tbuckley/cabot-search huggingface dataset:
Glycoproteomic Approach Identifies KRAS as a Positive Regulator of CREG1 in Non-small Cell Lung Cancer Cells
Authors: David J. Clark, Yuping Mei, Shisheng Sun, Hui Zhang, Austin J. Yang, Li Mao
Journal: Theranostics (2015)

Protein glycosylation plays a fundamental role in a multitude of biological processes, and the associated aberrant expression of glycoproteins in cancer has made them attractive biomarkers and therapeutic targets. In this study, we examined differentially expressed glycoproteins in cell lines derived from three different states of lung tumorigenesis: an immortalized bronchial epithelial cell (HBE) line, a non-small cell lung cancer (NSCLC) cell line harboring a Kirsten rat sarcoma viral oncogene homolog (KRAS) activation mutation and a NSCLC cell line harboring an epidermal growth factor receptor (EGFR) activation deletion. Using a Triple SILAC proteomic quantification strategy paired with hydrazide chemistry N-linked glycopeptide enrichment, we quantified 118 glycopeptides in the three cell lines derived from 82 glycoproteins. Proteomic profiling revealed 27 glycopeptides overexpressed in both NSCLC cell lines, 6 glycopeptides overexpressed only in the EGFR mutant cells and 19 glycopeptides overexpressed only in the KRAS mutant cells. Further investigation of a panel of NSCLC cell lines found that Cellular repressor of E1A-stimulated genes (CREG1) overexpression was closely correlated with KRAS mutation status in NSCLC cells and could be down-regulated by inhibition of KRAS expression. Our results indicate that CREG1 is a down-stream effector of KRAS in a sub-type of NSCLC cells and a novel candidate biomarker or therapeutic target for KRAS mutant NSCLC.
Use of a self-report symptom scale to detect depression in a community sample
Authors: J K Myers, M M Weissman
Journal: American Journal of Psychiatry (1980)

The authors gave the CES-D, a self-report depression symptom scale, to 515 people drawn from a longitudinal community survey. The subjects were also interviewed using the Schedule for Affective Disorders and Schizophrenia (SADS). From the information collected on the SADS, the subjects were given diagnoses based on Research Diagnostic Criteria. The results indicate a modest relationship between self-reported symptoms of depression and the diagnosis of a major or minor depression. However, the groups defined as "cases" by such reports also include many people with other diagnoses or with no diagnoses at all. Thus, symptom scales are useful for the screening of depressed persons in research studies but are only rough indicators of clinical depression in the community.
Abstract POSTER-BIOL-1309: Beta-catenin regulated ALDH1A1 is a target in ovarian cancer stem cells
Authors: Salvatore Condello, Sarbajeet Nagdas, Cynthia Morgan, John Turek, Thomas Hurley, Daniela Matei
Journal: Clinical Cancer Research (2015)

Abstract Spheroids are 3D multi-cell aggregates formed in non-adherent culture conditions. In ovarian cancer (OC), they serve as a vehicle for cancer cell dissemination in the peritoneal cavity and protect cells from extracellular stress-induced anoikis. We hypothesized that spheroids are enriched in cancer stem cells and investigated pathways activated under these conditions. Affymetrix-based gene expression profiling of OC cells grown as spheroids vs. monolayers identified β-catenin pathway being upregulated in 3D cultures. Increased expression of β-catenin and target genes (cyclin D1, c-myc) was demonstrated in spheroids vs. monolayers and in successive spheroid generations using OC cell lines and primary cultures. B-catenin function measured as TCF/LEF1 reporter activity was augmented in spheroids vs. monolayers and siRNA mediated β-catenin knock down decreased the number of spheroids (p&amp;lt;0.001). Along with β-catenin, the expression level of ALDH1A1, an OC stem cell marker, was increased in successive spheroid generations. The percentage of ALDH+ cells was significantly higher in spheroids vs. monolayers in IGROV1, A2780, SKOV3, and primary OC cells. B-catenin knock-down decreased ALDH1A1 expression, suggesting that it is a β-catenin target. The percentage of ALDH+ cells and the number of spheroids formed were increased in the cell population dissociated from OC xenografts treated with carboplatin compared to control (44% vs. 2.3%), supporting that ALDH1A1 is a stem cell marker. A37 is a novel ALDH1A1 enzymatic inhibitor (Ki of 5nM). A37 decreased cell viability and spheroid formation by IGROV1 cells (p&amp;lt;0.001). A37 also decreased the percentage of ALDH+ cells under 3D culture conditions. These data support the role of β-catenin regulated ALDH1A1 in the maintenance of OC spheroids and of a stem cell phenotype and propose new ALDH1A1 inhibitors targeting this cell population. (Funding: US Department of Veterans Affairs and Ovarian Cancer Research Fund) Citation Format: Salvatore Condello, Sarbajeet Nagdas, Cynthia Morgan, John Turek, Thomas Hurley, Daniela Matei. Beta-catenin regulated ALDH1A1 is a target in ovarian cancer stem cells [abstract]. In: Proceedings of the 10th Biennial Ovarian Cancer Research Symposium; Sep 8-9, 2014; Seattle, WA. Philadelphia (PA): AACR; Clin Cancer Res 2015;21(16 Suppl):Abstract nr POSTER-BIOL-1309.
PTH-206 Audit on appropriateness intravenous fluid therapy in tpn patients
Authors: M Toqeer, A Stotesbury, D Schneider, L Timbers, J Whittome, J Jegard, A Jenkins
Journal: Gut (2015)

<h3>Introduction</h3> In hospitalised patients, fluid resuscitation is the second commonest intervention after oxygen administration. The situation becomes complex when fluids are prescribed to patients on parenteral nutrition (TPN), as TPN contributes to their fluid requirements. Excess IV fluids can cause complications such as fluid overload in sick patients with multiple co-morbidities. <h3>Method</h3> The aim of the study was to assess the appropriateness of additional IV fluid therapy in patients receiving TPN at our hospital. We prospectively reviewed patients receiving TPN for 3 months from October to December 2014. We used dietitian’s charts, medical notes and drug charts to gather data, which included indications, amount and type of additional fluids given and fluid balance. We also noted whether there were any complications such as fluid overload. The data included patient gender, age and whether a medical or surgical team looked after them. Additional fluids were described as appropriate if given for renal impairment or to match the fluid balance e.g. insensible or extra losses. <h3>Results</h3> A total of thirty patients were given TPN during the three months. Age range was 35–90 with an average age of 58. There were equal number of male and female patients. 22 (73%) patients were managed on surgical wards, the remainder on medical wards. 26 (86%) patients were prescribed additional IV fluids during their admission. Indications for fluids were not documented in 24/26 (92%). 15 patients were in a positive fluid balance of at least 2 litres over an average duration of 3 days. Of these, 10 patients had renal impairment, which subsequently improved. 4 patients had IV fluids prescribed to compensate for fluid losses e.g. from a high stoma output. 14/26 (53%) fluid prescriptions were appropriate. The choice of fluids was variable as most patients had a mixture of fluids, approximately 50% given at least one litre dextrose as fluids. All of the fluid prescriptions were made by the house officers. Two patients developed fluid overload requiring diuretics. There were 2 deaths unrelated to fluid management but secondary to the underlying conditions. <h3>Conclusion</h3> The results demonstrate that additional IV fluid prescription was often poorly managed. The indication for fluids was not documented in most cases and the choice and volume of fluids were often inappropriate. This is particularly relevant in TPN patients as more dextrose can increase the caloric burden. We propose that a multidisciplinary nutrition team should take the lead in advising about fluid management as well as TPN and only experienced practitioners should prescribe fluids in these scenarios. Juniors prescribing additional fluids should be supervised or advised. <h3>Disclosure of interest</h3> None Declared.
Peroral endoscopic myotomy for esophageal muscular ring
Authors: Ga Song, Weon Ko, Won Kim, Ki Hahm, Sung Hong, Joo Cho
Journal: Endoscopy (2015)

Lower esophageal muscular ring, usually called A-ring, is a rare esophageal lesion that occasionally causes dysphagia or symptoms of reflux [1] [2]. There have been only a few case reports of attempts to treat muscular ring with the intramuscular injection of botulinum toxin (botox) [1] [3]. This report is believed to be the first of the use of peroral endoscopic myotomy (POEM) to treat esophageal muscular ring.
Lumpectomy and radiation: improving the outcome.
Authors: A S Lichter
Journal: Journal of Clinical Oncology (1992)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1992.10.3.349 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 1740676 Lumpectomy and radiation: improving the outcome. A S LichterxA S LichterSearch for articles by this author https://doi.org/10.1200/JCO.1992.10.3.349 First Page PDF © 1992 by American Society of Clinical Oncology
Drainage of Deep Pelvic Abscesses Using Therapeutic Echo Endoscopy
Authors: M Giovannini, E Bories, V Moutardier, C Pesenti, A Guillemin, B Lelong, J R Delpéro
Journal: Endoscopy (2003)

The purpose of this study was to evaluate the clinical efficacy of endosonographically guided transrectal aspiration and drainage by plastic stent of deep pelvic abscesses, using a therapeutic echo endoscope device.Between September 2000 and June 2001, 12 patients (nine men, three women, mean age 67 years) were treated for a perirectal or a pelvic abscess using an endoscopic ultrasound (EUS) technique. The drainage of these fluid collections was performed under EUS guidance, using therapeutic EUS scopes with a large working channel.No major complication occurred during this study. Transrectal stent insertion succeeded in nine patients. In three patients, only aspiration was possible. Among the nine patients in whom a stent was successfully introduced into the fluid collection, complete drainage without relapse was achieved in eight patients (mean follow-up 10.6 months, range 6-14 months). The stent was removed endoscopically after 3 to 6 months. Drainage was incomplete in one patient (with a large abscess, diameter > 8 cm), who subsequently underwent surgical drainage. However, two out of the three patients in whom aspiration alone was performed developed a recurrence of the abscess and required surgical treatment.EUS-guided drainage of deep pelvic abscesses could offer an alternative treatment to surgery in the management of these postoperative complications.
PTH-042 Spiral snare resection and hybrid endoscopic mucosal ablation: a comparison of outcomes after piecemeal resection/ablation
Authors: ZP Tsiamoulos, R Rameshshanker, N Suzuki, L Bourikas, BP Saunders
Journal: Gut (2015)

<h3>Introduction</h3> Incomplete endoscopic removal of colorectal polyps causes severe submucosal fibrosis, making subsequent endoscopic resection challenging. Two approaches to removal of recurrent polyp over a scarred submucosa or to polyps with inherent submucosal fibrosis such as NG-LST’s are either to use a stiff braided snare which helps capture tissue or to firstly snare as much tissue as possible using a conventional snare and then destroy tissue over the centre of the scar with high power APC preceded by submucosal injection (Endoscopic Mucosal Ablation, EMA). <h3>Method</h3> A prospective database (Jan 2010–Aug 2012) was used to identify large (&gt;2 cm) colorectal polyps removed in a piecemeal fashion using either hybrid EMR/EMA technique or spiral snare EMR (SS-EMR, Olympus, Keymed, UK) at our tertiary referral centre. Patient/polyp/technique-related details and short/long term endoscopic surveillance data were retrieved and analysed (chi-square/Fischer’s). The aim of this study was to compare the safety, technical success and recurrence between each group (group A – hybrid EMR/EMA, group B – SS-EMR). <h3>Results</h3> This study enrolled 56 patients in group A and 48 patients in group B. Median polyp size was 3 cm (range 2–8 cm) for group A and 5 cm (2–15 cm) for group B. The majority (64.28%) of polyps in group A had previous failed polypectomy attempts compared to only 27% of polyps in group B. There was no difference in time taken to complete the procedure between the groups (mean time for group A – 36 min vs group B – 39 min). Procedural bleeding (A vs B were 7.1% vs 18.8% p = 0.13) and delayed bleeding (A vs B were 19.6% vs 37.5%, p = 0.05) were more common in group B. There were no peforations in either group. Two (4.2%) patients in SS-EMR group developed post polypectomy syndrome and needed brief hospital admission. Four (7.1%) patients in hybrid EMR/EMA cohort developed delayed bleeding requiring admission for transfusion; all made an uneventful recovery. There was no significant difference in polyp recurrence at 24 months between the groups (A vs B 12.5% vs 8.3%, p = 0.44). No patients have required surgery to date. <h3>Conclusion</h3> Spiral snare resection and hybrid mucosal resection/ablation modalities are both safe and feasible to eradicate recurrent fibrotic colorectal polyps. Delayed bleeding overall is significantly lower when using the hybrid resection/ablation technique though may be more severe when it does occur. Medium term outcomes appear similar in both groups with acceptable levels of endoscopically manageable recurrence. <h3>Disclosure of interest</h3> None Declared.
Mycosis Fungoides
Authors: Robert C. Young
Journal: New England Journal of Medicine (1989)

Mycosis fungoides is one of a family of uncommon cutaneous T-cell lymphomas that includes the Sézary syndrome, tumeur d'emblée, Ki-1–positive lymphoma, and the adult T-cell leukemia or lymphoma caused by the type C retrovirus human T-cell lymphotropic virus Type I. All but mycosis fungoides and the Sézary syndrome can be distinguished by their aggressive presentation, early visceral spread, poor prognosis, unique histologic features, and lymphocytic or retroviral markers.1 Mycosis fungoides and its variant, Sézary syndrome, in which circulating cells are associated with erythroderma, are relatively indolent lymphomas composed of thymus-derived lymphocytes. They have a variable natural history and may remain . . .
Phototheranostic Porphyrin Nanoparticles Enable Visualization and Targeted Treatment of Head and Neck Cancer in Clinically Relevant Models
Authors: Nidal Muhanna, Cheng S Jin, Elizabeth Huynh, Harley Chan, Yi Qiu, Wenlei Jiang, Liyang Cui, Laura Burgess, Margarete K Akens, Juan Chen, Jonathan C Irish, Gang Zheng
Journal: Theranostics (2015)

Head and neck cancer is the fifth most common type of cancer worldwide and remains challenging for effective treatment due to the proximity to critical anatomical structures in the head and neck region, which increases the probability of toxicity from surgery and radiotherapy, and therefore emphasizes the importance of maximizing the targeted ablation.We have assessed the effectiveness of porphysome nanoparticles to enhance fluorescence and photoacoustic imaging of head and neck tumors in rabbit and hamster models.In addition, we evaluated the effectiveness of this agent for localized photothermal ablative therapy of head and neck tumors.We have demonstrated that porphysomes not only enabled fluorescence and photoacoustic imaging of buccal and tongue carcinomas, but also allowed for complete targeted ablation of these tumors.The supremacy of porphysome-enabled photothermal therapy over surgery to completely eradicate primary tumors and metastatic regional lymph node while sparing the adjacent critical structures' function has been demonstrated for the first time.This study represents a novel breakthrough that has the potential to revolutionize our approach to tumor diagnosis and treatment in head and neck cancer and beyond.
Study of 60 patients with AIDS or AIDS-related complex requiring psychiatric hospitalization
Authors: J W Baer
Journal: American Journal of Psychiatry (1989)

Sixty patients with acquired immune deficiency syndrome (AIDS) or AIDS-related complex were treated on a locked inpatient psychiatric unit over a 30-month period. The wide range of mental disorders exhibited by these patients, key aspects of patient management, and the difficulty in psychiatric differential diagnosis are described.
The Effect of Guanazolo on Antibody Formation
Authors: R. A. Malmgren, B. P. Bennison, T. W. McKinley
Journal: JNCI Journal of the National Cancer Institute (1952)

The Effect of Guanazolo on Antibody Formation R. A. Malmgren, R. A. Malmgren National Cancer Institute3Bethesda, Md. Search for other works by this author on: Oxford Academic PubMed Google Scholar B. P. Bennison, B. P. Bennison National Cancer Institute3Bethesda, Md. Search for other works by this author on: Oxford Academic PubMed Google Scholar T. W. McKinley T. W. McKinley National Cancer Institute3Bethesda, Md. Search for other works by this author on: Oxford Academic PubMed Google Scholar 2Present address: Division of Chronic Diseases and Tuberculosis, Bureau of State Services, Public Health Service, Federal Security Agency, Washington 25, D. C. 3National Institutes of Health, Public Health Service, Federal Security Agency. Author Notes JNCI: Journal of the National Cancer Institute, Volume 12, Issue 4, February 1952, Pages 807–818, https://doi.org/10.1093/jnci/12.4.807 Published: 01 February 1952 Article history Received: 04 October 1951 Published: 01 February 1952
Case 32-1989
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, William F. McNeely, Betty U. McNeely, David M. Systrom, Eugene J. Mark
Journal: New England Journal of Medicine (1989)

Presentation of CaseA 61-year-old man was admitted to the hospital because of a recurrent lung abscess in the left lower lobe.He was well until six months earlier, when a cough and fever developed four to six weeks after he was treated for a dental abscess, with capping of two molar teeth. An x-ray film of the chest, obtained at another hospital, was reported to show an abscess in the left lower lobe. Bronchoscopic examination revealed severe ulcerative bronchitis in the superior segment of the left lower lobe; no endobronchial lesions were observed. Microscopical examination of specimens of sputum . . .
Endosonographic Misdiagnosis of Tumor Recurrence after Surgery for Malignant GIST
Authors: H. Kimura, Y. Shima, S. Kinoshita, I. Takahashi, T. Okai
Journal: Endoscopy (2002)

Submucosal tumors (SMTs) in the digestive tract include neoplastic and non-neoplastic lesions. Attempts have been made to grade the severity of mesenchymal tumors pathologically, using various criteria for malignancy, such as tumor size, cellularity, mitotic index, and DNA content [1]. We recently treated a patient with a malignant gastrointestinal stromal tumor (GIST), and describe an interesting diagnostic pitfall which arose from the use of endoscopic ultrasonography for follow-up examination after the operation.
Toward a clinical model of psychotherapy for depression, II: An integrative and selective treatment approach
Authors: T B Karasu
Journal: American Journal of Psychiatry (1990)

This is the second in a two-part series on psychotherapy for depression. Considering depression as a spectrum phenomenon, the author goes beyond manual-based diagnosis to suggest criteria for the use of psychodynamic, cognitive, or interpersonal psychotherapy, with or without pharmacotherapy. He proposes a clinical model that is both integrative and selective and recommends shifting and sharing different therapeutic perspectives in tailoring treatment to the depressed individual.
The Chemotherapeutic Selection for Pancreatic Cancer Patients with Malignant Ascites
Authors: Ryoji Takada, Tatsuya Ioka, Hironari Sueyoshi, Takuo Yamai, Nobuyasu Fukutake, Nobuko Ishida, Reiko Ashida, Kazuhiro Katayama
Journal: Annals of Oncology (2014)
FRI0603 Synovial Fluid Analysis and Crystal Detection: Results from a Cohort of Possible Artropathic Consecutive Patients with Effusion
Authors: P. Montagna, R. Brizzolara, A.C. Trombetta, S. Soldano, C. Ferrone, M. Cutolo, M.A. Cimmino
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> Among rheumatic diseases, gout and pseudogout result from the presence of specific crystals in the synovial fluid (SF) of inflamed joints, monosodium urate (MSU) and calcium pyrophosphate dehydrate (CPPD) crystals, respectively. Crystal detection and identification by a compensated polarized microscope allows a quick and definitive diagnosis of both gout and CPPD arthritis, and it is the accepted gold standard (1,2). SF examination allows to identify a variety of other crystals and particles that can induce arthropathy (3). <h3>Objectives</h3> To study a cohort of patients with SF effusion, according to specific crystal types detected. <h3>Methods</h3> Arthrocentesis was performed in 589 patients for cytological evaluation and crystal detection. For crystal detection, SF was examined by a compensated polarized microscope (400x), initially under ordinary light, subsequently under simple polarized light, finally adding a first-order red compensator to allow crystal identification by their birefrangence (2). SFs were divided into 5 groups, according to crystal type found: MSU, CPPD, apatite, aspecific crystals and without crystals. According to leukocyte count, SFs were divided into non-inflammatory (leukocytes &lt;2000/μl), moderately inflammatory (between 2000/μl and 5000/μl), inflammatory (between 5000/μl and 50000/μl) and highly inflammatory or infectious (&gt;50000/μl). Statistical analysis was performed by χ<sup>2</sup> and Mann-Whitney tests. <h3>Results</h3> Of the 589 patients considered, 298 (50,6%) were males and 291 (49,4%) females, mean age was 69.18±17.3 yrs. Aspirated joints were: knee (487 patients), elbow (29 patients), shoulder (28 patients), 1 MTP joint (23 patients), ankle (4 patients), hand (9 patients) and wrist (9 patients). Of note, the subdivision in groups by leukocyte count showed a significant association between the presence of MSU crystals and 5000-50000/ml counts (p=0.03) (Table). The increase in the number of leukocytes significantly correlates with the presence of CPPD crystals (p=0.01) (Figure). A significant correlation was also observed between leukocyte count &lt;2000/ml and the different groups (MSU, apatite, aspecific crystals and no crystals: p=0.02, p=0.006, p=0.02, p=0.02 respectively) (Table). <h3>Conclusions</h3> This study confirms that a high leukocyte count in SF suggests the presence of gout and pseudogout, according to literature data (4). Moreover, SF analysis was confirmed as important also in patients whit a mild joint inflammation. <h3>References</h3> Reginato A et al. Clin Geriatr Med 1988;4:295. 2. Pascual E et al. Cur Opin Rheumatol 2011;23:161-69. 3. Pascual E et al. Ann Rheum Dis 2007;66:1056-58. 4. Dougados M. Bailliere9s Clin Rheumatol 1996;10:519-34. <h3>Disclosure of Interest</h3> None declared