tbuckley/cabot-search huggingface dataset:
Quality of life, depressed mood, and anxiety in women presenting with locally advanced breast cancer
Authors: D. B. Jeffe, M. J. Naughton, K. N. Weilbaecher, M. A. Ali, R. L. Aft
Journal: Journal of Clinical Oncology (2004)

8249 Background: Despite advances in breast cancer screening, the rate of women presenting with locally advanced breast cancer (LABC) has remained unchanged for the past 10 years. These women are at greater risk of dying from their disease. We hypothesize that psychosocial factors (e.g., depression and anxiety) may have an impact on their quality of life over and above the effects of treatment. Methods: We completed one-on-one interviews with clinical stage II or III breast cancer patients prior to start of breast cancer treatment. Quality of life (QOL) using the FACT-B as well as demographic variables and symptoms of depressed mood and anxiety using the Center for Epidemiologic Studies Depression Scale and Beck Anxiety Inventory, respectively, were assessed. Results: With a targeted accrual of 40 patients in the first year (120 over 3 years), 34 have been enrolled in 10 months. The sample is 70.6% white (2.9% Hispanic), 23.5% African American, 5.9% Asian Indian or Pakistani, 70.6% married, 58.8% employed at least part time, 50% with more than high school education, and mean age of 52 years (range 38–68). On average, patients reported moderately high levels of QOL (mean +/- SD 109.8 +/-18.8; possible high score=144), little or no depressed mood (11.7 +/- 9.1; possible high score=60), and mild-moderate anxiety (10.9 +/- 6.6; possible high score=63). However, 35% reported levels of depressed mood higher than 16, a cut-off score consistent with that of clinically depressed patients, and 59% reported at least mild-moderate anxiety (scores 10–18, with non-clinical samples reporting <10). Depressed mood and anxiety were correlated with QOL (each p < 0.001). Conclusions: One-third of newly diagnosed LABC patients reported symptom scores consistent with clinical depression, and over half reported mild-moderate anxiety -each higher than levels found in non-clinical populations. In addition to being correlated with QOL, anxiety and depression might contribute to delay in diagnosis as well as affect treatment compliance. Thus, psychosocial adjustment of women with LABC should and will be monitored throughout their breast cancer treatment. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Novartis; Pfizer
Abstract 1889: Paleolithic and Mediterranean diet pattern scores and their associations with biomarkers of inflammation and oxidative balance
Authors: Kristine Whalen, Marji McCullough, W. Dana Flanders, Terryl J. Hartman, Suzanne Judd, Roberd M. Bostick
Journal: Cancer Research (2015)

Abstract Chronic inflammation is associated with poor diet quality as well as risk for cancer and other chronic diseases. A diet-inflammation association may relate to evolutionary discordance. We investigated associations of two diet pattern scores, the “Paleolithic” and the Mediterranean, with circulating levels of two inflammation-related biomarkers, C-reactive protein (CRP), an acute inflammatory protein, and F2-isoprostanes, a reliable marker of lipid peroxidation in vivo, in a colonoscopy-based, pooled case-control study of incident sporadic colorectal adenomas (N = 646). We created diet scores from responses on a modified Willett food-frequency questionnaire, and measured plasma levels of CRP and F2-isoprostanes by ELISA and GCMS, respectively. Both diet scores were calculated and categorized into quintiles, and their associations with the biomarker levels were estimated using unconditional logistic regression. The multivariable-adjusted odds ratios (OR) comparing those in the highest relative to those in the lowest quintiles of the Paleolithic and Mediterranean diet scores, were, respectively, 0.73 (95% confidence interval [CI] 0.39, 1.36; Ptrend = 0.35) and 0.71 (95% CI 0.36, 1.40; Ptrend = 0.12) for CRP levels, and 0.35 (95% CI 0.17, 0.74; Ptrend &amp;lt;0.01) and 0.64 (95% CI 0.29, 1.40; Ptrend = 0.03) for F2-isoprostanes levels. These findings, which are particularly strong for the inverse Paleolithic diet score-F2-isoprostanes association, suggest that diets that are more “Paleolithic-” or Mediterranean-like may be associated with lower levels of systemic inflammation and oxidative stress. Citation Format: Kristine Whalen, Marji McCullough, W. Dana Flanders, Terryl J. Hartman, Suzanne Judd, Roberd M. Bostick. Paleolithic and Mediterranean diet pattern scores and their associations with biomarkers of inflammation and oxidative balance. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 1889. doi:10.1158/1538-7445.AM2015-1889
PTH-022 Single balloon enteroscopy: initial experience from a UK tertiary centre
Authors: S D McLaughlin, D Borrow, S H C Anderson
Journal: Gut (2010)

<h3>Introduction</h3> Single balloon enteroscopy (SBE) potentially allows assessment of the entire small bowel with the possibility for therapeutic intervention. Preliminary data suggest that this may be better tolerated than double balloon enteroscopy.1 There is limited UK experience of this novel endoscopic technique and we report the initial outcomes from our unit. <h3>Aim</h3> To assess the indications, diagnostic and therapeutic yield, tolerance and complications of SBE performed within a single tertiary unit. <h3>Methods</h3> A prospective database is maintained at our unit. We reviewed the database entries and case notes of all patients who underwent SBE over a 24-month period. Comfort and sedations scores are recorded by the nursing staff. Comfort; 1=no discomfort/unable to remember, 2=slightly uncomfortable, 3=uncomfortable but tolerable 4=brief periods of significant discomfort, 5=unacceptable. Sedation; 1=excellent, 2=good, 3=poor. <h3>Results</h3> Video capsule endoscopy was performed in all patients before SBE. Thirty-seven SBE procedures were performed on 26 patients (20 males), mean age; 64 (range 29–83). Indications were: Iron deficiency anaemia (4), obscure gastrointestinal (GI) bleeding (18), suspected Crohn9s disease (2), abnormal radiology (2), abnormal video-capsule endoscopy findings (10). Thirty-two were antegrade and five were retrograde procedures. 16 (42%) procedures were therapeutic. Histological biopsies were taken in 6 (17%). Two patients required general anaesthesia (1 had a previously failed SBE). Mean sedation doses were midazolam: 4.8 mg (range 2.5–10), fentanyl 85.7 μg (range 50–100). Mean comfort score=2 (range 2–5), mean sedation score 2 (range 2–3). Overall diagnostic yield was 61%. Diagnoses: Angiodysplasia n=10, normal n=15, ulceration n=7, Crohn9s disease n=1, Other n=5. Complications: none. <h3>Conclusion</h3> Our experience demonstrates that the majority of patients can tolerate SBE under conscious sedation with acceptable comfort and sedation scores. SBE appears to be a safe procedure in all age groups. It has a high diagnostic yield and is a useful modality for therapeutic intervention particularly in obscure GI bleeding.
Significant in Vivo Efficacy of the SINE KPT-330 in Mouse Models of CLL.
Authors: Rosa Lapalombella, Virginia Goettl, Katie Williams, Larissa Tangeman, Shruti Jha, Jason A Dubovsky, Danielle L Chappell, Amy J Johnson, Vincent Sandanayaka, Sharon Shechter, Dilara McCauley, Sharon Shacham, Michael Kauffman, John C. Byrd
Journal: Blood (2012)
Abstract 4948: The cytosolic domain of a disintergrin and metalloprotease (ADAM) 15 promotes non-small cell lung cancer (NSCLC) proliferation
Authors: Hsin-Han Hou
Journal: Cancer Research (2015)

Abstract Emerging evidence has indicated that proteins of a disintergrin and metalloprotease (ADAM) family contribute to cancer progression and metastasis. One member of this family, ADAM15, was reported to over-express in multiple cancers, including gastric, lung, breast, and prostate cancers. It has been shown that the enzymatic activities of its extracellular metalloprotease domain mediate ErbB signaling pathway and promote breast cancer proliferation and migration. Nevertheless, in non-small cell lung cancer (NSCLC), the role of ADAM15 and its correlation to patient prognosis is still not well understood. Moreover, unlike in breast cancer, the enzymatic activities of the ADAM15 extracellular domain appeared to be independent of ErbB signaling and lung cancer proliferation, indicating an alternative mechanism of ADAM15 in promoting lung cancer progression. Here, we demonstrated that ADAM15 was over-expressed in primary NSCLC tissues compared to the adjacent normal tissues. Patients with ADAM15 high-expressing lung tumors had shorter survival times. Furthermore, we examined different isoforms of ADAM15 and discovered that, isoform 6 (i6), which contains the most cytoplasmic Src homology 3 (SH3) binding motifs, was significantly upregulated in primary lung cancer tissues. Comparison of i6 and isoform 1 (i1), which has no cytoplasmic SH3 binding motifs, revealed that the cytoplastmic domain is essential for lung cancer proliferation, as well as aggressiveness in an in vitro lung cancer migration model. We also showed that ADAM15 i6, but not i1, mediated ErbB signaling pathway through physical interaction with growth factor receptor-bound protein 2 (Grb2), and activation of Src homolog 2 domain containing (Shc). Most importantly, overexpression of ADAM15 i6 promoted NSCLC cell growth in a xenograft mouse model while knockdown of i6 resulted in tumor shrinkage. Thus, we identified a novel mechanism of the ADAM15 cytoplasmic domain in NSCLC tumor progression, which will not only shed light on the molecular mechanisms of ADAM proteins, but also facilitate development of novel therapy in NSCLC. Citation Format: Hsin-Han Hou. The cytosolic domain of a disintergrin and metalloprotease (ADAM) 15 promotes non-small cell lung cancer (NSCLC) proliferation. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4948. doi:10.1158/1538-7445.AM2015-4948
Evaluation of 1p and 19q chromosomal alterations in oligodendroglial neoplasms using microdissection and microsatellite analysis technique and correlations with response to chemotherapy
Authors: E. Franceschi, G. Cavallo, A. Paioli, L. Scopece, R. Degli Esposti, E. Leonardi, F. Spagnolli, A. M. Cremonini, A. Pession, L. Crino
Journal: Journal of Clinical Oncology (2004)

1568 Background: Oligodendroglial tumors are chemosensitive diseases. The evaluation by fluorescence in situ hybridization has been used to assess 1p and 19q alterations that are considered the best chemosensitivity test in these tumors. Here we show another way to evaluate 1p and 19q chromosomal alterations by using microsatellite analysis that could be considered an useful tool in translational research for the accuracy of this method. Methods: Patients DNA was extracted from peripheral blood to define the wild type allelic condition, instead tumoral DNA was obtained from laser microdissected cells. We analized 4 microsatellites on Chr1p and 3 on Chr19q (chosen from sequences described by J.M. Nigro et al., Am J Path 2001); for each locus loss of heterozigosity (LOH), allelic imbalance or chromosomal instability were defined based on allelic peaks pattern. Chemotherapic regimens allowed were: PCV or Temozolomide or Carboplatin-Etoposide (CE). Results: At the time of abstract submission we evaluated 21 oligodendroglial tumor adult patients: 4 with oligodendroglioma, 12 with anaplastic oligodendroglioma and 5 with mixed oligoastrocytoma (2 G2 and 3 G3). In 10 patients both primary and recurrent disease samples were available. 16/21 patients were treated with chemotherapy. PCV combination was used as first line in 1 patient, Temozolomide was used as first line in 9 patients and CE combination was used as first line in 6 patients. Conclusions: The evaluation of 1p and 19q status by microsatellite analysis is feasible and provides useful informations about oligodendroglial tumors. Further results and correlations with response to radiotherapy and chemotherapy, and differences in 1p and 19q LOH pattern between primary and recurrence will be presented at the meeting. No significant financial relationships to disclose.
Role of the Chemokine Receptor CXCR4 in Waldenstrom Macroglobulinemia.
Authors: Irene M. Ghobrial, Mona Melhem, Ujjal Singha, Diane George, Michael Timm, Lanie Francis, Yazan Alsayed, Alissa Huston, G. David Roodman, Philip Greipp, Morie Gertz, Thomas Witzig
Journal: Blood (2005)
Abstract 986: Role of Gpr43 in intestinal inflammation and carcinogenesis
Authors: Sathish Sivaprakasam, Ashish Gurav, Ganapathy Vadivel, Nagendra Singh
Journal: Cancer Research (2015)

Abstract Consumption of dietary fiber is positively correlated with lower incidence of inflammation and carcinogenesis in intestine. In colon dietary fibers are fermented by gut microbiota into short chain fatty acids (SCFA, acetate, propionate and butyrate). Several studies demonstrate that SCFA play a key role in protection of intestine from inflammation and carcinogenesis. However, detail molecular mechanisms underlying the biological effects of SCFA on intestine health are not fully understood. Gpr43 is cell surface receptor for SCFA and recent evidences suggest that it regulates colonic inflammation in a context dependent manner. Intestinal inflammation is one of the risk factor for development of cancer in gut. Based on these findings, role of Gpr43 in regulation of colon carcinogenesis was evaluated. Colitis associated colon cancer in wild type (WT) and Gpr43-/- mice was induced by single intraperitoneal injection of azoxymethane (AOM) and repeated cycles of dextran sulfate sodium (DSS) in drinking water. Gpr43-/- mice showed drastically reduced survival, severe weight loss and increased diarrhea and rectal bleeding than WT mice. At the end of treatment, colons of Gpr43-/- mice exhibited increased number of polyps than that of WT mice. Reciprocal bone marrow chimera experiments revealed that Gpr43 expression in non-hematopoietic cells play a major role in suppression of colonic inflammation and carcinogenesis. The data presented here suggest a model in which Gpr43 plays a critical role in facilitating positive effects of dietary fiber and gut microbiota on intestinal health. Citation Format: Sathish Sivaprakasam, Ashish Gurav, Ganapathy Vadivel, Nagendra Singh. Role of Gpr43 in intestinal inflammation and carcinogenesis. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 986. doi:10.1158/1538-7445.AM2015-986
A Phase I Study of Everolimus in Combination with Time-Intensified DHAP (Dexamethasone, High-Dose AraC [Cytarabine], Cisplatinum) in Patients with Relapsed or Refractory Classical Hodgkin Lymphoma (cHL)
Authors: Bastian von Tresckow, Annette Pluetschow, Heinz Haverkamp, Vladan Vucinic, Andreas Rank, Max S. Topp, Michaela Feuring-Buske, Carsten Kobe, Georg Kuhnert, Boris Böll, Dennis A. Eichenauer, Stephanie Sasse, Michael Fuchs, Andreas Engert, Peter Borchmann
Journal: Blood (2014)
Abstract 3202: Single cell analysis of ascites macrophages in ovarian cancer
Authors: Huanhuan Mahsa He, Feiqiao Brian Yu, Stephen R. Quake, Oliver Dorigo
Journal: Cancer Research (2015)

Abstract Macrophages compose a major part of the tumor microenvironment (TME) in several types of cancer, including ovarian cancer [1]. Studies have shown that a high percentage of tumor associated macrophages (TAMs) correlates with a poor prognosis in ovarian cancer [2], due to the crucial role of TAMs in promoting tumor growth and angiogenesis [3,4]. Yet not much investigation has been executed to reveal the function of macrophages in ovarian cancer ascites, a hallmark of ovarian cancer. Previous gene expression analysis in TAMs often used bulk samples [5,6]. However, due to the complexity of the peritoneal environment, this approach had the effect of “dampening” rare and potentially informative signals from a heterogeneous population such as ascites macrophages. Therefore, we resorted to single-cell expression analysis to dissect the heterogeneity of ascites macrophages. Recent breakthroughs in single cell RNA-seq techniques have made it more cost effective to process a large number of samples at single cell resolutions. Here we used Fluidigm's Single-Cell Auto Prep System, a state-of-art technology for automate single cell cDNA synthesis [7]. We sequenced single ascites macrophages from ID8 murine ovarian cancer model and single alternatively activated bone marrow derived macrophages (BMDM-M2). When comparing the single cell expression profiles of ascites macrophages to BMDM using principle component analysis (PCA), we observed that the ascites macrophages scattered broadly and could be further divided into two groups, whereas BMDM were tightly clustered into one group. Furthermore, gene oncology (GO) analysis of highly expressed genes in each group revealed that ascites macrophages had a distinct gene expression profile from BMDM, highlighting the metabolic pathways, especially catabolic process. We are currently working on validating these findings in human patients with ovarian carcinoma. In summary, this work sheds light on the understanding of the role of ascites macrophages in ovarian cancer. 1. Qian BZ, Pollard JW. Cell. 2010;141:39-51. 2. He YF, Zhang MY, Wu X, et al. PLoS One. 2013;8:e79769. 3. Mantovani A, Germano G, Marchesi F, et al. Eur J Immunol 2011;41:2522-25. 4. Murray PJ, Wynn TA. Nat Rev Immunol, 2011;11:723-37. 5. The Cancer Genome Atlas Research Network. Nature. 2014;511:543-50. 6. The Cancer Genome Atlas Research Network. Nature. 2014;513:202-9. 7. Wu AR, Neff NF, Kalisky T, et al. Nat Methods. 2014;11:41-6. Citation Format: Huanhuan Mahsa He, Feiqiao Brian Yu, Stephen R. Quake, Oliver Dorigo. Single cell analysis of ascites macrophages in ovarian cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3202. doi:10.1158/1538-7445.AM2015-3202
Increased blood levels of NKG2D+CD4+ T cells in patients with alopecia areata
Authors: Yong Hyun Jang, Jin Kyeong Choi, Yun Hwan Jang, Sun Young Moon, Weon Ju Lee, Seok-Jong Lee, Young-Ae Choi, Sang-Hyun Kim, Do Won Kim
Journal: Journal of the American Academy of Dermatology (2016)
471. Cell Incubation Outside of a Barrier Isolator Using an Improved Conditioned Transport Chamber Expands Scale-Out Capacity for Massively Parallel Patient-Specific Batch Production
Authors: Alicia D. Henn, Shannon Darou, Randy Yerden
Journal: Molecular Therapy (2015)

Massively parallel patient-specific batch production of cellular therapeutics, such as in personalized CAR-T cell production for leukemia therapies, presents major technical challenges. Risks of contamination are reduced when a barrier isolator is used to surround the cellular production processes with a protective work tunnel. However, finite incubation space can limit scale-out of operations. In this study, we used an improved version of a Transportable Conditioned Cell Culture Chamber™ (TC4™) to extend the protected environment of the barrier isolator to cultures transported to an outside incubator. We conducted cell growth kinetic studies using K562 cells, a cell line often used for optimizing T cell bioprocess operations. Cultures were incubated in three separate TC4™ in external incubators, mimicking three separate patient-specific CAR-T cell cultures. Twice weekly over four weeks, cultures were moved into the isolator for systematic processing. The environment of each TC4™ chamber was conditioned by the barrier isolator before being returned to the exterior incubator. We found no statistical differences between the growth of K562 cells grown using the TC4™ chambers and those incubated inside the barrier isolator full-time. Our disinfection protocol was effective at prevention of contamination of the barrier isolator by entering TC4™ chambers. We conclude that the improved transportable chambers can extend the risk-reduction of the barrier isolator to external incubators, providing vastly expandable parallel cellular therapy production capacity.
389. Assessment of Sensorimotor Skills of the MCK Model of Friedreich Ataxia
Authors: Mohammed Filali, Robert Lalonde, Catherine Gérard, Jacques P. Tremblay
Journal: Molecular Therapy (2015)

Friedreich ataxia (FA) is the most common autosomal recessive disorder of the cerebellum, causing degeneration of spinal sensory neurons and spinocerebellar tracts. The disease is caused by severely reduced levels of frataxin, a mitochondrial protein involved in iron metabolism. We tested an experimental model generated by Dr. Puccio's group by crossing mice homozygous for a conditional allele of the Fxn gene with mice heterozygous for a deleted exon 4 of Fxn carrying a tissue-specific Cre transgene under control of the muscle creatine kinase promoter. Relative to wild-type, the MCK-Cre conditional mutants were impaired on tests of motor coordination comprising horizontal bar, vertical pole, and the rotorod as well as displaying gait anomalies and the hindlimb clasping response. Thus this MCK-Cre model reproduces some key features of muscle dysfunction in patients with Friedreich ataxia and provides an opportunity of ameliorating their symptoms with experimental therapies.
Long-Term Outcome of AIDS-Related Lymphoma Treated with Abbreviated Cycles of EPOCH-RR: A Prospective Study of 40 Patients
Authors: Kieron Dunleavy, Richard Little, Alan S. Wayne, Nicole Grant, Stefania Pittaluga, Elaine S. Jaffe, Seth Steinberg, Robert Yarchoan, Jorge A. Carrasquillo, John E. Janik, Wyndham H. Wilson
Journal: Blood (2008)