tbuckley/cabot-search huggingface dataset:
OP0145 Salivary Gland Ultrasonography Improves the Diagnostic Performance of ACR 2012 Classification Criteria for Sjögren’s Syndrome
Authors: D. Cornec, S. Jousse-Joulin, T. Marhadour, J.-O. Pers, Y. Renaudineau, A. Saraux, V. Devauchelle-Pensec
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Recently published ACR classification criteria for primary Sjögren’s syndrome (pSS) include only 3 objective tests: 1) a serological item: anti-SSA/SSB antibodies or [antinuclear antibody (ANA) titer ≥1:320 and rheumatoid factor (RF) positivity], 2) an Ocular Staining Score (OSS) ≥3, and 3) a focus score ≥1 on salivary gland biopsy (SGB). According to these criteria, a patient can be classified as pSS if almost 2 of these 3 items are present. None of these tests explore the function or the morphology of the salivary glands. In a recent study, we have shown that salivary gland ultrasonography (SGUS) has good diagnostic properties for pSS.<sup>1</sup> <h3>Objectives</h3> To evaluate if SGUS could improve the diagnostic properties of ACR 2012 criteria <h3>Methods</h3> The pSS Brittany cohort includes patients with suspected pSS (sicca symptoms, parotidomegaly or extraglandular manifestations suggestive of pSS). All patients had a standardized clinical and biological evaluation and a SGB. Ophtalmologic evaluation used fluorescein and lissamine green to assess the presence of keratoconjunctivitis sicca, allowing the subsequent calculation of the OSS. SGUS was performed on bilateral parotid and submandibular glands. Their echostructure was quoted on a scale between 0 and 4, as previously published.<sup>1</sup> A SGUS score ≥2 was considered pathologic. The gold standard for the analysis was a clinical diagnosis of pSS performed by a group of experts, unaware of the SGUS score. <h3>Results</h3> Eighty-eight patients were included in this study (mean age 57.0±13.0 years, symptoms duration 6.9±6.7 years, 94.3% females). The diagnosis of pSS was made in 40 patients, and 33 patients fulfilled ACR criteria. Sensitivity (Se) and specificity (Sp) of the different items for the diagnosis of pSS were respectively: 62.5% and 95.8% for the serological item of ACR criteria; 85.0% and 81.3% for the focus score ≥1; 60.0% and 60.0% for the OSS ≥3; 60.0% and 87.5% for SGUS. ACR criteria displayed 72.5% Se and 91.7% Sp. We created a new criteria set including the 3 items of ACR criteria and SGUS; these criteria were considered positive for all patients fulfilling almost 2 of these 4 items. The adjunction of SGUS to ACR criteria increased their Se to 90.0%, for a similar 89.6% Sp. <h3>Conclusions</h3> The diagnosis properties of ACR classification criteria for pSS are notably improved by the addition of the SGUS score. SGUS should be included in future consensual classification criteria for pSS. <h3>References</h3> Cornec D, Jousse-Joulin S, Pers J-O, Marhadour T, Cochener B, Boisramé-Gastrin S, et al. Contribution of salivary gland ultrasonography to the diagnosis of Sjögren’s syndrome: Toward new diagnostic criteria? <i>Arthritis Rheum</i> 2013;65:216-225. <h3>Disclosure of Interest</h3> None Declared
Senate Votes on NCI Appropriation
Authors: D. A. Tisevich
Journal: JNCI Journal of the National Cancer Institute (1991)

Journal Article Senate Votes on NCI Appropriation Get access Dorothy A. Tisevich Dorothy A. Tisevich Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 83, Issue 19, 2 October 1991, Pages 1378–1379, https://doi.org/10.1093/jnci/83.19.1378-a Published: 02 October 1991
AB0912 Effect of Corticosteroids on Bone Mineral Density and Body Composition in Patients with Juvenile Idiopathic Arthritis
Authors: R. Lahlou, S. Rostom, A. Hari, N. Bensaoud, R. Bahiri, N. Hajjaj-Hassouni
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Objectives</h3> Evaluate the association between corticosteroid therapy, body composition and bone mineral density in patients with juvenile idiopathic arthritis. <h3>Methods</h3> Our study was a cross-sectional study including Moroccan patients with juvenile idiopathic arthritis according to the International League of association of Rheumatology (ILAR) criteria, seen in rheumatology consultation or hospital. Sociodemographic and clinical data of the patients were collected. Bone mineral density of patients (BMD g/cm<sup>2</sup>) was measured by DXA X-ray Lunar Prodigy scanner. It was measured in two sites: the lumbar spine (L1, L4) and the whole body. Lean and fat mass were measured by the same device at the whole body and expressed in grams. <h3>Results</h3> 33 patients were included, the mean of their age was 10±4.35 years old, with a male predominance (54.5%), the median of disease evolution was 2 (1 - 4.5) years. Twenty for percent had a systemic JIA, 27% had an oligoarticular JIA and 48% had a polyarticular JIA. nineteen (57.6%) of our patients were receiving corticosteroid therapy, the median of daily intake dose was 10mg (0-15), the median of treatment duration was 3 years (1-6) and the median of cumulative dose was 10950mg (5475 -21 900). The median of BMD in the whole body, lumbar spine, lean mass and fat mass were respectively 0.720 g/cm<sup>2</sup> (0700-0900) 0.620 g/cm<sup>2</sup> (0530-0900), 20.49g (15.96-33.45), 4.97g (3.63-15.09). There was no significant correlation between the daily dose of corticosteroids and lean body mass (p=0.23), fat mass (p=0.21), BMD of the lumbar spine, p=0.82 or BMD of whole body p=0.86. We didn9t find a significant correlation between the cumulative dose of corticosteroids and lean body mass (p=0.079), fat mass (p=0.05), lumbar spine BMD (p=0.27) and the hole body BMD (p=0.36). <h3>Conclusions</h3> This study suggests that taking steroids does not affect the BMD or body composition in children with JIA. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5806
PTU-168 2-Stage oesophagectomy confers no survival advantage over transhiatal resection: analysis of 550 consecutive cases in a single unit
Authors: A Davies, H Sandhu, A Pillai, P Sinha, S Helme, J Deguara, J Gossage, A Botha, R Mason
Journal: Gut (2012)

<h3>Introduction</h3> The optimal operative approach to resectable cancers of the oesophagus and oesophago-gastric junction is contentious. Both transhiatal (THO) and 2-stage (2-ST) resections are routinely practised in our unit, largely according to individual surgeon preference. <h3>Methods</h3> A prospectively collected database containing 550 consecutive resections was available for analysis. All other variables (Investigation, MDT decision making and ITU input) were consistent within the unit. <h3>Results</h3> Between 2000 and 2010, 267 patients underwent THO and 283 had 2-ST oesophagectomy. Demographics showed equal characteristics between the groups with a median age of 65 years old and a predominantly male population. Adenocarcinomas made up 79% of resections. 330 (60%) patients underwent neo-adjuvant chemotherapy. 58% were pre-operatively staged as having stage 3 disease. In-hospital mortality was 1.1% (THO) vs 1.5% (2-ST). Hospital stay was similar between the two groups (median 14 days vs 15 days). Median survival on Kaplan–Meier analysis was 49 months for THO vs 34 months for 2-ST (p&lt;0.0005). Further analysis of the 2-ST procedures showed median survival of 40 months, 29 months and 23 months for laparoscopic assisted, left thoraco-abdominal and ivor-lewis resections respectively. <h3>Conclusion</h3> Only one randomised trial has ever compared the two operative approaches, demonstrating no survival advantage for more radical 2-Stage surgery. Quality of life data may support transhiatal resections. Our data suggests that transhiatal resections carry a low post-operative mortality with good long term survival rates that are at least comparable to 2-Stage procedures. Transhiatal oesophagectomy is a viable alternative in the treatment of oesophageal cancer. <h3>Competing interests</h3> None declared.
494 REAL-WORLD DATA ON TREATMENT MODIFICATION FROM A LONG-TERM OBSERVATIONAL STUDY OF CHRONIC HEPATITIS B PATIENTS FROM 5 EUROPEAN COUNTRIES
Authors: J.P. Zarski, V. Amara, H. Leblebicioglu, S. Zeuzem, B. Lescrauwaet, D. Kamar, I. Klauck, E. Morais, K. Simon
Journal: Journal of Hepatology (2012)
P592The effect of a high fat diet on cardiac and adipose mesenchymal cells.:
Authors: F Perbellini, DA Buchanan, RSM Gomes, S Vieira, K Clarke, G Faggian, C A Carr
Journal: Cardiovascular Research (2014)

Mesenchymal cells have been shown to improve contractile function in rodents following myocardial infarction. Here we determined the effect of simply a high fat diet on mesenchymal cells from cardiac and adipose tissues. Mice were fed a high-fat diet (55% fat; HFD,) for 4 months, after which cardiac explant-derived and cardiosphere-derived cells (EDCs, CDCs) were cultured from atrial tissue and the stromal vascular fraction (SVF) was isolated from epididymal fat depots and cultured to give adipose stromal cells (ASCs). Plasma metabolites were measured using an ABX Pentra. Cardiac protein expression was analysed by Western blotting and cells were characterised using qPCR, flow cytometry and immunocytochemistry. High fat-feeding raised fed plasma glucose, cholesterol and insulin levels and decreased plasma lactate, consistent with a type 2 diabetic phenotype. Significantly more ASCs were obtained from HFD animals and ASC numbers correlated with plasma glucose, lactate and cholesterol levels. There was an inverse correlation between the numbers of EDCs and the SVF but no significant differences between total numbers of EDCs or CDCs from chow-fed or HFD mice were found. The SVF and p2 ASCs from HFD mice contained significantly more CD45+ haematopoetic cells, than those from chow-fed mice, whilst p2 ASCs from HFD mice also contained more cells expressing the mesenchymal marker CD105 or the fibroblast marker DDR2. No differences in surface marker expression were observed between CDCs from HFD and chow-fed mice. Thus, whilst a high fat diet does not affect cardiac progenitor cells, it increases the number of fibroblasts and haematopoietic cells within the ASC population. Cell characterisation Surface marker expression in SVF, ASCs and CDCs from chow-fed and HFD mice, assessed using flow cytometry. * p < 0.05 vs cells from chow-fed mice. Cell characterisation Surface marker expression in SVF, ASCs and CDCs from chow-fed and HFD mice, assessed using flow cytometry. * p < 0.05 vs cells from chow-fed mice.
PWE-112 Prevalence of osteoporosis in a Liverpool coeliac cohort supports routine use of bone mineral density assessment
Authors: J Collum, A Smith, K Martin
Journal: Gut (2012)

<h3>Introduction</h3> Coeliac disease may be associated with osteoporosis and increased fracture risk. Osteoporosis is a significant public health problem with major consequences for patients and health care systems.<sup>1</sup> Debate exists concerning the utility of routine bone mineral density (BMD) assessment in patients with coeliac disease.<sup>2</sup> We aimed to identify the prevalence of osteoporosis in patients with coeliac disease as defined by BMD assessment. <h3>Methods</h3> Dietitian led data sets are currently maintained for all coeliac patients under active follow-up at University Hospital Aintree. We retrospectively analysed this information to determine (1) the frequency of BMD assessment in coeliac patients and, (2) the results of BMD assessment. Osteoporosis was defined as a T score of ≤−2.5 SDs below mean at either lumbar spine or hip. <h3>Results</h3> The data sets for 232 patients were available for analysis. Demographics: 70% female, 30% male, mean age at diagnosis 52 (range 3–79 years). BMD assessment was undertaken in 211 (91%). The indication for this assessment in all cases was a clinicopathological diagnosis of coeliac disease. Of those undergoing BMD assessment, 26% had osteoporosis. On questioning at outpatient assessment 141 (67%) patients reported participation in regular weight bearing exercise. Of these patients 33 (24%) had osteoporosis compared to 10/30 (33%) not documented to undertake weight bearing exercise (p=0.26). Of those undergoing BMD assessment, 128 (61%) had been prescribed calcium supplements. 49/128 (38%) patients documented to be taking calcium supplements had BMD measurements consistent with osteoporosis compared to 4/82 (5%) patients not taking calcium supplements. <h3>Conclusion</h3> At this UK centre, where over 90% of patients with coeliac disease underwent BMD assessment, 26% had osteoporosis. This is comparable to the rate demonstrated by Fitzgerald <i>et al</i> (25%)<sup>3</sup> and provides further support for the routine use of BMD assessment in coeliac disease to screen for osteoporosis. A lesser proportion of patients who participated in regular weight bearing exercise had osteoporosis at BMD assessment, but this finding was not statistically significant. <h3>Competing interests</h3> None declared. <h3>References</h3> 1. <b>Lewis NR</b>, Scott BB. <i>Guidelines for Osteoporosis in Inflammatory Bowel Disease and Coeliac Disease.</i> BSG Guidelines in Gastroenterology, 2007. http://www.bsg.org.uk/clinical-guidelines/ibd/guidelines-for-osteoporosis-in-inflammatory-bowel-disease-and-coeliac-disease.html 2. <b>Lewis NR</b>, Scott BB. Should patients with coeliac disease have their bone mineral density measured? <i>Eur J Gastroenterol Hepatol</i> 2005;<b>17</b>:1065–70. 3. <b>Fitzgerald T</b>, Davies H, Howdle PD, <i>et al.</i> Prevalence of osteoporosis in Leeds coeliac cohort. <i>Gut</i> 2011;<b>60</b>:A91.
MECHANISM OF LUMINAL WIDENING AND THE CORRECTION OF PLAQUE- AND CARINA- SHIFT AT SIDE BRANCH OSTIUM BY KISSING BALLOON INFLATION FOR CORONARY BIFURCATION LESIONS: VOLUMETRIC INTRAVASCULAR ULTRASOUND ANALYSIS
Authors: Masahiro Yamawaki, Murasato Yoshinobu, Toshiro Shinke, Kenichi Fujii, Hiromasa Otake, Shinichiro Yamada, Yoshihiro Takeda, Yoshihisa Kinoshita
Journal: Journal of the American College of Cardiology (2015)
Blockade of Notch Signaling by GSI Induces Apoptosis in Human Breast Cancer Cells through Various Molecular Mechanisms Involving Survivin and BH3-Only Members of the Bcl-2 Family.
Authors: C. Séveno, M. Campone, M. Campone, P. Juin, S. Barillé-Nion
Journal: Cancer Research (2009)

Abstract The Notch pathway is multifunctional and controls key nodes in cell signaling. In most cases, its deregulation has oncogenic effects, and aberrant activation of Notch signaling has been evidenced in breast cancer (Stylianou S, CR 2006). In particular, a functional Notch pathway appears to be of critical importance in the maintenance of basal breast cancer (Lee CW, CR 2008, BCR 2008). Moreover, because Notch signaling is repressed by both ER (Rizzo P, CR 2008) and HER2 (Osipo C, Oncogene 2008) oncogenic pathways, its reactivation during tamoxifen or trastuzumab treatment may restrain the efficiency of these inhibitors. Thus, Notch signaling inhibitors, such as competitive inhibitors of the g-secretase, GSI, that prevent proteolytic processing of the Notch receptor and the activation of its intracellular domain, are being actively investigated for the treatment of breast cancer, even though the mechanisms that might underlie their effects are still poorly understood.We first observed that GSI XII (z-Ile-Leu-CHO) was more effective in inducing apoptosis, as a single agent, on ER-/PR-/Her2- breast cancer cell lines than GSI X (z-Leu-Leu-Nle-CHO). This effect was associated with decreased expression of the anti-apoptotic proteins Survivin and XIAP and increased expression of, depending on cell lines, either NOXA or PUMA, two pro-apoptotic BH3-only members of the Bcl2 family.In the opposite, ER+ breast cancer cell lines were more sensitive to induction of apoptosis by GSI X, but this was manifest only in combination with Tamoxifen. Interestingly, Tamoxifen treatment of these cells induced both GSI X-sensitive Notch transcriptional activity and Survivin expression. Moreover, Survivin knock-down by RNA interference increased Tamoxifen sensitivity. Thus, Survivin might be an important actor of survival signal triggered by Notch (re)activation during ER pathway inhibition. Our results corroborate previous data showing the interest to use GSI in breast cancer but underline also the need to unravel the sub-type and context dependent molecular events involved in their biological activity. Citation Information: Cancer Res 2009;69(24 Suppl):Abstract nr 2160.
Abstract 1211: Role of anchorage-independence in signaling pathway activation and cell survival in invasive breast cancer cell lines
Authors: Julien Daubriac, Yves Boucher
Journal: Cancer Research (2010)

Abstract Resistance to anoikis - apoptosis induced by loss of matrix anchorage - is a key step in the metastatic process. In several cancer cell types including breast cancer cells, the anoikis-suppressing effect appears to be mediated by the PI3-kinase/Akt and MAP kinase signaling pathways. The aim of this study was to determine the involvement of signaling pathways in anoikis resistance in metastatic breast cancer cell lines. To study anoikis resistance, metastatic MDA-MB-231 and 4T1 cells were cultured on poly-HEMA-coated plates. Cell death was determined by detecting the sub-diploid population, by flow cytometry. Western analysis was used to assess the levels of phospho-Akt, phospho-p38 and cleaved-caspase-3. Our results show that loss of anchorage in MDA-231 cells reduces phospho-Akt levels, whereas in 4T1 cells - under both adherent and non-adherent conditions - the elevated level of phospho-Akt was not modified. Nevertheless, 4T1 cells undergo anoikis, which was dependent in part on the cleavage of caspase-3. In contrast, MDA-MB-231 cells were anoikis-resistant. Anoikis resistance correlated with the activation of p38 MAP-kinase. p38 phosphorylation was reduced in non-adherent 4T1 cells, whereas there was significantly higher levels of phosphorylated p38 in non-adherent than adherent MDA-MB-231 cells. The activation of p38 MAP kinase has been implicated in SDF1-CXCR4 signaling. One recent study has shown that CXCR4 inhibits anoikis in MDA-MB-231 cells, suggesting that CXCR4 or p38 blockade may induce anoikis in breast cancer. Thus our findings show that two metastatic breast cancer cell lines exhibit different sensitivity to anoikis, which is potentially associated with p38 MAP-kinase activity. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1211.
CRITERIA FOR THE SELECTION OF A SMALL GROUP OF CHRONIC SCHIZOPHRENIC SUBJECTS FOR BIOLOGICAL STUDIES:
Authors: SEYMOUR PERLIN, A. RUSSELL LEE
Journal: American Journal of Psychiatry (1959)

1. The method of selection of a small group of schizophrenic subjects for long-term biological studies is an essential part of the research. In developing criteria the following concepts are considered: (a) range of variation of irrelevant variables, (b) random sampling, nonpurposive bias and purposive bias, (c) extension of the sample and intensification of pertinent characteristics of patients through the process of selection and evaluation. 2. Variables presumbly irrelevant to schizophrenia are restricted: e.g., age, sex, race, length of hospitalization and complications of hospitalization and treatment. The acceptance of limitations inherent in a chronic schizophrenic sample presupposes that schizophrenia is a reflection of an on-going psychophysiological process (which however may appear only under certain operant conditions). The need for the study of acute schizophrenic and other samples is stressed. 3. An operational framework for biological versus psychological factors in schizophrenia is considered; the assumptions involved are tabulated. The working hypothesis assumes that there is a group of schizophrenias. Within this framework, process schizophrenics, catatonic and hebephrenic subtypes and twins concordant or discordant as to schizophrenia are discussed as means for sample bias. 4. A genetic-familial design is presented as the basis for a biased sample. It was decided that the widest sampling of genetic causes would be achieved if one-fourth of the sample had a family history suggesting dominance; one-fourth a history suggesting recessiveness, and one-half a negative family history. The patients with negative family history would be more likely to include an uncommon genetic type and also would render less likely the exclusion of the very group for which selection-bias is intended. The minimal conditions are as follows: (a) Family History Group–The Dominant Pattern; one schizophrenic parent; (b) Family History Group–The Recessive Pattern; neither parent affected but with at least one affected sibling; (c) Negative Family History Group; no history of schizophrenia within the family unit. 5. The decreasing number of subjects available from a patient population of 13,500 in 3 collaborating hospitals is noted as the following additional criteria are imposed: diagnosis of schizophrenia, male, white, age range 18-40, duration of hospitalization of more than one year and less than 15 years, and familial pattern. A comparison of Family History and Negative Family History Groups with the initial criteria for selection is made; data referring to the first 14 admissions are presented.
Abstract 2552: Detection of PIGO-deficient cells using proaerolysin: a valuable tool to investigate mechanisms of mutagenesis in the DT40 cell system
Authors: Jun Nakamura, Xu Tian, Scott Bultman, James A. Swenberg
Journal: Cancer Research (2012)

Abstract A DNA damage response analysis makes use of the chicken DT40 isogenic cell line and its dozens of available mutants knocked out in various DNA repair and cell cycle pathways. While this DT40 cell system is valuable for understanding the DNA damage response to genotoxic agents, no convenient mutation assay for mutagens currently exists for this reverse-genetic system. Here we establish a proaerolysin (PA) selection-based mutation assay in DT40 cells to identify glycosylphosphatidylinositol (GPI)-anchor deficient cells. Using PA, we detected an increase in the number of PA-resistant DT40 cells exposed to MMS for 24 hours followed by a 5-day period of phenotype expression. GPI anchor synthesis is catalyzed by a series of phosphatidylinositol glycan complementation groups (PIGs). The PIG-O gene is on the sex chromosome (Chromosome Z) in chicken cells and is critical for GPI anchor synthesis at the intermediate step. We identified that all PA-resistant DT40 cells analyzed have mutations in the PIG-O gene. This novel assay for DT40 cells provides a valuable tool to investigate the mode of action of mutations caused by reactive agents using a series of isogenic mutant DT40 cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2552. doi:1538-7445.AM2012-2552