tbuckley/cabot-search huggingface dataset:
URINE 8-HYDROXY-2’-DEOXYGUANOSINE IS A MARKER FOR PREDICTING MORTALITY AND MORBIDITY AS WELL AS EVALUATING THE EFFECTIVENESS OF BETA-BLOCKER THERAPY IN PATIENTS WITH CHRONIC HEART FAILURE
Authors: Tekehisa Susa, Shigeki Kobayashi, Takeo Tanaka, Shinichi Okuda, Masahiro Doi, Yasuaki Wada, Jutaro Yamada, Takeshi Ueyama, Shuji Kawamura, Masafumi Yano, Masunori Matsuzaki
Journal: Journal of the American College of Cardiology (2010)
Kinins are generated in vivo following nasal airway challenge of allergic individuals with allergen.
Authors: D Proud, A Togias, R M Naclerio, S A Crush, P S Norman, L M Lichtenstein
Journal: Journal of Clinical Investigation (1983)

Using a recently developed model of nasal challenge, we have obtained data that clearly demonstrate, for the first time, kinin generation during a local allergic reaction in vivo. Allergic individuals (n = 8) and matched nonallergic controls (n = 8) were challenged intranasally with the appropriate antigen and nasal washes were taken before and after challenge. Washes were assayed for kinin, histamine, and [3H]-N-alpha-tosyl-L-arginine methyl ester (TAME)-esterase activity. Increased kinin generation was found by radioimmunoassay (RIA) in the nasal washes of all the allergics (5,560 +/- 1,670 pg/ml) but in none of the controls (38 +/- 16 pg/ml). The presence of kinin was highly correlated with that of histamine and TAME-esterase activity and with the onset of clinical symptoms (P less than 0.001). Serial dilutions of nasal washes produced RIA displacement curves that paralleled the standard curve, and recovery of standard kinins that were added to nasal washes was 100 +/- 4% (n = 14). Kinin recovery was identical in both allergics and controls and did not vary significantly with antigen challenge. The immunoreactive kinin in nasal washes was stable to boiling and not precipitated by ethanol, but completely destroyed by carboxypeptidase B. It was evenly distributed between the sol and gel phases of nasal washes. High performance liquid chromatography analysis of the immunoreactive kinin in nasal washes showed it to be a mixture of lysylbradykinin and bradykinin. We conclude that kinins are produced during local allergic reactions in the nose and may contribute to the symptomatology of the allergic response.
Rationing and the Americans With Disabilities Act
Authors: David Orentlicher
Journal: JAMA (1994)

LAST year, when the Bush administration rejected Oregon's plan to ration medical care, it surprised observers with its reliance on the Americans With Disabilities Act (ADA) as the basis for the rejection.<sup>1</sup>When limitations in health care coverage have been challenged under other laws protecting the disabled, courts have not been sympathetic. In addition, in concluding that quality of life and functional status should not be taken into account at all in making rationing decisions,<sup>2</sup>the Bush administration called into question any serious attempts to ration health care (<i>Am Med News</i>. November 9, 1992:1). Nevertheless, there were problems with the Oregon plan. For example, there was unfair discrimination in Oregon's decision to deny liver transplants to rehabilitated patients with alcoholic liver disease but not to patients with other causes of liver failure.<sup>3</sup>Oregon modified its plan in response to the rejection and in response to further objections
Human hepatic stem cell and maturational liver lineage biology
Authors: Rachael Turner, Oswaldo Lozoya, Yunfang Wang, Vincenzo Cardinale, Eugenio Gaudio, Gianfranco Alpini, Gemma Mendel, Eliane Wauthier, Claire Barbier, Domenico Alvaro, Lola M. Reid
Journal: Hepatology (2011)

Abstract Livers are comprised of maturational lineages of cells beginning extrahepatically in the hepato-pancreatic common duct near the duodenum and intrahepatically in zone 1 by the portal triads. The extrahepatic stem cell niches are the peribiliary glands deep within the walls of the bile ducts; those intrahepatically are the canals of Hering in postnatal livers and that derive from ductal plates in fetal livers. Intrahepatically, there are at least eight maturational lineage stages from the stem cells in zone 1 (periportal), through the midacinar region (zone 2), to the most mature cells and apoptotic cells found pericentrally in zone 3. Those found in the biliary tree are still being defined. Parenchymal cells are closely associated with lineages of mesenchymal cells, and their maturation is coordinated. Each lineage stage consists of parenchymal and mesenchymal cell partners distinguishable by their morphology, ploidy, antigens, biochemical traits, gene expression, and ability to divide. They are governed by changes in chromatin (e.g., methylation), gradients of paracrine signals (soluble factors and insoluble extracellular matrix components), mechanical forces, and feedback loop signals derived from late lineage cells. Feedback loop signals, secreted by late lineage stage cells into bile, flow back to the periportal area and regulate the stem cells and other early lineage stage cells in mechanisms dictating the size of the liver mass. Recognition of maturational lineage biology and its regulation by these multiple mechanisms offers new understandings of liver biology, pathologies, and strategies for regenerative medicine and treatment of liver cancers. (Hepatology 2011;)
Suxamethonium and Post-operative Muscle Pains
Authors: F. Prince-White
Journal: BMJ (1957)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following Suxamethonium and Post-operative Muscle Pains Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Abstract 2210: Quantification of the relative amount of mouse and human DNA in cancer xenografts using species-specific gene length variations
Authors: Ming-Tseh Lin, Li-Hui Tseng, Hirohiko Kamiyama, Mihoko Kamiyama, Phillip Lim, Sarah Wheelan, James Eshleman
Journal: Cancer Research (2010)

Abstract Human cancer cell lines and xenografts are valuable samples for whole-genome sequencing of human cancer. Tumors can be maintained by serial xenografting in athymic (nude) or severe combined immunodeficient (SCID) mice. Critical to next generation based sequencing is the relative proportions of human and mouse DNA. In the current study, we developed a molecular assay to quantify the relative contributions of human and mouse in mixed DNA samples. The assay was designed based on deletion/insertion variation between human and mouse genomes. The percentage of mouse DNA was calculated according to the relative peak heights of PCR products analyzed by capillary electrophoresis. Three markers from chromosomes 9 and 10 accurately predicted the mouse genome ratio and were combined into a multiplex PCR reaction. We used the assay to quantify the relative DNA amounts of 93 mouse xenografts used for a recently reported integrated genomic analysis of human pancreatic cancer. Of the 93 xenografts, the mean % of contaminating mouse DNA was 47%, ranging from 17% to 73%, with 43% of samples having greater than 50% mouse DNA. We then comprehensively compared the human and mouse genomes to identify 370 additional candidate gene loci demonstrating human-mouse length variation. With increasing use of whole genome sequencing of human cancers, this assay should be useful to monitor strategies to enrich human cancer cells from mixed human-mouse cell xenografts. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2210.
A subpopulation of mouse cytotoxic T lymphocytes recognizes allogeneic H-2 class I antigens in the context of other H-2 class I molecules.
Authors: F Kievits, P Ivanyi
Journal: The Journal of Experimental Medicine (1991)

Recently, independent lines of evidence strongly suggested that peptides derived from one foreign major histocompatibility complex (MHC) molecule bound to another MHC molecule can give rise to multiple composite MHC complexes that are able to stimulate allo-(xeno)-reactive T cells. In this study, we describe that in vivo immunization of mice with cells mismatched with the recipient for a single class I antigen results in the induction of CD8+ cytotoxic T lymphocytes (CTL) specific for allogeneic class I locus products (Dd, Kd, Dq) in the context of other class I molecules (Ks, Kd, Kk) present on stimulator cells. Evidently, the target antigen for these class I-restricted alloreactive CTL is not the native class I molecule but peptides derived from endogenous processing of allogeneic class I products presented by class I molecules. Using a combination of limiting dilution and split-well analyses, we estimated for Kk-restricted Dq-specific alloreactive CTL a precursor frequency (CTLpf) that was approximately 10 times lower than the CTLpf for "classical" nonrestricted Dq-specific alloreactive CTL. These data suggest that H-2 class I peptides presented by intact H-2 class I molecules are allostimulatory, supporting the concept that the capacity for presentation of MHC peptides by MHC molecules constitutes a part of the allogeneic immune response.
Phosphaturic effect of dopamine in dogs. Possible role of intrarenally produced dopamine in phosphate regulation.
Authors: J L Cuche, G R Marchand, R F Greger, R C Lang, F G Knox
Journal: Journal of Clinical Investigation (1976)

A possible role for dopamine in phosphate handling by the dog kidney was investigated by intrarenal artery infusions of dopamine. Dopamine increased fractional phosphate excretion both in the presence and absence of control of parathyroid hormone and calcitonin. In addition, dopamine increased both renal blood flow and sodium excretion, however, the phosphaturia was independent of these changes; since 30 min after completion of dopamine infusion, renal blood flow and sodium excretion returned to control levels and phosphate excretion remained elevated. For comparison, the vasodilator isoproterenol increased renal blood flow and sodium excretion without a significant change in fractional phosphate excretion. Thus, the phosphaturic effect of dopamine is probably independent of its vasodilator effect. The phosphaturic effect of dopamine could not be accounted for by subsequent conversion to norepinephrine, since norepinephrine was antiphosphaturic in the dog. The effect of endogenous dopamine on renal phosphate excretion was investigated by intrarenal infusion of the precursor dopa. Dopa was phosphaturic both in the presence and absence of parathyroid hormone and calcitonin. In dogs pretreated with carbidopa, which blocks conversion of dopa to dopamine, dopa was no longer phosphaturic, although the kidney remained responsive to dopamine. It is postulated that dopamine may play a role in the intrarenal regulation of phosphate excretion.
Hypertriglyceridaemia with acitretin use: a proposal for its management in the context of overall cardiovascular risk
Authors: B.C.Y. Chan, N. Reid, K. Armour, R.S. Scott, P.M. George, P.D.L. Maurice
Journal: British Journal of Dermatology (2014)

Journal Article Hypertriglyceridaemia with acitretin use: a proposal for its management in the context of overall cardiovascular risk Get access B.C.Y. Chan, B.C.Y. Chan Department of Dermatology Christchurch Hospital Christchurch New Zealand Search for other works by this author on: Oxford Academic Google Scholar N. Reid, N. Reid Department of Cardiovascular Prevention and Lipids Disorders Service Christchurch Hospital Christchurch New Zealand Search for other works by this author on: Oxford Academic Google Scholar K. Armour, K. Armour Department of Dermatology Christchurch Hospital Christchurch New Zealand Search for other works by this author on: Oxford Academic Google Scholar R.S. Scott, R.S. Scott Department of Cardiovascular Prevention and Lipids Disorders Service Christchurch Hospital Christchurch New Zealand Search for other works by this author on: Oxford Academic Google Scholar P.M. George, P.M. George Department of Cardiovascular Prevention and Lipids Disorders Service Christchurch Hospital Christchurch New Zealand Search for other works by this author on: Oxford Academic Google Scholar P.D.L. Maurice P.D.L. Maurice Department of Dermatology Christchurch Hospital Christchurch New Zealand Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 171, Issue 3, 1 September 2014, Pages 665–667, https://doi.org/10.1111/bjd.13027 Published: 01 September 2014
Enhancement of Cytotoxic T-lymphocyte Response in Aged Mice by a Novel Treatment with Recombinant AdIL-12 and Wild-type Adenovirus in Rapid Succession
Authors: Jian Chen, John Wang, Jun Li, Qi Wu, Fei Chu Lim, PingAr Yang, Hui-Chen Hsu, David T Curiel, John D Mountz
Journal: Molecular Therapy (2008)

A decrease in the expression of Th1 cytokines has been associated with age-related decrease in cytotoxic T-lymphocyte (CTL) function. We utilized an E1-deleted adenovirus (Ad) vector to deliver the murine interleukin-12 (IL-12) gene in order to enhance the antivirus CTL response. Wild-type (WT) Ad was administered 3 days after AdIL-12 treatment, when IL-12 production was at its peak and the anti-Ad antibody response had not yet begun to develop. Before receiving AdIL-12 treatment, aged (18 month old) mice exhibited a 58% decrease in the number of virus-specific CTLs, and a 30% decrease in in vivo CTL activity as compared to young (2 month old) mice. After AdIL-12 treatment, aged mice displayed a greater increase in IL-12 expression and endogenous production of interferon-gamma than observed in young mice. When infected with WT Ad, these AdIL-12-treated aged mice exhibited an increased in vivo CTL response and an in vitro proliferative response that was similar to those in young mice. The frequencies of occurrence of D(b)-E1Bp(+)CD8(+) T cells in the spleen, liver, and lung in aged mice were higher than the corresponding values in young mice. These results indicate that IL-12 treatment significantly promotes the virus-specific CTL response in aged mice and, more importantly, specifically targets the virally infected organs, such as the liver and lung, promoting enhanced CTL activity against the virus.
ethical pitfalls can be hard to avoid
Authors: Duncan Forrest, James Barrett
Journal: BMJ (2004)

Many clinicians will have memories of slightly disturbing encounters—perhaps while in training—when they felt that a patient was suffering unnecessarily thoughtless, humiliating, or frankly brutal treatment. It is difficult for junior doctors to make a complaint. Perhaps it becomes slightly easier with growing experience and seniority, but it is never straightforward and many encounters go by without protest. Although ethical norms have improved in recent years, there are still many working environments where doctors daily witness doubtful practices and have to decide whether to confront or ignore them.1 When should you blow the whistle?

Doctors whose work involves divided loyalties, such as police surgeons, prison doctors, or medical officers in the armed forces, are most likely to encounter these ethical problems. Of course, in countries where torture is practised, doctors in such jobs face more serious choices and risks than doctors in the NHS. If they do not cooperate with their employers, they can risk dismissal or even physical danger to themselves or their …
Cell preselection is key to gene therapy success
Authors: A. Berger
Journal: BMJ (2000)

New cell selection strategies look likely to improve the success of gene therapy for blood disorders. Using a green fluorescent labelling technique, scientists at the Massachusetts Institute of Technology in Cambridge have discovered a trick that greatly improves the proportion of stem cells that successfully integrate the desired gene.

Dr Philippe Leboulche and his team seem to have got around the common problem of “silencing,” whereby a new gene is successfully introduced into the genome of a stem cell but fails to be expressed once cell differentiation occurs.

Although their work …
National Lung Cancer Screening Trial American College of Radiology Imaging Network Specimen Biorepository Originating from the Contemporary Screening for the Detection of Lung Cancer Trial (NLST, ACRIN 6654): Design, Intent, and Availability of Specimens for Validation of Lung Cancer Biomarkers
Authors: Edward F. Patz, Neil E. Caporaso, Steven M. Dubinett, Pierre P. Massion, Fred R. Hirsch, John D. Minna, Constantine Gatsonis, Fenghai Duan, Amanda Adams, Charles Apgar, Rosa M. Medina, Denise R. Aberle
Journal: Journal of Thoracic Oncology (2010)
Induction of endogenous interferon-α and reduction of viremia during hemodialysis in uremic patients with chronic hepatitis C
Authors: M. Sampietro, G. Como, A. Catania, S. Badalamenti, G. Lunghi, A. Orlandi, N. Corbetta, S. Finazzi, A. Carlin, G. Graziani, C. Ponticelli, G. Fiorelli
Journal: Journal of Hepatology (1998)
Inequality in income and mortality in the United States: analysis of mortality and potential pathways
Authors: G. A Kaplan, E. R Pamuk, J. W Lynch, R. D Cohen, J. L Balfour
Journal: BMJ (1996)

To examine the relation between health outcomes and the equality with which income is distributed in the United States.The degree of income inequality, defined as the percentage of total household income received by the less well off 50% of households, and changes in income inequality were calculated for the 50 states in 1980 and 1990. These measures were then examined in relation to all cause mortality adjusted for age for each state, age specific deaths, changes in mortalities, and other health outcomes and potential pathways for 1980, 1990, and 1989-91.Age adjusted mortality from all causes.There was a significant correlation (r = -0.62 [corrected], P < 0.001) between the percentage of total household income received by the less well off 50% in each state and all cause mortality, unaffected by adjustment for state median incomes. Income inequality was also significantly associated with age specific mortalities and rates of low birth weight, homicide, violent crime, work disability, expenditures on medical care and police protection, smoking, and sedentary activity. Rates of unemployment, imprisonment, recipients of income assistance and food stamps, lack of medical insurance, and educational outcomes were also worse as income inequality increased. Income inequality was also associated with mortality trends, and there was a suggestion of an impact of inequality trends on mortality trends.Variations between states in the inequality of the distribution of income are significantly associated with variations between states in a large number of health outcomes and social indicators and with mortality trends. These differences parallel relative investments in human and social capital. Economic policies that influence income and wealth inequality may have an important impact on the health of countries.
Randomised controlled trial of home based care of patients with chronic obstructive pulmonary disease
Authors: O. Hermiz
Journal: BMJ (2002)

To evaluate usefulness of limited community based care for patients with chronic obstructive pulmonary disease after discharge from hospital.Randomised controlled trial.Liverpool Health Service and Macarthur Health Service in outer metropolitan Sydney between September 1999 and July 2000.177 patients randomised into an intervention group (84 patients) and a control group (93 patients) which received current usual care.Home visits by community nurse at one and four weeks after discharge and preventive general practitioner care.Frequency of patients' presentation and admission to hospital; changes in patients' disease-specific quality of life, measured with St George's respiratory questionnaire, over three months after discharge; patients' knowledge of illness, self management, and satisfaction with care at discharge and three months later; frequency of general practitioner and nurse visits and their satisfaction with care.Intervention and control groups showed no differences in presentation or admission to hospital or in overall functional status. However, the intervention group improved their activity scores and the control group worsened their symptom scores. While intervention group patients received more visits from community nurses and were more satisfied with their care, involvement of general practitioners was much less (with only 31% (22) remembering receiving a care plan). Patients in the intervention group had higher knowledge scores and were more satisfied. There were no differences in general practitioner visits or management.This brief intervention after acute care improved patients' knowledge and some aspects of quality of life. However, it failed to prevent presentation and readmission to hospital.
Deep Vein Thrombophlebitis after Acupuncture
Authors: Bradford M. Blanchard
Journal: Annals of Internal Medicine (1991)

Letters1 November 1991Deep Vein Thrombophlebitis after AcupunctureBradford M. Blanchard, MDBradford M. Blanchard, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-115-9-748_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editors: Deep vein thrombophlebitis ("crural phlebitis") associated with acupuncture has been previously reported (1). I report another such occurrence.A 64-year-old healthy, active woman presented with right upper calf pain. She denied previous leg disease, varicosities, or trauma, and she did not smoke or take hormones. The involved upper calf was tender to lateral compression, and the test for Homan sign was positive. No bruises or marks were seen, and no edema was present. A venogram showed thromboses of the deep perforating muscular veins of the upper posterolateral calf. When this finding was reported to the patient, she...Reference1. Ruchkin J. Auriculo-electropuncture in rheumatoid arthritis (a double-blind study). Ter Arkh. 1987;59:26-30. MedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Bradford M. Blanchard, MD PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byInadvertent vascular injury of the aorta or vena cava caused by acupunctureAcupuncture in physiotherapySafety and Side Effects of Acupuncture and Moxibustion as a Therapy for CancerA Cumulative Review of the Range and Incidence of Significant Adverse Events Associated with AcupunctureAcupuncture in the management of myofascial pain and headacheRare but Serious Complications of Acupuncture: Traumatic LesionsBakers Cyst Burst after AcupunctureComplications of Acupuncture 1 November 1991Volume 115, Issue 9 Page: 748-748 Keywords Acupuncture Edema Hormones Veins ePublished: 1 December 2008 Issue Published: 1 November 1991 PDF downloadLoading ...
The role of organ level conditioning on the promotion of engineered heart valve tissue development in-vitro using mesenchymal stem cells
Authors: Sharan Ramaswamy, Danielle Gottlieb, George C. Engelmayr, Elena Aikawa, David E. Schmidt, Diana M. Gaitan-Leon, Virna L. Sales, John E. Mayer, Michael S. Sacks
Journal: Biomaterials (2009)