tbuckley/cabot-search huggingface dataset:
Mass spectrometric peptide mapping analysis and structural characterization of dihydrodiol dehydrogenase isoenzymes.
Authors: C Gauss, J Klein, K Post, D Suckau, K Schneider, H Thomas, F Oesch, M Przybylski
Journal: Environmental Health Perspectives (1990)

The direct molecular weight determination and structural analysis of polypeptides and peptide mixtures have become amenable by the recent development of fast atom bombardment (FABMS) and 252Cf-plasma desorption (PDMS) mass spectrometry. FABMS and PDMS peptide mapping, i.e., the direct analysis of peptide mixtures resulting from proteolytic digestion, have been developed as powerful methods for the structural characterization of epoxide-metabolizing isoenzymes. The major advantage of this approach is provided by the selectivity of the endoproteolytic cleavage, combined with the specific and accurate molecular weight determination of complex digest mixtures containing peptides up to several thousands daltons in size. Furthermore, the mass spectrometric peptide mapping analysis can be combined with a range of protein-chemical modification reactions and with sequential degradation such as by carboxypeptidases. Both FABMS and PDMS peptide mapping have already been successfully applied to the structural differentiation of glutathione transferase and epoxide hydrolase isoenzymes in cases where references sequence data for at least one isoenzyme form was available. In the application described here, for a series of dihydrodiol dehydrogenase (DDH) isoenzymes with hitherto undetermined primary structures, a direct correlation between the structural differentiation from peptide mapping data and differences in their substrate specificities could be demonstrated. The mass spectrometric peptide mapping analysis of isoenzymes proved to be an efficient basis for the elucidation of the structure of one major DDH isoenzyme form; partial sequence data for this protein are reported.
Standing Views to Differentiate Gallbladder or Bile Leak from Duodenal Activity on Cholescintigrams
Authors: JEAN LETTE, MICHEL MORIN, FRANCOISE HEYEN, ALINE PAQUET, ANDRE LEVASSEUR
Journal: Clinical Nuclear Medicine (1990)

Confusing a gallbladder or bile leak with duodenal activity is a common source of error in the interpretation of cholescintigrams. Techniques such as supplementary oblique views, delayed views, the “water test,” dynamic display and combined cholescintigraphy and ultrasound have been used with varying degrees of success. As illustrated in two cases, supplementary standing views provide a simple alternative method to differentiate gallbladder from small bowel activity.
Foreign Medical Schools
Authors: A. E. Ross
Journal: JAMA (1972)

To the Editor.—  The article by Mason on Foreign Medical Schools (217:1845, 1971) states that  salaries in all schools are paid by state governments. I have personal knowledge that this is an error of fact in respect to the Autonomous University of Guadalajara. This may be correct for the other schools listed and the rest of the data input may be factual; however, the model is ultimately used to make comparisons that are questionable, especially in the accompanying EDITORIAL by Nicholson (217:1857, 1971). My reasons for disagreement are the following: (1) Attrition in Mexico occurs after first year with less rigid selection and more wastage than in the United States and Canada. A more valid comparison would include students in the senior years rather than only freshman students. (2) The reservation regarding the definition of fulltime faculty stated in the reference article used for comparison (210:1476,
Health Effects of Energy-Generating Sources
Authors: Ted L. Guidotti
Journal: JAMA (1979)

<h3>To the Editor.—</h3> The American Medical Association Council on Scientific Affairs does not appear to have performed its evaluation of the health effects of energy-generating sources with a view toward the ultimate purpose of its analysis (240:2193, 1978). The document is of interest as a summary of the occupational health risks associated with nuclear and fossil-fuel power production in the recent past, but it is not adequate to serve as a guide to future policy for the following reasons: The risks attributed to coal mining reflect hazards associated with underground mining.<sup>1,2</sup>The expansion of coal mining under the National Energy Plan, especially in the western states where the potential for future production is greatest, will be preponderantly surface mining.<sup>3,4</sup>The AMA council report bases its evaluation on extrapolation from the most hazardous coal extraction technology rather than the technology that will in fact be employed. Surface mining
Tumors of the Peripheral Nervous System
Authors: I. M. Ariel
Journal: CA A Cancer Journal for Clinicians (1983)

Most tumors (benign and malignant) do not arise from the nerves per se, but from the supporting cells; tumors arising from the cells of Schwann are termed schwannoma or neurilemoma--benign or malignant. Surgical extirpation is the only way of treating these tumors. Radiation therapy can offer significant palliation and prolongation of life, but no cures have been observed. Other forms of therapy (chemotherapy, immunotherapy, etc.) have little to offer at this time. Benign tumors can be treated by local surgical extirpation; malignant tumors must be radically resected, including major amputations where indicated. Certain dermatomes have a proclivity to produce tumors, and new growths (benign and malignant) proximal to the one clinically apparent can be expected in certain patients. Neurofibromatosis (von Recklinghausen's disease) is a genetic error of metabolism with a proclivity to produce multiple neurofibrosarcomas, and in about 10 percent of the patients, malignant neurilemomas. Of 100 patients with malignant neurilemomas treated by the author, 74 were considered determinate; among them, the 10-year "cure" rate was 32 percent. Patients with von Recklinghausen's disease had almost as good a 10-year survival rate as those with solitary malignant schwannoma (30 percent vs 39 percent).
HYGIENE AND DIETETICS IN DIABETES MELLITUS.
Authors: FRANCIS E. CARROLL
Journal: JAMA (1898)

If perfect culture of mind and body was to be had, a perfect knowledge of the laws of life, an intellect and body trained, the moral faculties of man in balanced order, the human race would not perish under a grievous yoke of disease; the youth of man would be protected and become more perfect; decay of life would be blended with happiness of perfect knowledge; life would become more vigorous, disease more remote. Life is an entity, a constant growth, a resultant waste, a potential energy an eventual expenditure. No agent has been found to arrest the waste or crystallize the tissues of growth and vigor from decay. To do so would be death itself, for life is an incessant change, its vital energy dependent upon the laws of nature, a neglect of which inevitably produces lamentable results. To prolong the period of vital energy, the improvement of the
Intercellular distribution of HLA-DR and T6 antigens in the epidermis of various dcrmatoses
Authors: R. Willemze, A. Dijkstra, M. Nugteren
Journal: British Journal of Dermatology (1985)

Journal Article Intercellular distribution of HLA‐DR and T6 antigens in the epidermis of various dcrmatoses Get access R. Willemze, R. Willemze Department of Dermatology, University Hospital, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar A. Dijkstra, A. Dijkstra Department of Dermatology, University Hospital, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar M. Nugteren M. Nugteren Department of Dermatology, University Hospital, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 113, Issue 6, 1 December 1985, Pages 802–804, https://doi.org/10.1111/j.1365-2133.1985.tb02420.x Published: 01 December 1985
Arbutamine echocardiography: Efficacy and safety of a new pharmacologic stress agent to induce myocardial ischemia and detect coronary artery disease
Authors: Jerald L. Cohen, Kwan L. Chan, Wybren Jaarsma, David S. Bach, David W.M. Muller, Mark R. Starling, William F. Armstrong
Journal: Journal of the American College of Cardiology (1995)
Office-Based Treatment of Opioid-Dependent Patients
Authors: David A. Fiellin, Patrick G. O'Connor
Journal: New England Journal of Medicine (2002)

A 30-year-old man reports a two-year history of heroin use. For the past year, he has been using intranasal heroin every day. He has undergone detoxification twice at a local opioid-abuse treatment program but began using heroin within two days after discharge each time. He has heard of methadone but fears that he will lose his business if he is recognized attending the local program. How should this patient be treated?
A Randomized Trial of Laser-assisted Uvulopalatoplasty in the Treatment of Mild Obstructive Sleep Apnea
Authors: Kathleen A. Ferguson, Kim Heighway, Ralph R. F. Ruby
Journal: American Journal of Respiratory and Critical Care Medicine (2002)

Laser-assisted uvulopalatoplasty (LAUP) is an outpatient surgical treatment for snoring and obstructive sleep apnea (OSA), but to date, no controlled trials have been published. Forty-five subjects with mild OSA (apnea/hypopnea index [AHI], 10–27 per hour) were randomized to LAUP or to no treatment (control group). The AHI post-LAUP was reduced by 21% overall and to 10 or less per hour in 5 of 21 subjects (24%). Four of 24 subjects in the control group (16.7%) had an AHI of 10 or less per hour at outcome. The AHI decreased with the LAUP compared with no change with the control group at outcome. Ten subjects (48%) reported significantly improved snoring after the LAUP. There was no improvement in excessive daytime sleepiness, but there was a small improvement in quality of life (unless side effects were included in the quality of life score). Side effects were common, but serious complications did not occur. LAUP surgery is effective in some subjects with mild OSA for the treatment of snoring, but the reduction in AHI and the level of symptomatic improvement were minor overall.
Hyperpyrexia From Drug Combinations
Authors: Robert J. Westlake
Journal: JAMA (1973)

<h3>To the Editor.—</h3> An important side effect of phenothiazine-anticholinergic combination is interference with the body thermoregulatory system resulting in hyperpyrexia and occasionally death in patients exposed to high environmental temperature and humidity. During a recent hot and humid period in Philadelphia, we saw three patients with severe hyperpyrexia while taking psychotrophic medications. These cases are briefly reported to acquaint physicians with this reversible but potentially fatal side effect of a common psychiatric pharmacotherapy.<sup>1</sup> <h3>Report of Cases.—Case 1.—</h3> A 45-year-old chronic schizophrenic woman had been treated with 500 mg chlorpromazine, 200 mg chlorprothixene, and 6 mg benztropine mesylate daily in divided doses for several months. She lived in a non-air-conditioned apartment but had not worked outdoors on the day of admission. She was stuporous but arousable, with a blood pressure of 100/50 mm Hg; temperature, 41.1 C (rectally); pulse, 120 beats per minute; and respirations, 22. Other findings were
Determinants of Growth in Diabetic Pubertal Subjects
Authors: I Zachrisson, K Brismar, K Hall, M Wallensteen, G Dahlqvist
Journal: Diabetes Care (1997)

OBJECTIVE To analyze the relationship among metabolic control, IGF-I, and growth in pubertal diabetic subjects. RESEARCH DESIGN AND METHODS In 72 diabetic children, we have studied the pattern of change of IGF-I, IGF-I SD score, IGF binding protein (BP)-1, and growth rate in different pubertal stages and have analyzed their relation to age sex, weight/length index, HbA1c, insulin concentration, insulin dose, and dehydroepiandrosteronesulfate (DHEAS). RESULTS The serum IGF-I values increased up to Tanner stage 4 and thereafter decreased, whereas IGFBP-1 showed the inverse pattern. When transforming the IGF-I values into SD scores, correcting for age, sex, and pubertal stage, it was shown that the deviation from normal values increased with increasing pubertal stage in boys, but was equal in stages 3–5 in girls. Using multiple regression analysis, HbA1c, insulin dose, and DHEAS were significantly correlated to IGF-I SD score (R2 = 0.253, P = 0.001). IGFBP-I levels in the afternoon were within normal range. LogIGFBP-1 showed an inverse correlation, to insulin concentration in single correlation (r = −0.26, P = 0.02). In single correlation, growth rate correlated significantly to insulin dose (r = 0.25, P = 0.03). In a multiple regression analysis, only DHEAS and IGF-I SD score were found to be significantly correlated to growth rate (R2 = 0.370, P &amp;lt; 0.001). The 18 adolescents who had reached their final height did not deviate from their target final height, according to their recorded growth since birth. CONCLUSIONS In a group of fairly well-controlled diabetic children, the normal increase in IGF-I during puberty is blunted. Despite decreased IGF-I levels, target final height was attained, probably because of adequate insulin compensation leading to normal IGFBP-l, thus adequate bioavailability of IGF-I. Our results point out the importance of sufficient exogenous insulin in the period of rapid linear growth.
Meta-Analysis and Bouillabaisse
Authors: Franz H. Messerli
Journal: Annals of Internal Medicine (1996)

Letters15 September 1996Meta-Analysis and BouillabaisseFranz H. Messerli, MDFranz H. Messerli, MDOchsner Clinic and Alton Ochsner Medical Foundation, New Orleans, LA 70121Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-125-6-199609150-00031 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:As Drs. Ghali and Hershman point out, Psaty and colleagues attempted to avoid an indication bias. No matter how carefully a retrospective case control-study is done, however, an indication bias can never be ruled out: In retrospect it is impossible to second guess the reason a specific cardiovascular drug was prescribed for a given patient. In another report from the same patient cohort [1], the authors freely admit that “as expected those with cardiovascular disease were more likely to be taking calcium channel blockers and β blockers. …” Because all patients had hypertension (which, by definition, is a ...References1. Psaty BM, Lee M, Savage PJ, Rutan GH, German PS, Lyles M. Assessing the use of medications in the elderly: methods and initial experience in the Cardiovascular Health Study. The Cardiovascular Health Study Collaborative Research Group. J Clin Epidemiol. 1992; 45:683-92. Google Scholar2. Psaty BM. U.S. Food and Drug Administration hearing. 25 January 1996. Google Scholar3. Wilson DC, Schwarts GL, Textor SC, Zachariah PK, Sheps SG. Precipitous fall in blood pressure in the treatment of chronic hypertension. Proceedings of the 5th International Symposium on Calcium Antagonists: Pharmacology and Clinical Research, Houston, Texas, September 1991. Google Scholar4. Hoes AW, Grobbee DE, Lubsen J, Man in 't Veld AJ, van der Does E, Hofman A. Diuretics, β-blockers, and the risk for sudden cardiac death in hypertensive patients. Ann Intern Med. 1995; 123:481-7. Google Scholar5. Siscovick DS, Raghunathan TE, Psaty BM, Koepsell TD, Wicklund KG, Lin X, et al. Diuretic therapy for hypertension and the risk of primary cardiac arrest. N Engl J Med. 1994; 330:1852-7. Google Scholar Author, Article, and Disclosure InformationAuthors: Franz H. Messerli, MDAffiliations: Ochsner Clinic and Alton Ochsner Medical Foundation, New Orleans, LA 70121 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byQuality control of B-lines analysis in stress Echo 2020Conflict of Interest in the Debate over Calcium-Channel Antagonists 15 September 1996Volume 125, Issue 6Page: 519KeywordsCalciumCardiovascular diseasesDiureticsDrugsHypertensionIschemiaMortalitySafetySafety studiesTachycardia ePublished: 15 August 2000 Issue Published: 15 September 1996 Copyright & PermissionsCopyright © 1996 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Array Comparative Genomic Hybridization Reveals Distinct DNA Copy Number Differences between Gastrointestinal Stromal Tumors and Leiomyosarcomas
Authors: Leonardo A. Meza-Zepeda, Stine H. Kresse, Ana H. Barragan-Polania, Bodil Bjerkehagen, Hege O. Ohnstad, Heidi M. Namløs, Junbai Wang, Bjørn E. Kristiansen, Ola Myklebost
Journal: Cancer Research (2006)

Abstract Leiomyosarcomas are spindle cell tumors showing smooth muscle differentiation. Until recently, most gastrointestinal stromal tumors (GIST) were also classified as smooth muscle tumors, but now GISTs are recognized as a separate entity, defined as spindle cell and/or epithelioid tumors localized in the gastrointestinal tract. Using microarray-based comparative genomic hybridization (array CGH), we have created a detailed map of DNA copy number changes for 7 GISTs and 12 leiomyosarcomas. Considerable gains and losses of chromosomal segments were observed in both tumor types. The most frequent aberration observed in GISTs was loss of chromosomes 14 and 22, with minimal recurrent regions in 14q11.2-q32.33 (71% of the tumors) and 22q12.2-q13.31 (100%). In leiomyosarcomas, frequent loss of chromosome 10 and 13q was observed, with minimal recurrent regions in 10q21.3 (75%) and 13q14.2-q14.3 (75%). Recurrent high-level amplification of 17p13.1-p11.2 was detected in leiomyosarcomas. Expression profiling using cDNA microarrays revealed four candidate genes in this region with high expression (AURKB, SREBF1, MFAP4, and FLJ10847). Altered expression of AURKB and SREBF1 has been observed previously in other malignancies. Hierarchical clustering of all samples separated GISTs and leiomyosarcomas into two distinct clusters. Statistical analysis identified six chromosomal regions, 1p36.11-p13.1, 9q21.11-9q34.3, 14q11.2-q23.2, 14q31.3-q32.33, 15q24.3-q26.3, and 22q11.21-q13.31, which were significantly different in copy number between GISTs and leiomyosarcomas. Our results show the potential of using array comparative genomic hybridization to classify histologically similar tumors such as GISTs and leiomyosarcomas. (Cancer Res 2006;66(18):8984-93)
Pulsatile intermittent intravenous insulin therapy for attenuation of retinopathy and nephropathy in type 1 diabetes mellitus
Authors: Larry A. Weinrauch, Jennifer Sun, Ray E. Gleason, Guenther H. Boden, R.H. Creech, George Dailey, Frank P. Kennedy, Matthew R. Weir, John A. D'Elia
Journal: Metabolism (2010)
REPORTING OF ACCIDENTS FROM LOCAL ANESTHETICS
Authors: Torald Sollmann, R. A. Hatcher
Journal: JAMA (1918)

<h3>To the Editor:</h3> —The Committee on Therapeutic Research of the Council on Pharmacy and Chemistry of the American Medical Association has undertaken a study of the accidents following the clinical use of local anesthetics, especially those following ordinary therapeutic doses. It is hoped that this study may lead to a better understanding of the cause of such accidents, and consequently to methods of avoiding them, or, at least, of treating them successfully when they occur. It is becoming apparent that several of the local anesthetics. if not all of those in general use, are prone to cause death or symptoms of severe poisoning in a small percentage of those cases in which the dose used has been hitherto considered quite safe. The infrequent occurrence of these accidents and their production by relatively small doses point to a peculiar hypersensitiveness on the part of those in whom the accidents occur. The
Local immunodiagnosis of pulmonary tuberculosis by enzyme-linked immunospot
Authors: C. Jafari, M. Ernst, A. Strassburg, U. Greinert, B. Kalsdorf, D. Kirsten, C. Lange
Journal: European Respiratory Journal (2007)

Lymphocytes are crucial in the immune defence against Mycobacterium tuberculosis (MTB) infection. The aim of the present study was to ascertain whether or not MTB-specific lymphocytes are selectively compartmentalised in the lungs of patients with minimal active pulmonary tuberculosis (PTB). Patients with smear-negative MTB-culture-confirmed PTB were prospectively recruited. Differential cell counts, immunophenotyping with monoclonal antibodies directed against the cell surface markers CD4, CD8, CD4CD45RA, CD4CD45R0, CD38, human leukocyte antigen DR, CD19, CD3, CD57 and CD16 and MTB-specific enzyme-linked immunospot assays of peripheral blood mononuclear cells and bronchoalveolar lavage (BAL) mononuclear cells with 6-kDa early secretory antigenic target and culture filtrate protein 10 were performed. Among 12 patients with culture-confirmed smear-negative PTB, no differences were found in the distribution of total CD4 or CD8 T-cells in peripheral blood or BAL fluid (BALF). Activated human leukocyte antigen-DR-positive cells, as well as memory CD4CD45R0-positive T-cells, were expanded among cells of the BALF. Compared with a group of control patients with alternative pulmonary pathologies, there was no significant difference in lymphocyte subpopulations. However, 6-kDa early secretory antigenic target- and culture filtrate protein 10-specific lymphocytes were more concentrated, with a median BALF:peripheral blood ratio of 9.9 and 8.9, respectively, in patients with PTB. Mycobacterium tuberculosis-specific T-cells are highly selectively compartmentalised at the site of infection in active pulmonary tuberculosis.
The Natural History of Urate Overproduction in Sickle Cell Anemia
Authors: HERBERT S. DIAMOND
Journal: Annals of Internal Medicine (1979)

Serum uric acid and uric acid excretion were studied in 95 patients with sickle cell anemia ranging in age from 17 months to 45 years to ascertain the natural history of urate overproduction. Hyperuricemia was infrequent in children with sickle cell anemia, but was found in 26 of 67 adults (39%). Thirty-six patients studied in a clinical research center had a mean urate clearance of 9.1 ± 0.8 mL/min. Patients with sickle cell anemia were often normouricemic despite urate overproduction. Normouricemia was maintained by increased urate clearance, which was attributed to increased urate secretion. The hyperuricemic patients had decreased urate clearance with decreased pyrazinamide-suppressible urate clearance. Para-aminohippurate clearance was decreased to 634 mL/min in the hyperuricemic patients with sickle cell anemia compared with 853 mL/min in normouricemic hyperuricosuric subjects with sickle cell anemia. Hyperuricemia occurs only in patients who develop altered renal tubular function with diminished urate clearance secondary to diminished urate secretion.
Intestinal permeation enhancement of docetaxel encapsulated into methyl-β-cyclodextrin/poly(isobutylcyanoacrylate) nanoparticles coated with thiolated chitosan
Authors: Silvia Mazzaferro, Kawthar Bouchemal, Rym Skanji, Claire Gueutin, Hélène Chacun, Gilles Ponchel
Journal: Journal of Controlled Release (2012)
A piece of my mind. Medicine man
Authors: N. Haukebo
Journal: JAMA (1991)

The young couple who came into my office that day were good-looking and well dressed. The woman was slender and wore a subtly flowered dress that set off her brunette coloring. The man was more casually attired in knit shirt, linen slacks, and loafers. They could have been models on assignment. They were, in fact, the first Native Americans from the nearby reservation to come to our mental health center. The first thing that became apparent was that they were not on the best of terms. The second was that the young woman was not going to utter a word; not then and, as it turned out, not the next day. When I addressed her, she shook her head firmly. It was up to the man to explain that she was along solely as an observer. When I asked the man (let's call him Frank) how I could help, he