tbuckley/cabot-search huggingface dataset:
Abstract 3005: Cdca7l is a sex-specific modifier of astrocytoma and glioblastoma.
Authors: Karlyne M. Reilly, Min-Hyung Lee, Jessica Amlin-Van Schaick
Journal: Cancer Research (2013)

Abstract Astrocytoma and glioblastoma-multiforme (GBM) are the most common primary brain tumors and are currently incurable. Both astrocytoma and GBM show male-predominance, with a male-to-female ratio of 1.3:1, respectively. Previously, our lab has demonstrated that astrocytoma/GBM-tumorigenesis in the Nf1-/+;Trp53-/+cis (NPcis) mouse model shows gender-bias in certain genetic contexts. Using linkage-analysis in the NPcis mouse, we have identified a male-specific genetic modifier locus on distal chromosome 12, named Arlm1 for Astrocytoma-resistance-locus-in-males1. To identify candidate genes for the Arlm1 modifier of glioma risk, we used combinational bioinformatics approaches and cross-species comparisons, identifying Cell-division-cycle-associated-7-like (CDCA7L) as a strong candidate for a male-specific astrocytoma susceptibility gene among 503 candidate genes. To investigate the role of CDCA7L in astrocytoma/GBM, we analyzed CDCA7L in both human and mouse astrocytoma cell lines in both genders. CDCA7L expression was upregulated in astrocytoma cells compared to normal brain with male-predominance. shRNA-mediated CDCA7L-silencing in male-derived astrocytoma cells led to decrease of both cell growth and viability in both human and mouse. Further mechanistic study revealed that CDCA7L-depletion in male-derived astrocytoma cells was connected with the induction of Caspase3-activation and reduction of CyclinD1-expression, suggesting the oncogenic role of CDCA7L in astrocytoma/GBM. On the other hand, CDCA7L-overexpression in female-derived astrocytoma cells showed less degree of cell proliferation and viability increases. Strikingly, CDCA7L-depletion in human female-derived astrocytoma cell line, U87MG caused increase of both cell growth and viability. These data highlight the sex-specificity of CDCA7L in astrocytoma/GBM tumorigenesis and provide evidence for the first modifier of male-specific susceptibility to brain tumors. Citation Format: Karlyne M. Reilly, Min-Hyung Lee, Jessica Amlin-Van Schaick. Cdca7l is a sex-specific modifier of astrocytoma and glioblastoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3005. doi:10.1158/1538-7445.AM2013-3005
EVALUATION OF HEMOGLOBIN A1C AND ADMISSION BLOOD GLUCOSE AS PREDICTORS OF IN-HOSPITAL AND 12-MONTH MORTALITY IN PATIENTS WITH STEMI
Authors: Menfil A. Orellana-Barrios, Joseph W. Fries, Kenneth Nugent, Scott Shurmur, Gary Meyerrose
Journal: Journal of the American College of Cardiology (2016)
Endoscopic Ultrasonography role in the multimodality treatment of stage II-III rectal cancer: preliminary results
Authors: C Fabbri, A Maestri, AM Polifemo, F Salvi, V Cennamo, S Macchia, S Ghersi, F Ferrara, MP Foschini, G Frezza, E Jovine, ND 'Imperio
Journal: Endoscopy (2006)

Background and aims: standard treatment for stage II-III rectal cancer is a multimodality approach with primary combined chemioradiotherapy and radical surgery. It is very important, for a better planning of chemioradiotherapy, an accurate evaluation of the depth of the tumor infiltration (T-status) and of the presence of loco-regional lymph node metastasis (N-status). Endoscopic ultrasonography (EUS) is now the technique of choice for locoregional staging of rectal cancer. The purpose of this ongoing study is to present preliminary data regarding restaging using EUS in patients who underwent preoperative chemioradiotherapy.
Tissue Polypeptide Antigen Activity in Cerebrospinal Fluid: A Marker of Central Nervous System Metastases of Breast Cancer
Authors: F. Bach, G. Soletormos, P. Dombernowsky
Journal: JNCI Journal of the National Cancer Institute (1991)

Tissue polypeptide antigen (TPpA) in the cerebrospinal fluid (CSF) was measured in 59 consecutive breast cancer patients with suspected central nervous system (CNS) metastases. Subsequently, we determined that 13 patients had parenchymal brain metastases, 10 had leptomeningeal carcinomatosis, and 36 had no CNS involvement. The concentration of TPpA, which is a nonspecific marker for cell proliferation, was significantly higher in patients with CNS metastases than in those without it (P less than .0001; Mann-Whitney test). A tentative cutoff value for CNS metastases was set at 95 U/L TPpA; the upper limit of values indicating absence of CNS metastases was 89 U/L. Given these cutoff points, the sensitivity of TPpA as a marker for CNS metastases was 74% and the specificity was 100%; the predictive values of positive and negative tests were 100% and 86%, respectively. In 16 patients with CNS metastases, no correlation was found between TPpA activity in corresponding CSF and blood samples (correlation coefficient, Spearman's rho = .4; P greater than .1). In three patients treated for leptomeningeal carcinomatosis, the measurements of CSF TPpA showed correlation between the presence of tumor cells in the CSF and neurological clinical function. TPpA concentrations decreased in parallel with the clinical response and increased prior to CNS disease progression. As a marker for CNS metastases, the level of TPpA in the CSF in breast cancer patients appears to be superior to the level of protein, lactate dehydrogenase, or glucose, which showed very low sensitivity (41%, 47%, and 8%, respectively). For quantitative evaluation of treatment for leptomeningeal carcinomatosis, the TPpA level appears to be valuable and superior to CSF cytology, because tumor cells are not always present in CSF samples from patients with this condition.
Epistemology of screening for behavioral toxicity.
Authors: P B Dews
Journal: Environmental Health Perspectives (1978)

A method is described for the assay of the behavioral effects of volatile solvents on mice and illustrated with pilot results on trichlorethylene. A dose-effect curve has been determined for the effects on schedule controlled responding and compared with the dose-lethality curve and the TLV for man. The OR50 for behavioral effects was 1/5 of the LD50 and 50 times the TLV for long-term exposure of man. An analysis of the errors involved in determination of effects on whole animals leads to the conclusion that subtle effects, representing a few per cent change, will not be detectable in routine screening. It is suggested nevertheless that information on the midrange, knowable, part of the dose-effect curve may prove useful in predicting safe levels for man.
AB0200 High expression of toll like receptors 7 and interleukin 18 in lupus nephritis
Authors: F.R. Spinelli, S. Truglia, C. Alessandri, M. Bombardieri, F. Ceccarelli, F. Martinelli, F. Miranda, V. Conti, C. Perricone, C. Giannakakis, T. Faraggiana, G. Valesini, F. Conti
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Both humoral and cellular immune mechanisms are involved in the development of lupus nephritis (LN). Due to the ability to recognize microbe specific and endogenous nucleic acids, Toll-like receptors (TLRs) may contribute to renal inflammation. <h3>Objectives</h3> Aim of this study was to evaluate the expression of TLR3, TLR7, TLR8 and IL18 in the kidney of SLE patients. <h3>Methods</h3> Kidney sections have been analyzed by standard immunohistochemistry using anti-human TLR3, 7, 8 and IL-18 MoAb. Using dedicated software, all the sections were analyzed for number of nuclei per mm<sup>2</sup> positive for TLR3/7/8 and IL18. <h3>Results</h3> Table 1 shows glomerular and tubular expression of TLRs and IL18 in 20 kideny sectiona from LN patients. No difference in glomerular and tubular expression of TLRs and IL18 among histological classes was observed; only glomerular TLR3 was higher in class III than in IV (p=0.004). In the whole population and in classes III and IV, glomerular and tubular expression of TLRs did not differ one from the other; as for IL18, glomerular expression was higher than the tubular one (p=0.03) in class IV. Considering all the biopsies, both tubular and glomerular TLR7 expression was significantly higher than TLR3 and TLR8 expression (p&lt;0.005). In class III, glomerular and tubular TLR3 was higher than TLR8 (p=0.0005 and p=0.004); glomerular and tubular TLR7 was higher than TLR8 (p=0.009 and p=0.006). In class IV glomerular and tubular TLR7 was higher than TLR3 (p=0.02 and p=0.03) and TLR8 (p=0.0009 and p=0.002). <h3>Conclusions</h3> We observed a high number of nuclei expressing IL18 and TLR7, a moderate number expressing TLR3 and a low number expressing TLR8 in tubules and glomeruli of LN patients. This data reinforces the hypothesis that activation by endogenous TLRs ligands may contribute to the persistent expression of proinflammatory cytokines in SLE patients. <h3>Disclosure of Interest</h3> None Declared
Stat Bite: Knowledge and Use of Colorectal and Prostate Cancer Screening
Authors: A. Potosky
Journal: JNCI Journal of the National Cancer Institute (1991)

Journal Article Stat Bite: Knowledge and Use of Colorectal and Prostate Cancer Screening Get access Arnold Potosky Arnold Potosky Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 83, Issue 15, 7 August 1991, Page 1051, https://doi.org/10.1093/jnci/83.15.1051 Published: 07 August 1991
OC-015 High Grade Dysplasia Arising In Barrett’s Oesophagus Can Be Accurately Diagnosed Combining Fourier Transform Infrared Spectroscopy And Single Element Attenuated Total Reflectance Ftir Data
Authors: L Foreman, RJ Haidry, M Banks, M Novelli, M Rodriguez-Justo, J Dougan, S Kazarian, P Rich, L Lovat
Journal: Gut (2014)

<h3>Introduction</h3> Fourier transform infrared spectroscopy (FTIR) detects specific molecules in human tissue from unique vibrational absorption spectra in the mid Infra-Red region (1800–900 cm<sup>-1</sup>). Single element Attenuated total reflectance (ATR) contains averaged FTIR spectra from the superficial 10 microns of an entire oesophageal biopsy. We aim to extract individual cellular characteristics with molecular resolution by using FTIR and ATR on ex-vivo oesophageal biopsy specimens from patients undergoing endoscopy for BE surveillance or neoplasia assessment to detect high grade dysplasia (HGD). <h3>Methods</h3> 731 spectra of 374 fresh biopsies from 76 patients were analysed. Biopsies were taken from visible BE. Before being placed in formalin, they were analysed by a spectrometer fitted with liquid nitrogen-cooled detector and ATR silicon microprism. For each spectrum 500 interferograms were averaged before Fourier transformation. Spectra were pre-processed using MATLAB scripts by spectrally removing liquid water and water vapour contributions, vector normalising to the 1610–900 cm<sup>-1</sup> region and second derivative conversion to remove baseline artefacts. Specific cellular characteristics were first determined. Unstained 8 µm tissue sections from 1 patient were analysed with FPA (Focal Plane Array)-FTIR imaging and correlated with stained slides. It was possible to accurately describe specific features ofsquamous epithelium (SQ), columnar lined epithelium (CLE), and lamina propria (LP) with this method. These features were applied to the 374 fresh biopsies using ATR-FTIR. Combined clustering and partial least squares regression discrimination (PLSDA) was used to build a diagnostic pipeline. Biopsies were grouped according to their cellular characteristics from the prior FTIR imaging. (1. SQ vs Rest, 2. SQ only biopsies, 3. CLE only biopsies, 4. CLE and LP containing biopsies and 5. LP containing biopsies only). <h3>Results</h3> We distinguished SQ mucosa from CLE (BE), HGD and OAC tissue at an overall sensitivity of 89% and specificity of 91%. By grouping the spectra into groups according to their cellular contents, HGD was distinguished from all other biopsies with sensitivities and specificities of 68 and 89% (CLE only), 74 and 82% (CLE and LP) and 94 and 97% (LP only) respectively. <h3>Conclusion</h3> Combined FTIR and ATR-FTIR spectroscopy can accurately distinguish HGD arising in BE on ex-vivo biopsy specimens and might become accurate enough to exclude routine histopathological evaluation in these patients. <h3>Disclosure of Interest</h3> None Declared.
OC-065 Audit of Radiation Exposure in Crohn’S Past and Present: Abstract OC-065 Table 1
Authors: S Savva, E Rowland, B Rathbone
Journal: Gut (2013)

<h3>Introduction</h3> The aim of this audit was to establish how much radiation patients with newly diagnosed Crohn’s disease are exposed to and if this has changed over time within our practise. We hoped that with the increased availability of new imaging modalities such as ultrasound and MRI scanning the exposure of these patients to radiation may have fallen. <h3>Methods</h3> Using the IBD database all Crohn’s patients diagnosed from 1995 to 2011 were identified. We then collected data on 59 patients diagnosed from 1995 to 2001 and on 61 patients diagnosed from 2006 to 2011. These dates were chosen as ultrasound of the small bowel was introduced in UHL in 2002, and took a few years to become established as an imaging technique in Crohn’s. Data for each patient was collected only on studies performed in the first year of diagnosis, to prevent bias occurring due to the length of time that patients had the disease. We also felt the first year was likely to encompass studies performed to establish the diagnosis and extent of disease. <h3>Results</h3> For patients diagnosed between 1995 and 2001 with Crohn’s disease the average exposure to radiation in the first year was 1.83 mSv (range 0–12.3 mSv). For patients diagnosed between 2006 and 2011 we found an average exposure of 2.67 mSv (range 0–24 mSv), an increase of 46%. <h3>Conclusion</h3> Radiation exposure in Crohn’s disease appears to be increasing despite new modalities such as ultrasound and MRI. The increase is attributed to the increased use of CT scanning, as availability and accuracy of imaging via CT in Crohn’s disease have improved in recent years. With the gradual introduction of low-dose CT scanning, we would hope these levels will fall again in the near future. Furthermore we observed an increase in the use of plain abdominal films of 87.5%. We feel this may be attributable to the shift in attitude towards treating unwell patients with the increasingly effective and available pharmacological therapies, rather than surgical options, although further audit should be carried out to establish if this is indeed the case. <h3>Disclosure of Interest</h3> None Declared
P580 NONINVASIVE BLOOD FIBROSIS INDICES PREDICT INTRAHEPATIC RECURRENCE AFTER CURATIVE RESECTION OF HEPATOCELLULAR CARCINOMA
Authors: W.-M. Choi, J.-H. Lee, Y.Y. Cho, M. Lee, J.-J. Yoo, Y. Cho, D.H. Lee, Y.B. Lee, S.J. Yu, Y.J. Kim, J.-H. Yoon, C.Y. Kim, H.-S. Lee
Journal: Journal of Hepatology (2014)
Selective viral immunosuppression of the graft-versus-host reaction.
Authors: T J Romano, M Nowakowski, B R Bloom, G J Thorbecke
Journal: The Journal of Experimental Medicine (1977)

Graft-vs.-host (GVH) reactivity of parental lymph node (LN) cells was assayed by measurements of 3H-thymidine incorporation in vivo in spleens of irradiated F1 recipients. Preincubation of parental LN cells with vesicular stomatitis virus (VSV) for 2 h at 37 degrees C followed by washing resulted in an 85-90% reduction in splenic radioactivity, as did injection of VSV on days 0-2 after recipients received untreated parental LN cells. In contrast, 3H-thymidine incorporation in the spleens or irradiated F1 hosts was not affected by VSV when F1 bone marrow cells were incubated with the virus. In addition, preincubation of F1 B cells with VSV still allowed these syngeneic B cells to be recruited into proliferation by mitomycin-treated parental LN cells. The inhibitory effect of VSV, thus, seems to be specific for T-cell proliferation. These observations suggest that viral immunosuppression might be capable of being developed into a useful strategy for selective deletion of lymphocytes capable of reacting against histocompatibility antigens and initiating GVH reactions.
Abstract 4428: Diethylnitrosamine-induced hepatocarcinogenesis is suppressed in mice lacking hepatic retinoid storage
Authors: Yohei Shirakami, William S. Blaner, Masahito Shimizu, Hisataka Moriwaki
Journal: Cancer Research (2012)

Abstract Retinoids are known to possess anti-cancer effects and a clinical trial of a synthetic retinoid for hepatocellular carcinoma (HCC) is ongoing recently in Japan. Loss of retinoid-containing lipid droplets upon hepatic stellate cell (HSC) activation is one of the first events in the development of liver disease leading to hepatocellular carcinoma (HCC). Although retinoid stores are progressively lost from HSCs during the development of hepatic disease, how this affects hepatocarcinogenesis is unclear. To investigate this, we used diethylnitrosamine (DEN) to induce hepatic tumorigenesis in matched wild-type (WT) and lecithin:retinol acyltransferase (LRAT) knockout (KO) mice, which lack stored retinoid and HSC lipid droplets. Male 15 day-old WT or Lrat KO mice were given intraperitoneal injections of DEN (25 mg/kg body weight). Eight months later Lrat KO mice showed significantly less liver tumor development compared to WT mice, characterized by less liver tumor incidence and smaller tumor size. Two days after DEN injection, lower serum levels of alanine aminotransferase and decreased hepatic levels of cyclin D1 were observed in Lrat KO mice. Lrat KO mice also exhibited increased levels of retinoic acid-responsive genes, including p21, lower levels of cytochrome P450 enzymes required for DEN-bioactivation, and higher levels of the DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT), both before and after DEN treatment. Our results indicate that Lrat KO mice are less susceptible to DEN-induced hepatocarcinogenesis due to increased retinoid signaling and higher expression of p21, which is accompanied by altered hepatic levels of DEN-activating enzymes and MGMT in Lrat KO mice also contribute to decreased cancer initiation and suppressed liver tumor development. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4428. doi:1538-7445.AM2012-4428
Endoscopic ultrasound (EUS) guided cyanocrylate injection, an accurate and safe method for the treatment of gastric varices
Authors: F Junquera
Journal: Endoscopy (2011)

Therapy of gastric varices (GV) is challenging. Endoscopic injection of cyanoacrylate has been a step forward in their treatment. However, it may be troublesome when massive bleeding occurs and, is associated to severe complications, both systemic (embolism) and local (hemorrhage from ulcers). EUS-guided injection of cyanoacrylate in the perforating veins feeding the GV may offer some advantages: minimize the risk of complications due to the low volume of injection needed, its application is independent of endoscopic view, avoiding gastric wall injection, and display the complete occlusion of GV.
AB0084 Serum Levels of Syndecan-1 and Organ Involvement in Systemic Lupus Erythematosus
Authors: N. Fajardo-Robledo, V. Diaz-Rizo, A. Rocha-Muñoz, J. Muñoz-Valle, L. Gonzalez-Lopez, J. Gamez-Nava
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Syndecan-1 is expressed on plasma cells surface and serum levels as reflect an activation of these cells. To date, a significant association between serum levels of syndecan-1with disease activity has been observed in a study evaluating patients with systemic lupus erythematosus (SLE). Nevertheless, to date there is a lack of information about. <h3>Objectives</h3> Evaluate the association of syndecan-1 serum levels with disease activity and clinical variables <h3>Methods</h3> We included 46 patients with SLE and were compared with 46 controls. Disease characteristics and treatments were assessed in patients with SLE. Serum levels of syndecan-1 and BLyS were obtained and correlated with clinical variables of the SLE patients. <h3>Results</h3> Patients with SLE had a mean age of 42 years. From 46 patients with SLE, 30 (70%) had disease activity with a SLEDAI &gt;3, and 21 (47%) had renal involvement. Patients with renal involvement had a trend to higher serum levels of syndecan-1 (129.7±80.4 vs. 87.1±65.4 respectively, p=0.07) Syndecan-1 correlated significantly with proteinuria (r=0.39, p=0.01). Also patients with cutaneous involvement had higher levels of syndecan-1 compared with patients without this involvement (126.5±81.3 vs. 96.2±69.2 respectively, p=0.01). Patients with higher levels of syndecan-1 also had a trend to higher doses of prednisone (r=0.28, p=0.07), and higher doses of mofetil mycophenolate (r=0.29, p=0.06) reflecting the treatment for a more severe disease activity. <h3>Conclusions</h3> These results suggest that syndecan-1 serum levels are related with disease activity, particularly renal involvement. The potential role of this molecule should be evaluated in a prospective cohort study in order to identify if could be useful as a marker for different therapeutic outcomes in SLE. This project was financed by a grant of Instituto Mexicano del Seguro Social: FIS/PROT/G13/1202. <h3>References</h3> Sanderson RD, Lalor P, Bernfield M. B lymphocytes express and lose syndecan at specific stages of differentiation. Cell Regul. 1989 Nov;1(1):27-35. Minowa, K., H. Amano, S. Nakano, S. Ando, T. Watanabe, Y. Nakiri, E. Amano, Y. Tokano, S. Morimoto, and Y. Takasaki, Elevated serum level of circulating syndecan-1 (CD138) in active systemic lupus erythematosus. Autoimmunity, 2011. 44(5) <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5962
PROGNOSTIC SIGNIFICANCE OF SERUM OSTEOPROTOGERIN LEVELS IN PATIENTS WITH CORONARY ARTERY DISEASE
Authors: Evangelos Oikonomou, Gerasimos Siasos, Theodoros Zografos, Marina Zaromitidou, Konstantinos Maniatis, Sotirios Tsalamandris, Nikolaos Papageorgiou, Manolis Vavuranakis, Nikolaos Gouliopoulos, Konstantinos Zisimos, Vasiliki Tsigkou, Athanasios Papavassiliou, Christodoulos I Stefanadis, Dimitris Tousoulis
Journal: Journal of the American College of Cardiology (2015)
Colonic xanthoma: a rare non-neoplastic polypoid lesion
Authors: Masaki Katsurahara, Noriyuki Horiki, Shunsuke Tano, Yasuhiko Hamada, Kyosuke Tanaka, Hiroyuki Inoue, Yoshiyuki Takei
Journal: Endoscopy (2014)

A 78-year-old man with a history of hyperlipidemia underwent colonoscopy because of a positive fecal occult blood screening test without any symptoms. In the sigmoid colon, a whitish-yellow, sessile, polypoid lesion was found, which measured about 15 mm in diameter ([Fig. 1]). Magnifying endoscopy with narrow band imaging revealed a regular arrangement of round pits and previously invisible microvessels on the surface of the mucosa ([Fig. 2]).
Book: Medicines Management
Authors: N Barber
Journal: BMJ (1999)

BMJ Books, £19.95, pp 196 ISBN 0 7279 1274 7 Rating: ![Graphic][1]</img>![Graphic][2]</img>![Graphic][3]</img> About £1 of every £7 that the NHS spends goes on drugs, and spending on drugs is rising rapidly. There is nothing new in this—in the 1920s the chief medical officer was making similar complaints about the expenditure on drugs. What is new is the complex and interweaving set of activities designed to manage medicines and, hence, expenditure. In Britain this extends from policy level restrictions of … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif