tbuckley/cabot-search huggingface dataset:
Annexin A8 Is Up-Regulated During Mouse Mammary Gland Involution and Predicts Poor Survival in Breast Cancer
Authors: Torsten Stein, Karen N. Price, Joanna S. Morris, Victoria J. Heath, Roderick K. Ferrier, Alexandra K. Bell, Marie-Anne Pringle, René Villadsen, Ole W. Petersen, Guido Sauter, Gareth Bryson, Elizabeth A. Mallon, Barry A. Gusterson
Journal: Clinical Cancer Research (2005)

Abstract Purpose: Microarray studies have linked Annexin A8 RNA expression to a “basal cell–like” subset of breast cancers, including BRCA1-related cancers, that are characterized by cytokeratin 5 (CK5) and CK17 expression and show poor prognosis. We assessed Annexin A8's contribution to the overall prognosis and its expression in normal, benign, and cancerous tissue and addressed Annexin A8's physiologic role in the mammary gland. Experimental Design: Using microarrays and reverse transcription-PCR, the Annexin A8 expression was studied during mouse mammary gland development and in isolated mammary structures. Reverse transcription-PCR on cultured human luminal and basal cells, along with immunocytochemistry on normal and benign breast tissues, was used for cellular localization. Annexin A8's prognostic relevance and its coexpression with CK5 were assessed on tissue arrays of 1,631 cases of invasive breast cancer. Coexpression was further evaluated on a small cohort of 14 BRCA1-related breast cancers. Results: Annexin A8 was up-regulated during mouse mammary gland involution and in pubertal ductal epithelium. Annexin A8 showed preferred expression in cultured basal cells but predominant luminal expression in normal human breast tissue in vivo. Hyperplasias and in situ carcinomas showed a strong staining of basal cells. Annexin A8 expression was significantly associated with grade (P &amp;lt; 0.0001), CK5 (P &amp;lt; 0.0001), and estrogen receptor status (P &amp;lt; 0.0001); 85.7% BRCA1-related breast tumors coexpressed Annexin A8 and CK5. Conclusion: Annexin A8 is involved in mouse mammary gland involution. In humans, it is a luminally expressed protein with basal expression in cell culture and in hyperplasia/ductal carcinoma in situ. Expression in invasive breast carcinomas has a significant effect on survival (P = 0.03) but is not independent of grade or CK5.
Education and Mortality in Type 2 Diabetes
Authors: Ronald P. Wilder
Journal: Diabetes Care (2003)

Results from a population-based survey Our objective was to evaluate the diagnostic proficiency of the World Health Organization (WHO) and the National Cholesterol Education Program (NCEP)-III definitions (1,2) for the metabolic syndrome in a Mexican nationwide, population-based survey.Details of the sampling procedures have been previously described (3).The population was composed of 2,158 men and women aged 20 -69 years sampled after a 9-to 12-h fasting period.For the WHO criteria, insulin resistance was diagnosed if a nondiabetic case had fasting insulin concentrations Ն126 pmol/l (21 U/ml) (Ͼ75th percentile in Mexican adults).The ageadjusted prevalence was 13.61% for the WHO criteria (n ϭ 268) and 26.6% for the NCEP-III definition (n ϭ 574).After excluding patients with diabetes, the prevalence was 9.2 and 21.4%, respectively.The agreement between the definitions was assessed in 1,969 subjects; 189 cases were eliminated due to the lack of a urine sample.The number of abnormal cases was lower using the WHO criteria.Only 237 of the 545 subjects (43.4%) who fulfilled the NCEP criteria were diagnosed as affected using the WHO definition.Just 16 of 253 cases (6.3%) detected by the WHO definition did not fulfill the NCEP definition.The agreement between the criteria was moderate ( ϭ 0.507).On the other hand, the subjects diagnosed using the WHO recommendations had a worse profile than the cases detected by the NCEP-III definition only-they had a higher BMI and higher non-HDL cholesterol, triglyceride, and glucose concentrations.The demonstration of insulin resistance among the nondiabetic population caused the lack of agreement in 202 of the 242 cases that fulfilled the NCEP definition but failed the WHO criteria.Other reasons for disparity were the higher thresholds used by the WHO criteria; these differences explained the lack of agreement in 66 of the 152 cases with diabetes.In conclusion, the prevalence of the metabolic syndrome is influenced by the selection of the diagnostic criteria.The WHO criteria identified a lower number of cases than the NCEP-III definition.These differences were explained mainly by the inclusion of abnormally high insulin concentrations as a diagnostic criterion.However, the presence of insulin resistance may help to identify patients more severely affected (4).
Improvement of Left Ventricular Remodeling and Function by Hydroxymethylglutaryl Coenzyme A Reductase Inhibition With Cerivastatin in Rats With Heart Failure After Myocardial Infarction
Authors: Johann Bauersachs, Paolo Galuppo, Daniela Fraccarollo, Michael Christ, Georg Ertl
Journal: Circulation (2001)

Background Hydroxymethylglutaryl coenzyme A reductase inhibitors (statins) attenuate angiotensin II-induced cellular signaling. Because angiotensin II is involved in left ventricular (LV) remodeling after myocardial infarction (MI), we examined the effects of statin treatment in an experimental model of chronic heart failure after MI. Methods and Results Rats with extensive MI were treated with placebo or cerivastatin (0.3 mg/kg per day) as a dietary supplement or via gavage for 11 weeks starting on the 7th postoperative day. Infarct size and cholesterol levels were similar among all groups. LV cavity area, an index of LV dilatation, was reduced in MI rats on cerivastatin compared with placebo. LV end-diastolic pressure was increased in MI rats on placebo (24.1±4.1 mm Hg versus sham: 5.1±0.3 mm Hg; P &lt;0.01), and it was significantly reduced by cerivastatin treatment (13.7±2.7 mm Hg; P &lt;0.05 versus placebo). Cerivastatin partially normalized LV dP/dt max and dP/dt min , indices of LV systolic and diastolic function, which were significantly reduced in MI rats on placebo. Improvement of LV function by cerivastatin was accompanied by a reduced expression of collagen type I and β-myosin heavy chain. LV endothelial nitric oxide synthase was increased, whereas the nitrotyrosine protein level was decreased in MI rats by cerivastatin treatment. Conclusions Cerivastatin improved LV remodeling and function in rats with heart failure. This effect was associated with an attenuated LV expression of fetal myosin heavy chain isoenzymes and collagen I. Statin treatment may retard the progression of chronic heart failure.
IgE‐mediated allergy to phytase – a new animal feed additive
Authors: X. Baur, S. Melching‐Kollmuss, F. Koops, K. Straßburger, A. Zober
Journal: Allergy (2002)

Background: Although fungal phytase is frequently used as an additive to animal feed few investigations of its allergenic property have been conducted. Methods: Fifty‐three subjects occupationally exposed to powdered phytase from Aspergillus niger were studied. Exposure data and symptoms were registered by the company physician. Results: Thirty‐eight subjects complained of work‐related respiratory symptoms and 14 of them showed phytase‐specific IgE antibodies; only one asymptomatic subject revealed such antibodies. IgE antibodies were significantly more frequently found in the high‐exposure group (technical centre) when compared with the low‐exposure group (laboratories, experimental animal husbandry). Phytase‐specific IgG antibodies were present in 19 symptomatic (50%) and five (33%) asymptomatic subjects. Conclusions: Our results demonstrate that powdered fungal phytase is a highly sensitizing substance whose inhalation exposure should be avoided. Hypersensitivity symptoms could be prevented by means of extensive hygienic measures and ongoing medical surveillance.
PCSK9, apolipoprotein E and lipoviral particles in chronic hepatitis C genotype 3: Evidence for genotype-specific regulation of lipoprotein metabolism
Authors: Simon H. Bridge, David A. Sheridan, Daniel J. Felmlee, Mary M.E. Crossey, Fiona I. Fenwick, Clare V. Lanyon, Geneviève Dubuc, Nabil G. Seidah, Jean Davignon, Howard C. Thomas, Simon D. Taylor-Robinson, Geoffrey L. Toms, R. Dermot G. Neely, Margaret F. Bassendine
Journal: Journal of Hepatology (2014)
The use of thiolated polymers as carrier matrix in oral peptide delivery—Proof of concept
Authors: Andreas Bernkop-Schnürch, Yvonne Pinter, Davide Guggi, Hermann Kahlbacher, Gudrun Schöffmann, Maximilian Schuh, Ivo Schmerold, Maria Dorly Del Curto, Mauro D'Antonio, Pierandrea Esposito, Christian Huck
Journal: Journal of Controlled Release (2005)
Evolution of Nuclear- and Mitochondrial-Encoded Subunit Interaction in Cytochrome c Oxidase
Authors: Timothy R. Schmidt, Wei Wu, Morris Goodman, Lawrence I. Grossman
Journal: Molecular Biology and Evolution (2001)

Mitochondrial DNA (mtDNA)-encoded proteins function in eukaryotes as subunits of respiratory complexes that also contain nuclear DNA (nDNA)-encoded subunits. The importance of functional interactions between mtDNA- and nDNA-encoded proteins was previously demonstrated by testing the survivability of cybrid cells or individuals containing nDNA and mtDNA from different populations or species. This report focuses on the multisubunit respiratory complex cytochrome c oxidase (COX), made up of both mtDNA-encoded and nDNA-encoded subunits. A combination of evolutionary and crystallographic data is employed to determine whether rates of nonsynonymous substitutions have been higher, the same, or lower for residues in close proximity that are encoded by a different genome (nDNA or mtDNA). This determination is performed by simply taking the ratio, called the interaction ratio i, of the nonsynonymous substitution rate of the close-contact residues to the nonsynonymous substitution rate of the noncontact residues. We assume that the close-contact residues (which are more likely to interact) are functionally important and that, therefore, amino acid replacements among these residues cannot escape the scrutiny of natural selection. i = 1 indicates that the close-contact residues have been under neither greater purifying selection nor greater positive selection than the noncontact residues as a specific consequence of their being encoded by separate genomes. i < 1 indicates that the close-contact residues have been under greater purifying selection but less positive selection than have the noncontact residues. Conversely, i > 1 indicates that the close-contact residues have been under less purifying but greater positive selection than have the noncontact residues. i < 1 may be referred to as a constraining interaction; i.e., the close-contact residues compared with the noncontact residues appear to be under greater structural-functional constraints. On the other hand, i > 1 may be referred to as an optimizing interaction; i.e., apparently many different amino acid replacements are required to optimize this subunit's interaction with the other subunit. A major finding is that the nDNA-encoded residues in close physical proximity to mtDNA-encoded residues evolve more slowly than the other nuclear-encoded residues (and thus display a constraining interaction), whereas the mtDNA-encoded residues in close physical proximity to nDNA-encoded residues evolve more rapidly than the other mitochondrial-encoded residues (and thus display an optimizing interaction). A possible reason for this striking difference between the nuclear- and mitochondrial-encoded COX subunits in how their functional interaction evolves is discussed.
Exhaled breath temperature in asthma: Fig. 1.—
Authors: P. Paredi, S.A. Kharitonov, P.J. Barnes
Journal: European Respiratory Journal (2003)

<h3>Background</h3> Transarterial chemoembolization (TACE) is the therapy which have chosen in 50-60% of patients with Hepatocellular carcinoma (HCC). Recently, Hepatoma Arterial Embolization (HAP) score stratifies patients who will benefit from the first TACE. We conducted a survey to investigate the value of HAP score in the prognosis of HCC treated with TACE in Vietnam. <h3>Methods</h3> Prospective cohort study in patients with HCC and first TACE at the Department of Internal Medicine, Hanoi Medical University Hospital, Vietnam, from January 2017 to June 2021. The HAP score (serum albumin 0.9 mg/dl, alpha-foetoprotein &gt;400 ng/ml, and tumor size &gt;7 cm), and ALBI score was applied before first TACE, mortality and survival were observed with a follow-up of 54 months. <h3>Results</h3> We included 106 patients with age of 62.8 ± 10.1 years. In this population, HCC risk factor was mainly hepatitis B with 75.5% (80/106). The HAP score classified 19, 41, 31 and 15 patients as HAP A, B, C and D, respectively, (IDDF2021-ABS-0070 Table 1). The median follow–up time was 37.5±1.2 months for HAP A, 40.8±2.9 months for HAP B, 24.0±2.5 months for HAP C and 20.4±4.1 months for HAP D. There were no deaths at the first year in HAP A. The cumulative proportion surviving at first years for HAP B, C, D was 97.4; 77.9 and 66.7, respectively. At 36 months, this percentage for HAP A, B, C and D was 92.3; 72.5; 31.1 and 27.1, respectively, (IDDF2021-ABS-0070 Table 2). Survival of all subgroups differed significantly from each other (each p &lt; 0.05) (IDDF2021-ABS-0070 Figure 2. Kaplan-Meier curve for HAP score). Area under the curve for receiver operating characteristic of HAP score was 0.71 and higher than this of ALBI grade (0.57) (IDDF2021-ABS-0070 Figure 3. ROC curves for HAP score and ALBI grade), indicating a significant performance of HAP score compared with ALBI grade in prognosis of HCC treated with TACE. <h3>Conclusions</h3> HAP is a useful score to assist for the management decisions of patients with HCC requiring TACE due to its value in predicting mortality and survival.
NY-ESO-1 Expression and Immunogenicity in Malignant and Benign Breast Tumors
Authors: Yurika Sugita, Hisashi Wada, Shoichiro Fujita, Tetsuya Nakata, Shuichiro Sato, Yuji Noguchi, Achim A. Jungbluth, Masae Yamaguchi, Yao-Tseng Chen, Elisabeth Stockert, Sacha Gnjatic, Barbara Williamson, Matthew J. Scanlan, Toshiro Ono, Isao Sakita, Masayoshi Yasui, Yasuo Miyoshi, Yasuhiro Tamaki, Nariaki Matsuura, Shinzaburo Noguchi, Lloyd J. Old, Eiichi Nakayama, Morito Monden
Journal: Cancer Research (2004)

Abstract NY-ESO-1 is a cancer/testis antigen expressed in normal adult tissues solely in the testicular germ cells of normal adults and in various cancers. It induces specific humoral and cellular immunity in patients with NY-ESO-1-expressing cancer. The aim of this study was to determine the frequency of NY-ESO-1 mRNA and protein expression in malignant and benign breast tumors. NY-ESO-1 mRNA expression was detected by conventional reverse transcription-PCR and real-time PCR, and that of the protein expression by immunohistochemistry and Western blot analysis. Expression of NY-ESO-1 mRNA was detected in 37 of 88 (42%) cancer specimens, whereas that of the NY-ESO-1 protein was detected only in 1 mRNA-positive specimen. In the latter case, expression level of NY-ESO-1 mRNA relative to that in the testis was relatively high (75% of testicular expression) and to the other among breast cancer specimens. In benign breast lesions, 21 of 31 (68%) specimens expressed low levels of NY-ESO-1 mRNA. In 1 case of fibroadenoma, NY-ESO-1 mRNA was 8% of the testicular level, and protein was detected by Western blot analysis. Only 1 breast cancer patient had detectable antibody at time of surgery, which disappeared within 2 years. Tumor specimen from this patient was both NY-ESO-1 mRNA and protein positive, and NY-ESO-1-specific CD8 T cells were detected in this patient by IFN-γ enzyme-linked immunospot assay using NY-ESO-1 recombinant adeno and vaccinia virus. A higher rate of NY-ESO-1 expression was noted in breast cancer with high histological grade and negative hormone receptor status, suggesting NY-ESO-1 as a potential tumor antigen for immunotherapy in patients with breast cancer and poor prognosis.
A STUDY OF THE EFFECT OF INTRAVENOUS INJECTIONS OF SOLUTIONS OF PANCREATIC TISSUE; WITH ESPECIAL REFERENCE TO THE CAUSE OF COLLAPSE IN ACUTE PANCREATITIS
Authors: Anfin Egdahl
Journal: The Journal of Experimental Medicine (1907)

From these results it is permissible to draw the following conclusions which may be used to explain certain phenomena associated especially with the onset of acute pancreatitis. 1. The sudden marked collapse of acute pancreatitis has as its most important cause a toxin or toxins derived from broken down pancreatic tissue. This toxin or toxins seem to be most powerful in the stage of degradation just after the disappearance of the biuret reaction and are probably of the nature of aromatic and amino-compounds. 2. There are various contributing factors of toxic action as peptone and trypsin, but these are to be regarded as of secondary importance. 3. Mechanical irritation as stretching of the peritoneum and irritation of the caeliac plexus is a secondary cause. 4. The glycerine produced through the action of steapsin cannot be regarded as an important cause of collapse.
MicroRNA-124 Controls the Proliferative, Migratory, and Inflammatory Phenotype of Pulmonary Vascular Fibroblasts
Authors: Daren Wang, Hui Zhang, Min Li, Maria G. Frid, Amanda R. Flockton, B. Alexandre McKeon, Michael E. Yeager, Mehdi A. Fini, Nicholas W. Morrell, Soni S. Pullamsetti, Sivareddy Velegala, Werner Seeger, Timothy A. McKinsey, Carmen C. Sucharov, Kurt R. Stenmark
Journal: Circulation Research (2013)

Pulmonary hypertensive remodeling is characterized by excessive proliferation, migration, and proinflammatory activation of adventitial fibroblasts. In culture, fibroblasts maintain a similar activated phenotype. The mechanisms responsible for generation/maintenance of this phenotype remain unknown.We hypothesized that aberrant expression of microRNA-124 (miR-124) regulates this activated fibroblast phenotype and sought to determine the signaling pathways through which miR-124 exerts effects.We detected significant decreases in miR-124 expression in fibroblasts isolated from calves and humans with severe pulmonary hypertension. Overexpression of miR-124 by mimic transfection significantly attenuated proliferation, migration, and monocyte chemotactic protein-1 expression of hypertensive fibroblasts, whereas anti-miR-124 treatment of control fibroblasts resulted in their increased proliferation, migration, and monocyte chemotactic protein-1 expression. Furthermore, the alternative splicing factor, polypyrimidine tract-binding protein 1, was shown to be a direct target of miR-124 and to be upregulated both in vivo and in vitro in bovine and human pulmonary hypertensive fibroblasts. The effects of miR-124 on fibroblast proliferation were mediated via direct binding to the 3' untranslated region of polypyrimidine tract-binding protein 1 and subsequent regulation of Notch1/phosphatase and tensin homolog/FOXO3/p21Cip1 and p27Kip1 signaling. We showed that miR-124 directly regulates monocyte chemotactic protein-1 expression in pulmonary hypertension/idiopathic pulmonary arterial hypertension fibroblasts. Furthermore, we demonstrated that miR-124 expression is suppressed by histone deacetylases and that treatment of hypertensive fibroblasts with histone deacetylase inhibitors increased miR-124 expression and decreased proliferation and monocyte chemotactic protein-1 production.Stable decreases in miR-124 expression contribute to an epigenetically reprogrammed, highly proliferative, migratory, and inflammatory phenotype of hypertensive pulmonary adventitial fibroblasts. Thus, therapies directed at restoring miR-124 function, including histone deacetylase inhibitors, should be investigated.
Seat Belt-Related Thyroiditis Documented with Thyroid Tc-99m Pertechnetate Scans
Authors: ROBERT G. LECKIE, ARTHUR B. BUCKNER, MICHAEL BORNEMANN
Journal: Clinical Nuclear Medicine (1992)

A 64-year-old man presented with clinical unilateral thyroiditis after chronic left neck trauma from his car seat belt. Thyroid function tests were normal. The initial thyroid Tc-99m pertechnetate scan demonstrated decreased uptake in the entire left lobe of the thyroid. The follow-up scan 1 month later showed a more normal uptake in the left lobe. This patient's clinical course, laboratory values, and nuclear medicine scans are compatible with thyroiditis secondary to trauma sustained from his seat belt.
The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity.
Authors: K H Johnston, K K Holmes, E C Gotschlich
Journal: The Journal of Experimental Medicine (1976)

Neisseria gonorrhoeae has been subdivided into several classes of serological distinct groups. The serotyping system is based upon the antigenic specificity of a protein serotype antigen. This protein is the major polypeptide of the outer membrane of the gonococcus and accounts for over 60% of that membrane's total protein. The serotype antigen complex was isolated by mild extraction of intact organisms in 200 mM lithium acetate buffer, pH 6.0 with 10 mM EDTA for 2 h at 45 degrees C. The extract was fractionated on Sepharose 6B and partially purified by precipitation at pH 4.2 by addition of 10% (vol/vol) acetic acid. Each serotype antigen has a unique subunit molecular size as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Preliminary typing of a gonococcal strain may be performed by comparative SDA-PAGE. To date, 16 different serotypes, representing a diverse distribution, have been isolated.
Polymorphisms of the TAP2 transporter gene in systemic lupus erythematosus.
Authors: E J Davies, R P Donn, M C Hillarby, D M Grennan, W E Ollier
Journal: Annals of the Rheumatic Diseases (1994)

OBJECTIVES--To determine whether the TAP2 transporter gene, which lies between HLA-DP and HLA-DQ, is involved in determining susceptibility to systemic lupus erythematosus (SLE). METHODS--TAP2 types were determined by ARMS-PCR in 89 white patients with SLE and 156 control subjects. RESULTS--No particular TAP2 dimorphism or allele was associated with SLE or with any clinical/immunological subgroup of SLE. Furthermore, there was no evidence for significant linkage disequilibrium between TAP2 and HLA-DQ/DR in SLE. CONCLUSIONS--These data suggest that TAP2 is not a disease susceptibility gene for SLE and that the disease-predisposing haplotypes do not extend as far as TAP2. This indicates that any HLA-DP association with SLE must be independent of other class II (DQ/DR) associations.
TRANSFUSION OF RED BLOOD CELLS: THE IMPACT ON SURVIVAL AFTER AORTIC VALVE REPLACEMENT AT TEN-YEAR FOLLOW-UP
Authors: Halit Yerebakan, Robert Sorabella, Marc Najjar, John Vandenberge, Catherine Wang, Hiroo Takayama, Yoshifumi Naka, Michael Argenziano, Mathew Williams, Craig Smith, Isaac George
Journal: Journal of the American College of Cardiology (2014)