tbuckley/cabot-search huggingface dataset:
Establishment of the DU.528 human lymphohemopoietic stem cell line.
Authors: J Kurtzberg, S H Bigner, M S Hershfield
Journal: The Journal of Experimental Medicine (1985)

We have established the DU.528 cell line from the pretreatment leukemia cells of a patient who underwent a T lymphoblastic-to-promyelocytic phenotype conversion during treatment with the adenosine deaminase inhibitor, deoxycoformycin. The cell line and clones obtained from it by limiting dilution have the same karyotype previously found in the patient's pretreatment T lymphoblasts and post-deoxycoformycin treatment promyelocytes. DU.528 cells in continuous culture for greater than 2 yr display a predominant undifferentiated T lymphoblastoid phenotype. These cells spontaneously generate progeny of at least three lineages, T lymphoid, granulocytic/monocytic, and erythroid. The surface marker most consistently expressed by DU.528 cells in the undifferentiated state is the 3A1 antigen, which has been found on prothymocytes in the embryonic thymus. Some undifferentiated DU.528 cells also expressed the IL-2 receptor, but no other T cell differentiation antigens. Exposure of DU.528 cells to a variety of agents induced myeloid maturation; adenosine and deoxyadenosine, in the presence of deoxycoformycin, induced expression of myeloid differentiation antigens. Our results suggest that DU.528 is a lymphohematopoietic stem cell line and support the hypothesis that differentiation of pluripotent stem cells may be altered by genetic deficiency of adenosine deaminase. DU.528 cells may provide a useful model for examining factors that regulate stem cell proliferation and differentiation.
Role of vascular cell adhesion molecule 1/very late activation antigen 4 and intercellular adhesion molecule 1/lymphocyte function-associated antigen 1 interactions in antigen-induced eosinophil and T cell recruitment into the tissue.
Authors: H Nakajima, H Sano, T Nishimura, S Yoshida, I Iwamoto
Journal: The Journal of Experimental Medicine (1994)

To determine the role of vascular cell adhesion molecule 1 (VCAM-1)/very late activation antigen 4 (VLA-4) and intercellular adhesion molecule 1 (ICAM-1)/lymphocyte function-associated antigen 1 (LFA-1) interactions in causing antigen-induced eosinophil and T cell recruitment into the tissue, we studied the effect of the in vivo blocking of VCAM-1, ICAM-1, VLA-4, and LFA-1 by pretreatment with monoclonal antibodies (mAb) to these four adhesion molecules on the eosinophil and T cell infiltration of the trachea induced by antigen inhalation in mice. The in vivo blocking of VCAM-1 and VLA-4, but not of ICAM-1 and LFA-1, prevented antigen-induced eosinophil infiltration into the mouse trachea. On the contrary, the in vivo blocking of VCAM-1 and VLA-4, but not of ICAM-1 and LFA-1, increased blood eosinophil counts after antigen challenge, but did not affect blood eosinophil counts without antigen challenge in sensitized mice. Furthermore, the expression of VCAM-1 but not ICAM-1 was strongly induced on the endothelium of the trachea after antigen challenge. In addition, pretreatment with anti-IL-4 mAb decreased the antigen-induced VCAM-1 expression only by 27% and had no significant effect on antigen-induced eosinophil infiltration into the trachea. The in vivo blocking of VCAM-1 and VLA-4 inhibited antigen-induced CD4+ and CD8+ T cell infiltration into the trachea more potently than that of ICAM-1 and LFA-1. In contrast, regardless of antigen challenge, the in vivo blocking of LFA-1, but not of ICAM-1, increased blood lymphocyte counts more than that of VCAM-1 and VLA-4. These results indicate that VCAM-1/VLA-4 interaction plays a predominant role in controlling antigen-induced eosinophil and T cell recruitment into the tissue and that the induction of VCAM-1 expression on the endothelium at the site of allergic inflammation regulates this eosinophil and T cell recruitment.
Burrowing bacteria may explain recurrent urinary tract infections
Authors: A. Berger
Journal: BMJ (1998)

People who have recurrent urinary tract infections may have a chronic infection that is never fully eradicated, rather than having a new infection each time. Scientists have discovered that a strain of Escherichia coli that commonly causes urinary tract infections is able to burrow its way into the deeper tissue of the bladder. This may enable the bacteria to avoid the bladder's natural defences and resist eradication by antibiotics, potentially explaining why many people have recurrent infections …
Assault Weapons as a Public Health Hazard-Reply
Authors: Sona Kalousdian
Journal: JAMA (1992)

<h3>In Reply.</h3> —While we certainly appreciate the views of Drs Noel, Di Maio, and Belding concerning the American Medical Association Council on Scientific Affairs Report, "Assault Weapons as a Public Health Hazard in the United States," we believe a statement from the California Attorney General's Office quoted in the article bears repeating: "The danger of the semiautomatic assault weapon is not yet the frequency with which it is used in crime or the volume which exist compared to other types of guns. Rather the danger is the potential each semiautomatic weapon confers upon an individual to kill and wound a large number of people."<sup>1</sup>
METHODS OF DIAGNOSIS AND THERAPEUTICS OF DISEASES OF THE STOMACH AND INTESTINES.
Authors: FENTON B. TURCK
Journal: JAMA (1895)

Diagnosis based upon a group of symptoms only is of little significance in determining pathologic conditions, and, therefore, is of no decided value in rational therapeutics. However, symptoms and history must not be ignored. When a group of symptoms present themselves with a history pointing to a gastric disorder, we must depend upon direct examination to complete a diagnosis. It has always been my custom to make a general physical examination for the purpose of establishing a diagnosis in gastric diseases. I begin with the hair, continue with the eyes, nose, mouth and skin, the general appearance of the body, and especially the contour and configuration of the abdomen. I use boundary lines which connect fixed points; the median line from the interclavicular notch to the symphysis pubis; the parasternal lines (parallel to the median) from the junction of the middle and inner third of the clavicle, to Poupart's ligament;
Oxfam must shed its ideological bias to be taken seriously
Authors: D. Montague
Journal: BMJ (2009)

Oxfam has called for better information on the role of the private sector in health care in developing countries, while simultaneously demanding that the World Bank, bilateral donors, and private foundations cease to support programmes or policies that would work with the private sector.1 Oxfam bases this call on data that are thin, selective, and distorted.

Oxfam notes on the basis of an unpublished study that in 44 middle and low income …
GOITER IN THE INTERMOUNTAIN REGION OF UTAH
Authors: GEORGE W. MIDDLETON
Journal: JAMA (1925)

The larger phase of the goiter problem, and the one that may prove to be most significant in the future, has not yet been touched on. It is the relative iodin deficiency in the various geological strata that form the catchment basins and water sheds of the streams. If this were determined in any given section, one could at a glance estimate the probable goiter incidence and the extent of prophylactic measures necessary to meet the situation. Since iodin is a volatile substance, we would naturally expect that the igneous rocks and the springs of water that come from them would be absolutely iodin free. Theoretically, all volcanic or granitic sections of country should have a relatively large percentage of goiter incidence. Whether this is the case or not remains to be proved, but in the data available in this intermountain region there is at least a marked indication in
Climate and Chest Disorders in Schoolchildren
Authors: K. J. Cullen
Journal: BMJ (1972)

A survey of the respiratory disorders in schoolchildren from rural areas showed no significant differences between the United Kingdom and Australia and New Zealand in loss of time from school owing to chest illness. Contrary to expectations the warmer the region the greater the prevalence of bronchitis, asthma, and hay fever. These results should encourage a critical appraisal of the respiratory benefits of warmer climates, and also suggest that the English climate should prove to be no more than a minor barrier to any future reduction of respiratory mortality in the U.K.
Stretch-induced atriopeptin secretion in the isolated rat myocyte and its negative modulation by calcium.
Authors: J E Greenwald, M Apkon, K A Hruska, P Needleman
Journal: Journal of Clinical Investigation (1989)

Cellular mechanism(s) regulating atriopeptin secretion and processing by the atrial myocyte are currently unknown. Osmotic stretch of isolated atrial myocytes as well as potassium chloride depolarization were potent stimuli of atriopeptin secretion. Release was potentiated by buffering either extracellular calcium with EGTA or intracellular calcium with the intracellular chelator, BAPTA AM. Atrial release of atriopeptin was inhibited after administration of ionomycin which elevates intracellular calcium. Fetal or early neonatal ventricular myocytes actively synthesize atriopeptin. Atriopeptin secretion by ventricular myocytes was also markedly potentiated by osmotic stretch as well as KCl depolarization. Only the 126 amino acid prohormone was secreted by the stretch-stimulated atrial and ventricular myocyte. These data suggest that stretch of the myocyte plasma membrane is a major stimulus for atriopeptin secretion and that atriopeptin secretion is not stimulated by raising intracellular calcium and appears to be negatively modulated by this cation. Like the atrial myocyte, the ventricular myocyte possesses the cellular mechanism(s) necessary to secrete atriopeptin by a regulated mechanism.
Urinary Incontinence Treated with Thioridazine
Authors: Douglas R. Bey
Journal: JAMA (1972)

To the Editor.—  The LETTERS by Renshaw ( 218 :738, 1971) and Crittenden ( 219 :217, 1972) reporting cases of urinary incontinence associated with the use of thioridazine have inspired me to report a case in which urinary symptoms including incontinence were treated using high doses of thioridazine.  The patient was a woman in her 40s who had visited several physicians between 1950 and 1964 usually because of recurrent urethral and bladder infections, with the usual complaints. She was periodically treated with appropriate antibiotics and often physical and laboratory examination.  From 1964 to 1970, her complaints continued but were accompanied by an increase in discomfort and desperation on her part. She visited a number of physicians during this period, including three urologists. Her bladder capacity was noted to be diminished (measured variously from 400 to slightly over 600 ml) and a urethral stricture was dilated and later repaired with a meatoplasty. Later
Malnutrition and Mortality in Kohistan District, Afghanistan, April 2001
Authors: Fitsum Assefa
Journal: JAMA (2001)

The humanitarian situation in Afghanistan has been deteriorating for more than 3 years because of civil war and severe drought. Because of recent events, the international community is predicting a severe worsening of the country's current situation.To assess the magnitude and causes of mortality and prevalence of malnutrition in Kohistan district, Faryab province, Afghanistan.Two-stage, 30-cluster household survey conducted April 4 through 10, 2001, which included anthropometric measurements, assessment of food coping mechanisms, and retrospective mortality data collection for November 26, 2000, through April 4, 2001.A total of 378 households comprising 3165 people living in Kohistan district, Faryab province, Afghanistan.Crude mortality rate, mortality rate among children younger than 5 years, causes of death, prevalence of wasting and stunting among children aged 6 to 59 months (weight-for-height and height-for-age z scores <-2, respectively), and food coping mechanisms.The crude mortality rate among the 3165 persons surveyed during the period of interest was 2.6 (95% confidence interval [CI], 1.7-3.5) per 10 000 per day and the mortality rate among 763 children younger than 5 years was 5.9 (95% CI, 2.0-8.8) per 10 000 per day. Diarrhea (25.0%), respiratory tract infections (19.4%), measles (15.7%), and scurvy (6.5%) caused most of the 108 deaths. The prevalences of wasting and stunting among 708 children aged 6 to 59 months were 7.0% (95% CI, 5.9%-9.0%) and 63.7% (95% CI, 58.6%-68.8%), respectively. Evidence of late-stage food coping mechanisms and prefamine indicators existed among the population.These data indicate that, by April 2001, a humanitarian crisis already existed in Kohistan. Essential humanitarian services, including food aid and public health programs, are urgently required in such regions of Afghanistan and will be crucial if a worsening humanitarian crisis is to be avoided. For these services and programs to be implemented, the international community needs to create adequate humanitarian space (ie, a secure and accessible location where humanitarian organizations can provide services to emergency-affected populations) to ensure that humanitarian organizations have access to populations within Afghanistan as well as to refugees who flee to surrounding countries.
Epac2 induces synapse remodeling and depression and its disease-associated forms alter spines
Authors: Kevin M Woolfrey, Deepak P Srivastava, Huzefa Photowala, Megumi Yamashita, Maria V Barbolina, Michael E Cahill, Zhong Xie, Kelly A Jones, Lawrence A Quilliam, Murali Prakriya, Peter Penzes
Journal: Nature Neuroscience (2009)
Apolipoprotein(B) identifies dyslipidemic phenotypes associated with cardiovascular risk in normocholesterolemic type 2 diabetic patients.
Authors: A M Wägner, A Pérez, F Calvo, R Bonet, A Castellví, J Ordóñez
Journal: Diabetes Care (1999)

Apolipoprotein(B) [apo(B)] reflects the total mass of atherogenic particles (VLDL, IDL, and LDL), and its increase is associated with cardiovascular disease independently of LDL cholesterol (LDLc) levels. Apo(B) determination has been recently standardized, but attention to regional reference limits is advisable. Our aim was to analyze the frequency of dyslipidemic phenotypes, including those dependent on increased apo(B) in normocholesterolemic type 2 diabetic patients.A total of 100 consecutively seen type 2 diabetic patients (63 men, 37 women; aged 59 +/- 11 years) were included, after excluding those on lipid-lowering therapy. Apo(B) cutoff (1.1 g/l) was obtained from a group of normolipidemic (47 men, 21 women) control subjects, and LDLc, triglycerides, and HDL cholesterol (HDLc) cutoff points were those from the National Cholesterol Education Program guidelines. LDLc levels were obtained by ultracentrifugation if triglyceride levels were > 3.45 mmol/l; otherwise, they were calculated (Friedwald). Apo(B) levels were measured by immunoturbidimetry.Normocholesterolemia (LDLc < 4.13 mmol/l) appeared in 75 of the 100 patients, of whom 55 were normo- and 20 hypertriglyceridemic. Hyperapolipoprotein(B) [hyperapo(B)] was the most frequent lipid disorder, present in 34 (45%) of the normocholesterolemic patients (22 normo- and 12 hypertriglyceridemic). Low HDLc levels were more prevalent (53%) in patients with hyperapo(B) than in the rest (24%).Hyperapo(B) was found in almost half of the normocholesterolemic type 2 diabetic patients and was frequently associated with low HDLc levels and hypertriglyceridemia. Thus, given its independent association with cardiovascular disease and that it identifies high-risk phenotypes in normocholesterolemic diabetic patients apo(B) should be used to evaluate the lipidic pattern of these patients.
Remodelling and functional alterations of the rabbit coronary artery in volume overloaded heart
Authors: M Gerova, A Holecyova, F Kristek, A Fizel', A Fizel'ova
Journal: Cardiovascular Research (1993)

Journal Article Remodelling and functional alterations of the rabbit coronary artery in volume overloaded heart Get access M Gerová, M Gerová Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia: M Gerová, A Holécyová, F Kristek; Medical School, Comenius University, Bratislava, Slovakia: A Fízel', A Fízel'ová. Correspondence to Dr Gerová. Search for other works by this author on: Oxford Academic PubMed Google Scholar A Holécyová, A Holécyová Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia: M Gerová, A Holécyová, F Kristek; Medical School, Comenius University, Bratislava, Slovakia: A Fízel', A Fízel'ová. Search for other works by this author on: Oxford Academic PubMed Google Scholar F Kristek, F Kristek Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia: M Gerová, A Holécyová, F Kristek; Medical School, Comenius University, Bratislava, Slovakia: A Fízel', A Fízel'ová. Search for other works by this author on: Oxford Academic PubMed Google Scholar A Fízel', A Fízel' Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia: M Gerová, A Holécyová, F Kristek; Medical School, Comenius University, Bratislava, Slovakia: A Fízel', A Fízel'ová. Search for other works by this author on: Oxford Academic PubMed Google Scholar A Fízel'ová A Fízel'ová Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia: M Gerová, A Holécyová, F Kristek; Medical School, Comenius University, Bratislava, Slovakia: A Fízel', A Fízel'ová. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 27, Issue 11, November 1993, Pages 2005–2010, https://doi.org/10.1093/cvr/27.11.2005 Published: 01 November 1993 Article history Received: 06 April 1993 Accepted: 06 July 1993 Published: 01 November 1993
The principle of double effect and medical ethics.
Authors: R Gillon
Journal: BMJ (1986)

In one of a series of articles on philosophical medical ethics, Gillon examines the Roman Catholic doctrine of double effect as it applies to discussions of the moral difference between acts and omissions in patient care. The doctrine holds that, in the context of actions that have both good and bad effects, an action that has a bad effect is morally permissible if (a) the action itself is good, (b) its perpetrator's intention is solely to produce the good effect, (c) the good effect is not achieved through the bad, and (d) there is sufficient reason to permit the bad effect. Gillon concludes that, while the doctrine of double effect is unlikely to be accepted fully by non-absolutists, some of its claims are useful in deciding which clinical interventions are morally justified.
Role of endothelium-derived nitric oxide in the bleeding tendency of uremia.
Authors: G Remuzzi, N Perico, C Zoja, D Corna, D Macconi, G Viganò
Journal: Journal of Clinical Investigation (1990)

Endothelium-derived relaxing factor, now identified as nitric oxide (NO), is a labile humoral agent formed by vascular endothelial cells from L-arginine. NO mediates the action of substances that induce endothelium-dependent relaxation and plays a role in regulating blood pressure. In this study we investigated whether NO is involved in the pathogenesis of the bleeding tendency associated with renal failure. Rats with extensive surgical ablation of renal mass develop renal insufficiency due to progressive glomerulosclerosis. Like uremic humans, rats with renal mass reduction and uremia have a bleeding tendency that manifests itself by a prolonged bleeding time. We found that N-monomethyl-L-arginine (L-NMMA), a specific inhibitor of NO formation from L-arginine, completely normalized bleeding time when given to uremic rats. L-NMMA injection also increased ex vivo platelet adhesion but did not affect ex vivo platelet aggregation induced by adenosine diphosphate, arachidonic acid, and calcium ionophore A23187. The shortening effect of L-NMMA on bleeding time was completely reversed by giving the animals the NO precursor L-arginine, but not D-arginine, which is not a precursor of NO. It thus appears that NO is a mediator of the bleeding tendency of uremia.
Intensive Insulin Therapy in Hospitalized Patients
Authors: Lynn Bentson
Journal: Annals of Internal Medicine (2011)

Letters21 June 2011Intensive Insulin Therapy in Hospitalized PatientsLynn Bentson, MDLynn Bentson, MDFrom Samaritan Health Physicians, Albany, OR 97321.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-154-12-201106210-00018 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:I appreciated Qaseem and colleagues' extensive review of in-hospital glucose targets (1). Perhaps I misunderstood, but I think that the stated target blood glucose level of 7.8 to 11.1 mmol/L (140 to 200 mg/dL) includes all patients. When I looked at the references, however, most of them seemed to discuss patients who are receiving nothing by mouth because they are critically ill, are postoperative, or have had recent neurologic events.Most of the time, if a hospitalized patient is eating, we in the medical community almost universally check the preprandial capillary glucose level. If it is 11.1 ...Reference1. Qaseem A, Humphrey LL, Chou R, Snow V, Shekelle P; Clinical Guidelines Committee of the American College of Physicians. Use of intensive insulin therapy for the management of glycemic control in hospitalized patients: a clinical practice guideline from the American College of Physicians. Ann Intern Med. 2011;154:260-7. [PMID: 21320941] LinkGoogle Scholar Author, Article, and Disclosure InformationAuthors: Lynn Bentson, MDAffiliations: From Samaritan Health Physicians, Albany, OR 97321.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoUse of Intensive Insulin Therapy for the Management of Glycemic Control in Hospitalized Patients: A Clinical Practice Guideline From the American College of Physicians Amir Qaseem , Linda L. Humphrey , Roger Chou , Vincenza Snow , and Paul Shekelle , for the Clinical Guidelines Committee of the American College of Physicians* Intensive Insulin Therapy in Hospitalized Patients Anthony P. Furnary Intensive Insulin Therapy in Hospitalized Patients Rajesh Garg Intensive Insulin Therapy in Hospitalized Patients Mary Korytkowski , Etie Moghissi , and Guillermo Umpierrez Intensive Insulin Therapy in Hospitalized Patients Devan Kansagara , Paul Shekelle , and Amir Qaseem Metrics Cited byManagement of Hyperglycemia During the Perioperative PeriodHypoglycemie bij diabetes tijdens ziekenhuisopname: relevant voor klinische uitkomsten? 21 June 2011Volume 154, Issue 12Page: 845KeywordsBloodCapillariesConflicts of interestEatingGlucoseInsulinMouth ePublished: 21 June 2011 Issue Published: 21 June 2011 Copyright & PermissionsCopyright © 2011 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Global microRNA expression profiling uncovers molecular markers for classification and prognosis in aggressive B-cell lymphoma
Authors: Javeed Iqbal, Yulei Shen, Xin Huang, Yanyan Liu, Laura Wake, Cuiling Liu, Karen Deffenbacher, Cynthia M. Lachel, Chao Wang, Joseph Rohr, Shuangping Guo, Lynette M. Smith, George Wright, Sharathkumar Bhagavathi, Karen Dybkaer, Kai Fu, Timothy C. Greiner, Julie M. Vose, Elaine Jaffe, Lisa Rimsza, Andreas Rosenwald, German Ott, Jan Delabie, Elias Campo, Rita M. Braziel, James R. Cook, Raymond R. Tubbs, James O. Armitage, Dennis D. Weisenburger, Louis M. Staudt, Randy D. Gascoyne, Timothy W. McKeithan, Wing C. Chan
Journal: Blood (2014)