tbuckley/cabot-search huggingface dataset:
Thromboembolic risk stratification based on SPAF clinical criteria in patients with paroxysmal atrial fibrillation and flutter: A prospective transesophageal echocardiography study
Authors: Nadia Benyounes, Lydia Djaouti, Charles Smadja, Catherine Albo, Olivier Belliard, Franck Boccara, Ariel A. Cohen
Journal: Journal of the American College of Cardiology (2003)
P04.21 * MOLECULAR DIAGNOSTICS OF MALIGNANT GLIOMAS USING NEXT-GENERATION GENE PANEL SEQUENCING
Authors: A. Veiser, K. Kaulich, S. Stepanow, M. Wolter, K. Kohrer, G. Reifenberger
Journal: Neuro-Oncology (2014)

Gliomas are histologically classified according to the World Health Organization (WHO) classification of central nervous system tumors into distinct tumor types and malignancy grades ranging from WHO grade I (benign) to WHO grade IV (highly malignant). In recent years, characteristic molecular changes were identified in the various glioma types and WHO grades. Common glioma-associated genetic alterations are mutations in IDH1 or IDH2, TP53 and ATRX in diffuse astrocytic gliomas, mutations in IDH1 or IDH2, CIC and TERT in oligodendrogliomas, mutations in TERT, PTEN, TP53 and H3F3A as well as copy number alterations of EGFR and CDKN2A/B, in glioblastomas, and mutations/genetic rearrangements of BRAF in pilocytic astrocytomas and pleomorphic xanthoastrocytomas. The identification of specific patterns of gene mutations may have diagnostic, prognostic and predictive implications for the individual patients and thus can facilitate more accurate stratification of patients to distinct treatment protocols. In this project, we therefore aimed at the improvement of the molecular diagnostics of gliomas by establishment of next-generation sequencing (NGS) of a glioma-tailored gene panel using the Ion Torrent PGM or Proton platform. For this purpose, we assembled a panel of 20 glioma-associated genes with the Ion AmpliSeqTM Desinger Tool. Our customized glioma panel includes coding sequences and hotspot regions of the genes that are most frequently mutated in gliomas. First runs were performed to determine the number of generated reads and to detect known sequence variations in several samples. To assess the specificity and sensitivity of variant detection, we compared the NGS results with conventional sequencing results and qRT-PCR data. Furthermore, the read output and variant detection of DNA libraries created from DNA extracted from frozen tissue samples and formalin-fixed paraffin-embedded tissue samples was compared. To assess the relationship of diagnostic mutation profiles with histological classification, we have screened a larger number of glioma tissue samples, including tumors of different histologies and WHO grades. Collectively, our results indicate that gene panel NGS is a robust, sensitive and specific method for the diagnostic detection of gene mutations in gliomas, which can be applied on routinely processed tumor samples in a timely and cost-saving manner. Meanwhile, we are working on the establishment of a more comprehensive glioma gene panel that will also allow for the analysis of genes less frequently mutated in glioma as well as cancer genes for which specific drugs are available, thus allowing not only for diagnostic but also also for predictive molecular testing of gliomas. Eventually, we aim to establish gene panel NGS for routine application in the molecular diagnostics of gliomas and other brain tumors.
Health System Reform: The Case for a Single-Payer Approach-Reply
Authors: Jim McDermott
Journal: JAMA (1994)

In Reply.  —Since I submitted my article toTHE JOURNAL, developments in  marketplace have underscored  necessity of moving to a single-payer system to preserve  patient-physician relationship. Notably, on March 22, 1994,  Travelers Insurance Company and Metropolitan Life announced their intention to merge their health care operations. Where are  physicians and  patients in this merger? US Health, a large national managed care corporation, is reported to take 28% of its premium dollar for administrative overhead. That is 28 cents of every dollar as  measure of care denied to patients.  The evidence is overwhelming that the market is responding to health care cost pressures and that that response is to consolidate  control of  for-profit insurance companies over  health care delivery system. We are on  verge of becoming  only country in  world to hand over its health care system to
Elevation of Exhaled Ethane Concentration in Asthma
Authors: PAOLO PAREDI, SERGEI A. KHARITONOV, PETER J. BARNES
Journal: American Journal of Respiratory and Critical Care Medicine (2000)

Ethane is a product of lipid peroxidation as a result of oxidative stress and can be detected in the exhaled air. Oxidative stress plays a role in the pathogenesis of asthma. We measured exhaled ethane in 26 asthmatic subjects (mean age ± SEM, 38 ± 8 yr; 15 male, FEV1 60 ± 4%) and compared it with exhaled nitric oxide (NO) measured by chemiluminescence, a noninvasive marker of oxidative stress and inflammation. Exhaled ethane was collected during a flow- and pressure-controlled exhalation into a reservoir discarding dead space air contaminated with ambient air. A sample of the expired air was analyzed by chromatography. Exhaled ethane levels were elevated in asthma patients not receiving steroid (n = 12, 2.06 ± 0.30 ppb) compared with steroid-treated patients (n = 14, 0.79 ± 0.10 ppb, p < 0.01) and to 14 nonsmoking control subjects (0.88 ± 0.09 ppb, p < 0.05). In patients not receiving steroid treatment there was a positive correlation between exhaled ethane and NO (r = 0.55, p < 0.05) and air trapping assessed by the ratio of residual volume to total lung capacity (RV/ TLC) (r = 0.60, p < 0.05). In addition, untreated patients with FEV1 < 60% predicted value had higher concentrations of ethane (2.86 ± 0.37 ppb) compared with less obstructed patients (FEV1 > 60%, 1.26 ± 0.12 ppb, p < 0.05). NO concentrations were higher in patients not on steroid treatment (14.7 ± 1.7 ppb) than in steroid-treated patients (8.6 ± 0.5 ppb, p < 0.05). Exhaled ethane is elevated in asthma, reduced in steroid-treated patients, and correlates with NO and airway obstruction. It may be a useful noninvasive marker of oxidative stress.
Physicians and Patient Spirituality
Authors: Susan B. LeGrand
Journal: Annals of Internal Medicine (2002)

Letters3 September 2002Physicians and Patient SpiritualitySusan B. LeGrand, MDSusan B. LeGrand, MDCleveland Clinic Foundation; Cleveland, OH 44122Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-137-5_Part_1-200209030-00028 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Dr. Graner's comments in his letter to the editor reflect a misunderstanding of the concept of separation of church and state (not “politics”) (1). The U.S. Constitution requires that the government not sanction a particular religion. It makes no statements about what I may have in my personal, nongovernmental office. My office is my personal space, which contains personal photographs, mementos of travels, gifts from patients, and other reflections of my personality that include symbols of my personal faith. I may choose to wear a cross and thereby proclaim to all my personal beliefs. This in no ...References1. Graner J. Physicians and patient spirituality [Letter]. Ann Intern Med. 2001;135:220. LinkGoogle Scholar2. Bodey GP. Physicians and patient spirituality [Letter]. Ann Intern Med. 2001;135:220. [PMID: 11487495] LinkGoogle Scholar Author, Article, and Disclosure InformationAuthors: Susan B. LeGrand, MDAffiliations: Cleveland Clinic Foundation; Cleveland, OH 44122 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoPhysicians and Patient Spirituality Gerald P. Bodey Physicians and Patient Spirituality John Graner Physicians and Patient Spirituality John Graner Physicians and Patient Spirituality Fritz Baumgartner Physicians and Patient Spirituality John Graner Metrics 3 September 2002Volume 137, Issue 5_Part_1 Page: 372 ePublished: 3 September 2002 Issue Published: 3 September 2002 Copyright & PermissionsCopyright © 2002 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Cloning, expression, and characterization of the human eosinophil eotaxin receptor.
Authors: B L Daugherty, S J Siciliano, J A DeMartino, L Malkowitz, A Sirotina, M S Springer
Journal: The Journal of Experimental Medicine (1996)

Although there is a mounting body of evidence that eosinophils are recruited to sites of allergic inflammation by a number of beta-chemokines, particularly eotaxin and RANTES, the receptor that mediates these actions has not been identified. We have now cloned a G protein-coupled receptor, CC CKR3, from human eosinophils which, when stably expressed in AML14.3D10 cells bound eotaxin, MCP-3 and RANTES with Kds of 0.1, 2.7 and 3.1 nM, respectively. CC CKR3 also bound MCP-1 with lower affinity, but did not bind MIP-1 alpha or MIP-1 beta. Eotaxin, RANTES, and to a lessor extent MCP-3, but not the other chemokines, activated CC CKR3 as determined by their ability to stimulate a Ca(2+) -flux. Competition binding studies on primary eosinophils gave binding affinities for the different chemokines which were indistinguishable from those measured with CC CKR3. Since CC CKR3 is prominently expressed in eosinophils we conclude that CC CKR3 is the eosinophil eotaxin receptor. Eosinophils also express a much lower level of a second chemokine receptor, CC CKR1, which appears to be responsible for the effects of MIP-1 alpha.
Danger of Megavitamin Therapy
Authors: Walter M. Kirkendall, Jeremy J. Hammond
Journal: JAMA (1978)

<h3>To the Editor.—</h3> A 48-year-old black man has been observed in our hypertension clinic for three years. He has severe essential hypertension (blood pressures up to 260/150 mm Hg without treatment) that has been present for at least five years. Treatment has been difficult because of the patient's poor compliance with medication and the severity of his hypertension. In 1972 he suffered a stroke with right-sided hemiplegia; in 1976, an anterior myocardial infarction; and in 1977, a second stroke with right-sided signs. (Each of these catastrophic events occurred when he stopped his medication against medical advice.) His serum creatinine level is 1.3 mg/dl. He has made a satisfactory recovery each time. Following an intensive education program, his compliance and attendance at the clinic has been satisfactory for the past six months. His blood pressure was 160/100 mm Hg when taking 80 mg daily of furosemide (Lasix), 120 mg daily of
Lesion characteristics affect thrombolysis in myocardial infarction flow in acute myocardial infarction: An intravascular ultrasound study
Authors: Jun-ichi Kotani, Gary S. Mintz, Marco T. Castagna, Augusto D. Pichard, Lowell F. Satler, Ron Waksman, William O. Suddath, Kenneth M. Kent, Neil J. Weissman
Journal: Journal of the American College of Cardiology (2003)
Immunohistochemistry in lymphoma diagnosis.
Authors: P A Hall
Journal: BMJ (1986)

policy would address itself particularly to the roles of the Department of Health and Social Security, the regional and district health authorities, and the family practitioner com- mittees, and it would regard the inner cities as a special case requiring special solutions.A more flexible approach to virement between the cash limited allocations to the health authorities on the one hand and the open ended expenditures on the family practitioner services on the other would sharpen the debate about respective roles and functions.Whether such a clear strategic lead will be forthcoming is a matter of guesswork.The lessons from the Policy Study Institute study are not encouraging.
Monocyte-Derived Dendritic Cells Perform Hemophagocytosis to Fine-Tune Excessive Immune Responses
Authors: Hideaki Ohyagi, Nobuyuki Onai, Taku Sato, Satoshi Yotsumoto, Jiajia Liu, Hisaya Akiba, Hideo Yagita, Koji Atarashi, Kenya Honda, Axel Roers, Werner Müller, Kazutaka Kurabayashi, Mayuka Hosoi-Amaike, Naoto Takahashi, Makoto Hirokawa, Kouji Matsushima, Kenichi Sawada, Toshiaki Ohteki
Journal: Immunity (2013)
HIV-1 infection–induced apoptotic microparticles inhibit human DCs via CD44
Authors: Davor Frleta, Carolyn E. Ochoa, Holger B. Kramer, Shaukat Ali Khan, Andrea R. Stacey, Persephone Borrow, Benedikt M. Kessler, Barton F. Haynes, Nina Bhardwaj
Journal: Journal of Clinical Investigation (2012)

Acute HIV-1 infection results in dysregulated immunity, which contributes to poor control of viral infection. DCs are key regulators of both adaptive and innate immune responses needed for controlling HIV-1, and we surmised that factors elicited during acute HIV-1 infection might impede DC function. We derived immature DCs from healthy donor peripheral blood monocytes and treated them with plasma from uninfected control donors and donors with acute HIV-1 infections. We found that the plasma from patients with HIV specifically inhibited DC function. This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. Apoptotic microparticles bound to and inhibited DCs through the hyaluronate receptor CD44. These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity.
The use of granulocyte-macrophage colony-stimulating factor to enhance hematologic parameters of patients with cirrhosis and hypersplenism
Authors: Ahmet Gurakar, Stefano Fagiuoli, Judith S. Gavaler, Tarek Hassanein, Nicholas Jabbour, Harlon I. Wright, Steven A. Deal, Ashot Shah, Manuel Brown, Brian I. Carr, Daniel H. Van Thiel
Journal: Journal of Hepatology (1994)
The A. M. A. as Basis of Reciprocity.
Authors: E. A. Cobleigh
Journal: JAMA (1902)

Chattanooga, Tenn., Jan. 21, 1902. <h3>To the Editor:</h3> —I can not just at present go into a detailed consideration of defense of the proposition, but I wish to heartily endorse the suggestion made by Dr. Skelly in the last issue ofThe Journal, that membership in the American Medical Association be made the basis of reciprocity for license to practice between the various state examining boards. With this as a foundation idea it seems to me that it is possible of elaboration so as to practically apply to this vexed question how to bar quacks and impostors, while at the same time recognizing intelligence, experience and probity towards license for practice throughout our common country, with as little of examination as may prove reasonable in compliance with laws as now existent or contemplated. At some future date I may find opportunity for further argument on this point. At present I
Abstract 40: Optimal Cerebral Oxidation Levels for Brain Protection During Resuscitation in Cases of Refractory Cardiac Arrest
Authors: Noritoshi Ito, Shinsuke Nanto, Ken Nagao, Tetuso Hatanaka, Kei Nishiyama, Yasuji Doi, Yuma Kurozumi, Tomoaki Natsukawa, Masaya Morita, Atsushi Kawata, Ayumu Tsuruoka, Hirotaka Sawano, Hiroyuki Shibata, Noriaki Yamada, Ken-ichiro Okada, Yasuyuki Hayashi, Tatsuro Kai, Toru Hayashi
Journal: Circulation (2011)

Background: Some clinical studies suggest that exposure to hyperoxia after resuscitation from cardiac arrest might worsen anoxic brain injury. Objective: The aim of this study was to investigate the association between regional cerebral oxygen saturation (rSO2) levels measured immediately on hospital arrival and neurological outcome at hospital discharge in cardiac arrest on arrival (CAOA) patients after out-of-hospital cardiac arrest (OHCA) Methods: This study was a prospective cohort study. Of 418 OHCA patients, 186 consecutive non-traumatic, CAOA patients were prospectively registered from April 2009 to March 2011. The rSO2 levels were measured with sensors placed on either side of their forehead using a near-infrared spectroscopy device (INVOS, Covidien, US) within 3 minutes after hospital arrival. We monitored rSO2 levels at least for more than one minute and used the lower of the 2 measured rSO2 levels for analysis. Clinical staff performed ordinary post-cardiac-arrest interventions (PCAIs) regardless of rSO2 levels. We divided eligible patients into the four groups by 20%-rSO2 level stratum. The primary endpoint was good neurological outcome (Cerebral Performance Category; =&lt;2) at hospital discharge according to the “Utstein-style” guideline and we compared neurological outcome among the groups. Results: Among 186 CAOA patients in the PCAIs era, 18 patients (9.7%) had good neurological outcome. However, none in the lowest level group (rSO2=&lt;20%, n=109) had good neurological outcome at hospital discharge. Mortality after CAOA decreased with increasing rSO2 levels. However, rate of good neurological survivors hit a ceiling in the highest rSO2 group (rSO2&gt;60%, n=12). Conclusion: This study suggests that one of the main goals of resuscitation might be to maintain rSO2 levels during resuscitation in cases of refractory cardiac arrest, however rSO2 levels might hit a ceiling for brain protection.
82 Further reduction of ribavirin dose in HCV genotype 2/3 patients receiving peginterferon alpha-2a (40KD) (PEGASYS®) plus ribavirin (COPEGUS®): Interim results of a randomised controlled trial
Authors: P. Ferenci, H. Brunner, H. Laferl, U. Bergholz, M. Rosenbeiger, R. Stauber, A. Maieron, G. Fischer, M. Bischof, P. Steindl-Munda
Journal: Journal of Hepatology (2006)
The quantitative detection of total HER2 load by quantum dots and the identification of a new subtype of breast cancer with different 5-year prognosis
Authors: Chuang Chen, He-Shun Xia, Yi-Ping Gong, Jun Peng, Chun-Wei Peng, Ming-Bai Hu, Xiao-Bo Zhu, Dai-Wen Pang, Sheng-Rong Sun, Yan Li
Journal: Biomaterials (2010)
India finalises tobacco control legislation
Authors: G. Mudur
Journal: BMJ (2001)

The Indian cabinet last week approved a long awaited tobacco control bill that will ban advertisements of tobacco products, outlaw smoking in public places, and deliver stronger health warnings to consumers.

The decision to introduce the bill into parliament during its budget session this month is being viewed as a signal that the government is finally moving to carry out longstanding pledges aimed at curbing the use of tobacco.

The legislation will also ban the sale of tobacco products to people under age 18 years and prohibit tobacco companies from sponsoring sports and …
Targeting community mental health services
Authors: N Ghandi, S Holmes, M Lock, N Purandare
Journal: BMJ (1994)

EDITOR, - We agree with the conclusions drawn by Alison S Conway and colleagues1 and would like to comment on several aspects of their results.

A community based mental health service may be in a position to facilitate admissions, even though it has “avoiding admissions” as one of its goals, but we think that rather than quantifying admissions, finer measures of the effectiveness of such a service (as well as appropriateness of bed use) are whether admissions are planned or …
Chromosome 4 localization of a second gene for autosomal dominant polycystic kidney disease
Authors: D.J.M. Peters, L. Spruit, J.J. Saris, D. Ravine, L.A. Sandkuijl, R. Fossdal, J. Boersma, R. van Eijk, S. Nørby, C.D. Constantinou-Deltas, A. Pierides, J.E. Briessenden, R.R. Frants, G.-J.B. van Ommen, M.H. Breuning
Journal: Nature Genetics (1993)
Controlled and Cardiac-Restricted Overexpression of the Arginine Vasopressin V1A Receptor Causes Reversible Left Ventricular Dysfunction Through Gα <sub>q</sub> -Mediated Cell Signaling
Authors: Xue Li, Tung O. Chan, Valerie Myers, Ibrul Chowdhury, Xue-Qian Zhang, Jianliang Song, Jin Zhang, Jocelyn Andrel, Hajime Funakoshi, Jeffrey Robbins, Walter J. Koch, Terry Hyslop, Joseph Y. Cheung, Arthur M. Feldman
Journal: Circulation (2011)

[Arg8]-vasopressin (AVP) activates 3 G-protein-coupled receptors: V1A, V2, and V1B. The AVP-V1A receptor is the primary AVP receptor in the heart; however, its role in cardiac homeostasis is controversial. To better understand AVP-mediated signaling in the heart, we created a transgenic mouse with controlled overexpression of the V1A receptor.The V1A receptor transgene was placed under the control of the tetracycline-regulated, cardiac-specific α-myosin heavy chain promoter (V1A-TG). V1A-TG mice had a normal cardiac function phenotype at 10 weeks of age; however, by 24 weeks of age, tetracycline-transactivating factor/V1A-TG mouse hearts had reduced cardiac function, cardiac hypertrophy, and dilatation of the ventricular cavity. Contractile dysfunction was also observed in isolated adult cardiac myocytes. When V1A receptor transgene was induced to be expressed in adult mice (V1A-TG(Ind)), left ventricular dysfunction and dilatation were also seen, albeit at a later time point. Because the V1A receptor mediates cell signaling through Gα(q) protein, we blocked Gα(q) signaling by crossing tetracycline-transactivating factor/V1A mice with transgenic mice that expressed a small inhibitory peptide against Gα(q). Gα(q) blockade abrogated the development of the heart failure phenotype in tetracycline-transactivating factor/V1A-TG mice. The heart failure phenotype could be reversed by administration of doxycycline.Our results demonstrate a role for V1A-mediated signaling in the development of heart failure and support a role for V1A blockade in the treatment of patients with elevated levels of vasopressin.
Warm-up programmes in sport
Authors: J. H M Brooks, S. J Erith
Journal: BMJ (2008)

<h3>Background</h3> Delay in help seeking for cancer ‘alarm’ symptoms has been identified as a contributor to delayed diagnosis. <h3>Aim</h3> To understand people’s help-seeking decision making for cancer alarm symptoms, without imposing a cancer context. <h3>Design and setting</h3> Community-based, qualitative interview study in the UK, using purposive sampling by sex, socioeconomic status, and prior help seeking, with framework analysis of transcripts. <h3>Method</h3> Interviewees (<i>n</i> = 48) were recruited from a community-based sample (<i>n</i> = 1724) of adults aged ≥50 years who completed a health survey that included a list of symptoms. Cancer was not mentioned. Participants reporting any of 10 cancer alarm symptoms (<i>n</i> = 915) and who had consented to contact (<i>n</i> = 482) formed the potential pool from which people were invited to an interview focusing on their symptom experiences. <h3>Results</h3> Reasons for help seeking included symptom persistence, social influence, awareness/fear of a link with cancer, and ‘just instinct’. Perceiving the symptom as trivial or ‘normal’ was a deterrent, as was stoicism, adopting self-management strategies, and fear of investigations. Negative attitudes to help seeking were common. Participants did not want to be seen as making a fuss, did not want to waste the doctor’s time, and were sometimes not confident that the GP could help. <h3>Conclusion</h3> Decision making about cancer alarm symptoms was complex. Recognition of cancer risk almost always motivated help seeking (more so than the fear of cancer being a deterrent), assisted by recent public-awareness campaigns. As well as symptom persistence motivating help seeking, it could also have the reverse effect. Negative attitudes to help seeking were significant deterrents.
Letter: G.M.C. and Indian qualifications.
Authors: M D Smith
Journal: BMJ (1975)

It would be well to remind those faced with this difficult therapeutic problem that the only proved portal of entry of candida following cardiac surgery is the infected intravenous drip site, through which antibacterial antibiotics are often being ad- ministered, thus providing a further selective pressure.7An essential part of the manage- ment is the removal and if necessary re- placement of these infected intravenous lines, though this alone cannot be relied upon to be curative.Perhaps it is wise to reconsider the twice-weekly flushing of long-standing infusions with dilute solutions of amphotericin, as suggested by Brennan and his colleagues.