tbuckley/cabot-search huggingface dataset:
AB0396 Patients with rheumatoid arthritis – general psychological characteristics
Authors: L. Myasoutova, S. Lapshina
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> To assess psychological characteristics of patients with rheumatoid arthritis (RA). <h3>Methods</h3> 30 patients were included (3 male, 27 female, mean age 56.8 years) with proven diagnosis of RA (according to the 1987 revised ACR criteria). A low disease activity according to DAS 28 score was reported in 6 patients, moderate – in 11 patients and high activity – in 13 patients. Clinical observation, conversation, testing and case history revision were used. Following psychological techniques were used: “Stress intensity” questionnaire, Teylor’s questionnaire, Eydemiller’s “typical family state” methodic, Lüscher color test, “hand” test by Wagner, and author’s questionnaire. <h3>Results</h3> 27% of patients had high level of family anxiety, 67% had mental stress, 83% - high emotional trouble. Vast majority of patients has high level of personal and situational anxiety. Patients with RA try not to react to conflicts, to restrain their emotions, to suppress aggression. Patients’ mental state before and after the onset of the disease, according to the patient’s opinion, have significant differences. Before the onset of the disease 70% of patients considered themselves as sociable, after the onset – only 3%, one third of them identify themselves as closed, one fifth – vulnerable. A correlation was detected between activity of RA and the level of family anxiety, family dissatisfaction, mental stress, the intensity of stress, emotional distress, general anxiety (p&gt;0.01). <h3>Conclusions</h3> Patients with RA were characterized by specific psychological features, including high level of anxiety, emotional trouble, which can be both consequences of RA and provocative factor of RA progression. According to study results, complex medical and psychological aid should be provided to patients with RA, and such methods as individual and group psychotherapy, autogenic therapy, psychological and physiological relaxation can be introduced. <h3>Disclosure of Interest</h3> None Declared
Obstructed Ectopic Kidney Mimicking Chilaiditi Syndrome on F-18 FDG Imaging
Authors: Madhusudhan P. Reddy, Anil Malik, Ramin Zamani, David L. Lilien
Journal: Clinical Nuclear Medicine (2003)

Fluorine-18 fluorodeoxyglucose positron emission tomography was obtained in a patient with a duodenal mass. Conventional imaging revealed multiple dilated intestinal loops. Initial impression of positron emission tomographic images was interposition of a loop of bowel between the liver and diaphragm (Chilaiditi syndrome) in an unusual location, with absent right kidney activity. Computed tomography and magnetic resonance imaging of the abdomen showed an obstructed ectopic kidney in this position with a markedly dilated collecting system. There is absence of fluorodeoxyglucose in the markedly dilated renal collecting system resulting from acute obstruction, giving the false appearance of gas-filled bowel loops with an unusual location of the interposition.
Adeno-Associated Virus Expresses Transgenes in Hair Follicles and Epidermis
Authors: Ulrich R. Hengge, Alireza Mirmohammadsadegh
Journal: Molecular Therapy (2000)

Adeno-associated virus (AAV) vectors are nonpathogenic, integrating DNA vectors capable of transducing dividing and nondividing cells with the potential of long-term expression. Evaluating this interesting vector system in the skin for the first time, we found that an AAV vector containing the lacZ gene (AAVlacZ) led to the expression of β-galactosidase for more than 6 weeks following in vivo injection. Interestingly, expression was present not only in dividing and postmitotic epidermal keratinocytes but also in hair follicle epithelial cells and eccrine sweat glands. However, expression upon readministration was limited. Functional studies in swine using human erythropoietin were hampered by immunogenicity. Thus, AAV seems to be the only vector to date that efficiently targets hair follicle epithelial cells. It may also be useful when longer term expression in keratinocytes than that achievable by direct injection of plasmid DNA is desired. Adeno-associated virus (AAV) vectors are nonpathogenic, integrating DNA vectors capable of transducing dividing and nondividing cells with the potential of long-term expression. Evaluating this interesting vector system in the skin for the first time, we found that an AAV vector containing the lacZ gene (AAVlacZ) led to the expression of β-galactosidase for more than 6 weeks following in vivo injection. Interestingly, expression was present not only in dividing and postmitotic epidermal keratinocytes but also in hair follicle epithelial cells and eccrine sweat glands. However, expression upon readministration was limited. Functional studies in swine using human erythropoietin were hampered by immunogenicity. Thus, AAV seems to be the only vector to date that efficiently targets hair follicle epithelial cells. It may also be useful when longer term expression in keratinocytes than that achievable by direct injection of plasmid DNA is desired.
Abstract 1171: Gastrointestinal stromal tumors (GIST) cell proliferation inhibition with combined adenoviral and molecular targeted therapy
Authors: Derron E. Allen, J. Pablo Arnoletti, Martin J. Heslin, Takahiro Taguchi, Elena A. Kashentseva, Igor Dmitriev, Andrey Frolov
Journal: Cancer Research (2010)

Abstract Introduction: GIST, the most common mesenchymal malignancies of the GI tract, are characterized by gain-of-function mutations in KIT and PDGFRα receptors. Imatinib and sunitinib are tyrosine kinase inhibitors (TKI) currently approved for the treatment of GIST. Recent evidence suggests that GIST cells acquire secondary resistance to TKI through activation of alternative signaling pathways. We hypothesize that gene therapy with replicative vector oncolysis (virotherapy) can be utilized to improve efficacy of TKI and overcome GIST resistance to treatment. Objectives: To determine if a panel of oncolytic adenoviruses in combination with imatinib and sunitinib, results in synergistic inhibition of GIST cell proliferation. Results: GIST882 and GIST-T1 cells were subjected to imatinib and sunitinib treatment in combination with a panel of oncolytic adenoviruses (Ad5, Ad5RGD, Ad5pK7, and Ad5 - Ad3 fiber knob), to assess their inhibitory effects on cell proliferation. The Ad5/3 vector had the best rate of gene transfer and cytolytic effect. The greatest anti-proliferative effects were observed with 100 viral particles/cell of the Ad5/3 vector combined with TKI concentrations of 0.1 µM in GIST-T1 cell line. The combination of TKI and oncolytic adenoviruses showed greater inhibition of GIST cell proliferation (78% decrease) when compared to TKI alone (58% decrease) and viral therapy alone (23% decrease, p&amp;lt;0.01). Successful ex vivo infection and oncolysis of GIST by Ad5/3 particles was confirmed in cells obtained from patient surgical specimens (n=4). Conclusions: The oncolytic adenovirus Ad5/3 infects and inhibits GIST cell proliferation both in vitro and ex vivo. Virotherapy with Ad5/3 has synergistic anti-proliferative effects with TKI and may help overcome GIST resistance to KIT targeted therapy. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1171.
Migration of esophageal self-expandable metal stent to the pleural cavity
Authors: F. Ferreira, R. Gonçalves, F. Vilas-Boas, G. Macedo
Journal: Endoscopy (2011)

A 41-year-old man presented with dysphagia secondary to squamous cell carcinoma of the esophagus, staged as T4N1M0. A covered self-expandable metal stent (SEMS) (Choostent; Solco Intermed, Seoul, Korea) was inserted for symptomatic relief, chemoradiotherapy was performed with carboplatin and 5-fluorouracil, and this was followed by radiotherapy to deliver 39.6 Gy in 22 daily fractions.
Optimal treatment and prognosis of breast cancer after Hodgkin's disease: analysis of 143 cases.
Authors: B Cutuli, C Tunon de Lara, L Livi, M Baron, C Levy, P Kerbrat, M Provencio, L Gonzague-Casabianca, A Mege, C Lemanski, C Delva
Journal: Cancer Research (2009)

Abstract Abstract #6153 Background &amp;#x2028; Breast cancer (BC) is the most frequent second malignancy in women treated for Hodgkin's disease (HD), but there are few specific data on these tumors. This study assesses histopathological features, locoregional and systemic treatments as well as prognostic factors of these secondary BC.&amp;#x2028; Material and methods:&amp;#x2028; 127 women treated for HD in 9 centers subsequently developed 143 BC. Median age at HD diagnosis was 25 years. HD stages were I, II, III and IV in 28%, 49%, 16% and 7%. Radiotherapy (RT) alone was used in 44 cases and chemotherapy (CT) + RT in 83 cases. Twelve patients relapsed and had a new treatment by RT (3), CT (5) or both (4).&amp;#x2028; Results:&amp;#x2028; First BC occurred after 18-year median interval (&amp;lt;10 years: 11%; 10-20 years: 49%; &amp;gt;20 years: 40%). TNM classification (126/143) showed 23 mammographically detected T0 (18%), 54 T1 (43%, 40 T2 (32%) and 9 T3T4 (7%). 129 tumors were infiltrating carcinoma (IC) and 14 (10%) DCIS. Twenty-four (17%) tumors were multifocal. Five patients had metastasis at diagnosis; 135 tumors were operated (121 IC and 14 DCIS): 57 (42%) by conservative surgery (40 with RT) and 78 (58%) by mastectomy (5 with RT). Axillary dissection (AD) and SNB were performed in 74% and 23% of the cases. Median tumor size for IC was 19 mm. Axillary nodal involvement (ANI) was found in 28% of IC (pN1-3 : 22%; pN&amp;gt;3 : 6%). ER and PgR were positive in 57% and 49%.&amp;#x2028; 43 (39%) tumors were SBR III (high grade). Chemotherapy (CT) and hormonotherapy (HT) were performed in 47% and 42% of IC. With a 5-year median FU, 17 patients (12%) developed local recurrences (LR) and 35 (30%) had metastases. Significant metastasis predictive factors were ANI and SBR III; 12 (10%) women developed a third cancer.&amp;#x2028; 5 and 10-year overall survival (OS) rates were 69% and 53%, and disease-specific survival (DSS) rates 72% and 62%. 5-year DSS rates were 79% for T0T1 versus 44% for pT2T3T4 (p=0.0068) and 81% for pN0 versus 65% for pN+ (p=0.0012).&amp;#x2028; Conclusion&amp;#x2028; Young women (especially 15-25 years) treated for HD should be carefully monitored by mammography, ultrasound and maybe MRI in order to detect early occurrence of BC. The prognosis of small lesions (pTis/pT1) is excellent and conservative treatment often feasible. This study is still ongoing, with 200 planned cases. Optimal adjuvant treatment options will be discussed and compared with a former series including 133 cases (Radiotherapy and Oncology, 2001, 59: 247-255). Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 6153.
FRI0484-HPR Individualised patient reported outcomes in arthritis rehabilitation: Evaluation of the patient generated index
Authors: M. Klokkerud, A. Garratt, I. Løchting, I. Kjeken, K.B. Hagen, M. Grotle
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The last decades several individualised patient reported outcome measures have been developed, which allow the patients to choose the items or domains they consider most important. In rehabilitation, treatment is tailored to the patient's individual goals. It has therefore been suggested that patient specific instruments may be better suited to capture the effects of rehabilitation. The Patient Generated Index (PGI) is an individualised instrument designed to measure health related quality of life (HRQoL), which has been used since the early nineties. <h3>Objectives</h3> To evaluate the psychometric properties of the Norwegian version of the Patient Generated Index (PGI) for patients with rheumatic diseases participating in rehabilitation or self-management programmes. <h3>Methods</h3> Patients attending three rehabilitation institutions completed self-report questionnaires (including the PGI) at arrival, 1-2 days after arrival (test-retest), and after 5 and 52 weeks. The PGI, scored from 0 to 100, was assessed for data quality, reliability, validity and responsiveness. Test-retest reliability was assessed by the intraclass correlation coefficient (ICC) and standard error of measurement (SEM)/smallest detectable change (SDC). Construct validity was assessed by testing pre-defined hypotheses of relationships between PGI and the other outcome measures by Pearson correlation. Responsiveness was assessed by the standardised response mean (SRM). <h3>Results</h3> 145 patients aged 29 to 75 years with different inflammatory rheumatic diseases agreed to participate in the study. 118 (81%) filled in the PGI correctly at baseline. Test-retest reliability showed an ICC (95% CI) of 0.86 (0.74, 0.93), a SEM of 7.2 and a SDC of 20.0. Low to moderate correlation was found between the PGI and the SF-36, QoLS, MHAQ and the NRS for pain, fatigue and disease activity. Highest correlations were found between the PGI and the NRS disease activity and the SF-36 role physical (0.47, p&gt;0.001). At 5 weeks the PGI had an SRM of 0.23 and at12 months it was 0.43, which was the highest among the study instruments (figure 1). <h3>Conclusions</h3> The PGI showed good psychometric properties, in particular it showed higher responsiveness at long term than any of the other standardised instruments. A limitation is that relatively many patients found it difficult to fill in the PGI. Further research is needed to increase the feasibility of the PGI. The presence of a facilitator who can guide patients who have problems with completing the PGI or an electronic version with feedback might increase the completion rates. Despite these challenges we recommend the PGI for evaluation of rehabilitation programmes for patients with rheumatic diseases. <h3>Disclosure of Interest</h3> None Declared
Comparison of Plasma Exchange with Plasma Infusion in the Treatment of Thrombotic Thrombocytopenic Purpura
Authors: G A Rock, K H Shumak, N A Buskard, V S Blanchette, J G Kelton, R C Nair, R A Spasoff
Journal: New England Journal of Medicine (1991)

Thrombotic thrombocytopenic purpura is an uncommon disease with a high mortality rate even with current treatment. The cause of the syndrome and its optimal treatment are unknown. Although both plasma exchange and plasma infusion have been useful treatments, it is not clear which is superior. In this report we describe a prospective randomized trial comparing plasma exchange with plasma infusion for the treatment of thrombotic thrombocytopenic purpura.
Abstract 1746: Coordination of overexpressed NIMA-related kinase 2 and TRF1 results in mitotic abnormalities in breast cancer cells.
Authors: Jaehyung Lee
Journal: Cancer Research (2013)

Abstract NIMA-related kinase 2 (NEK2), a serine-threonine protein kinase, plays a role in features of mitotic progression including timing of mitotic entry, chromatin condensation, spindle organization and cytokinesis. Kinase death NEK2 mutant expression or NEK2 depleted cells lead to failure of centrosome separation while NEK2 overexpression results in premature centrosome separation. In addition, it has been revealed that telomeric repeat binding factor 1 (TRF1) interacts directly with NEK2. TRF1 not only regulates telomere length, but is also associated with cell cycle regulation. However, the interactions and correlations between NEK2 and TRF1 are far from clear. Here, we show that mitotic aberrations through NEK2 overexpression are likely to require TRF1. Our results demonstrate that NEK2 directly binds and phosphorylates TRF1 in vitro and in vivo through multiple sites on TRF1. NEK2 overexpression in breast cancer cells, MCF 7 and MDA-MB-231, results in increased numbers of centrosomes and multinucleated cells, which leads to cytokinetic failure and aneuploidization. Additionally, TRF1 depletion by siRNA prevents the occurrence of unaligned chromosomes by NEK2 overexpression during metaphase. Concurrent Nek2 overexpression and TRF1 depleted cells demonstrated ≤ 2 centrosomes per cell, similar to mock plasmid and negative control siRNA transfected cells. Therefore, we propose that TRF1 is required for overexpressed NEK2 to trigger abnormal mitosis and chromosomal instability. Citation Format: Jaehyung Lee. Coordination of overexpressed NIMA-related kinase 2 and TRF1 results in mitotic abnormalities in breast cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1746. doi:10.1158/1538-7445.AM2013-1746
Myocardial Infarction and Mortality in the Coronary Artery Surgery Study (CASS) Randomized Trial
Journal: New England Journal of Medicine (1984)

The long-term benefit of coronary bypass surgery in terms of longevity and prevention of major ischemic events in patients who have mild angina is not well defined. The randomized Coronary Artery Surgery Study (CASS) was designed to evaluate this issue; it consists of 780 patients who were considered operable and who had mild stable angina pectoris or who were free of angina after infarction. As a result of the randomization process there were no significant differences in base-line variables between patients randomly assigned to medical and to surgical therapy. The likelihood of death in the five-year period after randomization was only 8 per cent in the medical cohort, as compared with 5 per cent in the surgical cohort (not significant). The likelihood of nonfatal Q-wave myocardial infarction was 11 and 14 per cent, respectively (not significant). The five-year probability of remaining alive and free of infarction was 82 per cent in the patients assigned to medical therapy and 83 per cent in the patients assigned to surgery (not significant). There were no statistically significant differences in the survival rate or in the myocardial-infarction rate between subgroups of patients randomly assigned to medical and to surgical therapy when they were analyzed according to initial group assignment, number of diseased vessels, or ejection fraction. Therefore, as compared with medical therapy, coronary bypass surgery appears neither to prolong life nor to prevent myocardial infarction in patients who have mild angina or who are asymptomatic after infarction in the five-year period after coronary angiography.
SAT0352 Frequency of fractures and vitamin D serum levels in a cohort of HIV-1 infected patients
Authors: D. Ernst, M. Stoll, R.E. Schmidt, W. Torsten, D. Meyer-Olson
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> An increased risk of hypovitaminosis D and/or fractures in HIV-infected patients has been discussed. We investigated the frequency of fractures and vitamin D serum levels in HIV-infected patients in a single university-based out-patient cohort in Germany. <h3>Methods</h3> Charts of 1098 HIV-infected patients attending our out-patient clinic between 1/2004 and 06/2011 were screened for the presence of fracture at any location. Demographics and the presence of osteoporosis were then evaluated in these fracture patients. Measurement of the serum concentration of 25-OH- vitamin D3 was performed in 115 HIV-1 patients and 110 patients with Spondyloarthritis [SpA]. <h3>Results</h3> In 71 out of 1098 HIV patients (6.4%) a fracture was diagnosed. Most experienced at least one fracture of the lower limb (29,6%). The majority of these were located in the foot or ankle (66.7%). Other common fractures sites included the spine (16.9%) and the upper limb (15.5%). Average age at fracture was 51.84 years (±14.2 stdev). Recurrent fractures occurred in 11.3% of patients. Fractures after negligible or minor trauma occurred in 15 patients at a mean age of 56.9 years (±13.6 stdev). Diagnosis of osteoporosis occurred in 7 out of 71 patients (9.9%) with a mean age of 59.1 years (±6,46 stdev). Four out of seven patients with osteoporosis were below 55 years and five out of seven were male. Decreased levels of 25-OH-vitamin D3 were detected in 81.2% of HIV-1 patients as compared to 61.8% of spondylarthritis patients (p=0.001). <h3>Conclusions</h3> There is a high prevalence of fractures at different locations (most frequently the lower extremity) in HIV-1 infected patients. Fractures after a minor trauma and recurrent fractures were frequently observed in this cohort. HIV-1 seropositive patients osteoporosis was frequently diagnosed in males at a young age. In addition, we observed a higher prevalence of decreased 25-OH- vitamin D3 serum levels in this cohort of HIV-infected patients as compared to patients with spondyloarthritis. <h3>Disclosure of Interest</h3> None Declared
P268 Effect of CPAP Treatment For OSA on Visual Processing of Degraded Words
Authors: K Proudlove, R Hall, R Rieu, M Villarroel, J Stradling
Journal: Thorax (2012)

<h3>Introduction</h3> It has been shown in a 6 month non-randomised clinical trial that patients with both diabetic macula oedema and obstructive sleep apnoea (OSA) improved vision by one line on the logMAR chart if they used CPAP for more than 2.5 hours/night, compared to those using for less than 2.5 hours/night. However there are many potential explanations for this improvement, including reduced sleepiness leading to increased effort to read the logMAR chart, rather than any direct improvement in retinal function. We are assessing if non-visually impaired patients, treated for OSA, also improve their ability to read visually degraded words, thus simulating impaired vision. <h3>Methods</h3> A specially designed computer programme repeatedly presents five-letter words for three seconds, with one second intervals between. Progressively, the bottom halves of the letters making up the words are removed, making them harder and harder to read. The point at which only 50% of the words are correctly recognised is ‘hunted’ by increasing and decreasing the % revealed, analogous to a hearing test that hunts the auditory threshold. Preliminary tests on normal subjects established that a 10 minute training period, followed by a 10 minute rest, and then by a 10 minute trial period, produced stable results with little or no learning effect over several weeks. 23 symptomatic patients with OSA have been tested pre CPAP, and so far only 5 have been re-tested approximately 2 weeks post CPAP. <h3>Results</h3> The average % of words needing to be revealed to allow 50% correct recognition in the 23 subjects was 35% (range 23 to 64%). In the 5 restudied so far this was 37% pre CPAP, and 40% post. <h3>Conclusions</h3> Although the study is not yet finished, the preliminary results are not supporting the hypothesis that improved vision in patients with diabetic macular oedema post CPAP treatment is likely to be due to reduced sleepiness, improved vigilance or improved enthusiasm for reading the logMAR chart.