tbuckley/cabot-search huggingface dataset:
Open ventral hernia repair using ProGrip™ self-gripping mesh
Authors: Steven B. Hopson, Larry E. Miller
Journal: International Journal of Surgery (2015)

Secure mesh fixation in incisional hernia repair is mandatory to prevent mesh dislocation and possible recurrence. Traditional fixation methods have been implicated as a source of chronic postoperative pain. We report 2-year outcomes with a self-gripping mesh for open tension-free repair of large incisional hernia. This prospective case series enrolled 20 patients with large primary incisional hernia (mean defect size: 84 cm2). Patients were electively treated by a single surgeon using a macroporous polyester mesh with resorbable polylactic acid microgrips (ProGrip™, Covidien, Mansfield, MA, USA) using open onlay technique. Main outcomes included pain severity, Carolinas Comfort Scale (CCS), complications, and hernia recurrence. Patients returned for follow-up at 1, 3, 6, 12, and 24 months. Median mesh fixation time was 2 min. Mean operative time was 38 min and blood loss was minimal (50 cc). Most patients (75%) were discharged same day. The only perioperative complication was a minor seroma in one patient. Patient follow-up compliance through 2 years was 100%. Mean pain score was 1.8 at discharge, 0.9 at 1 month, 0.7 at 1 year, and 0 at 2 years. At 2-years, all patients were "very satisfied" with treatment and hernia-specific quality of life was excellent (mean CCS score = 0). No infection, mesh removal, or hernia recurrence occurred during follow-up. Open repair using a self-gripping mesh is a viable treatment option in patients with large incisional hernia. Immediate mesh fixation facilitates a safe and durable tension-free repair.
Primary amyloidosis (AL) in families
Authors: Morie A. Gertz, John P. Garton, Robert A. Kyle
Journal: American Journal of Hematology (1986)

Abstract We report the occurrence of immunoglobulin‐related amyloidosis (AL) in three separate families, each family having two members affected. None of the six patients had evidence to suggest the presence of familial amyloidosis (AF). Previously, immunoglobulin‐related amyloidosis (AL) was considered to be a sporadic disease process. Because of the occasional familial occurrence of other monoclonal gammopathies such as monoclonal gammopathy of undetermined significance, multiple myeloma, and macroglobulinemia of Waldenström, amyloidosis (AL) should be added to the list of immunopathies with a familial predisposition.
Relation between Phase of Menstrual Cycle and Emergency Department Visits for Acute Asthma
Authors: JANICE L. ZIMMERMAN, PRESCOTT G. WOODRUFF, SUNDAY CLARK, CARLOS A. CAMARGO
Journal: American Journal of Respiratory and Critical Care Medicine (2000)

Hormonal fluctuations during the menstrual cycle are hypothesized to influence the course of asthma among women. A recent study found that almost 50% of emergency department (ED) visits occur during the perimenstrual phase. Our prospective cohort study in 64 EDs examined the relation between phase of menstrual cycle and visits for acute asthma. A total of 288 women with acute asthma were evaluated with a standardized patient interview and medical record review after excluding subjects who were pregnant, on hormonal therapy, postmenopausal, status post hysterectomy, had incomplete reproductive data, or whose ED visit fell more than 28 d after their last menstrual period. Only 13% reported reproductive factors as a personal asthma trigger. For all subjects, ED asthma visits were classified by menstrual phase: 33% were preovulatory (Days 5 to 11), 26% were periovulatory (Days 12 to 18), 20% were postovulatory (Days 19 to 25), and 21% were perimenstrual (Days 26 to 4), p = 0.008. There was no significant association between phase of menstrual cycle and asthma severity. Our data indicate that ED visits for acute asthma among women are more frequent during the preovulatory phase in contrast to other studies reporting more visits in the perimenstrual phase.
Spray-applied cell therapy with human allogeneic fibroblasts and keratinocytes for the treatment of chronic venous leg ulcers: a phase 2, multicentre, double-blind, randomised, placebo-controlled trial
Authors: Robert S Kirsner, William A Marston, Robert J Snyder, Tommy D Lee, D Innes Cargill, Herbert B Slade
Journal: The Lancet (2012)
Regulatory T Cells and Toll-Like Receptors in Cancer Therapy
Authors: Rong-Fu Wang
Journal: Cancer Research (2006)

Abstract T regulatory (Treg) cells that suppress immune responses may limit the efficiency of cancer immunotherapy. Recent findings indicate that Toll-like receptors (TLR) directly regulate the suppressive activity of Treg cells. Linking TLR signaling to the functional control of Treg cells may offer new opportunities to improve the outcome of cancer immunotherapy by coadminstration of certain TLR ligands. (Cancer Res 2006; 66(10): 4987-90)
Jejunal Entrapment of a Gastric Balloon
Authors: Arlen Fleisher
Journal: JAMA (1987)

<h3>To the Editor.—</h3> Use of the intragastric balloon for treatment of morbid obesity is a relatively new concept in this country. The balloon is placed into the stomach either blindly or under endoscopic control and then inflated. The exact mechanism of weight loss is unknown, but is believed to be related to early satiation. Although complications exist,<sup>1-5</sup>the need for surgical intervention is unusual and has prompted our following report. <h3>Report of a Case.—</h3> The patient was a 42-year-old, 1.6-m (5 ft 3 in) tall, 95-kg (211-lb) woman who had had a Garren-Edwards gastric balloon (American Edwards Company, Santa Ana, Calif) inserted at another institution for weight control. The balloon was placed under endoscopic control, then filled with approximately 200 mL of air. When fully inflated, the balloon is about the size and shape of a soft drink can. Two weeks following placement, the balloon was changed because it
Clinical Significance of CTNNB1 Mutation and Wnt Pathway Activation in Endometrioid Endometrial Carcinoma
Authors: Yuexin Liu, Lalit Patel, Gordon B. Mills, Karen H. Lu, Anil K. Sood, Li Ding, Raju Kucherlapati, Elaine R. Mardis, Douglas A. Levine, Ilya Shmulevich, Russell R. Broaddus, Wei Zhang
Journal: JNCI Journal of the National Cancer Institute (2014)

Endometrioid endometrial carcinoma (EEC) is the most common form of endometrial carcinoma. The heterogeneous clinical course of EEC is an obstacle to individualized patient care.We performed an integrated analysis on the multiple-dimensional data types including whole-exome and RNA sequencing, RPPA profiling, and clinical data from 271 EEC cases in The Cancer Genome Atlas (TCGA) to identify molecular fingerprints that may account for this clinical heterogeneity. Significance analysis of microarray was used to identify marker genes of each subtype that were subject to pathway analysis. Association of molecular subtypes with clinical features and mutation data was analyzed with the Mann Whitney, Chi-square, Fisher's exact, and Kruskal-Wallis tests. Survival analysis was evaluated with log-rank test. All statistical tests were two-sided.Four transcriptome subtypes with distinct clinicopathologic characteristics and mutation spectra were identified from the TCGA dataset and validated in an independent sample cohort of 184 EEC cases. Cluster II consisted of younger, obese patients with low-grade EEC but diminished survival. CTNNB1 exon 3 mutations were present in 87.0% (47/54) of Cluster II (P < .001) that exhibited a low overall mutation rate; this was statistically significantly associated with Wnt/β-catenin signaling activation (P < .001). High expression levels of CTNNB1 (P = .001), MYC (P = .01), and CCND1 (P = .01) were associated with poorer overall survival in low-grade EEC tumors.CTNNB1 exon 3 mutations are likely a driver that characterize an aggressive subset of low-grade and low-stage EEC occurring in younger women.
Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies.
Authors: C Waltenbaugh
Journal: The Journal of Experimental Medicine (1981)

Using a novel, two-step functional screening procedure, we have isolated hybridoma B cell lines secreting monoclonal antibodies directed against gene products of the I-Jb and I-Jk subregions of the mouse H-2 complex. These monoclonal antibodies act in vitro by allowing nonresponder spleen cells to respond to normally suppressive quantities of poly(Glu50Tyr50) (GT) (WF8 series of anti-I-Jk monoclonal antibodies) or to suboptimal concentration of poly(Glu60Ala30Tyr10) (WF9 series of anti-I-Jb monoclonal antibodies). Some of the culture supernates that show augmenting activity bind GT-specific T cell-derived suppressor factor (GT-TsF), indicating that some monoclonal antiantibodies display a nonspecific enhancing effect, or, more likely, that anti-I-J monoclonal antibodies have been produced against I-J determinants not found on TsF. It is this last possibility that is most intriguing and that might serve as a means for exploring the heterogeneity of the I-J subregion. It is also possible that some of our monoclonal anti-I-J antibodies might detect antigenic determinants selectively expressed on suppressor T cells, helper T cells, and/or macrophages. In addition, we have demonstrated that monoclonal anti-I-J antibodies should be useful in the biochemical characterization and purification of a monoclonal GT-TsF. These haplotype-specific anti-I-J monoclonal antibodies should prove to be powerful tools for future studies exploring the role of I-J gene products in the regulation of specific immune responses.
Ascending Aortic Dilatation Associated With Bicuspid Aortic Valve
Authors: Thomas M. Tadros, Michael D. Klein, Oz M. Shapira
Journal: Circulation (2009)

A scending aortic dilatation occurs more frequently and at a younger age in patients with bicuspid aortic valves (BAV) than it does in patients with normal trileaflet aortic valves (TAV).The clinical significance of the correlation between BAV and dilatation of the ascending aorta is based on 2 factors.2][3][4] Second, aortic dilatation has a propensity for dissection and rupture, making it a potentially lethal disease.Ascending aortic dilatation with BAV warrants frequent monitoring, with possible early prophylactic surgical intervention to prevent dissection or rupture.The purpose of this article is to review the etiology and natural history and to make suggestions regarding management of the disease on the basis of the limited data available.
Response to "Human cytomegalovirus infection in tumor cells of the nervous system is not detectable with standardized pathologico-virological diagnostics"
Authors: C. Cobbs
Journal: Neuro-Oncology (2014)

This manuscript by Baumgarten et al again challenges the existence of human cytomegalovirus (HCMV) infection in glioblastoma (GBM).The findings were first reported by our group in 2002. 1 Before then, there had only been one case report of immunohistochemical (IHC) confirmation of HCMV infection in GBM, and that was in an AIDS patient. 2 Since the 1980's, several groups have shown that CMV could persistently infect fetal human brain, immature glial cells, and GBM cells in culture. 3 -6 After our first report, several groups were unable to confirm our IHC findings. 7,8We believe this was due to the fact that our extensively optimized and sensitive protocols deviated significantly from simpler techniques used for routine CMV immunodetection in AIDS and transplant patient tissues.As a result, when investigators adopted our techniques, their results have been consistent with ours; 9 -11 when they have not used our techniques, their results have usually been negative.Historically, it is not uncommon for new, complex techniques to be controversial and vary widely.This was the case with Her-2 immunostaining in breast cancer in the early years, which was quite controversial and was only accepted when performed on frozen sections until a widely consistent protocol could be developed for formalin-fixed paraffin-embedded (FFPE) sections. 12ur sensitive CMV protocols have been published, and we are in the process of developing an automated IHC and in situ hybridization (ISH) reference lab to perform these studies. 13Critical steps that are involved in these IHC protocols for FFPE GBM specimens include the following:
A systematic review of treatments for guttate psoriasis
Authors: R.J.G. Chalmers, T. O'Sullivan, C.M. Owen, C.E.M. Griffiths
Journal: British Journal of Dermatology (2001)

Background Many different therapies are available for treating guttate psoriasis; however, there appears to be little objective evidence for their efficacy. Objectives This review aims to assess the evidence for the effectiveness of treatments for guttate psoriasis. Antistreptococcal interventions for guttate psoriasis are addressed in a separate review. Methods Studies were identified by searching the Cochrane Clinical Trials Register (Cochrane Library, Issue 3, 1999), Medline® (1966–September 1999), Embase® (1988–September 1999), Salford Database of Psoriasis Trials (to November 1999) and the European Dermato–Epidemiology Network (EDEN) Psoriasis Trials Database (to November 1999) for terms GUTTATE and PSORIASIS. We also searched 100 unselected randomized controlled trials of psoriasis therapy and all 112 randomized controlled trials of phototherapy for psoriasis in the Salford Database of Psoriasis Trials for separate stratification of guttate psoriasis. Results No published report could be found to support or to challenge current commonly used methods of management. Only one trial that met the selection criteria was identified. In this small study of 21 hospitalized patients with guttate psoriasis, intravenous infusion of an n‐3 fatty acid rich lipid emulsion was compared with placebo emulsion containing n‐6 fatty acids. The n‐3 preparation appeared to be of some benefit for patients with guttate psoriasis. Conclusion There is currently no firm evidence on which to base treatment of acute guttate psoriasis. Studies comparing standard treatment modalities, including phototherapy and topical regimens, are required to enable informed decisions on treatment choices to be made.
First 18F-Labeled Tracer Suitable for Routine Clinical Imaging of sst Receptor-Expressing Tumors Using Positron Emission Tomography
Authors: Margret Schottelius, Thorsten Poethko, Michael Herz, Jean-Claude Reubi, Horst Kessler, Markus Schwaiger, Hans-Jürgen Wester
Journal: Clinical Cancer Research (2004)

Abstract Purpose: Despite excellent radionuclide characteristics, no 18F-labeled peptides are available for quantitative peptide receptor mapping using positron emission tomography (PET) so far, mainly due to time-consuming multistep radiosyntheses with limited overall yields. A newly developed two-step chemoselective conjugation method allows rapid and high-yield [18F]fluorination of peptides via oxime formation and was applied for the synthesis of new 18F-labeled carbohydrated Tyr3-octreotate (TOCA) analogs with optimized pharmacokinetics suitable for clinical routine somatostatin-receptor (sst) imaging. Experimental Design: 18F-labeled glucose (Gluc-S-) and cellobiose (Cel-S-) derivatives of aminooxy-functionalized TOCA were synthesized via oxime formation with 4-[18F]fluorobenzaldehyde ([18F]FBOA-peptides). Both the in vitro internalization profile of Gluc-S-Dpr([18F]FBOA)TOCA and Cel-S-Dpr([18F]FBOA)TOCA in hsst2-expressing Chinese hamster ovary cells (dual tracer protocol) and their biodistribution in AR42J tumor-bearing mice were investigated and compared with two [18F]fluoropropionylated ([18F]FP) analogs, Gluc-Lys([18F]FP)TOCA and Gluc-S-Dpr([18F]FP)TOCA. Results: In contrast to [18F]FP-labeling (3 h), chemo-selective [18F]FBOA-formation (50 min) afforded the respective radiopeptides in high yields (65–85%). In vitro, Gluc-S-Dpr([18F]FBOA)TOCA and Cel-S-Dpr([18F]FBOA)-TOCA showed high internalization (139 ± 2 and 163 ± 8 of the reference [125I]Tyr3-octreotide, respectively), which was reflected by high tumor accumulation in vivo [21.8 ± 1.4 and 24.0 ± 2.5% of injected dose/g (1 h), respectively]. How-ever, only Cel-S-Dpr([18F]FBOA)TOCA and Gluc-S-Dpr([18F]FP)TOCA (tumor: 15.1 ± 1.5% of injected dose/g) with its very low accumulation in all of the nontarget organs showed improved tumor:organ ratios compared with Gluc-Lys([18F]FP)TOCA. For Cel-S-Dpr([18F]FBOA)TOCA,tumor:organ ratios (1 h) were 42:1, 27:1, 15:1, 3:1, and 208:1 for blood, liver, intestine, kidney, and muscle, respectively. Conclusion: Due to the fast and high-yield chemoselective radiofluorination strategy and to its excellent pharmacokinetics, Cel-S-Dpr([18F]FBOA)TOCA represents the first tracer suitable for routine clinical application in PET somatostatin receptor imaging.
Bevacizumab induces regression of vestibular schwannomas in patients with neurofibromatosis type 2
Authors: V.-F. Mautner, R. Nguyen, H. Kutta, C. Fuensterer, C. Bokemeyer, C. Hagel, R. E. Friedrich, J. Panse
Journal: Neuro-Oncology (2009)

Bilateral vestibular schwannomas are the hallmark of neurofibromatosis type 2 (NF2), and these tumors impair hearing and frequently lead to deafness. Neurosurgical intervention, the only established treatment, often damages the vestibular nerve. We report 2 cases in which treatment with bevacizumab (for 3 months in one case and 6 months in the other) induced regression of progressive vestibular schwannomas by more than 40% and substantially improved hearing in the patient treated for 6 months. Bevacizumab therapy may thus provide an effective treatment for progressive vestibular schwannomas in patients with NF2.
THE COMPREHENSIVE PSYCHIATRIC UNIT IN THE GENERAL HOSPITAL
Authors: COLIN M. SMITH, D. G. McKERRACHER
Journal: American Journal of Psychiatry (1964)

The effect of admitting an unselected group of 90 committed psychiatric patients to an ordinary, open general hospital psychiatric ward was studied. Comparisons with 90 unselected regular patients were made by means of rating scales of behaviour and psychopathology, and by nursing and medical observations. The results are consistent with the belief that a well-trained staff can now manage practically all psychiatric admissions in a general hospital. The implications are discussed.
Lung Cancer Diagnosis from Proteomic Analysis of Preinvasive Lesions
Authors: S.M. Jamshedur Rahman, Adriana L. Gonzalez, Ming Li, Erin H. Seeley, Lisa J. Zimmerman, Xueqiong J. Zhang, M. Lisa Manier, Sandra J. Olson, Ronak N. Shah, Alison N. Miller, Joe B. Putnam, York E. Miller, Wilbur A. Franklin, William J. Blot, David P. Carbone, Yu Shyr, Richard M. Caprioli, Pierre P. Massion
Journal: Cancer Research (2011)

Abstract Early detection may help improve survival from lung cancer. In this study, our goal was to derive and validate a signature from the proteomic analysis of bronchial lesions that could predict the diagnosis of lung cancer. Using previously published studies of bronchial tissues, we selected a signature of nine matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) mass-to-charge ratio features to build a prediction model diagnostic of lung cancer. The model was based on MALDI MS signal intensity (MALDI score) from bronchial tissue specimens from our 2005 published cohort of 51 patients. The performance of the prediction model in identifying lung cancer was tested in an independent cohort of bronchial specimens from 60 patients. The probability of having lung cancer based on the proteomic analysis of the bronchial specimens was characterized by an area under the receiver operating characteristic curve of 0.77 (95% CI 0.66–0.88) in this validation cohort. Eight of the nine features were identified and validated by Western blotting and immunohistochemistry. These results show that proteomic analysis of endobronchial lesions may facilitate the diagnosis of lung cancer and the monitoring of high-risk individuals for lung cancer in surveillance and chemoprevention trials. Cancer Res; 71(8); 3009–17. ©2011 AACR.
VITAMIN D AND ENDOCHONDRAL OSSIFICATION IN THE RAT AS INDICATED BY THE USE OF SULFUR-35 AND PHOSPHORUS-32
Authors: Dominic D. Dziewiatkowski
Journal: The Journal of Experimental Medicine (1954)

The concentration of inorganic sulfate-sulfur in the serum of vitamin D-deficient rats, 2.6 to 3.5 mg. per cent, was found to be higher than that in the serum of normal rats of the same age, 2.0 mg. per cent. No change was observed following the administration of 25 γ of vitamin D2. In accord with the results of others, it was found that a definitely increased deposition of phosphorus in femurs and tibiae had occurred 36 to 48 hours after the administration of vitamin D2 to vitamin D-deficient rats. An immediate increase in the uptake of sulfate by the skeleton was found using sodium sulfate-S35. As measured by the specific activity of sulfate-sulfur in samples of chondroitin sulfate isolated from the skeletons of the vitamin D-deficient animals and from normal controls receiving equal doses of sulfur-35, the rate of synthesis of chondroitin sulfate in rachitic rats is similar to the rate in normal rats of the same age. Likewise, the incorporation of labelled sulfate into the sulfomuco-polysaccharides of the pelts was found to be equal at 12 hours to that in normal rats. Following the administration of vitamin D2 to deficient animals an increase in the rate of synthesis of the chondroitin sulfate of the skeletons was noted. The radiochemical and radioautographic evidence suggest that there is in vitamin D-deficient rats an impaired utilization of chondroitin sulfate and that vitamin D2 is able to accelerate this process.
Ethanol-induced vasoconstriction causes focal hepatocellular injury in the isolated perfused rat liver
Authors: Masahide Oshita, Nobuhiro Sato, Harumasa Yoshihara, Yoshiyuki Takei, Taizo Hijioka, Hiroyuki Fukui, Moritaka Goto, Takashi Matsunaga, Toru Kashiwagi, Sunao Kawano, Hideyuki Fusamoto, Takenobu Kamada
Journal: Hepatology (1992)

The role of microcirculation in the pathogenesis of alcoholic liver injury was investigated in isolated perfused livers from fed rats. Infusion of ethanol into the portal vein at concentrations ranging from 25 to 200 mmol/L increased portal pressure, which is an indicator of hepatic vasoconstriction, in a concentration-dependent fashion. Portal pressure started to rise immediately on initiation of ethanol load and remained at higher than basal levels throughout the period of ethanol infusion. Release of lactate dehydrogenase, an indicator of cell injury, into the effluent perfusate began to increase after 20 to 30 min of ethanol infusion and continued to increase until the end of the experiment (60 min after the initiation of ethanol infusion). The lactate dehydrogenase level in the effluent perfusate at 60 min was dependent on the ethanol concentration (0 mmol/L, 8 +/- 3 IU/L; 25 mmol/L, 22 +/- 3 IU/L; 50 mmol/L, 51 +/- 11 IU/L; 100 mmol/L, 60 +/- 7 IU/L; 200 mmol/L, 120 +/- 7 IU/L). Simultaneous infusion of sodium nitroprusside (100 mumol/L), a known vasodilator, inhibited significantly the ethanol-induced increases in portal pressure and lactate dehydrogenase release by abolishing hepatic vasoconstriction. In histological examinations focal hepatocellular necrosis, evidenced by trypan blue staining of cell nuclei, was detected predominantly in midzonal and pericentral areas of the liver lobule after 60 min of ethanol infusion. Change in portal pressure during 60 min of ethanol infusion correlated significantly with levels of lactate dehydrogenase after ethanol infusion (r = 0.82; p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
The Tissue Is the Issue: Personalized Medicine for Non-Small Cell Lung Cancer
Authors: Fred R. Hirsch, Murry W. Wynes, David R. Gandara, Paul A. Bunn
Journal: Clinical Cancer Research (2010)

The development of molecular markers is crucial for improving therapy in advanced non-small cell lung cancer (NSCLC). Here, we review perspectives of biomarker-driven personalized therapy in NSCLC, with particular reference to the detection of EML4-ALK gene rearrangements. Different molecular assays, validation, and clinical application to different types of tissue specimens are discussed.