tbuckley/cabot-search huggingface dataset:
Alterations of GPI transamidase subunits in head and neck squamous carcinoma
Authors: Wei-Wen Jiang, Marianna Zahurak, Zeng-Tong Zhou, Hannah Lui Park, Zhong-Min Guo, Guo-Jun Wu, David Sidransky, Barry Trink, Joseph A Califano
Journal: Molecular Cancer (2007)

Abstract Background GPI anchor attachment is catalyzed by the GPI transamidase (GPIT) complex. GAA1, PIG-T and PIG-U are the three of five GPIT subunits. Previous studies demonstrated amplification and overexpression of GPIT subunits in bladder and breast cancer with oncogenic function. We performed an analysis of these subunits in head and neck squamous cell carcinoma (HNSCC). Results To evaluate GAA1, PIG-T and PIG-U in HNSCC, we used quantitative PCR (QPCR) and quantitative RT-PCR (QRT-PCR) to determine the copy number of those genes in primary tumors and the matching lymphocytes in 28 patients with HNSCC and quantified RNA expression of those genes in 16 primary HNSCC patients and 4 normal control tissue samples. GAA1 showed a significant increase in normalized mRNA expression, 2.11 (95% CI: 1.43, 2.79), in comparison to that of normal controls, 0.43 (95% CI: -0.76, 1.61), p = 0.014 ( Mann-Whitney test ). The mean genomic copy number of GAA1 was significantly increased in HNSCC, 0.59 (95% CI: 0.50, 0.79), in comparison to lymphocyte DNA, 0.35 (95% CI: 0.30, 0.50), p = 0.001 (paired t-test) . Conclusion An increased expression level and elevated copy number for GAA1 suggest a role for this GPI anchor subunit in HNSCC.
Competitors Try Collaboration To Speed Drug Development
Authors: Margie Patlak
Journal: JNCI Journal of the National Cancer Institute (2010)

Sharing data may be a venerable tradition in some research communities, but nobody in the world of drug development expects to collaborate with potential competitors. That situation may be changing, though, according to speakers at a workshop on precompetitive collaboration in cancer research, sponsored by the Institute of Medicine (IOM) last February. The hope is that such collaborations, just now getting under way and involving both academic and industry researchers, will boost the currently dismal 5% success rate for new anticancer compounds in drug development pipelines.
Recurrent Haemorrhage from Iatrogenic Left Ventricular Aneurysm
Authors: S. R. Panday, G. B. Parulkar, M. D. Kelkar, P. K. Sen
Journal: Thorax (1965)

Most of the literature on penetrating wounds of the heart is concerned with the diagnosis and management of acute cardiac tamponade, but very scant attention seems to have been paid to its sequelae and complications.Cardiac aneurysm is now increasingly being recognized as a complica- tion following accidental wounds, incisions into the heart for introducing instruments, and open- heart correction of certain cardiac defects.In this communication a report of a case of traumatic ventricular aneurysm following accidental incision into the left ventricle is presented, because of the unusual circumstances that led to its production and its mode of presentation.CASE REPORT B. R., a boy aged 10 years, was admitted to the K.E.M. Hospital, Bombay, on 4 February 1963 for reduction under general anaesthesia of a slipped epiphysis of the lower end of the right radius.During induction of anaesthesia with ethyl chloride the child developed cardiac arrest.Immediate closed cardiac massage was attempted for about one and a half minutes without any favourable response.The child was intubated, and the chest was opened through the fourth left intercostal space.While opening the chest in haste there was an accidental injury to the peri- cardium and left ventricular myocardium.The incised on August 2,
Accuracy of the i-STATTM bedside blood gas analyser
Authors: S Sediame, F Zerah-Lancner, M P d'Ortho, S Adnot, A Harf
Journal: European Respiratory Journal (1999)

The performance of the i-STAT portable clinical analyser for measuring blood gases and pH was evaluated with reference to a conventional blood gas analyser (ABL520 Radiometer). Ninety-two samples from the routine blood gas analysis laboratory were chosen according to a wide distribution of partial pressure of carbon dioxide (Pa,CO2), partial pressure oxygen (Pa,O2) and pH and then analysed. All measurements were performed in duplicate by trained technicians from the central hospital laboratory. Differences between duplicate measurements were computed for Pa,CO2: (1.2 versus 0.4%), Pa,O2 (1.7 versus 1.1%) and pH (0.06 versus 0.02%), for the i-STAT and ABL520, respectively. pH and Pa,CO2 values measured with the i-STAT were very close to those obtained with the ABL520, the difference (mean+/-SD) being 0.006+/-0.018 and -0.13+/-0.17 kPa, respectively. Statistical analysis showed that the differences between analysers did not depend on values of pH or Pa,CO2. The performance of the analysers depended on the level of PO2. Below 15 kPa (n=48), the two systems gave nearly identical values, the mean difference was 0.01+/-0.37 kPa. Between 16 and 55 kPa (n=44), there was a systematic but small (-0.69+/-0.67 kPa) underestimation of Pa,O2 measured with the i-STAT (p<10(-8)). In conclusion, this study shows that blood gas analysis using the i-STAT portable device is comparable with that performed by a conventional laboratory blood gas analyser.
Cellular electrolyte and volume changes induced by acidosis in the rabbit proximal straight tubule.
Authors: L P Sullivan, D P Wallace, R L Clancy, C Lechene, J J Grantham
Journal: Journal of the American Society of Nephrology (1991)

Cellular acidosis induced either by high Pco2 or by low HCO3- concentrations has been shown to cause cell swelling in isolated, lumen-collapsed, S2 segments of the rabbit proximal tubule (Sullivan et al., Am J Physiol 1990; 258: F831-F839). The swelling is not followed by a volume regulatory response. The ionic basis of the swelling has been investigated by measurement of the cellular K+, Na+, and Cl- content (electron probe) and HCO3- concentration (pH-sensitive fluorescent dye). Cell content of K+, Na+, and Cl- was expressed as a ratio to P content. Exposure to 15% CO2 increased K/P from 0.98 to 1.16, Cl/P from 0.14 to 0.20, and Na/P from 0.09 to 0.11. Cell (HCO3-) increased from 22 to 32 mM. Reduction in bath (HCO3-) from 25 to 5 mM reduced cell (HCO3-) from 24 to 8 mM and increased K/P from 0.75 to 0.90. Na/P fell from 0.13 to 0.09, and Cl/P fell from 0.15 to 0.12. Thus, swelling resulting from acidosis induced by high CO2 was accompanied by an accumulation of K+, Cl-, and HCO3-; that resulting from acidosis induced by a fall in (HCO3-) was combined with an accumulation of K+ and an unidentified anion. To determine if the swelling induced by a fall in pH might be coupled with depolarization of the basolateral membrane, the effect of 1 mM barium was tested. Barium caused cell volume to increase 10.2%. Cell pH rose from 7.38 to 7.56, K/P increased from 0.63 to 0.73, Na/P did not change, and Cl/P rose from 0.17 to 0.20. Cell (HCO3-) increased 10.4 mM. When the pH of the barium-treated tissue was reduced to 7.02 by raising Pco2, additional cell swelling and accumulation of K+ occurred. The effect on cell volume of a reduction of bath (HCO3-) from 25 to 5 mM at constant bath pH was determined. Cell pH was not altered. Cell volume decreased 3% initially and then returned to the control level. When the bath (HCO3-) was restored to 25 mM, cell volume increased 3.9% and then returned to the baseline. Thus, volume regulation was not impaired. It was concluded that a fall in cell pH induces swelling, and this is coupled with an accumulation of K+. This is probably the result of a pH effect on barium-sensitive and barium-insensitive K+ conductance pathways. The nature of the anions that balance the gain in K+ depends on the means used to induce acidosis.
A new integrative model of lateral epicondylalgia: Figure 1
Authors: B K Coombes, L Bisset, B Vicenzino
Journal: British Journal of Sports Medicine (2008)

Tennis elbow or lateral epicondylalgia is a diagnosis familiar to many within the general community and presents with an uncomplicated clinical picture in most cases. However, the underlying pathophysiology presents a more complex state and its management has not been conclusively determined. Research on this topic extends across anatomical, biomechanical and clinical literature; however, integration of findings is lacking. We propose that the current understanding of the underlying pathophysiology of lateral epicondylalgia can be conceptualised as encompassing three interrelated components: (i) the local tendon pathology, (ii) changes in the pain system, and (iii) motor system impairments. This paper presents a model that integrates these components on the basis of a literature review with the express aim of assisting in the targeting of specific treatments or combinations thereof to individual patients.
A NOTE ON NITROUS OXIDE AS AN ANESTHETIC IN ANIMAL EXPERIMENTATION
Authors: David H. Dolley
Journal: The Journal of Experimental Medicine (1914)

Nitrous oxide, administered by means of the Gatch apparatus, as a preliminary anesthetic to ether has been found to be superior for the dog in rapidity of action, in promotion of ease of handling, and in absence of disorder; the anesthesia may be maintained solely by nitrous oxide in combination with oxygen; it is economical; and its use is instructive to students from closer imitation of current surgical methods.
Enterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's Patches
Authors: Karen Laky, Leo Lefrançois, Elizabeth G. Lingenheld, Hiromichi Ishikawa, Julia M. Lewis, Sara Olson, Kenji Suzuki, Robert E. Tigelaar, Lynn Puddington
Journal: The Journal of Experimental Medicine (2000)

The intestinal mucosa is suggested to support extrathymic T cell development, particularly for T cell receptor (TCR)-gammadelta intraepithelial lymphocytes (IELs). TCR-gammadelta cell development requires interleukin (IL)-7; IL-7(-/)- or IL-7 receptor(-/)- mice lack TCR-gammadelta cells. Using the intestinal fatty acid binding protein (iFABP) promoter, we reinstated expression of IL-7 to mature enterocytes of IL-7(-/)- mice (iFABP-IL7). In iFABP-IL7 mice, TCR-gammadelta IELs were restored, as were cryptopatches and Peyer's patches. TCR-gammadelta cells remained absent from all other tissues. Likewise, T cell development in thymus and B cell maturation in the bone marrow and spleen retained the IL-7(-/)- phenotype. Thus, IL-7 expression by enterocytes was sufficient for extrathymic development of TCR-gammadelta cells in situ within the intestinal epithelium and was crucial for organization of mucosal lymphoid tissue.
Effects of plant density of intercropped soybean with tall sorghum on competitive ability of soybean and economic yield at Otobi, Benue State, Nigeria
Authors: O. M. Egbe
Journal: Journal of Clinical Oncology (2010)

Field experiment was conducted for three years to evaluate the influence of plant population density of intercropped soybean with sorghum on its competitive ability and economic yield at Otobi, Benue State, Nigeria. The experiment was laid out in split-split plot with three replications. The main plot treatment comprised of cropping systems with two levels (sole cropping, intercropping), while the sub-plot treatment was soybean variety at three levels (TGX 536-O2D, Samsoy 2, TGX 923-2E) and the sub-sub-plot treatment was plant population density of soybean at three levels (200,000 plants/ha; 333,000 plants/ha and 400,000 plants/ha). Planting intercropped soybean at 333,000 plants/ha gave significantly higher seed yield than planting at 400,000 plants/ha, which in turn had greater seed yield than planting at 200,000 plants/ha during the three years of experimentation. Increased density of soybean beyond 333,000 plants/ha in the intercrop reduced sorghum yield. The results showed that all the intercrop combinations had land equivalent ratio (LER) and area- time equivalency ratio( ATER) above unity (1.63 - 1.97) and (1.41 - 1.47),respectively, under all the densities of soybean tested, suggesting a considerable benefit for intercropping soybean with sorghum. LER figures decreased with increase in soybean density. ATER was highest at 333.000 plants/ha, suggesting higher productivity at this density. Aggressivity values were consistently negative at 200,000 plants/ha with a mean value of -0.25 and it was inconsistent at 333,000plants/ha and 400,000 plants/ha. The competitive ratio of soybean increased (0.76 - 1.15) with increasing density of the soybean in the intercrop combinations, indicating higher competitiveness at higher densities than the sorghum component. The competitive ratio of sorghum had the opposite response (1.23 - 0.76). Relative crowding coefficient (K) was inconsistent at all densities, while land equivalent coefficient (LEC) was above 0.25 indicating intercrop advantages for all combinations. ‘Soybean yield equivalent’ was also highest at 333,000 plants/ha (1.70) implying general suitability of soybean to intercropping at this density. Dominance analysis revealed that sole crop treatments were dominated. Intercropped soybean planted at 333,000 plants/ha and 400,000 plants/ha gave significantly higher net benefits than those planted at 200,000 plants/ha. Marginal analysis showed that planting soybean at 333,000 plants/ha gave the highest marginal rate of return and this was 2825%.These results suggest that growing soybean under intercropping at Otobi is biologically efficient at 333,000 plants/ha and more profitable.

	 

	Key words: Intercropped soybean, density, competition, Otobi.
Clinicopathological features of CD56+ nasal-type T/natural killer cell lymphomas with lobular panniculitis
Authors: S-E. Chang, J. Huh, J-H. Choi, K-J. Sung, K-C. Moon, J-K. Koh
Journal: British Journal of Dermatology (2000)

Nasal-type T/natural killer cell lymphoma (TNKCL) shows frequent extranodal involvement including the skin, and is associated with a poor prognosis. We have studied six patients with nasal-type TNKCL presenting with inflammatory subcutaneous nodular lesions with a subcutaneous lymphoid infiltrate. Clinical information was obtained from the medical records of the patients and at follow-up. All cases showed features of angiocentric lymphoma on histology, although there was diffuse cellular infiltration rather than an angiocentric pattern in the subcutis. All six patients were similar in immunophenotype: positive for CD56 and either cytoplasmic CD3 or CD45RO, but negative for B-cell markers and CD30. In situ hybridization using an anti-sense Epstein-Barr virus early regions probe showed a positive reaction in all cases. All patients either died with progressive disease or showed no response to combined chemotherapy. The diagnosis of nasal-type TNKCL, which has a fatal outcome, is facilitated by detection of CD56-positive tumour cells. In evaluating lobular panniculitis including apparently benign inflammatory subcutaneous nodules, nasal-type TNKCL should be considered in the differential diagnosis, especially in Asian countries.
A common polymorphism in KCNH2 (HERG) hastens cardiac repolarization
Authors: C Bezzina
Journal: Cardiovascular Research (2003)

Objective: Genetic variants of cardiac ion channels may influence cardiac repolarization. Thereby such variants may modulate the penetrance of primary electrical disorders, contribute to differences in susceptibility to drug-induced QT-prolongation between individuals, or contribute to rhythm disturbances in the context of structural heart disease. Since the current encoded by KCNH2 (HERG; IKr) is a primary determinant of repolarization, we conducted association studies between the respective alleles of the common amino acid-changing polymorphism at codon 897 (2690A>C; K897T) within HERG and rate-corrected QT interval (QTc). Methods and Results: Association analysis in Caucasian subjects (n = 1030) revealed a significant association of this polymorphism with QTc (P = 0.0025) with CC homozygotes having a significantly shorter QTc (388.5±2.9 ms) compared to AA homozygotes (398.5±0.9) and heterozygotes (AC, 397.2±1.2). The latter two genotypes were associated with comparable mean QTc's, suggesting that the 2690C-allele is recessive. After stratification by sex, the polymorphism was more predictive of QTc in females (P = 0.0021), a finding that was replicated in a second population sample (n = 352) from the same ethnic background (P = 0.044). To assess whether this polymorphism could represent a 'functional' polymorphism, we compared the biophysical properties of K897- and T897-HERG channels by whole-cell voltage clamp. Compared to the K897 channel, the T897 channel displayed a shift of −7 mV in voltage dependence of activation and increased rates of current activation and deactivation. Conclusion: As confirmed in modeling studies, these changes are expected to shorten action potential duration by an increase in IKr. This recapitulates the shorter QTc in females homozygous for the 2690C-allele.
Level of agreement of the 1987 ACR and 2010 ACR/EULAR rheumatoid arthritis classification criteria: an analysis based on ESPOIR cohort data
Authors: B Fautrel, B Combe, N Rincheval, M Dougados
Journal: Annals of the Rheumatic Diseases (2011)

Background In 2010, new classification criteria for rheumatoid arthritis (RA) were developed. Objective To assess agreement between 1987 American College of Rheumatology (ACR) and 2010 ACR/European League Against Rheumatism (EULAR) criteria and the potential source of discordance, based on ESPOIR cohort data. Methods 813 early arthritis patients were included in ESPOIR between 2002 and 2005. Between-criteria agreement was based on the κ coefficient. Discordance was explored by logistic regression. Results Data for 811 patients were available, with their main characteristics as follows: women 77%, swollen joint count 7.2, tender joint count 8.4, disease activity score in 28 joints 5.2, rheumatoid factor 46%, anticitrullinated protein antibody (ACPA) 39%, structural damage 22%. At baseline, 579 (71.4%) patients met the 1987 ACR criteria and 641 (79.0%) the 2010 criteria. Agreement at baseline was discordant for 168 patients: 115 satisfied the 2010 criteria and 53 the 1987 criteria. Concordance between the two sets was fair, with a κ coefficient of 0.45 and 0.42 at baseline and year 2, respectively. The main sources of discordance were the number and symmetry of joint involvement, as well as ACPA status. Conclusion 2010 ACR/EULAR criteria identified more patients with RA than did 1987 criteria. The 2010 criteria failed to identify RA patients with symmetrical seronegative arthritis and limited joint involvement.
Prognostic and predictive value of a microRNA signature in stage II colon cancer: a microRNA expression analysis
Authors: Jia-Xing Zhang, Wu Song, Zhen-Hua Chen, Jin-Huan Wei, Yi-Ji Liao, Jian Lei, Ming Hu, Geng-Zhen Chen, Bing Liao, Jian Lu, Hong-Wei Zhao, Wei Chen, Yu-Long He, Hui-Yun Wang, Dan Xie, Jun-Hang Luo
Journal: The Lancet Oncology (2013)
Fibrinogen γ Overexpression in Pancreatic Cancer Identified by Large-scale Proteomic Analysis of Serum Samples
Authors: Mark Bloomston, Jeff X. Zhou, Alexander S. Rosemurgy, Wendy Frankel, Carlos A. Muro-Cacho, Timothy J. Yeatman
Journal: Cancer Research (2006)

Abstract Detection of serum markers for pancreatic cancer has been elusive. Although CA 19-9 is most commonly used, its sensitivity and specificity are modest. We used large-scale proteomics to identify potential serum markers for pancreatic cancer. Samples were analyzed using high-resolution two-dimensional gel electrophoresis to identify differentially expressed proteins in 32 normal and 30 pancreatic cancer patients. Up to 1,744 protein spots were resolved for each serum sample. Candidate proteins were identified using mass spectrometry. ANOVA was used to identify proteins that could discriminate cancer from normal sera. Serum fibrinogen level was also measured using enzymatic assay. Immunohistochemistry was used to detect fibrinogen in resected pancreatic cancers. One hundred fifty-four proteins were commonly overexpressed in all pancreatic cancers. Nine protein spots (four with identifications by mass spectrometry) could effectively separate cancer from normal controls using cross-validation. These proteins successfully discriminated all pancreatic cancer samples (30 of 30) and 94% of normal (30 of 32) samples. Prominent among these candidates was fibrinogen γ, which was subsequently confirmed to be overexpressed in pancreatic cancer sera by enzymatic analysis (54.1 ± 64.1 versus 0.0 ± 0.0 mg/dL, P &amp;lt; 0.05) and tissue by immunohistochemistry (67% versus 29%, P &amp;lt; 0.05) relative to normal pancreas. Proteomic analysis combining two-dimensional gel electrophoresis and mass spectrometry successfully identified 154 potential serum markers for pancreatic cancer. Of these, fibrinogen γ, a protein associated with the hypercoagulable state of pancreatic cancer, discriminated cancer from normal sera. Fibrinogen is a potential tumor marker in pancreatic cancer. (Cancer Res 2006; 66(5): 2592-9)
Lower-Limb Vascularization in Diabetic Patients
Authors: Emmanuel Cosson, Frédéric Paycha, Philippe Tellier, Régis Nessim Sachs, Arabi Ramadan, Jacques Paries, Jean-Raymond Attali, Paul Valensi
Journal: Diabetes Care (2001)

OBJECTIVE—To investigate, by thallium-201 scanning, circulation in the muscles of the lower limb (LL) in diabetic patients without clinical peripheral vascular disease but with a high cardiovascular risk profile. RESEARCH DESIGN AND METHODS—A total of 80 diabetic patients (76 patients with type 2 diabetes, mean age 57.3 years, duration of diabetes 13.0 ± 7.5 years) with more than one additional cardiovascular risk factor but no claudication were investigated. After stress testing, 1.5 MBq/kg thallium-201 was administered to perform myocardial single-photon emission computed tomography followed by LL scanning. Muscle blood flow was considered abnormal if the asymmetry in thallium-201 uptake between the two buttocks and/or thighs and/or calves was &amp;gt;10%. RESULTS—Muscle perfusion defects were found in 42% of the patients, mainly in the calves. These defects correlated with retinopathy (P = 0.042) and the HbA1c level (P = 0.044). In patients with defects in the buttock and/or thigh, the prevalence of nephropathy and retinopathy was higher than in those with isolated defects in the calf (P = 0.032 and 0.023, respectively). CONCLUSIONS—This study suggests that LL scanning coupled with myocardial scintigraphy is a convenient method of investigating peripheral muscle circulation. Proximal perfusion defects in patients without clinical arterial disease are associated with increased prevalence of retinopathy and nephropathy and, therefore, may be due to microvascular disease of LL muscle. Distal defects may indicate silent macrovascular disease of the LL.
Sorafenib in patients with Child-Pugh class A and B advanced hepatocellular carcinoma: a prospective feasibility analysis
Authors: T. Pressiani, C. Boni, L. Rimassa, R. Labianca, S. Fagiuoli, S. Salvagni, D. Ferrari, E. Cortesi, C. Porta, C. Mucciarini, L. Latini, C. Carnaghi, M. Banzi, S. Fanello, M. De Giorgio, F.R. Lutman, G. Torzilli, M.A. Tommasini, R. Ceriani, G. Covini, M.C. Tronconi, L. Giordano, N. Locopo, S. Naimo, A. Santoro
Journal: Annals of Oncology (2012)