tbuckley/cabot-search huggingface dataset:
Pyridoxal phosphate as an antisickling agent in vitro.
Authors: J A Kark, P G Tarassoff, R Bongiovanni
Journal: Journal of Clinical Investigation (1983)

Although pyridoxal phosphate is known to inhibit gelation of purified hemoglobin S, antisickling activity has never been demonstrated for intact erythrocytes. We incubated washed erythrocytes at 37 degrees C either in buffer alone, or with added pyridoxal phosphate or pyridoxal, washed these cells, suspended them in untreated buffer, and compared the percent modified hemoglobin, the oxygen affinity, and the extent of sickling under hypoxia. Pyridoxal phosphate modified intracellular hemoglobin more slowly than pyridoxal. Pyridoxal phosphate lowered the oxygen affinity of normal cells, but had no effect on oxygen binding by sickle cells. Pyridoxal increased the oxygen affinity of normal and sickle erythrocytes equally. Pyridoxal phosphate significantly inhibited sickling of sickle or sickle trait erythrocytes (P less than 0.001). Inhibition of sickling by pyridoxal phosphate was largely independent of oxygen binding; whereas inhibition of sickling by pyridoxal was almost entirely dependent on increased oxygen binding. Although pyridoxal phosphate and pyridoxal both inhibit sickling by modification of hemoglobin S, they differ in the kinetics of whole cell modification, the effect on oxygen affinity of intact cells, and the mechanism of action of the antisickling activity.
Elastotic change in the subpapillary and mid-dermal layers in pseudoxanthoma elasticum-like papillary dermal elastolysis
Authors: S. Tajima, Y. Ohnishi, A. Akagi, A. Ishibashi, T. Sasaki
Journal: British Journal of Dermatology (2000)

Journal Article Elastotic change in the subpapillary and mid‐dermal layers in pseudoxanthoma elasticum‐like papillary dermal elastolysis Get access S. Tajima, S. Tajima Department of Dermatology, National Defence Medical College, 3‐2 Namiki, Tokorozawa, Saitama 359‐8513, Japan Search for other works by this author on: Oxford Academic Google Scholar Y. Ohnishi, Y. Ohnishi Department of Dermatology, National Defence Medical College, 3‐2 Namiki, Tokorozawa, Saitama 359‐8513, Japan Search for other works by this author on: Oxford Academic Google Scholar A. Akagi, A. Akagi Department of Dermatology, National Defence Medical College, 3‐2 Namiki, Tokorozawa, Saitama 359‐8513, Japan Search for other works by this author on: Oxford Academic Google Scholar A. Ishibashi, A. Ishibashi Department of Dermatology, National Defence Medical College, 3‐2 Namiki, Tokorozawa, Saitama 359‐8513, Japan Search for other works by this author on: Oxford Academic Google Scholar T. Sasaki T. Sasaki Department of Dermatology, Yokohama City University, School of Medicine, Yokohama, Japan Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 142, Issue 3, 1 March 2000, Pages 586–588, https://doi.org/10.1046/j.1365-2133.2000.03399.x Published: 01 March 2000
End-of-Life Practices in European Intensive Care Units
Authors: E. J. O. Kompanje
Journal: JAMA (2003)

Context Whiletheadoptionofpracticeguidelinesisstandardizingmanyaspectsofpatientcare,ethicaldilemmasareoccurringbecauseofforgoinglife-sustainingtherapiesininten-sive care and are dealt with in diverse ways between different countries and cultures.Objectives To determine the frequency and types of actual end-of-life practices inEuropean intensive care units (ICUs) and to analyze the similarities and differences.Design and Setting A prospective, observational study of European ICUs.Participants Consecutive patients who died or had any limitation of therapy.Intervention Prospectively defined end-of-life practices in 37 ICUs in 17 Europeancountries were studied from January 1, 1999, to June 30, 2000.Main Outcome Measures Comparison and analysis of the frequencies and pat-ternsofend-of-lifecarebygeographicregionsanddifferentpatientsandprofessionals.Results Of31417patientsadmittedtoICUs,4248patients(13.5%)diedorhadalimi-tationoflife-sustainingtherapy.Ofthese,3086patients(72.6%)hadlimitationsoftreat-ments (10% of admissions). Substantial intercountry variability was found in the limita-tionsandthemannerofdying:unsuccessfulcardiopulmonaryresuscitationin20%(range,5%-48%), brain death in 8% (range, 0%-15%), withholding therapy in 38% (range,16%-70%), withdrawing therapy in 33% (range, 5%-69%), and active shortening ofthedyingprocessin2%(range,0%-19%).Shorteningofthedyingprocesswasreportedin7countries.Dosesofopioidsandbenzodiazepinesreportedforshorteningofthedyingprocesswereinthesamerangeasthoseusedforsymptomreliefinpreviousstudies.Limi-tation of therapy vs continuation of life-sustaining therapy was associated with patientage,acuteandchronicdiagnoses,numberofdaysinICU,region,andreligion(
Exercise induced augmentation of cellular and humoral autoimmunity associated with increased cardiac dilatation in experimental autoimmune myocarditis
Authors: J. D HOSENPUD, S. M CAMPBELL, N. R NILES, J. LEE, D. MENDELSON, M. V HART
Journal: Cardiovascular Research (1987)

Journal Article Exercise induced augmentation of cellular and humoral autoimmunity associated with increased cardiac dilatation in experimental autoimmune myocarditis Get access JEFFREY D HOSENPUD, JEFFREY D HOSENPUD ** From the Heart Research Laboratory, Oregon Health Sciences University, Portland, Oregon, USA Address for correspondence and reprints: Dr Jeffrey D Hosenpud, Division of Cardiology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar STEPHEN M CAMPBELL, STEPHEN M CAMPBELL † From the Division of Rheumatology, Oregon Health Sciences University, Portland, Oregon, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar NELSON R NILES, NELSON R NILES ‡ From the Department of Pathology, Oregon Health Sciences University, Portland, Oregon, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar JANE LEE, JANE LEE ** From the Heart Research Laboratory, Oregon Health Sciences University, Portland, Oregon, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar DAVID MENDELSON, DAVID MENDELSON ** From the Heart Research Laboratory, Oregon Health Sciences University, Portland, Oregon, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar MARK V HART MARK V HART ** From the Heart Research Laboratory, Oregon Health Sciences University, Portland, Oregon, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 21, Issue 3, March 1987, Pages 217–222, https://doi.org/10.1093/cvr/21.3.217 Published: 01 March 1987 Article history Received: 15 April 1986 Accepted: 30 July 1986 Published: 01 March 1987
Subunit composition of mink potassium channels
Authors: Ke-Wei Wang, Steve A.N Goldstein
Journal: Neuron (1995)

Expression of minK protein in Xenopus oocytes induces a slowly activating, voltage-dependent, potassium-selective current. Point mutations in minK that alter current gating kinetics, ion selectivity, pharmacology, and response to protein kinase C all support the notion that minK is a structural protein for a channel-type transporter. Yet, minK has just 130 amino acids and a single transmembrane domain. Though larger cloned potassium channels form functional channels through tetrameric subunit association, the subunit composition of minK is unknown. Subunit stoichiometry was determined by coexpression of wild-type minK and a dominant lethal point mutant of minK, which reaches the plasma membrane but passes no current. The results support a model for complete minK potassium channels in which just two minK monomers are present, with other, as yet unidentified, non-minK subunits.
RELATIONSHIP BETWEEN Rh0(D) GENOTYPE AND QUANTITY OF I131 ANTI-Rh0(D) BOUND TO RED CELLS*
Authors: S. P. Masouredis
Journal: Journal of Clinical Investigation (1960)

tive antiglobulin test to this problem, but do not indicate that they were able to determine the Rho(D) genotype with their technique.The availability of a relatively specific 1131 in- complete anti-Rho (D) (7) provided the opportu- nity to study the relationship between the Rho(D) genotype and the Rho(D) antigen content of the red cell.The experimental design adopted for this study was to determine the Rho(D) antigen con- tent of different red cells obtained from the Rho(D) positive donor population entering the Central Blood Bank of Pittsburgh.The frequency
Changing Patterns of Psychiatric Inpatient Care for Children and Adolescents in General Hospitals, 1988–1995
Authors: Kathleen J. Pottick, Donna D. McAlpine, Ross B. Andelman
Journal: American Journal of Psychiatry (2000)

OBJECTIVE: The authors examine patterns in utilization of psychiatric inpatient services by children and adolescents in general hospitals during 1988–1995. METHOD: National Hospital Discharge Survey data were used to describe utilization patterns for children and adolescents with primary psychiatric diagnoses in general hospitals from 1988 to 1995. RESULTS: During the study period, there was a 36% increase in hospital discharges and a 44% decline in mean length of stay, resulting in a 23% decline in the number of bed-days, from more than 3 million to about 2.5 million. The number of nonpsychotic major depressive disorders increased significantly. Discharges from public hospitals have declined, and those from proprietary hospitals have risen. Concurrently, the role of private insurance declined and the role of Medicaid increased. During the period of study, the mean and median length of stay declined most for children and adolescents who were hospitalized in private facilities and those covered by private insurance. Across the United States, the mean length of stay declined significantly; this decline was almost 60% in the West. Discharges also declined in the West, in contrast to the Midwest and the South, where they significantly increased. CONCLUSIONS: Increased numbers of discharges and decreased length of stay may reflect evolving market forces and characteristics of hospitals. Further penetration by managed care into the public insurance system or modifications in existing Medicaid policy could have a profound impact on the availability of inpatient resources.
"THE PATHOGENICITY OF THE FUSIFORM BACILLUS AND SPIRILLUM OF PLAUT VINCENT"
Authors: Henry H. Lichtenberg
Journal: JAMA (1933)

<h3>To the Editor:</h3> —It appears that the Drs. Belding believe our report was not made in good faith. The sixteen (not five) cases of acute ulcerative stomatitis we reported were only of the severe form. None of them went on to a chronic course. We allowed them to run their course untreated because previous reports of cases treated with various therapeutic agents were all lacking in a comparable group of simultaneously observed control cases. An evaluation of a therapeutic procedure in any self-limited disease cannot be made unless this condition is fulfilled. We were therefore interested in observing the natural course of this disease in children and comparing it with the claims made for the various drugs. Since the writing of our communication, there have appeared two short articles by the Drs. Belding which explain more fully what they mean by chronic specific gingivitis (Specific Gingivitis,<i>J. A. Dent. A.</i>
Immunohistochemical detection of pulmonary cytochrome P450IA and metabolic activities associated with P450IA1 and P450IA2 isozymes in lung cancer patients.
Authors: S Anttila, H Vainio, E Hietanen, A M Camus, C Malaveille, G Brun, K Husgafvel-Pursiainen, L Heikkilä, A Karjalainen, H Bartsch
Journal: Environmental Health Perspectives (1992)

The main polycyclic aromatic hydrocarbon-inducible cytochrome P450 was studied in lung tissue from 57 lung cancer patients by immunohistochemistry, using a monoclonal antibody (1-7-1) that recognizes P450IA1 and P450IA2 isozymes. The intensity of immunostaining was compared with the pulmonary activity of a P450IA1-dependent enzyme, aryl hydrocarbon hydroxylase (AHH), and with P450IA2-related metabolic activity estimated from the ratio of caffeine metabolites in urine. Immunostaining was not observed in peripheral lung tissue of nonsmokers or ex-smokers but was seen in the bronchiolar and alveolar epithelium of all patients who were smokers and had a peripheral carcinoma (16/16) and of 60% (10/17) of those who had a bronchial carcinoma. AHH activity was positively related to the intensity of immunostaining, and an almost 2-fold increase due to smoking was detected in the ratios of caffeine metabolites. These results demonstrate that tobacco smoke induces P450IA1 in the lung and probably P450IA2 in the liver, and suggest a role for certain metabolic phenotypes of P450IA1 in peripheral pulmonary carcinoma.
Autologous full-thickness skin substitute for healing chronic wounds
Authors: S. Gibbs, H.M. Van Den Hoogenband, G. Kirtschig, C.D. Richters, S.W. Spiekstra, M. Breetveld, R.J. Scheper, E.M. De Boer
Journal: British Journal of Dermatology (2006)

Background Chronic wounds represent a major problem to our society. Therefore, advanced wound‐healing strategies for the treatment of these wounds are expanding into the field of tissue engineering. Objectives To develop a novel tissue‐engineered, autologous, full‐thickness skin substitute of entirely human origin and to determine its ability to heal chronic wounds. Methods Skin substitutes (fully differentiated epidermis on fibroblast‐populated human dermis) were constructed from 3‐mm punch biopsies isolated from patients to be treated. Acellular allodermis was used as a dermal matrix. After a prior 5‐day vacuum‐assisted closure therapy to prepare the wound bed, skin substitutes were applied in a simple one‐step surgical procedure to 19 long‐standing recalcitrant leg ulcers (14 patients; ulcer duration 0·5–50 years). Results The success rate in culturing biopsies was 97%. The skin substitute visibly resembled an autograft. Eleven of the 19 ulcers (size 1–10 cm2) healed within 8 weeks after a single application of the skin substitute. The other eight larger (60–150 cm2) and/or complicated ulcers healed completely (n = 5) or continued to decrease substantially in size (n = 3) after the 8‐week follow‐up period. Wound healing occurred by direct take of the skin substitute (n = 12) and/or stimulation of granulation tissue/epithelialization (n = 7). Skin substitutes were very well tolerated and pain relief was immediate after application. Conclusions Application of this novel skin substitute provides a promising new therapy for healing chronic wounds resistant to conventional therapies.
Raynaud's Syndrome, Panic Disorder, and Mood Changes
Authors: Ronald Pies
Journal: JAMA (1985)

<h3>To the Editor.—</h3> Mueller and Allen<sup>1</sup>have described a group of seasonally depressed patients who are initially seen with Raynaud's phenomenon in a large percentage of cases. The authors speculate that dysmetabolism of melatonin and serotonin underlies these problems. I would like to report a case in which a Raynaudlike syndrome occurred in a patient with panic disorder and frequent mood swings. <h3>Report of a Case.—</h3> The patient was a 25-year-old white woman with a chief complaint of "panic attacks" over the course of many years, characterized by palpitations, shallow breathing, "hot and cold flashes," and the "feeling that I want to scream." These attacks seemed to increase in frequency during periods of elevated mood, and decreased during the patient's depressed phases. The patient gave a history of "poor circulation," noting that her hands would turn white, then "black or blue," then "black, with orange or white spots," in
Deadly radiation ignored in Iraq
Authors: P. Moszynski
Journal: BMJ (2003)

Villages surrounding Iraq's nuclear complex, Tuwaitha, just south of Baghdad, are contaminated with “deadly radiation” yet remain off limits to international inspectors, claims Greenpeace.

   ![][1] 

Iraqi children sleep outside the gates of the nuclear facility in Tuwaitha, 12 miles south of Baghdad

Credit: BULLIT MARQUEZ/AP

“The occupying forces have so far refused to allow the United Nations' nuclear experts, the International Atomic Energy Agency (IAEA), to carry out proper documentation and decontamination in Iraq,” said a Greenpeace spokesperson. “The US authorities in Baghdad have insisted on retaining responsibility …

 [1]: /embed/graphic-1.gif
DNA Copy Number Alterations in Endobronchial Squamous Metaplastic Lesions Predict Lung Cancer
Authors: Robert A. A. van Boerdonk, Thomas G. Sutedja, Peter J. F. Snijders, Emilie Reinen, Saskia M. Wilting, Mark A. van de Wiel, F. (Erik) B. J. M. Thunnissen, Sylvia Duin, Clarissa Kooi, Bauke Ylstra, Chris J. L. M. Meijer, Gerrit A. Meijer, Katrien Grünberg, Johannes M. A. Daniels, Pieter E. Postmus, Egbert F. Smit, Daniëlle A. M. Heideman
Journal: American Journal of Respiratory and Critical Care Medicine (2011)

Autofluorescence bronchoscopy (AFB) is a valid strategy for detecting premalignant endobronchial lesions. However, no biomarker can reliably predict lung cancer risk of subjects with AFB-visualized premalignant lesions.The present study set out to identify AFB-visualized squamous metaplastic (SqM) lesions with malignant potential by DNA copy number profiling.Regular AFB examinations in 474 subjects at risk of lung cancer identified six subjects with SqM lesions at baseline, and carcinoma in situ or carcinoma (carcinoma in situ or greater) at the initial SqM site at follow-up bronchoscopy. These progressive SqM lesions were compared for immunostaining pattern and array comparative genomic hybridization-based chromosomal profiles with 23 SqM lesions of subjects who remained cancer-free. Specific DNA copy number alterations (CNAs) linked to cancer risk were identified and accuracy of CNAs to predict endobronchial cancer in this series was determined.At baseline, p53, p63, and Ki-67 immunostaining were not predictive for a differential clinical outcome of SqM lesions. The mean number of CNAs in baseline SqM of cases was significantly higher compared with control subjects (P < 0.01). Chromosomal regions significantly more frequently altered in SqM of cases were 3p26.3-p11.1, 3q26.2-q29, 9p13.3-p13.2, and 17p13.3-p11.2 (family-wise error rate <0.10). CNAs were specifically detected at the site of future cancer. In cases, baseline-detected CNAs persisted in subsequent biopsies taken from the initial site, and levels increased toward cancer progression. In this series, a model based on CNAs at 3p26.3-p11.1, 3q26.2-29, and 6p25.3-24.3 predicted cancer with 97% accuracy.The data suggest that the presence of specific CNAs in SqM lesions predict endobronchial cancer.
Should Diabetes Be Considered a Coronary Heart Disease Risk Equivalent?
Authors: Lucinda Whiteley, Sandosh Padmanabhan, David Hole, Chris Isles
Journal: Diabetes Care (2005)

OBJECTIVE—The purpose of our study was to confirm or refute the view that diabetes be regarded as a coronary heart disease (CHD) risk equivalent and to test for sex differences in mortality. RESEARCH DESIGN AND METHODS—This was a prospective cohort study of 7,052 men and 8,354 women aged 45–64 years from Renfrew and Paisley, Scotland, who were first screened in 1972–1976 and followed for 25 years. All-cause mortality was calculated as death per 1,000 person-years. A Cox proportional hazards model was used to adjust survival for age, smoking habit, blood pressure, serum cholesterol, BMI, and social class. RESULTS—There were 192 deaths in 228 subjects with diabetes and 2,016 deaths in 3,076 subjects with CHD. The highest mortality was in the group with both diabetes and CHD (100.2 deaths/1,000 person-years in men, 93.6 in women) and the lowest in the group with neither (29.2 deaths/1,000 person-years in men, 19.4 in women). Men and women with diabetes only and CHD only formed an intermediate risk group. The adjusted hazard ratio (HR) for CHD mortality in men with diabetes only compared with men with CHD only was 1.17 (95% CI 0.78–1.74; P = 0.56). Corresponding HR for women was 1.97 (1.27–3.08; P = 0.003). CONCLUSIONS—Diabetes without previous CHD carries a lifetime risk of vascular death as high as that for CHD alone. Women may be at particular risk. Our data support the view that cardiovascular risk factors in diabetes should be treated as aggressively as in people with CHD.
Febrile Transfusion Reaction Following Initial Transfusion in a Man With Immunoblastic Lymphadenopathy and Granulocyte Autoantibodies
Authors: Harry M. Barnes, Man‐Chiu Poon, Shu Tsong Huang, Marcel E. Conrad, John Lin, Eoline I. McGowan
Journal: American Journal of Hematology (1983)

Abstract A 69‐year‐old man with immunoblastic lymphadenopathy and autoimmune hemolytic anemia who had no previous exposure to blood products developed a severe febrile nonhemolytic transfusion reaction following the initial infusion of packed red blood cells. The reaction recurred with transfusion of packed red blood cells, but not when freeze‐thawed red blood cells were used. Immunofluorescence techniques demonstrated granulocyte antibodies in his serum and on the surface of his granulocytes. Circulating immune complex, HLA, and platelet antibodies were not present. The granulocyte antibodies fluctuated in titers with disease activities, and could be completely removed from the serum by autologous granulocyte absorption. We conclude that our patient had granulocyte autoantibodies which probably produced febrile transfusion reactions.
From Form to Function—Better Imaging Techniques Extend Study of Living Systems
Authors: Bernadine Healy
Journal: JAMA (1992)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Screening for HIV Infection — Benefits and Costs
Authors: Thomas C. Quinn
Journal: New England Journal of Medicine (1992)

Now in its second decade, the human immunodeficiency virus (HIV) epidemic continues to escalate relentlessly. Approximately 1 million people in the United States are infected with HIV, and nearly a quarter of a million have been given diagnoses of the acquired immunodeficiency syndrome (AIDS).1 During the first eight years of the epidemic, 100,000 cases of AIDS were reported; another 100,000 were reported within the next two years.2 AIDS has caused 150,000 deaths in the United States and is now ranked as one of the leading causes of premature death for both men and women in this country,3 as well as . . .