tbuckley/cabot-search huggingface dataset:
Abstract 77: Study of the effect of MAPRE2 on human lung adenocarcinoma cell migration and invasion
Authors: Yi-Hua Lai, Ching-Hsien Chen, Meng-Feng Tsai, Huei-Wen Chen, Kang-Yi Su, Ang Yuan, Jeremy J.W. Chen
Journal: Cancer Research (2012)

Abstract Lung cancer is the most malignant disease which makes lots of people dead worldwide. More and more evidences showed that cancers might arise from chromosome alteration, including amplification or deletion. Our previous study integrating microarray-based comparative genomic hybridization and affymetrix gene expression profiles identified a potential candidate gene, MAPRE2, in a lung cancer cell line model with different invasion capability. MAPRE2 (Microtubule-associated protein RP/EB family member 2) is usually located on microtubule and interactes with tumor suppress gene APC (adenomatous polyposis coli). MAPRE2 might be involved in microtubule polymerization, cell migration and tumorigenesis of colorectal cancers, but its function is unclear. The purpose of this study is to investigate the effects of MAPRE2 on cancer cell function. The results of real-time PCR and western blotting showed that DNA copy number, RNA and protein level of MAPRE2 were higher in low invasive lung cancer cells than in highly invasive cells. Immunofluorescence assay indicated that the distribution of MAPRE2 protein was predominant in cytoplasm. Furthermore, overexpression of MAPRE2 could inhibit cancer cell proliferation, anchorage- dependent and -independent growth, cell motility, invasion ability in vitro and tumor growth and metastasis in vivo. On the contrary, silencing MAPRE2 enhanced lung cancer cell proliferation and invasion ability. Consider the whole map of MAPRE2-regulated signalling, transcriptomic analysis was performed and the results showed that MAPRE2 could affect 1039 genes (ANOVA, FDR&amp;lt; 0.05) which are dominantly involved in cell cycle and adhesion-related biological signalling, such as MAPK, focal adhesion and tight junction pathways. Our results suggested that MAPRE2 might play a significant role in MAPRE2-mediated pathways to suppress tumorigenesis and metastasis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 77. doi:1538-7445.AM2012-77
Abstract 1555: Immune suppression of regulatory T cells and M2 macrophage in breast cancer patients
Authors: Shigeru Imoto, Noriko Sakemura, Hiroki Ito, Kentaro Imi, Hirotsugu Isaka, Eri Tazaki, Hiroshi Kanma, Noriaki Wada, Tetsuya Nakatsura
Journal: Cancer Research (2011)

Abstract Background: Recent multi-disciplinary treatments of breast cancer (BC) could decrease the mortality rate, but successful immune therapy remains uncertain. To explore new strategy of immune therapy of BC, we are investigating about host-tumor immune response in BC patients. Materials and methods: Tumor tissue specimens and peripheral blood mononuclear cells (PBMC) were obtained from early and advanced BC patients. PBMC were also collected from healthy volunteers. Regulatory T (Treg) cells were examined by counting CD4+CD25highCD127low/-cells in PBMC with flow cytometry analysis. Immunohistochemical evaluation of tumor specimens was performed with monoclonal antibodies of HLA-ABC and DR, CD56, CD68, CD83, and CD163. The number of stained cells was analyzed using a semiquantitative ordinal scale ranging from 0 to 3 (0, +/-, ++, +++). Results: HLA type I and type II were stained negative in 44% and 82% of 50 BC cases. When host-tumor immune response were compared between 38 early BC cases and 12 advanced BC cases, numbers of CD68-positive cells significantly increased in peripheral tumor tissues of advanced BC cases than in those of early BC cases (92% versus 53%). CD163-positive tumor cells were also detected more frequently in advanced BC cases than in early BC cases (75% versus 16%). There were no significant differences of distribution of CD4, CD8, CD56, and CD83-positive cells in early and advanced BC cases. Treg cells in PBMC significantly increased in percentage of the population in 46 BC patients than in 16 healthy volunteers (3.8% versus 2.1% of CD4-positive cells at mean value). Interestingly, Treg cells decreased in percentage of the population in postoperative BC patients. Conclusions: Our results suggest that Treg cells render BC patients under immune suppression and M2 macrophage plays an important role of tumor progression. Targeted therapy against M2 macrophage traits may be a promising strategy of breast cancer. Host-tumor immune response will be reported for BC patients treated with primary chemotherapy or radiofrequency ablation therapy in comparison with those who underwent breast surgery first. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1555. doi:10.1158/1538-7445.AM2011-1555
Abstract 1671: A simple HPLC method to measure plasma mitotane and its two main metabolites in adrenocortical cancer patients
Authors: Madhu B. Garg, Jennette A. Sakoff, Stephen P. Ackland
Journal: Cancer Research (2010)

Abstract Adrenocortical carcinoma (ACC) is a rare, aggressive tumour which is often detected in advanced stage. Mitotane (o,p’-DDD) is the drug of choice for unresectable, recurrent and metastatic ACC. Intraadrenal metabolic transformation is essential for its therapeutic effects but the metabolite responsible is unknown (o,p’-DDE or o,p’-DDA). Benefit depends on maintenance of adequate plasma drug levels &amp;gt;14μg/ml and toxicity occurs at &amp;gt;20μg/ml. The relationship between dose administered and steady state plasma level is variable, with up to 5-10 fold inter-patient variation. Plasma level monitoring during treatment allows more rapid and consistent attainment of levels between 14-20μg/ml. Measurement of o,p’-DDD and metabolites o,p'-DDE and o,p’-DDA could provide a better understanding of mitotane pharmacokinetics and pharmacodynamics. We have developed and validated a simple HPLC method to measure plasma mitotane and its two principal metabolites using an internal standard (p,p’-DDD), which involves an isocratic elution and separation through a reversed phase phenyl HPLC column with UV detection (λ 226nm). All these compounds are well separated from each other and from other interfering peaks with a total run time of 21min. The method has been evaluated using 63 plasma samples from 11 patients on mitotane therapy, median age 53 years (range13-73) with start dose of 1-4.5 gm/day. Trough o,p’-DDD levels ranged between 0.9-37.7μg/ml, o,p'-DDE levels 0-4.4μg/ml and o,p’-DDA levels 8.5-90.8μg/ml indicating o,p’-DDA levels in patient plasma are about 1.2-18.1 times higher than the parent compound. 37 samples with o,p’-DDD levels &amp;lt;14μg/ml were recommended to steadily increase dose up to 12 gm/day to achieve target level and then continue at that dose. 10 samples with o,p’-DDD levels &amp;gt;20μg/ml were recommended to decrease dose. Correlation with clinical data would allow us to confirm a relationship between plasma level of o,p’-DDD, o,p'-DDE and o,p’-DDA and clinical outcome, including tumour response or stablisation and toxicity. We aim to establish a therapeutic drug monitoring facility for assessment of plasma mitotane and metabolite levels in patients in the Asia Pacific region. A result and dosing recommendation could be available within 7 days of receipt of sample. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1671.
Case 23-2011
Authors: William H. Barth, Christopher J. Kwolek, Joshua L. Abrams, Jeffrey L. Ecker, Drucilla J. Roberts
Journal: New England Journal of Medicine (2011)

A 40-year-old woman was seen by the maternal–fetal medicine service of this hospital at 22 weeks 2 days' gestation because of placenta previa and placenta accreta. She declined transfusions of blood products because of her religious beliefs. Management decisions were made.
Abstract 3011: Evaluation of a high throughput platform for chromatin immunoprecipitation
Authors: Zirong Li
Journal: Cancer Research (2011)

Abstract Expression of eukaryotic genes during development requires complex spatial-temporal regulation. This complex regulation is often achieved through the coordinated interaction of transcription regulatory elements in the promoters of the target genes. The identification and mapping of regulatory elements in genome scale is crucial to understand how gene expression is regulated Chromatin immunoprecipitation is a standard method for assessing the occupancy of DNA binding proteins in vivo in their native chromatin context using antibodies. However, standard chromatin immunoprecipitation procedure is time consuming, labor intensive and not suited for analyzing many samples simultaneously. Recently, we have developed a simple chromatin immunoprecipitation protocol, which utilizes high capacity protein A/G coated magnetic beads and 96 well plate. This protocol requires fewer steps, little hands-on time and is compatible with multi-channel pipetting and liquid handling robot. This protocol allows genome wide mapping of transcription regulatory network in a high throughput manner. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3011. doi:10.1158/1538-7445.AM2011-3011
Abstract 86: Role of autophagy in lymphoma treatment: Autophagy manipulation in lymphoma therapeutic cell killing
Authors: Jacob M. Gump
Journal: Cancer Research (2010)

Abstract Manipulation of autophagy to augment cancer treatment is an area of active research. We sought to determine the role of autophagy in lymphoma treatment with cytotoxic chemotherapeutics alone and in combination with both genetic and pharmacologic manipulation (activation and inhibition) of autophagy. We found that cellular responses to certain chemotherapeutic agents are altered by autophagic manipulation whereas others are not. Using single cell microscopic assays and flow cytometry we have found that manipulation of autophagy has an effect on a subset of lymphoma cells in culture altering their response to cell death stimuli. The overall magnitude, kinetics and mechanistic aspects of apoptosis are altered in this subset of cells. This finding that there are differences in the response to autophagy manipulation between cancer cells in a population has implications for the treatment of lymphoma and other cancers. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 86.
M3 Evaluation of Physical Activity in patients with Severe Asthma–Are we getting it right?: Abstract M3.
Authors: LJF Grillo, S Greenwood, J Hull, A Menzies Gow
Journal: Thorax (2013)

<h3>Introduction</h3> Of the 5 million patients who receive medication for asthma approximately 5% have severe or difficult to treat disease. A key problem for patients with severe asthma is impaired exercise capacity and often reported as a trigger for symptoms. In COPD it is recognised that reduced Physical Activity (PA) levels can be linked with poorer morbidity and increased exacerbations. In Severe Asthma (SA) there has to date been no formal evaluation of self reported PA levels in comparison to objective measurements and Quality of Life (QoL). <h3>Objectives</h3> To evaluate self reported PA in a population of patients with SA compared to 6 Minute Walk Test (6MWT) results. To compare PA levels with others measures of disease including lung function and QoL measurements (Short Form 36 or SF36) <h3>Methods</h3> 35 patients (12 male:17 Female) with SA completed the Dukes Activity Status Index (DASI) which gives an estimated maximum metabolic equivalent (METs) as an estimation of self reported physical function. QoL was measured with the SF36 where Physical component scores (PCS) and Mental Component Scores (MCS) were collected. Objective measures of Forced Expiratory Volume in 1 second percent predicted (FEV<sub>1 </sub>%) and 6MWT distance (metres) were also collected. <h3>Results</h3> The 6MWT had medium correlations with SF36 MCS (r<sub>s</sub> = 0.372, p &gt; 0.03) and SF36 PCS (r<sub>s</sub> = 0.480, p &gt; 0.004) and a strong correlation with the DASI (r<sub>s</sub> = 0.604, p &gt; 0.0001). Moreover the Physical function component of SF36 (SF36PF) also had a strong correlation with 6MWT (r<sub>s</sub> = 0.541, p &gt; 0.001). FEV<sub>1 </sub>showed a medium correlation with 6MWT (r<sub>s</sub> = 0.413, p &gt; 0.19). <h3>Conclusion</h3> Patients appear to estimate PA levels accurately with the DASI, with higher scores of estimated METs correlating with increased 6MWT distance. QoL appears higher in those patients who achieve a greater distance in their 6MWT, especially in relation to their physical function. FEV<sub>1 </sub>preservation also appears to correlate with improved 6MWT distances. Further investigation of PA in this popoulation is warranted.
Total Avulsion of the Scalp
Authors: Varaztad H. Kazanjian, Aram Roopenian
Journal: New England Journal of Medicine (1950)

MANY cases of total avulsion of the scalp have been reported in the medical literature, and the treatment has been extremely varied. Formerly, these large wounds were allowed to granulate and gradually to epithelialize spontaneously from the edges. The wounds became severely infected, and the healing period was greatly protracted. Many of the patients treated in this fashion became debilitated from chronic sepsis; some suffered osteomyelitis of the calvarium, and others intracranial complications by extension of the infection through diploetic channels. The end result in those who survived was poor, the thin scar epithelium constantly breaking down, and the contracting . . .
AB1272 Subacromial steroid injection do not increase the rate of full-thickness rotator cuff tear
Authors: J. Ramírez, I. Pomés, S. Cabrera, M.E. Gόmez, J. Inciarte, V. Rosario, J. Pomés, R. Sanmartí, J. Cañete
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Up to 35% of patients with shoulder pain have a full-thickness rotator cuff tear. It has been proven a deleterious effect of corticosteroid on collagen. However, the influence of corticosteroid injection in the incidence of rotator cuff tears is unknown. <h3>Objectives</h3> The aim of this study was to evaluate the risk of corticosteroid injection in the incidence of full-thickness rotator cuff tear. Secondary outcomes were the incidence of full-thickness rotator cuff tear between patients with partial-thickness rotator cuff tear and the improvement in shoulder pain and function. <h3>Methods</h3> Prospective, open-label study. Patients with unilateral painful shoulder without previous local steroid injection were recluted. Clinical and ultrasound assessment were practiced in the first (day 0) and the last visit (day 90). Patients with full-thickness rotator cuff tear were excluded. Patients received a standard subacromial infiltration of 1 cc of triamcinolona depot. 1cc of mepivacaina was injected in 7 patients for clinical decision (mainly poorly controlled diabetes). A radiologist and a rheumatologist experienced in musculoeskeletical ultrasound performed the ultrasound scans and clinical examination, respectively, of shoulder pain. <h3>Results</h3> 89 patients with shoulder pain were evaluated. 47 (52.8%) had full-thickness rotator cuff tear in the first visit and were excluded. 42 patients completed the study (29 women and 13 men, the mean age of patients was 61.1 years-old). 35 received an injection of 1 cc of triamcinolone depot and 7 were infiltrated with mepivacaine (2 patients for steroid allergy and 5 patients with poorly controlled diabetes). At the end of the study, 7 full-thickness rotator cuff tears were found. 1/7 (14.2%) in the mepivacaine group and 6/35 (17.1%) in the triamcinolone depot group. Among 18 patients who had a partial-thickness rotator cuff tear in the first visit, 6 suffered a complete tear of the rotator cuff after 3 months (1/4 in the mepivacaine group and 5/14 in the triamcinolone depot group). 4/7 patients (57.1%) had a great improvement in shoulder pain (more than 80% in VAS score) in the mepivacaine group, while 14/35 (40%) had it in the triamcinolone depot group. Only one patient worsened the symptoms, because of a new full-thickness rotator cuff tear. 39 patients (92.8%) recovered the normal range of movement after 3 months. <h3>Conclusions</h3> Subacromial steroid injection did not increase the rate of full-thickness rotator cuff tear expected in a population with shoulder pain. More than half patients with shoulder pain have a full-thickness rotator cuff tear in the first visit. <h3>Disclosure of Interest</h3> None Declared
PSA Becoming Important Tool in Prostate Cancer
Authors: N. Volkers
Journal: JNCI Journal of the National Cancer Institute (1992)

Journal Article PSA Becoming Important Tool in Prostate Cancer Get access Nancy Volkers Nancy Volkers Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 84, Issue 4, 19 February 1992, Pages 221–222, https://doi.org/10.1093/jnci/84.4.221 Published: 19 February 1992
Abstract 704: Development of therapeutic antibodies targeting Notch3 in lung cancer
Authors: Luping Lin, Raymond Mernaugh, Robert Carnahan, David P. Carbone, Thao P. Dang
Journal: Cancer Research (2010)

Abstract The Notch receptors are essential for both normal development and tumorigenesis in many human cancers. Notch3 is expressed in 40% of all lung cancers. Inhibiting this pathway results in reduced tumor growth in lung in vitro as well as in vivo. Thus, this pathway represents a potentially important target for therapeutic development. Here we report the early results of a strategy to inhibiting Notch3 signaling through the development of Notch3 monoclonal antibodies. Using a Notch3 peptide library, we discovered two regions of the extracellular domain, believed to be the binding regions for the Notch3 ligand, Jagged1. The recombinant proteins representing these two regions, respectively, were used as antigens to immunize mice. We demonstrated that antisera from these mice immunized with portions of the receptor extracellular domain can inhibit Notch3 activation. Further developments of hybridoma clones were screened with ELISA, immunoprecipitation and their ability to inhibit Notch3 cleavage. 12 hybridoma clones were selected, and 4 out of 12 antibodies with IgG1 or IgM isotypes were found to specifically inhibit Notch3 activation but not Notch1. Further testing is on going to validate these findings as well as to determine affinity and anti-tumor activity of these antibodies. Development of monoclonal antibody to specifically inhibit Notch3 ligand binding regions could serve as a strategy for the development of future therapeutics for patients with lung cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 704.
SAT0360 Low bone mass is associated with carotid intima-media thickness and atherosclerotic plaque in postmenopausal women referred for osteoporosis screening
Authors: L.M. Muntean, S.P. Simon, A. Albu, L. Poanta, S. Rednic, D. Fodor
Journal: Annals of the Rheumatic Diseases (2012)

<h3>Background</h3> Low bone mineral density (BMD) has been associated with cardiovascular diseases (CVD) and markers of subclinical atherosclerosis, suggesting a shared pathophysiology. <h3>Objectives</h3> The objective of this study was to evaluate the relationships between bone mineral density (BMD) and intima-media thickness (IMT) of the common carotid artery and carotid atherosclerotic plaque in a cohort of postmenopausal women. <h3>Methods</h3> In this cross-sectional study were consecutively enrolled 184 postmenopausal women referred for osteoporosis screening by dual energy X-ray absorptiometry (DXA) scanning of the lumbar spine and hip. Mean common carotid IMT, as a marker of subclinical atherosclerosis, and the presence of atherosclerotic plaques were assessed by ultrasonography. <h3>Results</h3> In patients with total hip osteoporosis, mean IMT was significantly higher than in patients with osteopenia or normal BMD (both p&lt;0.05). There was no statistically significant difference in the mean values of IMT across the spine bone status (p=0.07). Sixty-three percent of patients with osteoporosis at the femoral neck had carotid artery plaque at one or more sites compared with 38% for women with normal BMD (p=0.04). There was no statistically significant trend in the proportion of atherosclerotic plaques across the spine and total hip bone status groups. A significant positive correlation was observed between mean IMT and BMD Z-score at the lumbar spine (rho =0.19, p=0.008). In regression analysis, femoral neck BMD, but not total hip or lumbar spine BMD, was negatively associated with mean carotid IMT (p=0.04). <h3>Conclusions</h3> Low bone mass in postmenopausal women is associated with increased carotid IMT and atherosclerotic plaque. Our results provide further evidence for a link between BMD and the risk of atherosclerotic cardiovascular diseases. <h3>References</h3> Den Uyl D, Nurmohamed MT, van Tuyl LH et al. (Sub)clinical cardiovascular disease is associated with increased bone loss and fracture risk; a systematic review of the association between cardiovascular disease and osteoporosis. Arthritis Res Ther 2011;13(1):R5. Sumino H, Ichikawa S, Kasama S, et al. Relationship between carotid atherosclerosis and lumbar spine bone mineral density in postmenopausal women. Hypertens Res 2008; 31:1191-97. Jorgensen L, Joakimsen O, Rosvold Berntsen GK, et al. Low bone mineral density is related to echogenic carotid artery plaques: a population-based study. Am J Epidemiol 2004;160:549-56. <h3>Disclosure of Interest</h3> None Declared
EFFECTIVE ORIFICE AREA BY CARDIAC MAGNETIC RESONANCE FOR THE FUNCTIONAL ASSESSMENT OF BIOPROSTHETIC AORTIC VALVES
Authors: Dimitrios Maragiannis, Matthew Jackson, Jose Flores-Arrendondo, Paulino Alvarez, Autry Kyle, William Zoghbi, Dipan Shah, Stephen Little, Stephen Little
Journal: Journal of the American College of Cardiology (2015)