tbuckley/cabot-search huggingface dataset:
Studies on the Specificity of Human Cytotoxic Antibody Reactive With Cultures of Lymphoid Cells
Authors: R. B. Herberman, M.D.
Journal: JNCI Journal of the National Cancer Institute (1969)

Studies are reported on an antigen of human cells demonstrated by reactions with a cytotoxic antibody in human serum. Quantitative determination of the antigen was performed by absorption of antibody-containing serum before reaction with the human lymphoid tissue culture cells. The absorption studies indicated that the antigen was present on all of 19 human lymphoid cell lines established from patients with a variety of diseases and from normal adults. Cell lines derived from patients with Burkitt's tumor contained the highest quantity of antigen; those insensitive to the cytotoxic antibody had fewer antigenic determinants. Peripheral blood leukocytes also contained the antigen in low concentration. Absorption experiments were performed with autochthonous leukocytes to rule out the possibility that isoantibodies were contributing to the cytotoxic activity. The individual's own leukocytes could absorb the cytotoxic activity from his own serum. Antigenic activity was also demonstrated in spleen, bone marrow, liver, kidney, and brain. Erythrocytes had no detectable antigenic activity. These studies indicate that the antigen is neither organ-specific nor tumor-specific.
Preoperative Amiodarone as Prophylaxis against Atrial Fibrillation after Heart Surgery
Authors: Emile G. Daoud, S. Adam Strickberger, K. Ching Man, Rajiva Goyal, G. Michael Deeb, Steven F. Bolling, Francis D. Pagani, Cynthia Bitar, Marc D. Meissner, Fred Morady
Journal: New England Journal of Medicine (1997)

Atrial fibrillation occurs commonly after open-heart surgery and may delay hospital discharge. The purpose of this study was to assess the use of preoperative amiodarone as prophylaxis against atrial fibrillation after cardiac surgery.
Thickening of Walls of Arterioles and Small Arteries in Relation to Age and Smoking Habits
Authors: Oscar Auerbach, E. Cuyler Hammond, Lawrence Garfinkel
Journal: New England Journal of Medicine (1968)

In five independent studies fibrous thickening of the arterioles and arteries were studied microscopically in men who died and came to autopsy. The locations were the trachea and main bronchi, the esophagus, the stomach wall, the pancreas and the adrenal glands. One specimen each from 925 to 1025 subjects taken at autopsy were studied for the adrenal gland, stomach wall and pancreas. A total of 8764 esophageal sections from 918 subjects and 5566 tracheobronchial sections available from previous studies of epithelial changes were also independently studied for vascular thickening. The microscopical findings were analyzed according to smoking habits and by age. It was found that in all five locations the degree of fibrous thickening tended to increase with age, tended to be considerably greater in cigarette smokers than in nonsmokers and tended to increase with amount of cigarette smoking.
Association of Epicardial Fat Thickness with TIMI Risk Score in NSTEMI/USAP Patients
Authors: Fırat Özcan, Osman Turak, Uğur Canpolat, Selçuk Kanat, Iskender Kadife, Sedat Avcı, Ahmet Işleyen, Muhammed Cebeci, Derya Tok, Fatma Nurcan Başar, Dursun Aras, Serkan Topaloğlu, Sinan Aydoğdu
Journal: Journal of the American College of Cardiology (2013)
AB1029 Genistein prevents quality of life osteopenic post-menopausal womens: A 2-year randomized, double-blind, controlled-study
Authors: M. Atteritano, G. Miceli, G. Bagnato, D. Sangari, R. Talotta, A. Tamburello, S. Morgante, G. Bagnato
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Postmenopausal estrogen decline is implicated in several age-related physical and psychological changes in women, including decreases in perceived quality of life (QoL). A number of trials with hormone therapy showed beneficial effects of the intervention on parameters of quality of life. However, because of known or suspected serious side-effects of conventional hormone therapy there is a need for alternatives <h3>Objectives</h3> The goal of this study was to evaluate the effect of genistein treatment on health-related quality of life in osteopenic postmenopausal women. <h3>Methods</h3> We conducted a double-blind randomized placebo-controlled trial with phytoestrogen genistein 54 mg or placebo for 2 year. For this trial, we recruited 288 postmenopausal women aged 49 to 67 years. At baseline, after 1 year and at final visit, participants filled in the Short Form of 36 questions (SF-36), the Questionnaire on Life Satisfaction Modules (QLS<sup>M</sup>), and the Zung Self-Rating Depression Scale (ZSDS). <h3>Results</h3> For the placebo group scores on all dimensions of the SF-36 and the QLS<sup>M</sup> decreased after 1 year and after 2 years. The genistein group showed increases on all dimensions of the SF-36 and the QLSM. There were however statistically significant differences in changes of scores between the two intervention groups. For the ZSDS similarly, significant differences were found between the groups. <h3>Conclusions</h3> In conclusion, the findings in this randomized trial do support the presence of a marked effect of phytoestrogen genistein on quality of life (health status, life satisfaction, and depression) in osteopenic postmenopausal women. <h3>References</h3> Herschbach P, Henrich G, Strasburger CJ, et al. Development and psychometric properties of a disease-specific quality of life questionnaire for adult patients with growth hormone deficiency. Eur J Endocrinol 2001;1453:255–265. Blumel JE, Castelo-Branco C, Binfa L, et al. Quality of life after the menopause: a population study. Maturitas 2000;341:17–23. Ravnikar VA. Compliance with hormone replacement therapy: are women receiving the full impact of hormone replacement therapy preventive health benefits? Women Health Iss 1992;22:75–80. Writing Group for the Women’s Health Initiative Investigators (2002) Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results From the Women’s Health Initiative randomized controlled trial. JAMA 288 (3):321–333 Zung, WW (1965). A self-rating depression scale. Archives of General Psychiatry 12: 63–70. <h3>Disclosure of Interest</h3> None Declared
Abstract 5435: Increased tumor susceptibility to low glucose under hypoxia is increased by BRAF(V600E) mutation: correlation with persistent phosphoP90RSK activation.
Authors: Manuel Rieber, Valery Chavez-Perez, Mary Strasberg-Rieber
Journal: Cancer Research (2013)

Abstract BACKGROUND.- Oncogenic BRAF(V600E) mutations, found in &amp;gt;50 % of melanomas, promote melanoma cell proliferation by constitutively activating mitogen-activated Mek1/2 kinases and extracellular signal-regulated kinases (Erk1/2). This pathway can further activate downstream the p90- ribosomal S6 kinase (p90RSK). OBJECTIVES.- Since BRAF mutations increase melanoma progression which could lead to metabolic stress when oxygen, glucose, and other nutrients are limiting within poorly vascularized regions, we investigated whether hypoxia and metabolic stress can differentially influence the survival of human melanoma cells harbouring either wt or oncogenic BRAF. EXPERIMENTAL. human melanoma cells with unequal BRAF status were exposed to glucose and/or glutamine limitations under normoxia or hypoxia. Relative metabolic activity was fluorometrically quantitated by the cellular conversion of resazurin to resorufin. Differential cell survival was assesed by crystal violet staining. Mechanisms influencing the response to metabolic stress were studied by specific immune blotting. RESULTS. the metabolic activity and survival of melanoma cells harbouring oncogenic BRAF was preferentially decreased under hypoxia at sub-physiological glucose, compared to wt BRAF melanoma cells. Addition of the anti-oxidant N-acetylcysteine or pyruvate enhanced the metabolic activity and survival of wt BRAF cells, but these were much less effective when added to BRAF (V600E) mutant cells, implying a greater oxidative stress induced by low oxygen and glucose in them. Susceptibility to metabolic stress under hypoxia and low glucose in BRAF (V600E) mutant cells, correlated with persistent (p90RSK) activation but lower GLUT-1 and MnSOD expression. In contrast, wt BRAF melanoma cells resistant to metabolic stress reciprocally showed higher GLUT-1 and MnSOD expression, but negligible p90RSK activation. CONCLUSION.- Although mutations in BRAF can drive acquired resistance to MEK1/2 inhibitors, our results suggest that restricting glucose consumption in hypoxic melanomas harbouring mutant BRAF with persistent phosphorylation of the p90-ribosomal S6 kinase, may contribute to their preferential killing. Citation Format: Manuel Rieber, Valery Chavez-Perez, Mary Strasberg-Rieber. Increased tumor susceptibility to low glucose under hypoxia is increased by BRAF(V600E) mutation: correlation with persistent phosphoP90RSK activation. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5435. doi:10.1158/1538-7445.AM2013-5435
SP0004 The Old and the New in the Treatment of GIANT Cell Arteritis
Authors: M.A. Gonzalez-Gay
Journal: Annals of the Rheumatic Diseases (2014)

<h3></h3> Giant cell arteritis (GCA), also called temporal arteritis, is a large- and medium-sized blood vessel systemic vasculitis characterized by the granulomatous involvement of the aorta and its major branches. This vasculitis mainly occurs in white individuals older than 50 years. Due to the progressive aging of the population in Western countries, GCA has emerged as relatively common disease in the elderly. Clinical manifestations of GCA are the result of inflammation of the arterial wall, leading to a series of structural changes, such as intimal hyperplasia and fragmentation of internal elastic laminae and luminal occlusion. Headache is the most typical and common clinical expression of this vasculitis. Visual ischemic manifestations, in particular permanent visual loss, constitute the most feared complications of GCA. Management of GCA is still fraught with challenges. Corticosteroids are the cornerstone of the therapy in GCA. The use of corticosteroids has dramatically reduced the frequency of blindness in patients with GCA. The initial dose of prednisone or its equivalent ranges between 40-60 mg/day, either as single or divided dose for 3-4 weeks. GCA patients without severe ischemic complications experience rapid disease improvement with an initial prednisone dose of 40 mg/day. In GCA patients who present with severe visual ischemic complication, higher steroid doses, generally an initial dose of 60 mg/prednisone/day or methylprednisolone pulse therapy (1g daily for 3 consecutive days) are given. There are data showing that not the absolute dose but the time from the onset of symptoms to the first administration of corticosteroids is predictive of improvement of visual loss. Regrettably, in most cases once established the prognosis for significant visual recovery in GCA patients despite corticosteroid therapy is poor. Immunosuppressive agents may be considered for use in large vessel vasculitis as adjunctive therapy. In this regard, alternative-corticosteroid sparing drugs, in particular methotrexate (MTX), should be considered in GCA patients with severe corticosteroid-related side effects and/or in those patients who require prolonged corticosteroid therapy due to relapses of the disease. However, a meta-analysis of randomized placebo-controlled trials in patients with newly diagnosed GCA disclosed modest efficacy of MTX (10-15 mg/week) in reducing relapse rate and lowering the cumulative corticosteroid therapy. No clear benefit of anti-TNF-alpha therapy in patients with GCA has been reported. Nevertheless, recent reports indicate that anti-IL6 receptor monoclonal antibody therapy may an effective and relatively safe therapy in patients with inflammatory aortitis refractory to corticosteroids or to other biologic immunosuppressive drugs. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.6113
Monostotic Paget’s Disease of a Cervical Vertebra
Authors: Rupert A Schmid, Katja Schwenzer, Mayo Weiss, Clemens Rock, Franz-Josef Rink, Klaus Hahn, Stefan Dresel
Journal: Clinical Nuclear Medicine (2002)

SCHMID, RUPERT A. M.D.*; SCHWENZER, KATJA M.D., D.D.S.†; WEISS, MAYO M.D.*; ROCK, CLEMENS M.D.‡; RINK, FRANZ-JOSEF M.D.‡; HAHN, KLAUS M.D.*; DRESEL, STEFAN M.D.* Author Information
Abstract P4-04-04: Immunohistochemical analysis of Cancer Testis antigens and Topoisomerase 2-alpha expression in triple negative breast carcinomas: a retrospective study
Authors: A Juretic, I Mrklic, GC Spagnoli, Z Pogorelic, S Tomic
Journal: Cancer Research (2012)

Abstract Aim. The aim of this study is to assess the expression of cancer testis (C/T) antigens in the TNBC group, expression of topoisomerase 2-alpha (TOPO2A), basal-like (BL) immunophenotype defined by basal markers (CK5/6, CK14, and EGFR), BL morphology and conventional clinicopathologic factors as well as to demonstrate their prognostic relevance with respect to this group of tumours. Methods. The study includes 83 patients who underwent surgery between January 2003 and December 2009. Slides were stained immunohistochemically with CK5/6, CK14, EGFR, Ki-67, TOPO2A, MAGE-A1, MAGE-A10, NY-ESO and multi-MAGE. Survival-time and multivariate survival analyses were performed for the purpose of identifying prognostic markers for tumours with more aggressive behaviour. Results. Of the 83 triple negative tumours, 55 (66.3%) have BL immunophenotype and 40 (48.2%) BL morphology. MAGE-A1 show the most frequent expression (69.0%), followed by multi-MAGE (58.0%), NY-ESO (27.1%) and MAGE-A10 (13, 2%). MAGE-A10 expression is significantly correlated with tumour size (p = 0.026). The expressions of MAGE-A1, MAGE-A10 and multi-MAGE are significantly correlated with clinical stage (p = 0.024, p = 0.041, p = 0.031, respectively). A significant correlation has been found between the expressions of MAGE-A10 and NY-ESO and the expression of TOPO2A (p = 0.005, p = 0.013). There is no significant correlation between the expression of C/T antigens and disease-free survival (DFS) or overall survival (OS). Conclusions. High expression levels of MAGE and NY-ESO observed in TNBC are of potential clinical relevance, especially in the adjuvant setting of treatment, and may be of therapeutic value as a vaccine-based treatment in the TN group of breast tumours for which therapeutic options are limited. Key words: triple negative breast cancer, basal-like breast cancer, cancer-testis antigen, topoisomerase 2-alpha, prognosis, survival analysis. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P4-04-04.
Abstract 375: Fluorescence-guided surgery leads to improved resection of primary pancreatic cancer and prolonged survival
Authors: Cristina A. Metildi Raimo, Sharmeela Kaushal, Chanae R. Hardamon, Cynthia S. Snyder, Robert M. Hoffman, Michael Bouvet
Journal: Cancer Research (2012)

Abstract The aim of this study was to determine if fluorescence-guided surgery (FGS) could improve surgical outcomes, reduce recurrence rates and improve overall survival in orthotopic mouse models of human pancreatic cancer. Orthotopic mouse models of human pancreatic cancer were established using the BxPC-3 RFP-expressing pancreatic cancer cell line in nude mice. Two weeks after implantation, the mice were randomized to undergo bright light surgery (BLS) or fluorescence-guided surgery (FGS). Pre- and postoperative images were obtained with the Olympus OV-100 Small Animal Imaging System to assess completeness of surgical resection. Whole body imaging of the mice was performed weekly in the postoperative period to assess for recurrence and follow tumor progression. Additionally, half of the mice were randomly selected to undergo 4 weeks of postoperative gemcitabine treatment. At six weeks postoperatively, or when premorbid, the mice were sacrificed and primary pancreatic tumor burden was measured using ImageJ v1.440. A more complete resection of pancreatic cancer was achieved using FGS compared to BLS: 98.9% vs 77.1%, p=0.005. Sixty-three percent of the mice undergoing BLS had evidence of gross residual disease, whereas 20% of mice undergoing FGS received a complete resection and an additional 75% of the mice were left with minimal residual disease (p=0.0001). At six weeks, the primary pancreatic tumor burden was significantly less with FGS compared to BLS: 19.341 ± 5.26 mm2 vs. 6.194 ± 3.61 mm2, p=0.048. In a pilot survival study, FGS alone was associated with a decreased risk of tumor recurrence (HR=0.093, 95% CI 0.013-0.645), and mean overall survival was lengthened from 18.5 weeks to 28.2 weeks. The addition of adjuvant gemcitabine (GEM) to FGS further lengthened mean overall survival to 42.75 weeks (p=0.019). Three of the four mice in the FGS with adjuvant gemcitabine group were alive at 12 months postoperatively and all of the mice (n=4) were free of tumor at time of sacrifice. In this study, we achieved improved surgical outcomes in primary pancreatic cancer under fluorescence-guidance. FGS afforded a more complete resection of primary tumor and improved disease-free survival and overall survival. The combination of FGS and GEM lengthened disease-free survival and improved overall survival. This novel approach has potential to improve outcomes in the surgical treatment of pancreatic cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 375. doi:1538-7445.AM2012-375
P07.02 * THE ROLE OF INTRAOPERATIVE NEUROPHYSIOLOGICAL MONITORING IN SURGERY OF INTRADURAL EXTRAMEDULLARY SPINAL CORD TUMORS
Authors: A. Scibilia, V. Rizzo, C. Terranova, A. Quartarone, A. Germano
Journal: Neuro-Oncology (2014)

INTRODUCTION: Surgery of intradural extramedullary spinal cord tumors (IESCT) carries the risk of new neurological deficits in the post-operative period. Intraoperative neurophysiological monitoring (IONM) represents the most effective technique able to improve clinical and functional outcomes, which are obtained through identification and real-time intraoperative monitoring of spinal cord, giving informations about neurological conduction of both, spinal cord long pathways and cauda equina radices. The most widely used and reliable techniques are somatosensory evoked potentials (SEPs), motor evoked potentials (MEPs) and D-wave registration. Although in the literature there are recent evidences of the IONM usefulness, there are not uniform opinions about the different techniques employed, and, their use in IESCT. In the present study we describe the results in our 2-year experience at the Neurosurgery of the University of Messina Hospital. MATERIALS AND METHODS: Our protocol includes evaluation of SEPs from posterior tibial nerve stimulation, MEPs obtained with elevate voltage electrical transcranial stimulation (through multiple myomeric registration of motor evoked responses from inferior limbs), D-Wave registration (through epi- and intradural registration after single pulse electrical transcranial stimulation) in spinal tumor surgery. RESULTS: We did not recorded any intraoperative complication by the use of our IONM protocol. In all patients IONM was useful for correct neurological and functional identification of spinal cord. We observed an optimal correlation between neurological examination on admission and responses from IONM of inferior limbs SEPs and MEPs. In fifty percent of patients, the D-wave registration was the most useful intraoperative tool, due to the lack of motor and somatosensory evoked responses. We matched all pre and post-operative neurological and neurophysiological data, along with tumors' features (localization, dimensions and histology) in order to quantify the impact of IONM on outcome. In our series, none of the patients presented with neurological worsening in the post-operative period. Conversely, surgical treatment of IESCT lead to a significant improvement of neurological status of our patients. CONCLUSIONS: Our preliminary data, although based on a limited series of patients evaluated at a single institution, confirm the role of IONM as an essential tool in operative work-up of all spine surgeries, including IESCT, to achieve an optimal post-operative functional outcome. This study gives further evidences about relevance, impact and usefulness of IONM in IESCT surgery.
Delay in Seeking Care for Cancer Symptoms: A Population-Based Study of Elderly New Mexicans
Authors: J. M. Samet, W. C. Hunt, M. L. Lerchen, J. S. Goodwin
Journal: JNCI Journal of the National Cancer Institute (1988)

To characterize the delay by the elderly in seeking care for cancer symptoms, we interviewed 800 New Mexicans, =65 years of age, with newly diagnosed cancer. Overall, 29.4% of the subjects were asymptomatic when cancer was detected, and 48.0% presented within 2 months of symptom onset. However, 19.2% of the subjects delayed seeking care for at least 12 weeks and 7.4% delayed at least 1 year. Site of cancer was the strongest determinant of delay. Hispanics tended to report longer delay than non-Hispanics, and age was not associated with delay. Of the numerous other factors considered, only having a regular checkup was significantly associated with delay interval. [J Natl Cancer Inst 1988; 80: 432–438]
Human Lymphoblastoid Cell Lines. I. Culture Methods and Examination for Epstein-Barr Virus
Authors: C. M. Steel, E. Edmond
Journal: JNCI Journal of the National Cancer Institute (1971)

A simple technique is described for the establishment of Iymphoblastoid cell lines from small samples of peripheral blood. Thirteen cell lines were grown from patients with infectious mononucleosis, acute and chronic leukemia, and myelofibrosis but none from healthy subjects. Indirect immunofluorescence and electron microscopy proved equally sensitive for the detection of Epstein-Barr virus (EBV) which was found, on initial examination, in 6 of 8 cell lines from patients with infectious mononucleosis but in 0 of 4 cell lines from patients with leukemia and myelofibrosis. Virus later appeared in 3 lines initially considered negative, probably due to the presence of a previously occult infection. Unusually high titers of EBV antibody were found in plasma from 5 of 10 adult patients with leukemia. Data from 3 children with acute leukemia suggested that in vivo lymphoproliferation is not the critical factor in determining whether a lymphoblastoid cell line will grow in vitro.
Recurrent Abdominal Pain of Childhood Due to Lactose Intolerance
Authors: Ronald G. Barr, Melvin D. Levine, John B. Watkins
Journal: New England Journal of Medicine (1979)

The role of lactose malabsorption was studied prospectively in 80 schoolchildren with recurrent abdominal pain. Malabsorption was documented in 40 per cent (16 of 59 whites, 12 of 16 blacks and four of five Hispanic children) on the basis of elevated levels of hydrogen in their breath. Those with lactose malabsorption, however, were not clinically distinguishable on the basis of past milk ingestion (P greater than 0.05), weekly pain frequency (median, five vs. six times), presence of diarrhea (40 vs. 27 per cent) or symptom response to lactose load. In children with malabsorption who completed a six-week diet trial, 70 per cent reported increased frequency of pain (P less than 0.002) when placed on their usual lactose-containing diet. Lactose malabsorption has a substantial role in the symptoms of children with recurrent abdominal pain, and it should be considered before performing invasive procedures or assuming a psychogenic origin.
UTILIZATION OF AN ADVANCED ABLATION LESION ANNOTATION SYSTEM WITH FORCE SENSING CATHETERS DRAMATICALLY REDUCES ADENOSINE INDUCED PULMONARY VEIN RECONNECTION FOLLOWING RADIOFREQUENCY ABLATION FOR ATRIAL FIBRILLATION
Authors: Guy Rozen, Kevin Heist, Moshe Rav Acha, Leon Ptaszek, Jeremy Ruskin, Moussa Mansour
Journal: Journal of the American College of Cardiology (2016)