tbuckley/cabot-search huggingface dataset:
FRI0118 Is the metabolic syndrome in rheumatoid arthritis patients influenced by the biologic therapy?
Authors: D. Nemes, E. Amaricai, L. Catan, M. Dragoi, D. Popa, G. Puenea, D. Andrei, R. Balacescu
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Metabolic syndrome (MetS) is a definition encompassing the risk factors for cardiovascular diseases (hypertension, obesity, diabetes mellitus and lipid profile disorders). Recent studies show that MetS frequency is higher in RA patients than in controls. Besides, among RA patients, MetS is associated with disease activity. <h3>Objectives</h3> To determine the frequency of Mets in RA patients and to compare the evolution of MetS parameters during the DMARD therapy versus during the combination of DMARDs and biologics. <h3>Methods</h3> 277 RA patients were investigated for the metabolic syndrome (MetS) defined according to the National Cholesterol Education Program. The first study group (40 patients) underwent DMARD therapy, while the second study group (48 patients) followed a combination of DMARDs and biologic therapy (TNF inhibitors or anti-CD20). The patients were assessed both for RA (CRP, DAS28-ESR) and MetS (triglycerides, HDL cholesterol and blood pressure) initially, after 1 year and after 2 years. <h3>Results</h3> 88 RA patients (31.7%) had also the criteria for MetS, 70 women (79.5%) and 18 men (20.5%). <h3>Conclusions</h3> The study showed that RA patients with MetS who followed a combination of DMARDs and biologic therapy presented a significant improvement of triglycerides and HDL-cholesterol in comparison to the RA patients who followed DMARD therapy both at 1-year and 2-years assessments. There were no differences of the blood pressure in the two groups. We found no statistically significant correlation among lipid levels, blood pressure and disease activity in either group. Further studies with a greater number of patients are required in order to establish a possible correlation. <h3>References</h3> da Cunha VR, Brenol CV, Brenol JC. Metabolic syndrome prevalence is increased in rheumatoid arthritis patients and is associated with disease activity. Scand J Rheumatol 2012;41(3):186-91 <h3>Disclosure of Interest</h3> None Declared
FRI0305 Higher frequency of metabolic syndrome in patients with hand osteoarthritis is more pronounced in OBESE patients:
Authors: C. Orellana, N. Navarro, J. Calvet, M. Garcia Manrique, J. Gratacόs, M. Larrosa
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Patients with knee osteoarthritis (OA), due to age, walking functional impairment and/or a high prevalence of obesity, are at a high risk of metabolic disorders and cardiovascular (CV) comorbidities. However, hand OA (HOA) usually affect a younger population and is not so clearly associated with obesity or other conditions linked to cardiovascular risk. <h3>Objectives</h3> To evaluate the frequency of metabolic disorders and CV comorbidity in patients with symptomatic HOA referred to a rheumatologist in a primary care setting. <h3>Methods</h3> Consecutive patients aged &gt;50 y referred period to a rheumatology practice in a primary care setting because of HOA during a 12-month period. Diagnostics related to CV comorbidity and/or glycemic or lipid disorders (hypertension, diabetes, ischemic heart disease, heart failure, cerebrovascular disorder, venous insufficiency or dyslipemia) were obtained for each patient from the HC3 (Història Clínica Compartida de Catalunya), a computerized database from the public health service. Patients with soft tissue disorders (e.g. shoulder tendinitis, plantar fasciitis, etc) adjusted for age were used as control group. <h3>Results</h3> Eighty-seven patients with HOA and 254 with soft tissue conditions were included in this cross-sectional study. Seventeen patients with HOA were excluded for the analysis because of concomitant knee and/or hip OA, leaving 70 patients with OA only in hands to be analyzed. No differences were found between both groups regarding age or gender. The frequencies of hypertension, hyperglycemia/diabetes and dyslipemia were not significantly different between HOA patients and the control group [Table]. However, obesity was significantly more frequent in the HOA group (13/70 (18.6%) vs. 23/254 (9.1%), p&lt;0.05), as was metabolic syndrome (9/70 (12.9%) vs. 13/254 (5.1%), p&lt;0.05). When analyzing the presence of metabolic syndrome among patients with obesity in both groups, patients with HOA still showed a significantly higher rate as compared with the control group (8/13 (61.5%) vs. 5/23 (21.7%), p&lt;0.05). [ <h3>Conclusions</h3> These data suggest that obesity and metabolic syndrome, but no other conditions associated with cardiovascular risk such as hypertension, diabetes or dyslipemia, are relatively frequent in patients with HOA. Metabolic syndrome was highly frequent in patients with HOA and obesity compared with obese control patients, suggesting the implication of additional systemic factors in this subpopulation. <h3>Disclosure of Interest</h3> None Declared
Gallium Scanning in the 'New' Tuberculosis
Authors: C. RICHARD GOLDFARB, CHARLOTTE COLP, FUKIAT ONGSENG, HOWARD FINESTONE, JEREMY HAVAS
Journal: Clinical Nuclear Medicine (1997)

Purpose: To evaluate the findings and usefulness of Ga-67 scanning in recent cases of tuberculosis (TB). Materials and Methods: The authors reviewed chest x-ray films and Ga-67 citrate scans of 52 patients with culture-confirmed infection caused by Mycobacterium tuberculosis treated after 1988. Results: Ga-67 scans were positive in every case, delineating upper lobe lung lesions in 6 patients, diffuse involvement or lower lobe disease in 34 patients, and intrathoracic adenopathy in 15 patients. Pulmonary parenchymal lesions were not detected on x-ray films in 3 patients, and nodal lesions were not apparent in 3 patients. In addition, in 6 patients cervical adenopathy was detected by Ga-67 scintigraphy; 4 underwent biopsy with culture confirmation. Conclusions: Ga-67 scanning is more sensitive than routine chest radiography for detection of both TB parenchymal lung involvement and adenopathy. Ga-67 imaging facilitates the choice of biopsy sites by identifying accessible peripheral nodes. Typical patterns in recent cases of TB differ significantly from the upper lobe predilection of classical TB.
Abstract 433: Role of AQP1 in invasion of GBM
Authors: Gay Samuelson, Burt Feuerstein, Anjan Misra, Kevin Bennett
Journal: Cancer Research (2010)

Abstract There has been little improvement in glioblastoma multiforme (GBM) patient prognosis over the past 20 years despite advances in imaging, neurosurgery, post-operative care, radiation delivery and chemotherapy. One reason for treatment failure is the highly invasive nature of glioma cells. Thus, it is essential to understand the mechanisms underlying invasion so that therapeutic targets can be developed and their impact assessed, non-invasively, during treatment. We have identified a trans-membrane protein, Aquaporin 1 (AQP1) whose over-expression correlates inversely with GBM survival (1,2) and drives migration of invading glioma cells (3). Our hypotheses are that the water permeation function of AQP1 drives migration and diffusion-weighted magnetic resonance imaging (DW-MRI) can be used to measure the water permeation and hence, invasion. The goal of our work is to determine the relationship between AQP1 expression and brain tumor invasion as measured in tissue culture by wound assay, in orthotopic murine brain slices by confocal microscopy and in rodent xenografts by DW-MRI. Modulating aquaporin channels alters water diffusion through cells and these changes have been detected with DW-MRI (4,5). We have data on 168 GBM patients that shows AQP1 overexpression correlates inversely with survival. Wound assay data indicates AQP1 overexpression drives migration and we have used an inhibitor of AQP1 water permeation, tetraethylammonium chloride (TEA) (6) to show that inhibiting water permeation also inhibits migration, We have histologic data on rodent xenografts demonstrating the aggressive invasive behavior of AQP1 overexpression compared to empty vector controls. We will present preliminary MRI results using an orthotopic rodent model where we modulate levels of AQP1 expression. References: 1. Verkman AS et al. J Mol Med (Epub), 2008. 2. Nagashima G et al. Brain Tumor Pathol 23(1), 2006. 3. Mirvish et al. Soc Neuro-Onc, 2007. 4. Latour LL. PNAS 91(4), 1994. 5. Thewall PE et al. Magn Reson Med 48(4), 2002. 6. Detmers et al. JBiolChem. 281(20); 14207, 2006. Supported by the Diane and Bruce Halle Fund, and the Barrow Neurological Foundation Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 433.
Abstract 2746: Genomic data mining for selecting informative markers for ancestry
Authors: Tesfaye Mersha Baye
Journal: Cancer Research (2011)

Abstract Given the huge amount of single nucleotide polymorphism (SNP) data available from high-throughput sources such as HapMap, data mining using various methods is a reasonable approach to identify SNPs that are informative for genetic ancestry. We extensively investigated the distribution and density of the SNPs across the genome of African and European populations available within the framework HapMap database to prioritize potential candidate SNPs useful for ancestry mapping in an admixed population. About 4 million SNPs were compared between Africans and Europeans using various measures of ancestry informativeness in use today viz. absolute allele frequency differences (Δ), Fisher Information Content (FIC), Shannon Information Content (SIC), informativeness for assignment (I), and Fixation Index (FST). Each method provides different sets of candidate ancestry informative markers (AIMs). The selected SNPs represent valuable resources for both controlling population structure and gene mapping studies. The overlap and non-overlap between selected AIMs by different measures of informativeness, data filtering strategies and the accuracy of each measure in classifying ancestral populations and applications in cancer genomics are discussed. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2746. doi:10.1158/1538-7445.AM2011-2746
Cox-2-derived PGE<sub>2</sub>induces Id1-dependent radiation resistance and self-renewal in experimental glioblastoma
Authors: Peter J. Cook, Rozario Thomas, Philip J. Kingsley, Fumiko Shimizu, David C. Montrose, Lawrence J. Marnett, Viviane S. Tabar, Andrew J. Dannenberg, Robert Benezra
Journal: Neuro-Oncology (2016)

In glioblastoma (GBM), Id1 serves as a functional marker for self-renewing cancer stem-like cells. We investigated the mechanism by which cyclooxygenase-2 (Cox-2)-derived prostaglandin E2 (PGE2) induces Id1 and increases GBM self-renewal and radiation resistance. Mouse and human GBM cells were stimulated with dimethyl-PGE2 (dmPGE2), a stabilized form of PGE2, to test for Id1 induction. To elucidate the signal transduction pathway governing the increase in Id1, a combination of short interfering RNA knockdown and small molecule inhibitors and activators of PGE2 signaling were used. Western blotting, quantitative real-time (qRT)-PCR, and chromatin immunoprecipitation assays were employed. Sphere formation and radiation resistance were measured in cultured primary cells. Immunohistochemical analyses were carried out to evaluate the Cox-2-Id1 axis in experimental GBM. In GBM cells, dmPGE2 stimulates the EP4 receptor leading to activation of ERK1/2 MAPK. This leads, in turn, to upregulation of the early growth response1 (Egr1) transcription factor and enhanced Id1 expression. Activation of this pathway increases self-renewal capacity and resistance to radiation-induced DNA damage, which are dependent on Id1. In GBM, Cox-2-derived PGE2 induces Id1 via EP4-dependent activation of MAPK signaling and the Egr1 transcription factor. PGE2-mediated induction of Id1 is required for optimal tumor cell self-renewal and radiation resistance. Collectively, these findings identify Id1 as a key mediator of PGE2-dependent modulation of radiation response and lend insight into the mechanisms underlying radiation resistance in GBM patients.
P1318 GENE ASSOCIATED WITH RETINOID-INTERFERON-β-INDUCED MORTALITY 19 SUPPRESSES HEPATITIS C VIRUS REPLICATION THROUGH THE IMPAIRMENT OF FATTY ACID BIOSYNTHESIS BY DOWNREGULATION OF ACETYL-CoA CARBOXYLASE
Authors: S.K. Yoon, J.-H. Kim, H. Wonhee, J.E. Choi, E.B. Lee, T.Z. Li, P.S. Sung, E.-C. Shin
Journal: Journal of Hepatology (2014)
PTH-071 Audit Of Subsequent Outcome In Patients Admitted To Hospital With Alcohol Use Disorder (aud)
Authors: S Rai, H Boyce, F Anderson, D Gleeson
Journal: Gut (2014)

<h3>Introduction</h3> There are limited published data on subsequent outcome of patients admitted acutely to hospital with alcohol use disorders (AUDs), in regard to drinking relapse, hospital readmission and death. <h3>Methods</h3> We conducted a prospective audit of 142 patients (105 men), aged (median (range) 46 (23–78) years) admitted with AUDs to a medical admission unit during Oct-Nov 2012 and Jan-March 2013. Information on hospital readmission, AandE attendance and death (from hospital electronic records), and on subsequent alcohol drinking (from records and from telephoning patients) was gathered up to 21/05/13. Data were analysed by life-table and Cox regression analysis. <h3>Results</h3> Of the 142 patients, 80 (56%) lived alone and 121 (85%) were unemployed. 36 patients (25%) had liver disease (Child-Pugh Grade B/C). Of 92 patients with CT or MRI brain scan within 5 years, 49 (53%) had brain atrophy. 73 patients (51%) had another mental health problem (anxiety or depression in 68, schizophrenia in 5). Over the previous year, 71 (50%) had &gt;1 previous AUD-related admission, and 24 (17%) had &gt;3 such admissions. Out of 110 patients, 79% of patients said they intended to stop drinking. Length of stay during index admission was 6 (0–61 days). 51 patients experienced complications, 29 self-discharged early and 18 were verbally ± physically abusive. 5 patients died during admission, 4 from liver disease. 17 discharged patients were lost to follow up; of the remaining 120, 96 relapsed into drinking, 18 (0–168 days) after discharge. 100-day relapse rate was 78%. When asked the reason for relapse (n = 87), 53 patients cited “no particular reason”, 22 depression, 5 a traumatic experience and 4 a celebratory event. Relapse was independently associated with self-discharge after index admission (p &lt; 0.001). 77 patients (56%) were readmitted to hospital, 66 (86%) for clearly alcohol-related reasons. 13 more patients re-attended the AandE Department without readmission. 100 day readmission rate was 50%. 19 patients were readmitted twice and 23 patients &gt;3 times. Readmission was independently associated with unemployment (p = 0.043), self-discharge after index admission (p = 0.011), relapse into drinking (p = 0.028), and (surprisingly) with having received a brief intervention regarding alcohol consumption during the index admission from a dedicated alcohol worker (n = 61, p = 0.009). Seven more patients had died by 21/05/13, 5 from liver disease. <h3>Conclusion</h3> Patients admitted to hospital with AUDs tend to be socially deprived, frequent hospital attenders with major physical and mental co-morbidity. They have high subsequent alcohol relapse and hospital readmission rates. Reduction of these is not achieved by interventions during the index admission and will require more pro-active measures post-discharge. <h3>Disclosure of Interest</h3> None Declared.
THE ASSOCIATION BETWEEN PROGRESSIVE PERI-STENT CONTRAST STAINING, MAJOR ADVERSE CARDIAC EVENTS, AND VERY LATE STENT THROMBOSIS IN PATIENTS AFTER STENT IMPLANTATION
Authors: Takeshi Tada, Kazushige Kadota, Daiji Hasegawa, Seiji Habara, Hiroyuki Tanaka, Yasushi Fuku, Tsuyoshi Goto, Kazuaki Mitsudo
Journal: Journal of the American College of Cardiology (2015)
Biochemical Studies of Erythrocytes in a Patient with Pyroglutamic Acidemia (5-Oxoprolinemia)
Authors: Sverre Marstein, Egil Jellum, Bert Halpern, Lorentz Eldjarn, Thomas L. Perry
Journal: New England Journal of Medicine (1976)

An adult with pyroglutamic acidemia was investigated biochemically in efforts to explain his overproduction of pyroglutamate and his neurologic disease. The patient's erythrocytes contained no detectable glutathione, and their glutathione synthetase activity was less than 2 per cent of normal. These cells were loaded with free amino acids, most of them in concentrations five to 100 times normal. The erythrocytes also contained the unusual compound methionine sulfoxide. In skeletal muscle, in which glutathione was only reduced to 3 per cent of normal, there was no amino acid accumulation. The patient's overproduction of pyroglutamate is probably caused by increased in vivo activity of γ-glutamyl-cysteine synthetase due to absence of normal feedback inhibition by glutathione, with a resulting abundance of substrates available for γ-glutamyl cyclotransferase. Although lack of glutathione is apparently tolerable in short-lived erythrocytes, it could be disastrous in non-renewable neurons. Glutathione deficiency in brain may explain the patient's neurologic deterioration. (N Engl J Med 295:406–412, 1976)
Increased microRNA-323-3p in IL-22/IL-17-producing T cells and asthma: a role in the regulation of the TGF-β pathway and IL-22 production
Authors: J. Kärner, M. Wawrzyniak, S. Tankov, T. Runnel, A. Aints, K. Kisand, A. Altraja, K. Kingo, C. A. Akdis, M. Akdis, A. Rebane
Journal: Allergy (2016)

IL-22- and IL-17-producing T cells have important roles in allergic diseases. MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression and modulate numerous biological processes. Little is known about the functions of miRNAs in IL-22/IL-17-producing T cells.IL-22- and IL-17-positive T cells were sorted from human peripheral blood mononuclear cells (PBMCs) by intracellular staining and dual-secretion assay. miRNA expression profiles were detected with TaqMan array microfluidic cards. T cells were transfected with miRNA mimics. Gene expression was analyzed using RT-qPCR and/or enzyme-linked immunosorbent assay in T-cell subsets and PBMCs from patients with asthma and atopic dermatitis.The increased expression of miR-323-3p and noncoding RNA nc886 and reduced expression of miR-93, miR-181a, miR-26a, and miR-874 were detected in IL-22-producing T cells. The pathway analysis of the putative targets suggested that these differentially expressed miRNAs could impact the proliferation, differentiation, and effector functions of T cells. Further analyses showed the highest expression for miR-323-3p in IL-22- and IL-17-double-positive T cells and its capacity to suppress multiple genes from the transforming growth factor-β pathway and the production of IL-22 in T cells. An increased expression of miR-323-3p in PBMCs from patients with asthma and reverse correlation between miR-323-3p levels and IL-22 production in PBMCs cultured in T-cell growth conditions was observed.Our data suggest that miR-323-3p acts in a negative feedback loop to control the production of IL-22 in IL-22/IL-17-producing T cells and might thus impact the T-cell responses in asthma.
LIFETIME MARIJUANA USE IS ASSOCIATED WITH INCREASED RISK OF ABDOMINAL AORTIC-ILIAC CALCIUM AND NOT CORONARY ARTERY CALCIUM BY MIDDLE AGE
Authors: Jamal S. Rana, Reto Auer, Eric Vittinghoff, Mark Pletcher, Norrina Allen, Jared Reis, Cora Lewis, Jeffrey Carr, David Goff, Stephen Sidney
Journal: Journal of the American College of Cardiology (2016)
Clinical Significance of Minimal Residual Disease in Childhood Acute Lymphoblastic Leukemia
Authors: Hélène Cavé, Jutte van der Werff ten Bosch, Stefan Suciu, Christine Guidal, Christine Waterkeyn, Jacques Otten, Marleen Bakkus, Kris Thielemans, Bernard Grandchamp, Etienne Vilmer, Brigitte Nelken, Martine Fournier, Patrick Boutard, Emmanuel Lebrun, Françoise Méchinaud, Richard Garand, Alain Robert, Nicole Dastugue, Emmanuel Plouvier, Evelyne Racadot, Alice Ferster, Jan Gyselinck, Odile Fenneteau, Michel Duval, Gabriel Solbu, Anne-Marie Manel
Journal: New England Journal of Medicine (1998)

The implications of the detection of residual disease after treatment of acute lymphoblastic leukemia (ALL) are unclear. We conducted a prospective study at 11 centers to determine the predictive value of the presence or absence of detectable residual disease at several points in time during the first six months after complete remission of childhood ALL had been induced. Junctional sequences of T-cell–receptor or immunoglobulin gene rearrangements were used as clonal markers of leukemic cells. Residual disease was quantitated with a competitive polymerase-chain-reaction (PCR) assay. Of 246 patients enrolled at diagnosis and treated with a uniform chemotherapy protocol, 178 were monitored for residual disease with one clone-specific probe (in 74 percent) or more than one probe (in 26 percent). The median follow-up period was 38 months.
Ipilimumab-Induced Hepatitis on 18F-FDG PET/CT in a Patient With Malignant Melanoma
Authors: Roy A Raad, Anna Pavlick, Rajni Kannan, Kent P Friedman
Journal: Clinical Nuclear Medicine (2014)

A 78-year-old man with metastatic malignant melanoma underwent a restaging 18F-FDG PET/CT after initiation of ipilimumab therapy, a Food and Drug Administration–approved human monoclonal antibody targeting CTLA-4. PET/CT demonstrated intense FDG uptake fusing to poorly circumscribed hypodensities throughout the liver. Patient was experiencing high-grade fever, chills, and generalized fatigue at the time of imaging, as well as mildly elevated liver function tests. Patient was subsequently treated with corticosteroids for suspected ipilimumab-induced hepatitis, and the patient rapidly improved clinically. Follow-up PET/CT 2 months later revealed complete resolution of abnormal FDG uptake in the liver, confirming the diagnosis of ipilimumab-induced hepatitis.