tbuckley/cabot-search huggingface dataset:
Reduced uptake of oxidized low density lipoproteins in monocyte-derived macrophages from CD36-deficient subjects.
Authors: S Nozaki, H Kashiwagi, S Yamashita, T Nakagawa, B Kostner, Y Tomiyama, A Nakata, M Ishigami, J Miyagawa, K Kameda-Takemura
Journal: Journal of Clinical Investigation (1995)

To clarify the physiological roles of CD36 as an oxidized low density lipoprotein (OxLDL) receptor, we analyzed the monocyte-derived macrophages from normal and two CD36-deficient subjects, since we identified the molecular abnormalities (Kashiwagi, H., Y. Tomiyama, Y. Kosugi, M. Shiraga, R. H. Lipsky, Y. Kanayama, Y. Kurata, and Y. Matsuzawa 1994. Blood. 83:3545-3552; and Kashiwagi, H., Y. Tomiyama, S. Honda, S. Kosugi, M. Shiraga, N. Nagao, S. Sekiguchi, Y. Kanayama, Y. Kurata, and Y. Matsuzawa. 1995. J. Clin. Invest. 95:1040-1046). Scatchard analysis of 125I-OxLDL binding showed a linear plot and the maximum binding was lower by approximately 40% in the macrophages from subjects with CD36 deficiency than those from normal controls. Competition studies showed that the uptake of 125I-OxLDL was suppressed by OKM5, an antibody against CD36, by 53% in normal control macrophages, but not in the CD36-deficient macrophages. After incubation with OxLDL for 24 h, cholesteryl ester mass accumulation was reduced by approximately 40% in the macrophages from CD36-deficient subjects than those from normal controls. These results suggest that CD36 is one of the physiological receptors for OxLDL. Since specific binding of OxLDL was only reduced by approximately 40% in spite of the complete deficiency of CD36, several other receptors also may have some role in OxLDL uptake. Further studies will be needed to assess the quantitative role of CD36 in foam cell formation in vivo.
Abstract 5537: Quantitative multiplexed SRM analysis of oncogenic receptors in FFPE colorectal carcinoma tissue
Authors: Todd Hembrough, Sheeno Thyparambil, Wei-Li Liao, Marlene Darfler, David Krizman, Richard Adams, Jon Burrows
Journal: Cancer Research (2012)

Abstract Multiple receptor tyrosine kinases are the target of either approved drugs (Her2, EGFR) or robust clinical development efforts (cMet, IGF1R, Her3, etc). In many cases initial drug response is followed by resistance and tumor progression. There are many mechanisms which have been proposed for this including co-expression of alternate RTKs that continue to stimulate tumor growth in the presence of EGFR/Her2 inhibition. Because of the potential that co-expression of oncogenic receptors mediates resistance in these clinical trials, we have developed a panel of new SRM assays which measure the expression of these critical RTKs in multiplex from FFPE tissues. These assays are based on the Liquid Tissue®-SRM technology platform. This approach enables relative and absolute quantification of proteins and their phosphorylation status directly in formalin fixed paraffin embedded (FFPE) tissue. Here we describe the quantitative multiplexed analysis of EGFR, IGF1R and cMet expression in a cohort of 75 archival FFPE colorectal cancer tumors from Cetuximab treated patients. This analysis identified 7 different fingerprints of expression of these three targets. Since each of these targets has either approved inhibitors, or highly active clinical development programs, we are hopeful that this analytical approach can help to identify patients who are most likely to respond to single or combination therapies of RTK inhibitors Follow up studies are underway to expand the RTK- multiplex, repeating EGFR, IGF1R and cMet and adding Her2, Her3, Her4, DR5, cSrc, and other drug targets into the multiplex. Our intention is to use this broader multiplex to re-analyze these patients and attempt to correlate the multiplexed expression of these targets with drug response data. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5537. doi:1538-7445.AM2012-5537
Phase II Trial of Bevacizumab and Irinotecan in Recurrent Malignant Glioma
Authors: James J. Vredenburgh, Annick Desjardins, James E. Herndon, Jeannette M. Dowell, David A. Reardon, Jennifer A. Quinn, Jeremy N. Rich, Sith Sathornsumetee, Sridharan Gururangan, Melissa Wagner, Darell D. Bigner, Allan H. Friedman, Henry S. Friedman
Journal: Clinical Cancer Research (2007)

Abstract Purpose: Recurrent grade III-IV gliomas have a dismal prognosis with minimal improvements in survival seen following currently available salvage therapy. This study was conducted to determine if the combination of a novel antiangiogenic therapy, bevacizumab, and a cytotoxic agent, irinotecan, is safe and effective for patients with recurrent grade III-IV glioma. Experimental Design: We conducted a phase II trial of bevacizumab and irinotecan in adults with recurrent grade III-IV glioma. Patients with evidence of intracranial hemorrhage on initial brain magnetic resonance imaging were excluded. Patients were scheduled to receive bevacizumab and irinotecan i.v. every 2 weeks of a 6-week cycle. Bevacizumab was administered at 10 mg/kg. The dose of irinotecan was determined based on antiepileptic use: patients taking enzyme-inducing antiepileptic drugs received 340 mg/m2, whereas patients not taking enzyme-inducing antiepileptic drugs received 125 mg/m2. Toxicity and response were assessed. Results: Thirty-two patients were assessed (23 with grade IV glioma and 9 with grade III glioma). Radiographic responses were noted in 63% (20 of 32) of patients (14 of 23 grade IV patients and 6 of 9 grade III patients). The median progression-free survival was 23 weeks for all patients (95% confidence interval, 15-30 weeks; 20 weeks for grade IV patients and 30 weeks for grade III patients). The 6-month progression-free survival probability was 38% and the 6-month overall survival probability was 72%. No central nervous system hemorrhages occurred, but three patients developed deep venous thromboses or pulmonary emboli, and one patient had an arterial ischemic stroke. Conclusions: The combination of bevacizumab and irinotecan is an active regimen for recurrent grade III-IV glioma with acceptable toxicity.
Measles Vaccine Strains for Virotherapy of Non–Small-Cell Lung Carcinoma
Authors: Manish R. Patel, Blake A. Jacobson, Holly Belgum, Ahmad Raza, Ahad Sadiq, Jeremy Drees, Hengbing Wang, Joseph Jay-Dixon, Ryan Etchison, Mark J. Federspiel, Stephen J. Russell, Robert A. Kratzke
Journal: Journal of Thoracic Oncology (2014)
Effective Pain Treatment Promotes Activities
Authors: Lynne Lamberg
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
In anticoagulated patients with AF, HAS-BLED predicted major bleeding better than CHADS<sub>2</sub>and CHA<sub>2</sub>DS<sub>2</sub>-VASc
Authors: Maura Marcucci
Journal: Annals of Internal Medicine (2014)

Source Citation Roldan V, Marín F, Manzano-Fernández S, et al. The HAS-BLED score has better prediction accuracy for major bleeding than CHADS2 or CHA2DS2-VASc scores in anticoagulated patients with atrial fibrillation. J Am Coll Cardiol. 2013;62:2199-204. 24055744
Characteristics of Nonbacteremic Pseudomonas Pneumonia
Authors: JAMES R. TILLOTSON
Journal: Annals of Internal Medicine (1968)

The distinguishing clinical course, laboratory findings, and morbid anatomy of nonbacteremic pseudomonas pneumonia is described. These infections occurred in ten patients (eight of whom ultimately died) who were admitted to the Detroit General (Receiving) Hospital during a 30-month surveillance from 1963 through 1965. Chronic lung or heart disease was a cardinal predisposing condition that apparently increased their susceptibility to infection with Bacillus pyocyaneus. In a few, prior administration of antibiotics may have contributed to colonization of upper respiratory passages by Pseudomonas. Apprehension, confusion, cyanosis, relative bradycardia, reversal of the usual diurnal temperature curve, and abnormal liver and kidney function were striking. At X ray a diffuse nodular lower lobe bronchopneumonia with microabscesses was seen. Pathologically, microabscesses and focal hemorrhage were noted. There was no necrotizing bacillary vasculitis nor thrombosis of pulmonary vessels as has been described when the lungs are involved by pseudomonas bacteremia. The severe toxicity and the pathogenesis of the pulmonary lesions in these patients are probably induced by local production and subsequent circulation of elastase, hemolysin, lecithinase, and lipase elaborated by these bacilli.
Evidence for a cross-talk between human neutrophils and Th17 cells
Authors: Martin Pelletier, Laura Maggi, Alessandra Micheletti, Elena Lazzeri, Nicola Tamassia, Claudio Costantini, Lorenzo Cosmi, Claudio Lunardi, Francesco Annunziato, Sergio Romagnani, Marco A. Cassatella
Journal: Blood (2009)
Abstract 2473: Fragment-based discovery of inhibitors of replication protein A protein-protein interactions.
Authors: Alex G. Waterson, James D. Patrone, J. Phillip Kennedy, Nicholas F. Pelz, Andreas O. Frank, Bhavatarini Vandgamudi, Michael D. Feldkamp, Elaine M. Souza-Fagundes, Olivia W. Rossanese, Walter J. Chazin, Stephen W. Fesik
Journal: Cancer Research (2013)

Abstract Replication Protein A (RPA) is a heterotrimeric protein that binds to and protects ssDNA and plays an integral role in initiating the cellular response to DNA damage. This response is mediated via protein-protein interactions between a basic cleft on the RPA70N subunit and a number of protein partners, including ATRIP, Rad9, Mre11, and p53. RNAi against RPA has shown an expected toxicity against cancer cell lines, possibly due to abrogation of the ssDNA binding function of RPA. Specific disruption of the protein-protein interactions between the RPA07N subunit and its binding partners has the potential to produce a more selective cytotoxic response in cancer cells. To more accurately dissect the therapeutic relevance of disrupting only the protein-protein interaction functions of RPA, we sought to discover potent small molecule probes that bind to the basic cleft of RPA70N. Inhibition of protein-protein interactions is considered a difficult task. An NMR-based fragment screen has identified more than 130 fragment molecules that bind to the RPA70N protein-protein interaction cleft with affinities that range from 500 μM to 2 mM and corresponding ligand efficiencies from 0.18 to 0.30. Selected fragments, representing several distinct chemotypes, have been optimized for binding to the protein. Using X-ray crystallography, the binding modes of these fragments have been defined. Fragments were found to bind primarily to two main sites within the basic cleft of RPA70N. Additional structure-guided optimizations have been carried out and ternary co-crystal structures have been generated to guide fragment linking strategies. Together, these activities have led to the creation of multiple lead series of inhibitors of the RPA:ATRIP interaction, with binding affinities improved by several fold over the initial fragments. The SAR and biological activities of the fragments and lead compounds will be discussed. Citation Format: Alex G. Waterson, James D. Patrone, J. Phillip Kennedy, Nicholas F. Pelz, Andreas O. Frank, Bhavatarini Vandgamudi, Michael D. Feldkamp, Elaine M. Souza-Fagundes, Olivia W. Rossanese, Walter J. Chazin, Stephen W. Fesik. Fragment-based discovery of inhibitors of replication protein A protein-protein interactions. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2473. doi:10.1158/1538-7445.AM2013-2473
AAV-Mediated expression of galactocerebrosidase in brain results in attenuated symptoms and extended life span in murine models of globoid cell leukodystrophy
Authors: Mohammad A. Rafi, Han Zhi Rao, Marco A. Passini, Mark Curtis, Marie T. Vanier, Mariam Zaka, Paola Luzi, John H. Wolfe, David A. Wenger
Journal: Molecular Therapy (2005)

Globoid cell leukodystrophy (GLD) or Krabbe disease is a neurodegenerative disorder caused by a deficiency of galactocerebrosidase (GALC) activity. GALC is required for the lysosomal degradation of galactosylceramide, psychosine, and possibly other galactolipids. This process is extremely important during active myelination. In the absence of functional GALC, psychosine accumulates, resulting in the apoptotic death of myelin-producing cells. While most patients are infants who do not survive beyond 2 years of age, some older patients are also diagnosed. Hematopoietic stem cell transplantation has proven to have a positive effect on the course of some patients with late-onset Krabbe disease. Murine models of this disease provide an excellent opportunity to evaluate therapeutic alternatives including gene therapy. In this study we used serotype 1 AAV to express mouse GALC under the control of the human cytomegalovirus promoter. Direct administration of these viral particles into the brains of neonatal mice with GLD resulted in sustained expression of GALC activity, improved myelination, attenuated symptoms, and prolonged life span. While this treatment also resulted in significant pathological improvements, the treated mice died with symptoms similar to those of the untreated mice. Additional initiatives may be required to prevent the onset of disease and reverse the course of the disease in animal models and human patients.
Renal Response to Pyrogen in Normotensive and Hypertensive Man
Authors: ERVIN A. GOMBOS, TAE HUNG LEE, JOSEPH SOLINAS, MIRA MITROVIC
Journal: Circulation (1967)

Pyrogen, when administered intravenously to normotensive and hypertensive men, produced initially vasoconstriction and subsequently marked renal vasodilatation, both resulting from direct effects on the renal vasculature. The absolute renal plasma flow, both during control observations and at the height of renal hyperemia, was greater in normotensives, but the percentage change was greater in hypertensive subjects. Extraction of p -aminohippurate diminished during renal hyperemia. Medullary renal plasma flow was higher in normotensive subjects both during control observations and at the height of renal hyperemia. Simultaneously with the development of renal hyperemia, sodium excretion increased without alterations in filtered load of sodium and was prompt in its development in normotensives and delayed in hypertensives. This is attributed to a transient, small, yet probably significant decrease in filtered load of sodium during vasoconstriction immediately following the administration of pyrogen. Simultaneously with the development of renal hyperemia, there was in both groups an increase in solute excretion and tubular reabsorption of solute-free water.
Abstract 4212: Deep sequencing analysis of the ALK kinase domain identifies resistant mutations in relapse patients following crizotinib treatment.
Authors: Stephen Huang
Journal: Cancer Research (2013)

Abstract ALK resistance mutations conferring resistance to Xalkori vary widely within the tyrosine kinase domain. So far, nine different acquired resistant mutations have been identified in the clinic, including 1151Tins, L1152R, C1156Y, F1174C, L1196M, G1202R, D1203N, S1206Y, and, G1296A. We developed a deep sequencing method using semiconductor sequencing technology to quickly detect resistance mutations within the ALK kinase domain in tumor biopsies. By bar-coding, we were able to sequence multiple samples in a single run. We have also developed an algorithm to determine assay noisy level and call mutations automatically. The assay sensitivity to call close to 1% mutated allele demonstrated by a titration experiment with a cell line harboring a know mutation. Genomic DNA analysis from relapsed patients revealed several known mutations. Our assay demonstrates robust and sensitive detection of ALK kinase mutations in NSCLC tumor samples and aids in the elucidation of resistance mechanisms pertinent to the clinical setting. Citation Format: Stephen Huang. Deep sequencing analysis of the ALK kinase domain identifies resistant mutations in relapse patients following crizotinib treatment. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4212. doi:10.1158/1538-7445.AM2013-4212
Current causes of fixed drug eruption in the U.K.
Authors: J.A. Savin
Journal: British Journal of Dermatology (2001)

Sir, In every country, the causes of fixed drug eruption (FDE) follow the local patterns of drug usage; these change as old drugs drop out of use and are replaced by new ones with an unknown potential for causing this type of reaction. This study is an attempt to establish the current causes of FDE in the U.K. In total, 271 U.K. patients with FDE were traced, of whom 208 had been reported to the Committee on Safety of Medicines (CSM) between January 1991 and December 1999. The other 63 had been seen between October 1997 and October 1999 by members of the British Association of Dermatologists (BAD) who responded to a questionnaire on this topic. The drugs reported most commonly to the CSM in association with an FDE are listed individually in Table 1, and by group in Table 2. The most frequent causes of FDE reported by members of the BAD are shown in Tables 3 and 4.
Gap junction heterogeneity in reentrant ventricular tachycardia
Authors: R VEENSTRA
Journal: Cardiovascular Research (2006)

See article by Cabo et al.  [18]  (pages 241−249) in this issue .

The cells of all tissues are coupled by gap junctions, with the exception of blood and adult skeletal muscle cells. Gap junctions provide for homoeostasis and integrative function by mediating the exchange of ions and chemical second messenger molecules between adjacent cells. The electrical signaling provided by these specialized intercellular junctions is perhaps best understood in the heart, where their abundance serves to provide low electrical resistance pathways for current flow – ensuring fast and efficient transfer of the propagating cardiac action potential throughout the working myocardium. This role of the gap junction, or nexus as it was then called, was best demonstrated by Barr et al. [1] when they correlated the block of cardiac action potential propagation with disruption of the gap junction structure by extracellular hypertonic sucrose solutions [1]. Studies since the 1960s investigated the membrane structure of gap junctions, and the only structural changes noted were associated with irreversible uncoupling procedures [2–4]. These irreversible structural changes occurred after prolonged acidification or mechanical trauma and … 

*Corresponding author. Tel.: +1 315 464 5145; fax: +1 315 464 8014. Email address: veenstrr{at}upstate.edu
A Break for the Coffee-Break
Authors: Samuel Vaisrub
Journal: JAMA (1975)

Ever since its discovery a thousand or so years ago, coffee has been a source of contention. It has brewed antagonisms ranging from religious objections by Orthodox Moslems who regarded it as an intoxicant to imputations of sterilizing and abortifacient effects by some 18th century physicians. A rumored ban on coffee for women provoked Bach into composing his<i>Coffee Cantata</i>. And Ben Jonson called coffee "a loathsome poison—not yet understood." Apparently it is not yet understood even now. While some investigators link coffee with coronary heart disease, others do not. Paul and co-workers<sup>1</sup>reported a positive association between the two. Gould et al<sup>2</sup>drew similar conclusions from their observations. A specific relatedness of myocardial infarction to coffee intake has been reported by others.<sup>3,4</sup> To be sure, coffee provides ample grounds for supporting such conclusions. Caffeine is known to precipitate ectopic beats and to accelerate the heart rate.
Hepatitis C virus infection among Greek renal transplant patients.
Authors: I Vafiadis, J Boletis, H Papastathi, J Delladetsima, C Stathakis, A Hatzakis, A Kostakis, G Vosnides
Journal: Gut (1993)

In this study, the prevalence of hepatitis C virus (HCV) infection among renal transplant recipients was high, directly proportional to the haemodialysis time before transplant and inversely proportional to the time after this. There was evidence of previous infection with hepatitis B virus (HBV), and a high prevalence of abnormal liver function tests. Virus induced chronic hepatitis lesions were rare, probably as a result of immunosuppression.
Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
Authors: Alexander Krüttgen, Hans-Peter Horz, Josefine Weber-Heynemann, Mihael Vucur, Christian Trautwein, Gerhard Haase, Tom Luedde, Christoph Roderburg
Journal: Gut Microbes (2012)

Helicobacter species are important pathogens and previous studies in mice suggested a link between colonization by Helicobacter hepaticus (H. hepaticus) and hepatocellular carcinoma (HCC). This study aimed at corroborating this potential link in human patients. We used a sensitive and specific Helicobacter ssp PCR assay to screen stool samples from a collective of patients with viral-induced HCC (hepatitis B or hepatitis C) and a control group for presence of Helicobacter ssp DNA. Although retrieving DNA of H. pylori and H. canadensis from stool samples of non-HCC patients, we found no evidence indicating the presence of H. hepaticus in HCC-patients with chronic hepatitis B or hepatitis C. Interestingly we found H. canadensis in a stool sample of a patient presenting with diarrhea. Taken together, our data argue against a pathogenic role of H. hepaticus in viral-induced HCC. Yet, our results do not exclude a role of H. hepaticus in those HCC cases caused by other carcinogens, such as aflatoxin. Moreover, we speculate that H. canadensis might be a novel gastrointestinal pathogen.
Specific killing of multiple myeloma cells by (-)-epigallocatechin-3-gallate extracted from green tea: biologic activity and therapeutic implications
Authors: Masood A. Shammas, Paola Neri, Hemanta Koley, Ramesh B. Batchu, Robert C. Bertheau, Vidit Munshi, Rao Prabhala, Mariateresa Fulciniti, Yu tzu Tai, Steven P. Treon, Raj K. Goyal, Kenneth C. Anderson, Nikhil C. Munshi
Journal: Blood (2006)