tbuckley/cabot-search huggingface dataset:
Elevated proto-oncogene expression in polycystic kidneys of the C57BL/6J (cpk) mouse.
Authors: B D Cowley, L J Chadwick, J J Grantham, J P Calvet
Journal: Journal of the American Society of Nephrology (1991)

Polycystic kidney disease in the C57BL/6J (cpk) mouse is an autosomal recessive disorder which leads to the rapid development of renal cysts and kidney failure during the first 3 to 4 postnatal weeks. Previously, we showed that the cystic kidneys of affected mice have abnormally elevated levels of c-myc mRNA. In the study presented here, it is shown that mRNAs for the proto-oncogenes c-fos and c-Kiras, as well as c-myc, are markedly elevated in cystic kidneys, suggesting that there is a more general abnormality in gene expression associated with the disease. It is also evident that there are two stages to this abnormal proto-oncogene expression. In the first stage, which occurs up through the second postnatal week, there are modest increases in proto-oncogene mRNA which parallel the increased cell proliferation that accompanies cyst growth at this time. In the second stage, which occurs after the second postnatal week, there are markedly elevated levels of proto-oncogene mRNA that are seen at a time when cell proliferation is declining. The development of this latter stage suggests either that there is a fundamental abnormality intrinsic to polycystic kidneys that leads to uncontrolled proto-oncogene expression later in disease progression or that there is a secondary response in the kidney to the progressive renal failure.
Annual Outcomes With Transcatheter Valve Therapy
Authors: David R. Holmes, Rick A. Nishimura, Frederick L. Grover, Ralph G. Brindis, John D. Carroll, Fred H. Edwards, Eric D. Peterson, John S. Rumsfeld, David M. Shahian, Vinod H. Thourani, E. Murat Tuzcu, Sreekanth Vemulapalli, Kathleen Hewitt, Joan Michaels, Susan Fitzgerald, Michael J. Mack
Journal: Journal of the American College of Cardiology (2015)
Phase II trial of bimonthly leucovorin, 5-fluorouracil and gemcitabine for advanced pancreatic adenocarcinoma (FOLFUGEM)
Authors: C. Louvet, T. André, P. Hammel, F. Selle, B. Landi, S. Cattan, M. Fonck, M. Flesch, P. Colin, J. Balosso, P. Ruszniewski, A. de Gramont
Journal: Annals of Oncology (2001)
Doxorubicin-Cardiotoxicity is Blunted If Ranolazine is Administered After Doxorubicin Treatment, in Experimental Models
Authors: R.V. Iaffaioli, C. Coppola, G. Piscopo, D. Rea, F. Galletta, C. Maurea, C. Arra, N. Maurea
Journal: Annals of Oncology (2014)
Effect of Glucose on Intercellular Junctions of Cultured Human Peritoneal Mesothelial Cells
Authors: TAKAFUMI ITO, NORIAKI YORIOKA, MASAO YAMAMOTO, KATSUKO KATAOKA, MICHIO YAMAKIDO
Journal: Journal of the American Society of Nephrology (2000)

Abstract. During continuous ambulatory peritoneal dialysis, the peritoneum is directly and continuously exposed to unphysiologic peritoneal dialysis fluid; the resulting mesothelial damage has been suggested to cause loss of ultrafiltration and dialysis efficacy. The present study investigated the effect of a high glucose concentration on cultured human peritoneal mesothelial cells to clarify the cause of decreased dialysis efficacy during prolonged peritoneal dialysis. High glucose caused a concentration-dependent decrease in cell proliferation, damage to the intercellular junctions, and excess production of transforming growth factor-β (TGF-β). The levels of intercellular junctional proteins (ZO-1, E-cadherin, and β-catenin) were decreased, and immuno-staining by anti—ZO-1 and anti— β-catenin antibodies became weaker and often discontinuous along the cell contour. Mannitol had similar but weaker effects at the same osmolality, and an anti—TGF-β neutralizing antibody reduced the effects of high glucose. Therefore, these effects were induced not only by glucose itself but also by hyperosmolality and by a glucose-induced increase of TGF-β. These findings suggest that the peritoneal mesothelium is damaged by prolonged peritoneal dialysis using high glucose dialysate and that impairment of the intercellular junctions of peritoneal mesothelial cells by high glucose dialysate induces peritoneal hyperpermeability and a progressive reduction in dialysis efficacy.
Nonbiocompatible hemodialysis membranes induce apoptosis in mononuclear cells
Authors: J Carracedo, R Ramírez, A Martin-Malo, M Rodríguez, P Aljama
Journal: Journal of the American Society of Nephrology (1998)

Mononuclear cells are activated during hemodialysis. In this study, we provide evidence that in vitro culture of mononuclear cells with Cuprophan, a nonbiocompatible hemodialysis membrane, increases the levels of protein phosphorylation in these cells as well as the expression of surface activation molecules. By contrast, culturing of mononuclear cells with AN69, a more biocompatible membrane, did not increase protein phosphorylation levels or expression of surface activation molecules in these cells. In addition, Cuprophan, but not the AN69 membrane, increased the percentage of mononuclear cell death by apoptosis. Inhibition of G-protein-mediated signal transduction decreased the apoptosis of cells cultured with the Cuprophan membrane. The GTP-binding protein involved in Cuprophan-induced apoptosis was sensitive to the ADP-ribosylating pertussis toxin (PTX). The inhibition of GTP-binding protein decreased apoptosis in the early stage of the activation-induced apoptosis, suggesting that G-proteins are implicated in the transmission of apoptosis-inducing signal(s) but do not interfere with the effector signals that mediate the late stages of apoptotic catabolism. Finally, PTX was capable of inhibiting apoptosis without affecting expression of activation molecules; thus, the inhibition of apoptosis by Cuprophan was not due to quenching of the stimulation signals, because monocytes were still able to be activated by Cuprophan despite the action of PTX. The results obtained in this study suggest that cell activation and apoptosis may be mediated by separate intracellular signals.
Expanding the role of blood progenitor cells
Authors: R Pettengell
Journal: Annals of Oncology (1995)

Five years ago the haemopoietic growth factors were introduced to clinical practice with the aim of reducing the depth and duration of chemotherapy induced neutropenia. Now, they have a wider remit, with important roles in supporting dose intensive treatments and mobilising BPC. Similarly, BPC themselves have until now been predominantly used in autologous transplantation following myeloablative treatments. In the next five years we can expect to see BPC from novel sources manipulated to feature in many new roles, including allogeneic transplantation, multicyclic dose-intensive chemotherapy and gene therapy.
The allelic isozymes of hexose-6-phosphate dehydrogenase isolated from Fundulus heteroclitus: physical characteristics and kinetic properties.
Authors: I J Ropson, D A Powers
Journal: Molecular Biology and Evolution (1989)

Hexose-6-phosphate dehydrogenase (H6PDH-A2; beta-D-glucose:NAD(P)+ oxido-reductase; E.C. 1.1.1.47) of the teleost Fundulus heteroclitus (L.) shows clinal allelic variation along the east coast of North America. Three of the major allelic isozymes have been purified and compared for native molecular weight, subunit molecular weight, isoelectric point, thermal stability, and steady-state kinetic properties (pH 8.0 and 25 degrees C). Significant differences were found among the allelic isozymes for isoelectric point, thermal stability, and some kinetic parameters. The predominant allelic isozyme in northern populations (H6PDH-AcAc) was found to be more sensitive to heat denaturation than were the predominant homozygous allelic isozymes isolated from southern populations (H6PDH-AaAa and H6PDH-AbAb). The H6PDH-AcAc allelic isozyme had both a significantly greater Km for glucose-6-phosphate than did either of the southern phenotypes and a significantly greater Km for NADP+ and Ki of NAD+ than did one of the southern phenotypes (H6PDH-AaAa). While the allelic isozymes are functionally nonequivalent, it is not yet known whether these differences are reflected at higher levels of biological organization.
Celebrating the ACP Centennial: From the <i>Annals</i> Archives—Nurse Practitioners and Physicians
Authors: Deborah Cotton
Journal: Annals of Internal Medicine (2015)

Editorials1 December 2015Celebrating the ACP Centennial: From the Annals Archives—Nurse Practitioners and PhysiciansDeborah Cotton, MD, MPH, Deputy EditorDeborah Cotton, MD, MPH, Deputy EditorSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/M15-2094 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail During the 2015 centennial year of the American College of Physicians, publisher of Annals of Internal Medicine, Annals editors will link an article in each issue with an earlier paper from our archives addressing the same disease or clinical question. We hope our readers find this interesting and we welcome comments, especially from physicians who were practicing at the time of publication of the original paper who might offer insight into the impact of the earlier paper on their knowledge or practice.Among the conclusions of the American College of Physicians position paper on retail health clinics, published in this ...References1. Daniel H, Erickson S; Medical Practice and Quality Committee of the American College of Physicians. Retail health clinics: a policy position paper from the American College of Physicians. Ann Intern Med. 2015;163:869-70. doi:10.7326/M15-0571 LinkGoogle Scholar2. Bates B. Physician and nurse practitioner: conflict and reward. Ann Intern Med. 1975;82:702-6. [PMID: 1137265]. doi:10.7326/0003-4819-82-5-702 LinkGoogle Scholar3. U.S. National Library of Medicine. Changing the Face of Medicine. Dr. Barbara Bates. Bethesda, MD: U.S. National Library of Medicine. Accessed at www.nlm.nih.gov/changingthefaceofmedicine/physicians/biography_25.html on 30 September 2015. Google Scholar Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 December 2015Volume 163, Issue 11Page: 879KeywordsArchivesHospitalsMedicareNursesPatientsTouch ePublished: 1 December 2015 Issue Published: 1 December 2015 Copyright & PermissionsCopyright © 2015 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Response Rates and Outcomes with Enzalutamide for Patients with Metastatic Castration Resistant Prostate Cancer and Visceral Disease in the Prevail Trial
Authors: C. Higano, J. Alumkal, S. Chowdhury, Y. Loriot, C.N. Sternberg, J.S. de Bono, B. Tombal, J. Carles, F.G. Perabo, D. Forer, S. Noonberg, H. Mansbach, T. Beer
Journal: Annals of Oncology (2014)
August 2002 Fellowships Grants and Awards
Journal: Environmental Health Perspectives (2002)

Research on Ethical Issues in Human StudiesNIH invites research grant applications to investigate ethical issues in research using human subjects.The Code of Federal Regulations (45 CFR, Part 46) provides a regulatory framework that all NIH-supported researchers must follow.However, recent developments in biomedical and behavioral researchincluding the rapid growth of new interventions and technologies (e.g., stem cells, genetics research), increasing involvement of foreign populations in clinical research, and concerns about financial conflicts of interest among researchers-challenge investigators' abilities to interpret and apply the regulations.Other situations (e.g., research with vulnerable populations, the use of data banks or archives, research on stigmatizing diseases or conditions) may present difficulties for identifying strategies, procedures, and/or techniques that will enhance and ensure the ethical involvement of human participants in research.The purpose of this PA is to support empirical research addressing the ethical challenges of involving human participants in research in order to inform and optimize protections for human participation in research.Examples of the types of topics that would be appropriate for applications submitted under this announcement include, but are not limited to, the following:Minimizing Risks in Human Research 1) Assess how features of the research and research setting affect evaluations of risks versus potential benefits of different types of research for investigators, institutional review board (IRB) members, and potential participants, groups, and communities.Examples of features of the research or research setting may include characteristics of the participants (e.g., age, health status and stage of disease, ethnic/cultural background, cognitive capacity, social status, gender, incarceration), aspects of the condition/disease (e.g., prevalence, severity, chronicity, degree of disability), and the nation or culture in which the study will take place.2) Identify potential social, psychological, and/or economic harms (e.g., stigma, discrimination, personal distress, loss of insurance coverage, loss of employment) that may be associated with recruitment, participation, or self-determined or study-determined withdrawal from research.Evaluate strategies or procedures for minimizing these harms in regard to individuals', groups', communities', and populations' willingness to participate in different types of research.3) Assess the conditions and assumptions under which IRB evaluation of risk versus potential benefits is similar to or different from the evaluation of risks versus potential benefits by individuals, groups, communities, and populations.
Use of Chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of Entamoeba histolytica adhesion.
Authors: E Li, A Becker, S L Stanley
Journal: The Journal of Experimental Medicine (1988)

We compared the adherence of E. histolytica trophozoites with a panel of lectin-resistant CHO mutants with altered glycosylation patterns. Our results coupled with data from saccharide inhibition studies indicate that terminal N-acetyllactosamine units on Asn-linked complex type oligosaccharides provide the major determinants on the cellular receptor for E. histolytica adhesion.
How to define an area where transmission of arthropod-borne disease is occurring?
Authors: D Domanovic, J Giesecke
Journal: Eurosurveillance (2012)

Preview this article: How to define an area where transmission of arthropod-borne disease is occurring?, Page 1 of 1 < Previous page | Next page > /docserver/preview/fulltext/eurosurveillance/17/20/art20171-en-1.gif
FAP-specific re-directed T cells first in-man study in malignant pleural mesothelioma: experience of the first patient treated
Authors: Magdalena Pircher, Petra Schuberth, Pratiksha Gulati, Simon Sulser, Walter Weder, Alessandra Curioni, Christoph Renner, Ulf Petrausch
Journal: Journal for ImmunoTherapy of Cancer (2015)

Meeting abstracts Median survival of advanced malignant pleural mesothelioma (MPM) is less than 2 years, and novel treatments are urgently needed. Immunotherapy with adoptive T cell transfer is an attractive approach that could be added to current therapeutic concepts. Fibroblast activation protein
Outcome of psychotherapy among early adolescents after trauma
Authors: A K Goenjian, I Karayan, R S Pynoos, D Minassian, L M Najarian, A M Steinberg, L A Fairbanks
Journal: American Journal of Psychiatry (1997)

The authors evaluated the effectiveness of brief trauma/grief-focused psychotherapy among early adolescents exposed to the 1988 earthquake in Armenia.Posttraumatic stress and depressive reactions among treated and not treated subjects were evaluated pre- and postintervention, at 1 1/2 and 3 years after the earthquake, respectively.Severity of posttraumatic stress symptoms significantly decreased among the subjects given psychotherapy, while severity of these symptoms increased significantly among the subjects not treated with psychotherapy. The improvement in posttraumatic stress symptoms was attributable to improvement in all three symptom categories (intrusion, avoidance, and arousal) of posttraumatic stress disorder (PTSD). There was no change in severity of depressive symptoms among subjects given psychotherapy. However, depressive symptoms among subjects not treated with psychotherapy significantly worsened over time. The changes in severity of posttraumatic stress and depressive symptoms were positively correlated within both groups.The findings demonstrate the efficacy of trauma/grief-focused brief psychotherapy in alleviating PTSD symptoms and preventing the worsening of comorbid depression among early adolescents after a catastrophic disaster. The results support the broad use of such school-based interventions after major disasters and demonstrate the cross-cultural applicability of Western psychotherapeutic approaches.
Course of Renal Injury in the Mpv17-Deficient Transgenic Mouse
Authors: THOMAS O'BRYAN, HANS WEIHER, HELMUT G. RENNKE, STEFAN KREN, THOMAS H. HOSTETTER
Journal: Journal of the American Society of Nephrology (2000)

The mutant Mpv17 mouse is a transgenic strain that fails to express a protein that is normally expressed in the kidney and that is associated with peroxisomes. The present studies provide a quantitative examination of renal function and structure in this strain compared to its control CFW strain. By 52 wk of age, the mutant strain developed proteinuria (urinary protein to creatinine ratio: 25 +/- 14 versus 3 +/- 1, mutant versus control), albuminuria (urinary albumin to creatinine ratio: 23 +/- 15 versus 0.1 +/- 0.1, mutant versus control), and hypoalbuminemia (2.1 +/- 0.4 versus 2.5 +/- 0.2 G/dl, mutant versus control), but without arterial hypertension or major reduction in filtration (serum creatinine 0.14 +/- 0.04 versus 0.18 +/- 0.12 mg/dl, mutant versus control). The Mpv17 glomeruli were enlarged (0.98 +/- 0.12 versus 0.52 +/- 0.02 micrometer(3) x 10(6), mutant versus control). Glomerular sclerosis became widespread (95 +/- 3 versus 23 +/- 32%, mutant versus control) and was preceded by mesangiolysis and microaneurysms. Tubulointerstitial disease was conspicuous by its absence. The intrarenal vasculature was normal in the mutant mice. Electron microscopy demonstrated focal foot process fusion and mesangiolysis. Thus, this mutant strain of mouse develops proteinuria and a distinct glomerulopathy including mesangiolysis but little interstitial injury all due to the loss of expression of a single gene.
Lessons from Toxicology: Developing a 21st-Century Paradigm for Medical Research
Authors: Gill Langley, Christopher P. Austin, Anil K. Balapure, Linda S. Birnbaum, John R. Bucher, Julia Fentem, Suzanne C. Fitzpatrick, John R. Fowle, Robert J. Kavlock, Hiroaki Kitano, Brett A. Lidbury, Alysson R. Muotri, Shuang-Qing Peng, Dmitry Sakharov, Troy Seidle, Thales Trez, Alexander Tonevitsky, Anja van de Stolpe, Maurice Whelan, Catherine Willett
Journal: Environmental Health Perspectives (2015)

Biomedical developments in the 21st century provide an unprecedented opportunity to gain a dynamic systems-level and human-specific understanding of the causes and pathophysiologies of disease. This understanding is a vital need, in view of continuing failures in health research, drug discovery, and clinical translation. The full potential of advanced approaches may not be achieved within a 20th-century conceptual framework dominated by animal models. Novel technologies are being integrated into environmental health research and are also applicable to disease research, but these advances need a new medical research and drug discovery paradigm to gain maximal benefits. We suggest a new conceptual framework that repurposes the 21st-century transition underway in toxicology. Human disease should be conceived as resulting from integrated extrinsic and intrinsic causes, with research focused on modern human-specific models to understand disease pathways at multiple biological levels that are analogous to adverse outcome pathways in toxicology. Systems biology tools should be used to integrate and interpret data about disease causation and pathophysiology. Such an approach promises progress in overcoming the current roadblocks to understanding human disease and successful drug discovery and translation. A discourse should begin now to identify and consider the many challenges and questions that need to be solved.
ACCF/ACR/SCCT/SCMR/ASNC/NASCI/SCAI/SIR 2006 Appropriateness Criteria for Cardiac Computed Tomography and Cardiac Magnetic Resonance Imaging⁎⁎Developed in accordance with the principles and methodology outlined by ACCF: Patel MR, Spertus JA, Brindis RG, Hendel RC, Douglas PS, Peterson ED, Wolk MJ, Allen JM, Raskin IE. ACCF proposed method for evaluating the appropriateness of cardiovascular imaging. J Am Coll Cardiol 2005;46:1606–13.
Authors: Robert C. Hendel, Manesh R. Patel, Christopher M. Kramer, Michael Poon, Robert C. Hendel, James C. Carr, Nancy A. Gerstad, Linda D. Gillam, John McB. Hodgson, Raymond J. Kim, Christopher M. Kramer, John R. Lesser, Edward T. Martin, Joseph V. Messer, Rita F. Redberg, Geoffrey D. Rubin, John S. Rumsfeld, Allen J. Taylor, Wm. Guy Weigold, Pamela K. Woodard, Ralph G. Brindis, Robert C. Hendel, Pamela S. Douglas, Eric D. Peterson, Michael J. Wolk, Joseph M. Allen, Manesh R. Patel
Journal: Journal of the American College of Cardiology (2006)
Biocompatibility of a fish scale-derived artificial cornea: Cytotoxicity, cellular adhesion and phenotype, and in vivo immunogenicity
Authors: T.H. van Essen, L. van Zijl, T. Possemiers, A.A. Mulder, S.J. Zwart, C.-H. Chou, C.C. Lin, H.J. Lai, G.P.M. Luyten, M.J. Tassignon, N. Zakaria, A. El Ghalbzouri, M.J. Jager
Journal: Biomaterials (2015)
Safety Evaluation Of Polymer Materials
Authors: T. Darby
Journal: The Annual Review of Pharmacology and Toxicology (1987)

Polymer materials have made important contributions to human well-being during the last fifty years. The different chemical formulas and syntheses of polymers supply an enormous number of materials of widely differing come position. Continuous research and development in the health industry have led to an increased life span and improved quality of life for many patients by providing improved materials for medical devices. Both elastomers and plas­ tics have been devised to 'furnish materials with every conceivable type of physical response. Yet, ideas for new and better materials to replace existing materials are proposed each day. As with other products, safety evaluation of polymer materials and the devices made from them begins with concerns for toxic or adverse reaction associated with the raw materials for the product and ends with the safe disposal of the end materials from product use. One has only to review the problems associated with the production of vinyl chloride monomer to be concerned about manufacture of products with polyvinyl chloride ( l, 2). If the monomer had been removed from production, many polyvinyl chloride prod­ ucts would no longer be in the market place. In addition, traces of contami­ nants from the manufacture of raw materials often contribute to the potential toxicity of a product (3). Naturally, most concern in safety assessment is directed toward the manufacture and use of products. However, shipping and storage can also contribute to toxic effects at time of use. Also, disposal of end materials of product use causes environmental concerns. Polyvinyl chlo­ ride is also a good example in this case because the release of hydrochloric acid during pyrolysis causes adverse reactions ranging from eye irritation to pulmonary problems including asthma (4, 5).