tbuckley/cabot-search huggingface dataset:
A Quantitative Autoradiographic Study of Nucleolus-Associated RNA and DNA Synthesis During the Eclipse Phase in Rous Sarcoma Virus-Infected Chicken Fibroblasts&lt;xref ref-type="fn" rid="fn2"&gt;2&lt;/xref&gt;
Authors: R G Suskind, S Michelson-Fiske, F Haguenau, G F Rabotti
Journal: JNCI Journal of the National Cancer Institute (1975)

Functional and morphologic differences between the sensitivity of nucleoli of Rous sarcoma virus-transformed cells and that of newly infected cells to the action of actinomycin D (AD) have been demonstrated by quantitative light and electron microscope autoradiography and utilized to investigate the function of the nucleolus in the early stages of infection. After a pulse exposure to low doses of AD, increased RNA synthesis is induced within 80 minutes in the fibrillar portion of the nucleolus by infection. A concomitant increase in the retention of tritiated AD in the nucleolus and a quantitative redistribution of intranuclear and cytoplasmic DNA label are interpreted as evidence for a virus-induced amplification of the binding sites of AD in nucleolar chromatin.
Asthma and Rhinitis
Journal: Thorax (1995)

secretions were high enough".Ms Webber, who reviewed the paper when it was first submitted, is entitled to question the ACBT technique described in this study.However, the authors should have followed the statement "at a higher lung volume" by "when secretions were in the upper airways".The physiotherapy during the study was su- pervised throughout by the same physio- therapist who is experienced and has a special interest in cystic fibrosis.The authors are therefore confident that the ACBT method used fulfils all of the characteristics of a sat- isfactory technique.The only independent arbiters of the method used are the patient subjects them- selves.One of the authors (RN) has therefore personally interviewed six of the more mature and easily available subjects who are known to carry out treatment of their fibrosis conscientiously.Each was first informed that a leading authority on the use of physiotherapy in cystic fibrosis had questioned the method ofACBT used and the validity ofour findings, and that we needed to investigate whether the ACBT that they had been taught was correct.Each subject was asked the same non- committal question: "During ACBT, what characteristic must be fulfilled before you change from mid to low volume huffing to high volume huffing?"In each case the answer was when secretions are felt in the large upper airways.All have agreed that their names, addresses, and telephone numbers may be supplied to the editor if required.We are therefore confident that patients under the care of the Newcastle Cystic Fib- rosis Clinic are being taught and are per- forming ACBT correctly, and that the method used in the study was that described in the published version.We are equally confident that ACBT was used correctly during the study, and that it provides a true comparison between ACBT and autogenic drainage.
The Effectiveness Of 30-40 Mm Hg Compression Stockings To Treat Acute Leg Pain Associated With Proximal Deep Vein Thrombosis: Results From The Sox Randomized Controlled Trial
Authors: Susan R. Kahn, Stan Shapiro, Adrielle H Houweling, Thierry Ducruet, Philip S. Wells, Marc A Rodger, Michael J Kovacs, David Anderson, Vicky Tagalakis, Susan Solymoss, Marie-José Miron, Erik Yeo, Reginald Smith, Sam Schulman, Jeannine Kassis, Clive Kearon, Isabelle Chagnon, Turnly Wong, Christine Demers, Rajendar Hanmiah, Scott Kaatz, Rita Selby, Suman Rathbun, Sylvie Desmarais, Lucie Opatrny, Thomas L. Ortel, Jean-Philippe Galanaud, Jeffrey S Ginsberg
Journal: Blood (2013)
Early Changes in Kidney Distribution under the New Allocation System
Authors: Allan B. Massie, Xun Luo, Bonnie E. Lonze, Niraj M. Desai, Adam W. Bingaman, Matthew Cooper, Dorry L. Segev
Journal: Journal of the American Society of Nephrology (2015)

The Kidney Allocation System (KAS), a major change to deceased donor kidney allocation, was implemented in December 2014. Goals of KAS included directing the highest-quality organs to younger/healthier recipients and increasing access to deceased donor kidney transplantation (DDKT) for highly sensitized patients and racial/ethnic minorities. Using national registry data, we compared kidney distribution, DDKT rates for waitlist registrants, and recipient characteristics between January 1, 2013, and December 3, 2014 (pre-KAS) with those between December 4, 2014, and August 31, 2015 (post-KAS). Regional imports increased from 8.8% pre-KAS to 12.5% post-KAS; national imports increased from 12.7% pre-KAS to 19.1% post-KAS ( P &lt;0.001). The proportion of recipients &gt;30 years older than their donor decreased from 19.4% to 15.0% ( P &lt;0.001). The proportion of recipients with calculated panel-reactive antibody =100 increased from 1.0% to 10.3% ( P &lt;0.001). Overall DDKT rate did not change as modeled using exponential regression adjusting for candidate characteristics ( P =0.07). However, DDKT rate (incidence rate ratio, 95% confidence interval) increased for black (1.19; 1.13 to 1.25) and Hispanic (1.13; 1.05 to 1.20) candidates and for candidates aged 18–40 (1.47; 1.38 to 1.57), but declined for candidates aged &gt;50 (0.93; 0.87 to 0.98 for aged 51–60 and 0.90; 0.85 to 0.96 for aged &gt;70). Delayed graft function in transplant recipients increased from 24.8% pre-KAS to 29.9% post-KAS ( P &lt;0.001). Thus, in the first 9 months under KAS, access to DDKT improved for minorities, younger candidates, and highly sensitized patients, but declined for older candidates. Delayed graft function increased substantially, possibly suggesting poorer long-term outcomes.
Fludarabine, cyclophosphamide, and rituximab treatment achieves long-term disease-free survival in IGHV-mutated chronic lymphocytic leukemia
Authors: Philip A. Thompson, Constantine S. Tam, Susan M. O’Brien, William G. Wierda, Francesco Stingo, William Plunkett, Susan C. Smith, Hagop M. Kantarjian, Emil J. Freireich, Michael J. Keating
Journal: Blood (2015)
Thailandepsin A-loaded and octreotide-functionalized unimolecular micelles for targeted neuroendocrine cancer therapy
Authors: Renata Jaskula-Sztul, Wenjin Xu, Guojun Chen, April Harrison, Ajitha Dammalapati, Renu Nair, Yiqiang Cheng, Shaoqin Gong, Herbert Chen
Journal: Biomaterials (2016)
Activity and safety of ceritinib in patients with ALK-rearranged non-small-cell lung cancer (ASCEND-1): updated results from the multicentre, open-label, phase 1 trial
Authors: Dong-Wan Kim, Ranee Mehra, Daniel S W Tan, Enriqueta Felip, Laura Q M Chow, D Ross Camidge, Johan Vansteenkiste, Sunil Sharma, Tommaso De Pas, Gregory J Riely, Benjamin J Solomon, Jürgen Wolf, Michael Thomas, Martin Schuler, Geoffrey Liu, Armando Santoro, Santosh Sutradhar, Siyu Li, Tomasz Szczudlo, Alejandro Yovine, Alice T Shaw
Journal: The Lancet Oncology (2016)
PWE-074 Faecal calprotectin in predicting relapse in inflammatory bowel disease: a meta-analysis
Authors: N Mohammed, E Telakis, V Subramanian
Journal: Gut (2015)

<h3>Introduction</h3> Faecal calprotectin (FCP) is a non-invasive marker of gastrointestinal inflammation. Its utility in the clinical management of inflammatory bowel disease (IBD) is under evaluation. We aimed to perform a meta-analysis of prospective studies in assessing the ability of baseline FCP for predicting relapse with 12 months in patients with Crohn’s disease (CD) and ulcerative colitis (UC) in clinical remission. <h3>Method</h3> Multiple electronic databases were searched including Pubmed, Embase and Ovid looking for studies providing data on relapse prediction in IBD using FCP. Pooled sensitivity, specificity, negative (LR-) and positive (LR=+ predictive value diagnostic odds ratio (DOR) and pooled area under the receiver operating characteristic (AUROC) was calculated. A random effects model was used and publication bias was assessed using Funnel plots and Egger’s test and heterogeneity was assessed using Cochran’s Q and the I<sup>2</sup>test. <h3>Results</h3> 8 studies involving 507 patients with CD and 8 studies involving 587 patients with UC were included. The predictive value for a relapse within 12 months for baseline FCP in patients with CD was sensitivity 73% (64–80), specificity 78% (74–82), LR+ 2.9 (1.9–4.5), LR- 0.4 (0.2–0.6), DOR 10.1 (4.5–22.6) and AUROC 0.83 (±0.04). The cut off for baseline FCP used for the CD studies ranged from 130–340 µg/g. The predictive value for a relapse within 12 months for baseline FCP in patients with UC was sensitivity 72% (65–77), specificity 78% (74–83), LR+ 3.0 (2.3–4.0), LR- 0.4 (0.2–0.6), DOR 9.2 (5.4–15.7) and AUROC 0.82 (±0.03). The cut off for baseline FCP used for the UC studies ranged from 130–250 µg/g. There was significant heterogeneity (I<sup>2</sup>&gt; 50%) for all the analysis likely because of the differences in relapse rates and FCP cut off values used. <h3>Conclusion</h3> FCP is a simple non-invasive marker with the potential to predict replase in IBD. With pooled sensitivity and specificity under 80% for both CD and UC, its value may be mainly to identify low risk patients. However wide variations in the cut off values for FCP used in these studies makes it difficult to apply this to routine clinical practice. Serial measurements of FCP to check for a rise from baseline may be the way forward for future studies. <h3>Disclosure of interest</h3> None Declared.
Increase in Reported Prevalence of Microcephaly in Infants Born to Women Living in Areas with Confirmed Zika Virus Transmission During the First Trimester of Pregnancy — Brazil, 2015
Authors: Wanderson Kleber de Oliveira, Juan Cortez-Escalante, Wanessa Tenório Gonçalves Holanda De Oliveira, Greice Madeleine Ikeda do Carmo, Cláudio Maierovitch Pessanha Henriques, Giovanini Evelim Coelho, Giovanny Vinícius Araújo de França
Journal: MMWR Morbidity and Mortality Weekly Report (2016)

Widespread transmission of Zika virus by Aedes mosquitoes has been recognized in Brazil since late 2014, and in October 2015, an increase in the number of reported cases of microcephaly was reported to the Brazil Ministry of Health.* By January 2016, a total of 3,530 suspected microcephaly cases had been reported, many of which occurred in infants born to women who lived in or had visited areas where Zika virus transmission was occurring. Microcephaly surveillance was enhanced in late 2015 by implementing a more sensitive case definition. Based on the peak number of reported cases of microcephaly, and assuming an average estimated pregnancy duration of 38 weeks in Brazil (1), the first trimester of pregnancy coincided with reports of cases of febrile rash illness compatible with Zika virus disease in pregnant women in Bahia, Paraíba, and Pernambuco states, supporting an association between Zika virus infection during early pregnancy and the occurrence of microcephaly. Pregnant women in areas where Zika virus transmission is occurring should take steps to avoid mosquito bites. Additional studies are needed to further elucidate the relationship between Zika virus infection in pregnancy and microcephaly.
Adoptive Cell Therapy with Tumor Infiltrating Lymphocytes and Intermediate Dose Interleukin-2 for Metastatic Melanoma
Authors: R. Andersen, M. Donia, E. Ellebaek Steensgaard, T. Holz Borch, T. Zeeberg Iversen, P. Kongsted, M. Hald Andersen, P. Thor Straten, I.M. Svane
Journal: Annals of Oncology (2014)
Effects of the BET-inhibitor, RVX-208 on the HDL lipidome and glucose metabolism in individuals with prediabetes: A randomized controlled trial
Authors: Andrew L. Siebel, Si Khiang Trinh, Melissa F. Formosa, Piyushkumar A. Mundra, Alaina K. Natoli, Medini Reddy-luthmoodoo, Kevin Huynh, Anmar A. Khan, Andrew L. Carey, Gerrit van Hall, Claudio Cobelli, Chiara Dalla-Man, Jim D. Otvos, Kerry-Anne Rye, Jan Johansson, Allan Gordon, Norman C.W. Wong, Dmitri Sviridov, Philip Barter, Stephen J. Duffy, Peter J. Meikle, Bronwyn A. Kingwell
Journal: Metabolism (2016)
Toxin Immunoassays and<i>Clostridium difficile</i>Infection
Authors: Ferric C. Fang
Journal: JAMA Internal Medicine (2016)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
An increased prevalence of self‐reported allergic rhinitis in major Chinese cities from 2005 to 2011
Authors: X. D. Wang, M. Zheng, H. F. Lou, C. S. Wang, Y. Zhang, M. Y. Bo, S. Q. Ge, N. Zhang, L. Zhang, C. Bachert
Journal: Allergy (2016)

The prevalence of allergic rhinitis (AR) has increased worldwide in recent decades. This study was conducted to investigate the prevalence of self-reported AR and profiles of AR-related comorbidities in the adult population of China over time.
Abstract 5804: Embryonic Stem Cell-Derived Endothelial Cells Engraft Into the Ischemic Hindlimb and Restore Perfusion
Authors: Ngan F Huang, Hiroshi Niiyama, Christoph Peter, Abhiiit De, Yasodha Natkunam, Felix Fleissner, Zongjin Li, Mark D Rollins, Joseph C Wu, Sanjiv S Gambhir, John P Cooke
Journal: Circulation (2009)

Peripheral arterial disease (PAD), which affects about 10 million Americans, is typically due to atherosclerotic arterial occlusive disease of the limbs and is associated with dysfunctional vascular endothelium. Cell-based therapy using endothelial cells derived from embryonic stem cells (ESC-ECs) is a promising approach for regenerating the endothelium. However, studies that characterize the localization and engraftment of embryonic stem cells (ESCs) and ESC-ECs into the ischemic limb are limited. In this study, we examined the survival, localization, and functional effects of exogenously administered ESC-ECs for repair of hindlimb ischemia, which is a murine model of PAD. Murine ESCs or ESC-ECs were stably transduced with a construct for bioluminescence imaging (BLI) and fluorescent detection. In a syngenic murine model of hindlimb ischemia, ESCs or ESC-ECs were delivered by intramuscular (IM), intra-femoral artery (IA), or intra-femoral vein (IV) injections (n=5 in each group). For 2 weeks, cell survival and localization were tracked by BLI and confirmed by gene expression, and functional improvement was assessed by laser Doppler blood perfusion. BLI results showed ESC persisting in the ischemic limb after IM or IA, but not after IV administration. Regardless of the route of administration, ESCs were detected outside of the hindlimb circulation in the spleen or lungs. ESCs did not improve limb perfusion, and induced teratomas. In contrast, ESC-ECs localized to the ischemic limb in all cases as assessed by BLI. Immunohistochemical studies confirmed the engraftment of ESC-ECs into the limb vasculature after 2 weeks. Intriguingly, ESC-ECs were not detected in the spleen or lungs after 2 weeks, even after IV administration. Furthermore, ESC-ECs improved limb perfusion. In comparison to parental ESCs, ESC-ECs preferentially localized in the ischemic hindlimb, even after IV delivery. ESC-EC localization was temporally related to the improvement in hindlimb perfusion, which was associated with engraftment of ESC-ECs into the ischemic microvasculature. This research has received full or partial funding support from the American Heart Association, Western States Affiliate (California, Nevada &amp; Utah).
Sortilin mediates vascular calcification via its recruitment into extracellular vesicles
Authors: Claudia Goettsch, Joshua D. Hutcheson, Masanori Aikawa, Hiroshi Iwata, Tan Pham, Anders Nykjaer, Mads Kjolby, Maximillian Rogers, Thomas Michel, Manabu Shibasaki, Sumihiko Hagita, Rafael Kramann, Daniel J. Rader, Peter Libby, Sasha A. Singh, Elena Aikawa
Journal: Journal of Clinical Investigation (2016)

Vascular calcification is a common feature of major cardiovascular diseases. Extracellular vesicles participate in the formation of microcalcifications that are implicated in atherosclerotic plaque rupture; however, the mechanisms that regulate formation of calcifying extracellular vesicles remain obscure. Here, we have demonstrated that sortilin is a key regulator of smooth muscle cell (SMC) calcification via its recruitment to extracellular vesicles. Sortilin localized to calcifying vessels in human and mouse atheromata and participated in formation of microcalcifications in SMC culture. Sortilin regulated the loading of the calcification protein tissue nonspecific alkaline phosphatase (TNAP) into extracellular vesicles, thereby conferring its calcification potential. Furthermore, SMC calcification required Rab11-dependent trafficking and FAM20C/casein kinase 2-dependent C-terminal phosphorylation of sortilin. In a murine model, Sort1-deficiency reduced arterial calcification but did not affect bone mineralization. Additionally, transfer of sortilin-deficient BM cells to irradiated atherosclerotic mice did not affect vascular calcification, indicating a primary role of SMC-derived sortilin. Together, the results of this study identify sortilin phosphorylation as a potential therapeutic target for ectopic calcification/microcalcification and may clarify the mechanism that underlies the genetic association between the SORT1 gene locus and coronary artery calcification.
AMPK Promotes Autophagy by Facilitating Mitochondrial Fission
Authors: Chen-Song Zhang, Sheng-Cai Lin
Journal: Cell Metabolism (2016)

AMPK senses decreased cellular energy levels, triggering mitochondrial autophagy or mitophagy through activating ULK1 and inhibiting mTORC1. In a recent report, Toyama et al. (2016) show that activated AMPK phosphorylation of MFF is critical for mitochondrial fission. Unphosphorylatable MFF mutants block mitophagy, connecting AMPK to mitochondrial fission and to mitophagy.
Sharing decision-making between patient and clinician: the next step in evidence-based practice for patellofemoral pain?
Authors: C J Barton, K M Crossley
Journal: British Journal of Sports Medicine (2016)

The ‘Best Practice Guide to Conservative Management of Patellofemoral Pain’1 synthesised level 1 evidence from high-quality systematic reviews with international expert opinion. The associated publication and guide (see table 1) provides a tool to facilitate research translation and evidence-based practice (EBP) in the management of patellofemoral pain (PFP). This tool is freely available to clinicians treating PFP worldwide.

View this table:

Table 1 
Best Practice Guide to Conservative Management of Patellofemoral Pain—adapted from Barton et al .1 



The ‘Best Practice Guide’ outlines available evidence for various passive, active and educational interventions. However, it makes up just one of the three key components of EBP. By definition, EBP incorporates (1) the best external evidence, (2) the clinician's individual experience, and (3) the patient's beliefs and expectations.2 Therefore, the published guide alone does not capture the clinicians experience related to the perception of how this evidence might apply to …
Prognostic relevance of lactate dehydrogenase and serum S100 levels in stage IV melanoma with known<i>BRAF</i>mutation status
Authors: A.L. Frauchiger, J. Mangana, M. Rechsteiner, H. Moch, B. Seifert, R.P. Braun, R. Dummer, S.M. Goldinger
Journal: British Journal of Dermatology (2015)

Activating mutations of BRAF provide an important treatment target in patients with melanoma. The prognostic role of several biochemical markers in relation to mutation status is not clear.To analyse the prognostic significance of BRAF mutation in patients with melanoma and correlate it to different markers.In total, 162 patients with stage IV melanoma and known BRAF mutation status were included. Clinical, histopathological and laboratory information was collected and compared between patients with BRAF mutant (BRAFm) and wild-type (BRAFwt) melanoma at the time of first distant metastasis.In total, 88 patients (54%) had BRAFm melanoma (V600E/V600K). At the first distant metastasis, S100B levels in BRAFm patients were more frequently elevated (P = 0·01) and significantly higher (P = 0·02). Median overall survival (mOS) was significantly longer in BRAFwt patients with normal compared with patients with elevated S100B levels (P < 0·01). In BRAFm melanoma, elevated S100B levels showed no prognostic influence (P = 0·18). Elevated lactate dehydrogenase (LDH) levels had a significantly negative impact on mOS in both groups. mOS was increased for BRAFm patients treated with a BRAF inhibitor (BRAFi) compared with BRAFm patients not receiving BRAFi (P = 0·01). No difference in mOS between BRAFm patients who did not receive BRAFi treatment and BRAFwt patients was observed.Better mOS was observed in BRAFm patients treated with BRAFi. BRAFm patients not treated with BRAFi show similar survival curves to BRAFwt patients. Elevated LDH is a BRAF-independent prognostic parameter; S100B has prognostic significance in BRAFwt melanoma only.
Oral contraception and liver damage. (Letter to the editor.)
Authors: Tyler Et
Journal: BMJ (1964)

Comments are made on correspondence reporting liver toxicity resulting from oral contraceptive pills.  A. Eisalo and his colleagues reported 7 patients who developed hepatic toxicity while taking Lyndiol and I.P. Palva and O.O. Mustala related their findings concerning 5 consecutive postmenopausal patients who demonstrated hepatic toxicity on  Anovlar.  The writer states that no similar occurrence of abnormal liver function tests took place in studies of hundreds of patients.  He believes that it is rash to conclude that there is an association between oral contraception and liver damage on the basis of 2 small studies but does agree that further studies are indicated.
Gene regulatory mechanisms underpinning prostate cancer susceptibility
Authors: Thomas Whitington, Ping Gao, Wei Song, Helen Ross-Adams, Alastair D Lamb, Yuehong Yang, Ilaria Svezia, Daniel Klevebring, Ian G Mills, Robert Karlsson, Silvia Halim, Mark J Dunning, Lars Egevad, Anne Y Warren, David E Neal, Henrik Grönberg, Johan Lindberg, Gong-Hong Wei, Fredrik Wiklund
Journal: Nature Genetics (2016)