tbuckley/cabot-search huggingface dataset:
Abstract 3489: A novel monoclonal antibody to native HER2 epitope detects HER2-positive CTCs, HER2 in blood, and HER2 in tissue: realistic approach for monitoring breast carcinoma.
Authors: Özge Alper, Achille Akoa, Özgül M. Alper, Cui P. Chen
Journal: Cancer Research (2013)

Abstract Background: Amplification of the HER2 gene and/or overexpression of its protein product have been reported in 25% to 30% of breast carcinomas and have been associated with poorer outcomes. However, there are still highly controversial aspects of HER2 testing. In the present study, we generated a novel monoclonal antibody against human native epitope of HER2 and investigated its clinical potential in managing breast carcinoma. Methods and findings: We generated a novel monoclonal antibody (mAb), AB-HER2 using hybridoma technology and native folding of the protein as an immunogen. This mAb is of IgG1 (kappa) type. It specifically recognized both native and denatured human HER2 protein and displayed high affinity as assessed by Circulating Tumor Cells (CTCs), ELISA, immunohistochemistry assay, western blot and flow cytometry applications. We have developed a CTCs assay to detect specific HER2-positive tumor cells circulating in blood, while we did not observe any background staining in blood cells. Using AB-HER2 mAb in ELISA, we showed soluble HER2 level is significantly increased in breast cancer patients’ plasma which directly correlated with HER2 expression pattern in breast cancer tissues. Tissue expression of HER2 was determined by using immunohistochemical techniques. Most of normal tissues showed negative staining, while different levels of membrane staining were observed in cancer sections. To evaluate the immunoreactivity of HER2 associated with tumor histological types, the staining intensity in membranes were scored according to the guidelines of ASCO/CAP. We also have compared AB-HER2 mAB with commercial HER2 antibodies in all immunoassays. Conclusion: The novel AB-HER2 mAb showed improved accuracy in all immunoassays and clinical outcome. Circulating HER2-positive tumor cells, HER2 protein levels in plasma and in breast tissues significantly increased in patients with breast carcinoma compared to normal healthy controls. Our data suggest the potential clinical use of AB-HER2 mAB as a powerful immunoreagent in measuring real-time HER2 levels in managing breast cancer. Citation Format: Özge Alper, Achille Akoa, Özgül M. Alper, Cui P. Chen. A novel monoclonal antibody to native HER2 epitope detects HER2-positive CTCs, HER2 in blood, and HER2 in tissue: realistic approach for monitoring breast carcinoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3489. doi:10.1158/1538-7445.AM2013-3489
OC-009 Withdrawal of anti-tnf following sustained remission for inflammatory bowel disease: a systematic review and meta-analysis
Authors: NA Kennedy, JO Lindsay, J Gordon, A Hart, S McCartney, P Irving, J Satsangi, CW Lees
Journal: Gut (2015)

<h3>Summary</h3> HIV-Exposed Uninfected (HEU) infants are a rapidly expanding population in sub-Saharan Africa and are highly susceptible to disease caused by encapsulated bacteria in the first year of life. The mechanism of this increased risk is still poorly understood. We therefore investigated if HIV exposure dysregulates HEU infant immunity and if this is amplified by human herpes virus infection (HHV). Here, we compared monocyte enzymatic function, innate and adaptive immune cell phenotype, and vaccine-induced antibody responses between HEU and HUU infants. We demonstrate altered monocyte phagosomal function and B cell subset homeostasis, and lower vaccine-induced anti-<i>Haemophilus influenzae type b</i> (<i>Hib)</i> and anti-Tetanus Toxoid (TT) IgG titers in HEU compared to HUU infants. There was no difference in the prevalence of HHV infection between HEU and HUU infants. Our findings suggest that even in the era of antiretroviral therapy (ART)-mediated viral suppression, HIV exposure dysregulates monocyte and B cell function during a vulnerable period of immune maturation in infancy. This may contribute to the high rates of invasive bacterial disease and pneumonia in HEU infants.
Abstract 4739: Mesenchymal stem cells induce broad spectrum resistance to chemotherapy through the release of platinum-induced fatty acids
Authors: Jeanine M.L Roodhart, Laura G. Daenen, Edwin C. Stigter, Kenji Ishihara, Rene H. Medema, Arjan B. Brenkman, Emile E. Voest
Journal: Cancer Research (2011)

Abstract Background The development of resistance to chemotherapy is a major obstacle for lasting effective treatment of cancer. It is becoming increasingly clear that the tumor micro-environment plays a key role in the development of drug resistance. Mesenchymal stem cells (MSCs), key players in tissue repair and wound healing, are recruited in large numbers to the stroma of developing tumors where they are known to support tumor growth and enhance metastasis formation. Aim To determine whether MSCs confer resistance to chemotherapy, we established different murine tumor models in which we resembled the mobilization and recruitment of MSCs from the bone marrow to tumor. Results Administration of 50,000 MSCs intravenously (iv), or as little as 1,000 MSCs subcutaneously (sc) together with chemotherapy almost completely abolished the anti-tumor effect of the chemotherapy in a dose-dependent matter. Notably, this was only observed with the platinum-based chemotherapeutics, namely cisplatin, oxaliplatin and carboplatin. To show that the MSCs induce resistance in a paracrine fashion, we incubated cultured MSCs with platinum-based chemotherapy and injected the conditioned medium (CM+) from these cells sc concomitantly with the chemotherapy. Strikingly, the CM+ induced an almost complete resistance to multiple types of chemotherapy, including non-platinum based chemotherapeutics like 5-FU and irinotecan. The CM from untreated MSCs (CM-), did not induce resistance. Through a metabolomics approach, we identified two unique platinum-induced polyunsaturated fatty acids (PIFAs), 12-oxo-5,8,10-heptadecatrienoic acid (KHT(n-6)) and hexadeca-4,7,10,13-tetraenoic acid (16:4(n-3)) in the CM+ responsible for the induction of resistance by the MSCs. We tested the purified PIFAs in our mouse models and confirmed that in minute quantities (2pmol) these PIFAs induce resistance to a broad spectrum of chemotherapy. In contrast, other related poly-unsaturated fatty acids had no effect. Conclusion In summary, our study identifies a novel paracrine mechanism of resistance via activation of MSCs by platinum-based chemotherapy with the subsequent release of two specific resistance-inducing PIFAs. Our findings suggest that interventions directed against either MSC or the identified PIFAs may be explored to enhance the efficacy of chemotherapy and prevent the development of resistance. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4739. doi:10.1158/1538-7445.AM2011-4739
437 THE IMPACT OF LONGITUDINAL ASSESSMENT OF LIVER STIFFNESS BY TRANSIENT ELASTOGRAPHY FOR CHRONIC HBeAg(−) PATIENTS TREATED WITH NUCLEOS(T)IDE ANALOGUES
Authors: G. Kachagias, G. Zacharakis, E. Athanasopoulos, J. Anastasiou, E. Saprikis, N. Viazis, D. Karamanolis
Journal: Journal of Hepatology (2013)
Gamma-A Deficiency and Hypochromic Anemia Due to Defective Iron Mobilization
Authors: Ingeborg Krieger, Joseph A. Brough
Journal: New England Journal of Medicine (1967)

AN unusual defect in iron metabolism was demonstrated in a patient, who showed the clinical and histologic features of idiopathic pulmonary hemosiderosis.1 Iron kinetic studies indicated that iron was lost into a site where it could not be reutilized for hematopoiesis, causing hypochromic anemia and reticulocytosis. "Iron trapping" in the lung,2 presumably because of fibrosis and lymphatic obstruction,3 , 4 is characteristic of idiopathic pulmonary hemosiderosis. However, the findings presented below suggest that the defect in iron mobilization may be a generalized dysfunction located in the macrophage system, both in this case and in other cases of this condition. It will be . . .
Apparent Intrathoracic Spleen Associated With Marked Scoliosis
Authors: PATRICIA P. PACHECO, EUGENE D. SILVERMAN
Journal: Clinical Nuclear Medicine (1996)

A 73-year-old woman with a history of mitral regurgitation underwent a MUGA scan for evaluation of left ventricular function. The spleen is displaced superior to the left ventricle in an apparent intrathoracic location. A subsequent bone scan revealed that these relationships are due to severe kyphoscoliosis with elevation of the left hemidiaphragm.
Abstract 1261: A mesodissection system for accurate, rapid, and economical recovery of tissue from slide mounted tissue sections
Authors: Nils B. Adey, Robert J. Parry, Lilia Birch, Christian N. Paxton, Siavash Ghaffari, Mark G. Herrmann, Katherine B. Geiersbach
Journal: Cancer Research (2012)

Abstract We have developed a prototype system for slide mounted tissue dissection capable of resolution between manual methods (i.e. scraping tissue off of microscope slides) and laser capture microdissection (LCM); here we test the performance of this system. The mesodissection instrument employs a specialized milling tip that simultaneously dispenses and aspirates liquid in order to capture tissue fragments displaced from the slide surface. The accompanying software is capable of transferring electronically indicated target regions between neighboring tissue sections to guide the dissection process. Resolution and accuracy of the system were accessed by microscopic inspection of the dissected region; resolution is the smallest recoverable area and accuracy is the difference between the actual dissected region and the desired region. Purity is defined as the percentage of desired tissue retrieved from a FFPE tissue block made from a human tumor xenografted into mouse, and accessed using real-time PCR amplification of species-specific repetitive DNA elements, as well as unique genomic regions. Efficiency is defined as total DNA recovered relative to manual methods, as quantified by PicoGreen fluorescence. Currently, the system is capable of 0.3 mm resolution and accuracy better than 0.1 mm compared to a desired boundary. Purity is greater than 90% at 0.1mm from the mouse-human tissue boundary. Efficiency is comparable to manual methods when virtually all tissue is recovered from the region of interest. Improvements to the current prototype are ongoing with the goal of less than 0.1 mm resolution. The mesodissection system provides an inexpensive alternative to the limited resolution of manual dissection methods and the high cost and low recovery efficiency of LCM. Digitally targeted dissection using tissue sections derived from adjacent portions of a tissue block allow for determination of a region of interest from a slide optimized for visualization and recovery from a slide optimized for dissection. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1261. doi:1538-7445.AM2012-1261
Abstract 2192: Functional genomic screen to identify candidates capable of modulating invasive properties of HNSCC
Authors: Tatiana N. Lepikhova, Misa Imai, Nina Peitsaro, Juha Klefstrom, Outi M. Monni
Journal: Cancer Research (2010)

Abstract Head and neck squamous cell carcinomas (HNSCC) contain a heterogeneous population of cancer cells some of which is explained by ongoing mutations that occur due to genetic instability and environmental factors. Not all of the cancer cells have a similar ability to drive tumor formation. To identify genes capable of modulating invasive properties in head and neck squamous cell carcinoma (HNSCC), we have compared copy number profiles (244K aCGH) of 12 primary and metastatic cancer cell lines obtained from patients with HNSCC of tongue and larynx. A specific pattern of copy number gains and losses was present in each cell line studied. Majority of the changes occurred in both the primary tumor and corresponding metastasis, but some amplifications, such as 10q21.1., 11q22, and 17p12 occurred more frequently in metastatic cancers. To determine whether any genes located at the amplicons input to metastatic potential of HNSCC we are performing a systematic loss-of-function survey. First, we examined the cell growth and invasive properties of primary and metastatic cell lines in vitro and in vivo. We then set up a genetic screen using highly metastatic cell lines in in vitro invasion assay system. pLKO1 shRNA library targeting genes located at identified amplified regions in invasive cells were transduced into the selected cell lines and transduced cell lines were subjected to transwell invasion assay. Several genes targeted for amplification and capable of modulating invasive properties of HNSCC in vitro have been selected for further validation in vivo. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2192.
AB1270 Diagnostic performance of knee ultrasonography for detecting degenerative changes of articular cartilage
Authors: J. Koski, S. Saarakkala, P. Waris, V. Waris, I. Tarkiainen, E. Karvanen, J. Aarnio
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> To investigate the diagnostic performance of non-invasive knee ultrasonography (US) to detect degenerative changes of articular cartilage using arthroscopic grading as the gold standard. <h3>Methods</h3> 40 adult patients referred to a knee arthroscopy because of knee pain were randomly selected for the study. Before the arthroscopy, knee US was performed and cartilage surfaces at medial and lateral femoral condyles as well as at intercondylar notch area (sulcus) were semi-quantitatively graded from US. Ultrasonographic grading was compared with the arthroscopic Noyes' grading for cartilage degeneration. <h3>Results</h3> Sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic odds ratio for detecting arthroscopic cartilage changes in US varied between 52-83%, 50-100%, 88-100%, 24-46%, and 5.0-13.0, respectively, depending on the site. Correlation of severity of cartilage changes (grades) between US and arthroscopy varied from insignificant to significant depending on the site: at the sulcus area the correlation was highest (<i>r</i><sub>s</sub> =0.593, <i>p</i> &lt;0.001), at the medial condyle also significant (<i>r</i><sub>s</sub> =0.465, <i>p</i> =0.003), and at the lateral condyle non-significant (<i>r</i><sub>s</sub> =0.262, <i>p</i> =0.103). The sum of cartilage grades in all three sites of the femoral cartilage between US and arthroscopy had the highest correlation (<i>r</i><sub>s</sub> =0.655, <i>p</i> &lt;0.001). <h3>Conclusions</h3> Positive finding in US is a strong indicator of arthroscopic degenerative changes of cartilage, but negative finding does not rule out degenerative changes. Non-invasive knee US is a promising technique for screening of degenerative changes of articular cartilage, <i>e.g.</i>, during osteoarthritis. <h3>Disclosure of Interest</h3> None Declared
Positron Emission Tomography in the Diagnosis of Variant Angina
Authors: GILLIAN A. J. JESSURUN, JOAN G. MEEDER, RENE A. TIO
Journal: Clinical Nuclear Medicine (1997)

The diagnosis of coronary vasospasm in chronic vasospastic angina is established by the history of typical nocturnal angina pectoris with rapid relief after sublingual nitroglycerin, concomitant ST-segment elevation on the electrocardiogram and a recent normal coronary angiogram. True vasospasm may subsequently be confirmed on a coronary angiogram, either spontaneously or by provokation with acethylcholine. The authors show a case of acute vasospasm induced by a cold pressor test in a 40-year-old man who had onset variant angina. Transient reduction of regional myocardial perfusion, angina pectoris at rest, and ST-segment elevation resolved after administration of nitroglycerin.
Women's Health Research Expected to Remain a Priority
Authors: B. Duane
Journal: JNCI Journal of the National Cancer Institute (1992)

Journal Article Women's Health Research Expected to Remain a Priority Get access Betsy Duane Betsy Duane Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 84, Issue 23, 2 December 1992, Pages 1776–1777, https://doi.org/10.1093/jnci/84.23.1776 Published: 02 December 1992
Rapid Spontaneous Cerebrospinal Fluid Leak Detected in the Gastrointestinal Tract
Authors: Hong Yun Ma, Papia Sen, Evan G. Stein, Leonard M. Freeman
Journal: Clinical Nuclear Medicine (2013)

There are many causes of cerebrospinal (CSF) leaks. Most cases are secondary to blunt trauma and iatrogenic trauma caused by postoperative sequelae. Occasionally, CSF leakage may occur from nontraumatic or “spontaneous” causes, such as benign intracranial hypertension and “empty sella syndrome.” Mass effect due to an encephalocele or meningocele may also be seen. Radionuclide cisternography is a sensitive method of determining CSF leak when combined with intranasal cotton pledget placement and analysis. We present a spontaneous CSF fluid leak that was detected when scintigraphic activity appeared first in the gastrointestinal tract.
Complementary Clinical Benefits of Coronary-Artery Stenting and Blockade of Platelet Glycoprotein IIb/IIIa Receptors
Authors: A. Michael Lincoff, Robert M. Califf, David J. Moliterno, Stephen G. Ellis, John Ducas, Jeffrey H. Kramer, Neal S. Kleiman, Eric A. Cohen, Joan E. Booth, Shelly K. Sapp, Catherine F. Cabot, James E. Tcheng, J. David Talley, Paul O. Caramori, Jeffrey R. Burton, Thomas A. Kelly, Tom B. Ivanc, Eric J. Topol
Journal: New England Journal of Medicine (1999)

Inhibition of the platelet glycoprotein IIb/IIIa receptor with the monoclonal-antibody fragment abciximab reduces the acute ischemic complications associated with percutaneous coronary revascularization, whereas coronary-stent implantation reduces restenosis. We conducted a trial to determine the efficacy of abciximab and stent implantation in improving long-term outcome.
Abstract 1341: Identification of significant linkage evidence for lethal prostate cancer on chromosome arm 11p15.
Authors: Lisa A. Cannon-Albright, Craig C. Teerlink, Neeraj Agarwal
Journal: Cancer Research (2013)

Abstract We performed genome wide linkage analysis in a set of high-risk prostate cancer pedigrees, each with 3 or more sampled cases whose death certificate indicated a contribution of prostate cancer to death (lethal prostate cancer). All prostate cancer cases were present in the Utah SEER Cancer Registry (pathology confirmed); all “lethal” prostate cancer cases were also required to have a Utah death certificate indicating prostate cancer as a contributing cause of death. A set of markers with no linkage disequilibrium was selected for linkage analysis. We took the intersection of SNP markers from five Illumina genotyping platforms used (550K, 610K, 1M,Omni_express (720k), and Omni_1M), resulting in selection of 301,646 markers. We began with the first marker on each chromosome and then skipped markers until one satisfied all criteria (min 0.1 cM distance, heterozygosity 0.35, R-squared &amp;lt; 0.16 -using HapMap's LD files based on CEPH). This resulted in selection of a set of 25,436 SNP LD-free genome wide markers for linkage. We have performed genome-wide linkage analysis for 21 of the genotyped high-risk lethal prostate cancer pedigrees with the 25,436 selected SNP markers. Genome wide hetLODs showed one significant linkage peak for the recessive model on chromosome 14 (LOD = 4.20). The pedigree with the highest LOD in this region has a +2.69; we are attempting to expand sampling for other pedigrees with linkage evidence in this region. A single pedigree with 16 prostate cancer cases, at least 4 of whom have already died from prostate cancer and were sampled, provided a LOD score = +3.56 on chromosome arm 11p15. An 11p15 haplotype inherited in common from the founder to 6 generations of descendants is shared in at least 9 of the prostate cancer cases, 3 of whom died from prostate cancer. The 1-LOD drop defined support interval for the pedigree linked to chromosome 11 occurs at approximately 34-46 cM and contains 13 currently documented genomic features, several of which may be reasonable candidates for lethal prostate cancer including the gene PRMT3 (arginine methyltransferase 3), DAL-1, NELL1, FANCF (Fanconi anemia, complementation group F), and GAS2 (growth arrest-specific 2). Sequencing of multiple haplotype sharing cases in the pedigree is underway. Citation Format: Lisa A. Cannon-Albright, Craig C. Teerlink, Neeraj Agarwal. Identification of significant linkage evidence for lethal prostate cancer on chromosome arm 11p15. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1341. doi:10.1158/1538-7445.AM2013-1341
ASSESSMENT OF BALLOON-EXPANDABLE TRANSCATHETER HEART VALVE OVERSIZING USING THREE-DIMENSIONAL TRANSESOPHAGEAL ECHOCARDIOGRAPHY SEMI-AUTOMATION VERSUS. DIRECT PLANIMETRY: ALL METHODS ARE NOT CREATED EQUAL
Authors: Omar K. Khalique, Nadira Hamid, Jonathon White, Juan Terre, Ramin Hastings, Tulio Palmerini, Susheel Kodali, Tamim Nazif, Torsten Vahl, Isaac George, Martin Leon, Rebecca Hahn
Journal: Journal of the American College of Cardiology (2016)
Ventilatory Control in Parents of Victims of Sudden-Infant-Death Syndrome
Authors: Philip L. Schiffman, Robert E. Westlake, Teodoro V. Santiago, Norman H. Edelman
Journal: New England Journal of Medicine (1980)

Since a defective ventilatory-control mechanism may have a role in the sudden-infant-death syndrome (SIDS), and hereditary factors influence the degree of ventilatory drive, we measured ventilatory responsiveness to carbon dioxide with and without increased airway resistance in 12 parents of SIDS victims and 12 control parents matched for age and size. Ventilatory response (ΔV̇1/ΔPCO2) and "respiratory drive" (ΔP100/ΔPCO2) were measured both with and without added resistance to inspiratory flow. SIDS parents had significantly lower ventilatory response with added resistance (P<0.05) and without it (P<0.01) and significantly lower respiratory drive with added resistance (P<0.001) and without it (P<0.05). Control parents had significantly increased respiratory drive when the resistance was added (P<0.005), whereas SIDS parents did not. The data suggest that a low ventilatory response to carbon dioxide and a diminished compensatory response to increased airway resistance may increase a potential parent's risk of having a child susceptible to SIDS. (N Engl J Med. 1980; 302:486–491.)