tbuckley/cabot-search huggingface dataset:
Abstract 3471: Gene expression profiling of a single laser capture microdissected (LCM) cell
Authors: Shirley Chu
Journal: Cancer Research (2014)

Abstract Laser Capture Microdissection (LCM) is a technique providing a rapid and reliable method to procure purified cell populations, either as multiple single cells or a group of cells, from a heterogenic tissue sample allowing a targeted approach to genomic profiling. When analyzing gene expression profiles from a group of cells, the average profile may not be a true representation of the many different profiles that could exist even in a pure cell population (e.g., in different states of growth, differentiation, or activation). As a result, the transcriptional variability of individual cells and any insight into the relationship between specific genes in single cells are lost. To fully understand the complexity of tissue and cellular heterogeneity, it is necessary to measure molecular signatures at the single cell resolution. Here we developed a workflow for gene expression profiling of a single LCM cell that can also be applied to small number of LCM cells. The ArcturusXT™ LCM system was used to harvest single cells from frozen human tumor tissue sample which were then processed for quantitative RT-PCR (qRT-PCR) gene expression analysis. Citation Format: Shirley Chu. Gene expression profiling of a single laser capture microdissected (LCM) cell. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3471. doi:10.1158/1538-7445.AM2014-3471
Giverny, February 1923
Authors: JEFFREY I. COHEN
Journal: Annals of Internal Medicine (1980)

Ad Libitum1 November 1980Giverny, February 1923JEFFREY I. COHENJEFFREY I. COHENSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-93-5-777 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTomorrow, Dr. Coutela arrivesTo transplant me from my gardens at GivernyTo a bed in the city hospital.My dear friend Monsieur ClemenceauHas assured me that all will go well.I am only certain that when I returnI will have left behind the cloudy lensesFloating inside my headIn exchange for a pair of spectacles.I have waited long enough.I have seen my arms weakenMy stature decreaseWhile my canvases grow larger each day.My brushes lengthen, the bristles multiplyUntil their stroke is as wide and unwieldyAs an old man's arm.I... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: JEFFREY I. COHENAffiliations: Johns Hopkins University School of Medicine Baltimore, Maryland PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 November 1980Volume 93, Issue 5 Page: 777-777 Keywords Stroke ePublished: 1 December 2008 Issue Published: 1 November 1980 PDF downloadLoading ...
Integrating Palliative Care for Liver Transplant Candidates
Authors: Amy J. Markowitz, Stephen J. McPhee
Journal: JAMA (2006)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Prediction of the Localization of High-Risk Coronary Atherosclerotic Plaques on the Basis of Low Endothelial Shear Stress
Authors: Yiannis S. Chatzizisis, Michael Jonas, Ahmet U. Coskun, Roy Beigel, Benjamin V. Stone, Charles Maynard, Ross G. Gerrity, William Daley, Campbell Rogers, Elazer R. Edelman, Charles L. Feldman, Peter H. Stone
Journal: Circulation (2008)

Low endothelial shear stress (ESS) promotes the development of atherosclerosis; however, its role in the progression of atherosclerotic plaques and evolution to inflamed high-risk plaques has not been studied. Our hypothesis was that the lowest values of ESS are responsible for the development of high-risk coronary atherosclerotic plaques associated with excessive expansive remodeling.Twenty-four swine, treated with streptozotocin to induce diabetes and fed a high-fat diet, were allocated into early (n=12) and late (n=12) atherosclerosis groups. Intima-media thickness was assessed by intravascular ultrasound in the coronary arteries at weeks 4 and 8 in the early group and weeks 23 and 30 in the late group. Plaques started to develop after week 8, leading to marked heterogeneity in plaque severity at week 30. ESS was calculated in plaque-free subsegments of interest (n=142) in the late group at week 23. Coronary arteries (n=31) of this group were harvested at week 30, and the subsegments of interest were identified and analyzed histopathologically. Low ESS was an independent predictor of the development of high-risk plaques, characterized by intense lipid accumulation, inflammation, thin fibrous cap, severe internal elastic lamina degradation, and excessive expansive remodeling. The severity of high-risk plaque characteristics at week 30 was significantly correlated with the magnitude of low ESS at week 23.The magnitude of low ESS determines the complexity and heterogeneity of atherosclerotic lesions and predicts the development of high-risk plaque.
Lateral Aberrant Thyroid
Authors: F. T. Crossling
Journal: BMJ (1962)

Lateral Aberrant Thyroid Sir,?Drs. R. W. P. Johnson and N. C. Saha have once again brought up the interesting point about the existence of the lateral aberrant thyroid (June 16, p. 1668), and, with the histological finding of neoplasia as they describe in their cases, I am sure most clinicians would agree with their interpretation. I think it is important, however, to clearly specify the background context of neoplasia in this connexion, as I feel that without it the use of the term  lateral aberrant thyroid  is liable to be rather ambiguous or misleading. The authors state in their summary that the term can be abandoned, but this surely suggests that lateral aberrant thyroid tissue?not necessarily malignant?does not exist as an entity. This is certainly not so, as is illustrated by the following case. Mrs. E. Y., aged 66, was seen with a complaint of a swelling in the right side of her neck which she had noticed for about eight months. She had also been generally tired, and had observed slight huskiness of her voice. There was no cough, sputum, or dyspnoea. Examination showed a picture of myxoedema and obesity. There was a discrete firm swelling in the right posterior triangle of neck just lateral to the junction of the middle and lower thirds of sternomastoid, about 2 cm. in diameter. This was quite distinct and separate from the thyroid gland, which was also found to be uniformly firm in consistence but not appreci ably enlarged. She was admitted to the surgical unit of Mr. John Hutchison for further investigation. AT-ray of her chest suggested some enlargement of the right hilum. Bronchoscopy, however, showed no endobronchial abnormality. In view of the suspicion that the cervical swelling might be a metastatic deposit from bronchus or thyroid, an excision biopsy was performed. Subsequent histology revealed thyroid tissue with appearances not unlike those of Hashimoto's disease. At least three different serological tests were then tried to confirm this diagnosis, but all were negative for Hashimoto's disease. We were so impressed at the time by the discovery that practically all references to lateral aberrant thyroid were in connexion with the finding of malignant thyroid tissue that, together with our failure to get serological confirmation of Hashimoto's disease, we felt obliged to explore the thyroid gland itself. A right hemithyroidectomy was therefore performed, and this established an irrefutable histological diagnosis of Hashimoto's disease with no evidence of neoplasia. Thyroid extract was prescribed for the woman, who has done very well since then.
SOME MASTOID INSTRUMENTS.AND SOME APPLIANCES FOR EAR WORK.
Authors: FRANK ALLPORT
Journal: JAMA (1906)

<h3>A MIDDLE EAR SYRINGE.</h3> Practically all middle ear syringes are unclean and frequently out of order. I submit one of glass ano rubber that can always be ready for use, and which is so cheap that the surgeon can afford to possess a number of them (Fig. 1). An objection has been raised to it that the point is not large enough to enter minute perforations of the drumhead. In answer to this I contend that no middle ear should be irrigated through a minute drumhead perforation. Such tympanic irrigations should be confined to those cases where the irrigating liquid can find a ready exit, otherwise dangerous complications are likely to ensue. <h3>A MASTOID PERIOSTEOTOME.</h3> In stripping the mastoid process of its periosteum after the primary incision has been accomplished, an effort should be made to preserve all possible periosteum. This purpose is liable to be defeated by the sharp
A Competing Risk Analysis for Hospital Length of Stay in Patients With Burns
Authors: Sandra L. Taylor, Soman Sen, David G. Greenhalgh, MaryBeth Lawless, Terese Curri, Tina L. Palmieri
Journal: JAMA Surgery (2015)

IMPORTANCE Current outcome predictors for illness and injury are measured at a single time point-admission.However, patient prognosis often changes during hospitalization, limiting the usefulness of those predictions.Accurate depiction of the dynamic interaction between competing events during hospitalization may enable real-time outcome assessment.OBJECTIVE To determine how the effects of burn outcome predictors (ie, age, total body surface area burn, and inhalation injury) and the outcomes of interest (ie, mortality and length of stay) vary as a function of time throughout hospitalization. DESIGN, SETTING, AND PARTICIPANTSIn this retrospective study, we used the American Burn Association's National Burn Repository, containing outcomes and patient and injury characteristics, to identify 95 579 patients admitted with an acute burn injury to 80 tertiary American Burn Association burn centers from 2000 through 2009.We applied competing risk statistical methods to analyze patient outcomes. MAIN OUTCOMES AND MEASURESWe estimated the cause-specific hazard rates for death and discharge to assess how the instantaneous risk of these events changed across time.We further evaluated the varying effects of patient age, total body surface area burn, and inhalation injury on the probability of discharge and death across time.RESULTS Maximum length of stay among patients who died was 270 days and 731 days among those discharged.Total body surface area, age, and inhalation injury had significant effects on the subdistribution hazard for discharge (P < .001);these effects varied across time (P < .002).Burn size (coefficient -0.046) determined early outcomes, while age (coefficient -0.034) determined outcomes later in the hospitalization.Inhalation injury (coefficient -0.622) played a variable role in survival and hospital length of stay. CONCLUSIONS AND RELEVANCEReal-time measurement of dynamic interrelationships among burn outcome predictors using competing risk analysis demonstrated that the key factors influencing outcomes differed throughout hospitalization.Further application of this analytic technique to other injury or illness types may improve assessment of outcomes.
Abstract 2821: GRK6 deficiency promotes tumor aggressiveness and metastasis in a murine model of human lung cancer .
Authors: Natalie Sutton, Nikia Smith, Ariel J. Thomas, Sandeep K. Raghuwanshi, Ricardo M. Richardson
Journal: Cancer Research (2013)

Abstract G protein coupled receptor kinases (GRKs) phosphorylate the activated form of G protein coupled receptors (GPCRs) followed by the association of β-arrestin (βarr) with the phosphorylated receptor, desensitization and down-regulation. We have recently shown that the chemokine receptor, CXCR2, couples to GRK6 to regulate cellular responses including chemotaxis, angiogenesis and wound healing. In this study, we sought to investigate the role of GRK6 in CXCR2-mediated tumorigenesis using a murine model of human lung cancer. Mice deficient in GRK6 (GRK6−/−) exhibited a significant increase in Lewis lung cancer (LLC) growth and metastasis relative to control littermates (GRK6+/+). GRK6 deletion had no effect on the expression of pro-angiogenic chemokine or vascular endothelial growth factor (VEGF), but up-regulated matrix metalloproteinase (MMP)-2 and MMP-9 release, tumor-infiltrating PMNs and microvessel density. Treatment of the mice with a CXCR2 inhibitor (SB225002) or a murine anti-CXCR2 antibody blocked the effect of GRK6 deletion. Surprisingly, a GRK6−/−/βarr2−/− double knockout animal model exhibited faster tumor growth relative to GRK6−/− or βarr2−/− animals. Exposure of GRK6−/−/βarr2−/− mice to the anti-CXCR2 antibody totally inhibited tumor growth. This suggest that the effects of βarr2 and GRK6 knockdown in CXCR2-mediated tumor development are additive. Altogether, the results indicate that CXCR2 couples to GRK6 to regulate angiogenesis, tumor progression and metastasis. Deletion of GRK6 increases the activity of the host CXCR2, resulting in greater PMN infiltration and MMP release in the tumor microenvironment thereby promoting angiogenesis and metastasis. Since GRK6−/−/βarr2−/− mice showed greater tumor growth relative to GRK6−/− or βarr2−/− mice, the data also suggest that CXCR2 couples to a different mechanism to mediate tumor progression and metastasis. Citation Format: Natalie Sutton, Nikia Smith, Ariel J. Thomas, Sandeep K. Raghuwanshi, Ricardo M. Richardson. GRK6 deficiency promotes tumor aggressiveness and metastasis in a murine model of human lung cancer . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2821. doi:10.1158/1538-7445.AM2013-2821
A Rare Case of Primary Paranasal Sinus Angiosarcoma With Pulmonary Metastasis Detected by 18F-FDG PET/CT
Authors: Dengjun Sun, Rong Yu, Jian Chen, Dongmei Geng, Liangming Zhang
Journal: Clinical Nuclear Medicine (2015)

The study reports a rare case of primary paranasal sinus angiosarcoma with pulmonary metastases detected by 18F-FDG PET/CT. A 29-year-old woman presented with nasal congestion and rhinorrhea for 6 months. CT scanning showed a large mass in the right maxillary sinus, which had infiltrated the surrounding tissues. Subsequent evaluation by 18F-FDG PET/CT indicated numerous nodules in the lung, in addition to the paranasal sinus mass, which exhibited elevated FDG activity. Histologic examination after nasal endoscopic biopsy confirmed the diagnosis as primary paranasal sinus angiosarcoma with pulmonary metastases.
A virologic survey of patients admitted to a critical care unit for acute cardiorespiratory failure
Authors: Fabrice Carrat, Marianne Leruez-Ville, Marc Tonnellier, Jean-Luc Baudel, Juliette Deshayes, Pascal Meyer, Eric Maury, Julie Galimand, Christine Rouzioux, Georges Offenstadt
Journal: Intensive Care Medicine (2005)
Chest Injuries: Physiologic Principles and Emergency Management
Authors: Alan Marty
Journal: JAMA (1971)

This lucid presentation of the pathophysiology, diagnosis, and treatment of chest injuries is entirely clinical in approach, by an author who has treated more than 5,000 chest-injured patients. While he provides a bibliography, he does not in the text refer to these papers. Instead, to stress an important principle, he draws on his experience or uses cases representing an error in management. For example, a patient died, expectorating and drowning in blood thought secondary to bullet wounds of the chest and head. At necroscopy, the nasopharynx was found to be the only bleeding source. Conclusion: "Simple postnasal packing would have averted death." The reader is first exposed to several excellent color illustrations by Frank Netter. A clear, practical discussion of all pertinent basic sciences follows, aimed primarily at the medical student. Standard bedside procedures such as nasotracheal suction or intercostal drainage techniques are then explained. A lengthy section on problems
United States Health Care System Reform
Authors: George D. Lundberg
Journal: JAMA (1994)

<h3>My Way</h3> If I were starting an American health care system from scratch in 1994, this is what I would do. All persons would have a primary care physician of their own choosing, who would provide comprehensive continuing care and refer as appropriate. I would emphasize prevention and early detection of disease; proven interventions, such as immunizations, Papanicolaou tests, and mammography after age 50 years, would be free to patients and paid for by government tax revenues. Specialty and subspecialty physician care would be by referral only. All ambulatory care provided by physicians and other health care professionals or organizations would be paid by patients out of pocket, up to some annual legal maximum (perhaps $3000 per person). Patients would be expected to pay their bills. Physicians and other providers would be expected to provide a reasonable amount of free care or offer reduced charges for medications, diagnostic tests, and
SCAAR: CHANGES IN DRUG ELUTING STENT USE AND INSTENT-RESTENOSIS RATES FROM 2002 TO 2012
Authors: Christoph Varenhorst, Giovanna Sarno, Kristina Hambraeus, Oskar Angers, Gran Olivecrona, Bo Lagerqvist, Stefan James
Journal: Journal of the American College of Cardiology (2014)
Role of Hyperplasia in Vascular Lesions of Cholesterol-fed Rabbits Studied with Thymidine-H <sup>3</sup> Autoradiography
Authors: Sanford C. Spraragen, Victor P. Bond, Lewis K. Dahl
Journal: Circulation Research (1962)

Rabbit aorta and coronary vessels were studied with thymidine-H 3 and autoradiography. They were found to comprise an extremely stable population of cells, the latter probably even more stable than the former. Compensatory repair by means of regeneration at sites of bifurcation seems inadequate. This in part may explain the susceptibility of these areas to the development of atherosclerotic lesions. Proliferation, although sequentially secondary to fatty infiltration, plays an active role in the pathogenesis of cellular atherosclerotic lesions as seen in the rabbit. Foam cells possess the ability to synthesize DNA even when incorporated within the atherosclerotic lesion. Arguments in support of and opposed to some of the current hypotheses for the pathogenesis of atherosclerosis have been presented.
The Lev and LeNegre Syndromes
Authors: RICHARD J. PERRY
Journal: Annals of Internal Medicine (1982)

Letters and Corrections1 December 1982The Lev and LeNegre SyndromesRICHARD J. PERRY, M.D.RICHARD J. PERRY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-97-6-932_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Confusion on the distinction between Lev's and LeNegre's syndromes of cardiac conduction defects is a persistent problem for many students of internal medicine.To review the subject, I consulted a definitive reference, the new edition ofThe Heartedited by Hurst and associates (1), which recently was reviewed in this journal by D.I. Peretz (1982;97:470-1). I found only a single reference to LeNegre's disease after the chapter on "Recognition of Arrhythmias and Conduction Abnormalities" by Henry J.L. Marriott and Robert J. Myerburg. Apparently the authors were unable to clarify the matter; the paper cited and attributed to...Reference1. HURSTLOGUERACKLEY JRC, eds. The Heart. Arteries and Veins. 5th ed. New York; McGraw-Hill Book Company; 1982. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Roger Williams General Hospital Providence, RI 02908 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byLev and Lenègre DiseasesGEORGE NIKOLIC, M.B., B.S. 1 December 1982Volume 97, Issue 6Page: 932-932KeywordsArrhythmiaHeart Issue Published: 1 December 1982 PDF downloadLoading ...
Complex Rib Fracture Presenting as Solitary Linear Focus of Increased Uptake on Bone Scan Imaging
Authors: KEN MORGAN, HELEN WINER-MURAM, DAN MASSIE
Journal: Clinical Nuclear Medicine (1986)

Tc-99m MDP bone imaging demonstrated linear increased uptake involving the posterior right eighth rib. A biopsy specimen showed an old rib fracture. Typically, metastatic lesions are linear, whereas fractures appear as focal, rounded areas of increased activity. Often, fractures involve multiple ribs and are linearly oriented vertically. The present case is unusual scintigraphically, but the specimen radiograph explains the variant pattern.
Modification by Beta-Adrenergic Blockade of the Circulatory Responses to Acute Hypoxia in Man*
Authors: David W. Richardson, Hermes A. Kontos, A. Jarrell Raper, John L. Patterson
Journal: Journal of Clinical Investigation (1967)

In 17 healthy men, beta-adrenergic blockade reduced significantly the tachycardia and the elevation of cardiac output associated with inhalation of 7.5% oxygen for 7 to 10 minutes. Hypoxia did not increase plasma concentrations of epinephrine or norepinephrine in six subjects. Furthermore, blockade of alpha and beta receptors in the forearm did not modify the vasodilation in the forearm induced by hypoxia, providing pharmacologic evidence that hypoxia of the degree and duration used was not associated with an increase in the concentrations of circulating catecholamines in man. Part of the increase in cardiac output and heart rate during acute hypoxia in man is produced by stimulation of beta-adrenergic receptors, probably by cardiac sympathetic nerves. The mechanism of the vasodilation in the forearm during hypoxia remains uncertain.
Scintigraphy of Discitis
Authors: GARY F. GATES
Journal: Clinical Nuclear Medicine (1977)

Four children with discitis were studied with 99mTcpolyphosphate bone scintigraphy. All showed narrowing of the intravertebral disc space with accentuated nuclide accumulation in adjacent vertebral bodies. This image pattern may be unique to discitis and when occurring in a young child with a painful back should strongly suggest that diagnosis.
Senegal outlaws female genital mutilation
Authors: J. Ciment
Journal: BMJ (1999)

Senegal has joined Burkina Faso, the Central African Republic, Djibouti, Ghana, and Togo in outlawing female genital mutilation and assigning penalties of up to five years imprisonment to those who order or perform the procedure. Currently, 20% of Senegalese women have undergone female genital mutilation, and many girls are thought to have died as a result. UN agencies have made their opposition to female genital mutilation clear and have argued that women attempting to avoid the procedure should be granted asylum in other countries.