tbuckley/cabot-search huggingface dataset:
AB0311 Evaluating of Atherosclerosis in Rheumatoid Arthritis Patients with Carotid Intima MedİA ThİCkness and Lipoprotein Phospholipase A2 Activity
Authors: C. Bes, S. Gürel, G. Buğdaycı, O. Dikbaş, M. Soy
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Rheumatoid arthritis is associated with increased morbidity and mortality due to atherosclerotic cardiovascular diseases. Lipoprotein associated phospholipase A2 (Lp-PLA2) is an enzyme of phospholipase A2, it plays important role in inflammation and atherosclerosis. Here in we aimed to evaluate the Lp-PLA2 activity in rheumatoid arthritis patients and compare the Lp-PLA2 activity with carotid intima media thickness (CIMT). <h3>Objectives</h3> In this study we aimed to evaluate the Lp-PLA2 activity in rheumatoid arthritis patients and compare the Lp-PLA2 activity with carotid intima media thickness (CIMT). <h3>Methods</h3> 25 consecutive patients with rheumatoid arthritis and forty controls (20 patients with type 2 diabetes mellitus (DM) and 20 healthy controls) were included in the study. The fasting blood samples were tested for Lp-PLA2, complete blood count, glucose, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), serum high density lipoprotein cholesterol (HDL-cholesterol), LDL-cholesterol, and total cholesterol. Frozen serum samples were used for analyzing Lp-PLA2. Disease activity was calculated with DAS-28; HAQ score was evaluated in rheumatoid arthritis group. The mean CIMT was calculated in all participants. <h3>Results</h3> Lp-PLA2 activity was significantly higher in DM group (P: 0,006) and LDL-cholesterol levels were lower in rheumatoid arthritis and healthy control groups compared with diabetics (p: 0,001 and p: 0,029, respectively). The mean CIMT were significantly higher in patients with type 2 DM (p: 0,047). Lp-PLA2 activity was not increased in rheumatoid arthritis group when compared with healthy controls. However, Lp-PLA2 activity was significantly higher in the diabetic patients than those in the RA group and in the healthy controls. The mean CIMT were not distinct in rheumatoid arthritis group when compared with healthy controls. This result may be associated with low disease activity scores in the patients with rheumatoid arthritis. <h3>Conclusions</h3> As a result an Lp-PLA2 activity in RA patients was similar to healthy control group but lower than diabetics. No correlation was determined between CIMT and Lp-PLA2 activity, which may be associated with our RA patients were inactive and under therapy. LpPLA2 activity and CIMT measurement may give more knowledge on large population of RA patients with active disease. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5371
Genome sequences of segmented filamentous bacteria in animals
Authors: Michele Caselli, Francesca Cassol, Valentina Gentili, Dario Di Luca
Journal: Gut Microbes (2012)

Experimental studies indicate that segmented filamentous bacteria (SFB) can recapitulate fundamental immune responses, particularly in reference to Th 17 and regulatory T-cell activity. The recent description of whole genomic DNA sequences of mouse and rat SFBs and the comparison between these sequences opens new important perspectives. In particular this knowledge allows to perform quali-quantitative studies in human beings regarding these yet unculturable anaerobic bacteria. The comparative analysis of rat and mouse SFB 16S rRNA sequences suggests the existence of highly conserved regions that could represent SFB-specific molecular targets potentially useful to develop PCR probes for study SFB in humans. These molecular tools may be of inestimable value to evaluate the possible role played by SFB in the immune system physiology, as well as its potential involvement in human chronic inflammatory and autoimmune diseases.
P166 Effects of low-vs high-dose fluticasone/formoterol combination therapy on AMP challenge in asthmatic patients
Authors: F Kanniess, Z Diamant, M Lomax, M Jain
Journal: Thorax (2013)

<h3>Background</h3> The ICS fluticasone propionate (FP) and the LABA formoterol fumarate (FORM) have now been combined in a single aerosol inhaler (FP/FORM; <b><i>flutiform</i></b><sup>®</sup>). The effect of low- (2 puffs 50/5 µg bid) vs high-dose (2 puffs 250/10 µg bid) FP/FORM on airway responsiveness to AMP was compared in an incomplete block, placebo-controlled, 2-way crossover study. <i>Post hoc </i>data analysis from patients who received both FP/FORM doses is presented. <h3>Methods</h3> 62 patients (33M, 29F; =18yrs; reversible FEV<sub>1</sub> =60% pred.) discontinued maintenance ICS medication for 2 - 3wks; those showing a provocative dose of AMP producing a 20% decline in FEV<sub>1</sub> (AMP PD<sub>20</sub> FEV<sub>1</sub>) of &lt;60 mg were randomised to receive 2 of 3 treatments (FP/FORM high-, low-dose or placebo) during 2 periods of 28 ± 3 days each, separated by 2 - 3wks. AMP challenges were performed pre-dose and repeated 12h after last dose at the end of each treatment period. The difference in changes in AMP PD<sub>20</sub> FEV<sub>1</sub> (day 1 vs day 28) between treatments were compared by an ANCOVA. <h3>Results</h3> 15 patients were randomised to receive both high- and low-dose FP/FORM. The change in AMP PD<sub>20 </sub>FEV<sub>1</sub> was greater with FP/FORM high- compared with low-dose (LS means: high dose = 11 mg; 95% CI 4.3, 27.9; low dose = 4.6 mg, 95% CI 1.8, 11.8), with a statistically significant 2.4 fold difference in AMP PD<sub>20</sub> FEV<sub>1</sub> (1.2 doubling doses) between doses (LS mean: 2.4; 95% CI 1.3, 4.5; p = 0.012). FP/FORM was well-tolerated; only few (mild or moderate) AEs occurred. <h3>Conclusions</h3> A significant dose-response was found between low- and high-dose FP/FORM with the higher dose demonstrating a greater reduction in airway responsiveness to AMP.
P1189 HIGH MIG (CXCL9) PLASMA LEVEL FAVORS RESPONSE TO PEGINTERFERON AND RIBAVIRIN IN HCV INFECTED PATIENTS BUT IS NOT LINKED TO DPP4 ACTIVITY
Authors: S. Johansson, W. Talloen, M. Tuefferd, J. Darling, G. Fanning, M.W. Fried, J. Aerssens
Journal: Journal of Hepatology (2014)
SAT0421 Subclinical limited joint mobility in diabetic patients: A marker of micro-vascular complications
Authors: A.J. Mathew, K. Lavanya, M. Hariharan, D.J. Fernando
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Objectives</h3> To study the association of subclinical limited joint mobility (SLJM) with micro-vascular complications of type 2 diabetes mellitus (T2DM). <h3>Methods</h3> T2DM patients from a tertiary care diabetic centre were compared with an equal number of age- and sex-matched non-diabetic controls from the community. Flexion and extension at the MCP and PIP joints of the non-dominant hand were measured in both groups using a validated finger goniometer. SLJM was defined as 2SD below the flexion and extension at MCP in the control group. Peripheral neuropathy (PN) was assessed using a standardized and validated screening test. Nephropathy (DN) was assessed using standardized dipsticks for microalbuminuria. Retinopathy (DR) was diagnosed and scored using a direct ophthalmoscope. <h3>Results</h3> 236 cases and controls (Mean age: 50.7±12.2 years; 53.4% males) were analysed. The prevalence of cheiroarthropathy among the cases was 23.7%. SLJM (flexion) at the MCP and PIP joints were 42.8% and 50.3%, respectively and SLJM (extension) at the MCP was 37.3%. Table 1 gives the association of SLJM with variables. In regression analysis, PN and DN were the independent risk factors for SLJM. <h3>Conclusions</h3> Goniometry may be proposed as a non-invasive and easily reproducible test for screening micro-vascular complications of T2DM in the community. <h3>Disclosure of Interest</h3> None Declared
Recombinant Adeno-associated Virus Serotypes 2– and 5–Mediated Gene Transfer in the Mammalian Brain: Quantitative Analysis of Heparin Co-infusion
Authors: Mihail Y. Mastakov, Kristin Baer, Robert M. Kotin, Matthew J. During
Journal: Molecular Therapy (2002)

Recombinant adeno-associated viruses (rAAVs) are among the most promising vectors for gene delivery into the CNS. However, a major hurdle for gene transfer to the mammalian brain is to achieve high transduction levels in target cells beyond the immediate injection site. Therefore, building upon the optimization of injection parameters on which we have recently reported, it is important to define additional methods to increase the volume of distribution. Here, we establish an optimal heparin concentration, and show that co-injection of heparin together with rAAV2 leads to a significantly higher and more homogeneous distribution of transduced cells. In contrast, the diffusion pattern of rAAV serotype 5 differs from that of rAAV2, in that its distribution is less homogeneous, more variable, and patchy. Furthermore, this study illustrates the influence of receptor binding on the expression pattern following injection of rAAV in the CNS. In addition to improvements in expression cassettes and viral titers and the use of very slow infusion rates, gene transfer studies in the CNS where the goal is to obtain widespread transduction should consider co-injecting the viral vector rAAV2 with heparin to maximize transduction efficiency and viral spread.
A2.27 The role of SYK in contact hypersensitivity
Authors: JZ Csepregi, T Németh, FC Weber, SF Martin, A Mócsai
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background and objectives</h3> Allergic contact dermatitis (ACD) is a common inflammatory skin disease, triggered by repeated exposure to contact allergens. ACD and its animal model, contact hypersensitivity (CHS), is a T-cell mediated delayed-type hypersensitivity reaction, which can be divided in sensitisation and elicitation phases. The Syk tyrosine plays important roles in several cell types known to be involved in the CHS response. Therefore, we tested whether Syk plays a role in CHS development, with particular emphasis on its importance in myeloid cells and its role in the sensitisation phase. <h3>Materials and methods</h3> Bone marrow chimaeras with Syk<sup>–/–</sup> or μMT hematopoietic system were generated by fetal liver or bone marrow transplantation, respectively. Myeloid- and neutrophil-specific Syk deletion was achieved by crossing LysM-Cre and MRP8-Cre mice with Syk<sup>flox/flox</sup> animals (LysM-Cre Syk<sup>f/f</sup> and MRP8-Cre Syk<sup>f/f</sup>). CHS was triggered by sensitisation with the contact allergen TNCB, followed by elicitation with TNCB on the ears 5 days later. The role of Syk in the sensitisation phase was tested by <i>in vitro</i> T-cell re-stimulation with TNBS using IFNγ production as a functional readout. <h3>Results</h3> While repeated exposure to TNCB triggered a robust increase in ear thickness in wild type mice or chimaeras, no such increase could be observed in Syk<sup>–/–</sup>chimaeras, indicating an important role for Syk in the CHS response. As Syk<sup>–/–</sup>chimaeras have no B-cells, we tested the possible contribution of these cells using a B-cell deficient mouse strain (μMT). There was no difference between the CHS response of wild type and B-cell deficient chimaeras, arguing against the role of B-cells in CHS development. Lineage-specific deletion studies revealed significant reduction in the ear thickness increase in LysM-Cre Syk<sup>f/f</sup> and MRP8-Cre Syk<sup>f/f</sup>mice, indicating an important role for Syk expression within the myeloid, and in particular within the neutrophil compartment. Syk deficiency significantly reduced the <i>in vitro</i> release of IFNγ by TNBS re-stimulated T-cells, indicating that Syk is important for the sensitisation phase of CHS. <h3>Conclusions</h3> Our results show that Syk is indispensable for the CHS response and suggest a role for this kinase in the sensitisation phase. This is not due to the role of Syk in B-cells but, at least in part, to the expression of Syk in neutrophils.
Internal-Mammary-Artery Ligation for Angina Pectoris
Authors: Robert G. Fish, Thomas P. Crymes, Martha G. Lovell
Journal: New England Journal of Medicine (1958)

EMPHASIS upon the increasing morbidity and mortality associated with coronary-artery disease has led to the proposal of several procedures designed to effect myocardial revascularization. Two technics for this purpose that have attracted wide attention are those advocated by Beck1 and Vineberg and Walker.2 The simplest and most recent surgical procedure that has been suggested as a means of increasing the blood supply to the myocardium is that of bilateral internal-mammary-artery ligation. This operation originated in Italy. Results in 18 cases were reported in 1956 by De Marchi, Battezzati and Tagliaferro.3 An evaluation of this particular technic, from both the experimental . . .
Payment Reform and the Mission of Academic Medical Centers
Authors: Paul F. Griner
Journal: New England Journal of Medicine (2010)

U.S. academic medical centers (AMCs) are facing new challenges to their financial well-being. As payers seek to control health care costs, teaching hospitals and their medical staffs can anticipate continued payment reductions. Under the fee-for-service system, hospitals respond to payment cuts by increasing their volumes of admissions and ambulatory services while improving efficiency. Although costs per case may decline, overall costs do not. The inevitable result is a further reduction in per-case payments, and the cycle continues — with many undesirable consequences. Costs are inflated, and the quality and safety of care are eroded as the result of unnecessary or . . .
Treatment of Bleeding in Hereditary Hemorrhagic Telangiectasia with Aminocaproic Acid
Authors: J R Korzenik, M D Topazian, R White
Journal: New England Journal of Medicine (1994)

Hereditary hemorrhagic telangiectasia (Rendu-Osler-Weber disease) is characterized by telangiectatic lesions of the nose, lips, and visceral organs including the liver, spleen, gastrointestinal tract, lungs, brain, and spinal cord1–5. It is inherited as an autosomal dominant trait. Its most common manifestations are recurrent epistaxis and gastrointestinal bleeding, which can be life threatening6. Nasal bleeding has been treated by dermatoplasty of the nasal septa, photocoagulation, and amniotic-membrane grafts6,7 and by the administration of estrogen and progesterone,8,9 but these treatments are often ineffective. Aminocaproic acid is a potent inhibitor of the fibrinolytic system. In low doses it blocks . . .
Proximal Tubular Malfunction as a Mechanism for Diuresis after Renal Homotransplantation
Authors: L.W. Henderson, K. D. Nolph, J. B. Puschett, M. Goldberg
Journal: New England Journal of Medicine (1968)

Four patients who received renal homografts were studied during the first 24 hours after operation to determine the mechanisms of the post-transplantation diuresis. The marked diuresis was associated with decreased tubular reabsorption of sodium and glucose. Fractional excretion of filtered sodium reached values as high as 0.12 to 0.21, and fractional excretion of glucose achieved values as high as 0.10 at normal plasma glucose levels. The marked natriuresis and glycosuria subsided or improved spontaneously despite meticulous replacement of all renal and extrarenal fluid and electrolyte losses. In two subjects who excreted a hypotonic urine, free water excretion during the natriuresis rose to values as high as 12 to 20 percent of the glomerular filtration rate. Excreted potassium exceeded filtered potassium in two subjects. The data indicate that the early post-transplantation diuresis is due primarily to intrinsic defects in the proximal tubular transport systems for sodium and glucose whereas the transport systems in the distal nephron are relatively intact.
Long-term outcomes and late effects for childhood and young adulthood intracranial germinomas
Authors: Sahaja Acharya, Todd DeWees, Eric T. Shinohara, Stephanie M. Perkins
Journal: Neuro-Oncology (2014)

Pediatric and young adult central nervous system (CNS) germinomas have favorable cure rates. However, long-term follow-up data are limited because of the rarity of this tumor. We report the long-term overall survival (OS) and causes of late mortality for these patients.Data between 1973 and 2005 from the Surveillance, Epidemiology, and End Results (SEER) database were analyzed. Kaplan Meier survival analysis was performed on 5-year survivors of childhood CNS germinomatous germ cell tumors (GGCTs) and nongerminomatous germ cell tumors (NGGCTs). Standardized mortality ratios (SMRs) were calculated using US population data to compare observed versus expected all-cause death and death from stroke. Cumulative incidence was calculated using a competing risk model.Four hundred five GGCTs and 94 NGGCTs cases were eligible. OS at 20 and 30 years for GGCTs was 84.1% and 61.9%, respectively, and was 86.7% for NGGCTs at both time points. Five-year survivors of GGCTs and NGGCTs experienced a 10-fold increase in mortality risk compared with their peers (SMR, 10.41; 95% confidence interval [CI], 7.71-13.76 vs SMR, 10.39;95% CI, 4.83-19.73, respectively). Five-year survivors GGCTs also experienced a nearly 59-fold increase in risk of death from stroke (SMR, 58.93; 95% CI, 18.72-142.10). At 25 years, the cumulative incidence of death due to cancer and subsequent malignancy was 16% and 6.0%, respectively.Although CNS germinomas have favorable cure rates, late recurrences, subsequent malignancies, and stroke significantly affect long-term survival. Close attention to long-term follow-up with assessment of stroke risk factors is recommended.
Case 12-2005
Authors: Margaret A. Shipp, Suzanne L. Aquino, Nancy Lee Harris
Journal: New England Journal of Medicine (2005)

A 30-year-old woman had a two-month history of increasing pain in the left side of her chest and in her arm and shoulder, followed by cough, hemoptysis, and pleuritic chest pain. Chest radiographs showed an infiltrate in the lingula of the left lung. Her symptoms improved minimally with antibiotic therapy. Radiographs of the chest disclosed a large anterior mediastinal mass. A diagnostic procedure was performed.
TCT-231 World’s First Series of Left Main Bifurcation Treated with the AXXESS 4.0 x 9 mm Dedicated Bifurcation Stent
Authors: Omar Rana, Sanjay Sastry, Farzin FATH-ORDOUBADI, Mamas Mamas, Suneel Talwar, Jehangir Din, Peter D. O'Kane
Journal: Journal of the American College of Cardiology (2014)
Standard or ultrathin device for colonoscopy?
Authors: Yufei Wang, Enqiang Linghu
Journal: Endoscopy (2014)

We read with great interest the article by Töx et al. [1]. The authors compared the use of an ultrathin colonoscope (UTC) and a standard colonoscope with regard to demand for propofol during colonoscopy. They found that use of a new type of UTC was associated with less propofol consumption, lower patient sedation levels, and less pain compared with the standard colonoscope. But the time to intubation of the ileum was longer. These findings have relevance for the clinical practice of colonoscopy but are limited in some respects.
Hilar Bronchial Carcinoid Tumor
Authors: E Koh, A Patrikeos, R Steens, P Robins
Journal: Clinical Nuclear Medicine (2001)

KOH, EAMON M.B.B.S.*; PATRIKEOS, ANDREW M.B.B.S., M.R.C.P., F.R.A.N.Z.C.R.*; STEENS, RODNEY M.B.B.S., F.R.A.C.P.†; ROBINS, PETER M.B.B.S., F.R.A.N.Z.C.R., A.N.Z.A.P.N.M.* Author Information
A prospective, randomized trial of chemotherapy with or without the low molecular weight heparin (LMWH) enoxaparin in patients (pts) with advanced pancreatic cancer (APC): Results of the CONKO 004 trial
Authors: H. Riess, U. Pelzer, G. Deutschinoff, B. Opitz, M. Stauch, P. Reitzig, S. Hahnfeld, A. Hilbig, J. Stieler, H. Oettle
Journal: Journal of Clinical Oncology (2009)

LBA4506 The full, final text of this abstract will be available in Part II of the 2009 ASCO Annual Meeting Proceedings, distributed onsite at the Meeting on May 30, 2009, and as a supplement to the June 20, 2009, issue of the Journal of Clinical Oncology. No significant financial relationships to disclose.
Abstract 4772: A novel agonist antibody to human death receptor 4, AY4, induces apoptotic cell death in head and neck cancer through ROS generation: <i>in vitro</i> and <i>in vivo</i>
Authors: Chul-Ho Kim, Bok-Soon Lee, Sung Un Kang, Hye Sook Hwang, Eun-Sil Sung, Yong-Sung Kim, Yoon Woo Koh, Hyun Jun Hong, Mi Ran Ryu, Jae Wook Kim
Journal: Cancer Research (2011)

Abstract Background : The proapoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) receptors death receptor (DR) 4 and DR5 are attractive targets to develop the receptorspecific agonistic monoclonal antibodies (mAb) as anticancer agents because of their tumor-selective cell death-inducing activity. Here, we describe the characterization of a novel agonistic mAb (AY4) against human DR4 and apoptosis through reactive oxygen species (ROS) production in head and neck cancer model. Methods : The effects of AY4 in head and neck cell lines (KB, SNU899, HN9, FaDu) was studied on apoptosis using MTT assy, TUNEL, Annexin V assay and western blotting and was examed on the production of reactive oxygen species with DCFDA staining, intracellular signaling and animal model. In addition, we analyzed the anticancer effect of AY4 in nude mice xenograft model. In addition, drug toxicity (embryotoxicity and neurotoxicity) of AY4 were investigated in vivo zebrafish model. Results: The DR4 was expressed in head and neck cancer cell lines by Flow cytometry (FACS) and western blotting. AY4 inhibited cell growth in KB and HN9 excluding SNU899 and FaDu and caused cell death via caspase-dependent apoptosis. The apoptotic cell death by AY4 decreased by N-acetylcysteine (NAC), an antioxidant, suggesting ROS generation involved in the cell death. Moreover, NAC and AY4 cotreatment augmented the reduced expression of antiapoptotic molecules, Bcl-XL and XIAP. In vivo administration of AY4 significantly inhibited tumor growth of head and neck cancer preestablished in athymic nude mice. In addition, in a zebrafish model used for toxicity testing, a considerable dose of AY4 did not result in embryotoxicity or neurotoxicity. Conclusion : Our results demonstrates the effect of AY4 on apoptosis and tumor inhibition via ROS production and provide potential use of AY4 as an anticancer therapeutic agent in head and neck cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4772. doi:10.1158/1538-7445.AM2011-4772
Abstract 3464: Epigenetic mechanism is involved in depleted uranium-induced transformation in human lung epithelial cells
Authors: Hong Xie, Carolyne LaCerte, John P. Wise
Journal: Cancer Research (2010)

Abstract Depleted uranium (DU) is commonly used in military applications and is also used in civilian industry and thus exposure of soldiers and others is frequent and widespread. There is limited research information on the potential health hazards of DU exposure. In our study, we used the human bronchial epithelial cell line (BEP2D) to study the potential carcinogenic effects of DU. DNA strand breaks were observed at 25 ug/cm2 DU treatment. We found that DU induces cell transformation including cell contact inhibition and anchorage independent phenotype. Doses of 0.25, 2.5 and 25 ug/cm2 uranium trioxide induces 23, 24 and 17 growth foci in 30 dishes, respectively. These foci were cloned and were found to exhibit anchorage independent growth and an aneuploid phenotype. We next investigated DU-induced epigenetic modifications. We found that DU decreases trimethylated H3K9 (H3K9me3), along with inhibition of the DNA damage repair protein ATM. Our study demonstrates that DU-induced both genotoxicity and aberrant histone methylation which may contribute to its carcinogenesis. This work was supported by ARO grants # W911NF-04-1-0240 and W911NF-08-0333 (J.P.W.). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3464.