Use of the Prussian Blue Reaction in the Differential Diagnosis of Anemias
Authors: WARREN D. BOWMAN
Journal: Annals of Internal Medicine (1962)

Article1 September 1962Use of the Prussian Blue Reaction in the Differential Diagnosis of AnemiasWARREN D. BOWMAN JR., M.D.WARREN D. BOWMAN JR., M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-57-3-412 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAlthough Rath and Finch introduced the technique of staining bone marrow preparations for iron over a decade ago (1), this simple and useful procedure has not yet found wide application. The significance of the presence or absence of stainable iron, or hemosiderin, in marrow particles and normoblasts has been discussed elsewhere (2-6) and will be reviewed briefly below. The purpose of this paper is to illustrate the use of the Prussian blue reaction for hemosiderin in solving diagnostic problems commonly encountered by the internist, pathologist, and hematologist.MATERIALS AND METHODSMarrow is aspirated in the usual manner from the sternum,...References1. RATHFINCH CECA: Sternal marrow hemosiderin. J. Lab. Clin. Med. 33: 81, 1948. MedlineGoogle Scholar2. BEUTLERROBSONBUTTENWIESER EMJE: A comparison of the plasma iron, ironbinding capacity, sternal marrow iron and other methods in the clinical evaluation of iron stores. Ann. Intern. Med. 48: 60, 1958. LinkGoogle Scholar3. BEUTLERLARSHGURNEY ESECW: Iron therapy in chronically fatigued nonanemic women: a double blind study. Ann. Intern. Med. 52: 378, 1960. LinkGoogle Scholar4. BEUTLER E: The clinical evaluation of iron stores. New Engl. J. Med. 256: 692, 1957. CrossrefMedlineGoogle Scholar5. STEVENSCOLEMANFINCH ARDHCA: Iron metabolism: clinical evaluation of iron stores. Ann. Intern. Med. 38: 199, 1953. LinkGoogle Scholar6. PRATTJOHNSON PTME: Marrow stores in anemia. Arch. Intern. Med. 93: 725, 1954. CrossrefGoogle Scholar7. BOWMAN WD: Abnormal ("ringed") sideroblasts in various hematologic and non-hematologic disorders. Blood 18: 662, 1961. CrossrefMedlineGoogle Scholar8. DOUGLASDACIE ASJV: The incidence and significance of iron-containing granules in human erythrocytes and their precursors. J. Clin. Path. 6: 307, 1953. CrossrefMedlineGoogle Scholar9. HUTCHINSON HE: The significance of stainable iron in sternal marrow sections. Blood 8: 236, 1953. CrossrefMedlineGoogle Scholar10. BESSISBRETON-GORIUS MJ: Ferritin and ferruginous micelles in normal erythroblasts and hypochromic, hypersideremic anemias. Blood 14: 423, 1959. CrossrefMedlineGoogle Scholar11. BEUTLERDRENNANBLOCK EWM: The bone marrow and liver in iron deficiency anemia. J. Lab. Clin. Med. 43: 427, 1954. MedlineGoogle Scholar12. KAPLANZUELZERMOURIQUAND EWC: Sideroblasts. A study of stainable nonhemoglobin iron in marrow normoblasts. Blood 9: 203, 1954. CrossrefMedlineGoogle Scholar13. MOURIQUAND C: Le sideroblaste (etude morphologique et assai d'interpretation). Rev. Hemat. 13: 79, 1958. MedlineGoogle Scholar14. DACIESMITHWHITEMOLLIN JVMDJCDL: Refractory normoblastic anemia: a clinical and hematological study of seven cases. Brit. J. Haemat 5: 56, 1959. CrossrefMedlineGoogle Scholar15. BJORKMAN SE: Chronic refractory anemia with sideroblastic bone marrow: a study of 4 cases. Blood 11: 250, 1956. CrossrefMedlineGoogle Scholar16. BJORKMAN SE: Anaemia refractaria sideroblastica, in Eisenstoff-wechsel, ed. by Keiderling, W., Georg Thieme Verlag, Stuttgart, 1959, p. 234. Google Scholar17. HEILMEYERKEIDERLINGBELGERBERNAUER LWRH: Uber chronische refraktare anamien mit sideroblastischen Knochenmark (Anemia refractaria sideroblastica) Folia Haemat. (Frankf.) 2: 49, 1956. Google Scholar18. HEILMEYER L: The sidero-achrestic anaemias. Germ. Med. Monthly 4: 403, 1959. Google Scholar19. HAYHOEQUAGLINO FGD: Refractory sideroblastic anaemia and erythraemic myelosis: possible relationship and cytochemical observations. Brit. J. Haemat. 6: 381, 1960. CrossrefMedlineGoogle Scholar20. DACIE JV: The Haemolytic Anaemias: Congenital and Acquired. Part I. The Congenital Anaemias, 2d Ed., Grune & Stratton, Inc., New York, 1960, p. 227. Google Scholar21. VERLOOPRADEMAKER MCW: Anaemia due to pyridoxine deficiency in man. Brit. J. Haemat. 6: 66, 1960. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: WARREN D. BOWMANJR., M.D.Affiliations: Billings, MontanaFrom the Department of Internal Medicine, The Billings Clinic, Billings, Montana.Presented at the Montana-Wyoming regional meeting of The American College of Physicians, October 14, 1961.Requests for reprints should be addressed to Warren D. Bowman, Jr., M.D., The Billings Clinic, Ninth Avenue North at Broadway, Billings, Montana. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDepoteisen im KnochenmarkPyridoxine-Responsive Anemia—Prototype and Variations on the Theme 1 September 1962Volume 57, Issue 3Page: 412-418KeywordsAnemiaBeta-thalassemiaBone marrowHematology and oncologyPathologistsStainingSternum ePublished: 1 December 2008 Issue Published: 1 September 1962 PDF downloadLoading ...