tbuckley/cabot-search huggingface dataset:
Characterization of Binding Proteins from Ovarian Carcinoma and Kidney Tubule Cells that are Specific for Cisplatin Modified DNA
Authors: P A Andrews, J A Jones
Journal: Cancer Communications (1991)

We have detected proteins in nuclear extracts from ovarian carcinoma cells and kidney tubule cells that bind specifically to platinated DNA. A 123-bp restriction fragment was platinated with cisplatin (DDP) to a formal molar platinum to nucleotide ratio of 0.05 and end-labeled with [32P]-dCTP. Incubation with nuclear extracts from 2008 human ovarian carcinoma cells caused shifts in the mobility of this probe in non-denaturing polyacrylamide gels. Proteinase K, but not ribonuclease A, destroyed the bands. Comparison of the shifted bands generated by DDP-resistant 2008 and A2780 human ovarian carcinoma cell nuclear extracts with bands from the corresponding sensitive cells showed no differences in protein levels. The affinity of the proteins for the probe was the same in sensitive and resistant 2008 nuclear extracts as determined by competition with platinated salmon sperm DNA. These proteins also bound to a probe damaged with 1,2-diaminocyclohexaneplatinum(II) dichloride but did not bind to a trans-DDP platinated probe. No differences were found in the levels of UV4 or UV5 Chinese hamster ovary cells, which were hypersensitive to DDP compared to wild-type AA8 cells. MDCK and LLC-PK1 kidney tubule cells, which were more resistant to DDP cytotoxicity than 2008 cells, exhibited decreased levels of these proteins. We conclude that, although these proteins that recognize DDP damage in DNA may be involved in excision repair, their levels did not correlate with DDP sensitivity in this panel of cell lines.
Bevacizumab/erlotinib (BEER) as first-line treatment for untreated, advanced non-squamous non-small cell lung cancer (NSNSCLC).
Authors: W. L. Akerley, N. T. Rich, L. Egbert, W. G. Harker, T. Van Duren, J. Smit, J. M. Hoffman
Journal: Journal of Clinical Oncology (2010)

e18008 Background: Bevacizumab and erlotinib improve survival in Advanced NSCLC. Veristrat assay predicts survival of those treated with EGFR inhibitors. Methods: Eligibility criteria included stage IIIB/IV or recurrent, Non-squamous NSCLC, no prior chemotherapy for systemic disease, PS = 0-1, and no hemoptysis. Patients received bevacizumab 15 mg/kg q 3 wks and erlotinib 150 mg po/day until objective or subjective progression. Serum samples were banked. Results: 40 patients have been accrued. The median age was 65 (45-89), 29 had PS = 1, 11 were never-smokers, and 25 were former smokers. Histologies were: adeno 29, BAC 2, NOS 9. The major toxicities were rash (Gr1 = 6, Gr2 = 14, Gr3 = 11) and diarrhea (Gr 1 = 6, Gr2 = 4, Gr 3 = 12). 39 are evaluable for best response, which identified 9 (23%) with PR, 22 with stable and 11 with progression. The median time on erlotinib was 15.1 wks. The median survival was 50.4 wks with 19 pts still alive. Of 32 who have come off therapy, there were 19 with objective progression (OP), 11 with subjective progression (SP) and 2 with intolerance (RPLS, keratoconjunctivitis). There was a trend for worse survival for SP (24 wks) v OP (56 wks). 12 progressors never received subsequent chemotherapy. Veristrat assays were performed in 30. The median PFS for those with good risk was 16.5 v 9.3 wks (p < 0.001) and survival was 79.1 v 12.5 wks (p < 0.001). Conclusions: Bevacizumab and erlotinib are active in unselected, untreated NSNSCLC. The Veristrat assay is an effective predictor of benefit and SP warrants further study as it may be more important than OP (implication for maintenance trials). Author Disclosure Employment or Leadership Position Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Genentech, Huntsman Cancer Institute at the University of Utah
Targeting N-Terminal Pro-Brain Natriuretic Peptide in Older Versus Younger Acute Decompensated Heart Failure Patients
Authors: Susan Stienen, Khibar Salah, Luc W Eurlings, Paulo Bettencourt, Joana M Pimenta, Marco Metra, Antoni Bayes-Genis, Valerio Verdiani, Luca Bettari, Valentina Lazzarini, Jan P Tijssen, Yigal M Pinto, Wouter E Kok
Journal: JACC Heart Failure (2016)
Case 5-1986
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, Betty U. McNeely, L. Penfield Faber, Eugene J. Mark
Journal: New England Journal of Medicine (1986)

Presentation of CaseA 64-year-old woman was admitted to the hospital because of a left hilar mass.She was well until 15 years earlier, when a routine x-ray film of the chest was reported to show a left hilar mass that was believed to represent enlarged hilar lymph nodes. During the succeeding 15 years the patient was seen by several physicians; repeated x-ray films of the chest revealed gradual enlargement of the mass (Fig. 1). Five years before admission a persistent nonproductive cough developed and was most prominent when the patient was recumbent, subsiding as she fell asleep. A few . . .
Stem cell transplantation for tetratricopeptide repeat domain 7A deficiency: long-term follow-up
Authors: Jochen Kammermeier, Giovanna Lucchini, Sung-Yun Pai, Austen Worth, Dyanne Rampling, Persis Amrolia, Juliana Silva, Robert Chiesa, Kanchan Rao, Gabriele Noble-Jamieson, Marco Gasparetto, Drew Ellershaw, Holm Uhlig, Neil Sebire, Mamoun Elawad, Luigi Notarangelo, Neil Shah, Paul Veys
Journal: Blood (2016)
Comparing Use of Low-Value Health Care Services Among U.S. Advanced Practice Clinicians and Physicians
Authors: John N. Mafi, Christina C. Wee, Roger B. Davis, Bruce E. Landon
Journal: Annals of Internal Medicine (2016)

Many physicians believe that advanced practice clinicians (APCs [nurse practitioners and physician assistants]) provide care of relatively lower value.To compare use of low-value services among U.S. APCs and physicians.Service use after primary care visits was evaluated for 3 conditions after adjustment for patient and provider characteristics and year. Patients with guideline-based red flags were excluded and analyses stratified by office- versus hospital-based visits, acute versus nonacute presentations, and whether clinicians self-identified as the patient's primary care provider (PCP).National Ambulatory Medical Care Survey (NAMCS) and National Hospital Ambulatory Medical Care Survey (NHAMCS), 1997 to 2011.Patients presenting with upper respiratory infections (URIs), back pain, or headache.Use of guideline-discordant antibiotics (for URIs), radiography (for URIs and back pain), computed tomography (CT) or magnetic resonance imaging (MRI) (for headache and back pain), and referrals to other physicians (for all 3 conditions).12 170 physician and 473 APC office-based visits and 13 359 physician and 2947 APC hospital-based visits were identified. Although office-based clinicians saw similar patients, hospital-based APCs saw younger patients (mean age, 42.6 vs. 45.0 years; P < 0.001), and practiced in urban settings less frequently (49.7% vs. 81.7% of visits; P < 0.001) than hospital-based physicians. Unadjusted and adjusted results revealed that APCs ordered antibiotics, CT or MRI, radiography, and referrals as often as physicians in both settings. Stratification suggested that self-identified PCP APCs ordered more services than PCP physicians in the hospital-based setting.NHAMCS reflects hospital-based APC care; NAMCS samples physician practices and likely underrepresents office-based APCs.APCs and physicians provided an equivalent amount of low-value health services, dispelling physicians' perceptions that APCs provide lower-value care than physicians for these common conditions.U.S. Health Services and Research Administration, Ryoichi Sasakawa Fellowship Fund, and National Institutes of Health.
PTH-083 Selective monitoring of antitnf drug levels and antibodies will reduce short-term prescribing costs and provide early personalised tailoring of antitnf therapy in routine ibd practice: Abstract PTH-083 Table 1
Authors: TA Tandiari, A Ibarra, L Younge, A Palma Allepuz, A Thompson, U Meade, Z Arkir, E Giles, L Langmead, JO Lindsay, G Parkes, D Rampton
Journal: Gut (2015)

<h3>Introduction</h3> Clinical trial results suggest that measurement of antiTNF drug levels (ATL) and antibodies (ATA) may have a cost-saving as well as clinically beneficial effect in patients with IBD. We aimed to assess whether selective monitoring of ATL and ATA reduces prescribing costs and how it affects clinical decisions in ordinary clinical practice. <h3>Method</h3> ATL and ATA were assayed in 47 (19%) of our 247 antiTNF-treated IBD patients (Crohn’s disease 41, ulcerative colitis 4 and pouchitis 2; (on infliximab 45, on adalimumab 2)) because of either secondary loss of response (n = 11) or a dosage of infliximab (IFX) exceeding 5 mg/kg every 8 weeks (n = 36). ATL and ATA were measured by automated LISA-TRACKER assay (Theradiag, France) at Viapath, St Thomas’ Hospital. For each patient, the outcomes of decisions about therapeutic strategy based on clinical assessment alone were compared against those based on assay results combined with clinical criteria. AntiTNF prescribing and surgery costs were predicted for the ensuing 4 months for the two decision-making strategies, assuming that infliximab phials were shared. <h3>Results</h3> Outcomes of the decisions taken are summarised in the Table. Prescribing costs estimated for the subsequent 4 months (17 weeks) for clinically based decisions were £225,825 and for assay based strategies were £209,772 (£3525 assay costs included). Predicted total cost savings using the assay based decisions, taking into account referrals for surgery and for trial drugs, were £16,053 in 4 months. Clinically based decisions led to more patients staying on antiTNFs, while assay-based decisions caused more patients to be switched to an alternative antiTNF, recruited to trials and referred for surgery. In 19/47 patients, both decision-making approaches gave the same outcome. <h3>Conclusion</h3> Measurement of antiTNF drug levels and antibodies in selected patients should produce short-term cost savings in ordinary clinical practice. Longer follow-up is needed to assess the clinical impact of the decisions made using these assays in combination with clinical criteria, but is likely to amplify the savings they make possible. <h3>Disclosure of interest</h3> None Declared.
SURGICAL MANAGEMENT OF LUNG CANCER
Authors: W E Morgan
Journal: JAMA (1962)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
IL-22 Is an Intestinal Stem Cell Growth Factor, and IL-22 Administration in Vivo Reduces Morbidity and Mortality in Murine GvHD
Authors: Caroline A Lindemans, Anna Mertelsmann, Margaret Helen O'Connor, Jarrod A Dudakov, Robert Jenq, Enrico Velardi, Lauren F. Young, Odette M Smith, Gillian Lawrence, Natalie Luo, Juliet Ivanov, G Hua, Maria L Martin, Chen Liu, Richard Kolesnick, Marcel Van Den Brink, Alan M Hanash
Journal: Blood (2014)
Kaposi's Sarcoma, Vascular Permeability, and Scientific Integrity-Reply
Authors: Marlys H. Witte
Journal: JAMA (1994)

In Reply.  —Our JAMA article, in keeping with our original correspondence with Science on April 24,1992, July 30,1992, and September 3, 1992, sought clarification of three specific issues related to the enhanced vascular permeability reported in 12-hour KS  in the Science article by Dr Nakamura and colleagues 1 : (1) intense diffuse EB staining of tails in untreated but not SP-PG—treated nude mice; (2) easy extraction of extravasated tissue-bound EB-albumin complex using overnight incubation in PBS at 4°C; and (3) extraordinarily rapid extravasation of EB-albumin complex into tiny 12-hour KS lesions within 15 minutes of tail vein EB injection.  Whereas heightened vascular leakiness, in addition to well-documented lymphatic dysplasia and obstruction, 2 may contribute to edema in AIDS-associated KS and angioinhibitory agents theoretically lessen such edema, the report by Nakamura et al in Science , a letter sent by Dr Gallo to Science on June 9,1992, and Science 's two tiers
Treatment of Ex Vivo Expanded NK Cells with Daratumumab F(ab')2 Fragments Protects Adoptively Transferred NK Cells from Daratumumab-Mediated Killing and Augments Daratumumab-Induced Antibody Dependent Cellular Toxicity (ADCC) of Myeloma
Authors: Elena Cherkasova, Luis Espinoza, Ritesh Kotecha, Robert N. Reger, Maria Berg, Georg Aue, Ricardo M. Attar, A Kate Sasser, Mattias Carlsten, Richard W. Childs
Journal: Blood (2015)
The Adverse Influence of Pretransplant Interferon- (IFN-) on Transplant Outcome After Marrow Transplantation for Chronic Phase Chronic Myelogenous Leukemia Increases With the Duration of IFN- Exposure
Authors: Dietrich W. Beelen, Ahmet H. Elmaagacli, Prof. Ulrich W. Schaefer
Journal: Blood (1999)
Outbreak of measles in two private religious schools in Bourgogne and Nord-Pas-de-Calais regions of France, May-July 2008 (preliminary results)
Authors: U Noury, J Stoll, S Haeghebaert, D Antona, I Parent du Châtelet, Collective The investigation team
Journal: Eurosurveillance (2008)

To date, 110 cases of measles have been identified by local health authorities in the Bourgogne and Nord-Pas-de-Calais regions of France, with onset of symptoms between 3 May and 19 July. The first three cases were reported on 25 June by a general practitioner to the French national institute of health (Institut de Veille Sanitaire, InVS) regional office in Bourgogne, in the centre of France. The three unvaccinated cases were students at a private religious school for girls located in Bourgogne and had onset of rash between mid-May and 23 June. On the same day, another general practitioner in Nord-Pas-de-Calais reported a case of measles in an unvaccinated 14 year-old boy attending a private religious boarding school for boys. The boy had developed a rash on 19 June and happened to live in the same place in Bourgogne where the above-mentioned girls' school was located. The two schools have elementary to secondary students. Both are private religious boarding schools. Most of the students come from the surrounding areas, some resident pupils are from other French regions, and some from abroad. All students returned home on 26 June for summer holidays.
HIV and tuberculosis in prisons in sub-Saharan Africa
Authors: Lilanganee Telisinghe, Salome Charalambous, Stephanie M Topp, Michael E Herce, Christopher J Hoffmann, Peter Barron, Erik J Schouten, Andreas Jahn, Rony Zachariah, Anthony D Harries, Chris Beyrer, Joseph J Amon
Journal: The Lancet (2016)
Patients' rights: a cultural challenge to Western psychiatry
Authors: E B Brody
Journal: American Journal of Psychiatry (1985)

To some degree, all psychiatric encounters, by constraining behavioral variation and autonomous decision making, threaten a personhood already impaired by illness. The challenge presented by this and other factors in Western psychiatry is how to resolve the ethical dilemmas of justice, autonomy, and beneficence inherent in contemporary hospitalization and treatment practices for severely mentally ill persons. The author discusses this challenge in terms of the physician-patient contract, clinical standards for limiting autonomy, and the Kantian concept of equality of mutual respect. The legal code in relation to psychiatry has evolved into a contemporary interpreting mechanism for Kantian cultural tradition.
Doxorubicin-Induced Myocardial Injury
Authors: George Fujisaki, Chiho Inokuchi, Naoko Murashige
Journal: New England Journal of Medicine (2004)

the editorialist replies: Drs. Primack and Estes cite wholesale costs for 5 percent albumin and for normal saline, suggesting that intensivists should be aware of this difference. Although few may be able to cite institution-specific prices, I hope that all intensivists are familiar with this cost differential. An economic analysis requires quantitatively comparing two or more interventions with respect to both resource use and clinical end points. Accordingly, some intensivists will point to equivalent outcomes in the SAFE Study, including the lack of harm overall, and use albumin selectively based on a pathophysiological rationale. As I stated in my editorial, “Others will conclude that without proof of benefit, routine use of albumin is hard to justify; for similar clinical outcomes at a lower cost, crystalloids may suffice in most circumstances.” Primack and Estes highlight the fact that the costs of albumin may not be reimbursed by insurers. In countries with universal health care, insurance policies do not influence fluid choices, although rationing may occur for interventions without promise of a health advantage. As I noted, the rate of use of albumin will thus reflect interpretation of the SAFE Study’s point estimate and confidence limits, as well as “patient-specific conditions, clinicians’ preferences, perceptions regarding the safety of biologic fluids, availability, and cost.”
Liver-infiltrating CD11b−CD27− NK subsets account for NK-cell dysfunction in patients with hepatocellular carcinoma and are associated with tumor progression
Authors: Qiong-Fang Zhang, Wen-Wei Yin, Yang Xia, Ya-Yang Yi, Qiu-Feng He, Xing Wang, Hong Ren, Da-Zhi Zhang
Journal: Cellular and Molecular Immunology (2016)

Natural killer (NK) cells have a vital role in killing hepatocellular carcinoma (HCC) cells; however, the mechanism underlying tumor-infiltrating NK (TINK)-cell dysfunction remains poorly understood. Using flow cytometry staining, we precisely characterized the frequency, phenotype and function of NK subsets distinguished by CD27 and CD11b in 30 patients with HCC in comparison to 30 healthy controls. Interestingly, we found a substantial proportion of liver-infiltrating CD11b−CD27− (DN) NK subsets in tumor tissue from HCC patients. Remarkably, these relatively expanded DN NK subsets exhibited an inactive and immature phenotype. By detecting the expression of CD107a and interferon-gamma (IFN-γ) on NK subsets and NK cells, we demonstrated that DN NK subsets exhibited a poor cytotoxic capacity and deficient potential to produce IFN-γ in comparison to the other three subsets, which contributed to the dysfunction of TINK cells in HCC patients. In addition, we found that the presence of DN NK cells was closely associated with the clinical outcomes of HCC patients, as the frequency of DN NK cells among TINK cells was positively correlated with tumor stage and size. A large percentage of DN NK cells among TINK cells was an independent prognostic factor for lower survival in the 60-month follow-up period. In conclusion, a substantial proportion of CD11b−CD27−NK subsets among TINK cells accounts for NK-cell dysfunction in patients with HCC and is associated with tumor progression. Our study may provide a novel therapeutic target for the treatment of patients with HCC.
Atypical facial pain as a defense against psychosis
Authors: J F Delaney
Journal: American Journal of Psychiatry (1976)

The author describes three women who presented psychotic symptoms 24--48 hours before scheduled neurosurgical procedures for atypical facial pain; all had had extensive dental reconstruction and attempted nerve blocks with no relief. Psychiatric hospitalization and administration of major tranquilizers resulted in control of symptoms and relief of pain. Two patients were followed for a year and have had return of psychiatric symptoms or facial pain; both have been maintained on medication and have returned to normal activities. The author suggests that the facial pain may have served as a defense against the emergence of psychosis.
EPIC Early Detection of Ovarian Cancer
Authors: Steven J. Skates
Journal: Clinical Cancer Research (2016)

CA125 dominated performance for ovarian cancer early detection among four serum biomarkers evaluated in EPIC study prediagnostic serum, rising on average 3 years prior to detection. Adding HE4 provided only marginal improvement. This natural history supports annual testing for early detection and highlights the importance of biomarker discovery complementing CA125. Clin Cancer Res; 22(18); 4542-4. ©2016 AACRSee related article by Terry et al., p. 4664.
Case 12-1970
Authors: Richard C. Cabot, Benjamin Castleman, Betty U. McNeely
Journal: New England Journal of Medicine (1970)

Presentation of Case First admission. A forty-three-year-old man was admitted to the hospital for evaluation of pneumonia. He had been well until nine months previously, when fever and a cough developed. X-ray films of the chest (Fig. 1) showed an abscess in the lower lobe of the right lung. Antibiotics were administered, and x-ray films of the chest taken four months later revealed almost complete clearing. He returned to work and was well until three weeks before entry, when he had a cold accompanied by a cough and weakness. An x-ray film of the chest showed a large infiltrate . . .
ATPS-51ESTABLISHING THE ROLE OF ARGININE DEPRIVATION WITH THE RECOMBINANT HUMAN ARGINASE PEG-BCT-100 IN THE TREATMENT OF ADULT GLIOMAS
Authors: Herbert Loong, C.T. Choy, H.T. Cheong, Y. Li, K.P. U, Aden Chan, C.H. Wong
Journal: Neuro-Oncology (2015)

BACKGROUND: Arginine deprivation is a novel approach to limit arginine-dependent tumour growth. The presence of enzymes involved in the de novo synthesis of arginine from citrulline, argininosuccinate synthetase (ASS), argininosuccinate lyase (ASL) and ornithine transcarbamylase (OTC), can influence the sensitivity of tumour to arginine depletion. ASS1, ASL and OTC expressions have been previously described in pediatric gliomas, but such data is lacking in adults. We studied the efficacy of PEG-BCT-100 (also known as rhArg1peg5000), a PEGylated recombinant human arginase 1 on glioma cell lines. METHODS: Cells from 5 representative glioma cell lines (A172, M059J, M059K, T98G and U-87 MG) either incubated in full culture medium (control), arginine free medium or PEG-BCT-100 (1U/ml) treated medium. They were monitored by live cell imaging for 72 hours. Their corresponding IC50 were determined by cell viability assay. An analysis of ASS1, ASL and OTC expression in adult gliomas determined from archival samples. RESULTS: All cell lines were sensitive to PEG-BCT-100 and demonstrated significant cell proliferation inhibition. Their IC50, as determined by cell viability assay were 0.156 U/ml, 0.300 U/ml, 0.312 U/ml, 0.415 U/ml, and 0.522 U/ml for T98G, M059J, A172, U-87 MG and M059K respectively. In particular, cell death with both apoptosis and necrosis were observed in T98G, M059K and A172 cells. When cultured in arginine free medium, growth inhibition were only observed in M059J, M059K and T98G cells, indicating that these cells may be more auxotrophic to arginine. The determination of basal ASS1, ASL an OTC expression in 50 archival samples of adult gliomas is on-going. CONCLUSIONS: Arginine deprivation by PEG-BCT-100 is effective in suppressing glioma cell growth in vitro, suggesting arginine auxotrophism in gliomas. Identification of OTC, ASS and ASL expression levels in adult gliomas may potentially establish a role for these proteins as a predictive biomarker of response to arginine-deprivation therapy.