tbuckley/cabot-search huggingface dataset:
LSC 2012 abstract - Age-related changes in the relative expression of functional genes in mesenchymal stem cells
Authors: Martha Bustos, Maria Kapetanaki, Ernest Meyer, Albert Donnenberg, Lyle Mroz, Naftali Kaminski, Ana Mora, Mauricio Rojas
Journal: European Respiratory Journal (2012)

Age-associated changes increased susceptibility to a variety of lung pathologies. Recently, mesenchymal stem cells or B-MSCs have emerged as a critical reparative response mechanism to lung injury. We had demonstrated an association, in animal models, between age and an increase in the susceptibility to injury and we had identified functional differences between B-MSCs from young and old mice.In the present study, we examined the consequences of aging in the gene expression.

METHODS: 3 and 24 months old B6 mice were sacrificed and B-MSC were isolated according to the expression of the surface markers. RNA was obtained after sorting and used for hybridization on Agilent gene expression microarrays. Ingenuity software was used to determine functional enrichment. For statistical analysis we applied significance analysis of microarrays. A qvalue of 5, which corresponds to a 5% false discovery rate, was used as a cutoff of statistical significance. Gene validations were performed by RT-PCR.

RESULTS: We demonstrate that aging induce a decrease in the gene expression on B-MSC. The mechanisms affected by the decrease on gene expression includecellular trafficking, cellular growth and proliferation.

CONCLUSION: Old B-MSCs have a different expression profile that exhibits a decrease in the expression of genes that control importan B-MSC functions.
Regulation of calcium current by intracellular calcium in smooth muscle cells of rabbit portal vein.
Authors: Y Ohya, K Kitamura, H Kuriyama
Journal: Circulation Research (1988)

Effects of concentrations of intracellular calcium, [Ca2+]i, on the voltage-dependent Ca2+ current (ICa) recorded from dispersed single smooth muscle cells of the rabbit portal vein were studied, using a whole cell voltage clamp method combined with an intracellular perfusion technique. Outward currents were minimized by replacement of Cs+ -rich solution in the pipette and 20 mM tetraethylammonium in the bath. The ICa was evoked by command pulses of above -30 mV, and the maximum amplitude was obtained at about 0 mV. This ICa was dose dependently inhibited by increases in the [Ca2+]i above 30 nM. The Kd value of the [Ca2+]i required to inhibit the ICa was about 100 nM. The Ba2+ current was also inhibited by increases in the [Ca2+]i. Conversely, perfusion of Ba2+ into the cell up to 100 microM did not suppress the ICa. Changes in the [Ca2+]i did not modify the steady-state inactivation curve. The inhibition of the ICa evoked by the test pulse is most prominent when the preceding influx of Ca2+ during the conditioning pulse was large, as estimated using a double pulse protocol. This inhibition was proportionally reduced by increases in the concentration of the Ca2+ chelator, ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA). Therefore, the Ca2+ -dependent inactivation of the Ca2+ channel may contribute toward regulating [Ca2+]i in smooth muscle cells of the rabbit portal vein.
Treatment for painful calcified chronic pancreatitis: extracorporeal shock wave lithotripsy versus endoscopic treatment: a randomised controlled trial
Authors: J.-M. Dumonceau, G. Costamagna, A. Tringali, K. Vahedi, M. Delhaye, A. Hittelet, G. Spera, E. Giostra, M. Mutignani, V. De Maertelaer, J. Deviere
Journal: Gut (2006)

In chronic pancreatitis, obstruction of the main pancreatic duct (MPD) may contribute to the pathogenesis of pain. Pilot studies suggest that extracorporeal shock wave lithotripsy (ESWL) alone relieves pain in calcified chronic pancreatitis.To compare ESWL alone with ESWL and endoscopic drainage of the MPD for treatment of pain in chronic pancreatitis.Patients with uncomplicated painful chronic pancreatitis and calcifications obstructing the MPD.55 patients were randomised to ESWL alone (n = 26) or ESWL combined with endoscopy (n = 29).2 years after trial intervention, 10 (38%) and 13 (45%) patients of the ESWL alone and ESWL combined with endoscopy group, respectively, had presented pain relapse (primary outcome) (OR 0.77; 95% CI 0.23 to 2.57). In both groups, a similar decrease was seen after treatment in the MPD diameter (mean decrease 1.7 mm; 95% CI 0.9 to 2.6; p<0.001), and in the number of pain episodes/year (mean decrease, 3.7; 95% CI 2.6 to 4.9; p<0.001). Treatment costs per patient were three times higher in the ESWL combined with endoscopy group compared with the ESWL alone group (p = 0.001). The median delay between the onset of chronic pancreatitis and persistent pain relief for both groups was 1.1 year (95% CI 0.7 to 1.6), as compared with 4 years (95% CI 3 to 4) for the natural history of chronic pancreatitis in a reference cohort (p<0.001).ESWL is a safe and effective preferred treatment for selected patients with painful calcified chronic pancreatitis. Combining systematic endoscopy with ESWL adds to the cost of patient care, without improving the outcome of pancreatic pain.
Evidence for Increased Serum Glutamic Oxalacetic Transaminase (SGO-T) Activity Following Graded Myocardial Infarcts in Dogs
Authors: IRWIN NYDICK, FELIX WRóBLEWSKI, JOHN S. LADUE
Journal: Circulation (1955)

Elevation in serum concentration of the enzyme, glutamic oxalacetic transaminase, (SGO-T), is an accurate index of myocardial infarction in dogs. The enzyme rises in a rough proportion to the extent of myocardial necrosis. The test is a sensitive guide to myocardial injury. Infarcts less than 1 Gm. in weight result in a significant alteration in serum concentration of the enzyme. Concentrations of the enzyme in infarcted muscle are markedly lower than in normal muscle, suggesting that seepage through damaged cells causes the rise in serum concentration. Experimental and clinical myocardial injury result in similar abnormalities.
Repeated sauna treatment improves vascular endothelial and cardiac function in patients with chronic heart failure
Authors: Takashi Kihara, Sadatoshi Biro, Masakazu Imamura, Shiro Yoshifuku, Kunitsugu Takasaki, Yoshiyuki Ikeda, Yutaka Otuji, Shinichi Minagoe, Yoshifumi Toyama, Chuwa Tei
Journal: Journal of the American College of Cardiology (2002)
The Annual Oration, on Abdominal Section for the Treatment of Intra-Peritoneal Injury
Authors: W. Mac Cormac
Journal: BMJ (1887)

occurrence of certain diseases.Thhe attendance this afternoon shows a general interest in this movement, and argues well for the formation and prosperity of the society, which I trust will prove long-lived and successful.I will occupy your time no looger, for the relation between brevity of life and the length of time; and the value of brevity, in words as well as in action, are becoming daily more manifest in the quickly shifting scenes of this singularly mobile age.
1160-170 Irbesartan significantly reduces C-reactive protein after one month of therapy in unstable angina
Authors: Mariaelena Lombardi, Luigi M Biasucci, Maddalena Piro, Giovanna Di Giannuario, Giulia Aguzzi, Sonia Iacovella, Giovanna Liuzzo, Filippo Crea
Journal: Journal of the American College of Cardiology (2004)
The concept of “normal” movement and its consequences for therapy
Authors: Jürgen Konczak, Johannes Dichgans
Journal: Behavioral and Brain Sciences (1996)

Abstract The guideline for therapy should be the improvement of the individual's functionality, not the acquisition of the ill-defined “goldstandard” of normal movement. However, Latash &amp; Anson's suggestion that only primary causes of dysfunction should be treated is problematic for two reasons: First, the distinction between genuine and adaptive changes in motor performance is not always possible, and second, adaptive changes do not necessarily improve motor function, but may actually be detrimental to the system's performance.
Sympathetic Reinnervation of the Sinus Node and Exercise Hemodynamics After Cardiac Transplantation
Authors: Robert F. Wilson, Thomas H. Johnson, George C. Haidet, Spencer H. Kubo, Marcus Mianuelli
Journal: Circulation (2000)

Sympathetic cardiac reinnervation occurs variably after cardiac transplantation (CT) in humans. We hypothesized that sinus node reinnervation would partially restore normal chronotropic response to exercise.Thirteen recent CT recipients, 28 late CT recipients (> or =1 year after CT), and 20 control subjects were studied. Sinus node sympathetic reinnervation was determined by heart rate (HR) change after tyramine injection into the artery that perfused the sinus node. HR changes of <5 and > or =15 bpm were defined, respectively, as denervation and marked reinnervation. During treadmill exercise, HR, blood pressure, and expired O(2) and CO(2) were measured. All early transplant recipients exhibited features typical of denervation (basal HR, 88+/-2 bpm; peak HR, 132+/-4 bpm, peaking 1.8+/-0.3 minutes after exercise cessation and slowly declining after exercise). A similar pattern was found in the 12 late transplant recipients with persistent sinus node denervation. However, in patients with marked reinnervation, exercise HR rose more (peak HR, 142+/-4 and 141+/-2 bpm), peaked earlier after cessation of exercise (0.7+/-0.4 and 0. 3+/-0.1 minute), and fell more rapidly. Exercise duration and maximal oxygen consumption were not related significantly to reinnervation status, but a trend existed for longer exercise time in markedly reinnervated patients.The present studies suggest that sympathetic reinnervation of the sinus node is accompanied by partial restoration of normal HR response to exercise. Both maximal oxygen consumption and exercise duration were markedly shorter in CT patients than in control subjects, and most of the difference was not related to innervation status.
Long-Term Results of Anti– Helicobacter pylori Therapy in Early-Stage Gastric High-Grade Transformed MALT Lymphoma
Authors: Li-Tzong Chen, Jaw-Town Lin, John Jen Tai, Gran-Hum Chen, Hong-Zen Yeh, Sheng-Shun Yang, Hsiu-Po Wang, Sung-Hsin Kuo, Bor-Shyang Sheu, Chang-Ming Jan, Wen-Ming Wang, Tsang-En Wang, Chew-Wun Wu, Chi-Long Chen, Ih-Jen Su, Jacqueline Whang-Peng, Ann-Lii Cheng
Journal: JNCI Journal of the National Cancer Institute (2005)

Background: Several independent clinical studies have reported that Helicobacter pylori eradication therapy could achieve complete remission in some patients with H. pylori –positive early-stage gastric mucosa–associated lymphoid tissue (MALT) lymphoma. Methods: To compare the long-term results of anti– H. pylori therapy in early-stage, gastric low-grade and high-grade transformed MALT lymphoma, two multicenter prospective studies of anti– H. pylori therapy for early-stage gastric lymphoma conducted in Taiwan, one for low-grade MALT lymphoma, with 34 patients enrolled from March 1996 through April 1999, and one for high-grade transformed tumors (diffuse large B-cell lymphoma with features of MALT, DLBCL[MALT] lymphoma), with 24 patients enrolled since June 1995, were directly compared. In both studies, patients generally received 2 weeks of antibiotics and had multiple sequential follow-up endoscopic examinations until complete histologic remission (CR) or disease progression; patients were monitored through January 31, 2004. CR was defined as regression of lymphoid infiltration to Wotherspoon's score of 2 or less on all pathologic sections of endoscopic biopsy specimens. All statistical tests were two-sided. Results: The H. pylori –positive rate among the 34 low-grade patients was 94% (32 of 34). All 24 selected high-grade patients were H. pylori positive. H. pylori was eradicated in 97% (30 of 31) of evaluable H. pylori –positive low-grade patients and in 92% (22 of 24) of high-grade patients, which led to CR in 80% (24 of 30, 95% confidence interval [CI] = 65% to 95%) and 64% (14 of 22, 95% CI = 42% to 86%) of patients, respectively. None of the five patients who were either initially H. pylori negative or had persistent H. pylori infection after antibiotics achieved CR. After median follow-up of more than 5 years in complete responders, tumor recurrence was observed in three (13%) low-grade patients but not in high-grade patients. Conclusions: Anti– H. pylori therapy may be considered as one of the treatment options for early-stage H. pylori –positive gastric DLBCL(MALT), and large-scale prospective studies to validate its use as first-line therapy for such tumors should be undertaken.
The Nonhematopoietic Effects of Erythropoietin in Skin Regeneration and Repair: From Basic Research to Clinical Use
Authors: Heiko Sorg, Yves Harder, Christian Krueger, Kerstin Reimers, Peter M. Vogt
Journal: Medicinal Research Reviews (2012)

Abstract Erythropoietin ( EPO ) is the main regulator of red blood cell production but there exists also a variety of nonhematopoietic properties. More recent data show that EPO is also associated with the protection of tissues suffering from ischemia and reperfusion injury as well as with improved regeneration in various organ systems, in particular the skin. This review highlights the mechanisms of EPO in the different stages of wound healing and the reparative processes in the skin emphasizing pathophysiological mechanisms and potential clinical applications. There is clear evidence that EPO effectively influences all wound‐healing phases in a dose‐dependent manner. This includes inflammation, tissue, and blood vessel formation as well as the remodeling of the wound. The molecular mechanism is predominantly based on an increased expression of the endothelial and inducible nitric oxide ( NO ) synthase with a consecutive rapid supply of NO as well as an increased content of vascular endothelial growth factor ( VEGF ) in the wound. The improved understanding of the functions and regulatory mechanisms of EPO in the context of wound‐healing problems and ischemia/reperfusion injury, especially during flap surgery, may lead to new considerations of this growth hormone for its regular clinical application in patients.
Guidelines in cardiac clinical practice: evaluation of their methodological quality using the AGREE II instrument
Authors: Sanjeeve Sabharwal, Vanash Patel, Sukhjinder S Nijjer, Ali Kirresh, Ara Darzi, John C Chambers, Iqbal Malik, Jaspal S Kooner, Thanos Athanasiou
Journal: Journal of the Royal Society of Medicine (2013)

Although clinical guidelines have an influential role in healthcare practice, their development process and the evidence they cite has been subject to criticism. This study evaluates the quality of guidelines in cardiac clinical practice by examining how they adhere to validated methodological standards in guideline development. A structured review of cardiac clinical practice guidelines published in seven cardiovascular journals between January 2001 and May 2011 was performed. The AGREE II assessment tool was used by two researchers to evaluate guideline quality. A total of 101 guidelines were identified. Assessment of guidelines using AGREE II found methodological quality to be highly variable (median score, 58.70%; range, 45.34–76.40%). ‘Scope and purpose’ (median score, 86.1%) and ‘clarity of development’ (median score, 83.3 %) were the two domains within AGREE II that received the highest scores. Applicability (median score, 20.80%; range, 4.20–54.20%) and editorial independence (median score, 33.30%; range, 0–62.50%) had the lowest scores. There is considerable variability in the quality of cardiac clinical practice guidelines and this has not improved over the last 10 years. Incorporating validated guideline assessment tools, such as AGREE II, may improve the quality of guidelines.
Baicalein, a component of Scutellaria radix from Huang-Lian-Jie-Du-Tang (HLJDT), leads to suppression of proliferation and induction of apoptosis in human myeloma cells
Authors: Zi Ma, Ken-ichiro Otsuyama, Shangqin Liu, Saeid Abroun, Hideaki Ishikawa, Naohiro Tsuyama, Masanori Obata, Fu-Jun Li, Xu Zheng, Yasuko Maki, Koji Miyamoto, Michio M. Kawano
Journal: Blood (2004)
Resolution of Infectious Parameters after Antimicrobial Therapy in Patients with Ventilator-associated Pneumonia
Authors: PAUL J. W. DENNESEN, ANDRÉ J. A. M. van der VEN, ALPHONS G. H. KESSELS, GRAHAM RAMSAY, MARC J. M. BONTEN
Journal: American Journal of Respiratory and Critical Care Medicine (2001)

Although recommended durations of antimicrobial therapy for ventilator-associated pneumonia (VAP) range from 7 to 21 d, these are not based on prospective studies and little is known about the resolution of symptoms after start of antibiotics. Resolution of these symptoms was investigated in 27 patients. VAP was diagnosed on clinical, radiographic, and microbiological criteria, including quantitative cultures of bronchoalveolar lavage. All patients received appropriate antibiotic therapy. Highest temperatures, leukocyte counts, Pa(O(2))/FI(O(2)) ratios, and semiquantitative cultures of endotracheal aspirates were recorded from start of therapy until Day 14. Resolution was defined as the first day that these parameters fulfilled the following definition: temperature < or = 38 degrees C, leukocytes < or = 10 x 10(9)/L, Pa(O(2))/FI(O(2)) ratio > or = 25 kPa, and no or +1 of bacterial growth of etiologic pathogens in cultures of endotracheal aspirate. VAP was caused by Enterobacteriaceae (n = 14), P. aeruginosa (n = 7), S. aureus (n = 6), H. influenzae (n = 3), and S. pneumoniae (n = 1). H. influenzae and S. pneumoniae were eradicated from tracheal aspirates, whereas Enterobacteriaceae, S. aureus, and P. aeruginosa persisted, despite in vitro susceptibility to antibiotics administered. Significant improvements were observed for all clinical parameters, most apparently within the first 6 d after start of antibiotics. Newly acquired colonization, especially with P. aeruginosa and Enterobacteriaceae, occurred in the second week of therapy. Six patients developed a recurrent episode of VAP, four of them with P. aeruginosa. Clinical responses to therapy for VAP occur within the first 6 d of therapy, endotracheal colonization with Gram-negative bacteria persists despite susceptibility to therapy, and acquired colonization usually occurs in the second week of therapy and frequently precedes a recurrent episode.
Diphtheria Immunization of Adults
Authors: H. M. Leete
Journal: BMJ (1942)

SURGERY OF "AMPULLARY" CANCER MEDICALI OUNAL121termination only that part of the pancreas embraced by the concavity of the duodenum appeared to remain.No subse- quent sequela referable to the spleen made me regret the decision to conserve that organ, despite the surgical interference with both its arterial and its venous blood supply.The patient continued in excellent health until the autumn of 1938---that is, 11 years-when the possibility of a recurrence suggested itself.Just over 13 years after the removal of the tumour he died from undoubted abdominal malignancy-probably a late metastasis of the original tumour.Case 8: A Calcified Mass embedded in the Pancreas and com- pressing the Common Bile Duct; Removal of Mass together with Portion of Head of Pancreas.-Astoker 49 years of age with jaundice of 4 months' duration; rather poor physique.Two-stage operation after modern pre-operative measures.Cholecysto-gastrostomy; at operation a firm mass was palpated
NUTRITIONAL PROTECTION AGAINST SKIN DAMAGE FROM SUNLIGHT
Authors: Helmut Sies, Wilhelm Stahl
Journal: Annual Review of Nutrition (2004)

▪ Abstract The concept of systemic photoprotection by dietary means is gaining momentum. Skin is continuously exposed to ultraviolet (UV) radiation, the major cause of skin disorders such as sunburn, photodamage, and nonmelanoma skin cancer. Most of the erythemal annual UV dose is encountered under nonvacation conditions, when no sunscreen is applied. In the absence of topically added compounds, skin protection depends solely on endogenous defense. Micronutrients can act as UV absorbers, as antioxidants, or can modulate signaling pathways elicited upon UV exposure. UV-induced erythema is a suitable parameter to assess photoprotection. Dietary protection is provided by carotenoids, tocopherols, ascorbate, flavonoids, or n-3 fatty acids, contributing to maintenance resistance as part of lifelong protection.
A Genetic Snapshot of Small Cell Lung Cancer
Authors: Rafael Rosell, Luciano Wannesson
Journal: Cancer Discovery (2012)

Abstract Summary: Overexpression of PARP1 and enhancer of zeste homolog 2 (EZH2) has been identified in an integrated analysis of multiple proteins involved in intracellular signaling pathways in small cell lung cancer (SCLC) cell lines. The knockdown of PARP1 and EZH2 abrogated SCLC growth. These findings provide the basis for novel predictive markers and new treatment strategies in SCLC, for which there is now a paucity of available therapies. Cancer Discov; 2(9); 769–71. ©2012 AACR. Commentary on Byers et al., p. 798.
Inhibition of maturation and function of dendritic cells by intravenous immunoglobulin
Authors: Jagadeesh Bayry, Sébastien Lacroix-Desmazes, Cedric Carbonneil, Namita Misra, Vladimira Donkova, Anastas Pashov, Alain Chevailler, Luc Mouthon, Bernard Weill, Patrick Bruneval, Michel D. Kazatchkine, Srini V. Kaveri
Journal: Blood (2002)
Long-term follow-up of toluene diisocyanate-induced asthma
Authors: M. Padoan, V. Pozzato, M. Simoni, L. Zedda, G. Milan, I. Bononi, C. Piola, P. Maestrelli, P. Boschetto, C.E. Mapp
Journal: European Respiratory Journal (2003)

Eighty-seven cases of occupational asthma induced by toluene diisocyanate (TDI) were diagnosed by an inhalation challenge with TDI and methacholine. After an average follow-up interval of 11 yrs, all subjects were re-examined. Of the 87 subjects examined, 13 (15%) had remained in the same job, 44 (50.5%) had been removed from exposure for &lt;10 yrs and 30 (34.5%) had been removed for &gt;10 yrs. The proportion of subjects who experienced symptoms of asthma and those who were hyperresponsive to methacholine was significantly lower. Of the patients, 59% used short-acting bronchodilators, 8% long-acting bronchodilators and 18% were on regular inhaled glucocorticoids. Thus, multiple regression analysis showed a positive correlation between forced vital capacity (FVC) and forced expiratory volume in one second (FEV 1 ) at follow-up and FVC and FEV 1 at diagnosis, and a negative correlation with smoking and with therapy with bronchodilators. Stepwise logistic regression showed that the follow-up provocative dose causing a 20% fall in the FEV 1 (PD 20 ) could be predicted from baseline PD 20 . These results indicate that respiratory symptoms and airway hyperresponsiveness to methacholine persist in subjects removed from exposure to TDI for &gt;10 yrs. A more favourable prognosis was associated with a better lung function and a lower degree of airway hyperresponsiveness to methacholine at diagnosis.