tbuckley/cabot-search huggingface dataset:
Abstract 1061: EZH2 expression in different subtypes of human breast cancer
Authors: Galina F. Khramtsova, Abul B.M.M.K. Islam, Andrey I. Khramtsov, Phoebe Lyman, Liao Chuanhong, Alba Jene-Sanz, Walmy E. Sveen, Dezheng Huo, Nuria Lopez-Bigas, Elizaveta V. Benevolenskaya, Olufunmilayo I. Olopade
Journal: Cancer Research (2012)

Abstract Introduction: Cancer is characterized by aberrant patterns of expression of multiple genes believed to be due to not only genetic but also epigenetic changes. The epigenetic changes are communicated through chemical modifications, including modifications of histone proteins. For each histone, multiple histone-modifying enzymes have been discovered in mammals. We found that the expression of genes with histone modifications was highly predictable from the level of expression of the corresponding histone-modifying enzyme. Specifically, expression of genes displaying histone H3 K27 methylation was dependent on EZH2 expression. Method: We analyzed breast cancer tissue arrays for EZH2 expression. Tissue microarrays were constructed from 95 histologically confirmed breast cancer samples. To identify breast cancer subtypes, the tissues were evaluated for the expression of ER, PR, HER2, CK5/6 and EGFR. Staining with vimentin served as a control to monitor the quality of tissue fixation in archival tumors. Breast cancer subtypes were defined as luminal A (ER+ and/or PR+, HER2-), luminal B (ER+ and/or PR+, HER2+), basal-like (ER-, PR-, HER2-, CK5/6+, and/or EGFR+), HER2+/ER- (HER2+, ER-, PR-), and unclassified (negative for all five markers). EZH2 expression was measured by staining using an anti- EZH2 antibody (Invitrogen, cat. #18-7395). Staining quantification was performed independently by two pathologists. Results: The mean age of the patients was 56 years old (SD=15.6). 63.7% of the patients had estrogen receptor positive tumors, 50.6% had progesterone receptor positive tumors, and 21.2% had HER2+ tumors. The distribution of breast cancer subtypes is luminal A (58.9%), luminal B (6.3%), HER2+/ER- (4.2%), basal-like (28.5%), unclassified (2.1%). A majority of the patients had high grade tumors (46.0% grade II and 47.1% grade III). High EZH2 expression was associated with Her2+ and basal-like tumors (P&amp;lt;0.0001). Conclusion: In this cohort, high expression of EZH2 was associated with the most aggressive breast cancer phenotypes and likely reflects increased expression of histone modifying proteins in the tumors. Supported by Grants: NIH R01CA138631 (E.V.B.), R01 CA142996 and P50 CA125183 (O.I.O). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1061. doi:1538-7445.AM2012-1061
SP0139 Predictors for Outcome of Juvenile SLE
Authors: T. Avcin
Journal: Annals of the Rheumatic Diseases (2013)

<h3></h3> Approximately 20% of patients withy systemic lupus erythematosus (SLE) have disease which begins in childhood. Several series have reported that childhood-onset SLE (cSLE) is often more severe than adult disease with more pronounced clinical, immunologic and serologic abnormalities. In particular, renal and central nervous system (CNS) involvement tend to be more severe in pediatric than in adult patients. The morbidity and mortality associated with SLE result from a combination of severe organ damage from disease, infection or side effects and complications of treatment. Since neither disease activity nor disease damage in SLE can be measured directly with any clinical sign or laboratory value, a number of disease indices have been developed that allow standardized comparison of SLE cohorts. Disease activity indices including SLEDAI, SLAM, BILAG and ECLAM have been validated in cSLE and none appears superior. The SLICC damage index was developed to measure permanent disease damage in adults with SLE and was subsequently validated in cSLE. It has been suggested that pediatric version should include additional domains addressing pediatric specific issues such as growth failure and delayed puberty, but both indices are reversible and they do not fulfil the definition of nonreversible organ damage. Several predictors (risk factors) have been linked with poor prognosis in cSLE. Using the SLICC-DI scoring system for damage, it has been demonstrated that renal and/or CNS disease represent major determinants of morbidity and mortality in cSLE. Diffuse proliferative glomerulonephritis is most consistently linked with a poor prognosis if associated with hypertension. Other suggested risk factors associated with poor prognosis in cSLE include acute thrombocytopenia, young age at diagnosis, male sex and nonwhite (black, Asian and Hispanic) ethnicity. The presence of antiphospholipid antibodies may predispose to disease damage by increasing the risk for recurrent venous thrombosis, stroke and thrombocytopenia. Similar to previous adult studies, it has been shown in cSLE that cumulative disease activity over time results in increased damage, and early introduction of therapy with immunosuppressive drugs might improve the outcome by preventing permanent damage. The exact impact of corticosteroid therapy on increased disease damage is still controversial, but patients may benefit from judicious use of corticosteroid with refined tapering schedules and early introduction of steroid-sparing drugs. It has been also demonstrated that frequent occurrence of severe, major disease flares requiring intravenous cyclophosphamide treatment represent a risk factor for damage, but not mild or moderate flares. <h3>Disclosure of Interest</h3> None Declared
Abstract LB-105: Identifying molecular markers associated with chemo-resistance in ovarian cancer stem cells
Authors: Sheetal Dyall, Kate Lawrenson, Dimitra Dafou, Simon A. Gayther
Journal: Cancer Research (2011)

Abstract Stem-like cancer cells, or cancer stem cells (CSC) have been proposed to sustain the growth of tumour cells and give rise to drug resistance in cancer. Side populations (SP) of cells, characterised by their differential ability to efflux Hoechst 33342 from their cytoplasm via multi-drug transporters, are also thought to be enriched in CSCs. The aim of this study was to identify SPs of cancer cells in epithelial ovarian cancer (EOC), and genes within SP populations that both characterize SP cell in EOC cell lines and play a role in the development of resistance to chemotherapeutic agents. Using flow cytometry, we isolated SP cells from a panel of EOC cell lines. Detectable levels of viable SP cells were observed in the cell lines IGROV1 (1.8–5%), OVCAR3 (0.8%), OVCAR8 (0.2%), and the matched pairs of cisplatin-sensitive cell lines and their resistant variants OV2008 (2.5%)/C13*(1.5%) and A2780 (0.2%)/A2780CP (0.2%). In vitro characterization of SP cells from IGROV1, OVCAR3 and OVCAR8, compared to the non-SP cells showed that SP cells have higher colony forming efficiency when cultured as three-dimensional spheroids in non-adherent culture conditions. A proportion of single SP cells also had the ability to form multi-cellular spheroids in culture (a stem-cell ‘like’ feature) while non-SP cells did not. Under adherent culture conditions, SP cells show a range of morphologies, consisting of holoclones (which are enriched in SP cells) and paraclones (depleted of SP cells). Sub-cloning of holoclones generated both holoclones and paraclones; culturing paraclones led to the formation of only a few paraclones, which senesced. Differential whole-genome gene expression microarray profiling of SP vs non-SP cells identified the P-glycoprotein ABCB1 as the most highly upregulated gene in SP cells, consistent with other studies. Profiling of A2780 and its chemo-resistant derivative A2780CP identified Lin28, an embryonic stem cell marker, as the most differentially over-expressed gene in A2780CP SP cells. SiRNAs to ABCB1 were used to test the hypothesis that knockdown of this marker reduce proportionally the stem cell population in the IGROV1 EOC cell line; siRNAs to Lin28 were used to test whether knockdown of this gene re-sensitized A2780CP cells to cisplatin. Ongoing studies are evaluating the effects on chemosensitivity in vivo, by knocking down Lin28 in A2780CP cells expressing a luciferase reporter. Using Xenogen live animal imaging technologies, tumor burden is being evaluated in real-time, comparing Lin28 knockdown xenografted cell lines with non-silencing siRNA controls. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-105. doi:10.1158/1538-7445.AM2011-LB-105
Fresh Fruit Consumption and Major Cardiovascular Disease in China
Authors: Huaidong Du, Liming Li, Derrick Bennett, Yu Guo, Timothy J. Key, Zheng Bian, Paul Sherliker, Haiyan Gao, Yiping Chen, Ling Yang, Junshi Chen, Shanqing Wang, Ranran Du, Hua Su, Rory Collins, Richard Peto, Zhengming Chen
Journal: New England Journal of Medicine (2016)

In Western populations, a higher level of fruit consumption has been associated with a lower risk of cardiovascular disease, but little is known about such associations in China, where the consumption level is low and rates of stroke are high.
MITRAL VALVE REPLACEMENT IMPAIRS LEFT VENTRICULAR BLOOD FLOW
Authors: Koichi Akiyama, Keiichi Itatani, Mao Kinoshita, Masaru Shimizu, Saeko Hamaoka, Hideya Kato, Yoshifumi Naito, Yasufumi Nakajima, Satoshi Numata, Toshiki Mizobe, Hitoshi Yaku, Teiji Sawa
Journal: Journal of the American College of Cardiology (2016)
Drug Therapy in Hypertension with Hemorrhagic Hypertensive Retinitis
Authors: Clem F. Burnett, James A. Evans
Journal: New England Journal of Medicine (1955)

THE purpose of this paper is to add our experience with the newer hypotensive drugs in the treatment of patients with severe hypertension and hemorrhagic eye grounds to the ever-growing list of reports on this subject. This treatment is too new to justify any final conclusions, but, in our short experience, it does appear to offer more than any previous medical therapy in this devastating disease.Since hypertension can be a benign disease with no effect on longevity, or a rapidly progressing, fatal disease, it is difficut to evaluate the effects of therapy on the final outcome. Keith, Wagener and . . .
Abstract 2248: Oncogenic actions of HDGF (hepatoma-derived growth factor) in colorectal cancer: Implication for tumor biology/ patient prognosis and modulation by butyrate
Authors: Theodor Kudlich, Maike Wilk, Tilmann Neun, Elena Hartmann, Dorothee Rogoll, Hardi Luehrs, Andreas Rosenwald, Tiemo Katzenberger, Wolfgang Scheppach, Michael Scheurlen, Ralph Melcher
Journal: Cancer Research (2011)

Abstract HDGF was identified as an oncogene relevant for colon cancer carcinogenesis by cDNA-array analysis, RT-PCR and immunohistochemistry. The analysis were performed in colonic adenoma (Geki2), carcinoma (HT29) and metastasis (SW620) cell lines, and in 50 colorectal cancer tissues and corresponding normal mucosa. By incubation of the cell lines with the short chain fatty acid butyrate (4mM), it was possible to (down-) regulate the HDGF-expression level. In the patients we analysed, HDGF overexpression was not associated with a worse prognosis or any other clinical parameter. To study the mechanisms of action of HDGF we stably transfected a HDGF-siRNA containing plasmid into HT29 colon carcinoma cells, resulting in a almost complete inactivation of HDGF-expression. This blocking leads to altered growth characteristics and cell differentiation and the cells exhibit potent pro-apoptotic properties. An affymetrix-expression array was performed and first results will me shown at the meeting. In conclusion, HDGF might be a potential therapeutic target for human colorectal cancer and a modulation of expression is possible by the short chain fatty acid butyrate. These findings may have major implications in the treatment of colorectal cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2248. doi:10.1158/1538-7445.AM2011-2248
Renal Impairment Due to Sarcoid Infiltration of the Kidney
Authors: Kenneth W. Berger, Arnold S. Relman
Journal: New England Journal of Medicine (1955)

IT is well known that sarcoid can involve many organs. At autopsy it is not rare to find infiltration of the kidneys,1 but this is usually quite minimal in degree. Clinical renal insufficiency may occur in patients with sarcoidosis, but it is usually due to hypercalcemia and nephrocalcinosis2 and not to involvement of the kidneys by the sarcoid process itself. There have been, in addition, a few reports of patients with sarcoidosis and renal failure in whom the renal lesion proved to be the result of other, probably unrelated processes. This report concerns a patient with generalized sarcoidosis and clinical . . .
SAT0365 Evaluation of bone status in algerian epileptic patients
Authors: M. Djennane, S. Slimani
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Osteoporosis and epilepsy are two common affections, mostly diagnosed in the elderly. The prevalence of epilepsy is 1% in people over 60 years and increases with age. An increased risk of fracture has been reported in patients with epilepsy when compared to the general population. The aim of our study was to assess the bone status in epileptic patients and to identify risk factors for osteoporosis. <h3>Methods</h3> Eighty-two patients with epilepsy (26 M; 56F) and 98 controls matched for age and sex were included. For each patient, the following data were collected: risk factors for osteoporosis, serum dosage of 25-OH D, estimation of calcium intake by the <i>Fardelonne</i> questionnaire, radiological assessment including <i>vertebral fracture assessment</i> (VFA) and bone densitometry DXA (Hologic QDR). <h3>Results</h3> The mean age of patients was 47.0±17.2 years (15 – 74), mean disease duration 13.6±7.54 years, mean duration since therapy was started 10.2±5.51 years (2 – 39), mean BMI 25,1±3,2 kg/m<sup>2</sup> (15,4 - 36,9), mean calcium intake 736±148 mg/d (427 - 1131) in cases and 868±125 in controls (<i>p</i>=0.037). The mean level of 25-OH D was 17,6±6,7 ng/L (6 - 31) in cases and 19.4±5.8 ng/L in controls (<i>p</i>=0.002). Vitamin D deficiency (&lt;20 ng/L) was noted in 72.0% cases and 48.0% controls. The number of vertebral fractures was 11 in cases and 4 in controls (<i>p</i>=0.003). Smoking concerned 22% cases and 78% controls and alcohol intake 18.3% cases and 82.7% controls. The mean vertebral T-score was -1.91±2.06 in cases Vs 0.59±1,48 in controls (<i>p</i>&lt;0.001); the mean femoral T-score was -1.16±1.23 in cases Vs -0.45±1.02 in controls (<i>p</i>&lt;0.001). Based on the IOF definition, 38.8% cases and 43.9% controls were osteopenic. Cases had lower vertebral and femoral BMD values compared with controls. Identified risk factors for a lower BMD were female, advanced age, low calcium intake and lower levels of 25-OH D. Finally, there was a significant correlation between BMD and a higher BMI, a younger age, a higher calcium intake, a higher level of 25-OH D and the number of fractures. <h3>Conclusions</h3> Osteoporosis is more frequent in epileptic patients taking antiepileptic medications than in the general population. Risk factors are female, age, low calcium intake and low levels of 25-OH D. Detection of osteoporosis in this subpopulation seems interesting as well as the supplementation of patients undertaking antiepileptic medications with vitamin D. <h3>Disclosure of Interest</h3> None Declared
Progressive Transformation of Germinal Centers in a Pediatric Patient
Authors: William Makis, Anthony Ciarallo, Javier-A. Novales-Diaz, Robert Lisbona
Journal: Clinical Nuclear Medicine (2011)

Progressive transformation of germinal centers (PTGC) is a benign reactive adenopathy that leads to abnormal nodal enlargement. Although not believed to be premalignant, recent genetic studies have found evidence linking PTGC to Hodgkin lymphoma. In this case of a 14-year-old girl referred for an F-18 FDG PET/CT, extensive FDG-avid lymphadenopathy that was suspicious for lymphoma, was confirmed to be PTGC on biopsy. A follow-up PET/CT showed significant resolution of FDG-avid disease. As a new understanding of a possible link between PTGC and Hodgkin lymphoma emerges, serial PET/CT imaging may play a prominent role in the staging and follow-up of PTGC.
Abstract 1728: Bcl-XL overexpression prevents B Cell receptor driven autophagy in IgM+ lymphoma.
Authors: Gregory B. Carey, Sanjit K. Roy, Alphius Sesay
Journal: Cancer Research (2013)

Abstract Our previous studies showed that B cell receptor (BCR) induced apoptosis in lymphoma was preceded by suppression of PI-3K [Carey GB, Scott DW (2001) J. Immunol, 166: 1618-1626]. PI-3K stimulates mTOR, a suppressor of autophagy and mitophagy. In addition, Bcl-XL is known to inhibit BCR-driven apoptosis in lymphoma, although the underlying mechanism is not clear. Therefore the present study examined autophagy in BCR induced apoptosis in lymphoma cells expressing high or low levels of Bcl-XL. WEHI-231 and WEHI-231 cells expressing Bcl-XL (WEHI-231/Bcl-XL) were treated with anti-IgM to cross-link the BCR and to determine the cellular processes leading to apoptosis. The results confirmed dramatic mitochondrial dysfunction followed by apoptosis in parental WEHI-231 compared to WEHI-231/BclXL. BCR-crosslinking resulted in a massive and rapid induction of the autophagic response in parental WEHI-231 compared to those expressing Bcl-XL. Furthermore, treatment with STF-62247, a small molecule inducer of autophagy, resulted in apoptosis in WEHI-231 but not WEHI-231/BclXL cells. These data suggest that BclXL prevents BCR-driven apoptosis in lymphoma via blocking autophagic and mitophagic responses. Hence, combinations of small molecule autophagy inducers and Bcl-XL antagonists may prove to be effective in addressing lymphoma. Supported by NCI Grant UH2CA158689 to G.B.C. Citation Format: Gregory B. Carey, Sanjit K. Roy, Alphius Sesay. Bcl-XL overexpression prevents B Cell receptor driven autophagy in IgM+ lymphoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1728. doi:10.1158/1538-7445.AM2013-1728
AB0546 The prevalence of inflammatory back pain in a uk primary care population
Authors: L. E. Hamilton, A. Macgregor, V. Warmington, E. Pinch, K. Gaffney
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Inflammatory back pain (IBP) is the earliest and commonest symptom of axial spondyloarthropathy (SpA). SpA can be difficult to diagnose in its early stages, but the identification of patients with IBP may offer a cost-effective way to reduce diagnostic delay by targetting investigations appropriately. <h3>Objectives</h3> We determined the prevalence of IBP in a UK primary care population using three published IBP criteria: Calin, Berlin and ASAS. <h3>Methods</h3> The study took place in a large general practice in Norfolk, UK, with 17,177 registered patients. Potential participants aged 18-80 years were identified from their primary care records as having consulted on at least one occasion with low back pain. A self-completed screening questionnaire was sent to a random sample of 978 patients asking about IBP symptoms. A further questionnaire was sent to non-responders. Patients were excluded from the study if they required nursing home care or had a terminal diagnosis. <h3>Results</h3> Five hundred and five completed questionnaires were returned (response rate 51.6%). Respondents had a median age of 60 years (IQR 48-67) and 44.8% were male. Eighty percent reported back pain of at least 3 months duration. The ASAS IBP criteria were fulfilled by 75 (14.9%) respondents, the Calin criteria by 132 (26.1%) and the Berlin criteria by 151 (29.9%). IBP was seen more commonly in women, but this difference was not statistically significant and there was no significant difference in prevalence between different age bands. The prevalence of IBP among patients with at least one previous consultation for back pain was 7.7% (95% CI 6.2, 9.5%) using the ASAS criteria, 13.5% (11.5, 15.8) using the Calin criteria and 15.4% (13.3, 17.8) using the Berlin criteria. <h3>Conclusions</h3> Estimations of the prevalence of IBP are significantly affected by the criteria chosen for classification. This should be considered when comparing studies, and when assessing patients in the clinical setting. <h3>Disclosure of Interest</h3> L. Hamilton Grant/research support from: Pfizer, Speakers bureau: MSD, A. Macgregor: None Declared, V. Warmington: None Declared, E. Pinch: None Declared, K. Gaffney Grant/research support from: Pfizer, Speakers bureau: Pfizer, UCB, Abbott
Improving climato-economic theorizing at the individual level
Authors: Ronald Fischer
Journal: Behavioral and Brain Sciences (2013)

Abstract Using representative data from 55 nations, I show that individual level wealth interacts with climate in predicting individual happiness but not postmaterialism values. I propose that more research is needed to identify (a) the specific mechanisms of how wealth buffers climatic demands at the individual level and (b) the neurocognitive and physiological reactions of individuals situated in different ecological niches.
Scintigraphy of Stress Fractures of the Sesamoid Bones
Authors: PANAGIOTIS GEORGOULIAS, IOANNIS GEORGIADIS, NIKOLAOS DIMAKOPOULOS, GEORGIOS MORTZOS
Journal: Clinical Nuclear Medicine (2001)

GEORGOULIAS, PANAGIOTIS M.D.; GEORGIADIS, IOANNIS M.D.; DIMAKOPOULOS, NIKOLAOS M.D.; MORTZOS, GEORGIOS M.D. Author Information
Cardiac septal umbrella for closure of a tracheoesophageal fistula
Authors: F. Coppola, G. Boccuzzi, G. Rossi, S. Gaia, M. Cosimato, S. Recchia
Journal: Endoscopy (2010)

We describe the use of a cardiac septal occluder (Amplatzer septal occluder [ASO]; AGA Corporation, Plymouth, Minnesota, USA), which is commonly adopted to close cardiac septal defects [1], but was used in the present case to treat an 83-year-old patient with a benign tracheoesophageal fistula (TEF). The TEF, which was caused by the accidental ingestion of dental amalgam, was previously treated unsuccessfully by the placement of a plastic covered stent, metal clips, and fibrin glue injection [2] [3] [4].