tbuckley/cabot-search huggingface dataset:
A2.31 Immunisation with type II collagen (CII) alters the IL-23 receptor expression profile compared to naïve conditions
Authors: W Razawy, CH Alves, PS Asmawidjaja, AMC Mus, M Molendijk, W Dankers, M Oukka, VK Kuchroo, E Lubberts
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background and objectives</h3> Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterised by synovial infiltration of pro-inflammatory cells. In RA, the interleukin-23/17A (IL-23/IL-17A) pathway plays a central role. While in human RA increased IL-23 levels correlate with higher disease activity, IL-23deficient mice are protected against collagen induced arthritis. Under naïve conditions, it was shown that IL-23 receptor (IL-23R) positivecells were mainly located in the lymph nodes (LNs), but not in the spleens of IL-23R GFP-reporter mice. While these cells were mainly composed of T cells, macrophages and dendritic cells (DCs), no IL-23R expression was found on CD8<sup>+</sup> T cells, B cells or NK cells.&nbsp;However, since IL-23R expression can be altered in an inflammatory condition, we aimed to analyse expression of IL-23R on immune cells in lymphoid tissues of type II collagen (CII) immunised mice. <h3>Materials and methods</h3> IL-23R GFP-reporter mice and littermate wild type controls were immunised with 100 µg chicken type II collagen in complete Freund's adjuvant. After 10 days, LNs and spleens of mice were harvested and IL-23R expressing cell types were analysed using flow cytometry. In addition, splenocytes of IL-23R GFP-reporter mice were cultured overnight with IL-23, in order to assess their responsiveness. <h3>Results</h3> In contrast to naïve mice, IL-23R expression was detected on B cells and CD8<sup>+</sup> T cells in LNs of CII immunised mice. While T cells were the most prominent IL-23R-expressing cell type in the LNs, DCs formed the main IL-23R<sup>+ </sup>population in the spleens. Furthermore, IL-23R expression was found on splenic B cells. Within the T cell population, γδ T cells form the largest IL-23R<sup>+</sup> cell type in both the LNs and spleens. <i><i>In vitro</i></i>, splenocytes of IL-23R GFP-reporter mice respond to IL-23 by upregulating IL-23R gene expression. <h3>Conclusions</h3> In contrast to naïve mice, IL-23R<sup>+</sup> cells such as DCs, B cells and γδ T cells were detected in the spleens of CII immunised mice. Furthermore, γδ T cells, B cells and CD8<sup>+</sup> T cells express IL-23R in the LNs. These data suggest that immunisation with CII alters the IL-23R expression profile compared to naïve conditions.
Hemorrhagic Cutaneous Anaphylaxis Due to Autosensitization to Deoxyribonucleic Acid
Authors: Robert S. Schwartz, F. Bruce Lewis, William Dameshek
Journal: New England Journal of Medicine (1962)

THE pathogenesis of certain disorders, such as rheumatoid arthritis and systemic lupus erythematosus, has, in recent years, been attributed to autoimmunization. Although a number of antibodies have been detected in the serum and tissues of patients with these diseases the nature of the antigenic stimulus remains poorly defined. Recently, we observed a patient with a peculiar hemorrhagic eruption involving the skin, and apparently of autoimmune origin, in whom the antigen could be characterized with great precision but antibody could not be found, even with the most sensitive methods available. Our observations were stimulated by the report of Levin and Pinkus, . . .
Abstract 602: Ascorbic acid combined with a cytostatic inhibitor of glycolysis synergistically induces apoptosis in non-small lung cancer cells.
Authors: Saleha B. Vuyyuri, Jacob Rinkinen, Erin Worden, Keith R. Davis
Journal: Cancer Research (2013)

Abstract Purpose An exceptional characteristic of many cancer cells is increased glucose uptake and elevated glycolysis with a concomitant reduction in oxidative respiration, also known as the Warburg effect. The increase in basal levels of reactive oxygen species (primarily, H2O2) and their increased dependence on glycolysis for their survival make tumor cells more susceptible than normal cells to treatment with a combination of pro-oxidant agents and chemotherapy. This common feature of tumor cells has been targeted for the development of new cancer therapeutics. Ascorbic acid (AA) has been shown to have cancer curative potential, at clinically achievable doses that have minimal or no toxic effects on normal cells. We have previously shown that some cancer cell lines are significantly more sensitive to treatment with ascorbic acid at concentrations where it may function as a pro-oxidant. In the present case, we investigated the effects of AA alone or in combinations of AA with the glycolysis inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3-PO) on the viability of three non-small lung cancer (NSCLC) cell lines vs immortalized lung epithelial cell line. Results Cell viability assays showed that combined treatment of AA and 3-PO caused a synergistic increase in cell death in all the NSCLC cell lines at concentrations well below the IC50 concentrations for each compound alone, while no synergistic cytotoxic effect was observed in immortalized lung epithelial cells. Key markers such as DNA fragmentation, PARP cleavage, annexin-V binding, and caspase activity indicated that AA-induced cell death is caused via the activation of apoptosis. Metabolomic analysis revealed a decrease in NAD/NADH in the combination treatment that correlated well with the synergistic cell death observed in the viability results. The importance of NAD/NADH depletion in the synergistic response was confirmed by rescue experiments showing addition of NAD to the culture protected cells from the combination treatment. The major significance of these studies is that, we show for the first time that AA selectively synergizes with a glycolytic inhibitor in killing NSCLC cells and that pharmacologic AA treatment (either alone or combined with any other chemotherapeutic agent or glycolytic inhibitors used in the treatment of cancer) may be a promising therapy for non-small lung cancer and other challenging to-treat cancers. Citation Format: Saleha B. Vuyyuri, Jacob Rinkinen, Erin Worden, Keith R. Davis. Ascorbic acid combined with a cytostatic inhibitor of glycolysis synergistically induces apoptosis in non-small lung cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 602. doi:10.1158/1538-7445.AM2013-602
AB0728 Foot Functional Impact in A Spondyloarthritis Cohort
Authors: T. Meirinhos, R. Aguiar, C. Ambrόsio, A. Barcelos
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> The foot is one of the most complex components of musculoskeletal system, and also one of the less addressed in literature. However, its importance in daily activities (gait, posture, etc) has led to a growing concern in understanding the functional mechanisms and joint kinetics of the foot. Foot involvement, whether articular or enthesopatic, is well known in axial spondyloarthritis (aSpa) and peripheral spondyloartrhritis (pSpa). However, data on literature are scarce, and the true impact in patients is unknown. <h3>Objectives</h3> To determine the prevalence of functional impairment of the foot and tibiotarsal joint in a cohort of patients with aSpa and pSpa and its relation with disease duration, activity and function indexes. <h3>Methods</h3> Cross sectional study. Analyzed variables: gender, age and disease duration. Every patient answered the FAOS (Foot and Ankle Outcome Score) questionnaire, which assesses pain, functional aspects of the foot and tibiotarsal joint and its impact on quality of life and daily activities. In patients with aSpa, BASDAI and BASFI were obtained, as well as DAS28 in patients with pSpa. Control group: healthy individuals, adjusted by gender and age to patients. Statistical analysis with SPSS® version 18.0 <h3>Results</h3> Eighty patients were enrolled (38 with aSpa and 42 with pSpa), 50 males and 30 females; mean age was 47.3±14.24 years old and mean disease duration was 8.96±10.02 years. Mean DAS28 was 2.49±1.08, mean BASFI was 3.67±2.55 and mean BASDAI was 4.35±2.32. Mean FAOS was 85.5±11.1 on aSpa (mild symptoms) and 77.6±16.1 on pSpa (mild to moderate symptoms). Compared to control group, pSpa patients had lower FAOS values (p&lt;0.03); this difference wasn9t statistically significant with aSpa patients. Although lower FAOS values were found in females and older patients, this difference wasn9t statistically significant. A statistically significant negative correlation was found between FAOS values and disease duration in pSpa patients (p&lt;0.03). A statistically significant negative correlation was also found between disease activity and FAOS values (p&lt;0.04 in aSpa and p&lt;0.02, in pSpa); BASFI values also correlated negatively with FAOS (p&lt;0.04). <h3>Conclusions</h3> In our cohort, most of patients assumed the presence of mild to moderate functional impairment, especially those with pSpa, as expected. Foot involvement seems to relate directly with disease activity, in both aSpa and pSpa, and with disease duration in pSpa. <h3>References</h3> Domingues F., Esteves J., Pereira JP Adaptation and validation of the Foot and Ankle Outcome Score (FAOS) for use in Portugal; Revista Portuguesa de Fisioterapia no Desporto 2008; 1(2):23-32 Sawacha Z et al. Biomechanical assessment of balance and posture in subjects with ankylosing spondylitis; J Neuroengineering Rehabil. 2012;9:63 <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3875
Abstract 5359: Gene expression characterization of different cancer stem cell isolation methods in head and neck cancer
Authors: Bryan Thibodeau, Timothy Geddes, Sandra Galoforo, Jan Akervall, George Wilson
Journal: Cancer Research (2012)

Abstract Background: Head and neck squamous cell cancer (HNSCC) is a heterogeneous cancer that continues to pose major clinical problems. There is growing evidence that cancer stem cells (CSCs) may be an important factor in disease progression and resistance to current treatments. Establishing that stem cells are present in HNSCC and characterizing their global gene expression patterns provides the rationale for this study. Methods: A series of 5 HNSCC cell lines that have been maintained at low passage number such that they maintain phenotypic and morphological characteristics similar to the primary tumor were used in this study. Three different strategies were used to isolate stem cells by flow cytometry sorting: 1) Hoechst 33342 “side” population, 2) Aldefluor, and 3) CD44 bright cells. Global gene expression for each isolated CSC-enriched population and its associated CSC-depleted population was analyzed using Affymetrix Human Exon 1.0ST arrays. Gene set enrichment analysis was performed using Ariadne Pathway Studio. Results: The different methods of identifying CSCs produced widely different populations. In side population cells, there were 77 differentially expressed genes (p&amp;lt;0.05 and 1.5-fold cutoff) while only 59 and 17 genes were differentially expressed in the CD44 bright and Aldefluor bright populations, respectively. Not a single gene was commonly altered between any two of the isolation methods. Gene set enrichment analysis, however, was able to identify categories that differed between the CSC-enriched and CSC-depleted populations. Biological processes of cell cycle, cell division, mitosis, translational elongation, and spindle assembly were highly regulated. Microtubule binding/microtubule plus-end binding and structural constituent of ribosome were commonly altered molecular functions. Conclusions: This study suggests that current methods for isolating putative stem cells in HNSCC result in diverse populations with differing expression of specific genes. However, when examined for global expression patterns, several important categories of genes are commonly altered between the CSC-enriched and CSC-depleted populations. Some of the known characteristics of stem cells such as asymmetric division and unlimited growth potential are suggested by the highly regulated categories of cell division and spindle assembly. From these studies we hope to gain greater insight into the importance of stem cells in HNSCC as well as improve the capability of identifying these potentially important new targets for treatment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5359. doi:1538-7445.AM2012-5359
Management of Fever in Patients with Cancer and Treatment-Induced Neutropenia
Authors: Alastair J.J. Wood, Philip A. Pizzo
Journal: New England Journal of Medicine (1993)

The importance of neutropenia in the risk of serious infection in patients with cancer who are receiving cytotoxic chemotherapy was first recognized nearly 30 years ago1. Since then, considerable progress has been made in the management of fever and neutropenia in both adults and children, resulting in improved survival rates not only for these patients but also for other immunosuppressed patients, including those undergoing organ or bone marrow transplantation and those with bone marrow failure or human immunodeficiency virus (HIV) infection2,3.Some of the current principles for the management of fever in patients with neutropenia are outlined . . .
AB0605 The changes in serum chemokines following leflunomide therapy in patients with rheumatoid arthritis
Authors: P.A. Klimiuk, S. Sierakowski, J. Kita, J. Chwiecko
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> <i>Chemokines play an important role in the pathogenesis of rheumatoid arthritis (RA).</i> <h3>Objectives</h3> We undertook this study to evaluate the effects of leflunomide, an oral pyrimidine synthesis inhibitor, on the serum chemokines levels in patients with active rheumatoid arthritis, who were refractory to treatment with MTX or did not tolerated MTX treatment. <h3>Methods</h3> RA patients were supposed to receive leflunomide (100mg/day loading dose for 3 days followed by 20 mg/day orally for the 12 months). Serum concentrations of RANTES (regulated upon activation, normal T cell expressed and secreted), monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) were assessed by ELISA before and after 3, 6, 9 and 12 months of treatment with leflunomide. <h3>Results</h3> Three months therapy with leflunomide caused reduction in serum RANTES and MCP-1 (in both cases p&lt;0.001) levels. Decrease in the concentration of these chemokines persisted till the end of the study period, but was less significant. In the case of IL-8 its serum levels significantly diminished after 6 months of therapy with leflunomide (p&lt;0.01), and remained stable to the end of the study. Changes in serum chemokine levels were accompanied by significant decrease of disease activity score (DAS) (p&lt;0.001). Prior to the first dose of leflunomide serum concentrations of studied chemokines correlated with marker of RA activity such as the erythrocyte sedimentation rate (ESR) and IL-8 level with disease activity score (DAS). Furthermore, we demonstrated significant correlations between serum levels of RANTES, MCP-1 and IL-8. During study period such associations were far less or not significant. <h3>Conclusions</h3> Leflunomide, beside a clinical improvement, reduce serum chemokines concentrations in RA patients. Leflunomide seems to be an effective treatment for rheumatoid arthritis, alternative to current therapies. <h3>Disclosure of Interest</h3> None Declared
P4-11-15: Increased Propability of Triple Negative Breast Cancer (TNBC) in Premenopausal Patients after Exogenous Hormonal Intake (EHI).
Authors: NF Pistamaltzian, C Tzavara, C Papadimitriou, R Gyftaki, D Tryfonopoulos, C Panopoulos, N Tsoukalas, G Koumakis, S Demiri, N Koufopoulos, Y Misitzis, N Apostolikas, A Efremidis
Journal: Cancer Research (2011)

Abstract Purpose: HRT is a known risk factor for breast cancer (BC) among post-menopausal women. Our knowledge in relation to EHI (estrogens and/or progestin) and BC among pre-menopausal women is scarce. We have studied whether previous EHI influences BC phenotype, clinical and pathologic characteristics and correlation with other known BC risk factors. Patients and methods: A prospective analysis (data recorded upon patients first presentation) of an electronic database at a tertiary cancer centre was performed. Patients’ demographics, risk factors for BC (smoking, alcohol use, obesity, family history), clinical profile, EHI parameters (duration, cause) and the tumors’ histopathology (type, grade,ER/PgR and HER2 by IHC and FISH) were analyzed. Premenopausal patients without an EHI history consisted control group. Results: Out of 938 patients treated for BC between 2006 and 2010, 333(35,5%) were premenopausal and 131(39%) of them reported any use of hormones. Median age was identical (43 years,range:20–57) among premenopausal patients with and without EHI history. Mean duration of use was 28 months (range:1–180). Causes of EHI were contraception (35%), pregnancy(17%), menstrual abnormalities(17%) and medically assisted fertilization(17%). Smoking, alcohol use and obesity didn't differ among two groups. Family history for BC was more common (31, 3% vs. 22,8%, p=0,08)among women with EHI. No correlation was found among duration of use and the time of cancer diagnosis, while the mean time from the cessation of hormones to cancer diagnosis was 13 years(range:1–32). Only 18% of breast cancers were diagnosed within the first 5 years after exogenous hormones cessation. TNBC was found to be significantly increased among premenopausal women with a history for EHI (23,6% vs. 13,4%, p=0,016). This increase was independent of the existence of positive family history for BC (p=0,61). EHI conferred a twofold increase in the risk for a TNBC (OR=1,99 p=0,019). No other clinical or histopathologic parameter showed any difference among the two groups. Conclusion: Prior use of exogenous hormones, for any cause and irrespective of the coexistence of other risk factors and family history, increases the probability of a triple negative breast cancer diagnosis by twofold. Whether this represents a trend of a changing epidemiology in the types of BC in prior hormone users vs. nonusers, poses an extremely challenging hypothesis to be verified in large epidemiologic studies — given the young age and the treating difficulties of this patient population. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P4-11-15.
The Dortmund endoscopic full-thickness resection method: combination of the over-the-scope clip system and the Inoue cap
Authors: Martin Fähndrich, Michael Heike, Marcel Sandmann
Journal: Endoscopy (2014)

The over-the-scope clip (OTSC) system was developed for the closure of perforations and fistulas, and for the endoscopic therapy of gastrointestinal bleeding. Small mucosal and submucosal neoplastic lesions can be aspirated into the cap and, after releasing the clip, a "pseudopolyp" is created. This pseudopolyp located above the closed clip can then be resected using a polypectomy snare. However, placing a snare on the pseudopolyp may be difficult due to the shape of the clip. Herein, we present a dual technique using both the OTSC and the Inoue cap to accomplish endoscopic full-thickness resection (eFTR).
Sclerosing Mediastinitis: Findings on Fluorine-18 Fluorodeoxyglucose Positron Emission Tomography
Authors: M B Imran, K Kubota, S Yoshioka, S Yamada, T Sato, H Fukuda, T Yoshioka, R Kanamaru, T Fujiwara, M Itoh
Journal: Clinical Nuclear Medicine (1999)

Whole-body serial positron emission tomography scanning was done using fluorine-18 fluorodeoxygluclose (F-18 FDG) in a patient with a mediastinal mass. Uptake correlated well with the clinical symptoms and aggressiveness of the disease. Based on the F-18 FDG findings, a biopsy specimen was taken from the active region of the mass, which confirmed the diagnosis of sclerosing mediastinitis.
OUTCOMES OF PERMANENT PACEMAKER IMPLANTATION FOLLOWING TRANSCATHETER AORTIC VALVE REPLACEMENT: ANALYSIS OF THE STS/ACC TVT REGISTRYTM
Authors: Opeyemi Fadahunsi, Abiola Olowoyeye, Anene Ukaigwe, Zhuokai Li, Amit Vora, Sreekanth Vemulapalli, Eric Elgin, Anthony Donato
Journal: Journal of the American College of Cardiology (2016)
HEMODYNAMICS DURING MULTISTAGE SUPINE BICYCLE EXERCISE IN NORMAL SUBJECTS: ASSESSMENT WITH RADIONUCLIDE VENTRICULOGRAPHY AND OBSERVATIONS ON REPRODUCIBILITY.
Authors: Udho Thadani, Michael F. Wilson, Robert D. Burow, Kim Sung, Linda Hardy, Dwight W. Reynolds, Donald Kellerman;
Journal: Clinical Nuclear Medicine (1983)

Thadani, Udho; Wilson, Michael F.; Burow, Robert D.; Sung, Kim; Hardy, Linda; Reynolds, Dwight W.; Kellerman;, Donald Author Information
Availability of Tetanus Immune Globulin
Authors: R. K. Cutter
Journal: JAMA (1967)

<h3>To the Editor:—</h3> Levine et al (<b>200</b>: 341, 1967) give the impression that tetanus immune globulin (human) remains in short supply. It is indeed true that this product was in short supply until last fall but now full stocks are available in all wholesale drug houses and all prescription and hospital pharmacies. By January 1967 our inventory position was sufficiently long to permit our International Division to solicit foreign orders for bulk tetanus immune globulin (human). With the present state of knwoledge in regard to tetanus hyperimmunization, plasmapheresis, and plasma procurement and plasma fractionation, we could, without undue pressure, supply the United States and Canada with prophylactic doses of tetanus immune globulin (human) equal to the total doses of equine and bovine tetanus antitoxin previously used.
Quality in gastrointestinal endoscopy
Authors: Matthew Rutter, Colin Rees
Journal: Endoscopy (2014)

Endoscopy is a powerful and pivotal tool in the diagnosis and management of gastrointestinal pathology. Ensuring that endoscopy is of high quality is fundamental to providing the best care and experience for our patients. High quality endoscopy delivers better health outcomes, better patient experience, and fewer repeat procedures.
P1168 ITPA AND SLC29A1 GENOTYPING BEFORE TRIPLE THERAPY PREDICT SEVERITY OF RIBAVIRIN-INDUCED ANEMIA
Authors: L. Milazzo, S. Landonio, C. Magni, C. Gervasoni, E. Calvi, S. Cheli, E. Clementi, M. Galli, F.S. Falvella
Journal: Journal of Hepatology (2014)
P143 ACTIVATED MONOCYTES EXPRESS IFN-λ RECEPTOR 1 AND EFFECTIVELY TRIGGER IFN-γ SECRETION OF NK CELLS AFTER STIMULATION WITH IFN-λ
Authors: B. Krämer, B.S. Sastre Turrión, A. Glässner, M. Eisenhardt, F. Wolter, P. Kokordelis, H.D. Nischalke, C.P. Strassburg, U. Spengler, J. Nattermann
Journal: Journal of Hepatology (2014)