tbuckley/cabot-search huggingface dataset:
Determination of Cell Surface Membrane Antigens Common to Both Human Neuroblastoma and Leukemia-Lymphoma Cell Lines by a Panel of 38 Monoclonal Antibodies&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: Tohru Sugimoto, Eiji Tatsumi, John T. Kemshead, Lawrence Helson, Alexander A. Green, Jun Minowada
Journal: JNCI Journal of the National Cancer Institute (1984)

The surface membrane antigens of 7 neuroblastoma and 91 leukemia-lymphoma cell lines were studied with the use of a total of 36 murine monoclonal antibodies (MoAb) primarily developed against hematopoietic cells and 2 MoAb developed against human fetal brain. Five of the MoAb against hematopoietic cells (BA-1, BA-2, DU-ALL-1, J-5, and BA-3) consistently bound to common acute lymphoblastic leukemia cell lines, and 2 others (MCS-2 and OKM-1) reacted uniformly with acute myeloblastic-acute monoblastic leukemia cell lines. However, these 7 MoAb also reacted with 1-7 neuroblastoma cell lines. All the human neuroblastoma cell lines bound MoAb BA-2 and DU-ALL-1. Six of the 7 lines reacted with BA-1. Only 1 neuroblastoma cell line (SJ-N-CG) gave positive staining with J-5 and BA-3, and another line (SK-N-AS) bound MoAb MCS-2 and OKM-1. Anti-fetal brain MoAb (UJ-13A and UJ-127-11) were highly positive for all the neuroblastoma cell lines. By contrast, 4 of 43 leukemia-lymphoma cell lines tested bound these anti-fetal brain antibodies. Both B3/25 and OKT-9, anti-transferrin receptor antibodies, reacted with all of the hematopoietic and neuroblastoma cell lines. These results demonstrate that neuroblastoma and hematopoietic cell lines possess common antigenic determinants despite their different embryologic origins. The neuroblastoma cell lines may be classified into subgroups on the basis of phenotype profiles determined by the MoAb. MoAb may be useful in characterization and classification of neuroblastoma cells, as has already proved to be the case for cells of the hematopoietic lineages.
Isolation and Characterization of Nontubular Sca-1+Lin− Multipotent Stem/Progenitor Cells from Adult Mouse Kidney
Authors: Benjamin Dekel, Lior Zangi, Elias Shezen, Shlomit Reich-Zeliger, Smadar Eventov-Friedman, Helena Katchman, Jasmin Jacob-Hirsch, Ninette Amariglio, Gideon Rechavi, Raanan Margalit, Yair Reisner
Journal: Journal of the American Society of Nephrology (2006)

Tissue engineering and cell therapy approaches aim to take advantage of the repopulating ability and plasticity of multipotent stem cells to regenerate lost or diseased tissue. Recently, stage-specific embryonic kidney progenitor tissue was used to regenerate nephrons. Through fluorescence-activated cell sorting, microarray analysis, in vitro differentiation assays, mixed lymphocyte reaction, and a model of ischemic kidney injury, this study sought to identify and characterize multipotent organ stem/progenitor cells in the adult kidney. Herein is reported the existence of nontubular cells that express stem cell antigen-1 (Sca-1). This population of small cells includes a CD45-negative fraction that lacks hematopoietic stem cell and lineage markers and resides in the renal interstitial space. In addition, these cells are enriched for beta1-integrin, are cytokeratin negative, and show minimal expression of surface markers that typically are found on bone marrow-derived mesenchymal stem cells. Global gene profiling reveals enrichment for many genes downstream of developmental signaling molecules and self-renewal pathways, such as TGF-beta/bone morphogenic protein, Wnt, or fibroblast growth factor, as well as for those that are involved in specification of mesodermal lineages (myocyte enhancer factor 2A, YY1-associated factor 2, and filamin-beta). In vitro, they are plastic adherent and slowly proliferating and result in inhibition of alloreactive CD8(+) T cells, indicative of an immune-privileged behavior. Furthermore, clonal-derived lines can be differentiated into myogenic, osteogenic, adipogenic, and neural lineages. Finally, when injected directly into the renal parenchyma, shortly after ischemic/reperfusion injury, renal Sca-1(+)Lin(-) cells, derived from ROSA26 reporter mice, adopt a tubular phenotype and potentially could contribute to kidney repair. These data define a unique phenotype for adult kidney-derived cells, which have potential as stem cells and may contribute to the regeneration of injured kidneys.
Biomarker Profiling by Nuclear Magnetic Resonance Spectroscopy for the Prediction of All-Cause Mortality: An Observational Study of 17,345 Persons
Authors: Krista Fischer, Johannes Kettunen, Peter Würtz, Toomas Haller, Aki S. Havulinna, Antti J. Kangas, Pasi Soininen, Tõnu Esko, Mari-Liis Tammesoo, Reedik Mägi, Steven Smit, Aarno Palotie, Samuli Ripatti, Veikko Salomaa, Mika Ala-Korpela, Markus Perola, Andres Metspalu
Journal: PLoS Medicine (2014)

In this study, Würtz and colleagues conducted high-throughput profiling of blood specimens in two large population-based cohorts in order to identify biomarkers for all-cause mortality and enhance risk prediction. The authors found that biomarker profiling improved prediction of the short-term risk of death from all causes above established risk factors. However, further investigations are needed to clarify the biological mechanisms and the utility of these biomarkers to guide screening and prevention. Please see later in the article for the Editors' Summary
A novel anti-inflammatory drug, SDZ ASM 981, for the topical and oral treatment of skin diseases: <i>in vivo</i> pharmacology
Authors: J.G. MEINGASSNER, M. GRASSBERGER, H. FAHRNGRUBER, H.D. MOORE, H. SCHUURMAN, A. STÜTZ
Journal: British Journal of Dermatology (1997)

There is a need for safe and effective therapies for inflammatory skin diseases. Current topical and systemic treatment of psoriasis is effective but suffers from side-effects or is inconvenient. The therapeutic armamentarium for atopic dermatitis is very limited and far from satisfactory. In vivo preclinical data are presented for SDZ ASM 981, a novel ascomycin macrolactam derivative with high anti-inflammatory activity. Anti-inflammatory activity was observed in mouse, rat and pig models of allergic contact dermatitis. In the pig model, topical SDZ ASM 981 was as effective as the ultrapotent corticosteroid clobetasol-17-propionate, and when compared with a series of commercial topical corticosteroid preparations, 0.1% SDZ ASM 981 had equivalent efficacy to clobetasol-17-propionate (0.05%), the most potent product on the market. Unlike the corticosteroid, however, SDZ ASM 981 did not cause skin atrophy in pigs. SDZ ASM 981 potently inhibited allergic contact dermatitis in mice and rats when given systemically, and oral treatment was more effective than cyclosporin A in rats. Furthermore, SDZ ASM 981 has a low potential for affecting systemic immune responses, as demonstrated in rat models of localized graft vs. host reaction and allogeneic kidney transplantation. Preclinical results suggest that SDZ ASM 981 has the potential to be a well-tolerated and effective drug for topical as well as oral treatment of inflammatory skin diseases.
Increased Expression of the Chemokine Receptor CXCR3 and Its Ligand CXCL10 in Peripheral Airways of Smokers with Chronic Obstructive Pulmonary Disease
Authors: Marina Saetta, Margherita Mariani, Paola Panina-Bordignon, Graziella Turato, Cecilia Buonsanti, Simonetta Baraldo, Cinzia M. Bellettato, Alberto Papi, Lorenzo Corbetta, Renzo Zuin, Francesco Sinigaglia, Leonardo M. Fabbri
Journal: American Journal of Respiratory and Critical Care Medicine (2002)

CXCR3 is a chemokine receptor preferentially expressed on lymphocytes, particularly on type-1 T-lymphocytes. Smokers who develop chronic obstructive pulmonary disease (COPD) have a chronic bronchopulmonary inflammation that is characterized by an increased infiltration of T-lymphocytes, particularly CD8+, in the airways and lung parenchyma. To investigate the expression of CXCR3 and its ligand interferon-induced protein 10/CXCL10 in COPD, we counted the number of CXCR3+ cells and analyzed the expression of CXCL10 in the peripheral airways of 19 patients undergoing lung resection for localized pulmonary lesions. We examined lung specimens from seven smokers with fixed airflow limitation (COPD), five smokers with normal lung function, and seven nonsmoking subjects with normal lung function. The number of CXCR3+ cells was immunohistochemically quantified in the epithelium, in the submucosa, and in the adventitia of peripheral airways. The number of CXCR3+ cells in the epithelium and submucosa was increased in smokers with COPD as compared with nonsmoking subjects, but not as compared with smokers with normal lung function. Immunoreactivity for the CXCR3-ligand CXCL10 was present in the bronchiolar epithelium of smokers with COPD but not in the bronchiolar epithelium of smoking and nonsmoking control subjects. Most CXCR3+ cells coexpressed CD8 and produced interferon γ. These findings suggest that the CXCR3/CXCL10 axis may be involved in the T cell recruitment that occurs in peripheral airways of smokers with COPD and that these T cells may have a type-1 profile.
Association of Prediabetes and Diabetes With Stroke Symptoms
Authors: April P. Carson, Paul Muntner, Brett M. Kissela, Dawn O. Kleindorfer, Virginia J. Howard, James F. Meschia, Linda S. Williams, Ronald J. Prineas, George Howard, Monika M. Safford
Journal: Diabetes Care (2012)

Stroke symptoms among individuals reporting no physician diagnosis of stroke are associated with an increased risk of future stroke. Few studies have assessed whether individuals with diabetes or prediabetes, but no physician diagnosis of stroke, have an increased prevalence of stroke symptoms.This study included 25,696 individuals aged ≥ 45 years from the REasons for Geographic And Racial Differences in Stroke (REGARDS) study who reported no history of stroke or transient ischemic attack at baseline (2003-2007). Glucose measurements, medication use, and self-reported physician diagnosis were used to categorize participants into diabetes, prediabetes, or normal glycemia groups. The presence of six stroke symptoms was assessed using a validated questionnaire.The prevalence of any stroke symptom was higher among participants with diabetes (22.7%) compared with those with prediabetes (15.6%) or normal glycemia (14.9%). In multivariable models, diabetes was associated with any stroke symptom (prevalence odds ratio [POR] 1.28 [95% CI 1.18-1.39]) and two or more stroke symptoms (1.26 [1.12-1.43]) compared with normal glycemia. In analyses of individual stroke symptoms, diabetes was associated with numbness (1.15 [1.03-1.29]), vision loss (1.52 [1.31-1.76]), half-vision loss (1.54 [1.30-1.84]), and lost ability to understand people (1.34 [1.12-1.61]) after multivariable adjustment. No association was present between prediabetes and stroke symptoms.In this population-based study, almost one in four individuals with diabetes reported stroke symptoms, which suggests that screening for stroke symptoms in diabetes may be warranted.
Reperfusion Paradox in ST-Segment Elevation Myocardial Infarction
Authors: Paul W. Armstrong, William E. Boden
Journal: Annals of Internal Medicine (2011)

A transformation in ST-segment elevation myocardial infarction (STEMI) care in the United States has unfolded. It asserts superior reperfusion with primary percutaneous coronary intervention (PPCI) over fibrinolysis on the basis of studies showing the former method to be superior for reperfusion of patients with STEMI. Although clear benefit has resulted from national programs directed toward achieving shorter times to PPCI in facilities with around-the-clock access, most patients present to non-PPCI hospitals. Because delay to PPCI for most patients with STEMI presenting to non-PPCI centers remains outside current guidelines, many are denied benefit from pharmacologic therapy. This article describes why this approach creates a treatment paradox in which more effort to improve treatment for patients with PPCI for acute STEMI often leads to unnecessary avoidance and delay in the use of fibrinolysis. Recent evidence confirms the unfavorable consequences of delay to PPCI and that early prehospital fibrinolysis combined with strategic mechanical co-interventions affords excellent outcomes. The authors believe it is time to embrace an integrated dual reperfusion strategy to best serve all patients with STEMI.
Cfp1 is required for gene expression-dependent H3K4 trimethylation and H3K9 acetylation in embryonic stem cells
Authors: Thomas Clouaire, Shaun Webb, Adrian Bird
Journal: Genome biology (2014)

Abstract Background Trimethylation of histone H3 lysine 4 (H3K4me3) accumulates at promoters in a gene activity-dependent manner. The Set1 complex is responsible for most H3K4me3 in somatic cells and contains the conserved subunit Cfp1, which is implicated in targeting the Set1 complex to CpG islands in mammals. In mouse embryonic stem cells, Cfp1 is necessary for H3K4me3 accumulation at constitutively active gene promoters, but is not required to maintain steady-state transcription of the associated gene. Results Here we show that Cfp1 is instrumental for targeting H3K4me3 to promoters upon rapid transcriptional induction in response to external stimuli. Surprisingly, H3K4me3 accumulation is not required to ensure appropriate transcriptional output but rather plays gene-specific roles. We also show that Cfp1-dependent H3K4me3 deposition contributes to H3K9 acetylation genome-wide, suggesting that Cfp1-dependent H3K4me3 regulates overall H3K9 acetylation dynamics and is necessary for histone acetyl transferase recruitment. Finally, we observe increased antisense transcription at the start and end of genes that require Cfp1 for accurate deposition of H3K4me3 and H3K9ac. Conclusions Our results assign a key role for Cfp1 in establishing a complex active promoter chromatin state and shed light on how chromatin signaling pathways provide context-dependent transcriptional outcomes.
Evidence for Recombination of mtDNA in the Marine Mussel Mytilus trossulus from the Baltic
Authors: A. Burzynski
Journal: Molecular Biology and Evolution (2003)

A number of studies have claimed that recombination occurs in animal mtDNA, although this evidence is controversial. Ladoukakis and Zouros (2001) provided strong evidence for mtDNA recombination in the COIII gene in gonadal tissue in the marine mussel Mytilus galloprovincialis from the Black Sea. The recombinant molecules they reported had not however become established in the population from which experimental animals were sampled. In the present study, we provide further evidence of the generality of mtDNA recombination in Mytilus by reporting recombinant mtDNA molecules in a related mussel species, Mytilus trossulus, from the Baltic. The mtDNA region studied begins in the 16S rRNA gene and terminates in the cytochrome b gene and includes a major noncoding region that may be analogous to the D-loop region observed in other animals. Many bivalve species, including some Mytilus species, are unusual in that they have two mtDNA genomes, one of which is inherited maternally (F genome) the other inherited paternally (M genome). Two recombinant variants reported in the present study have population frequencies of 5% and 36% and appear to be mosaic for F-like and M-like sequences. However, both variants have the noncoding region from the M genome, and both are transmitted to sperm like the M genome. We speculate that acquisition of the noncoding region by the recombinant molecules has conferred a paternal role on mtDNA genomes that otherwise resemble the F genome in sequence
The prospective association between psychological distress and disease activity in rheumatoid arthritis: a multilevel regression analysis
Authors: Cécile L Overman, Ercolie R Bossema, Henriët van Middendorp, Leoniek Wijngaards-de Meij, Suzanne MM Verstappen, Marcia Bulder, Johannes WG Jacobs, Johannes WJ Bijlsma, Rinie Geenen
Journal: Annals of the Rheumatic Diseases (2011)

<h3>Background</h3> Cross-sectional associations suggest a mutual impact of disease activity and psychological distress in rheumatoid arthritis (RA), but a prospective association has not been established. <h3>Objective</h3> To examine concurrent and prospective associations between psychological distress and disease activity. <h3>Methods</h3> Patients with RA (N=545, disease duration ≤1 year, age 18–83 years, 69% female, 64% rheumatoid factor (RF) positive) were monitored for 5 years. The Thompson joint score and erythrocyte sedimentation rate were assessed every 6 months. Depressed mood and anxiety were measured every 12 months. Multilevel regression analysis was used. RF positivity, age and female sex were included as covariates. <h3>Results</h3> Concurrent levels of psychological distress and disease activity were positively associated (p≤0.04). Prospectively, depressed mood was associated with disease activity levels 6 months later (p≤0.04). The Thompson joint score was associated with psychological distress levels 6 months later (p≤0.03) and also with an increase in depressed mood over the subsequent 6 months (p=0.02). No other significant prospective associations were found (p≥0.07). <h3>Conclusions</h3> Psychological distress and disease activity are positively associated when measured at the same time as well as when measured 6 months apart. While some support was found for the idea that a higher level of disease activity is a risk factor for an increase in psychological distress, the results do not support the notion that psychological distress is a risk factor for future exacerbation of disease activity.
Postnatal lung growth after repair of diaphragmatic hernia.
Authors: W M Thurlbeck, K Kida, C Langston, M J Cowan, J A Kitterman, W Tooley, H Bryan
Journal: Thorax (1979)

The lungs of two patients who died eight months and 64 months after repair of a left-sided diaphragmatic hernia on the first day of life were examined. Lung volumes were appropriate for the size of the children, and the ratio of right lung volume to left lung volume was also normal in both patients. The lungs, however, were grossly abnormal with evidence of enlargement and destruction of respiratory tissue. The left lung was affected more than the right in both subjects. In one patient the total number of alveoli in the lungs was similar to that of normal children of the same age, but this was because the right lung had more than twice as many alveoli as the left lung. It appears that alveolar multiplication is impaired after repair of diaphragmatic hernia. The number of bronchioles was reduced in the infant with very few alveoli, and there may have been too few bronchioles in the other patient.
The Missing Piece
Authors: Kathryn B. Robertson, William J. Janssen, Sanjay Saint, Steven E. Weinberger
Journal: New England Journal of Medicine (2006)

A 21-year-old man presented to the emergency department after 2 days of pain in the right lower abdominal quadrant. The pain was dull and nonradiating, and it had increased in intensity during those 2 days. It was not worsened by food intake and was unrelated to bowel movements. He also noted that for the past month he had had exertional dyspnea with a cough productive of scanty yellow sputum.
Referral Failures: A One-Year Follow-Up
Authors: WALTER A. CHAMEIDES, JOE YAMAMOTO
Journal: American Journal of Psychiatry (1973)

The authors found that most of the patients in their study—98 out of 153—did not make or keep their appointments at an outpatient clinic after being evaluated and referred. One year later they were able to obtain information on 51 of these patients and found that 40 of them had received some type of professional care.
The Application of Esophageal Pressure Measurement in Patients with Respiratory Failure
Authors: Evangelia Akoumianaki, Salvatore M. Maggiore, Franco Valenza, Giacomo Bellani, Amal Jubran, Stephen H. Loring, Paolo Pelosi, Daniel Talmor, Salvatore Grasso, Davide Chiumello, Claude Guérin, Nicolo Patroniti, V. Marco Ranieri, Luciano Gattinoni, Stefano Nava, Pietro-Paolo Terragni, Antonio Pesenti, Martin Tobin, Jordi Mancebo, Laurent Brochard
Journal: American Journal of Respiratory and Critical Care Medicine (2014)

Section:ChooseTop of pageAbstract <<Physiological BackgroundClinical Use of Esophagea...Clinical Use of Esophagea...TechniqueConclusionsReferencesCITING ARTICLES
Total haemoglobin mass and selected anthropometric parameters in male and female judokas
Authors: J Malczewska-Lenczowska, D Sitkowski, A Pokrywka, W Błach, A Maj, W Borowiak
Journal: British Journal of Sports Medicine (2011)

Total haemoglobin mass (tHb-mass) is one of the most important factors contributing to aerobic performance. Its level strongly depends on body size, so it is usually expressed in relation to kg body mass (BM), but some studies in male endurance athletes have shown that lean body mass (LBM) is a more appropriate reference for expression of tHb-mass. The aim of this study was to determine relationships between tHb-mass and selected anthropometric reference parameters in a discipline with diverse body mass. 43 highly trained judokas (28 males and 15 females) underwent tHb-mass determination (optimised CO rebreathing method) and anthropometric evaluation (skinfold, body mass and height measurement) with estimation of lean body mass (LBM), and body surface area (BSA). Linear regression analysis between tHb-mass and anthropometric indices was performed, and Pearson's coefficients of correlation were calculated. Influence of gender on the tHb-mass/BM, /LBM and /BSA relationships was tested by analysis of covariance (ANCOVA). Gender differences in tHb-mass expressed in relation to this parameters were tested by Student's t-test (significance level p&lt;0.05). BM values were in the range 59.8–169.0 kg in male judokas and 48.8–170.0 kg in females. In both groups, tHb-mass correlated with BM (r=0.82, r=0.92), LBM (r=0.86, r=0.96), and BSA (r=0.88, r=0.94) in male and female judokas, respectively. Mean values of tHb-mass in male and female athletes were 11.1±1.1 and 8.8±1.3 g/kg BM (p&lt;0.0001), respectively. This difference was smaller, but still statistically significant when tHb-mass was divided by LBM (12.8±1.0 and 11.9±0.9 /kg LBM, p&lt;0.01) or BSA (470±40 and 335±23 /m 2 ; p&lt;0.001). ANCOVA showed that gender has a significant influence on tHb-mass not only after normalisation for BM (p&lt;0.001) but also for LBM (p&lt;0.01) and BSA (p&lt;0.001). Gender has a significant influence on tHb-mass even when expressed in relation to LBM or BSA, probably due to gender differences in the fraction of skeletal muscle mass in LBM.
Monitoring Serial Changes in Circulating Human Breast Cancer Cells in Murine Xenograft Models
Authors: Jean-Pierre Eliane, Madeline Repollet, Kathryn E. Luker, Martha Brown, James M. Rae, Gabriela Dontu, Anne F. Schott, Max Wicha, Gerald V. Doyle, Daniel F. Hayes, Gary D. Luker
Journal: Cancer Research (2008)

Abstract Circulating tumor cells (CTC) are emerging as a powerful prognostic and predictive biomarker in several types of cancer, including breast, colon, and prostate. Studies of CTC in metastasis and further development of CTC as a biomarker in cancer have been limited by the inability to repetitively monitor CTC in mouse models of cancer. We have validated a method to enumerate CTC in blood samples obtained from living mice using a modified version of an in vitro diagnostic system for quantifying CTC in patients. Different routes of blood collection were tested to identify a method to reproducibly recover CTC from tumor-bearing mice without interference from contaminating normal murine epithelial cells. CTC are present in blood samples from mice bearing orthotopic xenografts of several different breast cancer cell lines and primary breast cancer cells from patient biopsies. We also show that this technology can be used for serial monitoring of CTC in mouse xenograft models of human breast cancer. These results establish a new method for studying CTC in mouse models of epithelial cancer, providing the foundation for studies of molecular regulation of CTC in cancer and CTC as biomarker for therapeutic efficacy. [Cancer Res 2008;68(14):5529–32]