tbuckley/cabot-search huggingface dataset:
Abstract 1516: Estrogen regulated TME factors are novel modulators of neovasculogenesis and EMT in breast cancer
Authors: Andrea L. George, Shilpi Rajoria, Arulkumaran Shanmugam, Pradeep Bangalore Prakash, Robert Suriano, Raj K. Tiwari
Journal: Cancer Research (2011)

Abstract The tumor microenvironment plays host to multiple cell types that aid in tumor metastasis and recent work has begun to focus on the impact of non-malignant cells in breast cancer progression. Tumor metastasis relies on neovasculogenesis, aided by bone marrow-derived endothelial progenitor cells (BM-EPCs), which is critical for invasive tumor growth as well as an epithelial to mesenchymal transition (EMT) by malignant cells, allowing them to break from the primary tumor site and migrate to secondary foci. The modulators of tumor induced neovasculogenesis as well as EMT need elucidation. Previously we reported that estrogen significantly enhanced BM-EPC migration to mouse mammary tumor sites in vivo, resulting in an increase in neovasculogenesis and induction of proangiogenic and prometastatic proteins including vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Using in vitro assays, we observed that estrogen leads to stabilization of the transcription factor hypoxia inducible factor 1 α (HIF-1α), a key regulator of hypoxia signaling, in the mouse mammary tumor cell line TG1-1. We observed that estrogen signaling via HIF-1α leads to an increase in VEGF, and conditioned media from TG1-1 cells treated with estrogen leads to a significant increase in human umbilical vein endothelial cell (HUVEC) migration in vitro, an effect abrogated by the anti-estrogen Fulvestrant as well as 3-(5’-hydroxymethyl-2’-furyl)-1-benzylindazole (YC1) a specific HIF-1α inhibitor. While tumor growth induces neovasculogenesis via endothelial cell recruitment, the inflammatory environment created stimulates infiltration of tumor associated macrophages, or TAMs. We further observed that conditioned media from the mouse macrophage cell line RAW264.7 induced EMT in TG1-1 cells, leading to an increase in both the EMT transcription factor TWIST and TG1-1 cell migration. Thus, an inflammatory environment leads to macrophage dependent secretion of pro-metastatic factors, resulting in breast tumor cell EMT and migratory propensity. We conclude that estrogen modulated secreted factors in different cell types influence the tumor microenvironment and have functional consequences on tumor cell growth and metastasis and may provide targets for clinical therapeutic intervention. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1516. doi:10.1158/1538-7445.AM2011-1516
Efficacy of combining targeted therapies cetuximab and dasatinib for resistant salivary gland carcinomas in vitro.
Authors: A. Brohl, K. Steadman, B. Kallakury, J. F. Deeken
Journal: Journal of Clinical Oncology (2010)

5557 Background: Salivary gland tumors are an uncommon and morphologically diverse group of neoplasms that are highly resistant to cytotoxic chemotherapy. Response rates to multi-agent combinations are low, and recent clinical trials using single targeted agents have uniformly failed to show responses to treatment. We explored in vitro combinations of targeted and cytotoxic agents to improve possible treatment options for this resistant disease. Methods: Cell lines representing the common salivary gland carcinoma subtypes of mucoepidermoid (H292) and adenoid cystic (A-253) along with head and neck squamous cell carcinoma cell lines (SCC9 and SCC11a) were exposed for 96 hours of cisplatin, paclitaxel, ixabepilone, cetuximab, and dasatinib. IC25, 50, and 75 dosages for each line were calculated after single agent and combination treatment, and inhibitory, additivity, or synergy from drug combinations was calculated using Chou-Talalay analysis. EGFR expression was measured in each cell line, and cytotoxicity compared to expression level. Results: Comparable sensitivity was seen across the four cell lines from treatment with cisplatin and ixabepilone. Sensitivity to paclitaxel was more variable, with A-253 more than 100-fold resistant compared to H292. Sensitivity to cetuximab was: H292 > A-253 > SCC9 > SCC11a and to dasatinib was: H292 > SCC11a = SCC9 > A-253. Sensitivity to dasatinib and cetuximab correlated with EGFR expression. Combinations of cetuximab or dasatinib with cisplatin or ixabepilone were addititive in the salivary gland cell lines. No inhibitory combinations were found. Interestingly, significant synergy was found by combining dasatinib and cetuximab in the salivary gland cell lines, with Chou-Talalay coefficients of 0.48 and 0.60 in the H292 and A-253 cell lines, respectively. Conclusions: Combined anti-EGFR and anti-src targeted therapies were highly synergistic in treating salivary gland carcinomas in this in vitro study. This combination warrants further clinical study in this highly refractory disease. Author Disclosure Employment or Leadership Position Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Bristol-Myers Squibb
Abstract 332: Analysis of HER gene family status in hepatocellular carcinoma by FISH
Authors: Hui Wen Chen, Pi Chuan Chang, Sen Yung Hsieh, Wen Hui Weng
Journal: Cancer Research (2010)

Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. The incidence rate is much higher in developing countries than in industrialized countries. More than 75% of HCC cases occur in the Far East and Southeast Asia. Gene HER1 was known commonly overexpressed in many cancers including HCC. Overexpression of HER1 would lead to cell proliferation, migration, metastasis, antiapoptosis and angiogenesis. Therefore, HER1 has been widely considered as a predict biomarker in clinical purpose. For other epidermal growth factor receptor (EGFR; HER) family members, for example HER2, HER3, and HER4, except the HER1 and HER2, HER3, HER4 are seldom to be studied in HCC so far. To our knowledge, the role of HER3 play a distinct behavior in the EGFR family, which required heterodimerization with other HER family members, then follow to trigger the signaling transduction and, therefore, result in tumor cell proliferation, migration, invasion, anti-apoptosis, and increase angiogenesis. The high level expression of HER3 has been observed in breast, ovarian, and lung cancer etc. Hence, HER3 protein expression in HCC using immunohistochemistry was also showed significantly related to cell proliferation activity, tumor size, intrahepatic metastasis, portal invasion and carcinoma differentiation. Accordingly, we hypothesized that not only HER1 but also HER3 may play an important role in HCC, and the structure of gene and copy number changes may need further investigated. We study the gene expression level of HER1 and HER3 in six HCC cell lines and twenty paraffin tissue samples using fluorescence in situ hybridization (FISH). Their expression level was also correlated with gene structures and/or gene copy number alterations. Our result showed that the gene expression of HER1 consistent the previous reports. Most notably, the gene HER3 presented either the different level of copy number changes or gene structure alterations in all six cell lines. For, most of the clinical samples only gene structure aberrations could be observed. In addition, both gene HER1 and HER3 were highly expressed in Tong cell line when compared to other HCC cell lines. The FISH results showed the alteration of genes structure and increased copy number of gene HER1 and HER3 in all cell lines. While the study on the clinical HCC samples revealed that the gene structural aberrations were more common than gene copy number alterations. However, further evidence from larger clinical samples is needed to clarify our current findings. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 332.
The Relation between Insulin Sensitivity and the Fatty-Acid Composition of Skeletal-Muscle Phospholipids
Authors: Mark Borkman, Leonard H. Storlien, David A. Pan, Arthur B. Jenkins, Donald J. Chisholm, Lesley V. Campbell
Journal: New England Journal of Medicine (1993)

Insulin resistance and hyperinsulinemia are features of obesity, non-insulin-dependent diabetes mellitus, and other disorders. Skeletal muscle is a major site of insulin action, and insulin sensitivity may be related to the fatty-acid composition of the phospholipids within the muscle membranes involved in the action of insulin.
Case 29-1991
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, William F. McNeely, Betty U. McNeely, Robert L. Kirkman, Judith A. Ferry
Journal: New England Journal of Medicine (1991)

Presentation of CaseA 56-year-old woman was admitted to the hospital because of recurrent intermittent fever after renal transplantation.There was a history of interstitial nephritis that led to chronic renal failure six years earlier. Extracorporeal hemodialysis was begun. Three months before entry a cadaveric renal-transplantation procedure was performed. The patient's postoperative course was complicated by fever, with the temperature rising as high as 38.9°C on the seventh postoperative day. A diagnosis of acute rejection was made. Management of her care included three 500-mg boluses of methylprednisolone by vein and a 12-day course of OKT3 monoclonal antibodies; ganciclovir was administered . . .
BM-06 * MECHANISTIC INVESTIGATIONS OF LEPTOMENINGEAL METASTASIS FROM SOLID TUMORS
Authors: A. Boire, Y. Zou, L. DeAngelis, J. Massague
Journal: Neuro-Oncology (2014)

Leptomeningeal metastasis represents a rare but fatal outcome of disseminated cancer. Although much has been learned through observational studies, retrospective analyses and case reports, understanding of the molecular mechanisms underlying this complication has been hampered by lack of a mouse model. Therefore, our objective was to create an interrogable mouse model that separates the molecular characteristics required for cancer cell access to the leptomeningeal space from those characteristics needed for cancer cell survival within the CSF. To accomplish this, human and mouse cancer cell lines were subjected to multiple rounds of in vivo selection. First, the cells were selected for survival within the leptomeninges: Parental cell lines were directly injected into the CSF via the cisterna magna and tumor cell growth was monitored with bioluminescent imaging. Cancer cells were collected from the basilar meninges and expanded in culture. After three rounds of this selection, the cells were designated “LeptoR3”. Second, the cells were selected for characteristics allowing for cancer cells to access the leptomeningeal space. The triple-selected “LeptoR3” cells were disseminated hematogenously via intracardiac injection. Mice were monitored for development of leptomeningeal metastases with bioluminescent imaging. After development of leptomeningeal mestastasis, these cells were collected, designated “LeptoIC” and expanded in culture. Finally, the Lepto IC, Lepto R3 and parental cell lines were subjected to gene expression profiling by RNASeq. The transcriptomal profiles of these cell lines demonstrate that leptomeningeal metastases employ a distinct array of genes to gain access to the leptomeningeal space and to survive once there. These mouse models represent a powerful tool for the molecular dissection of leptomeningeal metastasis.
Missing Elements of the History
Authors: Larry A. Allen, Amrut V. Ambardekar, Kalpana M. Devaraj, Joseph J. Maleszewski, Eugene E. Wolfel
Journal: New England Journal of Medicine (2014)

A 59-year-old woman with a history of bilateral total hip replacements and a total knee replacement sees her physician for cough, exertional dyspnea, and foot swelling that had developed 2 weeks earlier while she was on vacation in Europe.
Identifying Older Patients With Acute Myeloid Leukemia Who May Be Candidates for Reduced-Intensity Hematopoietic Cell Transplantation
Authors: Boglarka Gyurkocza, Frederick R. Appelbaum
Journal: Journal of the National Comprehensive Cancer Network (2011)

Recent studies show that with reduced-intensity and nonmyeloablative conditioning regimens, allogeneic hematopoietic cell transplantation can now be performed with relative safety in patients with acute myeloid leukemia up to 75 years of age, and therefore chronologic age itself should no longer be considered a contraindication for this procedure. Best results are generally seen in patients undergoing transplant during first remission. Results appear superior to what might be expected with conventional chemotherapy but prospective randomized trials have not been completed. If a decision is made to delay transplant until first relapse, a careful monitoring plan should be established.
Intérêt des biopsies duodénales dans l'exploration de l'anémie ferriprive chez le sujet âgé
Authors: N Ben Chaabane, H Ben Youssef, H Loghmeri, O Hellara, W Melki, F Bdioui, L Safer, H Saffar
Journal: Endoscopy (2011)

Introduction: L'objectif de notre étude est de déterminer l'intérêt des biopsies duodénales systématiques dans le diagnostic de la maladie coeliaque chez les patients âgés ayant une anémie ferriprive dont l'endoscopie digestive n'a révélé aucune cause de la carence en fer.
Abstract 1714: Transcriptional networks downstream of the AR identify clinically relevant prostate cancer targets
Authors: Charlie Massie, Andy Lynch, Rory Stark, Anthony Ramos-Montoya, Ladan Fazli, Anne Warren, Helen Scott, Helene Bon, Naomi Sharma, Vinny Zecchini, Nik Matthews, Michelle Osborne, James Hadfield, Jane Swatton, Stewart McArthur, Boris Adryan, Elena Grigorenko, Carolyn Watt, Iain McEwan, Paul Rennie, David Neal, Ian G. Mills
Journal: Cancer Research (2010)

Abstract The AR signalling axis is central to Prostate Cancer (PrCa) biology, shown by its use in both screening (PSA) and treatment of the disease (androgen deprivation therapy). Identifying direct target genes of the AR will provide a better understanding the key signalling pathways controlled by the AR in PrCa and could lead to improved biomarkers and future therapeutic targets for the disease. To this end we have performed fine mapping of androgen responsive gene expression in prostate cancer cell lines using Illumina bead-arrays, coupled with AR chromatin-immunoprecipitation and Solexa sequencing technology (ChIP-seq). This approach generated over ten thousand candidate AR target genes. We have then undertaken large scale, cross-platform validation using BioTrove Realtime PCR panels which allow the measurement of &amp;gt;600 transcripts simultaneously. Using this combination of approaches we have identified and validated several hundred AR regulated genes. We found that metabolic enzymes and kinases were significantly enriched in the set of direct AR regulated genes. In nine independent clinical gene expression studies we found that the most consistently up-regulated AR target gene was a calcium regulated kinase. At the protein level this kinase showed increased expression in two independent patient cohorts. A small molecule inhibitor of this kinase reduced the growth of a panel of PrCa cell lines in vitro and tumour growth in xenograft models. Interestingly, we found that the levels of this AR regulated kinase were reduced in clinical PrCa following androgen deprivation therapy, but were raised in Castrate Resistant disease. Therefore, we have identified an AR regulate kinase which contributes to tumour growth and is a potential marker and therapeutic target in both hormone naïve and anti-androgen resistant disease. In summary, these data show that combining expression analysis, ChIP-seq and high through-put validation has identified a framework to understand the oncogenic functions of the AR in prostate cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1714.
Stat Bite: Breast Cancer Risk in Prevention Trial Participants
Authors: J. P. Costantino
Journal: JNCI Journal of the National Cancer Institute (1994)

Journal Article Stat Bite : Breast Cancer Risk in Prevention Trial Participants Get access Joseph P. Costantino, Ph.D. Joseph P. Costantino, Ph.D. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 86, Issue 13, 6 July 1994, Page 959, https://doi.org/10.1093/jnci/86.13.959 Published: 06 July 1994
ASSOCIATION BETWEEN PATIENT CHARACTERISTICS AND BASELINE HEALTH STATUS AMONG AN INTERNATIONAL COHORT OF CLAUDICANTS: AN ANALYSIS FROM THE PATIENT-CENTERED OUTCOMES RELATED TO TREATMENT PRACTICES IN PERIPHERAL ARTERIAL DISEASE: INVESTIGATING TRAJECTORIES (PORTRAIT) REGISTRY
Authors: Adam MacArthur Noyes, J. Abbott, Kensey Gosch, Kim Smolderen, John Spertus, Omar Hyder, Peter Soukas, Mehdi Shishehbor, Herbert Aronow
Journal: Journal of the American College of Cardiology (2016)
Duodenal Dieulafoy's Lesion
Authors: Mohamad A Eloubeidi, Nadim Walid El Majzoub
Journal: New England Journal of Medicine (2012)

A 67-year-old man was admitted after 1 day of melena and hematemesis. Thirty years earlier, he had undergone Billroth type I operation (gastroduodenostomy) for treatment of peptic ulcer disease.
EUS-guided pancreaticogastrostomy or pancreaticoduodenostomy for symptomatic patients with pancreatic duct obstruction who are unsuitable or unsuccessful for transpapillary drainage
Authors: Jang Ji Woong, Lee Sang Soo, Park Do Hyun, Seo Dong-Wan, Lee Sung-Koo, Kim Myung-Hwan
Journal: Endoscopy (2011)

Patients with pancreatic duct obstruction, which can be caused by chronic pancreatitis, suffer from recurrent pain. Therefore, the benefit of relieving main pancreatic-duct obstruction has been best studied with chronic pancreatitis. Recently, endoscopic ultrasound-(EUS-) guided pancreaticogastrostomy has been reported as an alternative method for reducing ductal hypertension in patients with chronic pancreatitis. We evaluated our 3-year experience about feasibility and safety of EUS-guided pancreaticogastrostomy or pancreaticoduodenostomy for symptomatic patients with pancreatic duct obstruction who are unsuitable or unsuccessful for transpapillary drainage.
PMO-050 A survey of GP's knowledge and attitudes towards bariatric surgery in Scotland
Authors: J Kynaston, A Mitchell, D Bruce
Journal: Gut (2012)

<h3>Introduction</h3> 25% of Scotland9s population are obese. The benefits of bariatric surgery are established however General Practitioners (GP9s) influence equality of access to this treatment. This study examines GP9s knowledge and attitudes to bariatric surgery in Scotland. <h3>Methods</h3> An electronic questionnaire-based study was emailed to all 902 GP9s within the NHS Highland, Grampian and Tayside health board regions in Scotland. The questionnaire was piloted with a convenience sample of GP9s. Respondents were asked their age, sex and eight questions examining their knowledge, experience and opinions regarding bariatric surgery. <h3>Results</h3> 230 GP9s completed the survey (25.4% response), a further 11 incomplete submissions were not analysed. 60% (n=139) of respondents were female. 93% of GPs acknowledge they often encounter weight management issues. 62% (n=142) of GPs acknowledge bariatric surgery has an important role in weight management and 69% (n=159) acknowledge it9s importance in the management of the obesity related co-morbidities. 30% (n=68) of GPs are not aware of NICE/SIGN guidelines and 57% (n=132) are not aware of their local referral criteria. 40% (n=103) of GPs have never referred. Of those that have, 34% (n=43) of referrals were to the private sector. 76% (n=174) of GPs are not comfortable managing patients who have undergone bariatric surgery. 8% (n=17) of GPs believe the primary role of bariatric surgery is cosmetic. Of these, 47% (n=9) are aged 25–40 years. <h3>Conclusion</h3> Weight management issues are common in primary care and most GP9s support bariatric surgery. However a third of GP9s are unaware of national guidelines and half do not know how to refer to their local service in Northern Scotland. Most GP9s are not comfortable providing long-term care after bariatric surgery. These findings suggest GP9s require education and support to allow their patients access to bariatric surgery. <h3>Competing interests</h3> None declared.
mRNA-electroporated mature dendritic cells retain transgene expression, phenotypical properties and stimulatory capacity after cryopreservation
Authors: P Ponsaerts, VFI Van Tendeloo, N Cools, A Van Driessche, F Lardon, G Nijs, M Lenjou, G Mertens, C Van Broeckhoven, DR Van Bockstaele, ZN Berneman
Journal: Leukemia (2002)
Abstract 1867: Metabolomic discrimination of undifferentiated and differentiated gastric cancer.
Authors: Kenjiro Kami, Tamaki Fujimori, Masanori Terashima, Masatoshi Kusuhara, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Hiroyuki Yamamoto, Yoshiaki Ohashi
Journal: Cancer Research (2013)

Abstract Clinicopathological characteristics of gastric cancer patients strongly depend on histological type. In contrast with gene and protein expressions, metabolic properties of undifferentiated (UT) and differentiated (DT) gastric cancer have been largely unknown. Here, we conducted comparative metabolome analyses in UT and DT cell lines, uncovering characteristic differences between the levels of metabolites involved in energy metabolism. The metabolomic profile of DT cell lines was featured by its considerably high glycolytic intermediate, nucleotide, and glutathione levels, which highlight hallmarks of cancer metabolism represented by the Warburg effect and hyperactive nucleotide syntheses. In contrast, UT cell lines rather showed relatively low energy and redox statuses. We further confirmed the trends by measuring 392 metabolites of paired non-tumor and tumor gastric tissues obtained pairwise from 27 gastric cancer patients in order to metabolomically characterize non-tumors, UT (n=18), and DT (n=9) tumors. The results not only distinguished the profile of non-tumors from tumors, especially of DT, but also reaffirmed the distinctions between UT and DT tumors. Taken together, UT and DT cancers were metabolomically characterized both in vitro and in vivo; DT exhibited more typical cancer metabolic profiles than UT, whereas low glutathione ratios in UT imply their vulnerability to oxidative stress, which thus could be useful for developing more subtype-specific cancer therapeutics. Citation Format: Kenjiro Kami, Tamaki Fujimori, Masanori Terashima, Masatoshi Kusuhara, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Hiroyuki Yamamoto, Yoshiaki Ohashi. Metabolomic discrimination of undifferentiated and differentiated gastric cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1867. doi:10.1158/1538-7445.AM2013-1867
GALECTIN-3 FOR HEART FAILURE RISK STRATIFICATION IN PATIENTS AFTER ACUTE CORONARY SYNDROMES (ACS): INSIGHTS FROM THE SOLID-TIMI 52 TRIAL
Authors: Giulia Magnani, Michelle O’Donoghue, Eugene Braunwald, Dylan Steen, Petr Jarolim, Mary Ann Lukas, Harvey White, Basil Lewis, Robbert de Winter, Jing Zhou, Kyungah Im, Christopher Cannon, David Morrow
Journal: Journal of the American College of Cardiology (2015)
Navigation System for Endoscopic Ultrasonography
Authors: M Kida, H Moriki, H Kikuchi, M Araki, M Takezawa, M Takezawa, H Imaizumi, K Saigenji
Journal: Endoscopy (2006)

It is rather difficult to understand biliopancreas anatomy with EUS and it takes rather long time to do it too. Now we have been collaborated with Olympus to develop a navigation system for EUS and will present about newly developed navigation system.