2 signaling results in proliferation of murine B cell progenitors, however, the role of TSLP in murine B cell development remains controversial and little is known of its role in human B lymphopoiesis. Human TSLP and CRLF2, in contrast to other cytokine/receptor pairs that act on B cell progenitors, show low homology to their murine counterparts (&amp;lt;40%), lack species cross reactivity, and activate differing intracellular pathways than in mice. Thus, human model systems are essential for understanding the role of CRLF2 function in normal and malignant B lymphopoiesis. We have developed a novel human-only in vitro culture model that allows the study of human B cell development under highly selective cytokine stimulation. This model is based on co-culturing CD34+ hematopoietic stem cells (HSCs) with primary human bone marrow stroma supplemented with selective exogenous cytokines and/or specific cytokine neutralizing antibodies. We have previously used this model to show that IL-7 increases B cell progenitors by over 60 fold and that IL-7 is essential for normal human B cell production (J. Immunol. 2009 82:4255). Here we use our human-only in vitro model to examine the role of TSLP-induced signals in normal human B cell development. Our data show that TSLP induces an expansion of human B cell progenitor populations generated from HSCs in cord blood that is similar in magnitude to that observed for IL-7. A comparison of TSLP and IL-7 effects shows that TSLP targets early B cell progenitors (CD34+) inducing expansion of this population with limited differentiation. In contrast, IL-7 induces rapid differentiation to the CD34- stage followed by proliferation. RT-PCR and ELISA analysis show that normal human bone marrow stoma express both TSLP and IL-7, thus establishing an endogenous source of these cytokines at the site of normal and malignant hematopoiesis. Our data provide evidence that TSLP and IL-7 exert differential and stage specific effects on proliferation and differentiation of B cell progenitors and suggest that deregulation of the complex interplay between these signals in normal differentiation could set the stage for malignant transformation to B-ALL in the presence of cooperative defects in JAK2 and/or Ikaros. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1094. doi:10.1158/1538-7445.AM2011-1094
Endoscopic ultrasonography-guided drainage of a rectal mucocele after total colectomy for Crohn's disease
Authors: A. Teoh, J. Lee, C. Chong, R. Tang
Journal: Endoscopy (2013)

A 40-year-old woman was admitted with acute urinary retention and failed to wean off the Foley catheter. She had a known history of Crohn's disease and 5 years previously had undergone total colectomy and end ileostomy as treatment for massive gastrointestinal bleeding due to pancolitis. Computed tomography showed a grossly distended rectal stump up to 15 × 9 cm in size ([Fig. 1]). Sigmoidoscopy showed a blind-ended stump 5 cm from the anal verge. The overall features were compatible with a large rectal stump mucocele.
PROGNOSTIC USEFULNESS OF ADREVIEW SCINTIGRAPHY IN IDENTIFYING HEART FAILURE PATIENTS AT LOWER RISK OF DEATH DURING 5-YEAR FOLLOW-UP IN THE ADMIRE-HF STUDY
Authors: Roxy Senior, Lars Friberg, Mark Travin, Franc Hudnut, Harish Chandna, Denis Agostini, Karthikeyan Ananth, Micahael Miyamoto, Mahesh Shah, Michael Koren, Juergen vom Dahl, Wayne Levy
Journal: Journal of the American College of Cardiology (2016)
SAT0455 Preliminary Validation of a New Hybrid Measure of Muscle Strength for Juvenile Dermatomyositis
Authors: D. Marafon, A. Consolaro, C. Ferrari, S. Dalprà, A. Madeo, A. Providenti, C. Malattia, N. Ruperto, A. Martini, A. Ravelli
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Juvenile dermatomyositis (JDM) is a multisystem vasculopathic disease characterized by muscle inflammation that causes symmetrical muscle weakness. Assessment of muscle strength is, therefore, a fundamental component of the clinical evaluation of children with JDM. This assessment is traditionally made using the 8-muscle Manual Muscle Testing (MMT) and the Childhood Myositis Assessment Scale (CMAS). However, the MMT does not cover all muscles or muscle groups affected in JDM, namely abdominal muscles. Although the CMAS is more comprehensive than the MMT, it is lengthy and, therefore, may not be feasible in a busy clinical setting or when a physical therapist is not available. <h3>Objectives</h3> To investigate the construct validity of a new hybrid measure of muscle strength developed by merging the MMT with 3 items of the CMAS <h3>Methods</h3> The Hybrid MMT/CMAS (hMC) is composed of all 8 items of the MMT and the following items of the CMAS: 1) head lift; 2) sits-ups; 3) floor rise. Item 3) is recoded on 0 to 9 scale. The total score of the hMC ranges from 0 (worst) to 100 (normal). The study sample included 322 children with JDM enrolled in a multinational long-term outcome survey (Ravelli et al. AC&amp;R 2010;62:63-72), who had all measurements available. Validation procedures were conducted by comparing the correlation of the hMC, MMT and CMAS with other conventional measures of JDM activity, physical function and damage. Correlations were computed by means of the Spearman's correlation coefficient and were considered good, moderate, or poor when the r<sub>s</sub> was &gt; 0.7, 0.4-0.7, or &lt; 0.4, respectively. <h3>Results</h3> The Spearman's correlations of hMC, MMT and CMAS with other measures of disease activity and damage are presented in the table. <h3>Conclusions</h3> We have developed a new hybrid measure of muscle strength in JDM, which is more comprehensive than the MMT and more feasible than the CMAS. Overall, the construct validity of the hMC was comparable to that of the MMT and superior to that of the CMAS. <h3>Disclosure of Interest</h3> None Declared
Abstract LB-37: Cancer patient navigators of Georgia (CPNG) advocate for cancer survivors
Authors: Angie Patterson
Journal: Cancer Research (2012)

Abstract The Georgia Cancer Coalition (GCC) is a not-for-profit organization that seeks to unite government agencies, academic institutions, civic groups, healthcare organizations and survivors to reduce the number of cancer deaths in Georgia and to move Georgia to the top ranks of cancer care in the nation. The Georgia Society for Clinical Oncology (GASCO) partnered with the GCC and was awarded a grant from the American Society for Clinical Oncology (ASCO) to determine the interest in formulating a statewide multi-disciplinary organization for people who help cancer patients navigate the healthcare system. A survey was conducted and results from eighty navigators representing 25 cities demonstrated an organizational need. The Cancer Patient Navigators of Georgia (CPNG) was founded as a unique statewide organization of individuals who serve people with all types of cancer, at all stages, in all types of settings, with a diversity of education and training (from lay navigators to nurse navigators and social work navigators). CPNG's mission is to connect, educate and share best practices among patient navigators in Georgia, so that together we can successfully reduce barriers and increase access to services specifically related to cancer. The steering team developed a logo, by-laws, officers, working committees, electronic newsletters and a website, with a members-only section for social networking. Membership currently tops 230. A recent social networking survey had 100 respondents. We have conducted three annual meetings with attendance of each meeting exceeding 100. The GCC received a private gift of $250,000 to expand the patient navigation efforts in Georgia. One component of the gift is sponsoring the first Integrative Oncology conference to be held in Georgia on January 20, 2012 for the members of Cancer Patient Navigators of Georgia. In conclusion, the initial survey indicating an organizational need to create a statewide multi-disciplinary organization for people who help cancer patients navigate the healthcare system was the beginning of the Cancer Patient Navigators of Georgia (CPNG). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-37. doi:1538-7445.AM2012-LB-37
The diagnostic accuracy of high-resolution endoscopy, autofluorescence imaging and narrow-band imaging for differentially diagnosing colon adenoma
Authors: R. Sato, M. Fujiya, J. Watari, N. Ueno, K. Moriichi, S. Kashima, S. Maeda, K. Ando, H. Kawabata, R. Sugiyama, Y. Nomura, T. Nata, K. Itabashi, Y. Inaba, K. Okamoto, Y. Mizukami, Y. Saitoh, Y. Kohgo
Journal: Endoscopy (2011)

See also: Commentaire de travail de R. Sato et al., pp. 862Endoscopy 2011; 43(10): 223-223DOI: 10.1055/s-0031-1291766
Benign Paroxysmal Positional Vertigo
Authors: Joseph M. Furman, Stephen P. Cass
Journal: New England Journal of Medicine (1999)

Many patients consult their doctors because of dizziness or poor balance. Dizziness is nonspecific; it may result from a disorder of almost any organ system. Thus, the differential diagnosis for such patients is broad and should include medical, neurologic, and otologic causes. Vertigo, which is the illusory sensation of motion of either oneself or one's surroundings, may be a component of a patient's dizziness.Benign paroxysmal positional vertigo is one of the most common types of vertigo.1,2 This condition presents as dizziness or vertigo of sudden onset that is provoked by certain changes in head position. The most common . . .
LA ENDOSCOPIA DIGESTIVA EN EL TRATAMIENTO MEDIANTE PERFUSIÓN CON DUODOPA EN LA ENFERMEDAD DE PARKINSON AVANZADA
Authors: F Yepez Carpio Andres, V Pons Beltran, M Ponce Romero, M Bustamante Balen, L Argüello Viudez, G Poppele Molina, MN Braithwaite Flores Ana
Journal: Endoscopy (2013)

Introducción: La infusión continua de levodopa intraduodenal (DUODOPA®) constituye una alternativa eficaz a la infusión subcutánea de apomorfina y a la cirugía en pacientes con enfermedad de Parkinson avanzada. Objetivo: Evaluar la eficacia y seguridad de la actuación endoscópica en pacientes sometidos a inserción de PEG-J (Gastroyeyunostomia endoscópica percutánea) para perfusión continua de Duodopa. Material y Método: Análisis retrospectivo de pacientes con inserción de sonda PEG-J para tratamiento con DUODOPA. Tras confirmar la indicación desde el servicio de neurología el paciente fue remitido a la Unidad de Endoscopia Digestiva (UED) para la inserción de la sonda. El procedimiento es similar a la sonda PEG convencional (tracción) y con inserción posterior de la sonda yeyunal. Tras el procedimiento el seguimiento fue realizado por enfermería especializada y ante la aparición de complicaciones fue remitido a UED para evaluación y tratamiento. Resultados: durante un periodo de 7 años se incluyeron 19 pacientes, 11 hombres, edad media 66 años (49 – 78). En 15 pacientes el modelo de sonda utilizado fue Freka PEG CH 15 (83.3%). La técnica se realizó bajo sedación profunda con anestesista en 8 (42.1%). La extensión yeyunal se insertó en el mismo procedimiento en el 100% de los pacientes. El seguimiento medio fue de 27 meses (3 – 86). Se objetivaron complicaciones en 11 casos (61%), graves en 5 (26.3%): 1 peritonitis, 1 fijación interna de la sonda embebida en la pared gástrica que requirió solución quirúrgica, 1 caso de oclusión duodenal complicada con neumonía nosocomial y úlcera duodenal de decúbito en 2 pacientes. Complicaciones leves en 6 pacientes (31,6%). Durante el seguimiento la media de endoscopia para descartar complicación fue de 3 (0 – 6). Conclusiones: la sonda PEG-J para perfusión de DUODOPA es una técnica de inserción segura pero con frecuentes complicaciones durante el seguimiento, algunas de ellas graves.
Abstract LB-408: An integrative genomic approach uncovers oncogenic roles of YY1-signaling in hepatocellular carcinoma with stem cell-like traits
Authors: Xuelian Zhao, Stephanie Roessler, Anuradha Budhu, Zhipeng Yu, Huliang Jia, Qinghai Ye, Lunxiu Qin, Zhaoyou Tang, Xin Wei Wang
Journal: Cancer Research (2012)

Abstract Hepatocellular carcinoma (HCC) is an aggressive solid tumor with vast heterogeneity in its clinical presentation and tumor biology. Genomic and transcriptomic analyses have revealed numerous HCC subtypes that are associated with patient outcome. Recently, we have identified a subset of aggressive HCC with stem cell-like traits and elevated levels of EpCAM and AFP (EpCAMhigh/AFPhigh). To identify key pathways contributing to this subset of HCCs, we used an integrated genomic strategy by comparing gene expression profiles of a well-defined HCC cohort consisting of 38 EpCAMhigh/AFPhigh and 40 EpCAM−/AFP− HCC cases using Affymetrix U133A array analyses. We identified a robust gene expression signature that can classify these two subgroups and is predictive of HCC prognosis. We validated this signature as an independent predictor of patient survival in two additional independent cohorts of HCC samples with mixed etiologies and ethnicity. Gene Set Enrichment Analysis revealed that genes containing YY1 transcriptional factor binding sites are most significantly enriched in the signature, suggesting that the YY1 signaling pathway may be functionally critical in regulating stem cell-like HCC. Genomic analysis using the Agilent 105A human arrayCGH platform revealed that the YY1 co-activator gene YY1AP1, but not YY1, is highly amplified in a majority of EpCAMhigh/AFPhigh HCCs (85%). YY1AP1 expression in HCC was significantly correlated with its genomic amplification and was also associated with patient prognosis. Using a lentiviral construct expressing YY1AP1 shRNA either constitutively or inducibly via tetracycline, we found that silencing of YY1AP1 completely blocked the growth of EpCAMhigh/AFPhigh HCC cell lines that contain YY1AP1 gene amplification. Furthermore, we found that YY1 transcriptionally activated YY1AP1-dependent EpCAM expression. In parallel, silencing of EpCAM via RNA interference also inhibited HCC cell growth. Our results suggest that YY1-YY1AP1 signaling is an oncogenic driver critical to the development of stem cell-like HCC through activation of EpCAM. Our results also demonstrate the feasibility and power of our strategy to utilize well-defined patient samples to uncover critical pathways linked to HCC subtypes. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-408. doi:1538-7445.AM2012-LB-408
Late colonic stent perforation following chemotherapy
Authors: R. Verburg, M. Meijssen
Journal: Endoscopy (2009)

A 67-year-old patient was diagnosed as having a tumor at the splenic flexure of the colon with multiple liver metastases. They were treated with four consecutive cycles of FOX chemotherapy (leucovorin, 5-fluorouracil, and oxaliplatin). A computed tomography (CT) scan of the abdomen showed regression of liver metastases; however, the patient had obstipation, and prestenotic dilation of the colon was observed. A Wallstent enteral endoprosthesis (Boston Scientific International, Natick, MA, USA) was placed, with subsequent normal passage of stool ([Fig. 1]). Following the procedure the patient continued with chemotherapy. One month later, the patient was admitted with nausea, vomiting, and diarrhea. Gastroscopy revealed that the proximal end of the Wallstent endoprosthesis had perforated the stomach. The endoscope could pass through the endoprosthesis, and in this way it reached the descending colon ([Fig. 2]).
Satellite-Based Estimates of Long-Term Exposure to Fine Particles and Association with Mortality in Elderly Hong Kong Residents
Authors: Chit Ming Wong, Hak Kan Lai, Hilda Tsang, Thuan Quoc Thach, G. Neil Thomas, Kin Bong Hubert Lam, King Pan Chan, Lin Yang, Alexis K.H. Lau, Jon G. Ayres, Siu Yin Lee, Wai Man Chan, Anthony J. Hedley, Tai Hing Lam
Journal: Environmental Health Perspectives (2015)

A limited number of studies on long-term effects of particulate matter with aerodynamic diameter < 2.5 μm (PM2.5) on health suggest it can be an important cause of morbidity and mortality. In Asia where air quality is poor and deteriorating, local data on long-term effects of PM2.5 to support policy on air quality management are scarce. We assessed long-term effects of PM2.5 on the mortality in a single Asian city. For 10-13 years, we followed up a cohort of 66,820 participants ≥ 65 years of age who were enrolled and interviewed in all 18 Elderly Health Centres of the Department of Health, Hong Kong, in 1998-2001. Their residential addresses were geocoded into x- and y-coordinates, and their proxy exposures to PM2.5 at their addresses in 1 × 1 km grids were estimated from the U.S. National Aeronautics and Space Administration (NASA) satellite data. We used Cox regression models to calculate hazard ratios (HRs) of mortality associated with PM2.5. Mortality HRs per 10-μg/m3 increase in PM2.5 were 1.14 (95% CI: 1.07, 1.22) for all natural causes, 1.22 (95% CI: 1.08, 1.39) for cardiovascular causes, 1.42 (95% CI: 1.16, 1.73) for ischemic heart disease, 1.24 (95% CI: 1.00, 1.53) for cerebrovascular disease, and 1.05 (95% CI: 0.90, 1.22) for respiratory causes. Our methods in using NASA satellite data provide a readily accessible and affordable approach to estimation of a sufficient range of individual PM2.5 exposures in a single city. This approach can expand the capacity to conduct environmental accountability studies in areas with few measurements of fine particles. Wong CM, Lai HK, Tsang H, Thach TQ, Thomas GN, Lam KB, Chan KP, Yang L, Lau AK, Ayres JG, Lee SY, Chan WM, Hedley AJ, Lam TH. 2015. Satellite-based estimates of long-term exposure to fine particles and association with mortality in elderly Hong Kong residents. Environ Health Perspect 123:1167-1172; http://dx.doi.org/10.1289/ehp.1408264.
Abstract 763A: SUMOylation of ATF3 alters MC2R, NR5A1, STAR, and TP53 gene activities.
Authors: Chiung-Min Wang, Victoria Brennan, Ninoska Gutierrez, Xirui Wang, Lizhong Wang, Wei-Hsiung Yang
Journal: Cancer Research (2013)

Abstract Cyclic AMP-dependent transcription factor-3 (ATF3), a stress sensor/mediator, plays an essential role in cells to maintain homeostasis and has diverse functions in cellular death and survival signal pathways. ATF3 binds to the consensus ATF/cAMP response element (CRE) to regulate its downstream target genes, such as CCNE2 (Cyclin E2), CD82 (KAI1), DDIT3 (GADD153), LDLR (LDL receptor), SNAI1, TP53, and TWIST1. A previous report has shown that the activities of ATF3 are modulated by ubiquitination; however, whether it is modified by small ubiquitin-related modifier (SUMO), an important modification in regulating a wide range of cellular processes, remains unknown. The aim of this study was to investigate whether ATF3 is post-translationally modified by SUMO proteins and also to determine the role of SUMOylation of ATF3 on MC2R, NR5A1, STAR, and TP53 gene activities. Here we demonstrate that ATF3 is clearly defined as a SUMO target protein both in vitro SUMOylation assay using recombinant proteins and at the cellular levels. Furthermore, ATF3 interacted with UBE2I (Ubc9), the only SUMO E2 enzyme found so far. In addition, PIAS3β (a SUMO E3 ligase) enhanced and SENP2 and SENP7 (two SUMOylation proteases) decreased SUMOylation of ATF3, respectively. In addition, we found that ATF3 is selectively SUMOylated at lysine residue 42 but the SUMOylation/deSUMOylation does not alter subcellular localization of ATF3. We next characterized the functional role of ATF3 SUMOylation on MC2R, NR5A1, STAR, and TP53 gene expression. We found that SUMOylation of ATF3 is required for full repression of NR5A1 and TP53 genes. While SUMOylation of ATF3 is required for full activation of MC2R gene activity, SUMOylation of ATF3 further enhances repression of STAR gene activity. Overall, we provide the first evidence that ATF3 is post-translationally modified by SUMO to regulate MC2R, NR5A1, STAR, and TP53 gene activities. Citation Format: Chiung-Min Wang, Victoria Brennan, Ninoska Gutierrez, Xirui Wang, Lizhong Wang, Wei-Hsiung Yang. SUMOylation of ATF3 alters MC2R, NR5A1, STAR, and TP53 gene activities. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 763A. doi:10.1158/1538-7445.AM2013-763A
TRANSCATHETER AORTIC VALVE IMPLANTATION WITH SAPIEN XT OR SAPIEN 3 PROSTHESES IN BICUSPID AORTIC VALVE DISEASE
Authors: Hiroyuki Kawamori, Yoshio Maeno, Yigal Abramowitz, Nobuyuki Takahashi, Yoshio Kazuno, Mohammad Kashif, Tarun Chakravarty, Wen Cheng, Raj Makkar, Hasan Jilaihawi
Journal: Journal of the American College of Cardiology (2016)