tbuckley/cabot-search huggingface dataset:
Abstract 3846: Vitamin D3 potentiates the antitumor effects of arsenic trioxide in human leukemia (HL-60) cells
Authors: Christian Rogers, Clement Yedjou, Paul Tchounwou
Journal: Cancer Research (2012)

Abstract Arsenic trioxide (ATO) is an innovative form of therapy that has recently been found to benefit acute promyelocytic leukemia (APL) patients. Our laboratory has demonstrated that ATO stimulates oxidative stress therefore causing ATO-induced cytotoxicity in human leukemia (HL-60) cells. Pro-oxidants have been known to play a role in free radical-mediated oxidative stress. Vitamin D3, an active metabolite of vitamin D, inhibits the growth of a number of cancer types such as prostate, breast, colorectal, leukemia, and skin cancers. The specific aim of this research was to determine whether co-exposure to vitamin D3 increases the efficacy of ATO through cytotoxicity, oxidative stress and apoptosis associated with increased ATO toxicity in human leukemia (HL-60) cells. In our current investigation, we performed the MTT assay and trypan blue exclusion test to measure cell viability. We also performed the lipid peroxidation assay to determine the levels of malondialdehyde (MDA) production in HL-60 cells co-exposed to ATO and vitamin D3 in combination. The results of trypan blue exclusion and MTT assay indicated that vitamin D3 exposure potentiates the toxic activity of ATO in HL-60 cells in a dose dependent manner. Co-administration of vitamin D3 and ATO resulted in a significant (P&amp;lt;0.05) increase in MDA level compared to ATO alone. A statistically significant and dose-dependent increase (p &amp;lt;0.05) was recorded in annexin V positive cells (apoptotic cells) with increasing doses of vitamin D3 in ATO-treated cells. This finding was confirmed by the result of DNA laddering assay showing clear evidence of DNA fragmentation in ATO and vitamin D3 treated HL-60 cells. Overall, the present study indicates that vitamin D3 potentiates the antitumor effects of ATO at least part, via cytotoxicity, oxidative stress, nucleosomal fragmentation and phosphatidylserine externalization. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3846. doi:1538-7445.AM2012-3846
Geoffrey Neish Barker
Authors: G. Fox
Journal: BMJ (2003)

Consultant ear, nose, and throat surgeon Cheltenham (b 1909; q St Thomas's Hospital, London, 1932; FRCS, DLO), died from cancer of the oesophagus on 11 September 2002.

After studying laryngology in Vienna and junior posts in Birmingham and Derby, Geoffrey Neish Barker was appointed a consultant at Cheltenham General Hospital in 1938. Apart from a short spell in the army during the second world war, he worked there until he retired in 1973. For much of this time he was singlehanded. He undertook the first successful stapedectomy in Gloucestershire. A skilled furniture maker, in retirement he also enjoyed archery, trout fishing, and acting as a guide to a local abbey. He leaves a wife, Mary, and a daughter.
Re: Efficacy of Influenza Vaccine-Reply
Authors: Peter A. Patriarca
Journal: JAMA (1985)

In Reply.—  We certainly agree with Drs Harper and Gibson that influenza virus infections continue to pose a serious threat to the health and well-being of elderly patients and that aggressive efforts to prevent the disease and its associated complications are clearly warranted. We also agree that a systematic and varied approach is necessary for optimal control, which currently consists of annual vaccination of high-risk patients and medical-care personnel and appropriate use of amantadine hydrochloride for prophylaxis and therapy.1Live attenuated influenza vaccines, rimantadine, and ribavirin may prove to be highly effective additions to this armamentarium, but none of these preparations will be licensed and commercially available for several years. In the meantime, we must continue to promote and use measures that are currently available, with inactivated influenza vaccine representing the most practical and least expensive strategy. While research continues to improve the immunogenicity of these vaccines, to say
Letter: Drug-induced red cell aplasia.
Authors: D MacCulloch, J M Jackson, J Venerys
Journal: BMJ (1974)

SIR,-Your leading article (21 September, p. 701) highlights the problems of Negro patients who may have repeated screening tests for sickle-cell disease.Now that the existence of sickle-cell disease in Britain has been recognized and well publicized I feel that the need for screening should be put in the right per- spective on a par with other potentially dangerous problems associated with anaes- thesia and surgery.It is advisable to perform routine screening for haemoglobinopathies on hospital attendance, but should the absence of a sickle-cell test in fit and nonanaemic adults and older children of possible Negro descent be a contraindication to general anaesthesia?This is certainly not the practice anywhere in Africa.The sutgestion that medical cards be carried by these patients is the obvious solution, but logically medical cards should be advisable for most patients and should include not only haemoglobinopathies but other relevant information, such as blood group, drug allergy, therapy with steroids, anticoagulants, etc. for the same reasons as suggesved in the article.
An Address ON SIR THOMAS BROWNE: Delivered at the Physical Society, Guy's Hospital, October 12th
Authors: W. Osler
Journal: BMJ (1905)

As a boy it was my good fortune to come under the influence of a parish priest of the Gilbert White type, who followed the seasons of Nature no less ardently than those of the Church, and whose excursions into science had brought him into con- tact with physic and physicians.Father Johnson, as his friends loved to call him, founder and Warden of the Trinity College School near Toronto, illustrated that angelical con- junction (to use Cotton Mather'a words) of medicine and divinity more common in the sixteenth and seventeenth cen- turies than in the nineteenth.An earnest student of Sir Thomas Browne, particularly of the Religio Medici, he often read to us extracts in illustration of the beauty of the English language, or he would entertain us with some of the author's quaint conceits, such as the man without a navel (Adam), or that woman was the rib and crooked piece of man.The copy which I hold in my hand (J.T. Fields's edition of 1862) my companion ever since my schooldays, is the most precious book in my library.I mention these circumstances in extenuation of an enthusiasm which has enabled me to make this almost complete collection' of the editions of his works I show you this evening, knowing full well the compassionate feeling with which the bibliomaniae is regarded by his saner colleagues.I.-Tnn MAN.The Little Thomas was happy in his entrance upon the stage, October 1gth, i6o5.Among multiplied acknowledge- ments, he could lift up one hand to Heaven (as he says) that he was born of honest parents, "sthat modesty, humility, patience, and veracity lay in the same egg, and came Into the world " with him.0 his father, a London merchant, but little is known.There is at Devonshire House a family picture which shows him to have been a man of fine presence, looking not unworthy of the future philosopher, a child of 3 or 4 years, seated on his mother's knee.She married a second time, Sir Thomas Dutton, a man of wealth and position, who gave his stepson every advantage of education and travel.We lack accurate information of the early years-of the school days at Winchester, of his life at Broadgate Hall, now Pembroke College, Oxford, and of the influences which induced him to study medicine.Possibly he got his inspira-
TCT-783 Successful MitraClip Therapy in Elderly Patients not Amenable to Surgery: Serial Assessment of Echocardiographic and Clinical Variables out to 2 Years
Authors: Michael Schlueter, Britta Goldmann, Volker Rudolph, Edith Lubos, Daniel Lubs, Hendrik Treede, Hermann Reichenspurner, Stefan Blankenberg, Stephan Baldus
Journal: Journal of the American College of Cardiology (2012)
EXPERIENCES WITH ETHYL CHLORID IN GENERAL ANESTHESIA.
Authors: JAMES P. TUTTLE
Journal: JAMA (1900)

For many years ethyl chlorid has been known as a local anesthetic, and to some few as having properties of a general one through inhalation. Its use has never been very general, however, and information concerning it has been very meager until recently. Almost all physicians have used the drug as a local anesthetic, some more and some less extensively. In this manner, it has never compared with cocain, and can never supersede that drug. I do not propose to go into the literature of kelene anesthesia, but during the discussion of general anesthesia, it might be well to make public some experiments I have been making for the past three months with ethyl chlorid as an adjuvant to ether anesthesia. After reading the report of Army Surgeon Weisner, in November last, I determined to give kelene a trial as a general anesthetic. Accordingly, having obtained a graduated tube, with
Sorting of Vesicular Monoamine Transporter 2 to the Regulated Secretory Pathway Confers the Somatodendritic Exocytosis of Monoamines
Authors: Haiyan Li, Clarissa L. Waites, Roland G. Staal, Yuriy Dobryy, Jeanna Park, David L. Sulzer, Robert H. Edwards
Journal: Neuron (2005)
Sustained‐Release Terbutaline in Nocturnal Asthma
Authors: C. J. J. Westermann, B. M.van Weelden, C. D. Laros
Journal: Allergy (1986)

Fifteen adult patients with nocturnal asthma entered a double blind study. They received single evening dose of one sustained‐release (SR) terbutaline tablet a 7.5 mg or an identical placebo tablet for 1 week, each in a randomised cross‐over fashion in addition to their usual medication. In the 12 evaluated patients there was no change in evening PEF but a significant improvement in morning PEF P &lt; 0.05) and in overnight fall of PEF ( P &lt; 0.01) on active treatment. Symptoms improved by both placebo and active medication. Three patients experienced tremor as a side effect. A single evening dose of SR‐terbutaline can be of help in nocturnal asthma.
12. Self-Complementary AAV2 Vectors Transduce Liver with the Same Efficiency as AAV8: The Critical Role of Second-Strand Synthesis in AAV Biology
Authors: Zhijian Wu, Hongzhe Duan, R. Jude Samulski
Journal: Molecular Therapy (2005)

Top of pageAbstract
Previous studies have shown that conventional single-stranded (ss) AAV-2 vectors only transduce a limited number of liver cells, which restricts the use of AAV-mediated liver gene transfer. Recently, we demonstrated that self-complementary (sc) AAV vector significantly increased liver transduction by overcoming the rate-limiting step of second-strand synthesis. Additional studies have shown that cross-packaging of rAAV-2 genome into specific serotype AAV capsids (e.g. AAV6 and AAV8) also increase liver transduction. Although the exact mechanism is not totally clear, the increased transduction by specific serotype AAVs over AAV2 might be related to differences in virus binding, intracellular trafficking, nuclear entry, uncoating or all of the above. In this study, we demonstrate that scAAV genomes packaged into serotypes other than type 2 are also superior to their single-strand counterparts in liver transduction. In addition, we extend these studies to determine if serotype scAAVs show the same hierarchy in transgene expression as that of ssAAV counterparts. 1|[times]|1011 ss vs sc vector genomes carrying liver-specific expression cassette of human factor IX (hFIX) were packaged into serotype 2, 6 and 8 and injected via portal vein into the livers of C57BL/6 mice. Expression of hFIX was monitored over a time course lasting eight weeks. The results showed that among ss vectors, AAV8 gave the strongest expression, followed by AAV6, while AAV2 gave much lower expression at all time points. For each serotype, higher expression of hFIX protein was achieved by sc vector over its ss counterpart. However, the increase of expression by scAAV6 or scAAV8 was not as significant as that of scAAV2 (3 fold vs 50 fold respectively). Interestingly, both ss and scAAV2 exhibited a unique kinetics of hFIX expression, which was distinctly different from AAV6 and 8. hFIX expression by AAV2 increased slowly during eight weeks post injection, while AAV6 and AAV8 reached their peaks rapidly. As a result, at two weeks post injection, scAAV8 and scAAV6 expressed more efficiently than scAAV2, however, scAAV2 exceeded scAAV6 after 4 weeks and was equal to scAAV8 by 8 weeks. Based on these results, the total number of AAV2 virus that enter hepatocyte nuclei is not less than that of AAV8 or AAV6 even though there is a lag phase for expression from AAV2 virions. The unique expression profile of AAV2 might reflect a step post viral binding to cell surface that is inefficient. Our results clearly demonstrate that scAAV-2 will perform equivalent to type 8 in mouse liver. Unlike AAV serotype 8, which has been reported to have the property of wide spread dissemination to other organs, the well-characterized AAV2 vector carrying sc genomes may still be among the most promising candidate vectors for liver-directed gene therapy.
O39 LONG TERM OUTCOME OF MILD HEPATITIS C RECURRENCE AFTER LIVER TRANSPLANTATION: A LARGE PROSPECTIVE STUDY
Authors: M. Gambato, G. Crespo, M.C. Londoño, Z. Mariño, S. Lens, J.A. Carrión, P. Ruiz, I. Alfaro, J. Bosch, R. Miquel, M. Navasa, X. Forns
Journal: Journal of Hepatology (2014)
Differential Diagnosis of Anemia and Cancer
Authors: C. Leite, B. Hoogstraten
Journal: CA A Cancer Journal for Clinicians (1977)

Although anemia is a very common manifestation of cancer, it is present in a moderate to severe degree (hemoglo bin less than nine gm./l00 ml.) in only 20 percent of patients, and then usually in those with advanced disease.1Only occasionally will the symptoms of ane mia be the presenting complaint of the cancer patient.In general, well-being is influenced by several disease manifesta tions, and relief from one can often greatly benefit the patient.It is impor tant, therefore, to understand the mecha nisms of anemia associated with cancer and to apply appropriate therapeutic measures when possible.Very few comprehensive articles have been written on this topic, and most describe only a single form of anemia.Relatively recent articles have been written by Ley,2 Hyman and Harvey,3 both in the mid-1950s, and by Kremer and Laszlo in 1973.@The following ar tide presents an overview of the dif ferential diagnosis and management of 10 causes of anemia that occur with cancer.This includes: anemia of chronic disease; blood loss anemia; aregenera tive anemia; leukoerythroblastic ane mia; pure red cell aplasia; autoimmune
Appraisal of knowledge and attitude of Akwa Ibomites toward a sustainable environment in Nigeria.
Authors: Rosemary D Ebong
Journal: Environmental Health Perspectives (2002)

Developing countries are constantly and continuously confronted with a myriad of health and environmental problems that threaten the efforts to establish and maintain safe air and water supplies. Nigeria, in general, and Akwa Ibom State, in particular, are not isolated from increasing health and sanitation problems. My goal in this study was to determine the knowledge base of men and women related to sanitation and environmental health in Akwa Ibom State. I found no differences in the attitudes of men and women toward the sustainability of health or a healthy environment in the state. However, more sanitation and environmental education is needed as part of long-term strategy for abating sanitation and environmental health problems in this state.
Total smoking ban is accepted in New Zealand
Authors: Nick Wilson
Journal: BMJ (2005)

Improving surveillance of MRSA bacteraemiaData from north west England support Oxfordshire findings Editor-Wyllie et al conclude that admission cultures accounted for 24% of total hospital methicillin resistant Staphylococcus aureus (MRSA) bacteraemia episodes over seven years to March 2004. 1 Data collected from five district general hospitals and one university hospital in Cheshire and Merseyside and one district general hospital in greater Manchester support this view.This group of hospitals has collected enhanced MRSA bacteraemia surveillance data using published case definitions from the Department of Health since April 2004.Anonymised data are collected on paper forms and analysed using the FORMIC data capture system.In all, 534 cases of S aureus bacteraemia were identified between April 2004 and March 2005, 334 being methicillin sensitive S aureus bacteraemia and 200 MRSA bacteraemia.Sixty six of the MRSA bacteraemia cases occurred within 48 hours of arrival in hospital, 16 being related to a previous hospital admission, 25 in patients known to have been colonised with MRSA, and 13 in patients admitted from nursing or residential homes.Thirty five cases were admitted as medical specialties, 25 of them in patients over 65.Our results support the suggestion by Wyllie et al that anti-MRSA treatment should be considered for patients with possible staphylococcal sepsis who have previously been admitted or known to be colonised with MRSA.In a predominantly district general hospital setting, anti-MRSA treatment should also be considered for possible staphylococcal sepsis in elderly medical admissions and admissions from nursing homes.
Studies in Neurology
Authors: Kenneth R. Magee
Journal: JAMA (1970)

Sir Charles Symonds is a preeminent neurologist whose career has spanned the 40-year period marking the emergence of neurology as a major specialty. For this reason, if no other, a new book compiling his writings must be regarded as a major publishing event. But in addition, this particular book offers unique values: The contents are the papers the author himself selected as his most important contributions; the writing is exceptionally strong and lucid; and the whole provides a capsule-history not only of the specialty but the leaders who shaped the specialty. Neurologists to whom the papers are well known will be glad to have them gathered together in this form; physicians and students in training will find it a valuable source of basic and varied information. Two features are of particular interest—the "autobiographic introduction" and the brief background sketches introducing the separate papers. The author's developmental years were spent with
5Regulation of myogenesis via kinase driven activation of DPF3a, a BAF complex member and its interaction with transcription repressor HEY1
Authors: H Cui, J Schlesinger, V Bansal, I Dunkel, D Meierhofer, S Rickert-Sperling
Journal: Cardiovascular Research (2014)

Purpose: Interactions between chromatin-associated proteins and transcription factors play an important role in chromatin activation and gene regulation. They can be modulated by extracellular kinases, which are frequently activated by hormones and can phosphorylate chromatin-associated proteins to induce gene transcription. DPF3 is a member of the BAF (brahma-associated factor) chromatin remodeling complex and functions as an epigenetic factor to initiate gene transcription during heart and muscle development. Previous studies identified two isoforms of DPF3. DPF3b contains two PHD (plant homeo domain) fingers that recognize acetylated and methylated lysine residues of histone H3 and H4. However, very little is known of DPF3a, which contains a half-PHD finger. Here, we present the molecular mechanism of DPF3a regulation and its signaling cascade in myogenesis. Methods and Results: Using co-immunoprecipitation, we found that HEY proteins (HEY1, HEY2 and HEYL) are associated with BAF complex via the specific interaction with DPF3a. Further experiments showed that the posttranslational modification of DPF3a is essential for the interaction, which is mediated by the half-PHD finger of DPF3a. Using mass spectrometry, we identified three phosphorylation sites within DPF3a and in Tandem Affinity Purification we observed an interaction of DPF3a with all three subunits of the serine/threonine kinase CKII (casein kinase II). In vitro kinase assay showed that DPF3a is phosphorylated by CKII and inhibition of CKII by RNAi significantly reduces the DPF3a phosphorylation in vivo. DPF3a and HEY1 have common genomic targets; however, DPF3a is not capable of binding DNA directly. HEY1 knockdown in C2C12 skeletal muscle cells, combined with ChIP-qPCR demonstrated that the association of DPF3a with its targets is significantly reduced. Thus, HEY1 may represent the linker between DPF3a and its genomic targets. Interestingly, we found that the C-term of DPF3a shows high transcriptional activity in GAL4 transactivation assay and DPF3a significantly reduces HEY1 mediated repression of its targets (e.g. ANF, HEY1 and JAG1) using luciferase reporter assays. Conclusion: Our findings demonstrate an unprecedented mechanism of CKII-depending release of HEY1 from its genomic targets mediated by DPF3a and reveal a link between kinase activity and gene transcription mediated by a member of the BAF complex.
A Telemetering Capsule for Gastrointestinal pH Measurement
Authors: Edward H. Storer
Journal: JAMA (1961)

THE QUANTITATIVE determination of the gastric acidity is important to the clinician because of the relationship between acid-pepsin, peptic ulcer, and gastric carcinoma. The clinical investigator also is interested in such determinations since he needs valid, quantitative data on the physiological responses of the gastrointestinal tract to food, sleep, and mental and physical stress. There is no indisputably physiological method available that yields such data. Gastric acidity is usually studied by inserting a tube into the empty stomach via the nose or mouth. This method has yielded much valuable information<sup>1</sup>but has the following objections: (1) The secretory response of only the empty stomach can be measured since food would clog the tube. (2) Aspiration of the acid gastric juice may cause an artificially high rate of secretion since the inhibitory effect of acid in the stomach is removed. (3) The presence of the tube in the nose or
Lysophosphatidylcholine and Reactive Oxygen Species Mediate the Synergistic Effect of Mildly Oxidized LDL With Serotonin on Vascular Smooth Muscle Cell Proliferation
Authors: Takuya Watanabe, Rajbabu Pakala, Shinji Koba, Takashi Katagiri, Claude R. Benedict
Journal: Circulation (2001)

Background —Mild oxidation of LDL enhances its atherogenic potential and induces a synergistic interaction with serotonin (5HT) on vascular smooth muscle cell (VSMC) proliferation. Because of its complex chemical nature, the mitogenic components of mildly oxidized LDL (moxLDL) remain unclear. Methods and Results —We examined both the effects of lysophosphatidylcholine (LPC) and hydrogen peroxide (H 2 O 2 ), a donor of reactive oxygen species, as major components of moxLDL and their interactions with 5HT on VSMC proliferation. Growth-arrested VSMCs were incubated with different concentrations of moxLDL, LPC, H 2 O 2 , or LPC with H 2 O 2 in the absence or presence of 5HT. DNA synthesis in VSMCs was examined by [ 3 H]thymidine incorporation. MoxLDL, LPC, H 2 O 2 , and 5HT stimulated DNA synthesis in a dose-dependent manner. MoxLDL had a maximal stimulatory effect at a concentration of 5 μg/mL (211%), LPC at 15 μmol/L (156%), H 2 O 2 at 5 μmol/L (179%), and 5HT at 50 μmol/L (205%). Added together, moxLDL (50 ng/mL) and 5HT (50 μmol/L) synergistically increased DNA synthesis (443%). Coincubation of LPC (1 μmol/L) with H 2 O 2 (0.5 μmol/L) and 5HT (5 μmol/L) resulted in a synergistic increase in DNA synthesis (439%), which was nearly equal to that of moxLDL with 5HT (443%). The combined effects of LPC, H 2 O 2 , and 5HT on DNA synthesis were completely reversed by the combined use of an antioxidant, N -acetylcysteine (400 μmol/L) or butylated hydroxytoluene (20 μmol/L), with a 5HT 2 receptor antagonist, LY281067 (10 μg/mL). Conclusions —Our results suggest that both LPC and reactive oxygen species may contribute to the mitogenic effect of moxLDL on VSMCs and its synergistic effect with 5HT.