tbuckley/cabot-search huggingface dataset:
Heparin and Bone Marrow–Derived Cell Therapy
Authors: Edward T.H. Yeh, James T. Willerson
Journal: Circulation Research (2012)

2][3][4][5][6][7] In harvesting, storing, or injecting the bone marrow-derived mononuclear cells (BMCs), an anticoagulant, such as heparin, is often added to prevent cell clumping.In this issue of Circulation Research, Drs Stephanie Dimmeler, Andreas Zeiher, and their colleagues (Seeger et al 8 ) show in detailed studies that heparin may impair the functional capacities of BMCs.This discovery has significant implications for the processing of BMCs before administration of cell therapy and potentially in the treatment of patients with acute coronary syndromes, chronic ischemic and nonischemic cardiomyopathies, and peripheral vascular disease.
Cytokeratin expression in trichoblastic fibroma (small nodular type trichoblastoma), trichoepithelioma and basal cell carcinoma
Authors: None YAMAMOTO, None ASAHI
Journal: British Journal of Dermatology (1999)

Classical trichoblastic fibroma or small nodular type trichoblastoma (Ackerman) is a rare tumour. This tumour, trichoepithelioma and basal cell carcinoma (BCC) have some overlapping histopathological features. There are only a few reports on immunohistochemical studies in large series of these three neoplasms. We investigated immunostaining patterns of 10 different anticytokeratin (CK) antibodies and several other markers in these neoplasms, comparing them with the patterns in normal adult and fetal skin. In trichoblastic fibroma (three cases), CK1/5/10/14, CK7, CK8/18, CK10/11, CK14, CK17 and CK19 were expressed in the basaloid nests, and CK6 and involucrin were detected in the inner layers of keratinous cysts. Trichoepithelioma (seven cases) expressed CK1/5/10/14, CK8/18, CK14, CK17 and CK19 in the basaloid nests, and CK6, CK10, CK10/11 and involucrin were positive in the keratinous cysts. However, no CK7 expression was observed. Solid and keratotic types of BCC (29 cases) expressed CK1/5/10/14, CK7, CK8/18, CK14, CK17 and CK19 in the basaloid nests. The keratinous cysts in BCC were stained with anti-CK6, CK10, CK10/11 and involucrin antibodies. Coupled with the expression of CK8/18, CK17 and CK19 in the outer root sheath of the adult hair follicle, these three neoplasms shared a keratin phenotype characteristic of the outer root sheath. Judging from our immunohistochemical results, trichoblastic fibroma and BCC cannot be differentiated by their patterns of CK expression. The expression of CK7, which is noted in fetal hair follicles, trichoblastic fibroma and BCC, suggests the presence of subpopulations that retain fetal phenotypic characteristics in these two neoplasms. Although the current concept regards trichoepithelioma and trichoblastic fibroma as a single tumour group, the lack of CK7 expression in trichoepithelioma supports the notion that the two are different.
Serum IgG antibodies to peptidylarginine deiminase 4 in rheumatoid arthritis and associations with disease severity
Authors: E H Halvorsen, S Pollmann, I-M Gilboe, D van der Heijde, R Landewe, S Odegard, T K Kvien, O Molberg
Journal: Annals of the Rheumatic Diseases (2007)

<h3>Background:</h3> Antibodies targeting citrullinated antigens are specific for rheumatoid arthritis (RA). Citrullination is catalysed by the peptidylarginine deiminase (PAD) enzyme family. Critical enzymes are often targeted by disease-specific antibodies in complex immune-mediated diseases. Here, we have tested for autoantibodies against human recombinant PAD4 (hPAD4) in Caucasian RA patients. <h3>Methods:</h3> A time-resolved fluorometric immunoassay based on hPAD4 was developed to analyse sera from two RA cohorts (n = 237 and n = 177), one systemic lupus erythaematosus (SLE) cohort (n = 84) and 148 healthy controls. Simple and multiple analyses were performed to examine possible associations between anti-hPAD4 and disease variables. <h3>Results:</h3> Raised levels of anti-hPAD4 IgG were found in both RA cohorts compared to the controls, and 23% of the RA patients were anti-hPAD4 IgG positive. Anti-hPAD4 was associated with anti-cyclic citrullinated peptide (CCP) and rheumatoid factor (RF), as well as increased physical disability. Anti-hPAD4 was also associated with higher longitudinal radiographic damage scores and increased clinical joint pathology, but weaker than anti-CCP. No associations were found between anti-hPAD4 and selected Human leukocyte antigen (HLA)-DRB1 variants. <h3>Conclusions:</h3> Approximately 23% of Caucasian RA patients have serum IgG antibodies against hPAD4.The presence of serum anti-hPAD4 IgG was in simple analyses associated with a more severe disease phenotype, and the association with physical disability was maintained in multiple analyses.
Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
Authors: G Koopman, K H Heider, E Horst, G R Adolf, F van den Berg, H Ponta, P Herrlich, S T Pals
Journal: The Journal of Experimental Medicine (1993)

A recently described splice variant of CD44 expressed in metastasizing cell lines of rat tumors, has been shown to confer metastatic potential to nonmetastasizing rat pancreatic carcinoma and sarcoma cell lines. Using antibodies raised against a bacterial fusion protein encoded by variant CD44 sequences, we have explored the expression of variant CD44 glycoproteins on human lymphoid cells and tissues and on non-Hodgkin's lymphomas. Normal lymphohematopoietic cells express barely detectable low levels of variant CD44 glycoproteins, whereas T lymphocytes, upon activation by mitogen or antigen, transiently upregulate expression of specific CD44 variant glycoproteins. The reaction pattern of various antibodies indicates that these CD44 variants contain the domain encoded by exon v6, which is part of the variant that in the rat confers metastatic capability. It is interesting that overexpression of v6 was also found in several aggressive, but not low-grade, non-Hodgkin's lymphomas.
Cigarette Smoking and Other risk Factors for progression of Precancerous Stomach Lesions
Authors: R. W. Kneller, W.-C. You, Y.-S. Chang, W.-D. Liu, L. Zhang, L. Zhao, G.-W. Xu, J. F. Fraumeni, W. J. Blot
Journal: JNCI Journal of the National Cancer Institute (1992)

Background: Stomach cancer is generally thought to evolve through a series of gastric mucosal changes, but the derterminants of the precancerous lesions are not well understood. purpose : Our purpose was to assess risk factors for intestinal metplasia and gastric dysplasia arising from chronicatrophic gastritis in a genral population at high risk for stomach cancer. Methods : A population-based gastroscopic screening of more than 3000 residents was conducted in a county in china eith one of the world's highest rtes of stomach cancer. Information on the lifestyle and other charateristics of the participants was obtained by interview, and responses were compared between those in whom the most advanced gastric lesion was dysplasiaor intestinal metaplasia versus those with chronic atrophic gastritis. Results : Cigarette smoking was found to nearly double the risk of transition to dysplasia and to be a mild risk factor for intestinal metaplasia. Smoling accounted almost entirely for the 55% higher prevalence of dysplasis among men than among women. Risk of transition to dysplasis had a weak association with several dietary factors and was increased among those participants with a family history of stomach cancer and with blood type A. Conclusions: The findings provide storng evidence for a role of tobacco consumption and offer clues to other enviromental and genetic factord involved in the process of gastric carcinogenesis. (J Natl cancer Inst 84: 1261–1266, 1992]
Risk of Second Cancer in Nongastric Marginal Zone B-Cell Lymphomas of Mucosa-Associated Lymphoid Tissue: A Population-Based Study from Northern Italy
Authors: Luca Arcaini, Sara Burcheri, Andrea Rossi, Cristiana Pascutto, Francesco Passamonti, Ercole Brusamolino, Marco Paulli, Ester Orlandi, Maurizio Buelli, Piera Viero, Marco Lucioni, Francesca Montanari, Michele Merli, Sergio Cortelazzo, Mario Lazzarino
Journal: Clinical Cancer Research (2007)

The aim of this study was to define the risk of second cancer in nongastric marginal zone lymphomas of mucosa-associated lymphoid tissue (MALT).We considered for the analysis 157 patients with a confirmed histology of marginal zone B-cell lymphoma of MALT, presenting with a clinically prevalent extranodal site of disease, except for stomach. All patients came from two hematologic institutions of Northern Italy. We compared the occurrence of second cancer with respect to the general population by calculating the standardized incidence ratio, with the age- and sex-specific incidence rates of a cancer registry of Northern Italy (Lombardia) as a reference.A history of solid neoplasia was present in 29 (18%) patients for a total number of 30 neoplasms: 25 solid tumors, 2 hematologic diseases (1 Hodgkin's lymphoma and 1 essential thrombocythemia), and 3 nonmelanoma in situ skin cancers. In 4 patients, the site of cancer and lymphoma was the same. In 21 cases the solid tumor preceded the MALToma, in 3 the neoplasm was concomitant, whereas in 6 it was subsequent. For the entire group, the standardized incidence ratio of an additional malignancy was 0.8 [95% confidence interval (95% CI), 0.55-1.17; P = 0.2]. After excluding nonmelanoma skin cancer, the standardized incidence ratio of a second tumor was 0.75 (95% CI, 0.5-1.12; P = 0.2). After excluding all previous malignancies, the standardized incidence ratio of a second cancer was 1.32 (95% CI, 0.69-2.55; P = 0.4). The comparison of risks between males and females was not significant in each group analysis.Patients with nongastric MALT lymphomas are not at increased risk for other neoplasms compared with the general population of the same geographic area.
Heterogeneous changes in epimyocardial microvascular size during graded coronary stenosis. Evidence of the microvascular site for autoregulation.
Authors: H Kanatsuka, K G Lamping, C L Eastham, M L Marcus
Journal: Circulation Research (1990)

The purpose of this study was to determine the coronary microvascular sites of autoregulation. The epimyocardial coronary microcirculation was observed through an intravital microscope by stroboscopic epi-illumination in anesthetized open-chest dogs (n = 20). Aortic pressure and heart rate were held constant by an aortic snare and atrial pacing, respectively. Distal pressure of the left anterior descending coronary artery was controlled by a screw occluder on the proximal left anterior descending coronary artery and monitored with a 24-gauge plastic cannula inserted into the branch or distal portion of the left anterior descending coronary artery. Distal pressure of the left anterior descending coronary artery was stepwisely reduced to 59 +/- 1 mm Hg (mild stenosis, n = 20) and 38 +/- 1 mm Hg (severe stenosis, n = 16). In the left circumflex coronary artery area, myocardial blood flow measured with radioactive microspheres to subepicardium, midmyocardium, and subendocardium did not change with the mild and severe stenosis from control. In the left anterior descending coronary artery area, myocardial blood flow to each layer remained at nearly control level with the mild stenosis but was reduced in midmyocardium and subendocardium with the severe stenosis. With the mild stenosis, diameters of coronary arterial microvessels less than 100 microns in diameter dilated, and those larger than 100 microns in diameter did not change. The magnitude of vasodilation in small arterial microvessels was inversely related to control diameter. With the severe stenosis, small arterial microvessels dilated, and simultaneously, large arterial microvessels constricted. Again, the magnitude of vasodilation in small arterial microvessels was inversely related to control diameter.(ABSTRACT TRUNCATED AT 250 WORDS)
Inhibitor Cells in Spleens of Mice With Low Natural Killer Activity&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;&lt;xref ref-type="fn" rid="FN3"&gt;3&lt;/xref&gt;
Authors: P B Blair, M O Staskawicz, J S Sam
Journal: JNCI Journal of the National Cancer Institute (1983)

Inbred strains of mice differ markedly in levels of natural killer (NK) cytotoxic activity. BPS mice normally have very low spleen cell NK activity. However, their NK activity can be significantly augmented with the use of interferon inducer, which suggests that this basic low reactivity is not a result of low numbers of precursor cells or defects in the lytic mechanism. Normally, their spleens contain suppressor cells, as evidenced by the ability of BPS spleen cells to inhibit in vitro the NK activity of spleen cells from high-responder strains of mice. Cells responsible for the suppression are located in the dense fraction after separation in Ficoll-Hypaque and are nylon wool-adherent. Studies on the kinetics of inhibition indicate that the mechanism of inhibition is not merely competition for target-binding sites. Although these suppressor cells can act on mature NK effectors in vitro, their activity in vivo may be more complex, since mature BPS NK effectors are not revealed when the suppressors are removed.
PharmGKB Submission Update: VI. PMT Submissions of Genetic Variations in Neurotransmitter Transporters (SLC6, SLC17, and SLC18) to the PharmGKB Network
Authors: Ilaria Badagnani, Marco Sorani, Robert H. Edwards, Chaline Brown, Richard A. Castro, Conrad C. Huang, Doug Stryke, Michiko Kawamoto, Susan J. Johns, Elaine J. Carlson, Travis Taylor, Wendy Chan, Melanie De La Cruz, Thomas E. Ferrin, Esteban G. Burchard, Ira Herskowitz, Deanna L. Kroetz, Kathleen M. Giacomini
Journal: Pharmacological Reviews (2006)

Category: genotype Project: Pharmacogenetics of Membrane Transporters [Table 1][1] provides HUGO Gene Nomenclature Committee (HGNC) symbols, PharmGKB submission URLs, submission dates, and release dates. [Table 2][2] provides HGNC symbols, HGNC names, synonyms, GenBank accession numbers, and locus
Corynebacterium acnes in rheumatoid arthritis. I. Isolation and antibody studies.
Authors: L E Bartholomew, F R Nelson
Journal: Annals of the Rheumatic Diseases (1972)

Numerous studies have reported the isolation of diphtheroid organisms (Corynebi various micro-organisms from patients with rheumafrom rheumatoid patients (Duthie a toid arthritis (Hill, 1968).Several unrelated organ-Alexander and others, 1968; Stev isms have been isolated including various bacteria 1969).It appears from this study thai (Schueller, 1906;Rosenow, 1914), Bedsonia isolates first appear as L-forms and f (Schachter, 1967), and mycoplasma (Arai, Ishikawa, typical bacterial or cell-wall phaw and Hotta, 1964;Bartholomew, 1965; Williams, results of complement-fixing an 1968).The isolation of diphtheroids from synovial antibody in rheumatoid patients a fluid, synovial tissue and blood in rheumatoid presented.The following paper desc arthritis has been reported (Cadham, 1942; Duthie, fication of a specific bacterial anti Stewart, Alexander, and Dayhoff, 1967; Hill, Mc-in synovial fluid leucocytes.
Interferon-α and viral triggers promote functional maturation of human monocyte-derived dendritic cells
Authors: A. Farkas, G. Tonel, F.O. Nestle
Journal: British Journal of Dermatology (2008)

Type I interferons (IFNs) play an important role in the pathogenesis of many autoimmune disorders including psoriasis. In the presence of IFN-alpha and granulocyte/macrophage colony-stimulating factor (GM-CSF), monocytes differentiate into dendritic cells (DCs) referred to as IFN-DCs. IFN-DCs potentially mimic DC populations involved in psoriasis and express a wide range of Toll-like receptor (TLR) subtypes.Recently, it was shown that single-stranded RNA (ssRNA) triggers TLR7 and TLR8; therefore we studied ssRNA, as a surrogate for ssRNA viruses and their impact on IFN-DCs.We established culture conditions for IFN-DCs, generated from plastic adherent monocytes using GM-CSF plus IFN-alpha. For DC stimulation ssRNA40, a 20-mer ssRNA oligonucleotide was used. The phenotypic analysis of DC preparations was performed using flow cytometry. The production of various cytokines was analysed by enzyme-linked immunosorbent assay, and real-time quantitative polymerase chain reaction was used to quantify TLR and cytokine gene expression. The ability of IFN-DCs to stimulate allogeneic T-cell proliferation was evaluated in a mixed leucocyte reaction.We found that IFN-DCs express mRNA for TLR7 and TLR8 and that ssRNA stimulation significantly improves their costimulatory molecule expression, stabilizes their phenotype and enhances their capacity to stimulate naive T-cell proliferation. Unstimulated IFN-DCs did not produce bioactive interleukin (IL)-12 and produced low levels of other proinflammatory cytokines. In contrast, ssRNA stimulation led to a significant production of IL-12p70, IL-1beta, IL-6 and tumour necrosis factor alpha. IFN-DCs contained mRNA for IL-12p35, IL-12p40, IL-23p19, IL-27p28 and IL-27EBI, which was further increased by incubation with ssRNA.Our study sheds light on a potential role for IFN-alpha and viral infections in triggering DC populations in psoriasis. These results provide additional data for the better understanding of human autoimmune and antiviral responses and may also have implications for strategies developing cancer immunotherapy.
Sleep quality as a confounding factor of maternal weight and preschool wheezing
Authors: E. T. M. Leermakers, A. M. M. S.-v. der Voort, R. Gaillard, A. Hofman, J. C. de Jongste, V. W. V. Jaddoe, L. Duijts
Journal: European Respiratory Journal (2014)

Considering the consequences of impaired maternal health and its implications for the child, we would like to emphasise the importance of the study by LEERMAKERS et al. [1], which evaluated maternal weight and preschool wheezing, and provides results relevant to society.Currently, weight gain is a worldwide health problem and is of great importance in pregnant females, as the health impacts can be severe, both for the mother and the neonate.We would like to ask the authors and other research groups to include sleep evaluation as a possible confounding factor, as there appears to be relationship between maternal health, obesity, sleep disorders and asthma.
Distribution of skin-derived antileucoproteases (SKALP) in the marginal zone of the spreading psoriatic lesion
Authors: P.C.M. KERKHOF, L.H.C.M. KUPPENS, Y. VLIJMEN, J. SCHALKWIJK
Journal: British Journal of Dermatology (1991)

Two new elastase inhibitors (SKALP, skin-derived antileucoproteases) were recently described in the lesional skin in psoriasis. The present study investigated the distribution of SKALP activity in the marginal zone of spreading psoriatic plaques. In a 4-mm zone immediately adjacent to the erythemato-squamous plaques, SKALP activity was slightly increased compared to distant uninvolved skin. Within the lesion the anti-elastase activity was pronounced, but was significantly higher in the central zone of the plaque compared to the periphery. The appearance of SKALP in the psoriatic lesion appears to be a late event compared to endothelial involvement, intraepidermal accumulation of PMNs, epidermal proliferation and abnormal keratinization. This observation lends further support for the hypothesis that the induction of anti-elastase activity is associated with the off-switch of cutaneous inflammation.
P777 DOSE-DEPENDENT PHARMACOKINETICS FOR MK-5172 25 mg, 50 mg, AND 100 mg ONCE-DAILY FOR 12 WEEKS IN HCV GENOTYPE 1 TREATMENT-NAIVE NON-CIRRHOTIC PATIENTS
Authors: L. Caro, J. Talaty, M. Morrison, S. Zhang, Z. Guo, I.N. Gendrano III, P. Hwang, N. Mobashery
Journal: Journal of Hepatology (2014)
The Effectiveness of Assertive Community Treatment for Homeless Populations With Severe Mental Illness: A Meta-Analysis
Authors: Craig M. Coldwell, William S. Bender
Journal: American Journal of Psychiatry (2007)

Objective: The purpose of this study was to assess the effectiveness of assertive community treatment in the rehabilitation of homeless persons with severe mental illness using a meta-analysis. Method: A structured literature search identified studies for review. Inclusion criteria were the use of an assertive community treatment-based rehabilitation treatment in an experimental or quasi-experimental model, exclusive treatment of homeless subjects, and follow-up of housing and psychiatric outcomes. Two reviewers independently abstracted data on methodology and outcomes from included studies. The authors calculated effect differences, summary effects and confidence intervals (CIs) for housing, and hospitalization and symptom severity outcomes. Results: Of the 52 abstracts identified, 10 (19%) met inclusion criteria. Of these, six were randomized controlled trials, and four were observational studies, totaling 5,775 subjects. In randomized trials, assertive community treatment subjects demonstrated a 37% (95% CI=18%–55%) greater reduction in homelessness and a 26% (95% CI=7%–44%) greater improvement in psychiatric symptom severity compared with standard case management treatments. Hospitalization outcomes were not significantly different between the two groups. In observational studies, assertive community treatment subjects experienced a 104% (95% CI=67%–141%) further reduction in homelessness and a 62% (95% CI=0%–124%) further reduction in symptom severity compared with pretreatment comparison subjects. Conclusions: Assertive community treatment offers significant advantages over standard case management models in reducing homelessness and symptom severity in homeless persons with severe mental illness.
Serological identification and cellular distribution of three F9 antigen components.
Authors: D Morello, H Condamine, C Delarbre, C Babinet, G Gachelin
Journal: The Journal of Experimental Medicine (1980)

Using an affinity chromatography technique, IgM, IgG1, IgG2a,b anti-F9 antibodies have been isolated from the anti-F9 serum; their activities have been analyzed by IF test on a variety of cell types, teratocarcinoma-derived cell lines, and embryos. The anti-F9 antibodies react with at least three independent antigenic determinants not expressed on the same cell types, and that appear along different time-course during embryonic development.
Frontiers in Nephrology
Authors: Raffaele Girlanda, Allan D. Kirk
Journal: Journal of the American Society of Nephrology (2007)

Vascularized allografts are rejected unless some indefinite modification to the recipient's immune system is made. This modification is typically achieved through the long-term administration of immunosuppressive drugs. Patients thus trade their end-stage organ failure for dependence on daily drug therapy and the accompanying chronic condition of immunodeficiency. However, it is clear from studies in experimental animals that rejection can be prevented through the use of several therapeutic approaches, including donor hematopoietic cell infusion, chimerism, T cell depletion, and/or co-stimulation blockade. Successfully treated animals avoid rejection beyond the period of therapy without a phenotype of chronic immunosuppression and are thus considered to be tolerant of their grafts. Although intriguing, this success in animals has yet to be reproducibly translated to the clinic, and human transplant recipients remain tethered to immunosuppressive drugs with rare exceptions. This article provides an overview of the existing, largely anecdotal, clinical experience with organ allograft tolerance. It reviews the various approaches that are being applied in pilot human trials and suggests avenues for future clinical investigation.