tbuckley/cabot-search huggingface dataset:
Abstract 2414: In vitro profiling of gastric and liver cancer cell lines developed from Asian cancer patients
Authors: Gerhard Kelter, Armin Maier, Rebekka Krumbach, Julia Schüler, Heinz-Herbert Fiebig, Thomas Beckers
Journal: Cancer Research (2011)

Abstract In East Asia stomach and liver cancer are the 2nd and 3rd most common malignancies and there is a high medical need to develop new drugs. However, well characterized in vitro and in vivo models developed from Asian cancer patients are rare. The Oncotest tumor xenograft and cell line repository used for profiling of anticancer compounds comprised mainly models established from patients with a Caucasian origin. Now the Oncotest tumor repository was extended by 8 gastric and 12 liver cancer cell lines of Asian origin obtained from KCLB (Korean Cell Line Bank) and JCRB (Japanese Collection of Research Bioresources). Additionally, 2 head &amp; neck cancer cell lines from KCLB and the gastric cell line GXA 3011L established at Oncotest from a Korean patient-derived tumor xenograft were added to the panel. Among these 23 cell lines, 6 currently grow subcutaneously as solid tumor xenografts in nude mice. Gene expression profiles (Affymetrix chip HG-U133 plus 2.0 consisting of 38,500 genes) have been determined for all cell lines and respective xenografts. Doubling times as determined for all cell lines were in the range of 23 h (MKN45) to 76 h (SNU-423). In vitro chemosensitivity to standard of care (e.g. 5-FU, doxorubicin, oxaliplatin, paclitaxel) and selected targeted drugs (everolimus, lapatinib) was assessed by a fluorescence based monolayer cytotoxicity assay as well as by the ex-vivo clonogenic assay. The drugs showed diverse patterns of selectivity and potency in both assays. As an example, the mTor inhibitor everolimus exhibited pronounced activity with IC50 values in the low nanomolar range towards the liver cancer cell lines SNU-398, SNU-475, SNU-878 and SNU-886 in the monolayer as well as in the clonogenic assay. In contrast, towards gastric cancer cell lines no or only marginal activity was detected for everolimus in the monolayer assay. The HER2 / EGFR kinase selective inhibitor lapatinib showed selective activity towards the gastric cancer cell line NUGC-4 (IC50=0.16µM), the liver cancer cell line JHH-4 (IC50=0.56µM) and the H&amp;N cancer cell line SNU-1076 (IC50=0.54µM). Overall, in vitro activity profiles for 12 SOC drugs and correlations with molecular alterations, target expression and pathway activation will be presented. This collection of cell lines is of high relevance for the development of drugs against the most widely occurring cancers in East Asia, by providing models for the study of the biology and response to novel anticancer drugs. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2414. doi:10.1158/1538-7445.AM2011-2414
100 A prospective, randomized controlled pilot study of ursodeoxycholic acid (UDCA) combined with mycophenolate mofetil (MMF) in the treatment of primary sclerosing cholangitis
Authors: R STERLING, J SALVATORI, V LUKETIC, A SANYAL, R STRAVITZ, A FULCHER, M CONTOS, A MILLS, M SHIFFMAN
Journal: Hepatology (2003)

Equilibrium vapour pressures of six saturated and seventy-four mono-unsaturated C10-C16 even-carbon aldehydes were determined using a method based on gas chromatographic (GC) retention data. GC measurements were carried out on a 3-m HP-1 capillary column at five to seven temperatures in the range of 35–140 °C by utilising saturated n-12,n-13, n-17 and n-18 hydrocarbons as vapour pressure reference standards. A close correlation, viz. In P [Pa] = 14.008 - 1.105nc (r = 0.99%) has been found between the vapour pressures P and the number of carbon atoms (nc), indicating a vapour pressure decrease by a factor of 3.02 for each CH2 group added in the alkanal chain. For structurally similar subseries of alkenals (ω−3, ω−5 and ω−7 unsaturation), the vapour pressures were also shown to have a simple dependence on nc. Within each alkenal subseries, isomers having Z-configuration had mostly greater vapour pressures than their E-counterparts.
PITYRIASIS ROSEA: REPORT OF A CASE WITH LESIONS OF THE PALMS AND SOLES.*
Authors: FRANK C. COMBES
Journal: British Journal of Dermatology (1932)

Journal Article PITYRIASIS ROSEA: REPORT OF A CASE WITH LESIONS OF THE PALMS AND SOLES. Get access FRANK C. COMBES, M.D. FRANK C. COMBES, M.D. Clinical Professor of Dermatology and Syphilology, University and Bellevue Hospital Medical College, New York Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology and Syphilis, Volume 44, Issue 12, 1 December 1932, Pages 586–587, https://doi.org/10.1111/j.1365-2133.1932.tb09564.x Published: 01 December 1932
Rituximab–cyclophosphamide–dexamethasone combination in the management of autoimmune cytopenias associated with chronic lymphocytic leukemia
Authors: J Rossignol, A-S Michallet, L Oberic, M Picard, A Garon, C Willekens, R Dulery, X Leleu, B Cazin, L Ysebaert
Journal: Leukemia (2010)
A SEASONAL TIDE OF BLOOD PHOSPHATE IN INFANTS
Authors: ALFRED F. HESS
Journal: JAMA (1922)

An investigation<sup>1</sup>undertaken somewhat more than a year ago showed that in infants the inorganic phosphate of the blood could be raised to the normal level by means of frequent exposures to the sun's rays. It was shown later<sup>2</sup>that the same result can be accompushed by irradiation with the carbon arc lamp. It seemed probable that this striking phenomenon—chemical equilibrium brought about by the action of light— is due to the ultraviolet, rather than to the visible rays of the spectrum. As a matter of fact, subsequent investigation has confirmed the view that some ultraviolet radiations possess this biologic action, for when they are filtered out from the spectrum the rays lose this power.<sup>3</sup>As is well known, the composition of the rays of the sun is not at all times the same. There is a marked seasonal variation in its spectrum—in the temperate zone
On Being Observed
Authors: Gregory E. Holt
Journal: Annals of Internal Medicine (2011)

On Being a Doctor16 August 2011On Being ObservedGregory E. Holt, MD, PhDGregory E. Holt, MD, PhDFrom University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-155-4-201108160-00014 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail I thought I had become proficient at advanced cardiac life support (ACLS) by the time I reached my second year of internal medicine residency. Leaning toward entering pulmonary and critical care medicine as a subspecialty, I had learned the algorithms with interest and eschewed the handy reference cards that often crowded the already full pockets of our white coats. I felt so adept and confident in my position that I would sprint to “codes” so I could get there first and run them.During one code on a patient with ventricular fibrillation, we exhausted the initial parts of the algorithm ... Author, Article, and Disclosure InformationAffiliations: From University of Miami Leonard M. Miller School of Medicine, Miami, FL 33136.Corresponding Author: Gregory E. Holt, MD, PhD, Division of Pulmonary, Critical Care, and Sleep Medicine, Leonard M. Miller School of Medicine, P.O. Box 016960 (R-47), Miami, FL 33136; e-mail, [email protected]miami.edu. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 16 August 2011Volume 155, Issue 4Page: 272KeywordsAlgorithmsCritical careHospital medicineNursesSleepStable coronary artery diseaseSub-specialty careWalking ePublished: 16 August 2011 Issue Published: 16 August 2011 Copyright & PermissionsCopyright © 2011 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo
Authors: Sophie Guia, Céline Cognet, Ludovic de Beaucoudrey, Marlowe S. Tessmer, Emmanuelle Jouanguy, Claire Berger, Orchidée Filipe-Santos, Jacqueline Feinberg, Yildiz Camcioglu, Jacob Levy, Suliman Al Jumaah, Sami Al-Hajjar, Jean-Louis Stephan, Claire Fieschi, Laurent Abel, Laurent Brossay, Jean-Laurent Casanova, Eric Vivier
Journal: Blood (2008)
Ketoacidosis during a Low-Carbohydrate Diet
Authors: Pankaj Shah, William L Isley
Journal: New England Journal of Medicine (2006)

To the Editor: It is believed that low-carbohydrate diets work best in reducing weight when producing ketosis.1 We report on a 51-year-old white woman who does not have diabetes but had ketoacidosis while consuming a “no-carbohydrate” diet. There was no family history of diabetes, and she was not currently taking any medications. While adhering to a regimen of carbohydrate restriction, she reached a stable weight of 59.1 kg, a decrease from 72.7 kg. After several months of stable weight, she was admitted to the hospital four times with vomiting but without abdominal pain. On each occasion, she reported no alcohol . . .
Elastin Haploinsufficiency Results in Progressive Aortic Valve Malformation and Latent Valve Disease in a Mouse Model
Authors: Robert B. Hinton, Jennifer Adelman-Brown, Sandra Witt, Varun K. Krishnamurthy, Hanna Osinska, Bhuvaneswari Sakthivel, Jeanne F. James, Dean Y. Li, Daria A. Narmoneva, Robert P. Mecham, D. Woodrow Benson
Journal: Circulation Research (2010)

Elastin is a ubiquitous extracellular matrix protein that is highly organized in heart valves and arteries. Because elastic fiber abnormalities are a central feature of degenerative valve disease, we hypothesized that elastin-insufficient mice would manifest viable heart valve disease.To analyze valve structure and function in elastin-insufficient mice (Eln(+/-)) at neonatal, juvenile, adult, and aged adult stages.At birth, histochemical analysis demonstrated normal extracellular matrix organization in contrast to the aorta. However, at juvenile and adult stages, thin elongated valves with extracellular matrix disorganization, including elastin fragment infiltration of the annulus, were observed. The valve phenotype worsened by the aged adult stage with overgrowth and proteoglycan replacement of the valve annulus. The progressive nature of elastin insufficiency was also shown by aortic mechanical testing that demonstrated incrementally abnormal tensile stiffness from juvenile to adult stages. Eln(+/-) mice demonstrated increased valve interstitial cell proliferation at the neonatal stage and varied valve interstitial cell activation at early and late stages. Gene expression profile analysis identified decreased transforming growth factor-beta-mediated fibrogenesis signaling in Eln(+/-) valve tissue. Juvenile Eln(+/-) mice demonstrated normal valve function, but progressive valve disease (predominantly aortic regurgitation) was identified in 17% of adult and 70% of aged adult Eln(+/-) mice by echocardiography.These results identify the Eln(+/-) mouse as a model of latent aortic valve disease and establish a role for elastin dysregulation in valve pathogenesis.
Increased Vulnerability to Atrial Fibrillation in Transgenic Mice With Selective Atrial Fibrosis Caused by Overexpression of TGF-β1
Authors: Sander Verheule, Toshiaki Sato, Thomas Everett, Steven K. Engle, Dan Otten, Michael Rubart-von der Lohe, Hisako O. Nakajima, Hidehiro Nakajima, Loren J. Field, Jeffrey E. Olgin
Journal: Circulation Research (2004)

Studies on patients and large animal models suggest the importance of atrial fibrosis in the development of atrial fibrillation (AF). To investigate whether increased fibrosis is sufficient to produce a substrate for AF, we have studied cardiac electrophysiology (EP) and inducibility of atrial arrhythmias in MHC-TGFcys 33 ser transgenic mice (Tx), which have increased fibrosis in the atrium but not in the ventricles. In anesthetized mice, wild-type (Wt) and Tx did not show significant differences in surface ECG parameters. With transesophageal atrial pacing, no significant differences were observed in EP parameters, except for a significant decrease in corrected sinus node recovery time in Tx mice. Burst pacing induced AF in 14 of 29 Tx mice, whereas AF was not induced in Wt littermates ( P &lt;0.01). In Langendorff perfused hearts, atrial conduction was studied using a 16-electrode array. Epicardial conduction velocity was significantly decreased in the Tx RA compared with the Wt RA. In the Tx LA, conduction velocity was not significantly different from Wt, but conduction was more heterogeneous. Action potential characteristics recorded with intracellular microelectrodes did not reveal differences between Wt and Tx mice in either atrium. Thus, in this transgenic mouse model, selective atrial fibrosis is sufficient to increase AF inducibility.
Israel's anaesthetists protest at plan to expand role of nurses
Authors: Judy Siegel-Itzkovich
Journal: BMJ (2007)

The Israel Anaesthesiology Society is angry with the country's health minister, Jacob Ben-Yizri, and finance minister, Ronnie Bar-On, for signing a secret agreement aimed at allowing nurses to perform certain anaesthesia procedures.

The society, whose members work in 28 medical centres around the country, declared after learning of the agreement that its members “will not participate in training nurses to give anaesthesia or authorise their activities in operating rooms under our authority.”

Allowing nurses to perform such procedures would require the passage of legislation, but it could not be implemented …
Role of nitric oxide in inflammation and tissue injury during endotoxemia and hemorrhagic shock.
Authors: N S Shah, T R Billiar
Journal: Environmental Health Perspectives (1998)

Since the discovery that nitric oxide (.NO) accounts for the biologic activity of endothelial-derived relaxing factor, a torrent of research over the last decade has focused on its role, protective or detrimental, in myriad pathophysiologic conditions. Recently, increasing attention has focused on .NO as a possible mediator of the severe hypotension and impaired vasoreactivity characteristic of circulatory failure. Given the ubiquitous and complex role of .NO in biologic systems, inhibition of .NO synthesis in experimental and clinical studies of shock has yielded mixed, sometimes contradictory, results. Although overproduction of .NO in the vasculature may result in systemic vasodilation, .NO synthesis has also clearly been shown to have a beneficial role in regulating organ perfusion and mediating cytotoxicity. In this review, the pathophysiologic importance of .NO in septic shock and hemorrhagic shock is discussed, and novel therapeutic strategies for manipulation of .NO formation are examined.
Abstract 2335: Thymoquinone induces the MUC4 mRNA-destabilizing activity of Tristetraprolin
Authors: Hyun Hee Lee, Se-Ra Lee, Sun-Hee Leem
Journal: Cancer Research (2014)

Abstract Tristetraprolin (TTP) is an AU-rich element binding protein that destabilizes ARE-containing mRNAs. We previously reported that TTP suppresses several target genes such as those encoding proto-oncogenes and cytokines in various cancer cells. However, the signaling pathway to induce TTP expression is not well known. In this study, we investigate that Tymoquinone (TQ) which is an active ingredient derived from the medicinal plant Nigella satia induces the TTP mRNA and protein expression in AGS and MDA-MB231. TQ treatment destabilized a luciferase mRNA containing cIAP2, VEGF and MUC4 ARE. Transfected cells were treated with various kinds of MAPK inhibitors, treatment with Sorafenib (RAF inhibitor) and U0126 (MEK-1/2) reduced the TTP induction activity of TQ in a dose-dependent manner. We confirmed that co-treatment with sorafenib/U0126 and TQ also reduced TTP expression. Following TQ downregulated the expression of MUC4 in previous study, we next examined whether the changes of TTP expression affects the expression of MUC4. Overexpression of TTP decreased the stability of MUC4 mRNA and expression level of MUC4. On the contrary, inhibition of TTP using siRNA increased the MUC4 expression. MUC4 mRNA contains two AREs within the 3′UTR and TTP destabilized a luciferase mRNA containing MUC4 mRNA 3′UTR. Analyses of point mutants of MUC4 mRNA 3′UTR demonstrated that ARE2 were mostly responsible for the TTP mediated destabilization of MUC4 mRNA. Our data suggest that TQ enhances the mRNA and protein level of TTP via the modulation of the Raf-MEK MAPK signaling and suppressed cancer cell growth and promotion through destabilizing MUC4 mRNA as TTP novel target gene. Citation Format: Hyun Hee Lee, Se-Ra Lee, Sun-Hee Leem. Thymoquinone induces the MUC4 mRNA-destabilizing activity of Tristetraprolin. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2335. doi:10.1158/1538-7445.AM2014-2335
Delays in accessing primary care
Authors: M. Jiwa, A. Knight
Journal: BMJ (2008)

Need to be understood to prevent adverse health outcomes

Many theories exist about the decision to consult a general medical practitioner.1 2 Key influences include the characteristics of the patient, the medical practice itself, the nature of the disease or symptom, and the time of day. These factors are now of particular interest as we ponder the association between access to doctors and the effect on the prognosis of life limiting illness. Changes in policy on the availability of medical practitioners in the United Kingdom have potential clinical consequences; one of these, delay in seeking timely access to appropriate interventions, is explored in the linked study by Lasserson and colleagues (doi:10.1136/bmj.a1569).3 The study found that the opening hours of general practices seem to influence patients’ healthcare seeking behaviour after a transient ischaemic attack and minor stroke and can increase delay in assessment.

It has been shown repeatedly that people have the greatest confidence in their usual doctor and will procrastinate if faced with the prospect of consulting a different doctor …
Assessment of best single sample for finding chlamydia in women with and without symptoms: a diagnostic test study
Authors: S. A. Schoeman, C. M. W. Stewart, R. A. Booth, S. D. Smith, M. H. Wilcox, J. D. Wilson
Journal: BMJ (2012)

<b>Objective</b> To compare vulvovaginal swabs with endocervical swabs as optimal diagnostic sample for detection of <i>Chlamydia trachomatis</i> infection. <b>Design</b> A diagnostic test study. <b>Setting</b> An urban sexual health centre. <b>Participants</b> 3973 women aged ≥16 years requesting testing for sexually transmitted infections. <b>Interventions </b>Participants took a vulvovaginal swab before routine examination, and clinicians took an endocervical swab during examination. <b>Main outcome measure</b> Diagnosis of chlamydia infection with samples analysed using the Aptima Combo-2 assay; positive results confirmed with the Aptima CT assay. <b>Results</b> Of the 3973 participants, 410 (10.3%) were infected with <i>C trachomatis</i>. Infected women were significantly younger (22 <i>v</i> 25 years, P&lt;0.0001) and more likely to have symptoms suggestive of a bacterial sexually transmitted infection (53% <i>v</i> 41%, odds ratio 1.63 (95% CI 1.30 to 2.04)), be a contact of someone with a sexually transmitted infection (25% <i>v</i> 5%, odds ratio 6.18 (4.61 to 8.30)), clinically diagnosed with cervicitis (17% <i>v</i> 4%, odds ratio 4.92 (3.50 to 6.91)), and have pelvic inflammatory disease (9% <i>v</i> 3%, odds ratio 2.85 (1.87 to 4.33)). When women co-infected with gonorrhoea were included in the analysis, there was an association with mixed ethnicity (10% <i>v</i> 7%, odds ratio 1.53 (1.07 to 2.17)); but when those with gonorrhoea were removed, women of white ethnicity were significantly more likely to have chlamydia (85% <i>v</i> 80%, odds ratio 1.40 (1.03 to 1.91)). On analysis of complete paired results, vulvovaginal swabs were significantly more sensitive than endocervical swabs (97% (95% CI 95% to 98%) <i>v</i> 88% (85% to 91%), P&lt;0.00001); corresponding specificities were 99.9% and 100%. In women with symptoms suggestive of a bacterial sexually transmitted infection, vulvovaginal swabs were significantly more sensitive than endocervical swabs (97% (93% to 98%) <i>v</i> 88% (83% to 92%), P=0.0008), as they were in women without symptoms (97% (94% to 99%) <i>v</i> 89% (84% to 93%), P=0.002). <b>Conclusions</b> Vulvovaginal swabs are significantly better than endocervical swabs at detecting chlamydia in women with and without symptoms suggestive of sexually transmitted infections. In those with symptoms, using endocervical samples rather than vulvovaginal swabs would have missed 9% of infections, or 1 in every 11 cases of chlamydia. <b>Trial registration</b>ISRCTN42867448.
Comparison of the efficacy of a demand oxygen delivery system with continuous low flow oxygen in subjects with stable COPD and severe oxygen desaturation on walking.
Authors: C. M. Roberts, J. Bell, J. A. Wedzicha
Journal: Thorax (1996)

BACKGROUND: Provision of ambulatory oxygen using an intermittent pulsed flow regulated by a demand oxygen delivery system (DODS) greatly increases the limited supply time of standard portable gaseous cylinders. The efficacy of such a system has not previously been studied during submaximal exercise in subjects with severe chronic obstructive pulmonary disease (COPD) in whom desaturation is likely to be great and where usage is often most appropriate. METHODS: Fifteen subjects with severe COPD and oxygen desaturation underwent six minute walk tests performed in random order to compare the efficacy of a demand oxygen delivery system (DODS) with continuous flow oxygen. Walk distance, breathlessness, oxygen saturation, resting time, and recovery time (objective and subjective) were recorded and compared for each walk. RESULTS: Breathing continuous oxygen compared with baseline air breathing improved mean walk distance (295 m versus 271 m) and recovery time (47 seconds versus 112 seconds), whilst the lowest recorded saturation (81% versus 74%) and time desaturated below 90% (201 seconds versus 299 seconds) were reduced. When the DODS was compared with air breathing only the walk distance changed (283 m versus 271 m). A comparison of the DODS with continuous oxygen breathing showed the DODS to be less effective at oxygenating subjects with inferior lowest saturation (78% versus 81%), time spent below 90% (284 seconds versus 201 seconds), time to objective recovery (83 seconds versus 47 seconds), and walk distance (283 m versus 295 m). CONCLUSIONS: Neither of the delivery systems was able to prevent desaturation in these subjects. The use of continuous flow oxygen, however, was accompanied by improvements in oxygenation, walk distance, and recovery time compared with air breathing. The DODS produced only a small increase in walk distance without elevation of oxygen saturation, but was inferior to continuous flow oxygen in most of the measured variables when compared directly.
BET bromodomain inhibition suppresses graft-versus-host disease after allogeneic bone marrow transplantation in mice
Authors: Yaping Sun, Ying Wang, Tomomi Toubai, Katherine Oravecz-Wilson, Chen Liu, Nathan Mathewson, Julia Wu, Corinne Rossi, Emily Cummings, Depei Wu, Shaomeng Wang, Pavan Reddy
Journal: Blood (2015)