tbuckley/cabot-search huggingface dataset:
SAT0318 Antisynthetase Syndrome: Clinical and Serological Characteristics at Disease Onset: Table 1.
Authors: J. Martínez-Barrio, M. Hinojosa, J. Lopez-Longo, J.G. Ovalles-Bonilla, N. Bello, C. Mata Martínez, B. Serrano, J.C. Nieto Gonzalez, L. Martínez Estupiñan, C. Gonzalez, M. Montoro, L. Valor, I. Monteagudo, E. Naredo, L. Carreño
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Antisynthetase syndrome (ASS) is a rare condition characterized by the presence of one or more of the following clinical features: arthritis, Raynaud9s phenomenon, “mechanic9s hands”, interstitial lung disease and polymyositis, in patients with antisynthetase antibodies which target aminoacyl-tRNA synthetases enzymes, being Anti-Jo1 the most common one. <h3>Objectives</h3> To investigate the clinical features of ASS at disease onset and their influence on the disease clinical course. <h3>Methods</h3> 32 consecutive patients diagnose with ASS, were prospectively enrolled between 1988 and 2013. Inclusion criteria consisted of having a positive test for antisynthetase antibodies, regardless of fulfilling classification criteria for other rheumatic diseases. Serological, clinical and demographic features were registered both at clinical onset and during the course of the disease. Anti-synthetase antibodies were determined by LIA or ELISA. <h3>Results</h3> From 32 patients, 21 (65.6%) were females and 11 (34.4%) were males. Mean age at clinical onset was 47.4 years (SD 18.8) and the mean time of disease course was 11.1 years (SD 10.2). The most common feature at disease onset was arthritis in 50% of the patients (8 of them presenting arthritis&gt;6 weeks), followed by constitutional syndrome (31.3%) and muscular symptoms (25%). Table 1 shows the different clinical features and frequency of occurrence at onset. During the course of disease 28 patients (87.5%) developed musculoskeletal manifestations, 26 (81.3%) arthritis, 21 (65.6%) cutaneous manifestations, 20 (62.5%) interstitial lung disease and 13 (40.6%) Raynaud9s phenomenon. Associations between the presence of Anti-Ro52 and arthritis at disease onset (p=0.009), interstitial lung disease (p=0.002) and low complement levels (p≤0.001) were found. <h3>Conclusions</h3> In our study, clinical features at onset were very heterogeneous. Patients showed neither all clinical features at the same time nor during the disease course. In our cohort, Anti-Ro52 antibody was associated to both the presence of arthritis at onset and the development of interstitial lung disease, which could be relevant for differential diagnosis and prognosis. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.3113
THU0359 Half of High School Students Playing Sports Experience Injuries, Even More with Contact Sports: Time for New Regulations?
Authors: D.C. Ince, C.J. Swearingen, Y. Yazici
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Many students participate in sports during high school, with many of them taking part in multiple sports depending on the season. Injuries sustained during these activities may impact future participation and overall health of the student. <h3>Objectives</h3> To determine the prevalence, rate and possible risk factors for high school sports injuries <h3>Methods</h3> A sports injury survey was administered to high school students9 self-reporting sports activity during the past year. Sport played, level of play (varsity, junior varsity or club level), occurrence of injury and duration of injury were recorded. 15 sports were queried in the survey; sports were classified into contact sports vs non-contact sports. Statistical analysis of the categorical data was completed using Chi-square test of independence and logistic regression. <h3>Results</h3> 214 high school students reporting some level of sports activity over the previous year completed the injury survey; 109 (51%) were male and average 16.2 (±1.1) years of age. 105 (49%) participants reported suffering an injury during the previous year, with 64 (59%) of the injuries lasting less than three weeks in duration. Of these 105 injuries, 68 (65%) occurred while playing a contact sport (defined as soccer, football, basketball, wrestling, lacrosse, ice and field hockey). For those playing a contact sport, unadjusted odds of receiving an injury were 3300% increased over those playing a non-contact sport (OR=34.0, 95% CI:(8.3, 138.7), P&lt;0.001); logistic regression model adjusting for age and gender also estimated a significant effect. In a logistic regression model, an association between injury lasting three weeks or longer and level of sport played, contact sport, gender and age failed to be statistically established. <h3>Conclusions</h3> ∼50% of high school students participating in sports, and 65% of those paying contact had an injury. Most injuries lasted less than 3 weeks. Long-term impact of these injuries needs further study. These high rates of injury to high school age children may require possible more oversight and regulation of these activities. <h3>Disclosure of Interest</h3> : None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5543
Dual-focus narrow band imaging for the detection of intestinal metaplasia and atrophic gastritis
Authors: Gian Tontini, Annette Lindner, Michael Vieth, Maurizio Vecchi, Markus Neurath, Helmut Neumann
Journal: Endoscopy (2014)

Gastric cancer is one of the most common neoplastic diseases in developed Western countries, and is characterized by a poor prognosis when diagnosed at a late stage [1]. Atrophic gastritis and gastric intestinal metaplasia (GIM) are well-known risk factors for the development of gastric cancer. For this reason, endoscopy with multiple biopsies is recommended in patients with atrophic gastritis or GIM, to exclude preneoplastic or neoplastic tissue [1]. Magnifying endoscopy with narrow band imaging (NBI) has been shown to be reliable for in vivo diagnosis of atrophic gastritis and GIM [1] [2] [3] [4]. However, while magnifying endoscopy is routinely used by Asian endoscopists, it is not established in most European and Northern American endoscopy centers. Recently, a novel endoscope with NBI and dual-focus capability has been introduced to improve the quality of endoscopic imaging [5]. Dual focus allows the user to select between two focus settings by pushing a button on the scope. This results in an optimized close view of the tissue up to an 80-fold optical magnification. To the best of our knowledge, no reports exist so far on the use of this new technique for the detection and characterization of gastric lesions. We report a case in which dual-focus narrow band imaging was used to diagnose atrophic gastritis with intestinal metaplasia and obtain targeted biopsies in the same session.
Abstract P1-05-14: Multiscale computational modeling of breast cancer invasion: Towards a predictive patient-based tool
Authors: D Trucu, A Thompson, MAJ Chaplain
Journal: Cancer Research (2012)

Abstract Background: Understanding the mechanisms of the growth and spread of cancer cells in the human breast and providing effective treatment continues to remain a significant challenge. Cancer cell invasion of breast tissue plays a pivotal role in the metastatic cascade through complex, coupled dynamics of the constituent processes occurring over a range of spatial and temporal scales, ranging from genetic events through intracellular signaling pathways through cell-matrix interactions to tissue scale changes. Method: While most previous computational modeling has been carried out at the level of a single spatial or temporal scale, the work presented here has developed a novel multiscale modeling and computational simulation framework. Formulated in both two and three spatial dimensions, this approach is based on a new type of multiscale moving boundary computational model that explores the crucial role that matrix degrading enzymes, secreted by individual cancer cells (micro-scale) along the invasive edge of the tumour, play in the overall dynamics of the tissue-scale invasion (macro-scale). Results: The computational simulation results we have obtained reveal a rich variety of cancer invasion patterns that emerge in the vicinity of a primary breast cancer. In the presence of heterogeneous tissue, our computational simulation results show well-defined “lobular” and “fingering” invasion patterns, consistent with the spread of a breast carcinoma, and which remain robust with respect to any changes made in the model parameters. These patterns are found to be compatible with respect to both the dynamic heterogeneity of cancer cell macro-scale distributions and to the evolution of the heterogeneity of the surrounding breast tissue. Conclusions: By exploring the dynamics of the molecular processes of matrix degrading enzymes and of tissue-scale spatio-temporal cancer tissue evolution, this novel multiscale computational modeling framework faithfully replicates the cancer invasion process. Development of this computational tool should allow pre-operative breast cancer patient data to be used to predict the extent of breast cancer invasion and hence the extent of surgical resection. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P1-05-14.
SIGNIFICADO DEL LÍQUIDO LIBRE PERIGÁSTRICO IDENTIFICADO POR ECOENDOSCOPIA EN PACIENTES CON CÁNCER GÁSTRICO
Authors: A Repiso, C Arribas, C Navarro, R Gómez, JM Carrobles
Journal: Endoscopy (2011)

Objetivos: Determinar el diagnóstico final de los pacientes con cáncer gástrico y líquido libre perigástrico identificado por ecoendoscopia y establecer los factores relacionados con la presencia de carcinomatosis peritoneal en estos pacientes.
F-18 FDG PET/CT of a Uterine Smooth-Muscle Tumor of Unknown Malignant Potential
Authors: Il Ki Hong, Deog Yoon Kim
Journal: Clinical Nuclear Medicine (2011)

We report a patient who was found to have a smooth-muscle tumor of unknown malignant potential on F-18 FDG PET/CT. A 49-year-old woman was admitted because of abdominal distension of 6-month duration. CT showed a 10 × 17 × 19 cm mass at the posterior side of the uterus displacing the uterus to the right anterior side, which was thought to be a subserosal myoma or a leiomyosarcoma originated from the uterus. F-18 FDG PET/CT showed a large mass demonstrating heterogeneous FDG uptake. Total abdominal hysterectomy, bilateral salpingo-oophorectomy, and pelvic lymphadenectomy were performed. The mass was diagnosed as a smooth-muscle tumor of unknown malignant potential.
SP0175 Methodological issues with observational data: Confounding by indication and missing data
Authors: M. Lunt
Journal: Annals of the Rheumatic Diseases (2013)

Missing data is very common in observational studies, and the data may be missing for a number of reasons. Missing data will cause estimates to be less precise than they would otherwise have been. They may also result in biased estimates, depending on the mechanism causing them to be missing and the methods used to analyse the observed data. A second common problem in observational studies of the effect of a particular treatment or exposure is confounding by indication: the decision to treat or not treat may depend on patient characteristics which affect the outcome. Therefore, the difference in outcome between treated and untreated subjects is caused partly by the treatment and partly by this difference in patient characteristics, and hence is a biased measure of the effect of treatment. The true effect of treatment is the difference between the outcome if the patient receives treatment and the outcome if they do not receive treatment. Since only one of these outcomes can ever be observed, estimating the effect of treatment can also thought of as a missing data problem. I will present methods which are commonly used to overcome the problems of missing data and confounding by indication, concentrating on the conditions under which they enable the effect of treatment to be estimated without bias.I will also show the parallels between methods that give reliable inference when there is missing data, and methods that give reliable inference when there is confounding by indication. <h3>Disclosure of Interest</h3> None Declared
CELL INTRINSIC SEXUAL DIMORPHISM IN THE RB AND P21 PATHWAYS UNDERLIES MALE PREDOMINANCE IN GBM
Authors: J. B. Rubin, T. Sun, N. M. Warrington, J. Luo, M. Brooks, S. Dahiya, R. Sengupta
Journal: Neuro-Oncology (2014)

BACKGROUND: Male prevalence across histological subtypes of brain tumors is well known but unexplained. The effects of sex on human disease frequently result from acute actions of circulating sex hormones on cellular physiology and immune function. However, differences in brain tumor rates between males and females are comparable in prepubertal children, and pre and post-menopausal adults, suggesting that alternate mechanisms may underlie this disparity. Here we show that sex-specific differences exist in thresholds for malignant transformation within the astrocyte lineage and that this can contribute to male predominance in the most common brain tumor, glioblastoma (GBM). METHODS: Analysis of three publically accessible databases was utilized to determine whether sex differences are equally present in each of the four molecular subtypes of GBM. Stepwise transformation was modeled for Mesenchymal GBM in separate male and female murine astrocyte cultures by sequential inactivation of neurofibromin (NF1) and p53. Thresholds for transformation were measured in soft agar assays and with in vivo tumorigenesis assays. The molecular basis for differences in thresholds for transformation between male and female astrocytes was investigated in evaluations of stem cell induction, Rb inactivation and p21 activation. RESULTS: Significant male predominance was consistently observed in Proneural and Mesenchymal subtypes of GBM. We modeled Mesenchymal subtype GBM with combined loss of Nf1 and p53 function through cre-mediated knock-out of Nf1 (Nf1-/-) and expression of a dominant negative p53 construct (DNp53). These changes induced a subpopulation of cells with clonogenic, stem cell function in male but not female astrocytes. Male Nf1-/-,DNp53 astrocytes exhibited significantly greater growth compared to female Nf1-/-,DNp53 astrocytes. In addition, male but not female Nf1-/-,DNp53 astrocytes were transformed with either EGF treatment or in vivo implantation. Male Nf1-/-,DNp53 astrocytes exhibited greater in vivo tumorigencitiy regardless of the sex of the recipient mouse. Induced intracranial tumors were diagnosed as grade 4 astrocytomas based on standard histological features. Enhanced stem cell function and tumorigenicity in male Nf1-/-,DNp53 astrocytes was associated with greater inactivation of Rb and less p21 activation compared to female Nf1-/-,DNp53 astrocytes. CONCLUSIONS: Sex differences in cancer, including GBM can involve cell intrinsic sexual dimorphism in tumor suppressor pathways resulting in different thresholds for transformation in males and females. These results suggest that tumor suppressor and oncogene mechanisms should be evaluated in a sex-specific fashion and that screening and treatment of GBM and other cancers may need to be sex-specific. SECONDARY CATEGORY: n/a.
Abstract 690: Selection of resistance by anticancer drugs is not responsible for alterations in membrane micro-fluidity: A study by Fluorescence Correlation Micro-Spectroscopy
Authors: Pascale Winckler, Aurélie Cailler, Rodolphe Jaffiol, Pascal Royer, Pierre Jeannesson, Hamid Morjani
Journal: Cancer Research (2011)

Abstract Expression of P-glycoprotein (P-gp), the multidrug resistance (MDR) 1 gene product, can lead to MDR in tumors. Previous studies on multidrug-resistant cells have suggested changes in membrane fluidity associated with P-gp expression in drug-selected resistant cells. However, Aleman and co-workers have shown that, in MDR1 transfected cells, expression of P-gp has little effect on membrane fluidity and the changes in this parameter observed in drug-selected cells must reflect other host adaptations to drug selection. In this study and the other reported on drug-selected resistant cells, membrane fluidity has been measured using fluorescent membrane probes and fluorescence anisotropy or electron spin resonance on cell population samples (Aleman et al. Cancer Res. 2003 63:3084-91). Here, we propose to explore the plasma membrane microfluidity by using the fluorescent membrane probe DiA and fluorescence correlation micro-spectroscopy (FCM-S) on a single living cell and nanometer scale. By using the fluorescent membrane probe DiA and FCM-S to explore the plasma membrane micro-fluidity, we have previously shown a decrease in plasma membrane micro-fluidity in LR73R cells (the Chinese hamster ovarian LR73 cells transfected with MDR1 cDNA; Boutin et al., J Biomed Opt. 2009 14: 034030). Moreover, a strategy using flow cytometry and calcein-AM functional fluorescence-activated cell sorting permitted us to select two cell populations from LR73R cells expressing distinct levels of P-gp function. In this case, we were able to observe distinct level of membrane micro-fluidity in the two populations. Cells showing high level of calcein-AM uptake presented a higher membrane micro-fluidity. In contrast, the FCM-S yielded opposite data when we compared the membrane micro-fluidity of several parental cell lines (MCF7, KB3.1, LR73, ME-SSA) and their resistant counterparts which have been selected in the presence of anticancer drugs. Our data are not in agreement with previous published data and demonstrates clearly that alterations that can affect drug-selected cells do not induce any changes in membrane fluidity. Moreover, micro-analysis of cell membrane could allow to more precise measurements of membrane fluidity. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 690. doi:10.1158/1538-7445.AM2011-690
AB1163-HPR Effect of Stabilizing Exercises versus Tens in Fatigue of the Lumbar Multifidus Muscle and the Ability to Activate the Transversus Abdominis: A Preliminary Study
Authors: A.P. Marques, L.A.V. Ramos, F.J.R. França, B. Callegari, T.N. Burke, M.O. Magalhães, A.P.M.C. Carvalho e Silva, G.P.L. Almeida
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Chronic low back pain (cLBP) is defined as back pain lasting more than 12 weeks in the lumbar and sacral levels of the spine. The intervertebral disc herniation into the vertebral canal may cause pain and affects the transversus abdominis (TrA) and Lumbar Multifidus (ML) muscles that are preferentially attacked in episodes of nonspecific low back pain. <h3>Objectives</h3> Compare the effect of stabilizing exercises versus TENS in fatigue of the lumbar multifidus muscle (LM) and the ability to activate the transversus abdominis (TrA) in patients with lumbar disc herniation (LDH). <h3>Methods</h3> This study involved 29 patients (age range 25–58 years) randomised into two groups: Stabilisation group (SG: n=15); which received of stabilization exercises (transversus abdominis and lumbar multifidus muscles) and TENS group (TG: n=14), which received electrotherapy. Groups underwent 16 sessions, for 60 minutes, twice a week and they were evaluated before and after eight weeks. The following instruments were used: visual analogue pain scale and McGill Pain Questionnaire for pain, Oswestry Disability Index for functional disability, electromyography (EMG) to evaluate the LM and pressure biofeedback unit (PBU) for ability to contract the TrA. Analyses within and between groups were performed after treatment. One-way analysis of variance was used for intergroup and intragroup comparisons. <h3>Results</h3> The stabilisation group was superior to TENS in terms of improvements in fatigue of ML, pain, functional disability and ability to contract the TrA. There were no significant differences in TENS group in terms of disability or ability to contract the TrA muscle. <h3>Conclusions</h3> The stabilisation group was superior to TENS in improvements fatigue of ML, pain, functional disability and ability to contract the TrA. <h3>References</h3> Dedering A, Ringdahl KH, Ne`meth G. Back extensor muscle fatigue in patients with lumbar disc herniation, pre-operative and post-operative analysis of electromyography, endurance time and subjective factors, Eur Spine J, 2006, 15: 559–569. Cairms MC, Harrison K, Wright C. Pressure biofeedback: a useful tool in the quantification of abdominal muscular dysfunction? Physiother, 2000, 86(3): 127-38. <h3>Acknowledgements</h3> CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2927
Abstract 2496: Inhibition of IL-6 signaling induces radiosensitivity in head and neck squamous cell carcinoma (HNSCC)
Authors: Heath D. Skinner, Ying-Wen Su, Thomas J. Ow, Jeffery Myers
Journal: Cancer Research (2011)

Abstract Squamous cell carcinoma of the head and neck (HNSCC) is the sixth most common cancer worldwide. One of the primary modalities used to treat HNSCC is external radiotherapy, however many tumors are resistant to this treatment. Several studies have noted increased expression of IL-6 and IL-6 receptor in a variety of tumors. Furthermore, inhibition of IL-6 using a monoclonal antibody, tocilizumab, as a monotherapy has been shown to decrease tumor growth of HNSCC in pre-clinical models. However the effect of IL-6 signaling on radiosensitivity has not been well characterized. In this study we have found that baseline radioresistance, determined using a standard clonogenic assay, was significantly higher in HNSCC cell lines expressing high levels of IL-6 receptor (IL-6R) compared to those expressing low levels or IL6R as measured by RT-PCR (Surviving fraction at 2Gy (SF2) 65%% vs. 45%, p=0.028). In HN 31 and PCI 15B cell lines, which express high levels of IL-6 receptor, the addition of tocilizumab potentiated radiosensitivity, with a decrease in SF2 of 15% (p&amp;lt;0.05) in both cell lines. This finding was not observed in normal human fibroblasts. Also, the addition of tocilizumab inhibited radiation-induced survival signaling in a dose dependent fashion. Finally, we used immunohistochemistry to show that clinical samples of HNSCC from a variety of sites express IL-6 receptor, compared to little to no staining in surrounding normal tissue. Thus, the addition of tocilizumab to radiotherapy has the potential to enhance the radiosensitivity of HNSCC while minimizing the effect on normal tissue. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2496. doi:10.1158/1538-7445.AM2011-2496