tbuckley/cabot-search huggingface dataset:
Abstract 2673: In silico design of E2F1 mimics as potential anticancer agents
Authors: John E. Kerrigan, Emine E. Abali, Debabrata Banerjee, Yi-Ching Hsieh, Xiaoqi Xie, Joseph R. Bertino
Journal: Cancer Research (2010)

Abstract Introduction. We report the construction of a homology model of the human E2F1-DP1 transcription factor complex with DNA to be used as a tool for design of drugs targeted to the promoter of this oncogene based on interference of transcription factor binding. Using shape-based docking and force field calculations, we investigate the development of novel penetratin-based (PED) peptides, obtained by phage display, (E2F1 mimics) as potential binders to the major groove of GC-rich regions of DNA. Computational Procedures. The homology model of E2F1-DP1 complex with DNA was built using the Modeller (9v5) program. We used the DNA binding domains of the E2F1 and DP1 sequences (NCBI accession #'s AAC50719 and NP009042 respectively), and the x-ray crystal structure of the E2F4-DP2 complex with DNA (1CF7.pdb) as the primary template. The DNA from the crystal structure was modeled in place using the default spatial constraints in Modeller. The PED based peptide models were built using the x-ray crystal structure of the antennapedia homeodomain-DNA complex (9ANT.pdb) as a template. The alignment is shown below (The penetratin portion is shown in bold and the variable portion is in italics.). _aln.pos 10 20 30 40 50 60 9antA RQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHALSLTERQIKIWFQNRRMKWKK—–EN brt1 ————————————–RQIKIWFQNRRMKWKKHHHRLSH _consrvd **************** The PatchDock program was used to dock the peptides to the DNA structure from 1CF7.pdb based on shape complementarity. The best shape scoring major-groove binding orientation was selected and refined via energy minimization using the Amber ff99SB force field. Interaction energy scores were computed from the non-bonded energy terms of the Amber force field (Eint = ∑ Evdw + ∑ Eelec). Data Summary. The HHHRLSH sequence is predicted to form a more stable complex compared to the GGGALSA sequence. The PED-HHHRLSH peptide was shown to have potent activity against several cancer cell lines (Xie, et al. AACR 2009). In the case of the HHHRLSH sequence, the model predicts enhanced stability in going from L-Arg (−646.0 kcal/mol) to D-Arg (−688.0 kcal/mol). Conclusions. The α3 helix of E2F1 composed of KRRIYDITNVLEGI binds to the major groove of GC-rich DNA. The PED-HHHRLSH peptide complex with DNA (−646.0 kcal/mol) has similar interaction energy to that of the E2F1-DNA complex (−653.0 kcal/mol), is a stable α-helical structure, and is a potential E2F1 mimic. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2673.
Endoscopic Treatment of Postoperative Fistulas Resistant to Conservative Management Using Biological Fibrin Glue
Authors: L. R. Rábago, N. Ventosa, J. L. Castro, J. Marco, N. Herrera, F. Gea
Journal: Endoscopy (2002)

Postoperative fistulae occur frequently in standard surgical practice, but there is no general agreement on how to treat them. We summarize here our experience with endoscopic treatment.Postoperative digestive fistulae resistant to conservative treatment, in 15 patients, are retrospectively reviewed. Our series included two internal fistulas: (one rectovesical, and one high-output pleuroesophagic), and 13 external fistulas (one low-output gastrocutaneous, two low-output esophagocutaneous, seven low-output enterocutaneous, and three high-output enterocutaneous). After failure of conservative treatment, the fistulas were endoscopically located and 2 - 4 ml of reconstituted fibrin glue, Tissucol 2.0 at 37 degrees C, was injected through a catheter.The mean age of the patients was 61.2 years (38 - 86), and 60 % were men. Of the fistulas, 26.6 % were of the high-output type. The mean healing time was 16 days (5 - 40), and a mean of 2.5 sessions per patient were required (1 - 5). Complete sealing of fistulas was achieved in 86.6 % of cases; (87.5 % of the low-output and internal fistulas, and 55 % of the high-output fistulas). After follow-up ranging between 2 months and more than 3 years, only one of the sealed fistulas reopened. No complications were encountered. Overall mortality was 13.3 % (two out of 15), but in only one patient was this related to clinical deterioration because of the persistence of the fistula.We think that conservative treatment should not be prolonged beyond 14 days and that endoscopic treatment should be performed at that stage. Endoscopic sealing treatment achieves a very high success rate, without complications and at a lower cost. It could probably reduce the hospital stay, and avoid some unnecessary surgical interventions. Appropriate multicenter randomized trials are needed to confirm these results.
Letter: Vaccination of smallpox contacts.
Authors: G Bousfield, G Dick
Journal: BMJ (1973)

had a good initial response to tetracosactrin and those who did not, nor indeed between those with a low level of circulating plasma cortisol and those with a normal level.It is interesting, however, that the one patient who did show signs of a major complication during the attempted substitution had both a low level of plasma cortisol and a very poor response to tetracosactrin.Another observation which we feel is worth making is that all our patients' asthma was well controlled on a maintenance dosage of iprednisone before we attempted substitution with beclomethasone dipro- pionate.We have found that it is extremely difficult, if not impossible, to make this substitution if the asthma is not weU con- trolled initially by corticosteroids admiinistered other than 'by inihalation.-Weare, etc.,
How should we balance individual and population benefits of statins for preventing cardiovascular disease?
Authors: A. D. Hingorani, H. Hemingway
Journal: BMJ (2010)

US and UK groups revising recommendations on primary prevention of cardiovascular disease will have to decide whether to concentrate on high risk individuals or the whole population. <b>Aroon Hingorani</b> and <b>Harry Hemingway </b>argue that the evidence favours a population approach
996 MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL CRITERIA OF PERIPHERAL BLOOD HAEMATOPOIETIC STEM CELLS AUTO-TRANSPLANTATION EFFICIENCY IN PATIENTS WITH CHRONIC ALCOHOLIC HEPATITIS
Authors: A. Gumerova, A. Kiassov, I. Gazizov, T. Yilmaz, S. Abdulkhakov, M. Titova, D. Andreeva, M. Kaligin
Journal: Journal of Hepatology (2009)
Setting the TONE for Ending the Hypertension Epidemic
Authors: Jeremiah Stamler
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Conservative management of mechanical neck pain: systematic overview and meta-analysis
Authors: P. D Aker, A. R Gross, C. H Goldsmith, P. Peloso
Journal: BMJ (1996)

To review the efficacy of conservative management of mechanical neck disorders.Published and unpublished reports were identified through computerised and manual searches of bibliographical databases, reference lists from primary articles, and letters to authors, agencies, foundations, and content experts. Selection criteria were applied to blinded articles, and selected articles were scored for methodological quality. Effect sizes were calculated from raw pain scores and combined by using meta-analytic techniques when appropriate.Twenty four randomised clinical trials met the selection criteria and were categorised by type of intervention: nine used manual treatments; 12 physical medicine methods; four drug treatment; and three education of patients (four trials investigated more than one form of intervention). The intervention strategies were summarised separately. Pooling of studies was considered only within each category. Five of the nine trials that used manual treatment in combination with other treatments were combined. One to four weeks after treatment the pooled effect size was -0.6 (95% confidence interval -0.9 to -0.4), equivalent to an improvement of 16 (6.9 to 23.1) points on a 100 point scale. Sensitivity analyses on study quality, chronicity, and data imputation did not alter this estimate. For other interventions, studies could not be combined to arrive at pooled estimates of effect.There is little information available from clinical trials to support many of the treatments for mechanical neck pain. In general, conservative interventions have not been studied in enough detail to assess efficacy or effectiveness adequately.
Selection of Patients With Chronic Obstructive Pulmonary Disease for Long-term Oxygen Therapy
Authors: Richard M. Timms, Paul A. Kvale, Nicholas R. Anthonisen, C. Thomas Boylen, David W. Cugell, Thomas L. Petty, George W. Williams
Journal: JAMA (1981)

The Nocturnal Oxygen Therapy Trial, a study of oxygen therapy in patients with chronic obstructive pulmonary disease, has combined the experience of six centers concerning the selection of patients for oxygen treatment. Forty-five percent of hypoxic patients initially selected for the study improved enough during the one month of outpatient observation to allow suspension of plans to treat them with oxygen. Therefore, long-term oxygen therapy plans should only be made without a month of careful observation. Resting Pao<sub>2</sub>values less than 40 mm Hg suggest instability or that chronic obstructive pulmonary disease is not the only cause of the hypoxemia. Nasal prong oxygen flow of 1 to 3 L/min reversed hypoxemia in 95% of stable patients with chronic obstructive pulmonary disease. (<i>JAMA</i>1981;245:2514-2515)
Mitral Regurgitation — What Is Best for My Patient?
Authors: Catherine M. Otto, Edward D. Verrier
Journal: New England Journal of Medicine (2011)

Mitral regurgitation is common, with 80% of us having some normal valve leakage detectable on echocardiography. Mild regurgitation is well tolerated and rarely leads to overt clinical disease. However, severe regurgitation overloads the left ventricle as blood is pumped both backward across the mitral valve and forward to the systemic circulation. Over time, volume overload results in ventricular dilatation and eventual contractile dysfunction. In addition, increased left atrial pressure leads to atrial fibrillation and pulmonary hypertension.1 If untreated, these physiological changes lead to heart-failure symptoms and reduced survival. Both can be prevented by an intervention to eliminate mitral regurgitation at . . .
Combination of hTERT Knockdown and IFN-γ Treatment Inhibited Angiogenesis and Tumor Progression in Glioblastoma
Authors: Joseph George, Naren L. Banik, Swapan K. Ray
Journal: Clinical Cancer Research (2009)

The limitless invasive and proliferative capacities of tumor cells are associated with telomerase and expression of its catalytic component, human telomerase reverse transcriptase (hTERT). IFN-gamma modulates several cellular activities, including signaling pathways and cell cycle, through transcriptional regulation.Using a recombinant plasmid with hTERT siRNA cDNA, we downregulated hTERT during IFN-gamma treatment in human glioblastoma SNB-19 and LN-18 cell lines and examined whether such a combination could inhibit angiogenesis and tumor growth in nude mice. In vitro angiogenesis assay was done using coculture of tumor cells with human microvascular endothelial cells. In vivo angiogenesis assay was done using diffusion chambers under the dorsal skin of nude mice. In vivo imaging of intracerebral tumorigenesis and longitudinal solid tumor development studies were conducted in nude mice.In vitro and in vivo angiogenesis assays showed inhibition of capillary-like network formation of microvascular endothelial cells and neovascularization under dorsal skin of nude mice, respectively. We observed inhibition of intracerebral tumorigenesis and s.c. solid tumor formation in nude mice after treatment with combination of hTERT siRNA and IFN-gamma. Western blotting of solid tumor samples showed significant downregulation of the molecules that regulate cell invasion, angiogenesis, and tumor progression.Our study showed that the combination of hTERT siRNA and IFN-gamma effectively inhibited angiogenesis and tumor progression through the downregulation of molecules involved in these processes. Therefore, the combination of hTERT siRNA and IFN-gamma is a promising therapeutic strategy for controlling the growth of human glioblastoma.
Endoscopic Sphincterotomy for Choledocholithiasis: A Prospective Single-Center Study on the Short-Term and Long-Term Treatment Results in 483 Patients
Authors: S. Wojtun, J. Gil, W. Gietka, M. Gil
Journal: Endoscopy (1997)

Background and Study Aims: Endoscopic sphincterotomy (EST) is the method of choice for the treatment of choledocholithiasis. The aim of the present study was to assess the impact of various prognostic factors on the short-term and long-term results of EST.
STENTING DURING STEMI IS ASSOCIATED WITH LESS NEOINTIMAL HYPERPLASIA: A POOLED IVUS ANALYSIS FROM THE HORIZONS–AMI AND TAXUS IV, V, AND ATLAS TRIALS
Authors: Nobuaki Kobayashi, Akiko Maehara, Dominic Allocco, Bernard Witzenbichler, Stephen G. Ellis, Mark A. Turco, John A. Ormiston, Giulio Guagliumi, Songtao Jiang, Tom McAndrew, Keith D. Dawkins, Gregg W. Stone, Gary S. Mintz, Neil J. Weissman
Journal: Journal of the American College of Cardiology (2013)
Measuring Coverage in MNCH: Design, Implementation, and Interpretation Challenges Associated with Tracking Vaccination Coverage Using Household Surveys
Authors: Felicity T. Cutts, Hector S. Izurieta, Dale A. Rhoda
Journal: PLoS Medicine (2013)

In a PLOS Medicine Review, Felicity Cutts and colleagues describe the challenges facing the estimation of vaccination coverage in low- and middle-income countries using surveys and recommend ways to improve the measurement of this important public health indicator.
Shanghai bans public smoking
Authors: R. Tomlinson
Journal: BMJ (1994)

The city of Shanghai this week introduced a ban on smoking in many indoor public places in the latest attempt to halt China's rise in tobacco consumption. The Shanghai municipal government regulation came into force on 1 December, forbidding smoking in various public venues including cinemas, theatres, and karaoke rooms.

According to the preventive health department of the Shanghai Bureau of Public Health, it is the owners of the premises who will be fined under the new regulations rather than the illicit smokers themselves. Among the designated smoke free zones are big department stores, all public transport vehicles, museums, exhibition centres, sports stadiums, and hospitals. Not …
Who knew what, and when, at Mid Staffs?
Authors: P. Carter, B. Jarman
Journal: BMJ (2013)

The Francis report into the appalling failings of care at Mid Staffordshire NHS Trust has finally been published. Philip Carter and Brian Jarman explain how events unfolded

The extensive hearings of the inquiry into failings of care at Mid Staffordshire NHS Trust give perhaps the most intimate insight into the workings of the modern NHS yet glimpsed by outsiders—but it makes for dismal reading.

The line that consistently emerged throughout the inquiry was that those responsible at the trust and in the wider NHS were simply unaware of the scale and extent of the problems on the wards of Stafford Hospital—at least until the Healthcare Commission investigation of 2008-09. But a close analysis of the evidence generated by the inquiry casts doubt on this version of events. Indeed, the evidence suggests that there were ample warnings for all to see, yet they were seemingly dismissed, discounted, and disregarded.

As early as 2001, there were warning signs about the quality of clinical performance at Mid Staffs. In January, the first annual Dr Foster Hospital Guide was published, providing adjusted hospital death rates. Dr Foster uses hospital standardised mortality ratios (HSMRs) to assess hospitals—those with a score of less than 100 have fewer deaths than expected, and those with a score of more than 100 have more than expected.

The 1998-99 HSMR for Mid Staffs was significantly higher than expected, at 108. It was to be the pattern for the coming years: the HSMRs from 2001-02 to 2007-08 were all significantly high (at the 95% confidence interval level).1

Over the course of the public inquiry, the importance attached to these HSMRs would become a central source of contention. But the inquiry also uncovered many other warning signs that went seemingly unheeded.

On 3 August 2001 the chief executive of the south …
Predicting recovery from muscle strains, a new take on "inflammation" and the Goldman Dilemma
Authors: Andrew P Garnham
Journal: British Journal of Sports Medicine (2009)

Muscle strains are common. In clinical practice, we find several scenarios. Recreational athletes have concerns as to what the injury is and how long will it take to recover. Another group of patients are those who have recurrent muscle strains. Patients know what they have done (yet again), but now want to know how to prevent recurrence. A third group are competitive athletes across all sports—they want everything! Their concern centres on how quickly they can recover and how to minimise the chance of re-injury at the same time! This is especially important when finals and championships are upcoming, and at the professional level. Strategies for dealing with muscle strains are in the main time honoured rather than strongly evidence based, and it has been oft stated that there are many avenues of potential research.1

In this issue, Balius and colleagues2 ( see page 818 ) offer a sound basis for measuring the extent of injury to the rectus femoris in soccer players. This information, which should …
Cost analysis of early discharge after hip fracture.
Authors: W Hollingworth, C Todd, M Parker, J A Roberts, R Williams
Journal: BMJ (1993)

To ascertain the economic impact of an early discharge scheme for hip fracture patients.Population based study comparing costs of care for patients who had "hospital at home" as an option for rehabilitation and those who had no early discharge service available in their area of residence.District hospital orthopaedic and rehabilitation wards and community hospital at home scheme.1104 consecutively admitted patients with fractured neck of femur. 24 patients from outside the district were excluded.Cost per patient episode and number of bed days spent in hospital.Patients with the hospital at home option spent significantly less time as inpatients (mean of 32.5 v 41.7 days; p < 0.001). Those patients who were discharged early spent a mean of 11.5 days under hospital at home care. The total direct cost to the health service was significantly less for those patients with access to early discharge than those with no early discharge option (4884 pounds v 5606 pounds; p = 0.048).About 40% of patients with fractured neck of femur are suitable for early discharge to a scheme such as hospital at home. The availability of such a scheme leads to lower direct costs of rehabilitative care despite higher readmission costs. These savings accrue largely from shorter stays in orthopaedic and geriatric wards.
Demonstration of Diffuse Leptomeningeal Metastasis in a Treated Case of Medulloblastoma With F-18 FDG PET/CT
Authors: Madhavi Tripathi, Nikunj Jain, Abhinav Jaimini, Gunjan Garg, Maria M. D'souza, Rajnish Sharma, Rajesh K. Grover, Anupam Mondal
Journal: Clinical Nuclear Medicine (2009)

A 20-year-old man diagnosed as a case of desmoplastic cerebellar medulloblastoma had undergone shunting of the lateral ventricles and resection of the mass followed by postoperative irradiation to the entire neuraxis. There was evidence of residual disease in the posterior fossa on craniospinal MRI for which he had undergone cranial radiation and adjuvant chemotherapy. Two serial cranial MRI's (4 months apart) were done following this and though the lesion in the posterior fossa remained essentially the same with no evidence of progression the patient had a general decline in his ability to function and he was sent for a FDG-PET/CT study to evaluate for recurrent disease. The study revealed abnormal FDG uptake along the entire length of the thecal sac from the cervical to the lumbosacral level suggesting the possibility of metastasis. Spinal MRI with gadolinium contrast confirmed diffuse leptomeningeal metastasis. Thus FDG-PET/CT is a useful tool to evaluate for metastasis in medulloblastoma.
Superscan Pattern of F-18 Sodium Fluoride PET/CT Study in a Case of Prostate Cancer
Authors: Yuxin Li, Bashir A. Tafti, Wisam Shaba, Gholam R. Berenji
Journal: Clinical Nuclear Medicine (2011)

A 63-year-old man with a history of prostate cancer (Gleason score, 9/10) received brachytherapy 2 years ago. The patient had been diagnosed with bone metastases 7 months ago and received treatment with dexamethasone and flutamide. Recently, the patient complained of worsening bone pain with rapid rising of prostate-specific antigen from 45 to 189. F-18 sodium fluoride PET/CT bone scan was conducted to evaluate for progression of bone metastases. Review of the PET/CT images demonstrated a “superscan” pattern of bone metastases with extensive sodium fluoride uptake in the axial skeleton, and poor visualization of distal appendicular skeleton and kidneys.
Rheumatic Fever Prophylaxis: A Three-year Study
Authors: T. A. Hassell, K. L. Stuart
Journal: BMJ (1974)

An effective programme of secondary prophylaxis against rheumatic fever has been established in Barbados over the last three years. Relatively unsophisticated patients collaborated well over prolonged periods. Adequate secondary prophylaxis was achieved with monthly injections of benzathine penicillin, with a resultant decrease in the complication rate. A total of 84 admissions over 32 months from a population of some 60,000 children under the age of 12 emphasizes the need for registration and prophylactic treatment of children with rheumatic fever. The low yield in the survey of schoolchildren between the ages of 5 and 11 and the large number of personnel required for the survey suggest that it might be extended to include older age groups. Automatic screening devices should be used when possible so that more may be screened.