tbuckley/cabot-search huggingface dataset:
Sequential Computerized Hepatobiliary Imaging During Percutaneous Transhepatic Biliary Drainage
Authors: F FALCHERO, M VALENTINI, E CIAMBELLOTTI, G BECCHI
Journal: Clinical Nuclear Medicine (1985)

Sequential computerized hepatobiliary imaging was performed in 11 jaundiced patients before, during, and after biliary decompression. The rates of plasma clearances and radionuclide accumulation in liver cells and biliary tree were calculated, in addition to the uptake and retention index.
Blood Groups and Human Genetics
Authors: Robert R. Race
Journal: JAMA (1960)

BLOOD groups used to mean the divisions made by the presence or absence of red cell antigens. Nowadays this seems rather a limited use of the term for blood can be grouped in several other ways: for example by the antigens of the white cells, most of which promise to be quite different from those of the red, by the antigens of the platelets, by the Gm antigens of the plasma, and by the chemistry of the hemoglobins and haptoglobins and transferrins. A new and most surprising way of dividing blood into two groups has recently been described by Steinberg and Giles: these authors find that samples of human plasma can be divided into two groups—those which do, and those which do not, inhibit a curious agglutination reaction which occurs when two mating types of the yeast<i>Hansenula wingei</i>are brought together. It looks very much as if the ability
TCTAP A-161 Impact of Percutaneous Coronary Intervention on 12-month Chronic Total Occlusion Outcomes in Patients with Non-lad Disease
Authors: Seung-Woon Rha, Byoung Geol Choi, Se Yeon Choi, Yoonjee Park, Akkala Raghavender Goud, Hu Li, Sunki Lee, Ji Bak Kim, Sung Il Im, Jin Oh Na, Cheol Ung Choi, Hong Euy Lim, Jin Won Kim, Eung Ju Kim, Chang Gyu Park, Hong Seog Seo, Dong Joo Oh
Journal: Journal of the American College of Cardiology (2014)
A NEW APPROACH FOR THE NON INVASIVE DETECTION OF RELATIVE CHANGES IN AORTIC REGURGITATION BETWEEN REST &amp; EXERCISE
Authors: Anil V. Shah, Eberhard Henze, Marcus Schwaiger, Heinrich Schelbert
Journal: Clinical Nuclear Medicine (1982)

Shah, Anil V.; Henze, Eberhard; Schwaiger, Marcus; Schelbert, Heinrich Author Information
Partial tandem duplication of <i>MLL</i> gene in acute myeloid leukemia with translocation (11;17)(q23;q12–21)
Authors: Shuichi Yamamoto, Masanori Nishi, Kazuto Taniguchi, Miyoko Imayoshi, Yoshiyasu Ogata, Manabu Iwanaga, Nana Sakai, Yuhei Hamasaki, Eiichi Ishii
Journal: American Journal of Hematology (2005)

Abstract Translocation 11q23 and MLL gene rearrangements are commonly observed in acute myeloid leukemia (AML) in association with the myelomonocytic or monocytic feature. We describe a case involving a 15‐year‐old patient with AML characterized by leukemic cells exhibiting translocation (11;17)(q23;q12–21) and MLL gene rearrangement. No fusion partner gene of the MLL gene was identified, including RAR α(17q12) or AF17 (17q21); however, a partial tandem duplication of the MLL exon 11/exon 10 was detected in leukemic cells via a 3′RACE method for detection of unknown partner genes. The patient has been in remission for more than 2 years without hematopoietic stem cell transplantation. Am. J. Hematol. 80:46–49, 2005. © 2005 Wiley‐Liss, Inc.
Modulation of cardiac tissue electrophysiological properties with light-sensitive proteins
Authors: Udi Nussinovitch, Rami Shinnawi, Lior Gepstein
Journal: Cardiovascular Research (2014)

Optogenetics approaches, utilizing light-sensitive proteins, have emerged as unique experimental paradigms to modulate neuronal excitability. We aimed to evaluate whether a similar strategy could be used to control cardiac-tissue excitability. A combined cell and gene therapy strategy was developed in which fibroblasts were transfected to express the light-activated depolarizing channel Channelrhodopsin-2 (ChR2). Patch-clamp studies confirmed the development of a robust inward current in the engineered fibroblasts following monochromatic blue-light exposure. The engineered cells were co-cultured with neonatal rat cardiomyocytes (or human embryonic stem cell-derived cardiomyocytes) and studied using a multielectrode array mapping technique. These studies revealed the ability of the ChR2-fibroblasts to electrically couple and pace the cardiomyocyte cultures at varying frequencies in response to blue-light flashes. Activation mapping pinpointed the source of this electrical activity to the engineered cells. Similarly, diffuse seeding of the ChR2-fibroblasts allowed multisite optogenetics pacing of the co-cultures, significantly shortening their electrical activation time and synchronizing contraction. Next, optogenetics pacing in an in vitro model of conduction block allowed the resynchronization of the tissue's electrical activity. Finally, the ChR2-fibroblasts were transfected to also express the light-sensitive hyperpolarizing proton pump Archaerhodopsin-T (Arch-T). Seeding of the ChR2/ArchT-fibroblasts allowed to either optogentically pace the cultures (in response to blue-light flashes) or completely suppress the cultures' electrical activity (following continuous illumination with 624 nm monochromatic light, activating ArchT). The results of this proof-of-concept study highlight the unique potential of optogenetics for future biological pacemaking and resynchronization therapy applications and for the development of novel anti-arrhythmic strategies.
ACCURATE BLOOD SUGAR DETERMINATIONS WITH ONE-TENTH AND FIVE-HUNDREDTHS CUBIC CENTIMETERS OF BLOOD
Authors: Rawson J. Pickard
Journal: JAMA (1930)

On the basis of our statistical comparison of blood sugar methods,<sup>1</sup>we offer a microtechnic of the Folin-Wu method as the best clinical technic for blood sugar determinations for the following reasons: 1. The technic is exceptionally accurate. There is only one micromeasurement, that of the blood, 0.10 or 0.05 cc. With blood containing from 75 to 150 mg. of dextrose per hundred cubic centimeters, we have shown by statistical methods that the Folin-Wu method has an average error not in excess of 2 per cent and a maximum error of 10 per cent. which is clinically negligible and needs no interpolation from the chart as is required with other blood sugar technics. By doubling the usual dilution of the blood we bring blood sugars of 300 mg. of dextrose within the range where readings are accurate and the wide divergences ordinarily appearing do not play a part. By
CCR5 Chemokine Receptor Variant in HIV-1 Mother-to-Child Transmission and Disease Progression in Children
Authors: M Misrahi, J P Teglas, N N'Go, M Burgard, M J Mayaux, C Rouzioux, J F Delfraissy, S Blanche
Journal: JAMA (1998)

for the French Pediatric HIV Infection Study GroupContext.-Studies suggest that adults with the CCR5⌬32 deletion are less likely to become infected with the human immunodeficiency virus (HIV) and to develop HIV-related disease progression, but the effect of the mutation in children is not known.Objective.-Tostudy the effect of the CCR5 chemokine receptor mutant allele on mother-to-child transmission of HIV type 1 (HIV-1) and subsequent disease progression in infected children.Design.-Multicenter, prospective study of infants born to mothers seropositive for HIV-1.Setting.-A total of 52 medical centers participating in the French Pediatric HIV Cohort studies.Participants.-TheCCR5⌬32 deletion was studied in 512 non-African children, born between 1983 and 1996 to HIV-1-infected mothers.Among them, 276 children were infected and 236 were not.Main Outcome Measures.-HIV-1infection status and, in infected children followed up since birth, incidence of category B and C disease events and severe immunosuppression as defined in the new pediatric Centers for Disease Control and Prevention (CDC) classification, according to CCR5 genotype.Results.-The 32-base pair deleted allele was detected at a frequency of 0.05.Only 1 infant, not infected by HIV-1, was homozygous for the ⌬32 deletion.The 49 heterozygous children (9.6% of the total; 95% confidence interval [CI], 7.1-12.2) were equally distributed into the infected (9.8%) and uninfected (9.3%) groups.The incidence of stage C symptoms in heterozygous infected children was 9% at 36 months vs 28% in children homozygous for the normal allele (PϽ.004).The proportion of children at 8 years old with no stage B or C symptoms was 49% for heterozygous children and 11% for children homozygous for the normal allele (PϽ.003).The progression of severe immune deficiency (CD4 Ͻ15%, CDC stage 3) was also significantly different between the 2 groups (PϽ.001).Conclusions.-Heterozygosity for the CCR5⌬32 deletion does not protect children from infection by the maternal virus but substantially reduces the progression of the disease in HIV-1-infected children.
Liposome Delivery of MicroRNA-145 to Mesenchymal Stem Cells Leads to Immunological Off-target Effects Mediated by RIG-I
Authors: Tommy A Karlsen, Jan E Brinchmann
Journal: Molecular Therapy (2013)

Synthetic microRNAs regulate gene expression when transfected into cells, and may be used in strategies for molecular therapy both in vitro and in vivo. Liposomal transfection reagents are frequently used as delivery vehicles in both settings. Here, we report on the immunological off-target effects observed following liposome transfection of synthetic microRNA-145 into human mesenchymal stem cells and human articular chondrocytes (hAC). The immune response was independent on endosome delivery and toll-like receptors (TLRs) but was mediated by retinoic acid inducible-gene 1 (RIG-I). Upregulation of immune genes required liposomal delivery, as no immune response was observed after electroporation of smiR-145 directly in to the cytosol, suggesting a new role of RIG-I. Immune response was seen both with blunt ended and 2-nucleotide 3' overhang versions of synthetic miR-145, and occurred in the absence of a 5'ppp cap. Mutations in a centrally placed poly (UUUU) sequence reduced, but did not abolish the immune response. Interestingly, exposure to liposomes alone led to upregulation of several immune genes, including RIG-I mRNA. However, this process was not mediated by RIG-I. This insight is important for researchers to avoid unexpected results from gene transfer experiments in vitro and unwanted immune responses following the use of lipid-based transfection reagents in vivo.
An oral treatment for iron overload in thalassemia?
Authors: K V Kowdley, A S Tavill
Journal: Hepatology (1996)

provide insight into the molecular pathogenesis.The 3. Okey AB, Riddick DS, Harper PA.Molecular biology of the aroextracellular matrix deposition and gene expression matic hydrocarbon (dioxin) receptor.Trends Pharmacol Sci need to be measured and compared with other well-1994
GW25-e5269 Rosuvastatin Protect Hcy-Iuduced Injury Against Oxidative Stress In Human Umbilical Vein Endothelial Cells
Authors: Hao Baoshun, Liu Xuelian, Liu Yong, Yu Shujie, Liu Dinghui, Qian Xiaoxian
Journal: Journal of the American College of Cardiology (2014)
Serum autoantibodies and immunoglobulins in hepatitis-associated antigen (HAA)-positive and -negative liver disease
Authors: F. J. Dudley, M. J. O'Shea, A. Ajdukiewicz, S. Sherlock
Journal: Gut (1973)

Serum immunoglobulin G, A, and M and serum antinuclear, mitochondrial, and smooth muscle antibody have been measured in 223 patients with hepatitis-associated antigen (HAA)-positive and -negative acute and chronic liver disease. In patients with acute hepatitis, chronic persistent hepatitis, and primary liver cell carcinoma, these indices failed to show any significant differences. However, in the group with chronic aggressive hepatitis the patients who were HAA negative had significantly higher levels of serum IgG, much higher titres of smooth muscle antibody, and often antinuclear and mitochondrial antibodies which were not found in the HAA-positive patients from this group. This suggests that different pathogenic mechanisms may be operative in HAA-positive and -negative chronic aggressive hepatitis.
Global elimination of mother to child HIV transmission is now achievable, say agencies
Authors: P. Moszynski
Journal: BMJ (2010)

For the first time the elimination of transmission of HIV from mothers to their babies is considered a realistic goal, according to a presentation at a summit in New York on the United Nations’ millennium development goals.

On 21 September the heads of Unicef, the World Health Organization, UNAIDS, and the Global Fund to fight AIDS, Tuberculosis and Malaria, together with representatives of donor governments and most affected countries, jointly launched a new initiative to promote intensified efforts to reach all mothers and children with the most effective interventions.

Unicef says that although the delivery of proved interventions “has effectively eliminated MTCT [mother to child transmission] in affluent countries, …
Report LXXXV: Some Further observations on the Life-History of Filaria Immitis, Leidy
Authors: Thos. L. Bancroft
Journal: BMJ (1904)

OF FILARIA .IMMITIS.[APR 9 1 sions, its anatomical course is in the main a progressive one, and the remittent stages often occur under treatment other than that of decapsulation.Of the above per cent.mortality, 26 per cent.died from chronic interstitial nephritis; and of the remainder, in 25 per cent.death occurred from the so-called chronic parenchymatous nephritis, and in 75 per cent." diffase" (?) nephritis was assigned as the cause.In acute nephritis, anuria, haematuria, puncture and decapsulation of the kidney is said to yield relief and to favourably affect the function of the other kidney.In these cases it is suggested that the improvement is due simply to
Methanogens outcompete sulphate reducing bacteria for H2 in the human colon.
Authors: A Strocchi, J Furne, C Ellis, M D Levitt
Journal: Gut (1994)

Methanogens and sulphate reducing bacteria compete for H2 in the human colon, and, as a result, faeces usually contain high concentrations of just one of these two organisms. There is controversy over which of these organisms wins the competition for H2, although theoretical data suggest that sulphate reducing bacteria should predominate. To elucidate this question experiments were undertaken in which sulphate enriched homogenates of human sulphate reducing faeces and methane producing faeces were incubated separately or mixed together. Co-incubation of sulphate reducing faeces with methanogenic faeces resulted in a sixfold reduction in the activity of the sulphate reducing bacteria (measured as sulphide production), whereas methane production was not inhibited by co-incubation with sulphate reducing bacteria. Methanogenic faeces also consumed H2 more rapidly and reduced the H2 tension of the homogenate to a lower value than did sulphate reducing faecal samples. In these experiments, methanogens seem to outcompete sulphate reducing bacteria for H2.
Angiogenesis and platelets: the clot thickens further
Authors: A BLANN
Journal: Cardiovascular Research (2004)

See article by Brill et al. [15] (pages 226–235) in this issue. 

Pathophysiogical aspects of platelet biology have, until recently, focussed on their relatively passive and reactive roles in minimising blood loss after injury, in contributing to the final stages of cardiovascular disease (largely in the inappropriate formation of life-threatening thrombosis), and on attempts in the clinic, the cardiac catheter suite, and on the ward to reduce the latter with anti-coagulant and anti-platelet agents. However, there is currently a growing awareness that another pathophysiological process, inflammation, may be important in atherosclerosis and acute coronary syndromes and, as a result, could have a role in platelet biology [1]. Among the various lines of evidence supporting this concept include the expression by platelets of integrin and adhesion molecules (such as P-selectin and CD154, the ligand for CD40) designed to enhance contact with the endothelium and leukocytes (possibly activating the transcriptional factor nuclear factor-κB) and the presence within the platelet of vasoactive molecules such as prostacyclins and thromboxanes, platelet-derived growth factor (PDGF), transforming growth factor-beta, and nitric oxide. Indeed, these and other data (e.g. adhesion of platelets to the endothelium prior … 

*Tel./fax: +44-121-507-5076. Email address: a.blann{at}bham.ac.uk
Hollywood Is Calling
Authors: Dorothy M. Bernstein
Journal: JAMA (1985)

You are one of a triad of distinctive young women I see in my practice. The three of you have one thing in common: a delusional system that tells you Hollywood awaits you. You do not know each other, and each of you has your own approach to such a challenge. You, Amelia, are the boldest of the three. You are the one most likely to take action. I remember when you decided to hitchhike to California to present yourself in person. Only you went in the direction of Mississippi instead. The police called me from Kansas to say they found you wandering the streets, penniless and bewildered. Our welfare system sent you a bus ticket and some money to return to this state. Although I was expecting to see you in my office once you reappeared, I did not hear about you for another month, when the police called
Laparoscopic Cholecystectomy: Surgical Procedure, Laparoscopic Cholecystectomy: Patient Education Program, Laparoscopic Nissen Fundoplication: Surgical Procedure, Laparoscopic Nissen Fundoplication: Patient Education Program, and Laparoscopic Inguinal Hernia Repair--Transabdominal and Balloon-Assisted Extraperitoneal Approaches: Surgical Procedure
Authors: M. S. Tucker
Journal: JAMA (1998)
67Ga SPECT/CT Aids in the Diagnosis of Occult Infected Common Iliac Artery Aneurysm
Authors: Mei-Fang Cheng, Kao-Lang Liu, Yu-Feng Lin, Kai-Yuan Tzen, Ruoh-Fang Yen
Journal: Clinical Nuclear Medicine (2013)

A 56-year-old woman, who had already suffered from systemic sclerosis and primary biliary cirrhosis, was presented with progressive pain at the right lower quadrant of abdomen, lower-grade fever, and leukocytosis for 4 days. Blood cultures did not yield pathogens. Abdominal ultrasonography revealed a right iliac artery aneurysm compressing the right kidney resulting in right hydronephrosis. 67Ga SPECT/CT demonstrated an intense hot area at the right common iliac artery, suggestive of an infected aneurysm. Contrast-enhanced CT showed concordant findings. This case illustrates the value of 67Ga SPECT/CT in discriminating pathologic uptake of the abdomen from physiologic activity of the bowels.
Response to Letter Regarding Article, “Randomized Study of the Crush Technique Versus Provisional Side-Branch Stenting in True Coronary Bifurcations: The CACTUS (Coronary Bifurcations: Application of the Crushing Technique Using Sirolimus-Eluting Stents) Study”
Authors: Antonio Colombo, Azeem Latib, Flavio Airoldi, Ezio Bramucci, Salvatore Saccà, Roberto Violini, Corrado Lettieri, Roberto Zanini, Imad Sheiban, Leonardo Paloscia, Eberhard Grube, Joachim Shofer, Leonardo Bolognese, Mario Orlandi, Giampaolo Niccoli
Journal: Circulation (2009)
Meningococcal endotoxin in lethal septic shock plasma studied by gas chromatography, mass-spectrometry, ultracentrifugation, and electron microscopy.
Authors: P Brandtzaeg, K Bryn, P Kierulf, R Ovstebø, E Namork, B Aase, E Jantzen
Journal: Journal of Clinical Investigation (1992)

We have compared gas chromatography and mass spectrometry (GC-MS) analysis with the Limulus amebocyte lysate (LAL) assay to quantify native meningococcal lipopolysaccharides (LPS) in five patient plasmas containing greater than 5 micrograms/liter by LAL. 3-Hydroxy lauric acid (3-OH-12:0) was used as a specific lipid A marker of neisserial LPS. The quantitative LAL results were confirmed by GC-MS (r = 0.98, P = 0.006). Seven patient plasmas were centrifuged at 103,000 g and the sedimentation behavior of native LPS compared with reference plasma proteins and with apo A1 and apo B100 representing high and low density lipoproteins. After 15 min of centrifugation, 84 +/- 2% (mean +/- SE) of the recovered LPS were found in the lower one-third of the centrifuged volume, whereas 6 +/- 1% remained in the upper one-third volume, indicating that meningococcal endotoxin circulates as complexes with high sedimentation coefficients. Bacterial outer membrane fragments were detected in the bottom fractions of three patient plasmas examined by means of electron microscopy. In three patient plasmas ultracentrifuged for 60 min at 103,000 g, the levels of apo A1 and apo B100 revealed minor changes, whereas only 1 +/- 1% of the recovered LPS remained in the upper one-third and 91 +/- 2% were found in the lower one-third volume. Few bioreactive LPS appear to be complexed with high and low density lipoproteins in meningococcal septic shock plasma.
Abstract 2136: Long-term metformin use reduces gastric cancer risk in type 2 diabetics without insulin treatment: a nationwide cohort study
Authors: Soo-Jeong Cho, Young-Il Kim, So Young Kim, Jong-Hyock Park, Il Ju Choi, You Jin Lee, Eun Kyung Lee, Myeong-Cherl Kook, Chan Gyoo Kim, Keun Won Ryu, Young-Woo Kim
Journal: Cancer Research (2014)

Abstract Background: Metformin use has been associated with a decreased incidence and mortality of various cancers. Aim: The aim of this study was to evaluate the association between metformin use and gastric cancer. Methods: We randomy selected 100,000 type 2 diabetic patients from the 2004 Korean National Health Insurance claim database, and assessed gastric cancer incidence among 39,989 patients (aged 30-97 years) who were regularly treated with anti-diabetic drugs and followed-up from 2004 to 2010. In total, 26,690 patients had used metformin out of 32,978 diabetics who had not regularly used insulin (insulin non-users), and 5,855 patients had used metformin out of 7,011 regular insulin users. Results: Patients who used metformin showed a lower incidence of gastric cancer than those who did not use metformin, in insulin non-users (P=0.047, log-rank test). However, in patients on regular insulin, there was no difference of gastric cancer incidence according to metformin use. In insulin non-users, the adjusted hazard ratio (AHR) for metformin use was 0.73 (95% confidential interval [CI], 0.53-1.01) with borderline statistical significance (P=0.059). Duration of metformin use was associated with the reduction of gastric cancer risk (AHR, 0.88; 95% CI 0.81-0.96, P=0.003), especially in patients that used metformin for more than 3 years (AHR, 0.57; 95% CI, 0.37-0.87; P=0.009). Conclusion: Metformin use &amp;gt;3 years in type 2 diabetics who do not use insulin is associated with significantly reduced gastric cancer risk. Citation Format: Soo-Jeong Cho, Young-Il Kim, So Young Kim, Jong-Hyock Park, Il Ju Choi, You Jin Lee, Eun Kyung Lee, Myeong-Cherl Kook, Chan Gyoo Kim, Keun Won Ryu, Young-Woo Kim. Long-term metformin use reduces gastric cancer risk in type 2 diabetics without insulin treatment: a nationwide cohort study. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2136. doi:10.1158/1538-7445.AM2014-2136