tbuckley/cabot-search huggingface dataset:
A1.16 Role of IL-8 and its receptor in anti-citrullinated protein antibody mediated osteoclastogenesis in ra
Authors: Y Liu, A Krishnamurthy, AH Hensvold, V Joshua, H Wähämaa, M Sun, M Engstrom, V Malmström, B Rethi, LA Jopling, AI Catrina
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background and objectives</h3> Circulating levels of IL-8 is related to the bone loss associated with breast cancer metastasis. Recently, we have shown that anti-citrullinated protein antibodies (ACPA) can induce&nbsp;osteoclastogenesis. We aimed to investigate the role of IL-8 and its receptors in mediating osteoclastogenesis in presence or absence of ACPAs <h3>Methods</h3> IL-8 levels was measured by ELISA in serum of risk RA patients and synovial fluid samples of RA patients. CD14 positive monocytes were used to generate osteoclasts with M-CSF and RANKL. Inhibition of IL-8 and its receptor were tested with IL-8 neutralising antibody or small molecule CXCR1/2 anta-agonist (Reparixin and SCH-527123) on osteoclasts (OC) in the presence or absence of ACPA. The number of the OC's were&nbsp;counted in light microscope after TRAP staining. Cell counting kit 8 (CCK8) assay was performed to detect cytotoxicity. CXCR1 and CXCR2 expression was analysed using flow cytometry and immunohistochemistry during different days of osteoclasts maturation. <h3>Results</h3> Increased IL-8 levels were observed in the serum of ACPA positive at risk RA individuals and in synovial fluid of established ACPA positive RA patients. Exogenous IL-8 dose dependently increased OC numbers in presence of RANKL. IL-8 neutralising antibody inhibited the OC numbers even in the presence of ACPA. Reparixin and SCH-527123 significantly inhibited RANKL mediated osteoclastogenesis at 100 µM concentration. No cytotoxicity of the drugs were detected with CCK8 assay. With flow cytometry, we observed CXCR1 and &nbsp;CXCR2 expression in the OC precursors at day 0 and in presence of RANKL the expression was completely lost at day 1 and it reappeared at day 4. Immunohistochemistry staining confirmed the CXCR1/2 expression on OC precursors. <h3>Conclusions</h3> Our data provides insights in to the importance of IL-8 autocrine loop in RANKL and ACPA mediated OC development. IL-8 receptor blockade might be a novel way of targeting osteoclasts in RA.
CHALLENGES IN ACCESS: SOCIOECONOMIC AND DEMOGRAPHIC DISPARITIES IN PATIENTS RECEIVING TRANSCATHETER AORTIC VALVE REPLACEMENT FOR SEVERE AORTIC STENOSIS
Authors: Anna Sleder, Rashad Farha, Iyad Isseh, Oleksandra Lupak, Shiloh Tackett, Kimberly Baker-Genaw, William O’Neill
Journal: Journal of the American College of Cardiology (2015)
Book: Intensive Care Medicine
Authors: I. Barker
Journal: BMJ (1999)

Ed Julian F Bion BMJ Books, £45, pp 456 ISBN 0 7279 1076 0 Rating: ![Graphic][1]</img>![Graphic][2]</img>![Graphic][3]</img> Anaesthesia has its roots in the basic sciences and interfaces with every branch of medical practice. The specialty thus seems particularly suited to the publication of series of books that are more than journals but, individually, are not comprehensive textbooks. Intensive Care Medicine is the latest in the BMJ's series Anaesthesia and Acute Medicine . The editor is a recognised authority in the specialty, the book consists of 32 monographs by 52 authors, mostly from Britain but including some from continental Europe, Australia, and north America. It is refreshing that some of the contributors are trainees; the book cannot be regarded as merely the idiosyncratic … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif
THE HEMODYNAMIC EFFECTS OF INTRAVENOUS INOTROPE/VASODILATOR THERAPY IN THE BETA-BLOCKER ERA
Authors: Sara Kalantari, Jonathan Grinstein, Bryan Smith, Gabriel Sayer, Sirtaz Adatya, Nitasha Sarswat, Gene Kim, Daniel Burkhoff, Nir Uriel
Journal: Journal of the American College of Cardiology (2016)
Evaluation of Dementia
Authors: D S Geldmacher, P J Whitehouse
Journal: New England Journal of Medicine (1996)

Dementia is the clinical syndrome characterized by acquired losses of cognitive and emotional abilities severe enough to interfere with daily functioning and the quality of life. The term does not imply a specific cause or pathologic process. More than 55 illnesses, some nonprogressive, can cause dementia.1 It occurs primarily late in life; the prevalence is about 1 percent at the age of 60 years and doubles every five years, to reach 30 to 50 percent by the age of 85.2,3 Because all types of dementia are treatable, at least with psychosocial interventions, accurate diagnosis is essential to determine the . . .
IMPACT ON DELAYS AND OUTCOMES OF A STRATEGY OF INITIAL ECG-GUIDED CULPRIT VESSEL INTERVENTION COMPARED TO CONTRALATERAL OR COMPLETE DIAGNOSTIC ANGIOGRAPHY PRIOR TO INTERVENTION IN ST-SEGMENT ELEVATION MYOCARDIAL INFARCTION
Authors: Etienne Couture, Michel Nguyen, Simon Berube, Karl Dalery, André Gervais, Richard Harvey, Benoit Daneault
Journal: Journal of the American College of Cardiology (2015)
Abstract P4-09-08: PTEN Protein Expression in Postmenopausal Steroid Receptor Positive Early Breast Cancer Patients Treated with Adjuvant Tamoxifen
Authors: ZM Milovanovic, SV Susnjar, VS Plesinac-Karapandzic, ZD Juranic, SB Tatic, RR. Dzodic
Journal: Cancer Research (2010)

Abstract Background: Since one of possible causes of resistance to antiestrogen therapy in SR+BC patients is an alteration of PTEN (Phosphatase and Tensin homolog deleted on chromosome 10) signaling pathways, the aim of this study was to determine the PTEN protein expression in postmenopaual patients with steroid receptor (SR)+ breast cancer (BC) treated with adjuvant tamoxifen, to investigate the association of PTEN protein expression with tumor histology, size and grade, estrogen receptor (ER), progesteron receptor (PR) and human epidermal growth receptor 2 (HER2) statuses and disease outcome, as well. Patients and methods: This was a retrospective analysis of 78 postmenopausal SR+BC patients treated for stage I/II with adjuvant tamoxifen. PTEN protein expression and ER, PR and HER2 status were determined using immunohistochemistry method. Results: The distribution of PTEN protein expression according to tumor histology was as follows: PTEN+ status in 27/43 (62,8%) patients with ductal and in 26/35 (74,3%) patients with lobular carcinomas and PTEN-status in 16/43 (37,2%) patients with ductal and in 9/35 (25,7%) patients with lobular carcinomas. Disease relapse was observed in 38/78 pateints: 14/53 (26.4%) of PTEN+BC subgroup and 24/25 (96%) of PTEN-BC subgroup. There were no significant association between PTEN protein expression and tumor histology, size and grade, and ER, PR and HER2 expression, as well. Patients with PTEN-BC had significantly shorter disease-free interval and overall survival (for both Log rank test, P&amp;lt;0.01) compared to PTEN+BC pateints. Conclusion: Our results suggest that PTEN protein expression might be of prognostic significance in postmenopausal SR+ BC patients treated with ajduvant Tamoxifen. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P4-09-08.
Broad‐Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells
Authors: Chuan Yang, Sangeetha Krishnamurthy, Jie Liu, Shaoqiong Liu, Xiaohua Lu, Daniel J. Coady, Wei Cheng, Gennaro De Libero, Amit Singhal, James L. Hedrick, Yi Yan Yang
Journal: Advanced Healthcare Materials (2016)

In this study, a series of star‐shaped polycarbonates are synthesized by metal‐free organocatalytic ring‐opening polymerization of benzyl chloride (BnCl) and mannose‐functionalized cyclic carbonate monomers (MTC‐BnCl and MTC‐ipman) with heptakis‐(2,3‐di‐O‐acetyl)‐β‐cyclodextrin (DA‐β‐CD) as macroinitiator. The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution. After deprotection of ipman groups and quaternization with various N,N ‐dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis. Furthermore, the optimal mannose‐functionalized polymer demonstrates mannose receptor‐mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose. Taken together, the materials designed in this study have potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria.
RELACIÓN ENTRE LA POSICIÓN HORARIA DE LA VÁLVULA ILEOCECAL Y LA FACILIDAD EN SU INTUBACIÓN
Authors: B Velayos, G González, L Del Olmo, MF Muñoz, L Fernández, N Alcaide, R Aller, L Ruiz, A Macho, S Gómez, JM González
Journal: Endoscopy (2014)

Objetivo: Estudiar si la posición horaria de la válvula ileocecal (VIC) condiciona una intubación ileal rápida.
AB0669 The importance of diagnostic ultrasound in the differential diagnosis of painful shoulder
Authors: J. Jovanovic, V. Skakic, V. Jovanovic, S. Bozilov, A. Skakic
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> The aim of this study was to evaluate the diagnostic significance of musculoskeletal ultrasound (MSUS) in determining the type and presence of soft tissue changes in patients with a referral diagnosis of painful shoulder <h3>Methods</h3> We examined 63 patients or 67 shoulders, who had referral diagnosis of painful shoulder. Patients with trauma for the past year and what suffering from connective diseases were not analyzed. MSUS examination was performed using 8-13 MHz linear probe. <h3>Results</h3> Of the 63 patients there were 34 (54%) and 29 women (46% men), mean age 52.4 years (range 21 to 69 years) who were divided into 3 groups according to age. Of 63 patients 48 (76.2%) patients had abnormal and 15 (23.8%) patients had normal results. There were a total of 76 changes and the most common soft tissue pathology is chronic supraspinatus calcific tendinitis 42.1%. The highest percentage of pathological findings were found in the patients of 41-60 years and the lowest percentage in the group of 21-40 years. In the oldest group, the findings of a complete rupture of the rotator cuff. The most common changes detected, at the supraspinatus tendon 64.5% as follows: Chronic calcific tendinitis in 32 (42.1%), complete rupture of the rotator cuff in 10 (13.2%) and partial rupture of the rotator cuff in 7 (9.2%) patients. Subdeltoid bursitis was found in 7 (9.2%), long head biceps tenosynovitis in 9 (11.9%) patients. Subscapularis tendinitis was found in 4 (5.3%) and infraspinatus tendinitis in 1 (1.3%). Joint effusion in humeroscapular in 3 (3.9%) and tendinitis m.bicepsa brachi in 3 (3.9%) patients. <h3>Conclusions</h3> Ultrasound has an important place in the diagnosis of painful shoulder. As a non-invasive, relatively inexpensive and available methods, and because of the high specificity in detecting soft tissue changes the method of choice in the diagnosis of painful shoulder <h3>Disclosure of Interest</h3> None Declared
Balloon-catheter-assisted endoscopic snare resection for choledochocele using a single-channel duodenoscope
Authors: J. Hiratsuka, T. Akiba, M. Kucera Kesties, P. Brant, L. Costa Almeida
Journal: Endoscopy (2012)

Choledochocele, or type III choledochal cyst in Todani's classification, is a rare congenital disease [1] [2] [3]. Pancreatobiliary symptoms and the risk of malignancy are the reasons for treatment, which is usually done by surgical excision or, in some cases, by endoscopic resection [2] [3] [4] [5].
P216 Analysing the success rates of pleurodesis in patients admitted to an elective short stay ward
Authors: AB Bibi, SW Wilson, NA Anwar
Journal: Thorax (2013)

<h3>Introduction</h3> Pleural effusions are a common respiratory problem and account for 20,000 hospital episodes annually. 70% of patients need admission of 7 days or more (1). Treatment options include pleurodesis and long term indwelling pleural catheters (IPC)(2). Traditionally, management has involved recurrent lengthy hospital admissions which are both inconvenient for the patient and expensive. To reduce these problems, we admit patients to an elective short stay ward where they undergo pleurodesis and are discharged after 1–2 days. <h3>Aim</h3> To assess the effectiveness of pleurodesis in patients admitted to an elective short stay ward. <h3>Method</h3> We retrospectively reviewed 33 patients who were managed on an elective ward. A 12F Chest drain was inserted followed by administration of 4g sterile talc. Patients who did not re-attend with an effusion within in the following six months, or prior to dying were considered a success. <h3>Results</h3> During 2009–2010, 33 patients with an average age of 66.5 years underwent pleurodesis on the elective short stay ward. 23/33 patients did not re-attend with an effusion within six months (70%). However, 10 re-attended with a recurrent effusion despite talc (30%). Of these, 3 patients had trapped lung on their xray and 1 had a chylothorax. Both are reported causes of failure (2).Chest drains stayed in for 1–2 days, with an average inpatient stay of 2 days. 18/33 patients died within 3 months of admission (54%). Of the 10 that re-attended, 6 died within 3 months (60%). There were no complications resulting from this procedure. <h3>Conclusions</h3> Patients who are admitted to an elective short stay ward are managed safely and effectively. They require a shorter inpatient hospital stay which is cost-effective. Our results illustrate 70% of patients did not require an IPC but treatment with pleurodesis alone was sufficient to prevent re-attendances.Most patients do not require an acute hospital admission. Patients who do re-attend with a recurrent effusion due to trapped lung can be considered for IPC. <h3>References</h3> Hospital statistics for pleural effusion. DoH.2002–2003. (Accessed June 2013) www.rightdiagnosis.com/p/pleural_effusion/stats.htm. Roberts ME, Neville E et.al. BTS Pleural Disease Guideline. Management of a malignant pleural effusion: <i>Thorax</i>;2010;65(2)
Acute Infectious Lymphocytosis
Authors: Harris D. Riley
Journal: New England Journal of Medicine (1953)

INFECTIOUS lymphocytosis, originally thought to be a variant of infectious mononucleosis, was first described and named by Smith1 in 1941. Its chief characteristic is a benign, relative increase in the number of the small mature lymphocytes in the peripheral blood and in the bone marrow. Original reports listed few or no clinical manifestations, these observations being based on cases from institutions where marked lymphocytosis was considered to be the only unusual characteristic. More recently, however, a wide variety of clinical patterns have been reported in this disease. Smith believes that the disease has a specific infectious basis; he has reported . . .
Book ReviewAmerican Medical Bibliography, 1639–1783: A chronological catalogue, and critical and bibliographical study of books, pamphlets, broadsides, and articles in periodical publications relating to the medical sciences — medicine, surgery, pharmacy, dentistry, and veterinary medicine — printed in the present territory of the United States of America during British dominion and the Revolutionary War.
Authors: Henry R. Viets
Journal: New England Journal of Medicine (1963)
Abstract 1386: Development and application of a syngeneic breast tumor preclinical model to study anticancer efficacy and cardiac safety of doxorubicin and dexrazoxane.
Authors: Yanira Gonzalez-Berrios, Jennifer S. Dickey, Baikuntha P. Aryal, Elliot T. Rosen, Eugene H. Herman, Steven Mog, Asako J. Nakamura, Christophe E. Redon, Ulrich Baxa, Palak Parekh, Karen P. Mason, William Bonner, Ashutosh Rao
Journal: Cancer Research (2013)

Abstract Doxorubicin is a chemotherapeutic drug that causes oxidative stress-mediated cardiotoxicity. To prevent oxidative stress and help to mitigate the cardiotoxic effects of doxorubicin, the iron chelator dexrazoxane is clinically used. However, iron chelators and other antioxidants have not completely succeeded in mitigating this effect and do not protect against iron-independent oxidative damage. One limitation to the development of new cardioprotectants is the lack of physiologically-relevant animal models to simultaneously study antitumor activity and cardioprotection. Therefore, we optimized a syngeneic rat model and examined the mechanisms by which oxidative stress affects the safety and efficacy outcome. Immune-competent spontaneously hypertensive rats (SHRs) were implanted with the SHR-derived, breast tumor cell line, SST-2. Tumor growth and cytokine responses (IL-1A, MCP-1, TNF-α) were observed two weeks post-implantation. In order to demonstrate the utility of SHR/SST-2 model for monitoring both anticancer efficacy and acute cardiotoxicity, we studied cardiotoxic doxorubicin alone and in combination with dexrazoxane. As predicted, significant tumor reduction, DNA damage, and cardiomyopathy were demonstrated by doxorubicin. Treatment with the cardioprotectant dexrazoxane increased the pro-survival autophagy marker LC3-II and decreased caspase-3 in the heart, as a single agent and in combination with doxorubicin. Histopathology and transmission electron microscopy demonstrated apoptosis, autophagy, and necrosis in tumor and heart. Our results demonstrate the advantage of the immune-proficient SHR/SST-2 model to study anticancer agents and simultaneously address anticancer efficacy and cardiac safety. Citation Format: Yanira Gonzalez-Berrios, Jennifer S. Dickey, Baikuntha P. Aryal, Elliot T. Rosen, Eugene H. Herman, Steven Mog, Asako J. Nakamura, Christophe E. Redon, Ulrich Baxa, Palak Parekh, Karen P. Mason, William Bonner, Ashutosh Rao. Development and application of a syngeneic breast tumor preclinical model to study anticancer efficacy and cardiac safety of doxorubicin and dexrazoxane. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1386. doi:10.1158/1538-7445.AM2013-1386
Abstract 1741: Mitotic catastrophe and inhibition of growth of Castration Resistant Prostate Cancers by a dietary agent Withaferin-A.
Authors: Aditi Shirish Vadodkar, Suman Suman, Ram Vinod Roy, Chendil Damodaran
Journal: Cancer Research (2013)

Abstract Cell cycle deregulation has been strongly associated with the pathogenesis of prostate cancer. It has been well documented that an increased expression of cell cycle regulatory proteins (cyclin D1-25%, cyclin A-35% and cyclin B-75%) have been reported in castration resistant prostate cancer (CRPC) cells, when compared to normal prostate tissue. There are several cell cycle regulators undergoing clinical trials targeting either G0/G1 or G2/M phase of cell cycle to inhibit the growth of many cancers including prostate cancer. We previously published that Withaferin-A (WA); a natural compound effectively suppresses CRPC growth both in vitro and in vivo. Hence, in this study we intend to determine the cell-cycle regulatory potential of this agent on CRPC cells. WA caused irreversible G2/M arrest in both CRPC cell lines (PC-3 and C4-2B) till 72 hours . Phosphorylation of cdc2 by Wee1 and Myt1 is an important step to maintain an inactive state of cdc2 that lead to G2/M cell cycle arrest. Additionally decreased levels of phosphorylated Wee-1 and Myt1 followed by increase in the phosphorylation status of cdc2 (Tyr15 and Thr14) supported the G2/M phase of cell cycle arrest in both CRPC cell lines. Further checkpoint kinase assay showed increased activity in WA treated CRPC cells suggesting activation of CDC25C that activates cyclinB/cdc2 complex .We also observed down regulation of several cyclin family proteins in both CRPC cells. Altogether, these results conclude d that WA inhibits CRPC cell growth by induction of G2/M cell cycle arrest, highlighting the potential of WA could be a therapeutic agent for CRPC. Currently, we are investigating the effect of WA on cell cycle markers using both xenograft and TRAMP models. The outcome of our study may enable bringing WA to the mainstream of medicine for prevention and/or therapy for CRPC. Citation Format: Aditi Shirish Vadodkar, Suman Suman, Ram Vinod Roy, Chendil Damodaran. Mitotic catastrophe and inhibition of growth of Castration Resistant Prostate Cancers by a dietary agent Withaferin-A. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1741. doi:10.1158/1538-7445.AM2013-1741
Phase Ii Study of Combined Chemotherapy with 5-Week Cycles of S-1 and Cpt-11 (Iris) Plus Bevacizumab in Patients with Metastatic Colon Cancer
Authors: T. Mizushima, Y. Ide, K. Murata, I. Ohashi, K. Yasumasa, M. Fukunaga, H. Takemoto, H. Tamagawa, T. Kato, M. Ikenaga, J. Hasegawa, T. Hata, I. Takemasa, M. Ikeda, T. Satoh, H. Yamamoto, M. Sekimoto, R. Nezu, Y. Doki, M. Mori
Journal: Annals of Oncology (2014)
P1-07-18: Expanding an Online Tool for Genome-Wide Validation of Survival-Associated Biomarkers in Breast and Ovarian Cancer Using Microarray Data of 3,862 Patients.
Authors: B Gyorffy, A Lanczky, Z Szallasi
Journal: Cancer Research (2011)

Abstract The pre-clinical validation of prognostic gene candidates in large independent patient cohorts is a pre-requisite for the development of robust biomarkers. We earlier implemented an online tool to assess the prognostic or predictive value of the expression levels of all microarray quantified genes in breast cancer patients. In present study, we further expanded our database, added additional analytical options and implemented the tool for ovarian cancer patients. The database was set up using gene expression data and survival information of breast and ovarian cancer patients downloaded from GEO and TCGA (Affymetrix HGU133A, HGU133A 2.0 and HGU133+2 microarrays). After quality control and normalization only probes present on all three Affymetrix platforms were retained (n=22,277). Patients can be stratified into the various robust subtypes either by histology or by various gene expression profiling based methods. To analyze the prognostic value of the selected gene in the various cohorts the patients are divided into two groups according to the median expression of the gene. A Kaplan-Meier survival plot is generated and significance is computed. All together 2,472 breast cancer patients and 1,390 ovarian cancer patients were entered into the database. These groups can be compared using relapse free survival (n=2,414 in breast cancer and 1,090 in ovarian cancer) or overall survival (n=463 and n=1,290). Follow-up threshold has been implemented to exclude long-term effects. The combination of several probe sets can be employed to assess the mean of their expression as a multigene predictor of survival and therapy efficiency. In summary, we expanded our global online biomarker validation platform to mine all available microarray data to assess the prognostic power of 22,277 genes in 2,472 breast and 1,390 ovarian cancer patients. The tool can be accessed online at: www.kmplot.com/breast and www.kmplot.com/dev/ovar. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P1-07-18.