tbuckley/cabot-search huggingface dataset:
Immunologic and Enzymatic Properties of a Cell Line Derived From a Mouse Thymic Tumor&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: N M Munsch, J P Kolb
Journal: JNCI Journal of the National Cancer Institute (1980)

Nu8, a T-cell tumor line derived from a thymic tumor induced in an inbred IC mouse treated with 7,12-dimethyl-benz[a]anthracene, was established and characterized in vitro. The Nu8 were rapidly dividing cells, nonadherent to nylon wool, and killed by an anti-Thy 1 antiserum and also by a xenogenic anti-T-cell antiserum; inasmuch as they also did not bear surface immunoglobulin, as judged by membrane immunofluorescence, their T-cell nature was demonstrated. They were highly sensitive to the action of corticosteroids in vitro and possessed terminal deoxynucleotidyl transferase (TdT), an enzyme essentially located in the thymus cortex and a marker of acute lymphoblastic leukemia. Nu8 cells were negative for the peanut agglutinin marker and also for rosette formation with autologous erythrocytes, which suggested that they were not post-thymic precursor T-cells. They lacked the markers of committed mature T-cells, such as the receptors for the Fc part of IgM or IgG. They were therefore very immature T-cells. Because of their high rate of multiplication, their stimulation with classical T-cell mitogens could not be tested. They did have the membrane structure of tumor cells passaged in vitro, inasmuch as they were agglutin-able with concanavalin A and were good targets of natural killer activity. The homogeneity of the Nu8 cells was assessed by the fact that after density gradient centrifugation, a single-peak distribution of the cells was obtained with a constant TdT activity. In conclusion, this tumor cell line had immature T-cell characteristics similar to those described for certain groups of human acute lymphoblastic leukemias.
Behavioral changes following surgery for obesity
Authors: M J Mills, A J Stunkard
Journal: American Journal of Psychiatry (1976)

The authors interviewed 69 subjects who had had jejuno-ileal bypass surgery for obesity to determine the relationship between weight loss and eating behavior after surgery. All patients had been extremely overweight; at an average of 37 months after surgery, their mean weight was reduced by 39%. There were four significant postoperative changes: voluntary restriction of food intake, "normalization" of eating patterns, resistance to eating during intense emotion, and far less dysphoria during postsurgical weight loss than during previous weight loss efforts. The authors therefore suggest that factors other than impaired intestinal absorption may account for some portion of postsurgical weight loss.
Patient-Derived Xenografts Accurately Capture Clinical Responses to Treatment
Authors: J. Stebbing, S. Zacharoulis, A. Gaya, W. McGuire, W. Harris, R.G. Maki, M. Hidalgo, A. Davies, D. Vasquez-Dunddel, D. Ciznadija, A. Katz, D. Sidransky, K. Paz
Journal: Annals of Oncology (2014)
Abstract 123: Chest Stiffness: Getting to a 6 cm Compression Depth Expectation
Authors: Paul Aelen, Igor Paulussen, Gerrit-Jan Noordergraaf, Pierre Woerlee
Journal: Circulation (2013)

Introduction: AHA guidelines suggest that it’s reasonable for healthcare providers to perform CPR with compression depths of at least 5 cm. However, forces needed to reach these depths can be large...
Estrogen-mediated downregulation of AIRE influences sexual dimorphism in autoimmune diseases
Authors: Nadine Dragin, Jacky Bismuth, Géraldine Cizeron-Clairac, Maria Grazia Biferi, Claire Berthault, Alain Serraf, Rémi Nottin, David Klatzmann, Ana Cumano, Martine Barkats, Rozen Le Panse, Sonia Berrih-Aknin
Journal: Journal of Clinical Investigation (2016)

Autoimmune diseases affect 5% to 8% of the population, and females are more susceptible to these diseases than males. Here, we analyzed human thymic transcriptome and revealed sex-associated differences in the expression of tissue-specific antigens that are controlled by the autoimmune regulator (AIRE), a key factor in central tolerance. We hypothesized that the level of AIRE is linked to sexual dimorphism susceptibility to autoimmune diseases. In human and mouse thymus, females expressed less AIRE (mRNA and protein) than males after puberty. These results were confirmed in purified murine thymic epithelial cells (TECs). We also demonstrated that AIRE expression is related to sexual hormones, as male castration decreased AIRE thymic expression and estrogen receptor α-deficient mice did not show a sex disparity for AIRE expression. Moreover, estrogen treatment resulted in downregulation of AIRE expression in cultured human TECs, human thymic tissue grafted to immunodeficient mice, and murine fetal thymus organ cultures. AIRE levels in human thymus grafted in immunodeficient mice depended upon the sex of the recipient. Estrogen also upregulated the number of methylated CpG sites in the AIRE promoter. Together, our results indicate that in females, estrogen induces epigenetic changes in the AIRE gene, leading to reduced AIRE expression under a threshold that increases female susceptibility to autoimmune diseases.
ATPS-42INHIBITION OF STAT3 ENHANCES THE RADIOSENSITIZING EFFECT OF TEMOZOLOMIDE IN MALIGNANT GLIOMA CELLS IN VITRO AND IN VIVO
Authors: Tae Jin Han, Eun Jung Choi, Bong Jun Cho, Sang Hyuk Song, Sun Ha Paik, In Ah Kim
Journal: Neuro-Oncology (2015)

PURPOSE: Despite aggressive treatment with radiation therapy plus concurrent and adjuvant temozolomide (TMZ), the prognosis for glioblastoma remain poor. We investigated the potential of targeting signal transducer and activator of transcription-3 (STAT3) to improve the therapeutic outcome of combined radiotherapy and TMZ in glioblastoma. METHODS AND MATERIALS: We evaluated the preclinical potential of a STAT3 inhibitor, Cpd188 combined with temozolomide and radiation by in vitro clonogenic assays using two established glioblastoma cell lines (U251, U87) and two patients-derived glioblastoma cell lines (GBL12, GBL28) and in vivo studies using nude mice bearing intracranial U251 xenografts. RESULTS: Cpd188 potentiated the radiosensitizing effect of TMZ in U251 glioblastoma cell line which has high levels of p-STAT3 expression and in vitro. Increased radiosensitizing effects of TMZ were associated with the induction of apoptosis and the reversion of epithelial-mesenchymal transition (EMT). Cpd188 delayed in vivo tumor growth both alone and in combination with fractionated radiation and TMZ with a trend toward improved survival rates. Immunohistochemical staining of tumor sections showed that Cpd188 decreased the expression of CD31 (a marker of endothelial proliferation), vascular endothelial growth factor, and hypoxia-inducible factor-1α, suggesting that Cpd188 also has anti-angiogenic effects. We also confirmed the radiosensitizing effect of Cpd188 of GBL28 cell line which was originated from a patient who had a glioblastoma and also was confirmed high level of STAT3 expression and unmethylated MGMT. CONCLUSION: These data indicate that Cpd188 has the potential to improve the therapeutic outcome of combined radiotherapy and TMZ in human glioblastoma, especially in patients whose tumor has a high level of STAT3 expression regardless of MGMT methylation status. Work supported by grant (#2012-0004867 & #2013R1A1A2074531) from National Research Foundation, Korean Ministry of Future Creative Science to In Ah Kim.
A retrospective analysis of treatment in triple-negative brest cancer (TNBC)
Authors: M. Popovich, V. Russu, B. Lex, A. H. Tulusan
Journal: Journal of Clinical Oncology (2009)

e11623 Background: TNBC makes about 12.5% of all breast cancer. TNBC show have a poor prognosis due to the aggressive biology with relative resistance to systemic therapies. 85% of TNBC are basal-like subtype. Basal-like TNBC are diagnosed by a positive CK5/14 stainig in immunohistochemical staining. Methods: 200 pts with TNBC in Bayreuth hospital were analysed retrospectively between 1995 and 2006. 52/200 (26%) pts underwent primary systemic therapy (PST). 6/200 pts primary had stage IV disease. In 49 pts ck 5/14 staining was done. Results: The median follow up 56 mts, median age at diagnosis was 52 yrs. At the time of analysis 146/200 pts were alive. DFS was 25 mts, OS 44 mts. 7/42 (16. 6%) had a pathological complete response rate (pCR) after PST, in 5/7 pts PST was based on anthracyclines. The estimated residual tumor size in preoperative MR correlated strongly with the histopathological tumor size (Pearson Correlation r=0,81, p&lt; 0.01). Stage IV patients with viszeral disease had a median age of 69 yrs and median OS 6,3 (2–12 mts). Overall recurrence rate was 24% (48/200 pts). Grading in all TNBC variated (G1: 7 pts (3,5%), G2: 52 pts (26%), G3: 132 pts (66%). Conclusions: The retrospective data demonstrate that basal-like TNBC is an important subgroup with a poor prognosis. In future it will be important to pick up these subgroup to bring them to different therapies. Preoperative MR is a good tool to predict the residual tumor size after PST in all TNBC pts. Anthracycline-based systemic therapy is still standard in adjuvant treatment. No significant financial relationships to disclose.
P-142 Severity of Malnutrition Status of Gastrointestinal Cancer: Nutritional Screening and Assessment Registry in Korean Gastroenterointestinal Cancer Patients
Authors: E.S. Kim, G.A. Song, D.U. Kim, S.E. Kim, H.S. Moon, S.H. Lee, M.J. Chung, S.Y. Park, S.H. Kim
Journal: Annals of Oncology (2015)
Non-randomised Ebola trials—lessons for optimal outbreak research
Authors: Giuseppe Ippolito, Simone Lanini, Philippe Brouqui, Antonino Di Caro, Francesco Vairo, Francesco Maria Fusco, Sanjeev Krishna, Maria Rosaria Capobianchi, Henry Kyobe-Bosa, Vincenzo Puro, Roman Wölfel, Tatjana Avsic-Zupanc, John P A Ioannidis, Gina Portella, Peter Kremsner, Osman Dar, Matthew Bates, Alimuddin Zumla
Journal: The Lancet Infectious Diseases (2016)
The effect of state anxiety on personality measurement
Authors: J Reich, R Noyes, W Coryell, T W O'Gorman
Journal: American Journal of Psychiatry (1986)

The authors examined the effect of state anxiety on the personality test scores of 56 patients receiving treatment for panic disorder and agoraphobia. The tests were administered before treatment and again 6 weeks later. For the 40 patients who improved by 5 or more points on the Hamilton Anxiety Rating Scale, significant changes in personality measures were observed at week 6, including increased emotional strength and extraversion and decreased interpersonal dependency. The authors conclude that state anxiety, like depression, is a possible confounding factor in personality measurement, and adjustment for it should be made in future studies.
Neural Differentiation and Prognosis in Peripheral Primitive Neuroectodermal Tumor
Authors: Leonard H. Wexler, William H. Meyer, David M. Parham, Maria Tsokos
Journal: Journal of Clinical Oncology (2000)

Article Tools SPECIAL DEPARTMENTS Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2000.18.10.2187 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 10811686 Neural Differentiation and Prognosis in Peripheral Primitive Neuroectodermal Tumor Leonard H. WexlerxLeonard H. WexlerSearch for articles by this author , William H. MeyerxWilliam H. MeyerSearch for articles by this author , David M. ParhamxDavid M. ParhamSearch for articles by this author , Maria TsokosxMaria TsokosSearch for articles by this author Show More Memorial Sloan-Kettering Cancer Center New YorkNYUniversity of Oklahoma Health Science Center Oklahoma CityOKArkansas Children’s Hospital Little RockARNational Cancer Institute BethesdaMD https://doi.org/10.1200/JCO.2000.18.10.2187 First Page Full Text PDF Figures and Tables © 2000 by American Society of Clinical Oncology
Behavioral and biological effects of acute beta-endorphin injection in schizophrenic and depressed patients
Authors: D Pickar, G C Davis, S C Schulz, I Extein, R Wagner, D Naber, P W Gold, D P van Kammen, F K Goodwin, R J Wyatt, C H Li, W E Bunney
Journal: American Journal of Psychiatry (1981)

In this double-blind study, beta-endorphin, 4-15 mg, was administered intravenously to 6 schizophrenic and 4 depressed patients. There were neither significant differences in behavioral ratings between beta-endorphin and placebo for the overall group nor for either the schizophrenic or depressed subgroup. Clinical worsening and improvement were observed in individual schizophrenic patients. There was no evidence of late-appearing therapeutic effects in 4 schizophrenic patients rated for 5 consecutive days after placebo and drug infusions. In 1 patient 10 mg of beta-endorphin produced neuroendocrine effects comparable to those produced by 5 mg of intravenously administered methadone; in 2 other patients it produced large increases in circulating opioid activity as determined by radioreceptor assay. These biological data support the notion that parenterally administered beta-endorphin exerts significant opiate-like activity in vivo.
Hypomagnesaemia in Cancer Patients: Underestimated Problem? When Supportive Care is Detrimental: the Example of Protonic Pump Inhibitors
Authors: E.S. Traverso, P. Varese, I. Angelini, C. Cosentino, C. Simoni, E. Bennicelli, F. Prasso, F. Paolieri, L. Pizzo, M. Petronio, M. Zucchi, P. Altmura
Journal: Annals of Oncology (2014)
Abstract 202: Elevated Plasma Levels of Interleukin 6 Are Associated with Risk of Sudden Death in the Community Even When Measured During the Acute Cardiac Arrest Event
Authors: Rasmus Havmoeller, Audrey Uy-Evanado, Carmen Teodorescu, Katherine Jerger, Dorothy Kwok, Jo Ayala, Adriana Huertas-Vazquez, Ida Chen, Kyndraron Reinier, Karen Gunson, Jonathan Jui, Sumeet S Chugh
Journal: Circulation (2012)

Introduction: Elevated plasma levels of interleukin 6 (IL6) reflect plaque vulnerability and have been associated with sudden death in a cohort study but samples were drawn many years prior to the event. We sought to confirm this relationship by evaluating IL6 at the time of the sudden cardiac arrest (SCA) in a group of men and women in the community. Hypothesis: Elevated IL6 is a determinant of SCA in the community. Methods: In an ongoing, prospective, community-based study of SCA in Northwest US, we compared levels of plasma IL6 in 143 adult cases of adjudicated SCA and 149 gender- and age-matched controls with coronary artery disease (73 vs 73% male, age 64.0±11.9 vs. 64.2±11.6 y). Cases with cancer, non-cardiac causes of arrest, or terminal illnesses were excluded. Plasma was obtained from blood drawn at the time of arrest (cases) and at a routine visit (controls), and analyzed using Quantikine Immunoassay (CV &lt;7.8%, normal range 0.4-8.9 pg/ml). IL6 levels were log transformed and compared (T-test and Chi-square test) per quartiles (based on controls). Logistic regression was used to predict the adjusted odds of SCA associated with IL6 levels (per increase in quartile). Results: IL6 was significantly higher in SCA cases vs controls (median 13.2 [IQR 5.4-25.8] vs 5.3 [1.5-4.6] pg/ml, P&lt;0.0001). Cases and controls did not differ regarding BMI, smoking, diabetes or obesity (all P&gt;0.05). Median CRP levels were below clinical levels of significance and similar between cases and controls (2.5 [1.0-5.4] vs 1.9 [1.0-6.2] ug/ml, P=0.56). In a logistic regression model IL6 was independently associated with increased risk of SCA (OR/increased quartile of IL6: 2.4, 95% CI 1.7 - 3.5, P&lt;0.001) also after adjusting for other risk factors (Table). Conclusions: Elevated levels of IL6 in plasma are associated with SCA in this community-based study. Further evaluations are needed to elucidate the background of this finding and the potential role of this biomarker in risk stratification of SCA.
Lymphatic Leakage in a Renal Transplant: The Role of Lymphoscintigraphy
Authors: Meltem ??a??lar, Eser Lay Erg??n
Journal: Clinical Nuclear Medicine (2006)

Lymphatic leak is a common cause of perirenal fluid collection in renal transplant patients in whom early recognition and therapy is essential for the survival of the kidney. Nuclear medicine offers dynamic renal scintigraphy for the evaluation of renal perfusion, function, and recognition of urologic complications. We present a renal transplant recipient in whom renal scintigraphy showed abnormal radiotracer collection adjacent to the bladder, which caused urinary stasis in the renal pelvis. Lymphoscintigraphy, which can be used to identify lymphatic collections, revealed abnormal radiotracer accumulation in the transplant fossa and confirmed that the perirenal fluid collection was the result of a lymphatic leak. We have described a patient with perirenal fluid collection after renal transplantation surgery in whom lymphoscintigraphy was used to confirm the diagnosis.
IMPS-09COMBINATION OF DELTA-24-RGDOX AND ANTI-PDL1 ANTIBODIES INDUCES ANTI-TUMOR EFFECT AND ANTI-GLIOMA IMMUNE MEMORY
Authors: Hong Jiang, Candelaria Gomez-Manzano, Frederick Lang, Frank Tufaro, W.K. Alfred Yung, Raymond Sawaya, Juan Fueyo
Journal: Neuro-Oncology (2015)

Immune checkpoint modulators are being tested as anti-glioma therapy in clinical trials. In this work, we combined an oncolytic adenovirus expressing the OX40L, called Delta-24-RGDOX, with the intracranial injection of antibodies against PDL-1. We first observed that infection of murine and human glioma cells with oncolytic viruses triggered immunogenic cell death by assessing secretion of ATP, and the cell death–associated release of the HMGB1 by infected cells using ELISA and Western blot. Cells infected with Delta-24-RGDOX expressed OX40L in in vitro and in vivo conditions, as assessed by flow cytometry. Direct injection of Delta-24-RGDOX into murine gliomas increased animal survival in an immune competent murine model (p < 0.0001), but not in immune deficient mice (p = 1.0). Flow cytomeric analyses of brain infiltrating lymphocytes in the immune competent model showed an overrepresentation of CD4+ (p < 0.0001) and CD8+ cells (p< 0.0001). In addition, histologic examination of the brains revealed tumor necrosis in the immune competent mice treated with Delta-24-RGDOX, but not in the immune deficient model. Importantly, expression of OX40L within the tumor did not result in brain or systemic toxicity. Rechallenging of survivors with implantation of gliomas jeopardized the growth of tumors in the majority of mice. Spleens from mice treated with Delta-24-RGDOX harbored cells that recognized untreated glioma cells. Treating these co-cultures with antibodies against OX40L prevented this immune response. Using OVA as a model antigen we demonstrated that after Delta-24-RGDOX infection, CD8 cells specifically recognized cellular antigens. Importantly, combination of treatment of Delta-24-RGDOX and anti-PDL1 antibodies resulted in 100% survival of glioma-bearing immune competent mice. Our results indicate that is feasible to combine the inflammatory and lytic properties of oncolytic viruses with the modulation of immune checkpoints. Combination of several immune checkpoints modulators and oncolytic viruses may be required to obtain an effective anti-glioma therapy in a majority of patients.
Nasal high flow oxygen therapy in patients with COPD reduces respiratory rate and tissue carbon dioxide while increasing tidal and end-expiratory lung volumes: a randomised crossover trial
Authors: John F Fraser, Amy J Spooner, Kimble R Dunster, Chris M Anstey, Amanda Corley
Journal: Thorax (2016)

<h3>Abstract</h3> Patients with COPD using long-term oxygen therapy (LTOT) over 15 h per day have improved outcomes. As inhalation of dry cold gas is detrimental to mucociliary clearance, humidified nasal high flow (NHF) oxygen may reduce frequency of exacerbations, while improving lung function and quality of life in this cohort. In this randomised crossover study, we assessed short-term physiological responses to NHF therapy in 30 males chronically treated with LTOT. LTOT (2–4 L/min) through nasal cannula was compared with NHF at 30 L/min from an AIRVO through an Optiflow nasal interface with entrained supplemental oxygen. Comparing NHF with LTOT: transcutaneous carbon dioxide (TcCO<sub>2</sub>) (43.3 vs 46.7 mm Hg, p&lt;0.001), transcutaneous oxygen (TcO<sub>2</sub>) (97.1 vs 101.2 mm Hg, p=0.01), I:E ratio (0.75 vs 0.86, p=0.02) and respiratory rate (RR) (15.4 vs 19.2 bpm, p&lt;0.001) were lower; and tidal volume (Vt) (0.50 vs 0.40, p=0.003) and end-expiratory lung volume (EELV) (174% vs 113%, p&lt;0.001) were higher. EELV is expressed as relative change from baseline (%Δ). Subjective dyspnoea and interface comfort favoured LTOT. NHF decreased TcCO<sub>2</sub>, I:E ratio and RR, with a concurrent increase in EELV and Vt compared with LTOT. This demonstrates a potential mechanistic rationale behind the improved outcomes observed in long-term treatment with NHF in oxygen-dependent patients. <h3>Trial registration number</h3> ACTRN12613000028707.
Propranolol improves endothelial dysfunction in advanced cirrhosis: the ‘endothelial exhaustion’ hypothesis
Authors: Anderson Brito-Azevedo, Renata de Mello Perez, Henrique Sérgio Moraes Coelho, Eduardo de Souza Martins Fernandes, Raquel Carvalho Castiglione, Cristiane Alves Villela-Nogueira, Eliete Bouskela
Journal: Gut (2016)

We read with interest the recent work of Leithead and coworkers about the use of non-selective β-blockers (NSBBs) in patients in the transplant waiting list. They studied 322 patients, among those 117 had refractory ascites and observed higher survival rate in those using NSBB, including the ones with refractory ascites (HR mortality=0.35; confidence interval (CI) 95%: 0.14 to 0.86).1 However, the use of NSBB has been a polemic issue since the hypothesis proposed by Krag and coworkers suggesting the existence of a ‘therapeutic window’, according to which NSBB would not be beneficial to patients in early or end-stage cirrhosis with refractory ascites, when it would impair the compensatory cardiac output increase, worsening organ perfusion in decompensated cirrhosis.2

Considering current controversies about the use of propranolol (PPL) in advanced cirrhosis, and its possible deleterious effect on haemodynamic and organ perfusion, we have investigated the endothelial function in patients with …
Cell Culture From a Rat Brain Tumor Induced by Intracerebral Inoculation With Murine Sarcoma Virus2
Authors: Noriaki Ida, Yoji Ikawa, Kayata Ogawa, Michiko Takada, Haruo Sugano
Journal: JNCI Journal of the National Cancer Institute (1974)

A cell culture line of possible glial cell origin was established from a brain tumor induced in the rat by murine sarcoma virus (MSV)-Moloney (congenitally or vertically transmitted). The cultured cells, globose or pear-shaped with protoplasmic processes and highly refractile membranes, were transplantable successively in suckling rats by intracerebral, subcutaneous, or intramuscular injection. Young rats bearing the transplanted subcutaneous tumor showed metastases to lungs, liver, spleen, kidneys, regional lymph nodes, and thymus. Cell-free extracts of cultured cells induced brain tumors or sarcomas in newborn rats and mice. The results of focus formation by supernatants of cell culture and electron microscopic findings of extracellular and intercellular viral particles also indicated that cultured glial cells produced infectious MSV.
BRAF inhibition in hairy cell leukemia with low-dose vemurafenib
Authors: Sascha Dietrich, Andreas Pircher, Volker Endris, Frédéric Peyrade, Clemens-Martin Wendtner, George A. Follows, Jennifer Hüllein, Alexander Jethwa, Elena Ellert, Tatjana Walther, Xiyang Liu, Martin J. S. Dyer, Thomas Elter, Tilman Brummer, Robert Zeiser, Michael Hermann, Michael Herold, Wilko Weichert, Claire Dearden, Torsten Haferlach, Martina Seiffert, Michael Hallek, Christof von Kalle, Anthony D. Ho, Anita Gaehler, Mindaugas Andrulis, Michael Steurer, Thorsten Zenz
Journal: Blood (2016)