tbuckley/cabot-search huggingface dataset:
Profile of human T cell response to leishmanial antigens. Analysis by immunoblotting.
Authors: P C Melby, F A Neva, D L Sacks
Journal: Journal of Clinical Investigation (1989)

Control and resolution of leishmanial infection depends primarily on T cell-mediated immune mechanisms. The nature of the leishmanial antigens involved in eliciting T cell immunity is unknown. We have examined the pattern of peripheral blood lymphocyte responses in patients with active, healed, or subclinical leishmanial infection to fractionated leishmanial antigens using a T cell immunoblotting method in which nitrocellulose-bound leishmanial antigen, resolved by one or two dimensional electrophoresis, are incorporated into lymphocyte cultures. The proliferative and IFN-gamma responses of cells from patients with healed mucosal or cutaneous leishmaniasis were remarkably heterogeneous and occurred to as many as 50-70 distinct antigens. In contrast, responses from subjects with active, nonhealing, diffuse cutaneous leishmaniasis were either absent or present to only a small number of antigens. Control and resolution of leishmaniasis, and resistance to reinfection, is therefore associated with a T cell response to a large and diverse pool of parasite antigens. The method of T cell immunoblotting appears to offer a powerful, rapid, and relatively simple approach to the identification of antigens involved in eliciting a T cell response in human leishmaniasis.
Urinary Extravasation Detected by Technetium-99m Bone Scan
Authors: SU-MEI CHEN, TZU-CHEN YEN, SHIN HWA YEH
Journal: Clinical Nuclear Medicine (1994)

A 24-year-old woman with abdominal pain 2 months after resection of ovarian mucinous cystadenocarcinoma underwent bone scintigraphy. A patchy area of MDP accumulation along the left-side of the abdominal cavity was Incidentally noted. Serial Tc-99m DTPA renal scan studies showed enlargement of left kidney with persistent accumulation of radiotracer in the para-renal space, suggesting left hydronephrosis associated with left renoperitoneal fistula. Urinary extravasation was confirmed by intravenous pyelogram, contrast enhanced CT scan, and left nephrostomy with adequate urine drainage.
Antenatal diagnosis of thalassaemia major.
Authors: D V Fairweather, B Modell, V Berdoukas, B P Alter, D G Nathan, D Loukopoulos, W Wood, J B Clegg, D J Weatherall
Journal: BMJ (1978)

Haemoglobin synthesis was studied in fetal blood samples obtained at 17 to 20 weeks' gestation in 22 women at risk of carrying a fetus with homozygous beta-thalassaemia. A presumptive diagnosis of homozygous beta-thalassaemia was made in four cases, and the pregnancy was terminated. An inconclusive answer was obtained in one case, and the patient also chose to have her pregnancy terminated. Two fetuses were lost as a result of the procedure. Of the remaining 15 pregnancies, 13 proceeded to term and two to 36 weeks; in each case a normal infant or one heterozygous for beta-thalassaemia was delivered. Current efforts should be directed towards improving the blood sampling technology so that fetal blood sampling can be used widely in those countries where thalassaemia is a major problem.
Prolonged Suppression of Cough After Inhalation of Lidocaine in a Patient With Sarcoid
Authors: Robert V. Sanders
Journal: JAMA (1984)

CHRONIC persistent cough can present both difficult diagnostic and therapeutic problems. Recent studies have primarily addressed the diagnostic evaluation of chronic cough, and these studies suggest that adequate treatment of the cause is highly effective in suppressing the cough.<sup>1,2</sup>In some patients, however, chronic cough may be caused by pulmonary disease that is unresponsive to therapy. Indeed, chronic cough may be more disabling than any other pulmonary symptom, such as dyspnea, and suppression of the cough then becomes a major therapeutic goal. We describe a patient with sarcoid with disabling cough who had an inadequate response to codeine, but who has had prolonged suppression of his cough after inhalation of nebulized lidocaine. <h3>Report of a Case</h3> A 34-year-old man was diagnosed as having pulmonary sarcoid in 1976 by chest roentgenogram and clinical course, confirmed in 1978 by mediastinal lymph node biopsy specimen showing noncaseating granulomata. He has taken prednisone
Poppers-Associated Retinal Toxicity
Authors: Catherine Vignal-Clermont, Isabelle Audo, José-Alain Sahel, Michel Paques
Journal: New England Journal of Medicine (2010)

from teaching and research institutions in France or abroad, or from public or private research centers.L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Abstract LB-278: Transcriptomic profiling of castrate resistant prostate cancer cells: identification of transcription factors associated with androgen resistance
Authors: Maria Prencipe, Stephen Madden, Amanda O'Neill, Aedin Culhane, Darran O'Connor, Helmut Klocker, William M. Gallagher, William Watson
Journal: Cancer Research (2011)

Abstract Locally advanced and metastatic prostate cancer are treated by hormone ablation therapy. However, despite an initial response, the majority of men relapse within 2–3 years to develop a castrate resistant disease for which there are no effective treatments; therefore defining the mechanisms of resistance represents a significant question. Androgen-independent (AI) sublines were derived from the androgen-dependent (AD) LNCaP cells. Previous studies in our laboratory have shown a complex interplay between changes in pro and anti-apoptotic proteins in the development of this resistance. Manipulating individual genes has only minor alterations in the resistant phenotype so we hypothesise that targeting the central signalling pathways and transcription factors (TFs) would represent a better therapeutic targeting approach. We have undertaken a transciptomic analysis of the LNCaP parental (AD), LNCaP Abl (AI) and Hof (AI) cell lines, using the Affymetrix HG U133 Plus 2.0 GeneChip. Using a combination of correspondence analysis, between group analysis and co-inertia analysis, we have generated a transriptomic profile showing 900 genes associated with androgen resistance. Most importantly, we have predicted a list of TFs including HSF1, CDP, VDR-RXR, SRF and PBX1, which may be responsible for the differential gene expression observed in the AI compared to the AD cells. Taqman validation of 32 genes, including 6 TFs, has confirmed our gene chip results. Further validation of VDR-RXR and SRF activity was performed using luciferase assays. We are currently focusing on SRF, which has recently been associated with prostate cancer development (1). We have confirmed its up-regulation in the AI cell lines by WB. Due to the heterogeneous nature of prostate cancer and the complex changes associated with the development of androgen resistance, the upstream TFs may represent better therapeutic targets to reverse this resistance. Reference 1. Gorlov et al. Prioritizing genes associated with prostate cancer development. BMC Cancer 2010, 10:599 Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-278. doi:10.1158/1538-7445.AM2011-LB-278
THE NATURE OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTIC EXTRACT
Authors: Wilfred H. Manwaring
Journal: The Journal of Experimental Medicine (1912)

1. The bactericidal agent extracted from horse leucocytes is apparently precipitated quantitatively by full saturation with ammonium sulphate. 2. The bactericidal agent is apparently precipitated by absolute alcohol, and is not rendered insoluble by a short contact with alcohol. The agent resembled in this feature certain enzymes which can be isolated and purified by alcoholic precipitation.
Effect of a new phosphodiesterase type III inhibitor as a coronary vasodilator and positive inotropic agent
Authors: A. HARTMANN, M. SAEED, G. SUTSCH, R. J BING
Journal: Cardiovascular Research (1987)

Isolated rabbit coronary arteries, aortic strips, and a working rabbit heart preparation were used to assess the positive inotropic and vasodilator effects of a newly developed positive inotropic compound, CI 914. The substance had a relaxing effect on histamine preconstricted coronary arteries and aortic strips. At concentrations of 10−4 mol·litre−1 the relaxing effect on the coronary arteries was significant only at a histamine concentration of 3 × 10−4 mol·litre−1 (p<0.01) whereas at a concentration of 10−3 mol·litre−1 the relaxing effect was significant at histamine concentrations of from 3 × 10−5 mol·litre−1 to 3 × 10−4 mol·litre−1. Aortic strips were more sensitive to the relaxing effect of CI 914, which was significant with 10−4 mol·litre−1 at histamine concentrations of 3 × 10−6 to 3 × 10−4 (p<0.05 top <0.01). In the isolated working heart preparation topically sprayed histamine significantly increased total and large coronary artery resistances (p<0.001 and p<.01). This effect was significantly reduced (p<0.01 and p<0.02) when CI 914 10−4 mol·litre−1 was present in the perfusion fluid. These findings show that the phosphodiesterase inhibitor CI 914, a positive inotropic agent, induces vasodilatation in isolated coronary arteries and aortic strips of the rabbit and reduces the constriction of coronary arteries induced by histamine in the isolated working rabbit heart preparation.
Pseudogout Attacks in Hospital Patients.
Authors: J. Desmond O'Duffy
Journal: Annals of Internal Medicine (1973)

Meeting Abstracts1 May 1973Pseudogout Attacks in Hospital Patients.J. Desmond O'Duffy, M.D., F.A.C.P.J. Desmond O'Duffy, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-78-5-834_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptIn an effort to elucidate the as yet unknown factors that predispose to pseudogout, patients who had had attacks of pseudogout while hospitalized were reviewed. In 1970 and 1971, 28 such patients were seen (19 men and 9 women) with ages ranging from 53 to 92 years (mean, 70 years); 10 of these patients were seen during their first episode of pseudogout. A total of 31 episodes were observed. Twenty-one of the 28 patients were recovering from an operation.Postoperative attacks occurred at a mean of 6.5 days (median, 3 days). A large number (11) occurred after orthopedic surgery, especially... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Rochester, Minnesota. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1973Volume 78, Issue 5Page: 834-834KeywordsOrthopedic surgery Issue Published: 1 May 1973 PDF downloadLoading ...
Allogeneic Hematopoietic Stem-Cell Transplantation for Patients 50 Years or Older With Myelodysplastic Syndromes or Secondary Acute Myeloid Leukemia
Authors: ZiYi Lim, Ronald Brand, Rodrigo Martino, Anja van Biezen, Jürgen Finke, Andrea Bacigalupo, Dietrich Beelen, Agnes Devergie, Emilio Alessandrino, Roel Willemze, Tapani Ruutu, Marc Boogaerts, Michele Falda, Jean-Pierre Jouet, Dietger Niederwieser, Nicolaus Kroger, Ghulam J. Mufti, Theo M. De Witte
Journal: Journal of Clinical Oncology (2009)

This study was performed to examine the characteristics of transplant activity for patients with myelodysplastic syndromes (MDS) older than 50 years within the European Group for Blood and Marrow Transplantation, and to evaluate the factors predicting outcome within this group of patients.We performed a retrospective multicenter analysis of 1,333 MDS patients age 50 years or older who received transplantation within the EBMT since 1998. The median recipient age was 56 years, with 884 patients (66%) age 50 to 60 years and 449 (34%) patients older than 60 years. There were 811 HLA-matched sibling (61%) and 522 (39%) unrelated donor transplants. Five hundred patients (38%) received standard myeloablative conditioning (SMC), and 833 (62%) received reduced intensity conditioning (RIC).The 4-year estimate for overall survival of the whole cohort was 31%. On multivariate analysis, use of RIC (hazard ratio [HR], 1.44; 95% CI, 1.13 to 1.84; P < .01) and advanced disease stage at transplantation (HR, 1.51; 95% CI, 1.18 to 1.93; P < .01) were associated with an increased relapse rate. In contrast, advanced disease stage at transplantation (HR, 1.43; 95% CI, 1.13 to 1.79; P = .01), use of an unrelated donor (P = .03), and RIC (HR, 0.79; 95% CI, 0.65 to 0.97; P = .03) were independent variables associated with nonrelapse mortality. Advanced disease stage at transplantation (HR, 1.55; 95% CI, 1.32 to 1.83; P < .01) was the major independent variable associated with an inferior 4-year overall survival.Allogeneic hematopoietic stem-cell transplantation remains a potential curative therapeutic option for many older patients with MDS. In this analysis, disease stage at time of transplantation, but not recipient age or the intensity of the conditioning regimens, was the most important factor influencing outcomes.
Dehydration in a Breast-Fed Infant
Authors: Alan R. Rushton
Journal: JAMA (1982)

<h3>To the Editor.—</h3> We were interested in the report by Rowland et al entitled "Malnutrition and Hypernatremic Dehydration in Breast-Fed Infants" (1982;247:1016). A recent experience with a similar case prompted this letter. The patient was a 3,125-g firstborn male infant born at another institution after a full-term uncomplicated pregnancy. The parents were 35 years old, and had attended prenatal classes in which breast-feeding was discussed. The child appeared healthy after birth and nursed well in the hospital. He was discharged on the fourth day of life, weighing 2,756 g. He nursed ten to 15 minutes at each breast approximately every two hours. He seemed alert and content. The parents were unaware of his urine output. He was seen at our institution at age 6 days because the parents had noted a red stain on his diaper. The parents were both healthy and denied use of any medications. The family history
AN EYE LESION FOLLOWING TWO INTRAVENOUS INJECTIONS OF SALVARSAN, BUT RELIEVED BY ITS FURTHER USE
Authors: Harold J. Levis
Journal: JAMA (1913)

<h3>Previous History</h3> —The patient, A. M., a man aged 22, in whom a primary lesion appeared in March, 1912, first consulted a homeopathic practitioner, who dispensed tablets of the protoiodid of mercury. After three weeks' use of his drug, the patient became badly salivated and consequenly stopped mercurial treatment. He was then advised by a layman to treat with a certain Detroit physician, who, he was told, would cure him without the use of mercury. His adviser said that the father of this physician had "cured" him about twenty years before without mercury. The patient wrote to the Detroit physician, who replied that he could furnish any number of testimonials concerning the efficacy of his treatment, which he guaranteed contained no mercury. I found this claim to be untrue. A graduate pharmacist made a partial qualitative analysis and found that the preparation he used did contain mercury. The patient at this
Abstract 5367: DNA repair capacity, gene expression and epigenomic profiles in bladder cancer
Authors: Barbara Pardini, Alessandra Allione, Simonetta Guarrera, Valentina Turinetto, Giovanni Fiorito, Fabio Rosa, Alessia Russo, Federica Modica, Claudia Giachino, Paolo Vineis, Carlotta Sacerdote, Giuseppe Matullo
Journal: Cancer Research (2014)

Abstract In Europe, bladder cancer (BC) is the sixth most commonly diagnosed tumor and the second most common cause of death among patients with genitourinary tract malignancies. The ability to repair DNA damage is strongly associated with the risk of cancer and inter-individual variation in DNA repair capacity (DRC) might account for different susceptibility of developing cancer. DRC represents a complex marker comprising the sum of several factors such as gene variants, gene expression, stability of gene products, and effect of inhibitors/stimulators. Individuals with low DRC will tend to accumulate more damage than those who have a better ability to repair such damage. This variability is modulated by the genetic background to which SNPs/haplotype combinations in DNA repair genes, as well as epigenetic regulation are likely to contribute. Phenotypic assays to evaluate DNA repair activity (in particular NER comet assay, H2AX phosphorylation and micronucleus assay) were performed on cryopreserved lymphocytes from 159 cases (collected before treatment) and 159 controls matched by age and smoking habits, enrolled in Turin Bladder Cancer Study (TBCS). We aimed at studying the relationship between DRC (evaluated by comet assay, micronuclei and H2AX phosphorylation assay) and bladder cancer integrating, gene expression and epigenetic profile data (methylation levels and microRNA expression). In particular, we performed an integrated analysis on the genotype/phenotype correlation in a population of bladder cancer cases and controls provided with detailed description of the follow-up for response to therapy/recurrence/survival. This integrated approach will help in elucidating the role of DRC (and its determinants) as predictive and prognostic marker and to identify combined biomarkers to be measured in blood cells as non-invasive predictive method. Citation Format: Barbara Pardini, Alessandra Allione, Simonetta Guarrera, Valentina Turinetto, Giovanni Fiorito, Fabio Rosa, Alessia Russo, Federica Modica, Claudia Giachino, Paolo Vineis, Carlotta Sacerdote, Giuseppe Matullo. DNA repair capacity, gene expression and epigenomic profiles in bladder cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5367. doi:10.1158/1538-7445.AM2014-5367
Replacing and Additive Horizontal Gene Transfer in Streptococcus
Authors: Sang Chul Choi, Matthew D. Rasmussen, Melissa J. Hubisz, Ilan Gronau, Michael J. Stanhope, Adam Siepel
Journal: Molecular Biology and Evolution (2012)

The prominent role of Horizontal Gene Transfer (HGT) in the evolution of bacteria is now well documented, but few studies have differentiated between evolutionary events that predominantly cause genes in one lineage to be replaced by homologs from another lineage ("replacing HGT") and events that result in the addition of substantial new genomic material ("additive HGT"). Here in, we make use of the distinct phylogenetic signatures of replacing and additive HGTs in a genome-wide study of the important human pathogen Streptococcus pyogenes (SPY) and its close relatives S. dysgalactiae subspecies equisimilis (SDE) and S. dysgalactiae subspecies dysgalactiae (SDD). Using recently developed statistical models and computational methods, we find evidence for abundant gene flow of both kinds within each of the SPY and SDE clades and of reduced levels of exchange between SPY and SDD. In addition, our analysis strongly supports a pronounced asymmetry in SPY–SDE gene flow, favoring the SPY-to-SDE direction. This finding is of particular interest in light of the recent increase in virulence of pathogenic SDE. We find much stronger evidence for SPY–SDE gene flow among replacing than among additive transfers, suggesting a primary influence from homologous recombination between co-occurring SPY and SDE cells in human hosts. Putative virulence genes are correlated with transfer events, but this correlation is found to be driven by additive, not replacing, HGTs. The genes affected by additive HGTs are enriched for functions having to do with transposition, recombination, and DNA integration, consistent with previous findings, whereas replacing HGTs seen to influence a more diverse set of genes. Additive transfers are also found to be associated with evidence of positive selection. These findings shed new light on the manner in which HGT has shaped pathogenic bacterial genomes.
Identification of Bcl-6-dependent follicular helper NKT cells that provide cognate help for B cell responses
Authors: Pheh-Ping Chang, Patricia Barral, Jessica Fitch, Alvin Pratama, Cindy S Ma, Axel Kallies, Jennifer J Hogan, Vincenzo Cerundolo, Stuart G Tangye, Robert Bittman, Stephen L Nutt, Robert Brink, Dale I Godfrey, Facundo D Batista, Carola G Vinuesa
Journal: Nature Immunology (2011)
Kinetic study of T lymphocytes after sensitization against soluble antigen. III. Potentiation and suppression of the PHA response by antigen-activated lymphocytes of low density.
Authors: J A Bash, H G Durkin, B H Waksman
Journal: The Journal of Experimental Medicine (1975)

Lymph node cells of ovalbumin-sensitized rats were separated on the basis of buoyant density into fractions reciprocally enriched in cells responsive to ovalbumin or phytohemagglutinin (PHA). Recombination of high density and low density fractions in varying proportions resulted in potentiation or suppression of the DNA synthetic response to PHA in culture. The response of cultures containing equal numbers of high and low density cells was markedly greater than the sum of the two populations stimulated separately. However, when decreasing numbers of low density cells were cultured with a constant number of high density cells, profound suppression was observed.
Isotretinoin: A Word of Caution
Authors: Frank W. Yoder
Journal: JAMA (1983)

<h3>To the Editor.—</h3> I wish to express my concern and anxiety over the potential tragedy that might arise from abuse and misuse of isotretinoin (Accutane), which has recently been approved by the Food and Drug Administration for marketing for the treatment of severe recalcitrant cystic acne. I have been closely associated with this medication since the first clinical study was performed at the National Institutes of Health (NIH), Bethesda, Md.<sup>1</sup>Its benefits for severe cystic acne and many keratinizing disorders cannot be questioned<sup>1,2</sup>; however, the potential toxicity of this drug has been seriously underemphasized. After my departure from the NIH in 1977, I continued a clinical study to evaluate isotretinoin in patients with keratinizing disorders. Of seven patients whose conditions were greatly improved with oral isotretinoin therapy, five have recently experienced symptoms and/or radiological evidence of skeletal hyperostosis. Although these patients have received a higher dose (3 mg/kg) for
Problems with the GP contract date back to 2004
Authors: H. J. Beerstecher
Journal: BMJ (2012)

The time for action on the GP contract was in 2004.1 In exchange for quick and easy cash from the Quality and Outcomes Framework (QOF), the core General Medical Services (GMS) contract was left vulnerable to unilateral imposed change, did not specify core duties, and did not contain inflation proofing. …
Space-Heater Hazards
Authors: Edwin W. Brown
Journal: New England Journal of Medicine (1961)

ON November 22, 1959, at about 3 a.m., the sixteen-year-old daughter of a Boston laborer awoke to discover that the kerosene-fueled space heater in the bedroom of their tenement apartment was ablaze. In addition to the father and the teen-age daughter, the room was occupied by 8 younger siblings. She shouted to her father, snatched 2 of the children from their beds, and carried them to safety. Returning moments later, she was barely able to drag 2 others through the door when the heater exploded, instantly converting the room into a raging inferno. The father leaped to safety through a . . .