tbuckley/cabot-search huggingface dataset:
Expression of Ly 1, Ly 2, Thy 1, and TL differentiation antigens on mouse T-cell tumors.
Authors: B J Mathieson, P S Campbell, M Potter, R Asofsky
Journal: The Journal of Experimental Medicine (1978)

Transplanted lymphomas, most of thymic origin, induced in BALB/c mice with 1-ethyl-1-nitrosourea (ENU) and transplanted spontaneously occurring lymphomas of AKR mice were examined for the expression of the T-cell antigens Ly, TL, and Thy 1 by using three serological methods. Most (11 of 13) of the Thy 1+ and/or TL+ tumors, i.e., T-cell tumors, expressed high levels of either Ly 1 or Ly 2 antigen, but not both. Thus most thymic lymphocytic tumors expressed restricted Ly phenotypes comparable to phenotypes previously described for functional peripheral T cells. Because tumor phenotypes were stable over a number of transplant generations, they therefore appeared to be an intrinsic property of the specific tumors. The majority of the BALB/c lymphomas were Ly 1- 2+ and also positive with anti-TL antiserum. This predominant phenotype on the BALB/c tumors may be related to either the mode of tumor induction or to the mouse strain, but since the restricted Ly pattern was observed both in BALB/c and AKR tumors, the phenotypic restriction itself is not a consequence of either of these factors. Tumor induction by ENU per se is not responsible for Ly or TL ,ntigen expression since several non-T-cell BALB/ c tumors, also induced by ENU, did not express either Ly or TL antigens. Data presented here suggest that the target cell for leukemogenesis may be a partially differentiated thymus cell. The restricted expression of Ly antigens on differentiating thymus cells to either the (formula: see text), phenotype may occur before the loss of TL antigen.
High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research
Authors: Kathrin Reichwald, Chris Lauber, Indrajit Nanda, Jeanette Kirschner, Nils Hartmann, Susanne Schories, Ulrike Gausmann, Stefan Taudien, Markus B Schilhabel, Karol Szafranski, Gernot Glöckner, Michael Schmid, Alessandro Cellerino, Manfred Schartl, Christoph Englert, Matthias Platzer
Journal: Genome biology (2009)

The annual fish Nothobranchius furzeri is the vertebrate with the shortest known life span in captivity. Fish of the GRZ strain live only three to four months under optimal laboratory conditions, show explosive growth, early sexual maturation and age-dependent physiological and behavioral decline, and express aging related biomarkers. Treatment with resveratrol and low temperature significantly extends the maximum life span. These features make N. furzeri a promising new vertebrate model for age research. To contribute to establishing N. furzeri as a new model organism, we provide a first insight into its genome and a comparison to medaka, stickleback, tetraodon and zebrafish. The N. furzeri genome contains 19 chromosomes (2n = 38). Its genome of between 1.6 and 1.9 Gb is the largest among the analyzed fish species and has, at 45%, the highest repeat content. Remarkably, tandem repeats comprise 21%, which is 4-12 times more than in the other four fish species. In addition, G+C-rich tandem repeats preferentially localize to centromeric regions. Phylogenetic analysis based on coding sequences identifies medaka as the closest relative. Genotyping of an initial set of 27 markers and multi-locus fingerprinting of one microsatellite provides the first molecular evidence that the GRZ strain is highly inbred. Our work presents a first basis for systematic genomic and genetic analyses aimed at understanding the mechanisms of life span determination in N. furzeri.
Increased Frequency of CD4+ Cells Expressing CD161 in Cancer Patients
Authors: Eleni G. Iliopoulou, Michalis V. Karamouzis, Ioannis Missitzis, Alexandros Ardavanis, Nectaria N. Sotiriadou, Constantin N. Baxevanis, Gerasimos Rigatos, Michael Papamichail, Sonia A. Perez
Journal: Clinical Cancer Research (2006)

Abstract Purpose: Although the function of natural killer receptors on T cells infiltrating tumors and their potential effect on antitumor immunity has been investigated, little is known about T cells expressing NKR-P1A (CD161) in cancer patients. In the present study, we examined T cells expressing CD161 in the peripheral blood, the tumor tissue and in malignant effusions of patients with several types of malignancies. Experimental Design: Expression of CD161 in CD4+ or CD8+ (lacking CD56) T cells isolated from peripheral blood (n = 61), tumor specimens (n = 8), and malignant effusions (n = 37) of cancer patients was examined using four-color flow cytometry. Proliferative capacity and cytokine production of purified CD4+CD161+CD56− cells were studied after weak or strong stimulation, with or without costimulation, in the presence or absence of interleukin 2. The possible regulatory function of activated CD4+CD161+CD56− cells on T-cell alloresponses was also investigated. Results: CD4+ cells expressing CD161 were increased in cancer patients, compared with healthy individuals. This increase in the peripheral blood of cancer patients positively correlated with disease stage and was augmented at the tumor site. Phenotypic analysis revealed that CD4+CD161+ cells are memory T cells, with low expression of activation markers. CD4+CD161+ cells play an immunoregulatory role through cytokine production, because upon receiving costimulatory signals via CD28, they exert suppressive activity on autologous peripheral blood mononuclear cell alloresponses. Conclusions: CD4+CD161+CD56− cells represent a distinct memory T-cell population significantly increased in cancer patients. Depending on the type of signals provided by the tumor microenvironment, CD4+CD161+ cells may regulate the immune response.
Alternating Systemic and Hepatic Artery Infusion Therapy for Resected Liver Metastases From Colorectal Cancer: A North Central Cancer Treatment Group (NCCTG)/ National Surgical Adjuvant Breast and Bowel Project (NSABP) Phase II Intergroup Trial, N9945/CI-66
Authors: Steven R. Alberts, Mark S. Roh, Michelle R. Mahoney, Michael J. O'Connell, David M. Nagorney, Lawrence Wagman, Thomas C. Smyrk, Timothy L. Weiland, Lily Lau Lai, Roderich E. Schwarz, Roy Molina, Todor Dentchev, John S. Bolton
Journal: Journal of Clinical Oncology (2010)

PURPOSE Prior trials have shown that surgery followed by hepatic artery infusion (HAI) of floxuridine (FUDR) alternating with systemic fluorouracil improves survival rates. Oxaliplatin combined with capecitabine has demonstrated activity in advanced colorectal cancer. Based on this observation a trial was conducted to assess the potential benefit of systemic oxaliplatin and capecitabine alternating with HAI of FUDR. The primary end point was 2-year survival. PATIENTS AND METHODS Patients with liver-only metastases from colorectal cancer amenable to resection or cryoablation were eligible. HAI and systemic therapy was initiated after metastasectomy. Alternating courses of HAI consisted of 0.2 mg/m(2)/d FUDR and dexamethasone, day 1 through 14 weeks 1 and 2. Systemic therapy included oxaliplatin 130 mg/m(2) day 1 with capecitabine at 1,000 mg/m(2) twice daily, days 1 through 14, weeks 4 and 5. Two additional 3-week courses of systemic therapy were given. Capecitabine was reduced to 850 mg/m(2) twice daily after interim review of toxicity. Results Fifty-five of 76 eligible patients were able to initiate protocol-directed therapy and completed median of six cycles (range, one to six). Three postoperative or treatment-related deaths were reported. Overall, 88% of evaluable patients were alive at 2 years. With a median follow-up of 4.8 years, a total of 30 patients have had disease recurrence, 11 involving the liver. Median disease-free survival was 32.7 months. CONCLUSION Alternating HAI of FUDR and systemic capecitabine and oxaliplatin met the prespecified end point of higher than 85% survival at 2 years and was clinically tolerable. However, the merits of this approach need to be established with a phase III trial.
SPECIFIC EXERCISE TARGETING THE SEMITENDINOSUS IN FEMALE ACL-RECONSTRUCTED ATHLETES
Authors: HB Lauridsen, J Bencke, K Thorborg, LL Andersen, S Sonne-Holm, P Aagaard, P Hölmich, MK Zebis
Journal: British Journal of Sports Medicine (2014)

Background The most commonly used graft in ACL-reconstructions is harvested from the semitendinosus muscle (ST) which causes atrophy and reduced muscle strength in the hamstrings. As the ST serves as an important ACL agonist, exercises that causes high levels of ST muscle activity after ACL-reconstruction, is of great interest to prevent re-rupture of the ACL. Objectives The purpose of the present study was to examine the level of neuromuscular hamstring muscle activity during two commonly used strength exercises, the two hand Kettlebell Swing (KS) and the Nordic Hamstring (NH), in previously ACL-reconstructed female athletes. Design Cross-sectional study. Settings Biomechanical laboratory. Participants Recruitment ongoing – the study is planned to include 20 participants. Preliminary data from five female athletes (age 28±7 yrs) with a total of 6 ACL-reconstructed knees are presented. In all cases, ST tendon was used as graft. The time between surgery and testing was 52±47months. Intervention Ten consecutive KS's were performed corresponding to a maximal load (12–16 kg) where correct technique was maintained. Additional, five consecutive NH curls were executed. Muscle activity for the hamstring muscles was obtained during the exercise evaluation and during hamstring MVC. Main outcome measurements Peak EMG of the medial (ST) and lateral (BF) hamstring during KS and NH was normalized to peak EMG amplitude during MVC. Results The exercise evaluation showed a significantly higher peak ST EMG activity during KS than NH (91±23% vs. 63±23%, respectively), P=.011. No difference was observed in peak BF EMG activity between exercises (82±30% vs. 66±36%, respectively), P=.125. Conclusion The present finding suggests that the KB exercise could be introduced in rehabilitation when aiming at enhanced ability to recruit ST during forceful movements. However, to avoid adverse effects in the early phase of rehabilitation, caution must be taken when introducing high-intensity training among ACL reconstructed patients.
Stroke Prevention in Nonvalvular Atrial Fibrillation
Authors: G W Albers, J E Atwood, J Hirsh, D G Sherman, R A Hughes, S J Connolly
Journal: Annals of Internal Medicine (1991)

▪ There has been considerable uncertainty about the best way to prevent stroke in patients with nonvalvular atrial fibrillation. Recent studies have suggested that low-dose warfarin therapy, in addition to producing fewer bleeding complications, may be as effective as higher-dose therapy in preventing thromboembolic events. Four large, prospective, randomized trials have examined the risks and benefits of warfarin therapy for stroke prophylaxis in patients with nonvalvular atrial fibrillation. All four studies showed a substantially reduced incidence of stroke and a low incidence of significant bleeding in patients treated with warfarin. One of these studies also showed that aspirin reduced the incidence of stroke. The benefits associated with long-term low-dose warfarin therapy appear to exceed the risks for serious bleeding in most patients with atrial fibrillation. Aspirin may be a viable therapeutic option for patients who are unable to take warfarin or for those in subgroups at a low risk for stroke.
Effect of antioxidants on the occurrence of pre-eclampsia in women at increased risk: a randomised trial
Authors: Lucy C Chappell, Paul T Seed, Annette L Briley, Frank J Kelly, Rosalind Lee, Beverley J Hunt, Kiran Parmar, Susan J Bewley, Andrew H Shennan, Philip J Steer, Lucilla Poston
Journal: The Lancet (1999)
miR-17 overexpression in cystic fibrosis airway epithelial cells decreases interleukin-8 production
Authors: Irene K. Oglesby, Sebastian F. Vencken, Raman Agrawal, Kevin Gaughan, Kevin Molloy, Gerard Higgins, Paul McNally, Noel G. McElvaney, Marcus A. Mall, Catherine M. Greene
Journal: European Respiratory Journal (2015)

Interleukin (IL)-8 levels are higher than normal in cystic fibrosis (CF) airways, causing neutrophil infiltration and non-resolving inflammation. Overexpression of microRNAs that target IL-8 expression in airway epithelial cells may represent a therapeutic strategy for cystic fibrosis. IL-8 protein and mRNA were measured in cystic fibrosis and non-cystic fibrosis bronchoalveolar lavage fluid and bronchial brushings (n=20 per group). miRNAs decreased in the cystic fibrosis lung and predicted to target IL-8 mRNA were quantified in βENaC-transgenic, cystic fibrosis transmembrane conductance regulator ( Cftr ) -/- and wild-type mice, primary cystic fibrosis and non-cystic fibrosis bronchial epithelial cells and a range of cystic fibrosis versus non-cystic fibrosis airway epithelial cell lines or cells stimulated with lipopolysaccharide, Pseudomonas -conditioned medium or cystic fibrosis bronchoalveolar lavage fluid. The effect of miRNA overexpression on IL-8 protein production was measured. miR-17 regulates IL-8 and its expression was decreased in adult cystic fibrosis bronchial brushings, βENaC-transgenic mice and bronchial epithelial cells chronically stimulated with Pseudomonas -conditioned medium. Overexpression of miR-17 inhibited basal and agonist-induced IL-8 protein production in F508del-CFTR homozygous CFTE29o − tracheal, CFBE41o − and/or IB3 bronchial epithelial cells. These results implicate defective CFTR, inflammation, neutrophilia and mucus overproduction in regulation of miR-17. Modulating miR-17 expression in cystic fibrosis bronchial epithelial cells may be a novel anti-inflammatory strategy for cystic fibrosis and other chronic inflammatory airway diseases.
Atypical symmetrical lipomatosis with lipoatrophy of the face (mimicking HAART-related lipodystrophy)
Authors: I. Castiñeiras Mato, M. Almagro Sánchez, T. Chouciño, J. del Pozo Losada, B. Fernández Jorge, E. Fonseca Capdevila
Journal: British Journal of Dermatology (2007)
Relation of Donor Age and Preexisting Coronary Artery Disease on Angiography and Intracoronary Ultrasound to Later Development of Accelerated Allograft Coronary Artery Disease
Authors: Shao-Zhou Gao, Sharon A Hunt, Edwin L Alderman, David Liang, Alan C Yeung, John S Schroeder
Journal: Journal of the American College of Cardiology (1997)

Objectives. This study assessed the influence of donor age and preexisting donor coronary artery disease on the later development of allograft coronary artery disease, ischemic events and overall survival. Background. The increasing demand for heart donors has led to a tendency to liberalize age criteria for donor acceptability. Methods. A total of 233 consecutive heart transplant recipients who had baseline, early postoperative and follow-up coronary angiograms, as well as a subset of 47 patients with baseline intracoronary ultrasound imaging recordings, were analyzed (mean 3.8 years of follow-up). Patients were subclassified according to the presence of donor coronary artery disease on the baseline angiogram and stratified at age 40 years. Results. Patients without evidence of preexisting coronary artery disease on a baseline angiogram (n = 219) were significantly less likely to develop new disease than the 14 patients with preexisting coronary artery disease (p = 0.002). Although older donors exhibited earlier coronary artery disease than younger donors at 3 years of follow-up, there was no difference by 5 years (p = 0.25). There was no difference in survival or probability of developing ischemic events between the groups. Baseline ultrasound imaging revealed substantial disease in 7 of 9 older donated hearts, and in only 7 of 38 younger donated hearts (p = 0.002). Preexisting coronary artery disease, nonuse of calcium channel blocking agents, older donor age, posttransplantation cytomegalovirus infection, elevated very low density lipoprotein levels and previous ischemic heart disease in the recipient were significant predictors of allograft coronary artery disease. Conclusions. Heart donors with angiographic evidence of pre-existing coronary artery disease and older donors are more likely to develop new allograft coronary artery disease by 3 years. However, there is no difference in patient survival or freedom from ischemic events between younger and older donors at a mean follow-up of 3.8 years. (J Am Coll Cardiol 1997;29:623–9)
Inhaled Nitric Oxide Improves Outcomes After Successful Cardiopulmonary Resuscitation in Mice
Authors: Shizuka Minamishima, Kotaro Kida, Kentaro Tokuda, Huifang Wang, Patrick Y. Sips, Shizuko Kosugi, Joseph B. Mandeville, Emmanuel S. Buys, Peter Brouckaert, Philip K. Liu, Christina H. Liu, Kenneth D. Bloch, Fumito Ichinose
Journal: Circulation (2011)

Background— Sudden cardiac arrest (CA) is a leading cause of death worldwide. Breathing nitric oxide (NO) reduces ischemia/reperfusion injury in animal models and in patients. The objective of this study was to learn whether inhaled NO improves outcomes after CA and cardiopulmonary resuscitation (CPR). Methods and Results— Adult male mice were subjected to potassium-induced CA for 7.5 minutes whereupon CPR was performed with chest compression and mechanical ventilation. One hour after CPR, mice were extubated and breathed air alone or air supplemented with 40 ppm NO for 23 hours. Mice that were subjected to CA/CPR and breathed air exhibited a poor 10-day survival rate (4 of 13), depressed neurological and left ventricular function, and increased caspase-3 activation and inflammatory cytokine induction in the brain. Magnetic resonance imaging revealed brain regions with marked water diffusion abnormality 24 hours after CA/CPR in mice that breathed air. Breathing air supplemented with NO for 23 hours starting 1 hour after CPR attenuated neurological and left ventricular dysfunction 4 days after CA/CPR and markedly improved 10-day survival rate (11 of 13; P =0.003 versus mice breathing air). The protective effects of inhaled NO on the outcome after CA/CPR were associated with reduced water diffusion abnormality, caspase-3 activation, and cytokine induction in the brain and increased serum nitrate/nitrite levels. Deficiency of the α1 subunit of soluble guanylate cyclase, a primary target of NO, abrogated the ability of inhaled NO to improve outcomes after CA/CPR. Conclusions— These results suggest that NO inhalation after CA and successful CPR improves outcome via soluble guanylate cyclase–dependent mechanisms.
Recent advances in selective opioid receptor agonists and antagonists
Authors: Masakatsu Eguchi
Journal: Medicinal Research Reviews (2003)

Abstract Opioid analgesics provide outstanding benefits for relief of severe pain. The mechanisms of the analgesia accompanied with some side effects have been investigated by many scientists to shed light on the complex biological processes at the molecular level. New opioid drugs and therapies with more desirable properties can be developed on the bases of accurate insight of the opioid ligand–receptor interaction and clear knowledge of the pharmacological behavior of opioid receptors and the associated proteins. Toward this goal, recent advances in selective opioid receptor agonists and antagonists including opioid ligand–receptor interactions are summarized in this review article. © 2003 Wiley Periodicals, Inc. Med Res Rev, 24, No. 2, 182–212, 2004
Computerised neuropsychological testing
Authors: A Collie, P Maruff
Journal: British Journal of Sports Medicine (2003)

Should it be performed by neuropsychologists or team doctors?

The dual roles of neuropsychological testing in sports concussion are well established. Neuropsychological assessment may aid understanding of the brain structures and processes underlying concussion and the Post Concussion Syndrome. Although this is a primary goal of neuropsychologists working with concussed athletes, a more immediate role lies in facilitating effective post concussion medical management of individual athletes.1 Over the past two decades, “paper and pencil” neuropsychological tests have been used to aid concussion management in many sports.2–4 However, use of such tests has generally been limited to athletes performing at the elite level. The wide scale use of paper and pencil tests in sports medicine is limited by the requirement that test administration and interpretation be undertaken by trained personnel—for example, neuropsychologists, and that administration is usually on a one-to-one basis. This makes neuropsychological assessment of entire sporting teams time consuming, expensive, and beyond the means of most junior and amateur contact sporting organisations.

These practical limitations, combined with the psychometric limitations of some paper and pencil tests,5 have led to the development of a number of computerised neuropsychological test batteries.6,7 These computerised …
Symptoms Simulating those of Angina Pectoris, Arising under the Local Application of Ergotin
Authors: T. N. Mills
Journal: BMJ (1882)

Lying in the prone position, or resorting to the genu-pectoral posi- tion from time to time, will also assist in encouraging a return of the uterus to its normal position, where it is retroverted; though at first the patient will probably experience much inconvenience, and perhaps state that it is impossible for her to carry out the suggestion.The importance of so doing must be explained to her, and she must be en- couraged to persevere in her attempts, gradually prolonging the time as she finds it practicable.The patient must avoid long standing or
Rhodamine-RCA in vivo labeling guided laser capture microdissection of cancer functional angiogenic vessels in a murine squamous cell carcinoma mouse model.
Authors: Finie Hunter, Jianwu Xie, Cameron Trimble, Monica Bur, KingCP Li
Journal: Molecular Cancer (2006)

Cancer growth, invasion and metastasis are highly related to tumor-associated neovasculature. The presence and progression of endothelial cells in cancer is chaotic, unorganized, and angiogenic vessels are less functional. Therefore, not all markers appearing on the chaotic endothelial cells are accessible if a drug is given through the vascular route. Identifying endothelial cell markers from functional cancer angiogenic vessels will indicate the accessibility and potential efficacy of vascular targeted therapies. In order to quickly and effectively identify endothelial cell markers on the functional and accessible tumor vessels, we developed a novel technique by which tumor angiogenic vessels are labeled in vivo followed by Laser Capture Microdissection of microscopically isolated endothelial cells for genomic screening. Female C3H mice (N = 5) with established SCCVII tumors were treated with Rhodamine-RCA lectin by tail vein injection, and after fluorescence microscopy showed a successful vasculature staining, LCM was then performed on frozen section tissue using the PixCell II instrument with CapSure HS caps under the Rhodamine filter. By this approach, the fluorescent angiogenic endothelial cells were successfully picked up. As a result, the total RNA concentration increased from an average of 33.4 ng/ul +/- 24.3 (mean +/- S.D.) to 1913.4 ng/ul +/- 164. Relatively pure RNA was retrieved from both endothelial and epithelial cells as indicated by the 260/280 ratios (range 2.22–2.47). RT-PCR and gene electrophoresis successfully detected CD31 and Beta-Actin molecules with minimal Keratin 19 expression, which served as the negative control. Our present study demonstrates that in vivo Rhodamine RCA angiogenic vessel labeling provided a practical approach to effectively guide functional endothelial cell isolation by laser capture microdissection with fluorescent microscopy, resulting in high quality RNA and pure samples of endothelial cells pooled for detecting genomic expression.