tbuckley/cabot-search huggingface dataset:
Abstract 3742: The influence of masculinity, racism, and social support on colorectal cancer screening uptake among African American men: A systematic review
Authors: Charles R. Rogers, Jamie A. Mitchell, Gabriel J. Franta, Margaret J. Foster, Deirde Shires
Journal: Cancer Research (2015)

Abstract BACKGROUND: Colorectal cancer (CRC) is both preventable and highly treatable, yet incidence and mortality rates are 25% and 50% higher among African American men compared to their white counterparts. This disproportionate burden places African American men last among all racial/ethnic groups of both genders for age-adjusted CRC mortality rates and five-year survival rates. These disparities are well documented, yet preventive screening completion among African American men is relatively low, understudied, and poorly explicated. A growing body of literature indicates that separately, masculinity, racism, and social support are strong influences on CRC screening uptake. METHODS: We conducted a systematic review with the purpose of identifying and synthesizing the connections between extant literature on the associations between masculinity, racism, social support, and CRC screening completion. Utilizing Garrard's Matrix Method, 19 studies met inclusion/exclusion criteria; and the methodological quality of the reviewed evidence was assessed. CONCLUSIONS: Thirteen studies employed non-experimental research designs; a quasi-experimental design was present in four, and two utilized experimental designs. Studies were published between 2000 and 2014; the majority within five years. Preliminary qualitative synthesis suggests that social support was most frequently addressed (79%), while masculinity and racism were equally examined (11%) for their influence on CRC screening. After evaluating the conceptual and methodological characteristics of included studies, 42% fell below average in quality and rigor. The need for increased attention to the sociocultural correlates of CRC screening for African American men are highlighted in this systematic review, and important recommendations for research and practice are provided. Citation Format: Charles R. Rogers, Jamie A. Mitchell, Gabriel J. Franta, Margaret J. Foster, Deirde Shires. The influence of masculinity, racism, and social support on colorectal cancer screening uptake among African American men: A systematic review. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3742. doi:10.1158/1538-7445.AM2015-3742
338. Improving Virus-Free Modification of Endothelial Cells - Magnetically Targeted Manipulation
Authors: Natalia Voronina, Heiko Lemcke, Frank Wiekhorst, Jens Kühn, Robert David, Gustav Steinhoff
Journal: Molecular Therapy (2016)

Regulation of angiogenesis is recognized as a crucial component in the treatment of multiple diseases, e.g. cardiovascular disorders, cancers, diabetes. At the same time, gene therapy is a promising tool for angiogenesis manipulation. Its wide clinical application is, however, limited by the lack of suitable theranostic gene delivery systems. To address this issue, in this study we intended to develop an efficient method for magnetically guided manipulation of endothelial cells. First, we defined optimal conditions for HUVEC transfection with microRNA (miR) using polyethyleneimine-magnetic nanoparticle based vector (PEI/MNP), pre-designed in our group. We found that miRNA can be efficiently delivered with PEI/MNP vector without affecting cell survival and functionality. Furthermore, the presence of magnetic component offers certain targeting and non-invasive MRI monitoring opportunities, which we investigated in vitro. Two different approaches were assessed: direct vector guidance and magnetic targeting of transfected cells. As a result, production of miR/PEI/MNP-modified magnetically responsive cells has been selected for further investigation as it was more efficient. We have demonstrated in vitro that up to 80% of transfected cells can be driven to the site of interest by magnetic field. In addition, properties of engineered cells were monitored up to 96 hours and proposed modification strategy has been proven safe. Further, transfected cells were tested in vitro in terms of MRI detection potential. We observed that 104 cells can be detected, when loaded with at least 0.2 pg iron/cell. This and higher intracellular iron concentrations were achieved at optimal transfection conditions defined previously. Moreover, we showed that miR/PEI/MNP-modified endothelial cells do maintain intercellular gap-junctional communication and potentially can exchange miR, serving thereby as its carrier. Taken together, obtained results are indicating that miR/PEI/MNP-modified cells are particularly promising as a multifunctional tool for magnetically guided angiogenesis regulation.
Abstract 2497: Immune checkpoint inhibitors enhance benefits of modified vaccinia virus Ankara to improve survival in preclinical models of cancer
Authors: Karola Rittner, Christelle Remy-Ziller, Julie Hortelano, Isabelle Farine, Micael de Meyer, Virginie Nourtier, Murielle Gantzer, Christine Thioudellet, Philippe Slos, Xavier Preville
Journal: Cancer Research (2015)

Abstract TG4010 is a Modified Vaccinia virus Ankara (MVA) expressing human interleukin 2 and the human mucin1 (MUC1) tumor associated antigen. TG4010 has demonstrated clinical benefit for advanced non small cell lung cancer patients in combination with standard of care chemotherapy in two phase 2 randomized and controlled clinical trials (NCT00415818 and NCT1383148). Immunotherapy based on the use of immune checkpoint blockers such as anti PD-1 and anti PD-L1 has also demonstrated efficacy in phase 2 trials. Hence, the combination of both approaches appears to be of great interest considering the high unmet medical need of this pathology. We developed experimental primary tumor and lung metastasis models to evaluate the rationale of combining both approaches at the preclinical level. Using two CT26 cell lines expressing different target antigens (β-galactosidase and MUC1) we evaluated the impact on overall survival of combined MVA-based and immune checkpoint-based immunotherapies. Synergistic increase in overall survival was observed in the therapeutic CT26-CL25 primary tumor and lung metastasis models upon treatment of mice with the combination of MVA-βgal and anti CTLA4 or anti PD-1 in comparison with either treatment alone. We provide evidence that TG4010 synergized with immune checkpoint inhibitors to increase overall survival in the therapeutic CT26-MUC1 tumor models in comparison with either treatment administrated independently. These observations were associated with an increase in the frequency and the quality of antigen-specific tumor infiltrating CD8+ T cells. These studies pave the way for the evaluation of combinatorial therapies including TG4010 and immune checkpoint blockers in the clinic. Citation Format: Karola Rittner, Christelle Remy-Ziller, Julie Hortelano, Isabelle Farine, Micael de Meyer, Virginie Nourtier, Murielle Gantzer, Christine Thioudellet, Philippe Slos, Xavier Preville. Immune checkpoint inhibitors enhance benefits of modified vaccinia virus Ankara to improve survival in preclinical models of cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2497. doi:10.1158/1538-7445.AM2015-2497
The Biologic Relevance of PIM Kinases in the Context of Multiple Myeloma and Their Potential As Therapeutic Targets in Combination Drug Therapy
Authors: Janani Ramachandran, Loredana Santo, Homare Eda, Dharminder Chauhan, Ka Tat Siu, Miroslav Koulnis, Dennis Huszar, Kenneth C Anderson, Noopur S. Raje
Journal: Blood (2015)
Abstract 564: The expression of fetal oncogene 5T4 in CTCs obtained from NSCLC patients is discordant with the expression measured in the primary tumor
Authors: Steven R. Pirie-Shepherd, Shibing Deng, Jonathon Golas, Pamela Vizcarra, Eric Tucker, Dena Marrinuci, Hans-Peter Gerber, Eric L. Powell
Journal: Cancer Research (2015)

Abstract The fetal oncogene 5T4 is a cell surface protein, with over-expression observed in a variety of cancers as compared to normal adult tissue. Recent studies have shown that expression of 5T4 appears to be associated with the undifferentiated state and the epithelial-mesenchymal transition (EMT), and thus has been associated with a more invasive phenotype. We have developed assays to measure the expression of the fetal oncogene, 5T4, in both the primary tumor compartment and in the circulating tumor cell compartment. These assays were then used to investigate 5T4 expression in a small cohort of patients with NSCLC. We obtained matched primary tumor and blood samples, with the blood being obtained prior to resection of the primary tumor. The expression of 5T4 was found to be robust and measurable in both the primary and circulating tumor compartments. We observed expression of 5T4 in both adenocarcinoma and squamous cell carcinoma, in all stages and grades of tumor, with no specific correlation between expression and stage, grade or pathology. We further observed robust enumeration of CTCs in NSCLC samples. The expression of 5T4 was heterogeneous in the CTC compartment with no correlation to grade, stage or pathology. Finally, we observed no concordance between 5T4 expression in the primary tumor and the circulating tumor cell compartment. We discuss the current utility of target expression in predicting response to targeted therapy in the context of antibody based therapy, and the role that CTCs may have in the clinic. Citation Format: Steven R. Pirie-Shepherd, Shibing Deng, Jonathon Golas, Pamela Vizcarra, Eric Tucker, Dena Marrinuci, Hans-Peter Gerber, Eric L. Powell. The expression of fetal oncogene 5T4 in CTCs obtained from NSCLC patients is discordant with the expression measured in the primary tumor. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 564. doi:10.1158/1538-7445.AM2015-564
S71 Do children with troublesome preschool wheeze have evidence of fungal sensitisation?
Authors: KA Holden, KG Staley, EA Gaillard
Journal: Thorax (2016)

<h3>Introduction</h3> Preschool wheeze is very common and can be troublesome necessitating frequent hospital admissions and trials of preventer medications. It has been suggested that fungal sensitisation is associated with severe childhood asthma (Vicencio 2014), however it is unclear when this sensitisation occurs and whether fungal sensitisation is associated with preschool wheeze. We aimed to assess whether children attending our clinic due to preschool wheeze were sensitised to fungi and whether fungal sensitisation is associated with troublesome wheezing. <h3>Methods</h3> Preschoolers attending our clinic due to wheezing investigated for allergic sensitisation to a panel of aeroallergens (<i>D. pteronyssinus</i>, cat, dog, timothy grass and tree pollen) and fungi were identified. Evidence of sensitisation was determined by a raised specific IgE (sIgE) &gt; 35 kU/L detected by fluorescent enzyme immunoassay. Sensitisation to fungi was determined either by raised sIgE to a ‘mould screen’ (comprising <i>Penicillium notatum</i>, <i>Cladosporium herbarum</i>, <i>Aspergillus fumigatus</i>, <i>Candida albicans, Alternaria alternata and Helminthosporium halodes</i>) or to specific fungi including those in the mould screen and <i>Malassezia spp</i>. Data on the number of hospital-recorded admissions due to an exacerbation of wheezing within the preceding 12 months and currently prescribed medications, hence BTS treatment step, were collected. <h3>Results</h3> Table 1 displays the demographic features of the 51 children identified, the number of children with a raised total IgE and evidence of sensitisation to aeroallergens and fungi. Six (24%) children with a raised total IgE and 4 (31%) sensitised to aeroallergens were sensitised to fungi. Whilst we found a positive correlation between age and serum total IgE (0.364, r<sup>2</sup> = 0.13, p &lt; 0.01 – Spearman’s rank) there was no significant correlation between age and sIgE to the mould screen or specific fungi. We found no significant differences in the age, number of hospital-recorded admissions due to wheeze or BTS treatment step between those preschoolers sensitised and not-sensitised to fungi. <h3>Discussion</h3> Our preliminary data suggests that a) few children with preschool wheeze attending clinic have evidence of fungal sensitisation and b) fungal sensitisation does not appear to be associated with hospitalisation frequency or required medications. Further exploration is required to determine, if any, the relationship between fungal sensitisation and preschool wheeze.
Subclonal Mutations in SETBP1 Predict Relapse in Juvenile Myelomonocytic Leukemia
Authors: Elliot Stieglitz, Camille B Troup, Laura C Gelston, Eric D Chow, Kristie B Yu, Jon Akutagawa, Amaro N Taylor-Weiner, Y. Lucy Liu, Peter D Emanuel, Benjamin S Braun, Robert B. Gerbing, Todd A. Alonzo, Mignon L. Loh
Journal: Blood (2014)
Abstract 4403: Antitumor properties of ouabain in lipid double emulsion integrated with tumor cell membrane fractions
Authors: Jun Wu, Mariele Mondala, Meng-Yin Hsieh, Eugene Roberts, Richard Ermel
Journal: Cancer Research (2015)

Abstract Cell membrane proteins such as integrins are crucial for cell-cell recognition, binding and communication, cell-stromal interaction and adhesion, tumor invasion and metastasis. It was reported that cell-size hydrophobic beads coated with tumor membrane molecules could stimulate the lymphokine activated killer (LAK) cells to release IFN-alpha and TNF-alpha, and interrupted the recognition between LAK cells and tumor cells (Chong AS, 1988, 1991). Lipid double emulsion is a convenient formulation method to integrate lipid materials, hydrophilic and hydrophobic drugs in uniformed particles to achieve combinational chemotherapy. However the nonspecific uptake of double emulsion particles in vivo limits its targeting therapeutic potential. In current study, we sought to coat double emulsion particles with tumor cell membrane fraction to mimic the tumor cell surface in order to enhance the interaction and uptake of emulsion particles into tumor cells. Canine osteosarcoma cells and lymphoma cells were chosen for this study because canine cancers develop in immunocompetent environments and share many similar features observed in human cancers, such as long periods of latency for initiation, progression and development, metastasis, acquired drug resistance and recurrence. We use ouabain as therapeutic warhead in the emulsion particles because ouabain is known to have extremely high anticancer potential at nanomolar concentration level from our previous studies. The major obstacle of applying ouabain in vivo therapy is that the therapeutic dose window of ouabain is very narrow. Therefore in an attempt to mitigate such narrow therapeutic margin of safety, ouabain was formulated in lipid double emulsion by using microfluidic synthesis method and membrane emulsification method. The double emulsion particles were further coated with osteosarcoma cell membrane fraction. Our preliminary results showed enhanced antitumor activities of ouabain in tumor cell mimic-double emulsion or nanoemulsion against canine osteosarcoma cells and lymphoma cells in vitro and in orthotopic xenograft models in vivo, suggesting a promising therapeutic benefit in canine cancer targeting therapy. Citation Format: Jun Wu, Mariele Mondala, Meng-Yin Hsieh, Eugene Roberts, Richard Ermel. Antitumor properties of ouabain in lipid double emulsion integrated with tumor cell membrane fractions. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4403. doi:10.1158/1538-7445.AM2015-4403
Cellular Uptake of Soluble Neutrophil Elastase Increases Cyclin E (CCNE) Isoform Expression and Significantly Augments Susceptibility of Breast Cancer Cells to Cytolysis by CCNE-Specific Cytotoxic T Lymphocytes
Authors: Elizabeth Mittendorf, Gheath Alatrash, Na Qiao, Pariya Sukhumalchandra, Sijie Lu, Kathryn Quintanilla, Karen Clise-Dwyer, Jeffrey J. Molldrem
Journal: Blood (2010)
Identification of Defects in the Transcriptional Program during Differentiation of CD34+Cells Selected from Patients with Both, Low- and High-Risk Myelodysplastic Syndromes.
Authors: Martina Komor, Saskia Gueller, Sven de Vos, Oliver G. Ottmann, Dieter Hoelzer, Wolf-Karsten Hofmann
Journal: Blood (2004)
Megaloblastic Anemia and Mitochondriopathy Caused by a Homozygous Mutation in Sideroflexin-4.
Authors: Jeffrey D. Cooney, Gordon J. Hildick-Smith, Caterina Garone, Jonathan N. Thon, Orhan H. Akman, Caiyong Chen, Dhvanit I. Shah, Diana Santos Branco, Non Miyata, Matthew L. Freedman, Sarah E. Calvo, Vamsi K. Mootha, Carla M. Koehler, Joseph E. Italiano, M. Alice Donati, Salvatore DiMauro, Barry H. Paw
Journal: Blood (2012)
Interleukin-6 Is a Significant Modifier of the Anemia Associated with Aging in Mice.
Authors: Bryan J. McCranor, Jacqueline M. Langdon, Olivier D. Prince, Qilu Yu, Alan E. Berger, Chris Cheadle, Curt I. Civin, Jeremy D. Walston, Qian-Li Xue, Cindy N. Roy
Journal: Blood (2012)
Abstract 456: Defined co-mutation subgroups of KRAS-mutated NSCLC display distinct immune profiles
Authors: Warren L. Denning, Lixia Diao, Ferdinandos Skoulidis, Yanyan Lou, Lauren Byers, Jing Wang, John Weinstein, Don Gibbons, John Heymach
Journal: Cancer Research (2015)

Abstract Traditional pathological divisions of lung cancer are giving rise to molecular classifications that identify unique drivers of tumor malignancy. Of these molecular classifications, KRAS represents about 30% of all lung adenocarcinomas. Our group has recently investigated if co-mutations within KRAS-mutated lung tumors can aid in identifying a unique therapeutic strategy for patient treatment. Given that tumors exert a strong influence over the immune system and the recent successes in immune targeting via checkpoint blockade therapy in various cancers, including lung, we hypothesized that co-mutational subgroups of KRAS-mutant NSCLC elicit distinct immune profiles and support a rationale for evaluating these subgroups in conjunction with appropriate immunotherapies for further clinical testing. To investigate this phenomenon, we performed a supervised analysis of 86 immune-related genes from The Cancer Genome Atlas data set in lung adenocarcinomas with KRAS mutations and TP53 or STK11(LKB1) co-mutations. The results from our analysis revealed that KRAS-mutant NSCLC with LKB1 (KL) and TP53 (KP) co-mutations have distinct immune profiles. KP tumors have an increase in (1) PD-L1 and PD-L2; (2) co-stimulation via CD40, CD80, CD86; (3) antigen presentation HLA-I, HLA-II, and CD1; (4) FOXP3 and CD4, indicating T regulatory populations; (5) CD33, CD11b, and CD11c, which are potentially suppressive myeloid cell populations. We conclude that KRAS co-mutations can create significantly different immune microenvironments; KRAS + TP53 (KP) mutant tumors may specifically benefit from checkpoint blockade, depletion of immunosuppressive myeloid and T regulatory populations, but could support de novo anti-tumor T cell expansion due to high expression of co-stimulatory molecules and HLA-I and HLA-II expression. Conversely, KRAS + LKB1 (KL) mutant tumors have an immunosuppressed or inert phenotype and may potentially benefit from adoptive cell transfer of anti-tumor T cells in conjunction with or in lieu of checkpoint blockade. Citation Format: Warren L. Denning, Lixia Diao, Ferdinandos Skoulidis, Yanyan Lou, Lauren Byers, Jing Wang, John Weinstein, Don Gibbons, John Heymach. Defined co-mutation subgroups of KRAS-mutated NSCLC display distinct immune profiles. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 456. doi:10.1158/1538-7445.AM2015-456
Abstract 3297: Cell phone use and cytokines expression in saliva of the parotid glands
Authors: Fabrício TA de Souza, Carolina C. Gomes, Luiz Armando De Marco, Elisa C. Siqueira, Samuel M. Costa, Jeane F. Correia-Silva, Ricardo S. Gomez
Journal: Cancer Research (2015)

Abstract The association between cell phone use and the development of parotid tumors is controversial. Because there is unequivocal evidence that the microenvironment is important for tumor formation, we investigated in the parotid glands whether cell phone use alters the expression of cytokines. We used the saliva produced by the parotid glands to assess cytokines levels, comparing the saliva from the gland exposed to cell phone radiation (ipsilateral) to the saliva from the opposite, unexposed parotid (contralateral) of each individual. We compared salivary flow, total protein, IL-1 β, IL-6, IFN-γ and TNF-α between the ipsilateral and contralateral parotids of forty-three individuals. No difference was found for any of these parameters. Our study provides evidence that the exposure of parotid glands to cell phone use does not alter cytokines levels in the parotid. Supported by CAPES, FAPEMIG and CNPq, Brazil. Citation Format: Fabrício TA de Souza, Carolina C. Gomes, Luiz Armando De Marco, Elisa C. Siqueira, Samuel M. Costa, Jeane F. Correia-Silva, Ricardo S. Gomez. Cell phone use and cytokines expression in saliva of the parotid glands. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3297. doi:10.1158/1538-7445.AM2015-3297