tbuckley/cabot-search huggingface dataset:
AB0774 The Effect of 1 Year Oral Ibandronate on Bone Mineral Density and HR QoL – An Outpatient Study in A Speciality Clinic in Albania
Authors: B. Hebibasi
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Biphosphonate use is expected to increase Bone Mineral Density (BMD) and reduce the risk of vertebral and non-vertebral fractures. This may also lead to an improvement of Health Related Quality of Life (HR QoL) in the treated patients. <h3>Objectives</h3> To confirm the efficacy and safety of 1 year oral Ibandronate, 150 mg monthly. To analyze its effect on patients9 quality of life. <h3>Methods</h3> 140 postmenopausal patients (average age 65.2 yrs, avg age of menopause 46.8 yrs, avg years from menopause 18.3yrs) diagnosed with osteoporosis (T-score &lt;2.5 SD) during 2014 – 2015, were given 150mg of oral Ibandronic Acid monthly and supplements of Calcium and Vitamin D daily. BMD was measured with DXA HOLOGIC at baseline and after 1 year of treatment. HR QoL was assessed through EQ – VAS at baseline and after 1 year. <h3>Results</h3> 132 patients completed the study. 5 patients (3.5%) withdrew from the study because of G-I complications, 3 patients (2.1%) did not repeat the DXA. A statistically significant increase of BMD was noted in the lumbar spine, (4.7%, p&lt;0.001) and in the hip region (2.9%, p&lt;0.001). Patients9 perceived health also improved on a Visual Analogue Scale. Median 74 IR 60: 89 compared to median 70 IR 51:86 at the beginning of the study. <h3>Conclusions</h3> Oral Ibandronate is safe and effective in increasing BMD in both Hip and lumbar spine. Apart from reducing the risk of fractures, it helps to improve the patients9 perceived Health Related Quality of Life. <h3>Disclosure of Interest</h3> None declared
Characterisation of Three Unique Head-to-Tail Alpha Globin Cluster Duplications Contributing to Beta Thalassemia Intermedia in 3 Families
Authors: Barnaby E. Clark, Claire C. Shooter, Frances Smith, David Brawand, Laura Steedman, Matthew Oakley, Peter Rushton, Helen Rooks, Xunde Wang, Anthi Drousiotou, Andriani Kyrri, Xenia Felekis, Miranda Petrou, Andrew M Will, Chris Fisher, Douglas R. Higgs, Cornelius Harteveld, Marion Phylipsen, Marina Kleanthous, Swee Lay Thein
Journal: Blood (2015)
PWE-157 EUS Guided Trans-Gastric Pancreatic Pseudocyst Drainage- Does Standard Use of Fluoroscopy Just Complicate Things?: Abstract PWE-157 Table 1
Authors: JA Campbell, AD Hopper
Journal: Gut (2016)

<h3>Introduction</h3> Endoscopic pancreatic pseudocyst drainage is effective and associated with lower morbidity and mortality compared to surgical or percutaneous methods. EUS guidance is now the gold standard intervention due to a higher success rate and lower complication rate compared to blind puncture with fluoroscopic assistance. Many centres still use fluoroscopic guidance with EUS for all cyst-gastroscopy procedures. We present single centre experience of EUS guided pseudocyst drainage and complication rates comparing complication rates with and without fluoroscopic assistance. <h3>Methods</h3> All EUS guided trans-gastric and trans-duodenal pseudocyst and acute pancreatic fluid collections drainage procedures from 2010 to January 2016 were included. Demographics, use of fluoroscopy and initial success of drainage with stent placement were recorded. All procedures were performed with midazolam and fentanyl sedation without endotracheal tube insertion. Standard trans-gastric drainage was attempted if there was good luminal/gastric wall apposition (cyst distance &lt;10 mm) with no interposing vessels. Drainage was performed with 19 g needle puncture with fluid aspiration, 0.035 inch guide wire insertion, followed by 10 French cystotome puncture with ‘endocut’ effect 3 diathermy. A second wire was placed down the cystotome and subsequently, two 4cm 7 French double pigtail stents were placed over each wire. Balloon dilatation to 10 mm of the tract prior to stent placement was performed if the aspirated fluid was purulent or infection suspected. Success of drainage and immediate and delayed complications were identified from patient records. Complication rates were calculated separately for procedures performed with fluoroscopy and those without. The two groups were then compared (Fishers test). <h3>Results</h3> 71 procedures were performed in 68 patients (mean age 50.8, SD 16.1). Immediate drainage was achieved in 69/71 (97.1%) of cases (2 procedures were abandoned due to bleeding). 2 procedures used fully covered metal stents, the remainder used two plastic stents. 2 procedures were performed via the duodenum due to cyst position. 9 patients experienced complications. Although the complications in the fluoroscopy group were more frequent there was no significant statistical difference in complication rates on comparing the two groups (p = 0.25). <h3>Conclusion</h3> Although fluoroscopy could be helpful in certain circumstances, the routine additional use of fluoroscopy does not reduce complications for EUS guided trans-gastric drainage of pancreatic pseudocysts. <h3>Disclosure of Interest</h3> None Declared
Cytogenetic and Molecular Genetic Aberrations in Bone Marrow Mesenchymal Stroma Cells from Patients with Myelodysplastic Syndrome and Acute Myeloid Leukemia.
Authors: Olga Blau, Wolf-Karsten Hofmann, Igor W. Blau, Claudia D. Baldus, Gundula Thiel, Verena Nowak, Elke Schuemann, Annette Sindram, Korinna Kuehne, Eckhard Thiel
Journal: Blood (2007)
DNA and All-Trans Retinoic Acid as Immunotherapy or Add-on Adjuvants to 5-Azacytidine In Myelodysplastic Syndromes
Authors: Carole Le Pogam, Patricia Krief, Stéphanie Beurlet, Murielle Reboul, Robert West, Marika Pla, Dominique Charron, Pierre Fenaux, Christine Chomienne, Rose Ann Padua
Journal: Blood (2010)
Disulfiram Combined with Copper Induces Acute Myeloid Leukemic Stem-like KG1a Cell Apoptosis through TNF-a/ROS Pathway
Authors: Bing Xu, Yong Zhou, Manman Deng, Wenbin Zhong, Kai Chen, Huijuan Dong, Daoguang Yan, Shuyun Zhou
Journal: Blood (2015)
Peptide-Induced Antigen-Specific CD4+CD25+FoxP3+ T Cells Suppress Cytotoxicity T Cell Responses Directed Against the AAV Capsid.
Authors: Daniel J. Hui, Etiena Basner-Tschakarjan, Gary C. Pien, William D. Martin, Annie S. DeGroot, Katherine A. High, Federico Mingozzi
Journal: Blood (2010)
AB0070 A Role of Synovial Exosomes in Osteoclast Differentiation of Inflammatory Arthritis
Authors: J.E. Song, J.H. Shin, K.W. Moon, S.H. Shon, J.S. Park, E.B. Lee, Y.W. Song, E.Y. Lee
Journal: Annals of the Rheumatic Diseases (2016)

<h3>Background</h3> Exosomes are small membrane vesicles (40–150 nm) of endocytic origin secreted by many types of cells and engage in cell-to-cell communication by transferring proteins, microRNAs, mRNA, and lipid to recipient cells. Exosomes are also identified in various biological fluids including blood, urine, amniotic fluid, saliva, malignant ascites, and synovial fluid. Until now, the role of exosomes from synovial fluid (SF) on human osteoclastogenesis is unknown. <h3>Objectives</h3> We aimed to identify the characteristics of synovial exosomes and evaluate the effects on human osteoclastogenesis. <h3>Methods</h3> Synovial exosomes were isolated from SF of rheumatoid arthritis (RA), osteoarthritis (OA), ankylosing spondylitis (AS), and gout patients using ExoQuick (System Biosciences, USA). Monocytes were isolated from human peripheral blood mononuclear cell and were differentiated into macrophages by treatment of 20 ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) for 3–4 days. Then, macrophages were incubated with synovial exosomes in alpha-MEM supplemented with 10% FBS and 1% penicillin/streptomycin for 9–10 days. Osteoclast differentiation was evaluated by tartrate resistant acid phosphatase stain. Exosomes from RA (n=11), OA (n=5), AS (n=6), and gout (n=6) patients were quantified by measuring acetylcholinesterase activity in colorimetric assay. <h3>Results</h3> The number of synovial exosomes isolated from same volume of SF from RA, OA, AS, and gout patients was assessed. We found that exosomes are abundant in SF of RA, AS, and gout patients compared to OA patients. (mean ± SEM 7.36×10<sup>7</sup>±1.35×10<sup>7</sup>, 9.91×10<sup>7</sup>±2.50×10<sup>7</sup>, 7.50×10<sup>7</sup>±2.17×10<sup>7</sup> vs. 2.66×10<sup>7</sup>±0.42×10<sup>7</sup>). Interestingly, the number of exosomes from AS SF was significantly higher (by 3.72 fold, p=0.0476) than exosomes from OA SF. Exosomes from RA, OA, AS, and gout patients induced proliferation and osteoclast formation without M-CSF and receptor activator of nuclear factor kappa-B ligand (RANKL) (Figure 1), which is essential to proliferation and differentiation of osteoclast. Noticeably, exosomes from RA SF had higher potential on osteoclast differentiation than other three diseases. (20.28±14.28 fold vs. 2.38±0.42 fold). <h3>Conclusions</h3> Our results suggest that exosomes from SF of various inflammatory diseases might have differential functional roles in osteoclast differentiation and the number of exosomes may be associated with inflammatory conditions. <h3>References</h3> L. Raimondi et al. Oncotarget 2015 May 30; 6(15): 13772–13789 C Théry et al. Nat Rev Immunol 2002 Aug; 2(8): 569–579 RJ Simpson J et al. Proteomics 2008 Oct; 8(19): 4083–4099 <h3>Disclosure of Interest</h3> None declared
PTU-094 The Assessment of Resting Energy Expenditure in Patients with Cirrhosis Remains Problematic
Authors: C Fennessy, N Kimer, L Greenslade, MY Morgan
Journal: Gut (2016)

<h3>Introduction</h3> Patients with cirrhosis are frequently malnourished and this has a detrimental effect on outcome. Accurate assessment of resting energy expenditure (REE) would facilitate management by providing an objective measure on which to base individualised recommendations for nutritional support. REE can be accurately measured using indirect calorimetry but this technique cannot be used easily in the clinical setting. A number of portable bedside techniques for estimating REE have now been developed although they have not, to date, been validated in this patient population. The aim of the present study was to validate the use of alternative methods for assessing REE against the ‘gold standard’ of indirect calorimetry. <h3>Methods</h3> The study population comprised 19 patients (12 men: seven women; mean [range] age, 60.3 [31–83] yr) with cirrhosis; 11 (57.9%) were adequately nourished, three (15.8%) moderately malnourished and five (26.3%) severely malnourished. REE was measured, under standardised conditions, using a precision, indirect calorimeter (Cortex Metamax 3 B). Additional REE measurements were undertaken, at the same sitting, using: (i) the Medgem Handheld indirect calorimeter; (ii) the Sensewear Pro 3 armband direct calorimeter; and, (iii) the Bodystat Quadscan 4000 bioelectric impedance analyzer. REE was also predicted using the <i>generic</i> Harris-Benedict, Schofield, Mifflin, and Cunningham equations and the <i>disease-specific</i> Müller and Morgan &amp; Madden equations.<sup>1</sup> <h3>Results</h3> The mean (±1 SD) REE measured using the Metamax indirect calorimeter was 1368±456.4 Kcal/24 hr. REE measurments provided by the alternative methods ranged from 1188.1 kcal/24 hr less to 722.6 Kcal/24 hr more than the Metamax values. The Medgem provided the ‘least inaccurate’ REE measurements but values still differed by approximately ±700 Kcal/24 hr. The differences in measurement values between the portable devices and the ‘gold standard’ were inconsistent and bidirectional. Likewise, REE values obtained using the prediction equations ranged from 1018.6 kcal/24 hr less to 1468.3 Kcal/24vhr more than the Metamax values. The <i>generic</i> Mifflin equation provided the ‘least inaccurate’ REE estimates but values still ranged from 887.7 Kcal less to 558.4 Kcal/24 hr more than the Metamax values. <h3>Conclusion</h3> Measurements of REE provided using these bedside techniques and estimated using the predication equations were not sufficiently accurate to be of value, in the clinical setting, either for the assessment of nutritional requirements nor for monitoring over time to assess disease progression or responses to nutritional therapy. <h3>Reference</h3> 1 Madden AM, Morgan MY. Resting energy expenditure should be measured in patients with cirrhosis, not predicted. <i>Hepatology</i> 1999;<b>30</b>:655–664. <h3>Disclosure of Interest</h3> None Declared
Postpartum Venous Thromboembolic Events Among Carriers of Factor V Leiden and/or Prothrombin Gene Variant: A Cohort Study and Updated Systematic Review. Is Prophylaxis Really Required?
Authors: Sarah Louise Dunbar Takach Lapner, Gregoire Le Gal, Genevieve Le Templier, Mark Walker, Graeme N Smith, Phil Wells, Timothy Ramsay, Nicole Langlois, Nancy Carson, Shi Wu Wen, Marc A. Rodger
Journal: Blood (2012)
SP0104 Neutrophils in The Resolution of Inflammation
Authors: M. Herrmann
Journal: Annals of the Rheumatic Diseases (2016)

Neutrophils are often considered being just cells of the first line defense against pathogens. They are endowed with an arsenal of deadly weapons like reactive oxygen species, proteases and anti-microbial peptides. In a cellular process referred to as degranulation neutrophils secrete preformed chemokines and cytokine that recruit and activate further neutrophils as well as other leukocytes. Neutrophils are phagocytes that vigorously ingest and kill bacteria. In addition, they take up several kinds of particular matter including monosodium urate crystals. After strong activation neutrophils eject their chromatin to form pro-inflammatory, chemo-attractive and bactericidal extracellular traps (NETs). These immobilize and kill pathogens and attract further leukocytes. IL8 secretion, neutrophil attraction and NETosis form a self-stimulating feed back loop that initiates and perpetuates inflammation. Important questions are (Q1) How is this potential dangerous vicious circle controlled? (Q2) Which mechanisms are responsible for the resolution of inflammation? When the density of the neutrophils reaches a certain threshold the NETs tend to aggregate (aggNET) and form extended clumps of chromatin that are decorated with proteolytically active serine proteases. The clumps and the proteases sequester and proteolytically inactivate the neutrophil-derived cytokines and chemokines. Thus NETosis and aggNET formation play important roles for the initiation and resolution of neutrophil-driven inflammation, respectively. Christine Schauer, Markus Hoffmann, Luis Munoz &amp; Martin Herrmann <h3>Disclosure of Interest</h3> None declared
PWE-050 Is Sehcat Scanning Necessary? An Analysis of Sehcat Scans and Risk Factors for Bile Acid Diarrhoea in A Series of 262 Patients with Diarrhoea: Abstract PWE-050 Table 1
Authors: B Arms-Williams, P Mylan, C Woodward, J Green, E Jones, M Khan
Journal: Gut (2016)

<h3>Introduction</h3> Bile acid diarrhoea (BAD) is an under-diagnosed cause of diarrhoea and there is a suggestion that up to a third of patients with previously diagnosed diarrhoea-predominant irritable bowel syndrome have BAD diagnosed by a tauroselcholic [<sup>75</sup>selenium] acid (SeHCAT) scan.<sup>1</sup> BAD risk factors include ileal resection (IR), right hemicolectomy (RH), radiotherapy (RT), cholecystectomy and Crohn’s (CD) but it is unclear whether SeHCAT scans add value to these patient groups.<sup>1</sup> This single-centre retrospective study evaluates SeHCAT scans in patients with/without risk factors and their responses to bile acid sequestrants (BAS). <h3>Methods</h3> We retrospectively evaluated the clinical data of all 269 patients who had a SeHCAT scan from February 2011 to December 2014. Patients with cholecystectomy, CD, RH/IR or previous pelvic RT were compared to those without BAD risk factors. Clinic correspondences were evaluated for presenting symptoms and BAD risk factors, treatment with BAS and their tolerance/effectiveness. Response was measured as good or absent according to patient report to physician. Seven cases were excluded due to absence of clinical data. Statistical analyses were calculated for each individual case group versus the control group with no risk factors for BAD. P-values were adjusted with Bonferroni correction. <h3>Results</h3> Average age at time of scan was 53.8. M:F was 1:2.74. 115/262 had BAD confirmed with a SeHCAT scan of &lt;11%. Table 1 shows the scan results for each case group and analyses comparing each to the control group. Three groups were identified as having increased risk of BAD: cholecystectomy, quiescent CD (ileal and colonic) and RH/IR. Colestyramine was trialled in 100 BAD cases, 84% had a good response but 38% tolerated therapy. Colesevelam was trialled in 64 BAD cases, 84% had a good response and 48% tolerated therapy. <h3>Conclusion</h3> We demonstrate that a history of cholecystectomy, CD or RH/IR with chronic diarrhoea is highly suggestive of BAD. Of the six CD patients without BAD, four had isolated colonic disease. Pelvic RT did not increase risk of BAD. A SeHCAT scan can cost £486<sup>2</sup> whilst a month’s trial of either BAS costs &lt;£40<sup>3</sup> and have high response rates in BAD. Given the high probability of a positive scan in cases of RH/IR we suggest trials of empirical BAS could be used instead of SeHCAT for diagnosis in these patients. <h3>References</h3> 1 L Wedlake, <i>et al</i>. <i>AP&amp;T</i> 2009;<b>30</b>:707–717. 2 NICE. Diagnostic guidance DG7, 2012. 3 BNF 2012;<b>64</b>. <h3>Disclosure of Interest</h3> None Declared
Photo-decomposable Organic Nanoparticles for Combined Tumor Optical Imaging and Multiple Phototherapies
Authors: Wenjun Miao, Hyungjun Kim, Vipul Gujrati, Jin Yong Kim, Hyeongsu Jon, Yonghyun Lee, Minsuk Choi, Jinjoo Kim, Soyoung Lee, Dong Yun Lee, Sukmo Kang, Sangyong Jon
Journal: Theranostics (2016)

Combination of photodynamic therapy (PDT) with photothermal therapy (PTT) has achieved significantly improved therapeutic efficacy compared to a single phototherapy modality.However, most nanomaterials used for combined PDT/PTT are made of non-biodegradable materials (e.g., gold nanorods, carbon nanotubes, and graphenes) and may remain intact in the body for long time, raising concerns over their potential long-term toxicity.Here we report a new combined PDT/PTT nanomedicine, designated SP 3 NPs, that exhibit photo-decomposable, photodynamic and photothermal properties.SP 3 NPs were prepared by self-assembly of PEGylated cypate, comprising FDA-approved PEG and an ICG derivative.We confirmed the ability of SP 3 NPs to generate both singlet oxygen for a photodynamic effect and heat for photothermal therapy in response to NIR laser irradiation in vitro.Also, the unique ability of SP 3 NPs to undergo irreversible decomposition upon NIR laser irradiation was demonstrated.Further our experimental results demonstrated that SP 3 NPs strongly accumulated in tumor tissue owing to their highly PEGylated surface and relatively small size (~60 nm), offering subsequent imaging-guided combined PDT/PTT treatment that resulted in tumor eradication and prolonged survival of mice.Taken together, our SP 3 NPs described here may represent a novel and facile approach for next-generation theranostics with great promise for translation into clinical practice in the future.
SP0201 Sport and Exercise for Arthritis Patients
Authors: K. Pfeifer
Journal: Annals of the Rheumatic Diseases (2016)

The positive health enhancing effects of exercise, physical activity (PA), and sport for the general population as well as for persons with musculoskeletal diseases like osteoarthritis or rheumatoid arthritis are indisputable. High potential lies in activating biopsychosocial exercise interventions that target long-term changes of physical activity behavior, and thus think beyond short intervention periods with mostly short-term effects on physical functions. Those behavioral interventions are related to the framework of the International Classification of Functioning, Disability and Health (ICF) of the World Health Organization (WHO) and consider – in addition to the classical target area of restoration of body functions and structures – relevant activities and aspects of participation as well as personal context factors. Based upon recent psychological health behavior change (HBC) theories and using the principles of intervention mapping we identified 28 exercise-related techniques that impact relevant modifiable personal factors of PA. Together with classical exercise components these techniques were integrated into an ICF-based concept of a “Behavioral Exercise Therapy” (BET). This concept serves as a theory-driven “tool box” for designing complex BET programs to promote PA and to build and strengthen individual movement-related health competence. In BET relevant contents and methods are didactically arranged on three intervention levels to enable the development of movement-related health competence: 1) physical exercise and training, 2) learning/education, and 3) experience. Movement-related health competence is composed of the three components movement competence, control competence and self-regulation competence. It is regarded as a personal resource for the movement and mobility demands in daily life, for autonomous health enhancing physical activity and sport, and for coping with or adjustment to health disorders and diseases. <h3>Disclosure of Interest</h3> None declared
Epigenetic Silencing of the RUNX3 Gene by Promoter Hypermethylation in Patients with Acute Myeloid Leukemia.
Authors: Sirisha C Maddipoti, Carlos Bueso-Ramos, Hui Yang, Michael Fernandez, Shaoquing Kuang, Zihong Fang, William Stevenson, Yue Wei, Sherry Pierce, Guillermo Garcia-Manero
Journal: Blood (2008)
Allogeneic Stem Cell Transplantation In Adults With High Risk Acute Lymphoblastic Leukemia (ALL) In 1. Remission (CR1): Promising, But Strongly Influenced By ALL Subtypes
Authors: Renate Arnold, Theis H. Terwey, Giang Lam Vuong, Benjamin Ostendorf, Olaf Penack, Gero Massenkeil, Nicola Gökbuget, Philipp Hemmati
Journal: Blood (2013)
TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells
Authors: Ageliki Tsagaratou, Edahí González-Avalos, Sini Rautio, James P Scott-Browne, Susan Togher, William A Pastor, Ellen V Rothenberg, Lukas Chavez, Harri Lähdesmäki, Anjana Rao
Journal: Nature Immunology (2016)
MM-004: A Phase 1, Dose-Escalation Study to Determine the Maximum Tolerated Dose, Safety, Efficacy, and Pharmacokinetics of Pomalidomide Alone or in Combination with Dexamethasone in Patients with Refractory or Relapsed and Refractory Multiple Myeloma in Japan
Authors: Shinsuke Iida, Hiromi Iwasaki, Takaaki Chou, Kensei Tobonai, Kazutaka Sunami, Yoshiaki Ogawa, Mari Kurihara, Shuichi Midorikawa, Mohamed H. Zaki, Thomas Doerr, Kosei Matsue
Journal: Blood (2014)