tbuckley/cabot-search huggingface dataset:
Abstract 5401: Investigation of sub-tumor accumulation of PRINT nanoparticles reveals dose and route of administration dependence on particle association
Authors: Luke Roode, Tao Bo, Jillian Perry, J. Chris Luft, James E. Bear, Ian J. Davis, Joseph M. DeSimone
Journal: Cancer Research (2014)

Abstract The assessment of nanoparticle accumulation in tumors and other organs uses a whole organ-based approach. These techniques, however, neglect the complex composition of tumors which multiple types of cells other than cancer cells(e.g. endothelial cells, macrophages). For delivery of certain therapeutics, identification of precisely which cells are associating with particles is critical. Using an orthotopic model of melanoma and fluorescently labeled 80x320nm PRINT nanoparticles, we assessed nanoparticle distribution in a cell-type specific manner by flow cytometry. We found that although cancer cells make up 90% of the tumor cells, only a small fraction (1-7%) associate the nanoparticles following intravenous dosing. The fractional association was dependent on the dose used and a plateau is seemingly reached, suggesting a limited number of cells are able to associate with 80x320nm particles. In addition, particle-positive tumor-associated macrophages associated with 4-fold more particles than cancer cells despite constituting approximately 1% of all cells in the tumor. When particles were administered intratumorally, cancer cell association doubles as compared to intravenous dosing. Fluoresence intensity suggests that when dosed intratumorally cancer cells associated with a greater amount of particles, similar to macrophages. Together, these data suggest a biological limitation on the ability of intravenous administration of nanoparticles for nucleic acid delivery and reveal the potential for therapeutic targeting of macrophages. Citation Format: Luke Roode, Tao Bo, Jillian Perry, J. Chris Luft, James E. Bear, Ian J. Davis, Joseph M. DeSimone. Investigation of sub-tumor accumulation of PRINT nanoparticles reveals dose and route of administration dependence on particle association. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5401. doi:10.1158/1538-7445.AM2014-5401
The medical and social fate of the dichromate allergic patient
Authors: Reinhard Breit, Rudolf B.M.Turk
Journal: British Journal of Dermatology (1976)

Journal Article The medical and social fate of the dichromate allergic patient Get access Reinhard Breit, Reinhard Breit Dermatologische und Allergologische Abteilung des Städtischen Krankenhauses 7, München‐ Schwabing, D‐8 München 40, Kölner Platz 1, West Germany Search for other works by this author on: Oxford Academic Google Scholar Rudolf B.M.Türk Rudolf B.M.Türk Dermatologische und Allergologische Abteilung des Städtischen Krankenhauses 7, München‐ Schwabing, D‐8 München 40, Kölner Platz 1, West Germany Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 94, Issue 3, 1 March 1976, Pages 349–351, https://doi.org/10.1111/j.1365-2133.1976.tb04400.x Published: 01 March 1976
Transepithelial migration of neutrophils into the lung requires TREM-1
Authors: Julia Klesney-Tait, Kathy Keck, Xiaopeng Li, Susan Gilfillan, Karel Otero, Sankar Baruah, David K. Meyerholz, Steven M. Varga, Cory J. Knudson, Thomas O. Moninger, Jessica Moreland, Joseph Zabner, Marco Colonna
Journal: Journal of Clinical Investigation (2012)

Acute respiratory infections are responsible for more than 4 million deaths each year. Neutrophils play an essential role in the innate immune response to lung infection. These cells have an armamentarium of pattern recognition molecules and antimicrobial agents that identify and eliminate pathogens. In the setting of infection, neutrophil triggering receptor expressed on myeloid cells 1 (TREM-1) amplifies inflammatory signaling. Here we demonstrate for the first time that TREM-1 also plays an important role in transepithelial migration of neutrophils into the airspace. We developed a TREM-1/3–deficient mouse model of pneumonia and found that absence of TREM-1/3 markedly increased mortality following Pseudomonas aeruginosa challenge. Unexpectedly, TREM-1/3 deficiency resulted in increased local and systemic cytokine production. TREM-1/3–deficient neutrophils demonstrated intact bacterial killing, phagocytosis, and chemotaxis; however, histologic examination of TREM-1/3–deficient lungs revealed decreased neutrophil infiltration of the airways. TREM-1/3–deficient neutrophils effectively migrated across primary endothelial cell monolayers but failed to migrate across primary airway epithelia grown at the air-liquid interface. These data define a new function for TREM-1 in neutrophil migration across airway epithelial cells and suggest that it amplifies inflammation through targeted neutrophil migration into the lung.
Reduction of Serum Cholesterol in Postmenopausal Women With Previous Myocardial Infarction and Cholesterol Malabsorption Induced by Dietary Sitostanol Ester Margarine
Authors: Helena Gylling, Rajaratnam Radhakrishnan, Tatu A. Miettinen
Journal: Circulation (1997)

Background Reduction of serum cholesterol decreases mortality in primary and especially in secondary prevention. We investigated how effectively postmenopausal women with a previous myocardial infarction reduced their serum cholesterol with dietary means by using sitostanol ester rapeseed oil margarine, alone and in combination with statins, and to what extent cholesterol metabolism was affected. Methods and Results The first study group consisted of 22 randomly chosen women with angiographically documented coronary artery disease. Baseline studies on home diet were followed by double-blind, randomized, cross-over studies on margarine without and with sitostanol (3 g/d) ester for 7 weeks in random order. A second group of 10 women on simvastatin consumed sitostanol ester margarine for 12 weeks. Sitostanol ester margarine lowered serum total cholesterol by 13% ( P &lt;.05) and LDL cholesterol by 20% ( P &lt;.01). Sitostanol ester margarine reduced total cholesterol in all patients, LDL cholesterol &lt;2.6 mmol/L (&lt;100 mg/dL) in 32%, and &lt;3.4 mmol/L (&lt;133 mg/dL) in 73% versus none and 27% during the home diet ( P &lt;.01 for both). Combined with simvastatin, sitostanol still reduced total and LDL cholesterol by 11±3% and 16±5% ( P &lt;.01 for both). Sitostanol reduced absorption (−45%), increased fecal elimination (+45% as neutral sterols), and stimulated synthesis (+39%) of cholesterol. High cholestanol and plant sterol (high cholesterol absorption) and low baseline precursor sterol proportions (low cholesterol synthesis) predicted high decreases in serum cholesterol. Conclusions Dietary use of sitostanol ester margarine normalizes LDL cholesterol in about one third of women with previous myocardial infarction, especially in those with high baseline absorption and low synthesis of cholesterol, and in combination with statins reduces the needed drug dose.
Mir-17-5P Promotes Migration of Human Hepatocellular Carcinoma Cells Through the P38 Mitogen-Activated Protein Kinase-Heat Shock Protein 27 Pathway
Authors: Fu Yang, Yixuan Yin, Fang Wang, Yuqi Wang, Ling Zhang, Ying Tang, Shuhan Sun
Journal: Hepatology (2010)

miR-17-5p is overexpressed in hepatocellular carcinoma (HCC), but the specific regulatory mechanisms of miR-17-5p in HCC remain unknown. We investigated the molecular basis of miR-17-5p as an oncogene in human HCC cell lines. Our in vivo and in vitro data indicate that miR-17-5p up-regulates the migration and proliferation of HCC cells. Interestingly, proteomic and western blotting assays revealed that miR-17-5p significantly activates the p38 mitogen-activated protein kinase MAPK pathway and increases the phosphorylation of heat shock protein 27 (HSP27). Our results also suggest that E2F1-dependent down-regulation of Wip1 regulates miR-17-5p-p38-HSP27 signaling. Furthermore, suppression of HSP27 expression by small interfering RNA or the p38 MAPK pathway-specific inhibitor SB203580 decreases the migration of HCC cells overexpressing miR-17-5p but does not reduce their proliferation. Finally, we show that miR-17-5p expression correlates well with HSP27 status in primary human HCC tissues with metastasis. Conclusion: Our findings suggest that the p38 MAPK pathway plays a crucial role in miR-17-5p-induced phosphorylation of HSP27 and, as a consequence, phosphorylated HSP27 enhances the migration of HCC cells. Our data highlight an important role of miR-17-5p in the proliferation and migration of HCC cells and support the potential application of miR-17-5p in HCC therapy. (Hepatology 2010;)
79 INFLUENCE OF AMINOTRANSFERASES LEVEL ON THE CORRELATION OF LIVER STIFFNESS ASSESSED BY ACOUSTIC RADIATION FORCE IMPULSE (ARFI) ELASTOGRAPHY WITH LIVER FIBROSIS-AN INTERNATIONAL MULTICENTER STUDY
Authors: S. Bota, I. Sporea, R. Sirli, H. Tanaka, H. Iijima, R. Badea, M. Lupsor, C. Fierbinteanu-Braticevici, A. Petrisor, H. Saito, H. Ebinuma, M. Friedrich-Rust, C. Sarrazin, H. Takahashi, N. Ono, F. Piscaglia, A. Borghi, M. D'Onofrio, A. Gallotti, M. Peck-Radosavljevic, A. Ferlitsch, A. Popescu, M. Danila
Journal: Journal of Hepatology (2012)
Hepatitis, Bradycardia, and the Use of a Cardiac Pacemaker
Authors: Rica Arnon
Journal: JAMA (1974)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
PSYCHOSOCIAL AND PSYCHOLOGICAL FACTORS RELATED TO DELAYED RECOVERY FROM CONCUSSION IN HIGH SCHOOL STUDENTS
Authors: M Hutchinson, P Comper, B Csenge, D Richards
Journal: British Journal of Sports Medicine (2014)

Background Most of what we know about concussions has been generated from research findings with adult subjects (i.e., athletes with uncomplicated, first concussions achieve full recovery within 7 to 14 days). Emerging research suggests that younger athletes may take longer to recover from concussion than their older counterparts. However, there is little evidence that quantifies the recovery-from-concussion process in younger athletes. Objective To describe recovery time and the contribution of pre-existing psychosocial and learning disabilities in concussion recovery in a population of high school students. Design Retrospective chart review (January 2012-June 2013) of concussions where there was a documented medical clearance date to return to sport or physical activity. Setting Sport medicine clinic. All concussions included occurred in subjects attending an all-boys' high school. Participants 106 sport-related concussions were identified by chart review. 84 of the charts reviewed had definitive medical clearance dates. Of these, 33 (39%) of the students were identified with at least one of the following pre-existing conditions (PEC): Anxiety, Depression, ADHD, or Learning Disability. Main outcome measurements Days to medical clearance. Results Students were medically cleared to return to play on average 38 days following concussion (range 4–243 days). Signiﬁcant differences were identified between the group of students with PEC and the group without PEC. Specifically, Wilcoxon Rank-Sum tests (due to non-normal distributions) identified that the PEC group had significantly longer recovery times compared to those students with PEC (P=.03). Conclusions High school students with concussions returned to full activity well beyond the currently accepted “normal recovery” period of 7–14 days in adults. In addition, pre-existing psychosocial and/or learning disabilities resulted in significantly longer recovery times. This information is clinically useful in post-concussive recovery assessment and education of patients and the team supporting their recovery.
Aurora kinases in childhood acute leukemia: the promise of aurora B as therapeutic target
Authors: S A Hartsink-Segers, C M Zwaan, C Exalto, M W J Luijendijk, V S Calvert, E F Petricoin, W E Evans, D Reinhardt, V de Haas, M Hedtjärn, B R Hansen, T Koch, H N Caron, R Pieters, M L Den Boer
Journal: Leukemia (2012)

We investigated the effects of targeting the mitotic regulators aurora kinase A and B in pediatric acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Aurora protein expression levels in pediatric ALL and AML patient samples were determined by western blot and reverse phase protein array. Both kinases were overexpressed in ALL and AML patients (P<0.0002), especially in E2A-PBX1-translocated ALL cases (P<0.002), compared with normal bone-marrow mononuclear cells. Aurora kinase expression was silenced in leukemic cell lines using short hairpin RNAs and locked nucleic acid-based mRNA antagonists. Aurora B knockdown resulted in proliferation arrest and apoptosis, whereas aurora A knockdown caused no or only minor growth delay. Most tested cell lines were highly sensitive to the AURKB-selective inhibitor barasertib–hydroxyquinazoline–pyrazol–anilide (AZD1152-HQPA) in the nanomolar range, as tested with an MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. But most importantly, primary ALL cells with a high aurora B protein expression, especially E2A-PBX1-positive cases, were sensitive as well. In adult AML early clinical trials, clear responses are observed with barasertib. Here we show that inhibition of aurora B, more than aurora A, has an antiproliferative and pro-apoptotic effect on acute leukemia cells, indicating that particularly targeting aurora B may offer a new strategy to treat pediatric ALL and AML.
Rapid Selection of Group A Recipients among Mass Casualties
Authors: P. D. Stewart
Journal: BMJ (1961)

A simple rapid way of typing potential recipients of group A blood is described. It would be useful in that the supply of blood would be almost doubled for transfusing mass casualties in the event of a thermonuclear war or other disaster. In addition to group O blood (47% of the population is group O), it would make available the large supply of group A blood (42% of the population) for transfusion in A and AB recipients. The test, which consists of adding a drop of blood and a drop of tap water to a slide coated with potent anti-A serum and watching for microscopic agglutination, was conducted on 278 persons under simulated emergency conditions. The results of the tests, carried out by persons with no laboratory experience and poor illumination, showed the possibility of serious errors in matching in only 0.3%. It was concluded that the risk would be justifiable under certain disaster conditions.
Epithelial Phenotype Confers Resistance of Ovarian Cancer Cells to Oncolytic Adenoviruses
Authors: Robert Strauss, Pavel Sova, Ying Liu, Zong Yi Li, Sebastian Tuve, David Pritchard, Paul Brinkkoetter, Thomas Möller, Oliver Wildner, Sari Pesonen, Akseli Hemminki, Nicole Urban, Charles Drescher, André Lieber
Journal: Cancer Research (2009)

We studied the susceptibility of primary ovarian cancer cells to oncolytic adenoviruses. Using gene expression profiling of cancer cells either resistant or susceptible to viral oncolysis, we discovered that the epithelial phenotype of ovarian cancer represents a barrier to infection by commonly used oncolytic adenoviruses targeted to coxsackie-adenovirus receptor or CD46. Specifically, we found that these adenovirus receptors were trapped in tight junctions and not accessible for virus binding. Accessibility to viral receptors was critically linked to depolarization and the loss of tight and adherens junctions, both hallmarks of epithelial-to-mesenchymal transition (EMT). We showed that specific, thus far little-explored adenovirus serotypes (Ad3, Ad7, Ad11, and Ad14) that use receptor(s) other than coxsackie-adenovirus receptor and CD46 were able to trigger EMT in epithelial ovarian cancer cells and cause efficient oncolysis. Our studies on ovarian cancer cultures and xenografts also revealed several interesting cancer cell biology features. Tumors in situ as well as tumor xenografts in mice mostly contained epithelial cells and cells that were in a hybrid stage where they expressed both epithelial and mesenchymal markers (epithelial/mesenchymal cells). These epithelial/mesenchymal cells are the only xenograft-derived cells that can be cultured and with passaging undergo EMT and differentiate into mesenchymal cells. Our study provides a venue for improved virotherapy of cancer as well as new insights into cancer cell biology.
Inhibition of Endothelial Activation: A New Way to Treat Cerebral Malaria?
Authors: Samuel C Wassmer, George J Cianciolo, Valéry Combes, Georges E Grau
Journal: PLoS Medicine (2005)

Background Malaria is still a major public health problem, partly because the pathogenesis of its major complication, cerebral malaria (CM), remains incompletely understood. However tumor necrosis factor (TNF) is thought to play a key role in the development of this neurological syndrome, as well as lymphotoxin α (LT). Methods and Findings Using an in vitro model of CM based on human brain–derived endothelial cells (HBEC-5i), we demonstrate the anti-inflammatory effect of LMP-420, a 2-NH2-6-Cl-9-[(5-dihydroxyboryl)-pentyl] purine that is a transcriptional inhibitor of TNF. When added before or concomitantly to TNF, LMP-420 inhibits endothelial cell (EC) activation, i.e., the up-regulation of both ICAM-1 and VCAM-1 on HBEC-5i surfaces. Subsequently, LMP-420 abolishes the cytoadherence of ICAM-1-specific Plasmodium falciparum–parasitized red blood cells on these EC. Identical but weaker effects are observed when LMP-420 is added with LT. LMP-420 also causes a dramatic reduction of HBEC-5i vesiculation induced by TNF or LT stimulation, as assessed by microparticle release. Conclusion These data provide evidence for a strong in vitro anti-inflammatory effect of LMP-420 and suggest that targeting host cell pathogenic mechanisms might provide a new therapeutic approach to improving the outcome of CM patients.
Effect of oral mexiletine on the cough response to capsaicin and tartaric acid
Authors: M. Fujimura
Journal: Thorax (2000)

<h3>BACKGROUND</h3> The effect of the orally active local anaesthetic mexiletine on the cough response to two different tussive agents, a C-fibre ending stimulator capsaicin and a chemostimulant tartaric acid, was examined in normal subjects. <h3>METHODS</h3> The cough threshold, defined as the lowest concentration of capsaicin (C<sub>5</sub>-CP) or tartaric acid (C<sub>5</sub>-TA) causing five or more coughs, and histamine induced bronchoconstriction were measured three hours after a single oral dose of 300 mg mexiletine or placebo in 14 normal subjects. <h3>RESULTS</h3> Mexiletene in a mean (SE) serum concentration of 0.99 (0.04) μg/ml significantly increased C<sub>5</sub>-TA from a geometric mean (SE) of 32.0 (1.27) mg/ml with placebo to 49.9 (1.34) mg/ml, but C<sub>5</sub>-CP did not differ significantly between treatment with mexiletine (12.2 (1.33) μM) and placebo (14.9 (1.23) μM). <h3>CONCLUSIONS</h3> These results suggest that the cough response to capsaicin and tartaric acid may be mediated in part via different neural pathways.
The Use of Central Venous Oxygen Saturation Measurements in a Coronary Care Unit
Authors: ROBERT H. GOLDMAN
Journal: Annals of Internal Medicine (1968)

A technique for detecting changes in myocardial function in patients with myocardial infarction by serial measurements of central venous oxygen saturation is described. The results obtained in 27 patients are reported. Three cases representing uncomplicated myocardial infarction, myocardial infarction with congestive heart failure, and myocardial infarction with congestive heart failure and shock are presented. The theoretical basis for using central venous oxygen saturation as a guide to myocardial function is discussed.
Stroke Following Chiropractic Manipulation of the Spine
Authors: Robert G. Miller
Journal: JAMA (1974)

STROKE has been previously described as a complication of chiropractic manipulation of the head and neck. The reported cases are notable in two respects. First, the affected patients are predominantly young (under 40 years of age) and have no demonstrable coexisting vascular disease. Second, premonitory symptoms of vertebrobasilar ischemia during neck manipulation were simply not recognized as an absolute contraindication to further mechanotherapy. We wish to reemphasize this avoidable syndrome. <h3>Report of Cases</h3><h3>Case 1.—</h3> A 52-year-old woman complained of weakness on the left side of 24 hours' duration. One month earlier she had begun a series of chiropractic manipulations because of recurrent neck pain that had started after minor injury ten years before. During several of these treatments, she experienced transient vertigo associated with nausea, blurred vision, and scintillating scotomata without loss of vision or headache. Near the end of her last treatment, following a painful rotation of the
Pediatric Diagnosis: Interpretation of Symptoms &amp;amp; Signs in Infants, Children, and Adolescents
Authors: Basil J. Zitelli
Journal: JAMA (1992)

Morris Green's fifth edition of<i>Pediatric Diagnosis</i>remains an excellent compendium of differential diagnosis in common pediatric disorders. The structure of the text varies little from the previous edition with two main sections on the physical examination, followed by a long section of signs and symptoms. An excellent chapter on general principles of the physical examination of children begins the book. It is replete with advice, tricks, and tidbits, from an experienced pediatrician and clinician on how to gain the most information from the examination. It includes such pearls as avoiding eye contact with an infant or child who is about to cry, examining a frightened child in the parent's lap, and reassuring a nervous teenager as one goes along that various findings during the examination are normal. This chapter is a superb lead-off for anyone examining children. Complementing the first chapter like a matched bookend, the last chapter is