tbuckley/cabot-search huggingface dataset:
Biosafety Assessment of Site-directed Transgene Integration in Human Umbilical Cord–lining Cells
Authors: Jaichandran Sivalingam, Shruti Krishnan, Wai Har Ng, Sze Sing Lee, Toan Thang Phan, Oi Lian Kon
Journal: Molecular Therapy (2010)

Biosafety and efficacy considerations that impede clinical application of gene therapy could be addressed by nonviral ex vivo cell therapy, utilizing transgenic cells that have been comprehensively pre-evaluated for genotoxic potential and transgene expression. We evaluated the genotoxic potential of phiC31 bacteriophage integrase-mediated transgene integration in cord-lining epithelial cells (CLECs) readily cultured from the outer membrane of human umbilical cords, by sequencing and mapping integration sites, spectral karyotyping, high-resolution genome copy number, transcriptome, and transgene copy number analyses and in vivo tumorigenicity. Of 44 independent integration events, <5% were exonic and 85% of modified cells had integrated <or=2 transgene(s). Expression of 95.6% of genes was unaltered in modified cells. Only three small regions showed genome copy number changes that did not correlate with altered gene expression or integration sites. Spectral karyotyping revealed rare nonrecurrent occurrence of three different translocations. Integrase-modified cells were not tumorigenic in immunocompromised mice for at least 4 months. Stable integration of a human factor VIII (FVIII) construct conferred durable FVIII secretion in vitro. Xenoimplantation of FVIII-secreting CLECs in immunocompetent hemophilic mice achieved significant phenotypic correction. Pre-evaluated clonal populations of phiC31 integrase-modified CLECs could be useful as bioimplants for monogenic diseases such as hemophilia.
Legionella pneumophila Antibody in Hospital Employees
Authors: ADELE JOSEPHSON
Journal: Annals of Internal Medicine (1979)

Letters1 October 1979Legionella pneumophila Antibody in Hospital EmployeesADELE JOSEPHSON, R.N., B.S.ADELE JOSEPHSON, R.N., B.S.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-91-4-653 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: I read "Prevalence of Antibody to the Legionnaires' Disease Bacterium in Hospital Employees" in the April issue (1) with great interest because the authors' suggestion that person-to-person transmission of Legionnaires' disease bacterium does occur is contrary to popular belief.Another study in the same issue (2) used the same laboratory methods to carry out a seroepidemiologic survey of Michigan residents. The authors presented seroprevalence rates in those in the > 1:16 category because they believed that titers below 1:128 might indicate past infection. Their finding of an overall prevalence of 22.5% was far lower than the total...References1. SARAVOLATZ L, ARKING L, WENTWORTH B, and QUINN E. Prevalence of antibody to the Legionnaires' disease bacterium in hospital employees. Ann Intern Med. 1979:90:601-3. LinkGoogle Scholar2. EDSON D, STIEFEL H, WENTWORTH B, and WILSON D. Prevalence of antibodies to Legionnaires' disease: a seroepidemologic survey of Michigan residents using the hemagglutination test. Ann Intern Med. 1979;90:691-3. LinkGoogle Scholar3. COLTON T. Statistics in Medicine. Boston: Little, Brown & Company; 1974:174. Google Scholar1. SARAVOLATZ L, ARKING L, WENTWORTH B, and QUINN E. Prevalence of antibody to the Legionnaires' disease bacterium in hospital employees. Ann Intern Med. 1979;90:601-3. LinkGoogle Scholar2. EDSON D, STIEFEL H, WENTWORTH B, and WILSON D. Prevalence of antibodies to Legionnaires' disease: a seroepidemiologic survey of Michigan residents using the hemagglutination test. Ann Intern Med. 1979;90:691-3. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: ADELE JOSEPHSON, R.N., B.S.Affiliations: State University Hospital Brooklyn, NY 11203 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 October 1979Volume 91, Issue 4Page: 653-654KeywordsLegionella pneumophilaLegionellosisResearch laboratories ePublished: 1 December 2008 Issue Published: 1 October 1979 PDF downloadLoading ...
Review: Combinations of clinical findings had moderate sensitivity and specificity for diagnosing the irritable bowel syndrome
Authors: Ford Bursey
Journal: Annals of Internal Medicine (2009)

Source Citation Ford AC, Talley NJ, Veldhuyzen van Zanten SJ, et al. Will the history and physical examination help establish that irritable bowel syndrome is causing this patient’s lower gastrointestinal tract symptoms? JAMA. 2008;300:1793-805. 18854541
IgG4-Related Skin Disease
Authors: Julia S. Lehman, Mark R. Pittelkow, Thomas C. Smyrk
Journal: JAMA Dermatology (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Dermatology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Dermatology journal
Risk of connective tissue disease and related disorders among women with breast implants: a nation-wide retrospective cohort study in Sweden
Authors: O. Nyren, L. Yin, S. Josefsson, J. K McLaughlin, W. J Blot, M. Engqvist, L. Hakelius, J. D B. Jr, H.-O. Adami
Journal: BMJ (1998)

<h3>Abstract</h3> <b>Objective:</b> To examine the relation between connective tissue disease and related conditions and breast implants. <b>Design:</b> Retrospective cohort study of all women in the Swedish national inpatient registry who underwent breast augmentation surgery with artificial implants during 1964-93, compared with women who underwent breast reduction surgery during the same period. <b>Setting:</b> Sweden. <b>Subjects:</b> 7442 women with implants for cosmetic reasons or for reconstruction after breast cancer surgery and 3353 women with breast reduction surgery. <b>Main outcome measures:</b> Subsequent hospitalisation for definite connective tissue diseases (rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis, dermatomyositis, and Sjögren9s syndrome) or related disorders. <b>Results:</b> 29 women with implants were hospitalised for definite connective tissue disease compared with 25.5 expected based on general population rates (standardised hospitalisation ratio 1.1 (95% confidence interval 0.8 to 1.6)). There were no diagnoses of systemic sclerosis, and no significant excess in risk for polymyalgia rheumatica, fibromyalgia, and several related disorders. Among women who underwent breast reduction surgery, 14 were hospitalised for definite connective tissue disease compared with 10.5 expected (standardised hospitalisation ratio 1.3 (0.7 to 2.2)). Compared with the breast reduction group, women with breast implants showed a slight reduction for all definite connective tissue disease (relative risk 0.8 (95% confidence interval 0.5 to 1.4)). <b>Conclusions:</b> This large nationwide cohort study shows no evidence of association between breast implants and connective tissue disease. <h3>Key messages</h3> Anecdotal reports have suggested that silicone breast implants may cause immunological disorders In a nationwide study of over 7000 Swedish women with breast implants and a control group of over 3000 women who had undergone breast reduction surgery, we found no excess risk for connective tissue disease Of the women with implants, 29 were hospitalised for connective tissue disease compared with the 25.5 expected from national hospital discharge rates, while, of those who underwent breast reduction, 14 were hospitalised compared with the 10.5 expected Direct comparison of the two groups of women showed that those with implants had a slightly lower risk of connective tissue disease There is little likelihood of an association between breast implants and connective tissue disease
BCR/ABL induces expression of vascular endothelial growth factor and its transcriptional activator, hypoxia inducible factor-1α, through a pathway involving phosphoinositide 3-kinase and the mammalian target of rapamycin
Authors: Matthias Mayerhofer, Peter Valent, Wolfgang R. Sperr, James D. Griffin, Christian Sillaber
Journal: Blood (2002)
Relaxin decreases renal interstitial fibrosis and slows progression of renal disease
Authors: Sandra L. Garber, Yelena Mirochnik, Carolyn S. Brecklin, Elaine N. Unemori, Ashok K. Singh, Leonid Slobodskoy, Beverly H. Grove, Jose A.L. Arruda, George Dunea
Journal: Kidney International (2001)
Carcinoembryonic Antigen Test: Facts and Fantasy
Authors: Charles G. Moertel
Journal: JAMA (1974)

<h3>To the Editor.—</h3> In the February 18 issue,The Journal's medical news(227:731, 1974) announced Food and Drug Administration (FDA) approval of the carcinoembryonic antigen (CEA) test for cancer detection. News service releases have resulted in headlines for the new cancer test in almost every newspaper in the land and around the world. As might have been anticipated, the lay press took dramatic license in interpreting the original announcement. For example, one West German release stated that the CEA test would detect cancer several months before the body gave any other signs. Predictably, demand for this test has been booming among cancerophobes both here and abroad. After federal approval, it might seem presumptuous to question the validity of the claims made for the CEA test; yet, the physician must assume individual responsibility for critical appraisal of any innovation that could have such a great impact on patient care. The FDA-approved
Amino acid substitution at peptide-binding pockets of HLA class I molecules increases risk of severe acute GVHD and mortality
Authors: Joseph Pidala, Tao Wang, Michael Haagenson, Stephen R. Spellman, Medhat Askar, Minoo Battiwalla, Lee Ann Baxter-Lowe, Menachem Bitan, Marcelo Fernandez-Viña, Manish Gandhi, Ann A. Jakubowski, Martin Maiers, Susana R. Marino, Steven G. E. Marsh, Machteld Oudshoorn, Jeanne Palmer, Vinod K. Prasad, Vijay Reddy, Olle Ringden, Wael Saber, Stella Santarone, Kirk R. Schultz, Michelle Setterholm, Elizabeth Trachtenberg, E. Victoria Turner, Ann E. Woolfrey, Stephanie J. Lee, Claudio Anasetti
Journal: Blood (2013)
Quantitation of Carotid Bruits
Authors: David M. Dawson
Journal: New England Journal of Medicine (1975)

In any one year, at least 4500 Massachusetts residents will suffer an attack of transient cerebral ischemia. By a wide margin, the commonest cause of this event will have been stenosis or intimal ulceration in the large vessels of the neck. Clinically important extracranial vascular disease, as judged by arteriography or autopsy, is observed in 75 to 90 per cent of all patients with transient ischemic attacks or strokes,1 although the exact mechanism leading to stroke is rarely understood. Ideally, it would be desirable if all patients with transient ischemic attacks could be investigated for the presence of stenosis of . . .
Narrative constructions and the life history issue in brain–emotions relations
Authors: Zsolt Unoka, Eszter Berán, Csaba Pléh
Journal: Behavioral and Brain Sciences (2012)

Abstract Emotional reactions are rather flexible, due to the schema-like organization of complex socio-emotional situations. Some data on emotion development, and on certain pathological conditions such as alexithymia, give further support for the psychological constructivist view put forward by Lindquist et al. Narrative organization is a key component of this schematic organization. The self-related nature of narrative organization provides scaffolding to the contextual dependency of emotions.
Therapeutic Synergy of Oral Taxane BMS-275183 and Cetuximab <b> <i>versus</i> </b> Human Tumor Xenografts
Authors: William C. Rose, Robert Wild
Journal: Clinical Cancer Research (2004)

Abstract Purpose: Combination therapy consisting of an oral taxane, BMS-275183, and the anti-epidermal growth factor receptor monoclonal antibody, cetuximab, was assessed for enhanced therapeutic benefit in preclinical tumor models. Experimental Design: Mice bearing human tumor xenografts, either L2987 lung or GEO colon carcinoma, were administered the aforementioned treatments singly or in combination regimens. Delays in tumor growth and tumor-free status were evaluated and combination treatments were assessed relative to optimal solo treatments. Results: Combination therapies with the oral taxane plus cetuximab were tolerated and therapeutic synergistic outcomes obtained. The therapeutic enhancements were &amp;gt;1 log cell kill greater than the antitumor effect caused by either solo agent applied optimally. For example, at the maximum-tolerated dose of BMS-275183, 60 mg/kg/administration, given p.o. once every 3 days for a total of six administrations (q3d×6), 1.0 gross log cell kill was achieved in mice bearing well established (100 to 200 mg) s.c. implanted L2987 tumors. Cetuximab, at an optimal dose of 1 mg/mouse, given i.p. q3d×6, produced 1.3 log cell kill. When cetuximab, 1 mg/mouse, i.p., plus BMS-275183, 25 mg/kg/administration, p.o., were both given q3d×6, the result was 2.6 log cell kill with three of eight mice cured (P &amp;lt; 0.01). Similar efficacy benefits were obtained in the GEO tumor model. Conclusions: The combination of oral taxane BMS-275183 plus cetuximab provided therapeutically synergistic antitumor activity in two different human tumor xenograft models. Clinical evaluation of this combination is recommended.
Induction of early-immediate genes by tumor necrosis factor ? contribute to liver repair following chemical-induced hepatotoxicity
Authors: A. Bruccoleri, R. Gallucci, D. R. Germolec, P. Blackshear, P. Simeonova, R. G. Thurman, M. I. Luster
Journal: Hepatology (1997)

We and others have shown that tumor necrosis factor alpha (TNF-alpha) expression is increased in the livers of experimental animals following exposure to the chemical hepatotoxin, carbon tetrachloride (CCl4). Because TNF-alpha is involved in mediating inflammatory responses, its elevated expression is presumed to be associated with potentiating hepatotoxicity and/or aiding in liver repair processes. To study the role of TNF-alpha in chemical-induced hepatotoxicity, mice were administered neutralizing antibodies to TNF-alpha before administration of low, but hepatotoxic, doses of CCl4. Antibody treatment prevented CCl4-mediated increases in early-immediate gene expression associated with liver regeneration, including expression of c-jun and c-fos proto-oncogenes, as well as DNA binding of the activator protein-1 (AP-1) nuclear transcription factor. Hepatocyte proliferation following CCl4 treatment was also reduced in anti-TNF-alpha antibody-treated mice, as evidenced by a lack of proliferating cell nuclear antigen (PCNA) staining. Antibody treatment slightly delayed liver repair processes, as evidenced by extending the period in which plasma liver enzyme levels were increased and hepatocellular necrosis could be observed. Consistent with the above observations, injection of recombinant TNF-alpha into control mice induced rapid expression of c-jun and c-fos proto-oncogenes. Taken together, these results indicate that TNF-alpha positively modulates liver recovery following CCl4 exposure presumably by stimulating early-immediate genes involved in hepatic mitogenesis, a phenomenon also observed following partial hepatectomy.
Ruptured Myocardial Infarct with Survival for Three Weeks
Authors: Peter T. Lampesis, William D. Crandall, Samuel J. King
Journal: New England Journal of Medicine (1953)

IT is apparent from a review of the literature that rupture of the heart is an important consideration in the complications of acute myocardial infarction. Usually, death occurs forthwith. In the case reported below, there was an opportunity during the clinical course to make a diagnosis of hemopericardium from cardiac rupture. So rare is such a condition that when the x-ray silhouette of the heart with an established infarction shows what appears to be a "pericardial effusion" and fails to clear, there is justification for doing a pericardial tap to make a precise diagnosis.Case ReportC. A., a 64-year-old . . .
Inhibition of MMP-9 by a selective gelatinase inhibitor protects neurovasculature from embolic focal cerebral ischemia
Authors: Jiankun Cui, Shanyan Chen, Chunyang Zhang, Fanjun Meng, Wei Wu, Rong Hu, Or Hadass, Tareq Lehmidi, Gregory J Blair, Mijoon Lee, Mayland Chang, Shahriar Mobashery, Grace Y Sun, Zezong Gu
Journal: Molecular Neurodegeneration (2012)

Abstract Background Cerebral ischemia has been shown to induce activation of matrix metalloproteinases (MMPs), particularly MMP-9, which is associated with impairment of the neurovasculature, resulting in blood–brain barrier breakdown, hemorrhage and neurodegeneration. We previously reported that the thiirane inhibitor SB-3CT, which is selective for gelatinases (MMP-2 and −9), could antagonize neuronal apoptosis after transient focal cerebral ischemia. Results Here, we used a fibrin-rich clot to occlude the middle cerebral artery (MCA) and assessed the effects of SB-3CT on the neurovasculature. Results show that neurobehavioral deficits and infarct volumes induced by embolic ischemia are comparable to those induced by the filament-occluded transient MCA model. Confocal microscopy indicated embolus-blocked brain microvasculature and neuronal cell death. Post-ischemic SB-3CT treatment attenuated infarct volume, ameliorated neurobehavioral outcomes, and antagonized the increases in levels of proform and activated MMP-9. Embolic ischemia caused degradation of the neurovascular matrix component laminin and tight-junction protein ZO-1, contraction of pericytes, and loss of lectin-positive brain microvessels. Despite the presence of the embolus, SB-3CT mitigated these outcomes and reduced hemorrhagic volumes. Interestingly, SB-3CT treatment for seven days protected against neuronal laminin degradation and protected neurons from ischemic cell death. Conclusion These results demonstrate considerable promise for the thiirane class of selective gelatinase inhibitors as potential therapeutic agents in stroke therapy.
Digging for Philosophical Roots
Authors: Samuel Vaisrub
Journal: JAMA (1979)

In the "Dig at Cnidus" (<i>The New Yorker</i>, July 17, 1978, pp 33-69), Katherine Bouton omits no details in her comprehensive account of the history of this town—comprehensive, that is, except for the major role that it played in the history of medicine. There is no mention of the great School of Cnidus, which vied with that of Cos—the island is referred to twice without a word about Hippocrates—for dominance in the practice and philosophy of medicine. Such ignoration (or is it ignorance?) is not a unique phenomenon. A visitor to Salerno, Italy, would be hard put to find someone who knows anything about the first European school of medicine, which flourished there during the early Middle Ages. Nor is it confined to the laity. Despite a seeming resurgence of interest in the history of medicine and in the humanities in medical schools, a general air of dilettantism prevails. Much
METASTATIC CONJUNCTIVITIS IN GONORRHEA.
Authors: JAMES J. CARROLL
Journal: JAMA (1907)

Although gonorrhea was known to the ancients and ophthalmia neonatorum to the early Greeks and Arabians,<sup>1</sup>the true relation of the parent disease to its affiliated inflammations of the eye was still very imperfectly understood even at the beginning of the eighteenth century. While many of the primitive physicians appear to have fully recognized the ophthalmia of infants, it was not until 1750<sup>2</sup>that it was believed to depend on the vaginal discharge of the mother. Before this the theories as to its origin were rather vague, the old physicians of India, for example, ascribing it to the bad character of the mother's milk. In later years the ophthalmia of adults was thought by some to be due to gonorrheal virus seeking another outlet in consequence of suppression of the urethral discharge. Acting on this view Jüngken<sup>3</sup>recommended, in his text-book, the reestablishment of the flow from
The Lumleian Lectures on the Pathological Relations of the Voice and Speech
Authors: J. S. Bristowe
Journal: BMJ (1879)

<b>Targeting specificity has been an essential issue for applying genome editing systems in functional genomics, precise medicine and plant breeding. Understanding the scope of off-target mutations in Cas9 or Cpf1-edited crops is critical for research and regulation. In plants, only limited studies had used whole-genome sequencing (WGS) to test off-target effects of Cas9. However, the cause of numerous discovered mutations is still controversial. Furthermore, WGS based off-target analysis of Cpf1 has not been reported in any higher organism to date. Here, we conducted a WGS analysis of 34 plants edited by Cas9 and 15 plants edited by Cpf1 in T0 and T1 generations along with 20 diverse control plants in rice, a major food crop with a genome size of ~380 Mb. The sequencing depth ranged from 45X to 105X with reads mapping rate above 96%. Our results clearly show that most mutations in edited plants were created by tissue culture process, which caused ~102 to 148 single nucleotide variations (SNVs) and ~32 to 83 insertions/deletions (indels) per plant. Among 12 Cas9 single guide RNAs (sgRNAs) and 3 Cpf1 CRISPR RNAs (crRNAs) assessed by WGS, only one Cas9 sgRNA resulted in off-target mutations in T0 lines at sites predicted by computer programs. Moreover, we cannot find evidence for bona fide off-target mutations due to continued expression of Cas9 or Cpf1 with guide RNAs in T1 generation. Taken together, our comprehensive and rigorous analysis of WGS big data across multiple sample types suggests both Cas9 and Cpf1 nucleases are very specific in generating targeted DNA modifications and off-targeting can be avoided by designing guide RNAs with high specificity</b>.
Sexually transmitted diseases and anal papillomas.
Authors: J. Sherrard, D. Barlow, J. S. Bingham, R. N. Thin
Journal: BMJ (1992)

taken.'They also, however, noted a rise in the number of cases of homosexually acquired gonorrhoea in 1990, with a rate of rectal infection of 6 9%.In the three genitourinary medicine clinics in Riverside Health Authority 59 episodes of confirmed anorectal gonococcal infection occurred in 57 homosexual or bisexual men attending between 30 March and 8 October this year.Of these 57 men, 17 were known to be HIV seropositive and 19 HIV seronegative and 21 were untested for HIV antibodies.Thus while the rate of detection of rectal gonorrhoea in homosexual and bisexual men attending genitourinary medicine departments in this area is much lower than in the era before AIDS, there are no grounds for complacency as these men, regardless of their HIV status, are presumably continuing to have unprotected anal sex or failure of barrier methods.