tbuckley/cabot-search huggingface dataset:
Molecular Evolutionary Analyses of the Odorant-Binding Protein Gene Gp-9 in Fire Ants and Other Solenopsis Species
Authors: Michael J. B. Krieger, Kenneth G. Ross
Journal: Molecular Biology and Evolution (2005)

The fire ant Solenopsis invicta exists in two social forms, one with colonies headed by a single reproductive queen (monogyne form) and the other with colonies containing multiple queens (polygyne form). This variation in social organization is associated with variation at the gene Gp-9, with monogyne colonies harboring only the B allelic variant and polygyne colonies containing b-like variants as well. We generated new Gp-9 sequences from 15 Solenopsis species and combined these with previously published sequences to conduct a comprehensive, phylogenetically based study of the molecular evolution of this important gene. The exon/intron structure and the respective lengths of the five exons of Gp-9 are identical across all species examined, and we detected no evidence for intragenic recombination. These data conform to a previous suggestion that Gp-9 lies in a genomic region with low recombination, and they indicate that evolution of the coding region in Solenopsis has involved point substitutions only. Our results confirm a link between the presence of b-like alleles and the expression of polygyny in all South American fire ant species known to possess colonies of both social forms. Moreover, phylogenetic analyses show that b-like alleles comprise a derived clade of Gp-9 sequences within the socially polymorphic species, lending further support to the hypothesis that monogyny preceded polygyny in this group of fire ants. Site-specific maximum likelihood tests identified several amino acids that have experienced positive selection, two of which are adjacent to the inferred binding-pocket residues in the GP-9 protein. Four other binding-pocket residues are variable among fire ant species, although selection is not implicated in this variation. Branch-specific tests revealed strong positive selection on the stem lineage of the b-like allele clade, as expected if selection drove the amino acid replacements crucial to the expression of polygyne social organization. Such selection may have operated via the ligand-binding properties of GP-9, as one of the two amino acids uniquely shared by all b-like alleles is predicted to be a binding-pocket residue.
CTLA-4 is a second receptor for the B cell activation antigen B7.
Authors: P S Linsley, W Brady, M Urnes, L S Grosmaire, N K Damle, J A Ledbetter
Journal: The Journal of Experimental Medicine (1991)

Functional interactions between T and B lymphocytes are necessary for optimal activation of an immune response. Recently, the T lymphocyte receptor CD28 was shown to bind the B7 counter-receptor on activated B lymphocytes, and subsequently to costimulate interleukin 2 production and T cell proliferation. CTLA-4 is a predicted membrane receptor from cytotoxic T cells that is homologous to CD28 and whose gene maps to the same chromosomal band as the gene for CD28. It is not known, however, if CD28 and CTLA-4 also share functional properties. To investigate functional properties of CTLA-4, we have produced a soluble genetic fusion between the extracellular domain of CTLA-4 and an immunoglobulin C gamma chain. Here, we show that the fusion protein encoded by this construct, CTLA4Ig, bound specifically to B7-transfected Chinese hamster ovary cells and to lymphoblastoid cells. CTLA4Ig also immunoprecipitated B7 from cell surface 125I-labeled extracts of these cells. The avidity of 125I-labeled B7Ig fusion protein for immobilized CTLA4Ig was estimated (Kd approximately 12 nM). Finally, we show that CTLA4Ig was a potent inhibitor of in vitro immune responses dependent upon cellular interactions between T and B lymphocytes. These findings provide direct evidence that, like its structural homologue CD28, CTLA-4 is able to bind the B7 counter-receptor on activated B cells. Lymphocyte interactions involving the B7 counter-receptor are functionally important for alloantigen responses in vitro.
126 Complications in the donor after right-lobe adult living donor liver transplantation (LDLT)
Authors: J. Ferrer, J. Fuster, R. Charco, E. Bombuy, C. Fondevila, G. Alvarez, G. Rodriguez-Laiz, A. Amador, M. Sala, J.M. Llovet, M. Navasa, A. Rimola, J. Bruix, J.C. Garcia-Valdecasas
Journal: Journal of Hepatology (2004)
Restricted Expression of an Adenoviral Vector Encoding Fas Ligand (CD95L) Enhances Safety for Cancer Gene Therapy
Authors: Kazunori Aoki, Levent M. Akyürek, Hong San, Kwanyee Leung, Michael S. Parmacek, Elizabeth G. Nabel, Gary J. Nabel
Journal: Molecular Therapy (2000)

Gene transfer of Fas ligand (CD95L) using adenoviral vectors has been shown to generate apoptotic responses and potent inflammatory reactions that can be used to induce the regression of malignancies in vivo, but these vectors also cause significant hepatotoxicity that may limit their clinical utility. Here we describe an adenoviral vector encoding CD95L with restricted gene expression that reduces its toxicity in vivo. Preclinical efficacy and gene expression studies of lineage-restricted CD95L adenoviral vectors were performed. To enhance its cytotoxicity and reduce potential systemic effects, a noncleavable CD95L was made by deleting a segment containing the cleavage site (CD95LδQP). Higher CD95L expression of this mutant was observed on the tumor cell surface, together with a reduction in the release of soluble CD95L. This CD95L cleavage mutant was then expressed under control of a smooth muscle-specific promoter, SM22α, and analyzed for its ability to suppress the growth of tumors of smooth muscle origin in vivo. Growth of human leiomyosarcomas but not gliomas was inhibited after ADV gene transfer into tumor-bearing immunodeficient mice. In contrast to viral promoters, in which mortality was uniformly seen after injection of 1012 particles, no significant hepatic injury or systemic toxicity was observed in mice, and the maximum tolerated dose was increased ≥10- to 100-fold. These findings suggest that restricted specificity of adenoviral CD95L gene expression enhances the safety of this approach for cancer gene therapy.
Sudden, unexpected death in avid dieters using the liquid-protein-modified-fast diet. Observations in 17 patients and the role of the prolonged QT interval.
Authors: J M Isner, H E Sours, A L Paris, V J Ferrans, W C Roberts
Journal: Circulation (1979)

Clinical and morphologic findings are described in 17 patients who died suddenly and unex- pectedly during or shortly after use of the liquid-protein-modified-fast diet.Of the 17 patients, 16 were women, most were young (average age 37 years), and most lost a massive amount of weight (average 41 kg or 35% of their prediet weight) over a short period of time (average 5 months).Eight had one or more episodes of syncope.Multiple-lead ECGs were recorded in 10 patients.All had normal sinus rhythm; all had episodes of ventricular tachycardia; nine and possibly 10 patients had prolongation of the QT interval, unassociated with the recognized causes of QT interval prolongation in at least seven of the nine patients; and nine had diminished amplitude of the QRS complexes ("low voltage").Histologic study of left ventricular myocardium in 14 pa- tients disclosed attenuated myocardial fibers in 12, increased lipofuscin pigment in 11, and mononuclear-cell myocarditis in one.Similar histologic findings, however, also were found in 16 cachectic control subjects studied in similar fashion, but ECGs in them showed no prolongation of QT intervals or episodes of ventricular tachycardia.Thus, semistarvation, particularly in the face of antecedent obesity, is a cause of acquired QT interval prolongation, and repeated ECGs are recommended in patients on semistarvation diets for treatment of obesity.IN 1976 a book entitled The Last Chance Diet' was published and almost immediately, several liquid-protein-modified-fast (LPMF) diets became very popular and fashionable as means of rapid weight reduction.These diets were intended to serve as the dieter's only source of calories.Between January 1, 1977 and December 31, 1977, it was estimated that more than 100,000 persons had used one or more of the LPMF diets as their sole source of nourishment for at least 1 month.2By August 1977, however, sudden death in several young LPMF diet users was reported to either the Food and Drug Administration (FDA) or the Center for Disease Control (CDC), and between July 1977 and January 1978 at least 60 deaths among avid users of the LPMF diet were reported to the FDA and CDC.'In 28 of these 60 patients, there was clinical or
Cell Microenvironment: Confinement and Deformation of Single Cells and Their Nuclei Inside Size‐Adapted Microtubes (Adv. Healthcare Mater. 11/2014)
Authors: Britta Koch, Samuel Sanchez, Christine K. Schmidt, Anka Swiersy, Stephen P. Jackson, Oliver G. Schmidt
Journal: Advanced Healthcare Materials (2014)

Biofunctionalized glass microtube structures serve as transparent cell culture scaffolds and are fabricated to impose spatial confinement on single cells on page 1753. S. Sanchez, C. K. Schmidt, and colleagues demonstrate the influence of the resulting cell deformation on the integrity of the nucleus of human osteosarcoma cells and on cell division. Their findings underline the versatility of the rolled-up microtubes for mimicking space restrictions present in physiological tissues.
Regulating the TRAIL of Destruction: How A20 Protects Glioblastomas from TRAIL-Mediated Death
Authors: Inge Verbrugge, Ricky W. Johnstone
Journal: Cancer Discovery (2012)

Abstract In this issue of Cancer Discovery, Bellail and colleagues unravel how overexpression of the ubiquitin-modifying enzyme A20 results in TNF-related apoptosis-inducing ligand (TRAIL) resistance in glioblastoma. After TRAIL receptor stimulation, A20 mediates the polyubiquitination of RIP1 at the TRAIL receptor tail, resulting in the interaction of the polyubiquin chain to procaspase-8 that is recruited to the TRAIL-bound receptors. The inability of ubiquitin-bound procaspase-8 to be dimerized and activated prevents the execution of the apoptotic program. Cancer Discovery; 2(2); 112–4. ©2012 AACR. Commentary on Bellail et al., p. 140.
Peer Review in Prague
Authors: Drummond Rennie
Journal: JAMA (1995)

When science operates well, the publication of research occurs after expert criticism and revision and is itself followed by unrestrained criticism from the scientists' peers: the readers. The existence of a rigorous peer review system is the best sign that science is functioning in an appropriate and nurturing environment. Recognizing the central position of editorial peer review in the scientific process, we at<i>JAMA</i>announced in 1986 that we intended to host a conference in 3 years at which the results of research into editorial peer review would be presented and discussed.<sup>1</sup>We received an enthusiastic response, and in 1989 the First Congress on Peer Review in Biomedical Publication was held in Chicago, under the auspices of the American Medical Association. Manuscripts developed from the research presentations were published in 1990.<sup>2</sup>In 1993 we held another conference in Chicago, receiving twice as many abstracts from which to choose
How Safe Is Long-term Prenatal Glucocorticoid Treatment?
Authors: Jonathan R. Seckl
Journal: JAMA (1997)

GLUCOCORTICOIDS are commonly used in obstetric practice, both for maternal immunosuppression and for shortterm treatment of the fetus, usually to accelerate lung maturation when there is threatened preterm delivery. The immediate benefits of short-term prepartum glucocorticoid therapy in maturing the fetal lung and reducing neonatal mortality are clearly supported by controlled studies.<sup>1,2</sup>Recently, long-term prenatal therapy of fetuses potentially affected with congenital adrenal hyperplasia (CAH) has been advocated,<sup>3,4</sup>but the safety of this experimental therapy has not been established in rigorously controlled trials. See also p 1073. Congenital adrenal hyperplasia is a recessively inherited disease in which a disordered steroidogenic enzyme, usually P450c21 (21-hydroxylase), diverts adrenal steroid synthesis away from cortisol toward androgens.<sup>5</sup>In utero, low fetal plasma cortisol levels stimulate increased corticotropin (ACTH) secretion, which in turn stimulates further adrenal steroidogenesis, resulting in even more androgen synthesis. In an XY male fetus, these adrenal androgens have
Oral contraceptives and death from myocardial infarction.
Authors: J I Mann, W H Inman
Journal: BMJ (1975)

We investigated 219 deaths from myocardial infarction in women under the age of 50. Their histories were compared with those of living age-matched controls selected from the same general practices. The frequency of use of oral contraceptives during the month before death was significantly greater in the group with infarction than during the corresponding month in the control group and the average duration of use was longer. No information of cigarette smoking was available but the proportion of women being treated for hypertension or diabetes was greater among those who died than among the controls. This did not alter the overall conclusion that the risk of fatal myocardial infarction was greater in the women using oral contraceptives, particularly in the older age groups.
Production of a Low Molecular Weight Eosinophil Polymorphonuclear Leukocyte Chemotactic Factor by Anaplastic Squamous Cell Carcinomas of Human Lung
Authors: Edward J. Goetzl, Armen H. Tashjian, Robert H. Rubin, K. Frank Austen
Journal: Journal of Clinical Investigation (1978)

A peptide of approximately 300-400 daltons exhibiting in vitro chemotactic activity for human polymorphonuclear (PMN) leukocytes, with a preference for the eosinophil series, was isolated from extracts of anaplastic lung carcinomas of the large squamous cell type obtained from three patients with marked peripheral blood hypereosinophilia and eosinophilic infiltration of the tumors and surrounding normal pulmonary tissues. This chemotactic factor was termed ECF-LSC (eosinophil chemotactic factor of lung squamous cell carcinoma). ECF-LSC appeared in the urine of two of the patients in increasing quantities late in the course of their disease and was also elaborated by long-term cultures of dispersed tumor cells from the same two patients. Three anaplastic large cell bronchogenic carcinomas which were not associated with tumor tissue or peripheral blood eosinophilia, a bronchogenic adenocarcinoma from a patient with only peripheral eosinophilia, and a renal cell carcinoma metastatic to the lungs and associated with transient pleural tissue and fluid eosinophilia were all devoid of ECF-LSC. ECF-LSC from tumor tissue extracts, urine, and tumor cell culture medium was comparable to the mast cell-associated tetrapeptides of the eosinophil chemotactic factor of anaphylaxis (ECF-A) in size, but eluted from Dowex-1 at pH 5.0-3.5 in contrast to the more acidic ECF-A tetrapeptides which eluted at pH 3.2-2.2 ECF-LSC, like the tetrapeptides of ECF-A, had a secondary chemotactic activity for neutrophil PMN leukocytes, but not mononuclear leukocytes, and deactivated both eosinophil and neutrophil PMN leukocytes so that they would not respond to a subsequent in vitro chemotactic stimulus. Eosinophils from the two patients with urinary excretion of ECF-LSC and the highest concentrations in tumor extracts were hyporesponsive in vitro to homologous and heterologous chemotactic stimuli, suggesting that ECF-LSC had deactivated the eosinophils in vivo.
Lin28B/Let-7 Regulates Expression of Oct4 and Sox2 and Reprograms Oral Squamous Cell Carcinoma Cells to a Stem-like State
Authors: Chian-Shiu Chien, Mong-Lien Wang, Pen-Yuan Chu, Yuh-Lih Chang, Wei-Hsiu Liu, Cheng-Chia Yu, Yuan-Tzu Lan, Pin-I. Huang, Yi-Yen Lee, Yi-Wei Chen, Wen-Liang Lo, Shih-Hwa Chiou
Journal: Cancer Research (2015)

Lin28, a key factor for cellular reprogramming and generation of induced pluripotent stem cell (iPSC), makes a critical contribution to tumorigenicity by suppressing Let-7. However, it is unclear whether Lin28 is involved in regulating cancer stem-like cells (CSC), including in oral squamous carcinoma cells (OSCC). In this study, we demonstrate a correlation between high levels of Lin28B, Oct4, and Sox2, and a high percentage of CD44(+)ALDH1(+) CSC in OSCC. Ectopic Lin28B expression in CD44(-)ALDH1(-)/OSCC cells was sufficient to enhance Oct4/Sox2 expression and CSC properties, whereas Let7 co-overexpression effectively reversed these phenomena. We identified ARID3B and HMGA2 as downstream effectors of Lin28B/Let7 signaling in regulating endogenous Oct4 and Sox2 expression. Let7 targeted the 3' untranslated region of ARID3B and HMGA2 and suppressed their expression, whereas ARID3B and HMGA2 increased the transcription of Oct4 and Sox2, respectively, through promoter binding. Chromatin immunoprecipitation assays revealed a direct association between ARID3B and a specific ARID3B-binding sequence in the Oct4 promoter. Notably, by modulating Oct4/Sox2 expression, the Lin28B-Let7 pathway not only regulated stemness properties in OSCC but also determined the efficiency by which normal human oral keratinocytes could be reprogrammed to iPSC. Clinically, a Lin28B(high)-Let7(low) expression pattern was highly correlated with high levels of ARID3B, HMGA2, OCT4, and SOX2 expression in OSCC specimens. Taken together, our results show how Lin28B/Let7 regulates key cancer stem-like properties in oral squamous cancers.
Three More Cancer Centers Receive Comprehensive Status
Authors: M. E. Newman
Journal: JNCI Journal of the National Cancer Institute (1990)

Journal Article Three More Cancer Centers Receive Comprehensive Status Get access Michael E. Newman Michael E. Newman Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 82, Issue 17, 5 September 1990, Page 1389, https://doi.org/10.1093/jnci/82.17.1389 Published: 05 September 1990
Mitochondrial ATP-sensitive K+channels play a role in cardioprotection by Na+-H+exchange inhibition against ischemia/reperfusion injury
Authors: Tetsuji Miura, Yongge Liu, Mahiko Goto, Akihito Tsuchida, Takayuki Miki, Atsushi Nakano, Yasuhiro Nishino, Yoshito Ohnuma, Kazuaki Shimamoto
Journal: Journal of the American College of Cardiology (2001)
Adipose tissue metabolism in obesity: Lipase action in vivo before and after a mixed meal
Authors: S.W. Coppack, R.D. Evans, R.M. Fisher, K.N. Frayn, G.F. Gibbons, S.M. Humphreys, M.L. Kirk, J.L. Potts, T.D.R. Hockaday
Journal: Metabolism (1992)
The Method in Psychotherapy
Authors: J. Fusswerk-Fursay
Journal: Psychotherapy and Psychosomatics (1974)

Research Articles| February 12 2010 The Method in Psychotherapy Subject Area: Psychiatry and Psychology J. Fusswerk-Fursay J. Fusswerk-Fursay Paris Search for other works by this author on: This Site PubMed Google Scholar Psychother Psychosom (1974) 24 (4-6): 439–443. https://doi.org/10.1159/000286781 Article history Published Online: February 12 2010 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation J. Fusswerk-Fursay; The Method in Psychotherapy. Psychother Psychosom 31 December 1974; 24 (4-6): 439–443. https://doi.org/10.1159/000286781 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsPsychotherapy and Psychosomatics Search Advanced Search This content is only available via PDF. 1974Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.
Fenofibrate induces plaque regression in hypercholesterolemic atherosclerotic rabbits: In vivo demonstration by high-resolution magnetic resource imaging
Authors: Roberto Corti, Julio I. Osende, John T. Fallon, Urooj Zafar, Valentin Fuster, Gabor Mizsei, Hani Jneid, Juan J. Badimon
Journal: Journal of the American College of Cardiology (2003)
The Coal-Powered Heart
Authors: C. E. Butterworth
Journal: JAMA (1974)

In the midst of all the current discussions about the energy crisis, the concept of food as a form and source of energy generally has been overlooked. There was a hint in 1972 of possible trouble ahead when a shortage of fuel threatened to prevent the drying of tons of grain for storage in the north central states. Then last summer, even before the Yom Kippur war, headlines warned that a gasoline shortage might interfere with the harvesting of crops. Fortunately, these events did not develop into a major crisis, and they passed unnoticed because there was no abrupt change in the supply or price of food. Attention was diverted because the public was not seriously affected in either the stomach or the pocketbook. Nevertheless, there can be little doubt that these developments have contributed to the current upward trend in food prices for the American family. The recent cutback
FURTHER EXPERIENCE OF THE SPECIFIC CURATIVE ACTION IN AMOEBIC DISEASE OF HYPODERMIC INJECTIONS OF SOLUBLE SALTS OF EMETINE
Authors: L. Rogers
Journal: BMJ (1912)

In the British Medical Journal of June 22nd * last I recorded cases illustrative of acute and chronic amoebic dysentery and acute hepatitis respectively in patients who were unable to take ipecacuanha by the mouth, but were rapidly cured by subcutaneous injections of soluble salts of emetine, without the production of vomiting or marked nausea, which are such serious drawbacks to the use of full doses of ipecacuanha by the mouth. During the two months which have elapsed since that note was written I have had frequent opportunities of using the new treatment in suitable cases, thanks mainly to the kindness of my I.M.S. colleagues in the'different Calcutta hospitals, to whom I tender my best thanks. The results have boon more than confirmatory of my first very favourable im pressions, and leave no possible doubt as to the great practical importance of the discovery. Some of them are, indeed, so surprising that they must be seen to be fully appreciated, as words can convey but a feeble impression of the rapidity and completeness of the recoveries effected in very grave cases. In dealing with the more extensive material now available it will be well to classify the cases into different clinical groups.