tbuckley/cabot-search huggingface dataset:
Genetic Analysis of a Sarcoma Accidentally Transplanted from a Patient to a Surgeon
Authors: Hermine-Valeria Gärtner, Christian Seidl, Christine Luckenbach, Georg Schumm, Erhard Seifried, Horst Ritter, Burkhard Bültmann
Journal: New England Journal of Medicine (1996)

Modern concepts of cancer immunology originated from the classic observations by Jensen, Loeb, Tyzzer, and Little in the early years of the 20th century of the rejection of transplanted allogeneic tumors and the acceptance of syngeneic tumors.1 Despite this law of transplantation, there are several clinical examples of the accidental transplantation of a malignant tumor or tumor cells into a healthy recipient.2–5 We describe the accidental transplantation of a malignant sarcoma from a patient to a surgeon. Using molecular methods, we showed that the sarcomas in the unrelated patient and surgeon were genetically identical. Case Report A 32-year-old man . . .
OP0137-PC Decreasing incidence of disability pensions in patients with rheumatoid arthritis in germany:
Authors: D. Pattloch, P. Herzer, S. Zinke, A. Gräßler, A. Zink, A. Strangfeld
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> During the past two decades, the overall incidence of disability pensions (DP) in Germany has decreased tremendously, mainly due to changes in the labour market and in the practice of retirement. In 2010, the rates of new DP in the statutory pension insurance fund were only 52% of those in 1993. When analysing incidence rates of DP among patients with rheumatoid arthritis, these changes in the population have to be taken into account. <h3>Objectives</h3> To compare the incidence of DP in patients enrolled in the German biologics register RABBIT with the expected incidence based upon the general population. <h3>Methods</h3> We used data from 2,385 patients with rheumatoid arthritis (RA) aged 40 to 59 who were enrolled in RABBIT at initiation of a biologic or synthetic DMARD after at least one DMARD failure and did not receive any kind of pension at baseline. Further, we used published data on DP by year, age, and sex from the German statutory pension insurance fund. We applied indirect standardization following the standardized mortality ratio approach and divided the cases observed in RABBIT by the number of cases expected for the age and sex matched population leading to a standardized disability pension ratio (SDR). The observation time was censored by either year of DP, 60th birthday or date of last information on occupational status. <h3>Results</h3> 343 cases with incident DP were observed within 7.437 patient years. The SDR for DP in the RABBIT data compared to the population declined from 10.3 to 3.4 between 2001 and 2010 (Table 1). In the first years of the register, patients who were enrolled at start of a biologic DMARD had a higher risk of DP than those on synthetic DMARDs. This difference disappeared in the last observed years. <h3>Conclusions</h3> Taking the overall population trend into account, the risk of early retirement in patients with RA observed in a large cohort has declined tremendously during the last decade. Patients treated with biologic DMARDs, even though more severely ill at inclusion, now have the same chance of remainig gainfully employed as those with less severe disease under treatment with synthetic DMARDs. This supports the evidence that the prognosis of RA has improved considerably due to better strategies and options of treatment. Supported by a joint unconditional grant from Abbott, Amgen/Swedish Orphan Biovitrum, Bristol Myers Squibb, Essex/MSD, Pfizer, Roche, and UCB. <h3>Disclosure of Interest</h3> None Declared
Abstract 1509: Analysis of interaction between tumor endothelial cells and tumor cells
Authors: Nako Maishi, Noritaka Ohga, Yasuhiro Hida, Yusuke Ohba, Taisuke Kawamoto, Kosuke Akiyama, Kazuko Kitayama, Miyako Kondoh, Takahiro Osawa, Kazuyuki Yamamoto, Nobuo Inoue, Masanobu Shindoh, Kyoko Hida
Journal: Cancer Research (2011)

Abstract Interaction between tumor endothelial cells (TECs) and tumor cells plays a key role in the early stage of hematogenous metastasis. TECs provide the principal route by which tumor cells exit the primary tumor site and enter the circulation. We have reported TECs are different from normal endothelial cells (NECs) in various aspects, such as chromosomal abnormality and gene expression profiles. In this study, we isolated two types of TECs from different human tumor xenografts in nude mice to analyze interaction between TECs and tumor cells. One is HMTEC isolated from highly metastatic tumor and the other is LMTEC from low metastatic tumor. We also isolated NEC from dermis of normal nude mouse as normal control. HMTEC expressed higher levels of mRNA of angiogenesis-related genes than LMTEC or NEC. We hypothesized HMTECs may promote metastasis, in particular, intravasation at the primary site. We investigated the roles of TECs in tumor metastasis; 1) migration of tumor cells towards TECs, 2) adhesion to endothelial layer, 3) crossing the endothelium. Tumor cells migrated towards conditioned-media (CM) from HMTEC more than LMTEC-CM or NEC-CM in vitro. Tumor cells were more adhesive to HMTEC than to LMTEC or NEC. They migrated through the HMTEC-monolayer most among all ECs in transendothelial migration assay. These results suggested that HMTEC may help tumor cells to metastasize. Finally, we analyzed the effects of CM from highly metastatic tumor cells on NECs. Tumor CM induced mRNA expressions of several genes, such as CXCL12, VEGFA and extracellular matrix in NEC. Tumor cells more migrated towards tumor CM- treated NEC than non-treated NEC. Tumor cells adhered more to the tumor CM- treated NEC than to non-treated NEC. These results suggest that TECs “educated” in tumor microenvironment which have highly metastatic potential, may be collaborating with tumor cells for metastasis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1509. doi:10.1158/1538-7445.AM2011-1509
THU0236 Low grade erosive arthritis is a genuine and frequent disease manifestation of systemic sclerosis
Authors: E. Akbayrak, I.H. Tarner, U. Müller-Ladner, R. Dinser
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> The prevalence of arthritis in systemic sclerosis is unclear. Classically, arthritis is attributed to overlapping rheumatoid arthritis. <h3>Objectives</h3> To assess the prevalence of and characterise arthritis in hands of patients with systemic sclerosis, excluding patients with overlapping arthritis forms. <h3>Methods</h3> Low field MRI was performed in consecutive out- and inpatients with systemic sclerosis between January 2010 and March 2011. Results were assessed in a standardised manner summarised in the SCLERART score. <h3>Results</h3> Of 38 inpatients and 8 outpatients, 30 participated. Of 26 patients that could be evaluated, erosions, bone marrow edema, synovitis, and effusions were found in 85%, 36%, 63%, and 56% respectively. 25% of patients had tenovaginitis. Arthritis affected only a few joints per hand analysed. While almost all bones could be affected, synovitis and bone marrow edema occurred most frequently the proximal row of carpal bones, most prominently the os lunatum. The amount of inflammatory changes measured with the SCLEART score correlated significantly with the functional status assessed with the Funktionsfragebogen Hannover FFbH. <h3>Conclusions</h3> Arthritis is a frequent feature in patients with pure systemic sclerosis. The distribution and the MRI characteristics suggest that it may be caused more by ischemic mechanisms than by immunological inflammation. <h3>Disclosure of Interest</h3> None Declared
LECCIONES PRÁCTICAS EN EL MANEJO DE LA PERFORACIÓN GÁSTRICA Y COLÓNICA ASOCIADA A DISECCIÓN SUBMUCOSA ENDOSCÓPICA
Authors: A Herreros De Tejada Echanojauregui, C Cueto Moore, P Matallanos, E Blazquez, JC Fernandez-Diaz, M Gonzalez-Haba, A Sanchez-Movilla, V Rojo, JL Calleja Panero, L Abreu Garcia
Journal: Endoscopy (2013)

Introducción: La disección submucosa endoscópica (DSE) se asocia a un riesgo significativo de perforación. El tratamiento endoscópico mediante cierre del defecto con clips puede evitar la cirugía en un alto porcentaje de casos. Objetivo: Analizar la eficacia y seguridad del tratamiento endoscópico de perforaciones asociadas a DSE gástrica y colorrectal. Material y Métodos: Estudio prospectivo de todos los casos de DSE gástrica o colorrectal realizados en nuestro centro que presentaron como complicación perforación objetivada por el endoscopista y fueron tratados endoscópicamente con endoclips con intención curativa. Se utilizaron de forma única o combinada clips de uso estándar modelos Resolution Clip® (Boston Scientific) y QuickClip® (Olympus). Todos los pacientes se mantuvieron en régimen de ingreso hospitalario al menos 96h tras la DSE, fueron mantenidos en dieta absoluta las primeras 48h y recibieron tratamiento antibiótico durante 10 – 14 días. Resultados: En un periodo de 12 meses (Junio 2012-Junio-2013) se llevaron a cabo 19 DSE (6 colónicas, 6 gástricas, 4 rectales y 2 esofágicas). La tasa de perforación fue del 42% (8 casos). La distribución según localización fue: colon (66% – 4/6;), estómago (50% – 3/6) y recto (25% – 1/4). En la perforación rectal asociada a fracaso técnico se realizó tratamiento quirúrgico electivo por EII (proctocolectomía). En los restantes 7 casos se realizó cierre endoscópico con éxito técnico en el 100%. En el seguimiento posterior (2 – 54 semanas) solo 1 caso (DSE en colon transverso con 4 puntos de perforación) requirió tratamiento quirúrgico (sutura y limpieza) por peritonitis secundaria a las 24h. Los otros 6 casos restantes no presentaron incidencias, con mediana de estancia hospitalaria de 5,5 días (4 – 8). Conclusiones: El riesgo de perforación asociado a DSE es mayor en colon frente a otras localizaciones. El tratamiento endoscópico con clips presenta excelentes resultados y evita un muchos casos la necesidad de abordaje quirúrgico.
Temporary placement of a fully covered self-expanding metal stent to allow therapeutic ERCP
Authors: Benedetto Mangiavillano, Deborah Fisher, Massimo Conio
Journal: Endoscopy (2014)

A 64-year-old man with a previous history of laryngectomy followed by chemo- and radiotherapy was referred to us in January 2014 because of dysphagia. Endoscopy detected a 4-cm-long benign-appearing stricture in the hypopharynx (diameter 7 mm) and a 6-cm-long malignant circumferential lesion in the mid-esophagus (diameter 8 mm).
Case 8-2010
Authors: Robert H. Young, Annekathryn Goodman, Richard T. Penson, Anthony H. Russell, Raul N. Uppot, Rosemary H. Tambouret
Journal: New England Journal of Medicine (2010)

A 22-year-old woman was admitted to this hospital because of hypercalcemia and a pelvic mass. One month before admission, abdominal pain developed, followed by abdominal fullness, anorexia, nausea, vomiting, polydipsia, and nocturia. A computed tomographic scan of the pelvis showed a complex right adnexal mass. The serum calcium level was 17.2 mg per deciliter. A diagnostic procedure was performed.
PM-07 * LOSS OF ATRX DECREASES SURVIVAL AND IMPROVES RESPONSE TO DNA DAMAGING AGENTS IN A NOVEL MOUSE MODEL OF GLIOBLASTOMA
Authors: C. Koschmann, A. Calinescu, D. Thomas, N. Kamran, F. Nunez-Aguilera, M. Dzaman, R. Lemons, Y. Li, H. Roh, P. Lowenstein, M. Castro
Journal: Neuro-Oncology (2014)

Pediatric glioblastoma (GBM) remains one of the most difficult childhood tumors to treat. ATRX is a histone chaperone protein that is mutated primarily in younger patients with GBM. No previous animal model has demonstrated the effect of ATRX loss on GBM formation. We cloned an ATRX knockdown sequence into a Sleeping Beauty (SB) transposase-responsive plasmid (shATRX) for insertion into host genomic DNA. Glioblastomas were induced in mice by injecting plasmids encoding SB transposase/ luciferase, shp53 and NRAS, with or without shATRX, into the ventricle of neonatal mice. Tumors in both groups (with or without shATRX) showed histological hallmarks of human glioblastoma. The loss of ATRX was specifically localized only within tumors generated with the shATRX plasmid and not in the adjacent cortex. Notably, loss of ATRX reduced median survival of mice by 43% (p = 0.012). ATRX-deficient tumors were significantly more likely to develop microsatellite instability (p = 0.014), a hallmark of impaired DNA-damage repair. Analysis of three human GBM sequencing datasets confirmed increased number of somatic nucleotide mutations in ATRX-deficient tumors. Treatment of primary cell cultures generated from mouse GBMs showed that ATRX-deficient tumor cells are significantly more sensitive to DNA damaging agents. In addition, mice with ATRX-deficient GBM treated with whole brain irradiation had trend towards improved survival (p= 0.06), with some long-term survivors. Treated ATRX-deficient tumor cells showed greater evidence of double-stranded DNA breakage, by gH2A.X. In summary, this mouse model prospectively validates ATRX as a tumor suppressor in human GBM for the first time in an animal model. In addition, loss of ATRX leads to increased mutation frequency and response to DNA-damaging therapy. We have generated the hypothesis that ATRX loss leads to a genetically unstable tumor; which is more aggressive when untreated, but more responsive to DNA-damaging therapy, ultimately resulting in equivalent or improved overall survival.
Abstract 3988: Aberrant <i>NFKBIA</i> expression in lung adenocarcinomas arising from never smokers
Authors: Masashi Furukawa, Junichi Soh, Shinichi Toyooka, Kazuhiko Shien, Yuho Maki, Takayuki Muraoka, Norimitsu Tanaka, Tsuyoshi Ueno, Hiroaki Asano, Kazunori Tsukuda, Masaomi Yamane, Takahiro Oto, Shinichiro Miyoshi
Journal: Cancer Research (2012)

Abstract [Background] Lung cancer in never-smoker (NS) is known as a seventh cause of cancer-related death. Activating mutation of epidermal growth factor receptor (EGFR) gene is frequently observed in non-small-cell lung cancers (NSCLCs) with never smoking history and it is significantly associated with the sensitivity of EGFR tyrosine kinase inhibitor (EGFR-TKI). Recently, NFKBIA gene, a tumor suppressor gene, has been reported to be silenced by deletion in glioblastomas, and in NSCLCs, its expression level is associated with clinical outcome of EGFR-TKI therapy. In this study, we examined NFKBIA expression and the genetic alterations of EGFR, K-ras, EML4-ALK to investigate the inter-relationship between these genetic alterations and clinicopathological factors among lung adenocarcinomas in NS. [Material and Method] We obtained ninety seven lung adenocarcinomas samples in NS which were resected at our institution. Four NSCLC cell lines (NCI-H1299, NCI-H1650, HCC827, NCI-H1819) were also investigated. We examined NFKBIA expression using immunohistochemistory (IHC) in tumor samples and real time reverse transcriptional PCR (RT-PCR) in cell lines, respectively. Mutational statuses of EGFR and K-ras genes were determined using mutant-enriched PCR assay and direct sequencing, respectively. EML4-ALK fusion was screened by ALK IHC and confirmed using RT-PCR assay. The methylation status of NFKBIA gene is investigated by methylation specific PCR assay and bisulfite sequencing. [Result] NFKBIA expression was silenced in 36 out of 97 samples (37.1%). EGFR mutation, K-ras mutation, and EML4-ALK fusion were detected in 61.8%, 2.1%, and 1.0% of 97 samples, respectively. Of note, the silencing of NFKBIA expression was significantly frequent in EGFR wild-type patients than in EGFR mutant patients (p=0.0024), while there was no correlation between NFKBIA expression level and K-ras mutation or EML4-ALK fusion. Although NFKBIA expression was silenced in NCI-H1299 and NCI-H1650, NFKBIA expression was not restored after 5-Aza treatment in these cell lines. In addition, NFKBIA methylation was found in these two cell lines. [Conclusion] Our findings suggest that the degradation of NFKBIA expression is a frequent event in lung adenocarcinomas of NS and may mediate an important non-tobacco carcinogenic pathway especially among lung adenocarcinoma with wild-type EGFR. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3988. doi:1538-7445.AM2012-3988
P448Infective endocarditis in patent ductus arteriosus by bartonella sp: case report and literature review
Authors: GBO Guilherme Benfatti Olivato, FRF Fernando Roberto De Fazzio, GAZMVB Marcus Vinicius Burato Gaz, TMS Thaysa Moreira Santos, MRP Milena Ribeiro Paixao, WFG Wilton Francisco Gomes, GBOL Gustavo Benfatti Olivato, DRGD Daniel Renato Goncalves Duarte, TJGT Thais Juliano Garcia Tosta, NMI Nana Miura Ikari
Journal: Cardiovascular Research (2014)

Case report: APS, 19 years, female, outpatients with a diagnosis of patent ductus arteriosus in planning surgical correction sought emergency room presenting episodes of fever measured 2 weeks ago, asthenia and fatigue.Additional research showed pancytopenia and hepatosplenomegaly.Prompted transthoracic echocardiography showing orifice of the ductus arteriosus thicked image hiperrefringente, mobile, looks filamentous pedunculated measuring 13 mm in length, the topography of the ductus arteriosus, pulmonary attached to the end.Requested three pairs of blood cultures without detectable bacterial growth.Patient admitted for investigation and treatment of infective endarteritis probable subacute seronegative blood channel, and also request surveys by indirect immunofluorescence for Bartonella sp, with positive results.Patient admitted for treatment with antibiotics and surgery scheduled for correction of patent ductus arteriosus, and started intravenous penicillin and gentamicin.During admission had septic symptomatic pulmonary embolization, followed by emergency surgery for correction of patent ductus arteriosus.Favorable outcome after high end drug treatment for 28 days.Discussion: Bartonella sp is a common cause of infectious endocarditis seronegative, with few reports in the literature.However, there are no reports in the literature National endarterites ductus arteriosus caused by such bacteria.Since this diagnosis is considered difficult at the beginning of the investigation, it should search request Bartonella sp in all cases of infective endocarditis and endarteritis in patent ductus ateriosus with negative blood cultures, since the prevalence of this microorganism has been increasing in Brazil.
P4-09-16: Factors Affecting the Development of Axillary Lymph Node Metastases in T1a-T1b Breast Cancers.
Authors: TA Khair, SK Boolbol, K Boachi-Adjei, P Klein
Journal: Cancer Research (2011)

Abstract Background: The presence of axillary nodal metastases is a poor prognostic indicator in patients with breast cancer. In the majority of patients, axillary nodal involvement correlates with tumor size and most commonly in tumors greater that 1 cm. Many series report an incidence of axillary nodal metastases in 3–37% of patients with tumors less than or equal to 2 cm. The presence of axillary nodal metastases will often change the stage of the cancer and role of adjuvant systemic treatment; therefore, it is important to understand what features are associated with these small lymph node positive tumors. The purpose of this study is to identify characteristics of T1a and T1b breast cancers that present with axillary nodal metastasis. Methods: A retrospective review of a prospective database identified 878 patients with T1a and T1b breast cancer. Pathologic features including tumor grade, hormone receptor status, lymphatic and vascular invasion, Her-2 neu status, and tumor histology were examined in patients with and without axillary nodal metastases. Pearson's χ2 test along with stepwise linear regression analysis were used to determine which factors were associated with the development of axillary nodal metastases. Results: There were 289 T1a lesions and 589 T1b lesions studied. Four independent variables were found to be associated with axillary nodal metastases in T1a-T1b breast cancer. As illustrated in Table 1, a significant difference was seen in nodal positivity among patients with ER negative tumors compared to those with ER positive tumors (p=.0000). An increased incidence of nodal positivity was also observed among patients with LVI compared with those without LVI (p= .0000). A significant difference was observed in nodal positivity among patients with perineural invasion compared to those without (p=.0006). An increased incidence of nodal positivity was also observed among T1a and T1b tumors with a higher histologic grade (p= 0409, p= .0001). Of note, when comparing T1a to T1b breast cancers, there was no significant difference found among the identified exposure variables of interest. Conclusion: In this series, the overall prevalence of nodal positivity in T1a and T1b breast cancer is 12%. An absence of a relationship between T1a and T1b cancers with nodal positivity was observed. However, the presence of nodal positivity showed a significant correlation with ER negativity, LVI, perineural invasion, and higher histologic grades. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P4-09-16.
A Simple Nonradioisotope Technic for the Determination of Platelet Life-Span
Authors: Marie J. Stuart, Scott Murphy, Frank A. Oski
Journal: New England Journal of Medicine (1975)

Acetylsalicylic acid was shown both in vivo and in vitro to prevent the platelet lipid peroxidation normally induced by the aggregating agents thrombin and epinephrine, and the sulfhydryl inhibitor N-ethylmaleimide. After aspirin ingestion, there was a significant reduction (p smaller than 0.005) in platelet lipid peroxidation, with a gradual return to base-line values over a 10-day period. With these values, a normal platelet survival curve was constructed with a mean half-life of 4.4 days (range of 2.9 to 5.9 days). These values agree with the standard 51-Cr survivals in three patients with chronic idiopathic thrombocytopenic purpura. Half-lives of 1.0, 2.5, and 4.1 days by lipid peroxide technic compared with 1.9, 2.5, and 3.9 days by the concurrent use of 51-Cr. Thus, the technic may be used to measure platelet survival.
Pretreatment Serum Albumin Level is an Independent Prognostic Biomarker in Malignant Pleural Mesothelioma
Authors: T. Klikovits, P. Stockhammer, Y. Dong, M. Jakopovic, L. Brcic, B. Dome, B. Hegedus, M. Samarzija, W. Klepetko, M.A. Hoda
Journal: Annals of Oncology (2015)