tbuckley/cabot-search huggingface dataset:
Genetic Studies on Somatic Mammalian Cells.
Authors: T. T. Puck
Journal: Annals of Internal Medicine (1963)

s1 April 1963Genetic Studies on Somatic Mammalian Cells.T. T. Puck, Ph.D.T. T. Puck, Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-58-4-734_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptRecent developments in tissue culture techniques for mammalian somatic cells have introduced revolutionary new advances in human genetics. Application of these techniques to human chromosomal analysis has established that more than one-half per cent of all human live births are accompanied by gross chromosomal abnormalities. All of these produce deep-seated disease, and many involve mental deficiency. The most common types of aberrations are errors in chromosome number, due to non-disjunction, and errors in chromosomal structure, usually due to chromosome breakage which is followed by an abnormal reunion. Most of the anomalies produced are limited to about 5 of the chromosome... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: T. T. Puck, Ph.D.Affiliations: Denver, Colo. (ML) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1963Volume 58, Issue 4Page: 734-734KeywordsBirthCellsChromosomesGeneticsHuman geneticsIntellectual disabilityTissue cultures ePublished: 1 December 2008 Issue Published: 1 April 1963 PDF downloadLoading ...
<i>Radiante</i>
Authors: Thomas B. Cole
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Family Medicine: An Inside Look
Authors: William C. Gibson
Journal: JAMA (1980)

This book, written by a general practitioner, not only tells it as it is but will intrigue any reader, professional or layman. What a slice of life Dr Livingston describes; not the African jungle of Dr Livingstone, but the "little jungle" of a family physician's office and his community. The case histories are filled with spice, pathos, and surprises. Here is a family physician who, having studied "manipulation" with medically qualified experts, is willing to take on its practitioners and to puncture some balloons. He has some sharp things to say about the universities' preparation of students for general practice—if it can be called preparation. His quotations from great generalists such as Sir James Mackenzie are marvelous. There is an excellent bibliography. For a few evenings of pleasurable reading this engaging little book is excellent fare—witty, down to earth, and constructive.
The Relation of Hospitals and Health Departments in Tomorrow's World
Authors: Charles F. Wilinsky
Journal: New England Journal of Medicine (1954)

ALL who are concerned with the advancement of the public health derive comfort and satisfaction from the increasing interest in the furtherance of closer working relations between hospitals and health departments. Much has been said and a great deal has been written authoritatively on the values of this relation. Almost twenty years ago it was my privilege to serve as chairman of the Committee on Public Health Relations of the American Hospital Association, whose report to the hospitals of America made certain recommendations that hold true and are rich in values today. About five years ago the American Hospital Association . . .
Renal transplantation in the modern immunosuppressive era in Spain: four-year results from a multicenter database focus on post-transplant cardiovascular disease
Authors: Jose M. Morales, Roberto Marcén, Amado Andrés, Miguel González Molina, Domingo del Castillo, Mercedes Cabello, Luis Capdevila, Josep M. Campistol, Federico Oppenheimer, Daniel Serón, Salvador Gil Vernet, Ildefonso Lampreave, Francisco Valdés, Fernando Anaya, Fernando Escuín, Manuel Arias, Luis Pallardó, Jesús Bustamante
Journal: Kidney International (2008)
Secondary Retroperitoneal Fibrosis Due to Polyarteritis Nodosa Evaluated by F-18 FDG PET/CT
Authors: Yoshinori Taniguchi, Yoshitaka Kumon, Toru Kagawa, Yoshio Terada
Journal: Clinical Nuclear Medicine (2011)

A 38-year-old man presented with fever, low back pain, orchialgia, and weight loss for two months. He had recently begun dialysis. Laboratory studies revealed CRP 16.0 mg/dl and sCr 13.08 mg/dl. FDG PET/CT demonstrated uptake in a periaortic mass, medium sized visceral arteries, hydronephrosis. Histological examination of the mass revealed retroperitoneal fibrosis (RPF). 3D-CT angiography showed multiple aneurysms, consistent with polyarteritis nodosa (PAN). RPF secondary to PAN was clinically diagnosed. All the symptoms and abnormal laboratory and FDG PET/CT findings have disappeared after three months of glucocorticoid therapy.
Epidemiology of house-dust mites
Authors: J. Korsgaard
Journal: Allergy (1998)

Available epidemiologic data on the occurrence of house‐dust mites in dwellings demonstrates a clear association between increased indoor air humidity and the increased occurrence of house‐dust mites in house dust. Furthermore, in temperate climates, there is a threshold level of indoor air humidity of 7 g/kg (45% relative humidity at usual indoor air temperatures). Indoor air humidities below this level for extended periods will eradicate house‐dust mites from dwellings. A reduction in inhabitant exposure to house‐dust mites is implemented by reduction of indoor air humidity by controlled mechanical ventilation. Individual ventilation levels are estimated from the actual size of house, number of inhabitants, and average outdoor air humidity in winter. In contrast, more humid areas of the world with average outdoor humidities above 6–7 g/kg in winter will support uniformly large populations of house‐dust mites, and reductions in indoor air humidity will have a comparatively minor effect on the occurrence of house‐dust mites. Present‐day building of energy‐efficient houses with increased sealing of the building envelope, paralleled by a similar renovation of older houses, has increased indoor air humidity and is probably the cause of the almost fourfold increase in the occurrence of house‐dust mites in Danish dwellings.
Boundaries in the Physician-Patient Relationship
Authors: Allen Fay
Journal: JAMA (1995)

<h3>To the Editor.</h3> —Drs Gabbard and Nadelson<sup>1</sup>have performed a valuable service by keeping a sharp focus on the critically important issue of patient exploitation by health care professionals. Despite their many cogent and compelling points, particularly concerning sexual abuse, they have made two significant errors, one in logic and the other factual. The error in logic involves the "slippery slope" argument, which can be summarized as follows. Sexual exploitation of patients by physicians is usually preceded by other behaviors (eg, selfdisclosure); therefore, physicians who engage in such non-sexual "boundary violations" are likely to exploit their patients sexually. The specific factual error has to do with self-disclosure itself: "Even if revealing personal issues to a patient does not lead progressively to more extreme boundary violations, self-disclosure is itself a boundary problem because it is a misuse of the patient to satisfy one's own needs for comfort or sympathy." No
Residual activity of cetrimide and chlorhexidine on Enterococcus faecalis-infected root canals
Authors: Carmen María Ferrer-Luque, María Teresa Arias-Moliz, Matilde Ruíz-Linares, María Elena Martínez García, Pilar Baca
Journal: International Journal of Oral Science (2013)

Effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. The aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown on uniradicular roots for 4 weeks. After root canal preparation, root canals were irrigated with 17% ethylenediaminetetraacetic acid (EDTA) to remove the smear layer. The roots were randomly divided into three experimental groups (n=26) according to the final irrigating solution: Group I, 5 mL 0.2% cetrimide; Group II, 5 mL 0.2% chlorhexidine; and Group III, 5 mL 2% chlorhexidine. Samples were collected for 50 days to denote the presence of bacterial growth. The proportion of ungrown specimens over 50 days was evaluated using the nonparametric Kaplan–Meier survival analysis. Differences among groups were tested using the log-rank test and the level of statistical significance was set at P<0.05. The highest survival value was found with 2% chlorhexidine, showing statistically significant differences from the other two groups. At 50 days, E. faecalis growth was detected in 69.23% specimens in Groups I and II, and in 34.61% specimens of Group III. There were no significant differences between 0.2% cetrimide and 0.2% chlorhexidine. Final irrigation with 2% chlorhexidine showed greater residual activity than 0.2% chlorhexidine and 0.2% cetrimide in root canals infected with E. faecalis. Cleaning root canals with chlorhexidine antiseptic leaves a sustained residual antimicrobial effect, research from Spain shows. Root canal surgery carries a high risk of infection: the bacteria Enterococcus faecalis is commonly found following the procedure. Effective irrigation of root canals during root canal treatment disinfects the area and can provide a barrier to future infection by leaving an antimicrobial residue to attack remaining bacteria. To verify the most effective treatment, Carmen Maria Ferrer-Luque and colleagues at the University of Granada compared three irrigation solutions on root canals infected with E. faecalis. After 50 days, the team found significant bacterial growth on 69% of roots treated with weak solutions (0.2%) of cetrimide and chlorhexidine. A more potent chlorhexidine solution (2%), however, indicated a longer-lasting residual effect, with E. faecalis growth on less than 35% of roots.
Patients With Hemodynamically Tolerated Ventricular Tachycardia Require Implantable Cardioverter–Defibrillators
Authors: David J. Callans
Journal: Circulation (2007)

onventional wisdom has long held that patients with tolerated ventricular tachycardia (VT) in the setting of chronic coronary heart disease are at low risk of arrhythmic death.This logic held that arrhythmia recurrence, although reasonably likely, could be predicted to be well tolerated.As with all truisms, it is good to reexamine this belief periodically.Disease states change; more information becomes available; and the general context of medical care improves.Expectations and capacity to tolerate risk, which are essentially societal rather than medical considerations, continue to evolve.Situations exist such as the present case in which this process of reexamination rather than the results of well-constructed randomized trials will determine the correct course.
Conditional model to study the tissue- and time specific effects of nadph oxidase 2 -derived reactive oxygen species during arthritis
Authors: Outi Sareila, Pia Rantakari, Matti Poutanen, Rikard Holmdahl
Journal: Annals of the Rheumatic Diseases (2012)

<h3>Background and objectives</h3> Neutrophil cytosolic factor 1 (NCF1) is the key regulatory component of the phagocytic NADPH oxidase 2 (NOX2) complex, and mutations in this gene lead to lowered production of reactive oxygen species (ROS). <i>Ncf1</i> has been linked to rheumatoid arthritis, and naturally occurring mutations in this gene have shown to increase arthritis severity in rodents. Interestingly, ROS production protected rodents from autoimmunity which is against the dogma that ROS are solely harmful in inflammation. In this study, the authors aimed at generating targeted <i>Ncf1</i> knock-in mice in which <i>Ncf1</i> expression can be induced in preferred cell type or at a desired time during the disease progression. This enables us to identify the critical cell type and the time window during which the <i>Ncf1</i> expression would protect the mice from arthritis. <h3>Materials and methods</h3> The authors generated the targeted <i>Ncf1</i> knock-in mice on a pure C57BL/6N genetic background. Those mice were compared with B10Q.<i>Ncf1</i><sup><i>m1J</i></sup> mice which have an intronic SNP in their <i>Ncf1</i> gene, leading to lowered ROS production by the NOX2 complex. In the <i>Ncf1</i> knock-in mice <i>Ncf1</i> expression was induced in vivo by Cre or FLP recombinase, and the gene activation was followed by analysing a simultaneously activated EGFP expression, used as a reporter gene. NCF1 protein variants were generated by in vitro mutagenesis and their activity assessed by luminometric ROS measurements. <h3>Results</h3> The targeting silenced the <i>Ncf1</i> gene as intended. The authors observed that, in terms of ROS production, the B10Q <i>Ncf1<sup>m1J</sup></i> mice and the knock-in mice were similar to each other. The observation was supported by the data indicating that the NCF1 protein variants expressed at low levels in the B10Q <i>Ncf1<sup>m1J</sup></i> mice were completely defective in activating the NOX2 complex to produce ROS. After activating the targeted knock-in gene by recombination, using universally expressed Cre or FLP recombinase, the cell specific expression pattern of NCF1 was similar to that of the wild type mice, mostly showing expression in granulocytes and other cells known to make oxidative burst. <h3>Conclusions</h3> The data indicated that the knock-in mice produced do not express functional <i>Ncf1</i>, while the NCF1 expression can be activated by universally expressed Cre of FLP recombinase, inducing a physiological expression pattern of the protein. The model can, thus, be utilised to study the spatiotemporal effects of reactive oxygen species generated by the NOX2 complex.
Sildenafil Failures May Be Due to Inadequate Patient Instructions and Follow-Up: A Study on 100 Non-Responders
Authors: Dimitrios Hatzichristou, Kyriakos Moysidis, Apostolos Apostolidis, Athanasios Bekos, Vasilios Tzortzis, Konstantinos Hatzimouratidis, Evangelos Ioannidis
Journal: European Urology (2005)
Improved Technique in Fracture Treatment
Authors: St. J. D. Buxton
Journal: BMJ (1967)

and enthusiasm of Professor Gustafson in a very readable and instructive volume.It is only after reading this book that one realizes the enormous forensic significance of the teeth.It has been appreciated for a long time that identification by means of dental records is most reliable and valuable, and many examples can be cited in murder cases in this country, notably Buck Ruxton and Dobkin.Decomposition and destruction by fire occurring in aircraft accidents create circumstances when identification by dental records is the only certain method available.
Noncardiac Comorbidities in Heart Failure With Reduced Versus Preserved Ejection Fraction
Authors: Robert J. Mentz, Jacob P. Kelly, Thomas G. von Lueder, Adriaan A. Voors, Carolyn S.P. Lam, Martin R. Cowie, Keld Kjeldsen, Ewa A. Jankowska, Dan Atar, Javed Butler, Mona Fiuzat, Faiez Zannad, Bertram Pitt, Christopher M. O’Connor
Journal: Journal of the American College of Cardiology (2014)
BMA calls on consultants to challenge long hours of work
Authors: L. Eaton
Journal: BMJ (2003)

The BMA is issuing consultants in England with guidelines on how they can challenge their managers over long working hours and claim additional payment for extra time worked, as part of the ongoing campaign to resist proposed changes to their contract.

The association suggests that consultants who regularly work beyond their job plan should ask for a review of their plan, which they should support with a detailed diary showing their NHS work commitment. This should include work done out of hours, emergency work, administrative duties, and teaching and research commitments.

The guidance also suggests that …
Surgeon threatened with libel over remarks made about a breast enhancement cream
Authors: C. Dyer
Journal: BMJ (2010)

A consultant plastic surgeon faces a possible libel action for questioning the effectiveness of a £125 (€146; $200) cream that its makers claim will increase a woman’s breast size.

The comments by Dalia Nield of the London Clinic were quoted by the Daily Mail in an article about Rodial’s Boob Job cream, which the manufacturers say can boost breast size by 2.5 cm.

Mrs Nield was quoted as saying that it was “highly unlikely” that the cream would make breasts bigger and adding that the company had not provided a full analysis of tests on it. If …
CONGENITAL LYMPHANGIECTATIC EDEMA
Authors: HAROLD O. RUH
Journal: JAMA (1925)

One of the most unusual and startling conditions encountered in medical practice is a form of edema of the new-born generally described under the term, congenital elephantiasis. While it is true that this is a proper designation from the standpoint of the main clinical features, nevertheless, the etiologic and pathologic variations and the differences in the character and consistency of the edema call for a descriptive title more accurate.  There are several types of this rare disease, classified according to consistency and surface, and as regards the involvement of vessels, nerves, bones, muscles and fibrous tissue. Since our case conforms rather definitely to the type involving the lymphatics, we shall describe it under the title of congenital lymphangiectatic edema.  REPORT OF CASE  History.  —H. B., a white girl baby, was born, June 10, 1923. Delivery was normal. The birth weight was 6 pounds and 6 ounces (3 kg.). At birth
Response to Letter Regarding Article, “Pulmonary Vascular Disease in the Developing World”
Authors: Ghazwan Butrous, Hossein Ardeschir Ghofrani, Friedrich Grimminger
Journal: Circulation (2009)

We thank Drs Lambertucci and Antunes for their useful and constructive remarks about our article. 1We also appreciate the work of Dr Lambertucci's team in this field, which has increased our knowledge over the last 2 decades.We agree about how the term Katayama syndrome should be used.However, we think that the issue of resolution of chest radiological changes in their article needs some consideration.They said that these patients did not suffer from pulmonary hypertension; however, there was no attempt to investigate that possibility.Furthermore, our current experimental observations suggest that the changes in the pulmonary vasculature after schistosoma infection are far more common but may not always be associated with significant increases in the total vascular resistance.However, this notion needs further investigation.As for the relationship between periportal fibrosis and Symmers pipestem fibrosis, we believe that Drs Lambertucci and Antunes highlighted several important points that need revisiting.First, our knowledge of the incidence and prevalence of pulmonary vascular diseases due to schistosomal infection is far from accurate.Most previously published works (the majority of which came from Brazilian investigations) have been anecdotal observational studies.We have no studies yet from sub-Saharan Africa where 80% of the world's schistosoma infection is present.Second, our main hypothesis is that pulmonary vascular disease is caused by the inflammatory response, most probably to the highly antigenic eggs.Portal hypertension and the development of the collateral circulation probably facilitate the diversion of the eggs to the pulmonary compartment. 2Drs Lambertucci and Antunes state, "Without Symmers fibrosis, portal hypertension related to schistosomiasis has not yet been reported."This statement is correct mainly when the infection is due to Schistosoma mansoni, which is the most prevalent species in Brazil.This species apparently has a higher incidence of pulmonary hypertension than other species, mainly Schistosoma japonicum, 3,4 that affect the intestinal and hepatic circulatory system, because of the different antigenecity of the eggs.Furthermore, pulmonary hypertension has been reported in other species that affect the urinary system, such as Schistosoma hematobium. 5It seems that the pathological changes are not only related to periportal fibrosis but several other factors, including the egg burden, chronicity, reinfection, and coinfections.Genetic factors also predispose certain individuals to develop other conditions depending on gender, nutrition, immunological status, and environment. 6nce again, we thank Drs Lambertucci and Antunes for their excellent and important comments, giving us the opportunity to revisit some controversial issues that are still not fully understood.It is clear that further studies are necessary to better understand the impact of this complex infection on pulmonary vascular diseases.
Clinical and immunologic effects of a rush sublingual immunotherapy to Parietaria species: A double-blind, placebo-controlled trial☆☆☆★
Authors: Giovanni Passalacqua, Monica Albano, Annamaria Riccio, Laura Fregonese, Paola Puccinelli, Silvano Parmiani, Giorgio Walter Canonica
Journal: Journal of Allergy and Clinical Immunology (1999)
Inhibitory influences from arterial baroreceptors on vasopressin release elicited by fastigial stimulation in rats.
Authors: A Del Bo, A F Sved, D J Reis
Journal: Circulation Research (1984)

Electrical stimulation of the fastigial nucleus in anesthetized, paralyzed, and artificially ventilated rats for 10 seconds (50 Hz) induced a stimulus-locked elevation of arterial pressure (the fastigial pressor response) and increased plasma vasopressin. Cervical spinal cord transection abolished the stimulus-locked fastigial pressor response and augmented the vasopressin response to a 10-fold increase (19 +/- 1 to 188 +/- 58 pg/ml, P less than 0.05; n = 8). Grading the pressor elevations occurring during the fastigial nucleus stimulus changed the amounts of vasopressin released in the same animal: acute adrenalectomy and chemosympathectomy by guanethidine reduced the magnitude of the fastigial pressor response and facilitated the vasopressin release to fastigial nucleus stimulation (intact: 52 +/- 11 pg/ml; after adrenalectomy and chemosympathectomy, 254 +/- 73 pg/ml, P less than 0.05, n = 6). Subsequent intravenous administration of a bolus of phenylephrine to increase mean arterial pressure during fastigial nucleus stimulus, as in intact situation, reduced the vasopressin release (47 +/- 9 pg/ml). After sinoaortic denervation plus vagotomy, the fastigial pressor response was preserved; however, vasopressin still increased 11-fold (from 11 +/- 1 to 126 +/- 23 pg/ml, P less than 0.01, n = 8). Vagotomy alone did not affect the vasopressin resting level nor the 4-fold increase in response to fastigial nucleus stimulation. Therefore, stimulus-locked elevations of arterial pressure oppose, by reflex mechanisms mediated through baroreceptors, but do not prevent the release of vasopressin elicited by stimulation of the fastigial nucleus.