tbuckley/cabot-search huggingface dataset:
Adenoviral SERCA1a gene transfer to adult rat ventricular myocytes induces physiological changes in calcium handling
Authors: Nathalie Chossat, Frank Griscelli, Philippe Jourdon, Damien Logeart, Thierry Ragot, Michèle Heimburger, Michel Perricaudet, Anne-Marie Lompré, Stéphane Hatem, Jean-Jacques Mercadier
Journal: Cardiovascular Research (2001)

Objective: We examined the functional consequences of expressing adult rabbit fast skeletal sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA1a) in isolated adult rat ventricular myocytes. Methods: Myocytes were infected with a recombinant adenovirus harboring SERCA1a. Then 2 days after myocyte infection, protein expression was estimated using Western blot and SDS–PAGE analysis. We also measured the ATP-dependent oxalate-facilitated Ca2+ uptake of myocyte homogenates and monitored Ca2+ transient in myocytes loaded with the Ca2+ dye, indo-1. Results: SERCA1a gene expression resulted in a 36% increase in the total SERCA protein level in infected myocytes compared to controls (P<0.01), while SERCA2 and phospholamban levels did not change. This increase was associated with a 42% rise in SR Ca2+ uptake (P<0.01), while τ (the time constant of Ca2+ transient decay), and the time to peak fell by 32% (P<0.01) and 38% (P<0.001), respectively. Increasing the frequency of stimulation from 0.2 to 2 Hz decreased τ in both cell types (P<0.01). However, the decrease was much smaller in infected (P<0.01) than in uninfected cells (P<0.001). Isoproterenol (1 μM) further decreased τ in infected myocytes by 23% (P<0.05). In these cells, the diastolic [Ca2+]i decreased by 50% (P<0.05) while the systolic [Ca2+]i increased by 19% (P<0.05). No difference was found in the speed of SR Ca2+ reloading after caffeine washout between the two cell types. Conclusion: Adenovirus-mediated SERCA1a gene transfer to adult rat ventricular myocytes enhances SR Ca2+ handling to a degree similar to that observed following physiological stimulation.
Splenic Visualization in a Patient with Glucose-6-Phosphate Dehydrogenase Deficiency
Authors: CARL M. LIEBERMAN, DENNIS L. HEMINGWAY
Journal: Clinical Nuclear Medicine (1979)

A case of splenic visualization of 99mTc-methylene diphosphonate in a patient with G-6PD deficiency who did not show radiographic evidence of splenic calcification is presented and discussed.
Comparison of hypoplastic myelodysplastic syndrome (MDS) with normo-/hypercellular MDS by International Prognostic Scoring System, cytogenetic and genetic studies
Authors: T-C Huang, B-S Ko, J-L Tang, C Hsu, C-Y Chen, W Tsay, S-Y Huang, M Yao, Y-C Chen, M-C Shen, C-H Wang, H-F Tien
Journal: Leukemia (2007)
PRE-HOSPITAL REDUCED-DOSE FIBRINOLYTIC THERAPY FOLLOWED BY EMERGENT PERCUTANEOUS INTERVENTION REDUCES INFARCT SIZE AND LIMITS MICROVASCULAR OBSTRUCTION IN PATIENTS TREATED WITHIN SIX HOURS OF STEMI ONSET WITHOUT A BLEEDING PENALTY
Authors: Troy Weirick, Colin Barker, John LeDoux, Daniel Maland, Eugene Stautberg, Ali Denktas, Stephano Sdringola, Vernon Anderson, Catalin Loghin, Jamie McCarthy, David Persse, Mary Vooletich, Richard Smalling
Journal: Journal of the American College of Cardiology (2010)
Randomised Controlled Trial of a Collaborative Care Model with Psychiatric Consultation for Persistent Medically Unexplained Symptoms in General Practice
Authors: Christina M. van der Feltz-Cornelis, Patricia van Oppen, Herman J. Adèr, Richard van Dyck
Journal: Psychotherapy and Psychosomatics (2006)

&lt;i&gt;Background:&lt;/i&gt; Patients with persistent medically unexplained symptoms often exhibit general dysfunction and psychiatric comorbidity and frequently resist psychiatric referral. The aim of this study was to evaluate the efficacy of a collaborative care model including training for general practitioners (GPs) and a psychiatric consultation model for patients with persistent medically unexplained symptoms in general practice. &lt;i&gt;Method:&lt;/i&gt; Randomised controlled trial. Cluster randomisation at GP practices and multilevel analysis were performed. A total of 81 patients from 36 general practices completed the study. A collaborative care model of training and psychiatric consultation in general practice in the presence of the GP was compared with training plus care as usual by the GP. Outcome assessment on the patients’ well-being, functioning and utilisation of health care services was performed 6 weeks and 6 months later. &lt;i&gt;Results:&lt;/i&gt; All the patients had somatoform disorders (Whitely Index 7.46), and 86% had comorbid psychiatric disorders. In the intervention group, the severity of the main medically unexplained symptoms decreased by 58%. The patients’ social functioning improved. The utilization of health care was lower than in the care as usual group. &lt;i&gt;Conclusions:&lt;/i&gt; A collaborative care model combining training with psychiatric consultation in the general practice setting is an effective intervention in the treatment of persistent medically unexplained symptoms. Anxiety and depressive disorders are highly comorbid in this group. The findings warrant a larger study.
Timing of Glycoprotein IIb/IIIa Inhibitors in Acute Coronary Syndromes—Reply
Authors: Gregg W. Stone, for the ACUITY Investigators
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
TROPONIN T TESTING IN THE PREHOSPITAL DIAGNOSIS AND TRIAGE OF PATIENTS WITH SUSPECTED HEART ATTACK
Authors: Jacob T. Sorensen, Christian J. Terkelsen, Carsten Steengaard, Jens F. Lassen, Sven Trautner, Erika F. Christensen, Torsten T. Nielsen, Hans Erik Botker, Henning R. Andersen, Kristian Thygesen
Journal: Journal of the American College of Cardiology (2011)
p53 in Prostate Cancer: Frequent Expressed Transition Mutations
Authors: S.-G. Chi, R. W. deVere White, F. J. Meyers, D. B. Siders, F. Lee, P. H. Gumerlock
Journal: JNCI Journal of the National Cancer Institute (1994)

Carcinoma of the prostate is the second most common cause of cancer deaths in men. Little is known about the pathogenesis of this disease and the molecular genetic events that contribute to its development. Molecular studies have begun to reveal biologic characteristics of this disease, notably, the loss of genetic material as determined by studies of restriction fragment length polymorphism, oncogene activation, and production and response to growth factors.Our goal was to characterize p53 gene mutations in human carcinoma of the prostate and to analyze base-pair changes within the coding regions of p53 mRNA (exons 4 through 11).Forty-four prostate tissue specimens and four metastatic lesions were obtained from 48 prostate carcinoma patients who had surgical resection. RNA was either immediately extracted or the specimens were snap-frozen in liquid N2 and stored at -70 degrees C until used. Total RNA was extracted from tumor specimens. Expression of p53 was analyzed by polymerase chain reaction (PCR) analysis of mRNA (RNA/PCR). Following confirmation of the RNA/PCR products by Southern blotting, quantitation of message levels was performed by laser densitometry. Absolute area integrations of the curves representing each tissue were then compared after adjustment for the housekeeping gene c-N-ras. Two overlapping regions (exons 4-6 and 6-11) were examined by a nonisotopic PCR single-strand conformation polymorphism (SSCP) analysis system. All specimens displaying SSCP abnormalities were sequenced in both directions to confirm the findings.Of the 48 prostate specimens, three (6%) (two primary and one metastatic) displayed nearly undetectable expression of p53 mRNA (samples PS-70, L113, and PS-95) and 17 (35%) of 48 expressed mutant p53 mRNA encoding amino acid substitutions within exons 4-11 (14 of 17) and/or deletions within the p53 transcripts (three of 17). Overall, the frequency of p53 gene abnormalities that would result in altered protein expression was 20 (42%) of 48 in the tissue samples from prostate carcinoma patients. Nucleotide base-pair transitions of A-->G or T-->C were the most frequent.p53 mutations are common in prostate cancer. The patterns of p53 gene mutations are dramatically different from data obtained on other cancers and indicate the possible involvement of a carcinogenic agent(s).Further studies are required to determine the biologic role of p53 gene alterations in the development and progression of this disease and to determine whether p53 mutations can be useful as prognostic markers or for the selection of better treatments for prostate cancer patients.
Abstract 4113: Role of GP88 (Progranulin) in the proliferation and survival of non small cell lung carcinoma
Authors: Guanjun Xia, Sayuri Kamimura, Binbin Yue, Jianping Dong, Ginette Serrero
Journal: Cancer Research (2010)

Abstract In 2009, 219,000 patients were diagnosed with lung cancer in the US and 159,000 died of the disease. It has been widely reported that results of standard treatment in lung cancer are poor except for the most localized cancers, thereby emphasizing the need to discover novel targets of lung cancer for the development of novel therapies and diagnosis. In the present study, we investigated the role of the autocrine growth and survival factor GP88. GP88 (also known as Progranulin) is an 88kDa glycoprotein that has been shown to play a critical role in the proliferation and survival of breast cancer. Pathological studies with breast cancer cases in correlation with clinical outcome data have also shown that GP88 expression was associated in invasive ductal carcinoma with increased risk of recurrence. In the present study, we examined the role of GP88 in non small cell lung carcinoma (NSCLC). Examination of GP88 protein expression by immunohistochemistry using Oncostain 88 kit was carried out with formalin fixed paraffin embedded sections of lung cancer cases. Results showed that non small cell lung carcinoma, adenocarcinoma and squamous carcinoma stained positive for GP88 whereas small cell carcinoma sections were mostly negative. In addition, normal lung tissues did not express GP88. Based on this observation, expression and action of GP88 in NSCLC was examined using representative cell lines. We show here that GP88 stimulated proliferation and survival of A549 and H1299 cell lines. GP88 expression was upregulated in cells that were made resistant to cytotoxic drugs such as taxol and velcade. Inhibition of GP88 expression by SiRNA or GP88 action by treating the cells with AG1, an anti-GP88 monoclonal antibody resulted in an inhibition of cell migration and proliferation of these drug resistant cells and increased response to cytotoxic drugs. Based on these results, studies were carried out to examine the effect of AG1 antibody on migration and tumor formation of A549 and H1299 cells in vitro and in vivo. Our data that will be presented here point out that GP88 is a novel therapeutic target for NSCLC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4113.
Divergent Nitric Oxide Bioavailability in Men and Women With Sickle Cell Disease
Authors: Mark T. Gladwin, Alan N. Schechter, Frederick P. Ognibene, Wynona A. Coles, Christopher D. Reiter, William H. Schenke, Gyorgy Csako, Myron A. Waclawiw, Julio A. Panza, Richard O. Cannon
Journal: Circulation (2003)

Background— Although reduced endothelial nitric oxide (NO) bioavailability has been demonstrated in arteriosclerotic vascular disease, the integrity of this system in sickle cell disease remains uncertain. Methods and Results— We measured forearm blood flow in 21 patients with sickle cell disease (hemoglobin SS genotype) and 18 black control subjects before and after intra-arterial infusions of acetylcholine, nitroprusside, and the NO synthase inhibitor NG-monomethyl-L-arginine (L-NMMA). Endothelium-dependent vasodilation, measured by the percent increase in flow induced by acetylcholine infusion, was significantly greater than in controls (252±37% for patients versus 134±24% for controls; P &lt;0.0001). However, there was a large sex difference in blood flow responses between female and male patients (340±46% versus 173±41%; P =0.035). Similarly, basal NO bioactivity, as measured by the percent decrease in flow induced by L-NMMA, was depressed in male compared with female patients (−17±5% versus −34±4%; P =0.01), as was the response to nitroprusside (86±21% versus 171±22%; P =0.008). L-NMMA reduced the blood flow response to acetylcholine in women, but not in men. Sex differences in vascular cell adhesion molecule-1 were appreciated, with significant correlations between levels of soluble vascular cell adhesion molecule-1 and blood flow responses to L-NMMA and nitroprusside ( r =0.53, P =0.004 and r =−0.66, P &lt;0.001, respectively). Conclusions— NO bioavailability and NO responsiveness are greater in women than in men with sickle cell disease and determines adhesion molecule expression. Endothelium-dependent blood flows are largely non-NO mediated in male patients. These results provide a possible mechanism for reported sex differences in sickle cell disease morbidity and mortality and provide a basis for novel pharmacological interventions.
Psychiatrists hit back at home secretary
Authors: J. Warden
Journal: BMJ (1998)

The British government clashed with psychiatrists last week over how to deal with people with psychopathic disorders. The disagreement followed criticism of the psychiatric profession by the home secretary, Jack Straw, after the conviction for murder of Michael Stone, who killed a mother and her young daughter in a brutal attack in Kent and who at his trial was described as having been under psychiatric treatment for a personality disorder.

Mr Straw attacked psychiatrists for a reluctance to treat unstable offenders, but …
Netter’s Neurology
Authors: Andreas R. Luft
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Expression of lectin-like LDL receptor (LOX-1) in smooth muscle cells after balloon injury
Authors: Hideyuki Eto, Msaaki Miyata, Noriaki Kume, Manabu Minami, Koji Orthara, Sadatoshi Biro, Shinichi Minagoe, Toru Kita, Chuwa Tei
Journal: Journal of the American College of Cardiology (2002)
No Effect of Adding Brief Dynamic Therapy to Pharmacotherapy in the Treatment of Obsessive-Compulsive Disorder with Concurrent Major Depression
Authors: Giuseppe Maina, Gianluca Rosso, Sylvia Rigardetto, Simone Chiadò Piat, Filippo Bogetto
Journal: Psychotherapy and Psychosomatics (2010)

Until now no studies have investigated the benefits of adding brief dynamic therapy (BDT) to medication in obsessive-compulsive disorder (OCD), while a number of recent investigations have demonstrated the efficacy of supplemental BDT among patients with major depressive disorders (MDD). The objective of the present study was to explore the efficacy of BDT combined with pharmacotherapy in comparison with pharmacotherapy alone in the treatment of OCD with concurrent MDD.A 12-month randomized clinical trial compared a standard selective serotonin reuptake inhibitor treatment with (n = 27) or without (n = 30) supplemental BDT in patients with OCD and concurrent MDD. Supplemental BDT was added during the first 16-week trial; all patients continued to be treated with only pharmacotherapy in the following continuation phase. The primary efficacy assessments were the Yale-Brown Obsessive Compulsive Scale and the 17-item Hamilton Rating Scale for Depression; the secondary efficacy measures included the Clinical Global Impression scale and the Global Assessment of Functioning. The data analysis was conducted on the 'intent-to-treat (ITT) efficacy patient sample'.Fifty patients completed the study. No difference between the 2 treatment groups was found at any point by any assessment method in the ITT study sample.Supplemental BDT in the treatment of patients with OCD with concurrent MDD who are receiving effective medication has no significant clinical effect on both obsessive and depressive symptoms.
Uncertainty beyond sampling error
Authors: D. G. Altman, J. M. Bland
Journal: BMJ (2014)

<h3>Abstract</h3> The genomes of humans outside Africa originated almost entirely from a single migration out ∼50,000-60,000 years ago<sup>1,2</sup>, followed closely by mixture with Neanderthals contributing ∼2% to all non-Africans<sup>3,4</sup>. However, the details of this initial migration remain poorly-understood because no ancient DNA analyses are available from this key time period, and present-day autosomal data are uninformative due to subsequent population movements/reshaping<sup>5</sup>. One locus, however, does retain extensive information from this early period: the Y-chromosome, where a detailed calibrated phylogeny has been constructed<sup>6</sup>. Three present-day Y lineages were carried by the initial migration: the rare haplogroup D, the moderately rare C, and the very common FT lineage which now dominates most non-African populations<sup>6,7</sup>. We show that phylogenetic analyses of haplogroup C, D and FT sequences, including very rare deep-rooting lineages, together with phylogeographic analyses of ancient and present-day non-African Y-chromosomes, all point to East/South-east Asia as the origin 50,000-55,000 years ago of all known non-African male lineages (apart from recent migrants). This implies that the initial Y lineages in populations between Africa and eastern Asia have been entirely replaced by lineages from the east, contrasting with the expectations of the serial-founder model<sup>8,9</sup>, and thus informing and constraining models of the initial expansion.
Correlation of the Erythrocyte Sedimentation Rate and Gold Complications in Rheumatoid Arthritis
Authors: L. Kalliomaki
Journal: Annals of the Rheumatic Diseases (1954)

Certain observations havebeenreported according towhichcomplications inassociation withgold therapy inrheumatoid arthritis wouldseemtobe inversely proportional totheerythrocyte sedimentation rate(Goldie, 1939; Ellman andothers, 1940; Lawrence, 1953). Thishasbeenexplained onthe basis that ahigh E.S.R. isgenerally seenintheactive exudative stage ofthedisease, andthatthegold, whichhasbecomeboundtotheglobulins, isthen abletopenetrate through thedamaged capillaries intothediseased tissue. Consequently thegold concentration intheskin, bonemarrow, andother tissues isunable toattain thesamelevels aswhenthe E.S.R. islowandthedisease isinaless active stage. Since this point isanimportant oneinclinical practice, Ihaveobserved thepossible correlation of goldcomplications andE.S.R. inpatients receiving continuous goldtherapy attheTurkuPolyclinic for Rheumatic Diseases. Material Theseries comprised 173patients. Thegold*was administered onceweekly during 10-12 weeksandthe 50-mg. limit wasnotexceeded. Whenatotal dosage of500mg.hadbeenreached, further administration was intheformofaso-called maintenance dosage of50mg. onceamonth. Results TheTableshowsthecorrelation between the incidence ofgoldcomplications andtheerythrocyte sedimentation rate.Inthose cases inwhichthere wereno complications ofthiskind,thelowest E.S.R. during goldtherapy wasnoted.Incases withcomplications, theE.S.R. registered during the occurrence of thecomplication was observed. Without exception allonsets ofdermatitis during the goldtherapy weretaken into consideration. Itching
Requirements for Both Rac1 and Cdc42 in Membrane Ruffling and Phagocytosis in Leukocytes
Authors: Dianne Cox, Peter Chang, Qing Zhang, P. Gopal Reddy, Gary M. Bokoch, Steven Greenberg
Journal: The Journal of Experimental Medicine (1997)

Specific pathways linking heterotrimeric G proteins and Fcγ receptors to the actin-based cytoskeleton are poorly understood. To test a requirement for Rho family members in cytoskeletal events mediated by structurally diverse receptors in leukocytes, we transfected the full-length human chemotactic peptide receptor in RAW 264.7 cells and examined cytoskeletal alterations in response to the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP), colony stimulating factor–1 (CSF-1), IgG-coated particles, and phorbol 12-myristate 13-acetate (PMA). Expression of Rac1 N17, Cdc42 N17, or the GAP domain of n-chimaerin inhibited cytoskeletal responses to FMLP and CSF-1, and blocked phagocytosis. Accumulation of F-actin– rich “phagocytic cups” was partially inhibited by expression of Rac1 N17 or Cdc42 N17. In contrast, PMA-induced ruffling was not inhibited by expression of Rac1 N17, but was blocked by expression of Cdc42 N17, indicating that cytoskeletal inhibition by these constructs was nonoverlapping. These results demonstrate differential requirements for Rho family GTPases in leukocyte motility, and indicate that both Rac1 and Cdc42 are required for Fcγ receptor– mediated phagocytosis and for membrane ruffling mediated by structurally distinct receptors in macrophages.
Regression of Amyloidosis Secondary to Rheumatoid Arthritis
Authors: R. A. Parkins, E. G. L. Bywaters
Journal: BMJ (1959)

AMYLOIDOSIS SECONDARYCross Memorial Hospital with severe generalized protein.The rheumatoid arthritis of two years' duration.At that time a transient epi she was severely ill, with widespread active joint involve-Comment.ment,high fever, and a sedimentation rate between 43 and * a 68 mm./hour.The liver and spleen were not enlarged rheumatoid s and no proteinuria was noted on repeated testing with disease, the s