tbuckley/cabot-search huggingface dataset:
The particulate superoxide-forming system from human neutrophils. Properties of the system and further evidence supporting its participation in the respiratory burst.
Authors: B M Babior, J T Curnutte, B J McMurrich
Journal: Journal of Clinical Investigation (1976)

Studies were performed to characterize the previously reported particulate O2--forming system from human neutrophils. Of eight reducing agents examined, including glutathione, ascorbic acid, and intermediates of the glycolytic and hexose monophosphate shunt pathways, only the pyridine nucleotides could serve as electron donors. At 0.1 mM pyridine nucleotide, O2- production was relatively independent of pH. The Km for NADH was approximately 0.7 mM regardless of pH, while with NADPH the Km varied from 0.02 mM at pH 6.0 to 0.3 mM at pH 7.5. The molar ratio of NADPH oxidized to O2- produced was consistent with the reaction: NADPH + 2 O2- leads to NADP+ H+; the product nucleotide was shown enzymatically to be NADP. O2- production was not inhibited by CN-, Na-, EDTA, or 1,10-phenanthroline. Particulate O2- production accounted for 35% of the oxygen taken up during the respiratory burst by an equivalent number of intact neutrophils. Greatly diminished O2- production was seen with particles prepared from cells obtained from three patients with chronic granulomatous disease, with 2.5 mM NADPH as electron donor. With 5.0 mM NADH similar observations were made with particles from two of the patients, but with this nucelotide, O2- production was only slightly reduced in the third case. The evidence available suggests that this particulate O2- -forming system is the one responsible for the respiratory burst in activated neutrophils. The relationship between this system and other O2- -forming system found in human neutrophils is discussed.
Ethics of Renal Biopsy
Authors: J. THOMAS LAMONT
Journal: Annals of Internal Medicine (1974)

Comments1 April 1974Ethics of Renal BiopsyJ. THOMAS LAMONT, M.D.J. THOMAS LAMONT, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-80-4-560_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: I would like to make several comments in reference to the article, "Occurrence and Nature of Glomerular Lesions after Group A Streptococci Infections in Children," which appeared in the October issue (Ann Intern Med79:492-499, 1973). It seems unwarranted to subject children to the risk and discomfort of a kidney biopsy for such indications as low serum CH50levels and minor urinary abnormalities. Granted that the risk of this procedure is minimal in expert hands, I wonder how children view this procedure, especially children who are essentially asymptomatic. What fraction of the patients were from ethnic minority...References1. HINGLAISGARCIA-TORRESKLEINKNECHT NRD: Long-term prognosis in acute glomerulonephritis. The predictive value of early clinical and pathological features observed in 65 patients. Am J Med 56:52-60, 1974 CrossrefMedlineGoogle Scholar2. KAPLANANTHONYCHAPMAN EBS: Epidemic acute glomerulonephritis associated with type 49 streptococcal pyoderma. I. Clinical and laboratory findings. Am J Med 48:9-27, 1970 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Gastrointestinal Unit The Massachusetts General Hospital Boston, Massachusetts 02114 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1974Volume 80, Issue 4Page: 560-560KeywordsBiopsyChildrenGroup A streptococciKidneysLesionsPediatric infectionsStreptococcal infections Issue Published: 1 April 1974 PDF DownloadLoading ...
Impact of benzene metabolites on differentiation of bone marrow progenitor cells.
Authors: R D Irons, W S Stillman
Journal: Environmental Health Perspectives (1996)

Interleukin-3 (IL-3) and granulocyte/macrophage-colony-stimulating factor (GM-CSF) are responsible for maintaining survival and stimulating growth of early dormant hematopoietic progenitor cells (HPC). These cytokines exhibit extensive overlap, with GM-CSF supporting growth and differentiation of myeloid HPC. A characteristic shared by a diverse group of leukemogens is the ability to act synergistically with GM-CSF to increase clonogenic response. Previous studies have revealed that pretreatment of murine HPC with hydroquinone (HQ) but not phenol, catechol, or trans-trans-muconaldehyde results in a selective enhancement of GM-CSF but not IL-3-mediated clonogenic response. Pretreatment of murine bone marrow cells with these agents or their metabolites in vitro results in increased numbers of HPC dividing and forming colonies in response to GM-CSF but not IL-3. The present studies explored the molecular mechanisms associated with altered cytokine response in early HPC in murine bone marrow and extended our initial observations in murine bone marrow to human bone marrow cells. HQ pretreatment of murine HPC did not induce either an up- or a down-regulation of GM-CSF receptors or any change in receptor affinity. CD34+ cells, which represent between 1 and 5% of human bone marrow, contain virtually all clonogenic stem and HPC. Pretreatment of CD34+ cells (approximately 95% purity) with HQ also results in enhanced clonogenic response with GM-CSF but not IL-3. These findings suggest that an early step in chemical leukemogenesis may involve transient alterations in the regulation of cytokine response to GM-CSF.
Alkyl-substituted silica gel as a carrier in the controlled release of dexmedetomidine
Authors: Pirjo Kortesuo, Manja Ahola, Minna Kangas, Tiina Leino, Sirpa Laakso, Lauri Vuorilehto, Antti Yli-Urpo, Juha Kiesvaara, Martti Marvola
Journal: Journal of Controlled Release (2001)
Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease.
Authors: S Romagnani, G F Del Prete, E Maggi, G Bellesi, G Biti, P L Rossi Ferrini, M Ricci
Journal: Journal of Clinical Investigation (1983)

The immunoglobulin-synthesizing activities of peripheral blood mononuclear cells from 57 untreated patients with Hodgkin's disease and 47 normal subjects were compared. Cumulative amounts of IgM and IgG synthesized and secreted by unstimulated and pokeweed mitogen-stimulated cells over a 7-d period were determined in a solid-phase radioimmunoassay. Synthesis of IgM in unstimulated cultures and of both IgM and IgG in cultures stimulated with pokeweed mitogen was markedly reduced in patients with Hodgkin's disease, whereas the mean level of the spontaneous IgG synthesis was enhanced. The degree and frequency of in vitro abnormalities were not influenced by disease stage or histology. Depression of pokeweed mitogen-induced immunoglobulin synthesis did not correlate with excessive number of monocytes and it was unaffected by removal of phagocytic cells or addition to the cultures of monocytes from normal individuals. On the other hand, monocytes isolated from blood of patients with Hodgkin's disease were even more effective than normal monocytes in supporting pokeweed mitogen-induced immunoglobulin synthesis by normal phagocyte-depleted mononuclear cells. Synthesis of both IgM and IgG induced by pokeweed mitogen remained subnormal after addition to patient B cell cultures of autologous irradiated T cells or allogeneic normal T lymphocytes. T cells from patients with Hodgkin's disease appeared at least as effective as normal T cells in helping pokeweed mitogen-induced immunoglobulin production by normal B cells. However, when normal T cells were co-cultured with B cells from patients with Hodgkin's disease, spontaneous IgG synthesis declined, whereas the addition of patient T cells to normal B cells resulted in an increase of spontaneous IgG synthesis. In patients showing depression of pokeweed mitogen-induced immunoglobulin synthesis the lymphoproliferative response and immunoglobulin synthesis stimulated by Staphylococcus aureus bacteria of the Cowan first strain, a T cell independent B cell mitogen, were also markedly reduced. These studies demonstrate impairment of immunoglobulin synthesis by cultured lymphocytes from untreated patients with Hodgkin's disease after stimulation with polyclonal B cell activators and suggest that the in vitro abnormalities may be, at least in part, the result of a preexisting in vivo activation of lymphocytes in Hodgkin's disease patients.
Street Drug Misrepresentation
Authors: Morris Seal
Journal: JAMA (1980)

<h3>To the Editor.—</h3> Recently we encountered a bizarre and most dangerous form of street drug misrepresentation. A person brought a sample to our anonymous analysis service after experiencing an untoward reaction following the ingestion of what was allegedly methaqualone. The tablet (Figure) appeared to be a Quaalude. Qualitative and quantitative analysis using thin-layer chromatography and gas-liquid chromatography revealed that the tablet contained phencyclidine hydrochloride (PCP) in a dose of 3 mg. Deception and misrepresentation involving street drugs are not uncommon. Caffeine is sold as an amphetamine, local anesthetics are sold as cocaine, LSD is sold as mescaline, and so on. However, it is rare when a dissociative anesthetic is sold as a sedative-hypnotic. Interestingly, low-dose phencyclidine intoxication is similar to alcohol intoxication,<sup>1</sup>which is similar to methaqualone intoxication. So, it seems that there is a method to the madness of the manufacturer of the fake methaqualone. Unfortunately, many users
WHENCE THE MONOCYTES?
Authors: HERMAN E. KATTLOVE
Journal: Annals of Internal Medicine (1969)

Letters and Comments1 February 1969WHENCE THE MONOCYTES?HERMAN E. KATTLOVE, M.D.HERMAN E. KATTLOVE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-70-2-420_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editor: In their paper, "Agranulocytosis with Monohistiocytosis Associated with Ampicillin Therapy," Drs. Graf and Tarlov (1) fail to note recent studies on the origin of the monocyte that would explain the monocytosis seen in recovery from agranulocytosis as well as its protective effect. This work, reviewed by Leder (2), suggests that the monocyte arises from the promyelocyte and is the primary source of inflammatory macrophages.Therefore, one could propose that the following occurs in recovery from agranulocytosis: It is a common observation that the myeloid series appears to regenerate in successive stages of increasing maturity so that early...References1. GRAFTARLOV M#: Agranulocytosis with monohistiocytosis associated with ampicillin therapy. Ann. Intern. Med. 69: 91, 1968. LinkGoogle Scholar2. LEDER L: The origin of blood monocytes and macrophages. Blut 16: 36, 1967. CrossrefGoogle Scholar3. CRONKITEFLIEDNERBONDRUBINIBRECHERQUASTLER EPTMVPJRGH: Dynamics of hemopoietic proliferation in man and mice studied by H3-thymidine incorporation into DNA. Ann. NY Acad. Sci. 77: 803, 1959. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: HERMAN E. KATTLOVE, M.D.Affiliations: Hematology Department Montefiore Hospital and Medical Center Bronx, N. Y. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byFe Iron 1 February 1969Volume 70, Issue 2Page: 420-420KeywordsMonocytes ePublished: 1 December 2008 Issue Published: 1 February 1969 PDF downloadLoading ...
Role of thymic and bursal lymphocyte subclasses in chronic allergic synovitis in the chicken.
Authors: D.C Dumonde, C.M Oates, R.N Maini, L.N Payne
Journal: Annals of the Rheumatic Diseases (1973)

9) Authors will be notified (i) when an abstract is received, (ii) when it is either accepted or rejected, (iii) when it is placed on a programme for a particular meeting.
The recombinant 65-kD heat shock protein of Mycobacterium bovis Bacillus Calmette-Guerin/M. tuberculosis is a target molecule for CD4+ cytotoxic T lymphocytes that lyse human monocytes.
Authors: T H Ottenhoff, B K Ab, J D Van Embden, J E Thole, R Kiessling
Journal: The Journal of Experimental Medicine (1988)

Since little is known about Tc cells in the human immune response to intracellular parasites, we have studied the role of Tc cells in response to M. bovis Bacillus Calmette-Guerin (BCG). Donors whose PBMC responded to BCG, purified protein derivative (PPD), and the recombinant 65-kD heat shock protein (HSP) of BCG generated BCG/PPD-specific CD4+ effector T lymphocytes that lysed PPD as well as recombinant 65-kD-pulsed monocytes. Nonpulsed or irrelevant antigen-pulsed target cells were lysed to a much lower but still significant extent. PPD-stimulated effector lymphocytes of a recombinant 65-kD nonresponder lysed PPD but not recombinant 65-kD-pulsed monocytes. Recombinant 65-kD-educated effector lymphocytes lysed both recombinant 65-kD- and PPD-pulsed monocytes. In addition, these effector cells efficiently lysed nonpulsed target cells. These results demonstrate that in recombinant 65-kD responders, the recombinant 65-kD HSP of BCG is an immunodominant target as well as a triggering molecule for BCG/PPD-specific CD4+ cytotoxic T cells that lyse autologous monocytes. The implications of these findings with respect to the role of the 65-kD HSP in autoimmunity are discussed.
Motor Accident Mortality and Compulsory Inspection of Vehicles
Authors: Victor R. Fuchs
Journal: JAMA (1967)

The relation between motor accident mortality and compulsory vehicle inspection is examined by regressing age-standardized mortality ratios on inspection and other variables across states. The adjusted coefficient of multiple determination is typically about 0.85. Inspection is negatively related to mortality, but the net effect of inspection is less than one third as large as the gross difference between states that require inspection and those that do not and does not generally differ from zero at high levels of statistical significance. The effect of inspection appears to be greater in states with more than one annual inspection. It is greater in low income than in high income states, and greater in high education than in low education states. Dollar estimates of the benefits of inspection are found to exceed estimated costs; the difference, however, is not as large as is often alleged.
Intraocular Tumors: A Text and Atlas
Authors: Jay I. Perlman
Journal: JAMA (1992)

This text is an updated version of Dr Jerry Shields'<i>Diagnosis and Management of Intraocular Tumors</i>, published by CV Mosby in 1983. The organization is similar to the original but with much needed revisions in clinical diagnosis, management, and prognosis. In addition, where necessary, emphasis on more modern diagnostic procedures, such as magnetic resonance imaging and high-resolution computed tomographic scanning, has been added to the discussion and photographs. The revised text significantly differs from the old in its expanded coverage of retinoblastomas and melanocytic tumors, intraocular malignancies very familiar to the authors in their oncology practice at the Wills Eye Hospital. A chapter on current understanding of the genetics of retinoblastoma is concise and well written and is a much-needed contribution on the disease and its prognosis for patients and families. The chapters are well organized. Each begins with a detailed table of contents and ends with a concise summary
Abstract 762: Genome-wide mapping of binding sites for the DEK oncoprotein by ChIP-seq.
Authors: Carl Sanden, Linnea Järvstråt, Tove Ullmark, Björn Nilsson, Urban Gullberg
Journal: Cancer Research (2013)

Abstract The DEK oncogene is overexpressed in a wide range of cancers, including acute myeloid leukemia (AML). While DEK was initially described as a protein binding to certain topological chromatin structures, recent studies implicate DEK in the epigenetic and transcriptional regulation of specific genes. To assess the global gene binding of DEK, we performed chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). We demonstrate that DEK does not bind evenly throughout the genome, but rather accumulates around transcription start sites, with binding to promoters and initial parts of the coding sequences. Furthermore, gene set enrichment analysis revealed that DEK preferentially binds genes associated with epigenetic regulation of gene expression. Our results represent the first genome-wide characterization of DEK binding, and further establish DEK as a transcriptional regulator with a specific set of target genes. Citation Format: Carl Sanden, Linnea Järvstråt, Tove Ullmark, Björn Nilsson, Urban Gullberg. Genome-wide mapping of binding sites for the DEK oncoprotein by ChIP-seq. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 762. doi:10.1158/1538-7445.AM2013-762
CDC91L1 (PIG-U) is a newly discovered oncogene in human bladder cancer
Authors: Zhongmin Guo, Jürgen F. Linn, Guojun Wu, Sarah L Anzick, Claus F Eisenberger, Sarel Halachmi, Yoram Cohen, Alexey Fomenkov, Mohammad Obaidul Hoque, Kenji Okami, Gabriel Steiner, James M Engles, Motonabu Osada, Chulso Moon, Edward Ratovitski, Jeffrey M Trent, Paul S Meltzer, William H Westra, Lambertus A Kiemeney, Mark P Schoenberg, David Sidransky, Barry Trink
Journal: Nature Medicine (2004)
US journal finds lower risk from diet pills
Authors: D. Josefson
Journal: BMJ (1997)

An independent survey conducted by the Wall Street Journal (31 October) has found a lower prevalence of cardiac valve abnormalities in people who took the drugs phentermine and fenfluramine (“phen-fen”) than previously reported.

These prescription diet pills were approved for short term treatment of obesity over 20 years ago. Twenty million Americans are obese, and excess weight is an independent risk factor for various illnesses such as hypertension, heart disease, kidney disease, and cancer.

Recently they have been prescribed for prolonged periods in patients who were only mildly overweight. Eighteen million prescriptions were written for the drugs …
Quinidine-Digoxin Interaction
Authors: NICHOLAS H.G. HOLFORD
Journal: Annals of Internal Medicine (1980)

Letters and Corrections1 October 1980Quinidine-Digoxin InteractionNICHOLAS H.G. HOLFORD, M.SC.NICHOLAS H.G. HOLFORD, M.SC.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-93-4-638 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: The paper by Leahey and colleagues (1) documenting the specificity of the interaction between digoxin and quinidine is a valuable addition to our knowledge of this phenomenon. I believe, however, that their interpretation of the mechanism of the interaction is erroneous. The observations are not in dispute: Quinidine does produce a rapid rise in digoxin concentration that is sustained in both normal volunteers and patients. The studies of Hager and coworkers (2) and Powell and associates (3) clearly show a reduction in the volume of distribution. The sustained elevation of digoxin levels, at steady state, must nevertheless...References1. LEAHEYREIFFELGIARDINABIGGER EJEJ. The effect of quinidine and other oral antiarrhythmic drugs on serum digoxin: a prospective study. Ann Intern Med. 1980;92:605-8. LinkGoogle Scholar2. HAGERFENSTERMAYERSOHN WPM. Digoxin-quinidine interaction: pharmacokinetic evaluation. N Engl J Med. 1979;300:1238-41. CrossrefMedlineGoogle Scholar3. POWELLFENSTERHAGERGRAVESWANDELLCONRAD JPWPMK. Quinidine-digoxin interaction. N Engl J Med. 1980;302:176-7. MedlineGoogle Scholar4. HOLFORD N. The quinidine-digoxin interaction. N Engl J Med. 1980;302:864. Letter. CrossrefMedlineGoogle Scholar5. HOOYMANSHOLFORDMASSIEWITTPLUYMMERKUS PNBMBF. Quinidine decreases both renal and metabolic clearance of digoxin. Am J Cardiol. 1980;45:453. Abstract. CrossrefGoogle Scholar6. DOERING W. Quinidine-digoxin interaction: pharmacokinetics, underlying mechanism and clinical implications. N Engl J Med. 1979;301:400-4. CrossrefMedlineGoogle Scholar1. CUCINELLPERL SW. Application of clearance and volume of distribution to the plateau principle of drugs. J Pharm Sci. 1970;59:1423-7. CrossrefMedlineGoogle Scholar2. ROWLANDBENETGRAHAM MLG. Clearance concepts in pharmacokinetics. J Pharmacokinet Biopharm. 1973;1:123-36. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: University of California Medical Center San Francisco, CA 94143 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByThe influence of quinidine and other agents on digitalis glycosides 1 October 1980Volume 93, Issue 4Page: 638-639KeywordsAttentionSpecificity Issue Published: 1 October 1980 PDF DownloadLoading ...
THE EFFECTS OF THE INTRATRACHEAL ADMINISTRATION OF FOREIGN SERUM
Authors: F. S. Jones
Journal: The Journal of Experimental Medicine (1924)

It has been possible to sensitize guinea pigs by the intratracheal administration of small amounts of horse or cow serum. The degree of sensitiveness is comparable to that obtained when the same doses of serum are injected into the peritoneal cavity. Nevertheless, relatively small amounts of foreign protein are slowly absorbed through the lining membranes of the lower respiratory tract. In sensitive animals it has not been possible to produce shock by intratracheal injection of amounts of serum which injected into the peritoneal cavity of other animals of the same series sensitized in the same manner always produced shock. Considerably larger doses administered intratracheally may give rise to anaphylactic symptoms. It is possible that the force necessary to inject the larger amounts of serum may have led to injury and more rapid absorption.
ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF HUMAN REOVIRUS-LIKE AGENT OF INFANTILE GASTROENTERITIS
Authors: RobertH. Yolken, HyunWha Kim, Thomas Clem, RichardG. Wyatt, AnthonyR. Kalica, RobertM. Chanock, AlbertZ. Kapikian
Journal: The Lancet (1977)
Cost Effectiveness of Cardiac Resynchronization Therapy in the Comparison of Medical Therapy, Pacing, and Defibrillation in Heart Failure (COMPANION) Trial
Authors: Arthur M. Feldman, Gregory de Lissovoy, Michael R. Bristow, Leslie A. Saxon, Teresa De Marco, David A. Kass, John Boehmer, Steven Singh, David J. Whellan, Peter Carson, Audra Boscoe, Timothy M. Baker, Matthew R. Gunderman
Journal: Journal of the American College of Cardiology (2005)
953 CHARACTERIZATION OF RESISTANCE MUTATIONS SELECTED IN VITRO BY THE NON-NUCLEOSIDE HCV POLYMERASE INHIBITORS ABT-333 AND ABT-072
Authors: G. Koev, R. Mondal, J. Beyer, T. Reisch, S. Masse, W. Kati, D. Hutchinson, C. Flentge, J. Randolph, P. Donner, A. Krueger, R. Wagner, P. Yan, T. Lin, C. Maring, A. Molla
Journal: Journal of Hepatology (2009)