tbuckley/cabot-search huggingface dataset:
AB1209 A clinical and cutaneous pathological study of 11 patients with juvenile dermatomyositis
Authors: T. Nozawa, T. Kanetaka, M. Kikuchi, T. Kizawa, T. Miyamae, T. Imagawa, K. Takahashi, S. Yokota
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Juvenile dermatomyositis (JDM) is a systemic vasculopathy with proximal muscle weakness and characteristic dermatologic eruptions. The cutaneous histopathology isn't included in DM criteria, while muscle histopathology is included in it. Recently, amyopathic dermatomyositis in childhood has been recognized to complicate with fatal interstitial pneumonia<sup>1)</sup>. Amyopathic dermatomyositis complicated with interstitial pneumonia has recently been reported even in children. The prognosis of lung manifestation is often poor. Skin biopsy is a very useful method for a diagnosis of amyopathic dermatomyositis. <h3>Objectives</h3> The purpose of this study is to evaluate association of clinical findings and features of cutaneous histology in JDM patients. <h3>Methods</h3> Fifty-eight patients (17 boys and 41 girls, age at onset 7.5±3.9 years) were diagnosed as JDM at Department of Pediatrics, Yokohama City University Hospital between 1991 and 2010. Skin biopsies from 11 patients (2 boys and 9 girls) were studied by light microscopy and Hematoxylin-eosin stained sections. The Skin biopsy sites determined by dermatologists were thighs (n=3), elbows (n=3), upper arms (n=2), trunk (n=2), the Gottron's papules of PIP joint (n=1).Clinical manifestations, laboratory findings, and histopathological features were assessed by mainly medical records retrospectively. <h3>Results</h3> Ten of eleven patients (91%) had either muscle weakness or elevated serum level of CK. The levels of fibrinogen degradation product-E (FDP-E), markers of endothelial injury, was increased in all cases and the levels of von Willebrand factor were increased in 9/11 cases (n=9, 82%). The High frequency of histological findings are vascular fibrin deposition (n=9, 82%), perivascular lymphocytic infiltration (n=9, 82%), dermal edema (n=9, 82%). The findings were also seen in one patient of amyopathic dermatomyositis with interstitial pneumonia. <h3>Conclusions</h3> Subcutaneous and dermal microvascular abnormalities; vascular fibrin depositions, vascular inflammation, of JDM were distinctive findings in this study. The cutaneous histopathology is important for amyopathic dermatomyositis. <h3>References</h3> Sato S, et al. Curr Opin Rheumatol.2010 Nov; 22(6): 639-43. <h3>Disclosure of Interest</h3> None Declared
INTRODUCTION OF ARTERIAL SHEAR STRESS SCORE AND ITS ASSOCIATION WITH CORONARY ARTERY HIGH-RISK PLAQUES AND CLINICAL EVENTS: A 3D OPTICAL COHERENCE TOMOGRAPHY STUDY
Authors: Yiannis S. Chatzizisis, Konstantinos Toutouzas, Andreas Giannopoulos, Maria Riga, Antonios Antoniadis, Yusuke Fujinom, Dimitrios Mitsouras, Charalambos Doulaverakis, Ioannis Tsamboulatidis, Ioannis Kompatsiaris, Sunao Nakamura, Frank Rybicki, Dimitris Tousoulis, Haralampos Karvounis, George Giannoglou
Journal: Journal of the American College of Cardiology (2016)
Polymorphonuclear neutrophils in periodontitis and their possible modulation as a therapeutic approach
Authors: Elena A. Nicu, Bruno G. Loos
Journal: Periodontology 2000 (2016)

Abstract The main focus of this review is polymorphonuclear neutrophilic granulocytes. Polymorphonuclear neutrophils play a pivotal role in normal host resistance to subgingival dental‐plaque biofilm. Both hyper‐ and hypo‐responsiveness of the immune system toward the microbial challenge in periodontitis have been described. We review polymorphonuclear neutrophil physiology with emphasis on the role of neutrophil functions and dysfunctions in periodontitis. Text boxes are given at the end of each subsection, which present the current knowledge on neutrophil‐modulating agents as a potential therapeutic approach in periodontitis.
A Human Colon Adenocarcinoma Xenograft—Radiation Response, Cellular Composition, and Tumor Disaggregation&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: Catharine M. L. West
 4, Peter C. Keng, Dietmar W. Siemann, Robert M. Sutherland
Journal: JNCI Journal of the National Cancer Institute (1987)

The human colon adenocarcinoma cell line WiDr was xenografted and the tumor characterized. When athymic mice (NCR-nu) were inoculated with 10(6) cells, tumors appeared after 7-14 days with a 93-100% take rate and grew with an initial volume-doubling time of around 6 days. For optimizing the tumor disaggregation method, a comparison was made of two dissociation procedures and of different dissociation times. An enzyme cocktail (collagenase, DNase, pronase) resulted in total viable cell yields of 1-3 X 10(7) cells/g tumor tissue. Cell yield decreased with increasing tumor weight. Disaggregation with trypsin gave lower cell yields; and so, although the plating efficiencies (PEs) were higher, the enzyme cocktail was chosen for tumor disaggregation. On the basis of morphologic identification, cell suspensions prepared from WiDr tumors, by use of the enzyme cocktail for 2 hours, contained 49% malignant cells as well as a significant fraction of nonneoplastic cells. The major nonneoplastic host cell component was macrophage (33%); lymphocytes (13%) and granulocytes (5%) also were present. Host cells could be separated from neoplastic cells by centrifugal elutriation. By mixing various proportions of host and tumor cells, it was subsequently shown that the presence of host cells did not influence the malignant cell PE unless the cell suspensions contained greater than 90% host cells. Single-cell suspensions prepared from WiDr tumors, with use of the enzyme cocktail for 2 hours, were irradiated and then plated for survival (D0 = 1.5 Gy; n = 5) (D0, the 37% dose slope). A comparison was made of the sensitivity to radiation, after the different dissociation methods. The radiation sensitivities after 1.5-hour trypsinization and 2- and 6-hour enzyme cocktail administrations were similar, but after 0.5 hour of trypsin, the cells were more sensitive to radiation.
Immunoblastic Lymphadenopathy
Authors: Robert J. Lukes, Barbara H. Tindle
Journal: New England Journal of Medicine (1975)

Immunoblastic lymphadenopathy, although it resembles Hodgkin's disease, is a distinct, hyperimmune disorder apparently of the B-cell system. In 32 cases, it was characterized by a morphologic triad: proliferation of arborizing small vessels; prominent immunoblastic proliferations; and amorphous acidophilic interstitial material. Clinically, it is manifested by fever, sweats, weight loss, occasionally a rash, generalized lymphadenopathy and often hepatosplenomegaly. There is a consistent polyclonal hyperglobulinemia and often hemolytic anemia. The course of the disease is usually progressive, with a median survival of 15 months in 18 fatal cases. The cellular proliferation appears benign morphologically in the pretherapy biopsies and in 10 of 12 available autopsy cases. In three cases the process evolved into a lymphoma of immunoblasts, immunoblastic sarcoma. The basic process appears to be a non-neoplastic hyperimmune proliferation of the B-cell system involving an exaggeration of lymphocyte transformation to immunoblasts and plasma cells that may be triggered by a hypersensitivity reaction to therapeutic agents.
P16.03 * UNIFYING CLINICAL ROUTINE BRAIN TUMOR MR-SPECTROSCOPY AND MR-IMAGE ANALYSIS: NOVEL JMRUI PLUG-INS FOR BRAIN TUMOR ANALYSIS
Authors: N. Barros, M. Jablonski, A. Pica, J. Starcukova, U. Knecht, R. Wiest, J. Slotboom
Journal: Neuro-Oncology (2014)

INTRODUCTION: Magnetic resonance imaging and spectroscopy are the neuroradiological methods of first choice for the diagnostics of de novo brain tumors, the evaluation tumor response to therapy and the tumor progression. Whereas (i.) T2 and post contrast T1-weighted MRI gives information on the brain tumors' anatomy and integrity of the blood brain barrier, (ii.) perfusion weighted MRI on the perfusion state (important to study processes like neo-angiogenis, apoptosis and necrosis), (iii.) diffusion weighted MRI (evaluation for cellular density, and white matter tract integrity), information on the tumors' metabolism is obtained by MR-spectroscopy. Lactate gives information on the ischemic state, choline/ mobile lipids on the membrane turnover and necrosis/apoptosis. The lactate, as present in many high grade glioma, is an indicator an ischemic condition which can ge regarded as an indicator for tumors resistance to radiation therapy. However, despite the fact that MRS gives valuable additional information to MRI, the available software for clinical routine analysis of MRS data together with MRI data is far from ideal. This abstracts reports on the software, which is currently being developed within an EU-funded Marie Curie Initial Training Network (ITN) (http://www.transact-itn.eu/) named TRANSACT which stands for “Transforming Magnetic Resonance Spectroscopy into a Clinical Tool”. METHODS: Basis for the developed software within the TRANSACT-project is the software package jMRUI, which was developed during former EU-funded projects, and targeted mainly on scientific users of MR-spectroscopy; the current TRANSACT project however focuses on the develop software for clinicians. The novel plug-ins for jMRUI were entirely developed in JAVA. RESULTS: The following important clinical work flow related requirements were incorporated: (a.) full support of DICOM image and spectroscopy data format; (b.) a fully featured integrated patient/study/series examination browser; (c.) automatic projection of spectral voxels within automatically loaded reference image stacks; (d.) DICOM transfer using the standard DICOM network data transfer protocol; (e.) DICOM reporting of spectroscopy results, and possibility to transfer of these results into PACS systems; (f.) absolute quantification of single voxel spectra; (g.) advanced display of spectroscopic SVS and MRSI data; (h.) automated fast metabolite image generation (i.) easy correlation of numeric image data and parametric spectral data. CONCLUSION: An intuitive software has been developed for a clinical setting that allows the simutaneous study of MRI/MRS brain tumor data.
Abstract PD09-04: Weight Loss in Postmenopausal Women Is Associated with Modulation of Serum and Tissue Based Risk Biomarkers
Authors: CJ Fabian, BF Kimler, TA Phillips, CM Zalles, JR Klemp, LM Malone, SD. Hursting
Journal: Cancer Research (2010)

Abstract Background In a pilot study, a structured program of reduced energy diet, physical activity, and weekly group behavioral intervention with other high risk women was successful at producing a median 11% weight loss with at least a 5% weight loss in 88% of subjects. We evaluated the association of weight loss with changes in serum and breast tissue risk and mechanisms of action biomarkers. Methods High risk postmenopausal women with BMI &amp;gt;25 kg/m2 had breast tissue harvested by random periareolar fine needle aspiration (RPFNA) before and after a 6-month energy balance intervention. Specimens were evaluated for biomarkers including cytomorphology, proliferation (immunocytochemical Ki-67), gene expression by RT-qPCR, and expression of cytokines and adpokines by Luminex assay. Fasting serum was assayed for insulin, glucose, adiponectin, leptin, high sensitivity CRP, IL-6, prolactin, SHBG, estradiol and testosterone using ELISA or Luminex. Results For 24 biomarker evaluable subjects, 21 had &amp;gt;5% weight loss (median = 11%). Cytologic atypia was present in 10/24 at baseline and 4/24 at 6 months (p=0.034). For 20 subjects with sufficient cells for assessment of Ki-67 at both times, median baseline Ki-67 was 0.7% and off study 0.3%, with a median change of -0.2% (p=0.19). Statistically significant changes (≥0.003; Wilcoxon) were observed for serum levels of adiponectin, adiponectin:leptin ratio, and SHBG (increases); and leptin, bioavailable estradiol and hsCRP (decreases). Reduction was also observed for insulin (p=0.018) and bioavailable testosterone (p=0.033). These results were duplicated (p≥0.014) by Luminex for adiponectin, leptin, adiponectin: leptin ratio, and insulin; plus hepatocyte growth factor (HGF, decrease). Also, an increase in the adiponectin:leptin ratio was observed for the RPFNA specimens (p=0.012). Gene expression (RT-qPCR) of pS2 was significantly modified (decrease, p=0.035). Further, the weight loss (expressed as relative change) was highly statistically correlated with change (relative) in serum leptin, adiponectin:leptin ratio, SHGB, and free estradiol; as well as with relative change in adiponectin:leptin ratio in RPFNA specimens. Conclusion Weight loss in high risk postmenopausal women is accompanied by significant modulation of numerous serum and breast tissue-based biomarkers. For several risk and response biomarkers there is a significant correlation between change in the biomarker and the weight loss achieved. This suggests the possibility of identifying mechanisms of action and signaling pathways for dietary/energy balance interventions that may reduce risk for development of breast cancer. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr PD09-04.
Scoliosis deformity correction: Patients pre-operative anxiety and the desire for more information
Authors: S. Ismael, M. Kazeem, A. Gibson
Journal: International Journal of Surgery (2015)

s / International Journal of Surgery 23 (2015) S15eS134 S84 0401: NECK OF FEMUR INFECTIONS: RETROSPECTIVE STUDY 2013/2014 F. Ibram, J. Fountain. Aintree University Hospital, UK Aim: Wound infections in post-operative neck of femur (NOF) patients increase morbidity and mortality. Given a recent surge in NOF infections at Aintree University Hospital (AUH), the aims of this studywere to assess our NOF infection rates compared to national standards and identify trends in current practice. Methods: Retrospective study of NOF patients with confirmed surgical site infections (SSI) during July 2013eSeptember 2014. Data collected from notes, Sigma & JAC. National SSI data obtained from Public Health England. Results: During July 2013eJune 2014, 8NOF patients developed SSIs (2.5%) compared to national standard (1.6%). An additional 3 cases (2.8%) were reported during JulyeSeptember 2014, however this data could not be compareddue to unavailable national standards. Of these 11 SSIs, 82%were deep and 18%were superficial. Deep infections were all eventually treated with girdlestones, either as primary (44%) or secondary procedure (56%). Girdlestones performed as a primarymeasure reduced hospital admission by 35 days and antibiotic duration by 20 days suggesting deep NOF infections should be treated with this intervention as first line therapy. Conclusion: AUH NOF infections were 1.6-fold higher compared to national standards. Girdlestone procedures in deep infections are most effective if performed initially. 0496: SCOLIOSIS DEFORMITY CORRECTION: PATIENTS PRE-OPERATIVE ANXIETY AND THE DESIRE FOR MORE INFORMATION S. Ismael, M. Kazeem, A. Gibson. The Royal National Orthopaedic Hospital,
Abstract 4706: Wide surgical margins are of prognostic importance in GIST.
Authors: Jan Ahlen, Robert Branstrom, Inga-Lena Nilsson
Journal: Cancer Research (2013)

Abstract Background: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumor of the gastrointestinal tract and even in the era with molecularly targeted agents is surgery the mainstay of treatment and the only treatment that could lead to cure. Microscopically negative margins are today accepted as sufficient margins for cure while tumor rupture is known to result in poor prognosis and therefore taken into account in prognostic models. In other types of soft tissue sarcoma has wide margins been known to be of importance for recurrence while this has not been shown in GIST. Methods: 116 patient were included in the study, 55 male and 61 female, mean age 61 years (10-86) and mean follow-up 73 month (4,6-365) that has been evaluated for surgical margins at the time for surgery or at referral to our institution. The criteria used has been those used for sarcoma in other locations were “Wide margins” are defined as 3 cm marginal in the organ of origin combined with an completely intact peritoneal surface covering the tumor. In cases where the tumor is stucked to surrounding tissue or organ, that part has been resected “un bloc” with the tumor. “Marginal margins” are defined as R0 resection with less then 3 cm and “Intralesional margin” is when the tumor is shelled out or damage to surrounding peritoneal surface or judged as R1 resection. Local recurrence has been defined as recurrence at site of origin or intraabdominal recurrence of tumor in the peritoneum and metastatic disease when recurrence is distant metastasis. Results: We found 48 cases with wide margins(3 recurrence), 39 with marginal margins (15 recurrence) and 29 with intralesional margins (17 recurrence). There was significant difference calculated as Kaplan-Meier for both local recurrence (p=0,00016), distant metastasis (p= 0,004) and survival (p= 0,0047) and in cox regression analysis hazard ratio were 7,4 for marginal margins and 8,08 for intralesional margins compared to wide margins and this was not correlated with size or site (p= 0,007). Conclusion: Surgical margins is of great importance in GIST both for local recurrence, distant metastasis and survival and patient with GIST should be operated with wide margins, in accordance with principals of other sarcoma. Citation Format: Jan Ahlen, Robert Branstrom, Inga-Lena Nilsson. Wide surgical margins are of prognostic importance in GIST. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4706. doi:10.1158/1538-7445.AM2013-4706
Impaired Processing of Prohormones Associated with Abnormalities of Glucose Homeostasis and Adrenal Function
Authors: Stephen O'Rahilly, Helen Gray, Phillippa J. Humphreys, Anna Krook, Kenneth S. Polonsky, Anne White, Sarah Gibson, Kevin Taylor, Colin Carr
Journal: New England Journal of Medicine (1995)

Insulin, the central hormone controlling the utilization of fuel, is initially synthesized in pancreatic beta cells as its relatively inactive precursor, proinsulin. A series of enzymes that process prohormones act on proinsulin to produce mature insulin and C peptide. Abnormalities in the secretory products of beta cells are a rare but well-documented cause of impairment in glucose tolerance and hyperinsulinemia.1 Two specific syndromes have been defined. First, mutations in the insulin gene that lead to the production of biologically ineffective insulin have been described in several families.2–4 All cases described to date have involved heterozygotes who produce normal insulin . . .
SAT0220 Measurement of Serum Leucine-Rich Alpha-2 Glycoprotein, A Novel Disease Activity Biomarker in Rheumatoid Arthritis, for the Detection of Biologic-Associated Tuberculosis
Authors: T. Ohkawara, M. Fujimoto, S. Serada, T. Naka
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Tuberculosis is considered to be a severe complication during biologic therapies in patients with rheumatoid arthritis (RA). Importantly, conventional inflammatory biomarkers such as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) are often normalized by biologic therapies, particularly by IL-6 blockade, but an alternative biomarker for tuberculosis detection remains to be established. Previously, by proteomic approach using sera from RA patients, we identified leucine-rich alpha-2 glycoprotein (LRG) as a novel disease activity marker for RA. Interestingly, serum LRG levels are also elevated in other immune disorders such as Behcet9s disease and inflammatory bowel diseases, suggesting that LRG is a biomarker for inflammation. <h3>Objectives</h3> We investigated whether LRG is potentially useful for detecting tuberculosis during biologic therapies. <h3>Methods</h3> Serum samples were harvested from tuberculosis patients (n=33) without biologic therapies before and after anti-tuberculosis treatment. Serum LRG levels were determined by ELISA. To recapitulate tuberculosis development during biologic therapies in RA patients, a murine model of mycobacterium (BCG) infection with or without anti-IL-6 receptor antibody treatment was used and sera were harvested for ELISA analysis of murine LRG. <h3>Results</h3> In tuberculosis patients before anti-tuberculosis therapy, serum LRG levels were significantly elevated compared to those of healthy controls. In addition, serum LRG were significantly reduced 1 month after therapy and were further reduced 3 months after therapy. As in humans, serum LRG levels were elevated in mice with various inflammatory conditions including collagen-induced arthritis and mycobacterium infection. When mice were treated with anti-IL-6R antibody and were subjected to mycobacterial infection, serum LRG levels in these mice were similarly elevated as those in mice treated with control antibody. <h3>Conclusions</h3> Our analysis of tuberculosis patients indicates that LRG can be a novel biomarker for tuberculosis. Our analysis of an experimental model suggests that LRG is useful for detecting mycobacterial infection even in the suppression of IL-6 by biologic therapies, although this must be confirmed in human studies. The measurement of serum LRG may thus be useful for the simultaneous monitoring of arthritis activity and tuberculosis infection. <h3>References</h3> TRAQ-based proteomic identification of leucine-rich alpha-2 glycoprotein as a novel inflammatory biomarker in autoimmune diseases. Ann Rheum Dis. 2010 Apr;69(4):770-4 <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4017
METABOLIC COST OF UNLOADED VERSUS RAMP EXERCISE: MECHANISMS OF EXERCISE INTOLERANCE IN HEART FAILURE PRESERVED EJECTION FRACTION
Authors: Stephanie Meller, Neal Chatterjee, Bishnu Dhakal, Paul Pappagianopoulos, Aaron Eisman, Cole S. Bailey, Aaron Baggish, Rajeev Malhotra, Gregory Lewis
Journal: Journal of the American College of Cardiology (2016)
UTILIDAD DE LA ECOENDOSCOPIA EN EL DIAGNÓSTICO DE LAS LESIONES SÓLIDAS PANCREÁTICAS. EXPERIENCIA EN NUESTRO CENTRO
Authors: B Merino Rodríguez, C González Asanza, O Nogales Rincón, A Huerta Madrigal, A Cruz Moratinos, E Cos Arregui, P Menchén Fernández Pacheco
Journal: Endoscopy (2010)

Introducción: La ecoendoscopia es el método de referencia para el estadiaje locoregional de los tumores pancreáticos. Cada vez es más necesario una confirmación anatomopatológica de estas lesiones con vistas a establecer un esquema de tratamiento oncológico (paliativo o adyuvante)