tbuckley/cabot-search huggingface dataset:
Upregulation of pro-inflammatory cytokines in the intercostal muscles of COPD patients
Authors: C. Casadevall, C. Coronell, A. L. Ramírez-Sarmiento, J. Martínez-Llorens, E. Barreiro, M. Orozco-Levi, J. Gea
Journal: European Respiratory Journal (2007)

Muscle dysfunction is a characteristic feature of chronic obstructive pulmonary disease (COPD). Recent studies suggest that cytokines may operate as local regulators of both muscle function and regeneration. The aim of the present study was to characterise the expression of different cytokines in the external intercostal muscle of COPD. Muscle biopsies were obtained from 25 stable COPD patients and eight healthy controls. Local tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, -6 and -10 expressions (real-time PCR and ELISA), sarcolemmal damage (immunohistochemistry), and the transcript levels of CD18 were assessed. Muscle TNF-alpha and IL-6 transcripts were significantly higher in COPD patients compared with controls, and IL-1beta and sarcolemmal damage showed a strong tendency in the same direction. Similar results were observed at protein level. The CD18 panleukocyte marker was similar in COPD and controls. Respiratory muscle function was impaired in COPD patients and it correlated to both the severity of lung function impairment and TNF-alpha muscle expression. Chronic obstructive pulmonary disease is associated with the upregulation of pro-inflammatory cytokines in the intercostal muscles. This phenomenon might be involved in respiratory muscle dysfunction.
Simultaneous occurrence of mycosis fungoides and hodgkin disease: Clinical and histologic correlations in three cases with ultrastructural studies in two
Authors: Katherine A. Hawkins, Roger Schinella, Melvin Schwartz, David Ramsey, Arthur H. Weintraub, Robert Silber, Edward L. Amorosi
Journal: American Journal of Hematology (1983)

Abstract We present three patients who manifested both Hodgkin disease and mycosis fungoides. Ages ranged from 39 to 66 and two were male. Skin lesions were present from 3 to 40 years before the diagnosis of Hodgkin disease. In all cases, mycosis fungoides was confirmed histologically by skin biopsy; the clinical course of the mycosis fungoides was indolent in all cases. Hodgkin disease was confirmed histologically in three, and confirmed by electron microscopy in two. All three patients responded to appropriate treatment for Hodgkin disease and are alive and well at the present time.
Flightless I is a key regulator of the fibroproliferative process in hypertrophic scarring and a target for a novel antiscarring therapy
Authors: A.M. Cameron, C.T. Turner, D.H. Adams, J.E. Jackson, E. Melville, R.M. Arkell, P.J. Anderson, A.J. Cowin
Journal: British Journal of Dermatology (2015)

Journal Article Flightless I is a key regulator of the fibroproliferative process in hypertrophic scarring and a target for a novel antiscarring therapy Get access A.M. Cameron, A.M. Cameron Regenerative Medicine Future Industries Institute University of South Australia Mawson Lakes SA 5095 AustraliaDiscipline of Surgery School of Medicine Faculty of Health Sciences The University of Adelaide Adelaide SA Australia Search for other works by this author on: Oxford Academic Google Scholar C.T. Turner, C.T. Turner Regenerative Medicine Future Industries Institute University of South Australia Mawson Lakes SA 5095 Australia Search for other works by this author on: Oxford Academic Google Scholar D.H. Adams, D.H. Adams Regenerative Medicine Future Industries Institute University of South Australia Mawson Lakes SA 5095 Australia Search for other works by this author on: Oxford Academic Google Scholar J.E. Jackson, J.E. Jackson Regenerative Medicine Future Industries Institute University of South Australia Mawson Lakes SA 5095 Australia Search for other works by this author on: Oxford Academic Google Scholar E. Melville, E. Melville Regenerative Medicine Future Industries Institute University of South Australia Mawson Lakes SA 5095 Australia Search for other works by this author on: Oxford Academic Google Scholar R.M. Arkell, R.M. Arkell Research School of Biology College of Medicine, Biology and Environment Australian National University Acton ACT 2601 Australia Search for other works by this author on: Oxford Academic Google Scholar P.J. Anderson, P.J. Anderson Discipline of Paediatrics School of Medicine Faculty of Health Sciences The University of Adelaide Adelaide SA Australia Search for other works by this author on: Oxford Academic Google Scholar A.J. Cowin A.J. Cowin Regenerative Medicine Future Industries Institute University of South Australia Mawson Lakes SA 5095 Australia Correspondence Allison Cowin. E‐mail: allison.cowin@unisa.edu.au Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 174, Issue 4, 1 April 2016, Pages 786–794, https://doi.org/10.1111/bjd.14263 Published: 01 April 2016
Rat lymphoid cell lines producing human T cell leukemia virus. II. Constitutive expression of rat interleukin 2 receptor.
Authors: J Yodoi, M Okada, Y Tagaya, K Teshigawara, K Fukui, N Ishida, K Ikuta, M Maeda, T Honjo, H Osawa
Journal: The Journal of Experimental Medicine (1985)

Three rat lymphoid cell lines (TARS-1, TARL-2, and TART-1) (12) transformed by human T cell leukemia/lymphoma virus I (HTLV-I) had rearrangement of the beta chain gene of the T cell antigen receptor, and had integrated proviral DNA from HTLV-I in their genomes. As is the case with adult T cell leukemia (ATL)-derived human T cell lines transformed by HTLV-I, these rat cell lines unequivocally expressed interleukin 2 (IL-2) receptor, as determined by radiolabeled IL-2 binding. By Scatchard plot analysis, one of the cell lines, TART-1, proved to have high affinity receptors (Ka = 1.3 X 10(11)/M and 8.8 X 10(9)/M). Rat IL-2 receptor, not human IL-2 receptor, was expressed on HTLV+ rat cell lines, as demonstrated by the fact that they expressed antigens reactive with monoclonal antibodies (ART-18) against rat IL-2 receptor, but not with anti-Tac antibodies. The collective evidence indicates that the endogenous IL-2 receptor gene is activated in human and rat lymphoid cell lines with HTLV-I production. The mechanism of abnormal IL-2 receptor expression in HTLV infection is discussed.
The relationship of dyspnoea to function and quality of life in systemic sclerosis
Authors: M Baron, E Sutton, M Hudson, B Thombs, J Markland, J Pope, D Robinson, N Jones, P Docherty, M Abu-Hakima, S LeClercq, D Smith, J-P Mathieu
Journal: Annals of the Rheumatic Diseases (2007)

<h3>Aim:</h3> Up to 50% of patients with systemic sclerosis (SSc) have complaints of dyspnoea. We evaluated the independent contributions of dyspnoea to function and health related quality of life (HRQoL) in SSc and also assessed the contributions of pulmonary hypertension, measured by the pulmonary artery systolic pressure (PASP), and interstitial lung disease, measured by the forced vital capacity (FVC), to dyspnoea. <h3>Methods:</h3> We assessed dyspnoea, PASP, FVC, function and HRQoL in a cohort of unselected patients with SSc. Multiple linear regression was used to assess the independent contributions of dyspnoea, PASP and FVC to function and HRQoL, after controlling for possible confounding variables. <h3>Results:</h3> A total of 194 patients with mean disease duration of 11.6 years were studied. Dyspnoea was a significant independent predictor of function and HRQoL. A model including age, gender, disease duration, disease severity and dyspnoea explained 33.3%, 10.6%, 39.2% and 29.4% of the variance of the Stanford Health Assessment Questionnaire, the Short-Form 36 (SF-36) mental component summary score, the SF-36 physical component summary score and the World Health Organization Disability Assessment Schedule II. PASP and FVC were significant independent predictors of dyspnoea but only 21.9% of the variance in dyspnoea was explained by age, gender, disease duration, FVC and PASP. The FVC was a significant independent predictor of function and HRQoL. <h3>Conclusion:</h3> In an unselected population of SSc patients, dyspnoea is a very important contributor to function and HRQoL. Interstitial lung disease, as measured by the FVC, contributes significantly to the sense of dyspnoea, function and HRQoL in SSc. Pulmonary hypertension, assessed echocardiographically by the PASP, predicts the degree of dyspnoea but not function and HRQoL in SSc.
An Epstein-Barr virus–encoded microRNA targets PUMA to promote host cell survival
Authors: Elizabeth Yee-Wai Choy, Kam-Leung Siu, Kin-Hang Kok, Raymond Wai-Ming Lung, Chi Man Tsang, Ka-Fai To, Dora Lai-Wan Kwong, Sai Wah Tsao, Dong-Yan Jin
Journal: The Journal of Experimental Medicine (2008)

Epstein-Barr virus (EBV) is a herpesvirus associated with nasopharyngeal carcinoma (NPC), gastric carcinoma (GC), and other malignancies. EBV is the first human virus found to express microRNAs (miRNAs), the functions of which remain largely unknown. We report on the regulation of a cellular protein named p53 up-regulated modulator of apoptosis (PUMA) by an EBV miRNA known as miR-BART5, which is abundantly expressed in NPC and EBV-GC cells. Modulation of PUMA expression by miR-BART5 and anti–miR-BART5 oligonucleotide was demonstrated in EBV-positive cells. In addition, PUMA was found to be significantly underexpressed in ∼60% of human NPC tissues. Although expression of miR-BART5 rendered NPC and EBV-GC cells less sensitive to proapoptotic agents, apoptosis can be triggered by depleting miR-BART5 or inducing the expression of PUMA. Collectively, our findings suggest that EBV encodes an miRNA to facilitate the establishment of latent infection by promoting host cell survival.
Health outcomes of youth development programme in England: prospective matched comparison study
Authors: M. Wiggins, C. Bonell, M. Sawtell, H. Austerberry, H. Burchett, E. Allen, V. Strange
Journal: BMJ (2009)

To evaluate the effectiveness of youth development in reducing teenage pregnancy, substance use, and other outcomes.Prospective matched comparison study.54 youth service sites in England.Young people (n=2724) aged 13-15 years at baseline deemed by professionals as at risk of teenage pregnancy, substance misuse, or school exclusion or to be vulnerable.Intensive, multicomponent youth development programme including sex and drugs education (Young People's Development Programme) versus standard youth provision.Various, including pregnancy, weekly cannabis use, and monthly drunkenness at 18 months.Young women in the intervention group more commonly reported pregnancy than did those in the comparison group (16% v 6%; adjusted odds ratio 3.55, 95% confidence interval 1.32 to 9.50). Young women in the intervention group also more commonly reported early heterosexual experience (58% v 33%; adjusted odds ratio 2.53, 1.09 to 5.92) and expectation of teenage parenthood (34% v 24%; 1.61, 1.07 to 2.43).No evidence was found that the intervention was effective in delaying heterosexual experience or reducing pregnancies, drunkenness, or cannabis use. Some results suggested an adverse effect. Although methodological limitations may at least partly explain these findings, any further implementation of such interventions in the UK should be only within randomised trials.
Regarding Review of Psychopathology in Children and Adolescents Who Are Deaf or Hard of Hearing
Authors: Rachel St. John
Journal: JAMA Pediatrics (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Sirolimus Is Associated with New-Onset Diabetes in Kidney Transplant Recipients
Authors: Olwyn Johnston, Caren L. Rose, Angela C. Webster, John S. Gill
Journal: Journal of the American Society of Nephrology (2008)

New-onset diabetes (NOD) is associated with transplant failure. A few single-center studies have suggested that sirolimus is associated with NOD, but this is not well established. With the use of data from the United States Renal Data System, this study evaluated the association between sirolimus use at the time of transplantation and NOD among 20,124 adult recipients of a first kidney transplant without diabetes. Compared with patients treated with cyclosporine and either mycophenolate mofetil orazathioprine, sirolimus-treated patients were at increased risk for NOD, whether it was used in combination with cyclosporine (adjusted hazard ratio [HR] 1.61; 95% confidence interval [CI] 1.36 to 1.90),tacrolimus (adjusted HR 1.66; 95% CI 1.42 to 1.93), or an antimetabolite (mycophenolate mofetil orazathioprine; adjusted HR 1.36; 95% CI 1.09 to 1.69). Similar results were obtained in a subgroup analysis that included the 16,861 patients who did not have their immunosuppressive regimen changed throughout the first posttransplantation year. In conclusion, sirolimus is independently associated with NOD. Given the negative impact of NOD on posttransplantation outcomes, these findings should be confirmed in prospective studies or in meta-analyses of existing trials that involved sirolimus.
Regulation of Breast Cancer Stem Cell Activity by Signaling through the Notch4 Receptor
Authors: Hannah Harrison, Gillian Farnie, Sacha J. Howell, Rebecca E. Rock, Spyros Stylianou, Keith R. Brennan, Nigel J. Bundred, Robert B. Clarke
Journal: Cancer Research (2010)

Notch receptor signaling pathways play an important role not only in normal breast development but also in breast cancer development and progression. We assessed the role of Notch receptors in stem cell activity in breast cancer cell lines and nine primary human tumor samples. Stem cells were enriched by selection of anoikis-resistant cells or cells expressing the membrane phenotype ESA(+)/CD44(+)/CD24(low). Using these breast cancer stem cell populations, we compared the activation status of Notch receptors with the status in luminally differentiated cells, and we evaluated the consequences of pathway inhibition in vitro and in vivo. We found that Notch4 signaling activity was 8-fold higher in stem cell-enriched cell populations compared with differentiated cells, whereas Notch1 signaling activity was 4-fold lower in the stem cell-enriched cell populations. Pharmacologic or genetic inhibition of Notch1 or Notch4 reduced stem cell activity in vitro and reduced tumor formation in vivo, but Notch4 inhibition produced a more robust effect with a complete inhibition of tumor initiation observed. Our findings suggest that Notch4-targeted therapies will be more effective than targeting Notch1 in suppressing breast cancer recurrence, as it is initiated by breast cancer stem cells.
Text-mining assisted regulatory annotation
Authors: Stein Aerts, Maximilian Haeussler, Steven van Vooren, Obi L Griffith, Paco Hulpiau, Steven JM Jones, Stephen B Montgomery, Casey M Bergman, The Open Regulatory Annotation Consortium
Journal: Genome biology (2008)

Decoding transcriptional regulatory networks and the genomic cis-regulatory logic implemented in their control nodes is a fundamental challenge in genome biology. High-throughput computational and experimental analyses of regulatory networks and sequences rely heavily on positive control data from prior small-scale experiments, but the vast majority of previously discovered regulatory data remains locked in the biomedical literature. We develop text-mining strategies to identify relevant publications and extract sequence information to assist the regulatory annotation process. Using a vector space model to identify Medline abstracts from papers likely to have high cis-regulatory content, we demonstrate that document relevance ranking can assist the curation of transcriptional regulatory networks and estimate that, minimally, 30,000 papers harbor unannotated cis-regulatory data. In addition, we show that DNA sequences can be extracted from primary text with high cis-regulatory content and mapped to genome sequences as a means of identifying the location, organism and target gene information that is critical to the cis-regulatory annotation process. Our results demonstrate that text-mining technologies can be successfully integrated with genome annotation systems, thereby increasing the availability of annotated cis-regulatory data needed to catalyze advances in the field of gene regulation.
Long-Term Results From a Randomized Phase II Trial of Standard- Versus Higher-Dose Imatinib Mesylate for Patients With Unresectable or Metastatic Gastrointestinal Stromal Tumors Expressing <i>KIT</i>
Authors: Charles D. Blanke, George D. Demetri, Margaret von Mehren, Michael C. Heinrich, Burton Eisenberg, Jonathan A. Fletcher, Christopher L. Corless, Christopher D.M. Fletcher, Peter J. Roberts, Daniela Heinz, Elisabeth Wehre, Zariana Nikolova, Heikki Joensuu
Journal: Journal of Clinical Oncology (2008)

The outcome of patients diagnosed with advanced gastrointestinal stromal tumor (GIST) and treated long-term with imatinib mesylate is unknown. A previous report of a randomized phase II trial of imatinib mesylate in patients with incurable GIST detailed high response rates at both the 400 and the 600 mg/d dose levels. We conducted a long-term analysis of patients treated on the trial, including patients followed during an extension phase, to evaluate survival, patterns of failure, and potential prognostic factors, including tumor mutational status.Patients with advanced GIST were enrolled onto an open-label, multicenter trial and were randomly assigned (1:1) to receive imatinib 400 versus 600 mg/d. Data were prospectively collected on KIT mutational status, total tumor area, and other potential prognostic factors. Patients were followed for a median of 63 months.One hundred forty-seven patients were enrolled: 73 were in arm A (imatinib 400 mg/d), and 74 were in arm B (imatinib 600 mg/d). Response rates, median progression-free survival, and median overall survival were essentially identical on both arms, and median survival was 57 months for all patients. Forty-one patients overall (28%) remained on the drug long-term. Female sex, the presence of an exon 11 mutation, and normal albumin and neutrophil levels were independently associated with better survival.Nearly 50% of patients with advanced GIST who were treated with imatinib mesylate survived for more than 5 years, regardless of a 400 or 600 mg/d starting dose.
A Little pRB Can Lead to Big Problems
Authors: Philip W. Hinds
Journal: Cancer Discovery (2014)

Abstract Summary: Germline deletion of RB1, the gene encoding the retinoblastoma tumor-suppressor protein pRB, predisposes to eye tumor formation upon loss of the remaining wild-type allele. Many functions affecting cell-cycle control, cell-cycle exit, and numerous other processes involved in the transformed phenotype have been ascribed to pRB, and deregulation of these processes is generally thought to result from complete loss of pRB in both hereditary and sporadic tumors in multiple tissues. Loss of just one allele of RB1 is now shown to lead to replication stress and aneuploidy in both mouse and human cells, and the mechanism through which this haploinsufficient phenotype is achieved may open up new opportunities for interceding both in tumor initiation and in treatment of extant tumors. Cancer Discov; 4(7); 764–5. ©2014 AACR. See related article by Coschi et al., p. 840
EULAR Standing Committee of Investigative Rheumatology: report of activities 2002–3
Authors: T W J Huizinga
Journal: Annals of the Rheumatic Diseases (2003)

In the genomics workshop held at the EWRR in Marseille on the 27 February lively discussions were held on the progress of genomics research. Two general overviews were presented by Tom Huizinga (Department of Rheumatology, Leiden University Medical Centre, The Netherlands) and Gerd Burmester (Department of Rheumatology and Clinical Immunology, Charite University Clinic, Berlin, Germany), which served as a starting point for the discussions. …
Compartmentalization of adriamycin and daunomycin in cultured chick cardiac myocytes. Effects on synthesis of contractile and cytoplasmic proteins.
Authors: W Lewis, M Galizi, S Puszkin
Journal: Circulation Research (1983)

The affinity of two anthracycline antineoplastics [( 14C]adriamycin and [14C]daunomycin) for cultured embryonic chick heart cells was determined by measuring their uptake, compartmentalization into subcellular fractions, and effects on the synthesis of cytoplasmic and cytoskeletal proteins. Both drugs, at micromolar concentrations, were readily uptaken by myocytes and found to be concentrated in a light-buoyant-density fraction containing no lysosomes. Nuclear 14C drug content accounted for 20-25% of the drug incorporated. Binding of adriamycin was saturable within 90 minutes of drug exposure, and the uptake of [14C]adriamycin was inhibited 50% by verapamil and adenosine triphosphate. Uptake of [14C]daunomycin was not influenced by these compounds. Cytosolic and contractile protein synthesis measured by [35S]methionine incorporation into proteins was blocked 70% overall in each fraction after 6 hours of incubation with 2 microM adriamycin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography and quantitative densitometry, revealed that actin synthesis was the least affected of the major proteins. Cardiac myocytes incubated for short periods of time with 2 microM adriamycin revealed subtle cytoplasmic changes in their organelles with the appearance of clear zones of cytoplasm containing short unorganized microfilaments. The deleterious effects of anthracyclines in heart cells are manifested early by rapid drug incorporation into myocytes and inhibition of cytoplasmic protein synthesis.