tbuckley/cabot-search huggingface dataset:
Up-regulation of Tumor Susceptibility Gene 101 Conveys Poor Prognosis through Suppression of p21 Expression in Ovarian Cancer
Authors: Travis W. Young, Daniel G. Rosen, Fang C. Mei, Nan Li, Jinsong Liu, Xiao-Fan Wang, Xiaodong Cheng
Journal: Clinical Cancer Research (2007)

The function of tumor susceptibility gene 101 (TSG101) in ovarian carcinogenesis is largely unexplored. The aim of this study is to investigate the role of TSG101 in human ovarian cancer development, to examine the expression levels of TSG101 in ovarian carcinomas, and to correlate the results with clinicopathologic variables and survival.Human ovarian cancer tissue arrays that contain duplicates of 422 cases of primary ovarian carcinoma were used to probe the expression levels of TSG101 and p21 in epithelial ovarian cancer. In vitro studies in ovarian cancer cells using TSG101-specific small interfering RNA (siRNA) were done to further elucidate the mechanism of TSG101-mediated p21 regulation.We show that TSG101 is increasingly overexpressed in borderline tumors and low-grade and high-grade carcinomas. Patients with low expression of TSG101 survive longer than those with high expression. Suppressing TSG101 by siRNA in ovarian cancer cells led to growth inhibition, cell cycle arrest, and apoptosis with concurrent increases in p21 mRNA and protein. Consistent with this negative association between TSG101 and p21, expression levels of these two markers are inversely correlated in ovarian cancer.TSG101 negatively regulates p21 levels, and up-regulation of TSG101 is associated with poor prognosis in ovarian cancer.
Human autoantibodies that react with both cell nuclei and plasma membranes display specificity for the octamer of histones H2A, H2B, H3, and H4 in high salt.
Authors: O P Rekvig, K Hannestad
Journal: The Journal of Experimental Medicine (1980)

Sera of some patients with systemic lupus erythematosus and related diseases contain a polyclonal antibody population (cross-reactive antinuclear antibodies [X-ANA]) that react specifically with both core mononucleosomes and plasma membranes of viable nucleated cells. Native mononucleosomes and nucleosome cores assembled from long DNA and the inner histones were indistinguishable in terms of inhibition of binding of X-ANA to nuclei of tissue sections and to polynucleosomes on the walls of plastic tubes. In contrast, mononucleosomes selectively depleted of histones H2A and H2B did not inhibit these reactions. A method was developed for isolation of X-ANA from serum that took advantage of the dual specificity of these antibodies. Immunosedimentation in sucrose density gradients revealed that 125I-labeled Fab' fragments of highly pure X-ANA formed complexes with the inner histones H2A, H2B, H3, and H4 in 2 M NaCL, but not in 0.15 M salt. These results indicate that X-ANA recognize an epitope of the inner histone in 2 M salt, and that in 0.15 M NaCL this epitope is not formed unless the histones interact with DNA to generate a nucleosome structure. Furthermore, in light of the previous demonstration that the epitope is destroyed by trypsin, it may be localized in the N-terminal region of histone H2A or H2B.
Home ultraviolet B phototherapy for psoriasis: discrepancy between literature, guidelines, general opinions and actual use. Results of a literature review, a web search, and a questionnaire among dermatologists
Authors: M.B.G. Koek, E. Buskens, C.A.F.M. Bruijnzeel-Koomen, V. Sigurdsson
Journal: British Journal of Dermatology (2006)

Background Home ultraviolet B (UVB) phototherapy is a debated treatment. It is currently being prescribed for patients with psoriasis, although literature on the subject is scarce. Despite the apparent contradiction between clinical practice and literature, no systematic study of either has been conducted.
Decrease of anti-cyclic citrullinated peptide antibodies and rheumatoid factor following anti-TNF therapy (infliximab) in rheumatoid arthritis is associated with clinical improvement
Authors: C Alessandri
Journal: Annals of the Rheumatic Diseases (2004)

To investigate the effect of infliximab treatment on anti-cyclic citrullinated peptide antibodies (anti-CCP) and rheumatoid factor (RF) in patients with rheumatoid arthritis.43 patients with rheumatoid arthritis not responding to disease modifying anti-rheumatic drugs (DMARD) received intravenous infliximab at a dose of 3 mg/kg at baseline and after two and six weeks, and subsequently bimonthly, in combination with methotrexate. Serum samples were collected at baseline and at week 24. A commercial enzyme linked immunosorbent assay was used to test for anti-CCP antibodies; RF were detected using a quantitative nephelometric assay.At baseline, 38 of the 43 patients (88%) were positive for anti-CCP antibodies, and 41 (95%) were positive for RF. The serum titre of anti-CCP and RF decreased significantly after six months of treatment (p = 0.0001 and p<0.0001, respectively). When the patients were grouped on the basis of their clinical response to infliximab, a significant decrease in serum anti-CCP antibodies and RF was observed only in patients who had clinical improvement (ACR 20 and ACR 50).Anti-TNFalpha treatment in rheumatoid arthritis results in a decrease in the serum titres of RF and anti-CCP antibodies in patients showing clinical improvement, suggesting that these measurements may be a useful adjunct in assessing treatment efficacy.
Functional properties of T cell clones with a double specificity for alloantigens and foreign antigens.
Authors: S J Waters, P R Luzzatti, C A Bona
Journal: The Journal of Experimental Medicine (1984)

Four keyhole limpet hemocyanin (KLH)-specific clones prepared from the lymph node of CB6F1 mice immunized with KLH had a proliferative response restricted to parental major histocompatibility complex (MHC)-encoded antigens. These clones provided help for CB6F1 trinitrophenyl-ovalbumin (TNP-OVA)-primed B cells to mount IgM and IgG plaque-forming cell (PFC) responses in the presence of KLH-TNP conjugate. In addition, two of these clones (A12.11 and F6) proliferated in response to allogeneic cells from mice strains bearing H-2k or H-2q haplotypes, respectively. However, they did not provide help for C3H/He or B10.Q primed B cells. The clonal nature of A12.11 and F6 was demonstrated by subcloning and in BUdR-suicide experiments. The proliferative response to KLH was ablated by anti-Iad antibodies, whereas the proliferation induced by C3H/HeJ stimulating cells was ablated by anti-Iak antibodies. Furthermore, both responses were inhibited by a monoclonal anti-clonotype (idiotype) antibody. Taken together, these results strongly support the hypothesis that the same receptor recognizes alloantigens and KLH associated with self-antigens.
Complementary and alternative medicine for functional gastrointestinal disorders
Authors: K Tillisch
Journal: Gut (2006)

Patients suffering from functional gastrointestinal disorders are likely to search elsewhere when conventional therapies fail them. Enthusiasm for complementary and alternative medicine use and research is clearly growing. Studies of acupuncture and herbal therapy for functional gastrointestinal disorders in the Western literature have often been limited by poor study design but these interventions may have promise and are discussed here.
Identifying constituents to participate in a project to control pesticide exposure in children of farmworkers.
Authors: B Thompson, G Coronado, K Puschel, E Allen
Journal: Environmental Health Perspectives (2001)

Farmers in Washington State use pesticides to control harmful pests that might interfere with the quality of their products. Farmworkers, who are primarily responsible for thinning, harvesting, and other agricultural work, are often exposed to these pesticides and take home pesticide residues on their clothing, shoes, and skin, potentially exposing children in the household to pesticides. We designed a project to reduce children's exposure by using a community organization model. To better understand the community views regarding pesticide exposure, we conducted a qualitative community analysis. Two methods of data gathering were used to collect information. Individual interviews were conducted with some respondents, and small group discussions were held with others. Analysis indicated wide disparity among involved groups in their views on pesticides; however, a number of themes common to the majority of the constituents were identified and discussed with a community planning group. Because of the contention around pesticides, the group recommended every constituent participate in decision making. The group noted it was important to emphasize that the research project could provide scientific information on pesticide exposure to everyone in the Lower Yakima Valley. Our study demonstrates both the widely varying views of different constituents around the issue of pesticide exposure and common themes that can form the basis for collaboration and consensus on approaching the issue.
Mechanisms of injuries in World Cup Snowboard Cross: a systematic video analysis of 19 cases
Authors: Arnhild Bakken, Tone Bere, Roald Bahr, Eirik Kristianslund, Lars Nordsletten
Journal: British Journal of Sports Medicine (2011)

Background Snowboard cross (SBX) became an official Olympic sport in 2006. This discipline includes manoeuvring several obstacles while competing in heats. It is common for the riders to collide, making this sport both exciting and at risk of injuries. Although a recent study from the 2010 Olympic Games has shown that the injury risk was high, little is known about the injury mechanisms. Objective To qualitatively describe the injury situation and mechanism of injuries in World Cup Snowboard Cross. Study design Descriptive video analysis. Methods Nineteen video recordings of SBX injuries reported through the International Ski Federation Injury Surveillance System for four World Cup seasons (2006 to 2010) were obtained. Five experts in the field of sports medicine, snowboard and biomechanics performed analyses of each case to describe the injury mechanism in detail (riding situation and rider behaviour). Results Injuries occurred at jumping (n=13), bank turning (n=5) or rollers (n=1). The primary cause of the injuries was a technical error at take-off resulting in a too high jump and subsequent flat-landing. The rider was then unable to recover leading to fall at the time of injury. Injuries at bank turn was characterised by a pattern where the rider in a balanced position lost control due to unintentional contact with another rider. Conclusion Jumping appeared to be the most challenging obstacle in SBX, where a technical error at take-off was the primary cause of the injuries. The second most common inciting event was unintentional board contact between riders at bank turning.
Relationship of Urologic Complications With Health-Related Quality of Life and Perceived Value of Health in Men and Women With Type 1 Diabetes: The Diabetes Control and Complications Trial/Epidemiology of Interventions and Complications (DCCT/EDIC) Cohort
Authors: Alan M. Jacobson, Barbara H. Braffett, Patricia A. Cleary, Rodney L. Dunn, Mary E. Larkin, Hunter Wessells, Aruna V. Sarma
Journal: Diabetes Care (2015)

OBJECTIVE Limited information exists about the influence of urologic complications on health-related quality of life (HRQOL) in patients with type 1 diabetes. RESEARCH DESIGN AND METHODS We studied 664 men and 580 women from the Diabetes Control and Complications Trial/Epidemiology of Interventions and Complications Study: mean ages were 51.6 ± 6.6 and 50.6 ± 7.2 years and duration of diabetes was 29.5 ± 4.8 and 29.8 ± 5.1 years, respectively. We assessed associations of sexual dysfunction, lower urinary tract symptoms (LUTS), and, in women, urinary incontinence (UI) with general quality of life (SF-36), perceived value of health (EuroQol-5), diabetes-related quality of life (Diabetes Quality of Life Scale [DQOL]), and psychiatric symptoms (Symptom Checklist 90-R). RESULTS In both men and women, urologic complications adversely affected HRQOL and psychiatric symptoms, even after accounting for history of depression leading to treatment. Multivariable analyses accounting for the presence of diabetic retinopathy, neuropathy, and nephropathy also revealed substantial independent effects. In men, for example, the odds (95% CI) of a low DQOL score (≤25th percentile) were 3.01 (1.90–4.75) times greater with erectile dysfunction and 2.65 (1.68–4.18) times greater with LUTS and in women, 2.04 (1.25–3.35) times greater with sexual dysfunction and 2.71 (1.72–4.27) times greater with UI/LUTS combined compared with men and women without such complications. Similar effects were observed for the other measures. CONCLUSIONS Sexual dysfunction and urinary complications with type 1 diabetes are associated with decreased quality of life and perceived value of health and with higher levels of psychiatric symptoms, even after accounting for other diabetes complications and depression treatment.
Health-related quality of life in patients with glioblastoma: a randomised controlled trial
Authors: Martin JB Taphoorn, Roger Stupp, Corneel Coens, David Osoba, Rolf Kortmann, Martin J van den Bent, Warren Mason, René O Mirimanoff, Brigitta G Baumert, Elizabeth Eisenhauer, Peter Forsyth, Andrew Bottomley
Journal: The Lancet Oncology (2005)
An Assessment of the Utilization of Complementary and Alternative Medication in Women With Gynecologic or Breast Malignancies
Authors: Marisa A. Navo, Julie Phan, Christy Vaughan, J. Lynn Palmer, Laura Michaud, Kellie L. Jones, Diane C. Bodurka, Karen Basen-Engquist, Gabriel N. Hortobagyi, John J. Kavanagh, Judith A. Smith
Journal: Journal of Clinical Oncology (2004)

Purpose To describe and assess the current utilization of complementary and alternative medicines (CAMs) in women with a diagnosis of either gynecologic or breast cancer and evaluate their reasons for use. Patients and Methods This study included 250 female patients from the Multidisciplinary Breast Center and 250 patients from the Gynecologic Oncology Center of The University of Texas M.D. Anderson Cancer Center (Houston, TX). Patients were selected by having an odd-numbered medical record number, and they were contacted before their clinic visit. The goals of the study were explained, and verbal consent was obtained. Patients who agreed to participate were asked to bring a written list and the medication bottles of all over-the-counter prescriptions and CAMs with them to clinic. In clinic, the investigator obtained a written informed consent and administered the survey. All patients and surveys were assessable. Results The most frequently used herbal products and megavitamins/minerals were identified from the patient medication histories. Overall, we found the proportion of patients using CAM to be 48% (95% CI, 44% to 53%; 241 of 500 patients). CAM use was related to patients' educational status: 62% had postgraduate degrees, 50% had college degrees, 56% had some college, and 33% had a high school education or less. Also, among patients using CAMs, only 53.5% had spoken to a healthcare provider regarding CAM therapy. Conclusion The use of CAM is common among women with cancer. Studies need to be conducted to establish if there are any potential drug interactions and/or therapeutic benefit of CAM products. Moreover, there is a need to educate patients and healthcare providers on appropriate and safe use of CAM products.
Protumorigenic Role of HAPLN1 and Its IgV Domain in Malignant Pleural Mesothelioma
Authors: Alla V. Ivanova, Chandra M.V. Goparaju, Sergey V. Ivanov, Daisuke Nonaka, Christina Cruz, Amanda Beck, Fulvio Lonardo, Anil Wali, Harvey I. Pass
Journal: Clinical Cancer Research (2009)

Abstract Purpose: Tumor extracellular matrix (ECM) plays a crucial role in cancer progression mediating and transforming host-tumor interactions. Targeting the ECM is becoming an increasingly promising therapeutic approach in cancer treatment. We find that one of the ECM proteins, HAPLN1, is overexpressed in the majority of mesotheliomas. This study was designed to characterize the protumorigenic role of HAPLN1 in mesothelioma. Experimental Design: Overexpression of HAPLN1 was assessed and validated on a large set of normal/mesothelioma specimens on the RNA and protein levels. We also analyzed DNA copy number alterations in the HAPLN1 genomic locus using the array-based comparative genomic hybridization representational oligonucleotide microarray analysis tool. Tumorigenic activities of the HAPLN1 domains were evaluated in vitro on mesothelioma cells transfected with HAPLN1-expressing constructs. Results: We found that HAPLN1 is 23-fold overexpressed in stage I mesothelioma and confirmed it for 76% samples (n = 53) on RNA and 97% (n = 40) on protein levels. The majority of lung cancers showed no differential expression of HAPLN1. Analysis of DNA copy number alterations identified recurrent gain in the 5q14.3 HAPLN1 locus in ∼27% of tumors. Noteworthy, high expression of HAPLN1 negatively correlated with time to progression (P = 0.05, log-rank test) and overall survival (P = 0.006). Proliferation, motility, invasion, and soft-agar colony formation assays on mesothelioma cells overexpressing full-length HAPLN1 or its functional domains strongly supported the protumorigenic role of HAPLN1 and its SP-IgV domain. Conclusion: Overexpression of HAPLN1 and its SP-IgV domain increases tumorigenic properties of mesothelioma. Thus, targeting the SP-IgV domain may be one of the therapeutic approaches in cancer treatment.
Regenerative Potential of Embryonic Renal Multipotent Progenitors in Acute Renal Failure
Authors: Elena Lazzeri, Clara Crescioli, Elisa Ronconi, Benedetta Mazzinghi, Costanza Sagrinati, Giuseppe Stefano Netti, Maria Lucia Angelotti, Eliana Parente, Lara Ballerini, Lorenzo Cosmi, Laura Maggi, Loreto Gesualdo, Mario Rotondi, Francesco Annunziato, Enrico Maggi, Laura Lasagni, Mario Serio, Sergio Romagnani, Gabriella Barbara Vannelli, Paola Romagnani
Journal: Journal of the American Society of Nephrology (2007)

Bone marrow-and adult kidney-derived stem/progenitor cells hold promise in the development of therapies for renal failure. Here is reported the identification and characterization of renal multipotent progenitors in human embryonic kidneys that share CD24 and CD133 surface expression with adult renal progenitors and have the capacity for self-renewal and multilineage differentiation. It was found that these CD24+CD133+ cells constitute the early primordial nephron but progressively disappear during nephron development until they become selectively localized to the urinary pole of Bowman's capsule. When isolated and injected into SCID mice with acute renal failure from glycerol-induced rhabdomyolysis, these cells regenerated different portions of the nephron, reduced tissue necrosis and fibrosis, and significantly improved renal function. No tumorigenic potential was observed. It is concluded that CD24+CD133+ cells represent a subset of multipotent embryonic progenitors that persist in human kidneys from early stages of nephrogenesis. The ability of these cells to repair renal damage, together with their apparent lack of tumorigenicity, suggests their potential in the treatment of renal failure.
Short-term outcome after stenting versus endarterectomy for symptomatic carotid stenosis: a preplanned meta-analysis of individual patient data
Authors: Leo H Bonati, Joanna Dobson, Ale Algra, Alain Branchereau, Gilles Chatellier, Gustav Fraedrich, Willem P Mali, Hermann Zeumer, Martin M Brown, Jean-Louis Mas, Peter A Ringleb
Journal: The Lancet (2010)
The Identification of Firearms and Projectiles
Authors: S. Smith
Journal: BMJ (1926)

Aiin.sisted upon by the Government in an attempt to limit the incidence of puerperal sepsis.It is obvious from the writings and speeches of public 1healtlhofficials, as also from those of the laity, either personially or combined in the form of the approved societies, that a great movement is taking place to insist that the present deplorable maternal and infantile mortality and morbidity ought to be, and shall be, diminished.If, therefore, the medical profession itself does not take up the question seriously and now, it will wake up one morning to finid that all kinds of rules and regulations have been made that may seriously affect its position.Let us all do our best; it is no use cursing each other; this will not improve matters; this will not save a single life.Tlhe general practitioner, apart from the patient, is the most seriously concerned.By insisting on ante-natal supervision, by linlking up with the county health authorities, by wel- coming the assistance of obstetric experts, bacteriologists, trained nurses, and voluntary societies, by repprting at once cases of fever-during the puerperinum, by givinlg willinigly all the information he can to those constituted to receive it, the general practitioner is bound to be the chief instrument in the reduction of tho maternal an(d infantile mortality and morbidity.
Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination
Authors: Yanhong Ji, Alicia J. Little, Joydeep K. Banerjee, Bingtao Hao, Eugene M. Oltz, Michael S. Krangel, David G. Schatz
Journal: The Journal of Experimental Medicine (2010)

V(D)J recombination assembles antigen receptor genes in a well-defined order during lymphocyte development. This sequential process has long been understood in the context of the accessibility model, which states that V(D)J recombination is regulated by controlling the ability of the recombination machinery to gain access to its chromosomal substrates. Indeed, many features of “open” chromatin correlate with V(D)J recombination, and promoters and enhancers have been strongly implicated in creating a recombinase-accessible configuration in neighboring chromatin. An important prediction of the accessibility model is that cis-elements and transcription control binding of the recombination-activating gene 1 (RAG1) and RAG2 proteins to their DNA targets. However, this prediction has not been tested directly. In this study, we use mutant Tcra and Tcrb alleles to demonstrate that enhancers control RAG1 binding globally at Jα or Dβ/Jβ gene segments, that promoters and transcription direct RAG1 binding locally, and that RAG1 binding can be targeted in the absence of RAG2. These findings reveal important features of the genetic mechanisms that regulate RAG binding and provide a direct confirmation of the accessibility model.
The Electrocardiographic Findings in Small, Atypical Myocardial Infarcts
Authors: A. HENRY CLAGETT
Journal: Circulation (1958)

Others have described mild or atypical myocardial infarction as a clinical condition in which the pain is of short duration and the evidence of tissue damage is slight. It occurs in otherwise healthy individuals and is characterized by complete recovery and the absence of complications. In this study the electrocardiograms of 359 patients with myocardial infarction were divided according to the QRS complexes; one group showing normal QRS complexes and the other showing typical Q waves. The clinical features of the patients in these two groups were compared.