tbuckley/cabot-search huggingface dataset:
Early diagnosis of HIV infection in infants by detection of IgA HIV antibodies
Authors: B.J. Weiblen, R. Hoff, F.K. Lee, S. Nesheim, A.J. Nahmias, E.R. Cooper, J.S. Harris, S.I. Pelton, S.H. Landesman, H. Mendez, K. Mcintosh
Journal: The Lancet (1990)
Reduced IL-7 serum titres are associated with progression towards rheumatoid arthritis in less than 6 months inflammatory arthritis
Authors: V. Goeb, P. Aegerter, E. Villeneuve, J. Nam, P. G. Conaghan, P. Fardellone, X. Le Loet, P. Emery, O. Vittecoq, F. Ponchel
Journal: Annals of the Rheumatic Diseases (2011)

<h3>Background</h3> Interleukin (IL-7) is a pleiotropic cytokine that plays a central role in the development and maintenance of T cells, and has been associated with rheumatoid arthritis (RA). Although IL-7 is highly expressed in the joint, we showed that in the serum, levels of IL-7 are reduced in early and established RA patients. We hypothesised that this reduction may have predictive diagnostic value. <h3>Objectives</h3> To determine whether IL-7 titres in serum will identify patients that will evolve towards RA from onset of &lt;6 months. <h3>Methods</h3> 250 patients with inflammatory joint symptoms of &lt;6 months were recruited. Evolution towards RA was monitored over 5 years. 80 healthy controls. IL-7 levels were measured by ELISA. <h3>Results</h3> Expert rheumatologist diagnostic was established over 5 years follow-up: 108 patients developed RA, 20 undifferentiated arthritis (UA), 20 Spondyloarthropathies, 76 other form of rheumatism (including osteoarthritis, CTD, reactive arthritis, gout) and 26 showed no persistence of inflammation. IL-7 at recruitment was reduced significantly only in RA (p&lt;0.009). There was no correlation with any demographic or clinical parameters (age, sex, DAS, CRP, HAQ RF, ACPA, erosion, SE). IL-7 was categorised using the lower limit of the healthy control distribution (10 pg/ml). Using univariate analysis, predictors of RA diagnostic were: ACPA+ (p=0.003), IL-7 &lt;10 pg/ml (p=0.012), CRP (p=0.029), HAQ (0.012), SJC (p=0.021), SE (p=0.031), RF+ (p=0.068). IL-7 levels were however inversely correlated with symptom duration in RA (R= -0.513, p&lt;0.001) but with no other parameter. In multivariate analysis, predictors of RA were: ACPA+ (p=0.001), IL-7&lt;10 pg/ml (p=0.003), SJC (p=0.050). The latest analysis was repeated in the ACPA- only patient (n=193). Predictors were: IL-7&lt;10 pg/ml (p=0.010), DAS (p=0.001), erosion (p=0.050). Remission (DAS&lt;1.6) at 1 year following treatment (HCQ, followed by MTX if required) was only predicted by IL-7&gt;17 pg/ml (upper quartile of IL-7 distribution) at recruitment (p=0.001). A validation cohort is currently being recruited and pilot analysis (n=51) with 12 months follow-up only. Using the American College of Rheumatology-1987 RA criteria, IL-7&lt;10 pg/ml was associated with RA diagnostic (p=0.048, UA=37 and RA=14). Using 2010 EULAR RA criteria, higher IL-7&gt;10 pg/ml was associated with persisting UA (p=0.055, UA_, RA6). <h3>Conclusion</h3> These data demonstrate that a reduction of IL-7 in patient with very recent onset of symptoms has potential as diagnostic biomarkers for evolution towards RA, particularly in ACPA- disease
Inability to Induce Hypertension in Normotensive Rat Expressing AT <sub>1</sub> Receptor Antisense
Authors: Alok S. Pachori, Hongwei Wang, Craig H. Gelband, Carlos M. Ferrario, Michael J. Katovich, Mohan K. Raizada
Journal: Circulation Research (2000)

Abstract —Our previous studies have shown that neonatal delivery of angiotensin type 1 receptor antisense (AT 1 R-AS) in a retroviral vector prevents spontaneously hypertensive rats from developing hypertension for life but has no effect on blood pressure (BP) in normotensive animals. Based on these results, we hypothesized that AT 1 R-AS transduction in normotensive rats would protect them from developing experimental hypertension. The present study was designed to evaluate this hypothesis. A single intracardiac administration of AT 1 R-AS by a retroviral-mediated delivery system (LNSV-AT 1 R-AS) in 5-day-old normotensive Sprague-Dawley rats resulted in long-term expression of the AT 1 R-AS without an effect on basal BP. However, angiotensin II (Ang II)–induced BP, dipsogenic responses, and renovascular contractility were significantly attenuated in the LNSV–AT 1 R-AS–treated rats. Chronic infusion of low-dose Ang II (55 ng · kg − 1 · min − 1 ) in LNSV-alone–treated rats caused a modest increase in BP, profound increase in cardiac hypertrophy, and increased vascular contractility. In contrast, the LNSV-AT 1 R-AS–treated rats were protected from developing these changes after Ang II infusion. These data establish that LNSV-AT 1 R-AS pretreatment protects healthy rats from developing Ang II–dependent hypertension.
Biological Processes Associated with Breast Cancer Clinical Outcome Depend on the Molecular Subtypes
Authors: Christine Desmedt, Benjamin Haibe-Kains, Pratyaksha Wirapati, Marc Buyse, Denis Larsimont, Gianluca Bontempi, Mauro Delorenzi, Martine Piccart, Christos Sotiriou
Journal: Clinical Cancer Research (2008)

Recently, several prognostic gene expression signatures have been identified; however, their performance has never been evaluated according to the previously described molecular subtypes based on the estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2), and their biological meaning has remained unclear. Here we aimed to perform a comprehensive meta-analysis integrating both clinicopathologic and gene expression data, focusing on the main molecular subtypes.We developed gene expression modules related to key biological processes in breast cancer such as tumor invasion, immune response, angiogenesis, apoptosis, proliferation, and ER and HER2 signaling, and then analyzed these modules together with clinical variables and several prognostic signatures on publicly available microarray studies (>2,100 patients).Multivariate analysis showed that in the ER+/HER2- subgroup, only the proliferation module and the histologic grade were significantly associated with clinical outcome. In the ER-/HER2- subgroup, only the immune response module was associated with prognosis, whereas in the HER2+ tumors, the tumor invasion and immune response modules displayed significant association with survival. Proliferation was identified as the most important component of several prognostic signatures, and their performance was limited to the ER+/HER2- subgroup.Although proliferation is the strongest parameter predicting clinical outcome in the ER+/HER2- subtype and the common denominator of most prognostic gene signatures, immune response and tumor invasion seem to be the main molecular processes associated with prognosis in the ER-/HER2- and HER2+ subgroups, respectively. These findings may help to define new clinicogenomic models and to identify new therapeutic strategies in the specific molecular subgroups.
An 8-Week Multicenter, Parallel-Group, Double-Blind, Placebo-Controlled Study of Sertraline in Elderly Outpatients With Major Depression
Authors: Lon S. Schneider, J. Craig Nelson, Cathryn M. Clary, Paul Newhouse, K. Ranga Rama Krishnan, Thomas Shiovitz, Karen Weihs
Journal: American Journal of Psychiatry (2003)

There have been few placebo-controlled trials of selective serotonin reuptake inhibitors for depressed elderly patients. This placebo-controlled study of sertraline was designed to confirm the results of non-placebo-controlled trials.The subjects were outpatients age 60 years or older who had a DSM-IV diagnosis of major depressive disorder and a total score on the 17-item Hamilton Depression Rating Scale of 18 or higher. The patients were randomly assigned to 8 weeks of double-blind treatment with placebo or a flexible daily dose of 50 or 100 mg of sertraline. The primary outcome variables were the Hamilton scale and Clinical Global Impression (CGI) scales for severity and improvement.A total of 371 patients assigned to sertraline and 376 assigned to placebo took at least one dose. At endpoint, the patients receiving sertraline evidenced significantly greater improvements than those receiving placebo on the Hamilton depression scale and CGI severity and improvement scales. The mean changes from baseline to endpoint in Hamilton score were -7.4 points (SD=6.3) for sertraline and -6.6 points (SD=6.4) for placebo. The rate of CGI-defined response at endpoint was significantly higher for sertraline (45%) than for placebo (35%), and the time to sustained response was significantly shorter for sertraline (median, 57 versus 61 days). There were few discontinuations due to treatment-related adverse events, 8% for sertraline and 2% for placebo.Sertraline was effective and well tolerated by older adults with major depression, although the drug-placebo difference was not large in this 8-week trial.
THE EFFECT OF 10 MG. OF BENZEDRINE SULFATE ON THE OTIS TEST SCORES OF COLLEGE STUDENTS
Authors: J. E. BARMACK
Journal: American Journal of Psychiatry (1940)

Forty-two male college students were divided into two groups. One group was given 10 mg. of benzedrine sulfate, and the other a placebo at the first session. They were then given a form of the Otis self-administering tests of mental ability (higher form). One week later, the groups were rotated and tested with an alternate form. Under the conditions of the experiment, we found that: 1. The 10 mg. of benzedrine were without a favorable effect on the Otis test score. 2. A small but statistically significant practice effect was noted upon retesting.
Ultrastructural features resembling those of harlequin ichthyosis in patients with severe congenital ichthyosiform erythroderma.
Authors: E. Virolainen, K-M. Niemi, A. Ganemo, J. Kere, A. Vahlquist, U. Saarialho-Kere
Journal: British Journal of Dermatology (2001)

Congenital ichthyoses are a group of heterogeneous disorders of cornification. Autosomal recessive congenital ichthyosis (ARCI) can be clinically subdivided into congenital ichthyosiform erythroderma and lamellar ichthyosis. Ultrastructurally, ARCI is classified into four groups: ichthyosis congenita (IC) types I-IV. The genetic background of the ARCI disorders is heterogeneous, but only one disease gene, transglutaminase 1, has been detected so far. We describe six patients with severe congenital ichthyosis from six different Scandinavian families. They could not be classified ultrastructurally into the four IC groups because of atypical findings of electron microscopy. These included abnormal lamellar bodies, alterations in keratohyalin, remnant organelles and lipid inclusions in the upper epidermal cells, which resembled the ultrastructural findings of harlequin ichthyosis (HI), although the HI phenotype was not present at birth. Some clinical features, such as thick scales, erythroderma, alopecia and ectropion were common to all patients. Ichthyosis was usually accentuated in the scalp and four patients had clumped fingers and toes. None of the patients carried the transglutaminase 1 mutation. We conclude that ultrastructural findings resembling those detected in previous HI cases (type 1 and 2) can also be found in patients who do not have classic clinical features of that rare ichthyosis. This may be due to lack of specificity of ultrastructural markers for HI or to its clinical heterogeneity.
Telomere DNA Content Predicts Breast Cancer–Free Survival Interval
Authors: Christopher M. Heaphy, Kathy B. Baumgartner, Marco Bisoffi, Richard N. Baumgartner, Jeffrey K. Griffith
Journal: Clinical Cancer Research (2007)

Abstract Background: Telomeres are nucleoprotein complexes that protect chromosome ends from degradation and recombination. Critically shortened telomeres generate genomic instability. It has been postulated that the extent of telomere DNA loss is related to the degree of genomic instability within a tumor and therefore may presage clinical outcome. The objective of this investigation was to evaluate the hypothesis that telomere DNA content (TC) in breast tumor tissues predicts breast cancer–free survival interval. Materials and Methods: Slot blot titration assay was used to quantitate TC in 530 archival breast tumor tissues in a population-based cohort. The relationships between TC, 12 risk factors for breast cancer adverse events (i.e., death due to breast cancer, breast cancer recurrence, or development of a new primary breast tumor), and breast cancer–free survival interval were evaluated by Fisher's exact test, log-rank analysis, and univariate and multivariate Cox proportional hazards models. Results: TC was independent of each of the 12 risk factors. Ethnicity, tumor-node-metastasis stage, estrogen receptor, progesterone receptor, and p53 status, chemotherapy sequence, adjuvant therapy, and TC each conferred significant relative hazards. The best overall multivariate Cox proportional hazards model included TC, p53 status, tumor-node-metastasis stage, and estrogen receptor status as independent predictors of breast cancer–free survival interval (P &amp;lt; 0.00005). Low TC (≤200% of standard), relative to the high-TC group (&amp;gt;200% of standard), conferred an adjusted relative hazard of 2.88 (95% confidence interval, 1.16-7.15; P = 0.022) for breast cancer–related adverse events. Conclusions: TC in breast cancer tissue is an independent predictor in this group of breast cancer–free survival interval.
Body composition in sport: interobserver reliability of a novel ultrasound measure of subcutaneous fat tissue
Authors: Wolfram Müller, Martin Horn, Alfred Fürhapter-Rieger, Philipp Kainz, Julia M Kröpfl, Timothy R Ackland, Timothy G Lohman, Ronald J Maughan, Nanna L Meyer, Jorunn Sundgot-Borgen, Arthur D Stewart, Helmut Ahammer
Journal: British Journal of Sports Medicine (2013)

Background Very low body mass, extreme mass changes, and extremely low per cent body fat are becoming increasingly common in many sports, but sufficiently reliable and accurate field methods for body composition assessment in athletes are missing. Methods Nineteen female athletes were investigated (mean (SD) age: 19.5 (±3.3) years; body mass: 59.6 (±7.6) kg; height: 1.674 (±0.056) m; BMI: 21.3 (±2.3) kg/m 2 ). Three observers applied diagnostic B-mode-ultrasound (US) combined with the evaluation software for subcutaneous adipose tissue measurements at eight ISAK sites (International Society for the Advancement of Kinanthrometry). Regression and reliability analyses are presented. Results US measurements and evaluation of subcutaneous adipose tissue (SAT) thicknesses (including fibrous structures: D included ; n=378) resulted in an SE of estimate SEE=0.60 mm, R 2 =0.98 (p&lt;0.001), limit of agreement LOA=1.18, ICC=0.968 (0.957–0.977). Similar values were found for D excluded : SEE=0.68 mm, R 2 =0.97 (p&lt;0.001). D included at individual ISAK sites: at biceps , R 2 =0.87 and intraclass-correlation coefficient ICC=0.811 were lowest and SEE=0.79 mm was highest. Values at all other sites ranged from R 2 : 0.94–0.99, SEE: 0.42–0.65 mm, and ICC: 0.917–0.985. Interobserver coefficients ranged from 0.92 to 0.99, except for biceps (0.74, 0.83 and 0.87). Evaluations of 20 randomly selected US images by three observers (D included ) resulted in: SEE=0.15 mm, R 2 =0.998(p&lt;0.001), ICC=0.997 (0.993, 0999). Conclusions Subject to optimal choice of sites and certain standardisations, US can offer a highly reliable field method for measurement of uncompressed thickness of the SAT. High accuracy and high reliability of measurement, as obtained with this US approach, are essential for protection of the athlete’s health and also for optimising performance.
THU0628-HPR Lifestyle Habits Relates to Quality of Life in Patient with Longstanding Rheumatoid Arthritis
Authors: K. Malm, B. Arvidsson, M. Andersson, S. Bergman, A. Bremander, I. Larsson
Journal: Annals of the Rheumatic Diseases (2015)

<h3>Background</h3> Fatigue, pain, stiffness, impaired muscle function and impaired physical function are some of the most pronounced symptoms in rheumatoid arthritis (RA) and these may be related to lifestyle habits such as physical activity, diet, smoking and alcohol. There is limited knowledge about how patient with longstanding RA understand their lifestyles habits in relation to their disease and quality of life. <h3>Objectives</h3> To describe experiences of how lifestyle habits relate to quality of life in patients with longstanding RA. <h3>Methods</h3> A qualitative study with a deductive content analysis design, including 17 patients from the Swedish BARFOT (Better Anti-Rheumatic FarmacoTherapy) cohort. BARFOT is a long time follow up study of early RA. Informants were strategically selected by gender (ten women and seven men), age (range 30-84 years), disease duration (8-23 years), function as measured by HAQ, and quality of life as measured by EQ5D. Semi-structured interviews focused on four lifestyle habits (main categories); Physical activity, Diet, Smoking, and Alcohol. The interviews were recorded, transcribed verbatim and coded into subcategories within each of the four main categories. <h3>Results</h3> In patients with longstanding RA quality of life was related to the four given main categories (lifestyle habits). Each main category included two to three subcategories; (1) Physical activity means barrier, opportunities and well-being, (2) Diet means shame, well-being and social relationship, (3) Smoking means reward and fear, and (4) Alcohol means ambivalence and social relationship. <h3>Conclusions</h3> In longstanding RA, lifestyle habits relates to quality of life through both positive and negative experiences. This has to be taken into account in clinical care for a better understanding of how patients conceive and adherer to advice on lifestyle. <h3>References</h3> Scott DL, Wolfe F, Huizinga TW. Rheumatoid arthritis. Lancet. 2010;376(9746):1094-108. Hsieh HF, Shannon SE. Three approaches to qualitative content analysis. Qualitative health research. 2005;15(9):1277-88. <h3>Acknowledgements</h3> <h3>Disclosure of Interest</h3> None declared
Transcription factors Foxo3a and Foxo1 couple the E3 ligase Cbl-b to the induction of Foxp3 expression in induced regulatory T cells
Authors: Yohsuke Harada, Yasuyo Harada, Chris Elly, Ge Ying, Ji-Hye Paik, Ronald A. DePinho, Yun-Cai Liu
Journal: The Journal of Experimental Medicine (2010)

The transcription factor Foxp3 is essential for optimal regulatory T (T reg) cell development and function. Here, we show that CD4+ T cells from Cbl-b RING finger mutant knockin or Cbl-b–deficient mice show impaired TGF-β–induced Foxp3 expression. These T cells display augmented Foxo3a phosphorylation, but normal TGF-β signaling. Expression of Foxo3a rescues Foxp3 expression in Cbl-b–deficient T cells, and Foxo3a deficiency results in defective TGF-β–driven Foxp3 induction. A Foxo3a-binding motif is present in a proximal region of the Foxp3 promoter, and is required for Foxo3a association. Foxo1 exerts similar effects as Foxo3a on Foxp3 expression. This study reveals that Foxo factors promote transcription of the Foxp3 gene in induced T reg cells, and thus provides new mechanistic insight into Foxo-mediated T cell regulation.
Disruption of phospholipase C 1 signalling attenuates cardiac tumor necrosis factor- expression and improves myocardial function during endotoxemia
Authors: T. Peng, E Shen, J. Fan, Y. Zhang, J. M. O. Arnold, Q. Feng
Journal: Cardiovascular Research (2007)

Lipopolysaccharide (LPS) induces tumor necrosis factor-α (TNF-α) expression in cardiomyocytes, which contributes to myocardial dysfunction during sepsis. The purpose of this study was to investigate the role of phosphatidylinositol (PI) phospholipase Cγ1 (PLCγ1) in cardiac TNF-α expression, and myocardial dysfunction during endotoxemia. In cultured mouse neonatal cardiomyocytes, LPS increased PLCγ1 phosphorylation. Knockdown of PLCγ1 with specific siRNA or inhibition of PLCγ1 with U73122 attenuated TNF-α expression induced by LPS. This action of PLCγ1 was mediated through inositol-1,4,5-trisphosphate (IP3)/IP3 receptor (IP3R) pathways since blocking either IP3 or IP3R decreased LPS-induced TNF-α expression. In contrast, neither diacylglycerol agonist nor antagonist had any evident effect on LPS-induced TNF-α expression in cardiomyocytes. To investigate the role of PLCγ1 in endotoxemia in vivo, wild-type and heterozygous PLCγ1 knockout (PLCγ1+/−) mice were pre-treated with either U73122, or its inactive analog U73343, or vehicle for 15 min, followed by LPS for 4 h. Inhibition of PLCγ1 by U73122 or by heterozygous deletion of the PLCγ1 gene decreased cardiac TNF-α expression. More importantly, LPS-induced myocardial dysfunction was also attenuated in PLCγ1+/− mice or by U73122 treatment. PLCγ1 signalling induces cardiac TNF-α expression and myocardial dysfunction during LPS stimulation. The action of PLCγ1 on TNF-α expression is mediated through IP3/IP3R pathways. The present results suggest that PLCγ1 may be a potential therapeutic target for myocardial dysfunction in sepsis.
Localization of ADAMTS13 to the stellate cells of human liver
Authors: Masahito Uemura, Kouko Tatsumi, Masanori Matsumoto, Masao Fujimoto, Tomomi Matsuyama, Masatoshi Ishikawa, Taka-aki Iwamoto, Toshio Mori, Akio Wanaka, Hiroshi Fukui, Yoshihiro Fujimura
Journal: Blood (2005)
RETURN-TO-PLAY CRITERIA AFTER HAMSTRING INJURY: ACTUAL MEDICINE PRACTICE IN PROFESSIONAL SOCCER TEAMS
Authors: François Delvaux, Pierre Rochcongar, Olivier Bruyère, Guillaume Bourlet, Christophe Daniel, Pierre Diverse, Jean-Yves Reginster, Jean-Louis Croisier
Journal: British Journal of Sports Medicine (2013)

Background Return-to-play (RTP) criteria after a sport injury represent a key point in order to decrease the risk of subsequent re-injury. Objective To analyze how clinicians decide, in their daily practice, when a hamstring injured professional soccer player is fully able to get back to competitive activities. Methods Thirty-seven doctors for French and Belgian professional soccer clubs filled in a questionnaire formulated with multiple choice questions. Results Nearly 80% of the questioned doctors declared to use at least 7 criteria in order to assess player's ability to return to competitive soccer after a hamstring injury. The most important ones are: complete pain relief, normalized muscle strength assessment, subjective feeling reported by the player, normalized flexibility and achievement of a specific soccer test. For some of these criteria, most notably muscle strength assessment, there is a lack of consensus about the choice of assessment parameters and the limit value allowing doctors to authorize or forbid RTP. Our study also highlighted the interest for clinicians to consider the opinion of physical therapists and physical trainers. Conclusion After a hamstring injury, clinicians for professional soccer clubs assess player's ability to return to full sport according to a “criteria-approach” instead of a “timed-based” approach. However, even if the scientific evidence about this topic is not sufficient at the moment, the implementation, in daily practice, of scientifically demonstrated assessment parameters and limit values should help the RTP decision and decrease the risk of re-injury.