tbuckley/cabot-search huggingface dataset:
Gene Transfer Efficiency and Genome-Wide Integration Profiling of Sleeping Beauty, Tol2, and PiggyBac Transposons in Human Primary T Cells
Authors: Xin Huang, Hongfeng Guo, Syam Tammana, Yong-Chul Jung, Emil Mellgren, Preetinder Bassi, Qing Cao, Zheng Jin Tu, Yeong C Kim, Stephen C Ekker, Xiaolin Wu, San Ming Wang, Xianzheng Zhou
Journal: Molecular Therapy (2010)

In this study, we compared the genomic integration efficiencies and transposition site preferences of Sleeping Beauty (SB or SB11), Tol2, and piggyBac (PB) transposon systems in primary T cells derived from peripheral blood lymphocytes (PBL) and umbilical cord blood (UCB). We found that PB demonstrated the highest efficiency of stable gene transfer in PBL-derived T cells, whereas SB11 and Tol2 mediated intermediate and lowest efficiencies, respectively. Southern hybridization analysis demonstrated that PB generated the highest number of integrants when compared to SB and Tol2 in both PBL and UCB T cells. Tol2 and PB appeared more likely to promote clonal expansion than SB, which may be in part due to the dysregulated expression of cancer-related genes near the insertion sites. Genome-wide integration analysis demonstrated that SB, Tol2, and PB integrations occurred in all the chromosomes without preference. Additionally, Tol2 and PB integration sites were mainly localized near transcriptional start sites (TSSs), CpG islands and DNaseI hypersensitive sites, whereas SB integrations were randomly distributed. These results suggest that SB may be a preferential choice of the delivery vector in T cells due to its random integration site preference and relatively high efficiency, and support continuing development of SB-mediated T-cell phase I trials. In this study, we compared the genomic integration efficiencies and transposition site preferences of Sleeping Beauty (SB or SB11), Tol2, and piggyBac (PB) transposon systems in primary T cells derived from peripheral blood lymphocytes (PBL) and umbilical cord blood (UCB). We found that PB demonstrated the highest efficiency of stable gene transfer in PBL-derived T cells, whereas SB11 and Tol2 mediated intermediate and lowest efficiencies, respectively. Southern hybridization analysis demonstrated that PB generated the highest number of integrants when compared to SB and Tol2 in both PBL and UCB T cells. Tol2 and PB appeared more likely to promote clonal expansion than SB, which may be in part due to the dysregulated expression of cancer-related genes near the insertion sites. Genome-wide integration analysis demonstrated that SB, Tol2, and PB integrations occurred in all the chromosomes without preference. Additionally, Tol2 and PB integration sites were mainly localized near transcriptional start sites (TSSs), CpG islands and DNaseI hypersensitive sites, whereas SB integrations were randomly distributed. These results suggest that SB may be a preferential choice of the delivery vector in T cells due to its random integration site preference and relatively high efficiency, and support continuing development of SB-mediated T-cell phase I trials.
Patients are not commodities
Authors: Iona Heath
Journal: BMJ (2006)

Referral management centres have been set up in an attempt to control the flow of patients from generalist to specialist services.1 Those proposing and creating such centres seem to view a referral as a simple administrative transaction, whereas those working in primary care know that a successful referral is a much more complex and challenging phenomenon.

Referrals cannot be understood simply in terms of demand. Many patients have to be persuaded to accept referrals and this requires painstaking and careful negotiation within which wide-ranging fears are explored and discussed. The referral process must be able to respond not only to expressed demand but also to unexpressed need.

Referrals occur either because the diagnosis …
Reply to L.C. Panasci
Authors: Judith Hugh, John Hanson, Maggie Cheang, Torsten Nielsen, Charles Perou, Charles Dumontet, John Reed, Maryla Krajewska, Isabelle Treilleux, Matthieu Rupin, Emmanuelle Magherini, John Mackey, Miguel Martin, Charles Vogel
Journal: Journal of Clinical Oncology (2009)
Tomorrow we'll be sober
Authors: A. Clare
Journal: BMJ (1988)

disadvantages that modern  may bring: to the overloading of available time (a finding familiar in the Uves of physicians and businessmen) and, ultimately, to a time famine, in which even leisure becomes scarce. The final chapter draws all these themes together and suggests some ways in which the harmful effects of time on society and the individual may be mitigated, including a reappraisal of the usual approach to death, a reacquaintence with natural seasonal cycles, and an escape from the daily grind (called here a grid) into a less timebound society. With its broad scope the book will appeal to biologists and sociologists alike; and even those ideas with which one group or the other feels uncomfortable deserve serious con? sideration when described and argued so lucidly. I have two personal points of criticism. It is a pity that the American printer, presumably the same one who produced the original : -viti?^'
Effects of Weight Loss vs Aerobic Exercise Training on Risk Factors for Coronary Disease in Healthy, Obese, Middle-aged and Older Men
Authors: Leslie I. Katzel
Journal: JAMA (1995)

<h3>Objective.</h3> —To compare the effects of weight loss vs aerobic exercise training on coronary artery disease risk factors in healthy sedentary, obese, middle-aged and older men. <h3>Design.</h3> —Randomized controlled trial. <h3>Subjects.</h3> —A total of 170 obese (body mass index, 30±1 kg/m<sup>2</sup>[mean±SEM]), middle-aged and older (61 ±1 years) men. <h3>Interventions.</h3> —A 9-month diet-induced weight loss intervention, 9-month aerobic exercise training program, and a weight-maintenance control group. <h3>Main Outcome Measures.</h3> —Change in body composition, maximal aerobic capacity (Vo<sub>2</sub>max), blood pressure, lipoprotein concentrations, and glucose tolerance. <h3>Results.</h3> —Forty-four of 73 men randomized to weight loss completed the intervention and had a 10% mean reduction in weight (-9.5±0.7 kg;<i>P</i>&lt;.001), with no change in Vo<sub>2</sub>max. Forty-nine of 71 men randomized to aerobic exercise completed the intervention, increased their Vo<sub>2</sub>max by a mean of 17% (<i>P</i>&lt;.001), and did not change their weight, whereas the 18 men who completed in the control group had no significant changes in body composition or Vo<sub>2</sub>max. Weight loss decreased fasting glucose concentrations by 2%, insulin by 18%, and glucose and insulin areas during the oral glucose tolerance test (OGTT) by 8% and 26%, respectively (<i>P</i>&lt;.01). By contrast, aerobic exercise did not improve fasting glucose or insulin concentrations or glucose responses during the OGTT but decreased insulin areas by 17% (<i>P</i>&lt;.001). In analysis of variance, the decrement in fasting glucose and insulin levels and glucose areas with intervention differed between weight loss and aerobic exercise when compared with the control group (<i>P</i>&lt;.05). Similarly, weight loss but not aerobic exercise increased high-density lipoprotein cholesterol levels (+13%) and decreased blood pressure compared with the control group. In multiple regression analyses, the improvement in lipoproteins and glucose metabolism was related primarily to the reduction in obesity. <h3>Conclusions.</h3> —These results suggest that weight loss is the preferred treatment to improve coronary artery disease risk factors in overweight, middle-aged and older men. (<i>JAMA</i>. 1995;274:1915-1921)
Autoimmune Intestinal Pathology Induced by hsp60-Specific CD8 T Cells
Authors: Ulrich Steinhoff, Volker Brinkmann, Uwe Klemm, Peter Aichele, Peter Seiler, Ulrike Brandt, Paul W Bland, Immo Prinz, Ulrich Zügel, Stefan H.E Kaufmann
Journal: Immunity (1999)
Renal Transplantation for Children: Another View
Authors: John B. Reinhart
Journal: JAMA (1988)

Two doctors urge the use of transplant procedures in the treatment of end stage renal disease in children for those children and families whose quality of life is apt to be improved by it but suggest restraint in those whose psychosocial status has demonstrated deterioration. They recommend that parents and physician work together with a child psychiatrist to explain medical problems to children and to translate their responses into the medical system of decision making; the option of no further treatment other than supportive care should be offered to children with extremely poor quality of life.
1069-106 Deaths from heart failure in the Era of the implantable defibrillator
Authors: Jeffrey J Teuteberg, Usha Tedrow, Eldrin F Lewis, Anju Nohria, Sui W Tsang, James C Fang, Michael Givertz, John A Jarcho, Gilbert H Mudge, Kenneth L Baughman, Lynne W Stevenson
Journal: Journal of the American College of Cardiology (2004)
High prevalence of occult hepatitis B in Baltimore injection drug users
Authors: Michael Torbenson, Rajesh Kannangai, Jacquie Astemborski, Steffanie A. Strathdee, David Vlahov, David L. Thomas
Journal: Hepatology (2004)

Occult hepatitis B is defined by the presence of hepatitis B virus (HBV) DNA in a serum or liver in the absence of hepatitis B surface antigen (HBsAg). The prevalence and clinical correlates of occult hepatitis B remain incompletely defined. A cross-sectional study was performed to determine the prevalence of occult hepatitis B in a high-risk cohort composed of 188 injection drug users in Baltimore, Maryland. All individuals had chronic hepatitis C viral infections confirmed by RNA detection and liver biopsy. Serologic assays for HBsAg and core antibody (HBcAb) were performed. Serum HBV DNA was detected using the COBAS HBV AMPLICOR monitor assay (lower limit of detection, 200 HBV copies per milliliter) and a semi-nested polymerase chain reaction (PCR) assay (lower limit of detection, 15 HBV copies per milliliter). Although almost all individuals (96%) were anti-HBC positive, only 8 of 188 (4%) were HBsAg positive. Occult hepatitis B was not identified using the COBAS assay, but was found in 81 of 180 (45%) of individuals using semi-nested PCR. Of the 8 HBsAg positive individuals, HBV DNA was found in 1/8 using the COBAS assay and 6/8 using the nested PCR assay. Overall, liver disease was mild, with a median serum alanine aminotransferase (ALT) of 38 IU/L, median activity grade of 3/18, and median fibrosis stage of 1/6. No association was found between the serum AST (aspartate aminotransferase), activity grade, or stage of liver disease and the presence of occult hepatitis B. Serum ALT levels were slightly higher in patients without occult hepatitis B (46 vs. 35 IU/L), and the median years since first injection drug use was somewhat longer in those without occult hepatitis B (24 vs. 20 years). In conclusion, although further research is needed to assess its clinical significance, there is a high prevalence of occult HBV infection in this cohort of HCV-infected injection drug users. (Hepatology 2004;39:51-57.)
A CASE OF ADIPOSIS DOLOROSA.
Authors: AUGUSTUS A. ESHNER
Journal: JAMA (1898)

In a paper read before the Philadelphia Neurological Society in 1891, Henry<sup>1</sup>reported the case of a married woman, 63 years old, in whom, at the age of 49, somewhat circumscribed, painful, fatty swellings appeared successively in various parts of the body. The patient was an immoderate drinker and had had epileptiform convulsions frequently in infancy. Menstruation had begun at 11 and had ceased abruptly at 35. Sensibility was impaired in proportion to the deposition of fat. The case was described as one of<i>myxedematoid dystrophy</i>, and for the condition the designation<i>paratrophy</i>was proposed. In a paper read before the American Neurological Association in 1888, Dercum<sup>2</sup>reported the case of a widow, 51 years old, in whom the arms began to enlarge at the age of 48 or 49 and were the seat of pain. Back, shoulders, arms and sides of the chest became the seat of
Portrait of Rocco and His Son
Authors: M. Therese Southgate
Journal: JAMA (2006)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Analysis of the role of hepatic PPARγ expression during mouse liver regeneration
Authors: Vered Gazit, Jiansheng Huang, Alexander Weymann, David A. Rudnick
Journal: Hepatology (2012)

Mice subjected to partial hepatectomy (PH) develop hypoglycemia, followed by increased systemic lipolysis and hepatic fat accumulation, prior to onset of hepatocellular proliferation. Strategies that disrupt these metabolic events inhibit regeneration. These observations suggest that alterations in metabolism in response to hepatic insufficiency promote liver regeneration. Hepatic expression of the peroxisome proliferator-activated receptor gamma (PPARγ) influences fat accumulation in the liver. Therefore, the studies reported here were undertaken to assess the effects of disruption of hepatic PPARγ expression on hepatic fat accumulation and hepatocellular proliferation during liver regeneration. The results showed that liver regeneration was not suppressed, but rather modestly augmented in liver-specific PPARγ null mice maintained on a normal diet. These animals also exhibited accelerated hepatic cyclin D1 expression. Because hepatic PPARγ expression is increased in experimental models of fatty liver disease in which liver regeneration is impaired, regeneration in liver-specific PPARγ null mice with chronic hepatic steatosis was also examined. In contrast to the results described above, disruption of hepatic PPARγ expression in mice with diet-induced hepatic steatosis resulted in significant suppression of hepatic regeneration.The metabolic and hepatocellular proliferative responses to PH are modestly augmented in liver-specific PPARγ null mice, thus providing additional support for a metabolic model of liver regeneration. Furthermore, regeneration is significantly impaired in liver-specific PPARγ null mice in the setting of diet-induced chronic steatosis, suggesting that pharmacological strategies to augment hepatic PPARγ activity might improve regeneration of the fatty liver.
Association of Single- vs Dual-Chamber ICDs With Mortality, Readmissions, and Complications Among Patients Receiving an ICD for Primary Prevention
Authors: Pamela N. Peterson, Paul D. Varosy, Paul A. Heidenreich, Yongfei Wang, Thomas A. Dewland, Jeptha P. Curtis, Alan S. Go, Robert T. Greenlee, David J. Magid, Sharon-Lise T. Normand, Frederick A. Masoudi
Journal: JAMA (2013)

<h3>Importance</h3>Randomized trials of implantable cardioverter-defibrillators (ICDs) for primary prevention predominantly used single-chamber devices. In clinical practice, patients often receive dual-chamber ICDs, even without clear indications for pacing. The outcomes of dual- vs single-chamber devices are uncertain.<h3>Objective</h3>To compare outcomes of single- and dual-chamber ICDs for primary prevention of sudden cardiac death.<h3>Design, Setting, and Participants</h3>Retrospective cohort study of admissions in the National Cardiovascular Data Registry's (NCDR) ICD registry from 2006-2009 that could be linked to Centers for Medicare &amp; Medicaid Services fee-for-service Medicare claims data. Patients were included if they received an ICD for primary prevention and did not have a documented indication for pacing.<h3>Main Outcomes and Measures</h3>Adjusted risks of 1-year mortality, all-cause readmission, heart failure readmission, and device-related complications within 90 days were estimated with propensity-score matching based on patient, clinician, and hospital factors.<h3>Results</h3>Among 32 034 patients, 12 246 (38%) received a single-chamber device and 19 788 (62%) received a dual-chamber device. In a propensity-matched cohort, rates of complications were lower for single-chamber devices (3.51% vs 4.72%; P &lt; .001; risk difference, −1.20 [95% CI, −1.72 to −0.69]), but device type was not significantly associated with 1-year mortality (unadjusted rate, 9.85% vs 9.77%; hazard ratio [HR], 0.99 [95% CI, 0.91 to 1.07]; P = .79), 1-year all-cause hospitalization (unadjusted rate, 43.86% vs 44.83%; HR, 1.00 [95% CI, 0.97-1.04]; P = .82), or hospitalization for heart failure (unadjusted rate, 14.73% vs 15.38%; HR, 1.05 [95% CI, 0.99-1.12]; P = .19).<h3>Conclusions and Relevance</h3>Among patients receiving an ICD for primary prevention without indications for pacing, the use of a dual-chamber device compared with a single-chamber device was associated with a higher risk of device-related complications and similar 1-year mortality and hospitalization outcomes. Reasons for preferentially using dual-chamber ICDs in this setting remains unclear.
The Lessons of Success — Revisiting the Medicare Story
Authors: David Blumenthal, James Morone
Journal: New England Journal of Medicine (2008)

After reviewing recently released Oval Office tapes, Blumenthal and Morone conclude that President Lyndon Johnson played a larger role in the passage of Medicare and Medicaid in 1965 than historians have reported. They believe the revised version of events suggests that successful enactment of major health care reform would require strong commitment and speedy action on the part of our next president.
Astrocytoma Mimicking the Features of Pheochromocytoma
Authors: Charles H. Evans, Virginia Westfall, Nuzhet O. Atuk
Journal: New England Journal of Medicine (1972)

ASTROCYTOMA is one of many neoplasms (particularly those of neuroectodermal origin) that may be associated with pheochromocytoma.1 2 3 4 Other intracranial tumors, especially those arising in the posterior fossa, may mimic the clinical symptoms, but not the specific biochemical features of pheochromocytoma.5 Any brain tumor may cause systemic hypertension and mildly increased catecholamine production.6 , 7 Case ReportA 10-year-old boy was admitted to the University of Virginia Hospital on April 5, 1970, with a 4-year history of intermittent nasal speech, mild dysphagia and left-sided frontal headaches. During the year before admission progressively severe episodic emesis, generalized diaphoresis, flushing with a prominent periorbital venous . . .
Segmental Reflex Sympathetic Dystrophy
Authors: Eynat Dotan, Nir Hod, Tifha Horne
Journal: Clinical Nuclear Medicine (2005)

Reflex sympathetic dystrophy (RSD), known also as complex regional pain syndrome, is a well-recognized complex response of the body to trauma, thought to be mediated by the sympathetic nervous system and usually resulting in unrelenting nonanatomic pain, out of proportion to the inciting event. These changes classically affect an entire distal portion of an extremity. A rarer, segmental form of RSD in only a part of an extremity has been described involving discrete anatomic structures. The authors present an uncommon manifestation of RSD involving only the medial aspect of the left hand.
Pathogenesis of Hepatocellular Carcinoma in Hereditary Hemochromatosis: Occurrence in Noncirrhotic Patients
Authors: Michael D. Kew
Journal: Hepatology (1990)

Previous reports have emphasized the association of primary hepatocellular carcinoma in patients with idiopathic hemochromatosis with cirrhosis. In contrast, patients with idiopathic hemochromatosis without cirrhosis have no increased risk of hepatocellular carcinoma. Phlebotomy therapy, by preventing the accumulation of parenchymal iron and subsequent cirrhosis, is believed to prevent hepatocellular carcinoma in the precirrhotic stage of the disease. We report the case of a 67–yr–old man with a 32–yr history of idiopathic hemochromatosis compliacated by cirrhosis, who had reversal of cirrhosis with phlebotomy theray, yet developed hepatocellular carcinoma. There was no serologic or tissue evidence of hepatitis B infection.
CARCINOMA OF THE COLON
Authors: W. H. C. Romanis
Journal: BMJ (1924)

TuxEa I183 2, 19241 I JQUWAL~~~~~~~~~~~~prognosis for pelvic delivery is very good and improves as either diameter increases above the minimum.In my tables o'nly seven cases of this degree are included, in which some trouble occurred; two of these were markedly aellitic, and in four others the foetus was considerably above the average size.Medium Degree.-Themedium degree includes all cases with the conjugate reduced to 9 cm., but not less thani 8 cm., and a transverse reduced below normal but not less than 10.75 cm.These measurements are represented by an external conjugate of 18 cm.but not less than 161 cm., and an intercristal reduced to 25-cm.When the conjuigate is reduced to 9 cm. it precludes the passage of an average biparietal diameter, but the average bitemporal diameter is about 8.25 cm., and capable-of sliglht ireduction.The progniosis in this degree depends upon to what extent the bitemporal can be substituted for the biparietal, and this depends upon the transverse diame'ter.When the transverse is normal, the maximum exchange can occur, the pelvis is -simple flat in type, and the prognosis is good even with the conjugate reduced to 8 cm.On the other hand, with the transverse reduced to 10.75 cmil.nio change from the biparietal can occur, anid its passage depends upon moulding, which has reached its limit of safety at 9 cm.The pelvis' is t.ransversely contracted in type, and the prognosis, altliough good, is not better thlan the simple flat with an 8 cm.conjugate.As both diameters are gradually reduced the prognosis gradually gets worse.Of my cases 157 come into this degree, and 23, or 14.6 per cent., had difficulty greater than low forceps or were premnature.MajorlDegree.-Themajor degree of contraction is when the copjugate is reduced to 8 cm.No imiiprovement is obtained even' with a normal transverse, as the space is not compatible with the average bitemporal diameter.The prognosis is bad for pelvic delivery.Twenty-six cases come into this degree, and 9, or 34.6 per cent., w-ere difficult.In all three degrees of contraction the prognosis is im- proved by a reduction of the foetal head below the normal average, wllile increase in the size of the head makes the prognosis worse.