tbuckley/cabot-search huggingface dataset:
Abstract 4083: Long stress-responsive non-coding transcripts identified in a whole genome screen have altered expression in cancer
Authors: Jessica M. Silva, Damon S. Perez, Jay Pritchett, Merideth Halling, David I. Smith
Journal: Cancer Research (2010)

Abstract The number of non-coding transcripts (NCTs) exceeds the number of transcripts that code for protein. Their diverse and important functions are now being identified and utilized in the lab as well as in the clinic. Utilizing the Affymetrix Gene Chip Human Tiling 1.0R Array Set, we analyzed the transcription across the entire genome in both normal human bronchial epithelial cells (NHBE) alone and cells exposed to the tobacco carcinogen (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone), NNK. We focused our efforts on the identification and function of novel long non-coding transcripts that had increased expression after exposure to NNK. We report on the identification of 12 long NNK-induced non-coding transcripts (NiTs) that are 300 nucleotides and larger in length. We first validated that these transcripts were indeed stress responsive using quantitative real-time RT-PCR (qPCR) and by Northern blot analysis revealed that these transcripts were between two and four kilobases in length. We also show using qPCR that these transcripts are more abundantly expressed in normal rapidly growing tissues or tissues that are under cellular stress. We show that there is altered expression of NiTs in a panel of lung cancer cell lines and many of these transcripts had increased expression in many breast cancer cell lines. We further analyzed one of these transcripts, NiT5 and identified it to be a 2.5 kb antisense, polyadenylated transcript by RLM-RACE and by Northern Blot analysis. Isolating nuclear and cytoplasmic fractions in BT474 cells, we identified Nit5 to be localized in the nucleus and found it to be transcribed by RNA polymerase III. NiT5 also has increased expression in breast, ovarian and cervical cancer-derived cell lines relative to normals from these three sites, but not increased expression in lung cancer cell lines. In summary, our results suggest that these novel long non-coding transcripts play an important role in stress also may play a role in cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4083.
Case control study of hormone replacement therapy (HRT) and breast cancer in Japanese women
Authors: M. Takeuchi, T. Saeki, M. Sano, H. Sonoo, H. Honjo, K. Ochiai, H. Sugano, Y. Komiike
Journal: Journal of Clinical Oncology (2006)

10012 Background: Some prospective studies in US and UK (represented by Women’s Health Initiative and Million Women Study) show that HRT use may increase the risk of breast cancer. However, it is unclear whether or not this perspective is applicable to Japanese women, due to several factors such as the difference in incidence of breast cancer, difference in HRT prevalence, which are to be concerned in this study. To investigate the relationship between HRT use and breast cancer, a case-control study was conducted in Japan. Methods: We sent self-administered questionnaire to 4,500 cases who have a previous history of confirmed histological breast cancer and 4,500 controls who were selected according to the inclusion criteria. All the subjects were between age 45 and 69 at the time of enrollment. Among these subjects, 3,434 in case group and 2,427 in control group sent back their questionnaire (total 5,861). The datacenter was run in Kitasato Univ. and the statistical analysis was performed using SAS (version 9.1). Control was selected by considering the screening record for GI disease and respiratory disorders, with no previous history of breast cancer, gynecological and hormonal disease at the time of screening. We asked about past history of exposure to the factors supposed to be breast cancer-causing; previous or current use of HRT, age at diagnosis, academic background, BMI, lifestyle habits, age of menarche, birth history, history of breast feeding, family history, use of contraceptive agents and menopausal status. Results: As the main analysis result, 164 (5.0%) out of 3,316 cases and 253 (7.4%) out of 2355 controls had used HRT (Odds ratio 0.432 [95% CI 0.352–0.530]. Conclusions: The result of this study shows that HRT users were less likely to develop breast cancer than never users. However, in this study, there was a difference in the observation period for the past use of HRT between cases and controls. Considering the transition of HRT prevalence in Japan, there may be a possibility that this difference can be a cause of a serious bias for the main analysis result. Further sensitivity analyses are needed to evaluate the robustness of the findings and this point is under consideration. No significant financial relationships to disclose.
302MiR-126-5p promotes endothelial cell proliferation and limits lesion formation during atherosclerosis by suppressing Dlk1
Authors: M Nazari Jahantigh, Y Wei, K Heyll, J Corbalan Campos, J Grommes, S Wang, E Olson, C Weber, A Schober
Journal: Cardiovascular Research (2014)

Endothelial cell (EC) function and survival are affected by hyperlipidemia and disturbed laminar flow, which in turn leads to atherosclerosis; endothelial miR-126-3p promotes angiogenesis and reduces atherosclerosis. We have previously reported that the passenger strand miR-126-5p reduces neointima formation by increasing EC proliferation through suppression of Dlk1 following endothelial denudation. Here we aimed to assess the role of the miR-126-5p/Dlk1 axis in EC proliferation during atherogenesis.
Reversible Loss of Vision in Malignant Hypertension
Authors: Dhananjay Shukla, Asif Y Virani
Journal: New England Journal of Medicine (2016)

A 35-year-old man presented with reduced vision in both eyes. He had no relevant medical history apart from intermittent headaches. Fundus examination revealed bilateral disk edema, macular edema with lipid exudates, splinter hemorrhages, and cotton-wool spots.
Soft Tissue Uptake Observed on Tc-99m MDP Bone Scans
Authors: ESER LAY ERGÜN, EMEL CEYLAN
Journal: Clinical Nuclear Medicine (2001)

From Hacettepe University Medical Faculty, Department of Nuclear Medicine, Ankara, Turkey Received for publication December 28, 2000. Revision accepted March 12, 2001. Correspondence: Dr. Eser Lay Ergün, Hacettepe Üniversitesi Tıp Fakültesi, Nükleer Tıp A.B.D., 06100 Sıhhıye, Ankara, Turkey.
Abstract 5293: Prognostic significance of miR-194 in endometrial cancer.
Authors: Haiyan Zhai, Mihriban Karaayvaz, Peixin Dong, Noriaki Sakuragi, Jingfang Ju
Journal: Cancer Research (2013)

Abstract Endometrial cancer (EC) is the leading malignant tumor occurring in the female genital tract and some subtypes are highly invasive and metastatic. miRNAs are small non-coding RNAs that have a broad impact on cancer progression. In particular, miR-194 regulates epithelial to mesenchymal transition (EMT) by suppressing the expression of BMI-1 in EC. In this retrospective study, the clinical significance of miR-194 was investigated in archival EC specimens. We extracted total RNA from thirty-two EC samples and quantified the expression level of miR-194. We discovered that the expression level of miR-194 was significantly (P = 0.03) lower in type I EC patients with more advanced stage. In addition, patients with higher miR-194 levels have better prognosis than those with lower miR-194 levels (P = 0.0067; Cut-off value of miR-194 = 0.3). These results indicate that miR-194 has potential to serve as prognostic biomarker for EC patients. Citation Format: Haiyan Zhai, Mihriban Karaayvaz, Peixin Dong, Noriaki Sakuragi, Jingfang Ju. Prognostic significance of miR-194 in endometrial cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5293. doi:10.1158/1538-7445.AM2013-5293
Ga-67 Scan To Detect Intra-abdominal Infection in Patients After Colorectal Surgery
Authors: S C Tsai, T H Chao, W Y Lin, S J Wang
Journal: Clinical Nuclear Medicine (2001)

Purpose Intra-abdominal infection is still an important issue in colorectal surgery. The Ga-67 scan is relatively inexpensive, but the delay in diagnosis and the intense physiologic bowel activity are considerable drawbacks to its use in abdominal investigations. In this study, the authors performed 24-hour Ga-67 scans and read the images with the surgeon to detect abdominal infection in patients who underwent colorectal surgery. Methods Sixty-one patients who underwent colorectal surgery were enrolled in this study. Ga-67 scintigraphy was performed 24 hours, and later as needed, after the injection of 111 MBq (3 mCi) Ga-67 citrate. All scans were interpreted together by a nuclear medicine specialist and the surgeon. The intensity of Ga-67 uptake was recorded as grade 0: no uptake; grade 1: less than liver uptake; grade 2: equal to liver uptake; or grade 3: greater than liver uptake. When the intensity of Ga-67 uptake was equal to or greater than grade 2, the pattern of Ga-67 uptake was classified as changing or unchanging. A positive Ga-67 scan was defined as having an intensity of grade 2 or 3 with an unchanging pattern. Results Nineteen abdominal infections were diagnosed, all by Ga-67 scans, for a diagnostic sensitivity rate of 100%. Of the 42 patients with no intra-abdominal infection, only 2 had positive Ga-67 scans. This resulted in a specificity rate of 95.2%. The overall accuracy of the Ga-67 scan in the detection of intra-abdominal infection after colorectal surgery was 96.7%. Conclusions The Ga-67 scan is useful for detecting intra-abdominal infection after colorectal surgery. Reading the images with the surgeon and using a lateral view can decrease the false-positive rate. A negative Ga-67 scan can rule out intra-abdominal infection, whereas a positive Ga-67 scan indicates the need for further patient evaluation after colorectal surgery.
824 IMPACT OF IL28B GENOTYPE ON HCV TREATMENT DECISION IN A LARGE FRENCH COHORT
Authors: C. Renou, T. Allègre, D. Ouzan, M. Bourlière, P. Delasalle, A. Lafeuillade, J.-F. Cadranel, N. Haddad, T. Asselah, G. Pénaranda, F. Sauser-Chirat, H. Khiri, P. Halfon
Journal: Journal of Hepatology (2013)
Ga-67 Scan in the Diagnosis and Monitoring of Ludwig’s Angina
Authors: REN-SHYAN LIU, YUM-KUNG CHU, LEE-SHING CHU, CHENG-PEI CHANG, SU-QUINN LIAO
Journal: Clinical Nuclear Medicine (2001)

LIU, REN-SHYAN M.D.; CHU, YUM-KUNG M.D.; CHU, LEE-SHING M.D.; CHANG, CHENG-PEI M.D.; LIAO, SU-QUINN M.D. Author Information
The academic dilemma of the inpatient unit director
Authors: E Leibenluft, P Summergrad, A Tasman
Journal: American Journal of Psychiatry (1989)

Inpatient units in academic departments are typically directed by junior faculty members, who quickly abandon these positions for less demanding, more rewarding jobs. These frequent turnovers in the directorship compromise the clinical, research, and educational functions of the inpatient unit. The authors believe that the average inpatient director's truncated term can be traced to two causes: an exacerbation of the junior faculty member's developmental crisis by factors intrinsic to the inpatient unit and a disparity between the expectations for academic productivity and the opportunities for scholarly activity. These conflicts are elaborated and pragmatic ways of relieving this situation are suggested.
AB0730 Kikuchi-fujimoto disease: Past and future dangerous liaisons:
Authors: V. Ortiz-Santamaria, B. Consola, J. Sanchez-Manubens, M.T. Coll, M. Bernet, A. Villagrasa, J. Cuquet, E. Llargués, X. Surís
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Kikuchi-Fujimoto disease (KFD) is known as histiocytic necrotizing lymphadenitis. The most important signs and symptoms are cervical lymph node enlargement and fever. This disease is usually benign and self-limiting. The cause of this disease is not known, although infectious and autoimmune etiologies such as Systemic Lupus Erythematosus (SLE) have been proposed. <h3>Objectives</h3> To report clinical manifestations, laboratory findings and follow-up of patients with Kikuchi-Fujimoto disease. <h3>Methods</h3> We report 4 cases of KFD collected in a county hospital. The observation period was 1990-2010. The reference area had a population of 300.000 inhabitants. We analyzed the clinical features, laboratory findings, clinical course and its relation to SLE. <h3>Results</h3> The cases of KFD included 4 females with an average age of 23 years old (13-40) at the time of the diagnosis (see Table). One of the patients (case 3) presented at an unusually young age with atypical lymph-node localization: supraclavicular, inguinal and axillary. During follow-up, we confirmed the diagnosis of SLE in 2 patients. These diagnoses changed the therapeutic strategy in these cases. The other 2 cases are being monitored closely, due to the high probability that they will develop an autoimmune disease. <h3>Conclusions</h3> The importance of a diagnosis of KFD lies in the possible association with an autoimmune disease. KFD can precede, coincide or appear during the development of autoimmune diseases, usually SLE. A diagnosis of KFD requires histological confirmation. <h3>Disclosure of Interest</h3> None Declared
PTU-002 The hepatic inflammatory milieu of acetaminophen induced acute liver falure induces alternatively activated, M2-like, macrophages
Authors: C G Antoniades, V Zingarelli, L Possamai, W Khamri, N Vergis, R Mitry, A Quaglia, W Bernal, G Auzinger, Y Ma, J Wendon, M Thursz
Journal: Gut (2012)

<h3>Introduction</h3> Human acetaminophen-induced acute liver injury (AALF) is characterised by areas of hepatic necrosis that are infiltrated by macrophages (mϕ) and elevated concentrations of anti-inflammatory cytokines. Anti-inflammatory mediators, such as secretory leucoprotease inhibitor (SLPI) and IL-10 contribute to the functional switching of mϕ from a proinflammatory (M1) type to an alternatively activated (M2) phenotype that promotes tissue repair processes. We sought to determine the effects of the hepatic inflammatory milieu on peripheral monocytes (MO) and MO derived mϕ in AALF. <h3>Methods</h3> Phosphoflow technique was used to evaluate NF-kBp65, STAT-3 signalling pathways in ex-vivo MO in 10 AALF patients and 10 healthy controls (HC). Results expressed as MFI and ratio of activation. Regional changes (portal vein [PV]), hepatic vein [HV])) were assessed in 5 AALF patients at time of transplantation. Serum (AALF [n=34]; HC [n=15]), hepatic (AALF [n=7]; HC [n=8]) and regional levels of TNF-α, IL-10 and SLPI were measured. The effect of the hepatic microenvironment was assessed in five cell culture experiments: purified CD14+ MO from HC were incubated with homogenates from AALF and culture medium (CM). <h3>Results</h3> In MO, TLR-4 stimulation reduced NF-kBp65 expression in AALF compared to HC (0.8 vs 1.6; p=0.001). Ex-vivo STAT-3 expression was significantly elevated in AALF patients compared to HC (600 vs 232; p=0.02). AALF patients had higher serum concentrations of IL-10 (170 vs 40; p&lt;0.02), SLPI (71200 vs 43310; p&lt;0.0001) compared to HC. A trans-hepatic (HV&gt;PV) gradient was seen for IL-10 and SLPI but not for TNF-α. Hepatic levels of IL-10 (2 vs 0.6; p&lt;0.02) and SLPI (442 vs 116; p&lt;0.01) were significantly elevated in AALF compared to HC tissue, with peak concentrations of IL-10 detected in necrotic areas while SPLI was highest in areas of hepatic regeneration. In vitro exposure to AALF milieu induced a unique CD14+CD16+ macrophage phenotype characterised increased expression of alternative (M2) activation markers- CD36 (78 vs 55%; p=0.01) and CD163 (58 vs 32.5%; p=0.04), reduced LPS-induced TNF-a (19.8 vs 40.5%; p=0.02), IL-6 (13.2 vs 22%; p=0.04) and enhanced phagocytosis (80.5 vs 70%; p=0.03) when compared to MO incubated CM. <h3>Conclusion</h3> In AALF, circulating monocytes show modulations in intracellular signalling pathways compatible with anti-inflammatory responses. Our data also indicate that the anti-inflammatory hepatic microenvironment preferentially induces alternatively activated (M2)-like macrophages that may be implicated in resolution of acute liver injury. <h3>Competing interests</h3> None declared.
Re: Blood Levels of Organochlorine Residues and Risk of Breast Cancer
Authors: Matthew P. Longnecker, Stephanie J. London
Journal: JNCI Journal of the National Cancer Institute (1993)

Journal Article Re: Blood Levels of Organochlorine Residues and Risk of Breast Cancer Get access MATTHEW P. LONGNECKER, MATTHEW P. LONGNECKER Search for other works by this author on: Oxford Academic PubMed Google Scholar STEPHANIE J. LONDON STEPHANIE J. LONDON Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 85, Issue 20, 20 October 1993, Page 1696, https://doi.org/10.1093/jnci/85.20.1696 Published: 20 October 1993
Modification of spectral features by nonhuman primates
Authors: Daniel J Weiss, Cara F Hotchkin, Susan E Parks
Journal: Behavioral and Brain Sciences (2014)

Abstract Ackermann et al. discuss the lack of evidence for vocal control in nonhuman primates. We suggest that nonhuman primates may be capable of achieving greater vocal control than previously supposed. In support of this assertion, we discuss new evidence that nonhuman primates are capable of modifying spectral features in their vocalizations.
Hereditary Adult-Onset Leukodystrophy Simulating Chronic Progressive Multiple Sclerosis
Authors: Roswell Eldridge, Christo P. Anayiotos, Sandra Schlesinger, David Cowen, Christopher Bever, Nicholas Patronas, Henry McFarland
Journal: New England Journal of Medicine (1984)

We studied a large kindred with a chronic progressive neurologic disorder affecting at least 10 men and 11 women in four generations in a pattern compatible with autosomal dominant inheritance. In 20 of the affected subjects, evaluated before the availability of computerized tomography and without regard to family history, the diagnosis was multiple sclerosis. Symptoms of the neurologic disorder begin in the fourth and fifth decades and include cerebellar, pyramidal, and autonomic abnormalities. The autonomic symptoms, which involve bowel and bladder regulation and orthostatic hypotension, may be the earliest changes but are frequently disregarded. Survival for 20 years after onset is common. The CT scan is striking and shows a symmetrical decrease in white-matter density, beginning in the frontal lobes but extending to all of the centrum ovale and the cerebellar white matter. Limited pathological observation reveals gross white-matter degeneration with microscopic vacuolation, preservation of U fibers and cortical structures, and no inflammatory changes or reactive gliosis. Because of its hereditary basis, the disorder should be susceptible to genetic definition and ultimately to treatment or prevention.
Abstract 2979: Tumor suppressor ING1b maintains genomic stability through PCNA monoubiquitination and histone H4 acetylation
Authors: Ronald PC Wong, Hanyang Lin, David W. Chen, Gang Li
Journal: Cancer Research (2010)

Abstract Unrepaired lesion on DNA template blocks replication fork progression and causes replication stress. Prolonged stalling of replication may give rise to serious consequences such as replication collapse, DNA double strand breaks (DSBs), recombination and genomic instability. The lesion bypass pathway, which is regulated by PCNA monoubiquitination, is essential for resolving replication stalling. We demonstrated a novel tumor suppressive function of p33ING1b (ING1b) in preserving genomic stability upon replication stress. We found that ING1b knockdown cells exhibit defects in S phase recovery and enhanced genomic instability due to UV-induced replication blockage. We showed that ING1b is required for PCNA monoubiquitination upon replication stress. Enhanced PCNA monoubiquitination due to overexpression of the E3 ligase Rad18 or knockdown of the deubiquitinating enzyme USP1 is abrogated in ING1b depleted cells. Furthermore, ING1b is required for Rad18 foci formation, localization to chromatin and interaction with PCNA after UV. In addition, ING1b is required for foci formation of Polη, a translesion DNA polymerase, indicating that ING1b is involved in regulation of the lesion bypass mechanism. We found that ING1b depleted cells display hypoacetylation of histone H4 at S phase and treatment with class I and II histone deacetylase inhibitors restores histone H4 acetylation and PCNA monoubiquitination in ING1b knockdown cells. These data suggest that ING1b regulates PCNA monoubiquitination through histone H4 acetylation to facilitate lesion bypass and recovery from replication blockage. This provides insights into a novel mechanism regulating lesion bypass through chromatin remodeling in maintaining genomic stability upon replication stress. It has implications in developing new strategies for cancer prevention and treatment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2979.
SP0058 Doctor-Patient Communication
Authors: R. Alten
Journal: Annals of the Rheumatic Diseases (2014)

<h3></h3> The World Health Organisation has stated that patient involvement in their health care is not only desirable but a social, economic and technical necessity Patients want to be involved in the decision making process, especially when alternative treatments exist. Patients have ultimate responsibility for many decisions taken in connection with their health. Specifically, they decide when to seek medical advice, whether to accept that advice and ultimately whether to comply with prescribed medicines or whether to present a case for an alternative product. Patients associate their satisfaction with treatment with the quality of communication with their HCP Good communication is considered to be a prerequisite for a treatment to work Patients wanted to be accepted as experts on their own bodies, and expected all clinicians to be experts on RA. This made it possible for patients to "take charge" of their life situation Participant in focus group interviews identified their needs to live with a rheumatic disease. These could be classed into the following groups: Education Self-management support Emotional support They also expressed what they expected from healthcare, resulting in a fourth theme – well organised care. Patients rank physical well-being, functional well-being and psychological well-being as the most important outcomes. They want a quality of existence comparable to a status before start of their disease <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.6208
THE SEVERITY OF FUNCTIONAL MITRAL REGURGITATION ASSESSED BY THREE-DIMENSIONAL ECHOCARDIOGRAPHY: NEW CUT-OFFS ARE NEEDED
Authors: Sorina Mihaila, Denisa Muraru, Patrizia Aruta, Marcelo Haertel Miglioranza, Sabino Iliceto, Dragos Vinereanu, Luigi Badano
Journal: Journal of the American College of Cardiology (2015)