Progress in the Development of Hepatitis C Virus Vaccines
Authors: Hildegund CJ Ertl
Journal: Molecular Therapy (2012)

Vaccines save millions of lives each year. Although vaccines are available for many of the viral infections that can be readily prevented by neutralizing antibodies, vaccines for more complex pathogens—including viruses that mutate very rapidly and may require induction of broadly cross-reactive cellular immune responses—remain elusive. Two recent articles1Colloca S Barnes E Folgori A Ammendola V Capone S Cirillo A et al.Vaccine vectors derived from a large collection of simian adenoviruses induce potent cellular immunity across multiple species.Sci Transl Med. 2012; 4: 115ra2Crossref PubMed Scopus (227) Google Scholar,2Barnes E Folgori A Capone S Swadling L Aston S Kurioka A et al.Novel adenovirus-based vaccines induce broad and sustained T cell responses to HCV in man.Sci Transl Med. 2012; 4: 115ra1Crossref PubMed Scopus (332) Google Scholar report on vaccine vectors derived from adenoviruses (Ads) of three different species isolated from chimpanzee feces. Vectors encoding antigens from expression cassettes placed into the deleted E1 domain were found to be highly immunogenic in mice and monkeys.1Colloca S Barnes E Folgori A Ammendola V Capone S Cirillo A et al.Vaccine vectors derived from a large collection of simian adenoviruses induce potent cellular immunity across multiple species.Sci Transl Med. 2012; 4: 115ra2Crossref PubMed Scopus (227) Google Scholar Even more important—because mice can lie and monkeys can exaggerate—vectors expressing the NS3-5B region of hepatitis C virus (HCV) genotype 1B induced potent and sustained transgene product–specific CD8+ T-cell responses in human volunteers.2Barnes E Folgori A Capone S Swadling L Aston S Kurioka A et al.Novel adenovirus-based vaccines induce broad and sustained T cell responses to HCV in man.Sci Transl Med. 2012; 4: 115ra1Crossref PubMed Scopus (332) Google Scholar Traditional vaccines are based on inactivated or attenuated pathogens, purified proteins, or modified toxins. Cellular immunity, especially CD8+ T-cell responses, can best be achieved by gene transfer vehicles that induce de novo synthesis of the vaccine antigens, which are in part cleaved by the proteasome in the cytoplasm. Peptides derived from the degraded antigens are actively transported into the endoplasmic reticulum, where they associate with major histocompatibility class I antigen, and then undergo translocation to the cell surface, where they can interact with the T-cell receptors on CD8+ cells. More than 15 years ago, replication-defective human serotype 5 adenovirus (AdHu5) vectors originally developed by gene therapists for correction of genetic defects were shown to be potent inducers of B and CD8+ T cell–mediated immunity.3Xiang ZQ Yang Y Wilson JM Ertl HC A replication-defective human adenovirus recombinant serves as a highly efficacious vaccine carrier.Virology. 1996; 219: 220-227Crossref PubMed Scopus (225) Google Scholar Not only are AdHu5 vectors far more immunogenic than other genetic vaccine carriers such as poxvirus or plasmid vectors, they also induce remarkably more sustained immune responses, most likely because of the vectors’ low-level persistence in a transcriptionally active form in T cells.4Tatsis N Fitzgerald JC Reyes-Sandoval A Harris-McCoy KC Hensley SE Zhou D et al.Adenoviral vectors persist in vivo and maintain activated CD8+ T cells: implications for their use as vaccines.Blood. 2007; 110: 1916-1923Crossref PubMed Scopus (171) Google Scholar Nevertheless, as had already been appreciated by gene therapists and was then confirmed in vaccine studies, preexisting AdHu5-specific neutralizing antibodies, which are very common in humans, reduce the vectors’ transduction rates and hence expression of the transgene products.5Zhi Y Figueredo J Kobinger GP Hagan H Calcedo R Miller JR et al.Efficacy of severe acute respiratory syndrome vaccine based on a nonhuman primate adenovirus in the presence of immunity against human adenovirus.Hum Gene Ther. 2006; 17: 500-506Crossref PubMed Scopus (47) Google Scholar More than 10 years ago, E1-deleted vectors derived from chimpanzee Ad viruses (S-AdV or AdC) were developed to circumvent preexisting neutralizing antibodies in humans,5Zhi Y Figueredo J Kobinger GP Hagan H Calcedo R Miller JR et al.Efficacy of severe acute respiratory syndrome vaccine based on a nonhuman primate adenovirus in the presence of immunity against human adenovirus.Hum Gene Ther. 2006; 17: 500-506Crossref PubMed Scopus (47) Google Scholar and since then a steady stream of publications has shown that AdC vectors are highly immunogenic in mice and nonhuman primates (reviewed in refs. 6Tatsis N Tesema L Robinson ER Giles-Davis W McCoy K Gao GP et al.Chimpanzee-origin adenovirus vectors as vaccine carriers.Gene Ther. 2006; 13: 421-429Crossref PubMed Scopus (80) Google Scholar and 7Lasaro MO Ertl HC New insights on adenovirus as vaccine vectors.Mol Ther. 2009; 17: 1333-1339Abstract Full Text Full Text PDF PubMed Scopus (206) Google Scholar). The recent article by Colloca et al.1Colloca S Barnes E Folgori A Ammendola V Capone S Cirillo A et al.Vaccine vectors derived from a large collection of simian adenoviruses induce potent cellular immunity across multiple species.Sci Transl Med. 2012; 4: 115ra2Crossref PubMed Scopus (227) Google Scholar is therefore not highly innovative, as it confirms a wealth of previous studies. Nevertheless, it is praiseworthy in its breadth by isolating and sequencing in excess of 1,000 Ad viruses from monkey feces. Clearly, any Ad vector used as a vaccine carrier for a given pathogen will induce, in addition to the desired vaccine antigen-specific immune response, carrier-specific neutralizing antibody responses, which will render vaccines based on the same Ad virus inefficient for subsequent use in that target population—whether it be for boosting or the induction of responses to other pathogens. Generation of new simian Ad vectors in addition to those already available8Roy S Medina-Jaszek A Wilson MJ Sandhu A Calcedo R Lin J et al.Creation of a panel of vectors based on ape adenovirus isolates.J Gene Med. 2011; 13: 17-25Crossref PubMed Scopus (27) Google Scholar is thus of value. However, some of the reported findings should be viewed with caution. Relative immunogenicity of vectors was determined using HIV-1 Gag as the transgene product; in our experience with a more limited number of human and simian serotype Ad vectors,5Zhi Y Figueredo J Kobinger GP Hagan H Calcedo R Miller JR et al.Efficacy of severe acute respiratory syndrome vaccine based on a nonhuman primate adenovirus in the presence of immunity against human adenovirus.Hum Gene Ther. 2006; 17: 500-506Crossref PubMed Scopus (47) Google Scholar,7Lasaro MO Ertl HC New insights on adenovirus as vaccine vectors.Mol Ther. 2009; 17: 1333-1339Abstract Full Text Full Text PDF PubMed Scopus (206) Google Scholar immunogenicity of an individual Ad vaccine carrier is in part dictated by the nature of the transgene as well as by characteristics of the expression cassette, such as the presence of introns or enhancers, which may explain discrepancies between Colloca and colleagues’ results and those of earlier studies. For example, both AdHu26, a D family member of the family Adenoviridae,9Liu J Ewald BA Lynch DM Denholtz M Abbink P Lemckert AA et al.Magnitude and phenotype of cellular immune responses elicited by recombinant adenovirus vectors and heterologous prime-boost regimens in rhesus monkeys.J Virol. 2008; 82: 4844-4852Crossref PubMed Scopus (106) Google Scholar and SAd-V23 (also called AdC6), an E family member, have previously been reported to be just as immunogenic in mice and rhesus macaques as AdHu5 vectors.10Tatsis N Lasaro MO Lin SW Haut LH Xiang ZQ Zhou D et al.Adenovirus vector-induced immune responses in nonhuman primates: responses to prime boost regimens.J Immunol. 2009; 182: 6587-6599Crossref PubMed Scopus (46) Google Scholar Rare human as well as nonhuman serotype Ad vectors were originally developed to overcome preexisting neutralizing antibodies in humans. Prevalence rates of such neutralizing antibodies vary depending on the geographical region; prevalence rates are markedly higher in humans residing in developing countries—especially in sub-Saharan Africa—than in individuals from the United States or Europe.11Xiang Z Li Y Cun A Yang W Ellenberg S Switzer WM et al.Chimpanzee adenovirus antibodies in humans, sub-Saharan Africa.Emerg Infect Dis. 2006; 12: 1596-1599Crossref PubMed Scopus (137) Google Scholar,12Chen H Xiang ZQ Li Y Kurupati RK Jia B Bian A et al.Adenovirus-based vaccines: comparison of vectors from three species of adenoviridae.J Virol. 2010; 84: 10522-10532Crossref PubMed Scopus (108) Google Scholar In addition, as was shown in animals5Zhi Y Figueredo J Kobinger GP Hagan H Calcedo R Miller JR et al.Efficacy of severe acute respiratory syndrome vaccine based on a nonhuman primate adenovirus in the presence of immunity against human adenovirus.Hum Gene Ther. 2006; 17: 500-506Crossref PubMed Scopus (47) Google Scholar and then in human volunteers participating in the ill-fated STEP trial,13Buchbinder SP Mehrotra DV Duerr A Fitzgerald DW Mogg R Li D et al.Step Study Protocol Team. Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial.Lancet. 2008; 372: 1881-1893Abstract Full Text Full Text PDF PubMed Scopus (1391) Google Scholar which aimed to test the efficacy of an AdHu5 vector for prevention of chronic HIV-1 infections, titers of Ad-neutralizing antibodies at or above 1:40 or 1:18, respectively, impact the performance of Ad vector vaccines. Colloca et al. tested seroprevalence rates in Caucasians residing in the United States or Europe and report frequencies of neutralizing antibodies to the different Ad viruses above 1:200, which may paint an unduly optimistic picture of the true value of the different AdC viruses as vaccine carriers for use in humans. Barnes et al.2Barnes E Folgori A Capone S Swadling L Aston S Kurioka A et al.Novel adenovirus-based vaccines induce broad and sustained T cell responses to HCV in man.Sci Transl Med. 2012; 4: 115ra1Crossref PubMed Scopus (332) Google Scholar described the immunogenicity of AdHu6 (Ad6) and AdC3 (ChAd3 or SAd-V3) expressing NS3-5B of HCV in a phase I clinical trial. Vectors were used at different doses individually or in a prime-boost regimen. Both vectors induced measurable T-cell responses; at low vector doses, frequencies of vaccine-induced T cells were higher upon AdHu6 immunization. Responses were, as expected from animal studies,10Tatsis N Lasaro MO Lin SW Haut LH Xiang ZQ Zhou D et al.Adenovirus vector-induced immune responses in nonhuman primates: responses to prime boost regimens.J Immunol. 2009; 182: 6587-6599Crossref PubMed Scopus (46) Google Scholar dominated by T cells producing interferon-γ alone or in combination with tumor necrosis factor-α. They showed broad cross-reactivity between different genotypes of HCV and remained largely activated, as indicated by sustained levels of PD-1 and granzyme, limited expression of CD127, and marginal in vivo expansion upon booster immunization with a heterologous Ad vector. Ad vectors, initially hailed as perfect gene delivery vehicles for correction of inherited diseases, in the end failed to achieve sustained gene therapy because of their high immunogenicity. Their subsequent claim to fame as vaccine delivery vehicles was called into question in the aftermath of the STEP trial. The studies by Colloca and Barnes and their colleagues reconfirm that Ad vectors, especially those to which humans lack neutralizing antibodies, should continue to be explored as vaccine carriers for complex pathogens that cannot be thwarted by traditional vaccines.