tbuckley/cabot-search huggingface dataset:
Novel function of the chromosome 7 open reading frame 41 gene to promote leukemic megakaryocyte differentiation by modulating TPA-induced signaling
Authors: X Sun, B Lu, B Hu, W Xiao, W Li, Z Huang
Journal: Blood Cancer Journal (2014)

12-O-tetradecanoylphorbol-13-acetate (TPA) activates multiple signaling pathways, alters gene expression and causes leukemic cell differentiation. How TPA-induced genes contribute to leukemic cell differentiation remains elusive. We noticed that chromosome 7 open reading frame 41 (C7ORF41) was a TPA-responsive gene and its upregulation concurred with human megakaryocyte differentiation. In K562 cells, ectopic expression of C7ORF41 significantly increased CD61 expression, enhanced ERK and JNK signaling, and upregulated RUNX1 and FLI1, whereas C7ORF41 knockdown caused an opposite phenotype. These observations suggest that C7ORF41 may promote megakaryocyte differentiation partially through modulating ERK and JNK signaling that leads to upregulation of RUNX1 and FLI1. In supporting this, C7ORF41 overexpression rescued megakaryocyte differentiation blocked by ERK inhibition while JNK inhibition abrogated the upregulation of FLI1 by C7ORF41. Furthermore, we found that Y34F mutant C7ORF41 inhibited megakaryocyte differentiation. nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) was the major activator of C7ORF41 that in turn repressed NF-κB activity by inhibiting its phosphorylation at serine 536, while MAPK/ERK was the potent repressor of C7ORF41. Finally, we showed that C7ORF41 knockdown in mouse fetal liver cells impaired megakaryocyte differentiation. Taken together, we have identified the function of a novel gene C7ORF41 that forms interplaying regulatory network in TPA-induced signaling and promotes leukemic and normal megakaryocyte differentiation.
THE MORTALITY FOLLOWING OPERATIONS FOR PUS IN THE PELVIS.
Authors: HUNTER ROBB
Journal: JAMA (1903)

In the course of his everyday reading, as well as in his own operative work, the busy surgeon is always meeting with new data, so that at the end of a certain time he finds his mind loaded up with a mass of undigested knowledge, much of which is of no practical utility, or even apparently contradictory. Under these circumstances he finds it necessary to pass in review from time to time all these accumulated ideas, and to sift out the chaff from the wheat so that he can once more start with a clear field and find himself in a position to answer the questions: 1. What have I learned lately which I can utilize in improving my surgical judgment? 2. What omissions from or additions to my operative technic will give me better results in the future? Probably the most satisfactory way by which he can attain this
Transcriptome sequencing reveals CHD1 as a novel fusion partner of RUNX1 in acute myeloid leukemia with t(5;21)(q21;q22)
Authors: Hong Yao, Jinlan Pan, Chunxiao Wu, Hongjie Shen, Jundan Xie, Qinrong Wang, Lijun Wen, Qian Wang, Liang Ma, Lili Wu, Nana Ping, Yun Zhao, Aining Sun, Suning Chen
Journal: Molecular Cancer (2015)

RUNX1/AML1, which is a Runt family transcription factor critical for normal hematopoiesis, is frequently mutated or translocated in a broad spectrum of hematopoietic malignancies.We describe here the case of a 54-year-old female developed acute myeloid leukemia with a t(5;21)(q21;q22). Transcriptome sequencing identified the chromodomain-helicase-DNA-binding protein 1 gene, CHD1, as a novel partner gene of RUNX1. Furthermore, the patient was found to harbor FLT3-ITD mutation, which might collaborated with CHD1-RUNX1 in the development of acute myeloid leukemia.We have identified CHD1 as the RUNX1 fusion partner in acute myeloid leukemia with t(5;21)(q21;q22).
Results of patch testing to personal care product allergens in a standard series and a supplemental cosmetic series: An analysis of 945 patients from the Mayo Clinic Contact Dermatitis Group, 2000-2007
Authors: David A. Wetter, James A. Yiannias, Amy V. Prakash, Mark D.P. Davis, Sara A. Farmer, Rokea A. el-Azhary
Journal: Journal of the American Academy of Dermatology (2010)
Drug release from poly(acrylic acid) grafted poly(vinylidene fluoride) membrane bags in the gastrointestinal tract in the rat and dog
Authors: Tommy Tarvainen, Timo Nevalainen, Annika Sundell, Bror Svarfvar, Johanna Hyrsylä, Petteri Paronen, Kristiina Järvinen
Journal: Journal of Controlled Release (2000)
A Conversation With My Mother
Authors: David M. Eddy
Journal: JAMA (1994)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract LB-392: Loss of Rev7 causes PARP inhibitor resistance in BRCA1;p53-deficient mouse mammary tumor cells
Authors: Guotai Xu, Peter Bouwman, Janneke Jaspers, Mark Pieterse, Ariena Kersbergen, Jos Jonkers, Piet Borst, Sven Rottenberg
Journal: Cancer Research (2012)

Abstract We have previously shown that mammary tumors spontaneously arising in a genetically engineered mouse model for human BRCA1-associated breast cancer (K14Cre;Brca1F/F;p53F/F) are highly sensitive to the poly(ADP-ribose) polymerase (PARP) inhibitor olaparib due to their deficiency in homology-directed DNA repair (Rottenberg et al. PNAS 2008;105:17079-84). Despite the initial sensitivity, tumors are not eradicated and eventually develop drug resistance. In this project we aim to identify mechanisms that explain this resistance. In particular we are interested in alterations that result in restoration of DNA repair by homologous recombination (HR). For this purpose we performed a loss-of-function shRNA screen using Brca1; p53-deficient cell lines derived from a mouse mammary tumor. The CGH profile of these cell lines is highly similar to that of the original tumor. Moreover, these cells have successfully been transplanted orthotopically into syngeneic mice and they recapitulate the morphology and olaparib response of the donor tumor. In our screen we included 2000 hairpins from the Sigma Mission library which target 409 mouse genes involved in the DNA damage response. As a result we found enrichments for hairpins targeting Rev7 in olaparib-surviving colonies. This finding was validated with 3 individual hairpins which caused substantially decreased Rev7 mRNA levels. Moreover, Rev7-deficient Brca1−/−;p53−/− tumor cells are less sensitive to doxorubicin, carboplatin and cisplatin. Loss of Rev7 rescued mES cells from Brca1 deficiency and also correlated with the formation of Rad51 foci in both Brca1-deficient mES cells and Brca1−/−;p53−/− tumor cells after irradiation. We therefore think that homology-directed DNA repair is restored in Rev7-depleted Brca1−/−;p53−/− cells. We are currently confirming this finding using HR reporter assays and we are investigating whether the loss of Rev7 causes olaparib resistance in mouse tumors. Our data suggest that absence of REV7 function, like lack of 53BP1, contributes to PARP inhibitor resistance in vivo. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-392. doi:1538-7445.AM2012-LB-392
Fish Consumption and Risk of Sudden Cardiac Death
Authors: Christine M. Albert
Journal: JAMA (1998)

Context.—Dietary fish intake has been associated with a reduced risk of fatal cardiac end points, but not with nonfatal end points. Dietary fish intake may have a selective benefit on fatal arrhythmias and therefore sudden cardiac death.Objective.—To investigate prospectively the association between fish consumption and the risk of sudden cardiac death.Design.—Prospective cohort study.Setting.—The US Physicians' Health Study.Patients.—A total of 20551 US male physicians 40 to 84 years of age and free of myocardial infarction, cerebrovascular disease, and cancer at baseline who completed an abbreviated, semiquantitative food frequency questionnaire on fish consumption and were then followed up to 11 years.Main Outcome Measure.—Incidence of sudden cardiac death (death within 1 hour of symptom onset) as ascertained by hospital records and reports of next of kin.Results.—There were 133 sudden deaths over the course of the study. After controlling for age, randomized aspirin and beta carotene assignment, and coronary risk factors, dietary fish intake was associated with a reduced risk of sudden death, with an apparent threshold effect at a consumption level of 1 fish meal per week (P for trend=.03). For men who consumed fish at least once per week, the multivariate relative risk of sudden death was 0.48 (95% confidence interval, 0.24-0.96; P =.04) compared with men who consumed fish less than monthly. Estimated dietary n-3 fatty acid intake from seafood also was associated with a reduced risk of sudden death but without a significant trend across increasing categories of intake. Neither dietary fish consumption nor n-3 fatty acid intake was associated with a reduced risk of total myocardial infarction, nonsudden cardiac death, or total cardiovascular mortality. However, fish consumption was associated with a significantly reduced risk of total mortality.Conclusion.—These prospective data suggest that consumption of fish at least once per week may reduce the risk of sudden cardiac death in men.
Why Now? Use of Tetracycline in Young Children
Authors: Anne S. Yeager
Journal: JAMA (1977)

Tetracyclines are not effective against viruses, which cause 90% of upper and 50% of lower respiratory tract infections in children, nor are they the drugs of choice for the four bacteria that cause pneumonia in children.<sup>1,2</sup>Provided there is no malnutrition or underlying disease, the usual causes of bacterial respiratory infections in young children are<i>Diplococcus pneumoniae, Streptococcus pyogenes, Haemophilus influenzae</i>, or<i>Staphylococcus aureus</i>. While the toxicity of the tetracyclines in young children is not to be discounted, a more important argument against their use is that they are not the most effective drugs available against these bacteria. What remaining activity the tetracyclines have against the Enterobacteriaceae is of no benefit to the normal child afflicted with a respiratory infection. Organisms such as<i>Escherichia coli</i>are almost never the cause of the problem. Ray, Federspiel, and Schaffner report elsewhere in this issue (p 2069) that 80% of the children
Coronary thrombolysis with intravenously administered human tissue-type plasminogen activator produced by recombinant DNA technology.
Authors: F Van de Werf, S R Bergmann, K A Fox, H de Geest, C F Hoyng, B E Sobel, D Collen
Journal: Circulation (1984)

Coronary thrombolysis was induced by intravenous infusion of human tissue-type plasminogen activator (recombinant human t-PA or rt-PA) obtained by expression of the cloned gene in a mammalian cell system. Thrombolysis was detected by the appearance of reperfusion arrhythmia and confirmed by repeat angiography in anesthetized dogs with 1-hr-old thrombi of the left anterior descending coronary artery that were induced with a copper coil. Infusion of 1000 IU (10 micrograms)/kg/min intravenous rt-PA (n = 9) elicited reperfusion within 13.7 +/- 1.9 min (mean +/- SE) without producing systemic fibrinolysis or distal coronary embolization. Infusion of urokinase at the same rate elicited thrombolysis in seven of 10 dogs within an average of 19.3 +/- 2.2 min. However, distal coronary embolization occurred in two dogs and systemic fibrinolysis was observed in all. In three dogs treated with urokinase thrombolysis was obtained only with subsequent intracoronary infusion. Restoration of myocardial perfusion and metabolism assessed with positron-emission tomography was consistently noted in dogs treated with rt-PA. Thus, rt-PA, a clot-selective thrombolytic agent that does not activate the fibrinolytic system systemically and that is potentially available in large quantities, in view of its synthesis by recombinant DNA technology, offers a promising practical approach for coronary thrombolysis in patients with acute myocardial infarction.
Circulating immune complexes and monocyte Fc function in autoimmune diseases.
Authors: M Kavai, K Lukacs, I Sonkoly, K Paloczi, G Szegedi
Journal: Annals of the Rheumatic Diseases (1979)

The phagocytosis of separated and adherent monocytes of patients with systemic lupus erythematosus is subnormal as compared to controls on the basis of latex and yeast uptake. The monocytes from the same patients react with antibody-coated sheep red blood cells in a significantly higher degree than normal monocytes. There is a correlation between the percentage of reactive monocytes and the serum immune complex content. After brief treatment of patients with levamisole the phagocytic function of monocytes is restored and at the same time the circulating immune complex content is decreased.
Health Care System Chaos Should Spur Innovation: Summary of a Report of the Society of General Internal Medicine Task Force on the Domain of General Internal Medicine
Authors: Eric B. Larson
Journal: Annals of Internal Medicine (2004)

The Society of General Internal Medicine asked a task force to redefine the domain of general internal medicine. The Society believes that the chaos and dysfunction that characterize today's medical care and the challenges facing general internal medicine should spur innovation. The task force proposed the following recommendations. Remaining true to its core values and competencies, general internal medicine should stay both broad and deep, ranging from uncomplicated primary care to continuous care of patients with multiple, complex, chronic diseases. Postgraduate and continuing education should develop mastery. Wherever they practice, general internists should be able to lead teams and be responsible for the care given by their teams, embrace changes in information systems, and aim to provide most of the care required by their patients. Current financing of physician services, especially fee-for-service, must be changed to recognize the value of services performed outside the traditional face-to-face visit and give practitioners incentives to improve quality and efficiency and provide comprehensive, ongoing care. General internal medicine residency training should provide both broad and deep medical knowledge as well as mastery of informatics, management, and team leadership. General internal medicine residents should have options to tailor their final 1 to 2 years to fit their practice goals, often earning a certificate of added qualification in generalist fields. Research should expand to include practice and operations management, developing more effective shared decision making and transparent medical records and promoting the close personal connection that both doctors and patients want. The task force believes that these changes will benefit patients and the public and reenergize general internal medicine. *For a list of the members of the Society of General Internal Medicine Task Force on the Domain of General Internal Medicine, see the Appendix.
IL-21 Receptor Mutation in Humans Leads to Disseminated Cryptosporidiosis, Antibody Defects, and Abnormal B and T Cell Function
Authors: Monica G. Lawrence, Andrea Siegel, Suk See DeRavin, Eric Paul Hanson, Susan Moir, Christoph Klein, Steven M. Holland, Gulbu Uzel, Joshua D. Milner
Journal: Journal of Allergy and Clinical Immunology (2013)
Repertoire cloning of lupus anti-DNA autoantibodies.
Authors: P Roben, S M Barbas, L Sandoval, J M Lecerf, B D Stollar, A Solomon, G J Silverman
Journal: Journal of Clinical Investigation (1996)

To investigate the autoantibody repertoire associated with SLE, we have created phage display IgG Fab libraries from two clinically active SLE patients and from the healthy identical twin of one of these patients. The libraries from the lupus discordant twins were found to both include unusually large representations of the V(H)5 gene family. By panning with DNA, the SLE libraries each yielded IgG anti-double-stranded (ds) DNA autoantibodies, which are characteristic of lupus disease. These included a V(H)5 autoantibody from the affected twin, that has a targeted cluster of mutations that potentially improves binding affinity. The recovered IgG anti-dsDNA autoantibodies expressed the same idiotypes associated with the in vivo IgG anti-dsDNA response of the respective SLE donor. Heavy-light chain shuffling experiments demonstrated a case in which the in vitro creation of anti-dsDNA binding activity required restrictive pairing of a heavy chain with Vlambda light chains similar to those in circulating anti-dsDNA autoantibodies. By contrast, IgG anti-ds autoantibodies could not be recovered from the library from the healthy twin, or from shuffled libraries with heavy chains from the healthy twin. These repertoire analyses illustrate how inheritance and somatic processes interplay to produce lupus-associated IgG autoantibodies.
Circuits and Circuit Disorders: Approaches to Neuromodulation
Authors: Roger N. Rosenberg, Eva L. Feldman
Journal: JAMA Neurology (2015)

The scientific program for the symposium represents a comprehensive review of DBS for Parkinson disease since its inception and development by Dr DeLong and his colleagues, followed by comprehensive presentations of the dystonias, Tourette syndrome, obsessive-compulsive disorder, depression, and chemogenetics using designer receptors exclusively activated by designer drugs (DREADDs).Dr DeLong will review the concepts of circuits, circuit disorders, and associated neurological signs and symptoms.He will emphasize that the introduction of high-frequency DBS more than 2 decades ago, first for tremor and then for Parkinson disease, has led to a renaissance in functional stereotaxic surgery for movement disorders as well as for a wide variety of other neurological and psychiatric disorders.Dr DeLong maintains that DBS is not disease specific but rather circuit specific, since the same target may be used to treat a variety of movement disorders. 1,
Malpractice Claims: New Data and New Trends
Authors: William J. Curran
Journal: New England Journal of Medicine (1979)

SINCE the malpractice-insurance crisis of 1975, it has generally been reported that medical malpractice claims have quieted down somewhat. Data to support this impression have been lacking, however. Continued high monetary inflation in the United States in these years has generally resulted in rising insurance rates, despite any alleged reductions in numbers of claims and payments to plaintiffs.It has also been difficult to find evidence of changes in the type of claims being made against physicians during the middle years of the 1970's. Now, we have a nationwide survey of claims filed against St. Paul Fire and Marine Insurance . . .
Counselling problem drinkers in medical wards: a controlled study.
Authors: J Chick, G Lloyd, E Crombie
Journal: BMJ (1985)

Seven hundred and thirty one men admitted to medical wards were interviewed to identify problem drinkers who had not received previous treatment for alcoholism and who had some social support. One hundred and sixty one met the diagnostic criteria; 156 agreed to a follow up interview and were allocated to one of two groups. One group received a session of counselling about their drinking habits from a nurse while the other received only routine medical care. Both groups reported a reduction in alcohol consumption when interviewed 12 months later, but the counselled group had a significantly better outcome than the control group. It is concluded that systematic screening for alcohol consumption and related problems should become a routine part of medical assessment and that advice on drinking habits is effective if given before irreversible physical or psychosocial problems have developed.
S81 Lung Biopsy For Chronic Pulmonary Disease in Children
Authors: RM Greenhalgh, IE Yardley, F Child, J Bruce, GME Humphrey
Journal: Thorax (2012)

<h3>Introduction and Objectives</h3> Lung biopsy is frequently used in the management of children with chronic pulmonary disease to obtain a histological diagnosis. Previous studies have reported a high diagnostic yield and a low complication rate following lung biopsy in children. We set out to further evaluate the role of lung biopsy in this group of clinically challenging patients by reviewing our experience of this procedure. <h3>Methods</h3> A retrospective case-note review was carried out of all patients under 18 yrs who underwent a lung biopsy from 1998 to 2011. Data collected included age, mode of biopsy, histological findings, pre and post biopsy diagnosis, pre and post biopsy treatment, and complications of the procedure. <h3>Results</h3> Thirty three children (12 boys) underwent lung biopsy in the period studied. Their ages ranged from three months to 16 years (median 5 years 5 months). Five biopsies were obtained using a thoracoscopic approach, the remainder via thoracotomy. Following the procedure, 17 patients required ventilation on the intensive care unit for a median of two days. Eight children (24%) experienced a direct complication of the procedure. Seven required an intercostal drain for a pneumothorax, one a tension pneumothorax presenting six days post-operatively. The operative mortality was 12% (4/33). Three children (9%) died within 28 days of surgery, two due to progressive respiratory failure and one following pneumonia. One patient died three months post-operatively due to a persistent air leak from the biopsy site. Twenty-six (79%) biopsies provided a sample adequate for definitive histological diagnosis. In 16 (48%) children the working diagnosis and treatment was changed following lung biopsy. <h3>Conclusion</h3> Lung biopsy retains a place in the management of children with chronic pulmonary disease when there is diagnostic doubt. However it carries a significant morbidity and mortality which must be borne in mind when considering the need for histological diagnosis.
Myocardial Infarction and Other Vascular Diseases in Young Women
Authors: Hershel Jick
Journal: JAMA (1978)

We studied 83 women younger than 46 years with acute myocardial infarction (MI) and 154 controls. There was a strong positive association between MI and the following: (1) age, (2) both oral contraceptive and noncontraceptive estrogen use, (3) cigarette smoking, and (4) the presence of predisposing medical conditions, eg, past MI, hypertension, and diabetes. ABO blood type and family history of arterial disease were also positively associated with MI. Whereas the risks for idiopathic stroke and venous thromboembolism have also been shown to be increased among oral contraceptive users, there is comparatively little correlation between these two illnesses and age or smoking in young women. The present study, taken together with previously published work, provides reasonable estimates of the vascular risks associated with oral contraceptive use. (<i>JAMA</i>240:2548-2552, 1978)