tbuckley/cabot-search huggingface dataset:
Abstract A51: Development and initial characterization of isogenic cell lines stably overexpressing prostate cancer candidate genes
Authors: Lukasz Kacprzyk, Stephanie Blaich, Rainer Wittig, Melanie Hudler, Holger Sültmann, Jan Mollenhauer
Journal: Cancer Research (2009)

Abstract Microarray analyses of global changes in gene expression patterns have recovered a large number of genes, which are deregulated in prostate cancer, when compared to normal prostate tissue. In order to successfully translate these data into clinical intervention strategies, it is crucial to determine genes that truly contribute to tumor development (which may be referred to as ‘driver genes’) and to separate them from the majority of secondary anomalies in expression levels (‘passenger genes’). To obtain the missing functional information, we construct isogenic cell line libraries derived from commonly used prostate (RWPE-1) and prostate cancer (LNCaP, PC-3) cell lines and subject them to a range of functional assays. Using a system for rapid generation of stably-transfected isogenic cell lines, that was developed in our group, we create a prostate cancer library in a two-step procedure. At first, acceptor clones are generated from cancer and normal prostate cell lines by stable integration of a plasmid carrying a recombinase target sequence. Incorporation of a second expression plasmid with the gene of interest is then mediated by site-specific recombination. This procedure allows a facilitated transgene integration into a predefined, transcriptionally active locus. It also minimizes the influence of genetic background, as clones generated within such a library differ among each other only by the presence of the gene of interest in the same chromosomal location. Therefore, in contrast to traditional knock-in strategies, our system ensures that readily interpretable phenotypes are obtained and thereby provides a highly standardized resource for functional gene analysis. The technology is well suited for medium throughput and was designed to link data from high-throughput genomic analyses with functional gene analysis in animal models. LNCaP and PC-3 acceptor cell lines have been generated and characterized. Recombination of PC-3 with several well-described, cancer-relevant genes under the control of an inducible promoter indicated the feasibility of the method. The next step will include initial screening of the library of roughly 100 recombinants for changes in the cell viability and proliferation. The most promising effectors from the primary screen will be followed with an expanded range of functional assays, addressing their impact on cell cycle, apoptosis and cell migration. This can then serve as a starting point for an individual characterization of the novel prostate cancer genes. Citation Information: Cancer Res 2009;69(23 Suppl):A51.
THE DISTRIBUTION OF POLYMORPHIC TRAITS IN ATOPIC ASTHMATIC PATIENTS
Authors: KENJI MANO, SHOJI HARADA, HIDEO MIYAHARA, TERUMASA MIYAMOTO, YOSHIHIKO HORIUCHI
Journal: Allergy (1975)

The phenotypic distribution and gene frequencies of haptoglobin (Hp), transferrin (Tf), group specific component (Gc), cholinesterase (Cho E2), and alpha1-antitrypsin (Pi) in plasma proteins, and phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase ((6-PGD), esterase D (Es D), phosphohexose isomerase (PHI), adenosine deaminase (ADA) and acid phosphatase (AcP) in red cells were studied in 127 atopic, asthmatic patients. The gene frequencies were compared with normal groups. The phenotypic distribution of the Pi system in atopic patients was somewhat different from the normal. No significant differences were found between the two groups in protein systems or in enzyme systems, except Pi systems. In conclusion, except for the Pi system, no definite association between polymorphic traits and atopic asthma was found in this study.
Invasive Versus Noninvasive Evaluation of Coronary Artery Disease
Authors: Joanne D. Schuijf, Jacob M. van Werkhoven, Gabija Pundziute, J. Wouter Jukema, Isabel Decramer, Marcel P. Stokkel, Petra Dibbets-Schneider, Martin J. Schalij, Johannes H.C. Reiber, Ernst E. van der Wall, William Wijns, Jeroen J. Bax
Journal: JACC. Cardiovascular imaging (2008)
Bedside Saline-Contrast Transthoracic Echocardiography Placement of Bicaval Dual-Lumen Catheter for Venovenous Extracorporeal Membrane Oxygenation
Authors: Don Hayes, Thomas J. Preston, Patrick I. McConnell, Mark Galantowicz, Andrew R. Yates
Journal: American Journal of Respiratory and Critical Care Medicine (2013)
National Congenital Rubella Surveillance Programme 1 July 1971-30 June 1984.
Authors: R W Smithells, S Sheppard, H Holzel, A Dickson
Journal: BMJ (1985)

This is the fourth report of a series on the surveillance of congenital rubella by the National Congenital Rubella Surveillance Pro- gramme using methods previously described in the BM7.'-3 We present data on 763 children (including nine co-twins, that is, nine pairs) classified by the Northern and Southern Registries of the National Congenital Rubella Surveillance Programme as cases of confirmed or suspected congenital rubella.The children were reported between 1 July 1971 and 30 June 1984, a period during which large and moderate rubella epidemic years were interspersed among non-epidemic years (figure).The same figure shows by year
Phillip Neal Mitchell, MD
Authors: Brigitte Dayan
Journal: JAMA (1992)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Intrauterine Device In the Broad Ligament
Authors: Arthur I. Goldstein
Journal: JAMA (1972)

<h3>To the Editor.—</h3> Perforation of the uterus by a Lippes Loop has a reported incidence ranging from 0.4 to 8.1 per 1,000 insertions.1-3 Most are asymptomatic and the loops are either free or adherent to the omentum.<sup>4</sup>We present a case in which an intrauterine device (IUD) was incidentally found in the broad ligament. Report of a Case.—This 28-year-old woman was admitted for elective sterilization. Approximately four years earlier she had a cervical conization because of a class 3 Papanicolaou smear. Three years prior to admission, a second conization specimen was reported as chronic cervicitis with focal areas of dysplasia. All subsequent Pap smears have been negative. Approximately two years ago, an IUD was inserted. A year later the patient became pregnant. A therapeutic abortion was performed and the IUD was supposedly removed. Since that time, the patient has had a completely normal menstrual pattern. Clinical Course.—During the course of a
Role of EGF Receptor Activation in Angiotensin II–Induced Renal Epithelial Cell Hypertrophy
Authors: Jianchun Chen, Jian-Kang Chen, Eric G. Neilson, Raymond C. Harris
Journal: Journal of the American Society of Nephrology (2006)

For determination of the molecular mechanisms underlying the induction of epithelial cell hypertrophy by angiotensin II (Ang II), a well-characterized porcine renal proximal tubular cell line LLCPKcl4, which does not express endogenous Ang II receptor subtypes, was transfected with cDNA encoding Ang II subtype 1 receptor (AT1R/Cl4). Ang II transactivated the EGF receptor (EGFR) in these AT1R/Cl4 cells, which was blocked by the selective AT1R antagonist losartan but not by the selective AT2R antagonist PD123319. Ang II did not transactivate EGFR in empty vector-transfected LLCPKcl4 cells (Vector/Cl4). Ang II elicited release of soluble heparin-binding EGF-like growth factor (HB-EGF) from AT1R/Cl4 cells, and Ang II-induced EGFR activation was prevented by pretreatment with the specific HB-EGF inhibitor CRM197 or the metalloproteinase inhibitors batimastat or phenanthroline, none of which had any effect on EGFR activation by exogenously administered EGF. Ang II stimulated protein synthesis and cell hypertrophy in AT1R/Cl4 cells without increasing cell number, and signaling studies revealed that Ang II stimulated phosphorylation of the 40S ribosomal protein S6 and the eukaryotic translation initiation factor 4E-binding protein 1, the two downstream target proteins of the mammalian target of rapamycin, which is a central regulator of protein synthesis and cell size. Ang II-induced mammalian target of rapamycin activation, [3H]leucine incorporation, and cellular hypertrophy were inhibited by pretreatment with either batimastat or CRM197 or by pretreatment with rapamycin or the EGFR tyrosine kinase inhibitor AG1478. Ang II also stimulated Smad 2/3 phosphorylation, which was blocked by a selective TGF-beta receptor I kinase inhibitor but not by CRM197. With blockade of TGF-beta receptor, Ang II-mediated hypertrophy was converted into cell proliferation, which was blocked by CRM197. In summary, this is the first demonstration that HB-EGF shedding-dependent EGFR transactivation, along with activation of TGF-beta signaling pathways, mediates Ang II-induced renal tubular epithelial cell hypertrophy.
Foreign Medical Graduates and Graduate Medical Education
Authors: Louis J. Goodman
Journal: JAMA (1981)

Since 1975 the flow of foreign medical graduates (FMGs) into US graduate medical education programs has been declining as a result of several factors, primarily because of the more stringent entrance requirements mandated by the 1976 Health Professions Educational Assistance Act (PL 94-484). From 1975 to 1979 the percentage of FMGs in US residencies has declined from 28.5% to 20.4%, a decline of 20.5%. Even more dramatic was the 73.4% decline in exchange visitor FMG residents. Partially offsetting these declines were the increases registered by US medical graduates and US citizen FMGs, resulting in fewer unfilled approved residency positions during the four-year period. However, it is doubtful if these increases are sufficient to meet replacement needs, especially in several metropolitan areas, specialties, and hospitals. The waiver of certain requirements of PL 94-484 may need to be extended to aid transition to decreased dependence of FMGs.
1001-24 Long-term follow-up of safety and feasibility of autologous skeletal myoblast transplantation in patients undergoing coronary artery bypass graft surgery
Authors: Nabil Dib, Dean Kereiakes, Patrick McCarthy, Jonathan Dinsmore, W.Robert MacLellan, Gregg Fonarow, Robert Michler, Diane Buchele, Philip Binkley, Shaun Opie, Amir Gahremanpour, Sean Kane, Marwan Ghazoul, Edward Diethrich
Journal: Journal of the American College of Cardiology (2004)
An audit of the use of self-administered adrenaline syringes in patients with angio-oedema
Authors: R.A. Sabroe, A-K. Glendinning, I. Sabroe, F. Lawlor, A. Kobza Black
Journal: British Journal of Dermatology (2002)

Self-administered adrenaline syringes may be prescribed for patients at risk of life-threatening episodes of angio-oedema or anaphylaxis.To determine whether patients are able to use these syringes appropriately and adequately.Twenty-nine consecutive patients who had been prescribed self-administered adrenaline syringes for severe angio-oedema were recruited. All completed a questionnaire (unsupervised), and were asked to demonstrate how to use a dummy syringe.Three of 29 (10%) patients had been prescribed syringes in the absence of severe angio-oedema or collapse. Seventeen of 29 (59%) patients had been prescribed two syringes, and 21 of 29 (72%) kept a syringe with them at all times. Twenty of 28 (71%) patients had had the use of a syringe demonstrated to them with the initial prescription, but two of 29 (7%) had never been shown how to use it. Only six of 26 (23%) patients had been told to telephone for an ambulance after using a syringe. Only seven of 29 (24%) patients would use a syringe for an episode of collapse, whereas eight of 28 (29%) would use one for an episode of lip swelling. Nine of 21 (43%) patients had not been warned about adverse effects, although 13 of 20 (65%) given adrenaline had had at least one adverse effect. Of the 25 patients asked to demonstrate their use of a syringe, only 14 (56%) were able to perform all steps correctly, and three (12%) were unable to perform any of the steps. Despite this, all 29 patients felt confident about giving themselves an injection, and most felt more secure having been prescribed syringes.As self-administered adrenaline syringes are prescribed for life-threatening events, it is vital that they are given to appropriate patients with adequate written instructions and proper demonstration at the time of the initial prescription. As a result of this study we have developed a more detailed patient information leaflet, and all patients are shown how to use a syringe for a second time when attending the clinic for follow-up.
Cytology or Biopsy
Authors: SAMUEL R. NEWCOM
Journal: Annals of Internal Medicine (1988)

Letters and Corrections1 April 1988Cytology or BiopsySAMUEL R. NEWCOM, M.D.SAMUEL R. NEWCOM, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-108-4-639_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Bottles and associates (1) report the convenient use of fine-needle aspiration as a first step in the evaluation of lymphadenopathy in the acquired immunodeficiency syndrome (AIDS) clinic at San Francisco General hospital. For carefully selected AIDS patients, we have used this approach in our hospital as well.I wish to take issue with one point, the use of the termbiopsyto describe this preparation. The termbiopsyshould be restricted to describe the removal of tissue, that is, a collection of similar cells and their intercellular substances. A needle aspirate, by definition, disrupts tissue and frequently...REFERENCE1. BOTTLESMCPHAULVOLBERDING KLP. Fine-needle aspiration biopsy of patients with the acquired immunodeficiency syndrome (AIDS): experience in an outpatient clinic. Ann Intern Med. 1986;108:42-5. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Grady Memorial Hospital Emory University Atlanta, GA 30303 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1988Volume 108, Issue 4Page: 639-639KeywordsAIDSBiopsyCell biologyCellsHospitals Issue Published: 1 April 1988 PDF DownloadLoading ...
PASSIVE TRANSFER OF TRANSPLANTATION IMMUNITY
Authors: John S. Najarian, Joseph D. Feldman
Journal: The Journal of Experimental Medicine (1962)

Passive transfer of tritiated thymidine labeled lymphoid cells sensitized to homologous tissues into non-sensitized isologous hosts resulted in accelerated rejection of homologous skin grafts in the recipients. Despite 33 per cent label of the suspension, only rare labeled sensitized lymphoid cells could be found at the site of rejection. Passive transfer of sensitized lymphoid cells in millipore chambers implanted subcutaneously or intraperitoneally in non-sensitized isologous hosts resulted in accelerated homograft rejection in the recipients. Transfer of transplantation immunity could not be accomplished with serum from sensitized hosts. The rejection of homologous tissues without the physical presence of the sensitized cell at the graft site suggested that a humoral agent produced by the cell was capable of rejecting the homograft.
Processing of Some Antigens by the Standard Proteasome but Not by the Immunoproteasome Results in Poor Presentation by Dendritic Cells
Authors: Sandra Morel, Frédéric Lévy, Odile Burlet-Schiltz, Francis Brasseur, Michaël Probst-Kepper, Anne-Lise Peitrequin, Bernard Monsarrat, Robert Van Velthoven, Jean-Charles Cerottini, Thierry Boon, Jean Edouard Gairin, Benoît J. Van den Eynde
Journal: Immunity (2000)
Abstract P4-07-01: The Class I Selective PI3K Inhibitor GDC-0941 Enhances the Efficacy of Docetaxel in Human Breast Cancer Models by Increasing the Rate of Apoptosis
Authors: JJ Wallin, J Guan, WW Prior, LB Lee, L Ross, LD Belmont, H Koeppen, M Belvin, D Sampath, LS Friedman
Journal: Cancer Research (2012)

Abstract Docetaxel (DTX) is commonly used as a front-line treatment option for breast cancer but many patients ultimately relapse and succumb to disease progression. Phosphatidylinositol 3-kinases (PI3K) are lipid kinases that regulate breast tumor cell growth, migration and survival. Given that phosphatidylinositol 3-kinase (PI3K) is frequently activated in breast cancer and induces chemo-resistance, it is an attractive target for combination therapy with standard of care drugs such as DTX. The current study was intended to determine if GDC-0941, an orally bioavailable class I selective PI3K inhibitor, enhances the anti-tumor activity of DTX in human breast cancer models in vitro and in vivo. A panel of 25 breast tumor cell lines representing luminal, HER2+ and basal sub-types were treated with GDC-0941, DTX or the combination of both drugs and assayed for cellular viability, modulation of PI3K pathway markers and apoptosis induction. The combination of GDC-0941 and DTX decreased the cellular viability of breast tumor cell lines in vitro but with variable drug synergy. The addition of both drugs resulted in stronger synergistic effects in a sub-set of tumor cell lines that was not predicted by breast cancer sub-type or mutations within the PI3K pathway. Human xenografts of breast cancer cell lines and patient-derived tumors were utilized to assess efficacy of GDC-0941 and DTX in vivo. In these models, the best combination efficacy was detected when the two drugs were dosed within 1 hour of each other. We also observed that GDC-0941 increased the rate of apoptosis in cells arrested in mitosis upon co-treatment with DTX. Our data provides a preclinical rationale for evaluating GDC-0941 in combination with DTX for breast cancer treatment. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P4-07-01.
Gastrointestinal Bleeding in Runners
Authors: Richard I. Andron
Journal: Annals of Internal Medicine (1991)

Letters1 March 1991Gastrointestinal Bleeding in RunnersRichard I. Andron, MDRichard I. Andron, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-114-5-429 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editors: Schwartz and colleagues (1) documented occult gastrointestinal bleeding in 9 of 41 marathon runners. The source of bleeding was found in 3 of 7 patients who had endoscopy. Although all runners in the study completed a questionnaire, no mention is made of medications used during training and before the race itself.Distance runners experience many musculoskeletal injuries and frequently use nonsteroidal anti-inflammatory drugs (NSAIDs) in their treatment programs. Because the lesions found by upper endoscopy in the patients described by Schwartz and colleagues (1) are similar to those produced by NSAIDs, it would be helpful to know...Reference1. SchwartzVanagunasKamel AAP. Endoscopy to evaluate gastrointestinal bleeding in marathon runners. Ann Intern Med. 1990;113:632-3. LinkGoogle Scholar1. SchwartzVanagunasKamel AAP. Endoscopy to evaluate gastrointestinal bleeding in runners. Ann Intern Med. 1990;113:632-3. LinkGoogle Scholar2. BaskaMosesGrabberKearney RFG. Gastrointestinal bleeding during an ultramarathon. Dig Dis Sci. 1990;35:276-79. CrossrefMedlineGoogle Scholar3. GaudinZerathGuezennec KEC. Gastric lesions secondary to long-distance running. Dig Dis Sci. 1990;35:1239-43. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: 200 Engle Street Englewood, NJ 07631 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 March 1991Volume 114, Issue 5Page: 429-429KeywordsDrugsEndoscopyHemorrhageLesionsMusculoskeletal injuryNSAIDsQuestionnairesRacial and ethnic issues Issue Published: 1 March 1991 PDF DownloadLoading ...
Tumor progression locus 2/Cot is required for activation of extracellular regulated kinase in liver injury and toll-like receptor-induced TIMP-1 gene transcription in hepatic stellate cells in mice
Authors: Maria J. Perugorria, Lindsay B. Murphy, Nicola Fullard, Jayashree B. Chakraborty, Dimitra Vyrla, Caroline L. Wilson, Fiona Oakley, Jelena Mann, Derek A. Mann
Journal: Hepatology (2012)

Toll-like receptors (TLRs) function as key regulators of liver fibrosis and are able to modulate the fibrogenic actions of nonparenchymal liver cells. The fibrogenic signaling events downstream of TLRs on Kupffer cells (KCs) and hepatic stellate cells (HSCs) are poorly defined. Here, we describe the MAP3K tumor progression locus 2 (Tpl2) as being important for the activation of extracellular regulated kinase (ERK) signaling in KCs and HSCs responding to stimulation of TLR4 and TLR9. KCs lacking Tpl2 display defects with TLR induction of cytokines interleukin (IL)-1β, IL-10, and IL-23. tpl2 −/− HSCs were unable to increase expression of fibrogenic genes IL-1β and tissue inhibitor of metalloproteinase 1 (TIMP-1), with the latter being the result of defective stimulation of TIMP-1 promoter activity by TLRs. To determine the in vivo relevance of Tpl2 signaling in liver fibrosis, we compared the fibrogenic responses of wild-type (WT) and tpl2 −/− mice in three distinct models of chronic liver injury. In the carbon tetrachloride and methionine-choline–deficient diet models, we observed a significant reduction in fibrosis in mice lacking Tpl2, compared to WT controls. However, in the bile duct ligation model, there was no effect of tpl2 deletion, which may reflect a lesser role for HSCs in wounding response to biliary injury. Conclusion : We conclude that Tpl2 is an important signal transducer for TLR activation of gene expression in KCs and HSCs by the ERK pathway and that suppression of its catalytic activity may be a route toward suppressing fibrosis caused by hepatocellular injuries. (Hepatology 2013)
Europe is to legislate on cross border health care
Authors: Rory Watson
Journal: BMJ (2007)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following Europe is to legislate on cross border health care Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Efficacy of orally active chemical conjugate of low molecular weight heparin and deoxycholic acid in rats, mice and monkeys
Authors: Yong-kyu Lee, Sang Kyoon Kim, Dong Yun Lee, Seulki Lee, Choong-Yong Kim, Ho-Chul Shin, Hyun Tae Moon, Youngro Byun
Journal: Journal of Controlled Release (2006)