tbuckley/cabot-search huggingface dataset:
Calcium-dependent platelet aggregation through the interaction between phosphatidylserine and LOX-1
Authors: Kazunori Otsui, Makoto Kakutani, Koji Yuasa, Atushi Nakano, Chang-Ping Hu, Akemi Kakino, Yuko Sato, Yoshiko Fujita, Yosuke Kawase, Kou-ichi Jishage, Hiroshi Suzuki, Jawahar L. Mehta, Mitsuhiro Yokoyama, Ken-ichi Hirata, Nobutaka Inoue, Tatsuya Sawamura
Journal: Cardiovascular Research (2010)

Aims Platelet aggregation at the injured surface of blood vessels plays a crucial role in arterial thrombosis. Platelet aggregation in plasma has been analysed in the presence of calcium chelator to avoid coagulation. Here, we describe a novel mechanism of platelet aggregation that involves calcium-dependent binding between phosphatidylserine (PS) and lectin-like oxidized LDL receptor-1 (LOX-1), and the contribution of platelet LOX-1 to arterial thrombosis.

Methods and results In vitro , LOX-1 gene deletion and anti-LOX-1 antibody suppressed the aggregation of platelets from mice and humans, respectively, in the absence of calcium-chelating citrate, but not in the presence of citrate. LOX-1 blockade and annexin V, in parallel, suppressed platelet aggregation and shifted the population of aggregates from large to small in size. Simultaneous application of anti-LOX-1 antibody and annexin V did not exert additive suppressive effects on aggregation. On activation, platelets exposed LOX-1 and PS and bound to a PS-coated surface in an LOX-1-dependent manner. In vivo , both LOX-1 deletion and anti-LOX-1 antibody significantly suppressed thrombus formation in mice and rats, respectively. Furthermore, platelet transfusion from LOX-1 knockout to wild-type mice still showed the suppressive effects of LOX-1 gene deletion, suggesting that platelet LOX-1 promoted thrombus formation.

Conclusion LOX-1 blockade could be a novel approach to prevent platelet aggregation and thrombosis.
Estrogen Treatment for Urinary Incontinence
Authors: Catherine E. DuBeau
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
DNA replication fidelity in Mycobacterium tuberculosis is mediated by an ancestral prokaryotic proofreader
Authors: Jeremy M Rock, Ulla F Lang, Michael R Chase, Christopher B Ford, Elias R Gerrick, Richa Gawande, Mireia Coscolla, Sebastien Gagneux, Sarah M Fortune, Meindert H Lamers
Journal: Nature Genetics (2015)
Electricity for Salpingitis.
Authors: John Kercher
Journal: JAMA (1894)

Chicago, Ill., May 12, 1894.  To the Editor:  —I always find it interesting reading the report of the proceedings of the American Electro-Therapeutic Association, and in theJournalof April 7 an article appeared read before that body by Dr. W. B. Sprague, of Detroit, on A Contribution to the Electro-Therapeusis of Salpingitis. As this subject is of great interest, and as I have found it especially interesting, you will pardon me if I review his article.  The pathology of tubal troubles has been so recently evolved that the therapeusis is still in the embryonic stage. Many of our leading lights declare that there can be no rational therapeusis—that there can be no remedy but extirpation—as if decapitation were a cure for cephalalgia! Yet many men have a firmer faith in nature's economy, and are seeking to aid her efforts at repair by, at least so far as possible, removing
529. Oncolytic Virotherapy Employing flt-1 Driven mda-7/IL-24 Gene Delivery
Authors: Sergey A. Kaliberov, Lyudmila N. Kaliberova, Amy S. Petersen, Valentina Krendelchtchikova, Victor Krasnykh, Donald J. Buchsbaum
Journal: Molecular Therapy (2005)
M protein and protein F act as important determinants of cell-specific tropism of Streptococcus pyogenes in skin tissue.
Authors: N Okada, A P Pentland, P Falk, M G Caparon
Journal: Journal of Clinical Investigation (1994)

The pathogenic gram-positive bacterium Streptococcus pyogenes (group A streptococcus) causes numerous diseases of cutaneous tissue, each of which is initiated after the interaction of the bacterium with the cells of the epidermis. In this study, we show that different surface proteins of S. pyogenes play important roles in determining the cell-specific tropism of the bacterium in skin. Using streptococcal strains with defined mutations in the genes which encode surface proteins in combination with primary cultures of human skin and an in situ adherence assay which uses histological sections of human skin, we show that the M protein of S. pyogenes mediates the binding of the bacterium to keratinocytes, while a second streptococcal surface protein, protein F, directs the adherence of the organism to Langerhans' cells. Characterization of binding revealed that adherence was inhibited by purified streptococcal proteins and pretreatment of both host cells with the protease trypsin. Adherence was only slightly affected by the state of keratinocyte differentiation in vitro, but was considerably modulated in response to environmental conditions known to regulate expression of M protein and protein F, suggesting that the interaction between these bacterial cell-surface structures/adhesins and keratinocytes and Langerhans' cells may play an important role in streptococcal skin disease.
Storage of Semen
Authors: William H. Perloff
Journal: JAMA (1973)

To the Editor.—  I was interested in the recent report by Smith and Steinberger (223:774, 1973) and in theLetter to the Editorby Jerome A. Silbert, M.D., (224:626, 1973) concerning the storage potential of human semen. Having been involved with the original work that Smith and Steinberger report, I can corroborate the fact that spermatozoa morility was adversely affected as time went on. However, I believe that a more important aspect of this study is urgently required.  At this time we do not have definitive data concerning the possible genetic effect of freezing on human spermatozoa; it is certainly not inconceivable that some type of genetic damage may occur and may not be perceived in the gross examination of a newborn infant but might be revealed if follow-up studies were done, including psychological evaluations of the children as they mature. I wonder if this might not be the time
962 ENHANCEMENT IN LIVER NUCLEAR FACTOR-KB (NF-KB) AND ACTIVATOR PROTEIN 1 (AP-1) DNA BINDING IN OBESE PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE
Authors: J. Poniachik, C. Santibanez, D. Haim, G. Tapia, J. Araya, R. Rodrigo, V. Araya, A. Csendes, P. Burdiles, J. Castillo, J. Diaz, F. Maluenda, O. Korn, L. Gutierrez, J. Rojas, G. Rencoret, J. Ibarra, L. Videla
Journal: Journal of Hepatology (2008)
Tactual Sensitivity in Hypochondriasis
Authors: Marie-Anne Haenen, Anton J.M. Schmidt, Mieke Schoenmakers, Marcel A. van den Hout
Journal: Psychotherapy and Psychosomatics (1997)

Background: In his article on amplification, somatization and somatoform disorders Barsky [Psychosomatics 1992;33:28–34] pointed out the importance of studying the perception and processing of somatic and visceral symptoms. Subsequently, it was demonstrated that hypochondriacal patients are not more accurately aware of cardiac activity than a group of non-hypochondriacal patients. Authors concluded that hypochondriacal somatic complaints do not result from an unusually fine discriminative ability to detect normal physiological sensations that non-hypochondriacal patients are unable to perceive. The aim of the present study was to investigate tactual sensitivity to non-painful stimuli in hypochondriacal patients and healthy subjects. Methods: Twenty-seven outpatients with DSM-III-R hypochondriasis and 27 healthy control subjects were compared. In all subjects the two-point discrimination threshold was measured, as well as subjective sensitivity to harmless bodily sensations as measured by the Somatosensory Amplification Scale. Results: It was found that hypochondriacal patients reported more distress and discomfort with benign bodily sensations. The two-point discrimination threshold of hypochondriacal patients was not significantly lower in patients as compared to controls. Conclusions: Hypochondriacal subjects considered themselves more sensitive to benign bodily sensations without being better able to discriminate between two tactual bodily signals. These findings of the present study correspond quite closely to those reported earlier.
Contact urticaria to cow milk
Authors: E. B. Boso, E. P. Brestel
Journal: Allergy (1987)

A patient with contact urticaria to cow milk is presented contact sensitivity was documented to whole cow milk skim milk commercial condensed milk and milk heated to 80°c for 30 min Contact sensitivity was also documented to casein and lactalbumin Unlike a previous report our patient's sensitivity to heated milk precluded its use in the diet. Thus heating milk does not necessarily reduce allergenicity to its component proteins
Patients' experiences of screening for type 2 diabetes: prospective qualitative study embedded in the ADDITION (Cambridge) randomised controlled trial
Authors: Helen Eborall, Richard Davies, Ann-Louise Kinmonth, Simon Griffin, Julia Lawton
Journal: BMJ (2007)

<b>Objectives</b> To provide insight into factors that contribute to the anxiety reported in a quantitative study of the psychological effect of screening for type 2 diabetes. To explore expectations of and reactions to the screening experience of patients with positive, negative, and intermediate results. <b>Design</b> Prospective qualitative interview study of patients attending a screening programme for type 2 diabetes. <b>Setting</b> Seven general practices in the ADDITION (Cambridge) trial in the east of England. <b>Participants</b> 23 participants (aged 50-69) attending different stages in the screening process. <b>Results</b> Participants9 perceptions changed as they progressed through the screening programme; the stepwise process seemed to help them adjust psychologically. The first screening test was typically considered unimportant and was attended with no thought about its implications. By the final diagnostic test, type 2 diabetes was considered a strong possibility, albeit a "mild" form. After diagnosis, people with screen detected type 2 diabetes tended to downplay its importance and talked confidently about their plans to control it. Participants with intermediate results seemed uncertain about their diagnosis, and those who screened negative were largely unaware of their remaining high risk. <b>Conclusions</b> This study helps in understanding the limited psychological impact of screening for type 2 diabetes quantified previously, in particular by the quantitative substudy of ADDITION (Cambridge). The findings have implications for implementing such a screening programme in terms of timing and content.
Glutathione Peroxidase 2 Inhibits Cyclooxygenase-2–Mediated Migration and Invasion of HT-29 Adenocarcinoma Cells but Supports Their Growth as Tumors in Nude Mice
Authors: Antje Banning, Anna Kipp, Stephanie Schmitmeier, Maria Löwinger, Simone Florian, Susanne Krehl, Sophie Thalmann, René Thierbach, Pablo Steinberg, Regina Brigelius-Flohé
Journal: Cancer Research (2008)

The selenoprotein gastrointestinal glutathione peroxidase 2 (GPx2) is up-regulated in a variety of cancer cells with thus far unknown consequences. Therefore, two clones of a human colon cancer cell line (HT-29) in which GPx2 was stably knocked down by small interfering RNA (siRNA; siGPx2) were used to test whether cancer-relevant processes are affected by GPx2. The capacity to grow anchorage independently in soft agar was significantly reduced in siGPx2 cells when compared with controls (i.e., HT-29 cells stably transfected with a scramble siRNA). The weight of tumors derived from siGPx2 cells injected into nude mice was lower in 9 of 10 animals. In contrast, in a wound-healing assay, wound closure was around 50% in controls and 80% in siGPx2 cells, indicating an enhanced capacity of the knockdown cells to migrate. Similarly, invasion of siGPx2 cells in a Transwell assay was significantly increased. Migration and invasion of siGPx2 cells were inhibited by celecoxib, a cyclooxygenase-2 (COX-2)-specific inhibitor, but not by alpha-tocopherol. Selenium supplementation of cell culture medium did not influence the results obtained with siGPx2 cells, showing that none of the other selenoproteins could replace GPx2 regarding the described effects. The data show that GPx2 inhibits malignant characteristics of tumor cells, such as migration and invasion, obviously by counteracting COX-2 expression but is required for the growth of transformed intestinal cells and may, therefore, facilitate tumor cell growth. The data also shed new light on the use of selenium as a chemopreventive trace element: a beneficial effect may depend on the stage of tumor development.
Head-dropping Test
Authors: R. Wartenberg
Journal: BMJ (1952)

This test serves, first of all, in the early diagnosis of paralysis agitans and post-encephalitic Parkinsonism.The latter term is an awkward one, and has already led to such a stylistic horror as " Rombergism," to which " Babinskiism," it is hoped, will not be added.There- fore the generic term " Parkinson's syndrome" is used here, and the terms " idiopathic," " post-encephalitic," etc., are used to distinguish the varieties.It is easy to diagnose a fully developed case of Park- inson's syndrome of any variety.Often there is an immediate impression of the condition which later proves to be correct, although at the time the impression was gained no adequate proof could be offered.How- ever, it is sometimes difficult to diagnose cases that are just beginning to manifest themselves, or abortive cases, the so-called " formes frustes."The beginning and the course of Parkinson's syndrome of any variety is usually insidious and slow, and this makes the diagnosis particularly difficult.In post-encephalitic Parkinson's syndrome it may be impossible to obtain a clear history of encephalitis, or the encephalitis may have occurred as long as 15 or 20 years previously, rendering some histories unreliable.The long so-called pseudo-neurasthenic stage of postencephalitic Parkinson's syndrome, with its manifold and vague symptoms, particularly contributes to the diagnostic confusion.Many and varied morbid conditions must be considered in the differential diagnosis in such cases : diverse degenerative and non-degenerative diseases of the brain, multiple sclerosis, exophthalmic goitre, senile tremor, familial tremor, arthritis, as well as psychoneurotic, neurasthenic, and hysteric states and various intoxications.How difficult to some clinicians the diagnosis may be is drastically demonstrated by the fact that I have seen patients with unmistakable Parkinson's syndrome who previously had been subjected to encephalography and angiography in the search for a tumour.'LIDE-JAD-DROPPING TEST
Trends in the Use of the Pulmonary Artery Catheter in the United States, 1993-2004
Authors: Renda Soylemez Wiener, H. Gilbert Welch
Journal: JAMA (2007)

Although there is now substantial evidence that pulmonary artery (PA) catheterization does not reduce mortality in critically ill patients, it is unknown whether national utilization has decreased in response.To determine trends in PA catheterization use in the United States.A time trend analysis on national estimates of PA catheterization utilization from 1993-2004 using data from all US states contributing to the Nationwide Inpatient Sample. Hospital admissions for those participants aged 18 years or older were assessed, with primary analysis focused on admissions with a medical diagnosis related group and a secondary analysis focused on surgical admissions. PA catheterization was identified by 5 International Classification of Diseases, Ninth Revision procedure codes describing PA or wedge-pressure monitoring, measurement of mixed venous blood gases, or monitoring of cardiac output by oxygen consumption or other technique.Annual PA catheterization use per 1000 medical admissions.Between 1993 and 2004, PA catheterization use decreased by 65% from 5.66 to 1.99 per 1000 medical admissions (risk ratio [RR], 0.35; 95% confidence interval [CI], 0.29-0.42). Among patients who died during hospitalization, a group whose disease severity may be consistent across time, the relative decline was similar, decreasing from 54.7 to 18.1 per 1000 deaths (RR, 0.33; 95% CI, 0.28-0.38). A significant change in trend occurred following a 1996 study that suggested increased mortality with PA catheterization. The decline in utilization was similar in surgical patients (RR, 0.37; 95% CI, 0.25-0.49). Among common diagnoses associated with PA catheterization, the decline was most prominent for myocardial infarction, which decreased by 81% (RR, 0.19; 95% CI, 0.15-0.23), and least prominent for septicemia, which decreased by 54% (RR, 0.46; 95% CI, 0.38-0.54). Sensitivity analyses suggested findings were not due to artifact of changing procedure coding practice.Use of the PA catheter, previously a hallmark of critical care practice, has decreased in the United States during the last decade, possibly due to growing evidence that this invasive procedure does not reduce mortality.
The History of Psychiatry: An Evaluation of Psychiatric Thought and Practice from Prehistoric Times to the Present
Authors: Leon J. Saul
Journal: JAMA (1966)

Franz Alexander, a multilingual internationalist, was always deeply interested and well-informed on social and political developments. As the first graduate of the Berlin Psychoanalytic Institute, he saw the progress of psychoanalysis from its early stages. And as a leader with broad contacts, he was part of its growth to the present. Besides being abreast of their work, Alexander was personally acquainted—often close friends—with most of the leaders not only in psychoanalysis and psychiatry but in all the related fields. This lends an unusual authenticity to his story of developments since World War I in all aspects of the physical and group methods and investigations, as well as the individual. In Dr. Selesnick he had an invaluable assistant.  The result is a book which very few men could have authored. It is a masterpiece of condensation, using to the full one of Alexander's chief talents, namely, the capacity to penetrate to
Erratum: Ectopic expression of c-myc fails to overcome downregulation of telomerase activity induced by herbimycin A, but ectopic hTERT expression overcomes it
Authors: M Akiyama, O Yamada, S Akita, M Urashima, J Horiguchi-Yamada, T Ohno, H Mizoguchi, Y Eto, H Yamada
Journal: Leukemia (2015)
Nothing to be sad about
Authors: T. Dalrymple
Journal: BMJ (2008)

Within a radius of a few miles of the hospital in which I worked were a number of what were once called dosshouses. (We laugh at the Victorians who supposedly covered up the erotically suggestive legs of pianos, but we routinely disguise realities with euphemisms.)

A certain specific kind of solidarity reigned in the dosshouses. The residents arranged for their social security payments to be paid on different days, so there was always money enough for the 3 l bottles of strong cider that were their main interest in life, the lack of which would cause them acute suffering. But that was the only real solidarity they showed.

Reading Maxim Gorky’s play The Lower …
Olsalazine and GI transit in UC.
Authors: S Meyers
Journal: Gut (1988)

Nevertheless, further bacteriological characterisation, including antimicrobial resistance patterns and fermentative reactions in sugars, permitted a clear distinction between six different Clostridium difficile species.We would recommend, therefore, that case clustering of PMC, suggestive of nosocomial cross infection, is evaluated prospectively using bacteriological typing methods.
Transfusion malaria in a man with Christmas disease.
Authors: A E Vartan
Journal: BMJ (1967)

Incontinentia pigmenti (IP) is a genodermatosis that segregates as an X linked dominant trait with male lethality. The disease has been linked to Xq28 in a number of studies. A few affected males have been documented, most of whom have a 47,XXY karyotype. We report a family with two paternally related half sisters, each affected with IP. The father is healthy, clinically normal, and has a 46,XY normal male karyotype. Linkage analysis of 12 polymorphic markers (two X linked and 10 autosomal) confirms paternity. X inactivation studies with the human androgen receptor (HUMARA) indicate that the paternal X chromosome is inactivated preferentially in each girl, implying that this chromosome carries the IP mutation, and that the father is a gonadal mosaic for the IP mutation.
TCT-653 Assessment of endothelial function in patients randomly treated with a polymer-free sirolimus eluting stent and its bare-metal equivalent: results of the VESTASYNC II trial
Authors: Breno Almeida, Jose Costa, Ricardo Costa, Alexandre Abizaid, Mirela Lima, Marco Perin, Amanda Sousa, J. Eduardo Sousa
Journal: Journal of the American College of Cardiology (2012)