tbuckley/cabot-search huggingface dataset:
Human Autologous Rosettes. II. Prognostic Significance of Variations in Autologous Rosette-Forming Cells in the Peripheral Blood of Cancer Patients2
Authors: Jean Caraux, Christian Thierry, Bernard Serrou
Journal: JNCI Journal of the National Cancer Institute (1979)

Journal Article Human Autologous Rosettes. II. Prognostic Significance of Variations in Autologous Rosette-Forming Cells in the Peripheral Blood of Cancer Patients Get access Jean Caraux, Jean Caraux 3Department of Tumor Immunopharmacology, Centre Paul La-marque, Hôpital St. Eloi, BP 5054, 34033 Montpellier Cedex, France. 4Recipient of the Fondation de la Vocation Award, 1976. Search for other works by this author on: Oxford Academic PubMed Google Scholar Christian Thierry, Christian Thierry 3Department of Tumor Immunopharmacology, Centre Paul La-marque, Hôpital St. Eloi, BP 5054, 34033 Montpellier Cedex, France. Search for other works by this author on: Oxford Academic PubMed Google Scholar Bernard Serrou Bernard Serrou 3Department of Tumor Immunopharmacology, Centre Paul La-marque, Hôpital St. Eloi, BP 5054, 34033 Montpellier Cedex, France. 5We thank Dr. C. L. Gauci for reviewing the manuscript and Mrs. A. Causse, Mrs. E. Ursule, and Mrs. H. Valles for expert technical assistance. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 63, Issue 3, September 1979, Pages 593–597, https://doi.org/10.1093/jnci/63.3.593 Published: 01 September 1979 Article history Received: 09 October 1978 Accepted: 04 January 1979 Published: 01 September 1979
STEM-17REPAIR OF RADIATION INJURY TO THE BRAIN USING HUMAN PLURIPOTENT STEM CELL DERIVED OLIGODENDROCYTE PROGENITORS
Authors: Jinghua Piao, Tamara Major, Viviane Tabar
Journal: Neuro-Oncology (2015)

Radiation therapy (RT) to the brain remains a powerful tool in the management of a wide range of cancers of the brain, head and neck regions and brain metastases. It is also used in prophylaxis in treatment regimens for leukemias and small cell cancers. There is an increasing awareness of significant and irreversible long-term side effects associated with RT, including cognitive decline and impairment of motor coordination. Our team and others have shown that depletion of oligodendrocyte progenitors (OPC) and demyelination are major pathological features that are particularly pronounced in younger individuals and severely limit therapeutic options. We have developed protocols for the derivation of oligodendrocyte progenitors from human ES or iPS cells. The cells can be expanded at large scale, and present a gene and protein profiles that are reminiscent of authentic telencephalic oligodendrocyte progenitors. These cells were capable of in vitro myelination in co-cultures with human neurons. We also established a rat model of fractionated brain radiation using a clinically relevant regimen. The rats present behavioral changes several months following radiation. OPCs were grafted in the corpus callosum bilaterally and the animals followed for several months. They were grafted with OPCsexpression and protein Here we tested whether human ESC-derived oligodendrocytes can functionally remyelinate the irradiated brain using a rat model. We demonstrate that the cells migrate throughout the major white matter tracts resulting in both structural and functional repair. Behavioral testing showed complete recovery of cognitive function while additional recovery from motor deficits required concomitant transplantation into the cerebellum. The ability to repair radiation-induced damage to the brain could dramatically improve the outlook for cancer survivors and enable more effective use of radiation therapies, especially in children.
Abstract 3857: Hedgehog signaling in primary prostate tumors and associated metastases
Authors: Jennifer Proctor, Veronica Travaglione, Igor Deyneko, Margaret Read, Rob Ross, Karen McGovern, Robert Vessella, John MacDougall
Journal: Cancer Research (2010)

Abstract Malignant activation of the Hedgehog (Hh) pathway is associated with multiple tumor types. The Hh pathway can promote growth of certain cancers via three modes of malignant activation: ligand-dependent signaling either to the tumor cell itself or between the tumor and its microenvironment, and ligand-independent signaling activated by genetic mutations. In certain cancers, tumor-derived Hh ligand signals in a paracrine manner to the stromal cells in the microenvironment that support tumor cell growth. Using a sensitive immunohistochemistry method for detecting Hh ligand, 83 percent of 105 human prostate tumor samples, including lymph and bone metastases from the same patients, were found to express high levels of Sonic Hedgehog (SHh) ligand. LuCaP 23.1 and LuCaP 35v xenografts were derived from lymph node metastasis from patients with prostate cancer. Paracrine Hh signaling is detected in both xenograft models, where the human tumor cells produce Hh ligand and the surrounding stromal cells respond with Hh pathway activation, as measured by Gli1 levels. IPI-926, a novel, small molecule Hh inhibitor that acts by blocking Smoothened, inhibits signaling between the tumor and stromal cells in both castration sensitive and castration resistant tumor models. Inhibition of Hh signaling with IPI-926 was then evaluated as a potential means to reduce growth of prostate cancer tumors and metastases. IPI-926 significantly delays relapse and slows the growth rate of castration resistant primary human prostate tumors post-docetaxel treatment. Additional preclinical studies with IPI-926 in combination with chemotherapy and targeted therapies in castration resistant tumor models are in progress. These pre-clinical results provide a rationale for testing Hh inhibitors in patients with castration resistant prostate cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3857.
AB0322 Evaluation of Vaccines in Rheumatoid Arthritis: Data from the COMORA Study
Authors: I. Hmamouchi, K. Winthrop, O. Launay, M. Dougados
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Rheumatoid Arthritis (RA) patients are at risk of suffering from infectious diseases including those of the respiratory tract. Such risk seems to be higher in RA than in the general population. Although vaccines have proven effective in RA patients, they are little used today. <h3>Objectives</h3> Our objectives were: (1) to describe the vaccines status of pneumococcus and influenza in patients with RA from different countries (2) to identify factors influencing pneumococcal or influenza vaccinations. <h3>Methods</h3> <i>Study design:</i> This was an ancillary study from COMORA cohort [1], including 3920 RA patients consecutively. <i>Data collected:</i> The demographic and disease characteristics data were collected. The time of the last pneumococcal vaccination and influenza was recorded using a questionnaire. <i>Data analysis:</i> Univariate and multivariate analysis was performed by SPSS 10.0. <h3>Results</h3> The rate of vaccination coverage was low with huge disparities between countries: 636 patients (17.2%) for pneumococcal vaccination (from 0% in Morocco to 56.5% in Egypt) and 938 patients (25.3%) for influenza vaccination (from 1% in Egypt to 66.2% in Japan). In multivariate analysis, factors associated with pneumococcal vaccination less than 5 years were: age&gt;65 years (OR=1.4, CI95% (1.1-1.8), p=0.003), absence of steroids (OR=1.4, CI95% (1.1-1.7), p=0.004), country (p&lt;0.0001), the high level of education (OR=1.6, CI95% (1.1-2.3), p=0.003), lung disease (OR=1.7, CI95% (1.1-2.5), p=0.006) and kidney disease (OR =2.0, CI 95% (1.1-3.5), p=0.01). In multivariate analysis, factors associated with influenza vaccination less than one year were: age&gt;65 years (OR=1.8, CI 95% (1.5-2.2), p&lt;0.0001), DAS28 &lt;3.2 (OR=1.2, CI95% (1.0-1.4), p=0.04), any treatment intake for another disease (OR=1.4, CI95% (1.1-1.7), p&lt;0.001), history of surgery related to RA (OR=1.5, CI95% (1.2-2.1), p&lt;0.0001) and country (p&lt;0.0001). <h3>Conclusions</h3> This study suggests a low coverage rates of vaccinations despite international recommendations with a huge disparity between countries. Identification of factors associated with the vaccine status might facilitate the improvement of such coverage <h3>References</h3> Dougados M, Soubrier M, Antunez A, et al. Prevalence of comorbidities in rheumatoid arthritis and evaluation of their monitoring: results of an international, cross-sectional study (COMORA). Ann Rheum Dis. 2014 Jan 1;73(1):62-8. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3339
A1.05 The mer tyrosine kinase receptor plays a protective role in joint inflammation by mediating efferocytosis
Authors: S Beermann, CEJ Waterborg, MB Bennink, G Lemke, MI Koenders, FAJ van de Loo
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background and objective</h3> Rheumatoid arthritis (RA) is a chronic autoimmune disease characterised by a hyper-inflammatory response in synovial joints. During arthritis, increased levels of pro-inflammatory mediators are present in the joint cavity attracting leukocytes of which many undergo apoptosis. One receptor involved in uptake of apoptotic cells is the Mer receptor tyrosine kinase. In addition, the Mer receptor is involved in an anti-inflammatory natural feedback mechanism of Toll-like receptor signalling. The main ligand for Mer is Protein S (<i>Pros1</i>), acting as a bridge between apoptotic cells and the Merreceptor on phagocytic cells. Our objective was to elucidate the role of Mer in experimental arthritis. <h3>Materials and methods</h3> KRN serum transfer arthritis was induced in either Mer-deficient mice or in wild type mice that overexpress <i>Pros1</i> in their knee joints. Mice were macroscopically scored and at day 7 or 14, respectively, knee joints were taken for histology. In collagen-induced arthritis (CIA), mice were intravenously injected with a commercially available Mer-specific antibodies or an adenovirus overexpressing <i>Pros1</i>. At day 30 or 31, respectively, knee joints were taken for histology&nbsp;and immunohistochemical staining of active Caspase-3. <h3>Results</h3> In Mer-deficient mice, induction of passive KRN arthritis enhanced the macroscopic score in both knee and ankle joints compared to wild type. In addition, increased cartilage and bone erosion was found in knee joints of Mer<sup>-/-</sup> mice. Intra-articular overexpression of <i>Pros1</i> before KRN serum injections markedly reduced arthritis severity in wild type mice. CIA mice treated with Mer-specific antibodies showed increased incidence and higher macroscopic knee score compared to those receiving an isotype control (IgG). Histological analysis showed significantly enhanced synovial inflammation and bone erosion. In addition, significantly more active Caspase-3 positive cells were present, a marker for apoptotic cells. This indicates reduced efferocytosis in the anti-Mer treated animals. In concordance with these data, CIA mice which systemically overexpressed Pros1 showed a significant reduction in knee inflammation. <h3>Conclusion</h3> These data indicate the importance of Mer in controlling the severity and resolution of inflammation in experimental arthritis. Our data suggest that promoting Mer-mediated uptake of apoptotic cells in the arthritic joint might be therapeutically beneficial.
Choroidal Metastasis of Papillary Thyroid Carcinoma Demonstrated on SPECT-CT
Authors: Nese Torun, Mehmet Reyhan, Ali Fuat Yapar, Muge Karatas
Journal: Clinical Nuclear Medicine (2016)

We report a 68-year-old woman with papillary thyroid carcinoma metastasizing to choroid. The choroid metastasis was diagnosed with SPECT-CT and then was treated with high-dose radioactive iodine therapy.
Electrocardiographic and Blood-Pressure Changes during and after Biliary-Tract Surgery
Authors: David Mendelsohn, Richard Monheit
Journal: New England Journal of Medicine (1956)

THE association of biliary-tract and coronary-artery disease has been noted clinically for many years.1 2 3 4 5 6 7 8 9 10 11 12 13 14 This relation has been investigated extensively in the laboratory. There have been repeated references to reflexes arising from the upper abdominal viscera and especially the biliary tract that could give rise to cardiac arrhythmias, a reduction in coronary blood flow and even catastrophe during surgery of the biliary tract.13 , 15 16 17 18 19 20 21 However, the work of several investigators failed to reveal any consistent significant electrocardiographic changes related to manipulation of this area.22 23 24 25 26 27 28 In the laboratory animal varied changes in coronary blood flow and in the electrocardiogram that were due . . .
Abstract 3083: Regulation of novel tumor suppressor gene FUS1 expression through 5’- and 3’-UTRs and in response to DNA damage in human lung cancer cells
Authors: Jing Lin, Kai Xu, Jack A. Roth, Lin Ji
Journal: Cancer Research (2010)

Abstract FUS1 is a novel tumor suppressor gene located in the human chromosome 3p21.3 region. FUS1 mRNA transcripts could be detected on Northern blots in both normal lung and some lung cancer cell lines, but no endogenous FUS1 protein could be detected in a majority of lung cancer cell lines. Loss or reduction of FUS1 expression was observed in all small cell lung cancer and in more than 70 % of non-small cell lung carcinomas and was associated with significantly worse overall patient survival. However, mechanisms regulating FUS1 protein expression and its inactivation in primary lung cancer cells are largely unknown. In this study, we investigated the role of the 5’- and 3’-untranslated regions (UTRs) of the FUS1 gene in the regulation of FUS1 protein expression and determined changes in FUS1 gene and protein expression in response to oncogenic stress in normal lung and cancer cell lines. We constructed a series of expression plasmids containing the FUS1 coding region with or without the intrinsic UTRs and transfected them into normal lung bronchial epithelial and tumor cells to assess their roles in regulation of FUS1 protein expression. We identified some critical structure elements in FUS1 UTRs that could significantly regulate FUS1 protein expression. We found that two small upstream open-reading frames (uORFs) encoding an 8-amino-acid peptide and another 6-amino-acid peptide, respectively, in the 5’UTR of FUS1 could markedly inhibit the translational initiation of FUS1 protein by interfering with the “scanning” of the ribosome initiation complexes. Several secondary RNA structural elements/motifs on the 3’UTR of FUS1 also showed a significant inhibitory effect on FUS1 protein expression. The 3’UTR-mediated regulatory effect on FUS1 proteins expression was differentially detected in normal lung epithelial and fibroblast cells compared to lung cancer cells. We found that FUS1 expression could be induced or activated in the presence of DNA damaging agents, such as UV irradiation, etoposide, and cisplatin. UV irradiation could induce FUS1 protein and mRNA expression independent of the status of FUS1 UTRs. The FUS1 expression activated by DNA damaging agents such as etoposide or cisplatin facilitated FUS1-induced apoptosis, prevented cells from dividing and accelerated cell death. Our results provide new insight into the molecular mechanisms in the regulation of FUS1 expression in lung cancer cells. (Supported by NIH SPORE P50CA70907 and RO1CA116322 grants) Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3083.
P2-02-05: Molecular Mechanism for Src Homology Phosphotyrosyl Phosphatase 2 Regulation of Cell Motility and Migration.
Authors: ZR Hartman, YM Agazie
Journal: Cancer Research (2011)

Abstract Background: The Src homology phosphotyrosyl phosphatase 2 (SHP2) acts as a transducer of mitogenic and cell survival signaling downstream of receptor tyrosine kinases, cytokine receptors and integrins. As such, SHP2 promotes cell growth, transformation, survival, and motility, among other processes. Recent studies suggest that SHP2 is overexpressed in breast cancer and is essential for the maintenance of the transformed phenotype both in the HER2−positive and the basal-type/triple-negative breast cancer (BTBC) cells. Materials and Methods: The expression of SHP2 in BTBC cells was suppressed constitutively by lenti virus-mediated transduction of specific shRNA. Impact of SHP2 inhibition on cell migration was determined by the monolayer wound healing assay. The SHP2 substrate-trapping mutant was used to identify FAK as an SHP2 substrate. Site-directed mutagenesis, binding studies and phosphatase assays were employed to further characterize FAK as a bona fide SHP2 substrate. Immunofluorescence microscopy was conducted to visualize the amounts of phosphorylated FAK and its location in the cell after adhesion. Results: We demonstrate that SHP2 is essential for the migration of BTBC cells as evidenced by loss of the enhanced migratory behavior upon its inhibition. Using the SHP2 substrate-trapping mutant, FAK was identified as a biological substrate of SHP2. Site-directed mutagenesis, binding studies and phosphatase assays confirmed that SHP2 indeed associates with and dephosphorylates FAK at the pY397 site, a site known to promote the focal adhesion activity of FAK. Immunofluorescence of breast cancer cells with SHP2 inhibition were found to have higher levels of FAK pY397 further confirming that FAK is an SHP2 substrate. The increase in pY397 level correlated with loss of cell polarity, enhanced focal adhesion formation, and acquisition of non-transformed morphology. Discussion: These results suggest that dephosphorylation of pY397 of FAK by SHP2 is the major mechanism by which it is able to modulate cancer cell migration. Together with previous findings, the current studies show that SHP2 is a suitable target for therapeutic intervention to combat breast cancer metastasis. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P2-02-05.
Lobbying bill could curtail public health campaigns, warns BMA
Authors: Z. Kmietowicz
Journal: BMJ (2013)

Doctors’ leaders have called on the UK government to pause and reconsider its proposed bill on lobbying. They say that the legislation could severely limit organisations such as the BMA that campaign on public health issues, including smoking, during an election year.

The warning was issued in a briefing paper by the BMA the day before the House of Lords was due to hold its second reading of the Transparency of Lobbying, Non-Party Campaigning and Trade Union Administration Bill on 22 October.1

The bill was introduced into the House of Commons in July this year with the aim of honouring the prime minister’s pre-election promise to end lobbying scandals. But many organisations have complained at …
RESECCIÓN ENDOSCÓPICA MUCOSA Y CIRUGÍA ENDOSCÓPICA TRANSANAL EN PÓLIPOS RECTALES GRANDES
Authors: MA Alvarez Gonzalez, L Hernandez, X Bessa Carreres, M Pascual Damieta, A Seoane Urgorri, L Carot Bastard, A García Rodriguez, L Márquez Pérez, M Andreu García, L Barranco Priego, F Bory Ros
Journal: Endoscopy (2013)

Introducción: Los adenomas rectales grandes actualmente se tratan mediante resección endoscópica mucosa (REM) o cirugía endoscópica transanal (CET). Aunque la REM puede presentar menor morbilidad, costes y molestias para el paciente, hay dudas de su eficacia. Métodos: Análisis retrospectivo de la serie de pólipos rectales>= 20 mm del programa de cribaje poblacional (PCP) tratados mediante REM (Periodo 2010 – 2011) y de la serie de casos consecutivos de pólipos rectales grandes tratados mediante CET (2010 – 2012). Resultados: 19 REM: tamaño medio 26 mm (IC 95% 22 – 31), 3 adenocarcinomas (T1) y 16 adenomas. Dos casos se ampliaron con CET por invasividad (T1) y 10 se revisaron con endoscopia, en 3 de 10 (33%) hubo adenoma residual y se realizó una segunda REM. Eficacia global de REM: 100% de los casos revisados. Complicaciones: 1 casos de dolor e ingreso un día. 16 CET: 9 como primer tratamiento, 3 como segundo tratamiento tras REM incompleta y 4 para ampliar radicalidad (T1). Como primer tratamiento: tamaño medio 29 mm (IC 95% 24 – 35), 6 adenocarcinomas (2 T2, 3 T1 y un Tis) y 3 adenomas. La CET fue el tratamiento definitivo excepto los dos T2. En 3 las 4 CET por ampliación de radicalidad (T1) no se demostró lesión residual. En uno de los 3 CET de rescate por REM incompleta no hubo lesión residual y en los otros fue el tratamiento definitivo. Complicaciones: 1 hemorragia precoz que requirió reintervención, 3 hemorragias tardías con resolución espontánea sin requerimiento transfusional y dos episodios de fiebre. Mediana de ingreso: 4 (IC 95%: 2 – 5) días. Conclusiones. La REM es una técnica eficaz y segura en pólipos rectales grandes, permite la resección de la lesión en un solo acto y evitar el ingreso. La CET es una técnica eficaz en adenomas no resecables endoscópicamente por dificultad técnica y sospecha de invasividad T1.
A Study of Australia-Antigen-Positive Blood Donors and Their Recipients, with Special Reference to Liver Histology
Authors: Vagn Reinicke, Esben Dybkjaer, Hemming Poulsen, Ole Banke, Kirsten Lylloff, Erik Nordenfelt
Journal: New England Journal of Medicine (1972)

Systematic screening of 13,300 consecutively registered Danish voluntary blood donors revealed 24 donors with persistent Au-antigenemia. None of these had any clinical signs of disease. Liver biopsies were performed on all the Au-antigenemic donors. In only one of the 24 biopsies was cirrhosis demonstrated. None of the remaining 23 biopsies, of which six showed a completely normal histologic picture, exhibited the changes seen in viral hepatitis. Before detection of the antigenemia 10 of the Au-antigenemic donors had given blood to 22 recipients. A retrospective study gave no evidence of past or present acute hepatitis in any of these recipients. No Au-antigen or Au-antibody was found in any of the surviving recipients. The apparent absence of post-transfusion hepatitis in the recipients may be related to the absence of acute and chronic hepatitis in the blood donors.
SP0202 Advanced Case Presentations: Interaction and Discussion
Authors: R. G. W. Lambert
Journal: Annals of the Rheumatic Diseases (2013)

<h3></h3> A series of cases will be presented that highlight and emphasize the important points of MRI interpretation of the manifestations of arthritis. This will include both frequent and unusual MRI findings encountered in common clinical conditions and typical, though often misunderstood, capabilities and limitations of MRI. Audience participation will be incorporated throughout this presentation. <h3>Disclosure of Interest</h3> R. Lambert Consultant for: AbbVie and Synarc