tbuckley/cabot-search huggingface dataset:
Wnt/β-catenin signaling is a key downstream mediator of MET signaling in glioblastoma stem cells
Authors: Kang Ho Kim, Ho Jun Seol, Eun Hee Kim, Jinguen Rheey, Hyun Jin Jin, Yeri Lee, Kyeung Min Joo, Jeongwu Lee, Do-Hyun Nam
Journal: Neuro-Oncology (2012)

Glioblastoma (GBM) is the most lethal and common type of primary brain tumor. Recent evidence suggests that a subpopulation of GBM cells (glioblastoma stem cells [GSCs]) is critical for tumor progression, invasion, and therapeutic resistance. We and others have demonstrated that MET, a receptor tyrosine kinase, positively regulates the stemness phenotype and radioresistance of GSCs. Here, we interrogated the downstream effector pathways of MET signaling in GSCs.We have established a series of GSCs and xenograft tumors derived from freshly dissociated specimens from patients with GBM and characterized a subpopulation enriched with MET activation (MET(high/+)). Through global expression profiling and subsequent pathways analysis, we identified signaling pathways that are enriched in MET(high/+) populations, one of which is Wnt/β-catenin signaling pathway. To determine molecular interaction and the biological consequences of MET and Wnt/β-catenin signaling, we used pharmacological and shRNA-mediated genetic inhibition and performed various molecular and cellular analyses, including flow cytometry, immunohistochemistry, and clonogenicity assays.We found that Wnt/β-catenin signaling is highly active in MET(high/+) cells, compared with bulk tumor cells. We also showed that Wnt/β-catenin signaling activities in GBM are directly modulated by the addition of ligand-mediated MET activation or MET inhibition. Furthermore, the ectopic expression of active-β-catenin (S37A and S45Y) rescued the phenotypic effects caused by MET inhibition.These data suggest that Wnt/β-catenin signaling is a key downstream effector of MET signaling and contributes to the maintenance of GSC and GBM malignancy.
NO chemiluminescence in exhaled air: interference of compounds from endogenous or exogenous sources
Authors: N. Binding, W. Müller, P.A. Czeschinski, U. Witting
Journal: European Respiratory Journal (2000)

Nitric oxide determination in exhaled air using chemiluminescence analysers is increasingly used, but may be affected by various other components of the air sample. The influence of several compounds originating from endogenous or exogenous sources on NO readings has been studied. Defined amounts of water vapour, carbon dioxide, acetone, heptane, acetonitrile, oxygen, nitrous oxide and enflurane were added to air samples with NO concentrations 0-250 parts per billion. Marked and significant decreases in NO readings, which strongly depend on the concentration of the respective interfering compound, were found for water vapour (0.155% per 1% relative humidity), carbon dioxide (1.97% per 1% CO2 volume/volume (v/v)) and nitrous oxide (0.608% per 1% v/v N2O). While acetone in concentrations up to 8.5% v/v had no measurable effect on NO readings, heptane and acetonitrile led to marked decreases. Oxygen in concentrations of up to 95% (v/v) had no effect on NO determination. NO readings were markedly decreased by >10% per 1% (v/v) of the anaesthetic enflurane. However, due to large variations in NO values, these decreases were not statistically significant. Furthermore, enflurane reacted with the molybdenum converter of the NO(x) analyser in use, resulting in major damage to the instrument. Eliminating, or at least considering, interferences by compounds present in exhaled air is an urgent prerequisite for reliable and precise nitric oxide determination.
Focal psychodynamic therapy, cognitive behaviour therapy, and optimised treatment as usual in outpatients with anorexia nervosa (ANTOP study): randomised controlled trial
Authors: Stephan Zipfel, Beate Wild, Gaby Groß, Hans-Christoph Friederich, Martin Teufel, Dieter Schellberg, Katrin E Giel, Martina de Zwaan, Andreas Dinkel, Stephan Herpertz, Markus Burgmer, Bernd Löwe, Sefik Tagay, Jörn von Wietersheim, Almut Zeeck, Carmen Schade-Brittinger, Henning Schauenburg, Wolfgang Herzog
Journal: The Lancet (2013)
Minimal Residual Disease–Guided Treatment Deintensification for Children With Acute Lymphoblastic Leukemia: Results From the Malaysia-Singapore Acute Lymphoblastic Leukemia 2003 Study
Authors: Allen Eng Juh Yeoh, Hany Ariffin, Elaine Li Leng Chai, Cecilia Sze Nga Kwok, Yiong Huak Chan, Kuperan Ponnudurai, Dario Campana, Poh Lin Tan, Mei Yoke Chan, Shirley Kow Yin Kham, Lee Ai Chong, Ah Moy Tan, Hai Peng Lin, Thuan Chong Quah
Journal: Journal of Clinical Oncology (2012)

To improve treatment outcome for childhood acute lymphoblastic leukemia (ALL), we designed the Malaysia-Singapore ALL 2003 study with treatment stratification based on presenting clinical and genetic features and minimal residual disease (MRD) levels measured by polymerase chain reaction targeting a single antigen-receptor gene rearrangement.Five hundred fifty-six patients received risk-adapted therapy with a modified Berlin-Frankfurt-Münster-ALL treatment. High-risk ALL was defined by MRD ≥ 1 × 10(-3) at week 12 and/or poor prednisolone response, BCR-ABL1, MLL gene rearrangements, hypodiploid less than 45 chromosomes, or induction failure; standard-risk ALL was defined by MRD ≤ 1 × 10(-4) at weeks 5 and 12 and no extramedullary involvement or high-risk features. Intermediate-risk ALL included all remaining patients.Patients who lacked high-risk presenting features (85.7%) received remission induction therapy with dexamethasone, vincristine, and asparaginase, without anthracyclines. Six-year event-free survival (EFS) was 80.6% ± 3.5%; overall survival was 88.4% ± 3.1%. Standard-risk patients (n = 172; 31%) received significantly deintensified subsequent therapy without compromising EFS (93.2% ± 4.1%). High-risk patients (n = 101; 18%) had the worst EFS (51.8% ± 10%); EFS was 83.6% ± 4.9% in intermediate-risk patients (n = 283; 51%).Our results demonstrate significant progress over previous trials in the region. Three-drug remission-induction therapy combined with MRD-based risk stratification to identify poor responders is an effective strategy for childhood ALL.
Circulating Tumor Cells Are Transcriptionally Similar to the Primary Tumor in a Murine Prostate Model
Authors: Kimberly T. Helzer, Helen E. Barnes, Laura Day, Jeanne Harvey, Paul R. Billings, Allyn Forsyth
Journal: Cancer Research (2009)

The abundance of circulating tumor cells (CTC) indicates patient prognosis. Molecular characterization of CTCs may add additional information about a patient's disease. However, currently available methods are limited by contamination with blood cells. We describe a study using a modified CTC-chip to capture CTCs from an orthotopic xenograft model. Using laser capture microscopy to collect CTCs from the chip, we compared transcripts from purified CTCs with those from primary and metastatic tissue. Transcriptional profiles showed strong concordance among primary, metastatic, and CTC sources. Moreover, cells captured on the chip were viable and could be expanded in culture. We conclude that the CTC-chip is a useful tool to further characterize animal models of cancer and that viable CTCs can be isolated and show transcriptional similarity to solid tumors.
Photodynamic Ablation of Lymphatic Vessels and Intralymphatic Cancer Cells Prevents Metastasis
Authors: Tuomas Tammela, Anne Saaristo, Tanja Holopainen, Seppo Ylä-Herttuala, Leif C. Andersson, Susanna Virolainen, Ilkka Immonen, Kari Alitalo
Journal: Science Translational Medicine (2011)

Destruction of lymphatic vessels by photodynamic therapy suppresses metastasis by eradicating both the in-transit tumor cells and their conduit to distant tissues.
'Til death do us part: a case of failed affection
Authors: D. Villarreal, R. H. Freeman
Journal: Cardiovascular Research (1995)

Journal Article 'Til death do us part: a case of failed affection Get access Daniel Villarreal, Daniel Villarreal * aDepartment of Internal Medicine, Division of Cardiology — 1E65, University of Missouri — Columbia, 1 Hospital Drive, Columbia, MO 65201, USA *Corresponding author. Search for other works by this author on: Oxford Academic PubMed Google Scholar Ronald H. Freeman Ronald H. Freeman bDepartment of Physiology, University of Missouri — Columbia, Columbia, MO, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 30, Issue 1, July 1995, Pages 27–30, https://doi.org/10.1016/S0008-6363(95)90141-8 Published: 01 July 1995 Article history Received: 05 January 1995 Accepted: 09 March 1995 Published: 01 July 1995
Mixed Whole Blood Chimerism Early After Nonmyeloablative Allogeneic Transplantation in Humans is not Associated with a Reduction in the Development of Acute or Chronic Graft Versus Host Disease
Authors: A. Sreedharan, H. Lee, S. Dlouhy, M. Yu, R.P. Nelson Jr.
Journal: Journal of Allergy and Clinical Immunology (2009)
Bioengineering Silicon Quantum Dot Theranostics using a Network Analysis of Metabolomic and Proteomic Data in Cardiac Ischemia
Authors: Folarin Erogbogbo, Jasmine May, Mark Swihart, Paras N. Prasad, Katie Smart, Seif El Jack, Dariusz Korcyk, Mark Webster, Ralph Stewart, Irene Zeng, Mia Jullig, Katherine Bakeev, Michelle Jamieson, Nikolas Kasabov, Banu Gopalan, Linda Liang, Raphael Hu, Stefan Schliebs, Silas Villas-Boas, Patrick Gladding
Journal: Theranostics (2013)

Metabolomic profiling is ideally suited for the analysis of cardiac metabolism in healthy and diseased states.Here, we show that systematic discovery of biomarkers of ischemic preconditioning using metabolomics can be translated to potential nanotheranostics.Thirty-three patients underwent percutaneous coronary intervention (PCI) after myocardial infarction.Blood was sampled from catheters in the coronary sinus, aorta and femoral vein before coronary occlusion and 20 minutes after one minute of coronary occlusion.Plasma was analysed using GC-MS metabolomics and iTRAQ LC-MS/MS proteomics.Proteins and metabolites were mapped into the Metacore network database (GeneGo, MI, USA) to establish functional relevance.Expression of 13 proteins was significantly different (p<0.05) as a result of PCI.Included amongst these was CD44, a cell surface marker of reperfusion injury.Thirty-eight metabolites were identified using a targeted approach.Using PCA, 42% of their variance was accounted for by 21 metabolites.Multiple metabolic pathways and potential biomarkers of cardiac ischemia, reperfusion and preconditioning were identified.CD44, a marker of reperfusion injury, and myristic acid, a potential preconditioning agent, were incorporated into a nanotheranostic that may be useful for cardiovascular applications.Integrating biomarker discovery techniques into rationally designed nanoconstructs may lead to improvements in disease-specific diagnosis and treatment.
An Official American Thoracic Society Research Statement: Comparative Effectiveness Research in Pulmonary, Critical Care, and Sleep Medicine
Authors: Shannon S. Carson, Christopher H. Goss, Sanjay R. Patel, Antonio Anzueto, David H. Au, Stuart Elborn, Joe K. Gerald, Lynn B. Gerald, Jeremy M. Kahn, Atul Malhotra, Richard A. Mularski, Kristin A. Riekert, Gordon D. Rubenfeld, Terri E. Weaver, Jerry A. Krishnan
Journal: American Journal of Respiratory and Critical Care Medicine (2013)

Section:ChooseTop of pageAbstract <<ContentsOverviewIntroductionDefinitionsExamples Comparing Effica...Interpreting Results of E...ConclusionsReferencesCITING ARTICLES
Inhaled Nitric Oxide Therapy in Adults
Authors: Mark J.D. Griffiths, Timothy W. Evans
Journal: New England Journal of Medicine (2005)

The therapeutic promise of nitric oxide (NO), a potent vasodilator, remains uncertain for adults, and licensed indications are restricted to pediatric practice. This review considers the biologic actions of inhaled nitric oxide, the clinical indications for its administration in adults, and an assessment of its potential therapeutic development.
Skin tests predict survival after autologous tumor cell vaccination in metastatic melanoma: Experience in 81 patients
Authors: A. Baars, A.M.E. Claessen, A.J.M. van den Eertwegh, H.E. Gall, A.G.M. Stam, S. Meijer, G. Giaccone, C.J.L.M. Meijer, R.J. Scheper, J. Wagstaff, J.B. Vermorken, H.M. Pinedo
Journal: Annals of Oncology (2000)
Ultrasound measurement of rectus femoris cross-sectional area and the relationship with quadriceps strength in COPD
Authors: J M Seymour, K Ward, P S Sidhu, Z Puthucheary, J Steier, C J Jolley, G Rafferty, M I Polkey, J Moxham
Journal: Thorax (2009)

<h3>Background:</h3> Quadriceps weakness and loss of muscle mass predict mortality in chronic obstructive pulmonary disease (COPD). It was hypothesised that a reduced quadriceps cross-sectional area could be detected by ultrasound in patients with COPD compared with healthy subjects, and that measurements relate to strength and fat-free mass (FFM). <h3>Methods:</h3> Rectus femoris muscle cross-sectional area (RF<sub>CSA</sub>) was measured by ultrasound and whole-body FFM estimated using electrical bioimpedance. Quadriceps strength was measured by maximum voluntary contraction and twitch tension (TwQ) following magnetic femoral nerve stimulation. <h3>Results:</h3> 26 healthy volunteers of mean (SD) age 63 (9) years and 30 patients with COPD of mean (SD) age 67 (9) years and percentage predicted forced expiratory volume in 1 s (FEV<sub>1</sub>) 48.0 (20.8)% with a similar FFM (46.9 (9.3) kg vs 46.1 (7.3) kg, p = 0.193) participated in the study. Mean RF<sub>CSA</sub> was reduced in patients with COPD by 25% of the mean value in healthy subjects(−115 mm<sup>2</sup>; 95% CI −177 to −54, p = 0.001) and was related to MRC dyspnoea scale score, independent of FFM or sex. Maximum voluntary contraction strength was linearly related to RF<sub>CSA</sub> in patients with COPD (r = 0.78, p&lt;0.001). TwQ force per unit of RF<sub>CSA</sub> was similar in both healthy individuals and those with COPD (mean (SD) 17 (4) g/mm<sup>2</sup> vs 18 (3) g/mm<sup>2</sup>, p = 0.657). Voluntary contraction strength per unit of RF<sub>CSA</sub> was dependent on central quadriceps activation and peripheral oxygen saturation in COPD. <h3>Conclusion:</h3> Ultrasound measurement of RF<sub>CSA</sub> is an effort-independent and radiation-free method of measuring quadriceps muscle cross-sectional area in patients with COPD that relates to strength.
Calcium intake and risk of primary hyperparathyroidism in women: prospective cohort study
Authors: J. M. Paik, G. C. Curhan, E. N. Taylor
Journal: BMJ (2012)

To examine the association between calcium intake and risk of primary hyperparathyroidism in women.Prospective cohort study.Nurses' Health Study I, which originally recruited participants from the 11 most populous states in the United States.58,354 female registered nurses enrolled in the Nurses' Health Study I aged 39-66 years in 1986 and with no history of primary hyperparathyroidism. Calcium intake was assessed every four years using semiquantitative questionnaires on food frequency.Incident primary hyperparathyroidism, confirmed by medical record review.During 22 years of follow-up, we recorded 277 incident cases of primary hyperparathyroidism. Women were divided into five equal groups, according to intake of dietary calcium. After adjusting for age, body mass index, race, and other factors, the relative risk of primary hyperparathyroidism for women in the group with the highest intake of dietary calcium was 0.56 (95% confidence interval 0.37 to 0.86, P=0.009 for trend), compared with the group with the lowest intake. The multivariable relative risk of primary hyperparathyroidism for women taking more than 500 mg/day of calcium supplements compared with no calcium supplements was 0.41 (95% confidence interval 0.29 to 0.60, P<0.001 for trend). Analyses restricted to participants with regular physical exams did not significantly change the association between calcium intake and risk of primary hyperparathyroidism.Increased calcium intake is independently associated with a reduced risk of primary hyperparathyroidism in women.
Diabetes Worsens Pulmonary Diffusion in Heart Failure, and Insulin Counteracts This Effect
Authors: Marco Guazzi, Roberto Brambilla, Stefano De Vita, Maurizio D. Guazzi
Journal: American Journal of Respiratory and Critical Care Medicine (2002)

Chronic heart failure (CHF) (hydrostatic stress) and diabetes (basal laminae thickening) share the potentiality of damaging the alveolar-capillary membrane. We investigated 15 control subjects and 3 groups of 15 patients each having type 2 diabetes (Group 1), CHF (Group 2), and diabetes and CHF (Group 3), to probe whether addition of diabetes worsens lung diffusion in CHF and whether insulin counteracts this effect. Compared with control subjects, carbon monoxide diffusing capacity (DLCO) and diffusing capacity of the alveolar-capillary membrane at rest were increasingly depressed from Group 1 through Group 3. DLCO was lower than predicted in 11 patients each in Groups 1 and 2 and in all patients in Group 3. Regular insulin (10 IU) was ineffective in CHF alone, whereas it improved DLCO and diffusing capacity of the alveolar-capillary membrane in diabetes; changes, however, were significantly greater in the patients with both diabetes and CHF (+17.6%, +27.3%) than in those with diabetes alone (+9.2%, +13.1%). Insulin did not affect lung spirometry, volumes, and hemodynamics. Thus, gas transfer is depressed in a number of patients with diabetes or CHF; comorbidity increases the frequency and extent of this disorder. Insulin facilitates diffusion in diabetes, through an influence on alveolar-capillary conductance, and its efficacy is greater in comorbidity; diabetes is more disturbing in patients with CHF and produces a synergistic rather than a simple additive effect.
TIG1 Promotes the Development and Progression of Inflammatory Breast Cancer through Activation of Axl Kinase
Authors: Xiaoping Wang, Hitomi Saso, Takayuki Iwamoto, Weiya Xia, Yun Gong, Lajos Pusztai, Wendy A. Woodward, James M. Reuben, Steven L. Warner, David J. Bearss, Gabriel N. Hortobagyi, Mien-Chie Hung, Naoto T. Ueno
Journal: Cancer Research (2013)

Inflammatory breast cancer (IBC) is the most lethal form of breast cancer, but the basis for its aggressive properties are not fully understood. In this study, we report that high tumoral expression of TIG1 (RARRES1), a functionally undefined membrane protein, confers shorter survival in patients with IBC. TIG1 depletion decreased IBC cell proliferation, migration, and invasion in vitro and inhibited tumor growth of IBC cells in vivo. We identified the receptor tyrosine kinase, Axl, as a TIG1-binding protein. TIG1 interaction stablilized Axl by inhibiting its proteasome-dependent degradation. TIG1-depleted IBC cells exhibited reduced Axl expression, inactivation of NF-κB, and downregulation of matrix metalloproteinase-9, indicating that TIG1 regulates invasion of IBC cells by supporting the Axl signaling pathway in IBC cells. Consistent with these results, treatment of IBC cells with the Axl inhibitor SGI-7079 decreased their malignant properties in vitro. Finally, TIG1 expression correlated positively with Axl expression in primary human IBC specimens. Our findings establish that TIG1 positively modifies the malignant properties of IBC by supporting Axl function, advancing understanding of its development and rationalizing TIG1 and Axl as promising therapeutic targets in IBC treatment.
Transcutaneous angioplasty of experimental aortic coarctation.
Authors: J E Lock, T Niemi, B A Burke, S Einzig, W R Castaneda-Zuniga
Journal: Circulation (1982)

A dilatable form of juxtaductal aortic coarctation was surgically created in 29 newborn lambs. Of the 17 long-term survivors, four lambs served as controls and 13 underwent transcutaneous balloon dilation angioplasty with either polyvinylchloride or polyethylene catheters after 7--10 weeks of recovery. During growth before dilation, there was little change in the systolic gradient across the coarctation (36.6-35.3 mm Hg) despite an increase in animal weight from 3.8 to 19.3 kg. This systolic gradient remained constant in undilated lambs throughout a 6-month follow-up. Dilation produced an immediate 65% increase in the diameter of the coarctation and a 68% decrease in the systolic gradient across the coarctation site. Successful dilation required very high (6--8 atmospheres) dilating pressures. This gradient relief persisted throughout a follow-up of up to 1 year. Although no late sequelae could be attributed to the angioplasty, one lamb suffered an anterior aortic tear (associated with a difficult postdilation wire passage across the dilation site), which resulted in fatal intrathoracic hemorrhage. Cross pathologic inspection demonstrated intimal and medial tears in successfully dilated lambs in the first 3 days after dilation; on late pathologic examination, the intima appeared completely healed, without evidence of aneurysm or accelerated atheroma formation, within 2 months. These results, in conjunction with previous human in vitro studies, support the hypothesis that human aortic coarctation may be a dilatable lesion, although the safe limits and optimal protocols for dilating human coarctations are not known.
Increased frequency of detection of Chlamydophila pneumoniae in asthma
Authors: G.L. Biscione, J. Corne, A.J. Chauhan, S.L. Johnston
Journal: European Respiratory Journal (2004)

Previous studies have suggested that chronic Chlamydophila pneumoniae infection may play a role in the pathogenesis of asthma. However, most studies have been based on serology and have been unable to differentiate acute from chronic infection. The present authors assessed the presence of acute and chronic C. pneumoniae infection in 74 spouse pairs, each consisting of one atopic asthmatic and one nonatopic nonasthmatic. Nasal secretions were sampled every 2 weeks from October to December and actively replicating C. pneumoniae infection was detected by specific RT-PCR. C. pneumoniae was detected in 31 out of 709 samples analysed, 23 (6.4%) were positive in 362 samples from asthmatic participants and in eight out of 347 (2.3%) samples from their normal spouses (with a significant difference in infection rates, 95% confidence interval: 4.2%, 1.2–7.2%). A total of 16 (22%) asthmatic and seven (9%) normal participants were positive at least once during the study. These data confirm that Chlamydophila pneumoniae infection is detected more frequently among asthmatic participants than normal control participants. Further studies are required to confirm whether infections are also present in the lower airway and whether Chlamydophila pneumoniae infection plays a role in disease pathogenesis.
Contribution of multiple inert gas elimination technique to pulmonary medicine. 2. Chronic pulmonary diseases: chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis.
Authors: A G Agusti, J A Barbera
Journal: Thorax (1994)

The multiple inert gas elimination technique (MIGET) was first described by Wagner et al in 1974.'It allows quantitation of the ventilation- perfusion (VA/Q) distribution and a precise analysis of the intrapulmonary and extra- pulmonary factors that govern gas exchange in humans.This review discusses how this technique has facilitated a better understanding of the mechanisms underlying gas exchange in two common chronic lung diseases -chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis.The methodo- logical aspects ofthe MIGET are not discussed, and the interested reader is referred to the first review in this series (Thorax 1994;49:815-824) or to some comprehensive reviews published recently on this subject.
The NALP3 inflammasome is involved in the innate immune response to amyloid-β
Authors: Annett Halle, Veit Hornung, Gabor C Petzold, Cameron R Stewart, Brian G Monks, Thomas Reinheckel, Katherine A Fitzgerald, Eicke Latz, Kathryn J Moore, Douglas T Golenbock
Journal: Nature Immunology (2008)