tbuckley/cabot-search huggingface dataset:
Two novel type 2N von Willebrand disease–causing mutations that result in defective factor VIII binding, multimerization, and secretion of von Willebrand factor
Authors: Simon Allen, Adel M. Abuzenadah, Joanna L. Blagg, Joanna Hinks, I. Mandy Nesbitt, Anne C. Goodeve, Turkiz Gursel, Jørgen Ingerslev, Ian R. Peake, Martina E. Daly
Journal: Blood (2000)
GP practices rated “outstanding” are better funded, BMA research finds
Authors: Ingrid Torjesen
Journal: BMJ (2016)

General practices rated as “outstanding” or “good” by England’s Care Quality Commission receive more funding per patient than those rated “inadequate” or “needs improvement,” research by the BMA indicates.1

The research looked at 2814 general practices rated by the CQC in 2015, cross matching their ratings against their funding. The results showed that practices rated as “outstanding” or “good” by …
&lt;emph type="ital"&gt;Calm Morning&lt;/emph&gt;
Authors: Janet M. Torpy
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract A28: Osteopontin splicing isoforms expression is modulated by partial epithelial mesenchymal transition in ovarian carcinoma cells.
Authors: Ingridy Celestino, Isabella S. Guimarães, Leticia B. A. Rangel, Etel R. P. Gimba
Journal: Clinical Cancer Research (2016)

Abstract In ovarian carcinoma (OC), invasive cell properties are supported by epithelial mesenchymal transition (EMT). We previously demonstrated that OPNc, one of the glycophosphoprotein osteopontin splicing isoforms (OPN-SI), activates OC progression. However , the involvement of these OPN-SI in EMT in OC cells has not previously been addressed. We aimed to evaluate the expression of OPN-SI in OC cells induced to EMT by TGFβ. ES2 and A2780 OC cell lines were treated with 10ng/ml of TGFβ in order to induce EMT. The expression of OPN-SI and EMT epithelial (E) (Claudin-3, Claudin-4 and E-cadherin) and mesenchymal (M) (slug, vimentin and N-cadherin) markers was tested by quantitative real time PCR. In both cell lines, OPNa is overexpressed in relation to OPNb and OPNc. Upon treatment with TGFβ, in A2780 cells we observed an upregulation of OPNc. Conversely, OPNa and OPNb were downregulated. In ES2 cells, TGFβ also induced OPNc upregulation, but OPNb expression is activated as well, while OPNa expression is inhibited. In order to validate EMT induction by TGFβ, we tested the expression of E and M markers. In A2780 cells, TGFβ induce the downregulation of E-cadherin, but Claudin-3 and Claudin-4 are upregulated. In ES2 cells, Claudin-3 expression is downregulated in this condition. After TGFβ treatment, in A2780 cells all M markers are downregulated, while in ES2 cells, only N-cadherin expression has been inhibited. Our data indicate that the expression of OPN-SI is modulated by EMT induced by TGFβ and that mostly the oncogenic OPNc is upregulated in this condition. Our data evidence that the expression of OPN-SI is modulated by a partial EMT phenotype induced by TGFβ, which is cell-type specific, and that OPN-SI, specially OPNc, could contribute to this key step on tumor progression in OC cells. Citation Format: Ingridy Celestino, Isabella S. Guimarães, Leticia B. A. Rangel, Etel R. P. Gimba. Osteopontin splicing isoforms expression is modulated by partial epithelial mesenchymal transition in ovarian carcinoma cells. [abstract]. In: Proceedings of the AACR Special Conference on Advances in Ovarian Cancer Research: Exploiting Vulnerabilities; Oct 17-20, 2015; Orlando, FL. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(2 Suppl):Abstract nr A28.
BCR–ABL-specific CD4+ T-helper cells promote the priming of antigen-specific cytotoxic T cells via dendritic cells
Authors: Norihiro Ueda, Rong Zhang, Minako Tatsumi, Tian-Yi Liu, Shuichi Kitayama, Yutaka Yasui, Shiori Sugai, Tatsuaki Iwama, Satoru Senju, Seiji Okada, Tetsuya Nakatsura, Kiyotaka Kuzushima, Hitoshi Kiyoi, Tomoki Naoe, Shin Kaneko, Yasushi Uemura
Journal: Cellular and Molecular Immunology (2016)
Saphenous vein bypass for coronary artery disease.
Authors: M J Levy, Y Zepnik, B Vidhe, Y Agmon, M Wurtzel
Journal: BMJ (1972)

The prevalence of occlusive coronary artery disease con- tinues to increase.'Almost certainly it has a variety of causes, but they remain elusive.Some associations are recognized: a family history of the disease, high blood pressure, smoking, physical inactivity, diabetes, obesity, hyperlipidaemia, hyperuricaemia, and a high blood haemato- crit.Others have been suggested, including carbon mon- oxide contamination of urban air, soft drinking water, and diet with a low fibre content.Until the disease can be pre- vented its treatment will continue to stretch our resources,
631. Rankl Knock-Out Mesenchymal Stromal Cells Have an Unexpected Osteogenic Differentiation Defect Which Is Improved by a RANKL-Expressing Lentiviral Vector
Authors: Ciro Menale, Francesca Schena, Lorenzo Diomede, Lucia Sergi Sergi, Lucia Susani, Eleonora Palagano, Elisabetta Traggiai, Cristina Sobacchi, Anna Villa
Journal: Molecular Therapy (2016)

Osteoclast-poor RANKL-dependent Autosomal Recessive Osteopetrosis (ARO) is a rare bone disease characterized by an increase in bone density due to the failure of bone resorption by impaired osteoclast formation. Hematopoietic stem cell transplantation is not an effective therapy for this ARO form, since in bone RANKL is produced mainly by cells of mesenchymal origin. Therefore Mesenchymal Stromal Cells (MSC) transplantation together with a gene-therapy strategy to correct RANKL defect in MSC could represent a possible effective therapy. Of note, whether also MSC, besides the osteoclasts, are affected by RANKL deficiency is unknown. To verify this, we established and characterized bone marrow derived MSC (BM-MSC) lines from the Rankl−/− (KO) mouse model, which recapitulates the human disease, and from wild type (WT) mice. No differences were found between KO and WT MSC in terms of morphology, immunophenotype and proliferation capacity. However, KO MSC displayed a reduced clonogenic potential with a decrease in stemness genes expression. KO MSC were able to normally differentiate towards the adipogenic and chondrogenic lineages, while showed a significantly impaired osteogenic differentiation capacity compared to WT MSC, as demonstrated by reduced Alizarin Red staining (ARS) and expression of osteogenic genes. To confirm that this alteration was due to the lack of functional RANKL, we developed a third generation lentiviral vector expressing human soluble RANKL (hsRL) for the genetic correction of KO MSC. We first investigated lentiviral transduction in 293T cells to optimize transduction efficiency at different multiplicity of infection (MOI) ranging from 1 to 100. hsRL production increased proportionally to the MOI and was stable over time. However, the higher the MOI the higher the cytotoxicity observed. Based on these data, we performed a lentiviral hsRL transduction in KO MSC at 20 and 50 MOI, to define the optimal transduction conditions. After transduction 99.5% of MSC were GFP+. While in Rankl−/− control cells the cytokine was not detected, in corrected cells hsRL production and secretion was measurable and comparable to sRL levels in WT mouse. KO MSC stably expressing hsRL showed an improved osteogenic differentiation capacity compared to untransduced KO MSC, as demonstrated by increased ARS and expression of osteogenic genes. Moreover, the expression of RANK receptor in both MSC suggested an autocrine role of sRL as possible mechanism. Our data suggest that restoration of RANKL production in lentiviral-transduced KO MSC might not only allow osteoclast differentiation in Rankl−/− mice upon transplantation, but also improve the osteogenic differentiation defect of KO MSC.
Consistent amounts of acute leukemia-associated P190BCR/ABL transcripts are expressed by chronic myelogenous leukemia patients at diagnosis
Authors: G Saglio, F Pane, E Gottardi, F Frigeri, MR Buonaiuto, A Guerrasio, D de Micheli, A Parziale, MN Fornaci, G Martinelli, F Salvatore
Journal: Blood (1996)
Impact of a district branch Task Force on women
Authors: E Hilberman, M Gispert, J Harper
Journal: American Journal of Psychiatry (1976)

A questionnaire sent to all women psychiatrists and trainees in North Carolina as part of the development of a district branch Task Force on Women indicated that institutional sexism affects the careers of many of the respondents. Problems of family-professional role conflicts were especially pronounced in residents and faculty women. Survey findings have resulted in recommendations in the areas of education, communication, and increasing women's participation in task force and district branch activities. The authors feel that such task forces provide a much needed network of supportive relationships for women psychiatrists and raise the consciousness of the district branch regarding women's issues.
Fulfilling the Promise of “Anyone, Anywhere” to Perform CPR
Authors: Mickey S. Eisenberg, Bentley J. Bobrow, Tom Rea
Journal: JAMA (2014)

Closed-chest cardiac massage for the treatment of cardiac arrest was first described in the medical literature in 1960.
The report provided the basis to revolutionize care for one of the leading causes of death. In a statement filled with hope and promise, the authors wrote that “Anyone, anywhere, can now initiate cardiac resuscitative procedures. All that is needed are two hands.” Prior to closed-chest massage, the only recourse to achieve artificial circulation was open-chest massage involving emergency thoracotomy. Today the procedure widely known as cardiopulmonary resuscitation (CPR) has been shown to double or triple the odds of survival from cardiac arrest.2
The Incidence and Risk Factors For Hematological Malignancies and Premalignant Hematological Disorders In HIV
Authors: Erin Jou, Oleg Gligich, Alvita CY Chan, Diwakar Mohan, Felsen Uri, Henny Heisler Billett, Anthony T.C. Chan, Radha Raghupathy
Journal: Blood (2013)
Abstract 300: Pathophysiologically Regulated Gene Expression in Human Stem &amp; Progenitor Cells for Cardiogenic Differentiation and Repair
Authors: Zhilin Chen, Sean R Hall, Keith R Brunt, Zhan Liu, David A Ademidun, Stephen C Pang, Christopher A Ward, Luis G Melo
Journal: Circulation (2008)

Human stem and progenitor cells have emerged as potentially useful substrates for cardiovascular repair through neovascularization and myocardial regeneration. However, efficacy is limited by impedance to stem cell retention, homing and differentiation in hostile microenvironments, as occur in infarcted myocardium. The objective of the current study was to regulate gene function for tailored therapy in post infarct myocardium. Here we show that hypoxic and inflammatory stimuli of the infarct microenvironment regulate a proportional response in gene expression in human endothelial progenitor (EPC) and mesenchymal stem cells (MSC). Highly efficient lentiviral vectors incorporating hypoxia (HRE) and nuclear factor kappa B (NFkB) responsive elements are used to drive transgenes for survival, autologous stem cell homing and cardiogenic differentiation. Utilizing an internal cytomegalovirus promoter deleted lentiviral transfer vector, an HRE-NFkB bicistronic promoter-reporter vector was constructed with a modified internal ribosome entry sequence between green fluorescent protein and luciferase or therapeutic genes. Either hypoxia or inflammation resulted in a seven to ten-fold response of transgene expression assessed by luciferase activity in EPC (hypoxia, 7608±954; inflammation 11492±1384, P&lt;0.01 and P&lt;0.001 vs control 1049±139 respectively, N=6), while combined hypoxic-inflammatory stimuli resulted in a sixty-fold increase of transgene expression (hypoxic-inflammation, 62364±6609, P&lt;0.001 vs control 1049±139, N=6). These results were recapitulated in MSC and with a series of therapeutic genes as determined by transcript, protein expression and activity. Our results demonstrate that regulated vectors provide a proportional response to hostile post-infarct myocardium. Translating cardiovascular regenerative medicine using stem cells requires managing stem cell survival, function and differentiation. Utilizing site-specific pathophysiological cues to auto-regulate reparative and regenerative gene expression, this study is a starting point for sophisticated platforms for patient tailored cell-based cardiogenic therapy.
Menopause-related affective disorders: a justification for further study
Authors: P J Schmidt, D R Rubinow
Journal: American Journal of Psychiatry (1991)

Despite descriptions dating back to the nineteenth century of menopause-related affective syndromes, recent investigations have been unable to characterize a specific disturbance of mood or behavior related to this period of life. In this article the authors identify a number of methodologic problems in earlier studies and suggest that further study is warranted and that it is premature to conclude that menopause-related affective syndromes do not exist. They then provide guidelines for examining mood and behavioral changes that take place during the climacteric and menopause, with the hope of stimulating further research which may determine whether menopause-related affective disorders exist and, if so, their characteristics.
PAD Given at Relapse Is More Effective Than VAD Given as Induction Therapy - Results of a Phase II Study.
Authors: Treen C. Morris, Paul J. Kettle, Mary B. Drake, Aisleen Brunton, Gordon Cook, Maeve Leahy, Michael O’Dwyer, Helen Enright, Tanya O’Shea, Rakesh Popat, Heather Oakervee, James D. Cavenagh
Journal: Blood (2007)
bmjlearning.com: Blended learning
Authors: Kieran Walsh
Journal: BMJ (2005)

“What is `blended learning'? It's the use of two or more distinct methods of training. This may include combinations such as: blending classroom instruction with online instruction, blending online instruction with access to a coach or faculty member or blending simulations with structured courses.”

So said Elliot Masie, a leading authority on e-learning.1 Blended learning involves offering different options to learners. As most learners have different learning styles, they are then more likely to find something that suits them. But good blended learning should be more than just a simple “pick and mix” approach. The “pick and mix” approach involves looking at what learning resources you've got and sticking them together to make a course. Such courses often offer the same material in different formats (and, at their worst, different parts of the course contradict each other).

If you are thinking about setting up a course then you should think through these issues. What are the optimal blends to suit your learners? If you are running a course on cardiology for a group of registrars, then you should consider combining online learning content, so your students can learn knowledge; work based approaches for practical skills; and perhaps online discussion groups or face to face debates on ethical dilemmas, so that they can develop their feelings and attitudes. This is a good blend—you are using learning technology that is tailored to achieve the outcomes that you want. You have also concentrated on the “3 Rs” of medical education—knowledge, skills, and attitudes. A poor blend would be to teach practical skills in lectures and to advise students to develop communication skills from reading textbooks.

At BMJ Learning, we publish different types of resources according to the learning outcomes that our authors are trying to achieve. In “read, reflect, respond” modules we encourage you to read about a topic, think over the issues outlined, and give your opinions. One of our latest such modules is on discharge planning—for which there is rarely an absolutely right answer—and the reflective format allows author and readers to suggest possible solutions to some common dilemmas. For example, how would you plan the discharge of a 75 year old man who is severely disabled? You think he should go to a local nursing home, but his family insists that he would be better off with them. He is too confused to let you know what he would like. To find out what the author and other readers would do in this circumstance have a look at www.bmjlearning.com. You can also contribute your own solutions to the debate.
Economic Outcomes With Anatomical Versus Functional Diagnostic Testing for Coronary Artery Disease
Authors: Daniel B. Mark, Jerome J. Federspiel, Patricia A. Cowper, Kevin J. Anstrom, Udo Hoffmann, Manesh R. Patel, Linda Davidson-Ray, Melanie R. Daniels, Lawton S. Cooper, J. David Knight, Kerry L. Lee, Pamela S. Douglas
Journal: Annals of Internal Medicine (2016)

PROMISE (PROspective Multicenter Imaging Study for Evaluation of Chest Pain) found that initial use of at least 64-slice multidetector computed tomography angiography (CTA) versus functional diagnostic testing strategies did not improve clinical outcomes in stable symptomatic patients with suspected coronary artery disease (CAD) requiring noninvasive testing.To conduct an economic analysis for PROMISE (a major secondary aim of the study).Prospective economic study from the U.S. perspective. Comparisons were made according to the intention-to-treat principle, and CIs were calculated using bootstrap methods. (ClinicalTrials.gov: NCT01174550).190 U.S. centers.9649 U.S. patients enrolled in PROMISE between July 2010 and September 2013. Median follow-up was 25 months.Technical costs of the initial (outpatient) testing strategy were estimated from Premier Research Database data. Hospital-based costs were estimated using hospital bills and Medicare cost-charge ratios. Physician fees were taken from the Medicare Physician Fee Schedule. Costs were expressed in 2014 U.S. dollars, discounted at 3% annually, and estimated out to 3 years using inverse probability weighting methods.The mean initial testing costs were $174 for exercise electrocardiography; $404 for CTA; $501 to $514 for pharmacologic and exercise stress echocardiography, respectively; and $946 to $1132 for exercise and pharmacologic stress nuclear testing, respectively. Mean costs at 90 days were $2494 for the CTA strategy versus $2240 for the functional strategy (mean difference, $254 [95% CI, -$634 to $906]). The difference was associated with more revascularizations and catheterizations (4.25 per 100 patients) with CTA use. After 90 days, the mean cost difference between the groups out to 3 years remained small.Cost weights for test strategies were obtained from sources outside PROMISE.Computed tomography angiography and functional diagnostic testing strategies in patients with suspected CAD have similar costs through 3 years of follow-up.National Heart, Lung, and Blood Institute.
Abstract P1-12-08: Factors associated with delays in chemotherapy initiation among patients with breast cancer
Authors: K Losk, I Vaz Duarte Luis, K Camuso, M Lloyd, S Kadish, J Hirshfield-Bartek, L Cutone, M Golshan, N Lin, C Bunnell
Journal: Cancer Research (2016)

Abstract Background: National guidelines endorse time-dependent quality metrics for breast cancer care. We examined factors associated with delays in chemotherapy initiation at an NCI designated comprehensive cancer center. Methods: We identified 523 patients who received post-operative adjuvant chemotherapy between January 2011 and December 2013 at our center. We defined 28 days from last definitive surgery (LDS) to chemotherapy as the target timeframe, and unacceptable delay in chemotherapy initiation (UCD) as more than 42 days from LDS. Multivariate regression models were used to identify factors associated with UCD and the impact of Oncotype testing in HR+ patients. Results: Median days between LDS and chemotherapy initiation was 34 (IQR 15), with 30% of patients starting within 28 days of LDS and 23% having UCD (Table 1). Tumor characteristics such as subtype and stage affected UCD; patients with HR+ or HER2+ tumors were more likely to be delayed compared to those with TNBC. Patients with stage I disease were more likely to be delayed as well as patients undergoing mastectomy or mastectomy with reconstruction. Patients whose pathology sign-out was more than 10 days post-operatively were more likely to be delayed. A higher proportion of UCD was found in HR+ patients (31%) who received an Oncotype recurrence score compared to those who did not (20%). Table 1: Factors associated with delays in chemotherapy initiation N% DelayOdds Ratio95% CITotal52323 Age&amp;lt;4068191.00.5-2.340 to 49165161.0--50 to 59150252.01.1-3.660 to 69113282.51.3-5.070+27374.11.4-12.3RaceWhite424221.0--Non-White79271.50.8-2.7Missing20251.10.4-3.3InsurancePrivate419211.0--Public104321.60.8-2.9StageI208211.0--II243281.30.8-2.1III72110.30.1-0.7Tumor SubtypeHER2-/HR-105151.0--HR+HER2-264242.11.1-4.2HER2+154272.01.0-3.9Surgery TypeLumpectomy265161.0--Mastectomy89292.51.3-4.5Mastectomy with Immediate Reconstruction169313.31.9-5.6Pathology Sign-Out (&amp;gt;10 days)No331191.0--Yes192322.01.3-3.2Post-Op ComplicationsNo506221.0--Yes17412.20.7-6.6Clinical trial considerationNo435231.0--Yes88240.90.5-17 Conclusions: This study provides insight into populations that may be at risk to experience delays in chemotherapy initiation, directing interventions to improve the timeliness of care. Citation Format: Losk K, Vaz Duarte Luis I, Camuso K, Lloyd M, Kadish S, Hirshfield-Bartek J, Cutone L, Golshan M, Lin N, Bunnell C. Factors associated with delays in chemotherapy initiation among patients with breast cancer. [abstract]. In: Proceedings of the Thirty-Eighth Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2015 Dec 8-12; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2016;76(4 Suppl):Abstract nr P1-12-08.
Chinese-Hamster Cell Strains &lt;italic&gt;In Vitro:&lt;/italic&gt; Spontaneous Chromosome Changes and Latent Polyoma-Virus Infection&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: Denys K. Ford, Christina Boguszewski, Nelly Auersperg
Journal: JNCI Journal of the National Cancer Institute (1961)

The spontaneous changes found in the chromosome complements of 35 Chinese-hamster cell cultures are described. Unpredictable variation in chromosome count was a characteristic feature, and, after 1 to 2 years in vitro, from 10 to 30 percent of the chromosomes were aberrant. Cell cloning and improvement of the culture media failed to stabilize significantly the chromosome constitution of the cultures. Eleven sublines were inoculated with the SE polyoma virus, but it was not possible to demonstrate specific effects of the virus on the chromosome complements of the infected cultures. One inoculated subline liberated infective polyoma virus into the surrounding media continuously for 282 days, though the cultures looked healthy and proliferated normally.