tbuckley/cabot-search huggingface dataset:
Abstract 3025: CXCR4 is expressed in the nucleus of metastatic prostate cancer cells
Authors: Ayesha S. Don Salu Hewage, Cimona V. Hinton
Journal: Cancer Research (2012)

Abstract Most prostate cancer-related deaths are due to the advancement of the tumor to a metastatic disease. The C-X-C Chemokine Receptor 4 (CXCR4) is a G-protein coupled receptor (GPCR) involved in the metastasis of various cancers, including the prostate. CXCR4 is generally regarded as a plasma membrane receptor; the canon concept of GPCR signaling holds that receptors located at the plasma membrane respond to secreted agonist stimulation. GPCRs, however, have also been observed at other intracellular organelles, including the nuclear membrane. Several cancer models have positively observed expression of a nuclear form of CXCR4 in advanced stage tissues and tumors with a poor prognosis. The significance of these receptors having a dualistic localization pattern is that nuclear receptors can escape from chemotherapy, since most therapeutics are hydrophobic small molecules targeted towards membrane bound receptors, which cannot pass through non-polar regions of the plasma membrane. An initial search of the CXCR4 sequence through the PredictNLS software program revealed a putative, nontraditional nuclear localization sequence, RPRK. We probed prostate cancer tissues for CXCR4 and observed a higher nuclear expression in high grade prostate tumors, compared to low grade, and no detectable CXCR4 in normal human prostate tissue by immunohistochemistry. Subcellular fractionations of prostate cancer cell lines (PC3, DU145 and 22RV1) and normal prostate epithelial cells (RWPE1) demonstrated positive expression of CXCR4 in both nuclear and cytoplasmic fractions, which were confirmed by immunocytochemistry analysis, independent of exogenous SDF1α stimulation. Based on our results, we suggest that CXCR4 is alternately expressed in the nucleus of advanced prostate cancer, which may enhance prostate cancer metastasis. To determine the origin of nuclear CXCR4, the a kinetic localization study confirmed that CXCR4 translocates to the nucleus from the plasma membrane at 37°C, which was inhibited when the same reaction was performed at 4°C. In conclusion, expression and localization of a nuclear CXCR4 may contribute to the advancement of prostate cancer to a metastatic disease, and aid tumors in escaping from therapy. Our studies will contribute to the development of therapeutics towards CXCR4, as current options are targeted towards membrane receptors, which may be ineffective if CXCR4 located and functional inside of the cell. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3025. doi:1538-7445.AM2012-3025
Post‐treatment supportive care for the natural dentition and dental implants
Authors: Gary C. Armitage, Pinelopi Xenoudi
Journal: Periodontology 2000 (2016)

Abstract Long‐term successful treatment of chronic periodontitis requires placement of patients on post‐treatment recall programs known as either periodontal maintenance therapy or supportive periodontal therapy. Selection of the recall intervals must be based on the specific needs of individual patients. A single recall interval (e.g. 6 months) is not suitable for all patients. The main purpose of these programs is to prevent the recurrence of periodontitis. The components of every periodontal maintenance therapy program include: review of medical/dental histories; complete oral examination with an emphasis on the detection of gingival inflammation; establishing whether the maintenance program is working by monitoring clinical attachment levels; evaluation of oral hygiene; and full‐mouth supragingival and subgingival debridement (i.e. biofilm removal). Long‐term post‐insertion care for dental implants also requires a similar patient‐specific recall program of supportive implant therapy. The main purposes of a supportive implant therapy program are to maintain a healthy peri‐implant mucosa and thereby prevent the development of peri‐implantitis. In cases in which plaque‐induced peri‐implant mucositis has occurred, a well‐designed supportive implant therapy program can help return the mucosa to a healthy state. At the current time there is no consensus on the optimal interventions for the treatment of peri‐implant mucositis. However, all effective supportive implant therapy programs emphasize meticulous oral hygiene practices, careful peri‐implant examination, thoughtful analysis of risk factors and periodic removal of microbial deposits from the implants.
“Flush sign” is a possible predictor of adhesions in EUS-guided transmural drainage of pancreatic fluid collections
Authors: S Itaba, A Aso, H Kubo, H Igarashi, H Akiho, K Nakamura, T Ito, R Takayanagi
Journal: Endoscopy (2011)

Background and aims: Endoscopic ultrasound (EUS)-guided transmural drainage is an alternative to surgical or percutaneous drainage of inflammatory pancreatic fluid collections (PFCs). It is important, but sometimes difficult, to predict adhesions between PFCs and the digestive tract wall in EUS-guided transmural drainage. The aims of this study were to compare perforation and non-perforation cases in EUS-guided transmural drainage, and to establish methods for predicting such adhesions.
Abstract 2407: Decreased inflammation and tumor stage in COX-2 knockout mice in the BBN model of bladder cancer
Authors: Shilpa Choudhary, Poornima Hegde, Carol Pilbeam, John A. Taylor
Journal: Cancer Research (2011)

Abstract Introduction and Objective: Prostaglandins (PG) play a critical role in the development of solid tumors. Inhibition of cyclooxygenase-2 (COX-2), the committed step in PG synthesis, has been shown to impair tumor development in the BBN mouse model of bladder cancer. Because inhibitors of COX-2 have been reported to have anti-tumorigenic properties independent of COX-2, we evaluated the impact of gene disruption of COX-2 in the BBN mouse model. Methods: We used 2 mo old male COX-2 knockout (KO) and wild type (WT) mice in an outbred CD-1 background. Mice were treated with (n=13-14 / group) and without (n=6/group) BBN in drinking water and euthanized after 25-27 wks. Bladders were harvested, weighed, bisected and step sectioned after formalin fixation. H&amp;E stained sections were evaluated for pathologic stage/grade, percent involvement by invasive disease and degree of inflammatory infiltrate(grade 1-3). Results: All animals receiving BBN had inflammatory changes at the time of euthanasia, with the grade being higher in WT (grade 2-3) than KO mice (grade 1-2). Six WT mice and 3 KO mice receiving BBN died, all within the first 18 wks of study, without gross evidence of tumor. Compared to previous studies with C57Bl/6 mice, tumor development was slower and burden was lower at 25-27 wks. All 7 BBN-treated WT mice had high grade invasive cancer at euthanasia: 6 pT1 and 1 pT3. Out of 11 BBN-treated KO mice at euthanasia, 10 had high grade cancer and 2 had invasive disease: 1 pT0, 6 CIS, 2 pTa (non-invasive) and 2 pT1. Mean bladder weight at euthanasia was elevated only in BBN-treated WT mice. There was a strong correlation between WT genotype and tumor stage by Pearson's R (0.74, p=0.03). The percent of tumor representing invasive disease was 0.31 in COX-2 WT mice vs 0.01 in KO mice (t-test, p=0.002). Conclusions: Genetic disruption of COX-2 resulted in lower tumor stage and percent invasiveness in the BBN mouse model of bladder cancer. However, most COX-2 KO mice developed high grade disease, indicating disruption of COX-2 was not sufficient to prevent tumorigenesis. Reduced inflammation in the COX-2 KO mice may account for the decreased invasiveness. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2407. doi:10.1158/1538-7445.AM2011-2407
A2.14 Human mast cells engulf and store exogenous IL-17A
Authors: T Noordenbos, J Paramarta, I Blijdorp, L van Mens, J Stap, R Hoebe, E Mul, E Lubberts, N Yeremenko, DL Baeten
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> The emerging role of IL-17A in immune-mediated inflammatory diseases urges characterisation of the cellular source. Numerous recent studies have described the presence of IL-17A-positive mast cells in inflamed target tissues. Since mouse studies have not indicated mast cells as IL-17A producers, we investigated the mechanism of IL-17A expression by human mast cells. <h3>Methods</h3> Quantitative PCR and Western blot analysis was performed on mast cells and CD4<sup>+</sup> T-cells FACS-purified from human tonsils, <i>ex vivo</i>and after stimulation with PMA/ionomycin. Uptake of exogenous protein was analysed by microscopy, imaging-flowcytometry, live imaging and Western blot. DynaminII-inhibitor was used to block endocytosis. <h3>Results</h3> Strikingly, gene expression analysis of highly purified primary mast cells failed to detect both IL-17A, IL-17F and their transcription factor RAR-related orphan receptor C (RORC), even after stimulation. Although RORC was also absent on the protein level, IL-17A was abundantly detected, conflicting with the mRNA data. Subsequent analysis of subcellular location showed the presence of IL-17A in lysosomal storage granules and in early endosomes, suggesting a role for endocytosis. We investigated whether mast cells are capable of internalising exogenous IL-17A <i>in vitro</i>. Microscopy, imaging-flowcytometry and Western blot analysis indicated that LAD2 mast cell line as well as primary mast cells are able to engulf exogenous IL-17A, but not TNFa. Live imaging revealed further that internalisation of IL-17A is specific and can be halted by blocking clathrin-mediated endocytosis. <h3>Conclusion</h3> This data strongly suggest that human mast cells do not produce IL-17A, but rather engulf exogenous IL-17A from the inflamed milieu and store it in granules. Molecular mechanisms of IL-17A uptake and the contribution of this process to disease are currently under investigation.
M147 Feasibility Of Delivering An Occupational Health Intervention Aimed At Improving Work Productivity, Among Working Copd Patients
Authors: K. Kalirai, P. Adab, R. Jordan, J. Ayres, S. Sadhra
Journal: Thorax (2014)

<h3>Introduction</h3> There is evidence that workplace productivity may be impaired among working patients with COPD. Occupational health (OH) interventions have been effective in improving work productivity in other chronic conditions. However, little is known about the feasibility and acceptability of such interventions among those with COPD. <h3>Aim</h3> To assess feasibility and acceptability of an OH intervention in working COPD patients. <h3>Methods</h3> Nested within a primary care COPD cohort (n = 1870), the study included all those who were in work (n = 309). Eligible patients were invited for an interview and assessment with an OH practitioner. The aim was to explore and identify workplace factors that may contribute to their work performance or exacerbate their condition, and to suggest approaches to minimise any respiratory symptoms and improve work capability. Recommendations are sent to the patient, and with their permission, to their GP and employer. The acceptability of the intervention to employers will be explored as a separate part of the study. <h3>Results</h3> Of those eligible, 43 (13.9%) agreed to take part and 107 (34.6%) declined. The most common reasons for declining included a perception of being fine at work (49.5%); followed by believing that work adjustments had already been made (7.5%); and worry about involving the employer (4.7%). Patients agreeing to take part were more likely to be male (71.4% vs. 56.2%), ever-smokers (100% vs. 89.5%), slightly older (mean age 61.9 vs. 60.9) and have more severe airflow obstruction (19.5% vs. 12.5%) than those declining. One or more OH recommendations were identified for 14/16 (87.5%) patients seen so far. These included: avoid substances/materials at work (56.3%); modify physical aspects of job (43.8%); modify job tasks/work methods (37.5%); modify work environment (18.8%), change work organisation (31.3%); use respiratory protective equipment (6.3%). <h3>Conclusions</h3> This is the first study to assess the feasibility of delivering an OH intervention to patients with COPD working in diverse occupations. Although uptake rates were low, modifiable factors in the work environment that could improve their symptoms and condition were identified for the majority who were assessed. The acceptability of recommendations and feasibility of involving the employer will be further explored.
Abstract 5104: Cell-surface receptor expression in osteosarcoma
Authors: David S. Geller, Sheref E. Hassan, Richard Gorlick
Journal: Cancer Research (2010)

Abstract Purpose: Although numerous cell-signaling receptors have been described in the setting of osteosarcoma, the comprehensive characterization of receptors on the cell-surface has not been described. The goal of the current study was to characterize and quantify the expression of cell-surface receptors across multiple standard osteosarcoma cell lines and patient samples placed in culture. Methods: Both standard (n=4) and patient derived (n=10) osteosarcoma cell lines were cultured and labelled with a panel of commercially available flurophore-bound antibodies including antibodies to EGFR, HER-2, HER-3, HER-4, IGF-1R, IGF-2R, IR, VEGFR-1, VEGFR-2, VEGFR-3, CMET, FGFR-2, FGFR-3, PDGF alpha, and PDGF beta. Standard cell lines utilized were 143b, HOS, Saos, and U205. Patient samples included samples 229, 231, 232, 238, 252, 293, 308, 311, 242, and 290. Background fluorescence was accounted for using either a known negative control cell line if available or the antibody-specific IgG isotype. Unlabelled cells were also evaluated for inherent fluorescence. Cell analysis was performed using flow cytometry (FACS) and data was analyzed using FloJo software. The geometric fluorescent means were calculated and compared against positive controls. Results: Although a degree of variability in cell-surface receptor expression existed between the different cell lines, preliminary results show high expression of PDGFR-beta in all 14 cell lines, with an average geometric mean of 1376 (range 93.8 - 4496). A higher expression of IGF-1R was also noted across all cell lines, with an average geometric mean of 881 (range 122 - 3191). In depth analysis of receptor expression and the interrelationship of these various receptors is ongoing. Conclusion: Preliminary results demonstrate PDGFR-Beta and to a lesser extent IGF-1R to be consistently expressed on the cell-surface in all tested osteosarcomas cell lines. Data analysis of receptor expression and interrelationships between receptor expression is ongoing. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5104.
Abstract 3200: Spliced variant of T-cell intercellular antigen 1 (TIA-1) in colorectal cancer.
Authors: Maryam Hamdollah-Zadeh, Xi Ye, Enric Domingo, David J. Kerr, Mike R. Ladomery, Steve J. Harpar, Dave O. Bates
Journal: Cancer Research (2013)

Abstract Alternative splicing regulates many cancer-associated genes. Splice isoforms that are found in tumors could have diagnostic value and may provide potential drug targets. We identified an endogenous splice variant of TIA-1 (T-cell intercellular antigen-1), short TIA-1 (shTIA-1), expressed in invasive colon cancer cells, which was not expressed in non-invasive adenoma cell lines. In this study we investigated the frequency of shTIA-1 expression in human normal and cancer tissue and if it correlates with advanced-stage of colon cancer. RNA was extracted from 25μm thick scrolls of formalin fixed paraffin embedded sections of 40 colorectal carcinoma (20 Duke's, 20 Duke's C stage) and normal samples. RT-PCR was carried out to detect full length (flTIA-1) and shTIA-1. Immunohistochemical CD31 staining of microvessels in the same samples was used to assess microvessel density (MVD) and correlate the shTIA isoform expression and stages of the tumors. The results showed that flTIA-1 was less frequently found in tumour than in normal tissues (p=0.002). shTIA-1 was more highly expressed than flTIA-1 and in significantly higher portion of tumors than normal samples (p=0.0106). There was a significantly lower proportion of Duke's B than Duke's C grade tumours with detectable shTIA-1 expression (p=0.018). CD31 staining showed samples with shTIA-1 associated with increased MVD. We did not find any association between stages and MVD, as there was no statistically significant difference in MVD between Duke's B and Duke's C. However tumours had higher MVD than normal tissues from the same patients. These results indicate that a splice variant of TIA-1 is expressed in human colorectal carcinoma and is associated with increased angiogenesis. Citation Format: Maryam Hamdollah-Zadeh, Xi Ye, Enric Domingo, David J. Kerr, Mike R. Ladomery, Steve J. Harpar, Dave O. Bates. Spliced variant of T-cell intercellular antigen 1 (TIA-1) in colorectal cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3200. doi:10.1158/1538-7445.AM2013-3200
SP0046 Improving your Graphs for Publications
Authors: M. Boers
Journal: Annals of the Rheumatic Diseases (2013)

<h3></h3> This workshop is an introduction to the principles of good graph design as pioneered by Cleveland<sup>1</sup> and Tufte<sup>2</sup> and updated by Few<sup>3</sup> so that the participant can better answer the following questions: <i>Which of the messages in my research results requires a graph?</i> Recognizing how graphs improve on simple statistics and convey much more information. <i>How can I best convey the message?</i> Striving for clear vision by choice of graph, scaling, discrimination of data series, minimizing non-data ink, avoiding chart junk. Striving for clear understanding through a balance between data and explanation. <i>Is my graph truthful?</i> Creating a direct proportion between graph and data quantities, avoiding forms prone to misinterpretation, labels to prevent ambiguity; keeping data in context, avoiding more dimensions in the graph than in the data. Cleveland WS. The elements of graphing data: Hobart Press, Summit, NJ, U.S.A.; 1994. Tufte E. The visual display of quantitative information. 2nd ed: Graphics Press, Cheshire, CT, U.S.A.; 2001. Few S. Show me the numbers. Designing tables and graphs to enlighten. Analytics Press, Oakland, CA, U.S.A.; 2004. <h3>Disclosure of Interest</h3> None Declared
THU0254 Treatment for Metatharsalgia by Plantar Insoles and Silicone Orthosis for Toes in Patients with Rheumatoid Arthritis and Osteoarthritis: A Cross-Over Study
Authors: S. Maddali Bongi, A. Del Rosso, E. Cavigli, B. Ferretti, M. Matucci Cerinic
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> In Rheumatoid Arthritis (RA) and Osteoarthritis (OA) forefoot involvement causes disability and metatharsalgia. <h3>Objectives</h3> to evaluate, in RA and OA patients, the efficacy of two programs combining insoles in polypropylene terephtalate (PPT) and custom silicone orthosis for toes. <h3>Methods</h3> 24 women (13 with OA e 11 with RA) with metatharsalgia were assigned at T0 to 2 groups (each formed by 12 patients): group A was treated for 30 days with PPT insoles (T1) and in the further 30 days silicone orthosis for toes were added (T2). Group B was treated for 30 days with PPT insoles and silicone orthosis (T1) and for the next 30 days only with insoles (T2). At T0, T1 and T2, pain, disability and functional limitation (by Foot Function Index -FFI-); plantar pressure (KPA) and areas (cm2) (by baropodometer) were assessed. At T0 and T2 a questionnaire evaluating global pain (5 VAS scales 0-10) and at T2 a VAS scale (0-10) assessing satisfaction were administered. <h3>Results</h3> In Group A, insoles application improves at T1 <i>versus</i> T0 all FFI sub-scales that, at T2 (after insoles and orthosis) result stable <i>versus</i> T1, and better <i>versus</i> T0 (p&lt;0.05). At T1 <i>versus</i> T0, plantar pressures improve (p&lt;0.05), with the results maintained at T2 <i>versus</i> T0 (p&lt;0.05) and no difference between T2 and T1 (p=NS). Plantar areas improve at T2 versus T0 (p&lt;0.05). In group B, at T1, the treatment with insoles and digital orthosis improves all FFI sub-scales <i>versus</i> T0. (p&lt;0.05). FFI-pain and FFI–disability remain better at T2 than at T0 (p&lt;0.05). At T2 <i>versus</i> T1, with the use of only insoles, FFI-pain impairs (p&lt;0.05). Plantar pressures at T1 improve in respect to T0 (p&lt;0.05), do not change at T2 <i>versus</i> T1, with the improvement maintained at T2 <i>versus</i> T0 (p&lt;0.05). Plantar areas do no change at any time point (p=NS). At T2, satisfaction from the treatment results 6.65 ± 1,56 in group A and 5.90 ± 2,16 in Group B (p: NS) and global pain improves <i>versus</i> T0 in both groups (p&lt;0.05). <h3>Conclusions</h3> In patients with RA and OA with metatharsalgia, silicone toe orthosis and PPT insoles improve foot pain, disability and pressures, especially with the protocol using firstly insoles and then adding toe orthosis. <h3>Disclosure of Interest</h3> None Declared
Leukemic Infiltration Mimicking Epididymo-Orchitis on Scrotal Scintigraphy
Authors: H H Mehta, S Thirumala, C J Palestro
Journal: Clinical Nuclear Medicine (1997)

With a sensitivity well in excess of 90%, scrotal scintigraphy can reliably differentiate testicular torsion from other conditions, notably epididymo-orchitis, that present as acute scrotal pain. In contrast to the photopenia associated with testicular torsion, inflammatory processes usually present with hyperperfusion and hyperemia of the affected site; in epididymo-orchitis the inflammatory changes involve the entire ipsilateral side, whereas in epididymitis, these changes are typically curvilinear and confined to the lateral aspect of the scrotum. The authors encountered a case of leukemic infiltration of the epididymis that mimicked epididymoorchitis on scrotal scintigraphy.