tbuckley/cabot-search huggingface dataset:
FRI0037 A Comparative Study of Ultrasonography of Interphalangeal Joints in Rheumatoid Arthritis Patients in Remission and in Healthy Subjects
Authors: K. Ben Abdelghani, S. Miladi, L. Souabni, S. Kassab, S. Chekili, A. Laatar, L. Zakraoui
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Musculoskeletal Ultrasound (US) is now becoming part of the daily practice of rheumatology. In fact, it is a useful tool in rheumatoid arthritis (RA) for detecting persisting infra-clinical synovitis in patients in remission. However, possible existence of a physiological effusion of the PIP was demonstrated in healthy subjects. <h3>Objectives</h3> The purpose of our study was to assess the presence of US effusion in proximal interphalangeal (PIP) joints of RA patients in remission and to compare it to healthy controls. <h3>Methods</h3> Thirty RA patients in remission (Disease Activity Score 28 joints &lt;2,6 for at least 3 months) and 17 healthy control patients were prospectively enrolled. All of the patients had a musculoskeletal ultrasound (US) examination of the PIP of both hands from the second to the fifth. US (Esaote MyLab 60 machine and a 13-18 MHz linear array transducer) was performed by a rheumatologist with theoretical and practical training in musculoskeletal ultrasonography. The findings obtained by grey scale (GS) and PD were graded on a semi-quantitative scale from 0 to 3. <h3>Results</h3> The mean age of RA patients in remission was 48 years-old and for control group 45 years-old. For the 240 joints scanned of RA patients, 37 (15%) joints presented synovitis in B mode and 9 (4%) a PD activity. Concerning control group, 136 joints were examined. Among them, 57 (42%) joints expressed synovitis in B mode and none in PD. The mean US score was 0,2 for RA patients and 0,5 for control group. The difference between two groups was significant (p=0,04). <h3>Conclusions</h3> US activity can be detected in B mode but not in PD in PIP even in healthy subjects. Synovitis detected in PIP in RA patients even in remission must be taken into account when assessing the activity of the RA in order to avoid further damages and disabilities. <h3>References</h3> Rosenberg C. High frequency of ultrasonographic effusion in interphalangeal joints of healthy subjects: A descriptive study. BMJ 2009 Witt M. Relevance of grade 1 gray-scale ultrasound findings in wrists and small joints to the assessment of subclinical synovitis in rheumatoid arthritis. Arthritis Rheum. 2013 <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.5224
Surgical Closure of Atrial Septal Defect in Adults
Authors: Joseph K. Perloff
Journal: New England Journal of Medicine (1995)

Leonardo da Vinci's description in 1513 of a “perforating channel” in the atrial septum is believed to be the first recorded account of a congenital malformation of the human heart.1 Atrial septal defects often go unrecognized for decades, because there are no symptoms and because the physical signs (such as a soft pulmonary-flow murmur and fixed splitting of the second heart sound) are subtle. Survival into adulthood is the rule, and patients sometimes live to advanced ages.27 Indeed, the renowned cardiologist Paul Wood remarked, “In any series of geriatric necropsies atrial septal defect is always represented.”8 Life expectancy is . . .
OBTENCIÓN DEL ÍNDICE Ki-67 EN TUMORES NEUROENDOCRINOS PANCREÁTICOS MEDIANTE USE-PAAF CON AGUJAS FINAS: 22 Y 25 G
Authors: S Salord, JB Gornals, I Català, N Peláez, N Baixeras, C Consiglieri, R Llatjós, T Serrano, J Busquets, J Fabregat
Journal: Endoscopy (2014)

Introducción: El Ki-67 es un factor pronóstico en los tumores pancreáticos neuroendocrinos (TPNE), necesario para determinar su grado de malignidad. Su estudio prequirúrgico comporta un impacto clínico significativo. La obtención del Ki-67 mediante USE-PAAF aún no está aclarada.
A safe technique using clips for covered self-expandable metal stent placement during endoscopic ultrasound-guided hepaticogastrostomy
Authors: A. Katanuma, H. Maguchi, K. Yane, T. Matsumori, A. Tomonari, T. Kin, K. Gon, K. Matsumoto, R. Takaki, M. Osanai, K. Takahashi
Journal: Endoscopy (2013)

Endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) is a new biliary drainage procedure, which uses a curved linear-array echoendoscope. Recently, the efficacy and safety of covered self-expandable metal stent (CSEMS) placement have been reported [1] [2] [3] [4] [5]. However, it is difficult to confirm correct release of the CSEMS endoscopically and stents can easily migrate into the abdominal cavity or intrahepatic bile duct. We report here on an innovation using clips that facilitates confirmation of the correct release point under fluoroscopic guidance.
Abstract 804: Class II transactivator (CIITA) transcription is reduced by the ER-E2 signaling pathway in breast cancer cells
Authors: Ahmed Mostafa, Dianne Codner, Christa Lewis, Kensuke Hirasawa, Sherri Christian, Viktor Steimle, Sheila Drover
Journal: Cancer Research (2011)

Abstract The immune response is regulated by the interaction of CD4+T-cells with human leukocyte antigen class II (HLA-II). HLA-II genes code for HLA-DR that display peptides from antigens, which have undergone proteolysis in the endocytic pathway. Peptide loading of HLA-DR molecules also requires the co-chaperones; Invariant chain (Ii) and HLA-DM. Regulation of HLA-II occurs mainly at the transcription level, through interaction of transcription factors with the class II transactivator (CIITA). We previously reported that discordant expression of HLA-II in breast cancer associated with decreased estrogen receptor (ER) levels and younger age at diagnosis, suggesting that the estrogen-ER signaling pathway is implicated in regulating HLA-II in breast carcinoma cells. Moreover, using an experimental model of ER+ and ER− breast cancer cell lines, we found that estradiol (E2) augmented expression in the ER− cell line but decreased expression in the ER+ transfectant. To address whether ER and E2 interfere with CIITA expression, we performed Western blotting and quantitative real-time PCR in the same experimental cell system. CIITA expression was markedly reduced in ER+, but not in ER− cells; furthermore, CIITA inversely correlated with ER levels. CIITA promoter activity was analysed using a luciferase reporter gene construct in cell lines, treated or not with E2 and/or ICI 182,780 (ER antagonist). CIITA luciferase activity was reduced in ER+ cells and was further inhibited by E2 and was restored by ICI. This suggests that ER may be recruited to the CIITA promoter and indirectly interfere with transcription of HLA-II genes. We further analyzed HLA-II in a wild type ER+ line, MCF-7, which was stimulated with IFN-γ or stably transfected with CIITA. HLA-DR surface expression, ascertained by flow cytometry, was upregulated in cells grown in E2-depleted medium. Western blotting confirmed that ER degradation by ICI in MCF-7 correlated with up regulation of CIITA and HLA-II. In aggregate, these results indicate that the E2-ER pathway interferes with CIITA and HLA-II expression. Investigations such as this should improve our understanding of how ER and estrogen modulate the antigen presentation capabilities and immune recognition of breast cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 804. doi:10.1158/1538-7445.AM2011-804
Abstract 3947: MicroRNA-127 (miR127) promotes cell migration and invasion in lung adenocarcinoma cells
Authors: Chiu-Yi Ho, Tai-Lin Lee, Hui-Chen Hsu, Jeremy Jian-Wei Chen, Meng-Feng Tsai
Journal: Cancer Research (2011)

Abstract MicroRNAs (miRNAs) are small non-coding RNAs, approximately 20-23 nucleotides in length. The specific functions of miRNAs involved in several cellular processes, including metabolism, development, differentiation, proliferation, apoptosis and tumorigenesis. In order to identify the invasion/metastasis related miRNA in lung cancer, the cell line model (lowly invasive lung cancer cells, CL1-0, and highly invasive lung cancer cells, CL1-5) and human miRNA microarray were carried out. In this study, we selected one of the invasion/metastasis related miRNAs, miR-127, derived from our microarray results for further functional characterization. Recently reports have indicated that human miR-127 was highly expressed in normal prostate and bladder tissues but was remarkably down-regulated in the corresponding tumors. miR-127 may function as a tumor suppressor in human bladder and prostate cancer cells. However, the role of the miR-127 in lung cancer cell proliferation, migration and invasion ability still remains unclear. The miR-127 expression level in various invasive capability lung cancer cell lines were also verified using stem-loop real time RT-PCR. We have constructed and established a miR-127 constitutive expression system in CL1-0 lung cancer cells using pSilencer3.1 vector, and we also knockdown the miR-127 expression in CL1-5 and A549 lung cancer cells using anti-miR127 modified oligonucleotide. Our results shown that over-expression of miR-127 can increase lung cancer cell proliferation, migration, and invasion ability. Finally, we suggest that miR-127 may be acting like an oncogene in lung adenocarcinoma cells. These results will provide important information and help us to understand the roles of miR127 in lung cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3947. doi:10.1158/1538-7445.AM2011-3947
FEVER INDUCED BRUGADA SYNDROME IS MORE COMMON THAN PREVIOUSLY SUSPECTED: A CROSSECTIONAL STUDY FROM AN ENDEMIC AREA
Authors: Pattara Rattanawong, Wasawat Vutthikraivit, Attawit Charoensri, Awapa Prombandankul, Napatt Kanjanahattakij, Tanawat Jongraksak, Treechada Wisaratapong, Tachapong Ngarmukos
Journal: Journal of the American College of Cardiology (2015)
P631 MESENCHYMAL STROMAL CELLS ENGINEERED TO PRODUCE IGF-I AMELIORATE LIVER FIBROSIS IN MICE
Authors: E.J. Fiore, J.M. Bayo Fina, M. García, M. Malvicini, R. Lloyd, F. Piccioni, M.M. Rizzo, E. Peixoto, C. Atorrasagasti, L. Alaniz, J.B. Aquino, G.D. Mazzolini
Journal: Journal of Hepatology (2014)
AB1027 Diagnostic Accuracy of Automated Determination of Antinuclear Antibodies (ANA) by Indirect REAction of Immunofluorescence on Human Hep-2 Cells (IIF-HEP-2) and Enzyme-Linked Immunosorbent Assay (ELISA) for Diagnosis of Systemic Lupus Erythematosus (SLE): Table 1.
Authors: Z.G. Verizhnikova, E.N. Aleksandrova, A.A. Novikov, T.A. Panafidina, N.V. Seredavkina, D. Roggenbuck, E.L. Nasonov
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> ANA – heterogeneous group of autoantibodies, reacting with the different components of nucleus and cytoplasm. ANA is main serological marker SLE and others systemic autoimmune rheumatic diseases (SARDs). The implementation of new highly productive methods of immune analysis with the use of automated systems into clinical practice creates the preconditions for standardization and improvement of reproducibility of ANA determination. <h3>Objectives</h3> To compare the diagnostic value of automated methods of ANA determination (IIF-HEp-2 and ELISA) in SLE. <h3>Methods</h3> We studied 83 patients (pts) with SLE (72 females, 9 males, median age 31,5, 16-65 years; the median diseases duration was 4 years, 2 months-36 years); 30 pts with others SARDs: 10 – Sjogren9s syndrome; 10 – systemic sclerosis; 10 – rheumatoid arthritis; 38 pts with other RD: 10 – psoriatic arthritis, 8 – gout, 10 – osteoarthrosis, 10 – ankylosing spondylitis; 30 healthy controls (HC). ANA was determinated with IIF-HEp-2 by the AKLIDES automated system ("Medipan GmbH", Germany) and ELISA on the "Alegria" analysator ("Orgentec", Germany). Positive results of ANA measuring corresponded with the values ≥1:160 (IIF-HEp-2) and ≥1,3 U (ELISA). <h3>Results</h3> The agreement of positive/negative results of ANA determination by IIF-HEp-2 and ELISA of RD pts and HC (n=181) was high (kappa=0,77). The results of ANA determination by the both methods matched in 88,4% of cases (positive – 47,5%, negative – 40,9% of pts). ANA determination by IIF-HEp-2 had more sensitivity in comparison with ELISA (93,9% and 81,9% respectively, p&lt;0,02). By the level of positive likelihood ratio (+LR) (&gt;2 and ≤5) both tests were applied to category of "useful" for SLE diagnosis. The negative likelihood ratio (-LR) of ANA determination by ELISA demonstrated "useful" diagnostic value of this test (&gt;0,2 and ≤0,5). The –LR ANA determination by IIF-HEp-2 (0,08, p&lt;0,001) was lower, than ELISA (0,25), and corresponded to the rank of tests, which are most "useful" for the exception of SLE diagnosis (&lt;0,2) (Table 1). <h3>Conclusions</h3> The results of the automated ANA determination by IIF-HEp-2 and ELISA have the high degree of correspondence and they allow to relate both tests to the amount of "useful" for SLE diagnosis. By the level sensitivity and –LR clinical value of ANA determination by IIF-HEp-2 had better results, then ELISA. <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.4028
S44 7 Days Actigraphy Monitoring is Equivalent to 14 Days Actigraphy Monitoring in Patients with Sleep Disorders
Authors: S Briscoe, E Hardy, C Kosky, A Williams, N Hart, J Steier
Journal: Thorax (2012)

<h3>Introduction</h3> Actigraphy is a valid measure to assess sleep and circadian rhythm abnormalities. It is listed in the diagnostic criteria for several sleep disorders where single night polysomnography is insufficient (ICSD-2). However, the optimal recording time for measurement remains unclear. We hypothesised that 7 days would provide similar data as recording for 14 days. <h3>Patients &amp; Methods</h3> We analysed 3 consecutive years of actigraphy data obtained within a tertiary sleep referral centre. Data were recorded continuously for two weeks using the AW2 Actiwatch (Cambridge NeuroTechnology, UK), using Actiwatch Sleep Analysis 5 software for analysis of the data. Parameters, including sleep efficiency (SE), sleep latency (SL), sleep fragmentation index (SFI), total sleep time (TST) and wake after sleep onset (WASO), were classified into week 1, week 2 and an overall average for the duration of 14 days. In addition, two experienced consultants working in the sleep laboratory compared the results of week 1 vs. week 2 independently, visually analysing the data for circadian rhythmicity and fragmentation of the pattern; allowing calculation of the itraclass correlation coefficient (ICC), κ. <h3>Results</h3> The actigraphies of 239 patients (51.9% (124) male; mean age 42 (16) years) were analysed. There was no difference in SE, SL, SFI or WASO between week 1, week 2 and 14 days average recording. A difference was found between TST week 1 (399.9, 95% CI 389.9–409.9 mins) and TST week 2 (388.7, 95% CI 378.3–399.1 mins), but not between TST for 14 days average recording and either week (394.3, 95% CI 384.7–403.9 mins). Independent scorers achieved a strong agreement in the rhythmicity of the sleep pattern (ICC κ 0.734, p&lt;0.001) and a low agreement for the fragmentation of the pattern (ICC κ 0.380, p&lt;0.001). <h3>Conclusion</h3> One week of actigraphy provides similar data to two week actigraphy, despite subtle differences between different weeks. One week actigraphy should be recommended as standard to maximise efficiency of the clinical service.
Abstract 272: Transcriptional regulation of REG4, a novel diagnostic marker of gastrointestinal cancer, by CDX2
Authors: Yutaka Naito, Naohide Oue, Kazuhiro Sentani, Naoya Sakamoto, Katsuhiro Anami, Tetsutaro Hayashi, Naohiro Uraoka, Htoo Zarni Oo, Yuta Wakamatsu, Wataru Yasui
Journal: Cancer Research (2011)

Abstract Regenerating gene family, member 4 (REG4, encodes Reg IV), was originally identified from cDNA library of inflammatory bowel disease tissues, and is specifically expressed in gastrointestinal tract. We have identified REG4 as an overexpressed gene in gastric cancer from SAGE analysis in gastric cancer tissues and noticed as a novel serum marker of cancer. The expression of Reg IV is associated with intestinal mucin differentiation, and RegIV is expressed in CDX2-positive cells. CDX2, the intestine-specific caudal-related homeobox transcription factor, plays a key role in intestinal development and differentiation. In the present study, we investigated whether CDX2 induce RegIV expression. Western blot analysis indicated that forced expression of CDX2 in HT29 colon cancer cell line induced Reg IV expression. In silico analysis of 2-kilobase upstream region of REG4 revealed at least four putative CDX2 consensus sequences. To assess the role of these putative CDX2 consensus sequences in regulating REG4 transcription, we performed luciferase assay into HSC39 gastric cancer cell line, in which CDX2 is highly expressed. Reporter gene constructs containing 2-kilobase of 5’-flanking region from the REG4 gene showed strong reporter activity. Significant change of reporter activity was not observed between 2.0 and 0.65-kilobase upstream region of REG4 constructs, apart from that significant lower expression level at 0.1-kilobase. These results suggest that expression of REG4 is regulated by CDX2, and upstream region of REG4 has enhancer sequences at proximal region at 0.1-kilobase upstream region. Based on the analysis of single mutation in the putative CDX2 consensus sequences in the 5’-flanking region of the REG4 gene, two putative CDX2 consensus sequences were found to activate REG4 expressions. Therefore, RegIV may be a key factor mediating CDX2 function in intestinal determination. To identify the CDX2-binding site in detail, ChIP assay and mutagenesis with multiple mutations in the putative CDX2 consensus sequences must be carried out. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 272. doi:10.1158/1538-7445.AM2011-272
Abstract 1514: Oncolytic viral therapy for glioblastoma induces robust natural killer cell activation
Authors: Christopher A. Alvarez-Breckenridge, Jianhua Yu, Jason Pradarelli, Tom Liu, Balveen Kaur, Michael Caligiuri, E. Antonio Chiocca
Journal: Cancer Research (2010)

Abstract Oncolytic viral (OV) therapy is a promising treatment being tested for safety and efficacy. Natural killer cells (NK) limit viral infections, and previous work suggests they may similarly attenuate virotherapy. We used flow cytometry to evaluate the temporal pattern of NK recruitment in intracranial tumors (U87DEGFR glioma) treated with the oncolytic herpes virus, rQnestin 34.5, or PBS. Quantification of NK recruitment into intracranial tumors treated with rQnestin34.5 or PBS revealed significant enrichment of these cells into the tumor hemisphere at early timepoints and continuing through 72 hours (2.2-fold, p=0.02) following infection. Moreover, NK cells recruited to the site of inoculation exhibited an activated phenotype, including enhanced CD69, CD62L, CD27, NKG2D, and CD127 staining compared to PBS treated mice. This robust NK response was demonstrated to be detrimental to OV efficacy through the enhanced survival of NK depleted mice treated with OV compared to OV treated mice containing NK cells (median survival 31.6 days compared to median survival 23.6 days, p=0.04). Since NK cells are recruited into the tumor microenvironment following infection, we postulated that OV infection of glioma cells was also activating NK cells. We measured granzyme B and IFNg production by NK cells in the presence of infected or uninfected glioma. Our results indicate that glioma mediated activation of NK cells was augmented by OV infection. Additionally, these findings were corroborated with increased NK killing of OV infected glioma compared to uninfected glioma. Due to the enhanced NK cytotoxic properties induced by OV infection, we proceeded to investigate the expression of NK activating ligands on the surface of infected glioma cells that could potentially mediate this effect. Focusing on ligands for NKG2D and the natural cytotoxicity receptors (NKp30, NKp44, and NKp46), viral infection of cultured gliomas was shown to upregulate ligand expression for NKG2D (24%, p=0.004), NKp46 (41%, p=0.01), and NKp30 (2.75-fold, p=0.001) while selectively downregulating ligand expression for NKp44 (2.8-fold, p=0.005). These findings suggest that viral infection of gliomas is altering the ligand signature for NK activation. Since NK cells possess both antiviral and antitumor properties, further work will investigate if virus induced NK activating signals can be selectively blocked in order to enhance viral efficacy while maintaining NK antitumor efficacy. This is the first study investigating the changes in NK cell activation and recruitment in gliomas upon oncolytic HSV-1 treatment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1514.
Abstract P1-13-09: Marked Inhibition of Both Invasion and Proliferation by Zoledronic Acid and the Epidermal Growth Factor Receptor Inhibitor Gefitinib in a Bone-Seeking Clone of Breast Cancer
Authors: M Oda, K Iwaya, K Yamada, H Kaise, A Ogata, F Kimura, T Kobayashi, O Matsubara, T Yoneda, N. Kouno
Journal: Cancer Research (2010)

Abstract Background: Zoledronic acid (Zol) is the most potent inhibitor of bone resorption in bisphosphonates. In order to examine whether the inhibitory effect is involved in the mechanism of bone metastasis in breast cancer, a bone-seeking clone was examined. It was established by repeated sequential passages of metastatic cells from bone in nude mice and in vitro, and it exclusively metastasizes to bone with larger osteolytic lesions than MDA-MB-231 parental cells. Recent findings indicate that epidermal growth factor (EGF) signaling is also an important mediator of bone metastasis. We examined the synergistic effects of gefitinib on the proliferation and invasion of a bone-seeking clone compared with MDA-MB-231. Materials and Methods: The cellular proliferation was estimated using WST-1. Migration in the matrigel in a Boyden chamber was applied to the invasion assay. Results: Treatment with 1 µM of Zol reduced 51% of cell proliferation in the bone-seeking clone; however, the inhibited rate was 37% in MDA-MB-231. The migration was also inhibited with 10 µM of Zol (bone-seeking clone: 69% reduction, MDA-MB-231: 54% reduction). The reduction rates of both assays by Zol treatment were significantly higher in the bone-seeking clone than in MDA-MB-231. Treatment with 10 µM of Zol plus 1 µM of gefitinib resulted in a proliferation reduction rate of 27% compared to that with 10 µM Zol treatment in the bone-seeking clone and no reduction in MDA-MB-231. The migration reduction rate was 88% in the bone-seeking clone and no reduction in MDA-MB-231. There was a significant difference between the bone-seeking clone and MDA-MB-231 in the reduction rates of both cellular proliferation and migration. Although the effect of gefitinib was synergistic with that of Zol in the bone-seeking clone, it was not clear in MDA-MB-231. Conclusion: There appear to be at least two independent signaling pathways that occur in osteolytic bone metastasis. One is involved in the bone resorption of bisphosphonates, and the other is an EGFR signaling pathway. Clinically, the combination of Zol and gefitinib is suggested to be a strong therapy specific to bone metastasis. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P1-13-09.