tbuckley/cabot-search huggingface dataset:
Clonal Hematopoiesis Demonstrated by X-Linked DNA Polymorphisms after Allogeneic Bone Marrow Transplantation
Authors: Ali G. Turhan, R. Keith Humphries, Gordon L. Phillips, Allen C. Eaves, Connie J. Eaves
Journal: New England Journal of Medicine (1989)

We analyzed DNA from peripheral-blood and marrow cells from 12 recipients of allogeneic bone marrow transplants to determine whether monoclonal but otherwise normal hematopoiesis occurs in such patients. All patients were being treated for various forms of leukemia or lymphoma. In 10 patients, granulocytes isolated from peripheral-blood samples obtained 28 to 159 days after transplantation were polyclonal. In some, circulating T cells were isolated and also found to be polyclonal. In contrast, two patients had donor-derived monoclonal or oligoclonal hematopoiesis after transplantation. In one, DNA from circulating mononuclear cells obtained 29 days after transplantation revealed a monoclonal pattern on analysis of a restriction-fragment-length polymorphism in the phosphoglycerate kinase gene. In the other, analysis of a restriction-fragment-length polymorphism in the hypoxanthine phosphoribosyltransferase gene suggested the presence of a dominant clone in the granulocytes sampled 36 days after transplantation. When the latter patient was reassessed on day 267, the same clone of donor hematopoietic cells was still predominant and was found to include circulating T cells as well as granulocytes. We conclude that monoclonal hematopoiesis of donor origin may be observed in recipients of allogeneic bone marrow transplants, indicating that stem cells in normal adult human marrow are able to repopulate both lymphoid and myeloid compartments after transplantation.
Natural History of High-Risk Bundle-Branch Block
Authors: John H. McAnulty, Shahbudin H. Rahimtoola, Edward Murphy, Henry DeMots, Leonard Ritzmann, Paula E. Kanarek, Susan Kauffman
Journal: New England Journal of Medicine (1982)

We conducted a prospective study in which 554 patients with chronic bifascicular and trifascicular conduction abnormalities were followed for an average of 42.4 +/- 8.5 months. Heart block occurred in 19 patients, and 17 were successfully treated. The actuarial five-year mortality from an event that could conceivably have been a bradyarrhythmia was 6 per cent (35 per cent from all causes). Of the 160 deaths 67 (42 per cent) were sudden; most of these were not ascribable to bradyarrhythmia but to tachyarrhythmia and myocardial infarction. Mortality was higher in patients with coronary-artery disease (P less than 0.01) and congestive heart failure (P less than 0.05). Patients in whom syncope developed before or after entry into the study had a 17 per cent incidence of heart block (2 per cent in those without syncope)(P less than 0.05); however, no single variable was predictive of which patients were at high risk of death from a bradyarrhythmia. The predictors of death were increasing age, congestive heart failure, and coronary-artery disease; the predictors of sudden death were coronary-artery disease and increasing age. The risks of heart block and of death from a bradyarrhythmia are low; in most patients, heart block can be recognized and successfully treated with a pacemaker.
Is ascitic fluid interleukin-12 an independent prognostic factor in ovarian cancer? The necessity of correction milligram P values for multiple comparisons.
Authors: A P Kudelka, R Lenzi, E N Atkinson, L B Levy, R S Freedman
Journal: Journal of Clinical Oncology (1998)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1998.16.9.3208 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 9738599 Is ascitic fluid interleukin-12 an independent prognostic factor in ovarian cancer? The necessity of correction milligram P values for multiple comparisons. A P KudelkaxA P KudelkaSearch for articles by this author , R LenzixR LenziSearch for articles by this author , E N AtkinsonxE N AtkinsonSearch for articles by this author , L B LevyxL B LevySearch for articles by this author , R S FreedmanxR S FreedmanSearch for articles by this author https://doi.org/10.1200/JCO.1998.16.9.3208 PDF "Is ascitic fluid interleukin-12 an independent prognostic factor in ovarian cancer? The necessity of correction milligram P values for multiple comparisons.." Journal of Clinical Oncology, 16(9), pp. 3208–3209© 1998 by American Society of Clinical Oncology
Abstract 3321: Cationic polyurethanes-short branch PEI-mediated delivery of Mir145 inhibited epithelial-mesenchymal transdifferentiation and cancer stem-like properties and in lung adenocarcinoma
Authors: Chian-Shiu Chien, Shih-Hwa Chiou
Journal: Cancer Research (2012)

Abstract The high invasiveness and frequent recurrence of lung adenocarcinoma (LAC) are major reasons for treatment failures and poor prognoses. Alterations in microRNAs (miRNAs) expression have been shown in lung cancers. Recent reports have demonstrated that tumors contain a small subpopulation of cancer stem cells (CSCs) that possesses self-renewing capacity and is responsible for tumor malignancy including metastasis, relapse, and chemoradioresistance. However, a miRNAs-based therapeutic approach in LAC-associated CSCs (LAC-CSCs) is still blurred. Using miRNA/mRNA-microarray and Quantitative RT-PCR, we found that the expression of miR145 is negatively correlated with the levels of Oct4/Sox2/Fascin1 in LAC patient specimens, and an Oct4highSox2highFascin1highmiR145low phenotype predicted poor prognosis. We enriched LAC-CSCs by side population sorting or identification of CD133 markers and found that LAC-CSCs exhibited low miR145 and high Oct4/Sox2/Fascin1 expression, CSC-like properties, and chemoradioresistance. To clarify the role of miR145, we used a polyurethane-short branch-polyethylenimine (PU-PEI) as the vehicle to deliver miR145 into LAC-CSCs. PU-PEI-mediated miR145 delivery reduced CSC-like properties, and improved chemoradioresistance in LAC-CSCs by directly targeting Oct4/Sox2/Fascin1. Importantly, the repressive effect of miR145 on tumor metastasis was mediated by inhibiting the epithelial-mesenchymal transdifferentiation (EMT) and metastastic ability, partially by regulating Oct4/Sox2/Fascin1, Tcf4, and Wnt5a. Finally, in vivo study showed that PU-PEI-mediated miR145 delivery to xenograft tumors reduced tumor growth and metastasis, sensitized tumors to chemoradiotherapies, and prolonged the survival times of tumor-bearing mice. Our results demonstrated that miR145 acts as a switch regulating lung CSC-like and EMT properties, and provide insights into the clinical prospect of miR145-based therapies for malignant lung cancers. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3321. doi:1538-7445.AM2012-3321
Prevention of Illness from Rhinovirus Infection by a Topical Interferon Inducer
Authors: Charoen Panusarn, Edith D. Stanley, Victor Dirda, Mary Rubenis, George Gee Jackson
Journal: New England Journal of Medicine (1974)

A double-blind, placebo-controlled study was performed to observe the effect of a topical interferon inducer in the prevention of rhinovirus infection and illness in 39 young adults. The drug, a substituted propanediamine, was administered as nose drops for two days, beginning one day before challenge with rhinovirus Type 21. Interferon subsequently appeared in nasal washings in a high titer. Among antibody-free persons drug treatment, as compared to placebo, decreased infectivity of the virus, markedly reduced symptomatic illness, and decreased virus shedding if infection occurred. Among subjects with prechallenge antibody, drug treatment further increased resistance to infection, decreased virus recovery, and prevented symptoms. The data show a statistically significant drug-related and interferon-related protection against illness caused by rhinovirus Type 21, a common-cold virus. (N Engl J Med 291:57–61, 1974)
APPLICATION AND OUTCOMES OF THE HYBRID APPROACH TO CHRONIC TOTAL OCCLUSION PERCUTANEOUS CORONARY INTERVENTION: AN UPDATE FROM A CONTEMPORARY MULTICENTER U.S. REGISTRY
Authors: Aris Karatasakis, Barbara Danek, Dimitrios Karmpaliotis, Khaldoon Alaswad, Farouc Jaffer, Robert Yeh, Mitul Patel, Ehtisham Mahmud, David Kandzari, James Choi, Nicholas Lembo, Anthony Doing, Catalin Toma, Ray Wyman, William Lombardi, Barry Uretsky, Santiago Garcia, Jeffrey Moses, Ajay Kirtane, Manish Parikh, Judit Karacsonyi, Bavana Rangan, Subhash Banerjee, Emmanouil Brilakis
Journal: Journal of the American College of Cardiology (2017)
SP0007 The Total Knee Prosthesis as Ultimate (At Least Final) Outcome Measure in OA Trials
Authors: L. Gossec
Journal: Annals of the Rheumatic Diseases (2014)

<h3></h3> Osteoarthritis (OA) is a major cause of disability worldwide. Over the past years, interest has grown among the scientific community, pharmaceutical companies, and regulatory agencies in the development of drugs that might influence the natural history of structural changes in OA by preventing, retarding, or reversing cartilage breakdown. To be able to assess such drugs, it is essential to identify a valid outcome variable that reflects the natural history of structural changes in OA. Requirement of total joint replacement (TJR) is an interesting outcome, as it is a "hard" endpoint. Limitations exist, however, in the use of this outcome. In this presentation, we will discuss the advantages and limitations of the requirement of TJR as an outcome measure, as well as its alternative: "fulfilling the criteria for TJR". <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.6174
Cold Spots on Bone Scans in Patients Wearing Weighted Breast Prostheses
Authors: DIETLIND L. WAHNER-ROEDLER, GREGORY A. WISEMAN, WILLIAM L. DUNN
Journal: Clinical Nuclear Medicine (1996)

A bone scan is frequently obtained in patients with breast cancer for evaluation of possible metastatic bone disease or for evaluation of response of known metastases to treatment. These patients commonly wear breast prostheses after unilateral or bilateral mastectomies. On some bone scans, the authors have observed artifacts resulting from certain breast prostheses. In particular, in the patient described, a weighted breast prosthesis was not removed during Tc-99m HMDP bone scanning and was interpreted as being permanent pacemaker or defibrillation unit. The scintigraphic appearance of several commonly used, commercially available weighted breast prostheses is discussed.
<i>Helicobacter pylori</i>Infection and the Risk of Gastric Carcinoma
Authors: Julie Parsonnet, Gary D. Friedman, Daniel P. Vandersteen, Yuan Chang, Joseph H. Vogelman, Norman Orentreich, Richard K. Sibley
Journal: New England Journal of Medicine (1991)

Infection with Helicobacter pylori has been linked with chronic atrophic gastritis, an inflammatory precursor of gastric adenocarcinoma. In a nested case–control study, we explored whether H. pylori infection increases the risk of gastric carcinoma.
Abstract 5218: Basigin-2 (EMMPRIN), a prognostic marker, is a dynamic portal of entry into cancer cells
Authors: Leonardo R. Ancheta, Douglas A. Lappi
Journal: Cancer Research (2011)

Abstract Basigin-2 (EMMPRIN) is a marker for prognosis of several tumor types. With the identification of the internalization of basigin-2 in response to antibody binding, we now further explore the possibility of its use as a dynamic portal of entry for molecules into cancer cells. We also wish to identify a correlation between number of basigin-2 on the cell surface and half maximal effective concentration (EC50) of a targeted toxin by examining eleven tumor cell lines which encompass various tumor types such as breast (MCF-7, BT549), leukemia (K562, HL-60), multiple myeloma (RPMI-8226), neuroblastoma (SH-SY5Y), non-small cell lung (A549, NCI-H23, NCI-H522), and prostate (PC3). In this investigation, we employed the use of flow cytometry to characterize each cell line according to mean fluorescence as well as to determine the number of basigin-2 molecules expressed on the cell surface through a surface quantification assay. This was visualized by staining with a monoclonal antibody directed to basigin-2 ECD with comparison of results against a standard with known Antibody Binding Capacity (ABC) and standardizing the results into Molecules of Equivalent Soluble Fluorochromes (MESF; Bangs Laboratories, Inc.). Last year at this meeting we showed the ability of an antibody to basigin-2 ECD to cause internalization of secondary targeted toxins to cause cell death. We now report on the activity of a primary toxin: the ribosome-inactivating protein saporin attached directly to an antibody to basigin-2. Cytotoxicity assays yielded an EC50 range of 3.5-420 pM while surface quantification assays resulted in expression that ranged from 1-4 million basigin-2 molecules per cell. Cell lines showing potent EC50 values were characterized to have higher surface expression of basign-2 when compared to cell lines with less potent EC50 values. Preliminary results suggest a correlation between EC50 and basigin-2 surface expression such that more basigin-2 available on the cell surface would facilitate greater opportunity for a primary toxin to bind and enter into the cell and cause cell death. These data suggest the use of basigin-2 as a means to identify an entry system for molecules into cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5218. doi:10.1158/1538-7445.AM2011-5218
Controlled Studies of Streptococcal Pharyngitis in a Pediatric Population
Authors: Alan C. Siegel, Eloise E. Johnson, Gene H. Stollerman
Journal: New England Journal of Medicine (1961)

PROMPT and adequate penicillin therapy of A pharyngitis due to Group A streptococci has proved effective in preventing primary attacks of rheumatic fever in military populations.1 , 2 Such treatment has been widely recommended for the control of rheumatic fever in the civilian population as well.3 The attack rate of rheumatic fever after such infections has been determined primarily from studies of epidemic, exudative pharyngitis or scarlet fever.4 5 6 Approximately 3 per cent of these infections have been followed by rheumatic fever.7 Within a given population, however, this rate has varied directly with the degree of the host's immune response to streptococcal disease. . . .
P58 A Comparison Between The Clinical Features Of Pisz And Pizz Patients With Alpha-1 Antitrypsin Deficiency
Authors: S. Vayalapra, R. Edgar, D. Griffiths, R. Stockley, A. Turner
Journal: Thorax (2014)

<h3>Introduction</h3> Alpha-1 antitrypsin deficiency (AATD) is a hereditary disorder affecting about 1 in 3000 people in the UK commonly associated with early-onset emphysema. There are two common deficiency alleles - PiS and PiZ. PiZZ patients have severe AATD, with levels of 10–15% normal. PiSZ patients have less severe deficiency (≈ 40% normal) and are generally thought to have a minimal risk. We hypothesised that if PiSZ patients were at lower risk of COPD than PiZZ, and their lung disease would be more characteristic of usual COPD than that of PiZZ patients. <h3>Method</h3> 104 PiSZ patients and 638 PiZZ patients from the UK AATD registry (ADAPT) were compared for their demographics, lung function, risk factors for COPD (e.g. smoking, occupation), co-morbidities associated with COPD, index status (i.e. if diagnosed due to lung disease or family screening) and CT densitometry (where available). Outcome in terms of lung function decline and mortality was also assessed. Univariate statistics were used to guide subsequent regression analyses. <h3>Results</h3> Emphysema was more likely in PiZZ than PiSZ patients (OR 11.0 (5.7–21.3); p &lt; 0.001) in the regression analysis after accounting for age, pack years and lung index status. PiZZ patients also had significantly worse FEV1 and DLCO than PiSZ patients in similar regression models (both p &lt; 0.01). Emphysema was more severe in both upper and lower zone (both p &lt; 0.01), and proportionately greater in the lower zone (UZ/LZ VI = 1.5 v 1.2) in PiZZ patients. Mortality and DLCO decline were also greater in PiZZ patients. <h3>Conclusion</h3> PiSZ patients have a milder form of AATD associated with better lung function. The data suggests the pattern of emphysema is closer to usual COPD than classical AATD. Further analyses comparing PiSZ to PiMM are now ongoing.
AB0336 Clinical significance of serial measurement of anti-citrullinated protein antibodies (ACPA) in patients with palindromic rheumatism
Authors: S.R. Cabrera-Villalba, V. Ruiz-Esquide, J.A. Gomez-Puerta, M.V. Hernandez, G. Salvador, J.D. Cañete, R. Sanmarti
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Anti-citrullinated protein antibodies (ACPA), the most specific markers of rheumatoid arthritis (RA), are found in approximately 50% of patients with palindromic rheumatism (PR). A significant proportion of these patients may evolve to RA. <h3>Objectives</h3> To analyze the clinical significance of serial measurements of ACPA in patients with PR as biomarkers of progression to RA. <h3>Methods</h3> All patients diagnosed with PR (Guerne et al criteria <sup>1</sup>), attended by our Arthritis Unit were included. We analyzed only patients considered as pure PR (without evidence of persistent arthritis or other chronic rheumatic disease at the first measurement of ACPA). ACPA were analyzed in serum by commercial tests; CCP1 (until 2002) and CCP2 (from 2003). Clinical data were collected during follow-up, establishing the date of diagnosis of RA (1987 ACR criteria) or other rheumatic diseases. <h3>Results</h3> Seventy-one patients (54F/17M) with an initial diagnosis of pure PR and a mean follow up time of 7.6±4.7 years (range 1.1-17.7) were included after the first determination of ACPA. In 57 (43F/14M) patients, ≥2 serial measurements of ACPA were available. At the first ACPA measurement, 35 patients were ACPA positive and 22 ACPA negative. Fourteen patients (24.6%) evolved to RA after a mean of 8.5±5.1 years. ACPA positive patients more-frequently developed RA than ACPA negative patients (31.4% vs 13.6%, p 0.2). Thirty-eight patients (66.7%) remained with a diagnosis of PR and 8.8% evolved to another rheumatic disease. Forty patients (70.2%) were treated with antimalarials (chloroquine or hydroxychloroquine). The mean ACPA serum measurement was 3.3±1.5 (range 2-8) and in 64.9% of patients 2-3 determinations were made. The mean time from the first to the last measurement was 70.3±49.9 months. ACPA remained positive in successive measurements in all patients ACPA positive at baseline. ACPA levels remained stable during follow-up (mean of 744.9±598.4 IU at the first and 629.1±528.5 IU in the last measurement, p&gt;0.05. Seroconversion occurred at 11 and 18 months of follow up, respectively, in two of the 22 patients ACPA negative at baseline, one of whom evolved to RA. <h3>Conclusions</h3> ACPA are common in the sera of patients with PR and although ACPA positivity is a risk factor of progression to RA, a significant number of patients with positive ACPA do not evolve to RA or another chronic rheumatic disease during a long term follow-up. ACPA serum levels remained stable over time and seroconversion is uncommon after the onset of symptoms of RP. <h3>References</h3> Guerne PA, Weissman MH, AM J Med.1992:93:451-60. <h3>Disclosure of Interest</h3> None Declared
Autoantibodies Cytotoxic to Gastric Parietal Cells in Serum of Patients with Pernicious Anemia
Authors: Henry J. De Aizpurua, Leah J. Cosgrove, Berta Ungar, Ban-Hock Toh
Journal: New England Journal of Medicine (1983)

We measured the complement-dependent cytotoxic activity of serum in 60 patients with pernicious anemia. Canine gastric mucosal cells served as the indicator of cytotoxicity, which was expressed as a percentage of the maximal effect produced by a cytolytic agent (Triton X-100). Serum from patients with pernicious anemia showed a higher average activity (11.8 +/- 10.3 per cent, P less than 0.001) than serum from 29 patients with systemic lupus erythematosus (1.0 +/- 1.8 per cent), 10 with scleroderma (0.1 +/- 0.1 per cent), 10 with rheumatoid arthritis (0.6 +/- 0.6 per cent), 22 with multiple sclerosis (0.4 +/- 1.2 per cent), and 23 with chronic persistent hepatitis (0.03 +/- 0.1 per cent), and serum from 64 healthy persons (0.4 +/- 1.0 per cent). Serum from patients with pernicious anemia was not toxic to canine liver or kidney cells. Absorption with gastric mucosal cells and heat inactivation of complement abolished the cytotoxic reaction. The cytotoxic factor resided in the IgG fraction of immunoglobulin, and the amount of cytotoxicity was proportional to the IgG concentration. Cytotoxic activity correlated with the presence of parietal-cell-surface--reactive autoantibody demonstrated by immunofluorescence. We conclude that cytotoxic autoantibodies to parietal cells may contribute to the loss of such cells from the gastric mucosa of patients with pernicious anemia.
Chromosome Number and Morphology of Benign Ovarian Cystic Teratomas
Authors: Philip A. Corfman, Ralph M. Richart
Journal: New England Journal of Medicine (1964)

TERATOMAS have been of special interest for many years, and their genesis, variously attributed to incited totipotential cells, misplaced blastomeres and parthenogenesis, has been the subject of controversy. Willis1 summarized the data that indicated to him that these tumors are not malformed fetuses derived either from misplaced blastomeres or by parthenogenesis but, in fact, true neoplasms derived from cells that, early in development, escape from the action of primary organizers. Hunter and Lennox,2 to confirm Willis's theory that teratomas originate solely from host somatic cells, used the sex-chromatin technic first described by Barr and Bertram3 and examined 8 testicular teratomas. . . .
Proposed targets for new NHS commissioners receive lukewarm response
Authors: A. O'Dowd
Journal: BMJ (2012)

Proposed targets for the doctor led clinical commissioning groups that will take charge of commissioning in the NHS in England from next year have received a lacklustre initial response.

The National Institute for Health and Clinical Excellence (NICE) working with the NHS Commissioning Board published the first menu of Commissioning Outcomes Framework (COF) indicators on 1 August.1

The 44 indicators recommended by the COF advisory committee include targets on cancer mortality, care after a stroke, emergency admissions, and patients’ quality of life.

From April next year, the COF will hold clinical commissioning groups to account for improvement in quality of healthcare and patient outcomes.

Several doctors’ representatives and charities have raised doubts over the indicators saying there are too …
REGIONAL CEREBRAL GLUCOSE USE IN PATIENTS WITH ALCOHOLIC KORSAKOFFʼS SYNDROME.
Authors: R. M. Kessler, C. M. Clark, E. S. Parker, P. Martin, L. Sokoloff, M. H. Ebert, M. Mishkin
Journal: Clinical Nuclear Medicine (1984)

Kessler, R. M.; Clark, C. M.; Parker, E. S.; Martin, P.; Sokoloff, L.; Ebert, M. H.; Mishkin, M. Author Information