tbuckley/cabot-search huggingface dataset:
Abstract 3746: Association with the sonic hedgehog pathway between gastric cancer stem cells.
Authors: Jae Sook Sung, Jong Won Lee, Ukhyun Jo, Yeul Hong Kim
Journal: Cancer Research (2013)

Abstract Cancer stem cells (CSCs) have been hypothesized to drive the growth, metastasis and chemoresistance capacity. Activation of sonic hedgehog (SHH) signaling has been implicated in progression of variety of tumors. In this study, we investigated potentiality that activation of the SHH pathway maintained the characteristics of gastric CSCs. First, we establish spheres culture system from 5 human gastric cancer cells and identified spheres from 3 gastric CSCs (MKN45, SNU638, SNU601). Compared with parent cells, spheres had more self-renewing capacity and multipotent capacity. Gastric cancer spheres shown that increased expression of stem cell markers (Oct4 and Nanog) and decreased epithelial maker (Cytokeratin 18). However, expressions of CSCs markers (CD44, CD24, CD54 and CD133) were not significantly. Spheres showed more CSCs characteristics, such as more colony formation and more resistance to conventional chemotherapeutic drugs (5-FU, cisplatin and paclitaxel) compared to parent cells. Next, western blot showed that the expression levels of SHH related pathway (Ptch, Smo and Gli1) were significantly higher in spheres than in parent cells. Further analysis, we found that SHH pathway blocked by cyclopamine enhanced the efficacy of chemotherapeutic drugs in spheres. In the further study, we will confirm SHH dependence and in vivo xenograft study. In conclusion, our data suggest that sonic hedgehog blockade could improve the antitumor efficacy of chemotherapy of human gastric cancer. [This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. A010250)] Citation Format: Jae Sook Sung, Jong Won Lee, Ukhyun Jo, Yeul Hong Kim. Association with the sonic hedgehog pathway between gastric cancer stem cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3746. doi:10.1158/1538-7445.AM2013-3746
Mayo Clinic plays $1.26m fine to settle billing error over pathology work
Authors: M. Day
Journal: BMJ (2012)

<h3>Background</h3> Severe bacterial keratitis (BK) typically requires intensive antimicrobial therapy. Empiric therapy is usually with a topical fluoroquinolone or fortified aminoglycoside–cephalosporin combination. Trials to date have not reached any consensus as to which antibiotic regimen most effectively treats BK. <h3>Methods</h3> A systematic review and meta-analysis using Cochrane methodology was undertaken to evaluate the effectiveness of topical antibiotics in the management of BK. Outcomes included treatment success, time to cure, serious complications of infection and adverse effects. <h3>Results</h3> A comprehensive search for trials resulted in 27 956 abstracts for review. This eventually resulted in 16 high quality trials involving 1823 participants included in the review. Treatment success, time to cure and serious complications of infection were comparable among all antibiotic treatments included in the review. Furthermore, there was no evidence of difference in the risk of corneal perforation with any included antibiotics or antibiotic classes. Fluoroquinolones significantly reduced risk of ocular discomfort and chemical conjunctivitis but increased the risk of white precipitate formation compared with aminoglycoside–cephalosporin. Fortified tobramycin–cefazolin was approximately three times more likely to cause ocular discomfort than other topical antibiotics. <h3>Conclusions</h3> Results of this review suggest no evidence of difference in comparative effectiveness between fluoroquinolones and aminoglycoside–cephalosporin treatment options in the management of BK. There were differences in safety profile, however. Fluoroquinolones decreased the risk of ocular discomfort and chemical conjunctivitis while ciprofloxacin increased the risk of white corneal precipitate compared with aminoglycoside–cephalosporin.
SP0205 3 Key Take Home Messages from the Pare Programme
Authors: K. Betteridge
Journal: Annals of the Rheumatic Diseases (2013)

<h3></h3> Each year the EULAR Congress runs a patient stream of sessions co-ordinated by EULAR PARE. The aim of this presentation is to highlight three take home messages from the varied programme offered to patients at the 2013 Congress in Madrid. The presentation will share learning, views and experiences, and highlight the need of people living with RMDs to health professionals. <h3>Disclosure of Interest</h3> None Declared
Variceal hemorrhage of the colon secondary to pancreatic cancer
Authors: Teresa Pinto-Pais, Sónia Fernandes, Luísa Proença, Pedro Roquete, Tiago Pereira, João Carvalho, José Fraga
Journal: Endoscopy (2014)

A 71-year-old woman was admitted due to recurrent lower gastrointestinal bleeding. She had undergone segmental colonic resection and adjuvant chemotherapy for colon adenocarcinoma 10 years earlier, without recurrence on follow-up. She reported experiencing several self-limited episodes of hematochezia over the past 6 months, without other symptoms.
The Third Conference on Hematopoietic Stem Cell Gene Therapy: Biology and Technology
Authors: David W. Emery, David M. Bodine
Journal: Molecular Therapy (2002)

The ability of hematopoietic stem cells (HSC) to repopulate the hematopoietic and possibly other systems, combined with the opportunity to introduce exogenous genes into HSC, provides a unique opportunity for the treatment of hematological and non-hematological diseases. Recently, the first successful application of gene therapy in a clinical setting involved the transfer of the γ-chain of the interleukin (IL)-2 receptor gene (IL2RG) into HSC of patients with X-linked severe combined immunodeficiency-X1 (SCID-X1) [1].
New Approaches Tried in Treating Brain Tumors
Authors: H. McIntosh
Journal: JNCI Journal of the National Cancer Institute (1989)

Journal Article New Approaches Tried in Treating Brain Tumors Get access Hugh McIntosh Hugh McIntosh Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 81, Issue 21, 1 November 1989, Pages 1602–1604, https://doi.org/10.1093/jnci/81.21.1602 Published: 01 November 1989
Modified single-balloon endoscopy for ERCP in a patient with Billroth II gastrectomy
Authors: Chen-Wang Chang, Chien-Yuan Hung, Tai-Cherng Liou, Ching-Wei Chang, Horng-Yuan Wang, Wen-Hsiung Chang, Cheng-Hsin Chu, Ching-Chung Lin, Chih-Jen Chen, Shou-Chuan Shih
Journal: Endoscopy (2014)

An 84-year-old man with a history of hepatocellular carcinoma treated by transcatheter arterial chemoembolization and percutaneous ethanol injection therapy 2 years previously was admitted with jaundice. Magnetic resonance cholangiopancreatography (MRCP) showed a tumor invading the bifurcation of the common bile duct with bilateral intrahepatic duct dilatation ([Fig. 1]). Endoscopic retrograde cholangiopancreatography (ERCP) was considered but as the patient had undergone a previous Billroth II gastrectomy and was concerned about possible complications, we held back from performing this procedure.
EZETIMIBE AND STATIN YIELDS IN SILENT HOLTER AMBULATORY MYOCARDIAL ISCHEMIA (EASY SHAMI)
Authors: Waleed Y. Kadro, Maya Turkmani, Husam Rahim, Osama Elbeshir, Ousama Khateeb, Najeeb Jaha
Journal: Journal of the American College of Cardiology (2016)
ENDOSCOPIA EN PACIENTES ASISTIDOS EN UNIDADES DE ASISTENCIA INMEDIATA CONTROLADAS POR FACULTATIVOS DE MEDICINA INTERNA
Authors: MA Tejedor Cerdeña, MC Piñero Pérez, RD Calderon Begazo, J Umaña Mejía, Y Jamanca Poma, A Fernández Pordomingo, F Geijo Martínez, A Rodríguez Pérez
Journal: Endoscopy (2010)

Introducción: La UCAI (Unidad y Consulta de Atención Inmediata) es una unidad dependiente de Medicina Interna cuyo objetivo es diagnosticar pacientes de forma ambulatoria con la rapidez de un ingresado.
Case 44-1989
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, William F. McNeely, Betty U. McNeely, Mark B. Orringer, Eugene J. Mark
Journal: New England Journal of Medicine (1989)

Presentation of CaseA 74-year-old woman was admitted to the hospital because of severe subxiphoid pain.She was well until four hours earlier, when severe pain developed in the subxiphoid area after she finished breakfast, accompanied by a burning sensation referred to the lower third of the sternum and xiphoid process on swallowing. She was brought to the hospital.The patient was a retired beautician. She underwent a hysterectomy for benign disease 33 years before admission. An earlier diagnosis of multiple sclerosis was made after a temporary right-sided paralysis with diplopia. She had a long history of heartburn, and a . . .
POOR OUTCOME AFTER TRANSCATHETER AORTIC VALVE REPLACEMENT: ASSOCIATIONS OF MORTALITY AND QUALITY OF LIFE WITH FRAILTY IN THE PARTNER TRIAL
Authors: Philip Green, Suzanne Arnold, David Cohen, Ajay Kirtane, Susheel Kodali, David Brown, Charanjit Rihal, Yang Lei, Rebeca Kim, Maria Alu, Martin Leon, Michael Mack
Journal: Journal of the American College of Cardiology (2015)
Abstract P1-15-02: Impact of Retinoblastoma (RB) Tumor Suppressor Pathway on Ductal Carcinoma In Situ (DCIS) Recurrence
Authors: AK Witkiewicz, J Kline, B Freydin, T Apanasovich, G Schwartz, E. Knudsen
Journal: Cancer Research (2010)

Abstract The retinoblastoma (RB) tumor suppressor is functionally inactivated at high frequency in human malignancies including breast cancer. By immunohistochemistry loss of RB function is associated with p16ink4a overexpression and high Ki67 proliferation index.We have previously shown that loss of RB results in dramatic upregulation of genes associated with tumorigenesis and is a key determinant of therapeutic response in invasive breast carcinoma. Recently publish data indicate that RB pathway dysregulation plays an important role in DCIS progression to invasive carcinoma. The aim of this study was to evaluate the expression p16ink4a and Ki67 (indicative of RB loss) in a large cohort of DCIS patients treated at one institution with wide-excision and close follow-up. Material and Methods 149 DCIS patients were included in the study. Expression of p16ink4a and Ki67 was assessed by employing a standard immunoperoxidase method with ani-p16ink4a (MTM Laboratories Westborough, MA) and anti-Ki67 (AbCam Cambridge, MA) antibodies. The p16ink4a staining in DCIS epithelium was scored semi-quantitatively as negative (0; no staining), weak (1;staining in less than 25% of cells), moderate (2; defined as staining in 25-75% of cells) and strong (3; &amp;gt;75% of cells staining). For statistical analysis, the original p16ink4a scores (0, 1, 2, 3) were dichotomized as low (0,1) vs. high (2-3) and original ki67 scores were dichotomized as low (&amp;lt;= 10%) vs. high (&amp;gt; 10%). Stromal p16 staining was scored as absent or present. Association between p16ink4a and Ki67 and nuclear grade, necrosis, hormone receptor and Her2 expression as well as recurrence was assessed using Fisher's exact test. The Cox proportional hazard model was used for analyses of time to recurrence. Results There was a statistically significant association between high p16ink4a and Ki67 expression in DCIS epithelium and triple negative as well as Her2 positive DCIS subtypes. Significant association was detected between high expression of p16 and Ki67 in DCIS epithelium and DCIS recurrence (p=0.005). Stromal expression of p16 was the strongest predictor of DCIS recurrence (p &amp;lt; 0.0001). In multivariate Cox model DCIS cases with high expression of p16 and Ki67 in epithelium and stromal p16 expression had the shortest time to recurrence. Conclusion RB pathway dysregulation in DCIS epithelium as well as stromal expression of p16ink4a indicative of senescence are associated with DCIS recurrence. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P1-15-02.
Reducing the Risks of Relief — The CDC Opioid-Prescribing Guideline
Authors: Thomas R. Frieden, Debra Houry
Journal: New England Journal of Medicine (2016)

The CDC has released a guideline for prescribing opioids for chronic pain to set a safer, more effective course for pain management and opioid use by supporting clinicians caring for patients outside the context of cancer treatment or palliative or end-of-life care.
Abstract 4139: Could dielectrophoretic testing of oral brush biopsies aid early detection and diagnosis of oral cancer?.
Authors: Karen A. Graham, Stefano Fedele, Stephen R. Porter, Nicholas Kalavrezos, James A. McCaul, Mark P. Lewis, Michael P. Hughes, Fatima H. Labeed
Journal: Cancer Research (2013)

Abstract Cancer of the oral cavity and pharynx was the 9th most common cancer in US males in 2009, and has a 5-year survival rate of 50%, which has remained relatively unchanged for several decades. This low survival rate is partly attributed to patients being diagnosed in advanced stages of the disease, and has lead to the consensus that early detection and diagnosis are key to improving survival. The objective of this research was to use a technique called dielectrophoresis (DEP) to study the electrophysiological characteristics of cells from normal, dysplastic and cancerous oral mucosa, collected via oral brush biopsies (OBBs) from patients with oral squamous cell carcinoma (OSCC) or oral epithelial dysplasia (OED), and from healthy volunteers. DEP testing involved applying AC electric fields to the cells collected via brush biopsies, whilst they were suspended in a conductivity-controlled medium. Interactions between the cells and the electric field resulted in cellular movement, dependent both on frequency of the applied electric field and cell properties. The electrophysiological properties of the cells’ cytoplasm and membrane were then deduced as a result of analysing this cellular movement. After obtaining favourable ethical opinions from all institutions involved, OBB samples were collected from 61 patients with OSCC or OED and from 12 healthy volunteers. At the time of abstract submission, 38 OSCC OBB and 12 OED OBB samples (grouped as Abnormal samples, N = 50), 36 patient-matched OBB samples of visually healthy oral mucosa from OSCC and OED patients and 21 OBB samples from the mucosa of healthy volunteers (grouped as Normal samples, N = 57) had been successfully tested and studied using DEP. Statistical analysis of these preliminary results of DEP testing showed statistically significant differences (p&amp;lt;0.025) in cell membrane electrophysiological properties of Abnormal versus Normal OBB samples, OSCC samples versus Healthy Normal samples and OED samples versus Healthy Normal samples. These preliminary results suggest that DEP testing of oral brush biopsies has the potential to form the basis of a minimally invasive bench-top diagnostic tool for rapid identification of cancerous and potentially malignant lesions. Citation Format: Karen A. Graham, Stefano Fedele, Stephen R. Porter, Nicholas Kalavrezos, James A. McCaul, Mark P. Lewis, Michael P. Hughes, Fatima H. Labeed. Could dielectrophoretic testing of oral brush biopsies aid early detection and diagnosis of oral cancer?. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4139. doi:10.1158/1538-7445.AM2013-4139
Nonsegmental Ventilation-Perfusion Scintigraphy Mismatch After Radiation Therapy
Authors: BENNETT B. CHIN, JAMES S. WELSH, LAWRENCE KLEINBERG, DAVID ETTINGER, PETER WHITE
Journal: Clinical Nuclear Medicine (1999)

Purpose: This report illustrates the utility of ventilation-perfusion scintigraphy in differentiating radiation pneumonitis from other causes of dyspnea, including pulmonary embolism, heart failure, obstructive tumor, and chronic obstructive pulmonary disease. Methods and Results: A nonsegmental mismatched perfusion abnormality, which exactly conformed to a radiation port, was diagnostic of radiation pneumonitis. Conclusion: In patients with lung tumors presenting with dyspnea, ventilation-perfusion scintigraphy may be useful in diagnosing radiation pneumonitis and effectively excluding other causes of dyspnea.
FRI0481 Anti- citrullinated protein antibodies but not rheumatoid factor are associated with larger bone erosions in ra patients- a cross-sectional hr-pqct study
Authors: C. F. Hecht, M. Englbrecht, S. Schmidt, J. Rech, K. Engelke, E. Araujo, G. Schett, S. Finzel
Journal: Annals of the Rheumatic Diseases (2013)

Background Anti-citrullinated protein antibodies (ACPA) are known to be associated with joint destruction and a more severe disease course in rheumatoid arthritis (RA) patients. Recently, ACPA have been identified to directly induce bone loss [[1][1]]. Objectives To investigate the influence of ACPAs on periarticular bone structure in RA-patients using high-resolution imaging of bone. Methods 242 RA-patients fulfilling the new ACR/EULAR-criteria received high-resolution peripheral quantitative computed tomography (HR-pQCT) of the 2nd-4th metacarpophalangeal joints of the dominant hand. Age, gender, disease activity (DAS28), disease duration, rheumatoid factor (RF), ACPA (by anti-CCP2 ELISA), and anti-rheumatic therapy were recorded. Number, width, depth and volume of erosions were assessed by 2 independent readers unaware of patients characteristics. For calculation of erosion volumes, a semi-ellipsoid formula was used [[2][2]]. Results 104 patients were RF+/CCP+, 27 RF+/CCP-, 28 RF-/CCP+ and 68 RF-/CCP-. All groups were comparable for age, gender, disease activity, disease duration and anti-rheumatic therapy. 15 patients were excluded due to movement artefacts. The RF-/ACPA+ and the RF+/ACPA+ groups had significantly (p <0.001) larger erosions compared to the RF+/ACPA- and RF-/ACPA- groups. Interestingly, erosions in the RF-/ACPA+ group were even larger than in the RF+/ACPA+ group (p <0.001). Inter-observer reliability (SF, CH) for erosion numbers, widths, depths and volumes was high (mean ICCs: 0.988, 0.977, 0.933, 0.970 and 0,961). Conclusions ACPA-positivity is associated with larger bone erosions in RA-patients. Thus, ACPA essentially contribute to bone loss in RA by enhancing osteoclast activity, which is the key prerequisite for bone erosion. Moreover, ACPA impacts bone erosion independently from presence or absence of RF. References 1. Harre U, Georgess D, Bang H, et al. Induction of osteoclastogenesis and bone loss by human autoantibodies against citrullinated vimentin. The Journal of clinical investigation. 2012 May 1; 122(5):1791-1802. 2. Albrecht A, Finzel S, Englbrecht M, et al. The structural basis of MRI bone erosions: an assessment by microCT. Annals of the rheumatic diseases. 2012 Sep 19. Disclosure of Interest None Declared [1]: #p-6 [2]: #p-7
FRI0488-HPR Are occupational therapy interventions included in the most commonly used european clinical-practice guidelines for the management of osteoarthritis?
Authors: M. Stoffer, D. Taurok, B. Prodinger, J. Smolen, A. Woolf, T. Stamm
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The EUMUSC.net project facilitates cooperation between EU Member States and promotes a comprehensive European strategy to optimise musculoskeletal health. <h3>Objectives</h3> Part of the EUMUSC.net project was devoted to retrieving clinical practice guidelines (CPG) for Osteoarthritis (OA) and to appraise them critically. The purpose of this study was to identify the relevance given to occupational therapy interventions. <h3>Methods</h3> National rheumatological scientific societies, social leagues and health professional associations were contacted and asked to provide relevant documents. Furthermore, a systematic review of the respective literature was conducted in Medline, CINAHL and the Internet. Documents fulfilling pre-defined inclusion and exclusion criteria have been critically appraised by two independent assessors using the AGREE II Instrument. The appraised documents were examined for occupational therapy interventions/occupational therapy (OT). <h3>Results</h3> Six guidelines/recommendations have been included into this study and appraised using the AGREE II Instrument. All six CPGs obtained the highest scores in the domain “Scope and Purpose” followed by remarkably high scores in the domain “Rigor of Development”. Four CPGs had low scores in the domain “Stakeholder Involvement” and five in the domain “Applicability”. We identified ten interventions relevant to occupational therapy: adapting the environment, assistive devices for activities of daily living, hand exercise, joint protection, comprehensive occupational therapy, patient education, self management, splinting/orthoses, thermotherapy and walking assistive devices. Only “patient education” was recommended in all CPGs. “Adapting the environment” in one. <h3>Conclusions</h3> All six guidelines/recommendations addressed occupational therapy interventions. Two CPGs were referring to the term occupational therapy while the others did not. In all appraised documents no occupational therapist could be identified to be a member of an OA Guideline development group. <h3>Disclosure of Interest</h3> None Declared
Abstract ES4-2: The importance of Technical Validation
Authors: JS Reis-Filho
Journal: Cancer Research (2010)

Abstract The development of biomarkers for the diagnosis and classification of breast cancers and stratification of breast cancer patients into clinically meaningful groups is one of the lynchpins of individualised medicine. The correct and robust assignment of patients into subgroups of therapeutic relevance is of utmost importance. In breast cancer management, this is currently performed through the analyses of a few immunohistochemical markers (e.g. oestrogen receptor, progesterone receptor and HER2), fluorescence and/or chromogenic in situ hybridisation, protein analysis of lysates, and quantitative real-time PCR. It has become patently clear, however, that these markers are not sufficient for the potential of individualised therapy to be realised. With the advent of high throughput technologies, fundamental and translational research approaches have provided a wealth of data on diagnostic markers, potential prognostic and predictive markers, and therapeutic targets, which ultimately will need to be incorporated in clinical practice. Some of the major challenges in this process are to determine the accuracy of the research hypothesis, the exclusion of potential biases, and to define whether the reagents and methodologies are fit for purpose. This requires a thorough assessment of the accuracy, robustness and reproducibility of the markers and the methods for their analysis. For instance, immunohistochemistry has become one of the major tools for the identification of expression of potential markers in cancer tissues; albeit at first glance trivial to perform, immunohistochemical analysis can be affected by numerous parameters that can affect its accuracy. Likewise, several approaches for the identification of molecular subgroups by gene expression profiling have been shown not to assign the same patients into the same molecular subgroups consistently. Interpretation of in situ hybridisation is also fraught with difficulties and discrepancies have often been attributed to intra-tumour heterogeneity rather than technical variation. The challenges for the assessment and validation of biomarkers in human tissues and the approaches to overcome them will be discussed. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr ES4-2.
Two clustered cases of confirmed influenza A(H5N1) virus infection, Cambodia, 2011
Authors: N Chea, S D Yi, S Rith, H Seng, V Ieng, C Penh, S Mardy, D Laurent, B Richner, T Sok, S Ly, P Kitsutani, N Asgari, M C Roces, P Buchy, A Tarantola
Journal: Eurosurveillance (2014)

Binary file ES_Abstracts_Final_ECDC.txt matches