tbuckley/cabot-search huggingface dataset:
Sexually Dimorphic Patterns of Episomal rAAV Genome Persistence in the Adult Mouse Liver and Correlation With Hepatocellular Proliferation
Authors: Allison P Dane, Sharon C Cunningham, Nicole S Graf, Ian E Alexander
Journal: Molecular Therapy (2009)

Recombinant adeno-associated virus vectors (rAAVs) show exceptional promise for liver-targeted gene therapy, with phenotype correction in small and large animal disease models being reported with increasing frequency. Success in humans, however, remains a considerable challenge that demands greater understanding of host-vector interactions, notably those governing the efficiency of initial gene transfer and subsequent long-term persistence of gene expression. In this study, we examined long-term enhanced green fluorescent protein (eGFP) expression and vector genome persistence in the mouse liver after rAAV2/8-mediated gene transfer in early adulthood. Two intriguing findings emerged of considerable scientific and clinical interest. First, adult female and male mice showed distinctly different patterns of persistence of eGFP expression across the hepatic lobule after exhibiting similar patterns initially. Female mice retained a predominantly perivenous pattern of expression, whereas male mice underwent inversion of this pattern with preferential loss of perivenous expression and relative retention of periportal expression. Second, these changing patterns of expression correlated with sexually dimorphic patterns of genome persistence that appear linked both spatially and temporally to underlying hepatocellular proliferation. Observation of the equivalent phenomenon in man could have significant implications for the long-term therapeutic efficacy of rAAV-mediated gene transfer, particularly in the context of correction of liver functions showing metabolic zonation.
Association of coffee and caffeine consumption with fatty liver disease, nonalcoholic steatohepatitis, and degree of hepatic fibrosis
Authors: Jeffrey W. Molloy, Christopher J. Calcagno, Christopher D. Williams, Frances J. Jones, Dawn M. Torres, Stephen A. Harrison
Journal: Hepatology (2011)

Abstract Coffee caffeine consumption (CC) is associated with reduced hepatic fibrosis in patients with chronic liver diseases, such as hepatitis C. The association of CC with nonalcoholic fatty liver disease (NAFLD) has not been established. The aim of this study was to correlate CC with the prevalence and severity of NAFLD. Patients involved in a previously published NAFLD prevalence study, as well as additional NASH patients identified in the Brooke Army Medical Center Hepatology clinic, were queried about their caffeine intake. A validated questionnaire for CC was utilized to assess for a relationship between caffeine and four groups: ultrasound negative (controls), bland steatosis/not-NASH, NASH stage 0-1, and NASH stage 2-4. A total of 306 patients responded to the CC questionnaire. Average milligrams of total caffeine/coffee CC per day in controls, bland steatosis/not-NASH, NASH stage 0-1, and NASH stage 2-4 were 307/228, 229/160, 351/255, and 252/152, respectively. When comparing patients with bland steatosis/not-NASH to those with NASH stage 0-1, there was a significant difference in CC between the two groups ( P = 0.005). Additionally, when comparing patients with NASH stage 0-1 to those with NASH stage 2-4, there was a significant difference in coffee CC ( P = 0.016). Spearman's rank correlation analysis further supported a negative relationship between coffee CC and hepatic fibrosis (r = −0.215; P = 0.035). Conclusion: Coffee CC is associated with a significant reduction in risk of fibrosis among NASH patients. (Hepatology 2012)
RESPIRATORY MOVEMENTS OF THE BRONCHIAL TUBES.
Authors: E. FLETCHER INGALS
Journal: JAMA (1905)

Last summer while doing bronchoscopy on a child about 2 years of age for the removal of a foreign body from one of the branches of a main bronchus, my attention was arrested by the rhythmical dilatation and contraction of the main bronchi and their branches during inspiration and expiration. This movement was so great that in inspiration the diameter of the tube was sometimes more than twice as great as in expiration. Similar movements were shortly afterward observed in the trachea, also in another young child, although I had not observed these movements in three adults on whom I had done the operation. From this fact I surmised that the movements were not present in the larger bronchi of adults for the reason that they were prevented by the greater rigidity of the cartilages, but it seemed probable that they extended throughout the smaller bronchi at all ages. A
Barriers and motivators to blood and cord blood donations in young African-American women
Authors: Brenda Grossman, Andre R. Watkins, Faye Fleming, Michael R. DeBaun
Journal: American Journal of Hematology (2005)

The primary aim of this study was to assess potential barriers and motivators to blood and cord blood donation among African-American women. A telephone survey of African-American women, ages 18–30 years, in the St. Louis metropolitan area was performed. The survey was administered by trained telemarketing personnel using a Computer-Assisted Direct Interview (CADI) system. One hundred sixty-two women were surveyed. Common barriers to blood donation were inconvenience of donor sites (19%), fear of needles (16%), and too much time required to donate (15%). Potential motivators were increasing awareness of need for blood (43%), increasing the number of convenient donor locations (19%), and encouragement by spiritual leaders to have blood drives at their church (17%). Lack of awareness was the only identified barrier to cord blood donation. Most women surveyed (88%) indicated that they definitely or probably would donate cord blood. Strategies to increase the proportion of African-American blood and cord blood donations may include educating potential donors about the process and benefits of donation to particular patient populations and engaging church leadership in supporting blood and cord blood donations. Am. J. Hematol. 78:198–202, 2005. © 2005 Wiley-Liss, Inc.
Characterization of the humoral immune response in heparin-induced thrombocytopenia
Authors: Jang-Soo Suh, Mohammad I. Malik, Richard H. Aster, Gian Paolo Visentin
Journal: American Journal of Hematology (1997)

Recent reports indicate that antibodies associated with heparin-induced thrombocytopenia and thrombosis (HITP) are specific for complexes formed between heparin and the heparin-binding, platelet alpha granule protein, platelet factor 4 (PF4). As with other disorders mediated by immune complexes (IC), the characteristics of the involved immunoglobulins could affect the ability of IC to cause symptoms. We therefore studied the class, subclass, and potency of antibodies specific for heparin:PF4 complexes formed by two groups of patients: one with severe thrombocytopenia, with or without thrombosis, and a positive serotonin release assay (SRA) (Group 1) and another with mild or absent thrombocytopenia, absence of thrombosis, and a negative SRA despite having formed antibodies reactive with heparin:PF4 complexes (Group 2). IgG antibodies were more common in the Group 1 patients (100%) than in Group 2 (46%), whereas IgM antibodies were more common in Group 2 (81%) than in Group 1 (42%) (P = 0.009). About half of each group formed IgA antibodies. In each group, the IgG antibodies were predominantly IgG1 (82%); 42% were IgG3. Only one IgG2 antibody was identified in a total of 52 antibody formers. Antibodies of the IgG class were consistently of higher titer in Group 1 patients than in Group 2 patients (P < 0.001). Recent reports suggest that the H131 form of the Fc gammaRII receptor, which binds preferentially to IgG2 Fc, is found with greater than expected frequency in patients with HITP. Identification of only one IgG2 antibody among 38 antibodies of the IgG class argues against a unique role for antibodies of this subclass in the pathogenesis of HITP. The finding that titers of antibodies in Group 1 patients were a significantly higher titer than in Group 2 patients suggests that development of the full-blown HITP syndrome may require the formation of antibodies of unusually high titer.
Effects of PVC bags sterilization process on the 5-fluorouracil stability
Authors: T. Dine, F. Kahlfi, M. Duban, B. Gressier, M. Luyckx, C. Brunet, X. Meersseman, F. Goudaliez, M. Cazin, J.C. Cazin
Journal: Biomaterials (1999)
Effects of Organizational Change in the Medical Intensive Care Unit of a Teaching Hospital
Authors: Shannon S. Carson
Journal: JAMA (1996)

<h3>Objective.</h3> —To compare the effects of change from an open to a closed intensive care unit (ICU) format on clinical outcomes, resource utilization, teaching, and perceptions regarding quality of care. <h3>Design.</h3> —Prospective cohort study; prospective economic evaluation. <h3>Setting.</h3> —Medical ICU at a university-based tertiary care center. For the open ICU, primary admitting physicians direct care of patients with input from critical care specialists via consultation. For the closed ICU, critical care specialists direct patient care. <h3>Patients.</h3> —Consecutive samples of 124 patients admitted under an open ICU format and 121 patients admitted after changing to a closed ICU format. Readmissions were excluded. <h3>Main Outcome Measures.</h3> —Comparison of hospital mortality with mortality predicted by the Acute Physiology and Chronic Health Evaluation II (APACHE II) system; duration of mechanical ventilation; length of stay; patient charges for radiology, laboratory, and pharmacy departments; vascular catheter use; number of interruptions of formal teaching rounds; and perceptions of patients, families, physicians, and nurses regarding quality of care and ICU function. <h3>Results.</h3> —Mean±SD APACHE II scores were 15.4±8.3 in the open ICU and 20.6±8.6 in the closed ICU (<i>P</i>=.001). In the closed ICU, the ratio of actual mortality (31.4%) to predicted mortality (40.1%) was 0.78. In the open ICU, the ratio of actual mortality (22.6%) to predicted mortality (25.2%) was 0.90. Mean length of stay for survivors in the open ICU was 3.9 days, and mean length of stay for survivors in the closed ICU was 3.7 days (<i>P</i>=.79). There were no significant differences between periods in patient charges for radiology, laboratory, or pharmacy resources. Nurses were more likely to say that they were very confident in the clinical judgment of the physician primarily responsible for patient care in the closed ICU compared with the open ICU (41% vs 7%;<i>P</i>&lt;.01), and nurses were the group most supportive of changing to a closed ICU format before and after the study. <h3>Conclusions.</h3> —Based on comparison of actual to predicted mortality, changing from an open to a closed ICU format improved clinical outcome. Although patients in the closed ICU had greater severity of illness, resource utilization did not increase.
Magnetic Resonance Imaging and Invasive Evaluation of Development of Heart Failure in Transgenic Mice With Myocardial Expression of Tumor Necrosis Factor-α
Authors: Fatima Franco, Gail D. Thomas, Brett Giroir, Debora Bryant, M. Craig Bullock, Michael C. Chwialkowski, Ronald G. Victor, Ronald M. Peshock
Journal: Circulation (1999)

Background —Transgenic mice expressing tumor necrosis factor-α (TNF-α) in cardiac myocytes develop dilated cardiomyopathy, but the temporal progression to cardiac dysfunction is not well characterized. We asked (1) Does magnetic resonance imaging (MRI) provide a reproducible assessment of cardiac output in mice that correlates with invasive measurements obtained with thermodilution? (2) What is the time course of left ventricular (LV) remodeling in transgenic mice with myocardial expression of TNF-α? Methods and Results —Transgenic mice from 2 different lineages with differing amounts of myocardial TNF-α expression [lineage 1 (L1) and lineage 2 (L2)] and littermate controls (LC) were studied. In protocol 1, cardiac output (CO) and stroke volume (SV) were measured by MRI and thermodilution (TD) in 15 mice (3 L1, 4 L2, 8 LC). In protocol 2, 23 mice (7 L1, 8 L2, 8 LC) were scanned at 1 month of life and every 4 weeks thereafter. In both protocols, cine-MRI was performed with the use of a 1.5-T clinical system (1.5-mm slices, 195×195 μm in-plane resolution). MRI CO and SV correlated well with TD [CO TD (mL/min)=0.94*CO MRI +0.72, r =0.84; SV TD (μL)=1.01*SV MRI −1.07, r =0.94]. Serial MRI studies showed significant increase in LV mass and volumes over time and a significant decrease in ejection fraction in transgenic mice when compared with littermate controls. Compared with lineage 2, lineage 1 showed significantly larger LV mass and volumes and significantly lower ejection fraction. Conclusions —MRI assessment of cardiac function in mice correlates well with invasive measurements. Serial MRI studies in the TNF-α mouse model demonstrate that the rate of progression and severity of LV dysfunction are dependent on the degree of TNF-α overexpression.
Main left coronary artery disease. Clinical experience from 1964-1974.
Authors: M V Cohen, R Gorlin
Journal: Circulation (1975)

Obstructive lesions of the main left coronary artery (LCA) were demonstrated angiographically in 73 patients, comprising 4.3% of the total population referred to us for diagnostic evaluation of chest pain. Although there were no specific historical or clinical features which could absolutely distinguish this subgroup from the larger population of coronary artery disease patients, 81% (34/42) of the double Master's exercise tests, in which the patient achieved a heart rate of at least 110 beats/min. demonstrated greater than or equal 2 mm R-ST segmental depression. Of the total group of 73, 32 were evaluated during the six-year period from 1964 to 1971 and a preliminary report made in 1972. The diagnosis in the remaining 41 patients was established in the 1/2 year period from 1971 to 1973. The initial 32 patients were seen before the significance of a main LCA lesion was appreciated. In this subgroup there were five deaths at the time of cardiac catheterization. However, in the more recent group improved recognition of patients with possible main LCA disease prior to catheterization has led to a much lower death rate related to diagnostic catheterization. Only one of the last 41 patients undergoing coronary angiography has died. Nineteen patients were managed medically. Of this group 17 were considered to be under the same risk of death as the surgical candidates. Their mortality rate, as high. The risk of hying was 43.6% after 24 months, 51.1% after 36 months, and 73.6% after 42 months of observation. Although the initial surgical experience, using internal mammary artery implants and saphenous venin bypass grafts, was associated with a high mortality, direct revascularization surgery over the last 2 1/2 years has been accomplished with a perioperative mortality of only 6.2%. All deaths in the group of 40 patients receiving elective revasularization with saphenous vein bypass grafts occurred in the first six months following surgery. The risk of dying was 12.5% after six months of observation and was unchanged for the remainder of the follow-up period. After 21 months the difference in survival between the two groups is statistically significant (P less than 0.05). The one late death among the surgical survivors occurred four months after the operative procedure and was related to noncardiovaxcular surgical complications. The surgical survivors have been followed for an average of 27 months. Thus revascularization surgery has improved the prognosis for patients with main LCA disease. We currently advise prompt evaluation for any patient suspected of having this type of obstruction and urgent idrect revascularization surgery if this lesion is demonstrated angiographically.
A comparative study of two statistical models for the analysis of binary data from longitudinal studies.
Authors: H Origasa, J D Knoke
Journal: Environmental Health Perspectives (1990)

This study extensively compares two statistical models for the analysis of binary data from longitudinal studies. The first model was proposed by Zeger, Liang, and Self, which was abbreviated as ZLS model and another model was proposed by Origasa. The comparison focuses on both analytical and statistical view-points. The first discusses a type of the models and the second evaluates the effect from model misspecification by stimulation, assuming that the ZLS model is true.
(19) Lipid deposition in the dermis following renal transplant
Authors: David Goldin, S.O.B. Roberts, D.B. Evans
Journal: British Journal of Dermatology (1975)

Journal Article (19) Lipid deposition in the dermis following renal transplant Get access David Goldin, David Goldin Addenbrooke's Hospital, Cambridge Search for other works by this author on: Oxford Academic Google Scholar S.O.B. Roberts, S.O.B. Roberts Addenbrooke's Hospital, Cambridge Search for other works by this author on: Oxford Academic Google Scholar D.B. Evans D.B. Evans Addenbrooke's Hospital, Cambridge Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 93, Issue s11, 1 July 1975, Pages 59–61, https://doi.org/10.1111/j.1365-2133.1975.tb06801.x Published: 01 July 1975
List size, screening methods, and other characteristics of practices in relation to preventive care.
Authors: D M Fleming, M S Lawrence, K W Cross
Journal: BMJ (1985)

Twenty eight practices carried out a review of patient records for information about preventive procedures on two occasions in 1980 and 1982. We have now undertaken a survey of certain characteristics of the practices in an attempt to demonstrate features associated with effective preventive care. Significant favourable factors are a small list size, the setting up of a formal screening programme for cervical cytology and measuring blood pressure, and few registered patients in social classes IV and V. More successful practices also tend to be training practices, have principals with higher qualifications, and have developed good records organization. Opportunistic screening for cervical cytology and measuring blood pressure was not shown to be more effective than no policy of screening at all.
The tip of the iceberg
Authors: A McCartney
Journal: BMJ (1994)

MORI poll halfway through showing that most of those polled thought that the NHS would no longer exist in 10 years' time.The difficulty with this style of television debate is that the emotional response of one side-the workers in the system-overwhelms them, leading to an unseemly attempt to hold the floor by shouting.The management point of view was given more calmly and thus appealed to the moderator, who tended to give these speakers more time.However, most of their contribution consisted of jargon-for example, using a mixed economy of service to obtain quality outcomes, and value for money.No explanation was given of how either of these could be usefully measured or compared between organisations.Those who emerged with honour from this rather ragged debate included Phillip Hunt from the National Association of Health Authorities and NHS Trusts, on the side of reform.He suggested that in a controlled market system the pressures for efficiency in the public sector could be provided by the private sector competing at the margins, and that no further move to privatisation should be made.Another valuable contribution was from Professor David Hunter of the Nuffield Institute for Health, on the side of those who were attempting to make the reforms work.He pointed out that no objective evaluation
CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. VIII. CLINICAL USE OF CONCENTRATED HUMAN SERUM ALBUMIN IN SHOCK, AND COMPARISON WITH WHOLE BLOOD AND WITH RAPID SALINE INFUSION 12
Authors: A. Cournand, R. P. Noble, E. S. Breed, H. D. Lauson, E. De F. Baldwin, G. B. Pinchot, D. W. Richards
Journal: Journal of Clinical Investigation (1944)

2nd, 3rd degree burns, 0 S 44 19 3:30 835 4.9 4.
Percutaneous Transluminal Coronary Angioplasty — A Status Report
Authors: Robert I. Levy, Michael B. Mock, Vallee L. Willman, Eugene R. Passamani, Peter L. Frommer
Journal: New England Journal of Medicine (1981)

Two years ago, Grüntzig and his colleagues1 reported their initial experience with percutaneous transluminal coronary angioplasty (PTCA) in 50 patients. In that same issue, the Journal published an editorial that summarized a National Heart, Lung, and Blood Institute (NHLBI) conference convened to review preliminary information on 205 cases of PTCA and to discuss methods whereby physicians treating patients with cardiovascular disease could assist in the development and evaluation of the technique.2 The participants at that conference agreed that candidates for this new investigational procedure should have unacceptable angina inadequately controlled by maximal medical therapy; that they should have proximal, accessible, . . .
Comparative RNAi Screening Reveals Host Factors Involved in Enterovirus Infection of Polarized Endothelial Monolayers
Authors: Carolyn B. Coyne, Rebecca Bozym, Stefanie A. Morosky, Sheri L. Hanna, Amitava Mukherjee, Matthew Tudor, Kwang Sik Kim, Sara Cherry
Journal: Cell Host & Microbe (2011)
Karate Chops and Liver Lacerations
Authors: John Davis Cantwell
Journal: JAMA (1973)

<h3>To the Editor.—</h3> Karate is a fast-growing participation sport due partially to the medical emphasis on the benefits of physical fitness and the individual desire for self-protection in the face of high municipal crime rates. To the best of our knowledge, this letter describes probable complications not previously noted. <h3>Report of a Case.—</h3> A 39-year-old woman was hospitalized with abdominal pain, nausea, vomiting, and syncope. Six weeks earlier, at her second karate lesson, she received a combination of blows to the right subcostal abdominal region. Roentgenograms the following day of the ribs were normal, and she was treated symptomatically with mild analgesics. On the day of admission she vomited coffee-ground material. Her pulse rate on admission was 110, blood pressure was 90/70 mm Hg, and her temperature was 38.8 C. She had tenderness on palpation of the right anterior chest area. The liver edge was palpable 10 cm below the
Distribution of acid mucus in the bronchial mucous glands.
Authors: N Kollerstrom, P W Lord, W F Whimster
Journal: Thorax (1977)

The percentage area of the bronchial glands taking up the Alcian blue (pH 2-6) stain for acid mucin was obtained from sections from the trachea and from each generation down the inferior lingular axial bronchial pathway in five non-smokers and five cigarette smokers. The mean percentage value for the smokers (59-5%) was significantly less than for the non-smokers (74-4%). The percentage increased between successive generations from the trachea by an average of 6-5. This gradient did not differ significantly between the smoking and non-smoking groups. It was not possible to attribute these differences to any specific mechanism because many interacting variables were involved which could not be measured. Nevertheless the percentage mucin retention or storage provides an introduction to consideration of these variables and their response to cigarette smoke.