Detection of EGFR Mutations in the Plasma of Patients with Lung Adenocarcinoma for Real-Time Monitoring of Therapeutic Response to Tyrosine Kinase Inhibitors?
Authors: Marie Marcq, Audrey Vallée, Acya Bizieux, Marc G. Denis
Journal: Journal of Thoracic Oncology (2014)

In September 2012, a 74-year-old nonsmoker man was admitted to our hospital with dyspnea and general health degradation. A tumor in the upper lobe of the right lung, bilateral mediastinal lymphadenopathies, and a right pleural effusion were evident on abdominal computed tomography (CT) scan (Fig. 1B). Both pleural effusion and lung biopsies contained tumor cells presenting the p.L858R EGFR (exon 21) mutation. This alteration was also detected in the plasma sample collected before treatment (Fig. 1A). Two months after tyrosine kinase inhibitor (TKI) treatment initiation, the patient presented a significant general health degradation and weight loss (7 kg). No significant tumor reduction was seen on the CT scan, and a pleural effusion was still present (Fig. 1C). The activating EGFR mutation was present at a stable level in plasma. In addition, the p.T790M mutation (not detected in the primary tumor and in the previous plasma samples) was detected in the last sample (Fig. 1A). The patient experienced progressive pleural effusion and important clinical deterioration and died in December 2012. A 63-year-old never-smoker woman was presented with cough and weight loss in August 2012. The initial CT scan showed a large tumor in the upper lobe of the right lung (Fig. 2B) and a single bone metastasis. Lung biopsies were found to contain thyroid transcription factor-1 positive adenocarcinoma cells, harboring an exon 19 deletion of the EGFR gene. This alteration was also detected in the plasma (Fig. 2A). This patient was treated with an EGFR TKI. After 1 month, the patient reported improvement in both cough and weight. Two months after starting treatment, CT demonstrated a significant shrinkage of the lung tumor (50% objective response; Fig. 2C), and the EGFR mutation remained undetectable in plasma during 6 months (Fig. 2A). A progression of the tumor was seen on the control CT scan performed 11 months following treatment initiation (Fig. 2D). The EGFR-activating mutation simultaneously reappeared in the plasma. Liquid biopsies have recently emerged as an important source of biomarkers in clinical oncology. For instance, tumor cells circulating in blood can be used to determine the ALK (Anaplastic Lymphoma Kinase) status of patients with lung cancer,1Ilie M Long E Butori C et al.ALK-gene rearrangement: a comparative analysis on circulating tumour cells and tumour tissue from patients with lung adenocarcinoma.Ann Oncol. 2012; 23: 2907-2913Abstract Full Text Full Text PDF PubMed Scopus (140) Google Scholar and EGFR alterations can be detected in cell-free circulating tumor DNA of patients before TKI treatment.2Goto K Ichinose Y Ohe Y et al.Epidermal growth factor receptor mutation status in circulating free DNA in serum: from IPASS, a phase III study of gefitinib or carboplatin/paclitaxel in non-small cell lung cancer.J Thorac Oncol. 2012; 7: 115-121Abstract Full Text Full Text PDF PubMed Scopus (200) Google Scholar, 3Rosell R Carcereny E Gervais R et al.Spanish Lung Cancer Group in collaboration with Groupe Français de Pneumo-Cancérologie and Associazione Italiana Oncologia Toracica. Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): a multicentre, open-label, randomised phase 3 trial.Lancet Oncol. 2012; 13: 239-246Abstract Full Text Full Text PDF PubMed Scopus (4435) Google Scholar, 4Vallée A Marcq M Bizieux A et al.Plasma is a better source of tumor-derived circulating cell-free DNA than serum for the detection of EGFR alterations in lung tumor patients.Lung Cancer. 2013; 82: 373-374Abstract Full Text Full Text PDF PubMed Scopus (75) Google Scholar Bai et al.5Bai H Wang Z Chen K et al.Influence of chemotherapy on EGFR mutation status among patients with non-small-cell lung cancer.J Clin Oncol. 2012; 30: 3077-3083Crossref PubMed Scopus (177) Google Scholar recently demonstrated an effect of neoadjuvant chemotherapy on change in EGFR mutation in plasma samples. We present here the results obtained during follow-up of two patients during TKI treatment. Although in patient 1, who did not respond to TKI treatment, the EGFR mutation was detected at similar levels in all plasma samples, in patient 2 the EGFR mutation disappeared from plasma DNA during treatment response and reappeared at progression. Our data suggest that the disappearance of circulating EGFR-mutated DNA may be a marker of TKI response. Few studies have attempted to detect EGFR mutations in plasma samples from non–small-cell lung cancer patients under targeted therapy or during follow-up period. But the techniques used (microfluidic digital polymerase chain reaction6Yung TK Chan KC Mok TS Tong J To KF Lo YM Single-molecule detection of epidermal growth factor receptor mutations in plasma by microfluidics digital PCR in non-small cell lung cancer patients.Clin Cancer Res. 2009; 15: 2076-2084Crossref PubMed Scopus (358) Google Scholar; allele-specific arrayed primer extension),7Yam I Lam DC Chan K et al.EGFR array: uses in the detection of plasma EGFR mutations in non-small cell lung cancer patients.J Thorac Oncol. 2012; 7: 1131-1140Abstract Full Text Full Text PDF PubMed Scopus (40) Google Scholar which are time consuming and require expensive hardware, are not suitable for use in a routine clinical biochemistry or DNA diagnosis laboratory. In a recent report, whole exome sequencing of plasma DNA was used to assess tumor dynamics of patient with lung tumor.8Murtaza M Dawson SJ Tsui DW et al.Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA.Nature. 2013; 497: 108-112Crossref PubMed Scopus (1308) Google Scholar But this very powerful technique is not yet compatible with routine clinical practice. In our study, DNA extraction and EGFR mutation detection using the approved and efficient9Vallée A Le Loupp AG Denis MG Efficiency of the Therascreen® RGQ PCR kit for the detection of EGFR mutations in non-small cell lung carcinomas.Clin Chim Acta. 2014; 429: 8-11Crossref PubMed Scopus (40) Google Scholar Therascreen EGFR RGQ kit (Qiagen, Hilden, Germany) can be performed within 3 hours. We previously described that this procedure allowed us to detect activating EGFR mutations in plasma of advanced non–small-cell lung cancer patients before TKI treatment with a sensitivity of 94.7% and a specificity of 100%.4Vallée A Marcq M Bizieux A et al.Plasma is a better source of tumor-derived circulating cell-free DNA than serum for the detection of EGFR alterations in lung tumor patients.Lung Cancer. 2013; 82: 373-374Abstract Full Text Full Text PDF PubMed Scopus (75) Google Scholar Although promising, our data do not allow any reduction or change in the use of radiological examinations or even in the rebiopsy interest at the present time. But following confirmation of our results on a larger cohort, analysis of plasma DNA could turn out to be a useful biomarker for real-time monitoring of patients receiving EGFR TKI in routine clinical practice. This work was supported by a grant from Astra-Zeneca.