tbuckley/cabot-search huggingface dataset:
Analyse du risque de complication hémorragique au décours de la mucosectomie duodénale
Authors: S Tahri, G Bouguen, M Pagenault, PN d'Halluin, S Manfredi, L Siproudhis, JF Bretagne
Journal: Endoscopy (2011)

Introduction: La mucosectomie endoscopique est une méthode reconnue pour la résection des lésions néoplasiques superficielles de l'œsophage, l'estomac, et du côlon. Il existe très peu de données publiées pour la localisation duodénale. Cette étude monocentrique avait pour but d'évaluer les résultats de cette technique dans une cohorte de 35 patients, le risque de complications hémorragiques et leurs facteurs prédictifs.
P95 Non Cf Bronchiectasis
Authors: C. Baggott, E. Harris, J. Suntharalingam, A. Malin
Journal: Thorax (2014)

<h3>Background</h3> Bronchiectasis is said to affect 100/100 000, however the true prevalence is probably significantly higher. Our previous BTS bronchiectasis audit identified that a significant service improvement was required. This led to the introduction of a home intravenous antibiotic pilot, a bronchiectasis working group, and enhanced teaching and information sharing with primary care. Despite this, these patients remain poorly served both in the community and hospital. From 2009–13 there were 330 bronchiectasis admissions to our hospital. A significant proportion of these could have been managed in the community, substantially reducing the cost to the NHS. <h3>Aims</h3> We are developing an integrated bronchiectasis service between hospital and community. The vision has been to create a primary and secondary care interface, utilising the usual multi-disciplinary team plus psychology and dietetic support, a ‘<i>hospital without walls’</i> model and an online, multi-faceted communication tool. <h3>Outputs</h3> We provided <i>Pseudomonas</i> eradication therapy (previously published – White <i>et al</i>. 2012) and have piloted a home intravenous antibiotic service providing treatment for exacerbations which saved 497 hospital bed days in 2013. Twenty one patients received 37 home intravenous courses; of these, 17 courses were self-administered. Overall we reduced annual bronchiectasis admissions by 30% when comparing with 2011–12, equivalent to 23 fewer admissions over the year. We have developed an online database and clinical record tool which can be shared and updated by hospital and community alike. As well as allowing rapid communication, the database shows trend analysis, logging of microbiology/antibiotic use and is a valuable audit and research resource. We held a recent workshop comprising CCG, community partners and hospital stakeholders. We developed a new dynamic care pathway showing a combined “community/hospital hub” which will work with partners in primary and secondary care (Figure 1). We propose that such shared care working represents a useful model for broad application elsewhere.
Abstract 3888: Next generation cell engineering: the Jump-In cell engineering platform
Authors: Kurt W. Vogel
Journal: Cancer Research (2012)

Abstract The stable introduction of genetic material into cell lines has become one of the most widely used methods since its introduction more than 3 decades ago. Cell engineering has become an indispensable tool for a wide range of areas in Life Science, such as cell biology, physiology, In-vivo modeling, drug discovery and bioproduction. However, most cell engineering strategies currently employed for the generation of stable cell lines require substantial commitment in respect to time, FTE hours and resources. In addition, most cell lines are generated by relying on random integration of genetic material into the genome, which typically results in a wide range of expression levels within a cell population. For these reasons the frequent generation of engineered cell lines is in general cumbersome and unpredictable. Life Technologies recently developed and validated the Jump-In™ cell engineering platform, a novel gene targeting technology based on R4 integrase mediated site specific homologous recombination. This technology allows the targeted integration of genetic material into a specific pre-engineered site, which by design reduces the effort required for generation of stable cell lines compared to standard methods. Isogenic expression from a defined genomic locus provides the ideal solution for comparative analysis of gene families, isotypes or orthologs. This technology was applied to two widely used cellular backgrounds, HEK293 and CHO-K1. We used these cellular backgrounds as case studies to demonstrate how this technology can be applied to rapidly generate engineered cell lines for a variety of cell based assay applications, including reporter assays for pathway analysis, GPCR activity assays and analysis of protein post-translational modifications. In summary, Jump-In™ technology enables researchers to accelerate the development of cellular assays by greatly reducing the amount of time and resources required for the upfront cell engineering process. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3888. doi:1538-7445.AM2012-3888
TCTAP A-086 Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the Multifunction-CardioGram (MCG), to Coronary Angiography or MDCT, SPECT for the Detection of Revalent Coronary Artery Stenosis
Authors: Makoto Kadotani
Journal: Journal of the American College of Cardiology (2015)
Idiopathic Disseminated Focal Arteritis
Authors: Edgar A. Hines
Journal: JAMA (1960)

The medical histories of three men, aged 17, 36, and 44, presented an unusual form of arteritis. There were symptoms and signs of a generalized systemic disease, with cutaneous erythematous nodules and multiple pulsating tumors of large and medium-sized arteries. The cutaneous nodules on histopathologic examination resembled those of erythema nodosum, and the pulsating tumors were false aneurysms probably arising from a tear in the artery in an area of focal arteritis. The false aneurysms had developed during intervals of weeks or months. These cases represent a unique and probably rare clinical entity.
THU0326 Relationship between Regular Exercise, Functional Capacity and Quality of Life in Pacients with Fibromyalgia
Authors: S. Alina
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Fibromyalgia (FM) is a chronic musculoskeletal disorder characterized by generalized muscular pain accompanied by fatigue and tenderness at specific anatomical sites called tender points; additionally other associated symptoms include altered sleep, with cognitive and emotional disturbances as anxiety and depression, leading to disability and poor quality of life. Various exercise programs are accepted as treatment for FM, with complex actions on different muscular groups, leading to pain decrease, postural equilibrium and mobility improvement. <h3>Objectives</h3> Verify the relationship between functional capacity as well as a self report of quality of life with the practice of regular exercise in patients with FM <h3>Methods</h3> A total of 320 patients diagnosed with FM were recruited between May of 2012 and December of 2013 from Rehabilitation outpacients clinics in Romania. The patients were divided in two groups. Group1 included patients who reported practice of regular exercise (n=170; 50,7±13,04 years), while group 2 included those who reported non-practice of regular exercise (n=150; 49±20 years). Functional capacity and quality of life was measured using the Health Assessment Questionnaire-HAQ and Short Form – SF-36 <h3>Results</h3> Significant differences in the quality of life and functional capacity were observed when group 1 was compared with group 2. A lower score of functional capacity (HAQ) was found in group 2when compared with the group 1 (p=0,0006). Higher values were obtained for the group 1 when compared with group 2 for the following domains of SF-36: physical functioning (p&lt;0,001) and bodily pain (p&lt;0.05). No significant differences were found for role physical, general health, vitality, social functioning, role emotion and mental health domains. <h3>Conclusions</h3> Regular exercise showed a positive impact in the functional capacity and quality of life in patients with FM. <h3>References</h3> Ann Rheum Dis 2011;70(Suppl 3):765 <h3>Disclosure of Interest</h3> : None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.1615
Abstract LB-293: High-throughput proteomic analysis identifies protein s as a modulator of high grade and castrate-resistant prostate cancer
Authors: Punit Saraon, Keith Jarvi, Eleftherios P. Diamandis
Journal: Cancer Research (2012)

Abstract High-Throughput Proteomic Analysis Identifies Protein S as a Potential Modulator of Castrate-Resistant Prostate Cancer Punit Saraon, Daniela Cretu, Colm Morrissey, Keith Jarvi, Eleftherios P. Diamandis Androgen-deprivation remains the principal therapy for advanced and metastatic prostate cancers. However, some cancer cells can survive this treatment and transform themselves to a more aggressive androgen-independent prostate cancer (AIPC). An understanding of the molecular alterations that occur during the progression to androgen-independence is an integral step to generate effective targeted therapies. Using Mass Spectrometry, we compared the proteomes of androgen independent cell lines (PC3, DU145, PPC1, LNCaP-SF, 22Rv1) to androgen-dependent (LNCaP, VCaP) and normal prostate epithelial (RWPE) cell lines. We identified more than 100 proteins that were differentially secreted in the androgen independent cell lines, based on spectral counts. Of these, Protein S (PROS1) was elevated in the secretomes of all of the AIPC cell lines, with no detectable secretions in normal and androgen dependent cell lines. Using qPCR, we observed significantly higher tissue expression levels of PROS1 in prostate cancer samples (p&amp;lt;0.05), further indicating its importance in prostate cancer progression. Similarly, immunohistochemistry analysis revealed elevation of PROS1 during high grade prostate cancer (Gleason ≥ 8), and further elevation in castrate-resistant metastatic prostate cancer lesions. We also observed its elevation in high grade prostate cancer seminal plasma samples (P&amp;lt;0.05). To understand the functional role of PROS1 with respect to prostate cancer progression, we generated stable PROS1 knock-downs in DU145 cells, and performed cell migration and viability assays. In-vitro scratch assays measuring cell migration and proliferation revealed that PROS1 enhanced growth of prostate cancer cells, as there was significantly reduced wound closure in shPROS1 DU145 cells compared to scrambled control cells (p&amp;lt;0.05). In addition, cell viability assays showed that shPROS1 cells had reduced survival rates compared to scrambled cells when treated with the chemotherapeutic agents, docetaxel and paclitaxel, indicating its role as a potential anti-apoptotic factor. Taken together, our preliminary results show that PROS1 is elevated during high grade and castrate-resistant prostate cancer, and promotes prostate cancer migration and cell survival. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-293. doi:1538-7445.AM2012-LB-293
Abstract 3092: FBW7 targets hypoxia inducible factor-1α (HIF-1α) for proteasomal degradation during hypoxia
Authors: Jessica Cassavaugh, Sarah Hale, Cheung Wong, Karen M. Lounsbury
Journal: Cancer Research (2011)

Abstract Hypoxia Inducible Factor-1α (HIF-1α) mediates expression of genes associated with endothelial cell-mediated angiogenesis and is associated with poor outcomes in a variety of cancers. In normoxia, HIF-1α is ubiquitinated and degraded through interactions with the E3 ubiqutin ligase, von Hippel-Lindau (vHL); however, little is known about the negative regulation of HIF-1α in hypoxia. FBW7, an E3 ubiquitin ligase, has been shown to interact with several transcription factors including those phosphorylated by glycogen synthase kinase 3β (GSK3β). The current study tested the hypothesis that phosphorylation of HIF-1α by GSK3β increases the FBW7-mediated ubiquitination and degradation of HIF-1α, thereby resulting in suppression of the hypoxia-mediated angiogenic response in SKOV-3 ovarian cancer cells. HIF-1α protein and VEGF transcript levels in hypoxia were increased when GSK3β activity was inhibited and were reduced by expression of constitutively active GSK3β (GSK3S9A). Additionally, expression of GSK3S9A increased HIF-1α ubiquitination. Conditioned media from SKOV-3 cells expressing shRNA against GSK3β (shGSK3) had an enhanced effect on endothelial tube formation in a Matrigel matrix, suggesting that GSK3β exerts an inhibitory effect on hypoxia-mediated angiogenesis. Overexpression of the β and γ isoforms of FBW7 decreased HIF-1α stabilization, and HIF-1α interacts with FBW7 by co-immunoprecipitation. Furthermore, knockdown of FBW7 using siRNA resulted in increased HIF-1α levels in hypoxia. These data suggest a new mechanism for negative regulation of HIF-1α during hypoxia that utilizes phosphorylation by GSK3β and interaction with FBW7 leading to ubiquitination and proteasomal degradation. Results of this study better define the signaling pathways necessary for HIF-1α-mediated signaling and may identify new targets that mediate angiogenesis in disease. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3092. doi:10.1158/1538-7445.AM2011-3092
P5-09-05: Non-Palpable Lesions Findings in a Breast Cancer Opportunistic Screening in South Brazil.
Authors: R Rockenbach, RL Rombaldi, M Caleffi
Journal: Cancer Research (2011)

Abstract Introduction: Today breast cancer is a major public health challenge in developing countries with increasing mortality rates due to the lack of early detection programs and access to care. Mammography has been considered the most sensitive test for breast non-palpable lesions. The Brazilian National policy recommends mammography from age 50 and every two years. This study investigates only radiological findings in a set of women seeking for long term breast care surveillance. Objectives: To evaluate patients characteristics of cases with non-palpable breast lesions. Material and method: A prospective and transversal study was undertaken between March,2009 and April,2010 on 110 women with non-palpable breast lesions, diagnosed by mammography or ultrasound. Histo-pathological evaluation was performed in core biopsy specimen. The following variables were studied: pregnancy, parity, menarche, menopause, hormone replacement therapy, breast lesion location, tumor size, histopathology findings, mammographic and ultrasound classification — BI-Rads. Results: 23,7% (n=26/110) out of 110 women were diagnosed with breast cancer and 76,3% (n=84/110) were diagnosed with benign mammary pathology. Mammography, according BIRads classification, strongly suggested (p&amp;lt;0,000) the presence of benign and malign pathologies. In the breast cancer group, 14 were diagnosed with stage 1 (TNM), compared to the official data from INCA where stage I is 10% of the cases. Conclusion: Mammographic screening reduces mortality by 30% after 50 years of age and also provides important benefit (20%) in younger women (40-49). Our study showed a cancer detection rate of 25,7% and 34,6%, respectively, demonstrating the importance of screening also in younger ages. Overall, the rate of biopsy positive was very satisfactory (1 out of 4 cases). Among the studied non-palpable breast lesions, our findings resulted in 53.8% stage I breast cancers (TNM) which reinforces the urgent need of a structured screening program in Brazil to save more lives. Financial support: Avon Institute. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P5-09-05.
THE USE OF GROUP PSYCHOTHERAPY IN POSTHOSPITALIZATION TREATMENT
Authors: DAVID BLAU, JOAN J. ZILBACH
Journal: American Journal of Psychiatry (1954)

Eight patients on trial visit were treated for 1 year in group psychotherapy. During this time none of the members was rehospitalized and all were discharged, though many had had numerous previous admissions and had been unable to remain out of the hospital during the 1-year trial visit period. The group therapy meetings have been described. In these meetings certain phenomena were outstanding. We have described the early group attitude, and the subsequent redefinition of the leader's role and the role of the group. This structuring of the group was very important in facilitating free expression of feelings by the group members. It was clear that the members expected retaliation to follow the expression of hostile feelings. We have described the group sessions in which this exploration and testing was done. As a result of being able to express more freely their feelings in the group, the members could examine more clearly their relationships with key figures in their lives. We have also compared the pre- and posthospitalization adjustment of the group members. We find that there have been significant improvements in socialization, marital and job adjustment. Improved socialization should be particularly noted since some of these patients had a history of only the most meager experience in interpersonal relationships. Perhaps this was possible in the controlled situation of the group, because there they learned about some of the real feelings of themselves and of other people. This seems to have facilitated the development of more meaningful relationships with people in general. The evaluation of clinical proceedings is always very difficult. However, the promising results of this preliminary study open the possibility of working through some of the difficulties that the psychotic patient faces when leaving the hospital.
ET-60 * ENHANCED THERAPEUTIC EFFICACY OF PRODRUG ACTIVATOR GENE THERAPY WITH A NON-LYTIC RETROVIRAL REPLICATING VECTOR (TOCA 511) COMBINED WITH RADIATION THERAPY IN EXPERIMENTAL GLIOMA
Authors: M. Takahashi, G. Valdes, A. Inagaki, S. Kamijima, K. Hiraoka, E. Micewicz, W. H. McBride, K. S. Iwamoto, K. Hermann, S. Collins, Q. Tam, H. E. Gruber, J. M. Robbins, D. J. Jolly, N. Kasahara
Journal: Neuro-Oncology (2014)

A tumor-selective non-lytic retroviral replicating vector (RRV), Toca 511 (vocimagene amiretrorepvec), is being investigated in clinical trials in patients with recurrent high grade glioma (rHGG) (www.clinicaltrials.gov NCT01156584, NCT01470794, NCT01985256). Toca 511 encodes a modified yeast cytosine deaminase (CD), which converts oral prodrug 5-FC into the anticancer drug 5-fluorouracil (5-FU) within infected tumor cells. Since 5-FU is a radiosensitizer, we investigated in preclinical models the combination of Toca 511, 5-FC and radiation for treatment of human HGG. U87 and radioresistent U87EGFRvIII cells were infected in vitro with Toca 511 and subsequently irradiated with 0, 3, 6 or 9 Gy. In non-irradiated and irradiated cells, RRV infected >95% of cells by day 21 showing that radiation does not perturb viral spread. In vitro clonogenic survival assays showed significant radiosensitization with 5-FC in RRV-infected U87EGFRvIII cells. For in vivo survival studies, U87EGFRvIII cells (5 x 104) were stereotactically implanted into athymic mouse brain and Toca 511 (105 Transducing Units) was injected intratumorally 4 days later. Mice were treated with a single cycle of intraperitoneal PBS or 5-FC (500 mg/kg) for 5 days from day 10 to 14, with or without irradiation (2 Gy/fr/day; total 10 Gy, or 4 Gy/fr/day; total 20 Gy). Mice treated with 20 Gy and a single cycle of 5-FC showed significantly longer survival compared with the other two groups (p<0.0001) and median survival was >75 days. Next, mice were treated with multiple cycles of PBS or 5-FC (intraperitoneal, 5 days every 2 weeks, 5 cycles) with or without irradiation at the lower dose (total 10 Gy). Mice treated with 10 Gy and 5 cycles of 5-FC showed significantly longer survival (p<0.0001) and median survival was >89 days. These efficacy data support clinical investigation of Toca 511 and 5-FC in combination with radiation in the first-line setting for patients with HGG.
Gallbladder Visualization on RBC Scintigraphy
Authors: SERGI VIDAL-SICART, FRANCISCO LOMEÑA, FRANCISCO JAVIER SETOAIN, RAMÓN HERRANZ
Journal: Clinical Nuclear Medicine (1996)

The gallbladder was observed 24 hours after labeled RBC reinjection on a Tc-99m RBC gastrointestinal bleeding site imaging study. In some cases, the presence of the tracer in the gallbladder could represent hematobilia. In other cases, it may also be due to Tc-99m labeled to the porphyrin portion of hemoglobin.
Feasibility and Outcome of a Brief Cognitive Behaviour Therapy Family Intervention for Patients with Obsessive-Compulsive Disorder: A Pilot Study
Authors: Karin C.P. Remmerswaal, Neeltje M. Batelaan, Johannes H. Smit, Patricia van Oppen, Anton J.L.M. van Balkom
Journal: Psychotherapy and Psychosomatics (2016)
PWE-065 Should focal colonic activity found on<sup>18</sup>FDG PET CT be followed-up with endoscopic evaluation?
Authors: C L Y Wong, W J Jafar, B Rajashanker, R C Prudham, M Prescott, P A Hulse, J K Limdi
Journal: Gut (2012)

<h3>Introduction</h3> PET CT is a non-invasive imaging modality that is widely used in the imaging work up of malignant disease. It has been postulated that due to <sup>18</sup>FDG accumulation in adenomatous polyps, PET CT using <sup>18</sup>FDG may detect early premalignant colonic lesions. The aim of our study was to assess the performance of <sup>18</sup>FDG PET CT in the detection of significant colonic lesions namely adenomas or villous adenomas &gt;1 cm in size and to assess whether endoscopy in all patients with focal FDG colonic uptake on PET CT is justified by the detection rate. <h3>Methods</h3> A retrospective review of patients who had <sup>18</sup>FDG PET CT at the Pennine Acute Hospitals NHS Trust, Manchester as part of their staging for various cancers between February 2007 and October 2011 was conducted by a review of the reports of the examinations. <h3>Results</h3> 1553 patients between the ages of 17 and 93 years (median age 55 years) had <sup>18</sup>FDG PET CT performed during this period. Increased focal FDG uptake in the colon was detected in 179 of 1553 patients studied (11.5%). Endoscopic evaluation was performed in 71 (39.7%) of these patients, flexible sigmoidoscopy in 25 (35.2%) and colonoscopy in 46 (64.8%). Non-malignant pathology was found in 45 of 179 patients (25.1%); 17 (9.5%) were tubulovillous adenomas; 6 (3.4%) had villous adenomas; 9 (5.0%) were tubular adenomas; 4 (2.2%) hyperplastic polyps; 11 (6.1%) were normal colonic tissue and 1 (0.6%) was active inflammatory bowel disease. From the patients with increased <sup>18</sup>FDG uptake in the colon, 19 (10.6%) were subsequently found to have adenocarcinoma on histology. This represents 26.8% of the 71 patients who received endoscopic evaluation. <h3>Conclusion</h3> The presence of focal colonic <sup>18</sup>FDG uptake on PET CT scan justifies endoscopic evaluation in all patients where treatment would be clinically appropriate. <h3>Competing interests</h3> None declared.