tbuckley/cabot-search huggingface dataset:
Expression of the sarcomeric actin isogenes in the rat heart with development and senescence.
Authors: L Carrier, K R Boheler, C Chassagne, D de la Bastie, C Wisnewsky, E G Lakatta, K Schwartz
Journal: Circulation Research (1992)

Sarcomeric actin genes, alpha-cardiac and alpha-skeletal, are coexpressed in neonatal rodent hearts and are regulated in response to hormonal and hemodynamic stimuli; however, their precise developmental pattern of expression has not been determined, and it is unknown whether they are coexpressed during senescence. We have, therefore, investigated the accumulation of sarcomeric actin transcripts in rat heart during fetal and postnatal development and with senescence by two different techniques: primer extension analysis with an oligonucleotide common to both sarcomeric actins and RNA hybridization with specific cardiac alpha-actin cRNA probes. We found that at 17-19 days in utero both isogenes are coexpressed and alpha-skeletal actin mRNAs represent 28.0 +/- 0.8% of the sarcomeric actin mRNA total. Skeletal actin mRNAs increase to 40% of the total 1 week after birth (NS, p = 0.15), remain constant for 3 weeks, and decrease to less than 20% of the total in ventricles and atria of 1-month-old rats. The alpha-skeletal actin transcripts further decline to less than 5% of the total at 2 months of age and do not reaccumulate in senescent animals. There was no significant difference between male and female rat ventricles. By comparison with the known accumulations of alpha- and beta-myosin heavy chain mRNAs, our results demonstrate that whatever the developmental stage the kinetics of expression for the sarcomeric myosin and actin multigene families are independent.
Acute Hemorrhagic Cystitis
Authors: David S. Folland
Journal: JAMA (1978)

An outbreak of hematuria occurred from May 20 to May 23,1975, among employees of a chemical packaging plant. Nine of 22 workers who packaged the insecticide chlordimeform in a separate shed became severely ill with abdominal pain, dysuria, urgency to void, or hematuria. None of 18 persons who worked in other areas of the plant were affected. Four additional workers who had packaged the chemical during the previous year had a history of similar symptoms. Bladder biopsy specimens from three affected persons showed severe hemorrhagic cystitis; chlordimeform and 2-methyl-4-chloroaniline, a metabolite of chlordimeform, were present in urine specimens collected three days after exposure. The illness lasted from one week to two months; the workers recovered completely. Chlordimeform that was injected subcutaneously into three cats produced similar, though less severe, changes in the bladders of two animals. (<i>JAMA</i>239:1052-1055, 1978)
Closed Sleeve Bandage to Protect Dialysis Shunts
Authors: Willem J. Kolff
Journal: JAMA (1967)

<h3>To the Editor:—</h3> Persons sustained by maintenance hemodialysis must protect their arteriovenous shunts to save their lives. A protective dressing is necessary to avoid trauma, infection, and hemorrhage. The patient must be able to manipulate the bandage, often with one hand only, so that he can carry on routine daily care of the shunt without assistance. A good protective device increases a patient's self-sufficiency, confidence, and safety. We recently have employed the protective device illustrated in the accompanying figures. It is constructed from a single piece of 6inch wide rubberized cotton bandage tapered to fit the patient's arm or leg and fastened with a single strip of 1-inch wide nylon hook and burred fabric closing (Fig 1). The closure allows sufficient leeway so that the bandage may be snugly applied over varying thicknesses of sterile dressings (Fig 2). It also provides proper fit despite the slight stretching of the elastic
Risk factors for wheezing in a subtropical environment
Authors: Ataide A Camara, Jorgete M Silva, Virginia P.L Ferriani, Kátia R.C Tobias, Izolete S Macedo, Márcio A Padovani, Charlotte M Harsi, M.Regina A Cardoso, Martin D Chapman, Eurico Arruda, Thomas A.E Platts-Mills, L.Karla Arruda
Journal: Journal of Allergy and Clinical Immunology (2004)

BackgroundRisk factors for acute wheezing among children in subtropical areas are largely unknown.ObjectiveTo investigate the role of viral infections, allergen sensitization, and exposure to indoor allergens as risk factors for acute wheezing in children 0 to 12 years old.MethodsOne hundred thirty-two children 0 to 12 years of age who sought emergency department care for wheezing and 65 children with no history of wheezing were enrolled in this case-control study. Detection of respiratory syncytial virus antigen, rhinovirus and coronavirus RNA, adenovirus, influenza, and parainfluenza antigens was performed in nasal washes. Total IgE and specific IgE to mites, cockroach, cat, and dog were measured with the CAP system. Major allergens from mites, cockroach, cat, and dog were quantified in dust samples by ELISA. Univariate and multivariate analyses were performed by logistic regression.ResultsIn children under 2 years of age, infection with respiratory viruses and family history of allergy were independently associated with wheezing (odds ratio, 15.5 and 4.2; P = .0001 and P = .008, respectively). Among children 2 to 12 years old, sensitization to inhalant allergens was the major risk factor for wheezing (odds ratio, 2.7; P = .03). High-level allergen exposure, exposure to tobacco smoke, and lack of breast-feeding showed no association with wheezing.ConclusionsSome risk factors for wheezing previously identified in temperate climates were present in a subtropical area, including respiratory syncytial virus infection in infants and allergy in children older than 2 years. Rhinovirus was not associated with wheezing and did not appear to be a trigger for asthma exacerbations.
Overdose Fatality and Surveillance as a Method for Understanding Mortality Trends in Homeless Populations
Authors: Melissa Gambatese, Ann Madsen, Dova Marder
Journal: JAMA Internal Medicine (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
When the Abstract Reports a Well-Tolerated Treatment, the Data Should Support It
Authors: Bruno M. Strebel
Journal: Journal of Clinical Oncology (2013)

Roschewski et al 1 highlight an important issue related to minimal residual disease (MRD) determination and its application in multiple myeloma (MM): the methodologies used to detect MRD will play a critical role in determining or predicting outcome in future.Sensitivity and/or specificity of tests used to determine MRD are impacted by the ease of carrying out the test, the ability to acquire adequate samples for the study, and the availability of technical expertise to perform sensitive assays.Investigators have been using various methods within their technical capabilities.We agree with Roschewski et al 1 that we need a standardized method that defines the number of antibodies used and the timing of testing.Importantly, a critical and often limiting factor is the number of events measured, which will affect our ability to detect MRD with adequate statistical power.Nonetheless, it is gratifying to see that so many institutions are in fact attempting to detect MRD.Another issue is to determine superiority of flow-detected MRD versus molecular MRD using newer sequencing technologies.The molecular MRD method does not depend on the surface expression of molecules, which can change over time and decrease sensitivity.Moreover, one can now evaluate more than 5 million cells and detect one in 10 5 cells.However, molecular MRD deter-mination will require an initial diagnostic sample that contains myeloma cells to identify multiple myeloma-specific immunoglobulin H sequence; newer technologies allow such a determination from even a few thousand cells.Finally, we agree with Roschewski et al that a concerted collaborative clinical study will determine the relative effectiveness of these methods to detect MRD, followed by the development of a consensus methodology to allow for their routine clinical application.
Ascites Due to Chronic Pancreatitis
Authors: Norman M. Jensen
Journal: JAMA (1967)

A patient had massive ascites caused by chronic pancreatitis. Formation of the ascites was accompanied by little pain. The fluid was hemorrhagic and had a high protein content, but could be distinguished from effusions of other etiologies by a very high amylase content. The ascites subsided spontaneously after three weeks.
Financing Long-Term Care: The Need for a National Health Plan
Authors: ARTHUR W. FEINBERG
Journal: Annals of Internal Medicine (1988)

Editorials1 February 1988Financing Long-Term Care: The Need for a National Health PlanARTHUR W. FEINBERG, M.D.ARTHUR W. FEINBERG, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-108-2-295 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAs we approach the end of this century, the United States still has no cohesive and coordinated plan for providing health care to its citizens. Problems of access for the medically indigent, millions of our working people without medical insurance, the untrammeled growth of technology, and the encroachments of for-profit entrepeneurism are some of the issues that demand answers. The solutions remain captive to a free enterprise philosophy that can too easily lose sight of the human considerations involved.Nothing illustrates the dangers of a health care system without a plan better than the rapidly emerging problem of providing long-term...References1. . Financing long-term care. Ann Intern Med. 1988;108:279-88. LinkGoogle Scholar2. SOMERS A. Insurance for long-term care: some definitions, problems, and guidelines for action. N Engl J Med. 1987;317:23-9. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Health Care Financing Subcommittee American College of Physicians North Shore University Hospital Manhasset, New York PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByLong-Term Care: The Need for, and Limits of, an Effective Federal RoleAnne R. Somers 1 February 1988Volume 108, Issue 2Page: 295-296KeywordsHealth careLong-term care Issue Published: 1 February 1988 PDF DownloadLoading ...
Choosing Wisely — The Politics and Economics of Labeling Low-Value Services
Authors: Nancy E. Morden, Carrie H. Colla, Thomas D. Sequist, Meredith B. Rosenthal
Journal: New England Journal of Medicine (2014)

More than 40 medical specialties have identified "Choosing Wisely" lists of five overused or low-value services. But these services vary widely in potential impact on care and spending, and specialty societies often name other specialties' services as low value.
Cyclophosphamide disrupts hepatic sinusoidal endothelium and improves transplanted cell engraftment in rat liver
Authors: Harmeet Malhi, Pallavi annamaneni, Sanjeev Slehria, Brigid Joseph, Kuldeep K. Bhargava, Christopher J. Palestro, Phyllis M. Novikoff, Sanjeev Gupta
Journal: Hepatology (2002)

To determine whether disruption of the hepatic sinusoidal endothelium will facilitate engraftment of transplanted cells, we treated Fischer 344 (F344) rats lacking dipeptidyl peptidase IV (DPPIV) activity with cyclophosphamide (CP). Electron microscopy showed endothelial injury within 6 hours following CP, and, after 24 and 48 hours, the endothelium was disrupted in most hepatic sinusoids. CP did not affect Kupffer cell function. Similarly, CP had no obvious effects on hepatocytes. Intrasplenic transplantation of F344 rat hepatocytes followed by their localization with DPPIV histochemistry showed 3- to 5-fold increases in the number of transplanted cells in CP-treated animals. Transplanted cells integrated in the liver parenchyma more rapidly in CP-treated animals, and hybrid bile canaliculi developed even 1 day after cell transplantation, which was not observed in control animals. To demonstrate whether improved cell engraftment translated into superior liver repopulation, recipient animals were conditioned with retrorsine and two-thirds partial hepatectomy (PH), which induces transplanted cell proliferation. CP treatment of these animals before cell transplantation significantly increased the number and size of transplanted cell foci. In conclusion, disruption of the hepatic sinusoidal endothelium was associated with accelerated entry and integration of transplanted cells in the liver parenchyma. These results provide insights into hepatocyte engraftment in the liver and will help in optimizing liver-directed cell therapy.
Access to Care Is the Centerpiece in the Elimination of Socioeconomic Disparities in Health
Authors: Dennis P. Andrulis
Journal: Annals of Internal Medicine (1998)

Many health care professionals have sustained an almost single-minded conviction that disparities in access to health care across socioeconomic groups are the key reason for the major discrepancies in health status between wealthy persons and poor persons. Others, however, have argued that a host of factors work to create major impediments and that reducing or eliminating financial barriers to health care in particular will do little to reduce discrepancies in health status. This paper, while acknowledging the spectrum of contributing factors, argues that the elimination of financially based differences in access is central to any effort to create equity in outcomes across socioeconomic groups. Through selected review of the many studies on health insurance, access, outcomes, and socioeconomic status, it establishes that a core links affected populations, their difficulty in financing health care, and the threat to their well-being. In so doing, it cites findings that strongly associate lack of insurance (especially for persons who live in poverty), inability to obtain services, and adverse health outcomes. It also uses the example of Medicaid and other coverage for HIV-infected persons in particular as an important positive instance in which leveling the discrepancies in health care across socioeconomic groups can move toward creating quality in access and outcomes. The competitive pressures in today's health care environment threaten to drive socioeconomic groups further apart, especially insured and uninsured persons. However, the recent enactment of state actions, especially the State Child Health Insurance Program, represent powerful examples of health insurance expansion that have lessons for policymakers at all levels for the monitoring and reduction of socioeconomic disparities.
Body Weight, Health, and Longevity
Authors: ARTEMIS P. SIMOPOULOS
Journal: Annals of Internal Medicine (1984)

In the United States, the weight associated with the greatest longevity tends to be below the average weight of the population under consideration, if such weights are not associated with a history of significant medical impairment. Overweight persons tend to die sooner than average-weight persons, especially those who are overweight at younger ages. The effect of being overweight on mortality is delayed and may not be seen in short-term studies. Cigarette smoking is a potential confounder of the relationship between obesity and mortality. Studies on body weight, morbidity, and mortality must be interpreted with careful attention to the definitions of obesity or relative weight used, preexisting morbid conditions, the length of follow-up, and confounders in the analysis. The terminology of body weight standards should be defined more precisely and cited appropriately. An appropriate database relating body weight by sex, age, and possibly frame size to morbidity and mortality should be developed to permit the preparation of reference tables for defining the desirable range of body weight based on morbidity and mortality statistics.
Autologous Hematopoietic Stem-Cell Transplantation for Multiple Myeloma
Authors: Jean-Luc Harousseau, Philippe Moreau
Journal: New England Journal of Medicine (2009)

A 59-year-old man is diagnosed with multiple myeloma. It is recommended that he receive induction therapy followed by high-dose melphalan with autologous hematopoietic stem-cell transplantation. Such transplantation improves the remission rate in myeloma by allowing for the administration of a chemotherapy dose that would otherwise be lethal. This approach is considered appropriate only for relatively young patients without major coexisting illnesses.
Collection of data on ethnic origin in England
Authors: V. S Raleigh
Journal: BMJ (2008)

Is improving, but information needs to be acted on for health inequalities to narrow

A recent BMJ article marking the 60th anniversary of the NHS noted that “the patchy collection of ethnic data by the NHS needs to be improved.”1 Why are such data important, what is the current position, how is it likely to change in the near future, and how does data collection in the UK compare with other countries?

Around 7.7 million people in England (15% of the population) belong to ethnic minority populations, defined as all ethnic groups other than white British (and therefore including white Irish and white other). In some areas—such as the London boroughs of Newham, Tower Hamlets, and Ealing—ethnic minorities comprise more than 50% of the local population. In 46 of the 354 English local authorities, more than 20% of the population is not white British. The population of the United Kingdom is also ethnically diverse and changing, as exemplified by the recent inward migration from Eastern Europe.

Government targets for reducing inequalities in life expectancy focus on “spearhead” areas with the highest mortality and greatest deprivation, but the latest figures show that inequalities are widening.2 In England, about 37% of people from ethnic minority groups live in these areas, and they make up about 20% of the spearhead population. Furthermore, significant epidemiological differences exist between ethnic minority groups, and—contrary to the spirit of personalisation central to the Darzi …
Causes of Neonatal Mortality-Reply
Authors: Max Bader
Journal: JAMA (1984)

<h3>In Reply.—</h3> Dr Raring is correct that<i>JAMA</i>erroneously printed "&lt;500 g" in Table 2 instead of "&lt;1,500 g." However, there is no conflict between the Oregon data and that of SIDS researchers. Infants of low birth weight who survive the postneonatal period are more susceptible to SIDS than infants with higher birth weights. The data in the Tables are only concerned with mortality. Once an infant has survived the postneonatal period, long-term survival is the most probable outcome regardless of birth weight. The Oregon data also show a three to ten times increased risk of SIDS among low-birth-weight infants. The lower the birth weight, the greater the risk. The data provided by Capps et al is an interesting expansion of information on this subject. Unfortunately, the small and heterogeneous nonwhite Oregon population precluded meaningful similar stratification by race and age.
Screening in Chronic Disease
Authors: Madelon Lubin Finkel
Journal: JAMA (1985)

The possibility of identifying individuals in the early stages of a disease and starting treatment when the illness is still reversible is most appealing. Such interaction for chronic conditions as coronary heart disease and cancer is especially attractive because treatment at the later stages of these diseases is often for the alleviation of pain and suffering rather than for cure. Screening programs, originally introduced as a public health measure to detect conditions such as tuberculosis and other diseases that were a hazard to the community, have been established not only to prevent disease but also to detect persons with early, mild, or asymptomatic diseases so that treatment might be instituted sooner rather than later. Screening for cancer of the breast, colon and cervix, in particular, have become part of the routine of a good medical examination. <i>Screening in Chronic Disease</i>is a useful reference source for those interested in the
Dimer-tetramer transition controls RUNX1/ETO leukemogenic activity
Authors: Christian Wichmann, Yvonne Becker, Linping Chen-Wichmann, Vitali Vogel, Anna Vojtkova, Julia Herglotz, Sandra Moore, Joachim Koch, Jörn Lausen, Werner Mäntele, Holger Gohlke, Manuel Grez
Journal: Blood (2010)
A comparative study of eosinophil isolation by different procedures of CD 16‐negative depletion
Authors: J. Chihara, D. Kurachi, T. Yamamoto, H. Yamada, T. Wada, A. Yasukawa, S. Nakajima
Journal: Allergy (1995)

Eosinophils were isolated by the three methods of CD16‐negative depletion: 1) magnetic beads, 2) fluorescence‐activated cell sorter (FACS), and 3) complement reaction. Their purity, yield, and viability were compared. The second procedure produced well purity and viability (94.65 ± 1.51% and 94.98 ± 1.40%, respectively) but low yield of eosinophils (65.47 ± 2.47%). The viability of cells obtained by the third procedure was not efficient (80.83 ± 2.85%), while the purity and the yield were efficient (96.23 ± 1.09% and 90.75 ± 1.72%, respectively). In conclusion, the magnetic beads method (purity: 98.02 ± 0.45%, yield: 91.05 ± 2.43%, viability: 97.57 ± 0.37%) was the most advantageous of these three procedures. Moreover, in the functional assay, radical oxygen products from eosinophils isolated by the procedure with complement reaction were less than with the magnetic beads or FACS procedures.