tbuckley/cabot-search huggingface dataset:
Surface membrane antigen alteration on blood basophils in patients with Hymenoptera venom allergy under immunotherapy
Authors: Robert Siegmund, Heinz Vogelsang, Andreas Machnik, Dieter Herrmann
Journal: Journal of Allergy and Clinical Immunology (2000)

Background: Venom immunotherapy (VIT) provides widespread protection against systemic anaphylactic reactions after a sting of the respective insect. This effect is attributed to a shift from TH2 to TH1. However, because basophils also produce and release cytokines, such as IL-4 and IL-13, they may be part of the cytokine network. The cytokines may regulate basophilic granulocytes, as suggested by the presence of cytokine receptors IL-2Rα, GM-CSFRα, IL-1RII, IL-3R, IL-4R, IL-5R, and IL-6R on basophils from nonallergic donors. Objective: The purpose of this study was to demonstrate that human basophils from subjects allergic to wasp venom undergoing VIT are regulated by cytokines, as shown by the alteration of the expression of cytokine receptors (and other markers). Methods: The expression of the surface interleukin receptors and activation antigens on basophils from 19 nonallergic subjects and 48 patients with wasp venom allergy was investigated before, immediately after, and 1 week after VIT (20 patients only). Results: Basophilic granulocytes in allergic subjects, compared with those in healthy persons, showed elevated expression of CD32 (FcγRII), CD122 (IL-2Rβ), CD124 (IL-4Rα), CD130 (IL-6 and 11Rβ), CD154 (CD40L), and HLA-DR. Activation of basophils clearly increased during VIT indicated by increased expression of CD32, CD33, CD35 (CR1), CD63, CD116 (GM-CSFRα), CD122, CD124, CD130, and CD154. HLA-DR expression also tended to increase. The expression of IL-5R (CD125) decreased. A significant decrease of the basophilic surface antigens CD11c, CD32, CD35, CD63, CD116, CD122, CD124, CD130, and CD132 (interleukin receptor γ) was detected 1 week after the end of rush VIT. Conclusion: The rise in CD63 during VIT indicates a partial basophil degranulation with release of stored protein mediators, including IL-4. IL-4 may cause a transient upregulation of different surface antigens in an autocrine manner. Thereafter, cytokines released by T cells, which as a result of VIT have changed from a TH2 type to a more TH1 type, downregulate the activation of the basophilic granulocytes. (J Allergy Clin Immunol 2000;106:1190-5.)
Reduced TET2 function leads to T-cell lymphoma with follicular helper T-cell-like features in mice
Authors: H Muto, M Sakata-Yanagimoto, G Nagae, Y Shiozawa, Y Miyake, K Yoshida, T Enami, Y Kamada, T Kato, K Uchida, T Nanmoku, N Obara, K Suzukawa, M Sanada, N Nakamura, H Aburatani, S Ogawa, S Chiba
Journal: Blood Cancer Journal (2014)

TET2 (Ten Eleven Translocation 2) is a dioxygenase that converts methylcytosine (mC) to hydroxymethylcytosine (hmC). TET2 loss-of-function mutations are highly frequent in subtypes of T-cell lymphoma that harbor follicular helper T (Tfh)-cell-like features, such as angioimmunoblastic T-cell lymphoma (30–83%) or peripheral T-cell lymphoma, not otherwise specified (10–49%), as well as myeloid malignancies. Here, we show that middle-aged Tet2 knockdown (Tet2gt/gt) mice exhibit Tfh-like cell overproduction in the spleen compared with control mice. The Tet2 knockdown mice eventually develop T-cell lymphoma with Tfh-like features after a long latency (median 67 weeks). Transcriptome analysis revealed that these lymphoma cells had Tfh-like gene expression patterns when compared with splenic CD4-positive cells of wild-type mice. The lymphoma cells showed lower hmC densities around the transcription start site (TSS) and higher mC densities at the regions of the TSS, gene body and CpG islands. These epigenetic changes, seen in Tet2 insufficiency-triggered lymphoma, possibly contributed to predated outgrowth of Tfh-like cells and subsequent lymphomagenesis. The mouse model described here suggests that TET2 mutations play a major role in the development of T-cell lymphoma with Tfh-like features in humans.
Circulating Osteoprotegerin Levels and Long-Term Prognosis in Patients With Acute Coronary Syndromes
Authors: Torbjørn Omland, Thor Ueland, Anna M. Jansson, Anita Persson, Thomas Karlsson, Camilla Smith, Johan Herlitz, Pål Aukrust, Marianne Hartford, Kenneth Caidahl
Journal: Journal of the American College of Cardiology (2008)
Uncleaned Anesthesia Equipment
Authors: Gale E. Dryden
Journal: JAMA (1975)

PAIN RELIEF and patient jeopardy are often partners when general anesthesia is administered through unclean equipment. A 1968 survey<sup>1</sup>of teaching hospitals disclosed that all parts of the anesthesia equipment were not being cleaned, even though cultures of the patient's exposed air passages were positive in many cases. There are scattered reports in the scientific literature proving the pathway of disease transmission from anesthesia equipment.<sup>2-4</sup>The morbidity and mortality statistics resulting directly from unclean anesthesia equipment have not been studied. However, the concept of aseptic technique in medical practice should be sufficient to induce the anesthesia teams to develop bacterially clean systems for each patient. The effectiveness of clean anesthesia systems for general anesthesia has been reported by Albrecht and Dryden,<sup>5</sup>who showed a decrease from 27% to 6% in the incidence of pulmonary complications when all parts of the anesthesia apparatus were cleaned after every use.
Adding Chloroquine to Conventional Treatment for Glioblastoma Multiforme
Authors: Julio Sotelo, Eduardo Briceño, Miguel Angel López-González
Journal: Annals of Internal Medicine (2006)

Background: Malignant cell clones resistant to chemotherapy and radiotherapy frequently lead to treatment failure in patients with glioblastoma multiforme. Preliminary studies suggest that adding chloroquine to conventional therapy may improve treatment outcomes. Objective: To examine the effect of adding chloroquine to conventional therapy for glioblastoma multiforme. Design: Randomized, double-blind, placebo-controlled trial. Setting: National Institute of Neurology and Neurosurgery of Mexico. Patients: 30 patients with surgically confirmed glioblastoma confined to 1 cerebral hemisphere, with a Karnofsky performance score greater than 70, no comorbid disease, and age younger than 60 years. Interventions: Oral chloroquine at 150 mg/d for 12 months beginning on postoperative day 5 or placebo. All patients received conventional chemotherapy and radiotherapy. Measurements: Primary outcome was survival after surgery; surviving patients were followed up to October 2005. Periodic evaluation using the Karnofsky scale and imaging studies, as well as hematologic tests and ophthalmologic examinations, was done in all patients. Results: Median survival after surgery was 24 months for chloroquine-treated patients and 11 months for controls. At the end of the observation period, 6 patients treated with chloroquine had survived 59, 45, 30, 27, 27, and 20 months, respectively; 3 patients from the control group had survived 32, 25, and 22 months, respectively. Although not statistically significantly different, the rate of death with time was approximately half as large in patients receiving chloroquine as in patients receiving placebo (hazard ratio, 0.52 [95% CI, 0.21 to 1.26]; P = 0.139). Limitations: The observed advantage of chloroquine may be due to chance; differences in pretreatment characteristics and conventional treatment regimens could not be adjusted for because of the small sample size. Conclusions: Chloroquine may improve mid-term survival when given in addition to conventional therapy for glioblastoma multiforme. These results suggest that larger, more definitive studies of chloroquine as adjuvant therapy for glioblastoma are warranted.
Halcinonide in the treatment of corticosteroid responsive dermatoses
Authors: EUGENE LEIBSOHN, FILLMORE K. BAGATELL
Journal: British Journal of Dermatology (2006)

Eighty-eight patients with bilateral lesions of atopic dermatitis, eczematous dermatitis, neurodcrmatitis or psoriasis were treated, without occlusion, with halcinonide and with betamethasone 17-valerate creams (0.1%) and evaluated in a double-blind, paired comparison study. In the forty-three psoriatic patients, halcinonidc was superior in twenty-three, betamethasone 17-valerate was superior in four, both drugs were equally effective in fourteen, and neither drug was effective in two. In the evaluation of all eighty-eight patients, halcinonide was superior in forty-two, betamethasone 17-valerate was superior in eight, both drugs were equally effective in twenty-nine, and neither drug was effective in nine. The overall superiority of halcinonide to betamethasone 17-valerate was highly significant (P <0.001). Halcinonide exhibited its superiority each week of the 3-week study. There were no side effects with either drug. The substitution of a chlorine atom for a hydroxyl group in the 21-position of the triamcinolone acctonide molecule, and the use of a specifically formulated cream vehicle containing propylene glycol and water, have yielded an extremely active topical anti-inflammatory drug.
The critical issue of hepatocellular carcinoma prognostic classification: which is the best tool available?
Authors: Umberto Cillo, Marco Bassanello, Alessandro Vitale, Francesco A Grigoletto, Patrizia Burra, Stefano Fagiuoli, Francesco D'Amico, Francesco Antonio Ciarleglio, Patrizia Boccagni, Alberto Brolese, Giacomo Zanus, Davide Francesco D'Amico
Journal: Journal of Hepatology (2003)
GATA believe it: new essential regulators of pancreas development
Authors: Santiago Rodríguez-Seguí, Ildem Akerman, Jorge Ferrer
Journal: Journal of Clinical Investigation (2012)

Understanding the transcriptional mechanisms that underlie pancreas formation is central to the efforts to develop novel regenerative therapies for type 1 diabetes. Recently, mutations in the transcription factor GATA6 were unexpectedly shown to be the most common cause of human pancreas agenesis. In this issue of the JCI, Carrasco et al. and Xuan et al. investigate the role of Gata6 and its paralogue Gata4 in mouse embryonic pancreas and show that GATA factors are essential regulators of the proliferation, morphogenesis, and differentiation of multipotent pancreatic progenitors.
Unilateral Pulmonary Edema after Renal Transplantation
Authors: Mitchell Friedman, Richard Libert, Edward D. Michaelson
Journal: New England Journal of Medicine (1975)

UNILATERAL pulmonary edema has been described after re-expansion of pneumothorax,1 in congenital heart disease,2 after thoracentesis3 and in failure of the left side of the heart.4 The two cases reported below shortly followed renal transplantation.Case ReportsCase 1. A 28-year-old woman with end-state renal failure received a cadaveric kidney transplant. After operation, she was positioned on her right side. Four days later she had dyspnea. There had been a 3.2-kg weight gain. The blood pressure was 190/120 mm Hg. Examination of the chest showed consolidation in the right lower lobe. Arterial-blood gas analysis revealed hypoxemia and hypocapnia. The serum . . .
Abstract 4954: Hematogenous metastasis of ovarian cancer: Re-thinking mode of spread
Authors: Sunila Pradeep, Seung Wook Kim, Sherry Wu, Nishimuara Masato, Takahito Miyake, Choi Hyun-Jin, Rajesha Rupaimoole, Jinsong Liu, Isahai Fidler, Gabriel Lopez, Anil Sood
Journal: Cancer Research (2014)

Abstract Background: Most patients with ovarian cancer have widely metastatic disease within the abdominal cavity at primary diagnosis; historically, this was thought to occur via intraperitoneal “seeding”. Here, we report unexpected findings related to hematogenous ovarian cancer metastasis with a strong tropism for the omentum. Methods: We established a novel parabiosis model where the skin of female mice was fused surgically from the shoulder to the hip joint. Two weeks later, the “host” parabiont mice were injected with tumor cells into ovary. Once the host mice became moribund, the parabionts were separated, and the host mice were euthanized. Tumor development and patterns of metastasis were analyzed in the host mice. A series of additional in vitro and in vivo experiments were carried out to decipher the underlying mechanisms. Results: The parabiosis models demonstrated that tumor cells injected into the ovary of each “host” mouse metastasized to the corresponding “guest” mouse, predominantly to the omentum. Using high-throughput transcriptome analysis and phospho-proteome arrays, we demonstrated that ErbB3, an EGFR family receptor tyrosine kinase (RTK) was highly expressed in the ovarian cancer cells that hematogenously metastasized to the omentum (SKOV3-OM3) compared to parental cells. The ErbB3-NRG1 axis activated Src/PI3K/AKT signaling pathway, which in turn induced loss of polarity and increased invasion of ovarian cancer cells. Depletion of murine NRG1 using target specific siRNAs almost completely abolished omental metastasis. In contrast, overexpression of ErbB3 resulted in enhanced tumor formation and omental metastatic spread, which confirms the role of ErbB3 in hematogenous omental metastasis. Additional studies with a function-blocking antibody specific to ErbB3 led to substantial reduction in hematogenous metastasis of ovarian cancer cells. Conclusions: Collectively, our results provide the first evidence that ovarian cancer cells are capable of hematogenous metastasis, but with strong tropism for the omentum. These findings not only provide a new understanding of ovarian cancer metastasis, but also identify novel therapeutic targets. Citation Format: Sunila Pradeep, Seung Wook Kim, Sherry Wu, Nishimuara Masato, Takahito Miyake, Choi Hyun-Jin, Rajesha Rupaimoole, Jinsong Liu, Isahai Fidler, Gabriel Lopez, Anil Sood. Hematogenous metastasis of ovarian cancer: Re-thinking mode of spread. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4954. doi:10.1158/1538-7445.AM2014-4954
Taurohyodeoxycholate- and tauroursodeoxycholate-induced hypercholeresis is augmented in bile duct ligated rats
Authors: Leonardo Baiocchi, Gianfranco Alpini, Shannon Glaser, Mario Angelico, Domenico Alvaro, Heather Francis, Marco Marzioni, Jo Lynne Phinizy, Barbara Barbaro, Gene LeSage
Journal: Journal of Hepatology (2003)
Cardiovascular response to dynamic exercise in patients with chronic symptomatic mild-to-moderate and severe aortic regurgitation.
Authors: D T Kawanishi, C R McKay, P A Chandraratna, M Nanna, C L Reid, U Elkayam, M Siegel, S H Rahimtoola
Journal: Circulation (1986)

Fifteen patients with symptomatic mild-to-moderate and severe chronic aortic regurgitation (AR) performed supine bicycle exercise while measurements of rest and exercise hemodynamics and left ventricular function were obtained. A continuous Doppler method was used to determine the change in distribution of total left ventricular stroke volume between forward stroke volume and regurgitant volume (RgV) with exercise. The pulmonary arterial wedge pressure (PAWP) was lower in the mild-to-moderate AR group than in the severe AR group at rest (8 +/- 1.2 vs 19 +/- 3.6 mm Hg, p = 0.01) and during exercise (15 +/- 3.9 vs 30 +/- 4.3 mm Hg, p = .02). In all patients there were increases in heart rate (78 +/- 4 to 96 +/- 5 beats/min, p less than .001), forward stroke volume (41 +/- 2 to 46 +/- 2 ml/m2), and the cardiac index (3.1 +/- 0.2 to 4.4 +/- 0.3 liters/min-m2, p less than .001), despite a fall in total left ventricular stroke volume index from 84 +/- 5 to 76 +/- 5 ml/m2 (p = .03). The systemic vascular resistance (SVR) decreased with exercise from 1277 +/- 72 to 1031 +/- 64 dynes-sec/cm5 (p less than .001), and the RgV and regurgitant fraction (RgF) both decreased with exercise from 43 +/- 5 ml/m2 to 30 +/- 4 ml/m2 (p = .002) and 0.50 +/- 0.03 to 0.37 +/- 0.03 (p less than .001), respectively. Left ventricular ejection fraction increased on exercise from 0.51 +/- 0.03 to 0.55 +/- 0.03 (p = .02) for the group, but it either decreased or failed to increase by at least 0.05 in seven of 13 patients. The change in ejection fraction on exercise was directly related to the change in SVR (r = .80, p less than .001). We conclude that: in patients with mild-to-moderate AR, the PAWP is generally normal at rest and exercise, in most of those with severe AR, the PAWP is elevated at rest and increases significantly with exercise, which is the likely mechanism for dyspnea on exertion in these patients, the cardiac index in both groups is normal at rest and increases on exercise, the increase in cardiac output results from both an increased heart rate and forward stroke volume, the increase in forward stroke volume results from reductions of RgV and RgF, the RgV and RgF are decreased due to a decreased SVR, and the ejection fraction response to exercise is variable and correlates best with changes in SVR with exercise.
T'was the Night at the Hospital
Authors: Holly Geyer, Robyn Emanuel, Lynette Scherber
Journal: Annals of Internal Medicine (2012)

Ad Libitum18 December 2012T'was the Night at the HospitalHolly Geyer, MD, Robyn Emanuel, MD, and Lynette ScherberHolly Geyer, MDFrom Mayo Clinic, Scottsdale, AZ 85259, and Brooklyn Park, MN 55444.Search for more papers by this author, Robyn Emanuel, MDFrom Mayo Clinic, Scottsdale, AZ 85259, and Brooklyn Park, MN 55444.Search for more papers by this author, and Lynette ScherberFrom Mayo Clinic, Scottsdale, AZ 85259, and Brooklyn Park, MN 55444.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-157-12-201212180-00015 Audio Reading - “T'was the Night at the Hospital” Audio. Michael A. LaCombe, MD, Annals Associate Editor, reads "T'was the Night at the Hospital," by H. Geyer, R. Emanuel, and L. Scherber. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail T'was the night before Christmas, cross-covering the House,Not a patient unstable, no disease-ridden louse.The monitors bleeped, ST-segments unchanged,While the older demented slept blissfully deranged.And I in my scrubs, eyes bleary and glazed,Sipped my peppermint cocoa, my pager unfazed.When jolted from stupor, no if's, but's, or maybe's,Thunderous beeping began jarring my stapes!I sprang from my call room, lunged toward the ED,Out through to the street—Oh what trauma awaits me?EMT touting gurneys emerged ... Author, Article, and Disclosure InformationAffiliations: From Mayo Clinic, Scottsdale, AZ 85259, and Brooklyn Park, MN 55444.Corresponding Author: Holly Geyer, MD, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259; e-mail, [email protected]. PreviousarticleNextarticle Advertisement Audio Reading - “T'was the Night at the Hospital” Audio. Michael A. LaCombe, MD, Annals Associate Editor, reads "T'was the Night at the Hospital," by H. Geyer, R. Emanuel, and L. Scherber. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ FiguresReferencesRelatedDetails Metrics 18 December 2012Volume 157, Issue 12Page: 914-915KeywordsKidneysLiverLow density lipoproteinLungsMedicarePenicillinPlateletsProphylaxisSpleenSurgery ePublished: 18 December 2012 Issue Published: 18 December 2012 Copyright & PermissionsCopyright © 2012 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Adrenal Steroids and Disease
Authors: A. R. Colwell
Journal: JAMA (1972)

The current edition of this hefty textbook, published eight years after the first, successfully incorporates basic information from more than one thousand articles into principles of corticosteroid therapy. With accurate diagnosis, disturbed endocrine function can often be altered with proper drugs. Although many of the mechanisms involved are still obscure, fastmoving research offers new techniques for application to a variety of disciplines. Evolution of the steroid formulas, allied to the alcohols, is traced from their estrogen beginning. Individual biochemical properties derive from different arrangements of the H, OH and O<sub>2</sub>molecules on the cyclophenanthrene nucleus. Discovery of the nature of protein binding and subsequent development of bioassay methods are discussed. The author gives values for concentration of cortisone in the body fluids, emphasizing those of clinical importance (eg, synovial fluid contains half the amount in circulating blood). Current concepts of cortisone action include the hormonegene hypothesis. Relative significance of
UK government doubles payments to haemophilia patients infected with HIV from contaminated blood but makes no increase for hepatitis C
Authors: C. Dyer
Journal: BMJ (2009)

UK citizens with haemophilia who were infected with HIV from contaminated blood are to have their annual payments from the UK government doubled, but patients infected with hepatitis C will get nothing extra.

The health minister Dawn Primarolo announced that government funding for two trusts, which make discretionary payments to people infected with HIV from contaminated blood, would be increased to allow payments of £12 800 (€14 700; $20 200) a year each. Current payments are about £6400.

But the Skipton Fund, which dispenses money to people infected with hepatitis C, will get no further cash, although the government pledged to review the …
Direct detection of Hb C (B6 Glu-Lys) byBseRI analysis
Authors: N. Akar, E. Akar, H. Taştan, Ş. Cin
Journal: American Journal of Hematology (1996)

Hemoglobin C (B6 Glu-Lys) can be identified by different techniques. Here we describe a restriction enzyme digestion protocol (BseRI GAGGAG N10) for direct detection of this variant. © 1996 Wiley-Liss, Inc.
Delta agent infection in acute hepatitis and chronic HBsAg carriers with and without liver disease.
Authors: A Craxi, G Raimondo, G Longo, G Giannuoli, R De Pasquale, M Caltagirone, S Patti, G Squadrito, L Pagliaro
Journal: Gut (1984)

Hepatitis B virus (HBV) is a major cause of chronic liver disease in southern Italy. In the same area superinfection with the delta agent is endemic. To assess the prevalence of delta infection in a large population of patients with acute and chronic HBV related liver disease and to look for differential features among delta infected and uninfected subjects sera from 592 consecutive HBsAg positive patients were tested for the delta/anti-delta system by RIA. In no case was delta Ag found in serum. The prevalence of anti-delta was low in acute hepatitis (6.6%) and in asymptomatic carriers (6.4%) but raised in chronic active hepatitis with or without cirrhosis (52.3%). A decrease in frequency of anti-delta was seen in inactive cirrhosis (38.8%) and in hepatocellular carcinoma (11.9%). A younger mean age of delta-infected subjects was observed in each type of chronic liver disease. Our data confirm that delta agent superinfection is definitely associated with severe chronic active liver disease. The difference in age between anti-delta positive and negative patients suggests that delta infection accelerates the natural history of HBV related liver disease.