tbuckley/cabot-search huggingface dataset:
TCTAP A-132 Integration of Stress Myocardial Blood Flow, Coronary Flow Reserve, and Relative Flow Reserve Improves Diagnostic Performance of Myocardial Perfusion Imaging for Identification of Functionally Significant Coronary Artery Disease
Authors: Joo Myung Lee, Chee Hae Kim, Bon-Kwon Koo, Doyeon Hwang, Jonghanne Park, Ki-Hyun Jeon, Ji-In Bang, Minseok Suh, Jin Chul Paeng, Gi Jeong Cheon, Sang-Hoon Na, Seung-Jung Park, Hyo-Soo Kim
Journal: Journal of the American College of Cardiology (2016)
TCTAP C-215 “Pseudo Fogarty Thrombectomy” Using Sheathless OPTIMO (Temporary Occlusion Balloon Guiding Catheter) in Patient with Acute Limb Ischemia Due to Thrombotic Occlusion in Artificial Graft
Authors: Ryo Fukui, Hitoshi Anzai
Journal: Journal of the American College of Cardiology (2016)
Surface‐Mediated Stimuli Responsive Delivery of Organic Molecules from Porous Carriers to Adhered Cells
Authors: Bahar Ergün, Luisa De Cola, Hans‐Joachim Galla, Nermin Seda Kehr
Journal: Advanced Healthcare Materials (2016)

The alternating layer-by-layer deposition of oppositely charged polyelectrolytes on fluorescence-dye-(Hst)-loaded zeolites L ((Hst) Zeo-PSS/PLL) is described. The arrays and nanocomposite (NC) hydrogels of (Hst) Zeo-PSS/PLL are prepared. The subsequent cell experiments show the potential application of arrays and NC hydrogels of (Hst) Zeo-PSS/PLL as alternative 2D- and 3D-surfaces, respectively, for 2D- and 3D-surface-mediated controlled organic molecules delivery applications.
CD163-Macrophages Are Involved in Rhabdomyolysis-Induced Kidney Injury and May Be Detected by MRI with Targeted Gold-Coated Iron Oxide Nanoparticles
Authors: Alfonso Rubio-Navarro, Mónica Carril, Daniel Padro, Melanie Guerrero-Hue, Carlos Tarín, Rafael Samaniego, Pablo Cannata, Ainhoa Cano, Juan Manuel Amaro Villalobos, Ángel Manuel Sevillano, Claudia Yuste, Eduardo Gutiérrez, Manuel Praga, Jesús Egido, Juan Antonio Moreno
Journal: Theranostics (2016)

Macrophages play an important role in rhabdomyolysis-acute kidney injury (AKI), although the molecular mechanisms involved in macrophage differentiation are poorly understood. We analyzed the expression and regulation of CD163, a membrane receptor mainly expressed by anti-inflammatory M2 macrophages, in rhabdomyolysis-AKI and developed targeted probes for its specific detection in vivo by MRI. Intramuscular injection of glycerol in mice promoted an early inflammatory response, with elevated proportion of M1 macrophages, and partial differentiation towards a M2 phenotype in later stages, where increased CD163 expression was observed. Immunohistological studies confirmed the presence of CD163-macrophages in human rhabdomyolysis-AKI. In cultured macrophages, myoglobin upregulated CD163 expression via HO-1/IL-10 axis. Moreover, we developed gold-coated iron oxide nanoparticles vectorized with an anti-CD163 antibody that specifically targeted CD163 in kidneys from glycerol-injected mice, as determined by MRI studies, and confirmed by electron microscopy and immunological analysis. Our findings are the first to demonstrate that CD163 is present in both human and experimental rhabdomyolysis-induced AKI, suggesting an important role of this molecule in this pathological condition. Therefore, the use of probes targeting CD163-macrophages by MRI may provide important information about the cellular composition of renal lesion in rhabdomyolysis.
Conformationally Strained <i>trans-</i>Cyclooctene (sTCO) Enables the Rapid Construction of <sup>18</sup>F-PET Probes via Tetrazine Ligation
Authors: Mengzhe Wang, Dennis Svatunek, Katarina Rohlfing, Yu Liu, Hui Wang, Ben Giglio, Hong Yuan, Zhanghong Wu, Zibo Li, Joseph Fox
Journal: Theranostics (2016)

The bioorthogonal reaction between tetrazines and trans-cyclooctenes is a method for the rapid construction of F-18 probes for PET imaging.Described here is a second generation 18 F-labeling system based on a conformationally strained trans-cyclooctene (sTCO)-a dienophile that is approximately 2 orders of magnitude more reactive than conventional TCO dienophiles.Starting from a readily prepared tosylate precursor, an 18 F labeled sTCO derivative ( 18 F-sTCO) could be synthesized in 29.3 +/-5.1% isolated yield and with high specific activity.Tetrazine ligation was carried out with a cyclic RGD-conjugate of a diphenyl-s-tetrazine analogue (RGD-Tz) chosen from a diene class with an excellent combination of fast reactivity and stability both for the diene as well as the Diels-Alder adduct.For both the tetrazine and the sTCO, mini-PEG spacers were included to enhance solubility and improve the in vivo distribution profile of the resulting probe.Extremely fast reactivity (up to 2.86 x 10 5 M -1 s -1 at 25 °C in water) has been observed in kinetic studies in the reaction of sTCO with diphenyl-s-tetrazine derivatives.A kinetic study on sTCO diastereomers in 55:45 MeOH:water showed that the syn-diastereomer displayed slightly faster reactivity than the anti-diastereomer.An 18 F-sTCO conjugate with RGD-Tz demonstrated prominent and persistent tumor uptake in vivo with good tumor-to-background contrast.Unlike most radiolabeled RGD peptides, the tumor uptake of this PET agent increased from 5.3 +/-0.2%ID/g at 1 h post injection (p.i.), to 8.9 +/-0.5% ID/g at 4 h p.i., providing evidence for prolonged blood circulation.These findings suggest that tetrazine ligations employing 18 F-sTCO should serve as a powerful and general platform for the rapid construction of peptide or protein derived PET agents.
P1142 : Next generation sequencing analysis of cholestatic patients reveals high impact variation in common and rare candidate genes: Dissecting the genetic architecture of cholestasis
Authors: R. Liebe, A. Christmann, M. Krawczyk, P. Milkiewicz, F. Grünhage, C. Jüngst, F. Lammert
Journal: Journal of Hepatology (2015)
Interleukin-6 Induced “Acute” Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics
Authors: Ting Xue, Ping Liu, Yong Zhou, Kun Liu, Li Yang, Robert L. Moritz, Wei Yan, Lisa X. Xu
Journal: Theranostics (2016)

Cryo-thermal therapy has been emerged as a promising novel therapeutic strategy for advanced breast cancer, triggering higher incidence of tumor regression and enhanced remission of metastasis than routine treatments.To better understand its anti-tumor mechanism, we utilized a spontaneous metastatic mouse model and quantitative proteomics to compare N-glycoproteome changes in 94 serum samples with and without treatment.We quantified 231 highly confident N-glycosylated proteins using iTRAQ shotgun proteomics.Among them, 53 showed significantly discriminated regulatory patterns over the time course, in which the acute phase response emerged as the most enhanced pathway.The anti-tumor feature of the acute response was further investigated using parallel reaction monitoring target proteomics and flow cytometry on 23 of the 53 significant proteins.We found that cryo-thermal therapy reset the tumor chronic inflammation to an "acute" phenotype, with up-regulation of acute phase proteins including IL-6 as a key regulator.The IL-6 mediated "acute" phenotype transformed IL-4 and Treg-promoting ICOSL expression to Th1-promoting IFN-γ and IL-12 production, augmented complement system activation and CD86 + MHCII + dendritic cells maturation and enhanced the proliferation of Th1 memory cells.In addition, we found an increased production of tumor progression and metastatic inhibitory proteins under such "acute" environment, favoring the anti-metastatic effect.Moreover, cryo-thermal on tumors induced the strongest "acute" response compared to cryo/hyperthermia alone or cryo-thermal on healthy tissues, accompanying by the most pronounced anti-tumor immunological effect.In summary, we demonstrated that cryo-thermal therapy induced, IL-6 mediated "acute" microenvironment shifted the tumor chronic microenvironment from Th2 immunosuppressive and pro-tumorigenic to Th1 immunostimulatory and tumoricidal state.Moreover, the magnitude of "acute" and "danger" signals play a key role in determining the efficacy of anti-tumor activity.
Integration Of CT-Based Measurements Into PET Assessment Significantly Improves The Positive Predictive Value Of Surveillance FDG PET/CT In Patients With Diffuse Large B Cell Lymphoma
Authors: Noa Lavi, Gali Shapira, Ariel Zilberlicht, Noam Benyamini, Dan Farbstein, Eldad J Dann, Rachel Bar-Shalom, Irit Avivi
Journal: Blood (2013)
Surface Properties of Normal, Transformed, and Temperature-Sensitive Rat Liver Epithelial Cells&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: N I Goldstein, P B Fisher
Journal: JNCI Journal of the National Cancer Institute (1981)

Cell surface proteins, glycoproteins, and glycopeptides from normal, transformed, and mutant temperature-sensitive rat liver epithelial cells were radiolabeled and compared by Sephadex G-50 chromatography and polyacrylamide gel electrophoresis. Some changes were observed in the transformed cell lines including an increase in high-molecular-weight glycopeptides and a decrease in the amount of fibronectin (large external transformation-sensitive) protein. The mutant TS223 cells, which were temperature-sensitive for maintenance of the transformed phenotype when grown at the nonpermissive temperature (41 degrees C), had characteristics intermediate between those of the normal and transformed phenotypes. These results corresponded to those of previous studies indicating that TS223 was somewhat "leaky" at 41 degrees C; i.e., although more "normal" than at 37 degrees C, it was not completely normal. Alterations in surface macromolecules in transformed epithelial cells may, therefore, represent a useful marker for transformation because morphologic changes are usually not readily apparent in transformed epithelial cells.
Ultrasound-Image-Based Cardiovascular Tissue Motion Estimation
Authors: Spyretta Golemati, Aimilia Gastounioti, Konstantina S. Nikita
Journal: IEEE Reviews in Biomedical Engineering (2016)

The estimation of cardiovascular tissue motion from ultrasound images is a task of considerable importance but has remained difficult in clinical practice, mainly due to the limitations of ultrasound imaging and the complexity of tissue motion. This paper presents a survey of methodologies, along with physiologically relevant findings, regarding the estimation of motion of the myocardium and of central and peripheral arteries. Speckle tracking and modeling, and registration are the dominant methods used to calculate tissue displacements from sequences of images. Kinematic and mechanical indices are extracted from these displacements, which can provide valuable functional information about the cardiovascular system in normal and diseased conditions. An important application of motion-based strain indices involves the estimation of elastograms of the cardiovascular tissue. Motion analysis methods can be used to estimate a number of regional mechanical phenomena representing functional tissue properties, which are more sensitive to early changes due to ageing or disease. The importance of these methods lies in their potential to quantify in vivo tissue properties and to identify novel noninvasive personalized disease markers, toward early detection and optimal management of disease, along with increased patient safety. Their clinical usefulness remains to be demonstrated in large trials.
TCTAP C-251 The Staged Minimally-Invasive Treatment in Patient with Severe, Symptomatic Aortic Stenosis and Descending Aortic Aneurysm
Authors: Pawel E. Buszman, Wojciech Fil, Justyna Jankowska Sanetra, Marcin Debinski, Wojciech Domaradzki, Marek Cisowski
Journal: Journal of the American College of Cardiology (2016)
Use of homologous recombination deficiency (HRD) score to enrich for niraparib sensitive high grade ovarian tumors.
Authors: Keith Matthew Wilcoxen, Marc Becker, Christopher Neff, Victor Abkevich, Joshua Timothy Jones, Xiaonan Hou, Yan Wang, Anne-Renee Hartman, Mariam M. AlHilli, Alexander Gutin, Shefali Agarwal, Kirsten Timms, Paul Haluska
Journal: Journal of Clinical Oncology (2015)

5532 Background: The therapeutic potential of PARP inhibitors is predicted to extend beyond patients with germline BRCA mutations (BRCAmut) to those with homologous recombination deficient (HRD) cancers. An HRD test is being applied to patient samples to identify BRCA mutated tumors, provide a categorical determination of tumor HRD, and determine an enrichment strategy for patients with tumors that are sensitive to niraparib treatment. Methods: Archival FFPE tumor tissue obtained from ovarian cancer patients enrolled in an ongoing Phase III clinical study (ENGOT-OV16/NOVA, NCT 01847274) and a living tumor bank was subjected to HRD testing. The final HRD score is a numeric output ranging from 0-100. A predefined cut-off score of 42 was utilized to determine HRD status of each tumor and classify it as HR deficient (HRD score ≥ 42) or non-deficient (HRD score < 42). Classification of BRCA mutations from tissue was based on analysis criteria consistent with the laboratory's approved diagnostic BRCA testing. Niraparib efficacy was evaluated utilizing a living tumor bank of orthotopic patient-derived ovarian xenografts. Intraperitoneal tumorgrafts were monitored for tumor growth with twice-weekly transabdominal ultrasound imaging. Results: One hundred and six high grade serous ovarian tumors from a living tumor bank were evaluated for HRD, BRCA1/2 mutations, and BRCA1 promoter hypermethylation. Twenty six tumors (25%) were BRCA deficient (a deleterious BRCA1/2 mutation or BRCA1 hypermethylation with a concomitant loss of heterozygosity). All BRCA1/2 deficient tumors had an HRD score of 42 or greater, with the exception of one tumor. The in-vivo response to niraparib monotherapy has been evaluated in 20 unique tumor models across a range of HRD scores. All responding tumors had an HRD score of 42 or above, irrespective of BRCA deficiency. Conclusions: HRD testing of ovarian cancer was predictive of BRCA deficiency. Response to niraparibwas only observed in HRD positive (HRD+) tumors. Our data are supportive of the use of HRD testing to enrich for niraparib responders in high grade ovarian cancer.
TCTAP C-023 Optical Coherence Tomography Control of the Results of Mesh-covered MGuard Stenting in Posterior ST-elevation Myocardial Infarction: Immediate and Long-term Results
Authors: Dzhamil Arif Asadov, Ilya Alexandrovich Kovalchuk, Dmitry Kurtasov, Victoria Victorovna Fomenko
Journal: Journal of the American College of Cardiology (2016)
Sequential vs alternating administration of VMP and Rd in elderly patients with newly diagnosed MM
Authors: María-Victoria Mateos, Joaquín Martínez-López, Miguel-Teodoro Hernández, Enrique-M. Ocio, Laura Rosiñol, Rafael Martínez, Ana-Isabel Teruel, Norma C. Gutiérrez, María-Luisa Martín Ramos, Albert Oriol, Joan Bargay, Enrique Bengoechea, Yolanda González, Jaime Pérez de Oteyza, Mercedes Gironella, Cristina Encinas, Jesús Martín, Carmen Cabrera, Bruno Paiva, María-Teresa Cedena, Noemí Puig, Joan Bladé, Juan-José Lahuerta, Jesús San-Miguel
Journal: Blood (2015)
IL-1 is a critical regulator of group 2 innate lymphoid cell function and plasticity
Authors: Yoichiro Ohne, Jonathan S Silver, LuAnn Thompson-Snipes, Magalie A Collet, Jean Philippe Blanck, Brandi L Cantarel, Alan M Copenhaver, Alison A Humbles, Yong-Jun Liu
Journal: Nature Immunology (2016)
Peroral video cholangioscopic findings in a biliary intraductal mucinous cystic neoplasm
Authors: Hiroshi Kawakami, Yoshimasa Kubota, Kimitoshi Kubo, Daisuke Sato, Tomoko Mitsuhashi, Satoshi Hirano, Naoya Sakamoto
Journal: Endoscopy (2016)

Mucinous cystic neoplasm (MCN) of the liver is a biliary counterpart of pancreatic MCN, usually occurring in the liver, but occasionally extending to the extrahepatic bile duct [1] [2]. We present a case of MCN with extrahepatic bile duct extension diagnosed by peroral video cholangioscopy (POVCS).
Abstract 2574: HIPEC for treatment of peritoneal carcinomatosis: 40°C is the critical threshold temperature for potentiating chemotherapy efficacy in vitro and in vivo
Authors: Lea Schaaf, Marina Münch, Wolfgang Steurer, Walter E. Aulitzky, Thomas E. Mürdter, Heiko van der kuip, Christoph Ulmer
Journal: Cancer Research (2015)

Abstract Hyperthermic intraperitoneal chemotherapy (HIPEC) combines circulated chemotherapy with enhanced temperature in the abdominal cavity of peritoneal carcinosis (PC) patients. Unfortunately, a standardized HIPEC protocol is missing hindering systematic comparisons with regard to minimal effective temperatures. The purpose of the present study was to systematically analyse the temperature needed for potentiation of chemotherapy effects in vitro. We then asked if this temperature could be sustained in patients during HIPEC and evaluated possible effects on survival. We therefore established a cell line-based model to mimic HIPEC conditions used in clinic. We evaluated intracellular drug concentrations and long-term survival using different temperatures ranging from 38 to 42°C combined with Cisplatin and Doxorubicin. In parallel we evaluated the temperature reached in the clinical setting by measuring inflow and outflow, as well as in the peritoneal cavity in 36 PC patients. Finally we determined the influence of different temperatures on survival. In vitro, we found temperature-dependent effects on intracellular drug concentration and long term survival only at temperatures above 40°C. In patients, during HIPEC, constant temperatures were reached after 10 minutes in the peritoneal cavity. A temperature above 40°C for at least 45 minutes was achieved in 67% or 44% of patients with HIPEC in open or closed abdomen, respectively. Importantly, we observed a significantly enhanced overall survival as well as a trend towards longer recurrent free survival in those patients with HIPEC temperatures above 40°C. In conclusion, hyperthermia significantly potentiated the chemotherapy effects only at temperatures above 40°C in vitro. Importantly, this temperature threshold was also critical for overall and recurrent-free survival of PC patients. Citation Format: Lea Schaaf, Marina Münch, Wolfgang Steurer, Walter E. Aulitzky, Thomas E. Mürdter, Heiko van der kuip, Christoph Ulmer. HIPEC for treatment of peritoneal carcinomatosis: 40°C is the critical threshold temperature for potentiating chemotherapy efficacy in vitro and in vivo. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2574. doi:10.1158/1538-7445.AM2015-2574
Case 38-2004
Authors: Paul G. Richardson, Ara Kassarjian, Wen Jing
Journal: New England Journal of Medicine (2004)

A 40-year-old man noted a lump on the top of his head. Imaging studies showed a dural-based tumor invading bone and soft tissue. The lesion was excised and a diagnosis of plasmacytoma was made. Additional staging studies disclosed multiple lytic bone lesions, with no paraprotein and a negative bone marrow biopsy and aspirate. The discussants review recent advances in the management of multiple myeloma.
Phenotypic research on senile osteoporosis caused by SIRT6 deficiency
Authors: De-Mao Zhang, Di-Xin Cui, Ruo-Shi Xu, Ya-Chuan Zhou, Li-Wei Zheng, Peng Liu, Xue-Dong Zhou
Journal: International Journal of Oral Science (2016)

Osteoporosis is a serious public bone metabolic disease. However, the mechanisms underlying bone loss combined with ageing, which is known as senile osteoporosis, remains unknown. Here we show the detailed phenotype of this disease caused by SIRT6 knock out (KO) in mice. To the best of our knowledge, this is the first study to reveal that SIRT6 is expressed in both bone marrow stroma cells and bone-related cells in both mouse and human models, which suggests that SIRT6 is an important regulator in bone metabolism. SIRT6-KO mice exhibit a significant decrease in body weight and remarkable dwarfism. The skeleton of the SIRT6-KO mouse is deficient in cartilage and mineralized bone tissue. Moreover, the osteocalcin concentration in blood is lower, which suggests that bone mass is markedly lost. Besides, the tartrate-resistant acid phosphatase 5b (TRAP5b) concentration is much higher, which suggests that bone resorption is overactive. Both trabecular and cortical bones exhibit severe osteopenia, and the bone mineral density is decreased. Moreover, double-labelling analysis shows that bone formation is much slower. To determine whether SIRT6 directly regulates bone metabolism, we cultured primary bone marrow stromal cells for osteogenesis and osteoclastogenesis separately to avoid indirect interference in vivo responses such as inflammation. Taken together, these results show that SIRT6 can directly regulate osteoblast proliferation and differentiation, resulting in attenuation in mineralization. Furthermore, SIRT6 can directly regulate osteoclast differentiation and results in a higher number of small osteoclasts, which may be related to overactive bone resorption.
End-of-life care for elderly patients with advanced lung cancer in the United States and Ontario.
Authors: K. Yabroff, J. Warren, L. C. Barbera, K. Bremner, J. Hoch, M. Barrett, J. Luo, M. Krahn
Journal: Journal of Clinical Oncology (2010)

6021 Background: Although the health care systems in the U.S. and Canada differ, both have government-sponsored health insurance for the elderly. Approaches to end of life care also vary between the U.S., which offers Medicare's comprehensive hospice program, and Canada, which has a fragmented system of palliative care. Little research has compared end of life care for elderly in the two countries. Purpose: To compare the types and intensity of end of life care in elderly non-small cell lung cancer (NSCLC) patients in the U.S. and the province of Ontario, Canada. Methods: The linked SEER-Medicare data were used to identify care for 12,198 NSCLC patients with advanced disease at diagnosis who died of cancer between 1999 and 2003 in the U.S. Comparable NSCLC patients (n = 8,100) from the Ontario Cancer Registry were linked with their health claims. Outcomes evaluated were use of chemotherapy, ER visits, inpatient hospitalizations, supportive care, and deaths in hospital. Rates of service use were estimated by person-months and adjusted for age, income, sex, and urbanicity. Results: Rates of chemotherapy use were higher in the U.S. than in Ontario, and declined during the last six months of life. Rates of hospice and palliative care use increased dramatically during the last six months, and in the last 30 days, more than half of patients received hospice care in the U.S. or palliative care in Ontario. During the last 30 days, patients in Ontario were more likely than in the U.S. to have an ER visit (50.7% vs. 39.8%), be hospitalized (54.4% vs. 45.9%), and die in hospital (51.9% vs. 20.3%). Conclusions: NSCLC patients received extensive acute and supportive care services during the last six months of life. Differences in end of life care between the U.S. and Ontario reflect the availability of comprehensive approaches to end of life care as well as attitudes of physicians and patients. Determining the optimal model of end of life care requires consideration of factors such as patient and family quality of life, as well as health system and patient-borne costs. No significant financial relationships to disclose.
Endoscopic mucosal resection of duodenal carcinoid
Authors: Nikhil Kumta, Amit Desai, Rushabh Doshi, Michel Kahaleh, Reem Sharaiha
Journal: Endoscopy (2016)

A 45-year-old man with a history of renal cell carcinoma and immunosuppression presented to our institution with melena. Upper gastrointestinal endoscopy demonstrated two nodules in the duodenal bulb. Biopsies revealed a circumscribed neoplasm in the duodenal submucosa comprising nests of epithelial cells with uniform round nuclei and abundant finely granular amphophilic cytoplasm. Chromogranin immunostaining confirmed the diagnosis of a well-differentiated neuroendocrine tumor consistent with carcinoid ([Fig. 1]).