tbuckley/cabot-search huggingface dataset:
AB1118 Prevalence of musculoskeletal complaints in 6-17 years old children from india: A school based population study
Authors: B. Abujam, R. Mishra, A. Aggarwal
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Musculoskeletal complaints are common in children in the developed countries. (1) However, most of these are non-inflammatory, and prevalence of juvenile idiopathic arthritis is 7-220 per 100,000. (2) There is limited data from developing countries, including India. Thus, we studied these in Indian school going children. <h3>Objectives</h3> Look at prevalence of musculoskeletal complaints and juvenile idiopathic arthritis in Indian school-children. Also to look for hypermobility and association with musculoskeletal complaints. <h3>Methods</h3> This cross-sectional study was conducted from June 2010-March 2011 among school children aged 6-17 years. Initially, a questionnaire on musculoskeletal complaints was distributed. These had to be filled up by parents in children younger than 14 years of age, but older children could fill it themselves. Subsequently, all children were individually met and their answers verified. Also, a 2nd year rheumatology fellow did an abbreviated musculoskeletal examination (GALS) (3) and checked for hypermobility (Beighton's scoring). <h3>Results</h3> A total of 2059 children (851 girls:1208 boys) were included in the study. Their mean age was 11.5±2.9 yrs. After verification by direct questioning, joint pain (more than 1 week) was present in 158 (7.6%), back pain was present in 63 (3.1%) and heel pain in 62 (3%) children. On examination, 1064 children (51.7%) had hypermobility (Beighton score ≥4), with no gender predilection. Of these, 483 (45.4%) were symptomatic, most with transient joint pains (less than 1 week), a higher prevalence than in those without hypermobility (p&lt;0.001). However none had hypermobility syndrome. None of the children had any evidence of arthritis. Two children had inflammatory back pain, one of whom was confirmed to have enthesitis related arthritis (JIA-ERA) (enthesitis, X-ray showing sacroilitis and HLA-B27 positive). <h3>Conclusions</h3> Musculoskeletal complaints especially joint pain was not uncommon in Indian children. Hypermobility was common, being present in half of the children; also hypermobile children had more musculoskeletal complaints. The estimated prevalence of JIA was 48/100,000 in Indian children. <h3>References</h3> Ziv A, Boulet JR, Slap GB. Utilization of physician offices by adolescents in the United States. Pediatrics. 1999;104:35-42. Manners PJ, Diepeveen DA. Prevalence of juvenile chronic arthritis in a population of 12-year-old children in urban Australia. Pediatrics 1996;98:84-90. Foster HE, Kay LJ, Friswell M, Coady D, Myers A. Musculoskeletal screening examination (pGALS) for school-age children based on the adult GALS screen. Arthritis Rheum. 2006 15;55:709-16. <h3>Disclosure of Interest</h3> None Declared
Abstract 1312: Functional significance for an hnRNP A18 signature RNA motif in the 3'UTR of Ataxia Telangiectasia and RAD3 related (ATR) transcript
Authors: Qingyuan Yang, Ruiqing Yang, Ming Zhan, Narasimharao Nalabothula, France Carrier
Journal: Cancer Research (2010)

Abstract The predominantly nuclear hnRNP A18 translocates to the cytosol in response to cellular stress and increases translation by specifically binding to the 3'untranslated region (UTR) of several mRNA transcripts and the eukaryotic Initiation Factor 4G. Here, we identified a 51 nucleotides motif that is present 11.49 times more often in the 3'UTR of hnRNP A18 mRNA targets than in the unigene data base. This motif was identified by computational analysis of primary sequences and secondary structures of hnRNP A18 mRNA targets against the unaligned sequences. Band shift analyses indicate that the motif is sufficient to confer binding to hnRNP A18. A search of the entire unigene data base indicates that the hnRNP A18 motif is also present in the 3'UTR of the Ataxia Telangiectasia and Rad3 related (ATR) mRNA. Validation of the predicted hnRNP A18 motif is provided by hnRNP A18 binding to ATR 3'UTR and amplification of endogenous ATR transcript on polysomal fractions immunoprecipitated with hnRNP A18 antibodies. Moreover, over expression of hnRNP A18 results in increased ATR protein levels and increased phosphorylation of Chk1, a preferred ATR substrate, in response to UV radiation. To our knowledge, this constitutes the first demonstration of a post-transcriptional regulatory mechanism for ATR activity. hnRNP A18 could thus become a new target to trigger ATR activity as back up stress-response mechanisms to functionally compensate for absent or defective genotoxic stress responders. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1312.
Abstract P1-07-03: Quantification of HER2 expression at the single cell level and HER2 intratumoral heterogeneity of breast cancer tissue samples using automated image analysis
Authors: E Geretti, V Paragas, M Onsum, A Kudla, S Moulis, L Luus, T Wickham, C McDonagh, G MacBeath, B Hendriks
Journal: Cancer Research (2012)

Abstract HER2 is an important biomarker for breast and gastric cancer prognosis and patient treatment decisions. HER2 positivity, as defined by IHC or FISH testing, remains an imprecise predictor of patient response to HER2-targeted therapies. Challenges to accurate HER2 assessment and patient stratification may include intratumoral heterogeneity, lack of assays that are quantitative and/or objective, and differences between measuring HER2 at the protein vs. DNA level. We have developed a novel immunofluorescence method for absolute quantitation of HER2 protein expression at the single cell level on formalin-fixed, paraffin-embedded (FFPE) patient samples. Our assay utilizes automated image analysis software to identify cells, classify them as tumor or non-tumor cells, and quantitate the level of HER2 staining for each tumor cell. The HER2 staining level is then converted to absolute HER2 receptor numbers per cell using an array composed of cell lines that span a range of HER2 levels. This cell pellet array standard is stained in parallel with each tissue sample. With this approach we quantitate HER2 expression at the single cell level and describe the heterogeneity of HER2 expression within breast cancer tissue samples. Our assay provides additional insight into the absolute level of HER2 expression and heterogeneity of HER2 expression within tumors, and allows for direct comparison with the currently existing HER2 assessments by standard IHC and/or FISH. This new assay could be used to increase understanding of the relationship between HER2 expression and patient response to HER2-targeted therapies. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P1-07-03.
Cell-Surface Markers in Lymphoproliferative Disease
Authors: Alan C. Aisenberg
Journal: New England Journal of Medicine (1981)

THE study of lymphocyte-surface markers is central to cellular immunology and is a complex and rapidly changing field.1 , 2 Nonetheless, this is an appropriate time to review the knowledge derived from the conventional techniques of rosette formation and fluorescence microscopy with heteroantiserums while the field pauses before a new body of knowledge is generated with the development of monoclonal antibodies and laser technology (the fluorescence-activated cell sorter). This discussion is restricted to the application of lymphocyte-surface markers to lymphocytic leukemias and lymphomas — a clinical area in which these methods have been useful.3 , 4 At present, the existence of curative treatment for . . .
Books ReceivedThe Child Molester: An Integrated Approach to Evaluation and Treatment
Authors: William J. Shaw
Journal: New England Journal of Medicine (1989)

The development of effective treatment programs to change the behavior of child molesters requires the cooperative efforts of professionals in many fields: the behavioral and biologic sciences, the judiciary, corrections, education, and the social services. Consistent with this view, the book makes an important contribution to the literature.The purpose of this book — as expressed and met by the authors — is to help clinicians treat child molesters. Its clear writing, organization, objectivity, and minimal use of technical language make this book useful for students and members of nonclinical professions.The first two chapters give an overview of the . . .
Abstract 3984: Effect of zeranol on beef skeletal muscle growth by differential image gel electrophoresis (DIGE)
Authors: Macdonald Wick, John M. Reddish, Weiping Ye, Young C. Lin
Journal: Cancer Research (2010)

Abstract Zeranol is a resorcylic acid lactone produced by fungi of the genus Fusarium that grows on corn, wheat, barley, oats and sorghum. As such Zeranol is a virtually unavoidable contaminant of crops used to feed animals that are consumed by humans. Zeranol has been shown to have a positive impact on muscle growth during the finishing phase of beef cattle production, was first approved and licensed for use as a growth promotant in cattle and sheep in the USA by FDA in 1969. However, the mechanism of this interaction is unknown but is likely related to changes in muscle specific proteins observable during the increased growth phase during finishing. To determine the affect of Zeranol on beef cattle muscle growth, we performed four DIGE experiments. We compared protein expression level changes between beef cattle treated with Zeranol and untreated beef cattle. On day zero, 10 cattle were implanted with 72 mg of Zeranol pellets, the other half (n = 10) were implanted with vehicle. The beef cattle were raised at the Ohio State University Department of Animal Sciences Beef Barn in accordance with industry standards for 120 days. At 60 days, half the implanted beef cattle and half of the control beef cattle were harvested and the remaining beef cattle were harvested at day 120. Muscle samples were analyzed by DIGE and bioinformatics Seven proteins associated with energy metabolism (creatine kinase, glycogen phosphorylase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglucomutase I, aconitase 2, enolase I, and triosephophate isomerase), 5 proteins with regulation of muscle contraction (slow skeletal muscle troponin T, calsequestrin I, myosin light chains 2 and 3, tropomyosin 3), 3 structural proteins (alpha actin, myosin binding protein C and kelch repeat and BTB domain), a protein associated with myotube formation, dihydrolipoamide dehydrogenase, and HSP70, a protein associated with meat quality, and albumin were identified. The differential regulation of these proteins indicates that muscle growth in cattle implanted with Z is due to fast skeletal muscle. (Supported by NIH grant R01 ES 015212). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3984.
AB0499 Inflammatory lung disease a potential risk factor for onset of inflammatory myopathies
Authors: I. E. Lundberg, S. Barbasso Helmers, A. L. Wikman, P. Axelman, Å. Lundberg, L. Alfredsson
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background</h3> Although the etiology of inflammatory myopathies is unclear, there are data indicating that myositis occurs as a result of complex interactions between genes and environmental/life style factors. <h3>Objectives</h3> To assess the association between lung disease and the risk of developing inflammatory myopathies, myositis. <h3>Methods</h3> In this population-based case-control study, 179 incident adult myositis cases diagnosed between 1995 and 1997 were identified from the Swedish inpatient registry. Verification of diagnosis was performed through patient records. The analysis was based on responses from a questionnaire on exposures to environmental factors from 100 cases that were alive and fulfilled the inclusion criteria and from 402 randomly selected population controls matched to cases on birth of date, gender and residency. Exposure was based upon self-reports of preceding inflammatory lung disease (pneumonia, tuberculosis or sarcoidosis). The association between inflammatory lung disease and risk of developing myositis was evaluated by calculating odds ratio (OR) together with 95% confidence interval (CI) by means of logistic regression. <h3>Results</h3> There was an association between preceding inflammatory lung disease and diagnosis of myositis: adjusted OR 1.5, 95% CI 0.9-2.6. The median duration between the lung disease and first symptom of myositis was 30 years. <h3>Conclusions</h3> Subjects with preceding inflammatory lung disease had an increased risk of developing myositis compared to those without such disease. Inflammatory lung disease could thus be a potential risk factor for onset of inflammatory myopathies, myositis, or a parallel phenomenon. <h3>Disclosure of Interest</h3> None Declared
Late-Breaking News: FDA Proposes Fat, Cancer Health Claim; Hesitates on Fiber
Authors: E. Taylor, K. Smigel
Journal: JNCI Journal of the National Cancer Institute (1991)

Journal Article Late-Breaking News: FDA Proposes Fat, Cancer Health Claim; Hesitates on Fiber Get access Ellen Taylor, Ellen Taylor Search for other works by this author on: Oxford Academic PubMed Google Scholar Kara Smigel Kara Smigel Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 83, Issue 22, 20 November 1991, Pages 1618–1619, https://doi.org/10.1093/jnci/83.22.1618 Published: 20 November 1991
Measles Encephalomyelitis — Clinical and Immunologic Studies
Authors: Richard T. Johnson, Diane E. Griffin, Robert L. Hirsch, Jerry S. Wounsky, Susi Roedenbeck, Imelda Lindo de Soriano, Abraham Vaisberg
Journal: New England Journal of Medicine (1984)

We studied 19 patients with postinfectious encephalomyelitis complicating natural measles-virus infections, and our results support the hypothesis that this demyelinating disease has a pathogenesis similar to that of experimental allergic encephalomyelitis. Early myelin destruction was demonstrated by the presence of myelin basic protein in cerebrospinal fluid, and lymphocyte proliferative responses to myelin basic protein were found in 8 of 17 patients tested. A lack of intrathecal synthesis of antibody against measles virus suggests that measles encephalomyelitis may not be dependent on virus replication within the central nervous system. Similar lymphoproliferative responses to myelin basic protein of lymphocytes from single patients with encephalomyelitis after rabies vaccine or after varicella or rubella virus infections suggest a common immune-mediated pathogenesis for the perivenular demyelinating disease that can follow the injection of neural tissues or infection by a variety of viruses.
Recall Reaction of a Severe Local Peripheral Neuropathy After Paclitaxel Extravasation
Authors: M. HIDALGO, J. BENITO, R. COLOMER, L. PAZ-ARES
Journal: JNCI Journal of the National Cancer Institute (1996)

Peripheral neuropathy with numbness, tingling, and burning pain in a glove-and-stocking distribution is the most common nonhematologic side effect associated with paclitaxel (Taxol) therapy, whereas nerve damage leading to motor dysfunction is uncommon (J). We report here on a 53-year-old man with both non-insulin-dependent diabetes mellitus without systemic complications and locally advanced non-small-cell lung cancer. The patient was started on paclitaxel that was administered through the right antecubital vein at 175 mg/m2 in a 3-hour infusion (total dose, 360 mg). He also received standard recommended premedication and carboplatin; the carboplatin dose was targeted to an area under the curve for drug concentration as a function of time of 7 (total dose, 1080 mg). During the infusion of paclitaxel, an extravasation of approximately one fifth of the dose occurred. The rest of the paclitaxel dose followed by carboplatin was given through peripheral access in the left arm.
Abstract 3911: Infrared vibrational frequency as a novel molecular descriptor for lead optimization of PKB/Akt inhibitors
Authors: Joel K. Kawakami, Kristin Harrison, Ashley Baldauf, Heidi May
Journal: Cancer Research (2012)

Abstract We report the use of a novel molecular descriptor used for lead optimization of PKB inhibitors. Lead optimization remains a major rate limiting step in drug discovery. Current methods for lead optimization that leads to a clinical candidate often fail during clinical trials with attrition rate as high as 9 out of 10. We re-investigated the lock and key theory by incorporating the vibrational frequency of small molecules. The vibrational frequency is calculated based on the summation of all theoretical infrared vibrational frequency of each bond and normalized via division by its molecular weight. We term this molecular descriptor as MDIR. The MDIR is utilized as a rapid lead optimization computational chemistry method. The MDIR correlation to affinity/inhibition at several molecular targets shows a parabolic relationship. Quinoxalines with MDIR values near 135 provide the best inhibition (19% INH at 10 uM) at PKB/Akt as assessed via the Cisbio HTRF KinEASE assay. An internal control, 10-DEBC, known to have 100 % inhibition of PKB at 2.5 uM, had only a 6 % INH @ 10 uM in the KinEASE assay. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3911. doi:1538-7445.AM2012-3911
Abstract 2144: Characterization of HMGB1-induced cell death in colon carcinoma cells
Authors: Georg Gdynia, Martina Keith, Peter Schirmacher, Wilfried Roth
Journal: Cancer Research (2011)

Abstract The danger protein HMGB1 induces a distinct form of cell death in cancer cells which is different from classical necrosis, apoptosis, autophagy, or senescence. Interestingly, colon carcinoma cells are less sensitive for the cytotoxic activity of HMGB1 than other types of cancer cells. The aim of this study was to characterize the molecular and metabolic pathways regulating the susceptibility to HMGB1 in colorectal carcinoma. Stable ectopic over-expression of HMGB1 inhibited significantly the proliferation of colon carcinoma cells. Treatment of cells with recombinant human (rh) HMGB1 induced significant cytotoxicity in a concentration range of 80 – 200 nM. Cancer cells depleted of mitochondrial DNA were less susceptible to the cytotoxic effects of rhHMGB1. In ATP luciferase assays, after 72 h of incubation with rhHMGB1 a significant depletion of intracellular ATP was observed, paralleled by the formation of giant mitochondria. RhHMGB1 modulated the specific activity of the mitochondrial respiratory enzymes and the electron flux in the electron transport chain in a dose-dependent manner. Interestingly, addition of NAD+, L-pyruvate, or ATP did not protect the cells from rhHMGB1-induced cell death. RhHMGB1-induced mitochondrial and metabolic events were further characterized by tracking radioactive glucose and pyruvate isotopes. In summary, the HMGB1 protein induces a novel form of cell death in diverse types of cancer cells. In colon cancer cells, HMGB1 modulates the activity of the mitochondrial respiratory enzymes leading to a change in glycolytic flux and a strong depletion of intracellular ATP. These observations implicate a new functional link between the HMGB1 protein and mitochondrial respiratory regulation as well as glycolysis activity in cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2144. doi:10.1158/1538-7445.AM2011-2144
Non-toxic Drug Being Tested To Treat Cancer and Anemias
Authors: K. Smigel
Journal: JNCI Journal of the National Cancer Institute (1992)

Journal Article Non-toxic Drug Being Tested To Treat Cancer and Anemias Get access Kara Smigel Kara Smigel Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 84, Issue 18, 16 September 1992, Pages 1398–1399, https://doi.org/10.1093/jnci/84.18.1398 Published: 16 September 1992
Endoscopic ultrasound-guided gastroenterostomy using novel tools designed for transluminal therapy: a porcine study
Authors: K. Binmoeller, J. Shah
Journal: Endoscopy (2012)

<b>Background and study aims: </b>Surgical gastroenterostomy is associated with appreciable morbidity and mortality. We evaluated the technical feasibility and outcomes of a new method of endoscopic ultrasound (EUS)-guided gastroenterostomy using novel tools designed for transluminal therapy. <b>Methods</b>: In one acute and four survival female pigs, a gastroenterostomy was created under EUS guidance. Novel tools used included: (i) an anchor wire; (ii) an access device; (iii) a fully covered metal stent with bilateral lumen-apposing anchors. The anchor guide wire was inserted through a standard 19-G fine needle aspiration (FNA) needle to appose the small-bowel and stomach walls. The access device created a 3.5-mm fistula opening for insertion of the stent delivery catheter. The stent lumen was dilated to 10 mm to pass a gastroscope into the small bowel. <b>Results: </b>The procedure was technically successful in all animals. No bleeding occurred. In one acute animal, necropsy showed good stent position and no tissue injury. In four survival animals, the stents remained fully patent and all animals showed normal eating behavior without signs of infection. Stents were easily removed without tissue trauma at 4.5 weeks (n = 3) or 5.5 weeks (n = 1). After stent removal, the tracts appeared mature and were easily intubated with the gastroscope. Necropsy and histopathology showed complete fusion of the stomach and small-bowel wall layers at the site of gastroenterostomy. <b>Conclusions: </b>EUS-guided gastroenterostomy is feasible using novel tools with no adverse outcomes in a survival porcine model. Further study of this is indicated as an alternative to surgical bypass for the palliation of malignant gastric outlet obstruction in appropriately selected patients.
Oceans and Human Health: Risks and Remedies from the Sea <i>Edited by Patrick J. Walsh, Sharon L. Smith, Lora E. Fleming, Helena M. Solo-Gabriele, and William H. Gerwick</i> St. Louis, MO:Elsevier, Inc. 2008. 672 pp. ISBN: 978-0-12-372584-4, $130 
Authors: D. Jay Grimes
Journal: Environmental Health Perspectives (2009)