CLINICAL APPLICATION OF THE THIOCHROME REACTION IN THE STUDY OF THIAMIN (VITAMIN B^1) DEFICIENCY
Authors: Harry J. Borsox
Journal: Annals of Internal Medicine (1940)

Article1 July 1940CLINICAL APPLICATION OF THE THIOCHROME REACTION IN THE STUDY OF THIAMIN (VITAMIN B1) DEFICIENCYHARRY J. BORSOX, M.D.HARRY J. BORSOX, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-14-1-1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptA Chemical method for the estimation of thiamin (vitamin B1), should have the advantages, as compared to biological assay, of speed, accuracy, cheapness, and ease of performance. The great advantage of biological assay is definite specificity. However, under well controlled conditions the uncertainties of chemical assay may become negligible.Of the several methods proposed for the chemical estimation of thiamin, the thiochrome method of Jansen1has received the most attention. Recently, the method of Prebluda and McCollum2as modified by Melnick and Field3has been shown to possess a high degree of specificity and accuracy, but the technic as described...References1. JANSEN BC: A chemical determination of aneurin (vitamin B1) by the thiochrome method, Recueil trav. chim. Pays Bas, 1936, lv, 1046-1052. CrossrefGoogle Scholar2. PREBLUDAMCCOLLUM HJEV: Chemical reagent for thiamin, Jr. Biol. Chem., 1939, cxxvii, 495-503. CrossrefGoogle Scholar3. MELNICKFIELD DH: Chemical determination of vitamin B1; reaction between thiamin in pure aqueous solution and diazotized p-aminoacetophenone, Jr. Biol. Chem., 1939, cxxvii, 505-514. CrossrefGoogle Scholar4. LIGHTSCHULTZATKINCRACAS RFASLLJ: Excretion of vitamin B1 in urine and feces, Jr. Nutr., 1938, xvi, 333-341. CrossrefGoogle Scholar5. SCHOPFER WH: La spécificité d'action de l'aneurine sur Phycomyces. Le röle des constituants de l'aneurine et de leurs produits de substitution, Bull. Soc. bot. Suisse, 1937, xlvii, 460-464. Google Scholar6. MEIKLEJOHN AP: Estimation of vitamin B1 in blood by modification of Schopfer's test, Biochem. Jr., 1937, xxxi, 1441-1451. CrossrefGoogle Scholar7. BONNERERICKSON JJ: The Phycomyces assay for thiamin (vitamin B1): The method and its chemical specificity, Am. Jr. Bot., 1938, xxv, 685-692. CrossrefGoogle Scholar8. ROWLANDSWILKINSON ENJF: Clinical significance and estimation of blood vitamin B1 , Brit. Med. Jr., 1938, ii, 878-883. CrossrefGoogle Scholar9. HARRISLEONGUNGLEY LJPCCC: Measurement of vitamin B1 in human urine as index of nutritional level; further quantitative data, Lancet, 1938, i, 539-545. CrossrefGoogle Scholar10. WESTENBRINKGOUDSMIT HGJ: Determination of aneurin (vitamin B1) in urine by the thiochrome reaction, Recueil trav. chim. Pays Bas, 1937, lvi, 803-810. Een chemische methode voor de bepaling van het aneurine (vitamin B1) in urine, Nederl. Tijdschr. v. Geneesk., 1937, lxxxi, 2632-2639. Google Scholar11. WESTENBRINKGOUDSMIT HGJ: Het verband tusschen opneming en uitscheiding van het aneurine (vitamin B1) door den gezonden mensch, Nederl. Tijdschr. v. Geneesk., 1937, lxxxi, 4056-4062. Verdere onderzoekingen over het verband tusschen opneming en uitscheiding van aneurine door den mensch, ibid., 1938, lxxxii, 518-527. Aneurinestofwisseling en zwangerschap, ibid., 1938, lxxxxii, 1076-1083. On relation of uptake and excretion of aneurin (vitamin B1) in cases of normal subjects, Arch, néerlandaise de physiol., 1937, xxii, 319-325. Observations on relation between intake and excretion of aneurin in cases of normal subjects and pregnant women, ibid., 1938, xxiii, 79-96. Google Scholar12. KARRERKUBLI WU: Zur Bestimmung von Vitamin B1 , Helvetica chim. acta, 1937, xx, 369-373. KARRER, W.: Zur Bestimmung von Vitamin B1 im menschlichen Harn, ibid., 1937, xx, 1147-1155. CrossrefGoogle Scholar13. PYKE MA: Chemical measurement of vitamin B1 in foodstuffs and biological material by means of the thiochrome reaction, Biochem. Jr., 1937, xxxi, 1958-1963; Nature, 1938, cxli, 1141. CrossrefGoogle Scholar14. RITSERT K: Über die Ausscheidung von peroral und parenteral zugeführten Aneurin, Klin. Wchnschr., 1938, xvii, 1397-1400. Zur Aneurinbestimmung im Harn nach der Jansenschen Thiochrommethode, Deutsch, med. Wchnschr., 1938, lxiv, 481-484. CrossrefGoogle Scholar15. CLINEJOLLIFFE JKN: Cited by WILLIAMS, R. R., and SPIES, T. D., Vitamin B1 (thiamin) and its use in medicine, 1938, The MacMillan Co., New York. Google Scholar16. WESTENBRINKGOUDSMIT HGJ: Investigations on the aneurin and cocarboxylase content of animal tissues, estimated by the thiochrome method, Enzymologia, 1938, v, 307-320. Google Scholar17. WATSON CJ: Urinary pigments in four cases of alcoholic pellagra, Proc. Soc. Exper. Biol., and Med., 1938, xxxix, 514-518. CrossrefGoogle Scholar18. HENNESSYCERECEDO DJLR: The determination of free and phosphorylated thiamin by a modified thiochrome assay, Jr. Am. Chem. Soc., 1939, lxi, 179-183. CrossrefGoogle Scholar19. BORSOOK H: To be published. Google Scholar20. COWGILL G: The vitamin B requirement of man, 1934, Yale University Press, New Haven. Google Scholar21. WILLIAMSSPIES RRTD: Vitamin B1 (thiamin) and its use in medicine, 1938, The MacMillan Co., New York. Google Scholar22. COWGILLROSENBERGROGOFF GHJ: Studies in physiology of vitamins; XIV. The effect of administration of large amounts of water on the time required for the development of the anorexia characteristic of a deficiency of the vitamin B complex, Am. Jr. Physiol., 1930, xcv, 537-541. CrossrefGoogle Scholar23. VAN DER SCHEERKOEK WMHC: Peripheral nerve lesions in cases of pernicious anemia, Acta psych, et neur., 1938, xiii, 61-92. CrossrefGoogle Scholar24. REESEHODGSON HHER: Tabes dorsalis and vitamin B deficiency, Urol. and Cutan. Rev., 1939, xliii, 56-58. Google Scholar25. BORSOOKKREMERSWIGGINS HMYCG: The relief of symptoms in major trigeminal neuralgia following the administration of massive doses of vitamin B1 , Science, 1939, lxxxix, 439. Google Scholar26. DANNCOWGILL MG: The influence of diarrhea on the vitamin B1 requirement, Arch. Int. Med., 1938, lxii, 137-150. CrossrefGoogle Scholar27. CHENEY G: Vitamin B1 and liver extract in the treatment of non-specific chronic diarrhea and colitis, Am. Jr. Digest. Dis., 1939, vi, 161-169. CrossrefGoogle Scholar28. JOLLIFFEGOODHARTGENNISCLINE NRJJK: The experimental production of vitamin B1 deficiency in normal subjects; dependence of urinary excretion of thiamin on dietary intake of vitamin B1 , Am. Jr. Med. Sci., 1939, cxcviii, 198-211. CrossrefGoogle Scholar29. OCHOAPETERS SRA: Vitamin B1 and cocarboxylase in animal tissues, Biochem. Jr., 1938, xxxii, 1501-1515. CrossrefGoogle Scholar30. GOODHARTSINCLAIR RSHM: The estimation of cocarboxylase (vitamin B1 diphosphate ester) in blood, Biochem. Jr., 1939, xxxiii, 1099-1108. CrossrefGoogle Scholar31. WANGHARRIS YLLJ: Methods for assessing the level of nutrition of the human subject. Estimation of vitamin B1 in urine by the thiochrome test, Biochem. Jr., 1939, xxxiii, 1356-1369. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: San Francisco, California*Received for publication November 18, 1939.From the Divisions of Medicine and Pathology, University of California Medical School, San Francisco.Supported by a grant from the Christine Breon Fund for Medical Research.The synthetic thiamin chloride used in this study was furnished by E. R. Squibb and Sons. 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