tbuckley/cabot-search huggingface dataset:
Abstract 286: Myocardin-related Transcription Factors Control Epicardial EMT and Coronary Vessel Homeostasis
Authors: Michael A Trembley, Lissette S Velasquez, Corey M Hoffman, Karen L de Mesy Bentley, Eric M Small
Journal: Circulation Research (2014)

The epicardium is a source of multi-potent progenitor cells that envelop the heart and contribute to various cardiac lineages through the process of epithelial-to-mesenchymal transition (EMT). Although known upstream cues promote epicardial EMT via changes in gene expression and actin cytoskeletal dynamics, the link between these signals and progenitor cell motility remain unclear. Myocardin-related transcription factor (MRTF) cofactors are primarily retained in the cytoplasm through interactions with G-actin. However, upon cytoskeletal reorganization and G-actin depletion, nuclear MRTFs associate with serum response factor (SRF) to drive cellular motility and contractility gene programs reminiscent of EMT. Here, we identify the SRF/MRTF gene regulatory axis as a key mediator of epicardial EMT. We found significant enrichment of MRTF-A and -B in the epicardium prior to EMT. MRTFs were later expressed in a spatial and temporal manner concurrent with EMT and epicardial-derived cell (EPDC) differentiation. Furthermore, MRTF deletion attenuates contractile gene expression in epicardial explants and impairs migration of EPDCs into subjacent cell layers using ex vivo assays. Epicardial EMT and EPDCs differentiation are necessary for proper coronary vessel formation. We found that genetic ablation of MRTFs results in disrupted coronary plexus formation, endothelial cell dysfunction, and sub-epicardial hemorrhage. The vascular phenotype observed in MRTF-A/B epiDKO mice results in part from the depletion (~50% reduction) of epicardial-derived coronary pericytes. These data suggest a critical role for MRTFs in coronary vessel formation by regulating epicardial EMT and mobilizing EPDCs.
Reporting of clinical trials.
Authors: D Pinkel, E Gehan
Journal: Journal of Clinical Oncology (1990)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1990.8.1.180 Journal of Clinical Oncology - published online before print September 22, 2016 PMID: 2295909 Reporting of clinical trials. D PinkelxD PinkelSearch for articles by this author , E GehanxE GehanSearch for articles by this author https://doi.org/10.1200/JCO.1990.8.1.180 First Page PDF © 1990 by American Society of Clinical Oncology
Inefficient Humoral Immune Response of Lymphoma-Prone Wild Mice to Persistent Leukemia Virus Infection2
Authors: Václav Klement, Murray B. Gardner, Brian E. Henderson, James N. lhle, John D. Estes, Allan G. Stanley, Raymond V. Gilden
Journal: JNCI Journal of the National Cancer Institute (1976)

Adult wild mice (LC) from a natural colony with a high incidence of spontaneous lymphomas had free infectious virus in their sera (average 103.5 infectious units/ml) and parenchymal organs (average 105.2 infectious units/g tissue). They did not have detectable levels of free virus-specific antibodies that could be demonstrated by virus neutralization or immunofluorescence at higher than a 1:10 dilution. Only 5 of 28 animals had free antibodies detectable by radioimmunoprecipitation assay, and tissues of 4 mice also had nondetectable levels of virus determined by infectivity assay. Formalized vaccine from the indigenous virus did not induce production of virus-neutralizing antibodies or protect against naturally occurring disease. The animals with persistent leukemia virus infection, however, elicited good humoral immune responses to virus-unrelated antigen.
Abstract P293: Post-Traumatic Stress Disorder, Exposure to Particular Matter and its Cardiovascular Implications
Authors: Rupa L Iyengar, Cynara Maceda, Jacqueline O'Boyle, Heather Beebe, Yuliya Yakunina, Simonette T Sawit, Laura E Crowley, Mark Woodward, Mary Ann McLaughlin
Journal: Circulation (2014)

Background: Exposure to particulate matter (PM) is a risk factor for cardiovascular disease (CVD). It is likely that different mechanisms, including inflammatory processes, are responsible for acute and chronic toxic effects. First responders during the World Trade Center (WTC) tragedy have been exposed to PM. Post-Traumatic Stress Disorder (PTSD) is well-established in the WTC cohort. High sensitivity C-reactive protein (hsCRP) is a marker of inflammtion and is indicative of increased CVD risk. In this abstract we aim to investigate the relationship between PTSD and PM exposure and its impact on CVD. Methods: We evaluated 816 participants in the World Trade Center-CHEST Program from January 2011 to September 2013. PTSD was defined as self-reported diagnosis and/or a score of above 50 on the PTSD questionnaire. Using the Wisnevsky’s exposure score, participants were classified into four exposure groups, very high, high, intermediate and low. Chi-square analysis, independent t-test, and linear regressions were performed to determine if there was any significant relationship between PTSD, Exposure and CVD. Results: Participants with very high or high exposure score were more likely to have PTSD (p=0.001). Those with PTSD had higher hsCRP which were trending to significance (p=0.053). When adjusted further for CVD risk and exposure, hsCRP was significantly related to PTSD (p = 0.044). Conclusions: PTSD is significantly related to PM exposure in this cohort of WTC responders. PTSD is a condition that not only affects level of function on a psychological level but can also have an impact on CVD health. PTSD and PM exposure should be considered when assessing CVD risk.
Abstract 14745: Chemotherapy Causes Diastolic More Than Systolic Dysfunction Without a Change in Myocardial Tissue Characteristics
Authors: Yasmin S Hamirani, Roni Jiji, Michael Salerano, Patrick Dillon, Christiana Brenin, Adrian I Loffler, Andrew H Wong, Jennifer Kay, John Christopher, Christopher M Kramer
Journal: Circulation (2013)

BACKGROUND: Anthracyclines can cause cardiotoxicity and heart failure but predictors of a decline in ejection fraction (EF) and diastolic dysfunction are lacking. . We hypothesized that changes in ...
Cell-Mediated Cytotoxicity Against HeLa Cells in Patients With Invasive or Preinvasive Cervical Cancer 2
Authors: Eero Saksela, Bengt Meyer
Journal: JNCI Journal of the National Cancer Institute (1973)

Journal Article Cell-Mediated Cytotoxicity Against HeLa Cells in Patients With Invasive or Preinvasive Cervical Cancer Get access Eero Saksela, Eero Saksela 3III Department of Pathology, University of Helsinki, Helsinki 29, Finland Search for other works by this author on: Oxford Academic PubMed Google Scholar Bengt Meyer Bengt Meyer 4Departments of Obstetrics and Gynecology, University Central Hospital, Helsinki 29, Finland Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 51, Issue 4, October 1973, Pages 1095–1102, https://doi.org/10.1093/jnci/51.4.1095 Published: 01 October 1973 Article history Received: 26 February 1973 Accepted: 07 June 1973 Published: 01 October 1973
Spontaneous Feline Fibrosarcomas: Transmissibility and Ultrastructure of Associated Virus-Like Particles&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: S. P. Snyder
Journal: JNCI Journal of the National Cancer Institute (1971)

Electron microscopic examination of tumor tissue from 7 cats with spontaneous fibrosarcomas revealed the presence of C-type virus particles in tumors from 3 animals. Five newborn kittens were inoculated with cell-free materials prepared from each of the 3 virus-positive tumors and an additional fibrosarcoma in which no virus particles were noted. Of the 20 kittens inoculated with the 4 preparations, 17 developed fibrosarcomas, which indicated that all 4 spontaneous tumors tested were transmissible. Morphologically similar virus particles were found in all experimentally induced tumors examined. C-type particles, approximately 115 mµ, in diameter, budded from the plasma membrane and into membrane-bound vacuoles in the cytoplasm of tumor cells. Both immature and mature forms of the virion were found free in extracellular spaces.
Simultaneous Ex Vivo Expansion of Cytomegalovirus and Epstein-Barr Virus–Specific Cytotoxic T Lymphocytes Using B-Lymphoblastoid Cell Lines Expressing Cytomegalovirus pp65
Authors: Qi Sun, Karen E. Pollok, Robert L. Burton, Li Jun Dai, William Britt, David J. Emanuel, Kenneth G. Lucas
Journal: Blood (1999)
Exploitation of the Apoptosis-Primed State of MYCN-Amplified Neuroblastoma to Develop a Potent and Specific Targeted Therapy Combination
Authors: Jungoh Ham, Carlotta Costa, Renata Sano, Timothy L. Lochmann, Erin M. Sennott, Neha U. Patel, Anahita Dastur, Maria Gomez-Caraballo, Kateryna Krytska, Aaron N. Hata, Konstantinos V. Floros, Mark T. Hughes, Charles T. Jakubik, Daniel A.R. Heisey, Justin T. Ferrell, Molly L. Bristol, Ryan J. March, Craig Yates, Mark A. Hicks, Wataru Nakajima, Madhu Gowda, Brad E. Windle, Mikhail G. Dozmorov, Mathew J. Garnett, Ultan McDermott, Hisashi Harada, Shirley M. Taylor, Iain M. Morgan, Cyril H. Benes, Jeffrey A. Engelman, Yael P. Mossé, Anthony C. Faber
Journal: Cancer Cell (2016)

Fewer than half of children with high-risk neuroblastoma survive. Many of these tumors harbor high-level amplification of MYCN, which correlates with poor disease outcome. Using data from our large drug screen we predicted, and subsequently demonstrated, that MYCN-amplified neuroblastomas are sensitive to the BCL-2 inhibitor ABT-199. This sensitivity occurs in part through low anti-apoptotic BCL-xL expression, high pro-apoptotic NOXA expression, and paradoxical, MYCN-driven upregulation of NOXA. Screening for enhancers of ABT-199 sensitivity in MYCN-amplified neuroblastomas, we demonstrate that the Aurora Kinase A inhibitor MLN8237 combines with ABT-199 to induce widespread apoptosis. In diverse models of MYCN-amplified neuroblastoma, including a patient-derived xenograft model, this combination uniformly induced tumor shrinkage, and in multiple instances led to complete tumor regression.
Presence of Two Cytogenetic Forms of Amplified DNA: Evidence for a Role in Tumor Growth in an Intraspecific Mouse Hybrid Cell Line
Authors: Caroline B. Wigley, John K. Cowell
Journal: JNCI Journal of the National Cancer Institute (1984)

An intraspecific mouse hybrid epithelial cell line, F5/B, is described in which the homogeneously staining region (HSR)-containing marker chromosome from one parent is absent in about half of the cells. It is replaced in these cells by double minutes (DM), an alternative form of amplified DNA, which is liable to loss because of its instability at mitosis. DM probably arise from the breakdown of the HSR during clonal growth of F5/B. Subclones were derived possessing one or another cytogenetic feature, and their cloning efficiency in vitro and tumorigenicity in syngeneic animals were compared. There were no differences in in vitro tumorigenicity, but in vivo DM-containing subclones were significantly less tumorigenic than HSR-containing subclones or the F5/B parent hybrid. In tumors that developed after long latent periods, cells had increased numbers of DM compared with the inoculated population, demonstrating a selective advantage in vivo for cells with a high DM content. These results indicate a role for the amplified DNA in tumor growth.
Treatment of metastatic retinoblastoma with paclitaxel. Preliminary results of a pilot study with seven patients
Authors: C. A. Leal-Leal, R. Rivera-Luna, M. E. Flores-Rojo, J. Amador -Zarco, C. Juarez-Echenique
Journal: Journal of Clinical Oncology (2004)

8569 Background: Metastatic retinoblastoma has a very high mortality rate in most of the cases in spite that international efforts have been made with different chemotherapy regimens. Only high doses of chemotherapy followed by steam cells rescue have proved to provide long lasting survival. The purpose of the current work is to present a preliminary report in a small number of patients. Methods: In a prospective study patients with pathological diagnosis of metastatic retinoblastoma were included, regardless of having recurrent disease or metastasis at time of diagnosis. Once the staging procedure with imaging was performed, the patients were treated with paclitaxel as a single agent at a dose of 150 mg/m2 days 1, 4 and 7 days every 21 days from 2–6 courses. Results: . At the present time 7 patients were registered into the study. Their age ranged from 10 to 60 months old with a mean of 36 months and SD of 15 months. Six were boys and 1 girl. The time prior to develop metastasis was 11 months and a SD of 13 months. No patient presented toxicity effects. All patients presented complete remission as per CAT scan and MRI studies by the second chemotherapy cycle, even intraocular dieses went to ptisis bublbi. By the 20th month of follow up all children have died with tumor activity. Conclusions: In this pilot study, placlitaxel showed to be an effective drug against intraocular and metastatic Retinoblastoma. However, it should be tested in combination with other drugs in order to obtain long lasting survival for affected patients. No significant financial relationships to disclose.
Abstract 11882: Comprehensive Hemodynamic Analysis of Coronary Arterial Responses to Acetylcholine Provocation Test in Patients With Coronary Vasospastic Angina
Authors: Kenshi Yamanaga, Kenichi Tsujita, Naohiro Komura, Kenji Sakamoto, Masanobu Ishii, Noriaki Tabata, Takashi Miyazaki, Tomonori Akasaka, Yuichiro Arima, Takamichi Ono, Sunao Kojima, Shinji Tayama, Koichi Kaikita, Seiji Hokimoto, Sunao Nakamura, Hisao Ogawa
Journal: Circulation (2014)

Introduction: Whereas our prior study showed diffuse coronary intimal thickening in pts with coronary vasospastic angina (VSA), coronary epicardial and microvascular (MV) hemodynamic and vessel response during coronary vasospasm has not been well studied. Hypothesis: We hypothesized that coronary pathogenic abnormalities responsible for coronary vasospasm may not be confined to coronary epicardial spastic segments, but the hemodynamic abnormalities may exist in the entire coronary bed. Methods: According to acetylcholine (ACh) provocation tests, excluding pts with atherosclerotic coronary obstruction (stenosis &gt;50% diameter) and possible heart failure (left ventricular ejection fraction &lt;50%), 41 pts were divided into 2 groups; VSA group (n=20) and non-VSA group (n=21). To evaluate hemodynamic parameters comprehensively, average peak velocity (APV), coronary blood flow (CBF: π (APV/2)(vessel diameter/2) 2 ), hyperemic MV resistance (hMR), coronary vascular resistance (CVR: Pd/CBF) and coronary distal pressure (Pd) were measured continuously utilizing a dual-sensor (Doppler velocity and pressure)-equipped guidewire (Combowire, Volcano Therapeutics, Inc.) during ACh provocation test. Results: There were no differences between the 2 groups in baseline characteristics. In spite of absence of between-group differences at baseline in CBF (55±26 vs. 51±29, p=0.5) and CVR (2.4±1.5 vs. 2.6±1.4, p=0.7), intracoronary ACh 100μg provocation significantly increased CVR (2.1±2.2 vs. 0.8±0.7; p=0.001), and then attenuated CBF (77±54 vs. 188±129, p=0.001) in VSA group than in non-VSA group. Furthermore, although coronary flow reserve was identical between the groups (2.2±0.9 [VSA] vs. 2.4±0.9 [non-VSA], p=0.5), hMR was significantly augmented in VSA group (2.0±0.7 vs. 1.6±0.6, p=0.046). Also, there was significantly negative correlation between hMR and estimated glomerular filtration rate, which presumably reflect the integrity of renal MV bed (r=-0.522; p=0.0009). Conclusions: Compared with non-VSA pts, coronary and systemic MV function was impaired in pts with VSA. In vivo assessment using Combowire clarified dynamic entire coronary alteration during ACh provocation test, including coronary MV dysfunction, in pts with VSA.
Isolation of &lt;italic&gt;Herpesvirus saimiri&lt;/italic&gt; From Blood of Squirrel Monkeys (&lt;italic&gt;Saimiri sciureus&lt;/italic&gt;)&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: Falk, Lawrence A., Wolfe, Lauren G., Deinhardt, Freidrich
Journal: JNCI Journal of the National Cancer Institute (1972)

Virus was isolated from 11 of 13 blood samples obtained from healthy squirrel monkeys (Saimiri sciureus); these isolates were antigenically similar, if not identical, to Herpesvirus saimiri (HVS). By co-cultivation with vero cells, virus was isolated from both whole blood and lymphocytes separated from whole blood on Ficoll-Hypaque (FH) gradients. Attempts to isolate virus from FH cell pellets (erythrocytes and polymorphonuclear leukocytes) or cell-free extracts of whole blood, lymphocytes or cell pellets failed. The squirrel monkey isolates were neutralized or reacted positively in immunofluorescence tests with anti-HVS sera but not with antisera to herpes simplex virus or Herpesvirus platyrrhinae. Cotton-topped (Saguinus oedipus) and white-lipped (S. fuscicollis and S. nigricollis) marmosets, experimentally infected with 1 isolate, developed a lympho-proliferative disease and survived 18–26 days post inoculation. Macroscopic and microscopic features of the neoplastic disease were indistinguishable from those produced by the prototype strain of HVS.
Compliance (COMP) with colon cancer (CoC) national guidelines (NG) for chemotherapy (CT) by implementing electronic medical records (EMR)
Authors: B. Ebrahimi, C. A. Presant, L. D. Bosserman, R. Horns, M. Vakil, G. Upadhyaya, C. Yeon, F. Howard, W. McNatt, B. Emilio
Journal: Journal of Clinical Oncology (2008)

17527 Background: Quality of care (QC) improves when NG are followed in patient (pt) management. Demonstration of COMP with NG is costly and slow (QOPI, and ASCO 2007 abstract 17050). We extended our prior experience to apply an EMR to monitor our pts for COMP with NCCN CoC CT guidelines. Methods: An EMR was implemented in 2003 in Wilshire Oncology Medical Group (6 CT sites and 8 medical oncologists (MO)). All pts seen in the offices were entered into the EMR by MO (ICD9 diagnosis (DX), stage (STG), history, orders) and staff (CT given). All pts were entered into a separate nonintegrated billing system (BIS) by staff. All pts with CoC seen between 6–1-06 and 5–31- 07 were evaluated electronically for DX, CT, and STG using EMR and checked in BIS. To study changes in COMP with NCCN NG for CT over time, 2 cohorts of pts were compared: pts initially seen prior to 6–1-07 (PRIOR); versus pts new after 6–1-07 (NEW). Results: Of 345 CoC pts seen by MO, 78 (25%) were seen in hospital only and not followed in office due to insurance, transfer of care, hospice, or no show. 33 (9.6%) had an incorrect ICD9 DX by MO or staff. Of 234 CoC pt in office, no STG was entered in 15 (6.4%, range per MO 0% to 13%). This was more frequent in PRIOR pt (8.9%) than NEW pt (2.3%, p=0.05). Pts were STG 0 2%, STG 1 12%, STG 2 22%, STG 3 21%, and STG 4 13%. CT was given to 0% STG 0, 3% STG 1, 24% STG 2, 29% STG 3 and 78% STG 4. CT improved by NG from PRIOR to NEW pt: STG 1 4% PRIOR to 0% NEW; STG 2 12% PRIOR to 44% NEW (p=0.02); STG 3 20% PRIOR to 52% NEW (p=0.008); but no change in STG 4 (81% to 75%, p=0.6). CT for STGs 2,3, or 4 increased from 28% to 53.6% (p=0.008), and varied by MO for NEW pt from 33% to 71%. Evaluation of reason for non-COMP with NG could not be evaluated electronically using this EMR due to lack of explanatory codes. Conclusions: EMRs can be used to monitor COMP with NG, track improvement over time, and evaluate variances in COMP between MO. MO completion of EMR data elements improves with time, but EMR data sets must be frequently monitored for completeness and accuracy by MO. EMRs should be integrated with BIS to avoid incorrect DX. Monitoring lack of 100% COMP with NG requires an additional explanatory code in EMR. QC can be improved by appropriate EMR use. This EMR has been updated after 6–07 to implement these conclusions. No significant financial relationships to disclose.
P-217 The use of neo-adjuvant chemotherapy for locally advanced colon cancer in the Netherlands
Authors: M. Verstegen, J.M. Gooyer, J. t'Lam-Boer, S. Radema, M. Elferink, A. ten Tije, C. Verhoef, A. Rijken, J.M.J. Schreinemakers, J. De Wilt
Journal: Annals of Oncology (2015)
N-of-1 approach to determine when adverse effects are caused by statins
Authors: Shah Ebrahim, George Davey Smith
Journal: BMJ (2015)

McPherson finds it shocking that a Cochrane review group is not interested in seeking data on adverse events associated with statins.1 If he read our Cochrane reviews on statins he would see that findings related to adverse events are reported.2 What is more shocking is that a systematic review of the adverse effects of statins using observational and randomised trial evidence that we submitted to The BMJ …
Healthcare-associated Crimean-Congo haemorrhagic fever in Turkey, 2002–2014: a multicentre retrospective cross-sectional study
Authors: H. Leblebicioglu, M. Sunbul, R. Guner, H. Bodur, C. Bulut, F. Duygu, N. Elaldi, G. Cicek Senturk, Z. Ozkurt, G. Yilmaz, T.E. Fletcher, N.J. Beeching
Journal: Clinical Microbiology and Infection (2016)

Healthcare-related transmission of Crimean-Congo haemorrhagic fever (CCHF) is a well-recognized hazard. We report a multicentre retrospective cross-sectional study undertaken in Turkey in 2014 in nine hospitals, regional reference centres for CCHF, covering the years 2002 to 2014 inclusive. Data were systematically extracted from charts of all personnel with a reported health care injury/accident related to CCHF. Blood samples were tested for CCHF IgM/IgG by enzyme-linked immunosorbent assay and/or viral nucleic acid detection by PCR after the injury. Fifty-one healthcare-related exposures were identified. Twenty-five (49%) of 51 resulted in laboratory-confirmed infection, with a 16% (4/25) overall mortality. The main route of exposure was needlestick injury in 32/51 (62.7%). A potential benefit of post-exposure prophylaxis with ribavirin was identified.
Type B and Type C RNA Tumor Virus Replication in Single Cells. Electron Microscopy With Tannic Acid2
Authors: G. Schidlovsky, G. P. Shibley, C. V. Benton, J. E. Elser
Journal: JNCI Journal of the National Cancer Institute (1978)

Simultaneous replication of murine mammary tumor virus (type B) and murine leukemia virus (type C) was demonstrated electron microscopically along continuous stretches of the plasma membrane of single cells in cultures of the Mm5mt/C1 cell line. Types B and C virus buds were discriminated in thin sections with the aid of a tannic acid fixative that revealed the type B surface spikes as a homogeneous band of intermediate density and constant width on the surfaces of some buds (type B), whereas others (type C) remained with relatively smooth envelopes. Both types B and C buds may contain morphologically identical horseshoe-shaped nucleoids. Therefore, their identify (type B or C) could be ascertained in thin sections only on the basis of recognition of surface spikes.