tbuckley/cabot-search huggingface dataset:
P2-07-01: Copy Number Variants in Early-Onset-Breast Cancer.
Authors: S Ludwig, J Ivanovich, TA Graubert, PJ Goodfellow
Journal: Cancer Research (2011)

Abstract Background: Copy number variants (CNVs) are known to contribute to the risk for development of immune diseases, neurological disorders and to a lesser extent cancer. Many CNVs do not directly involve gene sequences. CHL1, a cell adhesion molecule with homology to L1CAM, is down-regulated in a large proportion of breast cancer. A deletion CNV immediately 5’ of the CHL1 transcriptional start site has been described. The CHL1 CNV's role in breast cancer risk has not previously been explored. Material and Methods: 50 research subjects recruited to the Washington University/Siteman Cancer Center Young Women's Breast Cancer Program (YWBCP) were selected for a CNV discovery study. These women were diagnosed breast cancer &amp;lt; age 40, tested negative for BRCA1/2 mutations and have a negative family history for breast cancer. The selection criteria enriched for de novo mutations. Comparative genomic hybridization (CGH) analyses were undertaken using the NimbleGen 2.1 million oligonucleotide array. DNA copy number was compared between proband and each of her parents to identify potential deletions or amplifications in the daughter relative to both parents (de novo changes). PCR was used to confirm a subset of the CGH-predicted differences in copy number. Chi-square test was used to determine statistical significances of CNV occurrences near the CHL1 gene between women with breast cancer and control cases. Results: aCGH data analysis has been completed for 25 YWBC trios (patients and parents). 247 differences in copy number in the breast cancer cases relative to their parents were identified (1-18 putative de novo CNVs per case). 91% of the observed changes were deletions and 9% were amplifications. One proband showed an 11,560 bp deletion 5’ of the CHL1 gene that was not seen in her parents. Conventional PCR confirmed a homozygous deletion of a ∼3 kb region in the patient, in the region of a known CNV with a reported frequency of ∼3%. Testing an additional 368 early-onset breast cancer cases for the CHL1 deletion revealed 25 additional homozygous deletions (6.8%). The deletion was present in 9 of 197 controls (4.5%). qPCR studies revealed the parents of the patient with the CHL1 deletion each carried a single copy of the CNV, making de novo copy number change in the proband very unlikely. Deletion specific assays for the CHL1 CNV are being used to test CNV genotypes in an extended case-control study. Discussion: CHL1 expression has previously been shown to be down-regulated in primary breast tumors. Our preliminary analysis of YWBC cases and controls indicates the CHL1 regulatory region CNV is unlikely to be associated with risk for early-onset breast cancer. Additional candidate de novo copy number changes seen in the genomes of patients with early-onset breast cancer are being evaluated to determine their roles as genetic risk factors. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P2-07-01.
THU0518 Characteristics, Outcomes, and Predictors of Complementary and Alternative Medicine use in Patients with Rheumatoid Arthritis
Authors: C.-B. Choi, Y.-K. Sung, S.-K. Cho, D.-H. Yoo, S.-S. Lee, J. Lee, J. Kim, H.-S. Lee, T.-H. Kim, B. Y. Yoon, W.-H. Yoo, J.-Y. Choe, S.-H. Lee, S.-C. Shim, W.-T. Chung, S.-J. Hong, C. K. Lee, E. Koh, J.-B. Jun, S.-Y. Bang, S.-K. Kim, H.-S. Cha, J. Lee, J. Shim, S.-C. Bae
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Use of complementary and alternative medicine (CAM) is not uncommon in patients with rheumatoid arthritis (RA), but patient characteristics and the factors influencing and predicting its use is not well known. <h3>Objectives</h3> We aimed to assess the characteristics and outcomes of CAM in patients with RA and to analyze the predicting factor for its use. <h3>Methods</h3> A total of 5,360 RA patients from the KORONA (Korean Observational Study Network for Arthritis) prospective multicenter cohort were assessed for use of CAM and characteristics including age, sex, disease duration, delay in diagnosis, family history, comorbidities, socioeconomic status, fatigue, sleep, pain, exercise, smoking, HAQ, EQ-5D, DAS28, medication, radiographic damage, surgery, and adverse events. Multiple logistic regression was used to analyze the predicting factors for CAM use. <h3>Results</h3> Of the 5,360 patients, 2,468 (46.0%) patients responded that they have used CAM. Acupuncture was most common (73.3%) followed by herbal medicine (60.4%), moxibustion (30.7%), bee venom (19.1%), others (7.7%), and placenta injection (3.9%). Female used CAM significantly more and CAM users were significantly younger at onset (p&lt;0.01), had longer disease duration (p&lt;0.01), more family history (p=0.02), lower income (p&lt;0.01), and lower education status (p&lt;0.01). CAM users reported significantly more fatigue (p&lt;0.01), more sleep disturbance (p&lt;0.01), more pain (p&lt;0.01), worse general condition (p&lt;0.01), higher HAQ score (p&lt;0.01), and lower EQ-5D score (p&lt;0.01). They also had significantly higher disease activity (p&lt;0.01, more use of steroids (p&lt;0.01) and NSAIDs (p&lt;0.01), had more radiographic damage (p&lt;0.01), underwent more surgery (p&lt;0.01), and had more adverse events (p&lt;0.01). One or more problem in any of the dimensions of EQ-5D was significantly associated with the use of CAM. Factors significantly predicting the use of CAM were female gender (OR, 95%CI, 1.71, 1.30-2.45), older age, steroids (1.49, 1.29-1.72), adverse events (1.43, 1.26-1.63), radiographic damage (1.40, 1.21-1.62), regular exercise (1.35, 1.19-1.53), surgery (1.31, 1.10-1.55), high income (1.30, 1.06-1.60), and high DAS28 (1.07, 1.07-1.15). <h3>Conclusions</h3> Patients with higher disease activity and lower health-related quality of life and higher functional disability used CAM significantly more frequently. <h3>Acknowledgements</h3> This study is supported by a grant from the Korea Healthcare Technology R&amp;D Project, Ministry of Health and Welfare, Republic of Korea (A102065). <h3>Disclosure of Interest</h3> None Declared
Relation of Respiratory Water Loss to Coughing after Exercise
Authors: Arthur S. Banner, Joseph Green, Margarette O'Connor
Journal: New England Journal of Medicine (1984)

Exercise has been shown to result in bronchoconstriction, the extent of which is related to respiratory heat loss. Some normal subjects report coughing after exercise, and to determine whether this might also be related to respiratory heat loss, we monitored cough frequency after hyperpnea with air of varying temperature and water content in seven such subjects. Hyperpnea with fully saturated air at 37 degrees failed to provoke coughing and was not associated with heat loss or water loss. Hyperpnea with dry air at 37 degrees C resulted in more water loss (P less than 0.0005), less heat loss (P less than 0.01), and more coughing (P less than 0.001) than hyperpnea with subfreezing air. Hyperpnea with ambient air was associated with a similar cough frequency and water loss but with less heat loss (P less than 0.001) than hyperpnea with subfreezing air. There was a direct relation between cough frequency and respiratory water loss but no consistent relation between cough frequency and respiratory heat loss. This study indicates that coughing after hyperpnea with poorly conditioned air is related to the overall rate of respiratory water loss. Since strenuous exercise is associated with marked hyperpnea, coughing after exercise may also result from respiratory water loss.
LACK OF ASSOCIATION BETWEEN EPICARDIAL FAT VOLUME AND EXTENT OF CORONARY ARTERY CALCIFICATION, SEVERITY OF CORONARY ARTERY DISEASE, OR PRESENCE OF MYOCARDIAL PERFUSION ABNORMALITIES IN A DIVERSE, SYMPTOMATIC PATIENT POPULATION: RESULTS FROM THE CORE320 MULTICENTER STUDY
Authors: Armin Arbab-Zadeh, Yutaka Tanami, Masahiro Jinzaki, Satoru Kishi, Mathew Matheson, Andrea Vavere, Carlos Rochitte, Marc Dewey, Marcus Chen, Melvin Clouse, Christopher Cox, Sachio Kuribayashi, Joao Lima
Journal: Journal of the American College of Cardiology (2015)
Ceco-colic intussusception with subsequent bowel infarction as a rare complication of colonoscopic polypectomy
Authors: C. Lee, J. Shim, J. Jang
Journal: Endoscopy (2013)

A 47-year-old man underwent a colonoscopic polypectomy to treat a cecal adenoma detected during a screening colonoscopy. The initial colonoscopy showed a 15-mm, nonpolypoid lesion (0-IIa type) located at the cecal base ([Fig. 1]). After a submucosal injection of 4 mL of diluted epinephrine solution (1 : 10 000), the lesion was successfully resected en bloc using a standard snare and electrical current and without complications. Prophylactic hemoclipping was carried out to prevent postprocedural bleeding. Total time taken for the colonoscopy was about 14 minutes.
Abstract 3473: Endothelin axis autocrine loop is positively regulated by the interplay between ET-1 and hypoxia-inducible factor-1α in melanoma cells
Authors: Francesca Spinella, Valentina Caprara, Roberta Cianfrocca, Laura Rosanò, Valeriana DiCastro, Pier Giorgio Natali, Anna Bagnato
Journal: Cancer Research (2011)

Abstract Phenotypic and genotypic analyses of cutaneous melanoma have identified the endothelin B receptor (ETBR) as tumor progression marker, thus representing a potential therapeutic target. We previously demonstrated that ETBR activation by endothelin (ET)-1, promotes melanoma progression and lymphatic vessel growth through a hypoxia-inducible factor (HIF)-1α-mediated mechanism and cooperates with hypoxia to increase this effect. In this study we show that in melanoma cells ETs increase ET-1 and ETBR mRNA and protein expression and their promoter activity that were completely blocked by the selective ETBR antagonist, BQ788, supporting the presence of a positive ET-1-autocrine loop. Moreover when melanoma cells were exposed to hypoxia the induction of both ETBR and ET-1 was markedly enhanced demonstrating that hypoxia potentiates the ET-1-autocrine loop by reinforcing the expression of ETBR and the secretion of ET-1 that, in turn, increases simultaneously ET-1/ETBR expression. Silencing HIF-1α or HIF-2α significantly reduced the promoter activity and the expression of ET-1 and ETBR in response to hypoxia or ETs. Chromatin immunoprecipitation assay showed increased HIF-1α and HIF-2α binding to ET-1 and ETBR promoter in response to hypoxia or ET-1 stimulation. Interestingly, ETs through ETBR enhance both vascular endothelial growth factor (VEGF)-A and VEGF-C mRNA and protein expression and promoter activity. At functional level silencing of HIF-1α and HIF-2α inhibited the ET-1 and hypoxia-induced VEGF-A and VEGF-C expression, providing that hypoxia and ET-1 axis share the same transcriptional machinery through which may control angiogenic and lymphangiogenic response. Moreover, in melanoma xenografts, ETBR antagonist suppressed tumor growth, neovascularization-related effectors, indicating that targeting ETBR related signalling cascade may represent a novel treatment of melanoma by impairing the positive feedback loop between ET axis and hypoxic melanoma microenvironment. Supported by AIRC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3473. doi:10.1158/1538-7445.AM2011-3473
P15 Directional Next Generation Whole Transcriptome Sequencing Of Primary Human Pulmonary Microvascular Endothelial Cells
Authors: C. Shovlin, D. Patel, F. Govani, A. Giess, M. Jones, L. Game, I. Mollet
Journal: Thorax (2014)

<h3>Introduction and objectives</h3> To improve our understanding of the dynamic interplay between non-coding and coding RNA species, we developed solution-based methods to capture the entire endothelial transcriptome. In contrast to microarrays and CHIP-based methodologies, there was no pre-specification of RNA target sequences, and the identity of the DNA strand of origin was preserved. <h3>Methods</h3> Triplicate confluent primary human microvascular endothelial cells (Promocell GmbH) were cultured in the presence and absence of TGF-beta stimuli relevant to pulmonary arteriovenous malformations and hereditary haemorrhagic telangiectasia (HHT). Following rRNA-depletion of total RNA, seven libraries were prepared using Illumina reagents, and 8 pM of each library used for cluster generation and sequencing on Genome Analyser II. Algorithms for aligning reads to NCBI36/hg18 and GRCh37/hg19 included Bowtie, TopHat and Seqmap. Validations were performed using quantitative rt-PCR. <h3>Results</h3> More than 2 Gigabases of sequence was generated. Transcriptome-wide profiles were similar between libraries, with sixteen types of RNA species detected including 146 micro (mi)RNA families (47 broadly conserved), and 10,749 protein-encoding mRNAs representing ~5.5% of mapped reads. Alignments to endothelial mRNAs/miRNAs were substantially higher than to gene loci for non-endothelial mRNAs/miRNAs. mRNA exon alignments demonstrated sharp exon boundary delineation, but replicate alignments to non-repetitive intronic regions involved in multi-exon deletions in HHT patients. There was an inverse relationship between alignments depths and qt-PCT cycle thresholds (Ct), where single alignments were detectable, and Ct values of 20 generated by 0.02 nM spiked RNA. Across all experiments in replicate donor/treatment RNAs, for a panel of single open reading frame miRNA genes, RNASeq alignments (gene strand read counts normalised to the total number of valid reads and exon/locus size) explained 72% of the variance of qt-PCR cycle threshold (p &lt; 0.0001). Dynamic whole transcriptome profiling is in progress. <h3>Conclusions</h3> These novel directional next generation RNA sequencing methods provide new insights for mutational mechanisms, and a systems approach to dissection of regulatory and target RNA networks relevant to human disease.
Isolation of HTLV-III from Cerebrospinal Fluid and Neural Tissues of Patients with Neurologic Syndromes Related to the Acquired Immunodeficiency Syndrome
Authors: David D. Ho, Teresa R. Rota, Robert T. Schooley, Joan C. Kaplan, J. Davis Allan, Jerome E. Groopman, Lionel Resnick, Donna Felsenstein, Charla A. Andrews, Martin S. Hirsch
Journal: New England Journal of Medicine (1985)

Original Article from The New England Journal of Medicine — Isolation of HTLV-III from Cerebrospinal Fluid and Neural Tissues of Patients with Neurologic Syndromes Related to the Acquired Immunodeficiency Syndrome
Prophylactic Efficacy of Nitrofurantoin Macrocrystals and Trimethoprim-Sulfamethoxazole in Urinary Infections
Authors: Thomas A. Stamey, Mercy Condy, Gladys Mihara
Journal: New England Journal of Medicine (1977)

We examined bacterial persistence and resistance in the vaginal and fecal flora of 28 women who received a total of 253 months of daily low-dosage prophylaxis with nitrofurantoin macrocystals (100 mg per day) or trimethoprim–sulfamethoxazole (40 and 200 mg respectively). During trimethoprim–sulfamethoxazole prophylaxis, 8.5 per cent of monthly vaginal and 27 per cent of monthly fecal cultures grew Escherichia coli, as compared to 36 and 96 per cent, respectively, for nitrofurantoin macrocrystals. Esch. coli resistant to nitrofurantoin occurred in two of 95 fecal cultures during therapy, and Esch. coli resistant to trimethoprim–sulfamethoxazole in 16 of 182 (8.8 per cent) fecal cultures. In the 12 months before treatment, 92 episodes of recurrent urinary infection occurred, as compared to six during treatment (none occurred on trimethoprim–sulfamethoxazole). These results confirm the value of trimethoprim–sulfamethoxazole prophylaxis for high-risk patients with recurrent urinary infections. Prophylaxis with nitrofurantoin macrocrystals seems adequate for less severe problems of reinfection. (N Engl J Med 296:780–783, 1977)
THE EFFECT OF MINDFULNESS-BASED STRESS REDUCTION ON ANGINA AND VASCULAR FUNCTION IN WOMEN WITH NON-OBSTRUCTIVE CORONARY ARTERY DISEASE
Authors: Vedant Pargaonkar, Joshua Goldner, Katharine S. Edwards, Philippe R. Goldin, Bonnie Zimmerman, Jennifer Tremmel
Journal: Journal of the American College of Cardiology (2015)
Efficient and safe esophageal endoscopic submucosal dissection using inverted overtube after changing patient position
Authors: Hirohito Mori, Hideki Kobara, Noriko Nishiyama, Shintaro Fujihara, Tae Matsunaga, Maki Ayaki, Tatsuo Yachida, Takaaki Tsushimi, Tsutomu Masaki
Journal: Endoscopy (2014)

During esophageal endoscopic submucosal dissection (ESD), a left-sided lesion may be affected by the direction of gravity, which causes pooling of water, blood, and tiny resection fragments that obscure the lesion [1]. We used a new inverted endoscopic overtube (Endo Rescue, TOP Co. Tokyo, Japan) to perform esophageal ESD more effectively and safely in left-sided lesions ([Fig. 1]).
TCT-741 Risk Stratification and Clinical Pathways Optimize Length of Stay after Transfemoral Transcatheter Aortic Valve Replacement
Authors: Sandra Lauck, David A. Wood, Anson Cheung, Richard Cook, Danny Dvir, Mathieu Lempereur, Imran A. Shiekh, Dion Stub, John Webb, Peter Fahmy, Jian Ye
Journal: Journal of the American College of Cardiology (2014)
Korea's Thyroid-Cancer “Epidemic” — Screening and Overdiagnosis
Authors: Hyeong Sik Ahn, Hyun Jung Kim, H. Gilbert Welch
Journal: New England Journal of Medicine (2014)

In 2011, the rate of thyroid-cancer diagnoses in the Republic of Korea was 15 times that observed in 1993, yet thyroid-cancer mortality remains stable — a combination that suggests that the problem is overdiagnosis attributable to widespread thyroid-cancer screening.
Abstract 44: STAT1-dependent expression of genes and proteins involved in energy metabolism links tumor growth and radioresistance to the Warburg effect and predicts poor prognosis in cancer patients
Authors: Nikolai N. Khodarev, Sean P. Pitroda, Bassam T. Wakim, Daniel W. Golden, Michael A. Beckett, Helena J. Mauceri, Harold G. Sutton, Samantha Perakis, Renuku Malik, Christian Wietholt, Charles Pelizzari, Chin-Tu Chen, Ralph R. Weichselbaum
Journal: Cancer Research (2010)

Abstract Signal Transducer and Activator of Transcription 1 (STAT1) has traditionally been regarded as a transmitter of interferon signaling and a pro-apoptotic tumor suppressor. Recent data have identified new functions of STAT1 associated with tumorigenesis and resistance to genotoxic stress, including ionizing radiation (IR) and chemotherapy. To investigate the mechanisms contributing to the tumorigenic functions of STAT1, we performed a combined transcriptomic-proteomic expressional analysis of tumor xenografts with stable Stat1 knock-down (KD) relative to tumors with wild type (wt) Stat1. We also compared previously described SCC61 and nu61 isologous tumors, where SCC61 is radiosensitive and nu61 is radioresistant counterparts with different expression of IFN/Stat1 pathway. Transcriptional profiling was based on Affymetrix Human GeneChip® Gene 1.0 ST microarrays. Proteomes were determined from the MS/MS data by searching against the human subset of the UniProt database. Knockdown of STAT1 led to significant growth suppression in untreated tumors and radiosensitization of irradiated tumors. These changes were accompanied by alterations in the expression of genes and proteins of glycolysis/gluconeogenesis (GG), the citrate cycle (CC), and oxidative phosphorylation (OP). Of these pathways, GG had the most concordant changes in gene and protein expression and demonstrated a STAT1-dependent expression of genes and proteins consistent with tumor-specific glycolysis. IR drastically suppressed the GG pathway in STAT1 KD tumors without significant change in STAT1 WT tumors. Using 18F-FDG-PET we observed significantly higher glucose uptake in nu61 compared to SCC61 post-irradiation (6Gy). The STAT1 and glycolytic pathways were co-expressed in human breast tumors, and expression of STAT1-linked glycolytic genes was highly predictive of poor prognosis. Our results identify a previously uncharacterized function of STAT1 in tumors: expressional regulation of genes and enzymes involved in glycolysis, the citrate cycle, and mitochondrial oxidative phosphorylation, with predominant regulation of glycolysis. STAT1-dependent transcriptional and translational regulation of glycolysis suggests a potential role for STAT1 as a transcriptional modulator of genes responsible for the Warburg effect. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 44.
Tc-99m Sestamibi and Tc-99m HMDP Uptake in a Malignant Phyllodes Tumor of the Breast
Authors: H Ohta, S Yamamoto, M Ukikusa, H Awane, M Shintaku, K Irie
Journal: Clinical Nuclear Medicine (1997)

Log in or Register Subscribe to journalSubscribe Get new issue alertsGet alerts Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed to maintaining your privacy and will not share your personal information without your express consent. For more information, please refer to our Privacy Policy. Subscribe to eTOC Secondary Logo Journal Logo All Articles Images Videos Podcasts Blogs Advanced Search Toggle navigation Subscribe Register Login Articles & Issues Current IssuePrevious IssuesPublished Online First Collections ACNM MeetingsCMECommunication in OrganizationCOVID-19Feedback and RewardsFrom the American College of Nuclear MedicineHighly Cited and Frequently Read ArticlesPlanning and Conducting Meetings EffectivelyQuality and Safety in Health CareRecruitment of New PhysiciansTips for Giving a Memorable PresentationTips for LeadersTips for Physicians in Getting the Right Job ACNM ArticlesACNM Website For Authors Submit a ManuscriptInformation for AuthorsLanguage Editing ServicesAuthor Permissions Journal Info About the JournalEditorial BoardAdvertisingOpen AccessSubscription ServicesReprintsRights and Permissions All Articles Images Videos Podcasts Blogs Advanced Search
Endoscopic guide wire gallbladder decompression in a critically ill, septic patient
Authors: G. Curcio, N. Azzopardi, A. Granata, L. Barresi, I. Tarantino, M. Traina
Journal: Endoscopy (2013)

A 57-year-old man with postischemic dilated cardiomyopathy underwent a heart transplant at our center in February 2013. His early postoperative course was complicated by primary graft non-function requiring intensive care treatment. Six days after transplantation, he developed severe hypoperfusion that required maximal extracorporeal membrane oxygenation (ECMO), placement of an intra-aortic balloon pump, and vasoactive drug support.
Urinary Pyridinium Cross-Links: A Noninvasive Diagnostic Test for Ehlers-Danlos Syndrome Type VI
Authors: Marzia Pasquali, Philip P. Dembure, Maria J. Still, Louis J. Elsas
Journal: New England Journal of Medicine (1994)

To the Editor: Ehlers-Danlos syndrome type VI is an autosomal recessive disorder of connective tissue caused by mutations in the gene for collagen lysyl hydroxylase. This enzyme hydroxylates lysyl residues on newly synthesized intracellular collagen peptides, and its impairment results in a low hydroxylysine content in mature collagen1,2. Diagnosis has required a large skin-biopsy specimen to demonstrate reduced amounts of hydroxylysine in skin collagen and defective lysyl hydroxylase in cultured skin fibroblasts. Here, we report that measurement of the urinary excretion of pyridinium cross-links serves as a simple, noninvasive diagnostic test for this disorder. After extracellular secretion, specific . . .