tbuckley/cabot-search huggingface dataset:
Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays
Authors: Samuel P Hazen, Felix Naef, Tom Quisel, Joshua M Gendron, Huaming Chen, Joseph R Ecker, Justin O Borevitz, Steve A Kay
Journal: Genome biology (2009)

Organisms are able to anticipate changes in the daily environment with an internal oscillator know as the circadian clock. Transcription is an important mechanism in maintaining these oscillations. Here we explore, using whole genome tiling arrays, the extent of rhythmic expression patterns genome-wide, with an unbiased analysis of coding and noncoding regions of the Arabidopsis genome.As in previous studies, we detected a circadian rhythm for approximately 25% of the protein coding genes in the genome. With an unbiased interrogation of the genome, extensive rhythmic introns were detected predominantly in phase with adjacent rhythmic exons, creating a transcript that, if translated, would be expected to produce a truncated protein. In some cases, such as the MYB transcription factor AT2G20400, an intron was found to exhibit a circadian rhythm while the remainder of the transcript was otherwise arrhythmic. In addition to several known noncoding transcripts, including microRNA, trans-acting short interfering RNA, and small nucleolar RNA, greater than one thousand intergenic regions were detected as circadian clock regulated, many of which have no predicted function, either coding or noncoding. Nearly 7% of the protein coding genes produced rhythmic antisense transcripts, often for genes whose sense strand was not similarly rhythmic.This study revealed widespread circadian clock regulation of the Arabidopsis genome extending well beyond the protein coding transcripts measured to date. This suggests a greater level of structural and temporal dynamics than previously known.
Increased prevalence of methylenetetrahydrofolate reductase C677T variant in patients with IBD Reply
Authors: J N NIELSEN
Journal: Gut (2000)

Editor,—We read with interest the paper by Mahmud et al ((1999) Gut 45:389–394; [OpenUrl][1][CrossRef][2][PubMed][3][Web of Science][4]) . The study showed an increased prevalence of methylenetetrahydrofolate reductase (MTHFR) C677T variant in patients with inflammatory bowel disease (IBD). The C677T polymorphism is a known genetic cause of mild hyperhomocysteinaemia (hyper-tHcy)1 and may be associated with a variable degree of risk for thromboembolic disease in patients with IBD.2 To confirm a higher prevalence of the C677T polymorphism, we investigated 99 patients with established IBD for this polymorphism compared with 1084 unselected newborns.3 DNA samples were genotyped for the MTHFR (C677T) mutation. Patients were categorised as homozygous for the thermolabile variant … [1]: {openurl}?query=rft.jtitle%253DNature%2Bgenetics%26rft.stitle%253DNat%2BGenet%26rft.aulast%253DFrosst%26rft.auinit1%253DP.%26rft.volume%253D10%26rft.issue%253D1%26rft.spage%253D111%26rft.epage%253D113%26rft.atitle%253DA%2Bcandidate%2Bgenetic%2Brisk%2Bfactor%2Bfor%2Bvascular%2Bdisease%253A%2Ba%2Bcommon%2Bmutation%2Bin%2Bmethylenetetrahydrofolate%2Breductase.%26rft_id%253Dinfo%253Adoi%252F10.1038%252Fng0595-111%26rft_id%253Dinfo%253Apmid%252F7647779%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [2]: /lookup/external-ref?access_num=10.1038/ng0595-111&link_type=DOI [3]: /lookup/external-ref?access_num=7647779&link_type=MED&atom=%2Fgutjnl%2F47%2F3%2F456.atom [4]: /lookup/external-ref?access_num=A1995QX57300025&link_type=ISI
Confirming Position of Venous Port
Authors: Dt Sleiifer, Nh Mulder, Ege Devries
Journal: Annals of Internal Medicine (1990)

Letters and Corrections1 July 1990Confirming Position of Venous PortD.Th. Sleijfer, MD, N.H. Mulder, MD, E.G.E. de Vries, MDD.Th. Sleijfer, MDSearch for more papers by this author, N.H. Mulder, MDSearch for more papers by this author, E.G.E. de Vries, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-113-1-86_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editors: Totally implanted subcutaneous venous access ports are now widely used in patients treated with cancer chemotherapy. Complications associated with the use of these ports include local extravasation of the chemotherapeutic agents and migration of the catheter, also leading to extravasation. The intravascular position of the catheter before administration of chemotherapy can be verified by withdrawing blood through the port. In some patients, however, withdrawal fails. In these patients, fluoroscopy can be done during injection of a contrast medium into the port.In the past, in patients with heart failure, circulation time was determined by injecting a substance... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: University Hospital 9713 EZ Groningen, the Netherlands PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 July 1990Volume 113, Issue 1Page: 86-86KeywordsBloodCancer chemotherapyCathetersChemotherapeutic agentsChemotherapyHeart failure ePublished: 1 December 2008 Issue Published: 1 July 1990 PDF downloadLoading ...
Radiofrequency catheter ablation of ventricular tachycardia in patients without structural heart disease.
Authors: L S Klein, H T Shih, F K Hackett, D P Zipes, W M Miles
Journal: Circulation (1992)

BACKGROUND Radiofrequency energy has been used safely and successfully to eliminate accessory pathways in patients with the Wolff-Parkinson-White syndrome and the substrate for atrioventricular nodal reentrant tachycardia. However, this form of ablation has had only limited success in eliminating ventricular tachycardia in patients with structural heart disease. In contrast, direct-current catheter ablation has been used successfully to eliminate ventricular tachycardia in patients with and without structural heart disease. The purpose of this study was to test whether radiofrequency energy can safely and effectively ablate ventricular tachycardia in patients without structural heart disease. METHODS AND RESULTS Sixteen patients (nine women and seven men; mean age, 38 years; range, 18-55 years) without structural heart disease who had ventricular tachycardia underwent radiofrequency catheter ablation to eliminate the ventricular tachycardia. Two patients presented with syncope, nine with presyncope, and five with palpitations only. Mean duration of symptoms was 6.7 years (range, 0.5-20 years). Radiofrequency catheter ablation successfully eliminated ventricular tachycardia in 15 of 16 patients (94%). Sites of ventricular tachycardia origin included the high right ventricular outflow tract (12 patients), the right ventricular septum near the tricuspid valve (three patients), and the left ventricular septum (one patient). The only ablation failure was in a patient whose ventricular tachycardia arose from a region near the His bundle. An accurate pace map, early local endocardial activation, and firm catheter contact with endocardium were associated with successful ablation. Radiofrequency ablation did not cause arrhythmias, produced minimal cardiac enzyme rise, and resulted in no detectable change in cardiac function by Doppler echocardiography. CONCLUSIONS Radiofrequency catheter ablation of ventricular tachycardia in patients without structural heart disease is effective and safe and may be considered as early therapy in these patients.
Antibacterial mechanisms of the urinary bladder
Authors: Carl W. Norden, Gareth M. Green, Edward H. Kass
Journal: Journal of Clinical Investigation (1968)

The disappearance of bacteria from the normal urinary bladder is apparently a function of two host defense mechanisms: the mechanical clearance of organisms by voiding, and the antibacterial activity of the bladder wall. This study quantified the relative contribution of each of these mechanisms to the resistance of the bladder to bacterial infection.
Remarks on the Choice of Mechanical Treatment in Spinal Disease: With Remarks on After-Treatment and Prognosis
Authors: C. H. Golding-Bird
Journal: BMJ (1882)

GiVEN a case of spinal disease; is any mechanical treatment to be adopted; and, if so, what form is it to take?This is a most common question, the answer to which must mainly come from the practitioner's general experience of the diseases which deform the spine; yet rules may be formulated to serve as a broad guide in most cases.Omitting mention of drugs or of operation, e.g-., opening of abscesses,.and confining the remarks now to be offered to cases of caries and of general curvature, the following rules or guides for a choice of me- chanical treatment may be laid down.No description of treatment is offered.The old mechanical appliances or "spinals", and their use, will be found in works on orthoptedic surgery.Sayre's method is de- scribed in his book on Spinal Cuirvature, and in the BRITrisH MEDICAL
Association of Kidney Function with Incident Hip Fracture in Older Adults
Authors: Linda F. Fried, Mary Louise Biggs, Michael G. Shlipak, Stephen Seliger, Bryan Kestenbaum, Catherine Stehman-Breen, Mark Sarnak, David Siscovick, Tamara Harris, Jane Cauley, Anne B. Newman, John Robbins
Journal: Journal of the American Society of Nephrology (2006)

The effect of loperamide and naloxone on mouth-to-caecum transit time was evaluated by the lactulose hydrogen breath test in four men and four women. Each subject underwent tests during the administration of placebo, loperamide (12-16 mg po), naloxone (40 micrograms/kg/h by a three-hour intravenous infusion), and loperamide plus naloxone, carried out at intervals of one or two weeks. The transit time was significantly longer after loperamide, and this effect was antagonised by the concomitant administration of naloxone whereas naloxone administered alone had no effect on mean transit time. No clinically important side effects were reported.
Screening for small for dates fetuses: a controlled trial.
Authors: J P Neilson, S P Munjanja, C R Whitfield
Journal: BMJ (1984)

In the hope of reducing perinatal risks associated with retardation of intrauterine growth a previously described two stage ultrasound screening schedule was evaluated by a controlled trial in 877 women with low risk single pregnancies. The two stages of ultrasound examination were an assessment of gestational age during early pregnancy followed by measurement of length from crown to rump and area of trunk at between 34 and 36 weeks9 gestation. The product of crown to rump length and trunk area was calculated. The sensitivity of this schedule in identifying in advance 94% of babies who were small for dates at birth, with 90% specificity, and the speed and simplicity of measurement confirmed the accuracy and feasibility of two stage ultrasonography as a screening procedure. The controlled trial did not, however, show any benefit from its routine application in these low risk pregnancies.
Case 34-1990
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, William F. McNeely, Betty U. McNeely, W.H. Harris, A.E. Rosenberg
Journal: New England Journal of Medicine (1990)

Case Records of the Massachusetts General Hospital from The New England Journal of Medicine — Case 34-1990 — A 74-Year-Old Man with a Failed Right-Hip Replacement, Constant Pain Centered in the Right Hip, and an Osteolytic Lesion in the Right Acetabulum
Changes in intestinal alkaline phosphatase activity in cholera toxin-treated rats.
Authors: S Miura, H Asakura, T Morishita, T Hibi, Y Munakata, K Kobayashi, M Tsuchiya
Journal: Gut (1982)

It is conceivable that brush border enzyme activities of the intestinal mucosa will change when bacterial toxins are exposed to the intestinal microvillous membranes. The effect of cholera toxin on the activity of intestinal alkaline phosphatase in rats was therefore determined in the intestinal mucosa by the histochemical method as well as in intestinal lymph by using lymph fistulated-rats. Activity of intestinal alkaline phosphatase in the intestinal mucosa and lymphatics changed biphasically after the oral administration of cholera toxin to rats. For the first three hours after the administration of cholera toxin it was depressed; it then increased and at eight hours reached a maximum. These changes in the activity of intestinal alkaline phosphatase were prevented by the administration of chlorpromazine, a known inhibitor of adenylate cyclase activity.
Endothelial epsin deficiency decreases tumor growth by enhancing VEGF signaling
Authors: Satish Pasula, Xiaofeng Cai, Yunzhou Dong, Mirko Messa, John McManus, Baojun Chang, Xiaolei Liu, Hua Zhu, Robert Silasi Mansat, Seon-Joo Yoon, Scott Hahn, Jacob Keeling, Debra Saunders, Genevieve Ko, John Knight, Gail Newton, Francis Luscinskas, Xiaohong Sun, Rheal Towner, Florea Lupu, Lijun Xia, Ottavio Cremona, Pietro De Camilli, Wang Min, Hong Chen
Journal: Journal of Clinical Investigation (2012)

Epsins are a family of ubiquitin-binding, endocytic clathrin adaptors. Mice lacking both epsins 1 and 2 (Epn1/2) die at embryonic day 10 and exhibit an abnormal vascular phenotype. To examine the angiogenic role of endothelial epsins, we generated mice with constitutive or inducible deletion of Epn1/2 in vascular endothelium. These mice exhibited no abnormal phenotypes under normal conditions, suggesting that lack of endothelial epsins 1 and 2 did not affect normal blood vessels. In tumors, however, loss of epsins 1 and 2 resulted in disorganized vasculature, significantly increased vascular permeability, and markedly retarded tumor growth. Mechanistically, we show that VEGF promoted binding of epsin to ubiquitinated VEGFR2. Loss of epsins 1 and 2 specifically impaired endocytosis and degradation of VEGFR2, which resulted in excessive VEGF signaling that compromised tumor vascular function by exacerbating nonproductive leaky angiogenesis. This suggests that tumor vasculature requires a balance in VEGF signaling to provide sufficient productive angiogenesis for tumor development and that endothelial epsins 1 and 2 negatively regulate the output of VEGF signaling. Promotion of excessive VEGF signaling within tumors via a block of epsin 1 and 2 function may represent a strategy to prevent normal angiogenesis in cancer patients who are resistant to anti-VEGF therapies.
Regulatory T cells in rheumatoid arthritis showed increased plasticity toward Th17 but retained suppressive function in peripheral blood
Authors: Tian Wang, Xiaolin Sun, Jing Zhao, Jing Zhang, Huaqun Zhu, Chun Li, Na Gao, Yuan Jia, Dakang Xu, Fang-Ping Huang, Ningli Li, Liwei Lu, Zhan-Guo Li
Journal: Annals of the Rheumatic Diseases (2014)

Regulatory T cells (Tregs) with the plasticity of producing proinflammatory cytokine IL-17 have been demonstrated under normal and pathogenic conditions. However, it remains unclear whether IL-17-producing Tregs lose their suppressive functions because of their plasticity toward Th17 in autoimmunity. The aim of this study was to investigate IL-17-producing Tregs from patients with rheumatoid arthritis (RA), and characterise their regulatory capacity and clinical significance.Foxp3 and IL-17 coexpression were evaluated in CD4 T lymphocytes from RA patients. An in vitro T cell polarisation assay was performed to investigate the role of proinflammatory cytokines in IL-17-producing Treg polarisation. The suppressive function of IL-17-producing Tregs in RA was assessed by an in vitro suppression assay. The relationship between this Treg subset and clinical features in RA patients was analysed using Spearman's rank correlation test.A higher frequency of IL-17-producing Tregs was present in the peripheral blood of RA patients compared with healthy subjects. These cells from peripheral blood showed phenotypic characteristics of Th17 and Treg cells, and suppressed T cell proliferation in vitro. Tregs in RA synovial fluid lost suppressive function. The Th17 plasticity of Tregs could be induced by IL-6 and IL-23. An increased ratio of this Treg subset was associated with decreased levels of inflammatory markers, including the erythrocyte sedimentation rate and C-reactive protein level, in patients with RA.Increased levels of IL-17-producing Tregs were identified in RA patients. This Treg subset with Th17 plasticity in peripheral blood retained suppressive functions and was associated with milder inflammatory conditions, suggesting that this Treg population works as a negative regulator in RA, but in RA synovial site it may be pathogenic.
Pharmacological responsiveness of the myoepithelium of the isolated human axillary apocrine sweat gland
Authors: KENZO SATO
Journal: British Journal of Dermatology (1980)

Human axillary apocrine glands are endowed with well developed myoepithelium, but its pharmacological responsiveness has remained unknown. The present study is part of our efforts in determining the role(s) played by exocrine myoepithelium in the overall secretory processes. Single human apocrine glands were isolated from biopsy skin specimens and were stimulated with various pharmacological agents in vitro. Tubular contraction was recorded photomicrographically and was interpreted as being due to myoepithelial contraction. The tubular contraction was induced by stimulation with phenylephrine (5 X 10(-6)M) or adrenaline (5 X 10(-6)M) but not with isoproterenol (5 X 10(-6)M) or acetylcholine (5 X 10(-6)M). A higher concentration of acetylcholine (10(-4)M) produced a minor degree of contraction. The phenylephrine-induced contraction was blocked by phentolamine (10(-5)M) or by EGTA (5 mM) but not by propranolol (10(-5)M) or by atropine (10(-5)M). It was concluded that the contractile response of the apocrine myoepithelium is selectively controlled by the alpha-adrenergic stimulation. The possibility is discussed that the major function of the apocrine myoepithelium is not just the pumping out of the preformed sweat but is, as in the eccrine sweat gland myoepithelium, the provision of structural support for the apocrine secretory epithelium to withstand the increase, if any, in the luminal hydrostatic pressure during apocrine sweat secretion.
Chemotherapy Versus Autologous Stem-Cell Transplantation for the Treatment of Transformed Follicular Lymphoma in the Rituximab Era
Authors: Constantine S. Tam, Alan Herschtal, Michael Dickinson, Anupkumar George, John F. Seymour
Journal: Journal of Clinical Oncology (2013)

to benefit from the addition of systemic agents because of the already high local control of radiosurgery.As such, we chose to focus on patients with multiple CNS metastases, for whom WBRT is considered standard treatment and might be improved by the addition of a radiosensitizing agent such as erlotinib.Our hope was, and is, to improve the therapeutic ratio in a setting in which dose-escalation is not feasible because of the risk of neurotoxicity.
Maturation in vitro of immature human oocytes for clinical use
Authors: K-Y Cha
Journal: Human Reproduction Update (1998)

Human oocyte maturation is considered as the reinitiation and completion of the first meiotic division from the germinal vesicle stage (prophase I) to metaphase II, and the accompanying cytoplasmic maturation for fertilization and early embryonic development. Immature human oocytes obtained from patients undergoing gynaecological surgery, or ovulation induction or having polycystic ovary syndrome (PCOS) can be matured and fertilized in vitro. To date, 80% of immature oocytes matured to metaphase II when cultured in maturation medium supplemented with gonadotrophins and 85% of matured oocytes fertilized and cleaved in vitro. Following transfer of these embryos, pregnancies and live births have been achieved. However, the capacity for oocyte maturation was different when the immature oocytes were retrieved from PCOS patients and when the oocytes were cryopreserved at germinal vesicle stage. Keywords:fertilization/germinal vesicle/human/maturation/oocyte
Novel Matrix Metalloproteinase Inhibitor [18F]Marimastat-Aryltrifluoroborate as a Probe for <i>In vivo</i> Positron Emission Tomography Imaging in Cancer
Authors: Ulrich auf dem Keller, Caroline L. Bellac, Ying Li, Yuanmei Lou, Philipp F. Lange, Richard Ting, Curtis Harwig, Reinhild Kappelhoff, Shoukat Dedhar, Michael J. Adam, Thomas J. Ruth, François Bénard, David M. Perrin, Christopher M. Overall
Journal: Cancer Research (2010)

Matrix metalloproteinases (MMP), strongly associated pathogenic markers of cancer, have undergone extensive drug development programs. Marimastat, a noncovalent MMP inhibitor, was conjugated with FITC to label cellular metalloproteinase cancer targets in MDA-MB-231 cells in vitro. Punctate localization of active transmembrane MMP14 was observed. For molecular-targeted positron emission tomography imaging of syngeneic 67NR murine mammary carcinoma in vivo, marimastat was (18)F-labeled using a shelf-stable arylboronic ester conjugate as a captor for aqueous [(18)F]fluoride in a novel, rapid one-step reaction at ambient temperature. [(18)F]Marimastat-aryltrifluoroborate localized to the tumors, with labeling being blocked in control animals first loaded with >10-fold excess unlabeled marimastat. The labeled drug cleared primarily via the hepatobiliary and gastrointestinal tract, with multiple animals imaged in independent experiments, confirming the ease of this new labeling strategy.
Desquamative alveolar disease (desquamative interstitial pneumonia): case report
Authors: E. Cruz, J. Rodriguez, C. Lisboa, R. Ferretti
Journal: Thorax (1969)

Desquamative interstitial pneumonia is a disease characterized by massive alveolar cell proliferation and desquamation with sparse interstitial involvement. The reported case shows an unusually widespread radiographic reticulo-nodular image and abundant alveolar cells in the sputum. Functional studies reveal the expected diffusion defect with practically normal mechanical properties of the lung, in contrast with interstitial fibrosing lung diseases. On the basis of the pathological findings, especially the behaviour of alveolar cells, the individuality of this disease is discussed. We think that it is different from other diseases classed as varieties of a single disease or as different entities under the names of primary interstitial fibrosis or chronic fibrosing alveolitis.