tbuckley/cabot-search huggingface dataset:
AB1322 Influence of dietary nutrients in prevalence of osteoarthritis (OA) knee
Authors: D. Sanghi, S. Raj, R.N. Srivastava
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> There is growing recognition of the importance of nutritional factors in the maintenance of bone and joint health and that nutritional imbalance combined with endocrine abnormalities may be involved in the pathogenesis of Osteoarthritis (OA). The present study sought to identify influence of dietary nutrients in prevalence of Osteoarthritis (OA) knee <h3>Methods</h3> A cross sectional study - 150 Subjects were recruited from outpatient clinic with the diagnosis of knee Osteoarthritis (KOA) according to the criteria of American College of Rheumatology (ACR). Controls were age sex matched healthy subjects who were free from disease under study. OA was radiologically graded according to Kellgren-Lawrence (KL) grades. Body Mass Index (BMI) was recorded by standard procedure. Dietary nutrient intakes were analyzed by self administered questionnaire including three day dietary recall and food frequency table (FFQ). <h3>Results</h3> An average weight and BMI was significantly higher in subjects with OA knee in comparison to subjects without KOA. Among all dietary factors under study riboflavin, β-carotene, vitamin C and vitamin D was significantly lower in subjects having OA knee in comparison to subjects without OA knee. In unadjusted logistic regression, lower intake quartile of riboflavin, β-carotene, vitamin C and vitamin D having higher risk of OA knee in comparison to higher intake quartiles. However in adjusted analysis, risk of OA knee for riboflavin was diminished and β-carotene lose some strength but vitamin C and D having similar strength of risk of OA knee in quartile having lower intake. As the severity of disease was only defined by vitamin D intake. <h3>Conclusions</h3> The present cross sectional study revealed that lower intake of β- carotene, vitamin C and vitamin D intake is risk factor for knee OA. These nutrients might be an explanatory nutrient for the course of OA knee and might lead disease modifying effect <h3>Disclosure of Interest</h3> None Declared
Abstract P2-10-24: Independent validation of Recurrence Online using 1,638 breast cancer microarray samples
Authors: B Györffy, B Weltz, Z Benke, J Timar, Z Sztupinszki, R Schaefer
Journal: Cancer Research (2012)

Abstract Background: RecurrenceOnline.com uses Affymetrix microarrays to compute the recurrence score and to measure estrogen receptor and HER2-receptor status for breast cancer patients. Our aim was to assess the efficacy of measuring HER2 status as well as the accuracy of survival prediction using an independent set of breast cancer gene expression profiles. Methods: In GEO, we mined new datasets published after the release of Recurrence Online containing detailed clinical characteristics of the profiled cancer patients. The raw microarray data were downloaded and each array was analyzed by running the algorithm available at www.recurrenceonline.com. Then, the subgroups were compared using Kaplan-Meier analysis. For HER2 status determination, the immunohistochemistry (IHC)-based HER2 results were correlated to the microarray results. The cut-off value for HER2 mRNA positivity was set to 4,800. The accuracy was assessed using chi-square tests. Results: Altogether 1,638 new breast cancer samples were analyzed in 9 GEO datasets (these include GSE16446, GSE17907, GSE19615, GSE25066, GSE20711, GSE26971, GSE31448, GSE31519, and GSE20685). The average relapse-free survival among these patients was 4.51 years, 68% of patients were ER positive and 672 were lymph node positive. RecurrenceOnline was capable to classify all patients into good and bad prognosis groups with high accuracy in all patients (p = 2.04E−09), and with p = 1.6E−03 after excluding lymph node positive samples. HER2 IHC data were published for 1,177 patients, with 12% of the patients being positive. HER2 status was correctly assessed for 93% (p &amp;lt; 7E−20) of the patients. Discussion. We verified the classification accuracy of RecurrenceOnline using independent datasets. Our results further support the feasibility of using genome-wide microarrays for patient classification. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P2-10-24.
FRI0085 Social status of adult patients with childhood onset arthritis
Authors: N. Muravieva, Y. Muraviev
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Problems of social rehabilitation of adult patients with childhood onset arthritis are not well studied though very urgent. <h3>Objectives</h3> To assess social status of adult patients with childhood onset arthritis. <h3>Methods</h3> Analysis of database of adult patients with childhood onset arthritis. Activity of the disease (according to DAS28), functional class (FC) according Steinbrocker and social status of patients (education, work activity, marital status) were assessed. <h3>Results</h3> Database contains information on 210 adult patients with childhood onset arthritis: 157 women, 53 men in age from 16-64 (mean 24.6±8.3) years, with disease duration of 16.2±8.7 (1-50) years. Disease activity was found in 85.2% of patients (I – in 127, II – in 43, III – in 9), remission – in 31 (including non-medicamental - in 16 patients). The majority of patients (87.6%) had the II FC, 4 patients move in wheel-chair. Bone fractures were found in 8 patients, joint prosthetics – in 20 patients. Two patients have depraved vision on the level of color distinguishing. At present higher education was finished or in the process of continuation by 46 patients, special education was finished or in the process of continuation by 144 patients. Only school education is peculiar to 9.5% of patients. Out of those with full education (110 patients) 73 are working, while 37 patients are in dependence. 52 patients have family (94% - women), 34 patients (91% - women) have children: one – 25, two – 7, three - 1, six -1. <h3>Conclusions</h3> The majority of adult patients with childhood onset arthritis are socially adapted: 90% have (or continued) education; 66% with full education are working; every 4<sup>th</sup> has family, every 6<sup>th</sup> has a child. At the same time the third of patients do not work are not getting education and, consequently, need social rehabilitation. <h3>References</h3> Packham J.C., Hall M.A. Long-term follow-up of 246 adults with juvenile idiopathic arthritis: social function, relationships and sexual activity. Rheumatology, 2002, 41, 1440-1443. <h3>Disclosure of Interest</h3> None Declared
Cardiovascular effects of leukotrienes in neonatal piglets. Role in hypoxic pulmonary vasoconstriction?
Authors: C W Leffler, J A Mitchell, R S Green
Journal: Circulation Research (1984)

We investigated the effects of exogenous leukotriene D4, synthesis inhibitors, and a leukotriene receptor antagonist upon chloralose anesthetized, mechanically ventilated, neonatal piglets with constant left pulmonary blood flow and upon piglets with uncontrolled pulmonary blood flow. Leukotriene D4 (100-10,000 ng, intravenously) caused dose-dependent increases in peak tracheal pressure, pulmonary vascular resistance, and systemic arterial pressure. In a limited number of experiments, cardiovascular responses to exogenous leukotriene C4 were qualitatively similar but quantitatively less than those to leukotriene D4. Neither treatment with diethylcarbamazine or the lipoxygenase inhibitor nordihydroguaiaretic acid, nor with the leukotriene receptor antagonist, FPL55712, altered any baseline cardiovascular parameter measured, suggesting the absence of any influence of leukotrienes on resting hemodynamics. Hypoxia or hypoxia combined with mild hypercapnia caused pulmonary vasoconstriction. Neither treatment with diethylcarbamazine or the lipoxygenase inhibitor nordihydroguaiaretic acid, nor with the leukotriene receptor antagonist FPL55712, altered the pulmonary vasoconstrictor response to hypoxia or combined hypoxia/hypercapnia. We conclude that endogenous leukotrienes do not appear to have an influence on resting cardiovascular function, neither do they appear to be necessary for hypoxic pulmonary vasoconstriction in the neonatal piglet, although exogenous leukotrienes are capable of producing cardiovascular effects.
Dendritic cell dysfunction and diabetic sensory neuropathy in the cornea
Authors: Nan Gao, Chenxi Yan, Patrick Lee, Haijing Sun, Fu-Shin Yu
Journal: Journal of Clinical Investigation (2016)

Diabetic peripheral neuropathy (DPN) often leads to neurotrophic ulcerations in the cornea and skin; however, the underlying cellular mechanisms of this complication are poorly understood. Here, we used post-wound corneal sensory degeneration and regeneration as a model and tested the hypothesis that diabetes adversely affects DC populations and infiltration, resulting in disrupted DC-nerve communication and DPN. In streptozotocin-induced type 1 diabetic mice, there was a substantial reduction in sensory nerve density and the number of intraepithelial DCs in unwounded (UW) corneas. In wounded corneas, diabetes markedly delayed sensory nerve regeneration and reduced the number of infiltrating DCs, which were a major source of ciliary neurotrophic factor (CNTF) in the cornea. While CNTF neutralization retarded reinnervation in normal corneas, exogenous CNTF accelerated nerve regeneration in the wounded corneas of diabetic mice and healthy animals, in which DCs had been locally depleted. Moreover, blockade of the CNTF-specific receptor CNTFRα induced sensory nerve degeneration and retarded regeneration in normal corneas. Soluble CNTFRα also partially restored the branching of diabetes-suppressed sensory nerve endings and regeneration in the diabetic corneas. Collectively, our data show that DCs mediate sensory nerve innervation and regeneration through CNTF and that diabetes reduces DC populations in UW and wounded corneas, resulting in decreased CNTF and impaired sensory nerve innervation and regeneration.
Chronic diarrhea because of villous atrophy unrelated to celiac disease
Authors: Anja van Lent, R. Takkenberg, Susanne van Eeden, Chris Mulder, Ulrich Beuers
Journal: Endoscopy (2015)

A 71-year-old woman was admitted with a 7-month history of watery diarrhea, which had led to an unintentional 30 kg (27 %) of weight loss and admittance to the intensive care unit (ICU) on two separate occasions as a result of dehydration. Endoscopy and video capsule evaluation revealed villous atrophy of the entire small bowel, with fissuring, nodularity, and loss of folds, as shown for both duodenum ([Fig. 1 a]) and ileum ([Fig. 1 b]). The mucosa appeared fragile, with ulcers after biopsies ([Fig. 1 c]). Histology confirmed complete villous atrophy and showed lengthened regenerative crypts, only a few intraepithelial lymphocytes, and thickening of the basal membrane in both proximal ([Fig. 2]) and distal small-bowel biopsies. Colonoscopy revealed a pale and edematous mucosa with superficial ulcerations, more pronounced distally ([Fig. 3]). Microscopic evaluation showed subtle inflammation in colon biopsies with focal erosion, a focally thickened basal membrane, and some apoptotic cells in the epithelium ([Fig. 4 a, b]). Infectious, ischemic, and malignant disorders were excluded. Serum anti-tTG IgA and anti-gliadin IgG were negative during and after gluten exposure, ruling out celiac disease. The clinical presentation and diagnostic findings were most compatible with adult-onset autoimmune enteropathy [1], affecting an extensive part of the digestive tract (stomach to rectum). Immunosuppressive therapy was started; however high dose prednisolone, increasing doses of azathioprine, and immunoglobulins failed to induce any clinical response. The patient continued to produce voluminous diarrhea; however 3 weeks after starting therapy with oral budesonide (3 × 3 mg daily, pulverized in the morning, granules at noon, capsule at night) [1], the patient recovered, with formed stools, clinical improvement, and weight gain. Duodenal biopsies revealed completely restored villous architecture ([Fig. 5]). The patient has remained well for 20 months of follow-up.
Abstract 2493: Capsaicin, a novel radiosensitizer, acts via a TRPV6 mediated mechanism
Authors: Natalie A. Venier, Alexandra J. Colquhoun, Hiroshi Sasaki, Andrew D. Loblaw, Neil E. Fleshner, Laurence H. Klotz, Vasundara Venkateswaran
Journal: Cancer Research (2011)

Abstract Radiosensitizing agents sensitize cells to the lethal effects of ionizing radiation (IR). This permits use of lower doses of radiation to achieve equivalent cancer control thereby minimizing adverse effects to normal tissues. Given their lack of toxicity compounds occurring naturally in the diet make ideal potential radiosensitizing agents. Capsaicin, a compound found in the Capsicum sp. of plants, is a widely consumed food additive in areas with low PCa incidence. Traditionally capsaicin is used to treat chronic pain syndromes; however, recently evidence using in vitro PCa models describes its anti-carcinogenic potential. The transient receptor potential vanilloid-receptor (TRPV)-1 and TRPV6 cation selective channels are thought to be partly responsible for mediating these effects. TRPV-1 and TRPV-6 expression is up-regulated in PCa tissue correlating directly with increasing tumor grade. This suggests TRPV1 and/or TRPV6 may be potential therapeutic targets for capsaicin mediated interventions in PCa patients. As IR and capsaicin both promote apoptosis and inhibit cell cycle progression in vitro we hypothesize an at least additive effect of combining these two therapies. Using clonogenic assays we assessed the effect of ionizing radiation (1-8 Gy) and/or capsaicin (1-10μM) on colony formation rates in 4 human PCa cell lines (LNCaP, PC3, PC3AR2, DU145). Proliferative, apoptotic, TRPV-6 protein markers were assessed using Western blot analyses. Exposure of cells to capsaicin (1-10μM) or IR (1-8Gy) caused significant dose-dependent inhibition of colony formation (p&amp;lt;0.001). Combining capsaicin with IR resulted in further significant inhibition of colony formation rates (P&amp;lt;0.001). Western blot analyses showed LNCaP cells treated with capsaicin and/or IR to have increased expression of pro-apoptotic proteins BAX and Bad, tumor-suppressor proteins p21 and p27 and reduced androgen-receptor. Additionally, capsaicin monotherapy caused a dramatic alteration in TRPV1 and TRPV6 expression. These studies confirm the radiosensitizing capacity of capsaicin in PCa cells in vitro. Ongoing studies using are further delineating the mechanism of interaction of these treatment modalities. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2493. doi:10.1158/1538-7445.AM2011-2493
Eculizumab in Pregnant Patients with Paroxysmal Nocturnal Hemoglobinuria
Authors: Richard J. Kelly, Britta Höchsmann, Jeff Szer, Austin Kulasekararaj, Sophie de Guibert, Alexander Röth, Ilene C. Weitz, Elina Armstrong, Antonio M. Risitano, Christopher J. Patriquin, Louis Terriou, Petra Muus, Anita Hill, Michelle P. Turner, Hubert Schrezenmeier, Regis Peffault de Latour
Journal: New England Journal of Medicine (2015)

Eculizumab, a humanized monoclonal antibody against complement protein C5 that inhibits terminal complement activation, has been shown to prevent complications of paroxysmal nocturnal hemoglobinuria (PNH) and improve quality of life and overall survival, but data on the use of eculizumab in women during pregnancy are scarce.
New Mechanisms of Bacterial Resistance to Antimicrobial Agents
Authors: Franklin H. Epstein, George A. Jacoby, Gordon L. Archer
Journal: New England Journal of Medicine (1991)

Sooner or later bacteria develop resistance to virtually any antimicrobial agent. Resistance has many consequences. The patient infected with a drug-resistant as opposed to a drug-sensitive organism is more likely to require hospitalization and have a longer hospital stay and has an increased risk of death.1 Resistance also compels the use of more toxic or more expensive alternative drugs.2 The unrecognized cost of antibiotic resistance in the United States has been estimated as at least $100 million annually.3 Resistance thus affects the antibiotic options available to every practitioner and is no less a problem in the developing world.4 The evaluation . . .
Quantitative Lung Perfusion Scintigraphy in Postoperative Evaluation of Congenital Right Ventricular Outflow Tract Obstructive Lesions
Authors: JONG HO KIM, DONG SOO LEE, JUNE-KEY CHUNG, MYUNG CHUL LEE, YEON WOO KIM, YONG SOO YUN, KYUNG MO YEON, CHANG-SOON KOH
Journal: Clinical Nuclear Medicine (1996)

Improved methods to relieve pulmonary artery stenosis have emphasized the need for an accurate noninvasive method to quantify the pulmonary blood flow. The aim of this study was to compare the roles of Tc-99m MAA perfusion lung imaging, chest x-ray, MRI, and cine-angiography for postoperative evaluation of pulmonary blood flow in patients with congenital right ventricular outflow tract (RVOT) obstructive lesions. Eleven patients underwent chest x-ray, MRI, perfusion lung imaging, and cine-angiography after corrective surgery of RVOT obstruction. The perfusion ratio on lung scans was calculated on the posterior views. The correlation coefficient of the left to right perfusion ratio measured by lung scans obtained with that by visual assessment of chest x-rays, with the most stenotic diameter ratio on MRI and on angiography being 0.57, 0.88 (P < 0.001), and 0.87 (P < 0.001), respectively. Follow-up perfusion lung scans in seven patients, before and after pulmonary artery intervention showed significantly improved perfusion ratios (0.30 ± 0.11 to 0.70 ± 0.22; P < 0.001), which were concordant with the clinical findings. The authors conclude that the quantitative perfusion lung scan is the noninvasive method of choice for postoperative evaluation of the pulmonary artery in congenital RVOT obstruction.
The Use of Curare in Anesthesia
Authors: Edward C. Allen, Urban H. Eversole
Journal: New England Journal of Medicine (1947)

FOR the past few centuries, curare has been dismissed lightly as being synonymous with the generic name of several arrow poisons of certain South American Indians, but investigation of its pharmacologic action and clinical application in the past decade has shown this drug to hold great promise to the anesthesiologist. The first descriptions of this so-called "arrow poison" were brought back to the civilized world by Sir Walter Raleigh in 1595 following his journey to Guiana and up the Orinoco.1 Because of the variable source and potency of the drug and because of the toxic effects due to the impurities . . .
Simultaneous Splenic and Colonic Metastases From Gastric Cancer
Authors: Sung Hoon Kim, Kyoung Sook Won, Ilseon Hwang, Byung Wook Choi, Il Jo, Seok Kil Zeon
Journal: Clinical Nuclear Medicine (2012)

We describe the ¹⁸F-FDG PET/CT findings of 2 simultaneous metastatic gastric cancers to the colon and spleen with quite different FDG avidities. Longitudinal and weak FDG uptake was observed in the descending colon; therefore, it could not be distinguished from nonpathological functional uptake of normal colon. Meanwhile, the splenic mass showed intense uptake. Contrast-enhanced CT revealed a segmental wall thickening of the descending colon as well as a hypoattenuating splenic mass suggesting metastases. Splenectomy and left hemicolectomy were performed. Histological diagnosis disclosed metastatic gastric cancer. The differential diagnoses and review of the literature are presented.
626 OSCILLATIONS IN SERUM FERRITIN ASSOCIATED TO ANTIVIRAL THERAPY IN CHRONIC HEPATITIS C
Authors: J.M. Ladero, G. López-Alonso, M.J. Devesa, F. Cuenca, J. Ladero, A. Suárez, P. Ropero, M. Díaz-Rubio
Journal: Journal of Hepatology (2009)
Abstract 2017: Exome sequencing reveals novel mutations in a GIST and a paraganglioma occurring simultaneously in a patient with complete form of Carney triad.
Authors: Florian Haller, Abbas Agaimy, Evgeny A. Moskalev, Natalie Jäger, Benedikt Broers, Stefan Wiemann, Arndt Hartmann
Journal: Cancer Research (2013)

Abstract Carney triad is a rare syndrome which is defined by the occurrence of multicentric gastric GIST, paraganglioma and pulmonary chondroma. In contrast to the closely-related Carney-Stratakis syndrome (CSS), Carney syndrome is not inherited. In both syndromes, the tumors are characterized by a loss of Succinate Dehydrogenase B protein (so-called SDHB-deficient tumors). Only recently, inactivating mutations in the SDH subunits A, B, C, and D have been found to be present in the majority of SDHB-deficient GISTs of CSS, but not in GISTs in the setting of Carney triad. We performed exome sequencing of DNA samples derived from blood as well as of the GIST and the paraganglioma from a female patient with a complete Carney triad. Additionally, the genome from the blood DNA was sequenced at high coverage, and low coverage genomes of both tumor DNAs were evaluated for copy number variations. SDHA and B mRNA and protein expression were analysed by qRT-PCR and Western Blot analysis, respectively. Both the GIST and the paraganglioma from the Carney patient were negative for SDHB by immunohistochemistry, and did not display noticeable levels of SDHB in the Western Blot analysis. In contrast, SDHB mRNA levels were equal to SDHA levels, and also to GISTs from sporadic GISTs with KIT mutations. Exome sequencing revealed 10 and 18 somatic missense mutations in the DNA from the GIST and the paraganglioma, which were not present in the DNA from the blood. Additionally, one somatic insertion/deletion was found in each tumor. These novel mutations will provide better insights in our understanding of this rare albeit fascinating disease, and might also be suitable as targets for future therapies. Citation Format: Florian Haller, Abbas Agaimy, Evgeny A. Moskalev, Natalie Jäger, Benedikt Broers, Stefan Wiemann, Arndt Hartmann. Exome sequencing reveals novel mutations in a GIST and a paraganglioma occurring simultaneously in a patient with complete form of Carney triad. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2017. doi:10.1158/1538-7445.AM2013-2017
Proliferative Myositis: A Case of a Pseudomalignant Process
Authors: MIEKE GYSEN, SIGRID STROOBANTS, LUC MORTELMANS
Journal: Clinical Nuclear Medicine (1998)

A case of proliferative myositis, an unusual inflammatory pseudotumor of a skeletal muscle, is presented. Often it is erroneously misdiagnosed as a malignant neoplasm.
Effect of Endotoxin on Granulopoiesis and Colony-Stimulating Factor
Authors: Peter Quesenberry, Alec Morley, Frederick Stohlman, Kevin Rickard, Donald Howard, Marianne Smith
Journal: New England Journal of Medicine (1972)

To study the regulation of granulopoiesis, we measured the effect of endotoxin on peripheral leukocyte counts and on colony-stimulating factor (CSF) in the serum of CF1 mice. The peripheral granulocyte level fell from 612 ± 69 (mean ± S.E.M.) to 147 ± 20 per cubic millimeter within 45 minutes of intraperitoneal injection of 5 μg of Salmonella typhosa endotoxin. In control animals, the CSF in 0.1 ml of serum stimulated the growth of 0.56 ± 0.4 in vitro myeloid colonies per 105 cells. Forty-five minutes after endotoxin the CSF activity had increased to 7.7 ± 6.1 colonies per 105 cells; after two hours it was 29.6 ± 10 colonies per 105 cells. Six hours after endotoxin the marrow was depleted of polymorphonuclear cells. Thereafter, sequential increases in the myeloblast-promyelocyte and myelocyte compartments of the marrow at 24 to 48 hours, respectively, suggested a wave of differentiation from a precursor compartment with subsequent maturation. The data suggest that intermittent endotoxemia may be one determinant of serum CSF levels, which, in turn, may play a part in regulating granulopoiesis.